LOCUS       DATMZQ010000001         8889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4833692, whole genome shotgun sequence.
ACCESSION   DATMZQ010000001 DATMZQ010000000
VERSION     DATMZQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4833692"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VN496_00005"
     CDS             complement(<1..1260)
                     /locus_tag="VN496_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF15350.1"
                     /translation="MPFDLLIRNGLVIDGTGAPGRLADVAVSGGKIVEIGKGIGSARQ
                     TIDASDCVVSPGFIDPHTHYDAQICWDGAVTPSSWHGVTSVVMGNCGVGIAPCLPASR
                     EIAMHDLVNVEAIPYDVLKQGISWDWETFPEYMDAAARRQPVLNLAFLAPLTPFRHYV
                     MGEASMERAANPEETAGIAALLGEAMDAGAFGFSSTILNQHLGFGGRPLACRNASDAE
                     LKAYANVLKQKNKGAIEVALTRQIGVLEPDQCETLDLLLDESGRPVTFIALFDRDDIP
                     EAVRDTLRRAAPMIAKGARPQTSPLPLTREIDMRSPFSFAAFPSWKRVFEDKSAPAQK
                     AVYRDVQFRNQFREELKNPLAFGNWDRITLHEAHSPELKRFEGMTVAQVARAQGKDGV
                     DAFLDLTLDDDLALEFTMTSFNTRVDRM"
     gene            complement(1273..2577)
                     /locus_tag="VN496_00010"
     CDS             complement(1273..2577)
                     /locus_tag="VN496_00010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF15351.1"
                     /translation="MNCTRVAAVLLAVTVVLRAAALHAQALPAAAPEAVGMSSQRLHN
                     ISGAFRKEIDKGNLPGVVVMVARKGKLVYSDAIGSQNKATGKPMTKDAMFRIYSMTKP
                     LISVAAMMLVEDGKMELTDPVSKFLPALKNMQVSVAKVDAEFARTTYALAPAEREMTV
                     QDLMRHTAGLAYANLTQNKAVVDAYTKGGVAADLRGLTPEEEVERVAQAPLIHQPGSV
                     WEYSIATDVLGRVVEAASGKRLADFLAERLFMPLGMKDTAFFVAADKMDRLAEALAVD
                     ASTGKPNELYDVSSIPKNDSGGAGGVSTAADYLRFAQMMANGGQLDGKRYLSRTSVEL
                     MTSDHLGTRIQALSPMTPGEVLLNTPGYTFGLGFAVRPAPGIAGVPGSMGEFMWGGYA
                     GTYFWVDPKEQLVAVYMTQAPSPIRSYFRKLFKQLVYAAIVD"
     gene            complement(2698..3240)
                     /locus_tag="VN496_00015"
     CDS             complement(2698..3240)
                     /locus_tag="VN496_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HXF15352.1"
                     /translation="MWTIVKQIARAGICTQAVPEFSDALRLEAQRIQRDILDILGRAL
                     TIRQVDAGSCNGCELEIHALNNPYYNIEGLGIRFVASPRHADMLLVTGPVSRNMEVAL
                     KRTFEATPDPKLVVAVGECGCNGGIFGESYASCGRVSNVIPVDLAVPGCPPAPVEILR
                     GILTAVRRCRPKSEDKPGGS"
     gene            complement(3240..4799)
                     /locus_tag="VN496_00020"
     CDS             complement(3240..4799)
                     /locus_tag="VN496_00020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXF15353.1"
                     /translation="MRLSGLTIDFEQLAAPLPVLYGAVNAEDWLAVPMTLVKSGGRLV
                     SIWGTECGDGEPGQYAICAAYAAREGLLWIELPLAGRTTYPDLTPLFPCANRMQRAVA
                     DLLGIAAQDASDQRSWLNHGAWSPAHFPLRQKSSGDAIASDRQPGDYPFVRVEGDGVH
                     EIPVGPVHAGIIEPGHFRFSIVGEKVLRLEQRLGYTHKGIDKRFEQLAPMEAHRLAGR
                     VSGDSTVAFAWAYCMALESAAGCKISARGAWLRALMLERERVANHLGDLGALGNDASF
                     SFGLAQFSRLREDWLRTSAAIFGHRLMMDCVVPGGVATDIDRAAIDLIDQQCDAVERE
                     VQILRNIYDEHAGLQDRFITTGRVQPALAAQLGLTGLAGRASGQAADLRSDHPFPPYD
                     ALEVKMATHRNGDVAARVTVRFEETIESLRLIRALCKGLPGGAVMTELELPATGSRGA
                     GWVEGWRGEVLVILELEADANGSHIRRCHCHDPSWQNWPVLEHAVIGNIVPDFPLINK
                     SFNLSYSGHDL"
     gene            complement(4804..6258)
                     /locus_tag="VN496_00025"
     CDS             complement(4804..6258)
                     /locus_tag="VN496_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HXF15354.1"
                     /translation="MDILFFVFGIPLLGSVILAFTGHRNFAPEINVAFSFGTFIAACA
                     LTVQVISMGPVFAWNRAFFIDPLNVFIVTLTAFVGLTTALFSRPYMRVERDHGKMTPR
                     RLRLYHSMYQSFSFTMLLALTTNNMGILWVAMEAATLTTVLLVSVYRTAARLEAAWKY
                     FILCGVGIAQALFGTVLLYMAAEKVVGPEGGALIWTNLDAVKAQLDPTIITLAFAFLF
                     IGYGTKVGLVPLHNWLPDAHAEGPTPVSAILSGLLLNVALYAILRCKVLTDGALANAL
                     TGNLMMGFGLLSVVVAAFFLSRQKDVKRMFAYSSIEHMGLITFAFGMGGPIATFAGLL
                     HMTVHSLVKSAIFFVVGHAAQKAGTQIMEEIRGLVKVNPMVGWGLMLGSLAILGMPPF
                     GVFASEFLILTTAMREHPWATPLLLLALGVAFASVLSRVLPMVFGETSVKPLFHPPAV
                     IPVFIHLGLALLLGLYIPTYLDTWYRQAARMIGG"
     gene            complement(6259..6918)
                     /locus_tag="VN496_00030"
     CDS             complement(6259..6918)
                     /locus_tag="VN496_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HXF15355.1"
                     /translation="MSGIASQLINLFAAVLLLLAFAMISQRRVLSLIYLFTLQGATLA
                     AATAVVGYATHQPHLYISASITLVLKVFLIPWLLHRVIDRLNVRWDVETLINIPTTML
                     IGILLVILAFYLALPVSQLSSSIARGTLGIALACMLLSFMMMITRSKAVPQVIGFLSM
                     ENGLFFAATAATYGMPMVVELGIALDVLVGILILGVFMFQIREQFDSLDIRHLEKLKE
                     D"
     gene            complement(6923..7870)
                     /locus_tag="VN496_00035"
     CDS             complement(6923..7870)
                     /locus_tag="VN496_00035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXF15356.1"
                     /translation="MNPLGVISQLLAIIVALALAPLLTGWVNQWRAWLQNKSAPSLLQ
                     QYRMLHKLFNKDSVVAVHASPFFRIAPYIIFGCMTLACAIIPMLSTDLPLASAADAIA
                     LVGLFALARVFISLAAMDVGTAFGNLGARREMLIGFLAEPALLMVLFTAALISNSTSL
                     TSIVDTLGGRDLAIFPSLAFAGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEY
                     SGRHLALVEWAASLKLFAYSCIGLALFIPWGMADAQAPVALLLALPVLILKLAVGGVL
                     LAVLETVSAKMRIFRVPEFLCTAFLLAVIAMLVHILLGA"
     gene            complement(7867..>8889)
                     /locus_tag="VN496_00040"
     CDS             complement(7867..>8889)
                     /locus_tag="VN496_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXF15357.1"
                     /translation="FGMTPMATLALTAALYHVVSHAFFKSLLFVSTGAVLHATSQRSL
                     GKLGGLIRYMPWVAWICLIGVLTSAGLPPLSGFVSEWLLLQGFLFTPELPSSFLNMLI
                     PISAALVALIAALAGYTMVKFFGIIFLGQPREEQLAHVHDAGRWERAGMIWLACGCVL
                     LGLLPAQFIQFIDPVTRQLTGAGLGSTVAANGWLLIPVAAERASYGPIIFLAGVAASF
                     ALAFLLVRKLYHGRLRRSAPWDCGFPWLTPRMQDTAEGFGQPIRQIFESLFRVERQLP
                     TPFDAQPRYRVSIGDHFWYWLYLPVAHVTERMARLIGLMQQGRISVYLLYSFVTLIAL
                     LVWVTR"
BASE COUNT         1963 a         2763 c         2609 g         1554 t
ORIGIN      
        1 catgcgatcg acgcgcgtat tgaaggaagt catggtgaat tcgagcgcca ggtcatcgtc
       61 cagggtcaga tcgaggaacg catcgacacc atctttgccc tgcgcgcggg caacttgcgc
      121 caccgtcatg ccttcaaaac gcttcagttc cggagaatgc gcctcgtgca gcgtgatgcg
      181 gtcccaattg ccaaaggcaa gcggattctt cagctcttcc cggaattggt tgcggaactg
      241 cacatcacga taaaccgctt tctgcgctgg cgcggatttg tcctcgaaca cccgcttcca
      301 ggacggaaac gccgcaaacg agaacggact gcgcatatcg atctcgcgcg tcagcggtaa
      361 cggagaagtc tgcgggcggg cacctttggc gatcatcggt gccgcccggc gcagcgtatc
      421 gcgcactgct tccggaatgt cgtcccgatc gaacagcgcg atgaacgtca ccggacggcc
      481 actttcatcg agcagcagat cgagcgtctc gcactgatca ggctcgagta cgccgatctg
      541 ccgtgtcaga gcgacttcga ttgcgccctt atttttttgc ttgagcacgt tcgcgtacgc
      601 tttcaattcg gcatcactgg cattacggca cgccagcggg cgcccgccga agccgagatg
      661 ctggttcagg atcgtactcg aaaaaccaaa tgcgccggca tccatcgctt cgcccagcag
      721 cgccgcaatg cccgcagtct cctcaggatt ggcggcacgt tccatggatg cttctcccat
      781 gacgtaatgc cggaacggcg tcagcggcgc cagaaaggcc agattcagca cgggctggcg
      841 gcgcgctgcg gcgtccatgt attcaggaaa agtttcccag tcccaactga tgccctgctt
      901 gagcacgtcg tagggaatgg cctcaacatt cacaagatcg tgcatcgcga tttctcggga
      961 agcgggcagg catggcgcaa tgccgacgcc gcaattgccc atgaccacgc tggtgacacc
     1021 gtgccaagat gaaggggtga ccgcgccgtc ccagcaaatc tgcgcgtcgt aatgcgtgtg
     1081 cgggtcgatg aatccggggg agaccacaca gtccgatgca tcgatggttt gccgcgcgct
     1141 gccaatgcct ttgccgatct caacgatttt tccgccgctc acggcgacat ccgcaagccg
     1201 gcccggggcg cctgtgccgt cgatgacgag gccattgcga atcagcaaat cgaaaggcat
     1261 gtgccggatg acctaatcga caatcgccgc gtagaccagc tgcttgaaca gcttgcggaa
     1321 ataggagcgg atcgggctgg gcgcctgcgt catgtagacg gccaccagct gttcttttgg
     1381 atcgacccag aaatacgtcc cggcgtagcc gccccacatg aactccccca tcgaacccgg
     1441 caccccggcg atgcccggtg ccggccgcac ggcgaagccg agaccaaagg tgtagcccgg
     1501 cgtattcaac agtacttcgc ccggtgtcat tggcgacaac gcctgaatgc gcgtgccgag
     1561 atgatcggag gtcataagct ctacgctcgt gcggctcagg tagcgcttgc cgtcaagctg
     1621 accgccattg gccatcatct gcgcaaagcg cagataatcg gccgccgtcg acacgccgcc
     1681 cgcgccgccc gaatcgttct tcggtatcga gctgacgtca tagagctcgt tcggcttgcc
     1741 ggtggaggca tccaccgcca atgcttcggc cagacgatcc atcttgtcgg ccgccacgaa
     1801 gaatgcggtg tccttcatgc cgagcggcat gaacaggcgc tcagccagaa aatcagccag
     1861 ccgctttccc gatgcggcct cgaccacgcg tcctaatacg tccgtcgcaa tgctgtattc
     1921 ccaaacgctg cccggctgat gaatcagcgg cgcttgcgca acccgctcga cttcctcttc
     1981 cggggtgagt ccacgcagat cggcggccac gccacccttg gtgtaagcgt ccacgacggc
     2041 cttgttctgc gtgaggttcg cgtaagcgag gccagccgta tgccgcatca gatcctgcac
     2101 agtcatttcg cgctcggccg gcgccagtgc ataagtcgtc ctggcgaatt ctgcatcgac
     2161 tttggcgacg ctaacctgca tgtttttgag ggccggcagg aatttcgaca ccggatcggt
     2221 caattccatc ttgccgtctt ccaccagcat catggctgcc accgagatca gtggcttggt
     2281 catcgagtag atgcgaaaca ttgcatcctt ggtcatcggt ttgccagtcg ccttgttctg
     2341 ggacccgatg gcatccgagt acaccagctt gcctttgcgg gcgaccatca cgaccacgcc
     2401 gggcaaattg cctttgtcaa tttccttacg gaaggcaccc gaaatgttat gcagccgttg
     2461 cgacgacatg ccgaccgctt ccggtgcggc ggccggcaat gcctgcgcat gcagcgccgc
     2521 ggcccgaaga acaaccgtga cggcaagcaa tactgcagcg acacgcgtgc aattcatctg
     2581 tttatcctcc cgcccgttcg gggctattct tgttggtgcg agggaggtta ggctgcggac
     2641 ctgcgccatg tcaatgcaaa gcgccgtgta cttcaccagg tagcgcgtgc gccgatatca
     2701 ggagccgccc ggtttgtctt ccgattttgg gcgacagcgc cttaccgccg tcaggatgcc
     2761 gcgaagaatc tcgacaggag ccggcgggca gcccggcacc gcgagatcga cgggtatgac
     2821 gttcgacacg cggccgcagc tcgcgtagct ctcgccgaag atgccgccgt tgcagccgca
     2881 ctccccaact gcgaccacca gcttgggatc tggcgtggcc tcgaacgtgc gtttcagggc
     2941 cacttccatg ttgcgcgaaa ccggtccggt gaccagcaac atgtcggcgt gacgcggact
     3001 cgccacaaac cggatgccca gcccttcgat gttgtagtag ggattgttca aagcatggat
     3061 ttccagttcg cagccattgc acgagccagc atccacctgc cggatggtca gcgcgcgacc
     3121 cagaatgtca aggatgtcac gctggatgcg ctgcgcctca agtcgcagcg catcgctgaa
     3181 ttcgggaacc gcttgcgtgc atattccagc acgcgcgatc tgtttgacga tcgtccacat
     3241 cagaggtcgt gtcccgaata gctcaagttg aacgacttgt tgataagcgg gaaatcggga
     3301 acgatgtttc cgatcaccgc gtgttcaagc accggccaat tctgccagga cggatcgtgg
     3361 cagtgacagc gccggatgtg gctgccattt gcgtccgctt caagctcgag gataaccagt
     3421 acttcgccgc gccatccctc tacccatccc gcgccgcgcg atcctgtcgc gggcagctcc
     3481 agttccgtca ttactgcgcc gccgggaaga cctttgcaca gcgcgcgaat aagtcgcaat
     3541 gattcgatcg tctcttcaaa acgcaccgtt accctggccg cgacgtcgcc gttgcggtgg
     3601 gtcgccattt tgacttccag tgcgtcatag ggcggaaacg gatgatcgct gcgcaaatct
     3661 gcggcctggc cgctcgctcg tccggccagc ccggtcagcc ccaactgcgc agccagtgcc
     3721 ggctgcacgc ggccggtcgt gatgaagcgg tcctgcaaac cggcgtgctc gtcgtagatg
     3781 ttgcgcagga tctgcacttc acgctcgacg gcgtcgcatt gctggtcgat aagatcgatc
     3841 gctgcgcggt ctatatcagt cgcgacgcca ccgggcacga cgcaatccat catcaagcgg
     3901 tggccgaaga tggcagccga cgtgcgcagc cagtcttccc gcaagcgcga aaattgcgca
     3961 agaccaaagc taaacgaggc atcgttaccg agcgcgccca ggtcgccgag atggttggcc
     4021 acacgctcgc gctccagcat gagtgctctc agccatgcac cgcgagcaga aatcttgcaa
     4081 ccagccgccg attccagcgc catgcaataa gcccacgcaa aggccacggt ggaatcgccg
     4141 gaaactcgcc ctgccagtcg atgcgcttcc attggcgcca attgttcgaa acgcttgtcg
     4201 atgcccttgt gcgtgtagcc gaggcgttgc tccagccgca ggactttctc gccgacgatg
     4261 gaaaagcgga agtgtccagg ctcgatgatg cccgcatgaa ccggccccac cggaatttcg
     4321 tgcacgccat cgccttctac gcgcacgaac ggatagtcgc cgggttggcg atcggaagca
     4381 atcgcgtcgc cggaagattt ttgtcgcagc ggaaagtggg caggcgacca ggcgccgtga
     4441 ttgagccacg agcgctggtc gctggcgtct tgcgcagcga tgccgagcaa atccgcgacc
     4501 gcgcgctgca ttcggttggc acacggaaac agcggcgtca gatcgggata agtcgtgcgc
     4561 cccgcaagcg gcagttcaat ccataacagt ccctcgcgtg cggcataggc ggcgcagatg
     4621 gcatattgac ctggctcccc gtcgccgcat tcggttcccc atatggaaac cagtcgccct
     4681 ccacttttga ccagcgtcat tggcactgcc aaccagtcct ccgcgttgac agcgccgtac
     4741 agcaccggca aaggcgctgc gagttgctcg aaatcgatgg tcaatccgga aaggcgcatt
     4801 gcatcagcct ccgatcatcc gcgccgcctg ccgataccag gtgtcgaggt aggtcgggat
     4861 atacaaaccg agcagcagcg ccagcccgag atggatgaac accggaatca ccgctggcgg
     4921 atgaaacagc ggcttgaccg aagtctcccc aaataccatc ggcagcacgc ggctcaacac
     4981 cgaagcgaaa gccaccccaa gcgcgagcag cagcaatgga gtcgcccagg gatgctcgcg
     5041 catggccgtg gtcaggatca gaaactcgct ggcgaaaacg ccgaaaggtg gcatgccgag
     5101 aatcgcgagc gatccgagca tcagtcccca accaaccatg ggattgactt tcacgaggcc
     5161 gcgaatttct tccatgattt gcgtgcccgc tttctgagcc gcgtgaccca caacgaagaa
     5221 gatcgcggat ttgaccagcg aatgcacggt catgtgcagc aggccggcaa aggtcgcaat
     5281 cggtccgccc atgccaaaag caaacgtaat gagccccatg tgttcgatgg aagagtacgc
     5341 aaacatgcgc ttgacgtctt tctgtcgcga caggaaaaac gccgccacta ccaccgacag
     5401 caaaccgaag cccatcatca ggttgccggt cagcgcgttg gccaacgcgc cgtcggtaag
     5461 caccttgcag cgcaggatcg cgtagagcgc gacattcagc agcaggccag acaggatcgc
     5521 tgacaccggc gtcgggcctt cggcgtgtgc atccggcagc cagttatgca gcggcaccaa
     5581 acccaccttg gtgccgtagc cgatgaacag aaacgcgaac gccagcgtga tgatggtcgg
     5641 atcgagctgg gccttgaccg catcgagatt ggtccagatc agcgcgccgc cttccggacc
     5701 gaccactttt tcggccgcca tgtagagcag caccgtgccg aacaacgctt gcgcgattcc
     5761 gacaccgcac aggatgaaat atttccacgc ggcctcaagc ctcgccgccg tgcgatacac
     5821 gctcaccagc aacacggtcg tgagcgttgc ggcctccatc gcgacccaga ggatgcccat
     5881 gttgttggtg gtcagcgcaa gcagcatggt gaagctgaag gactggtaca tgctgtggta
     5941 gagccgcagg cggcgtggag tcatcttgcc gtggtcgcgc tccacgcgca tatacggacg
     6001 ggaaaacagc gctgtcgtca atcccacaaa agcggtcagc gtcacgatga acacattcag
     6061 cggatcgatg aaaaacgcgc gattccatgc aaacaccggt cccatggaaa ttacttgaac
     6121 ggtcagtgca caggctgcaa tgaatgtgcc gaaactgaac gcaacattga tctccggcgc
     6181 gaaattgcgg tggccggtga aggcgagaat cacgctgcca agcagtggaa tgccgaagac
     6241 gaagaacaaa atgtccattc agtcttcttt cagcttttcc agatgccgga tgtcgaggct
     6301 gtcaaactgc tcgcggatct ggaacatgaa cacgccaagg atcaggatac cgaccagcac
     6361 gtcgagcgcg atgcccagtt cgaccaccat gggcatgccg taggttgccg cagtcgccgc
     6421 gaaaaacaga ccattttcca tcgacaggaa accgataacc tgcggcaccg ccttggaacg
     6481 cgtgatcatc atcatgaacg acagcaacat gcaggcaagc gcaatgccga gcgttccgcg
     6541 cgcaatcgaa gacgacagtt gcgacaccgg cagcgcgagg taaaacgcca ggatcaccag
     6601 cagaatgccg atcagcatgg tggttggaat gttgatgagc gtctccacat cccagcgcac
     6661 gttcagccga tcgatcaccc ggtgcagtaa ccacggaatc agaaatacct tgagcaccag
     6721 cgtgatgctc gcggaaatat agagatgcgg ctgatgcgtc gcatagccga ccaccgcagt
     6781 ggccgccgcc agggtcgcac cctgcaacgt gaacagataa ataagcgaga gcacgcgccg
     6841 ctgcgaaatc attgcgaacg cgagcagcag cagaacggcg gcgaacaggt tgatcagctg
     6901 cgaagcaatg ccgctcatcg gctcaggcgc ccagcaggat gtggaccagc atggcgatga
     6961 ccgccagcag gaacgccgtg cagagaaact ccggcacgcg gaagatgcgc atcttggcgc
     7021 tcacggtttc cagcacggca agcagaacgc cgccaacagc cagcttcagg atcaataccg
     7081 gcagtgccag caacagggct accggcgcct gggcgtccgc catgccccaa gggatgaaca
     7141 acgcgagacc gatgcaggag tatgcgaaca gtttcaggct cgccgcccat tccaccagcg
     7201 caagatgacg gccggaatat tccaggatca gggcctcgtg gatcatggtc aattcaaggt
     7261 gcgtggcggg attgtccacc ggcacccgtg cattttcggc gagcgacacc atcgtgaatg
     7321 ccacgcccgc aaaggccagg ctgggaaaaa tggccaggtc acgccctcca agcgtgtcga
     7381 cgatactggt gagtgaggtg gaattggata tcagcgcggc cgtaaacagc accatcagca
     7441 gcgcgggttc cgccagaaag ccgatcagca tttcgcgccg cgcaccgaga ttgccaaagg
     7501 ccgtgccgac atccattgca gccagcgaga tgaacacgcg cgcgagcgca aacaggccca
     7561 ccaacgcaat ggcatctgca gccgaggcaa gcggcagatc ggtggacagc atcgggatga
     7621 ttgcgcacgc caaagtcatg catccgaaga tgatgtaagg ggcaatccga aaaaaaggcg
     7681 atgcgtggac ggcaaccacg gaatccttgt tgaacaattt gtgcaacatc cggtactgct
     7741 gcagcaggct gggcgccgac ttgttctgca gccatgcccg ccactggttc acccagcccg
     7801 taagcaatgg cgccagtgca agcgccacaa taatggccag cagctgggaa atcacgccga
     7861 gcggattcat cgcgtcaccc acaccaggag tgcgatcagc gtgacgaagc tgtacagcaa
     7921 atagaccgag atgcggccct gctgcatcag cccgatcagc cgggccatgc gctcagtgac
     7981 gtgggccacg ggcagataca gccagtacca gaaatgatct ccgatgctga cccggtagcg
     8041 cggctgcgca tcgaaaggtg tcggcagttg ccgctcgaca cgaaacaggc tttcgaaaat
     8101 ctggcggatc ggttggccga aaccttctgc cgtgtcctgc atgcgcggcg tcagccaggg
     8161 aaagccgcag tcccagggcg ccgaacggcg caagcgacca tggtagagct tgcggaccag
     8221 caggaaagcc agcgcaaaac tcgcggccac tccggccagg aaaataatcg ggccgtagct
     8281 ggctcgttcg gcagcaaccg gtatcagcag ccagccgttt gcagcaaccg tactgcccag
     8341 tccggcaccc gtgagttgcc gcgtcaccgg gtcgatgaac tggatgaact gcgcgggcag
     8401 caagcccagc aatacacaac cgcacgccag ccagatcatg cccgccctct cccagcggcc
     8461 tgcatcgtgg acgtgcgcca gttgttcctc gcgtggctgt cccagaaaaa taatcccgaa
     8521 gaatttgacc atcgtataac cagcaagtgc ggcaatcagg gcgacaagcg ccgcggagat
     8581 cggaatcagc atgttcagaa aggagctggg taattcgggg gtaaacagaa agccctgcag
     8641 cagcagccat tcggacacga aaccagacag cggcggcaaa ccggcgctgg tcagcacgcc
     8701 gatgagacaa atccatgcca cccacggcat gtagcggatg agaccgccaa gcttgccgag
     8761 gctgcgctgg ctggtggcat gcaagacagc ccccgtgctg acgaacagaa ggcttttgaa
     8821 gaacgcatga ctgacaacat ggtagagcgc tgcagtcagt gcgagcgtgg ccatcggcgt
     8881 catgccgaa
//
LOCUS       DATMZQ010000002         4239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1581, whole genome shotgun sequence.
ACCESSION   DATMZQ010000002 DATMZQ010000000
VERSION     DATMZQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..882)
                     /locus_tag="VN496_00045"
     CDS             complement(103..882)
                     /locus_tag="VN496_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15358.1"
                     /translation="MDLGLKGKTALVCAASKGLGKGCAFSLAREGVNVTILARGREAL
                     EATAEEIRKATGVKVTAVAVDITTEAGRKQALSACPNPDILVNNAGGPPPGDFREFTH
                     DMWLAALEANMLTPIELIKATVDGMISRKFGRVVNITSSAVKAPIDILGLSNGARTGL
                     TGFVAGIARKTVAHNVTINNLLPGMFDTDRLKSSSIAAAKMQGKPVDEVHAARMKTVP
                     AGRFGNPDEFGDACAYLCSKQAGYITGMNFLIDGGAYPGTF"
     gene            1052..1876
                     /locus_tag="VN496_00050"
     CDS             1052..1876
                     /locus_tag="VN496_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HXF15359.1"
                     /translation="MNVRLVVPLLFGMLMLGGCAAGPNPRDPMEPFNRKVYAFNETVD
                     KAVLKPVAKGYVAVVPEIVRRGVRNVFNNLGMVVTTLNDALQLKGTKVPLDFARFTTN
                     MVFGLGGLIDVASELKIENRNEDFGQTLGYWGVPSGPFLTLPLLGASDMRDGPALLVD
                     FFTNPLYYSRADSQIRWGLFAVDVVDTRANLLGAEKFLETAAVDRYSFLRDAYLQRRE
                     YLINDGRPPANTPSQQQQKTLKQLEEEDESDEPPSEPNSQSGPEGPPFTVAPKIRR"
     gene            complement(1951..4005)
                     /locus_tag="VN496_00055"
     CDS             complement(1951..4005)
                     /locus_tag="VN496_00055"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15360.1"
                     /translation="MSAEIAIPLHEQVVTFLERGTGSDHDAYELAACFQALANARNLA
                     HRVDALIALIAWMDAHSRDGQIERFRLIKILEVLESLPAARRGLQDTFAEILSETEGT
                     NLFGETGIPGERGFLAQLLDRVMGRVLPAPNDDHDLARLVARLCASRLQIRSFEAIAP
                     EFFHRLVQAVAPVDRPEIWSPLKKAFIDGFRLLAIRVQAQGLSSKLRARSHAAPVATS
                     PFYLLAQMSERLADAWGDDENTSALVQQWRELCADCRAGIAEIASRLETAGVSVDILH
                     GLEVLECCLKRMDAMLYVMEAPPGQIGSEAIHRLLAALIVAAQDDKSVRHLVGANLQM
                     LQRKIVERQGNSTEPYVAHTRREYNFIWLAAAAGGLLTVLTVAVKLKLSHTGLPLFIE
                     GLAAGLNYAASFILLQHFHLILATKQPAMTAATLANLLRTRDGAERLDSVVEFAVRIC
                     RSQFAAAVANVVAVFAGVFLFNFVWKFSLGHNFLGEKEAQYVFETLSPVTSGTVFYAA
                     LTGVILWAAAMFGAWMDNWTVYHRLPQAIADHRLAERFGRGRMVRAAGIVSRNISGWA
                     TSISLGLLLGFAPVIGKFIGLPLEVRHVTLSSGMLAFADAGLRGWFSTRWLFSVLAWV
                     VILFLLNLGVSFLLSLYTATRACKLERKELVVFAARLLQRLIKRPLDFVIPRGAANTL
                     RA"
BASE COUNT          950 a         1247 c         1191 g          851 t
ORIGIN      
        1 tcacgacatt cacgacattc acgacagtca cgcagtcacg tcgtcacgac agttatgggc
       61 gcagcccggt atccctttag ggacacgcag tcacggcatt cactagaacg ttcccggata
      121 agctcctcca tcgatcagaa agttcatgcc agtgatgtag ccggcctgct tgctgcacag
      181 gtaagcgcaa gcgtcaccga attcatccgg gttgccgaag cgcccagcgg gcacggtttt
      241 catgcgcgcg gcatggactt catcgacggg tttgccctgc attttggccg cggcgatgct
      301 gctgcttttg agccgatcgg tatcgaacat gccgggcaac aggttgttga tggtcacgtt
      361 gtgcgcgact gtcttgcgcg caatgcctgc aacgaaaccg gtcagacccg tgcgtgcgcc
      421 gttggacaaa ccaaggatgt cgatgggcgc cttgactgcg ctcgaagtaa tattcaccac
      481 gcggccgaac ttgcgcgaaa tcattccgtc cactgtcgct ttgataagct caatgggcgt
      541 cagcatgttg gcttccagcg cggcgagcca catgtcgtgc gtaaactcgc ggaaatcgcc
      601 gggtggagga ccaccggcgt tgttgaccag aatgtcagga ttggggcacg cggagagtgc
      661 ctgtttgcgg ccggcttccg tcgtgatatc gaccgcgacg gcagtgacct tcactccggt
      721 ggccttgcga atttcttcag ccgtagcttc cagcgcctcg cggccgcgcg ccagtatggt
      781 tacattgacg ccttcgcgcg ccagcgaaaa cgcgcatccc ttgccgagcc ccttgctcgc
      841 cgcacacacc agcgctgtct tgcctttcaa gccgagatcc atggttgtct tcttccttcg
      901 aggtattttc agtggaatgg cgacacgaaa cggcgtgccg aagggggcta atcgtgaagc
      961 atgctggcag cttttgcaaa ccaccgcacc ccgccgctgt cgaagcggct ggccgggcta
     1021 taatcggcct aactataaca acagaaatta aatgaacgta cgattggtcg tccctctact
     1081 gtttggcatg cttatgctcg gtggatgcgc agcaggtccc aatccgcggg atccgatgga
     1141 accattcaat cgcaaggtct acgccttcaa tgagaccgtg gacaaggcgg tgctcaaacc
     1201 cgtggcaaag ggctacgtcg ccgtagtgcc agagatcgtg cgtcgcggtg tgcgcaatgt
     1261 cttcaacaat ctcggcatgg tggtcaccac gctcaacgat gcgttgcagc tcaagggcac
     1321 aaaagttccc ctggattttg cacgcttcac tacgaacatg gtgttcggtc tcggcggcct
     1381 tattgacgtt gcctcggaac tcaagatcga gaatcgcaac gaggacttcg gccagacatt
     1441 gggctactgg ggcgtaccca gtgggccttt tcttacgttg cctctgctgg gtgcgagcga
     1501 tatgcgggac ggtcccgcct tgctggtcga ttttttcacc aacccgctgt actactcgcg
     1561 cgcggattcg cagatacggt ggggattgtt cgccgtggat gtcgtcgaca cccgcgccaa
     1621 tctcctggga gcggaaaagt ttctcgagac cgcggccgtg gaccgctaca gctttctgcg
     1681 cgatgcttac ctgcaacgcc gggaatattt gattaacgac ggccgtccac ccgcaaacac
     1741 tccatcgcaa caacagcaaa aaaccctgaa gcaactcgag gaagaagacg agagcgacga
     1801 accgccctca gaaccgaact cgcaatccgg gccggaaggc ccgccattca cggtggcgcc
     1861 gaagatccgc agataacaaa caccatccgt cacaagcggc attcactgca gaatgccgcc
     1921 gggccatgtg ccggggccaa tacttgccgg tcaagctctc agcgtattgg cggcaccgcg
     1981 tggaataaca aagtcgagcg gtcgtttgat cagccgctgc aacaatctcg ccgcgaacac
     2041 cactagttct ttgcgctcga gcttgcaggc gcgcgtcgcg gtgtacagcg aaagcaggaa
     2101 actcacgcca agattaagca gaaataggat aaccacccag gcaagcacag aaaaaagcca
     2161 gcgggtgctg aaccagccgc gcaggcctgc atcagcaaac gcgagcatcc cggatgacaa
     2221 ggtgacatgg cgaacttcga gcggcagccc gatgaatttc ccgatgaccg gcgcgaatcc
     2281 gagcaataaa cccaacgaga tgctggttgc ccatcccgaa atattgcgcg aaacaatacc
     2341 cgcggcgcgc accattcggc cacgaccgaa gcgttccgca agacgatggt cggcaatggc
     2401 ctgcggtagc cggtgatata cggtccaatt atccatccat gctccaaaca tggcggcggc
     2461 ccacaggatg acaccggtca gcgcggcgta aaacacggtg ccgctggtga ccggactcaa
     2521 ggtctcgaac acgtactgcg cctctttttc cccgagaaaa ttatggccga gcgaaaactt
     2581 ccagacaaaa ttgaacagga acacgccggc gaaaactgcc actacgtttg caactgccgc
     2641 ggcaaactga gagcggcaaa tacgaactgc aaactcaact acgctatcga ggcgctcagc
     2701 accatcccgc gttctcagga gattcgccag cgtggcggcc gtcatggcag gttgtttagt
     2761 cgccagaatc aaatggaagt gttgcagcag aatgaaactt gcggcgtagt tgagtcccgc
     2821 agccagacct tcgatgaaca gcggcaatcc ggtatgcgag agcttcaatt tcaccgcaac
     2881 cgtcaataca gtcagcagtc caccagctgc tgcggcaagc cagatgaagt tgtattcgcg
     2941 gcgcgtgtgc gccacataag gttcggtgct gttgccttgg cgttcgacga tcttgcgctg
     3001 gagcatttgc agattggcgc cgaccaggtg tcgaacgctt ttgtcatctt gcgccgcgac
     3061 gattaatgcg gccagcagtc gatggatcgc ctcgcttccg atctgacccg gcggagcttc
     3121 catgacgtac agcatcgcgt ccatacgctt caggcagcat tccagcactt cgagtccatg
     3181 caggatgtcc acgctgactc ctgcagtttc cagccgggat gcaatttcag cgattccggc
     3241 gcggcagtcg gcacacaatt ctcgccattg ctgcaccagt gctgatgtgt tttcatcatc
     3301 gccccacgca tccgccaggc gctcactcat ttgcgccagc aggtaaaagg gcgatgtcgc
     3361 tacaggtgct gcgtgactgc gcgcgcgcaa tttcgacgat aacccctggg cctggacccg
     3421 aatcgccagt aaccgaaagc cgtcgatgaa tgccttcttc agcggcgacc atatctcggg
     3481 acgatcgact ggcgccaccg cctgcacgag cctgtgaaaa aattctggcg ctatcgcctc
     3541 gaagcttcgt atctggaggc ggctggcaca gagcctggcg accagccggg caagatcatg
     3601 atcgtcattg ggcgcaggca gtacgcgacc catgacacga tccaacaatt gcgcaaggaa
     3661 gccgcgctca ccggggatcc ccgtttcacc aaacaggttt gtgccttcgg tttcactcag
     3721 gatttccgca aaagtgtcct gcagaccgcg gcgtgcagca ggcaaggact ccagcacttc
     3781 aaggatcttg attagacgaa agcgctcgat ctgcccgtcg cgactatggg catccatcca
     3841 tgcaatgagc gcaattaaag cgtcgacgcg gtgcgcgagg ttgcgggcgt tagccagtgc
     3901 ctggaaacac gccgccaatt cgtaggcatc gtgatccgag ccagtgcctc gctcgaggaa
     3961 ggtcacaacc tgttcgtgga ggggaatggc tatctctgcg ctcatttaga cccgttgaga
     4021 ttttcggtag acggaaaata gcacaagccg tgaatatcgc aaggccggaa aggccgtgac
     4081 tgccgtgaat atcgtgaatg tcgtgaatgt cgtgaatatc gtgaatgcag tgaattcgta
     4141 aatccgcatt caatcatgac cggtgcgcac ggcgcaggag aatccgacca gtcgattaga
     4201 gtttttccga cattcacgat aactgccgtg aatatcgtg
//
LOCUS       DATMZQ010000003         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1728389, whole genome shotgun sequence.
ACCESSION   DATMZQ010000003 DATMZQ010000000
VERSION     DATMZQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728389"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VN496_00060"
     CDS             complement(<1..338)
                     /locus_tag="VN496_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15361.1"
                     /translation="MSARTICSLILLAASFILPAHAHDPSAWGGLFRSRDMGANWFPV
                     DAGLFIGGALGVAIHPRDPAQLLYGTDARLLRSKNGGRDWITEGAATIPGAVFAVAFN
                     ADGSGAMASSG"
     gene            complement(425..1408)
                     /locus_tag="VN496_00065"
     CDS             complement(425..1408)
                     /locus_tag="VN496_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF15362.1"
                     /translation="MKNSLKTAMIAAGLAASALAAPVAQAADVCSVWLVENSRFMGVP
                     FGAFMPDRAFVPGSTNKSNNINTVDLPVNVGVIKCGNELMLYDAGWNQTDYHKMTGTD
                     HWSPLSKQIAQLGFDAKDVKKIIVGHGHWDHAGSLDEFPNAVLYIQKEELRGIEWALN
                     YPHPRISAVNDSPGGCARTPACGYPSKTMDSIYGKVLKGKAVIVDGEAEVIPGVKIHP
                     AFRAHTAGSQLLEVPTAIGKLVFGSDAYSSWEAIRDWNIANPQQTDTVQQFLAYEQCY
                     KITGGYQNCVCAHEPLSYTEGYPLTKNSWVGMNGGRLAEITLAPGEKSRKK"
     gene            complement(1506..>2001)
                     /locus_tag="VN496_00070"
     CDS             complement(1506..>2001)
                     /locus_tag="VN496_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15363.1"
                     /translation="IALMAMAALRETSPTKPQLAIPSTEANHERAPITAEEETYAREL
                     WPVHAQVKQDAVKMTFAGLAYKMGDIKREALKERVAPLTAIFESAVAALDKIQPPASM
                     QALHGEYQEAIQLYRDASMEMIKVAADGRDVHLVEAQKKSSRASDLTLKVGDILWPGE
                     FKPN"
BASE COUNT          421 a          590 c          522 g          468 t
ORIGIN      
        1 ccgctggaag ccatcgcgcc gctgccgtcc gcattgaaag cgaccgcaaa taccgccccc
       61 ggtatggttg ccgcaccttc ggtaatccaa tcgcgcccgc cgtttttcga gcgcaacagg
      121 cgcgcatcgg ttccgtagag aagttgagcg ggatctcgcg gatgaatcgc gaccccaagg
      181 gcgcccccaa tgaataggcc ggcgtcaacc gggaaccagt tcgcgcccat gtcccgggaa
      241 cggaataatc cgccccatgc gctgggatca tgcgcatgcg ccggcaaaat aaaacttgcg
      301 gcaagcaaaa tcagagaaca aatcgttctt gcagacacgc gctttccatc agtaacgaag
      361 aataaaaatg cgaggagccg acattcggct cctcgcatct aagttacaac ttatgccgag
      421 taaattactt cttgcgcgat ttttcaccgg gcgccagcgt aatttccgcc agcctgccac
      481 cattcatgcc gacccaggaa ttcttggtca gcggataacc ctcggtgtac gaaagcggct
      541 cgtgtgcaca gacgcagttt tgatagccac ccgtgatctt gtagcactgc tcgtaggcca
      601 ggaactgttg aaccgtatcc gtttgctgtg gattggcgat gttccagtcg cgtatggctt
      661 cccaggacga gtacgcgtca ctgccgaata ccagcttgcc gattgccgtg ggtacttcga
      721 gcaactgaga gcccgccgta tgagcacgga atgcaggatg aatcttcaca ccagggatca
      781 cttccgcttc gccgtccacg atcactgctt tgcccttgag caccttgcca tagatcgagt
      841 ccatggtctt ggatgggtat ccgcaggccg gcgtgcgagc gcagccgccg ggtgaatcgt
      901 tcactgcgct gatgcgcgga tgcgggtagt taagcgccca ctcgatgccg cgaagttcct
      961 ctttctggat atacaggacc gcattgggga attcatccag cgaaccggcg tgatcccagt
     1021 gaccgtggcc aacgataatc ttcttgacgt ccttcgcgtc aaaaccaagc tgcgcaatct
     1081 gcttggacag cggtgaccaa tggtccgtcc cggtcatttt gtgatagtcg gtctggttcc
     1141 agccggcgtc atataacatg agttcgttgc cgcacttgat cacgccaacg ttaaccggca
     1201 gatcgacggt attgatgtta ttgctcttat tggtgctgcc cggcacgaac gcgcgatcgg
     1261 gcatgaaagc cccgaatgga acgcccatga accgactgtt ttccaccaac catacggaac
     1321 atacatcggc agcctgagcc accggtgccg caagcgcgga tgctgcaaga ccggctgcga
     1381 tcattgctgt ttttagactg ttcttcatct tctaacccct ccttgacgat tggactgctt
     1441 gcttactaca ttcaacctca tttccgttcc gaagcacatc gcgatcaggc ccgatgcttt
     1501 ccgaatcagt tgggcttgaa ttcacctggc caaaggatgt caccgacttt caatgtcaga
     1561 tccgatgccc ttgacgattt tttctgtgcc tccaccagat gcacatcgcg cccgtccgct
     1621 gcaaccttga tcatttccat ggatgcatca cgatacagtt gaatagcctc ctggtattca
     1681 ccgtgcagcg cctgcatcga tgcaggtggt tgtattttat ccagagccgc cacagcagat
     1741 tcaaatatgg cagtcaaagg agcgacacgc tctttcaatg cttctcgctt gatatcgccc
     1801 atcttgtacg ccaggcccgc gaacgtcatt ttgactgcat cctgtttcac ttgcgcatgg
     1861 accggccaca gttcgcgcgc gtaagtctct tcctcagccg taattggcgc acgctcgtga
     1921 ttggcctcgg tactgggtat ggcgagttgt ggtttggtgg gtgacgtttc gcgcaatgct
     1981 gccatggcca tgagcgcgat c
//
LOCUS       DATMZQ010000004         3063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3110152, whole genome shotgun sequence.
ACCESSION   DATMZQ010000004 DATMZQ010000000
VERSION     DATMZQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3110152"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VN496_00075"
     CDS             complement(<1..282)
                     /locus_tag="VN496_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF15364.1"
                     /translation="MISYLVRRILYAIPILIGVNMLTFLLFFVVNSPDDMARMQLGQK
                     RVSDEAVLKWKQERGYDKPRFFNAGANGTGKLTNTIFFEKSVPLFWFNFD"
     gene            complement(293..2458)
                     /locus_tag="VN496_00080"
     CDS             complement(293..2458)
                     /locus_tag="VN496_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF15365.1"
                     /translation="MKGLLLIAALSSAAWLAGCSGAWNNPYPASDSGKNILYSSFSER
                     PKHLDPVQSYAENEAVFTAQIYMPPLQYHYLKRPYELVPLASTDVPHPTFYDAADHPL
                     PETAPGAKVAYSLYEVHIKPGILYQPHPAFAKDANGNYRYHALKREDLKDVYELRDFK
                     YSGTRELVADDYAFEIRRLAHPKLHSPIFGLISEYILGLKDYAAKLQQVAKKSAPDDY
                     LDLMQFPLDGVQVVDKYTYRIKLKGKYPQFVYWLAMPFFAPVPWEAERFYSQPGMSDG
                     KNLSLDWYPVGTGAYMLTTHDPNRMMVLERNPNFAGEAYPSEGEEGDKAAGLLDDAGK
                     MMPFVDKAVFSLEKESIPYWNKFLQGYYDASGITSDSFDQTVQITASGDVTLADEMRK
                     QDIRLQTSVATSIMYMGFNMLDPVVGGDSERTRKLRQAISIAIDQEEFISIFTNGRGI
                     PGQGPIPPGIFGHREGQEGVNPYVYDWVNGKPERKSVKYAKQLLAEAGYPDGMDKSSG
                     KPLVIYFDTTLTGVGGKSRIDWLAKQFQKIDLQMVVRNSDFNRFQDKIRKGTAQLFFF
                     GWNADYPDPENFLFLFYGAQSRTKGGENAANYQNAEFDQMFERMQVMANGPERQALID
                     RMVEMLRHDAPWVYMLYPKDYTLAHAWVHNRKPNKMANNGLKYQRIDAKLREQKRREW
                     NHPVIWPIALLIAALALSIVPAVGSYRRRERASGKLQTA"
     gene            2564..>3063
                     /locus_tag="VN496_00085"
     CDS             2564..>3063
                     /locus_tag="VN496_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15366.1"
                     /translation="MALLQNKNILVTGLLSSRSIAYGIAKAMTREGARLAFSYQGERV
                     KDRVVELAAEFDSKLVLPCDVASDKEIDALFESLKMHWDGLDGIVHSIAFAPREALAG
                     DYLESVSREAFRIAHDVSSYSFAALAKAGLPMMQGRNAALVTLTYLGAERSFPSYNVM
                     GLAKASL"
BASE COUNT          665 a          840 c          837 g          721 t
ORIGIN      
        1 atcaaaattg aaccagaaca atggcaccga cttctcgaag aatatggtat tggtcaattt
       61 gccggtgcca ttcgcgcccg cattgaaaaa ccttggcttg tcgtaacccc tctcctgctt
      121 ccatttcagc acggcctcgt cgctgacccg tttttgcccc aattgcatgc gagccatatc
      181 gtctggggaa ttgacgacga agaaaagcag gaaggtcagc atattcacgc cgatcaggat
      241 cgggatcgca tacagaatgc ggcgcaccag atagctgatc atcgctgcgg tatcacgctg
      301 tttgcagctt gccgctcgcg cgctcgcggc ggcggtatga tcccaccgcg ggcacgatgg
      361 acagcgccag cgctgcaatc aaaagggcaa tcggccagat gaccgggtgg ttccattccc
      421 ggcgtttctg ctcacggagt ttcgcatcga tgcgctggta cttcagcccg ttattggcca
      481 tcttgtttgg cttgcggttg tggacccagg cgtgcgcgag cgtgtagtct tttggataga
      541 gcatgtaaac ccagggcgca tcgtggcgga gcatttcgac catgcggtcg atcagggcct
      601 gccgctcggg gccattggcc atgacctgca tgcgttcgaa catttgatcg aattctgcat
      661 tctgatagtt ggcggcattc tccccgcctt tggtgcggga ctgcgctcca taaaacaaaa
      721 acaggaaatt ttcgggatcg ggatagtccg cattccagcc gaagaaaaat aattgtgcgg
      781 taccttttcg aatcttgtcc tggaaccggt tgaaatcgct gttgcgaacg accatctgca
      841 agtcgatttt ttgaaactgc ttcgccagcc agtcgatgcg ggatttacct cccaccccag
      901 tcaatgtcgt atcgaagtaa atgaccagcg gcttgccgct ggacttgtcc attccgtccg
      961 gatagccggc ttcggccaga agctgctttg cgtacttcac agacttgcgc tccggcttgc
     1021 cgttgaccca gtcatacacg tatggattta cgccttcctg gccctcacga tggccgaata
     1081 tgccgggggg aatcggacct tgtcccggga tacctcgccc gttagtgaat atggaaatga
     1141 attcttcctg atcgattgcg atggagatcg cctggcgcag cttgcgggtt cgctcggaat
     1201 cgcctcccac gacagggtcc agcatattga agcccatgta catgatcgat gtcgccactg
     1261 aagtctgcaa acggatatcc tgcttgcgca tttcatcagc cagggttacg tcaccgctcg
     1321 ccgttatctg aacggtctgg tcaaagctat ccgacgtaat gccagatgca tcgtaataac
     1381 cctgcaagaa cttattccag tacgggatgc tttccttctc cagactgaag accgccttgt
     1441 cgacgaacgg catcatcttg ccggcatcgt caagcaggcc ggccgcttta tcaccctctt
     1501 cgccctctga cggatacgct tccccggcga aattcggatt gcgttccaga accatcatcc
     1561 ggttcgggtc atgcgtggta agcatgtacg ccccggtccc caccggatac caatcaagcg
     1621 atagattttt gccatcactc atgcctggct gcgagtagaa gcgctcagct tcccaaggca
     1681 ccggagcgaa aaaaggcatg gccagccaat aaacgaattg cggatactta cccttgagtt
     1741 ttattcgata agtgtatttg tctacgacct gcacgccatc caatggaaac tgcatgagat
     1801 cgagataatc atccggcgcc gacttctttg ccacctgttg caactttgcg gcgtaatctt
     1861 tcagtcccaa aatatattcg ctgatcaggc cgaagatcgg agagtgcaat ttgggatgcg
     1921 caaggcgcct tatctcaaat gcatagtcgt ctgccacgag ttcccgcgtg ccgctgtact
     1981 tgaagtctcg caattcgtag acgtctttaa ggtcttcgcg cttcagcgcg tggtaacgat
     2041 aatttccgtt tgcgtctttg gcaaaggcgg gatgcggctg atacaagatg ccgggcttga
     2101 tgtgaacctc gtaaagactg taggcaacct tggcgcccgg tgcggtctcc ggcaacgggt
     2161 gatccgccgc gtcgtaaaag gttgggtgcg gcacgtccgt gctcgccagg ggaaccagct
     2221 catatggccg cttgagatag tggtattgca gcgggggcat atagatctgc gccgtaaaca
     2281 ccgcttcgtt ctcggcatag gactgcaccg gatcaagatg cttgggccgt tctgaaaacg
     2341 acgaatagag aatgttcttg ccggagtcac ttgcggggta cggattgttc catgccccgc
     2401 tgcagccggc cagccatgca gccgaggaca acgccgcgat tagtaataga cccttcattt
     2461 cggccaagtg taacggaatc ggggaccgta gttgctcgta tcgctggcat acgagcgcac
     2521 caacgctttc gctataatgc ccgcagcttc gagggaaatc gacatggcat tgctacagaa
     2581 caaaaatatc ctggttactg gtttactcag cagtcgttcc atcgcctacg gtatcgcgaa
     2641 agccatgaca cgcgaaggag cgcggcttgc cttcagctat cagggagagc gggtgaaaga
     2701 ccgtgtagtt gagctcgccg cagagttcga cagcaaattg gtgctgccgt gcgatgttgc
     2761 cagtgacaag gaaatcgatg ctttgttcga gtcgcttaaa atgcattggg acggcctgga
     2821 tgggatcgtg cattcgatag cgtttgctcc gcgcgaagca ctcgccggag actatctgga
     2881 atcagtcagc cgggaagcgt ttcgcattgc acacgacgtc agctcttata gtttcgctgc
     2941 gctcgctaag gccgggttgc ccatgatgca gggacgcaac gccgcgctgg tgacattgac
     3001 ttaccttgga gccgaacggt ctttcccaag ctacaacgta atggggctgg caaaggcaag
     3061 cct
//
LOCUS       DATMZQ010000005         5276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2419811, whole genome shotgun sequence.
ACCESSION   DATMZQ010000005 DATMZQ010000000
VERSION     DATMZQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419811"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            649..2247
                     /locus_tag="VN496_00090"
     CDS             649..2247
                     /locus_tag="VN496_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXF15367.1"
                     /translation="MSLRALLLVGVICCLRANAALAAPAIHYTDLDSAPVNAWVTIYG
                     SGFGTSQGSGAVYLGGSAALSVVTWSDNKIEAKLPASTGSGLTVVTSSGTSNSMTFTA
                     RTTGRILFADPVNGNNSWDGTAATFQGGSKGPWKDQGSNQNNSNPNAVSAGFYALNPG
                     DVLYLRGGSYAFATNTYGGKTWLFLNGERGGVSNPDVGSAGLPIAMVSYPGELAEGGS
                     DSFGVGIMPYGNIHDITIAKIRLRSGSAAMSMNETQNARLRFVDIETAGPMNDQYNAF
                     GFVGTDGLKVLGNKIHDVGKAGEKLSHAIYYEGYGTSTDVEIAYNTIVNERGGRGIQV
                     YAHTATDRLDVLSIHDNYIDTTTLTGILVGQGDGGAVSNWVRKANIFNNVVRRSGNPN
                     GYSSNGYLPEQAGIHLTLSGCDFVVTHNTLVGDNVYRTLWLEASKTAVIRDNLFDTKA
                     VVVGSGAAGVTWDHNGFAGGLSAPSGAAASVVAASAGYVGGENFSLAASSPFKGKASD
                     GSDIGVVSSVLAVKRPAALQITVH"
     gene            complement(2297..3958)
                     /locus_tag="VN496_00095"
     CDS             complement(2297..3958)
                     /locus_tag="VN496_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15368.1"
                     /translation="MNFFGVQMRSPGQLLFWLSALLIALAMGQVVDPVFLCTDAAHHI
                     TAAQNILDGRGYSTAVPYYAEQLAFGYIPSPQTVWPPLTSLLLVPLIVIGIPATWSPF
                     VLALIFHIASGWLLIKLCLRLRLSELASYSIGIGWLFYCWPLFTVAIGLSEPMYCTFA
                     IGSLLQLVQATEPSARVVRNLWMSAVLALLAFLSRYLGLAIIAAHGAAALAVVYARDG
                     DRRIAKHVVLTVGFMLAGVLLIFGRNALLVGGIRGGPDAVFAFPIADILQSLRWAGMD
                     VFGAKGPLWVTIPLSLAALLLIAPAVVRALRLCLGTLTGRLLSADAMVAINALVVSFA
                     TLGILLIISRGVNPKDLQARYLMPLVPYWATLIWKSLVELRSVDRIHWARTLAIAVGI
                     FYLLVQLSVWSRYEDAFPWSERKHLRATLNTPYQAGTVAEFLRTNSSDSHPLMGADCQ
                     LLGAVLERPCISLPSATYTTAQYDEKATLAMVRQYCVHLIVPNRLNLDARTPYPDTNK
                     IFFIALERGYVPRWLQPVTLTPGFTVYRVTGSDAECNSKRPTDSQ"
     gene            complement(3955..5010)
                     /locus_tag="VN496_00100"
     CDS             complement(3955..5010)
                     /locus_tag="VN496_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXF15369.1"
                     /translation="MNSDLAGKPLVTLLIPAFNEAATLPTTIPQIDSALVELRGSYDF
                     EAVIVDDGSSDETATVAAALAARHHWIRLIRHPGNRGLSEALRTGFANSRGEYVVVLD
                     ADMSYGAEHIGALLAAIVKHSAHIVIASPYMTGGKITNIPHARYVLSRLANRFLSYVS
                     NSSLSTLTSMVRAYDGPFIRAMVLRGSRMALMPEVIYKAMILRAKVVEIPAHLDWSLL
                     VGPNVRRRSSMRILPQIFSTVMSGFAFRPFMFFILPGLFLGAFAAWVDFWTVAHFVEA
                     YRALPASATDAVTTAVAQAYAHYPYTFIVGLLTTMLSVQLLALGILALQAKRYFEEIF
                     TLGIHLNRRIIELAETE"
BASE COUNT         1102 a         1539 c         1626 g         1009 t
ORIGIN      
        1 cggtcgccag gcccagcacg ccgtagtcgg attgagggag catgcgcgtc aggatcggga
       61 agctgatcag gcccagtagt accgataacc cttggccgcc gagaaaatgc agaaagctgg
      121 tagcgagtcg cctgcccgcg gacgtcggtg cgctcatgcc tggactggcg aatgcatcac
      181 aaattcacga ttttttcgcg acggttttgc gttaccggaa ccgcagaatt ggtcgaagag
      241 gcggtcactt atgttgtgtg tttgctggaa atcggcggtg taactatccg gcgacagttg
      301 aataggccca tttccgacga gtagtctgtt ccgcattgac ccgggttcaa ggatcgagat
      361 cgcgggatct tatccgccgg cacgggttgt cgatacattt tcgtgtcttg gtgtcgtcat
      421 ggattcgctg gaagtgtcgc tgcagatcgt ctcggcgccc atctttccag cgatgagatg
      481 cagtagcgct tgatcagcat ccgcgcctgc agcgcaaccg gtgcccaccc ggcatccggt
      541 attcaggttt tcgccatttg acgcactctc aaatctcgag gaaaaaactt cgccatgctt
      601 aatcgaaatg caattgtccg agcgatcagc ccgttgtgga gcagaacttt gagcctgcgg
      661 gcgctgttgc tggtgggtgt catctgctgt ctgcgcgcca atgctgcgct ggctgcaccg
      721 gcaatccact acaccgatct ggattcggcg ccagtcaatg cctgggtgac gatttacggc
      781 agtggcttcg gcacgagcca gggttccggc gcggtctacc tcggtggctc ggcagctttg
      841 tccgtcgtga catggtcgga taacaagatc gaagccaagt tacctgccag caccggcagc
      901 gggctgacgg tggtgaccag cagcggcacc agcaattcca tgacgttcac cgcccgcacc
      961 accggtcgca tcctgtttgc cgatccggtg aacggaaaca attcctggga cggcactgcg
     1021 gccacctttc aaggcggcag caagggcccg tggaaggatc agggcagcaa tcagaacaac
     1081 tcgaatccga acgcggtcag cgcgggcttt tatgcattga atcccggcga cgttctctat
     1141 cttcgcggtg gcagctacgc gttcgccacc aacacctatg gtggcaagac gtggttgttc
     1201 ctcaatggcg agcgcggcgg tgtgagcaat cccgatgtgg gctccgccgg tctgccgatc
     1261 gccatggtct cctacccggg tgaactggcc gagggtgggt cggattcctt cggagtgggc
     1321 atcatgccct acggcaatat ccacgacatc acgattgcca agatccgcct gcgttctggc
     1381 agcgccgcga tgtcgatgaa tgagacccag aatgcgcggc tgcggtttgt ggatatcgaa
     1441 acggccggcc cgatgaatga ccagtacaac gctttcggct tcgtcggcac cgatggtttg
     1501 aaggtcctcg gcaacaagat tcatgacgtg ggcaaagcgg gtgagaagct ctctcatgcg
     1561 atttactacg aaggctatgg caccagcacc gacgtcgaga tcgcctacaa caccatcgtc
     1621 aatgaacgcg gtggacgagg catccaggtg tatgcgcata cggccaccga tcgcctcgat
     1681 gtactttcga ttcacgacaa ctacatcgat accacgacct tgaccggcat cctggtgggc
     1741 cagggcgatg gtggcgcggt gtccaactgg gtgcgcaagg ccaacatctt caacaacgtc
     1801 gtccgccgct ccggcaatcc gaatggctac tcttcgaacg ggtacctgcc ggagcaggcc
     1861 ggcatccact tgacgctgtc gggttgcgat ttcgtcgtta cccacaacac gctggtcggc
     1921 gacaacgtct atcgcacctt gtggctggaa gcctccaaaa cggcggttat ccgcgacaac
     1981 ctgttcgaca ccaaagccgt cgtggtgggt tccggcgccg ccggcgtcac ctgggatcac
     2041 aacgggtttg ctggcggcct gtcggcgccc tcaggtgccg ctgccagtgt ggtggctgca
     2101 tcggctgggt atgttggcgg cgaaaatttc agtctcgccg cgagcagccc gttcaagggc
     2161 aaggccagcg atggcagcga catcggtgtc gtgtcgtcgg tgctggccgt aaagaggcct
     2221 gcggcactgc agattacggt gcactgagtc cgctaacaaa aaggacgaca aacgggccgg
     2281 ccttgccggc ccgttttcat tgggagtccg tgggccgctt gctgttgcac tcggcgtcgc
     2341 taccggtaac gcggtaaacg gtgaaaccgg gagtcagtgt cactggctgt aaccagcggg
     2401 gcacgtatcc tctttcgagt gcgatgaaga aaatcttgtt ggtatcggga tagggcgtcc
     2461 gggcgtcgag attcagcctg ttggggacga tgaggtgtac gcagtactgg cgaaccatgg
     2521 ccagcgtggc tttttcatcg tattgcgccg ttgtgtaagt tgcgcttggc aggctgatgc
     2581 aaggcctttc caggaccgcg cccagcagct gacagtcggc acccatcaga gggtgcgagt
     2641 cactgctgtt ggtgcgcagg aattccgcca ccgtgcctgc ctggtaaggc gtgttgagcg
     2701 tggcgcgcaa gtgtttgcgc tcgctccacg gaaaagcatc ttcgtaccgc gaccagacgc
     2761 tcagctgtac caacagatag aagatgccga ccgcgatcgc cagggtgcgt gcccagtgga
     2821 tgcggtcgac gctgcgcagt tccaccagtg atttccagat cagggtcgcc cagtagggca
     2881 ccagtggcat caggtagcgg gcttgcagat ccttgggatt cacgccgcga ctgatgatca
     2941 acaggattcc cagcgtcgcg aaactcacca ccaatgcgtt gatggcgacc attgcatcgg
     3001 cagataacag ccggccggtg agtgtgccga ggcagagccg aagcgcgcgc actactgccg
     3061 gcgcgatcag aagcaaagcc gcgaggctca gtggaattgt gacccacagc ggccccttgg
     3121 cgccgaacac atccattccc gcccagcgca gtgattgcag aatatcggca atggggaagg
     3181 caaataccgc gtcgggacca ccgcgaattc cacccacgag cagtgcattg cgcccgaaaa
     3241 tcagcagcac tcctgcgagc atgaacccga cggtgagcac cacatgcttg gcaatgcggc
     3301 ggtcgccgtc ccgcgcatac acgacggcga gcgcggcggc gccgtgcgcg gcgatgattg
     3361 ccaggccgag gtagcgactg aggaatgcca gcaatgccag caccgcggac atccacagat
     3421 tcctgaccac gcgagcggat ggttcggtgg cctgcaccag ctgcagcaga ctgccaatcg
     3481 caaacgtgca atacatgggc tcgctcaggc cgatcgccac cgtgaacagc ggccagcagt
     3541 agaacagcca gccaattccg atcgagtaac tggccaattc cgacaatcgc agcctcaggc
     3601 atagcttgat cagcagccaa ccggatgcaa tgtgaaaaat cagcgccagc acgaatggcg
     3661 accaggtagc tggtattcca atgacgatca ggggcaccag cagcagcgag gtcagtggcg
     3721 gccacaccgt ttgcggcgac gggatgtacc cgaatgcgag ctgttcagcg tagtagggta
     3781 cggccgtgga atagccgcgc ccatccagga tgttctgggc ggcggtgatg tgatgagccg
     3841 cgtcggtgca caggaacacc gggtcgacga cctgtcccat ggccagggct atcagcaatg
     3901 cgctgagcca gaacaacagc tggcccgggc tgcgcatctg gacaccgaaa aagttcattc
     3961 ggtctcggcg agttcgatta tccggcgatt gagatggatg ccgagcgtaa agatttcttc
     4021 gaaatagcgc tttgcttgca gtgccaggat gcccaacgcg agcaattgca ccgacagcat
     4081 cgtggtcaac aggccgacga tgaacgtgta cggataatgc gcgtaggctt gagcgactgc
     4141 agtggtcacg gcatcagttg ccgatgccgg cagcgcccgg tacgcctcga cgaagtgcgc
     4201 caccgtccag aagtccaccc acgcggcaaa cgcgccgagg aacaatcccg gcaggatgaa
     4261 gaacatgaac ggtcggaacg cgaatcccga catcacggtg ctgaatatct gcggcaggat
     4321 gcgcatgctg gaacgccgcc gcacattcgg gccgaccagc aacgaccagt cgagatgcgc
     4381 cgggatctcg accaccttgg cgcgcaggat catcgccttg tagatgactt ccggcatcag
     4441 tgccatgcgt gagccacgca ataccatcgc gcgaatgaat ggcccgtcat aggcccggac
     4501 catcgaggtg agcgtcgaca ggctggaatt ggacacgtac gacaggaagc ggttggcaag
     4561 ccggctcagc acgtaacgtg cgtgcgggat gttcgtgatc tttccgcccg tcatataggg
     4621 cgacgcgatc acgatgtgcg ccgaatgttt cacgatggcc gcgagcagcg cgccgatgtg
     4681 ctcagcccca tagctcatgt cggcgtccag cacgaccacg tactcgccgc gcgaattggc
     4741 gaagccggta cgcagcgcct cactcagtcc gcgattgccg ggatgccgga tcaaccttat
     4801 ccaatggtgg cgggccgcaa gagccgcagc gacagtggca gtctcatcgc tgctgccgtc
     4861 atcgacgatc accgcttcaa aatcgtagct gccgcggagt tcaaccagcg cggaatcgat
     4921 ttgcggaatc gtggtgggta gcgtggcggc ttcgttgaac gccggtatca ggagcgtaac
     4981 cagcggcttt ccggcaaggt ctgaattcat gttgttatcg ttgttatggc aaaaattcca
     5041 acatccactc ggttcgactt atcagcccgc tcgccgcaga ccgcagcgta ggatgatggc
     5101 gcatccctga acgccaactc ggcagaaata gtagaagtgt gaattgtatt cgaatgccag
     5161 catgaggatc gtttataggg cctttctgcg gcgtaccgcg tcgggtaccc tcgctcgcga
     5221 tcgcatccac gagtaataag accaacaagg actcggcgat tctcaggccc gggccg
//
LOCUS       DATMZQ010000006         7918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1729300, whole genome shotgun sequence.
ACCESSION   DATMZQ010000006 DATMZQ010000000
VERSION     DATMZQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729300"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1250
                     /locus_tag="VN496_00105"
     CDS             192..1250
                     /locus_tag="VN496_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXF15370.1"
                     /translation="MNTRALEQRPGVMDNSDLVPAPFLSAPGASGIRWNSRSCVACPG
                     LTVIYLERIAESTKEHYSDTAITVLIPGDLGAFKASYRTGDGRRCRTYVRAPELSVIP
                     PGQSHALAVARQSDMLTIAVDCKFFREIARIAIASETTGIEAAEVVGQFAAADPLLRE
                     LGNTLFREFRLNGTPGTAYLECLAGVVAIHLAANYCSGHVLPFYSGLSPHRLKIVQGF
                     IKEHLSEAIRVEQLAAVVHMSPFHFARMFKKATGKAPHAYVNAQRIEHAKELLRESDL
                     PLVDVAATVGFQTQAHFTGVFQKHVSVTPRNFRLKFRASARPGWCEGENDPRHGRNPT
                     SSASFGKKLPDNAANIFP"
     gene            1351..2046
                     /locus_tag="VN496_00110"
     CDS             1351..2046
                     /locus_tag="VN496_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HXF15371.1"
                     /translation="MMHATDQNSSTGIAASSGSAMDDLLSRSWWMLALRGVIAVLFGV
                     LALMWPGITLLWLVLLFAAYALLSGIVSVAGAVTNRKIDKQWWMILLLGLVSIVAGVF
                     AVMNPGLTALALVLVMGANAVVVGVLDIVVAARLRKTRRNEWLLILAGVISVVFGVLV
                     LLFPGAGALALVWLISTYAIFTGILLLALAFRAQVWAKKSRRSAPLSGGQPMTEGSAA
                     TGPAAMGKHHPTT"
     gene            2086..2583
                     /locus_tag="VN496_00115"
     CDS             2086..2583
                     /locus_tag="VN496_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXF15372.1"
                     /translation="MNWKNVCNVIIAALCGIALAGGVVAEDKGKTAEANKTAEAKKDS
                     NAANQNDKDKKEVDAPVAGRVPLGVTVVEADLVAAGYRASKLMHSEVFNDKNQKIGKI
                     DDMVVAPDGSLSLAVIDVGGFLGIASHKVAIPVRQFKQMHPKLVLPGASKEELKKLPE
                     FVYTS"
     gene            2656..3297
                     /locus_tag="VN496_00120"
     CDS             2656..3297
                     /locus_tag="VN496_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXF15373.1"
                     /translation="MEAQTTEKRETNTVTPRQTGTGKKSDAGKKSDVWNPFKFLRKSP
                     EERRSDPSSGMATAGSNLPAAFDSSRFFPTDPLRLMQDLWRDPFSGFGALDRWFGDFS
                     PAVVQPKIDVIDEGDAVRINAELPGIDQKDIEVLIEDDSVILRGEKKMESESEEKGCY
                     RIERSFGSFQRVIPLPDGVDVSRAEASMDKAVLKVRIPKTTSEKSGAQRVEIK"
     gene            3874..4485
                     /locus_tag="VN496_00125"
     CDS             3874..4485
                     /locus_tag="VN496_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HXF15374.1"
                     /translation="MRDLDATVACPSCDLIQRIPPMPTSGTAHCPRCGEGVATRHANT
                     LEQTLALSSAAAILLVIANTAPVIGLSAAGLQASTTLSGAANELWEQGRELSAVVVVF
                     CSIVAPVAYVSFMLTLLLSLRHNPAPAWIGTVMRGISFMAPWSMDEVVMLAILVALIK
                     IASLANTTPGVGMYALGALIVVLAAIKVTFDPREVWRKVRWAA"
     gene            4572..5090
                     /locus_tag="VN496_00130"
     CDS             4572..5090
                     /locus_tag="VN496_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HXF15375.1"
                     /translation="MARARYPLQRTWALVIASAICYIPANTLPVLTTSTLHSSQSDTV
                     LAGIVYLYTSGSWPLALIVLIASVMIPVGKLVALGYLLVSVQRGSVHASRERTRMFRL
                     VEKIGRWSMLDVFVVAFVVALVQLPPLMTAEPGPGVPFFAAVVILTMIAALSFDPRLI
                     WDQVQAQQQAHG"
     gene            5083..6756
                     /locus_tag="VN496_00135"
     CDS             5083..6756
                     /locus_tag="VN496_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXF15376.1"
                     /translation="MADDDRDLEGLPHAIAAPRRHARISMVWIVPIVAALVASGIALE
                     RILSEGPTITIVFDSAQGIEPGKSFIRFKDVNIGQVTAVQLSADYGKVQVTAKIARNA
                     AGLMVEDAQFWVVRPRITLTEISGLNTLLSGNYIGFEAGKAKTPRKHFTGVETPQLLT
                     SGMAGRQFILKARDLGGAGVGSPVYYRRIPVGQVTAYDLASDGNVVDIKVFVNAPYDK
                     YVKRGTRFWNAGGLDVSVGAAGINVRAESIAALLSGSVAFDTPAFAIAEEVAVADAVF
                     TLHRDQLSAMKQPDAITRQYVLRFQESLQGLSVGAPVTFLGLPAGEVTAVGVTLDPGT
                     LDVRPRVAVTLFPERLIATLPIEQQAAAIETSQSVLKSRALVQHLVEARGLRAQLRSA
                     NLLTGQLYVALDYFPAAPKAKPNWDAEAPELPVAPSTLPDLQDKLSSVLAKIDKLPLE
                     ALGTNTTKALESLDRTLKDADRLVNRLDADTVPELKTTLEEARSTLAAASRVMKSAES
                     TLAGADAPAQQELRNTLREVTLAARAVRVLADSLERRPESLVRGRSKTP"
     gene            6753..7337
                     /locus_tag="VN496_00140"
     CDS             6753..7337
                     /locus_tag="VN496_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HXF15377.1"
                     /translation="MTLRQLLSLSALAMLAPGCASTPTSHFYTLNAMAAPGPMPSNVS
                     IVVVCISVPSEVDRPQWVLSTGANEVRLDDFNRWASPLQDAIARVTAENLAALLATPR
                     VSAFPQMVAETDYRVTIEVQRFESRLGDSITFDAVWMLRRTKDGNAELRRTTVREPAP
                     GNSYSELAAAHSRALLRLSTDIAKEITARTSAAT"
BASE COUNT         1743 a         2235 c         2313 g         1627 t
ORIGIN      
        1 gtcccagcct ggacaaacaa atagcagcga cctccggcag taaacacggc acattagaca
       61 ttgtcgccgc gccgcactcg ccattgcgct ggctgtctag tccgctgcgg cccgccagcg
      121 gatcaaaagg gtgtaggcct ctaaaccaag gtgcaatagt tcgcccatcg atcagatcct
      181 attcttgaat tgtgaacacg cgagcactcg aacaaaggcc aggtgtaatg gacaattccg
      241 atctcgtgcc agcgccattt ctttccgcgc ccggcgcatc cggcatccgc tggaattcgc
      301 ggtcgtgtgt agcgtgcccg ggactgaccg tcatttacct ggaacgcatc gcggaatcaa
      361 caaaggaaca ctacagcgat actgctatca cggtcctcat tccaggcgat ctgggcgcct
      421 tcaaagccag ctaccgcaca ggggatggac gtcggtgccg aacgtacgtg cgcgcccccg
      481 agctatcggt gattccgccg ggacagagtc atgcgctggc cgtcgcgcgg caatccgaca
      541 tgctgaccat tgccgtggat tgcaagttct ttcgcgaaat tgcgcgcatt gcaatcgcat
      601 ccgaaaccac tgggatagaa gccgctgagg tggtcgggca atttgccgcg gccgatccac
      661 tcctgagaga actgggtaac acattgttcc gcgaattcag gctgaatggg accccgggca
      721 cggcatacct cgagtgcctg gccggtgtcg tggcgatcca tctggccgca aattattgca
      781 gcggacacgt gttgccgttc tattcgggtt tgtcaccgca tagattgaag atagttcaag
      841 gcttcatcaa ggaacacttg agcgaagcga tcagggtcga acagctggcg gccgttgtac
      901 acatgagtcc cttccatttc gcgcggatgt tcaagaaagc tactggaaaa gcgccgcatg
      961 cctacgtcaa cgcacagaga atagagcacg cgaaggaatt gctgagggag agcgatctgc
     1021 cgctggtgga cgtcgccgca acggtcggct tccagactca ggcacacttc actggtgtat
     1081 ttcaaaagca cgtcagcgtg actccgcgga actttcgact gaaattccgg gccagtgcgc
     1141 gccccgggtg gtgcgaaggg gaaaacgatc cgcgccacgg caggaatcca acatcatccg
     1201 caagcttcgg gaagaagctt cccgataacg cggctaacat tttcccatga tgcgaagcat
     1261 gttccttcca tgagtaaggc aattgtccgg ggaccaaccc ggatgcctaa caaacgatcc
     1321 cgtcatttca ctttccggta gaggccgaac atgatgcatg cgaccgatca aaattcaagc
     1381 accggcattg ccgcatcgag tggcagtgcg atggatgacc ttctttcgcg gtcctggtgg
     1441 atgctggctt tgcgcggagt gatcgccgtg ctattcggcg ttctggcctt gatgtggccc
     1501 ggcattacat tgctgtggct cgtcttgctg ttcgcggctt atgcactgtt gagcgggatc
     1561 gtatcggtcg caggcgccgt gaccaaccgc aaaatagaca aacagtggtg gatgatcttg
     1621 ctgcttgggc tcgttagtat cgtcgcgggt gtcttcgcag tgatgaaccc cggtttgacc
     1681 gcactggcac tcgtactcgt catgggagcc aacgcagtgg tagtcggagt gcttgacatt
     1741 gtcgtcgcag cgcgtttacg caagaccaga cgaaacgagt ggctgctgat cctcgcagga
     1801 gtcatttcag ttgtattcgg agtcctggta ttgctattcc cgggcgccgg agcgctggcc
     1861 ctggtgtggc taatcagcac ttatgccatt ttcacaggaa tcctgttgct ggcactggcc
     1921 ttccgcgcgc aggtctgggc gaagaagtcg aggcgttcgg caccattgtc tggcggccag
     1981 ccgatgactg aaggaagtgc tgcaacaggt cccgcggcta tgggcaaaca ccacccgaca
     2041 acataaaaca gtaacttgaa acgcaacgtc tgaaaggagc aaatcatgaa ctggaaaaac
     2101 gtttgtaacg tcatcatcgc tgccctgtgc ggcatcgcac tggcaggcgg cgtggttgcg
     2161 gaagacaagg gcaagaccgc tgaagccaac aagaccgctg aagccaagaa ggacagcaat
     2221 gccgcgaatc agaacgacaa agacaaaaaa gaggttgacg caccggtagc cggacgggtc
     2281 ccgctcggtg tcacagtggt ggaggccgat ctggtggccg ccggctatcg cgcatcgaag
     2341 ctcatgcatt cagaagtctt caacgacaag aaccagaaga ttggcaagat cgacgacatg
     2401 gtcgtcgcgc cggacggatc gctatccctt gcggtgatcg atgtcggcgg tttcctcgga
     2461 atcgccagcc acaaggtcgc tattccggtg aggcaattca aacagatgca tcccaagctg
     2521 gtgctgcccg gcgcttccaa agaggaactg aagaaacttc ccgagttcgt ttacacgagt
     2581 tgagttgcgc cgcatacacc agccgctgtt tagcagtagg gcgaaggccc tcaacaacaa
     2641 ttccaggaga aatcgatgga agcacaaacc accgaaaagc gcgaaacgaa cacagtgacc
     2701 ccccgtcaaa ccgggaccgg taaaaaatcg gacgccggca agaaatccga tgtctggaat
     2761 ccgttcaaat ttcttcgcaa gtccccggag gaacggcgca gcgatccttc gtcgggtatg
     2821 gcaacggccg gcagcaattt gcccgcagca tttgattcat cgcgattttt tccgaccgat
     2881 ccgttgcgcc tgatgcagga tttgtggcgc gatccgtttt ccggcttcgg tgcgctggat
     2941 cgctggttcg gcgacttcag cccggcagtg gttcagccga aaattgatgt aatcgacgag
     3001 ggcgatgcgg ttcgaatcaa tgccgaactg cccggaatcg atcagaagga catcgaagtt
     3061 cttattgaag acgacagcgt catcctgcgc ggcgaaaaga aaatggagtc agaatcggaa
     3121 gaaaagggat gctaccgcat agagcgttcc ttcggcagtt tccagcgcgt tattccgttg
     3181 cctgatgggg tcgatgtcag ccgtgcggag gcaagcatgg acaaggcggt gctgaaggtg
     3241 cgcatcccca agactacgtc cgaaaaatcg ggggctcagc gggtggaaat caaatagacg
     3301 ggaatggttg aggccggctc gcggctatcg ccatgagccg gcgccattcc tgacgcttca
     3361 cgccagactc ttcctcagtc cgggcactgc gcaaagtgcg cataaacaaa ttggcgcacg
     3421 cgttttctaa aattgcgtag atcctatcgt ccattctttt cttcgcgtcg agctgcccga
     3481 agtgttggca gaattccgaa ctcggtgcgc cgtcaatttc gcgtttcggc cggagacggg
     3541 gtcgcataag actttacacc gcgcaaatca aaaaactgca aagcttccat cggtacgagg
     3601 cgaagcgcga gggatagcgc attcaaacac ccgaaggcac aaacgctgct gtcccgggat
     3661 cgcgaccaac ttgcgtctat ccactggact gttagtaagg ccactgcaaa aattaacttt
     3721 cgcccagtcc ccaaccgcgc aatgtcgaac taaccaggca tccacgtatc tccattaatt
     3781 tcatccggct taattgggcg ggtattgccc gatcggtgct agacttttgc agcacttacc
     3841 ataccgccat gggcctgcag atgagggaaa gtcatgcgcg atcttgatgc aaccgtggca
     3901 tgtccgagtt gcgacctgat ccaacgcatc ccgccaatgc ctacgagcgg gacagcacat
     3961 tgtccaagat gcggtgaagg tgtcgcaacc cgtcacgcca atacgctcga gcaaacgctg
     4021 gcgttaagct cagccgcggc aattctactg gtaattgcaa acactgcgcc ggtgattggg
     4081 ctgtccgccg caggacttca ggcgagcacc acattgagcg gcgcggcgaa tgaattatgg
     4141 gaacagggcc gcgaactgag cgctgtggtg gtggtgttct gcagcatagt cgctccggtg
     4201 gcctatgtca gtttcatgct gacgctattg ctttctcttc gacacaaccc ggcacctgcg
     4261 tggataggca cggttatgcg cggcatcagt ttcatggcac cgtggtcgat ggacgaagtg
     4321 gtgatgctgg cgattctcgt cgcgcttatc aagatagcat cgcttgcgaa taccacaccg
     4381 ggcgtcggga tgtatgcgct gggggcgctg attgtcgtgc tggctgcgat caaagtcacc
     4441 ttcgatccgc gcgaagtctg gcgaaaagtg cggtgggcgg catgatcgtt tcctgcccga
     4501 catgccagtt gttgtcccga gtcccatcaa ggactcatct ggcgcactgc ccgcgttgcg
     4561 ccgcgcggct gatggcgcgc gctcgctacc ccctgcagcg cacctgggct ttggtgattg
     4621 catcagcgat ctgttatatc cccgccaata cattacctgt cctgaccacg agcacgctgc
     4681 attcttcgca gtccgacaca gtgctcgcgg gcatagtcta tctttataca tcggggtcat
     4741 ggccgcttgc gctcatcgtg ctgattgcga gcgtgatgat tccggtgggc aaactcgtgg
     4801 cgctgggata cctgctcgtc agcgttcaac gcggctccgt ccacgccagc cgcgaacgca
     4861 cgcgcatgtt ccgtctcgtc gagaaaatcg gccgctggtc gatgctcgat gtgtttgtcg
     4921 tcgctttcgt cgtagcattg gtgcagctgc cgccgctcat gacggcggaa cccgggccgg
     4981 gcgttccttt ctttgcggcc gtggtgatac tcacgatgat tgccgctttg tcattcgacc
     5041 cccgcttgat ctgggatcaa gtacaggcac agcagcaagc gcatggctga cgacgacaga
     5101 gatctggaag gtctgccgca cgcgatcgca gcgccgcgcc ggcatgcgcg catctcgatg
     5161 gtctggatcg tcccaatcgt tgcggccctc gtggcctccg gtatcgcgct cgaacgcata
     5221 ctgagcgagg ggccaaccat cacaattgtc ttcgattcgg cgcaaggaat agagcccggc
     5281 aaaagtttta tccgcttcaa ggatgtgaac atcgggcagg taacggcggt gcagttgtct
     5341 gccgactatg gcaaagtcca agtcaccgca aaaatcgcca ggaacgcggc gggactgatg
     5401 gtcgaggatg cgcaattctg ggtagtgcga ccgcgcatca cgctgactga aatatcggga
     5461 ctcaatacgc tgctgtcggg caactatatt ggcttcgaag caggcaaggc caagaccccc
     5521 cggaaacatt tcacgggagt cgaaacaccc caattgctca ccagcgggat ggccggtcgg
     5581 caatttatcc tgaaggcccg cgatctgggg ggagcaggtg tgggctcgcc ggtttattac
     5641 cgtcgtattc cagtagggca ggtcactgcc tacgatctgg catccgatgg caatgtagtc
     5701 gacatcaaag tattcgtgaa cgcgccgtac gataaatatg tgaaacgcgg aacacgtttc
     5761 tggaatgccg gggggctgga cgtctcggtt ggggccgctg gtatcaatgt acgcgccgag
     5821 tccatcgcag cgttactctc tggcagtgtc gctttcgata cccctgcgtt tgcaatcgca
     5881 gaggaggtcg cggttgcgga tgccgtcttc accttgcatc gcgatcagct ttccgcgatg
     5941 aagcaacctg atgcaattac caggcagtat gttttacgct tccaggaatc tttgcaaggc
     6001 ttgtcggtgg gcgcgcccgt cacgttcctc ggcctgcccg cgggtgaagt tacggccgta
     6061 ggcgtgacgc tcgatccggg gacgcttgat gtccggccca gagtcgccgt tacgttgttc
     6121 cccgaacggc tcatagcgac tttgcccatc gagcagcagg cagccgccat cgagaccagc
     6181 caaagcgtgc tcaagagtcg cgccctggtg caacaccttg tcgaggcgcg cggcttgcgc
     6241 gctcagctca gaagcgcaaa tctgctcact ggtcagttgt atgtcgctct tgattatttt
     6301 ccggcagcac cgaaggcaaa accaaactgg gatgccgagg ctccggagct tcccgtagcg
     6361 cccagtacac tgcctgacct gcaggacaaa ctgtccagcg tgctggcgaa gatagacaaa
     6421 ctgccactcg aagcgcttgg cactaatacg accaaagcgc tggagtctct cgaccggacg
     6481 ctgaaggatg cggacaggct ggtgaatcgc ctggatgcag acacggtacc ggaactcaaa
     6541 acaacgctcg aagaagcgcg gagcactctg gctgctgcga gccgtgtcat gaagagtgcg
     6601 gaaagtacgc tggcgggtgc cgatgcgcct gcacagcagg aattgcgcaa cacactgcgg
     6661 gaagtaacct tggctgcgcg cgcggttcgg gtcctggctg actccctgga gcgtcgtcct
     6721 gagtcgctgg ttcgaggccg gtcaaagacc ccatgacact aagacaactg ttatcgctgt
     6781 ctgcgctcgc catgcttgcg cccggctgcg catcgacacc tacttcgcat ttttacacgc
     6841 tgaacgccat ggccgctccc ggtcccatgc cctctaacgt ttctatcgtg gtggtgtgca
     6901 tctccgtacc ttccgaggtg gaccgcccgc aatgggtctt gagcaccggc gccaacgaag
     6961 tgcggctcga tgatttcaac cgctgggcat cacccctgca ggatgccatt gcgcgcgtga
     7021 cggccgagaa cctcgcagcg ttgctcgcga caccgcgcgt tagcgcattt ccgcagatgg
     7081 ttgcggaaac ggattaccgc gttacgatcg aagtccagcg tttcgaatcg agacttgggg
     7141 attcgatcac gttcgacgcg gtctggatgc tgcggcgtac gaaagacggc aatgccgagc
     7201 tgcgacgtac cacggtacga gaaccggccc ctggcaatag ttatagtgaa ctggcggctg
     7261 cacacagccg ggcactcttg cgcttgagca cggacatcgc gaaggagata acggctcgta
     7321 cttcggctgc cacatagctc tcaatcagga gcatcggaat gagggtgcag gcgaaagtga
     7381 cggatatcaa gcaaggaaat gtcgagcctt cggcattgcg ttaagcccgt tgaaacataa
     7441 tgagtgacgt gcgcgtactt cactaggtga gcaatgtttc atagcagcgc gaatacaggc
     7501 tgcgcaatct acctgccgga caagaaggga agtacacccc ctcagcattg caattacctg
     7561 ctgggaacaa aaagagaatc aaatgccctt gcgggacgct ggttttggct ttcaccctgg
     7621 tcaaacctgt agacaatgac tcgaccgctt gcgctagtcg cgtgcccagc gcgccagaga
     7681 cgttcttgcg gcaccgggtt cgcctacata tgtcactgtg ctaaatacca cataagtagg
     7741 atactgcgag actgttggca ccgacctgac tgcctgaaga ggaaagaaca acaacaatta
     7801 cgggcggggc agcggcagat acaaaaacgc ccggcgcgac cgggaaagag caatgaatcg
     7861 ccggcaattc gatacgcaag cgcaacaggg gaggatttca tatggctgat ctgacggg
//
LOCUS       DATMZQ010000007        28602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1730352, whole genome shotgun sequence.
ACCESSION   DATMZQ010000007 DATMZQ010000000
VERSION     DATMZQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 28602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730352"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VN496_00145"
     CDS             <1..645
                     /locus_tag="VN496_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXF15378.1"
                     /translation="SFKDAFWGLMLVVIVMGGIYGGIFTPTEAAAVSAVYAFVAAVFI
                     YRGLSLRQVPKVLLSAANTSAMLLYIITNAILFSFFLTSEQIPQALSQWVVDQHLSAW
                     TFLIAVNIALLVAGQFMEPSSIILILAPLLVPIARNLGVDAIHLGVIMTVNMEIGMIH
                     PPVGLNLFVASHIAKMGLTEVSIACLPWVVVLLAYLVLITYLPGVSLWLPNLLY"
     gene            complement(656..1198)
                     /locus_tag="VN496_00150"
     CDS             complement(656..1198)
                     /locus_tag="VN496_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXF15379.1"
                     /translation="MPPDPNQPDVLGRPAKSPNGRRLIIAVALIGVSILALALVKHLP
                     GIPAGIGTAPHEPAQALITTPVPGSETPMSKITQPEPAPPKILNSETLVVPPRAAGTA
                     PRAGALPAAPAKTYIVQAGVFNSLANALALQKQLQQAGIRTHLETRVQMGPFRNKHDA
                     DRAIARAKKLGIDAVVVGPR"
     gene            1388..1813
                     /gene="ndk"
                     /locus_tag="VN496_00155"
     CDS             1388..1813
                     /gene="ndk"
                     /locus_tag="VN496_00155"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXF15380.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGQIYSRFEQSGLKIVAAKMMQLSR
                     GDAEGFYSVHRQRPFFNDLVNFMISGPVMVQVLEGESAIQKNRDLMGATDPKKASKGS
                     IRADFADSIDANAVHGSDAPQTAAVEIAYFFPAFNIYSR"
     gene            1813..2898
                     /gene="rlmN"
                     /locus_tag="VN496_00160"
     CDS             1813..2898
                     /gene="rlmN"
                     /locus_tag="VN496_00160"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXF15381.1"
                     /translation="MPVNLLEFDAQGLAAFFAEIGEKPFRAKQVMRWVHQFGQSEFEG
                     MSDLSKDLRAKLKTGATVQSPCLLDDSTASDGTRKWLLGVGAANGIETVFIPETGRGT
                     LCISSQVGCALECAFCSTGRQGFNRNLTVAEIIGQLWWANRALGADRQGERIISNVVM
                     MGMGEPLANFENVVSAMRIMLDDNGYGLSRRRVTVSTSGMVPAMDRLAAQLPVALAVS
                     LHAPDDALRNRLVPINKKYPLKELMAACKRYIKVAPRDFVTFEYVMLDGVNDSVDQAR
                     QLVALVKGVSCKFNLIPFNPFPDSGFARSSRDRIAAFRNVLMEAGLVTTTRKTRGEDI
                     DGACGQLAGRVQDRTQRKSRRTMVAPA"
     gene            2895..3659
                     /gene="pilW"
                     /locus_tag="VN496_00165"
     CDS             2895..3659
                     /gene="pilW"
                     /locus_tag="VN496_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HXF15382.1"
                     /translation="MKVVFFLILTVAVLAGCTTHPTKSETGEPRNAAENRARIHTELG
                     AAYYGIGQMPVAIQELKEAISADPNYAPAHSQLGLVYMSLKEDALAQQSFERALKIDP
                     SDSSANNNYGMFLCQRKREKEAMKYFAAALKNPLYGSPENAYTNAGICLRMQGDDAKA
                     EEWLRKALALQPDQSLALYQLADISFKRNELIDARTLLNRHMQVSTPSAEALWLGARI
                     EQRLGNRTALASYGAQLNNRYPGALQTRSFNEGKFQ"
     gene            3656..4633
                     /locus_tag="VN496_00170"
     CDS             3656..4633
                     /locus_tag="VN496_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HXF15383.1"
                     /translation="MSGTGMMNEPAPTAAPSPTPGDPGAAGATLLKERRRQGLSLGDI
                     SRQLKLSVRQVEALERDDYSAYKGPVFIHGFIRNYAKLLGLDPEPLIRAADALLKPPS
                     VASADSTTQPEVLRAPGKTGKSLPWGLIGLALFVGIAISVYFGGRRAPDAGRNAPTAV
                     VRDNLNSTKSHPETRSGGETSQPKPRVEVTKPTPKSAAKADARAEPKARSESPTPSVE
                     TRVPAETSVPAETSVPAETGGPIAVDESERLTVRMIFEQDSWVEIKDRAGNTIFGQLN
                     SAGTRRSVSGERPLTVVVGNAAGVRLFQGDKSIDLAPYTRVDVARLTLE"
     gene            4646..5917
                     /gene="ispG"
                     /locus_tag="VN496_00175"
                     /gene_synonym="gcpE"
     CDS             4646..5917
                     /gene="ispG"
                     /locus_tag="VN496_00175"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXF15384.1"
                     /translation="MSGNFVLRHQTRLVRIAGIPVGGGAPVVVQSMTNTDTADASATA
                     QQVRTLAEAGSELVRITVNSAEAAAAVPQIRNILERDRCRVPLIGDFHFNGHKLLKQF
                     PECAQVLDKYRINPGNVGRGSKRDEQFATMIETACRYGKPVRIGVNWGSLDPDLLGRM
                     MDDNARLSEPQEAAAVMREALIVSALQNALKAEEIGLPGDRIVLSCKVSGVQDLIAVY
                     RDLAARCDYPLHLGLTEAGMGSKGIVASTAALAVLLQEGIGDTIRISLTPDPGGDRSR
                     EVVVAQEILQTMGLRSFTPMVIACPGCGRTTSTVFQELAEKIQAHLRQQMPVWRVKYP
                     GVEDMRVAVMGCVVNGPGESKHANIGISLPGSGETPVAPVFVDGIKTVTLKGEHIAEE
                     FHRLVDAYVASRFGRNPSAAANSDESARALG"
     gene            5949..7208
                     /gene="hisS"
                     /locus_tag="VN496_00180"
     CDS             5949..7208
                     /gene="hisS"
                     /locus_tag="VN496_00180"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXF15385.1"
                     /translation="MAQTIRAVRGMNDALPDQTPLWEFFEDTVRAWLAQYGYRNIRMP
                     ILEKTELFVRSIGEVTDIVEKEMYTFVDQLNGESLTLRPEGTASCVRAVIEHNLLYSG
                     AQRLFYMGPMFRHERPQQGRYRQFHQVGVEALGFPGPDVDVEHMAMGADLWRRLGLEG
                     LQLEINTLGNTTSRGLHRAELIAYLERHAASLDDDARRRLHSNPLRVLDSKNPALQDV
                     IAAAPRLADYLDDASRAYFDALQGLLKNAGIDFVVNGRLVRGLDYYNDVVYEWTTSRL
                     GAQGTVCAGGRYDSLIAQIGGKAAPACGFAMGIERVLALLEPARIPARAADVYVVHSG
                     EAASPFAWNVAALLRRRGLAVVFHCGGGSFKSQMKRADASGALFALIVGDDEARKQIV
                     SLKPMRKAGEQVQADPEQAATLIINNR"
     gene            7234..7869
                     /locus_tag="VN496_00185"
     CDS             7234..7869
                     /locus_tag="VN496_00185"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF15386.1"
                     /translation="MAFDLEEQEQIAELKQFWKQYGKLIVTVAVAALLAFAAMQGWRY
                     YKNIESQQASALFTKFAEAVRKNDVKDIRELGQQVMDKFGSTAYGPTAALLLAKTNFE
                     NGEPSAAAGELQWAIDHAKDVETAELARLRLASIRLDEKKYEEALKLLETKHSPAMET
                     LYADLRGDVMLAQGKPAEARAAYKAAIDKSLPNSTYRSVVQIKLDALGSNK"
     gene            7875..8996
                     /gene="bamB"
                     /locus_tag="VN496_00190"
     CDS             7875..8996
                     /gene="bamB"
                     /locus_tag="VN496_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HXF15387.1"
                     /translation="MKLRLAIAFLFVTLLGACASKGPEPARLVEFKPSAKLKVAWKIN
                     VGEAGYYIFTPAIYGDSVFAASNRGDLLRLNASNGKTIWRVDTKAPLSGGVGIGENMV
                     LVGTAKGSVLAYDLQGKPLWQARISSEVLSAPQAAGEFVIVRSGDSRIFGLDARNGAR
                     RWEYQTTPPPLTLRANPGVMVIENFVIAGMPAGKLIVLNIANGGLVWETVVAAPKGDN
                     ELERITDIAGPPLVEAGRVCAATFQGRAACYETQKGGQLWARPASSIGSLATDDLSVY
                     LSEDTGSVVALDRNSGASVWKQDKLAYRNVSAPLATKDYIVVGDFEGQVHFLKFEDGS
                     FAARLPTDGGGIATAPKLLGDKVLVQTRNGGLFAIAFAK"
     gene            9051..10388
                     /gene="der"
                     /locus_tag="VN496_00195"
     CDS             9051..10388
                     /gene="der"
                     /locus_tag="VN496_00195"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXF15388.1"
                     /translation="MNPTIVLVGRPNVGKSTLFNRMTRSRQALVADIPGLTRDRHYGH
                     GEAGGKSYLVVDTGGFEPVAKDGIYKEMARQTRQAVDEADAVLLLVDARQGLTPLDRQ
                     IAAELRKTARKLLLVVNKAEGLAHETAAAEFHELGLGEPLTISASHGEGVRDLVDFVL
                     EEFNPDDAATPPQAQHPRIAVVGRPNVGKSTLVNTVLGEERVIAYDKPGTTRDSISID
                     FERNGRFYTLIDTAGVRRRGSVEDVAEKYSVVKTMQAIEDCNVVMLVLDVTQEISDQD
                     AHLAGFILEAGRALVVAINKWDAANAEAREKAKRDYGRKLNFLDFAAVHYISARTGTG
                     VSALLNSANAAYAAAMSKLPTPKLTRVLLAAVQKQSPPRSGVSRPKLRYAHQGGMNPP
                     LIMIHGNGLSQVPDSYRRYLERTFMAAFELRGTPVKVLFKHQTRNPFAGRSRN"
     gene            10473..10712
                     /gene="hfq"
                     /locus_tag="VN496_00200"
     CDS             10473..10712
                     /gene="hfq"
                     /locus_tag="VN496_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXF15389.1"
                     /translation="MSAKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVNLSLEHGSED"
     gene            10787..11950
                     /gene="hflX"
                     /locus_tag="VN496_00205"
     CDS             10787..11950
                     /gene="hflX"
                     /locus_tag="VN496_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXF15390.1"
                     /translation="MNSRVARALFPDSGSTSDRPWGSDRAVLVSLDFGKLDFTESQEE
                     IRRLAESAGVQPVGAIKGKRQRPDAATFAGSGKVEEIATAVQSNAASLVLFNHDLSPA
                     QQRNLERRFECRVIDRSSLILDIFAQRARSHEGKLQVELAQLEHLSTRLVRGWTHLER
                     QKGGIGLRGPGETQLETDRQLLRKRVKVLKDKLDTVHRQRQTRRRARQRGQALSVSLV
                     GYTNAGKSTLFNRLTHGDAYAADQLFATLDTTSRRISGAHGSAIIMSDTVGFIRDLPH
                     TLVAAFSATLEETVQADLLLHVVDAASPARDDQISEVNKVLAEINADCVPQVVVLNKI
                     DAAGLQPGVERDQYGKISSIRVSATTGAGIGYIRAMLSELHEMQRNADGIVAA"
     gene            12005..13189
                     /gene="hflK"
                     /locus_tag="VN496_00210"
     CDS             12005..13189
                     /gene="hflK"
                     /locus_tag="VN496_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXF15391.1"
                     /translation="MALNDPQWGKRGNDGPPDLDELWRRVNQRLNSLFGGKGGGGNTP
                     SAPGGRQFGSGFGLLVILVVIVWLASGFYIVDASQRGVVLRFGKFVESTLPGPRWHFP
                     WPMESAEVVNVTGVRTVEIGYRNNVKSKVQNESLMITDDENIIDIQFAVQYILKDPQD
                     YLFNNRDADKSVMQAAESAIREVVGKSKMDFLLSGGKEKIVETVQILLQKILDRYKTG
                     ISVMRVTMQDAQAPEEVQAAFADAVKAGQDRERQINEGQSYFNDVVPKAKGTSARLLQ
                     EGEGYRQSVIANAEGEASRFNQIVTEYSKAPVVTRQRLYLDAMQQVLSNSSKVVVDQK
                     AGNNLLYLPLDKIMQLSATSGASADAANKSSPESVSPTPAPAEVSPRSRDAFRNRERE
                     PR"
     gene            13186..14064
                     /gene="hflC"
                     /locus_tag="VN496_00215"
     CDS             13186..14064
                     /gene="hflC"
                     /locus_tag="VN496_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXF15392.1"
                     /translation="MKFNISTVLIAIVIVLVIASLSLFTVDQRQNAIVFRLGEIVAVK
                     TVPGLFFKVPLVDNVRYFDSRILTLDAEEAQRFVTAENKPVLVDSFVKWRIIDVKQYY
                     VSVQGDENLARVRLTQAVNGSLREEFGKRTIHEVVSGEREKIMDLMREKADQTSREIG
                     VQVLDVRLKRVDFTPEISESVYGRMQAERKRVANELRSTGFAEAEKIRADADKQRQVV
                     IAQAYRDAQRLKGDGDAKASAIYARAFERNPEFYAFYRSLEAYRQSFKNKSDLMILEP
                     NSEFFKYMKNPAGHAK"
     gene            14137..14307
                     /locus_tag="VN496_00220"
     CDS             14137..14307
                     /locus_tag="VN496_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HXF15393.1"
                     /translation="MTAMALMLVIEGLLPFLVPKVWRDAFRRLTELSDGQVRFIGLSS
                     MLAGLLLLFLTK"
     gene            14370..15560
                     /locus_tag="VN496_00225"
     CDS             14370..15560
                     /locus_tag="VN496_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXF15394.1"
                     /translation="MQKWLLPEYIEDLLPREAARVESLRRRILDLFFVHGYELVVPPL
                     LEYLESLLTGTGHDLDLRTFKLVDQLSGRLLGLRADMTPQVARIDAHLLNRKGVTRLC
                     YSGTVLHTQPSGLLRTRDPMQIGAELYGHAGLESDIEIQALMLRALQEATGVDGVHLD
                     IGHVSVFRALITRGGATAQQEADLFQVLQAKDIPALRDLSIGLDAQTRKALLLLPELY
                     GGPEVLALARQRLPAYPELTACLDALARIGRELDGDVKELCFDLAELRGYHYHSGVVF
                     AAYAGNRPEAIARGGRYDEVGRAFGRARPATGFTMDLRELAQMAKEEPEAPRILAPYR
                     PHDAALQAEITRLRAAGSVVVPDLPDHEAHRAELECSQELVQRDSAWKLMPFGTDQAG
                     RKAK"
     gene            15578..16876
                     /locus_tag="VN496_00230"
     CDS             15578..16876
                     /locus_tag="VN496_00230"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXF15395.1"
                     /translation="MASNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGRKQVLRLIPSGILRQGVNCYIGNGVVLSPTALLQEIAELESAGVEVRPRLKISD
                     ACPLILAYHVAIDQAREAAKGAEKIGTTGRGIGPAYEDKVARRAIRLQDLYIPGRFEE
                     KLREVLDFHNFVLEHYLKAKPVDFHKTHDDTMALRAELEPMIADVPRALYHAHRDGDN
                     LLFEGAQGTLLDIDHGTYPFVTSSNCVAGAAAAGAGVGPQMLHYVLGITKAYTTRVGS
                     GPFPTELDDGIGEQLRTRGNEFGSVTGRPRRCGWFDAAALKRSVQINGVSGLCITKLD
                     VLDGVEALQVGVGYKLGGERIDILPSGADASAGCEPIYEEIPGWTQSTVGITRIAELP
                     ASARHYLKRIEQACGVPIDMISTGPDREETIVLRHPFDPA"
     gene            complement(16908..16994)
                     /locus_tag="VN496_00235"
     tRNA            complement(16908..16994)
                     /locus_tag="VN496_00235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16958..16960),aa:Leu,seq:cag)
     gene            17074..19308
                     /gene="rnr"
                     /locus_tag="VN496_00240"
     CDS             17074..19308
                     /gene="rnr"
                     /locus_tag="VN496_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXF15396.1"
                     /translation="MKTPKKTSKKAAISAARKSRARAGSADIRELDPFLERERSRYES
                     PLPSREHILAVLMEQGVPVSVEKLAQLLSISEQETETFERRLAAMQRDGQIMRNRKAA
                     ICVVDKLDLIKGRVQGHPDGFGFLLRDDDGEDLFLNPAEMSKVLHGDRVVAREGGVSA
                     RGRREGHIVEVLERANRKVVGRFLKEHGIGVVAAEDRRISQDILIPAGANGGAKPGQV
                     VIAEIIDQPQRHAQPVGRIIEVLGNYADPGIEIALRKHDLPHEFSPEVEKAASKFPPG
                     VTDADLKGREDLRKLPLVTIDGETAKDFDDAVYCERHGKGFKLWVAIADVSHYVKHGD
                     PIDLEARERGNSVYFPRRVIPMLPEVLSNGLCSLMPQVDRLCMVCEMDISARGEIAAY
                     RFYPAVMHSHARLTYTQVAQAIDQPPGESARELGMLLPHLQALHALYQGLVKARALRG
                     AIDFETVETQMFFDDKGKIERIVPTQRNDAHRLIEECMLAANVSASEFLSKHEHVALY
                     RVHEGPTPEKLAALREFLREFGLQLTGGEDPHAKDYARLLEKIKARPDVQLLQTVMLR
                     SLRQAVYSPENVGHFGLAYEAYTHFTSPIRRYPDLLIHRSIKAVLAGKRYEPGNWQEL
                     GVHSSETERRADEATRDVEAWLKCYYMQDRIGERFTATVSGVANFGVFVALHEVYVEG
                     LVHISELGADYFHFDATKHQLLGERTGKRFRLGDRIPVKLVRVDLETAKIDFVLDDGA
                     RIAR"
     gene            19305..20057
                     /gene="rlmB"
                     /locus_tag="VN496_00245"
     CDS             19305..20057
                     /gene="rlmB"
                     /locus_tag="VN496_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXF15397.1"
                     /translation="MSERRVVYGLHAVTAALRRRPGAVFEVFLAGGRSDPRARRVAEL
                     AKTAGVRLHEVDVERLNGFAKGGRHQGVAAFVEQVSLAASLDDVLDDLAEPALLLVLD
                     GVQDPHNLGACLRVADGMGVHAVVAPKDRATGLTATVHKVASGAAESVPFVAVTNLAR
                     TLRELKDKGIWVVGAVGDGAVRTLFDAKLSGPLALVVGAEDEGLRRLTRETCDELLSI
                     PMLGQVESLNVSVAAGILLYEARRQRSESRKA"
     gene            20217..20963
                     /gene="rpsB"
                     /locus_tag="VN496_00250"
     CDS             20217..20963
                     /gene="rpsB"
                     /locus_tag="VN496_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXF15398.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRYWNPKMAPFIFGHRNKIHIINLEK
                     TLAMYQEAVKFVRQLSSNKGSILFVGTKRQAREIIREEAMRAGTPFVDHRWLGGMLTN
                     FKTVKISIKRLRDMELMVQDGSLEKLSKKEQLNFQREMVKLDRSLGGIKEMNGLPDAL
                     FVIDVGYQKGAITEANKLGIPVVGVVDTNHSPDGVDYVIPGNDDASRAVRLYARGIAD
                     AILEGRSQTIKEIVSDEFVEVDSSEEQATA"
     gene            21077..21961
                     /gene="tsf"
                     /locus_tag="VN496_00255"
     CDS             21077..21961
                     /gene="tsf"
                     /locus_tag="VN496_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXF15399.1"
                     /translation="MADITAGMVKELRERTGLGMMECKRALEEAGGDLKKAEDLLRIK
                     SGAKAGKMASRVAAEGAIGAYVSPDRKRGALVEINCETDFVAKDPHFAGFVKSVAELA
                     AASAITAVDALAQEQLPSGQSVESARQALVMKLGENLSVRRVAVHKAKGKLAHYLHGT
                     KIGVMLDLEGGDDVLGKDLAMHIAASKPLAISKDQVSAELIARERDIARARATESGKP
                     ANLIDKIVDGSVQKYLAEVTLLGQPFVKDDKVSVEKLLTARSAKVNAFTVFIVGEGIE
                     KKSSDFAAEVAAQAAQAR"
     gene            21978..22691
                     /gene="pyrH"
                     /locus_tag="VN496_00260"
     CDS             21978..22691
                     /gene="pyrH"
                     /locus_tag="VN496_00260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXF15400.1"
                     /translation="MKPAYKRVLLKLSGEALMGDDAYGINRETIDRITAEVKEVINLG
                     VEVGVVIGGGNIFRGVAPAAAGMDRATADYMGMLATVMNALALQDAMRRHGLKSRVLS
                     ALNIEQVAEPYIRGKAMRYLEEGRVVIFAAGTGNPFFTTDTAAALRGMEMNVDIVLKA
                     TKVDGVYTDDPKSHPDAMRYQRISFDEAIVKKLKVMDATALTLCRDQKLPICVFSIFK
                     FGALTRVVLGEDEGTLVHA"
     gene            22712..23272
                     /gene="frr"
                     /locus_tag="VN496_00265"
     CDS             22712..23272
                     /gene="frr"
                     /locus_tag="VN496_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXF15401.1"
                     /translation="MTVADVKKSADQKMHKTLEALKADLGKVRTGRAHTGILDHVHVD
                     YYGNATPLGQVANLSLLDARTISVTPWEKKMVPAIEKAIREAGLGLNPSSMGELIRVP
                     MPALTEERRRDLTKVVRAEAENARVAVRNVRRDANTGLKDLLKGKAISEDDDRRAQDD
                     IQKLTDRYIAEIDKALAAKEADLMAV"
     gene            23281..24072
                     /gene="uppS"
                     /locus_tag="VN496_00270"
     CDS             23281..24072
                     /gene="uppS"
                     /locus_tag="VN496_00270"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXF15402.1"
                     /translation="MYKSSTIAIPPVGPVPRHIAIIMDGNGRWAKSRFLPRVAGHKRG
                     LEAVRAVTKACITQGVQYLTLFAFSSENWRRPQEEVTFLMELFVIALEQEVARLHENQ
                     IRLKVVGDLSSFEPRLIRMIAEAETLTAANERLTLTIAANYGGRWDILQALNRMAQHL
                     PQPAGGYREAHLAPHLAMNYAPEPDLFVRTGGEQRISNFLLWQLAYTEFYFTEELWPD
                     FNAQSLSVAIESYRNRERRFGRTSEQLVGGVTHSDPLRESGGDSR"
     gene            24107..24934
                     /locus_tag="VN496_00275"
     CDS             24107..24934
                     /locus_tag="VN496_00275"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXF15403.1"
                     /translation="MLRQRILTAIVLLVLLLGCAFMLPSLAWQLLLCVPIALAADEWA
                     LLAGYSKRLRVAFIVVLLGSCAAIILALASEQPAGTVAAFSRLLFSVALVFWTILIPL
                     WLHRGWRVKCALSLGAAGWMVLIPAWLAAVFLQKSPFLLLAVLLVVWTADTAAYFSGR
                     RFGHRKLAPQISPGKTWEGAIGAFAAALVYGLVASLVLQPAASGYDRTATLIFVASLT
                     LLSIVGDLFESWIKRSAGAKDSGTLLPGHGGVLDRIDSLTAALPLAALHFLPALGLT"
     gene            24931..26112
                     /gene="ispC"
                     /locus_tag="VN496_00280"
     CDS             24931..26112
                     /gene="ispC"
                     /locus_tag="VN496_00280"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXF15404.1"
                     /translation="MKRLAILGSTGSIGISTLAVVDRHPDRFEVVALSADRQVDRLFE
                     QCCRYRPRFAAMASDAAAEQLRHKIVDSGLDCEVLAGVTALEQIAGAPEIDAVMAAIV
                     GSAGLPSTLAAARAGKQLLLANKEALVMAGGLLMDAVVASGAVLLPVDSEHNAIFQAL
                     PRDFDGSLSHSGVKRLWLTASGGPFLRTARELLRKVTPDQACAHPKWVMGRKISVDSA
                     TLMNKGLEVIEASFLFQAKPAQIEVVIHPQSIVHSLVEYIDGSVLAQLGNPDMRTPIA
                     HALAYPERIDAGVQSLDLFTIGSLQFERPDLDRFPCLRLAYHALEAGGNAPVVLNAAN
                     EVAVESFLSGRLPFVRIAGLVEEVIARVTASRMRTLEDVLAADAEARAVAEQELGAVG
                     I"
     gene            26131..27495
                     /gene="rseP"
                     /locus_tag="VN496_00285"
     CDS             26131..27495
                     /gene="rseP"
                     /locus_tag="VN496_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXF15405.1"
                     /translation="MSIISTLIAFVVALGSLIVFHELGHYLVARLCKVKVLRFSLGFG
                     RVLWRRKFGADQTEWAISAFPLGGYVKMLDEREGPVTREELHRAFNRQTVAKRFIIVL
                     AGPVANFLLAILLYWILFLHGVPGVRPVVGEPLPGTPAAQAQIAPGELIARVDEVPVP
                     TWQELRWVLLDRALRKATVQLELHSERGDIRFQRLDLSSIGPDKIDTDLIQAIGLTRY
                     QPPLKPVIGRLIAGGAGERDGLMEGDQVLEIDGRAIAGWEELVASVTAAPGRALQFNV
                     LRAGTEQHVVVTPESKHEGGKTLGRIGAAPKIDEAALKRLMTEVHYGALESIAKAAQR
                     TWEVSTFTLMMLGKMIIGDVSLKNLSGPITIADYAGQSAQLGWLPYLSFLALISISLG
                     VLNLMPVPLLDGGHLMYYVAEIVKGSPVSERVMEVGQQVGMVLLFSLMAFALYNDINR
                     LLGS"
     gene            27503..>28602
                     /gene="bamA"
                     /locus_tag="VN496_00290"
     CDS             27503..>28602
                     /gene="bamA"
                     /locus_tag="VN496_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXF15406.1"
                     /translation="MKKRLLFLVLSSLISLSAKAFEPFQVKDIRVEGLQRTEAGTVFS
                     YLPIKIGDTVTEERATAAVKALYATGFFKDVRLEADGNVLIVQIEERPAIAQIEINGS
                     KEFDAKQLKEGLKAVGLAESRIFDRALLERAERELKNQYISRGKYGVQIVTTITPLER
                     NRVALNFDISEGEVAKIRQINIVGNKVFKDKDLTDEFVLRTPGALTWYSKNDQYSKPK
                     LQADLENLRSFYLNQGYLEFTIDSTQVSISPDKKDIFINVNITEGKKFKVAAVKLAGD
                     LIVPEAELKALIKLKPGEVFSRERLTESTKLIQDRLGNEGYAFASINPVPEIDREKNE
                     VAFTLYVDPGRRVYVRRISISGNTNTRDEVIRR"
BASE COUNT         6234 a         7802 c         8689 g         5877 t
ORIGIN      
        1 tccttcaagg atgcattctg gggattgatg ttggtggtga ttgtcatggg cggaatttat
       61 gggggcatat ttacgcctac cgaagcggcc gctgtaagcg ccgtctacgc ctttgtcgct
      121 gccgtgttca tttatcgcgg cctttctctg aggcaggttc caaaagtcct gctttcggcg
      181 gccaatacga gcgcgatgct gctctacatt attaccaacg ccattctgtt ttcttttttc
      241 ctgacctcag agcagattcc ccaagcgctc tctcagtggg tggtagacca gcatctctcg
      301 gcatggacgt tcctgattgc ggtaaatatt gcattactgg tggccggcca gttcatggag
      361 cccagctcca taatcctgat cctggcgccc ttgctggtac cgatcgcgcg gaatctgggc
      421 gtagatgcaa ttcatcttgg cgtcatcatg acggtgaaca tggagatcgg catgatccat
      481 ccacccgttg gattgaatct gtttgttgcc agccatatcg ccaaaatggg tttgactgag
      541 gtgtccatcg cgtgcctgcc atgggtcgta gtgttgcttg catatcttgt tctgatcacg
      601 tacctaccgg gtgtctcgct gtggttaccg aacctgctgt attagacgat gttcttcagc
      661 gcggaccgac caccacggcg tcaataccca attttttggc gcgcgcgatc gcccggtcgg
      721 catcgtgctt attccggaaa ggacccattt gcacgcgtgt ttccagatgt gtcctgatgc
      781 cggcctgctg cagttgtttt tgcaaagcca gagcgttcgc aagtgaattg aatacgccgg
      841 cctgcacgat ataggtcttt gctggtgcgg cggggagcgc accagcccgc ggtgcggtcc
      901 ccgctgcgcg aggcggaacc accagggttt cgctattgag aattttcggt ggcgccggct
      961 ccggctgcgt tatcttggac atcggtgttt cagagccggg caccggcgtg gtaatgagcg
     1021 cttgggcggg ttcgtgtgga gcggttccaa taccggcggg aataccgggc aagtgcttta
     1081 ccaacgccag agcgagaatc gatactccaa tcagcgcgac cgcaatgatg agccgcctgc
     1141 cgttcggtga tttggccggt cgtcccaaaa catctggctg attcggatcg ggtggcatgt
     1201 gcgctgtcgc aaaatggatg accgatgcta acaccgattt tcgcgcgctt cgtttgccgg
     1261 atgctgcact ttgactgcaa caggaatcag ccaaaggaaa actccaggcc aaattcgttt
     1321 cattcgcttt ggcgccatgc tattcttatg aattagcttt gaaaaacaaa accttttgga
     1381 gaaattgatg gctgttgaac gaactctgtc aatcattaaa ccggatgcgg tggcaaaaaa
     1441 tgtgatcgga cagatttatt ccagatttga acagagcgga ttgaaaattg tcgcggcgaa
     1501 aatgatgcaa ttgtcccgcg gggacgctga gggattttat tcagtacatc ggcagcggcc
     1561 gtttttcaac gatctggtga acttcatgat ctccggcccg gtcatggtac aggtgctcga
     1621 aggcgaaagc gccatacaga agaatcgcga tctgatgggc gcgaccgatc caaaaaaagc
     1681 atccaaagga tcgattcgcg ccgatttcgc cgatagcatc gacgccaatg ccgtgcatgg
     1741 ttccgacgct ccgcaaactg ctgcagtgga gatcgcttat ttttttcctg cgtttaacat
     1801 ctattcacgc tgatgccggt taacctgctc gaattcgatg cgcaaggcct cgcggctttt
     1861 tttgctgaaa tcggcgagaa gccatttcgc gcgaagcagg taatgcgctg ggtccaccag
     1921 tttggacagt ccgagttcga gggcatgagt gatctgtcga aggacttgcg cgcgaagctt
     1981 aaaactggcg cgacggtgca gtcgccgtgc ctgcttgacg attccaccgc atccgatgga
     2041 acgcgcaaat ggctccttgg cgtcggcgcc gccaatggta ttgaaactgt attcatccct
     2101 gaaaccggcc gtggaacgct gtgcatttct tcgcaggtcg gctgcgcgct cgaatgcgcc
     2161 ttctgttcga ccgggaggca gggattcaat cgtaacctga cagtggctga aatcatcggg
     2221 cagctgtggt gggccaatcg cgcactcggg gcagaccgac agggagagcg gataatcagc
     2281 aatgtcgtca tgatgggcat gggagaaccg ctggccaatt tcgaaaatgt cgtatcggcc
     2341 atgaggatca tgctggacga caacggttac ggactgtcgc gtcgacgcgt cacggtgtcg
     2401 acgtcgggta tggtgccggc aatggataga ctcgccgcgc agctgccggt cgcgctcgcc
     2461 gtttcgctgc acgctcctga cgacgcacta cgcaatcgcc tggtgccgat caacaagaaa
     2521 tatccactga aggaattgat ggctgcgtgc aaacgctata tcaaggttgc accgcgggat
     2581 ttcgttacgt ttgaatatgt gatgctcgac ggtgtcaacg atagcgtgga ccaggccagg
     2641 cagctggtgg cgctggtaaa aggcgtgtcg tgcaagttca atcttattcc gttcaacccg
     2701 tttccggatt caggcttcgc acgttcttca cgagatcgta tcgcagcatt tcgcaatgta
     2761 ttaatggagg ccgggctggt cacgacgacg cggaaaaccc gcggcgaaga cattgacggc
     2821 gcttgcggcc aactggcagg ccgggtgcag gatcggacgc agcgtaaatc gcgccgaacg
     2881 atggtggccc ctgcatgaag gtggtatttt ttcttatctt aactgtcgca gttctggccg
     2941 gctgcacgac ccatccaacc aaatcagaaa ccggtgagcc gaggaacgcc gccgaaaatc
     3001 gcgctcgcat ccataccgaa ctgggcgcag cgtactacgg tatcgggcag atgccggttg
     3061 ccatccagga gctcaaggag gcaatcagtg ctgacccgaa ttatgccccg gcacatagcc
     3121 agctcggact ggtctacatg agcctgaagg aagatgcttt ggcacagcaa tccttcgagc
     3181 gcgcgctgaa gattgaccca agtgactctt ccgcgaacaa caattatgga atgttcctgt
     3241 gtcagcgcaa acgcgaaaaa gaagccatga aatatttcgc cgctgcgctt aagaatccgc
     3301 tctacggctc gccggaaaac gcctatacca acgccggcat ttgtttgcgc atgcaagggg
     3361 acgacgcaaa ggcggaggaa tggctacgca aggcactggc gctgcagccg gaccagtccc
     3421 tggcgttgta tcaattggcc gacatttcat tcaaacgcaa tgagctcatc gatgcgcgga
     3481 cactgctgaa ccgccacatg caagtatcga ctccgtcggc ggaggcctta tggcttggcg
     3541 cgcgcattga gcagcgtctc ggtaatcgca cggccctcgc cagctatggc gcgcaactga
     3601 ataatcgtta tccaggtgcg ttgcaaacca gatcattcaa tgagggaaaa tttcagtgag
     3661 tggcactggc atgatgaacg agcctgctcc gacggccgct ccttccccaa cgcccggcga
     3721 tccgggcgcg gccggcgcaa cgctgctcaa ggaacgccgg cgccagggtt tgagcctcgg
     3781 cgatatttcg cgccaactta agctttccgt gcggcaagta gaagcgctgg agcgtgacga
     3841 ttattccgca tacaaaggtc cggtttttat ccacggcttc attcgtaact acgccaagct
     3901 tcttggtctg gatccggaac cacttattcg cgctgccgac gcgctgctca agcctccaag
     3961 cgtcgcgtcg gccgactcaa caacgcagcc cgaagtgctg cgggcgcctg gaaagaccgg
     4021 caaatcgttg ccctggggcc tgatcggcct cgcgctattc gtgggcatcg caatatcggt
     4081 gtattttggc gggcgccgcg cgccggacgc tggacgcaac gcgccaaccg cggtggtgcg
     4141 cgacaatcta aattccacga agagccatcc ggaaacaaga tcgggcggcg aaaccagtca
     4201 acccaagcct cgcgttgaag tgaccaagcc aacgcccaag tcagccgcta aagctgatgc
     4261 aagggctgag ccgaaggcca ggagcgaatc tccgacgccc tcggtagaaa ctcgcgtacc
     4321 ggcagaaacg agcgtgccgg cagaaacgag cgtgccggca gaaactggtg gaccgattgc
     4381 ggtagatgaa tcggaacgcc tgacggtgag aatgattttt gaacaggatt cctgggttga
     4441 gattaaggat cgcgccggta atacaatttt cggacaactc aattccgcag gcacccgtcg
     4501 cagtgttagc ggggaacggc cgcttacggt agtagtcggc aatgccgcgg gtgtgcgtct
     4561 gtttcagggt gacaaaagca tcgaccttgc tccgtacacg agggtcgatg ttgcgcgact
     4621 aacgctggag taacatcgtt ctgccatgtc tggtaatttt gttcttcgtc accaaacgcg
     4681 cctcgtgcgt atcgccggca ttccggtagg aggtggcgcc ccggtcgtcg tgcagtcgat
     4741 gactaatacc gatactgcgg acgcaagcgc gacggcgcaa caagtccgga cgcttgcgga
     4801 ggccggatct gagttggtgc gaatcacggt aaacagcgca gaagcggcag ccgctgtccc
     4861 gcaaattcgc aatatcctgg aacgcgaccg gtgccgtgtg ccgctgattg gcgatttcca
     4921 tttcaatggc cacaagctgc tgaagcagtt tccggaatgc gctcaggttc tggacaagta
     4981 ccggataaat cctggtaatg tcggccgggg ctccaagcgg gatgagcaat tcgctacgat
     5041 gattgaaacc gcgtgccgct atggcaaacc ggtgcgaatc ggtgttaatt ggggaagcct
     5101 tgatcccgac ctgctcggcc gcatgatgga cgacaacgct cgtctgtcag agccgcaaga
     5161 agcggccgcg gtcatgaggg aagctttgat tgtttcggcc ttgcaaaacg cgctcaaggc
     5221 agaagagatc ggcctgccgg gcgatcgcat cgtattgtcc tgcaaagtga gcggcgtgca
     5281 agacttgatc gcggtctatc gcgacttggc cgcgcgttgt gattatccgt tacatctcgg
     5341 cctgactgaa gcaggcatgg gttcgaaggg catcgtagcc tccaccgcgg cgctcgctgt
     5401 ccttctccag gaaggcattg gcgacacgat tcgcatttct ctcacaccgg atccgggcgg
     5461 agaccgatcg cgtgaggtcg ttgtggctca ggaaatcctg cagaccatgg ggctgcggtc
     5521 tttcacgcca atggttattg cctgtccagg ctgcgggcgc acgacaagta cggtcttcca
     5581 ggaacttgcc gaaaagatcc aggcccatct gcgtcagcag atgccggtct ggcgcgttaa
     5641 atatccgggt gtggaagaca tgcgtgtggc tgtgatgggt tgtgtcgtga acgggcccgg
     5701 cgaaagcaaa cacgccaata ttgggatcag tttgccgggt tcgggcgaaa cgcccgttgc
     5761 accagtattc gtcgacggga taaaaacggt gacgctcaag ggtgagcata tcgccgaaga
     5821 atttcaccgg ctggtggacg cctatgttgc cagtcgtttt gggcgcaacc catccgcggc
     5881 ggcaaacagc gatgagtccg ctcgggcgct cggctgaagc gcgtccacct ctagactcca
     5941 agtttcacat ggcgcaaaca attagagctg tccgcgggat gaacgacgcc ctgccggatc
     6001 agacaccgct ttgggagttt ttcgaagaca cggtgcgtgc gtggcttgcg caatacggtt
     6061 atcgcaatat tcgcatgccg atcctggaaa aaaccgaact cttcgtgcgc tccatcggcg
     6121 aggtcaccga catcgtcgag aaggaaatgt acacctttgt ggatcagctt aatggtgaga
     6181 gtcttacgct gcgccccgag ggcactgctt cctgcgtacg cgctgtgatc gagcacaact
     6241 tgctttatag cggcgctcaa cggctgttct acatggggcc aatgtttcgc cacgagcgtc
     6301 cgcaacaagg ccgctatcgt cagtttcatc aggtgggtgt cgaggcgctt ggttttcccg
     6361 ggccggacgt cgatgtggag cacatggcca tgggagcgga tctctggcgg cgactgggac
     6421 tggaagggct ccagcttgag atcaatacgc tgggcaacac gacttcgcga ggcttgcatc
     6481 gcgcagagtt gattgcttat ctggagcgac atgcggcgtc gctcgacgac gatgcacgcc
     6541 ggcgtcttca cagtaaccca ttacgcgtgc tggacagcaa gaacccggcc ctgcaggacg
     6601 tcattgcggc tgcgcccagg cttgccgact atcttgatga cgcgtcgcgg gcatatttcg
     6661 acgcgttgca ggggctgttg aaaaacgccg gtatagattt cgttgtaaat gggcgtctgg
     6721 tgcgcgggct ggactattac aatgacgtcg tttacgaatg gacgactagc cggcttggcg
     6781 cgcagggcac ggtttgcgct ggcgggcgat acgacagcct gatcgcacaa atcggcggta
     6841 aggcggcgcc ggcatgcggt tttgcaatgg gaatcgaaag agttcttgcg ctgctcgaac
     6901 ctgcccggat tcccgcccgt gctgcggatg tctatgtcgt tcatagcggc gaagcggcca
     6961 gcccttttgc ctggaacgtc gctgctttat tgcgccggcg tgggcttgcg gtcgtgtttc
     7021 attgcggcgg cggcagcttc aagtcccaga tgaaacgtgc cgatgcaagc ggtgcgctgt
     7081 ttgcactgat cgttggagat gatgaagcgc gcaaacaaat cgtttctctc aaaccgatgc
     7141 gcaaagcggg agagcaggtt caggcggatc cggaacaggc ggcaaccctg ataatcaata
     7201 atcgttgatc aatcaccata gaaaaactcc gacatggcat tcgatcttga agagcaggaa
     7261 cagatagccg aactcaagca gttctggaaa caatacggca agcttattgt cacggttgca
     7321 gtggcggcgt tgctcgcctt cgcagccatg caaggctggc gttattacaa aaatatcgaa
     7381 tcccagcagg catccgccct gttcacgaaa tttgcggaag ctgtgcgcaa gaatgacgtg
     7441 aaagatatcc gcgagttggg ccaacaggtc atggataaat ttggctcgac ggcgtacggg
     7501 ccaactgcgg cgctgctgct cgctaaaacg aattttgaaa acggggagcc ctctgccgcc
     7561 gccggagaat tgcaatgggc gatcgaccat gccaaggatg tcgaaaccgc ggagcttgcc
     7621 cggctgaggc tcgcgagcat ccggctggac gagaagaaat acgaggaggc gctcaaactg
     7681 ctcgagacaa agcacagccc agccatggaa accttgtatg ccgacctgcg cggcgatgta
     7741 atgctcgcgc aagggaaacc cgcggaggcc cgcgccgctt ataaagccgc aattgacaag
     7801 agcttgccaa acagcacata tcgcagcgtt gtccaaatca agcttgatgc gctcggaagc
     7861 aataagtgac ccgattgaaa cttcggcttg ccatagcgtt tttgtttgtc acattgctcg
     7921 gcgcttgcgc cagcaaaggt cccgaaccag ccaggctggt tgagttcaag ccttccgcga
     7981 aactcaaggt cgcctggaaa ataaatgtgg gagaagccgg ttattacatc ttcacacctg
     8041 caatctatgg cgacagcgtt tttgccgcca gcaaccgcgg tgatttgctg cgcctcaatg
     8101 cgtcgaacgg caagacgatc tggcgtgtgg ataccaaggc tccgctctcc ggcggcgtgg
     8161 gaatcggaga gaacatggtg ttggttggca ccgcaaaggg atccgttctc gcctatgatt
     8221 tgcagggcaa accactgtgg caggcgagga tctccagcga ggtgctcagt gccccgcagg
     8281 ccgctgggga atttgtcatc gtgcgcagtg gcgacagtcg tattttcggt ctggacgcgc
     8341 gcaacggcgc acgccgatgg gagtaccaga cgacgccgcc gcccctgacg ttgcgcgcca
     8401 atccaggggt gatggtgatt gaaaatttcg tcattgcggg catgccggcc gggaagttga
     8461 tcgtgctgaa catcgccaac ggcggccttg tatgggaaac cgtcgtggcg gcgccgaagg
     8521 gagacaacga gcttgagcgc atcacggata ttgccggacc gccgctggtg gaggcgggcc
     8581 gggtttgcgc ggctacattc cagggcaggg ccgcgtgcta cgaaacgcag aagggcggcc
     8641 agttatgggc gcgtcctgcc tcgagcatag gcagtctcgc cacggatgat ctgagcgtgt
     8701 atctgtcaga ggacaccggc tccgtggttg cgcttgacag gaatagcggg gcaagcgttt
     8761 ggaaacagga caagctcgcc taccgcaatg tcagcgcgcc gcttgcgaca aaggactata
     8821 tcgtcgtcgg cgatttcgag ggtcaggtgc atttcctgaa attcgaagat gggtcattcg
     8881 ccgcgcgtct gccaactgac ggtggcggca tagcgaccgc gcccaaattg cttggcgaca
     8941 aggtgctggt gcaaacacgc aacggcgggc ttttcgcaat tgcctttgca aaatagccgc
     9001 aaccgctcgt caggctgaca cgcacgcctc gcgctttccg gaatgccaaa gtgaatccaa
     9061 ccattgtgct ggtcggccgg cctaatgtcg gcaagtcgac gctcttcaac cgaatgacgc
     9121 gttcgcgcca ggcactggtg gccgatattc caggccttac ccgggatcgc cattacggcc
     9181 atggcgaagc cggaggcaaa tcgtatctgg tcgtggacac cggcggcttc gaaccggtag
     9241 caaaggatgg aatttacaag gaaatggcac gccagacacg tcaggcggta gacgaagcag
     9301 acgcggtact gctgctcgtg gacgctcgcc agggcctgac gcccctggac cggcagatag
     9361 ccgcggaact acgcaagact gcgcgcaagt tattgctggt ggtgaacaag gcggaaggtc
     9421 tggcacacga aacggccgcc gctgaatttc acgagctggg cctcggtgag ccactgacca
     9481 tatcggcatc gcacggcgaa ggcgtgcgtg acctggtgga tttcgttctc gaggaattca
     9541 atccagacga tgccgctaca ccgccgcagg cgcagcatcc gagaatagcg gtcgtcgggc
     9601 ggccgaacgt cggcaaatcc acgctggtca atacggtgct cggcgaagaa cgggtgatcg
     9661 cgtacgataa acccggaacc acgcgcgaca gcatatccat cgacttcgag cgtaatgggc
     9721 ggttttatac gctcatcgat accgctggcg tgcgccggcg aggcagcgtg gaagacgtcg
     9781 ccgaaaaata ttccgttgtg aaaacaatgc aggcgatcga agattgcaat gtcgtcatgc
     9841 tggtgctgga tgtcacacag gagatttccg atcaggatgc acatctggcc ggattcattc
     9901 ttgaagccgg ccgcgcgctt gtcgttgcaa taaataaatg ggacgcggca aatgcagagg
     9961 cacgcgagaa ggccaagcgg gactatggcc gcaaactgaa tttcctcgat tttgcagcgg
    10021 tgcattacat ttccgcgcgc accggtaccg gcgttagcgc gctgctgaac tccgccaatg
    10081 ccgcctatgc ggccgccatg tcaaagttgc caacgccgaa gctcacaagg gtattgctcg
    10141 ctgcagtgca aaaacagtcg ccaccgcgtt ccggcgtttc tcggccaaaa cttcgctatg
    10201 cccaccaggg cggcatgaat cctccactaa taatgattca tggcaatggg ttgagtcagg
    10261 tcccggacag ctaccgccgt tacctggaac gcacgttcat ggctgctttt gaactccgcg
    10321 gcactcccgt caaggtactg ttcaagcacc agacccgtaa cccctttgca ggtcgcagca
    10381 ggaactgatt ccgccgtgtt tccgctggat ggtattgaat ctgaaaatcc agtacagtaa
    10441 acatgggtca aaaaataaaa taaggagcag ccatgagcgc aaaaggccag ttgttgcaag
    10501 acccattttt gaatgcccta cgcaaagagc acgttcccgt ttcgatttat ctggtgaatg
    10561 gcatcaagct gcaaggacag atcgagtcct ttgaccaata cgtggtcctg ctgaagaata
    10621 cggtcacgca gatggtatac aagcatgcta tttccaccgt cgtgccggcc cgtccagtga
    10681 acttgtccct ggagcatggg tcggaagact gagtgttcgt cgatccgtct ttgtatcctc
    10741 tgttgattgc accgcgcgcg ttctcccgaa tcaggggaga tgacgcttga attcgcgcgt
    10801 agcgcgcgcc ttattccccg atagcggatc cacctccgat cgtccgtggg gaagcgaccg
    10861 cgccgtccta gtcagtcttg acttcggcaa gctggatttc accgagtcgc aagaggaaat
    10921 tcgccggctt gcagaaagtg caggagtgca gccggttggc gccatcaagg gcaaacgcca
    10981 gcggccggac gcggcgactt tcgccggctc cggaaaggtg gaagagattg cgaccgccgt
    11041 gcagtcgaac gcggcatcgc ttgtactatt caaccatgat ctttcccccg cacagcagcg
    11101 taacctcgag cggcggttcg aatgccgcgt gatcgaccga agcagtctga ttctggatat
    11161 tttcgcgcaa cgtgcccgtt cccacgaagg caagcttcaa gttgagcttg ctcaacttga
    11221 gcacctgtcc acgaggctgg tgcgaggttg gactcacctt gaacgccaga aaggcggtat
    11281 cgggctgcgc ggacctggcg aaacgcagct tgaaacagac cgtcagctgt tgcgcaagcg
    11341 ggtcaaggtt ttgaaggaca agctcgatac ggtgcatcgg caacgccaaa caaggcgccg
    11401 cgccagacag cgcggtcagg cgttgtctgt ttcgctggtg ggttatacca atgccggcaa
    11461 gtcgacatta ttcaaccggc tgacccatgg tgatgcgtat gcggcggacc agttgtttgc
    11521 aacattggac acgacttccc ggcgcatcag cggtgcccac gggtccgcca tcatcatgtc
    11581 cgacaccgtc gggttcatcc gggatcttcc ccatacgctg gtagccgcgt tcagtgcgac
    11641 actggaagaa acagtgcagg ccgatttgct tctgcatgtc gtggatgctg ccagtccggc
    11701 acgcgacgat cagatctcag aagtgaacaa ggtgcttgcc gagatcaacg ccgattgcgt
    11761 tccacaggtg gtcgtgttga acaagatcga cgcagccggt ctgcaacccg gtgtcgagcg
    11821 agatcagtat ggtaagatca gttcgattcg cgttagcgcg acaacgggcg ccggaatcgg
    11881 ctatatacgc gccatgcttt ccgaattgca tgaaatgcag cgcaatgccg atggcattgt
    11941 cgcagcctga acacgaaggc tcctgtcgga gccggacttc cttcaaaatt gaaagcagca
    12001 agaaatggca ttgaatgatc cgcagtgggg aaagcgcggc aacgacggtc cgcctgatct
    12061 cgacgaatta tggcggcgcg taaaccagcg tctgaattcc ctgtttggcg gcaagggcgg
    12121 gggcggaaat accccgtctg cgccgggcgg ccggcagttt ggcagtggtt tcggcctgct
    12181 ggtcatactg gtcgtgatcg tatggctggc cagcgggttt tacatcgtcg atgccagcca
    12241 acgtggcgtg gtgctgcgct ttggaaaatt cgtggaatcg acgcttccgg gcccgcgctg
    12301 gcattttccg tggcccatgg agtcagcgga agtcgtcaac gttaccggcg tccggaccgt
    12361 ggaaatcggc taccgcaata atgtcaaaag caaggtgcag aatgaatcgc tgatgatcac
    12421 ggacgacgaa aatatcatcg acatccagtt tgccgtgcaa tacatcctca aggacccgca
    12481 ggattacctg ttcaataatc gcgacgccga caaatcggta atgcaggcgg ctgaatcggc
    12541 gatccgggaa gtcgtcggta agagcaagat ggacttcctg ctctcgggcg gcaaggaaaa
    12601 aatcgtcgaa acggtgcaga tccttctgca gaagatcctg gaccgctata aaaccggcat
    12661 cagcgtcatg cgagtcacca tgcaggatgc acaagcgccc gaggaggtcc aggctgcgtt
    12721 tgccgatgcg gtgaaagccg gccaggatcg cgagcgccag atcaatgaag gccaatccta
    12781 tttcaacgac gtcgtgccaa aggcaaaggg cacatccgca cgcctgctcc aggaaggaga
    12841 aggttatcgc cagagcgtaa tcgcaaacgc cgagggcgag gcctcgcgat tcaaccagat
    12901 tgtcaccgaa tacagcaagg caccggtcgt gacgcggcaa cggctttacc tcgatgccat
    12961 gcagcaggtc ctgtccaatt ccagcaaggt ggtggtcgac cagaaggccg gcaataacct
    13021 gctgtacctt ccgctggaca agatcatgca actcagcgcg accagcggcg cgtccgccga
    13081 tgcggcaaac aagtcatcgc ccgaatccgt gtctccgaca ccggcacccg cggaagtttc
    13141 gccgagatcc cgggatgctt tccgcaaccg ggaaagggag ccgcgatgaa attcaatatc
    13201 agtaccgtgc tgatcgcgat tgtcatagtg ctggtcattg ccagcttgag cctgtttact
    13261 gtcgaccagc gtcagaatgc catcgtattc cggctgggtg aaattgtcgc cgttaaaacg
    13321 gtgccgggat tgttcttcaa ggttcctcta gtcgataacg ttcgctattt cgactcgcgc
    13381 atccttacgc tggatgcaga agaggcgcag cgcttcgtca ctgcggaaaa caaacccgtg
    13441 ctcgtggatt cgtttgtcaa atggcgcatc atcgacgtca agcaatacta cgtcagcgtg
    13501 cagggggacg agaatctggc gcgcgtgcgg ttgacgcaag cggtcaacgg cagccttcga
    13561 gaggaattcg gcaagcgtac catccacgag gtcgtttccg gggaacgcga gaaaattatg
    13621 gatctgatgc gagaaaaagc ggatcagaca tcccgtgaaa tcggcgtcca ggtactggac
    13681 gtacgcctga agcgggttga tttcacgccc gagatcagcg aatcggtgta tggcaggatg
    13741 caggcagagc gcaagcgcgt ggccaatgaa ctacgctcaa ccggatttgc cgaggcggaa
    13801 aaaatacgtg ccgatgccga caaacaacgc caggtggtca ttgcacaggc ctatcgcgat
    13861 gcgcagcggc tgaaaggcga cggggatgcg aaggcatccg cgatttatgc acgagccttc
    13921 gaacgcaatc ccgagttcta tgctttctac cgcagtctcg aggcgtaccg gcaaagcttc
    13981 aagaataaat ccgatctcat gatccttgag cccaattcag agtttttcaa gtatatgaag
    14041 aatccggccg gccacgccaa atagcagagt acagaacggc tcaattgcgc gaatgcgcgg
    14101 cggcaaagca acgaaggagc ctatacgaat acgctgatga ccgcgatggc gctgatgctg
    14161 gtcatcgaag gcctgcttcc atttttggtt ccgaaggtct ggcgcgatgc ctttcgacga
    14221 ttgacggaac tgtcggacgg gcaagtccgt ttcatcggac tatcatcgat gctggccgga
    14281 ctgctgctgc ttttcctgac gaaatagcag aacattgcat tgttgaaccc aatcgccgtg
    14341 cgtgcagtgg actggcgcgc agaatgatca tgcaaaaatg gctgcttcct gaatacatcg
    14401 aggatctctt gccgcgcgag gccgctcgcg tggaaagcct gcgccgccgc attctcgatc
    14461 tttttttcgt gcatggctac gaactggtcg tgccgccact gctggaatac ctggaatcgt
    14521 tgctgaccgg gactggccac gatctggatt tgagaacatt caaacttgtc gatcagttat
    14581 cgggaaggtt actggggttg cgcgccgaca tgacaccaca ggtcgctcgc atcgatgcac
    14641 acttgctgaa tcgtaaaggc gttacccgat tgtgttactc cggcacggtg ttgcatacac
    14701 aaccttcggg actattgcgt acgcgagatc ccatgcagat cggcgccgag ctctatggac
    14761 acgccggcct ggaaagcgat atcgagattc aggcgctgat gctgcgcgcg ctacaagagg
    14821 cgacgggcgt tgacggcgta caccttgaca tcggacacgt gtcggtattt cgtgcgctga
    14881 tcacgcgcgg tggcgcaacc gcccagcagg aagcggattt gttccaggtg ctgcaggcta
    14941 aggacatccc cgcgctacgc gacctgtcga ttgggctgga cgcgcaaacc cgcaaagcat
    15001 tgctgttgtt gccggaactc tatggcggtc ctgaagtgct ggctcttgcg cgccagcgcc
    15061 tgccagccta tccggaactg acagcgtgcc tcgacgcact tgcacgaatc gggcgggagc
    15121 ttgacggtga cgtgaaggag ctctgcttcg atctggcgga actgcgtggt tatcattacc
    15181 acagcggcgt cgttttcgcg gcttacgcgg gcaaccggcc tgaagcaatt gcgcgaggcg
    15241 gtcgctatga cgaagtcggg cgcgccttcg gccgtgcccg tccggcgacg ggtttcacga
    15301 tggatctacg cgaactggct cagatggcaa aggaagaacc agaggcgccg cgcatactcg
    15361 cgccataccg gccgcatgat gccgcgcttc aggccgaaat tacacgattg cgggctgctg
    15421 gttccgtggt cgtgccggat ctgcccgatc acgaagcaca ccgcgcggaa ctcgaatgtt
    15481 cgcaggaact ggtccagaga gatagcgcct ggaagctgat gccgttcggc acggatcaag
    15541 ccggccgcaa agcaaaataa ttcataaaag gatagtgatg gcgagcaacg tcgtagtcat
    15601 cggcacgcaa tggggtgacg agggcaaagg aaagattgtg gattggctga cggatcacgc
    15661 acagggcgtg gttcggttcc agggcgggca taacgctggt catacgctgg tcataggcgg
    15721 acgcaagcag gtgctccggc tgatcccgtc cggcatcctg cgccagggtg tcaattgcta
    15781 tatcggcaac ggtgtcgtct tgtcgcccac cgctctgctg caggaaatag ctgaactcga
    15841 atccgccggg gtcgaggttc gtccccgcct gaagatcagc gacgcgtgtc cgttaatact
    15901 cgcgtatcac gtggcaatcg atcaggcccg cgaggctgcg aaaggcgccg aaaaaatcgg
    15961 cacgaccggc cgtggcatag gaccggctta cgaggacaag gtagcgcggc gcgcgatccg
    16021 cctccaggat ctttacatcc cgggcaggtt cgaggaaaaa ctccgtgaag tgctggattt
    16081 tcacaatttc gtgctggagc attatcttaa agcgaagcca gtggacttcc acaagacgca
    16141 tgacgacacc atggcgttgc gagccgaact tgagccgatg atcgcggatg tgccgcgcgc
    16201 gctttatcat gcgcaccgtg atggcgacaa cctgctattc gaaggcgccc agggaacgtt
    16261 gctggatatc gaccatggca cttatccgtt cgtaacttcg agcaattgcg ttgcgggagc
    16321 ggccgcggcg ggcgccggtg tcgggccaca gatgctgcat tatgttctgg gaatcaccaa
    16381 agcctataca accagagttg gttcgggacc gtttccgacg gagctggacg acggaatcgg
    16441 cgaacaattg cgcacgcgag gcaacgagtt cgggtcagtc accggccggc cgcgacgttg
    16501 cggctggttt gatgccgcag cgctcaaacg ctcggtgcag atcaacggcg tgtcaggact
    16561 ctgcattacc aagctcgatg tgctcgacgg cgttgaagcg ttgcaggtgg gcgtaggata
    16621 caaactgggc ggcgagcgca tcgatattct accgtctggc gccgatgctt cagccggctg
    16681 cgagcccatc tacgaggaaa ttcccggatg gacgcagagc accgtcggga ttacgcgtat
    16741 tgccgaattg ccagccagcg cgcgccatta tctcaagcga atcgagcagg cttgcggcgt
    16801 gccaatcgac atgatctcca ccgggcccga ccgcgaagaa accattgtgc tacgccaccc
    16861 attcgatccg gcgtagcagc ggcacaggaa gcattgcagg gagtgattgg tgccgagaag
    16921 aggactcgaa cctccacagt gttgccaccg ccaggacctg aacctggtgc gtctaccaat
    16981 tccgccatct cggcacggaa gggcgcaaaa tgtaatggga aagtgctgct ttgtcaacga
    17041 aacggccgct cgtttcagaa tcaaattgcc tgaatgaaaa ctccaaaaaa aacatctaag
    17101 aaagctgcga tttccgccgc aagaaaaagc cgggcgcggg ccgggagcgc cgatatccgc
    17161 gagcttgatc cattcctgga gcgcgaacgc tcgcgctacg aaagtccact cccgagtcgt
    17221 gaacatattt tggccgtgct gatggagcaa ggcgtcccgg tttccgtgga aaaactggct
    17281 caattgctgt ccatttcaga gcaggaaacc gagacattcg aacgtcgcct ggccgccatg
    17341 caacgtgacg gccagatcat gcgcaatcgc aaagctgcca tttgcgtcgt ggacaagctt
    17401 gatctcatca aaggcagggt ccaaggacat ccggatgggt tcgggttcct gctgcgagac
    17461 gacgatggag aagacctgtt cctgaatccg gccgaaatgt ccaaggtgtt gcatggggat
    17521 cgcgtggtcg cgcgcgaagg cggtgtcagc gcgcgcggac gacgtgaggg gcatatcgtc
    17581 gaagttctcg agcgggcaaa tcgaaaagtg gtcggccgtt tcctgaagga gcacggaatt
    17641 ggcgtggtgg ctgcggagga ccggcgcatc agtcaggaca tccttattcc ggcgggtgcg
    17701 aacggcggtg ccaagccggg tcaagtcgtg atcgccgaga taatcgatca gccgcaacgc
    17761 cacgcccaac cggtaggccg catcatcgag gtactgggca actatgccga tccgggaatc
    17821 gaaatcgcat tgaggaagca cgatctgcct cacgaatttt cgccggaagt tgaaaaagcg
    17881 gcctccaaat ttccgcctgg cgtgactgac gcagatctca agggacggga agatttgcgc
    17941 aagctgcctc ttgtgaccat cgatggagaa acggcaaagg atttcgatga tgcggtttat
    18001 tgcgagcgcc acggcaaggg attcaagctc tgggtggcga tagccgacgt cagccattat
    18061 gtgaagcatg gggatccgat cgatctggaa gcgcgcgagc gcggcaactc ggtatatttc
    18121 ccgcggcgcg tgattccgat gctccccgaa gtgctgtcca atggcctgtg ttcactgatg
    18181 ccacaagtgg accggttgtg catggtgtgc gaaatggata tctccgcgcg gggagagatc
    18241 gcggcatatc gcttctaccc cgcagtcatg cattcgcacg cgcggctgac gtatacgcag
    18301 gttgctcagg caatcgacca gccgccgggg gaatccgcac gcgaactcgg aatgctgctt
    18361 ccccatttgc aggcactgca tgcgctctat cagggcctgg tgaaagcacg cgcattgcgt
    18421 ggcgcaatcg atttcgagac cgtggaaacg cagatgtttt tcgacgacaa gggaaagatc
    18481 gagcgcattg tcccgacaca acgcaatgat gcccatcgtc tgatcgaaga atgcatgctc
    18541 gccgcgaacg tcagcgcatc ggagtttctg tcgaaacacg agcatgttgc actgtatcgc
    18601 gtacacgaag ggcccacgcc ggagaagctt gcggcgctgc gcgagtttct gcgcgagttc
    18661 ggccttcagt tgaccggcgg cgaggatccc cacgccaagg actatgctcg cctgctggaa
    18721 aagatcaagg ctagacctga cgtgcaattg ctgcagaccg tgatgctgcg ctccctgcga
    18781 caagcagttt acagcccgga aaatgtgggc catttcggac ttgcatacga ggcgtatacg
    18841 catttcacct cgccgatccg gcgctatccg gatctgctca ttcaccggtc catcaaagcg
    18901 gtactggccg gcaaacgcta tgaaccgggc aactggcagg agcttggcgt ccattcatcc
    18961 gaaacggaac gccgcgcgga tgaagccacg cgcgatgtcg aggcctggct caagtgctac
    19021 tacatgcagg accgcatcgg agagcgattt acggcaacgg tgagcggcgt ggcaaatttt
    19081 ggcgtgttcg tcgcgctgca cgaggtttat gtggaaggac tggtacatat ctcggagctt
    19141 ggagccgact atttccattt cgatgcgacg aaacaccagc tgcttggaga gcgcacggga
    19201 aaacgctttc gcctcggcga tcgtataccg gtgaaactgg tgcgcgtcga tcttgaaaca
    19261 gcgaagattg atttcgttct ggacgatggc gcgagaatcg cgcgatgagc gaaaggcggg
    19321 tcgtgtacgg attgcatgcc gtgacggccg cgctaagacg ccgcccgggt gcagttttcg
    19381 aggtgttcct ggctggcggt cgcagcgacc cgcgggcgcg ccgtgttgcc gaactggcca
    19441 agaccgctgg ggtacgattg cacgaggtcg acgtagaacg cctgaatgga tttgccaaag
    19501 gcgggcgtca ccagggcgtg gcggctttcg tggaacaggt ctccctagcg gcctcgctcg
    19561 atgatgtgct ggacgatctt gccgagcctg cgctgttact ggtactcgat ggcgtgcaag
    19621 atccgcacaa cctgggcgcc tgcctgcggg ttgcggacgg catgggagta cacgcggttg
    19681 tcgcgcccaa ggatcgcgcg accggcttga ccgccacggt acataaggtc gcctcggggg
    19741 ccgccgagag tgtgccgttt gtcgccgtaa cgaacctggc cagaacactg agagaactga
    19801 aagataaggg catttgggta gtgggcgcgg tgggggacgg cgccgtcagg acgctattcg
    19861 acgcgaagct ttcggggcct cttgccctgg tggtcggtgc cgaggacgag gggttgcgca
    19921 ggctcacgcg cgaaacctgc gatgaactcc tgtcgattcc aatgctcggg caggttgaga
    19981 gcctgaacgt ctcggtcgcg gcgggcattt tgctctatga ggcccgccgt cagcggtccg
    20041 aatcccgtaa ggcctgatca ggcttaaaga aggttgaggc gccgacggcc tcgctgctat
    20101 actttcaagt taaccgcgga cacgtttagc tcgcggcaaa tccacacgcc agccaagtaa
    20161 tgggtgcccg tcacagggtc cggccggcgg aggcttaacc cttacaggag aacagaatgt
    20221 cggtcaccat gcggcaaatg ttggaagcgg gcgtccattt cggacatcaa acccgttact
    20281 ggaatccaaa gatggcgcct ttcattttcg gccatcgcaa caagattcat atcatcaatc
    20341 tcgaaaagac cctggcgatg taccaggaag ctgtcaaatt cgtgcgtcaa ctctcttcga
    20401 acaaaggcag catcctgttc gttgggacca aacgccaggc gcgtgaaatc attcgcgaag
    20461 aggccatgcg agccggcacg cctttcgtcg atcatcgctg gctgggtggg atgctgacca
    20521 atttcaagac cgtaaaaatt tcgatcaagc gcttgcgtga tatggaattg atggtgcagg
    20581 acggctccct ggagaagctt tctaaaaagg aacagctcaa tttccagcgt gaaatggtga
    20641 aacttgatcg cagcctgggc ggcatcaaag aaatgaacgg gctgcccgat gccttgttcg
    20701 tcatcgatgt tggctatcag aagggcgcca tcaccgaagc gaacaagctc ggcattccgg
    20761 tagtgggtgt ggtggatacc aatcattcgc cagatggtgt cgactacgtc attccaggca
    20821 acgacgacgc cagtcgcgcg gtgcgcttgt atgcgcgcgg aattgcggac gcgatcctcg
    20881 agggacgcag ccagaccatc aaggaaatcg tgagcgacga attcgtggaa gtcgacagtt
    20941 cggaagaaca ggcgaccgcg tgattgattc tcgcgtcgca aacaaagggg cgaacgcccc
    21001 tttttttgca actggacttt ataactgaga cgctggcgga acaacgagcg gacggtactc
    21061 aaggagcaag caagatatgg cggacattac cgcgggcatg gtcaaggaac tgcgtgagcg
    21121 caccgggctc ggcatgatgg aatgcaagag ggcgctcgaa gaagcgggcg gcgatttgaa
    21181 aaaggcggag gatctgttgc gcatcaagag cggtgccaag gcgggtaaga tggcgagccg
    21241 tgtagcggcc gagggcgcca tcggcgccta tgtttcgcca gaccggaagc ggggcgcgtt
    21301 ggtcgaaata aattgcgaaa ctgattttgt cgccaaggat ccgcatttcg ccgggttcgt
    21361 aaaatcggtt gctgaactgg cagccgccag tgctatcact gcggtcgatg cgctggcgca
    21421 agagcaactc ccctcaggac agagcgtcga atcggcacgc caggcgcttg tcatgaagct
    21481 gggcgagaac ctgagtgtgc gccgcgttgc ggtccacaag gcgaagggca agcttgcgca
    21541 ttatctgcat ggcacgaaga tcggcgtaat gctcgacctg gaaggcggcg atgatgttct
    21601 tggcaaggat ctggcgatgc atattgcggc atccaagccg ctggccattt caaaagatca
    21661 ggtgtcggct gagctgatcg cgcgtgaacg ggatattgcg agggcgcgcg cgacggagtc
    21721 tggcaagccg gccaatctca ttgacaagat cgtggatggc agcgtccaga agtatcttgc
    21781 ggaagttact ttgctcggac agccctttgt gaaggatgac aaagttagcg tcgaaaagct
    21841 gctaacggcc aggagcgcaa aagtgaatgc cttcaccgtg ttcatcgtcg gcgaaggtat
    21901 cgaaaaaaaa tcgagcgatt ttgccgcgga agtcgctgcc caggccgctc aggcgcgttg
    21961 aacgcaccga gccgcgcatg aaaccagcgt acaagcgtgt cctgctgaaa ctatcgggag
    22021 aagccctgat gggggatgac gcttatggta tcaatcgcga aaccatcgat cgcatcactg
    22081 ccgaggtgaa ggaggttatt aacctcggcg tcgaagtagg cgtagtaatc ggcggtggca
    22141 atatctttcg cggcgtggca ccggccgcgg ccggcatgga ccgggccacg gccgactaca
    22201 tgggtatgct tgccacggtg atgaacgcgc tggccctgca ggatgcgatg cgccgccatg
    22261 gactaaagag ccgcgtacta tcggccctga atatcgagca ggttgccgaa ccatatatcc
    22321 gcggcaaggc aatgcggtat cttgaagaag gcagagtcgt catttttgcc gccgggaccg
    22381 gcaatccttt tttcaccacc gatacggcag cggcattgcg cggcatggaa atgaatgtcg
    22441 acattgtgtt gaaggcgacc aaggtggatg gcgtctatac cgatgacccg aagtcgcatc
    22501 cggatgccat gcgctaccag cgcatcagtt tcgatgaggc tatcgtcaag aagctcaagg
    22561 tgatggatgc taccgccctg acactgtgcc gcgatcagaa gctgccgatt tgcgtgttca
    22621 gcatattcaa atttggcgcg ctgaccaggg tagtcttggg cgaggacgaa ggtacgctgg
    22681 tccacgcctg aagtcgtgag gagaaaagga catgaccgtc gcagacgtca agaagtcagc
    22741 cgaccagaaa atgcacaaga cgctggaagc gctaaaggcc gacctgggca aggttcgcac
    22801 cggtcgggcg cacaccggaa ttctcgacca tgtccacgtg gactactacg gcaatgcgac
    22861 accactcggc caggtggcaa acctgtcgct cctcgacgcg cgcacgatta gcgtgacccc
    22921 ttgggaaaaa aagatggtcc cggcgatcga aaaggctatc cgggaggctg gtcttggact
    22981 caatccgtca tcgatgggcg agctgatcag ggtgccgatg cccgcgctca ctgaggaacg
    23041 acggcgtgat ttgaccaaag tcgtgcgggc ggaagcggaa aatgcgcggg ttgccgtgcg
    23101 caacgtgcgg cgtgatgcaa acacggggct aaaggattta ctcaagggca aagcgatttc
    23161 agaagacgac gaccggcgcg cgcaggacga catccagaag cttactgatc gctacattgc
    23221 cgaaattgac aaggctcttg cggcaaagga agcggattta atggccgtct gataacccgc
    23281 gtgtacaaga gctcgaccat cgcgatacct ccagttggcc cagtgccgcg ccatatcgcc
    23341 attatcatgg acggcaatgg acgctgggca aaaagccggt tcctgccgcg agttgccggc
    23401 cacaagcgcg ggcttgaggc cgtgcgcgcc gtgacaaaag catgcataac acagggtgtt
    23461 caatatttga ccttgttcgc ctttagcagc gagaactggc gccgtccgca ggaggaagtg
    23521 acgtttctga tggaattgtt cgtgattgcg cttgagcagg aagtcgcaag actgcacgaa
    23581 aatcaaatcc ggttgaaagt cgtgggcgat ctttccagct tcgagccacg cctgatcagg
    23641 atgattgcgg aagccgagac gctgaccgca gcaaacgaga ggcttacctt gacgatcgct
    23701 gcaaattacg gcggccggtg ggatatcttg caggcgctaa accgcatggc gcaacatctc
    23761 ccgcaaccag cgggaggcta ccgggaggcg catctggccc cgcatctggc gatgaattac
    23821 gctcccgaac cggacctgtt cgttcgtacc ggcggtgaac agcgcatcag caatttcctg
    23881 ctttggcagc ttgcgtatac cgaattttat tttaccgaag aactttggcc cgatttcaat
    23941 gctcaatcgt tgagcgtcgc cattgagtcc taccgaaacc gcgagcgcag attcggtcgc
    24001 accagcgagc agctcgtcgg cggcgttacg cattccgatc cgctgcgtga gtccggtggg
    24061 gattcccgct aaccttacag cggcgagttc gtccgccgcc gcatcgatgc tcagacaacg
    24121 catactgacc gccattgtgc tgttggtcct tttgctcgga tgcgcgttca tgctgccgag
    24181 cctggcctgg cagttgctgc tgtgtgttcc aattgcgctt gccgcagacg aatgggcact
    24241 tctcgccgga tacagcaagc gcctgcgcgt cgcgttcatc gtggtgttat tgggaagttg
    24301 cgctgcgatc atcctcgcgt tggcgagcga gcaaccggct ggcacagttg ccgcattttc
    24361 ccgcctgttg ttttcagttg cgcttgtgtt ctggaccatc ctgattccgc tgtggttgca
    24421 ccgaggttgg cgcgtgaaat gcgcgctgtc gctgggggct gccggctgga tggtgctgat
    24481 cccagcgtgg cttgcggccg tctttttgca gaagtctccg ttcttgttgc tcgcggtact
    24541 gcttgtcgtg tggactgccg atacggctgc gtatttttcg gggcgccgct tcgggcatcg
    24601 caaactggct ccgcaaatca gtccgggtaa aacctgggaa ggtgccatcg gtgctttcgc
    24661 agccgccctg gtctatgggt tggttgcgag cctggtgctc cagccggccg ccagcggcta
    24721 cgatcggaca gccaccctga tttttgtcgc aagtttgacg ctgctcagca ttgttggaga
    24781 cttgtttgag tcgtggatca agcgcagcgc tggcgcgaaa gacagcggca ccttgctgcc
    24841 tggacatggc ggtgtgctcg atcgcatcga cagtctcacg gcggcattgc ccctggctgc
    24901 gctgcatttt ctacctgcgc ttggcctcac atgaaacggc ttgccattct tgggtcgacg
    24961 ggcagcatcg gcattagcac gctggcggtg gtggatcgcc accccgatcg attcgaagtg
    25021 gtagcgcttt cagccgaccg gcaggtagac cgcttgttcg agcagtgctg ccgctatcgc
    25081 ccccgcttcg ctgcgatggc gagcgacgca gcagccgagc aactgcgtca caagattgtc
    25141 gattctggcc tggattgcga ggtattggct ggcgtgacag ctctcgagca gattgcaggc
    25201 gctcccgaaa tcgatgcggt aatggcggca attgttggtt ctgcgggatt gccttccacg
    25261 ctggcagctg cccgcgccgg caagcagctg ctgcttgcca acaaggaagc actggtcatg
    25321 gcgggaggct tgctgatgga tgcggtagtg gccagcggtg ccgtgctgct tccggtcgac
    25381 agcgagcaca atgcaatttt tcaggcgttg ccgcgcgact tcgatggcag cctttcgcat
    25441 tcgggggtga aacgcttgtg gctcacggcg tctggcgggc cgtttctgcg cacggcgcgc
    25501 gaactgctcc gcaaggttac gccagaccaa gcctgcgctc atccaaaatg ggtgatggga
    25561 cgcaagattt cagtcgattc cgcgacactg atgaacaaag gcctggaggt aatcgaggcc
    25621 agttttctgt tccaggcgaa accggcacag atcgaggtgg tcatacaccc gcaaagcatt
    25681 gttcactcgt tggtcgaata catcgatggc tcggtgctcg cgcagttggg aaacccggat
    25741 atgcgcacgc cgatcgcaca tgccctggcg tatccggagc gcatcgatgc gggcgtgcaa
    25801 tcactggatc tgttcaccat tggttcactg caattcgaac gacccgacct ggatcgcttc
    25861 ccatgcttgc gccttgccta ccatgcccta gaagcgggcg ggaacgcgcc ggtggtattg
    25921 aatgcggcca acgaggtcgc cgtggagagc tttttgtccg ggcgtttgcc gtttgtccgg
    25981 attgcgggcc tggttgaaga agtgatcgcg cgtgtgacgg ctagccgcat gcgcacgctt
    26041 gaagatgtgc tggccgccga tgccgaggcg cgcgcagtgg ccgagcagga actgggcgct
    26101 gtagggatat aaccggaata tagcgagatc atgtccatta tttcgactct gattgcattc
    26161 gtcgtcgcgc ttggttcact catcgttttc cacgaactgg gccattacct ggttgcgcga
    26221 ttatgcaagg tcaaggtact gcgtttctcg ctgggattcg gacgcgtact gtggaggcgc
    26281 aaatttggag cagaccagac ggagtgggcg atatcggcgt tcccgctcgg cggctacgtc
    26341 aagatgctgg atgaacgtga aggcccggtc acgcgcgaag aattgcaccg cgcgttcaat
    26401 cggcaaacgg tggcgaagcg tttcatcatt gtgcttgcgg gaccagtagc taattttctg
    26461 ctcgccatcc tgctctactg gattttgttc cttcatggcg tgcccggtgt gcgaccagta
    26521 gtcggcgaac cgctgccggg caccccggcc gcgcaggcgc aaattgcgcc gggcgaattg
    26581 atcgcacgcg tcgatgaggt tccagtgccg acctggcaag agcttcgttg ggtgctgctg
    26641 gaccgtgcat tgcgcaaggc gactgtgcag ctggaactgc atagcgaacg tggcgacatc
    26701 cgtttccagc ggctcgatct gtcatccatc ggtccggaca aaatcgatac cgacctgata
    26761 caggcaatcg ggttaacgcg ttaccagccg ccactgaagc cggtgatcgg acggcttatt
    26821 gccggtggcg cgggcgagcg cgacggcctc atggaaggag atcaggtact tgagatcgat
    26881 ggtcgcgcta tcgcgggatg ggaagagctc gttgcgtctg ttacagcggc gcccggacgc
    26941 gcgctgcagt tcaatgtcct gcgcgcaggc actgagcagc acgtggtcgt cacacctgaa
    27001 tcaaaacacg agggtggcaa gacattgggg cgcataggcg ctgctcccaa gattgatgaa
    27061 gccgcgctca agcgcttgat gacagaagtg cactatggag cgctggaatc cattgccaaa
    27121 gcggcacagc gtacatggga agtgtcgacc tttacgttga tgatgctcgg caagatgatc
    27181 atcggggacg tttcgctcaa aaatctcagt ggtccgatta ctatcgcgga ctacgccggg
    27241 caatcggcac aacttggctg gctgccttac ttgagttttc tcgcactgat cagcatcagc
    27301 ctgggtgtgc tcaatctcat gccggtgccg ttattggacg gcggacacct aatgtattat
    27361 gttgccgaaa tcgtcaaggg cagcccggta tcggaacggg tgatggaggt ggggcagcaa
    27421 gtcggaatgg tactgctctt cagcttaatg gcgtttgcac tttataacga tataaatcgc
    27481 ctattgggca gctaacgagc ggatgaagaa gcgattattg tttctggttc tttctagcct
    27541 catcagttta tccgccaaag ctttcgaacc cttccaagtc aaggatatcc gcgtagaagg
    27601 tctgcaacgc accgaagccg gaaccgtatt ctcctacctt cccatcaaaa tcggcgacac
    27661 ggttactgaa gaacgtgcca ctgccgcagt caaggcgctt tacgccaccg gatttttcaa
    27721 ggatgtcaga ttggaggctg acggcaacgt cctgatcgtg cagatcgagg aaaggccggc
    27781 aatcgcccag atcgaaataa acggttccaa ggagtttgat gccaagcagc tcaaggaggg
    27841 gctgaaggca gttggcctgg cggagtcgcg tattttcgat cgcgcgctgc tggagcgcgc
    27901 agaacgcgaa ctaaagaacc aatacatcag tcgcggcaaa tacggagtgc agatcgtcac
    27961 caccatcacg cccctggagc gcaatcgcgt ggcgctcaat ttcgacatat ccgagggaga
    28021 ggtggcaaaa atccgccaga tcaatatcgt cggcaacaag gtattcaagg acaaagacct
    28081 taccgatgaa tttgtcctgc gcacgcccgg cgcgctgacc tggtacagta aaaacgacca
    28141 gtattccaaa ccaaagctcc aggccgatct cgaaaatctt cgttcgttct atctgaacca
    28201 gggctatctc gaattcacaa tcgattccac tcaggtgtcc atttcccctg acaagaaaga
    28261 tattttcatc aacgtcaata tcaccgaggg caagaagttc aaagttgcag ccgtcaaact
    28321 ggccggcgat ctgatcgtgc ccgaggcaga gctgaaagcg ctgatcaagc tgaagcccgg
    28381 cgaggtattc tcacgcgagc gcctgacgga gtcgaccaag ctgatccaag acaggcttgg
    28441 gaatgaaggc tatgcgtttg ccagcattaa tccggttccc gagatagatc gggagaagaa
    28501 cgaagtcgca ttcacgctat acgtcgatcc cggacgccgg gtctacgtcc gccgcatcag
    28561 catttcagga aacacgaata cgcgcgacga agtcatccgg cg
//
LOCUS       DATMZQ010000008         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1040605, whole genome shotgun sequence.
ACCESSION   DATMZQ010000008 DATMZQ010000000
VERSION     DATMZQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040605"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..500)
                     /locus_tag="VN496_00295"
     CDS             complement(99..500)
                     /locus_tag="VN496_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15407.1"
                     /translation="MPMTHFPQVHPQRRSPRIQLGGSVLAQVVMEDGGRTKAKLQTVS
                     ITGGLLRLAKSLSQGDFVEVAFQTQSGIVRGMAEMLTPVRAATEGSLQPFRFVALGDD
                     DHRTLRMVVDSAADRSFQGIRSSQWAAPKSV"
     gene            complement(657..2225)
                     /locus_tag="VN496_00300"
     CDS             complement(657..2225)
                     /locus_tag="VN496_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXF15408.1"
                     /translation="MKIVSAAEMREIDRVTSERFGAPSLTLMENAGTAVAEFVASRFP
                     AAKSIGVICGKGSNGGDGFVIARKLHEGGKNVRLLLLAQRSELRGDAAEMFTKLPLLP
                     VIAVSPDQLKTESARAVFDAEVLLDAILGTGFRPPVGVLYGEAIAVMNASSAPVVAVD
                     IPSGADADVMGEQVGAVARADAIVTFTAPRPAHIFGILTTGPTLISPIGSPDAAIISS
                     LKLNLTTAREIAPLIGPRPVASNKGNFGHVLVLGGSTGKAGAAAMAGMAALRCGAGLS
                     TVATAKSVLAIVGGFHPELMTEPIEETEAGSISSVSLEQGRMDALVIGKTVLAVGPGI
                     SRHPDTSAFVRGVVKKYKMQIVLDADGLNAFENRAAELKDKAGALVITPHPGEMARLT
                     GLTIAAVQRDRLNVARSFAREHELIVVLKGNRTLVAQPDGTVWVNTTGNPGMATGGTG
                     DILTGMVAGLVAQNPGRVAAAVIAAVHLHGLAGDVARESMGEYSLVATDLLTALPEAF
                     RRTRHAVGESRVVI"
BASE COUNT          528 a          810 c          747 g          507 t
ORIGIN      
        1 aaggggaggg cacgatttaa agtcgtgccg aatgccgccg ggaacgttcg cggcttcagc
       61 tgctgaggtt tgcgtttact tccgccgcgc cgtttctctc aaaccgattt cggtgctgcc
      121 cactgactgg acctgattcc ctgaaaactc cggtccgcag ccgaatcaac caccatgcgc
      181 aaggtgcgat ggtcgtcgtc gccaagcgcg acgaagcgga agggctgcag tgacccttcg
      241 gtagccgcgc gcaccggggt cagcatttcg gccatgcctc ttacgatgcc cgattgggtt
      301 tggaatgcga cctcgacaaa atctccctga ctgagagatt tggccaggcg caacagcccg
      361 ccggttatcg aaacggtttg aagtttggct ttggtgcgcc caccatcttc catgacgacc
      421 tgggccagaa ctgaaccacc aagctgaatg cgtggcgagc ggcgctgagg atgcacttgg
      481 gggaagtgtg tcataggcat gactatttca cgtcgctact cgccggggca ggttaccgaa
      541 ggcatatgta ccgatttcag gcgcaaaatt gtccgttcaa tgccgagctt gcgttttcag
      601 caagatagcc gtctgccgct cgccaaaagt gaggtactac gaagctgcgt gatcagtcag
      661 atcacgactc gggactcacc tacggcatgc cgtgtccggc gaaatgcctc aggcaacgcg
      721 gtaagcagat cagtcgcaac cagcgagtat tcccccatac tctcacgcgc cacatcgccc
      781 gccaggccgt gaaggtggac ggctgcaatc accgccgccg cgacgcgacc aggattctgc
      841 gcgactagac ccgccaccat gcccgtcaat atgtcgcccg tgcctcccgt cgccatgcca
      901 ggattgcccg tggtgttgac ccagactgta ccgtcaggct gcgcaaccaa agttcgattg
      961 cctttgagga caacaatcag ttcatgttca cgagcaaagc tgcgggccac attcagccgg
     1021 tcgcgctgta ccgcggcaat ggtcaaaccg gttagccggg ccatctcgcc aggatgcgga
     1081 gtgatgacga gggcgccagc cttgtctttc agctcggctg cgcggttctc gaaggcgttg
     1141 aggccgtcgg cgtccaggac aatttgcatc ttgtatttct tgaccacgcc tcgcacaaag
     1201 gccgaggtat cggggtggcg cgaaatgcca gggcccaccg ccagcaccgt ctttcctatc
     1261 accagagcgt ccattcggcc ttgttccagc gagaccgaag aaatgctgcc tgcttccgtt
     1321 tcctcgatcg gctcggtcat cagctcggga tgaaagccgc cgacgatcgc gagcactgac
     1381 ttcgcggtgg caaccgtgga caggcctgcg ccgcagcgca gtgccgccat gccggccatg
     1441 gccgccgccc ctgccttgcc ggttgacccg ccgagcacca gtacgtggcc gaaattccct
     1501 ttgttcgaag ccacgggccg tggaccgatc agcggagcga tttcgcgcgc tgtagtcaga
     1561 ttgagcttca gagacgagat gatggctgca tccggcgaac cgatgggcga aattagcgtc
     1621 ggcccggtgg tgagtattcc gaaaatgtga gccggacggg gcgcggtgaa cgtcacgatc
     1681 gcgtccgccc gcgccacggc gccaacctgc tcgcccatga cgtccgcgtc cgccccggac
     1741 ggaatatcta ctgcgactac aggcgcggag ctcgcattca tcacggcgat tgcttcgccg
     1801 tacagtacgc ctaccggcgg gcgaaagcca gtgccgagga tggcgtcaag cagaacctcg
     1861 gcgtcaaaaa ccgcgcgggc cgattcggtc ttcaactgat caggagaaac cgcaatcacc
     1921 ggcaacagcg gcagcttggt gaacatctcg gcggcatcac cgcgcagttc cgatcgctgc
     1981 gccagcaata gcaatcgcac gttttttccg ccttcgtgca gtttgcgggc aatgacaaac
     2041 ccgtctcctc cgttacttcc cttcccacaa attacgccga tgcttttggc cgcaggaaaa
     2101 cgcgatgcca cgaactctgc cactgccgtg cccgcgttct ccatgagcgt cagcgacggg
     2161 gcgccgaagc gctcgctggt gacgcggtcg atctcacgca tttcagcggc agagacgatt
     2221 ttcatggtgg ttgttgtggt cagccgttaa acatcctgtc atcccgaccg gaaaagcgca
     2281 gtgagcgtgg tggagaggaa ccttgcgttt cggcagattg cggccttacc aaggctgcgg
     2341 ttctgagatt aactgagagc tcacgatcag caacgaccgt gacaatggaa cacaaaaggt
     2401 cccgccacta cgctggctgc gctttgcccc ggtcgggatg acaacacgaa acatcacctt
     2461 cgctaaacaa gcatttgcca tgagcatcgg ccaacgaccg tcgaccaaca accatcgatc
     2521 aacaaccatc gatcaacgac tactttccgg cgttctccgg cagggtttgc accttgctgt
     2581 gcttctggcc gt
//
LOCUS       DATMZQ010000009        18206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3111824, whole genome shotgun sequence.
ACCESSION   DATMZQ010000009 DATMZQ010000000
VERSION     DATMZQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3111824"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VN496_00305"
     CDS             <1..1540
                     /locus_tag="VN496_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXF15409.1"
                     /translation="LGSFSLGKNDTLNNSYFMFNGGSGYNQLWWFDEYDKLDLGKTIG
                     SYKVTSISGVNIYWREFEKGYVYVNPTATNVSAITLPQASRQITHDTLFSTLSSLPTV
                     NTISLRAHNAAFLTKTTASSVDTIAPSVPIGLTATAVSGTQVNLAWQPSTDNVAVKGY
                     YIYLNDVALTTITGTSFQHTGLTAGTTYSYRVSAFDAVPNNSAWTATPVTVKTGTVTP
                     TPTPTPTTDTVKPSVPTGLTATVVSGTQIKLAWQPSTDNVGVKGYYIYLNDVALTTTT
                     ATSFQHTGLTPGTTYNYRVSAFDAVPNHSAWTATPVTAKTGTAPTPTTDTVKPSVPAG
                     LTATAVSPSQINLKWSSSTDNIGVTGYRVYRAGTLIATVGAVTSYQNTGLTAATLYTY
                     TVTAIDAAGNASAQSIGTWATTQAVQSADTTAPSVPTGLTATVVSSTQINLSWQRSTD
                     NVGVKGYYVYLNNVALTTTSGTSIQLTGLKPGTTYNYRVSAYDAVPNHSAWTATPVSV
                     ATPN"
     gene            1955..2413
                     /gene="gspG"
                     /locus_tag="VN496_00310"
     CDS             1955..2413
                     /gene="gspG"
                     /locus_tag="VN496_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXF15410.1"
                     /translation="MLLAGLPATRFRALRPGFNPVRGFTLLELLVVMVIIGLLAGYVG
                     PRYFAQVGKSEVKTARAQIDALEKALDQYRMDVGHYPSNEQGLDALFVRPESESKWQG
                     PYLKKKPPADPWGNKYFYKIPGEHGDYDLLSYGRDGKPGGSAEDADITNW"
     gene            2463..3653
                     /locus_tag="VN496_00315"
     CDS             2463..3653
                     /locus_tag="VN496_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXF15411.1"
                     /translation="MKFQIKALSDSGGVVLLALDAADEADADRQVRARGCNVLSLRRS
                     NSWSLERFTSSHRFPLALFNQELLALLGAGLTLVESIEALAEKEAQSSTRTTLDRLIE
                     QLRHGRTLSQAMQEQPDAFPVLYVSTVRATERTGDLTHALQRYVAYESQLEVVRKKVI
                     SALIYPVLLMAAGSLVLFFLLGYVIPRFSMVYDEVKGNLPLVSRLLLQLGTFISAHGA
                     MVVLGGLGAIAVLGRVMFTRTTRRWIGAKLWRVPAIGRRMRLYQLARFYRTLGMLLRG
                     GTAAVAALDMAAGLLSPTLRLSAERAREAIREGRMISEAMELHGLTTPVALRMLRVGE
                     RSGRMDDMMDRIAAFLDEEIARWVDWFTRLFEPLLMAFIGLVIGAIVILMYMPVFELA
                     GSIQ"
     gene            3650..5347
                     /locus_tag="VN496_00320"
     CDS             3650..5347
                     /locus_tag="VN496_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXF15412.1"
                     /translation="MNAAIETTFPHVLVTIEQIDQARKLARTTKRSIVQLLEEQLELP
                     PAEFTAALAHTLNMTALTNRRLHALKPAFDVLPFAEANAHECTLLRDEAGSFVMAIGD
                     PFALDLQAWAAERIAEPFTFSLAHRDDIAAYLSQVEEDMHAMDGVIAPVPTGMDTAQA
                     VEDLSLKAISENTSPIVRLVNSTLYDALKTGASDIHLESTGSGLTIRSRIDGVLNPAG
                     TVQGNDLAEQVISRVKVMAELDIAERRIPQDGRFKVTVKGRDVDLRVSIMPSIFGEDA
                     VLRILDKKTLADEFRGLRVDVLGFDSATVGWLRKLANEPYGMLLVTGPTGSGKTTTLY
                     AMLSEIHSGHEKIITIEDPVEYQLPGILQIPVNEKKGLTFARGLRSILRHDPDKIMVG
                     EIRDPETAQIAIQSALTGHLVFTTVHANNVFDVIGRFLHMGVDPYSFVSALNGVAAQR
                     LIRINCPQCSTARPPAPELLAASGLDSTQTADWTFRAGRGCGQCRGTGYQGRKAIAET
                     LTLNDEMRELISTRQPTRAVKEAARALGTRTLREAALELLRHGETNLDEVNRVTFIA"
     gene            5328..6152
                     /locus_tag="VN496_00325"
     CDS             5328..6152
                     /locus_tag="VN496_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15413.1"
                     /translation="MSRLSPEHWQIALSPHQVAMVRFSRARKTSRVTERKLLPCSRTG
                     EQQQSNWSAPLAMLRDNLPQTGANATPVTVILSNHFLRYMVLPWSPELVTQSEELDYA
                     NARFAQVFGDKARQWVIRTSDAPAGAERVGAAADASLLEALSLAFNAGGLTLKSCQPA
                     LMAQFNACRTRIGDNAWLVSAERGRLLVAWIFKQRWRSVRMRSLNDRVVPLRDILEQE
                     RLLLSANEPRYKVFVSAADDIVIDTKGLRPEQVAPGLTSPKASADPEYALAMAGLQ"
     gene            6149..6688
                     /locus_tag="VN496_00330"
     CDS             6149..6688
                     /locus_tag="VN496_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15414.1"
                     /translation="MMYPLQLDFVRSSARQRASGMLLLIAGAIAVCLVSYWRVDLHAK
                     TDAFESRVDRSERETIGLAPIDARLDDSVGREIQRANEVIDQLALPWNRLFRAVESAS
                     AGPVTLLGIAPDAKSGTVQISAETTDAEAMFGYVKRLEQQPELANVYLVEHQRNKRGD
                     VRSLRFLVTASWLATQTRR"
     gene            6661..7221
                     /locus_tag="VN496_00335"
     CDS             6661..7221
                     /locus_tag="VN496_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15415.1"
                     /translation="MARNANPALIWRIKWLARRLDWRAVAGVLLGVLALALYSYSIKP
                     LETRLLAAGERWRQLQVRTNTLPKNAVPAQPTAQLADFYGRLPEARQAPEIAAKLHTY
                     ARNSGLTLERGEYRPQQDALGRFIRYQIVLPVNGSYPQVRRFLGEAMRDLPGLALDGI
                     DFSREAAGSAQLQAELRFTAFLRKPE"
     gene            7218..7772
                     /locus_tag="VN496_00340"
     CDS             7218..7772
                     /locus_tag="VN496_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15416.1"
                     /translation="MTPRKRWFIIAGLLVSTIVAAAWVHNAPEDEGADIVAVVPDKVA
                     SQSASTASRPGTRGENKTPLSARTPQVNVDKLLARDPGALVRDPFAVPAPKIRKPVRG
                     TSAARAPAPAPAAPPMPFTYMGKLHSGPDTSVFLTQGDRSLVVHEGDTIDSIYRVERI
                     ADDEITLVYLPLEERQKILIGESQ"
     gene            7769..10006
                     /locus_tag="VN496_00345"
     CDS             7769..10006
                     /locus_tag="VN496_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXF15417.1"
                     /translation="MRRIVCCLLLLLCAGCAADTALNEGRRLIDQGSMEEGIRHLEKG
                     NKAHPEDPVLRSYYLRYRDAYVDQLNYEGDKARLLGHGEQALAAYGRALEFNPDNARA
                     RAGLDATKAGERQRETVRQAQALSDAGNDEQALSLLKTVLQDNPSQREAQALQRRIEQ
                     NRASKKTAEPQLRPEFRKPVTLEFRDALLKNVFEVLSRVSGINFVFDRDVRADLRTTI
                     MVRDTPLEEALQFLLVTNQLDKKILNQNTILIYPNQPNKLRDYQELVTRSFYLGNADV
                     KQTLNLVKTVLKTRDVFIDERLNLLVMRDTPDAIRLAEKLIAAQDLAEPEVILEMKVL
                     EITRSKLQNLGANYPNRIAAGVQGAAGVPGQITLNEWLNRSSSLAVINITDPAFIINL
                     QQQDSDTNLLANPRIRVRNREKAKIHIGDRLPVITTTSTANVGVSESVQYLDIGLKLD
                     IEPNVYLDDEVAMKVGLEVSNIVQQIQSAQGTLTYRLGTRNTATTLRVANGETQVLAG
                     LIQDEERRTADKVPGLANIPLLGRLFTNNNDARTKTEIVLLITPYVVRNVDRPPPSAL
                     EVASGTEGGFGVAPLRLSTADGLPAPAMDNTPATASRPAQEYATAQPPAATGTAQAQT
                     AAAQPAPAPPDAGKTLALSAPSQVRAGSDFAVAVSMPPGTSGSVRLDLVYDSAKLLAL
                     GAEGTPGRVSINVAGPTTVRFRALEGQSGSAQIAVGSIIPAAGANAAAAPISAPAPLT
                     IDIMR"
     gene            10006..10509
                     /locus_tag="VN496_00350"
     CDS             10006..10509
                     /locus_tag="VN496_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXF15418.1"
                     /translation="MKTPTTSKLSWCAPGAGFTLIELVVTVAIVAILASGAMPLTHVA
                     AQRAKEQDLRAGLRQLRQAIDSYKDAADNGQIEKKADQSGYPPNLRVLAYGVDDIKSV
                     EKHKIYFLRRLPRDPFASDTEKAEDSWMTRSYDSPPDSPREGKDVFDVFSRSEAVGLN
                     GIPYKQW"
     gene            10503..10934
                     /locus_tag="VN496_00355"
     CDS             10503..10934
                     /locus_tag="VN496_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXF15419.1"
                     /translation="MVSNKLSSVADRNRNSGQAGASRVGFTLIELLVVMAIIALLLTI
                     ALPRYWHSTDKAKEAVLKEDLAQMRIAIDQYHADRGKYPDRLEDLVERKYLRSVPRDP
                     VTESTSTWVVVPPSETTAGTVYDIKSGAPGTASDGRNYSEW"
     gene            10960..11553
                     /locus_tag="VN496_00360"
     CDS             10960..11553
                     /locus_tag="VN496_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXF15420.1"
                     /translation="MKPGSSTHTWNSGFTYIALLVLLAIMGIGLAATGIVFHHQAQRE
                     KEKQLLFVGEQIRHAIGRYYENSPGGGKQFPQTLENLLRDDRHPGTHRYLRQVYRDPM
                     TNAKDWVLLRAPDGGIMGVHSALAQKPIKRDGFPSGYEHFADKESYEDWIFQYAVESS
                     VEPSAPGDVPRVPQRGGVPPIPPPGMIGAPPPPAKSK"
     gene            complement(11683..11853)
                     /locus_tag="VN496_00365"
     CDS             complement(11683..11853)
                     /locus_tag="VN496_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15421.1"
                     /translation="MQEPAPLCTQQASGSRMQGRACSVVLQGVLVNTAKQVQRALLKS
                     CRDVAVVRKPGA"
     gene            12039..12842
                     /locus_tag="VN496_00370"
     CDS             12039..12842
                     /locus_tag="VN496_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HXF15422.1"
                     /translation="MAQNSESGSLRFSTAAPLSPETVAPFAADAVEVVVSRLDLEADA
                     FNVLAQWLRNDELQRASRFQFTRDRRRFIVARGCLRQLLAARLGIDPQAVELSYGPNG
                     KPVLAGGSAAMDWRFNISHSEDVAVFAFCRGREVGVDIEAVHSVKNSDAIASQFFSPG
                     ELAAYRALAPHEKDIGFFNCWTRKEAFVKALGGRLDFPLNEFDVSLSPDEPARIIRVG
                     NIAGDDSEWLVRSFAPMPGYVAAVVVQRSRAAANDDAFMKGPACTLWTH"
     gene            12830..14026
                     /locus_tag="VN496_00375"
     CDS             12830..14026
                     /locus_tag="VN496_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF15423.1"
                     /translation="MDTLTRFPNDRRQAVVPSGTYQSPWVDDDVRIFRKTAYQFIEKE
                     FTPNYARWCAQHYPDARAWTRAGETGLLLPDIPEAYGGGGGTFAHVIVVQEELAQAGI
                     QFGCSIQSIVGHYILAYGTEEQKRNWLPRMASGELVAAIAMTEPTAGSDLQGIKTKAR
                     RDGNHYVINGSKTFITNGWHAGLVCVAVKTDSASAGFKGLSLVMVETTNLSGYHVGKP
                     LEKVGMHAQDTCEIFFDETRIPVSNLLGAVEGRGFSQMMEQLPYERLTVAACAVATIE
                     RAVAITSRYVKERSASSKPLIDFQNTRFQLAQCMTEAHVGRVFLDSCIERFIAGELDD
                     ATTAMAKYWLTECQCRIVDQCVQLHGGYGYMMEYPIARMWIDSRAQRIYAGTNEIMKE
                     LIAWSL"
     gene            14060..16888
                     /locus_tag="VN496_00380"
     CDS             14060..16888
                     /locus_tag="VN496_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXF15424.1"
                     /translation="MKWKNTQFGSTTKRGSLLRLFTSAGPETRHIEGGVPAHDPARQL
                     CLHQLIEQQASRTPDAMALVFEQQRLTYHDLVNRASRLAHYLRGQGAGPDKVIGVFME
                     RSLDLVVAILGILKSGAAYLPIDPSFPQERLGFIIDDANVALVLTQASLASVLPVKNL
                     QAVCLDSFDWTTPDSRADQVQMHPDNLAYVIYTSGSTGLPKGVCIEHRNIVNYVLGVA
                     ERFNLVPGMNYATVSTIAADLGNTVIFPSLATGGCLHVIAQERVENQAMLADYFSREN
                     IDVLKIVPSHLAALQTGKNPELVMPRRLLILGGEASRLAWLEKLRELMPECQIHNHYG
                     PTETTVGVLTCHAESKIAATQSGTVPLGKPLPNSRAYILDGNGQPVSPGNRGELFIGG
                     NGVARGYLKRPELTAEKFLRDPFSADPGARMYRTGDLARSLPDGNIEFCGRMDDQIKL
                     RGYRVELGEIECALREDAGIKDAVALAPEIESGTRQLVAYVIPKRTDQPLWSFGQVHL
                     LPDGAPVAHLNKSETDYIYNEIFVLQAYLRHGISIRDGDCIVDAGSNIGLFTVFANRL
                     ARNLRMLSFEPNPAAYACLKANAEAWGSAVKCLPFGLSRENKSAELTFFQGLSLLSGF
                     YADAQTEREVVKTYVLNQQEPDANYRETAAADIGALIDERLQSRIVTAQLRTLSSVMV
                     EEGIEHIDLLKINVEKSELDVLMGIAPNDWPRIRQLVIEVDQQGSLSPITTLLEQQGY
                     EYLVEQDPLLRNTDLCYVYAIRPSQYGRLSRQESVDGHVRALPAVDEEILTPATVRKH
                     LKARLPQYMVPSAFVLMDKFPLNANGKIDRKAFPAFSGGKTSAANMPVALQTETEKAL
                     AAIWSELLGVENIGLHDDFFDLGGHSLLAIRAVARTRDKFEVNLSLRNLFDAPTLEGL
                     ARVIDGLSWLKRSEGLGTGAGNREEIAL"
     gene            16885..17886
                     /locus_tag="VN496_00385"
     CDS             16885..17886
                     /locus_tag="VN496_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HXF15425.1"
                     /translation="MNLQTFLADLYDREIELWADGDRLRCKAPAGVLTTTLRNELMER
                     KSEILAFLRTAEAAAKQPRAIVPLQPHGTRPPVFAVAGHNGDVFTYRTFAAELGNDQP
                     FFGLEPPGLLDGDSEPMDRVEDLAAYFAEQIRTLRPDGPFVIAGYCAGGAIAFELARQ
                     LKQQGASILFLAMLAAPYPSYCRFSTQISEALALRAQSVRNHLRTLTKLSLHDGRQYL
                     VNKLNQRKLRRQAQRVHFNDPVLRRRDRVANVTVAAVARYTPGTYDGRVSLFLPNKEW
                     IYSRAEPLRWRVVAPRAEQHFVPEDCNPDILLLEPDVAATAELFRRCRDTSAMEMAS"
     gene            17901..>18206
                     /locus_tag="VN496_00390"
     CDS             17901..>18206
                     /locus_tag="VN496_00390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF15426.1"
                     /translation="MAAALFLISSLAIGGSERKIVRVANALRQSGHDIHLAYLGAPHT
                     LRSELDRELPVVHLNRSGKFSLPALARLKRYIAIHEISHVGCVNLYPLLYARAAIWQL
                     "
BASE COUNT         3957 a         5276 c         5267 g         3706 t
ORIGIN      
        1 gctgggctct ttttcccttg gcaagaacga caccctgaac aacagctact tcatgttcaa
       61 cggtggcagc ggctacaacc agctctggtg gttcgatgaa tacgacaagc tcgatctggg
      121 caaaaccatc ggaagttaca aggtaaccag catcagcggc gtaaacatct actggcgcga
      181 atttgaaaag ggctatgtat atgtcaatcc caccgccacc aatgtgtcag cgatcacgct
      241 gccgcaggct tctcgacaga tcacccacga caccttgttc tccacactaa gctcgcttcc
      301 taccgtgaat accatttcat tgcgcgccca caatgccgcg tttttgacga aaaccaccgc
      361 gtccagtgtt gatacgattg cgcccagtgt tccgatcggc ctcactgcaa ctgcagtgtc
      421 cggtactcaa gtcaacctgg cgtggcaacc gtccactgac aatgtcgcgg tcaagggcta
      481 ttacatttat ctcaatgacg ttgccttgac tacgatcacc gggacttcct tccagcacac
      541 gggtctgacg gcgggcacaa cttacagtta tcgcgtgagt gcattcgatg cagtgccgaa
      601 taattcggcg tggaccgcca caccggtcac ggtgaaaacc gggacggtta cgccaacgcc
      661 gaccccaacg cctacgaccg acacagtgaa gccgagcgtt ccgaccggcc tgactgccac
      721 cgtggtgtca ggtacgcaaa tcaaactggc ctggcagccc tccacggata atgtcggggt
      781 aaagggctat tacatttatc tcaacgatgt tgcgttgacg accaccacgg cgacttcgtt
      841 tcagcacacc ggtctgacgc ctgggacaac gtataactac cgtgtaagcg cattcgacgc
      901 cgtgccaaat cattcggcgt ggacggccac accggtcacg gccaaaaccg ggacggcgcc
      961 gacgcccacc acggacaccg tgaagcccag cgttcctgcc gggctgaccg ccaccgccgt
     1021 ttcaccctcc cagatcaacc tcaagtggtc ttcgtccacg gacaacatcg gcgtaacagg
     1081 ctaccgcgtg tatcgtgccg ggaccttgat cgcaaccgta ggtgcggtga cctcctatca
     1141 gaacaccggc ttaaccgccg ctactctgta cacctatacg gttacggcta tcgatgcggc
     1201 tggcaatgct tcggctcagt ccatcggcac atgggcaacg acgcaggccg ttcaatccgc
     1261 cgatacgact gcgccgagtg tgccgacggg gctgaccgca accgtggttt cctcaacgca
     1321 gatcaatctc agctggcagc gttcgacaga taacgtcggg gtgaagggat actacgtgta
     1381 tctcaacaac gttgcgctga caactacgtc cggcacatca atacaactga cgggactcaa
     1441 gccaggaacc acatacaact accgcgtaag tgcatacgat gcagtaccga accattcggc
     1501 gtggaccgca acaccggtat ccgtcgcaac gcctaactaa cgggacatca gaacagtgct
     1561 gcgcaagttc aatcttgtgc acacaggtcg actgcaaaat tagggggagg gttggccggg
     1621 tgggaagatg tttttcccgt tcggaccgag cccttcccgt tttcttttat ccactcggcc
     1681 atagctcgcc ggtcgatggt tggaggtgcc ttcgtgagct actacgcacc atggtgcagc
     1741 cctctttgaa cttctgcgtc tcaggtaatt gcctgagttc atcaatcggc cataaaggtt
     1801 atcctgccaa cccgaagttg attcccctta gcaagcggct taacccggtc agtgcggcgg
     1861 tagtggccgg gacgtaatca agcgcaagcc gcaaggcccg ggcagtgcag ttatcaagct
     1921 tggacctgcc atcagagacc tccatggagc cagaatgttg ttagccggat tacccgctac
     1981 acgatttcgt gcattgagac cgggattcaa tcctgtgcgc ggcttcactt tgctcgagct
     2041 gctggtggta atggtcatca tcgggctgct cgcgggctat gtcggcccca ggtacttcgc
     2101 ccaggttggc aagtcagaag taaaaacggc gcgcgcgcag atcgacgcac tggagaaggc
     2161 gctggaccaa tatcgcatgg atgttggcca ctatccatcc aacgaacagg gtctcgacgc
     2221 attattcgtc cgtcccgaga gtgagtcgaa gtggcagggt ccatacctca aaaagaaacc
     2281 gccagcggac ccgtggggca acaaatactt ctacaagata ccgggagaac acggtgatta
     2341 cgatcttctt tcctatggac gcgacggaaa gccgggcgga agcgcagagg acgcggatat
     2401 caccaattgg taagacgaac gacaactgtt ctgcagaact ttgacgaagc gttgccgcaa
     2461 caatgaaatt tcagataaag gcactaagcg attctggagg cgtggtcttg cttgcactcg
     2521 atgcagcgga tgaagcggat gcggatcgcc aggtcagagc gcgtggttgc aacgttcttt
     2581 cgctgcgacg ctcgaattcc tggagcctgg aacgctttac gtcgagtcat cgtttcccgc
     2641 ttgcgctgtt caatcaggaa ctgctcgcgc tgctcggtgc aggactgacc ctggtcgagt
     2701 cgatagaggc gcttgccgaa aaagaggcgc aaagttccac tcgaacgacg ctcgaccgat
     2761 tgattgaaca gcttcgccac ggacgtacct tgtctcaagc gatgcaggaa cagcctgacg
     2821 cctttccggt cctttatgtc tcgacggttc gcgcaaccga gcgcacggga gacttgacgc
     2881 atgcgctgca acgatatgtc gcatacgagt cgcagcttga agtcgtgcgc aaaaaagtaa
     2941 tcagcgcgtt gatctatccg gtattgctca tggccgccgg ctctctggtc ctgttttttc
     3001 tgctcggtta tgtgatcccg agatttagca tggtttacga tgaagtcaaa ggcaacctgc
     3061 ctttggtgtc tcggcttctg ctgcagctcg gaaccttcat atccgcccat ggagcaatgg
     3121 tggttttggg cggactgggt gccatcgcag tattgggccg ggtcatgttc acacgcacca
     3181 cgcgacgctg gataggcgcg aaactctggc gggttcccgc catcggccgc cgcatgcggc
     3241 tctaccagtt ggcgcgcttc tatcgcacgc tcggcatgct gttgcgcggt ggcactgcag
     3301 cagtggcggc cctcgacatg gctgcgggtc tgctttcgcc cacattgcgc ttgagcgctg
     3361 agcgcgcacg cgaagccata cgcgaaggcc gcatgatctc ggaagcgatg gaactgcacg
     3421 ggctcacgac accggtcgcg cttcgaatgt tgcgcgtggg agaacggagt gggcgcatgg
     3481 atgacatgat ggaccggatc gctgcgtttc tcgacgagga gatcgcacgc tgggtagatt
     3541 ggttcacgcg actgttcgag ccgttgctga tggccttcat cggcctggtg atcggtgcca
     3601 tcgtcatact gatgtacatg ccggtctttg aattggccgg gagcatccaa tgaatgccgc
     3661 aatcgaaact accttcccgc atgtcctcgt taccattgag cagatcgatc aggcgcgcaa
     3721 gcttgcacgg accaccaagc gcagcatcgt gcagttgctc gaggagcagc tggaattgcc
     3781 cccagccgag ttcacggcag cgcttgcgca cacgctgaac atgaccgcgc tcaccaacag
     3841 gcggctgcac gcgctgaagc cggcgtttga tgtattgccg tttgccgaag ccaacgcgca
     3901 cgaatgtacc ctgcttcgag acgaagcagg aagcttcgtc atggcgatcg gtgacccatt
     3961 cgcgcttgat ctgcaggcgt gggcggcgga gcgcatagcc gaacctttca ctttcagcct
     4021 ggcccaccga gatgacatag ccgcgtattt gtctcaagtt gaagaagaca tgcacgccat
     4081 ggatggcgtg atagcgccag tgccaaccgg catggacact gcgcaggcag tggaagattt
     4141 gtcgctgaaa gccatcagtg aaaataccag tcccatcgta aggcttgtta actccaccct
     4201 ctacgacgct ctcaagaccg gcgccagcga tatccatctg gagtcgaccg gcagcgggct
     4261 caccattcga agcagaatcg acggagtgct caatccggcc ggcaccgtac agggaaatga
     4321 cttggccgag caggttatat cgcgcgtcaa ggttatggcg gagcttgata ttgcagaacg
     4381 ccgcataccg caggatggcc gcttcaaggt aacggtaaaa ggccgggacg tggatctgcg
     4441 agtgtcaatc atgccgagca ttttcggtga agacgctgtg ctacgtattc tcgacaagaa
     4501 aacactcgcg gatgaattcc gcggtttgcg ggtggacgtg ctgggtttcg atagtgcgac
     4561 ggtgggttgg ctgcggaagc ttgccaacga gccctacggc atgcttttgg tgaccggtcc
     4621 caccggcagc ggcaaaacca ccacgctgta cgcaatgctg tccgaaatcc acagcgggca
     4681 cgaaaaaata atcaccattg aggatccggt cgaataccaa ctgccgggaa ttctgcagat
     4741 cccggtgaac gagaagaaag gactcacctt cgcccgcggc ttgcgttcga tcctgcgcca
     4801 cgacccggac aaaatcatgg ttggcgaaat tcgggatccg gaaacggccc agattgcgat
     4861 tcagtccgca ctcaccgggc acttggtgtt caccactgtg cacgccaata acgtttttga
     4921 tgtcattggg cgtttcctgc acatgggagt agacccttac agcttcgtca gcgcattaaa
     4981 cggcgtcgcc gcgcaaaggc tgatccggat caactgcccc cagtgctcca ccgcaagacc
     5041 gcccgcgccg gagttgctgg ccgcatccgg tctggattcc acgcagacgg cagactggac
     5101 ctttcgtgcc ggcaggggat gtggccaatg tcggggcacc ggttaccagg gacgcaaggc
     5161 gattgccgaa acgcttacgc tgaacgacga aatgcgtgag ctcataagca cgcgccagcc
     5221 gactcgcgct gtaaaggaag ctgcacgcgc gctcgggacg cgcactttgc gtgaagcagc
     5281 actcgagctg ctccgccatg gcgaaaccaa tctggacgag gttaaccgtg tcacgtttat
     5341 cgcctgaaca ttggcagatc gcactgtctc cgcatcaagt cgcaatggtg cggttttcgc
     5401 gggcccgaaa aacttcacgc gttaccgaac gcaagcttct gccctgctcc cgcacgggtg
     5461 agcagcaaca gtcgaattgg agcgcgccgc tggctatgct gcgagacaat ctcccgcaga
     5521 ccggtgcgaa cgcgacaccg gtaaccgtga ttctttccaa ccatttcttg cgctatatgg
     5581 tgctgccatg gagccctgaa ttggtcacgc aatccgaaga actggactat gcgaacgcgc
     5641 gctttgcgca agtctttggc gacaaggctc gccagtgggt tatccgcacc agcgatgctc
     5701 ccgctggcgc ggagcgcgtc ggagcggccg ccgatgcctc gctgctggag gcattatcgc
     5761 ttgcatttaa tgcgggcggg ctgacgctga agtcttgcca gcccgcgctg atggcgcagt
     5821 tcaatgcctg ccgcacgcgc atcggcgata acgcctggct ggtcagcgca gagcgaggcc
     5881 gacttttggt ggcgtggatt ttcaagcagc gatggcgatc cgtgcgcatg cgctcgctca
     5941 atgaccgggt tgttcccctg cgcgatatcc tcgagcagga gcgtctgctg ttgtcggcga
     6001 acgagccgcg ttacaaagta ttcgttagcg cggctgatga catcgtaatc gataccaaag
     6061 ggctgcggcc ggaacaggtt gcaccgggat tgaccagccc caaagcatct gccgatcctg
     6121 agtacgcgct ggctatggca gggctgcagt gatgtacccg ctgcaattgg atttcgtgcg
     6181 gtcctccgcg cggcaacgcg caagcggcat gctgctcctg atcgcgggcg ccatcgcagt
     6241 atgcctcgtc agctactggc gtgtagatct gcacgcgaaa acagatgcat tcgagtcgcg
     6301 cgtggaccgg tcggagcgcg aaacaatcgg tttggcgccg atcgacgcgc gtctcgatga
     6361 ttctgtcgga cgggaaattc agcgagccaa cgaggtcatc gaccaactcg ctttgccatg
     6421 gaaccggctg tttcgcgcag tcgaaagcgc ttccgcaggc ccggtcacct tgctgggaat
     6481 tgcaccggat gccaagagcg gtacggtgca gatcagtgcg gagacgaccg acgcggaggc
     6541 gatgtttgga tacgtcaaac gcctagagca gcagccggaa ctggcgaacg tttatcttgt
     6601 ggaacaccag cgcaacaagc gcggcgacgt tcgctcgctg cgttttctag tgaccgcatc
     6661 atggctcgca acgcaaaccc ggcgttgata tggcgcatca agtggctggc gcgccggctg
     6721 gattggcgcg cagttgccgg tgtgttgctg ggcgtgctcg cgcttgcgct ttattcttac
     6781 agcatcaagc cgctcgagac ccggctattg gcggctgggg agcgttggag gcaattgcag
     6841 gttcgcacca ataccttgcc gaaaaacgct gtaccagcac aaccgacagc gcaacttgcc
     6901 gatttttacg ggcgactgcc ggaagcgagg caggcacccg aaatcgcagc caaattgcac
     6961 acctatgcac gcaactcagg tttgacgctg gagcgcggcg agtaccgtcc gcaacaggac
     7021 gcattgggtc ggttcatccg ctatcagata gtgctgccgg tgaacggaag ctatccgcaa
     7081 gtgcggcgct ttctggggga agcaatgcga gatttgcccg ggcttgcact ggacggcatc
     7141 gatttcagcc gtgaagccgc tggttccgca cagctacagg ctgaattacg tttcactgca
     7201 tttttacgga agccggaatg acgccccgca aacgctggtt cattatcgcc ggtttgctgg
     7261 ttagcacaat tgttgcggcg gcgtgggtgc acaatgcccc ggaggatgaa ggcgcggata
     7321 tcgttgcggt cgttccggat aaggtcgctt cccagtccgc ttccaccgca tctcgtcccg
     7381 ggacacgcgg cgaaaacaaa actccgctct ctgcccgcac tccgcaagtc aatgtggaca
     7441 aactcctcgc acgtgaccct ggagctttgg tgcgcgaccc gtttgccgtg cccgcgccca
     7501 agatcaggaa accggtgcgc ggcacctcag ccgcgagagc gcctgctcct gcaccagcgg
     7561 cgccgcccat gccgttcact tacatgggca agctgcactc gggtcctgat acaagtgttt
     7621 tcctgacgca gggagatcgc agcctggttg tgcatgaagg cgacaccatc gattcgattt
     7681 atcgggttga gcgtatcgcc gacgacgaaa tcacgctggt ctacctgcct ctcgaagagc
     7741 gtcaaaaaat tctcatcgga gagtcgcaat gagacgcatc gtttgctgtc ttttacttct
     7801 gctgtgcgcc ggctgcgcgg ccgataccgc gcttaatgaa ggtcgccgcc tcatcgacca
     7861 gggatcgatg gaagaaggta tccgccacct cgagaaaggc aacaaggcgc atccggagga
     7921 cccggtgctg cgcagctatt atctgcggta tagggacgcc tacgtcgatc agctgaatta
     7981 cgaaggtgac aaggcacgct tgctgggtca tggcgaacag gcgctcgccg cttacggacg
     8041 cgcgttggaa ttcaatcccg ataatgcgcg cgcgcgggcc ggactggacg cgaccaaggc
     8101 cggcgaacgc cagcgcgaaa ccgttcgcca ggcgcaagcg ttgtccgatg ccggcaacga
     8161 cgagcaggca ctgagcttgt tgaaaacagt gctgcaggac aatccatcgc aacgcgaggc
     8221 gcaagccttg cagcggcgta tcgagcagaa ccgcgcgtcg aaaaaaaccg ccgagccgca
     8281 actgcggccc gagttccgca aaccagtaac cctcgagttc cgcgacgcgt tgctcaagaa
     8341 cgtgttcgaa gtattgtccc gcgtttccgg gattaatttc gtgttcgacc gcgatgtgcg
     8401 tgccgacctc cgcacgacca tcatggtgcg agatacaccg ctcgaagagg cgctgcaatt
     8461 cctgctggtt actaatcagc tggataaaaa aatactgaac cagaatacga ttctcatcta
     8521 tccgaaccag ccgaacaagc tacgcgacta ccaggaactg gtgacacgaa gcttttacct
     8581 cggcaacgca gacgtaaagc aaacgctgaa tctcgtgaag acggtgttga agacgcggga
     8641 tgtgttcatc gatgaaaggc tcaacctgct ggtgatgcgc gacacgccgg atgcaattcg
     8701 tctggccgag aaactgatcg ccgcgcagga tctcgctgaa ccggaagtaa tcctggaaat
     8761 gaaggtgctc gagatcacac gcagcaagct gcaaaatctc ggcgccaatt atccaaacag
     8821 aatagcagcg ggcgtccagg gtgcggcagg ggttccgggt cagattaccc tcaatgagtg
     8881 gctcaaccgc agttcgagcc tcgcagtgat taatatcacg gatcccgcct tcatcatcaa
     8941 cctgcagcag caggacagcg ataccaatct gctggccaat ccccgaatca gggtgcggaa
     9001 tcgtgaaaaa gccaaaattc atatcggaga tcggcttccc gtcatcacga cgacttccac
     9061 cgccaacgta ggcgtttccg agtcagtcca atatctggac atcggtttga aactggacat
     9121 cgagccaaac gtttatctcg atgatgaagt ggccatgaag gtcgggctcg aggtcagcaa
     9181 tatcgtccag cagatacagt ctgcacaggg cacgctgacc taccggcttg gcacccgcaa
     9241 taccgcaacg acgctgcgtg tggcgaacgg agaaacgcag gtattggctg gtctcatcca
     9301 ggacgaggaa cggcgtaccg ccgacaaggt tccgggattg gccaatatcc cgctactcgg
     9361 tcgcctcttc acgaacaata acgatgcgcg cactaaaacc gagatcgtcc tgctgataac
     9421 gccgtatgtc gtgcgcaacg tcgacaggcc gcctccgtcc gcgctcgaag tcgcctcggg
     9481 taccgagggc gggtttggag tggcgccgct tcgtttgagc acagccgatg gattacctgc
     9541 gcctgccatg gacaacaccc ctgccacagc gtcgcggccg gcccaggaat atgcgaccgc
     9601 acagccacca gcggcaaccg gcaccgcgca ggcgcagact gccgcagcac aaccggctcc
     9661 cgctccgcct gatgccggca agacactggc gttgtcagca ccgtcgcaag tccgggccgg
     9721 cagcgacttt gcagtcgcgg tgtccatgcc gcctggaaca tccggtagcg tgcggctgga
     9781 tctcgtttat gacagcgcca agctgctggc gcttggcgcc gagggtacgc cggggcgtgt
     9841 ttccataaac gtggcaggac caaccaccgt ccgttttcgc gcactggaag gtcaatccgg
     9901 ctctgcgcaa atagcggtgg gaagcatcat tccggcggcg ggagcaaacg ctgctgcagc
     9961 acccatttca gcgccggcac ctttgacgat tgacattatg cgctgatgaa aacgccgaca
    10021 acttcaaagc tttcctggtg tgcgcctggg gcgggattca cgttgatcga actcgtcgtg
    10081 acggtcgcca tcgtggccat cctggcgtct ggcgccatgc cgctcacgca tgtcgctgcc
    10141 cagcgcgcga aggaacagga cttgcgagca ggtttgcggc aactgaggca ggcaattgat
    10201 agctacaagg acgccgcaga caacgggcag atcgagaaaa aagcggatca atcgggttat
    10261 cctccgaatc tgcgcgttct cgcatacggt gtcgatgaca tcaagagcgt ggaaaagcac
    10321 aaaatctatt ttctgcgcag actgccacgc gatcctttcg catcggacac ggagaaggca
    10381 gaagatagct ggatgacccg ttcgtacgac agcccaccgg acagtccgcg cgagggcaag
    10441 gatgtgtttg atgtcttttc cagatcggag gcagtgggcc tgaacggaat accgtacaag
    10501 caatggtgag caacaaattg tcgagcgtcg cggatcgcaa ccggaattcc ggccaagctg
    10561 gagcttcccg tgtcggcttc acgctcatcg aattgctcgt ggtcatggcg atcatcgcgc
    10621 tgctgttgac catcgcgcta ccgcgctact ggcatagcac cgacaaggcc aaagaggccg
    10681 tgctcaaaga agaccttgcg cagatgcgta ttgcaatcga tcaatatcat gccgaccggg
    10741 gcaagtaccc cgaccgcctg gaggacctgg tcgaaagaaa atacttgcgt tccgttccgc
    10801 gcgatcccgt aacggaaagc acatcgacct gggtcgtagt gcctccatcg gaaacgactg
    10861 cggggaccgt ttacgatatc aagagcggcg ccccggggac ggcgagtgac ggcaggaatt
    10921 acagcgaatg gtaatccgcg cgacgccgtg cgccggaaaa tgaaacccgg atcgagtacg
    10981 cacacgtgga attctggctt cacctatatc gctttgctgg tgttgcttgc catcatggga
    11041 atcggcctgg ctgccaccgg aatcgttttt caccatcagg cgcaacggga gaaggaaaag
    11101 cagctgctgt tcgtcggcga gcagattcgt catgccatcg ggcgctatta tgagaactct
    11161 cccggcggcg gcaagcaatt cccgcagaca ctggaaaacc tgttgcggga tgatcgtcac
    11221 cccggcacgc atcgctacct gcgtcaggtg taccgggatc caatgaccaa cgccaaggac
    11281 tgggtactgc tgcgcgcgcc ggacggcgga ataatgggtg tgcatagtgc tttggcacaa
    11341 aagccgatca aacgggatgg ctttccgtcg ggatacgaac atttcgccga caaggaaagc
    11401 tatgaagact ggatctttca atacgccgtg gaatcatctg tcgagccttc agcgcctgga
    11461 gacgtaccgc gcgtgccgca acgtggaggc gtcccaccga ttcccccgcc cggcatgatt
    11521 ggcgcaccgc ctcctccggc caaatccaaa tagcgacctt ccgaggaatg ccctcgcaag
    11581 tgcggtcgca catcggctat tgaccttgca tacgtttaat cattgagccg tacccggttt
    11641 tccctctacc ggccttggct gaaagcgaac gggctgtctt ggttatgcgc ccggcttgcg
    11701 aaccaccgcg acatcccggc aagacttcag caacgcccgc tgtacctgct tggccgtatt
    11761 gacgagcaca ccttgcagga ccacgctaca agcccgtcct tgcatccggc ttccgctggc
    11821 ttgctgtgtg cacagcggtg ctggctcctg catgcagtgt cgtcagcctg gcgttttgcc
    11881 atgcaattca ttggcgtgag ccaactggat catgcgcacg gcgccgtgaa aaacatgctc
    11941 agcatctttt gacgagtaaa tttgcatcgc gccattcagg aaccgattga gggcgttgtc
    12001 tcgatatccg gacgaacaac aatgcaggtt tccgattaat ggcgcaaaac tctgaatcag
    12061 gttcgctgcg cttttccact gccgcaccgt tgagcccgga aacagtggca ccttttgccg
    12121 ctgatgccgt cgaggtcgtc gtgagccgtc tggatctcga agcggacgca ttcaatgtcc
    12181 ttgcgcagtg gctgcgtaac gacgaactcc agcgtgcaag ccgctttcag tttactcgcg
    12241 atcgtcgccg gttcatcgtg gcgcgaggct gcctccggca attgctggcc gcgcgactgg
    12301 gcatcgatcc acaagccgtc gaactatcgt acggtccaaa cggcaagccc gtcctggcag
    12361 gtggatccgc cgccatggac tggcgtttca atatttcgca ttccgaagat gtcgcagtat
    12421 ttgcgttttg ccgggggcgt gaagtaggcg tcgatatcga agcggtgcat tcggtaaaaa
    12481 acagcgatgc cattgcctcg cagttctttt caccaggcga gcttgcggct tacagggcgc
    12541 tcgctcccca tgaaaaggac atcgggtttt tcaactgctg gacccgcaag gaagcctttg
    12601 taaaggcgct tggcgggcgt cttgatttcc cgttgaatga atttgatgtg tcgttgtcgc
    12661 cggatgaacc agccagaatt attcgtgtcg gaaatatcgc tggcgatgac agcgaatggc
    12721 tagtgcgcag ttttgctccg atgccgggct acgtcgctgc cgttgtcgtc cagcgcagca
    12781 gggccgcggc gaacgatgac gcttttatga aagggccggc ttgtacgcta tggacacact
    12841 gactcgtttc ccaaacgatc ggcgacaggc tgtcgtgccg tccggcacct accaatcacc
    12901 gtgggtcgat gacgatgtgc ggatattccg caagaccgct tatcagttta tcgagaagga
    12961 gttcactccc aactacgcgc gctggtgtgc acagcattac cctgatgcgc gggcatggac
    13021 ccgggcgggc gaaaccggct tgctattacc tgatattcca gaggcatatg gtggcggcgg
    13081 cggcacattt gcacacgtaa tcgtggtgca agaagaactg gcccaggcag gaatccagtt
    13141 tgggtgcagc attcagagca tcgtcgggca ctacattctg gcatacggta ctgaagagca
    13201 aaaacgaaat tggcttccac gcatggcaag cggggagctg gtagccgcca tcgcgatgac
    13261 cgagcctacc gcaggttccg acctgcaggg catcaaaacc aaggcgcgcc gcgacggcaa
    13321 tcattatgtg atcaatgggt caaaaacttt tatcaccaac gggtggcatg ccggcctggt
    13381 ctgtgttgcc gtaaagaccg atagcgccag tgctggcttc aaaggcctgt cactcgtcat
    13441 ggtggaaaca acaaatcttt ctggttatca cgtcggcaaa ccactcgaaa aagtgggcat
    13501 gcatgcacaa gacacctgtg agatcttttt tgatgaaaca cgtattcccg tctccaacct
    13561 gcttggcgcg gtggagggca ggggtttttc gcagatgatg gagcaattgc cttatgagcg
    13621 gctcacagtg gccgcctgcg cagtcgcaac aatcgaacgc gcggttgcca tcaccagccg
    13681 ctacgtcaaa gaacgatccg cctccagcaa accgctcatc gactttcaga atacccgctt
    13741 ccagctggct caatgcatga ccgaagcgca tgttgggcgc gtattcctcg acagctgcat
    13801 cgagcgattc atcgcaggcg aactggacga cgcaaccacc gccatggcga agtactggct
    13861 caccgaatgc caatgccgca ttgttgatca atgcgtgcag ttgcacggcg gctatggata
    13921 catgatggag tacccgatag cccgcatgtg gatcgacagt cgcgcgcagc gcatctacgc
    13981 gggaacgaat gaaatcatga aagagttgat tgcgtggtcg ttgtgaatca ttcaccaatc
    14041 gggagctagc ggaaaaatga tgaagtggaa aaacacgcaa ttcgggtcga caaccaagcg
    14101 cggttcgcta ttgcggttgt tcaccagcgc ggggcccgaa acgagacata tcgaaggcgg
    14161 cgtcccggct catgatccgg caagacagtt gtgcctgcac caattgatcg agcagcaggc
    14221 gtcacgcact ccggatgcca tggcacttgt attcgaacag cagaggctga cgtaccatga
    14281 tctcgtcaat cgcgccagtc ggctcgcgca ctacttgcgc ggccagggcg cgggtcccga
    14341 taaggtgatc ggggtattca tggaacgctc gctcgatctg gtagtggcga ttcttggcat
    14401 cctgaaatcc ggcgcggcct accttccgat cgacccctcg tttccgcagg aacggcttgg
    14461 attcatcatc gacgatgcaa acgtcgcatt ggtgctgact caagcatcat tagcatcggt
    14521 gctgccggtt aaaaatctgc aggcggtttg cctggactcg tttgactgga ccacgccgga
    14581 cagtcgtgcc gatcaagttc agatgcatcc agacaatctt gcttacgtta tttatacgag
    14641 cggctcgact ggcctcccca agggggtatg catcgaacac cgcaatatcg tcaattacgt
    14701 actcggggtt gcggaacgct tcaatcttgt ccccggcatg aattacgcga cggtgtccac
    14761 catcgccgcc gatcttggca ataccgtcat atttccatcg cttgcgaccg gaggttgcct
    14821 gcatgtcatc gctcaggagc gggtggagaa tcaggccatg ctggctgact atttctcgcg
    14881 cgagaatatt gacgtcctca agattgttcc atctcatttg gcggcgctgc aaaccggcaa
    14941 aaacccggaa ctggtcatgc cccggaggct tctcatactg ggcggggaag cctctcgcct
    15001 cgcatggttg gaaaagttgc gggagctaat gccggaatgc cagatacata accactacgg
    15061 ccctacggag accacagtcg gcgttctgac ttgtcacgcg gaatcaaaaa tcgcagcgac
    15121 gcaatcgggc acggtgccgc tgggcaaacc cctgcccaat agccgcgcgt acatcctgga
    15181 cggaaacggc cagcccgtct cccccggcaa tcgaggcgag ctgtttatcg gaggcaacgg
    15241 tgttgcgcgg ggttatctaa agcggcccga gctcacggcg gaaaagttcc tgcgggatcc
    15301 gttcagtgcg gatccgggcg cgcgcatgta tcgcacggga gaccttgcga gaagcttgcc
    15361 agacgggaat attgaattct gcggccggat ggatgatcag atcaagctcc gtggataccg
    15421 tgtagaacta ggcgaaatcg aatgcgcatt gcgtgaagac gctggaatca aggacgccgt
    15481 ggcgctcgcg ccggaaattg aatccggaac gcggcagctg gtggcctatg taatcccgaa
    15541 aagaacagat caaccactct ggagctttgg ccaggtacat cttctgccgg atggcgcgcc
    15601 cgttgcgcat ttgaacaaaa gcgagactga ttacatctat aacgagattt ttgtgctgca
    15661 ggcgtacctg cgccacggaa tatccatacg ggatggcgat tgtattgtcg atgccggctc
    15721 gaatatcggc ctgttcacgg tgtttgccaa caggctcgcc cgcaacttgc gcatgctttc
    15781 ctttgagcct aatcctgctg catatgcgtg tttgaaggcc aatgcggaag cctggggctc
    15841 cgcagtgaaa tgcttgccct ttggcctgtc tcgcgagaat aaatcggcag agctgacgtt
    15901 tttccagggg ctttcgctct tgtccggctt ttacgcagat gcgcaaaccg agcgcgaggt
    15961 tgtcaagact tacgtgctga accagcagga acccgacgcc aattaccggg agaccgcagc
    16021 cgcggacatc ggcgcgctga tagatgagcg gctgcagtcg cgcatcgtga cagcccagct
    16081 acgaacgctg tcgagcgtca tggtggagga aggcatcgag catattgatc tgctcaagat
    16141 caacgtcgaa aaaagtgagc tcgacgtgct gatgggcatc gctccgaacg actggcccag
    16201 aatccgtcag ctcgttatcg aagtagacca gcaaggcagt ctctcgccca tcacaacgct
    16261 tctcgagcag caaggctatg aatatctcgt cgagcaagat ccgcttctgc gcaacacaga
    16321 cctgtgctac gtgtacgcca tcaggccctc gcaatacggc cggctcagcc ggcaagagtc
    16381 cgtcgatggc cacgtgcggg ccttgcccgc ggtggatgaa gaaatcctta cgccggcaac
    16441 tgtgcgcaag cacctcaagg caaggctgcc gcaatacatg gttccgtcgg cttttgtcct
    16501 gatggataaa ttcccgctga atgccaacgg caagatcgac cgcaaggcgt tccctgcttt
    16561 ctccggggga aaaacatccg ctgcaaacat gccggtcgct ctgcagactg aaaccgaaaa
    16621 agcgctggcg gcaatatgga gcgaactgct gggcgtagaa aacattgggc tccatgatga
    16681 cttcttcgat ctcggcggcc actcgctgtt agccatacgt gccgtagcac gcacacgcga
    16741 caaatttgag gtcaatctgt cgctgcgcaa cttgttcgac gcccccacgc tggaaggcct
    16801 cgcccgcgta attgatgggt tgtcctggct caaaagatcc gagggactgg ggactggagc
    16861 gggcaatcgc gaagagattg cgttgtgaac ttacagactt tccttgccga cctgtacgac
    16921 cgggaaatcg agctgtgggc cgatggcgac cgactgcggt gcaaagcgcc ggccggcgtg
    16981 ctgaccacca cgctgcgcaa tgaattgatg gagcgcaaga gcgagatcct ggcgtttctg
    17041 cgcacggccg aggcggctgc caagcaaccg cgtgccatcg ttccactgca gccccatggc
    17101 acgcgaccgc ccgtgtttgc cgttgccgga cacaatggcg acgtctttac ttaccgtact
    17161 ttcgcagcgg agttgggcaa cgaccagccg ttcttcggac tggagccacc gggcctgctc
    17221 gatggagata gcgaacccat ggatcgcgtg gaggatcttg ccgcttattt tgcggaacag
    17281 atccggacac tccgtcctga tggcccattt gtcatcgccg gctattgtgc gggtggtgcg
    17341 attgccttcg agttggcgcg gcaactcaag cagcaaggcg ccagcattct gtttctcgcc
    17401 atgttggctg caccctatcc gtcatattgc cgcttttcca cgcagatcag cgaggcgctc
    17461 gcgctgcgcg cgcaaagtgt gcgcaatcac ctgcgaacgc tgacaaagct ctcgttgcac
    17521 gatggacgcc agtacctggt caataagctc aaccagcgca aattgcgtcg gcaggcgcag
    17581 cgcgtgcatt ttaacgatcc tgttctgcgg cggcgcgatc gcgtcgcaaa tgttaccgtc
    17641 gcggcagtcg cccggtacac accgggaacc tatgatggtc gcgtaagcct gttcctgccc
    17701 aataaagaat ggatttattc ccgtgccgag cctttgcgct ggcgggtcgt agcgccgcgc
    17761 gccgagcagc attttgtacc agaagattgc aatccagaca tcttgctgct cgagcctgac
    17821 gttgcagcca cagctgaact gttccggcgt tgccgggaca caagcgcgat ggaaatggcg
    17881 tcatagttga tttcggaccc atggctgcag cgctgtttct tattagtagc ctggcgatcg
    17941 gaggatcgga acgaaagatc gtccgcgttg ccaacgcatt gcgacagtcc gggcacgaca
    18001 tacatcttgc ctatctgggt gcacctcata cgctgcgatc ggaactagat cgcgaactgc
    18061 ctgtagttca tctaaatcgt agtggcaagt tttcgctccc ggctttggcc cgcctaaagc
    18121 gctatatcgc cattcatgag atatcgcatg tggggtgcgt gaacctttat cccctgctgt
    18181 atgcgagggc tgccatctgg caattg
//
LOCUS       DATMZQ010000010         3552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_353481, whole genome shotgun sequence.
ACCESSION   DATMZQ010000010 DATMZQ010000000
VERSION     DATMZQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353481"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..835)
                     /locus_tag="VN496_00395"
     CDS             complement(137..835)
                     /locus_tag="VN496_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15427.1"
                     /translation="MALLTTRELESFYGDFQALFGIDFEIEQGEIVAMIGANGAGKST
                     FLKTVCGLLKAPAAAVTFDEQPIGGLPPGQIVQRGVAMVPEGRRLFPSLTVTENLLMG
                     AYAKRPGPWTLDAVHALFPVLREKGKSPALALSGGQQQMAAIGRALMSNPKLLICDEL
                     SLGLAPIVVKEIYDALPRICSDGMTAIIVEQDVGMAQRVSNRLYCLQEGRVMLSGSSA
                     ALSREQISRAYFGV"
     gene            complement(931..1653)
                     /locus_tag="VN496_00400"
     CDS             complement(931..1653)
                     /locus_tag="VN496_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15428.1"
                     /translation="MPAILALENVKKSFGALKVTDGISLSVAEGDALGILGPNGAGKT
                     TLFNLISGDLHADSGRVLFRGEDVTLQRPYRRCRSGIGRSYQVPQPFGAMTVFENLVA
                     AASFGTGAREKETYANAVEILRETDLLPKANRLAGTLTLLDRKRLELARALATQPKVL
                     LLDEIAGGLTEHEAVELVEEILRIKKQGMTLIWIEHVVHALLAVADRLFVINSGQKLA
                     EGIPSAVMNDPEVKRVYMGLEA"
     gene            complement(1678..2961)
                     /locus_tag="VN496_00405"
     CDS             complement(1678..2961)
                     /locus_tag="VN496_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF15429.1"
                     /translation="MQSKQFRRRRALIQAGGAFAGTAILGMPMRALAQAKSIRIGFVS
                     PQTGPLAPFGEADNFVIGQMNKLFAGGIDLGGKKVPVVIIQKDSQSSPNRAGEVANDL
                     ILKEKIDLMLVSSTPETTNPVSDACELNEIPCISTVAPWQPWFFGRQGDPGKGFNWTY
                     HVFWGLEDVIGNFLAGWKSVQTNKKVGGLFPNDGDGNAWGDKERGFPGPLAKEGFTLL
                     DPGRFQNMNSDFSAQIAAFKKDNIEIVTGVVIPPDAKTFLTQARQQGFKPKVVTLGKA
                     LLFPGAIEALGDLGDGLSSEVWWSPSHPFTSSLTRQSAKQLADSYEGTTNRQWTQPIG
                     FSHALFEVAVDALKRTKDLSSHAAIRDAVAATSLKTVVGDVKWGGGGPFKNVSKTPLV
                     LGQWVKGSKHKYELVIVNNSQAPAIPAGGKLKLIA"
     gene            complement(3011..>3552)
                     /locus_tag="VN496_00410"
     CDS             complement(3011..>3552)
                     /locus_tag="VN496_00410"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXF15430.1"
                     /translation="LYAQDSNPITSLMAEEGIRALSSSLPTVVSKPDDLEARAEALYG
                     AWLAGACLGNVGMALHHKLCHTVGGAFNLPHAETHAVILPHAVHYNFEAAPDAMARIG
                     RALAGSGLSADGAASGLFALARKLSVPLALRDLGMKMESLDEAARLAMQNPYYNPRPL
                     EYAAIRLLLQNAWEGKRPG"
BASE COUNT          708 a         1086 c         1006 g          752 t
ORIGIN      
        1 atcctttacg gaatcctgcg acgcagggca cggcctttca cttttcgcga tcgtcacgac
       61 cgtcacgtcg tcacggcatt tacggcattc acggcattca cggcattcac gacattcacg
      121 tattcacggc atttttttac accccgaaat aagcgcgtga gatctgctct ctgcttaatg
      181 cagcggacga tccgctcagc atgacgcggc cttcctgcag acagtacagc cggttcgaga
      241 cgcgctgtgc catgccgaca tcctgctcga cgatgatcgc ggtcatgccg tcggagcaga
      301 tgcggggcag tgcatcgtaa atttctttca ccacgatggg agcgagcccg agcgagagct
      361 catcgcagat cagcaatttc ggattactca tcagcgcgcg accgatcgcc gccatttgtt
      421 gctgtccgcc cgacagcgcc agcgctggac tcttgccttt ttcccgcagg actggaaata
      481 gcgcatgaac agcgtcgagc gtccacgggc cgggccgctt cgcgtaggca cccatcagca
      541 ggttttccgt aaccgtcagg ctgggaaaaa gacgccggcc ttcggggacc atggccaccc
      601 cgcgttgcac gatttgcccc gggggaagcc cgccgatggg ctgctcatcg aacgtgactg
      661 cagccgcagg cgctttgagc aacccgcaga ctgttttcag gaaagtcgac ttgccggcgc
      721 cgttggcacc gatcatggct acgatttcgc cttgctcaat ttcgaagtca atgccgaaca
      781 gcgcctggaa gtcgccgtaa aacgattcca gttcgcgtgt cgtcagcagt gccatgcggc
      841 ggggaccaac acacaaagcc tgggcgcaac gcggaccaac gaacgtgaat gacgcgttct
      901 ggcgtaccgg atgcatgcaa ctggtctcat ctaggcttcc aaccccatat aaacgcgctt
      961 cacttcaggg tcgttcatga ctgcagacgg gatcccctct gcgagcttct gtccggagtt
     1021 gatcacgaac aagcgatcgg caacggcaag cagcgcatgc actacgtgct caatccagat
     1081 caatgtcatc ccttgcttct tgatgcggag aatctcttcc accagctcaa ccgcctcatg
     1141 ttcggtgagg ccaccggcga tttcatcgag cagcaatacc ttcggctgcg tcgcgagggc
     1201 acgcgccagc tcaagtcgct tgcgatccag caaggtcagt gttccggcaa gccgatttgc
     1261 cttcggcagc agatcggttt cccgcaagat ttccaccgcg ttggcgtacg tttccttttc
     1321 ccgcgcaccg gtcccaaagg aagctgcggc cacgaggttc tcgaacacgg tcattgcacc
     1381 gaacggctgg ggcacctggt aggagcgtcc tatgccggaa cggcagcgcc gatagggccg
     1441 ctgtaatgtc acatcttccc cgcgaaacag aacacggccg gaatcggcat gcaagtctcc
     1501 gctgatcagg ttgaacagcg tggtcttgcc cgctccgttg ggacctagaa tgccgagcgc
     1561 atcgccctcg gcaaccgaca gcgaaattcc gtcggttacc ttgagcgcgc cgaagctttt
     1621 ttttacgttc tcaagagcga gaatcgcggg catcagactc cccgatattg cggtggatca
     1681 ggcgatcagc ttgagcttgc ctccggcagg aatggcgggc gcttgcgaat tgttcacgat
     1741 gacgagttcg tacttgtgct tgctaccctt gacccactgc ccgagcacga gcggggtctt
     1801 gctgacgttc ttgaacggcc cgccaccacc ccatttcacg tcgccgacta cggttttcag
     1861 gctggtcgct gccacggcat cgcggatggc ggcgtggctg ctcagatcct tcgtacgttt
     1921 caaagcgtcg acggcaacct cgaacagcgc gtgagaaaaa ccaatcggct gggtccactg
     1981 ccgattggta gtcccttcat aggaatcggc aagttgcttc gcactctgcc tggtcagtga
     2041 tgaggtgaac ggatgcgaag gcgaccacca cacttccgac gacaagccgt cgccgagatc
     2101 gccaagcgcc tcgattgcac cggggaaaag caacgccttg ccgagcgtca cgacttttgg
     2161 tttgaagcct tgctgcctgg cctgggtcag gaaagttttt gcgtctggcg ggatgaccac
     2221 tccagtaacg atttcgatgt tgtctttctt gaacgctgcg atttgcgcgg agaaatcact
     2281 gttcatgttc tggaaacgac ccggatcgag caatgtgaaa ccttccttgg caagcggtcc
     2341 ggggaatccg cgctctttgt cgccccatgc gttgccgtcg ccatcatttg gaaacaggcc
     2401 gccgactttc ttgttggttt gcaccgattt ccagccggcc aggaaattgc cgatgacgtc
     2461 ctcgaggccc cagaacacgt gataagtcca attgaaccct ttccccggat ccccctggcg
     2521 accaaagaac cacggttgcc agggtgcgac ggtcgatatg cagggaatct cgtttaattc
     2581 gcaagcgtca gacaccggat tcgtcgtctc cggcgtggac gagaccagca tcagatctat
     2641 tttttctttg aggatgaggt cgttcgccac ttctccggcg cggttcggac tggattggct
     2701 gtctttctga atgatcacga ccggaacctt cttgccgccc aggtcaatgc caccggcaaa
     2761 cagcttgttc atttggccga taacgaaatt gtcggcctca ccgaacggcg ccaatggccc
     2821 cgtctgcgga gatacgaaac caatcctgat acttttagcc tgcgccaatg cacgcatcgg
     2881 catgccgagt atggcggtac cggcaaacgc gccgccggcc tggatcagcg cgcggcggcg
     2941 ccggaattgt ttgctttgca tgaatgctcc tcctgagttt atttatagtt gatgatgtgt
     3001 aagtctgact ctagcccgga cgttttccct cccaggcgtt ttgcaaaagc agtcggatgg
     3061 ccgcgtactc cagcggccgc gggttgtaat aaggattctg catggcgagc cgcgccgctt
     3121 cgtcgagcga ctccattttc atgcccaggt cgcgcaatgc aagtggcacg ctgagcttgc
     3181 gcgccagtgc aaacaggccc gaagccgcgc cgtccgccga caaacctgac ccagccagcg
     3241 cgcgaccgat gcgcgccatt gcgtccggcg ccgcttcgaa gttgtaatgc acggcatgcg
     3301 gaaggatgac ggcatgggtc tcggcatgcg gcagattgaa cgcaccgccg acggtatggc
     3361 agagcttgtg atgcagggcc atgccaacgt tgccaaggca ggcaccggca agccacgctc
     3421 cgtacagcgc ttcagcgcgc gcctccagat cgtccggttt cgacactacc gtcggcaggc
     3481 tggaagacag tgcacggatg ccttcttcgg ccatcagcga cgtgatcgga ttcgagtcct
     3541 gcgcgtacag cg
//
LOCUS       DATMZQ010000011         2630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4837607, whole genome shotgun sequence.
ACCESSION   DATMZQ010000011 DATMZQ010000000
VERSION     DATMZQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4837607"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VN496_00415"
     CDS             complement(<1..1102)
                     /locus_tag="VN496_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXF15431.1"
                     /translation="MRIAIGQINCTVGDLSGNSRKILEFAARAREQGADILVTPELSL
                     CGYPPEDLLLREDFRSACDRALVALAAQVRGITVVVGHPQQVGASLYNAASVLQEGRI
                     AASYHKLDLPNYTVFDEERYFDSDGSPCVVDINGVRVGVNICEDVWGPAGETAARAPN
                     GNPSTVGINICADVWEPEAPKRALAAGAQMLLVLNASPFHIDKLRSRHEVVRERTAET
                     GMPVVFCNLVGGQDELVFDGASFVMDERGNLTHQLPAFEEALAVVDIDRDGPRPGHIA
                     PELDIDASLYKALCMGVRDYLGKNGFREALLGLSGGIDSALTLCIAADAIGADKVHAV
                     MMPSQFTAGISVEDSREMIKRLGVRYSEIAIKP"
     gene            1135..2283
                     /locus_tag="VN496_00420"
     CDS             1135..2283
                     /locus_tag="VN496_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF15432.1"
                     /translation="MKTHSGFQVLDSLATIPASDWDALAGNHPLLSHAFLHALHESGC
                     ASAETGWAPCYLTLWRHGRIAAAMPLYLKSHSYGEYVFDWAWADAYHRHGLRYYPKLL
                     NAVPFTPVTGSRLLAANASDRRALLDAALKFARDSGVSSLHCLFPAPAEVPQFESAGM
                     TLRASVQFHWNNEGFADFDDFLARMNHDKRKKIRQERRKVRDAGIEFRWLRGHEASAV
                     DWRFFVQCYNSTYRAHHSTPYLNLDFFKRLAQAMPQNLLLILGYREGQPIASAFNIIG
                     DNTLYGRYWGTKEFHSGLHFETCYYQAIEYCIAERIGSFEGGAQGEHKLARGLLPQRT
                     VSAHWLAHPEFAEVITRFLRKEADGIEDYVDELSEHSPFKQSEPPASG"
     gene            2325..>2630
                     /locus_tag="VN496_00425"
     CDS             2325..>2630
                     /locus_tag="VN496_00425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXF15433.1"
                     /translation="MTERIAKAVSVASVLMLCVLSPFASHAAPNEIEVTDQVIGTGPE
                     PRRGWFAIIRYTGWIYDEQAADKKGKQFIHSDDRGAPVSFVYGYKRALPGLEKGMEGM
                     "
BASE COUNT          540 a          810 c          754 g          526 t
ORIGIN      
        1 ccggtttgat tgcaatttcg ctgtagcgca cgccaagccg cttgatcatc tctcgtgaat
       61 cttccacgct gatgccggca gtgaattggg aaggcatcat gactgcgtgt actttgtccg
      121 caccgatcgc gtcggctgca atgcacagtg tcagcgccga atcgattcca ccagacaggc
      181 cgagcagggc ttcgcggaaa ccgttcttgc cgagatagtc gcgcacgccc atgcacagcg
      241 ccttgtaaag gctcgcgtcg atatccagtt cgggagcaat gtgaccggga cgtggcccgt
      301 cgcggtcgat atcgaccacg gcaagcgcct cctcaaacgc gggtagctga tgggtcaggt
      361 tgccgcgttc atccatcacg aatgacgcac cgtcgaatac gagttcatcc tgaccgccga
      421 ccagattgca gaatacgacc ggcatgccgg tctctgccgt acgctcgcgc acgacttcat
      481 ggcggctgcg cagcttgtcg atatgaaaag gggaagcatt cagcacgagc agcatctgtg
      541 cgccagccgc gagcgcgcgc ttgggtgcct cgggctccca gacgtccgcg cagatattga
      601 tgcctacagt gctcggattt ccgtttggcg cacgcgctgc tgtttcgccg gccggccccc
      661 acacatcttc gcagatgttc acgcctaccc gcacgccgtt gatatccacg acacacgggc
      721 tgccatctga atcgaaatag cgctcctcgt cgaataccgt gtagttgggt aaatccagtt
      781 tgtgatagct ggccgcgatg cggccttcct gcagcacgga agcggcgttg taaaggctcg
      841 cgccgacctg ctgcggatgg ccgacgacaa ccgtgatgcc gcggacttgt gcggccagcg
      901 caaccaatgc gcgatcgcat gcggaacgaa aatcctcgcg cagcagcaga tcttccggtg
      961 gataaccaca caaggatagt tccggtgtga cgagtatgtc ggcgccctgc tccctggccc
     1021 gtgccgcgaa ctcgaggatc ttgcgggaat tgccggaaag atccccaacc gtgcagttga
     1081 tctggccaat cgcaatgcgc atggctgcta ctataacatc cgctccttgc gacaatgaaa
     1141 acacactccg gctttcaggt cctcgattcg ctcgcaacga ttcccgcttc ggattgggac
     1201 gccttggcgg gaaatcatcc gctgctttcg cacgctttcc tgcacgcgct gcacgaaagc
     1261 ggctgcgcca gcgccgagac cggatgggcg ccgtgctacc tcacgctatg gcggcacggt
     1321 cgcatcgcgg ctgccatgcc gctctatctg aaatcccatt cctacggaga gtacgtcttc
     1381 gactgggcat gggccgacgc ataccaccgg cacggcctgc gctactaccc gaagctgctg
     1441 aacgcggtgc cattcacacc ggtaaccgga tcacggctgc tggccgccaa tgcaagcgat
     1501 cggcgcgcgt tgctcgatgc ggcactgaag tttgcgcgcg attccggggt ctcgtcgctg
     1561 cactgcctgt ttcccgcgcc ggccgaagtc ccgcagttcg agtccgcggg catgaccttg
     1621 cgagcctcgg tgcagttcca ctggaacaac gaaggcttcg ccgactttga tgatttcctc
     1681 gcgcgcatga atcatgacaa acgcaagaaa atccgccagg agcgtcgcaa ggtccgcgat
     1741 gcgggcattg aatttcgctg gctgcgcggc cacgaagctt cggctgtcga ctggcgattc
     1801 ttcgtgcaat gctacaactc gacctaccgg gcccatcatt cgacacctta cctgaacctg
     1861 gatttcttca agcgcctcgc gcaagccatg ccgcagaatt tgttgctgat actgggctat
     1921 cgtgaaggcc aacccatagc gtccgcgttc aacatcattg gcgacaacac gctgtacggg
     1981 cgttactggg gaacgaaaga gtttcactcg ggactgcatt ttgaaacctg ctattaccag
     2041 gccatcgaat actgcatcgc tgaaagaatc ggttcattcg aaggcggcgc tcaaggcgag
     2101 cacaagctcg cgcgcggcct gctgccgcaa cgcacggtat ccgcgcactg gcttgcgcat
     2161 ccggaattcg ccgaagtcat aacacgcttt ctgcgcaagg aagcagatgg cattgaagat
     2221 tacgtcgatg aattgtcgga gcacagtccg ttcaagcagt ccgagccccc ggcttccgga
     2281 tgagcgctgc gggatttggt taaactagca gcatgctgcc cgacatgaca gaacggatcg
     2341 cgaaagcagt ttccgtcgca agcgtattga tgctttgcgt gctgtcacca tttgcgtccc
     2401 atgcggcgcc aaatgaaatc gaggtcaccg accaggtcat cggcaccggt ccggaaccgc
     2461 gacgcggctg gttcgcgatc atccgctata ccggctggat ctatgacgag caagccgcag
     2521 acaagaaggg aaaacaattc atccactcgg acgatcgcgg cgcaccggta tctttcgtat
     2581 acgggtacaa gcgcgcgctg cctggcttgg aaaaaggcat ggaaggcatg
//
LOCUS       DATMZQ010000012         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4147709, whole genome shotgun sequence.
ACCESSION   DATMZQ010000012 DATMZQ010000000
VERSION     DATMZQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147709"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VN496_00430"
     CDS             complement(<1..1166)
                     /locus_tag="VN496_00430"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXF15434.1"
                     /translation="MDISTTPQIIASLLALGIALAFIVSDRQAPTSRALSIFLASIGI
                     SIACFTQFELPLLRGNRYPDWGGIFALPDVCAFYFGYEWLLRIRKTIPTRNLKTRFGD
                     NQLRVAQLLAIGYGLLALAFPRVRAYEFIGSVSGSAIGPGFYLFGIPLVISLGLGIAS
                     GILTLNRRPDVAERQRLVAFLVAAPFMASSMVLPANLSPVLVSFGMLVFLVGGMQYYV
                     IQGRRAQFMSRFLAPQVADLVRDQGMASATREQTLELSVVCCDLRGFTAFAAVTDPGK
                     VIQILREYYDAVGVAAATAGGTIKDQAGDGVLILVGAPIAFDDHAPRSLALAREIRAS
                     GVGMTTRWSDGATQLGIGIGVATGFVSVGVIGTASRLEYAAVGSAVNLASRLCA"
     gene            complement(1211..2080)
                     /gene="ylqF"
                     /locus_tag="VN496_00435"
     CDS             complement(1211..2080)
                     /gene="ylqF"
                     /locus_tag="VN496_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HXF15435.1"
                     /translation="MTIQWFPGHMSIARKKAAETMEWVDVVIEIVDARLPEASSNPMI
                     RTLRNHRQRPALKLLNKADLADPVATRAWLDFYNRQQGINAVALSCKKTADVARVTGY
                     CRTLAPHRSDNTKPLRMMIMGIPNVGKSTLMNALVKRRVAAVGDEPAITKTQQSIDLG
                     PGMTLIDTPGLMWPKIEYESDGYMLAASHAIGTNEVMEEEVAAFLGEILLARYPGPLT
                     ARYGLALDGLDGPSLVEAVATRRGCRKTGGSLDLEKAALILLDDYRSGKLGRISLETP
                     ETRGAMLRHAQET"
     gene            2324..2638
                     /locus_tag="VN496_00440"
     CDS             2324..2638
                     /locus_tag="VN496_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15436.1"
                     /translation="MQEQTDYRRKVEDAVQRATNILAKHHLKPQKYRVHIRSKGDSCA
                     GEPHALSLSTADGALWVTETVIPFRCIEEVDPFGSDAFCSIVSAQMMALIRKVREAGR
                     MV"
     gene            complement(2791..>3459)
                     /locus_tag="VN496_00445"
     CDS             complement(2791..>3459)
                     /locus_tag="VN496_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15437.1"
                     /translation="LVNLAYQVVRKPSELFFPVSDALYKTPPETWKAYGPLFRQHATE
                     VVTPALLAALAQVEGSGNPVARTYWRWSLQRRPFEVYRPASSAVGMYQMTDGTFAAAK
                     RYCVHDHVVVEDGPWNDFASCWFNRFYTRTLSSNAIELTSAYLDRTIANTLTRLRMGN
                     AALGEKQDLAAVIHVCGAGTGEIFVRRRFRLDSGQRCGEHDLQAYVMRVNQMKKVFAR
                     LDGK"
BASE COUNT          754 a         1033 c          964 g          708 t
ORIGIN      
        1 tcggcacaca aacgtgatgc gaggtttacc gccgaaccga ccgccgcgta ttccaggcga
       61 gaggccgtgc caatcacgcc tacagaaacg aatccggtcg ccacgccgat gccgatgcct
      121 aattgcgtcg ccccgtccga ccagcgtgtc gtcatgccaa caccacttgc acggatctcg
      181 cgcgcgagtg ccagcgagcg cggtgcatgg tcatcgaaag cgatcggggc gccgaccagg
      241 atcagtacgc catcgcccgc ctggtccttg attgtcccgc cggcggtggc tgcagcgaca
      301 ccgacggcat cgtagtactc gcgcagaatc tggatcactt tgcccggatc ggtcaccgct
      361 gcaaaagcag taaagccgcg caggtcgcaa cataccaccg aaagttcgag cgtctgctcg
      421 cgcgtcgcac tcgccattcc ttgatcgcgc accaggtctg ccacttgcgg tgccaggaaa
      481 cgggacatga attgtgcgcg gcgaccctgg atgacgtaat actgcatgcc ccccaccagg
      541 aaaaccaaca tgccaaacga gaccaggacc gggctgaggt tcgcaggcaa taccatactc
      601 gacgccatga aaggggcagc aacgagaaaa gcaaccaggc gctgacgctc ggcgacatcc
      661 gggcgccggt tgagcgtcag gattccggat gcaatgccaa gccccagtga aatgacgagc
      721 ggaataccga acaagtagaa tccggggccg attgcgctac ccgaaacgct gccaatgaac
      781 tcatacgcgc gcacacgcgg gaaagccaaa gccagcaagc cgtagccaat ggccagcaac
      841 tgcgccacgc gcaattggtt gtcgccgaat cgggttttca gattacgggt cgggatggtc
      901 ttgcgtatgc gaagcagcca ttcatagccg aaatagaacg cgcacacgtc cgggagcgcg
      961 aaaattccgc cccagtccgg gtagcggttc cctctaagca gcggtaattc gaactgcgtg
     1021 aaacaggcga tggaaatgcc aatggacgct agaaaaatgg acagtgcccg gcttgtgggc
     1081 gcctggcggt cgcttactat gaacgccagc gcaatgccca gtgctaatag cgacgcaata
     1141 atctgcgggg tggtcgagat gtccacgggt ttaatttcag cccagcgaag ctggcataaa
     1201 tactttgcgc tcaggtttcc tgcgcatgtc gtagcatcgc gccgcgtgtc tctggtgtct
     1261 ccagactgat gcggcccagt ttaccgctgc gatagtcgtc gagcaggata agcgccgctt
     1321 tttcgagatc gaggctacca ccggtcttcc ggcagccccg tcgcgtggcc actgcttcga
     1381 ccaggctcgg accgtccagc ccatccagtg ccagaccgta gcgcgcggtc aacggcccgg
     1441 gatagcgcgc gaggagaatt tcccccagga atgcggcgac ttcttcttcc atgacttcgt
     1501 tcgtgccaat tgcatggctg gcggccagca tgtagccgtc gctttcatat tcgatcttcg
     1561 gccacatcaa ccccggcgtg tcgatcagcg tcatgccagg gcctagatcg atgctttgct
     1621 gggttttggt aatggcaggt tcatcgccga ctgctgcgac ccgacgcttg accaatgcat
     1681 tcatcagcgt cgatttacct acattgggaa tgcccatgat catcatgcgc aatggtttgg
     1741 tattgtcgct gcggtgcggc gcaagagtcc tgcaatagcc cgtcacgcgc gcaacgtcgg
     1801 ccgtcttctt gcaagacagg gcgaccgcat tgatgccttg ctgccgatta tagaaatcaa
     1861 gccatgccct ggttgcgacc ggatcagcga ggtccgcttt attcaacagc ttcaatgccg
     1921 gccgctgccg gtgattgcgc aatgtccgga tcatcggatt gctgcttgct tccggtaaac
     1981 gcgcatcgac gatctcgatg accacgtcca cccattccat tgtctccgcg gctttcttgc
     2041 gcgcgatgct catgtggccg gggaaccatt ggattgtcat agttgaaaaa aagacttcaa
     2101 gtcagttgat gcaaccgtaa ggcacggtat agcttcgcct gcatcgcctg caatttgcga
     2161 gctgttttgt cccacgcaca tttttttatg ccagcgtcaa ggcgccttcc gtcgttcgtc
     2221 ggcgacgcgt cgccaaagtc cgacagtttt gcctcttgca cgaatgtgtg ctcaggacta
     2281 aaaagacggt gagggagttg tatcggtacg gaggagtgga ccaatgcagg agcaaacaga
     2341 ttatcgcagg aaggttgagg acgcagttca aagagcaacg aatatcctcg ccaagcatca
     2401 tttgaaacca caaaagtatc gtgtccatat tcgctccaag ggagattcat gcgctggcga
     2461 accgcacgca ttgtcccttt cgacagcaga tggcgcactc tgggtaactg aaacagttat
     2521 tccgtttcgc tgcattgaag aagtggatcc tttcggatcc gacgcattct gcagcatcgt
     2581 atcagcacag atgatggcgc tgatccgtaa ggtaagggaa gccggcagaa tggtctagtt
     2641 tttccaaatg ccctgcctcg gggtttcagc ttcctccctt gctgaatctc ccggtgcagg
     2701 gcatttttcc ctgacgcaca acgggtcccg tctcttccgc cggctagtgt ggccccggat
     2761 tccagcaaat aatcctgtct ccaaagcggt tcacttgccg tccagccgcg cgaatacctt
     2821 cttcatctgg ttcactcgca ttacatacgc ctgaagatcg tgctcgccgc accgctgccc
     2881 ggaatccagc cggaaccggc gccgcacgaa aatttcaccg gttccggcac cgcaaacgtg
     2941 gatcactgca gcaaggtcct gtttctctcc cagggctgcg tttcccatgc gcaagcgcgt
     3001 cagcgtattg gcgatggtgc gatccaggta cgccgacgta agctctattg cattcgagga
     3061 tagcgtccgc gtatagaaac ggttgaacca gcacgacgca aagtcattcc agggtccatc
     3121 ttcaacaaca acatggtcgt ggacgcaata gcgcttggcc gctgcaaacg tcccgtcggt
     3181 catctgatac attccgaccg cgcttgaggc tggccgatag acctcgaaag gcctgcgctg
     3241 caaagaccaa cgccagtaag tgcgcgcgac cggattgccc gacccttcca cctgtgccag
     3301 ggcggccaga agagcaggcg tcacgacttc ggtcgcgtgc tgtcgaaaca gcgggccgta
     3361 agccttccag gtttctggcg gcgtcttgta aagcgcgtcg ctcaccggaa aaaacagttc
     3421 tgaaggcttg cgcacaacct ggtaagccaa gttgaccaa
//
LOCUS       DATMZQ010000013        12379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2426492, whole genome shotgun sequence.
ACCESSION   DATMZQ010000013 DATMZQ010000000
VERSION     DATMZQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426492"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN496_00450"
     CDS             <1..185
                     /locus_tag="VN496_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15438.1"
                     /translation="GHVDRARAEVDAGGVGMEGGQLGIGFRGFWWAERIRWKKEERSG
                     ISMPYEAQGASGIDFQ"
     gene            complement(240..692)
                     /locus_tag="VN496_00455"
     CDS             complement(240..692)
                     /locus_tag="VN496_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15439.1"
                     /translation="MNSKQIAYFQGSLWPAACAAQGWDRNDREFRLEFISSVIGRRIE
                     SAKEVEERDEFTLLKNRCLRLADDLDGAIEDGSLVLNQRRNLLWIIEQLHVPAAVMNH
                     FLRERFHGRILDDLNLVEVQQLLFTLNGWSNRHKRSAKTQNEPVQSPF"
     gene            complement(689..1165)
                     /locus_tag="VN496_00460"
     CDS             complement(689..1165)
                     /locus_tag="VN496_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15440.1"
                     /translation="MSGKSPMFPESETRPVLQVGCVELDRRSLIDEVRSLRAAVERLY
                     LRVKHQSHQAEDLLEVVMELAAVEFARECERWRTWMLSQNRSEPKVTARMVAMKLCVE
                     VLGWGKNYTGRRFKHDRGTVMNACRSIGRRCDVDDRFFKRYQAVAAKARALLPELK"
     gene            complement(1162..1674)
                     /locus_tag="VN496_00465"
     CDS             complement(1162..1674)
                     /locus_tag="VN496_00465"
                     /inference="COORDINATES: protein motif:HMM:NF026141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15441.1"
                     /translation="MTALPFNQALKLAEKIKAELAPFCERIEIAGSIRRRRPFVNDID
                     LVCLPLADQVNALRARVLAKTQPISDGPQTILTRLANGVQLDVWIAQRPFKDMFFNTP
                     TNFGSLLVCRTGSKEHNIYLCQQAEKLGRRWNPHFGVFDNGKCLGSATEEEIFKALDL
                     EFVRPEDRER"
     gene            complement(1671..1958)
                     /locus_tag="VN496_00470"
     CDS             complement(1671..1958)
                     /locus_tag="VN496_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15442.1"
                     /translation="MKIAREAAMAAKPDYKIFWGNTKPEVIAALKREAEAQTRFFKPF
                     SGETPEEFAIRVWACGFLYSREFYAEAVALRQAGWPPDPSPPSPRRGKHSG"
     gene            complement(1955..2908)
                     /locus_tag="VN496_00475"
     CDS             complement(1955..2908)
                     /locus_tag="VN496_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HXF15443.1"
                     /translation="MKREHAIERLRQVIRRKHFSLSTEESYCGWLRRYMLFVRKLRSG
                     LASEQKVEVFLTTLARDDVSASTQNQAFNALIFFYREALGRPLQRVDSLRAKRAAVVR
                     NAPSVQETRALLGGVRDASGYPVRLVVKLIYGCGLRVSEPLNLRIRDVRLDDSRLVIR
                     QGKGRKDRVVAIPCSLAGEIKAQMESARAVWRRDVAAGVPIKLPNQIARKYPHSQFAW
                     PWAWLFPLAAPCADPRTGGIVRWRMLECNVQRAVKRAGRKLGLAILPHELRHAYATHC
                     LNSGQNPRAIQEAMGHRSLETTMGYLHAEALSVRSPLEVLV"
     gene            complement(2929..3267)
                     /locus_tag="VN496_00480"
     CDS             complement(2929..3267)
                     /locus_tag="VN496_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15444.1"
                     /translation="MFKPQFAPLVEAGTKCQTVRPTPKRMPKAGDKISLRMWTGKPYR
                     SQQRVLRESVITAVEPFDLDAMRLWKESDRDAFARADGFGDWPEMLQWFIKTHGYPFV
                     GVTIKWHNKD"
     gene            complement(3381..3686)
                     /locus_tag="VN496_00485"
     CDS             complement(3381..3686)
                     /locus_tag="VN496_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15445.1"
                     /translation="MKLDRNITNPRRGKYALIKLRVATPRIIRDESGDVRVKAESVDF
                     GDTEDSDFFVIRLKDKYAGPALTAYAVEAEQDDPEYAKEIFALADKAKNHPNRKMPD"
     gene            complement(3781..4290)
                     /locus_tag="VN496_00490"
     CDS             complement(3781..4290)
                     /locus_tag="VN496_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15446.1"
                     /translation="MPNSDTFDVPSIAGFVKKYLMKSKISVDCFSRNKRWTTYTNDLN
                     PNTAAEYHMDAEDFLTMLADKDIVADLVICDPPYSPRQVKECYDSIGLPMKQTDALLG
                     MTRKRLKRQINRLVPVGGVVLHFGWNTVGMGKGWNIEEVLLVCHGSDHNDTICMAERK
                     TPELQETFL"
     gene            complement(5690..6223)
                     /locus_tag="VN496_00495"
     CDS             complement(5690..6223)
                     /locus_tag="VN496_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HXF15447.1"
                     /translation="MKSSLAEIERFTKDFADARERLTQLVGDIESELAAVKRRHMAAL
                     KRFVAGTAERHARLKAAIEETPELFVKPRTLILHGVKIGFQKGKGGIEFDNADRVVEL
                     IRKHFDDPDALIRREEKPDKEALAKLSAAELKKIGCAIVDAGDQVVIKPTDGDVEKIV
                     NALLKDSVDEPGVKEAA"
     gene            complement(6220..6417)
                     /locus_tag="VN496_00500"
     CDS             complement(6220..6417)
                     /locus_tag="VN496_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15448.1"
                     /translation="MKNESILLAEAARFRTLAAEAALSALNYVPGDDKKHAENVRLAR
                     EHALRAETCKDAARLIGKGAK"
     gene            complement(7297..7548)
                     /locus_tag="VN496_00505"
     CDS             complement(7297..7548)
                     /locus_tag="VN496_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15449.1"
                     /translation="MSTKKISREGAKGAKPDNYGIPQQVLGLVHTSVDDAHSRLWHWT
                     AADLNDLRKAHELCVYQGQKTKATIIARRIRQLEKGGGR"
     gene            complement(7545..8621)
                     /locus_tag="VN496_00510"
     CDS             complement(7545..8621)
                     /locus_tag="VN496_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF15450.1"
                     /translation="MEDDQKLAVIEKGGAGVINVSGDTCTVKTADYPEEHRNLVRWLF
                     AFAKEMEWSWEDLARETKISTTTMYRVWTGKYRNTDTGELVDLTSLCERIARFKELAE
                     ERALARRLPFIETSVFRRIEKVCREALIMQTIAMIYGESQIGKTESLKEVARRNNHGN
                     TVYVLMPASAGVQAMMKAIAEACHISVRTSFEQLRERVGNYLDGSKLLAIDEIHECFV
                     SYQKSSLVKCLSVLRQLQEISQCGLVLCGTNVFRHELERGEFSQSLKQLRKRGIWELQ
                     LEDAPKEKDLAAIADFYKLPAPGGEAAELVKWIGKEMGLGKYTRFLARGAQLANKRND
                     RFGWKHFVEIVGIATKLKQKQEGQ"
     gene            complement(8633..10639)
                     /locus_tag="VN496_00515"
     CDS             complement(8633..10639)
                     /locus_tag="VN496_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15451.1"
                     /translation="MLPEENSGARSDLSSGPGVSTDPVCVAHETPAAFSIPASAQAEF
                     IALPEPARAQVLSRLKVMQSIAASGNKRATCRFYAARLRGVRGGSAQRFYTLYFAYRK
                     SGDWRVLLDHAKAGIKLTGSACHAALPRAFREFWKSLCEENKRRGGDKAAHRVLLAIW
                     RTHFHPTMRKPVREIPGYLRWPEADPGTGAPQGWTYSNLSRHSPNAFEKMVARIGRSA
                     AGAHRPLVYGTRADLWVGSHYLFDDLWHDHFVNCLDTKKTGRPLEFGAFDLFSACKFA
                     WGMRVRTENDATGKMEGLKEEMMRFLLASVLAQHGYSPRGTVLVVEHGTAAIRDDLER
                     VLYDCSDGKITVARSGMDGGGDALGIYAGRGKGNFRFKAAYESLHNLVHNEMGLLPGQ
                     TGPNRETRPEALHGLLKRNDALLCAMAALEQEAPERAKMLRFDLLEFMQFQELAMEIY
                     QRINGRVEHDLEGWGKQIVQVQRNGHPYFRRKSPTEVWDGGRKELTKLGPLAIAMILG
                     PDIGIARTIYNGVTEIQDKELSADPLIFDTHGVDRGEYQVVLNPFLPDRLFVFNTKGG
                     FVAECPRIWRVSRLDPEALQRAYGRARKIEGEMLAPLRARGAKLLTEQTENARHNAAV
                     LGAGKAPISTRKIAADAFESLPEPDQRGEEATPAEDQETLLDNI"
     gene            complement(10672..11316)
                     /locus_tag="VN496_00520"
     CDS             complement(10672..11316)
                     /locus_tag="VN496_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15452.1"
                     /translation="MSTELITAASAERIERIKTYHASCQRAGGDILAYWALTGLELLA
                     LKEETPHGEFEDLKKREFGDIPRACLGRYMQFAGDLKVKYPTVGYLVADRCLLTNGEL
                     SGEQRETVLQAVHEVADGKTVTAFFRELRMMRQPKKQQYHRPGQVDAQKQAAHEAKLA
                     RDHILTLAHDLGLAREELHLVDDATRDALFDACLEITTFVRVAKQSGGRKTKCK"
     gene            complement(11313..11639)
                     /locus_tag="VN496_00525"
     CDS             complement(11313..11639)
                     /locus_tag="VN496_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15453.1"
                     /translation="MMRGGLPAAVLARAYERGRKHISREVAKGAKSRLEARETDLSMT
                     LLESIRAEHFKCAWRFLEIGERTCAARHIRAAIIARQQIEKLEGCASSRPKQGYDVAC
                     PSIIDI"
     gene            complement(11636..11974)
                     /locus_tag="VN496_00530"
     CDS             complement(11636..11974)
                     /locus_tag="VN496_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15454.1"
                     /translation="MTAPQVIELPLFQASREDPNVQRFLRLLEAHGHLTRAKLSAITG
                     WSERQIRMLAEAAGDDVVRGQNGFCLTDRALRENLNEVQETAEMSISQGKKMIRYGFR
                     LKRRIHARVA"
     gene            complement(11971..12300)
                     /locus_tag="VN496_00535"
     CDS             complement(11971..12300)
                     /locus_tag="VN496_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15455.1"
                     /translation="MISREGSKAAKEGAIQPVANFASSRGTVARKWNGFWPEPAHIEP
                     VEENVLRPEVARRLVQFGLRERLLSFPRRFEVSEVPQILCPPRRATECAPYHERIVLF
                     GERNSLR"
BASE COUNT         2361 a         3983 c         3336 g         2699 t
ORIGIN      
        1 acggccacgt agatcgcgcg cgcgccgagg tcgatgccgg cggcgtgggg atggagggtg
       61 ggcagttggg tattggtttt cgcggctttt ggtgggcgga acgaatcaga tggaagaagg
      121 aggaaagaag cggaatatca atgccttatg aggctcaagg agcatctgga atagatttcc
      181 agtaaaaagc cggatgtttg caacggtatg acacacattg tcatggaaat tgcaccctct
      241 cagaaaggcg attgcaccgg ctcattctga gtcttggcgg agcgcttatg gcgatttgac
      301 cagccattca gagtaaacaa caactgctgc acctcgacta aattcaggtc atccagaatc
      361 cgcccatgga accgttccct cagaaaatga ttcatcactg ccgcgggaac gtgcaattgc
      421 tcgataatcc acaaaagatt gcgccgttga ttcaacacca ggctgccatc ttcaatcgct
      481 ccatccagat catccgcaag cctgagacaa cgattcttca acagggtaaa ttcgtcgcgc
      541 tcctctacct ccttggcgct ttcaatccgc cggccaatta ccgacgaaat gaactccaaa
      601 cggaactccc gatcgttgcg atcccaaccc tgcgcagcgc atgccgccgg ccaaagcgaa
      661 ccctggaaat aagcgatttg cttggagttc atttcaactc cggcaacaaa gcccgcgctt
      721 tggccgcaac cgcttgataa cgcttaaaaa aacgatcatc cacatcacat cgccgaccga
      781 ttgagcggca ggcgttcatg acagtgcccc gatcgtgttt gaagcgccgg ccagtgtaat
      841 ttttccccca tcccaggact tcaacgcaca atttcatcgc gaccatcctc gcggtgacct
      901 tgggctctga acggttttgg ctgagcatcc acgtgcgcca ccgttcacat tcacgcgcga
      961 attcaaccgc ggccaactcc atgacaactt caagcagatc ttcggcctgg tgagactgat
     1021 gcttgacccg caaataaaga cgctccaccg cagcgcgcaa agagcgcact tcatcaatca
     1081 agctccggcg atccaactcg acgcatccga cctgcagcac tggacgcgtc tccgattccg
     1141 ggaacattgg tgatttgcca ctcatcgttc ccgatcctcc ggtcgcacga actccagatc
     1201 gagcgccttg aaaatctcct cctccgtcgc gctgcccagg catttaccgt tgtcgaacac
     1261 cccaaaatgc ggattccatc ggcgcccgag cttctccgcc tgttggcaga gatagatgtt
     1321 gtgctccttg ctcccggtac ggcagaccag caaactgcca aaattcgtcg gcgtattgaa
     1381 aaacatatcc ttgaacggcc gctgcgcgat ccagacgtcg agctgcacgc cattcgccag
     1441 ccgagtgaga atggtttgcg gaccatcact gatcggctgc gttttcgcca gtacgcgcgc
     1501 gcgcaaggcg ttcacctgat ccgccagcgg caggcagacc aggtcaatat cattcacgaa
     1561 aggccgccgg cgccggatgc tcccggcgat ctcgattcgt tcacaaaacg gcgccagttc
     1621 ggccttgatc ttctcggcga gttttaatgc ctggttgaat ggtagcgcag tcatccggaa
     1681 tgtttcccgc gacggggcga aggaggcgaa ggatcaggcg gccaaccagc ctgcctcaaa
     1741 gcaactgcct cggcgtaaaa ttcgcggctg taaagaaatc cgcaggccca gacgcggatg
     1801 gcaaactctt caggcgtttc cccgctgaac ggtttgaaga aacgtgtttg agcctcagcc
     1861 tctcgcttca gggcggcaat tacctcgggc ttggtgttac cccaaaagat tttgtaatcg
     1921 ggcttcgctg ccatcgctgc ttcgcgggca attttcatac taaaacctcc aatggactgc
     1981 gcacactgag cgcttccgca tgcaaataac ccatcgttgt ctccagcgac ctgtgtccca
     2041 ttgcctcctg gatcgcccgc ggattctgcc cgctgttcaa gcaatgcgtg gcgtaagcgt
     2101 gccgcaactc atgcggcagg atcgcgagcc cgagcttgcg gccggcgcgc ttgacggcgc
     2161 gctgcacatt acattccaac atgcgccatc gaacgatccc gcctgtgcgc ggatcggcgc
     2221 acggcgccgc caggggaaac aaccaggccc agggccaggc gaattgcgaa tgcggatact
     2281 ttcgcgcgat ctgattcggc aatttgatcg gcacacccgc cgccacatca cgtcgccaca
     2341 cagcccgcgc tgattccatc tgcgctttga tctcgccagc cagcgaacac gggatcgcca
     2401 ccacgcggtc cttgcggcct ttgccctggc ggatgaccaa ccggctgtca tccagccgca
     2461 catcgcgaat acgcagattc agcggctctg aaacgcgcag cccgcagccg taaatcaact
     2521 tcaccaccag ccgtaccgga taaccgctgg cgtccctgac gccgcccagg agcgcgcgcg
     2581 tttcctggac tgagggagca ttacgaacaa cggcggcgcg tttggcgcga agcgaatcca
     2641 cgcgttgcaa cggccggccg agcgcctcgc gatagaaaaa gatcaaagca ttgaatgcct
     2701 gattttgtgt tgaagcggac acatcatcac gggcgagcgt ggtcagaaac acctcgacct
     2761 tttgctcgct ggccaggccg gagcgcagct tgcggacgaa aagcatatag cgtcgaagcc
     2821 agccgcagta cgattcctca gtggacagcg agaagtgctt gcggcggatc acctgccgca
     2881 aacgttcaat ggcatgctct cgtttcatga tatgcgaaca ggtgtcgcct aatccttgtt
     2941 gtgccacttg atcgtcacgc ctacgaacgg gtagccgtga gttttgatga accattgaag
     3001 catctccggc cagtccccga agccgtcggc tcgtgcgaac gcatcgcggt cactttcttt
     3061 ccagagccgc attgcgtcga ggtcgaatgg ctcgacggca gtgatgaccg attcacgcag
     3121 cacgcgctgt tggcttcgat acggttttcc cgtccacatc cgcaggctga ttttgtcgcc
     3181 agctttcggc atccgtttgg gagtcgggcg cacggtctgg catttcgtcc ccgcttccac
     3241 gagcggagca aattgaggtt tgaacattct gacgtggcac aacaagccgc tacacccgca
     3301 atgactgccg cgttgggcat cattggcgag ttcggagcgt ataggcgcgg cagtcatggg
     3361 gtgagcttgt cgttaagcac ttagtctggc atctttcgat ttgggtggtt ctttgccttg
     3421 tccgcgagcg cgaaaatttc cttcgcatac tcaggatcat cttgttcagc ttcgactgcg
     3481 taggccgtga gagcaggtcc ggcatacttg tctttgagcc ggataacgaa gaaatccgaa
     3541 tcctcggtgt ccccaaagtc aacggattcc gcctttacgc gcacgtctcc actttcgtcg
     3601 cggatgattc gtggtgttgc gacacgcagt ttgatgagcg cgtatttgcc acgtctcggg
     3661 ttcgtgatgt ttcggtctag tttcatattc gtgcttaacc attcgatgca ggcgacggcg
     3721 attcgcccac aggtttgttc gttgcgtcag gctcgccgcg cctgatctcg ccgttcagcc
     3781 tcataggaac gtctcctgta attcgggcgt tttcctttcg gccatgcaga ttgtgtcgtt
     3841 gtggtcgctg ccgtggcaca cgagcagcac ttcttcgatg ttccagcctt tgcccatgcc
     3901 gaccgtgttc cagccgaagt gcagcaccac accaccgacc ggcacgaggc gattgatttg
     3961 cctcttgagt cgtttgcgcg tcatgccgag cagcgcatcc gtttgcttca tgggcagacc
     4021 gatggagtca tagcattcct tcacctggcg cggcgagtat ggcgggtcac agattaccag
     4081 gtcagccacg atgtctttgt ccgcgagcat cgtcaggaag tcctcggcgt ccatgtgata
     4141 ttccgccgcc gtgttcggat tcaggtcatt ggtgtaggtc gtccatcgct tgttccgcga
     4201 gaagcagtcc accgagattt tggatttcat cagatacttt ttcacgaagc ccgcgatgga
     4261 tggcacgtcg aaagtgtcag agttcggcat gtcccacacc ctcgacaatg aggctgaaca
     4321 accgcatgga gctaacccgg cgggcttctg cagttcgatg gccagagtgt tattcatgcg
     4381 tcttggttct tcgcgccggg tagctcatgc ggagcgttcc gcgaatacat gtcttcaagg
     4441 atccagacgt tcaatgtcca gcaggcccac attagaccta cgacgatttg gccatatccc
     4501 agcgaggcac cgaagaggaa aatgatgaac aggaagtatc tcgctataac aagccgtctg
     4561 aaggttgatt cgcggaacaa ggtcgctgga ggcgacgctt gttccgccgc agtcgattcg
     4621 ggaacctttg gattggagtc agttttcatc agcgcgcctc atctcccgcg ttctgccgca
     4681 tttgcttttc gagagcttta accattcggc gagctttcga gcatttgagc aggtgttctt
     4741 tctgtgcgtt ttgctccatt acatgagcgt atcggaatcc cgcgccgttt ccggggtcgc
     4801 atcgcgtcag gactttcttg cacccattgc agatcagtgc gcgccgattt ccatagccgt
     4861 ccaccgatga cggaagcttt ttgaatttga acgcttgcgg cagaacaatc cggttggagc
     4921 gaactgccga cccgcgttgc aattgagttt ttggcataga gttacgttgt tggttttccg
     4981 ggcagtcgct cacccggagc gttccgcgcg agcgctcgct cccccgctgc ggggggagcg
     5041 tctggcggca taggaagtgt cgcccagtgc gttgggttta attcgattgt gtcgctacgg
     5101 ttctcgtaaa ttatccatgc gccccgtccc acccatatac atgtggacca gtaaccgatt
     5161 tccatttgcc catcgctcca caataacagt cttgtcccat cttggggcgc agtctcaatc
     5221 ggatgccatg ccgcccggcc gctttctcgc gcggaacgcg acgaggtatc agcttcgttg
     5281 tcccttgctt cgctcgcgct cgctgcggaa caaactcgct gaagcgatcc cgcctgcggc
     5341 ggctgcgctt cgcttgccac ttcacttatg agcacacacc ctagaggctg gctacttggt
     5401 ttggcctgtg cgtgtccagc gtcgtccggc atcaggctgt caggtttttg tgtgctcata
     5461 agttcgtggt tcgcttacct cgtcgcgttc ggcgtgcctt gacaccactc gctgggtttc
     5521 gtgcattgga agcggcggta tttttgcaca taggtcatct ccgactccag gtgcattcgc
     5581 cagcacaaga gacagcgacg gcggcggttg ggtggcgcag gcgtgagacg gaagatttca
     5641 tctttcagcc gggtcagttc acccgcgatt ttgtgatagc gttttgcgtt caagccgcct
     5701 cctttacgcc cggctcgtcc acggaatcct tgagcagcgc gttcacgatc ttctccacgt
     5761 cgccatcagt cggcttgatt accacctgat cgccggcatc cacgatcgcg cagccgatct
     5821 tcttcagctc tgcggcggac aattttgcca atgcttcctt gtccggcttt tcttccctgc
     5881 gaatcagcgc atcgggatcg tcgaaatgtt tgcggatcaa ctccaccacg cggtcggcat
     5941 tatcgaactc gattccgcct ttgcccttct gaaagccgat cttcaccccg tgcagaatca
     6001 atgtgcgcgg cttgacgaac aactcgggcg tttcctcgat cgctgccttg agacgcgcgt
     6061 gccgttcagc cgtgcccgca acgaagcgtt tcaacgccgc catgtgccgg cgcttgaccg
     6121 ctgccaattc cgattcaata tcgcccacca attgcgtcag gcgctcgcga gcgtcggcga
     6181 agtctttggt gaaccgttcg atctctgcta atgatgattt cattttgcac cctttccgat
     6241 caatcgcgcg gcatccttac atgtctcggc tcgcaacgca tgttcgcgcg ccagccgcac
     6301 attttccgca tgcttcttgt catcgcctgg gacgtaattc aaggcactga gagccgcctc
     6361 cgcggccagc gtcctgaatc tggcggcttc ggccagtaat atggattcgt ttttcacttc
     6421 gccccttcgc gggataaagc atcgcacgcg gggtcctcga cggctgcccc gcggcaagca
     6481 gggccttctg gtttaggatt tacatcgagg ctgtctccgc atgcgaaaat ttcagcagcg
     6541 ccggcggggt aacgcgctcc ggcagcacct tggggccgat ggggaaccgg acttgaagaa
     6601 tccggagagc ttccgacgct gctaaaaata attccttcgc ttacttcgga ccttcgcggg
     6661 agaccccatc ccttcaggtc cgccgcgcac gtcgggcaaa tgcaattcgt gaccggcgac
     6721 cagggcagga aactgaaccg cgcagtgtgc cgcgtgtgcc cgcaccagga acacacgatg
     6781 atcttcgtgt gccggaacca gaacgcgcgc acgcgcgatc tcgcgaagcc aaaccattcg
     6841 cgaaggtgca gccaggcaaa gacgatcctg gccaaaacat ttcccgcgaa gaggcgaagg
     6901 cggcgaaggg aagtttcacc gcggagacgc ggaggcgcag agactgcgcg atgcaacgcg
     6961 agcgcctccc aaataatcca gccaaacaag gcgcagtaaa accagagaat gatcttcatc
     7021 gtccgcctcc tttcttctcc ttcagcaata aactccggac aaaattctct acagattcgg
     7081 cccgactcag cgtccgtcta tagaaagact cggcatcgac gcgcagaaac gcggccagca
     7141 tcaacatgac gttttcggtg tcggtcagac acgcggtgca aatatccgtg tccaatgccc
     7201 aactgcatcc ttccaggcaa gcgcggtttt ccgtgcatcc acatcttcgg catttccgct
     7261 tcgccacctt cgccccttcg cgggaaatct tcatgctcat cgcccgcctc ctttctccag
     7321 ttgcctgatc cggcgtgcga tgatcgtggc cttggtcttc tggccttgat aaacgcaaag
     7381 ttcgtgcgcc ttgcgcagat cattcaggtc cgccgccgtc cagtgccaca aacgtgagtg
     7441 cgcatcatcc acgcttgtat gaaccagccc gagcacctgc tgcggaattc cgtaattgtc
     7501 cggcttcgcc cccttcgccc cttcgcggga aatcttcttt gtgctcattg accctcctgt
     7561 ttttgtttca gcttcgtggc gatgcccacg atctccacga aatgtttcca gccaaaccgg
     7621 tcgttgcgtt tgttggcgag ctgcgcgccc cgcgcgagga accgcgtgta tttcccgaga
     7681 cccatctcct tgccgatcca tttcaccagc tcggccgcct cgcctcccgg cgccggcagc
     7741 ttgtaaaaat ccgcgatcgc cgccagatcc ttctccttcg gcgcatcctc gagctgcagc
     7801 tcccagatgc cccgcttgcg aagctgcttg agcgactgcg agaattcccc gcgctccagc
     7861 tcatgccgga acacattcgt gccgcacaaa accaggccgc actggctgat ctcctgcaac
     7921 tgccgcaaca cgctcaggca cttcaccagc gaagacttct gataactcac gaaacattca
     7981 tgaatctcgt cgatcgccag cagcttcgat ccatccagat aattgccgac ccgttcgcga
     8041 agctgctcga aactggtgcg cacgctgata tggcacgcct cagcgatcgc tttcatcatc
     8101 gcctgcacgc cggcgctcgc gggcatcagc acgtaaaccg tgttcccgtg attgttccgg
     8161 cgcgcgacct ccttcaaact ctcggtcttg ccgatctgcg actcgccgta aatcatcgcg
     8221 atcgtttgca tgatcagcgc ctcgcgacac accttctcga tccggcggaa cacgctcgtc
     8281 tcgatgaacg gcagccggcg tgccagcgcc cgttcctcgg ccagctcctt gaatcgcgcg
     8341 atccgttcgc agagggaggt caggtccacc agctcgcccg tatccgtgtt ccggtatttg
     8401 ccggtccaca cgcgatacat cgtcgtcgtg ctgatcttcg tttcgcgcgc cagatcctcc
     8461 cagctccatt ccatctcctt cgcgaacgcg aacagccagc gcaccaggtt gcgatgctcc
     8521 tccggataat cggcggtctt caccgtgcag gtgtccccgc tcacattgat gacgccggcg
     8581 ccgccctttt cgatgaccgc aagtttttga tcgtcctcca ttttgattac tctcaaatgt
     8641 tgtccaacaa cgtttcctga tcctccgctg gcgtcgcctc ctcgccgcgt tgatcaggct
     8701 ccggcagact ctcgaaagca tccgccgcaa ttttgcgcgt cgagatcggc gctttgccgg
     8761 cgccgagcac tgccgcgttg tgccgggcgt tctctgtctg ttcggtcagc agcttcgctc
     8821 cgcgcgcgcg cagcggcgcg agcatttcgc cctcgatctt tcgcgcccgg ccgtaagcgc
     8881 gctgcaacgc ttccggatcg agccggctca cgcgccagat gcgcgggcac tccgccacga
     8941 acccgccttt cgtgttgaac acgaacagcc ggtccggcag gaacggattc agcaccacct
     9001 ggtattcgcc gcggtcaacg ccgtgcgtgt cgaagatcaa tggatcggcc gacaactcct
     9061 tgtcctgaat ttcggtcacg ccgttgtaga tcgtccgcgc gatgccgatg tccggtccga
     9121 gaatcatcgc gatcgccaga gggccgagtt tcgtcaactc cttgcggccg ccgtcccaga
     9181 cttcggtcgg tgatttgcgg cggaaatatg gatgcccatt gcgctgcact tgtacgatct
     9241 gttttcccca cccttccaga tcgtgctcca cacggccgtt aatccgctga taaatctcca
     9301 tggccagctc ctggaactgc atgaactcca gcagatcgaa tcgcagcatc ttcgcgcgct
     9361 cgggcgcttc ctgctccagc gcggccatcg cgcacagcaa cgcgtcattg cgcttcaaca
     9421 aaccgtgcag cgcctccggc cgcgtctcac ggttcggccc ggtctggccc ggcagcaacc
     9481 ccatttcgtt gtggaccaga ttgtgaagcg attcgtaagc cgccttgaac cggaagttgc
     9541 ccttgccgcg cccggcgtaa attcccagcg cgtcgccgcc gccatccatt ccgctgcgtg
     9601 ccaccgtgat cttcccatcg ctgcaatcgt aaagcacgcg ctccaggtcg tcgcgaatcg
     9661 ccgccgtgcc gtgctcgacc accagcaccg ttccacgtgg agaataaccg tgctgcgcca
     9721 gcacgctcgc cagcaggaag cgcatcatct cctccttcag gccttccatt ttcccggtcg
     9781 cgtcgttttc ggtccgcacg cgcatgcccc aggcgaactt gcaggcgctg aacagatcga
     9841 acgcgccaaa ctccagcgga cgcccggtct tcttcgtgtc caggcaattc acgaaatggt
     9901 catgccacaa atcgtcgaag agataatgcg accccaccca cagatccgcg cgcgtgccat
     9961 acaccagcgg ccgatgcgcc cccgccgcgc tgcgcccgat gcgcgccacc attttctcga
    10021 aagcgttcgg cgaatgccgc gacagatttg agtaggtcca gccctgcggc gcacccgtgc
    10081 ccggatcagc ttccggccag cgcagataac ccggaatctc gcggactggt ttgcgcatgg
    10141 tcggatgaaa atgcgtccgc cagatcgcca gcaacacgcg atgcgccgcc ttgtcgccgc
    10201 cgcgccgctt gttctcctcg caaagtgatt tccagaattc ccggaacgcc cgaggcagcg
    10261 cggcgtggca cgccgaacca gtcagtttga tgcccgcctt cgcgtgatcc agcagcaccc
    10321 gccagtcgcc ggattttcga taagcgaaat aaagggtata aaaccgctgt gcagagcctc
    10381 cgcgcactcc ccgcaaccgc gctgcataga acctgcaggt ggcacgcttg ttgccggacg
    10441 cggcgatgga ctgcatcacc ttcagccggc tgagcacttg ggcgcgggcc ggctccggca
    10501 gcgcgatgaa ctcagcctgc gcgctggccg ggatggaaaa cgccgccggt gtttcatgcg
    10561 cgacacaaac ggggtccgtc gaaacccccg gacccgagga aaggtccgag cgcgctccgg
    10621 agttctcctc cggcagcatg ccggcggcga gaggttctat tggatcagga ttcatttgca
    10681 tttggttttt ctgcctcctg attgtttggc tacccgaaca aaagtcgtga tctccaggca
    10741 cgcatcgaag agcgcgtccc gcgtcgcatc atccaccagg tgcagttcct cgcgcgccag
    10801 cccgaggtca tgcgccagcg tcaaaatgtg gtcgcgcgcc agtttcgctt cgtgcgcggc
    10861 ctgcttctgc gcatccactt ggcctggccg gtgatactgc tgcttcttcg gctgccgcat
    10921 cattcgcaac tcacggaaaa acgccgtcac ggtcttgcca tcagctacct cgtgaaccgc
    10981 ctgcaaaacg gtctcgcgct gctccccgga aagctcgcca ttcgtcagca aacagcggtc
    11041 agccaccaaa tatcccacag tgggatattt gactttgaga tcgccggcga actgcatata
    11101 acgccccagg caggcccgcg ggatgtcgcc gaactcccgc ttcttcagat cttcgaattc
    11161 accgtggggg gtctcctcct tcaaagccag aagctccagt cccgtcagcg cccaatacgc
    11221 gagaatgtct ccgcctgccc gctggcaact ggcatggtaa gttttgatcc gctcaatgcg
    11281 ttcagccgaa gccgcggtaa tcagctcagt gctcatatgt cgatgatgga tgggcacgct
    11341 acgtcatatc cctgtttggg ccgcgatgaa gcgcagccct ccagcttctc aatctgttgc
    11401 cgggcgatga tcgcggcccg gatatgccgg gcggcgcagg tgcgctcacc gatctccagg
    11461 aatcgccagg cgcacttaaa atgctcggcg cggatacttt ccagcagcgt catggataga
    11521 tccgtttccc gcgcctccag tctggacttc gcccccttcg ccacttcgcg ggaaatgtgt
    11581 ttccgcccgc gctcatacgc gcgcgccaga actgccgccg gaagcccgcc cctcatcatg
    11641 ccaccctcgc atggatgcgc cgcttcagcc tgaagccgta gcggatcatt ttctttccct
    11701 ggctgatgct catttccgcc gtctcctgga cctcgttcaa attttcgcgc agcgcccgat
    11761 ctgtcaggca gaatccgttc tggccgcgca cgacgtcatc gccggcggcc tcggccagca
    11821 tccggatctg ccgctcgctc cagcccgtga tcgccgagag cttcgcccgc gtcagatgcc
    11881 cgtgcgcctc cagcagccgc aggaaccgtt gcacgttcgg gtcctcgcgc gacgcctgaa
    11941 acaacggcag ctcgatcacc tgcggcgcgg tcatcgcaat gaattccttt cgccgaacaa
    12001 cacgattcgt tcatggtagg gcgcgcactc cgttgcgcgc cgcggcgggc agaggatctg
    12061 gggaacttcg ctgacttcga atcttcgcgg gaaactcaga agccgttccc tcaatccgaa
    12121 ctgcaccagc cgccgggcga cttccggccg cagcacattt tcctcgactg gctcgatgtg
    12181 cgccggctcg ggccagaagc cgttccattt ccgggctact gttccccgcg aagaagcgaa
    12241 gttcgcgacg ggctgaattg cgccttcctt cgccgccttc gagccttcgc gggaaatcat
    12301 agttttctcc ttttcaaaaa accggcggcg ctggtccggg taagctgcgc tgatccgccc
    12361 ggaccgggcc gccagttgg
//
LOCUS       DATMZQ010000014         5033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1737517, whole genome shotgun sequence.
ACCESSION   DATMZQ010000014 DATMZQ010000000
VERSION     DATMZQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737517"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VN496_00540"
     CDS             <1..632
                     /locus_tag="VN496_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXF15456.1"
                     /translation="LDASSVSRFTQQLSLGLSILLIAAYALGMLFSLKTHREFFASAN
                     VETGSETPWPMGLALTTLAGITVVVALVSEIFVESVQEAALKLGMTQAFVGFIVVGLV
                     GGAAEMASAISGARKNRLDLSVGIALGSASQIALFVAPVLVLLSYFIGPAPMDLQFWP
                     GAVVMVLIASITAALVTNSGRSAWFVGFLVLLVYVIFAMTLYLLPPKTQ"
     gene            629..1309
                     /locus_tag="VN496_00545"
     CDS             629..1309
                     /locus_tag="VN496_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXF15457.1"
                     /translation="MTTSAQTTDDAGTARVGVLDPIQRVSEVLFGLIMVLTFTGSLSV
                     AEAGRDDVRAMLIGALGCNTAWGLIDAVLYLMGCLAEKGRNLIAFREVRKASDTQYAQ
                     HLIAEALPPVVASVLKPQELELIRERLSHLPEPPRHARLSKEEWLGAIGVFLLVFLST
                     FPLVIPFMVMSNAAPALRVSNAIAIVLLFIAGYAYGRITGRHPLVIGIAMVILGLALV
                     SMAMALGG"
     gene            1446..2114
                     /locus_tag="VN496_00550"
     CDS             1446..2114
                     /locus_tag="VN496_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXF15458.1"
                     /translation="MRRISKLLPAIALAALVGGIAFPSAAQGVGHREGTWEFQLPLTY
                     TESWTANGDGGSKADVNAGFGLGFGFAYNFNDHLQLGGSFNWSSRSYDATLVQTNGVP
                     RHASGTLESSTIALNGVYFLFAGPLTPYASASIGSTFVDSNIPNGQSSTACWWDPWWG
                     YICNDYTPTKTETDVSYNVGLGVRWDISRVMALQGGYNRMWLDSSHSTPEFNNWTLSF
                     IFRM"
     gene            2223..2783
                     /locus_tag="VN496_00555"
     CDS             2223..2783
                     /locus_tag="VN496_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15459.1"
                     /translation="MKGVLSVAALLLAVAAAPSLHAEKAGVVVADSVEAIVTVVSVDR
                     EARVVTIRGPEGRTAIINVPKEAQNLDQVKPGSKFKVTYLSSVALSLRKGTGTPSASH
                     NEKVQLAKKGETPGGTAVRVREINAVVEAMDPATRGLTLKGPEGGTVELKVREDVKAY
                     SEVKVGDTITVSYTEGLALKMLPPQQ"
     gene            complement(2894..3676)
                     /gene="fabI"
                     /locus_tag="VN496_00560"
     CDS             complement(2894..3676)
                     /gene="fabI"
                     /locus_tag="VN496_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HXF15460.1"
                     /translation="MTLEVPRPVLKGAKALVVGIANQDSIAYGCAKAFRELGADLAIT
                     YVNEKTRTYVEPLAKELEAKIFMPLDVATPGMLEAVFERISREWGEIDIVVHSIAWAP
                     KADLQGGLLNCSANGFMQAMDISCHSFVRMARLAEPLMKNGGTLFAMSYYGANKVVAN
                     YNVMGPVKAALEASCRYLAYELGGKGIRVHAISPGPLKTRAASGLKDFELLLNEAAQR
                     SPLGELVDTMDVGYTCAYLATPYARRLSGETLYVDGGVNIMA"
     gene            complement(3748..4950)
                     /locus_tag="VN496_00565"
     CDS             complement(3748..4950)
                     /locus_tag="VN496_00565"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HXF15461.1"
                     /translation="MADAILVLNAGSSSIKFSLFADRNPQPDLLLRGQIEGLYTAPVF
                     AAKDAAGANIGARKWEKGTRLGHDGAITYLIEFLRGHREDHHLVAVGHRVVHGGLEYS
                     QPTLVSAQVLARLEHFIPLAPLHQPHNLAPIRVVAERMPQLPQVACFDTAFHRSQPEL
                     AQAFALPASITDRGVRRYGFHGLSYEYIASALPDLDEKANNGCVIVAHLGNGASMCAI
                     KAGRSVASTMGFTAVDGLPMGTRCGSIDPGVMLYLMDELKMDTRAIEKLIYQQSGLLG
                     VSGISSDMRTLLGSDQPRARLAVDLFVYRIGRELGSLAAALGGIDVLVFTGGIGEHAP
                     AIRERVCREAAWLGVELDAKANATRAPRISTQASRVSAWAIPTNEELMIARHTRSLLA
                     AAASKVTA"
BASE COUNT         1018 a         1459 c         1519 g         1037 t
ORIGIN      
        1 agttggacgc cagctccgtc agccgattca ctcagcaatt gagccttggc ctgtccatcc
       61 tgttgattgc agcctacgcg ctcggcatgc tgttttcgct caagacgcac cgggagtttt
      121 ttgccagcgc aaatgttgag accggcagcg agacgccctg gccgatgggt cttgcgctga
      181 ccacattggc tggcataacg gtcgtggtag cactggtcag cgaaatcttt gtggaatcgg
      241 tgcaggaggc ggcattgaag ctcgggatga cgcaggcctt cgtcggcttc atcgtcgtcg
      301 gcctcgtcgg tggtgcagcg gaaatggctt cggcaatctc gggggcacgc aagaacagac
      361 tggacttgag tgtcggtatt gcactgggga gcgcgtccca gatcgcgttg tttgtcgcgc
      421 cggtgctggt gctgttaagc tatttcatcg gccccgcgcc aatggatctg caattctggc
      481 cgggcgcggt agtgatggta ctcattgcga gcatcacggc agcgctggtg accaacagcg
      541 gtcgatctgc atggttcgtc ggttttctgg tgttgctggt gtatgttatt tttgccatga
      601 ccctttacct gttgccaccc aagacacaat gacaacctcc gcgcaaacga ctgacgatgc
      661 tggtacggcg cgcgtaggcg tcctggatcc catccaacgc gtgtccgagg tgctattcgg
      721 attgatcatg gtgctcacgt tcaccggttc gctaagcgtg gcggaggcgg ggcgcgacga
      781 tgtgcgcgcg atgctgatcg gtgcactcgg ctgtaacacg gcctggggcc tgatcgatgc
      841 cgtgctgtac ctgatgggct gtctcgctga aaagggccgc aacctgattg cgtttcggga
      901 agtccgcaaa gcttctgata cgcagtacgc acaacacctt atcgcagaag cgttacctcc
      961 tgtggtggca tccgtgctaa agccgcagga actcgagctt atccgcgagc ggctatcgca
     1021 tttgccggaa ccgccgcgtc acgcgcgcct gagcaaggag gagtggctgg gcgcaatcgg
     1081 cgtattcctg ctcgtgttcc tttccacgtt tccgctcgtc attccgttta tggtcatgag
     1141 caacgcggcg cctgcattgc gcgtctccaa tgccatcgct atcgtgctgt tgttcattgc
     1201 cggttatgca tacggacgca taacgggccg ccacccattg gtgatcggta ttgcaatggt
     1261 cattttagga ctggctctgg tcagcatggc aatggcactc ggcggctgaa aagatgcgtc
     1321 gttgccagga tgtcgtcagg ggccgacatc gacagtgatt gacctgaatc aactacaatc
     1381 gcactcagat gacatttaat cagttttttt ccgaaatagg tcacaactaa aagaggggaa
     1441 cgaatatgcg acgcatctca aaattacttc ctgcaatcgc gctggcggcg ctcgtgggcg
     1501 gcatagcatt cccatccgcg gcacaaggtg tcggacaccg cgagggcacc tgggaatttc
     1561 agttgccgct gacttatacc gaatcctgga ccgctaacgg agatggcggc tccaaggcgg
     1621 acgtcaacgc cggattcggc ttgggatttg gcttcgccta caacttcaac gatcatttgc
     1681 agcttggcgg tagtttcaac tggagcagcc gcagctacga tgccaccctc gtgcaaacca
     1741 atggcgtgcc tcggcatgcc tcgggcacgc tggagtcttc caccatagcg ctgaacgggg
     1801 tttactttct gtttgccgga ccgctgacac cctacgcatc agccagcatt ggcagcacgt
     1861 tcgtggactc caatatcccg aacggacaat cgtcgactgc atgctggtgg gatccgtggt
     1921 ggggatatat ctgcaacgat tacaccccga ccaagaccga aacggacgtc agctacaatg
     1981 tgggactggg cgtgcgttgg gacatatcgc gcgttatggc gcttcagggc ggctataacc
     2041 ggatgtggct cgactccagc cattcaacgc ccgaattcaa caactggacg ctgagtttca
     2101 ttttccgcat gtgaccccgt gggacactgt cacggtgtcc caatccctgc aagattagaa
     2161 gtcggaaatc cggtgcccca gtcggggcac cgggcagttt gcttaaccat aggagatgtg
     2221 aaatgaaagg tgttttatca gtcgccgccc tgcttctggc cgttgccgca gcgccatcgc
     2281 ttcatgccga aaaggcgggc gtggtcgttg ctgactcggt ggaggccatc gtaacggtgg
     2341 tatcggtgga tcgtgaagcg cgcgttgtga ctatccgtgg tccggaaggg cgcacggcga
     2401 tcatcaatgt gccaaaagaa gcgcagaacc tcgatcaggt caaaccgggc tcgaaattca
     2461 aggtgacgta cttaagttcc gttgcccttt cgttgcgcaa gggaacgggc actcccagcg
     2521 caagccacaa tgaaaaagtg cagctcgcca aaaaaggcga gaccccgggc gggacggcgg
     2581 tgcgcgtgcg cgaaatcaat gccgttgtcg aagccatgga tcccgccacg aggggcctga
     2641 cgttgaaagg accggagggc ggtacggttg agctcaaggt gcgcgaagac gttaaagcct
     2701 atagcgaagt caaggtaggc gacacgatca ccgtgtcata caccgaaggc ctggcactga
     2761 agatgctgcc accgcagcag taacggttag aagttggtac ggatgcgcgc cagcacgagg
     2821 gcgcgcagtc cggttccgcg gatcgtgttt caagacgctt tatcgtgtgc caaacaggat
     2881 actgttcgca cgactaagcc atgatattga cgccaccatc cacgtacagc gtctccccgg
     2941 acaaacgccg cgcgtacggg gtcgcaagat aagcgcaggt gtagccgaca tccatggtat
     3001 cgacaagctc gccgagcggc gagcgctgcg cggcctcatt gagcagcaac tcaaaatcct
     3061 tgagaccgga ggcggcacgc gtcttcagcg gaccgggcga gatcgcatgc acgcgaatgc
     3121 ccttgccacc aagttcgtag gcaagataac ggcacgatgc ttcgagcgcg gctttcaccg
     3181 gtcccattac gttgtagttg gccacgactt tgttcgcgcc gtaatagctc atcgcaaaca
     3241 atgttccacc gtttttcatc agcggctcgg cgagccgcgc catgcgcacg aaggaatggc
     3301 aggagatatc catcgcctgc atgaagccgt tggcggaaca gttgagcaac ccgccctgga
     3361 gatcggcttt cggcgcccag gcgatcgagt gcacgacgat gtcgatctcg ccccattctc
     3421 tcgaaatcct ctcgaacacc gcttccagca tacccggcgt ggcaacgtcg agcggcatga
     3481 agattttcgc ctccagctct ttcgcaagcg gctcgacata ggtcctggtc ttctcgttga
     3541 catacgtaat cgcgagatcg gcgccgagtt cgcgaaacgc tttggcacag ccgtaggcaa
     3601 ttgaatcctg gttggcgatg cctacaacca gcgctttggc tcctttgaga acgggacgcg
     3661 ggacttcgag tgtcattttc tctcctaaat atgcatccgc tgtggtgttt gacatcgcgc
     3721 gccccggaac acggccgcgc agcaatgtca ggcagttact ttcgatgccg cggcagccaa
     3781 cagcgagcgt gtgtggcgcg cgatcatcaa ttcttcgttg gtcggaatgg cccatgccga
     3841 gacacggctc gcctgcgtgc tgatgcgcgg cgcgcgcgtt gcattggcct tggcatcgag
     3901 ttccacgccg agccatgctg cctcacgaca aacgcgctcg cggatcgccg gcgcgtgctc
     3961 gccaatgccg cctgtgaaca ccaggacatc gatgccgccg agcgctgctg cgagagaacc
     4021 aagttcgcgg ccgatacgat agacaaacag atcgaccgcg agcctggcgc gaggttggtc
     4081 gctgcccagc agcgtgcgca tgtcgctgga gatgcccgaa acaccgagca gcccggactg
     4141 ctggtagatc agtttttcga tggcacgcgt gtccatcttc aattcgtcca tcaggtacag
     4201 catgacgccc ggatcgatcg agccgcaacg ggtgcccatg ggcaggccat ccacggcggt
     4261 gaatcccatt gtgctcgcca cgctgcgacc tgccttgatc gcgcacatgc tggccccgtt
     4321 gcccagatgc gcaacgatga cgcatccatt gttcgctttt tcatccagat ccggcaatgc
     4381 gctcgcaatg tactcatagg acaggccgtg aaaaccataa cggcggacgc ctcggtcggt
     4441 aatggacgcc ggcaacgcaa aggcctgcgc cagctccggt tgcgagcgat gaaacgcagt
     4501 gtcgaagcac gcgacctgcg gcaactgtgg catgcgttcg gcaacgacgc ggatcggcgc
     4561 caggttgtgc ggctgatgca gcggcgcgag cgggatgaag tgctcgagcc gggcgaggac
     4621 ttgcgcgctg accaatgtcg gttgcgaata ttcgaggccg ccatgcacaa cccggtgacc
     4681 cactgcaacg aggtggtggt cttcgcgatg tccccgcagg aattcaatca ggtaggtgat
     4741 agcaccgtcg tgacccaggc gcgtgccctt ctcccacttg cgcgcgccaa tgtttgcacc
     4801 agctgcgtct ttggcggcaa agaccggggc ggtgtaaagg ccttcaatct ggccacgcag
     4861 cagcagatct ggctgcggat tgcgatcagc gaacagcgaa aatttgatgc tcgacgagcc
     4921 ggcattcagt acgagaatcg cgtcagccat atgctcatcc cagcgcgctg gcagcacttt
     4981 cgcgctgggc tttcgccgcg agcgcggcga ccgcgcagga ggcaagccgg gtc
//
LOCUS       DATMZQ010000015         3180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1737588, whole genome shotgun sequence.
ACCESSION   DATMZQ010000015 DATMZQ010000000
VERSION     DATMZQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737588"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="mutY"
                     /locus_tag="VN496_00570"
     CDS             complement(<1..910)
                     /gene="mutY"
                     /locus_tag="VN496_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009972864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXF15462.1"
                     /translation="MEQFAERVITWQRTSGRHDLPWQRTRDPYLIWLSEIMLQQTQVA
                     AVIRYYARFLEHFPTLDSLAAAPEDDVLALWSGLGYYARARNLHGAARLIVQRHAGVF
                     PGNFADIVALPGIGRSTAGAIAVFAFGKREPILDGNVKRVLARVFGIAGFPGDKEISM
                     RMWKHAEDVLPSNDVETYTQGLMDLGAEVCIRRRPRCECCPLSGVCVAKRDNRIATLP
                     APRPKKTLPEKSTVMLILQREGQVLLEKRPAPGIWGGLWSFPEMADLVDVVGVVHRRF
                     GAQATSEGILAAVRHGFTHYALTITPA"
     gene            complement(931..2886)
                     /locus_tag="VN496_00575"
     CDS             complement(931..2886)
                     /locus_tag="VN496_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXF15463.1"
                     /translation="MKALKWVLIAAGAVVLALAAALAFIAATFDPNQYKTQAATFVKD
                     KTQRTLTIEGDIRLMLFPKLGIHLGKTRLSEVRGETVFANLDDMRISLALIPLLSRQI
                     VIDEVRLAGLRANLVKQKDGRTNFDDLLGGDKKSDGKSNQASGKPAEPAKAPMPVKLE
                     IEGVTISSANLTWKDQMTGATYEISDFDLKTGRVAPGIPTRFSLGAAVRANEPKLDLK
                     IGVEGLLTAELERQLFSVAELSVKVNGQAAGRTGLAAQLSGSAGADLQKHSANADVAL
                     KIMDSNIKAKLAVEDFAAMRSTFDITIDKLNVDQFMPAKKAGETAGTPAGPQPEQPID
                     FSPIKKLDVKGSVRIGELIAQNIKAQNVRLELMAKHGRLDLEPLTADLYQGSVKGMAS
                     IDANANRIVIKQTLAGISIGPLLRDATQHDLLDGRGSVALDVTTTGTLVTALKKALNG
                     TARIELKDGAIKGIDVAQSLRKAKALLVGGTSESDQAAATGGKTDFSELTASFIIKNG
                     VAHNSDLLAKSPFLRLTGEGDINIPDSSLNYLVKASVVASSAGQGGQELAELQGLTVP
                     VRASGPFTALKYKVEVGSVLGDSAKQKLNEKKEQMKKNLEDQLKSKLLRKPGESPSDG
                     GEPAAGHAAPPPAKPEDKLKEKLKKLF"
     gene            complement(3036..3109)
                     /locus_tag="VN496_00580"
     tRNA            complement(3036..3109)
                     /locus_tag="VN496_00580"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3075..3077),aa:Gly,seq:ccc)
BASE COUNT          623 a          954 c          873 g          730 t
ORIGIN      
        1 gtgccggagt aatcgtgagc gcgtaatggg tgaagccgtg gcgcacagcg gccaagatac
       61 cttccgaagt tgcttgcgcc ccgaagcgcc tgtgcaccac cccaaccaca tccaccaagt
      121 ccgccatctc cggaaagctc cacaagcctc cccagatccc gggcgccggc cgtttttcca
      181 gcaatacttg tccttcacgc tgcagaatca acatcacggt agatttttcc ggcaatgttt
      241 ttttcggccg tggggccggc aacgtcgcga tgcgattgtc gcgcttcgcc acgcacacgc
      301 cgctcagcgg acagcactca cagcgtggcc ggcggcggat acaaacttcg gcgccgagat
      361 ccatcaagcc ctgcgtataa gtttccacat cgtttgatgg cagcacgtct tctgcgtgct
      421 tccacatccg catactgatt tctttatccc ctgggaatcc tgcaatgccg aatacccggg
      481 ccaacacccg cttcacattg ccgtccagaa tcggttcacg ttttccgaaa gcaaacacgg
      541 cgatggcgcc tgcggtcgaa cgcccaatgc ccggcagtgc aacgatatct gcaaagttgc
      601 ccggaaagac acccgcgtgc ctttgcacaa tcaagcgggc cgcgccatgc agattgcgtg
      661 cacgggcgta gtaacccagc ccgctccaca gcgcaaggac atcgtcttca ggggccgcag
      721 ccaggctgtc gagcgtcgga aagtgctcca ggaagcgggc gtaatagcga attaccgcgg
      781 ccacctgtgt ttgttgcaac atgatctctg acagccagat caggtaaggg tcgcgcgtgc
      841 gttgccaggg aagatcgtga cgacctgaag tgcgttgcca cgtgattacg cgctcggcga
      901 actgctccat tgcgataccg gatactggac ttagaaaagt tttttgagtt tttccttcag
      961 cttgtcctcg ggctttgccg gaggtggcgc cgcatggccc gcggccggct cgccgccgtc
     1021 tgagggtgat tccccaggct tgcggagcag cttggattta agctgatctt cgagattctt
     1081 cttcatctgt tccttcttct cgttcagttt ttgcttggct gaatcgccca atactgaacc
     1141 gacttcaact ttgtatttga gcgccgtgaa tggacccgat gcacgtacgg gaacggtcaa
     1201 cccctgaagc tccgccaatt cctggccgcc ctgccccgca ctactggcca caaccgaagc
     1261 cttgaccaga tagttcaggc tgctgtctgg aatgttgatg tcgccttcgc cagtgagccg
     1321 caggaacggc gatttggcga gcagatcact gttatgtgcg acgccgttct tgatgatgaa
     1381 gctcgcagtc agttcggaaa aatcggtctt cccgcccgtt gctgcggcct ggtccgattc
     1441 cgacgttcca ccaaccagca gggccttggc tttgcgtagc gactgcgcaa catcaattcc
     1501 tttgattgcg ccatctttca attcgatccg ggcggtgccg ttgagcgcct tcttgagtgc
     1561 agtaacaagg gtgccggtgg tcgtcacatc gagcgcaacg ctgccgcggc cgtccaacag
     1621 atcgtgctgg gtcgcatcgc gcagtaacgg accgatggag attcccgcca acgtctgctt
     1681 gatcacgatg cggttggcat tcgcgtcgat ggatgccatg cccttgacac tgccttggta
     1741 caaatcagcc gtcagtggct caaggtcgag acggccgtgc ttggccataa gctcaaggcg
     1801 aacattctgg gctttgatgt tctgcgcaat tagttcacca atgcgcacgc tgccttttac
     1861 atccagtttc ttgattggag agaagtcgat gggctgctcg ggttgcgggc cggccggggt
     1921 accagctgtc tcgccggctt ttttggccgg cataaactga tcgacattca atttgtcgat
     1981 cgtgatatca aaagtactgc gcatagctgc gaaatcttcg acggcaagct tggccttgat
     2041 gttgctatcc atgattttta gcgccacgtc agcgttggcg ctgtgctttt gcagatcagc
     2101 gcctgcgctg cccgacaact gggccgcaag gcccgtgcga cccgccgctt gcccgtttac
     2161 ttttactgaa agctcggcca cggaaaacag ctgccgttcc agttccgcgg tcaacaaccc
     2221 ttctacgccg attttgagat cgagcttcgg ctcgttggcc ctgacagccg cgccaagcga
     2281 aaaacgggtc ggaatcccgg gtgcgacacg cccagttttc aggtcgaaat ccgagatctc
     2341 atacgtcgca cccgtcatct gatccttcca tgtgagattc gcgctgctaa tcgttacccc
     2401 ttcgatctcg agcttgaccg gcattggtgc ctttgcgggt tccgcaggct tgccggatgc
     2461 ctggttgctc ttgccgtcac ttttcttgtc gccgccgagc aagtcatcga agttagtccg
     2521 gccatccttt tgcttcacca gattggcacg cagcccggcg agccgcacct cgtcgatgac
     2581 gatctgcctg gacaggagcg ggatcagcgc cagggagatc cgcatgtcgt caagatttgc
     2641 aaaaacggtt tcgcctctga cttctgacaa gcgggttttg cccaggtgaa tgcccagttt
     2701 cggaaacagc atgagacgaa tgtcgccttc aatggttaaa gtgcgttgcg tcttgtcctt
     2761 cacaaacgtg gccgcctgcg tcttgtattg attcgggtcg aatgttgcgg cgatgaacgc
     2821 cagcgcagcg gccagcgcaa gcacaacggc gcccgccgcg atcaggaccc acttcagagc
     2881 cttcatggaa tctatcccga gttcaaattt taaggacgcc gcattatcgc gcccgcaagg
     2941 cggatgccgc taccgttgtg catcaccact gcggcagttt tcctgtttag tcagcgaccg
     3001 gttggctaac cacaccggtt gcgccaactg aaaactggag cgggtgaagg ggatcgaacc
     3061 ctcgtctaaa gcttgggaag ctttcgttct gccattgaac tacacccgca cacggcgaat
     3121 tctacgacag gttgcctgaa atacgagcgg tgggcaggca cacttgcgtc ttaccactga
//
LOCUS       DATMZQ010000016         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3118947, whole genome shotgun sequence.
ACCESSION   DATMZQ010000016 DATMZQ010000000
VERSION     DATMZQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118947"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..448)
                     /locus_tag="VN496_00585"
     CDS             complement(83..448)
                     /locus_tag="VN496_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15464.1"
                     /translation="MKNIFLLCLALAMGACAHTPRLTTDQQFEAYRMQLRAERDTGKI
                     TVVEEQQKLRDHFWQVYGKDPNSTGHFAFSVSLMRSVQAGSFPLEDADALIAAKEADI
                     FATKIASRQRDCTYDCPWH"
     gene            complement(509..>2252)
                     /locus_tag="VN496_00590"
     CDS             complement(509..>2252)
                     /locus_tag="VN496_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF15465.1"
                     /translation="PVMSRYLLLEDEHGDGRSAMARLHTFVETNYERLRNRYLSALRW
                     ALVSRLTVFALFIVLVIGSAGAVMFIGWEFFPVVDAGQIRLHVNAPAGTRIEDTGVLF
                     SKVEDEIRRTIEAKDLDVVIDNIGIPPSTNLAYSDNVTLSSGDGEILVSLKPEHKVAT
                     REYLRRLRQVLPEKFPECTFYFQPGDMMNQILNFGLPAPIDVKIIGGDYKNFQLAKQI
                     EARMKRIAGAVDVHVHQIMNQPALRVEVDRTRAAQLGVTQREIAENFLIASSSSVVVT
                     PNYWNDPKTGRPYQVVVVQPHETALNSVEAVLNIPLSGKGQSPTQTLGNVATVRHVEL
                     PAIINHVNTELAFDVYANVQGRDLGAVASDVRKVVNEFIPKAREQKNNTRIEIAGQAE
                     SMYDAFTRMGIGLIAAVLLVYFIMVINFQSWTDPFIIITALPGAFCGIIWMLFLTGTS
                     FSVPSLMGAIMSVGVATANSILMVSFANEQLRNGMSPIDAALEAGATRLRPVLMTAAA
                     MVIGMAPMASGLGEGGEQNAPLGRAVIGGLILATVATLFFVPVVFSLLRRHGVPKARF
                     ADDDGVIATEGARR"
BASE COUNT          469 a          665 c          644 g          474 t
ORIGIN      
        1 cgatcccgac cgccaaaaaa ccggatcctc ccatttgcca ttccccaagg cctcgaatgg
       61 caaatgataa tccgggattg gtttaatgcc atggacaatc gtaggtgcag tcgcgttgcc
      121 gcgaagcgat ctttgtcgca aaaatgtcag cttccttggc agctatcaaa gcatcggcat
      181 cctccagcgg aaagctccca gcctggacgc tgcgcatcag cgagacggaa aaagcgaaat
      241 gaccggtgct gttggggtcc ttgccataca cctgccaaaa atgatcacgc agtttttgct
      301 gctcttccac cacggtaatc ttgcccgtat cgcgctctgc ccttaactgc atccgatacg
      361 cctcgaattg ctgatcggtg gtaagacgag gagtgtgcgc acatgcgccc attgccagtg
      421 caaggcacag cagaaatatg tttttcatga ttgctccttt gtgaaaaatc gattgcagat
      481 tgcctaaact tgacagcacc taaaatcttt atcgccgcgc accctccgta gcaattaccc
      541 cgtcgtcatc ggcaaatctc gccttcggca caccatggcg ccgcaacagg ctgaacacga
      601 cgggtacaaa gaacagcgtg gcaaccgttg ccagaatcag gccgccaata acggctcggc
      661 ccagcggcgc gttttgctcg ccgccttcgc cgagtccgct tgccatcggc gccattccga
      721 tcaccatggc cgccgcggtc atcagcacgg gacgcagccg cgtggctccg gcctccagcg
      781 cggcatcgat cgggctcatt ccgtttcgta gttgctcgtt ggcgaacgac accatcaaga
      841 tgctgtttgc agtggcgaca cccacactca tgatcgcgcc catgagggac ggcacgctga
      901 aactggtgcc ggtcaggaac agcatccaga tgattccgca gaaagcgccg ggcagcgccg
      961 tgatgatgat gaatggatcg gtccaggact gaaaattgat gaccatgatg aaatacacca
     1021 gcaatacggc ggcaatcagt ccgatgccca tgcgcgtgaa agcgtcgtac atgctttccg
     1081 cctggccggc gatttcgatg cgcgtgttgt tcttctgttc gcgggctttg gggatgaatt
     1141 cgttcaccac cttgcggaca tcggacgcca ccgcgccgag atccctgccc tgcacgttgg
     1201 catacacgtc gaacgcgagt tcggtgttga cgtgattgat aatcgcgggc agttccacat
     1261 gtctgacggt cgcgacgttg cccaatgtct gcgttggcga ctggcctttg ccggacagcg
     1321 ggatgttcag cacggcttcg accgaattga gtgccgtctc gtgcggttga accacgacca
     1381 cctgataggg ccgcccggtc ttcgggtcgt tccagtagtt tggcgttacg acaacgctcg
     1441 acgaagacgc gatcaggaag ttttccgcga tctcgcgttg cgtgacgccg agttgcgcgg
     1501 cgcgcgtgcg gtccacttcg acgcgcaacg ccggttggtt catgatctga tgcacatgca
     1561 cgtcaacggc accggcgatg cgcttcatgc gtgcttcgat ctgttttgcc agctggaaat
     1621 tcttgtaatc gccgccgatg atcttgacgt cgatcggcgc tggaaggccg aaattcagga
     1681 tctggttcat catatcgcca ggctggaaat agaacgtgca ttccggaaat ttctcgggaa
     1741 gcacctgtcg caggcgccgc aggtattccc tggtcgcgac cttgtgctcg ggtttgagcg
     1801 aaaccaggat ttcaccgtcg cctgaagaca acgtgacgtt gtcgctgtac gcaaggttgg
     1861 tcgacggcgg gatgccgatg ttgtcgatga cgacatccag gtccttggct tcgatggtgc
     1921 ggcggatctc gtcttctacc ttcgagaaga gcacgccggt atcctcgatg cgcgtgcccg
     1981 ccggggcatt gacgtgcagg cggatctgac cggcgtcgac caccggaaag aattcccagc
     2041 cgatgaacat cacggcgccg gcggagccga tcaccaaaac gatgaacagc gcgaatacgg
     2101 taagccggct taccagggcc cagcgcagcg cggaaagata gcgattgcgc aggcgctcgt
     2161 aattggtttc gacaaaggta tgcagccgtg ccatcgcgct gcggccgtcg ccgtgttcat
     2221 cctccagcaa cagatagcgg gacatcaccg gc
//
LOCUS       DATMZQ010000017         5504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1738189, whole genome shotgun sequence.
ACCESSION   DATMZQ010000017 DATMZQ010000000
VERSION     DATMZQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738189"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VN496_00595"
     CDS             <1..1275
                     /locus_tag="VN496_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXF15466.1"
                     /translation="ASGMIKRAPISLAMIYLAIGWLFGRSGWVVLDPGQHAKLLETLA
                     EIAVIVSLFSAGLKLRVRLRSPIWRLPIALATVSMAIAIGMMTFYGVAALALPIGVAV
                     LLGSILAPTDPVLAADVQVSDPGDTDALRFALTGEAGFNDGSAFPFVIIGLGLIGLHD
                     FYPRDWHWWTFDVVWPVAGGLVLGALLGWAIGRLVVYLRRRHLEAVGYDDFLALGLIA
                     SSYGLAIALHANGFLAVFATGLALRTVERELTGKEVPSDMSQIVGTDEEIASDDEHAP
                     AYLAAAVLSSNEQLERIGQLTLVVITGALLSQTGISLPAIATALFLFLIVRPLSVGPF
                     AMMAGMTLRQATMVSWFGIRGVGSLYYLFHVMNRGLPADDAAWMMHTVLCIIAASVVL
                     HGISVTPLMRRYANDAAPENSSCNKVSQKQPL"
     gene            1418..1882
                     /locus_tag="VN496_00600"
     CDS             1418..1882
                     /locus_tag="VN496_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15467.1"
                     /translation="MAGNSTLDPDNFPVNRSARKQKGHDTRSLGPSDSSDSGSDMAGP
                     GLIDDDAINLDRGTNEDTEAGRDDVADAGASIGDLQMDDNSDRYGTGEHLTAGKDPAV
                     KVDGDIDTDRIVGAEEAGLGGGLDQAEEAQLGVTDEELEAKRARSKVRQRER"
     gene            2085..2396
                     /locus_tag="VN496_00605"
     CDS             2085..2396
                     /locus_tag="VN496_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15468.1"
                     /translation="MNASLTEKDFEVIRGASNIANAIRNKLVKRGRRQEEIALEWLPD
                     PRFASIPEAPELKAEVAETGCVQQMFSREELEVSSQQLDRDSVVAKIDLVVEALQAGL
                     P"
     gene            2586..4646
                     /locus_tag="VN496_00610"
     CDS             2586..4646
                     /locus_tag="VN496_00610"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HXF15469.1"
                     /translation="MTDLPPLRSSLWRSPQYVGRVALLATVYFVVAKAALLLAIPPGY
                     ATAVWPPSGIALAAVLLAGARIWPGVWLGAALTNLTIQGSPLLAVLIGTGNTLEAVVA
                     AALVRRYINAHGEFESGEAAVMFAAMSALSALIAATIGTVSLTLAGTLNSSGFGASAW
                     IWFEGDFTGMIIVAPLILSWRLRPLPTWNTNKSLEGAVLAISMVSVTVFIFRNLVSAG
                     VLPLVFITLPFVLWAAIRFEQRVVAAAIAAMSAISVWYMLQAEGQFGPGFAKVSSFFL
                     LSYTSTLMMTGLVMSAIIGQRKRAELAVRQRVDELQASERHINEFLAMLSHELRNPLA
                     PMVNALALIRKVPATEHSPLLGIIERQVAHLSHIVDDLLDVSRITRGKILLQKALVDL
                     KDIVLRAVEASRPLIAAHGHAVESHLPDEQLLVDVDATRISQVVLNLINNAVKYTPAG
                     GSIAISVSRESGYALLKVRDSGIGIGPELIPKVFDLFMQGDRALDRSEGGLGIGLTIA
                     RRIVEMHGGSIDVSSGGPGNGSEFSVRIPLAQASASAVPVASRDSGAAPRAVRRRLLI
                     VDDHRENADTLATLLASLGHEVFTAYDGRKAIELAQQLHPDAVLLDIGLPGMNGYEVA
                     RALRNSQGMRMFLIAVSGYGQDEDRRRSREAGFNYHLVKPVDPARLGDIIDSLGADQI
                     AENN"
     gene            4714..>5504
                     /locus_tag="VN496_00615"
     CDS             4714..>5504
                     /locus_tag="VN496_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15470.1"
                     /translation="MKLNRRAFRVRAGMSKGLQTEVPLPTRLVSNEEFPPLPQTPAQR
                     EVEQRILSAAGRLAPRLGLTRREFLRTSGGMAIAFLAMNSVFGRFFEVSEAEAAEPAA
                     RNIAAGAGNPPFIFDVQLHYVGASYDPANAEAGRKGAVSKGALRNLRTSAKKMNPALA
                     ADSGSMADLSWQNFVKEVFFDSETSIGLISTPPGPYPQEAVVPPREMTHIRDEINRVT
                     QSRRMLAHGLVTPQLGRADLDFMQMQAETLKVDAWKAYTGSCPKG"
BASE COUNT         1101 a         1555 c         1686 g         1162 t
ORIGIN      
        1 gccagtggca tgatcaaacg agcgccaatc agtcttgcaa tgatttatct cgcgatcggc
       61 tggttattcg gtcgatccgg ctgggttgtg ctcgatccgg ggcaacacgc caagctgctg
      121 gagacgctcg cagagatcgc agtgatcgtg tctttgtttt ctgccggcct gaagctgcgc
      181 gtgcgcttgc gctctcccat ctggcggctg ccgattgcgc tcgcaacggt ttccatggca
      241 attgccatcg gcatgatgac attctatggg gtggcagcgc ttgcgctgcc gatcggagtg
      301 gcggtcttgc ttgggtcgat cctcgcacca actgaccctg tgctggcggc cgatgtccag
      361 gtgtccgacc ccggcgacac cgatgcgctg cgttttgcat taaccggtga ggctggcttc
      421 aacgatggca gcgcctttcc gtttgtcatc atcggactgg gcctcatcgg actgcacgat
      481 ttttatcctc gtgattggca ctggtggacg ttcgacgtgg tgtggcccgt cgcgggcgga
      541 ttggtgctgg gcgcgctgct gggatgggcg attgggcggc tggttgtcta tttgcgccga
      601 cgtcatctcg aagccgtcgg ctacgacgat ttcctggcac tcggactcat tgccagctcg
      661 tatggccttg ccatcgcatt gcacgcgaat ggatttctgg ccgtgtttgc aaccggactc
      721 gcgcttcgca ccgtggagcg cgagctcacc gggaaagaag tgccctcgga catgagccag
      781 attgtcggca cggatgaaga gatcgcgagc gatgacgaac atgcgcccgc ctatctggcc
      841 gccgcggtgt tgagttcaaa tgagcagctg gagcgcatcg gccagctcac tttggtcgtg
      901 atcaccgggg cattgcttag ccaaaccgga atttcactgc ccgccatcgc aaccgcgctg
      961 tttttattcc tgatcgtgcg gccgctatcg gtaggaccgt ttgcaatgat ggcgggcatg
     1021 accctgcgcc aggcaaccat ggtttcatgg ttcggcatcc gtggcgtcgg ctcgctctac
     1081 tatctttttc acgtcatgaa tcgtggcctt ccggcggacg acgctgcgtg gatgatgcac
     1141 acggtgttgt gcatcatcgc tgcgtccgtg gtcctgcacg ggatctcggt tactccactg
     1201 atgcggcgct acgcgaatga tgcagccccg gaaaacagct cttgtaataa agtaagccaa
     1261 aagcagcccc tttgaccttc gagcctttct gcgcacgccg ccagttgctg gaacattgct
     1321 cgcaaccgcg ttgccaaaca ggcaaggcag agcgaatcag accgccttga tggaaagcag
     1381 tgggcgaagg cacggcaacc aacattctgg agacatcatg gcaggcaata gcacgctgga
     1441 cccggataat tttccggtca atagatccgc caggaagcaa aaaggacatg acacgcgctc
     1501 gctcggaccg agcgacagtt cggatagtgg ttcggacatg gccggtccgg gactcattga
     1561 cgatgacgcc atcaatctcg atcgcggtac gaacgaagac accgaagccg gaagagacga
     1621 cgtcgccgac gcaggagcca gcatcggcga cttgcagatg gacgacaaca gcgaccgtta
     1681 cggtacggga gagcacctga ctgcaggcaa ggatccggca gtaaaggtcg acggcgacat
     1741 tgacacggac cgtatcgtcg gagcagagga agccgggctc ggtggcggtc tggaccaggc
     1801 agaggaggcg cagcttggtg tcacagacga agagctggaa gcaaaacgag cacgcagtaa
     1861 agtcaggcag cgagaacgct agggagccaa tagcgcttgt tgtgatgaaa gtacggccat
     1921 aacagcaaca ctgtaccggt aacaaaatgc ttccacgagc atgaacgtgg aacctgcaag
     1981 accagctggt gtcaatcgcc ctcgtttgaa ggggacggat cggagtgctc cgatttcctg
     2041 atttcccgag ggcacaacaa gacgcgcatg tacggaggag aacaatgaac gcatcgttga
     2101 cagaaaaaga ttttgaagtg atcaggggcg cgagcaatat agccaacgcc atccgtaaca
     2161 agctggtgaa gcgaggtcgg cgccaggaag aaatcgcact ggagtggctt cccgacccgc
     2221 gcttcgcgtc catcccggaa gcgccggagc ttaaagccga agtcgcggaa accggatgcg
     2281 tccagcaaat gttttcacgc gaggagctgg aagtatcgag ccagcaactg gaccgggatt
     2341 ccgttgttgc caaaatagat ctggtcgttg aagctttgca ggcgggcctg ccctgactgc
     2401 tgcgcgatcg cggcgttgcg taatagcgtt aatcaccatg gcgcaacgtt ggtagctccg
     2461 ttccaaacgg catcccatgg acccgtcccc tggctgtccc ggggttccgc gttcgcgatc
     2521 cccggcgggg ccgcaaccaa actgctttgt tgtgatggtc gcgaatacta ttacggacaa
     2581 cgtcattgac ggatttgcct ccattgcgca gcagcctatg gcgcagtcct cagtatgtcg
     2641 gccgtgtggc gttactggcc acggtgtatt tcgttgtcgc caaggcggcg ctgttgttgg
     2701 cgattccgcc cggttatgca actgccgtgt ggccgccgtc cggcattgcg ctcgccgccg
     2761 tgttgctggc cggtgcacgc atctggcctg gtgtctggct gggggcggca ctgaccaatc
     2821 tgaccatcca gggctcgcct ctgcttgcgg tactgatcgg cacgggcaac acgctggagg
     2881 ccgtggttgc cgcagcgctg gtgcgccgct acatcaacgc tcacggcgaa ttcgaaagcg
     2941 gcgaagcagc agtgatgttt gctgcgatga gcgcgctcag cgcgctcatc gcagcaacta
     3001 tcggcacggt ctccctgacg ctcgcgggca ctctcaacag ctcggggttc ggcgcgagcg
     3061 cctggatctg gttcgaaggc gatttcaccg gcatgattat cgtagcgccg ctcattctaa
     3121 gttggcgcct gcgtcccttg ccgacctgga acaccaacaa gtcgctggaa ggcgccgtgt
     3181 tggccatttc catggtttcc gtaaccgtat tcatcttcag aaacctggtg tccgccggcg
     3241 tgcttccgct ggtttttatc accttgcctt ttgttctgtg ggcagccatc cgattcgagc
     3301 agcgcgtcgt tgcggcggca atcgctgcga tgagcgcgat ttcggtctgg tatatgttgc
     3361 aggcagaggg acagtttggt cccggctttg cgaaagtgag cagcttcttt ttgctgtcgt
     3421 ataccagcac gctgatgatg accggtctcg tcatgagcgc aatcattggg cagcgcaagc
     3481 gtgcggaatt ggcagtccgg cagcgcgtcg acgaacttca ggccagcgaa cgccacatca
     3541 acgagtttct cgcgatgtta tcccatgagc tgcgcaatcc gctggcgccg atggtcaatg
     3601 cgctggcgct gatacgtaag gttccagcaa ctgaacattc gccgttgctg ggaatcatcg
     3661 agcgccaagt cgctcatctt tcgcatatcg tggatgattt gctcgatgtc agccgcatta
     3721 cgcgcgggaa gatattgctg cagaaagcgc tcgtcgatct gaaagacatc gtgttgcgcg
     3781 cagtcgaagc gtcgcgtccg ctgattgcgg cgcacggcca cgcggtggaa tcccacttgc
     3841 ctgatgagca attgcttgtc gatgtcgatg cgactcgcat ttcgcaggtt gtactcaatc
     3901 tgatcaataa cgccgtcaaa tacacgcccg ctggcgggtc aatcgcgatt tcggttagtc
     3961 gggaatccgg ctacgctctg ttaaaagtgc gggattcggg tatcggcatc gggccggaat
     4021 tgatcccgaa ggtgtttgac ttgttcatgc agggagatcg tgccctcgac cgcagtgagg
     4081 gcgggctggg aatcggcctg acaattgccc ggcgaattgt cgagatgcac ggtggttcca
     4141 ttgatgtgtc gagtggcggt cctggcaatg gcagcgaatt ttcggttcgg attccgcttg
     4201 cgcaagcgtc cgcatcggcc gtgccagtag cctcccgcga tagcggcgca gcgccgcgtg
     4261 ctgtgcgccg ccggctgctt atcgtcgatg atcaccgtga aaacgcggac acgttggcaa
     4321 cgctgctggc ttccctgggc cacgaagtct ttactgctta cgacggtcga aaagcgatcg
     4381 agttggcgca gcaactccat cccgatgccg tactgctgga tatcgggctg cccggcatga
     4441 acggctacga agtcgcccgt gcactcagga attcccaagg catgcggatg ttcctgatcg
     4501 cggtgtctgg atatggtcag gacgaagatc gccggcgcag tcgggaggct ggattcaatt
     4561 atcatttggt gaaacccgta gatccagcgc gacttggcga tatcatcgat tctctgggcg
     4621 cagatcagat tgcggaaaat aattgacccg gcatgcatca aacaagaacg gtccgcgttg
     4681 aatttccaac tctaaacatc caaaggagtt cagatgaaac tgaatcgtcg tgcatttcgt
     4741 gttcgcgctg gtatgagcaa gggcctgcaa acggaagtgc cgctgccgac ccggctggtt
     4801 tccaacgagg aatttccacc attgccgcaa acgccagcgc agcgcgaagt ggaacaacgc
     4861 atcctgtccg cggcaggtcg tctcgctcct cgcttggggc tcacccgccg ggaattcctg
     4921 cgtaccagcg gcgggatggc gatagcattc ctggcgatga attccgtgtt cgggcgtttc
     4981 tttgaagtat ccgaagcaga agccgcagaa ccggcggcga gaaacatagc ggcgggtgcc
     5041 ggcaatcccc ccttcatttt cgacgtgcaa ctccactatg tcggcgccag ctacgacccc
     5101 gccaatgcag aagctgggcg caagggcgca gtcagcaagg gcgcgttgcg caacctgcgc
     5161 accagcgcca aaaagatgaa cccggcgctg gcggccgaca gcggttccat ggcggatttg
     5221 tcgtggcaga acttcgtcaa ggaagtattc tttgacagcg agacgtcgat cgggttgatc
     5281 tccacgcccc cagggcctta tccgcaggaa gccgtggttc cgcccaggga aatgacgcat
     5341 atccgcgatg agatcaaccg tgtcacacaa tcgcgtcgca tgcttgccca cggcctcgtc
     5401 acgccgcagc ttggccgggc cgacctggat ttcatgcaga tgcaggccga aacattaaag
     5461 gtagatgcgt ggaaggctta caccggctcc tgtccgaagg gatt
//
LOCUS       DATMZQ010000018         6658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1387531, whole genome shotgun sequence.
ACCESSION   DATMZQ010000018 DATMZQ010000000
VERSION     DATMZQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387531"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /gene="aas"
                     /locus_tag="VN496_00620"
     CDS             <1..2084
                     /gene="aas"
                     /locus_tag="VN496_00620"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HXF15471.1"
                     /translation="HFSQPRLLIVSNHQSFLDGLILGAFLPVEPVFVVHTEITRNWFF
                     RQMLRLSDYLAVDPTNPMAMKRVVRLIESGRPVVIFPEGRITLTGSLMKVYDGPAFVA
                     ARTKAVIIPVRLDGAERSFFSRLSGSNPRVLLPAITVSIQPPASITVPNEGNGRARRR
                     KAGESMRKIMQEMIFRSHPVQTLYTALLDAIRIYGRGRKMVEDMRQTEYSYNALLKMT
                     LVLGLLVRRLSVRNETVGVLMPNLAATLCMVVGMTAFRRVPAMLNYTSGTDGMQSACV
                     AARISTIVTSRKFLDTARLQGQVDALRNVKIVYLEDLQPGVTVAVKLWALLHLFLPRL
                     LGRNLDPQSPAVVLFTSGSEGRPKGVVLSHSAILSNIAQVRAITDFSVEDRIFNALPI
                     FHSFGLVAGGLLPILTGIKLFLYPSPLHYRLIPEVIYDRSCTILFGTSTFLSNYAKFA
                     HPYDFYRVRYVVAGAEKLSQAVREIWYEKFGVRIFEGYGITETSPVVSVNSPMATRIG
                     SVGQLLPGMEARLMPVEGIERGGVLHVRGPNMMLGYLYYENPGVLVPPRSEVGAGWHD
                     TGDVVNIDEEGFVHILGRMKRFAKIAGEMVSLEVVEKIAVAASPNFQHAASSRPDERR
                     GEAIILFSTDPALIRERLQEAAQQAGSSELAIPKTIVHLPQIPLLGTGKVDYVKLKEM
                     PAVPSPALATA"
     gene            2115..4349
                     /locus_tag="VN496_00625"
     CDS             2115..4349
                     /locus_tag="VN496_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXF15472.1"
                     /translation="MTESPPDFSSLLQCLADRLPALRLALLKHAANDLALLRLAAGDV
                     LLRKGEKAEFLYVVVNGLLRATTIQEDGRELILSEFGPGEMAGEMAILAGGGIYSATV
                     SAPEGAAVVQVPRAVFERLATLEPAAIREMAGGIRRRLARDQLAIGLPRLFGPLDETM
                     LKFVEERVEWVRLHAGETLFSAGDKGEELYFVLGGRLRAVAPGGRTLSEMTRGESIGE
                     IALLTGDARTATVIAVRDSDLVRVSRRAFDEIIDKYPKVMQTIARIVIERLRQKETLS
                     AATKSGKCIAVLWAAELDPNVRFTERLVAALTRVGPTLHLSAPRVDKLLNRSGIAVAG
                     EDDAAGLRLTAWLDEQESHHQFLVYETDGNDTPWTRRCLRQADEILLVGKGSSDPSPG
                     ELERLVLGARDKISRAKQSLVLLHPDGQRLPSGTSRWFVGRNITRHFHIRLDSEQDFQ
                     RVARCLGEVAIGLVLGGGGARGLAHIGVVRALREAGVPIDMVGGTSMGAVMASLVAMG
                     QTKDQMLETNREAWLRKKPHKEYSLPFISLIRSRRLDSMARHIWGEVEMEDLWLGFFC
                     ISCNLSTSATVIHERGLLWKAIRASASLPGVFVPVLSDGDILVDGGLVNNLPGDVMRE
                     RSCRTVVVVDVGSEQKFTFGFPEFPSPWRLLGSRILPFAQRIEVPHIIDVLIRTTDVS
                     SSQKTRDVKKTADLCLRPPIDRYGVLQFELLDEIAEVGYHYTKQTLEELRARNSLPVI
                     FPSI"
     gene            complement(4438..4656)
                     /locus_tag="VN496_00630"
     CDS             complement(4438..4656)
                     /locus_tag="VN496_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15473.1"
                     /translation="MSNILTTLVIAAALSSAVVLAEEAVRTVAQNSQNGVSFTMHGKT
                     TCVLVDDKIFCAPATSPSQLNLASASLK"
     gene            4968..5198
                     /locus_tag="VN496_00635"
     CDS             4968..5198
                     /locus_tag="VN496_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15474.1"
                     /translation="MNRKLIATALLFLVSTAAVAARVESDEAQTYGTKANSPRKIIGA
                     VSSPESGRCDFGEPTSSQSGRETIGRAVCNQT"
     gene            5906..6556
                     /locus_tag="VN496_00640"
     CDS             5906..6556
                     /locus_tag="VN496_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXF15475.1"
                     /translation="MREVLRESAPLWSLSGEHIKALSERGTVKAYPKNTVIVNEGDRS
                     DTLFIILSGKVKAYLADEEGKEVLLSTQGPGEYFGEMILDQGPRSASVMTLEPCKFSV
                     VTTDQFKKFLTEHPDAGPELVMSLIHRVRELTKAVGNLALLDVYGRVAHLLLELATEE
                     NGEMVISERLTQQDMASRVGCSREMISRILKDLRTGGYVRMDGERIIIAKSPPRAW"
BASE COUNT         1410 a         1784 c         1988 g         1476 t
ORIGIN      
        1 cccatttttc gcagccccga ctgctcatcg tctccaatca ccaatccttt ctggacggat
       61 tgatactggg cgcgtttctg ccggtcgaac cggtgttcgt cgtgcatacc gagataacgc
      121 gcaactggtt ttttcgccag atgctgcggc taagcgatta tcttgcggtg gatcccacca
      181 acccgatggc aatgaaaagg gtggttcggc tgatcgagtc gggacgcccg gtcgtgattt
      241 tcccggaagg ccgcatcacg cttaccggaa gtctcatgaa ggtgtacgac ggtcccgcgt
      301 tcgtggctgc caggaccaag gcagtcataa taccggtgcg actggatggc gcggagcgtt
      361 cctttttcag ccgcttgtct ggcagtaacc cgcgcgtatt gctgcctgcg attaccgtat
      421 cgattcaacc gcccgcctcg atcacagttc ccaacgaagg taacggccgc gcaaggcgaa
      481 gaaaagcggg tgaaagcatg cgcaagatca tgcaggaaat gatcttccgt tcgcacccgg
      541 tacagacgtt gtacacggca ctgctggacg cgatccgtat ttatggccgc ggcaggaaga
      601 tggtcgaaga catgcggcag accgagtatt cgtacaacgc tttgctgaaa atgacactgg
      661 tgctcggatt gctggtgcgc cgtctcagtg ttcgaaatga aacggtcggc gtgctgatgc
      721 cgaatctcgc tgcgacactt tgcatggtag tcggcatgac agcatttcga cgcgtgccag
      781 ccatgctgaa ttacacatcc ggaacagacg gaatgcagag tgcgtgcgtt gcggcgcgta
      841 tctcaacaat tgtgacttcg cgcaaatttc tcgacacagc caggctccag ggacaagtgg
      901 atgcgttgcg caacgtgaag atcgtctatc tcgaagactt gcagcccggc gtcaccgttg
      961 cggtgaagct ctgggcactg ttgcatctgt ttcttcccag attgctaggg aggaatctgg
     1021 atccccaatc cccggcggtt gtcctgttta cctcggggtc cgaaggaagg cccaagggcg
     1081 tggtcctgtc gcactctgcc attctttcga atattgcgca ggtccgtgcc atcaccgatt
     1141 tttcggtgga ggacaggatc ttcaacgcgc tgccgatctt ccactccttc ggcctggtcg
     1201 ccggtggctt gttgcccatc ctgaccggaa ttaagctgtt tctttatcct tctccgttgc
     1261 actatcgcct gatcccggaa gttatctacg accgaagctg cacgatcctg tttggcacca
     1321 gcaccttcct gagcaactac gcaaaatttg cccatcccta tgatttttac cgcgtgcggt
     1381 acgtcgttgc aggcgctgaa aagctgtcgc aggcggtacg cgagatctgg tacgaaaagt
     1441 ttggtgtgag gattttcgaa ggttatggca ttaccgagac ttcgccagtc gtatcggtca
     1501 attctccgat ggcgacgcga atcggcagtg tcggccaact cctgccggga atggaagccc
     1561 ggctgatgcc ggttgaaggc atcgaacgcg gtggcgtttt gcacgtgcgt ggtccgaaca
     1621 tgatgctggg ttatctgtat tacgaaaatc caggggtgct ggtgccacca cgctcggaag
     1681 ttggcgcggg ctggcacgat accggtgacg tggtcaatat cgatgaagag ggctttgtgc
     1741 atatcctcgg ccggatgaaa cgcttcgcaa agattgccgg ggaaatggtt tcactcgagg
     1801 ttgtagaaaa aatcgccgtg gcagcgtctc cgaactttca gcatgctgcc tcatcgcggc
     1861 cggacgaacg ccgcggggaa gcgatcatcc tgttcagcac tgatcccgcg ctcatacgtg
     1921 aacgtttgca ggaagctgcg cagcaggccg gttcatcgga gcttgccatt ccaaaaacca
     1981 tagtgcattt gccgcaaatt ccgttattgg gtacgggcaa agtggactat gtgaaactca
     2041 aggaaatgcc ggccgtgccc tctccagcgc tggccacagc gtagtacggc aatgaagttg
     2101 aggagccctt cgccgtgacc gaatccccgc cggacttttc gagtttgttg cagtgtttgg
     2161 cggacagact gccggcacta cgtcttgctc ttctgaagca tgcggccaat gacctggcac
     2221 tgctaaggct ggcggctggc gacgtgttat tgcgcaaggg cgaaaaggcg gagtttctgt
     2281 acgtggttgt taatgggttg ctacgtgcca cgaccatcca agaggatgga cgagagttga
     2341 tcttgtcgga attcggtccc ggtgaaatgg ccggtgaaat ggcgatcctt gccggcggcg
     2401 gtatttatag cgcaacggta tcggctcctg agggggcagc cgtagtgcaa gtcccgcgcg
     2461 ctgttttcga acgtctcgcc accttggaac cggcggccat ccgcgagatg gccggaggaa
     2521 tacgacgtcg gctggctcga gatcagctcg caatcggtct accgcggctg ttcggcccgc
     2581 tcgatgaaac catgctgaag tttgtcgagg agcgtgtgga gtgggtccgg ctccacgccg
     2641 gggagacgct tttttccgca ggggacaagg gcgaggagct ctacttcgtg cttgggggac
     2701 gcttgcgcgc ggtggctcca ggcgggcgaa cacttagcga aatgacgcgc ggtgaaagca
     2761 tcggcgagat cgcgctgctt acgggtgatg cgcgcaccgc gaccgtgatc gccgtcaggg
     2821 atagtgacct ggtacgtgtg tccagacgtg cgtttgacga aatcatcgat aagtatccca
     2881 aagtaatgca gacgatcgcc cgcatcgtga tcgagcgcct gcgccagaaa gaaaccctca
     2941 gtgctgcgac aaaatccggc aagtgcatcg ccgtgctgtg ggccgcggaa cttgatccga
     3001 atgtgcgatt caccgaacgg ttggtggcag cgctgactcg cgtcggtccc acgctgcatc
     3061 tctcggcgcc ccgcgtggac aagctgctta accgttccgg aatcgctgtc gcaggtgagg
     3121 acgatgccgc cggccttcgt ctaacggctt ggctggacga gcaggagtcg catcaccaat
     3181 ttcttgttta tgaaacggat ggcaacgata cgccctggac gcgccggtgc ctgcgccagg
     3241 ccgatgaaat tttgttagtg ggcaaaggga gttcagaccc gtcccccggt gaattggaac
     3301 ggttggtcct tggtgcgcgg gacaaaattt caagagccaa gcaaagcttg gtgctgctgc
     3361 atcccgacgg gcaacgcttg ccatcgggaa cgtcgcgctg gttcgttggg cgcaatatca
     3421 cccggcattt ccatatacga ctcgactccg aacaggattt tcagcgtgtg gcgcgatgcc
     3481 tgggagaagt agcgatcgga ctggtgctgg gtggcggagg cgctcgtggc cttgcccata
     3541 ttggtgtggt acgtgccctt cgtgaagcgg gagtgccgat cgacatggtc ggcggcacca
     3601 gcatgggggc ggtcatggct tcgctggtgg caatgggaca gaccaaggat caaatgctgg
     3661 aaacaaatcg cgaagcatgg cttcgtaaaa aaccgcacaa ggaatattcg ttaccgttca
     3721 tttcccttat ccgcagccgc cggctcgata gcatggcccg gcacatctgg ggtgaagtgg
     3781 agatggaaga tttatggctg ggttttttct gtatttcgtg caatctctct accagtgcga
     3841 cggtaatcca cgagcgcgga cttttgtgga aagcgattcg cgccagcgct tctttgccgg
     3901 gagtgtttgt tccggtcctg agtgatggcg atattctggt ggatggcggg ctggtcaata
     3961 atcttccggg cgacgtaatg cgtgagcgtt cctgccgcac cgtggtggtg gtcgatgttg
     4021 ggtcggagca gaagttcact tttggtttcc cggaattccc gtcaccctgg cgactgctgg
     4081 ggagccgcat tctgccgttc gcgcagcgta tcgaagtgcc gcacattatc gacgtcctga
     4141 tccgcaccac ggatgtcagc agcagccaga aaacccgcga cgtcaagaaa actgccgatc
     4201 tctgtctgcg cccgccgata gaccgatacg gtgtcctgca atttgaattg ctcgatgaaa
     4261 ttgcggaggt cggttatcac tacacgaagc aaacattgga agaactgcga gcccggaaca
     4321 gtctgccggt aatattcccg tcaatctgat cccctgctat tgctggtggg atcgcgttgc
     4381 ctgcacaaaa aaagcccgcc aatcggcggg caaattccac gggagaatag ggacgagcta
     4441 tttcaggctg gcagaggcta ggttgagctg gctgggcgaa gtcgccggcg cgcaaaaaat
     4501 tttgtcatca accagcacgc aagtcgtttt gccgtgcatg gtgaagctca cgccgttctg
     4561 actgttttgc gcgaccgtgc gaacggcttc ttccgcgagg accaccgcgc tggacagggc
     4621 ggcggcgatc accaaagtcg tcaggatgtt gctcatggtc tgggtgctcc aagggcgtta
     4681 gtcacggaca ccactgtacg aatccccaaa tcgttccgaa ttgaattaat acgcaatccg
     4741 gatgtgagaa attcacgttg atcgatggcg gccgttggct gcatatagac cgatccgcgt
     4801 cttgttcgcc gttgggatcg gcagaaatcc gatggattca cacgaaactt gcctcggagc
     4861 ccgggtgtta atttttcaca ctcgggatat gcatttcgca cgggtgctgc tctggaccat
     4921 tgggtattct ggcctcacgt aatccgcgat acccacagga gaccaacatg aaccgcaaat
     4981 tgatcgcaac cgccctgtta tttctagtat cgactgcggc agttgcagcg cgagtagagt
     5041 cggacgaggc tcagacctac ggtacgaagg ccaattcgcc gcgcaagata atcggtgccg
     5101 tatcgagtcc cgaaagcggg cggtgcgact ttggtgaacc aacatcctct caatccggcc
     5161 gagaaacgat cggccgggcc gtgtgtaacc agacctagaa agcattacag cgtttttgca
     5221 gcccggaacc gcacgcggca cgggtccatc cggcgcaata aataggcagc gaaaggctga
     5281 tcccggtaat cctgctcagg acctgtccag caagccgggg tcacttcata ttcatgcgct
     5341 gcgcatattc tttgcagaat cgtatgccga ctgcatttcg tatatgcaca tcaggcacca
     5401 aataacgata agccgcgggc tgtttcagca aagtgatgtg ccctcctcaa cgcgtgaatt
     5461 tgacacagcc atcgcgcgta ccagttccgc gaaagattgt cagtcctgtc atagagtacg
     5521 gcgcacagga actgactaag gaaggtaaca tcatgaatac gacggtgacc aaaatgctta
     5581 ttgcggccgc gctcttgagt ctggtggtaa tcgcatgtga catgcccaag agcgccaaaa
     5641 tgaacggtgg gcactctgta gaatttgtgg aaagcaaaag caacatgcgc tgcgttttga
     5701 tagatggcca ggagttttgc tcgtcctcgc ggatgcggga accgattgaa aaatcgccta
     5761 ttgctgtgct ttagggattc acgccggttt tcgtcccgcc tcgttagcct tgtggtcgtg
     5821 cgacgataac ccggcttcct tgtaagagcg gcccgttaag agcacacttt ttgatgtacc
     5881 ggctgcagca caaggggaac agtccatgag agaagtattg cgcgagagcg ctccactctg
     5941 gagtttgtca ggggagcata tcaaggcgct ttccgagcgg ggaacggtca aggcctaccc
     6001 gaagaacacc gtcatcgtaa acgaaggtga tcgcagcgat acgctattca tcatcctgtc
     6061 cggcaaggtc aaggcctatc ttgccgatga agagggcaag gaggttctct taagcaccca
     6121 ggggccgggc gaatacttcg gcgaaatgat cctcgatcag gggccgcgct ctgcctcggt
     6181 aatgaccttg gagccttgca agttctctgt cgttacgaca gaccaattca agaaattcct
     6241 tacagagcat ccggacgcgg gccccgaact ggtcatgagc ctgatccacc gcgtacgcga
     6301 gctcaccaaa gccgtcggaa atctggcgct gctggatgtg tatggccgcg tagctcattt
     6361 gttgctcgaa ttggcaacgg aggagaacgg cgaaatggtg atcagcgagc gtctgacgca
     6421 acaggatatg gccagccgcg tagggtgctc gcgcgaaatg atcagccgca tcctgaagga
     6481 tctccgcacc gggggttatg tccgtatgga tggtgagcgg atcatcatcg ccaaaagtcc
     6541 cccgcgcgcg tggtagcggg aatgtccgaa gcgttcgcta tccgaggcgc catttccggc
     6601 aagtaatttg cgatatgaat tttcacgcca cgcggcgtga attttcacgg gactctgc
//
LOCUS       DATMZQ010000019         2720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2775283, whole genome shotgun sequence.
ACCESSION   DATMZQ010000019 DATMZQ010000000
VERSION     DATMZQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775283"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1083
                     /locus_tag="VN496_00645"
     CDS             37..1083
                     /locus_tag="VN496_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXF15476.1"
                     /translation="MRICVRSVAMLLAAAAMITAAASALAQETSGKVFELEPEYVYGR
                     DRRPQIGYRKPEVFDPLSDRLYQSGYRNFAPTAIPDELKTVPAMERVEKMHRILACAD
                     AWFWPFSRTAHNNEPAGIEVEILQAIAKRHGWDVSIAWVNTGMRFGVGVTFGTSVDKG
                     ICDIFMGLTITNDDHHMPRHKMAFTKPFMSTGYVLVTQGPASGVKTLDDAKTQNVKVG
                     VPAYSPMSEYADDHDIPHATFFQNYQVIDALIRGEVNAAMIWSGSISQAKLEHPEGEF
                     EMVKGYQPPDEMRFDNAWGIKAKEVELLKFIDEEFDQMLKSGETKRISERYGVPFFPP
                     IEPGAPNSPQNDER"
     gene            1255..1950
                     /locus_tag="VN496_00650"
     CDS             1255..1950
                     /locus_tag="VN496_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXF15477.1"
                     /translation="MMEKLIQLSMMSGGLLPLMGVLLLITLAVIVERSYFFFKVLKSG
                     HAMEHDLQLVEYQNWDKLEQLAAHYEGSLQRSIVSSALASRGKDPATMDRHIDEAIMW
                     QLPKIDRLIWVLDTSVTLAPLMGLLGTIIGMVHAFDILGTAGSSGAATQMVTGGIAEA
                     LVATGAGLFIAILAVLFLNHFNKRVRLAVHQMELLKVMVLNRLYGGGSTHQGMSLRDA
                     HSSAVTAIRGRQS"
     gene            1947..2363
                     /locus_tag="VN496_00655"
     CDS             1947..2363
                     /locus_tag="VN496_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXF15478.1"
                     /translation="MSARTRTGYFEALKPRVEVIPMIDIMMFLLVFFVVISLRMIAGT
                     GVPMEIPGSKTTQSIKSSTVTVGITRTGDTVIDGKPVNPEQLKARLVELKKQKPVDVV
                     LAGDKDVSLQSLLQVMDAVRGAGISSVGIAAKAEKK"
     gene            2386..>2720
                     /locus_tag="VN496_00660"
     CDS             2386..>2720
                     /locus_tag="VN496_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15479.1"
                     /translation="MSQAVVSRGRLWAQPGDTDQNTLPLWRALLIALGLEIILPFLLL
                     GINWSALQFWHEPPPEPVMSVRLEEPAPEEPRPEPPKKQKPKPERKIKQTPIVVPKPL
                     PNEVQSKIV"
BASE COUNT          620 a          749 c          805 g          546 t
ORIGIN      
        1 caggacccgg caggccttcc tggttgaaga atgtttatgc gaatttgcgt tcgcagcgtt
       61 gccatgctgc tggcggcggc tgccatgatc actgctgcgg cgagcgcact tgcacaggaa
      121 acctccggca aagtattcga actcgaacct gaatatgtgt acggtcggga tcgccggccg
      181 caaatcggct atcgcaagcc cgaggttttc gatccgctgt ccgaccgcct ctatcaatcg
      241 ggctaccgca atttcgcccc cactgcgatt ccagacgaac tcaagacagt gccggcgatg
      301 gagcgcgttg aaaaaatgca tcgcatcctc gcctgtgcgg atgcctggtt ctggccgttc
      361 tcgcgtactg cccacaacaa tgagccggcc ggcatcgagg tggaaattct gcaggcgatt
      421 gccaaacgac acggctggga cgtcagcatt gcctgggtga ataccgggat gcgcttcggc
      481 gtaggcgtca catttggcac gtcggtcgac aaaggcatct gcgatatttt catgggactg
      541 accatcacca acgatgacca tcacatgccg aggcacaaga tggcatttac caagccgttc
      601 atgagtaccg gctatgtgct ggtgacgcag ggtccggcca gtggcgtgaa aacgctcgac
      661 gatgccaaga cgcaaaacgt gaaggtcggc gtgccggcct attcgccgat gagtgaatac
      721 gccgacgacc atgacattcc gcacgcgact ttttttcaga actaccaggt cattgatgcg
      781 ttgatcaggg gcgaagtcaa tgccgccatg atctggtccg gttcgatttc gcaggcaaag
      841 ctggaacacc cggaagggga gttcgaaatg gtgaaaggct accagccgcc ggacgaaatg
      901 cgcttcgaca atgcctgggg catcaaagca aaagaagtcg agctgctcaa attcatcgat
      961 gaggaattcg accagatgct caaaagcggg gagaccaagc gtatctccga gcgctatggc
     1021 gtgccgtttt tcccgcccat cgaaccgggc gcaccgaata gtccgcaaaa cgatgaacga
     1081 tgaatagtga actgtgatcg gtgaacagtg aatggcgagc ggtgaatagt gattagtgaa
     1141 gggcgagcag cgtagcctaa ggaaagcttt ttctgttcgc gtctgcagat caccgttcac
     1201 agattaccat tcactgttcg gcaactcgaa gcacaacagg gagcgagagt aaaaatgatg
     1261 gaaaaactga tccaactaag catgatgtcc ggcggattgc tgccgctcat gggcgtgctg
     1321 ctgctgatca cgctggcggt gattgtcgag cgctcatact tctttttcaa ggtgctcaaa
     1381 tcgggccatg ccatggagca cgatctgcag ctggtcgagt accagaactg ggacaagctc
     1441 gaacagctcg ccgcgcacta tgaagggtcg ctgcagcgct cgatcgtgtc atcggcactc
     1501 gcttcgcgcg gcaaggaccc agccacgatg gatcgccaca tcgacgaggc aatcatgtgg
     1561 cagttgccca aaatagaccg gctcatctgg gtgctcgata cttccgtcac gcttgcaccc
     1621 ctgatgggac tgctcggcac gatcatcggc atggtgcacg ccttcgatat tcttggcacg
     1681 gcgggatcga gcggcgcagc gacccagatg gtcaccggcg gcattgccga ggcgctggtc
     1741 gccacgggag cgggtctgtt catcgcaatc cttgccgtgc tgttcctcaa ccatttcaac
     1801 aagcgcgtgc ggcttgccgt gcaccagatg gaattgctta aggtgatggt gctgaaccgg
     1861 ctatatggcg gcggctcgac gcatcagggc atgtcgttgc gtgatgcgca cagcagcgcc
     1921 gtcacagcca tacgcgggag gcaatcatga gcgcgcgcac ccggaccggt tacttcgaag
     1981 cgttgaagcc ccgcgtggaa gtcatcccga tgatcgacat catgatgttc ctgctggtgt
     2041 tctttgttgt gatctcgttg cgcatgattg ccggcaccgg tgtgccgatg gaaattccag
     2101 gttcgaaaac gacccagtct atcaagtctt ccactgtcac tgtcggcatc actcgcacgg
     2161 gcgacacggt aatcgatggc aagccggtca atccggagca gctgaaagcc cgcctggtcg
     2221 aattgaaaaa gcaaaagccg gtcgatgtgg tattggcggg agacaaggac gtgtcgctgc
     2281 aatcgctttt gcaggtgatg gacgcagttc gtggcgcagg aatttcttcg gtgggtatcg
     2341 ccgccaaggc ggaaaagaag tgaacgcaag aggcactgcc atttcatgag tcaggcagtc
     2401 gtcagccgcg gccgattgtg ggcacagccc ggcgacacag accaaaacac gcttccgttg
     2461 tggcgggcgc tgctgatagc gctcggtctg gaaattatcc tgccgttcct gttgctgggc
     2521 ataaattggt cggccctgca attctggcat gagccaccgc cggagccagt aatgtcggta
     2581 cgcctggaag aaccggcacc cgaagagccg cgacccgaac cgccgaagaa gcaaaaacca
     2641 aaacctgaac gcaagatcaa gcaaacgccc atcgtcgttc cgaagccgct gccgaatgag
     2701 gtgcagtcga aaatcgtgtt
//
LOCUS       DATMZQ010000020         9587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4149866, whole genome shotgun sequence.
ACCESSION   DATMZQ010000020 DATMZQ010000000
VERSION     DATMZQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149866"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1028
                     /locus_tag="VN496_00665"
     CDS             234..1028
                     /locus_tag="VN496_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15480.1"
                     /translation="MAATEHNFEPMTAGMVLDKSFRLYMDNFPLMVGLSAILNIPLLI
                     LPLLFGPGRLDPRNISITRVLTVLIGALVFLLAALIVTPLITGATTKAVSDIYLGNVV
                     TAGSSISAAWRKAWTLLKTQFVVGLIVGLGMLLLVVPGMLWMLSYALIAPVVMIEGSN
                     NGREIRQRSWSLVKGNRGKVFLIFFIIVVIQILAQAGIGIMAVFGLGTSNVSLISQIL
                     NGIVSILMYPMSAIAVTLLYYDFRIRKEGFDLEMLSHALGEPARGA"
     gene            1103..1657
                     /locus_tag="VN496_00670"
     CDS             1103..1657
                     /locus_tag="VN496_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HXF15481.1"
                     /translation="MTGAWIAQTLQAFFKWLSKLSDWAGKHPSSARILIYVLGIVLIV
                     LLAHIAYTVIGEFLSLRKRNAETDVRYRTLPALEGVAENWSDAFVLAKAALQSGDLYR
                     AMWITHRILLSVLDLREMVKFTRWKTNSDYIRECRAGDPAAATLREITAAYERVVYAH
                     GDFDREQAARLVEQVEAFAGKEVR"
     gene            1654..2799
                     /locus_tag="VN496_00675"
     CDS             1654..2799
                     /locus_tag="VN496_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HXF15482.1"
                     /translation="MNAARKNFWTAIVAAGICVVLLIVIAGLSINRATDFLHQRPSTF
                     FTDPTGARALLLVLQRVLPNAGQWRRPFMDLPSEAGSTRSTLIVMGPQEPLSESDATA
                     LDRWITSGGQLILATNMEWQIRKPNKAGEKEEFIPLGYLARHSLKVKPDATGNDAIAA
                     VTTKALGTGRIVFIPDSYAFSNESLGSTDDAVWIVSQVSEWGGAALIDEYHQGFGEKR
                     ELHTLVAMFAASPWGFACMQLALAGVVYILGCRRRFGRPVDELPVERTSPIEAVEALG
                     GLFETARAGALSVRTVHQYLNLQLTAMFGYSVDLSNPAVRDRIASRSSVSKAELESYA
                     EAVKRAIDGETTSDADLIRIARDATTISRSFSHGNARQHPGRPAAAG"
     gene            2753..3721
                     /locus_tag="VN496_00680"
     CDS             2753..3721
                     /locus_tag="VN496_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXF15483.1"
                     /translation="MEMPGNIQGGQPLLASLKTELARVIEGQEAVIESLMVALVAGGH
                     VLIEGVPGVAKTLMARSLASALSATFSRIQFTPDLMPADITGVNIFEPGTGSFRFRPG
                     PLFADIVLADEINRAPAKTQSALLEAMQENQVSIDGVTHNLSGMLTVLATQNPIEYEG
                     TYPLPEAQLDRFMIKVVVDYPALEAERLMIARMHRLGENVTHPESVIRPVISPAELLD
                     IRAASHRVDVDDSIVNYIVEITRNSRSLANLSLGASPRAGVMLLRAAKALAVLHGKSY
                     VTPDEVREMALPVLRHRVRLTPEAEIEGLTADGCIDTLMRQATVPR"
     gene            3721..5028
                     /locus_tag="VN496_00685"
     CDS             3721..5028
                     /locus_tag="VN496_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXF15484.1"
                     /translation="MVPTRRLVRWIALGSPLWLIALATPAGWGVAAAYLLVLASICVM
                     DYFAVPNASAFQVQRELGRFSLGAVTDVRIQIACRSKRLLRLSMRDELPPALRQLTPI
                     QNVNLPIHGECEWIYQVRSERRGKYHLDTIALRAGRTGALVKRQLRIAVPDDLRVYPR
                     FATADEYRLLARLHHQDEAMRRPRRVYGQGTDFESLLTYSPGEDLRNVDWKVSAKRGF
                     LVSRNLQTERGQQISVMIDGGRRMAEQIGEYSRFEYALNAAVMLSHVAQRRGDTVAVS
                     TFSDRVESFMPPTRGTAVMRTVLESLSTVEVRQVETDYWQVVGQVMDRLKRRSLLVMM
                     TDVLDAAGSSGLLINLARAASRHLVLCVVLTEPRIAEMAASEAETLQDAYVKAAASHM
                     QLQRHLALEKMRSRGIMVLETSPEHLTIQLIRRYLEVRRANLQ"
     gene            5028..6809
                     /locus_tag="VN496_00690"
     CDS             5028..6809
                     /locus_tag="VN496_00690"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HXF15485.1"
                     /translation="MDERISIITPDHVELDFEPAGIGSRLLAFIIDQIILYLSILAIV
                     LLLLFSGAMGIVSGISQEWSSTITLTVLAFVIFFLNWGYFLLFEALNKGQTPGKRSTG
                     IRVVRDSGLPVGWRESALRNFVRIADIMPPPACFVAGLMILLSKQGKRLGDLLAGTMV
                     VREDFGWSGRGSTARWEAAWIARAEKGRFGKSIVLADMKIEASQMQIIKRYLERRDSL
                     PLVQRQNLAWKIASPFLKAMGEDANDLQTRTDRYIVCERVLSDILNRANTTQPAVGAS
                     AGDSADSKREQWLAFDREISGIAKGGARGLRLLRPNHLVRIIGSYRQLAGDVARARSL
                     GRNSVLVRRLNNIAIRAHNVLYGNIRSRSRSGGPSWMVRFPIAVRGHAAAVMFSALML
                     FAPSAMTYVAVQLHPDLGYDLVPSGFLDFEPARDVSLHSFPGLARPVAASTIMTNNIQ
                     VTLLAFGLGMTAGLGTCLILISNGMQLGAVAGWMTARGSSRALWGWIMPHGGTELLAI
                     VLSGAAGFMLAGALIAPGNVRRAVALRLVAKDALAIILGVMGMLVVAGLIEGFVSPSS
                     IGYSARIAVLAITLTGWFAYLGLAGRK"
     gene            complement(6946..9216)
                     /locus_tag="VN496_00695"
     CDS             complement(6946..9216)
                     /locus_tag="VN496_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXF15486.1"
                     /translation="MIQIGAISPTPPQFEIDPVCKMRVLPETAAAQFSYNGKTYYFCN
                     PRCRDRFQSDPESFLSPGSKPEPPKSDTIYVCPMHPEVRQMGPGICPKCGMALEPEIA
                     KADEGPNPELLDMTQRFWIAAVVSVPVMVFGMLEIVPVLQLFLASVAVLYAGQPLLQR
                     GWNSVVNRSPNMFTLIGIGIGTAYVYSAVAVFATASQLPVYFEAASVITALVLLGQVL
                     ELRARSRTGSAIKSLLGLAPKTAHHVTAGGGEMDEPLELVKVGDELRVRSGEKIPVDG
                     IVSSGSSTVDESMISGEPIPVEKYPGAAVVGGTINGNGTFTMRAERVGRDTILAQIVH
                     MVGQAQRSRAPIQRLADVVSGWFVPAVIGAAAITFAIWAVAGPQPRFTTALLNAVAVL
                     IIACPCALGLATPMSIMVGIGLGATSGVLIKNAESLEILEKVDTIVVDKTGTLTEGKP
                     ALAAIVALAEFPEAEVLRLAASVERGSEHPLAAAIVQAASARGLAFAKVTGFQSKTGR
                     GVSASIDGRVVVLGNQRFMDELGLGSNSAFDGASGRAEQLRREGQSVMFVALDGKVAG
                     LIGVADPIKASAREAIGALRREGIQVIMLTGDSRTTAEAVARQLGLDDVRAEVLPDQK
                     GKVIQELQKKGHIVAMAGDGVNDAPALAQAQVGIAMGSGTDVAMESAGITLLRGDLRG
                     ICRARALSRATMKNIRQNLFLAFVYNTLGVPIAAGVLYPFFGILLSPMIASAAMTFSS
                     VSVITNALRLRRATLM"
     gene            complement(9304..>9587)
                     /locus_tag="VN496_00700"
     CDS             complement(9304..>9587)
                     /locus_tag="VN496_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15487.1"
                     /translation="FRSALMLVGVIGLLEVPGWAQCVMCGTALASSPEGRALASSFRY
                     GIVILMVAPYLILGSIGYAIFRAYRKRRDLMHPKALTVGAVYDRARSPR"
BASE COUNT         1939 a         2825 c         2794 g         2029 t
ORIGIN      
        1 cgaagggccg ggtgaggggt acagaaacgg caccgtctat tacccctcac ccggcccgcc
       61 agcctgctgg ctgcgggcca ccctctccct tcgggagagg aacgcgtccc caattcattc
      121 acaccttcag acgatggagc cctgcacgct ggagtgacat tgacggcctg agcctgtgtc
      181 gggtacagta gcgcggctcc cgattcattc tctcaagatc ccggaggcga cgagtggctg
      241 ctacggaaca caatttcgag ccgatgaccg ccggcatggt gctggataaa tccttccggc
      301 tctatatgga taatttcccg ctgatggtcg ggctctcggc gattttgaac attccacttc
      361 tgatcctgcc gcttttgttt ggcccgggaa ggctggaccc aaggaacatc agcatcacga
      421 gggttttgac agtcctgatc ggcgcgctcg tgttcctcct cgcagcgctt attgtcactc
      481 cgctcatcac aggcgcaacg acgaaggcgg tgagcgatat ctacctgggc aacgtcgtga
      541 ccgcgggatc ttccatttcc gctgcctggc gaaaggcatg gacgctactg aaaacacagt
      601 ttgtcgtcgg gctgattgtc ggcctgggaa tgcttctcct ggtggttccg ggaatgctat
      661 ggatgctctc ctatgctctt attgcgcccg tcgtcatgat cgagggatcg aacaatggcc
      721 gtgagatccg ccagcgaagc tggagtcttg tcaaaggaaa ccgcggcaag gtttttctga
      781 tatttttcat catcgtcgtg atccagattc ttgcccaggc cggtatcggc atcatggcgg
      841 tcttcggact cggtacaagt aatgtctccc ttatcagcca gatcctcaat ggcattgtct
      901 ccattttgat gtatccgatg tctgcgatcg cagtgacgct tctctactat gatttccgca
      961 ttcgcaagga aggattcgat ctggaaatgc tgagtcatgc actcggcgaa ccagcgcgag
     1021 gggcatgatt cagccggttt ctccggaaca tgttcgggaa atcacacaga ctgtattgaa
     1081 acggcctgag tttcgggaag acgtgacggg cgcgtggatc gcacaaacgt tacaagcatt
     1141 cttcaagtgg ctttcgaagc tgtcggattg ggcaggaaag catcccagtt cggcaagaat
     1201 cctcatctac gtccttggga tcgtcctcat tgtgctgctt gcccacatcg catacacggt
     1261 gatcggggaa tttctttctc tccgtaaacg aaacgctgaa acggatgtac gctaccgcac
     1321 cttgcctgcc ctggaaggcg tggcggaaaa ctggagcgat gcgtttgtgc ttgcaaaagc
     1381 ggcgctgcaa tccggcgatc tgtaccgtgc catgtggatc acacatcgga tcctcctctc
     1441 cgtgctcgac ctccgcgaga tggtgaaatt tacccggtgg aaaaccaaca gcgattacat
     1501 ccgtgaatgc cgggccggcg atcccgcggc ggcaacgctt cgggaaataa ccgcggctta
     1561 tgaacgcgtc gtctacgcgc atggcgactt cgaccgggag caggcggccc gccttgtcga
     1621 gcaggtcgaa gcgtttgccg gcaaggaggt ccgttgaacg cggcgcgaaa gaatttctgg
     1681 acggcgatcg tcgcggccgg catttgcgtc gtccttctca tcgttattgc gggactttcc
     1741 attaacagag cgacggattt cctacaccag cgtccgtcga cttttttcac ggatcccacc
     1801 ggcgcccggg ccttgcttct cgtgctgcag cgcgtgctgc caaacgccgg ccaatggcgg
     1861 aggccattca tggaccttcc ctcagaggca ggctccaccc gttccacgtt gattgtcatg
     1921 ggcccgcagg aaccgctcag cgagtccgat gcgaccgcgc tcgaccggtg gatcacgtcc
     1981 ggcgggcagc tgatcctcgc aaccaacatg gaatggcaaa tccgcaaacc gaacaaggcg
     2041 ggagaaaagg aggaattcat tcccctggga tatcttgcaa ggcacagcct gaaagtgaaa
     2101 cccgatgcta caggcaatga tgccattgca gccgtcacca caaaggcgct gggcacaggc
     2161 cggatcgtat tcataccgga ctcgtatgcc ttctccaacg agtcgctggg ctccacggat
     2221 gacgcggttt ggatcgtctc gcaagtatcc gaatggggag gcgctgcgct aattgacgag
     2281 taccatcagg gcttcgggga gaagcgtgaa cttcacacgc tggtggcgat gtttgccgca
     2341 tcgccctggg gattcgcctg catgcagctg gcgcttgccg gagtcgtcta tatcctgggc
     2401 tgcagacgcc gattcggacg ccctgtcgat gagcttcccg ttgaacgcac cagtccgatc
     2461 gaagctgtcg aggcgcttgg aggtctgttc gaaaccgcac gggcaggcgc cttatcggtg
     2521 agaacagttc atcagtatct caatctgcaa ctcacggcaa tgttcggcta ctccgtcgat
     2581 ctttcaaacc cggccgtacg ggatcggatt gccagccgaa gttcggtgag caaggcggag
     2641 ctggaatcct atgcggaagc ggtgaagcga gcgatcgacg gagaaacgac atccgatgcg
     2701 gacctcattc gaatcgcccg tgatgcaaca acgatctcaa ggagttttag tcatggaaat
     2761 gcccggcaac atccagggcg gccagccgct gctggctagt ctgaaaacgg aactcgcgcg
     2821 cgttattgaa ggccaggagg ccgtcattga aagcctgatg gtggcgttgg tcgccggcgg
     2881 acatgtactg atcgagggcg tcccgggcgt cgcgaaaacc ctgatggcgc gcagcctggc
     2941 ttccgccctg tcggcaacgt tttctcgcat tcaattcacg ccggacctga tgccggccga
     3001 catcaccggc gtgaacatat tcgaacccgg aaccggttcg tttcgatttc gtccaggccc
     3061 gctgtttgca gatatcgtac tcgccgacga aatcaatcgc gcccctgcca agacccagtc
     3121 tgctttgctt gaggcgatgc aggaaaacca ggtgagcatt gacggtgtca cacacaacct
     3181 ttccggcatg ctgacagttc ttgcaaccca gaatcccatt gaatacgagg gaacgtatcc
     3241 acttccggaa gcccagctcg accgcttcat gatcaaagtg gttgtcgatt atcctgctct
     3301 cgaagccgaa cgtttgatga ttgcccgcat gcatcggctt ggagaaaacg tgacgcatcc
     3361 ggaatccgtg atacggcctg tgatcagtcc cgcggaactg ctagacattc gcgccgcttc
     3421 ccatcgcgtg gacgtggacg attcgatcgt gaattacatc gtcgaaatca cccgcaattc
     3481 ccgttcgctc gccaacctga gcctgggcgc aagtccgcgt gcgggggtca tgctgctgcg
     3541 tgccgctaaa gccctcgccg tgcttcatgg aaagtcgtac gtcacgccgg atgaggttcg
     3601 tgaaatggcg ctgcccgttc tccgtcaccg ggttcgtttg actcctgaag cggaaattga
     3661 aggtcttacc gccgatggat gcattgacac tctgatgcgg caggccacgg tgccccggta
     3721 atggttccca ctcgacggct cgttcgctgg atcgcactgg gatcgccact ttggttgatc
     3781 gcgctcgcca cgccggccgg atggggcgtg gcagcggcat acttgctggt gctggcttcc
     3841 atctgtgtca tggactattt cgccgtgccc aacgcatcag catttcaagt gcagcgtgaa
     3901 ttgggccgat tttcacttgg agccgtcacg gacgttcgga ttcagatcgc gtgccgttcg
     3961 aagcgcctgt tgcgtttgtc catgcgcgac gaactgccgc cagccctgcg tcagctgaca
     4021 ccgattcaga acgtcaatct ccccatacat ggcgaatgcg aatggattta ccaggtacgt
     4081 tcggagcggc gcggcaaata ccatctcgat accatcgcct tgcgggctgg ccgcaccggc
     4141 gcactggtca agcgccaact acgaatcgcc gtgcccgacg atctgcgtgt ctatccccga
     4201 ttcgcgactg ccgatgagta tcgattgctc gcccgccttc atcatcagga cgaagccatg
     4261 cggcgtcccc gccgcgtcta cggacagggc acggatttcg aaagcctgct gacctattca
     4321 ccgggtgaag atcttcggaa tgtcgactgg aaagtcagcg ccaagcgcgg ctttctggtg
     4381 tcacgcaatc tgcagactga acgcggccag cagatctccg tcatgatcga tggcgggcgg
     4441 cgcatggccg agcagattgg cgagtattcc cgattcgagt acgctctcaa cgccgcggtt
     4501 atgctcagtc acgtggcgca gcgccgaggt gatactgtcg ccgtatcgac attctcagac
     4561 cgcgtggagt ctttcatgcc gcccacacgc ggcacggccg tcatgcggac tgttttggaa
     4621 tcgctgtcca ctgttgaagt gcggcaggtg gaaaccgact actggcaagt tgtcggacag
     4681 gtcatggacc ggctgaagcg ccgcagcctt ctcgtcatga tgacggatgt tttggatgcc
     4741 gccggcagtt cgggactcct catcaatctt gctcgcgcgg cctcgcgtca cctggtgtta
     4801 tgtgtggtgc tgacggagcc gcgcatcgca gagatggccg catcggaagc agagacgctg
     4861 caggatgcct atgtcaaggc cgccgcatct catatgcaac tgcagcggca cctggcgctg
     4921 gaaaagatgc gaagccgggg catcatggtt ctggaaactt caccggagca tctgacgatt
     4981 caattgatac gccggtacct cgaagtccgg agggcgaatc ttcaataatg gacgaacgca
     5041 tttccatcat cactcccgat cacgtggagc tcgatttcga gccggcaggc attggatcac
     5101 gcctgctcgc attcatcatc gatcagatca tcctgtatct cagcatcctc gcaatcgtcc
     5161 tcctattgct gttttcgggc gcaatgggca tcgtgtcggg catctctcag gaatggtcca
     5221 gtaccatcac actaacagtg ctggcattcg tcattttctt tctgaattgg ggatacttcc
     5281 tcctgttcga agcgcttaac aagggacaga cgccgggcaa gagatcgacc ggaattcgcg
     5341 tggtccgcga cagcggcctg cccgtcggct ggcgcgagtc cgcactgcgc aattttgtcc
     5401 gcattgcaga catcatgccg ccaccggcct gttttgtcgc gggactgatg attctgcttt
     5461 caaagcaggg gaagcggctt ggagatcttc tggccggaac catggtcgtg cgagaggatt
     5521 tcggatggag cggacgcggc agcacggccc ggtgggaagc cgcatggatt gcgcgcgcgg
     5581 aaaaggggcg gttcggaaag agcatcgtac ttgcggatat gaagatcgag gcgagtcaga
     5641 tgcaaatcat aaagcgctat ctcgaacgcc gcgattccct gcctctcgtc cagcggcaga
     5701 atcttgcgtg gaaaattgcg tctccgtttt tgaaggcaat gggagaggac gccaacgacc
     5761 tgcaaacccg tacggaccgg tatatcgtgt gcgaacgtgt ccttagcgac atcttgaatc
     5821 gggcaaatac cacgcaaccg gccgttggag cttctgccgg cgactccgcc gactcaaagc
     5881 gcgaacagtg gctggcgttc gatcgcgaaa tttccggcat tgcaaaaggc ggagcgcgtg
     5941 gattgcgttt gcttcgaccc aatcatctgg ttcgaatcat cggaagctac cgccaactgg
     6001 caggcgacgt ggctcgagcg cggtcgctgg gacgaaactc cgttctggtt cgccgcctga
     6061 ataacatcgc aatccgcgcg cacaacgtat tatatggaaa catccggtcg cgaagccgct
     6121 ctggcggccc ttcgtggatg gttcgcttcc cgattgccgt gcggggccat gcggccgcag
     6181 tgatgttttc agcgctcatg ctctttgctc cttccgcgat gacatacgta gcggttcaac
     6241 tccacccgga cctgggctac gatctggtcc catcgggatt tctcgatttc gagccggcac
     6301 gcgatgtgtc gctgcacagc tttccaggcc ttgcacggcc tgttgcggca tccacgatca
     6361 tgaccaacaa tattcaggtc actctgctgg cgtttgggct ggggatgact gcagggttgg
     6421 gcacgtgtct gatactcatc tccaacggta tgcaactcgg cgccgtcgct ggatggatga
     6481 ccgctcgagg cagcagccgt gctctctggg gctggatcat gccccacggc ggaaccgaac
     6541 tgcttgcgat cgtattgtcc ggcgctgcgg gattcatgct ggccggcgcg ctgattgctc
     6601 cgggtaacgt tcggcgcgcg gttgcgctgc gtctggttgc aaaggatgca ctggccatca
     6661 ttctgggtgt gatgggaatg ctcgtggttg caggcctgat tgaaggtttc gtctcaccga
     6721 gcagcatcgg atattcggca cggattgccg ttctggccat cacgctgacg gggtggtttg
     6781 catacctggg tcttgccggg aggaagtgat tgggggcggc tgtaggcgcg gtctatgacc
     6841 gcgcacaatc cttcacctca atttgtttgt agacgataga gtgtgcgcgg tcatagaccg
     6901 cgcttacagc tattcggatg cgcttcgcgc accatattac cgcacctaca ttaacgtcgc
     6961 ccgccgcaag cgaagcgcgt tggttatgac ggaaaccgag ctgaatgtca tggcggcgct
     7021 ggctatcatg gggctgagca ggattccaaa aaacggatac aacacgccgg ccgcaatcgg
     7081 aacgccgagc gtgttgtaca caaatgcgag aaagagattc tgacggatgt tcttcatcgt
     7141 tgcgcggctc agcgcgcgcg cgcggcaaat gcctcgaaga tctccgcgaa gcaacgtgat
     7201 tccggcgctc tccatggcca catcggtgcc gctccccatc gcaatgccaa cctgagcctg
     7261 cgcgagcgcg ggtgcatcgt tgacgccgtc gccggccatg gcaacaatgt gacctttctt
     7321 ttggagctcc tgaatcacct tccctttttg atccggaagg acttccgcgc gaacatcgtc
     7381 gagacccagc tgccgcgcaa cagcttctgc cgtggtgcgg ctgtcgccag tcagcatgat
     7441 gacctgaatg ccctcccgcc ggagcgcgcc gatggcctca cgggcggaag ccttgatggg
     7501 atcggcgacg ccgatcagtc ctgcaacctt cccatccaat gccacgaaca tcacgctctg
     7561 gccttctcgc cgcagttgct cggctcgccc ggacgcacca tcgaaagcgc tgttggaacc
     7621 tagtccgagt tcatccatga atctctgatt gcccaatacc accacgcgcc catcgatcga
     7681 cgcgctgacg ccgcgtccgg ttttggattg aaagccagtc actttcgcga acgccaagcc
     7741 tcgcgctgac gccgcctgta cgatcgcggc ggcaagcgga tgctcgctgc ctcgttccac
     7801 gctggccgcc agtcgaagaa cttccgcttc cggaaactcc gcaagggcta caatcgctgc
     7861 gagagcgggt ttgccttcgg tcagcgtgcc ggtcttgtcg acaactatcg tgtcgacctt
     7921 ctccagaatt tccagagact ccgcattctt tatcagcacg ccggaggtcg cgccgaggcc
     7981 gatacccacc atgatggaca tcggcgtcgc caggccgagc gcgcaggggc aggcgatgat
     8041 caacacagcc acggcattca ggagtgcggt cgtgaacctg ggctgaggac ctgcaacggc
     8101 ccatatcgca aatgtaattg ccgcagctcc aatcacggcc ggaacaaacc atccggaaac
     8161 gacatcggcc agccgctgaa ttggcgcacg gctgcgctgg gcctgcccaa ccatgtgaac
     8221 gatctgtgcc aggatcgtgt cgcggcccac gcgctcggcc cgcatggtga aagtcccgtt
     8281 gccattgatc gtgccgccaa ctactgctgc gcctggatac ttttcgaccg gaatgggttc
     8341 gccggaaatc atggattcat cgaccgtgct ggatccactc gagacgatgc cgtcgactgg
     8401 aatcttctcc ccggatcgaa cgcgcaactc gtctccaacc tttaccaatt ccagcggctc
     8461 atccatttcc ccgccgcccg cggtaacgtg gtgcgcggtt ttgggcgcga gtccaagcag
     8521 actcttaatg gcggatccag ttcgcgagcg tgcgcgcagc tccagtacct gaccgagcag
     8581 caccagtgcg gtaatcaccg aagccgcttc aaagtacacc ggcagttgcg acgcggttgc
     8641 gaaaaccgca actgcgctgt aaacgtaggc ggttccaatg ccaatgccga tcaaggtaaa
     8701 catgttcggg cttcggttga ccacggaatt ccagccacgc tgcagcagcg gctgtccggc
     8761 gtagagaacg gcaaccgatg ccagaaacag ctgcagcacc ggtacaattt caagcatgcc
     8821 aaaaaccatg accggtacgc tcacgaccgc ggcaatccag aatcgctggg tcatgtcgag
     8881 cagttccgga ttcggaccct catcggcttt cgcaatttcg ggttccagcg ccatgccgca
     8941 cttcggacat ataccaggcc ccatctggcg aacctcagga tgcatgggac agacatagat
     9001 ggtatcggat ttgggcggtt caggtttcga gccgggactc aggaacgact cgggatccga
     9061 ctgaaatcgg tcgcggcagc gcgggttgca gaagtagtag gtctttccgt tgtagctgaa
     9121 ttgtgccgcc gcggtctctg gcaggacgcg catcttgcag acaggatcga tttcgaattg
     9181 gggaggagtg ggggagattg caccaatttg tatcaggtcg ggcggcttag tcacaaacca
     9241 agtatgcagg acaactgtag gcgcggtcta tgaccgcgcg cgatttctaa ttgaaataca
     9301 aattcagcgt ggagagcgtg cgcggtcata gaccgcgcct acagtcagag ccttcggatg
     9361 cattagatcg cgacgtttcc ggtaggcgcg aaagatggca tagccaatcg agcccagtat
     9421 caaatatgga gcaaccatca gaatgacgat cccatagcgg aagctgctgg caagcgcccg
     9481 gccttccggg gacgatgcca gggccgtgcc gcacatcacg cactgggccc aacccggcac
     9541 ttcgagcagg ccaatcactc caaccagcat cagcgcagaa cgaaacc
//
LOCUS       DATMZQ010000021         6931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1051967, whole genome shotgun sequence.
ACCESSION   DATMZQ010000021 DATMZQ010000000
VERSION     DATMZQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051967"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..715
                     /locus_tag="VN496_00705"
     CDS             17..715
                     /locus_tag="VN496_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HXF15488.1"
                     /translation="MIDLHYWPTPNGHKISIFLEETATAYRLVPVNISTGEQFRPEFL
                     AISPNNRMPAIVDHAPADGGPAVSVFESGAILVYLAEKTGQFLPQDLRGRFEVLQWLF
                     WQMGGLGPMAGQNHHFSQYAPDKLPYAIDRYVNETARLYAVLDKRLAGREFVAGKYSI
                     ADMAAYPWIVPHQRQQQDLHDFPNLKRWFELLQARPAVIRAYEKGKSINTKPTIDDES
                     RKILFGQNAATVRR"
     gene            939..1961
                     /locus_tag="VN496_00710"
     CDS             939..1961
                     /locus_tag="VN496_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF15489.1"
                     /translation="MRLIERDQSARTSNAAPVVSVVVPTCNRPWLLERCLDALAGQKI
                     RSGFFEIIVVDDRPSAGTRAVFRSKAATVIRAGNEIRYVASSGPHGPAAARNRGWTAA
                     RAEIIAFTDDDTVPDALWLVNGLNALDDSLAAVCGRIVMPIPPLPTDYERDASGLERA
                     EFVTANCFCRKAILERVGGFDERFELAWREDSDLQFRILETGARIGIAPDALVVHPVR
                     PARWGISLQQQKKVMFDVLLFKNHPRLYRTRIRKSPRWDYYAIVGLMLAAIATGLSGV
                     SPWLPAIFTGLWALLTARFCMLRLRGTSRTAAHIAEMVVTSVLIPPLAVFWRLAGMLK
                     FRVGFA"
     gene            1963..2988
                     /locus_tag="VN496_00715"
     CDS             1963..2988
                     /locus_tag="VN496_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXF15490.1"
                     /translation="MDGAAADHRTGIAELPQFVQIEPVGQCNLRCKMCPIQFRTDGTP
                     DHPPAFMEFPVFCRLIDQFPQLTQLHLQGMGEPLMHPRFFDMVRYAAQRGIEVSTNSN
                     LTLLSERRAVECVNSGLRRIHVSLDGASAQTYEFIRVRAKFERVLRNIRRLAQAKQLL
                     HSEQPEICLVAVAMRKNLHELPDLVRLAHELHIDVLSVQHLCHDFGEESLPTKYQPMR
                     AFVESETLLNEEPMRVAHFFESAAQLAVALDLKLRLPNLAPGPRPEGRTRCDWPRRGA
                     YLSFEGDAMPCCMVATPDRINFGNMAKSSVASVWNNREYRTFRTRLDSDTPPEVCRTC
                     ALYKGTF"
     gene            3000..4010
                     /locus_tag="VN496_00720"
     CDS             3000..4010
                     /locus_tag="VN496_00720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXF15491.1"
                     /translation="MAALNILTWHTHGSYLYYLTQVPHEFHVMVKPGRPAGYGGRGGH
                     LPWGSNVHDMPVDEAKHARFDCVLFQDDHHYFEDQFRYLTDAQRRLPRIYLEHDPPRE
                     SPTDTRHPVDDPEVLLVHVTAFNDLMWDSGRTPRRIVEHGVIDPGVCYTGTLGRGIVI
                     VNNLMHRGRRLGADIFQSVRTQVPLDLIGMETQESGGLGEVLHRDMPAFVSRYRFLFN
                     PIRYTSMGLAVIEAMMSGVPVVGLATTEMATAIEDGRSGFVDTDVRKLIAHMRTLIAD
                     PALAWRLGEGARTAALRRFGIQRFIDDWLKAFAWVTDKRAAKSAPLRLKNNAGREAAG
                     MN"
     gene            complement(4020..4685)
                     /locus_tag="VN496_00725"
     CDS             complement(4020..4685)
                     /locus_tag="VN496_00725"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HXF15492.1"
                     /translation="MKVPSKLLRNMIIGALLTGAWSLAPAADQQTQSQTGASTSQSKL
                     DHKDKKFIEKAAEDGMAEVELGRLAQTKASNDQVKQFAQQMQQDHSQANDELKRIAQA
                     KGVTIPASTDHSHMKKMEKLQKESGADFDKQYMADMVKEHEKDLKFFRDEAQNAKDPE
                     LKTFAQKTAQKINEHLNMARQIAGQVGAEGGKTGMRNEHQPNKMSHADAGKPDTSASS
                     SNK"
     gene            4984..5175
                     /locus_tag="VN496_00730"
     CDS             4984..5175
                     /locus_tag="VN496_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15493.1"
                     /translation="MKQSASTPAANTSQQAPGDEAAPGTPGTGENICPVCNGSGRIAS
                     GECQNCRGTGRIITGIGGA"
     gene            5189..5785
                     /locus_tag="VN496_00735"
     CDS             5189..5785
                     /locus_tag="VN496_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXF15494.1"
                     /translation="MELARDDDPVRSSTSWVQQTTAVGINLGCGPIAQSGWINCDTRD
                     MRGVDLRCDVVDGLAFVSDCADYIAAIHVLQDLAYAQIVPALREVRRVLKPGGVFRLA
                     VPDIDRAISAYARRDAAYFYVPDEHARSIGAKLVTQIIWYGSVRTPCNFEFLSESLSL
                     AGYRDIKRCAFGVTRSRFPALASLDNRERETLFVEALK"
     gene            5787..6491
                     /locus_tag="VN496_00740"
     CDS             5787..6491
                     /locus_tag="VN496_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXF15495.1"
                     /translation="MGVSAGPGKPLLVVSPHLDDGVLGCGELLAGEPGAVVLSLFAGP
                     GQNPDVCTEWDAACGFTSARQALAVRAIEDDTALGLLQAMPSRLDFPDDQYREVGDRV
                     SVEGLAGAIRQAVNHYQPRSVAIPFGLFHRDHALAHEASLRVAREIQECAWLAYEDAF
                     YRRIPGLLQQRLCALASMGWVATPMPAPEQRRSTAKQRAVQCYASQLRGLESPGRPGR
                     LDAFAPEGYWRLKAPT"
     gene            6510..>6931
                     /locus_tag="VN496_00745"
     CDS             6510..>6931
                     /locus_tag="VN496_00745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF15496.1"
                     /translation="MAAGSNPSAVTVVILTYNRAEELLRTVLHTMSLPEQPPIIVVDN
                     GSSDYSARLVVERFPSVQVISMPANVGAAARNIGALAARTKYVAFSDDDTVWAPGSLA
                     TASRLLQDHPGIGVLSARILVGAQGREDPACRAMAGSPL"
BASE COUNT         1365 a         1987 c         2059 g         1520 t
ORIGIN      
        1 ccctgaaatc gccaccatga tcgatctgca ttattggccc actccaaatg gacacaaaat
       61 cagcattttc ctggaagaaa ctgctaccgc ataccggctc gtgccggtga acatcagcac
      121 cggcgagcag tttcgtccgg agtttctggc gatttctccc aacaatcgca tgccggcgat
      181 agtcgatcat gcgccggcgg atggcgggcc agcggtttcc gtgttcgaat ccggcgccat
      241 tctggtctac ctcgccgaaa aaaccggcca gtttcttcct caagatctgc gcggccgctt
      301 tgaagtgttg cagtggttgt tctggcagat gggtggactg gggccgatgg ccggtcaaaa
      361 tcatcatttc agccagtacg cgccggataa attgccgtac gcaatcgacc gttacgtcaa
      421 cgagaccgcg cggctttacg cagtgctgga caagcggctt gccggtcgcg aattcgtagc
      481 cggaaagtat tccattgccg atatggcagc gtatccctgg atcgtgccgc atcaacggca
      541 acagcaggac ttgcacgatt ttccaaatct gaagcgatgg ttcgaactcc tgcaggcgcg
      601 acccgcggtg attcgagcct atgaaaaagg caagtccatc aataccaagc ctactatcga
      661 cgatgagtcg cgcaaaatcc tgttcggaca aaacgccgcg acagtacgcc gataaggctg
      721 cggtacggaa acgaaggaga aatgcgacag cactgcggcg cacgcaagct ttaccggtac
      781 gcaggtaaaa ctttcttctg gaataacgaa cacgcgtgcg gaggcgagcg cccacgaggg
      841 cgcagtgtat tggaacggta gttgcttttc agtttgtgaa ccgggttcac aaaaaattta
      901 ctaacgtgcc gaccagttcg ccgctagcgc acacatcaat gcgcttgata gaaagagatc
      961 agagcgcacg cacctctaac gctgcacccg ttgtcagtgt ggtagtaccg acatgcaacc
     1021 gcccctggtt attggagcgc tgccttgatg cgctggccgg ccagaaaatc cgttccggct
     1081 tcttcgaaat cattgtagtc gatgatcgtc cgagcgcggg cacgcgagcg gtttttcgaa
     1141 gcaaagcagc gaccgtgatt cgcgccggaa acgaaatacg gtatgtcgca tcttccggcc
     1201 cgcatggccc cgcggctgca cgcaatcgcg gctggacagc agcgcgcgcc gagattattg
     1261 cattcaccga cgacgacacc gttccagacg ccttgtggct ggttaatgga ttgaacgctt
     1321 tggacgattc attagccgcg gtttgcgggc gcatcgtgat gcccattccg ccgctgccaa
     1381 ccgactatga gcgcgacgcg agcggattgg agcgggcgga atttgtcacc gcaaactgtt
     1441 tctgccgcaa agcgattctt gaacgcgtgg gcggtttcga cgagcgcttc gagttggcgt
     1501 ggcgtgaaga ctcggacttg caattcagaa ttcttgaaac tggggcgcgc attggcattg
     1561 ccccagacgc cctggttgtt catcccgtgc gccctgcgcg gtggggtata agtcttcagc
     1621 agcagaagaa agtcatgttc gatgttttgt tgttcaagaa ccatccgcgt ctctatcgca
     1681 cccgcatccg caagtcgcca cgctgggact actacgccat tgtggggctg atgctcgccg
     1741 ccatcgcgac tggtttgtct ggggtaagcc catggttgcc tgcgatattc accggactat
     1801 gggcactgct caccgcgcgt ttttgcatgc ttcggctgcg cgggacttcg cgcacggcag
     1861 cgcacatcgc cgagatggtc gtcacgtccg tgctaatccc gccgttggcg gtattctggc
     1921 gcctggccgg catgttgaaa tttcgcgttg ggtttgcctg atatggacgg cgcagcggcc
     1981 gaccaccgca cgggtatcgc cgaactgccc cagttcgtgc aaatcgagcc ggtagggcaa
     2041 tgtaatttgc gctgcaagat gtgtcccatc cagtttcgca cggacggcac gccggaccac
     2101 ccgcccgcat tcatggaatt cccggtgttt tgccggctta tcgaccagtt cccgcagctc
     2161 acgcaattgc atctgcaggg catgggagaa ccactgatgc atccgcgctt tttcgatatg
     2221 gttcgctatg ctgcgcagcg tggcatcgag gtttccacga acagcaatct cacgctgctc
     2281 agcgagcgcc gcgcggtgga atgcgtcaac agcggactac gccgtattca cgtgtcgctc
     2341 gatggcgcaa gcgcccagac ctacgagttc attcgggtgc gcgccaaatt tgagcgtgtg
     2401 ctgcgcaata tccggcgcct tgcgcaagca aagcaactcc tgcacagcga gcagccggag
     2461 atatgcctgg ttgccgttgc gatgcgcaaa aacctgcacg agttgcccga cctggtgcgg
     2521 cttgcgcatg agttgcatat cgacgtgctg agtgtgcagc acctgtgtca cgattttggt
     2581 gaagagagcc tgccgacgaa gtaccagcca atgcgggcgt ttgtggaaag cgaaaccctg
     2641 ctgaacgaag aacccatgcg agtcgcgcac tttttcgagt cagcagcgca gcttgccgta
     2701 gcgctggact tgaaattgcg tctgccgaat cttgcgccgg ggccccggcc ggaagggcgc
     2761 actcggtgcg attggcctcg gcgcggcgct tacttgagtt tcgagggaga tgccatgccg
     2821 tgttgcatgg tcgcgacgcc cgatcgcatt aattttggca acatggcgaa gtcgtccgtg
     2881 gcctcggtat ggaataaccg cgaataccgt acctttcgta cccgactgga ctccgatact
     2941 ccgcccgagg tttgccgtac ctgcgcactg tataagggaa ctttttaggt gcccaaacca
     3001 tggccgcact taatatcctg acctggcaca cacacggcag ttacctgtat tacctcacgc
     3061 aagtcccgca tgaattccat gtcatggtca agccaggacg gcctgccggc tatggcggac
     3121 gcggcggaca cttgccgtgg ggcagcaacg tgcacgacat gccggtcgat gaagcaaagc
     3181 acgcccgctt cgattgtgtg ctgtttcagg acgatcatca ttatttcgag gaccagtttc
     3241 gctatttgac ggacgcacag cgccgcctgc cgcgcattta tctcgagcac gacccgccgc
     3301 gcgaatctcc gaccgatacg cggcacccgg tggacgatcc tgaagtgttg ctggtgcatg
     3361 taacagcatt taatgacctc atgtgggaca gcggccgaac gccgcgacgg atcgttgagc
     3421 acggtgtgat cgatcccggc gtctgctaca cgggaacact cgggcgcggc atcgtgattg
     3481 tgaacaacct gatgcaccgt ggccgaaggc tgggcgccga tattttccag agcgtacgca
     3541 cgcaggtgcc gctggatctg atcggcatgg aaacacagga gtcgggcggg ctcggcgaag
     3601 ttctgcaccg cgatatgcct gcctttgttt cgcgctaccg tttcctgttc aatccgatcc
     3661 gctataccag catgggtctc gccgtcatcg aagccatgat gtctggcgtg ccagtggtgg
     3721 gactcgccac caccgagatg gcgactgcga ttgaagacgg gcgctccgga tttgtcgata
     3781 ccgacgtgcg aaagctcata gcccatatgc gcacattaat cgccgaccct gctctggcgt
     3841 ggcggttggg cgaaggcgcg cgcacagccg ccctgcgacg tttcggtatc caacgtttta
     3901 tcgatgattg gctaaaggcg ttcgcctggg tgacggacaa gcgggccgca aagtctgcgc
     3961 ccctgcgctt gaaaaacaat gccggccgag aggcggccgg catgaattga cgtactgagc
     4021 tatttgttgc tgctgcttgc cgacgtatcg ggtttgccgg catctgcgtg ggacatcttg
     4081 ttcggctggt gctcgttcct cattccggtt ttgccgcctt cagctccgac ctgtccggcg
     4141 atctgccggg ccatattcag gtgctcattg attttttgcg cggtcttctg ggcaaaggtc
     4201 ttgagctcgg gatctttggc attttgagct tcgtcccgga agaatttcag atctttttcg
     4261 tgttccttca ccatatcggc catgtactgc ttgtcgaaat cagcaccgga ttctttttgc
     4321 aatttttcca ttttcttcat gtgcgagtga tccgttgacg ccggaatcgt cacgcccttg
     4381 gcctgcgcaa tccgtttcag ttcatcgttg gcctggctat ggtcttgctg catctgctgc
     4441 gcaaactgct tgacctggtc gttggatgct tttgtctgcg caagacggcc cagttcaact
     4501 tcggccatgc cgtcttcggc tgccttttcg atgaacttct tgtctttgtg gtcaagcttc
     4561 gactgactcg tgcttgcccc ggtttggctt tgagtttgct ggtccgcggc tggagcgagt
     4621 gaccatgcac cggtcagcaa cgcgccgatg atcatatttc gaagtagttt agagggtact
     4681 ttcataaggg tctcctgaac attctgttcg atagagttca gcatgcaccc cccatggatg
     4741 cacggaacgc gtccgtgcgt cgttgcaagg accataccca aatcgcaagc gcccgattac
     4801 gcggcattag atggcgcggc acggcggacc gctgtaaaaa agactaaacg ttcagcaaat
     4861 tttgctgacc aacagcaagg agttgcgggc ctgcgaaacc aaaaaaagac gacccccctt
     4921 gagcgcggat ccacttacgg caccatattt gctgaaaacg taatacctac cctggagtgt
     4981 caaatgaagc aatcagcgag cacaccggca gccaacacat cacagcaagc gcccggagac
     5041 gaagcggccc ccggaacacc aggcactggc gaaaacatct gtccggtatg caatggcagt
     5101 gggcgtatcg cttccggtga gtgtcagaat tgcaggggca cgggacgcat cattaccggc
     5161 atcggcggcg cctgaagttt gcccggagat ggaacttgca cgggacgatg acccagtacg
     5221 ttccagtacg tcgtgggttc agcagaccac tgcggttggc atcaatttag gctgcggacc
     5281 gattgcgcaa tcgggctgga taaactgtga tacacgcgat atgcgcggag tggatttgcg
     5341 ctgcgatgtt gtcgatggac tggcattcgt ctcggactgc gccgattaca tagccgcaat
     5401 tcacgtgttg caggatctgg cttatgcgca aatagtgccc gccttgcgcg aagtccggcg
     5461 cgtactgaaa cccggaggtg tctttcgact cgccgttccg gatatcgacc gcgccatcag
     5521 cgcgtatgcc cgccgggatg cggcgtattt ctatgtgccc gacgaacatg cacgcagtat
     5581 cggcgcgaaa ctggttactc aaataatctg gtatgggtcg gtgcgtaccc catgcaactt
     5641 tgaatttctc agcgagtcgc tctcccttgc cggctatcgc gatatcaaac gctgtgcatt
     5701 cggcgtcacg cgcagccgat ttccggcgct ggcgagtctg gataatcgtg aacgggaaac
     5761 attgtttgtc gaggcgctga aatagcatgg gcgtaagcgc cggcccaggt aagccgctgc
     5821 tggtggtctc gccgcacctc gatgatggag tactgggctg cggtgaattg ctggccgggg
     5881 aacccggtgc ggttgtgctc tcgctgttcg ccggccccgg ccaaaatccc gacgtgtgca
     5941 ccgagtggga cgccgcgtgc ggatttacct cggcgcggca ggcgctggcc gtgcgtgcga
     6001 tagaagacga taccgcgctc ggtctgttgc aagcgatgcc atcccgcctg gactttccgg
     6061 atgatcagta tcgtgaggta ggcgaccgag taagtgtgga aggactcgct ggcgcgattc
     6121 gacaagcagt aaatcactat caaccgcgga gcgtggccat cccgtttggc ttgtttcacc
     6181 gggatcatgc gctggcgcat gaggcttcac tgcgcgttgc gcgcgaaatc caggagtgtg
     6241 cctggcttgc ttacgaggat gcgttctatc gccgcattcc gggattgctg cagcagcgat
     6301 tgtgtgcact ggccagcatg ggctgggttg ccacgcccat gccggcgccc gaacagcgca
     6361 gaagtactgc caagcaacgt gcggtgcagt gctacgcaag tcaattgcgc ggtctggaat
     6421 cgccgggccg gccgggtcgc ctggacgcat tcgcccccga gggctattgg cgcctgaagg
     6481 cgccaacctg actgacaggg agagttcact tggcagccgg atcgaatccc agtgcggtaa
     6541 cggtggttat cctgacttat aaccgcgcgg aggagttgct gcgcaccgtc ttgcatacca
     6601 tgagtctgcc agagcagcca cccatcatcg tcgtagacaa tggatcgagc gactactcgg
     6661 cgcgcctcgt ggtggagcgg tttccttcag tgcaggtcat ttcgatgccg gcaaatgttg
     6721 gcgctgccgc gcgcaacatc ggcgcccttg cagctcgcac gaaatacgta gcgttctcgg
     6781 acgatgacac ggtatgggcg ccgggttcgc tggctactgc cagcaggctg ttgcaggatc
     6841 atcccggcat cggcgttctg tcggcgcgca ttctggtggg agcgcaaggc cgcgaagacc
     6901 cggcgtgccg cgccatggca ggcagcccgc t
//
LOCUS       DATMZQ010000022         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_699653, whole genome shotgun sequence.
ACCESSION   DATMZQ010000022 DATMZQ010000000
VERSION     DATMZQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699653"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VN496_00750"
     CDS             complement(<1..246)
                     /locus_tag="VN496_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15497.1"
                     /translation="MGRYRDGGRQLLRRGSLASLFALLSGCSWLWPAPEPEPEPVQYS
                     DAALAREQVTILPKEDGSIGGVVVRHYGVEVLLDKPYA"
     gene            451..840
                     /gene="msrB"
                     /locus_tag="VN496_00755"
     CDS             451..840
                     /gene="msrB"
                     /locus_tag="VN496_00755"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXF15498.1"
                     /translation="MGHLEKPETQWQSDLTPEQYEVLRKEGTERRFTSPLNAEYRKGV
                     FVCAGCGAQLFKSDMKYDSKTGWPSFFTAIPGALETRTDKKLFVERTEYHCVQCGGHQ
                     GHVFEDGPNPTGLRYCNNGVALKFIPE"
     gene            complement(858..1316)
                     /locus_tag="VN496_00760"
     CDS             complement(858..1316)
                     /locus_tag="VN496_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HXF15499.1"
                     /translation="MANNRSYESVILPAWLRWSLYLATIALFATGGIWLIAHYGMGPA
                     SGDELPHPAEPWILRVHGFAVMVGLFIYGSLLRAHMLKAWTLRRNRITGACVATVLVL
                     LTVTGYMLYYVGGETSRPIISVAHWIIGLAIGALLPLHIWRGRRSQRAVR"
     gene            complement(1321..2016)
                     /locus_tag="VN496_00765"
     CDS             complement(1321..2016)
                     /locus_tag="VN496_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HXF15500.1"
                     /translation="MVKRHWERHRTERIGWLRASVLGANDGIVSTASLVLGVAAAHAT
                     HGAIMVAGVAGLVAGAMSMAAGEYVSVHSQADTERADLDRERKELQTDDVGERRELAA
                     IYVGRGLDQGLANKVADQLMAHDALGAHARDELGIVHSNIARPIQAAFASAGSFSVGA
                     AMPLIVTAIAPQSLLIPVVSGASLIFLAALGALAARAGGAKMSVGSLRVAFWGALAMV
                     TTAGVGALFGAAV"
BASE COUNT          499 a          709 c          618 g          397 t
ORIGIN      
        1 cgcgtagggc ttgtcaagca gcacctcgac gccgtagtgc cgcaccacca cgccgccgat
       61 tgacccatct tccttcggga ggatagtgac ctgttcacgc gcaagagccg catcgctgta
      121 ctgcaccggc tccggctcag gctctggcgc gggccataac caggaacatc cggacaacag
      181 cgcaaacagc gaagcaagcg aaccgcggcg cagcaattgc cgtccgccgt ctctgtaacg
      241 acccataaaa ccagggattc ttccggactg ccatgacgac gccacggtgc ctctccttgc
      301 tgttatcctc gttttggcca gtagccgatt tttatcacgg tgccgcagca ccccggccgg
      361 gcgagcttgt cacagataac ataacacccg acgcgcagca aatgcgaatt caaatgagcg
      421 gcgccttggg gtgcgcaagg agtatctaac atgggacacc tggaaaaacc ggaaacacaa
      481 tggcagtccg atctgacgcc agagcaatat gaggtactgc gcaaggaagg caccgaacgc
      541 cgcttcacca gtccactcaa tgcggaatat cgcaaaggcg ttttcgtatg cgcgggctgc
      601 ggcgcgcagt tgttcaaatc ggacatgaaa tacgacagca aaacgggatg gccgagtttc
      661 tttaccgcca ttcccggcgc cctggaaacc agaacagaca aaaagctgtt tgtcgagcgc
      721 accgaatatc actgcgtgca atgcggcggc catcaaggac acgtgttcga ggatggtccg
      781 aacccgaccg gtctgcgcta ctgcaataac ggcgtcgcgc tgaaatttat cccggaatga
      841 ccgggaaagc gagaccttca gcgcacagcc cgctggctgc ggcggccccg ccagatatgc
      901 agcggcagaa gtgcgccgat ggcgagtcca atgatccagt gcgcgacact gatgatcggc
      961 cgggatgttt cgcccccaac gtaatacagc atgtagccgg ttaccgttag caataccagc
     1021 accgtcgcaa cgcacgcccc tgtaatacgg ttcctcctca gcgtccaggc tttcagcatg
     1081 tgcgctctca gcaaggatcc ataaataaac aggccaacca ttacggcgaa accatgcact
     1141 cgcagaatcc agggctctgc gggatgcggc agttcgtcac cgctcgcagg gcccatgccg
     1201 taatgtgcaa tcaaccagat gcccccggtc gcgaatagag cgatggttgc gaggtataac
     1261 gaccatcgca gccatgccgg cagaatcacc gattcgtacg acctgttatt ggccatgtgc
     1321 ttaaactgca gctccgaaca gagcaccgac tccggcggtc gttaccatgg cgagtgcgcc
     1381 ccaaaaagca acccgcaagg aacccacgct cattttggcg ccaccagcgc gcgctgccag
     1441 ggcaccgagc gctgccagaa aaataagcga cgccccggaa accaccggga tgagcaaaga
     1501 ttgcggtgct atggccgtga cgatcaacgg cattgccgca ccgaccgaga aacttccggc
     1561 cgaagcgaat gccgcctgga ttggccgcgc gatattgctg tggacaatac cgagttcatc
     1621 gcgggcatgt gcgccgagtg catcgtgcgc catgagctgg tcggcaacct tgttcgccag
     1681 tccctgatcg agtccgcgtc ccacgtagat cgcagccaat tcccggcgct ccccgacatc
     1741 atcggtctgg agttccttgc gctcgcgatc gaggtccgcc cgttcggtat cggcctggga
     1801 atgcacggac acgtattcac ccgccgccat cgacatcgcg cctgcaacca ggccggccac
     1861 ccccgccacc atgatggcgc catgggtcgc atgcgctgcg gccacgccga gcaccagact
     1921 cgctgtcgaa acgatgccgt cgttcgcacc cagcacagac gcgcgcaacc agccgatgcg
     1981 ttcggtgcga tgtcgttccc aatggcgttt gaccatgtgt gctgtccagt gattgaaaga
     2041 tatgccgtgc aactattcgg gattcatacc gcagcggccg ttcatgatgc cgtggctcgc
     2101 ggcagcatgt tggccagcat tgccatcatc atatcaatct cctctttcga tacggtcaat
     2161 gacggcatga accgcaacag gtttggacgc ggcgcattca acagcaggcc cgcgtctcgt
     2221 gcc
//
LOCUS       DATMZQ010000023         3337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3461615, whole genome shotgun sequence.
ACCESSION   DATMZQ010000023 DATMZQ010000000
VERSION     DATMZQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461615"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VN496_00770"
     CDS             <1..319
                     /locus_tag="VN496_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF15501.1"
                     /translation="NPNTVQALTNAMVRALKWLKAATPDEVAMQVPPEYLLGDRMLYL
                     EAYRKNSESYSKDGLFPPPGAEALLRVLSGFEPAVRDAPGINSADAYTNEFVSAALTK
                     FRN"
     gene            501..878
                     /locus_tag="VN496_00775"
     CDS             501..878
                     /locus_tag="VN496_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF15502.1"
                     /translation="MSFPVAIMFMFLTVVPIIAEAQTDPVTGKEIFEQMCAGCHGTYG
                     NGQEGTKAGFVPRIGTLANKEYMDSVPDDYLKMIIKKGGAYMGKIAAMPAWEHKFNDE
                     QIESLVAHIRTFTGTADKKADKK"
     gene            1167..2651
                     /locus_tag="VN496_00780"
     CDS             1167..2651
                     /locus_tag="VN496_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15503.1"
                     /translation="MSVFAAGLDTITSITVDKQDNVWVAISGNTFGFPPEGIDKPHVK
                     IYDKSGKLIKDRVGLGMFKSFALNEIGYCPENGRTYVGDYSYGIWEIDGVNGTPKLIM
                     NEVPIGDHALGGITCRDGWLYYAVGAPTNSGFSDPDIHGWTDAVDPYWEKRTTDGMPA
                     LPRDPPCRDITLTGLNIRDSQGNLTGAYLPKGTASKPGQVIKAQKPCGGAIHRAKLKA
                     DSSYTHDDWEVYAMGLRNSSGVAFGPKGSRFEKALAVSDNGHNDKGNRRVANAAERLF
                     IFTEKGQDAGFPDKDGINFVNIKRSGPDVYRGNKFDPTRPNPQLYIGNKPFIPTLPPY
                     RFIDHSIGVRGTPLIIANPNPNGYVNPIMEWDTNNPMDGLAWAPKAFDSGGDVIYTAV
                     FGIIDNGPESLRPMWPAIVRVELLNPAGVKWSIFAENIDPGPNAYQKKENRGGFERTN
                     DVEFSTDGKTMYVADYGELYVNYQMESPFYTTPKSAVVWAITKQ"
     gene            complement(2748..3203)
                     /locus_tag="VN496_00785"
     CDS             complement(2748..3203)
                     /locus_tag="VN496_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15504.1"
                     /translation="MFNRARVLPALLAAVSASLVLPCMPASAQADQASTRDQWSAPVE
                     AQFRGCESAGWCRFWIESVDPLAQSFYRVRPNGAPRVTAEEPVATALRDRLNVLLSDM
                     IHQAKHIVLHDLHQLDDGTFTATVTVNGIDLATDPTLVDLNRKLHATAR"
BASE COUNT          761 a          944 c          998 g          634 t
ORIGIN      
        1 caatccgaat acggtgcagg cgctgacgaa cgcgatggtg cgcgccctga aatggctgaa
       61 ggctgcgacg ccggacgaag tagccatgca ggtgccgccg gaatatttgc tgggcgatcg
      121 catgctttac ctggaagcct atcgcaagaa cagtgaaagc tactccaagg acggcctgtt
      181 tccgccgccg ggcgctgaag cactgttgag ggtgctgtcc ggtttcgagc cggcggtgag
      241 ggatgcaccg ggcataaatt cggcggacgc ttacacgaac gaattcgtga gcgctgcact
      301 gaccaaattc aggaattgag cggggcccgg ccacccgaat atgttgcgta gcaaccagcc
      361 tctacggcgg atgcggggtt gcggagataa agccacgcgg gcccggctcg aaaaacgctc
      421 tcttgagcat cgccaagaca cgtgatacgg tgccattcct atccgctgac cctatttacc
      481 tcgcgacgcc gaggacgcgc atgagcttcc cagttgcaat catgtttatg tttttgactg
      541 tcgtcccgat aatagccgag gcacagaccg atcccgtcac gggcaaggaa atattcgagc
      601 agatgtgcgc cggatgccac ggtacctacg gtaacggcca ggaagggacc aaagcggggt
      661 tcgtgcctcg tatcggcacg cttgccaaca aggaatatat ggattcagtg ccggacgact
      721 atctcaaaat gatcatcaag aagggcgggg cgtacatggg caagatcgcg gccatgcctg
      781 cctgggagca taaatttaac gacgaacaga tcgagagcct cgtcgcccat atccgcacat
      841 tcacgggaac ggcggacaag aaagcggaca agaagtaggg ataacaaaga tccggtcggt
      901 ccaacggcca ggtatgcagg tacgtcgtaa cgcagttgaa gtggaactct gaccaagcaa
      961 gtgcagggga attctaacca ggaggagaag aaatgaaaat tctaaagtat gcggcaatac
     1021 tcgcattggc cgcggggggc ttgtccgggt ccgcctttgc gcaagaccgc gcaaaggccg
     1081 agccaggcgg ccctgcggga aaatggtcca cgcgcacgcc cgttacgccg gatccgagca
     1141 agatcaaggt tccggcgggc tacaaggtga gcgtgtttgc tgccggcctc gataccatca
     1201 catccatcac ggtcgacaag caggacaatg tctgggtcgc catttcgggc aacaccttcg
     1261 gctttccgcc ggaaggtatc gacaagcccc atgtgaagat ctacgacaag tccggcaagc
     1321 tgatcaagga ccgcgtcggg ctgggcatgt tcaaatcgtt tgctttaaac gaaattggct
     1381 attgcccgga aaacggtcgc acttacgtgg gtgactacag ttacggcatc tgggagatcg
     1441 atggcgtcaa cggcaccccg aagctgatca tgaacgaggt accgatcggc gatcacgcgc
     1501 tgggtggcat cacttgccgt gacggctggt tgtattacgc cgtcggcgcc cccaccaact
     1561 ccggcttttc cgatccggac atccatggtt ggaccgatgc ggttgacccg tactgggaaa
     1621 agcgcacgac cgacggcatg ccggcgctgc cgcgcgaccc accctgccgt gacatcacac
     1681 tgacaggact gaacatccgc gatagccagg gcaatctcac gggcgcctac ctgccgaagg
     1741 gtactgcgtc caagccgggc caggtcatca aagcgcaaaa accctgcggt ggcgccattc
     1801 accgggcgaa gctgaaggcc gacagcagct acacccacga tgactgggaa gtctatgcga
     1861 tggggctgcg taattcttcg ggggtagcat tcggtccgaa gggctcgcgg ttcgagaaag
     1921 ccctggccgt cagtgacaat gggcataacg acaagggtaa tcgccgggtt gctaatgcgg
     1981 cggagagact cttcatcttt actgaaaaag gccaggatgc gggattcccg gacaaggatg
     2041 gcattaattt cgtcaacatc aaacgttccg gccccgacgt ttatcgcggc aacaagttcg
     2101 acccgacgcg gccgaacccc cagttgtaca tcggcaacaa gccgttcatt cccaccttgc
     2161 cgccgtaccg tttcatcgac cattcgatcg gtgtgcgcgg cacgccactg atcattgcca
     2221 atccaaatcc aaacggctac gtcaacccga tcatggagtg ggacaccaac aaccccatgg
     2281 acggcttggc ctgggcaccg aaggcattcg attcaggcgg cgacgtaatc tacaccgcgg
     2341 tgttcggcat catcgacaac gggccggaaa gcctgcgccc gatgtggccg gccatcgtcc
     2401 gcgtcgagct gctgaatccg gccggcgtga agtggtcaat cttcgcggaa aacatcgacc
     2461 cgggtcccaa cgcttaccag aagaaggaaa accggggcgg cttcgagcgc accaacgatg
     2521 tcgagttcag cacggacggc aagacgatgt acgtcgccga ctacggcgaa ctctatgtga
     2581 actaccagat ggaatcgccg ttctacacga cgccgaaatc tgccgttgtc tgggcgatca
     2641 cgaagcagta aattgcgcgt tttctgaaat cccgggggct tcggcctccg ggattttttt
     2701 cgtccggttg cggcttgcgt ttggaaagat ttgttaccgg attgcggtta acgcgccgta
     2761 gcgtgcagct tgcggttcaa atcgaccagt gttgggtctg ttgccagatc gatcccgttc
     2821 accgtcacgg tcgcggtgaa tgttccatcg tccagctgat gcagatcatg cagaacgatg
     2881 tgctttgcct gatggatcat atccgagagc aggacattga ggcgatcgcg caacgcggtt
     2941 gcaacgggct cttcagcggt aacccgagga gcgccattag ggcgaacgcg gtaaaaagac
     3001 tgagccaggg gatccacgga ttcaatccag aatcggcacc acccagcgga ctcacagccg
     3061 cgaaattgtg cctctaccgg cgcggaccat tggtcgcggg tagaagcctg gtctgcctgt
     3121 gccgatgccg gcatgcacgg caggacaaga gatgcgctga ctgccgcaag caaggccggt
     3181 aatacgcgtg ctcgattaaa catgcccggt gttatatcag ttttcgttgt tgcggccgac
     3241 atctgaaaat gtttctggcc gcttgaacag cgcagcgggc aggcccggta gaatggcggt
     3301 tcgcacgtcc gcttccttgg aagcctccct tgttttg
//
LOCUS       DATMZQ010000024         7991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3124683, whole genome shotgun sequence.
ACCESSION   DATMZQ010000024 DATMZQ010000000
VERSION     DATMZQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3124683"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN496_00790"
     CDS             <1..343
                     /locus_tag="VN496_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HXF15505.1"
                     /translation="AWRVRFALQNTGWLPSYVTKMAKNRKLARGVVCEIELPKEARLE
                     TGKQREELGQLEGRAYKSAAANTWAGSSADETDERVSVEWVVCAPIGTSIKLLARHER
                     AGVATTRVTLE"
     gene            354..863
                     /locus_tag="VN496_00795"
     CDS             354..863
                     /locus_tag="VN496_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15506.1"
                     /translation="MTTSRATINTIGAAFFATALPPLAIALGWLIVPSESLPVPLWTM
                     GCGGAALAFAGLALLMPEYLPGPRGILAALSITALAGIFNWIAFGHFQGGWRFMLESL
                     MNSLALRHNAAHNVVVLFAVLLNLLALLGWTQWFREMTRRDGTPLKSLGRKARSKRKP
                     ADTGASAQD"
     gene            892..1203
                     /locus_tag="VN496_00800"
     CDS             892..1203
                     /locus_tag="VN496_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15507.1"
                     /translation="MRRIALIVMFMVVAAYSTTPSHAEDGNKQHSTDRFKSDVKAVGK
                     GAKDAAVDVGHQIATGTKKAYKSAKDKIKKDAKDGKPGDGSLASKNDALPAVTSGRKP
                     N"
     gene            complement(1235..1705)
                     /locus_tag="VN496_00805"
     CDS             complement(1235..1705)
                     /locus_tag="VN496_00805"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HXF15508.1"
                     /translation="MRLARTLRAVAIYEAFKGVLVLLIGIGLVLGLHRYIEYVGGQII
                     AVLHINPASRFARMLLSVTSHVTYGQLWAFAGFAAVYAGLRFLMAYGLWFERRWGEWL
                     VACSAAIYLPLEIFELFNEANWVIVVVIVVNVLIIALMAAALHYTRPAVPADVP"
     gene            complement(1913..2935)
                     /locus_tag="VN496_00810"
     CDS             complement(1913..2935)
                     /locus_tag="VN496_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXF15509.1"
                     /translation="MNAPLRVGIAGIGRMGQRHAFNLAHRTLRAELVAACSPIAGELE
                     WARDKLGVKHLYGDYKDLLAHPELDAVFLVTPTTLHADQIVQGLHAGKHVFSEKPLAL
                     NIPDCLKVEEEAARYSEQKVMIGFVRRFDPSYCDAYAKVKAGLIGRPFLVRSQTADQN
                     DPSGFFVKFAPTSGGAMLDMCVHDIDVARWFLGAGAAVRVYAAGTIAIHQGLAQCGDV
                     DNGVAICEFRDGQIACFYASRTMAHGHETLSEIVGTRGRLMVGQNPRIDRVEISDAQG
                     VRNECTPTFYERFAEAFQLEAQAFVDAVLDDRPVPVTLRDATEATRIGIALTQSLGSG
                     RAVDVE"
     gene            complement(3109..4095)
                     /gene="iolG"
                     /locus_tag="VN496_00815"
     CDS             complement(3109..4095)
                     /gene="iolG"
                     /locus_tag="VN496_00815"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HXF15510.1"
                     /translation="MIEVALFGAGRIGKIHGANLVAQSGVRLKYVIDINGAAAGELAQ
                     RHAARVADSDVAFKDPAIKAVVIASSTDTHAALIDRAAAAGKAIFCEKPVDLALERAL
                     ACAASVSKAGVLCMIGFQRRFDPTFAALKQRLDAGEIGNPEMLIITSRDPGPPPISYI
                     KVSGGIFRDMLIHDFDLFRWILGDEAQSVHASASCLVQKEIGEAGDVDSTAVTIRTRA
                     GRLCQINTSRRAAYGYDQRFEVLGPKGQLQAGNHRPTQVFAHTASSVSADKPEHFFLE
                     RYRTAYALEIAHFFDALAQGTPVKTTLQDGVRALELADAATRSWQEGRVVTL"
     gene            4256..6127
                     /gene="iolD"
                     /locus_tag="VN496_00820"
     CDS             4256..6127
                     /gene="iolD"
                     /locus_tag="VN496_00820"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HXF15511.1"
                     /translation="MGLTEAGMGGKIRLTMAQALVRYLARLRTDLPGDVPLFGGVFAI
                     FGHGNVAGIGEALYQVRDQLPTYRSHNEQAMTLSAVAFAKSHMRRRMMACTTSIGPGA
                     TNMVTAAAVAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDLHDGGISANDCLKPVSRYF
                     DRIVHPQQLLTALPRAIHVLTDPALCGPVTLALPQDVQTMAYDWPEEFFSPHRITLRM
                     LSPAPSELREAAATLKQAKRPLLVAGGGILYGDATGVLRDFAEAHGVPVAETQAGKGA
                     LPWDHALQLGSLGVTGSTAANAIARDADLVFAVGTRLQDFTTGSHSLFTQAKLLNLNV
                     NGFDATKLGGLPLQADARSGLQALDMALPGWKADARWTAQAAQLATQWRTTVDSITGN
                     RDVKLPYEGEVIGAVQRSSPNSAERDIVVCAAGGLPGELHKLWRTSLPGGYHLEYGYS
                     CMGYEIAGGLGVKMARPDREVIVIVGDGSYLMMNSEIATSIMLGLKLVIVLLDNRGYG
                     CIHRLQEACGGAPFNNLFDDGVATANGAPRIDFVAHARAMGANAEMADDIGALEAALR
                     RARAANRTSLVCIATDPKRTTLEGGWWWEVAVPEVSSRSEVTVARKSYEQGKQQQKP"
     gene            6124..7032
                     /gene="iolE"
                     /locus_tag="VN496_00825"
     CDS             6124..7032
                     /gene="iolE"
                     /locus_tag="VN496_00825"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HXF15512.1"
                     /translation="MSRNSFDVKIGINPISWANDDLPELGGDTPLERILSEGSAIGYR
                     GFELGNKFPREPEMLRKVLAQYGVELVSGWYSGQLARRSVDDEIAAASSHVQLLAKNG
                     AKVMVYGEVADSIQGKQDMPLSRRPQFTGDTQWRDYAGRLDAFAHYTSSRGIKLAYHH
                     HMGAYVQSGADIDRLMSLTGSQVGLLLDSGHLSFAGSDPLAVLNKHIARVCHVHCKDV
                     RPSVLGQAYKEDWSFLQAVLKGVFTVPGDGAIDFRSLLVRLQQHGYRGWLVVEAEQDP
                     ALAPSYQYAQMGFRHLSALVNSYVAG"
     gene            complement(7090..>7991)
                     /locus_tag="VN496_00830"
     CDS             complement(7090..>7991)
                     /locus_tag="VN496_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HXF15513.1"
                     /translation="MNNPFFDQARDGCKKAEKESAGKIECRYIGPGEHGGGDEQVKVV
                     QDLITAKVNGIAVSPSNAPAMAKVLERAKAAGIPVTTWDSDLLPKDKGLRATYVGTKN
                     YDIGVNLAKIAMKLKPSGGTICIQSGGAAAANHNERMQGIRDTIAGKKSKEAPGERLT
                     GQNGWKEVDGCPLYTNDDFPLSVTQMGEILAKYPDLTAFVPTGGFPQFVPNAYKQVVD
                     KNMDRIKSKKTILVVADTLPMQMDLMKAGYSHGQVGQRPFDMGYKSMFVLKDLVDKKK
                     VADPIYTGLDVCEPENAATCIAK"
BASE COUNT         1686 a         2363 c         2326 g         1616 t
ORIGIN      
        1 cgcctggcgt gtgcgcttcg cgctgcagaa taccggttgg ctaccgagct atgtgacgaa
       61 aatggccaag aacaggaagc tcgcgcgggg ggttgtatgt gaaatcgaac tgccgaaaga
      121 ggcgcgactc gagaccggca agcagcgaga ggaactgggc cagcttgagg gccgtgcgta
      181 caaatcggct gctgccaata cctgggccgg atccagcgcg gatgaaacgg acgagcgcgt
      241 gagcgtcgaa tgggtcgtgt gcgcgcccat cgggaccagc atcaaactgc ttgcgcgtca
      301 tgagcgggct ggcgtcgcaa ccactcgcgt aacgctcgaa tgaataacct gtcatgacaa
      361 cttcccgggc aaccatcaat accatcggcg cagctttctt cgccacggcg cttccccctc
      421 ttgccattgc cctcggctgg ctcattgtgc cgtcggaaag ccttcctgtt ccgctctgga
      481 ccatgggttg tggcggcgcg gcgctcgctt tcgccggatt agcgctgctc atgccggaat
      541 atttgcccgg gccgcgcggc atacttgcgg cgttgtcgat taccgctctt gccggaattt
      601 tcaattggat tgccttcgga catttccagg gcggatggcg ctttatgctc gagtccttga
      661 tgaattcgct ggccttgcga cacaatgcag cccacaacgt cgtggtgctg ttcgcggtct
      721 tgttgaactt gctggcgttg ctgggctgga cacagtggtt ccgggagatg acgcgcaggg
      781 atggaacgcc cctcaagagc ctcggacgaa aagcccgttc caaacgaaaa ccggcagaca
      841 ccggcgcgtc agcacaggat taaacggacg ggacaccaaa ggaaacggct tatgcggcga
      901 attgctttga tcgtgatgtt catggttgtg gcggcgtaca gcacgacacc aagtcatgcc
      961 gaagatggaa ataagcaaca ttccacagac cgattcaaat cggatgtaaa ggcagtcggc
     1021 aaaggcgcca aagatgcggc cgtggacgtg ggccaccaga tcgccaccgg cacaaagaaa
     1081 gcttataaga gtgcgaaaga caaaatcaag aaagacgcca aagacggcaa gcccggtgat
     1141 ggcagcctcg ccagcaaaaa cgatgcgctg ccagctgtga catcaggcag aaagccgaac
     1201 tagcgcctca tacgaaacga ttctgtgtgg ccggtcacgg cacatcggcc ggcacggcgg
     1261 gacgcgtgta gtgcaaggcg gcggccatca gggcaatgat cagcacattc accacaatga
     1321 ccacgacaat gacccagttc gcttcattga acaactcgaa aatttccaga ggcaggtaaa
     1381 tcgccgcgct gcatgcgacc agccactccc cccaacgcct ttcgaaccat aagccgtacg
     1441 ccatcagaaa gcgaagccct gcataaacgg ctgcaaatcc agcgaaagcc cacagctgcc
     1501 cataggtgac gtgtgaggtg acggacagaa gcatgcgcgc gaagcggctg gcaggattga
     1561 tatgcaatac cgcaatgatt tgcccgccaa catactcaat atagcgatga agtccaagca
     1621 ccaagccgat gccgatgagc aagaccagga ctcccttgaa cgcctcgtaa atcgcgacgg
     1681 cgcgcaacgt tcgagctaac cgcactggca cgctcctcgc acagatttgt cagaacgcag
     1741 cgactggttt gcaaagccgc gatcccggtc cgcgcggact ccattgcaac agttgcgcat
     1801 cgcaaataac ggccgggggt ggaaacgtat agatacctac ggtagccccg agttccgact
     1861 caagtgtgac gcgggaatgc gcttcatccg gagcgcgcca ctggattttg cgctattcga
     1921 catcgacggc ccggccgctg cctagtgact gggtaagtgc aatgccgatg cgagtcgctt
     1981 cagtcgcatc gcgcaaggtc accggcactg gacgatcatc gagcacggcg tccacaaatg
     2041 cttgcgcctc aagctggaag gcttccgcaa aacgctcata aaaagttggc gtgcactcat
     2101 tgcgcactcc ctgggcatcg gatatctcca cccggtcgat gcggggattc tggccgacca
     2161 tcaatcgacc ccgcgtaccc actatttcgg acaacgtttc atgcccatgc gccatggtcc
     2221 gcgatgcata aaagcaggca atttgtccat cgcggaactc gcagatggcc acgccgttat
     2281 cgacgtctcc gcattgagcc agcccctggt ggatcgcgat ggtgccggcg gcatagaccc
     2341 gtacagcggc accagcgcct agaaaccagc gcgcaacatc gatatcgtgg acgcacatgt
     2401 cgagcatggc gccgccgctg gttggagcaa atttcacgaa aaatccgctt ggatcgttct
     2461 ggtcggcggt ttgtgaacgc accaggaaag gccggccaat caaacctgcc ttgactttcg
     2521 cataggcatc gcaatagctg ggatcgaaac ggcggacaaa accaatcatt actttctgct
     2581 ccgagtacct ggcggcttct tcctcgacct tcaggcaatc cggaatattc aacgcgagcg
     2641 gtttctcgga aaaaacgtgc ttgcccgcgt gcaagccctg gacgatctgg tcggcatgca
     2701 acgtggttgg cgtcaccagg aagaccgcat ccaattccgg atgtgcgagc aagtccttgt
     2761 aatcgccgta aagatgtttt acgccaagct tatcgcgcgc ccattccagt tcgcccgcta
     2821 tcgggctgca ggctgcaacc aactcggcac gcagcgtccg gtgcgcaagg ttgaaagcgt
     2881 ggcgttgccc catgcggccg ataccggcaa tgccaacccg aagcggagca ttcatcatgg
     2941 caatctccct gggatatttg ctgcagaaca aacgcggacc atagcgccgg ctccgcggga
     3001 aaaacttagg tagaacgcct gcatttcctg ttgcccagtc cgattgtctt cgatgatacc
     3061 aacggtaaaa agcagtgacg gcaagtttgt ttcgctttac acagggcgct acaacgtgac
     3121 aacgcgcccc tcttgccagg aacgcgttgc cgcatcggca agttcgagcg ccctcacacc
     3181 atcctgcaaa gttgtcttga ccggcgtgcc ttgggccaat gcatcgaaga aatgcgcgat
     3241 ttcgagtgcg tacgcagtgc gataacgctc gaggaagaaa tgctcaggct tgtccgcgct
     3301 gacgctcgac gccgtgtgcg cgaaaacttg cgtcggccgg tggttgccag cctgcaactg
     3361 tcccttcgga ccgagtactt cgaagcgctg gtcgtaacca tacgcggcgc ggcggctggt
     3421 attgatctgg cacaagcgcc cggctctcgt gcgaatcgtg acagcggtgg aatcaacgtc
     3481 ccccgcttcg ccgatttcct tttggacaag gcagctggcg ctggcatgca cactttgcgc
     3541 ttcatccccc agtatccatc ggaacagatc gaagtcatgg atcagcatgt ccctgaaaat
     3601 gccacccgag accttgatat agctgatcgg cggtgggccg ggatcgcggc tggtgatgat
     3661 cagcatttcc ggattgccaa tttcacctgc gtcgagacgc tgcttgaggg cagcgaaagt
     3721 cggatcgaag cgtctctgga atccgatcat gcacagcaca ccggctttgg aaacagatgc
     3781 ggcgcaagcg agcgcgcgct ccagcgccag gtcgaccggc ttttcgcaga agatcgcttt
     3841 gcccgccgct gcggcacgat caataagtgc ggcatgggta tcggtactcg atgcaataac
     3901 gacggccttg atcgccggat ccttgaacgc aacgtcgctg tccgccacac gcgctgcatg
     3961 gcgttgcgcc agttcccccg ccgcggcgcc gttgatatcg attacgtatt tgagccgaac
     4021 cccggattgt gccactagat tggcgccgtg gatcttgccg atgcgcccag cgccgaacag
     4081 cgccacttcg atcatgtttc ctccgggtca attatttccc tgcggcgcga attgctgcat
     4141 cgcggtgcaa tgacgttgca ccaaggggca aggaagattg tacgaagttt tagccagggt
     4201 ggaaaatatc gacttctacc ggggaatgaa tcaacgacgc gcccgtgcat cgttcatggg
     4261 gcttacggag gcaggcatgg gaggaaagat ccgcttgacc atggcgcagg cactcgtgcg
     4321 ctatctcgct cgcttgcgca ccgatctgcc gggcgatgtg ccgctgtttg gtggcgtgtt
     4381 cgcaatcttt ggtcatggca atgtcgccgg catcggcgaa gcgctgtatc aggtgcgcga
     4441 ccagttgccg acataccgct cgcacaatga acaggcgatg acactgagcg cagtcgcttt
     4501 cgccaaatca catatgcgcc gtcgcatgat ggcttgcacc acatcgatcg ggccgggggc
     4561 aacaaacatg gtgaccgcgg cagcggttgc ccacgtcaac cgattgcctg tgctgttgct
     4621 tcccggtgac atcttcgtct cgcgcgcacc ggatccggta ttgcagcaag tggaggacct
     4681 gcacgacggt ggcatatcgg cgaacgattg cctgaagcct gtttcgcgtt atttcgaccg
     4741 catcgtgcat ccgcaacaat tgctgactgc gctaccgcgc gccattcacg tgctgaccga
     4801 tccggcgttg tgcggtccag tcacgctggc cctgccgcaa gacgttcaaa cgatggcgta
     4861 cgactggccg gaagaattct tttctccgca caggatcacg ctgcgcatgc tgtctcctgc
     4921 gccttcagaa ttgcgcgaag cggccgctac actgaaacag gcaaagcggc ccctattggt
     4981 tgcgggcggc ggcatccttt acggcgacgc tactggcgta ttgcgcgact tcgccgaggc
     5041 gcatggtgta ccggtagcgg aaacgcaagc cggaaaaggc gctctgccat gggatcacgc
     5101 attgcaactt ggctcgttgg gcgtcaccgg atcgaccgcc gccaatgcaa ttgcgcgcga
     5161 tgccgatctg gtgttcgcgg tcggcacgcg gctgcaggat ttcacaactg gatcacattc
     5221 gctattcact caggcaaaac tgctgaacct caacgtcaac ggcttcgatg cgaccaagct
     5281 gggcgggttg ccactgcagg ctgatgcacg ttccggtttg caggcactcg acatggcgct
     5341 gcccggctgg aaagccgacg cccgctggac ggcacaagcg gcgcagttgg caacgcagtg
     5401 gcggacaacg gtagattcaa tcaccggcaa ccgggacgtc aagctgcctt acgagggaga
     5461 agtcatcggc gcggtacagc gttcctcacc gaactcggcg gaacgagaca tcgtggtgtg
     5521 tgccgctggg ggtctgcccg gcgaattgca taagttgtgg cgcacatcgc tacccggcgg
     5581 ctatcacctc gaatacggtt actcgtgcat gggttacgag atcgccggag ggcttggcgt
     5641 gaaaatggcc cggccggatc gcgaagtcat cgtcatcgtc ggtgacggca gttatctgat
     5701 gatgaattcc gaaatcgcca cgtcgatcat gctcggcctc aaactcgtga tcgtgttgct
     5761 cgataaccgt ggatacggct gcatccaccg cctgcaggaa gcctgtgggg gtgccccctt
     5821 caacaacctg ttcgacgatg gagttgcaac agcaaacggc gcaccgcgca tcgatttcgt
     5881 cgcgcacgcg cgcgcaatgg gcgctaatgc ggaaatggcc gacgacatcg gcgcgctgga
     5941 ggcagcgctc aggcgcgcac gcgctgccaa ccgaacttcg ctggtgtgca ttgccaccga
     6001 cccgaagcgt acgacgctgg aaggcggctg gtggtgggaa gtcgcggtac cggaagtgtc
     6061 gtcacgcagt gaggtcacag tcgcacgcaa gtcttacgaa caaggcaagc agcagcaaaa
     6121 accatgagcc gtaattcatt cgacgtaaag atcgggatca atcccatatc gtgggcgaac
     6181 gatgatctac ccgaactcgg cggcgacacg ccactggagc gcatcctgtc agaaggcagc
     6241 gccatcggct accggggctt tgaacttggc aataaattcc cccgcgagcc ggagatgctg
     6301 cgcaaagtgc tcgcacaata tggggtggaa ctggtgtccg ggtggtattc cggccaactt
     6361 gcacggcgtt ctgtagacga cgaaatcgct gccgccagtt cgcatgtgca attgctcgcg
     6421 aagaatggcg ccaaggtgat ggtctacggc gaagtcgccg attccatcca gggcaagcag
     6481 gacatgccac tatcgcggcg cccccagttc actggcgata cgcaatggcg cgattatgca
     6541 ggcaggcttg acgccttcgc tcactacacg tcatcacgag ggatcaagct ggcctaccat
     6601 catcatatgg gcgcttacgt gcagtccggc gcggacattg accgcctgat gtcgctcacc
     6661 ggctcgcagg tcggcctgtt gctcgacagc ggccacctaa gctttgccgg ctctgatcca
     6721 ctggctgtgc ttaacaagca catcgcccgc gtttgtcacg tgcattgcaa ggacgtgcgc
     6781 cccagcgtac tggggcaagc gtacaaagag gactggagct ttctgcaggc ggtattgaaa
     6841 ggagtcttca ccgttcccgg cgatggcgcg atcgatttcc gatcgcttct ggtccggctg
     6901 caacagcacg gctatcgcgg ctggctggtg gtcgaagccg aacaggatcc tgcgctggca
     6961 cccagctacc aatatgcgca gatgggattc cgccatctgt ccgcgctggt caattcgtac
     7021 gttgccgggt aaaaatgcgc tgcataaaaa ggccaccgca cgagcggtgg ccttttttgg
     7081 accctgcttt tacttcgcga tgcaggttgc cgcgttttcc ggctcacaaa catcgaggcc
     7141 agtatagatc ggatctgcaa ctttcttttt gtccaccaag tccttgagca cgaacatcga
     7201 cttgtagccc atatcgaagg gacgctgccc gacctggccg tggctgtatc cggccttcat
     7261 cagatccatt tgcatcggta gcgtgtccgc aacgaccagt atggttttct tgctcttgat
     7321 gcggtccatg ttcttgtcta ccacctgctt gtaggcattc ggcacgaact gcgggaagcc
     7381 acctgtgggc acgaaggccg tgaggtcggg atactttgca agaatttctc ccatctgggt
     7441 taccgacaac ggaaaatcat cgttggtgta cagcgggcag ccatcgactt ccttccagcc
     7501 gttttgaccg gtcagtcgct cgccgggcgc ttccttggat ttcttgcccg cgatggtgtc
     7561 gcgaatgccc tgcatgcgtt cattgtggtt agccgctgcg gcgccgccgg actggatgca
     7621 aatagttccg ccgctgggct tcaacttcat tgcgatcttc gccagattca cgccgatatc
     7681 atagtttttg gtaccgacat aggtcgcgcg caatcccttg tccttcggca acagatcgga
     7741 atcccatgta gtcaccggaa tcccggccgc tttggcgcgt tcaagcacct ttgccatggc
     7801 aggggcgttg gacggcgaga cggcaatgcc gttgactttg gcggtgatca gatcctgcac
     7861 gactttaact tgctcgtcgc cgccaccgtg ctctcccggt ccgatatacc ggcactcgat
     7921 tttgcctgca ctttccttct cggctttttt gcaaccgtcc cgtgcctggt cgaaaaacgg
     7981 attgttcatg t
//
LOCUS       DATMZQ010000025        13801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3815482, whole genome shotgun sequence.
ACCESSION   DATMZQ010000025 DATMZQ010000000
VERSION     DATMZQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3815482"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VN496_00835"
     CDS             <1..652
                     /locus_tag="VN496_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF15514.1"
                     /translation="YAAGEESLASFSEGFAAAGGAVVKELFVPFPTVEFQALLTEIAS
                     VKPDAVACFFAGGGAVKFVKDYAAAGLKEKIPLYGSGFLTEGVLPAQGDAAEGLLTTL
                     HYADSLENPRNKAFRSAYAKAYKTEPDVYAVQGFDTGLLLAQALDKTKGDIGNQKAVI
                     SAMEGAVIDSPRGQWKMSRAHNPIQDIYLRRVEQGDNKVIAVAWKALEDPARGCRL"
     gene            758..1654
                     /locus_tag="VN496_00840"
                     /pseudo
     CDS             758..1654
                     /locus_tag="VN496_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            1651..2622
                     /locus_tag="VN496_00845"
     CDS             1651..2622
                     /locus_tag="VN496_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF15515.1"
                     /translation="MITRPRTVTLVALLLLIALVLLPLLAEKFTVQFATRILIMALFA
                     MSLNLLVGSGGLVSLGHAAFFGIAGYCLALLSPPDHGANFWTSLPLSMMAAGALALVI
                     GALVLRTSGIYFIMATLAFAQMLYFLLHDTGIAGGSDGIYIYVRPDANLFGWHPFDLE
                     KFRDFYFVVLSVFVLVLIFLGALRASLFGRVIAGIRVNERRMRSLGFATLRYKLVCFV
                     LAGILAGLAGYFSAAQFGVVNPDMLGWHLSGAALMMVILGGMGTLAGPALGAAVMLLL
                     ELGLQSLPAAGGIDLGKHWQLPMGIVIVLVALYLPAGIARRFGEADE"
     gene            2615..3367
                     /locus_tag="VN496_00850"
     CDS             2615..3367
                     /locus_tag="VN496_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15516.1"
                     /translation="MSEIILRTHALSRHFGGLAALADVSLACETRQVHAVIGPNGAGK
                     STLINVLSGDLPPNAGEVQFHGRDITGFSADQISQLGIARSYQKTNLFPSFTVFENCR
                     LAAQSRLPSSMHFFRPAAAQADVNARARDALSQAGLAGRSDIVASELSHGEQRQVEIA
                     MALATRPCLLLLDEPLAGMGTEESMRIVSLLRELARDHAILLVEHDMDAVFAIAHTLT
                     VMVNGRVLASGTPEQIRSSRAVQEAYLGVANA"
     gene            3360..4073
                     /locus_tag="VN496_00855"
     CDS             3360..4073
                     /locus_tag="VN496_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15517.1"
                     /translation="MPEALLEATGITAGYGASRVLHGVDLALKRGEAVGLMGRNGMGK
                     TTLLRALLGLLPLQAGRVKVKGTDISGWAPHRIARSGVAYVPEGRGIFADLSVEENLV
                     MAARPGIDNQRDWTLERVLGIFPRLAQRLRHGGQQLSGGEQQMLTIGRALMTNPDVLI
                     LDEATEGLAPLVAQDIWAILKQLREAGLSTIIVDKNFAAVSALTDRNIVLVKGRIALQ
                     GSAVDLQRDPATLQRHLGI"
     gene            4074..4853
                     /locus_tag="VN496_00860"
     CDS             4074..4853
                     /locus_tag="VN496_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF15518.1"
                     /translation="MLAAGGHRLEYALLEPQDAQAVDRPMLVFLHEGLGSVALWKDFP
                     AEVVAATGCPALVYSRYGYGKSETLTAPRSVDYMHREALDVLPEVLDHFGVRSPILIG
                     HSDGASIALVHAGSGRRLVKGVVAMAPHVFVEDITIKSIAEAKTAFQHTDLPQKLSRY
                     HDNVVSAFGGWNDIWLHPEFRRWNIEDYLPGIVVPVLLMQGAEDQYGTVAQIAAIARQ
                     VSGPIETIMLPGCGHSPHQSPQKTAVIVAIARFVAKLRESA"
     gene            4899..5681
                     /locus_tag="VN496_00865"
     CDS             4899..5681
                     /locus_tag="VN496_00865"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HXF15519.1"
                     /translation="MLKGKSAIVTGSTSGIGLGIARALAAQGCNVMLNGFGDSTQIEK
                     LRAGLAAEHKVVALYSSADMSKPLEVAAMVDQAARRFGAVDVLVNNAGIQHVAPVDEF
                     PIEKWDQILAINLSSAFHAIRSALPGMRRRKWGRIINIASAHGLVASPFKSAYVAAKH
                     GLLGLTKTVALEAAGSGITCNAICPGYVLTPLVEKQIEDHVKARGIAREEAISNVILE
                     RQPSKEFVKVDEIAALTVFLAADQAASITGAAYSIDGGWTAQ"
     gene            5815..6213
                     /locus_tag="VN496_00870"
     CDS             5815..6213
                     /locus_tag="VN496_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXF15520.1"
                     /translation="MDIVRRFGPLVGRILLALIFIISGFGKIMGFDATLGYIRSAGLP
                     FAQLSAMAAIVVELGGGILLVLGWKARWAAAALFVFVLVAALYFHAFWASPPDQAMMQ
                     QIQFMKNLAIMGGMLYIVAYGSGPYSVDKT"
     gene            complement(6251..7426)
                     /locus_tag="VN496_00875"
     CDS             complement(6251..7426)
                     /locus_tag="VN496_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF15521.1"
                     /translation="MTDRRMIAMLLALCSATFFVTSAGASMAPFLNLIAGSLGTTLPD
                     IAHLFSFQALMWGIASLGAGMVSERIGRRVMLVGGVLIMGVMRLGFSASHSYPGAVMW
                     QVLSGAGGGVFMGVVYAAVAEHAPAGMRGRAMSWVITGQSLSLVLGVPLVTLLGAFGG
                     WRGAIAAHGTFVLLCAIAVWLATPVDPPRHPHAAQRKTPFAALLKPQLVALLAAGTTE
                     RMCFAVVAIFLPAFLQDAYGTSLGALALVLALVAAGNLVGNILGGRIADRTRSRARMF
                     ALSSALTATLALPLLSWHPGLFASVTLGFVFSFVNAAGRPSLMATLGEVPSELRSALF
                     GLNITMASLGWLLAGSVGAWLIAVGGFAGLGAFCAFVAALGCGFALFSSARSRAPSA"
     gene            complement(7610..9127)
                     /locus_tag="VN496_00880"
     CDS             complement(7610..9127)
                     /locus_tag="VN496_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF15522.1"
                     /translation="MAVQYPTPDQIWDAAEEIGLDLTEADVKSYLGLIKPNIDAYNMV
                     DAMPDNLPAVKYPRTPGYFPDASQNSRNAWYVRTSIKGASSGVLKGKKVAVKDNVMVA
                     GVPMMNGNSILEGYVPDIDATVVVRLLDAGAEIIGKTHCESYCISGGSHTGAKGAVHN
                     PHKMGYSAGGSSSGSGVAVALGEADIALGGDQGGSIRMPSSWCGIYGMKPTHGLVPYT
                     GIMPIEIYVDHTGPMSATVSDNALMLEVIAGPDGYDPRQYGTPKKVKYSKMLDGGVGG
                     MKIGVVREGFGRPESEAASDAKVKKAAELYKKLGAKVEEISIPAHLIGPVLWTPIGVE
                     GITQTMMLGDGYGLSRPDLYVTSLIDKLHGWELRANELSETTKVLTTLGVYIKKYHGT
                     HYYGKAMNITRRLIEAYDAELAKYDLLLMPTMPMKAQPLPAPNCPREEYFQRALEMIG
                     NTCPFDITHHPAMTVPCGFVDGLPIGMMLIGKHYDEATIYRAAYAFEQTGDWKQM"
     gene            complement(9189..9908)
                     /locus_tag="VN496_00885"
     CDS             complement(9189..9908)
                     /locus_tag="VN496_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15523.1"
                     /translation="MPEVPHLNAPLLDANGVCTFYGTSQVLFDVSLKAPARGVVAILG
                     RNGAGKTTLLKTLAGDLLPARGVVRFNGSDVARLPTERRVRLGIGYVPQEQAVFGRLS
                     VRENLLIGAMGAADSKDRIGDMLTLFPLLAKRLSQQAGTLSGGERKMLGISRALLGRP
                     SLLMLDEPTEGVWHGVVGEIAARLEELAKTIAVVIVEQHVKMALQVSQYCYVMDRGQI
                     ALEGPSAKIHDNPELIRLLSP"
     gene            complement(9901..10635)
                     /locus_tag="VN496_00890"
     CDS             complement(9901..10635)
                     /locus_tag="VN496_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15524.1"
                     /translation="MNLLEARNVTKIYGALTALNGVDLTVRDKEFHGLIGPNGSGKST
                     LLKCIAGAEMPDAGTIGFLGDDIGFAAPSERARAGLSIKFQIASVLPQLSVYDNILLA
                     VQAHDSLARLLFSHSRRALDKQVIRTLEQFRLSARKDDLAGVLSHGEQQWLEIAMALA
                     GKPKLLLLDEPTAGLSSQERRATGELLAPIKQICSLLIVEHDLDFIKDLCDSLTVLDQ
                     GQVVASGPTTQVQNSDKVKEAYLSHA"
     gene            complement(10640..11686)
                     /locus_tag="VN496_00895"
     CDS             complement(10640..11686)
                     /locus_tag="VN496_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF15525.1"
                     /translation="MSHPTKPSIAPRPLRKIIPWLELILLAASLIAPVFADDFWTLFC
                     TRVFILGLLALSFDLVWGYAGILSFGQALFFGMAGYCTALLATEGGITSVLVLLPVAG
                     LVGLVVSLILAFIVILGRRVPSTVFVALGTLTGSYVVERLARGWSYVGGQNGIPSLPR
                     LTLAGHEIAEGVSFYYMALGVLLISYVALKFLTRSQLGLVLAGIRQHETRITFFGYRV
                     QHVKGIIFALGGFIAGLAGGMYAFHEGYVGPGQLSPVLSTQVVLYVLFGGVGTLIGAV
                     SGAGMIEYLSFMLSQQYQRLWPIILGLLLLIIVMFRPTGLISFFASDRERIGSFGRPD
                     EAALPQNDADQKPA"
     gene            complement(11779..13011)
                     /locus_tag="VN496_00900"
     CDS             complement(11779..13011)
                     /locus_tag="VN496_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HXF15526.1"
                     /translation="MKNDQCRNRPLSASRRLFLQRTSLLTGMVALGGGSYLYNPKGVW
                     AADPIKVGIATDLTGAISWGGIPNSQIAKMVIDDINQGGGLLGRPLQMILEDTATNEQ
                     LAVTKVRKLVTQDKVDVVFGGITSSMRNAIKDTIVNRGKTLYIYPQLYEGKECTPFLF
                     CTGPTPAQQCDTFIPWLIKNGGKKFYLPSADYVWPHTLNAYARTVITKNGGEVVGEEY
                     FPVDHNEYSATVNKIMSSGVNVVFNTTIPPGVAAFFKQLNEAGFQKKGGRLSCVYYDE
                     NALGITPAHEIEGLASCLDYFKAVNDPFGNKLQAEYGKQFGDKNPFTAGSGATGMYRG
                     IRLWEAAVKEAKSVKREPVAKALDHAKISDGPGGGCEMVPGTRHVKMNMYTAVSKGGK
                     YEIVEKSKGPVMPGECGA"
     gene            complement(13015..>13801)
                     /locus_tag="VN496_00905"
     CDS             complement(13015..>13801)
                     /locus_tag="VN496_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF15527.1"
                     /translation="LGVIASMMGIFNFAHGEFVLLGAYTVYLFQSRGLPVWAGMAVAP
                     IAVGIAGLVLERTVIRRFYATPIVAMLGTYAIGLALRETIRSLLNGQFYSVTAPLVGS
                     FSFGEISISQWRTAVIVITAAVMVACYLLLTRTRFGLQVRASLENPALARASGISTRR
                     VYAATFAFGAALAGLAGGLMVPIYAISADMGVPFLIKSFLAVMLGGMGSFEGAIAGAA
                     LVGATSAALPWAIKPVVADVVVFIIAVAIVKVRPEGLLSKWRK"
BASE COUNT         2800 a         4032 c         4053 g         2916 t
ORIGIN      
        1 gtacgcggct ggcgaagaat cgctggcttc cttcagcgag ggttttgccg ccgctggtgg
       61 agcggttgtg aaggaactgt tcgtgccgtt tccgaccgtc gagttccagg cgctgttgac
      121 cgagatcgca tccgtcaagc cggatgcggt cgcttgcttt tttgccggtg gcggcgcggt
      181 gaagttcgtc aaggactacg cggccgcagg actgaaggaa aaaattccgc tgtacggctc
      241 cggcttcctc accgagggtg tactgccggc gcagggagat gcggccgaag gtttgctcac
      301 caccttgcac tacgccgact cgctggaaaa cccgcgcaac aaggcgtttc gctccgccta
      361 tgccaaagcg tacaagactg agcccgacgt ctacgctgtt cagggctttg acaccggctt
      421 gctgctggcg caagcgttgg ataaaaccaa aggcgatatc ggcaaccaga aagccgttat
      481 ttcagccatg gaaggagctg tcattgatag cccgcgcggc caatggaaaa tgtccagggc
      541 gcacaatccg attcaggata tctatctgcg acgcgttgag cagggcgaca acaaagtcat
      601 cgcggtggcg tggaaagcgc tggaggatcc cgcccgaggg tgtcgcttgt agggaagagg
      661 tgaagtacca gaggcgctaa gcccggggtg ggtggaccat ttcacacctt catctttcac
      721 tggttttgca tgaacgcctt tgctaatgct ttagacgatg gacctctcga cgttcctgat
      781 ccagttgctc aacgccatgc agtatggact gctgctgttc ctgctcgcgg caggactgac
      841 tctgattttc ggcatcatgg gtatcatcaa tctggcccat ggcagctttt acatggttgg
      901 ggcgtacctg gcgtggtcgc tatcaatgca actgggcagt ctgtggcttg caattccggc
      961 aggcatcgta ttgtcggtgc tgctgggcat tgcgctggaa tggctgttga tccggcggct
     1021 gtatcgtctc ggtcatctgc aacaagtctt gctgacgtac ggattgattc tgatcgtcga
     1081 ggaattgcgc agcattatct agggcgatga ggtacacggt gtcccagtac cgcagctgct
     1141 gtcagcatcg attccgctga ccgatacact ttcttatccg gtctaccgag tatggatctc
     1201 ggcggtatgt ctgctgcttg ccttgttgct ttatgtcctg atccagcgca cgcgcctcgg
     1261 catgacaatc cgggcgggtg cgagcaatcg cgagatggtg caatcgctgg gtatcgacat
     1321 cgatctgctg taccgcttcg tgttcgcgct cggcgtggcg ctggctgcat tcgccggcat
     1381 gattgcagcc cccgtgagtt ccgtgtttcc cggcatgggc aaccatgtcc tcatcctttg
     1441 tttcgtggtg gtcgtaatcg gcggcatcgg ttcggtcaag ggtgcgctaa tcggtgcact
     1501 actgattggt ctggtcgata catttggcaa agtcatcgag ttcaaagttg gaaattttca
     1561 gctgttgccg cagttgtccg gcatgagcgt ctatctgctg atggccgctg tgttgctgtg
     1621 gcggcctgcc ggactgttcg gtagaaatgc atgatcacgc gcccgcgtac tgtcacactt
     1681 gttgcgctgc tgcttctcat tgctctggtc ttgctgccat tgctggccga gaaattcaca
     1741 gtccagtttg ccactcgcat cctgatcatg gcgctgttcg cgatgagcct gaaccttctg
     1801 gtaggaagcg gtggactggt cagcctcggc catgcggcgt tctttggcat agcgggctac
     1861 tgcctggcgt tgttgtcacc gccggatcat ggagcgaatt tctggaccag cctgccactc
     1921 tcaatgatgg cggccggcgc acttgcgctg gtgatcggcg cgctggtgct tcgcaccagc
     1981 ggcatctact tcatcatggc gacgctggcg tttgcgcaga tgctctattt cctgttgcac
     2041 gataccggga tcgcgggggg ttccgatggc atctacattt acgtccgtcc cgatgcgaac
     2101 ctgttcggct ggcacccgtt cgatctggaa aaattccgcg atttctattt cgtggtcctg
     2161 agcgtgtttg tgcttgtgtt gatattcctg ggcgcattac gtgcttcgct gttcgggcgc
     2221 gtcattgccg gcatccgcgt caatgaacga cgcatgcgct cgctcgggtt tgcaacgttg
     2281 cgctacaaac tcgtatgttt tgttctcgcc ggtatcctgg cgggcctcgc gggctatttc
     2341 tcagcggcgc aattcggcgt agtcaatccc gacatgctgg gctggcatct gtccggcgct
     2401 gcactgatga tggttatcct cggcggcatg ggcacgctgg cgggtcctgc actgggtgca
     2461 gccgtgatgc tgctgctgga actcggtttg caatcacttc cagcagcggg tgggatcgat
     2521 ctcggcaaac actggcagtt gccgatgggc atcgtcatcg tactggtcgc cctgtatctg
     2581 cctgccggca ttgcgcgtcg gtttggcgag gcggatgagt gaaataatcc tgcgaacgca
     2641 cgccttgtca cgccattttg gtgggctggc cgcgctcgcc gatgtgtcgc tcgcctgcga
     2701 gacgcggcag gtacatgcgg taattgggcc gaatggtgca ggcaaaagta cgctcatcaa
     2761 tgtgctgtcc ggcgatctgc cgccaaatgc cggcgaagtt cagttccacg gccgcgacat
     2821 caccggcttt tcagccgacc agatttcgca gctgggcatc gcacgcagct accagaagac
     2881 caacctgttt ccatccttta cggtattcga gaattgcagg ctggcggcgc aatcccgttt
     2941 gccaagctcc atgcatttct tccgcccggc cgctgcgcag gcagacgtca atgcacgcgc
     3001 ccgcgatgcg ctatcgcagg cggggcttgc cggacgcagc gatattgtcg catcggagct
     3061 ctcgcatggt gaacaacgcc aggttgagat tgccatggca ctggccaccc ggccttgctt
     3121 gttgctgctg gacgagccgc tggcgggaat gggcacggag gaatccatgc gcatcgtgag
     3181 cttgctgcgc gaactggcca gggatcacgc catccttctg gtcgagcatg acatggacgc
     3241 cgtattcgcc attgcccata cgttgacggt catggtgaac gggcgcgtgc ttgcctcggg
     3301 tacgccagaa caaatccgct ccagtcgggc ggtacaagag gcttatctgg gcgtcgctaa
     3361 tgcctgaggc attgctcgag gcgacaggca tcactgccgg ctacggcgca agccgcgtac
     3421 tgcatggtgt ggatctggcg ttgaagcgcg gtgaggctgt tggtctcatg gggcgcaacg
     3481 gcatgggaaa gaccacgctg ctgagagcat tgctgggatt gctgccgttg caggccggtc
     3541 gcgtgaaagt aaagggcacc gatatatcgg gttgggcgcc acaccgcata gctcgctcag
     3601 gtgtcgccta cgtgcccgaa ggacgtggca tctttgcgga cttgagcgtc gaagagaatc
     3661 tcgtcatggc ggcgcggccc ggtatcgata atcagcgcga ctggacgctg gaacgcgtat
     3721 tgggaatctt tccgcggctc gcgcaacgct tgcgtcacgg cgggcagcaa ctttcgggcg
     3781 gtgaacagca aatgctgacc attggccgcg cgctgatgac caatcccgat gtgctgattt
     3841 tggacgaggc aacggaaggc ctggcgccac tcgtcgcgca agatatttgg gctatcctga
     3901 agcaattgcg tgaagctggc ctgtcgacga ttattgtcga caagaatttt gcggctgtgt
     3961 ccgcgctcac ggaccgcaac atcgtgctgg tcaagggacg catcgcattg caaggcagcg
     4021 ccgtggacct gcagcgcgac ccggcgactt tgcagcgcca tctgggaatc tgaatgctcg
     4081 ccgccggtgg ccatcgtctc gaatatgcct tgctcgaacc gcaagatgca caagctgtcg
     4141 accgcccgat gctggtattc ctgcacgaag gcctgggctc ggttgcgctg tggaaagatt
     4201 ttccggccga agtggttgcc gcaacgggtt gtcccgcgct ggtgtattca cgttacggtt
     4261 atggcaagtc ggagacactg actgcaccac gcagcgtgga ttacatgcat cgggaggcgc
     4321 tcgacgtgct gccggaagtg ctggaccact tcggcgtcag atctccgata ttgatcggcc
     4381 atagcgatgg tgcatcaatt gctctggttc atgccggttc cggccggcgg ctggtgaaag
     4441 gcgtagtcgc catggcaccg cacgtgttcg tcgaggacat taccatcaaa agtatcgccg
     4501 aagccaagac tgcttttcag cacacggacc tgccgcaaaa actcagtcgc tatcatgaca
     4561 atgtcgtttc cgcttttggt ggctggaatg acatctggct gcatccggaa ttccgccgtt
     4621 ggaacataga agattacctg ccgggtatcg tcgttccagt gttgttgatg cagggagcag
     4681 aggaccaata cggcacagtg gcccagatcg ccgcgatcgc gcgccaggtc tcgggaccga
     4741 tagagacgat catgctgccg ggttgcggtc attcgccgca ccagagtccg caaaagaccg
     4801 cggtcattgt ggcaatcgca aggttcgtgg caaagctgcg cgaaagcgcc tgaggcgtct
     4861 gttaaactgg attcaaaccc agtggaatat gtttcatcat gttgaaaggg aaatccgcca
     4921 tcgttaccgg gtccaccagc ggcatcgggc tcggcattgc gcgcgcgctc gcggcgcagg
     4981 gctgcaacgt catgctcaac ggtttcgggg attcgaccca gattgagaaa ttgcgtgccg
     5041 ggcttgccgc cgaacacaaa gtcgtcgcac tctattcttc cgctgatatg agcaagccac
     5101 ttgaagtggc ggcgatggtg gaccaggcgg caaggcgatt cggtgccgtg gatgtgctgg
     5161 tgaacaacgc cggcattcag catgtggcgc cggtcgacga atttccgatc gagaaatggg
     5221 atcagatcct tgcgatcaat ctgtcctcgg cgtttcacgc tatccgtagc gccctgcctg
     5281 gcatgagacg acgcaagtgg ggccgcatca tcaacatcgc atcagcgcat ggcctggttg
     5341 cttccccatt caagtcagcg tacgtggcag ccaagcacgg gctgctcgga ttgaccaaaa
     5401 cggttgcgct agaggcagcc ggcagcggca tcacttgcaa cgcgatctgt cccggctacg
     5461 tgctcacacc gttagtggaa aaacaaatcg aggaccatgt gaaggcgcgt ggcattgcgc
     5521 gcgaagaagc catcagcaac gtcattctcg agcgccagcc gtcgaaggaa ttcgtcaaag
     5581 tggacgaaat cgccgcgctt accgtattcc tggccgccga tcaggctgcg tcgatcacgg
     5641 gcgccgcgta ttcaatcgac ggtgggtgga cggcgcaata acgaatgtag caaacagaag
     5701 aaaacggtcc cccaaagggt ataccggtag cgagcgtgaa gccagctttg gctggtctgg
     5761 aacgtgatca gcaacggcag taaatttata ttctttcagt aaaggggacg tgccatggat
     5821 attgtcagaa ggttcggtcc gctggtgggc aggattctgc tggcgctgat ttttatcatt
     5881 tccggtttcg gcaagataat gggtttcgat gcaacgctcg gctacattcg ctcggcaggt
     5941 ctgccgtttg cacaactcag tgcgatggct gcgattgtgg ttgagttggg gggcggcatc
     6001 ttgctggtgc tgggctggaa ggcacgctgg gccgcagcag cgttgtttgt ttttgtgctg
     6061 gtcgctgcgc tttattttca cgccttctgg gcttcgccgc ctgatcaggc catgatgcag
     6121 cagattcagt tcatgaagaa cctggctatc atgggcggca tgctgtatat cgtggcatat
     6181 ggttcaggcc cttacagtgt ggacaagact tgagcgccac gtcgcgtgtc tcgccgctag
     6241 cgaccagcca ctaggcactc ggagcacgcg accgcgcgct gctgaatagc gcaaatccgc
     6301 agccgagcgc ggcgacgaac gcgcagaatg cacccagtcc ggcaaagccg ccgacggcga
     6361 tcagccaagc accgaccgac cccgccagca accagcccaa actggccatg gtgatattca
     6421 gcccgaacag cgcgctgcgc aactcgctgg gcacctcgcc gagcgtggcc atcagcgacg
     6481 gccggccggc ggcgttgacg aaggaaaata cgaagcccag tgtgacggaa gcaaagaggc
     6541 ccggatgcca gctcagcagt ggcagtgcga gcgtcgcggt cagtgcggag ctcagcgcga
     6601 acattcgcgc gcgtgagcgc gtgcgatcgg cgatacgccc gccgagaatg ttgccgacca
     6661 gattgcctgc cgccaccagc gcgagaacca gcgccagcgc gcccagcgaa gtgccatagg
     6721 catcctgcag aaacgcgggc aggaagatcg cgacgacggc aaagcacatg cgttcggtgg
     6781 tgcctgccgc aagcagcgca actagctgcg gtttcagcag tgcggcaaac ggcgtttttc
     6841 gctgcgcggc atgcggatgg cgcggcggat caacaggcgt cgccagccat accgcaatag
     6901 cgcacagcag gacgaacgtg ccgtgtgccg cgatcgcgcc gcgccagccg ccgaaagcgc
     6961 ccagcaaagt caccagcggt acgcctagta ccagcgacag agattgtcca gtgataaccc
     7021 agctcattgc gcgtccccgc atgccggcgg gcgcgtgttc cgcgacggcc gcataaacga
     7081 cacccatgaa cacgccaccg ccagcgccgc tgagtacctg ccacatgact gcgcccggat
     7141 aactgtgcga agcggaaaat cccagccgca tgacgcccat gatcagcacg ccgccgacaa
     7201 gcatcacgcg gcgtccgatg cgttcggaaa ccatgccggc gccaagcgaa gctattcccc
     7261 acatcagtgc ctggaacgaa aacaagtgcg caatatcagg cagcgtggtc cccaggctgc
     7321 ccgcgatgag attcagaaac ggcgccatgc tggcgccagc agaggtgacg aagaaagtcg
     7381 cgctgcacag tgcaagcaac atcgcgatca tgcgacgatc agtcatgaga tcctgcagac
     7441 agttggaagg taaaaatggg gagccgctgg ctagcggcta gtgtggcttg tgattgttat
     7501 ggaaaaattc cggcacaaaa taacaagagg cacacgaggt gcgcctcttg attctaccca
     7561 ctaaccaccg gtcaccaacc aatccagcca ccagccagcg aatctataac tacatctgtt
     7621 tccagtcacc ggtctgttcg aacgcgtagg ccgcacgata gatcgttgct tcgtcgtagt
     7681 gcttgccaat caacatcatg ccgatcggca gcccgtccac aaagccgcaa ggtactgtca
     7741 tggctggatg atgtgtgatg tcgaatgggc aggtgttgcc aatcatctcc agcgcgcgct
     7801 ggaaatattc ttcgcgagga cagttaggag caggcagcgg ctgcgctttc atcggcatgg
     7861 ttggcatcag caacaggtcg tactttgcga gctcggcatc ataggcttcg ataaggcggc
     7921 gcgtgatgtt catcgccttg ccgtaataat gggtgccgtg atactttttg atataaaccc
     7981 ccagggtggt cagcaccttg gttgtttccg atagttcatt ggcgcgcagt tcccagccat
     8041 gcagcttgtc aatcaggctg gtgacataaa ggtccggccg gctgagtccg tatccatcgc
     8101 cgagcatcat ggtttgcgtt atcccttcca ctccgatagg cgtccacagc accgggccga
     8161 tcaggtgcgc cggaatggaa atctcttcta ctttcgcgcc cagttttttg tacaactcgg
     8221 cggccttttt gactttggcg tccgaagcag cttcggattc gggccggccg aacccttccc
     8281 tgaccacacc gattttcatc ccgcccacgc cgccgtccag cattttggaa tacttgacct
     8341 tcttcggcgt gccgtattga cgtggatcat aaccgtccgg gccggcgatg acttcgagca
     8401 tcagcgcatt atcactgacg gttgcggaca tcggtccggt atggtcgaca taaatctcga
     8461 tgggcatgat gccggtgtag ggcaccagtc cgtgcgtggg cttcatgccg taaatgccgc
     8521 accaggaaga aggcatgcga attgagccac cctggtcgcc acccagcgcg atgtccgctt
     8581 cacccagcgc taccgcaaca ccgctgccgg acgaagagcc gcccgcggag taacccatct
     8641 tgtggggatt gtgaacggct cctttcgcgc cggtatggct ccctcccgaa atgcaatacg
     8701 actcgcaatg cgttttgccg atgatttctg cgccggcatc cagcagccgc acaacgacgg
     8761 ttgcatcgat gtccggcacg taaccctcca gtatcgaatt gccgttcatc atcggcacgc
     8821 ccgccaccat gacattatct ttgacggcga ctttcttgcc tttcagcacg ccggacgagg
     8881 cgcccttgat tgacgtcctg acgtaccagg cgttgcgcga gttttgcgaa gcatcgggaa
     8941 agtaccccgg cgtgcgcgga tacttcaccg cgggcaggtt gtccggcatg gcatccacca
     9001 tgttgtacgc gtcgatattg ggtttgatca atcccaggta ggatttcacg tcggcctccg
     9061 tgagatccag tccgatttct tcggcggcgt cccagatttg gtcaggtgtc gggtattgca
     9121 ctgccatgat ggtctcctct ttataaggtt gaacgagatg tcttgcggcg cgttctttcg
     9181 cgcattcttc agggagaaag cagccggatc agttccgggt tgtcgtgaat ttttgccgaa
     9241 ggtccttcca gcgcgatttg accgcgatcc atgacgtagc agtactgaga gacttgcagt
     9301 gccattttca catgctgttc gacaatcacc actgcgatcg tctttgcaag ttcttccagg
     9361 cgcgctgcga tttcgcctac gacgccgtgc catacgccct cggtcggctc atcgagcatc
     9421 aacaggctcg gccgcccaag cagtgctcgg ctgatcccca gcatcttgcg ctcgcctccc
     9481 gaaagtgtac ccgcctgctg ggacaggcgc ttggcgagca agggaaacaa ggtcagcatg
     9541 tccccgatcc ggtccttgct gtcagcagcg cccatggcgc cgatcagaag attttctcgc
     9601 acgctcaatc gcccgaatac cgcttgctcc tgcgggacat aaccgatgcc aagtcgcacg
     9661 cgccgttctg tgggcagtcg cgccacgtcg ctgccgttga aacgtaccac cccacgcgcc
     9721 ggaagcagat cgcccgcgag tgttttcagc agcgtcgtct tgccagcgcc attgcgtccg
     9781 agtattgcca caacaccgcg tgctggcgct ttgagagaca catcgaacag cacctggctg
     9841 gtaccataaa acgtgcagac cccattcgca tcgagcaaag gcgcattcag gtgtggcact
     9901 tcaggcatgg ctgagatatg cctctttgac cttgtcgctg ttctgcactt gcgtggtcgg
     9961 gccgcttgcc acgacctgtc cctgatcaag taccgtgagc gaatcacaga ggtccttgat
    10021 gaaatcaaga tcatgctcga cgattagcag cgagcagatc tgtttgatcg gcgcaagtaa
    10081 ttcgccggtg gcacggcgct cctgactgct caacccggct gtgggttcat cgagcaacag
    10141 cagtttcggc ttgccggcaa gcgccatcgc aatctccagc cattgctgct cgccatgcga
    10201 cagcacgccg gccagatcat cttttctcgc ggacaggcgg aactgctcca gcgttctgat
    10261 cacctgcttg tcgagcgcgc ggcgcgaatg agagaacaac agcctggcga ggctgtcgtg
    10321 ggcctgcacc gccagtaaga tattgtcgta cacgctcaat tgcggtagca cgctggcaat
    10381 ctggaatttg atactcaatc ctgcacgcgc tcgctccgac ggggccgcga acccgatgtc
    10441 gtcgcccaga aagccgattg tgcccgcatc cggcatttcg gcaccggcga tgcatttcag
    10501 cagcgtgctc ttgcccgagc cattgggccc gatcaggccg tgaaattcct tgtcgcgaac
    10561 ggtcaaatcg acaccattga gtgcagtcag ggcgccgtaa attttagtga cattgcgtgc
    10621 ttcgagcaga ttcatgctgt cacgccggtt tctggtccgc gtcgttctgc ggcagcgctg
    10681 cttcatctgg ccgaccgaaa gagccgatcc gttctcggtc cgaagcaaaa aaactgatga
    10741 gcccggtagg gcgaaacatg acgatgatca gcagcagcaa gccaagaatg atcggccaca
    10801 gccgctggta ttgctgggac agcatgaagc tcaggtactc aatcatgccg gcacccgata
    10861 cggcgccgat cagggtgccc acgccgccga acaagacata caacaccacc tgggtggaaa
    10921 gcaccggact cagctgacca gggccgacat aaccttcatg gaatgcatac atgccgccgg
    10981 ccagcccggc gatgaatccg ccaagcgcga aaataatgcc ctttacatgc tgaactcggt
    11041 agccgaaaaa agtgatgcgt gtctcgtgct gccgaatgcc cgcgagaaca agcccgagct
    11101 gcgagcgcgt cagaaatttg agcgcgacat agctaatcag cagtacgccg agtgccatgt
    11161 aataaaacga tacgccttcc gcgatttcat ggccggccag cgtcaatcgc ggcagcgacg
    11221 ggatgccatt ttggccgccc acgtaagacc agccacgcgc aagccgctcc accacgtaag
    11281 agccagtaag cgttccaagc gcgacgaata ccgtgcttgg tacgcggcgt cccaggatga
    11341 caatgaaggc caggatcagc gaaaccacaa ggccgaccag tcccgcgacg ggcagcaata
    11401 ccagtactga agtaatgccc ccttccgtgg cgagcagcgc cgtgcagtat cccgccatgc
    11461 cgaaaaacaa tgcctggcca aagctcagga tgccggcata accccagacc aggtcgaagc
    11521 tcagcgcgag cagcccgagg atgaacacac gcgtgcagaa cagcgtccag aaatcatcgg
    11581 caaaaacagg agcgatcaaa ctggcggcca gcagaatgag ctcgagccac ggaatgatct
    11641 ttcgcagcgg tcgcggcgca atcgacggtt tggtcggatg gctcatgttc cagcaaattg
    11701 cctgtcagag aacgaagggc aaaaacgtga ctcccggcca cctcgcctga agtcacgtcg
    11761 attcaacgaa cacaatcgtc aggcgccgca ctctccgggc atgacggggc ctttgctctt
    11821 ttcgacgatc tcatacttac cgcctttgga taccgccgtg tacatgttca tcttcacgtg
    11881 ccgcgtcccc ggcaccattt cgcaaccacc gccgggcccg tcgctgatct ttgcgtgatc
    11941 cagcgctttg gccacaggct cgcgcttgac gctcttcgct tctttcaccg cggcttccca
    12001 aagcctgatg ccgcgataca tgccagtcgc gccgctgccg gcagtgaacg gattcttgtc
    12061 accgaattgt ttgccgtact cggcctgcaa tttgttgccg aaaggatcgt ttacggcttt
    12121 gaaataatcc aggcagcttg caagcccttc gatctcgtgc gccggcgtaa tacccagcgc
    12181 attctcgtcg tagtacacac aggacaggcg cccgcccttc ttctggaagc cagcctcgtt
    12241 gagctgcttg aaaaaagccg caaccccggg cgggatggtt gtattgaata cgacgttgac
    12301 cccgctcgac atgatcttgt tgaccgtcgc gctgtattca ttgtggtcca ccggaaaata
    12361 ttcttccccg accacttcac caccgttctt ggtgattacc gtgcgtgcat aggcattcag
    12421 ggtgtgcggc cagacatagt cggccgatgg cagatagaat ttcttgcctc cattcttgat
    12481 cagccatgga atgaaagtgt cgcattgctg cgccggcgtt ggcccggtgc agaacagaaa
    12541 aggcgtacat tccttgcctt cgtacagttg aggatagatg tagagcgtct tgccgcgatt
    12601 gacgatggtg tccttgatgg cgttgcgcat ggaactcgtg atgccgccga acacaacgtc
    12661 tactttgtcc tgcgtcacca gcttgcggac cttcgtcacg gcaagctgtt cgttggtggc
    12721 cgtgtcctcg agtatcattt gcagcggccg gcctagtaag cccccgccct ggtttatgtc
    12781 atcgatgacc attttggcga tctgcgaatt gggtataccg ccccagctga ttgcaccggt
    12841 gaggtcggtg gcgatgccga ctttgatcgg atcggctgcc cagacgcctt tagggttgta
    12901 cagatagctt cctccgccca acgcaaccat tcccgttagc agactggtcc gctgcaaaaa
    12961 cagtcttctg gacgcggaca gcggacggtt gcggcattga tcgtttttca ttgttcactt
    13021 cctccacttt gatagaaggc cttccggccg aaccttcacg atcgccaccg cgataatgaa
    13081 gaccacaaca tctgctacga caggtttgat tgcccacggc agtgcggcgc tggtggcgcc
    13141 caccagggcc gcgcctgcaa tagcaccctc gaaacttccc atgccgccga gcatgacggc
    13201 cagaaaagac ttgatcagga acggtacgcc catgtccgcg ctgatggcgt agatcggcac
    13261 catcaaaccg ccggcgaggc cggccagcgc cgccccgaac gcgaatgttg cggcatatac
    13321 acgtcgcgtg gagatgccgg atgcgcgcgc cagtgcggga ttttcaagcg aggcgcgtac
    13381 ttgcaatccg aaacgggtgc gggtaagcaa aagatagcac gcaaccatga ctgcggcggt
    13441 tatgacgatc accgccgtgc gccactggga aatcgagatt tcaccaaatg agaatgaacc
    13501 gaccagcggc gcggtgacag aataaaactg gccgttcaac agcgagcgga tcgtctcgcg
    13561 caatgccaac ccaatggcat aagtgcccaa catggcgacg atcggggtgg cataaaaacg
    13621 gcggatgact gtgcgctcca gcaccaatcc cgcgattccc acggcaatcg gcgcgacggc
    13681 catacccgcc caaacaggca ggcctctgct ctggaacaga taaacggtgt atgcaccgag
    13741 caaaacaaat tcgccatgcg cgaaattgaa aatgcccatc atgctggcta taacgcccag
    13801 g
//
LOCUS       DATMZQ010000026         7024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2780338, whole genome shotgun sequence.
ACCESSION   DATMZQ010000026 DATMZQ010000000
VERSION     DATMZQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780338"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..3165)
                     /locus_tag="VN496_00910"
     CDS             complement(169..3165)
                     /locus_tag="VN496_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15528.1"
                     /translation="MEKKTIYSKTGKGVLEIKNKAGKLAKDLVKILTLIDGKSTVADL
                     ISKSKFSDAEMNKALSDLATAGYIKEFANTSTGTKSGGAGSYVDDLDFTSSLSPGKNV
                     YQNAQSEWRQRETADRAKAEEEAKQKRQQEDEQKKQEAVKLAREESARLAKIEAERKV
                     KEAAAQKLREEAERKEKVQAEAMAQTQRDLSKKLEAERQAFELAERKKLEQQHVQTSE
                     NAARRARQEAEQRVKEEQERRQGQEESRRKEDDRKQAEDDLRRRENDEQQRREEEDRR
                     LREVEERKKHEDEERRQREEDDRKRQEDEARKRREEDELRKREEQRREEERRAEEERK
                     RRDEEQRRHKEEERRRKEEEERVRAEEERKRKDEEARKAREEEEKRLRREEEDRRRRE
                     EDERRRREELDARERRDREEAERARRDEDERREREQQAASSKDDLPEFDLSGLRAMES
                     QVAAEFEKQQEALRKREEEEERVREQEEQARLAVERAERQEQAREEAARREAEDLERR
                     ERMEVERKDREGRERKRQEEKESRVREQEEAKIKREQERERQDAMKVENERRSREDAL
                     VKRRKEQEDRDRKRAEVETLKKQKGIKTPLDRFKPIFIGLVVVAAIIVGGVQVVPMNA
                     YVPSIEKLASTHLKEPVSIGSMHVSILGGLEIILQNVKIGTTQDVKIDKVSLSPEFGS
                     VFGDVKVIHKMQLDSASVAEEVLPRLPKWLDSAVADKALQIERVIAKSIKLESRTAKL
                     PTFDADLTFSPEGSIQKALLAATDGKLNVELVPHDNQVDIALNATKGWVPPLGPQIEF
                     TDLTLKAVANHQRITVEKFESLLYGGAAKGSAVIVWGGPWSIEGEVTTERIELQHLMA
                     AFTRDARSTGQLESNLRYALSSQTVATLFDGPRIEGSFAVKKGDVDGVDLVRALQSGG
                     RGITQGGATRFEEITGTLSLAGGRYQYRNMKLASGLLSATGAFDVSPSKNVSGRITVE
                     LRSQAAQIKGNFVVDGDLKAVVLRPN"
     gene            3541..5310
                     /gene="mutL"
                     /locus_tag="VN496_00915"
     CDS             3541..5310
                     /gene="mutL"
                     /locus_tag="VN496_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXF15529.1"
                     /translation="MPSIHLLPDLLITQIAAGEVVDRPASALKELLENSLDAGASEIS
                     TQLTDGGIRQIRVSDNGSGIAEQDLVLALERHATSKIASLEDLECVASLGFRGEALAS
                     IAAVSHFTLASRAAGSARAWKVEARGGTRTAPEPASIAAGSTVEASELYFNTPARRKF
                     LRTPQTEYAHSEEAFKRIALSRPDVRFMLTHNGRAQWHLQASNEHDRVRAVLGDEFAS
                     ASLPVDVTSAGFHLHGLVAQPAYSRSSRDAQYFFVNGRFVRDKVLSHAVREAFHDVLH
                     HDRHSAYVLFLDLDPALVDANVHPSKIEVRFRDSRGIHQFVFHALEKALSATHAGPNA
                     GHHSVPSAAFRFDGGFVRQSDINLLTNEPASFYATLFGASAGTTALSASSAQSFATLP
                     RDADHPLGFALGQLSGVYVLAQNERGLVVVDMHAAHERIVYEKLKLALEGDGIPTQHL
                     LIPATLNASALEIATAAENPETLSRLGFELAIIAPTVLAVRSVPMSLMQADAAQLARD
                     VLAGIAEFGGTRVLAERQNEMLSTMACHSAVRANRALTLTEINALLREMETTERSGQC
                     NHGRPTWFQITMGELDRMFMRGK"
     gene            5307..6257
                     /gene="miaA"
                     /locus_tag="VN496_00920"
     CDS             5307..6257
                     /gene="miaA"
                     /locus_tag="VN496_00920"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXF15530.1"
                     /translation="MTGGDPEASPAILLMGPTASGKTGVALQVARRFPVEIVSVDSAL
                     VYRDMNIGTAKPDIETRRQVRHHLIDIIDPSQTYSAAQFRDNALAIMRDIVARGRVPL
                     LTGGTMLYFKALRDGLSVLPGADENARKVIDAMAAKAGWAAIHRELERIDPVTAARLD
                     RNDAQRIQRAMEIFYLTGQPMSQLIASQNAVTLPYRMLPIALVPGDRSVLHQRIAQRF
                     ERMLEMGLIDEVRELRKTYVLNPALPSMRCVGYRQTWQFLEDEYGLASLREKAIAATR
                     QLAKRQLTWLRAMDGLTQFDCLATDLQAQIEAWLSRQLQW"
     gene            complement(6295..6699)
                     /locus_tag="VN496_00925"
     CDS             complement(6295..6699)
                     /locus_tag="VN496_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXF15531.1"
                     /translation="MADKKVLTLEGAKKVAAAAEAEAKKNNWNVVIAVVDDGGNLLYL
                     QRIDGTQTGSIEVAIQKARTAQAFKRPTKVFEDAIAGGRNALIALHGALPLEGGLPIM
                     VGGQLVGAIGVSGVKSTEDGQIAKAGADSLSN"
BASE COUNT         1261 a         2213 c         1882 g         1668 t
ORIGIN      
        1 gggttcggcc gtcgctactt agatgaactg cagaaactca ttggggacga aggggcgcgg
       61 tcagtcatcg ggcgtcatcc cgatgacgtg gaacttgttg agtcggatga caccggaatt
      121 ttacgggaca tcgatacgca ggccgacctg cagaggatca gttgatgatc agttcggccg
      181 gaggacaacc gccttcaggt cgccgtccac aacgaagttt ccctttatct gcgcggcttg
      241 cgaccgtagt tccaccgtta ttctgccgct gacgttcttg cttggtgaaa cgtcgaatgc
      301 accagtcgcc gacaatagtc ctgaagcgag cttcatgttg cggtactgat agcgccctcc
      361 cgccagactg agcgtcccgg taatttcttc gaagcgggta gctccgccct gcgtgatgcc
      421 acgtccgccg gactgcaggg cgcgcacaag atccacgccg tcgacatcac ctttcttcac
      481 agcaaagctg ccctcaatcc tgggtccatc gaacagcgtt gccactgtct gggaagacag
      541 tgcataacgc aagttggatt ccagttgtcc ggtcgatctg gcatcccgag tgaatgccgc
      601 catgaggtgc tgcagttcga tgcgctcagt ggtgacttca ccttcaatgg accacgggcc
      661 accccatacg attactgcac tgcccttggc ggctccgccg taaagcagcg attcgaactt
      721 ttcgaccgta atgcgctggt gattggctac cgccttaagc gtcaggtcgg tgaattcaat
      781 ttgcggtccc agcggtggta cccagccctt tgttgcgttc agcgcgatat caacctgatt
      841 atcgtgcggc acgagttcca cgttcagctt gccgtcggtg gcagctagca aagccttctg
      901 tatcgaaccc tcgggcgaaa aagtcaaatc ggcgtcaaag gtcggcaatt tggcggtgcg
      961 ggattcgagc ttgatcgatt ttgcgataac cctttcaatc tgtaatgcct tgtcagcaac
     1021 agcagaatcc agccatttcg gcaatcgcgg caatacttct tccgcgaccg atgcggaatc
     1081 cagttgcatc ttatggatta cctttacatc accgaatacc gatccgaatt ccggagacag
     1141 cgagaccttg tcgattttca catcctgcgt cgtaccgatc ttgacgttct gaagaatgat
     1201 ttccagccca cccaggatgg atacatgcat cgatccaatt gataccggct ccttcaggtg
     1261 cgtagaggcc aatttctcta tggatggtac atacgcgttc atcggcacga cttgcacgcc
     1321 gccaacaata attgccgcaa ccacaaccag cccgataaaa atgggcttga aacggtccag
     1381 gggtgttttg attccctttt gcttcttcag cgtctcaact tcggcacgct tgcggtcgcg
     1441 gtcctcctgc tctttgcggc gctttaccag agcgtcctca cgcgagcgcc gctcattctc
     1501 gaccttcatc gcgtcctggc gctcgcgctc ctgttcgcgc ttgatcttcg cctcttcctg
     1561 ctcccgaacc cgggattcct tctcttcctg gcgcttgcgc tcccttccct cacggtcctt
     1621 gcgctccacc tccatgcgtt cgcgtcgctc aagatcctct gcttcgcgcc gggcggcttc
     1681 ttcacgcgcc tgctcttggc gctctgctcg ttcaacggca agccgggcct gttcttcctg
     1741 ctcgcgaacg cgctcttctt cttcttcgcg cttgcgcaac gcctcctgct gtttctcgaa
     1801 ttccgccgcg acctgtgact ccatggccct cagaccggac agatcaaatt cgggcaggtc
     1861 gtcttttgac gaagccgcct gttgctcacg ctcccgccgc tcgtcctcgt cgcggcgcgc
     1921 gcgttccgct tcttcgcggt cacggcgctc acgggcatcc agttcttcgc ggcgccggcg
     1981 ctcatcctcc tcgcggcgcc ggcggtcttc ttcttcacgc cgcaagcgct tctcttcttc
     2041 ctcgcgcgct ttgcgcgctt cctcatcctt gcgttttcgt tcttcttccg cgcgtactcg
     2101 ttcctcttct tccttgcgcc tgcgctcttc ttccttgtgc ctgcgctgtt cctcgtctcg
     2161 tcgtttgcgc tcttcctccg ctcggcgttc ctcttccctg cgctgttcct cgcgcttcct
     2221 gagttcgtct tcctctcgtc gcttgcgtgc ctcatcttcc tgccgcttgc ggtcgtcttc
     2281 ctcccgctgc cgtcgctctt cgtcctcgtg ctttttgcgc tcttcaactt ccctgagccg
     2341 gcgatcttcc tcttccctac gttgctgctc atcgttctca cggcggcgca ggtcatcttc
     2401 agcctgcttg cggtcatcct ccttgcgcct gctttcttct tgtccctgcc tgcgctcttg
     2461 ttcttccttg actctttgct cggcttcctg gcgagcacgg cgcgctgcat tttccgaggt
     2521 ctgcacgtgc tgctgctcaa gtttcttgcg ttcggccagt tcaaaagcct gccgctcggc
     2581 ctcgagcttt ttggaaaggt cgcgctgcgt ctgcgccatg gcctctgcct ggactttctc
     2641 cttgcgctcc gcttcttcgc gcaatttttg cgcggccgct tccttcacct ttcgttctgc
     2701 ctcgattttt gccagccggg cgctttcttc ccgagccagc ttcactgctt cctgtttctt
     2761 ctgctcgtct tcctgctgcc gcttttgctt ggcttcttct tccgccttgg cacggtcagc
     2821 ggtctcccgt tggcgccatt cgctctgcgc attctggtag acgtttttgc ctggtgaaag
     2881 agaagaagtg aagtccaggt catcgacgta ggaacctgca ccgccacttt tggtgccagt
     2941 ggaagtattc gcaaattcct tgatgtaacc ggcggttgcc agatcgctca gcgctttgtt
     3001 catttcggca tcgctgaact ttgacttgga gatgagatct gcgacggtcg atttgccgtc
     3061 gatgagagtg agaatcttga ccagatcttt tgcgagcttg ccggctttgt tcttgatttc
     3121 cagcacgcct ttgccggtct tcgagtagat cgtcttcttt tccatcctgt tctttctcag
     3181 ctcccggaaa gcgcaacgct gcaaccgact tccgcatgat tgccccgcct gccggatgca
     3241 gacgggaatg aaataaacaa accaagcctc gtcgcaaaag cgttgaaaac tttctaatta
     3301 ttaggcttgg cgcggcgcac gtctagggcc gggtggggat aaatagaatt atgatgcctt
     3361 gaaatgtcgc cggatgactg attcaacgct gacttttaaa tttttttgga gtgcccgcgt
     3421 cagtgcacaa agcaacgcct gcgtttccac aacaccacgg acgcgctcgc ggcaacttgt
     3481 ggcgccaaga ctgaaagcgt tcacgtgcag ctataatcga acccgaactg ctatctttcg
     3541 atgccttcca tccatctgct cccggatctg ttgataaccc aaatcgccgc aggcgaagtg
     3601 gtcgacaggc cagcgtccgc cctgaaggaa ctgctggaga acagcctcga cgcgggcgcc
     3661 tctgaaattt ccacgcaatt gacggacggt ggcattcggc aaatacgtgt gtcggataat
     3721 ggttccggta tcgccgaaca ggatcttgtc ctcgcgctcg agcgtcatgc caccagcaag
     3781 atcgcctccc tggaagacct ggaatgcgta gcaagcctcg gctttcgcgg cgaggcgctg
     3841 gccagtatcg cagccgtttc gcattttacc cttgccagcc gggcggccgg cagcgcgcgc
     3901 gcatggaaag ttgaagcgcg tggcggcacg cgcacggcac cagaacccgc ctcgattgcg
     3961 gccggttcta ccgtcgaggc ttccgagctt tacttcaata cgccggcccg gcgcaaattt
     4021 ttgcgcaccc cgcaaacgga atacgcgcat agcgaggagg ccttcaagcg gatcgcgctg
     4081 tcgcgtccag acgtgcgttt tatgctgact cacaacgggc gcgcgcaatg gcaccttcag
     4141 gccagcaacg agcacgatcg cgttcgtgcg gtgctggggg acgaattcgc ctctgcctct
     4201 ctgcccgtcg acgtcaccag cgcgggcttc cacttgcacg ggctggtggc gcaaccggcc
     4261 tactcccgca gctcgcgcga tgcgcagtat ttttttgtca acggacgatt cgtccgcgac
     4321 aaggtccttt cacatgcggt gcgcgaagcg tttcatgatg tgttgcatca cgaccggcat
     4381 tcggcttacg tcctgttttt ggatctcgat cccgccctgg tggacgccaa tgttcatcca
     4441 agcaagatag aagtacgttt ccgtgattcg cgtggcatac accagtttgt ttttcacgcg
     4501 ctggaaaaag ctttgtcggc cacgcatgcc gggccgaatg cgggtcacca ttcggtccct
     4561 tccgccgcgt ttcgcttcga tggcggcttt gtgcgtcagt ccgacatcaa cctgctgacc
     4621 aatgaaccag cttccttcta tgccacgcta tttggtgcgt ccgcgggcac aacggcgctt
     4681 tccgcatcgt ctgcgcagtc gtttgccacg ttgccccgag atgcggacca tccattggga
     4741 tttgcgctcg gacagctgtc cggggtctat gtgctcgcgc aaaacgagcg tggcttggtc
     4801 gtggtcgaca tgcatgccgc gcacgagcgt atcgtttatg aaaagctcaa gcttgcgctc
     4861 gagggtgacg gcatcccgac ccagcatttg ctgattccag cgacgctgaa cgcaagcgct
     4921 ttggagatcg ccaccgccgc cgaaaatccg gagacgctgt cgcgtctggg cttcgaactg
     4981 gccatcattg ccccgaccgt gctcgcagta cgctccgtgc cgatgagtct tatgcaagcc
     5041 gatgcggcgc agctggcgcg cgacgtgctc gccggaatcg cggaatttgg cggtacgcgc
     5101 gtgcttgcag aacggcagaa tgaaatgctg tcgaccatgg cctgccactc agccgtcagg
     5161 gccaatcgcg ctctgacgct aacagagatc aacgcgctgc tgcgcgagat ggagacgacc
     5221 gaacgttccg ggcagtgcaa tcacggccgg ccgacctggt tccagatcac gatgggtgaa
     5281 ctggacagga tgttcatgag aggaaaatga cgggcggcga cccagaggcg tccccggcga
     5341 tcctgctgat gggccccacc gccagcggaa aaactggcgt cgcgttgcaa gtcgcgagac
     5401 ggttcccggt tgaaatcgtc agcgtggatt cggcactcgt gtaccgggac atgaacatcg
     5461 gtaccgccaa accggacatc gaaacgcgcc ggcaggtccg ccatcatttg atcgacatca
     5521 tcgatccctc gcagacatat tcggcagctc aattccgcga caacgcgctt gcaatcatgc
     5581 gggacatcgt ggcacgcggc cgcgtaccgc tattgactgg cggcacgatg ctgtatttca
     5641 aagcgctgcg tgatgggttg tccgtactgc cgggggccga cgaaaacgcg cgcaaggtga
     5701 ttgacgcaat ggccgccaaa gccggctggg cggccattca ccgcgaactc gagcgcatcg
     5761 accccgtcac cgcggcaaga ttggaccgca acgatgcaca gcgcatccag cgggctatgg
     5821 aaatcttcta tctgactgga caaccgatgt cgcaactgat cgcgtcacaa aatgcagtca
     5881 cgttgcctta ccggatgctc cccatcgcgc tggttccggg cgatcgcagc gtcctgcatc
     5941 agcgcatagc gcaacgcttc gagcgcatgc tggaaatggg cctcatcgac gaagtacgcg
     6001 aattgcggaa gacctacgtc ctcaatccgg cgcttccatc aatgcgctgc gtaggatacc
     6061 ggcagacgtg gcaatttctc gaggatgaat atggattggc aagtttgcgc gaaaaggcaa
     6121 tcgctgccac gcgccagctc gcgaaacgac agttgacgtg gcttcgcgca atggatgggc
     6181 tcacacaatt cgattgcctg gccacagacc tgcaggccca gatcgaagcc tggctgtcgc
     6241 ggcagttgca gtggtgaggg agcgccgcta tgcgctcccc cgtacgcagc ccgttcagtt
     6301 actcaaagag tcggctccgg ccttcgcgat ttggccgtcc tcagttgatt tgacgccgct
     6361 gacgccaata gcgccgacca actgtccgcc gaccataatt ggcagcccgc cctccaacgg
     6421 caaagcgccg tgcaatgcga tcagcgcgtt gcggccgccg gcaatcgcat cttcaaacac
     6481 tttggtggga cgcttgaaag cctgcgcagt gcgcgctttc tggatggcga cttcaatgct
     6541 tcccgtttgc gtgccatcga tgcgctgcag gtaaagcagg tttccgccat cgtccaccac
     6601 ggcgatcacc acgttccagt tatttttctt tgcctccgcc tcggccgcag ccgcgacctt
     6661 cttggcaccc tcaagcgtca aaaccttttt gtccgccaat tgcgcagcag cgctgaagga
     6721 cagcaagccg aacactacga acaagcctaa agaaatcaat ttttttgcca aaagcatggt
     6781 atctcctcgg atggtcaatt ttttcggacc ggatcggatg cccggtgaac aggcagtcaa
     6841 caagcagacg tgcactggat acgataaccc acccgacttc caaatccaaa tcagtcttct
     6901 ggaatatcga tatgctcgac acgcgagcgc gccgccccgc tggcaaacgt caaacgcagc
     6961 aactcaccag cggcaatctg tttggcggcg ctcacgattc gtccgtcatt tttctgcaca
     7021 atgc
//
LOCUS       DATMZQ010000027         6256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1391241, whole genome shotgun sequence.
ACCESSION   DATMZQ010000027 DATMZQ010000000
VERSION     DATMZQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391241"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VN496_00930"
     CDS             complement(<1..251)
                     /locus_tag="VN496_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15532.1"
                     /translation="MAYQVVRKPSELFFPVSDALYKTPPETWKAYGPLFRQHATEVMT
                     PALLAALAQVEGSGNPVARTYWRWSLQRRPFEVYRPASSA"
     gene            710..1507
                     /locus_tag="VN496_00935"
     CDS             710..1507
                     /locus_tag="VN496_00935"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HXF15533.1"
                     /translation="MKRLPPEKKRDVTQRTSEKRALAKTVTSMQERRHNILNRVNLAP
                     NPAITTEPVLQVYGARCRGWRGYFSLHTWIAVKPAAAKAYTVYEVTPETLVRWGRCVS
                     IRKRVPDAHWYGAAPELLADRRGEHVGPLIERIRNAAQDYRYANKYLMWPGPNSNTFI
                     AHLARVVPELELELPCHAIGKDYLGLKLIGRPASGSGCQLSFFGLFGILVSRVEGIEL
                     NLLGLSCGFHPFAEYVKLPFVGHIRALRMTTEEQPPTVPAGAAEIAE"
     gene            complement(1549..1890)
                     /locus_tag="VN496_00940"
     CDS             complement(1549..1890)
                     /locus_tag="VN496_00940"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HXF15534.1"
                     /translation="MITIQHEGNLSVIGIFATFEIEDFRRLETEVEKQLRDLGQIDLL
                     VDLRAMLGYTLDAALEDIRFSREHALDVGRIAILSEDDAVVWTALLSRIFVRAEIQVF
                     DNEALAREWLE"
     gene            complement(1985..3301)
                     /locus_tag="VN496_00945"
     CDS             complement(1985..3301)
                     /locus_tag="VN496_00945"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXF15535.1"
                     /translation="MSIAAISLLMLAAFLLLIIVLGMPIAFATGLIAVVFCAAFADLD
                     SLAIVTFRTYGFVNSYVLLSVPMFLMMASVLDKSQVAHDLYDALKIWAGRVPGGIGVM
                     TLIAASVMAAMTGIIGGEVILLGLVALPQMLRLRYDSKLAVGIVCAGGSLGTMVPPSL
                     VLVFYGLTTSTSIGDLFIASTIPGIMLALIYIAYVLIRCGFNPSLGPPAPPEIRNIPL
                     RAKFAALKKVLLPLAIIFSVLGSIYTGLTSVSEAAAMGVAGVIIAAAIRRTLNWKMLR
                     DSLYTTMSTCGLLIWLSIGANAMVGVYNLLGGITYLKSIMTGLPLDPIGVILIMMLIF
                     LVLGMFMDWFSIMLLTMPIFAPTVTALGYDLIWFGVLFNITMQVGYLSPPFGQAAFYL
                     KSVAPPEISLNHIFVSMLPFIGLQIIGLMVVLFVPDVAMVLIRLLH"
     gene            complement(3298..3822)
                     /locus_tag="VN496_00950"
     CDS             complement(3298..3822)
                     /locus_tag="VN496_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXF15536.1"
                     /translation="MEERASQASPPRNAVDRAIEAIAKLLSYSFALSIVVTIYDVISD
                     VIFRAPTDWVYDVVTTAIAVSFLIGGSYALMRREHIRISAVYDRYSRRTRLRCDIVTS
                     LLAIIYLIAFGWFAYNMASLSVADWEVGGSAWAQPTPVVVKVSMVLGALLMVVQMISN
                     IRGDVQSLRAERQL"
     gene            complement(3902..4921)
                     /gene="dctP"
                     /locus_tag="VN496_00955"
     CDS             complement(3902..4921)
                     /gene="dctP"
                     /locus_tag="VN496_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXF15537.1"
                     /translation="MLRTLKLVNVCAAFALMAGLTQPGTVGATEHKWKVQNLYGPNTT
                     VYKDFQAFTARVKEATKGEVDIQAFPVATIVPQAEALDAIKAGLLDGAVGTMAYQGGK
                     EPAAAFWDMTAAYDNPLHLLMWYRQGGGMEIMNKISAKWDAVFLGPVILGLESIPSKK
                     PIRSIADFKGVKMRAPDGIPAEIFAKLGASVSVMPGTEVYTALDTGKIEATDWGTLSV
                     NDEAGYNRIAPYAIYPGIHSMNSVDFVVSKRKWNALSAEQKAVVTAAVDEWCLRTWMD
                     QELEDRRAVAKRDPKTLIPWGEKEKAAFRKVSQSVWEKWSKKSPLAKEIYESQLNFLK
                     SIGKL"
     gene            complement(5043..5222)
                     /locus_tag="VN496_00960"
     CDS             complement(5043..5222)
                     /locus_tag="VN496_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15538.1"
                     /translation="MQLQRPEPPVHDPSIVPPAIVPPSIDEPPPVKIPPEEEPPILPP
                     DDDPGPVHEPPPRKH"
     gene            5590..5759
                     /locus_tag="VN496_00965"
                     /pseudo
     CDS             5590..5759
                     /locus_tag="VN496_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142829.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
     gene            complement(5831..>6256)
                     /locus_tag="VN496_00970"
     CDS             complement(5831..>6256)
                     /locus_tag="VN496_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXF15539.1"
                     /translation="ASGDLAGTPSLDEDYLVLSTIHSAKGQEWDAVHVLNIADGNFPL
                     EFATGKADQIEEERRLLYVAMTRARTDLHLIAPLRYYVTQQSRRGDDHVYGAQSRFMT
                     HAVLAQCEQITWPRSDVGASHSSANAPKVDVAGMLRGMW"
BASE COUNT         1395 a         1826 c         1779 g         1256 t
ORIGIN      
        1 gcgcttgagg ctggccgata gacctcaaaa ggcctgcgct gcaaagacca gcgccagtaa
       61 gtgcgcgcga caggattgcc cgacccttcc acctgcgcca gggcggccag aagagcaggc
      121 gtcatgactt cggtcgcgtg ctgtcgaaac agggggccgt aagccttcca ggtttctggc
      181 ggcgtcttgt aaagcgcgtc gctcaccggg aaaaacagtt ctgaaggctt gcgcacaacc
      241 tggtaagcca agttgaccaa gaggaccgca atgcaggaag ccgccagcag aaggagaaat
      301 ttgataccgg aaggcgcgtt gcgcaatttg cgccaccatc ttcgacgcag ctgtgcacgc
      361 ggccagaagg gcatcacgcc gtaacggcgc ctgcgctttt ttcgtggacg ggaagttgcg
      421 gttcgagtta aagatggcgt ttgcccgctc gcacttgtcg tgctactcca aacagatttc
      481 atcaccccgc attatgcgtt aagcgacggg cagcgacgaa tgttcgcgcc aatcgcccga
      541 gagctcattg ccgccgattg ttacgtgaag ttggatgcca tctctaggcc gaaggagcca
      601 cgcgcattga cgattccgag cgtggttgat gcttatgcat gctcatatca tcgaaccaat
      661 gcgacagaga aagggattgt cgatgcgctt ttttcactgg ctaacaccct tgaaacgcct
      721 cccaccggag aagaaacggg acgttacaca acggaccagc gaaaaacgcg cgctcgcgaa
      781 aactgtgaca agcatgcagg agcgccggca caatatactg aatcgggtaa atcttgcacc
      841 caatcctgca ataactaccg aaccagtcct ccaggtttac ggagcccgct gccgtggctg
      901 gcgcggttac ttcagcttgc acacctggat cgcggttaaa cccgctgccg caaaggcgta
      961 cacggtatac gaagtcacgc cagagaccct ggtgcgctgg ggacgctgtg tcagcatacg
     1021 caagcgggtg cctgacgccc actggtacgg ggcagcaccg gaactattgg cggataggcg
     1081 cggcgagcac gtaggaccac tgattgagcg catccgaaat gccgcacaag actatcgcta
     1141 cgccaacaaa tatctgatgt ggcccgggcc taattccaac acgttcatcg cgcatctcgc
     1201 ccgcgtcgtg cctgaactgg aactcgaact gccctgccat gccattggca aggattatct
     1261 gggattgaaa ttaatcggca ggccggccag tggcagcggc tgtcaattat cgttttttgg
     1321 actgttcggc atcctggtca gccgcgtgga agggattgaa ttaaatttgc tgggacttag
     1381 ttgcgggttc cacccgtttg ccgaatacgt aaagctgcca ttcgttggtc acatccgcgc
     1441 tttacgcatg acaactgagg aacagccgcc taccgtcccc gctggcgcag ccgagatagc
     1501 agagtaaatg cgcctcgaag aagcggcgct gttccgatcc gcaggttgct attccagcca
     1561 ttccctggcg agcgcttcat tgtcgaaaac ctggatttcg gcccggacaa aaatcctgga
     1621 cagcagcgct gtccacacca ccgcatcgtc ttcactgagt atggcgatac gtcccacatc
     1681 cagcgcgtgc tcgcgactga atcgaatgtc ttcgagcgca gcgtccaggg tatagcccag
     1741 cattgcccgc aagtccacca gcagatcgat ctgtcccaag tcgcgcaatt gcttctcgac
     1801 ttcggtttcc agtcgccgga aatcttcgat ctcgaaggtc gcgaatattc cgatgacgct
     1861 gagattgcct tcatgctgaa tggtaatcat cgctttggtt taagactcac ctttcggatt
     1921 gacatgcagc gtgacataag ttggatggcg ccaatgatcg caatcacccg gtgaccgccg
     1981 tacgctaatg cagcaaccgg atcagcacca ttgcgacatc cgggacgaac aacaccacca
     2041 taagaccgat aatctgcagc ccgatgaacg gcaacatcga aacaaagatg tgattcaggc
     2101 tgatttcagg cggtgccacg gacttcagat aaaacgcagc ttgcccaaac ggtggcgata
     2161 gataccctac ctgcatggtg atattgaaca gcacaccgaa ccagatcagg tcatagccca
     2221 gcgcagtgac ggtcggagcg aatatgggca tcgtcagcaa catgatcgag aaccagtcca
     2281 tgaacatgcc gaggaccaga aagatcagca tcatgatcaa gatgacgcca atcggatcga
     2341 gtggcaggcc agtcatgatg cttttgagat acgtaatgcc gcccagtaga ttgtagacgc
     2401 ccaccatggc gttagcgccg atggacagcc agatcagcag gccgcaagtc gacatggtgg
     2461 tatacagcga atcgcgcaac attttccagt tcagggtgcg ccggattgcc gccgcgatga
     2521 tcaccccggc gactcccatc gccgccgcct cactgacgga agtcagtccg gtatagatac
     2581 tgcccagcac ggaaaagatg atcgccagcg gcagcagcac tttcttcagc gcggcaaact
     2641 tggctcgcag cgggatattc cggatttcgg gtggggccgg cggcccgaga ctgggattga
     2701 aaccgcagcg gattaataca tacgcgatat agataagcgc cagcatgatg cccggaatcg
     2761 tcgacgcaat gaaaagatcc ccaatggaag tcgatgtcgt aagcccatag aacaccagca
     2821 cgaggctggg gggaaccatg gtgcccagcg agccgccggc gcacacgatg ccgaccgcaa
     2881 gcttggagtc atagcgcagg cgcaacatct gcggcagcgc gaccaggccc agcaggatga
     2941 cttcgccgcc gatgattccg gtcatcgccg ccattacgga cgccgcaatc agcgtcatga
     3001 cgccgatgcc gcccggtacc cggcccgccc agatcttcaa cgcatcgtag agatcgtgcg
     3061 caacctgcga cttgtccagg acagaggcca tcatcagaaa catcggtacc gacagcaaca
     3121 cataggaatt gacgaaaccg taggtgcgaa aggtcacaat ggccaggctg tcgagatcgg
     3181 cgaaagcagc acagaacacc acggcaatga ggccggtggc gaaagcgatt ggcatcccca
     3241 gcacgatgat gaggagcaga aacgccgcca gcatcagcaa tgaaatggcc gcgatgctca
     3301 taactggcgt tcagcgcgca gggactgcac gtccccgcgt atattggaaa tcatttgcac
     3361 gaccatcagc agagcgccga gcaccatgga caccttgaca accaccggtg tgggttgcgc
     3421 ccaggccgag ccgcccactt cccagtcggc aaccgacaac gacgccatgt tgtaagcgaa
     3481 ccagccgaac gctatcagat agatgatggc cagcagagac gtcacgatgt cgcaacgcag
     3541 ccgcgtgcgc cgggagtagc gatcatagac ggccgagatg cgaatgtgtt cgcggcgcat
     3601 cagggcataa gaaccaccga tgaggaacga caccgcgatg gcggtggtga cgacgtcgta
     3661 gacccaatcc gttggtgcac gaaaaatgac gtcgctgatg acatcataga tcgtgactac
     3721 gatcgatagc gcgaaactgt agctcagcag tttggcaatg gcttcgatgg cgcgatcgac
     3781 cgcgttccgt ggcggcgacg cttgtgatgc gcgttcctcc atgtcccctt accgtccagt
     3841 acgcgagcgc aacatcgtcc tcagtcctgc gtcgttgcgt ggtcgcgggt cggagtcggg
     3901 ttcaaagctt gccgatcgac ttcagaaagt tgagctggct ttcgtagatt tccttggcga
     3961 gcggactctt cttcgaccat ttttcccata cggactgcga caccttgcga aacgctgcct
     4021 tttccttttc gccccatggg atcagcgtct tcggatcgcg cttggctaca gccctgcggt
     4081 cttcgagttc ctggtccatc caggtccgca agcaccactc gtccaccgcc gcggtcacga
     4141 cggctttctg ctcggcagac aacgcattcc acttgcgctt gctgactacg aaatcgaccg
     4201 aattcatcga atgaatgccc ggatagatgg cataaggcgc gatgcggttg tagccagctt
     4261 cgtcgttcac cgaaagcgtg ccccaatcgg ttgcttctat cttgccggta tcgagcgccg
     4321 tatagacttc cgtgccgggc atcacggaga ctgaggcgcc gagcttcgcg aaaatttcag
     4381 ccggaatgcc gtccggtgca cgcatcttca cgcctttgaa atccgcaatg gagcggatgg
     4441 gctttttcga tggaatcgat tccagtccga gaatgaccgg gcccaggaat accgcatccc
     4501 atttggcgga aatcttgttc atgatctcca tgccaccacc ctgccggtac cacatcagca
     4561 agtgcaacgg attatcgtaa gcagcagtca tatcccagaa cgcagcggca ggctccttgc
     4621 cgccctgata tgccatcgtg ccaaccgcgc catcaagcag cccggccttg atagcatcaa
     4681 gcgcttcggc ttgcggcact atggtcgcca ccgggaaagc ctggatgtcg acttcccctt
     4741 tggtggcctc cttcacgcgt gccgtgaagg cctggaaatc tttgtaaacg gtcgtattgg
     4801 ggccatacag gttttgcact ttccatttgt gctctgttgc cccgaccgtt ccgggctggg
     4861 tcaaaccagc catcaaagcg aacgctgcac aaacattcac cagcttgagc gtacgaagca
     4921 tagttcctcc tcattttttt tgtggtgcaa cggatctggc gatgggaacc ttaccatact
     4981 gtccagaaag gccgctaaaa cagcttgcaa gtgcagctag cgaaaaagca cagtgcccgt
     5041 acctaatgct tgcgcggcgg cggctcgtgc accggtccgg gatcgtcatc cgggggcagg
     5101 atcggcggtt cttcttccgg gggaatttta actggcggtg gttcgtcgat ggacggcgga
     5161 acgattgcgg gaggcacgat gctcgggtcg tggaccggcg gttctggtct ttgcaattgc
     5221 acggcaacgt cctcccccaa acgggctcgt aactgctgac accatcgcgg ctggaagatt
     5281 catggcaacg tctggtctcg cgcggtgcaa cgcgccgcat gcagggcggg tggctgattc
     5341 ctggagagga aattttctcc atcattggta aattttgcgc cagcgccaat gtcggcggtt
     5401 caggcagcgc cttgtaggat aaccgccagc tagtgtgact acctgctgag aatttcgagc
     5461 acttcttgcg cgtcgttctg gctggcgtcg cgctcgattc gcaacccagc ggttcatgaa
     5521 cgttgtttgg atagacagca atggctacta cgcaaatgta tccagacacg gtggatcggg
     5581 actcatacga tgcgcgcgat cgactggatc gcgatgatcc tgatgattat cggcgccatc
     5641 aactggggtc ttatgggata tcgaacttcg atctggtggc gaccctgttc ggcgcgcatt
     5701 cggggctcac acgactgatc cacgcgctcg tcggcctggc ggcgctatac gggatttaca
     5761 tggtcatctg ctgggggccc ggagtcgcga ttcgcgtacg acctagacca tcagcggaag
     5821 cccgcttcag ttaccacatg cccctcagca ttccagccac gtcgaccttg ggcgcatttg
     5881 ccgaactgtg cgatgcgccc acgtcggagc gcggccaagt tatctgctcg cactgcgcaa
     5941 gcacggcatg ggtcatgaag cggctttgcg ccccgtagac gtggtcgtcg ccgcgtcgcg
     6001 attgctgcgt gacgtaatag cgcagcggcg cgatgaggtg caggtccgtc ctggcgcgcg
     6061 tcattgcgac atagagcaaa cggcgttctt cctcgatctg gtctgccttg ccggtagcaa
     6121 attccaaagg gaagttgccg tctgcaatgt tcagcacgtg tactgcatcc cactcctggc
     6181 ccttcgcgga atggatggtg gagaggacca ggtaatcctc gtccaatgac ggcgtgcccg
     6241 ccagatcacc gctggc
//
LOCUS       DATMZQ010000028         5167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1058740, whole genome shotgun sequence.
ACCESSION   DATMZQ010000028 DATMZQ010000000
VERSION     DATMZQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058740"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VN496_00975"
     CDS             <1..874
                     /locus_tag="VN496_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF15540.1"
                     /translation="LDGTPAVAVAGEADGVRNISPMLTITRTWNSVSAVSYMRRGLAL
                     ARDYAAKRVAFGAPLAGKPLHVDTLAGLQAEYEAGFHLAFFVVELLGRDESGVLDSAG
                     KDLLRLLTPVAKLMTGKQSVSVLSEILESFGGAGYIEDTGLPSLLRDAQVFSIWEGTT
                     NVLSLDVLRALQSGSALAALQVETETLASGVRDMLLLECMKQAQAGLLHAQHWIEKAA
                     GDALEAGARRFSLTLGRSFSLLLLCRHAQWSLDHEHDRRALYAAQRFSRNTVDVIALE
                     DGKASLALANDVEA"
     gene            complement(1220..1537)
                     /locus_tag="VN496_00980"
     CDS             complement(1220..1537)
                     /locus_tag="VN496_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15541.1"
                     /translation="MLKKQVQYEQVMNDAVEHVFVYAVRQAMDTRKLIAQFDAGRGPG
                     PHEEHTLTISLRDTDLSVNAEHIPHEWLSMGTGYIDMRFSKRIAALLQELNQMAQKTG
                     RFI"
     gene            1801..2181
                     /locus_tag="VN496_00985"
     CDS             1801..2181
                     /locus_tag="VN496_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15542.1"
                     /translation="MALSRAVIWIDHHNAQVLQFDAEHVQAQKIKSHTHHTRQHGSSV
                     RTEHEFFGDVCNALGGIAQVMLTGSHTAQSDFRHYVEKHRPSLAKQIVGWETVDHPTD
                     GQLVALARKYFDQFDRRGGPATPA"
     gene            complement(2206..4887)
                     /locus_tag="VN496_00990"
     CDS             complement(2206..4887)
                     /locus_tag="VN496_00990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF15543.1"
                     /translation="MKSTHYLAPLFAPDSVAVIGATERPGAVGSVLMENMLSAKFTGA
                     LYAVNPQRTRVHGVACYPSIADIPQQVDLAVIATPPESVPGVIDACGRAGTRSAVVIT
                     AGFSETGSAGAMLERALLENARRYGMRLLGPNCLGLMRPGIGLNATFAHGNAIAGSLG
                     VVSQSGAICTALLDWARPNNIGFSSVVSLGGSADLDFGEIVDYLTYDTATEQILLYIE
                     GIRNARRFVGALRAAARTKPVIVMKAGRHPTGVRAAVSHTGALVGADDVFQAAVRRTG
                     AVRVATIGQLVAAAQALSAHVEPRGDRIAIVTNGGGPGVLAADHAADLGLPLAQLSAP
                     TVQALSKTLPVNWSHGNPLDLIGDADAGRYETAVSACLADQNVDGVLAILTPQAMTSP
                     VEVARAVVRSATASTKPLLAVWMGEEQVIEGRKVFQTAQRPVFRTPEPAVELFAHLSA
                     FYRNQRMLIQVPGPLSDHHAPDITGARRIIETALASGTSVLSSTDSKAVLAAFHIPVA
                     LSLQARSAQEAIAAAMQCGYPVAMKIDSPDITHKTEVGGVRLDLQNEEAVRSAFDAII
                     DSAQRTRPQARIRGVTVERMLNRSSARELMVGVLTDKVFGPAVTFAAGGTIVEILRDR
                     AVGLPPLNRFLAEEMIGSTRISRLLGAFRGMPPVDLNAVANVLLRVSEMVCELPWIRE
                     LDINPLLADATGASAADARIVIAPCAPGALAYAHLAIHPYPAQLASNWSAKDGTKIVV
                     RPIRPEDAHIEREFVQALSPQTKYLRFLGAMKDLTPAMLARFTQVDYDREMALIAVVE
                     NQGRERQIGVARYVINPDATSCEYAIVVSEDWQGRGLGRYLMLQLIAIARARGLKTMI
                     GLVLSVNTQMLKLAAELGFSVHTPPHDPTSKEVRLAL"
     gene            complement(4962..>5167)
                     /locus_tag="VN496_00995"
     CDS             complement(4962..>5167)
                     /locus_tag="VN496_00995"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858068.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and pyruvate from
                     2-dehydro-3-deoxy-D-galactonate 6-phosphate; functions in
                     galactonate metabolism; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HXF15544.1"
                     /translation="ALRAILPKDVPVLPVGGITPGSMREYWEAGANGFGLGSALYKPG
                     DTPANVAKAAAEFRMALKKVVSG"
BASE COUNT         1027 a         1521 c         1558 g         1061 t
ORIGIN      
        1 tttggacggc acgccggccg ttgccgtagc gggcgaagcc gatggcgtgc gcaatatttc
       61 tcccatgctc accatcacac gcacctggaa cagcgtcagc gcggtgtctt acatgcgccg
      121 cggattggca cttgcacgcg actatgcagc caagcgcgtt gcctttggcg cgccactggc
      181 gggaaaacct ttgcatgtcg atacgctggc gggactgcag gcggaatacg aggcgggttt
      241 tcatctggct ttcttcgtgg tcgagctgct cggacgcgat gaaagcggtg tgcttgactc
      301 agcaggcaaa gacttgttgc gactgctgac gccggttgca aaactgatga ccggaaaaca
      361 atccgtgtca gtgttgtcgg aaatactcga aagcttcggc ggtgcgggct atatcgaaga
      421 cacgggattg ccttcgctgt tgcgcgacgc gcaggtcttt tcgatctggg aaggcactac
      481 aaacgtattg tcgctcgacg tgctgcgtgc cttgcagtcc gggagtgcac ttgcggcgct
      541 gcaggtcgaa accgaaacgc tggcgtccgg cgtgcgcgac atgctgttgc ttgaatgcat
      601 gaaacaggcg caggccggcc tgctacatgc gcagcactgg attgagaagg ccgccggcga
      661 tgcacttgag gcgggggcgc ggcgttttag ccttactctg ggtcgttcct tctcactgct
      721 gctgctatgc cggcacgcgc aatggtcgct cgatcatgaa catgaccgtc gtgcgcttta
      781 tgccgcacag cgtttctcgc ggaacacagt cgacgtcatt gcactggaag acggtaaggc
      841 atcgctggcg ctggccaatg atgtggaagc gtgaaggccg tgaatgccgt gactgccgta
      901 actgccgtga atatcgtgaa tgtcgtgaag tccttctaga gggaatgtct tcaggcgatg
      961 tgtgggtagt tgtggcgtaa ctgccgtgaa tatcgtgaat gtcgtgaatg tcgtgaagtc
     1021 cttctagagg gaatgtcttc aggcgatgtg tgggtagctg tggcgtaatt acggtattca
     1081 ccacatttat gggcgtcagc ccggttatgc gtgcggtacc cctttgggga tccccttgcg
     1141 ggacacggat tcacgtctga cattgtttac gacattcacg gcattcacga cattcacggc
     1201 ctttacggca ttcacggcct taaatgaacc gccccgtctt ttgtgccatc tggttcagct
     1261 cctgcagcag tgcggcaatg cgctttgaga aacgcatatc gatatatccg gtgcccatgg
     1321 aaagccattc atgcgggatg tgttcagcgt tgacggacaa gtctgtatct cgcaggctta
     1381 tcgtcaaggt gtgttcctcg tgggggccgg ggccccggcc tgcgtcgaat tgcgctatca
     1441 gtttgcgcgt gtccatggct tgcctgaccg catagacgaa aacatgttcc acggcatcgt
     1501 tcatgacttg ctcgtactgc acctgcttct ttagcatcgg ctgcacatgt tgctgttgtg
     1561 gacgcctgca caatccattt cgccgcctcc ccatgtcggg tcaggttagc ggccattatg
     1621 cgctatcgga gcaaggatac atttaacatc gcattaaaat atccaagtat gtgagtggcg
     1681 catatcttgc cctgctgcgg cgcgccagcg cctggaaatg cgtattagat ccgcgcggcg
     1741 cttcatcggc ccttcggcta tcatctcatc aagatccgct tgcccttaca ggagaaacaa
     1801 atggcacttt cccgcgcagt gatctggatc gaccaccata acgctcaggt gctgcaattc
     1861 gatgcagagc acgtacaggc acagaagata aagtcccata cgcaccacac ccgtcaacac
     1921 ggcagcagcg tgaggacgga gcatgaattt ttcggtgatg tgtgcaacgc gctgggcggt
     1981 attgctcaag tcatgttgac gggctcgcac acggcacagt ccgattttcg tcattacgtc
     2041 gaaaagcatc gtccgtcatt ggcaaaacaa atcgtcggct gggaaacggt tgaccatccc
     2101 accgacggac aattggtggc gctcgcgcgc aaatactttg accaattcga ccgccggggt
     2161 ggcccggcca cgccggcttg agcggcttcc acgagcagcg cagcgctaga gagcgagacg
     2221 gacctctttc gacgtcgggt cgtgcggcgg ggtgtggacg gaaaagccaa gctccgcggc
     2281 gagtttgagc atctgcgtat tcaccgagag cacaagaccg atcatcgtct tcaggccccg
     2341 ggcgcgggca attgcgatca gctgcagcat gagatagcgg ccaagtccac gcccctgcca
     2401 atcctcggat accacgatcg cgtactcgca tgatgtcgca tccgggttga tgacatagcg
     2461 cgcaacgccg atctgacgtt cgcgcccctg gttctcgaca acggcaatga gtgccatctc
     2521 ccggtcgtag tcgacttgtg taaagcgtgc aagcatggcg ggcgttagat ccttcatggc
     2581 gcccagaaag cgcaggtact tcgtctgcgg cgacagcgcc tggacgaatt cacgttctat
     2641 gtgtgcgtcc tcgggacgga tagggcgcac tacaatcttg gttccatctt ttgcagacca
     2701 gtttgaagcc aattgcgccg gataaggatg gatggccaaa tgcgcgtatg caagcgcgcc
     2761 cggagcacag ggcgcgatca cgatgcgtgc gtccgccgcg cttgctccgg tggcgtcggc
     2821 cagcagcggg ttgatatcca gctcgcgtat ccatggcagt tcgcacacca tttccgatac
     2881 acgcagcagc acgttggcga cggcattgag gtcgactggc ggcatgccgc ggaatgcacc
     2941 aagcaggcgt gaaatgcggg tcgaaccgat catctcttca gcgagaaagc ggttcaatgg
     3001 cggcaaaccg actgcgcgat ctcgcaggat ttccacgatc gtgccgccgg cggcgaacgt
     3061 cactgccggg ccgaatacct tgtcggtcag caccccaacc ataagctcgc gggcgctgct
     3121 gcggttcagc atgcgttcca cggtcacccc gcgaatgcgc gcttgcggcc gggtgcgctg
     3181 cgcggaatcg ataatggcgt cgaaagccga gcgcacagct tcttcattct gcaagtcaag
     3241 gcggacacca ccgacttccg tcttgtgcgt gatgtccgga gaatctattt tcatggccac
     3301 gggatagccg cactgcattg ccgctgcgat ggcttcctgc gccgagcgag cctgcaacga
     3361 tagtgcgacc ggtatgtgaa acgcggccag caccgctttc gaatccgtgc tggagagcac
     3421 gcttgtaccg gaggcgagcg ccgtttcgat gatgcgccgc gcgccagtga tgtccggcgc
     3481 gtggtgatcg ctcaggggac ccggcacctg gataagcatg cgctgattgc ggtaaaacgc
     3541 cgaaaggtgt gcgaacagct cgacagccgg ttcgggcgtt cggaatacgg gccgctgcgc
     3601 cgtctggaac accttgcggc cttcgatcac ctgctcttcg cccatccata ccgcaagcag
     3661 cggtttcgtc gaagcggtgg cgctgcgcac gaccgcacgc gcgacctcca ccggagaagt
     3721 catggcctgc ggagtcagta tggccaatac cccatccacg ttctgatctg cgaggcatgc
     3781 agacaccgca gtttcgtagc gacccgcatc tgcatcgccg atcagatcga gcggattgcc
     3841 atgcgaccag tttaccggca gcgttttgga tagtgcttgc actgtcggcg cggaaagttg
     3901 cgcgagcgga aggcccagat cggccgcatg gtccgcggcc agcacacccg gtccaccgcc
     3961 gttcgtgaca attgcgatac gatcgccacg cggctcgaca tgtgccgaaa gcgcctgtgc
     4021 cgcggccacg agctgaccaa tcgtagccac gcgcacggca cccgtgcgcc ggaccgccgc
     4081 ctgaaacacg tcatccgcgc cgaccagcgc gcccgtatgc gaaacagccg cgcgcacgcc
     4141 ggtcggatgc cggcccgctt tcatgacgat caccggcttc gtgcgcgcgg cggcccgcaa
     4201 tgcgccgacg aagcggcgcg cattgcggat gccttcgatg taaagcagga tctgttcggt
     4261 ggcggtgtcg taggtcagat agtccacgat ctcgccgaag tccagatcgg ccgaaccgcc
     4321 cagcgatacg acgctcgaga aaccgatgtt gtttggacgc gcccagtcga gcagcgcggt
     4381 gcatatcgca cccgattgcg acacgacgcc gagcgaaccg gcgatcgcat tgccgtgggc
     4441 aaaggtggcg ttgagcccga tgcctggacg catgagccca agacaattgg ggccaagcag
     4501 gcgcatgccg tagcgtcgtg cgttttccag cagcgcgcgt tccagcatgg cgcctgcgga
     4561 tcctgtttcg gaaaaacctg ccgtgatgac aacggcagaa cgcgtgccgg ctcgtccaca
     4621 cgcatcaatg acgcctggca cactttcagg cggcgtcgcg attaccgcca gatccacttg
     4681 ttgtggaatg tctgcaatgc tgggataaca agcgactcca tgcacccgcg tccgttgcgg
     4741 attcaccgcg tacagtgcgc cagtaaattt cgcagacagc atgttttcca tgagcaccga
     4801 gcccacggcg cccggacgtt cggtggcgcc gatcaccgcg accgaatcgg gcgcgaacag
     4861 cggagcaaga tagtgagtgg atttcaaatg aatcagtagt tggtggttag tagctggtgg
     4921 ctagtggcga gtggttagta catattccta accactaata actaaccact aaccactttc
     4981 ttcagcgcca tgcgaaattc cgctgctgcc tttgcaacat tggccggcgt atcgcccggt
     5041 ttatagagcg ctgaacccag tccgaagccg tttgcaccgg cctcccaata ttcgcgcatg
     5101 ctgcccggag tgataccgcc gaccggcagt accggcacat ctttcggaag tatggcgcgc
     5161 agcgcct
//
LOCUS       DATMZQ010000029         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4844812, whole genome shotgun sequence.
ACCESSION   DATMZQ010000029 DATMZQ010000000
VERSION     DATMZQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4844812"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VN496_01000"
     CDS             complement(<1..508)
                     /locus_tag="VN496_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15545.1"
                     /translation="MAPITMANLQHLRSPMRWYIKLLTAILALVFFAVLATLAIAGFL
                     VYRIVKPQRTSSEINLQSFPGRPDAVQFMVRGGKTRDGLFFPGFRGAPTIVLCHGYGS
                     SKGELLTLVSALQDHQYNVFVFDFAAHGANDGVTSFGYREADEVRAAIDTVAQRGDVD
                     PASFGLWGY"
     gene            852..1784
                     /locus_tag="VN496_01005"
     CDS             852..1784
                     /locus_tag="VN496_01005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF15546.1"
                     /translation="MNIVDLRQISVRQVEPLLEEEARHWRDELHWDYRSALDLIKRFL
                     EAHALCGCAAFENGTAAGYSFYVLEEQKGLIGGLYVSAKYQQAVIGRRLLDEMLYSMR
                     AVPQLTRIEAQLMPFGGPVEDPLRDLGFRLYTRQFMLFDLQKSSAVRAGTSAGMRLDR
                     WNDRFFEPCAKLIYLCYTDHVDGEINDQYRSRGGALKFLKNIILLPGCGQFVPGASFV
                     LRQPGSDDLVAAVLTSEVASGVGHTTQICVLPGYQGHGLGRMLMQSSVEALRAMKFRE
                     LTLTVTSENRSAVQLYEKLGFTTIRAFTAGVWPK"
BASE COUNT          486 a          701 c          665 g          506 t
ORIGIN      
        1 tgtagcccca cagcccaaaa ctagccggat ccacatcgcc gcgctgggcc acggtatcga
       61 tggccgcgcg aacctcatca gcctcacgat acccaaacga tgtgacgcca tcgttcgcgc
      121 catgcgccgc gaaatcaaac acaaaaacgt tgtactgatg atcttgcaag gcggaaacca
      181 aggtcagcaa ctcgcctttg ctggacccat acccatggca aaggacaatc gtcggcgctc
      241 cacgaaatcc ggggaaaaac aatccatcgc gcgtcttgcc gccgcgcacc atgaactgca
      301 cggcgtccgg ccggccagga aagctttgca ggttgatctc gctgctggtg cgctgcggct
      361 tcacgatgcg gtaaaccaag aagccggcaa tcgccagggt agccaagacc gcgaaaaaca
      421 ccaaagccag aatcgccgtc agcagcttga tgtaccagcg catcggcgag cgcaaatgct
      481 gaagattcgc catcgtgatt ggtgccatag agtgtcccgc ccatcaaccg cagcacagcc
      541 aagacgctag tgtcgatggg gtaattttca tgctagcacg cgggctgcgc ggcttcgggg
      601 caactgtgtt tcgttgtgtc ccatgcgtat ctaacgattc ggcacatatc gccggcttcc
      661 tttcagggcc cggatttgtc caccttccat acgacgtggg acttgatgcc gcggaccgcg
      721 cgttgcccgc aacgatggta cgggtgatgg tgtatcagat cccgcggccc aaggaccgcg
      781 gctataggat tgccttcagt tccgttatca tggctttgtg cgacaggggt tgcacggctc
      841 tccctccatt catgaatatc gtcgatctcc ggcaaatcag cgtgcgtcaa gtcgagcctc
      901 tgctcgaaga agaggcgcgc cactggcgcg acgagctgca ctgggactat cgcagcgcgc
      961 tggacctcat caagcgcttc ctggaagcgc acgcattgtg cggttgcgcg gcctttgaaa
     1021 acggcacggc ggccgggtac tcgttctatg tcctggaaga gcaaaaaggg ctgatcggcg
     1081 gactgtatgt ctccgccaaa taccagcaag cggtgattgg ccggcggcta ctagacgaga
     1141 tgctctactc catgcgcgcc gtgccgcaac ttacacgcat cgaagcgcaa ctcatgccct
     1201 ttggcggacc ggtggaggac ccactccgcg atcttggctt ccgcctgtac acccggcagt
     1261 ttatgttgtt cgacttgcaa aaatcctcag ctgtccgcgc agggacgtct gcggggatgc
     1321 gcctggaccg ctggaacgac cgatttttcg agccctgcgc gaaactcatc tacctctgct
     1381 acacggacca cgtcgacggc gaaatcaacg accaataccg cagccggggc ggggcgctaa
     1441 agtttttgaa aaacatcatc ctgctgccgg gctgcggcca attcgtcccc ggcgcttcgt
     1501 ttgtcctgcg gcaacctggc agcgacgatc tggtcgcggc tgtactcacc agcgaggttg
     1561 cttccggtgt aggccatacg acccagatct gcgttctgcc cggctatcag ggtcacgggc
     1621 tgggccgcat gctgatgcaa agctcggtgg aagccttgcg ggccatgaag tttcgcgaac
     1681 tgacgctaac ggtcaccagc gagaatcgct cagcagtgca actctatgaa aaacttggct
     1741 ttacgacgat tcgggcgttc accgcaggcg tttggccgaa atgaagcggg gttgccccta
     1801 tgttttatag gatggttatg attctattga tgttacgcag tacttggaag catcgttgtg
     1861 actgcactgc cgttagagac gaattcgcct tccccgcgta gtacggtctg tgcttgtcaa
     1921 aggaaatcgg gattgcgatt tccgaacacg acggtagcgc tgcgtataaa tctggtccat
     1981 aaaacgattg gtttattttt ggcggcgact tgcctgcggg aacgctaatc cggagattgg
     2041 cgcttccggg ggtctgcgac gcctcaccct gacaacgcgt tgagtactgt tcattcccat
     2101 cacagcatga tcccaatttc ggacacttgc tgcactgggg ttattgaatc ggcgtccaca
     2161 agcggtttgc agtcaacgta ttaccacggc cggagctgga ctcccgcgtg cttttgccag
     2221 tccatccctc gaccgctcct cgctggaggc tcagcccgtg tcaaccatca tccaggattt
     2281 gcgctttgcc atccgcatac tgatcaagaa tccgggcttc tctttgatcg cgatcgtgtc
     2341 gctggcgctg ggcatcgg
//
LOCUS       DATMZQ010000030        14438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3821656, whole genome shotgun sequence.
ACCESSION   DATMZQ010000030 DATMZQ010000000
VERSION     DATMZQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3821656"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VN496_01010"
     CDS             complement(<1..883)
                     /locus_tag="VN496_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXF15547.1"
                     /translation="MAKFGIGQAMLRREDQRLLFGTGQYVDDVALANEVFVAFVRSPH
                     AHARILSIDAASAKTMPGVIAVLTGADLKADDIGPLPDGQGLKRADGKDMCGPPHDVL
                     VVDTARYVGEPVAAVFAQSRDQAEDAVEQVMVEYEELPAVVTIAAATAKGAPQLWPDA
                     PGNIAAQTEFGKKDACDAAFAKAKHITRLSLHNQRLIPVSMEPRGSIAQFDAGTGRIT
                     LRTSCQNPAGLQKTLAESCLKVPAESVRVVVGDVGGGFGMKTQLYAEDVVCAWGARKL
                     KRPVHWRASRSEEFLGGN"
     gene            1061..1525
                     /locus_tag="VN496_01015"
     CDS             1061..1525
                     /locus_tag="VN496_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXF15548.1"
                     /translation="MSVRLSMTVNGKPVNADVEPRTLLVDFIRDTLRLTGTHIGCDTA
                     QCGACTIHMNGKAVKCCNMLAVQAQGSTVVTIEGLAKADGTMHPMQAAFRDHHGLQCG
                     YCTPGMVMSAIDLLKRHPHPNEQQIRAELDGNLCRCTGYHNIVRAVQAAAEA"
     gene            1557..2357
                     /locus_tag="VN496_01020"
     CDS             1557..2357
                     /locus_tag="VN496_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXF15549.1"
                     /translation="MHAFNYHKASSVADATAKLAASDDGRLLAGGQTLIAAMKMRLSA
                     PSDLVDLAGIAELRGIKVDASSATVGAMTTHATVAASPEIKKAIPALALLASEIGDPM
                     VRNMGTMGGSVANNDPAADYPAAVLGLGATVLTSKRKIPADKFFTAMYETALEPGEII
                     TSIAFPVPKRAGYSKFKNPASRYALVGVFVADTANGIRVAVTGAGPCVFRLPELEKAL
                     GKSFSPDAVNGIKVPATNLNADIHASAEYRAHLITVMAKRAVSQILSK"
     gene            2505..3419
                     /locus_tag="VN496_01025"
     CDS             2505..3419
                     /locus_tag="VN496_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXF15550.1"
                     /translation="MMTRPAVTIPTSIDDTLALLARGEYVAERSLATAVFLALRMGRP
                     LFLEGEAGVGKTEIAKVLAQVLGRQLIRLQCYEGLDIASAVYEWNYQRQMIEIRLAEA
                     EGADGRERIEKDIFSERFLIRRPLLQALDSRAGEAPVLLIDELDRTDEPFEAYLLEVL
                     ADFQVTIPEIGTIRAQCPPIVVITSNRTREIHDAVKRRCLYHWVDYPTAVAELEIVRR
                     KVPGLAEKFSTDIVAFVQKLRTADLFKRPGIAETIDWAKALVELDRRSLDATVVNDSL
                     GVLLKYQDDIASVRGSEAARILAEIKSH"
     gene            3421..4641
                     /locus_tag="VN496_01030"
     CDS             3421..4641
                     /locus_tag="VN496_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXF15551.1"
                     /translation="MQLSQNVVHFARALRKAGVPVGPDRVMDGLRALELSGIERREDF
                     YWTLAAVFLSRREQFELFDQAFWLFWRERHLLDHALQDLPVAAPSNPSASQISNRIAE
                     ALGLTKKLPMRLNSEEEEPDVLLSPSDLERLQRRDFETMTSEELEQAKRLIAGLRLPI
                     PEIRTRRLHPDACGHRVDLRATLRASLRGNADIIALRRRSYRLRHPPLVVLCDISGSM
                     SRYSRMFLHFLHAITSDRDRVHSFVFGTRLTNISRQLRHRDVDVALAAISDSVEDWSG
                     GTRIGATLKEFNQRWSRRVLAQNATVMLISDGLDRDAGEDLTVQMERLHKSCHELIWL
                     NPLLRFEDFQPKAAGVRLMLPHVDAFLPAHNIDSLTDLARSLRPKPVFGNELRRFGRP
                     FAVLNKPGDSATWK"
     gene            4632..5198
                     /locus_tag="VN496_01035"
     CDS             4632..5198
                     /locus_tag="VN496_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HXF15552.1"
                     /translation="MEMSGEQRIPLSQQQVWEALNDPEILKACIPGCESIERVSDNEY
                     KVAMTAAVGPVKAKFSGKLQLADLDPPNAYSLAFEGSGGAAGFGKGSAQVRLVPEGND
                     ATLLTYKASASVGGKLAQIGSRLIDGVARKMADDFFERFNNVVAPAGQPASHMPGSVP
                     PSHRAPIPGWLWAIGGILALLILLYLFR"
     gene            5336..6085
                     /locus_tag="VN496_01040"
     CDS             5336..6085
                     /locus_tag="VN496_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF15553.1"
                     /translation="MNQLDMKGRSAVITGGASGIGLAIAKRLAQSGANVALWDMNEKA
                     MSDAARSLGAVKTHTAKVDVSKLDQVEAAMKSTLAAFGKIDALVNSAGIAGKNATVWE
                     TPVDDWIRVHDINLNGTFYTCRTVVPHMMKNNYGRIVNIASIAGKEGNPNASAYSSSK
                     AAVIGLTKSLGKELAKNNITVNCVTPAAVKTPIFDQVAQSHIDYMLAKIPMGRFGLVE
                     EIAATVSWACTEDCSFTTGAVFDLSGGRATY"
     gene            6172..7296
                     /locus_tag="VN496_01045"
     CDS             6172..7296
                     /locus_tag="VN496_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXF15554.1"
                     /translation="MKIRAAVIRQMGLPQPYADSKPMSIEQVELDKPGQREVLVQIKA
                     ASLCHSDLSTVNGDRPRQMPMVLGHEASGVVQEVGGGISDLSVGDHVALVFAPSCGQC
                     LPCVEGHPGRCEPGQQANGAGRLLNGGMRLHQHGKDVYHHVGVSAFAEYCVVNRGSLV
                     KIDKALAFDEAALFGCAVLTGVGAVFNTARVFPGAKVAVLGLGGVGLNSLLGAIVAGA
                     QQIIALDLHDDKLKLAKDLGATDTVNAKDPDAIQKVKDLTGGGVHFALEMAGSVQAME
                     LAYRITRRGGTTVSAGLPHPDHRWPLQHVNMVAEERTIKGSYVGSCVPARDIPRYIDL
                     YRRGMLPVNKLMSDHIKLEEINVGFDRLASGHTVRQIIMM"
     gene            7302..8453
                     /locus_tag="VN496_01050"
     CDS             7302..8453
                     /locus_tag="VN496_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HXF15555.1"
                     /translation="MARSAIARDGHNGVTAWLNPPPTNRSEGHAMTATRYRAEDLIRY
                     ATALLEQAGLESEKCKIVAEILLEGDLLGHTTHGLQLLGPYLNDLEKGGMTKSGMPRV
                     IADFPAAVTWDGMRLPGTWLTAHAIDIALERATKYGTCTVAIRRSHHIACLAAYLKRV
                     TDQGMMVMLISSGPETGGVVPHGGRGTGTHTPNPIAAAWPTKGDPVMIDVSMSITTHG
                     LTGRLHREKRKLPGQWVLDGHGNPSSDPAVLFNEPKGGLLPIGGLDHGHKGYALGLLV
                     EALTQGLAGHGRADAYEGWSANVYVQVFDPALYAGCEDFTHQTEWIAEACRTTPPRAG
                     FDRVRLPGESGLRRREEQMQHGVELYPGIMTGLEPWSDKLRVPAPAAIV"
     gene            8453..9325
                     /locus_tag="VN496_01055"
     CDS             8453..9325
                     /locus_tag="VN496_01055"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF15556.1"
                     /translation="MRTPTEHRRGIALMVGATLCWATAGILVRNMDATDGWKITFWRS
                     LFMTVFMIAVLGFQHGGRLPRRIRAMGWPGVLSGFLFAWMMICFILALSLTTVANTLV
                     VGSISPFVAALCGYLFLGEKVPPRTWMAMLAALGGIVTMFFDALSDGGWAGNLIALCI
                     PVGFGANVVLLRKHRASFDMIPAVLLAGVFSMLIALPFAWPLTVSIADLALLSVMGVL
                     QLGIGLLLMLSAVRYLVSAEIGLLSILEIVFGTLSVWLVIGERPSQTALIGGSMVLAA
                     LMINEIAGLRPATA"
     gene            complement(9455..10522)
                     /locus_tag="VN496_01060"
     CDS             complement(9455..10522)
                     /locus_tag="VN496_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXF15557.1"
                     /translation="MKVSIIAVAAGLAFALSAAVVQAQVSDKPFQENWAPTKWGKDDS
                     AGSSNHTKNPANIARAISMIKQNKSVTLGKYYHKDIPAVGERGWKMVLPGTPHAGPFG
                     KNAMIYHDEYVSTEIGQIGTQFDGPGHIGVNTTKGMFMYNGRMYPASYERGGGGRAVG
                     MGPNGVENVGENGFICRGIVLDAAGYRGVERLPIPKKPGEIGIVTADDVKAMIKRQGI
                     IEPQPGDCVFLHTGHGNIWGNSVYKKMNSAQHAKAREEFGAGEPGFGISACEYMASRD
                     IALMGGDTSSNDAQPSGEFGEDYAVHCHTEMQTRRGIWNLENLDLKPLVDAKVYEFAF
                     IWAPLKIIGGTGSPGNPIAIY"
     gene            complement(10879..11244)
                     /locus_tag="VN496_01065"
     CDS             complement(10879..11244)
                     /locus_tag="VN496_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15558.1"
                     /translation="MSAAIAACDAFTDAATRIAYDIEAGASRLGPENGARYSIEHRTP
                     SKEGQCIGPYTVQLDKVGALIVWCKDETGKTVSSHSTSYHARFVDTPWTYLIDKPARS
                     TLVIDIERRGNRPVIINVS"
     gene            complement(11304..11837)
                     /locus_tag="VN496_01070"
     CDS             complement(11304..11837)
                     /locus_tag="VN496_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15559.1"
                     /translation="MIAKPAKSIPSRQQLLVGFVAAISGTFIVSIALGLIPAPEESFH
                     APHWVVGLAGLVFVLAGASFLAPSLVAGLRAVDQYTPAQQKAIDLVRQILGCLLLTAF
                     AAVPLWIGFGAGERMFSLSFGFSGLASEGPGRETTGRMIFGAAGILVAVWALFAWVAL
                     VRSLIATLDRTNDQAAK"
     gene            complement(11890..12135)
                     /locus_tag="VN496_01075"
     CDS             complement(11890..12135)
                     /locus_tag="VN496_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RseA family anti-sigma factor"
                     /protein_id="HXF15560.1"
                     /translation="MSLKVSLLLDGQLASAEVKAALDETAADERLRDRYTLYRLVGDV
                     LRGNSTPDDGYSERILQRMRREGSRIESGYDPLADRE"
     gene            complement(12138..12731)
                     /locus_tag="VN496_01080"
     CDS             complement(12138..12731)
                     /locus_tag="VN496_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXF15561.1"
                     /translation="MVIADQDAELVRLAQTGDAKAFEALVVKYQRRIARHVARYLRHS
                     ADVEDVVQEVFIRAHRGLASFRGDSAFFSWLYRIASNAALSHLKRSPEAVQLGDDAPD
                     ERAEAFEPGISDAADPERTLIAKQIADTVQRALTKLQPDLAEALMLYEVEGKSYAEIA
                     GMLNVPIGTVRIRIYRAREFIAKRLESVLGPQRNRRW"
     gene            13034..13498
                     /locus_tag="VN496_01085"
     CDS             13034..13498
                     /locus_tag="VN496_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HXF15562.1"
                     /translation="MKSLSRYIAGAVLAAASSFAFAADIVDTAVSAGQFKTLVKAVQE
                     AGLVDTLKSKGPFTVFAPTDEAFAKLPAGTLDALLKDKQKLAAVLTYHVVPGRVMAAQ
                     VKPGQVKTVEGQSLTVTTMNGAVMVNNAKVLKTDIVASNGVIHVIDTVVLPQ"
     gene            complement(13580..>14438)
                     /locus_tag="VN496_01090"
     CDS             complement(13580..>14438)
                     /locus_tag="VN496_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXF15563.1"
                     /translation="QHFDVFKDAGVVLNEFGWILTGDDGKVRYYAAYPAEKVPFWLKL
                     TATGTPGHGSAPRPDSAVLKLVEALHRVATYKTPLKVEDIVQKFYAQTAHLDPVAERR
                     ERLKDLRKALLDPVFATEFTKDLRANAMVRNTISITMLEGSNKVNVIPPEASAQLDVR
                     LLPSEDPQRFLRALKNVIADDSIRIEPVLSFPPSTSPVDSEFFDVLREVANVYDPGTL
                     VTAPMITGFTDCHYFREKGIPCYGFMPFKLMPKDLGGVHGNDERVSIDNVIFGTEVMT
                     EIVRRLATQ"
BASE COUNT         2952 a         4320 c         4157 g         3009 t
ORIGIN      
        1 gattgccacc cagaaattcc tcgctgcggc tagcacgcca atgcacggga cgcttcaact
       61 tgcgcgcccc ccacgcgcac acgacatctt cggcatacaa ctgcgtcttc atgccgaaac
      121 cgccgccgac gtcaccgact acgacgcgca cgctttcggc cggcaccttg aggcacgatt
      181 ccgccaaggt tttctgcaag cccgccggat tctggcaact cgtacgcaac gtgatcctgc
      241 cggtgcccgc gtcaaattga gcaatactgc cgcgcggttc catgctcacc gggatcagtc
      301 tctggttgtg cagcgagagc ctggttatat gcttggcttt ggcgaaagcc gcgtcgcatg
      361 catctttctt gccgaattct gtctgcgcgg caatgttgcc cggcgcatcg ggccacaact
      421 gtggcgcacc tttagcggta gcagctgcta tggtcacaac ggcgggcaat tcctcgtatt
      481 ccaccatcac ctgctcgacc gcatcttccg cctggtcccg gctttgcgcg aatactgcgg
      541 cgaccggttc gccgacatag cgcgccgtat cgacaaccag cacgtcgtgt ggcgggccgc
      601 acatgtcttt gccatctgcg cgcttgagcc cctggccatc cggcaacggg ccgatgtcat
      661 ccgccttgag gtccgcaccg gtcaataccg caatcacacc cggcatcgtt ttggcggatg
      721 ctgcatcgat agaaagaatg cgcgcgtgcg catggggcga gcgcacgaat gccacgaaaa
      781 cctcgttcgc cagtgcaacg tcgtccacat attgccccgt gccgaacaga aggcgctgat
      841 cttccctgcg cagcatcgcc tgaccaatac cgaactttgc catgtcgtta tcccgtttcc
      901 gttatgctgt aaaaagaact gtcgccggat tttacgccac cccgacccgg atgtttaacg
      961 ccggcagcgt tccgttcagg ttgccccggc tgacggggtt gtctaggatt gcacttttcg
     1021 ccgcgcatct tcggcttttc cccaccacaa ggattaattc atgtcagtcc ggctatcaat
     1081 gaccgtaaat ggaaaacccg tcaacgctga cgtggaacca cgtaccctgc tggtggattt
     1141 catccgcgac acactgcgcc tgaccggaac ccatattggg tgcgataccg cacaatgcgg
     1201 cgcctgtacc atccatatga atgggaaggc agtcaaatgc tgcaacatgc tggccgtgca
     1261 ggcacagggc agcaccgtcg taaccattga aggcttggcc aaggcagacg gcaccatgca
     1321 tcccatgcag gcggcatttc gggaccacca cgggttgcag tgcggttatt gcaccccggg
     1381 catggtcatg agcgccatcg atctgctcaa gcggcatccg catccgaacg aacagcagat
     1441 ccgcgctgaa ctggacggca acctgtgccg ctgcaccggc tatcacaaca ttgtcagggc
     1501 cgtgcaggcc gctgccgaag catgagtatc aatgccgcca tctaaaggac atcagcatgc
     1561 acgcattcaa ttaccacaag gcctccagcg tcgcggacgc aacggcaaaa ctagcggctt
     1621 cggatgacgg caggctgctg gcgggcggcc agaccctgat tgcagcgatg aaaatgcgtc
     1681 tgtccgcgcc gtctgatctg gtcgacctgg cagggatcgc agagttgcgc ggtataaagg
     1741 ttgacgccag cagcgccacg gtcggggcaa tgacgacgca cgccacggtg gctgcttcgc
     1801 ccgaaataaa aaaagccatt ccggcgctgg cgttacttgc ctcggaaata ggtgatccca
     1861 tggtccgcaa catgggcacc atgggcggtt cggttgccaa caacgatcct gcagcagatt
     1921 acccggccgc ggtgttggga ctcggggcaa cggtgctgac cagcaaacgc aaaatccctg
     1981 ccgataaatt ctttacggca atgtatgaaa ctgcactcga gcccggcgaa atcattacct
     2041 cgatcgcttt tccggtaccg aaacgtgccg ggtacagcaa attcaaaaat ccagcctcgc
     2101 gctatgcgct ggtcggcgtc ttcgtcgccg atacggctaa cggtatacgc gttgccgtga
     2161 ccggcgcggg accgtgcgta ttccgccttc cagaactgga aaaagcgctt ggcaaatctt
     2221 tttcgcccga tgcggtcaat ggcatcaaag tgccggccac aaatctgaat gctgacattc
     2281 atgccagcgc tgaataccgt gcccacctga ttaccgtgat ggcgaagcgc gctgtctcgc
     2341 aaatcttgtc gaaatagtgc ccgtgtttaa acatcggcag cgatcccgga acacgcaaat
     2401 atagaacctg cagcacgtgg tcgggaaagc gattcgccgt attgctttgg agtcagtgcg
     2461 ccgctgtctc ggcaacgctg tattgagaca tttagttctg gaatatgatg actcgtcccg
     2521 ccgtaactat tccgacttcc attgatgaca cgctggctct gctggcgagg ggcgaatatg
     2581 tcgccgagcg ctcactggcc actgccgtat ttctggcatt gcgtatggga cggcccttgt
     2641 ttctcgaagg cgaagcagga gtgggcaaga cggaaatcgc caaggtgctg gcgcaggtcc
     2701 tcggccggca gctcatccgg ttgcaatgtt acgaagggct ggacatcgca tcggctgtat
     2761 acgaatggaa ttaccagcgt cagatgattg aaattcgcct ggcggaagcg gaaggcgcgg
     2821 atgggcgcga aagaatcgag aaagacatct tcagcgaacg ctttctaatt cgccggccgc
     2881 tgcttcaggc gctggattca agggccggcg aagctcccgt gctgctgatt gacgaactgg
     2941 atcgcaccga cgaacccttc gaggcttacc tgctggaagt tctagcggat ttccaggtca
     3001 ctattccaga gatcggcacg atcagggcgc agtgtccgcc gattgtggtc atcacctcga
     3061 accgcacgcg tgaaattcac gacgcagtca agcgccggtg cctctatcac tgggtggatt
     3121 atccgactgc cgtggcggaa ctggaaatcg tccggcgcaa agtgccagga ctcgccgaaa
     3181 agttttccac cgatatcgtc gccttcgtgc agaaattgcg aacggccgat ctgttcaaac
     3241 gtcccggcat cgccgaaacc atcgactggg cgaaggcgct ggtcgaactt gatcgccgat
     3301 ccctcgacgc cacggtcgtc aatgacagcc tcggcgtgct gctcaagtac caggacgaca
     3361 ttgcatccgt gcgcggcagc gaagcagccc gcatcctcgc tgaaatcaag agccactagc
     3421 atgcagctgt cgcaaaatgt tgtgcatttc gcccgggcgc tgcgtaaagc cggcgtgcca
     3481 gtggggcccg atcgcgtcat ggatggcttg cgcgcgctgg agctatcggg catcgaaagg
     3541 cgcgaagatt tctactggac gctggctgcg gtttttcttt cccggcgtga gcaattcgaa
     3601 ttgttcgacc aggccttctg gctgttctgg cgcgaacgcc atctgcttga ccatgcattg
     3661 caggacttgc cggttgccgc gccgtcgaac ccctcggcca gccagatatc gaaccggatc
     3721 gccgaagcgc tggggctgac gaaaaaattg ccgatgcggc tcaactcgga agaagaagag
     3781 cccgatgtcc tgctgagccc atccgatctc gagcgcctgc aacgccgcga tttcgagacc
     3841 atgacgtcag aagagctgga gcaggcgaaa cggctgattg ccgggctgcg cctgccgatt
     3901 ccggagattc gcacacgccg gctgcatccc gatgcatgtg ggcaccgcgt ggacttgcgc
     3961 gcgaccttgc gcgcgagtct ccgcggcaac gccgatatca ttgcgctgcg ccgccgctcg
     4021 taccgactgc ggcatccgcc cctggtcgtg ctttgcgata tctccggttc catgagccgt
     4081 tattcgcgca tgttcctgca ttttctgcat gccatcacca gcgatcgcga ccgcgtgcac
     4141 agtttcgtgt tcggtacgcg tcttaccaac atatcgcgtc aacttcgaca tcgcgacgtg
     4201 gatgtggcac tggcagcaat atccgactcg gtggaggact ggtccggtgg cacccgcatt
     4261 ggcgccacgc tgaaagaatt caatcagcgc tggtctcgcc gcgtgctggc ccagaatgcc
     4321 acggtcatgc tgatcagcga cggcctcgat cgggatgccg gcgaggacct gaccgtccag
     4381 atggagcgcc tgcacaaatc ctgtcacgag ttgatctggc tgaatccgtt gctgcgcttc
     4441 gaggacttcc agccgaaggc ggccggcgtg cggctgatgc tgccgcacgt ggatgctttc
     4501 ctgcctgcgc ataatattga cagcctgact gacctggcgc gctcgctgcg acccaaacct
     4561 gtgtttggaa acgagctccg cagattcggg cgtccatttg cagttctgaa caagcctgga
     4621 gactcggcca catggaaatg agcggcgagc aacgcatccc tctttcgcaa caacaagtgt
     4681 gggaagcgct caacgatccg gagattctaa aggcctgcat ccctggctgc gaaagtatcg
     4741 agcgggtatc ggacaacgaa tacaaagtag cgatgaccgc tgcagtcggt ccggtgaaag
     4801 caaagttctc cggcaagttg caacttgccg atctggatcc gccgaatgcc tactcgctcg
     4861 cattcgaagg ctccggcggt gctgccggat tcggcaaggg cagcgcgcaa gtcaggctcg
     4921 tgccggaagg caacgacgcc accttgctga cctataaggc gagcgcgagc gtaggcggca
     4981 aacttgcaca aatcggctct cgactgatcg acggggtggc tcgaaaaatg gcggacgact
     5041 ttttcgaacg cttcaacaat gttgtcgcgc cggccggcca accggcgagc cacatgccgg
     5101 gctccgtccc gccatcgcat cgtgcaccca ttcctggctg gctctgggcc atcggcggca
     5161 tcctcgccct tttgatcctg ctctacctgt tcaggtagtc ggcatttgac gatggcatcg
     5221 actgtcgcag gagatgccat tgatcgaagg aaaaaggggc ctgctaaaat gccggccttc
     5281 caaagcccgt ttgtccttgg catgccgggc agatcaccaa taaacaggga gcgttatgaa
     5341 tcaactcgac atgaaaggcc gcagtgccgt gattaccggc ggtgcatccg gcatcggcct
     5401 cgcgatcgcg aagcggcttg cgcagtcggg cgccaatgtt gcgctctggg acatgaacga
     5461 aaaagccatg agcgatgcag cgagatcgtt gggcgcggtg aaaacccaca ctgccaaagt
     5521 cgacgtgagc aagcttgatc aagtcgaagc tgcaatgaag tccactttgg ccgccttcgg
     5581 caaaattgat gcgttagtca actccgccgg gatcgccggc aagaacgcca cggtatggga
     5641 aaccccggtg gacgactgga tccgcgtgca tgacattaat ttgaacggca ccttttacac
     5701 ctgccgcaca gtcgtgccgc acatgatgaa gaacaattac ggccgcattg tcaacattgc
     5761 ctccatcgct ggcaaggaag gcaatccaaa cgcttcggcc tacagctcct cgaaggcagc
     5821 cgtaatcgga ctgaccaaat cactgggcaa agaactcgca aagaacaata ttacggtgaa
     5881 ctgcgtcacg ccggccgccg tgaagacgcc catctttgac caggtggcgc agagccatat
     5941 cgactacatg ctcgccaaaa tcccgatggg tcgttttggc ctcgttgaag agatagccgc
     6001 aactgtctcg tgggcctgta ccgaggattg ctcgttcacc accggcgcgg tgttcgatct
     6061 ttccggcgga cgcgctacct attagtcaac ttcgatcaaa tcattgaact accgggaaat
     6121 cacatcggtt tatctgtgct tctccatggt caaatctctc aaggtgcagg aatgaaaatt
     6181 agggcagcag tcatccggca aatgggctta ccgcagcctt acgcggacag caagccgatg
     6241 agcatcgagc aggtcgaact ggacaagccg ggccagcgcg aagtgctggt gcagatcaag
     6301 gcggcaagcc tttgccattc ggatctttcg actgtcaatg gcgaccgtcc tcggcaaatg
     6361 ccgatggtgc tgggccacga ggcttccggc gtcgtgcagg aggtaggcgg cggtatcagc
     6421 gacctgtcgg tcggcgatca cgtggccctg gtgtttgcac ccagttgtgg ccagtgcttg
     6481 ccctgcgtgg aaggtcatcc cgggcgctgc gaacccggtc agcaagccaa tggcgcgggt
     6541 cgattgctca atggcggcat gcggctgcat cagcacggca aggatgtcta tcatcacgtg
     6601 ggcgtgtcgg cgtttgccga gtattgcgtg gtgaaccgtg gttcgctggt gaagattgac
     6661 aaggcgctgg cattcgatga agccgccttg ttcggttgcg cggtgctcac cggagtaggt
     6721 gcggtcttca ataccgcgcg ggtctttccc ggcgcgaaag tcgcggtgct gggactgggt
     6781 ggcgttggat tgaattcact gctcggcgca atcgttgccg gcgcacagca aatcatcgcc
     6841 ctcgacttgc atgatgacaa actcaaactg gcaaaagatc ttggcgctac ggataccgtc
     6901 aacgcaaagg acccggatgc aatccagaag gtcaaggacc tgacgggcgg cggagtgcac
     6961 ttcgcgctcg aaatggccgg ctccgtacag gcgatggaat tggcgtatcg catcacccgg
     7021 cgtggcggca ccacagtgtc tgccgggctg ccgcatcccg atcatcgctg gcctttgcag
     7081 catgtcaaca tggtcgccga agaacgcacc atcaaaggca gctacgtcgg gtcttgcgtc
     7141 cctgcacggg acattccccg ctacatcgat ctttatcgcc gcggcatgct gccggtgaac
     7201 aagctcatga gcgaccatat caagcttgaa gagattaatg tcggctttga tcgcctggct
     7261 tccggccata cggtccgcca gatcatcatg atgtgatggc agtggcgcga agcgccattg
     7321 cccgtgacgg tcataacggc gtcaccgcgt ggctcaatcc gccacccacc aatcgcagcg
     7381 aaggccatgc aatgactgca actcgttacc gcgcagaaga cctcatccgg tatgcaaccg
     7441 cactgctcga gcaggcaggt ctggaatcag aaaaatgcaa aatcgtcgct gaaattctgc
     7501 tcgaaggaga tctgctcggc cataccacgc acggcttgca attgctggga ccttatctga
     7561 acgatctgga aaagggtggc atgaccaaat ccggaatgcc gcgcgtgatc gcagatttcc
     7621 cggctgcagt gacgtgggac ggcatgcgtc tgccgggaac ctggctcact gcgcatgcca
     7681 tcgatatcgc gctcgagcgc gccacgaaat acggcacgtg cacggtagct attcgccgca
     7741 gccaccacat cgcctgcctc gctgcatatc tgaaacgcgt caccgaccag ggcatgatgg
     7801 tgatgctgat ttcttccgga ccggagactg gcggcgtggt gccgcacggc ggtcgcggta
     7861 ccggcacgca tacgccgaat cccatcgctg cagcgtggcc gacgaagggc gatccagtca
     7921 tgatcgatgt gtcgatgtcg ataaccacgc atggtttgac cggcaggctg caccgggaaa
     7981 aacgcaagct tccgggacaa tgggtgctcg acggccatgg caatccttcg tccgatcctg
     8041 cggtgttgtt caatgaaccg aaaggcgggc tgctcccgat tggtgggctc gatcacggcc
     8101 acaagggtta tgcgctcggg cttctggtgg aagcgttgac gcagggcctg gcaggccacg
     8161 gacgcgcgga tgcatacgaa ggctggtcgg cgaacgttta cgtgcaggtg ttcgatcccg
     8221 cgctgtacgc gggctgcgag gactttacgc accagaccga atggatcgcc gaggcttgcc
     8281 gtactacgcc gccgcgcgcc ggctttgatc gcgtgcgcct gcccggagaa tcgggcttgc
     8341 gccgccgcga agaacaaatg cagcatggcg tcgaactgta tcccggcatc atgaccgggc
     8401 tggaaccctg gagcgacaaa ctgcgcgtgc ctgcacctgc agcaatcgtc tgatgcgcac
     8461 gccaacagaa caccgcaggg gcattgcgct catggtgggc gccacgttgt gctgggcgac
     8521 ggccgggatt ttggtgcgca acatggacgc gaccgacggc tggaaaataa ccttctggcg
     8581 atccttgttc atgacggtct tcatgattgc cgtattgggg ttccagcatg gcggccggct
     8641 gccgcggcgc attcgcgcca tgggctggcc cggcgtgctg tccggcttct tgtttgcatg
     8701 gatgatgatc tgtttcatcc tggccctatc gctgacgacc gtcgcgaaca cgctcgtggt
     8761 aggcagcatt tcgccgttcg ttgcagcgct ttgtggctat cttttcctgg gcgaaaaagt
     8821 cccaccccgc acctggatgg ccatgctggc tgcgctgggc ggcattgtca ccatgttttt
     8881 cgatgcgcta tcggatggcg gctgggccgg aaatctgatc gccctctgca ttccggtcgg
     8941 gttcggggcg aatgtggtgc tgctcaggaa gcaccgcgcc tcgttcgaca tgattcctgc
     9001 agtgttactc gcgggtgtct tttccatgct gatcgcactg cccttcgcct ggccgctgac
     9061 ggtcagcatc gccgacctcg cgctgctttc tgtcatggga gtactgcagc tcggtatcgg
     9121 gctgttgctg atgctctccg cagtgcgcta tctcgtttcc gcggagatcg ggttgctttc
     9181 cattctggaa atcgtattcg gcacgctgtc ggtgtggctg gtgattggcg agcggccaag
     9241 ccaaaccgcg ctgatcggtg gcagcatggt gctggcagca ctcatgatca acgagatcgc
     9301 cggcctgcgg cccgcaacgg cataagagca gcatccttga cgaaaggctg gagttcaatc
     9361 gattggttga accccatata gaagaacgtg accaaaaaaa ggcggcaact tatgtcgccg
     9421 cctgcagtga ggtctttcgc cgcgtagtgc ctgatcaata aatggcgatg ggattgccgg
     9481 gcgatcccgt gcccccaata atcttgagcg gcgcccagat aaacgcgaac tcgtaaacct
     9541 tggcgtcgac caatggtttc agatccaggt tttccaggtt ccagatgccg cgccgggtct
     9601 gcatctccgt gtggcaatgc acggcatagt cttcaccgaa ctctccggaa ggctgtgcat
     9661 cattcgacga cgtgtcgcca cccatgagcg cgatatcgcg ggaagccatg tattcgcagg
     9721 cgctgatgcc gaagccgggt tcaccggcac cgaactcttc gcgcgccttt gcatgctggg
     9781 cggaattcat cttcttatac acgctgtttc cccaaatgtt gccgtgcccc gtatggagaa
     9841 acacgcaatc accgggctgc ggctcgataa tgccctgccg cttgatcatt gccttcacat
     9901 cgtctgccgt aacgatgccg atctcgccgg gttttttcgg aatgggcagc cgctcgacgc
     9961 cgcgataccc tgccgcatcc agtacgatgc ctcggcaaat gaatccgttc tctccaacat
    10021 tttcgacacc gttcgggccc atgccaactg cgcgcccgcc acctccgcgt tcataagatg
    10081 ccgggtacat gcgaccgttg tacatgaaca tgcctttcgt agtgttcacg ccgatatggc
    10141 caggaccgtc gaactgcgta ccgatctggc caatttccgt cgagacgtac tcatcgtgat
    10201 aaatcatggc gttcttgccg aaaggtccgg cgtgcggtgt gcccggcagc accattttcc
    10261 agccgcgctc gccaactgcc gggatatcct tgtgatagta cttgcccagc gtcaccgact
    10321 tgttctgctt gatcatgctg atggcccgcg cgatgttggc gggattcttg gtatggtttg
    10381 acgagcctgc tgagtcgtcc ttgccccatt ttgtcggagc ccagttctcc tggaacggtt
    10441 tgtcgctgac ctgcgcctgg acgacagccg ccgataacgc aaaggccaag ccggccgcta
    10501 cggcgatgat tgaaactttc ataatatctc cttcccctga ttgtttttgg atgactgcga
    10561 tattgcgggc cgggggacag catcaacaac accggcttga aaatgtccac tccccatacc
    10621 ggtcgcactc aacgcccagc cgcgccggga cgccggaaca ctttactccg ctttttgttc
    10681 ggggcggaaa tggtcgaaac gcatactggg gatggcagcc ctgctgcccc gtgttgcacg
    10741 ccaggaatca gttccacctg cagtgtgatt gatgcagcgc gctgattggt gcgcgtggtg
    10801 ccagcgacaa catccttctt ccagcggctc gccgctggtg tctcgatgcg gcgactggtg
    10861 gtcgcgtgaa gccacaattc aggatacgtt gatgataacc ggccggttac cgcgcctctc
    10921 aatatcgata accagggtcg aacgtgccgg tttatcgatc agatatgtcc acggcgtatc
    10981 gacaaaccgt gcatggtagg acgtgctgtg gctggatacc gtcttcccgg tttcgtcctt
    11041 gcaccatacg atcagggcgc ccaccttgtc gagttgaacg gtgtagggac cgatacactg
    11101 gccctccttt gagggcgtcc tgtgctcgat gctgtatcgc gcgccgttct cagggcccag
    11161 tcgcgaggcg ccggcttcaa tgtcgtaagc gatacgcgtg gcggcatcgg taaaagcatc
    11221 gcaagccgca atcgcggccg acatcattat tagccagcac cacctgcggc aaattttttc
    11281 tgaagactgg ctgttgatca gagtcacttc gccgcctggt cgttcgttct gtccagcgtc
    11341 gcgatcaggc tccgcaccag ggcgacccac gcaaacaacg cccatactgc gaccaatatc
    11401 cctgccgcgc cgaagatcat gcgtcccgta gtctcacggc ctggaccttc gctcgccaaa
    11461 ccggagaaac cgaacgataa gctgaacatg cgttcaccgg cgccgaatcc gatccacagc
    11521 ggcacggccg caaaagcagt caaaagcagg catcccaaga tctgtcggac aagatcgatc
    11581 gctttctgct gggctggcgt gtactgatca accgcacgca accctgccac cagcgacggc
    11641 gcgagaaatg aagcgcccgc caatacgaaa accaatccgg caaggccaac tacccaatgt
    11701 ggcgcgtgga atgattcttc cggtgcggga atcagaccca acgcaatcga gacgatgaaa
    11761 gtgcccgaaa tagctgccac gaatccgacc aacagctgtt gtctggacgg tatggacttt
    11821 gcgggcttcg ctatcacttt gttgatccgc gttgcgttat tgcagctgca tcaaaactgg
    11881 actcgcccgt cattcccgat cggccaatgg atcatatcct gactcgatgc gggaaccctc
    11941 gcgccgcatg cgttgaagaa tacgctcgct gtagccgtcg tccggagtgc tgttgccacg
    12001 cagaacgtct cccaccagac gatagagtgt gtagcggtcg cgcagccgct cgtcggcggc
    12061 agtttcatcc agcgccgcct tcacctcagc ggaagcaagt tgtccatcca gcaacagaga
    12121 gactttcagt gacatgctca ccacctccta ttgcgttgcg gcccgagcac cgattccagg
    12181 cgcttggcga taaattcgcg ggcacgataa atccggatgc gtacagttcc gattggaaca
    12241 ttcagcatcc cggcaatctc cgcgtaggat ttgccttcga cttcatagag catcagggcc
    12301 tcggccagat caggctgtag tttcgtgagc gccctttgca cggtatccgc gatctgtttg
    12361 gcaatcaacg tgcgctcggg atcggctgca tccgaaattc cgggctcgaa cgcctccgct
    12421 cgctcatccg gcgcgtcgtc acccaactgc acagcttcgg gcgagcgctt caggtgactt
    12481 agcgcggcgt tacttgcgat gcgatagagc caactgaaga atgcgctgtc ccctcgaaac
    12541 gaggcgaggc cgcggtgagc gcgaatgaat acctcctgga caacgtcctc tacatcggct
    12601 gaatggcgca aataacgcgc aacgtgccgt gcgatgcgcc gctggtattt gacgaccaac
    12661 gcctcgaaag ctttcgcgtc gcccgtctgc gcaagccgca cgagttccgc atcctgatcg
    12721 gcgatcacca tggccgcgcc agcatacgca agcgggcaat ccggcgcaat cgcgcccact
    12781 gcctcggtgt ggcttactta tgcggctcca tcgctagcgg tcggatatcg gttaattcag
    12841 gaatgcggag tatttactgc catttccttg ctaagcaaag gactctgccg gagcggttcg
    12901 gtttcatatt tttttcgcgc tattggcatc aaaaaaagta accgggcgcg aacctccggg
    12961 tcagagattg cgctgaggcc aatcagcctt acgcaggctt tttactttca acttttctca
    13021 aaaaggcaca ttaatgaaat cgctatcccg ctacatcgct ggcgccgtcc tggcagccgc
    13081 tagctcgttt gcatttgccg ccgatatcgt cgatacagca gtttccgctg gccagttcaa
    13141 gacgctggtc aaggcggttc aggaagccgg gttggtcgat accctcaaga gcaaggggcc
    13201 attcacggtt tttgctccga ccgatgaagc gtttgccaag cttcccgccg gcacgttgga
    13261 tgccctgctg aaggacaagc agaaactggc cgccgtcctg acttaccatg ttgttccggg
    13321 cagggtcatg gcagctcaag ttaagccggg ccaggtcaaa acagttgaag gtcagtcgct
    13381 cacggtgacc accatgaacg gtgcggtcat ggtcaacaac gccaaggtac tgaagaccga
    13441 tatcgtcgct tccaacggcg tgattcacgt aattgacacc gtagttctgc cgcagtaacc
    13501 caagttcgct ggtcacccac ccggcatttg ccgggtggcg ttgtttatag gacgctgcga
    13561 ggctcgttcg ttctccccgc tactgcgttg ccagcctgcg cacgatttcg gtcatgactt
    13621 cggtcccgaa tatcacatta tcgatggaga cgcgttcatc gttgccgtgc acaccgccga
    13681 gatccttcgg catcagcttg aacggcatga agccatagca cggtataccc ttctcccgga
    13741 aataatggca atccgtaaac ccggtaatca tcggcgcggt caccaacgtt ccgggatcgt
    13801 atacgttcgc gacctcacgg agcacgtcaa agaactcgct gtcgacggga gaagtgctgg
    13861 gcgggaaaga caggacaggt tcaatccgga tggaatcatc ggcaatgaca tttttcagcg
    13921 cgcgcaagaa tcgctgtgga tcttcgcttg gcagcaatct cacatcaagc tgtgcggacg
    13981 cttccggcgg aatgacattg accttgttgg aaccttccag catggtgatg gagatggtgt
    14041 tgcggaccat ggcgttcgcc cgcaagtcct tcgtgaattc ggtggcgaac acaggatcca
    14101 gtaatgcctt gcgcaaatcc ttcaggcgtt cccgccgctc cgccacaggg tccaggtggg
    14161 ccgtctgcgc gtagaacttc tgcacgatat cttccacttt cagcggcgtc ttgtaggtgg
    14221 cgacacggtg cagtgcttca accagcttga ggactgctga atcaggacgc ggcgcagagc
    14281 catgacctgg cgtgccggtg gcggtgagct tcaaccagaa cggcactttt tcggccggat
    14341 aggccgcgta gtaacgcacc ttgccatcgt cgccggtcag gatccaccca aattcattca
    14401 gcaccacgcc ggcgtctttg aacacatcga aatgctgt
//
LOCUS       DATMZQ010000031         7658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1060541, whole genome shotgun sequence.
ACCESSION   DATMZQ010000031 DATMZQ010000000
VERSION     DATMZQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060541"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VN496_01095"
     CDS             <1..1037
                     /locus_tag="VN496_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXF15564.1"
                     /translation="QPKQMLRLLGASGNNLKNVNLELPVGLLVCITGVSGSGKSTLIN
                     DTLYNAVAQHLYGSSAEAAPYEKIEGLEFFDKVVNVDQSPIGRTPRSNPATYTGLFTP
                     IRDLFAGVPMARERGYGAGRFSFNVKGGRCEACQGDGVLKVEMHFLPDIYVTCDVCHG
                     NRYNRETLEVQYKARNIHEVLDMTVEQAREFFSAVPVISRKLETLIDVGLGYIRLGQS
                     ATTLSGGEAQRVKLALELSKRDTGRTLYILDEPTTGLHFHDIELLLTVLHRLRDHGNT
                     VVVIEHNMDVIKTADWIIDLGPEGGDGGGSIIAQGTPETLANDASSYTGRYLSQVIGR
                     QPAPKRLAAG"
     gene            complement(1102..1494)
                     /gene="rplQ"
                     /locus_tag="VN496_01100"
     CDS             complement(1102..1494)
                     /gene="rplQ"
                     /locus_tag="VN496_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXF15565.1"
                     /translation="MRHQLGLRKLNRTTSHRLAMLRNMTNSLLRHEVIKTTLPKAKEL
                     RRVAEPIITLGKKPSLANRRLAFSRLRDREMVLKLFNELGPRFANRSGGYLRILKFGF
                     RQGDNAPMALIELLDRPEIVEEPKSAKT"
     gene            complement(1506..2480)
                     /gene="rpoA"
                     /locus_tag="VN496_01105"
     CDS             complement(1506..2480)
                     /gene="rpoA"
                     /locus_tag="VN496_01105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXF15566.1"
                     /translation="MQSSFLKPRIIDVQTISSFHAKVTMEPFERGYGHTLGNALRRVL
                     LSSMPGFAPTEVKINGVLHEYSTIDGVQEDVVDILLNLKGIVLKLHNRDEVVLNLKKQ
                     GENVVTAGDIEASHDVEIINPEHVIAHIAPGGKLDMQIKVEGGRGYVPATTRALAKEG
                     RTIGNIMVDASFSPVKRVSYAVESARVEQRTDLDKLIMDIETNGVVEPEEAIRYAARI
                     LVDQLSLFADLKGTPAPIEQPKAPQVDPILLRPVDDLELTVRSANCLKAENIYYIGDL
                     IQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPVGLEKV"
     gene            complement(2571..3197)
                     /gene="rpsD"
                     /locus_tag="VN496_01110"
     CDS             complement(2571..3197)
                     /gene="rpsD"
                     /locus_tag="VN496_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXF15567.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKGEKCFGDKCSIERRSYAPGQHG
                     QKNLRRSDYGTQLREKQKIRRIYGMLERQFRNTYKLAARAKEVTGEALLQMLESRLDT
                     VAYRMGFGSSRTEARQVVRHNGILVNGKRVNIPSYQVRPGDAVEVVEGAKAQLRIKGA
                     VAAAEGRGFPEWIEVDTKALKGTFKAKPQRSELPSTINESLVVELYSK"
     gene            complement(3214..3612)
                     /gene="rpsK"
                     /locus_tag="VN496_01115"
     CDS             complement(3214..3612)
                     /gene="rpsK"
                     /locus_tag="VN496_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXF15568.1"
                     /translation="MAKATTPAVRVRKKVKKNVAEGIAHIHASFNNTIVTITDRQGNA
                     LSWATSGAAGFKGSRKSTPFAAQIAAEQAGRAAQECGVKNLEVRIKGPGPGRESAVRA
                     LNGVGFKITSISDVTPVPHNGCRPPKKRRI"
     gene            complement(3637..4023)
                     /gene="rpsM"
                     /locus_tag="VN496_01120"
     CDS             complement(3637..4023)
                     /gene="rpsM"
                     /locus_tag="VN496_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXF15569.1"
                     /translation="MARISGVNIPNHQHAVIALTSIYGIGRARSRAICTAAGINGSIK
                     IKDLSDNDMEKLREQVAKFTVEGDLRREVTMNIKRLMDLGCYRGTRHRRGLPARGQRT
                     RTNARTRKGPRKAIQRTGGATPPPAK"
     gene            complement(4066..4179)
                     /gene="rpmJ"
                     /locus_tag="VN496_01125"
     CDS             complement(4066..4179)
                     /gene="rpmJ"
                     /locus_tag="VN496_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXF15570.1"
                     /translation="MRVQASVKKMCRNCKIVRRNRVVRVICTDPRHKQRQG"
     gene            complement(4225..4443)
                     /gene="infA"
                     /locus_tag="VN496_01130"
     CDS             complement(4225..4443)
                     /gene="infA"
                     /locus_tag="VN496_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXF15571.1"
                     /translation="MAKEETIQMQGEILETLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVDLTPYDLTRARIVFRAK"
     gene            complement(4453..5748)
                     /gene="secY"
                     /locus_tag="VN496_01135"
     CDS             complement(4453..5748)
                     /gene="secY"
                     /locus_tag="VN496_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXF15572.1"
                     /translation="MGKFGDLKRRLLFLLGALIVYRIGAHVPVPGIDPIQLAELFKSQ
                     KGGILDMFNMFSGGALSRFTIFALGIMPYISASIIMQLLTVVSPTLEQLKKEGESGRR
                     KITQYTRYGTLFLATFQGLGIAIALESQRGLVIDPGLAFRFTTVVTLVTGTMFLMWLG
                     EQITERGIGNGISLIIFAGIAAGFPRAIGGTLELARTGAFSIPLVLLLLVGAILVTGF
                     VVFVERGQRKILVNYAKRQVGRKVYGGQSSHLPLKLNMSGVIPPIFASSIILFPATLA
                     GWFGSNESMTWLRDISGTLHPGQPIYVLLYASAIIFFCFFYTALVFNPKETAENLKKG
                     GAFVPGIRPGDQTAKYIEKITMRLTLAGAIYVTLVCLLPEFLIVKWNVPFYFGGTSLL
                     IIVVVTMDFMAQVQAYIMSHQYESLLKKANFKGGMFPTR"
     gene            complement(5775..6209)
                     /gene="rplO"
                     /locus_tag="VN496_01140"
     CDS             complement(5775..6209)
                     /gene="rplO"
                     /locus_tag="VN496_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXF15573.1"
                     /translation="MRLNQIKPAAGAKHAKKRVGRGIGSGLGKTAGRGHKGQKARSGG
                     FHKVGFEGGQMPLHRRLPKRGFISLSRGDTAEVRLSSLDKLPVDEIDIAVLKQAGVVP
                     HAALSAKVILNGEIKRKVKLRGLLVSKGARSAIEKAGGSVEG"
     gene            complement(6210..6419)
                     /gene="rpmD"
                     /locus_tag="VN496_01145"
     CDS             complement(6210..6419)
                     /gene="rpmD"
                     /locus_tag="VN496_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXF15574.1"
                     /translation="MVKKTETAKKSGKTVKVTLVRGVVGTRESHRATVRGLGLRRINH
                     TVELEDTPAVRGMINKISYLVKCEA"
     gene            complement(6435..6947)
                     /gene="rpsE"
                     /locus_tag="VN496_01150"
     CDS             complement(6435..6947)
                     /gene="rpsE"
                     /locus_tag="VN496_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXF15575.1"
                     /translation="MARMQQQNEERSDGMREKMVSVNRVTKVVKGGRILGFAALTVVG
                     DGDGGIGMGKGKAREVPVAVQKAMDEARRKMVKISLKNGTLQHAVVGEHGSAKVFMQP
                     ASEGTGIIAGGPMRAIFEVMGVTNVLAKCLGSTNPYNVVRATINGLQGMSTPSEIAAK
                     RGKTVEQILG"
     gene            complement(6959..7309)
                     /gene="rplR"
                     /locus_tag="VN496_01155"
     CDS             complement(6959..7309)
                     /gene="rplR"
                     /locus_tag="VN496_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXF15576.1"
                     /translation="MDKKQSRMRRARQTRAKIAELKAVRLAVHRSNDHIYAQVIDPTS
                     KVLASASTLEADVRKEVGNGRNLKAAAIVGKRIAEKAKAQGVEQVAFDRAGFKYHGRV
                     KALAEAAREAGLKF"
     gene            complement(7327..>7658)
                     /gene="rplF"
                     /locus_tag="VN496_01160"
     CDS             complement(7327..>7658)
                     /gene="rplF"
                     /locus_tag="VN496_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXF15577.1"
                     /translation="RALVANMIEGVTKGFEKKLQLVGVGYRAQAQGDKLNLTLGFSHP
                     VVHRMPKGIKVETPAQTEIVIKGIDKQVVGQVAAEVRGYRPPEPYKGKGVRYSDERVV
                     LKETKKK"
BASE COUNT         1505 a         2322 c         2044 g         1787 t
ORIGIN      
        1 ctcaacccaa acagatgctg cgactgctgg gggcaagcgg caataatttg aagaatgtga
       61 atttggaact tccggtggga ttgctggttt gtataacagg cgtttccggg tcgggcaagt
      121 ccacactcat caatgacacc ctctataacg cggtggcgca gcatctgtat ggcagcagcg
      181 cagaagcggc cccatacgaa aaaatcgagg gactggaatt tttcgacaaa gtcgttaacg
      241 tggaccagag tccgatcgga cgcacgccgc gttccaaccc cgccacgtac accggcctgt
      301 tcacaccgat ccgcgatctg ttcgcgggcg tgccgatggc gcgcgagcgc ggctacggag
      361 caggccgttt ttccttcaat gtcaaaggtg gacgttgcga agcgtgtcaa ggcgatggcg
      421 tgctgaaggt ggagatgcac tttctgccgg acatctatgt aacctgcgat gtttgtcacg
      481 gcaatcgcta caaccgtgag actttggaag ttcagtataa agcccgcaat attcacgagg
      541 tacttgacat gaccgtggag caggcgcggg agtttttcag cgctgttccc gtcatttctc
      601 gtaagctcga aacactgatc gatgttggac ttggctatat ccgcctaggc cagagcgcga
      661 ccaccctgtc cggcggcgaa gcgcagcgcg tcaagctcgc actcgagcta tccaaacgcg
      721 ataccgggcg cacactctac atcctcgacg aaccgaccac tggccttcat ttccacgaca
      781 tcgaattgct gcttaccgtg ttgcaccgct tgcgcgatca cggcaacacc gtggtggtga
      841 tcgagcacaa tatggacgtc atcaagacag cggactggat catcgatctc ggtccagaag
      901 gcggtgacgg gggcggcagc atcattgctc agggcacacc cgaaaccctt gcgaacgacg
      961 cgtcctcgta tacgggccgc tatttaagcc aagtgatcgg tcgccagccg gcaccaaagc
     1021 ggctcgctgc cggctagcag gcagaaaaaa aagccgggca agcccggctt tttcagaccc
     1081 gcgattcatt gcagttctat atcaggtctt cgcgctcttg ggttcttcca cgatttccgg
     1141 acgatcaagc aattcgatta atgccatggg agcgttatcg ccctgccgga aaccgaattt
     1201 cagaatgcgc aaataacctc cgctgcgatt cgcgaaacga ggccccagtt cattgaacag
     1261 cttgagcacc atttcacgat cacgcaagcg ggaaaatgcc aaacgtcgat tggccagaga
     1321 gggttttttg ccaagcgtaa tgatcggctc ggccacgcgg cgaagttcct tggccttcgg
     1381 gagcgtggtc ttgatgacct cgtgccgcag caatgagttt gtcatgttac gcagcatggc
     1441 cagccgatgg ctggtcgtgc ggttcagttt tcgcaatccg agttggtggc gcatgctcta
     1501 tccccttaca ccttctcaag tccgaccggc ggccagttct cgagcttcat ccccagtgtc
     1561 agcccgcgcg aggccagaac ttccttgatc tcgtttagcg atttccggcc caggttcggt
     1621 gtcttcagca attcagtttc cgtgcgctgg atcaggtcgc caatgtaata gatgttttca
     1681 gccttgaggc agtttgccga acgcactgta agctcgagat catccaccgg gcgcagcaga
     1741 atgggatcga cctggggcgc ctttggttgc tcgatcggcg ccggtgtccc tttcaggtcc
     1801 gcgaacagtg acaactgatc caccagtatg cgggcggcgt agcgaatggc ctcttccggc
     1861 tcgaccacgc cgttggtctc gatatccatg atcagcttat ccaggtcagt gcgctgttcg
     1921 acccgagccg attcaacagc ataactaacc cgcttcacgg ggctgaaaga ggcgtctacc
     1981 atgatattgc cgatggttcg gccttctttg gccaatgcgc gcgtcgtagc gggcacgtaa
     2041 ccgcggccgc cttcgacctt gatctgcatg tccagcttgc cgcctggggc gatgtgcgca
     2101 atgacatgtt ccgggttgat gatctccacg tcgtgtgacg cttcaatgtc gcccgcggta
     2161 accacgttct ctccctgctt cttgagatta agaacgacct catcgcgatt gtgtaatttc
     2221 agcacgatgc ccttcagatt aagcaggata tcgacgacgt cctcctgtac tccatcgatg
     2281 gtggagtatt catggagtac gccgttgatc ttgacttcgg taggtgcaaa accgggcatc
     2341 gaggacagca gcacccggcg cagggcatta ccgagggtgt gaccataacc gcgttcgaac
     2401 ggctccatcg taaccttcgc atggaaggac gagatcgttt gtacatcgat gattcgcggc
     2461 ttcagaaagc tgctttgcat aaacgtgacc ctctagattg aattcgggac gtccgtatta
     2521 aaaacacgcg gcccggccgg tccagcggcg ggccgcatta ccgtgttgcc ttacttcgaa
     2581 tacagttcca ccaccagcga ttcgtttatc gtggaaggca gctcagagcg ttgtggcttt
     2641 gccttgaaag tacctttgag cgctttggta tcgacttcga tccactcggg aaagccgcgg
     2701 ccttccgccg ccgccacggc gcccttgatg cgcaactggg ctttcgcgcc ctcgaccacc
     2761 tccacagcat ctcccggccg cacctggtat gacgggatgt tgacccgctt gccattcacc
     2821 agaatgccgt tgtggcgcac cacctgacgt gcctcggtac gcgaggaacc gaatcccatc
     2881 cgataggcga cggtatcgag ccggctttcc agcatctgca agagcgcctc gcccgttact
     2941 tccttcgcgc gcgcggcgag cttgtaggta ttgcgaaact ggcgttccag cattccatag
     3001 atgcgacgga tcttctgctt ttcgcgcagt tgcgtgccgt agtcggaacg gcgcaagttc
     3061 ttttggccat gctgaccggg cgcatacgac cggcgctcga tcgagcactt gtcgccgaaa
     3121 catttttcgc ctttgagaaa cagtttttca ccttctcggc ggcactggcg gcacttcggg
     3181 tctaaatttc ttgccacagc aatctccttg ggatcagata cggcgcttct tcggcggacg
     3241 gcatccgttg tgcggaaccg gggtcacgtc cgagatgcta gtgatcttga aaccgacacc
     3301 attcagagcg cggacagcgg actcgcgacc cgggcccggg cctttgatgc gcacttccag
     3361 attctttacg ccacactcct gggcagcgcg cccagcctgc tctgcagcga tctgggctgc
     3421 aaacggggtc gacttgcgtg agcccttgaa accagccgcc ccagaagttg cccaggacag
     3481 tgcgttcccc tggcgatcgg tgatggtcac aatcgtgttg ttgaaggagg cgtgaatgtg
     3541 ggcgatgccc tcggccacgt tcttcttgac cttcttgcgt acccgtactg ctggggtggt
     3601 tgcctttgcc atatcggaaa tccttgctcg gttcgtttac ttcgctggtg gcggagttgc
     3661 accaccggta cgctggatcg ccttgcgtgg ccccttgcgg gttcgtgcat tggtgcgcgt
     3721 gcgctgtcca cgtgccggca atccgcggcg gtgccttgtc ccgcgatagc agcccaggtc
     3781 catcaggcgt ttgatattca tcgtgacttc acggcgaaga tcgccttcga ccgtgaattt
     3841 cgcgacctgc tcacgcagct tttccatgtc gttgtcggat agatccttga tcttgatcga
     3901 accgtttatg ccggccgcag tgcaaatggc gcgcgaacgg gcgcgaccga tgccatagat
     3961 cgacgtcagc gcaatgactg cgtgctgatg gttgggaatg tttacacctg agatacgggc
     4021 catatgcgtt cctgcgttct gtatttaaag tcgcgttacc gcgcgttaac cttggcgttg
     4081 cttatggcgc gggtccgtac agatcacgcg cacaacgcga ttgcgccgga cgatcttgca
     4141 gttccggcac atcttcttta ccgatgcctg tactctcatt tctgctcctt cagtgcgcaa
     4201 tgtcgcgagc ttgttttatc cagactactt cgcgcggaac acgatgcgcg cgcgtgtcaa
     4261 atcgtaaggc gtaagatcaa ccgtaacctt gtcacccgga agaatccgga tatagtgcat
     4321 ccgcattttt cccgaaatat gtcctagcac cacatggccg ttttccagct tcacgcgaaa
     4381 cgtcgcgttt ggcagggttt ccaggatttc tccctgcatc tggatagtct cttccttggc
     4441 catgctcctc cgctatcgag tcggaaacat cccgcccttg aaattcgctt tcttgagcag
     4501 gctctcatat tgatgcgaca tgatgtaggc ctgtacttgg gccatgaaat ccatagtcac
     4561 caccacgata atcagcaaac tggtgccacc gaaataaaac ggcacattcc acttcacgat
     4621 caaaaattcc ggcaacaggc acacgagcgt cacgtagatc gctccagcga gcgtcaacct
     4681 catggtgatc ttctcgatgt acttcgctgt ctggtcgcca ggccgaatac ccggtacgaa
     4741 agcgccgccc ttcttgaggt tctccgccgt ttccttggga ttgaacacca gggccgtgta
     4801 aaagaagcag aagaaaatga tcgcggacgc gtaaagcaat acgtaaatcg gctggcccgg
     4861 gtgcagcgtt ccgctgatat cgcgcagcca ggtcatgctt tcgttactgc caaaccaacc
     4921 ggccagcgtg gccggaaata aaatgatgct cgatgcaaat atgggcggta tcacccctga
     4981 catgttcagt ttcaggggca gatgcgaact ctggccgcca tatactttgc gtcccacctg
     5041 ccgcttcgca taattcacca ggatcttgcg ctgaccccgc tctacgaaca ccacaaatcc
     5101 agtgaccagg attgcgccga ccagcaacag cagcaccagg ggaatggaaa acgccccggt
     5161 acgcgccagt tccagcgtgc ctccgattgc gcgcggaaag ccggcggcga tgccggcaaa
     5221 aatgatgagc gagataccgt tgccgatgcc gcgctccgtg atctgttcgc cgagccacat
     5281 caggaacatc gtgccggtta ccagggttac aaccgtggtg aagcgaaacg ccagtcccgg
     5341 atcgattacc aggccgcgtt gcgattccag cgcgattgcg atgcccagcc cctgaaacgt
     5401 cgccagaaac aatgtcccgt agcgcgtgta ctgcgtgatc tttctgcggc cggactcgcc
     5461 ttccttcttc aactgctcca gtgtcggcga tacgaccgtc agcagctgca tgatgatcga
     5521 tgccgaaatg tacggcatga tgcccagcgc gaaaatggta aatcgtgaca aggcgccgcc
     5581 tgagaacata ttgaacatgt ccaggatgcc gcccttctgc gacttgaaca gttccgccag
     5641 ctgaatcgga tcgatcccgg gcacagggac gtgcgcaccg atgcggtaca cgatcaacgc
     5701 gcccagcagg aacagcaatc ggcgcttgag gtcgccgaat ttgcccatac cgattaaaga
     5761 tttttctgcc aaatctagcc ttccacgctt ccgccggctt tttctatcgc cgaacgcgca
     5821 cctttgctaa ccagcaatcc gcgcagcttc accttgcgct tgatctcacc attcaatatg
     5881 actttggctg acagcgccgc atgcggcacc acacctgcct gcttcagtac tgcaatgtca
     5941 atttcatcta ccggcaactt gtcgagcgac gacaatctca cctcggccgt atcaccccga
     6001 ctgagcgaaa tgaatccacg ctttggcagc cgccgatgca gcggcatttg gccgccctcg
     6061 aagccaacct tgtgaaagcc gcccgaacgc gccttttgtc ccttgtggcc acgcccggcc
     6121 gtcttgccga gtccactgcc gatgcctctg ccgacgcgct ttttcgcgtg cttggctccc
     6181 gccgcgggtt taatttggtt caaacgcatc taggcctcgc acttcaccag ataacttatc
     6241 ttgttgatca tgccgcgcac cgcgggagta tcttccaact caacggtgtg gttaatgcgc
     6301 cgcaagccaa ggccgcgcac ggtggcgcga tggctttctc gcgtgccaac gacgccgcgg
     6361 accagcgtga ccttgacggt cttgccggat ttttttgcgg tttctgtctt tttgaccatg
     6421 attgtcgtaa accgttagcc gagtatttgt tcaactgtct tgccgcgctt ggcggcgatt
     6481 tcggaaggcg tactcatgcc ttgcaatccg tttatcgttg ctcgcaccac gttgtacgga
     6541 ttggtcgaac ccagacattt ggccagtaca ttagtcactc ccatgacttc aaaaatcgcg
     6601 cgcatgggcc ctcccgcgat gatcccggtg ccttccgatg ccggctgcat gaagactttc
     6661 gccgacccgt gttcgccgac gactgcatgt tgcaacgtcc cgtttttcag gctgatcttg
     6721 accattttgc ggcgcgcctc gtccatggcc ttttgcaccg ccaccggcac ttcccttgcc
     6781 ttgcccttgc ccatgccgat tccgccatct ccatcgccca ctacagtcag agcggcaaag
     6841 cccaggatac ggccgccttt aaccacctta gtcacgcgat tcactgacac catcttttcg
     6901 cgcatgccgt cgctgcgctc ttcgttctgt tgctgcattc tggccatggt tgatatcctt
     6961 agaacttcag tccggcttcg cgggccgctt cggcaagcgc cttgacacgg ccgtgatact
     7021 tgaatccggc gcgatcaaat gcgacctgct caacaccctg cgccttggcc ttttcagcga
     7081 tacgcttgcc cacgatggcg gcggccttga ggttccttcc attaccgact tccttgcgca
     7141 catcggcctc cagcgtcgat gccgatgcga gcactttgga ggtggggtcg attacttgcg
     7201 catatatatg gtcattggag cgatgcactg caagcctcac cgccttcaat tccgcgattt
     7261 ttgcgcgggt ctggcgtgca cggcgcatgc gcgattgctt cttgtcaatc atggtcgtcg
     7321 gctccgttac tttttcttgg tttctttcag tacaacccgt tcgtcggaat agcgaacgcc
     7381 cttgcccttg taaggctcgg gtggacgata gccgcgcact tccgccgcta cctggccgac
     7441 gacctgcttg tctatgcctt tgatcactat ttcggtctgc gccggcgttt ccaccttgat
     7501 gcctttcggc atccggtgca ccacggggtg cgaaaacccc agcgtcagat tcagcttgtc
     7561 gccctgagcc tgcgcgcggt atccgactcc aaccagttgc agctttttct caaatccctt
     7621 tgtcacgcct tctatcatgt tcgccaccaa cgcccgca
//
LOCUS       DATMZQ010000032         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_370059, whole genome shotgun sequence.
ACCESSION   DATMZQ010000032 DATMZQ010000000
VERSION     DATMZQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370059"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VN496_01165"
     CDS             <1..1095
                     /locus_tag="VN496_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF15578.1"
                     /translation="YDPANAEAGRKGAVSKGALRGLRTSAKKMNPALAADSGSMADLS
                     WQNFIKEVFFDSETSIGLISTPPGPYPQEAVVPPREMTHIRDEINRVTQSRRMLAHGL
                     VTPQLGRADLDFMQMQAETLKVDAWKAYTGSCPKGFEHGWFLDDEKIAYPMLEKARSL
                     DIKRLCVHKGLPLGTVADYNHPRDLIKAAKDFPDIAFLCYHSGLLGPTAPKASGEVPW
                     TTEFCEMKKKQPGINNIYMELGSTFGQLVTTNPTACAHLLGQLVAAFGADHVLWGTDS
                     IWYGTPQWQIEAFRRFQIPDALIDKHGYASLTPEVKEQIFGLNAARVFNVDVNAKRNE
                     IPADYLSQIKMAYLDEGASPSHRWYGWVAA"
     gene            complement(1129..1482)
                     /locus_tag="VN496_01170"
     CDS             complement(1129..1482)
                     /locus_tag="VN496_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15579.1"
                     /translation="MRYTRPEFSAALTAPIMDTWYSKDIGDGLTAYEPCGEIEAAFAP
                     LFAATGKPAGMAVFTRYDSEGHLQCRVTAFFSPAATPLARLFGAAPCARPARDGLTLL
                     CGEERCWSTLFARHA"
     gene            1565..>2490
                     /locus_tag="VN496_01175"
     CDS             1565..>2490
                     /locus_tag="VN496_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF15580.1"
                     /translation="MYQRYFGFVEAPFSIAPDPRYLYLSQRHQEALAHLLYGINGNGG
                     FVLLTGDVGAGKTTVCRCLLEQIPPSCDVAYIFNPKLTVEELLSTICIEFGIACPPGT
                     PSIKMYVDCINAYLLEAHASGRHTVLIIDEAQNLSADVLEQMRLLTNLETNQRKLLQI
                     ILIGQPELATMLERPELRQVSQRIIARYRLGPLSKPEVAAYVTHRLEIAGARRPLFSP
                     RLTGQLYRLTRGVPRVINVLCDRALLGAYVQGKERVDAATLTQAAREVFHQADDRRNV
                     LRGLIATLILTAGVAVAFTLHRQDMNDARMSIP"
BASE COUNT          529 a          743 c          721 g          497 t
ORIGIN      
        1 tacgaccccg ccaatgcaga agctgggcgc aaaggtgcag tcagcaaagg cgcattgcgc
       61 ggcctgcgca ccagcgccaa aaaaatgaac cccgcgctgg cggccgatag cggttccatg
      121 gcggatttgt catggcagaa cttcatcaag gaagtgttct ttgacagcga gacgtcgatc
      181 gggctgattt cgacacctcc cgggccgtat ccgcaagaag cggtagtccc gcccagggaa
      241 atgacgcata tccgcgatga gatcaaccgt gtcacgcaat cgcgtcgcat gcttgcccac
      301 ggcctcgtca cgccgcagct tggccgggcc gacctggatt tcatgcagat gcaggccgaa
      361 acattaaagg tagatgcgtg gaaggcttac accggctcct gtccgaaggg attcgaacac
      421 ggctggtttc tcgacgatga gaaaattgcc tatccgatgc tggaaaaagc ccgcagtctg
      481 gatatcaagc gcctgtgcgt ccacaagggg ttgcctttgg gcacggtggc cgattacaac
      541 catccgcggg atctcatcaa ggcggcgaag gattttcccg acatcgcctt tctttgttac
      601 cactccgggt tgctcgggcc gactgcgccc aaagcatcag gtgaagtacc ttggacaacc
      661 gaattttgcg agatgaagaa aaagcagccc ggcataaaca atatctatat ggaactgggt
      721 tcgaccttcg ggcaattggt cacgaccaat cccactgctt gcgcgcacct gcttggccag
      781 cttgtagccg cattcggtgc ggatcacgtg ttgtggggaa ctgattcaat ctggtatgga
      841 acgccgcagt ggcagatcga agccttccgc cgtttccaga ttcccgatgc attaatcgac
      901 aagcacggct atgcgtcgct gacacctgaa gtcaaagaac agattttcgg tctgaatgcg
      961 gcgcgcgtat tcaatgtaga cgtcaacgca aagcgcaacg aaattccggc ggattatttg
     1021 agccagatca agatggccta tcttgacgaa ggcgcttcac cgagtcaccg ctggtacggg
     1081 tgggtggccg cctgaaactg caaacgccgc tgtccggtaa cgcaacgatc aggcgtgcct
     1141 tgcaaataac gtcgaccagc agcgttcctc gccgcacagt aatgtcagtc cgtcccgtgc
     1201 gggtcgcgcg cacggggcgg caccaaacag gcgcgccagc ggcgttgcgg cgggtgaaaa
     1261 aaacgcggtg acccggcatt gcaggtgtcc ttcggagtcg tatcgagtga acaccgccat
     1321 gcccgctggt ttgcccgttg ctgcgaacag cggcgcaaag gcggcctcga tttcgccgca
     1381 cggctcgtac gccgtcagcc cgtcgccaat atctttggaa taccaggtat ccatgatcgg
     1441 tgcggtcaga gcagcactga actccggtcg agtgtaacgc aagtcatgaa atgccggcgc
     1501 ggcgcatcgg gcggtagcgg tccgccgcta taatgacgcg gattcttcag ccgccaggtg
     1561 gtccgtgtac caacgttatt tcggcttcgt cgaagcaccg ttctcgatcg cacccgaccc
     1621 gcgctatctg tacctgagcc agcggcacca ggaggcgctc gcgcatctgt tgtatggaat
     1681 caacggcaac ggtggattcg tcctgctcac gggcgacgtt ggcgcgggca aaacgaccgt
     1741 atgccgttgc ctgctcgaac agattccgcc gtcctgcgac gtcgcttaca ttttcaatcc
     1801 caagctcacc gtcgaggagc tgctctcgac catctgcatc gaattcggta ttgcttgtcc
     1861 gcccgggacg ccgagtatca agatgtacgt tgactgcatc aacgcctatc tgctcgaagc
     1921 acacgccagc ggccggcata cggtgctgat catcgacgag gcgcagaatc tgtctgccga
     1981 tgttctggaa cagatgcgcc tgctcaccaa tctggagacg aaccagcgca aattgctgca
     2041 gatcatcctg atcggccagc ccgaattggc cacgatgctg gagcggcccg agctacggca
     2101 ggtgagccag cgaatcatcg cgcgctatcg cctcggaccg ctttccaaac ccgaggtcgc
     2161 agcgtacgtc acgcaccgac tcgaaatagc gggcgcgagg cgtccgttat tttccccgcg
     2221 attgacgggg caactatatc gtctcacgcg cggcgttcca cgcgtgatta acgtactgtg
     2281 cgatcgcgct ctgctgggcg cttacgtgca aggcaaggaa cgcgtcgatg ccgctacgct
     2341 aacgcaagcg gcgcgtgaag tgtttcacca ggccgacgac cgccgcaatg ttttgcgcgg
     2401 actcatcgca actttgattc tcacggccgg tgtcgccgtg gcatttaccc tgcatcggca
     2461 ggatatgaat gatgcgcgca tgtccatccc
//
LOCUS       DATMZQ010000033         6227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2787168, whole genome shotgun sequence.
ACCESSION   DATMZQ010000033 DATMZQ010000000
VERSION     DATMZQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787168"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1091
                     /locus_tag="VN496_01180"
     CDS             183..1091
                     /locus_tag="VN496_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXF15581.1"
                     /translation="MRHIVRAAFAIIAFGACAAASAQDIEPRAYSNAPVGVNFLIVGY
                     ANTRGDLSFETLPLTNAELHASSAVFAYARALDLWGKSGKFDVIAPYTWLSGTADFAG
                     QGVQRDVNGFNDPRFRLSVNLYGAPALTREEFRQYKQDLIIGASLQVSPPLGQYDDTR
                     LINLGTNRWSFKPEIGISKARGPWTLEFAAAATLYTTNNDFFGGNTRSQDPLYSMQGH
                     VIYGFSSGIWASLDATYFTGGRSTLNGTINNDLQQNWRTGATLAVPVNAYQSIKFYAS
                     SGVSARTGNNYDLLGVAWQVRWGGGL"
     gene            1141..2562
                     /gene="nhaA"
                     /locus_tag="VN496_01185"
     CDS             1141..2562
                     /gene="nhaA"
                     /locus_tag="VN496_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HXF15582.1"
                     /translation="MKNFNNENPESMPKIRVARLDERYPLEPLFGRIFSPFEQFLRRT
                     TAGGIVLIATTIAALVLATALGADAIHRFTEQPLALFTSEWWSLGLSVHEWVNDGLMT
                     FFFLLVGLELKREILVGELSSLKDAALPVVAALGGMVLPALCYAVFNAGTPAAAGWGI
                     PMATDIAFAVGILVLLAWRVPKNLIIFLTALAIADDLGAVLVIAVFYTADINVYMLAA
                     AASLLGLLVLFNRAGIRHPLAYAILGVLLWFAVHTSGVHATLSGVLLAMAIPARPAYT
                     PAHFERRIDELQSAFRANRRDKNSTDDPLSNQRMAAIAHAMERSAVAVQSPLQRIEHG
                     LTPWVTFLIVPVFALSNAGIDLFSITWSEALASKITLGVISGLLVGKFAGITLFSWIA
                     IRVGIARLPAGVSWTQLMGAAWLAGIGFTMSLFIAQLALTTPEAIDQAKLGILLGSSI
                     SAVIGLLWLYLHSRQHSERPRDA"
     gene            complement(2568..2729)
                     /locus_tag="VN496_01190"
     CDS             complement(2568..2729)
                     /locus_tag="VN496_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15583.1"
                     /translation="MASGASLVEINGSCKAFHTRGAGAHDPARYGLPITVVQLAVSAL
                     YVLVLSRLM"
     gene            2868..5231
                     /locus_tag="VN496_01195"
     CDS             2868..5231
                     /locus_tag="VN496_01195"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXF15584.1"
                     /translation="MSQQQIVLPTDRSVLPIPEPRIAHSTVLNVRNATLPPRFEVKAP
                     AGAPNVLIVLIDDMGFGQSSSFGGPIHMPTVERLAKEGLRYNQFHTTALCSPTRSALL
                     SGRNHHMNNFGSISETGTAFPGQTGQRPNNIAPLAEMLRLNGYSTAAFGKNHETAAWE
                     VSPSGPTDRWPTRQGFDKFYGFMGGETNQWAPLIYDGMTQVEPPHDPNYNFMTDMTNQ
                     AVAWVQYQKSLTPEKPFFVYFAPGATHAPHHVPKEWIAKYKGKFDQGWDKLREETLAR
                     QIKLGVVPAGTKLAAKPAAITDWDKLSADEKKLFARQMEVFAAFGEYADTEIGRLVDA
                     IGSMDQLDNTLIFYIVGDNGASAEGGMHGLVNEYTYFNQVPESVVDILKHYDELGGPK
                     TFPHYAAGWAVAGDTPFEWTKQVASNYGGTRNGMVVHWPKVIQAKGEVRSQWHHVIDI
                     APTVLEAASLPEPKSVNGTVQEPIEGVSMLYSFADAKAKDRHTTQYFEIFGNRAIYKD
                     GWLAGTVHRAAWEYKVRAPLENDIWELYNTREDFSLANDIAAQNPDKLKEMQELFLIE
                     AVKYSVLPLDDRLTERFIAGMVGRPDLMAGRTSLTVHEGMIGMSENVFINMKNRSHTI
                     TAEVQVPKQGASGAIMAQAGRFGGWSLYMQDGKPMYTYNWLGLQRFTVASNTPLSGGK
                     ATIRFEFAYDGGGMGKGGTGTLFVNGKQVATGRIERTQCCVFSADEGTDVGADEGTPV
                     TEAYKVPFKFTGTIDKVTIELKEMKLADKGDAEQAHKEARLRKGVSD"
     gene            complement(5382..5888)
                     /locus_tag="VN496_01200"
     CDS             complement(5382..5888)
                     /locus_tag="VN496_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15585.1"
                     /translation="MRKIGKIIATEFREILPATILFLFLFHMIALTRAVSLNDFSFTA
                     LRATSATLGALIVAKAILLIEAFPLVRVSPSLLFTRVLWKTLLYGAVVIIFKFIEELV
                     PLLLKHGNVRDAIQAVFSEVAWPLVSVMALWTLGGLVLYSLASELGSVIGPQRVKEML
                     LSRVNSVP"
     gene            complement(5890..>6227)
                     /locus_tag="VN496_01205"
     CDS             complement(5890..>6227)
                     /locus_tag="VN496_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15586.1"
                     /translation="IGVAFPLIRSIPATYGYIVRRRVFILYGELKFLEAELAARTSDA
                     KAVKELLERVGRLEARANKLQVPFMYSHFLYTLRAHIDFVRARLMKQNPESAALPTVI
                     AGSAAMRSN"
BASE COUNT         1380 a         1782 c         1757 g         1308 t
ORIGIN      
        1 ctatatcgat aaaaagctga agaacgatac cgtgcgcaaa aagcttctgc ggccggctaa
       61 ttaagtccct cgcgacgcat tgccgcgctg gccgttgcat ttaaccgcgc cccgtggaga
      121 aaatgattac aaaggcgcca gggatcatag gcagcaccgc tgcgtcagcc tgcatgtttg
      181 atatgcgaca cattgttcgc gcggcattcg caataatagc tttcggcgct tgtgcagcag
      241 cgagcgcaca ggacatcgag ccgcgcgcct attccaatgc gccggttggc gtaaatttct
      301 tgattgtcgg ttacgcgaat acgcgcggcg acctgtcgtt cgagaccttg cctctgacca
      361 atgctgaatt gcatgcctcc agcgccgtat tcgcttacgc aagggcgctt gatttatggg
      421 gcaagtcagg caagttcgac gtcatagcgc cttacacctg gttgtccgga acggcggatt
      481 ttgccgggca aggggttcaa cgcgatgtaa atggcttcaa tgatcccagg tttcggctgt
      541 ccgtcaatct ctatggggcg ccagcgctca cccgtgagga attcaggcaa tacaagcagg
      601 atctgattat tggcgcgagc ctgcaagtgt ccccgccgct gggtcagtac gacgataccc
      661 ggctgatcaa ccttggcacc aatcgctggt cgttcaaacc cgaaataggt atctcgaagg
      721 cgcgcggtcc ctggacgctg gagttcgccg cggcagcaac cttgtatacc accaacaatg
      781 atttcttcgg cgggaacacg cgttcacagg acccgctcta ctcaatgcag gggcacgtga
      841 tttacggctt ctcctcgggc atttgggctt cgctggatgc aacctacttc accggcggcc
      901 gttcaaccct gaacgggacc ataaacaatg atcttcagca gaattggcgc accggcgcaa
      961 cgctcgccgt gccagtgaat gcctatcaat ccatcaagtt ttacgcgagc agtggtgttt
     1021 cagcacgcac aggcaacaac tatgatctgc tcggtgttgc ctggcaggtt cggtggggtg
     1081 gcggtctctg acgagtgctg ctttcggttc catgaaccgc gctatcccgg cgcaatcgca
     1141 atgaagaact tcaacaatga aaatcccgaa agcatgccga aaatccgcgt agcaagactg
     1201 gacgaacgtt atccactgga accgctgttc gggcgcattt tttcaccgtt cgagcaattt
     1261 cttcgccgta ctaccgcggg cggtatcgta ctgatcgcca ctacgattgc cgcgcttgtc
     1321 cttgctacgg cactcggcgc cgatgccatc catcgattca ccgagcagcc gttggcgctg
     1381 ttcacgagtg agtggtggag tctcgggctt tcggtgcatg agtgggtaaa cgacgggctc
     1441 atgaccttct ttttcctgct ggttggtctc gaattgaaac gtgaaatact ggtcggcgaa
     1501 ctttcgtccc tcaaagatgc cgcgctgccg gtcgtggcgg cgctcggggg aatggtattg
     1561 ccggcgttgt gttatgccgt atttaacgca ggcacgcccg ccgcagccgg ttggggcatc
     1621 ccgatggcaa cggatattgc gttcgccgtg ggcattctgg tcctgctggc gtggcgggtt
     1681 ccgaagaatc tcatcatttt cctgacggca ttggcgattg ccgatgacct cggtgcagtg
     1741 ctcgttatcg cggtttttta tacggctgac atcaatgtgt acatgcttgc cgcagccgcg
     1801 tcgctgctcg gcctgctggt actgttcaac cgcgcgggca tccgccatcc acttgcctac
     1861 gccatcctgg gcgtgttgct ctggttcgcg gtccacacat cgggtgtgca tgcgaccctg
     1921 tcaggcgtcc tgctggccat ggctatcccc gcccgccccg cgtacacacc ggcgcatttc
     1981 gagcgccgca tcgatgaatt gcaatcggcg tttcgcgcga atcgccgcga caagaactcc
     2041 accgacgacc cgctcagcaa tcagcggatg gcggccatcg cgcacgcgat ggaacgttcg
     2101 gcggttgccg tgcaaagccc gctccagcgc atcgagcatg gacttacgcc gtgggtgaca
     2161 tttctgatcg ttcctgtatt cgccctgtcc aacgcgggca tcgatctgtt cagcataacc
     2221 tggagcgaag cgcttgcgag caagattacg cttggtgtca tcagcggttt gcttgtcggc
     2281 aagtttgccg gcattacgct gttcagctgg attgcaattc gagtgggcat cgcgcgatta
     2341 ccggcgggag taagctggac acagctgatg ggagcggcct ggctggctgg aattggcttc
     2401 accatgtcgc tgtttattgc gcaactggcg ttgacgacgc ctgaagccat cgaccaggcc
     2461 aagctgggca tcctgctcgg gtcttcgatt tcggcagtga tcgggctgct ctggctttat
     2521 ctgcactcgc gccagcattc cgagcgtccg cgagacgcgt aacagtttta catcaaccgg
     2581 gacagcacca gcacatagag ggccgatacc gcaagctgca cgacggtgat gggcaagcca
     2641 taacgcgcag gatcatgagc cccagcaccg cgagtgtgga aggccttgca cgacccgttg
     2701 atctcgacca aactcgcgcc cgatgccaag tcattgggtc aagtccctgg ctaacacaag
     2761 tctaaacaca acaatgaaag gtatgcgtga aaactagcaa cgttggtcga ataattcttg
     2821 ctgtgattgg cggcagcatg ctcattgcgg cattttgtca accagtcatg tcacaacaac
     2881 agatcgtgct tccaacggac cgttcggtac tcccaatccc ggaaccgcgc attgcgcaca
     2941 gcactgtact gaacgtccgc aatgctacgc tgccgcctcg attcgaggtc aaggcgccgg
     3001 ccggcgcgcc gaatgttctg atcgtactga tcgatgacat ggggttcggc cagtcgagct
     3061 ccttcggcgg acccatccat atgccgaccg tagaacgcct ggccaaagaa gggctgcgtt
     3121 acaaccagtt ccataccacg gcgctgtgct cgccgacgcg ctcggccctg ctctccgggc
     3181 gcaatcatca catgaacaac ttcggctcca tttccgaaac aggcaccgcc tttcccggac
     3241 agaccggaca gcgcccgaac aatatcgcgc cgctcgccga gatgttaagg ctgaacggct
     3301 acagtaccgc ggcctttggc aagaaccacg aaaccgcggc ttgggaagtc agtccttccg
     3361 gcccgacaga tcgttggccc actcgccagg gattcgacaa gttctacggc ttcatgggcg
     3421 gcgagaccaa tcagtgggcg ccgctgatct atgacggcat gacgcaggta gagccgccgc
     3481 atgacccaaa ctacaatttc atgaccgaca tgaccaacca ggccgtcgct tgggtgcagt
     3541 atcagaaatc tctgacgccc gaaaagccct tcttcgtcta tttcgctccc ggcgcgacgc
     3601 acgcacccca ccatgtgccg aaggaatgga tcgcaaaata caaaggcaaa ttcgaccagg
     3661 gttgggacaa gctgcgcgaa gaaacgctgg cgcgccagat taaactgggc gtagtgcctg
     3721 ccggcaccaa actcgcggcc aagcctgcag ccatcacaga ctgggacaag ttaagcgcag
     3781 atgaaaaaaa actttttgcg cgccagatgg aagttttcgc ggccttcggc gaatacgccg
     3841 ataccgaaat aggacggctg gtcgatgcca tcggtagcat ggaccaactc gacaacacgc
     3901 tgatcttcta catcgtgggt gacaacggcg ccagcgccga aggcggcatg catggcctgg
     3961 tcaacgaata tacctacttc aaccaggtgc ccgaatcggt tgtcgatatc ctgaaacact
     4021 acgacgaact tggcggtccc aagacttttc cccactacgc agccggctgg gcggttgcag
     4081 gcgatactcc gtttgagtgg acgaaacagg ttgcctccaa ctacggcggt acccgtaacg
     4141 gaatggtggt ccactggccg aaggtcatac aagcgaaggg cgaagtgcga tcgcagtggc
     4201 atcatgtgat tgatatcgcg ccgacggttc ttgaggctgc cagcttgccg gagcctaagt
     4261 ccgtcaatgg aaccgttcag gagccgatcg aaggcgtgag catgctgtac tcgtttgcgg
     4321 atgccaaagc gaaagaccgg cacacgacgc agtacttcga aattttcggc aaccgggcga
     4381 tctacaaaga tgggtggctg gccggaacgg tgcaccgcgc agcatgggaa tacaaggtcc
     4441 gcgcgccgct cgaaaatgac atatgggagt tgtacaacac ccgcgaagac ttcagtcttg
     4501 cgaatgatat cgccgcgcag aatcccgaca agctcaaaga aatgcaggag ctttttttaa
     4561 tcgaagccgt caagtactcg gtgctgccgc tcgatgatcg cctgactgag cgctttatcg
     4621 ccggcatggt cggtcggccc gacctcatgg ccggccgtac ctcgctcacg gtccacgagg
     4681 gcatgatcgg catgtcggag aacgtattca tcaacatgaa gaaccgttcg cacacgatta
     4741 cggccgaagt gcaagttccg aagcaaggtg cgagcggcgc gatcatggcg caggcaggcc
     4801 ggttcggcgg ttggagcctt tacatgcagg acggcaagcc gatgtacacc tacaactggc
     4861 ttggattgca gcggtttacc gtggcttcca atacaccgtt gtctggaggc aaggcaacca
     4921 ttcgatttga attcgcttat gacggcggcg gcatgggcaa aggtggcacc ggtacgctct
     4981 tcgtcaatgg caagcaagtc gcgacgggac ggattgaacg cacgcagtgt tgcgtgtttt
     5041 cagcggacga aggtactgac gtgggcgcag acgagggaac gccggttacc gaagcgtaca
     5101 aggttccatt caagttcacg ggcacgatcg acaaggtgac cattgaactt aaagagatga
     5161 agctcgcgga taagggcgac gcggaacagg ctcacaagga agcgcgtctg aggaagggcg
     5221 tgtccgactg aagcgaagcg aaaaacgggg gaagcccttt ctgtgttccc ccgctttttt
     5281 tcccggttca gtttataaag tccacttcca ggttttgttt ccgattgtca caagtcttcg
     5341 tcatctcggt gttgctattt gctggatatc tgcgtccgaa gtcatgggac cgagttcact
     5401 cggctcaaga gcatttcctt gacccgttgc ggaccgatta cacttccaag ctcagacgca
     5461 agtgagtaca gaaccaatcc accgagagtc cacagcgcca tgacgctgac taaaggccac
     5521 gcgacttcgc tgaagaccgc ctggatcgca tcccgaacat tgccatgctt cagcaatagc
     5581 ggaacgagct cttcgataaa tttgaaaatg atcactactg cgccataaag cagcgttttc
     5641 cacagcactc tggtgaacag cagggacggc gacacccgga ccagtggaaa cgcctcgata
     5701 agcaggatgg ctttggccac aatgagcgcg cccagcgtcg cacttgtcgc acgaagggcg
     5761 gtaaagctga agtcattcaa cgatacggcc ctggtgagtg cgatcatgtg aaacaaaaac
     5821 aagaacagaa tagtcgcagg caggatttcc cggaactctg tcgcgataat cttgccgatt
     5881 tttcgcactt taattgctcc tcatcgccgc actgccggca atcactgtgg gcaacgcggc
     5941 actctcggga ttttgtttca tcaaccgcgc gcgcacaaaa tcaatatggg cacgcaaagt
     6001 gtaaagaaag tgtgaataca tgaatggcac ctgcagcttg ttggcgcggg cttccagccg
     6061 ccctacccgc tcgagcagct cctttaccgc cttggcatcg gacgtacgtg cggccaattc
     6121 cgcttcgagg aattttagtt ccccataaag aatgaatact cgtcgccgca cgatgtaacc
     6181 ataagtcgct gggatagatc gtatcagtgg gaatgcgacc ccaatga
//
LOCUS       DATMZQ010000034         8007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1752132, whole genome shotgun sequence.
ACCESSION   DATMZQ010000034 DATMZQ010000000
VERSION     DATMZQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752132"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VN496_01210"
     CDS             complement(<1..858)
                     /locus_tag="VN496_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXF15587.1"
                     /translation="MKKRTDALRCSVSVAMVCLVSACATMNQNGTTYNSAKKEMGDAA
                     KKAQAAKESENVDKALLPPLSVEMPKVDGRPIEARFDLAVNNAPASQVFMAIVSGTRY
                     SMVVHPAVKDQISVNLRDVTVFEALDTIREVYGYEYKVQGNRILIEPIGMQTRVYQVN
                     YLMSQRKGKTEMRVTSGAISDASNPANAGAVAQPAVVSGTGVTTTGMESSRISTSSNN
                     DFWSEISNALKAIIGTEGGRNVVMSPQAGVIVVRALPAELRSVSEFLKTMQVVVERQV
                     MLEAKIIEVQ"
     gene            complement(842..1132)
                     /locus_tag="VN496_01215"
     CDS             complement(842..1132)
                     /locus_tag="VN496_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15588.1"
                     /translation="MQSVLISPERRYAIINGEVVALGGSIAGAELISVAAERVTLRTR
                     DGLKTVHLFPDVTRLNDSEDVGATRHKPYSELPNDTKVQGSGNSGAKNEEKN"
     gene            complement(1276..1947)
                     /gene="gspM"
                     /locus_tag="VN496_01220"
     CDS             complement(1276..1947)
                     /gene="gspM"
                     /locus_tag="VN496_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HXF15589.1"
                     /translation="MTEFWAESQRKFDTFKPRERVMIFLGGVAIVAGLAFVLAIDGAL
                     AKHKIVAANVEKYRADLAQLEKRNAELSQLLAQDPDVVARKQIDELKQRLSSYDAELH
                     GVQQGLVPPKQMAKLLEDMLNRESQVRLVKLRTLPIAALVEPTTNAGNTVSKTTKNLV
                     YKHGMELTVEGGYLDLLEYQSRLEKLPWRMFFARTRVNAVDYPKVFMTLTLYTLSLEE
                     AWLVV"
     gene            complement(1944..2591)
                     /locus_tag="VN496_01225"
     CDS             complement(1944..2591)
                     /locus_tag="VN496_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXF15590.1"
                     /translation="MPQQINLYNPAFKARRSQLSLKAACTGWAAIAILLVAAASFQKL
                     NLHSLEQQERELSKKVSGAQAEAQRLAGQVGSRRHDPRIAEEVSHLEQEVQGRQKVVD
                     VLRAGDLGNTRGFSDHLKAFARQSFDGVWLTGLSIATSGRDVSVEGRALHASYVPGYL
                     KRLNGESAMQGHPFSELIIEEPSLDAAAKPPARPNYVEFKLTTNSERSATKAGEQ"
     gene            complement(2593..3546)
                     /locus_tag="VN496_01230"
     CDS             complement(2593..3546)
                     /locus_tag="VN496_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HXF15591.1"
                     /translation="MPSFFAKERTAELAVVNLYSDRVEVGHVRRNGPERPVVALCRHL
                     PCGEDPSEALQKARRELNLDRFRCSTVLPARQYQVQLVEAPSAVPNTEMKSAVRWRLK
                     DALDYPVELATVDMVTIPADRSGTGGGRSMYAVSARSQDIEACMNLFASVKLKLSVID
                     IAEMAQRNLATLFEIDDRAIAMLSFSSEGGLLTFSAQGELYLSRRIEISLDQLTDADG
                     EMRTKLYARIALELQRSLDHFDRQFSTIPLNRLLLAPLPDELNLPDYLAENLSAPVES
                     ANLGDVLDFHEAPELREQAEQLLRWQTLGAALRFESASQMN"
     gene            complement(3617..7663)
                     /locus_tag="VN496_01235"
     CDS             complement(3617..7663)
                     /locus_tag="VN496_01235"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HXF15592.1"
                     /translation="MTGNLTAVAGTGSGDHRQILLSNNAVLNVNGNITLNGSGNDNRF
                     ALLQVSGATATIGGNLVIDGLGGNDRARVTLANTSSLTVNGDITLNTGGLLDNGNTIN
                     SVIFLRGDFGHANADNDYVSTGGIFQVDGAGPQIFSGGAGITTTFYRLVINKASGDLT
                     LGHNIVVAGGAVSSLLVLTQGRIVTAANSVGVGAAGATSTTINGASTASYIVGNLRRY
                     IPTGTQSGVMFPVGGSTAGKYAQVQIDYPNVTLAGYFQVAASLADTDHPNIASSALDP
                     AASVNRYWTLTNSGGITFTGATTANVTFNYPTSDVDAGADTSAFIVGDFSGGAWTYPV
                     TGTLTSNSTQMTGLTAAAIAGDFAIAELIGPYSYWRMNENSWNGTANEVGDFGSSSNP
                     GTAASLSATKPTTSNTSPAIAGTPGTCRYGVFNRTNKDYIALPTTYPNLATSSFTITA
                     WIRTTDRTQPGQRILIDDEGTTGGWGFSLGDGLAGALRLYSRGSATNTLDTAALINNN
                     TWYFVAVTINISGTTTIATIYVYTTAGALAGSSTATWAAFTWGNDTGRPSIGGETNAS
                     SEGTNSFGFAGNIDEVRVYRAALTQSMVNNVRQLTTPCIVVDHYALAGSATAVTCDAS
                     TVQVIAHDVAHGLVAPASGTILTLSTSTGTGVWQAGLLSGTGAWAPSGSNNGVATYVW
                     PGGESTFSVMLRQNTAAALGINVSDSNGKVEAVTEDLSIGFVDSGFRVTTNGTSTATI
                     PTQIAGKNSNTGFGAQTLYLQAIRTDTNTGSCVGLIQSQTVTIEMAAARINPTGSASQ
                     VSVLNSSNGLVPLGTGAGVAGSYTNVTLAFDAQSKAPLVVNYANAGSVTLFARYQLPT
                     PPASTFVSGSSNTFVVRPFGFRISGPPSGRSGPGSTVYAKAGQTWPDAVTVTAVAWES
                     ADDLNNDGVPDSDAALAGNAITSNFGLESTPATVTISHTLAEPSGGNSGTLSAPLSVF
                     AGGSASASASWSEAGLINLFTLSSNYLGGGQNVSNSASGYTGVGRFTPFDFAVARNSP
                     AFNAACSSNFTYVGQLFNYSTAPALTVTARNSSGSTTQNYAGTFMKITSASLTPNTVA
                     ARYAAFDALGGGTTPAVNTANVPGAPGVGSDPTIGTFTNGVGTLTFSAGTGLKFTRST
                     TTPNLPFNADIALSFNLIDTDNVAVARIDGVAAVNPVSFGAATAGNGIAFSPAAANQM
                     RFGRLKLSNAFGSELLDLPIPIETQYYNSSGVYVTNTADNCTAIALNSTFLSSGTATV
                     GGSFVSGKGSLKINKPLSKVTIDLCVDLDISAASQNDSTCIATTPSNQNWLQWKWTGA
                     TFDKDPKARASFGVFKNADEFIYLRENF"
     gene            7725..>8007
                     /locus_tag="VN496_01240"
     CDS             7725..>8007
                     /locus_tag="VN496_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15593.1"
                     /translation="MDRTVGEIFTVTVTPSQIVTSSAAVGKAAPVPAAQLVADQVRAL
                     QLPELRAKRAAACAFDVSKHAVISNTDATVVIRTRRAANQILPCSCCIVA"
BASE COUNT         1646 a         2311 c         2199 g         1851 t
ORIGIN      
        1 ctgaacctcg ataattttag cctccagcat gacctggcgc tcgactacaa cttgcatagt
       61 cttcaggaac tcggaaacac tacgcagttc tgccggcaaa gcccgtacca cgatcactcc
      121 ggcttgcggg ctcattacca cattgcgccc gccttccgtg ccgatgatgg cctttagcgc
      181 attgctgatt tcgctccaga agtcattgtt cgaactggta ctgatgcgac tgctttccat
      241 tccagtcgtc gtcacgccgg tgccactaac gaccgctggc tgcgcaacgg ctccggcgtt
      301 cgcagggttg gacgcatcgg aaatggcgcc agaggttacc ctcatctcgg tcttgccttt
      361 gcgctggctc atcaagtaat tcacttgata cacgcgcgtt tgcatgccga tcggctctat
      421 caatatccgg ttaccctgga ctttgtactc gtagccatac acttcccgaa tcgtgtcgag
      481 tgcttcgaat accgtcacat ctcgcagatt gaccgagatt tgatccttaa ccgcaggatg
      541 taccaccatg ctgtatcggg tgccggatac gatggccatg aaaacctggc ttgccggcgc
      601 gttatttact gccaaatcaa agcgcgcttc aattggccta ccatcgacct tcggcatctc
      661 caccgaaagt ggcggaagca aagccttgtc cacgttttcc gattctttag ccgcttgagc
      721 ctttttggcg gcatctccca tttctttttt tgcgctgttg tacgtagtac cgttttggtt
      781 catcgtcgca cacgcgctca ccagacacac catggccacg gagacagaac accgaagggc
      841 gtcagttctt ttcttcattc ttcgctccgg aatttccgga cccttgaacc ttcgtatcgt
      901 tcggcaattc cgaataaggc ttgtgtcgcg tggcgccgac gtcttcactg tcattcagcc
      961 gggtcacatc cggaaacaaa tggacagttt tcaacccatc gcgggttcgc agagtgaccc
     1021 gttctgccgc gacggaaatc agttctgcac ccgcaattga accgcccaaa gctacgactt
     1081 cgccattgat gatcgcatag cgccgttcgg gcgaaataag caccgattgc aaattccacc
     1141 ccgatggatt caattttccg gggaggcttt tgccgcttcc aaacgacggc cgcgtcgggt
     1201 cccgcagccc ctgtgcgacg cccgcagacg cgctcccaag gcagaggcct gctatcaaaa
     1261 ttgaggcgta gcgattcaaa ccaccaacca cgcttcctcc agactcagcg tatatagcgt
     1321 caatgtcata aatacttttg gataatcgac cgcgttcacc cgcgtgcgcg cgaagaacat
     1381 gcgccaggga agtttttcca gccgcgattg atattccagc aggtcgaggt acccaccctc
     1441 taccgtcaac tccataccat gcttgtagac aagatttttc gttgtcttgg agaccgtatt
     1501 gcccgcgttc gtggtcggtt caaccaacgc ggcaatcggc aaggtacgta atttgaccaa
     1561 tctcacctgg gactcccggt tcagcatgtc ttccaggagt ttcgccatct gtttcggtgg
     1621 taccaaacct tgctgaacgc cgtgcagttc cgcgtcatag ctactcaacc gttgcttcag
     1681 ctcatcgatc tgcttgcgcg cgaccacatc gggatcttgt gctaacagct gcgaaagttc
     1741 ggcatttcgc ttttccagtt gcgccagatc cgcgcgatac ttttcaacgt ttgctgcaac
     1801 aatcttatgc ttggcaagcg ctccgtcgat tgctagaaca aaggccagtc cggcgacaat
     1861 tgctacaccg cccagaaaga tcatgacgcg ttcgcgcggc ttgaaggtat caaatttgcg
     1921 ttgtgactcc gcccaaaatt cggtcattgc tcgcccgcct tggtggcgga ccgctcagaa
     1981 ttggtggtca gcttgaattc cacgtaattc gggcgggccg ggggcttcgc ggccgcatcc
     2041 aacgagggct cttcgatgat caactccgaa aagggatgtc cttgcattgc gctttccccg
     2101 ttcagccgtt ttagatagcc cggaacataa gaagcgtgca gcgcccttcc ttcgactgat
     2161 acgtctcgtc cggacgttgc aatgcttaac ccggtaagcc acacaccatc aaacgattga
     2221 cgcgcgaaag ctttgagatg atcagagaat ccgcgggtat tgccgaggtc ccccgcgcgc
     2281 aggacgtcga cgaccttctg gcgaccttgt acttcctgct ccagatgact tacttcttcc
     2341 gcgatccgcg gatcatgtcg tctggagcca acctggccag ccagtctttg tgcttcggcc
     2401 tgcgctcctg aaaccttctt cgataattca cgctcctgct gctccaaaga atgcaaatta
     2461 agcttctgga aactggcggc cgcgacaagc aagattgcaa tcgcggccca acctgtgcaa
     2521 gcggctttca acgacaactg tgaacgtcgc gctttgaagg ccggattgta gagattgatt
     2581 tgctgtggca cgtcagttca tctggcttgc tgattcgaag cgtagcgctg ccccaagcgt
     2641 ttgccagcgc aataactgct ctgcctgctc gcgaagttca ggcgcctcat ggaaatcgag
     2701 aacatccccg agattcgccg attccaccgg tgcggataga ttttccgcga gataatccgg
     2761 aagattaagt tcgtcaggca agggcgcgag caataggcgg ttcaatggaa tagtgctgaa
     2821 ttgacgatcg aaatgatcca gtgagcgctg caactcgagc gctatgcgcg cgtaaagctt
     2881 cgttcgcatc tctccgtcgg cgtcggtaag ctgatccagg ctgatctcga tacggcgcga
     2941 aagataaagt tctccttgcg cgctgaatgt cagtaacccg ccttcactgc tgaaactcag
     3001 catggcaatt gcccgatcat cgatttcgaa tagcgtggcg agattgcgtt gcgccatttc
     3061 tgcgatatcg atgacgctca atttgagttt gaccgacgca aagagattca tgcatgcctc
     3121 gatatcctga ctgcgggcgg acaccgcata catcgaccgt cctccgccag ttcctgaccg
     3181 atccgcggga atcgttacca tgtcgacagt ggcgagttcg accggatagt ccagcgcatc
     3241 cttgagccgc cacctcactg ctgatttcat ttcggtattg ggcacggcgg atggcgcctc
     3301 taccaattga acttggtatt gccgagccgg aaggacggtg gaacagcgaa aacgatcgag
     3361 gtttaattcg cgccttgctt tctgaagggc ttcactggga tcttcaccac aaggaaggtg
     3421 tcggcacaac gccaccacag gacgctcggg accgttgcgt cgaacgtgtc cgacctccac
     3481 gcggtcgctg tagagattca ccaccgccag ttctgcggtg cgttccttgg caaaaaacga
     3541 tggcatttta tggttagtta attctatacg tgaaaatata tcaataacct attgtcgtca
     3601 atgtcctttt tatacactaa aagttctccc ttaaatagat aaactcgtcc gcgtttttaa
     3661 agacgccaaa gctggcgcgt gctttcggat ctttgtcgaa cgttgcaccc gtccacttcc
     3721 actgcagcca gttttggttt gacggggtgg tcgcaatgca agttgaatcg ttctggctgg
     3781 ccgcgctgat gtccaggtca acacataaat cgatagttac tttgctcagc ggcttgttaa
     3841 tcttcaggct gcctttgccg gacacaaagg aacctcctac cgtagccgtc cccgagctca
     3901 ggaaggtact gttgagcgca atcgccgtgc aattgtcggc cgtgttcgtg acatatacac
     3961 cgctgctgtt gtagtactga gtttcaatcg ggatgggcag gtccagtaac tccgaaccaa
     4021 atgcattgct aagcttcagt cgaccaaaac gcatttgatt cgctgcagct ggagagaaag
     4081 cgatgccatt gcccgccgtg gccgcgccga agctgacagg attgacagcg gcgacaccgt
     4141 caattcttgc gacagcgacg ttgtcggtat cgatcaggtt aaaggaaagc gcgatatcgg
     4201 cattgaatgg caaattcggc gtagtggtac tccgcgtaaa cttaaggccg gtacccgccg
     4261 aaaacgtcag cgtgccgaca ccattggtaa acgtgccgat ggtgggatca cttcctactc
     4321 cgggcgcacc tggtacgttc gcagtgttga ccgctggcgt cgtgcctcct cccaatgcgt
     4381 cgaaagctgc atagcgcgct gccactgtat tcggcgtgag actcgcgctg gtgatcttca
     4441 tgaatgtgcc cgcgtagttc tgggttgtgg atcccgaact attgcgcgcg gttaccgtaa
     4501 gggcaggggc ggtcgaataa ttgaacaact gtccgacata cgtgaaattg ctggagcaag
     4561 ctgcattgaa cgctggcgaa ttgcgtgcga ccgcgaaatc aaatggggta aatcgcccga
     4621 ctcccgtata tccggatgcg gagttactga cattttgtcc gccgccgaga taattgctgg
     4681 aaagggtgaa aagattgatc aggccggctt cgctccagct cgccgaggcg ctcgccgaac
     4741 cacctgcaaa taccgacagg ggagcggaca acgttccgct gttgccgccg gacggttcag
     4801 ccagggtatg acttatggtt acagttgcag gcgtagattc caggccgaag tttgaagtga
     4861 ttgcattgcc tgccaaagcg gcatcgctgt ccggaacgcc atcgttattc agatcgtcgg
     4921 cactttccca ggcaaccgca gttaccgtga ccgcatccgg ccaggtttgc ccggcttttg
     4981 catacaccgt actgccgggc ccactgcgcc cggatggcgg accggatatt cgaaatccga
     5041 atggcctcac gacaaaagta ttcgagcttc cagaaacgaa agtgctggct ggaggcgtgg
     5101 gcaactggta gcgcgcgaac agcgtgacgc tgcccgcgtt cgcgtagttt acgaccaggg
     5161 gtgccttcga ttgcgcgtcg aatgccagcg tcacgttggt gtagctcccg gcaacgccgg
     5221 cgcccgtccc caatggaacg aggccgttgc tgctgttgag tacgctcacc tggctggcgc
     5281 tgccggtcgg attgatacga gcggctgcca tttcaatcgt cacggtttgg ctctggatta
     5341 agcccacaca gctgccggta ttggtatcgg tacggattgc ctgcaggtat agggtttgtg
     5401 caccgaagcc ggtgttggaa tttttgcctg cgatctgggt tggaatagtt gcagtcgaag
     5461 taccgttggt ggttacgcga aaaccagaat ccacgaagcc aatgcttagg tcctcggtga
     5521 ccgcctccac cttgccgttg ctgtcgctca cattgatgcc aagcgctgca gcggtgttct
     5581 ggcgcagcat tacgctgaac gtcgattcgc cgccgggcca gacataggtt gccacgccgt
     5641 tattggaacc ggatggcgcc cacgcacccg tgcccgacag caggccggcc tgccagactc
     5701 cagtgccagt cgacgtagac agtgtaagaa tggttcccga tgcaggtgca acgagaccat
     5761 gcgcaacatc gtgtgcaatg acttgaaccg ttgatgcatc gcaggtcacg gcggtcgcgc
     5821 taccggccag tgcatagtga tcgaccacga tgcacggagt agtgagctgc cgtacattgt
     5881 tgaccatgct ctgcgtcagc gcggcacgat agacccggac ttcgtcgatg ttgccggcaa
     5941 agccgaaact gttcgtccct tcggacgaag catttgtttc gccgcctatg gacgggcgac
     6001 cggtatcgtt gccccaggtg aaggccgccc atgtggctgt ggaggacccg gcaagtgctc
     6061 ctgccgtggt gtaaacataa atcgtcgcta tcgttgtggt gccgctgatg ttgatcgtca
     6121 cagcgacgaa gtaccaggtg ttgttgttga tcagcgcagc ggtgtccagc gtattcgtgg
     6181 ccgaaccgcg cgagtacaaa cgcaatgccc cggccaaacc gtctcccagg ctgaatcccc
     6241 agcctccggt cgtcccttca tcgtcgatca gaatccgctg gccgggctgt gttctgtccg
     6301 tggtccggat ccaggccgtg atcgtaaagc tgctggtggc gaggttcgga taagtcgtcg
     6361 gcaacgcaat gtaatccttg ttggtgcggt tgaatacgcc atagcggcag gtgccgggcg
     6421 tgccggcaat cgccggcgac gtgttgctgg tcgtgggttt ggtcgcggac aggctggccg
     6481 ccgtccccgg attgctgctg ctgccgaagt cacccacttc attcgccgtg ccgttccatg
     6541 aattttcatt catgcgccaa tatgaatagg gaccgataag ttccgcgatg gcgaaatcgc
     6601 ctgcaatagc tgccgcagtc aggccggtca tttgcgtgct gttcgaagtc agcgtgcctg
     6661 tcaccggata tgtccatgcg ccgcccgaaa aatcgccgac gatgaaggcc gaagtgtccg
     6721 cgcccgcgtc aacgtcgctg gtcggatagt tgaacgtgac gtttgcggtg gtggcaccgg
     6781 taaatgtgat gccgccactg ttggtcagcg tccagtaacg gttcacactc gcagcaggat
     6841 cgagtgctga cgatgcaata tttggatggt cggtatcggc aagcgaagct gcgacctgga
     6901 aatagcccgc cagagtcacg tttggatagt cgatttgcac ttgcgcatac ttgcccgcgg
     6961 tgctgccgcc gaccgggaac atgacgccgg attgcgtacc ggtagggata tatctgcgca
     7021 aattgcccac gatatagctt gccgtactcg ccccgttgat agtcgtggat gttgcgccgg
     7081 cggccccaac accaaccgag ttcgccgctg tcacgatgcg cccctgtgtc agcaccagca
     7141 gagacgacac tgcaccaccc gccacgacga tgttgtggcc gagcgtcagg tcacccgatg
     7201 ccttgttaat gaccagtctg tagaaagtcg tcgtaatgcc tgcgccgcca ctgaaaattt
     7261 gcggcccagc gccgtccacc tggaagatgc cacccgtgct gacatagtcg ttgtctgcgt
     7321 tggcgtgacc gaaatcccca cgcaggaaaa tcacgctgtt tatggtattg ccgttatcga
     7381 gtaacccacc ggtgttcagc gtgatatcgc cgttcaccgt aaggctcgat gtattggcaa
     7441 gagtcacccg tgcccgatcg tttccaccaa ggccgtctat caccaggttt cccccgatcg
     7501 tggcggttgc gccgcttacc tgcagcaacg caaagcggtt gtcgttgcca ctgccgttca
     7561 gggttatgtt gccatttacg ttaaggaccg cgttattgga gagtaggatt tgtctgtggt
     7621 cgccgctgcc ggtacccgcg acagccgtta gatttcctgt cactgtcaac gtttgcgcgg
     7681 acgtgatctg caaggtcgtc agcgtccccc ccgtgggaat gctgatggac agaaccgttg
     7741 gagagatatt caccgtcacc gtaacgccgt cgcaaatcgt cacgtcgtcg gctgcggtcg
     7801 gcaaagccgc tcccgtcccg gccgcacagc tggtagccga ccaggtgagg gcgctccagt
     7861 taccggagct gcgcgcaaag cgcgcggccg cctgcgcatt cgatgtcagc aagcacgctg
     7921 tgattagcaa caccgacgca accgtggtta tccgcacgcg tcgcgcagca aaccaaatct
     7981 tgccgtgttc gtgttgcatc gtcgcct
//
LOCUS       DATMZQ010000035         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2084847, whole genome shotgun sequence.
ACCESSION   DATMZQ010000035 DATMZQ010000000
VERSION     DATMZQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084847"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VN496_01245"
     CDS             complement(<1..904)
                     /locus_tag="VN496_01245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXF15594.1"
                     /translation="MTAYRDELKAVAFPALLLLIAGIMAMTSHKLPASLGSFAVFGPY
                     TVLPIGTAIALWFNRGRAFIALISLLVAFAAFRIALGFGVGDFPVHAVLTAAAIFVPA
                     NILIVLRLPERGIVHFRNYRWALLGVVEIVLTVWIATAGRTTFSGTSWVEGLNHWLLK
                     AAPTPLLGRLFMAAALVMALVRAWPKFSPLDVGMVGAVIAFFLACEWPNHASVYGIFI
                     FAAAAILLLAVLQESHRMAFHDELTGLPSRRALQEKLVALGPAYTIAMVDVDHFKKFN
                     DTHGHEIGDQVLKLVGARLAAIDGG"
     gene            complement(951..1247)
                     /locus_tag="VN496_01250"
     CDS             complement(951..1247)
                     /locus_tag="VN496_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXF15595.1"
                     /translation="MRKATKPVGGHSARRVRNGRSGNQITETGASPRGKIRLTMYLDM
                     AVVEYFRSRAGGRGYQTLINEALKQAIEQESLEDVLRRVIREEARPYQTGKGKS"
     gene            1441..>2776
                     /locus_tag="VN496_01255"
     CDS             1441..>2776
                     /locus_tag="VN496_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXF15596.1"
                     /translation="MSPDPLILARAQFGLNIGFHILFPSITIALAWILFYLRVRIEFF
                     GDAWCNMAYRLWVKVFALSFALGVVSGITMSFQFGTNWPGFMNRAGNIAGPLLGYEVL
                     TAFFLEATFLGVMLFGRDRVPAWMHTLSAFLVAIGTSASAFWILALNSWMQTPQGYEV
                     IDGQLHATSWMAVIFNASFPYRLAHMMIASGLTAAFLVAGLSAYQLLRHRTFEPAARM
                     LRLGVLIAALLAPVQIVVGDFHGLNTLAHQPAKIAAIEAIWRTERGAPLLLFAMPNER
                     TRSNDYALAVPKGASLILTHELEGEVKGLDQFGDKHPPVAAVFWTFRVMVGMGMLMLV
                     SSWLGMIYLMRRLGFPRWLLRVYAAMAFSGWIAVLAGWMTTEIGRQPYLVYGVLLTAE
                     TASAVPAENIAITLIGYTLVYGLLLVSYMVVITQLAAKEAEGKGREEPLPGSL"
BASE COUNT          531 a          801 c          832 g          612 t
ORIGIN      
        1 cgccgccgtc gattgccgcg agacgagctc cgaccagttt cagcacctga tcgccgatct
       61 catgaccatg cgtgtcgttg aacttcttga aatgatcgac gtcgaccatc gcgatagtgt
      121 acgcagggcc gagcgcgacg agtttttcct gcagagcccg gcggctgggc aagccggtca
      181 attcgtcgtg gaacgccatg cggtgcgact cctgcaggac cgccagcagc aatatggcgg
      241 cggcggcaaa gatgaaaatc ccgtacaccg acgcatggtt tggccattcg caggccagga
      301 agaatgcaat gactgcccca accatgccga catcaagagg cgaaaatttc ggccaggccc
      361 tgacgagcgc catgactaat gccgctgcca tgaacagccg tccgagcaac ggcgtgggcg
      421 ccgctttcag cagccagtgg ttcaggccct cgacccagga tgtgcccgaa aaggtcgtgc
      481 gtccggcggt tgcgatccag actgtcagca ctatttcgac tactccgagc agcgcccacc
      541 ggtaattgcg gaagtgcacg atgccgcgtt ccggtaaccg cagcacgatc aggatattgg
      601 ctggcacgaa aatggctgct gctgttagca cggcatgcac cggaaaatcg cccacaccga
      661 aaccgagggc aatgcgaaag gcggcaaatg ccaccagtag agaaatcagc gcgatgaatg
      721 cgcggccgcg gttgaaccac agcgcgatcg cagtcccgat gggaagcacc gtgtacggcc
      781 cgaaaacggc gaaactgccc agcgatgcag gcaatttgtg gcttgtcatg gccatgatgc
      841 ccgctatcaa aagcagcagg gcagggaaag caactgcctt gagttcgtcg cgataagcag
      901 tcatgcagcg tgcatcgggc ggtcaagtgt tgcagcctcg aatccgatga tcaagacttg
      961 cctttccccg tctggtaggg acgcgcttct tcccggatca cacggcgcag cacgtcttcc
     1021 aggctttcct gttcgattgc ctgcttgagc gcttcattga tcagtgtctg gtaaccccgg
     1081 ccacctgcac gtgaacggaa atattcgacg actgccatat cgagatacat ggtgagccgg
     1141 atttttccgc gcggcgacgc tcctgtttcg gttatttgat tgccggagcg gccattccgg
     1201 acgcggcgcg cggaatgccc cccaaccggc ttggttgctt tacgcatgaa tttcagtgtg
     1261 catacaacta tacatatagt caagccgtgc cgccgaaccc gttgcgcaac gtgcgcgcag
     1321 tcgataatcc gagacatttg gtggcgaaac actgtccgct gtcgatctcg ttggcagtcg
     1381 ctcgagggcg ggtatgcggt atacgcgcta aactgacgcg cgtcgttatc gaggatcatc
     1441 atgtcgccgg atccgctcat ccttgcgcgc gcgcagttcg gcctgaacat cggtttccac
     1501 attctttttc ccagcatcac cattgcgctg gcctggatac tgttttacct ccgggtgcgc
     1561 atcgaattct ttggagatgc ctggtgcaat atggcctacc gtctctgggt gaaagtattc
     1621 gccctgtcgt tcgcgctggg cgttgtttcc ggcatcacca tgagctttca gtttggcacc
     1681 aactggccgg gcttcatgaa cagggcgggc aacatagccg gacctttgct gggctatgag
     1741 gtgctcacgg cgtttttcct cgaagcgacc ttcctgggcg tcatgctgtt cggccgcgac
     1801 agggtgccgg cctggatgca caccttgtcg gcgttcctgg tggcgatcgg tacgtcggct
     1861 tcggcgttct ggattctcgc tctcaattcc tggatgcaga ctccgcaggg atacgaggtc
     1921 atcgacggtc agctgcatgc gacaagctgg atggccgtta tattcaatgc gagttttccg
     1981 tatcgcctgg cgcacatgat gattgcctcg ggtctgacgg ccgcctttct ggtggccggg
     2041 ctttccgcct accagctgtt gcgtcaccgc acgttcgaac cggccgcgcg catgttgcgc
     2101 ctcggcgtgt taattgccgc gttgcttgcg ccagtacaga tcgtggtcgg agattttcac
     2161 ggtcttaata cgctcgcaca ccagccggcg aaaatcgccg cgatcgaggc gatctggcgg
     2221 accgagcgcg gagcgccgct actgctgttt gccatgccaa acgaacgaac ccgcagcaat
     2281 gactatgcat tggcggtgcc gaaaggcgca agcctgattc tgacgcatga attggaggga
     2341 gaggtaaagg ggctcgatca attcggcgac aaacatcctc cggtcgcggc cgttttctgg
     2401 accttccgcg tgatggtggg catgggcatg ctcatgctgg tcagttcatg gcttggcatg
     2461 atctatctga tgcggcgtct cggttttccc cgttggctgt tgcgggtgta tgcggccatg
     2521 gccttctcgg gctggatcgc cgtgctggcg ggttggatga ctactgaaat tggacgtcag
     2581 ccttatcttg tctacggagt actcttgact gcggaaactg cctcggcggt tcccgctgaa
     2641 aatattgcga tcacgctcat tggctacacg ctcgtttacg gcctgctgct ggtttcctat
     2701 atggtcgtta tcacgcagtt ggcggccaag gaggcggaag gcaagggccg cgaggaacca
     2761 cttccgggca gcttgg
//
LOCUS       DATMZQ010000036         4613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2789629, whole genome shotgun sequence.
ACCESSION   DATMZQ010000036 DATMZQ010000000
VERSION     DATMZQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789629"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VN496_01260"
     CDS             complement(<1..273)
                     /locus_tag="VN496_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine phosphorylase"
                     /protein_id="HXF15597.1"
                     /translation="MLGIIGGTGLTQLSNLEVTRRMIVRTPYGDASGALTFGKLNGRN
                     VVFLARHGYGHTIPPHEVNYRANIWALHSEGVARVVSVASVGGIRSD"
     gene            complement(285..830)
                     /locus_tag="VN496_01265"
     CDS             complement(285..830)
                     /locus_tag="VN496_01265"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HXF15598.1"
                     /translation="MPNKEQAWRLLREADLLFDEDEVGRTVRRLALDITAKLAESYPL
                     VMPVMGGAVVFTGHLLPLLHFPLEFDYLHVTRYDGSTQGGQVQWKTKPHVDFRGRTVL
                     VLDDILDEGNTLAAIRDHLLGEGAAAFYSAVFAEKDLGRKKPIKPDFLGLTVPDRYVF
                     GFGMDAYECWRNLPAVYALKS"
     gene            complement(820..1719)
                     /gene="galU"
                     /locus_tag="VN496_01270"
     CDS             complement(820..1719)
                     /gene="galU"
                     /locus_tag="VN496_01270"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXF15599.1"
                     /translation="MNKITKAVFPVAGMGTRFLPVTKASPKEMLPVVDKPLIQYAVEE
                     AVAAGITQMVFITGRNKRAIEDHFDKAYELETELAARNKHEMLALLQGILPKHVSCIY
                     VRQTEALGLGHAILCARPVVGSDPFAVILADDLIDGNPPVMKQMVDVYTSERCSILAV
                     QTVARSETASYGIVRTEPGERGMHRITGIVEKPKPDVAPSTLAVVGRYILNPEIFGHL
                     ERVKPGAGGEIQLTDGIAALLAEQRALAYEFQGVRYDCGSKLGYLEANVLFAMRHPEI
                     SGEFCEFVDSLRAREKRTKTGAK"
     gene            complement(1781..3808)
                     /gene="ligA"
                     /locus_tag="VN496_01275"
     CDS             complement(1781..3808)
                     /gene="ligA"
                     /locus_tag="VN496_01275"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXF15600.1"
                     /translation="MAVPSPAAERAARLRRDIDAANYNYYVLDTPSMPDAEYDRLFRE
                     LQALELQYPELATPDSPTQRVGGEVRSDLPKVRHTVPMLSVRTETDSHGGALTFDTRI
                     RRELKLGPDDPPIEYNAELKFDGIAISLRYESGVLVRAATRGDGEIGEDVTSNVKTIR
                     SIPLRLRADHPPAVLEVRGEIFMRRAEFEKLNERQRAAGEKVFVNPRNAAAGFVRQLD
                     SRITASRPLFCYAYGLGEVDGWALPGTQSAILDALYAFGVPVSEQRTVAHGADELLAF
                     HTQIAQTRDRLPFDIDGVIYKVNSIALQRQLGFVSREPRWAVAHKYPAQEEQTQVLGV
                     EVQVGRTGKLTPVGKLQPVFVGGVTVSNVTLHNEDYIKELDLRIGDTVIVRRAGDVIP
                     QVVAVRSELRPSGARRFSMPARCPVCGSEVVREEGETDHRCTAGLFCPAQRKQALRHF
                     AGRRAMDIEGLGEKLIDQLVDADRVKTPADLYRLDLPTLSNFERMAEKSATNVLAAID
                     KSRATTLPRFIFALGIRNVGETTARDLARHFGTLDALMQADETALQEAPDIGPVVAQS
                     IVHFFGERHNRDVIAQLRAAGVHWPEEQVARSVALPLAAKTFVLTGTLPNLTRDEAKQ
                     RIEAQGGKVTGSVSKKTDYVVAGADPGSKLDKARELGVAVLDERGLLELLE"
     gene            complement(3810..>4613)
                     /locus_tag="VN496_01280"
     CDS             complement(3810..>4613)
                     /locus_tag="VN496_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HXF15601.1"
                     /translation="RREIDYIAELRAGEFIGAHQIAKVRHRIPALGRRITLGGLNHES
                     KSWEPLRTDGRYTTLRLGLQLVDRSGPAKEEQLRTFADFVKTAASELSAIAELPEFAP
                     SLEQAAALDEFCADVDVLVGINVIANTGQVFHGTKIRGLAEASGLRLQSSGVFHCHDE
                     QGSTLFSLDNQQSEPFLIDRIRNITTPGVTFLLDVPRVANGLQVFDRMVAMSRSFAES
                     LDGILADDNRALLNDAGLDRIRAQLRAIYSAMEQRGIDAGSPLALRLFS"
BASE COUNT          899 a         1443 c         1312 g          959 t
ORIGIN      
        1 atcggatcga atgccgccaa cggacgctac ggagaccacc cgcgcaactc cctccgagtg
       61 cagtgcccag atgttggccc gatagttcac ttcgtgaggc ggaatcgtat ggccgtagcc
      121 gtggcgcgcc aggaatacca cgttgcggcc gttcaatttt ccgaacgtca gcgcgccgga
      181 agcatctcca tacggcgttc tgacaatcat tctacgagtc acttccaggt tggacagctg
      241 tgtaaggccg gtgccaccaa taattccaag caatgctttt ctcctcagga cttcagcgca
      301 tacacggcag gcaaattccg ccagcattca taggcatcca tgccgaaccc gaacacatat
      361 cgatcgggga cagtcaagcc caggaaatcc ggcttgatcg gttttttgcg cccgagatct
      421 ttttccgcga acaccgcgct gtaaaaagcc gccgcgcctt ccccgagcag atgatcgcga
      481 atcgccgcga gcgtgttgcc ttcatcgagg atgtcgtcca gcaccagcac cgtgcgcccg
      541 cgaaaatcca cgtgcggctt ggtcttccac tgcacctgtc cgccctgggt gctgccgtca
      601 tagcgcgtca cgtgcaggta atcgaactcg agcggaaagt gcagcagggg cagcaggtgc
      661 ccggtgaata ccacggcgcc ccccatgaca ggcatcacca atggatagga ttcggcgagc
      721 ttggcggtaa tgtcgagcgc caaccgtctt acggttcggc cgacttcatc ttcgtcgaac
      781 agtagatccg cctcgcgcag tagtcgccat gcctgttcct tatttggcac cggttttggt
      841 ccttttctcc cgcgccctca gcgaatcgac aaactcgcaa aattcacctg aaatctcggg
      901 atggcgcatc gcaaacagca cgttggcttc cagataaccc agcttcgagc cgcaatcata
      961 gcggacgccc tggaattcat aggccaatgc acgctgttcc gcgagcagcg ctgcaatgcc
     1021 atcggtcaat tgaatctccc ctccggctcc tggttttacc ctctcgagat gaccgaagat
     1081 ctcgggattc agtatgtagc ggccgacaac cgccagcgta gaaggcgcca catccggttt
     1141 tggtttctcg acgattccgg tgatgcgatg catgccccgc tcgccgggct ctgtcctgac
     1201 aatgccatag ctggctgtct cactgcgcgc aacggtttgc acggcgagaa tcgagcagcg
     1261 ctcgctggta tagacatcaa ccatctgctt catgacggga gggttaccat caatgaggtc
     1321 gtcggccaat atgaccgcga agggatcgct gccaaccacc ggtcgggcgc acaatatggc
     1381 gtgcccaagt ccgagcgcct ccgtctggcg cacgtagatg cagctgacat gctttggcag
     1441 aatgccttgc aacagcgcga gcatctcgtg cttgttacgg gcggccaact cggtctccaa
     1501 ctcgtaagcc ttgtcgaaat ggtcctcgat ggcgcgcttg ttgcggccgg ttatgaacac
     1561 catttgggtt atgccagctg cgaccgcctc ctctacggcg tactggatca ggggcttgtc
     1621 gactaccggg agcatctctt tcggactagc cttggtgacc gggagaaatc gggtccccat
     1681 gccggcaact ggaaataccg ctttggtgat cttgttcaag tgttgctcct gggaggccgg
     1741 acgcacaaat acccaattgc catgcgcatc cgatagtggg ttattcaagc aattctaaca
     1801 accctctttc gtccagcaca gcgacgccaa gttcccgcgc cttgtccagt ttgctgccgg
     1861 ggtcggcgcc tgccactacg taatcggttt tcttcgatac gctgcccgtg actttgcccc
     1921 cttgcgcctc gatgcgttgc ttcgcctcat cgcgcgtcag gttgggcagt gtccctgtta
     1981 gcacgaacgt cttcgccgcg agcggcagcg ccactgatct tgccacctgc tcttcgggcc
     2041 aatgaactcc ggccgcgcgc agctgggcaa tgacatctcg attgtgccgt tcaccaaaaa
     2101 aatgaacgat gctttgcgcg accaccggac cgatgtccgg agcttcttgc agtgccgttt
     2161 cgtccgcttg catcagcgca tccagcgtgc cgaagtgacg tgccaggtcc cgggcagtgg
     2221 tttccccgac attgcggatg ccgagcgcga aaatgaaacg cggcaatgta gttgctctgc
     2281 tcttgtcaat ggccgcgagc acattggtcg ccgacttttc ggccatgcgc tcgaaattgg
     2341 acaaggttgg cagatcaagc cggtacagat ccgcaggcgt tttgacccga tctgcgtcca
     2401 ccagctgatc gatcaatttt tcacccaacc cttcaatgtc catcgcgcgt cgaccggcga
     2461 agtgccgcag cgcctgtttg cgctgcgccg gacagaacag ccctgcggta cagcgatggt
     2521 ccgtctcgcc ttcttcccgc acgacttcgg aaccgcacac cgggcaccgg gccggcatcg
     2581 aaaaacgacg cgcgcccgag ggccgcaatt cgctgcgcac agccacaacc tgcgggatca
     2641 catcaccggc gcgccgtacg atcaccgtgt caccgatgcg aagatcgagt tccttgatgt
     2701 agtcctcgtt atgcagggtc acattgctga ccgtcacgcc accgacaaat accggctgca
     2761 gttttccgac cggcgtcagc ttcccggtgc ggccgacctg aacttcgacg cccagtacct
     2821 gcgtctgctc ctcttgtgcc gggtacttgt gcgcgactgc ccagcgtggt tcgcgcgata
     2881 cgaaaccaag ttgacgctgc agcgcgatgc tgttgacttt ataaatcacg ccgtcgatat
     2941 caaacggcag ccggtcgcgc gtctgcgcga tctgggtatg aaaggccagc aattcgtcgg
     3001 caccgtgcgc cactgttcgt tgctcggaaa cgggcacccc gaacgcgtac agggcgtcca
     3061 gtatcgcgct ctgggtccca ggcagcgccc agccatcgac ttcgcccagt ccgtaggcat
     3121 aacagaacaa cgggcggctt gcggtaatac gtgagtcgag ttggcgcacg aatccggccg
     3181 ctgcattgcg cggattaaca aataccttct cgccggccgc gcgctggcgt tcgttgagct
     3241 tctcgaattc ggcgcggcgc ataaaaattt caccgcgcac ctccagcacc gccggcggat
     3301 gatcggcgcg cagccgcagc gggatgctgc ggatggtttt cacgttcgag gtgacgtctt
     3361 ctccgatctc gccgtctccg cgtgtcgccg cgcgcaccag caccccgctt tcgtagcgca
     3421 ggctgatcgc gatgccatcg aatttcagtt ccgcgttgta ttcgatcgga ggatcatccg
     3481 gaccgagttt cagttcgcgg cggatgcggg tgtcgaatgt gagggcgccg ccgtgcgaat
     3541 cggtttcagt acgtacagac aacattggca ccgtgtgccg gactttcggc agatcggaac
     3601 gcacttcgcc acccacccgc tgcgtcgggg aatcgggcgt agccagttcc ggatactgaa
     3661 gttcgagtgc ctgcaattcg cgaaacagcc ggtcgtactc ggcgtccggc atggaaggcg
     3721 tgtccagcac gtaatagttg tagttcgcag cgtcgatgtc gcgccgcaac cgggccgcac
     3781 gctcggctgc cggcgacggc accgccatat caggagaaga ggcgcaacgc gagcggactg
     3841 ccggcgtcga ttccgcgttg ctccatcgcg ctatagatcg ctcgcagttg cgcgcggatg
     3901 cggtccagcc cggcatcgtt caacaacgcg cgattgtcgt ccgcgagaat gccgtccaaa
     3961 gattcggcga agctgcggct catggcaacc atccggtcga acacctgcaa gccattcgca
     4021 acgcgcggga catcgagcaa aaacgttact ccgggcgtgg tgatattgcg gatgcggtct
     4081 atcaggaaag gctccgactg ctggttatcc agcgagaaga gcgtactgcc ttgctcatca
     4141 tggcaatgga acacgccgct ggactgaagg cgcaatccgg aggcttctgc cagccccctg
     4201 atcttggtgc catggaaaac ctgaccggta ttggcaatta cgttgatccc gaccagcacg
     4261 tcgacgtcgg cgcaaaactc atcgagcgcg gcagcctgct cgagcgaagg tgcaaattct
     4321 ggcaattccg cgatggcgga caattcgctc gccgcagttt tcacgaagtc ggcaaacgtg
     4381 cggagctgtt cttccttcgc tggaccggag cggtccacga gctgaaggcc cagccgcaag
     4441 gtggtatagc gcccgtcagt cctcaagggt tcccatgatt tcgattcgtg attgagcccg
     4501 cctaaagtga tccttcggcc aagtgccgga atccgatgcc tgacttttgc aatttggtgg
     4561 gctccaatga actcgcctgc cctgagttca gcgatgtaat cgatttcccg acg
//
LOCUS       DATMZQ010000037         8744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_372919, whole genome shotgun sequence.
ACCESSION   DATMZQ010000037 DATMZQ010000000
VERSION     DATMZQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372919"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VN496_01285"
     CDS             complement(<1..463)
                     /locus_tag="VN496_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXF15602.1"
                     /translation="MEALAAPAQGMLDQVIACVREVAAREVMPRYLKVAHQRKSDGSL
                     FTEADLAAQEALSRVLPTIYSVPMVAEEMTPEQQAEYWLAGAEGLWCVDPIDGTSNFV
                     NGLPYFAVSVALMIRGRSVLGVVYDPIADEVFYAEKGAGAFLNGEPLPIKEH"
     gene            complement(451..999)
                     /locus_tag="VN496_01290"
     CDS             complement(451..999)
                     /locus_tag="VN496_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXF15603.1"
                     /translation="MRPQLPVCDFGWKAVDFDLPATDGKRYTLADVRSEKGLLVMFIC
                     NHCPYVKAIRERLIRDCKELSGLGIAAVAVNSNDPADYPEDSFANMQRVAREFAFPFL
                     YLFDETQAVARAYGAVCTPDFFGFNSELQLQYRGRLDASRTAPVANARRDLFEAMKSV
                     AETGRGPAEQIASVGCSIKWKR"
     gene            1098..1862
                     /locus_tag="VN496_01295"
     CDS             1098..1862
                     /locus_tag="VN496_01295"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HXF15604.1"
                     /translation="MDRDLDRKTTPKAGSRFHYHGNLGNGSEVKLQAEASHHAVRVLR
                     LAVGDPVALFDGRGGEFEARITRIDRGEVSVKTGVHRPVERESGLNIRLVQGLSSGDR
                     MDITLQKAVELGVTTIQPVSTERSIVKLKEERAQRRMEHWQSLVVSACEQCGRNRVPE
                     VLPLLDFPSWLAQLTMADEMRLMLSPSASMHLKELPAPVRLMLLIGPEGGLSPVEMQL
                     AQSRGFQPIRLGPRILRTETAALAALSAVQILWGDF"
     gene            1918..2685
                     /locus_tag="VN496_01300"
     CDS             1918..2685
                     /locus_tag="VN496_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXF15605.1"
                     /translation="MEIKNSTFIVTGGSSGLGAATVEMVVAAGANAIIADVNKSQGEA
                     LAAKLGKHARYVECDVTREDHGKGAVAAALKEFGSLQALVNCAGIAIGEKTVGKDGPH
                     SLEKFAKVININLVGSFNMIRLAADAMSQLQPNAAGERGVIVNTASVAAFDGQIGQAA
                     YSASKGGIVGMTLPIARDLSRSGIRVMTIAPGIFETPMLLGMPKDLQDALGKMVPFPS
                     RLGRPSEYAQLVRHIVENEMLNGEVIRLDGAIRMQPK"
     gene            2701..4020
                     /gene="argA"
                     /locus_tag="VN496_01305"
     CDS             2701..4020
                     /gene="argA"
                     /locus_tag="VN496_01305"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HXF15606.1"
                     /translation="MQAAQQANFVRWFRAAAPYIHAFGGRTFVIAFGGEVVAEGSFVA
                     LTHDLNLLASLGVRLVLVHGARPQIEAQLKAAGRKPQYVDGIRVTDEEALASVKQASG
                     QIRVEIEALLSMGLPNSPMANADIRVASGNFVTARPRGVVDGVDMCYTGEVRKVDADA
                     INTRLDDGELVLLSPLGYSPTGEIFNVTLEDVAAQTAIALAAEKLVFLMNEPGLKAKS
                     GRLLRELTVSDAEKLLRKGQSLSDDARLYLPHVIKACKGGAARVHLISRHADGALLLE
                     LFTHDGIGSMVTRDPLEQLRDATISDVGGILRLIEPLEADGTLVKRSRELLEMEISRF
                     TVLEHDKIIVGCAALYPFMNEKSAELACVAVHPDYRDSGGGEQLLKWIEEKAKKQRVK
                     RLFALTTRAAHWFIEHGFVETDISSLPKQKQALYNYQRRSKVLIKSV"
     gene            4171..4449
                     /locus_tag="VN496_01310"
     CDS             4171..4449
                     /locus_tag="VN496_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HXF15607.1"
                     /translation="MARMVNCVRLGREAEGLDFPPFPGTLGKRIFENVSKEAWKGWLE
                     HQKMLVNENRLSLADAKARKYLLEQTENYFFGQGADSAAGYVPPSRAS"
     gene            4608..6701
                     /gene="ppk1"
                     /locus_tag="VN496_01315"
     CDS             4608..6701
                     /gene="ppk1"
                     /locus_tag="VN496_01315"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXF15608.1"
                     /translation="MVADKSGKVRRSNPQLYLNRELAQLEFNRRVLAQAEDRELPLLE
                     RLRFVCIVSNNLDEFFEIRVAGLKAQIELGSDFTGPDGASPQHVFREVSLRAHELVAR
                     QYQLWNEELVPQLAGEGIRFLRRNDWNDEQKTWIKAFFLKEIMPVMTPIGLDRAHPFP
                     RILNKSLNFAVQLSGKDAFGRDSGFALVQAPRLLPRVIRLPRELAGTDHGFVFLSSII
                     HAHVGELFAGMEVQGCYQFRVTRNSDLFVDEEEVKNLRLALQGELPQRHFGDAVRLEV
                     ADLCSPAISDFLLQQFNLSAQDLYQVNGPVNLVRLMQVPDWVDRPDLKFPGFIASFPP
                     ALTKVPDLFAAMRKGDILLHHPFQSFRPVISFIQQAATDPNVVSIKQTVYRTGANSEL
                     MQTLLEAARNGKEVTVIVELMARFDEEANINWAAKLEEVGAHVVYGVVGHKTHAKMAM
                     VVRREEDRLRRYVHLGTGNYHARTAMLYTDFGLFTCHEEIGADVNDIFMQLTGLGRAG
                     KLNYLWQSPFTLHAQILAAIRHEAENAKAGTPAAITAKMNALLEPEIISALYEASMAG
                     VQIDLIVRGVCALRPGIPGLSDNIRVRSVIGRFLEHHRVFYFSSADTVLLSSADWMDR
                     NFFRRIEVCFPVLDEKLKKRVVDESLKAYLADSGEAWEMDGDGRYTRKKIAHHKAAAA
                     QAALLDKLSSGAAPD"
     gene            6732..7238
                     /gene="sixA"
                     /locus_tag="VN496_01320"
     CDS             6732..7238
                     /gene="sixA"
                     /locus_tag="VN496_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HXF15609.1"
                     /translation="MATAETISSLANMHGDGSMHLVLWRHAEAADGVPDMNRPLTAKG
                     KKQARQVAEWLSARLPKHTRILVSPARRALQTAEALTDEFDIVNELAPGATPAALIAA
                     SGWPDRNGAVLLVGHQPTLGLLASTLIAGEAMSWSIKKGAVWWISHRARDEPPKARLR
                     AVIAPDFV"
     gene            complement(7389..>8744)
                     /locus_tag="VN496_01325"
     CDS             complement(7389..>8744)
                     /locus_tag="VN496_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HXF15610.1"
                     /translation="SRAIDCLKRFGERLRGLPAGAVRAVGTNTLRVARNAREFVPQFE
                     AALGFPIEVVAGREEARLIYLGVAHSLPASQETRLVVDIGGGSTECIVGAGNQPHRLE
                     SLYMGCVSYSLKFFADGKIGKSDMKHAETAARIELETIRKQFSRKHWQQAVGSSGTAR
                     AIGDIIEASGWSTSGITRDGMERLRQALLKAGDTAKLDLPGLKDDRVAVLPGGFAIMN
                     AIFAELDVEHMALATGAMRQGILWDMIGRAHRRDMRELTVRQFMKRYHVDAGQAHRIE
                     RMALKLYGQLVDMDRHERDYALHMLSWAARLHEIGLTVAHSGYHKHSSYIVANADMPG
                     FSKMEQGQLSMLVLAHRGSLGKLRGLVRADYDWSLLVALRLAALLHRSRSDVRLPAIR
                     PSRKKSVFTIEVDARWLAANPLTAAELKEEIRDWKGLGVELSVPQLAEVESVGETAAT
                     E"
BASE COUNT         1875 a         2572 c         2554 g         1743 t
ORIGIN      
        1 cgtgttcctt gatgggcagc ggttccccgt taagaaacgc gcccgccccc ttttcggcgt
       61 agaacacttc atccgcgatg ggatcgtaca ccacgcccaa cacgctccgc cccctgatca
      121 ttaatgccac ggaaaccgca aagtagggca ggccattgac gaagttggaa gtgccgtcga
      181 ttggatcgac acaccacaat ccctccgcgc ccgcgagcca gtattccgcc tgctgctccg
      241 gcgtcatttc ttcggccacc atcggaacgg aatagatcgt gggcagaacg cgcgacaacg
      301 cctcttgtgc agccaaatcc gcctcggtaa acaggctacc gtcggattta cgctgatggg
      361 ccactttcaa atagcggggc atgacttccc tggccgctac ctcccttacg caggcgataa
      421 cctggtccag cattccttgc gccggcgcgg ctagcgcttc catttaatgg agcatcccac
      481 gctggcaatc tgctcggctg gaccacgacc cgtctcagct acggacttca tggcttcgaa
      541 cagatctcgt ctggcgtttg cgaccggcgc agtccgcgag gcatccaggc ggccgcgata
      601 ttgcaattgc agctcgctgt tgaagccgaa gaaatccggc gtgcaaaccg cgccataggc
      661 gcgtgcgacg gcttgcgttt catcgaacag gtacaggaaa gggaacgcga attcgcgcgc
      721 gacccgctgc atgttcgcga atgaatcctc cggatagtcc gcgggatcat tggaattgac
      781 ggcaactgca gcgatgccca gcccggaaag ctccttgcag tcccggatca ggcgctcgcg
      841 tatggctttg acataagggc agtgattgca gatgaacatc accagcaatc ctttttcgct
      901 gcgtacatcg gccagcgtgt atcgtttgcc atccgtagcc ggcaagtcga agtcaaccgc
      961 cttccagccg aaatcgcaca ccggcaactg cggccgcatt cgtcagaccc tttgcctggc
     1021 acgttgcgcc gggggcaacg ttgaaggcct ttgatgaact gggagacagt cccgattgtg
     1081 catggcaaaa tcttagcatg gatagagact tagacagaaa aacaacccct aaagcaggtt
     1141 cgcgatttca ctaccacggt aacctcggca acggttcgga ggtgaaattg caagcggaag
     1201 cctcccatca tgcggtacgc gtattacgcc tcgcggtggg tgaccctgtc gcattgttcg
     1261 acggccgtgg cggcgaattc gaggccagga tcacacgcat cgatcgcggc gaagtctccg
     1321 tcaaaaccgg agtgcatcgg cccgtggaac gtgaatccgg cttgaatatc cggctggtgc
     1381 agggcctgtc gagcggcgat cgcatggata tcaccttgca aaaggccgtt gagcttggcg
     1441 tcacaacaat ccagccggtg tccacggaac gcagcatcgt aaagttgaag gaggagcgcg
     1501 cgcaacgccg catggagcat tggcaaagcc tcgtcgtttc agcatgcgag caatgcgggc
     1561 gtaatcgtgt gccggaagtg ctgcccctgc tggacttccc ctcctggctg gctcagctca
     1621 ccatggcgga tgaaatgcgg ctaatgctgt ccccttctgc aagcatgcat ttgaaagaat
     1681 tgccggcacc cgtgcgcctg atgctgctga tcggtccgga aggagggctt tctcccgttg
     1741 aaatgcagct ggcccaatcg cgcggatttc agccaatccg tcttggaccg cgcatcctgc
     1801 gcaccgagac cgccgcgctt gctgcgctgt cggcggtcca gatactgtgg ggcgattttt
     1861 aaatactcgc tgctatactc cgggtccctt tatttcgtag cgaaacaaac gctaagcatg
     1921 gaaatcaaga acagcacgtt tatcgtcacc ggtggcagtt caggcctcgg tgcggccacc
     1981 gtggaaatgg tggttgctgc cggtgcaaac gcaatcattg ccgatgtcaa caaatcgcag
     2041 ggcgaagcat tggccgcaaa gctgggcaag catgcacgct acgtcgagtg cgatgtcaca
     2101 cgcgaagatc acggcaaagg agctgtcgct gccgcactga aggagtttgg cagtctgcag
     2161 gcgctggtca actgtgcagg gattgccatc ggggaaaaaa ctgtaggcaa ggatggcccg
     2221 cattcgctgg agaagtttgc aaaggtcatc aatatcaatc tagtgggcag tttcaacatg
     2281 atccgcttgg ctgccgacgc aatgtcgcag cttcagccga acgccgctgg tgagcgcggt
     2341 gtgatcgtca acacagcgtc ggtcgccgcc tttgatgggc aaatcggtca ggcggcctac
     2401 tcggcttcca aaggaggcat tgtcggcatg acgctgccga ttgcgcgaga cttgtcccgc
     2461 agcggcatcc gcgtcatgac cattgcgccg ggcattttcg aaacgccgat gctgctgggt
     2521 atgcccaagg atttgcagga cgcactgggg aaaatggtgc cgttcccttc acggctgggc
     2581 cgcccatccg aatatgcgca gctcgtcagg cacatcgttg aaaacgaaat gctgaacggc
     2641 gaagtcatcc ggcttgatgg cgccatccgc atgcaaccga aataagcacc ggctgccgac
     2701 atgcaagccg cccagcaagc aaacttcgtc cgctggttcc gtgccgcagc gccgtatatc
     2761 catgcgttcg gcgggcgcac ctttgtcatt gccttcggcg gagaagtcgt ggcagaaggt
     2821 tcgttcgttg cactgacaca cgacctgaat ctgctcgcca gtctcggcgt tcgcctggtt
     2881 ctggtacacg gggcacggcc gcagattgaa gcgcaactga aagcggccgg gcgcaagcca
     2941 cagtatgttg atggcattcg ggtaaccgat gaagaagcgc tggcttccgt gaagcaggcg
     3001 agcgggcaaa tacgcgtcga aattgaagca ctgctgtcca tggggctgcc gaattcacct
     3061 atggcaaacg ccgatatccg cgtcgcctcg ggaaatttcg tcacggcacg gccgcgtggc
     3121 gtcgtcgacg gcgtcgacat gtgctacacc ggtgaagtcc gcaaagtgga tgcggatgcc
     3181 attaataccc ggctggatga tggcgaattg gtgctgctgt ccccgctcgg ctactcgccg
     3241 acgggggaga ttttcaatgt caccctggaa gatgttgctg cccaaactgc catcgcgttg
     3301 gcagcagaaa agctggtgtt cttgatgaac gaacccggat tgaaggcaaa aagcggacgc
     3361 ctgctgcgcg aactcaccgt atcagatgcg gaaaagctgc tgcgcaaagg acaatccctg
     3421 tctgacgatg cgaggcttta cctgccccat gttatcaagg cctgcaaagg cggcgctgca
     3481 cgcgtccacc tgatcagtcg ccacgccgat ggcgcgcttc tactggaatt gttcacgcac
     3541 gacggcatcg gcagcatggt cacacgcgat ccgctcgagc agttgcgcga tgccacaatc
     3601 agcgacgtgg gcggcatcct gcgccttatc gaaccactcg aggcagacgg aacgctggtt
     3661 aaacgctctc gtgaactgct cgaaatggag atatcgcgct ttaccgtgct ggaacacgac
     3721 aaaatcatcg tcggctgcgc tgccctgtac cctttcatga acgaaaagtc ggccgaactg
     3781 gcctgcgtgg ctgtgcatcc cgactatcgg gattctggcg gcggagaaca actgctgaaa
     3841 tggatcgagg aaaaggcgaa gaagcagcgc gtcaagcggc tattcgctct taccacgcgc
     3901 gctgctcact ggttcattga acatggcttt gtggaaaccg acatcagcag cctgccgaaa
     3961 cagaagcagg cgctatacaa ctaccagcgc cgttccaagg ttttgatcaa gtcagtctaa
     4021 tcggggcggc cggcctgcct cccagcgaac aggaatcggt ggtctataat tgaggcgggc
     4081 gttgttgcgg aattcgagcg tttgcggatt gtcacaaatc cgtaaccccg caacgcgcgc
     4141 aatcaatcta aagcttcccg acagacaaat atggcgagaa tggtgaactg cgtaagactt
     4201 ggccgggaag ccgagggcct cgattttccg ccttttcccg ggacactcgg caagcggatt
     4261 tttgaaaacg tgtccaaaga ggcgtggaag ggctggctcg aacaccagaa aatgctggta
     4321 aacgaaaacc ggctcagtct cgccgacgca aaggcgcgca aataccttct ggaacaaact
     4381 gaaaattatt ttttcggaca gggggcggac tcggccgcgg gctatgtccc tccctccagg
     4441 gctagctgaa tcggctcgcc gaactggctg gccaacgctg ccctgatcca caactaaaac
     4501 aagacagcgg agcaagaaaa attaccaaag cggggcaaac caagacggat tctcccgacc
     4561 gcgcagcaac acttgcgccc gaaaaggcgg ccaggaaaag agccgccatg gtcgctgaca
     4621 agtcgggcaa ggtcaggcgc tccaatcccc agctctatct gaaccgggaa ctcgcgcaac
     4681 tcgagttcaa ccgcagggtg ttggcgcagg cggaagaccg tgaattgcca ttgctggagc
     4741 gcttgcgctt tgtgtgcatc gtcagcaaca accttgatga atttttcgaa atccgagttg
     4801 ccggcctgaa agcgcagatc gaactcggct ctgactttac aggtccggat ggcgcttcgc
     4861 cgcagcatgt tttccgtgaa gtctcactgc gggctcacga actggtcgcg cgccagtatc
     4921 aattgtggaa cgaggaactg gtccctcaac ttgcgggcga aggaatccgt ttcttgcgcc
     4981 gcaacgactg gaatgatgag caaaagacct ggataaaagc tttcttcctg aaagaaatca
     5041 tgccggtcat gacgccaatc gggcttgacc gggcacaccc ctttccccgc attctgaaca
     5101 aaagcctgaa cttcgccgtg caactgtcgg gcaaagacgc tttcgggcgc gactcgggtt
     5161 tcgcgctggt gcaggcgccg cgcctgctcc cacgcgtcat ccggcttccg cgtgaactcg
     5221 ccggaacgga tcacggtttt gtgttcctct cctcgatcat ccacgcacat gtgggtgagc
     5281 tgttcgccgg catggaagtg cagggctgct accagttccg cgtcacgcgc aatagcgacc
     5341 tgttcgttga cgaagaagaa gtcaagaatc tgcgcttggc gttgcaaggc gaattgccac
     5401 agagacattt cggcgatgcg gtgcgccttg aagtggccga tctgtgctcg cccgctattt
     5461 ctgattttct gttgcagcag ttcaatctca gcgctcagga cctctaccag gtaaacggcc
     5521 cggtcaatct ggtccgcctg atgcaggttc cggactgggt tgatcgccca gatctgaaat
     5581 tcccgggctt catcgcaagc ttcccgccgg cactgaccaa ggtgccggat ctctttgcag
     5641 cgatgcgcaa aggggacatc ctgctgcatc atcccttcca gtcgttcagg cccgtgatca
     5701 gcttcatcca gcaagcggct actgacccga acgtggtttc gatcaagcaa acggtctacc
     5761 ggaccggagc caactccgaa ctcatgcaaa cgctgctcga ggctgcgcgc aatggcaaag
     5821 aagtgaccgt aatagttgaa ttgatggcgc ggtttgatga agaggcaaac atcaattggg
     5881 ctgcaaagct ggaggaagtc ggcgcgcatg tcgtctatgg cgttgtgggc cataagacgc
     5941 acgccaaaat ggcgatggtg gtgcgccggg aagaagaccg gttacgccgc tatgtccatc
     6001 ttggaaccgg aaattatcat gcgcgcaccg caatgcttta taccgatttt gggttgttca
     6061 cctgccatga agaaatcggc gctgatgtaa atgatatctt catgcagctc accggactgg
     6121 gccgggccgg gaagctgaat tatttatggc agtctccgtt caccctgcat gcacagatac
     6181 tggcggctat ccgccacgag gcggaaaacg ccaaggccgg gacgccggcc gcaattaccg
     6241 cgaaaatgaa cgccctgctg gaaccggaaa tcatttccgc gttgtacgaa gcctccatgg
     6301 ccggtgtgca gatcgacctg atcgtccgcg gcgtatgcgc gctgcggccc ggcattccag
     6361 gactgtccga taacatccgc gtgcggtcgg ttatcggccg cttcctcgaa catcatcggg
     6421 tgttctattt cagcagcgcc gataccgtgc tgctgtccag cgccgactgg atggaccgca
     6481 attttttccg gcggattgaa gtgtgcttcc cggtactcga cgagaaactc aagaagcggg
     6541 tggtggacga gtccctgaag gcctacctcg ccgatagcgg agaagcctgg gaaatggacg
     6601 gtgatgggcg ctacacgcgc aagaaaatcg cccatcacaa agcggcagcg gcccaggcag
     6661 cgctgctcga caagctttcc tcgggtgcag cgcccgattg aagatcggcg tctgacaggt
     6721 gaaaactgcg cgtggcaact gctgaaacga tttcatcgct tgcaaacatg catggggatg
     6781 ggtcgatgca cttggtgttg tggcgtcatg cagaagcggc agacggcgtg ccagacatga
     6841 accggccgct cactgccaag ggcaaaaagc aggcacgcca ggtcgctgaa tggttaagtg
     6901 cgcggctgcc aaaacatact cgtattctcg tcagtccggc gcgacgcgcg ctgcagacgg
     6961 cagaggcact tactgacgag ttcgatatcg tcaatgaact ggctccaggc gccacgccag
     7021 ccgcattgat cgccgcttct ggatggccgg accgcaacgg cgctgtcctc ctggtcggtc
     7081 atcagccgac gctgggcctg ctcgcgtcga ctctgattgc cggtgaagca atgtcctgga
     7141 gcatcaagaa aggcgccgtc tggtggatat cgcaccgggc gcgcgacgaa ccgcccaagg
     7201 cgcgactgcg cgcggtcatc gctcctgatt tcgtttagaa ggaccgctgt tcgagtcagc
     7261 gaagttacgc cgcgccatca gcaagcaatc gcacgcgcgc cgggtgccca agatggaagg
     7321 cgcgtgcgaa agcgttgaag gcaatctgtg agcgcctgga ttcggcactc cgcagaagct
     7381 tgggcttctc attccgttgc cgctgtttcc cccactgatt cgacttcggc cagttgcggg
     7441 acggacaact cgacacccaa tcccttccaa tccctgatct cttctttgag ctcggccgcc
     7501 gtgagtggat tggcggccag ccaccttgca tcgacttcga tggtaaacac ggattttttt
     7561 ctgctgggac gaatcgcagg caggcgcaca tccgagcgac tgcgatgcaa aagcgccgcc
     7621 aaccgcagcg ccacgagcag cgaccagtcg tagtcggccc gcaccagccc acgcagtttt
     7681 ccaagcgaac cacgatgggc cagcaccagc attgacagtt ggccctgctc catcttggaa
     7741 aagcctggca tgtcggcatt cgcgacaata tatgatgaat gcttgtgata cccgctgtgc
     7801 gcgacggtta ggccaatctc gtgcaaacgc gccgcccatg acaacatgtg cagcgcgtaa
     7861 tcgcgttcgt gcctatccat atcgaccagc tgtccataca gtttcagcgc catgcgttcg
     7921 atgcgatgcg cctgtccggc atccacgtga tagcgcttca tgaattgacg cacggtgagt
     7981 tcgcgcatgt cgcggcggtg ggcgcggccg atcatgtccc acaggatgcc ctggcgcatg
     8041 gccccggtag caagggccat gtgctcgaca tcgagctcag cgaatattgc attcatgatc
     8101 gcaaagccgc cgggcagcac ggccacgcga tcgtctttca atccgggcaa atccagcttg
     8161 gcggtatcgc cggctttcag cagcgcctgg cgcaggcgct ccatcccatc ccgcgtgatc
     8221 cctgaagtgc tccagccgct agcttcgata atgtccccta tcgcgcgtgc cgtgccggaa
     8281 gagccgaccg cctgttgcca gtgcttgcga gagaactgtt tgcggatagt ttcgagttca
     8341 atgcgtgcgg ctgtttcggc atgcttcatg tccgacttgc caatcttgcc gtcggcaaag
     8401 aacttgaggc tgtagctgac acagcccata tacaggcttt ccagccggtg tggttgatta
     8461 ccggcgccaa cgatgcattc ggttgatccg ccgccgatgt cgacgaccag gcgggtctcc
     8521 tgacttgcgg gcagactgtg cgcgacgccg agatagataa ggcgggcttc ttcccgcccg
     8581 gcgactactt cgatcggaaa accaagtgcg gcttcgaact gcggcacaaa ttcgcgcgcg
     8641 ttcctggcga cgcgcagcgt gttcgtgccg accgcccgca ctgcgccggc aggcaggccg
     8701 cgcaaccgct cgccaaaacg cttcagacaa tcgatagcgc ggga
//
LOCUS       DATMZQ010000038         3752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1395184, whole genome shotgun sequence.
ACCESSION   DATMZQ010000038 DATMZQ010000000
VERSION     DATMZQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395184"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VN496_01330"
     CDS             <1..224
                     /locus_tag="VN496_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-butanediol diacrylate esterase"
                     /protein_id="HXF15611.1"
                     /translation="KSWGFGFMINHDLAPTGRSAGSLAWAGLSNCYFWIDPMRRIAGV
                     YLTQILPFADKKSLPLYLDFETAVYRSAS"
     gene            228..1262
                     /locus_tag="VN496_01335"
     CDS             228..1262
                     /locus_tag="VN496_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXF15612.1"
                     /translation="MSEPAEDVAILRGIITQYFASRALYAAAALDVAGALSTGSKSIE
                     ELAQLIGAHASSLYRVLRVLAASQIFREEEDRRFANTALSELLRADVPGSLRDLVLLF
                     GDETSWRSWEGILHGVRTGEAPFEHIYGEKFFDFLQDHADTAAMFDRAMASASSTTNA
                     AVAQACDFSGFGTVLDIAGGTGSALCSILKAAPHLHGIVFDLPHVAQRAREFIAAQGL
                     TQRCEFSGGSFFEAVPSGADAYFMKHVLHDWGDAECAAILGNCRKAMSRDARLLICER
                     IVPPGNEPSSAKLIDLHMMMTNHGGKERTEPEYRDLLNKSGFELQRVIATGTPWSVIE
                     AQPVKIATAI"
     gene            complement(1264..2142)
                     /locus_tag="VN496_01340"
     CDS             complement(1264..2142)
                     /locus_tag="VN496_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HXF15613.1"
                     /translation="MRFVLVVAALCAGRAQAHTLENVRSSGLELWLACLLLTAAGLYA
                     WGVHRLWQAAGPNHGVTLYRIAAFAGGWTVLTLALAPPLDPMGAELFSAHMLQHELLM
                     LVAAPLLVAGSPLAVWAWAMPPRWRRSIACITHSRGIAAIWATLTAPLAAWILHAAAI
                     WLWHLPGLFTAALEHEGVHVLQHFSFLFSALLFWWAATRRTSSAGTAASVFYLFTTMV
                     HTGALGALLVFAPTLWYPVYGTAPLEWGLQPLEDQQLGGLIMWIPGGLVYAGAAIALM
                     ARWLQTHARSGLHRPA"
     gene            complement(2124..2417)
                     /locus_tag="VN496_01345"
     CDS             complement(2124..2417)
                     /locus_tag="VN496_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15614.1"
                     /translation="MLIAPCVLLLELTVAYAVVHLTCANGLRASVLHSVFAAALLVTL
                     LMLGLAWHSWHVATKGHERFLNQLSLALSALVILVIATQWVTVSILQPCVSSL"
     gene            complement(2437..3054)
                     /locus_tag="VN496_01350"
     CDS             complement(2437..3054)
                     /locus_tag="VN496_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXF15615.1"
                     /translation="MSRSGGDATQIVHASALPSHAFGHRSMMWWGVMGMIAIEGTVFA
                     LAIAVYLYVRKDFTEWPPVAQPPELFWGTLNTLILIVSMVPNQWAKRAAERYDARNVK
                     WALVVCLIFSAAFLAVRVFEFGSLNCRWDTNAYGSAIWMLLGLHTLHLLTDTYDTAVL
                     TALMWFGPVEDKRFVDVSESAVYWYFVVIAWLPVYAIIYFGARLL"
     gene            complement(3051..>3752)
                     /locus_tag="VN496_01355"
     CDS             complement(3051..>3752)
                     /locus_tag="VN496_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXF15616.1"
                     /translation="GGAVFPLFGAFYFWFPKITGRLMNEELGHWNFWLFFVGFNVAFF
                     PMHYLGLHGMPRRVYTYPADMGWGTLNFIATVGAMMIAVSVLLFIVNVVRSLRAGTSA
                     GPNPWGAATLEWAVSSPPPSYNFATIPVVGGREPLWEPGGINGRVEGLSCKLPELLIT
                     HVLDGSPDHRQLFPAPSIWPFVGAVATTVLFIASIFSPWAVVWGSIPLAIALTAWFWP
                     DRRETEEHQKLERRP"
BASE COUNT          874 a         1135 c         1080 g          663 t
ORIGIN      
        1 cgaaaagttg ggggttcggc ttcatgatca atcatgacct ggcgccaacc ggtcgctctg
       61 ccggaagcct ggcatgggca ggcctgtcga actgctattt ctggatcgat ccgatgaggc
      121 gcatcgccgg ggtctatcta acccagatcc tgccgttcgc ggacaagaaa tcgttgccgc
      181 tgtacctgga ttttgaaact gcggtctacc gttcggcaag ctgagccatg tccgaacctg
      241 cggaagatgt tgcgatattg cgcggcatta tcacgcagta ttttgcctcg cgagcgttgt
      301 atgctgccgc tgcactggac gtcgccggcg cattgagcac ggggagcaag agtatcgaag
      361 agcttgcgca gctcatcggc gcgcatgcgt cgtcgctcta ccgggttttg cgcgtattgg
      421 ctgcatcaca aatctttcgg gaggaagaag accgtcgatt tgcaaacaca gcgctatcag
      481 agctgctgcg tgctgatgtg cccggttcac tgcgcgacct ggtattgttg ttcggcgatg
      541 agacctcatg gcgctcgtgg gaggggattc ttcatggggt gcgtactggc gaggcgccat
      601 tcgagcatat ctacggcgaa aaattcttcg atttcctgca ggaccatgcc gatactgcgg
      661 cgatgtttga ccgtgccatg gcgagcgcat catccactac taacgcggca gtcgcgcaag
      721 cctgcgactt ctccggcttc ggtacggttc tggatattgc cggcggaacg ggttccgcgc
      781 tttgcagcat cctgaaagcc gctccgcatt tgcacggcat cgtatttgat ctgccgcacg
      841 ttgcgcaacg cgcgcgcgag ttcatcgcgg cgcagggctt gacgcagcgc tgtgaatttt
      901 ccggaggcag tttctttgaa gcagtgccaa gcggcgccga cgcctacttc atgaagcacg
      961 tcttgcacga ttggggcgat gcagaatgcg ccgcgatact gggcaattgc cgcaaggcaa
     1021 tgtcgcgcga tgcacggctg ctcatttgcg aacgcatcgt gccacccggc aacgagccct
     1081 cgtcggccaa gttaatcgat ctgcacatga tgatgacgaa ccacggcggc aaggagcgca
     1141 ccgaacccga ataccgtgat ttgctcaaca aatccggttt cgaattgcaa cgtgtgattg
     1201 ccaccggcac gccgtggagc gtgatcgagg cccaaccggt gaagattgca acggcgattt
     1261 agcctaagct ggccggtgca agccggaccg tgcatgcgtt tgcaaccagc gtgccatcag
     1321 cgcaatggcg gcgcccgcat agaccagacc gcccggaatc cacatgatca acccgccgag
     1381 ttgctgatct tccagcggct gtaggcccca ctctagcggc gcggttccat acaccgggta
     1441 ccagagggtc ggcgcaaata ccaacagcgc acccagcgcg ccggtatgca ccatggtggt
     1501 aaacaaataa aacacggagg ccgcggtacc agcggacgat gtcctgcgtg ttgccgccca
     1561 ccagaacagc agcgccgaaa acagaaagct gaaatgctgc aggacatgaa cgccttcatg
     1621 ttccagggcg gcggtgaaca acccgggaag atgccaaagc cagatagccg ccgcgtgcag
     1681 gatccaggcc gcaagcggcg cggtcaatgt tgcccaaatc gcggcaattc cgcgggagtg
     1741 cgtgatgcat gcgatcgagc gccgccagcg cggcggcatc gcccaagccc aaactgccaa
     1801 aggactcccc gccaccaaca gcggtgcggc gaccagcatc aacaattcgt gctgcagcat
     1861 atgtgcactg aacagctccg cgcccattgg atctagtggc ggggcaaggg cgagtgtcag
     1921 cacagtccaa ccgcccgcaa aagcagcgat ccgatagagc gtcacgccgt gatttggtcc
     1981 ggctgcttgc caaaggcggt gcactcccca tgcatacagc ccggctgctg tcagcagcag
     2041 gcacgccagc cacaactcca ggcccgaaga acgtacgttt tccagcgtgt gcgcctgcgc
     2101 ccgtccggcg cacagcgccg ccactacaag gacgaaacgc atggctgaag gatgcttacc
     2161 gtgacccatt gcgtcgcgat gaccaatata acgagcgcgc tcagtgcaag actcagttga
     2221 ttcagaaagc gctcatgccc tttagtcgca acgtgccaag aatgccatgc caaaccaagc
     2281 atcagcagcg taacgagcaa cgcagcagca aataccgaat gcaaaacgga ggcacgcagg
     2341 ccattcgcgc acgtcaggtg caccacggca taagcaacgg tcaattcgag cagcaatacg
     2401 caaggggcga tcagcatgcc gaccgacacc gatcccctac agcaggcgcg cgccgaaata
     2461 gatgatcgca tagacgggca accaggcaat gacaacgaaa taccagtaca ccgcgctttc
     2521 gctgacatcg acgaagcgct tgtcttctac cggaccgaac cacatcagcg cagtcaatac
     2581 cgcggtgtcg taagtgtcgg tcagcaagtg cagggtgtgc aggcccagca gcatccagat
     2641 ggcggatccg taggcgttag tatcccagcg gcagttcaag ctgccgaact cgaatacccg
     2701 caccgccagg aacgccgctg aaaaaatcag gcagacaacc agcgcccatt taacattacg
     2761 ggcgtcatag cgctcggccg cgcgcttcgc ccactggttg ggaaccatgc ttacaatcag
     2821 gatgagcgta ttcagcgttc cccaaaacag ctccggcggc tgtgcaaccg gcggccattc
     2881 ggtgaaatcc ttgcgaacgt atagatacac tgcaatcgcc agcgcgaaga ccgtcccttc
     2941 gatcgcaatc atgcccatca caccccacca catcatgctg cgatggccga aggcatggct
     3001 gggaagcgcg gaggcatgga caatctgtgt tgcatcacct ccagaacgac tcacggacgg
     3061 cgctccagtt tttgatgttc ttcggtttca cggcggtccg gccagaacca ggccgtgagc
     3121 gcaatagcta acggaatgct gccccaaacc accgcccagg gagaaaagat actggcgatg
     3181 aacaaaaccg tcgtggcgac tgcgcccacg aacggccaga tggatggagc cgggaacagc
     3241 tgacggtgat ccggtgaacc gtcgagtacg tgggtgatca gcaattcggg caacttgcag
     3301 gacaagcctt ccacgcgtcc gttgatacca cccggttccc ataggggttc gcgaccgcca
     3361 acaaccggga tggtcgcgaa attgtaggac ggcggaggag aagagaccgc ccactccaat
     3421 gttgccgccc cccatggatt agggcccgcg ctggtgccgg cacgcaggct gcgaaccacg
     3481 ttgacaatga acagcaggac gctcactgca atcatcatcg cgcccaccgt agcaatgaaa
     3541 ttcagtgttc cccagcccat atccgcagga taggtatata cgcgccgcgg catgccgtgc
     3601 agccccagat aatgcatcgg gaagaatgca acattgaagc cgacaaaaaa cagccagaaa
     3661 ttccagtgcc cgagttcttc gttcatgaga cgtccggtaa ttttcgggaa ccagaaatag
     3721 aatgcaccga acagcggaaa taccgcgccg cc
//
LOCUS       DATMZQ010000039         6315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3135632, whole genome shotgun sequence.
ACCESSION   DATMZQ010000039 DATMZQ010000000
VERSION     DATMZQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135632"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VN496_01360"
     CDS             complement(<1..1529)
                     /locus_tag="VN496_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF15617.1"
                     /translation="MSSRMIVLVIAAIAAFSIAGYGLYVVGMNRGLKMATVENQPAPD
                     AKNKGRPGSDKKVLYWHDPMVPGQKFDKPGKSPFMDMPLVPVYAGEEGDEGKVAISPR
                     MQQNLGVRTATVISGALENSVQAVGTVAYNERDVAVVQARSNGFLEKLYVRAPLDAVR
                     KGQALAELYVPDWVAAQEEYLSAKRISASGLQDVLTDLTEGARQRMRLAGMTEDQIRL
                     VETTGKVQATVTIVAPINGVIAELGAREGMTVTSGATLFRINGLNTVWVNAEVPESQM
                     ALVHPGTQVTAVSASIAGVTFKGKVNALLPEVNAATRTIKARIELANENGQLVPGMFV
                     TVRLAPAQRKEALLVPAEAVIRTGTRSVVIVALEDGKFTATDVETGTEAQGQTEIRRG
                     LNKGDKVVVSGQFLIDSEASLKGATSRMGEAPAAPPEIGSSRTHRGQGKVEKIGEHEI
                     MLSHGPIPSLQWGAMTMGFGIPAGGLPKIISVGDTVAFEFHQTSDGGYELTSIAPLPV
                     AA"
     gene            complement(1526..2920)
                     /locus_tag="VN496_01365"
     CDS             complement(1526..2920)
                     /locus_tag="VN496_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXF15618.1"
                     /translation="MQSSTFPLAQACSAVSQYSKAALPTIVAQFCGNAVLIFFATILV
                     ALCADLVLVPGEALADEAPLSIQEAQRRAVERSRQIAAQNASVTASREMAVAAGQLPD
                     PVLRFGIDNLPINGPDQFSVTNDFMTMRRVGVMQEFTRSDKRELRAQRFENEAEKSLA
                     EKEAVIASIQRESALAWLDVYFAEAQAKVIAQQNAQAKIEITAAEAAYRAGRGSQADV
                     FAAHSARATLEDQASEVGRRVANARTMLARWVGDEPAFSISEKPAMDSIRVDSSTLDM
                     ELGHHPQIAILTKREQIAATEVRLAQADRKADWSVELSYSLRGPAYSNMVSIGVSVPL
                     QWDQRNRQERQIASKLAAAEQARAEREEVLRAHVSEVRTMIAEWQNGRERVARYEREL
                     LPLALERTKAARAAYQGGKSTLTELLLARRNEIDVRMQKVRFEAETARLWAGLNFLFP
                     DFASPSNANDGASK"
     gene            complement(2984..3376)
                     /locus_tag="VN496_01370"
     CDS             complement(2984..3376)
                     /locus_tag="VN496_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15619.1"
                     /translation="MTSIFGMRNSGRRLGTMLCRFVLGVMVFAQVANASQSCVAPEMS
                     PIMAFSQDKQDDHCHKGVNPNSCLQQANASDQSLDHAQTPVFATSSVAILVLPWATDC
                     AVPGAVLTAASSPTTDPPPLIRFCSLQL"
     gene            complement(3461..4687)
                     /locus_tag="VN496_01375"
     CDS             complement(3461..4687)
                     /locus_tag="VN496_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXF15620.1"
                     /translation="MSSILDLALGIMSALGGFVDIGELVFTTQGGAKFGYMLIWTVVV
                     GTIGIMVYGEMAGRIAAVLQKPTFQIIRERVSYRSGLMTLVASNIVNLLTCAAEIGGV
                     AIVLQLLFGLEYRLMLITAVGLLCIAVYFMKFEWLERLFGLMGLALLVYAAAAVALKP
                     DWSAVGKGFLPQLPQDNVLVYFYYVVGLFSSIVMPYEIYFYSSGAIEDKWKAKDLPTN
                     VLTATVGFTLGGLLSTALIVAGATLFGSHGVIPDTLSATALPATAAFGIKGLLLALLG
                     MLFAIGGAAVETALAGAYNVAQFFGLAWGKDKNMKQAPVFHATWLVLIGSGFAIAMSG
                     INPLLIVEYSVVFAVVVLPFTYYPILRTARDKKLMKGHANSPLITAAGWLYFAIITVA
                     AVSAIPLMIVTHMGEG"
     gene            complement(4684..5136)
                     /locus_tag="VN496_01380"
     CDS             complement(4684..5136)
                     /locus_tag="VN496_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15621.1"
                     /translation="MPYQIKKWVAIVFGTTTVLVIAVVLAKIASTEPEPMPVEEFGRH
                     VRALGSIAGEIVLFSTQLEQGRLTDNYAKVHCEKLQEELRSRARQLDGPVPPEMAEAG
                     TRVRNSAEALSAMWQELSRHRTDNPEILKIKQQAMRLEQQLGNLETAL"
     gene            complement(5319..5789)
                     /locus_tag="VN496_01385"
     CDS             complement(5319..5789)
                     /locus_tag="VN496_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15622.1"
                     /translation="MNPRRLLQNPEFNLGVFAFLLNLVWEFWQIPFFVGMADQPQWLG
                     VKICTQTTLGDVAIILVAFWITAIAARNRNWILLPRKEDLALFLGIGIIATALVEGVA
                     TGPAGRWSYTLAMPRLPFIGTGLLPILQWLVIPPLVLWFARRQINGDPHRRRPD"
     gene            5989..>6315
                     /locus_tag="VN496_01390"
     CDS             5989..>6315
                     /locus_tag="VN496_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HXF15623.1"
                     /translation="MNDQMRSCIDACYACAIACDECAVSCLKEEDFPHLARCIQLDMD
                     CADMCRLAMAYMGRDSELINLVCHTCAEICDACAEECAHHNTMDHCKACEEACRRCAQ
                     ECRKLIA"
BASE COUNT         1338 a         1892 c         1718 g         1367 t
ORIGIN      
        1 gcagccaccg gcaaaggggc tatggaagtc aattcatagc ctccatcgct ggtctggtgg
       61 aattcgaacg caacggtatc gccgactgaa attatttttg gcagacctcc ggccgggatg
      121 ccgaaaccca tggtcattgc accccattgc agcgacggga ttggaccgtg cgagagcatt
      181 atttcgtgct cgccgatttt ctcgaccttg ccttgcccgc gatgcgtccg gctgctgccg
      241 atttcgggag gcgccgccgg agcctcgccc atccggctcg ttgcgccttt cagacttgcc
      301 tccgaatcga tcaggaactg gcccgacaca accactttgt cgcctttgtt caaaccgcgt
      361 cgtatctcgg tttgcccctg ggcttccgtt ccggtttcca cgtctgtggc agtgaattta
      421 ccgtcctcaa gcgcgacaat gaccacactt cgcgtgcccg tgcgaatcac tgcttcagca
      481 ggcaccagca gcgcttcctt gcgctgtgcc ggtgctagtc tcactgttac gaacatgccc
      541 ggcaccagct gaccgttttc attcgccagc tcgatgcgcg ctttgatggt cctggtggcc
      601 gcgtttactt ccggcagtaa ggcatttacc tttcccttga atgtgacccc agcgatcgag
      661 gcactaaccg cggtaacctg agtgcccgga tgcaccaatg ccatctggct ctctggcact
      721 tctgcgttca cccagacggt attgagtccg ttgatgcgga ataacgttgc ccccgacgta
      781 acggtcatgc cctcccttgc tccgagttcc gcaatgacgc cgttgatggg agcgacgatg
      841 gttactgttg cctgtacttt tccggtggtc tccaccaggc gaatctggtc ttccgtcatg
      901 ccggcaagtc gcatgcgctg acgcgcgcct tctgtcaaat ccgtcaacac atcttgcagg
      961 ccgctcgccg agatgcgctt cgcggataga tattcttcct gcgccgcgac ccagtcgggc
     1021 acgtacagtt cggccaatgc ttgccctttg cgtactgcat cgagcggcgc gcgaacgtac
     1081 agcttttcca ggaagccatt gctgcgggct tgaacaacgg ctacgtctcg ctcgttgtag
     1141 gccaccgtac cgaccgcttg taccgagttc tcgagcgcgc cgcttatcac cgtagcggta
     1201 cgcacgccga ggttctgttg catgcgaggg ctaatggcga ccttgccttc gtccccttct
     1261 tcgccggcat acacgggcac cagtggcatg tccatgaagg gcgacttgcc gggcttgtcg
     1321 aatttctgac ccggcaccat cggatcgtgc cagtacagca cttttttatc cgagcctggt
     1381 cgtcctttat tcttcgcatc cggagcgggc tgattctcaa ccgtggccat tttcagtccg
     1441 cggttcatcc ccactacgta caacccatac cccgcaatgc tgaatgccgc aatggcggcg
     1501 atgaccagca caatcatccg tgagctcatt tgcttgctcc atcgttcgcg ttcgaaggtg
     1561 acgcaaaatc ggggaagagg aaattgagcc ccgcccacag ccgcgcggtc tcggcctcga
     1621 atcgcacctt ctgcatccga acgtcgattt cattgcgccg tgccagtaga agttccgtga
     1681 gggtactttt gccgccctgg tacgcggcgc gggctgcctt ggttcgctcc agcgccagag
     1741 gaagaagttc acgctcgtag cgcgcaaccc gctcacgccc gttctgccat tccgcaatca
     1801 ttgtgcgcac ttcgctaaca tgcgcacgca gcacttcttc gcgctcagcg cgcgcttgtt
     1861 cggccgccgc cagtttcgag gcaatctgtc tttcctggcg attgcgctga tcccattgca
     1921 acggaactga aacgccgatc gagaccatgt tcgaataggc aggcccgcgc aggctgtaac
     1981 tcagttcgac gctccagtcc gccttccgat ccgcctgcgc cagccgaact tcggttgctg
     2041 caatttgctc tcgttttgtg agaatggcga tctgtggatg gtgtccgagc tccatatcca
     2101 gcgtgctcga gtcaacgcga atagaatcca ttgccggctt ctcggagatc gaaaaagcag
     2161 gctcatcgcc tacccagcgc gccagcatgg tcctggcatt ggccacacgg cgccccactt
     2221 cactcgcctg atcttcaagc gtcgcccggg cgctgtgcgc ggcaaagaca tcggcctggc
     2281 ttcctcgtcc cgcccggtag gctgcttctg cagccgtgat ctctatcttg gcttgtgcgt
     2341 tttgttgcgc gatgactttg gcctgcgctt cagcgaaata gacatccagc caggcaagcg
     2401 cgctctcacg ctgaatcgac gcaatgacgg cttctttctc agcgagggat ttctcagctt
     2461 cgttctcaaa tcgttgtgcg cgcaattcac gtttgtcgga ccgcgtaaat tcctgcatga
     2521 caccgacgcg gcgcatggtc atgaagtcgt tggtcacact gaactgatcc ggcccgttga
     2581 ttggcaagtt gtctatgccg aaacgaagaa ccggatctgg taactgccca gccgcgacag
     2641 ccatttcgcg agacgcagtg acggaggcat tctgcgcggc tatttggcgc gagcgttcga
     2701 ccgctcggcg ctgagcttcc tgaatgctca acggcgcctc gtcggcgaga gcctcgcccg
     2761 gcaccagtac gagatccgcg cacaaggcca cgaggatagt ggcaaaaaaa attaacacgg
     2821 cgttgccgca gaactgggca acgatggttg gtagtgcggc ctttgaatat tggctgacgg
     2881 cagagcaggc ctgcgccagc gggaaagtag aagattgcat gatgcctccg caaagaaaac
     2941 acgaactgcc gcgtgcgcgg cgtattcagg tttccagcga aggctaaagt tgcagactac
     3001 aaaagcggat taacggcgga ggatcggttg tgggggagct tgcggcggtc aggacagcgc
     3061 cgggaacagc gcagtctgtg gcccagggca gcacgaggat tgcaacagaa gaggttgcga
     3121 ataccggcgt ttgcgcatga tccagacttt ggtcactggc gtttgcctgc tgcagacagc
     3181 tattcgggtt tacgcccttg tggcaatgat catcttgctt atcttgagaa aacgccatga
     3241 ttggcgacat ttcaggcgca acacagcttt gcgaggcatt cgctacctgc gcgaatacca
     3301 tcacgccaag tacaaatcga cacagcattg tgcctaagcg gcgaccacta ttacgcatgc
     3361 cgaaaattga agtcattcac gattgtgtca gtgtagccca agtgggcgca attttggggc
     3421 atgacggcag gaacacaact tcgttttgtg gcggaatgat tcagccctcc cccatatgag
     3481 tgacgatcat gagcggaatt gcgctcactg ccgcgactgt aatgattgcg aaatagagcc
     3541 aaccagccgc cgtgatgagg ggactgttgg catgcccctt cattaatttc ttgtcgcgcg
     3601 ccgttctcaa aatcgggtaa taggtaaagg gaagcaccac gactgcgaat acaacggagt
     3661 attcgactat caacagcggg ttgatgccgc tcatggcaat ggcaaagccg cttccgatca
     3721 acaccagcca ggtggcatgg aataccggcg cctgcttcat gttcttgtcc ttgccccacg
     3781 ccagcccgaa aaattgcgcc acgttataag caccggccag tgctgtttcg acggcggctc
     3841 cgccaatggc gaacagcatt cccagcagcg cgagcaaaag ccccttgatg ccaaaggccg
     3901 ccgtcgcggg caacgcggta gcactcagcg tatccggtat gacaccgtgc gagccaaaca
     3961 aggtcgcccc cgcgacaatc agggcagttg aaagcagacc acctagtgta aatcccactg
     4021 ttgctgttag aacgttggtt ggcagatcct tggccttcca tttgtcttcg atcgctccgg
     4081 aagaataaaa gtagatctca taaggcatga cgatcgaact gaacagaccc accacgtaat
     4141 agaaataaac caggacgttg tcctgtggca gttgcggcag gaatcccttg cccaccgcgc
     4201 tccagtcggg cttgagcgct acggcggcgg ccgcatagac cagcaatgcg agacccatca
     4261 acccgaatag ccgctccagc cattcaaact tcataaagta gactgcaata cagaggagtc
     4321 ccaccgccgt aatgagcatc agccggtatt ccagtccgaa cagcaactgc aggacgattg
     4381 caacgccgcc gatctcggcg gcacaggtca gaaggttgac gatgttcgaa gccaccaagg
     4441 tcatcaaacc gctgcggtag ctgacccgtt cgcggataat ctggaacgtg ggtttctgca
     4501 gcacggcggc aatcctgccc gccatttctc cgtaaaccat gatgccgatt gtgccgacca
     4561 ccacggtcca gatcagcatg taaccgaatt tggcgccgcc ctgtgtcgtg aagaccagtt
     4621 caccgatgtc aacgaatccg cccagtgccg acatgatgcc aagcgcgaga tcaagaatgg
     4681 aactcacagt gccgtttcca ggttaccgag ttgctgctcc agacgcatag cctgctgctt
     4741 gatcttcagg atctctggat tgtcggttcg gtgcctgctc aactcctgcc acattgcaga
     4801 caacgcttcc gcgctatttc gcacacgggt gccagcctct gccatttcgg gcggcaccgg
     4861 accgtcaagt tggcgcgccc tgcttcgtaa ttcctcctgc agtttttcgc aatgcacctt
     4921 ggcgtagtta tcagtgagcc ttccttgctc caattgcgtg ctgaacagca cgatctcgcc
     4981 ggcgatactc cccagtgcac gaacgtgtcg gccgaactct tccacgggca taggttcggg
     5041 ttcggtggat gcaatcttgg ccagcacgac ggctatcacc aggacagtag tggtgccgaa
     5101 aacaatcgca acccattttt tgatctggta cggcactgga atatcctgag cagcgaaagc
     5161 agattggata gaaccatccc gctattaatt tagatgtcat aacaacatgg aagttcggca
     5221 gaggctggga acagcaggcc gctgcgccat ggcacagcga acctaccagc tcgccggtgc
     5281 ggcgcatgat tccgcccacc gcacaccgga ccagtgagtt aatcgggacg tcgacggtgt
     5341 ggatcgccgt tgatctgcct gcgcgcaaac cagagcacca acggcggaat caccagccat
     5401 tgcagaatcg gcagcaatcc ggtgccgata aagggaagcc tcggcatagc caatgtgtag
     5461 ctccatcgtc ccgcaggtcc ggtcgccaca ccttcaacca gcgccgttgc aataattccg
     5521 atacccagaa atagggccag gtcctctttg cgtggcagca ggatccaatt gcggtttcgc
     5581 gctgcgatgg ccgtaatcca gaacgcgacc aggatgattg caacatctcc aagcgtcgtt
     5641 tgcgtgcaga tttttacccc caaccactgg ggctggtcag ccatgccgac gaagaacgga
     5701 atctgccaga actcccagac caggttcagc aaaaaggcaa atacgccgag attaaattcc
     5761 ggattttgta gaagtctgcg cggattcaag ataagtcaat cgatcagctc tgttgtttcc
     5821 ggatttgcat aatcgccact ggccgcactt cggtgccgcc gttggtgtca ttgtcaggaa
     5881 cattatcccg gcaaacagtc ggaacaccgg tgccggaact ctgccctatg aggatggtca
     5941 ctcagaaacc aaccccgaag gcttcctcac aattctccgg gagacatcat gaacgatcaa
     6001 atgcggtctt gcatcgacgc ctgctatgcc tgcgcgatag catgtgacga gtgtgcggta
     6061 tcttgcctga aggaagaaga ttttccgcat ctggcacgct gcatacagct tgatatggat
     6121 tgcgcggaca tgtgccgact tgcgatggcc tacatggggc gcgatagcga gctcatcaac
     6181 ctggtctgcc acacctgcgc tgaaatttgc gatgcctgcg cggaggaatg cgcgcatcac
     6241 aacaccatgg accattgcaa ggcctgcgaa gaggcatgcc ggcgatgcgc tcaggagtgt
     6301 cgcaagctaa tcgcc
//
LOCUS       DATMZQ010000040         3723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2086894, whole genome shotgun sequence.
ACCESSION   DATMZQ010000040 DATMZQ010000000
VERSION     DATMZQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086894"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="VN496_01395"
     CDS             <1..1656
                     /locus_tag="VN496_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15624.1"
                     /translation="AHYDFRHSLYREVLYGRLSEGARSKLHLLLAQRLKSFCDPCEQE
                     LATELALHFEGGRDYEQAVNYLLVAADNAVGRFAYRDCIEILHHAHELVLKVTLRVRA
                     ELEVRILEFIGHAHFALGALVDSAQTYAAAAARAQQAGLKAAQMHALTSAMYPLGFIG
                     PEEGVAALEEALKISMSVNDPPRLALTQMLAAGFRLVFDSWSETHAELCTSAYETLRC
                     LYPSELDPYQRIVYAHILMLKGKYAEALDVCERSVSEASSSGVGRVVNFLAHFGALSA
                     KIIILLRTGQLGKVLQITKAGRTSPDENLSLYWLLTLREAWLRTMAFDFEGARQICQA
                     TYNVRGEFPDGQYYAIDQIAAGNMALQQRRYAEALEHFKHVQDLDVHTKFFMHWAWRM
                     TAHLESINAWLLSGNIVNARTAAGGFLKSALSTSDPYLQALAWDLKARVAMAESDLQG
                     AREPIQRALAIVDSSEIQGAAWQIFATASQVCRHAKELKAAKTYRDRAESCILQIVDS
                     FEPDEPLRATFLTADPVRRILDGKIATKATRQHGLRHGAVPGA"
     gene            1942..3507
                     /locus_tag="VN496_01400"
     CDS             1942..3507
                     /locus_tag="VN496_01400"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXF15625.1"
                     /translation="MSLPLIQRAKEQSSHFVLTPKIAALSGGAPQCGTQGASQASLIP
                     QRSLLPGEQYRFHFDMTKCIGCKCCVVACNEQNGNPAEINWRRVGEIEGGWYPHTQRL
                     HLSMGCNHCIEPTCLAGCPVDAYSKDPVTGVVLHSAETCIGCQYCTWNCSYGVPQYNP
                     ERGVVGKCDMCHNRLAEGREPACVGACPEGAIQIEIVNIADWKQNYAASANAPGLPSA
                     DDSVSSTRVTLPETLPSNMQKADLNRVRSEDPHLPLVVMTVLTQLSVGTFATVWLLEL
                     LGKAKNLGMAAMISLLVGGLALGASTMHLGRPVLAYRALKMWRRSWLSREVLMFTCFS
                     GISGLYAALLWFRLPGSVVPGMLTSVIGLAGVTASACIYLVRARPAWNTKHTVADFHL
                     TGAYLGPLLVASLGVGNRSWLVPAAVTAAGLSILNQAIRFLWMTASDSFELQASAKLL
                     STVLASKVLARGVLLILGGIVVPLLLPTGFGLWTAMALALCGEILGRYLFFVSVVPKN
                     MAASYLSAGKVAA"
     gene            3504..>3723
                     /locus_tag="VN496_01405"
     CDS             3504..>3723
                     /locus_tag="VN496_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15626.1"
                     /translation="MNLKRQLGLDILREKYAFANDPVMGYTSDQRIPDKWVSTTCGYC
                     SVGCGLFIGVKDGRAVSVRGNCDHPVNVG"
BASE COUNT          791 a          997 c         1091 g          844 t
ORIGIN      
        1 gcgcactatg atttccgaca ttcactgtat cgggaagttc tctatgggcg gctgtccgag
       61 ggcgctcgtt cgaagctgca cctgttattg gcacaaagac ttaaatcctt ctgcgacccc
      121 tgcgagcagg aattggccac ggaacttgcc ttgcatttcg aaggaggccg cgactacgag
      181 caggccgtta attatttact cgttgcggcg gacaatgcgg tggggcgatt cgcctatcgt
      241 gattgcatcg agatattgca ccatgcgcac gaattggtgc taaaggttac tctcagagtt
      301 cgagccgaac ttgaggttcg catccttgag tttatcggcc atgctcattt cgcgctgggt
      361 gctctggtag attccgccca gacgtatgca gccgctgcgg cacgcgcgca gcaagctggc
      421 ctcaaagcgg ctcaaatgca cgcgctgacc tccgcaatgt atccattggg cttcattggt
      481 cccgaagagg gtgttgcggc actggaagag gccctgaaga tcagcatgag cgttaacgat
      541 cccccgcgtt tagcgctcac tcaaatgctg gccgcgggat tccgcctggt gtttgactct
      601 tggagcgaaa cacatgcgga actgtgtacg tccgcttacg aaactctgcg ctgtctgtat
      661 ccttcagagt tggatcccta tcagcgaatt gtatatgcgc atattttaat gcttaaaggc
      721 aagtacgcag aagcattaga tgtgtgtgaa cgcagcgtat ccgaagccag ctcatccggt
      781 gtaggtcggg tagtgaactt tctggcgcac tttggtgccc tgagcgcaaa aataatcatc
      841 cttctccgca ccggacaact tgggaaagtg ttgcagatta ccaaagccgg cagaacgtct
      901 cccgatgaga acttgtcact gtattggcta cttactctcc gcgaagcatg gcttcgaaca
      961 atggctttcg acttcgaggg tgcaaggcaa atttgtcaag caacctataa tgtgagggga
     1021 gagtttccag acggacaata ctacgcgatt gaccagatag ccgcgggaaa tatggcgctt
     1081 cagcaacgga gatacgctga agcgcttgaa catttcaagc acgtacagga ccttgatgtg
     1141 cacacaaaat ttttcatgca ttgggcgtgg agaatgacgg cgcatcttga atcgatcaat
     1201 gcctggctct tgtccggaaa tatcgtaaac gcacggactg cggctggcgg ttttctaaag
     1261 tccgcgctgt ccaccagcga tccgtatctg caggcgctag cgtgggatct gaaggcgaga
     1321 gtggcgatgg ccgaaagcga tttgcagggt gcgcgggaac cgattcagcg agcgctagca
     1381 atcgttgata gctccgaaat ccagggcgcg gcttggcaaa tatttgcgac tgcatcgcag
     1441 gtttgccggc acgcaaaaga actcaaagct gccaaaacgt accgcgatcg cgctgaatct
     1501 tgcatcctcc agattgtcga ctccttcgaa ccggacgaac cacttcgcgc aacattcctc
     1561 accgcggatc cagtccgccg gatcttggac ggaaaaatcg cgaccaaggc cacgcgacag
     1621 catggattga gacacggcgc ggtgcctgga gcttagcggt tgggtcgctt ccaccctggg
     1681 tgcgtcagcg actgtatgaa attggcgcct ggccctcaat ttagtcgatg agatgcaatt
     1741 gaattttgcg tttgcctgcg caccggcgat acgcgaacct cgaagcagat ttgtgacagg
     1801 gattcgcgcg tgattcgcat ccagagcaag cgaagaagcc aggtcccata ccttgaagtc
     1861 gaaagaaccg cggaatctta ggcggttcca cccatccgga taccgcgtcg tgtccgcagc
     1921 gaaagagcct gcgacgacac gatgtcattg ccccttatac agcgagcaaa ggaacaaagc
     1981 tcccacttcg tcctgacacc gaagattgcg gcactttcag gcggggcgcc gcagtgcggg
     2041 acgcaaggtg cttcacaggc gtcgttgatt ccccagcgca gtctcctgcc cggcgagcaa
     2101 taccgctttc atttcgatat gaccaaatgc atcggctgca agtgttgtgt tgtggcttgc
     2161 aacgagcaga acggcaatcc ggccgaaatt aactggaggc gggtcggcga aatcgaaggc
     2221 ggctggtatc cgcacacaca acggctgcat ttgtcgatgg gatgcaatca ctgcatcgag
     2281 cccacctgcc tggctggatg tcccgtggat gcgtattcga aggatcccgt cacgggcgta
     2341 gttctgcaca gtgccgaaac atgcatcggc tgccaatact gtacgtggaa ttgctcctat
     2401 ggcgtgccgc aatacaaccc agagcgcggc gtcgtcggaa aatgcgatat gtgccacaac
     2461 cggctggccg aaggtcgcga gccggcgtgc gtaggtgcct gcccagaggg cgccattcaa
     2521 attgagatcg tcaacattgc cgactggaaa cagaactatg ccgcaagcgc gaacgcgcct
     2581 ggactgcctt ctgccgatga cagcgtctct tcgacgcgag ttaccttgcc cgagacgttg
     2641 ccgtcgaaca tgcagaaggc ggacctaaat cgagtcaggt cagaagatcc gcacttgccg
     2701 ctcgtggtga tgaccgtgct gacgcagcta tcggtcggca cgtttgcgac ggtctggcta
     2761 cttgagttgt tgggaaaagc taagaacctc ggcatggcgg cgatgatttc gctgctcgtt
     2821 ggtggactgg cgttaggtgc atcaacgatg cacctcggac gtccggtgct cgcctaccgc
     2881 gctttgaaaa tgtggaggcg atcgtggctg agccgcgaag tcctgatgtt cacgtgcttt
     2941 tctggaatat cgggtctcta cgcagctctg ctctggttca ggctgccggg aagcgtcgtg
     3001 cctggaatgc tgacgtcggt catcggttta gccggggtaa ccgcgagtgc gtgcatttac
     3061 ctcgtgcgag ctcgcccggc atggaacacg aagcacaccg tggcagattt tcatctcact
     3121 ggtgcttacc tcggccctct tctagttgca agtctgggtg tcgggaaccg gtcatggcta
     3181 gtccccgcgg cggtcacggc tgcggggctg tcgatactca atcaagccat tcgatttttg
     3241 tggatgacgg cgtccgattc gtttgagttg caagcctcgg ccaagttgct ttcgaccgtg
     3301 ctcgcttcca aggttcttgc gcgaggcgtg ctgctgatcc tcggtggaat tgtggtgcca
     3361 ctgctgctgc caaccgggtt cggtctctgg actgccatgg ctctggcgct gtgcggagag
     3421 attctcgggc gctatctgtt ttttgtcagt gtagttccga aaaacatggc cgcttcctat
     3481 ctttccgccg ggaaagtggc ggcatgaatc tcaagcgcca actcggactg gatattttgc
     3541 gggagaagta tgccttcgcc aatgatccgg tcatgggcta cacctccgat caaagaattc
     3601 ccgacaaatg ggtttcgacc acgtgcggct attgctcggt gggctgcggg cttttcatcg
     3661 gagtgaaaga tgggcgggcc gtgagcgtcc ggggaaattg cgatcatccg gtgaacgttg
     3721 gaa
//
LOCUS       DATMZQ010000041         3185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2088050, whole genome shotgun sequence.
ACCESSION   DATMZQ010000041 DATMZQ010000000
VERSION     DATMZQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088050"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..2169
                     /gene="hyfB"
                     /locus_tag="VN496_01410"
     CDS             163..2169
                     /gene="hyfB"
                     /locus_tag="VN496_01410"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HXF15627.1"
                     /translation="MPVAVLLWCVLALLVIAVLSITRPKQPLATALVYAACAFTSVIA
                     FSAAFAALVSGQSGTLHLPLGIPWLGASFHVDALSAFFLVVVDFGAAAASVYALGYGR
                     NEHSPERVLPFFAAFLAGMNLVILADDAFTFLLSWEFMSLSSWALVLAHHRSADNVRA
                     GYVYILMASFGTFALLLGYGLLAGPDGNYGFEQIRSQHPDAFYSALVLGLALIGAGSK
                     AGLVPLHVWLPLAHPAAPSHASALMSGVMTKVAVYAFIRIVFDLLGPPVWWWGVLVLA
                     LGGITSLMGVLYALMQHDLKRLLAYHTVENIGIIFIGLGLALAFRANGLMATAALALT
                     AALFHVFNHSIFKSLLFFGAGAVLTATGERDMEHLGGLIHRMPFTAFAFLVGCAAISA
                     LPPFNGFVSEWMTFQAILLSPELPQWGLKLIVPAVGAMLALSAALAAACFVKAFGITF
                     LGRPRTGAAKHAKETDQFSLSVMLVFAAICLLVGVLPGFVIDAIAPAALVAIGGHMPL
                     QESVPWLSIVPIAEGRSSYNGLLVFVFIALSASLSAYVIHLLASRSTRRVPAWDGGFP
                     EASPLTQYTAGSFAQPIRRVFGTLVFRAREQVDFPPPGDPRPAKFQVNLEDVVWDVLY
                     APLSDTIAFVADRLNVLQFLTIRRYLSLVFAALVVLLLVIAAWR"
     gene            2160..3116
                     /locus_tag="VN496_01415"
     CDS             2160..3116
                     /locus_tag="VN496_01415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXF15628.1"
                     /translation="MALIRELTLQGSQMFLVVVIAPLITGVVRKIKARLLRRQGPPIV
                     QPYRDLIRLLRKEVVLAENASWLFRTAPYLIFSATWVAAALVPTFATGLMFSWTADLI
                     ALVALLGSARFFLALAGMDIGTSFGGIGSSREVMIASLAEPAMIMIVFSLALLAGTTQ
                     LSGIAQYTLSSNVGLRVTLGLAAISLIMVGIAENARIPVDNPATHLELTMVHEAMILE
                     YSGRHLALIELAASLKLLVYLSLIACIFLPWGIAPAGDSTMRFAIGAAAYAGKLLVGC
                     FALAVFETVIAKMRVFRVPTFLGAALMLGLLGALLLFVSRGL"
BASE COUNT          486 a          943 c          948 g          808 t
ORIGIN      
        1 ccaagctgat cacagctctt gaagccgccg gcgtggaact catccgggag aatgccccaa
       61 gctatggaag cgggcgcggc gtccggttga agatcgaagc ggacgctcga tccaggcgag
      121 caaaaaaaac tactccatca cgagcaactt agggacgacg cgatgccagt ggccgtcttg
      181 ctgtggtgtg tgctggcgct cctcgtgatt gccgtgctca gtatcacgag gccgaaacag
      241 cctcttgcga cggcgttggt ttatgcggca tgtgcattca cgtcggtgat cgcattttcg
      301 gccgctttcg cagcgctggt atcgggccag tccggaacgc ttcacctgcc actgggaata
      361 ccctggcttg gtgccagctt ccatgtcgat gcgctttccg ctttttttct tgtcgtggtc
      421 gatttcggcg ccgcggcggc cagcgtatac gccttggggt atggacgaaa cgagcactct
      481 cctgagcgcg tgttaccatt cttcgccgca tttcttgccg gcatgaacct cgtcattctg
      541 gcggacgacg cgtttacgtt cctgttgtcg tgggaattca tgtcgctgtc gtcttgggca
      601 ctggtgctgg cgcatcatcg ctccgccgac aatgtccgcg ccggctatgt ttacatcctt
      661 atggcgagct ttgggacctt cgcgctgttg ctgggctacg gtctgctggc gggccctgac
      721 ggcaattacg gctttgaaca aatccgatcg cagcatcccg acgcgttcta ttcggcactt
      781 gtccttggcc ttgccctgat cggtgccgga tcgaaggccg gactggtgcc attgcatgtg
      841 tggctgccgc tcgcgcatcc cgccgcacct agccatgctt cggctctgat gagcggagtg
      901 atgacaaagg ttgcggtcta cgcgttcatc cgcatcgtgt tcgacctgct agggccgccg
      961 gtctggtggt ggggcgtgct cgtgctggcg cttggcggca tcacatcgct aatgggcgtt
     1021 ctctacgcac tcatgcagca cgatctgaag cggttgcttg cctatcatac cgtagaaaac
     1081 atcggcatca ttttcatcgg tctaggtctt gcgcttgcct ttcgggccaa cgggttgatg
     1141 gcgacagcag cacttgctct gaccgcggcg ttgtttcatg tcttcaatca ctccatcttc
     1201 aagagcctgc tgttcttcgg cgcgggtgca gttctcactg ccaccggcga gcgcgacatg
     1261 gagcatctgg gcggcctgat ccatcggatg ccgttcactg cattcgcgtt tctggtcggc
     1321 tgcgcggcca tctcggcgct tccgcccttt aacgggttcg tgtcggagtg gatgacgttc
     1381 caggctattc tgctgagccc cgaactgccg caatggggcc tcaagctgat tgttcccgcg
     1441 gtgggcgcaa tgttggcgct ctccgccgcg ttggctgcgg cttgtttcgt caaggctttc
     1501 ggcatcacgt tcctcggccg tccccgcaca ggggccgcaa aacacgcgaa ggagactgac
     1561 caattttccc ttagcgtcat gttggtgttt gctgcgatct gtctgcttgt cggcgtcctg
     1621 ccggggttcg ttatcgatgc gatcgcgccc gcggcgctag tcgccatcgg gggtcacatg
     1681 ccgcttcagg agtcggttcc gtggctgtcg atcgtcccaa ttgcggaggg ccgcagttcc
     1741 tataacggac tgctggtgtt tgtgttcatc gcgctctcgg cctcgctcag cgcctatgtc
     1801 attcacctgc tcgcgtcgcg gtcgacgcgt cgggttccgg catgggacgg cggatttcct
     1861 gaagcgagtc cgctgacgca atacacggca ggaagttttg cgcagccaat tcgccgtgtc
     1921 ttcggaacgc tggtgtttcg cgcgcgcgaa caggttgatt ttccgccgcc cggagacccg
     1981 cgtcccgcaa aatttcaggt caatctggaa gatgtcgtct gggacgtttt atacgcaccg
     2041 ctctccgaca ccatcgcgtt tgtggctgat cgactcaatg tgctgcagtt tctgaccata
     2101 cggcgctatc tcagtctcgt ttttgccgcg ctcgtcgtgt tgcttctggt gattgcggca
     2161 tggcgctgat acgcgaactc accttgcagg gcagccagat gtttctggtc gtggtcattg
     2221 ctcctttaat caccggagtg gttcggaaga tcaaagcgag gctcctgcgt cgccaaggtc
     2281 cgccaatagt gcagccctat cgcgacctca ttcgcttgct gcgcaaagag gtggttctgg
     2341 ccgagaatgc gtcctggctg tttcgcactg cgccctacct gatcttttcc gcaacctggg
     2401 tggctgccgc tttggtgccg acctttgcca ccggtctgat gttcagctgg acagccgatc
     2461 ttatcgcgct ggttgccctg ctcggtagcg cgcgattctt cctggcgctg gccggaatgg
     2521 atataggcac gagttttggc ggcatcggct ccagccgcga agtcatgatc gcatcgctcg
     2581 ctgaaccggc catgatcatg attgtttttt ccctggccct actcgcgggc acgacacagt
     2641 tgtccggaat tgcgcaatac acgctctcgt ccaatgtcgg gctgcgcgtg acgctcggtc
     2701 tcgcggccat ttctctcatc atggtgggga ttgccgaaaa cgcacgcatc ccggtcgaca
     2761 atcccgccac acatctggaa ctcaccatgg tgcacgaggc gatgattctg gaatattccg
     2821 gacgtcatct ggcactgatc gaactggccg cttcgctgaa actgctggtc tatctttcgc
     2881 tcatcgcatg catctttctg ccttggggaa ttgcgcccgc cggtgattcc acgatgcgct
     2941 ttgcgattgg cgctgcggcc tatgccggca agctgctcgt gggttgcttt gcgttggcgg
     3001 tgtttgagac tgtgatcgct aaaatgcgcg tcttcagagt cccgactttc cttggagcag
     3061 cgctcatgct ggggctgctc ggcgctttgc tcctattcgt ttcgcgggga ctgtgaatgc
     3121 acggactggc atatgacgtc gcgcatcttc tggccggtag cctggtgctc gtcagcctga
     3181 tgatg
//
LOCUS       DATMZQ010000042        17819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1397517, whole genome shotgun sequence.
ACCESSION   DATMZQ010000042 DATMZQ010000000
VERSION     DATMZQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397517"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN496_01420"
     CDS             <1..185
                     /locus_tag="VN496_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HXF15629.1"
                     /translation="IEDDAFIGSDTQLIAPVTIGRGATIGAGSTITKDATADSLTLSR
                     VKQITISGWRRPVKKT"
     gene            235..2064
                     /gene="glmS"
                     /locus_tag="VN496_01425"
     CDS             235..2064
                     /gene="glmS"
                     /locus_tag="VN496_01425"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXF15630.1"
                     /translation="MCGIVGAVARRNIVPVLLEGLRKLEYRGYDSAGIAVVNGGLHRL
                     RSTGRVASLAEMTAQAAVQGDIGIAHTRWATHGVPSERNAHPHISEDGLAVVHNGIIE
                     NHDEIRARLLVGGYQFSSDTDTEVVAHLIHVYWKGDHDLFAAVRKAAAELQGAYAIAV
                     MSEKSPDMLVVARKGAPLLLGLGKGENFAASDASALLQVTKRMIYLEEGDVAEIGTDN
                     ICIRDSAGDEVVRPVHESQLSADAVELGPFSHYMQKEIFEQPGAVANTLEMVIGAHSL
                     VPQLFGVEAENILKRINAVTVLACGTSLHAGLVARYWIEALAGIPCSVEVASEFRYRK
                     SVPNPNALVIAISQSGETADTIAALQFAKALGQNSTLAICNVAESTLIRHASMRFLTR
                     AGPEIGVASTKAFTTQLASLFLLTLVLAKMHGRMPPEAEQKHLHALRHLPSALQQVLD
                     LEPMVAKWAERFADKRHALFLGRGMHYPIAMEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDKDMPVIAIAPNDTLLEKLKSNLQEVRARGGELYVFADDDTHIQAAEGVHVIRMT
                     AHAGDLSPILHTVPLQLLAYHAALTKGTDVDKPRNLAKSVTVE"
     gene            2107..2586
                     /locus_tag="VN496_01430"
     CDS             2107..2586
                     /locus_tag="VN496_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF15631.1"
                     /translation="MPKNSHAWFSSTIASAIFIVAVQSASGNMGAEESTESVDPNYVE
                     GKKALQSQDWKKAVDLLSKAVQAIPNDSNAHNLLGYAYRKTGNFDASFAQYKEALKLD
                     PSNKHAHEYIGEAYLLTGNLLIAEQHLADLQKICSPIPCEEYKELKLAVDAYKKSHK"
     gene            2604..3269
                     /locus_tag="VN496_01435"
     CDS             2604..3269
                     /locus_tag="VN496_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXF15632.1"
                     /translation="MANAAIRAVLWDFGGVMTESPFLAFRRFEREHGLPVDFLRGINA
                     QNHNSNAWARFERAELSPQQFDEAFAEETRAAGHEVRGLDVIDLLHGEIRPAMVTALK
                     ACKAHFINACVTNNVNAGPDRGLERDRSRAAEWRHVIDLFDFVIESSKIGERKPELRF
                     FQRVCEQLGIAASQAVYLDDIGGNLKPARIMGMHTIKVESPDAAIAELEAVLEIGLKQ
                     KHS"
     gene            complement(3266..4042)
                     /locus_tag="VN496_01440"
     CDS             complement(3266..4042)
                     /locus_tag="VN496_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15633.1"
                     /translation="MTGVAPKVVITGASSGIGKALAIEYARRGATLGLIARRNDRLRE
                     LELSLPVRPFIYTADVTDAHAMGDAAGDFVRRAGVPDIVIGNAGISAGTLTARIEDNA
                     VFEEIVRVNLTGLMLTFQPFIEAMRNRGSGSLVGIASVAGFRGLPGAAAYSASKSAAI
                     SYLESLRVEMRGSGVSVITICPGYVATPLTSKNPYRMPFLMDASDAARKIASAIHARK
                     RFYVFPWQMALVGWVLRRLPRPLYELLFAHAPHKPRRASG"
     gene            complement(4039..4590)
                     /locus_tag="VN496_01445"
     CDS             complement(4039..4590)
                     /locus_tag="VN496_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HXF15634.1"
                     /translation="MDYVPIPAQPVESRSKIEVIEFFYYGCESCNRLEPHLQSWLKTL
                     SPDVLFRRVPALRRTAWVPLTRVYFALEQLGEIDRMHSQIYQAVHDERINLGVSSELF
                     EWVQKKGIDRTKVEEVLDSDVVIAKVQQARDATLAYGVRATPSFVVDGRYLTSPGMLR
                     KSNALFPTLDFLIEKVRLTQLPK"
     gene            complement(4711..5370)
                     /locus_tag="VN496_01450"
     CDS             complement(4711..5370)
                     /locus_tag="VN496_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HXF15635.1"
                     /translation="MKFFRPLLITSLTVLGCLLTAGVTAKPVADKEYKLIDPPQAQPA
                     TVKGVEVLEFFNYACPHCFEFEPYLKSWLKAKPKNADFRYVPAVFNERMIPLAKMYYT
                     LEEMALLDKLHEKVYYAIHQQQLNLVDRAILLKWVGEQGVDTKKFEAVFDSFSVNNKV
                     QRATQMTRNYHIPGTPYLVIGGRYMTGPSMTVRADGNVDHNRLTQVVNELIDMASGGA
                     R"
     gene            complement(5405..5926)
                     /locus_tag="VN496_01455"
     CDS             complement(5405..5926)
                     /locus_tag="VN496_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXF15636.1"
                     /translation="MGLCIALGIALYINKGSNPFLQKQRAADKAAEKPAVDAPKQASA
                     PETPKPAAVATEKHAKAGETKPRFDFYKILPGTEEAVTDKEFKRTPPAAAKEVYFLQV
                     AAFQNPSDADNLKARLALSGIEAQIQTATLPDGQVWHRVRVGPFLNQDDIGKSRASLK
                     QNNLEANLIKVRQ"
     gene            complement(6029..7759)
                     /gene="argS"
                     /locus_tag="VN496_01460"
     CDS             complement(6029..7759)
                     /gene="argS"
                     /locus_tag="VN496_01460"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXF15637.1"
                     /translation="MSSDLRSHISELMHAALETVAPGALVAIVLERPKSSQHGDYASN
                     LAMQLAKPMKRNPRDIAQALIAAMPPSPLLQKAEIAGAGFVNLYLKPAAKQRIVQEVL
                     ENMSTFGRVKSKRATKVQVEFVSANPTGPLHVGHGRGAAYGASLSNLLEAAGFSVARE
                     YYVNDAGRQMDILALSVWLRYLETAGVRLPFPPNAYQGDYVREMGRLLHAAHKMRYVR
                     ASDAVLQDVPAADADPEAHLDGLIANAKKLLGSDYPSLHEFVLTEQLSDCRSDLLEFG
                     VTFDAWFSEKSLFDSGLVERATQRLEQSGHLYVKDGAKWFQSTEFGDEKDRVVQRENG
                     QYTYFASDIAYHINKYERGFDRMINVWGADHHGYISRVKGALKALALDPDRLTIALVQ
                     FAVLYRDGKKASMSTRAGEFVTLRELRNEVGNDAARFFYVLRKSDQHLDFDLDLAKSQ
                     SNDNPVYYIQYAHARVCSVMEQWGGDPAHLVNVDLTPLVEEQELQILLKLLDYPDQIQ
                     SAAHELAPHLVAFYLKDLAAGFHSYYNSTRLLVEDGVVREARLALAAAVRQVLRNGLE
                     ILGVGAPDKM"
     gene            complement(7814..9985)
                     /locus_tag="VN496_01465"
     CDS             complement(7814..9985)
                     /locus_tag="VN496_01465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXF15638.1"
                     /translation="MAIAKVALDCPLYSLFDYRFTGQAIQKGQLVIVPFRRRRTVGLV
                     FDVVERSQIEESRLRYIENILPVEPIPDTTLALIQFSSEYYQCPIGLAAFATLPAAMK
                     RVRYATPRQQWLYVLTEAGLALTPEQFPARATLKRRLCESFHAKGALRAPDLLDLSLG
                     GVRLAESWVKSGWIEKRPDVTTGTSDVAVTDQPTLTEEQRVAVATISATLGIFSPWLL
                     EGVTGSGKTEVYFRLIEQVAKRGLQTLLLVPEINLTPQLEERFRHRFPGLRLVALHSN
                     LSQQQRCANWLAARAGEVKLVVGTRLAIFTPLPRLGLIIVDEEQDSSFKQQEGLRYSA
                     RDLAIYCARQRQVPVVLGSATPSLETYQNARSGRFGYLRLSRRPVAAASDVRVVDIRG
                     MPGSHSFSEQVIKKMNACLVRGEQSLVFLNRRGFAPTLVCGACGWNAECGRCSARLVW
                     HVQHPGLHCHYCGYQEPLPSACPSCGNQDLKGLGQGTQRLERWLGELYPNARILRIDR
                     DSTRRKHAWQEMQADIRTELVDILVGTQMLAKGHDFPRLTLVVIVNPDSALFAADYRA
                     NEKVFQQLMQVSGRAGRADLPGEVLVQTQFPDHPLYQALIRQDYRSYVEELLKERKLA
                     GFPPFAYQALLRAESHSESILMNFLGHAAKHSISNSAVTLYDPVPATIAKIAGQYRGQ
                     LLAQSMVRAELQRFLPEWRSHLTSGKASRVRWSIDVDPIEL"
     gene            complement(10313..11374)
                     /gene="hemE"
                     /locus_tag="VN496_01470"
     CDS             complement(10313..11374)
                     /gene="hemE"
                     /locus_tag="VN496_01470"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HXF15639.1"
                     /translation="MSSTKNDVLLRALLREPTPYTPVWVMRQAGRYLPEYNLVRSRAG
                     SFLDLCRSPDLATEVTLQPLARFPLDAAILFSDILTIPDAMGLGLYFSEGEGPRFQRP
                     LREEREIRKLAAPDPGSELRYVLNAVTQIRAALAGSVPLIGFSGSPFTLACYMVEGCG
                     GTEFLELKKMLYERPELLHHMLDVNARAVADYLNAQIEAGVQAVMIFDTWGGMLTGEA
                     FHEFSLRYMQRVCGELRRERDGQKIPRIVFTKGGGHWLDSLAHCGADALSLDWTVDIG
                     KARARVGDRMALQGNLDPAVLLASPAVIRRETAKILTAYGHGSGHIFNLGHGISRLTP
                     PDNVAVLVDAVHSESARFH"
     gene            complement(11449..12879)
                     /locus_tag="VN496_01475"
     CDS             complement(11449..12879)
                     /locus_tag="VN496_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HXF15640.1"
                     /translation="MGKVTGFMEYQRLVEAAEDKRERVKHYREFVLRLADQEAGIQGA
                     RCMDCGIPFCMTGCPVNNIIPDWNDLVYRQDWKRALDVLHSTNNFPEFTGRVCPAPCE
                     AACTLNINNDPVGIKSIEHAIIDKAWEQGWVSPQPPKRKTGKRVAVIGSGPAGLACAQ
                     QLARAGHAVVLFEKNDRIGGLLRYGIPDFKMEKNSIDRRMEQMRAEGVEFQTNQNVGK
                     NVRAKDILAGFDAVALTGGAEQPRDLPVPGRDLDGIHFAMQFLPLQNKVVAGDQIPDQ
                     IVATGKHVVVIGGGDTGSDCVGTSNRQGALSVTQFELLPQPPAEEDKPLVWPYWPLKL
                     RTSSSHEEGCERDWAVATKEFVGENGKVRKIKIVRVQWKDGKMAELSHSETEIKADLV
                     LLAMGFLGPVQDGLIEQFAVETDARSNVKASTDGPKAYYTSVPKVFAAGDMRRGQSLV
                     VWAIREGRQCAHSVDSFLMGRSDLPR"
     gene            complement(12948..17597)
                     /locus_tag="VN496_01480"
     CDS             complement(12948..17597)
                     /locus_tag="VN496_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXF15641.1"
                     /translation="MAHSALPMQQGLYDPANEHDACGVGFIANIKGVRSHAIIEQGLQ
                     ILRNLTHRGAVGADPLAGDGAGILIQIPDQFFRDEMAIRGIKLPPSGEYGVGMVFLPQ
                     EPASRLACEYEIERAIKDEGQVLLGWRDVPRDNAGLGDSVKKIEPVIRQVFIGRGAGV
                     TVTDALERKLYIIRKVSGHAIQALKLEHSKEFYVPSMSARTLVYKGMLLAGQVGTYYK
                     DLCDPKVISALALVHQRFSTNTFPTWDLAHPFRMIAHNGEINTLRGNVNWLRARQGAI
                     FSPVLGKDLDKVWPLIYDGQSDSASFDNALELLIMSGYSLAHAMMMLIPEAWEKHSLM
                     DPHRRAFYEYHAAMMEPWDGPAAVAFSDGRQIGATLDRNGLRPARYLVTSDDVVVMAS
                     ETGVLPIPEEKIVKKWRLQPGKMFLIDLEAGRIIDDKELKDTLANTKPYRAWIERIRI
                     KLDDLPVSTKIAEPSKVALLDRQQAFGYTQEDQKFILAPMAEQGEEAIGSMGNDSPLA
                     VLSNKEKSLYNYFKQLFAQVTNPPIDPIREDLVMSLVSFIGPKPNLLGIDETNPPMRL
                     EVGQPVLDFNQMDAIRHIDYYTGGKFKSHEIDITYPVAWGKQAIEARLASLCAQAEDA
                     VRSGYSILIVSDRRIDAGHVAIPALLALSGIHQHLVGKGLRTSAGLVVETGSAREVHH
                     FALLAAYGAEAVHPCVAMETILHAEAPVPNEYRGEKGIKNYVKAIGKGLMKVMSKMGI
                     STYMSYTGAQIFEAVGLAKGMVDKYFTGTTSNVEGIGIFEVAEEAIRLHLAAFGDDPI
                     LVDALDAGGEYAFRVRGEEHMWTPDAIAKLQHSTRTNSIETFKDYARIINDQSKRHMT
                     LRGLFEFRVDPTKSIPIDQVEPAKEIVRRFSTGAMSLGSISTEAHTTLAVAMNRIGGK
                     SNTGEGGEDPRRYGIAKAGETLSSRLGRDRVQRDITLQEGDSLRSKIKQVASGRFGVT
                     AEYLNSADQLQIKMAQGAKPGEGGQLPGHKVSEYIAELRFSVPGVGLISPPPHHDIYS
                     IEDLAQLIHDLKNANPRASISVKLVSEDGVGTVAAGVSKAKADHVTIAGHDGGTGASP
                     WSSIKHAGTPWELGLAETQQTLVLNKLRGRIAVQVDGQMKTGRDVAIGAILGADEFGF
                     ATAPLVVSGCIMMRKCHLNTCPVGVATQDPELRKKFKGQPEHVVNFFFFVAEELRSIM
                     AALGIRRLSDMIGRTDLLDMKKGIDHWKAKGLDFSRIFRMPHMSPEVAIYNIEKQDHG
                     LEKALDHRLIELAQPAIQRGERVTIDMPIRNVNRTVGTMLSSEVARKYGNSGLPADTI
                     HVRFAGSAGQSFGAFIAQGITLDLIGEANDYVGKGLSGGRICVQPSPKFQGNPRENII
                     VGNTVMYGAVAGEAYFRGVAGERFCVRNSGAQAVVEGVGDHGCEYMTGGTVVVLGMTG
                     RNFAAGMSGGIAYVLDEENSFGTRCNLSMVHIEPVLSEVEQEAKIPRDIWHLGQADET
                     ILKAFIERHARYCNSVRAKEILGNWTVYRSKFVKVFPAEYRRALGEMAAKRRLAA"
BASE COUNT         4040 a         5030 c         4731 g         4018 t
ORIGIN      
        1 ttattgaaga cgacgccttc attggctcgg atacccagct gattgcgccg gtgacaattg
       61 gtcgcggtgc aaccattggc gcggggagca ccattaccaa agatgctacg gcagactcgc
      121 tgacattgtc gcgagtcaag cagataacga tctccggttg gcggcgtccg gtcaaaaaga
      181 cttagccgtt atgatctatc caatacaaca acaccccctt attgaacggt atctatgtgc
      241 ggaatcgtag gcgccgtagc taggcgaaat atagttccag tcttgctgga gggactacgg
      301 aagctggaat atcgcggtta cgattccgcg ggtatcgcgg tggttaatgg tggtctgcat
      361 cgcttgcgca gtacgggtcg cgttgcttcg cttgccgaaa tgacagcaca ggccgcagtg
      421 caaggcgata tcggaatcgc tcacactcgc tgggccactc atggcgtgcc ttcggaacga
      481 aacgcccatc cgcacatcag cgaggatgga ctcgccgtgg ttcataacgg catcatcgaa
      541 aaccacgatg aaatacgggc tcggctactg gtgggtggct accagttttc ttccgatacg
      601 gacactgaag tagtcgcaca tctgattcac gtctattgga aaggcgatca tgacttgttc
      661 gccgcagttc gcaaggccgc tgcggaactg cagggcgctt atgcaatcgc agtaatgtcg
      721 gagaaaagcc cggatatgct tgtggtggcg cgtaaaggcg cgccgctgct gctggggctt
      781 ggcaaaggag aaaattttgc cgcttcggac gcgtctgcac tgctgcaggt aaccaagcga
      841 atgatctatc tcgaagaggg tgacgtagcg gaaattggca ccgacaacat ttgcattcgc
      901 gatagtgccg gggatgaagt tgtgaggccg gtgcatgaga gccaattatc ggcagatgcg
      961 gtagaactcg ggccgttcag tcattacatg cagaaggaaa tcttcgagca gcccggggca
     1021 gtggctaaca cgctggaaat ggtcattggc gcccactcgc tggtgccgca gttgtttggt
     1081 gttgaggcgg aaaacatcct gaagcgcatc aacgcggtga ccgtactggc ttgcggaacc
     1141 agtttgcacg ccggtctggt ggcccggtac tggatcgagg cactcgccgg cataccttgc
     1201 tcagtggaag ttgccagtga attccgatat aggaaaagcg ttccgaatcc gaatgcgctc
     1261 gttattgcga tttcacaatc cggagaaacc gccgatacca tagccgcgct gcagtttgcc
     1321 aaagcgctgg ggcaaaattc cacgttggcc atctgtaacg tcgcagagag tacgctgatc
     1381 cggcacgcga gtatgcggtt cctaacgcgg gcgggcccgg aaatcggcgt cgcttcgacc
     1441 aaggcattta ccacacaact cgcctcgttg tttttgctga ctctggttct cgcaaaaatg
     1501 cacggccgca tgccgccgga agcggagcaa aagcatcttc acgcgctacg ccacttgcct
     1561 tcggccttgc aacaggtact tgatctcgaa ccgatggttg cgaaatgggc ggagcgtttt
     1621 gccgacaagc gccatgcgct ttttctcggt cgaggcatgc attatccgat cgccatggaa
     1681 ggcgcgctca aattgaaaga aatttcctat attcatgctg aagcctatcc ggccggggaa
     1741 ttgaaacacg gccccctcgc gctggtggac aaggacatgc cggtaatcgc aattgcgccc
     1801 aacgacacgc ttctggagaa actcaaatcc aatcttcagg aagttcgcgc gcgtggcggc
     1861 gaattgtatg tgttcgcgga cgacgatacg cacatacagg cagcagaagg cgtacacgtc
     1921 attcgcatga ctgcccatgc gggcgactta agtccaattc tccatacggt gccgttgcaa
     1981 ctgctcgcgt atcacgccgc cttaacaaag ggtacagacg tcgataagcc gcgcaatctg
     2041 gcaaaatccg tcactgtaga atagcagggc ggtcagcata cggccgacgg actccacgga
     2101 ggtatcgtgc ctaaaaattc tcatgcttgg ttttcttcaa ctatcgcctc ggcgatcttt
     2161 attgttgccg tgcaatcggc ctccggaaac atgggtgccg aggaatccac cgaaagcgtg
     2221 gatccaaact acgtggaagg caagaaagct ttgcaatcgc aagattggaa aaaagcggtg
     2281 gatttgctgt caaaggcagt tcaggccata ccgaacgatt ccaacgccca caaccttctg
     2341 ggttatgcgt accgcaagac cggcaacttc gatgcctcat tcgcgcagta caaggaagcg
     2401 ctaaagctcg atccaagcaa caagcacgcg catgaataca ttggtgaggc ttacctgctt
     2461 acgggaaact tgttaattgc ggagcaacat cttgccgatt tgcagaaaat ttgttcgccc
     2521 attccatgcg aggagtacaa agaactcaaa ctggccgtcg acgcttataa gaaatcccat
     2581 aaatagccag ccatagatcc aacatggcaa acgcggcgat tcgtgcggtg ctatgggatt
     2641 tcggcggcgt tatgacagag agtccgtttt tggctttccg ccgtttcgag cgcgagcacg
     2701 gtttaccagt cgactttctt cgtgggataa acgctcaaaa ccacaacagc aatgcttggg
     2761 cgcgcttcga gcgcgctgag ctgtcaccgc agcaattcga cgaggcattt gccgaggaga
     2821 cacgcgcggc cggtcatgaa gtgcgaggcc tcgacgtcat tgatttgctc catggcgaaa
     2881 tccggcctgc aatggtgacg gctctcaagg cctgcaaggc gcacttcatt aatgcatgcg
     2941 tcaccaacaa cgttaatgca gggccggatc gcgggctaga acgtgatcgc agtcgcgcag
     3001 ctgaatggcg gcacgtcata gatttatttg acttcgtcat cgaatcttca aaaatcggag
     3061 aacgtaagcc cgagttgcga tttttccagc gggtttgcga acaacttggc atagctgcgt
     3121 cccaggcggt ttacctggat gatatcggtg gcaatttgaa gcccgcacgg ataatgggaa
     3181 tgcacaccat caaagtcgag agcccagatg cagcaatcgc cgaactcgag gccgtattgg
     3241 aaatcgggtt aaaacaaaaa cacagttagc cagaggcgcg acggggcttg tgcggcgcat
     3301 gtgcgaacaa cagttcgtac aatggccgcg gcagtctgcg caacacccaa cccaccaggg
     3361 ccatctgcca gggaaagacg taaaaccgct tgcgtgcatg aattgccgat gcgatttttc
     3421 gggcggcatc ggaagcatcc atcaagaacg gcatgcggta aggatttttc gacgtaaggg
     3481 gagtagcgac atatcccggg caaatcgtta tgaccgatac gccactgcca cgcatttcga
     3541 cgcgaaggct ttccaggtaa gaaatcgcgg ccgacttcga tgccgaatat gccgctgctc
     3601 ctggcaaacc gcgaaagccc gccacacttg caattccgac cagactaccg cttccccggt
     3661 tgcgcattgc ttcgatgaac ggttgaaaag tcaacatcaa tccagtcaag ttcactcgca
     3721 ctatttcttc gaacaccgcg ttatcttcaa tccgggcggt gagtgtgccc gcgctgatgc
     3781 ccgcattccc gataacaata tcagggactc ccgcgcggcg gacaaaatct ccagcggcat
     3841 cgcccatggc gtgcgcatca gttacatcgg ccgtataaat gaaggggcga accggcaggg
     3901 ataactcaag ttccctaagc cggtcattac gccgcgcgat gagcccaagc gttgccccgc
     3961 gccgtgcata ttcgattgcc agcgccttac ctatgccact ggaggcaccc gtgatcacga
     4021 ctttgggcgc gactccggtc attttggaag ttgcgttaaa cgaaccttct cgataagaaa
     4081 atccagtgta ggaaaaagcg cattggactt tcttagcatc cctggactcg tcaagtatct
     4141 gccgtcgacg acgaaagaag gcgtggcgcg tacgccatac gccagggtcg cgtctcttgc
     4201 ctgttgcact tttgcgatta cgacgtcgga atccaggact tcctctacct tggttcgatc
     4261 aatccctttt ttttgcaccc attcgaaaag ttctgaagat actccaaggt taatgcgctc
     4321 atcgtggacg gcctgataga tctgcgagtg cattctgtcg atttcgccaa gttgttccaa
     4381 agcgaaatag accctggtta gcggcaccca tgccgtgcgc cgcaacgccg gaacacggcg
     4441 aaacaaaacg tctggagata acgtcttcag ccaggactgc agatggggtt cgaggcgatt
     4501 gcaagactcg caaccataat agaagaattc aattacttcg attttgctcc tggactctac
     4561 cggttgggca gggattggta cgtaatccac gccttccaac ggttccgcaa ttgcgaaacc
     4621 cccaacccaa cccgaaacaa taatcgttgt taacagcctt agcgggcgcg ataggacatc
     4681 gctaaaccgc ttcatggtca acagaaacgg tcaacgcgct ccgccggagg ccatatcgat
     4741 gagttcattc acgacttgcg tgagtcgatt atggtcgacg ttgccgtcgg cgcgcactgt
     4801 catggaggga ccagtcatgt accgaccccc gataacgaga taaggggtgc ccggaatatg
     4861 ataattccga gtcatctgcg tagcgcgttg cactttattg ttaacgctaa aggaatcaaa
     4921 caccgcttca aattttttgg tatccacccc ctgctcgcca acccacttca acaggattgc
     4981 ccgatcaacc agattcaatt gttgctggtg gattgcgtaa taaacctttt cgtgcaattt
     5041 atccaacaaa gccatttcct caagcgtgta gtacattttc gctaggggaa tcatgcgttc
     5101 attgaaaact gccggaacgt agcggaagtc cgcgtttttt ggcttcgcct tcagccatga
     5161 tttcaaatac ggctcgaatt caaagcaatg cgggcaagcg tagttgaaga actccagtac
     5221 ctccacgcct ttgacagtgg ccggttgcgc ctgcggagga tctatcagct tgtattcctt
     5281 gtccgcaacg ggcttggcag tgactccggc agtcagcaag catcccaaga cggttagcga
     5341 cgtgataagt aaagggcgaa aaaatttcat ttgtctttcc tgtgacatta ttggttcatc
     5401 agcgctactg tcgcaccttg atgagattcg cttccagatt attttgcttc agcgaagcgc
     5461 gtgacttgcc gatgtcatcc tgatttaaga aaggaccaac ccgcacacgg tgccacacct
     5521 gtccatccgg caatgtagcg gtttggattt gcgcttcaat gccggagaga gccaaacggg
     5581 ccttgaggtt atccgcatcg gaagggttct gaaacgccgc aacttgcaaa aaatagactt
     5641 ctttggcggc ggcaggtggc gttcttttga attccttatc cgtaaccgct tcttcggtac
     5701 ctgggagaat cttgtagaaa tcaaaacgtg gctttgtctc gcccgctttg gcatgttttt
     5761 cagtcgcgac tgcggctggt ttgggtgtct cgggagcgga tgcttgtttt ggcgcatcga
     5821 ccgcgggctt ctcggccgcc ttgtcggcag ctctttgctt ttgcagaaag ggatttgaac
     5881 ccttattgat ataaagcgct atcccaagcg caatacaaag acccaccagc aaaccgatca
     5941 gaatgccaat ccacatcgga ctgccacgtc caccggttgc ggcagttccg ctgtttttgt
     6001 aatcgcggct cataagtgga agtttacctt acatcttgtc gggcgcgccg acacccagga
     6061 tttcaagccc gttgcgcaat acctggcgaa ccgctgcagc aagcgcgagt cgcgcctccc
     6121 tgacgacgcc gtcttccacc agaagacgcg tagagttgta gtagctgtga aagccggccg
     6181 caagatcctt gagatagaac gccaccaagt gcggcgcaag ttcatgggcc gcgctttgta
     6241 tctggtccgg gtaatcgagc aatttcagca aaatctggag ctcctgctct tcaacgagcg
     6301 gggttaagtc cacgttgacg agatgtgctg gatcgccgcc ccattgctcc atgactgagc
     6361 aaacccgagc atgcgcgtac tgaatgtagt acacgggatt gtcattgctt tgtgactttg
     6421 ccagatccag atcgaaatcc agatgctgat cgcttttgcg cagtacatag aagaagcgcg
     6481 ccgcatcgtt gccgacctcg ttacgtaatt cgcgcagggt gacgaattca ccggcgcgag
     6541 ttgacattga tgccttcttg ccatctcggt acagcacagc gaactgcacc agcgcgatgg
     6601 tgagccggtc agggtccaaa gccagtgcct tcaatgcgcc tttaacccgc gaaatgtagc
     6661 catggtgatc cgcgccccag acattgatca tgcgatcaaa cccacgctca tatttattga
     6721 tgtgatacgc aatgtctgaa gcgaaatagg tgtattgacc gttctcccgc tggacgacgc
     6781 ggtctttctc gtcgccaaac tccgtcgatt ggaaccactt cgctccatct ttaacataca
     6841 gatgtccgct ttgttccagc ctctgcgttg cacgctccac gagtccagag tcgaacaatg
     6901 atttttcgga gaaccaggcg tcgaaggtca ctccaaattc gagcaagtca cttcggcaat
     6961 cagaaagttg ctctgtcagc acgaattcat gcaggcttgg ataatcactg ccaagcagct
     7021 ttttggcgtt agcgatcaac ccatcgagat gtgcttccgg atcggcatcc gcagccggca
     7081 cgtcctgcag caccgcatcg gacgcgcgta catagcgcat cttatgcgcg gcgtgcagca
     7141 gccgccccat ttcccgcacg taatcgccct ggtaagcatt cggcggaaat ggcaaccgca
     7201 caccggccgt ttcaagatag cgtagccaca ctgacaacgc caagatgtcc atctgccggc
     7261 cggcgtcatt gacataatac tcacgtgcga ccgaaaatcc ggcggcttcc agcagattcg
     7321 aaagacttgc tccgtaggcg gcacctcgtc cgtggccgac atgtaaaggt ccggttggat
     7381 tggctgaaac aaactctact tgcacctttg ttgcccgttt tgatttaacg cgtccaaacg
     7441 tgctcatgtt ttcgagcacc tcttgcacga ttctttgctt cgcggcgggc ttcagataca
     7501 aattaacaaa cccggcaccc gctatttccg ctttctggag caacggtgat ggaggcattg
     7561 ccgcaatcag cgcttgtgcg atatcgcgcg gattgcgctt catgggcttc gcgagctgca
     7621 tcgccaaatt ggacgcgtag tcgccgtgct gggaagattt gggacgttcg agcacaattg
     7681 caacgagcgc acccggtgcg acggtctcga gcgcagcatg cattaactcg cttatatggg
     7741 agcgcaaatc tgacgacatt tggacacggg tgcgaagaat ccggaattat accggctcga
     7801 tctcaggcga acgtcagagt tcaataggat cgacgtcaat ggaccagcgc acacggctgg
     7861 ccttgccact cgtcagatgt gagcgccact cgggcaggaa gcgttgcaat tccgcacgca
     7921 ccattgattg tgcgagcagt tgaccacgat attgtccggc tattttggcg atcgtggccg
     7981 gaaccggatc ataaagcgtg acagcagaat tgctgattga atgcttcgct gcgtgaccga
     8041 ggaaattcat caatatcgat tcgctgtgag attcggcacg caggagcgcc tgatatgcga
     8101 acggcggaaa gcccgcgagc ttgcgctcct tgagcagctc ttcaacgtag ctccgatagt
     8161 cctggcgtat cagcgcttga tacaacggat ggtcgggaaa ttgcgtttgg actaacacct
     8221 caccgggcag atcggctcgc ccggcacgtc cagacacctg catgagttgc tggaatactt
     8281 tttcgtttgc ccgatagtcc gcggcgaaca acgcactgtc tggattaaca atcacgacca
     8341 acgtgagtct gggaaagtca tgcccttttg cgagcatctg cgtaccgacc aggatatcta
     8401 caagttccgt gcgtatatcg gcctgcatct cttgccatgc atgttttcgt cgagtgctgt
     8461 cgcgatcgat gcgcaggatc cgcgcattgg gatagagttc tcccagccat cgctccaggc
     8521 gctgcgttcc ttgccccaaa cccttcagat cctgatttcc acatgaaggg caggcactgg
     8581 gtaaaggctc ctggtatccg caatagtggc agtgcaaacc tgggtgttgc acgtgccaga
     8641 ccaggcgcgc gctgcagcgc ccgcattcgg cattccaccc gcaggctccg cacaccaagg
     8701 tgggcgcgaa tccccggcga ttgagaaaga caaggctttg ctcgccgcgg acaaggcaag
     8761 cgttcatctt ctttatgact tgctcactaa aactgtgcga tccgggcatg ccgcgaatat
     8821 caacaacgcg gacatcagat gctgcagcga ctgggcggcg gctgaggcgc aagtagccga
     8881 agcggccact acgagcgttt tgataggtct cgagagacgg cgttgccgag ccgagcacaa
     8941 ccggcacctg gcgttgtcgg gcgcaataga tcgccagatc ccgcgccgaa taacgcaatc
     9001 cctcctgttg tttgaaggag ctatcctgct cctcgtcgac gatgatcaag ccgagccgcg
     9061 gtagcggggt gaagatggcc aagcgtgtcc cgactactag ctttacttcg ccggcgcgtg
     9121 ccgcgagcca gttcgcacac cgctgttgct gggaaagatt gctatgtaat gcaaccagcc
     9181 gcaatcctgg aaaacggtgc cgaaatcgtt cctccagttg cggcgtgagg tttatctctg
     9241 gcaccaataa cagcgtttgc aagccgcgct tggcaacttg ttcaataagt ctgaagtaaa
     9301 cttcagtctt cccgctcccc gtcacgccct ccagcagcca aggggaaaaa attccgagag
     9361 tagccgaaat cgtcgcaacg gcgacgcgct gttcctcggt tagcgtgggc tgatcagtga
     9421 ccgcaacgtc agaagttccg gttgtgacat cgggacgttt ttcaatccac ccgcttttta
     9481 cccacgactc ggctagcctt acaccgccga gcgacaagtc caacaaatca ggcgcacgaa
     9541 gagctccctt tgcatggaat gattcgcaca atcgacgctt cagggttgcg cgcgcgggaa
     9601 attgttcagg agtcagggcg agtccggctt cagtaagcac atacagccat tgctgccttg
     9661 gtgtagcgta ccgaacacgc ttcatggcag ccggcaatgt cgcgaaagcc gccagaccta
     9721 ttggacactg atagtactcg ctcgaaaact ggattaacgc gagagtagtg tccggtatcg
     9781 gctccaccgg caagatgttt tcgatgtagc gaaggcgaga ttcctcaatt tgcgatcgct
     9841 ccacgacgtc gaacaccaag cccaccgtgc ggcgcctccg gaaggggaca atgacaagct
     9901 gacctttttg aatcgcttgt ccggtaaagc gataatcgaa caaagaatat agtgggcagt
     9961 ccagcgcaac ttttgcgata gccatgtcag atagccgaaa tatgcggcaa cattattggc
    10021 ttgccccaaa gagtagtcgg gtgcgctaaa tgactttccg aaaagacctt tttggaaggg
    10081 cccttgtgac tgtggataat tctgttgatt aaacctaaca ctcgccctct gggaaatccg
    10141 tctgaaaccc agacaggatg cgggatttcg cgtgtcaatc taaattacgc gtccagattc
    10201 agctagttac agaattctct gtaaagaacc gcttgctcgc cgggtttttg ttctgaacgc
    10261 gcccaaaatt gtgcataagc gcatacgcag gacgctaaat tggggctcca ggtcagtgaa
    10321 atcgagcgct ctccgagtgt accgcgtcca ccagcactgc aacgttatca ggaggcgtaa
    10381 gccgcgaaat cccatggccc aaattgaata tatgtccact accgtgccca tacgccgtca
    10441 ggatcttggc tgtctcccgg cgaatgacag ctgggcttgc tagcaacact gccgggtcaa
    10501 gattcccttg cagcgccatt cgatcaccga cccgggcgcg cgccttgcca atatcaaccg
    10561 tccagtccaa gctcaacgcg tccgcgccgc aatgcgccag gctgtctagc cagtgcccgc
    10621 ctcctttggt aaacacaatg cgaggtatct tttgaccatc ccgctcccgt cttaactctc
    10681 cgcacactcg ctgcatgtag cgcaaggaaa attcgtgaaa tgcctctcct gtgagcatgc
    10741 ctccccaagt gtcgaaaatc attactgcct gcacaccagc ttcaatctgc gcgtttaggt
    10801 agtccgcaac ggcgcgcgca ttaacgtcga gcatgtgatg caaaagttcg ggacgctcat
    10861 agagcatctt cttcaattcc aaaaactcgg tgcccccgca gccttccacc atatagcaag
    10921 ccagggtaaa cggactacca gagaagccta tcaggggaac ggatcctgcc aatgcagcac
    10981 gtatctgagt aactgcgttc aggacgtacc tgagttcaga acctggatct ggtgccgcca
    11041 acttgcggat ttcgcgctcc tcccgcaagg ggcgctgaaa gcgcggccct tcgccttccg
    11101 aaaagtagag gcccagaccc atcgcatccg ggatggttaa aatatcggaa aaaagaatgg
    11161 cggcgtccag cggaaaacgt gccagaggtt ggagggtaac ttcggtagct aagtctggac
    11221 tcctgcacaa atccaggaaa ctgccagcgc gcgaacgcac taggttgtat tccggaaggt
    11281 agcgacccgc ctgccgcatg acccataccg gcgtgtatgg cgtaggctct cgcaaaagag
    11341 cgcgcaacag aacgtcgttc ttggttgagg acacgtcttg aagttggctt gatcgctaca
    11401 caggcgcctg tgctgctcag ggcgcttcgt tgttaagcgc ttgttatatc aacgcggcag
    11461 atcggagcgg cccatcagaa agctgtccac ggagtgagcg cactgccggc cttcacgaat
    11521 ggcccacacc acgagcgatt gaccgcggcg catgtcacct gcagcaaata ctttgggaac
    11581 ggacgtgtag tacgcttttg gcccgtcggt cgaggctttt acgttgctgc gggcatctgt
    11641 ttcaacagca aactgttcaa tcaatccatc ctggaccggg cccaagaatc ccattgcaag
    11701 gagcaccaaa tccgctttta tctcggtttc cgagtgggat agttcagcca ttttgccgtc
    11761 cttccactgc accctcacga ttttgatttt cctgactttg ccgttttcgc cgacgaactc
    11821 cttggtagcg acagcccaat cgcgttcaca tccttcttcg tgcgacgagg aggtccttag
    11881 cttgagtggc cagtaaggcc acaccagcgg cttgtcttct tctgccggcg gttgaggcag
    11941 caattcaaac tgggtaacag acaaggcacc ttgtctgttc gatgtaccga cgcaatccga
    12001 accggtatcg ccgccgccaa tcacgactac atgcttgccg gtagctacga tctgatcggg
    12061 aatctggtcc ccggctacca ctttattctg caggggcaag aactgcatgg cgaaatgaat
    12121 gccgtcgaga tcgcgacccg gaaccggcag gtctcggggt tgctctgcac cgccggtgag
    12181 tgccactgcg tcgaacccgg ccagaatgtc cttcgcgcgc acattcttcc ccacattttg
    12241 gtttgtctgg aattcaaccc cttcggctcg catctgctcc atgcggcgat cgatagaatt
    12301 tttttccatt ttgaagtcgg gaataccgta gcgcagcagg ccgccaatcc ggtcattctt
    12361 ctcgaataga acaacagcat gtccggcacg tgcaagctgc tgcgcgcatg ccaggccggc
    12421 agggccggac ccgatcaccg cgactctctt ccccgtcttg cgcttgggcg gttgcggcga
    12481 cacccatcct tgttcccacg ccttgtcgat gattgcgtgc tcgatggatt tgattccgac
    12541 cggatcgtta ttgatgttga gcgtgcaggc ggcctcgcaa ggagcggggc aaacccgtcc
    12601 ggtgaattcc ggaaaattat tggtggagtg caaaacatcg agtgcccgtt tccagtcttg
    12661 tctatacaca agatcattcc agtccgggat gatattgttt accgggcagc cggtcatgca
    12721 gaaaggaatg ccgcaatcca tgcagcgcgc gccctgtatg ccggcttcct ggtcggcaag
    12781 gcgaagcacg aattcacgat agtgctttac gcgctcgcgc ttgtcctcag ccgcctctac
    12841 aaggcgctgg tactccatga aaccggtgac ctttcccatt accgccttcc aattatgttc
    12901 agggccgcga gaatcgcggc cctgccgcca tcagtaattt tttggagcta tgcagccaga
    12961 cgacgttttg cggccatctc accgagcgca cgccggtatt ctgccggaaa tactttgacg
    13021 aatttggaac gataaactgt ccaattgccc aatatttctt tcgctcgaac actgttgcag
    13081 taacgcgcat gcctttcgat gaacgctttg agaattgtct cgtcggcttg ccccaagtgc
    13141 caaatatccc gtgggatttt tgcttcctgc tccacttcgg aaaggacggg ctcgatatgg
    13201 accatcgata gattgcaacg agtgccgaaa ctgttttcct cgtccagcac gtatgcgatt
    13261 ccacctgaca ttccggctgc aaagttgcgt cccgtcattc cgagcacaac cactgtgccg
    13321 ccagtcatgt actcgcaacc gtggtctcca acaccttcga ccacggcctg tgctcccgaa
    13381 ttccgaacgc aaaagcgctc ccccgccaca ccgcggaaat atgcttcgcc ggcgactgct
    13441 ccatacatta cggtattgcc tacgatgatg ttttccctcg gattgccctg gaactttggg
    13501 gagggctgta cgcagatgcg gccaccggac aagcccttgc cgacgtagtc gttcgcctca
    13561 ccgattaaat ccagcgttat gccctgcgcg ataaaagctc cgaagctctg ccctgcgctg
    13621 cctgcgaatc tcacatggat ggtatcggcg ggtaagccgg aattgccata cttccgagcc
    13681 acctcagacg aaagcatcgt accgacagta cggttcacat tacgaattgg catatcaatc
    13741 gtcactcttt cgccacgctg aatggctggc tgggccaatt cgatgagccg gtggtcaagg
    13801 gccttctcga gcccatgatc ctgcttttca atattgtaga tcgccacttc aggcgacatg
    13861 tgcggcatcc tgaagatacg ggagaaatcc aaacctttag ccttccagtg atcaataccc
    13921 tttttcatgt ccagcaagtc ggtgcggccg atcatgtcgc tcaatctgcg tatgccgagc
    13981 gctgccatga tggaacgcag ttcctctgcg acgaagaaaa agaagttcac cacgtgctcg
    14041 ggttgtcctt tgaacttctt gcgtagctcg ggatcctgtg ttgctacgcc gaccggacaa
    14101 gtgttgagat ggcacttgcg catcatgatg cagccactca ccaccagcgg ggcagtagcg
    14161 aagccaaact cgtccgcccc cagaatcgcg ccgatggcga catcacgtcc ggtcttcatc
    14221 tggccgtcca cctgcaccgc tatacgaccg cggagcttgt ttaacacgag tgtctgctgg
    14281 gtttcggcca gacccagttc ccatggcgta ccggcgtgct tgatggagga ccagggcgaa
    14341 gcgccggtac cgccatcatg ccccgctata gtgacgtgat cggctttcgc ctttgataca
    14401 ccggccgcaa cagtgccgac accatcctct gataccagct tgacactgat cgatgcacgc
    14461 ggattggcgt tcttcagatc atggatcaac tgcgccagat cctcgatcga atagatgtcg
    14521 tgatggggcg gcggcgaaat cagccccacg ccaggcaccg aaaagcgcaa ttccgctatg
    14581 tattcggaga ccttgtgtcc aggcagctgg ccaccttcgc cgggcttggc gccttgtgcc
    14641 atttttatct gaagttgatc ggcactattc agatattcgg cagtcactcc gaaacgaccg
    14701 gaagcgacct gcttgatttt ggaacgtagc gaatcgcctt cctgcaatgt gatatcacgt
    14761 tggacgcgat cgcgccccag acgactgctt aacgtttcgc ccgcttttgc gatgccatac
    14821 cggcgcgggt cttcaccacc ttccccggta ttcgatttgc ccccgatgcg gttcatcgca
    14881 accgcgagcg ttgtatgagc ttccgtagaa atcgatccta acgacatggc gccggtggaa
    14941 aagcgccgca cgatttcctt ggctggttcc acttgatcaa ttggaatgga cttggtcgga
    15001 tcaacgcgaa actcgaacaa tccacgcagc gtcatgtgcc gcttggattg gtcgttaata
    15061 attcttgcgt agtccttgaa cgtctcaata ctgtttgtcc gcgtggagtg ctgcagcttt
    15121 gcaatggcat ccggggtcca catatgttct tcgccgcgga cccggaacgc atactcgcct
    15181 ccggcatcca gtgcatcgac caaaatcgga tcgtcgccaa aggctgccag atgcagacgt
    15241 atcgcctctt cagccacttc aaatatgccg atgccttcta cattcgaggt cgttccagtg
    15301 aaatatttgt ccaccattcc cttcgcaagt ccgacggcct caaaaatttg tgcgccggta
    15361 tacgacatat aggtcgaaat acccattttc gacatgactt tcatcaagcc cttgccgata
    15421 gctttgacat aatttttgat gcctttttcg ccgcgatatt cgttcgggac tggtgcttcg
    15481 gcatgcagaa tagtctccat cgcaacgcag ggatgaacgg cttccgcgcc ataggcagca
    15541 agcaaggcaa aatggtgtac ctcgcgcgcc gatcctgtct cgaccaccaa tccggcactg
    15601 gtacgcaacc ccttccctac caggtgctgg tgaatgccgg acaaggcgag cagcgcggga
    15661 atagccacat gacccgcatc tattctgcga tccgagacga taagaatgct gtaaccagaa
    15721 cgtacggcgt cttctgcttg cgcgcaaagg ctggcaagcc tggcttcaat tgcctgtttc
    15781 ccccaagcca ccggataggt aatgtcaatc tcgtgcgact tgaacttgcc tccagtgtag
    15841 taatcgatat ggcggatcgc atccatctgg ttgaagtcaa gcaccggttg gcccacttca
    15901 aggcgcattg gtgggttggt ctcatcaatg ccaagaaggt tgggcttggg tccgatgaaa
    15961 gacaccagcg acatcaccag gtcttcccga atcggatcga tgggcgggtt ggtgacctgc
    16021 gcgaacagtt gcttgaagta gttgtaaagt gacttctcct tattcgatag cacagccagc
    16081 ggcgagtcat tccccatgga accgatcgct tcttcaccct gctcggccat cggagccaaa
    16141 atgaacttct ggtcttcctg ggtgtagcca aaggcctgct gccgatccag caaagcgacc
    16201 ttgcttggtt cggcgatctt ggtggaaacc ggaagatcat cgagctttat acggatacgc
    16261 tcgatccacg cccgataggg cttggtattg gctaaagtat ccttgagttc cttgtcatca
    16321 atgattcggc cggcctcaag atcgatcagg aacatctttc cgggctgcag tcgccatttc
    16381 tttacgatct tttcttccgg gattggaagc actccggttt ccgaagccat aacaacaaca
    16441 tcgtcgctgg tcacgaggta acgagcggga cgcaaaccat ttcggtccag cgtcgcgccg
    16501 atctgccggc catcggaaaa cgccactgca gcggggccat cccacggctc catcattgcg
    16561 gcgtggtatt cgtagaaggc acgtcgatgg ggatccatca gcgaatgctt ctcccaggct
    16621 tcgggtatca gcatcatcat ggcgtgggcc aacgagtacc cgctcatgat cagaagttcc
    16681 aacgcattat cgaatgatgc ggaatcggac tggccgtcgt agatcaatgg ccaaacctta
    16741 tccaggtctt ttcccagaac gggactgaat atcgcgcctt ggcgagcacg aagccaattg
    16801 acatttccgc gtagcgtatt gatctctccg ttgtgagcga tcattcgaaa cggatgcgcg
    16861 agatcccagg tggggaatgt attggtcgaa aaacgctgat gcaccagggc taaggctgaa
    16921 atcactttag gatcgcaaag atccttgtaa taagtgccga cttgcccggc cagcaacatg
    16981 cccttgtaaa cgagcgtacg tgcggacatg gaaggcacat aaaactcttt gctgtgctcc
    17041 agcttgagcg cctgaatggc atgcccggac accttgcgga tgatatagag cttgcgctca
    17101 agcgcgtcgg tcactgtgac tccagcccct cgtccgataa acacctgccg gatcaccggt
    17161 tcaattttct tgaccgaatc gcccaagccg gcattatccc gtggcacgtc tcgccagccg
    17221 agcaacactt ggccttcgtc ttttatggcc cgttctatct cgtattcgca agccagccgc
    17281 gaagcaggtt cctgcggcaa aaaaaccata ccgacgccgt attctccgga cggtggcagt
    17341 ttgatgcctc ggatagccat ttcgtcccgg aagaactggt ccggaatctg aatcaagatg
    17401 cccgcgccat ctccggccag tggatccgca ccgactgcac cgcggtgggt gagattcctg
    17461 aggatttgca gaccttgctc gataatcgcg tggctacgga cacccttgat gttcgcgata
    17521 aaaccaacgc cgcaagcgtc gtgttcattc gctggatcat agagaccctg ctgcatgggt
    17581 aaagccgaat gagccatcgt cttgcctctt aataagccga gcaaccatct gccgggtcgg
    17641 cagcagattc ttgggagcca taaatggtac cgcgattgct agcgcacttt caatcgccgc
    17701 cgggcggggt cggtaggtgg caaccgagga gcagtatcag gaaaaagggg gtggaccaat
    17761 cactcccgca gcaggatgaa gtccttacgt ctcttctacg gcgaataacc ggcgatgct
//
LOCUS       DATMZQ010000043        12293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3469446, whole genome shotgun sequence.
ACCESSION   DATMZQ010000043 DATMZQ010000000
VERSION     DATMZQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3469446"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..396)
                     /locus_tag="VN496_01485"
     CDS             complement(121..396)
                     /locus_tag="VN496_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXF15642.1"
                     /translation="MNQVESIVAQERELAELIVASLNLEIQPHEIEPAAPLYREGLGL
                     DSIDILEIALAVSKKYGFQLRSDDNDNLKTFSSLRNLNEHVQQHRTR"
     gene            619..1812
                     /locus_tag="VN496_01490"
     CDS             619..1812
                     /locus_tag="VN496_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXF15643.1"
                     /translation="MQPLFLNRYAIASSSAIGLEATVQALQNMRSGLAPCAFETVHLD
                     TFAGQIADTSLAPVRTDLAQFECRNNRVAQLCIRQDGFEEAVIDARRQYGPDRVGVFM
                     GTSTSGILETEIAYRSRDADGMLPRNFPYATTHNTFSVAWFVQSYFALSGPAMAVSTA
                     CSSSAKAFGNAARMIEAGLCDAAVVGGVDSLCLTTLYGFNALGLLSPLACKPFDRERN
                     GISIGEAAGMVLLEKTSSGLKRDGIALLGVGESSDAHHMSTPHPEGLGARMAMERALN
                     SAGIGRDAVDYINLHGTGTRSNDAIEGKAVHAVFGADIPCSSTKGWTGHTLGACGAIE
                     AIICALAVQRGFMPGSANTSMPDQDIPVHYLLATRSAAVDIAMSNAFGFGGTNCSLVL
                     GRART"
     gene            1809..2612
                     /locus_tag="VN496_01495"
     CDS             1809..2612
                     /locus_tag="VN496_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HXF15644.1"
                     /translation="MKLFVESAAVLAPGLAGWVASRPVLAGEQKYEPAPFVLPVAELL
                     PAVERRRTGSTVKLALAAGHEALIAAGRSADSVATVFVSSGNDGDVINDICITLAGSD
                     RQVSPTRFHNSVHNAPSGYWGIAAHSHHPSTSLCAFDWSFAIGLLEAATQLQVERPEV
                     LLIAYDVPYPMPFAGIRRVDQPFGTALLMTRDSTQRSIAGLDIELDRAAKDLSRMADA
                     RLETLRVGNPCASALPLLAAIARGQPETITLQRNAGQTMSVSIRRPNGR"
     gene            complement(2632..3525)
                     /gene="htpX"
                     /locus_tag="VN496_01500"
     CDS             complement(2632..3525)
                     /gene="htpX"
                     /locus_tag="VN496_01500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HXF15645.1"
                     /translation="MVKRIVLFLITNLAVLFVLNITMRLLGIDRMLAQSGGNLGGLLV
                     FAGVIGFGGALISLAMSKWSAKMMTGAQVIEQPQTAAEQWLVETVRRHAQKAGIGMPE
                     VAIYDAPDINAFATGMNRNSALVAVSSGLLTNMTRDEADAVLGHEISHVANGDMVTLA
                     LIQGVVNTFVIFLSRIIGNLVDKVVFKTERGNGPAFWITSIIAELVLGILASIIVMWF
                     SRLREFRADAGGAMLAGREKMIAALQRLKLAQEPPQLPAQIRAFGIAGGGQGLARLFM
                     SHPPLEERIEALRTARAEAPQ"
     gene            complement(3580..5478)
                     /locus_tag="VN496_01505"
     CDS             complement(3580..5478)
                     /locus_tag="VN496_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF15646.1"
                     /translation="MIVLRALQLRRGVKVLFDDASLTFNRGQKIGVTGANGSGKSSLF
                     ALLLGELHQDAGDLEIQSGLVVAHVAQETPSTSQLAIEYVLDGDAELRHLERELAVAE
                     NSHNGTRIAELHEDLHRVGGYAARARAAQLMHGLGFDDAEIARSVEQFSGGWRVRLNL
                     ARALMCRSDLLLLDEPTNHLDLDAIVWLEQWLSGYAGTLLMVSHDREFLDSVANAICH
                     IETQRIRLYAGNYSAFETQRAAQLSQQQATYLKQQREIAHLQSFVDRFKAKATKARQA
                     QSRIKALARMEKIAAAHVDTPFEFAFRKPAVQSDPMLRLDGVSAGYGDVAVLRDIQMT
                     LRPGARLGLLGRNGAGKSTMMKLLAGVLPPLGGQRIEGKGLQIGYFAQHQLEQLRPDE
                     SALWHLTRLSPKTREQELRDFIGGFNFHGDQATEPVGPMSGGEKSRLALALIVWQRPN
                     LLLLDEPTNHLDLEMRHALTLGLQDYEGALVLVSHDRSLLRSTADELWLVDNGKVLEF
                     DGDLEDYARRLRAKEQVLAQDVGEPLVSRKEQKRLEAQERSRRFAQRKPIEARIHSAE
                     QEIQTLSAESQRLEKLIAAPDMYGDARKEDLKRCLLEQAQVAKKLQAAEERWLLLSAE
                     LEALATSG"
     gene            complement(5516..5591)
                     /locus_tag="VN496_01510"
     tRNA            complement(5516..5591)
                     /locus_tag="VN496_01510"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5556..5558),aa:His,seq:gtg)
     gene            complement(5627..5703)
                     /locus_tag="VN496_01515"
     tRNA            complement(5627..5703)
                     /locus_tag="VN496_01515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5667..5669),aa:Arg,seq:tct)
     gene            complement(5733..5809)
                     /locus_tag="VN496_01520"
     tRNA            complement(5733..5809)
                     /locus_tag="VN496_01520"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5773..5775),aa:Pro,seq:tgg)
     gene            5946..6368
                     /gene="dksA"
                     /locus_tag="VN496_01525"
     CDS             5946..6368
                     /gene="dksA"
                     /locus_tag="VN496_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HXF15647.1"
                     /translation="MASEFHTNFKPYVPKKGEQYMSPKQLEHFRKILNQLKVELSQDI
                     DRTVHTMQDEATVFADPNDRASQESDMALELRNRDRERKLIKKIDETIGKIDGGEYGY
                     CESCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            6602..6904
                     /locus_tag="VN496_01530"
     CDS             6602..6904
                     /locus_tag="VN496_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXF15648.1"
                     /translation="MFDKIQNPREIRLRLNLNQQEFWGQIGVTQSGGSRYESGRNMPK
                     PVRELLRLVHVDQVDLKKINKGDVALISYLKLSQPALYRRLKSEASTRGTRRRARR"
     gene            complement(6908..7771)
                     /locus_tag="VN496_01535"
     CDS             complement(6908..7771)
                     /locus_tag="VN496_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXF15649.1"
                     /translation="MSEAVRSRLTVTDHSRTSAGLLYIYPVVSRRAGGVSVGINLNTN
                     NACNWRCIYCQVPDLVKGAPAPIDMDQLRRELLGFLEEVIHGDFLTTRVPAGARRLND
                     IALSGNGEPTSAKEFGEVINLIGEARDLAKVPERVKTVLITNGSLMHRVNVQRGVKQL
                     ARLNGEIWFKIDRATDSGIRLINNARVSMSRVREHLAAAARLCPTWIQTCLFALDGQG
                     PDDRERQAYLDFVRECLRDGIALRGVLLYGLARQSFQPEASRLTPLPPEALESFAQHI
                     RALGLDVKVTP"
     gene            complement(8134..8805)
                     /locus_tag="VN496_01540"
     CDS             complement(8134..8805)
                     /locus_tag="VN496_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15650.1"
                     /translation="MSEHAVEAARYALLRRLAPAIRHRVVGKLHPIGLIAEALEWQIQ
                     EAAPNLKNLRESIARVSSMSRAAMQSFTTEMTWLTHEDSASVTIAAGIEDCLALLHTD
                     FELRGFSIRNEAKAPAVRVSMNALRNVLAAVMIAISDSAPAPAILVLAAAISGTHASI
                     SILIHPAEAVSMFETSDAYRRLEWDDVQVLAAAESVELSRRGAQVEMRYPIQADVNQS
                     SRAIG"
     gene            9097..9822
                     /locus_tag="VN496_01545"
     CDS             9097..9822
                     /locus_tag="VN496_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF15651.1"
                     /translation="MGDKEATYSQFRTSGHPFDASHEATREQPSRIKILIVDDHAIVR
                     AGLKQFIANEPDMVVEGEADSGGQALSMIRDGHWDIVILDVSMPDQNGVDTLRRIKSL
                     EPQLPVLILSGFSENQYAVNLLRAGASGYLSKDGDSRDLITAIRMVVQGGKYVTPALT
                     EILVNDLAARQEERPPHSLLSEREFQVFCHLAAAIGVSKIAQKLGLSVKTVSTYRTRA
                     LEKMKLTSNAELTQYAVRHRLID"
     gene            9850..10332
                     /locus_tag="VN496_01550"
     CDS             9850..10332
                     /locus_tag="VN496_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15652.1"
                     /translation="MRLTRVRERKPVQDAAPIVMTRIIDAAMDARKRQPAAHLVEAEA
                     PEPIAPLKVFLIDESPFVRERVIEKITSTGRIALAGFADNERTAIEKLQDVHCDAIIF
                     QLPGRHGTWSQILRLLRVRGSYRPYVIVFGDYGRRNKPQDLDYLRDILEGLSPGGHRQ
                     "
     gene            complement(10387..11265)
                     /locus_tag="VN496_01555"
     CDS             complement(10387..11265)
                     /locus_tag="VN496_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXF15653.1"
                     /translation="MRDGVDIRANNLLATLVGSGCEELKAHLELVALPVGKVLYESGG
                     AMRHVYFPTTSIVSMLYIMNDGTSDEIATVGSEGFIGIQLFLGCETIPSRALVRNAGS
                     AFRLEAGLLQKRFTQSSAVQHLLLRYMQSLLAQISQTAACNRHHSIHQRLCRWILSSL
                     DRLPSSELQTTHESIGEMLNVRRESITQELGELKVAGLIHCQRGRITVINRLKLEARA
                     CECYGGIKREFNRLLAGLNSSPVRNGLEELWQARSTDAASQISFGEVPDYTSGPEAYA
                     RQDEEYLGRHQPAVSA"
     gene            complement(11603..12238)
                     /locus_tag="VN496_01560"
     CDS             complement(11603..12238)
                     /locus_tag="VN496_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF15654.1"
                     /translation="MIKILIADDHAVVRAGVRQFIAGHDDLNVVAEASNGAEVIDYIR
                     KSEIDVVVLDISMPGHSGIDTLKSLKHVRPELPVLILSGFAEEQYAVNLLRAGAAGYV
                     NKEAAPAQLVAAIRTVAQGRKYVSPALAQMLADRLSIKDDEQPVHAELSQREFQIFCK
                     LVKGEGVTQIADELFLSVKTVSTYRSRILEKMGMKSNADLTYYAIKNKLIT"
BASE COUNT         2625 a         3572 c         3483 g         2613 t
ORIGIN      
        1 agcgactctc caaggtgact gccgggatga tagaccgtga tgaaataggc cagtgcctgg
       61 taagacatca ctgcagcagc gaacagcatc actttagccc agcgggtccg cggcgaacga
      121 ctaacgggtt ctatgctgct ggacatgctc gttcaggttg cgcagcgagc tgaaggtctt
      181 caggttgtca ttgtcatcgg aacgcaattg aaagccgtat ttcttggata ccgccagcgc
      241 gatttcaagg atatcgatgg aatcaaggcc gagtccttca cgataaagcg gcgccgcggg
      301 ttcgatttca tgtggctgga tttccagatt cagggacgcc acgatcaact ccgcaagctc
      361 ccgctcctgg gctacgatgg attcaacttg attcatcaga ttcattattc aaacttggtt
      421 cattgccagg cgtttgatcc ggcttgccgg ccggcgcttc cggtccgcag aattataacc
      481 gctccgccct tacggaccac ttcagtgcaa cgcgcaaatg cgattgacgc cggtgtctcg
      541 ggcgacgtaa cctcggttgc cggaaatccg cgagtggcac cgggcagcgt tactgcccgc
      601 agaacgggaa atcattgagt gcaaccgctt ttcctaaatc gttacgccat tgccagtagc
      661 agcgcaattg gccttgaagc gaccgtgcag gccttgcaga acatgcgttc agggcttgcg
      721 ccctgcgcgt tcgaaaccgt ccatctcgac acctttgccg ggcagatcgc cgacaccagc
      781 ctggcgcctg tacgcaccga tctggcgcaa ttcgagtgcc gcaataaccg ggtcgcgcaa
      841 ctctgcatcc gtcaggatgg attcgaggaa gcggtcatcg atgcccggcg acaatacggg
      901 ccggaccggg tcggcgtttt tatgggcacg agcacttcgg ggattcttga aaccgaaatt
      961 gcgtatagaa gccgcgatgc ggatggcatg ttgccgcgca acttccctta tgcgacgact
     1021 cacaacacat tctcagtcgc ctggttcgtt cagtcttact tcgctctctc ggggccagcg
     1081 atggcggtat ccactgcctg ttcttcgagc gcaaaggcgt tcggcaatgc tgcgcgcatg
     1141 atcgaggccg ggctgtgcga cgctgcagtc gtaggcggcg tcgacagtct gtgcctcact
     1201 acgctgtatg gattcaatgc gctgggcctg ctgtctccgc tcgcatgcaa gccgttcgat
     1261 cgcgaacgca atggcatttc catcggcgag gcagccggta tggtgttgct ggagaaaaca
     1321 tcctctggct tgaagcggga tgggatcgct ctgctgggcg tcggagaaag cagcgacgcg
     1381 caccacatgt cgacgccaca tccggaaggt ctcggggcgc gcatggccat ggaacgcgca
     1441 ctgaacagcg ccggaattgg acgcgatgcg gtggactaca ttaatttgca tggcaccggc
     1501 acacgcagca acgatgccat cgaaggcaag gcggtgcatg ccgtgttcgg agctgatatt
     1561 ccctgcagtt ctaccaaagg ctggaccggc catacgttgg gcgcttgcgg cgccatcgaa
     1621 gccatcattt gtgcactggc cgttcaacgc ggtttcatgc caggcagcgc caacacgagc
     1681 atgccggacc aagacatccc tgttcattac ctgctggcca ctcgaagcgc ggcggtcgac
     1741 attgcaatga gcaatgcatt tggattcggt ggcactaact gcagccttgt actcgggcgc
     1801 gcgcgcacat gaaactgttt gtggaaagcg ccgccgttct ggcgccaggg ctcgcaggct
     1861 gggttgccag ccgtccggta cttgctggtg aacaaaaata tgaaccggca ccgttcgtgc
     1921 tcccggttgc ggaactcttg cccgctgtcg agcgtcgtcg caccggctct acagtcaagc
     1981 tggcgctcgc agccggacac gaagcactca tcgctgccgg gcggtccgcg gattccgtcg
     2041 cgacggtgtt cgtttcctca ggaaacgatg gtgacgtgat taacgatatc tgcatcaccc
     2101 tggccggatc cgatcggcag gtttctccaa cgcgcttcca caattcggtg cacaacgcgc
     2161 cgtccgggta ttggggcatt gcggcacatt cacatcatcc ctcaacgagc ctgtgcgcat
     2221 ttgactggag ctttgccatt ggactcctgg aagcagccac gcagctgcag gtggagcggc
     2281 cggaagtgct gctgattgcc tatgacgtac cttatcccat gccgttcgcc ggcatacgcc
     2341 gtgtcgatca gcccttcggc actgcgcttt tgatgacacg ggatagcacc caacgcagca
     2401 tcgccggact cgacatcgaa ttggatcgag cagcgaagga cttgtcccgc atggcggatg
     2461 ccaggcttga aactttgcgc gtgggcaatc cgtgcgcaag tgcgcttcca ctgctcgcag
     2521 ccatcgcgcg tgggcaaccg gagacgatca ctttgcagcg caatgcggga caaacgatgt
     2581 cggtttcgat acgccgaccg aacggtcgtt gagccctgaa tccggactcg gttactgtgg
     2641 cgcttcagcg cgcgcggtgc gcagcgcctc gatgcgttct tccagtggcg gatgcgacat
     2701 gaacaagcgt gcaaggccct gtccgccgcc ggcgatgccg aatgcacgga tctgcgccgg
     2761 caactgcggc ggttcctgcg ccagtttgag tcgctgtaat gccgcgatca tcttctcgcg
     2821 tcccgcgagc atcgcaccac ctgcgtcggc acggaattcg cgcagccggc taaaccacat
     2881 cacgataatg ctggccagga tgccgagcac cagttcggcg atgatcgaag tgatccagaa
     2941 tgccggcccg ttgccacgtt cggtcttgaa gaccaccttg tcgacgaggt tgccaatgat
     3001 gcgcgacagg aaaatgacaa acgtgttcac gacgccttga atcagcgcca gcgtgaccat
     3061 gtcgccgttg gcgacgtggc tgatttcgtg gcccagcacg gcgtcggctt cgtcgcgcgt
     3121 catgttggtg agcaatccgc tgcttacagc gaccagcgcg ctgttgcggt tcatgccggt
     3181 ggcgaacgcg ttgatgtcgg gggcatcgta tatcgcaacc tcaggcatcc ctattccggc
     3241 cttctgcgcg tgccggcgga ccgtctcgac cagccattgt tctgctgccg tttgcggctg
     3301 ttcgattact tgcgcgccgg tcatcatttt ggcagaccac ttcgacatgg cgagcgaaat
     3361 cagtgctcct ccaaaaccga tgactccggc aaacaccaac agcccgccca gattgccgcc
     3421 cgactgtgcg agcatgcgat cgatgcccag caggcgcatg gtgatgttga gaacgaacag
     3481 caccgccagg ttggtaatca gaaacagcac aatgcgtttg accacgtatt gccctttcgc
     3541 aggtggatta aatgcagttc atgtgttatg tacagttctt taaccgctgg ttgccaaggc
     3601 ttccagttct gcggaaagca acaaccaacg ctcttcagca gcctgcaatt tctttgcaac
     3661 ctgcgcctgc tccagcaggc agcgcttcag atcctctttg cgcgcatcgc cgtacatatc
     3721 gggggcggcg atcaactttt caagacgctg actttccgcg ctcagcgtct ggatttcctg
     3781 ttccgccgaa tgaatgcgcg cttcaatggg ttttcgctgc gcaaagcggc ggctacgctc
     3841 ctgcgcctcc aggcgctttt gctccttgcg actgaccaga ggttcgccaa catcctgcgc
     3901 cagaacctgc tctttggctc tcaagcgcct ggcatagtct tccagatcgc cgtcgaactc
     3961 cagaacttta ccgttatcca ccagccataa ctcatcggcc gtcgaacgca acaggctgcg
     4021 gtcgtgcgag acgagtacca gcgcgccttc gtagtcctgc agtcccaggg tcagggcgtg
     4081 ccgcatctcc aggtcaagat gattggtcgg ctcgtccaac agcagaagat ttggccgttg
     4141 ccatacgatc aacgctaggg cgagacgtga cttctctccg cccgacatcg gccctaccgg
     4201 ctcggttgcc tggtcaccgt ggaaattaaa gccgccaatg aaatcacgca gttcctgttc
     4261 acgtgttttt ggcgagaggc gcgtcagatg ccacaacgcg ctctcatcgg gacgcagctg
     4321 ctcgagctga tgctgtgcga aatagccaat ctgcaagccc ttgccttcga tgcgctggcc
     4381 ccctaacggc ggcagcacgc ccgcgagcaa cttcatcatt gtggacttgc cggcgccgtt
     4441 gcgtccgagc aggccaaggc gcgcgccggg acgcagcgtc atctggatgt cgcgcaaaac
     4501 tgccacgtcg ccatatccgg ccgaaacgcc atccaggcgt aacatcggat cagattgcac
     4561 ggccggcttg cggaaggcga attcgaatgg cgtatccacg tgcgccgcgg cgattttttc
     4621 catgcgtgct agcgctttga tgcggctctg cgcctgccga gctttggtcg ccttggcctt
     4681 gaagcgatcg acgaaggact gaaggtgcgc gatctcacgc tgctgcttca ggtaagtcgc
     4741 ctgctgctgc gacagttgcg cggcacgctg cgtctcgaac gcagaatagt tgcccgcgta
     4801 caggcgaatg cgttgagttt cgatgtggca aatggcgttg gccacgctat cgagaaattc
     4861 acgatcatgt gaaaccatca acaaggttcc ggcatagccc gagagccact gctccagcca
     4921 cacgatggcg tcgaggtcca ggtgattggt cggttcatcc agcagcaaca ggtcggaacg
     4981 gcacatgagg gcgcgcgcca gattcaggcg cacccgccaa ccacccgaaa attgttcgac
     5041 cgacctcgcg atctcggcgt catcgaaccc caggccatgc atgagttgtg ctgccctggc
     5101 tcgcgccgca tagcctccga cccggtgcaa atcctcgtgc agttcggcaa tgcgcgtgcc
     5161 gttatgcgaa ttttctgcaa ccgcaagctc gcgttcgaga tgacgcagct ccgcatctcc
     5221 gtcgagaaca tactcgatgg caagctggct ggtagacggt gtttcctgcg ctacgtgagc
     5281 gacaacaagg ccggactgga tctccagatc accggcgtcc tgatgcagct caccgagcaa
     5341 cagcgcgaac aggcttgatt tgccgcttcc attggcgccg gtcacgccga tcttctgccc
     5401 gcggttgaac gtcaggctcg cgtcatcgaa caaaactttc acgccgcggc gcaattgcag
     5461 ggcgcgcagc actatcatga tttgcagttc atattgtcag gagcaacacg aaggatgggg
     5521 tggctgatgg gactcgaacc cacgacaacc ggaatcacaa tccgggactc taccagctga
     5581 gctacagcca ccacttgaaa ccttatgacc agaaagcggg gggcactggc gtgcccgaca
     5641 ggaatcgaac ctgtaacccc cagcttagaa ggctggtgct ctatccggtt gagctacggg
     5701 cacgtttctg accgggcaga ccatcgcttt tctggtcggg gtggagagat tcgaactccc
     5761 gacatcctgc tcccaaagca ggcacgctac caggctgcgt tacaccccgg agggccagaa
     5821 ctatacctat cagggggtta aaggtcaatt tagccgcgga gctttgcagc agcataaaca
     5881 ttagttgcat gcctgaatta tttctgatat taaagcgcac ttttccggac ccgaacctgt
     5941 aaaaaatggc atccgaattc catacgaact tcaagccgta cgtgccgaaa aagggcgagc
     6001 aatacatgag cccgaaacaa ctcgagcatt tccggaaaat tctcaaccag ctgaaagtcg
     6061 agctgtcgca ggacattgac cgcaccgtgc acacgatgca ggacgaagca acggtcttcg
     6121 ccgatcccaa tgaccgggca agccaggaat ccgatatggc attggagctg cgcaatcgcg
     6181 atcgcgaacg caagctgatc aagaagatcg acgaaacgat cgggaagatc gacggcggcg
     6241 aatatggtta ttgcgaaagc tgcggcgtgg agatcggcct taaacggctg gaggctcgcc
     6301 cgacagcaac gctttgcatc gattgcaaaa cgctggacga actgagggaa cggcaagtcg
     6361 caaagtaaat tttctcgtcg cttgctgttt ctgcggcaaa aagcacacct aaacccggaa
     6421 cacgtcaacg cccttcagcg aagggcgttg ttacattcgg catgccagct agccagttgt
     6481 catggctcgc taaatggaac tttgtttgcg gcatccggac caatacggga tgcgggcggc
     6541 atcgcacggt cttaggcatt gcgcgtccga tgaacaatca accaccaaga ttgggatcag
     6601 catgttcgat aaaattcaaa atccccgcga aatccggttg cgcctgaatt tgaaccagca
     6661 ggaattttgg ggacagatcg gtgtaacgca aagcggcggg tcccgctatg agagcggccg
     6721 caatatgcca aaaccggtgc gggaacttct ccgtctggtt cacgtggatc aagttgatct
     6781 gaaaaaaatc aataagggcg atgtcgcttt gatttcgtac ctcaagttgt ctcaacccgc
     6841 gctttatcgc cgcctgaagt ccgaggccag tacccgcggc acacgccggc gcgcgcgccg
     6901 ctaggcgtca gggcgttact ttgacgtcca ggcccagcgc acgaatgtgc tgggcaaagc
     6961 tctccagcgc ctcgggcggc agcggcgtga ggcgcgatgc ttccggttgg aaggactgcc
     7021 tcgcaaggcc gtaaagcagc actccccgca acgcaatccc gtcgcgcagg cattcgcgca
     7081 caaagtccag atatgcctga cgctccctgt catctggacc ttgcccatcg agcgcaaaca
     7141 gacatgtctg gatccatgtc ggacaaagcc gcgcggcagc cgccagatgt tcgcgcaccc
     7201 ggctcatact gacacgtgcg ttgttgatta gtcggatgcc ggaatcggtg gcgcgatcga
     7261 ttttgaacca gatttctcca ttgagccggg ccagctgctt cactccgcgc tgaacattga
     7321 cccggtgcat cagactgcca ttggtaatta aaacagtctt tacccgttca ggcaccttcg
     7381 ccagatcacg ggcttcacca atcaggttga tgacttcacc gaactccttg gcgctggtag
     7441 gttctccatt gcccgacagg gcaatgtcat ttaaccgccg cgcccctgcg ggtacacggg
     7501 tcgtgagaaa atctccgtgt atgacctcct ccaggaagcc aagcagttca cggcgcaatt
     7561 gatccatgtc gatcggcgct ggcgcgcctt tgaccagatc cgggacctga cagtagatgc
     7621 agcgccaatt gcacgcgttg ttggtattga gattgatgcc gaccgatacg ccgcccgctc
     7681 gccgcgacac cacgggatag atatagagca gccccgcact cgtacggcta tggtcagtga
     7741 ccgtcaagcg gctacgcacg gcttcggaca cgagatcgct ccaatataca gtcgtgaatt
     7801 cacgcttacg cgtccctccg gcgcaggaaa agtcggataa cgacatcctg cacgttgctg
     7861 ggcgtgggaa tcagcgagct ttcagatgta ttcagtttta aagctctcca ccgcatgctt
     7921 ccggaatact gccaagcgga gaaagctctg cttagtatgc catgccgcac gcaggctttc
     7981 gcgctcgtag cccgtgcatt atgagggggt ttccgcggca gctgacaaga aacgccaaac
     8041 tcacacttga tgtgccggag cggtatctgc gacaggcaac gacgtgcggg cgcgctctcg
     8101 aaactctgct caaagactca ctgagacagg aattcagccg atcgcccgcg aggattgatt
     8161 aacatctgcc tgaatggggt aacgcatttc cacctgtgcg ccacgtcgag aaagttcgac
     8221 cgattcggcc gccgccagca cctgcacatc gtcccattcg agccggcggt aagcatcgct
     8281 ggtttcgaac atcgacactg cttccgcagg atgtatcagt atcgaaattg acgcatgtgt
     8341 tccggatata gcggcagcta gaacaagtat cgcgggagct ggggcggagt ctgatatcgc
     8401 aatcattacc gcggctagca cgttacgaag cgcattcatc gaaacgcgca cggcaggcgc
     8461 ctttgcttca ttcctgatag aaaagccacg cagttcgaag tccgtatgca gcaatgccag
     8521 acaatcttca atgccggcag caatagttac actagcgctg tcctcgtgag tcagccaggt
     8581 catttcggtt gtaaacgact gcatcgccgc cctggacatg ctgctgaccc ttgcgatgct
     8641 ttcccgcaaa tttttaaggt ttggcgcggc ctcttgaatc tgccattcca gcgcttccgc
     8701 tatcagccct atagggtgca acttgccaac cacgcgatgc cgtattgctg gcgctaatcg
     8761 tcgcaatagg gcatagcggg cagcttcaac tgcatgttcg gacattcttg ttttattttc
     8821 ggtaaggatt gtatttatac agcgtcggct tccggctctc tcccgtcagg gcccggcgca
     8881 cgccaagccg gcgcacccga gccgtactcc agtgtacgga aaatgatatc agcggcgtgc
     8941 aaccgattat gtaggagttt tccgacaaca gatttccgat aggacggatt acgctccggc
     9001 gcctgctctg gcagcatcca tattgtgacg cggcccttct ccttgcggaa atccaggcaa
     9061 gcgaagccac atttcaaaca agacgatcgg tggaaaatgg gcgataaaga agccacttac
     9121 agccaattta gaacgtccgg gcaccctttt gacgctagcc atgaggcaac tcgagaacag
     9181 ccttccagga tcaaaatact catcgttgat gatcacgcaa ttgtccgggc cgggttgaag
     9241 cagtttattg caaacgagcc cgacatggtg gttgaaggag aggcggatag cggtggccag
     9301 gcgctgtcga tgattcggga tgggcactgg gatatcgtga tactggatgt ttcgatgcct
     9361 gatcagaacg gtgtcgacac gctgaggcga atcaagagtc tggaacccca gttgccggtc
     9421 ttgatcctgt ccggtttttc cgagaatcaa tatgcagtca atctgctgcg tgccggcgcc
     9481 agtggttatc tcagcaagga tggcgactcg cgcgacctca tcactgccat tcgcatggtc
     9541 gtgcagggag gtaagtacgt aacgccggcg ctgacagaga ttctcgtcaa tgacctcgct
     9601 gccaggcagg aagagcgccc accgcacagc ctgctgtccg agcgcgagtt tcaggtcttc
     9661 tgccaccttg ctgctgcgat tggcgtatcc aagatcgcac agaagctggg gctttcggta
     9721 aagacagtca gtacctaccg tacgcgcgca ttggagaaaa tgaaattgac cagtaatgcc
     9781 gagcttactc aatatgccgt gcggcatcgc cttatcgatt aaacgcgcgt aaacgatccg
     9841 gacagtatcg tgcgcctaac cagggtgcgg gaacgtaaac cggtgcaaga tgcggcgccc
     9901 atcgtcatga cccgcataat cgatgcagcc atggacgccc gaaaacgtca gcctgctgcg
     9961 cacctggtcg aagccgaagc tcctgagccg atagcgccgc taaaggtatt cctgattgat
    10021 gaatccccgt ttgtgcgcga acgcgtaatc gaaaagatca cctcgacggg ccgcatagcg
    10081 ctagcgggct ttgcggataa tgaacggacg gccatagaga aactgcagga cgttcactgc
    10141 gacgcaatta tttttcagtt acccggccga cacggaacct ggtcgcagat tttacgattg
    10201 cttcgcgttc gcggttcgta taggccgtac gtgatcgtgt tcggtgatta cggacgacgg
    10261 aacaagccgc aagatttgga ctacttacgc gacattctgg aaggtctttc gccaggcgga
    10321 caccggcaat aagcgatgtg cgacgccttc ggcgctcatc cgatcatttc cggggcgcac
    10381 tttttgttat gcggaaacgg cgggctggtg acggccgaga tactcctcgt cctggcgtgc
    10441 gtaggcttcc ggcccggaag tgtagtcagg aacttcgccg aaactgatct gcgaagcggc
    10501 atcggttgaa cgcgcctgcc agagttcttc caggccattg cgcaccggag aactgttcaa
    10561 cccggcgagc aagcggttga actccctttt gatcccacca taacattcac atgcacgggc
    10621 ttccagcttg agccggttga ttacggtaat gcggccgcgc tgacagtgga tcagtcccgc
    10681 taccttcagc tcgcccagct cctgggtgat gctttcgcgc ctgacattaa gcatctcccc
    10741 gatcgattca tgggtcgtct gaagctcgct ggaaggcaac cggtcgaggc tggacaggat
    10801 ccaccggcac agacgttgat gaatggaatg gtggcggttg caggcagcag tctgggatat
    10861 ctgggccagc agtgactgca tatagcgcag caggagatgc tgaacagcgg aagactgggt
    10921 gaagcgtttt tgcaacagac cggcttccag gcggaaggca ctgcccgcgt tgcggaccag
    10981 agcacggctc gggatggttt cgcaaccgag aaacaattga atgccaatga acccttcact
    11041 gccgacggtc gcgatttcat ccgatgtccc gtcgttcatg atgtaaagca tcgaaacaat
    11101 ggatgtggtc gggaagtaga catggcgcat cgccccgccc gattcgtaaa gtacctttcc
    11161 caccgggagc gccacgagtt ccaggtgtgc cttcagttct tcgcagcctg agccaaccag
    11221 cgtggcgagc aggttgttcg cgcggatatc gacgccatca cgcatgataa accccggctt
    11281 tcggcaacag gtccaccggg tgaagtccgg gccgggtggt attggagact gattccgtcg
    11341 cagtctctgc cggcagatgc atgctatgcc gtgattccgt attggtattg ttttccatac
    11401 actggcctct caggatgtac gtatgggatc atcccgaaag gccctgtccc gcaatatcag
    11461 tcaacaaccc cttgaggtgt aggacaaagc atgacagctc gtccctgaag ccatgcaggg
    11521 ctccgcaaga gcgttttatt ggtttgtcgc ggcagggcgg actccggacc ggtgtccgcg
    11581 gaaggcagga ttccagattt cactaagtaa tgagtttatt ttttatggcg taatacgtta
    11641 aatcggcatt gcttttcatt cccatttttt ccaggatacg agagcgatag gtgctaacag
    11701 ttttcacaga cagaaacagt tcgtccgcga tctgcgtaac gccctcccct ttgaccagtt
    11761 tgcagaatat ctggaattcc cgttgcgaca gttcggcgtg caccggttgc tcgtcgtcct
    11821 tgatagacaa tcggtccgca agcatctgag ccaaggccgg gctgacgtac ttccggcctt
    11881 gcgccaccgt gcggattgcc gccacgagct gcgccggcgc ggcttctttg ttgacgtagc
    11941 ccgctgcacc ggcacggagc aaattaaccg cgtattgttc ctcggcaaag ccggaaagga
    12001 tcaataccgg caattccgga cggacatgtt tgaggctctt gagcgtgtcg ataccgctgt
    12061 gtccgggcat cgaaatgtcg agcacgacca catcgatttc actctttctg atgtagtcga
    12121 tgacttccgc gccgtttgat gcctccgcaa ctacattgag gtcgtcgtgg cccgcaatga
    12181 attgccggac tccggcgcgt accacagcat ggtcatcagc gataaggatt ttaatcatgg
    12241 cgatcgcaga gaattcctag cgtgtttcga attcaggtcg ttattcgccc tgt
//
LOCUS       DATMZQ010000044         3860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4159660, whole genome shotgun sequence.
ACCESSION   DATMZQ010000044 DATMZQ010000000
VERSION     DATMZQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4159660"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VN496_01565"
     CDS             <1..641
                     /locus_tag="VN496_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF15655.1"
                     /translation="FQLVRARLIALEGNSRDAVTNLDRALQERKFSSEVAVRYGLVVA
                     LLRAGDKARARKEMATLSALKPKSPMIDALAAQTLIAGGEVEKGLAAYRDSLRINPSR
                     TALIYGYTNALLAVNKPADAIQFLSERADGRPADPQLYEFQARAYTALGRRLLQHRAL
                     AEVYALRGNLPAAIEQLQIAQKAGDGDFYQKSSVEARLKELMAADSENRHKP"
     gene            788..2083
                     /locus_tag="VN496_01570"
     CDS             788..2083
                     /locus_tag="VN496_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXF15656.1"
                     /translation="MEKIGKYEVIKELGRGATSVVYLANDAFALRQVAIKLVRADALR
                     DREHGRRFHKLFLAEASLAGKLSHPHLVAIYDAVSDEEGSYLVMEYVAGGTLEPHTQP
                     DKLLTITSIVEIIFKCCKALDYAHRHGIIHRDIKPANILLTAETDIKISDFGAALSVT
                     SETTQVSGIGSPAYMSPEQLQETTLNHQTDIFSLGVVMYQLLTGSLPFKSSNNYSMVY
                     QIINVDPSPASLLRPDTPAVVDFIVKRALEKDLAKRFQTWEEFSQALADTFNNLQKPE
                     EVVPETEKFSILRALEFFTTFQDVDLWQVLRISSWIRHPSGSEIIKEGDVGTSFYVVA
                     TGEVKITKQGKLLNILKTGECFGEMAYLGKRAFQRTASVTSVNDITLIEISAESLEQA
                     TEGCKHAFNAAFLQLLVGRLEGANARLSQLLLDRNISLF"
     gene            2149..2394
                     /locus_tag="VN496_01575"
     CDS             2149..2394
                     /locus_tag="VN496_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXF15657.1"
                     /translation="MNAEPGISCDRELDARGLNCPLPILRTKKSLNDMASGQVLKITA
                     TDPGSVRDFEAFCKQTGNLLLLSDHRNGEYVFMLRKK"
     gene            2394..3026
                     /gene="coq7"
                     /locus_tag="VN496_01580"
     CDS             2394..3026
                     /gene="coq7"
                     /locus_tag="VN496_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HXF15658.1"
                     /translation="MPPPNIDKLIVNFDRALRTLFAGASSVRRFPGGELNESNLDDKD
                     RRNAAALMRVNHAGEICAQALYHGQALTARNAEVQQALESAAIEETEHLAWTMQRITE
                     LNGKVSVLNPVLYASSFAIGTVCGLLGDKWNLGFLAETEKQVEGHLEGHLDRLPHEDN
                     RSRAILLQMKEDEAQHARTALRHGAAELPLPARKMMRAASKLMTRVTYWV"
     gene            complement(3029..3445)
                     /locus_tag="VN496_01585"
     CDS             complement(3029..3445)
                     /locus_tag="VN496_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXF15659.1"
                     /translation="MKVRVKWIEHVAFVGETESSHAIVMDGAAEGGGRNLGPRPMETM
                     LAGAGGCSAYDVVTILKKARQDVSDCVLEIDADRAPIDPKVFTRIHFQFTITGRNIKP
                     ENVQRAIELSAHKYCSASIMLGKTAAITHDFTIVEA"
     gene            3721..>3860
                     /locus_tag="VN496_01590"
     CDS             3721..>3860
                     /locus_tag="VN496_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HXF15660.1"
                     /translation="MADSDVDRGKRRFLIATTSVVGGIGGAAAIVPFAMSMAPSARAR
                     AAG"
BASE COUNT          919 a          997 c         1076 g          868 t
ORIGIN      
        1 aattccagct cgttcgcgcg cggctgatag cgttggaagg gaatagccgc gatgcggtga
       61 caaatctgga ccgggcgttg caggaacgaa aattcagcag cgaggtagcg gtacgctatg
      121 gtttggtcgt cgctttactg cgcgcaggtg ataaagctcg ggcacgtaaa gaaatggcaa
      181 cactgagcgc cttgaagcct aagagtccga tgattgatgc gttggccgcg caaacactga
      241 ttgcaggggg ggaagtggaa aagggactgg cggcctatcg cgacagtctg aggattaacc
      301 cgagccgcac agcgttgatt tacggctaca caaacgcatt gcttgccgtt aacaagccgg
      361 cggatgccat tcagtttttg agcgagcgag cggatggccg ccctgccgac cctcagcttt
      421 atgagttcca ggcccgcgcg tatacggcgt tgggtaggcg gctgctgcag catcgcgcgt
      481 tggcagaggt ttacgcgcta cgaggtaatt tgcctgcggc aatagaacag ctccagattg
      541 cacaaaaggc gggagacggg gacttttacc aaaaatccag tgtcgaagcg cgcctgaagg
      601 agctgatggc ggccgacagc gaaaaccggc acaaaccctg actgccacaa ggcgttagcg
      661 tactgagtgc gttccggcca gctgggtttg ttaatgcgcg ccgatgatag ctaaatcctc
      721 gccttactgc cgcgttttcc tatcataatg aagtctccgg atggcgcaca gtcccgttgt
      781 tgttcgcatg gaaaaaatcg gcaaatacga agtcatcaag gagctcggca ggggagcaac
      841 cagcgtggtt taccttgcca atgatgcctt tgcgctgcgg caagtggcca tcaagctggt
      901 tcgcgctgat gcacttcgtg acagggagca tggccggcgt ttccacaaac tgttcctggc
      961 ggaagcttct ctcgctggca agctatccca tccacatctg gtcgccatct atgatgcggt
     1021 atcagacgag gaaggcagct acctcgtcat ggaatatgtt gcaggtggga ctctggaacc
     1081 acatacacag cccgacaagc ttctcaccat tacgtcaatt gttgaaatca tcttcaaatg
     1141 ctgcaaggcg ctcgactacg cgcatcggca cggcatcatt cacagggaca taaaacctgc
     1201 caatattcta ctgacggcgg aaacggacat taaaatttcc gattttggtg ccgcgttatc
     1261 ggtgacttcc gaaacaacac aagtctccgg aatcggttct ccggcgtaca tgtcacccga
     1321 acagctgcag gaaacgaccc tcaatcatca gaccgatatt ttttcgctcg gcgtggtgat
     1381 gtatcaattg ttaacaggtt cgctgccgtt caagtcgagc aataattaca gcatggtcta
     1441 ccagattatt aatgtcgatc cctcgccggc aagccttttg cggcctgaca ctccggcggt
     1501 agtcgacttt attgtgaaac gcgctttgga aaaagatctg gcgaaacgtt tccagacatg
     1561 ggaagaattt tcccaggcat tggcggatac cttcaacaat ctacagaagc ccgaagaagt
     1621 cgtaccggag acggaaaaat tcagtatctt gcgcgcgctc gagttcttta cgacttttca
     1681 ggacgttgat ctttggcagg tgttgcgcat atccagttgg ataaggcatc catccggaag
     1741 tgaaattatc aaggaaggcg atgtcggtac aagtttttat gttgttgcta ccggagaagt
     1801 gaaaattaca aagcagggaa agctgctcaa tattttaaaa accggagaat gttttggaga
     1861 gatggcttat ctcggcaagc gcgccttcca gcgtacggcg agtgtcacgt cggtgaacga
     1921 cattaccttg attgaaatca gcgcggaatc cctggagcag gccaccgaag gatgcaaaca
     1981 tgcgttcaat gcggcctttc tgcaactgtt ggttggacga ctggaaggcg ccaatgcccg
     2041 tttgtcccag ttgctgctcg accgcaacat cagtctcttc tagtgccaat caagcgcctc
     2101 tgtccgcacg caagatggcg atattcttca gttcaatgat gaaacgtaat gaacgctgaa
     2161 ccaggaatca gttgtgatcg ggaactcgat gctcgggggc ttaattgtcc attgccgatc
     2221 ttgcggacca aaaaatcact caatgacatg gcgtccggcc aggtgctcaa aatcaccgcg
     2281 accgatcccg gatccgtacg cgattttgag gcattctgta aacagacagg caatctcctg
     2341 ttgctgtctg accatcgcaa tggcgagtac gttttcatgt tgcgcaagaa ataatgccgc
     2401 cgcccaacat tgacaaattg atcgtcaact tcgaccgcgc actgcgcacg cttttcgccg
     2461 gtgctagctc tgttcgccgg ttcccgggcg gggaactgaa tgaaagcaat ctagatgaca
     2521 aggacagacg caacgctgct gcgctaatgc gcgttaatca tgctggcgaa atttgtgcgc
     2581 aggcgctcta tcacggacag gcgctgacgg cgaggaatgc agaggtccaa caggcgctgg
     2641 agtcagcggc aatcgaggaa acggagcatt tggcctggac aatgcagcgc atcaccgaac
     2701 tcaatggcaa ggttagcgtg ctgaacccgg tgctttatgc aagttcgttt gcgatcggca
     2761 cggtatgtgg actgctgggc gacaagtgga atctcggctt tctggcggag acggagaaac
     2821 aggtggaagg acacctcgaa gggcatctcg accgcctgcc gcatgaggac aatagaagcc
     2881 gggcgatcct gctgcaaatg aaagaagacg aagctcaaca tgcccgcacc gcactgaggc
     2941 acggtgcggc cgaactgcca ttgccggccc gcaaaatgat gcgcgcggcc tccaaactca
     3001 tgacgcgcgt tacgtactgg gtctgagact aggcctccac aatcgtgaag tcgtgggtaa
     3061 ttgccgccgt cttgcccagc atgatggagg cagagcaata cttgtgcgcc gacaactcaa
     3121 ttgcccgctg cacattctcg ggcttgatat ttcgccctgt aatcgtgaat tggaagtgaa
     3181 tgcgggtaaa gacttttgga tcaataggcg cgcgatctgc atctatctca agcacacagt
     3241 cactgacgtc ctgccgtgct ttcttgagta tggtaacaac gtcatatgcg ctgcatccgc
     3301 cggcgcctgc cagcatggtc tccatagggc gcgggcccaa attgcgacca ccgccctccg
     3361 ccgctccgtc cattactatg gcatgactgc tttcggtttc accaacgaag gcgacatgct
     3421 cgatccattt cactcggact ttcattgagc tggcttccag atttgcgtaa cgggcgcaca
     3481 tggtaacagc catggttcac tgcgctgcac attcagcgtg ttcacgtcgg tcgtaggatc
     3541 cggtcgatcc acgaagtgcg gcgttttgct cactcgttat tccggttttt agaaatggag
     3601 gttttgtcac gcaagctcag ttttttacgc caaaacaaag agttgaaacg taattactgg
     3661 gattttggat ccgacttgcg ataaaatgcg cagttattca aaaaccagac aggctagagc
     3721 atggctgaca gtgatgtcga tcgtggcaaa agacgtttcc tgattgccac aacgagcgtg
     3781 gtgggcggca ttggcggggc tgccgctatc gttccattcg cgatgagtat ggctcccagt
     3841 gcgcgggcgc gggcggccgg
//
LOCUS       DATMZQ010000045         4858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_378636, whole genome shotgun sequence.
ACCESSION   DATMZQ010000045 DATMZQ010000000
VERSION     DATMZQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378636"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VN496_01595"
     CDS             complement(<1..538)
                     /locus_tag="VN496_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HXF15661.1"
                     /translation="MRQRVRTFYAGKKLRPGTKIKTEKKMLLAVCPRIRLFCAFFAML
                     GVTVRVACAADNPAAELEAPTVEVVGTTPLQGIGVPVNQVPSNVQTATGADIQRQRTL
                     DLTEYMENNLGSVTLNQGQNNPFQPDVQFRGLTASPLLGTPQGLSVFVDGVRVNEPFG
                     DVVNWDLIPQNAISTITLI"
     gene            648..2060
                     /locus_tag="VN496_01600"
     CDS             648..2060
                     /locus_tag="VN496_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXF15662.1"
                     /translation="MLGLATEYFACWPGVLPLYSGYVRMVFSFMNLFRNDRPTSAAIA
                     DRPSLRRDLLVLAAVPLLTFALSSAFQLNEWIGSLTGPREAYQADELPLTLLALAVGL
                     AWFSWRRSRQMMEQVALRLDSEEQYRMLFTENLAANLLASLDGRIKLANPAAVRLLDF
                     ASPDELNGRLLQEFYVDRALWVTHRDALRAGRQIELPALQLTRRDGAVVHAAAKLSAR
                     LSPAREPELHIYLADMTDVTAMQGQLANALQENRRLSQRSMQVQEEERRNLARELHDE
                     LGQSLNAIKVDAVTIRDGATNVAEVQRSAKGIIEVSGQVYDVVRSLMRRLRPVALDEL
                     GLRSAVEYGIEQWRRRHPAVRCTFVTEGELDDLGEQMNITLYRLAQECLTNVAKHAQA
                     TEVAISLVRADGDDPGCEVVRFSFEDNGHGFNPGLRSDGLGLIGLRERVEALGGRLDL
                     RSAPEQGARVCASIPLKGNS"
     gene            2057..2734
                     /locus_tag="VN496_01605"
     CDS             2057..2734
                     /locus_tag="VN496_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF15663.1"
                     /translation="MNESRFTPVRVVLVDDHAVVREGYKRLLERTQDITVIAEVSGGE
                     EAYRVVCEMQPEVTVMDINLGGMGGIEAVRRIIARLPDAKILMFSMHEDTVFSSRALQ
                     AGARGYVTKSAAPEVLVEAVRLVAQGKLYISHEMAQELALQMLPGRDNPIDALSAREF
                     EVFRLLVAGHALQEISKILCLSYKTVANYQSNIKHKLDVSNTAQVVRIAIAHGLIGSG
                     VEPEEPG"
     gene            2972..4396
                     /locus_tag="VN496_01610"
     CDS             2972..4396
                     /locus_tag="VN496_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXF15664.1"
                     /translation="MNRTLVLNVVGMTPALLVHAPHLNALAKQGTVRSLSTVTPAVTT
                     TVQSTFVTGTLPREHGIVGNGWYFRDLAEIWLWRQSNHLVGGEKLWDAARKRDPSFTC
                     AKLFWWYNMYSSADIAVTPRPMYPADGRKLPDIYTQPPELRDELQHALGQFPLFRFWG
                     PAADIVSTRWIAQSALHVLQKNGPTLTLVYLPHLDYNLQRLGPNHPDIARDVAQVDAI
                     CGELIDVAQRAGMHVVALSEYGITDVSQPVHINRALRSAGWLRVRDELGRDQLDAGAS
                     DAFAVADHQIAHVYVARRELLGEVASLLRSLPGVERVLDEQGKREIGLDHPRSGELVA
                     IARPDSWFTYYHFLDDERAPDYARTVDIHRKPGYDPVELLLDPAQRLVKARIGWKLVQ
                     KKLGMRYLMDVIALDAGLVKGSHGRPTDDPAEGPLFITSAAHLVGDGPVAATDVKRLL
                     LDHVFNARATNKERMHAEQVKEQV"
     gene            4393..>4858
                     /locus_tag="VN496_01615"
     CDS             4393..>4858
                     /locus_tag="VN496_01615"
                     /inference="COORDINATES: protein motif:HMM:NF035938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HXF15665.1"
                     /translation="MNPASLLKRWLESRLPAEGRAWLFGVAEKLQGSSDADLYRSISL
                     VTRKIGKADLLLNAEELQQASAARAGWDPSDWSLDQAARIYLLLVSASNGATFLRRLD
                     QLCNTADVGELVAFYRGLPLYPDQPRYVLRAAEGIRTNMKAVFEAVAHRNPYP"
BASE COUNT          950 a         1398 c         1528 g          982 t
ORIGIN      
        1 gaataagcgt gatcgtcgag attgcatttt gcggtatcaa gtcccagttc accacatcgc
       61 cgaacggttc gttgacccgc acaccatcga caaacaccga caggccctgc ggcgtgccga
      121 gcaaggggga agcggtaagg ccgcggaatt ggacatccgg ctggaatggg ttgttttgcc
      181 cctgattgag cgtaacgctg cccagattgt tttccatata ctcggtcagg tcgagcgtcc
      241 tctgcctttg aatatcggct ccggttgcag tttgcacgtt ggacggcacc tggttcacgg
      301 gaacgccgat gccctggagt ggcgtggtgc cgacgacttc gaccgtgggc gcttccagct
      361 cggccgccgg attgtcggcg gcgcacgcga ctctgaccgt gacgccgagc atcgcaaaaa
      421 atgcacagaa cagccgtatt cgagggcaaa cagccaaaag catttttttc tccgttttta
      481 tttttgtgcc ggggcgcagc ttctttccgg cgtaaaacgt tctgacgcgc tggcgcaagc
      541 acgtcagcaa tctaagtgcc atgccatagg tttggtcagg gagcggctca catgcacatc
      601 gggaaatatt cctgactcaa caaatcggcc agtccgatgc tagacagatg ctaggacttg
      661 cgacggaata tttcgcttgt tggcccggag tgcttccgct atatagcggg tatgtccgca
      721 tggtattttc cttcatgaat ttgtttcgta acgaccggcc aacgtctgcg gccattgcag
      781 accggccttc actgcgtcga gatctgctcg tgctcgccgc cgttccgctg ctaaccttcg
      841 cgttgtcgag cgcgtttcag cttaatgaat ggattgggtc gttgaccggg ccgcgcgagg
      901 cttaccaggc cgatgagctt ccgctgacgc tgctggccct ggccgtgggc ctcgcatggt
      961 tctcgtggcg tcgctcgcgg cagatgatgg aacaggtggc gttgcgcctg gacagcgaag
     1021 agcaatatcg gatgcttttc acggaaaacc ttgcagccaa cctgctggca tcccttgacg
     1081 gccgcataaa actggccaac cccgcggccg tgcgcctgct ggatttcgcc agtccagacg
     1141 aactgaacgg acggctgctg caggaatttt atgtggaccg cgcactctgg gtgacgcatc
     1201 gcgatgcgtt gcgggcaggc cgccaaatcg aattgccagc gttgcagctt acgcggcgtg
     1261 atggggcggt ggtgcacgcg gctgccaaat tatcggcaag gctatcgccg gcgcgcgagc
     1321 ccgagttgca catttacctg gccgatatga ccgatgtcac cgccatgcag ggccagctcg
     1381 caaacgcgct acaagaaaac cggcgcttgt cgcaacgttc gatgcaggtg caggaggaag
     1441 aacggcgaaa tctcgcgcgc gaactgcacg atgaactggg ccagagcctg aatgccatca
     1501 aggttgatgc cgtaacgata cgcgacggcg ccacgaacgt ggcagaggtg cagcgtagtg
     1561 ccaagggcat cattgaagta tctggccagg tatacgatgt ggtgcgcagt ctgatgcgac
     1621 gcctgcgacc ggtggccctg gacgaactcg gcctgcgatc ggccgttgaa tacggcatcg
     1681 aacagtggcg gcgcaggcac cccgcggtac gctgcacctt tgttaccgaa ggggaattgg
     1741 acgaccttgg cgagcagatg aacatcacgt tgtacaggct cgcgcaggaa tgcctgacca
     1801 acgtggccaa gcatgcgcaa gcgaccgagg tggccatttc gctggtgcgt gccgatggtg
     1861 atgaccctgg ctgcgaggta gttcgcttca gcttcgaaga taacggccat ggcttcaatc
     1921 cgggactgcg ctcagacgga ctggggctga tcggcttgcg tgagcgggtg gaagcgctgg
     1981 gtggccggct tgatttgcgc agtgcgccgg aacaaggggc ccgcgtttgc gcctcgatcc
     2041 ccttgaaagg gaattcatga atgaatcacg gtttacgccg gtgcgcgtcg tgctggtcga
     2101 cgatcacgcc gtggtgcgcg agggctacaa gcggctgctg gagcgtacgc aggatattac
     2161 ggtaatcgct gaagtctccg gcggcgagga agcctaccgc gtggtctgcg agatgcagcc
     2221 tgaggtcaca gtcatggaca tcaacctcgg cggcatgggc ggcatcgaag cggtccggcg
     2281 gatcattgca cgcctgccgg acgcaaaaat cctgatgttc agcatgcacg aggataccgt
     2341 tttctcttcg cgcgcactgc aggctggcgc gcgcggttat gtgaccaaat ccgctgctcc
     2401 agaggtactg gtggaagcgg tgcgcctggt cgcacaaggc aagctctaca tcagtcatga
     2461 gatggcgcag gaactggcgt tgcagatgct gcctggccgt gacaacccaa tcgatgcgtt
     2521 gtcggcgcgc gaattcgaag tgtttcgcct actggtggcg ggccacgcct tgcaggaaat
     2581 ctcgaagatc ctgtgccttt cttataagac ggtcgcaaac tatcagtcca acatcaagca
     2641 caagctcgat gtcagcaata ccgcgcaagt ggtgcgaatt gccatcgccc acggcttgat
     2701 cggcagcggc gtagagccag aggaacctgg ttgacggggc ggtatcctcg ccgcgcgaag
     2761 gatcccattg gtgcgccgca atgccgggcc ggattcccga aattcaggcc tcgcgttaaa
     2821 attgccatcg tagctgcaca ctcaatgttg cctctgcagc gtaagcaagg gacgtaaaca
     2881 ggcggccggg ccgccacaag gtccgcatgc gacggatcgg cgccatacgg agggacgttg
     2941 tgaatacagc aagtcagctg gcggggcgaa catgaatcgc acgctggtgc tgaacgtcgt
     3001 tggcatgacg cctgcgctgc tggtccatgc accgcatctc aacgccctgg caaagcaggg
     3061 aaccgtacgc agcttgagca cggtcacccc cgccgttacc acgacggtgc aatcgacctt
     3121 tgttaccgga acgctgccgc gtgagcatgg catcgttggc aacggttggt attttcggga
     3181 tttggctgag atttggctgt ggcgccaatc gaatcatctg gtgggcggcg aaaagctgtg
     3241 ggatgctgcg cgcaagcgcg atccgtcgtt cacctgcgcc aagctgttct ggtggtacaa
     3301 catgtattcg tcggcggaca tcgccgtcac gcctcgcccg atgtatccgg cggatggacg
     3361 caagctgccc gatatctata cccagccgcc ggaattgcgc gacgaattgc agcacgcgct
     3421 cggccagttt cccctctttc ggttctgggg cccggcagct gatatcgttt cgacgcgctg
     3481 gatagcgcag tcggccctgc acgtgctgca gaaaaacggg ccgaccctga cgctggtcta
     3541 tctgccccat cttgactaca acctgcagcg tctgggaccc aaccatcctg acattgcgcg
     3601 tgacgtggcg caggtggatg caatctgcgg tgaactgatc gacgtggcgc aacgagccgg
     3661 catgcacgtc gtcgcgttat ccgaatacgg catcaccgat gtgtcgcagc cggtgcatat
     3721 caaccgggcg ctgcgcagtg ccggctggtt gcgcgtgcgc gacgagcttg gccgagacca
     3781 attggatgcg ggcgcttcgg atgccttcgc ggtggcagat caccagatcg cccacgttta
     3841 tgtggcacgc cgtgaactgc tcggcgaagt cgccagcctg ctgcgctcgc tgcctggcgt
     3901 cgagcgtgtt ctcgacgagc agggcaagcg cgaaattggg ctggatcatc cgcgttccgg
     3961 cgaattagta gccattgcga ggccggacag ctggttcacc tactatcatt ttctggacga
     4021 cgagcgcgcc ccggactatg cgcgcaccgt ggacattcat cgcaaaccgg gttatgaccc
     4081 cgtcgaattg ttactcgatc ccgcgcagcg ccttgtgaag gcgcgcatcg gctggaaact
     4141 ggtgcagaaa aaactcggca tgcgctatct aatggatgtg attgctctcg atgccggcct
     4201 ggtgaagggt tcgcatggcc ggcccaccga cgatcccgcg gaagggccgt tattcatcac
     4261 gtcagcggcg cacttggtcg gggacggtcc ggtagcggcc accgacgtca agcgcctgct
     4321 gcttgatcat gttttcaacg cgcgcgccac gaacaaagag cgtatgcatg ctgaacaggt
     4381 gaaggagcag gtatgaatcc ggcctcgttg ctgaagcgct ggctggagtc gaggctcccc
     4441 gcggaaggcc gcgcgtggtt gttcggcgta gccgaaaaac tgcagggcag cagcgatgcg
     4501 gatctgtacc gatccatcag cctggtgact cgcaagatcg gcaaggcgga tctcctgctc
     4561 aacgccgagg agttgcagca ggcgtcagcc gcacgtgcgg gctgggatcc ctcagactgg
     4621 agcctggacc aggccgcgcg tatttatctt ctgctcgtct cagcatcgaa cggtgcgacg
     4681 tttctgcgtc gcctcgatca actctgcaat accgcagacg tcggtgaact ggtcgcgttc
     4741 tatcgcggat taccgttgta tccggatcag ccgcgctatg tgctgcgtgc ggccgaaggc
     4801 atacgcacca acatgaaagc ggtgttcgaa gcggttgcac atcgaaatcc gtatccgt
//
LOCUS       DATMZQ010000046         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2796206, whole genome shotgun sequence.
ACCESSION   DATMZQ010000046 DATMZQ010000000
VERSION     DATMZQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796206"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VN496_01620"
     CDS             <1..478
                     /locus_tag="VN496_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HXF15666.1"
                     /translation="VGFPQWADRFPFIGAAIWLALLVTVPLRQPAWNKLPDAVRGSIF
                     LLSLVLSASLMPVEALPPASWQTALGLGYISAVFDNIPLTALALHQGGHDWGVLAYAV
                     GFGGSMIWFGSSAGVALSNLFMESKSVVAWLRYGWHVVVAYPLGFFVLLWLMGWQP"
     gene            620..1600
                     /locus_tag="VN496_01625"
     CDS             620..1600
                     /locus_tag="VN496_01625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HXF15667.1"
                     /translation="MKQKILVTREVFDETIEVLREHFEVEANQADRIYTSDELATKLQ
                     GKNGVQTQSSDRIDAQLLDKCPGLKAVCNTAVGYNNIDIEACTRHGVMVTNTPGVLTD
                     SVADYAIGMIIATCRRMTEGEAYLRKGEWKGTFLKQMLGQDVHGATLGLFGFGRIGQA
                     IARRARGFDMKIVYHSRNRAAPDAEAEAGAIYAGKEDLLRQADVVLLILPYTPETQHF
                     IAARELSLMKRSAVLVNMARGGIVDDAALIDALKRKTIWAAGLDVYENEPKVNPGLLQ
                     LANVVLSPHIASASEPTRKAMAMTAARNLVAALSGKTPPNLLNPDYRTFL"
     gene            1647..>2195
                     /locus_tag="VN496_01630"
     CDS             1647..>2195
                     /locus_tag="VN496_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF15668.1"
                     /translation="MTELYRLGLREAALRIRAGKLTSLQYTRDLIAQIDHLEEDVQAW
                     QWLDRSRALDLAARADKSDSALRVAHPLHGIPIGVKDLFYTAGIPTEMGCRAYVGYVP
                     DISADVVVRLESAGGIMFGKTVTTEAAFMVPAKTRNPWNTNHTPGGSSSGSAAAVAAG
                     FVPGALGTQTNGSVIRPAAFCGV"
BASE COUNT          463 a          588 c          664 g          480 t
ORIGIN      
        1 tgtcggcttc ccgcaatggg cggaccgatt tccgttcatc ggggccgcga tctggctcgc
       61 attgctggtg acggtgcctc tccggcaacc ggcgtggaat aagctgccgg atgcggtccg
      121 cggaagcata tttctgcttt cgctggtgct cagcgcttcg cttatgccag tggaagcgtt
      181 gcccccggca tcttggcaga cggcgctggg cctgggctat atttcggcgg tattcgataa
      241 tattccgctg accgcgcttg cattgcatca gggtggacat gattggggcg tgctggctta
      301 tgcggttggc tttggaggat cgatgatatg gttcggctct tcggccggag tggccctgtc
      361 gaacctgttc atggaatcca aatcagtcgt cgcgtggctg aggtacggct ggcacgtggt
      421 cgtggcctac cccttgggtt tctttgtttt attgtggctg atgggatggc aaccctgaat
      481 ttccccggtg ttggaatttt gtccggacgt agaaaccccg aaacggcccg ccgatccacc
      541 cgaattcttg cccctactga cagcttcgcg ctaaccttcg cgtgcgtatg gcataaccaa
      601 gaacaatgag aaaaatagaa tgaagcaaaa gatccttgtg acacgggaag tttttgatga
      661 aaccatcgaa gttctcaggg agcatttcga ggtcgaggcc aaccaggcgg acaggattta
      721 taccagcgat gaattggcca cgaaacttca gggcaagaac ggcgtgcaga cccaatccag
      781 cgatcggatc gacgctcagt tactggacaa gtgtccgggc cttaaggccg tctgcaatac
      841 cgcggtcggc tacaacaata tcgacatcga agcctgtacc cgtcacggtg tcatggtgac
      901 gaatacgccg ggggtgttga cggatagtgt tgctgactat gcaatcggga tgattatcgc
      961 gacctgcagg cgcatgaccg aaggtgaggc ttatctgcga aaaggcgaat ggaaaggtac
     1021 ttttctgaaa caaatgcttg gccaggacgt gcatggtgca accttgggcc tctttggctt
     1081 cggacgtatc ggtcaggcaa tcgcgcggcg agcgcgcggc ttcgacatga aaatcgttta
     1141 ccacagccgc aatagggcag ccccggacgc cgaagctgag gcaggcgcaa tctatgccgg
     1201 caaagaggat ttgctgcggc aggcggacgt ggtcttgttg atccttccct acacgccgga
     1261 aacccagcac ttcattgctg cacgcgaact gtcgctgatg aagcgttctg cagtactggt
     1321 caatatggcg cgggggggaa tcgtcgacga tgcagcgctg attgatgcgc taaagagaaa
     1381 gacgatttgg gccgccgggc tggatgtcta cgagaacgaa cctaaagtaa atccgggatt
     1441 gctacagctg gccaatgtcg tactgtcacc gcatattgcc agtgcatcgg aaccaacgcg
     1501 caaggccatg gcaatgaccg ccgccagaaa tctggttgcg gcgctgtccg gcaaaacgcc
     1561 gccaaattta ttgaatcccg actacagaac ttttctctaa gcgcaatacc ttgcaacgcc
     1621 gccttggcgg cgttcggaac tccatcatga cggaacttta cagactggga ttgcgtgaag
     1681 ccgcgctgcg tatccgcgcc ggaaagctga ccagcctgca atatactcgc gaccttattg
     1741 cacagatcga tcatctggaa gaagacgtgc aggcttggca gtggctcgac cggtcccgcg
     1801 cactcgatct ggccgcgcga gcggataagt cagacagtgc cttgcgggta gcgcacccgt
     1861 tgcacggcat tccgatcggc gtcaaggatt tgttttatac ggccggtatc ccgaccgaga
     1921 tgggttgtcg cgcctatgtc ggatacgtac cggacatcag cgccgacgtg gtcgtgcggc
     1981 tcgagtccgc gggcggaatc atgttcggca agacggtgac gaccgaagct gcgttcatgg
     2041 tgccggctaa aacgcgcaat ccctggaata ccaatcacac gcctggcggt tcttccagcg
     2101 gatcagcggc agccgtggct gcgggatttg ttccgggtgc acttggtacg cagaccaacg
     2161 gttcggttat tagaccagcc gcgttttgcg gcgtc
//
LOCUS       DATMZQ010000047         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2452984, whole genome shotgun sequence.
ACCESSION   DATMZQ010000047 DATMZQ010000000
VERSION     DATMZQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452984"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1979)
                     /locus_tag="VN496_01635"
     CDS             complement(90..1979)
                     /locus_tag="VN496_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HXF15669.1"
                     /translation="MVRGSRIINPRSALAFLHDVVVAGLAWLAAFWFRFNFDIPPEYL
                     LIMSTTVGWVVALDAVCFWMFGLYRGLWRYASIHDLRLIVVAVSVAAIAFPAVVILIQ
                     PGFVVPRSVYVLHPMLLLFAMGGSRLLYRAWKERHIGKLGAIHGKPVVILGAGDAAVA
                     LLKDLSGSAEWRVIGLLDDDPTKQKRQILGATVLGVITELPALVPRFDIEHAILAMPD
                     ETHDVRRRAIGVCQGAGIEVLTVPSFDDLLSGKVTVSQIRHVELDDLLGRDPVKLDSA
                     GLKELLTDKTVMVTGAGGSIGAELCRQIARYHPSRLVLFELSEFALYQIEQEFHGALP
                     RLALICMVGDAKNPQRVAQALDKYRPQVVFHAAAYKHVPLMERENAWEAVRNNVYGTY
                     VVASEAKRHGVEKFVLISTDKAVNPANVMGASKRLAEMVCQALHGPQGTRFIMVRFGN
                     VLGSAGSVIPKFREQIAAGGPVTVTHPDITRFFMSIPEAAQLVLQAGAMGRGGEIFVL
                     DMGEAVRIVDLAKDMIRLSGFAEEDVKIAFTGLRPGEKLYEEVLAESEEALTTPHPKL
                     RIARAREVPDGWLEALTAWLQQDSSPEDGKVRSALGQWVPEYDSLRHGVDPVIRLGRK
                     EEIGR"
     gene            complement(1972..>2760)
                     /locus_tag="VN496_01640"
     CDS             complement(1972..>2760)
                     /locus_tag="VN496_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HXF15670.1"
                     /translation="LHASPGFLAAVAALAIASYFDDRHALPASVRLAIHLLAAALFLW
                     LASTSTGTTMTIVLLLAIGWITNLYNFMDGSDGLAGGMAVIGFGTYGLAAWLAGSSEF
                     ALLAWSIAAASAAFLFFNFPPAKIFMGDAGSIPLGFLAGALGVSGWLQKLWPLWFPLV
                     VFAPFIADASTTLVRRLLRGERVWQAHREHYYQRLILSGWSHRRAALYEYALMVLCGV
                     VALISLRQPLVGQFILLAALALTMALAMWAVDRRWRRFSAEVHG"
BASE COUNT          640 a          865 c          774 g          481 t
ORIGIN      
        1 cgactttcac tgacactggt accttttaaa gaagtcatcc aggcgctgcc gtcgcagcac
       61 gcatcacaaa tgggaaatcc ctgccgggcc tatcgtccta tttcttcctt gcgaccgagc
      121 cgtatcactg gatccacgcc atgccgaagc gagtcgtact ccggcaccca ttgccccaga
      181 gccgatcgga ccttgccatc ctccggcgac gaatcttgtt gcaaccacgc cgtcagtgcc
      241 tcgagccagc catcgggaac ctcacgtgca cgcgcaattc gaagcttggg atgcggggtg
      301 gtcagggctt cctcgctctc ggcaaggact tcttcataga gcttctcccc ggggcgcaag
      361 ccggtaaacg ctatctttac gtcttcctcc gcaaacccgg acaggcggat catgtccttg
      421 gccagatcga cgatacgcac tgcctcgccc atgtcgagca cgaaaatttc gccaccgcga
      481 cccatggcgc ccgcttgcag cacaagttgc gctgcttccg ggatggacat gaaaaaacgc
      541 gtgatatcgg ggtgcgtcac ggtgaccggt ccgcctgcgg cgatttgctc gcggaatttc
      601 ggaatcacac tgcccgcact gccgagcacg ttgccgaaac gaaccatgat gaatcgcgtt
      661 ccctgcggcc cgtgcaaagc ctggcagacc atttccgcga gccgcttgct cgcgcccatc
      721 acattcgcgg ggttgacagc cttgtcggtg gaaatcagaa cgaacttctc aacgccgtgg
      781 cgcttggcct cggaggccac aacgtacgtt ccatagacgt tgttgcgcac cgcttcccag
      841 gcattttccc gttccatcaa aggcacatgc ttatatgccg cggcgtgaaa aacgacttgg
      901 ggccggtatt tgtccagggc ttgtgcgaca cgctgtggat tcttggcgtc accgaccatg
      961 cagatcagtg caaggcgcgg caaggcgccg tggaattcct gctcgatctg gtagagcgca
     1021 aactccgaca actcgaaaag caccaaacgg ctgggatgat aacgcgcgat ctgccggcac
     1081 aattcggcgc cgatggaacc gcccgcgccg gtaaccatca cggtcttgtc agtcaagagt
     1141 tccttcagac cggcgctatc gagtttgacc gggtcgcgtc cgagcaggtc gtccagttcg
     1201 acatgtcgaa tctgggaaac ggtgactttg ccgctcaaca ggtcgtcgaa cgacggcacg
     1261 gtcagtacct cgatgccggc accctggcag acaccaatcg cccgtcggcg aacgtcatgg
     1321 gtctcgtccg gcatggcaag aattgcgtgc tcaatgtcaa accgcggcac aagcgcaggc
     1381 agctccgtta taacgcccaa taccgtagcg ccgagtatct gccgcttctg tttggtcggg
     1441 tcgtcatcca gcagaccgat gacgcgccac tccgcactgc cggacagatc tttgagcaag
     1501 gctactgccg cgtctcctgc gccaaggatc actactggct tgccgtgaat cgcgccgagc
     1561 tttcctatat gacgttcctt ccacgcccgg tagagaagcc ggctgccccc catggcgaat
     1621 aacagcagca ttggatggag cacgtaaacc gatcgcggca cgacgaagcc gggctgaatc
     1681 agaatgacga ccgcgggaaa ggcgatcgct gcgacgctga cggcaactac gatgaggcgc
     1741 aggtcgtgaa tgctggcata ccgccagagt cctcgataca acccgaacat ccagaaacaa
     1801 acagcgtcga gtgccacgac ccagccgacg gtggtgctca ttatcaacaa gtactctggc
     1861 ggaatgtcga agttgaagcg aaaccagaaa gcggccagcc acgccaggcc agcaacaacg
     1921 acgtcatgga gaaaagcgag cgctgaacgc ggattgatga tccgcgagcc cctaaccatg
     1981 cacttccgcc gagaatcttc tccaccgcct gtcgacggcc cacatcgcca aggccatggt
     2041 cagcgcaaga gccgccagca atataaattg cccgaccagc ggctgccgca ggctaataag
     2101 cgccacgacc ccgcagagaa ccatgagcgc atattcgtac aacgcagcgc ggcgatggct
     2161 ccagccggaa agaataaggc gctgatagta gtgctcacga tgagcctgcc acacacgctc
     2221 gccacgcaac agccgtcgaa ccagggtcgt actggcatcg gcaatgaaag gtgcgaatac
     2281 gaccagggga aaccacagcg gccagagttt ctgcagccag ccagaaacgc ccaatgctcc
     2341 agccagaaag cccaaaggta tcgatccggc gtcgcccatg aagattttcg ccggaggaaa
     2401 attgaagaaa aggaaggctg ccgaagcagc cgcaattgac catgccagca gcgcgaactc
     2461 gctgcttccg gcgagccagg ccgccagtcc gtatgtcccg aaaccgatga cggccatacc
     2521 ccccgcgagc ccgtcggaac catccatgaa gttataaaga ttcgtgatcc agccgatcgc
     2581 gagcagtaga acgatagtca ttgtcgtccc ggtagatgtg cttgccagcc acaagaacag
     2641 tgcagcggcg agcaggtgga tcgcaagtct gacggaagcc ggtaaggcat gtcggtcgtc
     2701 gaagtaagac gcaatagcca gcgcagcaac ggcggccagg aagccgggac tggcatgcaa
//
LOCUS       DATMZQ010000048         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3142863, whole genome shotgun sequence.
ACCESSION   DATMZQ010000048 DATMZQ010000000
VERSION     DATMZQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3142863"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..>2100
                     /locus_tag="VN496_01645"
     CDS             180..>2100
                     /locus_tag="VN496_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXF15671.1"
                     /translation="MLRSLWLLGLTALLLRLAIGFFNLLRIERNAIQILDGEWPQLVH
                     QLSRQVGLARRVTLLFSKQSVMPMTWGSIWPRIMLSLEADAWSAERKRAVLLHELAHV
                     KRRDCLSQWVVRMACAIYWFNPLVWLANARIHTERERACDDVALSAGHDAADYAEHLL
                     AMASWPRAGYSLNVTALAMARPSKLESRLRAILDSRCVRRSVSWKFTLAALSLLVATL
                     LPLAAIRSVAQDRGPRTAEAEKQGAQPESNATRISEFDSSASALANTANRKRHRRLAA
                     KATMFNRDYATTLAQLRTAAAGPDADFDDWIWLGHALQLHGNWRQAVDAYKKALKELA
                     PRVLPVIEEAPDRNLEQKHYREQWAELVLLIGRLELEELGDKASAVETWSTSLRFFEC
                     FTRPIRELATESAVQLATRERCDEATPTKLYFALMSPLASQRELALLYEHEGRISEAL
                     ECWTRVRLGRLVHQAAMADVDPLKVAALYAKQEPSGPLLQLPIFLTCTPEKPRMMLRP
                     ESGESRLAWWGNHEWNRFGIAPPPGFSFSALKLTCKVKQNASGTPSQLVCSLAGRRQQ
                     PLSSIQLLNRAWDVARGEGEYTDTLDVAVPYAADLLFVEFGGRGSQTSVSEIQMDATL
                     RSTGDLPQLPPAPARI"
BASE COUNT          398 a          632 c          647 g          423 t
ORIGIN      
        1 tgccgcatga ctcgccagat acatcgcacc gtcctccggc ggcgagccca cacttggatc
       61 ggccggcagc ttccgggcca gagtccgcca aagcggtgac cgtccctgca cctgcggtcg
      121 cggcgccgag ggtgttggcc caaccgtcgt cgacggcgcc gcgatactcg ttcgatcaaa
      181 tgctccgatc cctatggcta ttgggtctca cggcccttct gttgcgattg gcaatcgggt
      241 ttttcaactt gttgcggatc gagcgcaatg ccatccagat tctggacggc gaatggcccc
      301 agctcgtcca tcagttgagc cgacaagttg gcctggcgcg tagggtgacc ttgcttttca
      361 gcaaacagtc cgtcatgccg atgacctggg gcagtatatg gccccgaatc atgctctcgc
      421 tcgaggccga cgcctggtct gccgagcgca agcgggctgt tttgctccac gaattggcgc
      481 acgtgaaacg ccgcgattgt ctatcccaat gggtcgttcg catggcctgc gccatctatt
      541 ggttcaatcc gctggtctgg ctggccaacg ctcggatcca cacggagcgt gaacgcgcct
      601 gcgacgatgt ggcactttct gccggtcacg acgcggccga ttatgcggag cacttgcttg
      661 ccatggccag ctggccgcgg gctggttact cattgaatgt gactgccttg gccatggctc
      721 gcccctcgaa gctggaaagt cgacttcgcg cgatactcga ttctcgctgc gttcgacgat
      781 cggtgtcgtg gaagttcacg cttgcggccc tgtcattact cgtggcgact ctgttgcccc
      841 tggcagcaat tcgcagcgtc gcacaagatc gaggtccgcg gaccgcagag gcggaaaaac
      901 agggagccca gccggaaagc aatgcgacca ggatttccga attcgacagc agtgcatcag
      961 cgctcgcgaa tacggcgaat agaaagcgtc atcggcgcct cgcggccaag gccaccatgt
     1021 tcaaccgtga ctacgctacg acgctagccc aacttcgtac ggctgccgca ggccccgatg
     1081 cggactttga cgattggatt tggctcgggc atgcgttgca acttcatggc aattggcggc
     1141 aggccgtcga tgcctacaag aaagcgttga aggagctcgc gccacgggtc cttcccgtga
     1201 tcgaggaggc gccggatcgc aatctcgaac agaaacatta ccgcgagcaa tgggcagagc
     1261 tcgtcttact gatcggccgc ctggaactgg aagagctagg cgataaagca tccgcggttg
     1321 aaacctggtc cacgagcctg cggttcttcg agtgcttcac tcggcccatc cgtgagctgg
     1381 ctaccgaaag cgccgtgcag ttagcaactc gagagagatg cgacgaggcc acgcctacga
     1441 aactgtattt tgcgttgatg agcccgcttg cgtcccagcg ggagctggcc ctgctctacg
     1501 aacacgaggg ccggatttct gaagccttgg agtgctggac ccgtgttcgt ctgggcaggt
     1561 tagtacacca ggctgcgatg gccgatgtcg acccgctcaa ggtggcggcg ctctatgcga
     1621 aacaggagcc gtcaggccct ttgctccagc tcccgatctt cctgacgtgc acgcctgaaa
     1681 agccgcgcat gatgttgcgg cccgagagtg gcgaaagccg attggcctgg tgggggaacc
     1741 atgaatggaa ccggtttggg atagctcccc cgcccggatt ctcgttttca gcgctgaaac
     1801 tgacctgcaa ggtcaagcag aacgcgagcg gaacccccag ccaactggtt tgctcgctgg
     1861 ccggccgccg acagcagcct ttgtcgagca ttcagttgct caaccgtgcg tgggatgttg
     1921 cgcgcggcga gggcgagtac accgatacgc ttgatgtggc ggttccttac gcagccgacc
     1981 tgttgtttgt tgaatttggc ggaaggggaa gtcagacaag cgtcagcgag attcaaatgg
     2041 atgccacact gcggtcgacg ggcgatctgc cgcagctacc cccggcgccg gcgcgaatta
//
LOCUS       DATMZQ010000049        10825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2799150, whole genome shotgun sequence.
ACCESSION   DATMZQ010000049 DATMZQ010000000
VERSION     DATMZQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2799150"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1450
                     /gene="earP"
                     /locus_tag="VN496_01650"
     CDS             329..1450
                     /gene="earP"
                     /locus_tag="VN496_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HXF15672.1"
                     /translation="MRWDLFCAVVDNLGDIGVCWRLARQLAPEQGADVRLWVDDLATF
                     RQLAPALDTGAARQRLGDIEVCAWTPVFPEVEPADIIVETFGCELPASYVQAMKQRAK
                     APVWINLEYLSAEPWVGRYHGLPSPQPPLVKYFFFPGFAPDTGGVLVERGLNAERAAF
                     QADAAAQARFLAGIGIAPRNPRESFVSLFCYPQAPTAALFDAWASGSERIRVIAFADT
                     PSAQALVGNGLANGRMRGSLQGQIVPFLSQEDYDRMLWICDWNFVRGEDSFMRAQWAA
                     RPFVWQLYPQQEQSHRIKMQAFLARYLGASVMGSAAGLVDLWTAWNGLLPPARVAPAW
                     TASMATAGGLSRLAQMWPEQLARLGDLAGNLARFCVERV"
     gene            1491..2048
                     /gene="efp"
                     /locus_tag="VN496_01655"
     CDS             1491..2048
                     /gene="efp"
                     /locus_tag="VN496_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXF15673.1"
                     /translation="MILAQEVRAGNVIMVGKDPMVVQRAEYNKGGRNAAVMKMKLKNL
                     LTGGGAEVVYKADEKLENVILERKEAVYSYFSDPMYVFMDSEYNQLEVEKDNMGDALN
                     YLEDGLPCEIVFYDDKPISVTLPNSVVREITTEPAVRGDTSGKVLKPAKIGSGFVVHV
                     PLFCESGDKIEIDTRTGEYRNRVKG"
     gene            complement(2160..2372)
                     /locus_tag="VN496_01660"
     CDS             complement(2160..2372)
                     /locus_tag="VN496_01660"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HXF15674.1"
                     /translation="MGLDQRGSPVFCVVSDAAGQWQVKEEGFEKALASFDQRSDALKY
                     ARDLADTKEGSQVKAYDEQGREVPGA"
     gene            complement(2403..3047)
                     /locus_tag="VN496_01665"
     CDS             complement(2403..3047)
                     /locus_tag="VN496_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXF15675.1"
                     /translation="MSIRKFIVAVGGALLAAGAWTGAMAQTTTPDSVSSALGGWDDAA
                     GTRPGSWLPYTSYGYVGGGIGRSKYDVSCAPGFSCDDSDVGYKIFTGGKVSRIVGVEV
                     GYVYLGKAAANGGSQKAQGINFSVIGNLPIGDKFNVYAKVGGIWGWTSTSTSPLSGQG
                     GGDDHGLNWSYGLGVQFDVTKNWAVQGDWDHYRFDYSNQSANAQLYSVNLVYKF"
     gene            3423..4583
                     /locus_tag="VN496_01670"
     CDS             3423..4583
                     /locus_tag="VN496_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXF15676.1"
                     /translation="MMEKKTAQPRGVDPSRYGPRDSAREPAITVLPQAPRPKPPRSRP
                     DWAFVGLFILAALYAFHFARALLLPITLAVLFSLLLSPIVSWLRKWRVPEPAGAAVVL
                     VALLSGIVTAFYSLAEPAAAWLEKAPESLTVAEDKVRKFLQPVTQVQRAADKIEAITR
                     TTSSKKPREVVVQAPGFGVWITSQTPYLITGTVSTLVLLYFLLASGDLFLRKTVKLIP
                     TLRNKIHAVEVGNEVQRELGRYFVTVSLINAGLGIATAIVMKALGMPNPLLWGALAML
                     LNYIPYLGPTVCLGVLSMAALMSFDDFSHIWPVPAAFVTLVVVEGQLVQPFIVGRSLS
                     LNPVMVFSAFLVLGWLWGIAGMFVAVPILVTLKVVCDHVERFAAMGEYLGRN"
     gene            4675..6057
                     /locus_tag="VN496_01675"
     CDS             4675..6057
                     /locus_tag="VN496_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXF15677.1"
                     /translation="MPHALLVDDDADTLESLEELIAREGFTVATATSLTGAREQMVLQ
                     RPDVVLLDLVLPDGDGMDLFQDVESRATTEVVMITGHASIETSIEALRLGAADYLIKP
                     VNIKQLKAILSRVARPTDLKKEISALRGELRSLGRFGRLLGSSTAMQGVYDQIQRVAP
                     TVATVLITGESGTGKELVAQTLHDLSRRRKNPFIPVNCGAISPQLIESEVFGHEKGSF
                     TGAVREHKGYFERANGGTLFLDEITEMPVELQVKLLRVLEEGTFMRIGSDREIEIDVR
                     IVAATNRVPEEAVGEGKLREDLLYRLQVFPLHLPPLRERGEDVKLIAGQFLKDLNQAD
                     GTAKYFAPEAEDLLMEWHWPGNVRELRNVVQRAFIMADDAITAACLPLETGKARKSNT
                     GPFLQVRVGSTVAEVERALTLATLQQCGGVKEKAAQMLGVSLKTLYNRLREYDSQREP
                     AASESASHPH"
     gene            6070..6390
                     /locus_tag="VN496_01680"
     CDS             6070..6390
                     /locus_tag="VN496_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15678.1"
                     /translation="MPGWRLLWPNRRSRFAVAPYAELDQTDFQVVFHAGRSHYSEFDW
                     RSFVHNIEIDAVVPGDNFARQLHDMFAGDLRESARIDADTWRRCCLGVRIKEVSARLW
                     EYWL"
     gene            6606..6803
                     /locus_tag="VN496_01685"
     CDS             6606..6803
                     /locus_tag="VN496_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXF15679.1"
                     /translation="MNWDRIEGNWKQFKGKVKEKWGKLTDDELDKIAGRRDQLAGKIQ
                     QSYGISRDEADRQVQDWEARL"
     gene            complement(7035..10049)
                     /locus_tag="VN496_01690"
     CDS             complement(7035..10049)
                     /locus_tag="VN496_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXF15680.1"
                     /translation="MESDTRRYLWGPALVLLIALAATAAVVWQLLRMADSEDLSRFQR
                     HVSGMQLAVEDQFDSYIGLLREGAALFTADRTPGAAQFHNFVARLNRKNEYPGPQNRR
                     PQSPSDVRYPGILGMGWLQVDDAMNPLPRASRGQRVLLADTNGVETLGFIFLEPPEWR
                     TPAMASFEMFTDPGSRAAMERARDTGKPAMSGKVTVIRDFWAKKRSGFHVFHPVYNSG
                     GVPPARVRRQALRGFLVVSVQVDEMLSALQTEEASRGIDMQVYDGTQTAPAALLYSSD
                     TAHPAEDTGYRPRFSTTALMDGPGKSWAMVFYTRPEFELASNRNMAPQVLLIGVLVSL
                     LLAGINLVQRRASAALNASEVRYRRLFEASPDGVFLFDAHTGRISSVNPALAQLLGRT
                     PEQLVGKFLWEIGLFGDDASGRAAFHELQGKDYHRFQDLSVVTPGGQRHDVEFTCNAY
                     MANDMRFMQCNVRDITARRLAENALRDSEERYRSLVEISPQGVWIIDRAGLPLFINRY
                     WVDYSGVDVTRGRSGAWTEQVHPEDRPRMIEVWKRARMEDSLYEFELRMRRASDGAYR
                     WHLARGVPVHDAHRRIDKWLGVFIDINERKQAEEGRVQQLQREYVLRTQAEAANRAKD
                     DFLATLSHELRAPLNAILGWTQTLQQGDADSETVRRALSQIDASANAQARLINDLLNV
                     ADIGSGRLRLDIQIVDLLALVDAIVESLRPAITARDITLKTIIDPDAGHLSGDPARLQ
                     QIIWNLLSNAVKFTPHGGHITLSLALVGSQIEIAVSDDGEGIGAEFLPYVFDPFRQAD
                     PSIKRRHGGLGLGLAIVRHLTELHGGTVSAQSAGENKGSRFLVRLPLRPPQRAIPQAA
                     PQPPLPPTSVAPRASPKRRLTGLRILSVDDDENTREMLQEALERAGAVVESAASAQEA
                     LDKLASFAPDVLLSDIGLPDEDGYDLMRKVRALNAERGGGTPAVALTGYAREQDQKSA
                     LAAGYQGFVAKPVNLDELANAILAASAMHDPA"
     gene            10374..10532
                     /locus_tag="VN496_01695"
     CDS             10374..10532
                     /locus_tag="VN496_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HXF15681.1"
                     /translation="MSIGTLLLIVVILLLIGAVPSWPYSRNWGYGPSGILGVVVLIVV
                     LLVVMGRI"
     gene            10573..>10825
                     /locus_tag="VN496_01700"
     CDS             10573..>10825
                     /locus_tag="VN496_01700"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXF15682.1"
                     /translation="MQPVNRFFKVMLSAVVLFAAMGCANHAHDKQATSQRADDSWITS
                     KVKTAFVKDKTLSASDINVETNNGSVALSGFVNDPGDVSY"
BASE COUNT         2232 a         3085 c         3203 g         2305 t
ORIGIN      
        1 atcaccggcc aggtcatttc catcaatggg ggaatggcgt aaaggccgcg aaggccgcaa
       61 aggccgcaaa ggccgtgaat atcgtgaatg ccgtgaatgg cgtagggccg cgtcgcgtta
      121 ttcacgacga cgtccaccgc tctacgcagt taaagtattt ccaattttca cggctcaacg
      181 cagttaacgc ctttccgata ttcacgtatt cacgatattc acggctcttt taaaagcctt
      241 tgcgacattc acgtattcac gatattcacg gttcaacgta gttacggcct ttccgacatt
      301 cacgatattc acggcattca cggctcttat gcgttgggat cttttctgtg ccgtggtgga
      361 caatcttggc gacatcggcg tctgctggcg gctggcacgc cagcttgcac ccgaacaggg
      421 cgctgatgtg cgattgtggg tggacgatct cgcgacgttc cgccagcttg cacccgcgct
      481 tgacaccgga gcggcccgac aaaggctggg cgatatcgaa gtgtgcgcgt ggacccctgt
      541 ttttcccgaa gtcgaacctg ccgacatcat agtagagaca tttggctgcg aactgccagc
      601 gtcctatgtt caggcaatga agcaacgggc aaaggccccg gtgtggatca atctcgagta
      661 cctgagtgcc gagccgtggg tcgggcgtta ccatggcttg ccatcgccac aacccccgct
      721 ggtcaaatat tttttctttc ctggatttgc accagatact ggcggtgtgc tcgtggagcg
      781 cggcctgaat gcagaacgcg ctgccttcca ggccgatgcc gcagcgcagg cgcgctttct
      841 cgccggtatt ggtatcgccc cgcgcaatcc acgcgaatcc tttgtctcgc tgttctgtta
      901 tccgcaggca ccgacggccg ctttattcga tgcatgggct tctggctccg agcgcatccg
      961 ggtaattgcg ttcgccgata ccccgtcggc ccaagccctg gttggcaatg gactcgcgaa
     1021 cggtcgtatg cgtggttcgc tgcaaggcca gattgttccg ttcctgtcgc aggaagatta
     1081 cgaccgcatg ttgtggatct gtgactggaa tttcgtacgc ggtgaagatt ccttcatgcg
     1141 cgcgcaatgg gccgcgcgtc cttttgtatg gcagctgtat ccgcagcagg agcagtctca
     1201 tcgtatcaag atgcaggctt ttcttgctcg ttacctcgga gcttccgtga tgggctccgc
     1261 ggccggattg gtggatctgt ggaccgcatg gaatggtctg ctgccgccgg cccgcgtggc
     1321 gcccgcctgg acagcgagca tggcgactgc gggaggtctt tcgcgcctgg cacagatgtg
     1381 gccggagcaa ctggcacggc tcggcgatct ggccggaaac ctggccaggt tttgtgtgga
     1441 gcgagtataa ttccgcgctt tttccccact taaatttcag gaattgcaac atgatactgg
     1501 ctcaggaagt gcgcgccggc aacgtaatca tggtcggaaa ggatccgatg gtcgtgcagc
     1561 gcgctgaata caacaagggc ggccgcaacg ctgccgtcat gaaaatgaag ttgaagaatc
     1621 tgctaaccgg tggcggggcc gaggtggtct acaaggcgga cgaaaaactc gaaaatgtca
     1681 tcctcgagcg caaggaagcg gtgtattcgt atttttccga tcccatgtat gtgttcatgg
     1741 acagcgaata taaccagctc gaagtcgaga aggacaatat gggcgacgcg ctcaattatc
     1801 ttgaggacgg actaccgtgc gagattgttt tctacgacga caaaccaatt tcggtaaccc
     1861 tgccgaactc ggtggtgcgc gagatcacga ccgaacccgc cgtgcgcggc gatacttcag
     1921 gcaaggttct caagcccgca aagatcggta gtggtttcgt cgtgcatgta ccattgttct
     1981 gtgaatcggg agacaagatc gaaatcgaca cccgcaccgg cgaataccga aaccgggtga
     2041 agggctaagt cgcaacccaa taaaccgctc gaggtaaaaa gaaaaggcgt gaaaccatgc
     2101 atggtcacgc cttttcattg ccgatgcccg gatgccgcgt taccgggaca ctgaactact
     2161 taggcgcccg gtacttcgcg gccctgttcg tcgtaagcct taacctgcga accttccttg
     2221 gtgtccgcaa gatcgcgcgc gtatttcagc gcgtcgctac gttgatcgaa cgaagcgagc
     2281 gctttttcaa acccttcctc tttcacctgc cactgcccgg cagcgtcgct gacgacgcag
     2341 aataccggtg aaccacgctg atccaagccc atgccattcc tcctgcgtgc gatgccgacg
     2401 ctctagaatt tgtagaccag gttgaccgaa tacagctggg cgtttgcact ctggttggaa
     2461 taatcaaaac ggtagtggtc ccagtctcct tgtacagccc agttcttggt cacatcgaac
     2521 tgaaccccga ggccataact ccaattcagc ccgtggtcgt ctccgccccc ctggcccgat
     2581 agggggctgg tgctggtact tgtccagccc cagattccac cgaccttggc atacacattg
     2641 aatttatcgc cgatcggcaa atttccgatc acgctgaagt tgatgccctg ggctttttgg
     2701 ctgccgccat tggccgccgc cttgcccaga tacacatagc caacttcaac gcctacgatg
     2761 cgcgagacct ttcctccggt aaatatctta tagccgacgt ccgaatcatc gcagctaaac
     2821 cctggcgcgc agctgacatc gtatttcgac cgcccaatgc cgccgccgac ataaccgtag
     2881 gaagtatacg gaagccagga acccggtctg gtcccggctg catcgtccca tcctccgagc
     2941 gccgaactga cggaatctgg ggtggtagtt tgtgccatgg ccccagtcca tgctcccgcc
     3001 gcaagcaggg cgccaccgac tgccacaata aatttacgaa tagacatcac atgaactcct
     3061 tatatgtttc gcgggaatga ctcgttggtt ttcctcgctg cgcggacggc tgcaaggtgt
     3121 atgccatggc tttgtggcgg acgtattgag ggccgtcgca atcgcccggt gtgtgccgga
     3181 aggactagat cggcgatgac tgctgtcaac accggtacgg tttactgcgg aactgtaata
     3241 ttttccggaa cagactggag ccagcggttg tctggcaggt aatacgtccg aaaggatgcg
     3301 tgcggcgcct gggctactct ggcgccggcc ggaccacgcg cggaattgcg gcggcatcgg
     3361 acagtccaac gctctcaacg tgcgggcgcg cgcccgtact ggctaaatgg gatttctgga
     3421 aaatgatgga aaagaaaacc gcgcaaccac gcggcgttga cccgtcgcgc tatggaccac
     3481 gcgacagcgc ccgcgaaccg gccatcacgg tgctaccgca agcgccccgg ccaaaaccgc
     3541 cgcgcagcag acccgattgg gcgttcgtcg ggctttttat cctggcggcc ctgtatgcat
     3601 tccattttgc gcgcgcattg cttcttccca taacgctggc ggtgctattc agtcttctcc
     3661 tttcccccat cgtgagctgg ttgcgcaaat ggcgcgttcc ggagccggcg ggcgccgcgg
     3721 tggtcctggt tgcactgttg agcggcattg tcacggcgtt ctatagcttg gccgagcccg
     3781 ccgccgcctg gctcgagaag gcaccagaaa gcttgacggt tgccgaagac aaagtccgta
     3841 agttcctgca gccggtcacg caagtgcagc gcgcagcgga taagatcgag gccattactc
     3901 gaaccaccag ctccaaaaaa ccgcgcgaag ttgtggtgca agcccccgga ttcggcgtct
     3961 ggatcaccag ccagacgcct tatttgatta ccggaacggt ctctacgctg gtgctgttgt
     4021 acttcctgct tgcttccggc gatttgttct tacgcaaaac ggtaaagtta attccgactt
     4081 tgcggaacaa gatccatgcg gtcgaggtcg gaaacgaggt ccagagggaa cttggccgat
     4141 atttcgttac ggtcagcctc atcaatgcag gtttgggcat cgcaactgcc atcgtcatga
     4201 aggcactcgg catgccaaac ccgttgctgt ggggcgcttt ggccatgcta ttgaactaca
     4261 ttccttatct gggtccaacg gtgtgcctgg gtgttctgag catggcggca ctgatgagct
     4321 tcgatgactt cagtcacata tggccggtac ccgcggcttt cgtcacgctc gtggtcgtgg
     4381 agggacagct ggtgcagcct ttcattgtgg gacgcagcct cagcttgaac cccgtcatgg
     4441 tgttcagtgc atttctggtg ctgggatggc tatggggaat cgctggaatg ttcgttgccg
     4501 tgccgatcct ggtcacgctc aaagtagtgt gtgaccacgt cgagcgtttt gctgccatgg
     4561 gggagtacct gggcagaaac taaatttcgc agaaccacgg aaacaataac tgcacggagc
     4621 cgcggagacg tccgcagtgg acggttcggc tcatgaacaa ggatcagaca aaccatgccg
     4681 catgcactgt tagtcgatga cgatgccgat actctggaga gcctggagga gttgattgcg
     4741 cgcgaaggat ttacagtagc caccgccacc agtctgaccg gagcgcgaga gcagatggtg
     4801 ctgcagcggc cggacgttgt attgctggac ctcgtactgc ccgatggcga tggcatggac
     4861 ctgtttcagg acgtcgagtc tcgcgcgacc acggaggtgg tcatgattac cggtcacgca
     4921 agcatcgaaa cttcaatcga ggcgctgcgg cttggcgctg cggattacct catcaagccg
     4981 gtcaatatca agcagctgaa ggcgattctg tcgcgtgtgg cgcgtcccac ggatctgaag
     5041 aaggagatca gcgcgctgcg gggcgagttg cgcagtctag gacgatttgg tcgcctgctt
     5101 ggttcatcga ccgcgatgca aggcgtttac gaccagatcc aaagagtcgc gcccacggta
     5161 gcaacggtac tcattactgg agaaagcggc acggggaagg aactggtggc gcagacgctg
     5221 cacgacttga gccgccgccg caagaacccg ttcatcccgg ttaactgcgg ggcgatttcc
     5281 ccgcagctta tcgaaagtga agtgttcggc catgaaaagg gcagctttac cggcgctgtg
     5341 cgcgagcaca agggctattt cgaacgcgcc aacggcggca cgctgtttct ggacgagata
     5401 accgagatgc cggttgaact ccaggtcaag cttctgcgcg tgctggagga aggaaccttc
     5461 atgcggatcg gctcggaccg tgaaatcgag attgacgtgc gcatcgtcgc ggcaaccaac
     5521 cgtgtgcccg aggaagcggt aggcgaaggc aagttgcggg aggatttgct gtaccggctg
     5581 caagtttttc cgctgcactt gcccccgctg cgcgaacgcg gagaggatgt gaaactgatc
     5641 gccgggcagt tcctgaaaga tctcaaccag gccgacggta cggcaaaata ttttgcaccc
     5701 gaagccgagg atctgctgat ggagtggcat tggcctggca atgtgcgcga attgcgcaac
     5761 gtcgtgcagc gcgcgttcat catggccgac gatgcgatca ccgcggcttg cttgccgctc
     5821 gaaacaggca aggcgcgcaa gagcaacacc gggccgtttt tgcaggttcg cgtgggcagt
     5881 accgttgccg aagtcgagcg cgcgttgacg cttgcgacgc tacaacagtg cggcggtgtg
     5941 aaagaaaagg cggcgcaaat gctcggcgta agtctgaaga cgctgtacaa ccggctgcgc
     6001 gaatatgatt cccagcgcga gccggccgct tccgaaagcg cctcccatcc ccattgaaca
     6061 aggcgcggca tgcctggttg gcgccttctc tggccgaacc ggaggagccg gttcgcagtc
     6121 gcgccgtacg ccgaacttga ccagacggac ttccaggtcg tattccacgc cggacgctcg
     6181 cattattccg agttcgattg gcgcagcttc gtgcacaaca tcgaaatcga tgccgtggtg
     6241 cctggcgata attttgcgcg gcaactgcat gacatgtttg cgggcgatct tcgcgaatcc
     6301 gcgcgcattg acgcagatac ctggcgacgc tgttgtctcg gcgtgcgtat caaggaggta
     6361 tctgcgcgct tgtgggagta ctggctgtaa ttgcatctcc acgtatcgtg gtgcagtgga
     6421 atattttcgc aggctgtcgg tatttattac cgaccagcgc ggttaaaaat ttttaaaagg
     6481 atgattcttc gaaacaaaga acttggcaca ggggttgcaa cacaattcag cgagcagtgc
     6541 gtcgatgaag cgtttcagtt gatcgatgtg ttactcggtt ttatcaaacg cagaaggagc
     6601 ttgccatgaa ctgggatcgt attgagggca actggaagca attcaaaggg aaggtaaagg
     6661 aaaagtgggg aaagctaacc gatgatgagc tagacaaaat tgccgggcgt cgcgaccagc
     6721 ttgctggaaa aatccagcaa tcatatggca taagccgtga cgaagcagat cgccaggtac
     6781 aggattggga agccaggctc tgaactgaaa cgatggagaa tgaatgcgat gcggtgacgg
     6841 tgtgaaagcg aagcagatgg ccagtaccgt cacgaacgat cggacagccg gagcggacac
     6901 ctccgcgtgg aggtgacgct ccccggtccc gtgaaccaag gttctgcggt caggaagtgc
     6961 cgcaggtaaa cagacccccg ttagccctcg ttccgcaagg accgggggct ctttttgtgt
     7021 cggacgcgtt gcattcaagc cgggtcatgc attgcgctgg cagccaatat cgcattcgct
     7081 aattcgtcga gattcacggg cttggcaacg aacccctggt agcctgcagc gagcgcggac
     7141 ttctgatcct gctcgcgcgc atagccggtc agtgcaactg ctggcgtgcc gcctccgcgc
     7201 tcagcgttga gcgcgcgcac ctttcgcatc aaatcatagc cgtcctcgtc cggcagcccg
     7261 atgtcggaca gaagaacatc cggtgcaaac gatgccagct tgtcgagcgc ttcttgcgcg
     7321 gaagccgcag attccactac cgccccggcg cgttcgagtg cctcctgcag catttcgcgg
     7381 gtattttcat catcatctac cgacagaatg cgtaaccccg tgaggcggcg ttttggactt
     7441 gcgcgcggtg cgacgctggt cggtggcagt ggcggttggg gtgctgcctg cggaattgca
     7501 cgctggggcg gacgcaaggg aaggcgcacc agaaaacgcg agcctttgtt ttcgccagcg
     7561 ctttgtgcgg atacagtgcc gccgtgcagt tcagtgagat ggcgaacgat cgcaagtccg
     7621 aggcccagcc cgccgtgccg gcgtttgata ctcgggtcgg cctgcctgaa tggatcaaaa
     7681 acatagggaa ggaattccgc gccgatcccc tcaccgtcgt cgctcaccgc aatttcgatc
     7741 tgcgatccaa ccagcgcgag gctcagcgtg atgtggccgc cgtgaggtgt gaatttcacg
     7801 gcattggaca gcaggttcca gatgatttgc tgcagccttg caggatctcc ggacaggtgg
     7861 ccggcatccg gatcgatgat cgtcttgagc gtgatatccc gagcggtaat ggcgggacga
     7921 agcgattcga caatggcatc gacaagtgcc agcagatcca ctatttgaat atcgagccgc
     7981 aaccggccgg aacctatatc ggcaacattc agaaggtcgt ttatgagcct ggcttgcgca
     8041 ttggcgctcg catcgatttg cgatagcgcg cgccgcacgg tctcgctatc ggcgtcgcct
     8101 tgctggagcg tttgcgtcca gccaagaatt gcgttcaaag gcgcccgcag ctcgtgcgaa
     8161 agcgtcgcca gaaaatcgtc cttcgcccgg ttcgcagctt ctgcctgagt gcgcaaaacg
     8221 tattcgcgct gcaactgctg cacgcgtcct tcctcagcct gtttgcgctc gttgatgtca
     8281 atgaatacgc ccagccattt gtctatgcga cgatgcgcat cgtggaccgg tacgccgcgc
     8341 gccaggtgcc agcggtaagc gccgtctgaa gcgcgccgca tgcgcagctc gaactcgtac
     8401 agggaatctt ccatgcgggc gcgcttccag acttcgatca ttctcggccg atcttccgga
     8461 tgcacctgct ccgtccacgc gccggatctg ccgcgcgtta cgtcaacgcc gctatagtcg
     8521 acccaatagc ggttgataaa cagcggcagg ccggcccgat cgataatcca aacgccctgc
     8581 ggagagatct ccaccagaga gcgatagcgt tcctcgctgt cgcgtaatgc gttctcggct
     8641 agcctgcgtg cagtaatgtc tcgaacatta cattgcatga agcgcatgtc gttggccatg
     8701 tacgcattgc aggtgaactc cacatcgtgc cgctgcccgc ccggcgtaac gacggataga
     8761 tcctgaaagc ggtggtaatc cttgccctgc aattcatgga aagccgcgcg cccgctcgcg
     8821 tcatcgccga ataatccgat ttcccacagg aactttccca ccaattgctc gggcgtgcgg
     8881 ccgagcaact gggcaagggc gggattgacg ctgctgatcc gcccagtgtg tgcgtcgaac
     8941 aggaatactc cgtcaggcga agcttcaaag agcctgcggt agcgcacttc cgacgcattc
     9001 agcgcggcgc tcgctcgcct ctgaaccagg ttgatgccgg ccagcagcag gctgaccagc
     9061 acgccgatca gcaaaacctg tggggccatg ttgcgattcg aagctaactc gaactccggt
     9121 ctggtgtaga acaccattgc ccaggatttt cccgggccat ccatcagcgc tgtcgttgaa
     9181 aaacgcggcc ggtagccggt gtcttccgcg ggatgcgccg tgtcagaact gtagagcagc
     9241 gccgcaggtg cggtttgcgt cccgtcgtag acctgcatgt cgatgccccg actggcctct
     9301 tccgtttgca gagcagacag catctcatcg acctgcacgg atacgaccag aaatccgcgc
     9361 agtgcctggc ggcgcacgcg tgccggcggc acgccaccgc tgttatatac cgggtgaaaa
     9421 acatggaagc cgcttcgctt cttggcccaa aaatcgcgga tcaccgtgac ttttccggac
     9481 atcgccggct tgccggtgtc acgcgctctc tccatggcag cgcgactgcc agggtctgtg
     9541 aacatctcaa agctcgccat tgccggcgtg cgccattccg gcggttcgag gaagataaat
     9601 cccaatgttt ccacgccgtt cgtatctgct agcaacaccc gctgtccgcg cgacgcacgc
     9661 ggaagcggat tcatcgcatc gtcgacttgc agccaaccca tacccaggat tcccgggtag
     9721 cggacgtccg atggagactg gggtcgccga ttctgcgggc cgggatattc gtttttacgg
     9781 ttcagccgcg cgacaaaatt atgaaactgc gcagcgcccg gtgtgcggtc tgccgtaaac
     9841 agggcggccc cctcgcgcag cagtccgatg tagctgtcga actggtcttc gacggccagc
     9901 tgcatgcccg atacgtgcct ctggaaacga ctgaggtctt cggaatcggc catgcgcagc
     9961 aattgccaca cgaccgcggc agtagcagca agcgcaatca gtaataccag cgccggtccc
    10021 caaaggtagc gtcgagtgtc ggattccatc ctggactgtg cagcaaaacg ctgcgttatg
    10081 acgacggcgt tcttacccgc ctgaccaata gaccgtcacg cagtcgctta gtgcgttatc
    10141 tcggcggcca cagcgtccct tggcgggaaa ttttacaaag agccgctgta atgtttaccg
    10201 gagcggtcgc tgtttcagcg ctccgggcgt gctgttgtct tttgttagtt gctgaattta
    10261 ttctccattc agccaaacag cgcaatggca tagaagctgc tgacttgggt ctacgcacgc
    10321 gtcacggcgt cagtggatcg acggcgagcg gacccagatc gaaaggaaac gccatgtcga
    10381 taggaacgct gttgttgatt gtcgtcattc tgttgcttat cggcgcggtt cccagttggc
    10441 cctacagcag gaactgggga tacggaccaa gcggcattct gggcgtggtc gtgctgatcg
    10501 tggtgctgtt ggtcgttatg ggacggatct gaagcgggcg caccaaatcg cacaacaagt
    10561 tggagatcga tcatgcagcc ggtcaatcgt ttcttcaagg tgatgctttc cgccgttgtc
    10621 ctgtttgcgg cgatgggctg cgcgaatcat gcccatgaca aacaggccac gagtcagcgc
    10681 gcagacgact cgtggatcac ttccaaggtg aagaccgcct ttgtcaaaga taaaacattg
    10741 agcgcttcgg acatcaatgt ggaaaccaac aatgggtcag tggcgctgag tggcttcgtc
    10801 aatgatccgg gcgatgtgag ctatg
//
LOCUS       DATMZQ010000050         7005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3836356, whole genome shotgun sequence.
ACCESSION   DATMZQ010000050 DATMZQ010000000
VERSION     DATMZQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836356"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..691
                     /locus_tag="VN496_01705"
     CDS             335..691
                     /locus_tag="VN496_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15683.1"
                     /translation="MDWYCVRIPGRRSAAAWMELAREVHRDAGFPHGCRVHHEISVRG
                     EHFLYFSPECLEVFPELLKLFGATTCNKPSGLDAFMQVLKWPRPPLSQSSSEVIRDPV
                     MRPPIPASQEQFLPGA"
     gene            1167..1412
                     /locus_tag="VN496_01710"
     CDS             1167..1412
                     /locus_tag="VN496_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15684.1"
                     /translation="MEATHNRRRSESMKSRQSRERIKRWQKLAGTLARTNPSWNVVKI
                     ASRIQRSAAGRKRNGLLTYSIDFIARNIRGGGNELSI"
     gene            complement(1435..1626)
                     /locus_tag="VN496_01715"
     CDS             complement(1435..1626)
                     /locus_tag="VN496_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HXF15685.1"
                     /translation="MADESSERKPDDPGRINLHEEWELTYWVKLLGTNEQDLRSAVKA
                     VGPQSDAVRQHLGKRQSQY"
     gene            1792..2091
                     /locus_tag="VN496_01720"
     CDS             1792..2091
                     /locus_tag="VN496_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15686.1"
                     /translation="MLTINDCIALSGLSKEEILAIGEHEHVPEMIAAELGRYLVQTHT
                     GEHRIKAMIVDDIRVARERGDHKHLLKLKLCLRHFLEHHAGPEHRPKKFSEKLDP"
     gene            complement(2117..3145)
                     /locus_tag="VN496_01725"
     CDS             complement(2117..3145)
                     /locus_tag="VN496_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HXF15687.1"
                     /translation="MSQAGGGKSRIFHEMDFDKPGKQIGYLRVPQSRDGGAWSTIEVP
                     IAIVNGSAGPAVLFTGGVHGDEYEGQIAGTRLARRLDPQSLRGKVILLPAVDLPAALA
                     GRRMCPIDGRDFNRCMPGDARGSFCQMLAHFIDTTIMPQVEVSVDVHAAGNAMEAALS
                     TTMHWADDAKTMQKTRTLAEQFAAPYNVIFWGVDEGGTVAAAAERHGALSISTEIGGY
                     GRVSVEGVRVAERGLDNVLKWMNMIEGKPDVSQPDGKRTMHMQVRDQKSYLFAPSDGL
                     FEPLYLPGQRVRAGERAGYLHFIEEWAREPLSMEFRIDGRVWMAPGSGRVRKGDVVAV
                     IMQEYQAT"
     gene            complement(3148..3939)
                     /locus_tag="VN496_01730"
     CDS             complement(3148..3939)
                     /locus_tag="VN496_01730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15688.1"
                     /translation="MTGATLSGVAVVTGGASGIGAACCRELAARGARVALVDRDQRVQ
                     SVARETGGRGWIADVADEAAIKHCADSIEAELGPVEILVNSAGVIQVPVPPDRLSMAN
                     WDEIVRVDQRGTYVAAMVFGTLMAGRRRGSIINIASVAGMRSMPLHAYAPAKAAVIAM
                     TECLAAEWGPAQVRVNAVSPGYTRTPALQDAIDKGERDVTALARNAALGRLVEPVEIA
                     RAVAFLASDQAAAITGINLPVDCGWLVAGSWHTYGGLRSLNKQES"
     gene            complement(4007..4723)
                     /locus_tag="VN496_01735"
     CDS             complement(4007..4723)
                     /locus_tag="VN496_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15689.1"
                     /translation="MSDRVMFEAQGIDVFYGTSQILFDVSVAVVEGQTMALLGRNGAG
                     KSTTLKAIAGIAPPARGRIRVSDKEVQGRKPYVIARAGIGFVPEDRQVFPQHSVEDNL
                     LIAAKKGPDGQDHWNLQRVYEMFPLLAPLKQRMAGYLSGGEQQMLTIARTLMGNPIGL
                     LLDEPSEGLAPVIVENIGDLLRRLHDMGVTVLLAEQNMNFCLGIASHASIIDKGQIVF
                     RDTIQNIKLNESVKSRYLAL"
     gene            complement(4720..5475)
                     /locus_tag="VN496_01740"
     CDS             complement(4720..5475)
                     /locus_tag="VN496_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15690.1"
                     /translation="MLTIEHLQKSFGGVTAINDVSLHFAPGSLTAVIGPNGAGKTSFF
                     NLVTGALRPDGGRIVLDGENIAGLSAPAIVRRGIGRAFQIASIFSSLTVREAILGAVN
                     AHRGWYAHVGRRFPLAQAQAQADEVMELVGLTATADTVSGNLSHGDQKVLDIALALAL
                     NPRVLLLDEPTAGMGPEERRKMMQTVHQLWQRKQMTLIFIEHDMDIVFGFAQTIRVLS
                     YGRVLAEGTPEQVRRHPEVIEAYLGRQYDAEHA"
     gene            complement(5477..6472)
                     /locus_tag="VN496_01745"
     CDS             complement(5477..6472)
                     /locus_tag="VN496_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF15691.1"
                     /translation="MRAPSMGRDVLIAIALLALLAVLPLVFPSKSFSDFVIRFSAFAI
                     FATSLNLLVGYAGMVSFGHGLFFGLGAYSFALMMQKLGVSIPLALALTVLINALVAAV
                     IGAICVPLKEIYFSFLTLAFQMLLYSLIIAWTAVTGGDQGLTGGVPRPPFLGVRLDDP
                     RHLYLFCCTMLVVSLFIMWHLVRSPFGYTLRMIRDNPRRASFLGIQVWRVRVLAFVVA
                     GIFGGVGGMLISLFVSSAYPDFAYWTISGEAVFMIMLGGISVFFGPLVGAALLLTLND
                     FITKVTEHYSLVLGIVILVFALGLRKGLLGFVVQAWHQYLPGARADKKAAPSRQH"
     gene            complement(6476..>7005)
                     /locus_tag="VN496_01750"
     CDS             complement(6476..>7005)
                     /locus_tag="VN496_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF15692.1"
                     /translation="WGAEFRSMGMPAAFQRAPVIVAGGVLPVFYLFLIGVTIVVALLL
                     GLLITRTRIGKIVRAAAVNPTMTSALGINTTLVYAGVFALGSLLAGLAGALAVPVRSL
                     VSGMGFSILIESFIVTVIGGMGSVAGALVGALLIGLVRSFGSIGFPLFTEGLMFLLMT
                     VILITKPTGLFGKEL"
BASE COUNT         1504 a         2134 c         2018 g         1349 t
ORIGIN      
        1 ttacgccgaa ggaacgcaag cgctccaggc tgcccggtca actgactaaa tcagttctgg
       61 ctaaagatcg ccacgcctcg atggcggccg gttttcttgt tctgcaatga tgttggcatg
      121 gtccccgaaa tcgttatacg ccgagacgca aagtgccaca aggcaccagg aaggcgcatc
      181 cgagcctatc tttgagcgcc acaactgatg gtggctcagt tgaataccgg ccccgcgtaa
      241 tcttcctctg acagcagtat cagataagta accgctgtca aactacggcg aggcggatat
      301 aaagcgactt caatctatta aaagggaggt cgccatggat tggtactgcg tccgtatccc
      361 aggcaggcgt tcggcggcgg catggatgga acttgcgcga gaagtgcatc gcgatgccgg
      421 ttttccccat ggctgtagag ttcaccatga gatctcggta agaggagaac actttctgta
      481 cttttcaccg gaatgtttgg aggtttttcc ggaactgctg aagctatttg gtgcgacgac
      541 gtgtaacaaa ccttctggcc tggacgcgtt catgcaagtg ctgaaatggc cccggccgcc
      601 gctgtcgcag tccagctccg aggtgatcag ggatcctgtc atgcgtccgc ccatcccggc
      661 ttcccaagaa cagtttttgc caggcgctta agcgagtcat cgtctatctt tcgatgtctt
      721 tggaagcaat ggccacagta tcgctgtgtg ccggtgaccg ggcgtcatcc tacattggag
      781 taatcgtaac cctacatcgg cgcgcgtggg cgcctataga atcccaggaa atttactctt
      841 agcgttgcag cgaaaataaa aacgacggtt gggcaggcgg ggaaacaggg cgggcgatgg
      901 catgcgatgg ttgcgtgcat agtccaaaga tcggcacgga ggatgttctg gggagcgctc
      961 cattccaaaa gcacagtcta gggattgcca tgcaaaccat gccaatggcg ttcaatattt
     1021 ttttacgcca ggcgcggcgc agcgatccaa ggtactaaag ttgccggcga gtcggaaact
     1081 catgcattga atggcataga agcgaacaac acagttaatc cgcttggttc agttcaacaa
     1141 gttatctcaa tgaggaggag gagagcgtgg aagcgaccca taacaggcgg cgttccgagt
     1201 cgatgaaaag ccggcaatcc cgggaacgca tcaagcgatg gcaaaaactt gcaggcacgc
     1261 ttgccaggac caatccctcc tggaatgtcg tgaagattgc aagccgcatt cagcggtccg
     1321 ccgcggggcg caagagaaac ggcctgctga cttattcgat cgattttatt gcccgcaaca
     1381 tacgcggtgg cggcaacgag ctttctatct gagcgcggct gatttaatag ccccctagta
     1441 ctgggactgt cgtttgccca gatgttgtcg cactgcgtcc gattgtggtc ccactgcctt
     1501 gacggcactc cgcagatcct gttcgttcgt gccgagtaac tttacccagt aggtcagttc
     1561 ccattcctca tggagattga tacggcccgg atcgtccggt ttgcgttctg atgattcatc
     1621 tgccatggca gcctcctttg gtgaccttcc aaaggttgat cggccgccac ggatttcgtt
     1681 ccccgccatt gccaagtggt ctgtaattga cccatatcaa gatttaatag ttgctggtgc
     1741 ggtacaagga acaatgcgac gctcagctgt cgccggatag caggagccat catgctgacg
     1801 atcaacgact gcatcgcgct gtccggcctg tcgaaagaag aaattctcgc gattggcgaa
     1861 cacgagcatg ttcccgagat gattgccgcg gaactcggac gctacctcgt gcagacgcac
     1921 acgggcgaac atcgtatcaa ggcgatgatc gtcgatgaca tccgcgttgc ccgggaacgc
     1981 ggggaccaca aacacctgtt gaaactgaag ctgtgtctgc gccattttct tgaacatcat
     2041 gcgggcccgg aacaccgccc gaagaaattt tccgagaagc tggatccctg acccgtgcag
     2101 tgctctgcat ggctcgctag gttgcctggt actcctgcat gatgacggcc accacatcgc
     2161 ctttgcgtac ccgtccggaa cccggagcca tccacacacg cccgtcgatg cggaattcca
     2221 tggacagcgg ttcgcgcgcc cattcttcga tgaagtgaag atagccggcg cgctctccgg
     2281 cgcgaacgcg ttgaccggga aggtacagcg gctcgaacag gccgtcgctc ggcgcaaaca
     2341 gataggactt ctggtcgcgc acttgcatgt gcatcgtgcg cttgccgtct ggctgcgaaa
     2401 cgtccggctt gccctcgatc atgttcatcc acttgagcac attgtcgagg ccgcgctcgg
     2461 ccacgcgcac accttccacg ctgacgcgcc cgtacccgcc gatttctgtt gaaatcgata
     2521 atgcgccatg gcgctcggcg gccgcggcca cggtaccgcc ttcatcgact ccccagaaga
     2581 tcacgttgta aggcgccgca aactgttcgg cgagcgtgcg cgtcttttgc attgtctttg
     2641 cgtcatccgc ccagtgcatg gtcgtgctga gcgctgcttc catggcgttc cccgctgcgt
     2701 gcacatccac tgaaacttcg acctgcggca tgatggtggt gtcaatgaag tgcgcgagca
     2761 tctggcagaa gctgccgcgt gcatcgccgg gcatgcagcg attgaagtca cgtccatcga
     2821 tcgggcacat cctgcgaccg gccagcgcgg cgggcaggtc cactgccggc aacaggatga
     2881 ccttcccgcg caggctttgc ggatcgagcc ggcgcgcgag ccgcgtccca gcaatctgcc
     2941 cttcgtattc atcaccatgc acgccgccgg tgaaaagcac tgccggcccc gcgctgccgt
     3001 tgacgatcgc gatcggaact tcaatggttg accaggcgcc gccatcgcgc gattgcggca
     3061 cgcgcaaata cccgatctgt ttgcctggct tgtcgaaatc catctcgtga aaaatacggc
     3121 tcttgccgcc gcccgcttgc gacatcgcta gctctcctgt ttgttgagtg agcgcaggcc
     3181 gccgtaggtg tgccaggaac cagcaaccag ccagccgcaa tccacgggaa gattgatgcc
     3241 cgttatcgcg gcggcctgat cgctcgcgag aaaagcgacc gcacgcgcga tctcgaccgg
     3301 ttcgaccagg cgtccaagcg ccgcattgcg cgccagcgcc gtgacgtcgc gctcaccctt
     3361 gtcgatcgca tcctgcaagg ctggcgtgcg tgtgtagcct ggagacactg cattcacgcg
     3421 gacttgtgcc ggaccccact ccgcggccag acactcggtc atggcaatga cggcagcctt
     3481 cgccggcgca tacgcatgca gcggcatgga acgcatgcct gcgacggaag cgatattgat
     3541 gatgcttccg cgccggcggc cagccatcag cgtgccaaat accatcgcgg ccacataggt
     3601 gccgcgctgg tcgacgcgca cgatttcgtc ccagttcgcc atggacaaac gatcgggagg
     3661 gacgggtacc tgaatcacgc cggcgctgtt taccagaatt tccactggac ccagctcggc
     3721 ctcgatggaa tccgcgcagt gttttatcgc tgcttcgtcc gccacgtcgg ctatccagcc
     3781 gcggccgccc gtttcgcgag ccactgactg gacccgttgg tctctatcca ccagcgctac
     3841 gcgcgcgccg cgtgccgcaa gttcgcggca acaagcggca ccgatgccgc tggcaccgcc
     3901 ggtgacgacg gccactccgg ataatgttgc gccggtcatc tattaccctc ccatttgcag
     3961 ctggcatggc tcattagcaa gtccaaaact gtcgcaaagc cgcgcatcac agcgcgaggt
     4021 agcggctttt cacggactcg ttgagcttga tgttttgtat cgtgtcgcga aaaacgattt
     4081 gtcctttgtc aatgatgctg gcgtggcttg cgatgccgag acagaagttc atgttctgct
     4141 cggccagcaa aaccgtgacg cccatgtcgt gtaatcggcg cagcaaatcg ccgatgtttt
     4201 ctacgatgac cggcgccagg ccctcgctcg gctcgtccag cagcagaccg atgggattgc
     4261 ccatgagcgt gcgcgcgatg gtcagcattt gttgctcgcc gcccgaaaga tacccggcca
     4321 tcctttgctt caggggcgca agcagcggga acatttcata aacacgctgc aaattccagt
     4381 gatcctgacc atccgggcct ttcttggctg cgatgagcag attgtcctcg accgaatgtt
     4441 gcggaaacac ctgccgatct tccggcacga aacctatgcc ggcacgcgca atgacataag
     4501 gctttctgcc ctgcacctct ttatcgctta cgcgaatgcg cccgcgcgcc ggtggcgcga
     4561 tgcccgcaat ggccttgagg gtggttgatt tgcctgcacc attgcggccg agcaatgcca
     4621 tggtctggcc ttcgaccacg gccacgctta catcgaacag gatctggctg gtgccataga
     4681 acacgtcgat gccctgcgct tcgaacatca cgcgatcgct catgcatgct ccgcatcgta
     4741 ttgccgcccg agataggcct cgatgacttc cggatggcgg cggacttgct ccggcgttcc
     4801 ctcggccagc acgcgcccgt agctgagcac ccggatggtt tgcgcaaagc cgaatacgat
     4861 atccatgtca tgctcgatga agatcaacgt catttgcttg cgttgccaca actgatggac
     4921 ggtttgcatc attttccggc gctcttccgg tcccatgccg gcagtgggct cgtcaagcag
     4981 cagcacccgc ggattcagcg cgagcgcgag cgcgatatca agcactttct gatctccatg
     5041 ggacaggttg cccgatacgg tgtctgccgt ggcggtcagc cccaccagct ccatgacttc
     5101 gtctgcctgc gcttgcgcct gcgccagggg gaagcgccgg cctacatgcg cgtaccatcc
     5161 gcgatgtgcg ttcactgcgc caaggattgc ttcacgcacg gtcagcgatg aaaaaatgct
     5221 ggcgatctga aaggcgcgcc cgatgccacg tcgcacgatc gcgggcgcgg aaagtccggc
     5281 gatattttcc ccgtcgagca cgatgcggcc gccatccgga cggagcgcac cggtcaccag
     5341 attgaaaaag ctggtcttcc ccgcaccgtt ggggccgata acggccgtca acgatcccgg
     5401 agcgaaatgc aggctgacgt cgttgatggc ggtcacgccg ccgaaagatt tttgcagatg
     5461 ctcgatcgtc agcatgtcag tgctggcgcg acggcgcagc cttcttgtcc gcccgcgcgc
     5521 cgggcaagta ttggtgccag gcctgcacca caaagcccag caatcccttg cgcagcccga
     5581 gcgcaaacac gagaatgaca atgccaagca ccaggctgta gtgctcggtc accttggtga
     5641 tgaaatcgtt caacgtcagc aatagcgcag cgcccaccag cggaccaaaa aaaacgctga
     5701 tgccgccgag catgatcatg aagaccgctt cgcccgatat ggtccagtat gcgaaatccg
     5761 gataagcgct cgatacgaac aggctgatca gcattccgcc cacgccgccg aatatgccag
     5821 cgacgacgaa ggccagcacg cgcacgcgcc agacctggat acccaaaaaa gatgcccggc
     5881 gtggattatc gcggatcatg cgcagcgtat agccgaatgg cgagcgcacg aggtgccaca
     5941 tgatgaacag cgatacgacc agcatcgtgc agcaaaacag gtataaatgg cgtggatcat
     6001 cgagccgcac gccaagaaag ggcggccgcg ggacgcctcc ggtgaggccc tgatcgccgc
     6061 cggtgaccgc ggtccacgca atgatgaggc tgtatagcag catctggaac gcgagcgtca
     6121 ggaatgaaaa gtagatttcc tttagcggca cgcagatggc gccaatcacg gccgccacca
     6181 gcgcattgat cagcacggtc aacgccaacg caagcggaat cgacaccccg agcttctgca
     6241 tcatgagggc aaagctgtac gcgcccagac cgaagaacag gccatggccg aacgaaacca
     6301 tgccggcata acccaccagc aaattgagcg acgtggcgaa aatggcaaaa gcggaaaagc
     6361 ggataacgaa gtcggaaaag gacttgcttg ggaacaccag aggcagcacc gccagcaatg
     6421 cgagcagggc gatcgcaatc aggacatctc gccccatcga cggcgcgcgc atgttttaga
     6481 gttccttccc gaacaggccg gttggcttgg tgatcaggat tacggtcatc agcagaaaca
     6541 tcagcccctc ggtgaagagc ggaaagccga tggagccgaa gctgcgcacc aggccgatca
     6601 acagcgcgcc gaccagtgcg ccggcgacgg agcccatgcc gccgatcacg gtgacgatga
     6661 atgattcgat caggatggaa aaccccatgc ccgagaccag agagcgaacc ggcacagcga
     6721 gcgcgcccgc tagcccggcc agcaaggagc cgagtgcgaa tacgccggca taaaccagag
     6781 ttgtattgat gcccagcgca gaagtcatcg tcggattgac tgctgccgcg cgcacgatct
     6841 tgccaatccg cgttctggta atcagcaatc ccagcagcag cgcgaccaca atggtgacgc
     6901 cgatcaggaa caggtagaag accggcaaca cgccacccgc cacgataacc ggcgcgcgct
     6961 ggaatgcagc cggcatgccc atcgagcgga attcagcgcc ccaga
//
LOCUS       DATMZQ010000051        16013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3836552, whole genome shotgun sequence.
ACCESSION   DATMZQ010000051 DATMZQ010000000
VERSION     DATMZQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836552"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            851..1966
                     /locus_tag="VN496_01755"
     CDS             851..1966
                     /locus_tag="VN496_01755"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HXF15693.1"
                     /translation="MPNTGGAGLQTAANVPPARLSGAGHAMSQDVTLRRVRVEDKRII
                     NGKADVNQLVPFKYKWAWEKYLSGCANHWMPQEINMSRDIATWKDPNGLTEDERLVVK
                     RNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLGLD
                     EGEIFNAYHEVSSIREKDEFLIPFIETLTNPEFRTGTPETDQQLLKSLIVFACIMEGL
                     FFYVGFAQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQIKMENPHLWTPRF
                     RDEIRDLMQQGVELEYRYAEDTMPRGVLGLNAPMFKEYLRYVANRRAQQIGLDPLYPG
                     ANNPFPWMSEMVDLKKEKNFFETRVIEYQTGGALNWD"
     gene            2394..2765
                     /locus_tag="VN496_01760"
     CDS             2394..2765
                     /locus_tag="VN496_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15694.1"
                     /translation="MAAKKKATKKTAKKSSAKKKPAKKAAKKSAKKPARKVAKKAAKR
                     PAKKAAKRPAKKKAAKKKAKKPAKKKAKKAAKKPAKKKAKKASKKKPAAKAPAAAAPA
                     APKPVSAPAAWPFPVAGNRPN"
     gene            complement(2988..3137)
                     /locus_tag="VN496_01765"
     CDS             complement(2988..3137)
                     /locus_tag="VN496_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15695.1"
                     /translation="MPRPTDNAGNAMLEWRFNRIYSKNAFPVGSGCRSEAKAEQSVIF
                     EALNL"
     gene            3177..3365
                     /locus_tag="VN496_01770"
     CDS             3177..3365
                     /locus_tag="VN496_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15696.1"
                     /translation="MKAPSGKIGLLCLVVFIIAMIGAFVPMGHVPYIGSGLTLVNHYH
                     EWLLMSGYALLLLAIYIL"
     gene            complement(3412..4053)
                     /locus_tag="VN496_01775"
     CDS             complement(3412..4053)
                     /locus_tag="VN496_01775"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXF15697.1"
                     /translation="MATLRLGDIAPDFTQESTQGTIKFHQWAGNNWVVLFSHPKDFTP
                     VCTTELGYTAKLKSEFDKRGVKAIAISVDPLDSHNKWIGDIEETQNTKLNFPILADAD
                     KKVSNLYDMIHPNANDTLTVRSVFIIDPNKKIRTTLTYPASTGRNFDEILRVIDSLQL
                     TDRYSVATPVNWKDGDDVVIVPSIQDPEVIKQKFPKGYKAVKPYLRITPQPNR"
     gene            4169..4384
                     /locus_tag="VN496_01780"
     CDS             4169..4384
                     /locus_tag="VN496_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HXF15698.1"
                     /translation="MDRKKVNSSSIRSVGYDERNRMLEVEFNDGRIMQYSGVSAELHR
                     RLMNAPSMVSYFRDNIEESFAAKRVKG"
     gene            complement(4411..5010)
                     /locus_tag="VN496_01785"
     CDS             complement(4411..5010)
                     /locus_tag="VN496_01785"
                     /inference="COORDINATES: protein motif:HMM:NF022609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HXF15699.1"
                     /translation="MDDIVIRAMGKWPDVPHVYGWLRLNRRGDWLIKGEHITNSGVTA
                     FIGRNYGVDERGRWFFQNGPQRVFVALDYTPFVVRTSGDGPPSLETHINCPIGKLSGA
                     WIDEMGSLLLRWGSEVGVLDDRDLPGILGLFTDLHGNAVDDGLLGRVFDREFRRQATG
                     IYIEYAGIRVPIGRIQAGEAAQKFGFDPAPQPANGEPEC"
     gene            complement(5011..5259)
                     /locus_tag="VN496_01790"
     CDS             complement(5011..5259)
                     /locus_tag="VN496_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15700.1"
                     /translation="MDDIVIRAMGKLLDVWPRLVSGTPGLGSPAGAAWGGVESSASVD
                     ASQYRVARVPADGRSANESRSRGSGHWSSGFAVAVQGK"
     gene            5447..6097
                     /locus_tag="VN496_01795"
     CDS             5447..6097
                     /locus_tag="VN496_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXF15701.1"
                     /translation="MALTLYYGSGSPYAWRVWLALEHKAIPYQLKTVSFSAGDLTKPE
                     FSALNPRHKVPTIVDDDFVLYESAAILEYLEEKYQSGKTLFPGNVRQRAVIRRLVREA
                     DQYLTHSMERVLDQVLFTEQDQWDLKEIAVGRADFLTELDLFESYLRGDWFGDGLSAA
                     DLTIYPMLALCLRMEKKKPDLGLTTSIPPKISLWMKRMQALPYYDKTYPPHWKANP"
     gene            6373..7641
                     /gene="otnK"
                     /locus_tag="VN496_01800"
     CDS             6373..7641
                     /gene="otnK"
                     /locus_tag="VN496_01800"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HXF15702.1"
                     /translation="MSILLGCVADDFTGATDLANTLVKAGMRTVQLMGVPRRTLTLPA
                     ADCIIVALKSRSNPASEAVAMSLAALDWLRERGAQQFYFKYCSTFDSTDKGNIGPVAD
                     AMLDALGADFTIFNPAFPTNKRTVYKGYLFVGDELLSESGMRHHPLTPMTDPSLVRVL
                     QRQTAHKVGLVQFSTIAKGVSATRSALEDLRKQGARHAVLDSLTDEHLMTLGESCADM
                     KLVTGGSGMAMGLPANFIRQGLLKGGQRAKLPKVTGPTAVIAGSCSMATQGQVALMSK
                     DHDAFELDPMAIASGRDVTRDALDWAAQRLSEKPILIYSTGTPEKVKAVQERLGREHA
                     GAVVEETFGKIAKGLVAKGVRRLLVAGGETSGAVVSALGVEGLAIGPEIDPGVPWTFS
                     IGEPSLALALKSGNFGTPDFFTKAFKVQPM"
     gene            7710..8342
                     /gene="otnC"
                     /locus_tag="VN496_01805"
     CDS             7710..8342
                     /gene="otnC"
                     /locus_tag="VN496_01805"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HXF15703.1"
                     /translation="MNETKLREEICMLGKSMFDRGLTVGSSGNISVRLNDGWLMTPTN
                     SCLGRLDPEALSKVDANGRLLSGGKQTKESFLHLAVYGERPSAGAIVHLHSTHSVAVS
                     CLAEIDPQQPIPPITAYYVMKIGNLMLIPYYAPGDMALANAVKEVAGKHHAILLANHG
                     PIVAGKDLESAVYATEELEETAKLYLLLRGDRLNILTQDQVAELNRKWPQ"
     gene            8515..8778
                     /locus_tag="VN496_01810"
     CDS             8515..8778
                     /locus_tag="VN496_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HXF15704.1"
                     /translation="MNQKLIVSSALAGLIAMASAGNTFAEDKPASKEKCYGVAKAGKN
                     DCANATGTHSCAGQATTDKAPEEWNYVAKGTCEKLGGNLAFKK"
     gene            8841..9686
                     /locus_tag="VN496_01815"
     CDS             8841..9686
                     /locus_tag="VN496_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXF15705.1"
                     /translation="MSLLGVPDAKPVAHGVGIGLRAVHYRPFLDSRPAVGWLEVHSEN
                     YFGEGGYDLHVLEQLRHRYPISLHGVALSVGSADGIRKAHLAKLKRLVEHIEPALVSE
                     HLCWAALGRRHFNDLLPLPYTAEVLDLIVAQVSQVQEALRRPILIENVSSYLQFRASE
                     MPELGFLAELAKRSGCGILLDLNNLFVNSVNHDFDPVEALDAIAPGTVCEIHLAGHSA
                     AGEVLIDDHGCRVSPPVWNLYRIACRRLGALPTLIEWDTAIPPLSVLLDEAATAERLR
                     EAEYV"
     gene            9679..10461
                     /locus_tag="VN496_01820"
     CDS             9679..10461
                     /locus_tag="VN496_01820"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXF15706.1"
                     /translation="MSDLAAMQALFGWGLDATDAQADALTMFLGGAEQIKQRFAIYRA
                     NTLANAVKALEAAYPVVAKIVGSEFFSGLAREYRLRFASQAGDLNEYGEFFADFIDGF
                     APARDMAYLADVARLEWLVHRAHYAADPAPFDPAGLAAVPSQQQLELRPRPHPACHVL
                     RSVYPIARIWQVHQDAFSGVFEVDFSQKPGFALVSRPHFKVEVTCISDAEAAFLSAAL
                     SGATLASALTAAQERDDAFDLGRSLSAWVQSSVIVDFGLDGA"
     gene            10463..10972
                     /locus_tag="VN496_01825"
     CDS             10463..10972
                     /locus_tag="VN496_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXF15707.1"
                     /translation="MLRIDKLLPVLNICRNALNRLADVLDLVIRFYIAKVFLLSGLSK
                     LRDWDTTLTLFQEEYHVPVLSPELAAYLGTSGELVLPVLLVLGLATRFAAFGLTFVNL
                     MAVVSYWHVLSMAEPALAQHVYWGVLILVVLLHGPGRISFDAMLWKKINPADKASSRS
                     SNSVGLFRL"
     gene            11095..11865
                     /locus_tag="VN496_01830"
     CDS             11095..11865
                     /locus_tag="VN496_01830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15708.1"
                     /translation="MTVNHCASYPSLKGKVVFISGGGSGIGASIVEGFVRQGAGVGFI
                     DIDEAASNVLVRTLKGVGPTPRFVHCDVRDIEQLRMAIGMVQSELGDISILVNNAARD
                     DRHSVESVTVEYWDERLAVNLRHQFFAAQTVVDQMKRLGGGSIINFGSISWMTATGGM
                     PAYTTSKAAVHGLTRGLARDFGPFNIRANTVSPGWVMTERQKQLWLTPEGERQLDASQ
                     CLKGRLQPADIANMVLFLASDDAKMITSQNFVVDAGWT"
     gene            complement(11938..12843)
                     /locus_tag="VN496_01835"
     CDS             complement(11938..12843)
                     /locus_tag="VN496_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HXF15709.1"
                     /translation="MKSKPFISISAGTLHLDIAPEIGGSIARFYSKEKGDIMEWMRPA
                     SAEGLAAGDPQMMSSFPLVPFSGRVRDGRFVFAGRHITLPRNFGNSPHAIHGNAWKLP
                     WKVTAHDPASISLVLDHERGDWPFAYRAEQHFVLTPRSLEIELIVRNTDAATMPVGIG
                     QHPYFAYTPGMTLHAEVTQMWEAEHDMPVKLTRNDVVEALARGLKMSTVTLDNNFIGW
                     KHMATLRWPETGRRLRMTAQPPLSYLVVYTPPSREFLCVEPVSNTVDWLNLGHLESEQ
                     IGGTILSPGASLSGLVRYEPDFGTG"
     gene            complement(12840..13691)
                     /locus_tag="VN496_01840"
     CDS             complement(12840..13691)
                     /locus_tag="VN496_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF15710.1"
                     /translation="MIDAHHHLWQLARADYGWIGHGSDPALAPIERDYLVEDYRRVAE
                     ANGVSGSIAVQAAQTVAETRWLLQQARHSGELIRGVVGWIDMAAADATEILCELAKDP
                     LLRGIRPMLQDIPDTEWVLQPGIGPALRAVVELDLSFDLLVRPPHLKAALVLLGRHPD
                     LRAIIDHGGKPQIAQGMWQSWADDMRRLAHETRVYCKLSGLITEARAGCKPDDLRRYA
                     EHLIECFGADRLVWGSDWPVMLLNGDYDGWLAATQDFISGQSTADRAAIMGDNAIRFY
                     KIAVRKP"
     gene            complement(13688..14695)
                     /locus_tag="VN496_01845"
     CDS             complement(13688..14695)
                     /locus_tag="VN496_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF15711.1"
                     /translation="MLNRKPLGRSKLDLPVLGFGGTGLGNMYVAMSERSAIDTLSAAY
                     AAGISYFDTAPLYGHGLSERRLGCGLRLFADPGVVVSTKVGWRLRPAFGKPTGADLFR
                     DITDFARFNDYSYDGTMRSFEDSLNRLGTDRIDILLIHDVDHRNQGDRYPEVFDQAMA
                     GAYKALLSLREQGVVKAIGCGLNEWEACQAFAEAGDFDCFLLAGRYTLLEQESLQSFL
                     PLCGRRGIGIILGGPYNSGILASGAVQNAWFNYAPATAEVLARVHAIEKMCAAHGVPL
                     KAAALQFPLHHPCVASVVPGVSSVPELNENLRMLNAPIPERFWRDLKSAGLMDAQAPT
                     S"
     gene            14852..15286
                     /locus_tag="VN496_01850"
     CDS             14852..15286
                     /locus_tag="VN496_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HXF15712.1"
                     /translation="MLKNIDPLLSPELLSTLRAMGHGDELAIVDANFPAMSSGRPVLR
                     ADGALVPAVASAVLTLMPLDEFVPCAAFHMQVVENPAGQMPIFEEFRSLLRKYEPKMA
                     KLEGIERFAFYERVKRCFAVVATGERRLYGNLILKKGIVRPD"
     gene            15299..>16013
                     /locus_tag="VN496_01855"
     CDS             15299..>16013
                     /locus_tag="VN496_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF15713.1"
                     /translation="MRLFELRGISKNFGAIQALAGVNLKIDAGEVLGLMGDNGAGKST
                     LIKIMAGNFPPSAGEIWLKGAKVEFNNPVDARRKGIEVVYQDLALCDNLTAAENVFLG
                     REAKFKFGPFSLVHYSAMFREAARHFAELKSETRPRDLVKQMSGGQRQAVAIARTRLS
                     NPDIVLMDEPTAAISVRQVAEVLDLIRRLRDQNVAVILISHRMPDVFAVCDHVSVMRR
                     GRIVADKPIRQTSPEEVTGL"
BASE COUNT         3469 a         4434 c         4623 g         3487 t
ORIGIN      
        1 cgaagatgcc aagttctgcg ccattgacaa tccagaatgc gaggcttgcc agtgagcggg
       61 aagccggcgt tattgattgc ggcgcaggct aacttggcgc ttgcccagtt aagcgcagtg
      121 cgcggccgta gccacaatcc agaatgcgag gcttgccagt gaagggtaaa gccggcgtta
      181 ttgattgcgg cggaggctaa cttggacttt gccaagttaa gcgcgttgcg cggccgcaac
      241 cacaatccag aatgcgaggc ttgccagtga agggtaagcg ggcgttattg attgcggcgc
      301 aggctaactt ggactttgcc aagttaagcg cgttgcacaa caggaacgac aaccccggca
      361 tgcgaagcat gccagtggct gagcaccatg gcctttatgt ttcctgcatt ttggtggatt
      421 gcgcgcgtca tgacattcgg ttgtcttcgc catgtcggct gttggctgaa ccgagaatgc
      481 agtcccagac gcgaagtctg ccaataatgg tcagaaagaa acggcaagtt gccaataatg
      541 gtcagtacag aacgttgttg cgacaggaag cgttgccgtg aaactctctc gaccatttaa
      601 cacgaccgct cggcgtcgac tgacattctg gttgcgccgt gcgcgcgaag aaacgatgcg
      661 acccgtctcc gcggcaaagt cctggaagag gagttaaatc gttttttgag ttgatgaacc
      721 gatcgatgcg attttcgtcg tgacaaatgc cgaaaaagtg gtcgcatcgc ggctgaaata
      781 tcaagaagta tttgacattc cacccataac atttataaag gaacaattga tgttgcaatt
      841 tgaagaacat attccgaaca ccggcggtgc aggtctgcag accgctgcga atgttccgcc
      901 agcgcgcctt tccggagccg gccacgccat gtcccaggat gtgacgctgc gccgtgttcg
      961 ggtagaagac aagcgcatca ttaacggcaa agccgacgtc aatcagctgg tcccgttcaa
     1021 atacaaatgg gcctgggaaa aatatctttc cggatgtgcc aaccactgga tgccccagga
     1081 aatcaatatg agccgggaca tcgctacgtg gaaagaccca aatggcctca cggaagacga
     1141 gcgtctggtc gtcaagcgca atctgggatt cttcgttaca gccgacagcc ttgctgcgaa
     1201 caatatcgta ctgggcacct accggcacat tactgctccg gaatgccggc aatatctgtt
     1261 gcgtcaggcg ttcgaagagg caattcatac ccacgcctac caatacatcg tcgaaagcct
     1321 gggcttggac gaaggcgaga tattcaatgc ctatcacgag gtttccagca tcagggagaa
     1381 agatgaattc ctgatcccgt tcatcgaaac gctgaccaac ccggaattca ggaccgggac
     1441 cccggaaacc gaccagcagc tgttaaagag cctcattgtc tttgcttgca tcatggaggg
     1501 gctgtttttc tatgtcgggt tcgcacagat tcttgccctc ggccgccaga acaaaatgac
     1561 cggcgccgcc gaacagtacc agtacattct gcgagacgag tcgatgcact gtaatttcgg
     1621 cattgatctg atcaatcaga tcaagatgga gaatccgcac ctttggacgc cgcggttccg
     1681 tgatgaaatc cgcgacctga tgcagcaagg cgtggaactg gaataccgct acgccgagga
     1741 caccatgcca cgtggggtac tgggactgaa tgcaccgatg ttcaaggaat acctgcgcta
     1801 cgtggccaat cgccgtgcgc aacagatcgg cctggatccg ctttatcccg gagcgaataa
     1861 tccgttccca tggatgtccg agatggtgga tctgaagaag gaaaagaatt tttttgagac
     1921 ccgtgtgatc gagtatcaaa cgggtggagc cctgaattgg gattgacctt cagggccgcg
     1981 aagctttctg tgagacccgc gttgccatgt tttgcatcag gggcagcgca tttgacgggc
     2041 tgaatgggta aaccggacaa agctgtatct ctacattacg aattgtgtgc cggcccttac
     2101 agggccggtg cggcaggctg ggagccatga tttcccggtc caaggttgtc ttttcttttc
     2161 gttttaggtc cctgacccgc gatggctatc catcgttttg ggatctgttc ccacatacct
     2221 ccccaaatta cgctgcgcgc ggacgtcccg cgttcggcca acagaactgt cggatgtccg
     2281 gacatgcggc ctgcatagcg caattccgtt catcggtagt cggcacggag aagaaatcct
     2341 tctttgacgt gctggtagtg ccctttgttt aactttcatt aggagatata gcaatggcag
     2401 caaagaaaaa agcaacgaag aagaccgcga agaaatcgtc agccaagaaa aaacccgcca
     2461 aaaaggctgc gaagaagtct gcgaaaaagc ctgcgcggaa ggtagcgaag aaggctgcta
     2521 agaggcctgc gaaaaaggct gctaagcgcc cggccaagaa aaaggccgca aagaaaaagg
     2581 cgaagaaacc cgccaaaaag aaggcgaaga aggcggcgaa gaagcctgca aaaaagaaag
     2641 cgaagaaggc ctccaagaag aagcctgctg ccaaggcgcc tgctgctgca gccccagcgg
     2701 caccgaaacc ggtgtccgct cctgcggctt ggccctttcc ggtagcgggc aacaggccta
     2761 actaagcttc ccatatgctc gcgggcaaac tgcatccgcg agggatacca ggcgctgagc
     2821 cgcgagcttt gggattcgca gtacgaacaa agctgcactt gtgaaagtgc ggtttcgtta
     2881 ggattgatgg ataacgctcg ccgatcgaag ggcgaagctg gtttaatgtc ctaacagtag
     2941 ttctcgccct tggtgggacg agagggattt tccattccaa tgggacttca aaggttaagc
     3001 gcttcaaata ttacggattg ttccgccttg gcttccgaac ggcatcccga tccgacgggg
     3061 aaagcattct tgctgtagat ccggttgaat cgccattcca gcatggcgtt gcctgcgttg
     3121 tcagtcggac gcggcatcta atgcggtgtc atctcagttc ataaacagga gaaaatttga
     3181 aagcgccaag cggaaaaatc ggtttgttat gccttgtcgt tttcatcatt gcgatgattg
     3241 gcgcattcgt tcccatgggt cacgtgccct atattgggtc cgggctaact ctggtcaacc
     3301 actatcatga gtggttgctc atgtccggat acgcgttgct gctgctcgca atctacattc
     3361 tgtaaaagac ggggccggat gcaagcccgg cccatgcgtc ttctgcaccc gttaccgatt
     3421 cggttgaggg gtgatccgaa ggtatggttt aaccgcttta tagcctttcg gaaatttttg
     3481 cttgatgact tccgggtcct gtatcgatgg cacgatcact acatcatcgc catccttcca
     3541 gtttactggt gtcgctacac tgtagcggtc cgtcagctgc agagaatcga tcacgcgaag
     3601 aatctcatcg aaattgcgac cggtactggc aggataggtt agcgtcgtgc gtattttctt
     3661 gtttggatcg atgatgaaaa cggaacgcac ggtcaaagtg tcgtttgcat tgggatggat
     3721 catgtcatac agattcgaca ctttcttgtc tgcatccgcc aggatcggga aattcaattt
     3781 tgtattctgt gtttcctcga tatccccgat ccatttattg tgggaatcga gcggatcgac
     3841 actgatggca atggctttga caccacgctt gtcgaattcg gacttcagtt tggcggtgta
     3901 gccaagttcc gtagtgcaga cgggggtaaa gtccttggga tgtgagaaca gcactaccca
     3961 attattgccg gcccattggt gaaatttgat ggttccttgg gtggattcct gtgtaaagtc
     4021 tggggcgata tcgcctaggc gaagagtggc cacgattgtt ctccaatagt ttgtcgatgg
     4081 ggtcgaatac tttactcgtc gcattcttat ttcacaaact accggtttta cataatgtta
     4141 tgccttgaat ctacataggg cagacgtcat ggatagaaag aaagtcaatt ccagttccat
     4201 tcggtccgtc ggctacgatg agcgcaatcg catgctggaa gtggagttca acgacggtcg
     4261 gatcatgcaa tattccggcg tttcggcgga gcttcaccgg cgcttgatga atgcgccttc
     4321 gatggtgagt tacttcaggg acaatatcga agagtcgttc gcagcgaaac gcgtgaaagg
     4381 ttgaggcaag tcgccttact accgtaacgc ttaacactcc ggctctccat ttgcaggttg
     4441 cggcgcggga tcgaagccaa atttctgagc ggcttcgccg gcctggatcc ggcctatcgg
     4501 cacacggata cccgcgtatt caatgtaaat accggtcgcc tggcgacgaa attctcggtc
     4561 gaacacgcga cccagcaagc catcgtcgac tgcattgccg tgcaggtctg tgaacagtcc
     4621 cagaattcct ggtaagtcgc ggtcgtccag aaccccgact tcgctgcccc agcgcagcag
     4681 cagcgaaccc atctcatcga tccaggcgcc cgacagcttt ccgatcgggc agttaatatg
     4741 ggtttcgagc gatggagggc cgtcgcccga agtgcggacc acaaacggtg tgtagtctag
     4801 agcaacgaaa acacgctgtg gcccgttttg aaaaaaccag cgtccccgct catcgacacc
     4861 ataattgcga ccgatgaagg ctgtgacgcc ggaattggtg atgtgctcac ccttgattag
     4921 ccaatcaccg cgtcggttta gccgcaacca gccgtatacg tgggggacgt ccggccattt
     4981 gcccatcgcg cgaatgacga tatcgtccaa ttatttcccc tgaaccgcca ctgcgaagcc
     5041 ggacgaccag tggccgctcc cgcgcgagcg tgactcgtta gcggaacgcc cgtctgcggg
     5101 aacgcgagcg actcggtatt gggacgcatc cacgctcgct gatgactcga ctccgcccca
     5161 agctgcgccc gccggagagc cgagtccggg cgtaccgcta acgagtcgcg gccacacatc
     5221 cagcaatttg cccatcgcgc gaatgacaat gtcgtccaat tatttcccca tatctgcgac
     5281 cctgaacccg gacgtccgct ggccgccccg cgaatggcag ttccgataag catccggctc
     5341 gtaatgagca gcaatcggaa taacagcacg accacggtaa aattgtactc atgccccaac
     5401 agcggcgtac agccttcacc aattaataac tcatcgaagg acgtcaatgg ctctgactct
     5461 ctattacggc tccggatcac cctacgcttg gcgcgtctgg cttgcgctcg aacacaaggc
     5521 gattccctat caactgaaga cggtatcttt ctcagccggc gatttgacca aacctgaatt
     5581 ttccgcgttg aatccacgac acaaagtgcc aaccattgtg gatgatgatt tcgtcctcta
     5641 cgagtctgcg gcgatccttg aatatctgga ggaaaaatat caatccggga aaacgctatt
     5701 tcccggaaac gtaaggcagc gggctgtcat tcgtcgcctg gtgcgtgaag cggaccagta
     5761 tctgacgcat agcatggaac gcgtgctgga tcaggtgctg ttcaccgagc aggatcaatg
     5821 ggacctgaaa gagatagcgg ttggacgcgc cgattttttg acagaactgg acctgttcga
     5881 aagctatctg cgcggagact ggttcggcga cggattgtcc gccgcagacc ttacaatcta
     5941 tccgatgctc gcgctgtgcc tgcgcatgga aaagaagaag ccggacctcg gcctgaccac
     6001 atccattccc ccgaagatca gtttgtggat gaagcgcatg caagcacttc cttattacga
     6061 caagacgtat cctccgcact ggaaggcaaa cccctgagcc agtatttctg gcgttgtaga
     6121 ttgacctctt tcgatgcctg aataaccagt agacatggct cagtgcccga aagatgcttt
     6181 tgctgccagc agtgatttca gctccacaag ttgaatgtcc cgagctgttt cgattttctg
     6241 ccacgtcatc cgctctggta tcagatgcgg gcgactacgg gcttaggtca ccgcgtttgg
     6301 taacatagcc tgcaaataag caatcagaaa gggtagcttc gtgagcgcag tcaaccaacc
     6361 gcgaatggca ccatgagcat cttgttaggg tgcgttgccg atgatttcac cggagcgacg
     6421 gatcttgcca acaccttggt caaggctggc atgcgcaccg tgcaactaat gggcgtgcct
     6481 cgcaggacgt tgaccctgcc ggcggccgat tgcatcatcg tcgcgctgaa gtcgcgcagc
     6541 aatccggcct ccgaggccgt cgcaatgtcg cttgccgcac tggactggct gcgcgaaaga
     6601 ggcgcgcaac agttctactt caagtactgc tcgacgttcg actcaactga caaggggaat
     6661 atcgggccgg tggcggacgc gatgctagac gcgcttggcg ctgacttcac catattcaat
     6721 cctgcatttc cgaccaacaa gcgcacggta tataaggggt acctttttgt cggagatgaa
     6781 ttactctccg agtccggcat gcgccaccat ccgcttaccc cgatgactga cccatcgctg
     6841 gtgcgcgtgc tgcagcggca gactgctcat aaggttggat tggtgcaatt ttcaacgatt
     6901 gcgaaggggg tttccgcaac gcgcagcgcg ttggaggatc ttcgcaagca gggtgcccgc
     6961 catgctgtac tggattccct gactgacgaa catctcatga cgctaggcga atcctgtgcg
     7021 gatatgaaac tcgttactgg tggttccggc atggctatgg gcctgccggc aaattttatc
     7081 cggcagggcc tattgaaagg cggtcagcgc gccaagctcc ctaaagtgac tggtccgacg
     7141 gccgtaatcg cgggctcgtg ttccatggca acgcaaggcc aggtcgcatt gatgagcaag
     7201 gatcatgacg cattcgaact cgacccgatg gctatcgcct ccggtcgcga cgttacgcgc
     7261 gatgccctgg actgggcggc gcagaggctt tcggaaaaac cgattctgat ctactccact
     7321 ggaacccccg agaaggtcaa agcggtgcag gagcggctcg gccgcgagca cgcaggcgcc
     7381 gtggtggagg agaccttcgg caaaatcgca aagggcctgg tcgcgaaggg cgtgcgcagg
     7441 ttgttggttg ctggcggcga aacttccggc gcggttgtaa gcgcgctggg cgtggaagga
     7501 cttgctatcg ggcccgaaat cgatcccggc gtgccctgga cgttttcgat cggtgaaccg
     7561 tcgctggcgc tggcattgaa gtcaggcaac tttggcaccc ccgatttctt taccaaggcg
     7621 ttcaaggttc aacccatgta gttcaagcgg cttttcgctt gtccaggtgc gccgttgcgt
     7681 gcggttaact cggtctcact atctgcaaaa tgaatgaaac caaactaagg gaagagatct
     7741 gcatgctcgg caagtccatg ttcgaccgcg ggctcacggt cggcagctcg ggcaacatca
     7801 gcgtgcgcct gaacgatggc tggttgatga cgccgaccaa ttcctgcctg ggtcgcctgg
     7861 acccggaggc tctttcaaag gtcgacgcca atggcaggct gctttccgga ggcaagcaga
     7921 ccaaggagtc ctttctgcat cttgcagttt acggcgagcg gccttcagct ggcgccatcg
     7981 tgcatctgca ttccacgcat tcagtggctg tttcctgtct cgcggagatt gatccacagc
     8041 agccgattcc gccaatcact gcctattacg tcatgaagat cggcaacctg atgctgatac
     8101 cgtattacgc accgggagac atggcgcttg ccaatgcggt gaaggaagtg gcgggaaagc
     8161 atcacgccat tttgctggcg aatcacggtc cgatcgttgc tggcaaggat ctggagagtg
     8221 cggtttacgc aaccgaggaa ttggaggaaa ccgctaagct ttatctgttg ttgcgtggtg
     8281 accggctcaa catcctcacg caagaccagg tagcggaatt gaaccggaag tggccgcagt
     8341 aaaatgcagc caggcacaag ctgaaatttc ctttagctat gcttgcccgc atccggccgc
     8401 tgattctcaa cgtatctgtc cgtgtgcgcg gacaccaaac gggagctccg cccgcaacgc
     8461 cgtaccgatt catatagcca acctttcaat ctcgttccaa ccaaggagat ttgcatgaac
     8521 cagaagctta tcgtcagttc cgccctcgcc gggcttatcg caatggcttc ggcaggcaac
     8581 acctttgccg aggacaagcc ggccagcaag gaaaaatgct atggcgtcgc caaagcgggc
     8641 aagaacgatt gcgccaatgc cacgggaacg cattcctgtg cgggacaggc cacgaccgac
     8701 aaggcgccag aagaatggaa ctatgttgcc aagggtacgt gcgaaaagct cggcggcaat
     8761 ctggcgttca agaaataata tcgtagtgct gtccgtcggc acggagcaga tggttcgctg
     8821 gtgccgatgg ccttcacatt atgagtctgc ttggcgttcc ggacgcaaaa ccggttgcgc
     8881 acggtgttgg aatcgggttg cgcgcggtcc actaccggcc ttttctcgat tcccgaccgg
     8941 cggttggctg gctggaggtt cacagcgaga attatttcgg tgaaggcggc tacgacctgc
     9001 atgtgctgga acagttgcgc caccggtatc cgatcagtct gcacggcgta gcgttgtccg
     9061 tgggctccgc cgatgggatt cgaaaggcgc atcttgccaa actgaaacgg ctggtcgagc
     9121 acatcgagcc ggcgctggtg tcggaacatt tgtgctgggc cgcgcttggc cgccgtcact
     9181 tcaatgatct tctgccgctg ccttataccg ccgaggtgct cgacctcatc gttgcacagg
     9241 tgtcgcaagt tcaggaagcc ttgcgccggc cgatactgat cgagaacgta tccagttacc
     9301 tgcaatttcg cgcctccgag atgccggaat taggctttct ggcggaactc gccaaacgca
     9361 gcggctgcgg tatcttgctc gatctcaaca atctgttcgt caattccgtg aaccacgatt
     9421 tcgatccggt tgaagcgttg gatgcaattg caccgggaac tgtttgcgaa atccatctgg
     9481 ccggtcactc cgccgccgga gaagtgctga tagacgatca tggttgccgc gtctccccgc
     9541 cggtctggaa cctttaccgg attgcctgcc gtcggctggg tgccctaccg acgctgatcg
     9601 aatgggacac agccattccg ccgctcagcg tgttgcttga cgaagccgcg acggcagagc
     9661 gcctgcgcga ggctgaatat gtctgacctt gccgcaatgc aggcgctgtt tggatggggt
     9721 ctggacgcta ctgatgcaca agccgatgcc ctcaccatgt ttctcggcgg ggccgagcaa
     9781 atcaaacagc gctttgcgat ttatcgcgcc aacacgctgg ccaatgcagt caaggcgctc
     9841 gaagcggctt atccagtggt cgcaaaaatc gtcggcagtg aattcttctc tggattggcg
     9901 agggaatacc gtttgcgctt tgcgtcgcag gcaggggact tgaatgaata cggtgaattc
     9961 ttcgctgatt tcatcgacgg ctttgcgcct gcgcgggaca tggcttatct ggcggacgtg
    10021 gctcgcctgg agtggttggt gcatcgagcg cattacgccg ccgatccggc acctttcgat
    10081 ccggcaggtc tggccgcggt gccttcgcag caacaactgg agctgcggcc acggccgcat
    10141 ccggcttgcc atgtgctgcg ttctgtctat cccattgcgc gcatctggca agtgcaccag
    10201 gacgcattca gtggtgtctt cgaagtcgat ttttcacaga agcccggttt cgcgctcgtg
    10261 tcacgccccc acttcaaagt cgaggtcacc tgcatcagcg atgctgaagc tgcgtttctg
    10321 tccgcagccc tgagcggtgc cacattggca agtgcattga ctgccgctca ggagcgcgat
    10381 gacgcgttcg accttggtcg ctcgttgtcc gcctgggtac agtcgtccgt gatcgtcgat
    10441 ttcggactcg acggagcata acttgttacg catcgacaaa ttactgcctg tcctgaacat
    10501 ttgccggaat gccctgaacc ggctggctga cgttcttgat ctggttatcc ggttttacat
    10561 cgcgaaggtt tttttgctgt ccggcttgag caaactgcgc gactgggaca cgacgctgac
    10621 gttgttccag gaggaatacc atgtgccggt gctttcgccg gagcttgccg cgtacctggg
    10681 cacaagcggc gaacttgtgc tgccggtgct gttggtgctt ggtctggcaa cccgcttcgc
    10741 ggcgtttgga ttaactttcg tcaatctgat ggcggtagtc tcgtattggc atgtcctgag
    10801 catggccgaa cccgctttgg cacaacatgt ttattgggga gtattgatcc tggtggtact
    10861 gttgcacggg ccaggcagaa tttcgttcga tgcgatgtta tggaaaaaaa tcaatccggc
    10921 cgacaaggcg tcgtcgcgtt cgagcaattc agtcggattg tttcgtcttt gattattgcc
    10981 acactggcca tgggctgtgt ggcgactgca taatgccgtg gaatccctgt tgttcaggga
    11041 agcggaaaaa cccaaaaata gaaaacgcgc tcagctgaac tcacagggat tgccatgact
    11101 gtcaatcatt gcgctagtta tccgagcctc aagggcaaag tcgtattcat tagcggcggc
    11161 ggttccggaa taggagcctc catcgtcgaa ggcttcgtgc gtcagggcgc cggggtgggc
    11221 ttcatcgata tcgatgaagc ggccagtaat gtactggttc gcacgctcaa aggtgtcgga
    11281 ccaacgccac gcttcgtgca ctgtgacgtt cgcgacattg agcaattgcg catggccatc
    11341 ggcatggtgc aatcggagct cggtgacatc tcgattctgg tgaacaatgc agcgcgtgat
    11401 gaccgccact cggtcgaaag cgtaacggtc gaatactggg acgagcggct ggccgtcaat
    11461 ctgcgccatc agttttttgc ggcgcagacg gtcgtggacc agatgaaacg cctcggcggg
    11521 ggatccatta tcaactttgg gtccatctca tggatgaccg caaccggtgg catgcctgcc
    11581 tacaccacca gcaaggcggc cgttcacgga ctcacacgag ggctggcgcg tgacttcggt
    11641 cctttcaaca ttcgcgccaa taccgtttcc ccaggctggg taatgaccga gcggcagaaa
    11701 caactctggc ttacgccaga aggtgagcgc cagctggacg cgtcgcaatg cctcaagggc
    11761 cggctgcaac ccgcagacat cgccaatatg gtgctgtttc tggcatcgga tgacgcgaag
    11821 atgatcacct cgcaaaattt cgtagtggat gcgggctgga cgtagaagcc agtaaagtaa
    11881 tccctcggat aacaatttac tgcgttaaag ctacgcgcgc gccgttcaaa tactgcgtca
    11941 acctgtgccg aaatcgggtt cgtagcgcac gagtccggac agcgatgcac cgggcgaaag
    12001 aatagtgccg ccaatctgct cggactcgag gtgacccaga ttgagccagt cgaccgtgtt
    12061 gctcacaggc tcgacgcaca ggaactcccg agacggcggg gtgtagacga caagataaga
    12121 cagcggcggt tgtgccgtca tgcgcaagcg gcgtcccgtc tctggccagc gcagggtggc
    12181 catatgcttc cagccgataa aattgttgtc cagcgtaacg gtgctcattt tcaatccgcg
    12241 tgccagcgct tcgaccacat cgttacgtgt caatttgacc ggcatgtcat gctcggcctc
    12301 ccacatttgc gttacttctg cgtgcaatgt catgccgggc gtatacgcga agtaaggatg
    12361 ctggccgatg ccaaccggca tcgtggcggc gtcggtattg cgtacgatga gttctatctc
    12421 gaggctgcgc ggtgtcagca cgaaatgctg ttccgcccga tacgcaaacg gccagtctcc
    12481 gcgttcgtga tccagcacca ggctgatgga agcagggtca tgtgcggtga ctttccatgg
    12541 cagcttccag gcgttgccgt gaatggcatg cggcgagtta ccgaagttgc gcggcagtgt
    12601 tatatgccgc ccggcaaata caaaacggcc gtcgcgcacg cgaccgctga atggcaccag
    12661 gggaaaactc gacatcattt gcgggtcgcc ggctgccagc ccttccgcgc tggccggccg
    12721 catccattcc atgatgtctc ctttttcctt gctgtagaag cgcgcgatcg agccgccgat
    12781 ttccggggca atatccaggt gaagagttcc agcggagatg ctgatgaagg gtttgctttt
    12841 catggcttcc tcaccgcaat cttatagaag cgaatcgcgt tgtcgcccat gatcgcggcc
    12901 cgatccgctg tggattgacc ggagataaag tcctgtgtgg cggcaagcca gccatcatag
    12961 tccccattga gcagcattac cggccaatcg ctgccccata ccaggcggtc cgccccgaag
    13021 cactcgatta gatgctcggc gtaccggcgc agatcatcgg gtttgcaacc agcgcgcgct
    13081 tcggttatca gcccggacag cttgcagtac acccgtgtct cgtgtgccag acggcgcatg
    13141 tcatcggccc aggattgcca catgccctgc gcgatctgcg gcttgccccc atgatctatg
    13201 attgcgcgca gatcgggatg tcgtccaagc aacaccaggg cggccttgag atggggcggc
    13261 cgaaccaata agtcgaatga cagatcgagt tcgacgacgg cgcgcaacgc cggaccgatt
    13321 cccggctgca ggacccattc ggtgtcgggt atgtcttgca gcatgggtcg aattccgcgc
    13381 aggagcggat ctttcgccag ttcgcacaaa atctccgttg cgtccgccgc cgccatgtcg
    13441 atccagccga ccactcctct gatcaattcg ccgctatgcc gtgcctgctg caacagccag
    13501 cgggtttcgg ccacggtttg cgcggcttgc acggcgatgc tgccggacac gccgtttgct
    13561 tcagccacgc ggcgatagtc ttcaacgaga tagtcgcgct caattggcgc caatgccgga
    13621 tcgctgccgt ggccaatcca accgtagtca gcgcgcgcca gctgccacag gtgatgatgg
    13681 gcgtcgatca tgaagtcggc gcttgtgcat ccatcaaacc ggccgacttg aggtcgcgcc
    13741 agaatcgttc gggaatcggc gcattgagca tgcgcaaatt ctcattgagt tccggcacac
    13801 tgctcacacc cggtaccacg cttgcaacgc agggatggtg cagcggaaac tgcaacgcgg
    13861 cagctttgag cggcacgcca tgcgccgcgc acattttttc gattgcatgg acccgtgcaa
    13921 ggacttctgc tgttgccggc gcgtagttga accaggcatt ctgcacggcg ccgctcgcaa
    13981 gaatcccgga attatatggg ccgccaagga taatgccgat acctcgcctg ccgcagagag
    14041 gaaggaaaga ttgcagcgat tcctgttcga gcagcgtgta gcggccagcc agcaggaagc
    14101 agtcgaaatc gccggcttcc gcaaaggcct ggcaggcttc ccattcgttt agcccgcagc
    14161 cgatcgcctt cacaacgcct tgctcgcgta gcgaaagcag cgccttgtaa gcgcccgcca
    14221 tcgcctggtc gaatacctcg gggtagcgat cgccttgatt gcgatgatcg acgtcatgga
    14281 tgagcaggat gtcgatgcga tctgttccga ggcggttgag actatcctcg aacgaacgca
    14341 tggtgccgtc atagctgtaa tcgttgaagc gcgcaaagtc cgtgatgtcg cggaacaagt
    14401 cagcgccggt cggtttgccg aaagccggac gcagccgcca tcccactttg gtggaaacga
    14461 cgacgccggg atcggcgaac agtcgcagac cgcagcccag ccgcctttct gaaagtccat
    14521 gtccgtacag cggagcagtg tcgaaataac tgatcccggc tgcgtaggcc gcagagaggg
    14581 tgtcaattgc gctgcgctcg gacatggcga catacatatt gccaagcccg gtgccgccga
    14641 aacccaacac cggcaaatcc agcttcgaac ggcccagggg ttttcgattc agcatctctc
    14701 ctccgcgaat gcgggccgat ggtagcacgc tgatatgccc ggaccgggct ggtttttttg
    14761 cggcgcaacc agaaatgcgc ttgctctccg gcgcgaatct gcgggatgct tgccggaata
    14821 ttgggacaca gaatctgttg aggttgctgc agtgctgaag aacattgacc cgctgttgtc
    14881 gcccgaattg ctttcaacgt tgcgggcgat gggacatggt gacgagctcg cgattgttga
    14941 tgccaacttt cccgcgatgt cgagtggccg tccggtgctc cgggccgacg gtgcgctggt
    15001 gccggcggtc gcgtcagcgg tgcttacatt gatgccgctt gacgagttcg tgccttgcgc
    15061 ggctttccac atgcaggtcg tggaaaatcc ggcaggccag atgccgatct tcgaggagtt
    15121 ccgatcactg ctgcgcaagt acgaaccgaa aatggcaaag ctcgagggca tcgaacgctt
    15181 cgcgttctac gagcgggtaa agagatgttt cgccgtggtc gccacgggag aacgccggct
    15241 ttacggcaat ctgatcctta aaaaaggcat cgtccggcct gactgaagag gaagcaacgt
    15301 gcgtttgttc gaactgcgcg ggatttcgaa gaatttcggc gccatccagg cactggccgg
    15361 cgtcaatctg aagatcgacg ccggtgaagt gctcggtttg atgggggaca atggcgccgg
    15421 gaaatccacg ctgatcaaga tcatggcggg caattttccg ccatccgccg gtgagatctg
    15481 gcttaaaggc gcgaaagtcg aatttaacaa cccggtggat gcacgccgca agggcatcga
    15541 agtcgtttat caggacctgg ccttgtgcga taacttgact gcagcggaaa acgttttcct
    15601 tgggcgcgaa gccaaattca aattcggccc gttcagcctg gtccactatt cggcaatgtt
    15661 ccgcgaggcc gcgcgccatt tcgcagaact caaatcggaa acgcgcccgc gcgatctggt
    15721 aaagcagatg tccggcggtc agcgccaggc tgttgccatc gcgcgcacgc ggctgtcgaa
    15781 tccggatatc gtgctgatgg acgaaccaac tgcggccatc agcgtgcgcc aggtcgcaga
    15841 agtactcgac ctgatacgcc ggctgcgcga tcagaatgtc gcggtgatcc tgatcagcca
    15901 tcgcatgccg gacgtatttg cggtgtgcga ccacgtctcg gtcatgcgcc ggggaagaat
    15961 agtcgcagac aagcccatcc gccagacttc ccccgaagaa gtcaccggcc tga
//
LOCUS       DATMZQ010000052         5208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2455501, whole genome shotgun sequence.
ACCESSION   DATMZQ010000052 DATMZQ010000000
VERSION     DATMZQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455501"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..762
                     /gene="nth"
                     /locus_tag="VN496_01860"
     CDS             106..762
                     /gene="nth"
                     /locus_tag="VN496_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXF15714.1"
                     /translation="MNPAKRAEIFRRLRAANPAPTTELVHGTPFELLIAVILSAQATD
                     KSVNLATAKLYAVADTPQKMLALGEDGITPYIQRIGLYRTKAKNVIATCALLVEKFGG
                     EVPSNRVDLESLPGVGRKTANVILNTAFGQPTIAVDTHIFRVANRTGIAPGKNVTEVE
                     NRLLKFVPGEFLQNVHHWLILHGRYVCKAQRPDCPACLIRDLCEFKAKTKADVQSVAR
                     "
     gene            740..1168
                     /locus_tag="VN496_01865"
     CDS             740..1168
                     /locus_tag="VN496_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HXF15715.1"
                     /translation="MFNPSRADVRRFFFETWRKYRLHLPLEGLERTAIEIILMHPEHY
                     PLLDNPERNLETEFTPEDGAVNPFLHLSLHLAIAEQLSIDQPRGIAVAYRELAGRTAS
                     PHDALHALLECLGEIVWLAQRGGTAPDELAYLDCIRRTLR"
     gene            complement(1254..1586)
                     /locus_tag="VN496_01870"
     CDS             complement(1254..1586)
                     /locus_tag="VN496_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF15716.1"
                     /translation="MKNMLIAIIALVACGATGHAMAGGDATKGKEKAGLCSTCHGPEG
                     NAPIAPEYPRLAGQPDDYLEKALRDYKSGKRKNPLMAPQAQNLSKQDIKDLAAYFSSL
                     SGDLKVKY"
     gene            complement(1613..1939)
                     /locus_tag="VN496_01875"
     CDS             complement(1613..1939)
                     /locus_tag="VN496_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF15717.1"
                     /translation="MRIRIVALACLSALLPLLAAPLHAAEGDAAAGKNKTAMCAGCHG
                     IAGFRTAYPETYHVPKLGGQNAGYIVSALKAYKAGDRQHPTMKAIAASLSDQDMADLA
                     AYYSGK"
     gene            complement(2054..2884)
                     /locus_tag="VN496_01880"
     CDS             complement(2054..2884)
                     /locus_tag="VN496_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HXF15718.1"
                     /translation="MTMFLRAGSMVVAALLATLCFANEKSEPNPALSPYWDPIKQLAF
                     GDRVINPDERDVIRVYLNVRADDASTVPVVVKAQIEQTDREFIKAIYVIVERNPSPMA
                     GVFHFTPESGRAQVETRLRFEDYSFVRAVAEMNDGKLYMAQRWVKAAGGCSAPNGKKL
                     SESLLGKMKLRFDDETITYHKPNLVQLMIRHPNESPLAANFDAAQIPQFVRSVKVTYD
                     GKPVMQAEVDFSLSDNPNFRFFFTPEEKGLLNIEVEDTHDRKFSHSITISEGIPLPGG
                     "
     gene            complement(2954..3898)
                     /locus_tag="VN496_01885"
     CDS             complement(2954..3898)
                     /locus_tag="VN496_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HXF15719.1"
                     /translation="MCPVAIWAAPLVFACAAQAMPLSAQEEPVIVDLPVRQVAPGNFV
                     HYGSFEERSPQNLGDNANIGFIVGSKCVMAVDTGGSFAVGRALRQAIRRVTPLPVCYV
                     ILTHVHPDHFFGAAAFVADHPQFIAHKNYPAQLAARSRSYRNSLQRDLGEAAAGSEII
                     PPTVLVAERLQIDLGGRTVIVRAWPPAHTDDDLTVFDELSGTMWFSDLLFVRHTPVVD
                     GTITGFVAVMKELRALEFDHYVPGHGPDDLPWPQPLDAQLRYLTVILDETRAAIRNRK
                     TIQQATDEVGLSEAGHWVAFELFHRRNVTAAYAELEWE"
     gene            3967..4929
                     /locus_tag="VN496_01890"
     CDS             3967..4929
                     /locus_tag="VN496_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF15720.1"
                     /translation="MRVLPAIVYVGIALMLAGAVYGQETLQPQKVAPGVYAFVGEPGD
                     ISVANRGFVGNSGFIVGRSGVVVIDTGSSYRHGRRMLEAIARTTDKPVQLVILTHAVQ
                     EFLFGSAAFDEIGIPILAHRETAKLMQARCDHCLARLKAVLGAELEGTRLVLPRRLID
                     ATTDLDTGGRRFQLIHLGWTSTPGDLAVFDAESRVLFAGGMVSTDSIPDIRDCDFEGW
                     QRGLRQLAQLHPAHVVPGHGPVSDAGAITQTAAYLDALDSVVNALYAQSSSLMESVER
                     ATLPAYRNWAMYGSTHRQNALHRYLQLELQELGADPRSTAMPER"
     gene            5028..>5208
                     /locus_tag="VN496_01895"
     CDS             5028..>5208
                     /locus_tag="VN496_01895"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HXF15721.1"
                     /translation="MANRLSKIYTRTGDSGTTGLGDGSRVSKDSRRIEAIGAVDELNS
                     ALGVLLAEPMAENVRA"
BASE COUNT         1082 a         1535 c         1509 g         1082 t
ORIGIN      
        1 gagctgcgct cgagcgcgcc cgtgcacggc gcgcaggcac tgcttaagaa tccccgccaa
       61 cgggcttcag ccgattgcgg gggcacattt gcgataatgc cgctgatgaa ccccgccaag
      121 agagctgaaa tatttcgcag actgcgcgcc gcaaatcccg caccgaccac agaactcgtc
      181 catggcacgc cttttgaact tctgatcgcg gtgatcctgt cggcacaggc taccgacaag
      241 agcgtgaacc ttgccactgc aaaactctac gcggtggctg acacgccgca gaaaatgctg
      301 gcactaggcg aagacggaat cacgccctat atccagcgta tcggccttta ccgcacgaag
      361 gcaaaaaacg tcattgcgac ttgcgccttg ctggtggaaa agttcggcgg tgaggtgccg
      421 tccaaccggg tcgatctgga aagcttgccc ggcgtggggc gcaagactgc gaacgtgatc
      481 ctgaataccg ctttcggtca gccaacgatt gcagttgata cccatatctt ccgcgttgcg
      541 aaccgtaccg gtatcgcccc gggcaagaat gtcaccgaag tggaaaaccg gctgctgaag
      601 tttgtgcccg gggagttcct gcagaatgtg caccactggc tgatcctgca cgggcgctac
      661 gtgtgtaagg cacagcggcc ggattgtccg gcctgcctga ttcgcgatct gtgcgagttc
      721 aaagccaaga ccaaagcaga tgttcaatcc gtcgcgcgct gatgtacgcc gatttttttt
      781 cgaaacctgg cgaaaatatc gcttgcatct gccgctcgaa gggctggagc gcactgcaat
      841 tgaaatcatt ctgatgcatc ccgagcatta tccgcttctc gataatccgg agcgtaacct
      901 ggagactgag ttcacaccgg aggacggcgc agtcaacccg ttcctgcatc tgtcgctgca
      961 tcttgcgatt gccgaacagt tgtccatcga tcagccgcgg ggcattgcag ttgcttatcg
     1021 cgaacttgcc ggccggaccg catcaccgca cgatgcgttg cacgcgctgt tggaatgcct
     1081 gggcgaaatt gtctggctgg cgcagcgcgg tggaacggcc ccggacgagc tggcgtacct
     1141 ggactgtatc cgccgcactc tgcgttgagc gcagagtgcg gcccacacaa cgacggccgg
     1201 aaaaaagcaa aaggagccaa agctcctctg cttgaaatcg atgcccgtgc ggcttagtac
     1261 ttgaccttga ggtcgccgct cagactggaa aaatacgcgg ccagatcctt gatgtcctgc
     1321 ttggaaagat tctgtgcctg cggggccatc agcgggttct tgcgcttgcc tgacttgtaa
     1381 tcccgcagcg ctttttcgag atagtcgtcg ggttgcccgg cgagtctcgg gtattccggc
     1441 gcgattggag cattgccttc cgggccgtga caggtactgc acagaccggc tttctccttg
     1501 cccttggtgg catctccacc agccatggca tggccggttg caccgcatgc caccagcgcg
     1561 attatcgcaa tcaacatgtt tttcatagct tccctttttt gcgaccgagg actcatttgc
     1621 ccgaataata tgcagccagg tctgccatgt cctgatcgct caggctggcc gctatcgctt
     1681 tcatggtcgg gtgctggcga tcccctgcct tgtaagcctt cagcgcgctg acgatataac
     1741 ctgcattctg accgccgagt tttggcacgt gataggtttc cggatacgcg gtgcgaaacc
     1801 cggctatgcc gtggcaaccg gcgcacattg cagtcttgtt cttgccggcc gcggcatccc
     1861 cttccgccgc atgcagcggc gccgccagca ggggcagcag ggcagacagg caggccagcg
     1921 cgacgatgcg gattctcatg gcttgaatat ccctggtgaa cttttgaatg cggatcgctt
     1981 attgctatcg aaaaaccgcg caactctatc cgaagcgcga gggccggtca acgccggcac
     2041 gcgagagtgc ggttcaccca ccgggcaagg gtatgccttc cgatatggtg atcgagtgac
     2101 tgaatttgcg gtcatgagta tcttcgactt cgatgttcag caaccccttt tcttcgggtg
     2161 taaaaaagaa tcggaaattg ggattatccg acagcgagaa atcgacttct gcctgcatca
     2221 ccggcttgcc atcataggtt accttcacgc tgcggacgaa ctgtggaatc tgagcggcat
     2281 cgaagttcgc ggcgagcggt gactcgttcg gatgacgaat cataagctgc accaggtttg
     2341 gcttgtgata cgtgatcgtt tcatcatcga accgcagttt cattttgccc agcagcgatt
     2401 cggacaattt tttgccattc ggcgcggagc agcctcctgc cgctttgacc cagcgctgcg
     2461 ccatgtacag cttgccgtca ttcatttccg ccactgccct gacgaagcta tagtcctcga
     2521 aacgcagccg cgtttcgacc tgtgcccgcc cgctctcggg cgtgaaatgg aaaactccgg
     2581 ccatgggcga gggattgcgc tcaacaatca cataaatcgc cttgatgaat tcccgatccg
     2641 tctgctcgat ctgcgccttg acgacaaccg gaaccgtgga agcatcgtcg gcccgcacat
     2701 tcagataaac ccggataacg tctcgttcat caggattgat aacccggtca ccgaacgcga
     2761 gctgcttgat cgggtcccag tacgggctca atgcgggatt gggttcggat ttctcattgg
     2821 caaagcacag ggtcgcgagc aaggccgcaa cgaccatcga gccggcccgt aaaaacattg
     2881 tcattgcatc taccctattg ccaacgttca cgcagtatac gccgtcacgg ttcgcgtgct
     2941 gttttcggcc gcatcattcc cattccagtt cggcgtaggc cgcggttaca ttgcggcgat
     3001 gaaacagctc aaaagccacc caatgtccgg cttcggacag tccgacctca tcggtcgctt
     3061 gctggatggt ttttcggtta cggatagcag cgcgcgtttc atcaagtatc acggtcaaat
     3121 agcgcaattg cgcgtccagc ggttgcggcc agggcagatc gtccgggcca tgacccggta
     3181 cgtaatggtc aaactcgagg gcgcgtaatt ccttcatgac cgcaacaaat ccggtaatcg
     3241 taccgtcgac cacgggtgta tggcgcacga acagcaggtc cgagaaccac atggtcccgc
     3301 tcagctcgtc gaatacagtc aaatcgtcat cggtatgcgc cgggggccag gcgcgcacaa
     3361 tgactgtcct gccaccgagg tcgatctgca gccgctccgc gaccagcaca gtcggcggga
     3421 taatttccga tccggccgct gcttcgccca aatcgcgctg cagcgaattt cggtaagagc
     3481 gtgagcgtgc tgcaagttgc gcgggatagt ttttgtgcgc gatgaactgc ggatggtctg
     3541 caacaaaggc ggccgcgccg aaaaaatgat ccggatgcac atgtgtcaga atcacatagc
     3601 acactggcaa cggcgtaacg cggcggatag cctggcgcag cgctcggcct accgcgaatg
     3661 aaccgcccgt atccaccgcc atgacgcatt tactgccaac gataaaacca atattggcgt
     3721 tatcgccaag attttgtggc gaacgctcct cgaaactgcc gtagtggaca aagtttcccg
     3781 gagcgacctg gcgcaccggc aagtcgacga tcaccggctc ttcctgtgcg gacaatggca
     3841 tcgcttgcgc ggcacaagcg aacaccaagg gcgcggccca tatcgcgact ggacacatgg
     3901 ccacatctta gaacaaaaac gctattggac ttgccgcggc tggccgcctc gtttacggta
     3961 agcccgatgc gcgtccttcc tgcaatcgtt tatgtcggta ttgcgctcat gctcgccggc
     4021 gcggtgtatg ggcaagaaac gctgcagccg cagaaagtag cgcctggcgt ctatgcgttc
     4081 gtcggcgagc ccggggacat ttcggtggct aatcggggct tcgtcggcaa cagcggtttt
     4141 atcgtgggcc gctctggcgt ggttgtaatc gacaccggca gttcgtaccg gcacggccgt
     4201 cgcatgctcg aagccattgc gcgcaccacc gacaagccag tgcagctggt tatcctcacc
     4261 cacgccgtgc aagagttcct gtttggaagc gctgcatttg atgagatcgg cattccgatc
     4321 ctggcccacc gggaaacagc caagctcatg caggcgcgat gcgaccattg ccttgcgcga
     4381 ctaaaagcgg tgctcggagc agaactggaa ggcacccggc tcgtcttgcc gcggcgtctg
     4441 attgatgcca cgaccgatct cgacaccggc gggcggcgct ttcaactcat tcacctgggt
     4501 tggacaagca cacccggaga cctggccgtg ttcgacgccg aaagccgagt gctgttcgct
     4561 ggtgggatgg tttcgacaga cagcattccc gatattcgcg actgcgattt cgaaggctgg
     4621 caacgcggct tgcgccaatt ggcacagttg catcctgcgc atgtcgttcc aggccacggc
     4681 ccggtatcgg atgcaggagc gatcacgcaa acggcggcgt atctggatgc actggacagc
     4741 gtggtgaatg cgctctacgc gcagagttcg agtctgatgg aatcggtgga aagagcgacg
     4801 ctgccggctt atcgcaactg ggccatgtat ggcagcacgc atcggcagaa tgcactgcac
     4861 cgctacttac agttggaatt gcaggaactg ggtgccgacc cgcgttccac ggccatgccc
     4921 gagcgctagc gcgcgtgggc aaacgcatcg gcaaatgcat cggtatggca ccgtgtaata
     4981 ttgccttatg cattacccgc acagacaatg gcccgaccgg tgcctgaatg gctaaccggc
     5041 tcagcaaaat atatacacga accggggatt ccggaaccac tggccttggc gacggttcac
     5101 gcgtgtccaa ggacagcagg cgaatcgaag cgatcggcgc ggtggacgaa ctcaacagcg
     5161 cgctgggggt gttgcttgct gaaccgatgg cggagaacgt gcgggccg
//
LOCUS       DATMZQ010000053         3831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4507707, whole genome shotgun sequence.
ACCESSION   DATMZQ010000053 DATMZQ010000000
VERSION     DATMZQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4507707"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1512)
                     /locus_tag="VN496_01900"
     CDS             complement(151..1512)
                     /locus_tag="VN496_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HXF15722.1"
                     /translation="MMRCTQTLSAIVFSLAALVLAVAAQSVFPTGTTIYDPSRAWSGF
                     TVLSPLGTPAAIVIDMNGNVVKQWSGFVNSAGGPARIFPNGVVMAANGNNPPHQESLE
                     LVERDFNGNVLWRFDHNQQIQTNTGSTVWATRQHHDWQRDDFPAGYYSPGVQPAIQGS
                     KTLLLTHTNHTRSEVSPAALEDDRLIEISSNGDILWEWLASDHIDEFGFSAAAKSAIK
                     NTGGGGRGGGFDWLHINSATYVGPNHWFDEGDQRFAPNNVIISSRQASFLAIIARNGK
                     VVWRLGPDFSESKELRAIRQIIGQHHAHIIPKGLPGAGNVMVFDNGGASGYGFPNPTA
                     PDGSNSFARATSRVLEINPVTLELVWSYAAARFYSSNISGAQRLVNGNTLVTDGPNGR
                     LFEVTRDSKVVWEYINPQYTGANGANSVYRAYRLPYDWIPQLERPKEKAVAPPPQANF
                     RVP"
     gene            complement(1566..2117)
                     /locus_tag="VN496_01905"
     CDS             complement(1566..2117)
                     /locus_tag="VN496_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF15723.1"
                     /translation="MINGKATTEPLQCLTGHRGTDHDAGALATYFKESATMKVHLYLF
                     GMVLGVVLAVHLAMNGKVGAAINNPRVGNALFWCIGAVGALAIGLSGWRQGALDPIKG
                     VHPLLLTAGLFGATLVFAIAWLIPEAGASTVMLTLIAGQVLAGMVMSHYGMLGSPVQT
                     VSTTNLIGAVLLFAGVVLATRPS"
     gene            2293..2685
                     /locus_tag="VN496_01910"
     CDS             2293..2685
                     /locus_tag="VN496_01910"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXF15724.1"
                     /translation="MKLKQSVLFAAMIAALSATSALAHHGTATSYDQTKTLTVKGTIS
                     QFLWRNPHTALFVAVKDEQGKTSEYAVEMWSPSLMVKQGYARTTFKVGDQITLEVHPS
                     LAGKPVGECLGCKLLINGKDPRNNRTAP"
     gene            2682..3431
                     /locus_tag="VN496_01915"
     CDS             2682..3431
                     /locus_tag="VN496_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15725.1"
                     /translation="MKPVIALIYCILFASAATAQVQKAPADINGIWVRGGGGAGGRGS
                     AVSQWSPEQLPFTPEGLKQLNANKPGKGPRAVPPAFGNDPMGGANPPGLYRTLIYNRP
                     FEIIQFPNRVVQLFEWAKVWRVIWTDGRAVPDDIAEGPFWYGHSVGKWEGDTLVVHTL
                     ALDERAWLDEWGIPYSSEAKFEERWRRTAPDKLELTLKVTDPVYYTKPWTSDAKVYSL
                     QPKGELIEMIFAPIDEKEFNDRVRDPAAGKD"
     gene            complement(3573..>3831)
                     /locus_tag="VN496_01920"
     CDS             complement(3573..>3831)
                     /locus_tag="VN496_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15726.1"
                     /translation="ERPEVSFSLTEMAKDSWVVFELPGFANATSGKQQDSIDALRKAT
                     ETSYFKGPDAVWVKLVIPTAPKGIGMDQFNSGTQASITVRR"
BASE COUNT          844 a         1081 c         1075 g          831 t
ORIGIN      
        1 gcgtctctgc cgtgatggtg aagcgatgtc gccgcaccgg gcgctggaac atattcccaa
       61 atcaccacgc gttcgctttc gcgcacttgt ttccccggcg cactggggaa tgaaggcaag
      121 ctaacgtctt tgctttccaa atcaagtttc ttacggcacc ctgaaattcg cctgcggtgg
      181 tggcgcgacg gccttttctt ttggccgttc gagttgcgga atccagtcgt agggcagccg
      241 ataggcacgg tagacagagt tcgccccgtt tgcgccggta tattgcggat tgatatattc
      301 ccagacgacc ttactgtctc tggtaacctc gaacagccga ccattgggtc catcggtgac
      361 cagcgtgttg ccattcacca atcgctgcgc gccgctgatg ttggaactgt agaatctcgc
      421 tgccgcatag gaccatacga gttccagtgt caccggatta atttcgagaa cgcgagatgt
      481 cgcgcgcgca aatgaattgc ttccgtctgg agctgtcgga ttcgggaagc catagccgct
      541 ggcgccgcca ttatcgaaca ccatgacgtt gccggcgccg ggcaggcctt tcggaatgat
      601 gtgcgcatgg tgctggccga tgatctgtcg gatggcgcgc agctctttgg attcgctgaa
      661 gtccgggccg aggcgccaca ctaccttgcc gtttcgcgcg atgatcgcca gaaaactggc
      721 ctgacggcta ctgattatga cgttgtttgg ggcgaagcgc tggtcccctt cgtcgaacca
      781 gtgattcggt ccaacatatg ttgccgaatt gatgtgcagc caatcaaacc caccgccgcg
      841 tccgccgcct cctgtgttct taattgcact tttcgcagca gcggagaagc cgaattcatc
      901 aatgtgatcg ctcgccagcc attcccagag gatgtctccg tttgatgaga tctcaataag
      961 ccggtcgtct tcaagcgcgg cgggagacac ttcacttcgg gtgtggttgg tgtgtgtcag
     1021 taatagcgtc ttgctgccct gaatcgcagg ctggacgccg ggcgaatagt agccggccgg
     1081 aaagtcgtcc cgctgccagt cgtgatgctg gcgcgttgcc catactgtgc tgccggtgtt
     1141 ggtttgaatt tgctggttgt gatcgaagcg ccacagcaca ttgccgttga aatcgcgttc
     1201 gacgagttcg agagattcct gatgcggtgg gttgttccca ttggctgcca tgacgacgcc
     1261 atttggaaaa atgcgagccg gaccgccggc agaattgacg aagccactcc attgcttgac
     1321 aacgttgcca ttcatatcga tcacgatggc ggccggggtc cccagcggcg agagcaccgt
     1381 aaaaccgctc catgcacgac tcgggtcgta aatcgtcgtg cccgtcggaa aaacggactg
     1441 tgctgcaacg gccaaaacga gtgccgccag gctgaaaacg attgcgctaa gtgtttgagt
     1501 acatcgcatc atggcatcgc tcctgatttt atccacggca ttatatgacc ggtcgagatg
     1561 gatagctaac tcggccgtgt cgccagaaca acacctgcga acagcagcac cgcgccgata
     1621 agatttgtcg tactgacggt ttggacaggc gatccgagca ttccgtaatg agacatcacc
     1681 atgcctgcca aaacctggcc cgcgatcagg gtcagcatca cggtcgacgc acccgcctcg
     1741 ggaatcagcc aggcgatggc gaagacgagc gtcgcgccga agagacccgc cgtcagcagc
     1801 agaggatgga caccctttat cggatcgagc gcaccttgcc gccatccgct cagcccgatc
     1861 gctaacgccc cgacagcgcc aatgcaccag aagagcgcat tgcccacacg aggattattg
     1921 atcgctgcgc ccaccttgcc gttcatcgca aggtgcacgg caagtacgac tcccagcacc
     1981 atcccgaaca ggtacaaatg aactttcatg gttgcactct ccttgaaata tgtcgctaaa
     2041 gctccggcat catgatccgt tcctcgatgc cccgtcaagc attgcagggg ttcggtcgtg
     2101 gctttgccat tgatcatatg tttggggact cagtcaaaaa tatggaacgc atcattccgg
     2161 tccactacgc gggggataac cggattgtca cgctggccga ggtccgccgc gtcgccggcc
     2221 ggaattgaaa gctccagcga gtcgttgtgg tttattattc aatctaaatt ccggttcatc
     2281 aaggagcaat aggtgaagct gaaacaatcc gtcctgttcg ctgcgatgat cgcagcacta
     2341 tcggcgacgt ccgcacttgc acatcatggg accgcaacgt cgtacgatca gacaaagacc
     2401 ctgaccgtaa aaggaaccat ttctcaattc ctgtggcgaa atccgcacac ggctctgttt
     2461 gtcgccgtta aggacgagca aggcaagacg tcggaatatg ccgtcgaaat gtggagccca
     2521 agcctgatgg tcaagcaggg ctacgctcgg acgacgttca aggtcggcga tcaaatcacg
     2581 ctggaagttc atccttccct tgctgggaaa ccggttggtg aatgtctggg gtgcaagctc
     2641 ctgataaacg gcaaggatcc ccgcaacaac aggactgcac catgaagcct gttattgctt
     2701 tgatctactg cattcttttc gcaagtgccg cgacggcaca agtccagaag gcgccagccg
     2761 atatcaatgg catctgggta cgcggaggag gcggagccgg cggacggggc tcggcggtca
     2821 gccagtggag tcctgaacaa ttaccattca cgcccgaagg tctcaagcaa ctcaatgcca
     2881 ataagcccgg aaagggaccg cgcgccgttc cgccggcctt cggaaacgat ccgatgggcg
     2941 gtgccaatcc tccggggctt taccgaactt tgatttacaa tcggccgttc gaaatcattc
     3001 aatttccgaa tcgtgtggtg cagcttttcg aatgggctaa agtctggcgc gtcatctgga
     3061 ctgatggccg cgcggttcca gacgatattg cggaggggcc gttctggtat ggtcactcgg
     3121 tgggtaaatg ggaaggcgat accctcgtcg ttcataccct cgcccttgat gaacgggcat
     3181 ggctcgatga atggggaatc ccttacagta gtgaagccaa attcgaagaa cggtggcgtc
     3241 gtaccgcacc ggataagctg gaactcacgc tgaaagtcac cgatccggtg tattacacca
     3301 agccatggac aagcgatgcg aaggtgtact cgctgcaacc caaaggcgaa ctgatcgaaa
     3361 tgatctttgc acccatcgac gaaaaggaat tcaacgaccg cgttcgcgat cctgctgccg
     3421 gaaaagacta gaggcacagc tccgaaggag ggacacctcc aatctgagtc catctcaacg
     3481 tcgcgaaaga tcgattttga acggactatc cgccgcacaa aaaaagcccc cggcttgcgc
     3541 agggggcttt tttcagttcg cgaataaggt gcttatcgcc ttaccgtaat gctggcttga
     3601 gtacccgagt taaactgatc catgccaatg cccttggggg cggtcgggat caccagtttc
     3661 acccagacgg catccggacc cttgaaataa gaagtctcgg tcgccttgcg cagggcgtcg
     3721 atgctgtcct gctgcttccc ggaggttgcg ttggcgaaac ccggcagctc gaacacgacc
     3781 catgagtctt tggccatttc ggtcaagctg aacgaaactt ccggtctttc g
//
LOCUS       DATMZQ010000054         4697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_711099, whole genome shotgun sequence.
ACCESSION   DATMZQ010000054 DATMZQ010000000
VERSION     DATMZQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711099"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VN496_01925"
     CDS             <1..598
                     /locus_tag="VN496_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HXF15727.1"
                     /translation="AIEHCRAGIGVWQWASNDESGEPDVVMGCCGDVPTLETLAAVDF
                     LRQQLPDLKIRVINVVDLMTLHPQSEHPHGLSDETFDRMFTRDKPVIFAFHGYPVLIH
                     RLTYRRSNQERIHVRGFKEKGTTTTPFDMVVLNDLDRFHLAADVIERVPVLGERAVLA
                     LQVLRAELLNHKAYIEKHGEDLPEIRNWKWPGAVSGAC"
     gene            complement(619..1548)
                     /locus_tag="VN496_01930"
     CDS             complement(619..1548)
                     /locus_tag="VN496_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXF15728.1"
                     /translation="MAAETTQSVVAAIVGNLLVTATKLVAAAFSGSSAMLSEAIHSLV
                     DSGNGVLMLYGMRRSLKPPDEHHPLGYGHELYFWTLVVGILIFGLGGGMSIVTGIMHM
                     IGGKAPEAAWWSYAVLGAAGIFEGSSWYFGWKAFRKEQRQGRGIMETIVVSKNPTTFS
                     VLLEDTAALLGLVLAFAGIYFSALFNLPWLDGAASVLIGVLLCVSAVIMVYESMGLLV
                     GEGMEPATLAELRRIVAADPAVQQVNHLLTLYLGPEDVLLAIDLRFRPDTDVHDIRAA
                     VARFRKAIQDRYPRIRRICLDTTLIGITPPPSV"
     gene            1753..2289
                     /locus_tag="VN496_01935"
     CDS             1753..2289
                     /locus_tag="VN496_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXF15729.1"
                     /translation="MSLDIRWLIPAAIYAVAPQCMAAKYMSVDQAMEQIFPFADEYVT
                     KALQLTTEQTQEIAKLSRVAGHTGQPQVWQAMAKGRLIGWFFVDQVIGKHELITYALG
                     VNSDGSVRQLQIIEYLETYGSQVRYPNWRDQFVGKTVKNPLQIDSDIDNITGATLSAR
                     HLTDGIRRLLFLHQSVLR"
     gene            complement(2358..2726)
                     /locus_tag="VN496_01940"
     CDS             complement(2358..2726)
                     /locus_tag="VN496_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15730.1"
                     /translation="MKRLLIGLLLACANTAVAFAQGPPDVNVFSFADTSCAEWALSKN
                     NELSRAIYEAWFRGFVSGYNFGNSANQVALDKMPDRVALALYIDKYCRDNPKLPFIGA
                     AIPLVQEIREVRSVVPARRP"
     gene            3124..>4697
                     /locus_tag="VN496_01945"
     CDS             3124..>4697
                     /locus_tag="VN496_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HXF15731.1"
                     /translation="MIWLAQQAKPDREPASAGRSPPANQNLLTKGTALRNIPPRRAWL
                     TFVIILVVNYFLARLLFPNPDAPITVPYTIFKEEAAKGNVKAIYSKGASIEGRFAKPV
                     TWPAAGENTAVPGHAAQIEPRTGDTFITELPAFVDPGLETFLINQHVEISAVPIRSGN
                     SLGSLLLGFGPAILIIGFYVWLYRRAQQGGGMGGALMGIGKSKARRYDQEAGTRVTFD
                     DVAGIDEAENELVEIVDFLKAPAKYTRLGGSPPKGILLIGAPGTGKTLLAKAVAGEAG
                     VPFFSMSAAEFVEMIVGVGAARVRDLFKQAREHAPAIIFIDEIDSIGRSRGQIALGGS
                     SEQEQTLNQILTEMDGFSGREGVIVLAATNQPEVLDKALLRPGRFDRRVVVNLPDKTG
                     REAILKVHTRKVPLANDVDLSRLAAATPGLSGADLNNLVNEAALLAARREEDQVRDKD
                     FLDALEKIVLGPERPLLLSREDRERIAYHESGHAILGLVVPGADPVHRVTIVPRGQAL
                     GVTYQRPQSDRYNYPE"
BASE COUNT         1045 a         1339 c         1377 g          936 t
ORIGIN      
        1 cgcgatcgaa cactgccgcg ccggaatcgg tgtctggcaa tgggccagca acgacgaaag
       61 tggtgagccg gatgtagtca tgggctgttg cggcgacgtg ccaacgctcg agacgctggc
      121 ggcggtcgac ttcctgcgcc agcagcttcc ggatttgaag attcgcgtca tcaacgtggt
      181 cgatctgatg acattgcatc cgcagagcga gcatccccac ggattgagtg acgagacctt
      241 cgaccgcatg ttcacccgcg acaagccagt gatattcgcg ttccacggct atccggtcct
      301 gatccatcgc ttgacgtatc ggaggtcaaa tcaggagcgc attcatgtgc gagggttcaa
      361 ggagaaaggc acgaccacga cgccatttga catggtggtg ctgaacgatc tggaccgttt
      421 tcacctggcg gcggatgtga tcgagcgcgt gccagtgcta ggcgaacgcg cggtcctggc
      481 actgcaggtg ctgcgcgcgg aactgctcaa tcacaaagcg tacatcgaaa agcatggcga
      541 agacctgccc gaaatccgca actggaaatg gccaggggct gtttcaggcg cttgttaggt
      601 cgcggagtca caagctactc aaactgacgg cggcggcgtg atgccaatca acgtggtgtc
      661 caggcaaatg cggcggattc tcggatagcg atcctgaatc gcttttctga aacgggctac
      721 tgcagcgcgg atatcgtgca catcggtatc cgggcgaaaa cgcaaatcga tcgcgagcag
      781 gacgtcttcc ggaccgagat acagcgtcag caaatggttt acctgttgca ccgccggatc
      841 cgcggccaca atcctgcgca attcggccag cgtggcgggc tccatgcctt cgccaaccag
      901 cagtcccatg ctttcgtaca ccatgattac ggcactgacg cagagtagta cgccgatgag
      961 cacggaagcc gcaccgtcca gccagggaag gttgaacaac gcactgaaat aaatgccggc
     1021 gaaagcgaga acgaggccca gcagcgcggc ggtgtcttcc agcagcacgg aaaaagtcgt
     1081 cgggttcttg ctaaccacga tggtttccat gatgccgcgt ccctggcgct gttccttgcg
     1141 gaatgccttc cagccgaaat accagctgct tccttcgaat atgccggccg cacccagcac
     1201 tgcataactc caccatgcgg cctcgggcgc ttttcccccg atcatatgca tgatgccggt
     1261 gacgatcgac atgcctccgc ccagcccgaa aataagaatg ccgaccacca gagtccagaa
     1321 gtagagctca tgtccgtaac cgagcggatg atgctcgtcc ggcggcttaa ggctgcggcg
     1381 catgccatag agcatcagca cgccgttgcc agaatccacc agcgagtgaa tcgcttccga
     1441 taacattgcc gaactgccgg agaacgcagc tgcgactaac ttcgtggcag tgacaagcaa
     1501 attaccaacg atggccgcga ctacggattg tgttgtttca gcagccatgt atatcccctg
     1561 atcaatatac gtaatgtcgg tacgcgtgat tgcgtgcccg gcacgggatc gttccgcatt
     1621 ccggtggacc atgcggcaat tccgcgcccg gtcactgctt cgcagtctgg ccgagctacg
     1681 ttgtgcgata ctgcccatca attccggatc gctgtcattt ctccttagaa cacttcagat
     1741 gcataactcc gcatgagtct ggatatccgc tggctcattc cggcggctat ttacgccgtg
     1801 gcgccgcaat gcatggctgc gaaatacatg agcgtggatc aggcgatgga gcagattttt
     1861 ccgttcgctg acgaatacgt cacaaaagcg ctgcagctta caacggagca gacgcaggaa
     1921 atcgccaaac tttcgcgagt agcaggacac acggggcaac cgcaagtctg gcaagcaatg
     1981 gcgaaaggca ggctgatcgg atggttcttc gtggatcagg tcatcggcaa gcatgaattg
     2041 attacgtacg cgcttggcgt aaacagcgac ggcagcgtgc ggcagttgca aataatcgag
     2101 tacctcgaga cgtacggctc ccaagtgcgc tatcccaatt ggcgcgatca gtttgtgggc
     2161 aagacggtca aaaatccgct gcaaatcgat agcgacatcg acaacatcac gggcgcgacg
     2221 ctttccgctc gccatttgac cgatgggatc aggcgccttt tattcctcca ccaatcggtc
     2281 ttgcgctgac agtttgcagc gattgccgcc cgttgtctct tccgatcggc aaccggccag
     2341 catgcggctg gattggatca aggcctgcgc gccggtacca ctgatcggac ttcgcgaatt
     2401 tcctgcacca gcggaattgc cgcgccgatg aagggaagct tcggattgtc ccggcaatat
     2461 ttgtcgatat agagcgccag cgcaaccctg tctggcattt tgtccaaagc cacctggttc
     2521 gcggaattgc cgaagttgta gcccgagaca aagccgcgga accaggcttc ataaatggcc
     2581 ctggataatt cgttattctt ggacagggcc cactccgcgc atgaagtgtc tgcgaagctg
     2641 aaaacgttta cgtcgggcgg cccctgagca aaggcaactg ctgtgttagc gcaggcaagg
     2701 agcagtccaa tcaacagccg cttcactgca acccagcgtc tcgtccaatc gatgcctggc
     2761 ttcctcggag aagcaaatga cgtccggaat gccggtgcat ctgcatacgg catgccaggc
     2821 gaatttacga tgaaagctgt tcctcttaac atattttcgt agctggatag gaaactacat
     2881 gcggagcggc aaaaaaagca ttggaccaag caggtcttcg cgctaaacgt atcacaaaag
     2941 aatattacgg ctgtgcacaa gccatgatcc gatgttcgcc aacgaagccc ggtttgttga
     3001 aacctccggt ggcaaggaac tggtgcgcgg cacgggcggt cttactttgc cgcgccacgg
     3061 atcgtgacca tccgcgtcca gctctttggg ccaatggcgt ggacatcgac ttggggacat
     3121 tcaatgatct ggctggcgca gcaggcaaaa cccgatcgcg agccggcttc ggcagggcgc
     3181 tctccacccg caaaccagaa tttactcacc aaaggaaccg cgttacgcaa tattccgccg
     3241 cgcagggcct ggttgacgtt cgtgatcatc ctggtcgtga actattttct ggcgaggctc
     3301 ctgtttccga atcctgacgc gccgatcacc gtgccgtata cgattttcaa ggaggaggcc
     3361 gcgaagggta atgtcaaggc catctacagc aaaggagcga gcatcgaagg gcgtttcgcg
     3421 aagccggtca cgtggcccgc ggctggcgag aacactgcag tgcctgggca cgctgcgcaa
     3481 attgagccgc ggaccgggga taccttcatt accgaactgc cggcatttgt cgatccaggc
     3541 ctggagactt tccttatcaa tcagcacgtc gaaatcagcg cggtgccgat tcgcagcggc
     3601 aactcattgg gaagtttgct gctcggattc ggtccggcca ttctgattat cggtttttac
     3661 gtctggcttt accggcgggc tcagcaaggt ggcgggatgg gaggggcatt aatgggcatc
     3721 ggcaaaagca aagccagacg ctatgaccag gaagccggga ccagagtcac cttcgacgac
     3781 gtagcgggca tcgatgaagc cgagaatgaa ttggtggaaa ttgtggattt cctgaaggcg
     3841 cctgcgaagt acacccgcct tggcggcagc ccgccgaaag gcattctgct gatcggcgca
     3901 cctggaaccg gcaaaacgct gctggcaaaa gccgtcgcag gggaagccgg ggtgccgttt
     3961 ttttccatga gtgcggctga attcgttgag atgattgtcg gcgtgggcgc tgcgcgagtg
     4021 cgcgatctgt tcaagcaagc gcgagaacat gcacccgcga ttattttcat cgacgagatc
     4081 gattcgatcg gccgctcgcg cggccagatc gcgctcggcg gttcgagcga acaagagcag
     4141 accttgaacc agatactgac tgaaatggac gggttctccg gtcgcgaagg cgtcatcgtg
     4201 ctggcggcga ccaatcagcc ggaagtgctg gacaaagcgt tgctgcggcc cggccgcttt
     4261 gatcggcgtg ttgtcgtcaa tcttccggat aagaccggac gggaagcgat tctgaaagtg
     4321 catacccgca aagtgccgct ggccaacgat gtcgatctct cgcgactggc ggcggcaacg
     4381 ccagggcttt ccggcgccga cctgaacaat cttgttaacg aggccgccct gctggccgca
     4441 cgccgggagg aggaccaggt acgcgacaaa gatttcctcg acgcactgga aaagatcgtg
     4501 ctcggacctg agcgtcctct tttgctcagc cgcgaagatc gtgaacgcat tgcctatcat
     4561 gaaagcggac acgcgatcct cggcctggtc gtgcccggtg ccgatccagt gcaccgggtc
     4621 accatcgtgc cgcgcggaca agcgctcggc gtgacgtatc agcggcctca gtcggatcgt
     4681 tacaactacc cggaaaa
//
LOCUS       DATMZQ010000055         2756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2803481, whole genome shotgun sequence.
ACCESSION   DATMZQ010000055 DATMZQ010000000
VERSION     DATMZQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803481"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..775
                     /gene="msrB"
                     /locus_tag="VN496_01950"
     CDS             284..775
                     /gene="msrB"
                     /locus_tag="VN496_01950"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXF15732.1"
                     /translation="MNRRQLLASIPALFIGLPVLARENSAGTLVKTKAEWKTLLPETS
                     YRVLFEEDTERPGSSPLNKEKRAGTFVCVACYLPLFGADTKYDSGTGWPSFFDHLPGA
                     LGTKKDRTLIWTRTEYHCARCGGHQGHVFDDGPQPTGLRYCNNGVALRFVPKEEKLPA
                     LRT"
     gene            complement(865..1344)
                     /locus_tag="VN496_01955"
     CDS             complement(865..1344)
                     /locus_tag="VN496_01955"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXF15733.1"
                     /translation="MNRIPCCVSIVYLCFAFSHNVHAQRESNDERVQPEYLTSPATTV
                     LKRPFSEAVRVGDMLYLSGFTGRIPGTNNVVPGGIQAETKQVMENIKGALDRYGSDFE
                     HIVKCTVMLLDISDWAAMNEVYRTYFPADRMPARSAFATNGLAFKARVEIECLATMR"
     gene            complement(1447..2145)
                     /locus_tag="VN496_01960"
     CDS             complement(1447..2145)
                     /locus_tag="VN496_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HXF15734.1"
                     /translation="MAAANAQPVSIEIEGGQQVSGLLQSTNRARACYILAHGAGAGMT
                     HPFMTAAANELAQRGIATLRYNFPYMDKGSKRPDSPRTAQATVRAAVAHAGRLLPLVP
                     LFAGGKSFGGRMTSQAQAAAPLPGVRGLVFLGFPLHAPGRPSDERAQHLYEIRIPMLF
                     LQGTRDEFADFSLLEPLIGKLGATASLVPFQDANHSFHVPARTGRTDAQIMSVLLDTL
                     ADWIDTVLASRNAG"
     gene            complement(2145..>2756)
                     /locus_tag="VN496_01965"
     CDS             complement(2145..>2756)
                     /locus_tag="VN496_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXF15735.1"
                     /translation="TVEINNTFYRMPNPTVLQMWAAITPEHFRFTIKAPRRITHMARL
                     KADSAADSVGYLYRCLESLGGKLAAVLFQLPPDLKKDLPRLNAFLQLLPEGHRAAFEF
                     RNDSWFADEVYEALQTAGAALCLSEREDASAPPLVQTTGWGYVRLRLETYTESDLQQW
                     AERLRGTQWSEVFAYFMHEPTAPAYAQALMQFAIVSEPVRKHA"
BASE COUNT          544 a          841 c          838 g          533 t
ORIGIN      
        1 cgggaggttt gtaagcagcg gaaacaggcc ttgccttttg cctcgctcgc gtattttcaa
       61 ctgccgccgc gtcagcgctc ggacgacgta tagtcgtgtt ccataaagcc cgccgctgcc
      121 gagcggcgcc tggttcatcg agttccgatt tgcatccggc gggcgcaagc agtcgtatat
      181 gcaacatgcg caatgcggca tcccacctgg aattgaccgg gaacgttcct gtgcgatcgg
      241 gagcccaatc tagtaaccga agtttcctgt tgaaggacta accatgaata gacgtcaatt
      301 gcttgcgagc attcctgcgc tcttcatcgg attgccggtg ctcgccaggg aaaacagtgc
      361 gggcaccctg gtcaaaacca aagcggaatg gaaaaccctg ctgcccgaga cgagctaccg
      421 ggtgctgttc gaggaagata ccgaacgccc gggcagcagc ccgttgaaca aggaaaaacg
      481 cgccggcacg ttcgtgtgcg tcgcatgtta cctgccgctg ttcggagccg acacgaagta
      541 cgacagcggc accggctggc cgagtttctt cgatcacctg ccgggtgcgc tcggcacgaa
      601 aaaggatcgg acattgatct ggacgcgcac cgaataccat tgcgcgcgct gtggcgggca
      661 tcaaggccat gtcttcgacg acggcccgca gcctaccgga ctgcgttact gcaataacgg
      721 cgtggcgctg cgcttcgtgc cgaaggaaga aaagcttccc gcgttgcgca cctgaagcgc
      781 ccggaccggg atcgctgccc cgcgtagtct gcgctcgccg cgaccgccgg aagcgctctc
      841 agccggatcc tgccacatgc gctcctagcg catcgtcgcc agacactcga tctcgaccct
      901 tgccttgaat gccaggccgt tggtcgcaaa cgcgctgcgc gccggcatgc gatcggccgg
      961 aaaataggtg cgatagacct cattcatcgc cgcccagtcg ctgatgtcga gcagcatcac
     1021 agtgcatttc actatgtgct cgaagtccga accgtaacga tcgagcgcgc ccttgatgtt
     1081 ttccatgact tgcttcgttt ccgcctggat gccgccgggc actacattgt tggtgcctgg
     1141 aatgcgcccg gtgaatccgg acaggtacag catatcgccg acccggaccg cttcggaaaa
     1201 cggccgcttc agcacggttg tggccggcga cgtcaggtac tccggctgca cccgctcgtc
     1261 gttcgactcg cgctgggcgt gcacgttgtg ggagaacgcg aaacaaaggt aaacgatgct
     1321 tacgcaacac gggatcctgt tcatggcgca ctcctgtagg cggattcagt ggtaccgcgg
     1381 atagaaaacg gggctgcatg ccctgtcgtt tattgattcg gtgttcgctt gaccgcgccc
     1441 cattagtcag ccagcgttgc gtgaggccag cacagtgtcg atccagtcgg ccagggtgtc
     1501 cagcaacacg gacattatct gcgcatccgt gcgcccggta cgcgctggca catgaaacga
     1561 gtgatttgca tcctggaacg gcacaagaga cgcagttgca ccgagtttcc cgatgagcgg
     1621 ctcgagcagc gagaaatcgg caaactcatc gcgcgtaccc tgcaggaaca gcattggaat
     1681 ccggatttcg taaagatgct gagcgcgctc gtcagacggg cggcccggtg catgcaaggg
     1741 aaagccgaga aacaccagcc cgcgcacgcc gggcagcggg gcggccgctt gcgcctgcga
     1801 cgtcatgcga ccaccaaacg attttccgcc cgcgaacagc ggcactagcg gcagcagccg
     1861 gcccgcgtgt gcaaccgcag cgcgcaccgt ggcttgtgcg gtcctcgggc tgtccggtcg
     1921 ctttgaacct ttgtccatgt agggaaagtt ataacgcagc gtggcaatgc cacgctgcgc
     1981 gagttcgtta gcggcggcag tcatgaaagg atgcgtcatg cccgcgccgg caccatgcgc
     2041 caggatataa catgcgcgcg cacggttagt tgattgcagc aggccgctga cctgctggcc
     2101 gccttcgatt tcgatgctga ccggttgcgc gtttgcggcg gccatcacgc gtgtttacgc
     2161 accggctccg atacgatggc gaattgcatc agcgcctggg cgtacgctgg cgcagtcggc
     2221 tcatgcatga aataggcaaa gacttcgctc cattgcgtgc cacgcaagcg ctctgcccat
     2281 tgctgaagat cgctttccgt gtaggtctcc agccgcaacc tgacgtaacc ccatccggtt
     2341 gtttgcacca ggggcggtgc gctggcgtct tcgcgctcgg aaagacacag cgccgcgccg
     2401 gcagtttgca gcgcttcgta aacctcgtcg gcaaaccagc tgtcattgcg aaactcgaac
     2461 gctgcccggt gtccctcggg cagcagttgc agaaatgcat tgagtcgcgg cagatctttt
     2521 ttgagatcgg ggggcaactg aaacagcacc gctgcgagct tgccgccaag cgattccagg
     2581 cagcggtaga gataaccaac cgagtctgcc gcggagtcgg ccttcaaacg cgccatgtgg
     2641 gtaatgcgcc gcggcgcttt gatggtaaac cggaaatgct ccggcgtaat cgcggcccac
     2701 atttgcagca ccgtggggtt cggcattcga tagaacgtgt tgttgatctc cacggt
//
LOCUS       DATMZQ010000056         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_712012, whole genome shotgun sequence.
ACCESSION   DATMZQ010000056 DATMZQ010000000
VERSION     DATMZQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712012"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1731
                     /locus_tag="VN496_01970"
     CDS             100..1731
                     /locus_tag="VN496_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HXF15736.1"
                     /translation="MAGFLNKERTIAGPTFNRWLVPPAALCIHLCIGMAYGFSVFWLP
                     LTKALGISKSIECPADISWVAELFTTTCDWDKPILGWMYTLFFVFLGSSAALWGGWLE
                     RAGPRKAGVVAAVCWSGGLLISAVGVYTHQLWLMWLGSGVIGGIGLGLGYISPVSTLI
                     KWFPDRRGMATGMAIMGFGGGAMIGSPLAAKLMKYYATPTSVGVWETFVTMGVIYFVF
                     MMVGALSYRVPPTGWRPSGWTPPVTAGNTMITSQHVHLRNAHKTAQFWLLWGILTLNV
                     SAGIGVIGMASPMLQEIFGGELIGLNLRFDELSKEQLGQIAVIAAAFTGLLSLFNIAG
                     RFFWASLSDYIGRKATYFVFFVLGFILYASAPWAGHAGHLALFVGFFCIILTMYGGGF
                     ATIPAYLADIFGTQMVGAIHGRLLTAWSTAGVLGPVIVNYINDYQIKHGTPKSAAYDT
                     TMYILAGLLIGGFICNLLVRPVKQKWFMTDAELEVERKLAHDAAAKSAVGGDASAVSG
                     KGSRRGLVALFWLFVGIPLAWGVWTTITNALVLFK"
     gene            1804..2718
                     /locus_tag="VN496_01975"
     CDS             1804..2718
                     /locus_tag="VN496_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF15737.1"
                     /translation="MKTILITGAAGDVGTHLRRELAGKYHLRLSDIKPVKDLAAGEQF
                     APGDIAKIAHTLRITRGVDAIVHLGGFSVEGPWDMILRANIVGCYNMFEAARRNGVKR
                     VLFASSNHATGFYRRDQKIDHCVLTKPDSRYGVSKAFGEQLGSLYADKYGMEIFCMRI
                     GNVTVAPPDKRRLSNWISPGDMARLVSVGIDHPDIKFEIVYGVSDNRRSWYDNSNAYR
                     LGYRPKDDAERFAAEVLAKEQPGDPRAELFQGGNFVFAEIGGDPSRAVPKLKVSAVQS
                     RRSTKSTGSRKPKAAQMKKPAKRSKTRR"
BASE COUNT          560 a          768 c          857 g          604 t
ORIGIN      
        1 agccaggggt ttacggtatt attggccagc accgcggtcg ccgatgcgat gcgggccaag
       61 ccgcccggca taaaaagcat aacgaggagg agccgtcata tggctggatt tctgaataaa
      121 gaacgcacca tagcgggacc aacgttcaat cgctggctgg taccgccggc ggcactctgc
      181 attcacctct gtatcggcat ggcctacggt ttttcggtgt tctggctgcc gttgaccaag
      241 gcgctgggta tcagcaaatc gatcgaatgt ccggcggaca tcagctgggt cgcagagttg
      301 ttcacgacga cttgcgattg ggataagccg atcctgggct ggatgtatac actgtttttc
      361 gtctttctcg gatcctctgc ggcactctgg ggcggatggc tggaacgcgc ggggccgcgc
      421 aaggccggcg tggtcgctgc cgtgtgctgg tcaggcgggc tgctgatctc ggcagtcggc
      481 gtctataccc accagttatg gctgatgtgg cttggttccg gcgtgatcgg tggcatcggg
      541 ctcggattgg gctacatttc tccggtatcg acgctgatca agtggtttcc cgaccggcgc
      601 ggcatggcca ccggcatggc gatcatgggc ttcggcggtg gtgccatgat tggctccccg
      661 ctggccgcaa aattgatgaa gtactacgcc acgccgacgt ccgtcggcgt ctgggaaacc
      721 ttcgtcacca tgggcgtgat ttactttgtg ttcatgatgg tcggtgcgct gtcctatcgc
      781 gtgccgccga ccggttggag gccatctggc tggacaccac cggttaccgc cggcaacacg
      841 atgatcacga gccagcatgt gcatctgagg aatgcgcaca agaccgcgca attctggctg
      901 ttgtggggca tcctgacgct aaacgtgtcg gcaggtattg gcgtgatcgg catggcctcg
      961 ccgatgctgc aggaaatatt cggcggcgag ctgatcgggc tgaatctgag attcgatgag
     1021 ctcagcaaag agcaactcgg tcagatcgct gttatcgcgg ccgcatttac cggcctgctg
     1081 agcctgttca acattgcagg acggttcttc tgggcctcac tgtccgatta catcggccgc
     1141 aaggcgacat actttgtatt ctttgtactc ggttttatcc tgtacgcgtc ggcgccgtgg
     1201 gccgggcatg cggggcacct ggcgttgttc gtcggatttt tttgcatcat cctgaccatg
     1261 tacggtggcg gctttgccac gatcccggcc tatctggcag acatcttcgg cacgcaaatg
     1321 gtgggcgcca ttcacgggcg tttgttgacg gcatggtcga ccgctggcgt actgggacca
     1381 gtcatcgtca attacatcaa tgattatcag atcaagcatg gcacgccaaa aagcgccgcg
     1441 tacgatacga cgatgtatat cctcgcgggc ttgctgatcg gcggcttcat ttgcaatctg
     1501 ctggtgcggc cggtgaagca gaaatggttc atgacggatg ccgaactcga agtggagcgc
     1561 aagctggcac atgatgcggc agcaaaaagc gcagtcggcg gggatgcatc cgcggtcagc
     1621 ggcaaaggca gcaggcgcgg tctggtcgcg ctgttctggt tgttcgtcgg tatcccgctc
     1681 gcatggggtg tatggacgac aatcacgaac gcgctggtgc tgttcaagta aggttttgga
     1741 tgccttttgg ataaccggcc cgcagaaatt ccgcgggccg ttttatttgc aaggatggaa
     1801 cgcatgaaga ccatactcat taccggcgcg gcgggcgatg tcggaaccca tctgcgccgg
     1861 gaactggccg gtaaatatca tctgcgattg tcggacatca agccggtcaa ggacctggca
     1921 gcaggtgaac aatttgctcc tggcgatatt gcaaaaatcg cgcacacact gcgcattact
     1981 cgaggtgttg atgccatcgt gcacctcggc ggtttttccg tcgaaggtcc gtgggacatg
     2041 atcttgcggg ccaatatcgt cggctgctac aacatgttcg aggccgcacg caggaacggc
     2101 gtgaagcggg tgctattcgc ctccagcaat cacgccacag gcttctacag gcgcgatcaa
     2161 aaaatagatc actgcgtgct gaccaaaccg gacagccgtt acggcgtctc caaggccttt
     2221 ggcgagcagc tgggcagcct atacgccgac aagtacggta tggaaatctt ctgcatgcgc
     2281 atcggcaatg tcacggtcgc gcccccggac aagcgccggc tgtcgaactg gatcagcccg
     2341 ggcgacatgg cacggctggt gtcagtgggg atcgaccatc cggatattaa attcgaaatc
     2401 gtctatggcg tttccgacaa ccggcgcagc tggtacgaca attcgaacgc ttaccgcctc
     2461 gggtaccgac cgaaggacga cgccgagcgc tttgccgcag aagtgttggc taaagaacag
     2521 cccggcgatc cgcgtgctga actgttccag ggtggcaatt ttgttttcgc tgaaattggc
     2581 ggtgatccga gccgcgccgt gcccaaattg aaggtatctg ctgttcaatc gaggcgaagc
     2641 accaagagca caggatcgcg caagccgaag gcagcgcaaa tgaaaaagcc ggcaaaacgc
     2701 agcaaaacca gacgctgatc ttgactgcca tcctgctcac gcatccgcct caagtccggc
     2761 agcattacta ctgcgacgag gccctcgct
//
LOCUS       DATMZQ010000057         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3840118, whole genome shotgun sequence.
ACCESSION   DATMZQ010000057 DATMZQ010000000
VERSION     DATMZQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3840118"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VN496_01980"
     CDS             complement(<1..1227)
                     /locus_tag="VN496_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF15738.1"
                     /translation="MTGATIPSFRRDAQVIGLVGLAHGTSHFFHLALAPLFPWLKEAF
                     GVSYAELGLLMSVFFIVSGVGQALAGFVVDRFGALRVLFGGIALLGISALGLAASQTY
                     VMLMFFAGVAGLGNCVFHPADFTLLNRRVSIPRLGHAFSVHGLTGTLGWAVAPLFLAG
                     IASISSWRVALAAAGVLAFTVMTVLIVCRDLLDPREVGEAVAESAKKKAAVNTTLGFM
                     KVPAVWMCFAFFFISSISFGGIQSFAATALRELYEVPLTYATACITAYMLGSAGGIVA
                     GGFLAARFQQHDRVIAVSFAAAASVSVLVASTVPPAAMVILLLGALGFGSGVAGPSRD
                     LLVRAAAPKNATGRVYGVVYSGLDIGLSGAPLMFGLLMDAHHPGWVFIGIGAFQALSL
                     IAVMGVGETASKRQIQA"
     gene            1329..2165
                     /locus_tag="VN496_01985"
     CDS             1329..2165
                     /locus_tag="VN496_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid hydroxylase family protein"
                     /protein_id="HXF15739.1"
                     /translation="MFRPISYSLPADYGRSIGHVATGLLTAKQAAFRDGYRRNIASWY
                     NGWGHLLSIFVPGVAVIWFCVAHVQQPTLWEVLFVIPVLVFYNWNEWWLHKNAMHRPI
                     KAIGGILLPVYHRHTHQHHQYFTAQVMTYDTNREWRIVLFPPYALLVFMVMTLPAALF
                     VGLLWSPNAGYILMMLTPMYYLNYETFHLCCHVHDNWFVRNCPLVNTIRRHHAAHHSQ
                     PIMMERNMNLTYPVADWYMGTSDLKRGLLGHLFNGYSTRYVKKEYAADQGAELDGRVV
                     TS"
     gene            2280..2579
                     /locus_tag="VN496_01990"
     CDS             2280..2579
                     /locus_tag="VN496_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15740.1"
                     /translation="MDLQEFTLTTSHDVPPVGLMPVLEALWYLRKGNWDRAHAVVQEH
                     EDDKAYALVHAHLHRVEGDLANAGYWYRRAGKSVSNMALEEEWQTLAVHFLREST"
     gene            2576..>3131
                     /locus_tag="VN496_01995"
     CDS             2576..>3131
                     /locus_tag="VN496_01995"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HXF15741.1"
                     /translation="MTAFNIRVDPVTQSAPPLLASPLGFGRHFTGRMFTQQYTAGRGW
                     HNAAIGPYQPLLLDPAAQVFHCGQMIFEGTKAYLRPDGNINLFRVEKNAERFNLSARR
                     LAMPAVDIDQHIQAIAELVKLERGWVPKQDGAALYIRPVMLAIENTLEVRGSHNFLHY
                     IILSPVAPYFAGGFKPVSVLVSDDY"
BASE COUNT          711 a          911 c          888 g          621 t
ORIGIN      
        1 cgcctgtatt tgccgtttgg aggcggtttc gcccactccc atcaccgcaa tcagggacag
       61 cgcctgaaaa gcgccaatgc ctatgaacac ccagccagga tgatgtgcgt ccatgagcag
      121 gccaaacatt agtggggcgc cggaaagccc gatgtccagt ccggaataca ccacgccgta
      181 gacgcggccg gtcgcatttt tcggcgcggc cgctcttacc aacaggtcgc gcgatgggcc
      241 cgccacgcca gatccgaatc caagtgcacc caatagcaag atcaccatcg ctgccggcgg
      301 aacggtgctt gcaaccagta ccgacacgct ggcagctgcg gcgaaggaaa cagcgatcac
      361 tcggtcatgc tgttggaacc gggcagccag aaaacccccg gccacgatgc cgcctgcact
      421 gccgagcata tacgccgtga tgcaggccgt cgcatacgta agcggtactt catagagctc
      481 gcgcagcgct gtggcggcaa agctttggat accgccgaac gatatagagg agatgaagaa
      541 gaaggcaaaa cacatccaca ccgctggaac tttcatgaag ccgagagtcg tgtttacggc
      601 cgcttttttc ttcgctgatt ccgccaccgc ctccccgact tcccgaggat cgagcaggtc
      661 gcggcaaacg attaataccg tcatcactgt aaatgccagc actcccgccg ctgccaacgc
      721 aacgcgccaa ctggaaatgc ttgcgatgcc ggccagaaac aggggcgcga cagcccagcc
      781 cagcgtaccg gtcagtccgt ggacgctgaa cgcatggccg aggcgcggaa tgctgacgcg
      841 acggttcagc agcgtgaaat ctgccgggtg gaatacgcag tttcccaagc cggctacgcc
      901 cgcaaagaac atgagcatta cataggtctg gcttgcagcc agacctagtg ccgaaatgcc
      961 aagcaatgca atgcccccga acagcacgcg cagagcgcca aagcggtcta ccacgaatcc
     1021 ggcaagcgcc tgtccgacgc ccgagacgat gaaaaacacg ctcatcagca gaccaagctc
     1081 cgcatagctc acaccgaaag cctctttcag ccaagggaat agcggtgcga gtgcgagatg
     1141 aaaaaaatgc gaggtgccgt gcgccaggcc taccaggcca ataacctggg cgtcgcggcg
     1201 aaatgaagga atagttgcgc cagtcatggt tttcggaggc aaattcagca cgacacctta
     1261 gagcaacgcg ttgggcgagg caaggcaggc gttgcggtgc gccttgaccc cggctacggc
     1321 gtggaggtat gtttcgccca atctcgtaca gccttcctgc ggactatggc cgcagcatcg
     1381 gacacgtggc gactggactg ttaaccgcaa agcaagcagc cttccgggac ggataccggc
     1441 gcaacattgc ttcctggtac aacggatggg gacatctgct atcgatcttc gttcccggcg
     1501 tggccgtcat ttggttctgc gtcgcgcatg tgcagcagcc aaccctctgg gaagtattgt
     1561 ttgtcattcc agtgctggtg ttctacaact ggaacgagtg gtggctgcat aaaaacgcaa
     1621 tgcatcgccc catcaaggct atcggcggca ttctgctgcc tgtctaccac cgccataccc
     1681 atcagcacca tcagtatttc accgcacaag tcatgactta cgataccaat cgcgaatggc
     1741 gcatcgtgct gttcccgcct tatgcgttgc tcgtgttcat ggtgatgaca ttgccggcgg
     1801 cgttgtttgt gggcctgctg tggtcgccga acgccggcta catactgatg atgctgacgc
     1861 cgatgtatta cctgaactac gaaacgttcc atttgtgctg ccacgttcac gacaattggt
     1921 ttgttcgtaa ttgtcctttg gtgaacacca tccgccgtca tcacgcggca caccacagtc
     1981 agccgatcat gatggaacgc aacatgaatc ttacttatcc ggttgccgac tggtacatgg
     2041 gtaccagcga tctcaagcgc ggcctgctcg ggcatctgtt caacggctac agcacgcggt
     2101 atgtcaagaa agagtacgca gcggaccaag gcgctgagct cgacggtcgc gtcgtaactt
     2161 cctgaacatc gggattccct gacaaaaaga gcctcaaggg gaagggagga tgggcgcttt
     2221 cgggcgccca tccgtttttt cggccggcaa aatgctcggt tgattcataa tggcaaagaa
     2281 tggacctgca ggaatttacc ttaactacat cccacgatgt gcccccggtg ggattgatgc
     2341 cagtgctgga agcgttgtgg tatctgcgca aaggcaattg ggaccgcgcg catgcagtcg
     2401 tgcaggagca cgaggacgac aaggcatacg cgctggtcca tgcacacctg caccgcgttg
     2461 aaggcgacct ggccaatgcc ggctattggt accgtcgtgc agggaaaagt gtctcgaaca
     2521 tggcgcttga agaagaatgg caaacattgg cagtgcattt tcttcgggag agcacttgac
     2581 cgctttcaat atccgtgtgg atcctgtaac gcagtcagcg ccgccgctcc tggcaagccc
     2641 gctggggttc ggccggcatt tcaccggccg catgtttact caacagtaca cggcgggaag
     2701 aggctggcac aacgcagcca taggacctta tcagcctctg ctattggatc ccgcagccca
     2761 ggtttttcat tgcgggcaga tgatattcga gggcaccaag gcttatcttc ggccggacgg
     2821 caatatcaac ctgtttcgcg tagagaagaa tgccgagcga ttcaatctct cggccaggcg
     2881 cctggccatg cccgctgtgg acatcgatca acacattcag gccatcgcgg aactagtcaa
     2941 gctggaacgt ggttgggtgc caaaacagga cggtgcggcg ctttatatac ggccggtgat
     3001 gcttgcgatc gaaaatacgc tggaagtgcg aggcagccat aattttcttc actacatcat
     3061 tctgagcccg gtggcgccct actttgccgg cggcttcaag ccggtgtcag tcctggtgtc
     3121 cgatgattat g
//
LOCUS       DATMZQ010000058         4979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3840244, whole genome shotgun sequence.
ACCESSION   DATMZQ010000058 DATMZQ010000000
VERSION     DATMZQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3840244"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VN496_02000"
     CDS             complement(<1..1067)
                     /locus_tag="VN496_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXF15742.1"
                     /translation="MGRPEKIRLGEILLQQQLLTADKLQSALDEQKRSGRKLGRIFVD
                     AGYVTEEQLGSALARQLQVPYVDLKRFNIRPDVANLLPETLARRFRAIVLEDAATFYR
                     VGMADPTDLFGYDQINRVLKRDIQLAVVSESLLLKTIDRVYRRTEEISGLAAELGAEL
                     GESVDLGELGLSPGAEDAPVVKLLQSVFEDAVQVRASDVHIEPQQSKVLIRFRIDGVL
                     HVQAEADSKIAAALILRLKLMSGLDISERRMPQDGRFHVKVRNIPIDVRISTMPTQHG
                     ESAVMRLLNQSGGILQLGSIGLPPGMLLRLREIIERPSGMVLVTGPTGSGNTTTLYAA
                     LEELNTTERKIITVEDPVEYRL"
     gene            1167..1631
                     /gene="trmL"
                     /locus_tag="VN496_02005"
     CDS             1167..1631
                     /gene="trmL"
                     /locus_tag="VN496_02005"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HXF15743.1"
                     /translation="MFDIVLFEPEIPPNTGNVIRLCANTGCRLHLIGPLGFRLDDRQL
                     KRAGLDYHEYASVRVHANWLDALKAIPGRKFAFSTKGINRFDSVDFRPGDAFVFGPET
                     RGLPAELLQSFDPSFRLRLPMVAHSRSLNLSNAVAVVVYEAWRQNGFSGMAS"
     gene            complement(1628..2620)
                     /locus_tag="VN496_02010"
     CDS             complement(1628..2620)
                     /locus_tag="VN496_02010"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXF15744.1"
                     /translation="MRLSILGAGAWGTALGIALSPHHEILLWARDPAQCRSLVTERIN
                     ALYLPGVVIPQHVRITPDMNLALQHGDMIVVAVPTAALRQTLKLIAASSPAAGVVWLC
                     KGFEPGTAKFAHQVAEEELSPARRRAVLSGPSFASEVARGLPAAVTLASSDNKFAALA
                     SQALHTGVFRVYSSDDVIGVETGGAVKNVIAIAAGVCDGLHLGASARAAVITRGLAEI
                     TRLGMRLGGRSDTFMGLAGAGDLVLTATSDLSRNRRVGIQLANGEGLSAILAQLGHVA
                     EGVHSASEVLHLAAQLKIDMPITAAVFELLNGSTSARDLVSELLQRGPKQESVR"
     gene            complement(2617..3081)
                     /locus_tag="VN496_02015"
     CDS             complement(2617..3081)
                     /locus_tag="VN496_02015"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXF15745.1"
                     /translation="MRRRIDSTMIVKLRLVALMGLLVSANVSALEYRSVSESAAILYD
                     APSTKAQKLFLLSRGYPVEVVVVLEGWTKVRDDTGEFAWIETVRLSDRHTVMIKVAGA
                     EARQNPNEDSPVVFTAEKGVFLELIGMATGWAKVRHADGAIGFVKVSQLWGV"
     gene            complement(3065..3529)
                     /gene="secB"
                     /locus_tag="VN496_02020"
     CDS             complement(3065..3529)
                     /gene="secB"
                     /locus_tag="VN496_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HXF15746.1"
                     /translation="MSQQPNQPVFSIEKLFVKDLSVEVPNAPKIYLERDTPQIEIQMT
                     SAANPIDEGYFEVVVSVTVTAKLAEKTVFLVEASQGGVFQIRHVPQTELEPVLGVTCP
                     NILYPYVREVISDAVVRAGFAPVVLNPVNFEAIFQAQRDARVQSAAAGATTH"
     gene            complement(3565..3843)
                     /gene="grxC"
                     /locus_tag="VN496_02025"
     CDS             complement(3565..3843)
                     /gene="grxC"
                     /locus_tag="VN496_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HXF15747.1"
                     /translation="MPHISMYCTAVCPYCIAAERLLKSKGVSDITKIRIDSQPAKRAE
                     MMERTGRRTVPQIYIDDRHVGGFEDLAALDAAGELDPLLHQAPEPTRS"
     gene            complement(3843..4253)
                     /locus_tag="VN496_02030"
     CDS             complement(3843..4253)
                     /locus_tag="VN496_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXF15748.1"
                     /translation="MKFVLNNIWLVLAALISGAILIWPLINRRLSGVPEIGAMQAVQL
                     LNRKNAVMIDVREPAEFSAGHAPNSRNIPLPQLDKRIGELEKFKNRPAVLVCQTGNRS
                     HAATTQLKKAGFAEVVVLAGGIGAWQQANLPVEK"
     gene            complement(4250..>4979)
                     /locus_tag="VN496_02035"
     CDS             complement(4250..>4979)
                     /locus_tag="VN496_02035"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HXF15749.1"
                     /translation="AAEFGAAGLILLLSGLGWWGASLRRAQIDSPLWWMLAAIAVLGI
                     HSMLEYPLWYAYFLGLFAMIMGAAERDAPAIVQGSSGRLVLCAAVVLGAFAFTNVCLD
                     YRAMQSLQGTVPAESATGSDDVSIRVLLDMQRASLFAPFIEFALARRMLLNQELLQDK
                     VVLNARAMRFQPSNDFAYRQALLLALSGDHAGMRAQWDLAVANYPSDREEALKVAQAL
                     EKSGQTGMKDLLSYAQQQNEKAKK"
BASE COUNT         1102 a         1477 c         1303 g         1097 t
ORIGIN      
        1 agcctgtatt ccaccggatc ttccaccgta atgatctttc gttccgtcgt gttcaattcc
       61 tccaaggcgg catagagggt ggtggtattg ccgctgccgg tggggccggt gaccaggacc
      121 atcccgctcg gccgctcaat gatttcgcgc aacctcagca gcatgccggg cggcaaacct
      181 atggatccga gctgcaggat accgccgctt tgattcagca aacgcatcac ggcggattct
      241 ccatgctgcg tcggcatggt tgaaatacgc acgtcaatcg ggatgttccg gacttttaca
      301 tggaaacggc cgtcctgcgg catgcgcctt tcagagatat cgagccctga catgagcttc
      361 aaccgcaaga tcagcgccgc agcaattttc gaatcggctt ccgcctggac atgcaacacg
      421 ccatcaatcc gaaagcgaat aagcaccttg ctttgctgcg gttcgatatg cacatcggac
      481 gcccgcactt gcactgcgtc ctcgaatact gattgcagca gctttacgac tggtgcgtcc
      541 tcggctccag ggctcagccc caattcaccg aggtcgaccg attcgccaag ctcagcaccg
      601 agttcggccg caaggccgct gatttcttcg gtgcgccggt aaaccctgtc gatcgtcttc
      661 aacagcaacg attcgctcac cacagcgagc tggatgtctc gcttcagaac gcgattgatt
      721 tggtcgtaac cgaagaggtc ggtcgggtcg gccatgccga ctcgataaaa cgtcgctgcg
      781 tcctccagta cgatggctcg aaatcgccgc gccaatgttt caggaagaag gtttgcaaca
      841 tcaggacgga tattgaaacg tttcaggtcg acgtaaggaa cctgcagctg gcgtgcaagt
      901 gcgcttccga gttgctcttc ggtaacgtat ccagcgtcga caaagatgcg cccgagcttt
      961 cgcccactgc gcttctgctc atcgagcgcg gattgcaatt tgtccgctgt cagcagttgc
     1021 tgctgaagca gaatctcgcc caggcgaatt ttctcgggac gtcccatgaa ggctaccggg
     1081 ccagctcttt ccgcataact ttaggtaaat gttgcagctt attgaattgc aagattaaac
     1141 cttaaatcat ttgaattgca actaaattgt tcgatatcgt cctgtttgaa ccggaaattc
     1201 ctcccaacac gggtaatgtg atccggcttt gcgccaacac gggttgcagg ctgcacttaa
     1261 tcgggccgtt gggatttagg ctggatgatc gtcaactcaa aagagcgggg ctggattacc
     1321 acgagtacgc tagtgttcgg gttcacgcca attggttgga tgcgctgaag gccattcctg
     1381 gccgcaaatt tgccttcagc accaaaggga taaatcgttt cgacagcgtg gactttcgtc
     1441 cgggagacgc ttttgttttt ggccctgaga cccgcgggtt gccggccgaa ttgttgcaga
     1501 gcttcgatcc atcgtttcgg cttcggctac ccatggtggc gcacagccga agcctaaatc
     1561 tttccaacgc cgttgctgtc gtggtctacg aagcgtggag gcagaacggt ttctcaggta
     1621 tggccagtta gcgcacgctt tcttgcttgg gcccgcgttg caacagttcg ctaactaaat
     1681 ctcgtgcgga agtcgagccg ttcagcaatt cgaaaacggc cgccgttatg ggcatgtcaa
     1741 tcttcagttg ggctgccaga tgcagaacct cgctggcgct gtgaactcct tcggctacgt
     1801 gcccgagttg agcaaggatc gctgacaaac cttcgccgtt tgccagctga atacctacgc
     1861 ggcgatttct tgataaatcg gaagttgctg tcagcaccag atcgccggca cccgcaagac
     1921 ccatgaaagt gtcggagcgg cctcccaatc gcatccccaa tctggtgatc tctgcaagac
     1981 cgcgggtgat gaccgctgca cgtgctgacg cacccaggtg caagccatcg caaacgccgg
     2041 cagcaatcgc aatgacgttt tttacggcgc cccccgtttc gacgccgatg acatcatcgc
     2101 ttgaatagac ccggaatacg ccggtatgta atgcctgtga tgccagcgcg gcgaatttgt
     2161 tatccgagga tgccagcgtg acggccgcag gcaaaccccg cgcgacttca ctggcaaagc
     2221 tgggacccga aagaaccgcg cgccgccgcg cgggagacag ttcttcttcc gcaacttgat
     2281 gcgcgaactt tgccgtgccc ggttcaaagc ccttgcacaa ccaaaccaca cccgccgcag
     2341 gtgagctcgc cgcgattagt ttgagggttt gtctcaaggc cgccgtagga acggcaacca
     2401 caatcatgtc gccatgttgc agtgccaggt tcatatcagg cgtaatccga acgtgctgcg
     2461 gaatcaccac accaggcaaa tagagcgcgt tgatacgttc tgtcacgaga ctacggcatt
     2521 gcgccggatc acgtgcccat aacaaaatct catgatgtgg ggaaagtgcg atgcccagcg
     2581 cggtacccca ggcgccggca cccagaatgg acagcctcat acgccccaga gctgacttac
     2641 tttgacgaat ccgatggcgc catcagcatg acggaccttg gcccagccgg tcgccatgcc
     2701 aatcaattcc aggaagacac ccttttcggc agtaaaaacg accggagaat cctcattggg
     2761 attttggcgc gcctctgcac ccgcgacctt tatcatcaca gtatgccgat ccgacaatcg
     2821 cacggtttca atccaggcaa attcaccggt gtcgtcccgt accttggtcc agccctccaa
     2881 cacaacgacg acttcaaccg gatagccgcg cgaaagaaga aaaagttttt gtgcttttgt
     2941 ggacggcgca tcgtacagaa ttgctgcgga ttcgctgacc gagcgatatt ccagagctga
     3001 cacattggcg ctcaccagca ggcccatcag cgccaccaac cgcagcttca cgatcatcgt
     3061 ggaatcaatg cgtcgtcgca ccggctgctg ctgactgcac ccgggcgtca cgctgcgctt
     3121 gaaaaattgc ctcgaaatta acgggattca gtaccaccgg cgcaaacccc gctcgcacta
     3181 cggcgtctga tatgacttcg cgcacgtatg gatacaagat gttcgggcag gtgacgccca
     3241 acacgggttc cagttcggtc tgtggcacat ggcgtatttg aaatacgccg ccctggcttg
     3301 cctccaccag aaaaacagtc ttctctgcca gtttagcggt cacggtcacg ctaacgacga
     3361 cttcgaaata gccttcgtcg atcggattgg cggcgctggt catttggatt tcaatttgcg
     3421 gcgtatcccg ttccagataa attttcggtg catttggcac ctccaccgag agatctttta
     3481 cgaacagctt ttcgatcgaa aacacgggct ggttaggttg ttggctcatg gtggctctgg
     3541 gctgttcgga tgatgaaaag gggctcagct acgggtcggt tcgggcgcct ggtgcaacaa
     3601 ggggtccagc tcaccggctg cgtccagcgc cgccaggtcc tcaaaaccgc cgacatgtcg
     3661 atcatcaata tagatctgtg gaacggttct tcggccggtg cgttccatca tttccgcgcg
     3721 ctttgcaggc tgcgagtcaa tcctgatctt ggtgatgtcc gatacccctt tactctttaa
     3781 cagcctctcc gcagcgatgc aataagggca taccgccgtg caatacatgc tgatgtgcgg
     3841 catcatttct ccaccggcaa attggcctgc tgccatgcgc cgatgcctcc cgccaatacc
     3901 accacctcgg caaaacctgc cttcttcaac tgcgtcgtcg cagcatggga ccggttcccg
     3961 gtctggcaaa cgagaacggc aggccggttc ttgaactttt ccagctcacc gatgcgcttg
     4021 tcgagttgtg gtaaaggaat atttctagag ttcggtgcat ggcccgcgct gaactccgcc
     4081 ggctcccgta cgtcgatcat tacggcattc ttgcgattga gcaactgtac ggcttgcatt
     4141 gcgccgattt ccggcacacc actcaaccgc cgattgataa ggggccaaat cagaatagcg
     4201 ccgctgatta atgcggccaa caccagccag atattgttca gcacaaattt cactttttag
     4261 ccttttcgtt ttgttgctgc gcatagctca gtaaatcttt cattccagtc tggcccgatt
     4321 tttccagcgc ttgcgccacc ttcaaagcct cttcccggtc actgggataa ttagcaaccg
     4381 ccagatccca ttgtgcgcgc atcccggcat ggtcacccga taacgctagc aacagcgcct
     4441 ggcgataggc aaaatcattg gaaggctgaa aacgcatcgc acgtgcattc agcacgactt
     4501 tatcctgaag gagttcctgg ttaagcaaca tgcgccgggc caacgcgaac tcgatgaacg
     4561 gagcaaacag ggaggcacgt tgcatatcga gcagcacgcg aatgctaacg tcgtcggatc
     4621 ccgtggccga ttcagctggg accgtaccct gtagcgactg catggcgcga tagtcaaggc
     4681 acacattcgt gaatgcaaat gcacccagca caacagccgc acacaggact agcctgccac
     4741 ttgacccttg aactatcgca ggcgcatcac gttccgccgc gcccatgatc atcgcaaaaa
     4801 gccccagaaa atacgcatac cacaggggat actctagcat actgtggatc ccgagcactg
     4861 cgatggccgc cagcatccac catagcggag aatcgatttg ggcgcgtcgt aatgaagctc
     4921 cccaccaacc caggccgctc aacagcaaga ttaatcccgc agcaccgaat tccgcggca
//
LOCUS       DATMZQ010000059         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3149337, whole genome shotgun sequence.
ACCESSION   DATMZQ010000059 DATMZQ010000000
VERSION     DATMZQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149337"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN496_02040"
     CDS             complement(<1..381)
                     /locus_tag="VN496_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15750.1"
                     /translation="MFRGHHRKVRLLFGVSDILLTALAFLLAYRVRVLLDHVPLEHDF
                     HLDSPVLVLLLGGSMIMWVAIGTWWQIYDRIDAAHPRIILRDTFRQCLMGAVAIVLFE
                     FALRLDLSRSFLAMFAAFNWALLCV"
     gene            552..2033
                     /locus_tag="VN496_02045"
     CDS             552..2033
                     /locus_tag="VN496_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXF15751.1"
                     /translation="MSCLQRFIPARLPAVKIRNAAVSLGVSAILLTPLTFTHAQQGKP
                     MVPNLAGFPDPSGVVRTYSENGDIDLTGPFFQSLGTNGRSCGTCHQPSDAMSVAAAHV
                     QTRFDQSQGLDPIFRTNDGSNCDHNIDVSTLAGRSAAYSLLRTRGVIRVALDVPATRD
                     FDIVSVSNPYGCGETNVISMYRRPLPAANLMFLSALMFDGRESSTQTGTLPITSVNYP
                     QSLLSDLAHQSLDATTGHAQGVGLTTAQQQAIVNFETGLYTAQAQDHGAGDLASGGGE
                     GAPQPISHQKYYIGINDPVGLDPENPVPLNFSTRIFSIYDAWQNAKNPQRQAIARGQA
                     IFNTKAFTITGVAGLNGFTFSNGVTGPTTIVSTCGICHDSPNAGNHSVSAALDIGVAD
                     PPGGNNVLDTSYLPVITICPKDAATPCVQTTDPGRAMITGKFADVGKFKGPILRGLAA
                     RAPYFHNGSAQTLQDIVNFYDARFHIGFTAREKSDLAAFLNTL"
BASE COUNT          465 a          617 c          572 g          395 t
ORIGIN      
        1 aacgcacagc agcgcccagt tgaaggcagc gaacatggcg aggaaagagc ggctcaaatc
       61 caagcgcaga gcaaactcaa agagcacgat ggcgacagct cccattaggc actgacggaa
      121 ggtgtcacgc aggatgatgc gcggatgggc ggcgtcaatg cggtcataaa tctgccacca
      181 ggtgccgatc gcgacccaca tgatcatcga tccgcccagc agcagcacga gcaccggcga
      241 gtctaaatga aaatcgtgct cgaggggaac gtggtccagc agaacgcgca cccggtaagc
      301 cagaagaaag gcaagcgcgg ttaagagaat atcggagacg ccgaacagca gccggacttt
      361 gcgatggtga cctcgaaaca cgtgcgatgc cagaatacca ccaaagaggg gacagacgat
      421 atttccgtat ttcccggaaa tattttccgt cggcggctat cccttttctt gcaaccaggt
      481 actaagcgaa cttgatactt gcgccacgca ggagttcggg gtagtatatt tccaatctat
      541 cggaggaaag catgagttgt ctgcaaagat ttatccccgc ccgtctgcct gcggtcaaga
      601 ttcgaaatgc ggcggtctcc ctcggtgtct cggcaatttt actgactccg ctgaccttca
      661 cgcatgcgca gcaaggcaaa ccgatggtgc ctaacctggc cggctttccg gaccccagcg
      721 gcgtggttcg gacttacagc gagaatggcg acattgacct caccggcccg ttcttccaaa
      781 gtctcggcac caatggccgc agctgcggga cctgccatca gccgagcgac gccatgagtg
      841 ttgccgccgc gcatgtgcag acgcgcttcg accagtcgca aggactcgat ccaatttttc
      901 gcaccaacga cggctcgaat tgcgatcaca acatcgatgt ttcgacgctg gcgggccgct
      961 ctgccgcgta tagtctgctg cggaccagag gagtgattcg cgtcgcgctc gacgtgccgg
     1021 caacccgcga tttcgatatt gtcagcgtca gtaatccgta tggttgcggt gaaaccaatg
     1081 tcatttcaat gtaccgacgt ccgctaccgg ctgccaacct aatgtttttg agcgcactga
     1141 tgttcgacgg gcgcgaatcg tcgacgcaaa ccgggaccct gccgatcacg tcagtcaact
     1201 atccgcagtc gctgttgagc gatctggcgc atcagtcgtt ggatgccacc accggccatg
     1261 cgcagggcgt tggactgacg accgcccagc agcaagcgat cgtaaatttc gaaaccggat
     1321 tgtacacggc gcaagctcag gaccacgggg ctggagactt ggcctctggg ggcggcgagg
     1381 gagcgccgca acctatcagc catcaaaagt attacatcgg gatcaacgat ccggtgggat
     1441 tagacccgga aaaccccgtc cccttgaatt tcagcaccag gatttttagt atttacgatg
     1501 catggcaaaa tgcgaaaaac ccgcaacggc aggccatcgc gcgtggacag gcgatcttca
     1561 acaccaaagc ctttacgatt accggggtcg cgggcctgaa cgggtttacg ttctccaatg
     1621 gcgtcaccgg gcccacaacc atcgtctcca cttgcgggat ctgccacgac tccccgaatg
     1681 ccggcaacca ttccgtgtcc gctgctctcg atattggagt ggccgacccg cctggcggca
     1741 acaatgtgct ggacacgagc tatcttcccg tgatcacgat ctgcccaaag gacgctgcca
     1801 cgccctgcgt gcaaactacc gaccccggac gcgccatgat caccggcaag tttgccgatg
     1861 tcggcaagtt caagggaccg atcctgcgtg gcttggccgc tcgcgcgccc tatttccaca
     1921 acggcagcgc gcaaacgctc caggatatcg tgaacttcta cgacgctcgc ttccatattg
     1981 gcttcacggc tcgggagaag tcggacctgg cggcgttcct caatacgctg tgaccggttt
     2041 ctcctgaat
//
LOCUS       DATMZQ010000060         8389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2461029, whole genome shotgun sequence.
ACCESSION   DATMZQ010000060 DATMZQ010000000
VERSION     DATMZQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461029"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="lptC"
                     /locus_tag="VN496_02050"
     CDS             complement(<1..470)
                     /gene="lptC"
                     /locus_tag="VN496_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXF15752.1"
                     /translation="MNDRLITWSPLILLALLASLSFWLDRKVQPQAHLPDGSTRHDPD
                     FIIEGFSAVKMNPDGTRRYALAAKRMVHYPDDNSTQLELPKLVYFDYQRAPVTIRSET
                     AEVAQGGDDVFFRGDVQIIRSAYERNAELGVFTSFLHVIPDQDMAKTDKPVRMV"
     gene            complement(467..994)
                     /locus_tag="VN496_02055"
     CDS             complement(467..994)
                     /locus_tag="VN496_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HXF15753.1"
                     /translation="MNKRLSARANRIRLAIFDVDGVLTDGTLYLSERGEVLKAFSILD
                     GVGLKLLSASGVATALLSGRKSQTVAVRAKELGIPHVLQGIDDKLEAYHRLLRKLGFA
                     EEQTSFMGDDLPDLPVLRRCGLAFSVPNAPDIVRSHAHFVTNASGGHGAVREACEFLM
                     LARGTLESQLRTYLA"
     gene            complement(1040..1993)
                     /locus_tag="VN496_02060"
     CDS             complement(1040..1993)
                     /locus_tag="VN496_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXF15754.1"
                     /translation="MLESGRRVLEVEARAVLGLASRLDVNFVRAAEIVLACRGRVVVS
                     GIGKSGHIARKIASTFASTGTPAFFVHPAEASHGDLGMVTRDDVMIALSNSGESGELL
                     LIVPLIKRMGGKLIAMTGNADSPLAVEADVHLDAGVDQEACSLGLAPTASTTAALALG
                     DALAVTLLEARGFRAEDFARSHPGGSLGRRLLTHVRDVMRSGDAIPRVPDTATFTEAV
                     LEISRKGMGMTAITSNNRVVGIFTDGDLRRALETGDDLRDLGVKKLMKPTPRCVGPDQ
                     LAAEAADQMEQYRITQLLVTDNDQNLIGALNMHDLMRAKVI"
     gene            complement(2209..2886)
                     /locus_tag="VN496_02065"
     CDS             complement(2209..2886)
                     /locus_tag="VN496_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXF15755.1"
                     /translation="MTNRTKNRGFTLIEIAIVLVIIGLLLGGVLKGQELINNLRVKSF
                     ANDFRNIPVYIYSYQDRFRAIPGDDQQANTHVAGVNATTPAGTLNNGQINGAWNSATN
                     TDESCLFWSHIRLANLAPGATTAACGAAGYYPVNASGGQIGVQSSNGFTTITLPTAMT
                     GTYTICSANISGQFVLQLDTLLDDGNPQTGSMRAIDQTALAAQTAATTAAVQANPGGN
                     YTVCLSV"
     gene            3283..4992
                     /locus_tag="VN496_02070"
     CDS             3283..4992
                     /locus_tag="VN496_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF15756.1"
                     /translation="MNAETTAENSIRHAVAAIRQQHWLSLALLALHCALMLELSDPLS
                     RALLLSHFGLFLLWQPLWRGGQKLFPGQVGLIIGAAVLLIASSSYWMMALWISVLFSL
                     IGGNVPGIKNVRQRIVSLLAAIYLLSVLLMWVVPHIFSDQLFSGPFLVLARYGLVIPA
                     LAIFFVRTEKSQSSSAYSVDLFYSLLLFLMVVVLVLGAFAIKQVSHGNYVMSLAQALI
                     VAAGLLVALSLLWDPRAGFAGIGQLVTRYFLSIGVPFERWMHSLAGLADRERDPDKFV
                     IVAAHEMAGLPWLSGIGWQSKTSTGSAGETTKYATEFVFGGLKLRLYTQWSPSPALVL
                     HIRLLARLLGDYYDAKMREQDQRRNAYVQAIYETGSRLTHDVKNLLQTLRSLCAAAET
                     SQEDDSDALRLLMQRQLPQITQRLQITLDKLNTKPALAHDKIPGTQWWNELQQRFAHE
                     RVEFVGQMLPQGSMLPTDLFTSAAENFLQNAIEKRKLNTALRILVQLQWIDGFVLSVC
                     DDGQPVSESLTGELFNSPVQSKSGLGVGMYQVARFAKEQGYEVSLSSNQPGRVCFALS
                     PMR"
     gene            complement(5013..5780)
                     /locus_tag="VN496_02075"
     CDS             complement(5013..5780)
                     /locus_tag="VN496_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXF15757.1"
                     /translation="MMHKNSGFSLIELAITVFIIALILGSLLVPLQSQIRSRKLDETD
                     RILNQAREALLGYVSANGYFPCPASGASNGQEASPNHGTGTCNASVTFATALVGFLPS
                     VTLQLNPVDGSGYLLDAWGLPQSRIRYAVANATVNGITNPFTRTNGMRNAAMANIQGA
                     TTLLFVCRTGTGVTATGCAAATDELSDNAIAVIWSLGENAPTGGGAPHEDKNLNNTRV
                     FVQAPYSTTAGSIFDDQLTWIGPPMLFNRLIAAGQLP"
     gene            complement(5785..6939)
                     /locus_tag="VN496_02080"
     CDS             complement(5785..6939)
                     /locus_tag="VN496_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15758.1"
                     /translation="MLVVIVLGAGFLVYSAAGKHSAKMDRQNKTDAALAIARDALLGR
                     ALSDSNRPGSLPCPDVNDDGQLTLGVDFGAGGACMQTIGRLPWRTLELPELRDGDGEH
                     LWYAISPAFSDTTTNPINTDSIGSITVYSGSTTSVVSSRAVGVVFAPGAPLGNQARSP
                     TQTALCPTTGTTIAQSLCANNYLDLDTASGINNANATGPYITVATSGTFNDRMLVVSD
                     SVDLMTPIEARAASAILTALQAYKAGSGTWWGCNCYPWADIGDGFANDALEHGRVPLK
                     GADTGHSNDWADVGVTIPQWLIDNQWWLVFLYTVAPNRTYDHNFGSLTVNGVAGTADI
                     VLISAGAAISARATFPTDYIEDAENNDFGVVFITPTDTASKNRDRLYTLP"
     gene            complement(7568..8155)
                     /locus_tag="VN496_02085"
     CDS             complement(7568..8155)
                     /locus_tag="VN496_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15759.1"
                     /translation="MGAFKTPAIAVTVAIAVSLAMILFSSNQHVQSEKQYGNQLAALK
                     EAKNRYQRSGEERETVMHYILAYQQLEKIGFIGAERRLNWVEDLREANAQAGLFGVDY
                     QMMAQEPYTYVAKDNPIGDRIKQSKMRLSFGLLHEADLMKLFRALADRQPGLFSITEC
                     SLDRAGREGVPSPRQANLAAQCELSWVTIVRKEKS"
     gene            complement(8177..>8389)
                     /locus_tag="VN496_02090"
     CDS             complement(8177..>8389)
                     /locus_tag="VN496_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15760.1"
                     /translation="TRFRGDYRAAMSIINHFVQQIAREDAVSDVNILKLPLDVRSEAA
                     LTGSTAAAAGKEKAQFEIQIVFRSGA"
BASE COUNT         1790 a         2318 c         2313 g         1968 t
ORIGIN      
        1 tccaccatac gcaccggttt gtccgtcttt gccatgtcct ggtcaggtat gacgtgcagg
       61 aaactggtga acactcccag ctcggcattg cgctcgtaag cggagcggat aatctgcaca
      121 tccccacgga agaatacgtc atcaccacct tgcgcgactt ccgcagtttc ggaacgaatg
      181 gttaccggag cacgttggta atcaaagtaa acgagcttgg gtaattcaag ttgagtgcta
      241 ttgtcatccg gataatgcac catccgcttc gctgccagtg cataacgacg cgtcccgtcg
      301 gggttcattt tgaccgccga aaagccttcg ataatgaaat cgggatcgtg gcgcgtgctg
      361 ccgtctggca aatgagcctg cggctgcacc ttgcgatcga gccagaaact taacgacgcc
      421 agcagcgcca ataaaatcag aggtgaccac gtgatgaggc gatcattcac gctaggtaag
      481 tccgcaactg actttccaaa gttccccgtg ccagcatcag aaattcgcag gcttcgcgca
      541 ccgcgccgtg cccccccgaa gcatttgtga cgaagtgagc atggctgcga acgatgtccg
      601 gtgcgtttgg gacgctgaac gcaagcccgc accggcgcag cacgggaaga tcgggcagat
      661 cgtcgcccat gaaagatgtc tgctcctcgg caaatccaag cttgcgcaac agccggtgat
      721 aagcctccaa tttgtcatca atgccttgca atacgtgcgg tattccgagc tccttggcgc
      781 gcactgctac tgtttgggat ttccgtccgg aaagcaatgc ggtggccacg ccactggccg
      841 ataacaattt caggccgact ccatcaagaa tgctgaaggc cttgagtact tcgccgcgtt
      901 cgctcaaata gagcgtgcca tcggtcaaga ccccgtcgac gtcaaagatt gcgagccgaa
      961 tacgatttgc tcgggcactc aatcttttgt tcatctcacc accgctggaa tcttgcgagc
     1021 tgctcgatgt ctcgcatgac taaatcactt ttgcccgcat gagatcgtgc atattcaatg
     1081 cgccgatcag attttggtcg ttatccgtga ccagcaactg cgtaattcgg tattgctcca
     1141 tctgatcggc tgcttcagcg gcgagttggt cagggccgac gcagcgcgga gtgggcttca
     1201 tcaatttttt cacaccgaga tcgcgcaaat cgtcgccagt ttcaagtgct cgacgcaagt
     1261 ccccatcagt gaaaatgcca acgacgcgat tgttactggt gatagcggtc atgcccattc
     1321 ctttcctcga aatttcgaga accgcttcgg taaacgtcgc agtatccggc acacgcggaa
     1381 tagcatcccc tgagcgcata acatcgcgca catgcgtcaa cagccggcga ccaagactgc
     1441 cgccgggatg cgagcgcgca aaatcttccg cgcggaatcc gcgtgcttcc agcagcgtaa
     1501 ctgccagcgc gtctcccaat gcaagcgcgg ctgtcgtgct agccgtaggc gcgagtccaa
     1561 gggaacaggc ttcctggtca acgccggcat cgagatgcac gtctgcttca actgcaagag
     1621 gcgagtccgc attgccggtc atagcaatga gcttcccgcc catgcgtttg atcagcggca
     1681 cgatcagcaa caattcacca ctttctccgg aatttgaaag cgcaatcatg acgtcgtcgc
     1741 gagtgaccat gcccagatcg ccgtgactcg cctcggccgg atgcacaaaa aatgctggtg
     1801 taccggtact cgcaaacgtg gacgcgatct tgcgcgcaat atggcctgat ttgccaattc
     1861 cactgaccac gacccgcccc ctgcaagcca acacgatctc tgcggcgcga acgaaattca
     1921 catcgagtcg gctggccagt ccgagtaccg cacgcgcttc cacttccagc acgcgccgcc
     1981 cagactccag catttgccgc gcatccagtt ttccgagtcg tgtcatgggg ttcgagtata
     2041 acgcatggtc cccgtcattt ttcgcggaaa caatcgtgta gcacttgcat taatcgctct
     2101 cgtgcacgca catgtttggc cgaatgcccc ggggatcgtg gtgagctgaa agctgccaaa
     2161 aaaaagcccg cgagacgcgg gctatcgggt cctgcgatgg gcgatgtact acacgcttag
     2221 acagaccgtg tagttgcccc cgggattggc ctgtacggcc gccgtcgtgg cggcggtctg
     2281 ggctgcaagc gcggtttggt caattgcgcg catcgaaccg gtctgcggat tgccatcgtc
     2341 aagcagtgtg tcaagctgga gaacaaactg accggagata ttggctgagc agatggtgta
     2401 cgtgccagtc atggcagtcg gtaatgtaat tgtcgtaaag ccgtttgagc tttgcacacc
     2461 gatctgcccg ccggacgcgt tcaccgggta gtaacccgct gcaccgcaag cagcggtcgt
     2521 cgcgcctggg gcaagattcg ccagccggat atgcgaccag aacagacagc tttcatcggt
     2581 atttgtggct gaattccacg cgccattgat ctggccgtta ttcaaagtcc cagccggtgt
     2641 ggtggcattg acgccggcaa cgtgggtgtt cgcctgttga tcgtcacctg gaatcgcacg
     2701 gaagcggtct tgataagaat aaatataaac cgggatgttg cgaaaatcat tcgcgaaact
     2761 cttcacccgg aggttgttga ttagttcctg gcctttgagc actcctccca acaggagacc
     2821 aatgatcacc agcacgatgg cgatttcgat cagtgtaaat cctcgatttt ttgtccgatt
     2881 cgtcatgatg actccatttc ccgggaaaat gcccagtgtc tcgccttacg ccagaactgt
     2941 gcccaagctt tggaataccc gctgcccaaa ttatccggga gtgctatgac ggggccataa
     3001 caggaaatga agagcaaggt attgaaagtt aaacggtatg cacaggggga cgcgaacacg
     3061 cgacgcttgt ggagcttgat ttttttccga cccgaaatcc cattaaaccg ttatattttc
     3121 gtcgaatccg acgagttttc gtcaaaccgg gccccgcacg caatttcaat caagcgtctg
     3181 agctttttta gtcgcgggac aggaaaaagc atattcaggg ttattccaac tccgaatatt
     3241 agagccgctg cggtacacta tttccatggc gattcctccg aaatgaacgc ggaaacgacg
     3301 gcagaaaatt cgatacgcca tgccgtggcc gcaatccgac agcagcactg gctgtcgctg
     3361 gctctgctgg ccttgcattg cgccttgatg ctggagctgt ctgatcccct gtcgcgcgcg
     3421 ctgttgttgt cgcactttgg attgtttttg ttgtggcagc cgctttggcg tggcggacag
     3481 aaactttttc cgggacaagt cgggctgatt atcggcgctg ccgttctcct gatcgcttcc
     3541 tcgagctact ggatgatggc gctatggata tcggtactgt tttccctgat agggggtaac
     3601 gtaccaggga tcaagaatgt ccggcaaaga attgtttcgc tactcgccgc gatttatttg
     3661 ctatcggtgt tgctgatgtg ggtagtaccg cacatctttt ccgaccagct tttttccggg
     3721 ccttttttgg tattagctcg ctacggacta gtcatcccgg cactagcaat cttctttgtg
     3781 cgaactgaaa aatcacagtc atcgtcggcc tattccgtag acctttttta cagtctgctg
     3841 ctctttttga tggttgtcgt attggtgctg ggtgcatttg caatcaagca agtcagccat
     3901 ggaaactacg tgatgtcgct ggcgcaggcg ttgatcgtcg cggcgggcct gctggtggca
     3961 ttgtcgctgc tatgggaccc tagggctggc ttcgcaggaa tcggtcaact tgttacccgc
     4021 tatttcttga gtatcggcgt gccgtttgag cgctggatgc atagcctcgc ggggttggcg
     4081 gatcgtgaac gagatcctga caaattcgtc atcgtggccg cgcatgagat ggctggattg
     4141 ccgtggcttt cgggaattgg atggcagtca aagacctcca ctggcagtgc aggggaaaca
     4201 acgaaatacg cgacggagtt tgtcttcggt ggcttaaagc ttcgtctcta tacgcaatgg
     4261 tcaccgagcc cggcgctggt gctgcacatt cgcttgttgg caagattgct gggcgactat
     4321 tacgacgcga aaatgcgcga gcaggatcag cgtcgtaatg catatgtgca agcaatatat
     4381 gaaacgggct cccgccttac gcacgacgtg aagaatctgc tgcagacctt gcgatcgttg
     4441 tgcgcagctg cggaaaccag tcaggaggac gactcggacg ctttacggtt gctgatgcag
     4501 cgtcaactgc cgcagatcac gcaacgcttg caaataacgc tggacaagct caataccaag
     4561 ccggctcttg ctcacgacaa gattcccggg actcagtggt ggaacgaatt acagcagcgc
     4621 tttgcgcacg agagagtcga gtttgtcgga cagatgctgc ctcagggcag tatgttgccg
     4681 accgatctat ttacctctgc ggccgaaaat ttcttgcaga acgcgatcga aaaacgaaag
     4741 ctgaatacgg ccttacgcat actggtgcaa ttgcaatgga tcgacggttt tgtactatcc
     4801 gtctgcgatg atgggcaacc ggtttccgaa tctttgacgg gagaattgtt caactcgccg
     4861 gtccaatcca agagcggtct tggagttgga atgtaccagg tggcgagatt tgccaaggaa
     4921 cagggctacg aggtttcttt gtccagcaat cagccaggac gggtgtgttt tgcactctct
     4981 ccgatgcgtt gatcttgtct gcgcactgcc ggtcagggca attggccagc cgcaataagc
     5041 cggttgaata gcatgggagg cccgatccag gtgagctgat catcgaagat cgaccctgcc
     5101 gtcgttgaat aaggtgcctg cacgaatacg cgggtattgt tgagattctt atcttcgtgc
     5161 ggtgcgcctc cccccgtcgg cgcattttcc ccgagcgacc agattaccgc gatggcgttg
     5221 tcgctcaatt catccgtggc ggctgcgcat ccggttgccg tcactcccgt cccggtacgg
     5281 cacacgaaca gcaatgtggt tgccccttgg atgttagcca tggccgcgtt gcgcatacca
     5341 ttggtcctgg tgaagggatt ggttatcccg ttaaccgttg cattggctac cgcatagcga
     5401 atgcggcttt gcggcaatcc ccatgcatca agcaaatagc cgctgccgtc gaccgggttc
     5461 aattgcagtg tcaccgacgg caagaaacca acgagtgctg tcgcaaaagt cactgaggca
     5521 ttgcatgtac cggtgccgtg attgggagat gcttcctgac cattgctggc gccgcttgcg
     5581 gggcacggaa agtagccgtt ggccgagaca taaccaagca gcgcttcgcg ggcctggttg
     5641 aggattcgat cggtctcgtc caacttacgg ctcctgatct ggctttgcag aggaacgaga
     5701 aggctgccaa gaatgagcgc tatgatgaag accgtgattg ccagttcaat caagctgaag
     5761 ccggagtttt tgtgcatcat gtgttcaagg tagcgtgtac agtcgatccc tgttcttact
     5821 ggccgtgtcg gtcggggtaa tgaaaacaac tccgaagtcg ttgttctcgg cgtcctcgat
     5881 gtaatcggtc ggaaatgtgg cccgggcgct tattgcggca cccgccgaga tcagcactat
     5941 gtcggcagtc cctgccacgc cgtttactgt cagggaaccg aaattgtggt catacgttct
     6001 attgggtgcg acggtataca aaaataccag ccaccattga ttgtcgatca accactgcgg
     6061 aatagtgacg cccacgtcgg cccagtcgtt gctgtggccg gtatcggcgc ctttcaacgg
     6121 aacgcgacca tgttcgagcg cgtcattggc aaacccgtct ccgatgtcag cccaggggta
     6181 gcagttgcag ccccaccagg ttccgcttcc agctttgtac gcctgcagcg cggtgaggat
     6241 tgcgctggcg gcgcgcgctt caatcggcgt catcaaatcg acggaatcac ttactaccag
     6301 catacggtca ttgaaggtcc cactggttgc aacggtgatg taggggccgg tcgcattcgc
     6361 gttattgata cccgatgcgg tgtctagatc cagatagttg ttcgcacaca aagattgcgc
     6421 aatcgtagtg ccggtggtcg gacaaagtgc ggtttgcgtc gggctacgag cctggttacc
     6481 gagaggagcg cccggagcaa aaacgacgcc aaccgcacga ctcgagacca cgctcgtcgt
     6541 gctacctgaa taaaccgtaa tgctgccaat cgaatccgta ttgatcggat ttgtggtggt
     6601 atccgaaaag gccggagaga tcgcgtacca taggtgctct ccatctccgt cgcgcaattc
     6661 tggcaactcc agcgtccgcc acggcaatct tccaatggtc tgcatgcagg cgccgccggc
     6721 gccgaaatcg acgcctagcg ttagctgacc gtcatcgttg acgtcgggac aaggcagcga
     6781 accgggccta ttggaatcgg atagtgcgcg gcccagcaag gcgtcccgtg cgatggccag
     6841 tgcagcatct gtcttgttct gcctatccat ttttgctgaa tgtttgccgg ctgcgctgta
     6901 gacgagaaat ccagctccca ggacgattac tacgagcatc agaattatcg tttgtccacg
     6961 ctgcaggacc gcaggagctg cacgcaagcc tcgttgtctg ttcgtggaca atgttgcgcc
     7021 gtcgtcccaa aaatctatcg gcttacaggc gcggaaggag ttcggccctt gtcatccgcc
     7081 ggggagcctg gacctttcac cattgcggac tccttggttt gcatcggagg caccgtcggg
     7141 gcctctacgg ggacgctttc cgccgagacc ctttgggaag gcttatctgc gactccgccc
     7201 gtattcagat ccagctgctg tccaactttg attcgagcgg cgtgttccct gccccttacc
     7261 cgtatttccg tcgtctcagg cgtagatgga ttggtcctta cctgaagccc ttcgggagac
     7321 ccgtcgtggt aggctttacc gttgatccac acggtgcgct cagcatcact gcgggtgacc
     7381 acgccattta caacgacgtt tcgaggcgca gttaccggtg ttgacgcttc gcctaacgat
     7441 ttgcctgcat cgagcacgct gcgctgtgcc ggagtgaaga aaagcctgcc gagcggttcc
     7501 gccgcggatg tcggtgccgt ccaagccgcc aaggtcgcga ggcatagtat ccaaaacgtc
     7561 gcgatgctca agatttttcc ttgcgaacta tcgttaccca ggacagctcg cattgggccg
     7621 ccagattggc ttgcctggga gatggaacgc cttctcgtcc ggcgcgatcg agcgagcatt
     7681 cggtgattga aaacaaaccg ggctggcggt cagccagcgc gcggaacaat ttcatcagat
     7741 ctgcctcatg cagcagcccg aaagacaatc gcattttcga ttgcttaatg cgatcaccga
     7801 ttgggttgtc cttcgcgaca tacgtatacg gttcttgtgc catcatttga taatccaccc
     7861 cgaacaagcc ggcttgcgca ttcgcctcac gtaaatcttc cacccaattg agccgtcgct
     7921 ctgcgccaat aaaaccaatt ttttccagct gttgataagc cagaatgtaa tgcatcacag
     7981 tttcacgctc ctcacctgag cgttgatagc gatttttcgc ttccttaagg gcagctagct
     8041 ggttgccata ctgcttctcg gattgaacat gttgatttga gctgaacaat atcattgcaa
     8101 gagatactgc aatggcaacc gtgaccgcaa ttgccggtgt tttgaatgca cccaagtcct
     8161 ttgatgtcag gttcatctaa gccccagacc tgaacacaat ttgaatttcg aactgggcct
     8221 tttctttccc ggcagcggcc gcggtactgc cagttaaggc cgcctcggat cgtacgtcga
     8281 gtggcaattt caatatattc acatcggaga cggcatcttc tcgcgctatt tgctggacga
     8341 aatgattgat gatgctcatg gctgcgcgat aatcgcctcg aaaacgggt
//
LOCUS       DATMZQ010000061         3097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_713554, whole genome shotgun sequence.
ACCESSION   DATMZQ010000061 DATMZQ010000000
VERSION     DATMZQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713554"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1355
                     /gene="serS"
                     /locus_tag="VN496_02095"
     CDS             69..1355
                     /gene="serS"
                     /locus_tag="VN496_02095"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXF15761.1"
                     /translation="MLDVQLIRTDLDVVAQGLAARGVTIDVTAFRTLEEQRKKAQVRT
                     QELQSRRNASSKTIGELKRKGGDTSALMTEMSAVGDELKAIEGDLETIQAKLTSMLLT
                     VPNLPHPSVPVGKSAENNVELRRVGEPRKFDFPAKDHVDIGAGLGLLDFDAAAKISGA
                     RFTLMKGLLARLHRALAQLMLDIHTGEHGYTEVYVPYLVNADSMRGTGQLPKFEDDLF
                     AVPRAEGGKLYLIPTAEVPVTNIVRDEVVPGEKLPLKFVCHSPCFRSEAGSYGKDTRG
                     MIRQHQFDKVELVQIVQPDKSYEALEQLTGHAETILRRLELPFRTVVLCTGDMGFSAA
                     KTYDIEVWLPGQNAYREISSCSNFEAFQARRMQARFKTEKGKNELVHTLNGSGLAVGR
                     TLVAILENYQQADGSVLIPSALRPYMGGASRITAVG"
     gene            complement(1532..2968)
                     /gene="mltF"
                     /locus_tag="VN496_02100"
     CDS             complement(1532..2968)
                     /gene="mltF"
                     /locus_tag="VN496_02100"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HXF15762.1"
                     /translation="MKRRGVFPFERLRPLGTFAAALLLTLLSCNRDSSDLPVAPFGES
                     DELVVLVANGPATRFLGADGKYSGIEQDLLELFAKDEGLRLRLVERAKFSEILPALRH
                     HRAHLAAAGLAATEERRKHFLFGPAYLSVHTVVAYNTDNQRPKNVRDLLGKRVVALAG
                     SSAAEQFRLELLENPGLKWDEVAATDSMELLDRLAADEVDYVITDSNIVELAQNFSSN
                     IGRAFNLGKPETLSWAMPKDASPLLVERVDDFFTRINENGTLRVLLDRYYGHIQRLTQ
                     ADVSAFLERRLLVLPQYRSTFQEAQELTGIDWRLLAALGFQESHWNPLATSPTGVRGL
                     MMLTSETADRMGVTDRLDPHQNILAGARYLKILMDTLPDRITEPDRTWLALASYNVGY
                     GHLEDARVLTQRHGLNADLWVDVKKTLPLLTRSDFYITVKRGFARGGEAVVLTENIRN
                     YFSILLRYEAPYQPMFSAIHEEDGQLAP"
BASE COUNT          710 a          854 c          900 g          633 t
ORIGIN      
        1 agcgtagaga gcgtagtttc cgccttcgcg tatttcagaa aaaagccgaa agaatgcagg
       61 ggtaagcgat gttggacgtt caactgattc gtactgatct ggatgtggta gcgcaagggc
      121 tcgccgcgcg cggcgttacg atcgatgtta ccgctttccg cacgttggaa gagcagcgca
      181 agaaagcgca ggtacgcact caggaactgc aatccagacg caatgcttca tcaaaaacga
      241 ttggcgagtt gaagcgcaag ggcggggaca cctcagcgtt gatgactgaa atgagtgcgg
      301 tcggcgacga attgaaggcc atcgagggcg acctcgaaac catccaggcc aagctcacca
      361 gcatgctgtt gacggttccg aatcttccgc atcccagcgt gcccgtgggc aaatcggcag
      421 aaaacaacgt tgagctgcgc cgggttggtg aaccgcgcaa gttcgatttc ccggccaagg
      481 atcacgtcga tatcggtgcc ggacttgggt tgctcgattt cgatgcggcc gcaaaaatca
      541 gtggcgcgcg cttcaccctg atgaaagggc tgctcgcacg cctgcatcgc gccctcgcgc
      601 agctcatgct cgacattcat accggggagc atggctacac cgaagtgtac gtgccttatc
      661 tggtcaacgc agatagcatg cgcggcaccg gacaactgcc gaaattcgaa gatgatttat
      721 tcgccgtacc gagagcggaa ggcggcaagc tgtatctgat tcctactgcg gaagtaccgg
      781 tgacgaatat cgtacgcgac gaagtcgtgc ccggtgaaaa attgccgctg aaattcgttt
      841 gccattcgcc gtgcttccgc tcggaagcag gttcctacgg gaaagataca cgcggcatga
      901 ttcgccagca ccagttcgac aaggtggagt tggtgcagat cgttcagccg gacaaatcct
      961 atgaagcgct ggaacagttg accggccacg ccgaaactat tctgcggcga ctggaattgc
     1021 ctttccggac cgttgtgctt tgcaccggcg acatgggctt ttcggccgca aaaacgtatg
     1081 acatcgaagt ttggttgccc gggcagaatg cgtaccggga gatttcttcc tgcagcaact
     1141 tcgaagcatt ccaggcccgg cgcatgcagg cgcgcttcaa gacggaaaaa ggcaagaacg
     1201 aactggtgca tacgctcaat ggctctggcc tggccgtagg tagaaccctc gttgcgattc
     1261 tggaaaacta ccaacaggcc gacggaagcg tcttaattcc gtcggcgcta cgcccttaca
     1321 tgggcggagc aagccgtatc acggctgttg gttagtttca gagcaggcta acccaggcct
     1381 ttggggttaa gcgaagcggc cgaaactaaa actaccaaca ggccgacgga agcgtcttaa
     1441 ttccgtcggc gctccgccct tacatgggcg gagcaagccg tatcacgggt tggttagttt
     1501 cagagcaggc taacctgttg ctgcctggac actacggagc cagttggcca tcttcttcgt
     1561 gaattgccga aaacatcggc tggtacggcg cttcatagcg cagcaggatg gagaaataat
     1621 tgcggatatt ctcggtaagc acgactgctt cgccgccacg ggcaaatccg cgcttcacgg
     1681 tgatataaaa atcggagcgc gtcagcaggg gcagggtttt cttgacatcg acccacaggt
     1741 cggcgttcag cccatggcgc tgggtcaaca cgcgcgcgtc ttcgagatgg ccataaccca
     1801 cgttgtatga agcgagtgcc agccaggtcc gatcgggctc ggtaatccgg tcaggcaagg
     1861 tgtccatcaa gattttcaga tagcgggcgc cggcgaggat gttttggtgc gggtcaagcc
     1921 ggtccgttac gcccattcga tccgctgttt ccgacgtcag catcatcaat ccccgcacgc
     1981 cagtcgggct cgtggcgagg ggattccaat gcgattcctg aaagccgagc gcagcgagca
     2041 ggcgccagtc aattccggtc agttcctgtg cctcctggaa agtactgcga tactgaggca
     2101 ataccagcag gcggcgctca agaaatgcag agacatcggc ttgggtgaga cgctggatat
     2161 ggccgtaata gcgatccagc agtacccgca aggtgccgtt ttcattgatg cgggtgaaaa
     2221 aatcatcgac acgttccacc agcaatggac tggcatcttt cggcattgcc cacgacagcg
     2281 tttccggctt gccgagattg aatgcgcgcc cgatgtttga cgaaaaattt tgtgcaagtt
     2341 ccacgatgtt cgaatcggtt atcacataat cgacttcatc ggctgcgagc ctgtccaata
     2401 gttccatgct gtccgttgcg gcaacttcgt cccatttcaa tcccgggttt tccaggagtt
     2461 ccagccggaa ttgctccgct gcactggaac ccgccagtgc gacgacacgc ttgccgagca
     2521 ggtcgcggac gttcttcgga cgttgattgt cggtgttgta agccaccacc gtgtgcacgg
     2581 aaagataggc cggtccaaac aggaaatgct tgcgccgttc ttccgttgca gcgagacccg
     2641 cagcggccag atgtgcgcga tggtggcgca atgccggcaa gatttcactg aacttcgccc
     2701 gttctacaag acgcagtcgc aagccttcgt ccttcgcgaa cagttccagc aaatcctgct
     2761 ctatgccgct gtatttacca tccgcgccca gaaagcgcgt cgctgggccg ttggctacga
     2821 gcacgacaag ctcatccgat tcgccgaacg gcgccacagg caaatcggag gagtctctgt
     2881 tgcacgagag caatgtgaga agcagcgccg cggcaaaagt gccgagcggc cgtaatcttt
     2941 caaaggggaa tacgccgcgc cgtttcatgt ccaaggtccg aaactacgcg gctcaaatcc
     3001 gcaatgaact gcatcgctgg taaaataaca cacgaatcgg agaggtaccg aagtggccat
     3061 aacggcgctg actcgaaatc agatggtcag ggagacc
//
LOCUS       DATMZQ010000062         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3842881, whole genome shotgun sequence.
ACCESSION   DATMZQ010000062 DATMZQ010000000
VERSION     DATMZQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3842881"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1119
                     /locus_tag="VN496_02105"
     CDS             145..1119
                     /locus_tag="VN496_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF15763.1"
                     /translation="MNPPSPVIQLESVSKRYGSKNIIHDLSFSVGKGEIVGFLGPNGA
                     GKTTTMRMIAGFTTATSGRISVAGYDMATQNEEAARYIGYLPEQPPLYDVLEVSAYLR
                     FVARIKGVSARSIPAELERVIQACRLEAVITREIYKLSKGYRQRVGLAQALLGKPEVL
                     LLDEPTAGLDPGQIQETREVIRAFGENHAVLLSTHILPEVTLICQRVAIINHGRLLAI
                     DSPDGLQRASEQTNCVYLLATGPADAIRESILAIEGVSALDIKANAGRSELLSIECQV
                     DARDGVEAAIARAVAARWSLHRLERRQPTLENIFLRYIGETSSARASA"
     gene            1116..1823
                     /locus_tag="VN496_02110"
     CDS             1116..1823
                     /locus_tag="VN496_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXF15764.1"
                     /translation="MNGVFGIYQKELATYFRSPIAYFVIAVFLLGTGYFFIYNIFLTG
                     VGTMDETFQNMGILLLTLMPVMTMRLFSGEYSGRTMELLMTLPVRPWQIVTGKFLGAL
                     TMLLLMTFATAINLIPLYLYGTPESSTILSGYLGFLLLGMACLAVGQFFSALTQNQIV
                     AALITIPVLLGFWFIGHVQSFQTSPALRELFGYLSFALHFGDFIRGLVRTEAVMFYVV
                     VSAIALTLNTSYLKWRR"
     gene            1826..>2133
                     /locus_tag="VN496_02115"
     CDS             1826..>2133
                     /locus_tag="VN496_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15765.1"
                     /translation="MVRSFHSGLVLIAGIALLLGAAASHAIFLEIAAVTVILAVAGGL
                     LSAWGAYSLRSEIGGLLLHRRGEIVLYTLGIVGVLIALSYLSVRFPVRYDMTASKIYS
                     L"
BASE COUNT          423 a          605 c          586 g          519 t
ORIGIN      
        1 gaaggcgcgc aaatcttcat ggctcatgac ggtgatcagt tcaaagcgaa aggcgatcgc
       61 tggtacaaat aagcatgccg taattctttt ttgtcagaag ccccccctat gcgggggggt
      121 ttttttttaa accgtcgtct cattgtgaac cctccctccc cggtcattca gctcgaatct
      181 gtatccaagc gctacggttc gaagaacatc attcacgact tgtcgttcag cgtaggcaag
      241 ggcgagattg tgggatttct ggggcccaat ggagccggca agacaacgac gatgcgcatg
      301 atcgccgggt tcaccacggc gacttccggc cggatcagcg tggccggata cgacatggcg
      361 acccagaatg aggaagcggc gcgctacatc ggctatttgc cggaacaacc tccactctat
      421 gatgtgctcg aggtatcggc ctatttgcgc tttgtcgctc gcataaaagg tgtatcagca
      481 agatcgattc ccgccgaact ggagcgggtt attcaagcgt gccggctgga agctgtcatc
      541 acgcgtgaaa tctacaagct ctccaagggt tatcgtcagc gcgtcggact ggcgcaagcg
      601 ctgcttggca agccggaagt attgttgctc gatgaaccca ctgcgggtct tgaccccggt
      661 cagatccagg agacacgcga agtaatccgt gctttcggag agaatcacgc agtattgttg
      721 agtacccaca tccttccgga agtcaccctg atctgccagc gcgtggccat catcaaccac
      781 ggtcgtttgc ttgccatcga ttcgccggac ggattgcaac gcgcatcgga acaaaccaac
      841 tgcgtttatc tgctggccac ggggccagca gatgcgatac gcgagtcgat ccttgcgata
      901 gagggcgtaa gcgcgctgga catcaaggcg aatgcgggcc gcagcgagtt actgtccatc
      961 gaatgccagg tggacgcacg cgacggcgtg gaagccgcga tagcgcgtgc cgtagcggcg
     1021 cgctggtcac tgcaccggct tgaacgccgg cagccaacac tggaaaacat ttttctgcgc
     1081 tacatcggtg aaacatcgag cgcacgagca tccgcatgaa cggcgtattc gggatctacc
     1141 agaaagaact ggccacttac ttccgctcac cgattgcgta cttcgtgatt gcggtgttcc
     1201 tgctcggaac aggatatttt ttcatctaca acatattcct gaccggcgtt ggcaccatgg
     1261 acgagacctt ccagaacatg ggcatcctgc tgctcacgct catgccggtc atgaccatgc
     1321 ggctcttttc tggcgaatac agcggccgga ccatggaatt gctgatgact ctgcccgtca
     1381 ggccctggca aatcgtcacc ggcaagtttc tcggagcgct caccatgctg ctgttgatga
     1441 cctttgccac ggccataaac ctgataccgc tttacctgta tggaacgccc gagagttcta
     1501 ctatcctgtc cggatatctc ggtttcttgc tgcttggcat ggcgtgcctt gccgtgggcc
     1561 agtttttttc ggcgctcacg caaaatcaaa tcgtagccgc actgatcaca attcccgtat
     1621 tgctcggctt ctggttcatc ggccacgtgc agagtttcca aacctcgccg gctttgcgcg
     1681 agttgtttgg ttacctctcc ttcgccttgc attttggaga cttcattcgc ggactggtgc
     1741 gcacggaagc ggtgatgttt tatgtggtcg tcagtgccat tgccttgacg ctgaacacca
     1801 gctatttgaa gtggcggcgc tgaccatggt gcgatccttt cactccggcc ttgtactgat
     1861 cgcaggcatt gcgttgcttc tcggagccgc cgcaagccat gccatttttc tcgaaattgc
     1921 tgccgtcacc gtcattctcg ccgttgccgg cggtctgctt tcggcatggg gcgcctattc
     1981 gctgcgttct gaaatcggcg gcctgctcct gcatcgccgt ggcgaaattg tgttgtacac
     2041 gcttggcata gttggtgtac tgatcgcgtt gtcgtactta tcggtgcgct tcccggtccg
     2101 ttacgacatg acggcatcga aaatatattc tct
//
LOCUS       DATMZQ010000063         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3476254, whole genome shotgun sequence.
ACCESSION   DATMZQ010000063 DATMZQ010000000
VERSION     DATMZQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3476254"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VN496_02120"
     CDS             <1..502
                     /locus_tag="VN496_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXF15766.1"
                     /translation="SLSVAKDDMHQLKEKLEQAMKTIPAFQQMKNNVELTITNEGLRI
                     ELLENESGMFFESGNAKPSERGQEILMRLAEELGKLPNTILIEGHTDAKPFASQGQYS
                     NWELSADRANSSRRLMQNNGLRAGQLVQVRGFADQHLRTPDAPNNPSNRRISVIVRYL
                     ADKAPT"
     gene            669..1160
                     /locus_tag="VN496_02125"
     CDS             669..1160
                     /locus_tag="VN496_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXF15767.1"
                     /translation="MSTGQAEPTAENGSHPEVQGGFRMFSRVVDWITSERMQLINITD
                     RINEIVRKSGIRDGLVHLQSLHTTTAVFINEWQDALLHDVKSFLDQVVTRENGWRHND
                     PQYSDCERQNADSHLRGMLMGQTLCLQVRNSAVLLGTWQSIILAEFDGPRSRSMSILV
                     SGV"
     gene            1194..1634
                     /locus_tag="VN496_02130"
     CDS             1194..1634
                     /locus_tag="VN496_02130"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HXF15768.1"
                     /translation="MPKRFTLPEAESLLPELEKVMREAISLKLEFEEAESSVQALTQR
                     VIMMGGVLVDRQAVLENKHRRDRTAARLKTSLEKIQEFGCVIKDLDIGLVDFPTEFRG
                     QEVYLCWKLGETGIQFWHGVHEGFAGRKPIDQDFLEHHKGDAAN"
     gene            complement(1631..>2370)
                     /locus_tag="VN496_02135"
     CDS             complement(1631..>2370)
                     /locus_tag="VN496_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXF15769.1"
                     /translation="FMISLLWVMVGYSGWNAATYIAEEVKKPERTLPMALAVGTCLVT
                     ALYLGLNLIFIYATPLESMKGVLAVGALSASNLFGPQVAGVFAALMAIGITSTVSAEV
                     TVGPRVYYAMAKNRAFFKIAAWVHPRWHTPVPAIVAQGLCAMLMTLTPFPQLVVYIGF
                     SLTFFTVMSVASLFVLRRRQPKWQTLRPVSFLYPLIPGAYILVGAGMITYGVIWQPKA
                     SLTALATIAVGAAVYHFGPAKALARDA"
BASE COUNT          554 a          664 c          722 g          430 t
ORIGIN      
        1 aagcttgagc gtcgcgaaag acgacatgca tcagctcaag gaaaaactgg agcaggccat
       61 gaagacgatc cccgcgtttc agcagatgaa gaacaatgta gagctgacga taaccaacga
      121 agggttgcgc atcgagctgc tggaaaatga atccggcatg tttttcgaaa gtggtaacgc
      181 caagccgagc gagcgtggcc aggagatcct gatgcgcctc gccgaggagc tggggaaatt
      241 gccgaacaca atcctgatcg agggacacac cgacgccaag ccctttgcca gccaagggca
      301 gtacagcaac tgggagctgt cggctgatcg ggctaactcc tcgcgcaggc tcatgcaaaa
      361 caacggcctg cgcgcgggcc aactcgttca ggtgcgcggt ttcgccgacc agcatctgcg
      421 cacgccggac gctccaaata atccgtccaa tcggcgtatt tcagtgatcg tcagatacct
      481 tgccgacaaa gcgccgactt aagcaaccca ggaaccggcc ccggcgaata cagtcgcgca
      541 tccggaaccc gccgccgcgc ctgagataca tcgccgcggg ccgcctgcaa tggggttcgc
      601 gcgcgcttgc tataatcgtt tggaaccgca cgagggacct ttggaactct aactggaaag
      661 gcacacagat gagcacagga caagcggagc cgactgcgga gaacgggtct catcccgagg
      721 tacaaggcgg gttcagaatg tttagccgcg tagtggactg gatcaccagc gagcggatgc
      781 aactcatcaa cattacggat cgcattaatg aaatcgtgcg gaaaagcggt attcgcgatg
      841 gtttggttca tctgcagtcc cttcacacaa ccacggcggt tttcatcaat gaatggcagg
      901 atgctttgct gcacgatgtg aagtcatttc tcgaccaggt ggtcacccgc gaaaacgggt
      961 ggcggcataa tgatcctcag tattccgact gcgagcgcca gaacgcggac tcgcatctgc
     1021 ggggcatgct gatgggacag actttgtgtt tgcaggtccg caattccgct gtcctgctgg
     1081 gcacctggca gagcatcatt ctggccgagt tcgacgggcc gcggagccgt tcgatgtcca
     1141 ttctggtctc gggcgtttag ccatcccagg ggcggcggag acgcctcaac ttcatgccga
     1201 aacgcttcac gcttccggag gcggaaagtc ttctgcccga actggagaag gtcatgcgcg
     1261 aagcgatctc actcaagttg gaattcgagg aagcggaaag cagcgtacag gcgctcaccc
     1321 agcgtgtgat catgatgggc ggagtgctgg tggaccgtca ggcggtgctc gagaacaagc
     1381 acaggcggga ccgcacggcc gcgcggctga agacctcgct cgagaagatt caggagttcg
     1441 gctgcgtgat aaaggatctg gacatcgggc tggttgattt tccaacggag tttcgcggac
     1501 aggaagttta cctctgctgg aaactgggcg aaaccggcat ccagttttgg cacggcgtgc
     1561 atgaaggctt tgccggacgc aaaccgatcg accaggattt tctggaacac cacaagggag
     1621 acgcggcaaa ctaggcgtcg cgcgcgaggg ctttcgccgg cccgaaatga tagaccgcgg
     1681 cacccacggc gatggtggcc agagcggtca gcgatgcttt cggctgccag atgactccat
     1741 aggtaatcat gcccgcgccc accagaatgt aagctccggg gatcaaggga taaagaaaac
     1801 tgaccgggcg cagcgtctgc catttcggtt gccggcggcg caatacgaac aggcttgcta
     1861 ccgacatgac ggtgaagaac gtcagactga acccgatgta caccaccagt tgcgggaaag
     1921 gcgtaagagt catgagcatg gcgcaaaggc cctgcgcgac aattgccggc accggggtat
     1981 gccaccgcgg gtgtacccag gcggctattt tgaaaaaggc gcgatttttg gccatggcgt
     2041 agtagacgcg cgggccgacg gttacctccg cgctgaccgt cgacgtgatg ccaatcgcca
     2101 tgagcgccgc aaagacgccg gccacctgtg gtccaaaaag gttggatgcc gaaagcgcgc
     2161 cgaccgcgag cacgcctttc atcgactcga gcggcgtggc gtaaatgaaa atcagattga
     2221 gcccgagata aagcgctgtc accaggcatg ttcctacagc gagcgccatg ggcaatgttc
     2281 gctcgggctt tttcacttcc tccgctatgt aggtggcggc attccaaccg ctatagccga
     2341 ccatcaccca cagcaagctg atcatgaatt
//
LOCUS       DATMZQ010000064         6695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_714361, whole genome shotgun sequence.
ACCESSION   DATMZQ010000064 DATMZQ010000000
VERSION     DATMZQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714361"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VN496_02140"
     CDS             <1..941
                     /locus_tag="VN496_02140"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXF15770.1"
                     /translation="ARPLIAKRLIEIARKTGADAISHGATGKGNDQVRFELGAYALMP
                     DVKVIAPWREWDLLSRDKLLAYADEHGIPVDFKKRKGGGAPYSMDANLLHISYEGGIL
                     EDPDFEPEESMWRLTVSPEKAAARPEHVELEYRKGDITAINQKRMTPAQVLTKLNEIG
                     GKHGIGRLDIVENRYVGMKSRGCYETPGGTIMLKAHRAMESITLDREVLALKDDLMPR
                     YARMIYNGYWFSPERLLLQSLIDQSQEPVNGTVTLKLFKGTVMVVGRASKSDSLFDSR
                     IATFDDDKGAYNQADAAGFIRLNALRLRIGAKRRTK"
     gene            1003..1488
                     /locus_tag="VN496_02145"
     CDS             1003..1488
                     /locus_tag="VN496_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HXF15771.1"
                     /translation="MPSFDIVSEVNQVELRNALDQTNKEVTNRFDFKGSDARVEHAED
                     VLTIFADDEFKLGQVYDVLIGKLTKRGVDIRCLQRGKTETISGDKIKQAITVKSGVDQ
                     ELGKKLQKLIKDARLKLQASIQGDAVRVSGAKKDELQQAIALVRKQVTDFPIQFGNFR
                     D"
     gene            complement(1501..2211)
                     /locus_tag="VN496_02150"
     CDS             complement(1501..2211)
                     /locus_tag="VN496_02150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HXF15772.1"
                     /translation="MQPVAIFRHWPTEGPGYFATYLDSHDIPWRLVALDEGQSVPQGP
                     GDFGGLVFMGGPMSVNDGLSWIAPVLDLIRKAVAADIPVLGHCLGGQLMAKALGGSVG
                     RNPVKEIGWGEVEVADNGEARAWFGPDLTRFEAFHWHGETFTLPPGAVRIMSNRSCAN
                     QGFVLGRHLAMQCHVEMNSDLINSWCESGANEIAASKSPAVQSPDQMRSGLEARLVTL
                     HAVAMQLYGRWTENLKKN"
     gene            2223..2876
                     /locus_tag="VN496_02155"
     CDS             2223..2876
                     /locus_tag="VN496_02155"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HXF15773.1"
                     /translation="MRINAANITRVLAGLVLLCAPAWALDVSVVALFPGKAMLIIDKS
                     KPRTLRAGETYAGVTLVSSTSDEAVISVNGKEQRLHIGEGVYSALSVQNQHATVALTP
                     DKNGHFISSGSINGASVSFLVDTGATMVAMNVDEARRAGVNYLAGERGVSQTANGITP
                     IYRVKLAQVTLGDITLRDIDGVVHENSALPMVLLGMSFLGKLEMRRDGDSLILAKRY"
     gene            2911..3237
                     /locus_tag="VN496_02160"
     CDS             2911..3237
                     /locus_tag="VN496_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXF15774.1"
                     /translation="MIYELRIYHAFPGKLPALNARFSNHTLKLWERFGIRQVGFWTTL
                     VGDSNNDLYYILEWENLAEREQKWNAFATHPDWLATRAETEKDGPLTTHITNLILTPT
                     AYSKMR"
     gene            3329..4432
                     /locus_tag="VN496_02165"
     CDS             3329..4432
                     /locus_tag="VN496_02165"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXF15775.1"
                     /translation="MDERLVYNVNVASQDVLLNPDEIKRRVPLTEKAEQTVLEGRHAL
                     ERILDRRDHRLLVVVGPCSIHDPVAAMDYARRLKKIADEVADTMLVVMRVYFEKPRTT
                     TGWKGLINDPHMDDSFDIEKGLELARRVLRDINELGLPAGTEALDPISPQYLGDLVTW
                     SAIGARTTESQTHREMASGLSTPVGFKNGTDGGLQVAINALLSVSRPHSFLGINQRGQ
                     VSVIRTSGNRYGHIVLRGGAKGPNYDSVTMAVVEKELGKNKLAANIVVDCSHANSNKD
                     HNLQPLVMLDCAHQIMEGNQSIVGVMLESNINAGNQAIPPDLKQLKYGISVTDACIDW
                     ATTEKLLRDARGKLKDALPKRIPRTAEIRRIGS"
     gene            complement(4504..5982)
                     /locus_tag="VN496_02170"
     CDS             complement(4504..5982)
                     /locus_tag="VN496_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXF15776.1"
                     /translation="MQKYQMYIGGEWVAPSSGEWFESYNPFTGEPWAMIPRGNAADAD
                     HAVRTAHAAFTDGPWPQMTPTQRGALLRKLGDLVAQNAQQLAEIEVRDNGKLIAEMAG
                     QLNYVPQWYYYFGGLADKVQGAVIPLDKKGYFNFTRYEPLGVVVAITPWNSPLMLTAW
                     KLAPALAAGNTVVIKPSEFTSASALEFVKLVEQAGFPAGVINVVTGYGKEAGMPLVEH
                     PLTAKITFTGSDATGRVIAEAAARGFKKVGLELGGKSPNIVFEDAVIEDAVNGAVSGI
                     FAATGQTCIAGSRLLLQQSIHDEFVDKLLSLAKTAKMGNPMSLETQVGPVTTQPQYQK
                     VLSYIDVAKNEGAKLLMGGGPAKRPECGNGWFVEPTIFTGVDNKMRIAQEEVFGPVLS
                     IIPFRDEEDAVRIANDVRFGLGAGVWTRDVGRAIRMSERIKAGTVWVNTYRAVSFMSP
                     FGGYKDSGVGRENGQSAIYEYLQMKSVWINTGVKTGNPFVMR"
     gene            complement(6020..6448)
                     /locus_tag="VN496_02175"
     CDS             complement(6020..6448)
                     /locus_tag="VN496_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXF15777.1"
                     /translation="MRANIVAMLNYPLVRFVLFWGVNTLSLWVADDLFEAISFATPQS
                     LFIAGLMLGVMNAFLKPLLVILTLPLSIITFGLFVLIINAMNHQCNDAAAGSVAGTRF
                     RRRRILVRIYGCDFRVGFQFHPEFHDWLQQDQDSTHAIRR"
BASE COUNT         1535 a         1913 c         1851 g         1396 t
ORIGIN      
        1 tcgctcgtcc attgattgcc aagcgcttga tagagatcgc ccgcaagacc ggcgccgacg
       61 ccatctcgca tggcgcaaca ggcaagggca atgaccaggt ccgctttgaa ctcggcgctt
      121 atgcactcat gccagacgta aaggtcatcg cgccgtggag ggaatgggat ctgttgtcgc
      181 gggacaagct gttggcatat gccgacgagc atggcattcc agtggatttc aaaaagcgca
      241 agggcggcgg tgcgccgtat tcaatggatg ccaacctgct ccacatttca tatgaaggcg
      301 gaatcctgga ggatcccgac ttcgaaccgg aggaatcaat gtggcgcctt acggtgtcgc
      361 ccgaaaaagc ggcggccagg cccgaacatg tcgaactgga ataccgcaag ggcgacatca
      421 cagccatcaa tcaaaaaaga atgacacccg cgcaagtgct taccaaattg aacgagattg
      481 gtggcaagca cgggatcggg cggctggaca tcgttgaaaa ccggtatgtc ggcatgaaat
      541 cccgcggctg ctacgagaca ccgggcggca ccatcatgct caaagcacac cgcgcaatgg
      601 agtccatcac gctcgaccgc gaagtgcttg cgctgaagga cgatttgatg ccgcgttatg
      661 cgcgcatgat ttataacggc tactggttca gtccggagcg cctgctgctg cagtcgctca
      721 tcgaccaatc gcaggagccg gtcaatggta ccgtcacgct caagctcttc aagggaactg
      781 tgatggttgt cggccgggca tcaaaatccg attcgctgtt cgattcgcga atcgccacct
      841 tcgatgatga caagggagcc tacaatcaag cggacgccgc tggctttatc cgcttgaacg
      901 ccttgcggct gcggattggc gccaagcgcc gcacgaaata gcagatgttt cattcaaggc
      961 cgttcccgag cagtcaggca aagcatttcg atgaggtgcg ccatgcccag ttttgacata
     1021 gtgtccgaag tcaatcaggt ggaactgaga aacgccctgg atcagaccaa taaggaagtt
     1081 accaaccgct tcgacttcaa gggatccgat gcccgtgtgg aacatgcgga agacgttctg
     1141 accatttttg cggatgacga attcaaactc gggcaggttt atgacgttct catcggcaaa
     1201 ctcaccaaac gcggcgtaga catccgctgc ctgcagcgcg ggaaaacgga aacaatcagc
     1261 ggcgacaaga taaagcaagc catcaccgta aagtcggggg tggatcagga gcttggcaag
     1321 aagttgcaga aactcatcaa ggatgcccgg ctcaagttac aagccagcat ccagggcgat
     1381 gcagttcgtg tgtcgggtgc aaaaaaagac gaattgcaac aggcgatagc gcttgtgaga
     1441 aagcaggtaa ccgacttccc gattcaattt ggtaacttca gggactgaac tgcagcattc
     1501 tcagtttttt ttgagattct ccgtccaacg tccgtagagc tgcatggcga cggcgtgcag
     1561 cgtcacaagc ctggcttcca gtccactgcg catctgatcc gggctctgca ccgcgggact
     1621 cttcgacgcc gcgatttcat tcgctccaga ttcacaccag ctattgatga gatcgctgtt
     1681 catctcgacg tggcactgca tcgcgagatg gcgccccagg acaaagccct ggtttgcgca
     1741 actacgatta ctcatgatgc gcactgctcc cgggggaagc gtgaacgttt cgccatgcca
     1801 gtgaaatgcc tcaaaacggg tgagatcggg gccgaaccac gctcgtgcct cgccgttatc
     1861 ggctacttca acttcgcccc agccgatctc ctttacagga ttgcgcccaa cgcttccgcc
     1921 aagcgccttg gccatcagct gcccgcccaa acaatgtccc agaaccggga tgtctgcggc
     1981 caccgccttg cgaatcaagt caagtactgg cgcgatccac gacaagccgt cgttaacgct
     2041 catcggacca cccatgaaca ctaatccgcc gaagtcgccg ggtccttggg gaacggactg
     2101 tccctcgtca agcgctacca atcgccacgg aatgtcgtgc gaatcgagat acgtggcaaa
     2161 atagcccggc ccttctgtgg gccagtgacg gaatatcgca accggttgca tatgggagtc
     2221 tcatgcgcat caatgccgca aatattacgc gggttctggc aggccttgtc ctcttgtgtg
     2281 ctccggcgtg ggcgctggat gtcagcgtcg tggcgctgtt ccccggcaaa gccatgttga
     2341 tcatcgacaa gagcaagcca cgtaccctgc gtgcgggtga gacgtacgcc ggcgtgacgc
     2401 tcgtcagttc gaccagcgat gaggcggtca tttcagtcaa tggcaaggag cagagattgc
     2461 atatcggcga gggagtctac agcgcgctgt cggtgcagaa tcagcatgca acagtggcgc
     2521 tgacgccaga caagaacggg cattttattt cctccggcag catcaatggg gcgtcggtca
     2581 gcttcctggt agacaccggc gccaccatgg ttgcaatgaa cgtggatgag gctcggcgtg
     2641 ccggtgtcaa ttacctcgcg ggcgagcgcg gtgtatcgca aacggcaaac gggatcacgc
     2701 cgatctatcg agtcaagctc gcccaagtca cgctgggtga tattactttg cgcgacatcg
     2761 acggcgtcgt gcatgaaaac agcgcgctgc cgatggtgct gctgggcatg agtttccttg
     2821 gcaagctcga aatgcggcga gacggggaca gcctgatcct cgccaagcgc tactgatacc
     2881 ctaacgtaaa tcaaccagaa gaaagacaga atgatctacg aattgcgcat ttatcatgca
     2941 ttcccgggca aacttcccgc gctcaatgca agattctcca atcacacgtt gaaattatgg
     3001 gagcggttcg gcatacgcca ggttggtttc tggacgaccc tggtcggcga cagcaacaat
     3061 gatctgtatt acatcctgga atgggaaaat cttgcagaac gggagcagaa atggaatgcg
     3121 ttcgcaaccc atcctgactg gctggcgact cgggcggaaa ccgaaaagga cggcccgctg
     3181 acgacccata ttaccaacct gattttgacg ccgaccgcct attcgaagat gagatgaccg
     3241 tgttgcaggc tgcgcagcgg catccggcta tgacgatacg gcgtgattcc gtataattgc
     3301 gcgcctcttg agcatcggcc cagtaatcat ggatgaaaga ctggtttata acgtaaacgt
     3361 cgcgtcacag gacgtcctgc tgaacccgga tgaaatcaag cgccgggttc ccttgacaga
     3421 aaaggcagag cagacagttc tcgaaggcag gcatgccctc gaacgcattc tcgaccgtcg
     3481 cgaccatcgc ctgctggtgg tggttgggcc gtgttccatc cacgatcccg tggcggccat
     3541 ggactatgcg cggcggctga agaaaatcgc cgatgaagtc gctgacacga tgctcgttgt
     3601 catgcgcgtg tactttgaaa aaccgcgtac cacgaccggg tggaaaggct tgatcaacga
     3661 ccctcacatg gacgactcgt tcgacatcga gaagggtctg gaactcgcgc gcagggtatt
     3721 gcgcgacatc aatgagttgg gcttgccggc cggtacggaa gcactcgatc cgatcagccc
     3781 gcaatatctg ggtgatctgg ttacctggag tgcaatcggt gcgcgcacaa ctgaatcgca
     3841 gacacaccgg gaaatggcga gcggcttgtc cacgccagtc ggattcaaga acggcaccga
     3901 cggcggtttg caggtggcaa tcaatgcgct gctatctgtt tcccggccgc acagttttct
     3961 cggcatcaac cagcgtggcc aggtatccgt catccgaacc agcggcaacc gctacggaca
     4021 catcgtgctg cgtggggggg cgaaagggcc aaactacgat tccgtaacca tggctgtggt
     4081 tgaaaaggaa cttggcaaaa acaaacttgc agccaacatc gtggtcgact gcagtcatgc
     4141 caattcgaac aaggatcaca acctgcagcc attggtgatg cttgactgcg cccaccagat
     4201 catggagggc aatcaatcca ttgttggcgt catgctcgaa agcaacatca acgcgggcaa
     4261 ccaggcgatt cccccggact tgaagcaact gaagtacggg atatcggtga ccgacgcctg
     4321 cattgactgg gcaaccaccg aaaaactttt gcgcgatgcg cgtggcaaac tcaaggacgc
     4381 gctgccgaag cgcattccac gaaccgccga aattcgacgg atcggaagct gatcaattta
     4441 cgactttcga aaagaaacgg ggcgctaaag gcgccccgtt tcatgtctgt aaactaaatt
     4501 cagctatcgc atcacaaacg gattgcctgt cttgaccccg gtgttgatcc acacgctctt
     4561 catctgcaga tattcgtaga tggcgctctg cccgttctcg cgccctacgc cgctatcttt
     4621 gtagccaccg aaaggcgaca tgaagcttac cgcccgatag gtgttgaccc ataccgtgcc
     4681 ggccttgatg cgctccgaca tgcggattgc cctgcccaca tcgcgggtcc atacgcctgc
     4741 accgaggcca aatcgcacat cattggcaat gcgcactgca tcttcctcat ccctgaacgg
     4801 aatgattgat aagacaggtc cgaatacctc ttcctgtgca atgcgcatct tgttgtccac
     4861 gccggtgaag atcgtcggct cgacgaacca cccgttgcca cattccggac gcttcgccgg
     4921 ccctcctccc atgagcaact ttgcgccttc attcttggcg acgtcgatgt acgacaagac
     4981 cttctggtat tgcggttgcg tggtcaccgg ccccacctgc gtttccagac tcatcggatt
     5041 acccattttt gccgttttcg ccagcgacag cagtttgtcg acgaactcat catgaatgga
     5101 ttgctgcagc aacaggcgag agcccgcgat gcatgtctgc cctgtcgccg cgaatattcc
     5161 cgatacggcg ccgttaactg cgtcctctat gacagcgtcc tcaaacacta tattgggcga
     5221 cttgccgcct aactccaggc ccactttctt gaagccacgt gccgccgcct ctgcaatcac
     5281 gcggccggtt gcgtcagatc cagtaaaagt gatcttcgcg gtcagcggat gctccactag
     5341 cggcatgccc gcttccttgc cgtagccggt gacgacgttg ataacgccgg cgggaaaacc
     5401 ggcctgctcg accagcttga cgaattcgag cgcggatgcc gacgtgaact cggacggttt
     5461 gatgactacg gtattgcccg ccgccagcgc gggtgcaagc ttccaggcgg tcagcatcag
     5521 cggggaattc cacggcgtaa tcgccacgac tacgccaagc ggttcgtacc gggtaaagtt
     5581 gaaatagccc ttcttgtcga gcggaatgac cgcgccctga accttgtcag ccagcccgcc
     5641 gaagtagtaa taccactgag gcacgtaatt aagttgtcct gccatttcgg caattagctt
     5701 gccgttgtcg cgcacttcga tttccgcaag ctgctgcgcg ttttgcgcga ccaaatcgcc
     5761 cagcttcctc agcagtgcgc cacgctgcgt cggtgtcatt tgtggccacg gaccatcggt
     5821 gaacgcggcg tgcgctgtgc gcacggcgtg gtccgcgtcc gcggcattgc ctctgggaat
     5881 catcgcccat ggttccccgg taaacgggtt atagctttcg aaccactcgc ccgaagaagg
     5941 cgcgacccac tcgccgccga tatacatctg gtacttctgc atgtgtatct ctcctgctgt
     6001 cttttgagtt accgcatctt catcgcctta ttgcatgcgt tgaatcttga tcttgttgta
     6061 gccaatcatg gaattcagga tgaaactgaa aaccgacacg aaaatcgcaa ccgtaaatcc
     6121 ggaccagaat cctgcgacga cgaaaccggg taccagccac gctaccagca gcagcatcat
     6181 tgcattgatg attcattgca ttgatgatta gcacgaatag tccgaatgtg atgatcgaca
     6241 acggcagggt aagtatgacc agcaagggtt tcaggaatgc attcatcaca ccaagcatca
     6301 ggccggctat gaaaagcgat tgcggtgtcg cgaacgagat agcctcgaac agatcgtctg
     6361 ccacccagag agaaagcgta ttgacgcccc agaacagcac gaagcgcacg agcggataat
     6421 tcagcatggc gacgatgtta gcacgcaccg cattttcgag gcgtcgcgcg gtgctggttc
     6481 gctcaccgtt acatctgcgt tgagccccgg taagtctgcg ctgtttgcct tgctgtacac
     6541 ttcgcgattt ttcgagaatc cttcatgccg acccaatatc acaccttgcc gcgttcaatg
     6601 tgggtgctgc tggttttatt gacgtttggc tggggattga actggccaat gatgaaaatg
     6661 acgttggccg agttgccggt ctggacattt cgggg
//
LOCUS       DATMZQ010000065         3532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4167053, whole genome shotgun sequence.
ACCESSION   DATMZQ010000065 DATMZQ010000000
VERSION     DATMZQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4167053"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VN496_02180"
     CDS             complement(<1..888)
                     /locus_tag="VN496_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXF15778.1"
                     /translation="MHCAPSRLCNVVFVLCCILCGAGQPALAQHYPSKPIRIIVPYPP
                     GGFNDTLARTLGQRLTESWHQAVIVDNRPGAGSTIGTNLAAKAPADGYTLLITSFAFA
                     VNPALYSSLPYDSERDLLPVVLAATTPNLLVVNPQLPVQSIKDLIALAKANPGKLNYA
                     SAGNGSSNHLSMELFKMLTGTDMVHVAYKGSAPAVNDLMGGQVNLMFDNAPNVLQQVR
                     AGKLRALAISSKQRSPIVKELPSVAEAGVPGFDVSVWFGVMAPAGTPRAVIHQLNAQI
                     NAILQLPQIRQAFASQGVET"
     gene            complement(926..1687)
                     /locus_tag="VN496_02185"
     CDS             complement(926..1687)
                     /locus_tag="VN496_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXF15779.1"
                     /translation="MADTVQLQRRETVAIIILNRPEVLNAVNVAMRETLIGLLRELNS
                     DDATRAIVITGAGERAFCSGQDLDETSKYGVDDVDAWLTRQHAMYGAVRDLDKPCIIA
                     FNGTAAGAGFQIGLCADLRVGYPEMKLGQPEIKAGLASIVGSYLMSLHVGLSRNLELS
                     LTGELISGENAHAIGILNHLVRKESVFEKSCALATEMTKLPVTAMRLTKQRFRNVTQA
                     GFESALVEAKAAQKQAYASGEPQTAMRRFLAARNK"
     gene            complement(1705..2853)
                     /locus_tag="VN496_02190"
     CDS             complement(1705..2853)
                     /locus_tag="VN496_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXF15780.1"
                     /translation="MTGPRITEVRAYPTSFPVNPKDSVTLGIGRAVKRDAVIVKVSTE
                     DGLIGWGEAHHGRCPGAVAHLVNTTMRQLVLGQDANDITGVWKKVYDKQLASHGMGAG
                     ACLALSGIDMALWDIRGKMLKLPLYQLLGGRSRPIPAYAGGVSLGYQDPASLAEEAQP
                     YVRAGYKAIKLRIGDSVSRDIERVAMVRKTFGSSLAILSDANTGYTVADARAAMPALD
                     ELNVGWLEEPFPAHDYRSYREAARYGRLPLAAGENHYTRFEFNRVIEDGAITILQPDL
                     SKTGGITEALRIAAMASSWKLPIHPHTSMTGLNMAATIHFLAAIDNGGYFEADISKNN
                     LFRDKLVSTPYEIDAEGNVLPLEADGIGVELDEGFLQAHPVIEGPAYV"
     gene            complement(2878..3393)
                     /locus_tag="VN496_02195"
     CDS             complement(2878..3393)
                     /locus_tag="VN496_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15781.1"
                     /translation="MKFVNQLLSLHLKRYPAMQLDDIYKLLHQAALGPGHAVDNPAAA
                     RKRLDEETATLASGATEPAQDIISPDGRLGRVHLRTYLAQGGDLGTLHHAFVETANSY
                     PASPDKLAKFCGCLGDLAAAGGIPFAQEDVVAYFEKIAQDRYPVVHHSATFRDAYHPA
                     YRVIAVDLLKK"
BASE COUNT          718 a         1009 c         1031 g          774 t
ORIGIN      
        1 agtttcaacc ccctggctgg cgaaggcctg gcggatttgt ggaagctgca ggatggcgtt
       61 gatctgtgcg ttgagttgat ggatgacagc gcggggtgtc cccgcggggg ccatgacacc
      121 aaaccagacc gatacgtcaa atcccggcac gcccgcttcc gcgacactcg gcaattcttt
      181 gacaatgggt gagcgctgct tgcttgatat ggccagcgcg cgcagcttgc cggctcttac
      241 ttgctgaagc acgttaggcg cattatcgaa catcagattc acctggccgc ccatgagatc
      301 attcacggcc ggcgcgctgc ccttgtatgc aacatgaacc atgtccgtcc cggtcagcat
      361 cttgaacaac tccatcgaaa gatgattcga cgagccgttg ccggctgaag cgtagttcaa
      421 cttaccgggg ttagccttcg ccaacgcaat gagatccttg atcgactgta ccggcagctg
      481 gggattcacg accaacaggt tcggtgtggt tgccgccagg acaacgggca acaagtcgcg
      541 ttccgagtcg tagggcaacg aactatagag cgcgggattg accgcgaacg caaaagaagt
      601 gatcagcagc gtgtatccat cggccggcgc ctttgccgct aaattggtgc cgatggtgct
      661 gccggcaccg ggacggttgt cgacaatgac agcctgatgc cacgactccg tgagtctctg
      721 ccccagcgtg cgtgccagcg tgtcgttgaa tccgccgggc gggtacggga cgatgatcct
      781 gatcggcttg ctcggatagt gctgtgctaa agcaggctgc ccagctccgc agagaatgca
      841 gcacagaaca aaaacgacat tgcaaaggcg ggatggtgca cagtgcattg ttggtcaatc
      901 gcgtcagatc gtgcctgtaa gcgagctatt tgtttcgagc ggccagaaag cggcgcatgg
      961 cggtctgcgg ctcgccgctg gcataggctt gcttctgagc tgctttcgct tccaccagtg
     1021 cggattcaaa gcctgcttgc gtgacgttgc gaaagcgctg cttggtgagc cgcatggccg
     1081 ttacaggcag ctttgtcatc tccgtggcga gagcgcatga tttttcgaat acgctttcct
     1141 tacgcaccag gtgattgaga ataccaattg cgtgcgcgtt ctcgcctgaa atgagctcac
     1201 cagtaagcga tagttccaga ttcctggaaa gtcccacatg caaactcatt aaataggagc
     1261 cgacgatgct ggccagtccc gccttgattt ccggctggcc caatttcatt tccggatagc
     1321 caacgcgaag atcggcgcac aggccgatct ggaatccagc acctgcagcg gtgccgttga
     1381 acgctatgat gcagggcttg tccaggtctc gaacggcgcc gtacatcgca tgctgccgcg
     1441 tcagccaggc atcgacatca tcgacgccgt attttgaagt ctcgtccaga tcctgtcccg
     1501 agcaaaatgc ccgctcaccg gcgcccgtaa tcacgatcgc gcgtgttgca tcatcgctat
     1561 tcaattcacg cagcagcccg atcagcgttt cacgcatcgc aacgttcacg gcgttcaaga
     1621 cttccggccg gttgaggata atgattgcaa ccgtctcccg gcgttggagc tggaccgtgt
     1681 cagccatgtg cgatgcattc atggtcagac ataggctggt ccttcgatga cggggtgggc
     1741 ctgcaggaac ccctcgtcca gctcaacgcc gataccatca gcttccagtg gcagcacgtt
     1801 gccctcggcg tcaatttcat aaggcgtcga gaccagcttg tcgcggaaca gattattttt
     1861 cgatatgtct gcctcgaagt acccgccgtt gtcgatggca gccagaaaat gaatcgtggc
     1921 ggccatgttg agtcccgtca tggaggtatg cggatggatc ggcagtttcc agcttgacgc
     1981 cattgcggca atgcggagcg cttccgtaat gccaccggtt tttgaaagat cgggctgcag
     2041 gatggtgatg gcgccatcct cgatgacccg gttgaactca aaccgggtgt agtggttttc
     2101 accggctgca agtggcagac ggccataacg agccgcttca cgataggagc ggtagtcatg
     2161 tgcgggaaaa ggctcctcga gccaaccgac attcaactca tccagcgccg gcattgcggc
     2221 gcgcgcgtca gccactgtat agccggtgtt ggcgtcggag agaattgcga ggctgctgcc
     2281 aaaggttttt ctgaccattg caacccgctc gatgtcgcgc gacaccgaat cgccgatgcg
     2341 taatttgatc gccttgtagc cggcccgcac ataaggctgt gcttcctctg ccagtgatgc
     2401 cggatcctga taacccagcg atactccacc tgcataggcc ggaatcggcc tggaccggcc
     2461 gccgagtaat tgatacaacg gtagcttgag catcttgccg cgaatgtccc acagcgccat
     2521 gtcgatgccg gaaagtgcaa ggcacgctcc tgcacccatg ccgtggctag ccagctgctt
     2581 gtcgtagact ttcttccaga cacccgtgat gtcgttggca tcctggccca acactaactg
     2641 gcgcatcgtc gtgttgacca ggtgtgcgac agcgccaggg catcgtccat ggtgcgcttc
     2701 gccccagcca atcagcccgt cttcagtcga gaccttgacg atcaccgcat cgcgtttaac
     2761 ggcgcggccg atgcccaatg tcacgctgtc tttcggattg actggaaatg aagtcggata
     2821 tgcccgcacc tcggtaatgc gtggccctgt cataagactt cctccctatt ctgctattta
     2881 ttttttcagt agatcgaccg caatgacgcg atatgcgggg tgatacgcgt cgcggaaggt
     2941 cgccgaatga tgaactactg gatagcggtc ctgcgcgatt ttttcgaagt atgcgacgac
     3001 atcttcctgt gcgaacggta tgccgccagc tgccgcaagg tcgccaaggc agccgcagaa
     3061 tttcgccagc ttgtcgggcg aggccggata gctgtttgcg gtttcgacaa aggcatgatg
     3121 cagcgtgccg aggtcaccgc cctgcgccag gtaggtgcgc aggtggacac ggccgagccg
     3181 gccgtccggc gaaataatgt cctgcgccgg ctcggtggcg ccgctggcca gcgtggccgt
     3241 ttcctcatcg agccgcttgc gcgctgcagc aggattgtct actgcgtggc ccggacccag
     3301 cgcagcttgg tgtagcagct tgtatatgtc atcaagttgc attgcaggat aacgcttgag
     3361 atgcagagac aacaactgat tgacgaattt catctggcgc tccggaatga tgcagcatct
     3421 taatccatcg agccgtctcg gattgaagcg ggttgcaaag agcgtgtcgt aagattaagt
     3481 cgggattcac ttcttcgcgc tgggaaactg gcaactttgc tgcatgacgg cc
//
LOCUS       DATMZQ010000066         5164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2095831, whole genome shotgun sequence.
ACCESSION   DATMZQ010000066 DATMZQ010000000
VERSION     DATMZQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095831"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2122
                     /gene="leuS"
                     /locus_tag="VN496_02200"
     CDS             <1..2122
                     /gene="leuS"
                     /locus_tag="VN496_02200"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXF15782.1"
                     /translation="KTGIVNWDPVDQTVLANEQVIEGRGWRTGALVEKREIPMYYLAI
                     TKYAEQLLSSLDSLPGWPERVKTMQANWIGKSYGVRAAFPYVLNGKPEKLWVFTTRAD
                     TIMGVTFCAVAAEHPLAAAAAQRNSGLAEFVEECRRGSIMEADLATMEKKGMRTGLFV
                     NHPLTGKKIEVWVGNYVLMSYGEGAVMGVPGHDERDFAFAKKYGLPILQVIDVPGKRY
                     DVDAWQPWYADQGLCINSGKYDGLDFVRSVDAIAADLNAEGCGEKQVTWRLRDWGISR
                     QRYWGCPIPIVHCAACGDVPVPEDQLPVLLPEDCVPDGSGNPLNKRADFVNTACPKCA
                     RPAKRETDTMDTFVDSSWYFLRFACPDQHSKMVDERVNYWLPVDQYIGGIEHAILHLL
                     YSRFWTKVMRDLGLVKFDEPFTHLLTQGMVLNEIFQRKVDGGRVQYFNPSDVEIEFDD
                     QGKRSRTILKSDGRPVESGGIRTMSKSKNNGVDPQALIDQYGADTARFFMMFTSPPED
                     TLAWNDDGVEGAYRYLRRLWKFNYEHHTAISDSAASVTAADRNYRRELHLLLQQANFD
                     YERKQFNTVASASMKILNLLEQAGAGKAGLLREGMSILLRLLSPIAPHIAQTLWRECR
                     FGDDILNADWPVVDTAALVREQIELVLQVNGKHRGSVVVPSDASRAAIEDLALASPNV
                     QKHIAGLPVKKVIVVPGRLVNIVV"
     gene            2139..2663
                     /gene="lptE"
                     /locus_tag="VN496_02205"
     CDS             2139..2663
                     /gene="lptE"
                     /locus_tag="VN496_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HXF15783.1"
                     /translation="MCKRYSLRDPGLSALTTAIIAVAMILSSCGFQLRGQAAIPYKTL
                     FIETSGYSQFANDLERAIRFSSGTKVVSNRDDAQAILRIMGEAQEKHILSLSSGGRVR
                     EFELNYRVNYRLIDHAGKDLAQPGQIDLRRDMTYDDTEVLAKESEEQLLYRDMKKDAV
                     QQMMRRLSAAKPVA"
     gene            2713..3852
                     /locus_tag="VN496_02210"
     CDS             2713..3852
                     /locus_tag="VN496_02210"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXF15784.1"
                     /translation="MPPEGSVGVVSPRLAHFNDPLALRSGARLEAYDLAYETYGTLNS
                     KRSNAVLVCHALNASHHVAGSYADDPGNVGWWDNLIGPGKPVDTDRFFVVGVNNIGGC
                     HGSTGPASVNASTGQPWGAAFPVVTVEDWVEAQARLATRLGIDRFAAVMGGSLGAMQA
                     MQWAISQSSRVGHSIVIAAAPKLSAQNIAFNEVARQAITTDPDFHGGNYYSHGVVPKR
                     GLRIARMIGHITYLSDDAMAEKFGRERRAGALQFNFDVEFQIESYLRYQADKFAERFD
                     ANTYLRITKALDYFDPAAAHGGILSAALARATAKFLVVSFTSDWRFTPARSREIVKAL
                     LDNRLGVSYAEIDAPHGHDAFLMDDFRYHNLLRAYFDNISREVSA"
     gene            3849..4469
                     /gene="metW"
                     /locus_tag="VN496_02215"
     CDS             3849..4469
                     /gene="metW"
                     /locus_tag="VN496_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HXF15785.1"
                     /translation="MIQNAPVFLSARPDFVAIAKWIQPNAKVLDLGCGDGSLLHHLQE
                     ARGVTGYGVEIDDAKVLACVKSGVNVLQNDFESGLSGFEDGSFDYVILSQTLQAIRHT
                     ERVVNEMLRVGRQGIVTFPNFGYWRPRLQVLAGNMPVSKALPYEWYDTPNIHLFTIDD
                     FEKFCAAQGIRILERNVMVDGRPVAWMPNLRGSLAVYRFDRAVRVG"
     gene            4479..>5164
                     /locus_tag="VN496_02220"
     CDS             4479..>5164
                     /locus_tag="VN496_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF15786.1"
                     /translation="MTQASLVSALFTRRMLICVFTGFSSGLPLYLLLNLLPAWLRTEG
                     INLKVIGAFALIQFPYTWKFLWSPVLDRYVIRPLGRRRGWMLVTQIPLMASVAWLGTL
                     SPHTELSWVIYAATAVAFLSATQDIAIDAYRRELLSDKELGLGNAVHVNAYRIAGLIP
                     GSLSLILADHFPWEMVFGITALFMLPGIAMTLLVAEPEMAAPPKTLRAAVVEPFREFV
                     GRAGVRSALLV"
BASE COUNT         1059 a         1414 c         1550 g         1141 t
ORIGIN      
        1 gaagaccggt atcgtgaact gggatcccgt ggaccagaca gtccttgcca atgagcaggt
       61 gatcgaaggt cgcggttggc gaaccggcgc gctggtggag aaacgcgaga tcccgatgta
      121 ttacctcgcg atcacgaagt acgcggagca gttgctgtct tcactggatt cgctgccggg
      181 ctggccggag cgcgtgaaga ccatgcaggc gaactggatc ggaaaaagct atggcgtgcg
      241 cgcggcgttc ccgtacgtct tgaatggcaa gcccgaaaaa ctttgggtat tcacgacacg
      301 cgccgacacc atcatggggg tgacattttg tgctgtggcc gccgaacatc cgcttgccgc
      361 ggccgcggcg cagcgaaatt cggggcttgc cgagtttgtc gaagagtgca ggcgcggcag
      421 catcatggag gccgaccttg caaccatgga aaagaaaggc atgcgcacgg ggttgttcgt
      481 caatcatccg ctcacgggca agaagatcga agtgtgggtt ggaaattatg tgctgatgag
      541 ctatggcgaa ggcgcagtca tgggtgtgcc ggggcacgac gaaagggact tcgcgtttgc
      601 caagaaatac ggcttgccga tcctgcaggt catcgacgtg cccggaaaac ggtatgacgt
      661 tgatgcgtgg caaccctggt acgcggatca gggcctatgc atcaattccg gtaaatacga
      721 cgggctggat tttgtgcgct cggtagatgc catcgcggcg gatctcaacg ccgaaggctg
      781 tggcgaaaag caggtaacgt ggcgtctgcg tgattggggc atttcgcgcc agcgctactg
      841 gggctgtcca atcccaatcg tgcattgcgc cgcttgcggc gacgttccgg ttccggaaga
      901 ccagcttcct gtcctgttgc ccgaggactg tgtaccggac ggcagtggca atccactgaa
      961 caagcgtgct gatttcgtca acacggcctg tccgaagtgc gccaggccgg cgaagcgcga
     1021 gaccgacacc atggacacgt tcgttgattc atcctggtat ttcctgcgct tcgcctgccc
     1081 tgatcaacat agcaagatgg tcgacgaacg cgtcaactac tggttgccgg tggatcagta
     1141 tatcggcggc atcgagcacg cgattctgca tctgctttat tcccgcttct ggaccaaggt
     1201 catgcgtgac ctgggactgg tgaagttcga tgaaccgttt acgcacttgc tgacccaggg
     1261 aatggtgctg aacgaaattt tccagcgcaa ggtggatggt ggccgtgtcc agtatttcaa
     1321 tccatcggat gttgaaattg aattcgacga ccagggcaaa cgcagccgca cgattctgaa
     1381 gtccgacggc cgaccggtcg agtccggtgg aatacgcacg atgtccaagt ccaaaaataa
     1441 cggggtggat ccccaagcct tgattgacca gtatggcgcg gatactgcgc gctttttcat
     1501 gatgttcacc agtccgcctg aagacaccct tgcgtggaat gacgacggcg tggagggggc
     1561 gtaccggtat ctgcgccgtc tatggaaatt taattacgaa catcacacgg caatatccga
     1621 tagtgcggcc agcgttacgg cggcggaccg gaattacagg cgagaactgc atctgctgct
     1681 gcagcaggcc aatttcgatt acgagcgcaa acagttcaat accgtagctt cggcgtcgat
     1741 gaagatcctc aatctgctgg aacaggcggg tgctggaaaa gccggcttgt tgcgcgaagg
     1801 aatgagtatc ttgctccggc tgttgtcgcc catcgcgccg catatcgcgc aaacgttatg
     1861 gcgcgaatgc cgttttgggg atgatattct gaatgcagac tggccggtag tcgacacagc
     1921 ggcattggtg cgcgaacaga ttgaactggt gctgcaggtc aacggcaaac accgaggctc
     1981 tgtcgttgtt ccgagcgacg cgtcccgcgc ggccatcgag gacctggcac tcgcaagtcc
     2041 gaacgtccag aaacatattg caggcctgcc ggtcaaaaaa gtcatcgttg taccggggcg
     2101 gctggtcaat atcgttgtat gaacagacaa tgaacagcgt gtgtaaacgt tattcgctcc
     2161 gggatcctgg cctatcggct ttgaccactg cgattatcgc cgttgcgatg atattgtctt
     2221 cctgcggctt tcagttgcgg ggtcaagcag ccattccgta taaaacgctg ttcatcgaga
     2281 cttctggtta ttcccagttt gcgaacgatc tcgagagggc aattcgcttc agtagcggaa
     2341 ccaaggtagt cagcaaccgc gatgatgcgc aggcgatcct gcgaatcatg ggagaagcgc
     2401 aggaaaagca cattctttcg ctgtcttctg gcgggcgggt acgcgaattt gaattgaact
     2461 accgggtcaa ttatcgattg atcgaccatg cgggcaagga tttggcacaa cccgggcaga
     2521 tcgacctgcg tcgcgacatg acttacgacg acaccgaagt ccttgccaag gaatcggaag
     2581 agcagctgct gtaccgggac atgaagaagg atgccgtaca gcagatgatg cgtcgcctgt
     2641 cggcagccaa gccggtcgca tagattctga tgcggacgat ataatccgcg caacgacaat
     2701 cccggatttg cgatgccacc cgaaggttca gtcggcgtag tcagcccgag gctcgcccat
     2761 ttcaatgatc ccttggcgct ccggagcggc gcccggctgg aagcttacga tctcgcgtac
     2821 gagacgtacg gtacgctcaa ttcaaaacgc agcaacgcgg tgctggtttg tcatgcgctc
     2881 aatgcatcgc atcatgttgc tggaagctat gccgacgatc cgggcaacgt gggttggtgg
     2941 gataacctca ttggcccagg gaagccggtc gataccgacc gcttcttcgt ggtcggcgtc
     3001 aataacatcg gcggttgtca tggttccacg ggaccggcca gcgtcaatgc ttccaccggc
     3061 caaccctggg gagctgcctt tccggtcgtc acggtggagg actgggtgga agcacaggcg
     3121 cggctcgcga cgaggctcgg catcgaccga ttcgccgcgg tgatgggcgg gagtcttggc
     3181 gcaatgcagg ccatgcaatg ggccattagc caatcttctc gcgtggggca ctccatcgtc
     3241 atagcggccg caccaaagct ctcggcacag aacatcgcat tcaacgaagt agcccgccag
     3301 gccatcacca cggacccgga ttttcacggc ggcaattatt attcccatgg tgtcgtgccg
     3361 aaacgcggtt tgcgtattgc gcgcatgatc ggccatatca cctatttgtc cgacgatgca
     3421 atggcagaga aatttggccg cgagcgccgg gccggtgctt tgcaattcaa tttcgacgtt
     3481 gagttccaga tcgaatctta tttgcggtat caggccgaca agttcgccga gcgcttcgat
     3541 gccaatacct atttgcgcat caccaaggca ctcgattatt tcgatcccgc cgctgcgcat
     3601 ggcggcattc tatccgccgc tcttgcgcgt gcgacggcaa agttcctggt ggtctcattc
     3661 acttccgatt ggcggttcac accagcacgt tcgcgggaaa tcgtcaaggc tttgctggac
     3721 aaccggctcg gtgtcagcta tgcggagatc gatgcgccac atggccacga tgcctttctg
     3781 atggacgatt ttcgctatca caatctgtta cgcgcatact tcgacaacat atcgcgcgag
     3841 gtgtccgcgt gatacagaat gcgccggtct ttctttccgc ccgcccggat ttcgtggcga
     3901 tcgcaaaatg gatccagccc aatgcaaaag tgctggattt gggctgcggg gatgggtctt
     3961 tgctgcatca ccttcaggaa gcgcgcggcg tgacgggtta cggagtggaa atcgacgacg
     4021 ccaaagtcct tgcctgcgta aaaagcggtg tgaatgttct gcaaaatgat ttcgaaagtg
     4081 gcctttccgg attcgaggat ggttcgttcg attacgtgat cctgtcacag acactgcagg
     4141 ctattcgtca taccgaacgt gtcgtcaatg aaatgttgcg ggtcggccgg caaggcatcg
     4201 tcacattccc taattttggc tactggcgcc cccgccttca ggttctcgcc ggcaacatgc
     4261 cggtgtccaa ggcattgccg tacgagtggt atgacacgcc gaacattcac ctctttacga
     4321 tcgacgactt cgagaagttc tgcgctgcac agggcattcg catcctggag cgaaacgtga
     4381 tggttgatgg ccgcccggtg gcatggatgc caaacctacg gggctcgctg gcggtctacc
     4441 ggtttgatag agcggtccgg gtcggctgat tgcgcggcat gacacaggcc tcgctggtct
     4501 cggcgctgtt cacgcgccgc atgctgatct gcgtattcac cggttttagc tccggtctgc
     4561 cgctttactt gctgctcaat ctgctgcctg cgtggctgcg taccgaaggc atcaatctca
     4621 aagtcatcgg cgcattcgcg ctgatccagt ttccctacac ctggaaattt ctctggtcac
     4681 ctgttctcga tcgctacgtg atacgcccgt tgggccggcg ccgcggctgg atgctggtga
     4741 cgcaaattcc gctcatggcg agcgttgcat ggctgggtac gctgtcaccg cataccgaac
     4801 tgtcctgggt catttacgcc gcaaccgcag tggctttttt gagtgcaaca caagatatcg
     4861 cgatcgatgc ctatcgccgc gagctgctgt ccgataagga actcggtctt ggcaatgccg
     4921 tgcacgtaaa cgcttatcga atcgccggac tgatccctgg gtcgctgtcg ctcattttgg
     4981 cagaccactt tccctgggaa atggtgttcg gcatcaccgc gttgttcatg cttccgggca
     5041 tagcgatgac cttgctggtc gccgaaccgg aaatggcggc cccgccgaaa acgctgcgtg
     5101 cggcggtggt cgagccgttt cgcgagttcg tgggccgggc cggtgtgcgc agtgcgctgc
     5161 tggt
//
LOCUS       DATMZQ010000067         5530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1774854, whole genome shotgun sequence.
ACCESSION   DATMZQ010000067 DATMZQ010000000
VERSION     DATMZQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774854"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1465)
                     /gene="larA"
                     /locus_tag="VN496_02225"
     CDS             complement(182..1465)
                     /gene="larA"
                     /locus_tag="VN496_02225"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HXF15787.1"
                     /translation="MQLDLNYGRGRYPLELPDHWDVTVIRKPAMPLEKDPRSAVSSAL
                     ARPVAANSLVQEARGLKSACILICDITRPVPNGLLLPEIIRELLNAGMNADQITVLVA
                     TGLHRPNEGAELEELVGDAWVLKTVRVVNHFARDDAAHVDLGRTPRGTPVKLDRRFVE
                     ADLKIATGLVEPHFMAGYSGGRKVIAPGVAHRDTITTFHSARFMAHPMADNCVLDGNP
                     LHEEQLEIVRMLGHVLAVNTVIDEHRRLSFVNYGEILASHLQAVDFTQRYARVPVGRR
                     FKTVVTSAAGYPLDKTYYQTVKGMVGPVDILEPGGNLVIVSECSEGLGSEEYADAQRR
                     LIGLGAEGFFADISRKQFADIDEWQTQMQLKPMKAGVVHLYSGGLPQPDRALTGVTLI
                     DSVEETLQECMQRNGNHPLAVIPEGPYVVPYFEAA"
     gene            1569..2288
                     /locus_tag="VN496_02230"
     CDS             1569..2288
                     /locus_tag="VN496_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15788.1"
                     /translation="MNLSDPVMAALIGASATFTTALFQLLINARRQAAERAAGKPASR
                     KSGTWLAIFALMLAAAVGGYAFSEYQGFRDRDDEKRLRQEMQTSLRDIGNVAVRLERA
                     GLQRSDQGDVDAMLAAERRRGIEGVAAVIEVPACAGSQSGTDASAMCNEAHAVHVTVC
                     AVIPLTAVVSEVQVFTRAADAAQAWTDAKVQPGQDAGSAKFIDTFSERAQGDAKEVCQ
                     QFMHWNSQKPRLARILVKYTI"
     gene            2342..3508
                     /locus_tag="VN496_02235"
     CDS             2342..3508
                     /locus_tag="VN496_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXF15789.1"
                     /translation="MSRINRVEVQEFAFELPDLGVDSGGFNIVYYPGNRLNLTKYAVA
                     IEADDGARGEYVTVWGGTKMALGQTLALAPQLLGRDPHQRECIYDDFKRALRQYDHMG
                     VGCLDIALWDLAGKQFGVPIWKMLGGWRQRLPTYASSYHGDRNGGLSSAQAYVDFAEQ
                     CFELGYRAFKMHGWCDGNAKEESEAVLALGRHFRGRMDLMLDPACELRTFADALAVGR
                     ACDEAGFFWYEDPMRDGGWSQHLHRKLRHFIKTPLLQTEHVRGLEPKADFIVNDATDF
                     VRADPEYDLGITGTMKIAHLAEAFGLDVEIHAPGPAHRHCMAAIRNTNYYELALVGPK
                     CANAMPPVYADGYSDQLDAVGGDGCFPVPNGPGLGVEYDWDFIRQHSVMLHEFK"
     gene            3773..5446
                     /locus_tag="VN496_02240"
     CDS             3773..5446
                     /locus_tag="VN496_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HXF15790.1"
                     /translation="MIWSQVYDPLGNTILSTFVCAIPVLVLLGGLGIFRIRAHYAALA
                     GLVAAVIIAMFIIGMPGEMTLRAGFYGALFAATTVCWIIFNLIFLYRMTLQVGLFKIL
                     QDSIGGITNDRRLQLILIAFCFGAFFEGAAGGGTPVAVTGAILIGLGFNPLAASGLSL
                     IANTAPVAYGGLGTPIIVLHSVTQPENPDYLFALSSMVGRQLPFFSVIVPIWLVLTFC
                     GWRRTVEVWPAVLVAGLSFAIPQFVISNYHGPWLVDMLGAMISMITLAVFLAIWKPKS
                     VMLDASGAGVTVPRAQAAAIHHGHSRDLVIKAWTPWVILTVVLFLWGLPLVKAFLNLK
                     GITYWEWQIPGLHNLVQRVEPAVAAPRVEPAIWKWGIISATGTGILIAAIIAGFVMGL
                     KVKDMFKTWIETFGVMKYSLLTILAMLALGYITRYGGMDGTLGLAFANTGWLYPFFGT
                     MIGWIGVALTGTDAGSNALFGSQQVITANKLGLSPTLMAAANSSGGVMGKMIDAQSIV
                     VASVATNQQGREGVILRYVFWHSISLAALVGMLIMLQAYVPFFQAMVPQ"
BASE COUNT         1072 a         1587 c         1663 g         1208 t
ORIGIN      
        1 cccggccggc cctgatttgc gagcgcactt ccggacgctg gaattcccgg gcgatcgttg
       61 ccggaatgtc cccgaggtgt gccgagtcga atttctggcg tacattcacc atgcgcggga
      121 gcccaacatc caatccgccg gcaatgttta cctctatttt gtcttcaatc atgttggctc
      181 ctcaggcggc ttcaaagtac ggcacgacat acggcccttc gggaatcacc gcgagcgggt
      241 gattcccgtt gcgttgcatg cactcttgca atgtttcttc gactgaatcg atcagggtga
      301 ccccggtgag tgcgcgatcg ggttgcggca agccgccgct atacaagtga accaccccgg
      361 ctttcatggg cttcagttgc atctgggtct gccactcgtc aatgtcggca aactgcttgc
      421 ggctgatatc ggcgaagaac ccttctgccc cgagcccgat caggcgcctt tgcgcatccg
      481 catattcttc cgagccgagt ccctccgaac attcggatac gatcacgaga ttgccgccgg
      541 gctcaaggat atcgaccggt cccaccattc ctttcacggt ctggtaatac gtcttgtcga
      601 gcggatagcc ggcagcgctg gtcacgacgg tcttgaagcg gcgcccaacc ggaacgcgtg
      661 catagcgctg cgtgaagtct accgcttgca gatggctggc gagaatttcc ccatagttca
      721 cgaacgacag ccgccgatgc tcgtcgatga ccgtgttcac tgcaagcaca tgacccagca
      781 tgcgcacgat ttcgagctgt tcttcgtgta atggattacc gtccagaaca cagttgtctg
      841 ccatggggtg agccatgaag cgcgcactgt ggaatgtggt aatggtatcg cggtgcgcca
      901 cacccggcgc aatgaccttg cgtcccccgg agtaccccgc catgaaatgc ggttccacga
      961 ggcccgttgc aattttcagg tcggcttcga caaaacggcg atcaagcttg actggcgtac
     1021 ccctcggggt tctgccgaga tccacatgag cggcatcatc gcgcgcaaaa tgattgacga
     1081 cccgcacggt tttcagtacc caggcatcgc ctaccagttc ctcgagttcg gcgccttcgt
     1141 ttggacggtg caggccggtg gcgaccagta ccgtaatttg atcggcgttc atgcccgcat
     1201 tgagcagctc acgaatgatc tcgggcaaaa gcaggccatt tggcaccggg cgcgtaatgt
     1261 cgcaaatgag gatgcacgcg gatttcagtc cacgcgcctc ctgtactagc gaatttgccg
     1321 caaccggccg cgcgagcgca ctgctcaccg ccgacctggg atccttctcc agcggcatcg
     1381 cgggtttgcg gatcacggtg acgtcccagt ggtcgggcaa ttcaagggga taacggccgc
     1441 gaccgtaatt tagatcaagt tgcatcggca ttcggagaaa aaaagcaagg aaactatagc
     1501 actggcgggg cgctcgcgtg cgcacacagg cttccgaacg ttcgttctag aaggtagcat
     1561 ctcataacat gaatttatcc gaccccgtaa tggcggcatt gatcggagcg agcgctactt
     1621 ttacgaccgc gctgttccag ctactgatca atgcccggcg tcaggctgcc gaaagagcgg
     1681 caggcaagcc agcttcgcgc aaatccggta cgtggctggc gatctttgcg ctgatgctgg
     1741 ctgccgcggt gggtggctat gctttttcgg agtaccaggg cttccgcgac cgcgatgacg
     1801 aaaagcgttt gcgccaggaa atgcaaacca gtctgcgcga catcggaaac gttgcggtac
     1861 ggcttgaacg cgcgggcctg cagagaagtg accagggcga cgtcgatgca atgctggccg
     1921 ccgaacgccg aaggggcata gaaggcgttg ccgcggtaat cgaggtcccg gcctgcgcag
     1981 gctcgcaatc cggcacggac gcctccgcca tgtgcaacga ggcacatgcg gttcatgtta
     2041 ccgtttgtgc agttatcccg ctaacagccg tggtaagcga agttcaggtt ttcacacgag
     2101 ccgcggatgc cgcacaagcc tggaccgacg ccaaagtaca gccaggacaa gacgcaggct
     2161 ccgcgaaatt catcgacacc ttttcggaac gtgcacaagg cgacgcgaag gaggtgtgcc
     2221 agcagttcat gcattggaac agccagaagc cgcggctggc gcgcatactg gttaaatata
     2281 ccatctgatt ctcgtcggct tgttgttgag caaaatcgcc gcatgcacgt tgggagctgc
     2341 aatgagccgt atcaatcgcg tggaagtgca ggaattcgcc tttgaattgc ccgacctcgg
     2401 tgtcgatagc ggcggcttca atatcgtgta ctaccccggc aatcggctga acctgaccaa
     2461 gtatgcggta gcgattgaag ccgatgacgg cgcgagaggt gagtatgtga ccgtgtgggg
     2521 cggaaccaaa atggcgctgg gccagacgct ggcgcttgcg ccccaattgc ttggccgcga
     2581 tccgcatcag cgtgaatgca tttatgacga tttcaagcgg gcattgcgcc aatacgacca
     2641 catgggcgtt ggttgcctgg acatcgcgct ttgggatctc gctggaaaac agtttggcgt
     2701 gccgatctgg aaaatgctgg gcggatggag gcagcgtctg ccgacgtatg ccagttccta
     2761 tcacggcgac cgcaacggcg gcttgtcgag cgcccaggct tatgtcgatt tcgccgaaca
     2821 gtgcttcgaa ctcggctatc gcgcgttcaa gatgcacggc tggtgtgacg ggaacgcaaa
     2881 ggaggaaagc gaagccgtgc ttgctttggg gcgccatttc cgtggacgca tggatctgat
     2941 gctcgacccg gcttgcgaat tgcgcacctt cgcagatgcg ctggctgttg ggcgcgcatg
     3001 cgatgaagcg ggattttttt ggtacgaaga cccaatgcgc gacggcggct ggtcgcagca
     3061 tctgcatcgc aaactccgtc acttcatcaa aaccccgctg ttgcagactg aacatgtgcg
     3121 cggactcgaa ccgaaagcgg acttcatcgt caatgacgcg acggatttcg tgcgcgcgga
     3181 tcccgaatac gaccttggca ttaccggcac catgaaaata gcgcatctcg ccgaagcgtt
     3241 cggcctcgat gtggagattc atgcgcctgg tccggctcac cggcattgca tggcggcgat
     3301 tcgcaacacg aattattacg agctggcctt ggtcggtcca aagtgcgcca atgcaatgcc
     3361 gcccgtctat gcggatggat acagcgatca actcgatgca gtcgggggag acggctgctt
     3421 tcctgttcct aacgggccgg gcctcggtgt cgagtacgac tgggatttca tcaggcaaca
     3481 tagtgtgatg ctgcatgagt tcaagtaagt ccggcgaggc gcgctggagc atttggctct
     3541 gccagtggag ctacgcgatg accgctacgc gtgcgtagcg ttctcagcgg cagcaaaggc
     3601 tcccgcctgg cttccgtctc ttcctgcggc gcagcatcgg ttcgtccata cttgcgcatg
     3661 cgaaccagcg agttagccgg acaattcgac tatacttccc ccgcgattgg gaagacgcgg
     3721 ccgcctggcc gcgttacccg atcaacataa aaaaattatc aggggagcct tcatgatctg
     3781 gtctcaggtt tacgacccgc tcggcaacac catcctgtcc acgtttgtct gcgcgattcc
     3841 ggttcttgtc ttgcttggcg gattggggat ttttcgcatc cgcgcccact atgcggcgct
     3901 cgcgggcctg gttgcggctg tcattatcgc gatgttcatc atcggcatgc cgggagagat
     3961 gacgctgcgc gccggttttt atggcgcgct gttcgccgcc acgacggtct gctggatcat
     4021 cttcaacctg attttcctgt accgcatgac cctgcaagtg gggttgttca agatcttgca
     4081 ggacagcatc ggcggcatta ccaacgaccg cagactgcag ctgatcctga tcgcattctg
     4141 cttcggggcg tttttcgaag gcgctgccgg cggcgggacg ccggttgcag taaccggggc
     4201 gattctgatc gggcttggat tcaatccgct ggcggcatcg ggcctgtcgc taatcgccaa
     4261 tactgcgcca gtagcctatg gcggcctcgg aacgccaatc atcgttttgc actcggtcac
     4321 tcagccggaa aatccggact atctctttgc gctatcctcg atggtaggac ggcaactgcc
     4381 cttcttttcg gtgatcgtcc cgatttggct ggtattgacg ttttgcggat ggcgccggac
     4441 ggtggaagtt tggcctgcgg ttctcgtggc cggcctgtca ttcgcgattc cgcaattcgt
     4501 gatctcgaat tatcacggtc cgtggttggt ggacatgctc ggcgccatga tctcgatgat
     4561 cacactggcc gtcttcctgg ccatctggaa accgaaatcg gtcatgctcg atgcgtctgg
     4621 tgccggcgtc accgtgccgc gcgcccaggc ggccgctatt catcacgggc acagccgcga
     4681 tcttgtgatc aaggcctgga cgccgtgggt cattttgacc gttgtgttgt tcctgtgggg
     4741 cctgccctta gtcaaggcgt tcctgaatct gaaaggcatc acctactggg agtggcaaat
     4801 cccgggactg cacaaccttg tgcagcgcgt ggagccagcc gtcgctgcgc cgagagtcga
     4861 gcccgccatc tggaagtggg gcatcatttc cgctacgggg accggcatcc tgatcgccgc
     4921 gatcattgcg ggcttcgtga tggggctcaa ggtcaaggat atgttcaaga cctggattga
     4981 gaccttcggc gttatgaagt actcgttgct gaccatcctg gccatgcttg cgcttgggta
     5041 tatcacgcgg tacggcggca tggatggcac cctgggcctg gctttcgcca acaccggttg
     5101 gctgtatcca tttttcggca cgatgatcgg atggatcgga gtcgcgctga ccggaacgga
     5161 tgcaggctcg aacgcgctgt ttggcagcca gcaggtcatt accgcgaata aactgggcct
     5221 cagcccgacg ctgatggcgg cagcaaacag ttccggcggc gtgatgggca aaatgatcga
     5281 tgcgcagagc atcgttgtcg catctgtggc taccaaccag cagggcaggg agggcgtaat
     5341 cctgcgctac gtgttctggc acagcatatc gctggctgcg ctggttggca tgctgatcat
     5401 gttgcaggcc tacgtgccat tcttccaggc catggtcccg caatagaaag caccctcttc
     5461 cgacggcacg gcgacgtttt gccgtgacat ttcatttgac ggacatgcga aatctgcagg
     5521 tggtactggc
//
LOCUS       DATMZQ010000068         8333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_50834, whole genome shotgun sequence.
ACCESSION   DATMZQ010000068 DATMZQ010000000
VERSION     DATMZQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50834"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VN496_02245"
     CDS             complement(<1..216)
                     /locus_tag="VN496_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXF15791.1"
                     /translation="MRDAQRKRILVTGGAGFLGSHLCERLLQHGHDVLCVDNFYTGSK
                     DNIVQLHDNAYFELLRHDITFPLYVEVD"
     gene            complement(213..1352)
                     /locus_tag="VN496_02250"
     CDS             complement(213..1352)
                     /locus_tag="VN496_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXF15792.1"
                     /translation="MPDKLGKIGSVAVLRALQLGDMLCVVPALRALRHAFQDARITLI
                     SLPWARDFRNRFSQYVDDFIEFPGYPGLPERAFDAAAVTAFLQQAQRHSFDLALQMHG
                     HGRIANPLVQLIGASRSAGYYLPGEYCPDEERFLPWQAREHEILRWLRLVKALGVPAQ
                     NAELEFPLLPADYAEFENISDQHSLDALSYVCLHPGSQLPSRRWPAERFAKVGDELSA
                     LGYRVVLTGTAAERQLTASVARAMRIAPVDLAGRTSLGALAALIRNAAGLVCNDTGVS
                     HIAAAVQTCSVIVSCGSDPARWAPLDTRLHRMVFAPVNCRPCSYRDCPFDHPCARAVS
                     ALEVAGAMAALLRRHEQPRHRRPHAPGRTETFARPATNHEQRRSA"
     gene            complement(1345..3138)
                     /locus_tag="VN496_02255"
     CDS             complement(1345..3138)
                     /locus_tag="VN496_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXF15793.1"
                     /translation="MYTLGINAAFHDTAACLVKDGQVVSAAEEERFTRIKHGKRPLPF
                     TTWELPFNAIDYCLGHSGIELADIDHVAYSYDPYLLLDKDSAQNASIALPLEPSKQKV
                     AVNWVSPWEPLALAYVINAERQLIDGVPHHLRGRYRTAKQARRWRWHFVEHHLAHEAS
                     AFLAAPFDSCAVLTLDGRGERATTSYGQFSGRGFRRLLQIDVPDSLGLLYEAVTSYLG
                     FLHSSDEYKVMALASYGSPHYLDEFRDIVRLEGEGRYTTRNLRLEERFGPARQRNAEL
                     LQRHFDIARSLQEVLEERVIELARWLRAETEENAVAMAGGVALNCVMNGRLRDAGIFD
                     QVWVQPAAGDAGTALGAALWIDWKTRNAGTQKMLPGYRMDHAFLGPEYSAEEIETFLK
                     RAKLPYRRLANVAEETAELLAQDKIVGWFQGRMEFGPRALGARSILASPLSPDMQQRL
                     NEIKDREDFRPVAPAVLEEEAARWFQGARVSPFMLFIFDVAEDKGERIPAVRHVDGTA
                     RVQTVNRRQHPLYYDLLKAFQQITGVPVLVNTSFNTRSEPIVCSPRDAIECFWTSPLD
                     ALVIGPFMLEKSSGTAQTTTRTARQAEPWHA"
     gene            complement(3141..4280)
                     /locus_tag="VN496_02260"
     CDS             complement(3141..4280)
                     /locus_tag="VN496_02260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXF15794.1"
                     /translation="MNWEQARNVLCVRLDSLGDVLMTTPAIRALRESVAGRRITLLTS
                     PSGAKAARLVPEIDAVVEFRAPWMKPATEGPAADAVMKRRLAHGKYDAAVIFSVYSQN
                     PLPAAYLCYLAGIPLRLAHCHENPYHLLTHWVADPEPANVSRHEVRRQLDLVESVGSR
                     TNNERLSMAVPKPAKARARRLLHALGITLRKPLVLVHPGATAASRRYPPAQFAQAMSI
                     LGRRIDCEIVFTGGAEEKPLIEGIRLGMDVPSHSAAGLMELGELGAAIECADVMVSNN
                     TGPVHMAAALGTPVVDLYALTNPQHTPWQVANKVLFHDVACRNCYKSSCPLGHHDCLR
                     RVQPETVAAAALELLGSRPSLRFRATGRSRVAGLIPAFNTIAAGQ"
     gene            complement(4277..4885)
                     /locus_tag="VN496_02265"
     CDS             complement(4277..4885)
                     /locus_tag="VN496_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXF15795.1"
                     /translation="MAIKARTSAVFLDKDGTLIEDVPYNVAPEQMRLAPGAGEALHLL
                     ARSGYRLIVVSNQPGVALGLFPEYALQAVEQRLQELLLPAGVSLHGFYYCPHFPRAKI
                     PAYRLQCNCRKPAPGMLTRAAKDHGLQLHRSWLVGDILDDIEAGRRVNCKTVLINNGN
                     ETEWELTAERCPHVVATNLLEAATTIVRVDRAIAAETAGVSA"
     gene            complement(4876..5655)
                     /locus_tag="VN496_02270"
     CDS             complement(4876..5655)
                     /locus_tag="VN496_02270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15796.1"
                     /translation="MKTESLPPANLDVHHAAQLAPLRDKIAFVTGGGRGLGAAICNAL
                     AAAGATVVVADVNLENARAVARNAGQRGGRAHALKVDIGADEQVQKAIADTIASHGRL
                     DALINNAGIDFTLPIEELSVDQWDAVVRTNLRGPFLLCKYAVPVMRRQGGGHIVNIAS
                     TAARRAWPNASAYHASKWALLGLSHALHAELRPHGIKVSAVIAGGMRTPFLLDRFPDL
                     DPDVLQDPANVAATVCFLLTQPAETVIPEVMVLPMRETSWP"
     gene            complement(5666..6931)
                     /locus_tag="VN496_02275"
     CDS             complement(5666..6931)
                     /locus_tag="VN496_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXF15797.1"
                     /translation="MLRRIALISDHASPLAAAGGVDSGGQNIYVAQVARHLVSLGYRV
                     DVFTRRDNPLLAEVVPCGDGLRVVHVPAGPPQFVRKEDLLPTMQQFAAYVATFCTREG
                     HYDLVHANFFMSGLVAQILKESLGIPFVITFHALGRVRRLHQREADQFPAERIDIEDR
                     IVENADGIIAECPQDEADLLSLYKADPARISVIPCGFDAAEFWPIAKRFARKTLGLAP
                     NQPLLLNLGRTVPRKGIDNAILALTRLAGKDAIRPTLMVVGGNSDLPDPALTPEIARL
                     RSIAQAENVSEQVMFVGRRSRELLKLYYSAADIFITTPWYEPFGITPLEAMACGTPVI
                     GSDVGGIKYSVVDGETGCLVPPNDPDALAQCIQGLYQQPDRLKEFGRNGIRRVNRCFT
                     WGGVTGSIASFYKQVCGESIEPQRIAALA"
     gene            7431..8231
                     /locus_tag="VN496_02280"
     CDS             7431..8231
                     /locus_tag="VN496_02280"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXF15798.1"
                     /translation="MQGKRIAVLESRFGEGLVTLLEKRGAIAFHAPALAEEPDIDSKM
                     IGRLLEEWAASPFVLAIFQTGVGTRALFEAADRLGMAESLLALLATCKVAVRGPKPTA
                     ALRARHVRIDLMALEPYTTAQVLESVRGFDLTGARVLVQRYGETNLGLQAALEARRAQ
                     VVEVPTYRWALPADTRPLERLIDGLAQGQLDAAIFTSASQVRNLFAIAAQRDISALLR
                     QSLNTVLVASIGPVCSHALRAFGVRVSLEATPPKMGPLVEMLTTAWPI"
BASE COUNT         1685 a         2483 c         2514 g         1651 t
ORIGIN      
        1 atcgacctcg acgtaaagcg gaaaagtaat gtcgtggcgc agcagttcaa aataggcgtt
       61 gtcgtgcaat tgcacgatgt tgtccttgct gccggtatag aaattgtcca cgcacaggac
      121 gtcgtgaccg tgctggagca gtcgctcaca gagatgcgaa cccagaaatc ccgctccacc
      181 ggttaccagg atgcgttttc gttgcgcgtc ccttatgcag acctccgctg ttcatggtta
      241 gtagctggac gcgcgaacgt ctcggtgcgc ccgggggcat gcggcctgcg atggcggggt
      301 tgctcgtgcc gacgcagcaa cgccgccatg gcgccggcga cttcgagtgc tgaaacggcg
      361 cgggcgcaag ggtggtcgaa tgggcagtcc cgataggaac acggccggca gttcacaggc
      421 gcaaacacca ttcgatgcaa tcgagtatcg agcggcgccc agcgcgccgg atctgacccg
      481 caacttacga tgacgctgca ggtctgcacg gcggccgcaa tatgagatac gccggtatcg
      541 ttgcacacga gtccggcagc gttccgaatc agcgcagcca gcgcgccaag cgatgttcgg
      601 ccggcaaggt caaccggcgc gattcgcatg gcgcgcgcaa cgctggcagt gagttggcgc
      661 tccgccgccg taccggtgag cacgacccga taacccaagg cggacagttc atcgccaacc
      721 tttgcaaagc gctccgcggg ccagcggcgt gaaggcagct gcgatcccgg gtgcaggcaa
      781 acgtacgaca gggcgtccaa agaatgctga tcagaaatgt tttcgaattc tgcgtagtcc
      841 gcaggcagca gcggaaattc gagttcggca ttttgcgccg gcacgcccag cgcttttacc
      901 aggcgcaacc aacgcaaaat ttcatgctcg cgagcttgcc agggaagaaa gcgttcctca
      961 tccggacaat attcaccggg caggtaatag cctgcgctgc ggcttgcgcc gatcagctgg
     1021 accagcggat tggcgatgcg cccatgaccg tgcatttgca atgccagatc gaagctatgc
     1081 cgttgcgcct gctgcaggaa agccgtaaca gctgcggcgt caaaagcgcg ctccggcaat
     1141 cccggataac cgggaaactc gatgaagtcg tccacatact gactgaagcg gttcctgaaa
     1201 tcgcgcgccc agggcaggct aatcaaggtg atgcgcgcgt cctggaacgc gtgccgcagt
     1261 gcgcgcaatg ccggaacaac acacagcatg tcgcccaact gcagcgcacg cagaaccgca
     1321 acggatccta ttttgccgag cttgtcaggc atgccagggc tccgcttgcc tggcggtccg
     1381 cgttgttgtt tgcgcggtgc cggaagactt ctctagcatg aatggtccta tgactaatgc
     1441 atcgaggggc gaagtccaga aacactctat tgcatcgcgc ggactgcaaa cgatcggctc
     1501 gctgcgcgta ttaaacgagg tattgaccag taccggaacg ccggtgatct gctggaatgc
     1561 cttcagaaga tcatagtaaa gtggatgctg cctgcgattg acagtctgca cgcgggctgt
     1621 gccgtcgacg tggcgcaccg ccgggatgcg ctcaccctta tcctctgcca catcgaaaat
     1681 gaacagcatg aaaggagaga cgcgcgcgcc ctgaaaccac cgtgcggcct cttcttcgag
     1741 cactgccggc gcaaccggcc ggaaatcttc gcgatccttg atttcgttca aacgctgttg
     1801 catgtccggc gacaatggcg acgccagaat ggagcgtgcg ccgagcgcac gcggtccgaa
     1861 ttccatgcgg ccctgaaacc agccgacgat tttatcctgc gccagcagct cagccgtttc
     1921 ttcggcaacg tttgcgagcc tgcgataggg caatttcgcg cgtttcagga aggtctcgat
     1981 ttcctctgcg gaatattccg ggccgaggaa cgcatgatcc atgcgatacc cgggtaacat
     2041 cttttgtgtg ccggcattgc gcgttttcca gtcgatccac agtgccgcgc ccagcgccgt
     2101 tccggcgtcg cccgcggcag gttgcaccca tacctgatca aagatgcccg catcgcgcaa
     2161 tctcccgttc atcacgcaat tgagcgcgac gccaccggcc attgcgactg cgttttcttc
     2221 ggtctcggct ctgagccagc gcgccagctc gatgacccgc tcctccagaa cttcctgtag
     2281 cgagcgcgcg atgtcgaaat ggcgctgcaa gagttctgcg ttgcgctggc gcgcgggacc
     2341 aaagcgttct tcgaggcgca gatttcgcgt cgtgtagcgg ccttcgcctt ccaggcgaac
     2401 aatgtcgcga aactcatcca ggtagtgcgg gctgccgtaa gaggcgaggg ccatgacctt
     2461 gtattcatcg gacgaatgca ggaatcccag atagctggtg acggcttcat agagcaggcc
     2521 gagtgaatcg ggcacatcaa tctggagcag acggcgaaaa ccgcgcccgg agaattgtcc
     2581 gtagctggtt gtggcgcgtt caccgcgccc atccagcgtc agcacggcgc agctgtcgaa
     2641 cggggcagcc agaaatgcac tggcttcgtg cgcgagatga tgttcgacga aatgccatcg
     2701 ccagcggcgc gcctgctttg cagttcgata gcggccgcgc aaatgatgcg ggacgccgtc
     2761 gatgagctgg cgttcggcat tgatcacata ggccagcgcc aatggctccc aaggggaaac
     2821 ccagttcacc gcgactttct gtttgctggg ctctagcggc agcgcaatgc tggcattttg
     2881 cgcgctatct ttgtccagca acaggtaggg atcgtacgag taagcgacat ggtcaatgtc
     2941 agcgagttcg atccctgaat gccccaggca ataatcgatc gcgttgaaag ggagctccca
     3001 ggtagtgaag ggcaggggac gcttgccgtg cttgatgcgc gtgaagcgtt cctcttccgc
     3061 ggcggacacg acctgaccat ccttgaccag acaggccgcg gtgtcatgaa aagcggcgtt
     3121 gattccaagt gtgtacatgg ctattgtccg gcagcaatgg tgttgaaggc aggaatgagg
     3181 ccggcaactc tgctccgccc cgtggcgcgg aaacgcaaac tcggccgcga tcccagcaac
     3241 tccagcgcgg cggctgccac cgtttccggc tgaacgcgcc gcagacaatc atgatggccc
     3301 aggggacaac tgctcttgta gcagttgcgg caggcgacat catggaacag caccttgttc
     3361 gccacctgcc acggtgtgtg ctgcggattg gtaagcgcgt acaggtccac caccggtgtg
     3421 ccgagcgcag cggccatgtg caccggaccg gtattgttcg acaccatgac atcggcgcat
     3481 tcgatcgccg caccgagttc accgagctcc atgagtcccg ccgctgaatg agaaggcaca
     3541 tccatgccca gacggattcc ctcgatgagc ggcttttctt cggcgccgcc ggtgaacact
     3601 atttcgcaat cgatacgccg tcccaaaatg ctcattgcct gtgcgaactg cgccggcgga
     3661 tagcggcgag aagcagccgt cgcgcccgga tgcaccagga ccaacggctt gcgcaaggtt
     3721 attcccaagg catgcagcag acgacgtgca cgggcttttg cgggcttggg cacggccatt
     3781 gacagccgtt cattgttcgt gcgtgacccg actgattcta cgaggtccag ctgacgcctg
     3841 acctcatggc ggctcacatt ggccggttcc ggatctgcca cccagtgcgt gagcaagtga
     3901 tatggatttt cgtggcaatg tgccaggcgc aatgggatgc cggcaagata gcaaagatac
     3961 gctgcgggca acgggttctg gctgtagacg gaaaaaatca cagccgcatc gtactttccg
     4021 tgagcaagcc gtcgtttcat tactgcgtcc gccgccggcc cttcagtagc gggtttcatc
     4081 cacggtgcac ggaactcgac gacggcgtcg atctcgggaa ccaggcgtgc ggctttcgct
     4141 cccgagggcg aagtcagcag cgtgatgcgc cgtcccgcaa ccgactctct cagtgcccgg
     4201 atggcgggtg tggtcatcaa cacgtcgccg aggctatcca ggcgaacgca caacacattg
     4261 cgcgcctgtt cccagttcat gcgctgacac ccgcggtctc ggccgctatt gcccggtcta
     4321 ctcgcacaat ggtggtggca gcctccagca gattcgtggc caccacgtgc ggacagcgct
     4381 cggcagtcag ctcccactcg gtttcattgc cgttgttgat cagcaccgtc ttgcaattca
     4441 ccctgcgtcc cgcctcgatg tcgtccagga tgtcgccgac cagccagctg cgatgcaact
     4501 gcagcccgtg atcttttgcc gcgcgcgtca acatacccgg cgctggcttg cggcaattgc
     4561 attgcaggcg ataggccgga atttttgccc ttggaaaatg tgggcagtag taaaagccat
     4621 gcagcgatac gccggcagga agcagcaatt cctgaagccg ttgctccact gcctgcagtg
     4681 catattccgg aaataatccg agcgctacgc caggctggtt cgatactacg atgaggcggt
     4741 agccggaacg tgcaagcaga tgcaaggctt ccccggctcc gggagcaagg cgcatctgct
     4801 ccggtgcgac gttgtacggg acgtcttcga tcagagtgcc gtccttgtcc aggaataccg
     4861 cgctcgtgcg ggcttttatg gccatgatgt ctctcgcatg ggcagaacca tgacttctgg
     4921 aatgaccgtc tcggccggct gcgtcagcag gaagcaaact gtcgctgcga cattcgccgg
     4981 atcctgcaag acatcgggat cgaggtccgg aaagcgatcc agcagaaacg gcgtgcgcat
     5041 cccgccggcg atgacggcgc tcactttgat gccatgtgga cgcagctctg cgtgtagcgc
     5101 atggctcaaa ccgagcagtg cccacttgct ggcgtggtac gcggaggcat tgggccatgc
     5161 gcgccgcgct gcagttgaag cgatgttgac gatgtgcccg ccgccctggc gccgcatgac
     5221 aggcaccgcg tatttgcaca gcaggaacgg accacgcaga ttggtgcgca ccaccgcgtc
     5281 ccattggtcc acgctcaatt cttcgatcgg caacgtaaaa tcgatgccgg cgttgttgat
     5341 gagtgcgtca agacgaccat gggacgctat ggtgtccgca atggccttct gcacttgttc
     5401 gtctgcgccg atgtccacct tcagtgcatg agcccggccg ccgcgttgac cggcattgcg
     5461 cgcaacggcg cgggcattct ccagattgac atccgctacc acgaccgtcg cgcccgctgc
     5521 ggctaatgcg ttgcagatcg cggcgccaag accgcggccg ccgccggtga cgaaagcgat
     5581 tttgtcacgc agcggcgcta attgcgcggc gtgatgaacg tcgagattcg cgggcggcag
     5641 agattctgtc ttcatgcgat atccttcagg ctagcgcggc gatgcgttgc ggctcgatgc
     5701 tctcgccgca cacctgttta tagaatgaag cgatggatcc ggtgacgccg ccccacgtga
     5761 agcagcgatt cacgcggcgg atgccgttgc ggccgaattc cttgagccgg tccggctgct
     5821 ggtacagacc ctgaatgcat tgagccagtg catcgggatc attgggtggc acgagacaac
     5881 cggtctcgcc atctaccacg ctgtatttga tgccgccgac gtctgatccg atcacgggcg
     5941 taccacaggc catcgcttcc agcggcgtaa tgccgaaggg ttcgtaccac ggcgtcgtga
     6001 taaaaatgtc cgccgcgctg taatagagct tcaacagttc ccggctacgg cggccaacaa
     6061 acatgacttg ttcgctgaca ttctcggctt gcgcaatcga gcgcaagcgc gcgatctccg
     6121 gcgttagcgc cgggtcggga agatccgagt tgccgccgac caccatcagc gtcggacgta
     6181 tcgcatcctt gccggcaagc cgtgtcagcg cgagaatggc gttgtcgatc cctttgcgcg
     6241 ggacggtacg gccaagattc agcagcaggg gttggtttgg cgcaagtccc agtgtcttgc
     6301 gggcaaagcg cttggcgatc ggccagaatt ccgcggcatc gaaaccgcac gggatcaccg
     6361 aaatcctggc cggatcggcc ttgtacagcg acagcagatc cgcctcatct tgtggacact
     6421 ccgcaatgat gccgtccgcg ttttcgacga tgcgatcttc tatgtcgatg cgctcggccg
     6481 ggaactggtc agcttcgcgc tggtgcaggc gccgtacgcg acccaatgca tgaaacgtta
     6541 tgacgaacgg aatgcccagg ctctctttca ggatttgtgc caccagcccc gacatgaaga
     6601 aattggcatg aaccagatcg taatggccct cgcgcgtgca aaacgtcgcc acataggccg
     6661 caaactgttg catggtcggc agcaggtctt ccttgcgtac gaattgcggt ggacccgcag
     6721 gaacgtgaac gacgcgcaag ccgtccccac atggcacgac ttcggcgagc aatggattat
     6781 cgcgacgcgt aaatacgtcg acgcgatagc cgagcgaaac gaggtgccgc gcgacctgag
     6841 ccacatatat gttctggccg ccgctgtcca ctccgcctgc cgcagccaaa ggcgacgcgt
     6901 gatcactgat aagggcaatt cttcgaagca tgaggcccat cctgtcgagg gaacaaccga
     6961 tgcgttggta ggggcgcaag caagcaaacg cgttccgtcc ccgtacacgc aacaaaataa
     7021 attcagcgca gcacgcagca acaccacgct gcgttctgcg cctgtaaaag caaaagctgt
     7081 tcctcggttc tgcagcagga cgcaaccgtg cagttgcagc gttgcgcgac gttaagccgc
     7141 ttctatttcg ctggtgccgt ggccgctcga gatagtggcg gaaggtcgtc cgaaattctt
     7201 accggactgt tgtaccgatt acccggtcgc aggcagcgtt tgaaaaatga tttttgcggt
     7261 tcgatgaagt cttgccggtg tatcgaaaac gttacatcac tcgcaatgca ccggcttcac
     7321 aaccgaacgt taattccctt gcacgactac ttgcatatac ttgcctaggc cgcgcatcga
     7381 tcgatggagc agtcattagc agcgcgcgtt tgtgcgccaa tgggagcacc atgcaaggta
     7441 aaagaattgc agtgttggaa agccgctttg gcgagggtct cgtgacgctg ctggaaaaga
     7501 ggggtgccat tgcgtttcat gcacccgctc tcgccgagga accggacatt gattcgaaga
     7561 tgatcgggcg attgcttgag gaatgggcgg caagcccatt tgtactggca atttttcaaa
     7621 ccggtgtcgg cacccgcgcc ttgtttgagg cggccgacag gctgggcatg gcagagtcat
     7681 tgctggctct gttggcgact tgcaaagttg cagtacgggg accgaagccc acggcagcac
     7741 tgcgtgctcg ccatgtgcgc attgatctta tggcgctcga gccttacacc accgcgcaag
     7801 tgctggagtc ggttcgtggt ttcgatttga ccggagcgcg cgtgctggta cagcgatacg
     7861 gtgaaaccaa tcttggactc caggcggcgc tcgaggcgcg gcgagcgcag gttgttgagg
     7921 tgccgaccta ccgctgggca ttgccagccg atactcgccc attggagcgt ctgatcgatg
     7981 gcctcgcgca gggacaactg gatgcggcta tatttaccag tgcatcgcag gtgcgcaatc
     8041 tgtttgcaat cgccgcacag cgcgacattt ctgcactcct gcgccagagc ctgaacacgg
     8101 tgttggttgc atccatcggc ccggtttgca gtcacgcgct tcgggcattc ggcgtccgcg
     8161 tcagcctgga agccactccg cccaaaatgg gaccgctggt ggaaatgttg actacggcat
     8221 ggccgatata aaataactgg tcaattgaac gatatggtgg cggtaagtcg atcaatcgat
     8281 tgctttgtac gccacgccta caatctccag cctttcgtcc ccggcagggg tct
//
LOCUS       DATMZQ010000069         3061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1779495, whole genome shotgun sequence.
ACCESSION   DATMZQ010000069 DATMZQ010000000
VERSION     DATMZQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779495"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VN496_02285"
     CDS             complement(<1..599)
                     /locus_tag="VN496_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF15799.1"
                     /translation="MKLDVLVVGAGFSGCVVAERLASQGFDVLIIDKRSHVGGNAYDT
                     VDRHGVRIHPYGPHIFHTNGVRIAEYLSNFTEWRAYKHRVLAKVGEQFVPIPINIDTV
                     NRLYGLHLDETTIQSFFDSVREPRHPIKTSEDVVLNAVGRDLYEKFFRGYTRKQWGLD
                     PSELAASVAARIPTRTNRDDRYFTDEFQNMPLDGYTAMF"
     gene            complement(747..1970)
                     /locus_tag="VN496_02290"
     CDS             complement(747..1970)
                     /locus_tag="VN496_02290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF15800.1"
                     /translation="MLKRITVDPVSDGPGASAGASLAKLTRAPALRSPVCTDRSPIIV
                     HCHLRWDFVWQRPQQIFSRLAAHHPVLFLEESVREGSKVQLRITEPQTNIVRVVPVLP
                     SDFAASTEAQYRAVLPLLRHALREHPLLAGRFNSPIEWFYSPMTAPAMLGQFDSVRTI
                     YDCMDELANFRFAPPDIARREQFLMSHADVIFTGGYQLFEAKARYHNNVHFYGCGVDV
                     EHYGLARRSETPLPPEVAALPHPVLGYFGVIDERLDYDLLSQIAQAFPQGSLVMVGPH
                     AKVDPGALPKRSNIHWLGQRTYAQLPSLVKSFDVCLMPFALNEATRYINPTKTLEYMA
                     AGKPVVSTAVPDVVRNFTPVVQIARDADDFIAGAMLAYSSPDHALIERGIELAEASSW
                     DAIVAQMRARMIGMI"
     gene            2147..>3061
                     /locus_tag="VN496_02295"
     CDS             2147..>3061
                     /locus_tag="VN496_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HXF15801.1"
                     /translation="MYPRPLMEREDWTSLDGTWKFFYDDERKYVHPTDITEWPMQIVV
                     PFPPESKASGIGDRGLHQGCWYERRFQMRPGPGRVLLHFGAVDYIARVWINGHLVAFH
                     EGGHTPFSAEITPVLNASGEQTITVMVEDDPAELTKPRGKQDWQLEPHSIWYPRTTGI
                     WQTVWLERVNETYISKIRWTPRFDGFAISFEARIKGRQTEDLQVDVTVRHGNRILARD
                     SYQVIDQEVNRRVFLADPGIDDFRNELLWSPERPTLLDAEIRLKSAGVVIDTLRSYTA
                     LRDVSISRDRFLLNGRPYLLRMVLDQGYW"
BASE COUNT          686 a          844 c          897 g          634 t
ORIGIN      
        1 tcaaacatgg cggtatagcc atcgagcggc atgttctgga attcgtccgt gaaataacga
       61 tcatcgcgat tggtgcgggt cggaatgcgc gctgcaaccg atgcggcgag ctctgaagga
      121 tccagtcccc attgcttgcg ggtatagccg cggaaaaatt tctcgtaaag atcgcgtccc
      181 actgcgttca ataccacgtc ctcactggtc ttgatcggat gtcgcggctc gcgtacggaa
      241 tcgaagaagg actggatcgt ggtctcatcg agatgcagtc cgtacaaccg gttcacggta
      301 tcgatgttga ttggaatggg cacgaattgt tcgcccactt tggcaagcac gcgatgcttg
      361 tatgcgcgcc actcggtaaa attggataga tattcggcaa tccttacacc gttggtgtga
      421 aatatatggg ggccgtaagg gtggattctt acaccatgcc ggtcgaccgt atcgtatgca
      481 ttcccgccca catgggagcg cttgtcaata atgaggacat cgaaaccctg gctggcgagc
      541 cgttcggcga cgacacaccc cgaaaaacct gctcctacca ctagcacatc taatttcatc
      601 atcttgttca ctttgcagag acgcggcgag accagctcgt atgcaacgcc gctcgttgga
      661 agggttcagc tgttgctgat gggtatagca ggttccatgc cgatggatcg ggagcacggt
      721 attgcgcttt ttgatcgacg atttgatcaa atcatgccga tcatgcgtgc gcgcatctga
      781 gcaacgattg catcccagga cgaagcttca gcgagctcga ttccacgctc gatgagtgcg
      841 tgatcgggag agctgtaagc cagcatcgca ccggctatga aatcatcggc gtcgcgcgcg
      901 atttgcacga caggcgtgaa attgcgaacg acgtccggca cggcagtcga tacaaccggc
      961 ttgcctgcgg ccatgtactc cagcgtcttt gttggattga tgtacctggt tgcctcgttc
     1021 agtgcaaacg gcatgagaca gacatcgaaa gactttacca atgacggaag ctgtgcatag
     1081 gtgcgctgac ccagccaatg aatattcgac cgcttgggca acgcacccgg gtccactttg
     1141 gcgtgaggcc ctaccatcac caatgatcct tgtggaaacg cctgtgcaat ctgcgacaac
     1201 aagtcgtagt cgaggcgctc gtcgatcaca ccgaaatagc ccagcacggg atgcgggaga
     1261 gcggcgactt ccggaggcag cggcgtttcg cttcgccgcg cgaggccata gtgctctacg
     1321 tccacgccgc aaccatagaa atgcacgttg ttgtgatagc gcgccttggc ctcgaacagt
     1381 tgatagcccc cggtgaaaat tacgtccgca tgcgacatga ggaattgttc gcgcctggca
     1441 atgtcgggcg gcgcaaagcg gaaattggcc aactcgtcca tgcagtcata gatcgtgcga
     1501 acggaatcga attggcccag catcgcggga gccgtcatcg gagaataaaa ccattcgatc
     1561 ggcgaattga accgccccgc cagcaaagga tgctctcgca gggcatgcct gagcagcggc
     1621 aacactgccc ggtactgtgc ctctgtcgaa gcggcgaaat cggacggcag cacgggcacg
     1681 acccgaacaa tgttggtttg cggttcggta atgcgcagtt gcaccttgct gccctcgcga
     1741 accgactctt ccaggaacag aacgggatgg tgcgcagcga gacgggaaaa aatttgctgc
     1801 ggacgctgcc atacaaaatc ccagcgcaga tggcagtgaa cgatgattgg tgaacgatcc
     1861 gtgcaaacag gcgaacgcaa tgccggagca cgcgttagct tagccaggct cgcaccggca
     1921 ctcgcaccgg gcccatcgga caccgggtca acagtaattc gtttcaacat tttcctcttc
     1981 ggggttcgat aaaggccgtg caaacgagca atgtttctga acgcatgcgg ccttaggtgg
     2041 tcttcgtaag acagaccacg taggatggtc cagagttcgc aggccggcaa taggcctgtc
     2101 ggactcactg gttttcgatt acaagaggag gaaaaacttg acaccagtgt atccacgccc
     2161 gctgatggaa cgcgaggatt ggacttcgct tgacggcacg tggaagttct tttacgacga
     2221 cgagcggaag tacgtccatc ccacggatat caccgagtgg cccatgcaaa tcgtggtgcc
     2281 gtttccgccg gaatcgaagg cgagcggcat cggcgatcga gggctgcatc aaggttgctg
     2341 gtatgagcgc cgcttccaaa tgcgtccggg accgggacgc gtgctgctgc attttggcgc
     2401 agtcgactat atcgcgcgcg tctggatcaa cggccacctg gtggcctttc acgagggcgg
     2461 acacacgcca ttcagtgcgg aaattacgcc ggtgctcaat gcctccggtg agcagaccat
     2521 aaccgtgatg gtggaagatg atcccgcgga gttgaccaaa ccgcgcggca agcaggactg
     2581 gcagctcgag ccgcattcca tctggtatcc gcgcaccacc gggatctggc agacggtctg
     2641 gctggaacgc gtaaacgaga cctacatttc caaaatacgc tggacgccgc gcttcgacgg
     2701 ctttgccatc agcttcgagg ctcgcatcaa aggaaggcaa accgaggatt tgcaagtgga
     2761 cgtgacggtg cgccacggta atcgcatcct ggcgcgcgat tcctaccagg tcatcgatca
     2821 ggaagtgaac cgcagagttt ttcttgccga tcccggcatc gatgacttcc gtaacgaact
     2881 gttatggagc ccggagcggc cgaccttgct ggatgcagaa atccgtttga agagcgctgg
     2941 cgtggtgatc gacacgctgc gttcctacac cgccttgcgc gatgtcagca tttcgcgcga
     3001 ccgttttctg ctgaacgggc ggccgtatct gctccgcatg gtactcgacc agggttattg
     3061 g
//
LOCUS       DATMZQ010000070         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3852970, whole genome shotgun sequence.
ACCESSION   DATMZQ010000070 DATMZQ010000000
VERSION     DATMZQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3852970"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1304)
                     /locus_tag="VN496_02300"
     CDS             complement(306..1304)
                     /locus_tag="VN496_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXF15802.1"
                     /translation="MKKVVWGVLSTARIGLQRVVPGMQKSAWCDIRAIASRSEVTARK
                     AADQLGIAKAFGSYDELLADPDIEAIYNPLPNHLHVPLTLRAAAAGKHVLCEKPIALT
                     AAEAEQLKSAAAKVLIMEAFMVRFHPQWLRALDLVRSGSIGTLRAVQMLFCYSNTDAA
                     NIRNMADIGGGALYDIGCYAITAGRFFFGAEPLRGIALIDRDEKFRTDRLTSALVDFG
                     NGRQLTFTVSTQCTPYQRVQLCGTRGRVEIQIPVNAPQGAQTKLFIDDGSSLAGDGIR
                     TEVVPESDQYMLQGEMFSRAVRGEIKLPYGIEDAVRSMRVIDALFRSEQSQRWESV"
     gene            1416..>2009
                     /locus_tag="VN496_02305"
     CDS             1416..>2009
                     /locus_tag="VN496_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXF15803.1"
                     /translation="MEQLSNPAKGRLENGEVAIGIGVRGVRGVEIARLMKSAGYDWLF
                     IDLEHGSTSVETAGQISIAALDAGIAPIVRVPAGELAMGARCLDGGALGVVIPHVDTP
                     EQARAIADAFRFPPLGHRSIGGAYPHFAFTSRPVNEVVTGLDGATLVIAMIETPRAVE
                     NADQIAAVPGLDVLLIGTNDLCLEMGIPGQLDHARVRA"
BASE COUNT          382 a          588 c          593 g          446 t
ORIGIN      
        1 aggaccttca ccccctgcaa tggacctggc atcggcgtga tttcgttatt ctggtttgat
       61 gttcgctgcg cggatgagtt taccccaggc atcgagctat tcgcagaagt tcattgcgaa
      121 aaatacttga gagccgtgaa tatcgtgaat gtcgtgaatg tcgtgacgcc ttaaaccggg
      181 ctgccatttg gctgcaagtg actcagaagt tcattgcgaa aatcttgaca ggtgaaggac
      241 actcatccct ttgacgatat ttacggcatt cacggcattc acggcattca cgatattttc
      301 ggccttcaaa cgctttccca gcgctggctt tgctcggagc ggaacagcgc gtcgatcact
      361 cgcatgctgc gcaccgcgtc ctcgatgccg taaggcaatt tgatttcgcc gcgtacggca
      421 cgcgaaaaca tttccccttg cagcatgtat tggtcgcttt ccggtacaac ttcggtgcgg
      481 atgccgtcac ccgccaggga agagccgtcg tcgatgaaga gcttggtctg tgcgccttgc
      541 ggtgcgttga caggaatctg gatctcaacg cggcctcgcg tgccacacag ttgcactcgt
      601 tgatacggcg tgcactgggt cgataccgtg aaggtcagct ggcgcccgtt gccaaagtcg
      661 accagcgcgc tggtcaagcg atcggtgcgg aatttctcat cgcggtcgat gagcgcgatg
      721 ccgcgcagcg gctccgcgcc gaagaagaag cgcccggccg taatcgcata gcacccgatg
      781 tcatagagcg ctccaccgcc gatgtccgcc atgttgcgga tatttgcggc gtcggtgttg
      841 gaatagcaga acagcatctg gaccgcgcgc aacgtgccga tgctgccgct gcgcaccagg
      901 tccagggcgc gtagccactg cggatggaac cgcaccatga acgcttccat gatgagaacc
      961 ttggcggcag cggatttcaa ctgttcggct tctgctgctg tcagtgcaat cggtttttcg
     1021 cacaatacgt gcttgccggc tgccgcggct cgcaacgtca gcggtacatg caaatgattg
     1081 ggcagcggat tgtagattgc ctcgatatcc ggatctgcca gcaattcatc ataggatccg
     1141 aacgcttttg caatgcccag ctggtcagcc gccttgcgcg cagttacttc cgaacgcgag
     1201 gcgatggccc gaatgtcaca ccaggcgctt ttttgcatac ccggcacaac ccgctgcagg
     1261 ccaatccttg cggtactcag aactccccag acgacttttt tcatgccgtt ccccgttttt
     1321 gcgaaagtgt aactcacccg acgtgcgcgg ccggcacctt ccatcgatca ctctctgtta
     1381 tctttggcga aattctgaac aacgggagaa gccgcgtgga gcaattgagc aatccggcca
     1441 agggccggtt ggaaaacggc gaagtcgcca tcggcatcgg cgtgcgtggg gtgcggggtg
     1501 tcgagatcgc ccgcctgatg aaatcggccg gttacgattg gctgttcatt gacctggagc
     1561 atgggtccac ctcggtggag acggccggcc agatttcaat cgcggcgctg gacgcgggca
     1621 tcgcaccgat cgtgcgcgtt ccggccggcg aacttgccat gggtgcgcgc tgcctcgatg
     1681 gcggcgcact gggcgtggtc attccgcatg tcgatacgcc ggaacaagcg cgcgccatcg
     1741 cggatgcatt tcgctttccg ccactgggtc atcgctccat cggcggtgct tatcctcatt
     1801 tcgctttcac gtcgcgtccg gtgaacgaag tcgtcactgg cctggacggc gctaccctcg
     1861 tgatcgcgat gatcgagaca ccgcgtgcag tggaaaacgc cgatcagatc gcggcagtcc
     1921 ctggtttgga tgtcctgttg attggcacca atgacttatg cctggaaatg gggattccgg
     1981 gccagctcga ccatgcccgg gtgcgagcc
//
LOCUS       DATMZQ010000071         5192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_718984, whole genome shotgun sequence.
ACCESSION   DATMZQ010000071 DATMZQ010000000
VERSION     DATMZQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718984"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VN496_02310"
     CDS             <1..351
                     /locus_tag="VN496_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXF15804.1"
                     /translation="NRTTSWRSIMQKVNRFFRVMFSAVVLFTAMGCANHAHNTQTTSQ
                     RADDSWITSKVKTAFVKDKTLSASDINVETNNGAVALSGFVNDPGDVSHAAEVAGSID
                     GVTSVRNDLHVKQQ"
     gene            413..715
                     /locus_tag="VN496_02315"
     CDS             413..715
                     /locus_tag="VN496_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15805.1"
                     /translation="MKRSMFANIIFFYSALLLTMFVMPMIVTAHSDVRAESQTWDRLL
                     DFPGRGMSEPARGQKQVRPVPESGDTARIVPYDKSFPDAAMITRPTSKTRMKPVKA"
     gene            719..1303
                     /locus_tag="VN496_02320"
     CDS             719..1303
                     /locus_tag="VN496_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15806.1"
                     /translation="MTTIIAGMFDTVDKAAGAVDDLLGGEFNDKDVYSFANNPPGQHA
                     VFPIGGDENKDPGASHAEADAGKGAAVGMGTGAAIGAVAAGPPGAAVGAGLGAYVGSL
                     VGALHGLEGRGSSEQPVRRPAGTLVAVRIESGNAEQSAISTLQTHGAIHIEKAEGVWR
                     DGQWIDFDPLAPPRILDGPNTESSSQRSTEAKEK"
     gene            1306..1515
                     /locus_tag="VN496_02325"
     CDS             1306..1515
                     /locus_tag="VN496_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15807.1"
                     /translation="MRNQEQGRQWNAGLDSGDDDEDFLSDPEVEITEDPADDYADDGD
                     DFADETEATFDELTLMGFRSPFEFA"
     gene            1724..2779
                     /locus_tag="VN496_02330"
     CDS             1724..2779
                     /locus_tag="VN496_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXF15808.1"
                     /translation="MRLNRRVESRRGLFLALESLSGTAALLLDDTCAVEYANAAACTL
                     LKCEDEASLQLKWQQVALLFRLPSQPPLVPKPALHQANIPLADGMRSLSVELQPLHQD
                     AGSGYFAIIRDPAHLDSLERELLQASECRGWAYLRESLLHELKGILNSMQISLELMSD
                     AEAESTAIHPQYERHRRRVASLKEGLTRVDRALRLLPGAEGNGEPSIADFDASELIKE
                     IVATLRQLARRNNVELKVDLPDGPVPARGRRRWIRQALFNIAVHRLNAMRAGGLLIIG
                     ARATDTGLVVHVHDDVAELRGPLVEESTRYFHAGRLNGGVSELQVARAIIEAHHGTLD
                     VDTGSTGGTVVLVCLPP"
     gene            3035..3196
                     /locus_tag="VN496_02335"
     CDS             3035..3196
                     /locus_tag="VN496_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HXF15809.1"
                     /translation="MLQAAAVFFIIAIIAAVFGFGGIAAGATEIAKILFFIFLIVFVI
                     SLVMGLMRR"
     gene            3246..3602
                     /locus_tag="VN496_02340"
     CDS             3246..3602
                     /locus_tag="VN496_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HXF15810.1"
                     /translation="MSVETDAGQINETGGQINTMGQKDKLVEDIKSVMADAQDLLKKA
                     KTSGSESYSVIRAELEERLSESIARLQDVQEELKVRARSAAKATDTYVRDNPWKSVSY
                     VAVAGLIVGLLISRGR"
     gene            3650..5029
                     /locus_tag="VN496_02345"
     CDS             3650..5029
                     /locus_tag="VN496_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HXF15811.1"
                     /translation="MRSYDAVVIGAGQVGESLAIEFARAGLQTVLVDDAEHGGTCTCC
                     GSSKAMLASANVARLARRAAHFGIETGSVEVDFSKVQLRRLSVMDGLRTQAVQALEDT
                     AGLIVLKGEAQFQKAESLVVRETGDDPVELQAARIFINTGARPALPDLPGLSDVPYLT
                     SSSAMDLEQLPDDLLVLGGSYLGVELGQMFRRFGSRVSIIERQSQLLPREDRDIAAAV
                     AENLREDGVGVFLETEAIGAATDSGGGVKVKARTPFGERALTGSHLLIVAGHEPNTRG
                     LNLGAAGIDVDDRGYIKVNERLETSARGVYALGAVNGGPAFAHVAYDDFRVVRANVLD
                     NGHATITRLTCPYTAFLDPQLGRIGLTETQAREQGRQVKVAKLPMNRVARAVELNESG
                     GLMKVIVDSGTQRILGAAVLGVDSREIIAIFQIAMMADMPYTALRDSMFAHPTLVGSL
                     DNLFAALHH"
     gene            5055..>5192
                     /locus_tag="VN496_02350"
     CDS             5055..>5192
                     /locus_tag="VN496_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXF15812.1"
                     /translation="MSQHPFVTDIKALRERARRHIEQGAVTEGYQADRENVIKICNDA
                     LA"
BASE COUNT         1104 a         1447 c         1632 g         1009 t
ORIGIN      
        1 aatcgcacaa caagttggag atcgatcatg cagaaggtca atcgtttctt cagggtgatg
       61 ttttccgctg ttgtgctgtt tacggcgatg ggctgcgcga atcatgccca taacacacag
      121 accacgagcc aacgcgcgga tgattcgtgg atcacttcca aagtaaagac cgcttttgtc
      181 aaagataaaa cgctgagcgc ttcggacatc aatgtggaga ccaacaatgg ggcagtggca
      241 ttgagtggtt tcgtcaacga cccgggcgat gtgagtcatg ccgccgaggt ggcaggcagt
      301 atcgacgggg tgacttcagt ccgaaacgat ttgcatgtga agcagcaata acacggttgt
      361 ttgcaagcag gcgccatttg ggcggcgact gaaaacaggg aaggggataa acgtgaaacg
      421 cagcatgttt gccaacatca tatttttcta ctctgcacta ctgctaacga tgttcgtgat
      481 gccaatgatc gtcaccgcac acagcgatgt cagggccgag tcccagacgt gggaccgctt
      541 gcttgatttt ccgggccgcg gaatgagcga acccgcacgc gggcaaaaac aagtgcggcc
      601 ggtgcccgag tccggcgaca ccgccagaat cgtgccttac gacaagtctt ttccggacgc
      661 cgcgatgatc acgcggccga ctagcaagac aagaatgaaa ccagtcaagg cctgatccat
      721 gacgaccatc attgccggaa tgttcgatac ggtagacaag gcggcgggcg cagtggatga
      781 cctgctgggc ggagaattca acgacaagga cgtttattct ttcgccaata atccaccggg
      841 gcaacatgcc gtctttccga tcggcggcga tgagaacaaa gatcccggtg catcgcatgc
      901 cgaggccgat gccggcaagg gcgctgcggt cggcatgggt accggcgctg ccatcggcgc
      961 tgttgcggcg gggccgcctg gtgcggcagt cggcgctgga ctcggcgctt acgtgggatc
     1021 cctggtcggc gccttgcatg ggctcgaggg gcgtggcagc tcagagcagc cagtgcgccg
     1081 gccagccggg acactggtcg cagtcagaat tgaaagcggc aacgcagagc agtcggccat
     1141 ctcaacgttg cagacacacg gcgccattca tattgaaaag gcggagggtg tctggaggga
     1201 tggtcagtgg attgatttcg accctttagc gccgccacgc atcctcgacg gcccgaatac
     1261 cgaatcgtct tcccaacggt ctaccgaagc caaggagaaa taatcatgcg caatcaggaa
     1321 cagggcaggc aatggaacgc aggtctggac agcggcgatg atgatgagga cttcttgtcc
     1381 gaccccgagg ttgaaatcac cgaggatcca gcagacgact atgccgacga tggcgacgac
     1441 ttcgctgacg aaaccgaggc gacattcgac gaactgacac tgatgggatt tcgctcgcct
     1501 tttgagtttg cctgacctgc acctgctttg tgtgcgggtg gttggctacg tcgagcgcgc
     1561 atccgccgat ggcgcaggct tttgtatccg cagagtcccg ggtaataata gcgcaccatc
     1621 attggtgcta gtttgtcacg ggccggttga agtgtccggc cctgctgaca gccccgtgcg
     1681 aggggcaacg tgcgaggcgc gggtaacgac attgacgatc ggcatgcgat tgaacaggcg
     1741 tgttgaatcc cggcggggtt tgttcctcgc gctcgagtca ttgtcgggaa cggccgcact
     1801 gctgctggat gacacttgcg cggtggagta tgccaatgcc gcggcatgca cgttgctgaa
     1861 atgcgaggac gaagcttcgc tccagctcaa gtggcaacaa gtggcgctgc tgtttcggct
     1921 gccctcccaa ccgccgctcg tgccgaaacc cgcgttacat caagccaaca ttccgctcgc
     1981 ggatggcatg cgctcgttga gcgttgagtt gcaaccactg caccaagatg ccggctcggg
     2041 ttatttcgcg attatcaggg atcctgccca tctggacagc ctggagcgcg agctattgca
     2101 ggcaagtgaa tgccggggat gggcttacct gcgcgagtcg ctgctgcacg agctgaaggg
     2161 aatcctgaat tccatgcaga tctcgctgga gctgatgtcc gatgcagagg cggaatcgac
     2221 ggccattcac ccccagtacg agcgtcatcg gcgccgcgtc gcctcgttga aggaaggcct
     2281 cacgcgcgtc gatcgtgctt tacggctgct gccgggtgcc gaagggaatg gcgagccgtc
     2341 cattgccgat tttgacgcaa gcgagttgat caaggaaatt gtggcgactc ttcggcaact
     2401 cgcgcgccgg aacaacgtgg agctgaaggt cgatttgcca gacgggccgg ttccagcgcg
     2461 cgggcggcgc cgctggatcc ggcaagcgtt gttcaacatc gcagttcatc gtttgaatgc
     2521 aatgcgtgca ggcggcctgc tgatcatcgg agctcgcgca acggacaccg gcctggtcgt
     2581 gcacgtgcac gatgatgttg cagaattgcg cgggccgctg gtagaggaaa gcactcgcta
     2641 ttttcatgcc ggccgcctga atggcggcgt aagcgaattg caggtcgccc gtgccatcat
     2701 cgaagcccat cacgggacgc tggacgtgga caccggaagt acgggcggca ccgtagtact
     2761 ggtctgcctc ccgccctgat ggcgcaatgg tcccccaaca gtcagccgcc gggtaatttt
     2821 tgcaaggacg cgtttccgcg cgtgaggacc gttcggtaag cgttacagca atcttgcaaa
     2881 tcattccggg ccgcgcgcag ccggaaattt aattctgttc gaaatgagcg gtttgagccc
     2941 actctcgttg ccatatcgca atggcatgga cgttgctcga tgacagcagg caatttctgc
     3001 cggatggcag cgatgcgtac ccatggaggc aaacatgctt caagctgctg ctgtgttttt
     3061 catcatcgct attatcgctg cggttttcgg attcggcggc attgccgcgg gcgccacgga
     3121 aatcgcgaaa atcctgttct tcatcttcct tatcgtgttc gtgatcagcc tggtcatggg
     3181 cctgatgaga cgttgaggac ggcaccgaac aaacaaggca agtcaactta tacacaggag
     3241 tcgatatgag cgtcgaaacc gatgccggac aaatcaacga gacgggtgga cagatcaata
     3301 caatgggtca gaaggacaaa ctggtcgaag atatcaagtc cgtcatggcc gatgcgcagg
     3361 acctgctgaa gaaagcaaag acttcaggtt cggaaagcta ttccgtgata cgcgcggaac
     3421 tcgaggagcg cctgtccgag tccattgccc gcctgcaaga cgtgcaggaa gaactgaagg
     3481 tgcgggcgcg cagcgctgcc aaggcgactg acacctacgt gcgcgacaat ccatggaagt
     3541 ccgtgagcta tgtagccgtc gccggtctga tcgtcggatt gctcattagc cgcggtcgtt
     3601 aaccccacgc gctgctgtcc ggcgcgcatt cctggcaggc atccacgcga tgaggagcta
     3661 tgacgcggtg gtgatcggag cgggccaggt aggggagtcg ctggccattg agttcgcgcg
     3721 cgcgggactg caaacagtgc tggtggatga tgccgagcat ggcgggacgt gtacctgttg
     3781 cgggtcctcc aaggcgatgc tcgccagcgc caatgtcgcc cgcctcgcgc gcagggcggc
     3841 gcactttggc atcgaaaccg gcagcgtgga agtcgatttc agcaaggtac agctccgcag
     3901 gctctcggtg atggacgggt tgcgtaccca ggcggtgcaa gcattggagg acacggccgg
     3961 attgatcgtg ctcaaagggg aagcacaatt ccagaaggca gaatccctgg tggtgcggga
     4021 gaccggtgat gatccggtcg aactgcaagc tgcgcgcatc tttatcaata ccggcgcacg
     4081 gccggcctta ccggatcttc cgggattgag cgatgtgccc tatctgacgt cgagcagtgc
     4141 aatggatctg gaacaactgc ccgacgattt gctggtgttg ggaggcagtt atctcggcgt
     4201 ggaactgggg caaatgtttc gccgcttcgg ctcgcgcgtc tcaataattg aacggcagag
     4261 ccagctgctg ccgcgcgagg accgggatat cgccgccgca gtggccgaaa atctgcgcga
     4321 agacggtgtc ggcgttttcc tggaaaccga agccatcggc gcggcgaccg actctggcgg
     4381 cggcgtgaag gtcaaggcgc gcaccccttt cggtgagcgc gctctaacag gatcacattt
     4441 gctcattgtg gcaggacatg agcccaatac ccggggattg aaccttggcg ccgccggcat
     4501 cgacgtcgac gaccgcggct acatcaaggt aaacgagcga ctggaaacca gcgcgcgcgg
     4561 cgtctatgcg cttggtgcgg tgaacggcgg gccggcgttt gcgcatgtcg cctacgacga
     4621 ttttcgcgtc gttcgagcga acgtgctgga caacgggcat gccacgatca ccaggttgac
     4681 ctgcccgtac acggcctttc tcgatccgca gctcggccgc atcggcctga cggagacaca
     4741 ggcccgcgag caagggcgcc aagtaaaggt cgccaagctg cccatgaaca gggttgcgcg
     4801 cgccgtggaa ctaaatgagt ccggcggact catgaaggtc atcgtcgatt ctggcacgca
     4861 acgcatcctc ggcgccgccg tgctcggggt cgacagccgc gaaatcattg ccatatttca
     4921 aatcgcaatg atggctgaca tgccgtacac cgcactgcgc gacagcatgt tcgcgcatcc
     4981 cacgctggtc gggtcgctgg acaacctatt cgcagcgctc catcactgaa catgtcttta
     5041 aacaggagat gaccatgagc caacatccgt ttgtcaccga tatcaaggcg ctgcgtgagc
     5101 gggcgcggcg ccacatcgag caaggcgcgg ttacagaagg ctatcaggca gaccgcgaaa
     5161 acgtaatcaa gatctgcaat gatgccctgg ct
//
LOCUS       DATMZQ010000072         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3853316, whole genome shotgun sequence.
ACCESSION   DATMZQ010000072 DATMZQ010000000
VERSION     DATMZQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853316"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VN496_02355"
     CDS             <1..1977
                     /locus_tag="VN496_02355"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXF15813.1"
                     /translation="LVVRGYGSASILATGANTQMGRIGKALEALEIQDTPLQVQTARF
                     VRIFAAIGLLLCLAVVVLYGTLRADWLAGLLAGITLAMAVLPEEFPVVLTVFLALGAW
                     RISRRKVLTRRMPAIETLGSATVLCVDKTGTLTENRMVVAELAAQGHTHDAARGALPA
                     SVRPVAHIAALACELNTFDPMERAIVAYALAHDAETVRMRERWHLVKEYDLTRDLLAV
                     THCWQKPDGGEFVVAAKGAPEAIANLCNADRPMQQSILEQTAHIAARGRRVLGVAQAR
                     FQGGDFPARPNEFAFTFVGLIAMADPVRPTVPQAVRECHDAGMRVIMITGDYPGTAQS
                     IADEIGLQSPGGVRTGAQLERLTPAQLRACVADVSVFARVVPEQKLALVNALKANGEI
                     VAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREAASLVLLEDDFKSIVEAVRLGRRIY
                     DNIRHAMSYLIAVHVPTAGMALFPLVFGWPLVFAPVHIVFLEFVIDPACSIAFEAEPA
                     HPGIMKRPPRDPKEPLLGGSLLTGAFLQGLTLLVAISLLYWLVLAYGYGEQRARAVAF
                     TALVLGNVGLILTNRSRSRSMFATLRMSNRALGWVVGGALAGLAAALYIPALQIIFRF
                     DSLSWSDLLLGFAAASAGISWTEIVKLMPGRKRR"
     gene            complement(1996..>2507)
                     /locus_tag="VN496_02360"
     CDS             complement(1996..>2507)
                     /locus_tag="VN496_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXF15814.1"
                     /translation="DLLAVQSATQDAARRRLSHFAAALVALIFLMALSGGFVAGIRAG
                     FAYNTFPLMNGHLVPPEIFTLDPWWSNLFSNIATVQFNHRMIAWLLMLFVPAFWIAAQ
                     RVPMAPRTRLATHVLLGMLVVQVALGISTLLLGVPIPLAAAHQAGAVLLLCAALWSAH
                     ELRGRPRAS"
BASE COUNT          449 a          729 c          824 g          505 t
ORIGIN      
        1 ctcgtggtcc gcggctatgg cagcgcctcg atcctggcca cgggtgccaa cacccagatg
       61 ggtcgcattg gcaaagcact cgaggcgctg gagattcagg acacgccgtt gcaggtgcag
      121 acggcccgct tcgtgcggat tttcgcggcg atcggacttt tgctatgcct tgcggtggtg
      181 gtgctgtacg gcaccttgcg cgctgactgg ctggccgggc tgctggccgg tattacgctg
      241 gcgatggcgg tgttgccgga agaatttccg gtcgtgctca cggtgttcct ggcgttgggc
      301 gcatggcgga tttccaggcg caaggtgctg acgcggcgca tgccggcgat cgaaacgctc
      361 ggctcagcga cggtattgtg cgtggacaag actggcacct tgaccgaaaa ccgcatggtc
      421 gttgcagaac tcgcggcgca gggccataca cacgacgccg ctcgcggtgc cttgcctgca
      481 agcgtgcggc cggttgccca cattgccgcg ctcgcgtgcg agctgaacac gttcgatccg
      541 atggagcgtg caattgtggc ctatgccctt gctcacgacg ccgaaaccgt gcgcatgcga
      601 gagcgctggc acctggtgaa ggaatacgat ctcacgcgtg atttgctggc ggtaacgcac
      661 tgctggcaaa aacccgatgg aggcgagttc gttgttgcag ctaagggcgc gccggaagcg
      721 attgcgaatc tgtgcaatgc cgacaggccg atgcagcagt ccatacttga acaaacggcc
      781 catatcgccg cgcgcggccg gcgggtgctc ggtgtggcgc aggcgcgttt tcagggtggc
      841 gatttcccag ctcggccaaa cgaatttgcg ttcacttttg tcggactaat cgcaatggcg
      901 gacccggtaa gacccaccgt gccgcaagcg gtcagggaat gccacgacgc cggcatgcgc
      961 gtgatcatga tcacaggaga ttaccccggg acggcgcagt cgattgccga tgagattgga
     1021 ttgcaatccc ctggcggagt gcgtaccggc gcgcagctcg agcgcctgac gcccgcgcaa
     1081 ttgcgtgcat gcgtggcgga cgtcagcgtc tttgcgcgcg tggtgccgga gcagaagctt
     1141 gcgctggtga atgcgctgaa ggctaacggc gaaatagttg ccatgaccgg cgacggcgta
     1201 aacgatgcac cggcgctcaa agcggcgcac atcggtattg ccatgggcgg gcgcggcacc
     1261 gacgtcgcgc gcgaggcggc atcgttggtg ctgctggaag acgacttcaa gtctatcgtt
     1321 gaagccgtgc ggctgggccg tcgaatctac gacaatatcc gccacgcaat gagttatctg
     1381 attgcggtgc acgtccctac cgccggtatg gccctttttc cgctggtgtt cggctggccg
     1441 ctggtgtttg cgccggtgca tatcgtattt ctggagttcg tgatcgatcc ggcatgctcg
     1501 atcgccttcg aggcagaacc cgctcatccg ggcatcatga aacgtccacc gcgtgacccg
     1561 aaggagcctt tgcttggtgg ctctttgctg acgggtgctt ttctgcaagg cctcacgctg
     1621 ctggtcgcaa tctcgctgtt gtactggctc gtgctcgcat atggttatgg ggaacaacga
     1681 gctcgcgcgg tggcttttac cgcgctggtg ctcggcaatg taggtttgat tcttaccaat
     1741 cgctcccgca gccgcagcat gttcgctacg ctgaggatgt cgaaccgcgc gctgggttgg
     1801 gtggtgggcg gtgccttggc ggggctggcg gcggcgcttt acataccagc gctgcagatt
     1861 atatttcgct tcgatagctt gtcctggtcg gacctgctcc ttggtttcgc ggccgcttca
     1921 gccggcattt cctggaccga aatcgtgaag ctgatgccgg ggcgcaagcg gcgctgaaga
     1981 cattcgttta gtggattaac ttgctctcgg gcgcccgcgc aactcgtgag cgctccagag
     2041 cgccgcgcag agcaacagca cggctccggc ctgatgcgca gcagccagtg gaatcggcac
     2101 gcccagcagt agcgtcgaga tgccgagcgc cacttgtacg accagcatgc ccagcagcac
     2161 atgcgtggcg agcctcgtgc gcggcgccat tggtacgcgc tgggccgcaa tccagaacgc
     2221 ggggacaaat agcatcagca gccacgcgat catgcggtga ttgaactgca cggtcgctat
     2281 gttgctgaac agattgctcc accacgggtc cagtgtgaag atttccggcg gaacgagatg
     2341 tccgttcatc agcggaaacg tgttgtaagc gaaaccggcg cgaatgccag caacgaagcc
     2401 accgcttaat gccatcagaa aaatgagcgc aaccagtgcg gccgcgaaat ggctcaatct
     2461 gcggcgtgcc gcatcctggg tagcgctttg gacggccaac aggtcca
//
LOCUS       DATMZQ010000073         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1409421, whole genome shotgun sequence.
ACCESSION   DATMZQ010000073 DATMZQ010000000
VERSION     DATMZQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409421"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VN496_02365"
     CDS             <1..299
                     /locus_tag="VN496_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15815.1"
                     /translation="GSFTIKRFDRQAFSEPAAVLTVKQRQIFMTGRSVFNRQWAAVTS
                     LNGDWGPGAYFHCRPLQRLPHRVRSRRATARIERTIAFHARAPEHPWYGCARRP"
     gene            196..1314
                     /locus_tag="VN496_02370"
     CDS             196..1314
                     /locus_tag="VN496_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXF15816.1"
                     /translation="MGSGRGAPPHASNEQLLSMLVRLSIPGTDAHGGPNPHPNYGDQI
                     QNRSLDGRSVDFAYSGTAVPNEADLFLDWEDLIVAFADGETVSLRKPKLRIENLKFGE
                     LGSQTMLSLRIAQPLVGAGFLEAVPEDTLLALAEKQRAYNINGRPNRVWDEVHKRMSF
                     GRFGWKANTPNLKQQIAAAAVGDMGVNSNLFPEQNCPPVQVICAKHLPGNMPEIIDHE
                     INALELWLQGLAVPARRGVDDAAVKRGGRFFSQAQCDVCHVPTLKTGKFENLPQLSNQ
                     VFHAYTDLLLHDMGEELADGRPDFAAGPRDWRTPALWGLGLSQTVGGSISLLHDGRAR
                     NVSEAILWHGGEAQAARDAFRHMSREEREALIRFVEAI"
     gene            complement(1441..1767)
                     /locus_tag="VN496_02375"
     CDS             complement(1441..1767)
                     /locus_tag="VN496_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15817.1"
                     /translation="MLTRLIVNLYAWIMEIYLWLVLLISGVAGYHYTIPLLKDVGAIP
                     ESEAVWKIYGALAFAVAALLLSAVATGPFLVLVEIRKSIRALETKKSSDGSSELLPAE
                     RREPLF"
     gene            complement(1781..2047)
                     /locus_tag="VN496_02380"
     CDS             complement(1781..2047)
                     /locus_tag="VN496_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15818.1"
                     /translation="MAQAVKFLIVSRSIVASCLFWRGAMIQVAAGHLTRMQALESELL
                     AKGYKEVGHQSELGPMEYRKTNDFASGKWSFSLTWNDSTPSVSR"
BASE COUNT          481 a          636 c          646 g          471 t
ORIGIN      
        1 ccggctcatt caccatcaaa cgtttcgacc gccaggcttt ttccgaacca gcggccgtgc
       61 ttactgtcaa gcaacgccag attttcatga ccgggcgcag cgtgttcaat cggcagtggg
      121 ccgcggtaac ttcgcttaac ggcgactggg gccctggggc ctactttcat tgccgaccgt
      181 tgcagcgctt gccacatcgg gtccggtcgc ggcgcgccac cgcacgcatc gaacgaacaa
      241 ttgctttcca tgctcgtgcg cctgagcatc cctggtacgg atgcgcacgg cggccctaat
      301 ccgcacccga attatggcga tcagatacag aaccgttcac tggatgggcg cagcgtggat
      361 ttcgcctatt ccggcacagc ggtgccgaat gaagccgacc tgttcctcga ttgggaggac
      421 ttgatagttg catttgcgga tggtgaaacg gtttcactgc gcaagccgaa actgcgcatc
      481 gagaacctga agttcgggga gcttggcagt caaaccatgc tctcgcttcg cattgcgcag
      541 ccgctggttg gcgcaggctt cctggaagcc gtacccgaag acacattgct ggcgctggcc
      601 gaaaaacagc gggcctacaa tattaacggc aggccaaacc gcgtctggga tgaggtgcat
      661 aagcgcatgt cgttcgggcg cttcggctgg aaagccaata ccccgaatct gaagcaacag
      721 atcgctgctg ccgccgtggg cgatatgggc gttaattcaa atttgtttcc cgagcagaac
      781 tgtccgccag tgcaggtcat ctgcgccaaa catctgccgg gcaacatgcc tgaaatcatc
      841 gaccacgaaa taaatgcgct cgagctgtgg ctgcaaggcc ttgccgttcc cgcgcggcgc
      901 ggtgtcgatg acgcggccgt gaaacgcggc ggcagatttt tcagccaggc acagtgcgat
      961 gtgtgccacg tgccgacgct caagacgggc aaattcgaaa acctgccgca attatcgaat
     1021 caggtgttcc acgcgtacac ggacctgctg ttgcatgaca tgggcgagga attggcggat
     1081 ggccggcctg actttgcggc gggaccgcgt gactggcgca caccggcgtt gtgggggttg
     1141 ggattgtcac agaccgtggg cggcagcata tcgctgttgc acgacggccg tgcgcgcaat
     1201 gtgagcgaag caatcctttg gcatggtggc gaagcgcaag ccgcacgcga cgcttttcgc
     1261 cacatgagcc gggaagagcg cgaagcgctg atccgattcg ttgaagctat ctagaatggc
     1321 agtctgccgt atcgaggcct gcttcgcggg atctgtgttg tgtaatggcg gccctccagc
     1381 gggcgatctg catagcgccg ctcgtctcta actctgccag caccggggct gggcctcttt
     1441 tcaaaaaagt ggttccctgc gttcggccgg tagaagttcg ctgctgccgt cgctgctctt
     1501 cttcgtctcg agggctctta tcgactttcg gatctcgaca agcacgagaa atggtcctgt
     1561 agccacggcc gacagtagca atgcagctac agcaaaggcg agagcgccat agattttcca
     1621 tactgcttca ctctcaggaa tcgctccgac atccttgagc aaaggaatgg tgtagtggta
     1681 cccagcgacg ccagatatca ggagcactaa ccacagataa atctccatga tccaggcgta
     1741 gaggttgact attaatctag taagcattcg ggcccctttc tcaccgtgaa acgctcggag
     1801 tcgaatcgtt ccatgtcagc gaaaaggacc atttgccgct ggcgaaatcg tttgttttgc
     1861 ggtactccat cggccccagt tcagattggt gccccacttc cttatatcct tttgctaaaa
     1921 gttcggattc gagcgcctgc attcttgtaa ggtggccagc agcgacttgg atcatggctc
     1981 ctcgccaaaa aagacaagaa gccactatgg aacggctgac gataaggaac ttgaccgcct
     2041 gcgccaagcg ctgatccgga cgcgccatcc gaggggagcc agctaccgca acaatttttt
     2101 tggcgcgcca agacaagtga ctggcgtcag cgggcaagcg aagtttctgc cgatccggga
     2161 tgcttcatgt cgctaaactt tcccaatagg cgagaatgca acagatcacc ggcacttatc
     2221 gcagagaaaa aggc
//
LOCUS       DATMZQ010000074         2774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4171748, whole genome shotgun sequence.
ACCESSION   DATMZQ010000074 DATMZQ010000000
VERSION     DATMZQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4171748"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VN496_02385"
     CDS             complement(<1..217)
                     /locus_tag="VN496_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HXF15819.1"
                     /translation="MSYALLKLTHIAAVIVSFSLFVMRGLWMMYAPQKLQGRAIKIVP
                     HVIDTILLASAIALAAMSGQYPFAQNWL"
     gene            complement(214..693)
                     /locus_tag="VN496_02390"
     CDS             complement(214..693)
                     /locus_tag="VN496_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15820.1"
                     /translation="MADYFLVRALHIVGVVLWIGGVAFVTLVLLPAVKRLRMPVERVE
                     FFEQVEAGFSRQAKFTTLLTGLSGFYLVHRLNAWQRFGDAQFWWMHAMVLVWVAFTLV
                     LFVLEPWWLHRWFKARAQRDADGTFSIIARAHWALLAVSLITIAAAAAGSHGWSFAR"
     gene            891..1160
                     /locus_tag="VN496_02395"
     CDS             891..1160
                     /locus_tag="VN496_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15821.1"
                     /translation="MRADLEVLGSNNGQYAFQFPLATNHLFQGWADVFLTTPRQGIRD
                     SYLSGGMKIEKMQFLAESLRRGALPAMMVVGRTLIQVKDRRAPRA"
     gene            1209..1754
                     /locus_tag="VN496_02400"
     CDS             1209..1754
                     /locus_tag="VN496_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15822.1"
                     /translation="MERRSVTVALSLVLIVVSTTRADDSDLVQGEKLYSSECKICHGA
                     ISQKAGLDAPGRSQPSLRLALHHVQESTMFDIPVGLIADPAGAAGTALSGAAGDPIAF
                     TVPYGPNLRGVIGRPAGTVEGWRYSPTFLKTLKGMEWTEAALDVWMTGTQAWVPGVYM
                     FYKQPNAEIRRKIILYLKANP"
     gene            2037..>2774
                     /locus_tag="VN496_02405"
     CDS             2037..>2774
                     /locus_tag="VN496_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXF15823.1"
                     /translation="MILNDGSRKPVGVWLLLCCVLVFGMVVVGGITRLTHSGLSMVEW
                     QPLVGTIPPLNEQQWQNVFGQYQATPEYQKINHGMSLDEFKGIFWWEYFHRLLGRAIG
                     VAFFVPLAWFLVRGKISGLLALKLGGIFLLGALQGAIGWYMVKSGLVDNPRVSQFRLT
                     AHLGMALLILAAMLWTALDLLAVQSATQDAARRRLSHFAAALVALIFLMALSGGFVAG
                     IRAGFAYNTFPLMNGHLVPPEIFTLDPW"
BASE COUNT          572 a          815 c          826 g          561 t
ORIGIN      
        1 tcagccagtt ctgtgcgaag ggatattgcc cggacatcgc agcgagcgcg atcgcgctcg
       61 ccagcagaat ggtgtcgatg acatgtggaa cgattttgat ggcgcgaccc tgcaattttt
      121 gcggcgcata catcatccac aggccgcgca taacgaacag cgagaaactg acgatcacag
      181 cagcgatatg cgtgagcttc agcagcgcgt agctcaacgg gcgaagctcc agccatggct
      241 gcctgcggca gcagccgcga tcgtaatcag gctcacggcg agcaacgccc aatgcgccct
      301 ggcgatgatg gaaaacgtcc cgtccgcatc gcgctgcgca cgcgccttga accagcggtg
      361 cagccaccag ggctccagta cgaatagcac gagcgtgaac gcgacccata ccagcaccat
      421 cgcgtgcatc caccagaact gcgcatcccc aaaccgctgc catgcgttga gtctgtgcac
      481 gagatagaaa ccgctcaacc cggtcaacag cgtcgtgaat tttgcctggc gcgaaaatcc
      541 cgcttccacc tgttcgaaga attccacgcg ttcaaccggc atgcgcaagc gctttacagc
      601 gggcagcagc accagcgtca cgaaagccac tccgccgatc cacagcacga cccccacaat
      661 gtgaagcgca cggacgagaa aataatctgc cattgatgtt gcctgagtgc ggtgacttcg
      721 agggggaaaa ctggtggagc gctttacgcg actaaaccag ctaccggcat gctaatcgaa
      781 aataattgat tcaggtcaag gtgcccagcg ttaccgctgg aacagaatcc atcaagggcc
      841 ctggctcgtc gcgcgcatcc tgcgccggcg ccaaatgaca tgacacttat ttgcgcgccg
      901 acctggaagt actgggcagc aacaatggcc agtatgcctt ccagtttccg ttggcgacca
      961 atcacctgtt ccagggctgg gcagacgtgt ttctgaccac gccgcgccaa ggaatccgcg
     1021 acagttattt gagcggtggc atgaagatcg aaaagatgca gttcctggcc gagtcgctgc
     1081 ggcgaggtgc cctgccggca atgatggttg ttggacggac attgatccag gtcaaggacc
     1141 gccgtgcgcc ccgggcataa gttggacaag caatattccg gtcccacgaa ctcgatgagg
     1201 gaatcgccat ggaacgccga tccgtaaccg ttgcactgtc gctggtcttg attgtcgtgt
     1261 cgactacccg agccgatgat tccgacctgg tgcaaggtga gaaactctat tcgtcggaat
     1321 gcaagatctg ccacggcgcc atttcgcaga aggcgggcct tgacgcgccc gggcgttcgc
     1381 aaccgtcctt gcgcctggcg ctacatcacg tgcaggagtc gacgatgttc gatatcccgg
     1441 tgggattgat cgcggatccc gcaggggcag cgggaaccgc actttccgga gcagcaggcg
     1501 atccgatcgc tttcactgtg ccctatgggc cgaatctgcg cggcgtcatc gggcgtccgg
     1561 cgggaaccgt cgaagggtgg cgctattcgc ctacatttct caagacgctc aaaggaatgg
     1621 agtggaccga agccgcgctc gacgtctgga tgaccggtac gcaggcctgg gtacccggcg
     1681 tttacatgtt ctacaagcag ccgaatgcgg agatccgccg caagattatc ctgtacctga
     1741 aagccaatcc gtaatccggc acggcgccaa acgaaatgaa accggcacaa cggtcggctg
     1801 ctgacgagcc gtcgtgacac cgggttcaat agcccgccgg gctgccttcc cggtgaaccc
     1861 atgcgagcat cggccagtac acaatcagat aatctggcgc attgccggac acggcatgac
     1921 gccttcaatt tccctacgac ttgcgcgtcg cctggtggca atgagtgagc gtgttgccgg
     1981 aaatggttag aattctgctt gcgcatgtcc tgcgcttttt ttattttcga tgacgaatga
     2041 tcctgaatga cggttcccgc aaaccggtgg gagtgtggct gctgctgtgt tgcgtcctgg
     2101 tgtttggcat ggtggtcgtt ggcggcatca cgcggctgac gcactccggc ctgtcgatgg
     2161 tggagtggca accgctggtc ggcacgatac cgccgttgaa cgagcaacag tggcagaatg
     2221 ttttcggcca ataccaggcc acgcccgagt atcagaaaat caaccatggc atgtcgctcg
     2281 acgagttcaa gggcatcttc tggtgggagt attttcaccg cctgctggga cgtgcgatcg
     2341 gcgtagcgtt ctttgtgcct ctagcctggt tccttgtgcg cggcaaaatc agcggcttac
     2401 tggcgctgaa gctgggcggc attttcctgc tcggcgcact gcagggcgcg atcgggtggt
     2461 acatggtcaa gagcggcctg gtggataatc ctcgcgtcag ccagttccgg ttgaccgcgc
     2521 atctcggcat ggcgctcctg attctggcgg cgatgctgtg gaccgcgctg gacctgttgg
     2581 ctgtccaaag cgctacccag gatgcggcac ggcggagatt gagccatttc gcggccgcac
     2641 tggttgcgct catttttctg atggcactaa gcggtggctt cgttgctggc attcgcgccg
     2701 gcttcgctta caacacgttt ccgctcatga acggacatct cgttccaccg gaaatcttca
     2761 cgctggaccc gtgg
//
LOCUS       DATMZQ010000075         3018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3163016, whole genome shotgun sequence.
ACCESSION   DATMZQ010000075 DATMZQ010000000
VERSION     DATMZQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163016"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VN496_02410"
     CDS             <1..652
                     /locus_tag="VN496_02410"
                     /inference="COORDINATES: protein motif:HMM:NF026070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15824.1"
                     /translation="LAWAVIAAGTCATYGGIHAMEGNPTGCMGLPDYLGWQWKSTASI
                     PIVCVPGCPVQPDNFMETLLYLLHMAAGRAPTISLDDALRPTWLFGASVHEGCDRGGY
                     YEQGEFAEEYGSPLCIVKLGCWGPVVQCNVGKRGWMAGIGGCPNVGGICIGCTMPGFP
                     DKFMPFMDQPPGSLLSSSAVMTYGRAIHALRRFTQSSLNKEPSWRRKTTKPDLHNG"
     gene            671..2278
                     /locus_tag="VN496_02415"
     CDS             671..2278
                     /locus_tag="VN496_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXF15825.1"
                     /translation="MVPAWSVESVAQSEAKNAELTPGEAETLLVDLAAVFLGNSAPEL
                     GSTDLSRDQEEQLPNVSAIYRALVEQIPAVVFMAHLDRGIGEAYVSPQIQSTLGFSQA
                     EWLEDPIRWYRQIHPDDKQRWSVEAAHMFLSGTPLRSAYRVMARDGRVVWFHCEAKMI
                     RRADGRPWFIHGVGFDITELKRTEEALQEERNVVSAILHTVGALVVVLDPEGRIVRFN
                     RACEQTAGYSFAEIKGKYFWDLCVIPEEAERFKIIVEQLRMDHNAKDYETSWVTRDGA
                     RRLIAWSSTVLPGGDGAPAYIIATGIDITERKRLEKAILEISADEQRRIGQDLHDGLG
                     QHLTGIAFMSKVQEQKLAEKGLKEAAEAARIVKLVNEAINKTRELAHGLLPVVAESQG
                     LMSALRRWAVEVEDVFRISCQFHCDSPVLIHDAIMSTHLYRIAQEAVNNAMRHGHATE
                     IVITLSAGNGQGTLVIEDNGPGIAERVDEPSANHSGMGLQIMNYRARMICGSLKLQNG
                     KTRGTVVTCAFPLNRNNEALKQARYDH"
     gene            2268..2960
                     /locus_tag="VN496_02420"
     CDS             2268..2960
                     /locus_tag="VN496_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF15826.1"
                     /translation="MITKQTNTNPATKCSKVFLVDDHPIVRQGLTLLINQEADLAVCG
                     QAEDMHSALQAIHDSRPDIMIVDISLNGPDGLELLKSIRVKYPDMPVLILSMHHESFY
                     AERALRAGANGYIMKQEATEKVLVALRRILGGEIYVSDRVGNHMLQHYIRGASPFPQS
                     SVAELTDRELEVFRLIGEGHGTRQIAEELHLSVKTVESYQAHIKEKLSLRSARELVQH
                     AIQWTVSEKKSA"
BASE COUNT          671 a          782 c          919 g          646 t
ORIGIN      
        1 gctggcctgg gccgttatcg ccgcgggaac gtgcgcaacc tatggtggca tccatgcgat
       61 ggagggaaat cccaccgggt gtatgggtct tccggattat ctcggatggc agtggaaatc
      121 cacagcgagc atcccgattg tttgtgtccc cgggtgtccc gtgcagccgg acaacttcat
      181 ggagacgctg ctctaccttc ttcatatggc tgccgggcgt gcaccgacca tctcgctcga
      241 cgatgcgctg cgcccgacct ggctgtttgg ggcgtccgtg cacgagggtt gcgatcgcgg
      301 aggctactac gagcagggag agtttgcgga ggaatacgga tcgccgctgt gcatcgtaaa
      361 gttggggtgc tggggcccgg ttgtacagtg caacgtgggc aagcgcggat ggatggctgg
      421 gatcggcgga tgtcccaatg ttgggggcat atgcatcgga tgtaccatgc cggggtttcc
      481 tgacaagttc atgccgttca tggaccagcc gccgggttcg ctattgtcat cgagcgctgt
      541 gatgacctat ggccgcgcca ttcatgccct gcgcagattt acccagtcgt cgctaaacaa
      601 agagccaagt tggcggcgca aaacaaccaa gcctgatctc cataatggct gaccttgttg
      661 gggttgaaat atggttcccg cgtggagtgt cgaatcggtc gcgcaatccg aagccaagaa
      721 cgcggaattg accccaggag aagctgagac tcttctggtt gatcttgctg cggttttttt
      781 gggaaactcg gcgccggaat tgggatcgac cgatctcagc cgagaccagg aagaacaact
      841 tcccaacgtc tctgccatct accgggcact ggtcgaacag atccccgccg ttgttttcat
      901 ggcccacctt gatcgtggca tcggcgaagc ctacgtcagc ccgcaaattc aatcgacgct
      961 cggtttttcc caggccgagt ggctggaaga tcccatccgc tggtaccggc agattcatcc
     1021 cgacgacaaa caaaggtgga gtgtcgaagc cgcccatatg tttctgtcag gcacgccgct
     1081 gcgctccgcc tatcgtgtca tggcgcgaga cggccgggtg gtgtggtttc actgcgaagc
     1141 caaaatgatc cggcgggcgg acgggcggcc ctggtttatc catggcgtcg gtttcgatat
     1201 cacggaactc aagcggacag aggaagcatt gcaggaagaa cggaacgtag tttccgccat
     1261 tcttcacacc gtaggagctc tcgtcgtggt gctcgatcca gagggacgga ttgtccggtt
     1321 caaccgcgcc tgcgagcaga cggctggtta ttcatttgcc gaaataaagg gaaaatattt
     1381 ctgggactta tgcgtgattc cggaggaggc cgagcgtttc aagattattg tagagcagct
     1441 gcgcatggat cataatgcca aagactatga gacatcctgg gtcacgcgcg atggcgcacg
     1501 gcgattgatt gcgtggtcca gcactgtttt gccgggcggc gatggtgcgc ccgcctatat
     1561 catcgcgacg ggaatcgaca ttacagaacg caagcgactg gagaaagcga ttctggaaat
     1621 tagcgccgac gagcaacggc ggattggaca ggacctgcat gatgggttgg ggcagcattt
     1681 gaccgggatt gctttcatga gcaaagtgca ggaacagaag ttggcagaga agggattgaa
     1741 ggaggccgcg gaagcggccc ggatcgtcaa gctggtaaat gaggccatca acaaaacgcg
     1801 agagctggcg catggtcttc ttcccgtggt ggcggaatcg caaggcttga tgtccgcgct
     1861 caggcggtgg gcggttgaag tggaagatgt ctttcgcatc tcgtgccagt tccattgcga
     1921 tagtccagtc ctcatccacg acgccatcat gtccacccat ctttatcgga ttgcccagga
     1981 ggccgtgaac aacgccatgc ggcacggaca cgccactgag atcgtaatta ctctatcggc
     2041 tggcaacgga cagggaacgc tggtcatcga agacaatggt cctggtatag ccgagcgagt
     2101 tgacgaacca tctgccaacc attccggaat ggggctacag atcatgaatt atcgtgctcg
     2161 catgatctgt ggttcgctta aactccagaa tggcaagact cgcgggaccg tggttacttg
     2221 cgcatttcca ctcaatagga acaacgaagc gttgaagcag gctagatatg atcactaaac
     2281 agaccaacac taatccggcc acaaaatgca gcaaagtatt tttggttgat gatcatccca
     2341 ttgtgcgcca gggcctgaca ctgctgatca atcaggaggc agatctggcg gtctgtggtc
     2401 aggccgaaga catgcattcg gcattgcagg cgatccatga ttctcgcccg gacatcatga
     2461 tagttgacat ctcgctcaat ggacctgatg gattggagct gttgaagagc atcagggtga
     2521 agtatccgga catgccggtg ctgattctgt ccatgcatca cgagtccttc tatgcggaaa
     2581 gagcgctgcg ggcgggcgcg aacggctaca tcatgaagca ggaggcgacg gaaaaggtcc
     2641 tggttgcttt gcgccgcatt ctggggggag agatttacgt cagcgatcga gtgggcaacc
     2701 acatgctgca gcattacatt cgcggcgcca gcccttttcc gcaatcttcc gtggcggaac
     2761 ttacggaccg cgagctcgaa gttttccgct tgatcggcga aggtcatgga acgcggcaga
     2821 ttgccgaaga acttcatttg agcgttaaga cggtcgagtc gtaccaggcg cacatcaaag
     2881 agaaactatc tttgcgaagc gctcgcgaac tggtgcagca cgcaattcaa tggacggtga
     2941 gcgagaagaa gagcgcgtga gctccgccag aaacacgcca tagtttttcc ctccttatcg
     3001 agcggttcgg cgctgaag
//
LOCUS       DATMZQ010000076         7691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_720890, whole genome shotgun sequence.
ACCESSION   DATMZQ010000076 DATMZQ010000000
VERSION     DATMZQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720890"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /gene="lptC"
                     /locus_tag="VN496_02425"
     CDS             <1..442
                     /gene="lptC"
                     /locus_tag="VN496_02425"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXF15827.1"
                     /translation="DGTRRYALAAKRMVHYPDDNSTELELPKLVYFDYQRAPVTIRSE
                     TAEVVQGGDDVFFRGDVQIIRSAYERNAELGVFTSFLHVIPDQDMAKTDKPVRMVEGI
                     STASSVGLEFNSVTREIKLLSQVKASYETPPKRSMRRRKLPDAR"
     gene            432..1031
                     /gene="lptA"
                     /locus_tag="VN496_02430"
     CDS             432..1031
                     /gene="lptA"
                     /locus_tag="VN496_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HXF15828.1"
                     /translation="MPGRGLVLALTAALAVGMNFPLLSLAEKADSDKPVNLEADRVDL
                     DDAKKEAVFVGNVTLTQGTLSIKADKIIVKQDAAGFQYGIAYGKPAHFRQKREGYDEY
                     IDGFSERLEYDGKADKVQMFTNARIQRGGDEVRGDYISYNAVTEFFQVIGGAKSASPT
                     GNSEGRVHAVIQPKPKGGNPAPSGGVELKPSGTVQTPKQ"
     gene            1063..1785
                     /gene="lptB"
                     /locus_tag="VN496_02435"
     CDS             1063..1785
                     /gene="lptB"
                     /locus_tag="VN496_02435"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXF15829.1"
                     /translation="MSQLRAEKLKKRYKARTVVKDVSLDVLSGEVVGLLGPNGAGKTT
                     CFYMMVGLVSLDGGDLFMDDHLLTHTPIHRRARMGLSYLPQEASIFRKLTVAENILAV
                     LELRHLDESSIQAELDVLLHDLHIAHLRNSPAISLSGGERRRVEIARALATQPRFILL
                     DEPFAGVDPIAVLDIQKIIRFLRERDIGVLITDHNVRETLGICDRAYIINDGSVLAAG
                     KPEEIVYNESVRKVYLGEHFRL"
     gene            1847..3295
                     /locus_tag="VN496_02440"
     CDS             1847..3295
                     /locus_tag="VN496_02440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXF15830.1"
                     /translation="MKHSLQLKLSQHLTLTPQLQQSIRLLQLSTLELNQEIETFLQEN
                     PLLERDDEEEAQTPPSPLNSTTTTTTSTESEAPPSSSDNGPPDEPAAAESDWSVDDGY
                     GSFSGQDGDEDGEAPQASAEPAKLRDHLLDQINLMQMPDRDKRIVAFLIDNLDDDGYM
                     TQDLAELSALLPVELEIDAEELTIGLKYLQSLDPPGIGARNLSECLALQLETLPEDTQ
                     YRSEALLVVRQHLDVFAARDYFRLKKLLRCDDDVLRAVQAMIIGLNPRPAGNFSGGET
                     RYIIPDVVVRKIKGLWVTALNPEAMPKLRINRMYADILHRNRDASFQQLAGQLQEAKW
                     LIKNVQQRFDTILRVSQAIVDRQRHFFEHGEVAMRPLVLREIAETLGLHESTVSRVTT
                     QKFMLTPRGIFELKYFFGSHVSTETGGACSATAIRALIKQMVGEEDTRKPLSDSQISE
                     VLGKQGIMVARRTIAKYRESLQILPVNLRKSI"
     gene            3321..3644
                     /gene="raiA"
                     /locus_tag="VN496_02445"
     CDS             3321..3644
                     /gene="raiA"
                     /locus_tag="VN496_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXF15831.1"
                     /translation="MNLQLSGHHLELTPAIRSYVIDKLTRISRHFDHVIDVGVILSVN
                     KLDHKAEATVHVRSKDIHVECIDANMYAAIDGLADKLDRQILKHKEKIVDHHEEESIR
                     NLPLP"
     gene            3666..4151
                     /gene="ptsN"
                     /locus_tag="VN496_02450"
     CDS             3666..4151
                     /gene="ptsN"
                     /locus_tag="VN496_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HXF15832.1"
                     /translation="MSLISRLLPSANITLDLDVTSKKRVFEHVGLLFENALGIGRGQV
                     FDALFARERLGSTGLGHGIAIPHGRIKGLREAAGAFVRTRGPIPFEAPDGNPVNLIFV
                     LLVPEKANDLHLQILSELAQMFSDKEMRTQLAALPDAAAVQQVISKWEPHAPGQRRAA
                     V"
     gene            4120..5061
                     /gene="hprK"
                     /locus_tag="VN496_02455"
     CDS             4120..5061
                     /gene="hprK"
                     /locus_tag="VN496_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HXF15833.1"
                     /translation="MPQVSVAQLFDDNADALKLAWISGPREPSPYLDSSEINQASQGL
                     IGHLNLVHPNWIQIFSRTEAAYFASLGENDQREALGRLAGSGLVCIILSEQETWPATL
                     AQFAATRNIPLLSSPLHSLQIIWVVRTYLGRALAEFVTRHGVFLDVLGMGVLITGQSG
                     VGKSELALELISRGSGLVADDVVELYHIAPETLEGRSPELLKDFLEVRGLGMLNIRTI
                     FGETAVRTKKNLKLIVQMEKPVGGVIPGLDRLPLNASAEDIMGISIRKVVLPVAAGRN
                     LAVLAEAAVRNYVLQLRGIDSTQEFIQRQEKQLSRRS"
     gene            5082..5918
                     /gene="rapZ"
                     /locus_tag="VN496_02460"
     CDS             5082..5918
                     /gene="rapZ"
                     /locus_tag="VN496_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXF15834.1"
                     /translation="MQLVVITGLSGSGKSIALNVLEDSAYYCVDNLPAKLLNGLLQFL
                     EHEGYARVAVSIDVRSGATLSELPDYLSQLKKRGIQVHLIFLDSKTETLVKRFSETRR
                     RHPLADRDQTLPECIEQERELLANLSGLGQRIDTSDLHPSVLRSWIKDLLLGGESGLH
                     LLFQSFGFKHGIPLDADMVFDVRCLPNPHYDPELRPLTGKDAPVIAYLEANANAREML
                     ADIRDYVEKWLPCFVRDNRAYLTIAIGCTGGRHRSVYFAESLATYFRGTYSVLVRHRE
                     TV"
     gene            5950..6582
                     /locus_tag="VN496_02465"
     CDS             5950..6582
                     /locus_tag="VN496_02465"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HXF15835.1"
                     /translation="MKRNKPESITLALTGASGMPYGVRLLECLLGAGIRVNLLFSQAA
                     QIVASQEMGLALPARAKDAEKFFSLRSNVPPGQLVAYGREEWFAPIASGSNPADAMVV
                     CPCSMGTLAAIAAGLSTNLIQRAADVTLKEARKLVLVPRETPLSAIHLENMLKLSRAG
                     AIILPANPGFYHRPHTAQQLVDFVVARILDQIGVEHGLMPRWGEPNTSDG"
     gene            6610..7185
                     /locus_tag="VN496_02470"
     CDS             6610..7185
                     /locus_tag="VN496_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXF15836.1"
                     /translation="MSVLPIFPLNTVLFPGALLPLKIFEQRYVDMTKACVRDHSDFGV
                     CRIREGREVGLPAVPEHIGCSAQIMDWEMPHLGVFHLRTRGQVPFRILQHATQAGGLI
                     QADIEWLPESLGEINPKSLSLCRRVLEQIVQRIGANYFLPPLAYDDPRWISYRLSEIL
                     PLQAEEKQILLAMRDDGERLERLHVHLKRAT"
     gene            complement(7186..>7691)
                     /locus_tag="VN496_02475"
     CDS             complement(7186..>7691)
                     /locus_tag="VN496_02475"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXF15837.1"
                     /translation="GLMDLGAEVCIRRRPRCECCPLSGMCVAKRDDRIANFPAPRPKK
                     TLPEKSTVMLILQRAGEVLLEKRPAPGIWGGLWSFPELANLEDVVGVVHRRFGAQATS
                     EGILAAVRHGFTHYALTITPALLRVTRLELRAQSAGQAWMTPADAINSAVPAPVRSLL
                     RLLESGG"
BASE COUNT         1711 a         2170 c         2158 g         1652 t
ORIGIN      
        1 cgacgggacg cgtcgttatg cactggcagc gaagcggatg gtgcattatc cggatgacaa
       61 tagcactgaa cttgaattac ccaagctcgt ttactttgat taccaacgtg ctccggtaac
      121 cattcgttcc gaaacagcgg aagtcgtaca aggtggtgat gacgtattct tccgcgggga
      181 tgtgcagatt atccgctccg cttacgagcg caatgcggaa ctcggagtgt tcaccagttt
      241 cctgcacgtc atacctgacc aggacatggc aaagacggac aaaccggtgc gtatggtgga
      301 aggaatctcc acggcgagtt cggttggctt agaattcaac agtgtgaccc gcgagataaa
      361 actgctgtcc caggtgaaag ccagctatga aacgccgccc aagcgatcca tgcgcaggag
      421 aaagcttccg gatgcccggt agagggctgg ttcttgcgct gacggcggca ttggctgtcg
      481 ggatgaactt tcccttactc agtctggccg aaaaggcgga tagcgacaaa cccgtcaacc
      541 ttgaggcaga ccgcgtcgat ctcgacgatg caaagaaaga agccgtattc gtcggaaacg
      601 tgacgctgac ccaaggaaca ctgagcatca aggcagacaa gattatcgtc aaacaggatg
      661 ctgcgggttt ccagtacggg attgcttacg ggaagccggc tcattttcgc caaaagcgtg
      721 aaggctacga tgaatacatc gatggtttct ccgagcgtct cgaatacgat ggcaaggccg
      781 acaaagtgca gatgttcacc aatgcccgca tccagcgcgg cggcgatgaa gtgcgcggag
      841 actatatttc gtacaacgcc gtgaccgaat tctttcaggt tatcggtggt gccaaatcgg
      901 cttcccccac cggcaattca gaagggcggg tgcatgccgt catacagccc aagcccaaag
      961 ggggaaaccc tgccccatcg ggcggtgtgg agctgaaacc ttcaggaacg gttcaaaccc
     1021 ccaaacaatg accggcatgc atgcgtcctc caggacgact agatgagcca actgcgcgcc
     1081 gaaaaactga aaaaacgata taaggcccgg acggtcgtca aggacgtctc gctggacgtg
     1141 ttgagcggcg aggtggttgg tttgcttggg ccgaacggtg ccggcaagac cacctgcttt
     1201 tacatgatgg tgggcctcgt gtcgctggat ggtggcgact tattcatgga cgaccatctg
     1261 cttacccaca cgcccattca tcggcgcgcc cgcatgggct tatcctacct gccacaggag
     1321 gcgtctatct tccgcaaact gaccgtcgcc gaaaatatcc tggccgttct ggaattgcgt
     1381 catttggacg aaagcagcat tcaggcagaa cttgatgtgc tgttgcacga tttgcacatt
     1441 gcgcacttgc gtaacagccc ggccatcagt ttgtccggtg gcgaacgccg ccgcgttgaa
     1501 attgcgcgtg ctcttgccac ccagccgcgc ttcattctgc tggacgaacc atttgctggc
     1561 gtcgatccca tcgccgtgct ggatatccaa aaaatcatcc gttttttgcg cgagcgcgat
     1621 atcggagtgc tgatcacgga tcataacgtc cgggagacac tggggatttg cgatcgtgcc
     1681 tacattatca acgacggctc agtactggcc gcaggcaaac cggaggaaat cgtttataat
     1741 gaaagtgtaa ggaaagtata tctaggcgag catttcaggc tctaagcgct tgccgccctc
     1801 gagttctggg ccgttggtta tccggcctga ttttgatagt tagccgatga agcattccct
     1861 tcagctcaaa ctttcgcagc acctgactct cacgccgcaa ctgcagcagt ccattcggct
     1921 tctacagctg tcgacgctcg aactgaatca ggaaatagag acatttctgc aggaaaatcc
     1981 gttactggag cgagacgatg aggaagaggc gcagacaccg ccgtcgcctt tgaactccac
     2041 aaccacgact accacctcaa ccgagtccga agcgccaccg tcatccagcg acaacgggcc
     2101 gccggacgag ccggccgctg cagaatccga ctggtctgtg gatgatggct acggatcttt
     2161 cagcggacag gatggcgacg aagacggcga ggctccgcaa gcaagcgcgg agccggcgaa
     2221 gctgcgtgat catttgctcg atcagatcaa tttgatgcaa atgccagacc gggacaagcg
     2281 catcgttgct ttcctgattg acaatctgga cgacgatggc tacatgacac aagatctggc
     2341 cgaactgtca gcgctattgc cggttgaact cgagattgat gccgaagaac tgacaatcgg
     2401 gctgaagtac ctccaaagtc ttgatccgcc cggaatcggc gcccgcaacc tgagcgagtg
     2461 cctggcccta caactggaaa cgctgcccga ggacacacag taccggtcag aggcattgct
     2521 ggtcgtgagg cagcatctgg acgttttcgc cgcgcgcgat tacttccgcc taaagaaact
     2581 tctgcgctgc gacgatgacg ttcttcgtgc cgtacaggca atgatcatcg gcctcaaccc
     2641 ccgcccagcc ggcaattttt ctgggggaga aactcgctac atcataccgg acgtagtggt
     2701 gcgcaagatc aagggactgt gggttaccgc attgaaccct gaggcaatgc cgaagctgcg
     2761 cattaaccga atgtacgcgg atattctgca tcgcaatcgc gacgccagtt ttcagcaact
     2821 cgcgggtcag ctgcaggaag caaaatggct gatcaagaat gttcagcagc gcttcgatac
     2881 gatcttgcgg gtctcacagg ccatcgtaga ccggcaacgc cattttttcg aacacggcga
     2941 agtagcgatg cgtccgctcg tgttgcgcga aattgcggag acgttaggcc ttcatgagtc
     3001 gacggtatcg cgcgttacga cgcaaaaatt catgcttacg ccgcgcggga tttttgaact
     3061 gaaatacttt tttggcagcc acgtctccac ggagaccggg ggcgcttgtt cagcgactgc
     3121 cattcgagcg ctgataaaac aaatggtggg cgaagaagac acccgcaagc cgctaagcga
     3181 cagtcaaatc tccgaggtac ttggcaagca aggcatcatg gtggcgcgcc gcactatcgc
     3241 gaaataccgg gaatctctgc agattcttcc ggttaatctt cgtaaatcga tctgatcaca
     3301 catctaacgg aggaaagcct atgaatcttc agctatccgg ccatcatttg gaactgactc
     3361 ccgcgatccg ttcgtacgtc atcgacaagc tcacgcgcat ttcacgtcat tttgatcatg
     3421 tgattgacgt cggtgtcatt ctgtctgtca ataagctgga ccataaggca gaggcgacgg
     3481 tgcatgtgcg cagcaaggat attcatgtcg agtgcataga cgcaaacatg tacgccgcca
     3541 tcgacggcct tgcggacaag cttgaccggc aaattctgaa gcataaggaa aagatcgtcg
     3601 atcaccatga ggaagaatcc attcgcaatc ttcccctgcc ctaggctggt ctcgccggcg
     3661 cttccatgag tctgatctcg agactgttgc cgtccgccaa cattactctg gaccttgacg
     3721 tcacgagcaa aaaacgggtg tttgagcacg tcggcctgct gttcgaaaac gcgctaggga
     3781 tcggtcgcgg ccaggtgttc gatgccttgt tcgcacgaga acgacttgga tcaactggat
     3841 tggggcatgg cattgcgatc ccgcacggac gcatcaaggg attgcgcgaa gccgccggcg
     3901 catttgtacg cacccgcggg ccaatcccgt tcgaagcccc ggatggaaat ccggtgaatc
     3961 tgattttcgt gctgctcgtt ccggagaagg ccaacgactt gcatctgcag atcctttcag
     4021 aacttgcaca aatgttcagt gacaaggaaa tgcgtacgca gttggccgcg ctgccggatg
     4081 cggcggcggt acagcaggtg atcagcaaat gggaacctca tgccccaggt cagcgtcgcg
     4141 cagctgtttg atgacaatgc cgacgcgctg aagctggcct ggatctccgg tccgcgcgag
     4201 ccttcaccgt atctcgacag cagcgagatc aaccaggctt cacaaggact catcggccac
     4261 ctgaacctgg tccatccaaa ctggatccag attttcagcc gcactgaagc cgcctatttt
     4321 gcttcgctgg gcgagaacga tcagcgcgag gcgcttggcc ggcttgcggg cagcggcctg
     4381 gtgtgcatta tcctcagcga gcaggaaacg tggcccgcca cactggcgca atttgccgct
     4441 acgcgtaata tcccattgct ttcttcccct ttgcacagcc ttcagattat ttgggtcgtg
     4501 cgcacctatc tcggacgcgc cctcgccgaa ttcgtaaccc gtcacggcgt ttttctcgac
     4561 gtattgggga tgggggtact gatcaccggc cagagtggcg taggcaagag cgagctggcg
     4621 ctggaactca tctctcgggg cagcgggtta gtcgctgacg atgtcgtcga gctctatcac
     4681 atcgcgcctg agacactgga aggccgcagt cctgaattgt tgaaggattt tttggaagtg
     4741 cgtggcctgg gaatgttgaa catccgcacg atatttggcg aaactgccgt gcgtacaaag
     4801 aagaatctga agttgattgt gcagatggaa aagccggtcg gtggcgtgat cccgggatta
     4861 gatcgcctgc cgcttaatgc cagcgctgag gacatcatgg gtataagcat tcgcaaggtc
     4921 gtacttccgg ttgcggctgg ccgcaatctg gcggtactgg cggaagccgc cgtgcgcaat
     4981 tacgtgctgc aattgcgcgg catagacagc acccaggaat tcattcaacg ccaggaaaag
     5041 cagttgagcc ggcgcagcta aggaccggcg cgccgagaat catgcaactc gtcgtcatca
     5101 ccggactttc cggttccggc aagagcatcg cgctcaacgt gctggaagac agcgcctact
     5161 attgtgtcga caatctgccg gccaagctgc tcaatggcct gctgcaattc ctggaacacg
     5221 aaggctacgc gcgtgtcgca gtgagcatcg atgtccgcag cggcgcaacg ctgtccgaat
     5281 tacctgacta cctgtcccag ctcaagaaac gcggcattca ggtacatctt atctttctgg
     5341 attccaagac cgaaacactg gtcaagcgct tttccgaaac gcggcgtcgg catccgcttg
     5401 ccgatcggga tcagacgctt ccggaatgca tcgagcaaga gcgcgaactg ctcgcgaacc
     5461 ttagcggact gggccagcgt atcgatacca gcgatttgca tcccagcgtg ctgcgttcct
     5521 ggataaaaga tctactgctc ggcggcgaat ccggcttgca tttgttgttc cagtcttttg
     5581 gattcaaaca tggcatcccg ctggatgccg acatggtgtt tgatgtgcgt tgcctgccca
     5641 atccgcatta cgatccggaa ttgcgtccgc taactggcaa ggatgcgcca gtgattgcct
     5701 atctcgaggc caatgccaac gcacgggaaa tgttggccga tatccgagac tacgtcgaaa
     5761 aatggttgcc ttgtttcgtg cgcgacaatc gcgcgtatct gaccattgcc attggctgca
     5821 ctggcggccg ccatcggtcg gtttacttcg ccgagtcctt ggccacttat tttcgcggaa
     5881 cctatagcgt gctggtccgc catcgcgaga cggtatgact cgcctgtcgc gtacgcgacc
     5941 ttctcaatca tgaagcggaa caaaccggaa tcgatcacgt tagcattgac tggcgcatca
     6001 ggcatgccat atggcgtgcg gctattggag tgcctgctcg gcgccggtat ccgggttaat
     6061 cttttgttct cgcaagcagc tcagatcgtc gcaagtcaag aaatgggctt ggcattgcct
     6121 gcgcgtgcca aggatgccga gaaatttttc agtttgcggt caaacgtgcc acccggacag
     6181 ctggtggcat acggccgtga ggaatggttc gcgccaatcg catccggttc caatccggcg
     6241 gatgccatgg tggtctgtcc gtgttccatg ggcacgcttg cagcaatcgc ggccggcttg
     6301 tccaccaatc tgatccaacg tgctgcagat gtgacattga aggaagcgcg caaactggtt
     6361 cttgtgccgc gcgaaacacc gttgtccgcg attcatttgg aaaacatgct caagctgtcg
     6421 cgtgccgggg cgattattct cccagccaat cccggctttt accatcggcc gcataccgcc
     6481 caacagcttg tcgattttgt cgttgcccga attctcgacc agattggcgt cgagcatggc
     6541 ttgatgccgc gttggggcga acccaatact agcgacggct gatcgaaata ttcacgcctg
     6601 gcaagtgaaa tgagcgtcct gccgattttt ccactgaata ccgtgctttt cccgggcgcc
     6661 ttgctgccgt taaagatttt tgagcaacgc tacgtcgata tgaccaaggc atgcgtccgc
     6721 gatcattcag acttcggcgt ttgtcgcatt cgcgaaggcc gcgaagtggg acttccggct
     6781 gtgccggagc atataggctg cagtgcgcaa atcatggatt gggaaatgcc gcatcttggc
     6841 gtttttcatc tgcgtacacg cggacaggtc ccatttcgta ttctgcaaca tgcgacccag
     6901 gctggcggac tgatccaggc cgacattgaa tggctacccg aaagcttagg ggagatcaat
     6961 ccaaaatcgc tctcgctatg ccgccgcgtt cttgaacaaa tcgtgcaaag gatcggcgcc
     7021 aattactttc taccgccgct tgcgtatgat gatccgcggt ggatcagcta ccgcctatcc
     7081 gaaattcttc ccttgcaggc cgaagaaaag cagatcctgc tggcaatgcg cgacgacggc
     7141 gaacggctgg agcggctgca tgtgcatttg aagcgagcga cctgatcaac cgccgctctc
     7201 caacaggcgc agaaggctgc gcaccggcgc cggcacggcg ctgttgatcg catcggcggg
     7261 cgtcatccag gcctgccccg cagattgcgc gcgcagctcc agtcgcgtta cacgcaggag
     7321 tgccggagta atcgtgagcg cgtaatgggt gaagccgtgg cgcacagcgg ccaagatacc
     7381 ttccgaagtt gcttgcgccc cgaagcgcct gtgcacgacc ccaaccacat cctccaaatt
     7441 cgccagctcc ggaaagctcc acaatccacc ccagatcccg ggcgccggcc gtttttccag
     7501 taatacttcc cctgcgcgct gcaggatcaa catcacggtg gatttttccg gcaatgtttt
     7561 tttcggccgt ggggccggaa agttggcgat acgatcgtct cgcttcgcca cacacatgcc
     7621 gcttagcgga cagcactcac agcgtggccg gcggcggata catacttcag cgccgagatc
     7681 catcaagccc t
//
LOCUS       DATMZQ010000077         9015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3165557, whole genome shotgun sequence.
ACCESSION   DATMZQ010000077 DATMZQ010000000
VERSION     DATMZQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165557"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /gene="mtnA"
                     /locus_tag="VN496_02480"
     CDS             <1..934
                     /gene="mtnA"
                     /locus_tag="VN496_02480"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXF15838.1"
                     /translation="RGAPLIGATAAYGMALAMSANSSDANLAQAADFLGKTRPTAINL
                     RWAIRRMTRLLQDVLPAARVDAAWKEAQAIADEDIALNLAIGQHGLAMLREVAKRKAN
                     INVLTHCNAGWLATVDVGTALAPIYLAHDAGMSVHVWVDETRPRNQGLLTTWELSEHG
                     VPNTLVADNAGGHLMQRGQVDLVMVGADRVTGAGDVCNKIGTYLKALAAKDCGVPFYA
                     AAPSPTIDWEIRNAMTDIPIEERGGDELLVVRGQQAADQGSTQVRIAPPEVRAANPAF
                     DVTPGRLVTGIITDLGIAIPTRRGLDKLYRTRAA"
     gene            937..1578
                     /locus_tag="VN496_02485"
     CDS             937..1578
                     /locus_tag="VN496_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXF15839.1"
                     /translation="MTELQLRKETIATALHMNALGINRGKSGNVSARWKHGFLVTPSA
                     LPYEEMRPSDIVFIDTKGRSKGIRKPSSEWRFHFDIYRHKPTCQSVVHTHSSFATTLA
                     CLGMGVPAFHYMVAVAGGKDIRCAPYATFGTQALSDHAIAALEQRAACLLANHGMIAT
                     GSSLKNALALAVEVEALCEQYWRALQISTPRLLSDQEMALVVEKFVAYNSLSH"
     gene            1620..3521
                     /gene="phaC"
                     /locus_tag="VN496_02490"
     CDS             1620..3521
                     /gene="phaC"
                     /locus_tag="VN496_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HXF15840.1"
                     /translation="MTEKPNSAPTSADPTELARTFAEIALRSKHLVETHLAAHAGQVG
                     EEASDELGVGKAFSELAGRLMSDPFKLAEMSMRMWHDYFNLWQNTLLKASGAEAPSVA
                     QPGKSDTRFRSELWENNLLFDYIKQSYLIAAQNIQKSVAEVDGLDPVTARKVKFFTRQ
                     YIDALAPTNFVLTNPEVLKTTIETGGKNLLDGLNHLLADMDRGDGKLAISMTDPKAFT
                     MGENVASTPGKVIFQNDLMQLIQYQPTTSSVYKTPLLIVPPWINKYYILDLREKNSFI
                     RFALDQGITVFVISWVNPDSRHAGKTFDDYLLEGPRAAMDAIEKATGEHDINMIGYCL
                     GGTLTACLLAWMAAKGETRVKSVTFFTTLIDFSEPGELGVFVDEEVVSKLEKKMAERG
                     YLEGSEMASTFNMLRANDLIWSFVVNNYLLGKEPFPFDLLYWNSDATRMPAKMHTFYL
                     RNMYIKNKLREPGGITLAGESIDVSRIKLPAYFISTLEDHIAPWKSTYLSAQLLTGPV
                     RFTLGGSGHIAGVVNPPSANKYWYWTNDSLLDTAEAWLAAADKRPGSWWTDWASWFAG
                     FGGELIPARQPGQGKLAVIEDAPGSYAKFRLDQAPAEHAGMPDAPARRKTPRNKPAAI
                     RDIRKPFQR"
     gene            3767..5458
                     /locus_tag="VN496_02495"
     CDS             3767..5458
                     /locus_tag="VN496_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HXF15841.1"
                     /translation="MRELLSRFRRLFLFAALFSLVMNLLLLVPALYMLQIFDRVIAGR
                     SNETLVLLTISVGVALIMMMALDVLRARLLAAGGLMLDNWLGPTVVRTLYDRARRPGG
                     MEPVNGLSDVAALRGFLTGPGVLALVDAPWFPIYLIVIFIFHPLLGAVASAGALSLLS
                     LTIINERLTRPPLERLQSQGRRASRLIDAGLRNAEIVSALGIADDLTRRWQMQNDQVL
                     DLQHQASRRATAMSGMTRFLRQLLQVMMLGVGAYLVIDRHVSSGVMIAATILLGRALA
                     PVETLIAGWKNLVDARSALRRLDALLANTAQRESTQLPAPEGRLVFDRVVFGVKGRER
                     PIIAGISLELASGESLGIVGPSASGKSTLARLAVGVWEPTMGTVRIDGSDLANWPRER
                     IGPFVGYLPQDIELFAGTVAENIARMGEVDSDAVVEAAQQARAHELILRLPEGYDTPV
                     GEGGTLLAGGQRQRIGLARALYRRPRLVVLDEPTAHLDAEGEEALVAALRYLKQQHAT
                     IILITHRPSLLQNADIDKVAVLKTGALDLFGTRGEIMGKVTRGALTVASQMGKAS"
     gene            5460..6782
                     /locus_tag="VN496_02500"
     CDS             5460..6782
                     /locus_tag="VN496_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXF15842.1"
                     /translation="MTTTAQRLPKDLYALEARRLIGLGLTVLVVLVISIGLWMVITPL
                     TGAAIAPGAVKVDMNRKTLQHQEGGIIKEILVRDGSHVEAGETLLVINDVRVDANYDL
                     LITQLDSEMAKASRLFAESTLDAGVAYAKELESRSEESRVKELMRRENAVFATRRQML
                     DTQIKLLKEQIQEAKAEAIALGEQVEAEKRAVAWQQEELLANQQLAEKGFMSNTRLLA
                     LQRGVADYEARLGEHRATLAQARQREGDFELRIASLRNNYMKEAADELKEATAKTYDL
                     RERLRPSLDAAQRQRVIAPVSGDVVDLRVTTVGAVVGPRESLLDIVPANPELIVEARV
                     RPEDITYVKIGGDADVRLSAFKQRITPTVTGKVTYVSADSLTDNKTGATYYLTHVKLT
                     PEALREAGDLKLQAGMPAEVFVKTPARTPLQYLLDPLTGFLQHSFREP"
     gene            6930..7769
                     /locus_tag="VN496_02505"
     CDS             6930..7769
                     /locus_tag="VN496_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXF15843.1"
                     /translation="MKLSKEQSEQFDRDGYLFFPSLFTAQEVKVLLDEVPAIYAQRRA
                     ENVREKDSDAVRTNFAAHMYNRRFAKLARHPRMVEPIMQLFGEAVYMHQFKVNGKMAF
                     DGDVWQWHQDYGTWKNDDQMPEPRAMNVAIFLGEVNEFNGPLMFIPGSHKLGVLEAEH
                     DTSTTSYPLWTINHDTIRKLVERGGLVAPKGPAGSMILFHSCLVHASNCNLSPWDRIN
                     VYLSLCAVSNHIRRFKRPEYIAHRDFTPIHCLPDDCLIKDYDVALPWKDGTPAEALKP
                     LAA"
     gene            7778..>9015
                     /locus_tag="VN496_02510"
     CDS             7778..>9015
                     /locus_tag="VN496_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXF15844.1"
                     /translation="MNLFAKLREREAQGKPLRVGLIGAGKFGAMYLAQVPKTPGVHLS
                     GIADLSPANAKASLQRVGWKAERYAAPTLDAAFSSGLTHVSDDWQALVEHPLIDIIVE
                     ATGNPIAAVEHALAAFKHGKHVVMVTVEADAMCGPLLARRARNAGVVYSLAYGDQPAL
                     ICDLVDWARASGFSVVAAGRGHKWLPHFAHSTPETVWGYYGLTPEQAKTGGLNPKMFN
                     SFLDGSKPAIECTAVCNATGLTPAPYGLLFPPASVDDIPNVMRPIAEGGHLHHKGQVE
                     VISSLETDGRVIPYDIRFGVFVVFEGETEYIRNCFKEYMVRTDASGRYACLYKRWHLI
                     GLEVGISVASVGLRGEPTGCPTGFRADVVATAKRNLKAGEILDGEGGYTVVGKLMPAD
                     TSITIGALSLGLAHGVKMLRP"
BASE COUNT         1871 a         2673 c         2756 g         1715 t
ORIGIN      
        1 acgcggcgca ccgcttatcg gcgcgacggc cgcatatggc atggcgctgg cgatgtcagc
       61 caactcgtcc gacgcaaatc tggcgcaggc cgcagatttt ctcggcaaga cgcgcccgac
      121 agcgatcaat ctgcgctggg caatccggcg catgacgcga ctgctgcaag atgtgcttcc
      181 tgctgctcgc gtcgatgccg cctggaagga agcgcaagcg attgcggacg aagacattgc
      241 actgaatctc gccatcggcc agcacggcct ggcaatgctg cgcgaggttg ccaagcgcaa
      301 ggcaaacatc aacgtgctga cccactgcaa cgccggttgg ctggcgactg tcgatgtagg
      361 tactgcattg gcgccaatct atctcgcgca cgacgctggc atgtcggtgc acgtgtgggt
      421 tgacgagact cgaccacgca accagggatt gctgaccacc tgggaacttt ccgagcacgg
      481 cgtgcctaac acgctggtcg ctgacaacgc cggcgggcat ctgatgcagc gcggtcaagt
      541 cgatctggtc atggtgggcg cggatcgcgt tactggggcg ggtgacgttt gcaacaagat
      601 cggcacttac ctgaaagcgc ttgctgcaaa agattgcgga gtgccgttct acgccgcggc
      661 gccttcgcca accattgatt gggaaatccg gaacgcgatg acggatatac cgatcgagga
      721 acgtggcggc gacgagcttc tcgtggtccg gggccagcag gcggctgatc agggtagtac
      781 gcaagtacgc attgctccgc cggaagtgcg tgcggcgaat cccgcatttg acgtgacccc
      841 agggcgcctt gtgacgggca tcattaccga cttgggcatt gcgatcccga cgcgcagggg
      901 actggacaag ctgtatcgga ccagagcggc ctgatcgtga ccgaattgca actcaggaaa
      961 gagaccatcg ccactgcgct gcatatgaac gcgctgggca ttaatcgtgg caaatccggc
     1021 aacgtgagcg cgcgctggaa gcacgggttt ctggtgacgc cttctgcgtt gccttatgaa
     1081 gaaatgcggc cgtcagatat cgtattcatc gataccaaag gccgatccaa ggggattcgc
     1141 aaaccgtcct cggagtggcg ctttcatttc gacatttacc ggcacaagcc aacctgccaa
     1201 tcggtagtac atacgcattc cagcttcgcg acaacactcg cttgcctggg catgggagtg
     1261 ccagccttcc actatatggt ggcggttgca ggcggcaaag acatccgttg cgcgccttat
     1321 gcgaccttcg gcacgcaggc gctatccgac catgccatcg cggcgcttga gcaacgcgcg
     1381 gcctgcctgc tcgctaacca cggcatgatt gccaccggca gcagtttgaa aaatgcattg
     1441 gcgctggcgg tggaagtcga ggcgctgtgc gaacagtact ggcgcgcatt gcagatcagt
     1501 acgcctcgct tgctgagcga ccaagaaatg gcgctggtcg tggaaaaatt tgtggcctat
     1561 aactcgctga gccattgaac ggacttgcgc ctcacttcac ttggaactag cttagtcaca
     1621 tgactgaaaa acccaacagc gctccgacct ccgcagaccc cacggaactt gcgcgcacgt
     1681 tcgcagagat tgcgctgcgc agcaaacatc tggtcgaaac gcacctcgct gcgcatgccg
     1741 gtcaggtagg cgaagaggcc agcgacgaac tcggtgtggg caaggccttc tcggaactgg
     1801 ccggccggct gatgagcgat ccattcaaac tggccgagat gtcgatgcgc atgtggcatg
     1861 actatttcaa cctctggcag aacactctgc tcaaggcgtc cggcgcagaa gcaccgtcgg
     1921 tggctcagcc cggcaagtcc gacacccgct tcagaagcga actatgggaa aacaatctgc
     1981 tattcgatta catcaagcag tcgtatctca ttgccgcgca gaacatccag aaatcggtgg
     2041 cggaagttga tggactggat ccggttaccg cgcgcaaggt gaaattcttt acgcgacagt
     2101 atatcgatgc gctggcaccg accaacttcg ttctgaccaa cccggaagta ctgaaaacaa
     2161 cgatcgaaac tggcggcaag aacctgcttg atggcctgaa ccacctgctc gcagacatgg
     2221 accgcgggga tggcaaactt gccatcagca tgaccgatcc caaggcattc acgatgggcg
     2281 aaaacgtggc aagcacgcct ggcaaggtga tattccagaa tgatctgatg cagctgatcc
     2341 agtaccagcc cacgacgagc tcggtctaca agacgccctt attgatcgtg ccaccctgga
     2401 tcaacaaata ttacatcctg gatctgcgcg agaagaactc cttcatccgt tttgcgctgg
     2461 accagggcat caccgtgttt gtaatttcgt gggtcaatcc ggatagcagg cacgctggca
     2521 agaccttcga cgattatctg ctggagggcc cgcgtgcggc gatggacgcc atcgaaaagg
     2581 cgaccggaga gcatgacatc aacatgatcg gttattgcct gggcggcacg ctgacggcgt
     2641 gtctgctggc atggatggcg gccaaaggcg aaacccgcgt taaaagcgtg acattcttta
     2701 ccacgttgat cgatttttca gagcctggcg aactgggcgt tttcgtcgac gaggaagtcg
     2761 tcagcaaact cgaaaagaaa atggcggagc gcggctatct tgaaggttct gaaatggcat
     2821 cgacgttcaa tatgctgcgc gccaatgatt tgatctggtc gttcgtggtc aacaactatc
     2881 tgctcggcaa ggagccgttt ccgttcgact tgctgtactg gaattccgat gcgacgcgca
     2941 tgcccgcgaa gatgcatact ttttacctgc gcaacatgta catcaaaaac aaattgcgcg
     3001 aacccggcgg catcactctg gcaggcgagt ccattgacgt ttccaggatc aaattaccgg
     3061 cttatttcat ttccacgctc gaagaccata tcgcgccctg gaaatcgact tatctgagcg
     3121 cgcaactgct gacgggtccg gtgcgcttca cgctgggcgg ctctggccat atcgccggcg
     3181 tcgtcaaccc gccttcagcc aataaatatt ggtattggac caatgattca cttctcgaca
     3241 cggcggaggc ctggctggcc gccgcggaca aacgcccggg gtcctggtgg actgactggg
     3301 caagctggtt tgccgggttc ggtggcgaac tgatccccgc ccgccaaccg ggacaaggca
     3361 agctggcggt gatcgaagac gctcctggca gttatgccaa gttcaggctg gatcaggcac
     3421 cggccgaaca cgccggcatg cccgatgcgc cggcacgccg taaaacaccc cgcaacaaac
     3481 ccgccgccat tcgggacatc cgcaagccgt tccagcgttg acaaatgcca ttggcaaggt
     3541 agccttgcca agcggtcgct ttcaggcccc gaacacaaga ggccggttgg agaatcgggc
     3601 cagtcagacc gattccatca aaagtccccc gcaagagagg caggcaggtg tgaccgtgga
     3661 gtcggttcca gacaatggcg tgacctataa acacttacaa ccggggtggc aatcacggca
     3721 tcgcgttgtg ggcaccctga cgtaagggtg ttatgagtcg accctcatgc gcgaactcct
     3781 gagccgtttc cgccgcctgt tcctgttcgc cgcgctgttc agcctggtga tgaacctgtt
     3841 gttgctggtg ccggcactct acatgctgca gatttttgac cgggtcatcg ccggccgcag
     3901 caacgaaacg ctggttctgc tgacgatctc ggtcggggta gcgttaatca tgatgatggc
     3961 gctggatgtg ctacgcgcac gcctgctcgc agccggcggc ctgatgctcg acaactggct
     4021 gggtccgacc gtcgtacgca cgctgtacga cagggcgcgc cggcccggcg gtatggaacc
     4081 ggtaaacggt ttgtccgatg tcgcggcgtt gcgcggcttc ctgaccgggc cgggcgtgct
     4141 ggcgctggtg gatgcgccgt ggtttccgat ttacctgatc gtcattttca tattccatcc
     4201 gctgctgggc gcggtcgcca gcgccggcgc gctgtcgctg ctgagtttga cgatcatcaa
     4261 cgaaaggctt acgcgtccgc cactggaacg tctgcaatcg cagggccgcc gcgcctcgcg
     4321 cctgatcgac gccgggttgc gcaacgccga gatcgtcagc gcgctcggca tcgccgatga
     4381 cctgacccgg cgctggcaga tgcagaacga ccaggtgctc gacctgcagc accaggcaag
     4441 ccggcgcgca acggcaatga gcgggatgac gcgtttcctg cgccagttac tgcaggtcat
     4501 gatgctgggt gtcggcgcct acctggtaat cgatcggcac gtatccagcg gcgtcatgat
     4561 cgccgcgacc atcctgctgg ggcgcgcact cgcgccggtg gaaacgctaa tcgcgggatg
     4621 gaagaatctg gtcgatgcgc gttcggcgct gcggcgcctg gatgcactgc ttgccaacac
     4681 ggcccagcgc gagtcgacac aattgcccgc gcctgaaggg cgtctggtgt tcgaccgcgt
     4741 ggtttttggc gtgaaaggcc gcgaacgccc gatcatcgcc ggcatttcgc tggaactcgc
     4801 gtcaggagaa tcgctgggca tcgtcgggcc cagcgcgtcg ggcaagtcga cgctggcgcg
     4861 cctcgcggtc ggcgtttggg aaccgaccat gggtacggtg cgcatcgacg gctcggatct
     4921 cgcaaactgg ccgcgcgagc gcatcggtcc cttcgtcggc tatctgccgc aggacatcga
     4981 actgttcgcg ggcaccgtcg cggaaaacat cgcgcgcatg ggcgaagtcg attcagatgc
     5041 ggtggtcgaa gccgcgcagc aggcgcgcgc gcatgagctg atcctgcggc tccccgaagg
     5101 ctacgataca ccggtcggcg aaggggggac cctgctggcc gggggccagc gccagcgcat
     5161 cgggctcgca cgcgccctct accgccgtcc gcgcctggtc gttctcgacg agcccacggc
     5221 tcacctggac gcagaaggcg aggaggcgct cgtcgcggca ttgcgctacc tcaagcagca
     5281 gcacgctacc atcattctca tcacccaccg cccgtcgctt ctgcaaaacg ccgatataga
     5341 caaggtggcc gtgctgaaaa cgggcgcgct tgatttgttc ggcacgcgcg gcgaaatcat
     5401 gggcaaggtg acccgcggcg cgctgactgt tgccagccag atgggaaagg caagctgaca
     5461 tgaccacgac cgcgcagcgg ctgccgaagg acctgtacgc tctggaagcc cgccgactca
     5521 tcggtctcgg gctcacggtg ctggtcgtgc tggtaatcag cataggactg tggatggtaa
     5581 ttacgccgct gaccggcgcg gccatcgcgc cgggcgcggt caaggtggac atgaaccgca
     5641 agacgctcca gcaccaggaa ggcggcatca tcaaggagat cctggtgcgt gacggcagtc
     5701 atgtcgaggc tggcgagacc ttgctggtga tcaacgacgt ccgcgtcgac gccaactacg
     5761 acctgctgat cacccagctg gattcggaaa tggccaaggc atcccgcctg ttcgccgaaa
     5821 gcacgctgga cgcgggcgtg gcgtacgcaa aggagttgga atcacgcagc gaggaatcac
     5881 gcgtcaagga gctcatgcgg cgcgaaaatg cggtgttcgc gactcgcagg cagatgctcg
     5941 atacccaaat caagctgctg aaggagcaga tccaggaagc caaggcggaa gccatcgctc
     6001 tgggcgagca ggtggaagcg gaaaagcgcg cagtcgcctg gcagcaggaa gaattgctgg
     6061 cgaaccagca gctggcggaa aagggattca tgagcaatac ccggttgctg gccctgcaac
     6121 gtggcgtggc cgactatgaa gcccgcctcg gcgagcatcg cgccaccctc gcgcaggcgc
     6181 gccagcgcga gggcgatttt gaactgcgga tcgcatcctt gcgcaacaac tacatgaagg
     6241 aagcggctga cgaactcaag gaggccaccg ccaagaccta cgacctgcgc gagcgtttgc
     6301 gcccttcgct cgacgctgcc cagcgtcagc gggtcattgc tccggtgtcg ggtgacgtcg
     6361 tcgatttgcg cgtcaccacc gtcggcgcgg tggtgggtcc gcgcgagtct ctgctcgata
     6421 tcgtgccggc aaatcccgag ctgatagtcg aggcgcgcgt tcgccccgag gacatcactt
     6481 acgtgaaaat cggcggcgac gcggatgtgc ggctgagcgc gttcaagcag cgcatcacgc
     6541 ccacagtgac gggcaaagtg acctatgtct cggccgacag tcttacggac aacaagaccg
     6601 gcgccaccta ctacctaacc cacgtcaaac tcacgcccga ggccctcagg gaagcgggag
     6661 acctcaaact ccaggctggc atgccggccg aggtattcgt caagactccg gcgcgcacgc
     6721 cgcttcagta tttgctcgat cccctgacgg gtttcctgca gcattccttc cgcgaacctt
     6781 gagccgggct tcctctccgc gagcggaatt agaggggacc gattgtgtat acaataattg
     6841 gccaataccc ggatcagtcg cggctgccga cgcgatgcaa ttctgcggga cccggttcac
     6901 aattccagaa acggcctggg gtgaaaatca tgaaactcag caaagaacaa agcgaacaat
     6961 ttgaccgcga cggctacctg tttttcccga gcctgttcac tgctcaggag gtcaaggtcc
     7021 tcctggatga agtaccagcg atctatgcac agcggcgtgc ggagaatgtc cgcgaaaaag
     7081 acagcgacgc ggtgcgcacc aatttcgccg cacacatgta taaccgccgg ttcgcgaaac
     7141 tcgcccgcca tccgcggatg gtcgagccga tcatgcagct tttcggcgaa gcggtataca
     7201 tgcatcagtt caaggtcaac gggaagatgg ctttcgatgg ggacgtgtgg caatggcacc
     7261 aggattacgg cacctggaaa aacgacgatc agatgccgga accgcgtgcc atgaatgtcg
     7321 cgatcttcct gggcgaagtc aatgagttca acggcccgct gatgttcatc cccggcagcc
     7381 acaagcttgg cgtgctggaa gccgaacacg atacttccac caccagctat ccgttgtgga
     7441 cgattaatca cgatacgatc cgcaagctgg tggaacgcgg cgggctggtc gctcccaaag
     7501 gtccagcggg atcgatgatc ttgtttcatt cttgcctggt gcacgcatcg aactgtaatt
     7561 tgtcgccctg ggatcgcatc aacgtttatt tgagcctgtg cgcggtgtcc aaccacatcc
     7621 gccggtttaa acgccccgag tacatcgccc atcgtgattt cacgcctatc cactgcctgc
     7681 ccgatgattg cctgatcaag gactacgacg tggcgctgcc ctggaaagac ggaacgcccg
     7741 cggaggcgct caagccgctg gctgcgtaat cagcaacatg aacctgtttg ccaaacttcg
     7801 tgagcgagaa gcacagggaa agccactgcg ggtaggtctg atcggggccg gcaaatttgg
     7861 cgccatgtat ctcgcccagg taccgaaaac gccgggtgtg catttgagcg gcattgccga
     7921 cctgtcccct gcgaacgcga aggcaagctt gcaacgggtc ggctggaaag ccgaacgcta
     7981 tgcggcgccc acattggatg cggcattctc ttccggcctg actcatgtca gcgacgattg
     8041 gcaagcactg gtggagcatc cgctgatcga tattatcgtc gaggcaacag ggaatccgat
     8101 agccgcggtg gaacatgctt tggccgcatt caaacacggc aagcacgttg tcatggtcac
     8161 ggtcgaggcg gatgcgatgt gcggaccctt gcttgcgcgt cgcgccagga acgccggtgt
     8221 ggtctacagt cttgcctacg gcgatcagcc ggcactgatc tgcgaccttg tcgattgggc
     8281 gcgcgcgtcc gggttcagtg tggttgccgc cggacgcggc cataaatggc tgccgcattt
     8341 cgcgcactcg acaccagaaa cggtgtgggg ttattacggt ttgacgcccg aacaggcgaa
     8401 aacgggcgga ctcaacccga aaatgttcaa ttcgtttctg gatggttcca aaccagccat
     8461 cgaatgcacg gcggtgtgta acgccaccgg gctcacaccc gctccttacg gcttgctgtt
     8521 tccgcccgcc agcgtcgatg acattcccaa tgtgatgcgt ccgatagccg agggcggcca
     8581 tttgcaccat aaaggccagg tcgaggtcat ttcttctctc gagaccgacg gtcgggtcat
     8641 tccttacgat attcgcttcg gcgtattcgt ggtgttcgaa ggcgagaccg aatacattcg
     8701 caactgtttc aaggaataca tggtgcgcac tgatgcatcg gggcgttatg cgtgcctgta
     8761 caaaaggtgg catttgatcg gcctggaagt cggcatttcg gtggcatcgg tcgggctgcg
     8821 cggcgaaccc accggttgcc cgaccggttt ccgggccgac gtggtggcaa ctgcgaagcg
     8881 caacctgaag gccggtgaaa tccttgacgg tgaaggcgga tatacggtcg tcggtaaact
     8941 gatgccggcg gacacgtcta taactatcgg cgccttatcc ctcggccttg cgcatggggt
     9001 gaaaatgctg cggcc
//
LOCUS       DATMZQ010000078         3780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_62408, whole genome shotgun sequence.
ACCESSION   DATMZQ010000078 DATMZQ010000000
VERSION     DATMZQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62408"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VN496_02515"
     CDS             complement(<1..860)
                     /locus_tag="VN496_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXF15845.1"
                     /translation="MLARLDPGSASARRGLSDIAIRNLFIIPTISFLIVFNIFPLLYS
                     LGYSFTDFRASTSSPAHFIGLENYRELLADPYIWTNFTITAKYVLVSVGGQVLVGFGV
                     ALLLNRKVPAKGLVTTLLLLPMMMSMAVVGLFWQLLYSPSWGIINYALGLEDFEWLSN
                     ADIALYAVALTDIWMWSPFVMLLSLAGLSAIPQHLYEAAAIDRATRWYTFTRITLPLV
                     TPLLLIAIIFRTMEAFKTFDLAFIMTSMDTTELIAIRLYKMAFQEWQTGKSCALAYIV
                     LIMVLAITNI"
     gene            complement(948..2261)
                     /locus_tag="VN496_02520"
     CDS             complement(948..2261)
                     /locus_tag="VN496_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXF15846.1"
                     /translation="MSKKTWSGLLAGAAFALACSSALAAEKLTILWAQWDPANYLQEL
                     VKDYEKQTGVKVTVETTPWPDFQTKAFREFAAKGDAYDLVVGDSQWLGAGSTAGHYVD
                     LTDFFKKHGVDKSMAPATVEGYAEYPKGSKKYWAVPLEGDATGWSYRKDWFEDPKEMA
                     AFKKKYGYDLGVPKTWAHMRDIAEFFHRPDQKRYGIAIYTDNSYDALVMGYENVLFGY
                     GGELGDYSSYKVKGVIDSKTAIDALKFYKELYKYTPPGWGKVFFMEDNQAITEGLAAM
                     SMNFFAFFPALTNPATNKNAKVTGYFSNPAGPSGKRFAALGGQGISIISYSKKQAEAT
                     KFLEWFIKDDVQQKWAELGGYTCSAKVLQSEKFRKATPYNQAFYETMFMVKDFWAVPE
                     YAELLDSMNKRLHPFIVGNKGTAEEALTGVANDWDKTFRKAGLLK"
     gene            complement(2376..3014)
                     /locus_tag="VN496_02525"
     CDS             complement(2376..3014)
                     /locus_tag="VN496_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXF15847.1"
                     /translation="MLEASMRLMQDGHIPSVSDVAAAAQVSRATAYRYFPTQAALIQA
                     AVNEALGPILAWTSDSSDAEERVTQLVTFAYPRMEDYEATLKAALRLALDQWARRHAG
                     LAGSEEQMVRGNRIGLLHSAIQPLKSKLTAAGFARLKQSLSLVFGTEAFVVLKDIWGL
                     DGHRAQNVALWTCHALIQATIAENGKAGSHSGRSSRHRASTTLRNSVARKGK"
     gene            3314..>3780
                     /locus_tag="VN496_02530"
     CDS             3314..>3780
                     /locus_tag="VN496_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HXF15848.1"
                     /translation="MKRIYVVGTADTKGLELEYVRSLVKAAGTPAIIVDVGTRTAQIK
                     TGVAATEVASFHPRGKEFVLSSEDRGTAVEAMAEAFARYIVTRTDIAGVIGIGGGGGT
                     SIITAGMRKLPVGLPKLMVSTLAAGDIGQFVGANDITMMYSVTDVAGLNRISRV"
BASE COUNT          831 a         1130 c         1008 g          811 t
ORIGIN      
        1 taaatattcg tgatggcaag caccatgatc aacacgatgt aggccagcgc gcacgatttg
       61 ccggtctgcc attcctgaaa cgccatcttg taaagacgaa tggcgatcag ttccgtggtg
      121 tccatgctgg tcatgataaa agccaggtcg aaagtcttga atgcctccat ggtgcgaaaa
      181 ataatggcga tcaacagcaa gggtgtcacc aacggcagcg tgatgcgggt aaaggtatac
      241 cagcgtgtcg cgcgatcgat cgctgcagcc tcatagagat gctgcggaat cgccgacaac
      301 cccgccagcg agagcagcat gacaaacggc gaccacatcc agatatctgt cagcgcaacc
      361 gcatacagtg cgatgtcggc attcgacagc cattcgaaat cttccagccc gagcgcgtaa
      421 ttgatgatgc cccatgaagg gctgtagagc agttgccaga acagcccgac gaccgccatt
      481 gacatcatca tcggcagcaa cagcaacgtg gttaccaggc ctttggcggg cactttgcga
      541 ttgagcagca gggccacgcc aaagccgacc agcacctgcc caccgaccga caccagcacg
      601 tatttggcgg tgatggtgaa attggtccag atgtatggat cggcaagcaa ctcgcgataa
      661 ttttcgagac caataaagtg cgctggcgac gaagtggatg cgcgaaaatc cgtgaaagag
      721 tagccgagcg aatacagcaa cggaaatatg ttgaagacga tcagaaaact gatcgtcgga
      781 atgatgaaca ggttgcgtat ggcgatatca ctcaggcccc gcctggccga agcggagcca
      841 ggatcaagcc tggccaacat ctaatttcct cccgccggtt ggacgaatcc gtgctcctca
      901 ccgcaaacac gggaaggcgt cccgccttcc cgtccaaacc aaaaaagcta cttgagcaag
      961 cccgccttgc ggaaggtctt gtcccagtca ttggctacgc cggtcaatgc ctcttctgcc
     1021 gtgcccttgt tgccgacaat gaaaggatgc agacgcttgt tcatggaatc gagcagctcc
     1081 gcgtattccg gcaccgccca aaaatccttg accatgaaca tggtctcgta gaaggcctgg
     1141 ttgtacggcg tagccttgcg gaatttttcg gattgcagga ctttcgcgct acaggtatag
     1201 ccaccgagtt ccgcccactt ctgctgcacg tcatccttga tgaaccattc caggaacttg
     1261 gtggcttcgg cctgcttctt ggaataactg atgatggaaa tgccctgtcc gcccaaggcc
     1321 gcaaagcgct tgccgctggg accggcagga ttggagaaat agccggtgac ttttgcattc
     1381 ttattggtcg caggattcgt cagcgccggg aagaaggcaa aaaagttcat gctcatcgcc
     1441 gccagccctt cggtgatggc ctggttgtct tccatgaaga acaccttgcc ccagccgggt
     1501 ggagtgtact tatagagttc cttgtagaac ttgagtgcgt cgattgcagt cttgctgtcg
     1561 atgacgcctt taactttgta actgctgtaa tcaccgagtt cgccgccgta accgaatagc
     1621 acgttttcgt agcccatcac cagcgcgtca tagctattgt cggtgtagat tgcaatgccg
     1681 tagcgtttct gatccggacg atgaaagaat tcggcaatgt cacgcatgtg cgcccaggtc
     1741 ttcggaacac cgaggtcata gccatacttt ttcttgaaag cggccatttc cttgggatct
     1801 tcgaaccagt ccttgcgata cgaccatcct gttgcgtcgc cctcaagcgg cacggcccag
     1861 tatttcttgc tgccctttgg atactccgca tagccctcta cagtagcggg cgccatgctc
     1921 ttgtccacgc cgtgcttctt gaagaagtcg gtcaaatcca cgtagtgccc ggcagtggaa
     1981 cccgcgccga gccactgcga atcgccgacc accagatcgt atgcatcgcc ttttgcagca
     2041 aactcacgaa acgccttggt ttggaaatcc ggccagggcg tcgtctctac cgtgaccttt
     2101 acgccggttt gcttttcgta gtccttcacc agctcctgca agtaattcgc cgggtcccat
     2161 tgggcccaca ggatggtcag tttttccgcc gcgagcgccg aactgcaggc cagggcgaaa
     2221 gcagcgccgg ccagcaggcc ggaccaggtc ttcttgctca ttgcatgctc ctccacattg
     2281 gtaatttttt aagccggaca ttgtgactaa aacagccggc caccccagaa acccgccgca
     2341 caactctgcc gcggccctaa cagaacgctc cgttcttact tacctttccg tgcaacactg
     2401 ttcctcaatg tcgtgcttgc cctgtgacgc gacgatcgcc cactatgact gcctgcctta
     2461 ccgttctccg cgattgtggc ctggatgaga gcgtgacaag tccacagggc aacattctgc
     2521 gcacgatgac catcgagacc ccaaatatcc ttcaatacca cgaacgcctc cgttccaaac
     2581 accagcgata gcgactgctt caatcttgca aagccggccg cggtcagctt gctcttcagc
     2641 ggctggatcg ccgaatgcag caggccaatg cggtttcccc gcaccatctg ttcctcgcta
     2701 ccggcgaggc cggcgtgcct gcgcgcccac tggtccagcg caagacgcag tgctgccttt
     2761 agcgtcgcct catagtcctc catgcgcgga taagcgaagg tcaccagctg cgtgaccctt
     2821 tcctctgcat ccgaagaatc ggaagtccac gccaaaatcg ggccgagcgc ctcattgacc
     2881 gcagcctgga tgagcgcggc ctgagtggga aaatagcggt acgcggtcgc ccgagaaact
     2941 tgcgcggcag ccgccacgtc gctgaccgaa gggatgtgtc catcctgcat caagcgcatg
     3001 gaagcttcca gcatgacctt gcgcatccgc gcgcgcggac ctctttccgc ttgtactttt
     3061 cctcccggtt caattttgtt gttagcgcgg cgcttgagac gccgttcttg acctgagact
     3121 agcatctcac tacgatacca gcgtattacc caaaaaaaaa tttcgccagc gtttattttc
     3181 gcgctgcgac gcacaaagcg cgatagccgc gcatcattcg gaggagaaaa cgcatgctgc
     3241 gtgacggaga gggcgcgctg cgtttgcgtc tacggcaccg tccttccccg cttcattcct
     3301 gtgcaccaac gcgatgaagc gcatatacgt ggtcggcacc gctgatacca aaggcctgga
     3361 gctggagtat gtccgatcgc tggtcaaggc ggccggcact cccgcgatca ttgtcgatgt
     3421 cggtaccaga accgcccaga ttaaaaccgg cgttgcagca actgaagtcg catcgttcca
     3481 tcccagaggc aaggaattcg tgctcagcag cgaggatcgc ggcaccgctg ttgaggcgat
     3541 ggcagaagcc ttcgcacgat acatcgtaac ccgcaccgat atcgcgggcg taatcggcat
     3601 cggtgggggc ggcggcactt ccatcatcac tgccggcatg cgcaagttgc cggtcggcct
     3661 tccgaagctc atggtttcca ccctcgctgc tggcgatatc ggccagttcg tgggtgccaa
     3721 cgacatcacc atgatgtatt cagtgaccga tgttgccggt ttgaaccgca ttagccgagt
//
LOCUS       DATMZQ010000079         5150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1790467, whole genome shotgun sequence.
ACCESSION   DATMZQ010000079 DATMZQ010000000
VERSION     DATMZQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790467"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VN496_02535"
     CDS             complement(<1..554)
                     /locus_tag="VN496_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXF15849.1"
                     /translation="MLGVLSAALLIGCATKAPPTPEQVRQEAMKNIALPGAWKAGADA
                     GPIADNWLAALGDPQLDALIAEAMTNNPELRVTAVRVAQSAQYVELAKAALRPSVNLL
                     GTGGINMGGGDALQFISLGVSWEADLWGRMRYSRNAYQESYASAQADFEFGRQSLAAS
                     VAKGWFTASETWLQLKIAEDMVKAS"
     gene            complement(579..1760)
                     /locus_tag="VN496_02540"
     CDS             complement(579..1760)
                     /locus_tag="VN496_02540"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HXF15850.1"
                     /translation="MDILLLGIYSFFVWLIFIKFKWLPWNIVSQVIVITIPVVALTIL
                     ILVLNVVAPSSADVRVVNYVVPVNPRVSGLVTEVPIEPNRPIKKGDILFKLDSTPYAL
                     EVQGFEAQINQLEVQLITAGANQRGLGEELKAATGKKASTRSKLALTRQRLKQYKELA
                     EAGAGNRFDYEQALADTANLEAELASATASESQIREKLLARTPSGEQDEIANVKAQIM
                     RTEAQLADAQWKLEQTVYRAPADGTVVSLALRPGAMAVQFPAMPAMTFVENEQWIMAI
                     FRQNEVRRVEPGQEAEIAISTLPGRIIKCKVDSIMWATAQGQLPIGGASSSAGIAPIP
                     PGHLAVRLHLDDKDKDVFLPSGATGNGTIYTDAGEAIHILRKVFMRVGTKLDWLVLKL
                     H"
     gene            complement(1764..2285)
                     /locus_tag="VN496_02545"
     CDS             complement(1764..2285)
                     /locus_tag="VN496_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HXF15851.1"
                     /translation="MKRLLESRHARAFLQRAPGIFLALAAWIPYSAHASLFKGETLDM
                     VANAIAWVVLVVVPIVVIVVFWLVHILPEKIAEKKHHPQAKAIQVLCLLSLAFGGLLW
                     PLAWLWAYSKPVLYKMAYGTDRVVHGEEVLALPSNEETEADELREIRRLRRRVAELEN
                     SLARQPISRVEKG"
     gene            complement(2441..3886)
                     /locus_tag="VN496_02550"
     CDS             complement(2441..3886)
                     /locus_tag="VN496_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HXF15852.1"
                     /translation="MNISDRRIRVASANAVKGWSALVIAWPVLWGCAQAPPPMPVPQA
                     PKAVVSYVNIPIAIELDAIEKAAETALQRSIGVQPFQQALNGGSNAPSCGEDVGYSIE
                     RGPIALSGAGTVLTTTLDLSYWLKARKQLPCPGAAINAWCGTDGEPLRTARVAIDSEI
                     TILPNLATSVHSKLQRATAGNPCVMQPVGVDITESLMVAFGTTLGQILPALDTRMSAE
                     LDLRTPIEAAWQRMSEPREVRPAVWLVWNPEALGIVPVTVRDGVLRTGLQLRVRPVIA
                     TGAKVASNPKPLPLAYGATHDDSFRLQIPVEVDESFIQANLDKALNIDQGGMEVTIGN
                     YKIRITSADITGEGSQVLIKARFSGDFSGSFDLAGTPYLNPETQVLNFPDLDYTLDSD
                     QVLLQSASFVAHSQIRDRLREQYTIPLSDRISQFKGQIDSLLNRRNGNVQLHGGVDDL
                     NLLGISRPANGSVFTVYLAARGKVTAEILAP"
     gene            4332..4889
                     /locus_tag="VN496_02555"
     CDS             4332..4889
                     /locus_tag="VN496_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15853.1"
                     /translation="MKKIVVFAALISGLSMISPAGAEQKAGVVVADSTETIVTVVDVD
                     REARTVTVRGEKGHLTMINVPERAQNLDQVVPGSRFKVQYVRSVALSLRKGSGVASSN
                     TGSTVELAAKGDTPGGTMVSVHQVNAVVEALDRDSRSVTLRGPEGGPFDVQVGEEVKA
                     YGDIEVGDTVTVEYTEGLAMHMIKQ"
BASE COUNT         1033 a         1501 c         1533 g         1083 t
ORIGIN      
        1 gaggccttga ccatgtcttc ggcaatcttc agttgcagcc atgtctcgct tgcggtgaac
       61 cagcctttgg ccacagacgc tgccagcgat tgccgtccga actcgaagtc agcctgtgct
      121 gaggcatacg actcctgata agcattgcgg ctgtagcgca tgcggcccca caagtccgcc
      181 tcccacgaaa cgccgaggga aatgaattgc aaggcatcgc cgccgcccat gttgatgccg
      241 ccggtgccaa gcaaattcac gctgggacga agtgcagcct tggcaagctc gacgtattgc
      301 gcggattgcg caacccgtac tgcggttacg cgcagttcag gattgttggt cattgcttcc
      361 gcgatcagtg catcgagctg cgggtcaccc aatgcggcaa gccagttatc cgctattgga
      421 ccggcatccg ccccggcctt ccatgcacca ggcagcgcaa tatttttcat tgcttcctgc
      481 cgcacctgtt cgggggtcgg cggcgccttg gtggcgcaac cgatcagcag ggcggcgctc
      541 agcacaccga gcatccattt cacggcttgc atctcccgtt agtgcagctt cagaaccagc
      601 cagtcgagct tcgtcccgac acgcatgaag actttgcgca gaatatgaat cgcctcgccg
      661 gcgtcggtat agatcgtgcc gttgccggtc gcgcccgacg gcaggaatac atctttgtcc
      721 ttgtcatcca gatgcaagcg gacggcaaga tggccgggcg gaatgggcgc aatgccggcg
      781 ctgctgctgg ccccgccgat aggcagttgt ccctgcgccg tggcccacat aatggaatcg
      841 actttgcact tgatgatgcg gccgggaagc gtcgatatgg caatttcagc ttcctggccg
      901 ggctcgaccc gccggacttc gttctgtcgg aagatcgcca tgatccattg ctcattctcg
      961 acgaacgtca tggcaggcat cgcggggaac tgtacggcca tcgcgccggg ccgcagggca
     1021 agcgatacta ctgtgccatc cgccggagcg cgatatacgg tttgctcaag tttccattgc
     1081 gcgtcggcga gctgcgcctc cgtgcgcata atctgtgcct tgacgttggc aatttcgtcc
     1141 tgttcgccag aaggcgtcct ggccagcaac ttttcacgga tctgcgattc acttgcggtg
     1201 gccgaagcga gttcggcttc gagattggcc gtatccgcca gcgcctgttc gtagtcgaaa
     1261 cgattgcccg cacccgcttc ggcaagttcc ttgtactgct tcaggcgctg gcgcgtgagg
     1321 gcgagcttgg aacgtgtcga ggctttcttt ccggtagcgg ccttcagttc ttcaccaagg
     1381 ccgcgttgat tggctcccgc ggtgatgagc tgtacttcca actggttgat ctgcgcttca
     1441 aagccctgga cctccagcgc atacggcgta ctgtcgagtt taaacagtat gtcgcccttc
     1501 ttgatcgggc ggttcggttc gatgggtact tcagtgacaa gcccgctgac tcgcggattt
     1561 accggcacca cgtagttgac gacccgcacg tcggcagagg agggggccac gacgttcaag
     1621 accagaatca atatggtcag cgcgacgacc ggtatggtga tcacgatgac ctggctgacg
     1681 atattccagg gaagccattt gaatttgatg aagatcagcc agacgaaaaa cgagtaaatc
     1741 ccaagcagga ggatgtccac tacttagccc ttctccaccc ggctgatcgg ctgccttgcc
     1801 agactgtttt cgagttcggc cacgcgtctg cgaagtcttc tgatctctct cagctcatcc
     1861 gcttccgttt cttcgttcga tggcagagcg agaacctctt cgccatgcac tacgcggtcg
     1921 gttccgtaag ccatcttgta gagcaccggt ttggagtacg cccatagcca ggcgagcggc
     1981 cagagcagtc caccgaacgc gagcgacagc aggcaaagta cctggatggc cttggcttgc
     2041 ggatggtgct tcttctcagc aattttttcc ggaaggatgt gaaccagcca gaaaacaaca
     2101 atgactacaa tgggcacgac aacgagcacg acccaggcga ttgcattggc caccatgtcc
     2161 agcgtttccc ctttgaatag cgaggcgtgc gcggaatagg gaatccatgc cgccaaagcc
     2221 agaaaaatac caggggctcg ttgaaggaat gcgcgcgcgt gtctggattc cagcaaccgt
     2281 ttcatattga gatcgcgata ttcatagtca gcgcatgatc ctacatgcct tctgcaaatt
     2341 agagaacctt tcgatacctt cgtcggttcg ctggctggaa ccggtcctga ggctatccct
     2401 tgccgggacc gggcgccaca cgggttcggc gtccagatct tcacggcgca aggatttcgg
     2461 cggtcacttt gccgcgggcg gcgagataca cggtgaatac tgagccgtta gccggcctcg
     2521 agatgcccag caagttcaag tcgtcaacac cgccatgcaa ctgcacgtta ccgttgcggc
     2581 gatttagcag gctgtcgatc tgccccttga actgcgaaat acggtcgctt agcggaatgg
     2641 tgtattgctc ccgcaggcgg tcgcggatct ggctgtgtgc aacgaaactc gcagactgca
     2701 gcaggacctg gtccgaatcc agcgtgtagt cgaggtccgg aaagttgagt acctgtgtct
     2761 cgggattcag ataaggcgta cccgcgagat cgaaactccc gctgaagtct ccgctgaagc
     2821 gtgccttgat gagcacctgc gagccttcgc ccgtgatgtc tgcgctggtg atgcggatct
     2881 tgtagtttcc gattgtgact tccatcccgc cctggtcgat attcagtgcc ttatccagat
     2941 tggcctggat gaatgattcg tcgacctcga ccgggatttg caatctgaag ctgtcgtcgt
     3001 gcgtggcgcc gtacgcaagc ggaagcggct tcggattgct cgcaaccttt gcgcctgtcg
     3061 cgatgactgg ccttacgcgt aactgcagac ccgtacgcag tacgccgtcg cgcacggtta
     3121 ccggcacgat accaagcgcc tccggattcc agaccagcca cacggccggg cgtacctcgc
     3181 gcggttcact catcctttgc caggccgcct cgattggggt gcgcaggtcc agttcagccg
     3241 acatgcgtgt atccagtgcg ggcaatatct ggcccaatgt cgtaccgaag gcaaccatga
     3301 gtgattccgt gatgtcgacg ccgacgggtt gcatgacgca cgggttacct gcggtcgcgc
     3361 gttgcaattt ggaatgcacg ctcgtcgcca ggttggggag gatggtaatc tcgctgtcga
     3421 tggcaacccg ggcggtccgc aagggctctc cgtcggtccc gcaccaggcg ttgattgctg
     3481 cgccgggaca aggcaattgc ttgcgggctt tcagccaata actcaagtcg agcgtcgtgg
     3541 taagcaccgt gcccgctccg gagagggcga tgggaccacg ctcgatggag tagccgacgt
     3601 cttccccaca agatggagcg ttcgacccgc cattgagcgc ttgctggaac ggctggaccc
     3661 caatggagcg ctgcagggcc gtctcggccg ccttctcaat ggcatcgagc tcaatcgcaa
     3721 tcggaatatt gacgtacgag acgacggcct tgggtgcctg gggtaccggc atgggcggcg
     3781 gcgcctgggc gcaaccccaa aggaccggcc acgcaatgac cagagcggac cagcccttca
     3841 ctgcgttcgc actcgccacc cgaatccggc ggtcggatat gttcactgca aaatactcca
     3901 caaacgtgtg aaagcgccac aaacctgatg gaccagggcc gcgtggcgat gggtaacccg
     3961 gaaaaatggt ctcgggatat aagttagagc aagggctgca ttgtcgggca tttgccactg
     4021 tccatgtgca gcctagtgta ttggcacttc ctctgcccgg tttgatctgc gtcaaaccgg
     4081 cgatttttgc cgcgatgccg agggaacgcg cacacagatg gcagccgtat agtgttagca
     4141 tcgaccaaaa taaacaaacc tgtgctaaag aggttcagcc aatgcccacg gtattggtac
     4201 cacgtcgcga aattgcaagc cgcattgcat tgcaaggccc gggagcatag cgccccggaa
     4261 caaatcgttt ttagtttcgg tgcgccgccc tggcgtgccg gcctgttcga taaccaagag
     4321 ggggatttga gatgaaaaaa atagttgtct tcgccgcact gatttccggg ctgagcatga
     4381 tcagtccagc cggtgcggaa caaaaagcag gtgtggtcgt ggccgactcc acggagacga
     4441 tcgtcacagt ggttgatgtg gaccgggaag cacgtaccgt cacggtgcgt ggagaaaaag
     4501 ggcacttgac catgattaac gtcccggagc gagcgcagaa cctggatcag gtcgtgcccg
     4561 gctcgcgctt caaggttcag tacgtgagat ccgttgcgct gtcgctgcgc aaaggcagcg
     4621 gcgttgccag ttcgaacaca ggcagcacgg tcgagctcgc cgccaaagga gacactcccg
     4681 ggggcaccat ggtcagcgtg caccaggtca acgcggtggt agaggcgctg gatcgcgata
     4741 gtcgttcagt cacgctgcgc ggaccggaag gcggcccatt cgatgtgcaa gttggcgaag
     4801 aggtcaaggc ttacggcgac attgaagtcg gcgacaccgt taccgttgag tataccgaag
     4861 ggttggcgat gcacatgatt aagcagtagc acgcacggcg gctatgaccg tttgcatcta
     4921 agccgccgtc ccgcacgccg cgaactgcag tcccggctga atcctgatca gatccggcat
     4981 caatatgcgc gtccgtactg tgctgcgtcg tatcctgatt gcgggagcaa tagtcctggt
     5041 gctgttggca gcgatcctgc tcagtgctcc gctattgctc tccttgcctt cactgcacga
     5101 ccagctggag caaaaagttt ccgaggcggc tggcggccag atcacctggg
//
LOCUS       DATMZQ010000080         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2104673, whole genome shotgun sequence.
ACCESSION   DATMZQ010000080 DATMZQ010000000
VERSION     DATMZQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104673"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="VN496_02560"
     CDS             complement(<1..1685)
                     /locus_tag="VN496_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXF15854.1"
                     /translation="MQQIKNKQGTAALALGALGVVYGDIGTSPLYTIKETFGPNTGVA
                     LNPANLIGAASVIVWALMLVVTLKYVLLILRADNRGEGGIIALTALAVAAAGKTPRWR
                     STLILIGIAGAALFYGDSVITPAISVLSAVEGLHVATPALDPYVLPVSVGVLVVLFAV
                     QNRGTASVGVLFGPVIVLWFAVLAVAGVVQILREPAILAALNPVRAYDFLAARGWGVF
                     AAIGAIVLALTGAEALYADLGHFGRKPIQLAWCGFVLPALALNYLGQGALLMADPSAL
                     ENPFYRLFPPVLLLPAVVLATSATVIASQAVISGAYSMTRQAIQLGVLPRMRIIHTSA
                     REIGQIYMPAINWTLLAAVLVATIGFGSSSALASAYGIAVTLTMFITTLLTFFVVRHA
                     WRYPLWVAIGATGFFLVVDGVLAVSCLLKFSQGGWFPVLIAALILVLMTTWMRGRNLL
                     LATMRADDPPLVPFLQGLLQSQPPQAARTAVFLTADPETVPHALLHNLKHNNVLHRTN
                     VILTVVFHELPWIESKDRVQAQQLSPLFWRVLVNYGFRDTPDVPQALGSCAVQG"
     gene            complement(1755..>2405)
                     /locus_tag="VN496_02565"
     CDS             complement(1755..>2405)
                     /locus_tag="VN496_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF15855.1"
                     /translation="DDAEIRLFLKTSLVADGFAVFTAAGGREALQLSLHRSFALIVLD
                     LGLPDMDGVEVVRDIRSKSAVPILILSARVDERRKIEALDAGANDYITKPFGIGELRA
                     RIRVALRLAGGVQAPAYELDRLRIDFARHRVRYANRDVHLTPIEYQLLARLAHSAGNV
                     VTHRQLLQDVWGPEYVEHTHYLRIYMGQLRAKIESDPADPRFLLTETGVGYRLADE"
BASE COUNT          560 a          725 c          699 g          421 t
ORIGIN      
        1 ccctgcactg cgcaagaacc aagtgcctgc ggaacatccg gcgtatcgcg gaagccatag
       61 ttcaccagga cccgccagaa caacggcgaa agctgctgcg cctgcacacg gtccttggat
      121 tcgatccatg gcagctcatg aaacacgacg gtcaggatta cgttcgtccg gtgcagcaca
      181 ttgttgtgtt tgaggttatg cagcaaggca tgcggtacgg tctcgggatc cgcagtcagg
      241 aagaccgccg tgcgggccgc ctgcggcggc tggctttgca ggagtccctg caaaaacggt
      301 acgagcggcg ggtcatcggc gcgcatggtg gcgagcagca aattgcgccc acgcatccag
      361 gttgtcatca ataccagaat gagcgcggcg atgagcactg gaaaccagcc gccctgcgaa
      421 aatttcaaca ggcaggacac ggctagtacg ccatcgacga cgagaaagaa tccggttgca
      481 ccgattgcca cccataatgg ataacgccat gcatggcgaa ccacaaaaaa ggtcagtaat
      541 gttgtgatga acatggtcag cgttaccgcg atcccgtagg ccgaggccaa cgcagacgat
      601 gatccgaagc cgatggttgc aaccagcacg gcggccagta gcgtccagtt gatggctggc
      661 atgtagattt gcccgatctc gcgggctgac gtatggatga tacgcatgcg cggcagcacg
      721 ccaagctgaa tggcttggcg cgtcatggaa tacgcgcccg aaatgactgc ttgtgatgcg
      781 atgacagtag cgctggtagc cagtacgacg gctggcagca gcagtaccgg aggaaacagt
      841 cgatagaatg ggttttcgag cgcggacggg tccgccatca atagcgcgcc ctgaccgaga
      901 taattcaggg ccagcgccgg caggacaaac ccgcaccatg cgagttgtat cggctttctc
      961 ccgaaatgac ccagatccgc ataaagcgct tcggcacccg ttagggcaag cacgatggcg
     1021 ccgatggccg cgaacacccc ccagccgcgt gccgcgagaa aatcgtaagc gcgcacaggg
     1081 ttcaacgcgg ccaggatcgc cggttcgcgc aaaatctgga caaccccggc cacagccagc
     1141 acagcgaacc agagcacgat gaccgggccg aacagtaccc cgactgaagc cgtgccgcga
     1201 ttctgcacgg caaacaagac gaccagcacg ccaaccgaca caggcaacac atacggatcc
     1261 agcgccggcg tggcaacgtg caacccttcc accgcgctca aaaccgaaat cgcgggcgtg
     1321 atcacgctat ctccatagaa cagcgctgca ccggcgatgc cgatcaggat aagcgtgctg
     1381 cgccagcgcg gcgttttgcc ggcagcggca accgcgagtg cggtgagggc aataatgccc
     1441 ccttcgccac gattatccgc acgcaggatt aacaacacat atttcagcgt gacgaccagc
     1501 atgagtgccc aaacgatcac ggacgctgcg ccaatcaggt tggccggatt gagggccact
     1561 ccggtgttgg gaccgaaagt ctccttgatc gtgtacagcg ggctggtgcc tatgtcgcca
     1621 taaacaacgc ccagcgctcc gagcgcgagt gcagcggtgc cctgcttgtt tttgatctgc
     1681 tgcacaccaa cctccaccga atcgtgaggt tgctttttag cgtggactgc gtaaaaaaag
     1741 aataaagatc ccaatcattc gtcggcaagc cggtaaccaa ctccggtctc ggtcagcagg
     1801 aaacgcggat cagccgggtc gctttctatt tttgctcgca gctgtcccat gtagatacgc
     1861 aaataatgtg tgtgctccac atactcaggt ccccacacgt cctgcaatag ttgccgatgt
     1921 gtcacgacat tgccggcact gtgcgccagc cgcgccagca actggtactc gatcggcgtt
     1981 aagtgcacgt cccgattggc ataccggact cggtgtcgcg cgaaatctat gcgtaaacga
     2041 tccagctcgt aggccggcgc ttgcacgccg ccagccaggc gcaacgcaac tcgaatgcgc
     2101 gctcgcaatt ctccaatccc aaaaggcttg gtgatgtagt cgtttgcgcc ggcatcgagg
     2161 gcttcgatct tgcgccgttc atcgacgcgg gccgaaagaa tgagaatggg tacggctgat
     2221 ttgctgcgaa tgtcgcgcac cacttcgaca ccgtccatat ccggcaggcc gaggtcgagt
     2281 acgatcagcg cgaacgaacg atgcagtgac agttgaagcg cttcccgtcc ccctgccgca
     2341 gtgaacacgg cgaacccatc cgctacgagg ctggttttca ggaacaggcg aatctcggca
     2401 tcgtc
//
LOCUS       DATMZQ010000081         2487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1791316, whole genome shotgun sequence.
ACCESSION   DATMZQ010000081 DATMZQ010000000
VERSION     DATMZQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791316"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VN496_02570"
     CDS             <1..496
                     /locus_tag="VN496_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HXF15856.1"
                     /translation="RFMMDLRTRLVTGNIFQGDDVVQLSTDGFTCYPEAVDLAFGPHV
                     KFGTIVKEFRNAVRPYTPSEIVGSRRRGIFNISGREQTRTICTSHVERSNLTIRTFMR
                     RFTRLTLGFSKKLENLAAAFALHAAHYNFCRVHGTLGKTPAMAAGVTNDIWTLADLYE
                     KALG"
     gene            complement(536..778)
                     /locus_tag="VN496_02575"
     CDS             complement(536..778)
                     /locus_tag="VN496_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15857.1"
                     /translation="MGHFEFSFIEFASWGMMVGMENKLRLSIGAAFGMALGSLLAVAE
                     GRTSNTSDMLLFLILGAVLGSLCGVAFEAFERRKRR"
     gene            complement(903..1172)
                     /locus_tag="VN496_02580"
     CDS             complement(903..1172)
                     /locus_tag="VN496_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15858.1"
                     /translation="MENDPLPNDSQRRALGEFLQYAFCAMRQEDDPGVTQALADALHN
                     IPVEMYAPGVWSVRRTRRDLMIFEKRHGGSFVEMFDEIFPPNADN"
     gene            complement(1257..1904)
                     /locus_tag="VN496_02585"
     CDS             complement(1257..1904)
                     /locus_tag="VN496_02585"
                     /inference="COORDINATES: protein motif:HMM:NF037890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HXF15859.1"
                     /translation="MRIASPAQLASRLDKNLAWRKQELTQLKFLADSASAQNARVLRR
                     AGIGLLYAHWEGFVKDTSNSYLKYLARGTMEVGKLKSCFVAIALSGDILSAGQAKKTS
                     SHARLVDIVRDLGTPPPATKRIPTKGVISTRSNLKGEVLREIAATLGIDYAPFALKEK
                     PVIDRLVQYRNSIVHGKGLPIQQNEYEELHREIISLMDTYKDLIQDAADGDGHLR"
     gene            complement(1901..>2487)
                     /locus_tag="VN496_02590"
     CDS             complement(1901..>2487)
                     /locus_tag="VN496_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15860.1"
                     /translation="LSAQELRNCILISIDRNLYKWLNALANYENFRRCISLPEKLTKE
                     AYDVELALRFVLLRKLAMSELPASGVALGEYLTKKMIAVAGSKKLDTDKEEKAFKGTF
                     DLLAAALDADSFRKYEIEKERFSGGFSVAAYEVIALGIGSDAEKNKTKTPDSIAKLVK
                     TKVWSNPTFLTSTGLAAASRLPTTLARGRSIFAK"
BASE COUNT          549 a          697 c          663 g          578 t
ORIGIN      
        1 gcggttcatg atggacctgc gcacgcggct ggtgaccggg aatatcttcc agggtgacga
       61 tgtggttcag ctcagcaccg atggctttac gtgctatccg gaagctgtcg atctggcctt
      121 tggcccgcac gtcaagttcg gaaccatcgt gaaggaattt cgcaacgcgg tgcggcccta
      181 cacgccatcg gaaatagtgg gcagccggcg acggggcata ttcaacataa gcggccggga
      241 gcaaacccgc acgatatgca ccagccacgt tgagcggtcg aacctgacga tccgcacgtt
      301 catgcgacgg ttcacccggt tgacgctggg cttctccaag aagctagaga accttgccgc
      361 agctttcgct ctccacgctg cccattacaa cttctgccgg gttcacggca cgcttggaaa
      421 gaccccggca atggctgctg gcgtgaccaa cgatatttgg actttggcgg acctgtacga
      481 aaaggccctc ggatgaatgc ggtgataccg tttgaaatcg gtcaaggaaa accggctagc
      541 ggcgctttcg gcgctcaaaa gcctcgaagg ccactccgca aagcgatcca agcaccgcgc
      601 caagaatcaa gaacaagagc atgtctgagg tgtttgaggt gcggccttct gcgactgcca
      661 gaagcgaccc aagcgccatg ccgaaggcag cgccaattga gagtcgtagc ttgttctcca
      721 tgcccaccat cataccccac gacgcaaatt caatgaaaga aaattcaaag tgtcccacta
      781 cccacagccg ggtagtgata tcgtttgaat cacgaatttt cgcgtttggt acgcgatttg
      841 atttaacgac tcactccgcc actgcggcca gcgctcggga cggggatggt aaagggtcat
      901 gattagttgt cggcgttggg cggaaagatc tcgtcaaaca tctcaacgaa actgccgcca
      961 tgacgcttct cgaatatcat taagtcgcgc ctcgttctcc gcacgctcca aactcctggc
     1021 gcgtacattt cgacgggaat attatggagc gcgtcggcca gcgcctgcgt aacgcctgga
     1081 tcgtcttctt gccgcatggc gcaaaatgcg tattgaagaa actcgcccag agcccgacgc
     1141 tggctgtcat ttggtaatgg atcgttctcc atgtcctcat gatacccgaa cggagagcgg
     1201 gggcggaggg ttgctgacca acgcgttgcc ctaggccgga gcccaacgcg ttggccctat
     1261 cgcaggtgtc catcaccatc ggcagcatct tgaatcaggt ctttgtaggt atccataagg
     1321 ctgataatct cacgatgcag ttcctcgtac tcattctgct ggatgggaag ccccttgcca
     1381 tgaacgattg aattgcggta ctgaaccaag cgatcaatta ccggcttctc cttcaatgca
     1441 aacggggcgt agtcaatgcc cagggtcgcg gcaatttccc tgagcacctc accctttagg
     1501 tttgatctgg tcgaaattac gcccttagtt ggaatccgct tagtcgctgg cggtggcgtg
     1561 cccaggtccc tcacgatgtc aactaaacga gcatgcgacg atgtcttctt tgcctgacca
     1621 gccgacagaa tgtccccgct aagtgcaatg gcgacaaaac atgatttcaa ttttccgact
     1681 tccattgtgc cgcgcgcaag atacttcaga taggaattcg atgtgtcctt aacgaacccc
     1741 tcccagtgag cgtacagaag gccgatgcca gctcgtcgca acacgcgtgc attttgcgcg
     1801 gatgcgctgt cggccaagaa cttgagctga gtcagctcct gttttcgcca tgccagattt
     1861 ttgtcgagtc gacttgccag ctgggctggg ctagcgattc tcatttggcg aagatgctcc
     1921 gcccgcgtgc tagggtggtt ggcagtcgag aggccgccgc aagtccagtg gaggtcagga
     1981 aagtcggatt tgaccacacc ttagtcttga ccagcttggc gatgctgtcg ggggtcttgg
     2041 tcttgttttt ctcagcatca gacccgatcc ccagcgcgat gacttcatat gcggcgacag
     2101 agaatccgcc gctgaaccgt tctttttcga tttcgtattt gcggaaactg tcggcatcca
     2161 aagctgccgc aagcaaatcg aatgtcccct tgaaggcttt ttcctcttta tctgtgtcaa
     2221 gttttttaga accggcaacg gcgatcatct tcttcgtaag gtactcgcca agcgccacgc
     2281 cggacgcggg caactcagac atcgccagct ttctcaacaa cacgaatcgc aatgcgagtt
     2341 caacgtcata ggcttccttt gtcaatttct ctggcagtga gatgcaccta cggaagtttt
     2401 cgtaatttgc caacgcgttc agccatttat agagattcct atctatgcta atcagtatgc
     2461 agttgcgcag ttcctgtgcg gacagga
//
LOCUS       DATMZQ010000082         3574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4176660, whole genome shotgun sequence.
ACCESSION   DATMZQ010000082 DATMZQ010000000
VERSION     DATMZQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4176660"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..556
                     /locus_tag="VN496_02595"
     CDS             236..556
                     /locus_tag="VN496_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HXF15861.1"
                     /translation="MGLCLLMRVIALSTLRDFWSGKSAYSDAREPALAWYRHVLRADW
                     SAPNDVKRDFGTASILRDGRVVFNIGGNKYRLIVWINYPYRVVYIRFIGTHLQYDKID
                     AQRI"
     gene            568..942
                     /locus_tag="VN496_02600"
     CDS             568..942
                     /locus_tag="VN496_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXF15862.1"
                     /translation="MQIKPIRTKADHRAALKEVEGLMSAGKGTRDGERLDVLVTLVEA
                     YERKHFLMELPDPIEAIRFCMEQKGLTAKDLVPMIGQINRVYEVLNGKRPLTLQMIRR
                     LHRDLGIPAESLIGEIGERRAA"
     gene            1151..2323
                     /locus_tag="VN496_02605"
     CDS             1151..2323
                     /locus_tag="VN496_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXF15863.1"
                     /translation="MPRFDPSRPHNTLPQLPPKADIETRSILKACIDARAAVAALQQA
                     GTLIPNQGVLINTIPLLEAQASSEIENIVTTTDALFRYAQIDPERADAATKEALRYRT
                     ALRLGVESLKAKPLSTSTAVAVCSTIQGRDMEIRRVPGTTLTNPARKTVIYTPPVGEA
                     LLREKLANWERFIHDQTGIDPLIRMAVAHYQFEAIHPFADGNGRTGRVLNQLMLVEQG
                     LLDLPVLYLSRYIIGNKADYYRLLLAVTRDATWEKWILYMLAGVRETAVWTVEKIQAI
                     HKLMGHTAEYVRSHATKIYQRELVELIFVQPYCRIQNLVEAELGNRQTAAVYLKTLAN
                     IGVLKEVKAGREKLFVHPKFIALLTSETHKFKSYGAVEQKPVATKGATRRVRGNRS"
     gene            2474..2758
                     /locus_tag="VN496_02610"
     CDS             2474..2758
                     /locus_tag="VN496_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXF15864.1"
                     /translation="MATKITKSSGNVFLDLGFPPHEAAVMLLRAQLAEALRAWMEQEG
                     FTQADAARKLGIAQPRVSEITRGKVELLSLDYLVGLCAKAGIPVKMRLAA"
BASE COUNT          811 a          961 c         1048 g          754 t
ORIGIN      
        1 tatcgcgtca tccgccaggc gcagctgcag cgcttcgacg gtaagcagtg ggtcaggttt
       61 ggggaaatga ttgcgaattg agaagggtcg ctgggccgat cgcaggccgg tgactaggta
      121 gctttcttga aagtaaatac ccgtctgtcc tctgtcgtcc gtcttctgtt cgctgatgtg
      181 gggcggccct tagcttcggt ctcttcgctc ttgtatccct tgcgatagtc ccaaaatggg
      241 actatgcttg ctcatgcgcg ttattgctct ttccactctg cgcgattttt ggagcggcaa
      301 gtcggcgtac tctgatgcac gagagccggc gctcgcgtgg tatcgacatg tgctcagagc
      361 ggactggagt gcaccgaacg acgtgaaacg ggacttcggc actgccagca tcctgcgtga
      421 tggcagggta gtttttaata ttggcggcaa caagtaccgg cttattgtgt ggataaacta
      481 tccatatcgc gtggtgtaca tacgattcat cggaacgcat ttgcaatacg acaagatcga
      541 tgcgcaaagg atatagagga agagatcatg caaataaagc ctatcaggac gaaagccgat
      601 catcgtgccg cgctgaaaga agtcgaggga ctgatgtcgg ccgggaaagg gacgcgcgat
      661 ggtgaacggc tggatgtgct ggtgacgctg gttgaggcat acgaacgaaa acacttcctg
      721 atggaactgc ccgatcctat tgaagccatc cgcttctgca tggagcagaa aggcctgaca
      781 gcaaaagacc ttgtgccgat gatcggccag atcaaccgtg tttacgaagt actcaacggc
      841 aagcgaccgt tgacgcttca gatgatccgg cggctgcacc gcgacctggg aataccggcc
      901 gaatcgctga tcggagaaat aggggagagg cgggcagcct gaggggggag cccctttcgg
      961 tacatcgctt cccccgtcac ccgttatggt ggtggctcag tacccgctct tagttgagcg
     1021 ctaaagaccc aatctgtcct ctgctggcga tatgtcgaaa aactttactt ttttcgacat
     1081 attgtggtga tatgtcgaat cgttcgacat gtcccgccga tgtgtcgaaa aacacggagc
     1141 atttcgacac atgcccaggt tcgaccccag ccgtccccat aacacgctgc cccaactgcc
     1201 gccgaaggca gacatcgaaa cccgttcgat cctcaaggcc tgcatcgacg cccgtgctgc
     1261 agtggcggcg ctgcagcagg cgggtacgct gattcccaac cagggcgtgt tgatcaatac
     1321 gatcccattg ttggaagcac aggccagttc cgaaatcgag aacattgtta cgaccaccga
     1381 cgcgctgttc aggtatgcgc aaatcgaccc ggagagagca gacgccgcta cgaaggaagc
     1441 gttgcgttac cgcacggcgc tgcgtctggg cgtggaatcg ttgaaagcga agccgctcag
     1501 cacgtccact gccgttgcgg tttgctccac catccagggt cgcgacatgg aaatccggcg
     1561 cgtgccggga acaacgctca caaacccggc caggaagacc gttatctaca cgcccccggt
     1621 cggcgaggcc ttgttacggg aaaaactcgc caactgggaa cgattcatcc atgaccagac
     1681 ggggatcgat cccctgatcc gcatggcggt tgcgcattac cagttcgagg caatccaccc
     1741 atttgccgac ggaaatggcc ggaccggtcg cgtgctcaac cagcttatgc tggtcgagca
     1801 aggactgctg gacttgccgg ttctgtatct ttcgcgttac atcattggta acaaggcgga
     1861 ctactatcgc cttctgctgg ccgtgacacg ggatgccacc tgggaaaaat ggatcctcta
     1921 catgcttgcg ggtgttcggg aaacggcagt atggacggtc gagaaaatac aagcgatcca
     1981 caaactcatg ggccataccg cggaatacgt tcgttcccat gcgacgaaga tttatcaacg
     2041 cgagctggtc gaactgattt tcgtgcaacc ttactgccgc attcagaatc tcgtggaggc
     2101 ggagttgggg aataggcaaa ccgcagccgt ttacctgaag acgctcgcca atatcggagt
     2161 tctcaaggaa gtcaaagcag gccgtgagaa attattcgtc catcccaagt ttatagcgct
     2221 gctgacgagc gaaacgcata aattcaaatc ctacggggcc gtggagcaaa agcccgtcgc
     2281 aacaaaaggc gccacgcgac gcgtacgcgg gaatagaagc tgagccccac cggacatgta
     2341 atgacgtcaa atatcggttc cagggttatc gtcgcatgac tggttatgcg catgccacat
     2401 aaccgatatc cgagcggaaa gccgcaaccg gaacccagcg cgacgtaaag gcgatagtcg
     2461 aaaaggagta atgatggcta ctaaaatcac aaagtccagc ggcaacgtgt ttctcgatct
     2521 gggctttccg ccccacgagg ctgcggtaat gttgttgcgt gcgcagctcg ccgaagcgct
     2581 gcgcgcgtgg atggagcaag aaggtttcac gcaggcggat gccgcgagaa agctgggtat
     2641 cgctcagcca cgcgtatcgg aaatcacccg cggtaaggtg gagttactgt cgctcgatta
     2701 cctcgtgggt ctctgcgcca aggctggcat tccggtgaag atgcggctcg ccgcctgaaa
     2761 ctgccagcgc gactattgac tgcgccgtgg agctttattc gattcgtcac gccgttgaag
     2821 gaaatgaagg ttgcaagagt tcgacgtagc ttttttgagc aagagccgct tgaggttggc
     2881 caagcgctca ttggcatgga gctcgggtca gatttaacca acagcgcgtg cggcggactg
     2941 attgtcgaaa cagaggtgta tgttggtcca gaggatcgcg cgtctcattc ctggaacgcg
     3001 cgtagaaccg ctcgtactga agttatgttt ggtccgcgtg gctactcata cgtctttcag
     3061 atttacggca tgcactactg cttcaacata gtcgttggtc cagggcgaat gcctgcggcc
     3121 gtgttgatta gagcgctgcg gccaacgttc gggttggaaa tcatggctaa caaccgcggt
     3181 ctttcaagcg tgccggcaaa cctgcggctg cttacctctg gcccaggacg attggcgcaa
     3241 gcactcggaa tcgctcgcca ggcaaacggg ttggatctat gtcaggcgcc gctgtgggtt
     3301 cggcgctctg cggttggacg ccgcctcgcc gctgcccatg gaatccgagc cggggctcgt
     3361 atcaacgtag agtatgccgg gcagtgggca aggcgaaact ggcgatttac cctctcgggg
     3421 gatcgattcg tcagcgttgc tacctgactc gatgtgggga ccggcgaggc acataaaaag
     3481 cgcctcactt cgacgcaatc cgcttctgca tgaaacagaa aagcctgaca gccaaagatc
     3541 tcattccgat gagcggcgga tcaaccgcgt ttac
//
LOCUS       DATMZQ010000083         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2483597, whole genome shotgun sequence.
ACCESSION   DATMZQ010000083 DATMZQ010000000
VERSION     DATMZQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483597"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1610)
                     /locus_tag="VN496_02615"
     CDS             complement(183..1610)
                     /locus_tag="VN496_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF15865.1"
                     /translation="MPSDDFTAFDAIALADLVRRKELAPIDLVDAAIARIESLNPRIN
                     AVVTRMYERARTIAGGRLSAGPFTGVPFLLKDSLATYAGVPTSSASRLLRGFPADHDS
                     ELVVRLKRAGLIVLGKTNCAEFGLLPTSEPSEFGATCNPWDPLRTPGGSSGGSAAAVA
                     AGMVTMAHGADGGGSIRIPASCCGVFGLKPTRARNPAGPDHGDVRSGLVVEHALTNSV
                     RDSAALLDATAGPDVGDPYCAPVPTHPFLHEVGVDPGRLRIAYSAHTFAPLHHDCALA
                     LQDAAMLCQELGHDVIEARPRIDYLRTAQLFTTLWTAGCAATIDGIARESNKCIDDSD
                     VEPVTWKLYEMGRRVSAAEYQLAISELQLISRSIARFFLEYDIWLTPVVTEPPPVLGS
                     FEAPPEDLFAAFRRAWEFAPITGLCNVTGQPAMSVPLHWNADGLPVGSHFAGRFGDEA
                     TLFRLAGQLEQSRPWKARRPALSAQ"
     gene            complement(1620..>2343)
                     /locus_tag="VN496_02620"
     CDS             complement(1620..>2343)
                     /locus_tag="VN496_02620"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF15866.1"
                     /translation="RASFVLVALWWAVFAVPLWLFVNEEPRTMTGAAAAKEGLRQVAA
                     TFRQLRGLHPVWLFLLAYWFYIDGVYTIARMALDYGLALGFDPKSLIVALLITQFVGF
                     PAALAFGYAGERLGAKPAIFVAIAAYAGATLWSYAMTEVWEFYALATAIGLVQGGAQS
                     LSRSFYARIIPRAQAGEFFGFYNMAGKFGSILGPMLVGVAGALTGSPRLGILAVLLLL
                     IVGTLLLSLVPADHARSLYPHG"
BASE COUNT          497 a          733 c          708 g          405 t
ORIGIN      
        1 cgaccggctg cgagcgatga cggcgcagaa aaagtaaggg tcagggctaa aagtagatcg
       61 cgcagtccgg taatcctgcc cgcatcttgt cgctttatcg ccgccccttt ttggtccacg
      121 ggccggtcta gctgagcggg ccactgcgtc gcagcgctca tttttggatc ggcggccgaa
      181 tgtcactgcg ccgacaacgc cggtctgcgc gccttccacg ggcgactctg ttccagctgt
      241 ccggcaaggc ggaacaatgt ggcttcatcg ccgaatcggc cagcgaaatg cgatccaacc
      301 ggcagcccgt ccgcgttcca atggagcggc accgacatgg cgggttgacc ggttacgttg
      361 caaagaccgg tgatcggcgc gaattcccat gcgcgacgga acgccgcaaa cagatcctct
      421 ggcggcgcct cgaagctgcc gagaactggc ggcggttccg tcaccaccgg cgtgagccat
      481 atgtcgtatt cgaggaaaaa tcgcgcaatg ctccgggata tcagttgcaa ttccgatatg
      541 gcaagttggt attcggcagc gcttacgcgg cgccccatct cgtacagctt ccaggtaacc
      601 ggttcgacat cactgtcgtc gatacatttg ttgctctcgc gcgcgatacc gtcgatggtt
      661 gcagcgcaac cggccgtcca caaggtggtg aacaattgcg cggtcctcaa gtagtcgatg
      721 cgaggccgtg cttcaattac gtcgtggccg agttcctggc aaagcatcgc ggcatcttgc
      781 aatgcaagcg cacaatcgtg gtgaagaggt gcaaaggtgt gtgcactgta cgctatgcgc
      841 agccgccccg gatcgacgcc gacctcatgc agaaatggat gtgtgggcac gggcgcgcag
      901 tatgggtcgc ccacatcggg ccccgcagtc gcatcgagaa gcgccgcact atcgcgcacc
      961 gaattcgtca gggcatgctc gaccacgagt ccgctgcgca cgtcgccatg gtcgggtccc
     1021 gctggattgc gcgcccgtgt gggcttcaat ccgaacacac cgcaacacga agccgggatg
     1081 cggatcgagc ctcctccgtc ggcgccgtgt gccatcgtca ccatgcccgc cgctaccgcg
     1141 gctgccgagc caccactgga tccgcccggt gtgcgcaagg ggtcccaagg attgcaggtt
     1201 gcaccgaact cgctcggctc gctggttggc agaagtccga attctgcaca gttggttttg
     1261 ccaagcacga tcaaccctgc ccgcttgagc cttacgacga gttcgctatc gtgatcggcc
     1321 gggaagccgc gcagcagtcg cgacgcggaa gacgttggta cgcccgcata agttgcgagg
     1381 ctgtccttca gcaggaaggg aacgccagtg aacgggcctg cggacaaacg ccctccagcg
     1441 atcgttcggg cccgctcgta catccttgtc accaccgcgt tgatacgcgg attcagcgat
     1501 tcgatgcgcg caatcgcggc atccaccaag tcgatcggtg caagctcctt gcgccgaacc
     1561 agatcggcca acgcgatggc atcgaacgct gtgaaatcat ctgacggcat cgctgcgact
     1621 taaccgtgcg ggtacaacga gcgcgcatgg tcggccggta ccagggataa caacaaggtg
     1681 ccgacgatga gcaggagcaa aaccgccaga atcccgaggc gcggactgcc ggtgagtgcg
     1741 ccggcaactc cgaccagcat gggccccagg atcgagccaa attttccggc catgttgtag
     1801 aagccgaaga actctccggc ttgggctcgc ggaatgatgc gggcatagaa ggatcgcgac
     1861 agggactgcg cgccgccttg cacgaggcca atcgccgtcg ccagcgcata gaattcccaa
     1921 acctcggtca ttgcgtaact ccatagcgtt gcgcccgcgt aggcggcaat ggccacgaag
     1981 attgcaggct tggcgccgag acgttccccc gcataaccga aggcaagagc agccgggaag
     2041 ccgacaaact gcgttatcag aagcgccact atcaaacttt tcggatcgaa gcccagcgcc
     2101 aggccatagt cgagagccat gcgtgcaatc gtgtaaacac cgtcgatgta aaaccagtag
     2161 gcaagcaaaa acagccagac ggggtgcaag ccccgcaatt gccggaaagt ggccgcaacc
     2221 tggcggagcc cctcctttgc agcggccgcg ccagtcatgg tccgtggttc ctcattgacg
     2281 aacagccata agggaacggc gaacacagcc caccacagcg cgaccagtac aaatgacgca
     2341 cgt
//
LOCUS       DATMZQ010000084         2764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1794394, whole genome shotgun sequence.
ACCESSION   DATMZQ010000084 DATMZQ010000000
VERSION     DATMZQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794394"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..920)
                     /locus_tag="VN496_02625"
     CDS             complement(33..920)
                     /locus_tag="VN496_02625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF15867.1"
                     /translation="MSKWAKNNHVECYRIYDRDIPELAFALDIYAERAHLQQYSEPAM
                     APETHRDWLTAMHQAAARALGRPLKDITLKQRQGQRPEQQYRKLATQAADFVVQEGGH
                     RFIVNLTGHLDTGLFLDHRQTRALVGSLARGKRVLNLFCYTGSFSVYAAKNQATATTS
                     VDLSKTYLDWARRNFDLNNIDRAKNRLVQADVLRFIETERASRDRYELIVLDPPSFSN
                     SKRMKGVLDVQRDHAALIRGCLELLARGGELLFSTNLRTFRLDTSALADLPMQEISAQ
                     TVPPDFRNRRIHRCWRVKP"
     gene            1080..1739
                     /locus_tag="VN496_02630"
     CDS             1080..1739
                     /locus_tag="VN496_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXF15868.1"
                     /translation="MMTGLDSSLKTLAVWVLVVASSSVFAQGSIAPSAIADPWYGGQN
                     KGFWGLSDSVGVRGALPGTSTFTPPGTSADSLNAVRQFGGYRIGHGFAIEGSQTQFGD
                     ASSGCEGGAANAGRACYGSTWSLSGVATLPFQSGLSLYGRLGLHYWQKGAQDEINHRA
                     DEPLGKVYGIGLSYEMTKSITVHAESERYSELTGSNAYGPSGGVGLDSSVHSIGLSVR
                     F"
     gene            2151..>2764
                     /locus_tag="VN496_02635"
     CDS             2151..>2764
                     /locus_tag="VN496_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HXF15869.1"
                     /translation="MRIYMIGSGAMGGVYGGLLAGAGYDVTLIDSREDHIHKIQTEGL
                     TVEGMRGTHVVRVAAQRDETGLAPGDLAIIFTDANATGEAAKLATKLLKPDGFALTLQ
                     NGIGNVETLVAALGKQRVIAGVSMNSAANPATGRSVYTNVGMTSIGELDGASTQRVKQ
                     VADMLNNAGIPTEIIPDPMNWIWGKFVLNCGINALTAITGLRSG"
BASE COUNT          586 a          748 c          823 g          607 t
ORIGIN      
        1 agtctttccg acattcacga tattcacggc agttaaggct tcactcgcca gcaccggtga
       61 attctgcgat tgcggaaatc cggcggcacg gtctgcgcgg atatttcctg catgggcaaa
      121 tcggctaacg cggaagtatc cagacggaag gtgcgcaggt tggttgagaa cagcagttca
      181 cctccgcgcg ccagtagttc caggcagccg cgaatcagcg cggcatgatc gcgctggaca
      241 tccagcacgc ctttcatgcg tttggaattc gaaaagctcg gtgggtcgag gacgatgagt
      301 tcgtagcgat cgcgggaagc gcgttcggtc tcgataaaac gcagtacgtc tgcctgcacc
      361 aggcggttct ttgcccggtc aatgttgttg agatcgaaat tgcgcctggc ccaatcgaga
      421 taggttttcg acagatccac gctcgtcgtt gcagtggcct ggtttttggc cgcgtaaacg
      481 ctgaagcttc ccgtgtagca aaacagattc agcacacgct tgccgcgggc cagggatccg
      541 accagtgcgc gggtctggcg atgatcaaga aacagcccgg tatccaggtg cccggtcagg
      601 ttgacgatga atcgatgccc accctcctgc acgacaaaat cggctgcctg tgttgccagc
      661 ttgcggtact gctgctccgg gcgctggcct tgccgctgct tcagcgtaat gtctttcagc
      721 ggtcggccca gcgcacgagc ggcagcctga tgcatggccg taagccagtc gcgatgcgtt
      781 tcgggcgcca tcgccggttc actgtattgt tgcagatgcg cacgttcagc atagatatcg
      841 agcgcgaagg caagctcggg aatatcgcga tcatagatcc tgtaacactc gacgtggttg
      901 tttttcgccc atttagacaa gtgtttatga tttttcgcga tcctgttggc gaacatttct
      961 gcctggcggt gagtctgagc accaggtcct tccggagtgt tgtattgctg cgattccatg
     1021 gaaatggggc tgacaaggct aacaatttcg ctaaaattgc tgcctaggag tttcagacaa
     1081 tgatgactgg ccttgactca tcgctcaaga ctcttgccgt gtgggtgtta gttgtggcga
     1141 gcagttcggt gtttgctcag ggcagcattg ctccctcggc aattgcggat ccctggtacg
     1201 gcggccagaa caaaggcttc tggggattga gcgattcagt tggcgtgcgg ggcgctctgc
     1261 ctggcacatc gacctttacc cctcccggca ccagtgcgga tagtctcaac gcggtacggc
     1321 aattcggggg atatcggata ggccatggtt ttgccatcga aggctcccag acgcagttcg
     1381 gcgatgccag ttcgggctgc gaaggcggtg ccgcgaacgc cggtcgtgcc tgctacggtt
     1441 ctacctggag tttgagcggc gtcgccaccc tgcccttcca atccggtttg tctctctatg
     1501 gacgcttggg cctgcactac tggcaaaaag gcgcgcagga cgagatcaac catcgtgcgg
     1561 atgaacccct cggcaaggtt tacggcatcg gtttgagcta tgaaatgacg aagtcgatca
     1621 ctgtccacgc cgaatcggag cgttactcgg agttgaccgg tagcaatgcg tacggcccga
     1681 gcggcggcgt gggactggac tcttccgtgc attcgatagg actgtctgtc agattctgag
     1741 cgaacgatcg ggaaaaagga ctgaggactg tcccgcaagg ggataccggt ctaaaagagt
     1801 actccgagct tcgctagggt attcgaccat aaggtatgag gactgaggaa aaaacatacc
     1861 accacagcta cagccgtgag tgtgccggtt agatacgaat accctcgcgt agcgtggagt
     1921 acccttttag acacggagaa aaaccaagac tggaaaaatg aagggccgcg acgcggccct
     1981 tttgttttga tgacgtcaag ggaatgcgca caattcttcc gccctgcttt tactccgtcc
     2041 tcgtacctca gtcctgcact tttcttccac tcagtcctca gttatgggca gcgcccggta
     2101 tcctgcgaag cagggcctcg tacctcagtc ctaattactt cggaggagcc atgcgtattt
     2161 acatgatcgg atcgggagcg atgggtggcg tctacggcgg cctgcttgct ggcgccggct
     2221 acgatgtcac gctgatcgat tcgcgggaag accacatcca taaaatccag acggaaggct
     2281 tgaccgtcga aggaatgcgt ggaacccatg tcgtcagagt tgcggcgcaa cgggacgaaa
     2341 ccggccttgc accgggcgat ttagcgatca tattcaccga tgccaatgcg actggcgaag
     2401 cagcaaagct cgcaacgaaa ttgctcaagc cggatggttt cgcgcttacg ctgcagaatg
     2461 gcattggcaa tgtcgaaacg ctcgttgcgg cactcggcaa acagcgtgtc attgcgggag
     2521 tgtcgatgaa cagcgcagcc aacccggcga ccggccgctc ggtgtatacg aatgtcggca
     2581 tgacttccat cggggaactc gatggcgcca gtacacaacg cgtgaagcag gtcgcagata
     2641 tgctgaataa cgcgggcatt ccgaccgaaa tcatcccgga cccgatgaac tggatctggg
     2701 ggaaatttgt gctgaactgc ggcatcaatg cgcttaccgc aatcaccggc ctgcgcagtg
     2761 gcga
//
LOCUS       DATMZQ010000085         8757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1795352, whole genome shotgun sequence.
ACCESSION   DATMZQ010000085 DATMZQ010000000
VERSION     DATMZQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795352"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VN496_02640"
     CDS             complement(<1..608)
                     /locus_tag="VN496_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HXF15870.1"
                     /translation="MNTEMRRHFMAPLVRGFIICLLGCPIVVSAGAVSEIDTPPSTND
                     SFGSAQHLSVPPDGLSVSAVIGQLSGDWNTDVDFFTFEGSEGDTPGISTIGACPAFPS
                     NLVLYDAWGNIRGSAAGNCDDGTEALINAVTLPATGTYVVGVSAWTHTFLENGVVENA
                     DAQTPGGAYQLVIAGVHNPTPVPPPQDPPPPPPPQDPPPPPPP"
     gene            complement(928..1854)
                     /gene="cysB"
                     /locus_tag="VN496_02645"
     CDS             complement(928..1854)
                     /gene="cysB"
                     /locus_tag="VN496_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HXF15871.1"
                     /translation="MKLQQLRYICEVARQSLNLSTAAEVLHTSQPGISKQIRSLEEEI
                     GVDIFVRHGKRVVAVTEPGKAILDIAQRILKDAENLKQVGEQFTEQDKGRLTIATTHT
                     QARYALPQVIQGFTKRYPEVRLALRQGSPTQISEMVTSGEADIAIATEAIELYEDLVM
                     LPCYKWNRCLLVQPGHPLLKIKKLTVEAIAAFPIITYDFAFTGRSKINQAFADKGLTP
                     NVVLTAIDADVIKTYVELGLGVGIVAMMAYDPKRDTHVRAIDASHLFEPSTTRIGIRK
                     NSYLRGYTYDFIEMFAPQLTRKVVNDAMRSKG"
     gene            1971..2744
                     /locus_tag="VN496_02650"
     CDS             1971..2744
                     /locus_tag="VN496_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXF15872.1"
                     /translation="MELGYALSGLLVGFIVGLTGVGGGSLMTPLLVMGFGISPATAVG
                     TDLLYASFTKATGVWVHARRGNVEWRLVGLLAAGSIPAALAALLCLHYLGVDSRRVGG
                     LITGSLGIALILTALAILFKERLQRLSRHGAGQKWRERHVVAATITTGAVIGALVALS
                     SVGAGALGVAALFWLFPLFPASRIVGSDIAHAVPLTAVAGLGHFYMGTVDWHLLVNLL
                     IGSLPGIYVGSHLSGRIADRWLRPVLASMLVLVGGKLVL"
     gene            2790..4496
                     /locus_tag="VN496_02655"
     CDS             2790..4496
                     /locus_tag="VN496_02655"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HXF15873.1"
                     /translation="MYVYDEVDRNLVNQRVAQYRDQVSRFLNGDLSEDEFRPLRLQNG
                     LYIQRHAPMLRVAIPYGLLSSTQLRMLASIARRYDRGYGHFTTRQNIQYNWPKLEDTP
                     DILADLASVQMHAIQTSGNCVRNITSDEFAGIAPDEILDPRPFAEILRQWSTFHPEFA
                     FLPRKFKIAVNGAREDRAATAFHDIGLHLQRDAAGEVGFRVIVGGGMGRTPIVGPVIR
                     EFLPWRHMNTYVEAVLRVYNRHGRRDNLYKSRIKILVKALGAEEFAHQVEQEWAQTRE
                     GPDTLNEQELARVSAYFTAPDYGAQPADDRDFDSQVQSSPAFARWVGRAVKPHRVSGY
                     RAIVLSLKKTGVPPGDATADQMDLIAAWSERFGFGEVRVSHEQNLILPDVRANDVYML
                     WEEARTAGLATPNLGLLTDIIACPGGDFCSLANAKSIPIAQAIQHRFDDLDYLHDIGD
                     LNINISGCMNSCGHHHAGHIGILGVDKDGEEWYQVSVGGADGSDAGRGRSAIGKVIGP
                     SFKAADMPDVITRLVDIYLDQRDEEERFIDTVARLGIAPFKSGVYGAPLSQSREGVTA
                     DA"
     gene            4489..5010
                     /locus_tag="VN496_02660"
     CDS             4489..5010
                     /locus_tag="VN496_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HXF15874.1"
                     /translation="MPDIILRDSIVSDDWQVLRPAAGEMAIVPDGRVIVPVAVWLEQQ
                     TELVARGNVGVWLAGADDPAQLTPSLSLLSLVAVDFPKFTDGRGYSIAYLLRSRFGYR
                     GQLRAIGDVLPDQLFYMKRVGFDAFAVRADKDIGQALRSLKPFTDAYQGSWDNPVPAF
                     RRHQRAWPAASKA"
     gene            5026..5763
                     /locus_tag="VN496_02665"
     CDS             5026..5763
                     /locus_tag="VN496_02665"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HXF15875.1"
                     /translation="MHKGFSALNASVQYPHAALLARLEEIAHEFRNVAFASSLGAEDM
                     VLADAILTNRLSITIFTLDTGRLPLETLQLLERVRNSYSQPIEVYTPSKDEVDQYVAT
                     HGHNAFYESVELRKRCCHIRKVNPLARALAGRDAWVTGLRRAQSSTRMELSLREFDAA
                     HGLVKFNPLAEWTEDEVWAYIRDRGVPYNALHDRGYPSVGCDPCTRAIRPGEDIRAGR
                     WWWETRDNKECGIHVSQLVQPDGVKAS"
     gene            5760..6683
                     /gene="cysD"
                     /locus_tag="VN496_02670"
     CDS             5760..6683
                     /gene="cysD"
                     /locus_tag="VN496_02670"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HXF15876.1"
                     /translation="MNAALQIDSIRINHLDWLESESIHIIREVAAECSNPALLFSGGK
                     DSIVMLRLAEKAFRPGKFPFPLLHIDTGHNFPEVMQFRDARVGELGERLVIGHMDDSI
                     RSGRVRLRTAGESRNVHQSVTLLDTIAQHGFDACIGGARRDEEKSRAKERIFSFRDEF
                     GQWDPKNQRPELWSLYNTRVHKGENIRVFPISNWTELDVWQYIEREGLGVPSIYFAHR
                     RDVVRRPGGLLPVSHLLQPQANEVVENISVRFRTVGDMTCTCPVESTAATPAEIIAET
                     SVTRITERGATRMDDQTSEASMELRKKEGYF"
     gene            6685..7962
                     /locus_tag="VN496_02675"
     CDS             6685..7962
                     /locus_tag="VN496_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXF15877.1"
                     /translation="MSAIENMPPVDTGMLRFTSAGSVDDGKSTLIGRLLYDTKAIFED
                     QLSSIEQSSRRRGQIGIDLSLLTDGLLAEREQGITIDVAYRYFATPSRKFIIADTPGH
                     EQYTRNMVTGASTADLAIVLVDARKGLLTQSRRHAYIASLLRIPHVVLAVNKMDLVNY
                     SQAVFDEISTAFNAFVKDLDFHRVDALPMSALQGDMVVWRGENLPWYQGQTLLEILES
                     AEVARSAVHGDLFFPVQLVSRPGDSGVDDARGYAGRIESGRISVGDAITVLPSGQVSR
                     VRMIRTFDGAVRQAQAPQSVTVVLADQLDISRGEVLTATAAPPAVARQFEATLCWLAT
                     EPFNRSRRYLIKHGTRTVKGLLDSPQYKINVNSMEREPADTFALNEIGRISVKTAQPL
                     AVQPYRFNRAAGSFIVIDESNNNTVAAGIIESA"
     gene            7964..8725
                     /gene="cobA"
                     /locus_tag="VN496_02680"
     CDS             7964..8725
                     /gene="cobA"
                     /locus_tag="VN496_02680"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXF15878.1"
                     /translation="MGKVYFVGAGPGAADLITVRGAQLLRRADIVFHDALVQQDMLAL
                     AEKAKLVAVGKRCSQISTDQRFINRNLVEASAKYNVVARLKGGDPTLFGRLQEEIDAL
                     EASGVEYEIVPGVTAALAGSAEIGVSLTRRGMSRSVVFVTPRVGEGEDESDWAQVVAA
                     ADTAVLYMAAGQAEAISRILRQHGMPASTPAVIVVNVSLAVAQRIHTVLERLEHDVPT
                     QLTGPAVLMIGSVFASDRAIRHAGDEALSLTANFR"
BASE COUNT         1800 a         2382 c         2620 g         1955 t
ORIGIN      
        1 ggcggaggcg gcggcggcgg atcttgcggc gggggcggcg gcggcggatc ttgcggcggg
       61 ggcacaggcg taggattgtg gacgccggct ataacgagct gataggcccc accaggagtt
      121 tgcgcgtcgg cattttccac aacgccgttc tcgagaaaag tatgtgtcca tgcgctgacg
      181 ccgacgacgt aggtaccggt tgcgggcaac gtcaccgcgt tgatcaacgc ttccgttccg
      241 tcgtcgcaat ttcccgctgc ggagccccgt atgttgcccc aggcatcgta caggaccaga
      301 ttggaaggaa atgccgggca tgcaccgata gtggaaatac caggcgtatc gccttcgctt
      361 ccctcgaacg tgaagaaatc cacatcagta ttccaatcgc cactgagctg accgataaca
      421 gctgaaacgc tgagcccatc gggcggcaca gagagatgct gtgccgagcc gaatgaatcg
      481 ttggtcgaag ggggtgtatc gatctcggaa acggcacctg cggacactac aattggacaa
      541 ccaagcaaac aaataataaa accgcgaact aacggcgcca taaaatgccg ccgcatctcg
      601 gtattcatga tgacactctc ctgttggatt actgtttcag ggactgatgt aacgccattg
      661 gaatatgcaa tctccatacc caatgccggt ttccgcaatg ccaaaatgca cggtaatttc
      721 ggcccgttag ctatcaatcc cttatgacgg ttagttcttg caatgtcgcg ttgttgattt
      781 ttttgacacg cctaaatcgg ctttgccgaa gtgaattgcg tcatacgacg tattgcgcgc
      841 tacgtgacgc aaaacggtag tcagggaatt tgcataggca tcaaatgcga acgcaggaca
      901 aagagcacgc agtcaagaaa accaggatca gcccttgctg cgcattgcgt cgttgacgac
      961 cttccgcgtg agttgcggcg caaacatttc gataaagtcg taggtgtaac cacgcaaata
     1021 gctgttcttg cggatgccaa tgcgcgttgt gcttggctcg aagaggtgac tcgcgtcgat
     1081 agcgcgcaca tgagtgtcgc gttttggatc ataggccatc attgcaacaa ttccgacccc
     1141 aaggccaagc tcgacatacg tcttgatgac atcggcgtcg attgcggtga gcactacgtt
     1201 tggtgtcagc cccttatccg caaacgcttg attgattttt gaccgtccgg taaaggcgaa
     1261 atcataagta atgatgggaa acgcagcgat tgcctcgacg gttagttttt ttatcttcag
     1321 caatggatgg ccgggctgca ctagaagaca gcggttccac ttgtagcacg gcaacatcac
     1381 cagatcttca taaagttcga ttgcttcggt agcgatcgcg atgtcggctt cgccggaagt
     1441 gaccatttcg gaaatctggg tggggctgcc ttggcgcaat gcaagccgga cttcgggata
     1501 acgctttgtg aagccctgga tgacttgggg gagggcatag cgggcttgtg tgtgcgtggt
     1561 tgcaatcgtc aggcgcccct tgtcttgctc ggtaaattgt tctccgacct gcttgaggtt
     1621 ctctgcatct ttcaatatcc gctgcgcaat atcgaggatt gccttgcccg gttcggtcac
     1681 tgccacgacg cgcttgccgt ggcgcacgaa aatgtctacg ccgatttcct cctcgaggct
     1741 gcggatctgc ttgctgattc cgggctgtga ggtatgcagc acttccgctg cggtggagag
     1801 gttcagactc tggcgggcta cttcgcagat gtagcgcaac tgctgcagtt tcatgtggcg
     1861 ggtccgaagc ttataagaga atcgtatttg atactaacag aatattcata ggaaatataa
     1921 agcccatcag tatcatccgg gtgtcgcgac caagccttgg gtagaggtaa atggagctgg
     1981 gatacgccct gtcgggattg ctggtggggt tcatagtcgg tcttaccggt gtcggcggcg
     2041 gttcactaat gacgcctctg ctcgtcatgg gctttggaat ttccccggca accgccgtgg
     2101 ggactgactt gctatacgcc tcttttacca aggccactgg tgtatgggtc catgcgcgac
     2161 gaggcaacgt ggaatggagg ctcgtgggct tgcttgccgc tggcagcatt cccgcagcac
     2221 tggcagcact gttgtgcttg cattatctcg gtgtcgacag ccgaagggtt ggtggcctga
     2281 tcaccggatc gcttggcatc gccttgattc tgactgcgtt ggctatcctc tttaaagaaa
     2341 ggttgcagcg cctgagtcgt cacggcgcgg gacaaaaatg gcgcgaacga catgtcgtcg
     2401 ccgcgaccat aacgacggga gcggttatcg gcgcactggt cgctttgagt tcggtcggag
     2461 ctggagcgct cggcgtggcg gcgttgttct ggttgttccc gttgttcccg gcgtcgcgga
     2521 tcgtcggcag tgatattgcg cacgcggttc ctttgacggc tgtagctggc ttgggacatt
     2581 tctacatggg cacggtggat tggcacttgc tggtgaatct gctgatcggc tccttgccag
     2641 gcatttatgt cggcagccat ttaagtggtc gcatcgccga ccgctggctg cgtccggtac
     2701 tggcttccat gctggttctc gtcggtggca agctggtcct ctgacaaaca attgtcccgc
     2761 tctgaaacga cgcaaaggcg cgctcactaa tgtacgtata tgacgaagtc gaccggaatc
     2821 tggtcaatca gcgagtggcc cagtaccggg accaggtatc gcgttttctg aacggagact
     2881 tgagcgagga cgagtttcgt ccattgcgcc tgcaaaacgg cctctacatc cagcgccacg
     2941 cgccgatgct gagagttgcg attccctacg gtttgttatc gtccacccaa cttcgcatgt
     3001 tggcatccat tgcgcggcgc tacgatcgcg gctacggtca tttcacgacg cgccagaata
     3061 ttcagtacaa ctggccgaag ctggaagata cgccggacat ccttgccgat ctggcgagcg
     3121 tgcaaatgca tgcgatccag accagcggca attgtgtgcg caatatcacc tcggacgaat
     3181 tcgcgggtat cgcacccgac gagatcctgg atcctcgccc gtttgctgaa attctgcggc
     3241 aatggtccac cttccatccg gagttcgctt tccttccgcg caagttcaag attgccgtca
     3301 atggtgctcg ggaagaccgc gccgcaacgg ccttccacga tataggactt catttgcagc
     3361 gggacgctgc cggggaagtc ggattccggg tgatcgtcgg cggcggcatg ggacgcactc
     3421 cgattgttgg accggtcata cgcgaatttc tgccctggcg gcatatgaac acgtacgtcg
     3481 aagccgtgct gcgtgtctat aaccgccatg gtcgtcggga taatctttat aaatcgcgta
     3541 tcaagatcct ggtgaaggcg ctaggcgcgg aggaatttgc ccaccaagtg gagcaagagt
     3601 gggcgcaaac tcgggagggc cccgatacgc tgaacgaaca ggaattggcc cgtgtatcgg
     3661 cgtatttcac cgctccagac tatggagcac agcctgctga tgaccgtgat ttcgatagcc
     3721 aggtccaaag ctcgccggcg ttcgcccgct gggttggtcg ggccgtcaag ccgcatcgcg
     3781 tgtcaggcta tcgcgccatt gttctgtctc tcaagaagac tggtgtgccg cctggcgatg
     3841 ccaccgcgga ccagatggac ctgattgcag cgtggtccga gcggtttggg ttcggcgaag
     3901 tccgcgtgtc tcacgaacag aacctcattt tgccggatgt gcgcgccaat gacgtgtata
     3961 tgctatggga ggaggcccgg acagcaggtc ttgccacccc aaacctgggc ttactgacgg
     4021 atatcatcgc gtgtccgggc ggggatttct gctctctggc gaacgccaaa tccattccga
     4081 tcgcgcaagc gattcaacac cggttcgatg atctcgacta tttgcacgat atcggagatt
     4141 tgaacatcaa tatttcaggc tgcatgaatt catgcggcca tcatcacgcc ggacacatcg
     4201 gcatactggg cgtcgacaag gacggagaag agtggtacca ggtatcggtg ggcggcgcgg
     4261 acggttcaga cgcaggtcgc gggcgcagcg ctattggcaa ggtgatcggg ccatcattca
     4321 aggcggccga tatgccggac gtcatcacgc gactggttga catttatctg gatcagcgag
     4381 acgaggaaga acggttcatc gacacggtag cgcgcttggg catcgcgccg ttcaagtcgg
     4441 gtgtttatgg cgctcctctg agtcaatctc gtgaaggagt aaccgccgat gcctgacatc
     4501 atccttcgcg attccattgt ctcggacgat tggcaggtct tgcggcccgc ggccggtgag
     4561 atggcaatcg tgccggacgg acgtgtgatt gttcccgttg cagtgtggct cgaacagcaa
     4621 acggaattgg ttgcgcgcgg caatgtgggt gtctggttgg ccggcgcgga tgatcccgcg
     4681 caacttaccc cttcgctgtc actgctgtcg ctggttgccg tggatttccc gaaatttacc
     4741 gacggtcgtg gttactcgat cgcgtatttg cttcgttcgc gtttcggtta ccgtggacag
     4801 ctacgtgcca ttggcgatgt tctaccggat caattgttct atatgaaacg ggtcggattt
     4861 gacgcgttcg cagtgcgtgc cgacaaggat atcgggcaag cattgcgcag ccttaaaccg
     4921 tttaccgatg cctatcaagg ttcttgggac aacccggttc cggcgttccg tcgtcaccaa
     4981 cgcgcttggc ctgcagcctc caaggcatga tgcatgacgg caaacatgca taaaggattt
     5041 tctgctctga acgcttcggt tcaataccct catgccgcgt tgttggcgcg gctcgaggaa
     5101 atcgcgcacg aatttcgcaa tgtcgcgttc gcgagcagtc tcggggcgga agacatggtg
     5161 ctggccgacg caatattaac gaaccggctt tcgatcacca ttttcacgct tgataccggc
     5221 cgactgccgc tcgaaacact gcaactgctg gagcgcgtgc gtaattccta ttcgcagcca
     5281 atcgaagtct atacaccttc caaagatgaa gtcgaccagt acgtcgcgac gcatggccat
     5341 aacgccttct atgaaagcgt ggaattacgt aagcgttgct gccatattcg caaagtgaac
     5401 ccgttggctc gcgcgctcgc aggacgcgat gcctgggtga ccgggctacg gcgcgcccag
     5461 tccagcaccc gcatggaatt gtcgcttcgg gagtttgacg ctgcgcatgg tttggtcaaa
     5521 ttcaatccat tggcggaatg gaccgaggat gaggtttggg cgtatatccg cgaccgcggc
     5581 gtgccataca acgcactcca tgatcgcggc tatccatccg tgggctgcga tccgtgcacg
     5641 cgcgcgatac gtccaggtga ggatattcgc gcgggacgtt ggtggtggga aaccagggac
     5701 aacaaagaat gcggcattca cgtttcccag ttggtacaac cggatggagt aaaggcatcg
     5761 tgaacgcggc actgcaaatc gattctatac gcatcaatca tctcgattgg cttgaatccg
     5821 agtcaatcca tatcattcgc gaagtagcgg cggagtgcag caatccagcg ttgctttttt
     5881 ccggaggcaa ggattccatc gtgatgctgc gcctcgcgga gaaggcgttc cgcccgggga
     5941 aatttccatt ccctcttctg catatcgata ccgggcacaa ttttccggaa gtcatgcaat
     6001 tccgcgatgc ccgtgtcggt gaactgggcg agcgcctggt gattggccac atggacgact
     6061 caatccggtc cggccgggtg agattgcgca ccgcagggga aagccgcaac gtgcatcagt
     6121 cggtcacgct gctggacacc atagcgcaac acggttttga tgcctgtatt ggaggcgcgc
     6181 ggcgcgatga ggaaaaatcc cgtgccaagg aacgtatttt ttcttttcgc gacgagtttg
     6241 gccaatggga tcccaagaac cagcgaccgg aactttggag cctttacaac acacgcgtgc
     6301 acaaggggga gaacatacgg gttttcccga tcagcaactg gaccgagctg gatgtgtggc
     6361 aatacatcga gcgtgaaggc ctgggcgtgc cgtccattta tttcgcacac cgtcgtgatg
     6421 tcgtgcgccg gcctggcgga ttgttgccgg tatcgcatct gttacagccc caagcaaatg
     6481 aagtggtcga aaacatctca gttcgatttc gcacggtcgg cgatatgacc tgcacttgcc
     6541 ctgtggaatc tactgctgca acgcccgcag aaatcatcgc ggagacgtcg gtgacccgga
     6601 tcaccgagcg cggcgctacc cgcatggacg atcagacttc ggaggcttcc atggaattgc
     6661 gcaagaagga aggatatttc tagcgtgtca gcgatcgaga atatgcctcc tgtggatacc
     6721 ggcatgctgc gctttaccag cgccggcagt gtcgacgatg gcaaaagtac gttgattggc
     6781 cggttgctgt acgacaccaa ggccatattc gaagatcagc tttcttccat cgagcaatcc
     6841 agccgccggc gcggacagat cggtatcgat ttgtcattgc tgaccgatgg actgctggcg
     6901 gaacgggagc agggcattac catcgatgtg gcttatcgct atttcgccac gccatcgcgc
     6961 aagttcatca ttgccgatac gcccggacac gaacagtaca cccgcaacat ggtgaccggg
     7021 gcttcgactg cggatttggc catcgtgctg gtcgacgctc gcaagggact gctgacacag
     7081 tctcgccgcc acgcctatat cgcaagcctg ctgcgcatcc cgcacgtggt gctggcggtg
     7141 aacaaaatgg acctggtgaa ctattcgcag gcggtgttcg atgagataag cacggcgttc
     7201 aatgcatttg tcaaggatct ggatttccat cgggtcgatg cgttgccaat gtctgccttg
     7261 caaggcgata tggtggtgtg gcgcggtgag aaccttccgt ggtaccaagg ccagacgctg
     7321 cttgaaatat tggaaagtgc cgaagttgcc cgttctgcgg tgcatggaga tcttttcttc
     7381 cccgtgcagt tggtgtcgcg gccgggcgat agcggcgtgg acgacgcgcg cggctacgcg
     7441 ggccggattg aatccggccg catctccgtt ggcgatgcga taaccgtgct gccttccgga
     7501 caggtttctc gggtgcgcat gatccgcact ttcgatggag ccgtcaggca ggcgcaggct
     7561 ccacaatcgg tgactgtggt gcttgcagac cagctggaca tttcgcgtgg tgaggttctg
     7621 actgcgactg cagcgccacc ggccgtggcg cgccaattcg aagccacgct gtgctggctc
     7681 gcgaccgagc cgttcaaccg cagtcgccgg tatttgatca agcatggcac ccgcacagtg
     7741 aagggactgc ttgattcgcc tcaatacaag ataaacgtga attccatgga acgcgaaccc
     7801 gccgatacct ttgcgctgaa cgagataggc cggatatcgg taaaaacggc gcagccgctt
     7861 gctgtccagc cctatcggtt caacagggct gccggcagct tcatcgttat cgatgagagc
     7921 aacaacaata ccgtggctgc cggcatcatc gaatcggcct gaaatgggca aagtctattt
     7981 tgtcggtgcg gggcccggcg ccgctgacct gattacggta cgcggcgcgc aacttctcag
     8041 acgggcggac attgtgttcc acgatgcgct ggtgcagcag gacatgctcg cgcttgctga
     8101 aaaagcaaag ctggtggctg ttggaaaacg atgcagccag atttcgactg accagcgctt
     8161 catcaaccgc aatcttgtcg aagcgtcagc caaatataac gtcgttgctc gcctgaaggg
     8221 cggtgatccg acgttgttcg gccgcctgca ggaagaaatc gacgctcttg aagcctcagg
     8281 cgtcgaatac gagattgtgc cgggcgtaac ggcggcattg gccggcagtg ccgaaatcgg
     8341 cgtatcactc acgcgaaggg gaatgagccg cagcgtggtg ttcgtcaccc ccagggtcgg
     8401 cgaaggcgag gacgaaagcg attgggcaca ggtggttgcg gcggcggaca ccgcggtgct
     8461 ctacatggct gcagggcagg cagaggccat ttcgcgcatc ctgcgccagc acggcatgcc
     8521 cgcaagcacg ccggctgtca ttgtcgtaaa tgtttccttg gctgtggcgc agcggatcca
     8581 tactgtcttg gaacggctcg aacatgatgt tccgacgcaa ctgaccggcc ctgccgtgct
     8641 catgatcgga agcgtgttcg cgtccgatcg cgcgatccgg cacgccggcg acgaagcgct
     8701 gtcactaaca gccaatttcc gctagccagg aatccccgtt tgccctggac agccggc
//
LOCUS       DATMZQ010000086         6267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2833562, whole genome shotgun sequence.
ACCESSION   DATMZQ010000086 DATMZQ010000000
VERSION     DATMZQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2833562"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VN496_02685"
     CDS             complement(<1..224)
                     /locus_tag="VN496_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXF15879.1"
                     /translation="MRILGIDPGLRVTGFGVLEIVGQKLTYVTSGRIRTSDTGELPER
                     IRTILEGLSRVIAELKPQQVAVEKVFVNINP"
     gene            complement(327..1064)
                     /locus_tag="VN496_02690"
     CDS             complement(327..1064)
                     /locus_tag="VN496_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXF15880.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKVFTRLIKEITVASRLGGSDPA
                     SNARLRLAVDKAYAANMPKDTVERATKRGSGELEGVNYEEIRYEGYGIGGAAVMVDCM
                     TDNRVRTVADVRHAFAKNGGNLGTDGSVSFLFRHCGQLMFAPGTSEEKVMEAALEAGA
                     EDVIANDDGSVEVITTPNDFFSVKDKLQKFGLKPAVAEVTMKPTTETVLSGEDGVKMQ
                     KLIDALESLDDVQEIYTSAVMDEATPG"
     gene            1197..2702
                     /locus_tag="VN496_02695"
     CDS             1197..2702
                     /locus_tag="VN496_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HXF15881.1"
                     /translation="MTRALPIAKASHELCLLPALANRHGLIAGATGTGKTVTLQTMAE
                     AFSSIGVPVFMADVKGDLAGLSQAGSMNPKLKERVDKLALPDFSFTAWPTVFWDVYGA
                     LGHPVRATVSEMGPLLLARLLNLNETQGGVLNLVFKIADDQGLLLLDLKDLRAMVQHV
                     GDNARQFTTQYGNISAASIGAIQRGLMAIDEQGGDHFFGEPALNISDLMQTDDKGRGI
                     ISILAAEKLMNAPKAYATLLLWLLSELFEQLPEVGDPEKPKLVFFFDEAHLLFTDAEP
                     VLLSKIEQVIRLIRSKGVGVYFVTQNPLDVPDTVLGQLGNRVQHALRAFTPRDQKAVN
                     TAATTLRANPALKVEKVITELAVGEALVSFLDEKGIPAVVERAFVLPPHSRMGSITMD
                     ERTATIKSSTVAGVYEQVVDRESAYEVLKERRQQAAATPASPAPGPATSSAGSIFGDV
                     LGSVFGQGNSHRQSAGEAMVKSAARAIGSEVGRQIMRGVLGSILGGTGRRR"
     gene            complement(2799..3893)
                     /gene="ychF"
                     /locus_tag="VN496_02700"
     CDS             complement(2799..3893)
                     /gene="ychF"
                     /locus_tag="VN496_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXF15882.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     EVPDPRLAKLSAIAKPLKIVPAIVEFVDIAGLVAGASKGEGLGNQFLATIRETDAIVH
                     VVRCFADDNIVHVTGKVDPLSDMETIETELALADLATVEKAVSRNRKLAQSGDKEAKL
                     LVAVLDKVQAQLDQAKPVRALHFSKEEKALINPLCLITAKPTLYAANVDEHGFGDDNP
                     LLQKVRQKAEKERAPVVAFSARMEAEIADLSEDDKRVFLSDMGLEEPGLDRLIRAGYA
                     LLGLQTYFTAGEKEVRAWTIHQGDTAPQAAGAIHTDFEKGFIRAEVISFDDYVNYRGE
                     QGAKEAGKMRLEGKEYIVKDGDVMHFRFNV"
     gene            complement(3969..4535)
                     /gene="pth"
                     /locus_tag="VN496_02705"
     CDS             complement(3969..4535)
                     /gene="pth"
                     /locus_tag="VN496_02705"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXF15883.1"
                     /translation="MKLVVGLGNPGREHEATRHNAGYWWVDKLAARTGTKLRAEARFH
                     GLAGKLMESGRECWLLKPTTYMNVSGRAVQALAQFYKIAPEDMLIVHDELDLPPGDVK
                     LKKGGGSAGHNGLKDITAHLGPDYWRLRIGIGRPLDREQMVNFVLNVPRKEELPLIED
                     AILRSLDIWPLVLDGKSEAAMLKLHTKT"
     gene            complement(4618..5340)
                     /locus_tag="VN496_02710"
     CDS             complement(4618..5340)
                     /locus_tag="VN496_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HXF15884.1"
                     /translation="MEISAMSRSLQGTGASRRLRVTGKVPGIIYGGEQPAKNVELDHN
                     GLFHQLKQESFHASILSMSVDGKKERVLLRDVQMHPWKQLVLHVDFQRVSANKQIHMK
                     VPLHFINADIAPGVKTGGGIVSHVMNEIDITCLPDKLPEFIEVDLQNLEIGAAVHLSD
                     IKIPEGVESVQLTRGDNAVVATVQIPRAMVEAEPTVEAAVEGAAVEGAAPAAAGAAPA
                     AAAPAAGAAAKKEPEKKEGGKK"
     gene            complement(5449..>6267)
                     /locus_tag="VN496_02715"
     CDS             complement(5449..>6267)
                     /locus_tag="VN496_02715"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXF15885.1"
                     /translation="ELLENVRGKDVFVLQSTCMPTNDNLMEVLLMTDALKRSSAGRIT
                     AALPYLGYARQDRRPRSARVAISAKVVANMMVVAGVDRVLTMDLHADQIQGFFDIPVD
                     NVYSSPILLGDIWKLGHQDLVVVSPDVGGVVRARALAKRLESDLAIIDKRRPRPNVAT
                     VMNIIGDVAGRTCVIMDDMVDTANTLCEAALALKEEGAQTVLAYCTHAVLSGNAIERI
                     EKSVIDELVVTDTIPLREDARQCKRIRQLSIAALLAETMLRISNEDSVSSLFME"
BASE COUNT         1248 a         1915 c         1726 g         1378 t
ORIGIN      
        1 ggattgatat tgacgaatac tttttccacg gcgacctgct gcggtttgag ttctgcgatc
       61 actcgcgaca ggccttccag gatagtgcgg atgcgctccg gcagctcacc agtgtctgaa
      121 gtacggatgc gcccgctggt cacataggtc agcttctgtc cgacgatttc cagtacgccg
      181 aatcctgtca cgcgcagacc gggatcaatg ccaagaatgc gcacgctcac ggtgtgagcg
      241 ccaatgggcg gcgaggcgca aaccacggtt gcgccgcgaa cgacggttcc gccggccggg
      301 cggtgctcaa ggccagcgcg cgcgcttcat ccgggcgtcg cctcgtccat gaccgcactg
      361 gtgtagattt cctgcacgtc gtcgagtgac tccagggcat cgatgagctt ttgcattttc
      421 acgccatctt caccgctcag cacggtttcg gtggtcggct tcatggtcac ttcggccacc
      481 gccggcttga gcccaaactt ctgcagcttg tccttgactg aaaaaaaatc gttcggggtg
      541 gtgataacct ctacgctgcc atcgtcgttg gcgattacat cttcggcacc cgcttccagt
      601 gcggcctcca tgactttttc ctcgcttgtc cccggagcaa acatcagttg tccgcaatgc
      661 ctgaacagaa aggaaaccga accatcggta ccgaggttgc cgccattttt ggcgaatgca
      721 tgccggacat cggccacggt gcgcacacga ttgtcggtca tgcaatcaac cattaccgca
      781 gcgccgccga tgccgtaacc ctcgtagcgg atttcttcgt agttgacgcc ttccagttca
      841 ccgctgccac gtttggttgc gcgctccacc gtgtctttgg gcatgttggc ggcgtaggcc
      901 ttgtcgaccg cgagccgtaa acgcgcattg cttgcaggat cggagccacc caggcgcgat
      961 gcgacagtga tttccttgat gagtcgcgta aacaccttgc cgcgcttggc atcctggcgg
     1021 cccttgcgat gctgaatgtt ggcccacttg ctgtgacctg ccatgaattc ctctgcgtag
     1081 acaactgccg ccgcgtggcg gcgtccatac ctacgcgcgt cgatcgattg gtcgcgctga
     1141 tataattatt caaaaatctt accatacggt ccgcttccgc ccgcactcaa atactcatga
     1201 ctcgcgctct gcccattgcc aaggcgtcgc acgaactgtg tctgttgccc gcgctcgcca
     1261 accgccatgg tttgatcgcc ggcgctactg gtaccggcaa gacggtgacc ttgcaaacca
     1321 tggcagaagc attcagctcc atcggcgtcc cggtcttcat ggcagacgtc aaaggtgatc
     1381 tggccggcct gtcgcaggcc ggttcaatga acccgaagct gaaagagcgc gttgataaac
     1441 tggccctacc cgatttttcc tttacggcct ggcccaccgt tttttgggac gtgtacggcg
     1501 cattgggcca tcccgtacgg gcgacggttt cagagatggg gccgctgctg ctggcgcgtt
     1561 tgctgaacct gaacgagacg cagggcggtg tactgaacct ggtattcaag atcgccgatg
     1621 accagggttt gttgttgctc gatctgaagg acctgcgcgc catggtgcag cacgtcggag
     1681 acaatgcgcg acagttcaca acccagtacg gcaacatctc ggccgccagc attggcgcga
     1741 tccagcgcgg cttgatggcc atcgatgaac aaggcggcga ccattttttc ggtgagccgg
     1801 ccctcaacat ttccgatttg atgcaaacgg acgacaaggg gcgcggcatt attagcattc
     1861 tggctgcaga gaagctcatg aatgcgccca aggcgtatgc gacattgctg ctgtggctgc
     1921 tgtcggaact gttcgagcaa ctgcccgagg tcggcgatcc ggaaaagccg aagctggttt
     1981 tctttttcga tgaagcgcat ttgctgttca ccgatgcaga gcctgttctg ctgagcaaga
     2041 tagaacaggt catccgactg atccggtcca aaggcgtggg cgtctatttt gtgacgcaga
     2101 atccgctgga tgtcccggac acggtgctgg gccagcttgg caaccgcgtg cagcacgcat
     2161 tgcgagcgtt caccccacgc gatcagaaag ccgtcaatac cgccgctaca acactgcgcg
     2221 ccaaccccgc gctaaaagtg gaaaaggtga ttaccgaact cgctgtcggc gaagccctgg
     2281 tgtcgtttct ggatgaaaaa ggcattcctg cggtagtaga gcgcgctttt gtactgccgc
     2341 cgcacagccg catgggatcg atcacgatgg acgagcgcac cgccactatc aagagttcca
     2401 cagtagccgg agtctacgag caggtagtgg atcgcgaatc ggcctacgaa gtactcaagg
     2461 agcggcgcca acaagcggcc gccacaccgg cttctccggc acccggacct gcaaccagct
     2521 ccgccggatc catatttggc gatgtactgg gtagcgtgtt cggccaaggc aattcgcatc
     2581 ggcaatccgc cggcgaagcg atggtcaaaa gcgcggcgcg tgccatcgga tcggaagtcg
     2641 gccgacagat catgcgtggc gtgctcggtt ccatcctagg cggtaccggg cgccggcgct
     2701 aacccggaaa tctctttgat ggcagctctt gtcggccacg atctttcgcg ggccttctga
     2761 aggcttagtg cacacgactg atgcgggcag attccggtct acacattgaa acggaaatgc
     2821 atgacgtctc cgtccttgac gatgtactcc ttgccttcca accgcatctt gcccgcttcc
     2881 ttggcgccct gctcgccgcg gtagttcaca tagtcgtcga atgagatcac ttcggcacga
     2941 ataaaacctt tttcaaaatc ggtgtggatg gcgccggctg cctgcggcgc ggtgtcgcct
     3001 tgatgaatgg tccaggctcg cacttccttc tcgccggcgg tgaaataagt ctgcaaaccc
     3061 agcagtgcat agcctgcgcg gattagccga tcgagacccg gttcctcaag gcccatgtcg
     3121 ctcagaaaca cgcgcttgtc gtcctcggac agatccgcga tttcggcttc catgcgcgct
     3181 gagaaggcca ccaccggcgc acgctctttc tctgcctttt ggcgcacctt ctgcagcagc
     3241 ggattatcgt ccccgaaccc gtgttcatcg acattcgccg cgtacagagt gggcttggcg
     3301 gtaatcaaac agagcgggtt gatcagtgcc ttttcctctt tggaaaaatg cagcgcgcgc
     3361 accggcttgg cctgatcaag ctgcgcctgg actttatcca gaacagcaac cagcagtttt
     3421 gcctccttgt ccccggactg agccagcttc cgattgcgcg acaccgcttt ttcaactgtc
     3481 gccagatcgg caagtgccag ctcggtttca atcgtttcca tgtcggacag cggatcgacc
     3541 ttgccggtca catgcacgat attgtcgtcc gcaaaacagc gaacgacatg gactatcgcg
     3601 tcggtttcgc ggattgtggc aagaaactgg ttacccagac cttcgccttt ggatgcacca
     3661 gcgaccagtc cagcgatgtc gacgaactcg acgatcgccg ggacaatctt cagcggtttg
     3721 gcgatggccg acaatttcgc caggcgcggg tcgggcactt cgacaatccc aacgttcggc
     3781 tcgatggtac agaaaggata attttccgcc gcaataccgg ctttggtcag cgcattaaac
     3841 agagtcgatt tgccgacgtt cggcagcccg acgattccgc atttaagaga catgtctgat
     3901 gatcggtaaa cggtgaacgg taaacagtga attccggcct gaccggatca ctattcactg
     3961 ttcgcggttc acgttttggt atgcaacttg agcattgccg cctcactctt tccgtccagc
     4021 accagcggcc agatatcgag gctgcgaagg atggcatctt caatcaatgg cagttcttcc
     4081 ttgcgcggca cgttgagcac gaaattgacc atctgttccc gatccagcgg acggcctata
     4141 ccgattctca gccgccagta gtccggaccg aggtgcgccg ttatatcctt taggccgtta
     4201 tgtcctgccg agccgccccc ttttttaagt ttcacgtccc ccgggggtaa atcgagttcg
     4261 tcgtgcacga tcagcatgtc ttccggcgcg attttgtaaa actgcgccag tgcctggacg
     4321 gcgcgaccgc tgacgttcat atatgtagtc ggtttaagca accagcattc tctgccactc
     4381 tccatcaact tcccggccaa gccgtggaag cgagcttccg cacgcaattt cgtgccggta
     4441 cgcgcggcca gtttgtccac ccaccaataa ccggcgttgt ggcgcgtggc ctcgtgctcg
     4501 cggccgggat taccaagtcc tacgaccagc ttcatgtcgg tgggcagggc gtcaaaagca
     4561 aacggcctgc cggacaggca acgctgacgg caggccgtgc tgcgaacgga tggaaaatta
     4621 cttcttgccg ccttccttct tctcgggctc cttcttggct gcggcaccag cggccggcgc
     4681 ggcagctgcg ggtgctgcac cggcggctgc tggagccgcg ccttcgactg cagcgccttc
     4741 cactgcagcc tcgacggtcg gttcggcttc caccatggcg cgcgggatct gtaccgtcgc
     4801 cacgactgca ttatcgccac gcgtcaattg caccgactcg acgccttccg ggatcttgat
     4861 atcagacaag tgaacggctg cgccgatctc aagattctgc agatccactt caatgaattc
     4921 cggaagtttg tccggcaaac aggtgatatc gatttcattc atcacatggc tgacgatccc
     4981 gccgccggtc ttcacgccag gcgcgatatc ggcattgata aagtgcagcg gaaccttcat
     5041 gtggatttgc ttgttcgccg atacgcgctg gaagtcgacg tgcaggacca actgtttcca
     5101 cgggtgcatt tgcacgtcgc gcaacagcac gcgttctttt tttccatcca cgctcatgct
     5161 gaggatcgat gcgtgaaagg attcctgctt gagttggtgg aacaacccgt tatggtctaa
     5221 ctccacgttc ttcgcaggtt gctctccgcc gtaaataatg cccggcacct tgcccgtaac
     5281 gcgcagacgg cggctcgcac ccgttccctg cagcgaccgg gacattgcac tgatttctat
     5341 cgttgccatg tgactctcca atgtgaggcg gcaaaccgcg accagctgcc acctgctcta
     5401 ataaagaggt ggcattgcag ccacctcgct caaaaaagta tcgttaagct attccatgaa
     5461 caatgaggaa accgaatcct cgttgctgat gcgcagcatc gtttctgcga gcagtgctgc
     5521 gatgctcagc tgacgtatgc gcttgcactg tcttgcatcc tcacgcagcg gaatggtatc
     5581 ggttaccacc agttcgtcga tcacggactt ttcaatgcgc tcgattgcat taccggacaa
     5641 caccgcatgt gtacagtaag cgagcacggt ctgcgcgccc tcttctttga gcgcgagtgc
     5701 cgcctcgcac agcgtgttcg cggtatccac catgtcatcc atgatcacgc aagtgcggcc
     5761 cgcgacatcg ccgataatgt tcatgaccgt cgcgacattg ggccgcggac ggcgcttgtc
     5821 gatgatcgcc agatcggatt cgaggcgctt agccagcgct cgcgcacgca ccacaccgcc
     5881 tacgtccggc gaaacgacga caagatcctg atgtccgagc ttccagatgt cccccagcaa
     5941 aatcggggac gagtaaacgt tgtccaccgg aatatcgaaa aatccctgta tctgatcggc
     6001 atgcagatcc atcgtcaaca cgcgatccac ccccgcgacc accatcatgt tcgcgactac
     6061 cttggcactg atcgccacac gtgccgaacg cggccgccgg tcctgccgcg catagcccag
     6121 atagggaagc gccgcggtaa ttcgtccggc agacgagcgc ttcagcgcat cggtcatcag
     6181 caggacttcc atcaaattat cgttggtcgg catgcaagtc gactgcaaca caaaaacgtc
     6241 tttgccgcgc acgttctcca gcagctc
//
LOCUS       DATMZQ010000087         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4179482, whole genome shotgun sequence.
ACCESSION   DATMZQ010000087 DATMZQ010000000
VERSION     DATMZQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4179482"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1032
                     /locus_tag="VN496_02720"
     CDS             316..1032
                     /locus_tag="VN496_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF15886.1"
                     /translation="MRDRVLIVDDDEDIQSLLEAYLRKNGFDAQAVGDGKTMWEALAA
                     KPVSMVVLDLMLPGEDGLSLCRQLRQRSQVPVLMLTARGEAVDRILGLEMGADDYLPK
                     PFDPRELVARIRSILRRAKSLPADTETNVPETFSFMGWRLDTRARNLCAPDGVVVPLS
                     GAEYRLLLIFLQNPNIVLSRDQLSNFTYGRDADPLDRTLDMQVSRLRERLREQAREPE
                     IIKTVRGKGYVLATRVEATP"
     gene            1029..>2240
                     /locus_tag="VN496_02725"
     CDS             1029..>2240
                     /locus_tag="VN496_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF15887.1"
                     /translation="MRLLPRSLFGRIVLILAGGFLAIQLITTAIAISDRNALVFRAGA
                     NQAATRVGDVVRALTAATPSERTRIMHAISGDTLKVTYGKPAGSDAAAAEESELTLTA
                     RDALALALQPGIGFKMIDARPVYLNPDSWYAREFGERPGVRMYAAVLLNDGVWITVES
                     IDPPRTTFWITRMFRNLAIVDSVMVILCFFAVRVVTRPLSILASAAEDLGRNIDRPPL
                     PETGANELVRASRALNVMQDRLKRYVDTRIKVLAAMSHDLKTPLTRMRLRSEMLDDAH
                     IKGKFTKDLDELQQMVGSTLDYMRGLSEGTEALQPIDVSALLSSLKDDAQEAGHTVTV
                     SGDARNPVMGRTQALKRCLQNLVDNALAYGRRADITLREEGGALHIAIRDDGPGIPES
                     DIEKVFEPFYRV"
BASE COUNT          458 a          645 c          696 g          441 t
ORIGIN      
        1 cgcttttggc aaacacgaag taggctgctc cccccaggat gccaagcacg agcacccata
       61 gccaccgccg tcgccaccat gccgcgtggg aggacggatg ctgcgatgga gtcagcacat
      121 cgtcttgcgt ggacgaatga accgcgttgc tcatcaactg tgattcagga aggccatgag
      181 taattttcgc aagggcgaac aactgtatgt catgccccca tcgcctgtgt aacgaaatgt
      241 cggaagccgc gccagggtta cagtaagtga caaaacttct cccaataggt ttcggtcgga
      301 ggtataagag cagccatgcg agaccgtgta ttgattgttg acgatgatga agacatccag
      361 tcgctgctgg aggcgtacct gcgcaagaac gggttcgacg cgcaagccgt tggcgacggc
      421 aagacgatgt gggaagcgct ggccgcgaaa cccgttagca tggtggtgct ggatttgatg
      481 ctacccggag aggatggact ctcgctgtgc cgccagttgc ggcagcgttc gcaggtgccg
      541 gttctgatgc tgactgctcg cggggaagcc gtcgatcgga ttctcgggct cgaaatgggg
      601 gccgacgact atcttcccaa accattcgac ccacgggaat tagtggctcg cattcgcagc
      661 atcctgcggc gcgcgaaatc gctgcccgcg gataccgaaa ccaatgtgcc ggaaacgttc
      721 agcttcatgg gatggcggct cgatacccgc gcacgcaatc tgtgtgcgcc ggatggtgtc
      781 gtggtgcctt tgtccggcgc ggaataccgg ctgttgctga tttttctgca gaatccgaat
      841 atcgttctgt cgcgcgacca gctttccaat tttacttacg gacgcgatgc cgatccgctc
      901 gatcgtaccc tggacatgca ggtaagccgt ttgcgcgagc gtctgcgcga gcaggcgcgc
      961 gagcccgaaa tcatcaagac ggttcgcggc aaggggtacg ttctggcgac acgcgtggaa
     1021 gcaacaccat gaggctgctg ccacggtcct tgttcggacg cattgtgctg attctggcgg
     1081 gcggcttcct ggcgatccag ttgattacca ctgcgatagc aatttcggac cgcaacgcgc
     1141 tggtgttccg cgccggcgca aaccaggccg ccacgcgagt cggggatgtg gtgcgggcat
     1201 tgacggcggc cactcccagc gaacgcacac gcatcatgca cgcgatttcg ggcgatacgc
     1261 tcaaagtcac ctatggcaaa cccgcgggct cggacgccgc tgcagcagag gaatcggaac
     1321 tcacgctgac cgcgcgcgat gcgcttgcac tggcgttgca gccaggtatc ggtttcaaaa
     1381 tgatcgatgc gcgcccggtt tatctcaatc ccgattcctg gtacgcgcgg gaattcggag
     1441 agcgccctgg cgtgcgcatg tacgcggcgg ttttgctcaa tgacggagta tggattaccg
     1501 tggagagcat cgacccgccc agaaccacat tctggattac ccgcatgttc cgcaacctgg
     1561 caatcgtcga tagcgtgatg gtgatcctgt gcttcttcgc cgtgcgcgtc gtcacccggc
     1621 ctctgtcgat tctcgccagc gcagcagaag atctcggccg caacatcgat cgtccgcctt
     1681 tgccggagac gggcgccaat gaactggtgc gcgcgtctcg tgcgctcaat gtcatgcagg
     1741 accgcctaaa gcgctacgtc gatacgcgca tcaaggtgct cgctgcaatg tcccacgacc
     1801 ttaagacgcc cctcacacgc atgcgcctgc gctcggaaat gctggacgat gcgcatatca
     1861 aggggaagtt caccaaggat ctcgacgaac tgcagcagat ggtcggctct accttggact
     1921 atatgcgcgg actatcggaa ggcacggaag cactgcagcc gatcgacgtc agcgcgctgc
     1981 tgtccagcct gaaagatgat gcgcaggaag ccgggcatac ggtgaccgta tcgggagacg
     2041 cgcgcaatcc ggtcatgggc aggacgcagg cgctgaagcg ttgtttgcag aatctggtgg
     2101 acaatgcgct ggcgtatgga aggcgtgcgg acatcacgct gcgcgaagaa ggcggcgcgc
     2161 tgcatatcgc gatcagggat gacggtcccg gaattccgga atcggacatc gaaaaggtat
     2221 tcgagccgtt ctatcgcgtc
//
LOCUS       DATMZQ010000088         2512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3178357, whole genome shotgun sequence.
ACCESSION   DATMZQ010000088 DATMZQ010000000
VERSION     DATMZQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178357"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VN496_02730"
     CDS             complement(<1..450)
                     /locus_tag="VN496_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF15888.1"
                     /translation="MEQVLLRGGNVWDGSGKPAFAADVLIAGNHIAEILPHGSPGPAA
                     AMPIDIPGHTVIPGMVDGHAHLPFLHKADMADTGDVPPEEHTLRTVQHAATILQAGFT
                     ACVSGGSAKPRLDIAVRNEINAGHIPGPRILAASPELTNTGNLGDERR"
     gene            583..1341
                     /locus_tag="VN496_02735"
     CDS             583..1341
                     /locus_tag="VN496_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF15889.1"
                     /translation="MEGKFLQGKVAVITGASRGLGRAMALALGASGAKLALVGRNRDK
                     LDETRKLVAELGAESEILVADVRNEEQVAKLEKDVIARFGSTHILINNAGTAVRKNIA
                     DFSLDEWKLVLDTNLTGVFLVSRAFIPHMKKAKWGRIINMTSIMAHIGSTGRGAYCAS
                     KAGLLALTKCLALELVGDGITVVAISPGFYETDLTAPLRADAQKNAALMTATPAGRWG
                     KPNEIGSLARYICTDATSFMTGTDILMDGGWVAQ"
     gene            1388..>2512
                     /locus_tag="VN496_02740"
     CDS             1388..>2512
                     /locus_tag="VN496_02740"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF001933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HXF15890.1"
                     /translation="MKIVVLGAGVIGVTTAWYLTQDGHDVTVLDRQPQPGLETSFANG
                     GQISVSHAEPWANPGAPAKILKWLGREDAPLLFRLRADPRQWIWGVRFLLECLPARTQ
                     RNTLTILRLALYSRQLLKALRRESGVQYDQLEKGILQIHTDEREFEAAHARVDLLRSH
                     GCEMHIKSVPQCEELEPALAASTIRLIGGTYAPDDESGDAHQFTGELAELCKQRGVQF
                     GFEHAIEALTTEGDRVIGAVVRDPEGRRELVKADSFVVALGSYSPFLLSPVGISVPIY
                     PVKGYSVTVALTHPEKAPMMCLSDENAKVAISRLGSRLRAAGTAELTGYDTSLSAPRC
                     NAILSRIEQLFPGAGDYAKASAWAGLRPATPSNVPLIGRTG"
BASE COUNT          512 a          742 c          792 g          466 t
ORIGIN      
        1 ccggcgctcg tcaccaaggt tgccggtatt ggtgagttca ggactggcgg ccagtattcg
       61 cgggcccggg atgtgcccgg cgttaatttc attgcgcacg gcaatgtcga gtcgcggttt
      121 tgcggacccg ccgctgacac aggcagtaaa tcccgcctgc agaatggttg cggcatgctg
      181 cacggttcgc aaggtatgtt cttcgggcgg cacatccccg gtgtccgcca tgtcagcctt
      241 gtgcaggaac ggcaaatgcg cgtgcccgtc aaccatgccg gggatcacgg tgtgacccgg
      301 aatgtctatg ggcatggcgg cggccgggcc cggcgacccg tgcgggagga tttcggcaat
      361 gtggtttccg gctatcagca catcggcggc aaacgcaggt ttgccgctgc catcccagac
      421 gttgcctccc cgcagcagga cttgctccac tgtccctcca gattttgcgc aggcaaaaat
      481 tgtaaccgcg actgcaggga catagtggac caatgtgcag gtaaaaggca caagtggcgc
      541 gggttatact gggccagtca caaaaacaac tggggagatc gcatggaagg caagttcctg
      601 caagggaaag tcgcagtcat cacgggggcg agccgcggac tgggccgggc catggcgctc
      661 gcacttggcg catccggtgc aaaactggct ctggttggcc gcaatcgcga caagcttgat
      721 gaaacgcgca aactcgtggc ggaacttggc gcagaaagcg aaatcctggt cgccgacgtg
      781 cgtaatgaag aacaggtggc caagcttgag aaggatgtca tcgctcgctt cggcagcacg
      841 catattctga tcaataacgc cggaaccgcg gtacgcaaaa acatcgctga tttttctctg
      901 gacgaatgga agctcgtcct ggacaccaac ctgaccggcg tattccttgt ttcacgtgca
      961 ttcatcccgc atatgaagaa agcgaaatgg ggccgcatca tcaacatgac ctcgatcatg
     1021 gctcatatcg gcagtaccgg ccgcggcgcc tattgcgcat ccaaggctgg attgctggcg
     1081 ctgaccaagt gcctcgcgct ggagctggtc ggtgatggca tcacagtggt ggcgatcagc
     1141 ccaggattct acgaaaccga tttaacggca ccccttcgcg ccgacgcaca aaaaaatgcc
     1201 gcgctgatga ccgcaacgcc cgccgggcgt tggggaaaac caaatgaaat cggttcgctt
     1261 gcgcgctaca tctgcaccga tgcgacctcg ttcatgactg gcaccgatat cctcatggat
     1321 ggaggatggg tcgcacagta aagctgccag gccgtcacga catgtcggtt cgcggcagag
     1381 taccagcatg aaaatcgtcg tcctcggtgc cggcgtaatc ggcgtgacga ccgcctggta
     1441 tctgacgcag gacgggcacg atgtcacagt gctggaccgg caaccgcagc ccgggctcga
     1501 gaccagtttc gcgaacgggg gacaaatctc ggtgagtcat gccgagcctt gggcaaaccc
     1561 gggtgcaccc gccaagatcc tcaaatggct cgggcgcgaa gatgctccgc tgctgttccg
     1621 attgcgtgct gacccgcgtc agtggatctg gggcgtgcga tttctgcttg aatgcctgcc
     1681 ggctcgcacg caacgcaata cgcttaccat cctgcgcctg gcgctctaca gccggcaatt
     1741 actcaaagcg ctgcgccgcg agtcgggcgt gcagtatgac cagctcgaaa aaggcatcct
     1801 gcagatccat accgatgagc gcgagttcga ggctgctcat gcgcgcgtcg atctgctgcg
     1861 cagccatggc tgcgagatgc acatcaagag cgtgccgcag tgcgaggagc tcgagccggc
     1921 cttggcggca tcgaccattc ggctgatcgg cggtacctat gctccggacg acgaatccgg
     1981 cgatgcccac cagttcaccg gcgagctggc ggaactttgc aagcagcgcg gcgtgcagtt
     2041 tggcttcgag cacgccatag aagcactgac caccgaaggg gaccgggtga ttggagccgt
     2101 ggtccgcgat ccggagggtc gccgggaact agtcaaagcc gacagttttg tcgtggcact
     2161 cggcagttac agcccatttt tattgtcccc ggtgggcatt agcgtgccga tctatccggt
     2221 caaagggtat tcggtcaccg tagcgctgac gcatccggag aaggctccaa tgatgtgcct
     2281 gagcgacgag aacgccaaag tcgccatttc aagattgggg tcacgcctgc gggccgccgg
     2341 cacggctgaa ctgaccgggt acgacacctc gctgagcgcg ccgcgctgca acgcgatcct
     2401 gtcccggatc gagcagcttt tcccgggcgc cggagactat gccaaggcgt cggcatgggc
     2461 tgggctgcgt ccggcaacgc cgagcaacgt gccgctgatc gggcgtaccg gg
//
LOCUS       DATMZQ010000089         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3869910, whole genome shotgun sequence.
ACCESSION   DATMZQ010000089 DATMZQ010000000
VERSION     DATMZQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869910"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /locus_tag="VN496_02745"
     CDS             <1..1682
                     /locus_tag="VN496_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF15891.1"
                     /translation="ITEAKGERIESVFMVVDETTREEAFDSIEMVLRTGRAVSMSSNV
                     ILMKRGGKEILINKSAAPIHDRSGQVTGIVLVFHDVSREGQYAARLSYQASHDALTGL
                     INRHEFEHHLSQALKSATRFRRSHSLLYLDLDQFKVVNDTCGHAAGDDLMRRIGALIR
                     ARLGKEAIVARLGGDEFGVLLENSPLEHATQVAEDLRQSINDFRFSWMAMPFTVGASI
                     GVVCIAEGTFTMEEVLRAADAACYMAKEKGRNRVNVYRADDTEVSSRQGEMEWVTRIQ
                     RALDENRLRLFSQQIISLRQPSKHNTHCEVLLRMLDEQGELVPPARFIPAAEKYGLMP
                     AIDRWVVTNAFNSYASLAAKSRALPIQTCSINLSGKSINEDSFLEFLREQATIAGVPY
                     SAFCFEVTETAAVENLPKAVNFIEQLRALGCRFSLDDFGVGMSSFGYLKHLPVDYLKI
                     DGGFVKDMLEDPIDKAMVEAINDIGHVMGKKTIAEFAENTAIVDVLREMGVDYAQGFG
                     VAKPVPFGAAPGLALTPRSFGDKSASIRQRDTDERDTAKGNASTAKAEGSD"
     gene            complement(1975..3150)
                     /locus_tag="VN496_02750"
     CDS             complement(1975..3150)
                     /locus_tag="VN496_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HXF15892.1"
                     /translation="MAILAIAAISGCGGGGGGSSNGMLGVSLTDAPACGFDAVNVTVS
                     KFRVHMNAAAPDSAGGWSEITLNPPRKINLLDLTNGLLEDLGETPLPAGHYTQMRLVL
                     EANTGSNPLANSVIPTGSAETALKTPSATQSGIKLVNPFTVASGQRADFVLDFDACKS
                     VVKNGNGNYLLKPVIKIVPNTANGIDGFVQPALISGNNVVVSAQVNGEVVASTVPAST
                     GKFFLARLAPGTYDVVVSAKGHATAVIATVPVADATSVVTLSTSTAPIVLPTSAVSHS
                     ISGTVLLNPVNADQVIFVSAKQDVLPATILVPATTVTVRAQSADLTTGAYSLLLPAGA
                     PLLGQFGALPITLDAQTNPAGKYVIEASATGYATQTSFEENVSTSDQPNVNLTLLVP"
BASE COUNT          686 a          905 c          949 g          668 t
ORIGIN      
        1 cgatcacgga agccaaaggc gagcgcattg aatcggtatt catggtggtc gacgagacca
       61 cacgcgaaga ggcattcgat tcaatcgaaa tggtattgcg cacggggcgc gcggtatcga
      121 tgtcgtcaaa cgtcatcctg atgaagcgcg gcggcaagga gatcctgatc aacaagtcgg
      181 cggcgccgat tcacgatcgt tccgggcagg taaccggtat cgtgctggta ttccacgacg
      241 tcagccgcga aggtcaatat gcggcacgac tgtcctatca ggcgagccac gatgcgctga
      301 ccggattgat caatcggcac gaattcgagc atcacttgag tcaggcgctg aagagcgcga
      361 cgcggtttcg ccgaagccat tcgctgctgt acctggatct cgatcagttc aaagtcgtga
      421 acgatacctg cggacatgcc gcgggcgatg acctgatgcg caggatcggc gcactcattc
      481 gcgcccgcct tgggaaagaa gcgatcgttg cgcgactcgg aggcgacgag ttcggcgtgc
      541 tgctggaaaa ttctccgctc gaacatgcaa cccaggtcgc agaggatctg cgccagagca
      601 tcaacgattt tcgtttttcg tggatggcca tgcctttcac cgtgggcgcc agcatcggcg
      661 tagtatgtat tgccgaaggc acgttcacca tggaagaagt gctgcgcgcg gctgatgcgg
      721 cgtgctacat ggccaaggaa aaagggcgca accgcgtcaa cgtgtaccgt gccgacgaca
      781 ctgaagtgtc atcgcgccag ggcgagatgg aatgggtcac gcggatacag cgcgcgctgg
      841 atgaaaatcg cctgcgcctg ttctcgcagc agatcatcag cctgcgccag ccttcgaaac
      901 acaatacgca ctgtgaagtg ctgttgcgca tgctggacga gcaaggtgaa ctggttccgc
      961 ccgcgcgctt catcccggca gcggaaaaat acggcctgat gcccgccatc gaccgctggg
     1021 tcgtcacgaa cgccttcaat agctatgcaa gcctcgccgc aaagagccgg gctttgccga
     1081 tccagacgtg cagcatcaat ctttcgggca agtcgatcaa cgaggatagc ttccttgaat
     1141 tccttcgcga gcaggccacc atcgcgggcg tcccgtatag cgccttctgc tttgaagtaa
     1201 ccgaaacagc ggccgtcgaa aatctgccga aagcggtcaa tttcattgaa cagttgcgtg
     1261 cactaggctg ccgcttttca ctggacgatt tcggcgtcgg catgtcgtcg ttcggttatc
     1321 tgaagcacct tccggtggac tatctgaaga tcgatggtgg tttcgtcaag gacatgctgg
     1381 aagacccgat cgacaaggcg atggtggagg cgatcaatga tatcggccat gtgatgggca
     1441 agaaaaccat tgcagaattc gctgagaaca ccgccattgt cgacgtgctg cgtgaaatgg
     1501 gtgtggatta cgcacagggc ttcggcgtcg caaaaccggt gccgttcggc gcagcgcctg
     1561 gtctggcgct cacgccgcgc tcatttggag acaagtctgc gagtatcaga cagcgcgata
     1621 cagacgagcg cgacacggcg aagggcaacg cttctaccgc taaagcggaa ggctcggatt
     1681 aagaaagcgc gctgctgtcg tgacggtcgt gcccccttcg gggattccca agggatcaca
     1741 catggtgtac cggctttcgc cataaccggt ctggcgacca taaccgggct gccgcccata
     1801 acggtcgtga ctacgtaact gctgagatta atgacccaag cggtctagtc ttgctacgtt
     1861 ccggtcttat tgctgtcatc aaccagaagc gccgctccca acaaaacgtc tcccgccgta
     1921 cgaccacaaa aaagggctgc cctgtgcagc cctctttcat tgtgccggca gctttcaggg
     1981 caccaggagg gtcaagttga cgttgggctg gtcggacgtc gacacgtttt cttcaaaaga
     2041 ggtttgcgtc gcgtatccgg ttgcggacgc ctcgatgacg tacttgcccg ccggatttgt
     2101 ttgagcgtcc agagtaatgg gcaaagcacc aaactgtccc agcaacggtg cgccggctgg
     2161 caacaacaaa ctgtacgctc cggttgtcag gtctgcggac tgtgcccgca cagtcaccgt
     2221 ggtcgctggt acgaggatcg tggcgggtaa cacatcttgc ttggcagaaa cgaaaatcac
     2281 ttggtctgca ttgaccgggt tcaacaatac ggtgccactg atgctgtgcg aaacagcgga
     2341 tgtcggcaac acgatcgggg ctgtgctggt actcagcgta acgacactgg tggcatcggc
     2401 taccgggact gtcgcaatga ctgcagtcgc atggcctttg gcactgacca cgacatcgta
     2461 tgttccgggt gccagtcgag cgagaaaaaa ttttccggtc gatgcaggga cggtcgatgc
     2521 gaccacctcg ccgttgacct gcgcggaaac gacgacgttg tttcccgaga tcagcgccgg
     2581 ctgcacaaac ccgtcgattc cgttggccgt attcgggacg atcttgatga ccggtttcag
     2641 cagataattt ccattgccgt tcttcacaac cgacttgcag gcgtcgaaat ccagcacgaa
     2701 gtcggctcgc tggccggaag cgacggtaaa cggattgacc agcttgatgc ccgactgggt
     2761 tgcgctgggc gtcttcagcg ccgtttcagc tgatccggtc gggataacgg aattcgccaa
     2821 aggattcgat ccagtattcg cctccagcac gagacgcatc tgggtgtaat gtcctgccgg
     2881 cagtggcgtc tcgcccagat cttcgagcag gccgttggtg agatccagca gattgatttt
     2941 ccgcggtgga ttcagcgtga tttcagacca gcctccagcg gagtccgggg cggccgcgtt
     3001 catgtgtacc ctgaatttgc tgaccgtgac attgaccgcg tcgaatccgc acgcgggtgc
     3061 atccgtcaaa gagaccccga gcattccatt ggaagagcct cctcctccgc cgccgcagcc
     3121 gctaattgcc gcgatggcaa ggattgccat caaccagcgt attggcgcca gtttgttgtc
     3181 atacaggttc atatcgttct cctcttct
//
LOCUS       DATMZQ010000090         3014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3870527, whole genome shotgun sequence.
ACCESSION   DATMZQ010000090 DATMZQ010000000
VERSION     DATMZQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3870527"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="gpmA"
                     /locus_tag="VN496_02755"
     CDS             <1..606
                     /gene="gpmA"
                     /locus_tag="VN496_02755"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HXF15893.1"
                     /translation="TFDLAHGSVLKRAIRTMWIVLEELDLTWIPQQLSWRLNERHYGA
                     LQGLNKSDMAERFGAEQILAWRRSYDIPPPALEKGDPRYPGNDRRYADMVPQDIPVTE
                     CLKDTVARFLPYWNDVIAPQVKSGKRVLIAAHGNTIRALVKYLDNVSEKDIVGLNIPT
                     GIPLVYELNDSLQPVRNYYLGDQEKAREAAAAVAAQARGKS"
     gene            633..1808
                     /locus_tag="VN496_02760"
     CDS             633..1808
                     /locus_tag="VN496_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXF15894.1"
                     /translation="MLRAAAVSLYLATITLNAQGAPTDELKRIRDRIEALQKQLSESE
                     SSKSEAADALKDSERAVSQTSRKIYEISGQRREVNSALSRLQSRIRVLESNIADEQAV
                     LGHALYQQYISGQSDTIKLVLNGEDPNEIARELHYLSYVSRARAELLASLRGNLADLE
                     KMTGETRRKSAELHALQVEETVQMRRLEKEKAERQQIYAKVSDDIDNSRKQLSTLKRD
                     EERLSQLIERLAREAAARKKTRTPGKHITNRALPNADTGSGAFRQLRGKLRWPVIGEL
                     TNRFGGQRQDSGLSWKGLFISSKPGRDVKAVASGRVVYADWLRGFGNLLILDHGDGYM
                     SLYGNNESLLKQVGQEIRSGDTIAATGNSGGNPDSGLYFELRFQGKPFDPLPWVTLK"
     gene            1847..>3014
                     /locus_tag="VN496_02765"
     CDS             1847..>3014
                     /locus_tag="VN496_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXF15895.1"
                     /translation="MHNKFRQVGLIAVGAILGVLISLNFSAVAQKSAGPLPIEDLRAF
                     TEVFGRIKNDYVEPVEDKKLISEAINGMLSGLDPHSAYLDVEAFRDLQVGTQGEFGGL
                     GIEVGMEDGFVKVVAPIEDTPAWRAGLKSGDLIIKLDEANVKGMTLNDAVKRMRGKPN
                     TPITLTFVRKGDAKPRVVTLTRAVIQIQSVKSKLLEPGYAYFRVTQFQEHTGETLAKA
                     ADKMFKDNAGPMKGIILDLRNDPGGLLNGAVAISSAFLSTESLVVYTEGRTEDAKMKL
                     FARPEYYLRGSKDDYLKKLPKEMKTLPMVVLVNSGSASASEIVAGALQDHKRAVVMGT
                     QTFGKGSVQTILPLGNGTAIKLTTARYYTPNGRSIQAKGITPDIVVEEATLTEGE"
BASE COUNT          746 a          791 c          879 g          598 t
ORIGIN      
        1 acgttcgacc tggcccacgg ttccgtactc aagcgcgcca ttcggacgat gtggatcgtg
       61 cttgaggaac tggatttgac ctggattcca caacagcttt cctggcgact gaatgaacgc
      121 cactacggcg cgctgcaagg actcaacaaa agcgatatgg cggagcgctt cggagcggaa
      181 cagatccttg cgtggcggcg cagttatgac atccctccac cggctttgga gaaaggcgat
      241 ccccgctatc ccggaaatga tcgacgctat gccgatatgg tccctcaaga tattcccgtc
      301 actgaatgcc tcaaagatac cgtcgcgcgt ttcctgccat attggaatga cgtgatcgcg
      361 ccccaggtga agtcaggtaa acgcgtgctc attgccgcac acgggaatac cattcgcgcg
      421 ctggtgaagt accttgataa cgtttcggag aaagacattg ttgggttaaa catcccgacc
      481 ggcatccccc tggtttatga attgaacgat tcccttcagc ctgtgcgcaa ctattacctt
      541 ggcgatcagg agaaagcgcg ggaggccgct gctgccgtag cagcgcaggc acgcggcaag
      601 tcttgagcaa agcgggaaac agcagttctc gattgcttcg cgcggccgcc gtctcgctgt
      661 acctggcgac catcacactc aatgctcaag gcgcgcctac cgatgaactc aaaagaatcc
      721 gtgaccgtat cgaggcgttg caaaagcaat tatcggaatc ggagtcgtcg aaatcggaag
      781 cggccgatgc cttgaaagat tcggagcgag ccgtcagcca gacgagccgc aagatttacg
      841 aaatttcagg acagcgtcgt gaagtgaata gcgcactatc gcgcctgcaa agccggatcc
      901 gcgtcctgga aagcaacatt gccgatgaac aggcagtgct tggacatgcg ctttatcagc
      961 aatacatcag cggccagtca gacacgataa aacttgtcct caatggcgag gatcccaacg
     1021 aaatagctcg cgagctgcac tatctgagtt acgtgtcccg cgcgcgtgcg gaactactgg
     1081 caagcttaag aggaaatctg gccgatcttg aaaaaatgac cggtgaaacg cgtcgcaaga
     1141 gcgccgaact gcacgcgctg caggtcgaag aaacggttca aatgcggcgt cttgaaaaag
     1201 agaaggcgga acgccagcaa atttacgcca aggtttcgga cgacatcgac aatagccgca
     1261 agcagttgtc aaccctcaag cgagacgagg aaaggctgtc acagttgata gagcggttgg
     1321 ccagggaagc ggcagcacgc aaaaaaactc gtacacccgg taaacacatt accaatcgtg
     1381 ccctgccgaa cgccgatacg ggttcagggg cattccggca attgagaggc aagctgcgat
     1441 ggccggtaat cggggaactg acgaatcggt ttggcggaca aaggcaggac agcggtttgt
     1501 cctggaaggg tttgttcatt agcagcaagc ctggccggga tgtcaaagca gtggccagcg
     1561 gtcgtgtcgt ttacgcggac tggctgcgag gcttcggtaa tctgctgatt cttgaccacg
     1621 gcgacggtta tatgagcctt tacggcaata acgagtcgct tctcaaacaa gtcggtcagg
     1681 aaatcaggag cggcgatacg attgccgcaa ccggtaacag cggtggcaac cctgattcag
     1741 gtttatactt tgaacttcga tttcagggaa agcctttcga ccccctccct tgggtaacat
     1801 tgaaatgaga tcgcgcattg tagtgcgcgc atgaggagtc atgaaaatgc ataacaaatt
     1861 tcgacaagtc gggctgatcg cggtgggtgc gatccttggc gtactgatca gcctgaactt
     1921 ctcggcggtg gcgcaaaaaa gtgccggtcc gcttccgatc gaggatttgc gggcattcac
     1981 ggaagttttt ggccgtatca aaaacgatta cgtggagccg gtggaagaca agaagctgat
     2041 ttccgaagca atcaacggca tgctttcggg actcgatccc cattccgcct atctcgatgt
     2101 agaagcgttc cgtgacctgc aggtcgggac gcagggagaa tttggcggcc tcggcatcga
     2161 agttggcatg gaagacggct tcgtcaaggt ggtcgcgcca atcgaagata cgcccgcctg
     2221 gcgcgccggg ctcaagtctg gcgatctgat catcaagctg gacgaagcca atgtgaaagg
     2281 catgacgctc aatgatgcag tcaagcgcat gcgcgggaaa ccgaacacgc ctatcaccct
     2341 gacttttgtg cgcaagggtg acgccaagcc gcgagtcgta acgctgaccc gtgccgtaat
     2401 ccagattcag agcgtcaaat cgaagttgct tgagccgggc tatgcgtatt tccgggtcac
     2461 gcaatttcag gagcacacgg gcgagacgtt ggccaaagct gcagacaaga tgttcaagga
     2521 caatgccggg ccgatgaaag gcatcatcct ggatttgcgc aatgacccgg gcggcttgct
     2581 caatggcgca gtggcgatct cctcggcttt tctgtcgacc gaatcactgg tggtctacac
     2641 ggaaggtcgc accgaagacg ccaagatgaa gttgttcgcg cgccccgagt attacctgcg
     2701 tggcagcaag gatgactatc tcaagaagtt gccgaaggag atgaaaacgt tgcccatggt
     2761 tgtactggtg aacagcggat cggcttctgc atcggaaatc gtcgcgggtg ccctgcagga
     2821 tcacaagcgg gcagtcgtga tgggaacgca aaccttcggc aagggatcgg ttcagaccat
     2881 tctgccactt ggcaacggca cggcaatcaa gcttacgacg gcacgctatt acacgcccaa
     2941 tggccgttcc attcaggcga agggcatcac acccgatatc gttgtcgaag aagcgacgct
     3001 gactgaagga gagg
//
LOCUS       DATMZQ010000091         3391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2109517, whole genome shotgun sequence.
ACCESSION   DATMZQ010000091 DATMZQ010000000
VERSION     DATMZQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109517"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VN496_02770"
     CDS             <1..261
                     /locus_tag="VN496_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15896.1"
                     /translation="ADLHATVYDEGGTPLTDAVIQDAAFGANAIAYRIQATTTLFSLN
                     WNRVLASGVAVNLGYAYRRSDTDNDIGGYYANLFNLSVTYSQ"
     gene            258..911
                     /locus_tag="VN496_02775"
     CDS             258..911
                     /locus_tag="VN496_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HXF15897.1"
                     /translation="MSIRSIALLAISAWLCAASPVIAADLHATVYDEGGTPLTDAVVV
                     AYPDGFAIEVPKTSTEVEDQIKKEFVPYVKPVHVGTLVKFPNKDDISHHVYSFSPAKR
                     FELPLYRGTVASPVMFDKTGIVKLGCNIHDWMIGYLYVTDAPFFAKTERQGRVDILQM
                     PAGPYRVHVWHPRMFASEDSTIRHITVQESASVEWRLKLNREFRARRTPLPDEPGYR"
     gene            1314..1790
                     /locus_tag="VN496_02780"
     CDS             1314..1790
                     /locus_tag="VN496_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXF15898.1"
                     /translation="MTLKLTSSSFAHEGQIPQIYTCEGQDVSPPLAWSGVPETAKTLV
                     LIVDDPDAPDPAAPKTTWVHWVLYNIPASATGLPQGVSPEQLPPGTLQGISDFGRTGY
                     GGPCPPIGRHRYFHKLYALDIVLADLAHPRKQDLEKAMKGHILEQAVLIGTYQKGR"
     gene            1806..2210
                     /locus_tag="VN496_02785"
     CDS             1806..2210
                     /locus_tag="VN496_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HXF15899.1"
                     /translation="MGLFSGKRPADLGVNNGALKAPPSSPNAVSSQATGGYHRIAPLT
                     YEGAQAQAMEKLKAIIESTPGTQIVQTKPDYLYAEYASALLGFVDDVEFYFPQGTHVI
                     HVRSASRLGHSDLGVNRKRIEAIRARLLQSGA"
     gene            2182..2568
                     /locus_tag="VN496_02790"
     CDS             2182..2568
                     /locus_tag="VN496_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15900.1"
                     /translation="MHDSSNPEREEKPGAEIDLSEDLRPWWRRSPSLAACAGLAIGLA
                     TLWFGNMLGRVPLDAHSTLGYLLMLPLLFIVPAFSATGIRLAWLTWSRGRPLRSWLST
                     LALAAIAANLLALAKFASALVRIFSY"
     gene            2752..3012
                     /locus_tag="VN496_02795"
     CDS             2752..3012
                     /locus_tag="VN496_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15901.1"
                     /translation="MDKKPLMQSKPVISHAGDYEEGSARRSTEQHSAAPLIENAADYI
                     DALTRPRSDNSYVHKKGIVGRRMRDKYRMNGVVVTEVVKKIA"
BASE COUNT          762 a          986 c          925 g          718 t
ORIGIN      
        1 gcagacctgc atgcgacggt atatgacgaa ggcggcacgc ctctcaccga tgcggtgatc
       61 caggacgctg cctttggagc aaacgccatt gcgtatcgga tccaggcgac gaccacccta
      121 ttttcactga actggaaccg cgtgctggcc tctggcgtgg ctgtcaattt gggctacgcc
      181 tatcgcagaa gcgataccga taacgacatc ggcggctact atgcgaacct gttcaatttg
      241 agcgtaacct acagccaatg agcatcagga gcattgcatt actggcgata tcggcgtggc
      301 tctgcgccgc ttcaccggtc atcgcggcag acctgcatgc gacggtatat gacgaaggcg
      361 gcacgcctct caccgatgcg gtggtagtgg cgtaccccga tggcttcgca atcgaagttc
      421 cgaagacgtc gacagaagtg gaagaccaaa tcaagaagga attcgtgccg tacgtaaagc
      481 cagtgcatgt tggaacgctg gtaaaatttc ccaacaagga cgacatcagt catcacgtct
      541 attctttctc cccggccaag cgcttcgaat tgccactcta caggggaacg gtcgcttcac
      601 cagtgatgtt cgacaagaca ggcatagtga agctcggctg caatatccat gattggatga
      661 tcggctacct ctatgtcacc gatgcgcctt ttttcgccaa aacggaacgg caagggcgcg
      721 tagacattct ccaaatgccc gcgggtccct accgcgtgca tgtatggcac ccgcgcatgt
      781 tcgcgtcgga agactcaacg atccgccata tcacggttca ggagtccgcc agcgtcgaat
      841 ggcggctcaa gcttaaccgc gaattcaggg cgcggcgcac gcccttgccc gacgagcccg
      901 gctaccgatg aattggttcc ttaccacgga acacggcagc gttcacggct tgcgaaattg
      961 cgcaaaaagc cttttgcctt gtacattccc cttttaaaga taggttagaa atttccatgg
     1021 cgatcgaagt caccaagaaa ctgcgtcccg acgccggctg catgcggtgt ggcagtgctt
     1081 ataccggctc cgttgaacac acagtcatgt gtttccggct caacaacaac gggatgccct
     1141 gtaacggcag aattacctgg cggccgaatc ctgaagattg gaccgagtgc cccgtctgcg
     1201 ccggtacggg tctgaacgat aacggacgct gcggtcgctg ccatggcgac ggttggctga
     1261 tgggaaaaca ttagcgcggt tgctcgcggt tcatgcgata gcgcaacacg gccatgacgc
     1321 tgaaattaac ttcatcatcc ttcgctcacg aaggtcaaat tccacaaatt tatacatgcg
     1381 aaggccagga cgtctctccc ccgcttgcat ggagcggtgt tccggaaacg gcaaaaactc
     1441 tggtgctaat tgtggatgat ccagacgcac ccgatcctgc cgcaccgaag acgacgtggg
     1501 tccactgggt gctctataac attccagcct cggcgaccgg attgccgcaa ggcgtatcgc
     1561 cggaacaatt gccgcccggc accctgcagg gcatcagcga tttcggtcgc accggctacg
     1621 gtggcccatg tccgccaatc ggccgccacc gctatttcca caaactctat gctcttgaca
     1681 tcgtgctagc cgacctcgcg cacccgcgca aacaggacct ggaaaaagcg atgaagggac
     1741 acattctcga gcaagccgta ttgataggaa cttaccaaaa gggccgctga actggcgtaa
     1801 acgcgatggg actattcagt ggcaaacgcc cggccgatct cggtgtgaac aacggagccc
     1861 tcaaggcgcc cccgtcttca ccaaacgcgg tcagtagcca ggcgacgggc ggctaccacc
     1921 ggattgcgcc attgacctac gaaggcgcgc aggcacaggc aatggaaaaa ctgaaagcaa
     1981 ttatcgaaag caccccgggt acgcaaatcg tgcagacaaa gcccgactat ctgtatgccg
     2041 aatatgcgag tgccctgttg ggcttcgtgg acgacgtgga attttatttt ccccaaggaa
     2101 cgcacgtgat ccatgtgcgc tcggcttctc gcctgggcca cagcgatctc ggggtcaacc
     2161 gcaaacgcat agaagccatc cgtgcacgac tcctccaatc cggagcgtga ggaaaagccg
     2221 ggcgcggaaa tcgacctgag cgaagacctc aggccctggt ggagacgctc accatcactc
     2281 gctgcgtgcg ccgggctggc gatcgggctg gcgacactgt ggttcgggaa tatgttgggc
     2341 cgggtgccgc tcgatgcgca ttcgacgttg ggctatttgc tgatgcttcc gctcttgttc
     2401 atcgtcccag cgttctcggc gactggaata agactcgcct ggctgacatg gtcacgtggg
     2461 cgtccgctgc gatcctggtt aagtacactg gcgctggctg caatagctgc gaacttgctc
     2521 gcgcttgcaa aatttgcaag cgcgttggtg cgcattttca gctattaaag caagcggcaa
     2581 cgccgcccct tccatcggat acaaaccaaa ttattcactt aagcgcctga tcgttaaatc
     2641 agttttgcat caggatcgca tggtcaacaa cctgtaacat acgcgcgacc aacggtgtgc
     2701 tagcgtatgg ctacttctcg acgacctaaa cttgcctatg gatactagcc tatggataaa
     2761 aaacccttga tgcaatcaaa gccggttatc tcccatgcgg gggattacga agaaggttct
     2821 gctcgccgca gcactgaaca acattccgcc gcgcctctca tagaaaatgc agctgactat
     2881 atcgatgcac tcacgcgccc gcgttccgat aattcatatg tccataagaa gggcatcgtc
     2941 ggccgtcgga tgcgcgacaa atacagaatg aacggcgtag tcgtgaccga ggtggtcaag
     3001 aaaatcgcct gagtaactcc aggccgccga agggcgcact atgatgcgcc ctttttattt
     3061 tgagctgttg ggttcgctgt ttgcctattc tattcgcggc tctcgcggcg cttttcagtt
     3121 gcttgttcac caaatcggtg ccactctttg cgcgtttggt gcaatcccgg cattgcaatg
     3181 cggacgtaaa gggacgaccc cccgcggccg gccgtgcgtc cgatccattg ccaattccgc
     3241 acgttaacgg ctttaccatc gtgagaaccg gcgcaggcat gttctgtgcg aacggagttc
     3301 gctgcagacc gtgtcaaccg caggccaatg tcatgagcat cgtgcctttc ttcttcgacg
     3361 tgctggccta cagctcgatc ttcgtgctgg t
//
LOCUS       DATMZQ010000092         5752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1802552, whole genome shotgun sequence.
ACCESSION   DATMZQ010000092 DATMZQ010000000
VERSION     DATMZQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802552"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..301)
                     /locus_tag="VN496_02800"
     CDS             complement(17..301)
                     /locus_tag="VN496_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15902.1"
                     /translation="MKVFAIVFAAAFALLTNAYAGDPEFGGQCTMGMAEGRKMQTNCS
                     VLWLGSDDKIYCFADQAAKQKFLQAPKENLKRAQAFWEDPENLKRLIKRE"
     gene            complement(374..1303)
                     /locus_tag="VN496_02805"
     CDS             complement(374..1303)
                     /locus_tag="VN496_02805"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXF15903.1"
                     /translation="MKIAVVGCGAMGSVYAALLGDAGHEIWAIDSWKEHVEAMKAHGL
                     HLEGASGDRTVRVNATTQAADAGLCDLVILATKAMHVEKAARSMQPLLKPDTVVLSIQ
                     NGLGGPDTAAKVLGRERVLVGVVGGFGASMKAPGHAHHNGWELVRLGELAGPISPRLE
                     AVAEIWRSGGFRVKCFDDIDQLIWEKLICNVCFSGTCAITQRTIIEVIEDTDAWQVAS
                     SCATEAFNVARARGIKLDFTDPVTYVRAFGMKIPNARPSMLLDHMAGRMSEIDAINGA
                     IPLAAKAAGLNAPSNEVISALVRAKERSMGVRQ"
     gene            1523..2554
                     /locus_tag="VN496_02810"
     CDS             1523..2554
                     /locus_tag="VN496_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF15904.1"
                     /translation="MEYRQLGGSGFKVPALSLGTGTFGGGNEFFKSWGASDVAEATRL
                     VDICLEAGLTMFDTADVYSGGLAEEILGQAIKGRRNQLLISTKGTFRTGPGPNDLGSS
                     RFHLTEAVEASLRRLGTDHIDLYQLHGFDARTPVEEVLLTLDNLVRAGKIRYIGCSNF
                     SGWHLMKSLAVSEKYGLARYVAHQAYYSLVGRDYEWELMPLALDQKIGTVVWSPLGWG
                     KLTGKLRRGQALPPTTRLQSKLSNDKGPPVANEQVYKVVDALDEIAGQTGKTVPQIAL
                     NWLLQRPSIASIIVGARNEEQLRQNLGAVGWQLTANQIARLDAASATTLAYPYWHQRG
                     FAELNPPPV"
     gene            2612..3790
                     /locus_tag="VN496_02815"
     CDS             2612..3790
                     /locus_tag="VN496_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXF15905.1"
                     /translation="MRPLDGVTVVALEHVIAGPFCTRQLADLGARIIKIERPGVGDPA
                     RAYDERVRGLSSHFVWTNRSKESITLDLKREEARGIVYKLLENADVLVQNLAPGAAAR
                     MGFSYDALKDRYPRLIVCDISGYGADGPYRDKKAYDLLIQSESGLLSVTGTPDTPSKA
                     GCSIVDIASGMYAYTNILAALIQRGRTGTGSRIDVSMLESMAEWMSFPLYYAYEGAAP
                     PTRAGASHATIYPYGPFPAGDGKIVMLGLQNEREWAVFCDKVLEKPELKADPRFSSSS
                     RRSAARKELYQIIVEALSHLTAAQLVERLDDAQIGNARMNDMHEVWEHAQLRARGRWK
                     DVDTPAGKIKAMLPPGVPESFQPRMDPIPDIGQHTEAILSELGYDKTTIAGLRAGGVI
                     "
     gene            3787..4614
                     /locus_tag="VN496_02820"
     CDS             3787..4614
                     /locus_tag="VN496_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXF15906.1"
                     /translation="MSLMAMNRPVERSYLYVPGNRPERFDKACAAGADAVIVDLEDAV
                     PPVEKDSARAALSSWLAADKSVLVRINAVGSEWFEGDLAACDVQGISGVILPKAEGVA
                     DAVISLCRRRRIALFPLIETAAGMAQCEAIAATPCVLRLMFGTIDFQFELGIEGDGDE
                     LLAFRSRLVLASRIAGIQSPVDGPCTSWEDPALLSSDCQRARRLGFGGKLCIHPKQVA
                     IVNAAFAPGESEIAWAHKVLDAAQRSGGAAVAVDGRMIDRPVILKAEQIIREATRSV"
     gene            4663..5682
                     /locus_tag="VN496_02825"
     CDS             4663..5682
                     /locus_tag="VN496_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HXF15907.1"
                     /translation="MAANTMRALQANDFSIDALCISEKPVPKPRRGEILIRVKAASLN
                     YRDLAILVQKYLPTLPLPYVPASDACGEILEIGEDVTRFKVGDRVIPVYTQGWHDGLP
                     TVELRTRRTLGAPLSGVLQDYVVVPAEDAVSVPAHLSDGEAATLPIAALTAWSTLQEG
                     NIKPGDTVLVQGTGGVALFALQLARLAGASVIALSSSDDKLARAKQLGAAIGINYRTT
                     PDWHVAVREATQGKGVDIIVETAGATLDKSLASLAFGGFIGVVGFVAGYKAEISIRAL
                     LGPMIRVQGIAVGSRARFEAMNRAIAQHKLKPVIDSTFSLEQSADAFMHMQQGKHFGK
                     IVIAL"
BASE COUNT         1160 a         1673 c         1728 g         1191 t
ORIGIN      
        1 cctttttttt gcgctcctat tcgcgcttga tcagcctctt cagattctcc ggatcttccc
       61 agaacgcctg cgcacgcttc aggttttctt tcggcgcctg caaaaatttc tgctttgctg
      121 cctgatcggc aaagcagtaa atcttgtcat ccgacccaag ccacagtacg gagcaattcg
      181 tttgcatttt tcttccctcg gccatgccca tggtgcattg gccgccaaat tccgggtcgc
      241 cggcataagc atttgtgagc aatgcaaacg cagccgcaaa gacaatggca aaaactttca
      301 tgcgaatcct tttggaaaaa acgttggtag gattttacag gaggaagagt ccggtccccg
      361 catccagttt tggctattgc ctcacgccca tcgagcgctc tttcgcgcgt acgagtgcgc
      421 taatcacttc gttagagggc gcgttgagcc cggctgcttt tgctgccagc ggaatcgcac
      481 cattgatggc atcgatttcc gacatcctgc cagccatatg atcaagcagc atcgatggcc
      541 gtgcattcgg gattttcatg ccaaatgcac ggacgtacgt caccggatca gtgaagtcga
      601 gcttgatgcc gcgcgcccgc gccacgttga atgcctcggt cgcgcaactt gaagcaacct
      661 gccaggcatc cgtatcctcg atgacctcga taatcgtgcg ctgtgttatt gcacaggtgc
      721 ccgagaagca gacgttgcaa ataagctttt cccaaatcag ctgatcgatg tcgtcaaagc
      781 atttcacgcg aaacccgccg gagcgccaga tctccgccac ggcttcgagc cgtggcgaaa
      841 tgggccctgc cagttcgccc aggcgaacca gctcccagcc gttgtgatgc gcgtggcccg
      901 gtgctttcat cgatgcgcca aagccgccga ctacgccgac gagcacacgt tctcgcccca
      961 gaaccttggc cgccgtgtcg ggaccgccaa ggccattctg gattgacagc acgacggtat
     1021 ccggcttcag cagcggctgc atcgatcgcg ctgccttttc gacatgcatg gctttggtcg
     1081 ccaggatcac cagatcgcaa aggcctgcat ccgctgcctg cgtcgtcgca ttgacgcgca
     1141 cggtgcgatc tcccgacgcg ccttcgaggt gcaagccgtg ggccttcatg gcttcgacat
     1201 gttccttcca gctgtcgatc gcccagatct cgtgaccggc gtctcccagc aaggctgcat
     1261 agacgctgcc catggcacca caaccgacca ccgcgatttt catgcgcgct ttccagaaca
     1321 tcgacatccg attctaacca actccggtcg ttctttggcc atagcgtggt taagcgtctc
     1381 cgcatgcggt tgcattacaa tccgggctca ccgaacgagc cgcgcccagc aatgcgaacg
     1441 ccaacaaggg caaaacgcct tgcgtggctc ccctgtgaac tttttttaac gcggttttgg
     1501 ccgatggccg gatggaggac gcatggaata caggcagttg ggcggttcag gtttcaaggt
     1561 tccggcgttg agcctgggca ccgggacttt cggcggaggc aatgaattct tcaaatcatg
     1621 gggcgccagc gacgtagcag aagcaacgcg gctggtcgat atttgtctcg aagcgggcct
     1681 gaccatgttc gatactgccg acgtctattc cggcggtttg gccgaagaaa ttctgggaca
     1741 ggcaatcaag ggcaggcgca atcaactgct catttcaacc aaaggcacgt tccgcacggg
     1801 gcctggaccg aatgaccttg gctcctcccg ttttcacctg accgaagcgg tggaagcaag
     1861 cctgcggcgg ctggggacag atcatatcga cctgtaccag ctgcatggct tcgatgcacg
     1921 cacgccggtc gaagaagtct tgttgacgct cgataatctg gtccgtgcgg gcaaaatccg
     1981 ttatatcggt tgctcgaatt tctcaggctg gcatctgatg aaatcgctgg ccgtttctga
     2041 aaaatatggc ttggcgcgtt atgtcgccca tcaggcgtat tacagcctgg tcgggcgaga
     2101 ttacgaatgg gagctcatgc cgctcgccct cgaccagaag attggcaccg tcgtctggag
     2161 tccgctcggc tggggcaagc tgactggaaa actccgaaga ggacaggcgc tgccgccaac
     2221 cacccgtctg cagagcaagc tctcgaatga caagggaccg ccggttgcca acgagcaggt
     2281 ctacaaggtg gtcgatgctc tggatgaaat tgccggccaa accggcaaaa cggtgccgca
     2341 gattgcgctg aactggctgc tgcagcggcc cagtattgcc agcatcatcg taggtgcgcg
     2401 caacgaagaa cagttgcgcc agaatctggg cgcggttggc tggcagctga cagcgaacca
     2461 aatcgccagg ctcgatgccg ccagcgccac cacgctggct tatccatatt ggcatcaacg
     2521 tgggttcgcc gaactcaatc cgccgccggt atgaggggcg gggctctgca aatatattta
     2581 cgggaacccc agtgactgag gactggtatc catgcgtccg cttgatggag tgacggttgt
     2641 ggcgctggaa catgtcatcg ctggtccgtt ctgtacgcgc cagctcgccg atctgggtgc
     2701 gcgcatcatc aagatagagc gtcccggcgt gggcgatccg gcgcgcgctt acgacgagcg
     2761 tgtgcgtggc ctgtcgtcgc atttcgtctg gacgaaccgt tccaaggaaa gcatcacgct
     2821 cgatctcaag cgcgaagagg cgcgcgggat cgtatataag ctgctggaga atgcagatgt
     2881 gctggtgcag aaccttgcgc ccggcgcggc tgcccgcatg ggattttcct atgacgcgtt
     2941 gaaggaccgt tatccgcgcc tgatcgtctg cgatatctcc ggctatggcg ctgacgggcc
     3001 atatcgcgac aagaaggcct atgacctgct gattcaaagt gaatcaggat tgttgtccgt
     3061 caccggcacg ccggacacgc cttcgaaggc gggatgctcc attgtggata ttgcctccgg
     3121 catgtacgcc tacacgaaca tccttgctgc gctgatccag cgtgggcgga ctggcaccgg
     3181 cagccgcatc gacgtgtcca tgctggaaag catggccgag tggatgagct tcccgcttta
     3241 ctacgcctac gagggagccg caccgcccac gcgtgcgggc gcctcgcacg cgactattta
     3301 tccttacgga cctttcccgg ccggtgacgg caagattgtc atgcttggac tgcagaacga
     3361 acgggagtgg gcggtgtttt gcgacaaggt gctggaaaag ccagaactca aggctgatcc
     3421 gcgattctca tcgagttcac gccgttcggc cgctcgcaaa gagctctacc agattatcgt
     3481 cgaggcgtta tcgcacctga ccgccgcgca acttgttgaa cggctcgacg acgcgcagat
     3541 tggcaacgca cgcatgaacg acatgcacga agtctgggag cacgcgcaac tgcgcgcacg
     3601 cggccgttgg aaagacgttg acactccggc aggcaagatc aaggcgatgt tgcccccagg
     3661 ggtccccgag agttttcagc cgcgcatgga tccgattcct gacattggcc agcataccga
     3721 ggccattctg agtgaactcg gctacgacaa gacgaccata gcagggctgc gtgctggtgg
     3781 ggtgatatga gcctcatggc catgaaccgg ccggttgagc gctcgtacct gtatgtaccg
     3841 ggtaaccggc cagagcgttt tgacaaggcc tgtgcggccg gagcagatgc cgtcatcgtc
     3901 gatcttgagg atgccgtgcc gcccgtcgaa aaggattccg ctcgcgcggc actgagttcg
     3961 tggttggctg ccgataagag cgtcctggtt cggatcaatg ctgtcggctc cgaatggttt
     4021 gagggcgatc ttgcggcatg cgatgtccaa ggcatcagcg gcgtgatcct gccgaaggcc
     4081 gagggcgtcg ctgacgctgt gatttccctg tgccggcgca ggcgcatcgc gcttttcccg
     4141 ctgatcgaaa cggcagcagg catggcgcag tgcgaagcga tcgccgccac accgtgcgta
     4201 ctgcgcctga tgtttggcac cattgatttc cagttcgaac tcggtatcga aggcgacggg
     4261 gacgaattgc tcgcgttccg gtcgcgtctg gtgctggcct cgcgcatcgc aggcattcaa
     4321 tcaccggtcg acgggccttg tacgtcatgg gaggatccag cgttgctgag ttcggactgc
     4381 cagcgggcgc ggaggctcgg tttcggcggg aaactgtgca tccatccaaa acaagttgcc
     4441 atcgtgaatg cagcgtttgc accaggcgag tcggaaatcg cgtgggcaca taaagtgctc
     4501 gacgcggcac aacgttcggg cggcgccgcc gttgccgtcg atggcagaat gatcgaccgg
     4561 ccggtcattc tcaaggctga gcaaattatc cgcgaagcga caagaagcgt gtaggtgacg
     4621 gatgttccgc tgccatacca acagatcaaa cagcgaggcg taatggcagc gaataccatg
     4681 cgggcgttgc aagcgaacga tttctcgatc gatgcattgt gcatttcgga aaaacccgtg
     4741 ccgaaaccac ggcgcggtga gatcctgata cgtgtcaagg ccgccagtct gaactatcgt
     4801 gatctggcga ttctcgtaca gaagtacctg cccacgttac ctctgcctta tgtgcctgcc
     4861 tcggatgcct gcggggaaat cctggaaatt ggcgaagatg tgacccggtt caaggtaggc
     4921 gatcgcgtca tacccgttta cacgcagggc tggcacgacg gtttgccgac cgtggagtta
     4981 cgcaccagac gaactttggg cgcacccttg tccggggtgt tgcaggatta cgtcgtggta
     5041 cccgcggagg acgcggtttc tgtgcccgcg catctcagcg atggcgaagc ggcgactctg
     5101 ccgatcgcgg cgttgacggc ctggtcgacg ctgcaggaag gcaatatcaa gcccggtgac
     5161 accgtgttgg tgcaaggcac gggcggcgtg gcgctgttcg cgctgcaatt agcacgcctc
     5221 gccggtgcaa gcgtgatagc gctctcatcc agcgatgaca aactggcacg cgcgaagcag
     5281 ttgggcgcgg caatcggcat caactatcgc acgacgccgg actggcatgt ggccgtgaga
     5341 gaagctacac agggcaaagg cgtcgatatc atcgttgaaa cggccggagc caccctcgac
     5401 aaatcgctcg cgtcgcttgc ctttggcggc ttcattggcg tggtgggctt cgttgccggg
     5461 tacaaggcgg agatttcgat ccgtgcattg ctcggcccga tgattcgcgt gcagggcatt
     5521 gcagtcggtt cgcgcgcaag attcgaagcc atgaatcgtg cgattgcgca acacaagctg
     5581 aagcccgtca tcgattccac gttttcactg gagcagtctg ccgatgcatt catgcacatg
     5641 cagcaaggca agcacttcgg caaaattgtg atcgcgctgt gagcgggccc gtgatgcaac
     5701 tggagcaatt gcgcgcctgg atcggccgta ccgaaacacg gacggacctg gc
//
LOCUS       DATMZQ010000093         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2493027, whole genome shotgun sequence.
ACCESSION   DATMZQ010000093 DATMZQ010000000
VERSION     DATMZQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493027"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..927
                     /gene="moeB"
                     /locus_tag="VN496_02830"
     CDS             139..927
                     /gene="moeB"
                     /locus_tag="VN496_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXF15908.1"
                     /translation="MDDNQLLRYSRHILLPEIEINGQQKLLDAHALIVGAGGLGAPVS
                     LYLAASGVGRLTICDDDDVDLTNLQRQIVHFTDSIGERKVESARRTLARINPQTVVET
                     IAERVAGEKLEQLVQAADVILDCSDNFGTRHAINRACVQFSKPLVSGAGVRFDGQVTV
                     FDLRKADSPCYHCLFPMGGDVEEMRCAIMGVFAPLVGIIGSVQAAEALKLLIGVGRNL
                     TGRLLLLDALAMEWRSVRLKKDPDCPVCGARAMAQTDSSALQER"
     gene            complement(933..1400)
                     /locus_tag="VN496_02835"
     CDS             complement(933..1400)
                     /locus_tag="VN496_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15909.1"
                     /translation="MHIARLIKVLLLACAAALVSGCGASDDEFAKVVVGTWGSREVAP
                     DDVIVENRFTLLPGGRVNWQGELRMLVPPDFYLPDRPNYEVRNGRLVYYFSASGKWSV
                     HDGYLHTKIESSTLPSLLPAGFAAAWKLKEVTSKEMIYVSAANGRTRVEFREQ"
     gene            complement(1406..1978)
                     /gene="slmA"
                     /locus_tag="VN496_02840"
     CDS             complement(1406..1978)
                     /gene="slmA"
                     /locus_tag="VN496_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HXF15910.1"
                     /translation="MPTKPGERKLQILQTLATMLENPKVEKITTAALAARLDVSEAAL
                     YRHFASKAQMFEGLIEFIEQTLFGLINKITGEETSGLKQLEAIISSLLGFAKKNPGMT
                     RVLIGDALVNEDERLQARINQLQDRLEATLKQAVRFAVSEKEIPENTDAAGQANLMIA
                     FVIGRWHQFAKSGFRRDPTEHWAAQWPALV"
     gene            complement(2063..2719)
                     /locus_tag="VN496_02845"
     CDS             complement(2063..2719)
                     /locus_tag="VN496_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HXF15911.1"
                     /translation="MSISGAAKSRVWIFDLDNTLHDARPHIFPHINRSMTDYVARLLD
                     LGDEQASALRDAYWKRYGATLIGLIRHHGVNPAHFLRETHRFPDLGGMIVGRGELRHV
                     LRRLPGRKLVFSNAPSAYAGAVLRALGVHDLFDGVFAIEHTRFRPKPEAHAFLRLMRA
                     HRLIASRCVMVEDSLENLRTARKLGMKTVWVTESMCAPGWVNARVRHLIRLPGLLRLL
                     "
     gene            complement(2738..>3421)
                     /gene="argB"
                     /locus_tag="VN496_02850"
     CDS             complement(2738..>3421)
                     /gene="argB"
                     /locus_tag="VN496_02850"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXF15912.1"
                     /translation="GPQIDDMLKRIGKKGEFVQGMRVTDRETMDIVEMVLGGLVNKDI
                     VNLINHNGGRAIGLTGKDGCFIRAKKLLLQNTLDDGEMLDIGQVGAIQSIDPEVISLL
                     ESREFIPVVAPIGVGEDGESYNINADLVAGKLAEILHAEKLILLTNTPGVLDKDGKLL
                     TGLTPKQVEKLFADGTIHGGMLPKIGSALDAAKSGVKSVHIIDGRVEHALLLEVLTDQ
                     GVGTLIRNR"
BASE COUNT          723 a         1002 c          968 g          728 t
ORIGIN      
        1 gaatcaggcg ttaaatctgc tcaagggctt gcagattatc ggcaagcgat agagccgccg
       61 acgcaaactg aagcaacgga aggcccgcct agtgcgggcc ttccgcgcta agatgcccgt
      121 aaattgcttt tccagcccat ggacgacaac cagttactgc gctatagccg ccacattttg
      181 ttgcccgaga tcgaaatcaa cggccaacaa aagctgttgg atgcgcatgc cttgatcgtc
      241 ggggcgggag gattaggtgc gccggtatcc ctttaccttg cagcaagcgg ggtcgggcgg
      301 ctcacaattt gtgacgatga tgacgttgac ctgactaatc tgcagcggca gatcgtgcat
      361 ttcaccgatt ccatcggtga acgcaaagtc gaatcggcgc ggcggacgtt agcccgcatt
      421 aacccgcaaa cagttgttga aaccattgcc gaacgcgtgg cgggcgaaaa actggaacaa
      481 ctggttcagg ccgccgatgt catcctggat tgcagcgaca acttcggcac gcgccatgcg
      541 atcaaccggg cttgcgtgca gttttcgaaa cccctcgttt caggcgcggg agtacgcttc
      601 gacggacaag tcaccgtatt cgatctgcgc aaggcagaca gcccctgtta tcactgcctg
      661 tttccaatgg gcggggacgt cgaggaaatg cgctgcgcga tcatgggggt gtttgctccg
      721 ttagtcggca tcattggatc agtccaggcc gcggaagcgc tcaaactgtt gataggcgtg
      781 ggccggaatc ttaccgggcg gttattgctg ctggatgccc tggccatgga atggcgcagc
      841 gtgagactca agaaagaccc ggactgtccg gtatgcggtg cacgagccat ggcgcaaacg
      901 gatagttctg cgcttcaaga gcgctagcag ctttattgct ctcgaaattc aacgcgcgtg
      961 cgcccattcg cggcggacac gtagatcatc tccttgctgg tcacttcctt gagcttccat
     1021 gctgccgcaa aacctgccgg cagcaaactc ggcaacgttg aagattctat ttttgtgtgc
     1081 aggtagccgt cgtgtactga ccacttgccg ctggcggaaa agtagtacac caaccggcca
     1141 ttccggactt cgtagttggg ccggtccggc aagtagaaat cgggcggaac aagcatgcgc
     1201 aattcccctt gccagttgac ccgcccgccg gggagcaatg tgaatcggtt ttcaacgatc
     1261 acgtcatccg gcgccacttc ccgcgagccc caggtgccaa cgaccacttt tgcaaactcg
     1321 tcgtccgaag caccgcaacc actcaccaat gcagctgcac acgcgagcaa aagtaccttg
     1381 attaaccgcg cgatgtgcaa cggcactaga ccagcgccgg ccattgtgcg gcccaatgtt
     1441 cggttgggtc gcgtctgaat ccgctcttgg cgaattggtg ccagcggccg atcacaaaag
     1501 caatcatcag gttcgcctgc cctgccgcgt cggtattttc gggaatctct ttctcggaga
     1561 cggcgaagcg cacggcctgc ttcagtgtcg cctccaggcg gtcctgcagt tgattgatac
     1621 gcgcctgcag gcgttcatcc tcattgacca atgcatcgcc aatcagaact cgcgtcatgc
     1681 cagggttctt ttttgcaaac ccaagcaagg aggatatgat cgcttcaagc tgcttcagtc
     1741 cgctggtttc ttccccggta attttgttga tcaggccgaa cagcgtctgc tcgatgaatt
     1801 caatcaagcc ctcgaacatc tgtgccttgc tggcgaaatg ccggtagagc gcggcctcgg
     1861 agacatccag ccgggcggca agcgccgcgg tcgtaatctt ctccaccttg ggattttcaa
     1921 gcatggtcgc aagggtctgc agaatctgca gcttgcgttc tccgggcttc gtcggcatgg
     1981 cggcgctttg tggtgggaag accaaaggtt acccgaacgg cgcggaattt aacacgaacc
     2041 ggtgtgatgc gaaccgactg gttcaaagaa gccgcaatag gccaggcaat cggatcaggt
     2101 gtctcaccct tgcatttacc catcccgggg cacacatcga ttcggtgacc cacacggtct
     2161 tcattcccaa tttccgtgcg gtacgcaggt tttccaggct gtcttcgacc atgacacacc
     2221 gcgacgcgat cagacgatgc gcgcgcataa gccgcaaaaa ggcgtgtgct tccggcttgg
     2281 gccgaaagcg agtgtgctca atggcaaaaa caccgtcgaa caagtcatgc accccgagtg
     2341 cgcgtagcac agcacccgcg taagccgaag gtgcattgga gaacacgagc ttgcgcccag
     2401 gcaggcgccg cagaacgtgg cgcaattcgc cgcgaccgac gatcatcccg ccgagatcgg
     2461 gaaagcgatg cgtttcacgc aaaaaatggg cggggttgac gccgtgatga cgtatcaagc
     2521 cgatgagcgt tgcgccatag cgcttccagt aagcatcgcg taacgcactg gcctgctcgt
     2581 cgcccaagtc cagcagcctg gcaacataat cggtcatcga tcggttgatg tgcgggaaga
     2641 tatgcggccg cgcatcatgc agggtgttat caagatcgaa tatccagacc ctggatttgg
     2701 ccgcccctga aatactcata tcggctctgg atgcctgcta gcgattgcgg atcagcgtcc
     2761 cgacaccttg gtcggtcagt acctccagca gcagcgcgtg ctcgacgcgc ccgtcgatga
     2821 tatgaacact tttgacgccg ctcttggcgg cgtccagcgc agagccgatc ttgggcagca
     2881 tgccgccatg aattgtgccg tccgcgaata gcttttccac ctgttttggc gtgagcccgg
     2941 tcagcagctt gccgtccttg tcgagtactc ccggcgtatt ggtcagcagg atcaacttct
     3001 ccgcatgcag gatctcagcg agtttgccgg caacgagatc ggcattgatg ttgtaagact
     3061 cgccgtcttc accgactccg atgggggcaa cgaccggaat gaactcgcgg ctctccagca
     3121 aggaaatgac ttccggatcg atgctctgta tggcgcccac ctggccgata tcaagcattt
     3181 cgccgtcgtc gagcgtgttc tgcaacaaca gttttttggc acgaatgaaa cagccatcct
     3241 tgccagtcag accgatggcg cggccgccgt tgtggttgat caagttgacg atgtccttgt
     3301 tgaccaggcc acccagaacc atttcgacga tgtccatggt ctcgcggtcc gtcacacgca
     3361 tgccctggac gaattccccc ttcttgccga tacgcttgag catgtcgtca atctggggcc
     3421 c
//
LOCUS       DATMZQ010000094         5207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4873184, whole genome shotgun sequence.
ACCESSION   DATMZQ010000094 DATMZQ010000000
VERSION     DATMZQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4873184"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VN496_02855"
     CDS             <1..1293
                     /locus_tag="VN496_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HXF15913.1"
                     /translation="FVTALEIAALDHMRMVAAIAPFVDSAISKTVNVPENYPFEDFRD
                     LYFEAWKAGLKGITTYRPNGVLGSVLSVTPASEAPNDLDTSDADRRIRLDAAPMPALS
                     SLRWPGRPQMTAGNPSWTYMVESPFGAFAIFVGHVDNGDPHAFEVWVNGNEQPRGLGA
                     VAKTLSMDMRAQDRAWVRTKLDILSRTAGDDAFDCPMPPDGAPVRMPSTVAAFARIVS
                     WRVEQLGGLTPRDDRSPVLDALFAKKEPKTGTDGTMSWSVDVLNRSSGDDFVLILKEL
                     VLPSGQRRPYSVWMAGSYPRSLDGLCKLLSLDMRVIDPAWIGMKLRKLLSYAEPLGDF
                     MAKVPGEDKQRNWPSTVAYVAQLMIHRFAMLGVLDERGYPIDEMGVLEIPQAARARAA
                     RSLQILPGKRCLECGNSAVIRKDGCEFCTACGAVGACG"
     gene            complement(1447..2847)
                     /locus_tag="VN496_02860"
     CDS             complement(1447..2847)
                     /locus_tag="VN496_02860"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXF15914.1"
                     /translation="MSALAKHNAVNAPSAVTLSGSTALAGPITAADVRIKDLGKCLLP
                     SPVVAHLGESAVMFAGAAEKVLVDDRMSVFGDNVARAGNVPAFELAGPRDKVFFDPRT
                     VGCGIVTCGGLCPGINNVVRGLVLELARGYGVKRILGFRFGYEGLISRFGHDPVTLTA
                     ESVAHIHHQGGTILGSSRGSQDPGEVIDNLEANGINILFVIGGDGTIRGAMHFTEEIE
                     RRGLKIAVVGVPKTIDNDIHFIDRSFGFESAYSAAVDVVRSARVEAMGARNGIGLVKL
                     MGRHSGFVACQAALASADVDLVLIPEVNAMLEGERGVFANLDRVLARKGHAVVVVAEG
                     AAQELMTDDAADGATDKSGNAKLKDIGVYLRERITEHYRQAKREITLKYIDPSYTIRS
                     VPATPSDSVYCWNMARNAVHAAMAGNTEMLIGRWHGRFVHVPMPLATRSRKQVDVTAD
                     LWMSVIEATGQPRSFA"
     gene            3234..4649
                     /locus_tag="VN496_02865"
     CDS             3234..4649
                     /locus_tag="VN496_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXF15915.1"
                     /translation="MSTTFEALGLRAELVRAVADEGYTEPTPVQEQAIPVILAGRDIL
                     AGAQTGTGKTAGFALPLLQLLAARHAGGDAHRRPIRALILTPTRELAAQIEDSVRTYG
                     RHLRMKSTTVFGGVSMNQQIGALRRGVDILVATPGRLLDHLQSRTVDLSRVEILVLDE
                     ADRMLDMGFVRDIRRILMTLPAKRQNLFFSATFSSEIRELADSFLHAPVQVAVAQSTV
                     TADLISQVVHPVDKDRKRAMLEHLVNTGDWRQVLVFARTKHGANRLAEQLQKNGISAD
                     AIHGNKSQGQRTRALAQFKQGKVRVLVATDIAARGLDIEQLPHVVNYELPNVPEDYIH
                     RIGRTGRAGSSGQAVSLVCSEERDFLRDIERLLKRSIERVVVEGFQPTGAPLRTDSHS
                     PASRERRPQQRSQSDRPRTQGRDVRTGSGEGRRSQGNAGRPARNGGEGRQRSGASARR
                     GDSQPRHNANRPVSSVLGGTR"
     gene            4676..4939
                     /locus_tag="VN496_02870"
     CDS             4676..4939
                     /locus_tag="VN496_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15916.1"
                     /translation="MNIFVGNLSQEVTETDLVELFKQYGQVKSSEVKRDLFSGVTKGF
                     GFVEMPGKNHSLAAIAGLDGKDFKGQPLRVNEAREFPSRGRRR"
BASE COUNT         1042 a         1522 c         1594 g         1049 t
ORIGIN      
        1 ttcgtgactg cgctcgaaat tgcggcgctg gaccatatgc gcatggtggc ggccattgcg
       61 ccgttcgtgg attccgccat cagcaagacc gtgaatgtcc cggaaaacta tccgttcgag
      121 gatttcaggg atctgtattt cgaagcgtgg aaagcggggc tgaaaggcat cacgacctac
      181 cgcccgaacg gcgtactggg ctcggtcctg tccgttacgc cagcgagcga agcgcccaat
      241 gatctggata catcggacgc tgatcgccgc atccgcctgg atgccgcacc catgcctgcg
      301 ctgtcgagcc tgcgctggcc ggggcggccg cagatgacgg caggcaatcc gtcctggacg
      361 tatatggttg agtcgccatt tggggcgttc gcgattttcg tcggccacgt cgacaacggc
      421 gatccacatg ctttcgaagt ctgggtcaat ggcaacgaac agccgcgcgg tctgggcgcg
      481 gtggccaaga cgctgtcgat ggacatgcgt gcgcaggatc gtgcgtgggt gcgcaccaag
      541 ctcgacatcc tgtcgcgcac cgctggcgac gatgcgttcg actgtccgat gccgccggac
      601 ggcgcaccgg tgcgcatgcc gagtacggtc gctgcgtttg cgcgcatagt cagttggcgc
      661 gtcgaacaac tcggcggcct gacgccgcgt gatgaccgct cgccggtgct cgacgccttg
      721 ttcgcaaaga aagaacccaa gactggcact gacggcacca tgtcatggtc ggtggacgtg
      781 ttgaaccgca gttcaggcga cgatttcgtg ctgatcctga aggaactggt gttgccgtcg
      841 ggccagcgcc gtccttattc agtctggatg gcgggcagtt atccgcgctc gctggatggc
      901 ttgtgcaaat tgctatcgct cgacatgcgc gtaatcgatc cggcgtggat tggcatgaag
      961 ctgcgcaagc tgctgagcta tgccgaaccg ctgggcgatt tcatggcaaa agtgccgggg
     1021 gaagacaagc agcgcaattg gccgagcacg gtggcctacg tcgcgcaact catgattcat
     1081 cgctttgcga tgctgggcgt gctggacgag cgcggttatc cgatcgatga gatgggcgtg
     1141 ctggagatac cgcaagccgc gcgcgcacgc gcggcgcgca gcttgcagat tctgccggga
     1201 aagcgttgtc tcgaatgcgg aaacagcgcg gtgatccgaa aagacggctg cgaattctgc
     1261 acggcgtgcg gagcggtcgg cgcgtgcggc taaattaaaa acgggactga ggtgagtaaa
     1321 agcaggactg aggtatgagg actgagtgaa agacgaaaat agaaaagcat aaaaagaacg
     1381 agctcggagt acccttttag accggtatcc ccttgcggga cagtcctcag tcctcagtcc
     1441 tcagtcctaa gcgaagctcc gcggctgacc ggtggcttcg atcaccgaca tccacaagtc
     1501 agccgtgaca tcgacctgtt tgcggctgcg cgtggccagc ggcatgggca catgcacgaa
     1561 gcggccgtgc cagcgcccga tcagcatttc ggtgttgcct gccattgccg cgtgtactgc
     1621 attgcgcgcc atgttccaac agtagacgct gtccgagggc gtcgccggca cgctacggat
     1681 ggtgtagctc ggatcgatgt atttcagcgt tatctcgcgt ttggcctggc ggtagtgttc
     1741 ggttatgcgc tcgcgcagat acacgccgat atccttcagc ttcgcattgc cgcttttgtc
     1801 cgtcgcgccg tcggccgcat catctgtcat taattcctgg gcagcgcctt cagcgactac
     1861 gacgaccgcg tggcctttgc gcgcgagcac gcggtctaga ttggcgaaca cgccgcgttc
     1921 gccttccagc atggcgttga cttcggggat tagcaccaga tcgacatcgg cggacgcgag
     1981 cgcggcctgg caggcgacga agccggaatg gcggcccatc agtttgacaa ggccgatacc
     2041 attgcgcgcg cccatcgcct cgaccctcgc gctgcgaact acatccacgg cggcggagta
     2101 cgcactttcg aagccgaagc tgcgatcgat gaagtggata tcgttgtcga tggtcttcgg
     2161 tacgccgacc accgcgattt tgaggccgcg tcgttcaatt tcctccgtga aatgcattgc
     2221 gccgcgaatc gtgccatcgc ccccgatcac gaacaggatg tttatgccat tggcttcaag
     2281 attgtcgatg acttcgccgg ggtcttgtga cccccgcgag gaaccgagga tggtgccgcc
     2341 ctgatggtga atatgcgcca ccgactccgc cgtaagggtg acggggtcat ggccaaaccg
     2401 ggatatcagt ccctcgtagc cgaagcggaa tcccagaatc cgcttcacgc cgtagcctcg
     2461 cgcaagttcg agcaccaggc cgcgcacgac gttgttgatc cctgggcaaa gcccgccgca
     2521 ggtcacgatg ccacacccga ccgtgcgcgg atcgaagaac actttgtcgc gcggtcccgc
     2581 cagttcgaaa gccggcacgt tgccggcgcg cgcgacattg tcgccgaaga cggacatgcg
     2641 atcgtccacc aacacctttt ctgccgctcc ggcaaacatc acggcgcttt cgccaaggtg
     2701 cgcgactaca ggcgagggca gcaggcactt gccaagatcc ttgatgcgaa catcggccgc
     2761 ggtgattggc cccgccagcg cggtggaccc cgaaagggtg accgccgatg gtgcattgac
     2821 tgcattatgt ttggcgagag cgctcatata attttatgtg gcagttggtc gaaacggtgc
     2881 ggagcgcacc ggcgcggtgc ttcttgaacg gtgccgacct gcgtagaccc attgattcac
     2941 gggaggtttc aatccaccga taaatgtccg cgagcatcga cggtcaacgt tgcaaggaac
     3001 gtgcgagaaa tccgcgtccc tgaaaatcca ataaaaatcc aatataaaaa aagcgacttt
     3061 cgttacggaa accggtatga tgcgcgcaag gattttgttt cgcgtcaatc ggtccgcgtt
     3121 tcagcccgtt cgtggtagtt ctgcttcctc gaataatttc aactctgttc gattctgcct
     3181 gaaccggttc ggaatgccct ctgggcttcc gcaggctttg tcaggagata tccattagca
     3241 ctacatttga agcgctcggc cttcgtgccg agctggtccg agctgttgcc gacgaaggtt
     3301 atacagaacc caccccagta caggagcaag ccatcccggt gattctcgca ggccgcgata
     3361 ttctcgccgg cgcgcaaacc ggtaccggga agactgccgg atttgcgctg ccgctgctgc
     3421 agttgctggc ggcgcgtcat gcgggaggtg atgctcatcg ccgtccgatt cgcgcgctga
     3481 tcctcacgcc gacgcgcgaa ctcgcggcgc agatcgaaga cagcgtccgc acctatgggc
     3541 gccacctgcg catgaaatcg accacggtct ttggcggcgt cagcatgaat caacagatcg
     3601 gcgcgctgcg ccggggtgtg gatatcctgg tcgccacgcc gggacgcctg ctggaccatc
     3661 tgcaaagccg cacggttgac ttgtcgcgcg tggaaatcct ggtgctggac gaggcggatc
     3721 gcatgctgga catgggattc gtgcgcgaca ttcgccgcat cctgatgacc ttgcccgcta
     3781 aacggcagaa tctgtttttc tccgcaacct tctccagcga aatacgtgaa ctggccgact
     3841 ctttcctgca cgctccggtg caagtggcgg tcgcacaaag caccgttacg gccgacctga
     3901 tttcacaggt ggttcatccg gtggacaagg atcgcaagcg tgcaatgctc gagcatctgg
     3961 ttaataccgg cgactggcgg caggtactcg tgttcgcgcg taccaagcat ggcgcgaacc
     4021 gacttgccga acagttacag aaaaacggca ttagtgccga cgccatccac ggcaataaga
     4081 gccagggtca gcgcacccgt gcgctggccc agttcaagca gggcaaagtg cgcgtgctcg
     4141 tggcgactga cattgctgcg cgcggcctgg acattgaaca gttgccgcac gtagtgaact
     4201 atgaattgcc gaatgtgccc gaagattata tccatcgtat cggccgtacc ggtcgtgcgg
     4261 gcagttccgg ccaggcagta tcgctggtgt gctcggaaga gcgtgatttt ctgcgcgaca
     4321 tcgaacgcct gctcaaacgc agcatcgagc gtgtcgtggt cgaaggattc cagccgaccg
     4381 gcgcgccgct cagaactgat agccattccc cggcatcccg tgaacgccgt ccacagcagc
     4441 gaagccaatc ggacaggcca cgtacgcagg ggcgtgacgt acgcactggc agcggcgaag
     4501 gacgtcgcag ccagggtaat gctggaagac ccgcacgcaa cggcggcgag ggccgtcagc
     4561 gcagcggcgc cagcgcccgt cgcggcgact cgcagccgcg ccacaacgca aatcgcccgg
     4621 tatcttcggt gctgggtggc acccgctgat cgattatttt tgaaaggagg agcccatgaa
     4681 tatatttgtc ggcaacttgt cgcaggaagt cactgaaacc gacctggtgg agctgttcaa
     4741 gcaatatggc caggtaaaat cctcagaggt caagcgcgac ctgttttctg gtgtgaccaa
     4801 aggcttcggc tttgtcgaaa tgcccggtaa gaatcactcg ctggctgcca ttgccggcct
     4861 ggacggaaaa gacttcaagg gtcaaccgct gcgcgtgaac gaggcgcgcg agtttccgtc
     4921 gaggggacgg cgcagataaa ggcaggacca aggaccgaaa attccaggac tgaggtacga
     4981 ggactgagga ctgagcaaga gctaaaaggg ccgcattgcg gtccttttgt ttgtttagtt
     5041 ctttggtttg attactcagt cctttgactt ttacttagtc ctcagtcctc gtacctcagt
     5101 cctgtctttc actcagtcct catctctcag tcctgctttt gccgagcttg ttttctaaca
     5161 agctcggcaa aagcgcccgc cggtagcggc acgctgaagt agtagcc
//
LOCUS       DATMZQ010000095         5320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_425553, whole genome shotgun sequence.
ACCESSION   DATMZQ010000095 DATMZQ010000000
VERSION     DATMZQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425553"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VN496_02875"
     CDS             <1..500
                     /locus_tag="VN496_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15917.1"
                     /translation="PNNILSLGGGISYGSMRLTKSGKDLILATGGTDQITFKNWYAQG
                     NRSVAALQVFTEAMPGYNPAGTNPLLDNKVEQFNFTEIAKAFDTAGQANGWALTNALL
                     ATHLSGSDTAAFGGDLAYQYGLNGSLAGISLSQAQQVLNAPQFGTGVQTLRPVAELQQ
                     GRRLS"
     gene            528..1895
                     /locus_tag="VN496_02880"
     CDS             528..1895
                     /locus_tag="VN496_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXF15918.1"
                     /translation="MSKTTRPIDAGALDFAPAILRAQHENAPPLSRIVLYIALALFAV
                     MLLWAFFGRLDIIAVAQGKLIPGTYVKIVQPADSGVVREILIKDGQEVKAGQVLMRMD
                     TQVSDADSATVDNDLHLRQLQLRRIQAEMNGAAFTPAKGDPPAMFRQVDSQFRANREA
                     YRNQIENERATLAKAEQDLKGAIEMESRLKQTVPIYRETEAAHQNLAKDGFISKLAML
                     DKTRERIEKEQELKAQSYQVASLKASIDQSHKRLAQITSTYRQQLQNERSDADTQRLR
                     LEQEASKQAYKHALLELKAPHDGVVKDLATHTSGTVVSPGTILMTLVPHDDPVKAEVW
                     VTNEDAGWVELDQSVKLKLAAFPFNKYGMVKGRVEYVSPDAAELPDTRERDRKDTREH
                     VMPPSGFRTLVTLESPYIEAAGKRYRLSAGMTVTAEVNLGSRTVMEYLLSPVQKVAHE
                     AGREM"
     gene            complement(2026..2349)
                     /locus_tag="VN496_02885"
     CDS             complement(2026..2349)
                     /locus_tag="VN496_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15919.1"
                     /translation="MKKLDRYERELLGAYEKGVLVSVRPTKAQLGGFREAARATFVKS
                     KRVNIRLSPLISWTFRPRAYEEGVPYQTLIASVLHKYVSGRLHEKKSRLTAGSTATGR
                     KRAAR"
     gene            2542..3975
                     /locus_tag="VN496_02890"
     CDS             2542..3975
                     /locus_tag="VN496_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXF15920.1"
                     /translation="MPKPVASSLDAKSVEQFVSMKWDEDVVPRLCEYIRIPCKSPHFD
                     AGWATNGHLDAAVELAEKWARAQPVRGLKIEAVRLVGRTPVILFELAGELPGAILMYG
                     HLDKQPEMTGWKESYGPWTPVLHEGKLYGRGGADDGYAIFACLVALAALKEQGVPHAR
                     IVGLIECCEESGSYDLPAYLEALKSRIGTVDLVIGLDSGCGNYEQLWMTTSLRGIAAG
                     TLSVEVLSEGVHSGDASGVVPSSFRIARQLLDRLEDSRTGRILPKEFYCDIPAERIEQ
                     AALAGKTLGDAMLTRFPFAGKTQPMTRDHGEAVLNRTWRPFLSVIGADFMPAIRDAGN
                     VLRPKTALKLSLRIPPLIDGQRATMDLKRILEADPPHGAKVSFEPDQGATGWHAPATA
                     PWLKSAVDAVSKQYYGKPAMMMGEGGTIPFMAMLGKSFPKAQFLITGVLGPKSNAHGP
                     NEFLHVPYAKKLTACVAGVIARYGTSA"
     gene            4066..>5320
                     /locus_tag="VN496_02895"
     CDS             4066..>5320
                     /locus_tag="VN496_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HXF15921.1"
                     /translation="MSDSKKAPSKPRVLDARPDRLDFRDLPYRAPLRSLPLCFPLEKD
                     VGRYIADYVAEGLVLNQGNQGACTGFGLACVANYLFWTRHVETRTRRKFVSVSPRMLY
                     ELAKRYDEWPGIDYEGSSCRGALKGWNKHGICSADEWPYKLDRHGNPIFVRPDPNWQL
                     DASQRPLGVYYRIDRESIVDLQSAISEIGAIFVSAGAHDGWDALAHTRRKSAPPKKHK
                     DLPTIPPVEDRKSIGGHAFALVGYNERGFVVQNSWGESWGASGFAVLPYDDWVVNATD
                     AWACALGVPVSSSDTSGGKLVPTIPTRWRVPPGRSLTRLDRNTREPANPADDPWPIDH
                     PFNFKDYQPWSTANAYKHTLVTGNNGELAATDFTRDVGDKQGLAEEIIRTGPQEWSGP
                     KTKTLKLIIYAHGGLNAESESVQRIR"
BASE COUNT         1195 a         1526 c         1572 g         1027 t
ORIGIN      
        1 gtccgaacaa catcctctca ctgggcggcg gtattagcta cgggagcatg aggcttacga
       61 aatccgggaa ggacctcatc cttgcaaccg gcggtaccga tcaaatcaca ttcaagaact
      121 ggtatgcgca aggaaatcgc agtgtcgcgg cgctacaggt ctttaccgaa gcaatgccgg
      181 gctacaaccc cgccggcacc aacccgctac tcgataataa agtcgaacag ttcaacttta
      241 ccgagatcgc caaggcgttc gataccgcgg gacaggcaaa tggctgggca ctgacgaacg
      301 cactgctcgc tacgcatctt tcgggcagcg atacagcggc cttcggcgga gatctcgctt
      361 accagtacgg attgaatggc agcctggccg ggatcagcct ttcgcaggcg cagcaggtac
      421 tgaacgcgcc gcagttcgga accggagtgc agacactgcg gccagtcgcc gaattgcagc
      481 aggggcggag gctgtcctga cgagatggaa agggggataa catcatcttg agcaagacca
      541 cgagaccaat cgatgcgggc gcactcgatt tcgcaccggc gatcttgcgc gcccagcatg
      601 aaaacgcacc tccgctgtcg aggatagtcc tttacatcgc actcgcgctg tttgcagtga
      661 tgctgctctg ggccttcttt gggcggctgg acatcatcgc ggttgcgcag gggaaactca
      721 ttccaggcac ctatgtgaag atcgtccagc cggctgatag cggagtcgtt agggagatcc
      781 tgatcaaaga cggacaggaa gtgaaggccg gccaggtgtt gatgcgcatg gatactcagg
      841 tttccgatgc cgattccgcg acggtggaca atgatcttca cctgcggcag ttgcaattgc
      901 ggcgcatcca ggcggaaatg aatggtgcgg ctttcacacc ggccaaaggc gacccacccg
      961 cgatgttcag gcaagtcgat tcgcaattcc gcgccaaccg cgaagcctac cggaaccaga
     1021 tcgagaacga acgcgctacg cttgcaaagg ccgagcagga tctgaaaggt gcgatcgaga
     1081 tggagtcccg gctcaagcag accgtgccga tctaccgcga aacggaagcc gctcaccaga
     1141 acctcgccaa ggatggtttt atttcaaagc ttgccatgct ggataagact cgggaacgta
     1201 tcgagaagga acaggaactc aaggcgcagt cctaccaggt tgccagcctc aaggccagca
     1261 tcgatcagtc gcataaacgc ctggcgcaaa ttacctcgac ctatcggcag cagttgcaga
     1321 atgagcgcag cgatgccgac acgcaacggc tgcgtctgga acaagaagcg agcaagcagg
     1381 cctacaagca tgcgttgctc gaacttaaag cgccgcacga tggagtagtg aaagaccttg
     1441 ccacgcacac gtcgggaacc gtggtttcac cgggaacgat tctgatgacc ttggtcccac
     1501 atgacgatcc agtgaaagcg gaagtctggg tcaccaacga agatgccggc tgggtggagc
     1561 tcgatcaatc cgtgaagctg aagttagccg cgtttccgtt caacaagtac ggcatggtga
     1621 aaggccgcgt cgaatacgtc agcccggacg ctgccgagtt gcccgacacg cgtgagcgcg
     1681 accgcaaaga tacccgcgag catgtcatgc cgccatccgg attccgcacg cttgttacgc
     1741 tcgaatcgcc gtacatcgaa gccgcaggca aacgctatcg cctgagtgca ggcatgaccg
     1801 ttaccgcgga agttaacctc ggcagccgca cggtgatgga atatctgctc tcgccggtgc
     1861 agaaagtcgc gcacgaggcg gggcgggaga tgtagaagtt agattcaagg attctcgcgc
     1921 aagctggttc atccttgaac cgctcaagca ttgcaaacta tcccgagagc taacttctcc
     1981 ggcacgaaaa tatctctttc gttcatatgg agctaacgtc gcgggctaac gcgccgcccg
     2041 ctttcggccg gtcgctgttg agccggcggt tagacgtgac ttcttctcat gcaggcgccc
     2101 cgacacgtat ttatgaagca cgcttgcgat tagcgtctgg tagggcaccc cctcttcgta
     2161 cgccctgggc ctgaatgtcc atgagatcag cggcgatagg cgaatattta cgcgtttgct
     2221 cttaacgaaa gttgcacgtg cggcctcccg aaacccgccg agctgtgcct tggtcgggcg
     2281 cacggacacc aatacccctt tttcgtaggc gcccaataat tcgcgctcgt aacggtcaag
     2341 ttttttcatc tggatcatcc actcctaaat aatctggcct tgacgggcag taatctaact
     2401 cgaatcgctg cttgaaactg aatttccgtt tgtcggataa ggcatcgcca ttctcactta
     2461 gtcttgagcg acgcgggcca ttggctctcg ctgtgttgcc tctacaatag acatctcacc
     2521 cttacacgca aggggatctc catgccgaaa ccagtcgcca gttcccttga tgcaaagtcc
     2581 gtcgagcagt tcgtctcgat gaagtgggac gaagatgttg ttccgcggct atgcgaatac
     2641 attcgtatcc cgtgcaagtc gccgcacttc gatgccggct gggcaacaaa cggccacctc
     2701 gatgctgcgg tcgagctcgc agagaagtgg gcacgtgcgc aacctgtccg cgggcttaag
     2761 atcgaggccg tgcgcctggt aggccgcacg ccggtgattt tgtttgagct cgcgggcgaa
     2821 ctgcccggcg ccatcctgat gtacggtcat ctcgacaagc agccggagat gactggctgg
     2881 aaagaaagct acgggccgtg gaccccggtg ctgcacgaag gcaaacttta cggccgtggc
     2941 ggtgccgatg acggctatgc gatatttgca tgcctggtgg cgctggccgc gctgaaagaa
     3001 cagggcgtgc cacatgcgcg catcgttggg ctgattgaat gctgcgaaga aagcggcagc
     3061 tacgacttgc cagcttatct cgaggcgctc aagtcccgca tcggcacggt ggaccttgtc
     3121 atcgggctcg attccggttg cggcaattac gagcagttgt ggatgaccac cagcctgcgc
     3181 ggcatcgccg ccggcacgtt gagcgtcgag gtgctcagcg aaggcgtgca ctccggcgat
     3241 gccagcggtg tggtgccatc cagctttcgc atcgcacggc aactgctcga tcgcctggaa
     3301 gactccagga ccggccgcat cctgccgaaa gagttttatt gcgacatccc cgccgagcgt
     3361 atcgagcagg cggcgcttgc cggcaagacg ctcggcgatg cgatgctgac gcgcttcccg
     3421 ttcgcaggta aaacgcagcc catgacgcgc gaccatggcg aagcggtgtt gaaccgcacg
     3481 tggcggccat tcctttccgt gatcggcgcc gatttcatgc cggcgatcag ggacgcaggc
     3541 aatgtgctgc gcccgaagac cgcgctcaaa ttgagcctgc gtatcccgcc cctcatcgat
     3601 ggccaacgcg cgaccatgga cctgaagcgg attctcgaag ccgatccgcc gcacggcgcg
     3661 aaagtgagct ttgagcccga tcagggcgcg actggctggc acgcgccggc aacggcgccg
     3721 tggttaaagt ccgcggtcga tgccgtctcg aaacagtact acggcaaacc ggccatgatg
     3781 atgggcgagg gtggaaccat tccgttcatg gccatgctcg gcaaatcttt cccgaaggcc
     3841 cagttcctga ttaccggcgt gctcgggccg aagtccaacg cgcacgggcc gaacgagttt
     3901 ctgcacgtgc cttatgcgaa gaaattgacg gcctgcgtag caggcgtgat cgcacgctac
     3961 ggaacatcag cctaaaagat tctcggtacg gaaatcggca aaaggcgact tgccaacaaa
     4021 agttgaagaa cccggaggtt tggcgtggca acgacaaaaa aagcgatgtc tgattcaaaa
     4081 aaagcaccgt caaaacctcg tgtactggat gcgcggccgg accggcttga ttttcgcgat
     4141 ttgccctacc gtgcaccatt acggtcgttg cccctttgct ttccacttga aaaggatgtc
     4201 ggcaggtata tcgccgacta cgtcgccgaa ggcttggtgc tgaaccaggg aaaccagggt
     4261 gcgtgcaccg gattcggttt ggcttgcgtg gcgaattacc tgttctggac gcggcatgtg
     4321 gaaacccgta ctaggcgaaa attcgtatcg gtcagcccgc gtatgctcta tgagctggcc
     4381 aagcgatacg acgagtggcc cgggatagac tacgagggat cgagctgccg tggcgcactg
     4441 aaaggctgga acaaacacgg catatgcagt gccgacgagt ggccctataa gctcgatcgg
     4501 cacggcaacc caatcttcgt tcggccagac ccgaactggc agctggatgc cagccagaga
     4561 ccgttggggg tttactaccg gattgatcgt gaatcgattg tggatttgca atcggctatc
     4621 agcgagattg gcgcgatatt cgtttcggcc ggagcgcacg atggatggga tgcccttgca
     4681 catactcgcc gcaagagtgc gccacccaaa aagcacaagg acctgccgac aattccgccg
     4741 gtagaagaca ggaagagcat tggcggccat gctttcgcat tggtcggcta caacgagcgg
     4801 gggtttgtag ttcaaaactc ctggggagaa agctggggcg cttcaggatt cgcagtactg
     4861 ccttacgacg attgggtggt caacgccacc gacgcgtggg cttgtgcgct tggtgttccg
     4921 gtttcatcga gcgacacatc gggcggaaaa ctggtcccaa cgatcccgac ccgctggcga
     4981 gttcctcccg gcagatcgct caccaggctc gatcggaaca cacgggagcc agccaaccct
     5041 gcagatgatc cctggccgat cgaccatcca ttcaatttca aggattatca gccgtggtcc
     5101 actgcaaacg catacaagca tacgctcgtc accggcaaca acggcgaatt ggcggctaca
     5161 gatttcacga gggatgtcgg cgacaagcag gggcttgccg aggaaatcat acgcaccggt
     5221 ccccaggaat ggtcagggcc aaaaactaaa acgctcaagc tgatcatcta cgctcacggc
     5281 ggcttgaatg ctgaatcaga atcggtgcag cgaattcgtg
//
LOCUS       DATMZQ010000096         9949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3187283, whole genome shotgun sequence.
ACCESSION   DATMZQ010000096 DATMZQ010000000
VERSION     DATMZQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3187283"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..232)
                     /locus_tag="VN496_02900"
     CDS             complement(83..232)
                     /locus_tag="VN496_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15922.1"
                     /translation="MKSKLEKTVGVYERPQRPKAPRLVLGVVIALVAVAVIVVWIVRS
                     GVLIS"
     gene            complement(282..1304)
                     /locus_tag="VN496_02905"
     CDS             complement(282..1304)
                     /locus_tag="VN496_02905"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HXF15923.1"
                     /translation="MAKTVVALMETPQEAEDVVRDLTSTCGCERSDIGLMARGSQEDA
                     SAMTGGETSGEGHSTVASGALKGAGTGAAVGGILGLVAGAAALTIPGIGPFVAAGPIA
                     SALAGAGIGAAAGGAIGALVHLGVPEEEAHYYAEGVRRGGTLITVNASTDEMATCAAM
                     VMKNHGAADIEQRAAEWKQQGWGGRLTADNEQQEEEVLPVTQEELVVGKREIGQGVVR
                     VYSQVSETPVQENVQLREEHVQVERRPVDRPVEAGDEVFQEKSIEMREMAEEPVVSKR
                     SRVIEEVRVGKQVKQRDQTVADTVRKTDVRVEQTGQGQSTSGYQPERRMNPSSSYSGD
                     DRRMAH"
     gene            complement(1333..1770)
                     /locus_tag="VN496_02910"
     CDS             complement(1333..1770)
                     /locus_tag="VN496_02910"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HXF15924.1"
                     /translation="MSPVHRSSIDESNDAAVTIPLGREEVELGKCAVQQGVVRIQTTV
                     REEERIVETRLLKDEITVERVEMNRPIDGPIEARYEGDVLVIPVIEEVLVVRKQLVLK
                     EELRITQRSVARPHSQAVKLRHEEATVEREATGEQSAKDAPST"
     gene            2127..2321
                     /locus_tag="VN496_02915"
     CDS             2127..2321
                     /locus_tag="VN496_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HXF15925.1"
                     /translation="MEDDLSRRGSSDSGRVSLSEDWERRYWARTFEVTEQELQEAVAA
                     VGPYVDALRKHFRFPPKVAR"
     gene            2482..3096
                     /locus_tag="VN496_02920"
     CDS             2482..3096
                     /locus_tag="VN496_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15926.1"
                     /translation="MRYQTYIAAVILGISPVAAFAAEADQQQPYATPQASDSEQNSSN
                     TTTGDQQSDTYSAGEEGKHKTGQASSANKSEARSNQNAATQSGTNGASPEAKRKTGQS
                     SSAQESKASSGQSEQSMSEPSDSDQESSASDQDEDSGGTSDTPKRLVIVVPEEMQDTM
                     EQLIAFLKASPDAEILIMAPGESAADENTFRLQSGGSSEEDEDQ"
     gene            complement(3211..4719)
                     /locus_tag="VN496_02925"
     CDS             complement(3211..4719)
                     /locus_tag="VN496_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXF15927.1"
                     /translation="MELLNHLALGFSVALTPVNLLYALTGALLGTLIGVLPGIGPVAT
                     IAMLLPTTFTLEPVSALIMLAGIYYGAQYGGSTTSILLNMPGETSSAVTCLDGYQMAR
                     QGNAGAALSVAALGSFFAGCVATIMIATVAVPLSEFALNFGPAEYCSLMVLGLVGSVV
                     LAHGSLLKAFAMVILGLLLGIVGTDVGTGTARFVFGIPELADGIGVVSVAMGLFGFAE
                     IISNLESTEKREILTSKLGGMWPTRAQFRQSWPAVLRGTGLGSVLGLFPGGGAMLASF
                     ASYALEKKVADDPTRFGKGAIEGVAGPESANNAGAQSSFIPLLTLGIPENAVMALMVG
                     AMTIHSIQPGPQVMTTNPALFWGLIASMWVGNLMLVVLNLPLIGMWIKLLHVPYRVLY
                     PAILLFCAIGVYTTSNATFDVMQTAFFGLIGVLFVKLACEPAPLLLGFVLGPMLEENL
                     RRAMLLARGNALVFVKRPLSLAMLSLAVILLLLIILPHFRKTREQAFRETDS"
     gene            complement(4729..5181)
                     /locus_tag="VN496_02930"
     CDS             complement(4729..5181)
                     /locus_tag="VN496_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXF15928.1"
                     /translation="MPTIRHPKDFFAGLLFAAVGAGAIVISTRYTLGTASHMGPGYFP
                     RMLGLLLGVLGVVLSLRALRLRGEPFAAWKWRPTIVVLGSVVLFGAIVTPLGLVLSTI
                     ILIVLSSAASAEFRPREAVVASVLLAAVVVGVFVYGLHLQLPIWPGTK"
     gene            5482..5664
                     /locus_tag="VN496_02935"
     CDS             5482..5664
                     /locus_tag="VN496_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXF15929.1"
                     /translation="MKKEQVTGRAEQAKGTVKEAAGKATGDKTLRVKGKAQKAAGAVK
                     SAYGDAKDKAEKDHNA"
     gene            5657..6016
                     /locus_tag="VN496_02940"
     CDS             5657..6016
                     /locus_tag="VN496_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15930.1"
                     /translation="MPRIISRTGDQRSDNLSLPLSDRTQRRFVIFAQPKEYGDHPNSY
                     YSRTGQQTTVRAQAAQFASEMEAENFALSMGIKLTIMTYVGEERFAQSELENPPKTNR
                     SFRRRKVDSRDSLARRD"
     gene            6283..6504
                     /locus_tag="VN496_02945"
     CDS             6283..6504
                     /locus_tag="VN496_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15931.1"
                     /translation="MSSKVWQFVEVPGEGWCWQRGDTDDPVTSKNSFPDLASCIFNAE
                     ENGFRPAKPERRRRPRNQPGCEMRCVGGA"
     gene            complement(6968..7159)
                     /locus_tag="VN496_02950"
     CDS             complement(6968..7159)
                     /locus_tag="VN496_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15932.1"
                     /translation="MMFENGVHSRPGCAIYRTVPDDADGYLYYFSPAAAEQYKEFLQF
                     WGSVDIPEPDHLGWMRRIA"
     gene            complement(7357..8760)
                     /locus_tag="VN496_02955"
     CDS             complement(7357..8760)
                     /locus_tag="VN496_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HXF15933.1"
                     /translation="MPSRKMFVLVPTPLEPLDAGKSMSKSRKKNDPLDVGKRSALFST
                     AAVLTDHGLRPGMDASRCDAFDNNIKKNGLRYVAMLLLSLLLTACGQGSGNNANTTSS
                     AANAPAPPLVVKSPSPPPDSSPPTPTPPPVQTGTARYFVYVANFGDGTVSAYTALTSG
                     HLQSNGGVNVGAGAVSIAIMPSGKFAYVANANGDSLSAFMVDSATGALTAIGGSPFAT
                     GTNPFHVMVHPSGQFVYVANRASNDVSAFSIDPDSGVLTRLAGSPFTAGGSPFAIAVE
                     PAGRFAYVANFGSNDISAFAVSAAGALKEVLGSPFAAGTAPASIAIDPAGHFAYVADY
                     DSNDISVFSINASGALALTATVPIENGLHPVCITLNSDGTLAYVANRDTGNISAFTIA
                     DSGTLVAVPGSPFVAGRGPFAVTLDPAGTFAYTPNQNSDSVSGFTIDASTGTLALLGS
                     VPTGKEPVAVAITKGAL"
     gene            complement(8911..9084)
                     /locus_tag="VN496_02960"
     CDS             complement(8911..9084)
                     /locus_tag="VN496_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HXF15934.1"
                     /translation="MLKWAAIFFIVSVIAGFFGFTDVAAGTRGVAKILFFIALAFFLV
                     VLIFGVFLGVLVF"
     gene            9299..9532
                     /locus_tag="VN496_02965"
     CDS             9299..9532
                     /locus_tag="VN496_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15935.1"
                     /translation="MVPLTCVVAVGLPQRIPPEEEELIREGYVSVRGDVQLEEYRRRA
                     SDQVGVSITLMWSDDGRPLYARESIPPVDLLND"
     gene            complement(9770..>9949)
                     /locus_tag="VN496_02970"
     CDS             complement(9770..>9949)
                     /locus_tag="VN496_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15936.1"
                     /translation="CWPVDPPRVLVLSGAFAALLFVSVVAQPPVITPMLKSKSPRATT
                     DRIFILLAFKEALTG"
BASE COUNT         2232 a         2825 c         2891 g         2001 t
ORIGIN      
        1 gcgcggcgac acgcaattta tttcctggaa ccaatgctcg ccgatcaacg cactactggc
       61 gagggcttgg ctcggactgc tgctaactga tcaggactcc cgaccggact atccacacca
      121 ctatcactgc cacggccaca agggcaatca ccacgcccaa taccaagcgc ggtgccttcg
      181 ggcgctgcgg ccgttcatac acgccgacgg ttttttcaag cttcgatttc atgcagtgca
      241 gggtgcggca taaggaatgc cgcacccgct gacggtttgc atcaatgcgc catgcgacga
      301 tcgtctccgc tgtacgaaga cgatggattc atgcgccgct caggctggta gcctgatgtg
      361 gattggccct ggccggtttg ttccactcgc acgtccgtct tgcggaccgt gtcggctacg
      421 gtctgatcgc gctgttttac ctgcttgcca acgcgaactt cttcgattac ccggctgcgt
      481 ttgctgacca caggctcttc ggccatttcc cgcatctcga tggacttttc ctggaacacc
      541 tcgtccccgg cttccaccgg acgatcgacc gggcgccgct cgacctgcac atgctcctcg
      601 cgtagttgta cgttctcctg gaccggagtt tcgctgacct gcgagtagac gcgcaccacg
      661 ccctggccaa tctcgcgttt gcccacgacg agttcttctt gcgtcactgg caatacttct
      721 tcttcttgtt gttcgttgtc cgctgtcagc cggcctcccc agccttgctg cttccattca
      781 gctgcgcgct gctcaatatc tgcggcccca tggtttttca tgaccatagc tgcacaagtc
      841 gccatctcat cggtgctggc gttgacggtg atcaacgtgc cgccgcggcg cacgccttcc
      901 gcgtagtagt gtgcttcttc ctccggcacg ccaagatgca ccagtgcacc gattgcaccg
      961 ccggctgcgg caccgatgcc tgcaccggcg agtgcagacg cgattggtcc cgccgcgacg
     1021 aacggtccga tgcctggaat cgtcaatgcg gccgcgcctg cgaccaggcc aaggatccca
     1081 cccactgcgg ctccggtccc agcgcctttg agtgctccgg acgcgaccgt gctgtggcct
     1141 tcgccggacg tttcgccgcc ggtcatggca ctggcgtctt cctgggaacc gcgcgccatc
     1201 agtccaatgt cggatctttc acagccacag gtgctggtca gatctcgcac gacgtcttca
     1261 gcttcctgcg gggtttccat caaagcaacg actgtctttg ccatcgtatt ctccttgggg
     1321 ttgtgttgaa aatcatgtcg aaggggcgtc cttggcggac tgctcgccgg tcgcttcccg
     1381 ttcaaccgta gcttcttcgt gacgcagctt gaccgcctgc gaatgaggcc gtgcgacgga
     1441 gcgctgcgta atgcgcagtt cttccttcag caccagctgt ttgcgcacga ccaggacttc
     1501 ctcgatgacc gggatgacga gaacgtcgcc ttcgtatctg gcctcgatcg ggccgtcgat
     1561 cggccgattc atttcaacgc gttcgaccgt aatctcatcc ttcagaagtc tcgtttcgac
     1621 aatccgctct tcctcgcgca ccgtggtctg gatacgcacg acaccttgct ggactgcgca
     1681 cttgcccagt tccacttctt cgcggccgag cgggatggtt acggcagcgt cgttgctttc
     1741 gtcgatcgac gagcgatgaa ccggactcat tggcgcgtgt ccttgccgga atgcgggttg
     1801 aaatgtccct tcggccgctg ttcgcaaggg atgtgccaat tggcttacgc tcacgcggac
     1861 gtccgcgcgg acgcggcggt gccactcgcg gatgtagctt ctacaatttt ccgtcttcgc
     1921 ttggggcggg taacgcatgc cgaccgacat gccggtgttc aatagggccg tcgattgcca
     1981 atccgggcag ggcaacccgg tgggccggaa ggcctatgac ccgcggttgc agaagtctgt
     2041 cattgaatgt gccaccgcac gaaccttcgt acccgtgttg aatctatttc tcacataggg
     2101 agcaatgcgc aggaggagat tcgaccatgg aagacgatct ttccaggcgt ggttcatccg
     2161 acagcggcag ggtaagcctc agcgaagact gggaacgccg gtattgggcg agaacgtttg
     2221 aagtcacgga acaggaattg caagaggcag ttgcggcggt gggtccgtac gttgacgcgt
     2281 tacgcaagca tttcaggttc ccgccgaaag tagccaggta atgtaattaa taggtagcca
     2341 gctcatgaat cgcgcagttt gattttgcaa tcatcaacga caaatgcgcc acagcaatcg
     2401 atgcgaactg gcgtcgattt gcaaagatct tcaggacgcg gaacttccgg ggccatgcaa
     2461 ctcacaatca ggagattcgc aatgagatat caaacctata ttgccgcggt aattctgggt
     2521 atttcgccag tcgcagcctt cgctgccgag gcggatcagc aacaaccgta cgccacgcca
     2581 caagcgtcag acagtgaaca gaattcttcg aacacgacga ccggcgacca gcaatccgat
     2641 acgtattccg caggcgagga aggcaagcac aagacgggac aagcctccag cgcgaacaag
     2701 agcgaagcgc gctcgaacca gaacgcagcc acgcaatccg gtaccaatgg cgccagtccg
     2761 gaagctaaac gcaagactgg ccaatcctcc agtgcgcaag agagcaaggc ttcatccggt
     2821 caatctgaac agagcatgtc cgagccgagc gattcggatc aagagtccag cgcaagcgat
     2881 caggatgagg attcgggcgg tacgtcagac acgcccaaga ggctggttat cgtggtgccg
     2941 gaagagatgc aggatacgat ggagcaactc attgctttcc tgaaggcttc tccggacgcc
     3001 gagatcctca tcatggcacc tggcgagtcc gctgccgacg agaatacctt taggctgcaa
     3061 tcgggcggct cttcggaaga ggatgaagac cagtaggctg catcgcgaat accagaatga
     3121 ggcgcgccgc tgacagggtg gcgcgcactt gtcgcagtgg gaagagcagt cccgcctaga
     3181 atcagcagac gggatacctg tgaagatagc ttagctgtcg gtttcgcgaa atgcctgctc
     3241 acgcgtctta cggaagtgcg gcaggataat cagcagcaac aatattacgg cgagcgacag
     3301 catggccagc gacagcgggc gctttacgaa gaccagcgca ttcccgcgag ccagcaacat
     3361 ggcgcggcgc agattttcct ccagcatcgg accgaggacg aaaccgagca aaagtggcgc
     3421 cggctcgcac gcgagcttga cgaacagcac accgatcagc ccgaagaacg cagtttgcat
     3481 gacgtcgaag gttgcattgc tggtggtgta aaccccgatc gcgcagaaca gcagaatagc
     3541 gggatagagc acccggtagg ggacgtgcag aagcttgatc cacatcccga tcagcggcag
     3601 attcagcacg accagcatca gattgccgac ccacatcgag gcgatcaatc cccagaacaa
     3661 cgcaggattg gtcgtcatga cctgcgggcc cggttggatg ctgtggatag tcatggcgcc
     3721 caccatcaaa gccataactg cgttttcagg aatgccgagc gtcagtagcg gaatgaaaga
     3781 cgattgcgcc ccggcattat tcgctgactc cgggcccgcg accccttcga tagctccctt
     3841 gccgaaccgg gttgggtcgt cggccacttt tttttccagt gcgtacgaag caaaagacgc
     3901 cagcatcgcg ccgccaccgg gaaatagtcc gagcacggat ccgagtccag tgccgcgcag
     3961 cactgctggc caggactggc gaaattgggc acgagtgggc cacatgcccc cgagtttgct
     4021 ggtcagaatt tcgcgcttct ccgtagactc gaggttgctg ataatttccg cgaagccgaa
     4081 caggcccatt gctacgctga caacgccgat gccatccgcc aattccggga tgccaaaaac
     4141 gaaacgcgcg gtgccagtgc cgacatctgt tcccacgatt ccaagcagaa gcccgaggat
     4201 aaccatggcg aaagccttca gcagcgagcc gtgggcaagc accacggagc ccaccagtcc
     4261 gagcaccatc agggagcaat actctgccgg cccaaaattg agtgcgaatt cggacagcgg
     4321 gaccgccacg gtggcgatca tgatggtcgc gacgcagccg gcgaaaaaag atcccaatgc
     4381 cgcaacagac aatgcggcac cggcgttacc ttgccgggcc atctgataac catcgaggca
     4441 agtgactgcc gaggatgttt cgcccggcat gttgagcagg atcgaggtgg tggaaccgcc
     4501 atactgcgca ccgtagtaga ttcccgccag catgatcagc gcggaaaccg gctccagcgt
     4561 gaacgtggtt ggaagcaaca tcgcgatggt cgcgaccgga ccaatgccgg gtaatacgcc
     4621 aatcaatgtt ccgagcagcg cgccggtcag tgcataaaga agattgaccg gcgtgagcgc
     4681 gacactaaac ccaagagcca ggtggttgag cagttccatc gagagtgtct atttcgttcc
     4741 gggccaaata ggcagctgaa ggtgcagccc gtacacaaac acgccgacga cgacagcggc
     4801 tagcagaacg ctggcgacca ctgcttccct gggacggaac tcagcacttg cggcgctgga
     4861 caagacgatc agaatgatag tggaaagtac tagtcccaaa ggcgtgacga tcgcgccaaa
     4921 cagcacgaca ctgcctaata caacgatcgt agggcgccac ttccatgcag caaacggttc
     4981 gccgcgtagc cgcaaagcgc gcaggcttaa cacaacgccc aggacaccca ggagaaggcc
     5041 cagcatgcgc ggaaagtagc ccggtcccat gtgggacgca gtcccaagtg tgtaccgagt
     5101 gctgattacg atcgcgcctg cacctacagc tgcgaacagc agccccgcaa agaaatcctt
     5161 ggggtgacgg attgttggca ttgttttcca ccctcgcagc tattgttgaa ccggccggac
     5221 catgacagcg ttttactgac accagcgctt aagcctagca ttgaaaaggt tccgacgtta
     5281 cttcgcggtt gctgtggccc gcgcacaaaa ctgcagttgg ctctgtacac atcgcaatga
     5341 tcggttccag tgcgcgtgca gcggccgcgt aacgttactg tgacaaagtg gtttctgtaa
     5401 gcgctgaact tttatcggca accgtaaatc tcatgggaag ccgacgtgca gtttgcatgt
     5461 cgatcctaca aggagaaggt catgaagaag gaacaagtga cgggccgtgc cgaacaagca
     5521 aaaggcacag tcaaggaagc cgccggcaag gcgaccggag acaagacgct gcgagtgaag
     5581 ggcaaggctc aaaaagcagc gggcgccgtc aagtcagctt acggcgacgc gaaggacaaa
     5641 gcagagaagg accataatgc ctaggattat ctcccgcacc ggcgatcagc gcagcgacaa
     5701 cttgtctttg ccgctcagtg acaggacgca acggcggttt gtgattttcg cgcaaccaaa
     5761 agagtatggc gatcacccaa acagttacta ttcccgtacg ggccagcaga cgacggtgcg
     5821 agctcaggcc gcccagttcg cttctgaaat ggaggcggag aattttgcgt tgtccatggg
     5881 catcaaactg acgatcatga cttatgtggg agaggaaaga ttcgcccagt cggaactcga
     5941 aaacccgccc aagacaaacc gctcctttcg aaggcgaaaa gtggatagca gggattccct
     6001 ggcgcgacgc gattagcgcg cggatgcggt tgcgtattca cccgatcggc ctcttggtcc
     6061 gtacagcaat tcagtttgcg attgctcttt cctgctcact gcctcgaacg tctttgtagg
     6121 caggactgcc agttgccgta gacattttcc tacgagaagt tatgcgtctc gaaatgttac
     6181 ggaagctgac aatttgcaaa ttatgttgcg ttttcgagcg ggcaccgtga ggcttgctat
     6241 gttgcttcag cgttcgccaa aatctgatac gggggaatgc gcatgagttc aaaagtctgg
     6301 caattcgtcg aagtcccggg ggaaggatgg tgctggcagc gcggtgatac agatgatccg
     6361 gtcaccagca aaaattcttt tccggatctc gccagctgta tctttaatgc cgaagagaat
     6421 ggctttcgcc ctgccaagcc ggagcggcgc aggcgtccca ggaaccagcc gggttgcgag
     6481 atgcgttgcg tcgggggagc gtagcttttg caaacggccc tccggctacc ggattgagga
     6541 atctttttca cccactgggc ttacacgatg tgcttgaggg ccatgggtgt tcgcggtgat
     6601 ggcgcaacgc gcattagcgc cctatcctgt gcctcaactt gaaaggtggc acatgcaacc
     6661 gatgcgatgg cagtttgagc gatctggcca ccagagttgg tactgggaaa agcagaagct
     6721 cggtgcagag cggatgtcgt tgagggtggg acccttcccg agctttacca gctgccttgt
     6781 cgatgcccag cagaagggct tcaaccctgg cagagccgaa agaagaaagc tgccccgcac
     6841 ggtgacgacc gctatgcccg aagcacggcg tcttctcagt gacgctgtca tgcgtatgag
     6901 acgtagctga cgcggcggtg tcacgaaagt tttttcctga tcgaacaaaa tcgcgacagg
     6961 cattgagtca cgcgatgcgc cgcatccagc ccaagtggtc gggttccgga atgtccacgc
     7021 taccccagaa ttgcagaaat tccttgtact gttccgcagc tgcgggagag aagtaataga
     7081 gatatccgtc tgcgtcatcg ggcacggtgc gataaatcgc gcagccggga cggctgtgca
     7141 cgccgttctc gaacatcata ttgagaaacg tccggttaac cgcatgaaag acgcaggtat
     7201 gcggaatatg aacacagttc caggctgcca attgctgcct cgtaaccgac cgtcaaacat
     7261 caacggtatg atttggtccg gatggaactg cctagttcca tcctttttgt gaatacttct
     7321 gaagagtcgc cagacgatgg gtgcgtatgg gaacgcttac agtgcgcctt tcgtgatggc
     7381 gacagccact ggttctttgc cggtcggcac gcttcccagc agcgccaggg tgccagtcga
     7441 tgcgtcgatt gtgaatccag acacggagtc cgaattctgg ttcggcgtgt aagcaaacgt
     7501 gccggccgga tcaagtgtca ccgcgaaagg gccgcgtccc gcaacaaaag gagatccagg
     7561 gaccgccaca agcgtgccac tatccgcaat cgtgaaggcc gaaatgttgc cggtgtcccg
     7621 attcgccaca taagccagcg tcccgtcgga gttcagcgtt atgcagaccg gatgcaagcc
     7681 gttctcaatg ggcaccgttg ccgtaagcgc gagcgcgccg ctggcattga tcgaaaatac
     7741 ggagatatcg ttcgaatcgt aatccgccac ataggcgaag tggcccgcgg ggtcaattgc
     7801 gattgatgca ggcgcagttc cggcagcgaa cggtgaaccg aggacttcct tgagcgcgcc
     7861 ggctgcggaa acggcaaacg cggaaatgtc attggaaccg aaattggcca cgtacgcgaa
     7921 ccggcccgcg ggctcgaccg cgatggcgaa tggactgcct ccggccgtga aaggcgagcc
     7981 cgctagcctg gtgaggacgc cactgtccgg atcgattgag aatgccgaga cgtcgttgga
     8041 agcgcgattc gcgacataga cgaactgccc gctgggatgg accatgacat ggaacggatt
     8101 ggtccccgtg gcgaacggcg agccgccaat agccgtgaga gcgccggtcg cggaatcgac
     8161 catgaaggcg gacaagctgt ccccatttgc atttgccacg tatgcaaact tgccggaagg
     8221 catgatggcg atggaaactg cacccgcccc aacgttgact ccaccgttgc tttgtagatg
     8281 tccgctcgtt agagcggtat acgccgaaac cgtaccatcg ccgaaattgg cgacatagac
     8341 aaaatagcgt gccgttccag tctgcaccgg aggcggggtg ggcgtcggcg gcgatgaatc
     8401 gggcggtgga gaaggactct tgaccaccag tggaggcgct ggtgcattgg ccgcagagga
     8461 ggttgtgttt gcgttgttgc cacttccctg cccgcatgct gtaagcagca gcgacaatag
     8521 aagcatcgcc acgtaacgta atccgttttt cttaatgttg ttgtcgaaag cgtcgcatcg
     8581 gctggcgtcc atgccagggc gcagtccatg gtcggttaaa accgctgcgg tagaaaacag
     8641 ggctgaacgt ttgccgacat cgagtgggtc gttttttttc ctgctcttag acatgctttt
     8701 gccagcgtcg agtggttcca gtggtgttgg aaccagcacg aacatcttcc gtgaaggcat
     8761 tcattcctca tcgaggaaga attccaatgt cacattaact ggcgaagtag aacgccgcag
     8821 cagccacgat aggccgaatg caccacgttt acgcgtcggt ggttcttccg gattacatgt
     8881 tgattgtggt tttttgcggt gacgatgaga tcagaacacc agtaccccga ggaatacgcc
     8941 aaagatcaaa accaccagga aaaatgccag ggcaatgaaa aagaggattt tggcaacgcc
     9001 tctggtgccc gccgcgacat ccgtaaaacc gaagaaaccc gcgataaccg atacgataaa
     9061 aaatatggct gcccatttga gcataactgc tcctttcaag atcattgatg gatgcgcttt
     9121 tcatgcagac aagacagtgc gcagtgtggt tcaacggaga tccatggcac ttcgttgtgc
     9181 ataccggtgt acgccaagct gttcattgca actggtaaca gcgcccggcc gccagcacgt
     9241 ggtgatttac ttggccgcta atcaggttta gccttttttg ctctagaccg gagagcgaat
     9301 ggttccattg acgtgtgtgg tggctgttgg tctgccgcaa cggataccac ccgaggaaga
     9361 agaactgatc cgggaagggt atgtcagcgt acggggcgac gttcaattag aagaatatcg
     9421 ccgcagagct tctgatcagg ttggtgtaag catcacactg atgtggagtg acgatggccg
     9481 cccgctctac gcgcgggaat ccatccctcc agttgatctt ctcaacgatt gagcaccggt
     9541 tgtggcggca attaccgccg cggaccttcc agcggttgcg cgcaatcacc gcaaagtgcc
     9601 ggcagcttct gcgcctgttt agctggtaaa aaataccgat tacatttttg cggacatccc
     9661 gggcgttccg caatggtttg aatgttgaaa gaaataggtc gcataagaca tcggcacgag
     9721 ccttgcgttg tagggcgtgc gcgccgaacg gatgcaccgc caccgttcct catccagtaa
     9781 gtgcctcttt gaaagcaagc agtatgaaga ttcggtctgt tgtcgctcgc gggcttttgc
     9841 tcttgagcat cggtgtgatg accggaggct gcgctaccac cgacacaaaa agcagcgctg
     9901 cgaaagcgcc tgacagcacc agcacacggg gtggatccac cggccagca
//
LOCUS       DATMZQ010000097         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2497075, whole genome shotgun sequence.
ACCESSION   DATMZQ010000097 DATMZQ010000000
VERSION     DATMZQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497075"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VN496_02975"
     CDS             <1..475
                     /locus_tag="VN496_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXF15937.1"
                     /translation="KANYIAGTPFVGSDQKMYTGPGGNRGEFAAWEPVEGRKKWSIPE
                     KFPVWSGALVTAGDVVFYGTMEGWFKAVDARTGALLWRFKTGSGIVGQPISYLGPDGK
                     QYVAVLSGVGGWAGSVVSAGIDTRDSTAALGFANAMRDLPSETGMGGMLYVFALP"
     gene            472..1011
                     /locus_tag="VN496_02980"
     CDS             472..1011
                     /locus_tag="VN496_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF15938.1"
                     /translation="MKHRMRALAWACAVALTLCACKREQRAFEQSPSPTELALKSGIS
                     LPSSTPTAKPSGSYEETAYAIAQGKQLFNWFNCSGCHSHGGGGIGPPLMDDKWIYGHE
                     PGEIFATIVEGRPNGMPSFRGRLDDSQVWQLVAYVRSMSALVRRDAIAGRDDGIQKGK
                     PEQRRSRVEPQNSSEAPAQ"
     gene            1017..2033
                     /gene="coxB"
                     /locus_tag="VN496_02985"
     CDS             1017..2033
                     /gene="coxB"
                     /locus_tag="VN496_02985"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXF15939.1"
                     /translation="MHHSGLCLSALLLMARNASASDSQDALMPFGVQAAHIHDLWQVL
                     LWACMVFGVLIVIAVTVALWRAPRSSETTPAEPTPGLHTLSQYVVTGALVVSIIGLLG
                     LTVASVMTDRALAGLSEERPVNVKITSHNWWWEITYEDAQPSRTFMTANELHIPAGRP
                     VIATLESDDVIHSFWVPNLHGKRDLIPGRTAKIKFRADSPGSYRGQCAEFCGYQHAHM
                     ALLVIAEPAEAYEKWADRQRASANTPAEEQKKHGESLFLSSRCAMCHSIQGTSANGKV
                     APDLTHVGSRRYIAAGTLLNGPSQLAAWISDPQRYKPGANMPGTTFSSEDLQALVTYL
                     QSLT"
     gene            2030..>2865
                     /locus_tag="VN496_02990"
     CDS             2030..>2865
                     /locus_tag="VN496_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXF15940.1"
                     /translation="MSSVAELSDTDAAERAALERAWGDRPGLIGWLSSVDHKAIGRRF
                     LVSAFGFFVAAGLLAALMRLQLAIPDNTLVGPDLYNQLFTVHGTTMMFIFAVPVMQAM
                     GLYLVPLMVGTRNIAFPRMNAYAYWLYLFGGLMLYVAFILNIGPDAGWFSYVPLAGPQ
                     YGTGKRSDFWAQLVTFTEVSSLLVAVSIITTAFKLRAPGMALHRLPLFVWALVVTSFM
                     ILFAMPAVMFASTALITDRLVGTHFYNPAEGGDALLWQHLFWFFGHPEVYIIFIPALG
                     MMS"
BASE COUNT          569 a          823 c          868 g          605 t
ORIGIN      
        1 aaaagcaaac tacatcgcgg gcacgccatt cgtgggcagt gaccagaaga tgtacaccgg
       61 acccggcggc aaccgtggag aatttgccgc atgggaaccg gtcgagggcc gcaagaagtg
      121 gagcattccc gaaaaatttc cggtctggag cggtgcgctg gtgacggcgg gcgacgttgt
      181 tttctacggc accatggaag gctggttcaa ggctgttgat gcgaggaccg gcgcgttgct
      241 gtggcgcttc aaaaccggtt cagggatcgt cggccagccg atctcctatc ttggccccga
      301 tggcaagcaa tatgtggccg tcttgtccgg cgtgggcgga tgggcaggat cggtcgtcag
      361 tgctggtatt gacacgcggg acagcacagc cgccctgggt ttcgccaacg ccatgcggga
      421 cttgccgtcc gagaccggta tgggcggcat gctgtacgta tttgcgctgc catgaagcac
      481 cgcatgcgcg ccttggcatg ggcttgcgct gtcgcgctga cattgtgcgc atgcaagcgt
      541 gagcagcgcg ccttcgaaca atcaccttca ccgacagagc tggctctcaa gagcggcatt
      601 tcgctgcctt catccacgcc aaccgcaaag ccgagcggat cctacgagga aaccgcttac
      661 gccatagcgc aaggcaagca gttattcaac tggttcaatt gctcgggctg ccattcgcac
      721 ggcggcggag gaatcggtcc gccgctaatg gatgacaaat ggatttacgg acatgagcca
      781 ggcgaaatat ttgcaacgat tgtagaaggc cggccaaacg ggatgccttc cttccgcggc
      841 cgcctggacg attcccaagt gtggcagctg gtggcctacg ttcgctcgat gagcgcactg
      901 gtccggcgcg acgccatcgc gggccgggat gacggcatcc aaaaggggaa accggaacag
      961 cggcgatccc gcgtcgagcc acagaacagc agcgaggcac cggcacaatg aagcgcatgc
     1021 accacagcgg cttgtgtctg tcagcgctgt tgctcatggc acgaaatgcc tcggcctcgg
     1081 actcgcaaga tgcactcatg ccgtttggag tgcaggccgc tcacattcat gatttgtggc
     1141 aggtcctgct atgggcttgc atggtgttcg gtgtactgat cgtaattgcg gtcacagtgg
     1201 ccctgtggcg cgctccgcgc agcagcgaaa ccacaccggc agaaccaacg cccggtttac
     1261 acacgttgag ccaatacgtc gtgacgggcg cattggtggt ttccatcatc ggcctgctgg
     1321 ggctcacagt cgcgagtgtc atgacggacc gtgcgctggc cggcttatcc gaggagcgcc
     1381 cagtcaacgt caaaatcaca tcacacaatt ggtggtggga aatcacgtat gaagacgcac
     1441 agccctcgcg cactttcatg acagccaatg agctgcatat cccggccggc cgcccagtca
     1501 ttgcgacgct ggaatccgac gatgtaattc acagtttctg ggtgccgaac ttgcacggaa
     1561 agcgtgacct gattccgggc aggacggcga aaataaagtt tcgcgcggac agcccgggca
     1621 gctaccgcgg ccagtgtgcg gagttttgtg gataccaaca cgcgcacatg gcattgcttg
     1681 tcattgcgga gcctgcagag gcatacgaaa aatgggcaga ccggcagcgc gcatcggcaa
     1741 atacacctgc agaggagcaa aagaaacacg gcgaatccct gttcttatcg tcgcgatgcg
     1801 ccatgtgcca ttcaatccag ggcaccagcg ccaacggcaa ggtagcgccg gatctcaccc
     1861 acgttggcag ccggcgctat atcgctgctg gaacgctgtt gaatggcccc tctcagcttg
     1921 cggcctggat ttccgacccg caacgttaca agccgggagc caatatgcca gggaccacct
     1981 tttcatctga agacctgcag gcactggtta cgtacctgca aagcctgaca tgagttccgt
     2041 ggcggaactt tcagataccg atgcagcaga acgcgccgcg ctggagcgtg catggggcga
     2101 tcgtccgggt ctgattggat ggctatccag cgtggaccat aaagcgatcg ggcgccgttt
     2161 cctggtatcg gcattcggtt tttttgttgc tgccggcttg ttggcggcat tgatgcgcct
     2221 gcaacttgcc attccggaca acacgctggt cggccccgat ctgtacaacc agttattcac
     2281 ggtgcacggc accacgatga tgttcatatt cgcggttccg gtcatgcagg caatgggcct
     2341 gtatctggtg ccgttgatgg tcggcacccg caacatcgcg ttcccgcgca tgaatgcata
     2401 tgcatactgg ctctatctgt tcggcggtct gatgctgtat gtggcgttca ttctcaacat
     2461 cgggccggac gccggatggt tcagttatgt cccgctagcc ggacctcaat atggcacggg
     2521 caagcgttcg gatttctggg cgcagctggt tacctttacg gaagtgtctt cgctactggt
     2581 cgcggtttcg atcattacca ccgccttcaa actccgcgct cccggcatgg cgctgcatcg
     2641 gctgccgctg ttcgtatggg cgcttgtggt gacgtctttc atgatcctgt tcgcgatgcc
     2701 ggcagtgatg ttcgccagta cggcactgat caccgaccgg ctggtcggta cgcatttcta
     2761 caacccggct gaaggcggtg atgctctgct gtggcagcac ttgttctggt ttttcggaca
     2821 tcccgaggta tacatcattt tcattcccgc gttgggcatg atgtc
//
LOCUS       DATMZQ010000098         6845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4183926, whole genome shotgun sequence.
ACCESSION   DATMZQ010000098 DATMZQ010000000
VERSION     DATMZQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4183926"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1448
                     /locus_tag="VN496_02995"
     CDS             165..1448
                     /locus_tag="VN496_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF15941.1"
                     /translation="MAWRRGWISLFLFTLAMINYVDRVALSVASKSISEEFGLPPAAM
                     GYLFSSFLWTYLLCLIPMGILVDRLGTKTVNAAGITLWSAATIATGVSWNFVSLIVTR
                     LAMGIGEATTFPAGGRVVREWIPASERGLTNAVFMAGTQAGPAVGALLVAWIISQIGW
                     RGSFLVVGAAGFIWLLAWLIWFGPPEKVRWLGTTERDKILRERDAETSALQKRTSGTR
                     VLDLLRTRTMWGLALAEGCAVYTQYLFLTWLPSYLQSTRGLTIVKTGLFTAVPYLGAA
                     VLGILLGRISDRLLTRDAVGKGRRRTMGIVMMLSSSVILFTPFVDEIWLLLSLFTISL
                     TGMATAISLNFSLVNDLLRNPEDSGKAMSILIVGGNTFGVAAPIITGHVIQATGSYNW
                     AFGIAGMLLVTGATILLTMTRSPIEIPAPAVLARQ"
     gene            1465..2385
                     /locus_tag="VN496_03000"
     CDS             1465..2385
                     /locus_tag="VN496_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXF15942.1"
                     /translation="MVLFNPPIGLQSKVFARLPEELKSADQNNAWVVRQPLGAPVGSL
                     LEGPSFDRQGNFWCVDIQNGRVFKLTRDGVFILVAEYDGWPNGLKFHRDGRAFIADYK
                     HGIMLLDPVNGKVRPFLVEANGVPFKGVNDLFFAANGDLYFTDQGLTGLHDASGRLYK
                     LTPQGKITCLLDNIPSPNGLVMNADETQLYVAVTRGNCVWRVPFVRDGSLAKVGLYVQ
                     LSGGGGPDGLALDEQGGLIVAHVGMGAVWTFSPLGEPAYRIASCMGTHTTNVAFGWPD
                     RDTLYITESSTGTILTAKLAVPGKLMYSHS"
     gene            complement(2396..2923)
                     /locus_tag="VN496_03005"
     CDS             complement(2396..2923)
                     /locus_tag="VN496_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15943.1"
                     /translation="MNDLYHSAPARRDPPPADTLAQTLGWFSIGLGLMEALMPGALLR
                     TTGTKSSKSMIRAYGVREIANGIGILSAKDPTPWIWARVAGDALDITTMASRSGGGGA
                     GVALAAVAGVTALDIICAQNLRTEQQRPREPRRDYSNRSGLPRPPEQMRGAALRGAAS
                     REPALLDATPALSVR"
     gene            complement(2933..4105)
                     /locus_tag="VN496_03010"
     CDS             complement(2933..4105)
                     /locus_tag="VN496_03010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HXF15944.1"
                     /translation="MKALRWHGKKDIRCDDDVPDPIIEDPRDAIIKVTSCAICGSDLH
                     LYDGFMPGMKNGDIMGHEFMGEVLEIGNEVRKLRVGERVVVPFTITCGECEQCKRGNF
                     SVCERSNRNKNLADKVFGHTTAGLYGYTHLTGGYAGGQAEYVRVPYADSGPVKIPDGL
                     SDEQVLFLGDIFPTGWQAAVQCDIEPTDTVAVWGAGPVGQFAMRSALLLGAKQVIAID
                     RVPERLDMAQAGGATPVNFEEESVLECLAEMTNGKGPEKCIDAVGMESHVARSIDSIY
                     DRAKQAVMLESDRPHVLREMIYVCRPAGTLSIPGVYGGLVDKIPFGALMNKGLTVRTG
                     QTHVNRWTGDLLNRIQDGQIDPSFVITHEVGLEDGPDMYQTFRDKEDGCVKVVLKP"
     gene            complement(4336..5403)
                     /gene="mnmH"
                     /locus_tag="VN496_03015"
     CDS             complement(4336..5403)
                     /gene="mnmH"
                     /locus_tag="VN496_03015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HXF15945.1"
                     /translation="MKQQQHTVDAAALDDFDEIVDVRSPAEFIVDHVPGAVNCPVLDD
                     DERARVGTLYKQVSSFEARKIGAALVAKNIARHIDLHFGLRNERWKPLIYCWRGGKRS
                     SSMAHVLREIGWNAATLAGGYQSFRRTVLSQLEQLPSRFEYRVVCGPTGSGKSRLLQA
                     LARHGAQVLDLELLAEHRGSVLGDLPRITQPSQKLFESRLWHALRRLRHGMPVFVEAE
                     SSKIGNLRIPERLLSHMRDAVCIRMEVPVAERVRFLVAEYGRCLAEPGWLKVKLERLA
                     SLHSSATVARWHTLIDAGEWEPLVRDLLHVHYDPAYARSMHTNYVGLAHAHAIDLARL
                     EEKEFTRAVSSLCKEHHVSTA"
     gene            5468..6541
                     /gene="selD"
                     /locus_tag="VN496_03020"
     CDS             5468..6541
                     /gene="selD"
                     /locus_tag="VN496_03020"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXF15946.1"
                     /translation="MLKPAADSEAPEQRVRLTEFSHGGGCGCKISPAVLSEILARTPL
                     RKLHKDLLVGIETGDDAAVYRLNHQQAIVATTDFFTPIVDDAFDFGRIAATNAISDVY
                     AMGGRPIFALALVGMPLDKLPIDAIQQVIAGGESVCAAAGIPVAGGHSIDTLEPIYGL
                     VAIGIAHPANIKRNSSAQAGDILILGKPLGVGILSAALKQRKLSVAGYEQMLATTTQL
                     NTPGVTLAELPGVHAMTDVTGFGLGGHMLGLCRGAGLGAQLRLDEVPVMEEALAWARH
                     GVATGASARNWTSYGHDIVCAQDLPGWQRSLLTDPQTSGGLLVSCAPEVADQVLQIFR
                     DADFARATRIGVMTHGDAVVTLC"
     gene            6594..>6845
                     /locus_tag="VN496_03025"
     CDS             6594..>6845
                     /locus_tag="VN496_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXF15947.1"
                     /translation="MSTQYSARQESATRISCASCKACCCKLEVMLMGDDNPPREFVAT
                     DQWGGQVMQRLEDGWCAAVDRVTMLCRIYERRPTVCRSFR"
BASE COUNT         1292 a         2007 c         2106 g         1440 t
ORIGIN      
        1 ctgggtctgg acggccatgg caaggcactg tcttttttgc tgctgggtga tattgacgta
       61 cgcgtgcctg tagactgtgt gccggaaagt tcgccgtacc gagagcgcct gcgctgaaac
      121 gatcagtgtg agccgagaaa aataaatcgt ttgggaggaa cacaatggcg tggcggcgtg
      181 gctggatcag tctcttcctg tttaccttgg cgatgatcaa ctatgtcgac cgcgttgcat
      241 tatcggtcgc ttccaagtcc atatccgagg agttcgggct gccgcccgcg gcaatgggat
      301 acctgttttc ctcgtttctg tggacttatt tgttatgcct gatcccgatg ggaattctgg
      361 tagaccggct tggcaccaag acagtcaatg ccgctggcat cacgctttgg tcagcagcga
      421 ccatagccac gggtgtctca tggaatttcg tttcgctgat cgttacccga ctggcaatgg
      481 gcatcggcga agcgactacg ttcccggcgg gagggcgcgt agtgcgagaa tggattccgg
      541 ccagcgagcg cggattgacc aatgccgtct tcatggcggg cacgcaggct ggaccggcag
      601 tcggcgcgtt actggtggcg tggatcatca gccagatcgg ctggcgcggc tcctttctgg
      661 tggtgggcgc ggccggattc atctggctgc tggcctggct gatctggttt ggcccaccgg
      721 aaaaggtgcg ctggctaggt acaacagagc gcgacaagat cctgcgcgag cgcgacgcag
      781 aaaccagcgc cctgcaaaag cgcaccagcg gaacacgagt actggatctg ctgcgcacgc
      841 gcacgatgtg gggtcttgcg ctggcggagg gctgtgcggt ctatacgcaa tatcttttcc
      901 tgacttggct gccgagctac cttcagtcca cgcgcggcct gaccatcgtc aagacgggtt
      961 tgttcaccgc cgtgccgtat cttggggccg ccgtgctggg tatccttttg gggcgcatca
     1021 gcgatcgcct gctgacgcga gacgctgtgg ggaaaggccg ccggcgcacg atgggcattg
     1081 tgatgatgtt gtcatcgtca gtgattctgt ttacgccttt cgtcgacgag atctggttgc
     1141 ttttgtcctt gttcaccatc tcgctgactg gcatggcgac cgcaatttcg ctgaatttct
     1201 cgctggtcaa tgatctgctg cgcaatccgg aagattcagg caaagccatg agcatcctca
     1261 ttgtgggcgg caatacgttc ggcgtggcgg cgccgatcat caccggccat gtcatccagg
     1321 ctaccggcag ctacaactgg gcgttcggca tcgcgggcat gctactggtt acgggcgcca
     1381 ccattttgct gaccatgaca cgctcgccga tcgagatccc ggcgcctgct gtgctggcgc
     1441 gccaatagcg ttagcgcagc tgccatcgtg ctattcaatc cgcccatcgg tttgcagagc
     1501 aaggtcttcg cacggctgcc cgaggaattg aagtcagccg atcagaacaa tgcctgggtc
     1561 gtgcgccagc cgctgggtgc gccggtgggt tccctgctgg agggtccgtc gttcgacagg
     1621 cagggaaact tctggtgtgt cgacatccag aacgggcgcg tattcaaatt gacgcgcgac
     1681 ggcgttttca tcctggtcgc ggagtacgat ggctggccca acgggttgaa gtttcatcgc
     1741 gatggccgcg cgttcatcgc cgattacaag cacggcatca tgctgctgga tccggtgaac
     1801 ggcaaagtca ggccgttcct ggtcgaagcg aacggcgtgc cgttcaaagg cgtcaacgat
     1861 cttttttttg ccgccaacgg cgacctctac tttaccgatc aggggctgac cggcttgcac
     1921 gatgcgagcg gccggctata caagcttacg ccgcaaggca aaatcacctg cctgctggat
     1981 aacataccca gtccaaacgg gctggtgatg aatgccgacg aaacgcaact ctacgttgcc
     2041 gtcacgcgtg gcaactgtgt ctggcgggtg ccgttcgtgc gggatggcag cctcgcgaaa
     2101 gtcggcctct atgttcagct ttccggtggc ggcggacccg acggcctcgc actcgatgaa
     2161 caaggcgggc tgatcgtggc gcatgtcggc atgggagcgg tgtggacatt cagcccgttg
     2221 ggagaacccg catatcgcat cgcatcctgc atggggacgc ataccacgaa cgtggcgttc
     2281 ggctggcccg accgcgacac gctttacatc acggagtcgt cgaccggaac catcctgacc
     2341 gcaaagcttg ccgtgcccgg caagctgatg tactcacatt cctgattcgc gtagctcagc
     2401 gcaccgacag ggccggtgtt gcatcgagca aagccggctc gcgcgacgcg gcaccgcgca
     2461 gcgcggcgcc acgcatctgc tccggcgggc gcggaagccc actgcggttg ctgtagtcgc
     2521 gccggggttc gcgcgggcgc tgctgttccg tgcgcaagtt ctgcgcgcaa ataatgtcga
     2581 gtgccgttac accggcgact gccgccaacg caacgccggc accgccgccg ccagaacgcg
     2641 aagccatggt cgtgatgtcg agcgcatcgc cggctacgcg cgcccaaatc cacggggtcg
     2701 gatccttggc ggacaggata ccgatgccat tggcgatctc gcgcacacca taggcgcgga
     2761 tcatggactt gctcgatttc gtcccggtgg tgcgcaatag cgccccaggc atgagggctt
     2821 ccatcaagcc aagaccgatg ctgaaccagc ctagggtttg tgcgagcgta tcggctggcg
     2881 gcggatcacg ccttgccggg gcggaatggt atagatcgtt catgattcgc tcctaaggtt
     2941 taagtaccac tttgacacaa ccatcttcct tgtcccggaa ggtctggtac atatccggtc
     3001 cgtcttcaag cccgacttcg tgcgtgatca cgaacgatgg atcgatctgg ccatcctgta
     3061 tgcggttcag caggtcgccg gtccagcggt ttacgtgggt ctgtccagta cgcacggtga
     3121 gtcccttgtt catcagcgcg ccaaacggaa ttttgtccac cagtccgcca tagacacccg
     3181 gtatggaaag cgtgccggcc ggccggcaca cgtagatcat ttcgcgcagc acgtgcggac
     3241 gatcgctttc gagcatgact gcctgcttgg cgcggtcata gatcgaatcg atggagcgag
     3301 ccacgtgcga ttccatgcct actgcatcga tgcacttttc cggtcctttg ccgttggtca
     3361 tttccgccag acattccagc acgctttcct cttcgaaatt gaccggggtc gctccgccag
     3421 cctgtgccat atcgagccgc tctggcacgc ggtcaatggc gatcacctgc ttggcgccaa
     3481 gcagcaaagc gctgcgcatc gcgaactgtc ctacggggcc agctccccat acagcaaccg
     3541 tatcggttgg ttcgatgtcg cattgcacgg cggcttgcca gccggtcggg aaaatatctc
     3601 cgagaaacag gacctgttca tcggatagtc cgtccggaat ctttactggc cctgagtcgg
     3661 cgtatgggac gcgcacgtat tcagcctgac ctccagcgta gcccccggtc aaatgcgtat
     3721 agccgtacag cccggcggtg gtatgcccaa agaccttgtc ggccaggttc ttgttgcggt
     3781 tgctgcgttc acaaacggaa aaattaccgc gcttgcattg ctcgcactca ccgcatgtga
     3841 tggtgaacgg aaccacaaca cgttcgccga cccggagctt gcgcacctca ttgccgatct
     3901 cgaggacttc gcccatgaac tcatggccca tgatgtcgcc gttcttcatg ccaggcatga
     3961 agccatcgta gagatgcaga tcggaaccgc agattgcgca gctcgtcacc ttgatgattg
     4021 catcgcgtgg gtcttctatg attggatccg gaacatcgtc atcgcagcgg atgtctttct
     4081 ttccgtgcca gcgtaaagcc ttcatgatgg aagcctcctg ttgttgatag ttcgcgcgct
     4141 ggaggcatca gtgcagtgat ttcacccttg aagtccagct cgaacggtac gacagaacgc
     4201 tgtcgggcga gttgcgacgg gccgaaaaaa aagcgggtga tcgcgcacgg cgcgacgatt
     4261 gtaaaaaaaa tcagcggccg gcaatgtttt ctgtcggatt gaaaagcggt gaacagcggc
     4321 tggtaaagtt tgcgttcagg cggtactgac gtgatgttcc ttgcataggg acgataccgc
     4381 acgcgtgaac tctttctcct ccagtctcgc caggtcgatc gcgtgagcgt gcgccagtcc
     4441 gacgtaattg gtgtgcatgg agcgcgcata agccggatcg tagtgaacgt gcagcagatc
     4501 acgcaccagg ggttcccatt cgccggcatc gatcagcgtg tgccagcgtg ccaccgtggc
     4561 gctggagtgc aggctcgcga gacgttccag cttgactttg agccagcccg gctcggccag
     4621 acagcggcca tactcggcaa cgaggaagcg cactcgctct gcgaccggta cctccatccg
     4681 aatgcacaca gcatcccgca tgtgcgacag caggcgttcc ggtatgcgca aattgccgat
     4741 cttgctgctc tctgcttcaa caaacaccgg catgccgtga cgcaaacggc gcagcgcgtg
     4801 ccacagccgg ctctcaaaca atttctgcga aggctgtgta attcgcggca agtcgccgag
     4861 gaccgagccg cgatgttcgg ccagcagttc gaggtcgagc acttgcgcac catgcctcgc
     4921 cagagcttgt agcagccggc tcttgccact gccggtgggt ccgcaaacca cgcgatattc
     4981 aaagcgcgac ggcagttgtt cgagctggga cagtacggtg cggcggaaac tttggtaacc
     5041 gccggctagc gtggcggcat tccagccaat ctcacgcagc acgtgcgcca tcgagctcga
     5101 acgcttgccg ccacgccagc agtagatcaa cggcttccag cgttcgttgc gcaagccaaa
     5161 gtgcaagtcg atgtgacgcg cgatgttttt ggccaccagc gccgcgccga tcttgcgagc
     5221 ctcgaacgag gacacctgtt tgtatagcgt gccgacgcgc gcgcgttcgt catcatccag
     5281 caccgggcag ttcacggcgc ccggaacatg gtcgacgata aactccgcag gcgagcgcac
     5341 atcgacgatc tcatcgaagt cgtccagtgc tgccgcatcg accgtgtgct gctgttgttt
     5401 cacctttggt tgagatccag gcgtccgagt gcggtattct aagcgacccg atccgagagg
     5461 ttagacgatg ttgaagcccg cagccgatag cgaagcgccc gaacagcggg tgcgcctgac
     5521 cgagtttagt cacggcggcg gctgtggctg caagatttcg ccagcggtgc tatccgagat
     5581 tctcgcaagg acgccgctgc gtaaattgca caaagatctt ttggttggca ttgaaaccgg
     5641 cgacgatgca gcggtatacc ggttgaatca ccagcaggcc attgtcgcaa ccacggactt
     5701 tttcacgccc atcgtggatg atgcgttcga tttcggtcgt attgcggcga ccaacgccat
     5761 ttcggacgtg tatgccatgg gcggtcgccc gattttcgcg ctcgcgttag tcggcatgcc
     5821 gctcgacaaa ctacctatcg acgcgatcca gcaagtgatt gccggcggtg aatcggtttg
     5881 tgctgcggcg ggcatcccgg tcgcgggcgg ccattcaatc gatacgctgg agccgatcta
     5941 cggtctggtc gcgattggca tcgcgcatcc ggcaaacatc aagcgcaaca gtagcgcgca
     6001 agcgggcgat atcttgattc tcggcaagcc gcttggcgtt ggcattcttt ccgctgcgct
     6061 caagcagaga aaactttccg ttgccggtta tgagcagatg ctcgctacca cgacacagct
     6121 caatacgccg ggcgttacac tcgcggaatt gccgggtgtc catgccatga ccgatgtgac
     6181 cggattcggt ctgggtggac atatgcttgg cctgtgtcgg ggcgccggac tgggcgcgca
     6241 actgcgcctc gatgaggtgc cggtgatgga agaggcattg gcctgggcca ggcacggggt
     6301 tgccacgggc gcttccgcgc gtaactggac ttcatatggc cacgatattg tttgcgcgca
     6361 ggatctgcca ggctggcaac gcagcctgct gaccgatccg cagacttcgg gcggcctgct
     6421 ggtgtcgtgc gcacctgaag tggcggacca ggtactgcag atcttcaggg atgcagattt
     6481 cgcgcgcgcg acaagaatcg gcgtcatgac gcacggcgat gccgtcgtga cgctatgcta
     6541 accacgatgc aaaaagccag gcgtgatgtg gcgtttaccg cggtcgatcg gccatgagca
     6601 cgcagtacag tgcgcgacaa gagtctgcca cgcgcatctc ttgcgcatcg tgcaaggcct
     6661 gttgttgcaa gctggaagtc atgctgatgg gtgacgataa tcccccgcgc gagtttgtcg
     6721 cgaccgacca gtggggtgga caggtcatgc aacggctgga agatggctgg tgcgctgctg
     6781 tggatcgcgt cacgatgctg tgccgcattt acgagcgccg gcccacggtg tgccgctcgt
     6841 tcagg
//
LOCUS       DATMZQ010000099        15782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1808986, whole genome shotgun sequence.
ACCESSION   DATMZQ010000099 DATMZQ010000000
VERSION     DATMZQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15782
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808986"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VN496_03030"
     CDS             complement(<1..173)
                     /locus_tag="VN496_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HXF15948.1"
                     /translation="MAYDSLMVFTGNAHPRLAEDVVKHLNIPLGRAMVGRFSDGEIQV
                     EIMENVRGKDVYLV"
     gene            complement(304..378)
                     /locus_tag="VN496_03035"
     tRNA            complement(304..378)
                     /locus_tag="VN496_03035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Gln,seq:ttg)
     gene            complement(386..1228)
                     /gene="ispE"
                     /locus_tag="VN496_03040"
     CDS             complement(386..1228)
                     /gene="ispE"
                     /locus_tag="VN496_03040"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXF15949.1"
                     /translation="MNPAAVVFPAPAKLNLFLHIIGRRQDGYHMLQTAFRFIDYGDDL
                     HFSVRADGVIRQLNPLPGVPAEQDLTVRAARLLQQETQSPKGADIGIVKRIPLGGGLG
                     GGSSDAATTLIALNRLWRTGVSRARLQQLALQLGADVPVFVFGQNAFAEGVGERLQVL
                     GVPPAWYLVLVPELTVSTAEIFSAAELTRNTNAITIAAFSVGQGHNDLEAVVCRRYPQ
                     VARHLEWLHQYGDARMTGSGACVFCAFAGEVLARRAWAQLPADMRGFVARGLERHPLW
                     SEDD"
     gene            complement(1225..1797)
                     /gene="lolB"
                     /locus_tag="VN496_03045"
     CDS             complement(1225..1797)
                     /gene="lolB"
                     /locus_tag="VN496_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HXF15950.1"
                     /translation="MQHRLGVSALCAVVAVLSGCAIRPVQNLPASNEGIAAFSLNGRV
                     AVKLENRGYTASLRWRHTAAHDSLKLLSPVGSVVGQIEADAGGATLTTGDKKVYRSSD
                     AQSLTRDVLGWDLPLAGLRYWVTGRIDPAAPVESVERDEQQRLKSLMQSEWRIAYLEY
                     FGDSALPSRISLAYDRLSLRLIVEQWDLTQ"
     gene            complement(1806..3599)
                     /locus_tag="VN496_03050"
     CDS             complement(1806..3599)
                     /locus_tag="VN496_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF15951.1"
                     /translation="MPGLSRSLATLIIALCILLGACAQREIVAARDATHFAAADADLS
                     AQAQPESLDSRAREVKVLAVADLPERALDSELLYKFLLAEIAVQHGHYQVAAQTYLDL
                     AKSTRDPRIARRATEVALYGRYTDAALEAAKLWLAAEPDSTAARQTLAALYVNSNDLQ
                     AAKPLLQQMLAADGDNIGESLAQLYPLLAKHDDKIAVLALVKELTQPYRDRPEAHLAM
                     AEASLGADKYDAALSEIREAMRLRPDWESGALFQAQVLNRESRAKALNYLSGFLATYP
                     KAQEVRLGYARQLINDKKFAEARAEFQRLLDDNPNNSDLAVTVALLSVQMNDLDVAET
                     QLKRVLDMDYKDPEAVRFHLGQIAEERKRYDEAAKWYLSIETGEQYVAAHSHYAFMLA
                     KQGKLAEARDHLRGLEPQNDAQRIQLLQSEAQLMREAKDYQESFNILNKALKAQPDQP
                     DLLYDVALAAEKLDRIDIAEANLRRLIVLKPDHAQAYNALGYTLADRTDRLTEARGYI
                     EQALKLSPEDPFILDSMGWVEYRLGHVSQGLQYLERAFQQRPDPEIAAHLGEVLWAKG
                     DKPGAEKVWRDAFRDNPENDELQKVITKYLR"
     gene            3828..4784
                     /locus_tag="VN496_03055"
     CDS             3828..4784
                     /locus_tag="VN496_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF15952.1"
                     /translation="MTNTASSELTVSARSLSRSYGSNRAVREVSLQLMRGEVLGLLGP
                     NGAGKTTTMQMLTGNLAPSSGEVDICGIDLIDQPTRAKSRIGYLPEIPPLYRELKVDE
                     YLNLAARLHKVKKNALRKAIERAKSRCGLADMGSRLIGALSKGYQQRVGIAQAIIHEP
                     DVIILDEPTIGLDPNQIREIRILIRDLGRDHSLILSTHILPEVEAVCDRVQILHRGEV
                     VFNDSIAALKQFRGGQTLQLGLRTPPPTEEISRIAGGASVDAVNANLFRVHPSSGADP
                     TEDLVRASVERGWGLFQLQPAQASLEEVFVQLTHREEEEVSA"
     gene            4781..5524
                     /locus_tag="VN496_03060"
     CDS             4781..5524
                     /locus_tag="VN496_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXF15953.1"
                     /translation="MIQTIVAKELRALFTSPLAWVILAFLQIILAWIFLTRVDYFLTI
                     QPQLARVPNAPGLSEIVVVPMFGIASIVLLMSVPLLTMRLIAEERRNRTMTMLMSAPV
                     SMTQIVLGKFLATAIFLAIVGGLISLMALSLLMGGKLDYGLLGANLLGLLLLTSSFAA
                     IGLFVSCLTTHPVVAAIGALGAFLVLWLINIAASDPGSPVHLFSLLKHFEGFMNGTIG
                     LSDLIYFAVLGAVFLVLSIRRLDADRLRA"
     gene            5521..6873
                     /locus_tag="VN496_03065"
     CDS             5521..6873
                     /locus_tag="VN496_03065"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HXF15954.1"
                     /translation="MKLNRKLRLQLLIQNGLFVVLLIAFALGILWVTKDIKTQWDLTQ
                     GQRNTLSQASIDVLKQVTGPVNVTAYATAQDAEGDARKLVSTFLGSYQRAKKDFLVTY
                     VDPREQPQKAQAAGVRANGELVVEYNGRSEHLTNLTEQDMTNLLLRLVRSAERQVSYL
                     DGHGERKLDGRANHDLGDFGSQLSVKGFKIAALNLAIAPEVPDNVAVLVIASPRVNLL
                     PGEVSKIKRWIEKGGNLLWLVDNDSLHGMQAVADELGLNLTPPGTVIDPAAGGLKLPA
                     TFALATGYGQHQITERSTITSVFPYARRIAANEGSKWRFTPLVNVAQGGWLETGNIDG
                     NVSFDPKTDIRGPITIAAALERSVGDRKQRVVVTGSANFLANQYIGTLGNLDLGINMM
                     NWLAGDEALITVQPRTRSDLTLELPRAGLAMMGFGFLLVLPLVLLFCGGVIWWRRRKA
                     "
     gene            6870..6995
                     /locus_tag="VN496_03070"
     CDS             6870..6995
                     /locus_tag="VN496_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15955.1"
                     /translation="MKYKVLINLALIAALVALSLLAYFKPWQQATPAIKLSSLKR"
     gene            6999..7727
                     /locus_tag="VN496_03075"
     CDS             6999..7727
                     /locus_tag="VN496_03075"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXF15956.1"
                     /translation="MTRITVEPRRAGVIRLEKRDGAWRMVAPFDAQADGPPVDRLLDI
                     VNATAKQKLANADLAQFDLDPPAIRVGLNDEVFSFGRVNDITYEQYVATATGVYLVPP
                     LYGYNIPTDAAKLLSRRILDQGEIPVAFDFGRYRIVRDDKGTWAVTGILPGAKDKPLS
                     QDDFNRWADEWQHTSALSVEPDKSSHSRDQLVVRFKNGKSVSMAILQKAPDFQLVRND
                     NRMRYHFGVDVGQRLLDPYVVAKK"
     gene            7736..8554
                     /gene="mutM"
                     /locus_tag="VN496_03080"
     CDS             7736..8554
                     /gene="mutM"
                     /locus_tag="VN496_03080"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXF15957.1"
                     /translation="MPELPEVETTRRGLVQHMQGQRIATAIIRNGSLRWPIPRDFRRR
                     VAGRMVRTVERRGKYLLFDCETGWIMLHLGMSGSLQVVPGAATAGKHDHLDLVLDSGV
                     VIRFNDPRRFGAVLWEPGDPLRHRLLTDLAPEPLGTDFTAAWLHKHTRTRNAAIKSLV
                     MDSHIVVGVGNIYASESLFRAGIHPAMAARRLSLLRCEKLVAAIQQTLQAAIEAGGST
                     LRNYVGAEGRPGYFQKSYFVYGRAGHPCQVCVTPIKAMRQGQRSTFYCPVCQRR"
     gene            8833..10578
                     /locus_tag="VN496_03085"
     CDS             8833..10578
                     /locus_tag="VN496_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXF15958.1"
                     /translation="MTSTKRYLRIALAAAFSAAVAAGSSLAADVDGARVQNADKELEK
                     GNWLTYHGSYKSWHYSPLDQINTKTVGKLSEAWSHTASRANRGLQGFPLAIDGILYYS
                     SPYNQVYALDGATGQMLWTYKQKLNEDLVARQTHSPYNRGIAAGYGNIFIGTLDGKLA
                     AIDMKTGKLSWETKLIDSEKLTVGFTGAPLLVKDKVIIGSQGGEWPYRGPIFGVNAKT
                     GEKVWQFFTVGGNDGSASDKRDTWGNDSWKVGGGGGWMAGAYDPETNSVWWGTANPAP
                     LYDWSGDKWMTEGARPGINLYITSVILLDPDTGDLKSYHQELPHDAWDFDSSMGEFVF
                     LERDGKKYVVHPNKGGFVFVYDRNGKPVNVYKGVDAITFVKDIKPDGTLVGRQDYTEG
                     KHENLCPAIAGGYSWNAGTYNPKTGLFYRVGYEWCIDFTVVKTEPITEPVVQLNIGAD
                     FTFVPPKGKKSMGGHIRARDPISGKVKFEIPYVGATPHASLLSTGGNVLFVPEADGTL
                     AAFDATNGKKLWAHNDGQGHNGGIITYLAKGKQYVAVMTGWGSLVGDGYGDWYGEPWK
                     SMPKDSGVLKVFALQ"
     gene            10745..11107
                     /locus_tag="VN496_03090"
     CDS             10745..11107
                     /locus_tag="VN496_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF15959.1"
                     /translation="MTTTKTYLQVRCLLLTTVLGFALCFTAQAQEQKAAAKHLDVKST
                     FRNICGFCHENYGRKAAKGPQLMNSERSDEFIFNRIKNGMPGRMAAFGTAFTDDQIRD
                     IIKFIRSLKPDEEPQNPS"
     gene            11104..11889
                     /locus_tag="VN496_03095"
     CDS             11104..11889
                     /locus_tag="VN496_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXF15960.1"
                     /translation="MTFNLPQRAGYAFVCVALLLIAANTAGARTLAEIKSLGGISMCA
                     NRDALPYASNKPETPGFQIEIGRAIAEGLGVPLSIEWILPRRRANVVNCDMLLDNIND
                     AEVNEGRMRLSRPYQKSGLALGLRNDSELVNDFTELKKGQKIGVMINSYAAMVLGKAG
                     KTFSPYAFQSDMVEDLQKGELYGAAVSAATMSYYIRQHPESGLRLVHAFDSVPQLTWE
                     VSVGLRKADAALVDAVNEILDKLIADGTLTSIYEKYGVEHRIP"
     gene            complement(11901..12170)
                     /locus_tag="VN496_03100"
     CDS             complement(11901..12170)
                     /locus_tag="VN496_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HXF15961.1"
                     /translation="MALLITDECINCDVCEPECPNNAISQGVDIYQIDPDKCTECVGH
                     YDRPQCQEVCPVDCIPLNPDCVESQEQLYQKYLSLTERKVKASAS"
     gene            complement(12173..12655)
                     /gene="coaD"
                     /locus_tag="VN496_03105"
     CDS             complement(12173..12655)
                     /gene="coaD"
                     /locus_tag="VN496_03105"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXF15962.1"
                     /translation="MNRAVYPGTFDPITRGHEDLVRRASNLFDEVLVAVADSRGKRPF
                     FDVDERVAMAKEALAAYRNVRVEGFSGLLMQFLQKHDAKIILRGLRAVSDFEYEFQMA
                     GMNRKLYPDVETLFLTPAEQYMFISATMVREIVSLGGDVSPFVNPLVIPRMKARLERG
                     "
     gene            complement(12664..13209)
                     /gene="rsmD"
                     /locus_tag="VN496_03110"
     CDS             complement(12664..13209)
                     /gene="rsmD"
                     /locus_tag="VN496_03110"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXF15963.1"
                     /translation="MKTNQVRIIGGQWRSRLVRFPEAQSLRPTPDRLRETLFNWLGQD
                     LTGKSCLDLFAGSGALGFEAASRGARPVVMVEHNTGVYHALRKTRALLGAEQVELYHA
                     DTFSFLKTDVRRYDVVFLDPPFRHGLMPRVMAELPGHLAAHACVYAEAEEPIGVPDAF
                     DLLKQTRVGQVHGLLIKCRSS"
     gene            complement(13285..14613)
                     /locus_tag="VN496_03115"
     CDS             complement(13285..14613)
                     /locus_tag="VN496_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXF15964.1"
                     /translation="MTSASQYLFKGLAGLCLAAACTAHATLPIQEWQTANGARVLFVE
                     THDLPMFDLAVDFPAGASRDLRDKSGLASLTLELMRSGAGDMSEGEIAERLASIGADM
                     SERFDLDRAGYGLRSLSSARERDESLKILATVLQKPTFPQPILEREQQRTLAALKEAE
                     SRPEVLAEREFKHLVYGQHPYSQRGAGELNTVAKLQRDDVVNFYRQHYVADHATVSII
                     GDLTRKEASAIAELLTVGLPSKSQRVALLPPVQQLKSAQLSRIPHPATQAHILIGQPG
                     IKRLDPDYFALFLGNQVLGGGGFASRLNEEIRQKRGYAYSAYSYFNPLALEGPFQIAL
                     QTKSEQAEQALKVAQDTLANFVAEGPTQQELDAAKQNIIGGFALRIDSNKKIQDYLAI
                     IGFYRLPLTYLDDFPVKIEKLTLEQVKDAWRRRIYPDRMVTVIVAGTEAH"
     gene            complement(14600..>15782)
                     /locus_tag="VN496_03120"
     CDS             complement(14600..>15782)
                     /locus_tag="VN496_03120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXF15965.1"
                     /translation="VAHMLEHMMFKGTKSVPAGEFSRTIARAGGRENAFTSHDYTAYY
                     QQLHNSKLSLALELEADRMSNLNFAQDEFAREMNVVMEERRSRTDDNPHAQVHEQMMA
                     TVYMAHPYRTPIIGWMNDLQNMQLADARAWYEKWYTPNNATLVVAGDVKPDDVFKLGE
                     KYFGALPSRTPPARKVQTEPPQAGIKRIIVKAQAELPYVAMAYHAPSLRDVEHDWEPY
                     ALAVLSGILDGSDASRLNRELVRKMRVADSANANYDMLNRGPALFFLDGVPAAGKTVQ
                     DVEAALREQIRLLVTEGVSNDELMRVKAQVTAAQVYARDSVYYQAMRIGMLQTIGLPY
                     DSSDLQVRKLQQVTAEQVRDVARKYLVDDNLTVAVLDPQPLPAGKRPRPNPEGGARDQ
                     R"
BASE COUNT         3452 a         4494 c         4456 g         3380 t
ORIGIN      
        1 accaggtaga cgtccttgcc gcggacgttc tccatgatct cgacctggat ctcgccgtca
       61 ctgaagcgcc cgaccatcgc gcggcccagt ggaatgttga gatgttttac cacatcctca
      121 gccaacctcg gatgcgcatt gccggtaaac accatcaaac tgtcatatgc catctctagc
      181 cactcgattc cgaaactgga gtctggtcaa ccacgcacaa cgctggtcca acacataaat
      241 agcagcgaag caatgcgctc gcggccgccg ttaccagtct ccagtcctgc taaggctcca
      301 gactggctgg ggaggtaggg atcgaaccta cgaatgccgg aatcaaaatc cggtgcctta
      361 ccacttggcg actccccaaa aaactctagt catcctccga ccacaaagga tgccgctcca
      421 agccccgcgc cacaaaaccc cgcatgtcgg ccggcaattg tgcccacgcg cgccgggcga
      481 gtacttcacc ggcgaacgcg caaaagacac atgctccgga tccggtcatg cgagcatcgc
      541 catattggtg caaccactca agatggcggg ccacctgcgg gtaacgccgg cacaccacgg
      601 cttcgagatc gttgtggccc tggccgactg aaaaggccgc tattgtgatt gcattcgtat
      661 tacgtgtcaa ttcagctgcg gagaagattt ctgcagtgga aacagttagt tcgggtacca
      721 gaacgaggta ccacgcgggt ggcacaccga gtacttgcaa gcgctcgcca acaccttctg
      781 caaatgcatt ttggccgaat acaaaaaccg ggacatcagc acccaattgc agcgcaagtt
      841 gctgcaagcg tgcgcgcgat acgccggtgc gccacaagcg gtttaatgca atgagagtcg
      901 tcgcggcgtc tgaactgccg ccgccgagtc cgccgcccaa gggtattcgt ttgacgatac
      961 cgatatctgc ccctttcggg gactgcgtct cctgctgcag aaggcgagcg gcgcgaaccg
     1021 ttaaatcctg ctcggcaggc acccctggca aaggattgag ctggcgtatg acgccatcgg
     1081 cacgcacgga aaagtgcagg tcgtctccgt agtctataaa tcggaacgcc gtctgcaaca
     1141 tgtgatagcc gtcttgccgg cgtccaatga tatgaagaaa gagattaagt tttgcggggg
     1201 ccggaaaaac aactgcggcc ggattcactg cgtcagatcc cactgctcga caatgagtcg
     1261 caaactgagc cgatcatatg caagcgaaat gcgcgaggga agcgcactgt caccgaaata
     1321 ttcaaggtag gcgatacgcc attcgctttg catcaagctt ttgaggcgtt gttgctcgtc
     1381 tcgctccaca gactctacag gcgcagctgg atcaatccgg cctgtaaccc agtaacgcag
     1441 acccgctagc ggaagatccc aaccgagcac gtcacgtgtc agtgactgcg catcgctaga
     1501 tcgatagact tttttatctc cggtggtgag cgttgcgccg ccggcatcgg cctcgatttg
     1561 gcctacaact gaaccgaccg gagagagcag cttgagcgag tcatgcgctg cggtatggcg
     1621 ccagcgcaaa cttgccgtgt aaccccgatt ctccagtttg acggccaccc ttccgttaag
     1681 ggagaaggct gcgatgccct cattggaagc cggcagattt tgcaccggtc ggatcgcgca
     1741 tccgctcaat accgcaacga ctgcgcatag cgccgaaaca ccgagccgat gttgcatgtt
     1801 tcgacttaac gcaggtattt ggtaataacc ttttgcagtt cgtcgttttc cggattatcc
     1861 cgaaacgcat cacgccatac tttttccgcg cccggtttgt cgcccttggc ccacagcacc
     1921 tcgccaagat gggcggcaat ctcaggatcc ggccgctgct ggaatgcgcg ttccaggtac
     1981 tgcaagccct gtgacacatg acccagtcga tactccaccc accccatgct gtccagaatg
     2041 aaggggtctt ccggcgacag cttgagcgcc tgttcgatgt agccacgcgc ctcggttagc
     2101 cggtcggtcc gatctgcaag cgtataacca agcgcgttat aggcttgcgc gtggtctggt
     2161 tttagcacga tcaaacgccg caagttggcc tcggcaatat caatacgatc aagtttttcg
     2221 gccgctaaag caacgtcgta gagcaagtca ggctggtctg gctgagcctt taacgccttg
     2281 ttcaggatat tgaacgattc ctgatagtcc ttcgcctccc gcatcaactg cgcctcggat
     2341 tgcagcaact ggatgcgctg tgcatcgttt tgcggttcca ggccgcgaag atggtcgcgc
     2401 gcttcagcaa gcttgccctg tttggccagc atgaatgcat aatgcgaatg ggcggctacg
     2461 tattgctcgc ctgtctcgat cgataaatac catttagccg cttcgtcgta gcgtttgcgc
     2521 tcctcagcga tctggcccag gtgaaaacgc accgcctcgg ggtccttgta atccatgtcc
     2581 agcacgcgct tcagctgcgt ctccgcgacg tccaggtcat tcatctggac ggacaataga
     2641 gcaacagtca cggcgagatc actattgttc ggattgtcat caagcagtcg ctgaaactcc
     2701 gcgcgcgctt ccgcgaattt tttgtcattg atcaattggc gggcatagcc gagtctcact
     2761 tcctgcgctt tcgggtaagt ggcaaggaag ccgctcaaat agttcagcgc tttggctctc
     2821 gactcccgat tcaatacctg cgcctggaac agggcgccac tttcccagtc cgggcgcaga
     2881 cgcatcgctt cgcggatttc cgaaagcgcc gcatcgtatt tatcggcgcc tagtgaagcc
     2941 tctgccatcg cgagatgcgc ttccgggcga tcacggtacg gctgggtcag ctctttaact
     3001 agcgccaaca ccgctatctt gtcgtcatgc ttggccagca gaggatacag ttgagcgagc
     3061 gactcgccga tattgtcgcc gtccgcagca agcatttgct gcaacaacgg tttggctgcc
     3121 tgcaaatcgt tggagttcac atagagagcc gccagcgttt gccgcgcggc ggtcgaatcc
     3181 ggttcggcag cgagccacag ctttgccgct tccaatgccg catctgtgta gcgaccgtaa
     3241 agcgccactt cggtagctcg ccgagcgatt cgcggatcgc gcgtggattt ggcgagatcg
     3301 agataggtct gcgcggccac ctggtagtgg ccgtgctgga cagcgatttc cgcaagcaga
     3361 aacttataca gcagttcgga atccaacgcg cgctctggca gatccgctac agccagaacc
     3421 ttcacttcac gggcgcgtga gtccaggctt tccggctgcg cctgtgcgga caggtcggca
     3481 tcggcggcag cgaagtgagt agcatcacga gcggccacga tctcacgttg tgcacaagcc
     3541 ccgagcagaa tacacaaggc aatgatcagg gtggcgagac tgcgcgagag acctggcatg
     3601 aatataggga ggctatgaag acgcggaaaa ccccagacga agtattagag atttctttcc
     3661 tgcgcgaagt tcttcaatcc agtggacagt ttaggtatat ttggcgccca tcacaagtga
     3721 cccattgcgc cgcaccggcc cccgcaccgg tgatgcgcgc aatgctggat cgcatatggg
     3781 ccaaagagca gacagcagat ttccaaattc atcgataagg caagggcatg accaatacgg
     3841 caagctcaga attgactgtt tccgcgcgat cgctttcgcg ctcctatggc agtaatcggg
     3901 cggtacgcga agtatctctg caactcatgc gcggcgaagt gctcgggctg ctgggtccaa
     3961 acggcgcggg aaagaccacc accatgcaga tgctgacggg caacctggcc cccagcagcg
     4021 gcgaagtaga tatctgtggt atcgatctga ttgatcagcc gaccagggca aagtcgcgca
     4081 tcggctacct gccggagatt cctccgttgt accgggaact caaggtcgac gagtacctga
     4141 accttgctgc gcgtttgcac aaggtgaaga aaaacgcgct gagaaaagca atcgagcggg
     4201 ccaagagccg ctgtggtctg gccgatatgg gcagtcggtt gatcggcgcg ctgtcgaagg
     4261 gataccagca acgggttggc attgctcaag ccattattca tgagccggat gtcatcatcc
     4321 tggacgaacc gactatcggg cttgacccga accagatccg cgagatccgc atcctgatca
     4381 gagatctggg acgcgaccac agcctgatac tgtcgacaca catccttccg gaagtcgaag
     4441 cggtgtgcga ccgcgtgcaa atcctccacc gcggcgaagt cgttttcaac gacagtatcg
     4501 ctgcgctcaa gcagtttcgt ggcgggcaga cgctgcagct tggtttgcgt actccgcccc
     4561 caaccgagga aatcagccgc attgccggcg gagcttcagt cgacgctgtc aacgccaatc
     4621 ttttccgggt tcatccgagc agcggtgccg atcccaccga agatttggtc cgtgcctcgg
     4681 tcgagcgcgg ctggggcctg ttccagctgc agcctgctca agccagcctc gaagaagtat
     4741 tcgtacaact gacccaccgc gaagaagagg aagtttccgc atgatccaaa ccatcgtcgc
     4801 gaaagagttg cgtgctttat ttacctcacc gctggcctgg gtgattctcg cgttcctgca
     4861 aatcatcctc gcctggatat ttctcacccg ggtggactat ttcctgacta ttcagccgca
     4921 attggcgcgc gtgccgaatg ccccgggttt gagcgaaatc gtggtcgtgc cgatgttcgg
     4981 tatcgcatcc attgtgctgt tgatgtcggt gccgctgctg acaatgcgcc tcattgccga
     5041 agagcgccgc aacaggacga tgaccatgct tatgtcggcc ccggtttcca tgacgcagat
     5101 cgtgctcggc aaatttttgg caacagcgat ttttctcgcc attgtcggtg gtctcatcag
     5161 cttgatggcg ttgtcgctgc tgatgggggg caagcttgac tacggactgc tgggcgcaaa
     5221 tctgcttgga ttgcttttgc tcaccagcag ctttgccgcg attggcttgt tcgtttcatg
     5281 cctcacaaca catccagtgg tcgctgccat cggcgcactg ggggcctttc tggtgttatg
     5341 gctgatcaac atcgccgcgt cagatccggg cagtcccgtt catttgtttt cgctgctcaa
     5401 gcatttcgag ggcttcatga atggcaccat cggcctgagt gacctgatct attttgcggt
     5461 gctcggcgcg gtatttctcg ttctttccat caggcgtctc gacgcagacc ggctacgtgc
     5521 atgaaactga atcgcaaact tagattgcaa ctgctgatac aaaacggctt gttcgtggtg
     5581 ctgctgatcg cctttgcgct tggcatcctg tgggtcacaa aggatatcaa aacgcaatgg
     5641 gatctgaccc agggtcagcg caatacgttg tcgcaggctt ccattgatgt gttgaagcaa
     5701 gtaaccggac cggtcaacgt gaccgcttat gcgactgcgc aagacgccga gggagatgcg
     5761 cgcaaactgg tcagcacgtt tctgggcagt tatcagcgtg caaagaagga tttcctggtg
     5821 acttatgtcg atccgcgcga gcaaccgcag aaggcacagg cggccggcgt acgcgccaac
     5881 ggtgaactgg tggtcgaata caacggccgc agcgagcatc tgaccaacct gacagaacag
     5941 gacatgacca atttgctgct taggctggtg cgttccgcgg aacgccaggt gagctatctc
     6001 gacgggcatg gcgagcgcaa gctcgatggt cgcgcaaacc atgatctcgg cgatttcggc
     6061 tcacaattga gcgtcaaggg attcaagatt gccgcgttga acctggccat tgccccggaa
     6121 gtaccagaca acgtggccgt gctggtaatc gccagcccgc gtgtgaatct gctgcccggg
     6181 gaagtcagca agatcaagcg ctggatcgaa aaaggcggca acttgctgtg gttggtggac
     6241 aacgattcct tgcatggcat gcaagccgtt gcggacgaac ttggactcaa ccttacgccg
     6301 cccggcactg tcatcgatcc ggcagcgggc ggactgaagt tgccggccac attcgcactg
     6361 gcgaccgggt acggccaaca tcagatcacc gaacgatcga cgatcaccag cgtatttcca
     6421 tacgcgcgcc gcatcgccgc gaacgagggt tccaagtggc gtttcacacc tctggtcaac
     6481 gtggcacagg gtggctggct tgaaactggc aatatcgacg gtaatgtttc attcgatccg
     6541 aaaacagaca ttcgcggtcc gatcactata gctgccgcgc tggaacgcag tgtcggcgat
     6601 cgcaagcagc gggttgtggt caccggaagc gccaattttc tggccaatca gtacatcggc
     6661 acccttggca atctcgacct gggcataaac atgatgaact ggctcgccgg tgatgaagcg
     6721 ctgatcacgg tgcagccgcg tacgcgttct gatttgacgc ttgaattacc gcgtgccggc
     6781 ttggcaatga tggggtttgg ttttctgctt gtgctgccgc tggtgttgct cttctgcggc
     6841 ggcgtcatct ggtggcggcg gcgcaaggca tgaaatacaa ggtcctgata aaccttgcgc
     6901 tcatagcggc gctggtggcg ttgtcgctgt tggcgtattt caagccgtgg cagcaagcaa
     6961 cgcccgcaat caaattatcg tctttgaaac gctaggacgt gactcgcatc acggttgaac
     7021 cgcgccgggc gggagtcatc aggctggaaa agcgtgacgg cgcatggcgc atggttgcgc
     7081 cgttcgacgc tcaggccgac ggtcccccgg tcgaccgcct gctcgacatc gtcaatgcca
     7141 ccgcgaaaca aaaattggcc aatgcagatc ttgcgcaatt cgatctggat ccaccagcca
     7201 ttcgcgtcgg cctgaacgac gaggtctttt cgtttggacg agtgaacgac attacttacg
     7261 aacaatatgt cgctaccgcg accggtgtct acctggtgcc gccgctgtat gggtataaca
     7321 ttcccaccga cgccgccaaa cttctaagcc ggcgcattct tgatcaggga gaaataccgg
     7381 tggccttcga ttttgggcgc taccggatag tgcgcgacga caagggaacc tgggcggtta
     7441 ccggtatcct cccgggtgcg aaagacaagc cgctatcaca ggacgatttc aaccggtggg
     7501 ccgatgagtg gcagcatacc tcggcgcttt cggtggaacc cgacaagagc agccactcgc
     7561 gagaccagct ggttgtccgc ttcaaaaatg gcaaatccgt ctccatggcc attctgcaga
     7621 aggcgccgga ttttcagttg gtacgcaacg acaaccggat gcgctaccac ttcggcgtcg
     7681 atgtcgggca gcgtctgctc gacccttacg tcgtcgcaaa aaaataacga ctcgcatgcc
     7741 agagctgcct gaggtggaga ccacacgccg cgggcttgtg cagcatatgc agggccagcg
     7801 cattgccact gcgatcatac gtaacggctc gctgcgatgg ccaataccgc gcgatttccg
     7861 gcgcagggtt gccggtcgca tggttcgcac agttgaacgg cgcggaaaat atctgctgtt
     7921 cgattgcgaa acaggttgga tcatgctgca tctgggcatg tccggaagtt tgcaagtcgt
     7981 tccaggcgca gcaacggccg gcaagcatga ccatctcgat ctggtgctgg actctggcgt
     8041 ggtcatcaga ttcaacgacc cacgtcgatt cggtgcagtg ctctgggagc ccggcgatcc
     8101 attgcgtcat cggctgctga ccgacctggc accggaacca ctgggaacgg atttcactgc
     8161 cgcctggctg cataaacata cgcgcacgcg gaacgcggcc atcaaatccc ttgtcatgga
     8221 cagccacatc gtcgtcgggg tcggcaacat ttatgcgagc gaatcactgt ttcgtgcggg
     8281 catacacccg gctatggcgg cccggcgcct gtctttgctg cgttgcgaaa aactcgttgc
     8341 agcgatccag cagactcttc aagcggctat agaggctggc ggtagcacgc tgcgcaacta
     8401 cgtcggcgcg gaagggcgtc cgggatactt ccagaaaagc tatttcgtct atgggcgggc
     8461 gggtcaccct tgccaggtgt gcgtcacacc gatcaaggca atgcgtcagg ggcaacgatc
     8521 gactttctat tgcccggtct gccagcgccg gtgaggcgca aggagcgctg ggtcttctgg
     8581 cgcgtatgca tccggtattc cccgcataag cggcggtttt ccttgactca ccgggaaaac
     8641 atcccgagaa tcccggcata ccgaaatacg gaacccggct ggcccaagta gcgtctttgg
     8701 gcgtccgaag cttagggatc ccgtacgtag ggaaaagaat ggtggaaatg tcgtctgccc
     8761 gccaattctg ttcaataatc ccgcggcgac atggcaatac cttgggaaac aattctggag
     8821 aggaatttca atatgactag cactaaacgc tacctgcgca tcgcgctggc ggcggcgttc
     8881 tccgcggcag ttgccgccgg aagttcgctc gcagctgatg tcgatggcgc ccgcgtgcaa
     8941 aatgcggaca aggaacttga aaaaggcaac tggctcacgt accacggctc gtacaaatcg
     9001 tggcactaca gcccgctcga tcagatcaat accaaaaccg ttggcaaact ttccgaagcc
     9061 tggtcacaca cagcatcacg cgccaatcgc ggattgcagg gctttccgct cgccatcgac
     9121 ggcatactgt attactcgag cccctacaac caggtttacg ctctggacgg cgcaaccggc
     9181 cagatgctgt ggacatataa acaaaagttg aatgaagacc tggtggcccg gcaaacgcac
     9241 tcaccgtaca accgcggtat tgcagccggg tacggcaaca tctttatcgg cacgctcgat
     9301 ggcaagctgg ccgccatcga catgaaaacc ggcaagctga gctgggaaac caagctgatc
     9361 gattctgaga agctgacagt cggctttacc ggcgcaccgc tgctcgtaaa agataaagtc
     9421 atcatcggtt cgcaaggcgg cgagtggccg taccgtggtc caatcttcgg ggtcaatgcc
     9481 aagaccgggg aaaaggtctg gcaattcttc accgttggcg gcaatgacgg ctctgcgagc
     9541 gacaagcgcg acacatgggg caatgattcc tggaaagttg gcggcggcgg tggctggatg
     9601 gccggcgcct atgatccgga aacaaactcg gtatggtggg gtaccgccaa cccggccccc
     9661 ttgtacgatt ggtcgggcga caagtggatg acagagggtg cgcgtcctgg catcaacttg
     9721 tacatcacct cggtgatcct gcttgatccg gacactggtg acctgaagtc ctatcatcag
     9781 gaactgccgc acgatgcatg ggatttcgac tcgtccatgg gtgagttcgt gttcctggaa
     9841 cgcgacggca aaaagtatgt cgtgcatcca aacaaaggtg gtttcgtatt tgtttatgac
     9901 cgtaacggca agcccgtcaa tgtttacaaa ggcgtcgatg ccatcacgtt cgtgaaagac
     9961 atcaagcccg acggcacgct ggtcggacgt caggactaca ccgaaggcaa gcacgaaaac
    10021 ctgtgcccgg caattgctgg tggctacagc tggaatgcgg gaacctacaa tccgaagacc
    10081 ggcctgttct accgcgttgg ctacgagtgg tgcatcgact tcacggtcgt gaagaccgag
    10141 ccgatcaccg agccggtagt gcagttgaac atcggtgcgg atttcacatt cgtgccgcct
    10201 aaaggcaaga aatcgatggg tggccacatc cgcgcccgcg atccgatcag cggcaaggtc
    10261 aagtttgaaa ttccgtacgt cggggcaacc ccgcatgcca gcctgttgag cacgggcgga
    10321 aatgtgctgt tcgttcctga agccgacgga acattggctg cgtttgatgc aaccaatggc
    10381 aagaaactct gggcgcacaa cgatggccag ggccacaacg gcggcatcat tacttatctg
    10441 gccaagggca agcagtacgt tgctgtcatg accggatggg gttcgctggt gggcgacggc
    10501 tacggcgact ggtacggcga gccgtggaag agcatgccaa aagattccgg cgtgctgaaa
    10561 gtgttcgctt tgcagtaatc cggcaagcag ctttcataag ggcgggaacc tgtgttcccg
    10621 ccctttttta tgacgtttgt ccaccacccg atactcatgg cgttatgtcg tataacaggc
    10681 gcgaactctg tgccaaagat tcatgtctat caaaagttcg atgtttgtcc agaggatgac
    10741 ggttgtgaca actacaaaga cttacttgca agtgcgatgc ctgctgctaa ccacagtact
    10801 tggttttgcg ttgtgcttca cggcacaagc tcaagaacag aaggctgccg caaaacatct
    10861 ggatgtcaaa agcacgtttc gcaacatctg tggattctgt cacgagaatt atggtcgcaa
    10921 ggccgccaag gggccgcagt tgatgaactc ggaacggtcc gacgaattca tctttaaccg
    10981 gatcaaaaat ggcatgccgg gccgcatggc agcattcggg acggctttca cggatgatca
    11041 gatccgcgac atcataaaat tcatacgtag tcttaagccg gacgaggagc cgcaaaatcc
    11101 atcatgacct tcaacttgcc acagcgcgca ggatatgcat ttgtctgcgt cgcgttgctg
    11161 ctcatcgcag ccaatacagc cggagcccgt acgcttgctg agatcaagtc gttgggtggc
    11221 attagcatgt gcgccaacag agacgcgctg ccatacgcca gcaataaacc cgagacaccg
    11281 gggtttcaga tcgaaatcgg gcgcgcgatt gcggaaggcc tgggcgttcc cctgagcatc
    11341 gaatggattc ttccgcgtcg gcgcgcaaat gtcgtcaact gcgacatgct actggacaat
    11401 atcaacgacg ctgaagtgaa cgaggggcgc atgcgtttgt cgcgtcctta ccagaaatcc
    11461 ggtttggcgc tcgggctgcg gaatgatagc gagcttgtca acgacttcac agaattgaag
    11521 aagggccaga agatcggcgt aatgatcaac tcgtatgcgg ccatggtgct ggggaaggcg
    11581 ggcaagacgt tctcgccgta tgcctttcag tccgacatgg tggaagattt gcagaagggt
    11641 gagttgtatg gagctgctgt ttcagcggcc accatgagct attacattcg ccaacacccg
    11701 gagtccggat tgcggttggt gcacgcgttc gatagcgtgc cgcaactgac ttgggaagtg
    11761 tcggtggggc tgcgcaaggc ggatgctgcg ctggtggacg cggtcaacga aatactggac
    11821 aagttgatag cggatggcac gctgacaagc atctacgaga aatacggcgt ggagcatcgt
    11881 attccgtaga cggtcggtaa tcaggaggcg gaagccttaa ccttgcgctc tgtgagcgac
    11941 agatatttct ggtacagctg ttcctggctt tcaacgcaat cgggattcag cggtatgcag
    12001 tccacagggc agacttcctg acattgcggc ctgtcgtagt gcccaacgca ttccgtgcat
    12061 ttgtcggggt cgatctgata aatgtccacg ccttgtgaga tggcgttatt gggacattca
    12121 ggctcgcata catcacagtt gatgcattcg tcagtgatca gcaaagccat gcttatccgc
    12181 gctcgagtct ggctttcatt cgtggaatca ccagtggatt gacgaagggc gagacgtctc
    12241 ctcccagcga gacgatttcg cgcaccatgg tggcagagat gaacatgtac tgctcggcag
    12301 gagtcaagaa caacgtttcc acatcagggt atagcttgcg attcatgcct gccatctgga
    12361 actcgtactc gaaatcggat accgcccgca gtccacgcag aattattttc gcatcatgct
    12421 tttgcaagaa ctgcatgagc aaacctgaaa atccttccac gcgaacattc cggtacgccg
    12481 ccagtgcttc cttcgccatg gcgacgcgtt catccacgtc aaagaacggg cgctttcccc
    12541 tgctatcggc aaccgccacc agaacctcat cgaataaatt cgacgcacgc cggaccagat
    12601 cctcgtgtcc gcgggtgatc ggatcaaacg taccgggata gacggcccgg ttaatgccat
    12661 ttttcatgat gaacggcatt ttatcagcag accatgcacc tgcccgacac gggtttgttt
    12721 gagcaaatca aacgcgtcgg gcacgccgat aggctcttct gcctccgcat atacacacgc
    12781 gtgcgccgcc aaatgtcctg gcagctctgc catcactcgc ggcatcaatc cgtgccggaa
    12841 tggcgggtcc aggaatacca cgtcataacg ccgcacatca gtcttgagga aggaaaaggt
    12901 atccgcgtga tagagttcga cctgctccgc accaagtaac gcccgggttt tccgcagggc
    12961 atgataaaca ccggtattgt gctcgaccat tacaaccggt cgtgcgccgc gcgatgcggc
    13021 ttcgaagcca agagccccgc ttcccgcaaa caggtccagg cagcttttcc cggtgaggtc
    13081 ctggcccagc cagttgaata gcgtttcccg caaccggtct ggcgtcggcc gcaaggattg
    13141 ggcctctgga aaacggacca ggcggctgcg ccactgcccg ccgattatgc gcacttgatt
    13201 cgtcttcaag ccggaaccgg ttcattcaag tccagcgaag gctatcacgc cttggcgttg
    13261 aaaacagctc ttcgatatag gaggtcagtg tgcctcggta cccgcgacga tcaccgtcac
    13321 catgcgatcg ggatagatac gccggcgcca ggcatccttg acctgttcaa gtgtcagctt
    13381 ttcgatcttg accggaaaat cgtccagata agttagcggc agacgataaa agccgatgat
    13441 tgccaggtaa tcctggatct tcttgttgct gtcgatgcgc agagcaaacc cgccaatgat
    13501 gttctgtttc gccgcatcca attcttgctg tgttgggcct tctgccacaa agttggcgag
    13561 ggtgtcctga gcaaccttca aagcctgttc agcctgctcg cttttggtct gcagcgcgat
    13621 ctggaaagga ccttccaggg caagcggatt gaaatagctg taggcgctgt atgcgtatcc
    13681 gcgtttttgg cgaatctcct cattgagccg ggaagcaaaa ccgccgccac cgagcacctg
    13741 gttgcccaga aatagcgcaa agtaatcggg atccagacgt ttgatgccgg gttggccgat
    13801 caaaatatgc gcttgcgttg ccggatgcgg gattcgacta agctgggccg acttcagttg
    13861 ctgaactggc ggcagaagcg ccactcgctg cgactttgac ggcaggccaa cggtcaggag
    13921 ctctgcaata gcgctggctt ccttgcgcgt aagatcgcca atgatcgata ccgttgcatg
    13981 gtcggcaaca taatgctgac gatagaaatt gaccacgtcg tcgcgctgca actttgcaac
    14041 ggtattcaac tcacccgccc cgcgctgaga ataagggtgc tggccgtaca ccaggtgctt
    14101 gaattcacgc tccgccaaca cttcgggcct ggactccgcc tccttcagcg cggctagcgt
    14161 acgctgctgc tcccgttcta aaattggctg cggaaaggtt ggtttctgca acaccgttgc
    14221 taatattttc agactttcgt cgcgctcgcg agcgctggac aaactgcgca atccgtaccc
    14281 cgcccggtcc aaatcgaacc gctcggacat atcagcgcca atgctagcca ggcgttctgc
    14341 aatctcaccc tcgctcatgt cacccgcacc gctccgcatt aattcaagcg taagcgacgc
    14401 taagccggac ttatcgcgca ggtcacgact tgcaccggcc gggaaatcaa ccgccaggtc
    14461 aaacatcggc aaatcgtgcg tttcgacaaa tagcacccgg gccccgtttg ctgtctgcca
    14521 ttcctggatc ggcaaagtcg catgcgctgt acatgccgca gccaggcaaa ggcccgcaag
    14581 acctttgaaa aggtattgac tagcgctggt cacgtgcgcc tccttccgga ttcggccttg
    14641 gacgctttcc ggcaggcagg ggttgcggat cgagtaccgc gacggtgaga ttgtcgtcga
    14701 ccagatactt ccgtgcgacg tcgcgtactt gctctgcggt aacttgctgc agcttcctga
    14761 cctggagatc cgaggagtca taaggcaaac cgatggtctg cagcatcccg atgcgcatgg
    14821 cctggtaata aaccgaatcg cgtgcgtaaa cctgggccgc agttacttgc gctttgacgc
    14881 gcattaattc gtcattactg acaccctcgg tgactaaaag ccggatttgt tcgcgcaacg
    14941 ccgcctcaac gtcctgtacc gtcttgcctg ccgctggaac gccatccaga aagaacaatg
    15001 cgggtccgcg attgagcatg tcatagttgg cattggcaga atccgcaacg cgcatttttc
    15061 gtacgagctc gcgattgagg cgcgatgcat cgcttccgtc aagaataccg ctcagcacag
    15121 cgagcgcata aggttcccag tcatgctcaa catcacgcaa tgacggcgcg tggtacgcca
    15181 tggcgacata aggaagttcc gcctgtgcct ttacgatgat tcgctttatg ccggcctggg
    15241 gaggctccgt ttgaaccttg cgcgctggcg gcgtacgtga tggtaaagca ccaaaatatt
    15301 tctctcccag cttgaacacg tcgtccggtt tgacatctcc agcaacaact aacgtggcgt
    15361 tatttggcgt gtaccacttc tcgtaccacg cgcgtgcgtc agcaagctgc atgttctgca
    15421 ggtcgttcat ccagccaatg atgggagtac gataaggatg cgccatgtag accgtcgcca
    15481 tcatttgctc gtgcacctgg gcatgtggat tatcatcggt acgcgaacgg cgctcttcca
    15541 tcaccacgtt catctctctg gcgaactcgt cctgcgcgaa gtttagattc gacatccgat
    15601 cggcttccag ctcaagggca agggatagtt tggaattgtg caattgctgg taatacgcgg
    15661 tgtaatcgtg agatgtgaag gcattctcgc gacctcccgc gcgcgcaatg gtcctggaaa
    15721 attcgcccgc tggcacactc ttggtacctt tgaacatcat gtgttcgagc atatgggcga
    15781 ca
//
LOCUS       DATMZQ010000100         5906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1118345, whole genome shotgun sequence.
ACCESSION   DATMZQ010000100 DATMZQ010000000
VERSION     DATMZQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118345"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VN496_03125"
     CDS             <1..279
                     /locus_tag="VN496_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXF15966.1"
                     /translation="PVRGRILISTGLALHECALAGMGLALLPHWVIGKDLHHGRLVDV
                     FPEFEVTPTTFNTAAWFVYPSTTRPPKKVRLFVDYLQKEIRDNPPWMR"
     gene            332..1072
                     /locus_tag="VN496_03130"
     CDS             332..1072
                     /locus_tag="VN496_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15967.1"
                     /translation="MTVSESSKGVCLVVGAGDATGGAIARRFAREGFTACVTRRSADK
                     LATLVEQIRSAGGKVHAFGSDARKKDDVVDLVEEIETTIGHIEVLVFNIGANAASSIL
                     EETARKYFKIWEMGCFAGFLNGREVARRMVVRQRGTIIFTGATASLRGSANFAAFAGA
                     KHALRALAQSMARELGPMGIHVAHTIIDGAIDTAFIRDLFPDRYALKEQDGILNPDHI
                     ADAYWMLHTQPRDAWTHELDLRPYLEKF"
     gene            1069..1680
                     /locus_tag="VN496_03135"
     CDS             1069..1680
                     /locus_tag="VN496_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXF15968.1"
                     /translation="MMKSFEFWFDFGSTASYLAWTQLPALETATKSKAVLRPMLLGGV
                     FQATGNQSPANIPAKGKYMFVDFERFAKRYGVQFNRNPNFPINTLLFMRGAIALQSKN
                     DPKFLDYCHAIFNAIWVQSLNMNDPATAAKGLQNAGFDAQTLVALANEPATKDALKAA
                     TQTAVERGVFGAPTFFVGDEMFWGQDRLDFVKEALQKQAVSLP"
     gene            1677..2729
                     /locus_tag="VN496_03140"
     CDS             1677..2729
                     /locus_tag="VN496_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF15969.1"
                     /translation="MTKRTALVTGASGLVGSYLLKHLLDQGDWDVVAISRRKPDIPGN
                     YKHIAVDLLNPADCKSRLGGLTQVSHLFFVAYIERSDAQQLVFDNTAMLVNVVDVIEA
                     ASPGLQHVHLSEGSKWYGNHLGPYKTPSKEDDPRHMPPNFYYNQQDFLEARQKGKRWS
                     WSTVRPHAVCGFSTGGPMNLTLSIAVYASISKALGLPLSFPGKPGAYSALYQCTDAGL
                     LAKSIVWMATEPKCANQAFNITNGDLIRWQYVWPKLAEFFQMGVAPLRTISLTRTMAD
                     KGPVWDRIVADHKLKPHRYQDIVSWGYPESVFNSDYDIISNTGKARRFGFHDSLDTED
                     MFLRMFAEFRSNRIIP"
     gene            complement(2787..5717)
                     /locus_tag="VN496_03145"
     CDS             complement(2787..5717)
                     /locus_tag="VN496_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXF15970.1"
                     /translation="MAARVRAFDWATTALGPVEGWPQSLRIAVGICLHSRFPMFVWWG
                     PQLIKIYNDSYVPMLGTRHPAALGRPAQETWSDIWDVIGPQAEAVMKRGEATWNERVL
                     LMMERQGYSEETYFTWSYSPIYDDSGGIGGVFCACVEETSRVLTERERDRLLKDNDAE
                     RLRLAAAFAQSPSFLAILRGPKHVFEYVNERYGRLIGKREVVGRPVREAIPEVEGQGF
                     FEILDRVYATGEPFVGNAMCAYIRRHEHRPPEETFVDFIYQPMRGSTGEVAGILVHGM
                     DVTERKKAEARDRFMLALEDALRPLTEPAQITATGVRLLGEYLQANRCAYADVEQDED
                     TFNLMGDYNNGVPSIVGRYRFSDFGAEVLALMRTGRPYLVRDVDAHQPPIGDLSYYRK
                     TLIRAVICVPLLKAGKFVAAMAVHQATPRDWSDADAELLSHVASRCWESIERARVERT
                     LRESEADFRELADAMPQIVFSAGPDGDVDYFNRQWYEYTGLAEGQIGYDSWKPVHTED
                     GLRRALEAWPRAVRTGEPYEIEYALRRHDGEYRWHLGRALPIRDENGKIVRWFGTNTD
                     IHDRKQTEQALATSLDAEQRARSEAELASRMKDEFLANLSHELRTPLTAILGWSHILR
                     RDNVTPQQLANGADVIERNARAQAQIIEDLLDMSAIISGKVRLQIAENVDLRAVVARA
                     IETTRTAADAKRIAIRVDVGAGDRLNINADASRLQQVIWNLLSNAIKFTPAGGRVNVN
                     AVRLQDVVEISVADTGEGIGPHFLPYVFDRFRQADASTTRRHGGLGIGLSIVKQLVEL
                     HGGMVRVFSEGEGRGSTFSVTLPAESRFAQTDTSAGKRRGTDLDSPAKDSDRIRGARI
                     LVVDDDADARDLLRRLLEDCGAQVALSGSSADAIEIIRQGQLDLVVSDIGMPGEDGYA
                     LMRRVRMLPAEEGGKVPALALTAYARAEDRVKAIRAGYQMHVPKPVDPAELIAVVASM
                     STSK"
BASE COUNT         1167 a         1810 c         1716 g         1213 t
ORIGIN      
        1 cccgtccggg gccgcatcct catttcaacc ggccttgcgt tgcacgaatg cgcgctcgca
       61 ggaatggggc tggcgctgct gccgcactgg gtcatcggca aggaccttca tcatggccgg
      121 ctagtggacg tgtttccaga gttcgaagta actcccacga ctttcaatac tgctgcctgg
      181 ttcgtttatc cgtccacgac gcgtccgccg aagaaagtgc gtctttttgt cgactatctc
      241 cagaaagaaa tacgcgataa cccgccatgg atgcggtagg agcacgcatc ctcgattaga
      301 ctttgcctca caagaacgga accaataaat catgactgtt tccgaatcga gcaaaggggt
      361 atgcctcgtt gtcggcgcgg gtgacgcgac gggcggcgcc attgcgcggc gtttcgcccg
      421 ggagggattt acggcgtgcg tgacgcgccg ttccgctgac aaactggcga cgctggtaga
      481 gcagatacgg tctgccggcg gcaaagtcca tgctttcggc agcgatgccc gcaagaaaga
      541 tgacgtggtt gacctggtgg aggagattga aaccaccatt ggacacatcg aagtgctggt
      601 attcaatatt ggcgcgaacg cggcctccag catcctcgag gaaacggcgc gcaaatattt
      661 caagatctgg gaaatgggtt gtttcgccgg tttcctgaac ggtcgcgaag ttgcgaggcg
      721 catggtggtg cgccagcgcg gcacgataat tttcaccggg gccacggcct ctctgcgcgg
      781 ttccgccaac tttgccgcgt ttgccggcgc caaacatgcg ctgcgcgcgc tcgcacagag
      841 catggcgcgc gagctcgggc ccatgggtat ccacgttgcg cacacgatta tcgacggggc
      901 cattgacacc gcatttatcc gcgatctttt cccggaccgc tatgccctca aggaacagga
      961 tggcattctc aaccctgacc atatcgccga cgcgtactgg atgttgcaca cccagccacg
     1021 cgatgcctgg acgcatgaac tggatctgcg gccctatctg gagaagttct gatgaaaagc
     1081 ttcgagttct ggttcgattt cggcagcacc gcttcttatc tcgcgtggac tcagcttccc
     1141 gctctcgaaa cggcaacgaa atccaaagcg gtcttgaggc cgatgcttct gggcggcgtg
     1201 ttccaggcca cgggcaatca gtcgccggcc aatattcctg cgaagggaaa gtacatgttc
     1261 gtggacttcg agcggttcgc caaacgctac ggcgtgcagt tcaaccggaa cccgaatttt
     1321 ccgatcaaca cgctgctgtt catgcgcggc gcgattgcat tgcagtcgaa gaatgacccg
     1381 aaattccttg actactgtca cgccatattc aatgccattt gggtacagtc gctcaacatg
     1441 aatgaccccg ctacggctgc caaaggcttg caaaatgcgg gcttcgatgc ccagacgctg
     1501 gttgcgttgg ccaatgaacc cgcgaccaag gatgcgctca aggcggcgac acagacagcc
     1561 gtggaacgcg gcgtgttcgg tgctccgacg ttttttgttg gcgatgagat gttctggggt
     1621 caggaccggc tggactttgt gaaagaggcg ctgcaaaaac aagcggtgtc cttgccatga
     1681 ccaaacgcac cgcactggtt accggagcct ccggcctggt cggaagctat ttgctgaaac
     1741 atttgcttga tcagggtgac tgggatgtcg tggcgatttc tcgccgcaag cctgacatac
     1801 cggggaacta taagcatatt gcggtggacc tgttgaatcc ggccgattgc aaaagcaggc
     1861 tcggcggact gacgcaggtt tcgcatctct tcttcgtagc ttatatcgag cggtccgatg
     1921 cgcagcagct ggttttcgac aacactgcga tgctcgtcaa tgtggtcgat gtcatcgagg
     1981 cggcgtcccc ggggcttcag catgtgcatc tttccgaagg cagcaagtgg tatggcaatc
     2041 acctcggtcc gtacaaaacg ccatcgaagg aagatgaccc gcgccacatg ccgcccaatt
     2101 tttattacaa ccagcaggat ttcctggaag cgcgccaaaa gggcaagcgc tggagctggt
     2161 ccacggtgcg tccgcacgcg gtgtgcggat tctcgaccgg tggtccaatg aatctgactt
     2221 tatcaatcgc cgtttatgcg agcatttcca aagcgctggg actaccgcta agttttcccg
     2281 gcaagccggg ggcgtacagc gcgctgtatc aatgcacgga tgctgggttg ctggcgaagt
     2341 ccatcgtatg gatggccacc gagcccaagt gcgcgaacca ggcattcaac atcaccaatg
     2401 gcgatctgat ccgctggcaa tatgtgtggc cgaagcttgc ggagtttttt caaatgggcg
     2461 tggcgccatt gcgaaccatc agtctgacac gcaccatggc cgataaaggg ccggtctggg
     2521 atcgcatcgt ggccgaccac aagctcaagc cgcaccgcta ccaggacatc gtctcgtggg
     2581 gctatccgga gtccgtattc aattcggact acgacatcat ttcgaacacg ggcaaggcac
     2641 gccgcttcgg cttccatgat tcattggaca ccgaggacat gttcctgcgc atgtttgcgg
     2701 aattcaggag caaccgcatt attccgtagg ccccattggc taccattgca acaaggcctg
     2761 acgaaattga agaacgggct ggcaaattac tttgaagtgc tcatgctcgc caccaccgcg
     2821 atcagctctg ccgggtcgac gggtttcggc acgtgcattt ggtagcccgc gcggatcgcc
     2881 ttgacccggt cttccgcgcg cgcataagcc gttagggcga gagcagggac tttcccccct
     2941 tcttcggcgg gcagcatgcg cacgcggcgc atcagggcgt agccatcctc gcccggcata
     3001 ccgatatcgc ttacgacaag atcaagttgg ccttgccgga tgatttcaat tgcgtccgca
     3061 ctggacccgc ttagcgcaac ctgcgcgcca caatcctcca gcagacgcct caacaggtcg
     3121 cgcgcatccg catcatcgtc aaccaccaga atgcgcgcgc cacgaatgcg gtccgaatct
     3181 tttgcaggcg aatcgaggtc tgttccacgc ctcttgccgg cactcgtgtc cgtttgcgcg
     3241 aaccgggatt cggctggcaa cgttaccgag aaagtcgagc ctcggccctc gccttcacta
     3301 aacacgcgaa ccatgccgcc gtggagctcg acgagttgct tgacgatgga caggccgatc
     3361 ccgagcccgc cgtggcggcg cgtggtcgag gcgtcggcct ggcgaaaacg atcgaacacg
     3421 tagggcagga aatgcgggcc gatgccttca ccggtatccg ccaccgagat ctcgacgaca
     3481 tcctgcaggc gcacggcatt cacgttcaca cgtccgcccg ccggcgtgaa tttgatcgca
     3541 ttgctaagca aattccagat tacctgctgc aggcggctgg cgtccgcatt gatgttgagt
     3601 cgatcgccgg ctccgacgtc caccctgatg gcaatacgtt ttgcatcggc agcggtgcgc
     3661 gtggtctcga tcgctcgggc cacgacggcc cggagatcga cgttttctgc aatctggagg
     3721 cggaccttgc cggagatgat ggcgctcatg tcgagcagat cctcgatgat ctgcgcctgc
     3781 gctcgcgcgt tgcgctcgat gacatctgca ccgttggcca gttgctgcgg cgtgacgttg
     3841 tcgcggcgca gaatgtgcga ccatccaagg atggccgtca acggagtgcg cagttcatgg
     3901 ctcagattgg cgaggaactc gtctttcatg cggctcgcca attcggcttc cgaacgagcg
     3961 cgctgctcgg catcgagcga tgtggcgagg gcctgctccg tctgcttccg gtcatggatg
     4021 tcggtattcg tgccgaacca gcgcacgatc ttgccgttct cgtcacgaat cggcagcgca
     4081 cggccgagat gccagcgata ttcgccatcg tggcggcgca gcgcatattc gatttcgtat
     4141 ggctcacccg tgcgcaccgc gcgtggccac gcttccagcg cgcgccgcag cccatcctcg
     4201 gtatgtaccg gcttccagct gtcgtagccg atctggccct cggcaaggcc ggtatactcg
     4261 taccattgcc ggttgaagta atcgacatcg ccatccggcc cggcggagaa cacgatctgc
     4321 ggcatggcat ccgccagttc ccggaaatcc gcctcgctct cgcgcagcgt gcgctccacg
     4381 cgtgcccgct cgatcgattc ccagcagcgg cttgccacat gcgacagaag ctcggcgtcc
     4441 gcatcgctcc agtctcgcgg ggtcgcctgg tgcaccgcca tcgcggcgac aaacttcccc
     4501 gccttcagaa gaggcacgca aatgactgcc cggatcagcg tcttgcggta gtacgaaagg
     4561 tcgccgatgg gcggctggtg cgcatcgacg tcccgcacca ggtaaggccg gccggtgcgc
     4621 atgagcgcca gcacttcagc gccaaaatcg gaaaagcggt aacgcccaac aatactcggc
     4681 acgccgttgt tgtagtcacc catcaaattg aacgtgtctt cgtcttgttc aacgtcggca
     4741 tatgcgcatc ggtttgcttg aagatactct ccaagcagcc gtacccccgt tgcggtgatc
     4801 tgcgccggtt ccgtcaacgg acgcaacgca tcttccagtg cgagcatgaa gcggtcgcgc
     4861 gcttccgcct tttttcgctc ggtaacgtcc atgccgtgga ccagtatgcc ggccacctct
     4921 cctgtgctgc cgcgcatcgg ctggtagatg aaatcgacaa acgtctcttc cggcggacga
     4981 tgctcgtggc gtcggatata agcacacatg gcattaccaa cgaacggctc gccagtggca
     5041 taaacgcggt cgagaatttc aaagaaaccc tgcccttcca cttccggaat ggcctcccgg
     5101 accggcctgc ccaccacttc gcgcttaccg atcagtcggc cgtagcgctc gttgacgtac
     5161 tcgaacacat gtttcggccc gcgcaggatg gcgagaaaag acggcgactg cgcaaacgct
     5221 gctgcgagac gcaggcgctc cgcatcattg tctttcaaca ggcggtcgcg ctcgcgttcg
     5281 gtcaacacgc gtgacgtctc ctcgacgcag gcgcaaaaca cgccgccgat gccacctgaa
     5341 tcgtcgtaga tcgggctgta agaccacgta aagtacgtct cttcgctgta gccctggcgc
     5401 tccatcatca gcaggacacg ctcgttccag gtggcctcgc cgcgcttcat cacggcctcc
     5461 gcctgcggac cgatcacgtc ccagatatcg ctccacgttt cttgcgccgg gcggcccaat
     5521 gcggcagggt ggcgtgtgcc cagcatggga acgtagctat cgttatagat tttgattagt
     5581 tgcggccccc accagacaaa catcgggaac cgcgagtgca ggcagatgcc cacagcaata
     5641 cgcaagcttt gcggccatcc ttcgaccggc ccaagcgcgg tggtggccca gtcaaaagcc
     5701 cggacccgtg cggccatttc gccgcctgct gcattcagtt cagacaaaat catccgggct
     5761 atggccaacc tgctgtggga aagttcagaa agcgcaggtt tcgtggcatc tcacgtcctg
     5821 agcgacagcg ccgtgacacg gcgagtcgct ttcttctagt gtggtatgcg cggttccatc
     5881 gtccgagctg tgcgctaacc atcacg
//
LOCUS       DATMZQ010000101         4311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2499136, whole genome shotgun sequence.
ACCESSION   DATMZQ010000101 DATMZQ010000000
VERSION     DATMZQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499136"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /gene="pip"
                     /locus_tag="VN496_03150"
     CDS             <1..1048
                     /gene="pip"
                     /locus_tag="VN496_03150"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HXF15971.1"
                     /translation="FATNAARGPAYRTRSHQIQAGFLTSAKSAMAVEQNEAIADVLYP
                     PLAPYRNGLLDVGGGHQIYFEESGNPAGFPVLFVHGGPGSQTRPAHRRFFDPQFFRIV
                     LFDQRGCGQSKPQALLADNTTSHLVADMERLRGHLNINRWLLFGGSWGSTLSLLYAIA
                     YPDPVAGLVLRGVFLASCAEVDWFLYGVRHFVPEAWSRFTQQSTPGIVAHYQRLVHNA
                     QPEIASAAAQNWSDYEARVMDPANTAASAGSSSGRDNLAAVQVHLHFLSKQFFLKPNE
                     LSDNLWRVKDMPVTIVQGRMDMVCPPMTAVAVALGIKGSELRLVANGSHSAFQREIAI
                     ELCAATRDMKAKFG"
     gene            1055..2812
                     /locus_tag="VN496_03155"
     CDS             1055..2812
                     /locus_tag="VN496_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXF15972.1"
                     /translation="MHTLSLRFRINLVITLVVVAFTVATGNLLIEETRRSVREEMEAG
                     TRITAQLLETVIANSRLNFQQEVHNQMFLSFLQRVGRVRANEIRLYDESDRLVYTSPP
                     PIYKRGRWAPEWFSRLVSPKLSDYRLNLPTGSVVITPDPSRAVLDAWDDLKQFFWLVM
                     GFFILVNVVVFWLLGRSLRPIGKILGGLSLMEKGRFETRLSLFGSPEFDSIGHVFNRM
                     AQTLEESLAENRRLALVTRQSSDAIMIHDLEGRISFWNPAAERMFGYRMDEIAGRSAT
                     LLTPLERHDEIQENLNTVKRRELIELRETQRLTKDGRVVDVALSAAPLVDPASDQVIG
                     EICSMRDITEHKLVQLAERELEQNRQLTRAIQSRLEEERRSIARELHDELGQCVTAIK
                     TIGTAIANRAQDSEPDTHANALTIVSVASHIYDVVHGLIRQLRPTALDHLGLSDTLRD
                     TVAAWRERHPDITCDLRLEGKLDGMGETLNITVYRTVQECLTNIARHAAASRAVITVT
                     RQMGNREVDDAVRVTVSDNGKGFGEQPYRDVMRFGLIGMRERVQALHGEFRIESTPGQ
                     GATVTVLIPVAAAAGGPQG"
     gene            2817..3470
                     /locus_tag="VN496_03160"
     CDS             2817..3470
                     /locus_tag="VN496_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF15973.1"
                     /translation="MEGKSIGNISVMLVDDHAVVRMGFRLLLEGSADIKVVAESDSGE
                     DACRKFPDVKPDVVVMDISMPGIGGLEAIDRILARNPGARILVLSAHEDAMHARRVLK
                     AGAAGYLTKRSAAEALIQAIRQVHQGKTFLEPEIAQQLAMQQLNGERNPLDMLSEKEF
                     KVFLALAKGQSVADIADVMSLSPRTIGTHLYNIKQKLGASNSAELAIIAIRAGLINP"
     gene            3643..3714
                     /gene="pqqA"
                     /locus_tag="VN496_03165"
     CDS             3643..3714
                     /gene="pqqA"
                     /locus_tag="VN496_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HXF15974.1"
                     /translation="MWTKPEYTEMRFGFEVTMYVFNR"
     gene            3917..>4311
                     /locus_tag="VN496_03170"
     CDS             3917..>4311
                     /locus_tag="VN496_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HXF15975.1"
                     /translation="MNRNALMLAATAMTMLSVSVLAEPFAYVPNEESGTISVIDVSTD
                     EVVKEIRAGDKPRGMAASKDGKKLYVSDQPHNSLVVIDLQKLEPVDSIFLGESPEGVG
                     ISPDGKWVVAAVEVSNAIAFINTESNKKE"
BASE COUNT          983 a         1093 c         1247 g          988 t
ORIGIN      
        1 cttcgctacc aacgccgccc gagggcctgc gtaccgtact cgttcccatc aaattcaggc
       61 tggattcctg acgtccgcca agtcagccat ggccgtagaa cagaatgagg ccatcgcaga
      121 tgtcttgtat cccccgctgg cgccttatcg caatggcctg ctcgacgtgg gtggcggaca
      181 tcaaatatat tttgaggaaa gcggaaatcc ggctggtttt ccggtgctgt ttgttcacgg
      241 cggacccggc agccagactc gtcctgcgca tcgcagattt tttgacccgc aattttttcg
      301 catcgtactg ttcgaccagc gcggttgcgg ccaaagtaaa cctcaagccc tgctagccga
      361 caacacgacc tcccatctgg tagcggatat ggagcgcctc cgcggccatc tgaatatcaa
      421 tcgctggtta ctgtttggcg gatcctgggg gagtacgcta agcctcctgt acgccatcgc
      481 gtatcccgac cccgttgcgg gattagtcct gcgcggtgtc ttcctcgcca gttgtgccga
      541 agtggattgg ttcctgtatg gcgtacggca ctttgttcct gaagcatggt cgcggttcac
      601 gcaacagtcg acccccggca tcgtggcaca ctatcagcga ctggtgcaca acgcacagcc
      661 cgaaattgca tcggcggcgg cacagaattg gtctgactac gaggcgcgag tcatggatcc
      721 ggcaaataca gcagcgtctg cgggtagttc ctcgggccgc gataatctgg cggcggtcca
      781 ggtccatttg cattttcttt caaagcaatt ttttctgaaa ccaaacgaat tgtcagacaa
      841 tctttggcga gttaaagata tgcctgtcac gattgtgcag ggcaggatgg acatggtttg
      901 cccgccgatg acggccgtcg ctgttgccct tggcataaag ggatcggagc tacgcctggt
      961 cgcgaatgga tcgcactcgg cgtttcagcg cgagatagcc attgagctgt gcgctgctac
     1021 ccgggacatg aaggcgaagt tcgggtaaac gccagtgcat acgctaagcc ttcgttttcg
     1081 aatcaatctc gtaattacgc ttgtggtcgt cgcgtttacg gttgctacgg gcaacctgct
     1141 gatcgaagaa accagacgct ctgttcgtga ggaaatggag gccggaacaa gaattaccgc
     1201 gcaattgctg gaaacggtga tcgccaattc ccgccttaac tttcaacagg aagtgcacaa
     1261 tcaaatgttc ttgtccttct tgcaacgagt gggccgggtc agggcaaacg agatccggct
     1321 ctatgacgaa agcgatcgtc ttgtgtatac ctcacccccg ccaatctaca agcgtgggcg
     1381 ctgggctccc gaatggtttt ctcgactggt cagtcccaag ttaagcgatt accgtctgaa
     1441 tctaccaact ggcagcgtgg ttattacacc ggatccttcg cgtgctgtcc tggatgcgtg
     1501 ggatgatctg aaacaatttt tctggctggt aatgggcttt ttcatcctgg ttaacgttgt
     1561 tgtgttctgg ttgctggggc gttcgctgcg tccgatcgga aaaatcctgg gcggactgtc
     1621 attgatggaa aagggacgtt ttgaaacgcg tctatcccta tttggctcgc cagagtttga
     1681 ttcaatcggc catgtgttca atcgcatggc gcaaacgctg gaggagagcc tcgccgaaaa
     1741 caggcgtctg gcgctggtga ccaggcaatc cagcgatgca atcatgattc atgacctgga
     1801 agggcgaatc tcgttctgga accctgccgc cgaacgaatg tttggttacc gcatggatga
     1861 aattgctggc cgttcggcga cgcttttgac gccgctggag cggcatgacg agatccagga
     1921 aaaccttaat accgttaagc gacgcgagtt gatcgaattg cgcgagaccc agcgcttgac
     1981 caaagacggg cgcgtggttg acgtcgcgct atccgctgcc ccgctggtcg atccggcaag
     2041 cgatcaggtt attggcgaga tctgcagcat gcgtgatatc acggaacata agctggttca
     2101 gcttgccgag cgtgaattgg agcagaatcg gcaattgacg cgcgcaatac agagccgtct
     2161 ggaggaggag cgccgaagta ttgcacgcga gttgcatgat gaactggggc agtgtgttac
     2221 ggcgattaaa accattggaa cggcgatagc gaatcgcgcg caagacagcg aaccggacac
     2281 tcatgccaat gcgctaacaa tcgtatcagt tgcctcgcac atttatgatg tggtgcacgg
     2341 cctgattcgg caacttcggc ctaccgcgct cgatcacttg ggattgagtg atacattgcg
     2401 cgatacggtt gccgcgtggc gcgaaagaca tccggatata acatgcgatt tgcggctgga
     2461 aggcaagctc gacggaatgg gtgaaacgct taatatcacc gtgtatcgca cggttcagga
     2521 atgtctcaca aatatcgcca gacatgctgc agcatcccgt gctgtgatca cagtgactag
     2581 gcaaatgggc aatcgagagg tcgatgatgc ggtgagggtg acggtgagcg acaacggcaa
     2641 aggatttggc gagcagccgt accgtgacgt gatgcgcttt gggcttattg gcatgcggga
     2701 acgcgtccag gcactccatg gtgaattccg gattgagagc acgccgggac agggagcaac
     2761 cgtaacggtc ttgatccctg tggctgcggc ggccggtggc ccccaggggt aatgcggtgg
     2821 aaggcaagtc aatcggaaac attagtgtga tgctggtgga tgaccacgcg gtggtacgca
     2881 tgggttttag attgctgctg gagggttcag ccgatatcaa ggtagtagct gaatcggata
     2941 gcggtgaaga cgcttgtcgg aaatttccgg acgtgaagcc cgacgtggtc gtaatggata
     3001 tatcgatgcc gggtattggt ggacttgaag ctatcgatcg aatattggcg cgcaaccctg
     3061 gcgctcgcat cctggttctt tcggcgcacg aggatgctat gcacgcgcgc agagttttga
     3121 aggcgggggc ggccggttat ctcaccaaac gaagtgcggc tgaagcgttg atccaggcca
     3181 tccggcaggt gcatcaggga aaaacgttcc tggaaccgga gattgcacaa caacttgcca
     3241 tgcaacagct caacggcgag cgcaatccgc tggatatgct aagtgaaaag gagttcaagg
     3301 ttttcctcgc gctggccaag ggccagtccg tggcggatat tgcggacgtg atgtcgctca
     3361 gcccgcgcac cattggcact cacttgtaca acataaagca aaaactgggg gcttcgaatt
     3421 ccgccgagct ggccatcatc gcgattcgcg ctggccttat aaacccatga ctttacagga
     3481 tattgcatgg gatcaatcgc ccagtctcat gaaaatcatt agtcttgttt cagtgtttta
     3541 cgctgggggc tgaagggcag ttagatcaga attgcgccat ggtcgttgat ataagcggcc
     3601 tcgatgcgcg atccgaaatc taacttcaag aggagttgtg ccatgtggac gaaacccgaa
     3661 tacaccgaaa tgcgttttgg ctttgaagta acgatgtacg tgttcaatcg ctaatcgcac
     3721 agcgagacca aaaagggctg ctttcgggca gcccttttcg tttgtggatc cggaaattca
     3781 aaaaccattt gcacatcccg atgccgtttc atctggccga agcctgtcta cccgtctgcg
     3841 attaacatga aataaaccag tcgccctttg cgcaacccat tccccagtcc cagaattgcc
     3901 taccagagag agcacgatga atagaaacgc tttgatgtta gcggccaccg cgatgaccat
     3961 gctgagtgtt tcggttttgg ccgagccgtt tgcctatgtc ccaaatgaag aaagtgggac
     4021 tatttccgtt attgatgtca gtacagatga ggtcgtcaag gaaattcgcg cgggcgacaa
     4081 gcctcgcggc atggcggctt ccaaggacgg caagaaattg tacgtaagcg accagccgca
     4141 caactcgctg gttgtgatcg acctgcaaaa attagagcca gtcgatagta tttttctggg
     4201 cgaatcgcct gaaggagtcg gtatttctcc agatggcaag tgggttgtcg cagcagtaga
     4261 agtcagcaac gccattgctt tcatcaacac ggaaagcaac aagaaggaat t
//
LOCUS       DATMZQ010000102         8122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1810139, whole genome shotgun sequence.
ACCESSION   DATMZQ010000102 DATMZQ010000000
VERSION     DATMZQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810139"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..786)
                     /locus_tag="VN496_03175"
     CDS             complement(130..786)
                     /locus_tag="VN496_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXF15976.1"
                     /translation="MPSRYDLLVFDWDGTLIDSAAHIAVSLQAAFRDLGLRVPSDEAA
                     RHVIGLGLEDTLRYLDPSLEPGRYQEVVDRYRTHFLAGDQIVELFPAVAKGIPVLHQA
                     GYLMAVATGKSRRGLDRSLLATGLNSYFHASRCADEGFPKPNPDMLHEIMRMLGAAPE
                     RTLMIGDTTHDLQMAQNAGVAAVAVAHGAHASANLESMNPLACVEDFEKLMQWLQQNG
                     "
     gene            complement(786..1748)
                     /locus_tag="VN496_03180"
     CDS             complement(786..1748)
                     /locus_tag="VN496_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXF15977.1"
                     /translation="MNSLSKDSASWREVDESAQNQRIDNYLQKILKGVPKSHIYRILR
                     SGEVRVNSGRVDASYRLQDGDKLRLPPIRTAAPKASPLRSSAVFTGSVLLEDEDLLIV
                     DKPSGVAVHGGSGISSGVIEQLRSARPGLRFLELAHRLDRDTSGVLVMAKKRTALTAL
                     HAQLREGAVRKHYVALVLGEWGDAKRNVRAALHKYVAASGERRVVVHDEGQQAHTVFR
                     LIRKFPLLSLLDAELKTGRTHQIRVHLAHIGFPIAGDDKYGDSDANKRLAKQGLKRMF
                     LHAASLEFNHPRNARPVRIEAPLAPELERFVRSLRDAGSAMKEG"
     gene            2108..4693
                     /locus_tag="VN496_03185"
     CDS             2108..4693
                     /locus_tag="VN496_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXF15978.1"
                     /translation="MKRMLFNATQTEELRVAIVDGQKLIDLDIETAGKEQRKSNIYKG
                     IITRIEPSLEAAFVDYGSERHGFLPFKEVSRSFFQNPDADPARVSIREALREGQELIV
                     QVDKDERGNKGAALTTFISLAGRYIVLMPNNPRGGGVSRRVEGEERNELRDTISQLEV
                     PSGMSIIARTAGIGRSVEELNWDLNYLMQLWTAIETAAKQQSGAYLIYQEGSLVIRAI
                     RDYFHQEIGEILIDTEAIYEQARQFMGHVMPANVNRVKLYKDDVPLFSRFQIEHQIET
                     AYSRQVPLPSGGAVVIDHTEAMVAIDVNSARATRGADIEETATRTNLEAADEIARQLR
                     LRDLGGLVVIDFIDMEAARNQRDVENRLRDALRYDRARVQTGKISRFGLLELSRQRLQ
                     TSLGETSHIACPRCHGTGHIRSIESSALHILRILQEEAMKDGTAAVHAQVPIDVATYL
                     LNEKRNEFHSIETRLKVNVLLIPNVGLETPNYTVTRLRHEELNQNDVVLPSYKMADTP
                     VEAVLPLAGAQKPEVQRPQAAVQGITPAQPAPVASSREIAQPSILEKIFGWLTRKPEV
                     VPEPVKPRQRDPRRGRDAEPRRDHRHSRPDTRGEAGKRDEPRRGQRPDADNRDQGRNR
                     GQQHAERKPQDRPQRPQRPDQQLAAVEQRQPGDRPAESAREEGRGRRRGRGRRERHVE
                     DNGHAQPRGEQHNRASSGQAANAPGPARPPREQRDHGAADMARDESRATMNEQQPVQP
                     QEQVVAAQSVTVQESFAREAITESAMTAEYTPVVVIPEQRPLPEPAVNAQSASGWKMD
                     PVTLPSELIMIETQHKASSANEEPEMPRRTRTPRPRMQSAQVSDEPLQQVETGKRQSA
                     GNDAP"
     gene            complement(4737..6830)
                     /gene="uvrB"
                     /locus_tag="VN496_03190"
     CDS             complement(4737..6830)
                     /gene="uvrB"
                     /locus_tag="VN496_03190"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXF15979.1"
                     /translation="MTLTFPNSPFRLHQPFKPAGDQPEAIASLVEGLNDGLAFQTLLG
                     VTGSGKTYTMANVIAITGRPAIVMAPNKTLAAQLYAEMREFFPENAIEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLLERDDAVIVATVSAIYGIGDPVDYH
                     GMILHVREKERMSQRDIISRLTEMQYDRNDFEFRRGAFRVRGDIVDVFPAENSEMALR
                     IALFDDEIEALTLFDPLTGHLVQKVPRYTVYPSSHYVTPRSTTLRAIEAIKEELRLRI
                     ETFQSSGKLVEAQRIEQRTRFDLEMMNELGFCKGIENYSRHLSGRKPGEPPPTLLDYL
                     PLNALMFVDESHVTIPQVGGMYRGDRARKENLVEYGFRLPSALDNRPLRFDEFERIMP
                     QAIFVSATPAEYEKEHQGQVVEQVVRPTGLIDPEIEVRPAATQVDDLLSQITERVASG
                     QRVLVTTLTKRMAEDLTDYLAEHGVKVRYLHSDIETVERVEIIRDLRLGEFDVLVGIN
                     LLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHLNGKAIMYADVMTDSMR
                     RAIAETDRRRLKQISYNTAHGITPIGVSKRVRDMIEGVYDSSVQQDRKAAEKLAAYGA
                     MNEKDLLRELKRVEREMLESARNLEFERAAQLRDQLKSLKELAFGVAEIGDELPATGT
                     HGQVMAIASARKKKA"
     gene            6868..8085
                     /locus_tag="VN496_03195"
     CDS             6868..8085
                     /locus_tag="VN496_03195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HXF15980.1"
                     /translation="MNAPHRASPLAAVQMAPKDPILGVTETFNADKNPHKVNLGVGIY
                     YDDSGKVPLLECVRQAEIRLAEKSAPRSYLPIDGLPAYDRAVMELVFGSNCPAVKENR
                     VITVQALGGTGALKIGADFLRRVLPQAEVWISDPSWENHRALFENAGFRVNTYPYYDA
                     ASHGIDFSGMLNALKSLAADSIVVLHACCHNPTGVDLSVSQWAKVIDVVVSRGLMPFV
                     DIAYQGFGDGIHADGEVVRRFAEAGCPVFVSNSFSKSFSLYGERVGALSVVAGDATEA
                     ARVLSQLKRIVRTNYSNPPTHGGQIVATVLTSPELRALWEKELGEMRGRIQEMRRLLV
                     EKLRTRLPDSDFGFVTRQRGMFSYSGLSKAAVDRLRSEFSVYAIDTGRICVAALNSRN
                     IDHVTGAIAKVAG"
BASE COUNT         1773 a         2381 c         2272 g         1696 t
ORIGIN      
        1 cgcagctatt caggtaggct tggaccttgc ctcgatggcg cacgacaaag gcaggtttta
       61 ccttgccgat ggcgccgacg ctgaaacgca ctcccgtgcc gcgttccggc aagtcctgtg
      121 agagacaaat caaccgtttt gctggagcca ttgcatcaac ttttcgaaat cctctacgca
      181 ggcgagggga ttcatgctct ccaggtttgc tgaagcatgg gctccatggg caaccgccac
      241 ggccgccacg ccagcgtttt gcgccatctg caagtcgtga gtggtatcgc cgatcataag
      301 agtacgttcc ggcgcagcgc ctaacatccg catgatctcg tgaagcatgt ccgggtttgg
      361 cttgggaaac ccttcgtctg cgcatcgtga ggcatggaaa tacgaattca atccggtagc
      421 cagcaacgac cgatcaaggc cacgcctgct tttacccgtg gcaacagcca tcagataccc
      481 tgcctggtgt agcaccggaa tgccttttgc cactgcagga aacagctcga cgatttgatc
      541 gccagcaagg aaatgtgtgc gatatcgatc gaccacttcc tggtaacggc caggctccaa
      601 cgacgggtcc agataccgca aggtatcttc gagtcccagc ccaatgacat gtcgtgccgc
      661 ttcgtcagat gggacccgaa ggccaagatc ccgaaaggcg gcctgcagtg aaacggcgat
      721 gtgtgcggcg gaatcaatca gcgtgccatc ccaatcgaat accagcagat catagcggct
      781 cggcattacc cttccttcat cgctgagcca gcatctcgca gcgatctgac gaaacgctcc
      841 agttccggtg cgagaggcgc ttcaatgcgc acgggtcgcg catttcgggg atggttgaac
      901 tcaagacttg cagcgtgcag gaacatgcgt ttgagtcctt gcttcgctag ccgtttgttg
      961 gcatcggaat ctccgtattt gtcatcgccc gcaatcggaa atccgatatg agccagatga
     1021 acccgaatct ggtgcgtgcg accggttttt aattcggcgt ccagcaagct caacaacgga
     1081 aactttctga tcagccggaa tactgtatgg gcctgctgtc cttcatcgtg cacgacgacc
     1141 ctgcgttccc cggaagccgc gacatatttg tgcaaagcgg ctcgcacgtt gcgcttcgcg
     1201 tcaccccatt ctcccagtac cagcgcgacg tagtgtttgc gcacagcacc ctcgcgcagc
     1261 tgcgcgtgca gtgcagtaag ggcggtacgc tttttggcca ttaccagtac gccagatgta
     1321 tcccggtcca accggtgcgc cagttcgaga aaacgcaagc ccggccgggc gctgcgcaat
     1381 tgctcgatca cgccacttga aatgccactg ccgccatgta cggccacgcc gctcggtttg
     1441 tcgacaatca gcaagtcttc gtcttccagc agcacgctgc cggtaaaaac cgcggatgac
     1501 ctcaatggag aagctttagg cgctgctgtc cggatgggcg gcagccggag tttatcgccg
     1561 tcctgcaacc ggtaggacgc gtcaacccgc ccactgttca cccgaacttc accgctgcgc
     1621 aggatgcggt agatgtggct tttgggcaca cctttcagga tcttctgcag ataattgtcg
     1681 atgcgctgat tctgtgcgga ttcatctact tcgcgccatg atgcagagtc tttgcttaag
     1741 ctgttcattt tccttatact aatcaaacga acgggcaatc agtgcaaatt ctgtggtcga
     1801 gccctgccgc cggcgcacgc cgtgcttgga gctcccgcag atctgcggcc gtcgaagaga
     1861 ggggttaagc tcgctcaccc gaaaaagtag cgatggtaaa tcatgagttg cgcgctcgca
     1921 gcattccagt ggattttcac agtcctgcta atgcaggatg aacaacagac aaaccttcag
     1981 gcgacaatag ctatcgtcct ccacaaccca tgagtcatcc gacgacggaa agccaataag
     2041 ccgcgcgcct taacggcgct gcaaggtttg tcctccccgt gaatagtgag cgcgggagag
     2101 caaaataatg aagaggatgt tgtttaacgc gacccagaca gaagaattgc gtgtcgcgat
     2161 cgttgatggc cagaagctca tcgacctcga tatcgaaaca gccggaaaag aacaacgcaa
     2221 gagcaacata tacaaaggca tcatcacccg catcgaaccc agcctcgagg ccgcctttgt
     2281 ggattacggc agcgagcgcc atgggtttct gccgtttaag gaagtcagcc gctccttctt
     2341 tcaaaatccg gatgccgatc cggcccgggt ctccatcagg gaagcgttgc gcgagggcca
     2401 ggaacttatc gtccaggtcg acaaggacga gcgcggtaac aagggagcgg cgctcaccac
     2461 cttcataagc ctcgcaggtc gttacatcgt actgatgccc aataatccac gcggcggcgg
     2521 tgtatcgcgt cgggttgaag gcgaagagcg caacgaattg cgcgatacga tttcccagct
     2581 cgaagtgcct tccggaatga gcatcatcgc tcgtacagcg ggcatcggcc gctcggtaga
     2641 ggaactgaac tgggacctga attacctgat gcagttgtgg actgccatcg agaccgccgc
     2701 gaagcagcaa agcggcgctt atctgatcta tcaggaagga agcctggtca tccgggcaat
     2761 ccgcgattat ttccaccagg aaatcggtga aatcctgatc gataccgagg cgatctacga
     2821 gcaggcaagg cagttcatgg gccatgtcat gccagcgaat gtcaaccgcg tcaagctgta
     2881 caaggatgat gttccgctat tttcgcgctt ccagattgaa caccagatcg aaaccgccta
     2941 ttcccgccag gtgccccttc cctctggcgg cgcggttgtg atcgatcata cggaagcaat
     3001 ggtagccatc gatgtcaact cggcccgcgc cactcgcggt gcggacatcg aagagacagc
     3061 gacgcgcacc aacctcgagg ccgccgatga gatcgcgcgc cagcttcggt tacgcgacct
     3121 tggcggcctg gtcgtaatcg acttcatcga catggaagcg gcccgcaacc agcgggacgt
     3181 tgagaaccgg ctgcgtgatg ccttgcgcta tgaccgtgcc cgcgtccaaa ccggaaagat
     3241 ctcgcgcttt ggcttgctgg aattatcgcg ccagcgtttg caaacctcgc tgggagagac
     3301 cagtcacatt gcatgtccgc ggtgccacgg aactggacat atccgctcga ttgaatcttc
     3361 ggctttgcac atcctgcgga ttcttcaaga ggaagccatg aaggacggca ccgcagccgt
     3421 gcacgcccag gtccccattg acgttgccac ctatctgctc aatgagaagc gcaatgagtt
     3481 tcactccatt gaaaccaggc tgaaagtcaa cgtactgctg attccgaacg tcggtttgga
     3541 gactccgaac tacaccgtga cccgtttgcg gcatgaggaa ctcaatcaga acgatgtcgt
     3601 cttgccgagc tataagatgg ccgatacacc ggtcgaagcg gtgctgccct tggccggcgc
     3661 gcagaaaccg gaagtccagc ggccgcaagc tgcggtgcaa gggattaccc cggcgcagcc
     3721 cgcgcctgtg gcttcgtccc gagagatcgc gcaaccgtcc atccttgaaa aaatattcgg
     3781 ctggttgacc cgcaagcctg aagtcgttcc ggagccggtg aagccgcggc aacgcgatcc
     3841 acgccgcggc cgggacgctg aaccgcgccg cgaccaccgg cacagtcgtc cagatacccg
     3901 aggcgaggcc gggaaacgtg acgaaccgcg acgaggtcaa cgtcccgatg cagacaaccg
     3961 cgatcagggg cgaaatcgcg gtcagcaaca cgccgagcga aagccacaag atcgcccgca
     4021 gcggccgcaa cgtcctgacc agcaattagc cgccgtcgag caacggcagc caggtgatcg
     4081 tcccgcagaa tcagctcggg aggaaggacg aggtagaaga cgcggtcgcg gtcgccgcga
     4141 gcgccatgtt gaagataacg ggcatgcgca gccacgcggc gagcagcaca accgggcgtc
     4201 ttccggtcag gctgctaatg cgccgggacc agccaggcct ccacgtgagc aacgggacca
     4261 cggcgcagcc gacatggctc gcgatgaatc cagagcaaca atgaacgaac aacagccggt
     4321 acagccgcag gaacaggtgg tcgccgcgca atccgttacg gttcaggagt cgtttgcgcg
     4381 cgaagcaatc accgaaagtg caatgactgc tgaatatacg ccagtggtcg ttattccgga
     4441 gcaacggccg ttaccggaac cggcagtcaa tgcgcaatca gcctctggct ggaaaatgga
     4501 tcctgttacg ctgccttcgg aactcatcat gatcgaaacg cagcacaaag cgtcttcggc
     4561 caatgaggaa ccggaaatgc cccgtcgcac gaggacaccc cgcccgcgaa tgcaatcggc
     4621 gcaagtttcc gacgagccgc tgcagcaagt ggaaactggc aagcgtcaat ccgctggcaa
     4681 cgacgcgccc tgattcaaga acaagaacgt tgcactaaga gcccaatccg ggctctttag
     4741 gctttcttct tgcgcgccga tgcaatggcc atgacctggc cgtgcgtacc ggtagcaggc
     4801 agttcatcgc cgatttcggc gactccgaaa gccaattcct tcaagctctt cagttggtct
     4861 cgtaactgtg ccgcgcgctc gaactccaga tttctggcac tttccagcat ctcgcgctcg
     4921 acgcgtttca gttcgcggag caagtctttc tcattcatcg caccgtaggc cgccagcttt
     4981 tctgccgcct tacgatcctg ttgcaccgaa gaatcgtata cgccttcaat catatcccgc
     5041 acgcgctttg agacaccgat tggcgtaatg ccgtgcgctg tgttgtagct gatctgcttc
     5101 aaacgcctgc ggtccgtttc agcgattgcg cgccgcattg aatcggtcat cacatcggca
     5161 tacatgatcg ctttgccgtt caagtgccgg gcggcccggc ctatggtctg gatcagcgat
     5221 cgctccgagc gcaggaagcc ctccttgtcg gcatccagaa tcgccaccag ggagacctcg
     5281 ggaatatcga ggccttcgcg cagaagattg attccgacca gcacgtcgaa ctctccgaga
     5341 cgcaagtcgc ggatgatctc cactcgctcc accgtttcga tgtcggaatg cagatagcgc
     5401 accttgacac cgtgctccgc caggtagtca gtcaaatctt cggccatgcg tttggtcagt
     5461 gtcgttacca agacccgctg accgcttgca acccgttcag tgatctgcga cagcaaatcg
     5521 tccacttggg ttgcggccgg gcggacctcg atttccggat cgatcagccc ggtaggccta
     5581 acgacctgct ctacgacctg gccctgatgt tctttctcgt attctgccgg cgtcgcggac
     5641 acgaatatgg cctgcggcat gattctttcg aattcatcga agcggagtgg ccggttatcg
     5701 agtgcactgg gcaaccggaa tccgtattcc accagatttt ccttgcgggc acgatcacct
     5761 ctgtacattc ctccaacctg cggaatggtt acgtggcttt cgtcaacgaa catcagagcg
     5821 ttcagaggca ggtaatccag caatgttggc ggcggctcac caggtttgcg tccggataaa
     5881 tggcgggaat aattttcgat ccccttgcaa aagccaagtt cgttcatcat ctcgaggtca
     5941 aagcgcgtgc gttgctctat gcgctgtgcc tccaccagtt taccgctgct ctggaatgtc
     6001 tcgatgcgca gccgcagctc ctccttgatc gcttctatgg cacgcagtgt ggtacttcgc
     6061 ggagtcacgt agtggctgga tggataaact gtgtagcgcg ggactttttg aacaagatgg
     6121 ccggtcagag gatcgaacag cgtcagggct tcaatttcgt cgtcgaataa cgcgatccgc
     6181 aatgccattt ccgagttctc ggccgggaat acgtcaacga tgtcgccacg cacacgaaac
     6241 gcgccacggc ggaattcgaa atcattgcga tcgtattgca tttcggtcag ccgtgaaatg
     6301 atgtcacgct ggctcatgcg ctccttttcg cgcacatgca ggatcatgcc atggtaatcg
     6361 accggatcac ctatcccgta gatggccgag actgtcgcga cgatgactgc gtcgtcccgc
     6421 tccagcagcg atttggttgc tgacaaccgc atctgctcga tatgttcatt gatgctcgaa
     6481 tccttttcga tgaacaaatc gcgcgatggc acgtaagctt caggctgata gtagtcgtaa
     6541 taggaaacga aatactcaat ggcattctcg ggaaaaaact ctctcatctc agcatacagc
     6601 tgcgccgcaa gagttttgtt tggcgccatc acgatcgccg gccggccagt gattgcgata
     6661 acgttggcca tcgtatacgt cttgcccgaa ccggttacgc cgagcagcgt ctggaacgca
     6721 agaccgtcgt tcagaccctc caccagagag gcaatcgctt ctggctgatc cccagcgggc
     6781 ttgaacggct ggtgaaggcg aaacgggctg ttgggaaaag tgagtgtcac ggtacaatac
     6841 ggccttaaaa attttcgata ttacacgatg aacgctcctc accgcgcgtc acctctggca
     6901 gccgtgcaaa tggctccgaa ggatcccatt ttgggggtga ccgaaacctt caacgccgac
     6961 aaaaatccgc acaaggtcaa tctcggggtc ggcatctatt acgacgacag cggcaaggtg
     7021 cctttgcttg agtgcgtccg tcaggcggaa atccggcttg ccgaaaaatc ggctcctcgc
     7081 agctatctgc ccatcgacgg tctgccggct tatgaccgcg ccgtcatgga attggttttc
     7141 ggcagcaact gtccagccgt gaaggaaaat cgtgtgatca cagtgcaggc gctcggaggc
     7201 acgggtgcgc taaaaatcgg cgcggatttt ctgcggcgtg ttctgcctca agcggaggtc
     7261 tggatttcag atccaagctg ggaaaatcat cgcgcgctgt ttgagaacgc gggctttcgg
     7321 gtaaatacgt atccctacta cgatgcagcc tcgcacggca ttgatttcag cggcatgcta
     7381 aacgcgctaa agtcgctggc cgccgacagc atcgtcgtat tgcatgcgtg ttgccataat
     7441 cccaccggcg tggatctgtc ggtctcgcaa tgggccaagg tgatcgatgt cgtggtttcc
     7501 cgaggtctca tgccgttcgt cgatattgcc taccagggat ttggcgacgg tatccacgcc
     7561 gacggtgagg tggttcgccg ttttgctgaa gccggctgcc ccgtgttcgt ctcaaattca
     7621 ttttccaaat ccttctccct gtacggtgag cgtgtcgggg cactcagcgt cgttgccggc
     7681 gacgcaactg aagcggcccg cgtactgagc cagctgaaac gtatcgtacg caccaactat
     7741 tccaacccgc caacgcacgg cggccagatc gtcgcgactg tgcttaccag cccggagctt
     7801 cgcgctttgt gggaaaagga actgggtgag atgcgcggtc gcatccagga gatgcgtcgc
     7861 ctgctggtcg agaagctgcg tacgcgtctg ccggactcag acttcggctt tgtgacccgg
     7921 caacgtggca tgttttctta ttccggactg tcaaaagcgg cagttgatcg tttgcggagt
     7981 gagttttcag tttatgcgat agacaccgga cgcatttgcg ttgcggcgct aaattcccgc
     8041 aacatagacc atgttacagg cgctatcgcc aaggtagccg gttgaccgtt cagcgttaca
     8101 tatgtgatgg tgcagcgagc gt
//
LOCUS       DATMZQ010000103         3529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1811898, whole genome shotgun sequence.
ACCESSION   DATMZQ010000103 DATMZQ010000000
VERSION     DATMZQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811898"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VN496_03200"
     CDS             complement(<1..514)
                     /locus_tag="VN496_03200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF15981.1"
                     /translation="MDPNKFTNFGFQTVREDEKARKVAGVFSAVANRYDIMNDLMSAG
                     LHRLWKRFAVELSAVRKGGRVLDVAGGTGDLSLLFASRVGETGQVWLTDINREMLMRG
                     RDRLIDAGRLTPVLQSDAEHLPLPDSYFDCAIVGFGLRNMTHKEKALAEMLRVVRSGG
                     RVLVLEFSQPW"
     gene            complement(528..953)
                     /locus_tag="VN496_03205"
     CDS             complement(528..953)
                     /locus_tag="VN496_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXF15982.1"
                     /translation="MAGLQPGTPRPIEIKLHQKSRLLEIGFDDGKTFQLSCEYLRVYS
                     PSAEVRGHGPGQEVLQEGKKDVEITAIEPVGNYAVKLVFNDGHDTGLYSWDYLHHLGE
                     NQPSLWQRYLERLAVAGLHREPVGKADAGPRDKSGWKKI"
     gene            1096..1782
                     /gene="phoB"
                     /locus_tag="VN496_03210"
     CDS             1096..1782
                     /gene="phoB"
                     /locus_tag="VN496_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HXF15983.1"
                     /translation="MAATVLVVEDEPAIQELIAYNLKQAGHQPLRADNAEQALVLVAS
                     ALPDLVLLDWMLPGLSGVELAKRLRADKRTRAIPIIMLTARSEEQDKLQGLEIGADDY
                     ITKPFSPRELNARIKAVLRRRAPEMTDDLVKLGGLSLDPASHRVTGKGQPIDLGPTEF
                     RLLHFLMTHPERVHSRTQLLDQIWGDHVFVEERTVDVHIRRLRKALEPTHLDGLVQTV
                     RGTGYRFSAT"
     gene            1849..3183
                     /gene="phoR"
                     /locus_tag="VN496_03215"
     CDS             1849..3183
                     /gene="phoR"
                     /locus_tag="VN496_03215"
                     /inference="COORDINATES: protein motif:HMM:NF008235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HXF15984.1"
                     /translation="MNFLWGRPLSTVFLIGVIVLLSWPLLGHGGALLFLCMMLLGLLM
                     HHLRNLSLLYRWLQHPQSHSLPTGSGAWEYLSSHMLKMLKRQRETEARLNEALSRFQL
                     AGAALPDAVVILDGADRIEWCNPSAENYFGLDNNRDRGQQITYILRQPQFVQHLASEH
                     AGDPLVLRMSRPAGEMVLAVQQVPYGENHRLLLGRDITRWERLETTRRDFVANVSHEL
                     RTPLTVVGGFLETLADMEVPDPEMSRRAIGLMRQQTSRMTRLVEDLLTLSRLESAQSP
                     SREEDVNVPEMVRALLHEAQALSAGHHQIRLVVESADWLKGNTEELRSAFSNLVSNAV
                     RYTPDNGAIEISWKVRDTHLVFAVKDNGIGIEPQHIDRLTERFYRVDRSRSRETGGTG
                     LGLAIVKHVVNRHQGLLDIQSTPGSGSTFSVLFPETRRLATGRPATPAKMLA"
     gene            complement(3180..3464)
                     /locus_tag="VN496_03220"
     CDS             complement(3180..3464)
                     /locus_tag="VN496_03220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXF15985.1"
                     /translation="MTDLSDADVTRLMRLVFAVERSLRILLSPEKINLASLGNMVPHL
                     HWHVIPRFAGDPHFPNAVWSAKARDGARALPADFTAMLRRQIDETLLLKH"
BASE COUNT          731 a         1051 c          998 g          749 t
ORIGIN      
        1 gccagggttg cgaaaattcc agcaccagca cacgtccccc gctgcgcaca acacgaagca
       61 tctcggcgag tgccttctcc ttgtgcgtca tgttgcgcaa accgaatccc acgatggcgc
      121 aatcgaaata actgtctggc aaaggcaggt gctcggcgtc gctctgcaga accggtgtga
      181 gccggccggc atcgatgagg cgatcgcgtc cgcgcatgag catttcgcgg ttgatatcgg
      241 tcagccagac ttggcccgtt tcgccaacac gggaagcaaa caacaatgac agatcaccag
      301 ttccgccagc gacatccagc acccggccgc ctttgcgcac ggcgctcagc tcgaccgcaa
      361 agcgtttcca tagccggtgc aggccagctg acatcaagtc attcatgatg tcgtagcggt
      421 tggccactgc gctgaagaca ccagctactt ttctggcctt ttcgtcttcc cgcaccgttt
      481 ggaagccaaa atttgtgaat ttgttgggat ccatagtgct ttcgcgttca gattttcttc
      541 caacccgatt tgtcgcgcgg cccggcgtca gctttgccga ccggctcccg gtgcagaccg
      601 gcaaccgcga gtctttcaag atagcgctgc cacaagctcg gctgattttc cccgagatga
      661 tgaagatagt cccaggaata cagccccgtg tcgtgcccgt cgttaaatac cagcttgacg
      721 gcgtagttgc cgaccggttc tattgcagtt atttctacgt cttttttgcc ttcctgcagg
      781 acttcctggc cggggccatg tccgcgcact tctgccgacg gagaataaac gcgcagatac
      841 tcgcacgaca gctgaaatgt tttgccgtcg tcaaagccga tttccagcag ccgcgacttc
      901 tggtgcaact ttatttcgat gggacgaggg gtccccggtt gaagtcctgc cacggctctg
      961 gaaaagttcg attgttgcag ttgaaattga catccatcat agcggcaagc aagcgtacat
     1021 cgcctgcccg tcatgcgctt gtaacatttg cttcatagta ttgcaaacgt aaaaaaacgg
     1081 gggaagacat cgtttatggc agcaaccgtg ctggtcgtcg aagatgaacc agcgattcag
     1141 gaattaatcg cctataacct gaaacaggcc ggtcaccagc cattgcgcgc agacaatgca
     1201 gagcaggcac tggtgctggt cgcgagtgcg ttgcccgatc ttgttctact cgactggatg
     1261 ctccccgggc tttcaggcgt ggagctcgcc aaacggttgc gcgccgacaa gcgcacccgc
     1321 gcgataccga tcatcatgct gaccgcgcgt tccgaggaac aggacaagct gcagggattg
     1381 gagatcggcg ccgacgatta catcaccaaa ccattttctc cgcgcgaatt gaatgcgcgt
     1441 atcaaagcgg tattgcggcg gcgcgcgccc gagatgacgg acgacctggt caaactcggc
     1501 ggattgagcc tggacccggc cagtcaccgc gtgaccggaa aaggccagcc gatcgattta
     1561 ggccccaccg aattccgcct gctgcatttt ctgatgacgc atcccgagcg tgtccattcg
     1621 cgcacacaat tgctggacca gatttggggc gaccatgtct tcgtcgagga acgcaccgtg
     1681 gacgtgcaca ttcgtcggct gcgtaaggca ctcgaaccca cccatctgga cggactcgtg
     1741 cagactgttc gcggcaccgg ctatcgcttt tccgcgacct gatggtgccg tctggacgag
     1801 gcgtaattct gcgataatgc cccgcgtctc attcgaggga caaaccgctt gaactttctg
     1861 tggggcaggc ccctcagcac ggtcttcctg atcggagtaa tcgtgctact gtcctggcca
     1921 ttgctggggc atggcggcgc gctgctgttc ctctgcatga tgctcctggg tttgctgatg
     1981 catcatttgc gcaacctctc cttgctctat cgctggttgc agcatccgca atcgcactcc
     2041 ctgcccacgg gttccggtgc ctgggaatat ttgtcgtccc atatgctgaa aatgctcaag
     2101 cgccagcgcg aaacggaggc caggctcaac gaggcgctgt cgcgattcca gcttgccggc
     2161 gccgcgcttc ccgacgcagt ggtcattctc gatggcgccg accgcatcga atggtgcaat
     2221 ccaagtgcgg agaattattt tggcctcgac aataaccgtg accgcggcca acagatcaca
     2281 tatatcctcc gccaaccaca gttcgtgcag cacctggcct ccgagcatgc tggtgatcct
     2341 ctcgttctga gaatgtcgcg accggctggc gaaatggtcc ttgccgtgca gcaggtgcct
     2401 tatggcgaga accaccgatt gttgctgggc cgcgatatca cgcgctggga gcggctggag
     2461 acgacacggc gcgatttcgt ggccaacgtt tcgcatgaat tgcgcacgcc gcttaccgtc
     2521 gttggcggct ttctggaaac actggccgac atggaagtgc cggatccgga aatgtccagg
     2581 cgcgcgatcg gattgatgcg gcagcagacc agccgcatga ccaggctggt ggaggatttg
     2641 cttactctgt cacgtctgga gagtgctcag agtccgtcgc gcgaggaaga cgtcaatgtc
     2701 ccggagatgg tacgtgcgct tctgcacgaa gcacaggcac tgtccgccgg ccaccatcaa
     2761 atccgcctgg tcgtcgagag cgccgattgg ctgaagggca atactgaaga attgcgtagt
     2821 gccttcagca atcttgtcag caatgcggtc cgctataccc ctgacaacgg tgccatcgag
     2881 atcagttgga aagtgcgcga tacgcatctg gttttcgcag tcaaggacaa tgggattggc
     2941 atcgagccgc agcacatcga ccgcttgact gaacgtttct atcgcgtgga ccgcagccgt
     3001 tcgcgcgaaa caggaggcac agggctcggg ctcgccatcg tgaagcacgt ggtaaaccgt
     3061 caccagggcc tgctggacat tcaaagtaca ccaggcagcg gcagcacgtt ttccgtccta
     3121 tttcccgaga ctcgccgcct ggcgactggg cggcccgcga cgcccgcgaa aatgttggcc
     3181 tagtgtttca acagcagcgt ctcatcaatc tgccggcgca gcatcgcagt gaaatccgcg
     3241 ggcagcgcac gcgcaccatc gcgcgctttt gccgaccaaa cggcattcgg aaaatgcggg
     3301 tcgcccgcaa atcttggtat cacgtgccag tgcagatggg gcaccatgtt gccaagactg
     3361 gccagattga ttttctcggg cgacagtaag atcctcagac tgcgttctac ggcgaataca
     3421 agacgcatta gccgtgtgac atcggcgtcg gacaggtcag tcatttactt ctcatgcgca
     3481 ttcaagatca cccggcagaa accagggtgg tctgcatcgc caaccagta
//
LOCUS       DATMZQ010000104         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4186097, whole genome shotgun sequence.
ACCESSION   DATMZQ010000104 DATMZQ010000000
VERSION     DATMZQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4186097"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VN496_03225"
     CDS             <1..415
                     /locus_tag="VN496_03225"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HXF15986.1"
                     /translation="VGCIFERQRNRTVNESEVARKIAARLDQDLADLPEPMVRKLRDA
                     RTAALAAYRPERPDRKAGMVAGWIFDHGMTARLMLPAAVVVAAITGLIYWQMSSHHEG
                     ELDTGLLAGELPIHAYTDPGFETWLKHSNYTQSRP"
     gene            complement(610..810)
                     /locus_tag="VN496_03230"
     CDS             complement(610..810)
                     /locus_tag="VN496_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15987.1"
                     /translation="MAFAKLLPLFFRQCLVFTPTLVQLLVLLWWKLFHAFVALDSLRA
                     LFRRQGDPFMHTLLNPLPALGR"
     gene            871..1350
                     /locus_tag="VN496_03235"
     CDS             871..1350
                     /locus_tag="VN496_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXF15988.1"
                     /translation="MRPHGLPLKPLPLAPFRLRLMSLVYEAMLLFGILFVCSYVFLSV
                     AREALSGWHRVLFQFYLLCVCGAYFVFCWARTGQTLALKTWRMRVTREDGQPLTITRA
                     LWRYLLCIPGMLSGTSLLWAVFDKERQFLHDRLAHTRIVRTETSQLSAAERSNNPPG"
     gene            complement(1373..2449)
                     /gene="lptG"
                     /locus_tag="VN496_03240"
     CDS             complement(1373..2449)
                     /gene="lptG"
                     /locus_tag="VN496_03240"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HXF15989.1"
                     /translation="MKTIRRYVAVQIFWSTALVFAALLLLFGFFDFIQELGDIGRGSY
                     RLSLAALYVILSLPGRAYEILPVAALIGTLFALAQLVAHSEYTVMRVSGVSIKNMAMA
                     LVQIGIGLSVLTFLIGEFLAPASEQAAQNLRLKATSSTVVAQAFRSGLWVKDDTNFVN
                     VGRIMPETVIHEVKIYEFDADYRLRAISHAKLGEYQQENLWRLKEVVQTRFEENRTTV
                     STIPEAYWRSVLNPSILNVLMVVPEQMSLMELISYIEHLSENHQESSRYEIVLWQKII
                     YPFAVLVMMVLALPFAYFQVRERGLSGRVFTGIMLGLGFHLANRLFGHLGLLNAWPPL
                     FSAVFPTVAFFGAAMVMMWKVEKR"
     gene            complement(2446..>2886)
                     /locus_tag="VN496_03245"
     CDS             complement(2446..>2886)
                     /locus_tag="VN496_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXF15990.1"
                     /translation="GPPGSAEFRIVEFEKYSVRIEANEVKDTLPSVKALPMMSLLADR
                     SAEAHAELVWRTGLPLSALVLSLLAIPMSFVNPRAGRSLNLMLAALLYMVYTNLVSIV
                     QAWVAQEKVGVLLGMWSLHAAMLMIVAVMFYLRVSLRPLVLRKR"
BASE COUNT          691 a          823 c          776 g          596 t
ORIGIN      
        1 cgttggctgc atttttgaaa gacaaaggaa tcggactgtg aacgagagcg aagttgccag
       61 aaagattgct gcgcgcctcg accaagacct ggcggacttg ccagaaccca tggttcggaa
      121 acttcgggac gccagaacgg cagccctggc agcgtatcgt ccagaaaggc cagatcgcaa
      181 agcgggaatg gtcgctggct ggattttcga ccatggcatg accgcgcgct tgatgttgcc
      241 cgcggcagtt gtagtcgccg cgattaccgg gttgatctat tggcaaatgt cgagtcacca
      301 tgaaggagaa ctggacaccg gcctgctggc tggcgaactg cctatacacg cctataccga
      361 tccgggattt gaaacttggt taaagcactc caattacacg cagtcgcgac cgtagtcctg
      421 actctactgc tgtcggtatt cgttggaaca atgtcggggc tccccgcgta tgcggaatcc
      481 ccgggcgcgg cgtggtcgaa actgaccccc gaacagcaat ctattctctc tccgcttcag
      541 acggaatggg acaaattgga ttctgagcgt cgtaaacact ggattggtct ggccggtcgc
      601 tatccgaatt tatcgcccga gcgccggcag cggattcagc agcgtatgca tgaatgggtc
      661 gccttgacgc cggaacaacg cgcgcaggct atcgagcgct acaaacgcat gaaacaactt
      721 ccaccagaga agcacgagca actgcaccag cgttggcgtg aataccaggc actgccggaa
      781 gaacagcggc agcaacttcg cgaaggccac ggtacagcca ctagcccagc caagtgatca
      841 ctctataacg gcgatcgcta atttagatcc atgaggccac acggcctgcc actaaaaccc
      901 ctgccactgg cgcccttccg tttgcgcctg atgagtttgg tgtacgaagc aatgttgctg
      961 tttggcatcc tgtttgtttg ttcctacgtc tttttgagtg tcgcgcgcga agccctatcc
     1021 ggctggcacc gcgtgttgtt ccagttttat ttgctctgcg tatgcggcgc gtatttcgta
     1081 ttctgctggg cgcgcacggg tcaaacattg gcgctgaaaa cctggcgcat gcgagtcacc
     1141 cgggaagacg ggcaaccgct gacaatcacc agagcattgt ggcgctatct gctttgtata
     1201 ccggggatgc tgtccggcac cagcctgctt tgggctgtgt tcgacaagga gcgccagttt
     1261 ctgcatgacc ggctcgctca caccagaatc gtccgcaccg aaacttcaca actgagcgcg
     1321 gcagagcgtt cgaataatcc gcctggttag cgcgacaaag tggtttacac cttcatcttt
     1381 tttccacttt ccacatcatc accatcgcgg ctccgaaaaa tgcgacggtg ggaaaaaccg
     1441 cactgaacaa tggcggccac gcattgagca agccaagatg gccgaacaag cggttggcaa
     1501 gatgaaatcc aagcccaagc atgatgccgg tgaatactcg gccgctcagg ccgcgctctc
     1561 gcacctgaaa ataggcgaac ggtaaagcaa gcaccatcat gaccagcaca gcgaaagggt
     1621 agatgatttt ttgccaaagg acgatttcat aacgggatga ctcctggtga ttttcactga
     1681 ggtgctcgat atacgatatg agctccatca gcgacatctg ctccggcact accatcagga
     1741 cattcagaat ggagggattg agcacggagc gccagtaggc ctccggaatc gtgctcacgg
     1801 tcgttcggtt ctcctcgaac cgggtttgaa ccacttcttt gagcctccag agattttctt
     1861 gctgatattc gcccaatttc gcatgactga ttgcacgcag ccggtaatcc gcgtcgaact
     1921 catagatttt cacctcatgg attactgtct ccggcatgat gcgcccgacg tttacaaagt
     1981 tcgtgtcatc cttgacccag aggcccgaac gaaacgcttg agcaacgacc gtgctggaag
     2041 tggctttgag ccgcaagttt tgagccgcct gctcgcttgc cggcgccaaa aattctccaa
     2101 tcagaaaggt cagtacggat aaccctattc caatctgcac cagcgccatc gccatgttct
     2161 ttatcgaaac gccggacacc cgcatcacgg tgtattcgga atgcgccacc aattgcgcaa
     2221 gggcgaataa cgtcccgatc aatgccgcga ccggcaaaat ttcgtatgcc ctgccgggaa
     2281 ggctcagaat cacgtacagc gcagcaaggg acaggcggta actgccgcgt ccgatgtcac
     2341 ccagctcctg gataaagtcg aaaaatccaa acagcagcag caaggctgca aacactaacg
     2401 cagtgctcca aaagatttgt actgcgacgt agcggcgaat cgtcttcatc gctttcgcag
     2461 gacaagcggg cgcaaagaga cccgcaaata aaacatcacc gcgacgatca tgagcatggc
     2521 cgcatgcagg ctccacatgc caagcagcac gccaactttt tcctgggcga cccacgcttg
     2581 aactatggat accagattgg tatagaccat ataaagcagc gccgcgagca tgagattgag
     2641 cgagcggccg gcacgcggat tgacaaaact catcggaatc gccagcaagc tcaagaccag
     2701 cgcgctcaat ggcaaaccgg tgcgccagac aagctccgcg tgtgcctctg cggacctgtc
     2761 ggccagtagg gacatcatgg gcagcgcctt gacggagggc agcgtgtcct tgacttcgtt
     2821 ggcttcgata cggactgaat atttttcaaa ctccacgatg cgaaattctg cggacccggg
     2881 cggacc
//
LOCUS       DATMZQ010000105         4346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_734500, whole genome shotgun sequence.
ACCESSION   DATMZQ010000105 DATMZQ010000000
VERSION     DATMZQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734500"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2536)
                     /gene="mdoH"
                     /locus_tag="VN496_03250"
     CDS             complement(65..2536)
                     /gene="mdoH"
                     /locus_tag="VN496_03250"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HXF15991.1"
                     /translation="MELHTAHRMTRGTAEACRRYLERVPFTAPRQHSHAAEADGQNDA
                     PDASADPAEASLEHRLESAVGPDNAQHILTRDRQGRLRLNTAPPLNRTSMIPRRWPPK
                     FGQVQSWWRRPRLDRRADSPDPRGRWHRSASIRRSVLGVVVLAQTIVATDFMTAVLPY
                     HGTQPLEIALLVLFAILFAWVSSGFWTALMGFVMQMFGGDRYVISRTAAPHAPIDAAA
                     RTAIVMPICNEDVVRVFAGLRATYESLEKTGELEHFDFFILSDSADGDNRIAERQAWL
                     GLCRAVGGFGRVFYRWRQHRIKRKSGNVADFCRRWGANYRYMVVLDADSVMSGDCLAT
                     LVRLMEANPGAGIIQTAPRASGRETLYARMQQFATRVYGPLFTAGLHFWQLGESHYWG
                     HNAIIRVAPFIKHCALGRLPGQGTLSGEILSHDFVEAALMRRAGWAVWLAYDLAGSYE
                     EMPPNLTDELKRDRRWCHGNLINFRLFLAEGLHPAHRAVFVTGVMAYGSALLWFFFLL
                     LSTALLAMHTLVVPKYFVAPNQLFPIWPEWHVERALALWSATAAVLFLPKILSVVLVW
                     AHGARAFGGPLRVAISMALEAISSMLLAPVRMLFHSQFVITALAGLRAQWKSPPREDA
                     ETTWSEALQRHGTHSLIGVIWAALVYWLNPAFLWWLLPVVGALMLSIPMSVYSSRISL
                     GRFMRRLGLFVIPEELVSPPELRSLQVHLNNAPPLPGFTDAVVDPLINALTCASANVR
                     RSQPFAIAQARQTLVQEAVTRGPDALSNAQKSVLLGDPSSLSKLHFQVWTSEQAHPGW
                     FAIARVPGRRCTEPAAHTFTPAAAG"
     gene            complement(2509..3996)
                     /locus_tag="VN496_03255"
     CDS             complement(2509..3996)
                     /locus_tag="VN496_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HXF15992.1"
                     /translation="MLFAACALLQPAAFAFSFDDVARSAEQLAAAPYKKPADSLPKEL
                     TSLTQEQYREIRYRPEASWWRDAKLPFELNFFHRGLYFDQQVKVNEVNEQGVSEIKFD
                     PALFDYGASKIDPARMANLGFVGFRVHYPVNNGKVKDEVVSFLGASYFRALGKDQGYG
                     LSARGLAIDTGLPTGEEFPRFVEFWVERPAPTSKELVIYGLMDSRRVAGAYRFVLKPG
                     METVMEIRSRLYLREGVAKLGVAPLTSMFFFGENQKPASDDYRPEVHDSDGLCVHAAN
                     GEWVWRPLVNPKRLLVTSFATASPLGFGLMQRDRDFSHYEDLDARYERRPSAWVEPKG
                     SWGQGRIELVQIPSPDESNDNIVAYWVPDGSPQPKQPWEAEYRILWQKETEAYPPLAW
                     VEQTRRGHGYGKTPENSVGLVVDFEGPIFKKLPPETKLEGIVSGDSNIEILRRLTVRN
                     DVTGGWRMTLLVRRLDAGKPGELRAYLRNGNNTVSETWSYILPTE"
BASE COUNT          936 a         1333 c         1267 g          810 t
ORIGIN      
        1 gggccactcg cggcccttgc ggcattcacg atattcacga tattcacggc attcacggct
       61 cttctcatcc cgcggcggcg ggagtaaacg tgtgcgcagc cggctcggta cagcgacgcc
      121 cgggaacacg cgcgatagca aaccagcccg ggtgtgcctg ctcggaagtc cacacctgaa
      181 aatgcagctt cgacagtgat gacgggtcgc cgagcaaaac tgacttttgc gcattgctca
      241 gcgcatccgg cccgcgagtc acggcctcct gcaccagtgt ttgcctggct tgcgcaatgg
      301 cgaacggctg ggagcgccgc acattcgcgg acgcgcaagt cagcgcattg atcaacgggt
      361 cgactaccgc atccgtaaaa ccaggcaacg gtggcgcatt gttcaaatgc acctgcaagc
      421 tgcgcaattc tggaggactg accagttctt ccggaatcac gaacaggcct agacgccgca
      481 tgaatcggcc aagcgagatg cgactggaat aaaccgacat cggaatggac agcatgagcg
      541 ctcccaccac cggcagcagc caccacagga aagcgggatt gagccagtac accagcgctg
      601 cccagatcac gccaatgagg ctatgcgtgc catggcgttg caaagcttca ctccaggtcg
      661 tctcggcatc ttcgcgcggc ggcgacttcc attgcgcgcg cagacccgcc agcgcggtga
      721 tgacgaactg gctatggaac agcatgcgta ccggcgccag caacatcgaa ctgatcgcct
      781 ccagggccat ggagatcgcg acgcgtagcg gtccaccgaa cgcccgcgca ccgtgtgccc
      841 ataccagaac cacactcagt attttgggaa gaaacagcac cgcagccgtc gcagaccaca
      901 aagcaagtgc cctttcgacg tgccactccg gccagattgg aaacaactgg tttggtgcaa
      961 cgaaatactt cggtacaacc agcgtgtgca tggccagcag tgcggttgaa agcagcagaa
     1021 agaagaacca caacagcgcc gaaccgtacg ccatgacgcc ggtgacgaac acggcccgat
     1081 gcgccggatg caagccttcc gcaagaaaca atcggaaatt gatcaaattg ccatggcacc
     1141 atcggcgatc gcgtttaagt tcatcagtga ggttcggcgg catttcctcg tagctgccag
     1201 ccaggtcgta tgcgagccac acggcccacc ccgcgcggcg catcagcgcg gcttcgacga
     1261 aatcatgcga caggatctcg ccggataatg tgccttgccc cggcaaccgg cccaacgcgc
     1321 agtgcttgat gaacggggcc acacgaatga ttgcgttgtg tccccagtag tgagattcac
     1381 cgagttgcca gaagtgcagt cccgcagtga acagcggccc atacacgcgt gtagcgaact
     1441 gctgcatgcg ggcatacagc gtctcgcgac ctgaggcgcg cggcgcggtc tggatgatgc
     1501 ctgcgccggg gttggcttcc atcaggcgca ccagcgtggc gagacagtcg ccactcatca
     1561 cgctgtcggc atccagcacg accatgtagc gatagtttgc gccccagcgg cggcagaaat
     1621 cagcgacatt gccgctcttg cgcttgatgc ggtgctggcg ccagcggtag aacacccggc
     1681 cgaagccgcc gacggcgcgg cacagtccga gccaggcttg acgctcggca atacgattgt
     1741 ccccatccgc gctgtcgctc aggatgaaga aatcaaaatg ctcaagttca ccggttttct
     1801 ccagtgattc ataagtcgcg cgaagcccgg cgaatacacg aaccacgtct tcattgcaga
     1861 tcggcatgac aatcgcggta cgcgctgccg catcgattgg cgcatgaggc gcggcggtac
     1921 gcgaaatgac gtaacggtca ccgccgaaca tctgcatcac gaatcccatc aatgcagtcc
     1981 agaagccgga cgagacccac gcaaacaaaa tggcgaacag tacgagcagc gcgatctcca
     2041 gcggctgcgt gccgtggtaa ggcaaaacgg cggtcatgaa gtcggtcgct acgatggtct
     2101 gtgccagcac cacgacgccg agcacagagc gccggatgct cgcgctgcga tgccaacggc
     2161 ccctcgggtc aggtgagtcg gcccgccgat cgagccgtgg gcgccgccac caggactgca
     2221 cctgcccgaa ctttggcggc caccgacgcg gaatcatcga agtgcgattc aacggcggtg
     2281 cggtgttaag ccgcagtcgg ccttgccggt cacgtgtcag gatatgttgt gcgttgtcgg
     2341 ggccgacagc gctttccagc cgatgttcaa gggaagcttc ggcgggatcc gctgaagcat
     2401 caggcgcgtc gttttggccg tcggcttccg cggcatgcga atgttgtcgc ggcgcggtga
     2461 acgggactcg ctccagatag cgccggcacg cctcagcggt gccgcgcgtc attctgtggg
     2521 cagtatgtag ctccatgttt cggataccgt gttattacca ttgcgcagat aagcacgcag
     2581 ttcgccgggc ttgccggcat cgagtcggcg caccagcagg gtcatgcgcc acccccctgt
     2641 cacatcgttg cgcactgtca ggcgccgtag gatttcaata ttggaatcgc cagatacgat
     2701 cccttcgagt tttgtctcgg gcggaagttt cttgaaaatt ggcccttcga agtcgaccac
     2761 caggccgacg ctgttctcag gtgttttgcc atatccatga ccgcggcggg tctgctctac
     2821 ccatgccagc ggcggataag cctcagtctc tttttgccag aggatgcggt attcggcttc
     2881 ccagggttgc ttcggttgcg gtgagccgtc cgggacccag taggccacga tgttatcgtt
     2941 cgactcatcc ggcgagggga tttgcaccaa ttcgatcctg ccctggcccc acgaaccttt
     3001 cggttccacc cacgcgcttg gccgccgttc gtaacgcgcg tcgagatctt cataatgtga
     3061 aaaatcgcgg tcgcgctgca tgagcccgaa cccgagcgga ctggccgtgg cgaacgaagt
     3121 cacgagcagg cgcttcggat taaccagcgg gcgccagacc cattcaccgt tcgcagcgtg
     3181 cacgcacaag ccgtctgagt catgcacctc tggccggtaa tcatccgaag cgggcttctg
     3241 gttctcgcca aaaaaaaaca tgctggtcaa cggtgccacg ccaagctttg ccacgccttc
     3301 gcgcaggtac agtcttgacc ggatctccat tacggtttcc atcccgggtt tgagcacgaa
     3361 gcgataagcc ccggcgacac gccgggaatc catcagacca tagatcacca gttccttgga
     3421 cgttggcgcc ggtctttcaa cccagaattc gacgaagcgc ggaaattcct caccggtcgg
     3481 caatccggta tcaattgcca gaccccgcgc agacaacccg taaccttgat ccttgcccaa
     3541 cgcgcgaaaa taactcgcgc ccaggaaaga caccacttcg tccttgacct tgccattgtt
     3601 caccgggtag tgcacgcgaa agccgacgaa tccgagattg gccatgcgtg ccggatcgat
     3661 cttgctcgcg ccgtaatcga acagtgcggg gtcgaatttt atttcgctga cgccttgttc
     3721 attcacctca ttgactttca cctgctggtc gaaatacagg ccgcggtgaa aaaaattgag
     3781 ttcgaaaggc aattttgcat cgcgccacca ggatgcttcc ggccggtaac gaatctcccg
     3841 atactgttcc tgggtcaacg acgtgagttc tttgggcagc gagtccgcgg gcttcttgta
     3901 gggggcggcc gccaactgtt cggcactgcg ggccacatca tcgaaactga aagcaaacgc
     3961 agctggttgc aaaagcgcgc acgccgcaaa cagcagtgcc gcccgaagaa ggacggcccg
     4021 aaggcagtaa gtggctatgc gcacttgagt caccaggaat gggtgggcgg caatgggggg
     4081 ctgagattaa cgaggcgaca ctacgagcgg catgtttagt ttcttatcgc acggcaattg
     4141 caaactcctc ccccttttgg cgcatggcat tcacataggc cagatttggg accgcagcga
     4201 ctgcacaagg ttcggagcga gcaaattgtc ttttgcttgc gtcaaagact ggaccgtgtt
     4261 acgaccgact acgagagttc ggttaaaatg cgcgccaggc cccggtagct cagtggatag
     4321 agcagccccc tcctaagggg caggtc
//
LOCUS       DATMZQ010000106         7967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3884080, whole genome shotgun sequence.
ACCESSION   DATMZQ010000106 DATMZQ010000000
VERSION     DATMZQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3884080"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VN496_03260"
     CDS             <1..1718
                     /locus_tag="VN496_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXF15993.1"
                     /translation="SGWPAEELRIASREGRLEDEGWRLRKDGSRFWASVVITALRDEN
                     GKLLGFSKVTRDLSERKKQEEALRQSEERFRLLIEGVTDYAIFLIDPQGMVTSWNAGA
                     ERIKGYSREEILGKHFSRFYIAKDLESGKPWEELALARRTGRAEDEGWRVRKNGELFW
                     ARVVITALYDTGSRLIGFAKVTQDLTERRHLRDLEKASQHVNEFLGMLAHELRNPLAP
                     IRFAVNVMGKNPITDPTNESMRQMIDRQSAHLAHIVEDLMDISRVSRGKLEIERKSLD
                     MVEVINRAIETATPSITGAHHQLEVDVLPGPLPVSGDIHRLTQILTNLLNNAARYTAQ
                     GGRITVKARAQDSWVVVDVSDNGQGIEPEMIDRIFDMFVQGRSPLQRVGSGLGVGLAL
                     ARRLAELHGGSLIAHSEGANKGSVFTLRAPLDATRSAASDENRRAFAKETSSATAPRR
                     ILVVDDNEDAAVSLGLLLQSLGHQTCVVYSGDEAIKMAIAFRPDVVLLDIGMPGVSGY
                     EVARRLQALKEQGPLRIIAITGWGQEEDKAKSKEAGFDLHLVKPVDAEQLARAVGERN
                     GATVH"
     gene            1825..2373
                     /locus_tag="VN496_03265"
     CDS             1825..2373
                     /locus_tag="VN496_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15994.1"
                     /translation="MLVFLLRIPIWLIGFFLLACAPAGLAQDSVLQSLQSGPAGDFQF
                     APDKSRLTLQISPYVYHVHHGVHNDEPRMIGLEWEPAGSWLEYGATYFRNSFYQDSAY
                     VYVGKRWFAADNEQGAFANVSGGLMYGYRGENADKVPFNHHGVGLAIIPAVGYQYRSF
                     NTQLVFLGTAALMITFGFDLPR"
     gene            2631..3182
                     /locus_tag="VN496_03270"
     CDS             2631..3182
                     /locus_tag="VN496_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15995.1"
                     /translation="MNRFARYAGRLKWLAVFTLGALSAGAVAKDQPVASVAQLAVVAE
                     NSGSVVNPIAANTECRSQDNVKRKGTTDGKPAIRTALFTWAPAALKRSFGGLSDTTVD
                     AWIMEAMRERSRASTMQAVVAGGSLDRQIYAATIAAARAYTNGKYKDAMPSRRYAGQR
                     TGWLRMLRPTPARVVSCGNTIEA"
     gene            3290..3649
                     /locus_tag="VN496_03275"
     CDS             3290..3649
                     /locus_tag="VN496_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15996.1"
                     /translation="MLAIPTTQTLLATGLAMWCAVGTACNALADDSDPADSVAQDIAQ
                     LVQWHSGASAASGSCTGAREVAVLYADSQHISRAFLAMHGAPAVVATELDDADASLAL
                     FERNFLRKSVQSTSSRK"
     gene            3956..4531
                     /locus_tag="VN496_03280"
     CDS             3956..4531
                     /locus_tag="VN496_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF15997.1"
                     /translation="MDIADRKRLVRAVLFLVISTGDVASADEGDPASEMPGFSTSKDT
                     DSWFNLRALDINVYGLSYHPDRQTVHEKNLDNQVNPGLALHYQLHETARGITFVEAGA
                     YEDSGSNWAKFAGLGYQFKFGNWKIGGAVAAVNSATYNRGTTFVGMIPLVTWDLGRIK
                     LNGTYFPKIANYNEIDAFGFYLSIPLGAWMH"
     gene            complement(4571..5323)
                     /locus_tag="VN496_03285"
     CDS             complement(4571..5323)
                     /locus_tag="VN496_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXF15998.1"
                     /translation="MPSDPHIAAHPIPEQSGAAERRAFIANNLLSALPRKDYRRMLTG
                     LEPVNLSFGEVLYQPGEAISHVYFPGIALVSLMTLVDGRLTLEVGVVGCEGMVGLPVA
                     LGIGVSPFRALVQGAGGALRMKSNYFVKELRQSIALQRALQRYTHALMAQFALTAACN
                     RFHVVEERLARWLLMTRDRVQSTDFRLTQEFLSHMLGVRRVGVTKAAGHLQQRRLIEY
                     SRGNIRIVDQAGLEAACCSCYQASRQWRSQSS"
     gene            complement(5348..6079)
                     /locus_tag="VN496_03290"
     CDS             complement(5348..6079)
                     /locus_tag="VN496_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXF15999.1"
                     /translation="MLTPDLPRRNHLLAALSEDDYIRLLPHLEGIELTKGQVVAESGV
                     SQGYVYFPTSSLFALLWPMEDGAQIQTGLVGNEGVAGIGLCFGTLGIPGRTVVQSRGY
                     AYRLAAAVLKAEFDRRGLLQHLLLRFTQVLLIQMAQSAVCREHHSIEQQLCRWLLLTL
                     DGSPSDPLAQANDSIADMPDGRRAGMTEAANQLKAGEVLQYRDGKIALDRPKLELRVC
                     DCYSIGKVELDGLFPRKPVSAHQPA"
     gene            complement(6206..7411)
                     /gene="gspF"
                     /locus_tag="VN496_03295"
     CDS             complement(6206..7411)
                     /gene="gspF"
                     /locus_tag="VN496_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HXF16000.1"
                     /translation="MEAFQYQALDATGRMVSGVVQADTARQARTQLRARGLLPSTIGP
                     VHARERARQHWARGISAAELSLVTRQMATLLGSGLTMEQSLNALIEAATEPVTREVLT
                     GVKTEVTAGLSLANALGAYEKSFPDFYRALVHGGEESGALPIVLQHLADYLDSRQAMK
                     QKTGLALLYPILVTIVALSIVTGLLVYVVPQVVQVFQQSRQNLPLLTRALIGLSDFLR
                     STWPYIAGFVVTAALGARWLLRRDAERRRWHALLLRLPWLGALIRGVNTSRFASTLAI
                     LVGGGVPLLSALASGARVMTNMVMQEAVERTIERVREGAGLARALGETRAFPPLLIHL
                     IASGEVSGKLEQMLERAAHLESQALERRLAVFLTLLEPVMILVMGGVVLMIVLAILLP
                     IIEINQLVH"
     gene            complement(7430..>7967)
                     /locus_tag="VN496_03300"
     CDS             complement(7430..>7967)
                     /locus_tag="VN496_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXF16001.1"
                     /translation="QDPDVVMIGEIRDLETAQIAVQASLTGHLVFATLHTNDAVSAVT
                     RLVDMGVEPFLLASSLIGVIAQRLVRRLCMECRKPFAANETQLATLGLAPTNGTLYSA
                     QGCPACNRSGYKGRTGIYEMLTVDDELRRLIHDQSSEQILRTHVISHGMRTLREDGMR
                     WAGQGIISLEEVVRVTRE"
BASE COUNT         1688 a         2294 c         2414 g         1571 t
ORIGIN      
        1 acagcggctg gccggcagag gagctcagaa tcgcatcccg cgagggaaga ttggaggacg
       61 agggctggcg gctccgcaag gatggctcgc gattttgggc gagtgtcgtg attaccgcgt
      121 tacgcgatga aaatggcaag ctgctcggat tttccaaggt cacacgggac ctgagcgagc
      181 gaaagaaaca agaagaagca ctgcgccaaa gcgaagagcg gttcaggttg ctcatcgaag
      241 gcgtaacgga ctatgcgata ttcctgatcg atccccaggg catggtcacc agctggaacg
      301 cgggtgcgga acgcatcaaa ggctacagtc gcgaagaaat cctcggcaag cacttctccc
      361 gcttttatat tgcaaaggat ctggagtcgg gaaagccatg ggaggaactc gctctcgcgc
      421 gccgaaccgg ccgcgcagag gatgaaggct ggcgcgtccg gaaaaatggt gagcttttct
      481 gggcgagggt tgtgataacc gctctgtatg acaccgggag ccgcctgatt ggattcgcca
      541 aggtcacaca agacctgacg gaacgacggc atttgcgtga tctggaaaaa gcctcgcagc
      601 atgtaaacga gttcctcggg atgctggcac atgagttacg aaacccgctt gcgccgattc
      661 gctttgcggt gaacgtgatg gggaaaaacc cgatcaccga tccgactaac gagagcatgc
      721 gccagatgat cgaccggcag agcgcgcacc tggcccatat cgtagaagat ttgatggata
      781 tatcgcgtgt gagccgaggc aagctcgaga tcgagcggaa aagcctggac atggtggaag
      841 tgatcaatcg ggccatcgaa acagcgacac cttcaatcac gggtgcgcac caccagcttg
      901 aggttgatgt cttgcctggc ccgctgccgg tctccggaga cattcaccgt ttgacgcaga
      961 ttctcactaa cctgctgaac aacgcagctc ggtataccgc ccaaggtggg aggattaccg
     1021 tgaaggcgcg agcgcaagac agctgggtgg tcgtggacgt tagtgataac gggcagggta
     1081 tcgagcccga gatgatcgac cgcatttttg atatgttcgt gcaaggccgt tcacctttgc
     1141 agcgggtcgg cagcgggctg ggtgtgggcc ttgcgctggc acgacggctc gcggaactgc
     1201 atggcggttc gctgatcgcg cacagcgaag gcgcaaacaa aggtagcgtc ttcacattgc
     1261 gcgcgccgct ggacgctaca cggtccgccg caagtgatga gaacagacgg gcctttgcca
     1321 aggagacttc ttccgccacc gcacctcgcc gcatcctggt agtcgacgac aacgaggacg
     1381 cggccgtttc gctggggctg ctgctgcagt cgcttggaca ccaaacctgt gtcgtctaca
     1441 gtggagacga ggctatcaag atggccattg cttttcgtcc cgatgtcgtg ttgcttgaca
     1501 tcggcatgcc cggcgtcagc ggttacgagg tggcgcggcg actacaagcg ttaaaagagc
     1561 aagggccatt gcggatcatt gcaatcacgg gctggggcca ggaggaagac aaagccaagt
     1621 ccaaagaagc gggcttcgat cttcatctgg tcaagcctgt cgatgcggag cagttggcgc
     1681 gagcagtcgg cgaaaggaac ggcgcaaccg tgcactaagc aataccgcgt gtagatgcga
     1741 cgctgcatgt gagcgcgcct ccgattgcat gggctattgg ggaggcgttt tagagtccct
     1801 tatttccttt ccatctctgc acagatgctg gtttttttgt tacgcattcc catatggctg
     1861 atcggatttt tcttgctagc gtgcgcacca gcaggcctgg cgcaggattc ggtcctccaa
     1921 tcgttgcaaa gcgggccggc tggcgatttt cagttcgcgc ccgacaagag ccgactgacg
     1981 ctgcagatca gtccctatgt gtaccacgtc caccacggcg tgcataacga tgagccccga
     2041 atgatcggcc tggagtggga gccggccgga tcctggctgg aatacggggc tacatacttc
     2101 aggaattcct tttaccagga cagcgcctat gtttatgtcg gcaagcgctg gttcgctgca
     2161 gacaacgaac agggcgcttt cgcgaacgta agtggcggtc tcatgtatgg ctaccgcggc
     2221 gaaaatgcag acaaggtgcc tttcaatcat cacggtgtgg ggctggcaat cattcccgca
     2281 gtcggttacc agtatcgttc tttcaacacc cagcttgttt tcctgggcac cgcggctctc
     2341 atgattacat tcggttttga tttgcctcga taatgccatg ctccatgggg tggacagcag
     2401 cggtctgctt ccggctcgcg aaaatcttct catccatgcg tatggctacg ttgcatcaat
     2461 atgacatccg ggttttgtga tgggggccga tagagccacg cggcaactcc caagccggca
     2521 attaacggcg ctgtgtgcgt tggcgcacag aaaagtgcca ggtcacagtg cagtctgcat
     2581 gacgggcaca tgtccttcgc caaattgcca atgcctggga gggcgagcaa atgaacagat
     2641 tcgcgcgcta tgccggacga ctgaagtggc ttgcagtttt tacgcttggc gcgctcagcg
     2701 cgggcgcagt cgccaaagac caacctgttg cgtcagtcgc ccagctcgct gtggtcgcgg
     2761 aaaacagcgg ctccgtagtt aatccgatcg ccgcaaatac ggaatgccgg tcgcaggaca
     2821 acgtcaagcg caaaggaaca acggacggca agccagcgat ccggaccgca cttttcacct
     2881 gggccccagc cgcgctgaag cgctcgttcg gcggcctttc tgatacgact gtcgatgcct
     2941 ggatcatgga agcaatgcgg gaacggtcaa gggcttcaac catgcaagca gtagtagccg
     3001 gcggatcgct ggatcgacaa atctacgctg cgactattgc tgcagcgagg gcgtacacga
     3061 acggcaaata caaagacgcg atgccctccc gacgctacgc cggccagaga accggttggt
     3121 tgcgcatgtt gcgtccgacg cctgcaaggg tcgtcagctg cggcaataca atcgaagcgt
     3181 aatcgtcacg gcaaacacga atttgcgtgc cccgcgtgca tatgaaaagg atggcaaaac
     3241 ggtgcccggc cggccgtcga tgcatgaggt ggcggggtac acgaaaacca tgctagccat
     3301 accaaccacc caaacgctgc tagcgaccgg cctggcgatg tggtgcgccg tggggactgc
     3361 atgcaatgca ctggctgacg attcagaccc agcagattct gtggcacagg acattgcaca
     3421 attggtgcag tggcattctg gagcaagcgc tgcaagtggt tcatgcaccg gcgctcgcga
     3481 ggtcgccgtg ctttacgccg atagtcagca catttctcgg gcattcctcg cgatgcacgg
     3541 cgcacccgct gtggtggcca cagagctgga cgacgcggat gcttcgctcg cgttgttcga
     3601 gcggaatttc ttgcgcaaat ctgtacagag cacctcctct cgcaagtgac gtaaaccggc
     3661 agcgggtaga ctgatacccg tatacctcaa cgcggttccg cgcaggcgaa cgtcgccaca
     3721 atgagcatcc agcgcgtgtt cggctggttt gcaattcttc ttttcccggt tttccagtag
     3781 ttactttttt cgtccaaatg tgcggcaccg cactgacgaa aattgcgcgt tgtgcttgca
     3841 gggcggcacg cgtaagctgc gcgcgctcac agattatcaa gtaaacaact gagcagtctt
     3901 cgcggaattg tatccgtcgc atgggtgcgg agatgctcgg caagcagggg gaatattgga
     3961 tatcgccgat cggaagcggc tggtccgcgc agttttattt cttgtaattt ctaccggcga
     4021 cgttgcgagc gccgatgaag gcgacccggc gagcgagatg cccgggtttt cgacttccaa
     4081 ggacactgat tcctggttca atctacgcgc actggatatc aacgtatacg gactctcgta
     4141 tcatcccgac cgtcaaacgg ttcacgaaaa aaatctggat aaccaggtca atccgggcct
     4201 cgctcttcat taccagctgc atgagaccgc gcgaggcatt acttttgtgg aggcgggcgc
     4261 atatgaggat tcaggcagca actgggcaaa atttgccgga ttgggctatc aattcaagtt
     4321 cgggaattgg aagatcggcg gcgccgtggc tgccgtcaac agtgcaacct acaatcgcgg
     4381 cacgacgttt gtcggcatga ttccgctggt aacctgggat ctcggacgca tcaaactgaa
     4441 cggaacttac ttccccaaga ttgccaacta taacgagatc gatgccttcg gattctactt
     4501 gagcattccg cttggcgcgt ggatgcattg atcacgtcgt tgaaacgttg aaaacggcac
     4561 gccgatcggg ttacgacgat tgggagcgcc attgccggct cgcctgatag cacgaacagc
     4621 aggccgcctc aaggccggct tgatccacga tccgtatatt tccgcggctg tactcgatca
     4681 gtctgcgctg ctgcaagtgt ccagccgctt tggtaacacc aacgcgccgt acgccaagca
     4741 tgtgggaaag aaactcctgc gtgagcctga aatcggtgga ctgaacccgg tcccgtgtca
     4801 tgagtaacca gcgtgcgagg cgttcctcga ctacgtggaa ccggttgcat gcggcggtca
     4861 gggcaaattg ggccattaac gcatgggtgt agcgctggag cgcgcgctgc agagcgatac
     4921 tttggcgcag ctctttgacg aagtaatttg atttcatgcg caaggctccg ccggctccct
     4981 gcaccagcgc ccgaaacggt gatacgccaa tgccgagcgc gacaggcaaa ccaaccatgc
     5041 cttcgcaccc gacaacgcct acttccagcg tgagacggcc atccacgagt gtcatcagtg
     5101 atacaagcgc aatgccaggg aagtagacat gactgatcgc ctctcccggc tggtacagca
     5161 cctcgccaaa ggagaggttc actggctcaa ggccagtcaa catgcgccgg taatctttac
     5221 gcggaagagc gctcagcaag ttgttcgcaa tgaacgcgcg ccgttccgct gccccgcttt
     5281 gctccgggat gggatgggca gcgatgtgcg ggtcggacgg catggccaag gtgtgggaag
     5341 gccacgctca cgcaggctga tgtgccgaga cgggcttgcg cggaaacaag ccgtccaact
     5401 caactttgcc gatcgaatag cagtcacaca cacgaagctc gagttttggc cggtcgagcg
     5461 ctattttccc gtcacgatat tggaggacct ctcccgcttt cagctggttg gccgcctcgg
     5521 tcatgccggc ccggcggccg tccggcatgt cggcaatgga atcatttgcc tgcgcaagcg
     5581 ggtctgatgg cgagccatcg agggtcagca acagccaacg gcataactgc tgctcgatgg
     5641 aatgatgctc ccggcacact gcactctgtg ccatttggat gagcagtact tgggtaaaac
     5701 gcagcagcag gtgctgtagc aggccgcgcc ggtcgaattc agccttgagc accgctgccg
     5761 caaggcgata ggcatagccc cggctttgca ccaccgtacg tcccggaatg ccaagcgtgc
     5821 cgaagcagag cccgatgcct gcgacaccct cgttgcccac cagcccggtc tgaatctgcg
     5881 cgccatcttc catcggccac aacagggcaa acaggctgct cgtgggaaag tatacgtagc
     5941 cttggctaac gcctgactcg gccacaacct ggccctttgt gagttcgatg ccttcgaggt
     6001 gtggcagcag gcggatgtaa tcgtcttccg agagggcggc cagcagatgg tttcgtctgg
     6061 gaaggtctgg tgtcaacatg gaaagcctta ccgtcgagta gcagtcatgg gtggaccggt
     6121 gacggtagaa gcgtagttca atgcaaaacg tccgtatgtt cgatagcgca cggaacgcgc
     6181 cagggcgatc cggcgcagca gccggtcagt gcaccagctg gtttatttca atgatcggaa
     6241 gcaggatagc gagcacgatc atgaggacta cgccgcccat gaccaggatc atgaccggtt
     6301 cgagcagtgt caggaatacc gccagccgac gctcgagtgc ctgcgattcc aggtgcgcgg
     6361 cgcgttccag catctgttcg agtttgccgc tgacttcgcc gctcgcgatt agatggatga
     6421 gcagaggcgg gaaggcacgc gtttcaccta acgcgcgcgc caggccggca ccttcgcgaa
     6481 cccgttcgat agtgcgttcc acggcttctt gcatgaccat gttcgtcatg acgcgcgcgc
     6541 cggatgcgag tgccgagagc agcggcacgc cgccgcccac cagtatcgcg agcgtgctgg
     6601 cgaagcgcga ggtgttcaca ccgcgaatca gcgccccaag ccacggcagg cgtaacagca
     6661 gggcatgcca gcggcgccgt tccgcatccc ggcgcagcag ccagcgcgca cccagggcag
     6721 cagtgacgac gaagccggcg atatagggcc aggtactgcg cagaaagtca cttaaaccga
     6781 tcagcgcgcg cgtcagcagc ggaagattct ggcgcgattg ctgaaacacc tgcaccacct
     6841 gcggtacgac atacaccagc agcccggtga cgatggaaag cgccacgatg gtcacgagaa
     6901 tcggatacag cagcgccaac ccggttttct gtttcatcgc ctggcgggag tccagatagt
     6961 ccgccaggtg ctgcagtacg atcggcagtg cacctgattc ttcgcccccg tgcacgagcg
     7021 cgcggtagaa gtcgggaaag cttttttcgt aggcacccaa agcgttggct aacgacaggc
     7081 ccgcggtgac ctcggttttc acgcctgtga gcacttcgcg tgtcaccggt tcggtcgccg
     7141 cttcgatcag cgcgttcagt gactgctcca tggtgagacc ggagccgagc agcgtcgcca
     7201 tctggcgagt cacgagactc agttcggcag ccgagatgcc gcgcgcccag tgctggcgcg
     7261 cccgctcccg cgcgtgcacc ggaccgattg ttgaaggcag cagacctcgt gcgcgcaatt
     7321 gcgtacgcgc ctggcgtgcc gtgtcagcct gcaccacccc tgacaccatg cggccagttg
     7381 catcgagcgc ctgatactgg aatgcttcca tcgatcaagc cactgcccgc tactcgcgcg
     7441 tgacgcgcac gacttcctcg aggctgatga tgccttgtcc ggcccagcgc attccgtctt
     7501 cacgcaacgt gcgcatgccg tgtgaaatca cgtgcgtacg caggatttgc tcggaacttt
     7561 ggtcatggat cagccggcgc aattcatcat cgaccgtcag catttcataa atgccggtgc
     7621 gtcccttgta gcccgagcga ttgcatgccg ggcagccttg cgcggagtac agcgtgccat
     7681 tggtgggcgc caatcccagc gtcgcgagtt gcgtttcatt ggcggcaaaa ggcttgcggc
     7741 attccatgca cagccggcgc accaggcgct gcgcgatcac gccgatcagg ctggacgcga
     7801 gcaggaacgg ttcaacaccc atgtcgacca gacgcgttac ggcgcttacg gcatcgttcg
     7861 tgtgcaacgt cgcaaacacc aggtgcccgg tcaagctggc ctgtaccgcg atctgtgccg
     7921 tctccaggtc gcgaatttct ccgatcatga cgacgtccgg atcctgg
//
LOCUS       DATMZQ010000107         6651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4531737, whole genome shotgun sequence.
ACCESSION   DATMZQ010000107 DATMZQ010000000
VERSION     DATMZQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4531737"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VN496_03305"
     CDS             <1..363
                     /locus_tag="VN496_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXF16002.1"
                     /translation="GSNWEAALAAAHYKLSNLTAIIDFNKICQSGRVQEVIGIDPLAD
                     KWRAFGWEVREIDGHDMSQVVDTLDAVPLDKHRPTALLAHTVKGKGVSFAENTYVWHS
                     NSVNDEIYAKALAELNMP"
     gene            360..1331
                     /locus_tag="VN496_03310"
     CDS             360..1331
                     /locus_tag="VN496_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXF16003.1"
                     /translation="MNAPDTAATAWRSKSANQTRLAYGQALLEIAAREPRAVVVSADT
                     QALLGIRTYIERFRERFVELGIAEQNVIGAAAGMATTGLRPYVCAYAPFISARSMEQV
                     RNDVAYANQRVVIGAAASGISLGVAGGTHHALEDLALMRSLPNMTVIVPADVNEAYRA
                     TLATLDIDGPVYLRLGGRSEEPPVGDPEAPFRLGKAVQLRSGMDVTVIACGALVEMAL
                     RASDMLKGQGIGARVLNMHTIKPLDRDAILAAAAQTRGIVTAEEHHLTGGLGGAVAEL
                     LAVEHPTRMRMVGMPDAFAMVGPTGPLRTKYGMSTEAIAEACHALLG"
     gene            1444..1767
                     /locus_tag="VN496_03315"
     CDS             1444..1767
                     /locus_tag="VN496_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXF16004.1"
                     /translation="MLLDVRTYTCRPGTIKKHLALYEKLGKGPQTKHLGQPFAYLVTE
                     TGNVNQYIHIWAYEDAADRAKRRAAMMADPDWIAFMDESAKLGALEAQENRLMTPVSF
                     FPLKR"
     gene            complement(1850..2140)
                     /locus_tag="VN496_03320"
     CDS             complement(1850..2140)
                     /locus_tag="VN496_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16005.1"
                     /translation="MNTPIPPIPPRRRLQELLAIPERQRTDEQWDEINELEIKLASAN
                     REDAPDPSMRRDGSSNNNNRRNRPPGGGQQGKRPPRKFHNKKPGGPGGGPDR"
     gene            2190..3509
                     /gene="dinB"
                     /locus_tag="VN496_03325"
     CDS             2190..3509
                     /gene="dinB"
                     /locus_tag="VN496_03325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXF16006.1"
                     /translation="MAASPSFDPDKKFSEIMPCRWIAHLDMDAFYASVELLRYPALRG
                     RAVVVGGRRAHQPVLQPDGSHHFFELRTYAGRGVVTTATYEARALGVHSAMGLMKAAA
                     LAPDAVLLPADFDEYRKYSRLFKAAVAEIAPHIEDRGIDEIYVDLTDIPGAQDASGDD
                     AFAGVRAIAERIKARVYLATGLSCSMGVTPNKLLSKISSDLDKPNGLTILTHDDLASR
                     IWPLPAKRINGIGPKANAKLEALGIRTIGQVASADPHLLIEQFGENYGAWLHQAAHAR
                     DERPVVTFSEPKSISRETTFERDLHARDDRAALGSILTRLCEQLAADLQRKGYVARTI
                     GVKLRYDDFRIETRELTLETHIQDAVTIRQTAGRCLKKTTLDRRLRLLGVRAGTLCRT
                     DALATASKEADGPQRDQLALPMFEASRTPGELTAPKPPRSFPGGRNE"
     gene            3506..4372
                     /locus_tag="VN496_03330"
     CDS             3506..4372
                     /locus_tag="VN496_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXF16007.1"
                     /translation="MIAFFRHAAEVVHHPLRFGLQVINAFRANQGLLLAGALAYYALL
                     SMVPLLILIVIALSHFLDQSELLGMVGRALEWVAPGQSRAVVNELATFLEAHVALSWL
                     LIVTMLLFSSLAFTVLEGAMSVIFMHRLAVRKRRFVVSALIPFGYIFFLGLALLVGTL
                     VQASLESVGKETIVVFGHAWSLGGVSGFLLYIVGVVIEMLLIASVYLVMPFGRLSLLH
                     ALIGGVTAGILWEAIRHSLVWYFSTISQVGVVYGSFTSVIVVLLSFEMGATVLLLGAQ
                     VIAEYERLERSG"
     gene            4441..5448
                     /locus_tag="VN496_03335"
     CDS             4441..5448
                     /locus_tag="VN496_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HXF16008.1"
                     /translation="MTILLAPMEGLLDFVLRDIFTRVGGIDRCVSEFIRVTGTLLPDR
                     VFTRIVPELNNGGRTPAGVPVRAQLMGSDPVCLAENAAKLAMLGPAGIDLNFGCPAKG
                     VNRHRGGAVLLDEPELIAQIVSAVRRAVPASMPVSAKMRLGYNDTSRALDCALAIAEG
                     GANELVVHARTKTDGYRPPAYWNRIRQIRAVVSIPVIANGEIWTAEDASQCRMESGCN
                     ALMLGRGIVADPGLALAIRRLDSHSAESSHANSVVNHGDFGWDALLPFVAHFWHIVGL
                     HIAAHHRAGRLKQWLNFLRRRHPQAQEAYAAVRTINDPLLITKELFAHTGAVDEIPLA
                     V"
     gene            5426..5875
                     /locus_tag="VN496_03340"
     CDS             5426..5875
                     /locus_tag="VN496_03340"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HXF16009.1"
                     /translation="MKYRLRFDALIAACGLVAALFVDTTLSAADLVLRPGLYEVEVRI
                     SLPNVGEAAPANLVTRCITPDDVRSGGAFFILSDNPLRQCGVLDYQTTSSTVVYRIEC
                     AGPNRGSAVATFDIKETAYRGTIKMNMGGKNMIMSETQKGKRTGDCQ"
     gene            6070..6609
                     /locus_tag="VN496_03345"
     CDS             6070..6609
                     /locus_tag="VN496_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HXF16010.1"
                     /translation="MGSLFESLSAKTANGLVNVIIDTPKGSRNKFKFDEQVQCFRLSR
                     ILPLGACFPYDFGGIPGTAAEDGDPLDVLVIAQEGSFVGCLLTAKLIGGVRAEQTEKG
                     RTIRNDRLLAVPVTNANPAEFSHIDQLPSARVAEIEHFFVSYNQAHGRQFRPTGHAGP
                     DEADRVLAAAIQRFEESRE"
BASE COUNT         1279 a         1968 c         2030 g         1374 t
ORIGIN      
        1 ggctccaact gggaagcggc gctggccgcc gcgcactaca agttatctaa cctgactgcc
       61 atcattgact tcaacaaaat ctgccagagc ggccgcgtgc aagaagtcat tggcatcgac
      121 cctctcgcag ataaatggcg agcgtttggc tgggaggtgc gcgaaatcga tggacacgac
      181 atgtcgcaag tcgtggatac actcgatgca gtaccgctgg acaagcatcg ccccacggcg
      241 ttgttggctc acacggtcaa aggcaaaggc gtcagctttg cggagaacac gtatgtgtgg
      301 cacagcaatt cagtcaatga tgaaatttat gccaaggcac tggctgaatt gaatatgcca
      361 tgaacgcacc cgacactgcg gccacggcct ggcgttccaa atcagccaat cagacacgcc
      421 ttgcgtacgg ccaggcattg ctggagatcg cagcacgcga gccacgtgcc gtggtcgtat
      481 ctgccgatac acaggctctg ctcggcattc gcacctacat cgaacgcttt cgcgaacgct
      541 tcgtggaatt gggcattgct gaacagaatg tcatcggcgc ggcggccgga atggctacca
      601 ccgggcttcg gccgtacgtg tgtgcttacg cgcctttcat ctcggcgcgc agcatggagc
      661 aagtgcgcaa cgatgtggcg tacgcgaatc agcgtgtcgt gatcggggcg gccgcaagcg
      721 gcatttcgct gggcgtagcc ggcggcacgc accatgcgct ggaagatctt gcactcatgc
      781 gcagcctccc gaacatgacg gtgattgttc cggccgatgt gaacgaagcg taccgggcta
      841 cgctggcgac gctcgacatc gatggcccgg tctacctgcg acttggtggc cgctctgaag
      901 agccgcctgt cggcgacccg gaagcgcctt ttcgcttggg aaaagcggta cagctgcgca
      961 gtggcatgga cgtcacggta attgcatgcg gcgcactggt ggaaatggct ctgcgcgcgt
     1021 cagacatgtt aaaagggcaa ggcattggcg cgcgcgtatt gaatatgcat accatcaagc
     1081 cgctcgatcg cgacgcgatc ctggctgctg ccgcgcaaac acgcggcatc gtgaccgccg
     1141 aagaacacca tttgaccggc ggtctcggtg gcgctgtggc ggaattgctg gcagtcgaac
     1201 atcccactcg catgcgcatg gttggcatgc cggacgcctt tgcgatggtt ggccccaccg
     1261 gcccgttgcg cacgaaatac ggcatgagca ccgaagcgat cgccgaggca tgccacgcgc
     1321 tgcttggttg agcaactgtc ggatcgctct ccgtcttatc tctttcccgt tttccgtcgg
     1381 ccgattaaac tcgcgcgaca cgcgcatcgc gattgcctaa cccaaaaacc caccggagga
     1441 accatgctgc tcgacgtccg cacgtacacg tgccgccccg gcaccatcaa gaaacatcta
     1501 gcgctgtacg agaaacttgg caagggaccg cagaccaaac acctcggcca gccgttcgct
     1561 taccttgtaa ccgagactgg caacgtgaat cagtacattc acatctgggc atatgaagat
     1621 gcggccgacc gggcgaagcg gcgcgccgcg atgatggccg atccggactg gattgcattc
     1681 atggacgaaa gtgcgaagct gggcgcactc gaagcacagg aaaaccggct catgactccg
     1741 gtatcgttct tcccgctgaa gcgatagacc gcttcagcgc tcgccaaggt caggagccag
     1801 gacgaaccaa cacagcattc gccagctttg cagcaagacg ctgctggtgc tagcgatccg
     1861 gtccgccgcc cggacccccg ggcttcttgt tgtggaactt ccgcggtggc cttttgcctt
     1921 gctggccacc gcccggcggg cgatttcgcc gattattgtt gttggaagag ccgtcgcgac
     1981 gcatgctggg atccggcgca tcctcccgat tggcggaggc gagcttgatc tccagttcat
     2041 tgatttcatc ccactgctcg tcggttctct ggcgctccgg aatggcgagg agctcctgca
     2101 ggcggcggcg gggcggtatg ggtggtatcg gtgtattcat ggcgttattc tcgcatcctt
     2161 ccaaggcttt agcatagtct ggcgctggaa tggccgcatc cccgagcttt gatcctgaca
     2221 aaaaatttag tgaaatcatg ccttgccgct ggattgctca tctggacatg gatgccttct
     2281 acgcgtccgt ggagctgctg cgttacccgg cgctacgcgg tcgtgccgtc gtagtcggtg
     2341 gacggcgagc gcaccagccg gtcttgcagc cggacggctc gcatcatttt ttcgagttgc
     2401 gcacctatgc cgggcgcgga gttgtcacaa cggcgaccta tgaggcgcgc gcgctcggtg
     2461 tccattccgc gatgggattg atgaaggcag cggcgctcgc gccagatgcg gtactgctgc
     2521 cggccgattt cgacgagtac cgcaaatatt cgcggctgtt caaagccgcc gtggcggaga
     2581 tcgctccgca catagaagat cgcggcatcg atgaaatcta cgtcgacctt accgacattc
     2641 ccggcgcgca ggacgcctcg ggcgacgatg catttgcggg cgtgcgcgcg atcgcagaac
     2701 gcatcaaggc gcgggtgtac ctggcgacag ggctcagctg ttcaatgggc gtcacaccca
     2761 acaagctgct ttccaaaatc agttccgatc tggacaagcc caatggcctg acgatcctga
     2821 cacacgatga ccttgcttcg cggatttggc cgctgccggc caaacgcatc aacggcatcg
     2881 ggccgaaagc aaatgcaaaa ctcgaagcgc ttggcattcg cactatcggg caggtcgcga
     2941 gcgccgatcc gcatttgctt atcgagcaat tcggagaaaa ctatggagca tggctgcacc
     3001 aggccgcgca cgcgcgggac gagcgcccgg tggtcacgtt cagcgaaccg aagtccatca
     3061 gccgtgaaac gacgttcgag cgcgatctgc acgcgcgcga cgatcgtgcc gcgctgggtt
     3121 cgatcctcac gcgtctttgc gagcagctcg ctgccgatct gcagaggaag ggttatgttg
     3181 ccaggaccat cggcgtcaaa ctgcgttacg acgattttcg catcgagacg cgcgagctga
     3241 cgctggaaac ccatatccag gatgcggtga ccattcgcca gaccgccggc cgctgcctca
     3301 aaaaaaccac gctcgatcgg cgcctgcgcc tgctgggagt cagagcaggc accctgtgca
     3361 ggacggatgc cctggcgacc gcgtcgaagg aggcagacgg cccgcaacgc gatcaacttg
     3421 ccctgccgat gttcgaagca agccgtacac cgggtgagct gacggcgccc aaaccgcccc
     3481 gctcatttcc cggcggccgc aacgaatgat tgcattcttc cggcacgccg ctgaggtagt
     3541 acaccatccg ctgcgtttcg gattgcaggt catcaacgcg tttcgcgcca accagggact
     3601 gctactggcc ggtgcgctgg cgtactacgc actgttgtcc atggtgccgc tgctgattct
     3661 gatcgtcatc gcgctatcgc attttctcga ccaaagcgag ctgctgggca tggtgggacg
     3721 cgcactcgaa tgggtcgcac cgggacaatc gagggccgtc gtcaatgaac tggcgacgtt
     3781 cctcgaggct cacgtcgcgc tgagttggct gctgatcgta acgatgttgc tgttcagctc
     3841 gctggcgttc acggtgctcg agggcgcgat gtcggtgatc ttcatgcatc gtctcgccgt
     3901 gcgcaaacgc cgttttgtgg tttctgcatt gattccgttc ggctacattt ttttcctggg
     3961 actcgcgctc ctggtgggaa cactggtcca ggccagtctc gagtcagtcg gcaaggaaac
     4021 catcgtcgtg tttggacacg catggtcgct cggcggggtt tccggttttt tgctctatat
     4081 cgtcggcgtc gtaattgaaa tgttgctcat tgcatcggtc tacctggtca tgcctttcgg
     4141 ccggctgtcg ctgctgcacg cattgatcgg cggggtgacg gccggcatcc tgtgggaagc
     4201 aatccgccat tcgctggtat ggtatttcag cacgatctcg caagtgggcg tcgtatacgg
     4261 ttcattcacc agcgtgatcg tggtgctgct gagtttcgag atgggcgcca ccgtgctatt
     4321 gctcggtgcg caggttatcg cggaatacga gcgcctggaa cgtagtggat aatctgggtc
     4381 cgacccaaag ccggccgcgt gtcaccagcc gctagcattc gcggctagta ttatgacctt
     4441 atgactattc tgctcgcgcc catggagggc ctgcttgatt tcgtattgcg cgatatcttc
     4501 acgcgcgtcg gtggcatcga ccgctgcgtg tcggaattca tccgcgtcac cggcaccttg
     4561 ctgccggacc gtgttttcac gcgcatcgtg cccgaattga acaacggcgg ccgcacgccc
     4621 gccggcgtgc cggtgcgggc acagctgatg ggatccgacc cggtgtgtct ggcggaaaat
     4681 gccgcaaaac ttgccatgct gggtccggcc ggcatcgatt tgaattttgg ctgcccggcg
     4741 aaaggcgtca atcgccatcg cggtggcgcg gtactgctcg atgagcccga gctgattgcg
     4801 cagatcgttt ctgccgtgcg tcgcgccgtg ccggcatcca tgcccgtttc ggcgaaaatg
     4861 cgactgggct acaacgacac gtcacgcgcg ctggattgcg cgctcgccat tgcggaaggc
     4921 ggcgccaatg agctggtggt acacgctcgc acaaaaaccg acggctatcg gccgcccgct
     4981 tattggaatc gcatccggca aattcgtgca gtggtcagta tcccggtcat tgcgaatggc
     5041 gaaatctgga ctgccgaaga tgcgtcgcaa tgccggatgg aatcaggatg caatgcgctg
     5101 atgctgggcc ggggcatcgt ggcggacccg gggttggcgc tggcgatcag gaggctggat
     5161 tcgcattcag ccgagagctc gcacgccaac tcagttgtca atcacggcga tttcggttgg
     5221 gatgcattgt taccgttcgt cgcgcacttt tggcacatcg ttggcttgca catcgccgcg
     5281 catcaccgtg ccggccggct caagcaatgg ctcaattttc tgcggcgccg ccacccgcaa
     5341 gcgcaggaag cctatgcggc ggtgcgcacc atcaatgatc ctctgctcat cacgaaggaa
     5401 ctatttgcgc acaccggtgc agtcgatgaa ataccgcttg cggtttgatg cgttgatcgc
     5461 agcgtgcggg ttggttgccg cgcttttcgt agacacgact ttgagcgcgg cagatcttgt
     5521 cctgcgccct gggttgtatg aagtggaagt gcgcatcagc ctgccaaatg taggcgaagc
     5581 tgcgccagcg aatctggtga cccgctgcat tacaccggat gacgtccgtt caggtggagc
     5641 gttttttatc ctgagcgaca atccgctcag gcagtgtggc gtgctggatt accagaccac
     5701 ttcatccaca gtcgtctatc gcatcgaatg cgccgggcca aaccggggca gcgccgttgc
     5761 gacattcgat atcaaagaaa ccgcgtaccg cggcaccatc aagatgaaca tgggcggcaa
     5821 gaatatgatc atgtcggaaa cgcaaaaggg gaaaagaaca ggcgattgcc agtaattggg
     5881 tcggttatgg cgagccggca ttggctgccc cggacgcatg cttacggtcg ccatgagttt
     5941 taaccgtgcc ccgtttttgc ttgacagccg cttcggtctg gcttcagccg ggaacttccg
     6001 ccccgctttt ggaatccaga ggatatgtca gacttgcgcg catccggctt cagtttacga
     6061 cgggagatcg tggggagtct tttcgagtcc ctgtcggcga agacggcgaa tgggttagtc
     6121 aatgtgatca tcgacacgcc caaagggagt cgcaacaaat tcaagttcga cgagcaggtc
     6181 cagtgctttc gcttgagccg gattctgccg ctgggggcct gctttccgta tgattttggc
     6241 ggcatccccg gcacggcggc cgaagatggc gatccgctcg atgtgctggt cattgcccag
     6301 gagggcagct tcgtcggttg tcttcttacg gcgaaactca tcggtggtgt gcgggctgag
     6361 cagaccgaaa aaggaaggac catccgcaac gaccggctgc ttgccgtgcc ggtcaccaac
     6421 gccaatcccg ccgaattttc acacatcgac cagctaccat cggcgcgcgt agccgaaatc
     6481 gaacacttct tcgtgtccta caaccaggcg cacggccgtc aatttcggcc caccggacat
     6541 gccggtccgg acgaggcgga tcgggttctc gccgcggcaa tacagcgttt cgaagaatct
     6601 cgggaatgag ccaaggggcc ttcccgcgac agcgcggaat tcgtcgcggc g
//
LOCUS       DATMZQ010000108         7654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3884914, whole genome shotgun sequence.
ACCESSION   DATMZQ010000108 DATMZQ010000000
VERSION     DATMZQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3884914"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1507
                     /locus_tag="VN496_03350"
     CDS             137..1507
                     /locus_tag="VN496_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXF16011.1"
                     /translation="MNSKVAMTAFVLTLAVTLSYETNISVSAVPGARDPGVRTIRSDT
                     GAPIAGLSRTEQAFFLAGQDEFNEAETPDDGLGPTMNLDSCGGCHSQPATGGTSPAVN
                     PQVAFANQMGARNSIPSFIRRDGPVREVRFIRNGDGSADGGVHSLFTLYGRSDASGCF
                     LSQPDFEGEYGRGNAIFRIPTPVFGAGLIEQIPDRTILANQAATAPRRQQLGIGGKAN
                     IIVPFSTISGQSNHNGNDGTVARFGWKAQNKSLLIFSGEAYNVEMGISNELFPTEREE
                     ARGCQYAMTPNDVTNTAGTGGVDTMGATDKFALFMRFLAPPVPSTDTPGGASSISRGK
                     ALFGDTGCALCHTASMQTGNSSVAALRNKPVNLYSDLLVHDMGVGLRDGVSQGQAGPR
                     EFRTAPLWGLGQRLFFLHDGRSSDLVDAIKQHGSQGSEANGVIAIYNNLGEFQKQDLL
                     NFLRSL"
     gene            1674..1865
                     /locus_tag="VN496_03355"
     CDS             1674..1865
                     /locus_tag="VN496_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16012.1"
                     /translation="MNINHPNYRNAGIIDYRSIDKLERDADRFHWRLEKISGREMTMI
                     VVAYNRNGHPTARIVQSCL"
     gene            2352..2699
                     /locus_tag="VN496_03360"
     CDS             2352..2699
                     /locus_tag="VN496_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16013.1"
                     /translation="MRAQARAEPEQDVAQAPERQAQLVAELPVLPGEQAQAQVLLVPP
                     VERALVVPRAPVLAPALEVPRELRALLRERALVRAAQLAWVLARVRVPASQVEEARPV
                     PLEQEQLGRVARE"
     gene            2703..2978
                     /locus_tag="VN496_03365"
     CDS             2703..2978
                     /locus_tag="VN496_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16014.1"
                     /translation="MVQAPEEQLELRAQQPEQVPARVPPALQVGAAPALEQLAQRAPL
                     VLESPAVPAREAQREPRAPQPGRAREHQAQARLASQREQVQRVRAQQ"
     gene            complement(3676..3993)
                     /locus_tag="VN496_03370"
     CDS             complement(3676..3993)
                     /locus_tag="VN496_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16015.1"
                     /translation="MSKVEKLYDIVMNDAVEHVFIYALRHAMDTRALGVEFDAGRGPG
                     PHSEHTLTIGLRASPISVTGSDIPHDWLSAGTGYIDTRFSRSVTALLIELQKKAKQAG
                     LVI"
     gene            complement(4200..4727)
                     /locus_tag="VN496_03375"
     CDS             complement(4200..4727)
                     /locus_tag="VN496_03375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXF16016.1"
                     /translation="MGQHIKLYISQTSRHPMRFAAYDKAGKATIVVTKGMLSLLHDDE
                     PAWAGLLGHETAHLVKHHSEGRATAAAGAWAGGELVAIALSLLIPGFGGFVAGTAGGT
                     AANKALYGAYTRPQEAEADQLGLQWLVSAGYDPHGMQRLFELLAKQSSGPTFLSTHPG
                     AEDRAKMVRDYIDGK"
     gene            complement(4747..4950)
                     /locus_tag="VN496_03380"
     CDS             complement(4747..4950)
                     /locus_tag="VN496_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16017.1"
                     /translation="MEGFWAFLELAARKWAWECRHADGTTTRRGPFFSLAECLQDAKD
                     KGFMESKRDRRKIPRPPPAQGTK"
     gene            complement(4990..5325)
                     /locus_tag="VN496_03385"
     CDS             complement(4990..5325)
                     /locus_tag="VN496_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16018.1"
                     /translation="MKLFEALKKVRELERTQLPFIRSVIDFDILIEIGYAEETRRPMT
                     LKQVLLLNLSSRTTVRRRVLKLIKDGVVRRRADANDRRSSLLTISASNLKLMAKYGSA
                     MTAIWAAMA"
     gene            5328..5762
                     /locus_tag="VN496_03390"
     CDS             5328..5762
                     /locus_tag="VN496_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16019.1"
                     /translation="MNGIDLGPGMMVLLFSRWQGQLGTFLAPKCRRKASLISRQLYLK
                     WSFRIANSFCSRPLGSALSNKFALPGCRDILNGTSLAAELITGPRYTDGITARSARII
                     AIVFARFTDGLQKTLRCLVFQANNLLNHQRCLLAVKLLMEYS"
     gene            5802..6116
                     /locus_tag="VN496_03395"
     CDS             5802..6116
                     /locus_tag="VN496_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16020.1"
                     /translation="MRQLHKSFLRSFNAPTGAALALVAALTTGPLYALSDTDTPAAFR
                     FAAEANQGALTEKETRTVPGTAVAEGSLIWTRDGAGSPENLRRIAAIPKEEERLGSGC
                     SK"
     gene            complement(6205..7251)
                     /locus_tag="VN496_03400"
     CDS             complement(6205..7251)
                     /locus_tag="VN496_03400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXF16021.1"
                     /translation="MILFWIAHILGGLPLHAFINAALFTVFFIVLFYILFRSGLNKRF
                     RDASLTKPQILASALVILYVLAESPDNQGVVALIFLVAFLFGVFRLSTRELLGLSAVV
                     TTGYALVIARQSQIGMDPTELDRRLLNWIVLTAVLVFFSVMGGYISRLRKKAQESKDQ
                     LQRALMRIEHLATRDELTGVPNRRSLVDVLTIQQSRSERYGTTFSVLLLDIDFFKRVN
                     DVHGHRAGDLVLRSLAMAASSCMRTIDTFGRYGGEEFLIILDQTTLQSATTVAQRLCQ
                     CARDISFQDWAPELRISVSIGVTQYSRPEDWQVTIARADHALYRAKNGGRDRFEAELP
                     PVSDGSVAEEHLTA"
BASE COUNT         1675 a         2140 c         2198 g         1641 t
ORIGIN      
        1 ctgtccccgt atagctacag aaaagtagac atctgactac aagaaaatat ataggccttt
       61 gattttttgg aatacgcccg cacggcatac ggattgcgct tagaccggcg ccgtataaaa
      121 aatcaggagg acctgtatga acagcaaagt cgcaatgact gcttttgttt tgaccctggc
      181 cgtcacactc tcctatgaaa ccaatatttc tgtatccgcg gtcccgggcg cacgcgaccc
      241 gggcgttcgc accattcgct cggacacggg cgcgccaatc gcgggccttt ccagaactga
      301 acaagcattt tttctggctg gccaggatga attcaacgaa gcggaaactc ccgacgacgg
      361 acttggcccg accatgaacc tggacagctg cgggggttgc cattcccagc ccgctaccgg
      421 gggaacaagt cctgccgtca acccccaagt cgcgttcgcg aatcagatgg gcgcacgcaa
      481 tagcattccg tctttcattc gtcgggacgg gccggtgcgc gaggtgcgtt tcatacgcaa
      541 cggagacggt agcgccgatg gtggcgtaca ttcgctgttc accctttacg gtcgtagcga
      601 cgcatcgggc tgctttttaa gtcagccgga cttcgagggt gaatacggtc gcggcaacgc
      661 gatcttccgc ataccgacgc cggtgttcgg cgccggattg atcgaacaga ttcccgacag
      721 gacgattctg gccaatcagg cggcgaccgc accgcgcagg caacagctcg gcatcggcgg
      781 gaaggcaaac atcatcgttc cgttttcaac gatatcgggc cagagcaacc ataacggcaa
      841 cgacggaaca gtggctcgtt ttggatggaa agcgcagaac aagtcattgc tgatcttttc
      901 cggcgaggcc tataacgtag aaatgggaat cagcaacgag ttgtttccga ccgaacgtga
      961 ggaggctcgc ggttgccagt acgccatgac gccgaacgat gtaacgaata ccgctggcac
     1021 cggcggggtc gatactatgg gagcgacgga taaattcgcc ctgttcatgc gttttctcgc
     1081 accgcccgtg ccttcaaccg acacgccggg aggagcatct tccatttcgc gcggaaaagc
     1141 tctgtttggc gacaccggtt gcgcgttatg ccacactgca tcgatgcaga ccggcaactc
     1201 gtccgtcgcc gctttgcgaa acaagccggt gaacctgtat tccgatttgc tcgtgcatga
     1261 tatgggcgtc gggctgcgcg atggtgtgag ccagggacag gcggggccac gagaattccg
     1321 caccgctcca ctgtggggct tgggccagcg gcttttcttc ctgcacgacg gccgctcgtc
     1381 tgatctcgtc gacgcgatca agcaacatgg cagccagggc tcggaggcga atggcgttat
     1441 cgcgatctac aacaacctgg gcgaattcca gaaacaggat cttctgaatt tccttcgctc
     1501 gctatgaggg ccttgcgcaa aatagtcttg ccaaggattt cgccgtaacc cgaccggcac
     1561 aacctggatc tggcaaggtg taaaccgcca gtatcagttg tgccggtttc catttcatgc
     1621 gcgcctcttc gctgcgcttg cggaattact gtcgaattca aggggaacaa accgtgaaca
     1681 taaaccatcc gaattatcgt aacgccggaa tcattgacta ccgaagcatt gataagttgg
     1741 aacgcgacgc cgatcgcttc cactggcgcc tggaaaaaat atccggccgc gaaatgacaa
     1801 tgattgtggt tgcctacaat cgcaacgggc atccaaccgc ccgcattgtc cagtcctgtt
     1861 tataaggcgc gctatcgcat ttcagcaaga ctagacacca agtgcgaccc gtagcgatct
     1921 atcgcaattg tagaaatttt cgctgcacct cttaccctcg tagccctgct acgggcccgc
     1981 cttagatatt cacgcttccg gattcacttc ccgcctcgcg tgatggccga cgcgccatcg
     2041 ttttcgcgcc cgcgggactg atggttattt gtagaaaagt tacgagagtt ttggaatata
     2101 ccaagccgca ttaatcgtgc ggccacaccg gaacgctcca ttgcattggt ccgatgcttg
     2161 cagtagcgcc ccgctaagcg gcgagcagcg ttgaccgcgg aaaagttgca ccgtgaccgc
     2221 agagggaaat tgcgcgcacg cggcgcttca tcatttacaa atgcaaggag aggctttgat
     2281 gaacaggtta tttgggagtc ttatggttgg tgtaaccgcg ctggcacttg ttggcgttat
     2341 ggatactgca agtgcgcgcg caggcgcggg cggaaccgga gcaggatgtg gcacaggcgc
     2401 cggagcggca ggcgcaactg gtggcggaac tgccggtgct accgggggaa caggcgcagg
     2461 cgcaggtact gctggtgcca ccggtggaac gagcgctggt agtaccgcgg gcaccggtac
     2521 tggcgccagc actggaggtg ccgcgggaac ttcgggcgct tctgcgggaa cgggcgctgg
     2581 tacgggcggc gcagctggca tgggtactgg cacgagttcg agtgccggca tctcaggtag
     2641 aggaggcacg gccggtgcca ctggaacagg aacaactggg acgagtagca cgggagtaac
     2701 cggtggtaca agcaccggag gagcagctgg aacttcgggc acagcagccg gaacaggtac
     2761 cggcacgggt accaccggcg ctgcaggtcg gggcagcacc ggcactggaa cagctggcac
     2821 agcgggcacc tctagtactg gagtcgccgg cggtaccggc acgggaggcg cagcgggaac
     2881 ctcgggcacc gcagccggga cgggcgcggg aacaccaggc gcaggcacgt ctggcatcgc
     2941 agcgggaaca ggtccagcgg gtacgggccc agcagtagga ggagcaccgg gcgctagcac
     3001 cggcacggca ggcgcaactc cgggcggcgc cacaggactc ggtacaggca gcgtaggcgc
     3061 ctcggatgga ctcgccggag gtggcgcgcc tgcgaattcc gctgcatcgt ccagtggcgc
     3121 aaaagtcaac gccatgtcgc aagggaccag tgcggtccgc actcaggatt actccgcgga
     3181 agtgttgtcg tgcgaccgac tgaccgggat agacaaatcc gtctgcgtaa ccagtgcgaa
     3241 acgcaaagag actaacggta ccggaacctt ttaggctccc gcaagcatgc tgaaagctca
     3301 gacgacattg cagaaccgtg atcccgccat gtgccaaacg gactacgtgc gaacggttgc
     3361 ttgtccgatt caattgcagg agaattctta caccgcgtca gggagcatgt aagcgcatag
     3421 atcagtcagg atggccaacg gattgttaat agtcgactaa gtcggccaaa caatgtagtc
     3481 acctgtctgc tcgaagaaaa gagggtacac cgaaaggttg tacccctttt tttgtccgcc
     3541 tgttgccgca gccaggccgc taaagtgcgt gaaccgttgg acggttcaga acgtagcaag
     3601 gctttttagc gggatggcag ttgaaaccag cgtctcagct gctttgcctc ttcacggtcg
     3661 actgaacaga atctcctaga tgaccagacc ggcctgcttg gctttttttt gcagttcgat
     3721 caataacgcg gtcacggatc ttgagaatcg cgtatcgata tagccggttc cggcggaaag
     3781 ccagtcatgt ggaatatcgg aaccggttac cgatattgga gaagcgcgaa gcccgatcgt
     3841 aagcgtgtgt tctgaatgtg ggccagggcc gcgaccggca tcgaattcca ctcccagtgc
     3901 tcgcgtatcc atcgcgtgac gcagcgcata aatgaaaacg tgttccacgg cgtcgttcat
     3961 gacgatatcg taaagttttt caacttttga cacgctaggc gctcttgatg acccgccggt
     4021 gcgcggtctt accacagtca aaaaagaaca ttttttggtt cattgttcgg cggcgatagg
     4081 taccccgcat aatgtgacaa tgacagtatt accatgccat tctgatccgc gccagtagag
     4141 ggccgcgcag gtgttgtttt ccggtatttt caggcgcggt attgccgcct gcggaccgct
     4201 cacttgccgt cgatataatc ccgaaccatc tttgcacggt cttccgcccc ggggtgggta
     4261 gacagaaatg tcggcccgct ggattgcttt gcgagcaatt caaaaagcct ctgcatgccg
     4321 tgcgggtcat agcctgccga caccagccat tgcaaaccaa gttggtcggc ctccgcttct
     4381 tgaggcctgg tgtaagcacc gtacagcgcc ttgttcgcgg cagtgccgcc tgctgttccg
     4441 gcgacgaagc ctccgaatcc tggaatcagg agcgagagcg caatggcgac gagttcccca
     4501 ccagcccacg cgcctgccgc cgccgtggct cgaccttcgc tatggtgctt caccaagtgt
     4561 gccgtctcat gtcccagcag tcccgcccag gcaggttcgt catcgtgcag caggctgagc
     4621 attcccttcg ttacgacgat ggtggcctta ccggccttgt cgtaagcagc gaaacgcatt
     4681 gggtgccgcg atgtttgcga tatatataac ttgatgtgct ggccgatgcg ctaaaggacg
     4741 cgccggctat ttggttcctt gtgccggggg cgggcgcgga atctttcgtc tgtcgcgctt
     4801 cgattccatg aatcccttgt ccttcgcatc ctgcaggcat tcagcaagcg aaaaaaaggg
     4861 gcccctccgt gtagtagttc catcggcatg ccggcattcc catgcccatt tgcgcgcggc
     4921 cagttccagg aacgcccaga aaccttccat ggctaatgca caaccagaat tgcgttgacg
     4981 aaagacaact caggccattg cagcccagat ggctgtcatt gccgaaccgt acttcgccat
     5041 caacttgaga ttagaagccg aaatggtcaa gagagacgac cggcgatcgt tcgcatccgc
     5101 gcgccggcgt acgacaccgt cctttatcag tttgagcact cgccggcgca ccgtcgtccg
     5161 ggaactgaga ttcaacagga gtacttgctt gagtgtcatt gggcggcggg tctcttccgc
     5221 gtacccaatc tctatcaaga tatcgaagtc aatgacggat cttatgaatg gcaactgggt
     5281 ccgttccaat tctctgactt ttttcagcgc ttcgaatagc ttcatagttg aatggcattg
     5341 atcttggccc cggcatgatg gtgttgttat ttagtcgctg gcaaggacaa ttgggtacct
     5401 ttttggcacc taaatgccgg aggaaagctt cgctaatttc acggcagctc tacttgaaat
     5461 ggagctttcg aatcgccaac agtttctgca gtagaccgtt gggatcagcg ttgtcgaaca
     5521 aattcgcttt gcccggatgc cgcgatattc tgaacggcac ttcgcttgcg gcggaactaa
     5581 tcacggggcc ccgctatacc gatggcatta ctgctcggtc cgctcgcatt atcgcgatcg
     5641 tctttgcacg ctttaccgat ggtttgcaaa aaacacttcg atgccttgtg tttcaagcga
     5701 acaatttgct gaatcatcaa cgttgcttgc tggccgttaa attgctaatg gaatattcat
     5761 gagtcccgga tcgccgggaa tacctaatgt ttggaatcgc catgaggcaa ttgcataaat
     5821 cgtttttgcg ctcgttcaac gcgcccacag gcgctgcgct ggcgctggtt gcagcgttga
     5881 ccacaggacc gttgtatgcg ctaagtgata ccgacactcc agcagctttc cggtttgctg
     5941 cggaagcaaa tcagggcgcg cttacagaga aggagacgcg aacagtcccg ggaacagcag
     6001 tggctgaagg ctcactgatc tggacaagag acggggctgg ctctccggag aatttacgcc
     6061 ggattgcagc cattccaaag gaagaagaga gacttggttc tggatgttcg aagtgacagc
     6121 gcgggcccga ttctgatctt tcttttgatt ccggctcgta agctgacgtg accttggtgc
     6181 ccttgttcca cttggtcttg tacgtcacgc ggtcaagtgt tcctccgcga cgcttccgtc
     6241 cgatacaggc ggaagctccg cttcgaaacg atctcgcccg ccattttttg cccgatacaa
     6301 cgcgtggtca gctcgggcga tcgtcacttg ccaatcttca ggccggctgt actgcgtcac
     6361 gccgattgaa acgctgattc gcagttcggg cgcccagtcc tggaagctga tgtcacgcgc
     6421 acattgacac aggcgctgcg cgaccgtggt ggcgctttgc agtgtcgtct gatccaggat
     6481 aatcagaaat tcctctcccc cgtatcgtcc aaaggtatcg atagttcgca tgcaactcga
     6541 ggctgccatc gccaggcttc tcagcaccag atcgcccgcg cgatggccat gcacgtcatt
     6601 cacgcgcttg aagaagtcga tgtccagcaa aagaaccgaa aaggtcgtgc cgtagcgctc
     6661 ggagcgactt tgctgaatcg tcaagacatc gaccaggctt cgtcgattgg gaacaccagt
     6721 tagttcatcc cgggtagcga gatgttcaat acgcatcaat gccctttgca gttggtcttt
     6781 gctttcctgg gccttcttgc gtagccgact gatgtagcca cccatcaccg aaaaaaatac
     6841 aagaaccgcc gtaagcacaa tccagttgag cagtctgcga tccagctcgg taggatccat
     6901 gccaatctgc gactgccttg cgatgaccag tgcatagccc gtcgtgacaa cggcgcttag
     6961 ccccagcagc tcgcgcgtgc tgagccggaa aacaccaaac aggaaagcga ccaggaaaat
     7021 gagcgccaca acgccctggt tgtccggcga ttcggccagg acataaagaa taaccagtgc
     7081 tgaagcgagg atttgcggct tcgtcaggct tgcgtcccga aaacgcttgt tcagtccact
     7141 gcggaacaat atgtagaaga gcactatgaa aaaaacggtg aacagcgccg catttatgaa
     7201 ggcatgcaga ggaagcccgc cgagaatatg ggctatccaa aaaagaatca ctgccattgc
     7261 cgatgtggct gaagccatca gccagcgacg gatccgatgt ctttgcttcg gatccgttgt
     7321 caaaagaatg gggccaggcc gcattcgctt cactttcctt cggtgtcctg atcgttgggc
     7381 agtgtaatca ccgaagtata aatgcaatcc cttgccaaac cccaagcttg taaagcgttc
     7441 tatgtctgtt cagacgggtc agcggactga gaggcgcgcg gtgcctttgc gaaacggcat
     7501 ggcgacgtac ttgcgatagt tgtcgatgca ttggcgcctg cagctccgag atcgcagctt
     7561 gtgtcatgcc attgccagtt gtccgacggc gccatccatg tctagcggtg gtttatgcgc
     7621 gggaatgcca tcggtcgagg cgcctgcgat ttcc
//
LOCUS       DATMZQ010000109         6709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3193675, whole genome shotgun sequence.
ACCESSION   DATMZQ010000109 DATMZQ010000000
VERSION     DATMZQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193675"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VN496_03405"
     CDS             <1..234
                     /locus_tag="VN496_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HXF16022.1"
                     /translation="EGGYTVVGKLMPADTSITIGALSLGLAHGVKMLRPVTAGQPVRW
                     TDVAVDESSYAVQLRREMEQQFAPAAEPAIAAR"
     gene            346..1629
                     /locus_tag="VN496_03410"
     CDS             346..1629
                     /locus_tag="VN496_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF16023.1"
                     /translation="MISKPPRTRQIVAAVVGNALEWYDFVVYGFLTIIIARLFFPAQS
                     EYASLLLTMATFGVGFFMRPVGGIVLGMYADRKGRKAALQLIIGLMTLAMAMIAFAPP
                     YAAIGIAAPLIILLARLLQGFSTGGEFASATSFLVESAPPNRRGFYGSLQMVGQSLAA
                     LSGAVAGTLITRGLGPEQIDSWGWRVPFLFGLIIGPVGLYIRRYLEETDAFLDSRHTN
                     AVRNPLRTVFAQNGRGLAVTFGLVICGTISYYVVLVYMPTFAKTQLGMPLTDAFVAQV
                     IGLVCLTATIPLCGALSDRIGRRPILVAATIAYFVLLYPLFDWVYTAPSLLRLAIMQG
                     ILCTLVGVFFGPISTAIAEQFPTGVRSTGLAIAYNFAVMLFGGFAQFIVTWLIRETGS
                     PLAPAYYVMFGAVVGFVASVALVERYRDHQLPKAV"
     gene            1669..2127
                     /locus_tag="VN496_03415"
     CDS             1669..2127
                     /locus_tag="VN496_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16024.1"
                     /translation="MTTWNGRLGTMLMLAATFGGVTMKANTFAADTGEKTMTIVRIYT
                     GADNQSHFEDLKIPLKSTGKIGFISELMKATGVVFRETGGDYNYDFHTAPRRQYVVNL
                     EGEVEIEVGDGTKRILRSGDVLLAEDTTGQGHISRAVAGKSRKSLFITLD"
     gene            complement(2114..2986)
                     /locus_tag="VN496_03420"
     CDS             complement(2114..2986)
                     /locus_tag="VN496_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXF16025.1"
                     /translation="MNSPRSHLIDAIGRAHAVARADARIVCLVPSISELLFHLGAGDR
                     LVGRTGFCVHPKDNIRTVPKVGGTKSVDIEKIRALAPTHLIVNIDENEKSTVEQLATF
                     VPNVIVTHPLGPLDNPSLYRLLGGIFGREDKAEQLCATFRKAYADAITACAALPRERV
                     LYLIWKSPWMTVSRDTYISRTLAAIGWDTVQTDGKARYPTIELTPTVLANVDRILLSS
                     EPYAFRERHLEEIRQISAAATHYFVPTPMEEYDSPTDVSLINGEMTSWYGTRAIEGMK
                     YLSRLRTDKRTSRA"
     gene            complement(3012..3539)
                     /locus_tag="VN496_03425"
     CDS             complement(3012..3539)
                     /locus_tag="VN496_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXF16026.1"
                     /translation="MNIDKFDMAILQVLQNDARASLQAISEQVGLSSTPCWTRIKRME
                     SEGVIRGYTVRVDPPSIGLSETVIVQVTLESHTDETLNAFGKMLEQIPEVVEAYLVSG
                     DYDYYIRIAVKDTRDYERLLRERLYRIPGIRHSKSSFVLRTLKESVIPLSAAPVAISG
                     LPVSGGRKRRLARRK"
     gene            3718..5442
                     /gene="ilvB"
                     /locus_tag="VN496_03430"
     CDS             3718..5442
                     /gene="ilvB"
                     /locus_tag="VN496_03430"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HXF16027.1"
                     /translation="MSLLDTLVSLGVDTIFGYPGGAVLPVYDALHAEPRIRHILVRHE
                     QAAVHAAEGYARSTGKPGVVLVTSGPGVSNTITGLLDAMCDSIPVLCISGQVATSSIG
                     TDAFQECDALGLSRPVTKWNAQIRNANEVSALVRKALFVATHGRPGPVLLDFPKDVQL
                     ATYDRPTAAPEAFPELPRKTHRRGPLRQAVDLLKQAQRPVFYGGGGLINAGPGACAAF
                     TRLVQRTGAPCTLTLMGLGAYPASHAQFLGMLGMHGTLEANLAMHHADLVVCVGARFD
                     DRVTGKLSEFCPHAQMIHLDIDASSINKVVKATVPLVGDCGELLDALETELGAHTFEA
                     SRLEEWWEQIKQWRAVDSLRFDARSDSIVPQQLMRALQQAIDGCDAIVSTDVGQHQMW
                     AAQHLRFETPCRWLTSGGAGTMGYGLPAAIGAQVGNPDRLVVCMSGDASVLMNLQEMS
                     TAVQHRLPVKVLLCNNGYMGMVRQWQELNHGSRYSHSYTAALPDFVAVAKGFGWGARR
                     VSDPAELNAAIAECLASDGPFFLDVAVAPQENCFPMMAAGSGHHEIMLNKTTLYRAGV
                     TTVDQART"
     gene            5516..>6709
                     /locus_tag="VN496_03435"
     CDS             5516..>6709
                     /locus_tag="VN496_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXF16028.1"
                     /translation="MRQVSLEDKYLSDHDRVYLTGIQALVRLALVQRRRDATNGLNTA
                     GFISGYRGSPLGGLDEALWKAKKHLEAHHIKFQPGINEELAATAVWGSQQVEMLGESK
                     YDGVFAMWYGKGPGVDRCGDVFKHMNFAGTSKHGGVLLVAGDDHGAYSSSLPHQSDHL
                     FAASMIPVLYPCNVQEYVELGLHGWAMSRFSGCAVGFKALSDTVESSASIEADPFHVK
                     TLVPTDFAMPEEGLNYRLSNEPLGLQARKQEALLQDYKIYAAMAYARLNKINRTTIDS
                     PQARLGIIASGKSYMDVLEALEELGITEEKAAQIGIRVFKVSMPWPLEPDGVREFSKG
                     LDEILVVEEKRQVVEYQLKEHLYNWHPDVRPRVIGKFDDQGEWVHPRGEWLLPAKADF
                     SIAQIA"
BASE COUNT         1331 a         1918 c         2000 g         1460 t
ORIGIN      
        1 gaaggcggat ataccgtcgt cggtaaacta atgccggcgg acacgtctat aactattggc
       61 gccttgtccc ttggccttgc gcatggggtg aaaatgctgc ggccggtgac agccgggcaa
      121 ccggtgcgct ggaccgatgt cgcggtcgac gaatcaagct acgcagttca gcttcggcga
      181 gaaatggagc aacagtttgc gcccgctgcc gaaccggcca tagcggcgcg atagggtcga
      241 atgcgattgc actgaggttg gggggcgtgc gcggcttttc cagttccggg catagcgagc
      301 cagcctgacc ggtacaatgc gggtcccatc ccaacaggac gtcccgtgat atccaaaccg
      361 ccacggacca ggcaaatcgt cgcggccgtc gtcggcaatg ccctcgagtg gtacgacttc
      421 gtcgtctacg gcttcctgac catcatcatt gcgcgtttgt tctttccggc gcaaagcgaa
      481 tacgcgtcct tgctgttgac catggcgacg ttcggggtcg gatttttcat gcgcccggtt
      541 gggggcatcg tgctcggcat gtatgcggac cgcaaaggac gtaaggctgc gctgcaattg
      601 atcattggac taatgactct ggcgatggca atgatcgcgt tcgcgccgcc gtatgcagcc
      661 attggcatcg ccgcgccgtt aattatcctt ttggcgcgcc tgctgcaagg cttctcgact
      721 ggcggtgaat tcgcaagcgc cacgtcgttc ctcgtggaaa gtgcgccccc caaccgccgc
      781 gggttctacg gttccctgca aatggtcggg caaagtctcg cggcgctttc cggcgctgtt
      841 gcgggaacgc taataacgcg cggactcggt cctgagcaaa tcgactcgtg gggatggcgc
      901 gtaccgtttc tgttcggtct catcatcggt cccgtcggac tgtacatccg tcgctacctt
      961 gaagagaccg atgcatttct tgattcacgc cacactaacg ccgtacgcaa ccccctgcgg
     1021 acggtatttg cgcagaacgg gcgcggtctg gcagtcacgt ttgggctggt gatctgcggc
     1081 acaatctcct attacgtggt gctggtatac atgcccacct ttgcgaagac gcaactgggt
     1141 atgccattga ctgacgcatt cgtggcccag gtcatcggtc tcgtatgtct gaccgcaacc
     1201 attccgctgt gcggagcctt gtcagaccgc atcggtcgcc ggccgatcct ggtggccgcg
     1261 acgattgctt actttgtctt gctctatccg cttttcgatt gggtatacac ggctcccagc
     1321 ctgctcaggc tcgcgatcat gcaaggtatc ctgtgcacgc tcgtcggcgt atttttcggg
     1381 ccgatctcca ccgccatcgc ggagcagttt ccgaccggcg tacggtccac tggcctggcg
     1441 attgcctaca actttgcggt catgctcttc ggcggtttcg cgcaattcat cgtcacctgg
     1501 ctgatccgtg agactgggtc gccgctggcg ccagcgtatt acgtgatgtt cggcgctgtt
     1561 gttggctttg tcgcctcggt ggctctggtg gaacgctatc gcgaccacca actacccaag
     1621 gcggtgtgat catcagatgc tttattcatc tcataaggga agcgcgcaat gacaacatgg
     1681 aacgggcggc tgggtacgat gctgatgctc gctgcgactt ttggaggcgt tacgatgaaa
     1741 gcgaacacgt ttgctgccga taccggtgag aaaaccatga caatcgtgcg tatctatacc
     1801 ggcgccgaca accagtccca tttcgaggat ctcaagattc ccctcaagag caccggcaaa
     1861 atcggattca tctccgagct tatgaaagcg actggtgtcg tgttccgcga aaccggtggc
     1921 gactacaact atgattttca caccgcaccg cggcgccagt acgtggtcaa tctcgaagga
     1981 gaagtggaaa tcgaggtagg cgatggaacg aagcgcattt tgcgcagcgg cgatgtgctg
     2041 ctggccgaag acaccaccgg ccagggacac atcagccgcg cggtggctgg caaatcgagg
     2101 aaatccttgt tcatcacgct cgactagtac gcttgtcggt acgcaaccgg gacaggtatt
     2161 tcatgccttc gatcgcccgt gtgccgtacc aggaggtcat ttctccgttg atcagcgata
     2221 catctgttgg cgaatcgtat tcctccatcg gagtcggaac aaaatagtgg gtcgccgccg
     2281 cagagatttg acggatctcc tcgagatggc gttcgcggaa ggcatagggt tcggaagaaa
     2341 gcaggatgcg gtcgacattt gcaagcaccg ttggcgtgag ctcgatggtc ggataacgag
     2401 ccttgccatc ggtctgcacg gtatcccagc cgatcgcagc caacgtcctg gaaatatagg
     2461 tatcgcgcga tacagtcatc cagggtgatt tccagatcag gtaaagcacg cgttcgcgcg
     2521 gcaacgcggc acatgccgtg atggcatcag cgtaagcttt gcggaaggtc gcgcagagtt
     2581 gttctgcctt gtcctcgcga ccaaaaattc cgccgagcag ccggtacagc gatggattat
     2641 cgagcgggcc cagcggatgc gtcacgatca cgtttggcac aaatgtcgcg agctgctcga
     2701 cggtcgattt ttcgttttca tcgatattca cgatcagatg tgttggcgcc agagcgcgga
     2761 tcttctcaat gtcgacgctt ttggtaccgc cgaccttcgg caccgttcgg atgttgtctt
     2821 tgggatggac acaaaagccg gttcggccga ccaggcgatc ccccgcgccg agatggaata
     2881 acagttcact aatgctgggg acgaggcaga cgatgcgggc atcggcacgc gcgactgcgt
     2941 gtgctctgcc aatggcgtct atcaggtggg aacgcggact attcatttca gtcgccgact
     3001 ccaggcctgc cttacttgcg cctggctagc cttcttttcc tgccaccgga cacgggcaat
     3061 cccgatatcg cgaccggcgc agcagacaga ggaatcacgc tttccttcaa cgtgcgcaat
     3121 acgaagcttg atttgctgtg ccgtatgcct ggaatgcggt acagccgctc gcgcaacaac
     3181 cgttcgtagt cgcgcgtatc tttgacagcg atacggatgt agtaatcgta atctccggag
     3241 accagatagg cttccacgac ctcgggaatc tgctccagca ttttcccgaa ggcattcagc
     3301 gtctcatccg tatggctctc cagcgtgacc tggacaatca ccgtttcgga tagtccgatc
     3361 gagggcggat cgacccgaac cgtataaccg cggatcactc cttcgctttc cattcgcttg
     3421 atccgtgtcc agcacggcgt ggatgacagc ccaacctgct cgctgatggc ttgcagactg
     3481 gcccgggcgt cattttgcag cacctgcaat atcgccatgt cgaatttgtc gatgttcatt
     3541 ctatcgttga gcagttcatt agaaaacatt tccgatctat gtgcattatt gcaggatata
     3601 agatgctttt tctacgatgt cccgagtacg attgcactca tgaccaatct cgccctgcct
     3661 gacacgctgg tgccgcctgc aacgcaggcc gcacatcgcg ttgtcgaaac cggtgccatg
     3721 tcactgcttg ataccctggt ttcgctcggc gttgatacga tttttggtta tccgggtggg
     3781 gcagtactgc ccgtttacga tgcgctgcat gccgaaccac gcatccggca tatcctcgtc
     3841 cgccatgagc aggcggcagt tcatgcggcc gagggttacg cgcgttccac tggcaagccc
     3901 ggtgtggtac tcgtcacctc aggacccggc gtgagtaata cgatcacggg tctgctggat
     3961 gccatgtgcg attccattcc ggtgctctgc atcagcgggc aggtcgccac aagctcgatc
     4021 ggcacggatg cgttccagga atgcgatgca ctgggcttgt cgcggccagt cacaaaatgg
     4081 aatgcacaaa tccgcaacgc aaatgaagta tcggcgttag tgcgcaaggc gctgttcgtt
     4141 gcgacacacg gtcggccagg tcctgtgctg ctggactttc cgaaagacgt ccagcttgcc
     4201 acctatgatc ggcccaccgc ggctcctgaa gcattcccgg aattgccgcg caaaacccac
     4261 agacgtggcc cgctgcgcca ggcggtagat ctattgaagc aagctcagcg gccggtattc
     4321 tacggtggtg gcggattgat caacgcgggc ccgggcgcct gtgccgcgtt cacgcgcctc
     4381 gtgcaacgca ccggcgcacc ctgcactttg acgttgatgg gtctgggcgc ttatccggcc
     4441 tcgcatgctc agtttctcgg catgctcggg atgcacggca ccctggaagc caatttggcc
     4501 atgcatcacg ccgatctggt cgtgtgcgta ggcgcccgct tcgacgacag ggtgaccggc
     4561 aaattgagcg aattctgtcc gcatgctcaa atgattcatc tggacattga tgcctcatcc
     4621 atcaacaagg tcgtgaaagc cactgtgccg ctggtcggcg attgcggtga actgctcgat
     4681 gccctggaaa ccgaactggg tgcgcacacc ttcgaagcaa gtcggctgga ggaatggtgg
     4741 gagcagatca aacaatggcg ggcggttgat tcgctgcggt ttgacgcgcg ttccgacagc
     4801 atcgtccctc aacaactgat gcgtgctctg cagcaggcga ttgacggctg cgacgccatt
     4861 gtgtcgaccg acgtcggcca gcatcagatg tgggcggcgc agcatctgcg tttcgaaaca
     4921 ccctgccgct ggttgacctc gggtggcgcc ggcacgatgg gatacggtct cccagcggct
     4981 atcggcgcgc aggtaggcaa tcccgacagg ctggtagtgt gtatgagcgg cgacgcctcc
     5041 gtactaatga atttgcagga gatgtccacg gcggtccagc accggctgcc ggtgaaagtg
     5101 ttgttatgca ataacggcta catgggtatg gttcgacaat ggcaggaact aaaccatggc
     5161 agccgctaca gccatagcta caccgccgcg ctgccggatt ttgtagctgt ggccaagggg
     5221 ttcggctggg gcgcgcgccg tgtcagtgac cctgccgagc tcaatgcggc gatcgcggaa
     5281 tgtctggcca gcgacggccc cttcttcctg gatgtagcgg tcgcgccgca ggaaaactgt
     5341 tttccgatga tggcggccgg cagcggccac cacgaaatca tgctgaacaa gacgacgctg
     5401 taccgggccg gtgtgacgac ggtcgatcag gctcggactt gaattaggcg gcgagcggcc
     5461 ggtccaacgc ttgtcggcgc cttacggcgc aaataccgga ttacaggagt tggcaatgcg
     5521 gcaagtttcc ctggaagaca aatacctgtc ggatcacgac cgggtttacc tcactggcat
     5581 ccaggctctg gtgcgtttgg cgttggtcca gcgccggcgc gatgccacca atggtctcaa
     5641 cactgcgggc ttcatctcag gctatcgcgg ctcgccgctg ggcggccttg acgaggcttt
     5701 gtggaaagca aagaaacacc tcgaagcgca ccatatcaaa ttccagcccg gcatcaacga
     5761 agagttggca gccaccgctg tgtggggttc gcagcaggta gagatgctgg gtgaatcgaa
     5821 atacgacggc gtgtttgcta tgtggtatgg caaaggacca ggggtagacc gctgcggcga
     5881 cgtgttcaag cacatgaatt tcgccggcac ttcgaagcat ggcggcgtgc tgctggttgc
     5941 gggcgacgat cacggcgctt attcgtcttc cttgccgcat cagagcgatc atctgtttgc
     6001 cgcctccatg ataccggtgc tctatccctg taacgtgcag gagtacgtcg aacttgggct
     6061 gcatggctgg gccatgtcgc gcttttccgg gtgtgcagtc ggattcaagg cgctgtccga
     6121 taccgtggaa tcatcggcat ccatcgaagc cgatcccttc cacgtcaaga cgctggtgcc
     6181 gacggatttc gcgatgccgg aagaggggct gaactaccgg ctgtccaatg aaccacttgg
     6241 actgcaggcg cgcaagcagg aagcactgct gcaggattac aagatctacg cggcaatggc
     6301 gtacgcgcgc ctgaacaaga taaaccgcac cacgatagat tctccgcagg caaggctcgg
     6361 catcatcgct tcgggaaaat cctacatgga cgtgctggaa gcgcttgaag aactcggcat
     6421 caccgaggag aaggcagcgc aaatcggcat ccgcgtgttc aaggtttcca tgccttggcc
     6481 gctggaaccg gacggcgtgc gggaattctc caaaggcctg gatgaaattc tggttgtaga
     6541 agaaaagcgc caggtcgtcg agtatcagct caaagagcat ctttacaatt ggcatccaga
     6601 cgtgcgtccg cgcgtaatcg gcaaattcga cgatcagggc gaatgggtgc atccccgagg
     6661 cgaatggttg ttgcccgcca aggccgattt ttcgatcgcg cagatcgcg
//
LOCUS       DATMZQ010000110         5395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3194586, whole genome shotgun sequence.
ACCESSION   DATMZQ010000110 DATMZQ010000000
VERSION     DATMZQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3194586"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VN496_03440"
     CDS             complement(<1..457)
                     /locus_tag="VN496_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HXF16029.1"
                     /translation="MKPAWLQQRERGNMLGLRLAVVIAFFLGRNLARLLLYPVCLYFL
                     LFSVRSRRASRDYLSRVLERPPGFGDLFRHYLTFATVALDRFFLLNNRYELYDIEVHG
                     ADILQQTLDGGQGCLLLGAHLGSFEILRAAGTAHELEIAMVMYEDNATNI"
     gene            complement(595..1368)
                     /locus_tag="VN496_03445"
     CDS             complement(595..1368)
                     /locus_tag="VN496_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXF16030.1"
                     /translation="MPAHSKTHLVLMPSYNTGAKVYETTRNARQFWDPVWVVVDGSRD
                     GTAQGLQNMAALDAGLRLFVLPENRGKGAAILHALNIAVAEGYTHVLAMDADGQHQAD
                     QIPGFMRASMEHPDALILGKPVFDDTAPRIRVMGRQISNWCTNLETLWEGIGDSLFGL
                     RVYPAQPLLEVMESSRWMRRFDFDVEAAVRLVWRGVRPINLPAPVRYFRPEEGGVSHF
                     KYVRDNLLLSGMHLRLAAGFVMRLPVLLARKIRLWAMAN"
     gene            complement(1425..2234)
                     /locus_tag="VN496_03450"
     CDS             complement(1425..2234)
                     /locus_tag="VN496_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXF16031.1"
                     /translation="MALDTEPEVKQNRLAGIARTGYEYLVFYLGYLYFGVGCALTSLL
                     SGALHPLLPRRFGCRLGRRLTGLHFRAFLALLQASGLVKIDLAALDSLRGQHSIIIAP
                     NHPCLLDAVLIISRLPQIACIMKADIWDSFFLGGGARLSGYIRNDSPVSLVRRSTQEL
                     RAGHQLLVFPEGTRTREPYINHFRGGFALMAKKSRAEIQTIFIETQSDFLNKGWPPLK
                     KPAFPLIYRARLGKRTTVEGDVRTFVTNLEQYYRHELQASAMPSFRRTGSR"
     gene            complement(2294..3160)
                     /locus_tag="VN496_03455"
     CDS             complement(2294..3160)
                     /locus_tag="VN496_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HXF16032.1"
                     /translation="MRAKSAAARFPALSLQAAVTVIAVGAGTIPGAYSAERPLWEAGV
                     GVTVLDFPDYRGSDERHTLVLPIPYLVYRGEFLKSDRESVRGEFYHGDRLELDLSVNG
                     SIPVDSRENSARQGMSNLDPTLQIGPSLSVSLMRSEDMHLKLRFPLRAVLATDLKHVT
                     DQGWVFEPQINLDVYNRFPGPGWDLGFAAGPLYGNKRYHNYFYGVSPQDVTAERPAFE
                     ASGGYGGFQFIATLSKRFHSYWVGGFVRADTLSGATFENSPLVRQKESFAGGIAVSWV
                     FGQSQDMVEADE"
     gene            complement(3160..4497)
                     /locus_tag="VN496_03460"
     CDS             complement(3160..4497)
                     /locus_tag="VN496_03460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXF16033.1"
                     /translation="MPANSSPMGTSEAVAPCDVFVLGGGPGGSTIAALLAERGWNVVI
                     AEKDRHPRFHIGESLLPLNMPLFEHLGIRDEVERIGMPKFGAEFNSPKHPDPVTFDFS
                     DALDATYPSSFEVRRSQFDEILFRTAVRKGAHAFEACKVTNVQFLPKENIHIEASTSE
                     GTQAWRARFFVDASGRDTFLANRFGIKKRNRLHNSAAMYGHFTGAQRLPGKAAGNISM
                     FWFEHGWFWFIPLLDGTTSVGAVCWPYYMKSRKTSPTSFFMDTVAMVPKLAQRLRDAK
                     LTHEVTATGNYSYSASRMYGDRYILVGDAFTFVDPVFSSGVMLAMQSAYAGAEAVDAC
                     LRDPARAHAKLRQFDRAVRSGVKSFTWFIYRMTNPTIRQLFMAPSNALGMQAAVLSLL
                     AGDLFRGTPIHWRLRAFKALYYCLNLAHPLRSFAGWRNRRRLLRERLAGMPQS"
     gene            4725..>5395
                     /gene="fabG"
                     /locus_tag="VN496_03465"
     CDS             4725..>5395
                     /gene="fabG"
                     /locus_tag="VN496_03465"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXF16034.1"
                     /translation="MKTALVTGGSGAIGASICRALAAQGLKVLVHANHRLEEARQLAV
                     QLHAGGAEAKAVCFDVTDRDATARVLAELTARGPIQVLVNNAGVHDDAPLAGMSGAQW
                     DHVIDVSLNGFYNVTHPLLLPMIGTRWGRIINVSSVAGVIGNRGQVNYAAAKAGLHGA
                     TMSLALELASRGITVNAIAPGVIASPATQQAFPKDSIEALVPMKRAGTTEEVAALVAF
                     LASEQA"
BASE COUNT         1154 a         1593 c         1618 g         1030 t
ORIGIN      
        1 taatgtttgt tgcattgtcc tcgtacatga ccatcgcgat ttcgagttca tgcgcagtgc
       61 cggcggcgcg caggatttca aagctgccca ggtgtgcgcc cagcagcagg catccctgtc
      121 cgccgtccag cgtctgctgc aagatgtccg cgccatggac ctcgatgtca tataactcat
      181 agcgattgtt gagcaagaaa aagcgatcga gtgcgaccgt ggcgaacgtc agataatggc
      241 ggaaaaggtc gccgaagcca ggcgggcgtt ctagcacgcg agacaggtag tcgcgggagg
      301 cgcgccggga tctgaccgag aacaataaaa aatagagaca caccggatag agcaggaggc
      361 gcgccaggtt tctgccgaga aagaatgcaa tgacgacagc cagtcgcaaa cccaacatat
      421 tgccgcgctc gcgctgctga agccacgctg gtttcactgt cggaactcca aacttggcaa
      481 aagccagggc acagggcggc gcccggtatc gcgcaaaggc gcgggcaatg cgaatggctg
      541 gcaggtcatg ccgtcatggt aatggatctg gtgctcgggg cgctttgcct gcgcttaatt
      601 cgccatggcc cacaggcgga tttttctggc cagcagaaca ggcaggcgca tcacgaatcc
      661 ggctgcaagg cgcaggtgca ttcctgaaag cagcaaatta tcgcgcacgt acttgaaatg
      721 ggacacccca ccctcttcag ggcgaaagta acgcaccggt gcaggcaggt tgatgggccg
      781 cactccgcgc cataccagcc gcaccgcagc ttccacatcg aaatcgaagc gccgcatcca
      841 gcgacttgat tccataactt ccaacaaagg ctgtgcgggg tacacgcgca gtccgaacag
      901 ggagtcgccg attccttccc acagcgtttc caaattcgta caccagttgg agatctgcct
      961 gcccatgacc cggatgcgcg gggcggtgtc atcgaatacc ggtttaccca agataagcgc
     1021 gtccggatgt tccatggagg ctctcatgaa gccgggaatc tggtctgcct ggtgctggcc
     1081 gtcggcatcc atggccaata catgcgtgta accctccgcg acggcgatgt tcaacgcatg
     1141 caggatcgca gcacctttgc cgcgattctc cggcagcacg aacaatctca ggcccgcatc
     1201 caatgctgcc atgttctgca gtccctgtgc ggtcccgtcc ctgctgccgt ccactacgac
     1261 ccacacggga tcccagaatt ggcgggcgtt gcgcgtggtt tcataaacct tggcgccagt
     1321 attgtagctg ggcatcagca ccagatgagt cttggagtgg gcaggcatgg caatgtggac
     1381 tatgtcttca gcatgcagtt ccgtttaccc gcaaggggcc ggcatcaccg gctgcctgtt
     1441 ctgcggaagg aaggcatagc ggacgcctgt agttcatggc gatagtactg ttccagattt
     1501 gtcacaaatg tcctgacatc gccctcaacc gtagtccgtt tgccaaggcg tgcccggtaa
     1561 atgagcggga acgccggctt tttcagcggc ggccaaccct tgttcaggaa gtcgctttgg
     1621 gtctcaatga atatggtttg gatttcggca cgggattttt tcgccatgag cgcgaagccg
     1681 cccctgaaat ggtttatgta aggctcgcgc gtgcgggtac cctccgggaa caccagcagc
     1741 tgatggccag cgcgcagttc ctgcgtcgat cgccgcacca ggctcacggg cgagtcgttt
     1801 ctgatgtagc cggacagtct tgcacccccg cccagaaaaa agctgtccca gatatctgct
     1861 ttcatgatgc aggcgatctg cgggagccgc gagataatca gcacggcatc cagcaggcag
     1921 ggatggtttg gggcaatgat aatggaatgt tggccgcgca acgaatcgag cgcagcaagg
     1981 tcgattttaa ccaggccgct tgcctggagc agcgccagga atgcgcgaaa atgcagtccg
     2041 gtcaagcgcc gtccaagccg gcaaccaaac cttcggggca gcaacgggtg caaggcgcca
     2101 ctgagcagcg aggtcaatgc gcatccgacc ccgaagtata ggtagccaag atagaaaacg
     2161 aggtattcat agcccgtccg cgcaatacca gccagccggt tctgcttgac ctcgggttcc
     2221 gtgtccaggg ccatcagggc agagtcgctc cggacggccc ccagcgatgg cttttttgtg
     2281 ttggctggcg gggtcattcg tcggcctcaa ccatgtcctg cgattgccca aatacccacg
     2341 aaaccgcaat ccctcccgca aatgattctt tttggcgcac gagcggacta ttttcgaagg
     2401 tggctccgga tagcgtgtcg gcccgcacga aaccgccgac ccaataagaa tgaaaacgct
     2461 tgcttagcgt cgcgatgaac tgaaagccgc cataaccgcc gctcgcctcg aaggcgggcc
     2521 gctcggcagt cacgtcctgc ggggaaacgc cgtagaaata attgtgataa cgcttgttgc
     2581 cgtacaaagg accggcagca aaacccaggt cccagcccgg accgggaaag cggttgtaga
     2641 cgtcaaggtt gatttgcggc tcgaacaccc agccctggtc cgtcacatgc ttcaagtccg
     2701 tggccaaaac ggcgcgcagc ggaaaacgca gcttcaggtg catgtcctcg gaccgcatca
     2761 ggctgaccga cagactgggc ccgatctgaa gcgttggatc gaggttcgac ataccctgac
     2821 gagcggagtt ttcacggctg tccaccggaa tggatccatt cacgctcaaa tccagttcca
     2881 gccggtcgcc atggtagaac tcgccacgaa cgctttcacg gtcgcttttt aaaaattcgc
     2941 cgcgatagac cagataagga atgggcagta ccagcgtgtg ccgctcgtcc gacccgcggt
     3001 aatccgggaa gtccaggacc gtgaccccga ctcccgcctc ccatagcggc ctctctgcgc
     3061 tgtatgcccc cggaatggtt ccagcgccaa cggcaatcac ggttacggca gcctgcaacg
     3121 acagcgccgg aaagcgagcg gcggcggact tcgctcgcat caggattgcg gcattcccgc
     3181 cagtcgttcg cgcaacaggc gccggcggtt tcgccagccc gcgaagctgc gcagcggatg
     3241 cgccagattc agacaataat agagagcctt gaaggctcgc agccgccagt ggatcggcgt
     3301 accgcgaaaa agatcccccg ccagcaggga cagcacggcc gcctgcatgc ctaatgcatt
     3361 gcttggtgcc atgaacagtt gccgaatggt cggattcgtc atccgataga tgaaccaagt
     3421 gaagcttttc acaccgctgc ggaccgcgcg atcgaattgc cgcagcttgg catgcgcgcg
     3481 cgccggatcc cgcagacatg catccacggc ttccgccccg gcataggcgc tctgcatggc
     3541 cagcatgacg ccggaggaaa ataccgggtc aacaaaggtg aaggcgtccc caaccaggat
     3601 gtagcgatca ccgtacatgc gcgaagcact gtatgagtaa ttgcccgtcg cggtgacttc
     3661 atgcgtgagc ttggcgtcgc gcaggcgctg cgccaatttg gggaccatcg ccacggtatc
     3721 catgaaaaag ctggtcggac tggtcttcct ggacttcata taatacggcc agcatactgc
     3781 gccaacgctg gtggtaccgt ccagcagagg gatgaaccaa aaccagccat gttcgaacca
     3841 gaacatgctg atgttgcccg cggccttgcc cggcaggcgt tgggcaccag tgaaatgtcc
     3901 gtacatcgcc gcgctgttgt gcaaccggtt gcgctttttg attccgaacc ggttggccag
     3961 aaaagtatcg cgtcccgacg catccacaaa aaaccgggca cgccaggcct gcgtaccttc
     4021 gctggtgctc gcctcgatat gaatattttc cttcggcagg aactgcacat tggtgacctt
     4081 gcatgcttca aaagcatgcg cgcctttgcg cacggcagtg cggaacagga tctcgtcgaa
     4141 ctgggagcgc ctgacctcaa aggaagacgg gtaagtcgcg tccagcgcgt cgctgaaatc
     4201 gaatgtgacc ggatcgggat gtttggggga attgaattcg gcgccgaact tgggcatgcc
     4261 gatgcgttct acttcgtctc gaatgccgag atgttcgaac agcggcatgt tcagcggcaa
     4321 cagcgattcg ccgatgtgga agcgcggatg tcgatctttc tccgcgatga ctacgttcca
     4381 gccccgttcg gcaagcagcg cggcgatggt cgagccgccc ggcccgccgc cgagcacgaa
     4441 gacatcgcat ggggcaaccg cttccgatgt gcccatgggg cttgagtttg ctggcatgaa
     4501 cggattctac tatggcggat aagtttggct gccgttcgcc tgcgcagttc gccggccgcc
     4561 cgatcctggc aagccgcttg cggtggaagc tagtccggtt gcgctgacac ttccgttggg
     4621 cacggcatcg attgcatgcg atcatccgtt tgccggtgct gcgacgccag acgaacgtac
     4681 gatagccgca aatgccggga accatcagaa tataggggga ggcattgaaa accgcgttgg
     4741 tcaccggcgg cagcggggca atcggagcat cgatttgccg cgcgctggct gcacaagggc
     4801 tgaaagtgct tgtgcacgca aaccacaggc tggaagaggc gcggcaactt gcagtccagt
     4861 tgcatgcagg cggtgcagaa gcgaaggcgg tttgtttcga cgtaaccgat cgcgacgcaa
     4921 ccgcccgggt gcttgccgaa ctgaccgcgc ggggtcccat tcaggtcctc gtcaacaacg
     4981 ccggcgttca cgacgacgcg cctctggctg gaatgagtgg cgcgcaatgg gatcacgtga
     5041 tcgacgtatc gctgaatggt ttttacaacg tcacccatcc gctcctgctg ccgatgatcg
     5101 gtacacgttg ggggcgaatt atcaatgttt catcggtggc cggcgtgatc ggtaatcgag
     5161 gccaggtcaa ctatgcggcg gcaaaggcag gcctgcatgg cgcgacgatg tcgctcgcgc
     5221 tggaactggc gtcgcgcggc atcacggtga atgcgattgc acccggcgta atcgcgtcgc
     5281 cggcaacgca gcaggctttt cccaaggaca gcatcgaagc gctggtgccg atgaaacgcg
     5341 ctggcaccac cgaagaagtg gcggcacttg ttgctttcct tgcctcggaa caagc
//
LOCUS       DATMZQ010000111        22336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3497828, whole genome shotgun sequence.
ACCESSION   DATMZQ010000111 DATMZQ010000000
VERSION     DATMZQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3497828"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /gene="alr"
                     /locus_tag="VN496_03470"
     CDS             complement(<1..787)
                     /gene="alr"
                     /locus_tag="VN496_03470"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXF16035.1"
                     /translation="MARPIQATFDLAALKHNLAVARRLAAGASTMAVIKANAYGHGLL
                     RVAKALGDADGFALLELDDAVRLREAGYRQRILLLEGCFTEEELPVAVHHKLTIMVHA
                     REQLHMLQAMPAKDKLDVFLKFNTGMNRLGFPVTKAGEVIEQTKSLPCISRIVLTTHF
                     ADAEGPGGIQTQMREFNSCVARPDYERSLANSAALLRFPEAHGNWVRPGLMLYGASPL
                     HDISAEKLGLQPVMTLKSEVIAIQKLKPGDTVGYGGVFTADHDM"
     gene            989..2221
                     /gene="lplT"
                     /locus_tag="VN496_03475"
     CDS             989..2221
                     /gene="lplT"
                     /locus_tag="VN496_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HXF16036.1"
                     /translation="MKRGFYTILAAQFFSALADNALLFAAIALLASLHAQAWQTPVLQ
                     QFFVFAYIVLAPFVGPFADSLPKGRVMFVANAVKLLGCVAMLMGTHPLLAYGLVGVGA
                     AMYSPAKYGILTEYLPHQKLVLANGWMEGLTVAAIILGSILGGIMVSARFAGYIFSRI
                     DFPLVETGIDTPPELAITVILMLYFSAAVINLYIPKLAIDHKLQKRNPVFIMQDFWHS
                     FRLLWRDPLGQVSLAVTTLFWGAGATLRLIVLTWASMALKFSLEKATQLTAVVAFGIA
                     LGSVLAAKYVSLKRAVRVLPVGIAMGVLVMTMVFVNDWRIAIVLLVLIGAMGGYFVVP
                     MNALLQHRGHLLMGAGHSIAVQNFNENLSILVLLGVYALMIKVDVSIRGIIITFGLFI
                     AVAMGYLTRKHWHDQDSK"
     gene            complement(2236..2733)
                     /locus_tag="VN496_03480"
     CDS             complement(2236..2733)
                     /locus_tag="VN496_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXF16037.1"
                     /translation="MNMSRLLRHWMSPAWIVRRSFSPAALNRIQGAIAESETKHGGQI
                     RFAVEHALDTAALVRGISAAQRALQVFADLGVWDTEHNNGVLIYLLLADRDVEIIADR
                     GIHARVGTQGWETVCREMEAAFRQGHFEQGVLTGIERVSALLESQFPDPRPGHNELSD
                     RPVML"
     gene            complement(2735..3601)
                     /locus_tag="VN496_03485"
     CDS             complement(2735..3601)
                     /locus_tag="VN496_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXF16038.1"
                     /translation="MSMLVRAAIFAVPFLFSLICLADDRPIPALTGRVVDETGTLTAE
                     QKRALESKLQDFEQHKGSQIVVLITATTFPEPIESYSIRVADAWKIGRKGISDGIVIV
                     VAKSDRVMRLEVGYGLEGVIPDAVARRLIDEVFIPGFREGNFYEGINAGIDRLIKVID
                     GEPLPDVKVSDRGSGDLRSLESYFVLFLVATLAFGGLLRHLLGRLPAALIVGGGIGFL
                     AWLIVAPLIIAIVVGAVAFLVTLSAGASIPGRYGGGGFGGGGFGGGGFGGGGGFSGGG
                     GGFGGGGASGRW"
     gene            complement(3607..4215)
                     /locus_tag="VN496_03490"
     CDS             complement(3607..4215)
                     /locus_tag="VN496_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXF16039.1"
                     /translation="MNRIVVLWLLVAVTFGLNGCGYNTLQSQDEGIRAAWGEVLNQYQ
                     RRADLVPNLVNTVKGFAAQEKDVLLGVTNARAKVGGIQATPELVNDPAAFSKFVAAQG
                     EMTSALSRLLVVAENYPQLKSDANFRDLQAQLEGTENRITVARQRYIRAVQEYNITVR
                     SFPSNLTAMVFGYKPKPSFTVENEQAISKPPTVDFSKPEPKK"
     gene            complement(4248..4793)
                     /locus_tag="VN496_03495"
                     /pseudo
     CDS             complement(4248..4793)
                     /locus_tag="VN496_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(4996..5457)
                     /gene="rimI"
                     /locus_tag="VN496_03500"
     CDS             complement(4996..5457)
                     /gene="rimI"
                     /locus_tag="VN496_03500"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXF16040.1"
                     /translation="MSAQLDNLPEFRPMLATDLDRVMDLEPALYSHPWTRGNFSDSLT
                     AGYSCWIVECNRTLAGYGVLMIGVREAHLLNLSVATAWQRRGYGRMLLQHFINIARAS
                     DAAQLFLEVRPSNSAARQLYIGFGFRDITVRRGYYPAGRGREDAILMGISL"
     gene            complement(5454..6335)
                     /gene="tsaB"
                     /locus_tag="VN496_03505"
     CDS             complement(5454..6335)
                     /gene="tsaB"
                     /locus_tag="VN496_03505"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXF16041.1"
                     /translation="MKVLGMCTLLFCVMTGEAGATLIIVAVPAVVDRPRCSSNYNQLN
                     AAFSLQLKSVVTQASLRPETSGPALNLLALDTSTEYCSVALMRDGELTYLETHAVQRH
                     SELILPMIEQLLRAAGMAVGQLGGIAFGAGPGSFTGIRIACGVAQGLAFGAELPVVPV
                     GTLAALAQEAGGTRVLACLDARMGEIYHASYRRDDDKGWTEMTCPAVGPASSAPVLEG
                     DGWLGCGNGFAVYGDALAKRYGGQLVRVEAKLHPHARSIARLAMPVLAAGGGVPAEQA
                     LPLYVRDKVALKMHEQR"
     gene            6616..7392
                     /locus_tag="VN496_03510"
     CDS             6616..7392
                     /locus_tag="VN496_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16042.1"
                     /translation="MELSVKGRRVLITAGAGGIGRAMTDVFHAAGARVHICDVVQASI
                     AETQRAFPGVSGTLADVSRLKDVDQLFGEVRQHLGGLDCLINNAGIAGPTGKVEDIPV
                     EEWERCINVDMNGMFYCTRLAMPLLKAAGGGSIINLSSAAGRLAFPFRTPYSAAKWGV
                     VGYTKSLSMEVGPDNIRVNAIQPGVVEGERINRVIEAKAKALGISFEEQKKISLERVS
                     MHRMVSPYDIANMALYLASDAGRNITGQAISVCAGVEMLG"
     gene            7416..8357
                     /locus_tag="VN496_03515"
     CDS             7416..8357
                     /locus_tag="VN496_03515"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXF16043.1"
                     /translation="MEKVAIIGCGLIGRAWAMVFARAGHSVKLYDAAPGSAQQALLLI
                     RQGLEQLHQFGLIAESADIVLSRMSTVTTLADAVSDADYVQENTAEKLDVKREVYRQM
                     DAAAPAHCILASSTSTIQTSRFSESIKGRHRCIVAHPVNPPHIVPVVEISPAPWTSAE
                     VVAKTRALHEKVGQVAITVKKEVEGFILNRLQAALLLEAWRLVKEDYVSVEDLDKCIK
                     DGLGLRWSFMGPFETIDLNAPGGVPDYAKRYGPVLHGMMSNTKNEQWDEALIAKVDGE
                     RRKIMRQDRHAEREAWRDRRLMALIAHKREAAKKIGN"
     gene            8380..9306
                     /locus_tag="VN496_03520"
     CDS             8380..9306
                     /locus_tag="VN496_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXF16044.1"
                     /translation="MANKVIITCAVTGAIHTPSMSPHLPITPDEIAQAAIGAAKAGAA
                     VVHLHAREPKDGRPTQDPKMFLQFLPKIKAASDVVINLTTGGAPTMSIEDRLQPALQL
                     KPEVASLNMGSMNFGLYEMIPRVTQWKYEWEKPYLAGSDDRIFRNTFKDIAHILQSCS
                     NNDTRFEIECYDIGHLYTAAHFLDRGLVKPPLFIQSVFGIRGGIGPHPEDVLHMRRTA
                     DRLFGDQYQWSVLGGGRNQMYIATQSAVMGGNVRVGLEDSLWIGKGQLAKANADQVSK
                     IRRILEELGLEVATPEDARNILKLKGKNNVNF"
     gene            9432..11195
                     /locus_tag="VN496_03525"
     CDS             9432..11195
                     /locus_tag="VN496_03525"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXF16045.1"
                     /translation="MADQVMRQRLAALLAADVAGYSRLMATDERATVEALDAGRAIFR
                     RLIEANQGRVIDMAGDSVLSIFETAGGAVSAALSIQKELSSASCSLPEDRKMRFRIGV
                     HLGDVIEKADGTIYGDGVNIAARLEALADPGGITVSDAVQVAQRNRQPVVFEDLGEHD
                     VKNIAHPVRAFRVRSIGDAPEAGPTSPRFALPDKPSIAVLPFDNMSGDPEQEFFADGV
                     VEAITATLSRIRSFFVIARNSAFVYKGKAIDVQHVGRELGVRYVLEGSVQKSGTRVRI
                     TVQLIDTSTGAHVWADRVDGTLEDTFDLQDRITERVAGALQPSIRLAEIERARRKRPQ
                     ELGAYDYTMRAMPYVWVLEKDASAQALILLGEALAIDPEYPMALSLAGWCHAQRLVYN
                     WTEDIDVTRREALRGAETAAQHGGDDPLVLAVLGAVHTILRNMGTARIMLERAVALDP
                     NAAWAWSRLGWIENYSDRPGHAIEHFERALRLSPLDPMNFNNYVGIASAHEIAERYDE
                     AVAQYRRGLQERPHARWILRNLVSSLAGAGRIEEAREEYLQLCEEYPGLTAAKFRNAM
                     VFSPAVLDRMIANLKKVGLPD"
     gene            11213..12334
                     /gene="gcvT"
                     /locus_tag="VN496_03530"
     CDS             11213..12334
                     /gene="gcvT"
                     /locus_tag="VN496_03530"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXF16046.1"
                     /translation="MASETALLDTPLRGLHVELGAKMVAFAGYAMPVSYSTGIIAEHR
                     HCRAAAALFDVSHMGQLRVTGADAAGALESLMPADIVDLGVNRQRYGFFTNASGGILD
                     DLMVTRREDDIFIVVNASCKSADIAHLTACIGQRCSVEALPQLALLALQGPQAAVALS
                     RLNAAVGKLAFMTGMRARLNSSDCFVTRSGYTGEDGFEISVEAPAAQTLARVLLAQPE
                     VKPAGLGARDTLRLEAGLCLYGHDIDQTITPVEAGLTWAVAKVRRAGGVRAGGYPGAT
                     VLENQIARGCAVKRVGLTGSQRIPVREGAIITDLQGGKLGKVTSGTLSPSLNYPIAMG
                     YVATDKANVGQNLFSEVRGKIQPMGVTTMPFVPHRYHRV"
     gene            12412..12786
                     /gene="gcvH"
                     /locus_tag="VN496_03535"
     CDS             12412..12786
                     /gene="gcvH"
                     /locus_tag="VN496_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXF16047.1"
                     /translation="MTTRYTAEHEWINRDDDAAATVGITQHAQDALGDIVFVDLPTVG
                     TAYKKGDVAGVIESVKAAADLYMPVSGEVVEVNEALRNDPSLANSDPHGAGWFFKMRV
                     NDKVELEQLLDAAAYDALVKSV"
     gene            12820..15741
                     /gene="gcvP"
                     /locus_tag="VN496_03540"
     CDS             12820..15741
                     /gene="gcvP"
                     /locus_tag="VN496_03540"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HXF16048.1"
                     /translation="MSFYQPLDPLDTHSEFVARHVGIDQEDERHMLETIGVPSRTELI
                     KAIVPGSIARKKPMRLPEPVGEAQALAELKTIAGHNSVLKSYIGQGYHGTYTPGVILR
                     NVLENPAWYTAYTPYQAEISQGRLEAMMIFQTMVCDLTGLPIAGASLLDEATAAAEAM
                     ALALRMTTTRSKTFFVADDVLPQTLEVLHTRAKPLGVTIVTGAARDAGRAEAFAVLLQ
                     YPGVTGEVHDLKPLVDSAHQREALVIVAADLLAMTLLVSPGDVGADIAVGNTQRFGMP
                     MGNGGPHAAYLATREAFKRSLPGRLVGVSVDMHGAPAYRLALQTREQHIRREKATSNI
                     CTAQVLPAVVASMYAVYHGPDGLRRIAKRVASYAAILAAGARHLGCDIVHSTGFDTVQ
                     CTTGARTEAVLQAARECGYNLRRSSPTTIGITLDETTTSEDILALWPVLAHAGNATRL
                     AQVSKLSELWAELRKDVAPLFPASFARASSFLTHPVFNRHHNETDMLRYLRSLADKDL
                     ALDRSMIPLGSCTMKLNATSEMIPMTWPEFANVHPFAPPEQLAGYAQLREQLEGWLGQ
                     TTGYDGVSLQPNAGSQGEFAGLLIIKAFQESRGQTQRNVCLIPESAHGTNPASAQMAG
                     LRVVVTRCDARGNVDLADLRAKCEQFSDTLAAVMITYPSTYGVFDPQITEICALVHQY
                     GGRVYVDGANMNALVGLAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVVSDLIP
                     FLPAHRTAGMAGNSPVGAVSAAPLGNASVLPISWMYIRMMGAQGLQTASEIAILSANY
                     VAARLAPYYSVHFSGNIADIKGGGVAHECILDLRPLKDSSGVTAEDVAKRLIDFGFHA
                     PTLSFPVAGTLMVEPTESEPLRELDRFCEAMISIRAEIGRVESGEWPQDDNPLKNAPH
                     TAEALLKAEWLHPYTREQAAFPDTALRKSKYWVPVARVDNVWGDRNLFCTCPPLDDYQ
                     Q"
     gene            15876..16937
                     /locus_tag="VN496_03545"
     CDS             15876..16937
                     /locus_tag="VN496_03545"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HXF16049.1"
                     /translation="MTQNHKIAVIAGDGIGKEVMPEGVRVLEAAARKFGIALTFDHFD
                     WSCDNYDRHGWWMPPDWRSRIERHDCIFFGATGWPAKVPDHKSLWDSLILFRRGFDQY
                     VNLRPVRLMPGITSPLAGRKVGDLDYYVVRENTEGEYSAVGGRLFEGTEREMAMQQSI
                     FTRKGVDRIMRFAFELSMKRARKLLTSATKSNGISITMPYWDERFAAMAKNYPQVTTS
                     QYHIDGLTIQMVLNPQRFDVIVASNLFGDILSDLGPATAGTIGIAPSGNINPERTFPS
                     LFEPVHGSAPDIYGKNIANPIGQIWSGAMLLDHLGYPAAGVAIVNAIEQCLQDGPRTP
                     DIGGKAKSEDVGRAIASVF"
     gene            17028..18107
                     /locus_tag="VN496_03550"
     CDS             17028..18107
                     /locus_tag="VN496_03550"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HXF16050.1"
                     /translation="MKTYTIAAIPGDGIGTEVIGAGIEALETLAKRDGDFALRFEHFD
                     WGSDYYRRHGEMMPADGLSRLQPFDAIYFGAVGSGEVPDHISLWGLRLNICQSFDQYA
                     NVRPARVLPGINPPLKLASPADLDWVIVRENSEGEYSGQGGRTHRGLPEEVATEVAIF
                     TRTGVERIQRYAFELARKRPRKRLTLITKSNAQRHGMVMWDEIFARVATDFPDVQTDK
                     MLVDAATMRMVLRPSSLDVMVATNLHADILSDLAAALSGSLGIAPTANLNPERRFPSM
                     FEPIHGSALDIAGRGIANPIGTFWTAVMMLQHLGEMRGAARLMGAIEHVTSSRIFTPD
                     LGGSATTRQVTEALCRAIAASPDHA"
     gene            18290..20200
                     /locus_tag="VN496_03555"
     CDS             18290..20200
                     /locus_tag="VN496_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF16051.1"
                     /translation="MTSSKKLLSTSRRNTKTASDNQPRPGHSEAEQRIADLERELAQV
                     QFQLAAANIKLESAKASVPDAGSADPEEMNPGTLEALKASLDRKRLALAFAEIGTCEF
                     DVKTSVVHSSEMSLRLFGIRTNQRDVPYDTLLACLHLEDRPKFDHALEQCLNGTGNLD
                     IEYRVSWTDGNYRWLRTKGDALLNLDGEPIKVLWVTEDVSQRKDMDARVRFLAHHDLL
                     TGLPNRTLFQDRLQQALAAAKRMQSRVALLFIDLDRFKYVNDSFGHRAGDILLQTVAS
                     RLRKCVRETDTVCRHAGDEYLIVLSALRDPSEAALVAEKVLKIFDEIFVLENHEVQIS
                     ASVGISVYPDDGQTMEDLIRNADAAMYHSKKSGRNRFEFFTAELNAPVAERLVLANQL
                     RRAIENNELILHYQPQFDTTTSQLVGAEALVRWNHAEHGLLFPDSFIALAEESDIIHL
                     IGEWVLNEACRQIADWQVRNIKLVPVAINFSAFQFRRANLVENVAAAFARHGVKPHQL
                     EIELTENAIMQDPKETAKTLDQLNEMGVSLSIDDFGTGYSSLNYLKRFPIDKLKIDRS
                     FVEDLPHDLNDAAIVQAIINLAKSLRMIVIAEGVETRAQLDFLRSLSCEVYQGYLGGR
                     PIDAERFAALLK"
     gene            20475..21197
                     /gene="ompR"
                     /locus_tag="VN496_03560"
     CDS             20475..21197
                     /gene="ompR"
                     /locus_tag="VN496_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HXF16052.1"
                     /translation="MRETAANILVVDDDQRLRDLLHRYLGEQGFGVHAVGSAPAMDKV
                     MGKEPVDMIVLDLMLPGEDGMSVCRRLRAAGRRTPIIMLTARGDESDRIAGLDVGADD
                     YLPKPFNPRELVARINAVLRRQTLTPSPGAPEEHRKPVKFGPFEINLATRTLTRNAKQ
                     VGLTTGEFAMLKTLVTHPRVPLSRDRLMELARGREYGVFDRSIDVQVSRLRRLLEEDP
                     AHPRYVQTVWGVGYVFVPDDDN"
     gene            21245..>22336
                     /locus_tag="VN496_03565"
     CDS             21245..>22336
                     /locus_tag="VN496_03565"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXF16053.1"
                     /translation="MLLISALLLLSQLIWFGLYRTYNIRAQSEYLASQIASVISTVSV
                     ALETMPASARRKFSEKLPAQQNIRLLPATAWDDEEVPLPQSPLLKSISDHLKRDFSGD
                     AQALVMLEDATRALWIKIKVKDQAYWVVFPPESRGSASAWAWSGWSIFGLTLAMAGGY
                     LLMLRVNRPLQALASAAGEIGAGKTPAPLDENGPTEIRMLSHAFNQMTADLKRIDADR
                     AVLLAGISHDLRTPLSRLRLGIEMLVDTQHDGLKPGMISDIEDMDAIIGQFLDFAREG
                     GNEPSSAGEDLNHIVSSVCERFCRSSQVIRTQFGELPFMTMKPIAMQRMVTNLVDNAL
                     RYGGDRVEVTTEHVGGTVILRVLDRGPGIP"
BASE COUNT         4793 a         6479 c         6466 g         4598 t
ORIGIN      
        1 gcatatcgtg atccgccgta aatacgccgc cgtagcccac ggtatcgccc ggtttgagtt
       61 tttgaattgc aatgacttcg ctttttaaag tcatcactgg ttgcagtccg agtttttctg
      121 cgctgatatc gtgaagagga ctggcgccat agagcatcag ccccgggcgc acccaatttc
      181 catgcgcttc cgggaaacgt agcagagccg ccgagttcgc cagtgaacgt tcgtaatccg
      241 ggcgggcaac gcatgaattg aattcgcgca tttgcgtttg gataccgccc ggtccttccg
      301 cgtcggcaaa atgcgtcgtc agtacgattc gggaaataca cggcaacgat tttgtttgct
      361 cgatcacttc gcccgccttc gttaccggga acccaaggcg gttcatgccg gtattgaact
      421 tgaggaatac atccagctta tcctttgccg gcatggcttg tagcatgtgc agttgttcgc
      481 gtgcatgcac catgatggtt aatttgtgat gtaccgctac aggtaattcc tcctccgtaa
      541 agcagccttc cagcagcaaa atacgctgcc ggtaccccgc ttcgcgcaag cgaaccgcat
      601 cgtcaagttc cagcagcgcg aacccatcag cgtctcccag cgccttggcc accctcagca
      661 gtccgtgacc gtacgcgttc gccttgatta cggccattgt tgaagcgccc gcagcaagcc
      721 ggcgggcaac ggcgagattg tgtttcagtg cggcaaggtc aaaagtagcc tgaatcgggc
      781 gggccatgta tggtctgtat tccggcgatg ggaatattta ttgaaaatgc tgtgtcgtca
      841 ttctatgcgc tcggccacaa gatcaggcag ttcgattacg cgattgcacg atgagggaac
      901 attattgtgg tataaagcgc ccggctgatg atgcaatgat cgctaacgaa aagaccccgg
      961 attcaccgct gccgcccgct ccgataccat gaaacggggg ttctacacta tcctggccgc
     1021 gcagtttttt tccgcgcttg ccgataatgc gctgctcttc gccgcaattg cactgctggc
     1081 gagcttgcat gcacaagcat ggcagactcc ggttctgcag cagttcttcg tttttgccta
     1141 tatcgtgctg gcaccttttg tgggtccgtt cgcggattct ttgccgaagg gtcgcgtcat
     1201 gttcgttgcc aacgcggtca agttgctggg ctgcgttgcc atgctgatgg gcacgcatcc
     1261 tttgctcgct tacggactcg tgggcgtcgg tgcggccatg tattctccgg cgaagtacgg
     1321 catcctgacc gaatacctgc cccatcagaa actggttctc gccaacggct ggatggaagg
     1381 actgacggtg gccgctatca ttctcggatc cattctgggc ggcatcatgg tcagcgctcg
     1441 attcgccgga tacattttct cacgcatcga tttcccgctt gtcgaaaccg gcattgacac
     1501 tccgccggaa ctcgcgatca cggtaattct gatgttgtac tttagtgctg ccgtaatcaa
     1561 tctttacatt cccaagctgg ccatcgacca caaactgcaa aagcgtaatc ccgtgttcat
     1621 catgcaggat ttctggcaca gcttccggtt gctctggcgc gatccgcttg gtcaggtatc
     1681 gctcgccgtg acgacgcttt tctggggagc tggagcgacc ctgcggctga tcgtgctgac
     1741 atgggcatca atggcactga agttcagttt ggagaaggca actcaactga ccgctgtggt
     1801 tgcgtttggc atcgccctgg ggtctgtgct ggcggccaaa tatgtttccc tgaaacgcgc
     1861 cgtacgcgta ttgccggtcg gcatcgcaat gggagtgctg gttatgacca tggtgttcgt
     1921 gaatgactgg cgcatcgcaa tcgtgttgct ggtgcttatc ggagcaatgg gcggttactt
     1981 cgtggttccg atgaacgctc tgttgcagca tcgcggccac ttgctcatgg gggcaggtca
     2041 ttctatcgcc gtacagaatt tcaatgaaaa tttatctatc ctggttttgt tgggcgtgta
     2101 cgcgcttatg atcaaagtcg atgtgtcgat ccggggcatt atcattacgt tcggattgtt
     2161 cattgcggtc gccatgggat accttacccg caagcactgg cacgatcagg acagcaaatg
     2221 aatcccggca gtccgctata gcatcacggg ccgatcggaa agttcattgt gccccggacg
     2281 cgggtccgga aactgcgact ccagcaacgc ggaaacgcgc tcgattcctg tcagaacgcc
     2341 ctgctcaaaa tggccctggc gaaatgctgc ttccatttca cgacagaccg tctcccagcc
     2401 ttgggttccg acccgcgcat gaatgccgcg atcggcaata atttcgacat cgcgatccgc
     2461 caacaataaa taaatcagca cgccgttgtt gtgctccgta tcccagacgc ccaggtctgc
     2521 aaacacctgc agcgctcgct gtgcagccga aatgccccga acaagcgccg cggtgtccaa
     2581 tgcatgctcg acagcaaaac gaatctgacc gccatgcttc gtttcggatt ccgcaatcgc
     2641 gccctggata cggttcagcg cggctggtga aaacgaccgc cgcactatcc atgctggcga
     2701 catccaatgg cgaagcaggc gggacatgtt catatcacca acgtcccgaa gccccgccgc
     2761 cgccaaatcc tccaccgcca ccgctgaacc cgccgccgcc accaaagccg cctccgccaa
     2821 agccgccgcc gccaaaaccg ccaccgccat atcgtcccgg gattgatgcg ccggccgaca
     2881 aagtgacaag aaatgcgacg gcgcccacga caatcgcgat gatcagtggc gccacaatta
     2941 accaagccag gaatccaatt cctccgccaa cgatcagcgc cgcgggaagc cgccctaaca
     3001 ggtgtcgcaa caggccgccg aaggcgagcg tggccaccag gaacaacacg aagtaggatt
     3061 cgagcgagcg cagatcgccg ctgccgcggt ctgatacttt tacgtcggga agtggttccc
     3121 cgtcgataac tttgatcagc cggtctatcc ccgcattgat gccttcgtag aaatttccct
     3181 ctcggaaacc ggggatgaaa acttcatcga tcaagcgtcg cgccaccgca tccggaatga
     3241 caccctcgag tccataaccg acctcaaggc gcatgacgcg gtccgatttt gccactacga
     3301 tcactatgcc gtcgctgatc cctttacggc cgatcttcca ggcgtctgca acgcggatgc
     3361 tgtatgactc gattggctcc ggaaaggtgg tagcggtgat cagcacaacg atctggctac
     3421 ccttgtgttg ctcgaagtcc tgcaatttgc tctctaatgc gcgcttctgc tccgcagtca
     3481 gcgtacccgt ttcatccaca acacgaccgg tcagcgcagg gatgggccgg tcgtcagcca
     3541 agcaaatcag gctgaacaag aacggtacgg cgaaaatcgc cgctcggaca agcatggaca
     3601 ttgtgactac ttctttggct cgggtttcga gaaatcgacc gtgggtggct tggatatcgc
     3661 ctgctcgttt tcgaccgtaa aggaaggctt gggcttatag ccgaacacca ttgcagtgag
     3721 gttgcttgga aaggaccgga cggtaatgtt gtattcctgc acggcacgta tgtagcgctg
     3781 gcgggccacc gtgatacggt tttcggtgcc ctccagttgc gcctggagat cacggaaatt
     3841 agcatcggac ttcagttgcg gataattttc agcgacgacc agcaagcgtg acagggccga
     3901 tgtcatctcc ccttgcgccg ctacaaattt ggaaaatgcg gctgggtcat ttaccagttc
     3961 cggcgtggcc tggataccgc ccaccttcgc gcgcgcattt gtcacgccaa gaagaacgtc
     4021 tttctcctgc gcggcaaaac ccttgaccgt attcaccagg ttgggcacaa gatcggcgcg
     4081 ccgctgatac tgattaagca cttcgcccca agccgccctg atgccttcat cttgagactg
     4141 caaggtgttg taaccacatc cgttcagccc gaaggtcact gcaacaagca gccacagcac
     4201 gacgatgcga ttcattaaac tctctccccg aaaaaagaat ggcaagacta aggtgaggcc
     4261 aggcgctgtt gcttcaatgc cgccatggta tccacagcaa aacagaggtc ttcccaggac
     4321 tttgccttat cgaccagatt gcgcaacagg tatgccgggt gataagtcac gatcaacggc
     4381 gtgccatgat agtcgtgaac ccttccgcgc aggcttgcga gcgttgcgtc aatgcccaaa
     4441 aggttctgcg cggccaccct gccgagtgcg atgaccagac gcgggcggat cagttcgaac
     4501 tgacggcgca gataagcgtc gcaagcctgg gcttccacgg gctctggcgt acgattgccc
     4561 ggtggccggc atttcacgat gttcgcgatg tacacgttct tgccacgctg caatgctatc
     4621 gaggcgagca tgttatcgag gagcctgcca gcctggccaa cgaacggttc gccgcgctca
     4681 tcctcctcgg cgcccggagc ttctcccacg agcagccact ctgcttttgt gtcgcccacg
     4741 ccgagcacgg cttgcttgcg tcccttgtga agcgcgcatg cggtacaggc agatgcggaa
     4801 atcttgagct gcgcccaatc catttccaga attttcgcca ctcgatcggg acccgcccct
     4861 gcgcgacgag catcatggcc gctgactgga gtaattggca catccacatg tgagtcagat
     4921 ggtttgcggc gcacccatag cggcgacagc ccgagttccg cgatgatgaa gtcgcgtcgg
     4981 ctcaagttat ggagctcaca gggatatacc catcaggatc gcgtcctcac ggccgcggcc
     5041 ggctggataa taaccgcgcc gaaccgtgat atcacggaaa ccaaatccga tataaagctg
     5101 gcgcgcagcg ctgttggaag gacgaacttc cagaaacaat tgtgccgcat cgctggcacg
     5161 cgcaatattt atgaaatgct ggaggagcat gcggccatag ccgcggcgtt gccacgccgt
     5221 tgctacactg agatttagca ggtgcgcttc ccgcacaccg atcatcagta cgccgtagcc
     5281 ggccagtgtc cgattgcatt cgacaatcca gcaactgtat ccagcagtca gcgaatcgct
     5341 gaagttccca cgcgtccacg ggtgcgaata aagcgcgggt tccaggtcca ttacccggtc
     5401 gagatccgta gccagcattg gacggaattc aggcagattg tcgagttgcg cgctcatcgc
     5461 tgttcatgca ttttaagcgc gaccttgtca cgcacataga ggggaagtgc ctgttcggca
     5521 ggcacgcctc cacccgcagc cagcaccggc atggccagtc gcgcgatcga tcgcgcatgc
     5581 ggatgcagct ttgcttcgac acgcacaagc tgtccgccat agcgctttgc cagcgcgtcg
     5641 ccgtaaacgg cgaatccatt gccacatcca agccagccat cgccttccag cacaggtgcc
     5701 gaggaggcag ggcccacagc gggacacgtc atttccgtcc agcctttgtc atcatcacgc
     5761 cggtacgacg catgatagat ctcacccatg cgggcatcca ggcacgccag cacccgcgta
     5821 ccgcccgctt cctgggccaa ggcagccaac gtcccgaccg gaaccaccgg aagttcagca
     5881 ccgaacgcaa gtccctgcgc gacaccgcac gcgattctga tgccggtgaa ggaacccggc
     5941 ccggcaccga aagcaatgcc gccaagctgc cccaccgcca ttccggcagc acgtaacaac
     6001 tgctcgatca tcggcagtat aagttcggaa tgccgttgga cggcgtgcgt ctcgagataa
     6061 gtgagttctc cgtcgcgcat cagcgcgacc gaacaatatt ccgttgaagt atcgagagcg
     6121 agaagattca atgctggtcc gctggtctcg ggacgcaatg atgcttgtgt gacaactgat
     6181 tttagctgaa gcgagaacgc cgcgtttagt tggttataat tggaagagca tcgtggccgg
     6241 tccacaacgg ccggtaccgc aacaatgatc agggttgcac cggcttcgcc agtcatcaca
     6301 caaaacaata atgtgcacat cccgaggact ttcatgaccc gttttgcgcc ggctcggtgc
     6361 aatgctcgtt tgttttcgtc ctcgtgtttg gcaataaccc gatggcatcc taagacacgc
     6421 cttttcacat atcgacgtga tggcctttct atcctgggac ggtagcaggc ccccgatgca
     6481 atgaaccagc gtggacttgg gttacggatt tcaaaactca tggagcccgc ttggcaggcg
     6541 caaggacaaa gtgtgacggg aacgatatcg ggctcatttt ggagttaaac attcttcatt
     6601 ttggggttca ttgacatgga actatctgta aagggtcgcc gcgtgctgat tacagccggg
     6661 gctggcggca ttggccgggc gatgaccgac gtgttccacg ctgcaggcgc ccgcgtgcat
     6721 atctgtgacg tggttcaggc gtccatcgcg gaaacccaac gggcctttcc gggcgtgtcc
     6781 gggacactgg ccgatgtatc aaggctcaag gacgtagatc aactttttgg agaagttaga
     6841 cagcacctcg gtggactgga ttgcctgatc aataacgccg ggattgccgg gccaaccgga
     6901 aaagtggaag acatcccggt cgaggaatgg gagcggtgca taaacgtgga catgaacggc
     6961 atgttttact gcactcgcct cgccatgcca ttgctgaagg cggctggtgg tggctccatc
     7021 atcaatttgt cttcggccgc ggggcgccta gcgtttcctt tccggacacc gtattccgcc
     7081 gctaaatggg gtgtcgttgg ctacaccaaa agcctgtcca tggaagtcgg gcctgacaac
     7141 atccgggtca atgccatcca gcccggagtg gtcgagggcg agcgcatcaa tcgtgtgatc
     7201 gaagcaaagg ccaaggctct tgggatcagc ttcgaggaac agaagaagat ttccctcgaa
     7261 cgcgtgtcca tgcatcgcat ggtcagcccc tacgacatcg ccaacatggc gctgtatctt
     7321 gccagtgatg ctggccggaa catcaccggc caggcgattt cagtctgcgc cggcgtggaa
     7381 atgctgggat aacaataatc gacgacgaga acaacatgga aaaagtagcg atcattggtt
     7441 gcggtctcat cggccgcgcg tgggcgatgg tgttcgcgcg cgcgggccat tccgtcaaac
     7501 tttacgacgc cgctcccggc tctgcgcaac aagcgctctt gctaattcgg caaggactgg
     7561 agcaattgca ccagttcggc cttatcgcgg aatcggcaga catcgtgctc agccgcatga
     7621 gcacggtcac cacactggcg gacgcagtga gcgatgcgga ttatgtgcag gagaacaccg
     7681 ctgaaaaact ggatgtgaaa cgcgaggtgt atcggcaaat ggacgcagcg gcaccggccc
     7741 attgcattct cgccagctca acttcgacga ttcagacatc gcgcttcagc gaaagcatca
     7801 agggccgcca tcgttgcatc gtggcgcacc cggtaaatcc gccgcacatt gtgccggtcg
     7861 tggaaatttc gcccgccccg tggacgagcg cggaagtcgt ggcaaagact cgcgcgctgc
     7921 acgagaaagt cggccaagtc gccatcaccg tcaagaaaga agtcgaaggt ttcatactga
     7981 accgcttgca agccgctttg ctgctggaag cgtggcggct ggtaaaggaa gattacgttt
     8041 cggtggaaga cttggacaaa tgcatcaagg acggtttggg gttgcgctgg tcattcatgg
     8101 gcccattcga gaccatcgac ctcaatgcgc ccgggggcgt acctgactat gccaaacgct
     8161 atggcccggt gctgcacggc atgatgagca acacgaagaa cgagcaatgg gatgaagcgc
     8221 tgatcgccaa agtcgacggt gagcgccgca aaatcatgcg gcaggacaga catgccgaac
     8281 gtgaggcctg gcgcgaccgc cggctgatgg ccttgattgc gcacaaacgc gaggcggcca
     8341 agaaaatagg aaactgaacc gggttacggg agaacgaacg tggcaaacaa agtcatcatc
     8401 acttgtgccg tgactggcgc aattcataca ccttccatgt cgccgcactt gcccatcacg
     8461 ccagatgaaa ttgcccaggc ggccattggc gccgcaaaag ccggcgcagc agtggtgcac
     8521 ttgcatgcgc gagaacccaa ggacggacgt ccgacgcagg atccgaagat gttcctgcag
     8581 ttcctgccaa aaatcaaagc tgcttcagac gtggtgatca atctcactac gggcggcgcg
     8641 ccgacgatga gcattgaaga ccggctgcaa cccgcattgc aactgaaacc ggaagtcgct
     8701 tccctgaaca tggggtccat gaactttgga ctgtacgaaa tgatcccacg cgtcacgcaa
     8761 tggaagtacg aatgggaaaa accgtacctt gcgggctcgg atgaccggat ttttagaaac
     8821 acgttcaagg acatcgccca tattctgcaa tcgtgcagca acaatgacac ccgcttcgag
     8881 atcgagtgtt acgacattgg gcacctttat acggcagccc atttccttga tcgcgggctg
     8941 gtcaagcctc ccctcttcat ccaatctgta ttcggaattc gcggcggaat cgggccgcac
     9001 ccggaggatg ttctgcatat gaggcgcacc gctgaccggt tgttcggcga tcagtatcag
     9061 tggtcggtac tcggcggcgg aagaaaccag atgtacatcg caactcaatc ggcagttatg
     9121 ggcggtaacg tccgggttgg tctggaggac agtctgtgga tcggcaaagg acagctggcg
     9181 aaggccaatg ctgaccaggt ctctaagata cgacgtatcc tggaggaact cggcctggaa
     9241 gtagcaaccc ccgaagatgc gcggaacatt ctcaagctaa aaggcaagaa caacgttaac
     9301 ttctaaaggc gttcaggttc ggcgcttcgg gtgaaaaaca atcaccccgc gccctctaca
     9361 cagatgtata caattcggcc ttaccaattc atcgacacca tgccagcgtt tgatgctatg
     9421 gggagcggat tgtggccgac caggtgatgc ggcaacgcct tgctgcgcta cttgccgctg
     9481 acgtggcggg ttattcgcgc ctcatggcta ccgatgagcg cgcgacggtg gaggcgctgg
     9541 acgcagggcg cgcgatattc cgtagactga tagaggccaa tcagggccgc gtaatcgaca
     9601 tggccggtga ctcggtactg tccatttttg aaacggcggg tggcgcggtc agcgcggcgt
     9661 tgtccattca gaaagaactg tcatcggcat cctgctcgct accggaagac cgcaagatgc
     9721 gctttcgtat cggtgtgcat ctgggtgatg tcatcgaaaa agccgacggc accatctatg
     9781 gcgacggggt aaacatcgcc gcgcggcttg aagcgcttgc ggacccgggc ggcataacgg
     9841 tctcggatgc cgtccaggtc gcacagcgca accggcaacc ggtggtgttt gaagacctgg
     9901 gggaacacga cgtcaaaaac atcgcgcatc ctgtacgcgc attccgggtg cgcagcatcg
     9961 gggatgcacc cgaagcaggg ccgacatcgc cacgatttgc gctgccggac aaaccgtcga
    10021 ttgcggtgct gccgttcgac aacatgtcgg gcgatccgga acaggagttc ttcgcggatg
    10081 gcgttgtaga agcaatcacg gcaacgctat cacgtattcg ttcctttttc gtcatcgctc
    10141 gcaattcggc gttcgtctac aaaggcaagg caatcgacgt gcaacacgtc ggtcgggaac
    10201 tgggtgtgcg ctacgtcctg gaaggcagcg tgcaaaaatc cggcactcgc gtgcgcataa
    10261 cggtgcaatt gatcgatacc tcaaccggcg cgcatgtctg ggccgaccgc gtggatggca
    10321 cgctggagga cactttcgat ctgcaggacc ggatcaccga gcgggtcgcc ggtgcgctgc
    10381 agccatccat acggctcgca gagatagaac gcgcgcgacg caagcggcct caggagctgg
    10441 gcgcttacga ctacaccatg cgcgcgatgc cgtacgtgtg ggtactcgaa aaggacgcaa
    10501 gcgcccaggc gctcattctg cttggagaag cgcttgcgat agacccggag taccccatgg
    10561 cgctctccct cgctggctgg tgtcatgcac agcgcctggt ttataactgg accgaagaca
    10621 ttgatgtgac gcgacgtgaa gcgcttcggg gtgcggagac agctgctcaa catggtggcg
    10681 acgatccgct cgtgcttgcg gtgctcggag ccgtgcacac gattctgcgc aacatgggga
    10741 ccgcccggat catgctggaa cgcgccgtgg cgctcgaccc taacgccgca tgggcatggt
    10801 cccggctggg ctggatcgaa aactattcgg accgtccggg tcacgccatc gaacatttcg
    10861 aacgcgcgct tcgcctcagt ccgctcgatc ccatgaactt caacaattat gtcggcatcg
    10921 cttcagccca cgaaatagcg gaacgctacg atgaggcggt agcgcaatac cggcgcggat
    10981 tgcaggaacg gccccatgcg cgatggatcc tgcgcaacct ggtttcgtcg cttgccgggg
    11041 cgggccgtat cgaagaagcg cgcgaggaat accttcaatt gtgcgaggag tatccgggtc
    11101 tcacggccgc gaagttccgc aacgccatgg ttttttcgcc ggccgttctg gatcgcatga
    11161 ttgccaacct gaaaaaagtg ggattacccg actgaaccac agcgaacgag ccatggcttc
    11221 tgaaactgcc ctgctggaca cgccattgcg cggcctgcat gtcgaactcg gcgcaaaaat
    11281 ggttgccttt gccggctatg cgatgccggt gagctattca accggcatca tcgcggaaca
    11341 tcggcattgt cgcgcggccg ccgcactgtt tgacgtctcg cacatgggac agttgcgggt
    11401 gaccggtgcc gatgctgccg gagcactgga atcgcttatg cccgccgata tcgttgacct
    11461 cggggtgaac agacagcgct acggtttctt tactaacgca agcgggggca ttcttgacga
    11521 cctgatggtg acgcgccgcg aggatgacat attcatagtc gtgaacgcgt catgcaagtc
    11581 agccgatatc gcacacctga ccgcgtgcat tggccagcgg tgcagcgttg aggcgctgcc
    11641 gcaactcgcc ctgctcgcgc tgcaaggtcc acaggcggcc gttgcgctat cgcgattgaa
    11701 tgccgcggtt ggaaaactgg cgttcatgac cggcatgcgt gccaggctaa acagctcgga
    11761 ctgcttcgtc acgcgctctg gatataccgg tgaagatgga tttgagatct ctgtggaagc
    11821 ccctgctgcg caaacgctgg cccgtgtgct gctggcacag ccggaggtca aaccggctgg
    11881 cttgggcgcc cgcgacacgc tgcgcctcga ggccggcttg tgcctgtatg gccacgacat
    11941 tgaccaaacg attacaccgg tcgaagcagg cctgacgtgg gcagtcgcga aggttcgccg
    12001 tgctggcggc gttcgagccg gtggctaccc gggggcaaca gtgctcgaga atcaaatcgc
    12061 gcggggctgt gcagtgaaac gggtgggatt gaccgggtcg cagcgcatac cggtgcgcga
    12121 aggcgcaatc atcactgatc tgcagggagg caagttaggc aaggtcacca gcggtacgct
    12181 gtcgccaagt ttgaattacc caatcgcgat gggttatgtc gcgaccgata aagcgaacgt
    12241 cggccagaat ttgttctcgg aggtgcgggg caagattcag ccaatgggcg tgaccacgat
    12301 gccttttgtg ccgcaccgct atcatcgcgt gtagttcagg tacgtagcca ggaagcacgt
    12361 tcccaggccg ccatgccggc ggcccgtagg ccgagacatg aggagcagtc gatgacaacc
    12421 aggtacaccg cagaacatga atggatcaat cgcgatgacg atgctgcagc cactgtcggt
    12481 atcacccagc atgcccagga cgcgcttggg gacattgtgt tcgtcgatct tcccaccgtc
    12541 ggcaccgcct acaagaaagg cgacgttgca ggcgtcatcg aatcggtgaa ggcggctgcg
    12601 gacctgtaca tgccggtcag cggcgaagtt gtcgaggtca acgaagcctt gcgtaatgat
    12661 ccctccctgg ccaatagcga cccgcacggc gcaggctggt ttttcaaaat gcgcgtcaac
    12721 gacaaggtcg aattagagca attgctggat gcggcagcgt atgacgcgct ggtcaagagc
    12781 gtctgacctt tccagccata gcgtaacaag ttcctcgtaa tgtccttcta ccagccactc
    12841 gacccgctcg acacacattc cgagttcgtt gcccgccacg ttggcatcga tcaggaagac
    12901 gagcggcata tgctagagac aatcggcgtg ccttccagaa ctgagctgat caaggccatt
    12961 gtcccgggca gcattgctcg caagaaacca atgcggctgc cggagcctgt gggtgaggcg
    13021 caggcgctgg cggaattgaa aacgattgct ggccacaata gcgtactgaa gagttacatc
    13081 ggccagggct atcacggtac atacacgccc ggcgtaatct tgcgcaatgt gctggaaaac
    13141 ccggcctggt acaccgccta caccccgtac caggcggaga tctcgcaagg tcgcctcgaa
    13201 gccatgatga ttttccagac catggtatgc gacctgacgg gattgccgat tgcgggtgcg
    13261 tcgcttctcg atgaagcgac cgctgccgcc gaggccatgg cgctcgccct gcgcatgact
    13321 acaacacgtt caaaaacttt cttcgtagcg gacgatgtac tgccgcaaac gctcgaggta
    13381 ctgcatacgc gtgccaaacc gctgggcgtt acgatcgtga ccggtgccgc gcgggatgcc
    13441 ggccgcgccg aagcgtttgc cgtgctcctg caatacccgg gagtcaccgg cgaagtgcat
    13501 gacctcaagc cgttggtgga ttccgctcat cagcgtgaag ccttggtcat tgtggctgcg
    13561 gacctgcttg caatgacgct gcttgtttcg cccggtgatg tgggagccga catcgcggtc
    13621 ggcaatacgc agcgcttcgg catgccaatg ggcaatggcg ggccgcacgc ggcgtatctg
    13681 gccaccagag aggcgttcaa gcgttccttg cccggccggc tggtcggtgt cagtgttgac
    13741 atgcatggag cgcccgctta ccgattggca ttgcagaccc gcgagcagca tattcgccgc
    13801 gaaaaagcga ccagcaatat ttgtacggcg caggtattgc cggctgtggt cgcaagcatg
    13861 tacgcggtct atcatgggcc ggacggcctt cgacgcatcg ccaagcgagt cgcgagctac
    13921 gcggcgattc tggctgccgg tgcaaggcac cttggttgcg acattgtgca ttcgaccgga
    13981 ttcgatacgg ttcaatgcac taccggcgcg cgcaccgaag ctgtgctgca agccgcgcgc
    14041 gagtgcggct ataacctgcg ccgcagtagc cccaccacta tcggcataac tttggacgaa
    14101 accacgacga gcgaggacat cctcgcgctg tggccggttc ttgctcatgc cggcaacgcc
    14161 acccgcttgg cgcaggtttc caagctgagc gaactttggg ctgaattgcg taaagatgtt
    14221 gcgccgctgt ttcctgcctc attcgcccgt gccagctcat tcctgacgca tcccgtgttc
    14281 aaccgtcatc acaatgaaac ggatatgctg cgttatttgc gcagccttgc cgataaggac
    14341 ctcgcactcg accgcagcat gattccgttg ggatcgtgca cgatgaagct caatgccacg
    14401 agtgaaatga tcccgatgac gtggccggag tttgccaacg tacatccctt tgctccgcct
    14461 gagcagctcg ctggatacgc gcagttgcgt gagcagctcg aaggctggct aggccaaacg
    14521 accggctacg acggcgtgag tctgcaaccc aacgccggct cgcaaggcga gttcgctgga
    14581 ttgctcatca tcaaggcatt ccaggaatcg cgcggccaaa cccagcgcaa tgtttgcctc
    14641 attcccgaat cggcccatgg taccaacccg gccagcgcac aaatggctgg gctaagagta
    14701 gtcgtcacgc gttgtgatgc gcgaggcaac gtagacctcg cagacctgcg cgcaaagtgc
    14761 gagcagttca gcgacacgct ggcggcggtg atgattacct atccctcgac ctacggggtg
    14821 ttcgatccgc agatcacgga aatctgtgcg ttggtccacc aatatggcgg acgcgtctat
    14881 gtggatggtg cgaacatgaa tgcattggtt ggcctcgcgg cgccgggcga attcggtggc
    14941 gacgtgagcc atctcaattt gcataaaacc ttctgcatcc cccatggcgg gggtggcccc
    15001 ggtgtagggc cggtgtgcgt agtctcagac cttatcccct ttctgcccgc acaccgtacg
    15061 gcggggatgg ccgggaattc acccgtcggc gccgtatcag ccgcgccgtt gggtaacgca
    15121 tccgtcctgc cgatcagctg gatgtacatt cgcatgatgg gggcacaagg cctgcagacg
    15181 gcctcggaga tcgctatcct gtcggcgaac tatgtcgccg cacggctggc gccgtactac
    15241 tcagtccact tcagcggcaa catcgccgac atcaagggcg gcggtgtcgc gcacgaatgc
    15301 attctggatc tgcgaccgtt gaaggactcc agcggcgtca cggcggaaga cgtcgccaag
    15361 cgacttatcg actttggatt ccacgcgccc acgttgagct ttccggttgc gggcacgttg
    15421 atggtggagc cgaccgaaag cgaaccgctg cgtgaactcg accgtttctg cgaagcgatg
    15481 atttcgatcc gcgccgagat tggcagagtt gaatccggcg agtggccgca ggatgacaat
    15541 ccgctaaaga atgcaccgca cacggccgag gcattgctca aagcggaatg gctgcacccg
    15601 tacacgcgcg aacaggcagc cttccccgac actgcgttgc gcaaatcgaa atattgggtt
    15661 ccggtagctc gcgtcgacaa cgtatgggga gatcgcaatt tgttctgcac ctgtcccccg
    15721 ctggatgact accaacagtg acatcaccag attggctgat ctgtccgcgc aaccggattg
    15781 cgtcgcggcg gcatttgcgg atcggtcaag tgccgtagca gaatgtcgcc cggcgctagt
    15841 cgcgccaggg caggttctgc agtgagggat attccatgac acaaaaccac aagatcgccg
    15901 ttatcgcggg agacggtatc ggcaaggaag tgatgccgga gggcgttcgt gtgctggagg
    15961 ctgcggcacg gaagtttggc attgcgctca cctttgacca tttcgactgg agctgcgaca
    16021 actacgacag gcacggctgg tggatgccgc cggactggcg ctccaggatc gaacgccacg
    16081 attgcatttt ctttggcgcg accggctggc ctgcgaaggt accggatcat aagtcgctat
    16141 gggattcgct cattttgttc cggcgcggct tcgaccagta cgtgaacctc cggccggtgc
    16201 gtctgatgcc gggaatcacg tccccattgg caggccgcaa agtcggtgac ctggattatt
    16261 atgtggtgcg cgaaaacacg gagggtgaat attccgccgt tggtggccgc ctgttcgagg
    16321 gaaccgagcg cgagatggca atgcagcagt cgatctttac gcgcaagggc gtcgaccgga
    16381 tcatgcggtt tgcgtttgag ctgtcgatga agcgcgctcg caaactgctg acgtcggcga
    16441 caaaatcgaa cggcatctcg atcaccatgc cttactggga cgaacgtttt gccgcaatgg
    16501 caaaaaatta tccgcaagta acaaccagcc agtatcacat cgatggactt acgattcaga
    16561 tggtactgaa cccacagcgc ttcgacgtta tcgtggcatc caacctgttc ggggacatcc
    16621 tgtccgatct tggccctgcg acggccggaa cgatcggtat cgcaccttcg ggcaacatca
    16681 atccggagcg cacttttcca tcgttgttcg aacccgttca tggctcggcg ccggatatct
    16741 acggcaaaaa catcgccaac ccgatcggcc agatctggtc gggcgcaatg ttgctggacc
    16801 accttggcta tccggccgct ggcgtggcaa tcgtgaatgc aatcgagcag tgcctgcaag
    16861 atggcccgcg tacacccgat atcgggggca aagcaaaatc agaagacgta ggaagagcca
    16921 ttgcttctgt gttctagact aaccatcaca cgaccatcac gataaaaagg caccgcaaaa
    16981 ttggccaact cgttttgcgc tattttcttc acgggtaaat gaactgaatg aaaacctaca
    17041 cgattgcggc aattcccggc gacggcatag ggactgaagt tatcggggcc gggatcgaag
    17101 ccctggagac cttggcgaaa cgggatgggg acttcgcgct gcgtttcgaa catttcgact
    17161 ggggcagtga ttattaccgg cggcacgggg aaatgatgcc agccgatggg ctgtcccggc
    17221 ttcaaccgtt cgatgcgatc tatttcgggg ccgtgggctc cggcgaagtg ccggaccaca
    17281 tatcgctttg gggtttgcgc ctgaacattt gccagagctt cgaccagtat gccaatgtgc
    17341 gcccggcacg cgtactgccg gggattaatc cgccgctgaa attagcttca cctgccgatc
    17401 ttgattgggt gatcgtgcgg gaaaattccg aaggggaata ttccggtcag ggcggtcgaa
    17461 cccatcgcgg tctcccggaa gaagtcgcca ccgaggttgc cattttcacc cggaccggcg
    17521 ttgaacgcat tcagcgatat gccttcgagc tggcacgaaa gcggccccgg aaacggctca
    17581 cgctgatcac gaaatccaat gcccagcgcc atggcatggt gatgtgggac gaaatttttg
    17641 cgcgcgttgc aaccgacttc ccggacgtgc aaaccgacaa gatgctggtc gatgcagcga
    17701 ccatgcgcat ggttctcagg ccatcatcgc tggatgtcat ggtcgcgaca aacctgcatg
    17761 ccgatatcct gtccgacctg gccgctgcgc tgtcaggcag cctgggcatc gcgcccacgg
    17821 caaatttgaa tcccgagcgc agatttccat ccatgttcga acccatccac ggttcggctc
    17881 ttgacatcgc cggcagggga atcgccaatc cgattggcac tttttggaca gcagtgatga
    17941 tgcttcaaca cctgggcgaa atgcggggcg ccgcgaggct catgggcgcc attgaacatg
    18001 tgaccagttc gcgcattttt acgccggatt tgggcggcag tgcaacgacc cggcaagtaa
    18061 ccgaagcgtt gtgccgtgcc atagccgcgt cgcccgatca cgcctgaacc aggtgcaccg
    18121 aaaccttgcc ggaaccgctt ccgcgacgga ttgcgggttg cctgcaggct catattcgaa
    18181 cagaatgtga ctggcatggt gcttgcacga ttcactggtc attttatgta ttttttgtgc
    18241 tgtcgatgac actgcacata gtcaacattc aaaccttgct tccgaattcg tgacttcttc
    18301 aaaaaagctc ctgtccactt cccgccgaaa taccaagaca gcgtcggaca accaaccccg
    18361 accagggcat tccgaggcgg aacagcgcat cgcagacctg gaacgtgagc tggcgcaagt
    18421 gcagtttcaa ctcgctgcag cgaacataaa actagaaagc gcaaaagcca gtgtgccgga
    18481 cgccggttca gccgaccctg aggaaatgaa ccctggcacg ctggaagcgc tgaaggctag
    18541 cctggaccgc aagcgactgg cattggcatt cgcggaaatc ggaacttgcg agttcgatgt
    18601 caaaacatcg gtcgtacatt cttccgaaat gagtttgcga ctgttcggca tccgcacgaa
    18661 ccagcgtgac gtcccgtatg acacattgct ggcgtgcctg cacctggaag atcgaccaaa
    18721 gttcgatcat gcgctggagc agtgcctgaa cggcaccggg aatctggata tcgaataccg
    18781 tgtgagctgg acggacggca actaccgctg gctccgcacc aaaggtgatg ctttactgaa
    18841 tctcgacggg gagcccatca aggttctttg ggttactgaa gatgtttctc agcgcaagga
    18901 catggatgcg cgggtgcgtt ttttggcgca tcacgatctg cttaccgggc tgccaaaccg
    18961 cacgctgttc caggatcgtt tgcagcaggc gcttgccgcc gccaaacgca tgcaatcccg
    19021 tgtcgcattg ctcttcatcg acctcgatcg ttttaaatat gtaaacgatt cgttcggcca
    19081 tcgcgccgga gacatcctcc tgcaaacggt cgcatcaagg ctgcgcaaat gtgtgcgcga
    19141 aactgacact gtatgcaggc acgccggaga cgaatatctc atcgtactgt cggctctgcg
    19201 cgaccccagc gaagccgctc tcgtcgcgga aaaagtcttg aaaattttcg atgaaatatt
    19261 tgtcctggaa aaccatgaag tccagatttc ggcaagcgtc ggcatcagcg tttacccgga
    19321 cgatgggcaa accatggagg acttgatccg caatgcagat gcggcgatgt accattccaa
    19381 gaaaagtggc cgcaaccggt ttgagttttt cacggcggaa ttgaacgcgc ctgttgcgga
    19441 acgactcgtt cttgcgaatc agctgcggcg cgcgatcgaa aacaatgagc tcatactgca
    19501 ttatcagccg cagttcgaca ccacaaccag ccagctggtg ggggccgagg cgctggtgcg
    19561 ctggaaccat gccgagcatg gccttctatt cccggacagt ttcattgcct tggcggagga
    19621 atcggacatt attcatctga ttggcgagtg ggtgctgaat gaagcctgcc gccagatcgc
    19681 agactggcaa gtcaggaaca tcaaactcgt gccggttgcg attaattttt ccgctttcca
    19741 gttccgccga gccaatcttg tcgagaacgt ggcagccgcg ttcgcacgtc atggagtcaa
    19801 accgcatcaa ctcgagattg aacttaccga gaacgcaatc atgcaggatc ccaaggagac
    19861 tgcaaaaacg ctcgaccaac tcaacgaaat gggcgtcagc ctgtcgatcg acgatttcgg
    19921 caccggatat tccagcctta actatctgaa gcgcttcccc atcgacaaac tcaaaatcga
    19981 tcgtagcttc gtcgaagatt tgccgcatga tttgaacgac gcggccatag tccaggccat
    20041 catcaatctg gcaaaaagcc ttcgcatgat cgtgatcgca gagggcgttg aaaccagggc
    20101 acaactggac tttctgcggt cgctgagttg cgaggtctac caaggttatc tgggaggaag
    20161 accgatcgac gcggagcgct tcgccgcgct gctgaagtaa ctccgagatt cgtcccctgg
    20221 cagttaaatc ctgccatacc cgaacttccc gcgttcccgc ataagttttc ggctcgctgc
    20281 tacggcgaaa aacgttgaat tcagcgcgtc gcgcgttgcg ttgccgccga cagccgggca
    20341 gacgccaatc cagctcgtta agcgccagtc cggtttttgg atccccggct cacgattagt
    20401 gccatcctgg cctgcagttt tacgatcaga acctggtgcg tcataattaa gtcttgccgc
    20461 ctgcaatacc cgccatgcgt gagacagctg caaacatatt ggtcgtcgac gatgaccagc
    20521 gcctacgcga tttgctccac cgctatcttg gtgagcaagg ctttggcgtg catgccgtgg
    20581 gaagcgcgcc ggcgatggac aaggtgatgg gtaaggaacc ggtcgacatg atcgtccttg
    20641 acctcatgct tcctggcgaa gatggaatga gcgtgtgccg ccgcctgcgc gcagccggcc
    20701 gacgtacacc gatcatcatg ctaaccgcca gaggggacga atcggaccgc attgctggcc
    20761 tggatgtcgg cgcggacgat tatttgccta aaccattcaa cccgcgggaa ctggtcgcgc
    20821 gcatcaacgc agtgctcagg cgacagacgc tgacaccgtc ccccggcgct ccggaagagc
    20881 accgcaagcc ggtaaaattt ggtccgttcg agatcaacct ggccaccaga acactgacgc
    20941 gcaacgctaa acaagtaggc ctgacgacgg gcgaatttgc catgctgaag acgctggtca
    21001 cgcaccctcg cgtgccgctt tcgcgtgacc gcctcatgga gcttgcgcga ggacgggagt
    21061 acggcgtttt cgaccgcagc attgatgtgc aggtctcccg gctgcggcgt ttgctggagg
    21121 aagatcccgc gcacccgcgc tacgtccaaa cggtttgggg cgtcggctac gtgttcgtac
    21181 ccgacgacga caactaggtc gtttctggat gagctggatt ccgcagtccc tattatcccg
    21241 ttcggtgctt ctcatttcgg ccctgctttt gctgagccaa ctcatctggt tcggtctcta
    21301 tcgcacatac aatatccgtg cgcagtcgga atatctcgcc agccagatcg ccagcgtgat
    21361 cagtacggta agcgtggcgc tggagaccat gcccgcttcc gcgaggcgca agttcagcga
    21421 aaaacttccc gcgcagcaaa atatccgcct gttgccggcc accgcatggg acgacgaaga
    21481 ggtcccgctg ccgcaatcac ctttgctgaa aagtatcagc gaccatctca aacgcgactt
    21541 ctccggcgac gcacaagcgc tcgtaatgct ggaagatgcg acgcgcgcct tgtggatcaa
    21601 gatcaaggtc aaggaccagg cctactgggt tgtgtttccg ccggagtcgc gcggctccgc
    21661 ctcggcgtgg gcatggagtg gctggagtat tttcggtctg acgctcgcga tggccggagg
    21721 ttatctgctg atgttgcgcg tgaatcggcc actgcaggcg cttgcctccg cagctggcga
    21781 aattggcgcc ggcaagacgc cggcaccact cgatgaaaat ggccccaccg agatccgcat
    21841 gctttcgcat gccttcaatc aaatgactgc ggatttgaaa cgtatcgacg ctgaccgggc
    21901 ggtactcctc gccggcattt cgcacgatct tcgaacgccg ttgtcgcgct tgcgcctcgg
    21961 catcgaaatg ctggttgaca ctcagcatga tggactgaag ccgggcatga tcagtgatat
    22021 cgaagatatg gatgccatca tcggccaatt tctggacttt gcgcgtgaag gcggcaatga
    22081 accttccagc gctggggaag acctcaatca catcgtgtcc agcgtctgcg aacgcttctg
    22141 ccggagcagt caagtgatca ggacccaatt cggcgaactg ccttttatga caatgaaacc
    22201 gatcgccatg cagcgcatgg tgaccaatct ggttgacaac gccttgcgct atggcggcga
    22261 ccgtgtggaa gttaccacgg agcatgtcgg tggcactgtt atcttgcgag ttctggatcg
    22321 aggaccaggt attccc
//
LOCUS       DATMZQ010000112         5521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2504651, whole genome shotgun sequence.
ACCESSION   DATMZQ010000112 DATMZQ010000000
VERSION     DATMZQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504651"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VN496_03570"
     CDS             <1..655
                     /locus_tag="VN496_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXF16054.1"
                     /translation="TSDLADVAEFFVADFTSQHSIGEMAASALRTAPCEKFALAGLSM
                     GGYVAQEIMRQSPERVTKLALLDTRSRAELPEETERRQELMQLAQSGRNFTPVTNRML
                     PFLLHASRIDEAPLVEVIRDMAQRIGIEAYLRQQHAIIARPDYRTLLAEISCPTVVLC
                     GRQDRLTPLENSEQMAKSIPGADLVIVEECGHMSTLERPAQVNSALRTWLARSRASQ"
     gene            complement(671..1237)
                     /locus_tag="VN496_03575"
     CDS             complement(671..1237)
                     /locus_tag="VN496_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXF16055.1"
                     /translation="MRELDGLKVAVLVADGFEQVEMVEPKRALEQAGAQPKIVSPVQG
                     EVQGWNHFDKADKFKVDVPLDQARAEDFDALLLPGGVANPDQLRMKPKAVEFVRSFMA
                     SAKPVAVICHGPWTLIEADAVRGRTITSWPSLKKDLQNAGANWVDKEVVVDNGLVSSR
                     NPQDIPAFSAKMIEEFREGSHAGRMAHT"
     gene            1438..2433
                     /locus_tag="VN496_03580"
     CDS             1438..2433
                     /locus_tag="VN496_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXF16056.1"
                     /translation="MSQLRIGISGWRYEPWRGIFYPTNLAQRRELEFAARAFSSIELN
                     GSFYSLQHPRSYAEWYDETPADFVFSVKGPRYITHIRKLNQVEIPLANFFASGLFNLK
                     EKLGPLLWQFPPLLRYDEDRFDRFFSLLPRDTAQALALARRRDARMNGRARLAIDRNR
                     ALRHAIEIRHNSFACESFIKLLRRHEIALVVADTAGKWPYLEDITADFMYLRLHGDKK
                     IYSSGYTDAALDRWQQRIGNWMTGGQPRDATLTSSVTAPPRKSRDVYVYFDNDVKVRA
                     PFDADRLMQKLGLMRADTSFVFPDRRRLGKFKASPLGPDQRWRSRVQTTVRTKPV"
     gene            complement(2486..2989)
                     /locus_tag="VN496_03585"
     CDS             complement(2486..2989)
                     /locus_tag="VN496_03585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF16057.1"
                     /translation="MTRSAADSIAFILAAEADALAIARMSRDLIETGLGWSWTGARVL
                     RAIRDPEALVLVARDGRQLIGFAIMDFGEETAHLALLAVDPSHRRRSVGRRLFNWLRE
                     SAVTAGIATVKLELRAGNLEAQQFYRGLGFQEVGWTVGYYRQRETALRMAMSLREPAA
                     GNSPSVR"
     gene            complement(2986..3906)
                     /locus_tag="VN496_03590"
     CDS             complement(2986..3906)
                     /locus_tag="VN496_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HXF16058.1"
                     /translation="MIAHVFEPRFLLLYGFIAAAVFVHLRGRVRHGFTRQVTDHSTIF
                     APLNAVLYAFSAVPNRPRLEIERFPELARLRENWVTIRDEALTLFGAGYIRAAEGYND
                     VGFNSFFRSGWKRFYLKWYDEPLPSARAMCPKTVELVQSIPSIHGAMFALLPPGGRLV
                     AHRDPYAGSLRYHLGLVTPNDDTCRIFIDGEPYVWKDGEDVLFDETYIHYAENRSDMT
                     RLILFCDVERPLKTKLMKRFNDFVANTLVRASKTKNIEGEPVGVLNRVFNYVYRIRLL
                     GKRLKARSRFAYYALKYALIGGLFYWIFLA"
     gene            complement(4006..4509)
                     /locus_tag="VN496_03595"
     CDS             complement(4006..4509)
                     /locus_tag="VN496_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HXF16059.1"
                     /translation="MEHVEGITRIIQLAVAPVFLLTAIGTILSALNNRLGRIVDRRRV
                     LEERLRQAFAQSERSDQADVNELQLLASRISLIYHAIVLAIVCALFICMLVASAFLGV
                     FVAIDIARLIGTLFILAMVALIGSLWMLLREVFLAVKGGRHDILVKDLLKLTSTGRES
                     SDLDRRL"
     gene            complement(4544..5128)
                     /locus_tag="VN496_03600"
     CDS             complement(4544..5128)
                     /locus_tag="VN496_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16060.1"
                     /translation="MKSTGWLIWTLCAALGACASQPKEPPRIMMQGFSISAPKEKEKT
                     WVVAQQSPELTVIGKQGRFTGESFSMQTTIIKLPAFTSSDAMMRYVESSLRKEIDPKR
                     FRLFKLETSEQKVHQQTCALSRLEMAERTSSDGTASPVNTMLETMTLTCPHPQDPLRA
                     INMAYSHRHFPEDLDPQFAQEGAMLMQTLEFEPL"
BASE COUNT         1132 a         1602 c         1589 g         1198 t
ORIGIN      
        1 aacatcagat cttgccgatg tcgcagaatt tttcgttgcg gacttcacca gccagcacag
       61 catcggtgag atggctgcat cggctctgcg cacggctccg tgcgaaaaat tcgcgctcgc
      121 gggcctgtcg atgggtgggt atgttgccca ggaaatcatg cgtcagtcgc cggaacgagt
      181 gacgaagctg gcgttgctgg atacccgttc gcgtgccgaa ttgcccgagg agaccgaacg
      241 ccgacaggaa ctcatgcaac tcgcgcagag cgggcgcaat ttcacgcccg tgaccaaccg
      301 catgctgcct ttcctgctgc atgcctcgcg catcgacgaa gctccgctcg tcgaagtcat
      361 tcgcgacatg gcgcaacgca tcggtatcga agcttatctg cgccagcagc atgccatcat
      421 cgcccggccg gattaccgca cgttgctcgc ggagattagt tgcccgactg tggtgttgtg
      481 cggcaggcag gaccgcctca ccccgctgga gaacagcgag cagatggcaa agtcgattcc
      541 gggcgctgat ttggtcatcg tcgaagaatg cgggcacatg tccacactgg agcgcccggc
      601 gcaggttaac agcgctctgc gaacctggct ggcacgctcg cgcgccagcc agtaatccgg
      661 gctcggcaca tcaggtatgg gccatacgtc ctgcatggga tccttcgcgg aattcctcga
      721 tcatcttggc agaaaaggct ggaatatcct gcggattgcg gctcgaaacc aatccgttgt
      781 cgactacgac ttccttgtcg acccagttgg cgcccgcgtt ctggagatcc tttttaagtg
      841 acggccacga ggtaatcgtt cggccgcgca ccgcatctgc ttcgatcaga gtccatggcc
      901 cgtggcagat tacggccacc ggtttggccg aggccatgaa tgatctgacg aattccactg
      961 ctttgggctt catgcgtagc tgatctggat tggctacgcc tcccggcagc aacaaggcgt
     1021 cgaagtcctc cgcccgagcc tggtccagcg gaacatcgac cttgaacttg tctgccttgt
     1081 cgaaatgatt ccatccctgc acttcgccct gcaccggtga aacgatcttc ggttgggcgc
     1141 cggcctgctc cagcgcgcgc ttgggttcca ccatttccac ttgttcaaat ccgtctgcta
     1201 ccagtaccgc caccttgagt ccgtcgagtt cacgcatgtc gatctccttt gtttgtttgc
     1261 cgagcgcagg ctcaagcaac ccatgtgcct tcggatcaaa ccgggcacgc cggttgcgct
     1321 caggttgact acggtgggtt agtggcggat tgagcagttc gagccgttct aatcggtcaa
     1381 accggatcgc gagcggtgta gtttttacca gacgaagccg tatttttgcc aggtctcatg
     1441 agtcaacttc gcatcggtat ttccggctgg cgctatgagc cgtggcgggg tatcttctat
     1501 cccaccaact tggcgcagcg gcgtgaactg gaatttgcag cgcgtgcttt cagtagcatt
     1561 gaactgaatg gttctttcta ttcactgcaa catccgcgca gctatgccga gtggtatgac
     1621 gaaacaccgg cggatttcgt tttcagcgtg aagggtccgc gctacatcac gcacatccgc
     1681 aagctgaacc aggttgaaat accgctcgca aatttcttcg cttctggctt gttcaatctg
     1741 aaggaaaagc tggggccttt gctgtggcag tttccgcctt tgcttcgcta cgacgaagac
     1801 cggttcgatc gttttttctc gctgctgccg cgcgatactg cgcaagccct ggcgctggcg
     1861 cgccgacgtg atgccagaat gaacggacgc gcgcgccttg cgatcgatcg caaccgcgca
     1921 ttgcggcacg ccattgaaat ccggcacaac agtttcgcgt gcgaatcgtt catcaagctg
     1981 cttaggcgtc acgaaattgc gctagtcgtt gcggacaccg cgggcaagtg gccctacctc
     2041 gaagatatca ccgcggattt catgtacctt cggttgcacg gcgacaaaaa aatatactcg
     2101 agcgggtata cggatgcggc gcttgatcgc tggcagcagc gcatcgggaa ctggatgact
     2161 ggcgggcagc cgcgcgatgc aacactcact tcgagcgtga cagcgcctcc gcgcaagtcc
     2221 cgcgatgtct acgtttattt cgataatgat gtcaaagttc gcgcgccgtt tgatgcggac
     2281 cggctgatgc agaagctcgg gctgatgcgg gcggatacct cgtttgtttt tccggaccgc
     2341 aggcgccttg gaaaattcaa ggcttccccg ctggggccag accagcgttg gcggtcgcgg
     2401 gtacaaacta cggtgcgcac caaacctgta tgagccgtct cactgcgaca atgcagttat
     2461 catctttggc gcgatgcgcg cgaattcagc gcaccgaagg tgaattgccg gcagcaggtt
     2521 cgcgcagcga catggccatg cgtaacgccg tctcgcgctg gcgatagtag ccgacggtcc
     2581 agccgacttc ctggaaaccc aggccacggt agaattgctg ggcttccaga ttgccagcgc
     2641 gcagctcgag ctttactgtg gcgatgcccg ctgtcacggc actttcccgc aaccagttga
     2701 acaaccggcg gccgacactc cggcgacgat gtgacgggtc gacagccagc aatgcgagat
     2761 gcgctgtttc ctcgccgaaa tccatgatgg caaaaccaat gagctgtctg ccatcgcgtg
     2821 ccaccagcac gagggcttcc ggatcgcgga tcgcgcgcag gacgcgggct ccggtccatg
     2881 accatcccaa tccggtttca ataaggtcac gtgacatgcg cgcgatggcc agggcatcgg
     2941 cctccgccgc aagtatgaac gcgatgctgt ctgcggcgga tcgggtcatg ccaggaatat
     3001 ccaatagaac agaccgccta ttaaagcgta tttgagcgcg taataggcaa agcggcttct
     3061 ggctttcagt cgtttgccca gcaggcggat gcggtacacg taattaaaca cccggttcag
     3121 tacgccgacg ggttcgcctt cgatgttctt cgtcttgctc gctcgcacca gcgtattggc
     3181 cacgaaatcg ttgaagcgct tcatcagttt ggtcttcagc ggccgttcga catcgcaaaa
     3241 caggataagg cgggtcatat cgctcctgtt ttcggcgtag tgaatgtagg tctcatcaaa
     3301 caatacgtct tcaccgtcct tccacacgta tggctcgcca tcgatgaaaa tccggcaagt
     3361 gtcgtcgttc ggcgtgacca gccccaggtg atagcgcagc gagccagcgt acggatcgcg
     3421 gtgtgcgact aatctcccgc ctggcggcaa cagggcaaac attgcgccgt ggatgctcgg
     3481 aatcgattgc acgagttcga ccgttttcgg acacatagcc cgcgctgacg gcaacggttc
     3541 gtcgtaccac tttaaataaa agcgcttcca gccgctgcgg aagaaagaat tgaagccaac
     3601 gtcattgtag ccttcggctg cacggatata gcccgcgccg aacagcgtca gcgcttcgtc
     3661 ccggatggtc acccagttct cacgtagccg cgcaagctct ggaaagcgct ctatttccag
     3721 ccgcggccgg ttcggcacag cagagaaggc gtatagcacc gcattgagcg gggcgaaaat
     3781 ggtcgagtga tcggtgacct ggcgggtgaa cccatgccgt accctgccgc gcaggtgaac
     3841 aaaaaccgcc gccgctatga atccgtagag cagcaggaat cttggttcga atacgtgcgc
     3901 aatcattgtc acaaccctct tgcagccgcc tgagccggtg cgcgagatgc agagggcgca
     3961 cacgaggccg atcggaatgc cagcaacgat gcaagtttat ccttttcaga ggcgtcgatc
     4021 caaatccgac gattcccggc cggtggaggt cagcttcaac agatccttaa ccaggatatc
     4081 gtggcgacct ccttttactg cgaggaaaac ttcccgcaac agcatccaca gcgacccaat
     4141 caatgcaacc atggccagaa tgaacaaggt tccaatcaac cgcgcgatat caatggcgac
     4201 aaaaacgccg agaaacgcac ttgccaccag catgcaaatg aataatgcgc agacgatcgc
     4261 caatacgatt gcgtggtata tcaggctgat gcggctggcc agcagctgca attcattgac
     4321 atccgcctga tccgaccgtt cgctttgcgc aaacgcctga cgcaggcgct cttcgaggac
     4381 gcggcgccgg tcaacaattc ggccaagccg gttgttcagt gccgacagaa tagtgccgat
     4441 cgcagtcagc aggaacaccg gcgcgacggc tagctgaatg atgcgggtaa tgccttctac
     4501 gtgttccatg tcgcccgccg cctaaaggca tcagctggga tgttcacaac ggctcgaatt
     4561 ccagcgtctg catcagcatg gcgccttctt gagcaaactg cggatccaga tcttccggga
     4621 aatggcggtg tgaataggcc atgttgatgg cccgcagtgg gtcctgtggg tgcgggcaag
     4681 tcaacgtcat ggtttccagc atcgtattga cgggcgacgc ggttccgtcc gaggacgtgc
     4741 gctctgccat ttccaagcgg gacagcgcgc aggtttgctg atggactttt tgctcgctgg
     4801 tttccagctt gaacagccgg aaacgttttg gatcaatttc cttccgcagg cttgattcca
     4861 catagcgcat cattgcgtcc gaggaagtaa aggccggcag cttgatgatg gttgtctgca
     4921 tgctgaagga ttcgcccgtg aagcgtcctt gcttgccaat gacggtgagt tccgggctct
     4981 gttgggcaac tacccaggtt ttttcttttt ccttgggcgc ggaaatggaa aatccctgca
     5041 tcatgatgcg tgggggttcc ttgggttgcg atgcgcaggc gccgagggcg gcacacagcg
     5101 tccagataag ccatccagtc gatttcattc ttatctctct gctccttggt gcggcggagg
     5161 ggaaatcggg caagccacgc tagttctgaa tgagtttccc gacgggccgt gcatcgaatg
     5221 tgcagggcgc atcctgccaa gcgggattat gccgcacgtg agccggcatg gctgcatacg
     5281 gatcctgccg caggaagctt ttccttaacc cccggccgat aaagcgggcc cggactggcg
     5341 atttgctata ggattacgcc ccgggacaca agatatcggt gcccagcgcc tgacagatcg
     5401 cttcattctt cctgccttct gtattttggc gccgtgataa acgagagtgt gctgtcctgg
     5461 caacttcgtt gccgcaggac ccggcaggca ttcctggttg aagaatgttt atgcgaattt
     5521 g
//
LOCUS       DATMZQ010000113         3172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2851557, whole genome shotgun sequence.
ACCESSION   DATMZQ010000113 DATMZQ010000000
VERSION     DATMZQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2851557"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VN496_03605"
     CDS             <1..271
                     /locus_tag="VN496_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16061.1"
                     /translation="YYADPETRKVPRTLAPKTAHHLMFSFKRSGSVAIGEADDDTVTV
                     SSELLWQAQRDAVRLYGFNVTHMSILETTDSSMLINEIMIKALKD"
     gene            337..1776
                     /locus_tag="VN496_03610"
     CDS             337..1776
                     /locus_tag="VN496_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HXF16062.1"
                     /translation="MSARHRARSRDTAMARLGCALVAMAALSACQTPAPPMPVQSPPK
                     PQLSYLNIAISIPLAPVAAAVDNEVPRTAGVAPFEYWVNGGANPPACGIDAGYAVARG
                     PLVMSGSGNAIRTDMALSYWLQGRKQIPCPGDFVTASCGTDPEEARTAKVSMDTAVAI
                     LPDLTASVHSNIGPIVPGNRCVLNPAGLDITDALMAGFADGLKPVLANLDQRLAAELQ
                     LRQRVEAGWARMNEPVELRPGIWLAMNPEGIGVAPISVSNEELRTGIQLRLRPVVGAG
                     GKPEVVARPFPNADTAAAADTFEMHIPVEVEQSFVQARLDDALDLKNGGTTVSLGSYT
                     VRVTSADVYGEGSQVAIKLLFKGDVNGTAYLRGTPFYDAGSRKLSFPDLDYTLETDRA
                     LLNSANWVAQGQIRERLRTRFTVEMDRPIEAMKQSLENVLNRQRGNVNLHGNVQELHL
                     VGVYRLPNGSVFTAYLAATGKIWAEVDVQ"
     gene            complement(1841..3106)
                     /locus_tag="VN496_03615"
     CDS             complement(1841..3106)
                     /locus_tag="VN496_03615"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXF16063.1"
                     /translation="MFIIVVTFVFSSCSFVFFVPYTFTFQRKLAIIRGHPVAKRQTGL
                     CPRASESVTKIRPLRCSCSGIKIGRYRRRHPKDAQLECVRLVGQVPEPAGVVISLSDR
                     FIMIHLGIDIAKLKFDVALLAQGKFKTKVFANDSAGISACLKWLGTHADGPVHTCLEA
                     TGSYGEALACALHDAGHTVSVINPARTKAYAQSLGLRQKTDAVDAKMLARFVQTQEPA
                     VWTPPPAELRALQALLRRLDALVEMRTQEKNRLGVAEPTVSASIAQHLDYLQSQIDAL
                     KAQIENHIDQHPGLKQQQDLLDSIPGIGAATSAWLIGELNLTRFDSARQAAAFVGLTP
                     RKDDSGTSVRGRPRLSKQGNSLLRKLLYFPALTAMTHNPLVRALCLRLKAKGKHNMAI
                     IAAAMRKLIHIAFGVLKSGKPFDPTLADT"
BASE COUNT          662 a          896 c          979 g          635 t
ORIGIN      
        1 ctattacgcc gatccggaaa ccagaaaagt gccgcgcacg ctggcgccaa agaccgcgca
       61 tcatctgatg ttttcgttca agcgcagcgg cagcgttgca atcggcgagg cggacgacga
      121 taccgtcacg gtgtcgagtg agttgctatg gcaagcgcag cgcgatgcgg tgcggcttta
      181 cggttttaac gtcacgcaca tgagcattct ggagaccacc gattcgtcaa tgctcatcaa
      241 cgaaatcatg atcaaggcgc tcaaggacta gcgcgaattg cacacagtta ctcgagatgg
      301 tggctcacga gtgcggatac cggggagggt aaagacatgt cagcgaggca cagagcgcgt
      361 tctcgagata ctgcgatggc gcgtttgggc tgcgcgctgg ttgcaatggc tgcgctttcg
      421 gcttgccaga ccccggcacc gccgatgccc gtccagtcac cgccgaaacc tcaactgtct
      481 tatctgaata tcgccatcag cattccgctc gctccggtgg ccgcggcggt tgacaatgaa
      541 gttccgcgca ctgccggcgt cgcgccgttt gaatattggg taaacggcgg cgcaaatccg
      601 cccgcctgcg ggatcgatgc cggttatgcg gtcgctcgcg ggccgttggt catgtccgga
      661 tccggcaacg cgatcaggac cgacatggca ctgagctact ggctccaggg ccgcaagcaa
      721 atcccctgcc ccggtgattt cgtcacagcg tcgtgcggca cggaccccga ggaagctcgg
      781 accgcgaaag tatccatgga taccgctgtc gccatccttc ccgacctgac tgcgagcgtg
      841 cactcgaata ttggaccgat cgtgcccggc aaccgatgcg tgctgaatcc ggccggactc
      901 gacatcacag atgcactgat ggccgggttt gcggatgggc tgaaacccgt gctggcaaac
      961 ctcgatcaac gtctcgctgc agaactgcag ttgcggcaac gggtcgaggc cggctgggcc
     1021 agaatgaacg aaccggtcga attgcgtccg ggcatctggc tggcgatgaa cccggaaggc
     1081 atcggggtcg cgccgatctc ggtatcgaac gaagaattgc gcaccggaat ccagttgcgg
     1141 ttgaggccgg tggtcggtgc gggcgggaaa cccgaagtcg tggcaaggcc tttcccgaac
     1201 gccgacaccg ctgcggcagc ggatactttc gagatgcaca ttccggtgga agtcgagcag
     1261 tcgttcgtgc aggcgcgtct cgacgatgcg ctggacctga agaatggcgg cactacggtt
     1321 tctttgggca gctacaccgt tcgcgtgaca agtgcggatg tttacgggga aggatcgcaa
     1381 gtcgccatca aactgctatt caaaggcgac gtgaacggga ctgcgtatct gcgcggcacg
     1441 cctttctacg atgcgggaag ccgcaagctc agtttcccgg atctcgacta cacgctcgaa
     1501 acggaccgtg ccctgctcaa ttccgccaac tgggtcgcgc agggtcagat ccgcgagcgc
     1561 ctgcgtaccc ggttcactgt cgagatggac cggccgatcg aggcaatgaa gcagagcctg
     1621 gagaacgtgc ttaatcgcca aaggggcaac gttaatctgc acggcaacgt acaggagctg
     1681 catttagtgg gcgtctacag gctgccgaac ggcagcgtat tcactgcata cctggctgca
     1741 actggaaaga tctgggccga agtcgatgtg cagtgatgcc ggctctaatg ccataagcgt
     1801 atcctgccta catctcacca cgaagatacc gtgttgcatg tcaagtatca gcaagtgtcg
     1861 gatcaaaggg tttgccggac ttgagtacgc cgaacgcgat atgaatgagc tttcgcatgg
     1921 ccgcagcaat gatggccatg ttgtgtttgc ctttggcctt gagtctcagg cacaaggcgc
     1981 gcaccaatgg gttatgagtc attgcagtca gcgccgggaa gtagagcaac ttgcgcaaca
     2041 gactgttgcc ttgcttggag agtcgcggtc tgccacgcac ggaagtgccc gagtcatcct
     2101 tgcgtggggt gagtccgaca aaggcggcgg cctggcgggc gctgtcaaag cgcgtgaggt
     2161 tgagctcccc gatgagccag gcgctggtgg cagcgccgat gccggggatg gaatcgagca
     2221 gatcctgttg ctgcttcagt cccggatgct gatcgatatg gttttcgatc tgggctttga
     2281 gcgcatcgat ttgggactgg aggtagtcga gatgttgcgc gatggaggca gacacagtgg
     2341 gctcggctac acccaggcgg tttttttcct gggtgcgcat ctcgacgagc gcgtcaagcc
     2401 ggcgcaacag ggcctgcagt gcgcgaagct ccgcgggcgg cggcgtccaa acggcgggct
     2461 cttgggtctg gacgaagcgc gcgagcatct tcgcatccac cgcatcggtc ttctggcgca
     2521 gccccaggct ctgggcgtag gccttggtgc gcgccgggtt gatgacactc accgtatgcc
     2581 ccgcatcatg cagcgcgcag gcgagggctt cgccgtaaga gccggtggct tccaggcacg
     2641 tgtgcaccgg gccatcggcg tgggtgccca gccacttcag gcaggcactg atgcctgctg
     2701 aatcgttggc gaagactttg gtcttgaact ttccctgcgc gagcagggcc acatcgaact
     2761 tgagtttggc aatatcgatt ccaagatgga tcatgatgaa cctgtcagat aatgaaatga
     2821 cgactccagc gggctcaggc acttgtccaa ccaaccttac acactcaagc tgtgcatcct
     2881 ttggatgccg ccttcgatac cgtccgattt ttatgcctga acaagaacag cgaagcggcc
     2941 tgatcttcgt gacagactct gaagctcttg gacacaaacc agtttgccgc ttcgccaccg
     3001 ggtgcccccg gattattgcc agctttcgct gaaacgtaaa cgtataaggc acgaagaaca
     3061 cgaaggagca cgaagaaaaa acgaacgtca ctactataat gaacatggac cgcaatttgt
     3121 catgaacgat tgggtaacgt ccagaatcaa cctgtctttg cccttcttcg tg
//
LOCUS       DATMZQ010000114         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4878653, whole genome shotgun sequence.
ACCESSION   DATMZQ010000114 DATMZQ010000000
VERSION     DATMZQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4878653"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VN496_03620"
     CDS             complement(<1..844)
                     /locus_tag="VN496_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF16064.1"
                     /translation="MPPALLALAAAAFVSGANLRLFDALLPTVAEDFGVPPTTASVVV
                     TAFTLAYGLFQIVHGPLGDRIGKLRVIGGATLIAAAASLGCAFAPTLSALAALRFATG
                     IGAAAIVPLALAWIGDNTPYDKRQATLGRFLSFILMGQILGPALGGALAEFMNWRRVF
                     DAMALIFLAVSIVLFGVDRHTRVATVAKTHGNVWRNYAAVLGDRWVRIIIIAVFLEGG
                     LFYGVFAYTGAYLKERFALSYLLIGAMLAGFGLGGMIYSLLVKWLLTRLGEQGFVRLG
                     ATLMF"
     gene            950..1714
                     /locus_tag="VN496_03625"
     CDS             950..1714
                     /locus_tag="VN496_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HXF16065.1"
                     /translation="MNTPLNPRNARVGGRWVITGLVLSLASAAAMVFSGIGYRLDLFH
                     FRVGFTILRWAFWFALAGGLLSLAGLIRTRGRPGSMLVAAIIGLAAGMTAAYIPWSLK
                     RTAESLPYIHDISTDTANPPEFVAAAKLRKPGDHPVTYDGKEVADLQHQAYPDIAAFT
                     SSASSEKAFEAAKAAIASMGMQLVDADPAQGRIEATQTSLLYGFTDDMVVRISKAAEG
                     TKVDVRSKSRVGRSDLGQNAKRIRTFVQKMKTSLAS"
     gene            complement(1707..2102)
                     /locus_tag="VN496_03630"
     CDS             complement(1707..2102)
                     /locus_tag="VN496_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16066.1"
                     /translation="MADIEATDAVANSSITLAQVIYGLHALSILIGVTSAVTIVGAFV
                     FGVPSIIAVIINYAYRHTVRGTFLESHFNWQIRTFWFAFLWFVVGVMLFVTLVGIPLA
                     WIVCIAAGAWVIYRVARGWLTLQDRKPIS"
     gene            complement(2170..>2971)
                     /locus_tag="VN496_03635"
     CDS             complement(2170..>2971)
                     /locus_tag="VN496_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXF16067.1"
                     /translation="LAYVAVAFSVHIDWARAIQQSVAPALSFNPDFIAVVVAVFGTTI
                     SPYLFFWQASQEVEELRGGNGSSLKSTPIDARAHLRRIKIDTYIGMGFSNLIAFFIIL
                     SAAVTLHVSGITAIQTSAQAAQALRPIAGEFSFLLFSLGIIGTGMLAVPVLAGATAYA
                     VADSFGWRQGLDRKLFEATEFYTIIALATLGGVLLDFTPIDPIKALFWSAVINGVIAV
                     PIMVVMMLMATRSEVMGPFVIRRRLKILGWLATVAMALAVISMVITAR"
BASE COUNT          695 a          892 c          878 g          506 t
ORIGIN      
        1 caaacatcag cgtagcaccc agtcgcacaa aaccttgctc gccaagccgt gtcagcagcc
       61 atttgacgag caggctgtag atcatgccgc cgagcccgaa ccccgccagc attgcgccga
      121 tcaataaata ggaaagagcg aagcgctcct tgaggtaagc gccagtgtag gcaaacacgc
      181 cgtaaaacag gccaccttcg aggaataccg cgataatgat gatgcgcacc cagcgatctc
      241 cgagcacggc ggcatagttg cgccagacgt tgccgtgggt tttcgcgacc gtggcaacgc
      301 gtgtgtggcg atcgacgccg aacagcacga tgctgaccgc caaaaatatc aacgccatcg
      361 catcgaacac ccgacgccag ttcatgaact cggccaatgc gccgcccagc gcgggcccaa
      421 ggatctggcc catcaggatg aaacttaaaa atcggccgag cgtggcctgg cgcttgtcat
      481 atggcgtgtt gtcgccgatc caagccagcg caagcggcac gatggcggca gcgccaatgc
      541 cggtcgcgaa gcgcaacgcc gccagcgcgg aaagtgttgg cgcgaatgcg caacccagcg
      601 aagccgccgc ggcgatcagc gtggcgccgc cgattacgcg cagcttgcca atgcggtctc
      661 cgagagggcc gtgcacgatc tggaataacc cataggcgag cgtgaatgca gtcacaacca
      721 cggacgcggt cgtcggcggt accccgaaat cttctgctac cgtaggcaat agcgcatcga
      781 acaggcgcag attcgcgccg gacacgaacg cggcagcagc aagggcaagc agggctggag
      841 gcatggggta ggccgcattc tgcccggttg gaagtcttaa atcgacaaaa actcggcgta
      901 gaatcttgcg cgacgccgat ggtggatcac gagacacaaa ggagcagaca tgaacacacc
      961 gctaaatccc cgcaacgccc gcgtgggcgg gcgctgggtc atcaccgggt tggtgctttc
     1021 cctcgcaagc gccgctgcga tggtgttctc gggaatcgga taccggctgg atctttttca
     1081 tttccgtgtc ggctttacga ttctgcgctg ggcattctgg tttgcgctgg ccggcggact
     1141 gctttccctg gcaggtttga tccgtacgcg cggccgccca ggcagcatgt tggtcgccgc
     1201 catcataggg ctcgcggcgg gcatgaccgc tgcatacatt ccatggagtc tcaagcggac
     1261 tgccgagtca ctgccttaca tccacgacat cagtaccgat accgccaatc cgccagaatt
     1321 cgtcgccgcc gcaaaactgc gcaagccggg cgatcatcca gtaacttatg acggcaaaga
     1381 agtggccgac ctgcagcacc aagcgtatcc cgatattgcg gcctttacga gttcagcgtc
     1441 cagcgagaaa gctttcgaag cggcaaaggc ggcgatcgcg tccatgggca tgcagttggt
     1501 cgacgccgac ccggcgcaag gccgcatcga agccactcag accagcctgc tctacggatt
     1561 caccgacgac atggtcgtcc gcatcagcaa ggcggcagag ggcacaaaag tcgatgtgcg
     1621 gtcgaagtcg cgcgtcggcc gcagcgatct gggacagaac gccaagcgca tccgcacgtt
     1681 cgtgcaaaaa atgaaaacga gtctggctag ctgatcggct tgcgatcctg cagtgtcagc
     1741 cagccgcggg caacgcgata gataacccac gcgccggcgg cgatgcatac gatccatgcc
     1801 agagggatac caacaagcgt cacgaacagc atcacgccaa ccacgaacca caggaaagca
     1861 aaccagaacg tgcggatctg ccaattgaag tgcgactcca gaaaagtccc gcgcacggtg
     1921 tgccggtaag cataattgat gattacggca atgatggacg gaacgccgaa tacaaaggcg
     1981 ccgacgatcg tgaccgcgct ggttacgccg atcaggatgc ttaatgcatg aagcccgtag
     2041 atgacctgcg cgagggtaat cgacgaattt gcaaccgcat ctgtggcttc gatgtctgcc
     2101 atgccgttct ccggactcga taagcaattc gacctgattg cgcccattaa attccgtcgc
     2161 tggtttgttt caccgcgctg taatcaccat ggatattact gccagggcca ttgcaaccgt
     2221 tgccagccag ccgagtatct tcaaacggcg ccggattacg aacgggccca tgacctccga
     2281 gcgcgttgcc atgagcatca tcaccaccat gatcggcacg gcgattacgc cgttaatgac
     2341 ggcgctccag aataaggcct tgattggatc gatgggtgtg aaatccagca gcacgccacc
     2401 cagagtcgcg agcgcgatga tggtgtagaa ctcggtggcc tcgaacagct tgcgatcgag
     2461 cccctggcgc cagccaaaac tgtccgcaac cgcgtaggcc gttgcgccgg ccaacacggg
     2521 aacggccagc atgccggtgc caataatgcc cagactgaac aagaggaatg aaaattcgcc
     2581 ggcaatcgga cgcagtgcct gcgctgcctg ggcggatgtc tggattgccg tgatgccgct
     2641 gacatgcaag gtcaccgccg cggacagaat aataaagaaa gcgatcaggt tggaaaatcc
     2701 catgccgatg taggtatcga tcttgatgcg acgcagatgg gctcttgcat caatcggggt
     2761 gcttttcagc gacgaaccgt tgccgccgcg cagctcttcg acttcctgcg atgcttgcca
     2821 gaagaacagg taggggctga tggtggtgcc gaacaccgct accaccacgg cgatgaaatc
     2881 gggattgaag gagagtgccg gcgcgaccga ctgctggatg gcacgcgccc agtcgatatg
     2941 caccgagaac gccaccgcca cgtaagccag c
//
LOCUS       DATMZQ010000115         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2506108, whole genome shotgun sequence.
ACCESSION   DATMZQ010000115 DATMZQ010000000
VERSION     DATMZQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506108"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VN496_03640"
     CDS             <1..521
                     /locus_tag="VN496_03640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF16068.1"
                     /translation="QLVEALAALRRQGVAARALLIGDGEMRPAVEARARDLGVAHALV
                     ITGFQNDVRPFIAAADVLVLCSTAVETFSLAALEAMALGRPVVLSDIGGAAEMIAPGG
                     NGFLFRAGDTDALTRKLSVLVDRALCERMGRSAREKVETRFSEHEMIDRYEQILQELC
                     GKQVQRNTPITT"
     gene            888..2252
                     /locus_tag="VN496_03645"
     CDS             888..2252
                     /locus_tag="VN496_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HXF16069.1"
                     /translation="MLSSFGYRRSPVWAQESLIAAKSSLRNVMREGRAFRAISVQVDK
                     SQWWSEQELREFQARRLRVIVEAAATGIPYYRDRYRKLELDFTAQAFPGVLSRLPYVT
                     KDDVRAYGRAFIPERSRGPLFSSSTSGTTGKPLQLYQDLTSINRENAFVWRQLAWAGL
                     RRGERRAWIRGDMIVPAEQRAEPYWRSNRAENMLMLSSYHLSESTARGYVEALAAFDP
                     VLIQAYPSSIGFLGSWMLSAGVRYGGSALRGIVTSSETLSEEQRRNIAQAFGCRVFDW
                     YGQGERVAAIGTCEHGRYHLLTDYSYVELLPARDGWCEVVGTGFNNIAMPLIRYRTGD
                     FVRPAPEGQRCICARPLPLIESVAGRTDDVLKLPDGRVIGRLDHIFKDVGGIIEAQIR
                     QDRSDEVTILVVPAATFSERDKESLRRNARERLGEIVSLEIRLMNAIPRTANGKLRGV
                     VCNV"
     gene            2269..>3351
                     /gene="asnB"
                     /locus_tag="VN496_03650"
     CDS             2269..>3351
                     /gene="asnB"
                     /locus_tag="VN496_03650"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXF16070.1"
                     /translation="MCGIAGYVRLRPQHCAPISAGAAGEMLAAIRRRGPDGEGEWRSP
                     DGLCWLGHRRLAIIDLRTGAQPMSNEDGKIWTVFNGEIYNHLALRAELQAHGHCFRTG
                     SDTEVLVHGYEQWGLRGLAQRLQGIFAFALYDSVKQKLSLVRDPMGVKPLYWWSDGNA
                     LLFASEMKALLRHPALRQRKVNHAGVAQVLVTRYVSRPHTMFQSVSRLPEGCCVEFAV
                     NCALPEKPARYWDVSFQPEQISVETATEQLDALLKRTVEMQLMSDVPLGVQLSGGVDS
                     SIIVALMENIRRERGDPAPVKTFSVGFDIPEFSELRHARKVARRYGTEHHELTVGFRD
                     FAEELPWLAWIYDEPMGEPPGIPTYFM"
BASE COUNT          678 a          945 c         1075 g          653 t
ORIGIN      
        1 ttcagctggt ggaggcgctt gccgcattgc gcaggcaagg cgtcgcggcc cgcgcgctgc
       61 ttatcggcga cggggaaatg cgtcccgcgg tcgaggcgcg tgcgcgtgac cttggcgtgg
      121 cccatgcgct cgtgatcacc ggctttcaaa atgatgtgcg tccattcatc gccgccgctg
      181 atgtgctggt cctgtgcagc accgcagtgg aaacattctc gctcgcggca ctcgaggcca
      241 tggcactcgg cagaccggtg gtgctgtccg acatcggtgg tgcggccgag atgattgcac
      301 cggggggcaa cggctttctt tttcgcgcag gggataccga cgcgctcacc cggaagctat
      361 ccgttctggt cgatcgcgcg ctctgcgagc gcatgggcag aagtgcgcgc gagaaagtgg
      421 aaacccggtt ttcggagcat gaaatgatcg accgttacga gcaaatattg caggagcttt
      481 gcggcaagca ggtgcagcgg aacacgccga ttacgacctg acgatccatc cggcccatgg
      541 cgaccacacg aattctaaac agcattgctg caatatgccc ttgagaaccg caggacgccc
      601 caccggcatg gaagcggtgt acggcaatcg aattggcgac ttgcaccgcg cgatattcgg
      661 tcgcccctta agctgaatca gagagtcact ggggcacaga atgttccgga aaagccgaga
      721 agagtttgct caacaagacc tccagaagca taaacggcgt ttcatcaacc cgatattcag
      781 cggcgacgag gatagcaatg ccgcgtgcat ggcagcagag acggtacgcg gggtggcagg
      841 aatcaacata ttgcacggac tccgcgcgcg cccggcatga tcgctagatg ctaagtagtt
      901 tcggatatcg ccgcagcccg gtctgggccc aagagtcgct gatcgctgcc aaatcgtcat
      961 tgcgtaacgt gatgcgagaa ggccgcgcat tcagggccat cagcgttcag gtcgataaga
     1021 gccaatggtg gtccgagcaa gagctgcgtg aatttcaggc ccgcaggctg cgagtgattg
     1081 tggaagcggc ggcgacaggc attccttatt atcgggaccg ctatcgcaag ctggagttgg
     1141 atttcacagc gcaagctttt cccggagtgt tgagccgctt gccctacgtg accaaggacg
     1201 atgtgcgtgc gtacggccgt gcattcatcc cggagcgcag ccggggcccg ctgttttcca
     1261 gcagcactag cggtaccacg ggaaagccgc tgcaactgta ccaagattta accagcatca
     1321 accgggaaaa cgctttcgtc tggcgacaac tcgcctgggc cggactccgc cggggcgagc
     1381 ggcgggcatg gatacggggc gacatgatcg tccctgcaga gcagcgcgca gaaccttatt
     1441 ggcgctccaa ccgcgccgag aacatgctta tgctttcgtc ttaccacttg tcggaatcta
     1501 ccgcgagagg ctacgtagaa gcattggccg cattcgatcc ggtgctgatt caggcctatc
     1561 cctcttctat cggattcctc ggcagctgga tgctgagcgc gggcgtacgc tatggcggaa
     1621 gcgcgctgcg cggcattgtc acttcgtcgg aaacgttgag tgaagagcag cgccggaata
     1681 tcgctcaggc cttcggctgt cgggtattcg actggtacgg acagggcgaa cgcgtcgcag
     1741 ctatcggcac ttgtgagcac ggtcgttacc acctgctgac cgactactcc tacgtcgagc
     1801 tgctgcctgc gcgggacggc tggtgtgaag tggtggggac tggattcaac aacatcgcga
     1861 tgccgcttat ccgctatcgc accggagact tcgtgcgtcc cgcgccggag ggccagcgct
     1921 gcatctgcgc ccggccgtta ccgctgattg agagtgtcgc tggtcgcacc gacgatgtgc
     1981 tcaagctgcc ggacgggcgt gtaatcgggc gcctcgatca catcttcaag gatgtcggcg
     2041 gcatcattga agcgcagatc agacaagatc gcagtgacga ggtgaccatt ctggttgttc
     2101 ctgcggcaac attcagcgag cgcgacaaag agagccttcg ccgcaatgct cgggaacgtc
     2161 tgggcgagat cgtgagcctc gaaattcggc tgatgaacgc gatcccgcgc acagcaaacg
     2221 gcaagctcag gggtgtggta tgcaacgtat aagagaggcg ggtgcgtcgt gtgtggcata
     2281 gcgggttatg tgaggctgcg accgcagcac tgtgcgccga tctccgccgg tgcggcgggt
     2341 gagatgttgg cggcgatccg gcgccggggg ccggacggag agggcgagtg gcgctcaccc
     2401 gacggtctgt gctggcttgg ccaccggcgc ctcgccatca tcgatctgcg gaccggggct
     2461 cagcccatga gtaatgagga tgggaaaatc tggactgtct tcaacggcga aatctacaat
     2521 catcttgccc tgcgggcgga gttacaggcg cacggtcatt gtttcagaac tggcagcgat
     2581 accgaggtcc tggtccacgg ttacgagcaa tgggggttgc gcgggctggc gcaacgcctg
     2641 cagggaatct tcgcttttgc actctatgac tcagtcaagc agaagttgtc gctagtgcgt
     2701 gacccgatgg gagtcaagcc gctctactgg tggagcgacg gcaatgcatt gctgtttgcc
     2761 agcgagatga aggcgctgtt gcgtcatcca gccctgcgcc aacgcaaggt taatcatgcg
     2821 ggtgtcgccc aggttctcgt cacccgctat gtatcccgcc cgcacaccat gttccaaagc
     2881 gtgtcgcgcc tgccggaagg atgctgcgtc gagtttgccg ttaactgcgc actgccggaa
     2941 aagccagcgc gctactggga tgtcagtttc cagccggagc agataagcgt cgagacagca
     3001 accgagcaac tggatgcact acttaagcga acggtggaaa tgcagttgat gtcagacgtg
     3061 ccactggggg tgcaactctc cgggggagtc gacagcagca tcatcgtcgc cttgatggag
     3121 aatatacgcc gggaacgcgg agatccggcg ccagtaaaaa ccttttccgt cggtttcgat
     3181 atcccggaat tcagcgagct ccggcacgca cgcaaggtgg cgcggcgtta cggcaccgag
     3241 catcacgagc tcaccgttgg tttcagggat tttgcagagg aattgccctg gctggcctgg
     3301 atatacgacg aaccgatggg ggagcctccg ggaataccga cctatttcat g
//
LOCUS       DATMZQ010000116        11588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4879349, whole genome shotgun sequence.
ACCESSION   DATMZQ010000116 DATMZQ010000000
VERSION     DATMZQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4879349"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VN496_03655"
     CDS             complement(<1..490)
                     /locus_tag="VN496_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16071.1"
                     /translation="MSDLQIGLALLGALIVIAVLAFNYWQERQFQRRGAQSFTGHHED
                     VLLDQPATQPAASSGPALAADDNRIEPRMEPELEEPAAVIASQAQETSVDMPRREIDY
                     IAELRAGEFIGAHHIAKVRHRIPALGRRITLGGLNHESKSWEPLRTDGRYTTLRLGLQ
                     LVD"
     gene            complement(530..3613)
                     /gene="smc"
                     /locus_tag="VN496_03660"
     CDS             complement(530..3613)
                     /gene="smc"
                     /locus_tag="VN496_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HXF16072.1"
                     /translation="MISRIIEAKPEDLRSFLEEAAGVSKYRERRRETELRLQDTRENL
                     LRVDDIRQELGKQLEHLELQAEKAGKYRGFEASLKRTQQLLWTLRKRDAQAQRIKISR
                     DVEAMGIELEAETARLRDAERRVEELRTGHYGASDAVHAAQGTLYEANAEIARLEQQI
                     QFHRDSRHRAEQQLNALRAQDAEQKERQQALQSGRAEAVRALAEASEQLASLENALHE
                     ARIALPAAEENYRMAQALHAQAQTALTQAEQQLQLEDTRKGHSDKLLQQLDLRRARLE
                     EERDGLEIPNLSELVRLESEIRELAHELDTRRLAFEQSEVALPTHEEVLQAAENVAER
                     LQQRVHQVDARLTALQSLQDQVSRSSALEPWLDRHGLRRASHLWQGIHIRDGWDDALE
                     AVLRERLNSIGLTDLGQAHGWLAEPPPGKLSFHRTGELPEFKDQPPPGLEPLTDHVTC
                     TDPRLAPVLADWLNGVYVLADPAQGIEKAAMLPPGAMLVSPQGHVFTATSISFHAADS
                     EIHGILARQREIEALFRERTDADARLVELNHDAASRKIEFEERRGQVETLRREVSELQ
                     ERHHQLNLDHVRLSQLAERLKARGEQIVAELAEIDAESARESAQRGQAALEMEGLHAG
                     MAAMHGAVDQARQRYEQAETDLESSRALAQQIERAEQEALFQNKTIGSKIIDIDSALE
                     LIAQTLSRLSADVENLLQELSASNESVLQEQLQQQLAVRAGREHSLKQAREELDTAEQ
                     TLRATEQERLASEQKLAPARDRINELRLKEQEARLAEESFASQLAEVQANEEELLGLI
                     EEGMRASKLQTEINRLNEELAALGAVNLAALEELSGARERKSYLDSQSLDLTEAVDTL
                     ETAIRKIDRETRERLQDTFDAVNKHFGEMFPALFGGGEAKLLMTGEEILDAGVQVIAR
                     PPGKRNASIHLLSGGEKALTALSLVFSMFQLNPAPFCLLDEVDAPLDDSNTDRFCSLV
                     KKMSTNTQFMFITHNKITMEMASQLIGVTMQEQGVSRVVAVDVDEALRMREEAAA"
     gene            complement(3610..4053)
                     /locus_tag="VN496_03665"
     CDS             complement(3610..4053)
                     /locus_tag="VN496_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF16073.1"
                     /translation="MRLTHIKLAGFKSFVDPTHIPVPGQLVGVVGPNGCGKSNVIDAI
                     RWVLGESSAKQLRGEHMQDVIFNGSGERKPVGRASVELIFDNSLGKAAGPWSAYAEIS
                     VKRVLERDGESSYFLNNLHVRRRDIADIFLGTGLGGRGYASSNRA"
     gene            4182..4598
                     /gene="queF"
                     /locus_tag="VN496_03670"
     CDS             4182..4598
                     /gene="queF"
                     /locus_tag="VN496_03670"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXF16074.1"
                     /translation="MPSTPSLSLDTFPNPNPARDYLIHMEIPEFTCLCPKTGQPDFAT
                     LLLDYVPDKKCVELKSLKLYVWSFRDQGAFHEAVTNRMLDDLAEATQPRFMRLTARFY
                     VRGGIFTTVSAEHVKNGWKPKPRIDVAQFESDSPVR"
     gene            complement(4584..5471)
                     /locus_tag="VN496_03675"
     CDS             complement(4584..5471)
                     /locus_tag="VN496_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF16075.1"
                     /translation="MTAQKNNMVAVASLLTGALVWGVIWYPYRALQSAGLGGASASLV
                     TYLLPLLAGSLLFRKRGVQLRKAPWLLVAIAISAGLCNVGYVLAMLYGEVVRVMLLFY
                     LAPLWTLVFAWLLLGEVAGPRGYPVIALSIAGAVVMLWQPGGRWPLPYNSAEWLGLGS
                     GMAFALSNVLSRKAVHIDETIKAGAVWAGVSAVALVVALLTEQPVSAFASVDAAAWLL
                     VGLVALVLFCVNLVVQFGLAHTSANRAIVIMLSELVFAAASSYLLAFEHINWRDLVGG
                     AMLVAAALYSGRLEPSTHR"
     gene            5507..6778
                     /gene="dapC"
                     /locus_tag="VN496_03680"
     CDS             5507..6778
                     /gene="dapC"
                     /locus_tag="VN496_03680"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HXF16076.1"
                     /translation="MQSAEMNANLSRLQPYPFQKLAALLAGITPDAALRPISLSIGEP
                     KHPTPQFIRDALIQQLGGLSSYPATAGSEALRSAIARWIEQRYRIDSIDAAKQVLPVN
                     GSREALFAFAQAVVDTSDNTPAVVAPNPFYQIYEGAIILAGAEPHFLNLLPENGFALN
                     LGQIAQETWKRTQLVYVCTPGNPTGNVMNMEEWSQLFGLSDRYGFVIASDECYSEIYF
                     DDNRPPLGGLEAAYRLGRKDFRRLVAFSSLSKRSNVPGMRSGFVAGDAEILAKFLLYR
                     TYHGCAMNPAVQAASATAWSDEQHVAENRRLYREKFDEARAILSPVTEVNMPDAAFYL
                     WLRTPIADTEFARQLFEQFNVTVVPGSYLAREAHGVNPGRNFIRVALVAAVEECVEAL
                     QRLAAFYGQFSAGKDSNDAVASLNTRRQATP"
     gene            complement(6868..7173)
                     /locus_tag="VN496_03685"
     CDS             complement(6868..7173)
                     /locus_tag="VN496_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16077.1"
                     /translation="MKMLMIVFRESIEEEIKGLLKEHGVRAFTEMHEVTGVGEAGAAF
                     HSLSWPAYNDMILTALPDSKAETVVAGLRSFRDGAMQKQHGGKIPLRVFSLPCELLV"
     gene            complement(7358..7885)
                     /locus_tag="VN496_03690"
     CDS             complement(7358..7885)
                     /locus_tag="VN496_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16078.1"
                     /translation="MKNMYLTLATLVVLFAASSTWAQQKYSITEGPKSESKYLQEQII
                     DVGDRPGHQLRIYELRNTYPANDLEFAGVKVTESHVRGTSDYTNWSGSFVTYTVYSLE
                     DGSQIFSRGTGTTQSKMGGDGKRQSFKYSFVETFIGGTKGFRGIRGQQTGSGAREAGA
                     NSLSNLQSVGEYWIE"
     gene            8267..8893
                     /locus_tag="VN496_03695"
     CDS             8267..8893
                     /locus_tag="VN496_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXF16079.1"
                     /translation="MPDATFDERYLNSLAATGTVRAYAKNTLLIQEGDFSDQLYVVLA
                     GRLKVFLSDSDGKEVIIDTLRQRDCFGEMALEGEPRSASVMTLEPCKLAVIERDQFKQ
                     FLSLYPEAAYALIVTVIRRARNLTRAIGSLALLDVYGRVARLLLDNASEENGKLVVGE
                     RMTQQDISKRISSSREMVSRILTDLREGGYIGIEEDRIVILRALPARW"
     gene            complement(8925..11459)
                     /locus_tag="VN496_03700"
     CDS             complement(8925..11459)
                     /locus_tag="VN496_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF16080.1"
                     /translation="MVGREQEIALLRDRWKLSRTGQGQVVLVQGEPGIGKSRMLTAFR
                     QQLGENIEIALHYQCSPYHINSALYPIANHLERALDFQRDDTAAQKVNKIEQRLILEL
                     GHSRTDCHLLALALSITGDDRYGVLDMSPQRRKEEIIALLVNIVAAIARKQATVLLFE
                     DAHWADPSTLEVLNALIDSANKIPLLVLITYRPEFAPAWMDVPHVTRIGLRRLSRVQG
                     AGIVLRVAEDKPLPAELVSHIVDKTDGVPLFLEELTKSVLESDLLIEVDERYEYSGTH
                     SELAIPATLRDSLAARLDRLIPLKEIAQVGAVLGREFSYELARAVSRMSEAQFHEALD
                     KLTESELVSRRGTPPEATYTFKHALVRDAAYDSLLKTQRQLLHGQIAEAISERFPSRV
                     RTEPELLAQHYTEAGMLETAIPLWQKAGELAQKRVALQEAIGHFERGLEVTSRLAPSR
                     QRDTWELQLRALLGMAWVELHGYTHPQVAANLEPALALDQSLDDGDYTLRVLWGLWVY
                     TLCAGRVEDSLSWAKRLIDVAEDRDSESMRLAGHWAACDSHYFLGNFSRSIYHADIIL
                     ARYNPERDRGVADLINHDPKTIALAYRAAAEWRLGFPDRAAASAKLAVANSRERKHVF
                     DFCWVHTFLAHTLLTDSSDTMLMDEFLNEIERFALDQKLVFFTQVYCPLSRAFWLLRC
                     HRAKEADAKFRQVIPEWQAAGMGIYMPTINAMHAETLRFCGDPGTALLLLEKALEQIN
                     KTGCQERGALSNVLRLKACIFGEMGNVEKAEVIFKEALLVARQQSAKSWELRAAISYA
                     RVLRDQSRCKEALNLIRPVYDWFTEGHDSGDLQEAAALIAKLDAVV"
BASE COUNT         2368 a         3471 c         3194 g         2555 t
ORIGIN      
        1 ggtccacgag ctgaaggccc agccgcaagg tggtatagcg cccgtcagtc ctcaacggtt
       61 cccacgattt cgattcgtga ttaagcccgc ccaaagtgat cctccggcca agtgccggaa
      121 tccgatgccg gacttttgca atatggtggg ctccaatgaa ctcgcctgcc ctgagttcag
      181 cgatgtaatc gatttcccga cgtggcatgt cgacgcttgt ttcctgcgcc tgggacgcaa
      241 tcactgcagc cggctcctca agctccggct ccatcctggg ttcaatgcga ttgtcatcgg
      301 ctgcaagagc cggccccgag gatgccgcgg gctgcgtagc aggctggtcc agcaacacgt
      361 cttcgtgatg accggtgaag ctttgcgcgc cacgtcgctg gaattgccgc tcctgccagt
      421 agttgaacgc caatacggca ataacgatga gggcgcccag tagggcaagg ccgatctgca
      481 agtcactcat ggccctgctc tcgacccgcc gccgtgtgca ggagcaatat caagctgccg
      541 cctcttcgcg catgcgcagc gcctcatcaa catccacagc aacgacccgc gatacaccct
      601 gctcctgcat ggtgacaccg atcaactggc ttgccatttc catggtgatc ttgttgtggg
      661 tgatgaacat gaactgcgtg ttggtcgaca ttttcttcac caggctgcaa aagcggtccg
      721 tgttgctgtc gtcgagcggg gcgtcgacct cgtccaacag gcagaagggg gccggattaa
      781 gctggaacat ggagaatacc agcgacaagg ccgtcaatgc tttttccccg ccggacaaca
      841 gatgaatgga cgcattgcgt tttcctggcg gacgcgcgat aacctgcacc ccggcatcga
      901 ggatttcctc tccagtcatt agcaattttg cttcgccacc gccgaacaag gccgggaaca
      961 tctctccaaa atgtttgttg acggcatcga aggtgtcctg cagacgttcg cgggtctcgc
     1021 ggtcgatctt gcgtatcgcc gtttccaggg tatcgaccgc ttccgtaaga tccagggatt
     1081 gggagtccag gtagctcttg cgctcccgcg cccccgacaa ttcttccagc gcggccaggt
     1141 ttactgcacc gagcgctgcg agctcttcgt tgagccgatt gatttccgtc tgcagtttgc
     1201 tcgcacgcat gccctcctcg atcaatccca gtagttcttc ttcgttggcc tgaacttcgg
     1261 cgagttggct ggcaaaactt tcctcggcaa gccgcgcttc ctgctccttg agacgaagct
     1321 cattgatacg atcccgcgcg ggtgcgagtt tttgctcgga tgccaggcgt tcctgttcgg
     1381 ttgcccgcaa tgtctgctcc gcggtgtcga gttcttcgcg cgcctgcttc agggaatgct
     1441 cccgtcccgc ccgcaccgcc agttgttgct gcaactgctc ctgcaacacg gactcgttgc
     1501 ttgccgacag ctcctgcagc aggttttcca catccgcact gagccgcgac agagtctgag
     1561 caattaactc aagagcagaa tctatatcaa tgattttgct gccaatagtc ttgttctgaa
     1621 agagcgcctc ctgctcggcg cgttcaattt gctgggccaa ggcacgactg gactccaaat
     1681 cggtttcggc ctgctcgtag cgctgcctcg cctgatcaac ggcgccatgc atggctgcca
     1741 tgccggcgtg cagcccttcc atctccaggg cggcttgccc ccgttgcgcg gattcgcgtg
     1801 cagattccgc gtcgatttcg gccagttcgg caacaatctg ctcgccgcgc gctttcaggc
     1861 gttccgccaa ctgcgagagc cgtacatggt ccagattaag ttgatggtgc ctttcctgta
     1921 actccgagac ctcccgtcgc agagtttcca cttgtcctcg acgttcttcg aattcgattt
     1981 tccgggaagc agcatcgtgg ttcagctcga ccaaccgggc atcggcatcc gtgcgttctc
     2041 tgaacagcgc ctcgatttcc cgctgacgcg ccagaatgcc gtgaatctct gaatcagcgg
     2101 cgtggaaact gatgctggtc gccgtaaaga catgaccttg cgggctgact aacatcgcgc
     2161 cggggggaag cattgctgct ttttcgattc cttgcgccgg atccgccaat acataaaccc
     2221 cattcagcca gtcggccagc accggcgcca aacgcggatc ggtgcaagtg acatggtcgg
     2281 tcagaggttc caggccgggt ggcggctgat ccttgaattc gggaagttcg ccagtacgat
     2341 gaaatgaaag tttgcccggc gggggctcgg ccagccaacc atgtgcctgt cccaggtcgg
     2401 tcagtccgat gctgttcagg cgttcgcgca aaactgcctc cagagcatcg tcccacccat
     2461 cccgaatatg gatgccttgc cataaatgcg acgcgcggcg caagccgtgg cgatcaagcc
     2521 aaggctcgag agcgctggaa cggctcactt gatcctgcaa cgactgcagg gcggtcagac
     2581 gtgcatcgac ttgatgcacc cgttgttgaa gccgttcggc aacattttcc gcagcctgca
     2641 gcacttcctc gtgagtgggc agcgccacct cggactgttc gaatgccagg cgccgagtgt
     2701 ccagctcgtg ggcgagttct cgtatctcgg actcgagcct gaccagctcg ctcaggttcg
     2761 ggatctccag accgtcgcgc tcttcctcca agcgggcgcg gcgcaaatcg agttgctgca
     2821 ggagtttgtc actgtggccc ttgcgtgtat cttccagctg caattgctgc tcggcctgcg
     2881 tgagcgccgt ttgcgcctga gcatgcagcg cctgtgccat acggtaattt tcttccgcgg
     2941 ctggcagtgc tatgcgcgct tcatgcaacg cattttccag tgaagcgagc tgctccgaag
     3001 cctcggccag cgcacgcacg gcctcggcgc gcccgctttg cagcgcctgc tggcgctctt
     3061 tctgctcagc gtcctgcgcg cgcagcgcat tcagctgctg ttcggcgcga tggcggctgt
     3121 cgcgatgaaa ctgaatctgc tgctccaggc gcgcgatttc cgcgtttgct tcatacagcg
     3181 ttccctgcgc tgcatgcacg gcgtcggatg cgccgtagtg cccggtgcgc agttcttcga
     3241 cccgcctttc cgcgtcgcgc agccgtgcgg tctcggcttc cagttcgatg cccattgcct
     3301 cgacgtcgcg gctgatcttg atgcgctggg cttgcgcgtc gcgtttgcgc aaagtccaca
     3361 gcagctgttg ggttcgtttg aggctggcct cgaagccgcg gtatttaccg gccttttctg
     3421 cctgcaattc caggtgctcg agttgtttgc ccagctcctg gcggatatcg tccacgcgca
     3481 acagattttc gcgcgtatcc tgtaacctca gttcggtctc acgccggcgt tcgcgatatt
     3541 tcgacacgcc ggcagcttct tcaagaaagc tgcgcaggtc ctccggcttc gcctcgatga
     3601 tgcgggagat catgccctgt tcgatgaggc atagccacgc ccacccaggc cggtacccag
     3661 aaaaatatcg gcgatgtcgc ggcggcgaac atgcaggttg ttcaggaagt aagacgattc
     3721 gccgtcccgc tccaacacgc gcttgaccga aatttcggcg tacgccgacc acgggccggc
     3781 cgccttgccc aggctgttgt cgaaaataag ctccacgctg gcgcggccga cgggcttgcg
     3841 ctcccccgag ccgttgaaga ttacgtcctg catgtgctcg ccgcgcagtt gcttggcgga
     3901 cgattcgccc agtacccagc gaatggcatc gatgacgtta gacttcccgc aaccgttcgg
     3961 gccgactacg ccgaccagtt gcccagggac tggaatgtgc gtggggtcaa cgaaggactt
     4021 gaaaccagct agcttgatgt gtgtgagtcg cactgtcggg gctttataaa agcttataat
     4081 ttcaacggat actcataaaa caaacgcctg gagccccaag acaacggacc aggcgcacgt
     4141 tctttcttcc cgcattttaa caatggaatc gtcatccgag aatgccgagc acgccttctt
     4201 tatcactcga cacctttccg aatccgaatc cggcgcgcga ttatctgatt catatggaaa
     4261 ttccggaatt cacctgcctg tgcccgaaaa ccggccagcc ggacttcgcc acgctgttgc
     4321 tggattacgt tccggacaaa aaatgcgttg aattgaaaag tctgaagttg tacgtctggt
     4381 cattccgcga tcaaggcgcc tttcatgaag cagtcacgaa ccgcatgctc gacgatctgg
     4441 ccgaggcaac acagccgcgc ttcatgcggc tcacggcgcg gttttacgta cgcggcggga
     4501 tctttaccac cgtatcggcc gaacatgtaa aaaacggctg gaagccgaag ccgcgcatag
     4561 atgtagcgca gtttgaatcg gactcaccgg tgcgttgaag gctccaatcg tccggaatac
     4621 aacgcagcgg ctaccagcat ggcgccgccc accaagtccc gccagttaat gtgttcgaat
     4681 gccagcagat aagaagaagc agcggcaaat accagctcgg acagcatgat tacgatggct
     4741 ctgttggcgc tcgtatgcgc aagcccgaac tggaccacca gattgacaca gaacaacacc
     4801 agtgccacca gcccaaccag caaccaggcc gccgcatcga cactggcgaa tgcactcacc
     4861 ggttgttcgg tcagcagcgc cacgaccagt gcgaccgcac tgacaccagc ccatactgct
     4921 ccggccttga tcgtctcgtc aatgtgaacg gccttgcggc tcagcacatt ggaaagcgcg
     4981 aatgccatgc cggagcccag tcccaaccat tccgcgctgt tgtaaggcaa cggccagcgt
     5041 ccgcccggtt gccacagcat caccaccgcg ccagcgattg acagcgcaat gacgggatag
     5101 ccgcgcgggc ctgcgacttc accaagcagc agccacgcaa acaccagggt ccacagcggt
     5161 gccaggtaaa acaacagcat gacgcgcacg acctcgccgt acagcatggc tagcacgtag
     5221 ccgacattgc acagccctgc ggaaatcgcg atcgcaacca gcaaccacgg cgccttgcgc
     5281 agttgcacgc cacgctttcg aaacagcagc gaaccggcca gcaacggcaa taaataagtg
     5341 accaatgaag cggatgcacc gcccagcccg gctgactgca aggcccgata gggataccag
     5401 atcacacccc agaccagcgc accggtcaga aggctggcaa ccgccaccat gttgttcttc
     5461 tgtgctgtca tgcccgcctt tataatccga ccgccctgtg accacgatgc agtctgccga
     5521 aatgaatgca aatctttccc gacttcaacc ttatccgttc cagaagcttg cggctttgct
     5581 ggccggcatc acgccggacg ctgccctgcg cccgataagc ctgtcgatcg gtgaaccgaa
     5641 acatcccact ccgcaattca ttcgcgacgc attaatccag cagctcggcg gtttgtccag
     5701 ctatcccgcc accgctggca gcgaagcgtt acgcagtgcc atcgcgcgct ggatcgagca
     5761 gcgctaccgc atcgacagca tcgatgctgc gaaacaggtg ctgcccgtca atggcagccg
     5821 cgaggcgttg tttgcttttg cccaggcagt ggtcgacaca tcagacaata ccccagcggt
     5881 cgtggcgcct aatccgtttt accagattta tgaaggggcg attatcctcg ccggcgcgga
     5941 gcctcatttc ttgaatctgt taccggagaa cggttttgcg cttaacctgg gccagatcgc
     6001 gcaggaaacc tggaagcgta cgcagcttgt ttatgtctgc acgcctggca acccaaccgg
     6061 caatgtcatg aacatggaag aatggagcca gttgttcggg ttgtccgacc gttatggatt
     6121 tgtcatcgcc tcggacgagt gttattcgga aatctacttc gatgacaacc ggccgccgct
     6181 gggcggcctc gaagcggcct atcgcctcgg acgcaaggat ttccgccggc tggttgcgtt
     6241 cagcagtctg tcgaaacgct ccaacgtgcc gggcatgcgc tccggctttg tcgcgggcga
     6301 cgccgaaatc ctggcgaaat tcctgctgta tcgtacctat cacggctgcg cgatgaatcc
     6361 tgcagtccag gccgcgagcg ccaccgcgtg gagcgatgag caacacgtcg cggaaaatcg
     6421 ccggctctat cgggaaaaat tcgatgaggc gcgcgccatt ttgtcgcccg tgactgaagt
     6481 taacatgccc gatgcggcgt tctatctctg gctgcgaacg ccgatcgccg acacggaatt
     6541 cgcaagacaa ttattcgagc aattcaacgt gacggtcgtc cccggcagct atctcgctcg
     6601 cgaagcgcac ggcgtcaatc ccggccgcaa tttcatccgc gtcgcactgg tagcggcggt
     6661 cgaagaatgc gtcgaagcgc tgcaacgcct ggcggctttc tacggacagt tttcagcggg
     6721 caaggacagc aatgatgcag tcgcatcact gaacacgcgc cgccaggcta cgccgtagct
     6781 caagctcatg ctcgctcacg cagagcgccg agtggaagag acccgtgatc gcattgctcc
     6841 gtttcagtga agttatatag cgcgtcgcta caccagcagt tcgcacggca aggaaaaaac
     6901 ccgcagcggg atttttcctc cgtgttgttt ctgcatcgcc ccgtcgcgga aacttctgag
     6961 cccggccacg accgtttcag cctttgaatc cggcaacgcg gtcagaatca tatcgttgta
     7021 ggcaggccag gacagcgagt gaaatgcggc accggcttcc cctacgccag taacctcatg
     7081 catttcggtg aaggccctga cgccgtgctc tttcaggaga cctttgattt cctcctcgat
     7141 ggactccctg aacacaatca tcagcatttt catgtgagct tcctcttcgg tggcgaatcg
     7201 acagtatgag caacgggttc cgtgcgctgg ttgaccatga ttgatatatt acgtttcacg
     7261 tcgcagtcgc catcaaacac tgcgggagga gtgcaaactc aatacgctgt tacggcttcg
     7321 gggggaaacc cgaatcccga ttgattcgag caacctgcta ctcgatccaa tactctccga
     7381 cagattggag gtttgagagt gaatttgccc ctgcctctcg tgcgccgcta ccggtttgct
     7441 ggccgcgaat gccgcggaaa cctttcgttc caccgataaa cgtttcgacg aaagaatact
     7501 tgaacgactg acgcttgccg tcgcctccca ttttggattg cgtggtgcca gtgccgcggc
     7561 tgaatatctg gctaccgtct tccagcgagt acaccgtata agtaacaaac gatccgctcc
     7621 aattagtgta gtcactcgtg ccgcggacat ggctttccgt aacttttact ccagcgaatt
     7681 ccaggtcgtt tgcaggatag gtgttgcgca attcatagat gcgcagttga tgcccgggcc
     7741 ggtcgccgac gtcaatgatt tgctcctgca gatatttgct ttcggatttg gggccttcag
     7801 tgatgctgta cttctgctgt gcccaggtcg atgaagcggc gaacaggacg accagtgttg
     7861 ccaatgtcag gtacatgttc ttcatgacaa tctccccttt ttagaaatag ttctgttgat
     7921 cgaaatgccg ggcagacaag tgctcaggcc cggttacgtc gattgctttg attgctaagg
     7981 aggcgaagaa ttacgccgag tggagcatga acgctcctta agacccggcg atccggctga
     8041 tgctgaacga catccggatc tggctttgag tacgcccgcc tgcatggcgg ccggtttctg
     8101 attgcttagt ctgacaacgt catatttata ggcttattcc cctcgtctcg caatgccttg
     8161 gcgggctgcc ggtgattcgc ggacaccgct tggcgtttac ccgtttatgg tcgcagaggg
     8221 acgctcgttg cgcgggtttg tcgtgccgca ctaagatagc gggcaaatgc cggatgcaac
     8281 cttcgacgaa cgatatctca acagtctcgc cgcaaccggg accgtcaggg cttatgcaaa
     8341 aaatacctta ttgatccagg aaggagattt tagtgatcag ctctacgtgg ttctggctgg
     8401 ccgtctgaaa gtcttcctct cagacagcga cggtaaggaa gtcatcattg acacacttcg
     8461 gcagcgtgac tgctttggcg agatggcgct cgagggagaa ccgcggtcgg cgtccgtcat
     8521 gaccttggag ccgtgcaagc tcgccgtaat cgagcgcgat cagttcaaac agtttcttag
     8581 tctgtacccc gaggccgcct acgcgttgat cgtgaccgtt attcgccgcg cccggaatct
     8641 gacacgcgcc attggaagcc tggccttgct ggacgtctat ggacgtgtag cacgtctgct
     8701 gctcgacaac gctagcgagg agaacggcaa actcgtggtt ggcgaacgca tgacacaaca
     8761 ggacatctcg aagcgcatca gttcgtcgag agaaatggtc tcgcgaattc ttaccgattt
     8821 gcgagaaggt ggctatatag gtatcgaaga agatcgaatc gtgattcttc gggcgcttcc
     8881 tgcgcgttgg taaattcttc ggaatgtatg gaccacgtag gtcttcaaac gacagcgtct
     8941 aatttagcaa tcagcgccgc ggcctcttgc agatcacctg agtcgtgacc ttcggtgaac
     9001 cagtcgtaga cgggtcgaat taaattaagc gcttctttgc atcggctttg gtcccttaat
     9061 acccgcgcat aactgattgc ggcgcgaagc tcccaagatt tagcggactg ctgtcgcgca
     9121 accagcaaag cctctttaaa aataacttcg gccttctcga catttcccat ttccccaaat
     9181 atgcaggcct tgagccgtaa tacattgctt agcgcacccc tctcctggca ccccgtcttg
     9241 ttaatttgct ccaacgcttt ctctaacaga agcaaggccg taccgggatc tccgcaaaaa
     9301 cggagggtct ctgcgtgcat agcgttgata gtgggcatgt agatgcccat tcctgccgct
     9361 tgccattctg gaatgacttg tcgaaacttg gcgtctgcct ccttcgcccg atgacaacga
     9421 agaagccaga atgcgcggct caaggggcaa tacacctgcg tgaaaaaaac cagtttttgg
     9481 tccagagcaa acctctcaat ttcgttgaga aattcgtcca ttagcatcgt atcgctggag
     9541 tccgtcagca aagtgtgagc caagaatgta tgcacccagc aaaaatcgaa gacgtgcttg
     9601 cgttcgcgtg agttcgcgac tgctaatttg gcgcttgctg cggctcgatc tggaaagccc
     9661 agccgccact cggctgctgc cctgtaggca agcgctattg ttttgggatc atgattaatc
     9721 aggtccgcaa cgccgcggtc tcgttctggg ttatagcggg caaggataat gtcggcatga
     9781 tagatggacc gcgaaaagtt gcccaaaaaa taatgcgaat cgcatgccgc ccaatgcccg
     9841 gcgagccgca tgctctcgct gtcccgatct tctgcaacat caattagtcg cttggcccat
     9901 gataaagaat cctctacgcg acccgcgcat agtgtgtaga cccagaggcc ccacaatacc
     9961 cgcagcgtgt agtccccatc gtctagcgac tgatccagcg ccaatgcagg ttcaagattg
    10021 gcagcgactt gcgggtgggt atagccgtgg agttctaccc aagccatgcc aagtagcgct
    10081 cgcaattgta gttcccaggt atcgcgctgc cgcgacggcg caagccggga ggtcacttcg
    10141 agaccgcgtt cgaaatgtcc gatcgcttcc tgaagtgcga cgcgcttctg cgctagttca
    10201 ccggcttttt gccacaaggg aatggcggtt tccagcatgc cggcttccgt atagtgctgc
    10261 gccagcaatt ctggttcagt ccggaccctg gaaggaaagc gttcgctgat ggcttcagcg
    10321 atttgcccat gcagtagctg ccgctgagtc ttgagcaaag aatcataagc agcgtcccgc
    10381 accagggcgt gtttgaaagt gtatgtggct tccggtggcg tgccgcgccg ggaaacgagt
    10441 tccgattcag tgagtttgtc cagcgcctcg tggaactgag cctcactcat gcgcgagacc
    10501 gcacgcgcta attcatagct gaactcgcgc cctaagactg cgcccacttg ggcaatttcc
    10561 tttaagggaa tcaggcggtc caagcgagcg gcgagcgaat cccgcagtgt ggctggaatc
    10621 gcgagctcac tatgggttcc ggaatactcg tagcgctcgt ctacttcgat tagcagatcg
    10681 gactcgagca ccgacttggt aagttcctcc aggaataacg ggaccccgtc agtcttatca
    10741 acaatgtggc tgaccagctc cgctggcaat ggcttgtcct ctgcgacccg caggacgatt
    10801 ccggctccct gcacccggct caaccgtcga agcccaatgc gtgttacatg cggcacatcc
    10861 atccaggccg gtgcaaattc gggccgatat gtaattaaaa ctagtagcgg gatcttgttg
    10921 gcggagtcga tgagcgcgtt cagcacttcg agcgtgcttg gatcggccca gtgagcgtct
    10981 tcgaacaata atacggtcgc ctgcttccgc gcgatggccg ccacgatgtt cacgaggagt
    11041 gcaatgatct cctccttccg gcgttggggg ctcatatcca ggacaccata gcggtcgtcc
    11101 ccggtaatgg acaaagccaa cgccagcaaa tggcaatcgg tgcggctgtg ccccagttca
    11161 aggatcagcc tttgctcgat tttgttgacc ttttgtgcgg cagtatcgtc acgctgaaaa
    11221 tcaagcgcac gttccagatg attcgcaatg ggataaaggg cgctattaat gtgatacggg
    11281 gaacactgat agtggagcgc tatttcgatg ttttctccca actgctgacg gaatgcggtc
    11341 agcatgcgag attttccgat accgggctct ccttgcacga gtactacctg accctggccc
    11401 gtacgcgaga gcttccaccg atcccgcaga agcgcaattt cctgctcgcg acccaccatg
    11461 ggggtgaggc gcccgcgcgt agccgcatcg aaacggctgg tagcggcact gggacccaat
    11521 actttccacg cccgcgtgag ttgcgcaatg cctttgaggg cgtgcaggcc cctatcttca
    11581 taatcgaa
//
LOCUS       DATMZQ010000117         4929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1427384, whole genome shotgun sequence.
ACCESSION   DATMZQ010000117 DATMZQ010000000
VERSION     DATMZQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427384"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VN496_03705"
     CDS             <1..595
                     /locus_tag="VN496_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16081.1"
                     /translation="IVQFANAELVADRTYLLSRLLRGQAGSEWAIGAHLPAGASFVLI
                     DQHLVTIARGQDALERSLQLRVVMAGRDHGDPTALALSATPHATALKPLMPVHLRATR
                     GGSGITVSWIRRTRVDGDTWVGEVPLGEDGEQYAIDILSGGTVVRTLTTTTPSALYAS
                     ADEVSDFGAPQASLHVRVTQLSATVGRGIAADAVLTI"
     gene            592..1308
                     /locus_tag="VN496_03710"
     CDS             592..1308
                     /locus_tag="VN496_03710"
                     /inference="COORDINATES: protein motif:HMM:NF022436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HXF16082.1"
                     /translation="MKEIMTNTPRLALPAIEAAQAQKHVTHNEALTLLDALTQLAVES
                     RTLTTPPGTPADGACYIPAAGATGAWAGWSGQIALFSGGGWIRVVPVSGVKAWVKAER
                     LTITYEDGIWRDGIALTVHGGRVTLRTKEEEITLAGAFVDTTDAAFIPDRAVVLGVAS
                     RTTLAITGATSYGIGIAGNTTQFGNLLGIALNSANNGVIGPTAFYADTRVRVTANGGS
                     FTAGKVRLIIYFLEMSIPSS"
     gene            1313..1864
                     /locus_tag="VN496_03715"
     CDS             1313..1864
                     /locus_tag="VN496_03715"
                     /inference="COORDINATES: protein motif:HMM:NF017638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HXF16083.1"
                     /translation="MQSTFETCLSLLLAHEGGYTNHPSDPGGPTNFGITIEDYRRYVK
                     VNATAADVRRMKLDEAKIIYRDKYWDAQRCDELPAGVNYAIFDYGVNSGTGRSGKVLR
                     RMLGLPDDSSTVTDAVIAAARAADPKILIAAICGERLRFLQALKTWPVFGAGWGRRVA
                     EVKSTALKMAADAAKASTMARKK"
     gene            1893..2123
                     /locus_tag="VN496_03720"
     CDS             1893..2123
                     /locus_tag="VN496_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16084.1"
                     /translation="MWNKIRSWFGNSVTILWARVQVLGGLTAASLMALASDPNVTGTI
                     QSALQPKFIPYYVIAVGLITEIARRRTAGSSK"
     gene            2124..2528
                     /locus_tag="VN496_03725"
     CDS             2124..2528
                     /locus_tag="VN496_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16085.1"
                     /translation="MFSAVLSWLGNLFGGPFAKAAVDAYRAKLAAENTSEKIAADLAA
                     RELSVEQRERELAAEMLIAEQGRWYTALPRPLFAAAFIIYVWKVVVWDKVLGLGATDA
                     LSGDMSQWAMAVVTAYFGGRTFEKVARVFAKK"
     gene            2684..3070
                     /locus_tag="VN496_03730"
     CDS             2684..3070
                     /locus_tag="VN496_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16086.1"
                     /translation="MAHVLEYLPKVENWKNWKNEKRLPYQGVVKHDWVPTGRIDFATR
                     LNGSAEEAEQPSEFKLLVEERRIVESIAGNENLEIQWRLATLKEAKAVVTQYHKYLSE
                     NALIKSIFDDTIALPPPKNNSANSSA"
     gene            complement(3116..3559)
                     /locus_tag="VN496_03735"
     CDS             complement(3116..3559)
                     /locus_tag="VN496_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16087.1"
                     /translation="MIEPIMYMGIGFLLAALIAVAVIPLVHDRAVRLTTRRLRAALPL
                     SMKEIQAGRDLLRAEFAMATRRLEISLKQLKDKSTGQVVELSKRDDVINRLKLQRYTL
                     NVEVIALKSQLEALKTRPVPAAKGAGAKPNVLSAMRHWIPQRSYR"
     gene            4553..4699
                     /locus_tag="VN496_03740"
     CDS             4553..4699
                     /locus_tag="VN496_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16088.1"
                     /translation="MTDTNIEKNEESLLTYEVSDEALETAVGSIIAGNYTLAACTGLS
                     VCPG"
     gene            4729..4902
                     /locus_tag="VN496_03745"
     CDS             4729..4902
                     /locus_tag="VN496_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16089.1"
                     /translation="MSNSFLNWSGRADIEGRVTERRCPPNRDEPTTPGDYHLPFAQAK
                     AKAAKWVWQAYLI"
BASE COUNT         1122 a         1381 c         1430 g          996 t
ORIGIN      
        1 gattgttcag ttcgccaatg ccgagttggt cgccgaccgt acctatctcc tgtcacggct
       61 gctgagaggc caggctggca gcgaatgggc cattggtgcg catctgccgg ccggggcatc
      121 gttcgtgctc attgatcagc atttggtgac gatcgccaga gggcaggatg cacttgaacg
      181 cagcctgcaa ttgcgcgtcg tcatggcggg ccgcgaccat ggtgatccga ccgcgctggc
      241 gctttccgca acgccgcacg caactgcgct caagccactg atgcccgtac acctcagggc
      301 tacgcgtggc gggagtggca ttaccgtcag ctggatccgc cgcacgcgcg tcgatggcga
      361 cacctgggtg ggcgaagtgc cgctcgggga ggatggggag cagtacgcaa tcgacattct
      421 ttccggcggc actgtggtcc gcacgctcac aaccaccacg ccatccgcgc tttacgcctc
      481 cgccgacgaa gtgtccgatt tcggtgcgcc gcaggcaagt ctgcacgtgc gcgtcacgca
      541 gctttccgca accgtcgggc gcggtatcgc ggccgacgca gttctaacca tttgaaagaa
      601 atcatgacca acactcctcg tctcgccctg ccggcgattg aagccgcgca ggcgcagaaa
      661 cacgtcaccc acaatgaagc gctcacgtta ctcgacgcgc tcactcagct ggcggtcgaa
      721 agccgcacgc tcacgacacc gcccggcacg ccggccgatg gcgcttgtta cattccggcc
      781 gcgggcgcaa ccggcgcatg ggcgggctgg agcggacaga tcgccctctt tagtggcggt
      841 ggttggatcc gcgttgttcc cgtgtccggc gtgaaggcct gggtgaaagc cgagcggctt
      901 acaattactt acgaagacgg catctggcgt gatggcatcg cgctcaccgt gcatggggga
      961 cgcgtcaccc tgcgtaccaa ggaagaagaa atcacgcttg ccggtgcttt tgttgacacc
     1021 acggatgcgg ccttcattcc ggaccgcgcg gttgtcctcg gtgtcgcctc gcgcaccaca
     1081 ctcgcgatca ccggcgcgac ctcgtatggc atcggaatcg cgggcaacac cacgcaattc
     1141 ggaaacctgc ttggtatcgc actcaactca gcaaacaacg gcgtgattgg gccaaccgcc
     1201 ttctatgcgg ataccagggt gcgggtcact gccaacggcg gcagtttcac cgccggcaaa
     1261 gtgcgcctga tcatctactt tcttgaaatg tccattccga gctcgtgacg caatgcaatc
     1321 aacttttgaa acctgcttat cgttgctgct cgcacacgag ggtggttaca caaatcatcc
     1381 aagcgatccc ggcgggccaa ccaatttcgg catcaccatt gaggattatc gcagatacgt
     1441 gaaggtgaat gcgaccgccg ccgacgtgcg cagaatgaag cttgatgagg cgaaaatcat
     1501 ctaccgcgat aagtattggg atgcgcagcg ctgtgatgag ctgccggccg gcgtcaacta
     1561 cgcgattttc gactatggcg tgaactccgg cacagggcgc agcggcaagg tgttgcggcg
     1621 gatgctcggg ttgccggacg actcgagcac cgtaaccgac gcggtcattg cagccgcgcg
     1681 tgcggccgat ccgaaaatcc tgattgccgc catctgtggc gagcgcttgc gctttctcca
     1741 ggcactcaag acatggccgg tcttcggcgc tggctggggt cgccgtgtgg cggaggtcaa
     1801 atcaacggcg cttaagatgg cggcggacgc tgccaaggcc agcacgatgg cccgcaaaaa
     1861 gtaattcaag gcaaattcgg agaatacaat cgatgtggaa caagatcaga tcctggttcg
     1921 ggaactcggt cacaattctt tgggcacgtg tgcaggtgtt ggggggtcta acggctgcgt
     1981 cgctgatggc tctcgcgtct gaccccaatg tgaccggcac aattcaatcc gcgctgcagc
     2041 caaaattcat tccgtattac gtcatcgctg tcgggctgat cactgaaatc gcgcgccggc
     2101 gtacggcggg gagctcaaag tagatgtttt ctgctgtcct ttcctggctg ggcaatctgt
     2161 ttggcggtcc gtttgcaaaa gcagcggttg acgcctatcg cgccaaactg gcggctgaaa
     2221 acaccagcga aaagattgct gccgatcttg ccgcgcgcga actctcggta gagcagcgcg
     2281 agcgcgaatt ggccgctgaa atgctgatcg cggagcaagg gcgttggtac acggcattgc
     2341 cgcggccgct gtttgccgcc gcattcataa tttacgtctg gaaagtggtg gtctgggaca
     2401 aggtcctggg cctcggcgcg accgatgcgc tctccggcga catgtcgcaa tgggcgatgg
     2461 cggtggtcac cgcatatttc ggcggacgca ctttcgaaaa agtcgcgcga gtttttgcga
     2521 agaaataggc atggcgtgcc acatcccgtt accgttttgc agaaacggcg gagagcgtgc
     2581 tcgcagtcgt caatgaaacc acctgggctg gcccggcgtt aagagatgtg gggatttgcg
     2641 tgtgcatccg aaatccggtc cgcgtttgtg aaaggagaaa aagatggctc acgtgctgga
     2701 atatttgcca aaagtagaaa attggaaaaa ttggaaaaac gaaaaacggc taccttacca
     2761 aggggtcgta aagcatgact gggtgcctac cgggcgcatc gatttcgcaa cgcgcctgaa
     2821 tggaagcgcc gaagaggccg agcagccaag tgagttcaag ttgcttgtag aagaacgcag
     2881 gattgtcgag agcatcgccg gaaatgagaa tctggagatt cagtggagac ttgcgacgct
     2941 gaaagaagca aaagcagtcg taactcagta ccacaaatac ctgtcggaaa acgcacttat
     3001 caaatccata tttgacgaca ccatcgcatt gcctccacca aaaaataatt cggcgaattc
     3061 ttcggcgtag tggtgcgatt ctaaaagcgc gacgccaaaa ttgcgtcagc aggcactagc
     3121 gatagcttct ttgcggaatc cagtgccgca tagcggacaa gacattgggt ttggctccgg
     3181 ctcccttggc ggctgggacc ggccgcgtct tgagcgcttc aagctgggac ttgagtgcaa
     3241 tgacctcaac attcaacgtg tagcgctgga gctttagacg attgatcaca tcgtcccttt
     3301 tgctcagttc gacaacctgg ccggtgctct tatccttgag ctgcttgagg ctgatctcaa
     3361 gtcggcgcgt ggccatagca aattcggcgc gcagcaaatc cctgccagcc tggatttcct
     3421 tcatcgatag cgggagggcg gctcgcaggc ggcgtgtagt cagcctcacc gcgcggtcgt
     3481 gaacaagagg tatgacagcc accgcgatga gggcagcgag taggaagccg atccccatgt
     3541 acatgatcgg ttcaatcatt tcagaaaaac cgccaactca gtcattcggt tccaaggcta
     3601 gccgctagtt cggtcagtgg tgttggccga aaacccccag aaggcgacca accatgaaat
     3661 acagtgtgca cgcgccccat accaaacttg cgctgatgag aatggcgaac agcgtttcga
     3721 taaatccagc tgaaaaattt aacaaaaaac tgtgcaccgg ccaaagtgtt ccggtaacgc
     3781 acccaaacag aagaatgatt cccagcaata tcagtgacgt cggaacggac aggtggagtt
     3841 tcatatctca cggtccagcc atgcacggca ctgataaata agccgcatcg aagccaatgc
     3901 acacaacacc ctgacaccgt tcgcgcaaca cagttaacca gagagctgtg gtgtatcagg
     3961 tcgagaaacg tccgagcttg tcgactggcc gggcgaaggt gcgtcaggtc ttttcaagga
     4021 cagtagtacc tccaacctct ttcaatatac gtcgcgcggt gcagtgttac tgcactttcg
     4081 tggcccgact cattgataac gggcgacatc gttccgttac cttggacgcg aactgcattc
     4141 acaggagaag ttgatggacg ttccgacaac tgcggcatcc gccgagaaat catttgagtc
     4201 ggacacggct gaagctatct gcaagttact agagcagacg atgctgagat attttcctca
     4261 aactgagaac gatgctgctc agcccaaacc gtcctcggtt ccgctggttc ctcaccggca
     4321 agagaagccg caaatctagc gtgcattgct tccagaacag tgcagccctt ttatcttggg
     4381 gccgtacttc ccgctggaag ggcggacgat ggagcttttc aggagagtcc aactgtagcg
     4441 cgataccaaa cgcaagtttt gtgaccctcg tcacatgcgc tatcgcacga gcgatcctat
     4501 ttcgaaatga aggctgagga ttgggacacc catccacagt aggacaaaga ccatgaccga
     4561 tacgaacatc gaaaaaaacg aagaaagtct ccttacttac gaagtttccg atgaggcgct
     4621 tgagaccgcg gtgggaagca taattgcagg aaattacacg ctcgcggctt gtaccggcct
     4681 gtcggtttgt ccgggttgat cgctcaacac caacaattgg aggcatccat gtcgaacagc
     4741 ttcctgaact ggagcggccg ggccgatatc gaaggacgcg ttacagaacg tcgttgcccc
     4801 ccaaaccggg atgagccgac gacgcccggc gattaccacc tgccgttcgc ccaggctaag
     4861 gctaaagcag caaaatgggt ttggcaggct tatcttatct gattggcgcg caggaccgcg
     4921 tccaaaggg
//
LOCUS       DATMZQ010000118         5931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3499623, whole genome shotgun sequence.
ACCESSION   DATMZQ010000118 DATMZQ010000000
VERSION     DATMZQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499623"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VN496_03750"
     CDS             complement(<1..559)
                     /locus_tag="VN496_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXF16090.1"
                     /translation="MKQQQLVTDVAAIRRAYGQFLSKGRVPHGVVPDFIERSWKRSAA
                     FGVAHDRIREIGRVDETDLRIVVSQFGSLIDLATPVMQHLHHQLTGTGSVVLLCSGTG
                     FILHSMGDPDFLPKTQHIALKPGVSWAEQDKGTNAIGTAIVERRPIVVYAGHHYIRTN
                     HFLACSATPVLDPFGDVAGVLDITCD"
     gene            complement(950..1339)
                     /locus_tag="VN496_03755"
     CDS             complement(950..1339)
                     /locus_tag="VN496_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HXF16091.1"
                     /translation="MVKRPQDTSTADMDVIFVRDIQLDTLIGAYEFERRQPRTLKVNI
                     EIGRWSLTACKTDRLADTIDYAAVVEVVRDSFASHQFHLLEPLAELIAGCLLEKFDAQ
                     WARIELSKSGVVPSARDVGVRLERRRG"
     gene            complement(1341..1718)
                     /locus_tag="VN496_03760"
     CDS             complement(1341..1718)
                     /locus_tag="VN496_03760"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXF16092.1"
                     /translation="MGESAEKAYAEAEIKQRLAAELPHWYYEDGWMRRKFKTGGWKAT
                     LMVVNAVGHLAEAAWHHPDLTVSYAFVIVKLVTHSAKGITEKDFALAKKIEEVVQWQP
                     GKEGGALEGTPKEDQRFKYIKYD"
     gene            1905..2462
                     /locus_tag="VN496_03765"
     CDS             1905..2462
                     /locus_tag="VN496_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HXF16093.1"
                     /translation="MVKSEASRVAWVLTGSGHYLKECMEIVGRLPAVDLFLSEAAGEV
                     LKMYGYDLATLRRGMKVFRDNTASSVPVTFLYGGNYHTVVIGPASSNTVAKCVAGISD
                     TLATNIYAQAGKCRIPSIVFACDTAPALISEAPGGDVWVYPRAIDLDNVNRLAKFDQT
                     TVVVSVEELGTALDQRLSCLSTSSS"
     gene            2438..3835
                     /locus_tag="VN496_03770"
     CDS             2438..3835
                     /locus_tag="VN496_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HXF16094.1"
                     /translation="MSEHILFLTGRLAEKNLHRVIEAMQPAPFQHSVLALSLQVAGLM
                     TSDMIRRRLPALAGIDRVMVPGRCRGDLNELSSHFGVPFERGPEDLKDLPRHFGREAA
                     PRDLSRHDVRIFAEIVDAPNMSLTEILDQANAYRNAGADVIDLGCLPETPFPQLEDAI
                     GALHASGFPVSVDSMDAEELLRGGRAGADFLLSLKPETLWIADQVASAPVLIASTPND
                     MGSLYQAIEAMEKKGRDYLVDPILEPIHFGFTESLVRYRDLRKRLPDAKILMGTGNLT
                     ELTEVDTLGVQTLLMGIISELQIKNILTTQVSAHCAGVIREADQARRIMHSARDAGDL
                     PQGFGGALLALHSLDPFPWSAQEIAATAAEIKDPSFRVQISPDGMHVYNRDGMRTATD
                     PFKLFAHLALEQDGSHAFYMGVELARAQIAWQLGKRYVQDSELEWGIAQLRASSDAPV
                     TLVARPVTSKTKAVP"
     gene            3832..4404
                     /locus_tag="VN496_03775"
     CDS             3832..4404
                     /locus_tag="VN496_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HXF16095.1"
                     /translation="MIFETIVSTRDVAGSAHIAPMGIRREDGFIVLSPFRPSTTLEHI
                     LREQCAVVNYVDDVRIFAGCLTGRYQWPTQSAERLTCPRLSAALAHSEVNLERVQDDP
                     TRPRLYCREILHATHGPFRGFNRAQSAVIEAAILVSRLNMLPQEKIDAEMAYLAIGLE
                     KTAGPRELEAWGWLKERIEQFRSARKESAA"
     gene            4407..5117
                     /locus_tag="VN496_03780"
     CDS             4407..5117
                     /locus_tag="VN496_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HXF16096.1"
                     /translation="MMLASVRSLDEALSALDSGADLIDLKEPSRGALGALDHAAVRIC
                     VQAIGGRRPVSATIGDVPSMEPSAVRDAVECMAATGVDFIKIGFFFHPHASDCVSALS
                     RLAREVRLVAVLFADAPYDAELIDLLARRGFSGAMLDTAQKNGKTLRDWRTMRELGKF
                     VKHVQDLGLLSGLAGSLRSNDIVPLLELAPDYLGFRTALCADWDRRKSLDCGAASRIR
                     ALIPAHHGQELTIRELRV"
     gene            complement(5127..>5931)
                     /locus_tag="VN496_03785"
     CDS             complement(5127..>5931)
                     /locus_tag="VN496_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXF16097.1"
                     /translation="LEALVAAQEKDDEGPTDEEISANVMTMLLAGEDTTANTLSYMMH
                     FLMEFPNVQAAVQEEVDTVLGMADQPWQDPATADKLRYIEAFTNEAMRCKPVAGHVTF
                     LEPNEDVQIRDVFVPKGTPILALNAHLGVQEANFSDANEFRPERWLQNSGDPERAHNA
                     KAFIPFGAGPRLCPGRQLAMLQIKMVIAMLCRDFEVSRPKDAAPMEEIYNFTVGPTSV
                     LTTLRPRRPVRRGIDIELRDGNRRTFVLPISFPERRVADRRREHTSSAT"
BASE COUNT         1222 a         1712 c         1723 g         1274 t
ORIGIN      
        1 agtcgcacgt tatatcgaga acccccgcaa cgtccccaaa tgggtccagt accggagttg
       61 cagaacaagc caggaaatga tttgtccgga tgtaatggtg ccccgcataa accacgattg
      121 gccggcgctc gacaattgca gtgcctattg cattggtacc cttgtcttgc tcggcccatg
      181 agactccggg tttaagcgcg atgtgctggg ttttgggcaa gaagtcggga tcacccatcg
      241 aatgcaggat gaagccggtg ccgctgcaca acagcacgac gctccctgtc ccggtaagct
      301 gatgatgcaa gtgctgcatg accggtgttg ccaggtcgat caggctgcca aattggctga
      361 cgacaatgcg caaatccgtt tcgtcgaccc gtccgatctc gcggatgcga tcatgcgcta
      421 cgccaaaggc agcgcttcgt ttccacgatc gttcgataaa atcagggacg accccgtgcg
      481 gtacccgtcc cttgctgaga aattgtccat acgcgcggcg gatcgcggcc acgtccgtga
      541 caagctgctg ctgtttcatg cgacctcctc ccccgtctcg gcgcgtaagt caccggacaa
      601 gagtattgcg ttaccggagg tcgtggtgga accctctcct gtttgccatg cgttcacaac
      661 cacgaaccgg gtggggtagg ttcccgcgtt ctttacgacg ctaaccttca tacaatcaca
      721 gacgcactgg cgaggtcaac gcccgttgca gcgttcgcaa gcccgttctt gcagtgcaat
      781 aagccgtgtc caaaaaatat ggatcgagca ggaggcgtgg ccaaccgcag cgaatatatg
      841 tgtttccccg gcttcggata agaccacagg ctgcgcgctg tccggtagtt cagcgatcgg
      901 agttgcacag cgccatcgcg ctgcaataag tcagaacgcc tggattcgcc tatccccgcc
      961 tgcgttcaag gcgcacgcca acgtcgcggg cgctcggcac gacgccgctc ttggaaagct
     1021 caatgcgcgc ccactgcgcg tcaaattttt ccaacaggca tcctgcgatc aattccgcca
     1081 gtggctcgag cagatggaat tgatgcgaag caaagctgtc tcgcaccact tccaccacag
     1141 cggcgtaatc aatggtatcg gcgagccggt cggtcttgca tgccgtcagg gaccaacggc
     1201 cgatttcaat attaactttt agcgtgcgcg gctggcgccg ttcaaactcg taagcgccga
     1261 tcagcgtgtc gagttgaata tcccggacaa aaatgacatc catgtcagcc gtcgatgtgt
     1321 cctgaggccg cttcaccatt tcagtcgtac ttgatatatt tgaagcgctg gtcttctttt
     1381 ggggtgcctt ccagcgcgcc gccctctttg cccggttgcc actgcacgac ttcctcgatt
     1441 ttcttggcca gtgcgaagtc cttttcagtg atgcctttgg cagagtgtgt aaccagcttg
     1501 acgattacga atgcatacga aaccgtgagg tccggatgat gccaggcggc ttctgcgaga
     1561 tggcccacgg cattgacgac catcagcgtt gccttccagc ccccggtttt gaatttgcga
     1621 cgcatccatc cgtcctcgta ataccagtgt ggcagttcag cggccagtct ctgtttgatt
     1681 tcggcttcgg catacgcttt ttcggctgac tcgcccatga ggttctccgc aaccagagga
     1741 taggggtttc gaaagcattc tagccggata tcccggcgac acgatgtgtt ccgttcgccc
     1801 tcaaacacga ctaacatcct tattgtgagt cgaaaccctg cttgcggctg ccgacggcgc
     1861 gcaattacga taaccagtct gttgtccaga gctggtagag gaccatggtc aagtctgaag
     1921 catcacgcgt tgcctgggtc ttgacgggct caggacacta tctcaaggaa tgcatggaga
     1981 tagtgggcag actgcccgcg gtcgatttgt tcctgagcga ggcggccggt gaggtcctga
     2041 agatgtatgg gtacgatctg gctacgttaa gacgcggcat gaaggtattc cgcgacaaca
     2101 cggccagttc ggtgccggtg acctttttgt acggaggcaa ttaccatacg gtggtgatcg
     2161 gcccggcgag ttccaatacg gtggcgaaat gcgtcgcagg catttccgac acactggcga
     2221 cgaacatcta tgcgcaggcc ggcaaatgcc gaattccgtc catcgtgttt gcatgcgaca
     2281 ccgctccggc cttgatctct gaagcgcccg gtggcgatgt ctgggtttat ccgcgtgcga
     2341 tagatctcga taacgtgaac aggcttgcaa agttcgacca gaccacggtg gtggtctcgg
     2401 tcgaagaact ggggacggca ctggaccaga ggttgagttg tctgagcaca tcctcttcct
     2461 gaccggccgg cttgccgaaa aaaacctgca tcgcgtaatt gaagccatgc agccggcgcc
     2521 cttccagcat tccgtgctgg cgctttccct gcaggttgca ggattgatga cctcggacat
     2581 gatccgccgc aggcttcctg ctctggccgg catcgaccgc gtgatggtgc cgggacgatg
     2641 ccgcggtgac ctcaacgaac tatcttccca tttcggagtt ccattcgagc gtggtcccga
     2701 agacctgaag gacctgccgc gccatttcgg acgtgaagcc gcaccgcggg acttgagccg
     2761 tcatgatgta cgcatctttg cggaaatcgt cgatgccccg aacatgagcc tgacggaaat
     2821 tctcgatcag gcgaatgcat accgaaacgc tggggccgac gtcattgacc tgggatgcct
     2881 gccagaaacg ccgtttccgc aattggaaga tgcaatcggg gctttgcatg catcgggatt
     2941 cccggtcagt gtcgattcaa tggacgccga ggaactgcta cgcggcggac gtgccggtgc
     3001 agattttctg ctgagcctca aaccagaaac cctgtggata gcagaccagg tcgcttcagc
     3061 gccggtactc attgcgtcca cgccgaacga tatgggctcg ctgtatcaag ccatcgaagc
     3121 catggaaaag aaaggccgcg actatctggt cgatccgatt ctggaaccca tccattttgg
     3181 ttttactgaa tcgctggtgc gttaccggga cttgcgcaag cgtttgcccg acgcaaagat
     3241 cctcatgggt accggcaacc tgaccgaact gaccgaagtc gatacgctcg gcgtgcaaac
     3301 gctgttgatg gggatcatct ccgaactgca aataaagaat atcctgacta cacaagtcag
     3361 cgcacattgc gcaggggtta tccgcgaagc cgaccaggcg cgtcgaatca tgcattcagc
     3421 gcgcgacgcc ggcgacttgc cgcaaggctt cggcggagcg ttgctggcgt tacactcgct
     3481 cgatccattt ccctggagtg cgcaggaaat cgcagcgaca gcggccgaaa taaaagaccc
     3541 aagctttcgc gtgcaaatca gcccggacgg catgcatgta tacaaccgcg atggaatgcg
     3601 cacggcgacc gatccgttca agctgtttgc gcacctggca ttggaacagg acggctcgca
     3661 tgctttttac atgggagtgg agctggctcg ggcccagatc gcctggcagc ttggaaaacg
     3721 ttacgtgcag gacagcgaac tcgaatgggg tatcgcgcag ttgcgcgcaa gtagcgatgc
     3781 gcctgttacg ttggttgcga ggccggtaac ctccaaaacg aaagctgttc catgattttc
     3841 gagacgattg tcagcacccg cgatgtggca ggcagtgctc atatcgcacc gatgggcatt
     3901 cgccgtgaag acggattcat tgtcctcagt ccatttcgcc cgtccacgac gctggaacat
     3961 atattgcgcg agcaatgcgc ggtagtgaac tacgtggacg atgtgcggat ctttgcgggt
     4021 tgcctgaccg gacgctatca gtggcccacg cagtccgccg aacgtttgac gtgcccccgg
     4081 ctttccgcgg cgttagcaca ctcggaagtc aatttggagc gcgtgcagga cgatccgaca
     4141 cgcccgcggc tgtattgccg tgagattctg cacgcaaccc atgggccctt ccgcggcttc
     4201 aaccgcgcgc aatcggcggt aatcgaggca gccatcctgg tgagtcgact caacatgctg
     4261 ccgcaggaaa agatcgatgc cgagatggcg tatcttgcca tcggtctgga aaagacggcc
     4321 ggcccgcgcg aactggaagc ctggggctgg ctgaaggaac gaatcgaaca atttcgcagc
     4381 gccagaaagg aaagtgccgc gtgacgatga tgctggccag cgtgcggtcc ctggacgaag
     4441 cattgtccgc gctcgacagc ggcgctgacc tcattgacct caaggaaccg tcgcgtggcg
     4501 cgcttggcgc actcgaccat gccgccgtcc gcatctgtgt ccaggcaatt ggcggccggc
     4561 gaccagtaag cgcgaccatc ggtgatgttc cttccatgga accgagtgca gtgagggacg
     4621 cggtcgaatg catggcagcc accggggtgg atttcatcaa aattggattc ttcttccacc
     4681 cgcacgcgag cgattgcgtg agcgcacttt cacgattggc acgcgaggtc cggttggtcg
     4741 cggttctgtt cgctgacgcg ccttatgatg cagagttgat tgacctgctt gctcgcagag
     4801 ggttttcagg cgcgatgctg gacaccgcac agaaaaacgg caagacgctg cgcgattggc
     4861 ggaccatgcg cgaactcggg aaatttgtaa aacacgtgca ggatctcggg ttactgagcg
     4921 gccttgccgg ctcgctgcgc agtaacgata tagtgcctct gctcgagcta gcgccggatt
     4981 acctcggatt ccgcactgcg ctctgcgcgg attgggacag aaggaaaagc ctcgactgcg
     5041 gggccgcatc ccgcattcgg gcgcttattc ccgctcatca cggccaggaa ttaaccatcc
     5101 gcgaacttcg ggtgtgactt cgcgcctcag gttgccgaag aggtatgttc cctgcgccgg
     5161 tcggccaccc gccgttccgg aaaagaaatc ggcagcacga acgtgcggcg gttgccgtcg
     5221 cgcaattcga tatcgatccc gcgccgcacg ggccgccggg ggcgcaaggt cgtaaggaca
     5281 ctggttggtc cgactgtgaa gttatagatc tcctccatcg gggccgcatc tttgggtcgg
     5341 ctgacttcga aatcccggca cagcatcgca atgaccatct tgatttgaag catcgccagc
     5401 tggcgccctg ggcacaagcg tggtcctgcc ccgaacggaa taaaagcctt cgcgttatgc
     5461 gctcgttcag ggtcgcctga attttgcagc cagcgctctg gacggaattc atttgcatct
     5521 gaaaagttcg cctcctgcac acccaagtga gcgttgaggg cgagaatagg cgtgcctttc
     5581 gggacaaaca cgtcccggat ttgcacgtcc tcgttcggtt ccagaaacgt cacatggcca
     5641 gcgactggct tgcatcgcat ggcttcattc gtgaaagctt cgatgtaccg aagtttgtct
     5701 gccgttgctg gatcctgcca tggctggtcc gccatcccga gaaccgtatc gacttcttcc
     5761 tggacagcag cctggacgtt cgggaactcc atgaggaagt gcatcatgta ggaaagcgta
     5821 ttggcagtgg tatcttcccc agccagcagc atagtcatta cattggcaga gatttcctcg
     5881 tcggtgggcc cttcgtcatc cttttcctgt gcggcgacca aggcttccag a
//
LOCUS       DATMZQ010000119         2930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_738951, whole genome shotgun sequence.
ACCESSION   DATMZQ010000119 DATMZQ010000000
VERSION     DATMZQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738951"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VN496_03790"
     CDS             complement(<1..1495)
                     /locus_tag="VN496_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16098.1"
                     /translation="MCQPKILLAGLLLLSPFSALAERKPVAAQIQLPHNYYYREMYLP
                     QLTSGPSALTFSPDGKELVYSMGGSLWRQTIGSEEAREITHATGYDYQPDWSHDGQHI
                     IFTRYQNDALELWQLNLQTGKEVALTSQGDINLEPRYSPDDRQVAFVSTADSGHFNLY
                     VAEIAGDRLSASKRLLPEHTSKIYRYYYAPTDHAINPFWTPDGKRLLFVSNREVAYGT
                     GDIWSVAVNAPEDLKKIFSEETAWRAQPQVAPDGRRILFSSYHGRQWQQLWLTTMQGA
                     APFPLTFGDFDRTQARFSPDGTRIGYISNEDGNTSLWIQEVLGGARTRITATQRHYLK
                     EVGRLQLSVRDEHGQKVPARVSVIAADGRAYAPDNAWMHTDDGFDRSLQPFENHYFHC
                     PGECTVVLPTGDAQLQVSHGFDYAIAERRVGITKDGNTMTIALKPLRLPAQYGHFASA
                     DLHVHMNYGGHYHNTPLNLIEQARAEHLDAVYNLIVNKEERIPDIAQF"
     gene            complement(1507..2841)
                     /locus_tag="VN496_03795"
     CDS             complement(1507..2841)
                     /locus_tag="VN496_03795"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HXF16099.1"
                     /translation="MNKNPDRRRAIRTELPGPKARAILARDAEVVSPSYPREHPFVMS
                     HGRGVEVWDVDGNRFLDFAAGIAVCSTGHSHPQVVQAIKDAADRFLHISSDYWHEGQV
                     ALAEKINALAPMGEPVQSFFCQSGTEAVEGALKLARYVSGRSRFIGFLGGFHGRTMGS
                     LAFTSSKQTQQQGCATMPGVTHVPFPNTYRPLFAGEDQGQAVLNYIENGLFVSNVPAS
                     EVAAILVEPIQGEGGYLVPPDGFLAGLRQLCNRHGILLIFDEVQSGIGRTGKMFACQH
                     WNVQPDIMTLAKGLGSGLPIGLVVAKKSIMQQWKPGAHANTYGGNPLCCAAALVTLDL
                     IEREYAANAEKVGAYFMDRLRELQSRHQVIGEVRGKGLMIGMELVSDRASRNPCKFSC
                     HTVITRAFHRGLLLLSCGQSTVRFMPPLMVSVAEVDEAMTLLEAALVEAMTQ"
BASE COUNT          616 a          842 c          830 g          642 t
ORIGIN      
        1 tgaactgcgc gatatccgga atgcgttctt ccttattgac gatcaggttg tagacggcgt
       61 ctagatgttc ggcacgcgcc tgttcgatca gatttaacgg agtgttgtga tagtgcccgc
      121 cataattcat gtgcacatgc aggtccgcgc tagcgaagtg gccatattgc gccggtagcc
      181 gcagcggctt gagcgcgatt gtcatcgtat tgccgtcttt cgtgatgccg actcgccgtt
      241 ccgcgatggc gtagtcgaag ccatgagaga cctgcaactg cgcatcgcca gtcggtagga
      301 ccacggtgca ttcacccggg cagtggaagt aatggttttc gaaaggttgc aggctgcgat
      361 caaaaccgtc gtcggtatgc atccaggcgt tgtcgggcgc gtaagcgcga ccatcggcag
      421 caatcacact cactcgggcc ggcacttttt ggccatgctc gtcacgcaca ctcaactgga
      481 ggcgaccgac ctcttttagg taatgccgtt gcgttgcagt gatgcgagtc cgcgcgccgc
      541 cgaggacttc ctgaatccag agcgaggtat tgccgtcctc gttactgata taaccaatcc
      601 gagtgccgtc tggcgaaaag cgcgcttgag tcctatcgaa gtcaccaaag gtcaatggga
      661 acggtgcggc accctgcatg gttgtcagcc acagctgttg ccactggcgt ccgtgataac
      721 tgctgaacaa aatccgccgg ccatctggtg cgacttgcgg ttgcgcgcgc catgccgttt
      781 cttcgctgaa gatttttttc agatcttcgg gcgcgttcac agccactgac cagatatcgc
      841 cggttccata agccacctct cggttcgaca cgaacaacaa gcgcttccca tcgggcgtcc
      901 agaaaggatt gatggcgtgg tcggtgggcg cgtagtagta gcgatagatc ttgctcgtgt
      961 gctcaggtag caaacgtttc gacgcgctca ggcgatcacc agcgatctct gcgacataga
     1021 gattgaaatg gccgctgtca gcggtggaaa cgaaggcgac ctggcggtca tcgggtgaat
     1081 agcgcggttc gagattgata tcgccctgcg aggtcagggc aacttcctta ccagtttgca
     1141 gattgagctg ccacaactcg agcgcgtcat tttggtagcg agtgaagata atgtgttgac
     1201 catcgtgtga ccaatctggt tgataatcgt agccagtcgc gtgagtaatt tcgcgcgcct
     1261 cctcgctccc aatcgtctgg cgccacagcg agccacccat actataaaca agctccttgc
     1321 catcgggcga aaaggttaat gccgatgggc cgctcgttag ctgcggcaga tacatctcgc
     1381 ggtagtaata attgtggggc aactggattt gcgccgctac aggcttgcgc tccgccagcg
     1441 ccgagaaggg agagagtaac aacaggccag ccagcagaat cttgggctgg cacattgtca
     1501 ttcccattat tgagtcattg cttcgaccag ggcagcctct agcaaagtca ttgcctcgtc
     1561 tacctccgcc accgacacca tcagcggcgg catgaagcgc actgtactct gcccacaaga
     1621 gagcaagagc aggcctcgat gaaatgcgcg cgtgatcacg gtatgacacg aaaatttgca
     1681 agggttgcgg ctggctcgat cgctaaccag ctccatgccg atcattagtc ctttgccgcg
     1741 aacttctccg atcacttggt gccgcgactg caattcgcgc aagcggtcca taaaataagc
     1801 gcccactttc tcggcattag ccgcgtactc gcgttcgatc aggtcgagtg tcaccaacgc
     1861 ggcggcgcaa catagcggat ttccaccgta agtgttcgca tgcgcgccag gcttccattg
     1921 ttgcatgatg cttttcttag ctactaccag accgatcggc aggcccgagc ccaggccctt
     1981 ggccagcgtc atgatatccg gctgcacgtt ccagtgctgg caggcaaaca ttttgccggt
     2041 gcgtcctatg cctgattgca cttcatcgaa aatcaacaga atgccgtggc gattgcagag
     2101 ttggcgcaag cccgccagaa atccatcggg cggtaccagg tagccgcctt cgccctgaat
     2161 cggctcgacc aggatggcgg cgacttcgct ggctggcacg ttgctgacga acaagccgtt
     2221 ctctatatag ttaaggacgg cctgaccttg atcctcgccc gcgaacagcg gtcggtaagt
     2281 attcggaaac ggcacgtggg tgacacccgg catggtcgcg caaccttgct gctgggtttg
     2341 cttactggaa gtgaaagcca gcgaacccat cgtgcgcccg tggaaaccac cgagaaagcc
     2401 gatgaatcgc gagcgtccac tgacatagcg cgccagtttc aatgcgcctt ccaccgcttc
     2461 ggtgccggac tggcagaaaa aactctgcac cggctccccc atcggtgcca gtgcattgat
     2521 cttttcggcg agggcgactt gcccttcgtg ccagtaatca gaagaaatgt gcagaaaccg
     2581 atctgcagcg tctttgatcg cttgaaccac ctgcgggtgt gaatggcccg tgctgcagac
     2641 ggcaatgcct gcggcaaaat ccaggaaccg attgccatct acgtcccaaa cttcgacacc
     2701 gcgaccatgc gacatcacaa acgggtgctc gcgcggatac gacggcgaca ccacttcggc
     2761 atctcgcgcc aggatggcgc gcgcttttgg gccaggcaat tccgtccgga tggcgcgcct
     2821 gcgatcggga ttcttgttca tgcgttgacc tggcttgtta acggacgcgg agtttttctg
     2881 gatcgaagca catcaccggc atgagcaatt tccgttcgct tatgcaggtc
//
LOCUS       DATMZQ010000120         5379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2119745, whole genome shotgun sequence.
ACCESSION   DATMZQ010000120 DATMZQ010000000
VERSION     DATMZQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119745"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /gene="thrS"
                     /locus_tag="VN496_03800"
     CDS             <1..1762
                     /gene="thrS"
                     /locus_tag="VN496_03800"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXF16100.1"
                     /translation="RDADLQVITDKDAEGIDILRHSTAHLLAHAVKELYPDAQVTIGP
                     VIEDGFYYDFAYKRPFTPEDLAAIEQRMAEISKRDIRVERQVWDRNQAIEFFRKQGEE
                     YKAQIIGAIPGNETISLYSQGNFIDLCRGPHVPSTGKLKAFKLLKVAGAYWRGDSHNE
                     MLQRIYGTAWAKKEDLDAYLFRLEEAEKRDHRKLGKQLDLFHTQEEAPGMVFWHPKGW
                     AIWQQVEQYMRRTLSHHGYQEVRTPQIMDRSLWERSGHWENYRELMFTTESEKRDYAV
                     KPMNCPGHVQIFNQGLKSYRDLPLRLAEFGACHRNEPSGALHGIMRVRGFTQDDAHIF
                     CTESQVRAEASAFIELLQKVYADFGFTEILIKLSTRPAKRIGSNETWDKAESSLKAAL
                     DAKNLKWDLNPGEGAFYGPKIEFSLKDSIGRVWQCGTLQLDFELPARLEAEYIAEDNS
                     RRLPVMLHRAILGSMERFIGILIEHYAGALPLWLSPEQVVVLNISEHQAQYASQITQQ
                     LKDAGLRSFADLRNEKITYKIREHSLQKLPYQIVVGDKEVAANAVAVRLRSGSDLGQM
                     PLDAFIRRVKSELPHTGSAA"
     gene            1784..2302
                     /gene="infC"
                     /locus_tag="VN496_03805"
     CDS             1784..2302
                     /gene="infC"
                     /locus_tag="VN496_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXF16101.1"
                     /translation="MAQDRDVRINGEITAPEVRLIGMKGEPIGVVSLAQANAMAEELE
                     IDLVEIAPTAQPPVARLMDFGKFKYSEAKKKHEAKLKQKQIQVKEVKFRPGTDAGDYQ
                     IKLRNLTRFLNEGDKTKITLRFRGREMAHQEFGFKLLERVKADLEPYGVVEQFPKMEG
                     RQMVMVMSPKKK"
     gene            2468..2665
                     /gene="rpmI"
                     /locus_tag="VN496_03810"
     CDS             2468..2665
                     /gene="rpmI"
                     /locus_tag="VN496_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXF16102.1"
                     /translation="MPKLKSKSGAAKRFKALGSGRFKRTQANLRHILTKKSTKRKRHL
                     RGNTAVSPSDHGHIRSMLPYA"
     gene            2678..3037
                     /gene="rplT"
                     /locus_tag="VN496_03815"
     CDS             2678..3037
                     /gene="rplT"
                     /locus_tag="VN496_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXF16103.1"
                     /translation="MPRVKRGVTARARHKKVLEKSKGFRGRRGNVYRIAKQAVMRAGQ
                     YSYRDRRRKKRDFRALWIVRINAAAREFGMTYSTLINGLKKAAIEVDRKVLADIAVFD
                     KSAFERIASQAKASLGA"
     gene            3136..4134
                     /gene="pheS"
                     /locus_tag="VN496_03820"
     CDS             3136..4134
                     /gene="pheS"
                     /locus_tag="VN496_03820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXF16104.1"
                     /translation="MDNLDDIIANAKSTFDNAADLAALEQAKARFLGKNGALSEMLKA
                     LSKLPAAERPQAGAMINKSKEHVEALLKARREAIEMVSLEARLSEEALDVTLPGRGRN
                     RGSIHPVIRTWQRVETLFRSMGFDVADGPEIENDWYNFTALNQPENHPARSMHDTFYV
                     EGGLLLRTHTSPMQVRYARRHKPPIKVISPGRTYRVDSDATHSPMFHQVEGLWLDENI
                     SFADLKGVYTDFLRKFFETDALEVRFRPSFFPFTEPSVEIDMAFPAGPHKGRWLEISG
                     AGQVHPSVVRNFGLDPERHIGFAFGSGLERLTMLRYGINDLRLFYEGDLRFLKQFT"
     gene            4145..>5379
                     /gene="pheT"
                     /locus_tag="VN496_03825"
     CDS             4145..>5379
                     /gene="pheT"
                     /locus_tag="VN496_03825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXF16105.1"
                     /translation="MQFPEKWLRELVDPELGSDGLAHLLTMAGLEVEEWRPVSPPFSG
                     VVVGEVLAVENHPGANKLKVCRTNVGASQPVSIVCGAPNVVAGMKAPCALVGAKLPQS
                     ITGQSLEIKVSNVRGVESHGMLCSARELGLSEDHSGLLTLRQDAPVGTNVRDLLDLND
                     HIFTVKLTPNRPDCLSVLGMAREVAALTRAPLRAPDIYAVPKVSEAAFPVKISAPDGC
                     GRFTGRVIRNVNANAPSPEWMKRRLERAGQRPISALVDVTNYVMLELGRPLHVYDLDK
                     LKGAIDVRFGRKSETLKLLNEQTVHLDETVLTITDERGPIGLAGIMGGDTTKADLTTR
                     NIFLEAAFFYPDAIAGRARRYNFTSDASHRFERGVDFNNNIDGIERATALVLEICGGE
                     PGPVQDNVASLPKRRPVTM"
BASE COUNT         1306 a         1397 c         1553 g         1123 t
ORIGIN      
        1 tcgcgacgct gatcttcagg tcatcaccga taaggatgca gaaggcatcg atatcttgcg
       61 gcattcgacc gctcatttgc tggctcacgc agtaaaagag ctttatccgg acgcgcaggt
      121 caccatcggt ccagtgatcg aagacggttt ctattacgat tttgcttaca agcgcccgtt
      181 cacgccagag gatctcgcag caatcgaaca gcgcatggcg gaaatttcca agcgtgatat
      241 cagggtggag cgtcaggtct gggatcgtaa ccaggccatc gaattttttc gcaagcaggg
      301 cgaagaatac aaggcacaga tcatcggtgc gattccggga aacgagacaa tttccctgta
      361 ttcgcaaggc aatttcatcg acctgtgccg cggtccgcac gtgccttcca ccggcaaact
      421 caaggcattc aagttgctca aagtggccgg cgcgtattgg cggggcgatt ctcacaatga
      481 gatgctgcaa cggatttatg gcaccgcgtg ggccaagaag gaagatctgg atgcctatct
      541 tttccgcctt gaagaggcgg aaaagcgcga ccatcgcaag ctgggtaaac aactcgattt
      601 gttccatacc caggaagaag cccccggcat ggtgttctgg catcccaagg gttgggcgat
      661 ctggcagcaa gtcgaacagt acatgcgtcg cacgctttct catcatggct accaggaagt
      721 gcgcaccccg cagattatgg atcgatcgtt gtgggaacga tccggacact gggaaaacta
      781 ccgtgaattg atgttcacga ccgaatcgga aaagcgtgac tacgcggtca agccgatgaa
      841 ttgtcccggg cacgtacaga ttttcaacca gggccttaag agttaccggg atttgccgct
      901 gcgcttggct gagtttggtg cgtgccaccg aaatgaacct tctggcgctt tgcacggaat
      961 catgcgggtg cgcggtttca cgcaagacga tgcccatatt ttctgtactg aaagccaggt
     1021 gcgggctgag gcttcggctt ttattgagct actgcaaaag gtctatgccg attttggatt
     1081 caccgaaata ttgatcaagt tgtcgacgcg cccggccaag cgcattggat ccaatgaaac
     1141 ctgggataag gccgaaagct cgttgaaggc cgcgctcgat gcaaagaacc taaagtggga
     1201 cctgaaccca ggggaaggtg ccttttacgg ccccaaaatt gagttttccc tgaaggattc
     1261 gatcggacgc gtttggcagt gcggaacctt gcagctcgac ttcgaattgc ccgcgcggct
     1321 tgaggccgaa tatattgccg aagacaacag tcgacgcctg ccggtcatgc tgcacagggc
     1381 gatcctgggc tctatggagc gcttcattgg cattctcatt gagcattatg ccggcgccct
     1441 gccactttgg ttatcgcccg agcaggttgt ggtcctcaac atctcggaac atcaggcgca
     1501 atatgccagt cagattaccc agcaactcaa ggatgctggg ttgcgtagct ttgccgactt
     1561 gaggaacgag aaaattacct ataaaatacg agaacatagt ttgcaaaagt tgccttatca
     1621 aatcgtcgtt ggcgataagg aggtagccgc aaacgccgtt gccgtgcgac ttcgcagcgg
     1681 aagcgatctc ggacagatgc cgttggatgc gtttatcagg cgggtgaaat ccgagttgcc
     1741 gcatacgggg agcgcggctt aattttttga tggaggcttg cttatagctc aggataggga
     1801 tgtcaggatc aatggcgaaa taacagcgcc ggaggtccgg cttatcggta tgaaaggtga
     1861 gccaatcggg gtcgtcagtc tcgcccaggc aaatgccatg gcggaagagc tggaaatcga
     1921 tctggtcgaa attgcgccaa cggcacagcc gccagtggcg cggctgatgg actttggcaa
     1981 gttcaagtac agcgaggcca agaaaaaaca tgaggccaag ctcaaacaaa aacagatcca
     2041 ggtaaaggaa gtgaaattcc ggccaggcac ggatgccggg gactaccaga tcaaattacg
     2101 caatctgacc cgttttctga acgaaggcga caagaccaaa attaccctgc gcttccgcgg
     2161 ccgggaaatg gcacaccagg aatttggttt caagcttctc gaacgggtaa aggcggacct
     2221 tgaaccctac ggggtggtcg agcagtttcc aaaaatggaa ggccgccaga tggtcatggt
     2281 gatgagtccg aagaagaagt agtgaagtag caaggttggc tggcaggtgt atccgaacga
     2341 atggcggatt ccgaaacagg acggcaagaa ttcgggccat cagttcgtca aaacaagtga
     2401 ttcccaggca acaaagcgcc ctgcaaggga cgcctggagt caagctaaaa tatgtaagga
     2461 gtaataaatg ccaaaactca agtccaagag cggcgccgcg aagcgcttca aggcgctcgg
     2521 aagcggccgc tttaaacgca cgcaggcaaa cctgcggcac atcctgacca agaaatcgac
     2581 caagcgcaaa cgccatttgc gcggtaacac ggcagtcagt ccgtcggatc atggtcacat
     2641 tcgttcaatg cttccttacg catagcggga gaccaccatg ccaagagtca aacgtggtgt
     2701 aaccgctcgt gcacggcata agaaagttct cgagaagtca aaaggctttc gtggccgccg
     2761 cggcaatgtg taccggatcg ccaaacaagc agtcatgcgt gcgggccaat actcgtatcg
     2821 cgatcgccgc cgcaagaagc gggattttcg cgcattgtgg atcgtgcgca tcaacgccgc
     2881 agccagagaa ttcggcatga cgtacagcac tctgatcaat ggactgaaga aggctgcaat
     2941 agaagtcgac cgcaaggtgc ttgccgacat tgccgtattc gataaatcag ctttcgagcg
     3001 gatcgcgagt caggcaaagg cgagcctcgg cgcctgaagc ggaaagtaaa atgaaaaagg
     3061 aggctggaag gcctcctttt ttttgttcag tcccgttgcg gccaacgggc tcacttggtt
     3121 ctagcatgcg acgagatgga taacctcgac gacatcattg ccaacgcaaa atcgactttc
     3181 gacaatgctg ctgacctggc cgcgctcgag caagccaagg cgcgtttcct tgggaagaat
     3241 ggtgcgctaa gcgaaatgtt gaaagcgttg agcaagcttc cggctgcgga acgaccgcaa
     3301 gccggcgcca tgatcaacaa atcgaaggaa catgttgaag ccttattgaa ggcgcgccgc
     3361 gaagcgatcg agatggtgtc cctcgaagca agactctcgg aagaagcgct tgacgtgacg
     3421 cttcccggcc gtgggcgcaa ccgcggcagt atccatccgg tcatccgcac gtggcagcgg
     3481 gtcgagacgc tattccgctc tatggggttt gacgtagcgg acgggccgga aatagaaaat
     3541 gactggtata actttactgc gctcaatcag cccgaaaatc atccagcccg ctcgatgcac
     3601 gatacgttct atgtcgaagg agggctcctg ctgcgtacgc ataccagccc gatgcaggtc
     3661 cgttacgcca ggagacacaa gccgccaatc aaagtcattt cgccaggtcg aacgtatcgg
     3721 gtcgactcgg acgccactca ttcgccgatg tttcatcaag tggaaggact gtggctggac
     3781 gagaatatca gtttcgctga cctcaaaggc gtttacaccg attttctgcg caagttcttt
     3841 gaaaccgatg cgctggaagt gcgtttccgg ccatcctttt tcccattcac ggaaccgtcc
     3901 gtggaaattg atatggcctt tccggccggc cctcataaag gccggtggct tgaaatttct
     3961 ggagcgggac aggttcatcc cagcgtcgtg cgcaatttcg gcctcgaccc cgagcgtcac
     4021 attggctttg cttttggcag cgggctagag cgactcacca tgctgcgcta tgggataaac
     4081 gacctgcggc tgttctatga gggcgacctg cgctttctga agcagttcac gtaaggcgag
     4141 atccatgcaa tttccggaaa aatggttgcg cgagctggtt gatcccgaac ttggatcaga
     4201 cggtcttgct catctgttaa ccatggcggg actcgaggtg gaagaatggc gacccgtttc
     4261 tccgccattc tcgggtgtag tagtaggtga agttcttgca gtggaaaacc atcccggcgc
     4321 aaacaaactg aaggtgtgcc ggaccaacgt aggagcgtct cagcctgtca gcatcgtatg
     4381 tggtgcacca aacgtcgtgg cgggcatgaa agccccgtgt gcactggtcg gcgcaaagct
     4441 gccgcaatcc attacgggcc aatcgttgga gatcaaggtc tccaatgtgc gcggtgttga
     4501 aagtcacggc atgctgtgct ccgcgaggga actcggcttg tcagaggacc atagcggtct
     4561 gctgacgctg agacaggatg ctcccgtggg gactaacgtg cgcgacctgc ttgacctgaa
     4621 cgaccacata ttcaccgtca agctgacgcc caaccggccc gattgccttt cggtactggg
     4681 catggctcgc gaagtcgcgg cccttactcg tgcaccattg cgcgcccctg atatttacgc
     4741 ggttccaaag gtcagtgaag cggcgtttcc ggtaaagatt tcggcgccgg acggttgtgg
     4801 ccgcttcaca ggacgcgtca tccgtaacgt caatgcaaat gccccgtctc ccgagtggat
     4861 gaaacgacgg ctcgagcggg ccgggcagcg tccaatttct gctttggtcg atgtgacaaa
     4921 ttacgtgatg ctcgaattgg gacggccatt gcacgtttac gaccttgaca aattgaaagg
     4981 tgcaatcgat gtgcgcttcg gcaggaaaag tgaaacattg aaactcctga acgagcaaac
     5041 cgtgcacctt gatgaaacag tgctgacgat caccgacgag cgtggtccga tcgggctggc
     5101 aggaataatg ggcggtgata cgaccaaggc tgatctgacc acaaggaata tatttctcga
     5161 ggcggcgttc ttctacccgg atgccattgc aggacgagcg cgacgctaca acttcacaag
     5221 tgacgcctct caccgcttcg agcgcggtgt cgatttcaat aacaatatcg acgggattga
     5281 gcgggccacg gctttggtcc tggagatctg cggcggtgag ccgggcccgg ttcaggacaa
     5341 cgtggcaagc ttaccgaagc gtcgtcctgt aacaatgag
//
LOCUS       DATMZQ010000121         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1822782, whole genome shotgun sequence.
ACCESSION   DATMZQ010000121 DATMZQ010000000
VERSION     DATMZQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822782"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2325)
                     /locus_tag="VN496_03830"
     CDS             complement(<1..2325)
                     /locus_tag="VN496_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXF16106.1"
                     /translation="MNGSTPTAITPAMGEVRQTARGTHDAACYHCGQSVPRGPELHAV
                     IGGLPRAMCCAGCVAVAEAIVGNGLEDYYRNRDRFPESPREAIPAVVSDLLVFDREEI
                     QRGFVRDIAVNEREAALILDGIHCPACIWLNEAHVARQPGVIAIHVNYTTRRATVRWD
                     TRSIQLSGILAAIQAIGYRAFPYDPGAMETSRKRERRDALLRLAIAGLGMMQIMMYAY
                     PAYVSGEAGVSADALALMHWASLILTCPVVFYSSLPFFRGALRDLKQRRTGMDVPVAL
                     GIGTAFIGSVAATVTQHGAVYFDSIAMFVFFLLGGRYLELHARHRAAAYLEYLGRAMP
                     AVARRFCDAGDTGAIETIPAAALRPGDRVLVRPGENFPADGCIESGDTQADQALLTGE
                     SSPIAKHVGDTVIGGSLNRGNPVTVRVERIGADTVLSGIVRLMERGMQQRPPLQQFAD
                     RIAGQFVALVLAIAAAAALYWMWVDPARALPVAIAVLVVTCPCALSLATPMTMAVATA
                     AMARLGIVVTRGQAIETLARATHFVFDKTGTLTEGRLQLVQVHLLGNADHYRAIALAA
                     ALESRSEHPLGKAIVAAAADQPLAVSGIRNFPGQGIEGRVDGRRLRIGQKKFVAELGP
                     TTFAEESASSATSVWLGDEQGALAQFLLEDRVRDDACELVSDLRAAGHIVMLLSGDGT
                     HAVRAAAASAGIPYQQARMLPDQKLAAVKQMQQDGAVVAMIGDGINDAPVLAQAQVSI
                     AMGSGAPLAQGAADMVLISPRLSDLGRGLRLARKT"
     gene            complement(2366..2572)
                     /locus_tag="VN496_03835"
     CDS             complement(2366..2572)
                     /locus_tag="VN496_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HXF16107.1"
                     /translation="MNENVGSTDRAIRVIAGVAILFSFFLLEGNARWLALIGIVPLAT
                     GLIRWCPAYSVFGFTTGKRTGTPG"
     gene            complement(2703..>3397)
                     /locus_tag="VN496_03840"
     CDS             complement(2703..>3397)
                     /locus_tag="VN496_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HXF16108.1"
                     /translation="VDPAAAWIVGNMLSDPLARSVTFGLDNPLASRYWAAVKTGTSKD
                     MRDNWCVGFTERFTVGVWVGNFDGSPMHDVSGVTGAAPVWAGLVDYLHRRTASMPPQK
                     PHDVLMRRIEYEQDIEAARHEYFIAGTELDVVASKSPGSVRSGIAYPGNGSIIALDPD
                     IPASVQRVRFVAVPQLTGQRWQLDGEPMELTEQSVWWAPQTGQHDLVLVDANGAELDR
                     IHFEVRGGAHSQ"
BASE COUNT          678 a         1101 c         1007 g          611 t
ORIGIN      
        1 cgtcttgcgt gccaatctga gaccgcgccc aaggtccgac agccgcggcg aaatcagcac
       61 catgtcagcc gcgccctgcg ccaacggcgc acccgagccc atggcaatcg agacctgggc
      121 ctgtgccagg accggcgcat cgttaatccc atcgccaatc attgcgacga ccgcaccatc
      181 ctgctgcatt tgcttgactg cggcgagttt ctgatctggc agcatacgcg cctgctggta
      241 cggaattccg gcgctggccg ctgctgcgcg caccgcgtgg gtgccatcgc cggagagcag
      301 catgacgatg tgccccgccg cgcgcaagtc cgacaccagc tcgcacgcat cgtctcgtac
      361 ccgatcttcc agcaggaatt gcgcgagcgc gccctgctcg tctccaagcc atacgctggt
      421 tgcgctggaa gcggactctt ccgcaaaggt tgttggtccc aattctgcga cgaatttttt
      481 ttgcccgatg cgcaagcgcc gcccgtcaac gcggccttcg atcccctgcc ccggaaaatt
      541 tctaattccg gaaacggcca gtggttgatc tgccgcagcc gccacgatcg ctttgccaag
      601 cggatgctcc gaccggctct ccagcgccgc agcaagcgca atggcgcgat agtggtccgc
      661 attgccgagc aagtgcactt ggaccaattg cagccgacct tctgttagcg ttccggtttt
      721 gtcgaacacg aaatgcgtcg ctcgcgcgag tgtttctatg gcctgcccgc gcgtaaccac
      781 tataccgagc cttgccatcg cggctgtcgc taccgccatc gtcatcggtg tggccaggga
      841 gagcgcgcag ggacaggtga ccaccagtac tgctatggca acaggcaagg cacgcgctgg
      901 atcgacccac atccagtaca gtgcggcggc cgccgctatg gccagcacaa gcgctacgaa
      961 ctggcccgcg attctgtcgg cgaactgttg gagcggcggc cgctgctgca tgccgcgctc
     1021 catcaggcgc acgatgccgg atagcaccgt atcggcgcca atgcgctcga cgcgtacggt
     1081 taccgggttc ccgcgattca gagagccgcc gatgacagta tcaccaacat gcttcgcgat
     1141 gggactactc tcgccagtca acagcgcctg gtcggcttgc gtgtcgccac tctcgatgca
     1201 tccgtcagcc gggaaatttt cgccgggcct caccagcaca cgatcgccgg gccgaagtgc
     1261 ggctgccggg atggtctcga ttgccccggt atcgccagca tcgcaaaaac gccgggctac
     1321 tgcaggcatg gcgcggccca gatattcgag gtatgcggca gcacgatggc gcgcatgcaa
     1381 ctcaagatag cggccgccca gcaggaaaaa cacgaacatt gcaattgaat cgaaatacac
     1441 cgcaccgtgc tgcgtaaccg tcgccgccac gctgccaatg aaggccgtgc cgatacccag
     1501 ggcaaccggt acatccatgc cggtccggcg ttgcttcaga tcgcgcaagg ctccgcggaa
     1561 aaatggcaga gaggaataga acacgacagg acaagtcagg ataaggcttg cccagtgcat
     1621 caaggctaat gcatcagcgg aaacgccagc ttcgcctgag acgtacgccg gatacgcata
     1681 catcatgatc tgcatcatcc cgagcccggc gatcgccaat cgcagcaatg catcgcggcg
     1741 ctcgcgcttg cggctggttt ccattgcgcc tgggtcatac ggaaaggcgc gataccctat
     1801 ggcctgtatg gcggcaagaa tccccgaaag ttgaatgctg cgcgtatccc agcgtactgt
     1861 tgcgcgccgg gtcgtgtagt tcacgtgaat ggcgatgacg ccaggctggc gggcgacgtg
     1921 cgcctcattg agccagatgc acgccggaca gtgaatgccg tccagtatca gcgctgcttc
     1981 acgctcgttc acggcgatgt cacgcacaaa tccgcgttgg atttcctctc gatcgaaaac
     2041 gagcaagtcg gaaacgacgg caggaatcgc ctcccgtggc gactccggaa accggtcgcg
     2101 gttgcgatag tagtcttcga gtccattgcc gacgatggct tcggctacgg ccacgcaccc
     2161 cgcgcaacac atggcgcgcg gcaggccgcc gatgactgcg tgcaactccg gcccacgcgg
     2221 cacagactgt ccgcagtggt agcaggctgc atcatgcgtt cctcgcgccg tttgccgcac
     2281 ttcgcccatc gcaggcgtga tggccgttgg cgttgatccg ttcacagctg cgacggccaa
     2341 ttaagggtgg ccgcggcccg ccgcatcagc ccggggtgcc cgtacgcttg ccggtggtga
     2401 atccgaacac gctatacgcc gggcaccaac ggatgaggcc ggtagccagc ggcacgatgc
     2461 cgatcagcgc cagccagcgg gcgttacctt ccagcaggaa gaaggaaaac aggattgcga
     2521 cacccgcaat gacacggatg gcgcggtcgg tactgcctac gttttcgttc atgatcattg
     2581 gctccagata tggtagtcga acctgatcat agcgaccaga cgctgccgga ctcttgaccc
     2641 agatcaaggc ctgggcatgg cgccgagtga cgcggcgatc tcccaagcca agcaggcgtc
     2701 cgctattgcg agtgtgctcc tccgcgcact tcgaaatgga ttcggtccag ctccgcgcca
     2761 ttcgcatcga ccagcacgag gtcgtgctga cccgtttgcg gcgcccacca taccgattgc
     2821 tccgttagct ccatgggttc accatccagt tgccagcgtt gtcccgtcaa ctgaggaacc
     2881 gctacaaagc gcacacgctg cacgctggcc ggtatgtcgg ggtcgagcgc aatgatgctg
     2941 ccattgcccg gataagcgat gcctgaacga actgaacctg gtgatttgga agcgacgaca
     3001 tcgagttcag taccagcgat gaagtattca tgtcgagccg cttcgatgtc ctgctcgtat
     3061 tcgatacggc gcatgaggac gtcatggggt ttctgcggcg gcatggaagc cgttcggcgg
     3121 tgcaggtagt cgaccagccc ggcccacacc ggagccgcac ccgtcacccc ggacacatca
     3181 tgcatcggac tgccgtcgaa gttgccgacc cagacaccga ccgtgaaccg ctccgtaaag
     3241 ccgacacacc agttgtcccg catgtctttg ctggtgccgg tcttgacagc tgcccagtag
     3301 cgcgaagcaa gcggattatc aaggccgaaa gtcacgctgc gggcaagcgg gtcggacagc
     3361 atgttgccaa cgatccacgc agcagccgga tccacga
//
LOCUS       DATMZQ010000122         5877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1131613, whole genome shotgun sequence.
ACCESSION   DATMZQ010000122 DATMZQ010000000
VERSION     DATMZQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131613"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VN496_03845"
     CDS             complement(<1..967)
                     /locus_tag="VN496_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXF16109.1"
                     /translation="MLALRVLKWYLLATIAASSLAAGGLVVGTIAMAEPTSAPAFVEL
                     TPEEKKWLTNHPVIRIGAETNYAPYEFQDSRGHFVGVVADYLEIIRYKLGTRFQVNQL
                     PDFGIVESKLRKKELDVVLAVASSVDREQFLNFTKPYLHYVNVIVTRDNYSFVSGLKD
                     FQENRVAVVEGHSSKQLAARVYPNFNVTAYPDLLEGLMAVSTGKTDGLVDDIFPIVYT
                     IRYRQISNLKIATAVEKALQPQGFSVGVRKDWPELVGILDKVLLSISHEEQREISQKW
                     LSVRYEDKVDYRAIWTSLAVFSAILLAGVFHIRQLSKQRRALLAAR"
     gene            1142..1945
                     /locus_tag="VN496_03850"
     CDS             1142..1945
                     /locus_tag="VN496_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXF16110.1"
                     /translation="MKIRPLSDLHLEHSGRHPPFELPPVTADVVVLAGDIDNGTRAVD
                     WAEHTFPGNTVLYVPGNHEFYGTNLPEAAAALQERTLRSGNVHLLDNKELLVDGVRFL
                     GSTLWTDFALLGVERQEQIFEEARKYVLDFRKIRMGKDFLTPQQTVCLHQHALLFLEA
                     RLQQPFPGKTVVITHHAPHPGSVHPRWTGNLCNPAFVSDLRRLMGKSALWIHGHTHDS
                     FDYCVNGTRVLANPMGYRSSNWRDPQTANKPMMVYTENARFDAGLVIEI"
     gene            complement(2018..2446)
                     /locus_tag="VN496_03855"
     CDS             complement(2018..2446)
                     /locus_tag="VN496_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16111.1"
                     /translation="MTRSFLFVICLALSSLGYAAPAPDTEANRLEAANQLFKLPAYRM
                     VATRQLYESLKTLPAEQHKRALAALSDADVMASLRGIISRSMAQTFTTAELEHLAHFF
                     EAREALTMVNKAQNFQSALDEEVRAASITDPQLIKILIGK"
     gene            complement(2507..2863)
                     /locus_tag="VN496_03860"
     CDS             complement(2507..2863)
                     /locus_tag="VN496_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16112.1"
                     /translation="MHPKTRYVFTASLDVDPAQEILFNELYDSEHVPFLLAVPGVLAV
                     TRVTTEPDASMRIDGQKKALVAEREPKYSAIYELESAEVLLSDAWAEAVDKGRWPKEI
                     RPHIRARRHTLKKVMR"
     gene            3002..3853
                     /locus_tag="VN496_03865"
     CDS             3002..3853
                     /locus_tag="VN496_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXF16113.1"
                     /translation="MKKWASALTAVIVALASSCVLPAERYRIDPDHTFAHFAVVHTGV
                     SSVRGRMGITKGIATFDREKETAEINVDLDPRSIDTGVKKLDAVLSDEMFFNVAKYKT
                     ARYSGRVLAAERYRIDPDHTFAHFAVVHTGVSSVRGRMGITRGSATFDREKETAEINV
                     DLDPRSIDTGVKKLDAVLSDEMFFNVAKYKTAHYSGRAVKFQDGVPWEFEGDLTLHGV
                     TRPVRLIAEHFVCKQVKIMVLDRFVCGGDLQTTLKRSDFGLDKYTSMVSDDVRLTISV
                     EAIREDK"
     gene            complement(3892..4398)
                     /locus_tag="VN496_03870"
     CDS             complement(3892..4398)
                     /locus_tag="VN496_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16114.1"
                     /translation="MSNKVTFFALSFAPMLYPGTTVAAVDLLAQSKVMAVPARAYPEL
                     KQINDRVAVVITDMEANTHQLQEYRKARVRATDRRYSALTREFNRSRSRLSELERKLE
                     KAPALDVNRLSVPAGGDRGSSSSDIRDRSVAAEYRKYEQAKASLKQSIKALSAYYDQQ
                     LREIANIR"
     gene            complement(4514..5470)
                     /locus_tag="VN496_03875"
     CDS             complement(4514..5470)
                     /locus_tag="VN496_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF16115.1"
                     /translation="MANDERRTFMKQAGLAALAAAGGMSLGEIHAAEYRVPFSSGTEA
                     PKLKAPAAATDCHIHFYDDRFPAAAAATLRPPNATIEDYRLFQQRIGTSRTVIVTPST
                     YGTDNRPSLEAAARLGASARVVAVVDDTVTDAELRKLAGQGVCGIRFNLVQKGATSLQ
                     MLEPLAKRVHDLGWHVQIHMLGDQIVQAKDLLGRLPAQIVFDHLGRVPQPAGVQHPSY
                     GTILALVEKGRAWVKVSGAYHDTKVGPPDYADTSAVARAFIAAAPDRMIWGSDWPHPT
                     VKELANKPDDAILFDLISSWVPDANTLHEVLVENPEKLYGFA"
BASE COUNT         1289 a         1654 c         1614 g         1320 t
ORIGIN      
        1 cgcgggcagc cagcagggcc ctgcgctgct tgctgagttg gcgaatgtgg aacacgcccg
       61 ccagcaggat ggcggaaaaa accgccagcg aagtccaaat cgcccggtag tcgactttgt
      121 cttcatagcg aactgacagc cacttctgcg agatttcgcg ctgctcttcg tggctgatgc
      181 tcaacagcac cttgtcaagg attccgacca attccggcca gtccttgcgt acaccgaccg
      241 agaatccctg cggctgcaac gctttttcga cggcagtcgc tattttaaga ttgctgatct
      301 gccggtatct gatcgtgtag acgatcggga aaatatcatc gacaaggccg tcggttttcc
      361 cggttgagac agccatcaga ccttcaagaa gatccgggta agctgtcacg ttgaaattcg
      421 gatacacccg tgccgccaat tgcttcgagg agtgtccctc gacaaccgcg acccggtttt
      481 cctgaaaatc cttgagtccg gatacaaaac tgtaattatc ccgggtaacg atcacattga
      541 cgtaatgcaa atagggtttg gtaaaattca ggaactgttc gcggtctacc gaactggcaa
      601 ccgccagtac gacgtccagc tcttttttcc gcagcttgct ttccacaatg ccgaaatccg
      661 gcagctggtt aacctggaag cgagtaccaa gcttgtagcg gatgatctcg aggtaatcag
      721 ctaccacgcc gacaaaatgg ccccggctat cctgaaattc atagggcgca taatttgtct
      781 ccgcgccgat gcggatcacc gggtggtttg tcagccattt tttctcttct ggcgtgagct
      841 caacaaaagc cggcgcgctg gtgggctcgg ccatcgcaat agtacccacg accaggccgc
      901 ccgccgccag cgaggatgcc gctatagttg caagcaaata ccacttcagt acgcgcaatg
      961 ccaacactga accctcatcg cttcaaaacc gtacccgact gccacagaac gacgcctggg
     1021 agcacgcagc atttacaccg tgatggctct ggcagtgtcc gtcatcagga atgaacgttt
     1081 cctcggattg tcggccaatt tgtgccgctc cggaagcccg gcggcttggc agggcggtgg
     1141 aatgaaaatc cggcctcttt ccgacttgca tcttgagcat tcaggacggc atccgccgtt
     1201 tgaattgccg cccgtcaccg ccgatgtggt cgttctcgct ggcgacatcg acaacggcac
     1261 tcgagccgtt gactgggcag agcacacctt tcccggcaac accgtgctgt atgtgcctgg
     1321 caaccatgaa ttctatggaa caaatcttcc cgaagccgcc gcggcgctgc aggaacgcac
     1381 actacgcagc ggcaacgtgc acctgctgga caacaaagag ctccttgtcg acggtgtgag
     1441 gtttctgggc agcacgcttt ggaccgattt tgcgctcctc ggcgtagagc gccaggagca
     1501 aatattcgag gaagcgcgca aatacgtgct ggacttccgc aagatccgaa tggggaagga
     1561 tttcctgacg ccgcaacaaa cagtttgctt gcatcagcac gcgctgcttt tcctggaagc
     1621 gcggctgcaa caacccttcc ccggcaagac agtggtcata acacatcacg caccgcatcc
     1681 gggcagcgtg catccacgtt ggaccggcaa tctgtgcaat cccgcgtttg tttccgattt
     1741 aaggcggctg atgggcaaat ccgccctgtg gatccatggc catacccatg acagcttcga
     1801 ttactgcgtc aatggcacgc gcgtgcttgc caatccgatg ggttatcgaa gttcgaactg
     1861 gcgggatcca caaacagcaa acaaaccgat gatggtatac acggaaaacg cccggttcga
     1921 tgcaggcctg gtgatcgaaa tctgagttct gcttcctgca gtgtcaggct ggcatcagca
     1981 aggccgttaa tcaagttgca acttcagaca ggtgagctta tttgccgatc agaattttta
     2041 tcagttgcgg gtccgtgatt gaggctgcac gaacttcttc atccagtgcg gactggaagt
     2101 tttgggcctt gttgaccatg gtcagagctt cacgagcctc gaagaaatga gctagatgtt
     2161 ccagttcagc tgtcgtaaag gtttgggcca tgctgcgcga gataatgccg cgcaacgatg
     2221 ccatgacatc tgcatcggac aaggcggcca gcgcgcgttt gtgttgctcg gcgggcagag
     2281 ttttgagcga ttcgtagagt tggcgggttg caaccatccg gtaggccggg agcttgaaca
     2341 attgattcgc cgcttccagc cggtttgcct cggtatcagg cgcgggtgcc gcgtagccca
     2401 gggacgatag ggcaagacaa ataacgaaca gaaaactgcg tgtcatgcta cctccttccg
     2461 atgattggtg agccgtggaa cggtcaatgc agcggccgca gcgggattaa cgcatgacct
     2521 ttttcaacgt atggcgacgg gcgcggatat gcgggcgaat ttcctttggc cagcgtccct
     2581 tgtccaccgc ctcggcccag gcatcgctca gcaaaacttc ggcgctttcc aactcgtaga
     2641 tcgcgctgta tttcggctct cgttcagcaa ccagcgcttt tttctgtccg tctattctca
     2701 tgcttgcatc cggttccgtc gtaacccgtg tgacggccag aacacctggc accgcgagca
     2761 agaacggcac atgctcactg tcgtaaagct cattgaacaa aatttcctgt gccggatcaa
     2821 cgtcaaggct ggcggtaaaa acatatctcg ttttcggatg catcgtattc ctccgtatag
     2881 tgtggtatcg gttcagcaat gttatcagcc accaggcgaa gtgcgcttgc cgcttggccg
     2941 cgcagcagga agtgcgcggc acgcttgcgc cttcgcattt atgaaattca aggagctttt
     3001 gatgaaaaaa tgggcttctg cactgaccgc agtgatcgtt gcgctagcca gcagttgcgt
     3061 tctgccagcc gagcggtacc gcatcgatcc tgaccatacg ttcgctcatt tcgccgtggt
     3121 gcataccggt gtctcgtcgg tacgcggacg catgggtatc acaaagggta tcgccacatt
     3181 tgatagggaa aaggagaccg cggaaattaa cgtggatctc gatccccgat caatcgatac
     3241 tggggtgaaa aaactggacg cggtcttgag cgacgagatg ttctttaatg ttgccaaata
     3301 caaaactgcg cgctacagcg gccgcgttct ggcagccgag cggtaccgca tcgatcctga
     3361 ccatacgttc gctcatttcg ccgtggtgca taccggtgtc tcgtcggtac gcggacgcat
     3421 gggtatcaca aggggtagcg ccacatttga tagggaaaag gagaccgcgg aaattaacgt
     3481 ggatctcgat ccccgctcaa tcgataccgg ggtgaaaaaa ctggacgcgg tcttgagcga
     3541 cgagatgttc tttaatgttg ccaaatacaa aactgcgcac tacagcggcc gcgccgtaaa
     3601 gtttcaggat ggcgttcctt gggaatttga aggcgatctg accttacatg gcgtgaccag
     3661 gccagtgcgt ctgatcgcgg aacatttcgt gtgcaaacaa gtgaaaatta tggtgttgga
     3721 ccgtttcgtc tgtggcggcg atctacagac gacgctgaag cggtcggact tcggactcga
     3781 caagtacact tctatggtaa gcgacgacgt tcgtctaacg atttcggtcg aagcgatccg
     3841 cgaagacaaa taggctacca gccgaacatt cccaggattg acccggacgg ttcatcttat
     3901 gttggcgatt tcccgcaatt gctggtcata gtacgcggac agggctttga tgctctgctt
     3961 aagtgacgcc ttcgcctgct cgtatttccg gtactccgct gcgactgaac ggtcacgaat
     4021 atccgatgat gaactgcctc gatcacctcc cgctggaacg gagagacggt tcacatccag
     4081 cgcgggggcc ttttccagtt tgcgctcgag ttcgctgagt cggctacgcg atcgattgaa
     4141 ctcgcgtgtc aacgcggaat accgcctatc cgtggcgcga acgcgggcct tgcgatattc
     4201 ctgcagctgg tgcgtattcg cttccatatc ggttatgacg acagcgaccc ggtcgttgat
     4261 ctgctttagc tccggatagg ctcgcgccgg gaccgccatg actttggatt gtgccagcaa
     4321 atccacggcc gccaccgttg tcccgggata cagcattggg gcaaacgaca aggcaaaaaa
     4381 cgttactttg ttgctcaaca atcccatact atccggctac ccccgactcg gaaaagtaca
     4441 tcagcatcag aaaatcatgc tgttatgatt cctagctgtt tcaataagca agtttctcgc
     4501 ccaaacggct cattcatgca aacccataca atttttccgg attttcgacc agcacctcat
     4561 gcaaggtgtt tgcgtccggc acccagctgg aaataagatc gaacaggatc gcatcgtccg
     4621 gtttgtttgc cagctctttt accgtcggat gcggccaatc gcttccccag atcatgcggt
     4681 cgggtgcagc cgcgatgaag gctcgggcca ccgcactggt gtccgcgtaa tcgggcggac
     4741 cgacttttgt atcatggtag gcgccagata ccttcaccca cgcgcggcct ttttcgacca
     4801 atgccaggat ggttccatat gacggatgct ggacgccagc cggctgtggc acgcggccca
     4861 gatgatcgaa cacgatctgc gccggcagac gaccgagcag gtctttggct tgcacaatct
     4921 gatcgcccag catatgaatc tgcacatgcc atcccagatc atgcacgcgc ttggccagcg
     4981 gctccaacat ctgtagtgac gtcgcgcctt tctgcacaag attgaaacga ataccgcaca
     5041 cgccctgacc ggcaagtttt cgcagctctg catcagtgac cgtatcatct acaaccgcga
     5101 caacgcgcgc gctagccccg agtctggcag cagcttcgag cgatggacga ttgtccgttc
     5161 cgtacgtcga cggcgtgacg atgacagtcc gagatgtgcc gatgcgctgt tgaaacagcc
     5221 ggtagtcttc gatggttgca ttgggcggtc gcagggtggc agcggctgca gccggaaaac
     5281 gatcgtcgta gaagtggata tgacaatcgg ttgcagcggc aggtgccttc agttttggcg
     5341 cttccgtccc agatgaaaag ggaacccggt actcggccgc atggatttct cccaatgaca
     5401 ttccacccgc tgctgcgagc gctgccaggc ccgcttgttt cataaacgtc cggcgttcat
     5461 cgttcgccat atcagttcct cctgtgtgaa gcggttgatg catcagcatt gtagagccca
     5521 gatccccgga tcaccgcggc ctaagcgtcc gtggggtgca tcgctccggg attgcggaat
     5581 ccagaatgtt aatcctgaac tcacgctgtg ctactcaccg gacaagtcat gcggatgggc
     5641 ataattcaaa aacgcgtgtg cctatcggat catcgcggac ggacttatcc tggcgcggca
     5701 atgccagaca ggttcaggac tgatgggggc ccgataagtc agtcgctgga accaatccaa
     5761 gaggctttgc ctctaatgcg ggatcgcaaa ccactgcctc tcgccaatga caatagatat
     5821 caggcgccgg actttcgttc aggctgcggc ggctgtttca gcgctatcgt tgaccgg
//
LOCUS       DATMZQ010000123         4744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3501978, whole genome shotgun sequence.
ACCESSION   DATMZQ010000123 DATMZQ010000000
VERSION     DATMZQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501978"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..800)
                     /locus_tag="VN496_03880"
     CDS             complement(114..800)
                     /locus_tag="VN496_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF16116.1"
                     /translation="MAHPEQAIKDIVMFFDRLRYYCGVHLVRITGRPLTNFGWSAWRK
                     KTSITYRLLVDRRELLSHCADPALQLSTNFVSAALARGDICVAAFDGGRLVAYEWVAF
                     GPTPHIGGVWVEFDKTARYGYKQFVLPQYRGQRIAAALATHGDGWCKRRGCSRAVSFI
                     DLDNKASWHASLRVGNQTVGYAGYVTIFGMFISFRTFGARQTGFRFHRPVEQAATTFR
                     TSLENERSAS"
     gene            complement(1071..2732)
                     /locus_tag="VN496_03885"
     CDS             complement(1071..2732)
                     /locus_tag="VN496_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF16117.1"
                     /translation="MVRGRILKIGLAAVVVIAVLLGGLYAFIVWEGSSTRPNAPVLET
                     RIAQWLLYHTVPASYRSMKNPLDTAPGSPDVSAGNEIFNNKCALCHAFDGSSKTDIAA
                     GQYPRPPDLRGSDTQRMSDGELFYHIKNGIRHTGMPAWELPDRQLWQVAAYLRNLPKS
                     ALSAEAAREIPVASVASSHYVGSAACKDCHKDIYARWSQTRMANVVQDPHEHPDAITP
                     DLSKPDPLLTFTKDDIAFVYGSKWKQRYFKKVGDDYFVFPSQWDVTHKIWRPYFVKNG
                     TDWWAPLYPPDNFQRPTGPLCDGCHSVNYDITTKKVTEWNVGCERCHGPGSEHVAHQG
                     RPNIINPAKLNYVQANDVCVQCHSQGQPLKNPIQGKYYDWPVGYDVGKKLVDYWKLEE
                     HKLGETSFTHFPEGSAHKNRMQGNDFVQSLMYTRGVTCFNCHDVHGTKNDAILWKPAN
                     KICFDCHGPNTAVGPHAPTIEAHTHHKAGSAGNECVACHMPKIAQTIADVNVRAHTFR
                     FITPVESEALKIPNACNQCHKDKTTAWATDALKSWQDRSPWRVAQ"
     gene            complement(2780..3565)
                     /locus_tag="VN496_03890"
     CDS             complement(2780..3565)
                     /locus_tag="VN496_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16118.1"
                     /translation="MLQSCHHRLSLGSLLSAAALGLGHLPSSLAQGGPPMLTDDPGTP
                     GAGAWEINIAYAQQRTREEKLHSVPFADFNYGLGETIQLKYEVAWLISELPESGGTRS
                     GLDNSLFGVKWRFLDEERNGLDMSVYPQLELENPTGSVSRGIVEPGPNLFLPVEAAHD
                     FGKFKLVGEVGYQIFHSTDNEWVVGVLGATHISESLEVMAEVRSFSQKLLNDGDVVAN
                     VGMRQELNQKVKLVASIGTGLTNHPDSTTFLTYVGVQIVLGEK"
     gene            3910..>4744
                     /locus_tag="VN496_03895"
     CDS             3910..>4744
                     /locus_tag="VN496_03895"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXF16119.1"
                     /translation="MHSLTTDPTAAGLKSSRPAWLLVLLTVQGAQGALRMRLWRTLKA
                     LGTAVLRDGAYLVPNRPALFASVQALEREVTAADGSMQLLELDARDATQQGEFEALFD
                     RTAEYEKVLDEIRQTRRKLKKMAPPAITAAMGRLRRDLESLSVTDFFPGRAAAQATVA
                     LEDLLADANAVLSPDEPHAVEGRIPKLDPASYRGRNWATRKRPWADRLASAWLVRRFV
                     DPQARILWLDSPADCPKDAVGFDFDGATFTHVDGRVTFEVLASSFSLDRDVGVQRIGE
                     VI"
BASE COUNT          992 a         1380 c         1329 g         1043 t
ORIGIN      
        1 agccgtcttc gcctgcgagc aatatagcac cctgtggcag cttgccgaat gcataaatag
       61 tcgctggctt gctgaaatgc accatggaat gctggccggc ttgccgcaga caactacgaa
      121 gccgagcgtt cattctccaa agatgtgcga aatgtcgtag cggcttgctc aactggccgg
      181 tggaaacgga atccagtctg cctcgcaccg aaggtgcgaa acgagatgaa catgccgaag
      241 atcgtgacat acccggcata cccgactgtc tggttaccga ctcgcaagga ggcatgccag
      301 gaagctttgt tatcgagatc gatgaaggaa acggcacggc tgcaaccccg tcgcttgcac
      361 caaccgtcgc catgagtagc gagcgccgcg gcaatgcgct gcccacgata ttgcggaagg
      421 acaaactgtt tgtagccgta tctcgccgtc ttgtcgaact ccacccagac accgccgatg
      481 tgcggcgttg gcccgaatgc cacccactcg tatgccacca agcggccacc gtcgaaggcc
      541 gctacacaga tgtccccgcg agccaacgct gcgctgacga aattcgtgga cagttgcaga
      601 gccgggtccg cgcaatgaga caggagctcg cgcctgtcca ccagcagccg gtatgtaatc
      661 gaggttttct tccgccacgc cgaccaaccg aaatttgtca acggccggcc cgtgatgcgg
      721 acaaggtgta cgccacaata atatcgaaga cggtcgaaga acatgacgat gtccttgata
      781 gcttgttcgg ggtgcgccat gttggtcaca acaacattat ttcgttgtca tccgccccct
      841 tccaaacgga ctatcggccg tttacgggcg cggcgcattg tcgacggata cgattctttc
      901 agatttgttc gtaacgccgc gtctagcaaa gagaagtaag gagaaagcag gcgcgtccat
      961 tgccgcagaa tgcgaccgcc ggaaccagtc gtttcaaaag cgcaccgacc ctgccaattc
     1021 ggcacttttg caacatttcc ggccgtctca gttgttagct gcaacgatgg tcattgggcg
     1081 acccgccatg gcgagcggtc ttgccatgac ttcaacgcat cggtggccca cgccgtggtc
     1141 ttgtccttgt ggcactgatt gcaagcattc gggatcttga gcgcttcgct ttcgacaggc
     1201 gtgatgaacc ggaaagtatg ggcacgcacg ttcacatcgg cgatggtctg cgcaatcttc
     1261 ggcatgtggc aggcgacgca ttcgttgccc gcgctgccgg ccttgtgatg cgtgtgcgct
     1321 tcgatagttg gcgcgtgcgg acccacggcg gtattcggcc cgtggcagtc gaagcagatc
     1381 ttgttggcgg gcttccacag gatggcgtcg ttcttcgtgc catggacgtc gtgacaattg
     1441 aagcaggtca cgccgcgcgt gtacatcagg ctttgcacga agtcattgcc ctgcatgcgg
     1501 ttcttgtgtg cgctgccttc ggggaaatgc gtaaacgacg tttcaccaag cttgtgctct
     1561 tcaagcttcc agtaatccac caattttttg cccacatcgt acccgaccgg ccaatcgtaa
     1621 tattttccct ggatcggatt cttcagcggc tgtccctgag agtggcactg gacacataca
     1681 tcgttggcct gcacgtaatt gagcttagcc ggattgatga tattcggccg gccctgatgc
     1741 gcgacgtgct cactgcccgg tccatggcaa cgctcacagc ccacattcca ttccgtgact
     1801 ttcttggtcg taatatcgta gttcaccgaa tggcaaccgt cgcacagcgg gccggtcggc
     1861 cgttgaaagt tatcgggcgg atacagcggc gcccaccaat cggtaccgtt cttgacaaaa
     1921 tagggccgcc agattttatg cgtaacgtcc cattgcgagg ggaacacgaa ataatcgtcc
     1981 ccgactttct tgaaatagcg ctgtttccat ttgctgccgt aaacgaacgc gatatcgtct
     2041 ttggtgaaag tcagcagggg atcgggcttg gacagatccg gagtgattgc atccggatgc
     2101 tcatgcggat cctgcaccac gttcgccatg cgcgtctgcg accagcgcgc gtaaatatcc
     2161 ttgtggcaat ccttgcaggc agccgatccg acgtagtgcg aagacgccac gctcgcaacc
     2221 ggtatctcgc gtgcggcttc cgctgaaagc gccgacttgg gcagattgcg cagatacgcc
     2281 gcaacctgcc aaagctggcg gtccggaagc tcccacgctg gcatgccggt atggcgtatg
     2341 ccgttcttga tgtgatagaa caattcgccg tcgctcatgc gctgcgtatc tgagccgcgc
     2401 agatcggggg ggcgcggata ctgcccggcc gcgatatctg ttttgctgct gccgtcaaaa
     2461 gcgtggcaca gcgcacattt gttattgaag atctcgttgc cagcagagac atccgggctt
     2521 cccggcgcgg tatccagcgg attcttcatt gatctgtagc tggccggtac cgtatggtag
     2581 agcagccatt gcgcgatacg cgtttccagc acaggggcat ttgggcgcgt gcttgaacct
     2641 tcccagacga tgaaggcata tagtccgcca agcagcaccg caattacaac gaccgcggcc
     2701 aatcctatct tcaaaattct tccgcgaacc atgttcgcct cctcgagatt attctgtgta
     2761 gagcaagtgg cagcgatgcc tatttctctc ccagcactat ttgcacgccc acgtaggtca
     2821 gaaacgtggt ggagtccgga tggttggtaa ggccggtgcc tatggaagca accagcttga
     2881 ccttttgatt caattcctgg cgcataccca cattggccac gacatcaccg tcattcagaa
     2941 gcttctggct gaagcttcgc acttccgcca tgacttccag cgattcagaa atatgcgttg
     3001 cacccagtac accgacgacc cactcgttgt cggtggaatg gaatatttga taaccgactt
     3061 cgccgaccaa tttgaatttg ccgaagtcat gcgccgcttc caccggaaga aacaggttag
     3121 gccccggttc cacaattccg cgcgagaccg acccggtagg attttccaat tcgagctgag
     3181 gatagaccga catgtcgagg ccgttgcgct cctcatccag aaatcgccac ttcacaccaa
     3241 acaacgaatt gtccaggcca cttctagtgc cgccactttc cgggagctcg gagatcagcc
     3301 aggccacctc atacttgagc tgaatggttt cgcccaggcc atagttgaag tccgcgaatg
     3361 gaacgctgtg cagtttctcc tcgcgcgtgc gctgctgcgc ataggcaata ttaatctccc
     3421 acgccccggc tcccggcgtg ccgggatcat cagtaagcat tggcgggccg ccctgtgcca
     3481 gcgacgacgg caagtgaccc aagccaagcg ccgctgccga caacagtgaa ccgagcgaca
     3541 agcggtggtg gcaggattgc agcacggtcg ccttttactg gttacccgaa gcgaaatcat
     3601 cgaaagtcgt cacgctatta gcctttcgca gactcgaccg ggccgatcct gattcgtatc
     3661 ggtatgctgc cggtcgacga ccggccacgg caaatctggt ttggattgca tgaattccgt
     3721 caatttgcca aatactttgc ccacggcgtt cggcgaagct ctctcgccgt ccatgcttct
     3781 aacttgcatg aacatgacgc gcggcgattg ccagagaaaa cggttatgca gtgattgacc
     3841 ccgcgaaaag ttcgttcaat gtcgggcgga tatgttactt atacaacttg tttgttatct
     3901 ttgctaccaa tgcattcact gacaaccgat ccgaccgcgg ctgggctgaa gtcttcgcga
     3961 ccggcctggc tgcttgtatt gcttacggtg caaggcgccc aaggcgcctt acgcatgcgt
     4021 ctttggcgaa cgctgaaggc gctcggcacc gcagttctca gggatggcgc ttacctggtg
     4081 ccgaatcggc cggccttgtt tgcatcggtg caggcactcg agcgtgaggt gaccgccgcc
     4141 gacggaagta tgcaattgct ggagcttgat gctcgtgatg ctacacagca gggtgaattc
     4201 gaagcgttgt tcgatcgcac ggcggaatac gaaaaagttt tggacgagat ccgacagacg
     4261 cgcaggaagc tcaaaaaaat ggcgccgcca gcgatcaccg cggcaatggg ccggttgcgc
     4321 cgcgaccttg agagcctttc cgtaacggat ttcttcccgg gacgcgctgc cgctcaggcc
     4381 acagtggcgc tggaagattt gcttgccgat gccaatgcgg ttctttcgcc cgatgaaccg
     4441 catgcggtag agggacgcat tcccaaactg gatcctgcct cttaccgtgg ccgcaactgg
     4501 gccacgcgca aacgtccgtg ggccgatcgc ctcgcgagtg cctggctggt ccgcagattc
     4561 gttgatccgc aagcgcgcat tctgtggcta gatagccctg cagactgccc aaaggatgcg
     4621 gtcggcttcg actttgacgg cgcaacgttc acgcacgtag acggtcgtgt gacgttcgaa
     4681 gtgctcgcat caagtttttc tctcgatcgc gatgtcggcg tacaaaggat cggcgaagtg
     4741 attc
//
LOCUS       DATMZQ010000124         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2859619, whole genome shotgun sequence.
ACCESSION   DATMZQ010000124 DATMZQ010000000
VERSION     DATMZQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2859619"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..214
                     /locus_tag="VN496_03900"
     CDS             32..214
                     /locus_tag="VN496_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16120.1"
                     /translation="MPQYWQFIKLKSGDWLWEIRDPNGIAYGVGPFRTFDACVQDAKE
                     KGFEQKNRDRRKDRRQ"
     gene            complement(310..1233)
                     /locus_tag="VN496_03905"
     CDS             complement(310..1233)
                     /locus_tag="VN496_03905"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXF16121.1"
                     /translation="MAKVPAVAPADCHAQLNAALDDALEEFAKKPAISDEGVHAARKA
                     LKRARAGLRLLRNVLDDALYRSKNATLRDTGRQISAMRDSRSLIIAFDSMRDVVPRLR
                     QVRFRKIRSTLLAKHNHAREEFARKAEEQQRAIELLAECSAEIRKWILPSNDPDAIGN
                     GLQKIYKRGLKSYKFARDLVTAEAIHEWRKQVKYLANALGMMAKGGNKKDIKYKKDLD
                     KLADKLGDDHDLVMLARELAGPSPIDAALRVELISLVTQRRSTLKARAFAMGKDLYDL
                     KPKQWTARYVAQGESGQPVRNQQGLPASAHP"
     gene            1433..1816
                     /gene="crcB"
                     /locus_tag="VN496_03910"
     CDS             1433..1816
                     /gene="crcB"
                     /locus_tag="VN496_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXF16122.1"
                     /translation="MGGAGFVAVGIGASLGAWLRWWLGIALNSINPNLPYGTLAANLL
                     GGYLVGVAVQYFSQQTALPIELRLLVITGFLGGLTTFSSFSAEAVGLLMESRYGWAAA
                     LIVSHLAGSILMTILGIVSVRAVTG"
     gene            complement(1806..>2771)
                     /locus_tag="VN496_03915"
     CDS             complement(1806..>2771)
                     /locus_tag="VN496_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXF16123.1"
                     /translation="TVTFLGLKPGLMTLDGPDCAGEISLNTLKVDVPDIPPPRGWRIG
                     PQVIPQLLPPRRRNSHKGTFGNVGLIGGAPGMVGAAILAGRAALKLGAGRVFVGLLGD
                     VPSVDFAQPELMLRTAQDVLSMEALGCLAVGPGLGESQAAQHALTAALSHRAPLVLDA
                     DALNLLASHPQLQRAIAERSSASIITPHPAEAARLLAMATQQIQNDRIEAARMLSERF
                     AASVVLKGNGSICAFESGEWFVNTSGNPGMAAAGMGDVLTGMVASLIAQGGAPGLALL
                     AAVHLHGLAADELVSGGIGPVGLTASEVIDAARKILNQTRTTVSR"
BASE COUNT          573 a          794 c          775 g          629 t
ORIGIN      
        1 gaacaccgag cccgcacact aaagaacgac gatgccccag tattggcagt tcatcaagct
       61 caaaagcggc gactggctgt gggaaattcg cgaccccaac ggcatagcgt atggcgtagg
      121 tccgttcagg acttttgacg cttgcgtgca agatgccaaa gaaaaaggat tcgaacagaa
      181 gaaccgggat agaaggaaag accggcgcca gtgaatttcg cgaccattgt ggccatcaag
      241 aaagaaaaat aactggctga aaatctgcct ttttcaagtg ctgcgcctgc ctcattgaag
      301 aatccctgac taagggtgcg ccgaagccgg aagcccctgt tggttccgta ccggctgtcc
      361 actttcaccc tgtgcgacgt atcgtgcagt ccactgcttt ggttttaagt cgtagaggtc
      421 cttgcccatt gcaaaagcgc gtgctttcaa tgtggaccgg cgctgcgtta ccaatgaaat
      481 caattccact cgtagcgccg catctatggg cgatggcccg gccagttccc gggccagcat
      541 gaccagatcg tggtcatcac ccagcttgtc ggccagtttg tcgaggtctt ttttatattt
      601 gatatccttc ttgttacctc cctttgccat cataccgagc gcattggcca gatacttcac
      661 ctgcttgcgc cattcatgga tcgcctcggc ggtaaccagg tcacgcgcaa acttataaga
      721 cttaagtccg cgcttgtata tcttttgcaa tccattgcca atcgcgtcgg gatcattgct
      781 gggtaaaatc cactttctaa tctcggcact gcattcggcc aacaattcga tggcacgctg
      841 ttgctcctcc gctttgcggg caaattcttc tcgagcgtga ttgtgttttg caagcaacgt
      901 tgatctgatc tttcggaatc gcacctgcct taggcgaggc accacgtccc gcatcgagtc
      961 aaaggcgatt attaaagacc ttgagtcccg catagccgaa atctgcctgc cggtatcgcg
     1021 caaggtcgcg ttcttgctac ggtataaagc gtcatccaac acgttccgca gcaaccgcaa
     1081 accggcccgc gcacgcttca acgcttttct ggccgcatgc actccttcat ccgatatcgc
     1141 cggtttcttc gcaaattctt cgagggcgtc gtcgagcgct gcattcaatt gtgcatggca
     1201 gtcggctggc gccacggcag gaactttggc catctcaatt tctcgggaca caagcggata
     1261 tgaaacgtcg agttgcaaat tgttcccccc agttttgacc caggcgtttg caaaacggtc
     1321 ggtgaccaat cgagacgtgt cccagggagg actctgtagc gttgatctac gtccagcttg
     1381 gacagttatc ggtttattat ctgccgtttc gcataatcag gcgaggtggc agatgggcgg
     1441 tgcgggattc gtagctgtcg ggatcggggc gtctctgggc gcatggttgc gctggtggct
     1501 cgggattgca ctgaacagca ttaatccgaa cctgccatac ggcacgcttg ccgcaaatct
     1561 tctcggcggc tatctggttg gtgtcgcggt ccaatatttt tcgcaacaga ccgcgctgcc
     1621 gattgagctg cgcttgctgg tcattaccgg ttttctcggg ggactaacca cgttctcgtc
     1681 tttctcggcg gaagcggttg gattgctgat ggaaagccga tacggctggg cagccgcgct
     1741 gatcgtaagc catctggccg gctcgatcct tatgacgatt cttggcatcg tttccgtgcg
     1801 tgcggtcacc ggctgacggt agtacgcgtc tggttcagga ttttgcgtgc ggcgtcgata
     1861 acttcgcttg cggtcaggcc aacggggccg attccgccag aaaccagttc atcagcagcg
     1921 agtccatgca gatgtaccgc agcaagcaat gccagcccgg gagcaccgcc ttgagcaata
     1981 agcgacgcaa ccatcccggt caacacgtca cccattcccg ccgctgccat gccgggattg
     2041 ccgctggtgt tcacaaacca ttcgccactt tcgaatgcgc aaatgctacc gttgcctttc
     2101 agtacgacgg aagcggcaaa ccgttcggaa agcatgcgag cagcttctat acggtcattt
     2161 tgaatttgtt gcgttgccat tgccagcaag cgggccgcct cggccggatg cggagtaatg
     2221 atggaggcgc tggagcgttc ggcgattgct cgctgcagct gaggatgcga tgccaacaaa
     2281 tttagcgcat cggcgtcgag caccagcggc gcccgatgcg acagcgcggc ggtaagcgcg
     2341 tgctgggctg cctgggattc gcccaatccc ggcccgacgg caaggcagcc cagggcttcc
     2401 atcgacagta catcctgcgc agtgcgcagc atgagttcgg gttgagcgaa atcgactgac
     2461 ggaacatcgc ccagcaatcc gacgaacaca cgacccgcac cgagtttcag tgctgcgcgg
     2521 cccgccagaa ttgcagcgcc caccatgccg ggcgcacccc ctatgagacc gacattgcca
     2581 aaggttcctt tgtgactgtt gcgccgtcgc ggtggcagca actgcgggat aacttgcggg
     2641 ccgatgcgcc agccacgcgg gggcgggatg tctgggacat cgactttaag cgtgttgagg
     2701 ctgatttcgc cggcgcagtc ggggccatcg agagtcatca gacctggctt caatcccaaa
     2761 aaagtcacgg t
//
LOCUS       DATMZQ010000125         6992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1132530, whole genome shotgun sequence.
ACCESSION   DATMZQ010000125 DATMZQ010000000
VERSION     DATMZQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132530"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VN496_03920"
     CDS             <1..814
                     /locus_tag="VN496_03920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF16124.1"
                     /translation="LYAPLLRRHTSLVFGSHMQQQQWCARYRLPAAKSRVIYNGVDQS
                     HFNVAAVPRTKEQLRRDYGFPADAFIAINVGQLRPEKQQTDLIRAVAVLASRRQAVHA
                     LVVGGGVEHGTIERCIAEFGVGHSARLMGATGDVRGLLKAADAFVLTSTAVETFSNAA
                     LEAMAMGLPVILSRTGGAAEMVDAGKNGFLFSPGNVDELAGHIATLAGDKGMTRAMGE
                     AAATRVRDSFSFMNMLEEYRSLLFPRANRLMPVVEKESFPAAYRHLTGSEAD"
     gene            complement(811..1854)
                     /locus_tag="VN496_03925"
     CDS             complement(811..1854)
                     /locus_tag="VN496_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HXF16125.1"
                     /translation="MDTTATVVVTGAAGFIGFHVSRRLMARGHKVIGLDNLNDYYDVR
                     LKLDRLEQLGECKQFSFVAAGVEDREALQQVFAHAGAVRVVHLAAQAGVRYSIDHPET
                     YVTSNLVGFANILEACRHSKAGHLVYASTSSVYGASRMIPFSEHAVTDHPVSFYAATK
                     KANEAMAHSYAYLYGLPCTGLRFFTVYGPWGRPDMALFKFTQAILAGEPIPVFNNGNM
                     VRDFTYVDDIVEAVIKALDRPATPDPDWNGKAPDPATSSAPYRIYNIGNSKPEKLLRY
                     IEMLEQCLGRRAIMDFQPMQPGDVLETYADTSELDKAVGYKPDTPIEVGVKRFVDWYL
                     EYRARKYQLEPSL"
     gene            complement(1923..3917)
                     /gene="asnB"
                     /locus_tag="VN496_03930"
     CDS             complement(1923..3917)
                     /gene="asnB"
                     /locus_tag="VN496_03930"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXF16126.1"
                     /translation="MCGFAGVVGYRAGNACDWASSLHSMGDAISHRGPDDSGIWTDAD
                     AGIGLVHRRLSIVDLSRAGHQPMVSASSRFVFVFNGEIYNHLELRKALASNYVCPARA
                     GNIQWRGHSDTETLLAGFEVWGVEATLKKAVGMFAFALWDRSERMLYLARDRMGEKPL
                     YYGWQKGILLFGSELKALKKHPSFHADIDRNALMLLLRHGYITAPYSIYKSIRKLPAG
                     TYLKVAVNDRRNDAVAPPEPASYWSLASTIESGRKNLFRGTDTEAVSALELRLEESVS
                     RQMMADVPLGAFLSGGVDSSTIVAMMQVQSSRPVRTFTIGFQEEGYNEAQYAGRVARH
                     LGTDHTELYVTAEEARKVIPRLPTLYDEPFSDSSQIPTFLVSHMARQHVTVALSGDGG
                     DELFGGYPRYLQMTNIWRYVGKLPPVVRNALAAALTSLSPDAWNRMYRPLRQMLPRRL
                     GSFPLGNKVFTLAQILACKHPEDIYYGLLSHWKNPAQVVIGAREPGTVLTHRRQSNWN
                     ADIEHYMMYVDSMSYLPDDILVKVDRAAMGASLETRVPFLDHRVVEFAWRLPLSMRIR
                     NGQGKWILRQVLDKHVPRALIDRPKMGFGVPIDSWLRGPLRDWGEALLNEQRLKREGF
                     FDPAPIRQKWHQHVAGQCNWQSHLWNVLMFQAWLEIEQSQ"
     gene            4265..5419
                     /locus_tag="VN496_03935"
     CDS             4265..5419
                     /locus_tag="VN496_03935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXF16127.1"
                     /translation="MKVCLVGLDNLPVLAPEYRALAMGGETVQQTLLALALARHGHEV
                     SMVVADQGQEDGARWHSIRVFKAFPLNAGIPVLRFIHPRWTGMWSALQRADADVYYTS
                     CAGMQVGLIALFCWRHRRRFVFRTASDTDCDPSRVLLKHARDRWLYEQGLRRADAILV
                     QSVSQANNMAQNYGLQSRVAGMLVEAPPVSAAVRDIELLWVSNIQHLKRPDRLLRIAD
                     CLPNVQIHMVGGPMPGQESIYTQIRDAAATRTNVVFHGRLSYWDTNALYDRAKLLVNT
                     SDVEGFPNSYLQSWIRGVPVVTLLDPDRVIERNGLGAVARSPAELAGLVQSLLLDPAA
                     RQAASDRCRRFMAQEFGEEKIVSVYLETFQNLLQRRLDARVIPSSESPHA"
     gene            5412..6521
                     /locus_tag="VN496_03940"
     CDS             5412..6521
                     /locus_tag="VN496_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HXF16128.1"
                     /translation="MPNTPFIITVDTEGDNLWERPREIGTRNAEFLPRFQMLCERFGF
                     KPVYLTNYEMAMSDAYVEFARDVLCRDACEIGMHLHAWNSPPLQALTEDDFFHQPYLI
                     EYPDSVMREKIHVMTALLEDKFDRKMISHRAGRWGFDERYAAMLLQEGYQVDCSVTPG
                     VNWGANRGNPNGNGGSNYEAFPDHPYFLDPANISAPAPYGLLEVPMTIVLSSLYRKAS
                     YLYRVPLLRRAANRLSPGLSWLCPVQPSLGAPLARSMDVMLQMARATRNEGVACMEFM
                     LHSSELMPGGSPNFATPSDIDQLYDRLEMLFEELSTWCYGLTLHEFRDLWIQLCGHTG
                     MRAFAYQQPDQTGALTATAQFNARSIPVGKQAIPE"
     gene            6518..>6992
                     /locus_tag="VN496_03945"
     CDS             6518..>6992
                     /locus_tag="VN496_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16129.1"
                     /translation="MIRLEGRAALYGDVWFEEELPADAGVDIIRYHCRPKPVAGARSF
                     EFLSMTTNLLIDPEAIGDAFSKDCRYKIRRADTKDGLVMECITSPEPRLAEFCAFFDE
                     FARQKSHDPSDRQWLHAACKAGQLVLTSASRSGEVLVWHAYLLCGAATWLQYSGSC"
BASE COUNT         1516 a         1982 c         1961 g         1533 t
ORIGIN      
        1 cttgtatgcg cctttattac gccggcatac gtctttggtg tttgggagcc acatgcaaca
       61 gcaacagtgg tgtgcgcgtt accgcctgcc tgcagccaaa agccgggtca tttacaacgg
      121 cgttgaccag tcccatttca atgtcgcagc ggtgcctcga accaaggagc agctgcgcag
      181 ggactatgga tttcccgcgg acgcattcat tgccatcaac gtcggtcagc ttaggcccga
      241 aaagcaacaa acggatttga tccgggccgt ggcagtcctg gcctcgcgtc ggcaggcggt
      301 tcatgcgctg gtggtagggg gcggagtcga gcatggcacg attgaacgct gcattgccga
      361 attcggagta ggccacagcg ctcgtttgat gggagccacc ggcgacgtgc gcgggctcct
      421 gaaggcggca gatgcatttg tgctcacgtc cactgcggtg gaaacgtttt caaatgcagc
      481 gctcgaggcg atggcgatgg gtctgcctgt tattctatcg aggaccggag gtgcagctga
      541 aatggtggac gccggcaaga acggtttcct gttttcgccc ggcaatgttg acgaactggc
      601 cggccatatt gccactctgg caggtgataa agggatgact agagcaatgg gggaggcggc
      661 agccacccgg gtccgagact ctttcagctt tatgaacatg cttgaggaat accggtcgct
      721 gttatttcct cgcgcaaacc gactcatgcc cgttgtcgaa aaggaaagct ttcccgcagc
      781 atatcggcac ctcactgggt cggaggccga ttagagcgac ggttcaagtt ggtattttcg
      841 tgctcggtat tccagatacc agtcgacgaa ccgtttcacc cccacctcaa tcggtgtatc
      901 gggcttgtag ccaacggctt tatccagctc ggaagtatct gcgtatgttt ccaggacgtc
      961 acccggctgc atcggctgga agtccatgat tgcccttctg cccagacatt gttccagcat
     1021 ttcgatgtaa cgcagaagtt tctctggctt gctgttgcct atgttgtaga tccggtaggg
     1081 cgcgctgctg gtggcaggat ccggtgcctt gccgttccag tccggatccg gcgtagcagg
     1141 ccgatcgagc gctttgatga cagcctccac aatatcgtcc acataagtga agtctcggac
     1201 catgttgccg ttgttgaaaa ccgggattgg ttctccagca agaattgcct gcgtgaactt
     1261 gaacagcgcc atgtccggtc tcccccaggg accgtacacg gtgaaaaacc gtaagcctgt
     1321 gcagggcaac ccgtaaaggt aagcgtagct atgcgccatg gcttcgttag ccttcttcgt
     1381 agccgcgtag aacgataccg ggtgatccgt aacagcatgc tcggagaatg ggatcatccg
     1441 gctggcgccg tacactgagc tggtcgaggc atacacgaga tggccggctt tactatgccg
     1501 gcaagcttcc aggatattcg cgaacccaac gagattgctc gttacgtagg tctccggatg
     1561 atcgattgaa taacgcacgc ccgcctgagc ggcaaggtgc accacccgca cagcgccggc
     1621 gtgcgcgaac acctgttgca acgcctcgcg atcttcgacg cctgcggcta caaatgaaaa
     1681 ctgcttgcat tcacccagct gctcaagccg gtctagttta aggcgcacat cgtagtagtc
     1741 attcaggttg tccaacccga taaccttgtg tccgcgcgcc atgagccgcc gcgacacatg
     1801 aaagccgata aacccggctg cgccggtcac aacaaccgtc gcggtggtat ccaaaatagt
     1861 ttccctcttt gttctcgata tcaggtccac gggataggca tgtcaacgtt ttgcttgccg
     1921 cgtcactgtg actgttcgat ttccagccac gcctggaaca tcaacacatt ccagaggtgg
     1981 gactgccagt tgcattggcc tgccacgtgc tggtgccact tttggcgaat cggcgcggga
     2041 tcgaaaaacc cctctctttt gaggcgctgc tcgttcagta atgcttctcc ccaatcgcgc
     2101 agtggaccgc gcaaccagga gtcaatcggc acgccgaatc ccatcttggg cctgtcaatg
     2161 agcgctctag gcacatgctt gtccagaact tgccgcaaga tccattttcc ctgaccgttt
     2221 cgaatgcgca tcgatagagg caagcgccac gcaaactcca ccacccggtg gtcgaggaac
     2281 ggcactcgcg tctccagact cgcgcccatg gcagcgcgat ccactttcac aagaatatcg
     2341 tcaggcaggt agctcatgga atccacatac atcatgtagt gctcgatatc cgcgttccag
     2401 ttggattgtc ggcgatgcgt tagtaccgta cccggctcgc gcgcgccgat gacgacctgc
     2461 gctggatttt tccaatgaga aagcagtccg taataaatgt cctccggatg tttacacgca
     2521 aggatttggg ccagcgtaaa aaccttgttg cccagcggaa atgagcccag gcgccggggc
     2581 aacatctggc gcagggggcg atacatccgg ttccaggcat cgggtgaaag cgacgtgagt
     2641 gccgcagcca gtgcattgcg tacgaccgga ggcagcttgc caacgtagcg ccatatgttg
     2701 gtcatctgaa gatatcgcgg atacccaccg aacaactcat cgccgccatc tcccgaaagc
     2761 gcaacggtca catgctgccg agccatgtgg gaaaccagga aagtgggtat ctgtgaagaa
     2821 tccgaaaacg gttcgtcgta aagcgtaggc aaccgcggaa tcactttccg ggcctcctca
     2881 gcagtgacgt aaagctcggt atggtcggtc cccaaatgcc tggcaactct gccggcgtac
     2941 tgtgcttcgt tataaccttc ttcctgaaaa ccgatagtga acgttctgac cgggcggctt
     3001 gattgcactt gcatcatggc gacgatggtg gaagagtcca ctccgcccga caggaaggca
     3061 cccaatggca catcggccat catttgacga ctcactgact cttccagcct gagttccaga
     3121 gcgcttactg cctcggtatc ggtaccacgg aaaagatttt tgcgcccgct ttcgatcgtg
     3181 ctggccaggg accaatagga ggccggctct ggcggcgcaa ctgcatcgtt gcgccggtca
     3241 tttacggcga ccttgagata tgtccccgca ggcagcttgc gaatggattt atagattgag
     3301 tatggcgcag taatgtagcc gtgccgtaac aacaacatga gcgcattacg gtcaatatcg
     3361 gcgtgaaacg atggatgctt tttcagcgcc ttcagctcgg aaccaaaaag caggattccc
     3421 ttttgccagc cgtaatacaa aggtttctcg cccatgcgat cgcgggcgag atacagcatg
     3481 cgttcgctac gatcccacaa tgcgaaagca aacatcccga cagccttctt aagagttgct
     3541 tcgacacccc agacttcaaa accggcaagc aaggtttcag tatccgaatg cccgcgccac
     3601 tggatatttc ccgctcgggc cgggcaaacg taattgcttg ccaatgcttt ccttagctcc
     3661 agatggttgt aaatctcccc gttgaaaaca aatacaaagc gactgctggc agacaccatg
     3721 ggttgatgtc ccgctcgcga caagtccacg attgataggc gccggtgcac aagaccaata
     3781 ccggcatcgg catccgtcca gatacctgaa tcgtctggac cacgatgcga gatggcatcg
     3841 cccatgctgt gcaatgacga tgcccaatca caagcattgc cggccctgta cccgaccaca
     3901 cccgcaaagc cacacattgc ttgagctacg cctttatgcc gaatgaaacg tcgaatcctt
     3961 accagcagaa cttcctgtgt ccatgcgcgt ctagctttat ctaacggcac aatgagacaa
     4021 gtttttcgcc ggatagggac agcgaagcga ttctacaagt cagtctctaa acgacgccgg
     4081 attgcggtat agcggaatgt gggccgattg gactagcggc tatcccggcg tctgcgcgac
     4141 gttgttttat acaattcagt agtcctgcac caccaaaagg aagtctgatg ttcagtagtg
     4201 caacttcaag cgacgctgtt tattgcaatg attgcaatgg gcaaggcctg gcgtggcgat
     4261 gcaaatgaaa gtatgtctgg tcggtttgga taacctgccg gttctggcac ctgaatacag
     4321 agcgctcgcc atgggcggcg agacggtcca gcaaacgttg ctcgcgctcg cgctggcacg
     4381 ccacggccac gaagtaagca tggtggtcgc ggaccaaggc caggaagatg gcgcgcgctg
     4441 gcattcaata cgggtattca aggcttttcc tctgaatgcg ggtatacccg tgctgcgatt
     4501 catccatccg cgctggaccg gaatgtggtc agcattgcaa cgggccgacg ccgacgtgta
     4561 ttacaccagc tgcgcgggca tgcaggttgg gctgatagcg ctgttctgct ggcgccatcg
     4621 tcgacgcttc gtattccgca ctgccagcga tacggactgc gacccctcgc gcgtgctcct
     4681 gaagcatgcg cgtgatcgct ggttgtatga gcaaggccta cggcgagccg acgcgatcct
     4741 ggtacagagt gtttcgcagg caaacaacat ggcacagaat tacggtctgc aaagtcgggt
     4801 ggcgggaatg ctagtggaag ctcctcccgt ttccgctgct gtgcgcgaca tcgaactgct
     4861 atgggtcagc aacatccagc atttgaaaag accggatcgc cttctgagaa tagccgactg
     4921 tcttccgaac gtgcagattc atatggtcgg gggacccatg cctggccagg aatcgattta
     4981 cacgcagatc cgagacgcgg cggcgactcg gaccaatgtg gtgtttcacg gccgcctgtc
     5041 gtactgggat accaatgcgc tttatgaccg ggccaagctg cttgtcaaca cgtccgatgt
     5101 ggaaggattt cccaattcgt atcttcaatc ctggataagg ggcgtacccg tggtcacact
     5161 gctcgacccg gatcgtgtga tcgagcgcaa cggattgggg gccgtggcgc gctcaccggc
     5221 tgaactggcc ggtcttgtcc aatccctgct gcttgatccg gctgcacgcc aggccgcaag
     5281 cgaccgttgc cggcgcttca tggctcagga atttggcgaa gaaaaaattg tttcggtcta
     5341 cctggaaacg tttcagaacc tgctgcaacg aagactagat gcccgagtga ttccttcaag
     5401 tgagtcgccc catgcctaat acacctttta tcatcaccgt ggacaccgag ggcgacaatc
     5461 tctgggaacg tccacgcgag atcggtaccc gcaatgcgga attcctcccg cgcttccaaa
     5521 tgctctgcga gcgcttcggg ttcaagccgg tctacctgac caattatgag atggcaatgt
     5581 cggacgccta tgtggagttt gcgcgagacg tcttgtgccg ggatgcatgt gaaatcggca
     5641 tgcacctgca cgcatggaat tcgcctccgc tacaagcttt gaccgaggac gattttttcc
     5701 atcagccgta cctcattgaa tatccggata gcgtcatgag ggaaaaaatc cacgtcatga
     5761 cagcgttgct cgaagacaag ttcgaccgga aaatgattag ccatcgcgcc gggcggtggg
     5821 gtttcgatga acgttacgct gccatgctgt tgcaggaggg ttatcaggtg gactgctcgg
     5881 tgacgccagg tgtgaactgg ggtgcaaacc ggggtaaccc gaatggaaac ggaggctcca
     5941 actacgaagc gttccccgat catccttatt ttctcgaccc cgcaaacatt tccgcgcccg
     6001 ccccgtatgg tctgcttgaa gttcccatga ccattgtcct tagcagcctg taccgcaaag
     6061 catcctactt gtatcgcgtt cctttgctgc ggcgcgccgc aaacaggttg tccccgggcc
     6121 tgagctggtt gtgcccggtg caaccgtcgt tgggcgcgcc gcttgcccgc agcatggatg
     6181 tcatgttgca gatggcgcgg gcgacacgca atgaaggtgt tgcctgcatg gaattcatgc
     6241 tccactcatc tgaactcatg cctggcggaa gcccgaactt cgcaactccg tcagatatcg
     6301 atcaattata tgaccggctg gaaatgctgt tcgaagagct ttcgacctgg tgctatggcc
     6361 tgacactgca cgaattccgc gatctctgga tacagctgtg cggccacacc ggaatgcgcg
     6421 cgttcgcata ccagcagcct gatcagacag gtgccctgac cgccacggcg caattcaatg
     6481 cacgctcaat tccggtgggc aaacaggcca taccggaatg atccgtctcg agggccgtgc
     6541 tgcactatac ggtgacgtgt ggttcgaaga ggaactgcct gcggatgcgg gtgtggacat
     6601 cattcgctat cactgccgcc cgaagccggt agcgggcgcc cgatctttcg aatttttgtc
     6661 gatgaccaca aacctgctca tcgatccgga agcgatcggc gatgcattca gcaaggattg
     6721 ccgctacaaa ataagacggg ccgataccaa ggacggtttg gtcatggagt gcatcacgag
     6781 tccggagccc aggttggccg aattttgtgc gttctttgat gaattcgcca ggcagaaatc
     6841 gcatgatcct tccgaccgcc aatggttaca cgccgcctgc aaggccgggc aactggtgct
     6901 gacttcggca tcccgaagcg gcgaagtgct ggtctggcat gcttacctgc tttgtggcgc
     6961 ggcaacgtgg ctccaataca gcggttcctg tt
//
LOCUS       DATMZQ010000126         4867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3896577, whole genome shotgun sequence.
ACCESSION   DATMZQ010000126 DATMZQ010000000
VERSION     DATMZQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3896577"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VN496_03950"
     CDS             complement(<1..755)
                     /locus_tag="VN496_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF16130.1"
                     /translation="MSDLSKSSEDTPGARGWWSVIKKAANRLQDWFVIQTFATKLAVL
                     IIVVLIPATGIYSGMLLKRQALVAEQVRNMSVPGKAGEGIWTLNDKLPVVFGAGSMLS
                     FLEQNPIDRITVIYRPLTWSVSERIILIESQGKQYAYYPSDMESKIFADKAVSAPWGG
                     KLVFVPRAELTNSSRELLERFDQRFAGARPQSASDAWMAALSAALSGLLTIGLLTFLF
                     YQLKSQYKSLKFIEPGQVHGSIDDLVGMDDIKA"
     gene            complement(907..1689)
                     /locus_tag="VN496_03955"
     CDS             complement(907..1689)
                     /locus_tag="VN496_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HXF16131.1"
                     /translation="MWGMRISNFVFAVLAALPGLSAAADLTPDHVRKILLTPGHETGN
                     ALAGKDLGRLDLSRQDFTGANLVRTSFFASKLVQSKFSNARMARANLNGAWLMGTDFS
                     GADLSGASLMSVVILGGEVTVRPNFNHANLSGARVIAELDGADLSHANLSGAHLGVDI
                     KNQGMGQMRTNLIRANLEGAQLVGADLNRTLLRFANLKDVNLQGANLFRADLSGADLS
                     GADLTGANFEEANIDGTLFKGARGVDAALGLDRAEHRDRAKF"
     gene            complement(1722..3140)
                     /locus_tag="VN496_03960"
     CDS             complement(1722..3140)
                     /locus_tag="VN496_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXF16132.1"
                     /translation="MSSLTHRLLQWTETRIWRAPGASQSRAQAWLTHALRLTIVLARD
                     LALGQLTLRAMGLVYTTLLSIVPLLAISFSVLKAFGVSNQIRPVLLGFLQPLGDKGVE
                     ITMRITEFIDNINGGVLGSVGLALLLYTSVSLAQKIEEAFNFIWHVSRPRSIGERFSR
                     YVSALLVGPLLVFSALGLTAAAANLAVVREALRIEAIGWLASQATHLVPYALVIGAFA
                     FLYAFMPNTRVRSGPAIAGAFVGGVAWQSAGWAFAQFVVTSTKYSAIYSSFAILILFM
                     LWLYLSWLILLFGADVSFYVQHPEYVVKKSGEPRLSSRLRERLALVIMSLIARQHLEG
                     REAWSADALAHELHVPVRAVDGVLTTLQARGMLAATATEPCTWLPARALDVISTKEVI
                     DAVRAAGEEGSLSSDTLSAPATVDQLMQRYEQAAAIALRGISIRDLAASLPAASDGEH
                     AWTKRIADRRNAQREKGDRAAS"
     gene            complement(3175..3966)
                     /locus_tag="VN496_03965"
     CDS             complement(3175..3966)
                     /locus_tag="VN496_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXF16133.1"
                     /translation="MVWTIGYGGVALIVIVILTMTLRVLREYQRGVVFTLGRFTGVKG
                     PGLIVLIPGVQQMVRMDLRTVVLDVPSQDVISHDNVSVKVNAVVYFRVVNPEHAVIQV
                     ANFMEATSQLAQTTLRSVLGKHGLDEMLAERDKLNTDIQRALDAQTDAWGIKVSNVEI
                     KQVDLNESMIRAIARQAEAERERRAKVIHAEGELQASQKLLEAAQMLAQQPEAIQLRY
                     LQTLTQIAGDRSSTIVFPLPVDILTTLRHSLQRQPTGPSPEINRP"
     gene            complement(3971..>4867)
                     /locus_tag="VN496_03970"
     CDS             complement(3971..>4867)
                     /locus_tag="VN496_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXF16134.1"
                     /translation="SLRRKQINDSAAYIRGLAQSRGRNADWAERAVREAVSLSAREAL
                     ALKVVDVIAADLPQLLQRLNGRSVSAGGQKYVLTTLTAEVVQRDPDWRTQLLAVITNP
                     SIAYILLMGGLYGLLFEFMSPGFVLPGVVGGICLLLGLFALQLLPVNYAGLGLIALGV
                     ALFVTEFFTGASGVLGAGGLIALVAGSIMLIDTEAGAYAVPISLVAVIALLAAIGLFV
                     IARLAFASRRVPVVSGREQLLGSTGVVLTNDGEPYALIRGERWRVRSAAPLQVGDTVR
                     AVQLDGLVLTVEVLEAGPDRKE"
BASE COUNT         1000 a         1547 c         1414 g          906 t
ORIGIN      
        1 tcggccttga tgtcatccat gccgaccaga tcatcgatgg aaccgtgcac ttgccccggc
       61 tcgatgaatt tgagcgactt gtactggctc ttgagctggt agaacaggaa cgtgagcagc
      121 ccgatagtca gcaagccgga cagcgcggcg ctgagcgccg ccatccaggc gtcgctcgcg
      181 gactgcgggc gcgcgccggc aaagcgctgg tcgaagcgct cgagcagttc gcgactgctg
      241 ttggtcaatt ctgcgcgagg aacgaacacc agcttcccac cccagggcgc gctgacagct
      301 ttgtcggcga agatcttgct ctccatgtcg ctggggtaat acgcatactg cttgccttgc
      361 gattcgatca ggatgatgcg ctcggagacg ctccaggtca gcggccggta aatcaccgtg
      421 atgcgatcga tcggattttg ctccaggaaa ctcagcatcg aaccggcgcc gaacaccacc
      481 ggcagtttgt cgttcagcgt ccagatgccc tcacccgctt tacccggaac ggacatgttc
      541 ctgacctgtt cggcaacgag cgcctgcctc ttgagcagca tgcccgaata gatgccggta
      601 gccgggatca gcaccacgat aatcagcacc gccagtttcg tggcaaaagt ttggatgacg
      661 aaccagtcct gcaggcggtt ggcggccttc ttgatgacag accaccatcc ccgcgcgcct
      721 ggcgtgtctt cgctggattt cgataaatca gacatggcaa tccttacaaa aaatatggga
      781 ctgtcttatg ggcgcgcaaa gcaccgtgtt ccgcatcggg aacctgcgta cagtaacagt
      841 ccggccgttt acaggcaggc cttaccgata cgtctgcgct acgtcaggat gttacggttt
      901 atttttttag aacttcgccc ggtcgcggtg ctccgcgcgg tcaagcccta gcgctgcatc
      961 gacaccacgc gcacctttga acagcgtgcc atcgatattg gcttcttcga aattcgcacc
     1021 ggtcagatcc gcgccactca ggtcggcacc ggacaggtcg gcgcggaaca gattggcacc
     1081 ctgcaagttt acatccttca ggttcgcaaa gcgcagtaat gtgcggttga ggtccgcgcc
     1141 tacgagttgc gcgccctcca gattcgccct gatgagattg gtgcgcatct gtcccatgcc
     1201 ctgatttttg atatcaaccc cgagatgcgc acctgacaga ttggcgtggg ataagtccgc
     1261 tccgtcgagc tccgcgatca cgcgagcgcc ggaaagattg gcgtggttga aattcggccg
     1321 gaccgtcact tcaccgccaa ggatcacgac cgacatcagc gaggcgccgg agagatccgc
     1381 gccggaaaaa tcagtaccca tcagccaggc gccgttcaga ttggcgcgcg ccatccgggc
     1441 gttgctgaat ttgctctgca cgagtttgct ggcaaagaaa ctggtgcgga ccaggttcgc
     1501 tccagtgaaa tcctgccggc tcaaatccag acggcccagg tcttttccag cgagtgcgtt
     1561 accagtctca tgtcccggcg tcaacaggat tttccggacg tgatcgggcg tgagatcagc
     1621 agcagctgaa agtcccggca acgcggcaag aaccgcaaac acgaaattgg aaatgcgcat
     1681 gccccacatg atgcactctt tttatgatcg cggcgtacgt ctcatgaggc tgcgcggtcg
     1741 cccttttcgc gttgcgcatt ccgccggtcg gcgatgcgct tggtccacgc atgctcgccg
     1801 tccgacgctg caggcagcga agcggccaaa tccctgattg aaatgccgcg tagtgcgatt
     1861 gccgcggcct gttcgtaacg ctgcatcaac tggtcgactg tggctggagc tgacaaggtg
     1921 tcgctgctca atgagccttc ttcccccgcc gcacgcaccg catcgatgac ttcttttgta
     1981 ctaatgacgt cgagcgcgcg agccggcagc caagtgcacg gctccgttgc ggtagctgcc
     2041 agcataccgc gcgcctgcaa ggtggtcagc acaccgtcga ccgcgcgtac cggcacatgc
     2101 agctcatgcg ccagtgcatc tgcactccac gcttcgcggc cctccagatg ctggcgtgcg
     2161 atcaagctca tgatcaccag cgccagacgc tcgcgcaatc ggcttgacag gcgcggctcc
     2221 ccgcttttct tgacgacata ttcaggatgc tgcacgtaga agctcacgtc cgcgccaaac
     2281 aggaggatca gccagctgag gtatagccac agcatgaaca ggatcaggat tgcgaagctg
     2341 gagtagatcg cagaatactt cgtcgatgtc acaacaaatt gcgcaaaggc ccaccctgct
     2401 gactgccagg ccactccgcc gacgaaggcg ccggcaatgg ccggaccgga ccgcaccctg
     2461 gtgttgggca tgaaggcgta caagaaagcg aacgcgccaa tgaccagtgc atagggcacc
     2521 aggtgcgtcg cttgcgacgc aagccagccg atcgcctcga tgcgcaatgc ttcgcgcacc
     2581 accgcaagat tggccgcagc ggccgtaagg cccagtgccg aaaacaccag cagcgggccc
     2641 accaataatg ccgagacata ccggctgaag cgctcgccga tgctgcgcgg gcggctcaca
     2701 tgccagatga aattgaatgc ctcctctatt ttctgcgcca gcgatactga tgtgtagagc
     2761 agcagcgcca accctaccga gccgagaacg ccgccattga tgttgtcgat gaactccgtg
     2821 atgcgcatcg taatctcgac gcccttgtcg ccgagcggtt gcagaaaacc aagcagcacg
     2881 ggacgaatct ggttggaaac cccgaacgcc ttcagcaccg agaagctgat tgccagcagc
     2941 ggcacgatcg atagcaacgt ggtgtacacc aggcccatcg cgcgcaaggt cagctggcct
     3001 aacgcgagat cgcgtgccag cacaatcgtc agccgcagtg catgcgtgag ccaggcttgc
     3061 gcgcgcgact gagatgcgcc tggtgcgcgc cagatgcgcg tctccgtcca ctgcagcagc
     3121 cggtgcgtga gcgagctcat gcgctaaacg cttcagcgaa taacctgcct ggtttcatgg
     3181 ccgattgatc tcaggtgacg gtccggtggg ctgccgttgc aggctatggc gcagcgtcgt
     3241 gagaatgtcc acgggcaacg ggaacacaat ggtggacgag cgatcaccgg cgatctgcgt
     3301 cagggtctgc agatagcgca gctggatcgc ctcgggctgc tgtgccagca tctgcgccgc
     3361 ctccagcagc ttctgcgatg cctgcagctc gccttccgca tggatgactt tcgcccgccg
     3421 ctctcgctcg gcttcggcct ggcgtgcaat cgcgcgaatc atcgactcgt tgaggtctac
     3481 ttgtttgatc tctacgttcg agaccttgat gccccaggca tcggtttgcg catccagcgc
     3541 acgctggatg tcggtattga gcttgtcgcg ttccgccagc atttcatcga gtccgtgctt
     3601 gccgagcacc gagcgcagcg tggtctgggc aagctggctg gtcgcttcca tgaagttcgc
     3661 gacctggatg acagcgtgct cgggattgac cacccggaaa tacaccacgg cgttgacctt
     3721 taccgacacg ttatcgtgcg aaatgacatc ctggctcggc acatcgagca ccacggtgcg
     3781 cagatccatg cgcaccattt gctgcacgcc gggaatcaga acgatcaggc ctgggccttt
     3841 caccccggtg aaacgaccca gcgtgaacac caccccgcgc tggtactcgc gcagcacgcg
     3901 cagcgtcatt gtcaggatga caatgacaat cagcgccacg ccgccatatc cgatggtcca
     3961 aaccatgggc ttactccttt ctgtccggtc cagcttccag cacttcgacg gtcagcacca
     4021 ggccgtcgag ctggacggcg cgcactgtgt caccgacctg taatggggcc gccgagcgca
     4081 cccgccagcg ttccccacga atcagcgcgt agggctcgcc gtcattcgtc agtacgaccc
     4141 cggtagagcc gaggagttgc tcgcgcccgc ttactaccgg cacccggcgc gacgcgaacg
     4201 cgagtctggc aatcacgaac agcccgatgg ccgccagcag cgcaatcacc gctaccagcg
     4261 atattggcac ggcataggcg cccgcctcgg tatcgatcag catgatggac ccggcgacca
     4321 aagcgatcag cccgccggct cccaacaccc cggaagcgcc ggtaaagaat tccgtgacga
     4381 acagtgccac cccgagggca atcaatccca ggccagcgta gttgaccggt aatagttgca
     4441 aggcgaataa accaagcagc agacaaatcc cgcccaccac gccgggcagc acgaaacccg
     4501 ggctcatgaa ctcgaacagc agtccgtaaa ggccacccat cagcaggatg taggcaatgg
     4561 acggattggt gatgacagcc agcagctgcg tgcgccagtc cgggtctcgc tgcactactt
     4621 ctgcggtcag cgtggtcagg acgtacttct ggccgccagc ggagacggat cggccgttga
     4681 ggcgctgaag caattgcggc aaatccgccg cgattacatc gacgaccttc aacgcaagcg
     4741 cttcccgggc ggacaggctg accgcttcac gcaccgcgcg ttcggcccaa tccgcattgc
     4801 ggccacgcga ctgcgccagt ccgcgaatgt aagccgcgga atcgttgatc tgcttgcggc
     4861 gcaggct
//
LOCUS       DATMZQ010000127         5509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2515803, whole genome shotgun sequence.
ACCESSION   DATMZQ010000127 DATMZQ010000000
VERSION     DATMZQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2515803"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VN496_03975"
     CDS             <1..773
                     /locus_tag="VN496_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXF16135.1"
                     /translation="GTVHADRIVDQFTRQAVPFSTAKTIADRKALQLLIDVTGAAAED
                     TVLDVACGGGNVVCAFAARVRHATGIDMTPAMLQHAQATGKQQELTNISWDLGQATAL
                     PYADASFSIVVTRFSFHHFIEPLAVLKEMVRVCAPGGRVLVADMHTSTDPEQGAAFNR
                     MELLRDPSHVRALPIAELKALFPAAGLPDARITFYELRDELENLLARSFPNPGDADKI
                     RELFRMSAQDNRLGIPISIEGSRIHYGYPVAVLVATRR"
     gene            780..1379
                     /locus_tag="VN496_03980"
     CDS             780..1379
                     /locus_tag="VN496_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXF16136.1"
                     /translation="MAQDSSKTDFWETRYRDHITPWDAGKVPAALRDYVAKFEPGARV
                     LIPGCGFAHEAAFLADKGFDVLAIDFSPAAIATALKSLPESARFVQLADFFEFDFAKR
                     FDIIYERAFLCALPRTLWDRYAKRSAELLKPDGIIAGYFFFAESPKGPPFGTSRAELQ
                     ALLGQRFALVDDQPVTDSIDVFSGKERWMVWRKAEDRTP"
     gene            complement(1431..2216)
                     /locus_tag="VN496_03985"
     CDS             complement(1431..2216)
                     /locus_tag="VN496_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HXF16137.1"
                     /translation="MFGFRQWRRRRILRKSRIDDVTWRRVSSRFVFVARLHDDERARL
                     KELAILFLHDKQISAAGGLDLSPEMKLGIAVQACILVLELGIEHYDGWVEVIVYPAEF
                     VSRHEFRNPDGLVETDQTAYAGQAWLRGPVILSWASVAHAGDTDGMNVVIHEFAHKLD
                     MLNGTANGFPPLHAGMNRRLWSDVFNAAYQDLRRRVRAGESTEIDPYATESPGEFFAV
                     VSETFFEIPDIVKVVYPQVYEQLALFYRQDPASRQLPREWRLR"
     gene            complement(2237..3685)
                     /gene="rmuC"
                     /locus_tag="VN496_03990"
     CDS             complement(2237..3685)
                     /gene="rmuC"
                     /locus_tag="VN496_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXF16138.1"
                     /translation="MIEFSVGLGLSAATLFAVIVLIFMAASVVRRQTSHPDSLTRVLE
                     DKHMAMIKDLNAGLNGLGDRLNTAQLELFDRLRTTITQELTQTRSTVGALQVKQVEEL
                     SATRETTTLKLAQMTADLQVKHDQLRSEVLTRILQTLAEQNRAEQELIQTTMRNASAQ
                     LSASMESLSKTTDTRLEQISGKVTERLEEGFKKTNETFVSVMARLATIDEAQKKIDGL
                     TSNVVSLQELLGDKRSRGAFGEIQLEGLVRNILPAMAYEFQYTFSATGARADCVLKLP
                     PPTGMVAVDAKFPLENYRRMFEQGMPESERAAAQRQFRADVKKHVDDIAEKYIIAGET
                     SDGAVMFVPAEAVFAEIHANQSEVVDYAMQRRVWIVSPTTLMAVLNTARAVMKDVATR
                     EQVHIIKDELGKLGKDFQRFDARMKRLADHIRQAHQDAEEVRVSSDKITRRFVQIERV
                     ELDQPDQMRALELTMLDAPLEDEPPIGDNSGA"
     gene            complement(3884..>5509)
                     /gene="typA"
                     /locus_tag="VN496_03995"
     CDS             complement(3884..>5509)
                     /gene="typA"
                     /locus_tag="VN496_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXF16139.1"
                     /translation="HINIVDTPGHADFGGEVERVLSMVDSVLLLVDAVEGPMPQTRFV
                     TRKALALGLKPIVVVNKVDRPGARADWVVNQTFDLFDKLGATEAQLDFPVVYASALNG
                     YATMSLNEPSTDMRPLFDAILQYVPVRDADPDAPLQLQICSLDYSSFVGRIGIGRVAQ
                     GRIRSGQQVAVMHGPDSEPKIAKVNQVLVFKGLERTVVDEAEAGDIVLINGIEEVGIG
                     VTLTDPTNPKAFPLLKVDEPTLTMNFLVNTSPLAGRDGKYVTSRQIRERLERELQSNV
                     ALKVEFTADSDTFVVSGRGELHLTILLENMRREGYEMAVGRPRVVMRTVDGVKEEPYE
                     LLTVDVEDAHQGAVMEELGRRRGDLQDMQADGKGRTRLEYRIPARGLIGFQGEFLNIT
                     RGTGLASHIFDDYGPVKGDMEERRNGVLISQDDGEAVAYALWKLQDRGRMFVSPGDAL
                     YEGMVIGIHSRDNDLVVNPIKGKQLTNVRASGTDEAVRLVPPIELTLEKAVEFIADDE
                     LVEITPKSIRIRKRYLKEHERKRASRDTTTAAA"
BASE COUNT         1085 a         1697 c         1536 g         1191 t
ORIGIN      
        1 ctggtacggt acacgcggac aggatcgtcg atcaattcac ccgtcaagcc gtgccgtttt
       61 caacggcaaa aaccattgca gatcgcaaag cgctacagct gctgatcgat gtaaccggtg
      121 cggcggccga ggataccgta ctcgatgtcg cgtgcggcgg tggtaacgtg gtgtgcgcct
      181 tcgccgcaag ggtcaggcac gcaaccggta ttgatatgac gccggcaatg ctccagcacg
      241 cacaggcaac cggcaagcaa caggaattga ccaatatcag ctgggatctg ggccaggcta
      301 ctgcactgcc ctacgccgac gctagcttct ccatcgtggt cacgcgtttc agcttccacc
      361 atttcatcga acccctggcc gtgctgaaag aaatggtacg cgtgtgtgcg cccggcggac
      421 gcgtcctggt cgcggacatg cacacgtcaa ccgatcctga acaaggcgcg gcgttcaacc
      481 gcatggaact gctgcgcgat ccctcccatg ttcgggctct gccaattgcc gagctcaagg
      541 cactctttcc cgcggccggg ctgcccgatg cccgcatcac tttctacgag ttgcgcgacg
      601 aactggaaaa tttactggct cgttccttcc cgaatccggg cgacgccgac aaaatccgcg
      661 aactgttccg aatgtcggcg caggacaatc ggctcggcat tccgatcagc atcgaaggat
      721 cgcgcattca ctacgggtat ccggttgcgg tgctggtcgc tacaaggcgc tgaatgcgga
      781 tggcacagga tagttccaag acggatttct gggaaacgcg ctatcgcgac cacattacgc
      841 cttgggatgc cggcaaggta cccgcggcac tgcgcgatta cgttgcaaag tttgagcctg
      901 gagcgcgcgt gctgattccc ggctgtggat ttgcccatga agcggcgttc ctggccgaca
      961 aggggttcga cgtgctggcc atcgacttta gtcctgcggc cattgccacc gcactgaagt
     1021 cgttacccga atcggcgcgc ttcgtgcagc tcgccgattt ttttgaattc gatttcgcca
     1081 aacgcttcga tatcatttac gaacgcgcct tcctgtgcgc cctgccgcgc acgctgtggg
     1141 accgctacgc caaacgcagt gccgaactgc tgaaaccaga tggcatcatc gccggctact
     1201 tttttttcgc cgagtcgcca aaagggccgc cgtttggaac cagccgggcg gaactgcaag
     1261 cgttgctcgg gcaacggttt gccctggttg acgaccagcc ggtcaccgat tccattgacg
     1321 tcttctctgg caaagagcgc tggatggtct ggcgcaaggc tgaggaccga acgccctgag
     1381 ttttgtgcgc gcgccacagt ccggggcgat tatgctccac tacacggcct ttaccgtagc
     1441 ctccattcgc ggggcagttg ccggctcgcc gggtcctgtc gatagaaaag cgcgagctgt
     1501 tcgtaaactt gcggataaac gactttgacg atgtcgggga tctcaaaaaa agtttcgctg
     1561 accaccgcga aaaactcgcc aggcgattcg gtcgcatagg gatcgatctc ggtactctcc
     1621 cccgccctga cccgccggcg aaggtcctga taagcggcat tgaacacatc ggaccacaag
     1681 cgccggttca tgcccgcgtg caacggagga aagccgttag ccgtgccgtt cagcatgtcg
     1741 agcttgtgcg caaattcatg gatcacgaca ttcatgccgt ccgtgtcgcc cgcgtgcgcc
     1801 acgctcgccc acgagagaat gaccggaccg cgcagccacg cctgcccggc gtacgcagtt
     1861 tggtcggtct ccacgaggcc gtccggattg cggaattcat gccgcgaaac aaattccgcg
     1921 ggataaacga tgacctcgac ccagccatcg taatgctcga tgccaagttc gagcaccaga
     1981 atacaggcct gaaccgcgat gccgagtttc atttccggac tcaggtccaa cccgccagcg
     2041 gcagaaatct gcttgtcgtg caaaaacaga atcgccagtt ctttcaggcg agctcgctca
     2101 tcatcatgca ggcgcgccac aaaaacaaag cgcgatgaga cccggcgcca ggtaacgtca
     2161 tcgatgcggg atttgcggag gatgcggcgt ctgcgccact gcctgaaacc aaacatggga
     2221 acttgcgcgt tgccggtcag gcccccgagt tgtcacctat aggcggctcg tcttccagcg
     2281 gggcatcgag catcgtgagt tccagggcgc gcatctgatc gggttgatcg agctcgactc
     2341 gttctatctg gacaaaacgc ctggtgatct tgtcgctgga aacccgaact tcctccgcat
     2401 cctgatgcgc ctgccggatg tggtcggcaa gtctcttcat gcgcgcatcg aagcgctgaa
     2461 aatccttccc gagcttgccg agttcgtcct tgatgatgtg cacctgttca cgcgtagcca
     2521 catctttcat gaccgcccgc gcggtgttca ataccgccat caatgtcgtc ggtgagacaa
     2581 tccagaccct gcgctgcatt gcatagtcca cgacttccga ttggttagcg tggatttcgg
     2641 caaatacggc ttctgccggg acgaacatga ccgcaccgtc cgaagtttcg cccgcgataa
     2701 tatatttctc cgcgatgtcg tccacgtgtt ttttgacgtc cgcacgaaac tgccgctgcg
     2761 cggccgcgcg ttcggattcc ggcatgccct gctcaaacat gcgccggtag ttctccaggg
     2821 ggaatttggc atcgaccgcc accattccgg ttggtggcgg caatttcagc acgcaatccg
     2881 cgcgcgcgcc ggtcgctgaa aaagtgtatt ggaattcgta ggccatggcc ggaaggatat
     2941 tgcgtaccag gccttccagc tgtatttcac caaaggctcc gcgcgaacgc ttgtcgccga
     3001 gcagttcctg caggcttacc acattgctgg tgagtccatc tatttttttc tgcgcttcat
     3061 cgatggttgc caggcgtgcc atcacgctga cgaacgtctc gttggtcttc ttgaagcctt
     3121 cctccagccg ctcagtcact ttgccgctga tctgctcgag acgcgtgtcg gttgtcttcg
     3181 atagcgactc catggaagcc gacaactggg ccgatgcatt gcgcatggtc gtttggatta
     3241 gttcctgttc cgcgcgattc tgttccgcca gggtttgcag gatgcgggtc agcacttcgc
     3301 tacgcaactg gtcgtgcttg acttgcaagt cggcggtcat ctgcgccagt ttcagcgtcg
     3361 tcgtttcgcg cgtagcggaa agttcttcga cttgcttgac ctgaagcgcg ccgaccgtgc
     3421 tgcgcgtctg cgtaagttcc tgggtgatcg tggtgcgcag ccggtcgaac agttccagtt
     3481 gcgccgtgtt caaccggtcg ccgaggccgt tcagtcccgc gttcagatcc ttgatcatcg
     3541 ccatgtgctt atcctccagc acgcgcgtca gcgagtcggg gtggctcgtc tggcggcgca
     3601 cgacactcgc tgccatgaag atcaggacga tgacggcgaa tagcgtcgct gcagaaagtc
     3661 cgaggccaac cgagaattca atcatctcag gagtataact gaagcatcca ccgtctcgtc
     3721 ccggtccgcc caaccgcctc aaagaggccg gaacgaatca gaatgcagcc gcaaaaatgc
     3781 gattttttgc ggccacgctg cgatagagaa aggccaaaat aaagggcgac catacgcggt
     3841 cgccctggat tcatcgggcc agaatcagcc gtcaaattgt tcgttacgct gctgccgtgg
     3901 tcgtgtcgcg cgatgcgcgc ttgcgttcat gctccttgag gtaacgcttg cggatgcgaa
     3961 tcgatttcgg cgtgatttcc accaactcat cgtcggcaat gaattcaaca gccttttcca
     4021 gcgtcagttc gatcggcggt accagccgta ccgcctcatc cgtacctgaa gcccgcacgt
     4081 tggtgagctg cttgcctttg atcggattaa ccaccaagtc gttgtcgcgc gaatgaatgc
     4141 cgatcaccat gccctcgtag agcgcatcgc cgggcgacac gaacatacgg ccacgatcct
     4201 gcagtttcca caaggcgtag gcaaccgctt cgccgtcatc ctgtgagatc agcacgccat
     4261 tgcggcgttc ttccatatcg cccttgaccg gcccgtaatc gtcaaagatg tggctcgcca
     4321 ggccggtgcc acgcgtgata ttcaggaatt cgccctggaa cccgatcagc ccgcgcgcgg
     4381 gaatgcggta ttcaagccgt gtgcggcctt tgccgtcggc ctgcatatcc tgcaggtcgc
     4441 cgcgtcgtct gccgagttct tccattactg cgccctgatg agcgtcttcc acgtccacgg
     4501 tgagcagttc atacggttct tcctttacgc catcaacggt tctcatcacg acgcgtggac
     4561 gcccgaccgc catttcgtag ccttcgcgac gcatgttttc cagcaggata gtcagatgca
     4621 gttcgccgcg tcccgatacg acaaacgtgt ccgaatcggc ggtaaattcc actttcagtg
     4681 ccacattcga ttgcagttcg cgttccaggc gctcgcgaat ctggcggctg gtcacgtact
     4741 tgccgtcgcg accggccaaa ggtgaggtgt tgaccaggaa gttcatggtc agggtcggct
     4801 cgtctacctt cagcaatgga aatgcctttg gattggtcgg atcagtcagg gtaacgccga
     4861 tgccaacttc ttcgatgcca ttgatcagga cgatatcgcc cgcctcggct tcgtcgacca
     4921 ccgtgcgctc caggcctttg aataccagca cctgattgac cttggcgatt ttcggttcgc
     4981 tgtcgggacc gtgcatgact gcgacttgtt gccccgaacg aatccgcccc tgcgccaccc
     5041 ggccaatgcc gatacgacct acaaaacttg aatagtccag cgagcaaatc tgcaactgca
     5101 acggcgcgtc cggatccgca tcgcgtaccg gaacatattg cagaatcgcg tcaaatagcg
     5161 gccgcatgtc cgtactcggc tcattcaggc tcatcgtggc gtaaccgttc agcgcagacg
     5221 cgtatacgac cggaaaatca agttgcgctt cggttgcgcc aagcttgtcg aacaggtcaa
     5281 acgtctggtt cacaacccaa tcggcacgcg cgcccggacg atccactttg ttgaccacga
     5341 cgatcggctt cagcccgagc gcaagcgcct tacgtgtcac gaaccgggtt tgcggcatcg
     5401 ggccttccac tgcatcgacc agcaacaaca cgctgtccac cattgacaga acacgctcga
     5461 cttcgccgcc gaaatcggca tgtcccggcg tgtccacgat gttgatgtg
//
LOCUS       DATMZQ010000128         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1432164, whole genome shotgun sequence.
ACCESSION   DATMZQ010000128 DATMZQ010000000
VERSION     DATMZQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432164"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VN496_04000"
     CDS             complement(<1..762)
                     /locus_tag="VN496_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXF16140.1"
                     /translation="MKAAKRPLPEHTKEALHNGIPMFIQQLIDTLEMEQEPKAFVDRR
                     VSSTAVSSTAGVGRRKSSEMNKAAAIHGRELLDHGFTIDQVVHAYGDVCQSITDLAFE
                     LDEPFEIEEFRSLNRCLDEAIAGAVTAFSDERDNLNAEKQSKEVNIRLGALAHEMRNL
                     IQTATLSFAAIKAGTVGLAGSTGAVMDRTLVAMRDMVDGSVAEVRMDAGMPMHSQIFA
                     LADLILEVKISSTMEANIHKCVFSVSPVDPQLAVQA"
     gene            1164..1901
                     /locus_tag="VN496_04005"
     CDS             1164..1901
                     /locus_tag="VN496_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXF16141.1"
                     /translation="MSAPNPHPPTRNLLIGALPTATYERLLPHFDPVVLPLGWSVYES
                     GERMAHVYFPTEGIVSRLYVLQDGASAEMAITGNEGAVGISLFMGGESTISRAVVQSA
                     GYAYRLKAALVKSEFARGDEFQYLLLRFTQALITQMSQTAVCNRYHTIEQQLCRWLLL
                     SLDRLSTNQLVMTQELIANMLGVRRGGVTDAARHLQAAGLIEYARGKITVMDRPKLED
                     RACECYAVVKRESDRLLSHFSAVSSPR"
     gene            2095..>2502
                     /locus_tag="VN496_04010"
     CDS             2095..>2502
                     /locus_tag="VN496_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16142.1"
                     /translation="MDNHEVLTKQQPIIGAGALIAEQADLIGKVSDAESFLNTAKAML
                     DGAQPLQEVNPVPAIAVTFLCGFCAETALKAALAQTGTSTKVLKDIGHQLIQLWNEAV
                     SKGILDSVPPRWLEQLNSLHAGDYVLRYPMQLNG"
BASE COUNT          569 a          691 c          667 g          575 t
ORIGIN      
        1 ggcttggacg gccagttgcg ggtccaccgg ggagactgaa aacacgcact tatgtatatt
       61 cgcttccatg gtagacgaga ttttgacttc gaggatcaag tccgccagag caaaaatctg
      121 gctatgcatt ggcatgccgg cgtccatccg aacttcggca actgagccat caaccatgtc
      181 gcgcattgca accaatgtgc ggtccatcac tgcgcctgta gaacctgcca acccgaccgt
      241 acccgctttg attgctgcaa agctcagcgt ggcggtctgg atgagatttc gcatttcatg
      301 ggcaagggcg cccaatcgta tattcacttc cttgctttgc ttctccgcgt tgaggttatc
      361 gcgctcatcc gaaaacgccg tgacagcacc ggcgatggcc tcgtccaaac agcgattgag
      421 tgaacggaac tcttctattt caaaaggctc atctagctca aatgccagat cggttatgga
      481 ctggcagaca tccccgtagg cgtgcaccac ctggtcaatc gtaaagccat gatcgagtag
      541 ttcgcggccg tgaatcgcag ccgccttgtt catttcagaa gacttgcgcc gccccactcc
      601 agcagtactg gaaacagcag tactggaaac cctccgatcc acaaaagctt tcggctcctg
      661 ctccatttcg agcgtgtcga tgagctgctg aatgaacatc gggataccgt tatgcagggc
      721 ttctttggtg tgctcgggca agggccgttt tgcagctttc acccggcatc gctcgatcaa
      781 attggagtcg ttatctctca ggaattggta aagcatggta ccccctcgtg acctcgacgt
      841 tagtcggcgt gccgaacgga gtatgtgcgc taccggactt ataaaatctt ttttacaaac
      901 cattgctttt cgctaaagat ttcaacgcgc gatatgcggc ggtgtgccgt aaagcccgtg
      961 gtccgcagcg gttgctatgc ctatctcggc ccgactgccc cccagtttaa ttgcgagcgt
     1021 gctcggcctg atcagggaaa cgtgacccca gatcgccgac ttatctggaa gcagtatgcc
     1081 gatcctgaag cctaaagtgg aggtagcgaa cgccgacttt taaggtgtac gctaccgtac
     1141 atatactggt tgaacgagcc cgtatgtccg cacccaatcc gcaccctccc acaagaaacc
     1201 ttcttatcgg cgcgctccct acggcgacct acgagcgcct gctgccccac tttgatccgg
     1261 ttgtgcttcc gcttggctgg tctgtatatg aatccggaga acgcatggcg catgtgtatt
     1321 tccctaccga ggggattgtc tcccggctat acgttttgca ggatggtgcc tccgcagaaa
     1381 tggcgatcac cggaaatgaa ggcgcggtcg gcatttcctt gttcatggga ggagagagca
     1441 ccataagccg ggctgtagtg caaagcgcgg gatacgcata tcgcctgaaa gcggcgttgg
     1501 tgaaatcaga attcgcacgc ggcgacgaat ttcaatacct gctcttgcgc ttcacgcagg
     1561 cattgatcac ccaaatgtcg cagacagccg tgtgcaatcg atatcacacg atagagcaac
     1621 agctatgccg ttggttgctg ttgagcctgg accgactatc caccaatcaa ctggtcatga
     1681 cccaggagtt gatcgcgaat atgctgggtg tgcgccgcgg cggcgtcacg gacgctgcgc
     1741 gacaccttca ggctgctggc ttgattgagt acgcccgcgg caaaatcacc gtaatggacc
     1801 ggccaaagct ggaagaccga gcgtgcgaat gctatgcggt ggttaaacga gaatcggacc
     1861 gtttgctatc gcatttcagt gctgtatcaa gtcctcgcta agcgccccgc actagcttaa
     1921 gcgtagcagt tcatctggct gtccgaccgt tggcgtacat acggcaacca gaaacgggct
     1981 gtgaaaacag ccttttttat ttttcttctt gcgcgctgcc gcggcttggg aataatgcga
     2041 cgcatcaccg cgcggatgat ttgacggtgg cacatttccg accggagaaa aaaagtggat
     2101 aaccacgaag tcttgacgaa gcagcaaccc atcattggcg ctggagccct gattgccgaa
     2161 caagcagacc tcattggcaa agtaagtgac gccgaaagtt ttctgaacac ggccaaggcc
     2221 atgctcgacg gtgcgcagcc gctacaggaa gtcaatcccg ttccggctat tgcagtaacg
     2281 ttcctgtgtg gcttttgcgc tgaaaccgca ctgaaggccg cattggcaca gaccggcacg
     2341 tcaacgaagg ttctaaagga tatcgggcat caattgatcc aactctggaa cgaagccgta
     2401 tcgaaaggaa tccttgattc agtccctcct agatggcttg agcaattgaa ttcccttcat
     2461 gcaggggact atgtattgcg ctatcccatg cagttgaatg gc
//
LOCUS       DATMZQ010000129        15618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1827829, whole genome shotgun sequence.
ACCESSION   DATMZQ010000129 DATMZQ010000000
VERSION     DATMZQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15618
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827829"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VN496_04015"
     CDS             <1..478
                     /locus_tag="VN496_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF16143.1"
                     /translation="YLKVGLLSMIPWTASVISMIYWGAHSDRTKERRWHSASGFLVKM
                     VGLLLLALVPHWPIASMFALTLVTIGWGAYVVTFWSLPTAFLSGTAAAAGIALINSVG
                     NLGGYFGPDMIGRIRMATGGATEPAFYTLAGVALLGALIILFLPRTQLKAVPLKAA"
     gene            complement(539..1324)
                     /locus_tag="VN496_04020"
     CDS             complement(539..1324)
                     /locus_tag="VN496_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HXF16144.1"
                     /translation="MELGLTGQTAVVTGASKGIGLAIARGLAAEGCSLQIVARSAATI
                     ESAADSIRRQYPVQVKAHALDLSRSETVSALLVATGTPDILVNNAGAIPGGDLQAVDE
                     ARWRAAWDLKVFGYINLCRAYYAAMRSKRKGVIINVTGLAADRLDTGYIAGSTGNSAL
                     NAFTRTLGSHSIEDGIRVLAISPGAVETERLVTLMKTRAADKLGDPERWREFVKGLPL
                     GRPATVDEIANVVVFAASTRASYLSGTVITVDGGHNARSGAFT"
     gene            1614..1961
                     /locus_tag="VN496_04025"
     CDS             1614..1961
                     /locus_tag="VN496_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16145.1"
                     /translation="MKKFLVLYLAPASVIDDWKKTPPEKRKEPEEKMQREWKQWMSSH
                     AKMFTDPGAGAGKTKRVTAHGTTDARNDIMLYGIVEAESHADAAKAFEGHPHFTIPQA
                     SIEVMEINPLRGM"
     gene            2176..2697
                     /locus_tag="VN496_04030"
     CDS             2176..2697
                     /locus_tag="VN496_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXF16146.1"
                     /translation="MSALFRALVLAVVSAGTASAQTIGGAEETLKKKNILLPAPPSPV
                     ANYVPAVRVGNLLFLSGSGPDPSTPRGKVGKDLTLEQGYQAARSVGLNLLANAKANLG
                     SLDKVKRVVKVLGMVNSAPGFTDQPKVINGFSDLMVEVFGESIGKHARSAVGMAELPF
                     GIPVEIEMILEVE"
     gene            complement(3152..3574)
                     /locus_tag="VN496_04035"
     CDS             complement(3152..3574)
                     /locus_tag="VN496_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HXF16147.1"
                     /translation="MKRSIISLLVAGLFAGTATVVFAAGNTSDTEVKAAPKQAETSQS
                     RSADPNTKDAMNDTSKDAKQDKNTSDKSMQNQDKTAAAFNKADKDHDGTLDRKEAKKF
                     NKNAAKNFDAIDTDKDGTISMDEVNTYMAAHPGKKGNM"
     gene            4138..4596
                     /locus_tag="VN496_04040"
     CDS             4138..4596
                     /locus_tag="VN496_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXF16148.1"
                     /translation="MILLIALAAGFAGGYASRSFAHGAGNGMSEAMDPAQMDEHVQRM
                     VKHLAVEVDATPEQQEKLNVIAKNVAHDLAPLRGNMKRARQQALELLSADKIDHAAIE
                     NLRAAQLATMDSASRQLSQALADAADVLTVDQRKKLAERVQHFGHHWHHG"
     gene            4680..5384
                     /locus_tag="VN496_04045"
     CDS             4680..5384
                     /locus_tag="VN496_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF16149.1"
                     /translation="MLASAPSASRILLIEDDQRLAALVAEYLGEAGYRVSVTETGADG
                     LARALHEPFDAVVLDLMLPDMDGLDVCRRLRGDSTLPILMLTARGNAMDRVVGLELGA
                     DDYLPKPFEPRELLARLRAILRRGRSGASADVLRFGRLEIDRAARAVRVDGVIRELTS
                     YQFALLLALAQNAGRVLSRESLMEQLKGETLEAFDRSIDVHISRIRAVIEDDARKPRR
                     IVTVRGGGYVFAKQQD"
     gene            5387..6631
                     /locus_tag="VN496_04050"
     CDS             5387..6631
                     /locus_tag="VN496_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF16150.1"
                     /translation="MNRLYFRIYLAVLASLVVFALAAGLLWRTLGESGNMWQGGELVG
                     QFAQNILPPASTDRIGQQAALERIGAGLHADVSLFAPDRSLLASLGAPLSVPAENSEE
                     PWSGARIMHSPVWTIHLPDGRWLVVRRPMTVRHIPGLALALAIGLLLVVVAIAAHPVV
                     RRLTRRLERLQNGVETLGRGDLSARVKVEGHDEVARLAMSFNRAAARIEALLNAHKML
                     LANASHELRTPLTRIRMGVELLKADADPARRAALERDIAEIDNLIEGILLSSRLDALA
                     EPEVNEEVDLLALAAEECARYESCSVSGDSVILHGDPILLRRLLRNLVENGLRHGSPP
                     VNVAVAREAGHAALRVTDHGRGIIGSETEAVFEPFHRGRHTSAAGTGLGLSLVRKIAR
                     HHGGDARIEKNGPDGLTVLVTL"
     gene            6906..8624
                     /locus_tag="VN496_04055"
     CDS             6906..8624
                     /locus_tag="VN496_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXF16151.1"
                     /translation="MKFFTAHQRACSTSGFTGNTGQGAARSLRAATCALITTLASAGI
                     AVAADDNPANWPVYHGNSKSSRYSALDSINKSNVKKLKVAWIHQPGEITGGLQATPIV
                     IDGVLYYSSANNKVQALDAASGKELWRYQPKLDPIVNEIFFTASSRGVTVGHGKVYLA
                     TLDGRLIALDQKTGKEVWITQLVETRKKSGNNFTSPPTLAGDVLVIAPTSGDTAQRGK
                     IYAVDANTGQPKWEFDTIKDDPNSWPGNTGDTGGGGGWLPGTYDKETDTLYWGVGNPA
                     PDYFGDERKGDNLYTDSVIALNPRTGVLKWVRQELPHDVWDFDSTYETVLFERNGKKL
                     LFHPTKAGFVWVMERDTGKLHNVWPYSKTYNFVKTIDPKTGELIGRQDPQVGQEILIC
                     PWILGSRSWNHGAYSPKSGLWYNNVMEACNTVKTAKQDPSKLGPADLYYGVESVQMVP
                     PPNEPASGRFEARDPESGKVKWSVPYSVPGLGCVMATAGGLVFNADVFGTLNAYDADT
                     GNVLWNFNMGSGSRGGIVSYAVNGKQYIVVASGMYSHAVDFMPPVFPKIREANGGSAL
                     IAFTLE"
     gene            8643..9008
                     /locus_tag="VN496_04060"
     CDS             8643..9008
                     /locus_tag="VN496_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF16152.1"
                     /translation="MKSIASGFIGLAFAAVLLCVTHAKAMELAEADLANPDVIAAGKK
                     RFNRSCFYCHGFEGEGGKGASLQRRPDLTPEIIFNTISNGRVRGSSVMPPWKNTLSEE
                     EITQLVAYILSLRNIPEQK"
     gene            complement(9408..10625)
                     /locus_tag="VN496_04065"
     CDS             complement(9408..10625)
                     /locus_tag="VN496_04065"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXF16153.1"
                     /translation="MKKQMYEKMLPGARLWTGDMKIEADAIEQIRNVTQLPILAGKVA
                     VMADVHLGKGATVGTVIPTRAAIVPAAVGVDIGCGMLAVKTDLVAAHLPENLGRLRAQ
                     IERDVPVGFSFHKRPLAPEGSREALLLDRRMKDLEERYPKLRILDYMGRFDEPRMWRQ
                     LGSLGGGNHFVEICLDAEGAVWIMLHSGSRNVGNVIGQSAMRMAKQVAKRVDRSLPDR
                     DLAWLDEGTPEFDAYVEGLGWAQEYASLNRDLMLHLVHKALAKSVEHEVAFVGEVTNC
                     HHNYARIEEHFGENIWITRKGAVSARAGELGIIPGSMGAKSFIVRGKGEAESYCSCSH
                     GAGRRMSRTQARKSFTRQDLAIETAGVECRKDQGVIDEIPSAYKDIDAVIAAQTDLVE
                     VVATLKQILCVKG"
     gene            complement(11007..13469)
                     /locus_tag="VN496_04070"
     CDS             complement(11007..13469)
                     /locus_tag="VN496_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16154.1"
                     /translation="MSQIPRVAVVVVHGIADQRPGQTVREIVRLLCHGNEGPPRYLQG
                     ELHDVLVPVAKLEPGGSIASAGSAAQNPVSETKGADPARLRPGTPSGFYQVQQSTPAE
                     TPAAAKNPEDLGIALNDYLLGRLKLSEQEALYESTRVSLRRRIDSREVDVYEMYWADL
                     SRLGTGGLRVLSSLYQLFFHLNTLSADVIDQIALRNAGGAAWRMLQRLHAWLAWLMKA
                     PAALVQLCMLLMVLFGAMAFVPTEQQGQLLAALFGAAAIALAALASLCWLRETSQTRR
                     MAKLSLLLVSAVVSLAVAIAALRAGEWTDIIYFGACAITVALLGAYLIESYARVAHGV
                     RMLGNGLVVATVICLCVQAGNLLPSVSTQREWMHTAALNIAEGLLALVLLAWAVFVLI
                     QIVALLLGLWLGRASAKPVRASLHTARLTLVVSSALFALLSLVLWSVVSYVAGHALDD
                     LLYEPLIFGDGYRSAAIFLDERVHSLGGFFTPMVFAFTLLAAAGFLVLAPSLLEELFP
                     SVNVDAHGPRPGAEQLSERLGAWLGDGMRRLGAIFTALVPLGAIAGSVLYLAFIFRQF
                     AFSIGVADGIAQWLAGSLDRFQGETLVAAGKWLAGGALTIAALGSRFTQTFGRLRVVI
                     DAVLDIDNYFGDPPNRQPPRARIYSRYASLLAYLRECGYARFVIVSHSQGTVISADLL
                     RYLHVQGRLPEIVGATPISLVTVGSPLRDLYAERFPLLYGWMGLNQSGFASATPNASD
                     IGAVEWVNACRSGDYVGRFIWTPSGDVKRFRIATVDSSGKTDVRRAGDRTEFCLGAGG
                     HTHYFSDDAVALAVEIDRLIAS"
     gene            complement(13695..14759)
                     /locus_tag="VN496_04075"
     CDS             complement(13695..14759)
                     /locus_tag="VN496_04075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF16155.1"
                     /translation="MKKLRFLLIYASFALLSCGSASNAAAHDFNDSHFHLTNYVQEGP
                     SLPEFLRIMGERAGRVAVFGIPLQQKWDYFESGPRAPDYYLLTDAELYYYSFTDAIVA
                     DQYLHLSAKDRARFDPMITGFNPTDMYAVDHIRRVLLMYPGVFSGIGEFSVHKEFVSA
                     KVAGHTASLLNPALDRILTFAGETGLVVILHNDINTVRPAPGRPANFDDLKSVFRSHR
                     DASIIWAHTGLGRFVKPTSDHLNLLREMLSGEEYSHVNFDLSWDEVAKWVVADAATLD
                     AWVTLLQQYPDRFLFGSDMVAPKSQADYLKAYEAYQKLWERLDGQTARKVKAQNYERL
                     FDAARLKVRQWEDKQVKLKK"
     gene            14958..15524
                     /locus_tag="VN496_04080"
     CDS             14958..15524
                     /locus_tag="VN496_04080"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXF16156.1"
                     /translation="MTTATKEKAVNNGVNVEALFGAREALTKAPEAAKFKWRASCEWV
                     KGTQSRSTVEGFYGLGAEQKHKTKFSFHSDHPEVFASEDSGATPVEIVLASLAGCLTA
                     GIAAVAQARDIQLRSVTATLEAGMDIRGILGADPDVRNGFDGVKVTYKIDADASKDDI
                     KALVAQSQKRSAVYDILTNPTNVTVEVK"
BASE COUNT         3298 a         4628 c         4563 g         3129 t
ORIGIN      
        1 ttacctcaag gtcggattgc tcagcatgat cccctggacc gcaagtgtca tctcgatgat
       61 ctactggggc gcgcactcgg accgcacgaa agaacgccgc tggcactccg cctcgggttt
      121 cctggtcaag atggtcggcc tgttgctgct tgcgctggtg ccgcactggc cgattgcatc
      181 gatgttcgcg ctgacgctcg tgaccatcgg atggggcgct tacgtcgtga cgttctggtc
      241 ccttccgacc gcgtttctga gcggtaccgc agcagctgcc ggcatcgcgc tgatcaactc
      301 ggtaggaaat ctcggcggct atttcggccc cgatatgatc ggtcgcattc ggatggcgac
      361 tggtggcgcg acggagccgg cgttttatac cctggcggga gtggcgctgc ttggcgccct
      421 gatcatcctg ttcttgccca ggacccagct caaagccgtg ccgctaaagg cagcgtgagt
      481 tattggtgaa tgaaaatcgg gcgctcggcg cccgattttt atgtgcttcg acgcgctatc
      541 aggtaaacgc cccgctccgc gcgttgtgcc cgccatccac tgtgatcacc gttccactca
      601 gatacgacgc tcgcgtcgaa gccgcgaaca ccacgacgtt cgcgatttca tctacggtcg
      661 caggccggcc aagcggtaat ccctttacga attcgcgcca gcgctccgga tcgccgagct
      721 tgtctgcggc acgcgttttc atgagcgtaa caaggcgctc cgtctcgaca gcaccagggc
      781 ttatcgccag tacgcgaatg ccatcctcga tactgtgact tccgagcgtg cgcgtgaacg
      841 cgttgagagc ggaattgcct gtcgacccgg cgatgtaacc ggtgtcgagc cgatcggcag
      901 caaggccggt cacgttgatg atcacgcctt tgcgcttgct gcgcatcgcg gcatagtaag
      961 cgcggcacaa gttgatgtag ccgaacactt tcaggtccca ggcagcgcgc cagcgcgcct
     1021 catccactgc ctgcaggtca ccgcccggta tggcgccggc gttattgacg aggatgtccg
     1081 gtgtgccagt ggcaacgagc aaggcgctga cggtttcgct gcgcgacaga tcgagcgcgt
     1141 gcgcctttac ttgaactgga tactgcctgc gaatgctgtc cgcggcgctt tcgatggttg
     1201 cggcgctgcg cgcaacaatt tgcaatgagc atccttccgc cgctaatcca cgtgcgatcg
     1261 cgaggccgat tcccttggaa gcaccggtaa cgacggcggt ttgtcctgtc aggcccagct
     1321 ccacgcgctc cccctctcgt ttgaatgctc ttgtgcgacg attgggaaat atagcatccg
     1381 ccgtcgaggg actccgtctc ttccattccg atagggcgac gccatcgcca atacttgcgc
     1441 cttggttacc tcattgaaat aacatttgcc gtttctctag cccggggatt gcgtgccgcc
     1501 cacccgcctt tcggtaaaat gtcgcaccag cacgaccatt gtgaagtcgt gactgtccaa
     1561 accaggcaag atctgtcgaa cggtctggat gcgataatct tgaaggagtc cctatgaaga
     1621 aattcctcgt tttatatctg gcacctgcct cagtgatcga cgactggaag aagaccccgc
     1681 ccgagaaaag aaaagaaccg gaagaaaaaa tgcagcgcga gtggaagcag tggatgagca
     1741 gccacgcaaa gatgtttacc gaccccggag ccggcgccgg gaagactaag cgcgtgactg
     1801 cccacggcac cacagacgcc aggaacgaca tcatgctgta cggcatcgtg gaagcggagt
     1861 cgcatgcaga tgccgcaaag gcgttcgaag gccaccctca tttcacgatt ccgcaggcat
     1921 ccatagaagt catggagatc aatccgctgc gcgggatgtg atgagggcga cgtgccccct
     1981 gcggggattc cgcatgggat accgtgcttt cgcacataac cggtctaaaa gaggtgggta
     2041 ccggtcttcg accataacga tcgtaaatgg taagggcgta aggccgtgcc ccctacgggg
     2101 ataccggtct tcgaccctaa tatcgtgaat acgtgaatgt cgtgaagtaa ccgaattttg
     2161 agaaggagaa aaatgatgag cgcactgttc agagcactgg tcctcgccgt agtctcggct
     2221 ggcacggctt cggcgcaaac gatcggcggc gccgaagaaa ccctgaagaa gaaaaacatc
     2281 ctgctgcctg cgccgccgtc gccagttgcg aactacgtac cggccgtccg cgtcggtaat
     2341 ctgcttttcc tgtcgggttc cggacccgat ccctctacgc cccggggcaa ggtcggcaag
     2401 gatcttacgc tcgagcaagg ttatcaggcc gcgcgttcgg tagggttaaa tttactcgcc
     2461 aatgcaaaag ccaatttggg aagtctcgac aaggtaaagc gcgtcgtgaa agttctcggt
     2521 atggtgaact cggcgccagg atttactgac cagccgaagg tcatcaacgg tttttccgac
     2581 ctcatggtgg aagtgtttgg tgaatccatc ggcaagcatg cccgctcagc ggtcggcatg
     2641 gccgagctgc ccttcggcat cccggtcgag atcgaaatga ttctcgaggt cgagtagcag
     2701 tgagctacgt caagaattaa aaaaatagcg caccacgaat accctggcgt ttagcccgga
     2761 gttatgggcg gaatacccta gcgaagctcg gagtaccctt ttagcccggt acccactgga
     2821 cccttttagg cacaaatacc ctcgcgctag cgtggagtag cccgttagac acgaagaaag
     2881 gcaagtgaga tatggtcggt ctaatgtggt tggccactga tgcaggcgcc agagagggaa
     2941 cgacaacgta ccgttagatc acaaataccc cttcagtccc ggttttcttc gtgtccctta
     3001 gcgtctttgt aagtcgcact gcgtttagca tcccagtgtc tcgggtttct tcgtgacctt
     3061 cgtgtctttg tggtggccac ttctttagtt cgtaagaaaa aaaacgggca cctgccggtg
     3121 cccgtcttta gttagtgcca gtgccctgca attacatgtt gccttttttg ccgggatggg
     3181 ctgccatgta cgtgttgacc tcgtccatgg atatcgtgcc atctttatcc gtgtcgatcg
     3241 cgtcgaaatt cttcgcagcg tttttgttga atttcttcgc ttccttgcgg tccagcgtgc
     3301 catcgtggtc cttgtcggcc ttgttgaacg cggccgccgt cttgtcttgg ttctgcatgc
     3361 ttttgtcgct ggtgttcttg tcctgcttgg catccttgct cgtatcgttc atcgcatctt
     3421 tggtattcgg atccgccgag cgcgactgcg acgtttcggc ttgcttgggt gcagccttga
     3481 cctcggtgtc tgaagtgttg ccagcggcga agacgacggt tgcagtgccg gcaaacaatc
     3541 ctgctaccaa cagcgaaata atggaacgtt tcatactcat gctcctttct ttgatgggaa
     3601 aaacgcggtc atgtttggtg gccgcgggcc gcattgacgc ggttgttcgg ctacctgtat
     3661 gcgaccaccg ggatcgtcaa aagttacggg acatcgcacg cggcaccccg gtaagacgta
     3721 ggccgagtag cctaatggac gtaggacgct cgcctacaac aagtaagcta ttgcacacag
     3781 aggtatgaaa ggatgttcag ataacgcaag accacgcgtc gccacgcaag gcgatgtggc
     3841 ttggacggca cggtgcgctc gaccgcatgg gtgcctggct cgatcactga atttttgcag
     3901 cgatcgagaa accgccaaac cagtgcgtac caaagcgtat tcgcgtcgcc gctttacaaa
     3961 tctttacctt ggcgagaaca ggcgttaacg ctgggccgtg aggatggcga catcgggcaa
     4021 tggtgctcga cccttcaata cggagttaac atggaaaccc tggaaacgtc cccgcaatcc
     4081 tcccattcac gttgcagcca ctccggacat cggggcgcgc gcttcggcag aggccttttg
     4141 attctgttga tcgcactcgc cgccggcttc gccggcggtt acgccagcag atcgtttgca
     4201 cacggagccg gcaacgggat gtcggaagcg atggaccctg cgcaaatgga tgagcatgta
     4261 cagcgcatgg tgaagcacct tgcagtggaa gtggatgcca cgccggaaca gcaggaaaaa
     4321 ctgaatgtga ttgcgaaaaa cgtggctcac gacctggcac cgctgcgcgg caatatgaag
     4381 cgagcgcgcc agcaagcgct ggaattgctg agcgccgata aaattgacca tgccgctatc
     4441 gagaatcttc gcgccgcaca gcttgccaca atggattccg cgtcccggca gctgagccag
     4501 gcgctggcgg acgcggcgga tgtattgaca gtcgaccaac gcaagaagtt ggccgagcgc
     4561 gtgcagcact tcggccatca ttggcaccac ggctaacgta acgttgcatc ggccaacgca
     4621 cgggcgcgca cttgttggct tcgacaaggc gcgtcggtgc gggtaaattc ccgtccaccg
     4681 tgctcgcctc cgctccatct gccagtcgca tcctgctgat cgaggatgac cagcgtcttg
     4741 ccgccctcgt agctgaatac cttggcgagg caggttatcg cgtcagtgtc acggagacag
     4801 gcgcagatgg acttgcccgc gccctgcacg agcccttcga tgcagttgta ctcgatctga
     4861 tgctgccgga catggatggc ctggacgtgt gtcgcagact gcggggcgat tcaacgttgc
     4921 cgattctcat gttgaccgcg cgcggaaacg cgatggatcg cgtcgttgga ctggagctgg
     4981 gcgccgatga ttatctgccg aaacctttcg aaccgcggga actgctggcg cgcttgcgtg
     5041 cgatcctgcg gcgcggccgc tcgggtgcct ccgccgacgt gctgcgtttc ggccgactgg
     5101 agatcgaccg tgctgctcgt gcggtgcggg ttgatggcgt gatacgtgaa ctgaccagct
     5161 atcagttcgc gttgctgctg gcattggcgc aaaacgctgg ccgcgtgctg tctcgagagt
     5221 cactgatgga gcaactcaag ggcgaaacgc tggaagcttt cgaccgctcc atcgatgtgc
     5281 atatctctcg cattcgcgcc gtcatcgaag acgatgcgag gaagccgcgt cgcatcgtca
     5341 cggtccgcgg cgggggctac gtgttcgcaa agcagcagga ttagccatga accgcctgta
     5401 cttccggatt tatctggcag tactcgcaag cctggtcgtc tttgcgctgg cggcgggact
     5461 gttgtggcga acactcggcg aatcggggaa catgtggcaa ggcggtgaac tggtgggaca
     5521 gtttgcgcaa aacatcctgc cgccagcctc gaccgatcgg attgggcagc aggccgcgct
     5581 ggaaagaatc ggcgccggac tgcatgcgga tgtttcgtta ttcgcgccgg atagaagcct
     5641 gcttgcgtcg ctgggtgcgc cgctttctgt gcccgctgaa aacagcgaag aaccctggtc
     5701 tggcgcacgc ataatgcact cgccagtctg gacgattcat ctcccggacg ggcgctggct
     5761 ggtcgtgcgc cggccaatga cggttcgcca cattccgggg ctcgcactcg cactagccat
     5821 cgggttgctg ctggtggtag tggcgatagc ggctcatcca gtggtacgcc gcctgacccg
     5881 ccggctggaa cgattgcaaa acggcgtgga aacactgggg cgcggtgatc tttcggcgcg
     5941 cgtgaaagtc gaggggcacg atgaagtcgc gcgactggcg atgagcttca accgtgccgc
     6001 cgcgcgcatc gaagcgttgc tgaatgcgca caagatgctc ctggccaatg catcgcacga
     6061 gctgcgcacg ccgctcacgc gcattcgcat gggcgtggag ctgctgaagg cagatgccga
     6121 ccccgcgcgc cgcgccgcgc ttgagcgaga catcgcagag atagacaacc tcatcgaagg
     6181 tatcctgctg agcagccggc tcgatgcgct tgcagagccc gaagtcaatg aagaagtaga
     6241 tttgctcgcg ctggctgccg aagaatgcgc gcgctacgag agctgcagcg tgagcggcga
     6301 ctcggtaata ttgcatggcg atcccatctt gctacggcgg ctgttacgca atctggtgga
     6361 gaatgggttg cgtcacggca gtccgccagt caacgtagcg gtcgctcggg aagccgggca
     6421 cgcagcgctg cgcgtaactg accacggccg cggaatcatc ggctctgaga ctgaggccgt
     6481 gttcgaaccc ttccatcgcg ggcgccatac cagcgccgcg ggcacgggcc tgggattgtc
     6541 gcttgtgcgc aagattgccc ggcaccatgg gggcgatgcg cgcatcgaaa agaatggacc
     6601 cgatgggctg acggttctcg tgacgctgta gcaactggcg attagcaaat tgcagggtca
     6661 cctggtttgc tgcgtcgcaa cgggttatgc gctgaatgat cagtctgata tgtcttgcgc
     6721 ggagctactc atcctttttt tgttccgcta gcatggtccg cgaacatgag gtcggaaagc
     6781 gggtaatcca aaacaaaatt cgactgcatt caggcaggag cttgtaaacg tagaacggcc
     6841 cttaccgatg cgccgcacgg tgagtcaaaa aaacatgtct tgcgtgtttc gaggaggagg
     6901 attccatgaa attctttact gcgcaccagc gcgcgtgctc cacgtctggt ttcaccggca
     6961 acaccggaca aggggccgct agatcgcttc gagccgccac ttgcgcgctc atcacaacgt
     7021 tggcctcggc cggtatcgcc gtggcagcag acgataatcc ggcgaactgg ccggtctatc
     7081 acggcaattc caaatcgagc cggtacagcg cgctcgacag catcaacaaa tccaatgtca
     7141 agaagctcaa ggtcgcatgg atccaccagc ccggcgaaat caccggcggc ctgcaggcca
     7201 cgccgatcgt catcgatggt gtgctctatt actcctccgc caataacaag gtgcaggcgc
     7261 tcgatgccgc aagcggcaag gaactgtggc gctaccagcc caagctcgat ccgatcgtga
     7321 acgaaatttt cttcaccgca tcgagccgcg gcgtcacggt tggtcacggc aaggtctatc
     7381 tggcgacact cgatggccgc ttaatcgcgc tcgatcagaa gactggcaag gaagtctgga
     7441 ttacgcagct cgtggaaaca cgcaagaagt caggcaacaa tttcacctcg ccacccacgt
     7501 tagctggtga cgtgctcgtc atcgcgccga cgtcgggcga taccgcgcaa cgcggcaaga
     7561 tctatgcggt cgatgccaac accggccagc cgaagtggga attcgacacc atcaaggatg
     7621 accccaacag ctggcccggc aacaccggcg ataccggcgg aggtggcggc tggctgcccg
     7681 gcacttatga caaggagaca gacaccttgt actggggtgt aggcaacccc gcgccggatt
     7741 acttcgggga cgaacgcaag ggcgacaatc tgtacaccga ctcggtaatc gcgctgaacc
     7801 cgcgaacggg cgtgctgaaa tgggttaggc aagagctgcc gcacgacgtc tgggacttcg
     7861 attcgacata cgagaccgtg ctgttcgagc gcaatggcaa gaagctgctg ttccatccga
     7921 ccaaggccgg atttgtctgg gtcatggaac gcgacaccgg caaattgcac aacgtctggc
     7981 cgtacagcaa gacttacaac ttcgtgaaga ccatcgaccc gaagaccggc gagttgattg
     8041 gccggcagga tccgcaagtc gggcaggaga tcctgatctg tccgtggatc ctgggttcac
     8101 gcagctggaa ccatggcgct tatagtccca agagcggcct ctggtacaac aacgtgatgg
     8161 aagcctgcaa caccgtgaag acggcgaagc aggatccaag caaactcggc ccggcggatc
     8221 tgtattacgg ggtcgaaagc gtgcagatgg tgccgccgcc gaacgagcct gcgtccggac
     8281 gtttcgaagc gcgcgatccg gaatccggca aagtcaaatg gagcgtgccc tattccgtgc
     8341 cgggcctcgg ttgcgtcatg gctaccgccg gcggcctcgt gttcaacgca gatgtgttcg
     8401 gcaccttgaa cgcctacgac gcggacaccg ggaacgtgct gtggaatttc aacatggggt
     8461 ctggctcgcg cggcggaatc gtcagctacg cggtcaatgg caagcagtac atcgtcgtgg
     8521 cgagcggcat gtattcccac gcggtggatt tcatgccgcc cgtgttcccg aaaatcaggg
     8581 aagccaacgg cggttccgcg ctgattgcgt tcacgctgga gtaatgccaa aaggagcgtg
     8641 gattgaaaag cattgcatcc gggtttattg gcctggcttt cgccgccgtt ttgctctgtg
     8701 tcacgcacgc aaaggcgatg gagttagcgg aggcagacct tgcgaatccg gatgtgatcg
     8761 ctgcaggcaa gaagcgtttc aaccgctcct gtttctactg tcacggcttc gaaggcgaag
     8821 gcggcaaggg ggcaagcctg cagcgccgcc ccgatctcac gcccgaaatc atcttcaata
     8881 ccatttcgaa tggcagggtt cgcggttcct ctgtcatgcc accgtggaaa aacacattaa
     8941 gcgaggagga gattacccag ctcgttgcgt atatcctgtc actgcgcaat attcctgaac
     9001 aaaagtaagg aagagctcgc attcggccaa ctttgcgaaa aaaaagacgg gcgcgattgc
     9061 gtccgtcttt ttgccgtacc ccgctcaggt tctgaaagaa gtaatcccgg tccgatccgt
     9121 cgaattcacc gctcagcatg caacacttac tgaaaccggc ggccagatgc gcacgggcac
     9181 ggatcgttac ggcccagttt ctcactgagt tccttgttgc cgttgacgat ccggtttccg
     9241 cgtttcacgc gggcctctga cgggaaacct ttgcggcgct tactcttggt ttcgaaacca
     9301 gaatacgccg gcattcctga gttcgtactt catgacacac ctcccatcgc tctaaaacaa
     9361 attgaacggg tgcatatctt cagcgccctt cctgaggcaa ggttgtccta tcctttcaca
     9421 caaagaatct gcttcagtgt cgcaactact tctacaaggt cagtctgcgc cgcaatgact
     9481 gcatcgatgt ctttgtacgc cgacggaatc tcgtcgatca cgccttggtc cttgcggcat
     9541 tcaacgcccg cggtctcgat agcaaggtcc tggcgcgtga atgacttgcg cgcctgcgtg
     9601 cggctcatgc gccttcccgc gccgtgcgag caagaacaat acgactctgc ctcgcccttg
     9661 ccgcgcacga tgaagctctt cgcgcccatc gatcccggga tgatgcccag ttcgccagcg
     9721 cgcgcgctca ccgcaccttt gcgcgtgatc cagatgttct cgccgaagtg ctcctcgatg
     9781 cgcgcatagt tgtggtggca gttcgtcact tcgccgacga aggcgacctc atgctccaca
     9841 cttttggcaa gcgccttgtg cacgaggtgc aacatcaggt cgcggttgag cgacgcatat
     9901 tcctgcgccc agcccaatcc ctcgacgtag gcgtcgaact ccggcgtacc ctcatcgagc
     9961 cacgcgagat cccggtcggg caaactgcga tcgacccgtt tggcgacttg cttcgccata
    10021 cgcattgcgc tctggccaat gacattgccg acattgcgcg agcccgaatg cagcatgatc
    10081 cataccgcgc cttctgcatc gaggcaaatc tccacgaaat ggttaccgcc gccaagcgag
    10141 ccgagttgcc gccacatgcg cggctcatca aagcggccca tatagtccag gatgcgcagc
    10201 ttcggataac gctcttcgag gtccttcatg cgcctatcga gcaggagcgc ttcgcgggag
    10261 ccttcgggcg ccagcggccg cttgtggaag ctgaatccca ccggcacgtc gcgctcgatt
    10321 tgcgcacgca agcgacccag attttccggc agatgagcgg caaccaaatc cgttttgacg
    10381 gcgagcattc cgcagccgat gtcgacgccc accgcagccg gcacgatggc tgcgcgggtc
    10441 ggaatgacgg tccctacagt tgcacctttg ccaagatgca cgtccgccat caccgcgact
    10501 tttcccgcca gaatcggaag ctgggtgacg ttacggatct gctcgatcgc atccgcttcg
    10561 attttcatgt cgcccgtcca caagcgggcg cccggaagca ttttttcgta catctgtttc
    10621 ttcatgatct attcccttac tgctctgatg atagtttgca ccccagcccg ccgccaatat
    10681 caaatccggc gccaaaaaca aaagcccgga gctctttcga gtctccgggc ttcgtggtgt
    10741 gcccacgctg acggatggcc ttatgccatc gccagccctc cggagacaaa gtcgatcccg
    10801 gatgccgagg atgtgcgcga cgcgatgacg cgcacgagct ctggcgcccg tggtttattc
    10861 atgcgttggt ctgaatatcg atccatctcc ggtcccaatg tgttgctgca ttttcgagga
    10921 cgcgcatcct gcgccagaga aaccagcatg tcaacgtatt tctgccgcaa cacgaaaata
    10981 ttgcgccacc gcaggattca gctcgatcaa ctggcaatga gccgatcgat ttcgactgcc
    11041 agcgccaccg cgtcatcgct gaagtaatgg gtatgaccgc ccgcgcccag acaaaattcc
    11101 gtgcgatcgc cggcacgcct gacgtcggtc ttgccactgg agtcaacggt ggcgatgcgg
    11161 aagcgtttta cgtcgccgga cggggtccag ataaaacgtc cgacatagtc gcccgatcga
    11221 caggcattaa cccattccac tgcgccgatg tccgaggcgt tcggcgtggc agacgcgaag
    11281 ccgctctggt tcaatcccat ccagccgtac agcagcggaa agcgttccgc gtacaggtcg
    11341 cgcaacggtg atccgacagt gaccagcgaa atcggagtcg cgccgactat ttccggcaag
    11401 cgcccctgca catgcaggta acgcagcagg tccgcgctga ttacggtgcc ctgactgtgc
    11461 gaaacgataa cgaagcgcgc atagccgcac tcgcgcaggt aagcaagcag cgacgcgtag
    11521 cgtgaataga tgcgcgctcg cggcggctgc cggttgggcg ggtcgccgaa atagttgtcg
    11581 atgtccagga ccgcatcgat aacaacccgc agccggccga aggtctgcgt aaagcgcgaa
    11641 cccagtgcag caatcgttaa ggctcctccg gcaagccatt tgccagcggc caccagtgtt
    11701 tcaccctgaa agcgatccag gctaccagcc agccattgcg caatgccgtc cgccacacca
    11761 atggaaaagg caaactgcct gaagatgaat gcgagataga gcacgctgcc cgcaattgca
    11821 ccaagcggca ccaacgccgt gaagatcgcg cccagccgcc gcatgccatc gccaagccag
    11881 gcgccgaggc gctccgacag ctgttcagcc ccgggacggg gaccgtgggc gtcgacattg
    11941 acgctgggaa aaagctcttc gagcagtgaa ggcgccaata ccagaaaccc ggcagcagcc
    12001 agcagcgtga aagcaaacac catcggcgtg aagaagccgc caaggctgtg cacgcgctca
    12061 tcgagaaaga tggcggccga gcggtatccg tcgccgaaga ttaacggttc ataaagcaga
    12121 tcatcgagcg cgtggcccgc aacgtagctg acaactgacc acaacacgag gctcagcagc
    12181 gcaaaaagtg cgctggaaac caccagcgtc aggcgtgccg tatggagtga tgctctgaca
    12241 ggcttggcgc ttgcgcgacc gagccataat ccgagcagca gcgccacgat ttgaatcagc
    12301 acaaatacgg cccacgccag taatacgagt gccagcaatc cctctgcaat attcaatgcg
    12361 gctgtatgca tccattcgcg ttgtgtgctg accgaaggca acaggttccc tgcctgtacg
    12421 cacaggcaga tgaccgtcgc gacgaccagt ccatttccga gcatgcgtac gccgtgcgcg
    12481 acacgcgcgt aactctcgat aaggtaagcg cccagcaggg cgaccgttat cgcacaggca
    12541 ccgaaataaa taatgtcagt ccattcgcct gcccgcaaag cagcgatggc aaccgccaga
    12601 ctgacgacgg cgctgaccag cagcaacgac agcttcgcca ttcgccgtgt ctgggatgtt
    12661 tcgcgcaacc agcaaagtga ggcaagcgcc gccagggcga tggccgcggc gccaaacagc
    12721 gccgccagga gctgcccttg ttgctccgtt ggcacgaacg ccattgcccc gaacaggacc
    12781 atcaacagca tgcaaagctg aacgagcgca gccggtgcct tcatcaacca cgccagccac
    12841 gcatgcagtc gctgcagcat ccgccatgcc gcgccgcccg cgttgcggag cgcaatctga
    12901 tcgatgacgt cggctgagag cgtattgaga tgaaagaaca gttggtaaag cgatgacaga
    12961 acacgcaggc cgcccgtgcc gagacgtgac agatcggccc agtacatctc atagacatcc
    13021 acttcacgac tgtctatccg gcggcgcagt gacacgcggg tagattcgta gagcgcttcc
    13081 tgttcggaca actttagccg acccaacagg taatcattta gcgcgatccc gaggtcctct
    13141 ggatttttcg cagcggccgg cgtctcggcg ggggttgact gctggacctg ataaaacccg
    13201 cttggcgtgc caggccgcaa tctggcggga tcggcgccct tggtttcgga aacgggattt
    13261 tgcgcagctg agccagcaga tgcaattgag ccgcccggtt cgagttttgc caccggaacc
    13321 agcacatcgt gcaactcgcc ctgcaagtaa cgtggcggac cttcattacc gtggcacagc
    13381 agccgcacga tctcgcgcac cgtctggccg gggcgctgat cggcaatgcc atgcactacg
    13441 actactgcga cgcgcggaat ttggctcact gcgtagtgcg aaccgatgcg gcgcaatggt
    13501 tgccatcgtt gcgccagcct tgggacggcg tcaattgcat ggacgtggta tcgcaatagt
    13561 actttgtcat catcggtccg cggaactcta acgctgccac gccggagggt caatgcacat
    13621 ggagcgggcg ggcggacacc tgaccaatgg gcatgatcag cgcgcgcatg gacgtgtgtt
    13681 cttctgcttc ttctttattt tttcaatttc acctgcttgt cttcccactg ccggactttg
    13741 agtcgtgcag catcgaacag ccgttcatag ttctgagcct ttactttgcg cgccgtctgc
    13801 ccatccagtc gttcccacaa tttctggtag gcctcgtatg ccttcaaata gtcggcttgc
    13861 gatttaggcg cgaccatgtc actgccgaac aggaagcgat cgggatactg ctgcagcagc
    13921 gtgacccatg catccaacgt tgcagcgtcg gccacgaccc acttggccac ctcatcccac
    13981 gagaggtcga aattcacgtg cgaatattct tcgccggaaa gcatctcacg caacaggttc
    14041 agatgatcgc tggtaggttt aacgaaacgg ccgagcccgg tgtgcgccca aataatcgag
    14101 gcgtcgcggt gcgaccgaaa tacgctcttg agatcatcga aatttgccgg ccgtcccggt
    14161 gccggccgca cagtattgat gtcgttgtgc aggatcacca cgagcccggt ctcgccggca
    14221 aaggtcaata tccggtccag cgccggattg agcaggctcg cggtatggcc tgcgactttc
    14281 gccgatacga attccttgtg gaccgagaat tctccgatgc cggaaaatac gcccggatac
    14341 atcagcagca cgcggcggat gtgatccacg gcatacatat cggtgggatt gaatccggtg
    14401 atcatcgggt caaaccgggc gcggtctttg gcagacaggt gcaaatactg atcggcaacg
    14461 atcgcatcgg tgaaagagta atagtaaagt tcggcatcgg tcagcaagta ataatccggt
    14521 gcgcgcgggc ctgactcgaa gtagtcccac ttctgctgca acggaatgcc gaatacggcg
    14581 acacggccgg cacgctcacc cataatgcgc aggaattcag gcaggctggg gccctcttgt
    14641 acgtaattgg tgaggtggaa gtgcgaatcg ttgaagtcgt gggccgccgc attgcttgcg
    14701 gaaccgcacg agagcagcgc aaaactcgca tatatcagca ggaaacgcag cttcttcatt
    14761 cgtgcagtcc tcgaaatgtg aacggcgcga gaatagcagc acgacaactg gcgctgactg
    14821 acgattttgt ttcatttgtt tcgtgatcct cacaaataac gcggccgaat atagtatggc
    14881 gaacaagtgc taagccccga atgagaccgc tgctattctc agcggctcac cccatcaacc
    14941 cgaataggag aaaagcaatg accaccgcga ctaaagagaa agcagtcaac aacggcgtaa
    15001 atgtcgaagc attgttcggt gcacgcgagg cgctcaccaa ggcacccgag gcggccaagt
    15061 tcaaatggcg cgcgtcttgc gaatgggtca aaggcacgca aagccgctcc accgtcgaag
    15121 gcttctatgg tctgggcgcg gaacagaagc acaaaacaaa attctcgttc cattccgatc
    15181 atccggaagt tttcgcgtcc gaggattccg gcgccacacc cgtcgagatc gtactggcct
    15241 ccctggctgg ctgcctcacg gccggcatcg cggccgtcgc gcaggcgcgt gacattcaac
    15301 tacgctcggt aaccgcaacg ctggaagcgg gcatggacat tcgcggtatc ctcggcgccg
    15361 accccgacgt gcgcaacggc ttcgacggtg tcaaggttac ctacaaaatc gatgccgacg
    15421 catccaaaga cgacatcaag gcgctggttg cgcaatcgca gaaacgttcg gccgtgtacg
    15481 acatcctcac caatccgacc aacgttactg tcgaagtgaa gtagccgatc ggagcaagcg
    15541 catgcgtacc acgacggttg taatcggcgc cgggcaggcc gggctggcca tgagttgttg
    15601 gctggcctgg cgcggcat
//
LOCUS       DATMZQ010000130         3554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_101749, whole genome shotgun sequence.
ACCESSION   DATMZQ010000130 DATMZQ010000000
VERSION     DATMZQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101749"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..825
                     /locus_tag="VN496_04085"
     CDS             241..825
                     /locus_tag="VN496_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF16157.1"
                     /translation="MVAVGVAFTHLSARAKEIRGVTKVFNQLLNSIAPESVESHISAN
                     HSRLYKSSMEGTLVKDLRILIVDDSETTRRILRAIIRSREWIICGEAETGRAGIRQFQ
                     KLKPDLVLIDLAMPDINGIEAARRMSILNPKTPLILFTILELEGIEKVAHEAGICAVV
                     SKAQAWDLVKSIETAVGYIQQHGQSSQEPGPQIQ"
     gene            complement(863..1978)
                     /locus_tag="VN496_04090"
     CDS             complement(863..1978)
                     /locus_tag="VN496_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF16158.1"
                     /translation="MSAPEKVNILMVDDQPGKLLSYEAILGELGENLIKANSGREALD
                     RLLKTDIAVVLMDVSMPELDGFELAGMIRQHPRFQHTAIIFISAVHLTDLDRLQGYQR
                     GAVDYISVPVIPELLRAKVSVFAELHRKTRQLETLNQDLRKLSSALIVTQDEERRRIA
                     RELHDGLGQELSAAKMMLDGIMRPDLSTEWKEKAAADSSTLIDRAIQQVRSMSHLLHP
                     PLLDEVGLCSALEQYLEGFTKRSGIETVVQLPPRDSPRFKPELETAIFRIIQEALTNV
                     FRHSGARKAWITVVTSESQTAVTVRDDGKGLDDSVIELRPGSVGIGLGGMRQRVKEFG
                     GELQLENANPGTQVKITIPANAMTVQAAVTHVGVQRS"
     gene            complement(2015..>3554)
                     /locus_tag="VN496_04095"
     CDS             complement(2015..>3554)
                     /locus_tag="VN496_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF16159.1"
                     /translation="AFEVSDTGIGIPVEKQRIIFEAFQQADAGTSRKYGGTGLGLAIS
                     RELAGLLGGEIQLRSAPGAGSAFTLYLPQTYVGSSAGVTVATDRRILQPASPLQLTSA
                     NVAEPVVEQVPDDRENLQPDDLILLIVEDDPHYARVLCDLAHDKGFKVLVAARGSEAL
                     MLVREFHPSAVSLDVFLPDMLGWTVLNHLKQEPATRHIPVQILTLDEDRQHGLSRGAF
                     SFVTKPTTREGLESAFSRIKEYATPRLKRLLVVEDDPAEQLSIRELLGYRDIQVSVAA
                     SGQEALNAINSEPFDCMVLDLRLPDMSGFDVLERLRDTPALNDLPVVVFTGKDLSSEE
                     DARLHTLARSVVVKGVESPERLLDETALFLHRVVSDLPAEKQKMLDRLHRSDDALIGK
                     KVLVVDDDVRNIFALSSVLERRGMVVMTAGTGREAIATLESTPGIAIVLMDIMMPEMD
                     GYETMQVMRQNPDFRRLPIIALTAKAMKGDREKCLEAGASEYLAKPVNTEQLLSALRM
                     WLHR"
BASE COUNT          742 a         1066 c          974 g          772 t
ORIGIN      
        1 gccaaaaggg ttattcaagg tttgcagggt cttttcctcc aaatcgatat aaccgagatc
       61 atagtccata aagctgacca gccagatacc gtcatgtgtg gactagacgc accggctgag
      121 tttgcatgag ccaccctaga gaatggctta atcaatcgtc tgtggtatgt accgctcgca
      181 cttcggcggc tcatgtcgta attggaggtg ctgaaatgca ggcgcagtcg attgaagaag
      241 gtggtggcgg ttggtgtcgc gttcacgcac ctgagcgcac gtgcgaaaga aatccgcggc
      301 gttacgaagg tatttaatca actgctaaat agtattgctc cagaatcggt agaaagccat
      361 atttccgcta atcactcacg gttgtataaa agttcgatgg aaggcaccct cgtcaaagac
      421 ctgaggattc tgatcgtgga tgactcggag accactcgcc ggattctccg cgccatcatt
      481 cgttctcgcg aatggatcat ttgcggtgag gcggagaccg gccgagcggg aattcgccag
      541 tttcagaagc tgaaacccga tttggtcttg atcgatctgg ccatgccaga catcaatggc
      601 atcgaagccg ccaggcgaat gtcaatcttg aatcccaaaa cccctcttat cctattcaca
      661 atattggagt tagagggaat tgaaaaggtg gctcacgaag ctggaatttg cgccgtcgtt
      721 tctaaagctc aggcttggga tttggttaaa agcatcgaga cagcggtcgg ctacatccag
      781 cagcatgggc agtcttcaca agagcctgga ccgcaaattc aatagtgagc acaaggcatt
      841 gccgtgcgtc gctgtcctgc ctctagcttc gttgtacgcc gacgtgcgta actgcggctt
      901 gaaccgtcat cgcatttgcg ggaatagtga tcttcacctg cgtccccggg tttgcattct
      961 ccagttgcag ctctccgcca aactccttca cccgttgacg catcccaccg agtcctatgc
     1021 caacgcttcc cgggcgcagc tcaatcacac tatcgtccaa ccccttgccg tcgtctcgca
     1081 ccgtcacggc agtctgcgat tctgacgtca cgacagtaat ccatgccttg cgtgcgcccg
     1141 agtgccggaa aacgttggtt agtgcttctt ggatgatccg aaagatggct gtctccagct
     1201 ccggtttgaa ccgcggggaa tcgcgcggcg gcaactgcac cactgtttca atcccgctgc
     1261 gcttcgtaaa gccttccaga tactgttcaa gcgcagagca cagccccact tcatcgagga
     1321 gcggcgggtg cagcagatgc gacatgctgc gcacctgctg aatcgcgcga tcgatgagcg
     1381 tgctgctgtc cgccgcggcc ttttctttcc actccgtcga caaatccggc cgcatgattc
     1441 catcgagcat catcttcgcc gcgctaagtt cctgaccaag gccatcgtgt aactccctgg
     1501 caatgcgccg gcgttcttca tcctgcgtga cgatcaacgc gctggatagc ttgcgcaagt
     1561 cctggttcag cgtctccagt tgccgcgtct tgcgatgtaa ctcggcaaag acgctgacct
     1621 tggcgcgcaa cagttcggga atcaccggca ccgagatgta atccacggcg ccgcgctgat
     1681 atccctgtag ccggtccagg tcggtgaggt gcactgcgga aatgaagatg atcgcggtgt
     1741 gctgaaaacg cggatgctgc cgaatcattc ccgcaagctc gaaaccatcc agttccggca
     1801 tgctcacatc catcagcacc accgcgatgt cggtcttcag caggcgatct agcgcttcgc
     1861 ggccggaatt tgccttaatc agattttcgc ccagctctcc aagaattgcc tcgtagctca
     1921 gcaactttcc tggttgatcg tcgaccatca ggatgtttac tttttcgggc gcgctcatcg
     1981 gcactctcgt cggatgcaag ggaagggcat aaagttatcg atgcagccac atgcgcaatg
     2041 ccgagagaag ctgctcggta ttcacaggct tggccagata ctccgatgcc ccggcttcca
     2101 ggcatttctc gcggtcgccc ttcatggctt tggcggtcaa ggcgatgatc ggcaaccgcc
     2161 ggaagtccgg attttgccgc atcacctgca tcgtttcgta gccgtccatc tccggcatca
     2221 tgatgtccat aagaacgatc gcgatgcccg gggtagattc cagagtagcg atggcttcgc
     2281 gtcccgtgcc cgccgtcatc accaccatgc cacggcgttc caggacgctg ctgagcgcga
     2341 aaatgttgcg gacatcgtcg tccacaacca ggaccttctt gccgatcaag gcgtcgtccg
     2401 agcgatggag ccggtctagc attttctgct tctcggcggg tagatcggag accacccggt
     2461 gaaggaatag cgcggtctcg tccaagagcc gctcgggtga ctccacccct ttgaccacca
     2521 cgctgcgtgc caaggtatgg agtcgcgcgt cctcttccga agacaaatcc ttgccggtga
     2581 aaactaccac cggcaagtca ttgagcgccg gcgtgtcgcg cagccgctcc agcacatcaa
     2641 atccgctcat gtccggcaac cgcagatcca ggaccatgca gtcgaatggc tccgagttga
     2701 tggcgttcag cgcttcttgt ccgctcgcgg ctacagaaac ctgaatgtcg cgatacccga
     2761 gcagttcgcg aatgctgagc tgctctgcgg gatcgtcttc cactactagc agacgcttca
     2821 agcgaggcgt cgcatattcc ttgatccgtg aaaaggcgct ctccagcccc tcgcgagtgg
     2881 tcggtttggt cacgaacgaa aatgcgccgc gcgacaatcc gtgctgacga tcttcgtcga
     2941 gggtcaggat ctgcacgggg atgtgtcgcg tagcaggttc ctgtttcaag tgattcagca
     3001 ctgtccatcc cagcatgtcc ggtaggaaaa cgtccaggga aacagcgctg ggatgaaatt
     3061 cgcgcaccag catcaacgcc tcgctgccgc gcgctgcgac cagtaccttg aatcccttgt
     3121 cgtgcgccag atcgcacaac acccgcgcat agtgcgggtc gtcttcgacg atcaacagaa
     3181 tcaggtcgtc gggctgcaga ttttcacgat cgtcaggcac ctgctccacc accggctcgg
     3241 cgacattcgc agaagtcagc tgcagcgggg aagcaggctg caagattcgc cgatccgtcg
     3301 caaccgtaac gccggctgag gagccaacgt aggtctgcgg caggtacagc gtgaacgcac
     3361 tccccgcgcc cggcgcgctg cgcagctgga tttcgccgcc aagcaaaccc gcaagctcgc
     3421 ggctgattgc caggcccaaa ccggtgccgc cgtacttgcg gctcgttccc gcatccgcct
     3481 gctggaaggc ctcgaagatg atgcgctgct tttcgacggg aataccgatt cccgtgtcgg
     3541 agacttcgaa cgcg
//
LOCUS       DATMZQ010000131        10986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2866756, whole genome shotgun sequence.
ACCESSION   DATMZQ010000131 DATMZQ010000000
VERSION     DATMZQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2866756"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(462..752)
                     /locus_tag="VN496_04100"
     CDS             complement(462..752)
                     /locus_tag="VN496_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF16160.1"
                     /translation="MTKARPQAASKLLQETGRAIVTEWRFAPGAETGWHRHAHDYIVV
                     VLTAGKLMQETSNGEIVTDLAAGQSYARDAGVEHNVVNPNPHEFVFVEVELK"
     gene            complement(787..2022)
                     /locus_tag="VN496_04105"
     CDS             complement(787..2022)
                     /locus_tag="VN496_04105"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXF16161.1"
                     /translation="MVNSVAPTRDGLLDGYTVIDFTRVLAGPYCTRLLADLGARVIKI
                     ERPGEGDEIRYTAFQLEADRADQSSYFARLNTGKEGIAINLARPEARELVLDLIRNAD
                     VVVENFSPGVMARYGLDAAALRGLFPDLVYCSISGFGQTGPLSSLQAYAHLINAISGL
                     MDLDRGGETQPRVSYLQAADVLAGTHAFGAICAALLRRARSGEGATLDISMLECLIAA
                     DDITYGALLNGGPVERQPRIGMMVHAFGERHIALQTAGAPHLWSRLVALVGKPELAAD
                     PRFVTPQARRKNWGALQQVYREWLNRYQDADEAIEALSAARVPVVPMLSPEEVVNHPQ
                     LLSREAFPRVPHAGCGSVRVTSTPFFVDGKPTHPRAGAPYLIGEHTRDVLATVLGYST
                     QKIDGLQKLGVIEMPEPLQ"
     gene            2585..4366
                     /locus_tag="VN496_04110"
     CDS             2585..4366
                     /locus_tag="VN496_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16162.1"
                     /translation="MTGRARWNRCRARSPCRCDGSPQAATRIKATSASRKLRDIPSVR
                     HEVARPLRRFVVTLALLAGDCGMGWATEMRAGEALPSFAELQDKGAVVGKISIDNQNI
                     FDLEDPEENNFVFRTANTLHIKTRPEVIAQSLLFRSGDPVSVRLIEESERVLRCNASV
                     YHVSIQPEAYHDGVVDISVTTRDTWTLEPGIAFSRAGGSNSTGVSLEESNLFGTGIAV
                     GMAYTSTVDRAGTDFSFGYNHLFDGWTQIAYSIGSLDDGERQAFKLVRPFYSLDSRWS
                     AGVLGTKDDRIESTYQAAQIVNQYRYRQESAEVFGGLSSGLVGRWTQRYSVGLSYLDE
                     KFTRDPFLVSPATLPPDQTLVSPFVRYELVEDDFKIMTNHKQIELAESVAMGLHAQAQ
                     IGRSLSELGSSRELWLYSARAEKGFQIRSKDTLMTSASLSGQYGDGRVERQFLGGAAE
                     YYRPHAGRAVFYSSISGGVLRNPQVNDMLMLGGDNGLRGYPLRYQNGTQQALFTLEER
                     VYTDWYPFRLFRVGGAVFFDVGRAWGGDFQNTANPGWLSDVGFGLRILSCRSAFGNVL
                     HADIAFPLNGDSDIRSVQFLLRSKATF"
     gene            complement(4490..5329)
                     /gene="hflK"
                     /locus_tag="VN496_04115"
     CDS             complement(4490..5329)
                     /gene="hflK"
                     /locus_tag="VN496_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXF16163.1"
                     /translation="MNADEHGVVRRFGAIAARVGPGMHYRMPWPIDRVDVLKTTSVMK
                     IGVGFALRDGETAAPAGVELLTGDTNILNVALVVQYAILNPADYLFQIDRPQTLVSTL
                     AESVLTETVLGMGIDEVLTTGRLAIQARVKTKTQETLDRYRSGIRIASVSIMNISLDS
                     SVAQSFQDVADAMADREKAQNQARAYANDQIPRARGEASQTLSSAKSYKQQRIAEAIG
                     NTTRFLALLNEYQKAPEVTRSRLYLDSMEKILPKVKLYVIDSQGGRVPINLRVTTPGG
                     IEK"
     gene            complement(5440..6480)
                     /locus_tag="VN496_04120"
     CDS             complement(5440..6480)
                     /locus_tag="VN496_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXF16164.1"
                     /translation="MDAASALIANPARRWQAVAATIAVMAIAVWAALSSLFVVDVTQY
                     ALVTRFGAVLRVLDEPGLQVKAPFDSIVTLDKSLTFSRPAPAEYLSVDKKNIVAETLV
                     TWRIADPQRYLVTVATRAAADVRLADVLLGEVGAVLGAHPAAALIAPDGNAERFGSIV
                     AQIRGRVAAFARSAYGIEIVDVELVRLSLPEQNRAPVFERMKAERGKMAKEYRTAGEL
                     LGRKIIAQADREKAHIEAEAYAEAQRIRAEGDAEATRLYSAAFSRNAGFYKFVRTLQT
                     YEKIMDENTTLFLPAQSEVLRVIAPLPRQGSSTPAQPMESTTASGKGPLRSPGADPTP
                     VAGAPSTRRALQ"
     gene            complement(6482..8638)
                     /locus_tag="VN496_04125"
     CDS             complement(6482..8638)
                     /locus_tag="VN496_04125"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HXF16165.1"
                     /translation="MTEFLLRWLWETWIIFKQAAVFLLFGFFIAGLLAVCVPEKLLSR
                     LFRNGRIKSVLWAATVGAPLPLCSCGVVPAALALKRQGATPGATVAFLIATPETGADS
                     ISLTYALMDPLVTVFRPVAAVVSALTAGLLTNLFGERKRAEEQTVECASDCHAPHAHE
                     PNDHVHEPIAAPAPDAWPASRAARLWRILQRVNHYAFRQLLDDIGYWIVLGIVLSGLI
                     AAALPPAFFERYLGSELVSMLVMLLVGVPIYVCASEATPLAAALVLKGLNPGAALVFL
                     LVGPATNIGSLVPLTRFLGARIMAIYLGAIVGVALLAGFALNAIYRAWGVDPRATFGT
                     ATGLLPDWLKVAGALLLLVLLVLSMRRAPVPVEWIWLRDRFARMSGLRVTAAKLAGIA
                     AALAAVLYAASGFIAVQPGEVGLRTRFGEIVAPVLEPGLHYRMPWPFEAHRIVRKDEV
                     QRIEFGFAGGKAPAAPAIRTTSAAGWNPLIDGGNSGGAWFQKEAGPEEPFLLTGDGNL
                     IDLRWAVQFRVKDAVAYAYRVAEPEALVRSVTLAALRSVAARNSIDAIYTADRSSVEQ
                     NVEQSLQQTLDETGSGIEVVSFQLLYVHPPGEVHDAFRDVASSQEDKLRTINRANIFA
                     VEMVNQAKADAAAMIEQALAFKEQQILHAQGDADNFTLKLGAYRRAPELTEFRLQLES
                     IEETLPGLQKFIMPDARDVKDFDMWLLQPAALGRGK"
     gene            complement(8723..9748)
                     /locus_tag="VN496_04130"
     CDS             complement(8723..9748)
                     /locus_tag="VN496_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXF16166.1"
                     /translation="MFSVHSSLARAWLLAMVGVFVQGGAAVAAEHKYDPGNDVKFTYC
                     YAHQPVLRIKPGDSVVTSTRDASNDVYGTGDKTLFPKLDLTKVNPQTGPFYIEGAEPG
                     DTLVVHLDKIDFNREFGWGGSIPYFGALAPEYKTMMITPPVPDRLFVWKIDRSRRVAT
                     LDLPNSKIGKVEVALRPFFGTIGTAPGGKECISSLVPGQHGANMDFNETVEGTTLYFP
                     VNEPGALFMLGDGHAAQGDGEIDGAAIETSFNVRFTVNLIKGKKIGWPRLVNDKVAMS
                     IGSTRPLMDALRLACSDLIGWMVEDYGYDKYEALQLLGQVAQIKIANVVDPQYSVACI
                     IDKKYLK"
     gene            complement(9819..10787)
                     /locus_tag="VN496_04135"
     CDS             complement(9819..10787)
                     /locus_tag="VN496_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXF16167.1"
                     /translation="MAETDDRGELPAHRRWQHVYIVLGFACALAAVAKLSCFIVETGE
                     HALVTEFGKPVQVITSPGLRFKIPYQSVRTFDSRLFVYTPAATEFLTVEKTPVVAAST
                     ILWRIADPEKFFQTVFDRSGAESRLGDILFAELGAAIGRNSLSAFFSMEPSSFKSEAV
                     LAEVSRKCREIALRDFGIGINGVLLRNFDFPKQNRPPLYSRMKSERGRLSMKFRSEGE
                     EEGLKVRAAADQEKSRILSEALKQAQQTRSEGEGRAARIYAESLAKGPDFYTFVRTMQ
                     ASRNLVPKGTTLMLPADSELFGLLQDSSHYDRNRANRKTSAGRTSK"
BASE COUNT         2106 a         3400 c         3256 g         2224 t
ORIGIN      
        1 gccgtgacac ggctagtcgc tttcttctag tctggtatgc gcggctccat cgggcgagct
       61 gtgcgctata accatcacgt attattgaac gttaatatcg ttttcgactg ccttcacgcc
      121 ctggataccg cgcgcaatct ctgccgccct ctgcgactct ttcccggctg gcagcgctgc
      181 cactcagcca ggttcgcgca cgttcagcag tcatcgtttc cctcccgtgg gttaggaccg
      241 gcgccgccgt acggaatcgc cattggtgga tgccggtcag tggctcttgg acgccgcttg
      301 gaccgccttg gttccggaaa gcagctgtcc gcgttgcgcg cggacgctgg cccgcggctt
      361 tcaaacaatc ccaggatgcc cctcctgggt agcaatgctg caggcgttgc tgcggcactg
      421 acttgccgtg taatcagaat tctctgctgc ttttctctcg attacttgag ttcaacttcc
      481 acaaaaacga actcgtgtgg gtttggattc acgacattgt gctcgacgcc ggcgtcgcgc
      541 gcataggact ggccggctgc cagatcggtc acgatctcgc cattggatgt ttcctgcatc
      601 agcttgccgg ctgtcagcac aaccacgatg tagtcatgcg cgtgccggtg ccaacctgtc
      661 tccgcaccgg gcgcgaagcg ccattccgtc acaatcgccc ggccggtttc ctgcaaaagc
      721 ttggaagcag cttgtggtcg cgctttggtc atgaattctc ctttgctgaa gatgtcatgg
      781 gcagcatcac tgcagcggct ctggcatttc aatgacgcca agcttttgca agccgtcgat
      841 cttttgagtt gaatagccca acacggttgc caggacatct cgtgtatgtt cgccgatcag
      901 atatggcgcg ccggcgcgcg gatgcgtggg cttgccgtca acaaaaaacg gcgtagacgt
      961 tacgcgaacg ctgccgcagc cggcatgcgg cactcgcgga aaggcttctc gcgacaacag
     1021 ttgcggatga ttgacgactt cttctggcga cagcatgggc actaccggca cgcgcgccgc
     1081 agacaatgct tcgatggcct cgtccgcatc ctggtatcga ttgagccatt cgcgatacac
     1141 ctgctgcaac gcgccccaat ttttgcgtcg tgcctgtggc gtgacgaagc gcgggtccgc
     1201 cgcgagttcc ggtttgccga ccagtgcaac caggcgcgac cacagatgtg gagcgcccgc
     1261 cgtctgcagc gcgatgtgcc gctcgccaaa agcatgcacc atcatgccaa tgcgcggctg
     1321 gcgttccacc gggccgccgt ttagcaatgc gccataggta atatcgtcgg ccgcaatgag
     1381 acattcgagc atggagatat cgagcgttgc accttcgccg ctgcgagccc gcctcagcaa
     1441 ggcggcacag atggcgccga aggcgtgggt tccggcgagc acatccgcgg cttgcaaata
     1501 agaaacccgc ggctgcgttt ctccgccgcg atcgagatcc atcaggccgg agattgcgtt
     1561 gatcagatgc gcgtacgcct gcaatgagct gagcgggccc gtctggccga aacctgaaat
     1621 ggagcagtac accaggtcgg gaaacagacc gcgcagtgcg gcggcgtcca ggccgtagcg
     1681 cgccatgaca cccggcgaaa aattttcaac taccacgtcg gcattgcgta ttaggtcgag
     1741 gaccagttcg cgcgcctccg ggcgggcgag attgatcgcg atgccttcct tgcccgtgtt
     1801 caggcgcgcg aagtagctgc tttggtctgc acgatctgcc tcgagctgga aggccgtata
     1861 gcgaatttca tcgccttcgc cgggacgctc tatcttgatg acgcgcgcgc caaggtctgc
     1921 aagcaggcgc gtgcagtaag gtcctgccag cacgcgggta aagtcgatga cggtatagcc
     1981 atcgagcaga ccgtcacgcg tgggcgcaac agaattcacc attcaaagcg gcataagtta
     2041 gtcaatctat gaaggcgttg ccggtgagcc gcccgttagt ggtcccccgg ctcgttcgag
     2101 cgcgattcta tccgctatcc ggtacttcaa tatgaaaact gtgcgccagc agaaacggtc
     2161 gagtgtcacc tgttgtgtcg agggtttgcc ttggccgtcg caaagcagcg acgtcgcttt
     2221 ctacaaagcc ggctgggaaa ttttacaagc agcgcactgc gcaccagtaa attgtgcaaa
     2281 cgagcgagcc gcttgcggca gacgaacgtc aacgtgcgcc attcgtgacg gcgcagcgtc
     2341 ggatattctc tacagagaac ggcgtgcgac gtacaaatgc tgcctgcggt gcgtgcgtcc
     2401 gcttgcccag cattacacgt taagcacgtc acgcacagct aaacggatta ctgccaatgg
     2461 catagctgct gcatggtatt ggcataactg cctgccgcga aaaatcgatg tgtcagtttt
     2521 gcaaagtgct cctgtttccc acatcccttt gtcgcgcagg cagaccgcct tatcatctat
     2581 tcgattgacc ggacgcgcgc ggtggaaccg atgccgcgcg cgctcgccgt gccgctgtga
     2641 tggcagtccg caagccgcta cgcgaataaa ggctacgtcc gcctcgcgca agctgcggga
     2701 tattcccagc gtacgtcacg aagtggcccg gccattgcgt cgctttgtgg tgacgcttgc
     2761 cttgctcgcc ggggactgcg gcatgggatg ggcaactgaa atgcgcgccg gcgaagcact
     2821 accctcgttt gccgaattgc aggacaaagg cgcagtcgtg ggcaaaatca gcatagacaa
     2881 ccagaacatt ttcgatctgg aagatcccga agaaaacaat ttcgttttcc ggacggcgaa
     2941 tacgctgcac atcaagaccc ggccggaagt cattgcacag tcgctgctgt tccgtagtgg
     3001 cgatccggtt tccgtgcggc tgatcgaaga aagcgagcgc gtgcttcgct gcaatgccag
     3061 cgtgtaccac gtgagcatcc agcccgaggc gtatcacgac ggcgtagtgg atatctcggt
     3121 cacgactcgc gacacctgga cactggaacc cggcattgcc ttcagccggg ctggcggttc
     3181 caattcaact ggcgtctcgc ttgaagagag caaccttttt ggcaccggca ttgccgttgg
     3241 catggcttat acctcaacgg tggatcgcgc cggcacggac ttcagcttcg gttacaacca
     3301 tctcttcgat ggctggacgc agatcgccta ttcgatcggc tcgctggacg atggggagcg
     3361 gcaggcgttc aagctggtgc ggccctttta ttcgctcgat tctcgctggt ccgccggcgt
     3421 gctcggcacg aaagacgatc gcatcgaatc gacctaccag gccgcgcaga tcgtcaacca
     3481 gtaccgctac cgccaggaat cggccgaagt gtttggcggt ttgtccagcg ggctggtcgg
     3541 acgctggacg cagcgctact ccgtaggtct gagctatctg gacgaaaaat tcacgcgcga
     3601 tccgttcctg gtgtcaccgg cgactttgcc accggaccag acgctggtgt cccctttcgt
     3661 gcgctatgaa ttggtcgaag atgacttcaa gatcatgacc aatcacaagc agattgaact
     3721 tgccgaatcg gtcgcgatgg gactccacgc gcaggcacag ataggccggt cgctgagcga
     3781 actcggctcc tcacgagaac tctggctcta ttcggccaga gcggaaaaag gatttcagat
     3841 ccggtccaag gataccttga tgacatctgc atcactttcc ggacagtacg gagatggtcg
     3901 cgtggagcga caatttctgg gaggagccgc ggaatattac cgtccgcatg caggtcgcgc
     3961 agtcttttac tcttcgatat ccggcggcgt tctgcgcaat ccacaggtca acgacatgct
     4021 gatgcttggc ggcgacaatg gcctgcgcgg ttacccgctg cgctatcaga acggcaccca
     4081 gcaagcgttg ttcacgcttg aagaacgcgt ctacacagac tggtatccgt ttcgcctgtt
     4141 ccgtgtcggc ggcgctgtgt tcttcgacgt tggccgcgcc tggggcggcg atttccagaa
     4201 cacggccaat cccggctggc tcagcgacgt gggtttcggc ctgcggatat tgagctgtcg
     4261 ctccgcgttc ggaaatgtgc tgcacgcgga catcgcgttt ccgctgaacg gggattcaga
     4321 catcaggtcg gttcagttcc tgttgcgctc gaaggcaacg ttctgaagtt ttgggcgtca
     4381 gcgtttcaga gtttagggct gctcgcttgt tcttccccat ctgtagcacc ccataaggct
     4441 caggcagccc gatcccgaaa tcgggtgcat cggaggtgat gcctataacc tatttctcaa
     4501 tgcccccggg cgtcgtgacg cgcagattga tcggcacgcg cccgccctgc gaatcgatga
     4561 catacagttt caccttgggc aggatctttt ccatcgagtc gaggtagagc cgggagcggg
     4621 tcacctccgg cgctttctga tattcgttca gcagcgccag gaaacgggtg gtgttgccga
     4681 tcgcctcggc gatgcgctgc tgcttgtagc ttttggcgct gctcagcgtc tgactggctt
     4741 cgccgcgcgc acgtggaatc tggtcgttgg cataggcacg tgcctggttc tgcgcttttt
     4801 cgcgatccgc catggcatcg gccacgtcct gaaaagactg tgccaccgag gaatccagcg
     4861 agatgttcat gatgctgacc gaggcgatgc gaatgccgct gcgatagcgg tccagcgttt
     4921 cctgcgtctt ggtcttgacg cgcgcctgga tcgccagccg gccggtggtc aatacctcat
     4981 cgatgcccat gccgagcacg gtttcggtca gcacgctttc cgccaaagtc gacaccagtg
     5041 tctgcggacg gtcgatctgg aacaggtaat ccgcgggatt gagaatggcg tactgaacca
     5101 ccaaggcaac attcagaatg ttggtgtcgc cggtgagcaa ttccacgccg gccggtgccg
     5161 cggtttcgcc atcacgcagc gcaaatccca cgccgatctt catgacactg gtggtcttca
     5221 ggacatcgac gcggtcgatg ggccagggca tgcggtaatg catgccggga ccgacgcgcg
     5281 cggcgatcgc gccgaacctg cgcaccacgc catgctcgtc ggcgttcacc gtgtaaaaac
     5341 cggaagcgaa atatgcagtg acaaggccca ccgcgatcag gacggcccaa tagcgccgcc
     5401 gaactttcgc cggcgcgagg ctgtaggacg catccgcgtt cattgcagtg cccgtctagt
     5461 cgacggggcg ccagcgaccg gcgtgggatc tgcgccggga ctacgcagtg gtcctttgcc
     5521 gctagcggtg gtcgattcca ttggctgagc aggagttgac gaaccctggc gcggcagcgg
     5581 cgcgatgaca cgcagtacct ccgactgcgc gggcaggaac agcgtcgtgt tttcatccat
     5641 tattttttcg tacgtctgca gcgtgcgcac gaatttgtag aacccggcgt tgcgactgaa
     5701 cgctgccgag tacagccgcg tggcctcggc gtcgccctct gcccggatgc gctgggcctc
     5761 tgcgtaggct tccgcttcga tatgggcttt ctcgcgatcg gcttgcgcga tgatcttgcg
     5821 gccaagcagt tcaccggccg tgcggtactc cttggccatc ttgccgcgct cggccttcat
     5881 gcgctcgaaa acgggcgcgc gattctgttc cggcaagctc aggcgcacca gttcgacgtc
     5941 gacgatttca atgccatagg cgctgcgtgc aaaagcagcc acgcgcccgc gaatctgcgc
     6001 cacgatgctg ccgaagcgct cggcattgcc atccggtgcg atcagcgccg cggccggatg
     6061 ggcgcccagc accgcgccga cttctccaag caacacgtcc gccagccgca cgtccgccgc
     6121 agcacgggtt gcgaccgtaa ccagatagcg ctgcgggtcc gcgatgcgcc atgtcaccag
     6181 cgtctccgcc acaatgtttt tcttgtcgac gctcagatac tccgcgggcg ccggacgcga
     6241 gaacgtcaag cttttgtcga gcgtcacgat gctgtcgaac ggcgctttga cctgcaggcc
     6301 cggctcgtcg agcacgcgca acaccgcacc gaagcgagtg accagcgcgt actgcgtcac
     6361 atcgaccaca aacagactgg aaagcgctgc ccacaccgcg attgccatca cggcaattgt
     6421 tgccgccaca gcttgccaac gccgcgccgg attggcgatg agggcgctgg ccgcgtccac
     6481 tttatttgcc tcgtccgagg gcggccggct gcagcagcca catatcaaaa tccttgacgt
     6541 cgcgagcatc gggcatgatg aatttttgca gccccggcag cgtttcctcg atgctctcca
     6601 gctgcaggcg gaattcggtg agttccggcg cgcgccggta ggcgccaagc tttagcgtaa
     6661 agttatcggc atcgccttgc gcatgcagta tctgctgctc cttgaatgcc agcgcctgct
     6721 cgatcatggc agctgcgtca gctttggcct ggttcaccat ctccaccgca aagatgttgg
     6781 cacggttgat ggtacgcagc ttgtcttcct gcgaacttgc cacatcgcgg aacgcatcgt
     6841 gtacttctcc cggcggatgc acgtacaaca gctggaagga aacgacttcg atgccgctcc
     6901 cggtttcatc gagtgtttgc tgcagtgatt gctcaacgtt ttgctccaca ctgctgcgat
     6961 cagcggtata gatggcatcg atgctgttgc gcgcggctac gctgcgcaaa gccgctagcg
     7021 ttacgctgcg caccagcgcc tcgggctctg cgacacggta ggcgtacgcc actgcgtcct
     7081 tgacgcggaa ctgcaccgcc cagcgcaaat cgatgaggtt gccgtcaccc gtcagcagga
     7141 acggctcctc cggacccgcc tccttctgga accacgcacc gccagagttt ccgccgtcga
     7201 tcaacggatt ccagccggcg gcgctggtgg tacggattgc tggcgccgct ggtgccttac
     7261 cgcccgcgaa cccgaactcg atgcgctgaa cctcgtcctt gcggacgatg cgatgtgctt
     7321 caaatggcca tggcatccgg taatgcagtc ccggctcgag tacaggcgcc acgatttctc
     7381 cgaagcgtgt gcgcaatccg acttcacccg gctgcacggc gatgaagcca ctcgctgcat
     7441 agagcacggc cgcaagcgcc gccgcgattc cggccagttt tgccgccgtc acgcgcaatc
     7501 cggacatgcg cgcaaagcgg tcgcgcagcc agatccattc aaccggcacc ggtgcgcgcc
     7561 gcatgctgag cacaagcagc accaggagca gcagcgcacc tgccactttc agccaatcgg
     7621 gaagcaagcc ggtggcggtg ccgaacgtcg cgcgcgggtc gacaccccac gcgcggtaaa
     7681 ttgcattcaa cgcgaagccg gccagcagcg caacgcccac gatcgcgccg agataaattg
     7741 ccatgatcct ggcgcccaga aaccgcgtca gcggcacgag gctgccgatg ttggtagccg
     7801 ggccgaccag cagaaacacc agtgcagcgc ctggattcag gcccttcagc accagcgcgg
     7861 cggccagtgg cgtcgcctcg gacgcacaca cataaatcgg cacgccgacc agcagcatta
     7921 ccagcatgga gaccagttca ctgccgagat aacgctcgaa aaaagcgggt ggcagggcag
     7981 ccgcaatgag cccggacagt acgatgccca gcacgatcca gtaaccgata tcgtccagca
     8041 actggcggaa cgcgtaatga ttgacacgct ggaggatgcg ccataatcgg gccgctcggc
     8101 tggctggcca cgcatccggc gccggcgcag cgatcggttc atgcacatga tcgttgggtt
     8161 cgtgagcatg cggggcatgg caatcgcttg cgcactccac cgtctgctct tcagcgcgct
     8221 tgcgctcgcc gaacagattg gtgagcagac cggctgtgag cgcggaaacc actgcagcga
     8281 ccggacggaa caccgtgacg agcggatcca tcagcgcata cgtcaggctg atggaatcgg
     8341 ctccggtttc cggcgtcgca atcagaaagg cgacggtagc gccaggcgtt gcgccctggc
     8401 gtttcaaggc tagcgcggcc ggcaccacgc cgcacgagca cagcggcaaa ggcgccccga
     8461 cagtcgcggc ccacagtacc gatttgattc tgccgttgcg gaacagtctg gacagcagct
     8521 tttccgggac acacaccgcc aacaggccgg caataaagaa accgaacagc aaaaatacgg
     8581 cggcctgctt gaaaataatc caggtctccc acaaccaccg cagcagaaat tcggtcatgg
     8641 cttatttcca tcagacgcga acaagcggcg gctcgatgcc gccaccgcct gcccgcttgt
     8701 cggcgcctgt cttatgcgaa cctcacttca aatatttctt gtcgatgatg caggcgaccg
     8761 aatactgcgg atccaccaca ttggcgatct tgatctgagc gacctggccc agcagctgca
     8821 gcgcttcgta tttatcgtag ccgtagtcct cgaccatcca gccgatcaga tcagagcaag
     8881 ccaggcgcaa ggcgtccatc aggggccggg tgctgccaat cgacatcgcg actttgtcgt
     8941 tgaccagccg cggccaaccg atttttttgc ctttgatcag gttgacggtg aagcgaacgt
     9001 tgaacgaggt ttcaatggca gcgccgtcga tctccccgtc gccctgtgcc gcatgaccgt
     9061 cgcccagcat gaacagtgca cccggctcgt tgaccggaaa gtacaacgtc gttccttcca
     9121 ccgtttcgtt gaagtccatg ttggcgccgt gctgcccggg aaccagtgaa ctgatgcatt
     9181 ccttaccccc tggcgcggtg ccgatggtgc cgaagaaggg acgcagggct acttcgactt
     9241 tgccgatctt gctattgggc agatccagcg tagccacgcg gcggctgcgg tcgattttcc
     9301 acacgaacag ccgatcgggc accgggggcg tgatcatcat ggttttgtat tccggcgcca
     9361 gcgcgccgaa atacggaatc gagccgcccc agccaaactc gcggttgaag tcgattttgt
     9421 cgaggtgcac caccagggtg tcgccgggct cggcgccttc gatgtagaaa gggccggttt
     9481 gcggattgac cttggtaagg tccaatttcg gaaacagcgt cttgtcgccc gtgccgtaga
     9541 cgtcattgga agcgtcgcgc gtgctggtca ccacgctgtc tcccggtttg atgcgcagca
     9601 ctggctggtg cgcataacaa taagtgaatt tgacgtcatt gcccggatcg tatttgtgtt
     9661 ccgcggcgac agccgctccg ccctggacga aaacccccac cattgcgagc aaccacgctc
     9721 gcgccaaact gctgtggact gaaaacatga cccctcctcc tcgttggtgc tgctggttat
     9781 cgaatccggt gaatggacaa ttgccctctc gccgtgccct acttgctggt ccgcccggcg
     9841 ctggttttgc ggttcgcccg gttacggtcg taatggctgc tgtcctgaag cagaccgaac
     9901 aactccgagt cggccggcag catcagggtg gttccttttg gtacgagatt gcgcgacgcc
     9961 tgcatggtgc gcacgaaggt gtaaaagtcc ggccctttgg cgagtgattc cgcatagatc
    10021 cgcgccgccc ttccttcgcc ttcgctgcgc gtctgctgcg cctgcttgag cgcttcggac
    10081 agaatgcggc ttttctcctg gtccgccgcc gcccgcacct tgaggccttc ttcttcacct
    10141 tccgaccgga atttcatgct aaggcggcca cgctccgact tcatgcggga atacagcggc
    10201 ggccggtttt gcttggggaa gtcgaaattt cgcagcaaca cgccattgat gccgatgcca
    10261 aaatcgcgca acgcgatctc gcggcacttg cggctgactt cagcaagaac cgcttcggac
    10321 ttgaaggacg atggttccat ggaaaaaaaa gcgctcaatg aattgcgccc gatggccgcg
    10381 cccagttctg cgaacaggat gtcgcccagc ctggactccg cgccactgcg gtcgaacacg
    10441 gtctgaaaaa atttctcggg atcggcgatg cgccagagaa tggtgctggc ggccacgacc
    10501 ggggtttttt caacagtcag gaattcagtg gcagccggcg tatagacgaa caggcggctg
    10561 tcgaaggtgc gcacgctttg gtacggaatt ttgaagcgca ggcccggact ggtgatgacc
    10621 tgcaccggct tgccgaattc cgtaacgagc gcatgctcgc cggtttcaac gatgaagcag
    10681 ctgagttttg caacagctgc cagcgcgcag gcgaacccga gcacgatgta aacgtgctgc
    10741 caccggcggt gcgccggcag ttcgcccctg tcatcggttt ccgccatgac ctccccccgg
    10801 tcaactcagc gcgcgacatt tgttgggact tgccgctaga gtctgcggcg attgcaggaa
    10861 gtttacaagc gccactcaat gcataatgtg ctgcgccgca atgcccggcg catgtgcgta
    10921 gtgcgaaacg cctgaggggt ctgctacagt gcctgcctct taatccccgg tagccgcatg
    10981 cccacc
//
LOCUS       DATMZQ010000132        10285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_105362, whole genome shotgun sequence.
ACCESSION   DATMZQ010000132 DATMZQ010000000
VERSION     DATMZQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105362"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1529)
                     /gene="hflX"
                     /locus_tag="VN496_04140"
     CDS             complement(45..1529)
                     /gene="hflX"
                     /locus_tag="VN496_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXF16168.1"
                     /translation="MGVRRPVAPAKYRSTLEESNRPMGPEGREKSLRAVVAAVQLTGV
                     SDIEFNSSVNELRELAKTLGFELVGTFTQRRDHFDTTAYMGVGKRQEMRRFVENEPET
                     EQRTHESVASGNARPGGKSGAARAKEAAKSEADPETRCADIVLVDHEISPSQARNLEI
                     EVGCEVMDRTMVILEIFHRHARSRAARAQVEIARLGYMAPRLREQAKLAGPKGRQRSG
                     TGGRGAGEAHSALDRQKIRDRIAELQEEIKAMDAERKTQRARRHEHQGLARVVLVGYT
                     NAGKSTLMRALTGSEVLVANKLFATLDTTVRALHPDSVPRVLISDTVGFIKNLPHDLV
                     ASFKSTLEEAAEASLLLHVIDASDPGFERQLAVTDDVLNEIGAQRAPRIRVFNKIDCV
                     GDAAAQASQETALRQRYPDCMVMSARREADIATLHSSIRAFFQKDLIEAELFLPWAAQ
                     QLRGEIFATCEVMDERTEGAGTLFRVRAAPDTVNRMREEASKSI"
     gene            complement(1520..3085)
                     /gene="guaA"
                     /locus_tag="VN496_04145"
     CDS             complement(1520..3085)
                     /gene="guaA"
                     /locus_tag="VN496_04145"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXF16169.1"
                     /translation="MTHDKILILDFGAQYSQLIARRVREAGVYCELHPYDVSDEFVRG
                     FAPKGIILSGGPASVVVDGTPRAPQTVFDLGVPVLGICYGMQTMAAQLGGVVENGLIR
                     EFGYAEVRARGHSRLLDGLEDRRNAEGHGLLDVWMSHGDKVIALPPGFQIIASNAATP
                     IAGMADESRRFYGVQFHPEVTHTLQGKALIQQFLHRVCGLRSEWNMPDYLEEAVGRIR
                     SEVGNDEVILGLSGGVDSSVAAALIHRAIGTQLTCVFVDTGLLRLNEAVQVMETFAGN
                     LGVRVIAVDASEQFMSALKGISDPEQKRKIIGREFVEVFQREAARLPNARWLAQGTIY
                     PDVIESAGAKTKKAHTIKSHHNVGGLPETLHLKLLEPLRELFKDEVRELGLALGLPHD
                     MVFRHPFPGPGLGVRILGEIKSEFTELLRRADAIFIEELRASGWYEKTAQAFAVFLPV
                     RSVGVMGDGRTYEYVVALRAVQTDDFMTAHWAELPYTLLSRVSNRIINEVRGINRVVY
                     DIASKPPATIEWE"
     gene            complement(3168..4628)
                     /gene="guaB"
                     /locus_tag="VN496_04150"
     CDS             complement(3168..4628)
                     /gene="guaB"
                     /locus_tag="VN496_04150"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXF16170.1"
                     /translation="MRVLQKALTFDDVLLVPAHSTVLPRDVSLTTQLTSSISLNIPLV
                     SAAMDTVTEARLAIAMAQEGGIGIVHKNMLPKAQAAEVAKVKRFESGVVKDPITISQD
                     MTVRNVLNLIRQHRISGLPVVDGTKVVGIVTNRDLRFESNLDQPVKNIMTPRDRLVTV
                     REGVSIEDAMRLLHQHRLERVLVINEKFELRGLITVKDILKSSEHPYASKDQLGRLRV
                     GAAVGVGEGTEERVGFLVEAGVDVLIVDTAHGHSQGVLDRVRWIKRTYPAAQVIGGNI
                     ATAAAAKSLLDQGADGVKVGIGPGSICTTRIVAGVGVPQITAISEVAAALKGTGVPMI
                     ADGGIRYSGDIAKAISAGADSVMLGGLFAGTEEAPGEIELFQGRSYKSYRGMGSLGAM
                     QQGSSDRYFQDDEDAADKLVPEGVEGRVPYKGSVVQVMHQLMGGLRSAMGYLGCPTIE
                     TVKQRAEFVQITTAGVRESHVHDVQIVKEAPNYHVE"
     gene            4856..5356
                     /locus_tag="VN496_04155"
     CDS             4856..5356
                     /locus_tag="VN496_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXF16171.1"
                     /translation="MSCPLGNTALLLAIVVAATSGAAIGAQTYKWTDAEGKVHYSDQP
                     PPANIKEPTTVKTPRQSAPAAPSAPNAKGAPPPKAKTYTEQEADFKKRQVETAEREAA
                     EKKKADEAAERKQTCTQVRAQLKGLQAGGRLTQTNAQGEREYMSDAQIGQEIDRLKKA
                     EAGVCN"
     gene            complement(5369..5647)
                     /locus_tag="VN496_04160"
     CDS             complement(5369..5647)
                     /locus_tag="VN496_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HXF16172.1"
                     /translation="MKIRVEVVYAQPHTQEVIVLELEQGARVADALAATGLSGRQTAR
                     NYVGIWGALVSPETELRDRDRIEIYRELVADPKHARRQRARQQRKPRR"
     gene            complement(5644..6081)
                     /locus_tag="VN496_04165"
     CDS             complement(5644..6081)
                     /locus_tag="VN496_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HXF16173.1"
                     /translation="MALVEKSVLIEYSAAQMYALVENIPAYPEFLPWCGGTEILERQG
                     DITRAAIVIDFRGIKQRFSTQNRAEPPKLIEMTLIDGPFRQLDGSWRFKALGDEACKI
                     EFRLHYEFSSKLLEKIFGPVFHFIASTFVDAFVKRARQLYGER"
     gene            6147..6596
                     /gene="smpB"
                     /locus_tag="VN496_04170"
                     /pseudo
     CDS             6147..6596
                     /gene="smpB"
                     /locus_tag="VN496_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
     gene            complement(6630..7832)
                     /locus_tag="VN496_04175"
     CDS             complement(6630..7832)
                     /locus_tag="VN496_04175"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXF16174.1"
                     /translation="MFVDRVLSGMRPTGSLHLGHYHGVLKNWVKLQHEYQCLFFVADW
                     HALTTHYDTPEIVGQNVWDMLVDWLAAGVDPAAATLFIQSQVPEHAELHLLLSMITPL
                     GWLERIPTYKDQQEKLTEKDLSTYGFLGYPLLQGADILIYRANRVPVGEDQVPHIEFT
                     REIARRFNHIYGRETGFEQKAEIAVKKLGVKRGKVYRELRMRYQQKGEHEALTAAKDL
                     LEEAANLSMGDRERLFGFLEGGGKMILSEPHALLTEASKMPGLDGQKMSKSYNNTITL
                     REDADSVTKKIRTMPTDPARVRRSDPGDPDKCPVWEFHKVYSNDATKEWVQKGCRSAG
                     IGCLECKQPVIDAVLKEQEPMRERAQLYVDDPTLVRNIIAEGCDRARKLALETMRDVR
                     NAMGLNYP"
     gene            complement(7854..8501)
                     /locus_tag="VN496_04180"
     CDS             complement(7854..8501)
                     /locus_tag="VN496_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXF16175.1"
                     /translation="MEQTIRLIALYAIPGIFAITLHEAAHGYAARHFGDMTAYQAGRI
                     TLNPMRHVDVMGTIIIPILILIASQGRYAFGWAKPVPVNFSRLHNPKRDMFWVAAAGP
                     ASNLAMALVWAFVIKAMHGVPPNYFTEPLVLMARGGIVINAVLMVLNLLPLLPLDGGR
                     IAVSLLPQALAYRFGKLEPYGMIILLALMFLGVLNFVMLPFVNAFIGALAYVFKI"
     gene            complement(8507..9136)
                     /locus_tag="VN496_04185"
     CDS             complement(8507..9136)
                     /locus_tag="VN496_04185"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXF16176.1"
                     /translation="MTQHFVVHLQNPQQRVIRQAVEIVRKGGLMVYPTDSCYALGCHI
                     GDKAAMERIRRIRQLDERHHFTLICRDLSEVGQYAMVDNIQYRVLKSTTPGSYTFILK
                     ATREVPKRLLNPRHKTIGVRLPDHSVVKALLQEMGEPILSTTLLLPGDALPLNDMDEI
                     RSRLQHDVEVMLDAGSCGVEMTTVVDLTSDVPVIIRRGKGSIAPFTVPA"
     gene            complement(9146..9979)
                     /locus_tag="VN496_04190"
     CDS             complement(9146..9979)
                     /locus_tag="VN496_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXF16177.1"
                     /translation="MSIDLHTHSTYSDGVFTPAQLVLRAVSRGVKLLALTDHDETDGL
                     AQAAEAARGLDIVLVPGVEISTTWNNHTVHIVGLDIDVQSPRLVEGLASIRRCRGERA
                     TRIAAQLDAAGIAGSLPGAARHAGDSAAIGRSHFARFLVEQKHVRNTTDAFRRYLGSG
                     RVGYVAPVWPALPQAIDWIRCAGGRSVLAHPDRYHLTRQVLHNLLEAFASSGGDAIEI
                     SADGRFKSSAQVRLARRFGFALSSGSDFHGPGTNVPDVGEATQIPVGERAVWQELRTG
                     K"
     gene            10064..>10285
                     /locus_tag="VN496_04195"
     CDS             10064..>10285
                     /locus_tag="VN496_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HXF16178.1"
                     /translation="MTTIRFHALARLWRIAPLLVGYCVSPQAFSAHAQCEAHSSSNRV
                     AVLELYTSEGCNSCPPADRWLSSLPARGFT"
BASE COUNT         2159 a         3047 c         2836 g         2243 t
ORIGIN      
        1 tttttggcca atggaacttg acacggatct aaaatcgttc tgactcagat acttttggac
       61 gcctcttcgc gcatacggtt aacagtatcc ggtgccgcac gaacgcgaaa aagtgttcct
      121 gccccttctg tacgttcgtc cattacctcg caggtcgcaa agatctcgcc ccttaactgt
      181 tgcgccgccc acggcaggaa taattctgcc tcgataaggt ccttctggaa gaaagcccga
      241 atgctggaat gtagcgtcgc tatgtctgcc tcgcggcgtg cgctcatcac catgcaatcc
      301 gggtaccgct ggcgcagcgc ggtttcctgt gaggcctgcg ccgccgcatc gccgacgcag
      361 tcgatcttgt tgaacacgcg gatacggggc gcgcgctgtg cgccgatttc gttcaaaaca
      421 tcatcggtta cggcaagctg gcgctcgaat cccggatcgc ttgcatcgat cacgtgcaga
      481 agcagcgacg cttcggcggc ttcctcgagc gtcgacttga acgatgccac gaggtcgtgc
      541 ggcaggttct tgatgaaacc gaccgtgtca ctgatcagta cgcgcggcac gctatcggga
      601 tgcaaggcgc gcaccgtggt gtccagcgtc gcgaagagct tgttggcaac cagcacctcg
      661 cttccggtaa gtgcgcgcat taaagtggat ttgcccgcgt tcgtgtaacc gacgagcacc
      721 actcgcgcga gcccctggtg ctcgtggcga cgcgcgcgct gcgttttgcg ctcagcatcc
      781 attgccttga tttcttcctg cagttcggca atgcgatcgc gtattttctg ccggtccagg
      841 gcgctgtgag cttcccctgc cccacgtccg ccggtgccgc tgcgctgtcg gcccttcggc
      901 ccagccagct ttgcctgttc gcgcaaccgc ggtgccatgt atccgagccg cgcaatttcg
      961 acttgcgccc gcgctgctcg cgagcgcgca tgccggtgga agatttcgag gatgaccatt
     1021 gtgcggtcca tgacttcgca acctacctcg atctcgaggt ttcgcgcttg ggacggcgat
     1081 atctcgtggt cgaccagcac gatgtctgcg caacgggttt ctggatcggc ttcggatttc
     1141 gctgcttcct tggcacgggc cgccccgctt tttccccccg gccgcgcgtt gccggatgcc
     1201 acggactcat gcgttctctg ttccgtttcg ggctcgtttt ccacgaagcg gcgcatttcc
     1261 tgccgcttgc cgacacccat gtaggcagtc gtatcgaaat ggtcgcgtct ttgggtgaaa
     1321 gtgcccacga gttcgaatcc cagggtcttg gccagctcgc gcagttcgtt caccgaggag
     1381 ttgaactcga tatcgctcac tccggtgagc tgcactgctg caaccacggc ccgcaaggac
     1441 ttctcgcggc cctctggtcc catcgggcga ttactttctt caagggtcga acggtatttt
     1501 gccggagcga ccggacgcct cactcccatt cgatggtcgc cggtggtttt gaagcaatgt
     1561 catagaccac gcgattgatg ccccggactt cgttgatgat tcggttcgat accctggaaa
     1621 gcagcgtgta gggcagttcc gcccaatgcg cggtcatgaa atcgtcggtc tgcacagccc
     1681 tgagcgcaac aacgtattcg tacgtgcgcc cgtcgcccat cacaccgacc gaccttacgg
     1741 gcagaaatac cgcgaatgcc tgtgccgtct tctcgtacca gccggaagca cgcaattctt
     1801 cgatgaatat tgcgtcagcc cggcgtaaca attcggtgaa ctcactcttg atctccccaa
     1861 gaatccgcac accaagcccc gggcccggga acggatggcg aaacaccatg tcgtggggca
     1921 atcccagcgc caatccaagt tcgcgtactt cgtctttgaa taactcgcgc agaggctcca
     1981 gcagctttag atgcaaggtc tccgggagtc caccgacgtt atggtgcgac ttgatagtgt
     2041 gcgccttctt ggtctttgcg ccggcggact caatgacatc cggatagatc gtgccttggg
     2101 ccagccaccg ggcattgggc agtcgagccg cttcgcgttg aaatacttcc acgaattcgc
     2161 gtccgatgat cttgcgtttc tgttccggat cgctgatgcc tttgagcgcc gacatgaatt
     2221 gctcagaggc atccacggcg attactctga cgccgaggtt gccggcaaaa gtttccatga
     2281 cctggacagc ctcgttcagc cgcaggagcc cagtatcgac gaaaacgcag gtcagctgcg
     2341 ttccgatggc ccgatgaatt aaagcagccg ccaccgatga atctaccccg cccgacaaac
     2401 ccaggatgac ttcgtcattg ccgacctccg agcggatgcg cccaactgcc tcttcgaggt
     2461 aatcgggcat gttccactcc gatcgcaggc cgcagacacg gtgcagaaat tgctggatca
     2521 gcgccttgcc ctgcaacgta tgggtcactt ccggatggaa ctgcacgccg taaaacctgc
     2581 gggattcatc agccatgccg gcaatcggtg ttgcagcgtt gctcgcaatg atttggaaac
     2641 caggcggtag cgcgatcacc ttatcgccgt ggctcatcca gacatcgagc aagccatgcc
     2701 cctcggcgtt gcggcgatcc tcgagaccgt ccaggagtct ggaatgtccg cgcgcacgga
     2761 cttcggcata gccgaattcg cgtatcaaac cgttttcgac cacaccgccc agctgcgccg
     2821 ccattgtctg catgccatag cagatgccca acaccggtac ccccagatcg aacacggttt
     2881 gcggggcgcg gggtgtgcca tccacgacga ccgacgcagg gccacccgat agaataattc
     2941 ccttcggcgc gaaaccccgc acgaattcgt cgctaacgtc ataggggtgc aactcgcagt
     3001 acacccctgc ttcgcgcacg cgacgcgcga tcagctggct gtactgagcg ccaaaatcaa
     3061 ggataagaat tttatcgtgg gtcattcagt acagacgtgg tagccgacgc gagaaacggg
     3121 aagcccgcca gccggaaaat cccgtgcgcc ttgccgcgca tgcatgctta ttcgacgtga
     3181 tagttgggcg cttccttgac gatctgcaca tcatgtacgt gcgattcccg cactccagcg
     3241 gtcgtaattt gcacgaactc tgcgcgttgc tttacggttt cgatggtcgg acaacccagg
     3301 tagcccattg ctgaacgcaa gccgcccatc agctgatgca tgacctggac tacactacct
     3361 ttgtacggga cccggccttc cacgccttct ggcacgagct tgtccgcggc atcttcatcg
     3421 tcttgaaaat aacggtcgct cgatccctgc tgcattgcgc ccagagatcc catgccgcga
     3481 taagacttgt acgagcgtcc ctggaatagc tctatttcac ctggcgcttc ttccgtgccg
     3541 gcaaacaggc cgccaagcat taccgagtct gcccctgcag agatcgcctt ggcgatgtcg
     3601 cccgaatagc gaatgccgcc gtctgcaatc attggtacac cggtaccctt cagcgcggcg
     3661 gccacttcac tgatcgccgt gatctgcggc acccctacac ctgccacgat gcgggtcgtg
     3721 cagattgacc ccggaccaat accaaccttg acgccatctg caccctggtc gagcaatgat
     3781 ttcgccgctg ctgctgtagc aatgttgcca ccaatgacct gggcggccgg ataggtgcgc
     3841 ttgatccagc gcacccggtc cagcaccccc tgagaatgtc catgggccgt atccacaatt
     3901 agcacatcga cgccggcttc gaccagaaag cctacccgct cttctgtgcc ttctccaaca
     3961 ccgaccgctg cgcctacgcg caaacggccg agttgatcct tgctggcgta cggatgctcg
     4021 gaggacttca ggatgtcctt cacggtaatc aatccacgca attcgaattt ctcgttgatg
     4081 accagtacgc gttccagcct gtgctgatgc agcagacgca ttgcgtcttc gatactgacg
     4141 ccttcgcgca cggtcacaag tctgtcgcgc ggggtcatga tgtttttcac gggctggtcg
     4201 agattgcttt cgaatcgcag atcgcggttg gtgactatcc caaccacttt cgttccatcg
     4261 accaccggca gtccggaaat gcgatgctgc cggatgagat tgagtacgtt tcgtacggtc
     4321 atgtcctgcg agattgtgat ggggtccttc accacaccac tttcaaaacg ctttaccttg
     4381 gcgacttcgg cggcttgcgc tttaggtagc atgttcttgt gaacgatgcc gatgcctcct
     4441 tcctgtgcca tggcgatggc aaggcgcgcc tcggttaccg tgtccatggc tgccgaaacc
     4501 agcggtatat tgagcgatat gctgctggtt agctgagtcg tcaggctgac gtcacgagga
     4561 agaacggtcg aatgggcggg aaccagcaga acgtcatcga aggtgagcgc cttttgcagg
     4621 actcgcatgc ttttgatcct tcacaaagac gccattatac agaccgagtt tcgcgtggta
     4681 aatccaaggc ctgcggacgt tttacgttga ccgtcgcggt ccgagcagct atggttgttt
     4741 tatggcgctc catcggtaaa caacctgccg tttcaaccgt tgaatgcttg tgcgatcagc
     4801 ctcgaggaaa cgaccgcacg acacagttcc atagctcatc aggagaattt cgcatatgtc
     4861 atgcccattg ggtaacaccg cactgctgct tgccattgtc gtagcggcaa catcaggcgc
     4921 tgcgatcggc gcgcaaacct acaagtggac ggacgccgaa ggcaaggtcc attattccga
     4981 ccagccgcca ccggccaata tcaaggaacc aaccaccgta aagacgccgc ggcagtcagc
     5041 gccggcagcg ccgagtgctc caaatgcaaa gggtgcaccg cctcccaaag ccaagacata
     5101 caccgagcag gaagcggact tcaagaagag acaggtggag acggctgaac gcgaagctgc
     5161 ggaaaagaag aaagctgacg aggccgccga gaggaagcag acttgcacgc aggtgcgggc
     5221 gcaattgaaa ggtcttcagg ctggtggacg gctcactcag actaacgctc agggagagcg
     5281 cgaatacatg agcgatgcgc aaattggtca ggaaattgac cgtctgaaga aagcagaagc
     5341 gggcgtgtgc aactagcagt ccggccgttc agcggcgcgg ttttctctgt tgtcttgcgc
     5401 gctgccgtct tgcatgcttt ggatcggcaa ccagttcacg atagatttca attcggtcgc
     5461 gatcgcgtaa ttcggtttca ggcgacacga gtgctcccca gatgccaacg taattgcgcg
     5521 ccgtttgccg gcctgacaga ccggtagccg cgagcgcatc cgctacgcgc gccccttgct
     5581 cgagttccag aacgataact tcctgtgtgt gcggttgagc gtagaccact tccacccgga
     5641 tcttcatcgc tccccgtata actgtcgggc acgcttgacg aaggcgtcga cgaacgtgct
     5701 tgcgatgaaa tgaaataccg gcccgaaaat tttctccagc aacttgctgg agaactcata
     5761 gtgcaggcga aattcgattt tgcacgcttc atcgccgagt gccttgaacc gccaacttcc
     5821 atcgagctgg cgaaaaggac cgtcgatgag tgtcatctcg ataagcttcg ggggctcggc
     5881 ccggttttgc gtcgagaacc gctgcttgat gccacgaaag tcaatgacaa tggcggcgcg
     5941 ggtgatgtca ccctgtcttt ccagtatttc ggttccgccg caccagggta aaaactcggg
     6001 ataggcaggt atgttctcca ccagcgcgta catttgcgcg gcggaatatt cgatcaacac
     6061 ggatttctcc accagcgcca tcagatacgt ccgagccttc gtggaagcgt tggtaaaata
     6121 cacgacccgc agcaaaaact cacgccgtca tgagcatcgt tcagaataaa aaagcctttc
     6181 acgattactt tatcgaagaa cgctttgagg ccggcatgat tctggaaggc tgggaagtaa
     6241 agtcgatacg cgccgggcac gtgcagctca aggaagctta tgtggtcgtc aagagccagg
     6301 aaatctatct aatcggctgt catatcactc cgctgacggc agcttccacg cacatccaag
     6361 ccgaccctac gcgcacccgg aaactgctgc ttcacgtcga agagatccgc aagctgatcg
     6421 gcaaagtaga gcgagccggt tacacattgg ttccgctcga tcttcactac acgcgcggac
     6481 gggtcaaaat tgaggtcgga cttgcaaagg gcaaaaagca gcatgataaa agagaggccg
     6541 caaaagaaaa ggactggaaa cgcgaacagc aaaggctgat gcggcacaga acctgagtag
     6601 aaaagaaacg gcgccaggac ttcgcatcct cacgggtaat ttaaccccat ggcgtttcgg
     6661 acatcacgca tggtctccag cgccagcttg cgtgcgcggt cgcaaccttc tgcgatgata
     6721 ttgcgcacca aggttggatc gtccacatac agctgcgccc gctcgcgcat tggttcttgt
     6781 tctttcagca ctgcatcaat cacgggttgc ttgcattcaa ggcaaccgat gccagcgctt
     6841 cggcagccct tttggaccca ctccttcgtg gcgtcattgg aatagacttt gtgaaattcc
     6901 cacaccggac atttatccgg atcaccgggg tctgaacgtc gcacgcgcgc cgggtcggtt
     6961 ggcatcgtgc gtattttttt tgtgacgctg tcggcatcct cgcgtagcgt aatggtgttg
     7021 ttgtaagact ttgacatttt ctgcccgtcc agcccgggca tcttggacgc ttcggtcagt
     7081 aacgcatgtg gttccgacag aatcatcttg ccaccacctt cgaggaatcc gaacagcctt
     7141 tcccggtcac ccatcgatag attggcagct tcctcgagca ggtccttcgc cgccgttaac
     7201 gcctcgtgct cccccttctg ctgatagcgc attcgcagtt cacggtatac cttgccgcgc
     7261 ttcactccga gcttcttgac tgcaatctca gccttttgct cgaatcccgt ttccctgccg
     7321 taaatatgat tgaagcgacg cgcgatctcg cgcgtaaatt caatgtgcgg gacctgatct
     7381 tccccaaccg ggacgcggtt ggcgcgatag atcaggatgt ccgcaccttg cagcagcggg
     7441 taccccagga aaccataggt agaaagatct ttttccgtca acttctcctg ctggtccttg
     7501 taggtcggta tgcgttcgag ccacccgagc ggcgtgatca ttgacagcaa caaatgcagt
     7561 tccgcgtgct ccgggacctg agactgtatg aatagcgttg ctgctgccgg atcgacacct
     7621 gcggcgagcc agtcgaccag catatcccag acattctgtc cgacgatttc aggagtgtcg
     7681 tagtgcgttg tcagcgcatg ccaatcggcc acgaagaaaa ggcattggta ttcgtgctgc
     7741 agctttaccc agtttttgag cacaccgtgg tagtgcccga gatgcaaact gccagtcgga
     7801 cgcatcccgg ataacactcg atctacaaac attgattttt cctggttgct acttcagatt
     7861 ttgaagacgt aagcgagcgc cccgataaat gcgtttacaa atggcaacat gacgaaattg
     7921 agaacgccca aaaacatcag cgcaagcaga atgatcatgc cgtaaggttc aagcttgccg
     7981 aaacggtacg caagtgcctg gggaagaagg ctcaccgcaa tcctgcctcc atcaagggga
     8041 agcaaaggta ataggtttag aaccatcagc accgcgttga tgacaatgcc gccacgtgcc
     8101 atgagcacaa gcggttcggt gaagtaattc ggcggtacac catgcatcgc cttgatgacg
     8161 aaggcccaga caagggccat cgcaagattg gatgccggtc cggctgcggc aacccagaac
     8221 atgtcgcgtt tggggttgtg cagacggctg aaatttacag gaaccggttt ggcccagcca
     8281 aatgcatatc tgccctggct cgcaatcagg atcaagatcg ggatgattat tgtgcccatc
     8341 acatcgacat ggcgcatcgg atttaacgtg atgcgacccg cctgatatgc ggtcatgtcg
     8401 ccgaaatggc gtgcggcata accgtgagct gcctcgtgca atgttatggc gaatattccc
     8461 gggatcgcgt acagcgcaat caggcgaatc gtctgctcca gttcgatcac gcgggcaccg
     8521 taaacggcgc gatactgccc ttgccgcgcc tgataatgac agggacatca ctggtcagat
     8581 ccacgacggt ggtcatttcg actccacatg aaccggcgtc caacattact tcaacatcgt
     8641 gttgcagccg gcttcggatt tcatccatgt cgttgagagg caatgcatcg ccaggtagca
     8701 gcaaggtcgt gctcaaaatc ggttcaccca tttcttgcag cagcgccttc accacgctat
     8761 ggtcgggcaa tcgcacgcct atggttttgt gtctcggatt caaaagccgt tttggcactt
     8821 cccgggtggc cttcaaaatg aatgtataac tgccgggcgt ggtggatttg agcactcggt
     8881 attgtatgtt atcgaccatc gcgtattgcc cgacttcgga taagtcccgg cagatcagcg
     8941 tgaagtgatg ccgctcgtcg agctgccgaa ttcgacgtat gcgctccatg gctgccttgt
     9001 cgccgatatg gcatcccaac gcatagcagg aatccgtggg ataaaccatc aacccgcctt
     9061 tgcgcacgat ctcgaccgcc tggcggatga ctcgttgctg gggattttgc agatgtacaa
     9121 cgaaatgctg cgtcattccc ggaactcatt tgcctgtccg cagctcctgc cagactgcgc
     9181 gttcgccgac gggtatttgc gttgcttcgc ccacatcggg cacgtttgtt ccggggccat
     9241 gaaaatctga cccggatgaa agcgcgaatc caaagcgcct tgccagccgt acctgtgccg
     9301 aggacttgaa gcggccgtcg gcgctgattt cgatcgcatc gccgccgctg cttgcaaaag
     9361 cctccaacaa gttatgcagg acctggcgtg tcaggtggta acggtctgga tgggccagta
     9421 ccgatcggcc cccggcgcat cgaatccagt caatggcttg gggcagagcg ggccagaccg
     9481 gagctacgta gcctacccgg ccactgccga gatagcggcg aaaggcatcg gttgtattgc
     9541 ggacgtgctt ttgctccaca aggaaacggg cgaaatggct gcgaccaatg gcggcggaat
     9601 caccagcgtg tcgcgccgcg ccgggcaagc tgcccgctat gcccgcggca tcgagttgcg
     9661 cggcaatgcg tgtcgcacgt tcgccccggc agcgacggat tgacgctaat ccttccacca
     9721 gacgcggcga ctggacatcg atatccagtc ccacgatgtg gacggtatgg ttattccatg
     9781 tggtggagat ttcgacgccg gggaccagca caatatcgag gccgcgcgcg gcttcagcgg
     9841 cctgtgccag accgtccgtt tcgtcgtgat cagtcagggc gagcagcttg acacctctgg
     9901 ataccgcgcg gaggactagc tgggctggcg tgaacactcc atcggaatag gtggagtgag
     9961 tgtgcaaatc aatgctcaat gagcgttacg caaaggaatg agcatgcatc atagcaaaac
    10021 cgcttctatt gaaaaacctt taccgccgag aaggtcttcc tgcatgacaa ccatccgttt
    10081 tcacgctctg gcccggcttt ggcgcattgc tcccctgttg gtgggttatt gtgtctcacc
    10141 gcaagccttt tcggcacacg cacaatgcga ggcgcacagt tcgagtaatc gggtagcagt
    10201 gctggaactc tatacctccg aaggttgcaa cagctgtcct cccgccgatc gctggttgag
    10261 ttccttgccg gctcggggat tcacc
//
LOCUS       DATMZQ010000133         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1833001, whole genome shotgun sequence.
ACCESSION   DATMZQ010000133 DATMZQ010000000
VERSION     DATMZQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833001"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VN496_04200"
     CDS             complement(<1..790)
                     /locus_tag="VN496_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXF16179.1"
                     /translation="MEVTRRSFLTAAAATGIAVTSDAFAVNKHATDRDWTGEVTVTYP
                     DPAWEVMDKRFTARQGNATLQRIWHGMGPAASLWHEGPCWMGDWGCFLWSDIPNHRVL
                     RWTEDDGHVSVFQTESGYSNGHTRDREGRLIACEHDTRRVRRREHDGTWTVLMDSFNG
                     KKLNAPNDAVVASDGAIWFSDPGYGILGPYEGHKAEFELPTNVYRLDPQTGKATVVLG
                     SMRRPNGLCFSPDEKKLYIVDTAATDGPQYPANVMVFDVVSNGTS"
     gene            complement(1204..1827)
                     /locus_tag="VN496_04205"
     CDS             complement(1204..1827)
                     /locus_tag="VN496_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HXF16180.1"
                     /translation="MPDLPLFATPLAMYELSDMDEINRDVTTRLVAESLAKPSVTRSN
                     AGGWHSQSDLALRPQSCFRTLVQQIVNRVRETVDKQARERGANMPALRMGAQAWAMVM
                     RNGDYTIPHDHADAHWSTVYYPDAGDADEKSYPDSGLLAFVDPRHGGRPMPGLDLVGT
                     TFTAKASTGRLYVFPGWLFHYVHAYRGQRPRVSVSCNVTFELAAPLR"
     gene            2521..2646
                     /locus_tag="VN496_04210"
     CDS             2521..2646
                     /locus_tag="VN496_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16181.1"
                     /translation="MSEDDSETISIQDAIRLKHHVNKSIKYMAVTTQQSFGSLSN"
     gene            complement(3008..>3934)
                     /locus_tag="VN496_04215"
     CDS             complement(3008..>3934)
                     /locus_tag="VN496_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXF16182.1"
                     /translation="KAAAIHGRELLDHGFTIDQVVHAYGDVCQAITDLAFELHVPFEI
                     NEFRSLNRCLDEAIADAVTAFSDERDNLNAEKQSKETNIRLGALAHELRNLIQTVTLA
                     YAAIKAGTVGLSGSTGAVMDRTLASIRDLIDRSVAEVRMDAGMPMHSQIFALADLILE
                     VKISSTMEAEIHKCVFLVSSVDPQLAVQADRHLLSAAISNLLQNAFKFTHPGTEVTLS
                     AYGAGNRILIDVADHCGGLPWSDAEKVFVPFSQGGTDKRGLGLGLSISRRSVEANNGH
                     LRVQDRPGVGCVFTIDMPRRSTYECLGEARKQ"
BASE COUNT          877 a         1100 c         1092 g          865 t
ORIGIN      
        1 agctcgtgcc gttgcttaca acgtcgaaca ccatgacatt ggcaggatac tgtggcccgt
       61 cggttgcggc ggtatccacg atgtagagtt ttttttcgtc cggcgagaag cataggccgt
      121 tagggcggcg catgctgcca agcaccacgg ttgccttgcc ggtctgagga tcgagtcgat
      181 acacgttggt cggtaattcg aattcagcct tgtgcccttc ataaggccca agaatgccgt
      241 agccaggatc gctgaaccaa atcgcgccgt cgctcgcgac gacagcatcg tttggtgcgt
      301 tgagtttctt gccattgaag ctgtccatca acaccgtcca cgtcccgtcg tgctcacggc
      361 ggcgcacccg gcgcgtatcg tgttcgcaag cgatcagccg tccttcacga tcccgggtgt
      421 gtccattcga atagccggac tctgtctgaa acacactcac gtgcccatca tcctcggtcc
      481 agcgcaggac acgatgattg gggatgtcgc tccagagaaa gcagccccaa tctcccatcc
      541 agcacgggcc ttcgtgccat agtgatgcgg cgggacccat gccgtgccaa atccgctgca
      601 gcgtcgcatt gccctggcga gcagtgaagc gtttgtccat aacttcccac gccggatcgg
      661 gatacgtgac tgttacttcg ccagtccaat cgcggtctgt ggcgtgcttg ttgacggcaa
      721 acgcatcgct ggttacagca atgcccgttg ctgcagccgc cgtcaggaaa gaacgtctgg
      781 tgacttccat gccgatctcc tcttttctat gtttgattta ttgcgcgcac gatgggtgtg
      841 gacccaacag ttccgaatcc gcaaatcgtt agggcgaccc tgccccgaca ggtaaggtgg
      901 cgcatttttg tttcgtgaaa atttcatccc cgctgacagt attgctgtcc tgtaacccta
      961 gacattggcg cgcggataaa gcaaacgata gtgatggagt ccgggagggc gagagatcgt
     1021 gtttggagtg cgtcatgcgt ccgattgaga catgggagga cttatcgcga cgaaatccca
     1081 tcaatgcgca tgatcagcac aaaactgctt caggggaggg cagcggtgcc tgccgtgaag
     1141 cacgaaatgc tcgcgtgcgc ctaaacgaaa atgaaagagc gcttataacg gctggtttag
     1201 ttgttagcgc aacggcgctg ccagttcaaa agtaacgttg caggaaaccg atacgcgcgg
     1261 cctttgtcca cggtaggcat ggacatagtg aaacagccaa ccgggaaaca catacagcct
     1321 tcccgtgctg gcctttgccg tgaatgtggt accaaccagg tccaggcccg gcatcggccg
     1381 tcccccgtgt cgcggatcga caaacgccag caatcctgaa tcagggtatg acttttcgtc
     1441 ggcatcccct gcgtccgggt aatacaccgt agaccaatgc gcgtcggcat gatcgtgcgg
     1501 aatggtgtag tcgccattgc gcatgaccat agcccatgct tgcgcgccca tgcgcagggc
     1561 cggcatgttt gcgccccgtt cccgtgcctg cttatcgacc gtctcccgga cacggttcac
     1621 gatctgctgc accaaagtcc gaaagcagct ttgcgggcgc agcgccagat cagactgtga
     1681 atgccaaccg ccggcgttgc tgcgggtaac ggaaggcttg gccaacgatt cggcgaccag
     1741 ccgcgttgtc acgtcgcggt taatctcatc catgtccgaa agttcgtaca tggcaagcgg
     1801 cgtggcgaac agtggaagat ctggcatggc gttatggcga attttcttgg cgataacgga
     1861 ctaaccagat tgcggttgac agcgtcatgg tggtgatcca tgacgttaca actcgtcacg
     1921 ttttctaatc ggtttggaag gattgcggcg gcgaaaagcg ttcgtgcatc cgagacatcg
     1981 catttcaatg actcagctcg cttcggaccg gacagcggca tatattgaat ttctcgcgaa
     2041 aagtgctttg accgcaaggc agagctaaca tgcttgtcca gcatcccgcc ccggaggcgc
     2101 actacgcatc ctcgtggtcg aggactaccc ggatacctgc gaaacgaccg caaacgtttt
     2161 ggagttaaaa gggcttgtag ccaataccgc aacgagcggc catgaggcca tccgaaaggt
     2221 aagcgctttc gctcccgatc tggtgctgct cggcctttcc atgcctgcat atggatggta
     2281 tcgaagtcgc ccaaaccata ctcggaacgc acctggccag gaagcctgat gaacccattt
     2341 gtcctgcggg tatgggcggg ccatgttcgc cgcaagtcgg tcgaaataat gcgccacgcc
     2401 atcgtgcaga ctttcgcgcc aggcaggcgc ttcgagagtg cctgcagatg ggtccgcgat
     2461 gcagcccggc tacctactac gttcattgcc caggattgct ctcaaagaag ggagggaatc
     2521 atgagcgagg acgactccga gactattagc attcaggacg cgattcgcct caagcaccac
     2581 gtaaacaagt ccatcaaata tatggcggtt acaacgcaac agagcttcgg aagcttgagc
     2641 aattaagtga gcaacaacgt atcacgttac gagaaaacct cgcccacata aaaaggtatg
     2701 gtggtttttc ctgacacggg tcatgttgat cgggttcctg tcggcaagta tctcgctctt
     2761 ctatttcgtt agttgtgttt cagcaaaacg agaggttcgg ggaacatttt gtcccggcgc
     2821 agtccaatgc ggttggggac aataacgatg atcgtcgtca ctgccggttc tattgcgaaa
     2881 atgaagcagt tggaagccga tctgagggca caaggttgca gccgagttgg gcccttgtct
     2941 aatgcgcagc cttgatcgtg atctgcaccc gacgcagtca agagcaaatt gatagaaagc
     3001 aaaccattca ttgcttacgc gcttccccca gacattcgta cgtcgaacgg cgaggcatat
     3061 caatggtaaa aacacagccc acgccaggcc ggtcctgaac gcgcagatgg ccgttgtttg
     3121 cctcgacgct gcgacgggag attgacaatc ccaagcccag tcctcttttg tccgtgccgc
     3181 cttgggagaa gggcacaaaa actttctcgg catcgctcca cggcagtcct ccgcaatgat
     3241 ctgccacgtc gatcagaatt cgattcccgg cgccgtaggc gctcagcgtg acttcggtgc
     3301 ccgggtgggt aaacttgaat gcgttctgta gcaaattgct aatggccgcg gagagtagat
     3361 gccggtcggc ttggacggcc aattgcgggt ccaccgagga gactaaaaac acgcacttat
     3421 gaatctccgc ttccatggta gacgaaattt tgacttcaag gatcaagtcc gccagagcaa
     3481 aaatctggct atgcatcggc atgcccgcgt ccattcgaac ctcggctacc gaacggtcga
     3541 tcaggtcgcg gattgaggcc agtgtacggt ccataactgc cccggtggaa cctgacaacc
     3601 cgaccgtacc cgctttgatt gccgcataag ccagcgtgac ggtctggatg agatttcgca
     3661 gttcatgggc aagggcgcct aatcgtatat tcgtttcctt gctttgcttc tccgcgttga
     3721 gattatcccg ctcatcggaa aacgccgtga cagcatcggc gatggcctcg tccagacagc
     3781 gattgagtga acggaactca tttatttcaa aaggcacatg tagctcaaat gccaggtcag
     3841 ttatggcctg gcagacatcc ccgtaggcgt gcaccacctg atcaatcgta aaaccatgat
     3901 ccagtagttc gcggccgtga atcgcagccg cctt
//
LOCUS       DATMZQ010000134        14535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2522940, whole genome shotgun sequence.
ACCESSION   DATMZQ010000134 DATMZQ010000000
VERSION     DATMZQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14535
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522940"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1192
                     /gene="ybiB"
                     /locus_tag="VN496_04220"
     CDS             167..1192
                     /gene="ybiB"
                     /locus_tag="VN496_04220"
                     /inference="COORDINATES: protein motif:HMM:NF006005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HXF16183.1"
                     /translation="MSIARFIKQIVGTAGADRDLSEPDAYDLFAALLDGGVPDMELGA
                     CLCALVMKTQVASELLGFYRAASERMNLLQVPDTTLRPIIFAAYGGARQEPNLLPLLV
                     LLLQRLRVPVLLHGTLEGAGRVASAYILRELGVMPGRSLTEVQKSLDEEFLAFVPTAL
                     LCPGMANLLALRSRLGITNPAHSLVTLLDPFAAGGLRWLSTDEPQQLERLEMFLLATG
                     FDALLMRSTDGEPFANPRRRPRIKLFHRGEGTVLFEEELLATRGARCQLSAIDAHSTA
                     AWVKQAMAGETPIPHPLVNQLACCLFACGYTEDMNQAKAIAAVEAGSVHYKPKDCPVD
                     AVRFRAT"
     gene            1248..2024
                     /locus_tag="VN496_04225"
     CDS             1248..2024
                     /locus_tag="VN496_04225"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HXF16184.1"
                     /translation="MNMAIHPEQAFSTTITQDESLQLRIGHASASVKGKNNEDFHGIV
                     TQREESEARDRGIVIAVADGVSGNGGGRLASETAVKSLLRDFYAAPRAWSITRVLDKL
                     LLSINDWLFSENTRHPEYEGMVTTLTVLVFKDRFYHLAHVGDTRIYRSRGNVFKQLTI
                     DHTWERTDMRHVLKRAVGLDSHLVVDYTDGELLPGDVFLMASDGIWEVLGERVVCEAL
                     RTGLSTQAIAEDLVERSIQNQVQYMGRNDATAIVIAVDEV"
     gene            2095..2676
                     /locus_tag="VN496_04230"
     CDS             2095..2676
                     /locus_tag="VN496_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HXF16185.1"
                     /translation="MEHQLPPLPYPLDALQPHISKETLEYHYGKHHQAYVTNLNNLIK
                     GTEFANASLEEIVRKSSGGIFNNAAQVWNHSFYWNCLSPRGGGEPKGQLLEAINRKFG
                     SFSAFKEAFSKSAIGNFGSGWTWLVNKGNGTVDIVNTSNAGTPLTGPDKPLLTCDVWE
                     HAYYVDYRNRRPDYVAAFWNLVHWNFTASNFAA"
     gene            2947..3426
                     /locus_tag="VN496_04235"
     CDS             2947..3426
                     /locus_tag="VN496_04235"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXF16186.1"
                     /translation="MDMLPGIYRWIHILAGIMWVGLLYYFNFVQVAALKAAQGDGTAA
                     GITKHVAPRALFYFRWAALATWLAGAALLGSNFVAAFTFHAGFQKIGVGAWLGTIMLI
                     NVWGLIWPNQKKILGLVQATDEQKTKARRTAFLASRTNTALSIPMLFFMVAQTHIPM"
     gene            complement(3475..3933)
                     /locus_tag="VN496_04240"
     CDS             complement(3475..3933)
                     /locus_tag="VN496_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXF16187.1"
                     /translation="MRRFAFYFSFVQVVFLLGAMGAPALADPLVTHILRIKNREVRVE
                     IAMTEQTRLRGLMFRDNLAENSGMLFGYPRAEVSAMWMKNTKIALSVAFIDTSGRILN
                     IAEMEPFSEEPHASAGAAAYALEMNRGWFRKNGVKAGDRVEGLNKLPPAQ"
     gene            4411..6162
                     /locus_tag="VN496_04245"
     CDS             4411..6162
                     /locus_tag="VN496_04245"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXF16188.1"
                     /translation="MVKQSLHFKRAAVSLAAVAAFGASGVVSAQGLDSAESKLFPNYV
                     TDDMLLNADKDSQNWLHYGRDYQTTRYSPLTQVNRDNVKKLTPVWNLSFGVLEGQDSQ
                     AVAVNGTIYVTTSFNKVIAVDATSGRVQWKYERELPGDVFPKLCCDVVNRGVAVYKNK
                     VYLATLDAHIVALDSGSGKVVWDKKIGDYTYAETFTIMPMALRNKIIFGTAGAEYGVR
                     GWIAAIDADTGAPVSKTYTIPGPGEPGNDTWAGESWKYGGGSAWITGAYDKETNSLYW
                     PVGNPGPDFDRHVRLGDNLFTNSNLMLDPDTGKIRSYFNYTPNDPYDYDGVNEMILVD
                     VGGKKLWLHGSRNGHLYGIDRVNTAKGKAGNEHKCVWVTALQRVNWTKPITPANNCKP
                     VYNYPEKDVVYDKVTTDIAPSLDGGKEWHPMAYSHRSKMVYVPVYDFAMDLQAKKMEW
                     KRGEWYLGAKIINFNAGAGHTKAYDAATGKLAWQKSQSYPATGGMLATGGGLVFYGDP
                     EGYFNAVNDETGEHLWSFQTGSGIHGNPTSFTGPNGKQYVAIVYGAGGGGIWPIFYSE
                     FLKKNQKGGGLMVFAIE"
     gene            6245..7177
                     /locus_tag="VN496_04250"
     CDS             6245..7177
                     /locus_tag="VN496_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXF16189.1"
                     /translation="MATANRSTSGQRTSLYYRYWEKGECQKKELNVRKSFVRYGITFL
                     LTLMCVMVRAQTDPDEISDTLERSKARGKLLACADPYAFPSAAQNTDPPGYDVEIMRD
                     IAKRGGMRLEMVWADTGTRGGTSRAFRNSILRKRCDVFVGMSDNGDDDMLMGQLAFTK
                     PYIGMGYVLVVQGKAADKKSLAELKEANIKVGVSMSTPIDDYLFSRNIPRSLYLDNRR
                     ILQAMANGEIDAGMVWGTAISVARQEYPQAKFHMVDGYVPEPDQRWNLNFIVRKQDQS
                     LMKFIDDAVSDMLANGKIKQVVESYGVPFYPPFP"
     gene            7205..7672
                     /locus_tag="VN496_04255"
     CDS             7205..7672
                     /locus_tag="VN496_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF16190.1"
                     /translation="MSGLRLNAVTKLTATACFAVTLCVALPAAAQYKANSEDMEVPTN
                     DMKVVEAGRYMFGRRCAFCHGSDGHGAKGPCLSCGVFKYTGNTNSSIYVTIATGVPKN
                     LGGTMGAFGTTMSQEEILSVITFLRWEEKRRIDAKEIPDPYKNKNDEPMVFPK"
     gene            complement(7773..8222)
                     /gene="dut"
                     /locus_tag="VN496_04260"
     CDS             complement(7773..8222)
                     /gene="dut"
                     /locus_tag="VN496_04260"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXF16191.1"
                     /translation="MHNLDIKILDKRLRAQLPAYATAGSAGLDLRACINEPIQLHPGQ
                     TELIPSGLAIHLDEPGLAAFVLPRSGLGHKQGIVLGNLVGLIDSDYQGQILVSLWNRG
                     LSSFTLNPMERIAQLVVVPILQVSLNIVEEFSHSMRGADGFGSTGKH"
     gene            complement(8232..9440)
                     /gene="coaBC"
                     /locus_tag="VN496_04265"
     CDS             complement(8232..9440)
                     /gene="coaBC"
                     /locus_tag="VN496_04265"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXF16192.1"
                     /translation="MIELEHKRILLGLSGGVAAYKSAELLRELQRLGADIQVVMTDSA
                     CKFITPLTLQSLSGKPVYTHMWDNPASRGMPHIDLSRDRELIVIAPATADFIAKLAHG
                     AADDLLSTLCLARDCALMVAPSMNRQMWESPATQRNVAQLRLDGVMVLGPDAGDQACG
                     ETGMGRMLEPADLIREITAWFQPKLLSGAHVLVTAGPTFEPIDPVRGLTNRSSGKMGY
                     AVARASIEAGARVTLVTGPTAIEPPAAAQVERVQTAREMLDAVNCYENSIDIFIAVAA
                     VADYYIANGTTEKIKKEGAVPTLKLALNPDIVEAVAKWPNPPFCVGFAAETEKLEENA
                     KAKRRRKNLPLLAANLVASAIDADDNELVLFDDEGMHRLARSSKIEQARMLIRHVAKL
                     YKSHKAAVST"
     gene            9514..10188
                     /gene="radC"
                     /locus_tag="VN496_04270"
     CDS             9514..10188
                     /gene="radC"
                     /locus_tag="VN496_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXF16193.1"
                     /translation="MAIIDWPVDDRPREKLLAKGPGSLSDAELLAIFLRTGTKGKSAV
                     DIARSLLVRFGSLAGLLAAGRDAFCTVPGLGSAKYAQLHAATELVRRALSAQMKSGIN
                     LSSPEAVRDFLRLSIQDRKVEIFVGLFLDAQNCVIAVEELFSGTLTQTSVFPREVVRT
                     ALHHNAAAVIFAHNHPSGVAEPSRADETLTQALKQALALIDVRVLDHFVVGRGSTMSF
                     AERGLL"
     gene            10289..10525
                     /gene="rpmB"
                     /locus_tag="VN496_04275"
     CDS             10289..10525
                     /gene="rpmB"
                     /locus_tag="VN496_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXF16194.1"
                     /translation="MARVCQVTGKKPVVGNRVSHANNKTKRRFMPNLQYRRFWVESEN
                     RWVNMRVSNAGLRTIDKKGIDLVLAELRARGDKF"
     gene            10546..10701
                     /gene="rpmG"
                     /locus_tag="VN496_04280"
     CDS             10546..10701
                     /gene="rpmG"
                     /locus_tag="VN496_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXF16195.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRTTPDKIEMKKFDPVARKHVI
                     YKETKLK"
     gene            complement(10768..11973)
                     /locus_tag="VN496_04285"
     CDS             complement(10768..11973)
                     /locus_tag="VN496_04285"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXF16196.1"
                     /translation="MNVNGLMDLPGWANGLVVLPWWGYILAALLLTHITIAAVTIYLH
                     RYSAHRALDLHPVVSHFFRAWLWLTTGMSTKAWTAIHRKHHAKCETEEDPHSPQILSL
                     KKVLLEGSELYRKEAVNQETLAKYGHGTPDDWVERNVYSRTDKGGVAIMLIADILMFG
                     PLGLTIWAVQMLWIPVTAAGVINGVGHYYGYRTFQVEDASTNIVPWGILIGGEELHNN
                     HHAYASSARLSNKWYEFDIGWFYIRVLEMLNLAQVKKLAPQVKLEAGKSICDLQTLQA
                     VITHRYDVLARFTRSVRTTCAEELRRMREHKAGGHSVDLQTMKRWLHLDAKMVPEQEK
                     AKLDEVLSTSRVLKTVYLMRQELAMVWQRSTANKEQLLSQLEDWCRRAEKSDIGPLRD
                     FSRTLRCYA"
     gene            complement(12096..13406)
                     /locus_tag="VN496_04290"
     CDS             complement(12096..13406)
                     /locus_tag="VN496_04290"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXF16197.1"
                     /translation="MKNSSEVGHLLAGLLDDMQRGVVLWQLLIIALALLVAWHISRYM
                     RGRIVYTSSSDSERTMRISFGGMNRELFPMTALIIVVTGRWMLLHYEQPVNLLNIALP
                     LVLAMVIIRAAVYLLRHTFAPGGVLRSWEMAFSWLVWLGVAMHIAGLLPTIGRFLEDT
                     GFSVGQQRISLAMVLTALLTVILTVLAALWIGRVLETRIMALQHVEINLRFALSKILR
                     SVLVVVAVLIALPIVGIDITVLSVFGGAVGVGIGFGLQKVAANYVSGFTILLDRTVSP
                     GDLVTVDHFYGEVTKLTSRCIIVRGPDGTEAIVPNETVVTSTVINHSYSNRRVLVRVP
                     IQISYSSNLDVALKLMLEVANNHARVLKDPAPTALLTAFGDNGMNLELLAWIEDPERG
                     KGNLSSELNLSLYNSFRANSIEIPFPRRDVHIVGGVTLLDRGQK"
     gene            complement(13435..14004)
                     /locus_tag="VN496_04295"
     CDS             complement(13435..14004)
                     /locus_tag="VN496_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HXF16198.1"
                     /translation="MAVSRLVLLVIGYALVAPVHGFEASNVVQAVGTIEYALTPGNDA
                     AGLIVRTIDAAKSQVLVQAFSFTHRDIADALIRAHRRGIDVQIIADREQTDSMESSAL
                     RSLVRAGLPVFTDAEHSAAHNKVVVIDQNAEQPTLITGSFNFTFAAQFRNAENVLVLR
                     DNRELTRAFYSNWRQHRQHAVALGHSLNK"
     gene            complement(14008..>14535)
                     /locus_tag="VN496_04300"
     CDS             complement(14008..>14535)
                     /locus_tag="VN496_04300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXF16199.1"
                     /translation="MQSQGRLYAFDVSEKRLKNLNPRLKRSGLSNVHPQLIASENDIK
                     VKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPQSIDELAVKQEKILSASASLLKP
                     GGRLVYATCSVLGQENQAVVEQFLADHSSFEKISAKHELAENHIALDSDEYLKLCPHL
                     HDTDAFFAAIMQRRA"
BASE COUNT         3430 a         3771 c         3966 g         3368 t
ORIGIN      
        1 agccgatgcg tgatagcacg ttaacgcacc actcgcttgc cgagtctctg tacatttctg
       61 ctagatagct gcttttattg cccaaagttg actggcacaa cggttgcatt gtatccgcct
      121 cgacctcttc atcttcatcg ctgcggcagg acgtcaccag gcaactatga gcattgcccg
      181 attcatcaag cagattgttg gcacggcagg agccgaccgc gatctctccg agcctgacgc
      241 ctacgacttg tttgccgcct tattggatgg cggcgtaccc gatatggaac tgggcgcttg
      301 cctgtgcgcg ctcgttatga agacacaagt cgcgtcggag cttcttggat tttatcgcgc
      361 agcttcggaa cgcatgaacc tgctgcaggt accggacact actcttcgac caatcatttt
      421 cgcggcgtat ggcggagcca ggcaagagcc caacttgctt cctttgttgg tgcttttgtt
      481 gcagcgtttg cgcgtgcccg tccttttgca tgggacgctt gagggcgcgg gccgggttgc
      541 gagcgcgtac atcctgcgcg aactcggagt gatgcccgga agatcgttga ctgaagtcca
      601 aaaatcgctt gatgaggaat tccttgcatt cgttccgacg gcgctgctct gtccgggcat
      661 ggcgaatttg ctcgctttac gaagccggct cggcatcacg aatcccgccc acagtctggt
      721 caccctgctc gatccttttg ctgccggggg actgcgatgg ttaagcactg acgagccaca
      781 gcagcttgaa aggctcgaaa tgttcctcct ggcaaccggc tttgacgctc tattgatgcg
      841 cagtaccgac ggtgaaccgt ttgccaatcc caggcggcgg ccacggatca agcttttcca
      901 tcgaggtgag gggaccgtcc tgttcgagga agaactgtta gcaacgcgcg gtgcgcgatg
      961 tcaactttcg gccatcgatg cgcattcgac ggcggcgtgg gtgaaacagg ccatggctgg
     1021 cgaaacgccg attccgcatc cgcttgtcaa tcagttggca tgctgcctat ttgcgtgtgg
     1081 ttatacggaa gatatgaatc aggcaaaggc gatcgcggct gttgaggcgg gaagcgtgca
     1141 ttacaagcca aaggattgtc ccgttgatgc agtccgcttc cgtgcgacct gatcgttacc
     1201 ttcaatctta gtggcggact agctcgatcg gcacccaacc gaatctcatg aatatggcta
     1261 tccatcccga acaagccttc tcgacgacca ttacgcagga tgaatcattg cagttgcgaa
     1321 ttgggcacgc cagcgcaagt gtaaaaggga agaataacga agatttccac gggatcgtca
     1381 ctcagcgcga ggagagtgaa gcacgggaca gaggaattgt catcgccgtt gcagatgggg
     1441 tgagcggcaa tggcggcgga cgactcgcct ccgaaactgc tgtaaagagc ctgttacgcg
     1501 atttttacgc agcaccgcga gcctggagca ttacccgggt tctagacaag ttgttgctct
     1561 ccatcaacga ttggctgttt tccgaaaaca cgcgacaccc tgagtatgaa ggcatggtaa
     1621 ccacacttac ggtgttggtg ttcaaggatc gcttttatca cctggcacat gtaggcgaca
     1681 cgcggattta ccgatctcgg ggtaatgtct tcaagcaatt aacaatagat cacacgtggg
     1741 agcgtactga catgcgtcac gtgctaaagc gggcagtcgg gttagacagt catctcgtcg
     1801 tggattacac cgatggcgaa ctcttaccgg gtgatgtgtt tttgatggcg agcgacggga
     1861 tctgggaagt tttaggtgag cgtgtggtct gcgaggccct gcggacaggc ctatccacac
     1921 aggctattgc cgaagatttg gtggaacgat cgatacaaaa tcaggtccag tatatgggtc
     1981 gcaacgatgc caccgcgatt gtcatcgccg ttgatgaagt ttgaaacccc ggcaatgccg
     2041 ccattgcagc gcctttagcg tgcatgccta aaatactgac gaggaaacca gaaagtggag
     2101 catcaacttc ccccgttgcc ttatcccctg gatgcgttgc agccgcatat ttccaaagag
     2161 acactggaat accattacgg caagcatcat caagcctatg tcaccaattt gaataatctg
     2221 attaagggaa cggaatttgc caatgcttcg ctcgaggaaa tcgtccgaaa atcttcgggg
     2281 ggcattttta acaatgccgc gcaggtctgg aaccatagtt tctactggaa ctgtctgtcg
     2341 ccgcggggtg gtggtgagcc aaagggacag ttattggagg caattaaccg gaagtttggt
     2401 tctttcagcg cgttcaagga ggcgttttcg aagagtgcta tcggtaattt cggttccggc
     2461 tggacgtggt tggtgaataa gggcaacgga acagtagaca ttgtaaatac atcgaatgca
     2521 ggtacgccat tgacaggacc ggacaagcca ctattgacct gcgatgtctg ggagcacgct
     2581 tactacgttg actatcgcaa ccggcggcca gactatgtcg ccgcgttctg gaatctggtt
     2641 cactggaatt ttacagcgag taacttcgca gcctgataga tactgattgc attccagtct
     2701 ctagatcttt gcagttcgtc cagcttccag accacgcgta cggtcttcaa gcaaaaatcc
     2761 ccactatgac agtcgactat tgcggcgcgc cgccggcttc tctacactac tcgcggccga
     2821 taaacatgtg gaggaggaag taaaaatgac gaacccgctt gattctttat ggggcactgt
     2881 aatagttggt ctggtgctga ccgtcgtcct gtatttcata gtccggacaa tgtcaggagg
     2941 atagccatgg acatgctccc cggaatctac cgttggattc atatattggc cggcatcatg
     3001 tgggtcggct tgctgtacta cttcaacttt gttcaagtgg ctgcgttgaa ggcagctcag
     3061 ggtgatggca cggccgccgg cataacgaag cacgtggctc ctcgtgcact tttctatttc
     3121 cggtgggcgg cattggcaac ctggctcgcg ggcgccgccc tgcttggttc caattttgtc
     3181 gcggccttta cgtttcacgc cggctttcag aaaatcgggg ttggtgcttg gctgggcaca
     3241 ataatgttga tcaacgtatg gggcttgatt tggccgaacc agaaaaaaat ccttgggctg
     3301 gtgcaggcga cggatgaaca gaaaaccaaa gcgcgcagga ctgcttttct ggcttctcgt
     3361 acgaacacgg ctttgtcaat accgatgctg tttttcatgg tggcgcagac tcatataccg
     3421 atgtagcgcc gtcgcaatcg gacaagcgcc cggaatgttc cgggcgtttt gtttttactg
     3481 cgcaggtggt agcttgttta atccttcgac ccggtcgccc gctttgaccc cgttttttct
     3541 aaaccaaccc cggttcatct cgagcgcgta ggctgctgca ccggcggaag catgcggctc
     3601 ttccgaaaac ggttccattt ctgcaatgtt aagaatgcgc ccgctggtat cgatgaaggc
     3661 aaccgaaagg gcaattttgg tgttcttcat ccacattgca gacacttccg cacgaggata
     3721 gccaaataac atcccgctgt tttcggcaag attgtctcgg aacatcaatc cgcgaagccg
     3781 tgtttgctcc gtcatggcga tttcaacccg aacctcccga ttttttatac gcaggatatg
     3841 ggtgacgagc ggatctgcaa gcgctggagc acccattgcg ccaagcagaa atacgacctg
     3901 cacaaacgaa aagtaaaagg caaagcggcg catcgatttc gaatggtgaa acgataaatt
     3961 atagattctt gaatcgggtt gccgcgacct tgcgcttttt tacctcgcgt aaaaacccgg
     4021 ggtggcaact gcttatgcgg catgtgcaca tagaagcgga gtgcaacgtt cgctgcaatt
     4081 gtactagttg ccggcgtggg cgtaccggtc tccccgattg cgcgtggcca aggcggtcga
     4141 atgtgcggtg ccggctgaag cgctgcacgc ggttcatgcg ttattgatgc gacgcaataa
     4201 tttctgcgcc gtagggcaag ccgagagccg ggcagaaatg tgttgacaac atagtgcgta
     4261 actcttattt tcgccctgca gtaaagcttg tctagatctg aaatttcgga ttccccagtc
     4321 ttgctttacg catcgaggca gtaatcaaaa ccaaatgcgc ggcgtcgtaa gccaaaagcg
     4381 ctgcgcaact cttcgggagg tttttcaaaa atggtgaagc aatcgttgca cttcaaacgt
     4441 gctgcggtat ccctcgctgc agttgcagcg ttcggtgctt ccggggttgt ctccgctcaa
     4501 ggtctggata gcgctgaatc caagctcttt ccaaactacg taacggacga catgcttctg
     4561 aatgccgaca aggattcgca gaattggctc cattacggtc gtgactatca aaccacgcgc
     4621 tacagtccct tgacgcaggt gaatcgggat aacgtcaaga aactgacccc agtctggaac
     4681 ctgtcgttcg gtgttctgga aggtcaagac agccaagcgg tcgcagtgaa cggcacgatt
     4741 tacgttacga cgtcgttcaa caaggtcatc gcggtcgatg ctacatccgg cagggtgcaa
     4801 tggaagtacg aacgcgaatt gccgggcgat gtgttcccga aactctgctg cgacgttgta
     4861 aatcgcggcg tagccgttta caagaacaag gtttacctgg ctaccctgga tgcgcacatc
     4921 gttgcactgg acagcggttc cgggaaggtc gtctgggata agaagatcgg cgattacacg
     4981 tacgcagaaa cattcacgat catgccgatg gcgctgcgca ataaaatcat tttcggtaca
     5041 gcaggtgccg aatatggggt gcgtggctgg attgcagcaa tcgatgccga tacgggcgct
     5101 ccggtctcga agacatacac tattcccggg cccggcgaac ctggtaacga cacttgggcc
     5161 ggcgagtcct ggaaatatgg cggcggttca gcctggatta ctggtgccta cgacaaggaa
     5221 acgaattcct tgtactggcc cgtgggaaat cccggcccgg actttgaccg tcatgtgcgc
     5281 cttggcgaca acctgtttac caacagtaac ctgatgctgg atccggatac cggcaagatc
     5341 agaagctatt tcaactacac gcctaatgat ccgtacgatt acgacggcgt caatgagatg
     5401 attctggttg acgtcggcgg caagaaactc tggctgcatg gctcgcgcaa cggccacttg
     5461 tatggaatcg atcgtgtcaa tacagccaag ggcaaggccg gcaacgagca caagtgcgtg
     5521 tgggttacag cactgcagcg cgtgaactgg accaagccga tcactcctgc caataactgc
     5581 aagccggtct acaactatcc tgaaaaggac gtggtgtacg acaaggtaac caccgacatt
     5641 gctccgtccc tggacggcgg taaagagtgg cacccaatgg cttacagcca tcgcagcaag
     5701 atggtttacg tgccggtgta cgattttgcc atggacctcc aggccaagaa gatggagtgg
     5761 aagcgtggcg agtggtactt gggcgccaag atcatcaact ttaatgcagg tgcaggccac
     5821 accaaggctt atgatgcggc tacaggcaag ctcgcctggc agaaatccca aagctatcct
     5881 gcaaccggtg gcatgcttgc gaccgggggt ggcttggtgt tctacggcga tccagaaggc
     5941 tacttcaatg ctgtgaacga cgaaaccggt gaacatctct ggtcattcca gaccggctcc
     6001 ggaatccacg gcaacccgac ttcctttacc gggccgaacg gcaagcagta tgttgcaatc
     6061 gtgtacggtg cgggcggcgg cggaatctgg cccattttct actcggagtt tctgaagaag
     6121 aaccaaaagg gcggtggcct gatggttttc gctattgagt aatagtcaac tcaaagagta
     6181 gtacgaagct acagcaaggc taaggggggc gcactgagag ccccccttat tttttgtcaa
     6241 gggaatggcg acggcgaacc gttcaacgag cggtcagcga acgagcctgt attatcgtta
     6301 ctgggagaaa ggggaatgtc aaaaaaagga gttgaacgtg cgtaagtcat ttgttcgtta
     6361 cggcataacc tttctgctta cattaatgtg cgttatggtc cgggcgcaaa cggacccgga
     6421 tgaaattagc gacacgctgg aacgttcgaa agcgcggggg aaactcctgg cttgcgccga
     6481 tccgtacgcg ttcccgtccg ccgcgcaaaa tacggatccg cctgggtacg atgtcgaaat
     6541 catgcgggac atcgccaaac ggggcggcat gcggcttgaa atggtgtggg cggacacggg
     6601 tactcggggc ggcacgtctc gtgcatttcg caattccata ctcagaaaac gttgtgacgt
     6661 ttttgtgggc atgtctgaca acggtgatga tgacatgctg atggggcagc ttgcattcac
     6721 caaaccctat atcggcatgg gctatgtttt ggttgttcaa ggcaaggcgg ctgataaaaa
     6781 atcgctggcg gagctgaagg aagcgaacat taaagtcggt gtatcaatgt cgacgccgat
     6841 cgatgactat ctattcagcc gcaacatacc gagatcgctc tacctggaca atcgccggat
     6901 ccttcaggcg atggcaaatg gtgaaatcga cgcgggaatg gtatggggaa cggcaatttc
     6961 agttgcccgg caagaatacc ctcaggcgaa gtttcatatg gtggatggct acgtcccgga
     7021 gcccgatcaa cgctggaacc tgaattttat tgtccgcaaa caggatcaat cattgatgaa
     7081 gttcatcgat gacgcggtgt cggatatgct tgcgaatgga aagatcaagc aggttgtgga
     7141 aagctatgga gtgccgtttt atccgccttt tccataggat taatttaagg gaggtccgta
     7201 acacatgtca ggcctgagat tgaatgcggt aacgaagctg acggcgacag cttgttttgc
     7261 cgttacgttg tgcgtcgcct taccggcagc cgcgcagtac aaggcgaata gcgaggacat
     7321 ggaagttcca accaacgaca tgaaagtggt cgaagctggc cgatacatgt ttggacggcg
     7381 ctgcgctttt tgtcatggta gtgatggcca cggtgcgaaa gggccgtgcc tgtcgtgcgg
     7441 cgtgtttaaa tatacgggca ataccaacag cagtatttac gtcacgattg ccacaggcgt
     7501 gcccaaaaac ctcggcggta cgatgggggc gttcggtacg accatgagtc aggaagagat
     7561 actcagtgtg attacgtttt tacgatggga agaaaaacgc cgcatcgatg caaaggaaat
     7621 cccggaccct tacaagaata agaatgacga accgatggtt tttccaaaat gaagccttgg
     7681 tgttagaaag gcgagcatcg tggtgctctt cctcagtgac aaaaataaga agaccggcca
     7741 tttgccggtc ttcttatttc atgaaaggag cttcagtgct tcccagtgct gccgaacccg
     7801 tctgcgccgc gcatgctgtg cgaaaactcc tccacaatat tcaggctcac ctgcaggatt
     7861 gggacaacga ccagctgggc gatgcgctcc attggattca gcgtaaaact gctcaagcca
     7921 cggttccaga gcgaaaccag aatttgtcct tgatagtcgg aatcgatcag gccaaccaga
     7981 ttgcccaata cgatgccctg cttgtggcca agtcctgagc ggggtagcac gaatgcggca
     8041 agtccaggtt catccaggtg gatcgccaaa ccggagggaa tcagctcggt ctggcctggg
     8101 tgcagttgga ttggttcgtt aatacacgct cgaaggtcaa gtccggccga tcccgctgtt
     8161 gcataggcgg gaagctgtgc tcgcagacgc ttatccagga tcttgatgtc gagattatgc
     8221 atggcgacaa ttcaggttga aacggcagct ttatgtgatt tgtacaattt cgctacatgc
     8281 cggataagca tgcgtgcctg ctcgattttg gatgaacgtg ccagccgatg catgccttcg
     8341 tcatcgaaga gaaccagctc gttgtcatct gcatcgattg cggaagccac cagattggca
     8401 gccagcaaag gcagattttt gcgcctgcgc ttggccttgg cattctcttc cagcttttca
     8461 gtctctgccg caaaacccac gcaaaatggc ggattcggcc attttgcaac ggcttccaca
     8521 atatccgggt ttaatgccag tttcagcgtc ggcacggctc cttctttttt aatcttttcc
     8581 gtagtgccgt tggctatgta gtaatccgct accgcagcta cggcaatgaa tatatcgatg
     8641 ctattctcgt aacaattgac tgcatccagc atctctcgag ccgtttgtac gcgctcaact
     8701 tgcgccgccg ccggtggctc aatggcagta gggccggtaa ctaacgttac gcgtgcgccc
     8761 gcttcaatag acgcgcgtgc tactgcatag cccatctttc ccgagctgcg atttgtcaag
     8821 ccgcgtaccg ggtcgatcgg ctcgaatgtc gggccggcag tgacgagcac atgagcgccc
     8881 gataataatt ttggttgaaa ccaggcggta atctcacgaa tcaggtcagc cggttccagc
     8941 atgcgaccca tcccggtttc accgcatgcc tggtcgcctg catcggggcc aagcaccatg
     9001 acgccatcaa gccgcagctg tgccacattg cgttgcgtag cagggctttc ccacatttgg
     9061 cggttcatgg atggtgcgac catcaaagcg caatccctag ccaaacacag cgtggacaat
     9121 aggtcgtccg cagctccgtg cgctagtttt gcgatgaaat cagcggtggc cggcgctatc
     9181 acaataagtt cacgatcgcg ggacagatca atgtgcggca tgccgcggga agccgggtta
     9241 tcccacatgt gtgtatatac gggcttgccg gatagcgact gcagggtaag cggcgtgatg
     9301 aacttgcacg cggagtcagt catgaccacc tggatgtccg cgcccaagcg ctgtaactcg
     9361 cggagcagct ccgctgactt atacgcggcg actcctccgg agagtccaag aaggattcgc
     9421 ttgtgctcta gttctatcat ggaaatgtct ggcaagaatg ctcaaatggg agtgtaaact
     9481 attgttgcag aatataaaaa aatgtgaggg cagatggcga ttatagattg gccggtagac
     9541 gaccggcccc gggaaaaatt gctagcgaaa ggacctggca gcttgtcgga cgctgaattg
     9601 ctcgcgatct ttttgcgcac aggtaccaaa ggcaaaagtg cagttgatat agcccgttcg
     9661 ctcctcgtca gatttgggtc tttggccgga ttgcttgccg ctggccgcga cgctttttgt
     9721 accgttcccg gactcggcag tgccaaatat gcccagttgc acgccgcaac ggaactagtg
     9781 cggcgtgcgt tgtccgcgca aatgaagtca ggcattaatt tgagctcgcc ggaagccgtg
     9841 cgagatttct tacgcttgtc catccaggat cgaaaagtgg aaatttttgt gggccttttt
     9901 ttggatgcac agaattgcgt cattgccgta gaagaactct ttagtggaac actgacccag
     9961 actagtgttt tcccgcgcga agtagtgaga acggcgcttc atcacaatgc cgccgcagtg
    10021 atctttgcac ataatcatcc gtctggagta gctgaaccga gccgggccga tgaaacgcta
    10081 acgcaggctc tcaaacaagc gcttgcgtta atcgatgttc gagtgttgga ccattttgta
    10141 gtgggccgcg gtagcaccat gtcgttcgcc gaacgtggat tgctgtagcg ggttgagtaa
    10201 gtcgtgctaa ctatggtata aagcgcatct tccaaatttg gcgcgccgct gttttgcggt
    10261 tcgcttggga tacagtccgg agaatattat ggcaagggta tgtcaagtaa caggcaagaa
    10321 accggtcgtg ggaaaccgcg tatctcatgc aaataacaag accaagcgcc gctttatgcc
    10381 taatttgcaa taccgtcggt tttgggttga aagtgaaaac cgctgggtca atatgcgagt
    10441 cagtaatgcc ggcttgcgga cgatagataa aaaaggcatt gacttggtgc tggcagaatt
    10501 gcgcgcccgc ggcgacaaat tctaactagc tagcgggaga acgccatgag agaaaaaatt
    10561 aaactggagt cgtcagccgg aacagggcat ttttacacga ctacaaaaaa caaacgtacg
    10621 acgccggaca agatagaaat gaaaaaattc gatccagttg cccgtaaaca cgttatctac
    10681 aaagagacca agcttaaata gcgctggcct gccgggcaat gaaaaagccc gccaaccggc
    10741 gggctttttc catatcgcct tagctaatca cgcgtagcaa cgcagcgtcc gggagaagtc
    10801 ccgcagagga ccgatatcgc tcttctctgc acgacggcac cagtcttcga gttgcgaaag
    10861 cagttgttct ttgttggccg tagatcgttg ccagaccatt gccagttcct ggcgcattag
    10921 atagacggtt ttcaataccc tgctggtgct caacacttcg tccagttttg ccttttcctg
    10981 ttcgggcacc attttggcgt cgaggtgtag ccagcgcttc atagtttgta aatccaccga
    11041 atggccgccg gccttgtgct cacgcattcg cctcaattcc tcggcacagg tagtccgtac
    11101 ggaacgcgta aaacgcgcga gaacatcata ccggtgagtg atgactgcct gaagtgtctg
    11161 cagatcgcat atggacttgc cggcctccag ttttacttgt ggcgcaagtt tcttgacctg
    11221 cgccaggttc aacatttcga gcacccgaat gtagaaccag ccaatatcaa attcatacca
    11281 tttgttggac aaccgcgccg acgacgcata ggcatggtga ttgttatgta attcttcgcc
    11341 accaatcaaa attccccaag gaacgatatt ggtgctcgca tcttctacct ggaaggtgcg
    11401 gtatccgtaa tagtggccta cgccgttgat cacacctgcc gccgtaaccg gaatccacaa
    11461 catctgtacg gcccaaattg tcaacccgag cggaccgaac atcaggatat cggcgataag
    11521 cattattgcg acgccgcctt tgtccgttcg cgaatagaca ttgcgttcta cccaatcatc
    11581 aggtgttccg tgaccgtatt tcgcgagcgt ttcctggttc accgcttctt tacgataaag
    11641 ctcgctgcct tccagcaata ccttcttcaa gctaaggatc tgggggctat gtggatcttc
    11701 ctcagtctcg cacttggcgt gatgcttgcg atgaatcgcg gtccaagcct tggtggacat
    11761 gcccgtggtg agccacagcc acgcacgaaa aaaatgtgac acaacaggat gcaaatccag
    11821 cgcgcggtgc gcggaatacc gatgcaagta aatggttacg gcggcaatgg tgatatgggt
    11881 caacagcagc gcggccaaaa tgtagcccca ccacggaagg acaacgagtc cgtttgccca
    11941 acctggcagg tccataagcc cgttcacatt catgcaggtt acctcgataa atcagaagga
    12001 tatacaattc aggattgtac agtacgtgta tgttcgtgca aggtaatttt tggtaacaaa
    12061 gttccaacta ttggccgttt ttagaccaca aaagctcatt tttgtccgcg atccagcaac
    12121 gttactccgc caacgatatg gacatcccgt cgggggaacg gaatctcgat gctgttcgcg
    12181 cggaagctgt tgtagagcga tagattcagt tctgaactca gatttccctt gccgcgttcc
    12241 ggatcttcaa tccaggccag cagttccaga ttcatcccgt tatccccgaa tgcagtcagg
    12301 agcgccgtcg gagcgggatc tttcaaaacc cgcgcgtgat tgttggcaac ttccagcatc
    12361 agtttcaatg cgacatcgag attgctggaa taactgattt gaatcggaac gcgtaccaga
    12421 actcggcgat tggagtacga atgatttatt accgttgacg tgacaaccgt ctcatttggc
    12481 acaatagctt ccgtgccgtc agggcccctg acgatgatgc agcgcgacgt aagtttggta
    12541 acttcgccat agaaatgatc gacagttacc aggtcgcccg gactaacggt ccgatccagc
    12601 aatatggtga agccgctgac ataattcgca gcaacctttt gcaaaccaaa tccgataccc
    12661 acaccgacag cgccgccgaa aaccgatagc actgtgatgt cgatccctac gatcggtaat
    12721 gctatcaaaa ccgcgaccac caccaatacg ctgcgcagaa ttttggacaa tgcgaagcgc
    12781 aaattgattt cgacgtgctg caaggccatg atgcgcgttt ccaacacacg gccgatccac
    12841 aatgctgcaa gtaccgtgag tattacagtg agaagcgcgg taagcaccat cgctagcgag
    12901 atacgctgct ggcccactga aaaaccggtg tcctcaagaa acctgccgat ggtcggtaac
    12961 aagcccgcga tatgcatcgc gaccccaagc cataccagcc aggagaacgc catttcccag
    13021 gagcgcagaa ctcccccggg tgcgaacgtg tggcgcagca gatagactgc ggcgcggatg
    13081 attaccatcg ccaagactaa cggaagcgca atgttcaaca gattgaccgg ctgttcatag
    13141 tggagcagca tccagcggcc agtgaccact atgatcagtg ctgtcatcgg aaacagttcg
    13201 cgattcattc ctccaaaact gattctcatt gttcgttcgg aatccgacga actcgtgtac
    13261 acgattctcc cgcgcatata tctagagata tgccacgcga caagcagcgc cagcgcgatt
    13321 atgagaagtt gccacaggac tacaccgcgc tgcatatcgt ccaacaagcc ggcaagcaaa
    13381 tgacctactt ccgaagaatt tttcatcttc tctcccgtgg cgataacatt actgtcactt
    13441 gttcaggctg tgacccaatg caactgcatg ttggcgatgt tgacgccagt tgctgtaaaa
    13501 cgcccgggtc agttcgcgat tgtcccgtaa taccagcacg ttctccgcat tgcgaaactg
    13561 ggcggcaaag gtaaagttga aactgcccgt aatcagcgtg ggttgttccg cattctgatc
    13621 tatcacgacg actttattgt gagcggcgga atgctcggca tcggtgaaaa caggcaggcc
    13681 tgctctaaca agagaccgca gcgctgacga ctccatcgaa tcggtttgtt cccgatcggc
    13741 gattatctga acatcgattc cgcgccggtg cgcgcggatc agcgcgtccg caatatcgcg
    13801 atgggtgaag ctaaacgctt gcaccagcac ctgtgacttg gcagcgtcga tcgttcgcac
    13861 gatcaggccc gcagcatcgt tccccggagt caatgcatat tcgatcgttc cgactgcctg
    13921 caccacattg gaagcttcga agccatgaac cggcgctacg agggcatacc cgatcaccaa
    13981 gagtaccaag cgcgatactg ccatggctca agcccgcctt tgcatgatcg ccgcaaagaa
    14041 tgcgtcggtg tcgtgcaagt ggggacacag cttcaggtat tcatcgctgt ccagggcaat
    14101 atggttttcg gcaagctcgt gtttcgcgct gatcttttcg aaactggagt gatcggcaag
    14161 aaattgttcg actaccgcct gattttcctg accaagcacg ctgcaggtgg catagacgag
    14221 ccggcctcca ggcttcagca aggaggccga agcagaaagt attttttctt gttttactgc
    14281 gagttcatcg atcgactgag gcgattggcg ccacttcaaa tccgggttgc gccgcagagt
    14341 acccagcccg ctgcagggtg catcgaccaa tactcgatca atcttgcctg caagccgctt
    14401 tactttgata tcgttttcgc tggcaatgag ttgcggatgc acatttgaca gaccggaccg
    14461 cttaagcctg ggattgagat tcttcagacg cttttccgat acgtcaaacg catacagccg
    14521 tccctgggac tgcat
//
LOCUS       DATMZQ010000135        12406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2126918, whole genome shotgun sequence.
ACCESSION   DATMZQ010000135 DATMZQ010000000
VERSION     DATMZQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126918"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VN496_04305"
     CDS             <1..406
                     /locus_tag="VN496_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXF16200.1"
                     /translation="ALKNIKVEVELGFDAKLAFAEAQRCLNCDVQTVFSPPLCIECDA
                     CVDICPMDCITFTANGEEQELRSRLNAPAETLSQSLYVSDVLKTGRIMAKDEDVCLHC
                     GLCAERCPTGAWDMRKFLLDVTHAGPACRNPK"
     gene            388..2229
                     /locus_tag="VN496_04310"
     CDS             388..2229
                     /locus_tag="VN496_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXF16201.1"
                     /translation="MSQPKINDFVVKFANINGSGSASANQLFAKSILRMGVPVSPRNI
                     FPSNIQGLPTWYEVRVSEAGYLGARGGVDLMVAMNPQTWDKDVSGIDPGGYLFYDSTK
                     ALPKSKFRDDVRVIGVPLTDICNEAYSDPRQRQLFRNIVYVGALAALLDIDVVEIEKL
                     IGEQFQGKDKLIAPNIKALHLGRDYAKAHFECPIGLSVRRASAVENRIFVDGNTAAAL
                     GCVYGGATVAAWYPITPSSSLAEAFARHCRTYRKDAAGNNRYAVIQAEDELASIGMVI
                     GASWNGARAFTCTSGPGISLMQEFIGLAYFAEIPAVIFDVQRGGPSTGMPTRTQQSDV
                     LACAYASHGDTKHVLLFPEDPAESFDFAASAFDIAERLQTPVFVLLDLDIGMNERLSR
                     PLAWDDARRYDRGKVMSFEELDAGKEFGRYLDIDGDGITYRTYPGVHPSRGSFFTRGT
                     TKDRYARYSEAGPDYLDNMQRLLRKFESAKDVVPEPLIRAALQPSRLGVIYYGSTSPA
                     MHEALDTLEQRGLHFDAMRVRAFPFSHQVEDFIAEHDHVFVVEQNRDAQLRMLIVNEC
                     EIDPAKLTPVLHFDGTPITAHFIVREIAEDAAALKVTPMRKVLPGAR"
     gene            2257..3312
                     /locus_tag="VN496_04315"
     CDS             2257..3312
                     /locus_tag="VN496_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXF16202.1"
                     /translation="MTYIAKPKLHHPDLPVNKLGFTRRDYEGAISTLCAGCGHDSISA
                     AIIQACFELDLPPHRIAKLSGIGCSSKTPTYFLSSSHGFNSVHGRMPSVLTGANLANR
                     ELIYLGVSGDGDSASIGLGQFAHSVRRGVNMVYIVENNGVYGLTKGQFSATSDRGSRS
                     KKGSINTDAAIDMVVLALELGATYVARSFSGDKAQLVPLIKGAILHRGAAFIDCISPC
                     VAFNNHAGSTKSFDYVREHNEAVNQLDVMLPREPITADYAAGEVEEVTQHDGSVLKLR
                     KLAAEYDIHDRIAAMNYLQQRKAAGEIVTGVLHVDPDPEDLHAHLGTVESAFNSLRES
                     ELCPGSALLDQFNAAHR"
     gene            3401..4165
                     /locus_tag="VN496_04320"
     CDS             3401..4165
                     /locus_tag="VN496_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXF16203.1"
                     /translation="MNAFTPAELAQFDRDGFLIARGLVARSSCEAMKSVARRHLSANI
                     EPLEYESQVRYPGAPASFDAPGGRTVRRLLQAYARDELYRHWATCPEIVGRLSGLLGP
                     ELRLSQAHHNCIMTKNPAFSSATGWHQDIRYWSFEETQLVSAWFALGPEHEENGCLWL
                     VPGSHLMELRPEQYDGDLFFRADVLPNSSVLEAKQPAELDEGDVLFFHSRLLHAAGRN
                     HTDLTKFAAVFTYHAAENRPLPGSRSGSLPEIDLTA"
     gene            complement(4171..5055)
                     /locus_tag="VN496_04325"
     CDS             complement(4171..5055)
                     /locus_tag="VN496_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXF16204.1"
                     /translation="MPELPDIVVYVENLETRIMRRTLQQVRLLNPFLLRTAMPPISAV
                     HGKKVMGVQRMGKRIVIEIEGQLFLVLHLMIAGRLRWFDSKAKLPGRIALAVFEFENG
                     TLVLTEAGTKRRASLHLVEGESSARALHAGGIDVLESDLETFAQRIRAQSHTLKRALT
                     DPHILSGIGNAYSDEILHRAQLSPVALTGKLGDEELKRLYDSARATLLDWTDRLRTSA
                     AGNFPEKVTAFREDMAVHGRFGKPCPACGMPVQRIVYAENETNYCARCQTGGKLLADR
                     ALSRLLKKSWPRSIDDLE"
     gene            5200..5868
                     /locus_tag="VN496_04330"
     CDS             5200..5868
                     /locus_tag="VN496_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXF16205.1"
                     /translation="MEWARRISAGDEAAFEALMRRYNRMLYRVARAILRHDAEAEDAL
                     QLAYLQAYRGMGSYRGESKLSTWLTRIVVNESLMRARKRRREGTVIPLDSTVDQSDVA
                     MIENMESPDERPENAAIRSQTRALIERRIDALPEGFRAVFVLRALEELSVEETAASLG
                     IPEATVRTRFFRARALLRESLARDVDVATTEAFGFDGARCDRIVTAVLLRVRRPDTAQ
                     HRVR"
     gene            5897..6352
                     /locus_tag="VN496_04335"
     CDS             5897..6352
                     /locus_tag="VN496_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXF16206.1"
                     /translation="MQSLTLFAGLAMPVMEWNEALVLDKGIMDETHRQFIELLNRLAD
                     AADEDILPVLDEFISHTQEHFAQEQEWMEQMAFPPLACHAREHEGVLETAQEVRRRAL
                     AGETRFGRVLAKAVAEWFASHAASMDHVLALYMKEKGYDPKPSSVTAKT"
     gene            complement(6373..6927)
                     /locus_tag="VN496_04340"
     CDS             complement(6373..6927)
                     /locus_tag="VN496_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXF16207.1"
                     /translation="MSLQTLKDRLPDYAKDLKLNLGSLAAETLLDDTLKAGTFVAAAF
                     GSRNPMVIEAINAEFAGKLSPADFTAAKAAAAIMGMNNVYYRFVHLSDSAEYGRLPAR
                     LRMNIIGTPGTAKENFELWSLAVSAINGCGMCITAHEKVVRAAGLSAEQVQAAVRIAA
                     VVHAVAVVLDAEGAAKAEPFAAAA"
     gene            complement(6999..7538)
                     /locus_tag="VN496_04345"
     CDS             complement(6999..7538)
                     /locus_tag="VN496_04345"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXF16208.1"
                     /translation="MLTVGERIPAFDLNAVVSIDPDKAFQRIDQATNPGKWKVLFFWP
                     KDFTFVCPTEIAGFGKLNKDFNDRDAVIYGVSIDSEFVHLAWRNNHPDLKNLPFPMLA
                     DIKRELSSALGVLHKTEGVALRATFIVDPEGIIRFVSVNDMNVGRNPEEVLRVLDALQ
                     TDELCPCNWKKGDEFLKAA"
     gene            7664..8605
                     /locus_tag="VN496_04350"
     CDS             7664..8605
                     /locus_tag="VN496_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HXF16209.1"
                     /translation="MAALPSLRQLGYLVEVADRLNFRAAAEARFVTQSTLSAGIKELE
                     SLLGVELVERDPRGVRLTPAGEEVTRRARGLLASANDLVESARGAGVPLGGVFRMGVI
                     PTIAPFLLTGMMTDLRAEYPDLKLHLREDITDRLLEKLRAGELDAALIALPYAISELI
                     AIKLFRDEFWFVAHEDDPHAKKKEILVRDLEPGSVVLLEEGHCLRDHAIAACGARGAK
                     AKGLLEATSLTTLIQMVEGGLGVTLLPELMIKAGVLKGTHLIARPFSTDIPARTVALV
                     ARPASARRAGADLLADFFQRHYRRQSKVRTASRSNKR"
     gene            complement(8976..11621)
                     /gene="pepN"
                     /locus_tag="VN496_04355"
     CDS             complement(8976..11621)
                     /gene="pepN"
                     /locus_tag="VN496_04355"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HXF16210.1"
                     /translation="MLTSTQKTIFLKDYTAPAFLVSTLDLDVDLQDDYAVVSAKLAIA
                     RNAAADNAMAALKLDGDELELLEVLLDGHSLGPRDFTLSNECLEIPNVPDRFTLTTRI
                     RIRPKQNTKLMGLYASRDGYFTQCEAEGFRRITFFPDRPDVMARYTTTVHADKAAFPF
                     LLSNGNQIAQGEEAGGRHWARWVDPFPKPSYLFALVAAKLDRLDDSFQTRSGRRVTLS
                     VFVEPGKLDQCSFAMQALKRAMKWDEEVFGLELDLDQYMIVAVGDFNMGAMENKGLNI
                     FNTKYVLARADTATDTDFMFLDRVVAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQ
                     EYGADTYSRPLQRIQEVRSLRSAQFPEDAGPMAHPVRPESYMEISNFYTATVYEKGAE
                     VVRMIHTLIGALSFRKGMDLYFERHDGQAVRTEEFVQAMQDASGVDLAQFKRWYEQAG
                     TPLLEIAARYDAIGCRYTLTVKQSCPPTPGQPHKKPLHIPIALGLVAPDGSEVPLQLA
                     GEAQSHGSTRVLSLTKPEEQFEFVNIAQRPVPSLLRGFSAPVNLRFDFSAAELTHLMA
                     HDADAFNRWEAGQRLALDLLLRALNGARAGRAAEFPGSFATAFARVLADAPKDPAFAA
                     EALALPSEGYIAEQVDEIDPDAIHLVRRSLRHHIAAALKTELLAFYRSSSAPRPYSPD
                     ARSAGLRALRNLCLGYLMELDDAEIRALCFTQFDTADNMTDAMAALTALANTEATERE
                     AALRLFYEKWQHEPLVIDKWFAVQATSRLPETLSVVKRLIQHPAFSLKNPNRVRSVIG
                     SFCHGNQVRFHAPDGAGYSFAADQIIAIDRLNPQVAARLARAFDRWKKFDAARQTRAQ
                     AALEHIRGAAGLSKDTIEVVTRALA"
     gene            11620..11748
                     /locus_tag="VN496_04360"
     CDS             11620..11748
                     /locus_tag="VN496_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16211.1"
                     /translation="MIAMGERLMIDHEFYRGVPSVDAQAKKGRREAPNRGGNYNNQ"
     gene            complement(11742..>12406)
                     /locus_tag="VN496_04365"
     CDS             complement(11742..>12406)
                     /locus_tag="VN496_04365"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXF16212.1"
                     /translation="GGWDNERMTMTMNINIAASFSSVLIVMAAPAFASNYAAADYMDS
                     APVVSSVPVYQTVRQPQQQCWTESVTSYEERRSPSGLILGGVAGGIAGNAIGRGNGRV
                     ASTVVGAIIGAAIGDHLANRDGNAVVVNRPVQHCQTVESYHQVLTGYQVTYDYNGRYS
                     TIMLPYDPGPRLPLEITVARPGPPAYLAPPVDRVMYEHRHVPAWEQPVYKRPRHYYSS
                     VH"
BASE COUNT         2599 a         3556 c         3632 g         2619 t
ORIGIN      
        1 cgcgctcaag aacatcaagg tggaagttga actgggcttt gatgcgaaac tggcgttcgc
       61 cgaagctcaa cgctgcctca actgcgatgt gcagacggta ttttcgccgc cgctttgcat
      121 agaatgcgat gcctgtgtgg acatctgtcc gatggattgc atcacgttca ccgccaacgg
      181 tgaagagcaa gaactgcgca gccggctgaa cgcaccggcc gagacgctgt cgcagagttt
      241 gtatgtatct gacgtgctga aaaccggtcg tatcatggcc aaggacgaag atgtctgtct
      301 gcattgcggg ttgtgtgccg agcgctgtcc gaccggcgct tgggacatgc gcaagtttct
      361 attggacgta acgcacgcgg gaccggcatg tcgcaaccca aaataaacga cttcgtagtc
      421 aagtttgcga atatcaacgg ttccggctca gcgtctgcca atcagctttt cgccaaatcg
      481 attctgcgta tgggcgtccc ggtcagcccg cgcaacatct ttccgtccaa catccaggga
      541 ctgcccacct ggtatgaagt gcgtgtctcg gaggccggct atctcggcgc acgcggcggc
      601 gtcgacctca tggtcgcgat gaatccgcag acttgggaca aggacgtgtc gggcatcgat
      661 ccgggcggtt atctgtttta cgattcaacc aaagcccttc cgaagtctaa atttcgcgat
      721 gacgtacgcg tcatcggtgt gccgttgacc gatatctgta acgaggcgta ttccgatccg
      781 cgccagcgac agctatttcg caacattgtc tatgtcggcg ccctcgctgc attgcttgac
      841 atcgacgtcg tggaaatcga aaagctcatc ggtgagcagt tccaggggaa agacaagctc
      901 atcgcgccga acatcaaggc gctgcacctg gggcgcgact atgccaaagc gcatttcgaa
      961 tgtccgatcg ggttaagcgt gcgccgtgca tccgcagtgg agaatcgcat ctttgtcgat
     1021 ggcaatactg cggccgcatt gggttgcgtc tacggcggag caaccgttgc agcctggtat
     1081 cccatcacac cgtcgtcgag ccttgctgaa gcgttcgcgc gccactgccg gacttaccga
     1141 aaagatgcgg ctggaaacaa tcgctacgcg gtgatccagg ccgaggacga gcttgcctcc
     1201 atcggcatgg tgatcggcgc cagctggaac ggagcacgag ctttcacctg cacatccggt
     1261 cccggtatct cgctgatgca ggaattcatt ggcctcgctt atttcgcgga gataccggcg
     1321 gtcatttttg acgtccagcg cggcggacct tccaccggca tgcctacacg cacgcagcag
     1381 tctgacgtcc ttgcctgcgc ctatgcgtcc cacggcgata ccaaacatgt gctgttgttt
     1441 ccggaggatc cggccgaaag tttcgatttc gcagcgagcg cgttcgatat tgccgaacgt
     1501 ctgcaaacgc cggtgttcgt actgctcgat ctggacattg gtatgaacga gaggttgagc
     1561 cggccgcttg cctgggacga cgcccgtcgc tacgatcgtg gcaaggtgat gagcttcgag
     1621 gaactcgacg cgggcaagga attcggccgg tatctggaca tcgatggcga cggcataacg
     1681 taccgcacct atcccggcgt gcatccttcg cgcggatcgt tcttcacgcg cggcacgacc
     1741 aaggaccgct atgcgcgcta cagcgaggcg ggtccggatt accttgacaa tatgcagcgg
     1801 ctgttgcgta aattcgagtc ggcaaaggat gtcgttcccg agccgctgat tcgtgccgct
     1861 ttgcagccta gccggctggg ggttatctac tacggttcca ccagtccggc catgcatgaa
     1921 gcgctcgata ccctcgagca gcgtggattg cacttcgatg cgatgcgtgt gcgcgcattc
     1981 ccgttcagcc accaggttga ggatttcatc gctgaacacg atcacgtatt cgtggttgag
     2041 cagaatcggg atgcgcaatt gcgcatgctg atcgtaaacg aatgcgagat cgatcccgca
     2101 aagcttacac ccgtgctgca cttcgatggc acaccgatca cggcacattt tatcgtcagg
     2161 gaaattgcgg aggacgccgc ggcactgaaa gttacaccga tgcgcaaggt actgcccggg
     2221 gccagatgag ggcgcgctgc taaattagga catgacatga cttatatcgc caagcccaag
     2281 cttcatcatc ccgaccttcc cgtaaataaa ttgggattta cacggcgcga ctacgaaggc
     2341 gccatttcca cgctttgcgc cggctgcggc catgactcga tcagtgcagc aatcatccag
     2401 gcgtgtttcg agcttgacct gccgccccac cgcattgcca aactttccgg cattggttgt
     2461 tcgtccaaaa ccccgacgta cttcctgagc agttcgcatg gattcaacag cgtgcatgga
     2521 cgcatgccgt ctgtcctcac cggcgccaat ctggcgaacc gagaactgat ttacctcggt
     2581 gtgtcggggg atggtgattc cgcctcgatc ggactgggcc aatttgcgca cagcgtccgc
     2641 cggggcgtga acatggttta cattgtcgaa aacaatggcg tctacgggct gaccaaaggc
     2701 caattttcgg caacgtcgga tcggggctcc aggagcaaaa aaggctcgat caataccgac
     2761 gctgccattg atatggtggt actggcactg gaattggggg cgacttatgt cgcacgcagc
     2821 ttttccggcg acaaggcaca actcgtgccg ctgatcaagg gggccatcct gcatcgcggc
     2881 gcagctttta tcgattgcat ttccccgtgc gtggcgttca acaaccacgc cggttcaacg
     2941 aaaagcttcg attacgttcg cgaacacaat gaggcggtca accagctcga tgtgatgctg
     3001 ccgcgggaac cgatcaccgc ggattatgct gccggagaag ttgaagaagt tacccaacat
     3061 gacgggtcgg tgctcaaatt gcgcaagctg gcggccgaat acgatattca cgatcgcatc
     3121 gcggcgatga attatctgca gcagcgcaag gcagccggag aaatcgtaac gggtgtcctg
     3181 cacgttgacc cggatccgga agacctccat gcccatctgg gcaccgttga gtccgcattt
     3241 aacagcttgc gcgaaagcga gctttgtcct ggaagcgcgc tcctggatca atttaatgcc
     3301 gcgcaccgct aacgcatagc ggcgaacaat cctctgctgt gatggtccct gctgcgtccg
     3361 ccgacacggg cgttgatatt cgcttttgcc ggctcggccc ttgaacgctt ttaccccggc
     3421 agaactcgcc cagttcgacc gcgatggttt cctgattgcg cgcggcctgg tggcgcggtc
     3481 ttcgtgcgaa gcgatgaaga gcgtggcacg gcgccacttg agcgccaaca tagagccgct
     3541 ggaatacgag tcccaggtcc gctatcccgg cgcgccggct tcgttcgacg caccgggcgg
     3601 gcgtaccgta cgtcgattgc tacaggcgta cgcgcgcgac gaactctacc gccattgggc
     3661 aacgtgcccg gaaatcgtcg ggcgcttgag cggcctgctc gggccggaac tacggctctc
     3721 acaggcccat cacaattgca tcatgaccaa gaacccggcc ttcagcagtg cgacaggatg
     3781 gcatcaggac attcgctatt ggtcattcga agaaacccag ctggtctcgg cttggtttgc
     3841 gctcgggccc gaacacgagg aaaacggctg cctctggctg gtgccgggtt cccacctgat
     3901 ggaattgcgc ccggaacaat acgacggcga cttgtttttc cgtgcagacg tgcttccgaa
     3961 cagcagcgtt ttggaagcaa aacaaccggc cgaactggac gagggcgacg tgctgttctt
     4021 tcacagccga ctgctgcatg cggctggacg caaccacacg gatctgacga agttcgcagc
     4081 cgtttttacc tatcacgctg cggagaaccg tccattgcca ggcagccgct cgggttcttt
     4141 gcccgaaatt gatttaacgg cctagggaag tcattccaga tcgtcgatgg agcgcggcca
     4201 gcttttcttc agcaggcgcg aaagggcgcg atcggccaga agcttgccgc cggtctggca
     4261 gcgggcacag tagttggtct cattctccgc gtacacaatc cgctgcaccg gcatgccgca
     4321 tgcgggacac ggtttgccaa agcgcccatg tactgccatg tcttcccgaa atgccgtgac
     4381 tttctccgga aagttgccgg ccgctgatgt gcgcaatcgg tcagtccaat cgagcagagt
     4441 ggcgcgggcc gagtcgtaca ggcgtttcag ttcttcatca ccgagctttc cggtcagggc
     4501 aacgggtgag agctgcgcgc ggtgaagaat ctcatcagag tatgcgttgc caatgccgct
     4561 gagaatgtgc gggtcagtca gcgcgcgctt gagcgtgtgg ctttgggctc ggatccgctg
     4621 cgcaaaggtt tcaagatctg actcgaggac atcgatccca ccggcgtgca gcgctcttgc
     4681 tgaagattcg ccttcaacca ggtgcagcga tgcgcgtcgc ttggtgccgg cttccgtcag
     4741 aaccagcgtc ccgttttcga attcgaatac agcgagcgca attcgtccgg gaagctttgc
     4801 cttgctatcg aaccaacgca gccgccccgc gatcatcaaa tgcagcacaa ggaatagctg
     4861 gccctcgatt tcgatgacta tgcgcttgcc catgcgttgg acgcccatga ctttcttgcc
     4921 gtggaccgcc gatatgggcg gcattgcagt gcgtagcaag aatgggttca gcagacgaac
     4981 ttgctgtaga gtccgtcgca taatgcgtgt ctcgagattc tccacgtaca cgactatgtc
     5041 aggtaattcg ggcatgggct tctgtgaatt tgcggctttc atatcccgga tcgggaacgc
     5101 tcgctgcccc gggtgcatca aatggtcaga ccaccggtgg agtgtatcgt gcccgtaagc
     5161 gcccaagtgc aaacccgcct gcccaacgac ctgagtgata tggagtgggc ccgacgaatc
     5221 tcagctggag acgaagcggc tttcgaggcg ctgatgcgta gatacaatcg gatgctgtac
     5281 cgggttgcac gcgccattct gcgccatgat gcggaagccg aggatgcctt gcagctggca
     5341 tatcttcagg cctatcgcgg catgggaagc taccgggggg aatcaaaatt gtccacctgg
     5401 ttaacccgca ttgtcgtgaa cgaatcactg atgcgcgcgc gcaagcgtcg ccgtgagggg
     5461 acggtaattc cactcgattc cacggtagac caatcggacg tggcaatgat agaaaacatg
     5521 gaaagtcctg atgagcgtcc ggaaaatgca gcgatccgca gccagacgcg cgccttgatt
     5581 gagcgccgca tcgacgcact tccagaagga tttcgcgcgg tgtttgtgct gcgcgcgctc
     5641 gaagagctgt ccgtggagga aactgcagcc agcctcggca taccagaggc aaccgtgcgc
     5701 acccgctttt tccgcgcccg cgccttattg cgggagagcc tggcacgcga tgtcgatgtc
     5761 gcaaccacag aggcttttgg attcgatggc gcgcgatgcg accgcatcgt aaccgccgta
     5821 ctccttcggg ttcgacgacc ggatacggcc caacaccgag tacggtagcg cgccggaata
     5881 gtgaacggcg ggtaaaatgc agtccctaac cttgtttgcg ggacttgcca tgccagttat
     5941 ggaatggaac gaagctctgg tgctcgacaa aggcatcatg gacgaaactc atcgccagtt
     6001 catcgaactg ctcaaccgtc tggcggacgc tgccgacgaa gatattttgc cggtcctgga
     6061 cgagtttatc tcgcacaccc aagagcattt cgcgcaggag caggaatgga tggaacagat
     6121 ggctttcccg ccgctggcct gtcacgcaag ggaacacgaa ggcgtacttg aaaccgcgca
     6181 ggaagtgcgt cgccgcgcgc tggcagggga gacgcgcttc ggccgcgttc tggcgaaggc
     6241 cgtagccgaa tggtttgcca gccacgcggc cagcatggac cacgtgctgg ccctgtacat
     6301 gaaagaaaaa ggctacgacc ccaagccttc gtccgttacc gcgaagacgt aacggacgga
     6361 cgagccttgg tgctatgccg ctgcggcgaa cggttcggct ttggccgcac cctcggcatc
     6421 caatacgacc gccaccgcat gcaccactgc agctatccgt acggcagcct gtacttgttc
     6481 ggcgcttaat ccagcagcgc gcaccacctt ttcgtgtgcc gtgatacaca ttccgcaacc
     6541 gtttattgcg gaaaccgcca gcgaccacaa ttcgaaattt tcctttgcgg tgcccggagt
     6601 cccgattatg ttcatgcgca atctggccgg taggcgcccg tactccgcgc tgtccgacaa
     6661 atgaacaaag cggtaataga cgttgttcat gcccatgatg gccgccgcgg ccttggccgc
     6721 agtgaaatca gccggactta acttgcctgc gaattccgca tttatcgcct cgatgaccat
     6781 cgggtttcgg gaaccgaaag cagcggcaac gaatgtgccg gccttcagcg tatcgtccag
     6841 aagcgtctcc gccgccagtg agcccaggtt gagtttgaga tccttggcat aatctggaag
     6901 acgatctttc agggtctgca aagacatact tgttttcctt tcagtagttc aaaatacagc
     6961 gtcaatattc ggctggccgc agccagccga atccaaggtc aagccgcttt caggaactcg
     7021 tcgcctttct tccagttgca gggacagagt tcatccgttt gcagggcatc cagaacgcgc
     7081 aatacttctt caggattgcg accgacgttc atgtcattta ccgaaacaaa tcggatgatg
     7141 ccctcgggat caacgatgaa agtcgcgcgc aaagccacgc cttcggtctt gtgcagtacg
     7201 cccagtgcgg acgacaactc gcgcttgatg tcagccagca tcgggaaggg aaggttcttc
     7261 agatccggat ggttattccg ccatgccaga tgcacgaatt cgctgtcgat cgaaacgccg
     7321 tagataacgg catcgcgatc attgaagtcc ttattcagct tgccaaagcc cgcaatctcg
     7381 gtggggcaga caaacgtgaa gtcctttggc cagaagaaca gcactttcca tttgccggga
     7441 ttggtagcct ggtcaattcg ctgaaatgcc ttgtccgggt cgatcgaaac gacggcgtta
     7501 aggtcaaatg caggaatgcg ttcgcctacg gttagcatag ttttctcctt tgggttgtct
     7561 gtcgagtatg tttccacgcc gctagtttgt gccaaacgcc tcgatcgata aagcgagaaa
     7621 tataagttat attgatcgat aaatacgatc atgggaaacg cgcatggcag cattaccctc
     7681 gttgcggcaa ctgggctatc tggtggaagt cgccgaccgg ctgaattttc gcgccgcggc
     7741 ggaggcgcgc ttcgtgactc aatcgacgct gtccgctggc atcaaggagc tggagtcgtt
     7801 gctcggcgtg gaactggttg aacgggatcc gcgcggcgta cgcctgacgc cggcaggcga
     7861 agaggtgacg cggcgtgccc gcggcctgct cgcatcagcc aacgatttgg tggaatcggc
     7921 gcgtggcgcc ggcgtgccgc tcggtggcgt ctttcggatg ggtgtcattc ctacaatagc
     7981 gcctttcctg cttactggca tgatgaccga tctacgagcg gagtatccgg acctgaaact
     8041 ccacctgcgt gaggacatca ccgataggct gctcgagaaa ctgcgcgcgg gcgaactgga
     8101 cgccgcattg attgctttgc cttatgcaat ctccgaactg atcgcaatca aattgttccg
     8161 tgacgaattc tggttcgttg cgcatgaaga cgatcctcac gccaaaaaga aagaaatcct
     8221 ggttcgcgac cttgagccgg gcagcgtcgt tttgctggaa gaggggcact gcttgcgaga
     8281 ccatgcgatc gcagcgtgcg gcgcgcgcgg cgcgaaggca aagggactgc tggaggcgac
     8341 cagtctcacc acgctgattc agatggtgga gggcgggctg ggtgttacgc tgcttccgga
     8401 attgatgata aaggcgggcg tcttgaaggg aacccatttg atcgccaggc cattttccac
     8461 cgacattcct gcccgaactg tcgcactggt ggcacgccca gccagcgcca gacgagcggg
     8521 agcggacttg cttgcggatt tcttccaacg gcattaccgg cgccagtcaa aagtgcggac
     8581 agcatcgcgg agcaacaaac gctgaccacg tgggacggtg agcgacttgc gcgcttcctt
     8641 cagtagggat aattctgcgt atttacttcc gctttctcgc cgcccgagca gaatcttcca
     8701 gctgccgcag gttcggcatc cagccccagc cgaatgcatt gataatcgta ttgagcatgc
     8761 ccagcccggt gcgggtgttc attggatgca gcgcgatgta ttggcacgtt tcgcaatccg
     8821 cgcgtggcag gagcgagcag gttttgctag ttttcatgga cgtaaacaaa ccggtgtacg
     8881 gctccagaaa tctcggagca agactgatct cgccgcaagg cgtaccgtca tcgtttgagc
     8941 agtgagatag aagcggacgg gcggacccgt ccgcttcaag ccagtgcgcg cgttacgact
     9001 tcgatggtgt ctttcgacag tcccgcagcg ccacggatgt gttccagggc cgcttgcgcg
     9061 cgtgtctgcc gcgcggcgtc aaattttttc cagcgatcaa aagcgcgtgc caaacgagcg
     9121 gcaacctgcg gattcagtcg atcgatggcg atgatctgat cggccgcaaa ggaataaccg
     9181 gcaccgtcgg gcgcatggaa acggacctga ttgccatggc aaaaactgcc gatgaccgaa
     9241 cggacccgat ttgggttctt gaggctgaac gcaggatgct gtatcagcct tttcacaacg
     9301 gaaagcgtct cgggcaaacg tgaagtcgct tggacggcga accacttgtc gatgacgagc
     9361 ggctcatgtt gccatttttc gtagaacagc cgtagtgctg cttcgcgttc tgttgcctcg
     9421 gtatttgcca gagccgtcag ggcggccatg gcatcggtca tgttgtctgc ggtatcaaat
     9481 tgcgtaaagc acagcgcgcg tatttccgca tcgtccagct ccatcaaata accaagacaa
     9541 agattccgca gcgcgcgcag ccccgcggat cgcgcatccg ggctgtaggg ccgcggagcg
     9601 ctggagctac ggtagaaagc cagcaattct gtcttcagtg cagcagcgat atggtgacgc
     9661 agtgaccggc gtaccaggtg gatggcgtcc gggtcgatct cgtctacctg ttcggcaatg
     9721 tatccttccg agggcaacgc cagcgcttcc gctgcgaaag caggatcttt tggcgcatcg
     9781 gccagcacgc gggcaaaagc tgtggcgaag ctgcctggaa attcagcagc ccgtcccgcg
     9841 cgcgcgccgt tcagcgcgcg caacagcaag tccagcgcga ggcgctgtcc agcctcccag
     9901 cgattgaaag catccgcatc gtgtgccatc aggtgcgtga gttcggcggc ggagaaatcg
     9961 aaacgcaaat ttaccggcgc agaaaatcct cgcagcaagg acggtaccgg tcgctgcgcg
    10021 atattgacga attcaaattg ttcttccggc ttcgtaagcg acaacacgcg cgtactgcca
    10081 tggctttgtg cctcacccgc cagctgcaat ggtacttcgc tgccatccgg cgcaaccagg
    10141 cccagcgcaa tcggtatatg caatggtttc ttgtgcggct gtccgggggt aggagggcag
    10201 gattgcttca cggtcaatgt gtagcggcac ccaatggcgt cgtagcgggc tgcgatttcc
    10261 agaagcggcg taccagcctg ctcgtaccaa cgcttgaact gcgcaagatc aacgccggac
    10321 gcgtcctgca tggcctgcac aaattcctcg gtgcggacag cttggccgtc atggcgttca
    10381 aagtagagat ccataccttt gcggaaacta agcgctccaa tcagcgtatg gatcatgcgt
    10441 acaacttcgg cccccttctc gtacaccgtg gcagtgtaga aattgctgat ctccatgtac
    10501 gactccggac gcaccgggtg cgccattggt ccggcatcct cggggaactg cgcactgcgc
    10561 agcgacctga cttcctggat gcgttgcagc gggcgcgaat aggtgtcggc gccgtactcc
    10621 tggtcgcgga atacggtcaa accttctttc agcgaaagct ggaaccaatc ccggcaggta
    10681 acgcgattgc cggtccagtt atggaaatat tcgtgagcta ccacccgatc gaggaacatg
    10741 aagtcggtat cggtggcggt gtccgcgcgc gcaaggacgt atttggtatt gaatatgttg
    10801 agtcccttgt tctccattgc tcccatgttg aagtcgccga ccgcgacgat catgtactgg
    10861 tccagatcga gctccagtcc aaatacttcc tcgtcccatt tcattgcacg cttcaatgcc
    10921 tgcatggcga aactgcactg gtcgagtttg cccggctcga caaacactga gagcgtaacg
    10981 cggcggcccg acctggtttg gaaggagtca tccagccgat ccagcttggc ggccaccagc
    11041 gcaaacaggt acgacggctt gggaaagggg tccacccacc gggcccaatg acggccgcct
    11101 gcttcttcac cctgcgcgat ctgattgccg ttcgatagca gaaagggaaa ggccgcttta
    11161 tccgcgtgaa cggtagtggt gtatcgggcc attacgtctg gacggtcggg aaagaatgtg
    11221 atgcggcgaa agccctcggc ctcgcattgc gtgaaatacc cgtccctcga tgcatacaac
    11281 cccatcagtt ttgtgttctg cttcggcctg atgcgaatcc tcgtagtcaa ggtgaatcga
    11341 tctgggacat tgggaatctc cagacactcg ttcgacaggg tgaagtcccg gggaccaagc
    11401 gaatgtccat caagtagcac ctccagcagt tcaagctcat caccgtccag cttgagtgct
    11461 gccatggcat tgtccgctgc cgcgtttctc gcaatggcca gttttgcact gaccaccgca
    11521 tagtcatcct gcagatcgac gtccaggtct aaagtggaca cgagaaaagc cggtgccgtg
    11581 tagtctttca aaaaaatggt tttttgagtg gacgtaagca tgatcgcgat gggcgagcgg
    11641 ctgatgattg accacgaatt ttaccgcggc gttccttccg tcgatgcgca ggcaaaaaaa
    11701 gggcgccgtg aggcgcccaa caggggaggc aattacaata atcagtgaac tgagctgtag
    11761 tagtgacgag gccgtttgta aactggctgc tcccaagcgg gcacatgtcg gtgttcgtac
    11821 attacacggt ccacaggagg cgctagatag gccggcgggc ccggcctggc aactgttatt
    11881 tcgaggggca aacgcgggcc cggatcgtag ggcagcatga tcgtggaata acgaccgttg
    11941 tagtcgtaag tgacctggta acccgttaat acctggtgat agctttccac ggtctggcag
    12001 tgctgcaccg gacggttgac cacgacggca ttgccgtccc ggtttgcaag gtggtcacct
    12061 atagcggctc caatgatggc tcccactacc gtcgacgcga cccgaccatt gccacgcccg
    12121 attgcgttgc ccgcgattcc gccggcgacg ccgcccagta tcaagccact gggcgatcgc
    12181 cgttcttcgt agctggttac cgattcggtc cagcactgtt gttgcggctg gcggacagtc
    12241 tgataaaccg gcacgcttga gaccaccggc gcgctatcca tgtaatcggc cgccgcgtag
    12301 ttgctggcga atgccggagc agccatcaca atcaggacgg aagaaaaaga agcagcgatg
    12361 ttgatattca tagtcatggt catcctctcg ttgtcccaac ccccat
//
LOCUS       DATMZQ010000136        11739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1143732, whole genome shotgun sequence.
ACCESSION   DATMZQ010000136 DATMZQ010000000
VERSION     DATMZQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143732"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VN496_04370"
     CDS             complement(<1..189)
                     /locus_tag="VN496_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16213.1"
                     /translation="MKRTLASLLVLLISAGSFATVAVAEEETTPAVSDTPAAMSDTAA
                     TEQPAADSATATDSDGTIM"
     gene            496..1662
                     /locus_tag="VN496_04375"
     CDS             496..1662
                     /locus_tag="VN496_04375"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HXF16214.1"
                     /translation="MATVTLDPIDRPHSTLSLMQTALSSVQRLKLYYEKNEHAFDIAA
                     FIGGFMFDIAMLDRIDAWSTIVQQAVYLTLIAAILLQLFFAQDKPAPALQELPRLKRW
                     YHEYRTAFVHFLLGTLLNLYTLFFFKSSSLFVSAAFMVILVLLLIANESKRLKQQGLS
                     FKFALFSLCLLSFCASIVPVFAGSVGLFVFLLSMLAACVPIAVINRRIRIHAPERQRQ
                     ARRQILVPFGLVLLLFLSLYLFRLIPPVPLSIPFIGVYHNVQRTDDTFQLSHERPFWE
                     FWHHGDQNFQAQPGDKLYVYFRIFSPGRFADQVLMRWYWKDAAGRWLLQDSIPIKITG
                     GREQGFRGYGMKSNYQPGDWKLQVDTTDEREIGRIYFDVAETLPAPREFRTDIQ"
     gene            complement(1734..2348)
                     /locus_tag="VN496_04380"
     CDS             complement(1734..2348)
                     /locus_tag="VN496_04380"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXF16215.1"
                     /translation="MLPPFTQAVIIANVVVYLLETVAGDALFQWFALWPGAGPGLASI
                     PILTFWQLVTYSFLHGGLTHLAFNMLALWMFAGELERVWGPRRVALAYFASVVCGAVA
                     QLLIGGLFGPEGGPVIGASAGVFGILLAYALVFPNRTIMPLIPPIPMPARVFVLIYAV
                     LELVLGVTGTQASVAHFAHLGGLLGGWLVYRYGRARPGNWRGLR"
     gene            2521..3924
                     /locus_tag="VN496_04385"
     CDS             2521..3924
                     /locus_tag="VN496_04385"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF16216.1"
                     /translation="MKNKIVLLIILSAQYAIAVAATPYQRPAGIADLKEPAIVAGYRA
                     LFTCSAHFFAGRPLDDIKKVELVDVEGLGYPDPVIDEQRKLVSATDISGTIVRVAAFR
                     DSMGCTVLPPHWQTGDIPRLPNVQYAAAPDVSDLPFPAGDRTELQPNGVEPRYASLAP
                     ILDRAFDGTSYGESGGVVTTAVIIMKRGKIVAERYRLGFGIHSGYRTWSTAKSISAAL
                     LAIAAKQGILDLDAPLSIPEWNYPGDPRQAITHKQLLWMSSGLFSGGDNTYAVYFGGE
                     DVVSAVTTTPLEVAPGTRWKYANNDTLLLLRELRHKLGDDLKYLRFPYDQLLHPLGMY
                     HTRMEVDHLGNFVGSSQTYTTARDLARFGLLLSYDGMWKGKRLLPEGWVKFSSTPAPT
                     RPPVAGQWGYGAQFWLLDQMRGVPPGTFTTAGNKGQFVTIVPGHDLVIVRTGVDPIGR
                     RWAQDRLVADVVPVLGK"
     gene            4023..5600
                     /gene="gshA"
                     /locus_tag="VN496_04390"
     CDS             4023..5600
                     /gene="gshA"
                     /locus_tag="VN496_04390"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HXF16217.1"
                     /translation="MNQVQERLRAFTPHVLQGIRRGIEKESLRVRHNGKLAMTPHPLS
                     LGSALTHPHITTDFSESQLELITGVHTQIEACLEELTRIHQVVYRSLGDDMLWCASMP
                     CGLPGDHEIPIGRYGSSNVGRMKTIYRTGLSHRYGRRMQMISGIHYNFSLSESSWPVA
                     QLGQPNDAYFALIRNFRRNAWLLFYLFGASPAVCGSFVEGRSHELRELAPGTYHLPYA
                     TSLRMGRLGYQSDAQSKLAVSYNNLDNYGESLFEALTEPYPPYEAIGIRDGDEYRQLS
                     TSLLQIENEFYGKIRAKRVIRPGERPLHALRERGVEYVEVRLMDLDPFSPIGIGAQTI
                     RLLDVFLLHCLLHESPPDSPADIGEVRRNQNRVAARGREPGLRLERRGQEVALSDWGA
                     QLAGECEPIAAALDAAHGTTAYADAVLAARRALDDPELVPSARVLSAMKKDHDGSYVR
                     FTLEQSLHHAAELKRLPVSPEVEQHFARLAKESIEEQRQIEAADTLPFEQYRRQYLAP
                     ERLNIPSRRKPETSLPD"
     gene            5686..5958
                     /locus_tag="VN496_04395"
     CDS             5686..5958
                     /locus_tag="VN496_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16218.1"
                     /translation="MKLWIFDSQASQEDIEAGCQAATALIEKRGLSVERAYDAVMKRS
                     SRARFDQRAAKAWDDAEDEALRVAYHGAEPDDEPVLGPFKTPDRHA"
     gene            complement(5997..6665)
                     /locus_tag="VN496_04400"
     CDS             complement(5997..6665)
                     /locus_tag="VN496_04400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXF16219.1"
                     /translation="MKIMLFCFSALLSSGWPYVHAAELANDEQKELYALGVAISQSLG
                     DFALSESDLEIVKAGLSDGVLKRTLKVDMQTFKPKLQQLAQSRAGVVAEREKKAGAGF
                     LAKAAAEPGAKKTASGAIVTTVKEGKGPSPKASDTVKVHYQGTLVDGTVFDSSLQRGQ
                     PATFPLANVIKCWTEGVQEIKVGGKSRLVCPANIAYGDRGSPPNIKPGATLVFEVELL
                     EIVK"
     gene            complement(6804..7097)
                     /locus_tag="VN496_04405"
     CDS             complement(6804..7097)
                     /locus_tag="VN496_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16220.1"
                     /translation="MVKTPAVVAFISREDYADFRAVCVDGDSMPGDYKVFLKTYNEEI
                     GAMRASGVSPTQMNIKPGELARWCKAKGLAVDAKGRTAYADFIFGGLNIKRSG"
     gene            complement(7162..8751)
                     /locus_tag="VN496_04410"
     CDS             complement(7162..8751)
                     /locus_tag="VN496_04410"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HXF16221.1"
                     /translation="MQALHFDNRFVRELPADPETGPRRRQVHRALYSRVDPTPVAQPK
                     LIAYSPEVAVLLGIDEREIESQKFADVFAGNALIEGMQPYAANYGGHQFGQWAGQLGD
                     GRAITLGEIVTPAGQRWELQLKGAGKTPYSRTADGRAVLRSSIREFLCSEAMHYLGVP
                     TTRALSLVATGEPVVRDMFYDGHPKEEPGAIVCRVAPSFIRFGNFELPTSRDDVDLLN
                     KLIDFTIRRDFPELVPQQHAGDNGGQLSEPVRAEWFAQVCERTARMVAHWMRVGFVHG
                     VMNTDNMSILGLTIDYGPYGWIDDFDLDWTPNTTDAHGRRYRFGQQPQVAYWNLGQFA
                     NALVPSYSSVDSLHAGLQRFVEAYRAAERGNIAAKLGLRECLDEDEILMRELQLLLQK
                     SEIDMTLFFRALGEIDVDAPDLAPLEPAFYDPVKRQEAVPALESWLARYAARVRRDAR
                     AHSERRASMQAVNPRYVLRNYLAQQAIDRAEQGDNGGVRELLDVLRHPYEDQPGRSQF
                     AERRPDWARNRAGCSMLSCSS"
     gene            9014..10243
                     /locus_tag="VN496_04415"
     CDS             9014..10243
                     /locus_tag="VN496_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXF16222.1"
                     /translation="MSKPLFDYAELLRPNLPPAAAPWSGFPKYNFTGGHNDADSVPVD
                     GLIAAAVAVLKREGRTLCTYGLESGPLGYRPLREFIARKLLRDAGMPCSAEEILITSG
                     SLQGLDLVNQVLVGPGDTVIVEQMTYGGAITRLQRIGANIVGVALDRDGLSSASLKSA
                     LEDLKRRGIRAKYIYTIPTVQNPTATVMSEARRGEILALSAQYGVPVFEDECYADLVW
                     QGIRPRALHAMAGNDAVIHIGSFSKSIAPAMRIGYLVAGWPLMSRILAVKSDGGSGAI
                     DQMILAEYCGSRFEAHVRTLRKALRRKLDVMIEAVREQFGAEAEFDDPAGGIFLWVKL
                     PAGVNTTRLAQVAQQAGVAINPGAEWMTDAEAGQTRLRLCFAHPAEQVIRDGVARLAE
                     VCHREFTVPARGAKEHR"
     gene            10415..11410
                     /locus_tag="VN496_04420"
     CDS             10415..11410
                     /locus_tag="VN496_04420"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXF16223.1"
                     /translation="MNIGLRRWVACATTLLATGVTNAEPPPGDYTFKFTHDRRERSYI
                     VHVPKDTLTSPWPVMLNFHGAGSSAAGQQGYSRMDETADREGFLAVYPNGTGDSGNRL
                     LTWNAGTCCGYALNNKIDDLGFVRALVDELEKHADIDRARIYATGLSNGAMMAYRIAM
                     KAPGLLAAIAPVAGALPELPAPPTPGIPIMHIHSVDDTRALYRGGLGPPYPMTNIRVR
                     HPAVEDLLARWAAYDGCPPQSVIEPPIRGSGTSASHTATRVIYGPCRDGAEVVLWKLT
                     GAGHVWPGGAGDYLARMLGSQKILGPPTDVIDANTRIWQFVSPYHSSFVRQQIPD"
     gene            complement(11419..>11739)
                     /locus_tag="VN496_04425"
     CDS             complement(11419..>11739)
                     /locus_tag="VN496_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16224.1"
                     /translation="RQHGAIVNVAAKAAIEHPAGAGAYAASKAAALALLDSLAADVRG
                     KGVRINTILPSIIDTPANRTAMPKADFAKWPKPEEIARVVLFLCSEDARVIHGAAIPV
                     YGNS"
BASE COUNT         2410 a         3535 c         3360 g         2434 t
ORIGIN      
        1 catgatcgtt ccgtcgctgt cggtggcggt cgcgctgtcg gcggcaggtt gttctgtggc
       61 cgccgtgtcg ctcatggctg cgggagtatc cgagactgcc ggggtggttt cttcttccgc
      121 gacagcgacc gtggcaaacg atccggcgga tatcagcaaa accagtaatg atgcgagtgt
      181 acgtttcata gcaggctcct ttgcgtgata gataataaaa tgatgtgcca gtcccgggcg
      241 cttgcagtct ccggctggcg tccggtgatc accagcggcc gtggattctc gcttgcgacg
      301 catttgcaac gagcatgcca agcgctcgta tccggacaat caagcgctta tgatttcggc
      361 agaaggagat ttagtcgcag atggtgcatg aatcgattcg ccgcaccaga ttgctgcacg
      421 aaaccgcgcg cctggcgttg catggcttga ccggcatcgg tccgcaaggc cattattcgc
      481 cgccaagctt caggcgtggc cactgtcacc cttgatccca tcgatcgccc gcattcgaca
      541 ctgtcactga tgcaaactgc gctttcctcc gtccagcggc tcaagctcta ttatgaaaag
      601 aacgagcacg ccttcgacat tgcagctttt atcgggggct ttatgttcga catcgccatg
      661 ctggatcgca ttgacgcatg gtccaccatc gttcagcagg cggtttatct aaccctgatc
      721 gccgccattt tgctgcaatt attttttgcg caagacaagc cggcgcccgc gttgcaagag
      781 ttgcccaggc tcaagcgctg gtatcacgaa taccgaacgg cgttcgtgca ttttctgctc
      841 ggtaccctgc tgaacctcta tacgctgttt tttttcaaga gttcgtcgct attcgtctcg
      901 gctgcgttca tggtaatcct ggtgctgttg ctgatcgcga acgaatcgaa acgtctgaaa
      961 caacagggct tgtcgttcaa gttcgcgctg ttcagccttt gcctgctgtc gttctgcgcc
     1021 agcatcgtcc cggtattcgc gggttccgtc gggctgttcg tgtttctgct gtccatgctg
     1081 gcggcgtgcg tgccgattgc ggtcatcaac aggcgcattc gcatccatgc accggaacgc
     1141 cagcggcagg cgaggcgaca gatccttgtg cctttcggcc tggtgctgct gctgtttcta
     1201 tcgctgtatt tgttcaggct gatcccgccc gttccactat cgattccatt catcggcgtc
     1261 tatcacaacg ttcagcgcac ggacgacaca ttccagctca gccatgaacg tccattctgg
     1321 gagttctggc accatggcga ccagaatttt caggcgcagc cgggcgacaa gctctatgtg
     1381 tacttccgca ttttttcgcc cggtcgcttt gccgaccagg tgctgatgcg ctggtactgg
     1441 aaagatgccg caggccgctg gttactgcag gattccattc ccatcaaaat caccggcgga
     1501 cgcgagcagg gattccgtgg ctatgggatg aaatcgaatt accagcccgg cgactggaag
     1561 ctgcaggtgg ataccaccga tgaacgggaa atcggacgca tctacttcga cgtcgctgaa
     1621 acgttgccgg caccgcggga gttccggacc gacattcaat agtcagcgca cctgtcgatt
     1681 gcccttggct tttgtcatgg atgaaggcgc tgatgcatgg ccggggctcg ccgtcagcgc
     1741 agtccgcgcc agtttccggg acgtgcgcgc ccatagcgat agaccagcca cccacccagc
     1801 aatccgccga ggtgcgcaaa atgcgcaacg ctcgcctggg ttccagtcac acccagcacg
     1861 agttcgagta ctgcataaat cagcacaaat acccgcgccg gcatgggaat cggcgggatc
     1921 agcggcatga tcgtgcgatt gggaaagacc aatgcgtaag ccagcaaaat gccaaataca
     1981 ccggccgatg cgcctatcac gggaccgcct tcgggaccaa acaatccgcc gatcaggagc
     2041 tgtgcaacgg ccccgcacac gacgctggca aagtaggcga gcgctacgcg gcgcggaccc
     2101 cacaccctct ccaattcacc ggcgaacatc cataacgcca acatattaaa tgccaggtgc
     2161 gtcaggccgc catgcagaaa actgtaagtg accagctgcc agaacgtcag gatcggaatg
     2221 cttgcaaggc caggcccggc accgggccat agggcgaacc actgaaacag cgcatcgccc
     2281 gccaccgttt ccagcagata gacgacgacg ttggcgatga tgacggcttg cgtaaaaggt
     2341 ggaagcacgg cggtccttgt ttattttttg gtctgattcg acccggccac gaataaaaaa
     2401 ttctttgcat ggtacgcttt cgtccctgca acatgacagc gctagggcgg cggtcccgct
     2461 gcggctttag aactgtacct ttgatttaat cacgatcccg aaaaataaag gcgtcgcaac
     2521 atgaaaaaca agatcgtcct gttgatcatc ctgtccgctc aatatgccat cgccgttgcg
     2581 gcaacgccct accagcggcc agccggcata gcggatctca aagagccggc cattgtcgcg
     2641 ggatatcgtg cactgttcac ctgctccgca catttttttg ccgggcgtcc gctggatgac
     2701 atcaaaaagg tcgagctggt cgatgtagaa gggctcggtt atcctgaccc agtcatcgac
     2761 gagcagcgca aactggtctc cgccacagac atttccggaa cgattgtgcg cgtggctgcg
     2821 ttccgcgaca gcatgggctg cacggtactg ccgccgcatt ggcagaccgg cgacatcccg
     2881 cgcttgccga atgtgcaata tgctgccgca cctgatgtgt ccgatttgcc ttttcccgca
     2941 ggggatcgca cagagctgca gcccaatgga gtcgaaccgc ggtacgcgag tctggcgccg
     3001 atactggatc gcgcattcga cggcacgagt tacggagaaa gcggcggggt cgtcaccacg
     3061 gcggttatta tcatgaagcg cggcaaaatc gtcgctgaac gctaccgcct tggattcggc
     3121 atccactccg gctatcgcac gtggtccacc gcaaagagta tttcggccgc tttgcttgct
     3181 atcgccgcca aacagggcat tctggacctt gacgcgccgc tcagcatccc ggaatggaac
     3241 tatcccggcg atccgcgcca ggccatcacg cataagcaac tgctgtggat gtccagcgga
     3301 ctcttcagcg gcggcgacaa tacctacgct gtgtacttcg gaggagagga tgtcgtcagc
     3361 gcggtcacca ccacgccgct ggaagttgca ccaggcacgc ggtggaaata tgccaacaat
     3421 gacacgttgc tgttgctgcg cgagttacga cacaaactcg gcgacgatct gaagtacctg
     3481 cgctttccgt acgaccaact gctgcacccg cttggcatgt atcacacgcg catggaggtg
     3541 gatcacctgg gcaattttgt cggctcgagc caaacctata ccacggcacg tgacctcgcg
     3601 cgcttcggcc tgctgttgtc atacgacggt atgtggaaag gcaaaagatt gctgcccgaa
     3661 gggtgggtga aattttccag cacgccggca ccaaccaggc cgccggtggc cggtcaatgg
     3721 ggctacgggg cacagttctg gctgctggac cagatgcgag gcgtgccgcc gggtaccttc
     3781 accactgcgg gcaacaaggg ccagtttgta accatagttc ccggtcacga tctggtaatc
     3841 gtccgcaccg gggtcgatcc gatcggcagg cgttgggcgc aggaccggct ggtagcggac
     3901 gtcgttccag tgttgggcaa ataaatcttc cgcgacattg ctccagcgcg gatatcgtgc
     3961 ccttactttc tgttacctta cgccctgctc catcagccca tctccacccg aggcattccg
     4021 ttttgaacca agtccaggaa cgactgcgcg cttttacgcc ccacgtgctg caaggtattc
     4081 gccgtggcat cgagaaagaa agcttgcggg tgcgccacaa tggcaagctg gcgatgactc
     4141 cgcacccgtt gtcgctcggc tcggcgctga cccatcccca tatcaccacc gacttcagcg
     4201 aatcgcaact cgagctaatc accggcgtgc atacgcagat cgaagcgtgc ctggaggaac
     4261 tcacgcgaat tcaccaggtc gtctatcgct cgctgggcga tgacatgctt tggtgcgcga
     4321 gcatgccatg cgggttgcca ggcgatcacg agattccgat cggccgctac ggcagctcca
     4381 atgtcggccg catgaagacg atctatcgca ccggcttgtc gcatcgctac ggccggcgca
     4441 tgcaaatgat ctcgggcatc cactacaact tttcgctatc cgagagcagc tggccggtgg
     4501 cgcagctggg acagccgaat gatgcgtact tcgcgctgat ccgtaacttc cggcgcaatg
     4561 catggctgct gttctacctg tttggcgcat cgcctgcggt gtgcggcagt ttcgtcgaag
     4621 ggcgctcgca tgaattgcgc gagcttgcgc ccggcaccta tcacctgccc tatgccacct
     4681 cgctgcgaat gggaaggctc ggctaccaga gcgacgcgca gtccaagctt gcggtaagtt
     4741 ataacaacct ggataactat ggcgaatcgc tgttcgaggc gctcacggaa ccgtatccgc
     4801 cgtacgaagc aatcggtatc cgggacggcg atgaataccg ccagctcagc accagcctgc
     4861 tgcagattga aaacgagttc tacggcaaga tccgcgcgaa gcgcgtcata cgtccaggag
     4921 aacggccgct gcacgcattg cgcgagcgcg gcgtggaata tgtcgaagtc cgtttgatgg
     4981 atctggatcc cttttccccg atcggcatag gcgcgcaaac catacggctg cttgacgtct
     5041 tcctgctgca ttgtttgctg catgaaagtc ccccggacag tcccgcggat ataggagaag
     5101 ttcgccgcaa ccagaaccgc gtcgccgccc gcggccgtga gcccggtctg cgtctcgaaa
     5161 ggcgcggcca ggaagtcgcg ttatcagact ggggcgcaca gctggcaggc gaatgcgagc
     5221 ccatcgcggc tgccctggat gccgcgcacg gtacaacagc ttatgctgat gctgtgctgg
     5281 ctgcgcgccg cgcgctggac gatcccgagt tagtgccgtc tgcgcgggta ctcagtgcaa
     5341 tgaaaaagga tcacgacggg tcgtacgtgc gctttacgct cgagcagtct ctgcaccacg
     5401 ccgccgaatt gaagcggttg ccggtttcac cggaagttga acagcatttc gcccgactgg
     5461 ccaaggagtc gatcgaggaa caacgacaga tcgaagcggc cgacacgctg ccgtttgaac
     5521 agtatcggcg gcaatatctt gcaccggaac gattgaacat tccgagccgg cgcaaacctg
     5581 aaacatcgct accggactga cttgaatttt ttcatgcgcg ccttgtccca atgcgcattc
     5641 atgccgataa tttcaccccg cacaacgaca gcgcaggact aaccgatgaa actctggatt
     5701 ttcgattccc aggcatccca ggaagatatt gaagcgggct gccaggccgc caccgcactc
     5761 atcgaaaaac gcggcctcag cgtcgagcgc gcctacgatg cagtgatgaa gcgcagcagt
     5821 cgagctcgct tcgaccagcg cgcggcaaag gcgtgggacg atgcggagga tgaagcattg
     5881 cgcgtcgcgt accacggcgc cgagccggat gatgagccgg tgcttggacc gttcaagacg
     5941 cccgatcgcc atgcgtagca gacggggcgc gccataggca tacaacggcg cccggtttat
     6001 ttcacgattt ccagcagttc gacttcgaac acgagcgtgg cgccaggctt gatattaggc
     6061 ggtgatccgc gatcgccgta agcgatgttg gccggacaca ccagccggct tttgccacca
     6121 accttgattt cctgcacgcc ttccgtccag cacttgatga cattcgccaa cgggaacgtg
     6181 gccggctgtc cacgctgcag tgaactgtcg aacaccgttc cgtccaccag tgtgccctga
     6241 taatgcactt tgaccgtgtc cgaagccttg ggcgaggggc ctttgccttc tttcactgtc
     6301 gtcacgatgg ctccggatgc cgtcttcttt gcgccaggct cggccgcggc cttggccagg
     6361 aaacctgcac cggccttctt ttcccgttct gccacgacgc cggcccgcga ctgcgcaagt
     6421 tgttgcagct ttggcttgaa ggtttgcatg tcgactttga gcgtgcgttt cagaacacca
     6481 tccgataagc ccgccttgac gatttccaga tctgattcgc tcaaagcgaa atcgccgagg
     6541 gattggctga tcgcaacacc aagcgcgtat aactccttct gctcgtcgtt cgcaagctcg
     6601 gcggcgtgca cgtacggcca tccagaactc aacaacgcgg agaagcagaa caacatgatt
     6661 ttcatggtcg accttttgaa aagaaaatgc tcgcaatggt actggcgttg ccttgcaagt
     6721 tggagtgact acacgttgcc ccgaactgaa caatcgggga aagagcggag gtaactaaac
     6781 ccgcgggtcg ttagccagcg tattcagccc gaccttttga tattcagccc gccaaaaatg
     6841 aagtccgcgt aggcggtgcg gcccttggca tcaacggcta gccccttcgc cttgcaccag
     6901 cgcgccaact ctcccggctt gatgttcatt tgtgtcggtg aaacgccgct ggcacgcatt
     6961 gcgccaatct cttcgttata agtcttcagg aataccttgt aatcgcccgg catgctgtcc
     7021 ccatcgacgc aaaccgcgcg aaagtcggcg taatcttctc tcgatataaa cgcgacgact
     7081 gcaggtgttt taaccatgat cgttctccat gtaatgcttt ataaacttcg atcgctgcca
     7141 cgcttgggcc gaattgccgg cttacgagct gcaggacaac atcgagcatc ctgcccggtt
     7201 gcgcgcccaa tccgggcggc gctcggcaaa ttggctgcgg cctggctggt cctcgtaggg
     7261 atggcgtaat acgtcaagca gttcacgaac gccgccatta tccccctgct ccgcgcgatc
     7321 gatggcctgt tgcgcaaggt aattgcgcaa cacgtagcgc ggattgactg cttgcatgct
     7381 cgcacgtcgt tccgaatgcg cgcgcgcatc gcgccgcacg cgcgctgcgt agcgcgccag
     7441 ccacgattcc agcgccggaa cggcctcctg gcgcttgacc gggtcgtaaa acgctggctc
     7501 caggggcgca aggtctggcg catcgacatc aatctcgccc agcgcccgaa aaaacaacgt
     7561 catgtcgatt tcacttttct gcagcaacag ttgcagctcc cgcatgagta tttcgtcttc
     7621 gtccagacac tcccggagtc cgagttttgc cgcgatgttg ccgcgctccg ccgcgcggta
     7681 agcctcgacg aagcgttgca atccagcatg caacgaatcg acgctggagt aggagggaac
     7741 cagcgcattg gcaaattggc ccaggttcca gtacgcgacc tgcggctgct gcccgaaccg
     7801 gtaacgccgt ccgtgcgcgt ccgttgtatt aggagtccag tccagatcga aatcatcgat
     7861 ccagccgtaa ggaccgtaat cgatggtcag ccctaggatc gacatgttgt cggtattcat
     7921 cacgccgtgc acgaagccga cgcgcatcca gtgagccacc atgcgtgcag tgcgctcgca
     7981 tacctgcgca aaccattccg cacgcaccgg ctcgctcagc tggccgccat tatcgcccgc
     8041 atgctgctgt ggaaccagct ccggaaaatc acgacggatt gtaaaatcga tcaacttgtt
     8101 cagcagatcg acatcgtcgc gcgatgtggg caattcaaaa ttgccaaaac ggatgaacga
     8161 cggcgccaca cggcacacaa tggcacccgg ctcttccttg ggatggccat catagaacat
     8221 gtcgcgcaca actggttcgc cggtcgctac caggctcaat gctcgggtag tcggtacacc
     8281 aagataatgc atcgcttcac tgcacaggaa ttcccggatc gaagagcgta acaccgcgcg
     8341 gccatccgcc gtgcgggaat agggcgtttt gccagcacct ttgagctgca actcccagcg
     8401 ctgtccggcc ggggtgacga tttctccgag cgtaattgcg cggccatcac cgagctggcc
     8461 ggcccactga ccgaactgat ggccgccgta atttgcggca taaggttgca tgccttcgat
     8521 gagcgcgttt cccgcaaaaa catctgcaaa tttttgtgac tctatttccc gctcgtcgat
     8581 gccgagcagt accgcaactt ccggtgaata ggcaatgagt ttcggctgcg ccaccggggt
     8641 aggatcaacg cgcgagtaca acgcccgatg tacttgccga cgccgcggtc ccgtttccgg
     8701 atcggcaggg agttcacgta cgaagcgatt gtcgaaatgc agggcttgca tgctgttggg
     8761 agagagcaaa agcagataag ttcagcgtac gagtgcccat tgtaacgggt tcacatgtga
     8821 cttatctcac caatacactc gcccaaaccc cctgccagat agcaaataac ggatcaggta
     8881 acagcttgag ggagaattgc acagtgtgcc gggtacgcag accgcatggc ggttcagcgt
     8941 acgatatgca cattattttt aaaatgctct atcgcacatg cgtgttgcgt gctgcgtgca
     9001 tccggagaac caaatgagca aaccgctctt cgactacgcc gaactgctgc gtccgaattt
     9061 gccacctgcc gcggcgccct ggagcggatt cccgaaatac aacttcaccg gcggacacaa
     9121 tgatgccgat agcgtgccgg tcgatgggct catcgcagcc gcggtcgccg tgctgaagcg
     9181 ggaaggcagg acgctgtgca catatggcct ggaaagtggg ccgctcggtt atcgcccgtt
     9241 gcgggaattc atcgcgcgca agcttttgcg tgatgcaggc atgccttgtt ctgcggaaga
     9301 aatcctgatc acgtccggat cgctgcaggg actcgatttg gtcaaccagg tattggtggg
     9361 accaggcgac acggttattg ttgaacaaat gacttatggc ggcgccatca cgcggctgca
     9421 aaggatcgga gcaaatattg tcggtgtcgc gctagatcgt gacggcctca gcagcgcgtc
     9481 gctcaaatcc gcgcttgaag atttgaaacg ccgcgggatc agggcaaagt atatctatac
     9541 cattccgacc gtgcaaaacc cgaccgccac cgtcatgagc gaagcgcgaa gaggcgagat
     9601 actggcgctg tccgcccagt acggcgtgcc ggtcttcgag gatgagtgct atgcagacct
     9661 ggtgtggcaa ggcatacgcc cacgggcctt gcacgccatg gcaggcaatg atgctgtcat
     9721 tcacatcggt tcattttcca aatccatcgc tcccgcgatg cggatcggtt atctggtggc
     9781 cggatggccg ctcatgagtc gcattctggc tgtcaaaagc gatggaggct ctggcgccat
     9841 cgatcagatg atcctcgctg aatattgcgg tagccggttc gaagcgcacg ttcgcacgct
     9901 gcgcaaggca ctacgccgaa aactcgatgt catgatcgaa gcggtgcgcg agcaattcgg
     9961 agctgaagcc gaattcgacg atccggcggg cggaattttt ctgtgggtga agcttccggc
    10021 gggcgtcaat accacgcgcc ttgctcaagt agctcagcaa gctggtgtgg ccatcaatcc
    10081 cggtgccgag tggatgaccg acgcagaagc cggccagacc cgcttgcgcc tttgcttcgc
    10141 gcatcctgcg gaacaggtca tccgcgacgg tgtggccagg ctggccgaag tttgccatcg
    10201 cgagttcacc gtgccggcgc gcggcgcgaa agaacatcgt tagtcacact ttttttcttt
    10261 aatttgccgg ttgcagagac cgggacatac tctggcttgt cccatgacaa attgaaatct
    10321 ggttttcagt ctgaagcttg attttgttgt catcggcgta atatttcctc acggctatgc
    10381 ttcattaagg ccgcaccgtg aggagagatt gcacatgaac atcgggctgc gcagatgggt
    10441 cgcgtgcgcc acaacgctcc ttgcgaccgg cgtcacgaac gcggaacctc ctccgggcga
    10501 ctacacattc aagttcacgc acgatcgccg cgaacgcagt tacatcgtgc atgttccgaa
    10561 ggacacgctg accagtccgt ggccagtaat gctcaatttc cacggcgcgg gcagcagtgc
    10621 cgccggtcag cagggctatt cgcgcatgga cgagacagcg gatcgcgaag gctttctggc
    10681 cgtctatccg aacggcaccg gcgattccgg caaccgcctg ctgacctgga atgcaggaac
    10741 gtgttgcggc tatgcgctca acaacaaaat cgatgacctc ggattcgtgc gggcgctggt
    10801 tgacgaactg gaaaaacatg ctgacataga ccgggcacgc atctatgcga ccggcctttc
    10861 caacggtgcg atgatggcct atcggatcgc gatgaaagcg cctggactgt tagcggccat
    10921 cgcgcccgtg gccggcgctc tgccggaact ccccgcaccg ccaacgccgg gtattcccat
    10981 catgcatatt cacagcgtcg acgatacgcg cgcgttgtat cggggcgggc ttgggccgcc
    11041 gtatccaatg accaacattc gcgtcaggca tcccgcggtg gaggatctgc tcgcccgctg
    11101 ggcagcgtat gacggttgcc cgcctcagtc cgtcattgaa ccgcccattc gcggcagcgg
    11161 tacttcggct tcacacactg ccacgcgcgt gatctatggc ccatgccggg atggcgccga
    11221 agtggtgttg tggaagttga ccggcgccgg ccatgtctgg cctggcggcg cgggcgatta
    11281 cctggccaga atgctcggct cgcagaagat tctcggtcct cctaccgacg tcatagacgc
    11341 caatacgcgg atatggcaat tcgtctcccc ctaccatagc agcttcgtcc ggcagcaaat
    11401 acccgactga gctgcggctc aactgttgcc gtataccggg atggccgccc cgtgaataac
    11461 gcgcgcgtcc tcgctgcaca ggaacagaac gacgcgcgcg atttcctcgg gcttcggcca
    11521 cttcgcgaag tccgcctttg gcatcgccgt gcgattggct ggcgtatcga tgatgctcgg
    11581 cagaatcgtg ttgatgcgca cgcctttacc ccggacatcc gccgccagtg aatctagcaa
    11641 cgccaacgcg gcagctttcg aagcggcgta tgcgccggca ccggcgggat gttcaatcgc
    11701 cgccttggcc gccacgttga cgattgctcc gtgttggcg
//
LOCUS       DATMZQ010000137        17497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2127010, whole genome shotgun sequence.
ACCESSION   DATMZQ010000137 DATMZQ010000000
VERSION     DATMZQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127010"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VN496_04430"
     CDS             <1..771
                     /locus_tag="VN496_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXF16225.1"
                     /translation="LIAGMQLGTVDMAYLGVGNAGQLKGCGALNVSYLPYLFKSKRAA
                     EDGLNGPIFQELYEQCAQSAGVRIFAVGGDRSPRAIVTTRGPILKPEDLNGMRLRIPP
                     IPIFRETFEAVGVKVVPLGLNEVYMALSRGQVDGQDNGFDLTLSFRFHEVSKYWSATD
                     HCYDMGAFYVSERKWKTFTPEQKALFKRGAEEGGKLATQLGEEFDKRGLEELKKAGVN
                     YVVPDKAAFARAWANVYKSYEGKVWPDGLVQRIKSAQK"
     gene            777..1286
                     /locus_tag="VN496_04435"
     CDS             777..1286
                     /locus_tag="VN496_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXF16226.1"
                     /translation="MDFLDKALRLAERALEWLISILVAVLLVIVASTFIDRHFVTLPM
                     AAPDSYARVILVWLTFIGFALAVKNGLNIRVDLIDSRLPAKLRRLFEYVFDLTMLGLT
                     IMLGLNGWRLVIIGLDQQRLGTMMSEAWPSGAFFLTCIILVLFLLLRIALRFYGRELP
                     QHHLPLEVE"
     gene            1290..2567
                     /locus_tag="VN496_04440"
     CDS             1290..2567
                     /locus_tag="VN496_04440"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXF16227.1"
                     /translation="MLLLLFIAFMLALIIGFDVGFSMILSAWLGVLYRGDAVDPAMMP
                     LSMIAGVDTYALVQVPLFILAGELMNRGGLTMRLIDWAQAMVGRVKGSLGHVSILTNL
                     VMAGVSGSAVADAVATGKPLIPAMQREKYGEGFAGAVIAAGALLGPIIPPSIPMVVYA
                     QLSSQSVVKLFMAGIVPGLLLAAGFMAVCSVVARVRNYPSRQPVPWRGKVKATGRAGW
                     ALLMPVIIIGGIRFGLVTDTEAAAVAVIYSLIVSLFVYRGIGLGELRRLLFESGRSTA
                     VILFLLAAAGPFSWLVAESQVSQTVVQTIQGWSANPIIVLLIINAFLLLVGCIIEPLP
                     AMIIFLPVLIPLGAQLGIDPIQFGAVVVLNLMIGLMHPPIGLLLFVVSSVGKIRIGPV
                     MWEILPFLAWAIVVLILTIVYPPLTIWLPAQIK"
     gene            2671..3924
                     /gene="hemA"
                     /locus_tag="VN496_04445"
     CDS             2671..3924
                     /gene="hemA"
                     /locus_tag="VN496_04445"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXF16228.1"
                     /translation="MQLFAFGMNHHTAPLAVREQVTFHAENIDAALRDLVTHESVREA
                     AIISTCNRTEIYCSTPEPVRAIDWLARFHHLKAPALEPFLYTLPRERAVNHAFRVASG
                     LDSMVLGEPQILGQMKEAVKSAEHAGTLGTVLHRLFQSTFSVAKEVRTQTDIGARSVS
                     LAAAAVKVAERIYPSIAGQKIMFVGAGEMIELCATHFAARKPRELTFANRSVDRGVTL
                     GSRFSGNIITLNDLPEQLALHDIVVTCTASTLPILGKGMIERAIKARRHRPILMIDLA
                     VPRDVEAEVAELDDVFLYDLDDLGKIVQEGLDIRQAAVAQAEAIINTNVHAFMQWLDN
                     REVVPTIRALRDQAEQTRRHELERASRMLAKGDEPAKVLEQLSHSLTNKFLHGPTQAL
                     NGVEANEREALIAALTRLHGVKPPE"
     gene            3921..5018
                     /gene="prfA"
                     /locus_tag="VN496_04450"
     CDS             3921..5018
                     /gene="prfA"
                     /locus_tag="VN496_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXF16229.1"
                     /translation="MKPSIASKLAQLSARLEELNALLSSEDVTKDLENYRKLTREHAE
                     ISPAVELYNAYVKSAQDIAAAQDMANDPEMRELAETEISEGKKRLLELEAELQKRLLP
                     KDPSDERNIFLEIRAGTGGDESALFAGDLFRMYFRYAERLGWKVELISQSLGEMGGYK
                     EVIAKIIGFGAYSQLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADAIEDVVLN
                     PAELRVDTFRASGAGGQHINKTDSAVRITHLPTGIVVECQDDRSQHKNKAQAMAVLAA
                     RIKDKQVREQQAQQAATRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDQIMD
                     GAMDDLIHALQAEHQTELLAAMAEETFSLQA"
     gene            5015..5857
                     /gene="prmC"
                     /locus_tag="VN496_04455"
     CDS             5015..5857
                     /gene="prmC"
                     /locus_tag="VN496_04455"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXF16230.1"
                     /translation="MTPTVSVLLEEASHQLACAELVEARREAQVLLGHALGKSRAWLS
                     AHSEDQVDPSVVARFRQLVQRRLSGEPVAYIVGRKEFYGLEFRVTPDVLIPRADTETL
                     VDAALARMSAAGCDVLDLGTGSGCIAIAIAHERPAARVMAVDVSSAALSIARENALAN
                     AVDVQFLQASWFEALDGRRFDIIVSNPPYVATEDPHLQQGDLRFEPAIALAGGNDGLA
                     DIRLIVAKAPSYLRDQGWLLFEHGHTQACDSRNLLANAGFQDITHSRDIAGLVRVAGG
                     RLLS"
     gene            5942..6250
                     /gene="grxD"
                     /locus_tag="VN496_04460"
     CDS             5942..6250
                     /gene="grxD"
                     /locus_tag="VN496_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HXF16231.1"
                     /translation="MNAQDKIKQQVTGNPIVLYMKGTPDAPMCGFSAAAVQILEACGA
                     ENVATVDVLSDQDVRQGIKEYSNWPTIPQLYVNGEFVGGADIMREMYQSGELQKLFGK
                     "
     gene            6447..9683
                     /locus_tag="VN496_04465"
     CDS             6447..9683
                     /locus_tag="VN496_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF16232.1"
                     /translation="MMELVRVALRRPYTVAVLSLLVLLLGGLAVTRMVVDFFPKIDIP
                     VVFVGWNYPGLSAEEMERRVVIISERAYSTTVNGIDRIESNCIPGIGLVKVFFHQGTD
                     LGASIAQINAINGTILRILPPGITPPVIIQYDAATVPIVQMVLSSKTIPEGRIFDHAF
                     NFVRVKLFTIPGLTVPAPFGGRLRQIMVDLNPQLMTAKGISPNDVVNALQSSNVLVPA
                     GTARIGSMEYNVMMNSSPPTVDQFNLMPLRISNGVPVMLGDVAKVADGFAVQNNIVHV
                     DGRRATYLTILKHSEASSLAVVEATRALLPEIQKAAPEGMEIKLDFDQSVYVRAAIKN
                     VATEAVIASLLVSLMVLLFLGSWRNTIVVITSIPCAMAAGILGLYFTGNTINLMTLAG
                     LSLATGILVDDATVAVENIHRHRAMGQPLTVAILDGSREVALPRTMATLAICIVFIPV
                     AFLYGVSKFLFIPLALSVVFALLASYILSFTLVPMLSRQLLAGEHHEDVDDRSFGAQL
                     NRVREGTLERLQKTYGAILTIIMHHRPFVLVCLLLMGGVSVFLGRVVGTDFFPTADVG
                     ILKLHFRAPMGMRIEDTEKLVLDVQEEIRRIIPAGEISTINDMLGVPLFFNLAFVPTD
                     NISGMDADILISLKHPHQPSAYWMRELRAKLAPKFPGATMYFQNADIVTQVLNFGLPA
                     PIDVQIQDANFDRAQGYAARVRKIIEETPGAVDVRQMQVLNYPSLRVEVDRVRAARLG
                     LTQRDVASNVLTTLSSSAIVNPSYFLNPNGVNYTVAVQTPIERMSSVDDLMATPITTG
                     STPIADTTAVARLDARPGAPVTRLSNIASVKPETSYQSVSHYTVQRVLDIAANVDGRD
                     LGGVVSDIKRKIDAMTAEKDFPPTARINIRGQYQVMNSSFESLIGGLVLAVVLVYALL
                     VVLFQSWIDPFIIMMAVPGALIGILWMLALSGTTINVMSLMGAIMAVGIGVSNSILIV
                     SFANDLRVERNLGVIEAAVEAGVTRLRPVLMTALAMIIGMVPMALGLGEAGEQNAPLG
                     RAVIGGLAMATIATLFVVPVIYTFVRKNMPVLHGLDARFEAESQGHHA"
     gene            9696..10973
                     /locus_tag="VN496_04470"
     CDS             9696..10973
                     /locus_tag="VN496_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF16233.1"
                     /translation="MAAMQRRRDSFLVFYIIAFVVVAAAGASVWWFWQTKQSHLADEA
                     KARTAQVDAGPTVLVGQSVRGSPTRKISLTGELRPWKATTLYSKVGGYLSKITVDVGD
                     HVRANQLIAEVQAPELESEYKSTISEMQNRERIAQRTRDLAAKGFFSQQALDNADTDV
                     RTSRGRVDTLRSQMGYRSLYAPYAGVVTGRYVDAGALITNAANNQTSSQPVVTIADPS
                     KLRVSVYVEQVDAIYVRPGIDAEIVDTANPTQKIKAKVSRVAGELDPRTRTLLAEVDF
                     DNRTGTFIPGSFVNVSLIVPQASYIEVPAAALVVRDRKNFVAVLESGNHIKLTPIEVA
                     GTDGSAVRIINGLGEGTRVVLSLANTVPDGAKVNPAATPMASAPPAPAAAATPAAKAV
                     TANPGAPVPAVPQTPSAIATTPKTEGVNSQRPK"
     gene            11046..11357
                     /locus_tag="VN496_04475"
     CDS             11046..11357
                     /locus_tag="VN496_04475"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16234.1"
                     /translation="MMNYRGIHDMGGLPAGRVQATEHDYALWEKRVDALLVLLSGKGY
                     VTVDELRRNIETLGAEAYDKMSYYERWIYAITQTLIQRGTVTIEELGAEIAQAQAHGE
                     R"
     gene            11344..11643
                     /locus_tag="VN496_04480"
     CDS             11344..11643
                     /locus_tag="VN496_04480"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HXF16235.1"
                     /translation="MANADSIDPLFRPGDGVTVRAIYPVGHVRTPYYIRGKRGVVERL
                     CGSYGNPEELAYARSGLPRQPLYRVRFLQRDVWADYAGAAHDTVDIEIYQHWLER"
     gene            11643..12287
                     /gene="nthA"
                     /locus_tag="VN496_04485"
     CDS             11643..12287
                     /gene="nthA"
                     /locus_tag="VN496_04485"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HXF16236.1"
                     /translation="MAHDHHHSHDETPHKHPPLRPDQDDTITYWRAMEIAVRGLLIKK
                     GIVSADEVRRTVEQMDNRTPLQGAKIIAKAWVDADFRKLLLSDGNAACKALGVEEGFY
                     KLVVVENTEKVHNVIVCTLCSCYPRWLLGLPPDWYKSRNYRSRVISEPREVLREFGTQ
                     IANDTVVRVHDSTADMRYLVLPKRPAGTDGWSEDKLAALVTRDAMIGVANAQHP"
     gene            12417..13325
                     /locus_tag="VN496_04490"
     CDS             12417..13325
                     /locus_tag="VN496_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HXF16237.1"
                     /translation="MKSPNLDAIKSIAFGQPRRFFQPPAKGEVLANGERRYFIGDAIG
                     QGGFGMVYDCIDDWGNPLVAKILIPHEQSYREVRRVWQEELRKLLVMRHPNITFVYDA
                     FEYKDTFYLIIERCSFPLSRLITRLGLDADRWIPYVARDVLQALDYIHAAGYVHKDLH
                     PGNVFVTEQRRKRRLIKEQVWSFKVGDLGLTRLESDIRVFKTLLAEWMRPPEAFEPEK
                     FGAVGHQVDIYQTGLLLLALMLKEIPYFTRDEILGGIPQALAAKHESKFGPVVAKALR
                     RHVEWRTQTALEFWRELSGVAGASPP"
     gene            complement(13385..13876)
                     /locus_tag="VN496_04495"
     CDS             complement(13385..13876)
                     /locus_tag="VN496_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HXF16238.1"
                     /translation="MKATPRSIHLVMFLVCAGLIAFALYLQHSVGLEPCPMCILQRYA
                     FIVVGVIALVAALHGPALIGQRVYSGLILLFAATGAGVAIRHVYLEHNPPKIFDCGAD
                     VGFMLESFPLTEALPMIFRGTGDCTKVLWRFLGLSIAEWSLICFVLLIVAALVALTNK
                     KRY"
     gene            complement(13876..15045)
                     /locus_tag="VN496_04500"
     CDS             complement(13876..15045)
                     /locus_tag="VN496_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXF16239.1"
                     /translation="MKNEPSTQGFATRSIHAGQPPDRTTGAVMTPIFATSTFKQQSPG
                     VHTGYEYARSQNPTRHAFERCIADLENGTRAFAFASGLAASSTVLELLDSGSHIVAAD
                     DLYGGSWRLFERVRKRSMGLRTSYSDPNVTGSLQQAIQPDTKMIWVETPSNPLLKITD
                     LAEVARIGQARGILTVVDNTFASPFIQRPLDLGIDIVLHSVTKYISGHSDMVGGAVIV
                     KERGDIAEKLEYMQNAIGSILDPFSSFLALRGLKTLPLRMRQHCGNAMHLATWLKSHP
                     KLEKVIYPGLPNHAQHLLAAKQMNGFGAMISIYLKTDMNGVRRALEACKIFTLAESLG
                     GVESLIGHPVTMSHGSLPSDLRARLGITDNLVRLSVGIEDIEDLIADLDHALALI"
     gene            complement(15121..16482)
                     /locus_tag="VN496_04505"
     CDS             complement(15121..16482)
                     /locus_tag="VN496_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HXF16240.1"
                     /translation="MATNRLLDLIGNTPLVEITGMDAGRCRIFLKLESQNPGGSIKDR
                     IGRSMIEAAEADGRLKPGGTIIEATAGNTGLGLALIATLKGYKLILVVPDKMSREKIF
                     HLKAMGAEVVLTRSDVGKGHPEYYQDMAKTIADETGAFFVNQFGNPANPLAHETTTGP
                     EIWRQMHQDVDAIVCGVGSAGTITGLSRYFQKVSPRTELVLADPVGSVLADFVRTGTY
                     GEAGSWLVEGIGEDFIPPIADFSRVKKAYSISDEESATTVRELLAKEAILAGSSTGTL
                     LAAALRYCREQTTSKRVVTFVCDSGNKYLSKVFNDYWMMDQGFIKREHFGDLRDLITR
                     RHDEKATVTIGPDDKLMTAYARMKLYDISQLPVLEDGKVVGLIDEWDLLTASREDPEH
                     FKDPVRSAMTQRLETVGLKTPIPRLLETFNKGHVALVIEDGKFYGLVTRMDVLNHLRH
                     RAP"
     gene            16760..17059
                     /locus_tag="VN496_04510"
     CDS             16760..17059
                     /locus_tag="VN496_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HXF16241.1"
                     /translation="MAIEAIGPTALAIPLSRAAVGETVRICKVLTGVHEGGGDMGLRL
                     LELGFVEGEQLRIIAHGFPGREPIAVRIGNTTFALRRFEADHVWVVPSSLSTAQS"
     gene            17056..>17497
                     /locus_tag="VN496_04515"
     CDS             17056..>17497
                     /locus_tag="VN496_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HXF16242.1"
                     /translation="MTAVQSSLRVALLGNPNCGKTALFNLLTGSRQKVANYAGVTVER
                     KEGLFETTSGRRIRVLDLPGAYSLNALSLDETITRDVVLGTKQGEDRPDLLVCVTDAT
                     NLRLNLRLVVEAKRLGLPMVLALNMSDVAKHRGVKIDVRALEREL"
BASE COUNT         3808 a         4752 c         4980 g         3957 t
ORIGIN      
        1 ctcattgccg gcatgcagct tggcacggtc gacatggctt atctcggtgt cggcaatgcc
       61 gggcagctaa aagggtgtgg ggcgctcaac gtttcctatc tgccctatct gttcaagagc
      121 aagcgcgctg cggaagatgg ccttaacggg ccgatattcc aggaacttta cgagcaatgc
      181 gcacagtccg ccggtgtgcg aatttttgcc gtgggcggcg accgtagtcc gcgcgctatc
      241 gttacgacaa gggggcccat tctcaagccc gaggacctca atgggatgcg gctgcgcatt
      301 ccccctatcc cgatcttccg cgagaccttc gaggcggttg gagtaaaagt agtaccactt
      361 gggcttaatg aagtctacat ggcattgtcg cgcggtcagg ttgatggcca ggataatggt
      421 tttgacctca cattgtcttt ccgcttccat gaagtttcaa aatactggtc ggcgaccgac
      481 cattgttatg acatgggcgc tttctacgtt tccgagcgca aatggaaaac ttttacgccc
      541 gagcagaaag ccttattcaa acggggggct gaagaaggtg gcaagctggc cactcagctt
      601 ggcgaagaat ttgacaaacg cggactcgag gaacttaaaa aagccggagt gaattatgtt
      661 gtgccggaca aggcagcatt cgcgagagcc tgggccaacg tatacaagag ctatgaaggc
      721 aaagtctggc ccgatggctt ggtgcagcgg atcaaatcag cccagaaata gcagtggtgg
      781 attttctgga caaggcgtta cgcctggccg aacgcgctct agaatggctc atttctatcc
      841 tggtcgccgt gctgctcgtt atcgtcgcgt caacgtttat cgaccggcat ttcgtgacat
      901 tgcccatggc ggcgccggac tcatacgcgc gggtcattct tgtctggctt acattcatcg
      961 gttttgcact ggcagtcaaa aacggactta acatccgcgt tgatctgatc gattcccgcc
     1021 ttccggccaa actgcggcgt ctgttcgaat acgttttcga tctgacgatg ctgggcttga
     1081 cgataatgct tggcctgaac ggatggcggt tagttatcat cggcctcgat cagcaaaggc
     1141 tcggcacaat gatgtcagaa gcctggccat ctggcgcctt cttcttgacg tgcattattc
     1201 ttgtcttgtt cctgctgctg cgcatcgcgc ttcgatttta cggtcgggaa ctgccgcagc
     1261 accacctgcc gctcgaggtc gaataagccg tgctgctgct gctgttcatc gcgttcatgc
     1321 tcgcgctaat cattgggttc gatgtcggtt tctcgatgat tctttccgca tggctgggcg
     1381 tgctgtaccg gggcgatgca gtggacccgg ccatgatgcc gctatcgatg attgcaggag
     1441 tggacaccta tgcactggtt caagtgccgc tgttcatcct tgccggcgaa ttgatgaatc
     1501 gcggtggcct gacgatgcgc ctgattgact gggctcaagc catggtcggc cgggtcaaag
     1561 gcagtttggg ccatgtttca atcctgacga atctagtgat ggccggcgta tcgggttccg
     1621 ccgtcgctga tgcagtcgca accggcaagc ccttgatccc cgcgatgcaa agagaaaagt
     1681 atggcgaagg cttcgctggt gccgtcatcg cggctggagc attgctcggt ccgattatcc
     1741 ctccgtcgat tccgatggtg gtatatgcac agctttccag ccaatcggtc gtcaaattat
     1801 tcatggcggg catcgttcca ggcctgttgc tcgcagcggg tttcatggcc gtgtgctcag
     1861 tagtagcgcg tgtgcgcaat tacccgagcc ggcagcccgt gccttggcgc ggcaaagtca
     1921 aggctaccgg tcgggcgggc tgggcgttgc tgatgcccgt aatcatcatc ggcggaattc
     1981 gcttcggtct agtcactgat acggaagcgg ctgccgtggc ggtgatctat tcgcttatcg
     2041 tgagcttgtt tgtctaccgt ggcattggac tcggggaatt gcgcagattg ctgttcgaat
     2101 cgggacgctc aaccgctgtc attctgttct tgcttgctgc ggcgggtccg ttcagctggc
     2161 tggtcgcgga gagccaggtc agccaaaccg tggtccagac tattcaaggc tggtcggcca
     2221 atcccatcat cgtgctactg ataatcaatg cctttttgct gttagttggg tgcatcatcg
     2281 agccgttgcc ggccatgatt atttttctgc cagtgcttat tccgctcggg gcgcaattgg
     2341 gcatcgatcc gatccagttt ggagcggttg tcgtccttaa tttaatgatc gggctgatgc
     2401 atccgccgat cggtctgctg ttattcgtcg tttcaagtgt gggcaagatt cggataggcc
     2461 ctgtgatgtg ggaaatactg ccttttctcg cctgggccat cgttgtcctg atcctgacta
     2521 tcgtctaccc acctttaacc atctggctgc cggcgcaaat caagtgactt tacattttgg
     2581 tgcgcgagta gatcaggaag cccgccagat ctttggctta taatcagaag catcgacgga
     2641 agcttgtcac gtcttccatc cagtttctcg atgcagcttt ttgcttttgg catgaaccac
     2701 catacggccc cgctcgcggt tcgcgaacag gtgacgtttc atgccgaaaa tatagacgct
     2761 gcactgcgcg acctcgtcac gcacgagtca gtgcgcgaag ccgccataat ttccacctgc
     2821 aaccgcaccg aaatctactg cagcactcct gaaccagttc gcgcgatcga ctggctagcg
     2881 cgcttccatc atttgaaggc tcccgctctg gaaccgttcc tatatacgct gccccgtgag
     2941 cgtgcagtaa atcatgcttt tagggttgcc agtgggcttg attcaatggt gctaggggag
     3001 ccgcagattc tcggccaaat gaaagaggcg gttaaatcag cggagcacgc tggaacgctt
     3061 ggtacggtgt tgcacaggct gttccagtca actttttccg ttgcgaagga agtacggacg
     3121 cagactgaca tcggtgcccg ttcagtgtcg cttgctgcgg cggctgtcaa agtggcggaa
     3181 cgaatctacc caagcatagc ggggcagaag atcatgtttg tgggcgcagg cgaaatgatc
     3241 gaattatgcg cgacccattt cgccgcgaga aagccccgcg aactgacgtt tgcaaatcgt
     3301 tccgttgacc gcggcgtcac actcggcagc cgatttagcg gcaatatcat tacactcaac
     3361 gatctgccgg aacagctggc attgcatgac atcgtggtta cctgcactgc aagcacgctt
     3421 ccgatcctgg gcaaaggaat gatcgagcgt gctatcaagg cgcgccgcca tcggccgata
     3481 ctgatgattg atttagccgt gccacgcgat gtggaggcgg aagttgccga gctggacgat
     3541 gtgtttctct acgacctgga cgacttgggg aaaatcgtgc aagagggtct cgatatccgt
     3601 caggctgcgg tggcgcaggc agaggccatc atcaatacca atgtgcatgc tttcatgcaa
     3661 tggctggata accgggaggt cgttccaacc atacgtgcgc tgcgcgatca ggcagagcaa
     3721 acgcgccgtc atgagctcga gcgcgcttcc cgtatgctgg cgaagggtga cgagcctgcg
     3781 aaagtgctag agcagctgtc gcattccctg accaacaagt tcctgcatgg acctacccag
     3841 gcactcaacg gcgttgaagc caatgaacgt gaggcattga tcgccgcatt gacgcgcttg
     3901 cacggggtaa agccacctga atgaaaccgt ccatcgcctc caagctggcg cagctgtctg
     3961 ccaggttgga ggaactcaat gctttgttgt ccagtgagga cgtcaccaag gacctggaaa
     4021 attatcgcaa gctgacacgc gagcatgctg aaatttcccc ggctgtcgag ctctacaatg
     4081 cctacgtaaa gagcgcgcag gacatcgcgg ctgcgcagga catggccaac gatccagaga
     4141 tgcgcgaact ggcagaaacg gaaataagcg agggcaagaa acgcctgcta gaacttgagg
     4201 cggaactcca gaagcggctt ctacccaaag accccagcga cgaacgcaac atttttcttg
     4261 agatccgtgc tggtaccggg ggcgatgaat cggccttgtt tgcaggggat ctgttccgta
     4321 tgtattttcg ctatgccgag cgtctggggt ggaaggtgga actcatatcg caaagcctgg
     4381 gcgaaatggg cggatacaag gaagtgattg ccaaaataat tggcttcggc gcttactccc
     4441 aactgaagtt tgaatcaggc ggacatcgcg tacagcgcgt tccagcgacc gagacccaag
     4501 gacgcatcca taccagcgct tgtaccgtcg ctgtcatgcc tgaagccgat gcaattgaag
     4561 acgtggtgct gaatccagcg gaattgcgcg tcgatacctt ccgtgcttcc ggagcgggcg
     4621 gtcagcacat caacaaaaca gactccgccg tacgtatcac gcacctcccc accggcatcg
     4681 tcgtcgaatg ccaggacgat cggtcacaac acaagaacaa ggcacaagca atggccgttc
     4741 ttgcggctcg catcaaggac aagcaggtcc gtgagcagca ggcgcaacaa gccgcaaccc
     4801 gcaaatcgct gatcggcagt ggggaccgct cggagcgcat acgcacctac aattttcccc
     4861 aaggcagggt aacggatcac cgcattaacc tcacgcttta caagatcgac cagatcatgg
     4921 acggcgcgat ggatgatcta attcatgctc tgcaggccga gcaccaaacc gagttgctgg
     4981 ccgcgatggc agaggaaaca ttttccctgc aagcgtgact ccaactgtaa gcgtgcttct
     5041 ggaagaagcg tcacaccagc ttgcctgcgc tgagcttgta gaggcacggc gggaagcgca
     5101 agtgttgctg ggtcacgcgc tgggcaaatc gcgcgcatgg ctttcggccc atagcgagga
     5161 tcaggtcgat ccgtcagtcg ttgcgaggtt ccgccaactc gtgcagcgcc gcctgtctgg
     5221 cgaaccggtt gcttatatag tcggtagaaa agagttttat ggtctggaat tccgtgttac
     5281 gcctgacgtt ttgatcccgc gtgcggacac ggaaacgctg gtagacgccg cgcttgcaag
     5341 aatgtcagcc gcgggctgcg acgtgctcga cctgggtacc gggagcggct gcatagcgat
     5401 cgccattgcg catgagcggc cagctgcacg cgtgatggcg gtggacgttt cttccgcggc
     5461 attgtccatt gctcgcgaaa atgcgttggc taatgcagtg gacgtgcaat tcctgcaagc
     5521 cagctggttt gaagcgctcg atgggcggcg attcgacatc attgtttcca acccccctta
     5581 cgtagcaact gaagatcccc atctgcagca aggcgattta cgtttcgaac ccgcaatcgc
     5641 actggcgggg ggcaatgacg gcttggccga cattcgcctg atcgtagcaa aagcgccaag
     5701 ttatctgcgc gaccaaggat ggttattgtt cgagcatggg cacacccagg cgtgcgatag
     5761 cagaaacctt ttggccaacg cgggtttcca ggacataacg cattcccggg acattgctgg
     5821 tttggttcgc gttgccggtg gtcgcctatt gagttgaaat ctccgaatcg ttaacatcaa
     5881 tagtatcggg agcagtgtcc cagcagcttt gggcttccca acatttttag aggtattact
     5941 catgaatgct caagacaaga tcaagcagca ggtaactggc aatccgatcg tgctttacat
     6001 gaaaggcacg ccagatgcgc cgatgtgcgg gtttagcgcc gctgcagtgc agatactgga
     6061 agcatgcgga gccgagaacg tggctactgt agatgtgctg agcgaccagg atgtccgcca
     6121 gggaatcaag gaatactcaa actggccaac cattccgcaa ctctatgtga acggcgaatt
     6181 cgtcggcggc gcagacatca tgcgcgagat gtatcagtct ggagaattgc agaagctgtt
     6241 cggcaaataa tttctgaaac agaatcgggc tggctgcgtg ccagcctttt ttttgtacgc
     6301 gtgccggcac cgcgcacaaa tcgacatcct ccttgttccg cctccctatt gccgcgtgaa
     6361 gttcagcgct atcatggccg ggggcaatgc aaaataaata gcgcgacttt catttcacac
     6421 gctgatccgc tcgagggaga aacatcatga tggaactagt gcgggtcgcc ttgcgccgcc
     6481 cgtacaccgt cgcggtcctt tcattgctcg tgcttttact gggcggcctg gccgttacgc
     6541 ggatggtggt cgattttttt ccgaagatcg atattccagt cgtgtttgtg ggctggaact
     6601 atcccggcct gtctgccgag gaaatggagc gtcgtgtcgt tattattagc gagcgcgcat
     6661 actccactac tgtcaacggc attgaccgca tcgagtcaaa ctgcatcccc ggcatcgggc
     6721 tggtaaaggt gtttttccac caaggcaccg acctcggcgc atccattgcg cagatcaatg
     6781 ccatcaacgg aaccattctg cgcatcttgc cgccggggat cacgcctcca gtcatcattc
     6841 agtatgacgc cgctaccgtg ccgatcgtgc aaatggtgct gtcgagcaag acaattccgg
     6901 aagggcggat tttcgaccat gcgttcaatt ttgtacgggt gaagctcttc accatacccg
     6961 gcctgaccgt acctgcgcca ttcggcgggc ggttgcgcca gatcatggtc gacctgaatc
     7021 cgcagttaat gactgcaaaa ggcatttcgc caaacgacgt ggtcaatgca ctgcagagtt
     7081 cgaatgttct ggttcccgcc ggaacagcgc gcatcggctc catggaatac aacgtgatga
     7141 tgaactccag tccgccaacc gtggaccagt tcaacctgat gcccctgaga atttccaatg
     7201 gcgttccggt catgctgggt gatgtggcga aggtggcgga tggttttgcc gtgcagaaca
     7261 acatcgtgca cgttgatggt cgccgtgcga cttatctgac gattctgaag cattccgagg
     7321 cgtcatcgct tgcagtagtg gaagcgacac gagcactgct tcccgagata cagaaagcgg
     7381 cgcctgaagg aatggaaatc aaactggatt tcgaccagtc ggtttacgtg cgcgctgcaa
     7441 tcaaaaatgt tgcgaccgaa gcagtcatcg catcacttct ggtgtccctg atggtgctgc
     7501 tgttcctggg ctcctggcgc aataccatcg tggtgattac ctccatcccc tgtgccatgg
     7561 cggcaggcat actaggtctg tactttaccg gcaataccat caatctgatg acattggccg
     7621 gactttcact ggctaccggt atcctggttg acgatgcgac cgtggctgtg gaaaacattc
     7681 atcgtcatcg cgcgatggga caacccctga cggtcgccat tctggacgga tcacgcgaag
     7741 tggcgttgcc acgcaccatg gccacgctgg ctatctgcat cgtatttatt ccggtggctt
     7801 tcctttacgg tgtctcgaaa tttcttttca tcccgcttgc gctgtcggtg gtgttcgcac
     7861 tgctcgcttc atacattctt tcttttacgc tggttccgat gctatcgaga cagctgctgg
     7921 ccggtgagca tcatgaggat gttgacgaca gaagtttcgg agcgcagctg aatcgcgtgc
     7981 gtgaaggcac gctggaacgc ctgcaaaaga cctacggcgc gatcctgacg atcatcatgc
     8041 accaccgtcc atttgtgctg gtttgcctgc tgctgatggg gggagtcagc gtctttctgg
     8101 gacgcgtagt cggcaccgat ttctttccca cggctgatgt aggcattctt aaacttcatt
     8161 tccgcgcgcc aatgggcatg cgaatcgaag ataccgagaa actcgtgctc gatgtacagg
     8221 aggaaatccg cagaatcatt cccgccgggg agatttcaac catcaacgac atgctcggag
     8281 taccgctatt tttcaatctt gccttcgtac cgaccgacaa tattagcggc atggatgcgg
     8341 acatcctgat ttcattgaag catccccacc agccttctgc ctattggatg cgagaactca
     8401 gggccaagct ggcgccgaaa tttcccgggg cgaccatgta ttttcagaat gccgatattg
     8461 tcacgcaagt gctgaacttc ggccttccgg caccgatcga cgtgcaaatc caggacgcaa
     8521 atttcgatcg cgcccaaggg tatgctgcgc gcgtgcgcaa aatcatcgag gaaaccccgg
     8581 gtgcagtcga tgtgcgacag atgcaagttt tgaactatcc ctccttgcgc gtggaagtcg
     8641 atcgcgtgcg cgcagcgcgg ctggggctta cgcagcgtga tgttgccagc aacgtactga
     8701 caacgctgtc gtccagtgcc atcgtgaatc cttcgtattt cctgaacccg aacggtgtga
     8761 actataccgt cgccgtgcag acgccaatcg agcgcatgag ttctgtcgat gatctgatgg
     8821 ctactccgat aaccacgggt tcaactccga tcgccgatac gacggccgtg gcacgtcttg
     8881 atgccaggcc gggtgcgcca gtgacccgcc tgtcgaatat cgcgtccgtc aagccggaga
     8941 caagctatca atcggtgtcc cattacaccg tgcagcgcgt gctcgatatt gcggccaatg
     9001 tcgacgggcg cgatcttgga ggtgttgtct cggacatcaa gcgcaagatc gatgcaatga
     9061 ccgcggaaaa ggatttcccg ccgaccgcaa gaatcaatat ccgggggcag tatcaggtga
     9121 tgaattcatc gttcgaaagc cttatcggcg gtctggtgct ggcggttgtg ctggtgtatg
     9181 ccctgctcgt ggtgctgttt cagtcatgga tcgatccttt catcatcatg atggccgttc
     9241 cgggcgcgct catcgggatt ctatggatgc tggctttgag cggtacgaca atcaatgtca
     9301 tgtcgctgat gggcgccatc atggcggttg ggattggcgt atccaattcg atcctcatcg
     9361 tcagttttgc caacgatctg cgcgtggaaa ggaatcttgg cgtgatagag gcagcagtag
     9421 aagcgggagt gacgagatta cgcccggtgc tgatgacggc cctggccatg attattggca
     9481 tggtcccaat ggcgcttgga ttgggagagg ccggcgagca aaacgcgcct ttgggtcgcg
     9541 cggtaattgg tgggctggcc atggcgacca tcgcaacgct tttcgtggtg cccgttatct
     9601 atactttcgt aagaaagaac atgccggtac tgcatggact cgatgcccgg ttcgaagctg
     9661 agtcgcaggg acatcacgcc tgaggagcaa tgagcatggc cgcaatgcag cgacgacggg
     9721 attcgtttct tgttttttat atcatcgctt ttgtggtggt cgcagccgca ggcgcatcgg
     9781 tctggtggtt ctggcagacc aaacagagtc atcttgcaga tgaagccaag gcacgcacag
     9841 cacaagtcga cgccggtccg acagtgctgg tgggtcagtc cgtgcgtgga tcgcctacgc
     9901 gcaagatcag tctgaccggc gagttgcgtc cgtggaaggc gactaccttg tattccaaag
     9961 tgggtggcta tctatccaag attactgtgg atgtgggcga tcatgtgcgc gcaaaccagc
    10021 tcattgcaga ggtccaggca cctgaactcg aatcggaata caaaagcacg atctcggaga
    10081 tgcagaatcg ggagcgaatt gcgcaacgta cgcgcgacct cgccgcaaag ggttttttct
    10141 cgcaacaggc gctcgataac gccgataccg acgtacggac gtcacgcggt cgcgtggata
    10201 cattgcgttc ccaaatgggc taccgaagct tgtatgcgcc ttatgccgga gttgtgaccg
    10261 gtcgctacgt tgacgccggc gcgttgatca ccaatgccgc taacaaccag acgtcatcac
    10321 agccagtcgt aacgatcgca gacccttcga agctgcgcgt tagcgtctat gtcgaacaag
    10381 tcgacgcaat ctatgtccgt cccggcatcg acgcggaaat cgttgatacg gcaaatccga
    10441 cccagaaaat taaagccaaa gtcagtcgcg ttgccggtga actcgacccc agaactcgca
    10501 ccctgctggc cgaagtggat ttcgacaatc gtaccgggac attcattccg ggttcgttcg
    10561 tgaacgtatc gctcattgtg ccgcaggcaa gctacatcga agtgcctgcg gctgctctgg
    10621 tggtacggga ccgcaagaat tttgttgcgg tacttgaaag cggcaatcac atcaagctca
    10681 cgccgatcga agttgccggc acggatggtt cggcagtaag aatcatcaat ggcctggggg
    10741 agggaacgag agtggtcctt agccttgcga atactgtgcc ggacggcgca aaggtaaatc
    10801 ctgcggccac gccgatggct tctgcacccc cggcgcctgc ggcagccgca actccagcgg
    10861 ccaaggccgt gacggcaaat cccggcgcgc ctgttcccgc tgtgccccag acaccgtctg
    10921 ccatcgcgac aacgcccaaa accgagggcg taaattcgca gcggcccaag taacctgtcc
    10981 tataattcac cacgcgtgcc ggatcgcgca ggtaattaca gcagatcatt cagaaggcgt
    11041 tatcgatgat gaactatcgc ggcattcatg atatgggtgg attgcccgcc ggcagggtcc
    11101 aagcgaccga gcatgactat gctttgtggg aaaagcgcgt cgatgccctg ttggtcctgc
    11161 tctccggcaa aggatacgtt actgtagatg aactgcgtcg gaacatcgaa acgcttggtg
    11221 ccgaggccta tgacaaaatg agttactacg agcgttggat ctatgcgatt acacagacgc
    11281 tgatccagcg cggcaccgtt accatagaag agctcggtgc ggaaattgcg caagcgcaag
    11341 cccatggcga acgctgattc gattgacccg ctcttcaggc ccggtgacgg cgtcaccgtc
    11401 cgagcaatct atccggtcgg acatgttcgc acaccgtact atattcgcgg caagcgcggg
    11461 gtcgttgaac gtctctgcgg cagttatggc aatcccgagg aacttgctta tgcaaggagc
    11521 gggcttccgc gtcaaccttt gtaccgtgtg cggttcttgc aacgagacgt ctgggcagac
    11581 tatgccggcg ctgcgcacga taccgtcgac attgaaattt accagcactg gctggaacgt
    11641 taatggcaca cgaccatcat cattcgcacg acgagacacc gcacaagcat cccccgctgc
    11701 gtcccgacca ggacgacacg attacttatt ggcgcgcgat ggaaatcgcg gtgcgcgggc
    11761 tgcttataaa aaaaggcatc gtctcggccg atgaagtacg ccgaacagtt gagcagatgg
    11821 acaatcgcac cccgctgcaa ggcgcaaaga ttatcgcgaa agcgtgggtc gatgctgatt
    11881 tccgcaagtt gcttctgtct gacggcaatg ccgcctgcaa ggcgctcggg gtggaagaag
    11941 gattctacaa gctggtggtc gtagaaaata cggagaaggt acacaacgtc atcgtttgca
    12001 ccctgtgctc gtgttatccg cgctggctgc tcgggctacc acccgattgg tacaaaagcc
    12061 ggaattaccg ttcccgcgtg atcagtgaac cgcgtgaggt attgagggag tttggtacgc
    12121 aaatcgccaa cgatacggtg gtacgggtgc acgactcgac tgctgacatg cgctatctcg
    12181 tccttccgaa gcggcctgcc ggaaccgatg gatggtccga ggataaactc gctgcactcg
    12241 tgacgcgcga tgccatgatt ggtgtcgcga atgctcaaca tccttgaacc tcgctcacca
    12301 atctgcattc gccagcttgt ttcaatggcc agagccgaag gcgaattttc ctgcggcatg
    12361 gcctggtctg cggaattgcg tgccgggtgt tgaacggtga aggtcaacgg aaaggaatga
    12421 aatcgccgaa tcttgacgcg atcaagagca ttgcttttgg gcagccgagg cgattttttc
    12481 agccaccagc caaaggtgaa gtcctggcca atggagagcg gcgctatttt ataggcgatg
    12541 cgattggcca gggcgggttc gggatggttt atgactgtat cgacgactgg gggaacccgc
    12601 tggtcgcgaa aattctcatt ccacacgagc agtcctaccg cgaagtgcgc cgagtctggc
    12661 aagaggaact gcgcaagctg cttgtgatgc gccatcccaa tatcacgttc gtttacgatg
    12721 cgttcgagta caaagacacg ttttatctga tcattgagcg ctgcagtttt ccgttatcgc
    12781 gcttgatcac gcggcttgga ctggacgccg accgttggat cccgtacgtg gcgcgcgatg
    12841 tgttgcaggc actcgattac attcatgccg caggatacgt ccacaaagac ctgcatccgg
    12901 gcaatgtctt cgtcacggag cagaggcgca agcgcaggct gatcaaagag caggtctggt
    12961 cgttcaaagt gggtgacctt ggcctgaccc gccttgaatc ggacattcgt gtgttcaaga
    13021 cgctgcttgc cgaatggatg cgcccgccag aagcgtttga gccggagaaa tttggcgcgg
    13081 tcggccatca ggtggatatt tatcagactg ggttgttgtt gttggccctg atgctgaagg
    13141 aaatcccgta tttcacccgc gacgaaatcc tgggcggtat tccccaggcg ctggccgcca
    13201 agcacgagtc gaagttcggt cccgtcgttg caaaggcgct gcgccgtcac gtggaatggc
    13261 gaacacagac ggccctggaa ttctggcgcg agttgtctgg cgtagcgggt gcaagtccac
    13321 cttgagcttg atgcgcgagt accgctttgc accatcaagc agaatcagga acgcgcggct
    13381 cgaatcaata gcgttttttg ttggttagcg cgactaacgc ggcaacgatc aacagcacaa
    13441 agcaaatcag cgaccattca gcgattgaca ggcccaggaa gcgccacagc actttagtgc
    13501 agtctccggt gcctcgaaaa atcatcggca gcgcttctgt gagtggaaac gactccagca
    13561 tgaatccgac gtccgcgcca cagtcgaata tttttggtgg attgtgctcg aggtagacat
    13621 gcctgattgc cacgccggcg cccgtcgcgg cgaacagcag tattaggcca ctgtaaaccc
    13681 gttgtccgat taaagcaggc ccatgcaggg ctgctaccaa ggcaatcacg ccaaccacaa
    13741 tgaaggcgta gcgctgcaaa atacacatcg ggcagggttc cagcccgaca gagtgttgca
    13801 ggtatagcgc gaacgcaatc agtccggcgc aaaccagaaa catcacaagg tggatcgaac
    13861 gaggcgttgc tttcatcaga tcaaggccaa tgcgtggtcg aggtccgcga tcaaatcttc
    13921 aatatcttct atgccaactg ataatcgaac aagattgtcg gtaataccaa gccgtgcgcg
    13981 aaggtctgac ggcaatgaac cgtggctcat ggtgaccggg tggcctatca ggctctccac
    14041 gccgcccaga ctttccgcga gggtgaatat tttgcaggct tccagcgcgc ggcgcactcc
    14101 attcatatcc gtcttcagat aaatcgaaat catcgcacca aaaccattca tctgttttgc
    14161 cgccaacaag tgttgtgcat gatttggcag ccccggataa atcacttttt ccagtttcgg
    14221 atgtgacttc aaccaagtgg ccaaatgcat ggcattcccg caatgctgtc tcatgcgaag
    14281 cggtaaggtt ttcagaccgc gtaatgccag gaagctggag aagggatcga ggatcgatcc
    14341 gatcgcgttc tgcatgtatt cgagcttttc ggcaatgtcg ccgcgttcct tcacaatgac
    14401 cgctccgcca accatgtccg aatgaccgct gatgtacttg gtcaccgagt gcagcacaat
    14461 gtcgatgccc aggtcgagtg ggcgctggat gaacggcgaa gcaaaagtgt tgtcaacgac
    14521 cgtcaggatg ccacgcgctt ggccaatcct ggcgacctct gcgagatcgg tgattttgag
    14581 gagcggattg gaaggcgtct ccacccagat catcttcgtg tcaggctgga tcgcttgctg
    14641 cagcgaaccc gtcacgttcg gatcggagta gcttgttcgc agacccatgg aacgttttct
    14701 gacgcgctcg aacaggcgcc acgaaccacc gtacaaatcg tctgccgcaa cgatgtggct
    14761 gcccgaatcc agcaattcca gcaccgtact acttgccgca agtcccgatg caaaagcgaa
    14821 agcacgcgtg ccattttcaa ggtccgctat gcaacgctcg aatgcatgcc tggttgggtt
    14881 ttgcgaccgg gcgtactcat agcccgtatg cacgccgggt gattgctgct tgaacgttga
    14941 ggtggcgaat atgggagtca tcaccgcgcc agtagtccga tccggtggct gtccggcatg
    15001 gattgatcgg gtggcaaagc cctgcgtcga tggttcattt ttcatgacga gttaccgggt
    15061 gagcgcatgc aatcagcatg catccagatt tggactgttt tagcactgtc ctggttgtgg
    15121 ctatggcgcg cggtgacgca gatggttcag cacgtccata cgggtgacga gaccatagaa
    15181 tttcccatct tctataacga gggctacatg gcctttgttg aacgtttcca gcagacgcgg
    15241 aatgggagtt tttagcccaa ccgtttcaag tctttgagtc atggcggaac gcacggggtc
    15301 cttgaagtgc tcgggatcct cgcgtgaagc cgtgagcaaa tcccactcgt cgatcagccc
    15361 gacgactttg ccgtcctcca gcaccggcaa ttgtgaaata tcgtacagtt tcatgcgagc
    15421 gtacgcagtc atgagcttat catccggccc aatggtaacc gtcgcctttt catcatggcg
    15481 gcgggtgatg aggtcgcgaa gatcgccaaa gtgttcgcgc ttgatgaagc cctgatccat
    15541 catccaatag tcattgaata ccttcgaaag atacttattg ccgctatcgc agacgaacgt
    15601 gactacgcgc ttggaagtcg tctgctcacg gcaatagcgt agcgctgccg caagcaaggt
    15661 gcctgtcgat gaacccgcaa ggatagcttc tttggcaagc agttcacgaa ccgtggtggc
    15721 cgattcttca tcactgatgg aatatgcttt cttgacgcgt gagaaatctg caatcggcgg
    15781 aatgaaatct tcgccaatgc cttcgacaag ccaactaccc gcttcaccgt atgtgccggt
    15841 acgtacaaaa tccgcaagca ctgatcccac cggatcagca agcactagtt cagtccgagg
    15901 cgatactttt tggaagtaac gagacagtcc tgttatcgtg ccggccgatc ccactccaca
    15961 cacaatcgca tcgacatcct gatgcatctg acgccatatt tcgggaccgg ttgtcgtctc
    16021 gtgtgcaagt ggattggccg ggttgccgaa ctgatttacg aagaatgcac cggtctcatc
    16081 cgcgatcgtt tttgccatgt cctggtaata ctcgggatgg cccttaccga cgtccgagcg
    16141 cgtcaacacg acttccgcgc ccatagcttt gaggtggaaa attttttcgc ggctcatttt
    16201 gtcaggaacc accaaaatca gcttgtaacc cttcagagtc gcgattagtg caaggccgag
    16261 tcccgtattg ccagcggtgg cctcgataat cgtgccaccc ggtttaagcc gtccgtctgc
    16321 ttccgccgcc tcgatcatcg aacgaccgat gcgatctttg atggaacccc ccggattttg
    16381 actttccagt ttcaaaaaaa tgcggcaccg accggcatcc atgccggtta tctcgacaag
    16441 aggtgtattt ccgatcagat caagcaaccg gttggtagcc aatcagaact tccttttagt
    16501 tgcccaagca tcgcacgggt tgtgtgggac cgcgtgtgtc agggaccgtt cctcgcgccg
    16561 caattaccga agaagcatcc acttgtaccc gtaaggcgag tttaactcgc tgaaccgcgg
    16621 ataagtgcag ccggatctgc acccggaatc ccaggcgcag gcccgagtgg tgttctgatc
    16681 cgggatgcca aaatcctcaa aagagcccat aataataata attcccatta acttaatcgg
    16741 tcccgggatg ccgttagaca tggcaataga agcaatcggc cccacagcct tggcgattcc
    16801 cttgagccga gcggctgttg gcgaaactgt ccgcatctgc aaggtgctca cgggggtgca
    16861 cgaaggtggc ggggatatgg ggttgcgctt gctggaactt ggattcgtgg aaggcgaaca
    16921 gctgcgcatt atcgcccacg gatttccggg tcgcgagccc attgccgtcc gcatcggcaa
    16981 tacaacattc gcattgcggc gatttgaagc cgatcacgtc tgggtagtgc cgtcgtcact
    17041 atccacagcc caatcatgac agcagttcaa tcttcgttgc gtgtcgcgtt gcttggcaat
    17101 cccaactgcg gaaagaccgc gctcttcaat ttgctgacgg gcagccggca aaaagtggcc
    17161 aattatgcgg gggtcactgt tgaacgcaag gagggattgt tcgaaacgac aagcggccga
    17221 aggatacgtg tccttgacct gccgggcgcc tacagtctga atgcgctaag tctcgacgag
    17281 accatcactc gggatgtagt tctcggcaca aagcaaggag aggaccggcc cgatttactg
    17341 gtctgtgtaa cggatgccac aaatcttcgt ttgaacttgc ggttggtggt tgaagccaag
    17401 cgcttgggtc tcccaatggt gttggcgctg aatatgagcg atgtcgccaa acatcgcggc
    17461 gtaaaaatcg atgtccgtgc actggagcga gagcttg
//
LOCUS       DATMZQ010000138         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4888712, whole genome shotgun sequence.
ACCESSION   DATMZQ010000138 DATMZQ010000000
VERSION     DATMZQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4888712"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VN496_04520"
     CDS             complement(<1..215)
                     /locus_tag="VN496_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16243.1"
                     /translation="MSAPTLQAATLLLDQRLSLQPITALPEGIRPQTEAEGYALQRVL
                     NGLLTVAGMGDQVGHKIGCTTPVMQKFL"
     gene            455..1930
                     /locus_tag="VN496_04525"
     CDS             455..1930
                     /locus_tag="VN496_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXF16244.1"
                     /translation="MKDQSHGLRRLTVLRGLLLSLVAASALAWAGVASAQSAAQKAVE
                     AAKKICNGKTITIVWEAGLQSLDPTQFSGPLWEKETGCKVKVVEVPTAEMFTKIMQEF
                     RAGTGAYDALNVIPAWMPDLAQAGALEVLDPYVDKYGFRDELKKIAPTYRDNQMTVNG
                     KIYGFPDDGDVFLFYYRKDIFARADLKKAFKDKYKYDLAPPKTWKQFDEIGSFLTDAL
                     KKDHIYGAAFFREPPYTMFMFQERFRVEGGKFFDANTMNATVNGPVGVKVFTEMRNEN
                     RFMPPGVEKFGFVENLATFLKGESAMTISWPPYGRFAAGYLANEKALSWVPKSTIAGK
                     VGYALPPGGHPELAAGFALSVASTSKQKEAAYLFIQWLNSEEISGKRVKLPYALRDPF
                     RDSHYTDPEYLKLWPDAKEYLSALHQGAQTGLLDLSLIQTDKYEEALRQGISKLWAGE
                     DPKAILDKVAADWDGITKQVGTDKQKAVYSAWASKSGAYPK"
     gene            1979..2941
                     /locus_tag="VN496_04530"
     CDS             1979..2941
                     /locus_tag="VN496_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXF16245.1"
                     /translation="MERGSAGLGAPFLSVLSLRDISGATHLMQSRGAFAERNFKYLLV
                     GPAIFVLLLIGIFPLVYLLMVSFQGITMTETSTDFVGARNYAQLFHDVRMWEALLHTV
                     IFTAIALPVELVLGLAMAQLFLERLPGRQIFVALLVLPVVVSPIVSGATWALLFDHRF
                     GPINQIIGWFTGDTTAILWSINPKLVYPAIIAAEIWQWTPFMFLLLLAALANVDKSQL
                     EAAAIDGAGWWRTFFRIVLPAIWPVMAIAILIRGLDLFRLFDIVWALTKGGPGTMTET
                     ISIFTYVKGFQQFETSYTAAVAFLVIILLSVVVMLALKRVELAR"
BASE COUNT          647 a          845 c          887 g          666 t
ORIGIN      
        1 agaaatttct gcatgacggg agttgtgcag ccgatcttgt gcccgacctg gtctcccatt
       61 ccggcaaccg tcagcagccc gttcagcacg cgctgaaggg cgtaaccctc tgcttctgtc
      121 tgtggcctga tgccctctgg aagcgccgtg atcggctgca gggataaccg ctggtcaagc
      181 aggagggtgg cggcctgcag ggtaggtgcg ctcatgcatc tccttcttca aaaatccaat
      241 tgcaccacaa attagagaca accggtcgca tgattaaact gcgtagacat gcatcggcgc
      301 cgggattgca tcccattgct gccgtgcggc atagattcgg tctggtgagt tgcttgacac
      361 tcaagcgggc ccttcaacat aatcgtcccg caccacagcg ttgcccgcca atgagcgagc
      421 aatcaaaaac ttataaatca tcggaggagg caatatgaaa gaccagtcac acggccttcg
      481 ccggctcact gtgctcagag gtttgctcct gtcgctcgtc gctgcctcgg ctctagcctg
      541 ggcaggagtc gcgagtgcgc agagtgcagc gcagaaggct gtcgaagctg ccaagaaaat
      601 ctgcaacggc aaaaccatca ccatcgtttg ggaagccggc ctgcagtcac tcgacccgac
      661 ccagttctcc ggcccgctct gggaaaagga aaccgggtgc aaggtcaaag tagtcgaagt
      721 cccgaccgct gagatgttca ccaagatcat gcaggaattc cgcgccggta cgggtgcgta
      781 cgatgccctg aacgtgattc cggcgtggat gccggacttg gcccaggcgg gcgcgctgga
      841 agtactcgac ccctatgtcg acaagtacgg ctttcgcgat gagctgaaaa aaatcgcacc
      901 gacataccgc gataaccaga tgacggtcaa tggcaagatc tatggctttc cggatgacgg
      961 ggacgtattc ctgttttatt accgcaagga catctttgcc cgagccgacc tgaagaaagc
     1021 gttcaaggac aaatataaat acgacctggc gcccccaaag acctggaaac agttcgacga
     1081 gatcggcagc ttcctgaccg acgcgttgaa gaaggaccac atctacggcg ccgcattttt
     1141 ccgtgagcca ccttatacga tgttcatgtt ccaggaacgt ttccgcgtgg aaggtggcaa
     1201 gttcttcgat gccaatacca tgaacgccac ggtgaacggg ccggtgggtg tgaaggtctt
     1261 cactgaaatg cgcaacgaaa atcgatttat gccgccgggc gtcgaaaaat tcggcttcgt
     1321 ggaaaacctc gcgactttcc tgaagggcga aagcgcgatg acgatttcgt ggccgcctta
     1381 cggacgtttt gcggccggct atctggcgaa cgagaaagca ctgtcctggg tgccgaagtc
     1441 aaccatcgcg ggcaaggtag gttatgcgct acccccgggc ggtcatccgg aactggccgc
     1501 gggttttgcg ctgtcggttg cctccacgag caagcagaaa gaagcggcct atctgttcat
     1561 ccagtggctg aacagcgaag agatcagcgg caagcgtgtg aaactgccct acgcgttgcg
     1621 cgatccgttc cgcgattcgc attacacgga tccggagtat ctgaagctgt ggcccgatgc
     1681 aaaggaatat ttgtccgcat tgcaccaggg cgcacagacc ggtttgctgg atttgtcgtt
     1741 gattcagacc gacaagtatg aagaagcgct gcggcagggc atcagcaagt tgtgggccgg
     1801 cgaagatccg aaggcgatcc tcgacaaggt cgccgcagac tgggacggca tcaccaaaca
     1861 ggtcgggacg gataagcaaa aggcggtcta tagcgcctgg gcttcaaaat ccggcgccta
     1921 cccgaagtaa ggagaatcgg cagggttcgc tccggcgatt aagcggcgag agcgaaacat
     1981 ggaacgcggg agcgccggac tcggcgctcc ttttttgtca gtactctctt tgcgggatat
     2041 ttcgggggca acacacttga tgcagagccg cggagccttc gcggaacgta acttcaaata
     2101 cctgttggtt gggccggcga ttttcgtgct gttgctgatc ggtatttttc cgctggttta
     2161 cctgctcatg gtgagctttc agggcattac catgaccgaa acatcgacag actttgtcgg
     2221 tgcacgcaat tacgcgcaac tttttcacga tgtgcgcatg tgggaggccc tgctgcacac
     2281 ggtcattttc actgccatcg cattgccggt ggaattggtc ctgggtttgg ccatggcgca
     2341 attgttcctg gaacggttgc cgggacgaca gattttcgtc gcgctgctgg tgctgccggt
     2401 agtcgtgtca ccgatcgttt ccggtgcaac ctgggcttta ctgttcgatc atcgtttcgg
     2461 accgatcaac cagatcatcg gctggttcac cggcgatacc acggccatcc tctggagcat
     2521 caacccgaag ctggtctatc ccgccatcat cgcggccgag atctggcaat ggacgccgtt
     2581 catgtttctc ttgctgctgg ccgccctggc caacgtagac aagtcgcaac ttgaagccgc
     2641 cgcaatcgat ggcgctggct ggtggcgcac attcttcagg atcgtgctgc ctgccatctg
     2701 gccggtcatg gcaatcgcca tacttattcg cgggctcgac ctgttccggt tgttcgacat
     2761 tgtgtgggct ctcaccaagg gcggccccgg cacgatgacg gaaacgattt ccatttttac
     2821 ttacgtcaag ggtttccagc agttcgagac gagttacacc gccgcagtcg cattcctggt
     2881 gatcatcctg ttgtccgtgg tcgtcatgct cgcgctgaag cgagtggagc tggcgagatg
     2941 agcagcttac gcagacgcac tgccaaaagg caggcgatcc ggtttgcggc tgcggtcgca
     3001 atcacgctga tcttcgtgtt cccgatttac tggctgttca tgatt
//
LOCUS       DATMZQ010000139         9015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4888799, whole genome shotgun sequence.
ACCESSION   DATMZQ010000139 DATMZQ010000000
VERSION     DATMZQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4888799"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /gene="lnt"
                     /locus_tag="VN496_04535"
     CDS             complement(<1..997)
                     /gene="lnt"
                     /locus_tag="VN496_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXF16246.1"
                     /translation="MQTDLLVAKRPVRLLVFLRLFVAPFFFGAIGVLGFAPFGLYVIP
                     VISLTALLLMWRHCISSRAAAIVGFSFGAGMFVVGVSWVYVSLHVYGGMVVPVAAFCT
                     LLFCLIQACYPALVGAVLVRSRGNFAAKALLGFPALWVFCEWLRSWVLSGFPWLSIGY
                     SQVPTSPLAGFAPILGAFGVSLVTAFIAGAIATAIESRFRAGNTPKAGKRLFVSLGVA
                     LCIAGTLIIAGALLKRHQWTQPLPGEPTTVTLLQGNIPQELKWRPERAIATLQAYLEL
                     VRSTDSKLILLPETALPMLNTDIPPDYLRALTDRTRLNGGDMLFGVPEQDASGRYY"
     gene            complement(987..1823)
                     /locus_tag="VN496_04540"
     CDS             complement(987..1823)
                     /locus_tag="VN496_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HXF16247.1"
                     /translation="MEEETHKPSLLERLGALLMREPEDREQLIELLQRSYERNLLDAD
                     ALAMIEGVMQVSEMQARDIMIPRAQMDVIDIGDAPEKFIPWVIETGHSRFPVIGENKD
                     DVIGILLAKDLLSYYAGEDFNVRDRLRPAVFIPEAKRLNVLLKDFRSNRNHIAIVVDE
                     YGGVAGLVTIEDVLEQIVGEIEDEFDFDETEANIILDRNGQFRVKAQTEIDDFNRTFG
                     TDFSDEDFDTVGGLVLAHLGRLPKRGEQFRIGDLNIKVVRADSRRLHALLVEKIRTGS
                     AD"
     gene            complement(1883..2347)
                     /gene="ybeY"
                     /locus_tag="VN496_04545"
     CDS             complement(1883..2347)
                     /gene="ybeY"
                     /locus_tag="VN496_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXF16248.1"
                     /translation="MAAKTVRSPPALLILSVQYGIAQTGLPSRQRMRSWAKPALRTDA
                     QVTIRLVGRREARKLNRAFRGKDYATNVLTFVYSDHEPLHGDIVLCAPVIAKEAREQR
                     KTIDAHFAHLVVHGVLHLQGYDHDNLKDAEVMETLESEIVVKLGYADPYKTG"
     gene            complement(2353..3306)
                     /locus_tag="VN496_04550"
     CDS             complement(2353..3306)
                     /locus_tag="VN496_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXF16249.1"
                     /translation="MNPLDISFTPVDNQRLANLCGALDENLKQIESAFDVAIARRGEQ
                     FSISGAPEPSRLAAEALQRFYQQATDSVSLEDVQLGLIEIAGSAAPTAPADALVLQTR
                     RRDLHGRTPRQVQYIRQIREHDITFGIGPAGTGKTYLAVACAVDAFERDAVKRIVLVR
                     PAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFEKVAKLFERAAIEIAPLAFMR
                     GRTLNHSFIILDEAQNTTPEQMKMFLTRIGFGSRAVVTGDVTQIDLAKSQKSGLVEAA
                     IILRDVRGIAFTQFLADDVVRHPLVQRIVQAYERHQVRQAD"
     gene            complement(3303..4637)
                     /gene="miaB"
                     /locus_tag="VN496_04555"
     CDS             complement(3303..4637)
                     /gene="miaB"
                     /locus_tag="VN496_04555"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXF16250.1"
                     /translation="MKKLYIRTYGCQMNEYDSDKMADVLNASEAFERTEDPAQADVIL
                     FNTCSVRENAQERVFHALGRLRPLKQARQDLIIGVGGCVASQEGQAIVERAPFVDVVF
                     GPQTLHRLPQLIDARRRSGRSQVDISFPEIEKFDALPAATHEGCTAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFDDVLTEIAGLADQGTKEITLLGQNVNAYRGKMNDGETADFA
                     LLLEYLAEMPGIKRLRYTTSHPREFSQRLVDSYRHLPQLVDHVHLPVQSGSDRILAAM
                     KRGYTSLEYKSIIRRLRTARPGISIASDFIVGFPGETEQDFAETMKLVEELHFDDSYS
                     FMYSPRPGTPAANFAEQVPLEIKQARLARLQAALDVSAKAISNAMVGQVERILVEGTS
                     RKNLHELYGRTSNNRVVNFPGSKRLIGKLMDVHITAALAHTLRGEVRVTEPV"
     gene            complement(4647..4970)
                     /locus_tag="VN496_04560"
     CDS             complement(4647..4970)
                     /locus_tag="VN496_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16251.1"
                     /translation="MKKLLHVLLASLLALLCVTQTCAARDFPQQARRGDMQAYRYPLM
                     KIGERVYRLAAGSYLYNQQNLIIMPASMNIQTAPVMYLLDTSGDLSRIWLLTREEAAQ
                     HPLVK"
     gene            complement(5113..5189)
                     /locus_tag="VN496_04565"
     tRNA            complement(5113..5189)
                     /locus_tag="VN496_04565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5153..5155),aa:Met,seq:cat)
     gene            5440..6597
                     /locus_tag="VN496_04570"
     CDS             5440..6597
                     /locus_tag="VN496_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HXF16252.1"
                     /translation="MIVDVAIVGGGPVGASVALALAPTSFSIALIEPRRPPLLPAAGF
                     DQRVYALNRRSRRFLERCGIWQHLLMDRIAPVREMRVFGDQASQLDFSAYRNGVPELA
                     AIVEEANLQQALQRALSSQSNLAVLAGVACLGATWDSEKATLSLSDGQQIAASLVIAA
                     DGADSALRSAAGISVRVHEYGQTGVVANFRAGQPHCDTAFQWFVEGSVLALLPLPANH
                     MSMVWSVPNAQAGALLSLTAEQLAEEVQRMSDNVIGLQPTGVAAGFALRRMQAARLIA
                     PRLALVGDTAHNVHPLAGQGLNLGFGDAESLAAVLATSGTHGDPGAYALLRRYERSRR
                     EDHLAMEVVTEGLQLLFASTVPGMKRLRNAGLRLVNRTAPLKRLLVKRALG"
     gene            6672..7391
                     /locus_tag="VN496_04575"
     CDS             6672..7391
                     /locus_tag="VN496_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HXF16253.1"
                     /translation="MFSLLALVTGVAFADEASIRAEFAKKYPQANIESISKTPYLGLY
                     EMLVDGEVIYTDPDFTYLIVGSLIETKTKVNLTDARQREIEEKKLKSMAFPFEQLPFD
                     LAIKKVKGDGSRKVAVFSDPDCPYCRRLEKDLEKISNATIYIFLFPIEQLHPKAPEMS
                     RAIWCAPDRVKAWDEYMLKGVVPKSPKCDTPVDKIVAYGQSKKINGTPTIFFADGKRV
                     PGAIPSERFEELLKASNPGKS"
     gene            7713..8138
                     /locus_tag="VN496_04580"
     CDS             7713..8138
                     /locus_tag="VN496_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXF16254.1"
                     /translation="MVASEPVSAAQPVAIPDVPVPEPQPSKDRDESSVIPDLVHYAAS
                     QLDVYPRALQTIAPAYPTGALETQTAGFVTLLVLIDEVGRVMGTTVVDATPDGVFEQA
                     AQQALAEAIFYPAQREGHSVRSRILVKVEFDPSAAAAMH"
     gene            complement(8143..>9015)
                     /locus_tag="VN496_04585"
     CDS             complement(8143..>9015)
                     /locus_tag="VN496_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HXF16255.1"
                     /translation="SSFAPWLVHNATGATEGLVTGYYEPLLYGSRHFGREFRFPIYAV
                     PPDLLAIDLAQTNPQLKGIRLRGRMQDKRIVPYYSRAEIESGLAPVRGLEIVWVDDPV
                     ELFFLHVQGSGRIKLPGGDILRVGFAEHNGHPYVSVGKLLVDRGELPLDKASMQGIKQ
                     WAAKNPGKVVQLLQQNPAYVFFRELPGSTAGPIGAQGVPLTPGRSIAVDPLTTPLGAP
                     VFLSTTAPLSMRPLNRLVIAQDTGTAIKGPIRADFFWGFGDDATQSAGRMRQTGTMWI
                     LYPRGMQPRELPPP"
BASE COUNT         1981 a         2546 c         2536 g         1952 t
ORIGIN      
        1 tgtaatagcg tccggaggca tcctgttccg gaacaccaaa cagcatgtcg ccgccgttca
       61 gtcttgtccg gtcggttaat gccctgagat agtcaggcgg tatgtcggta ttgagcatag
      121 gcagtgcagt ctcgggcaac aagatcaact tgctgtctgt cgagcgtact aactccagat
      181 aggcttgcag cgtggcgatt gcgcgttccg gccgccattt cagctcctgc ggaatatttc
      241 cttgcagcag cgtcacagtg gtcggctccc ccggcagcgg ttgggtccat tggtggcgtt
      301 tgagaagggc tcccgcgatg atcagcgtgc cagcaatgca cagagccact cccaaagaga
      361 cgaaaaggcg cttgccagct ttcggtgtgt taccagcgcg aaagcgggat tcaatagcgg
      421 tggcgatagc ccctgcgatg aatgcggtaa cgagggacac gccgaatgca cccaggatgg
      481 gagcaaatcc cgccagtggg ctggtcggaa cctgcgaata gcctatggag agccacggga
      541 agccggagag aacccagctg cgcaaccatt cgcaaaagac ccacagcgct ggaaagccca
      601 gcagtgcttt tgcggcaaaa ttcccgcggg accgcacaag gacggcgccg accagcgccg
      661 gataacaggc ttggataaga caaaacaaga gcgtgcagaa cgcagcaaca ggcacgacca
      721 ttccaccgta aacgtgcaag cttacgtaca cccagctcac gccgacgacg aacattcctg
      781 cgccaaaact aaatccgacg attgccgctg ccctggaact gatgcaatgc cgccacatca
      841 acagcaatgc agtcagggag ataaccggga tgacatagag cccgaaaggt gcgaatccga
      901 gcacccctat cgcgccaaag aagaaaggtg cgacaaacag gcgaagaaaa accagcaagc
      961 gaaccgggcg ctttgctaca agtaagtcag tctgcactcc cggtcctgat tttctcaacg
     1021 agcagcgcat gtaatctgcg gctgtcggcg cggacgacct tgatattcag gtcgccgatg
     1081 cggaactgct ctccgcgttt gggcagcctg ccgagatgag ccagcacaag gccgccgacg
     1141 gtgtcgaaat cctcgtcgct aaaatcggtg ccgaaggtcc tgttgaagtc atcaatctca
     1201 gtttgcgcct tgactctgaa ctggccattg cgatccagaa taatgttcgc ttctgtttca
     1261 tcgaaatcga actcgtcttc gatttcgccc acgatctgct cgagcacgtc ctcaatcgtt
     1321 accaggccgg cgacgccgcc gtattcgtcc acaacgatgg cgatgtgatt gcggttgctg
     1381 cggaaatcct taagtaatac gtttaaccgt ttggcttccg gaataaacac cgcgggtcgc
     1441 aaacggtcgc gtacattgaa atcctcgccc gcataatagc taagcagatc cttggcgagc
     1501 agaatgccga tgacatcgtc tttattctca ccgattaccg gaaaacggga atggccggtt
     1561 tcaatcaccc aggggatgaa cttctccgga gcgtctccta tgtcgataac atccatctgc
     1621 gcacggggaa tcatgatatc ccgggcctgc atttcggaaa cctgcatcac cccttcaatc
     1681 attgcgagcg catctgcgtc aagcaaatta cgctcgtagg aacgctggag cagctcgatg
     1741 agctgctccc tgtcctcggg ctcgcgcata agcagcgcgc ccagccgttc caacagtgaa
     1801 ggtttatgcg tttcttcttc catgtagcaa tggcgatcag tgagcgtaaa ggacaactgc
     1861 aaccacggca ttagtgcgcc gttcacccgg ttttgtaggg atcagcatac cccagcttta
     1921 cgactatttc cgactctagc gtttccatga cttctgcatc cttgagattg tcatgatcgt
     1981 agccctgcaa atgaagaacg ccatgtacca ccaggtgtgc aaagtgcgcg tcaatggttt
     2041 tgcgttgctc tcgggcttct ttcgcaatca ccggagcaca cagaacgata tcgccgtgca
     2101 gcggctcatg gtcggaatag acgaacgtca atacgttggt tgcgtaatcc ttgcctctaa
     2161 aggcgcgatt aagcttgcgg gcctctctcc tgcccaccag ccggatggtg acttgcgcat
     2221 cggtacgcaa ggccggcttg gcccaggacc tcatgcgttg ccgcgatggc aggccagtct
     2281 gtgcaatacc atactgcacg gaaagaatga gcaaggccgg cggcgagcgc accgttttcg
     2341 ctgccatgcc aatcagtcgg cctgacggac ttggtggcgt tcataggcct ggacgatgcg
     2401 ctggaccagt ggatgacgta ctacatcgtc tgcaagaaac tgggtaaagg caatgccgcg
     2461 tacatcgcgc aaaatgatgg cggcttccac cagaccgctc ttctggctct tcgccagatc
     2521 gatttgggtc acgtctcccg taacgaccgc acggcttccg aagccaatgc gggttagaaa
     2581 catcttcatt tgttccggcg tcgtattctg cgcttcatcc aggataatga aggaatggtt
     2641 cagggttcgc ccgcgcatga acgcaagcgg ggcaatttcg attgcggctc gttcgaaaag
     2701 cttggctacc ttctcaaacc ccatcaggtc gtagagcgcg tcgtagagcg ggcgcagata
     2761 cgggtccact ttttgcgcca ggtcgccagg caagaaacca agccgttctc cggcttccac
     2821 ggcaggccgc accagcacga tgcgctttac cgcatctcgt tcaaaagcat ccaccgcgca
     2881 tgcgacagcg agataggtct tgccggtgcc cgcggggccg ataccaaacg taatgtcatg
     2941 ttccctgatt tgccggatat attgcacctg ccgtggcgtg cggccgtgca ggtcgcgccg
     3001 tcgcgtctgg agcaccaatg cgtccgccgg tgcagtcggt gcggcgctac ccgctatctc
     3061 gatcaatcca agctgcacat cctcgagact cacgctgtca gtggcttgct gatagaaacg
     3121 ctgcaatgct tccgctgcga gccgtgacgg ttcgggcgca ccagagatgc tgaactgttc
     3181 tccacgccgc gcgattgcga catcgaaggc gctctcgatc tgcttgaggt tttcgtccag
     3241 tgcaccgcaa agattggcga gccgctggtt gtcgaccgga gtaaacgaga tatcgagggg
     3301 gttcaaacgg gttccgtgac gcgtacttcg ccgcggagag tgtgcgctaa tgcagcagta
     3361 atatgtacgt ccatgagctt gccgattaga cgcttcgatc cgggaaagtt caccacccgg
     3421 ttattgctgg tccggccgta gagttcatga agatttttgc gcgaagttcc ttcgaccaaa
     3481 atgcgttcga cttgaccaac catggcgttg ctgattgcct tggcgctcac atcaagagct
     3541 gcttgcagac gcgcgaggcg cgcctgcttg atttccagcg gaacctgttc agcgaagttt
     3601 gcagcggggg tgccgggccg gggactgtac atgaagctgt aactatcatc gaaatgaagc
     3661 tcttcgacta gtttcatggt ttcggcgaag tcttgttctg tttcaccggg aaaaccgacg
     3721 atgaaatccg atgcaattga gatgcctggc cgtgctgtgc gcaaacgccg gatgatcgat
     3781 ttgtattcca gtgacgtgta tccgcgtttc atggcagcca ggatccggtc ggacccggac
     3841 tgcacgggta gatggacatg atctacaagc tggggcaggt gccggtaaga atcgactaag
     3901 cgctgtgaga attcgcgagg atgcgatgtc gtataacgca aacgcttaat gcctggcatc
     3961 tctgcgagat actcgagcag cagagcgaag tccgccgttt cgccatcgtt catcttgccc
     4021 cgataagcgt tcacgttctg gcccaacaag gtgatctctt tcgttccctg atccgcgagc
     4081 ccggcgattt cggtaaggac atcgtcgaat ggcctggata cctcttcccc ccgggtgtag
     4141 ggaaccacgc agaagctgca atatttgcta cagccttcca tgatcgagac aaatgcagtg
     4201 cagccttcgt gtgttgcagc aggcaacgca tcgaacttct ctatttccgg aaaggagatg
     4261 tcaacttgcg aacggccgct tcgacggcgc gcatcgataa gctgcggtaa tcgatggagc
     4321 gtctgcggac cgaatacgac atccacaaac ggtgcgcgct cgacgatcgc ttgcccctcc
     4381 tggctcgcca cgcagccacc aactccaatg ataaggtctt gccgcgcctg cttcagcggg
     4441 cgcaaccgcc cgagggcgtg aaatacgcgc tcctgtgcat tttcgcggac agaacaggta
     4501 ttgaagagga taacgtccgc ctgagctgga tcctctgtcc tttcgaaagc ttcagaggca
     4561 tttaacacgt cggccatctt gtcagagtca tattcgttca tctggcaacc gtaagttctg
     4621 atatacagct tcttcattcg gctgccttat ttcaccagcg ggtgctgcgc ggcttcttcg
     4681 cgcgtcaaca gccatatccg ggacagatca ccactcgtat cgagcaaata catgactgga
     4741 gcggtttgaa tattcatcga ggccggcatg atgatgagat tctgctggtt atagagataa
     4801 ctgcccgctg ccagccggta gacgcgctcg cctattttca tcaatggata acggtacgcc
     4861 tgcatatccc cgcgtctggc ctgctgcggg aaatcgcgcg ccgcacaggt ctgcgttacg
     4921 caaagcaggg ccagcaacga cgccagcagc acatggagca attttttcat gggcaccatt
     4981 ccaaaggccg ccgccgggtt tgcgacgtcg tgctgaacga ttaggacacc tataacttga
     5041 cgcgtgattg tataacgccg actagaagag gatttgcgcc gcagcaaacc ctcttcttat
     5101 cgactttttg tgtggtggtg ataggtggac ttgaaccacc gaccccagca ttatgagtgc
     5161 tgtgctctaa ccaactgagc tatatcaccg gaagctgatc ctgcattcgc gctgatgtgc
     5221 tttcctccgg cggctccggg gagaggccat attttctgcc ccacggtaca ggcccgcaaa
     5281 atggaagcac cggatgaaag tgttttcaca tgtctttggc catcgatatc ttgcgcgctg
     5341 ttcgccccgc cctgcagggt gcggaagatt actgacgccc cttggggttg tcaaggcgag
     5401 agaggccagt ttgggccttc tgcgcggtaa actcttacca tgatcgtaga tgtcgcaatc
     5461 gtcggtggtg gaccggttgg cgccagtgtc gcgctggcat tggcgcctac atcgttcagc
     5521 attgcattaa tagaaccaag gcgtcctccg ctgctaccag ctgctggctt tgaccagcgc
     5581 gtgtatgcct tgaaccgccg cagtcgaagg ttcctcgagc gctgcgggat ctggcagcat
     5641 ttactgatgg atcgtatcgc accggtgcgt gaaatgcgcg tattcgggga ccaggcgtcg
     5701 caacttgatt tcagcgcgta tcgcaatggc gttccagaat tggcagcaat cgtcgaagaa
     5761 gccaatttgc agcaagcgct ccagcgcgcg ctctccagcc agtctaacct tgctgtgctg
     5821 gccggagttg cctgcctggg tgctacatgg gatagcgaaa aggcgactct atcgttgtcc
     5881 gatggacagc aaattgcagc aagcctggtg attgccgctg acggcgccga ttccgcgttg
     5941 cgtagcgccg ccggcatttc ggtgcgcgtc catgaatacg ggcagaccgg tgtggtcgca
     6001 aattttcgcg ccgggcaacc gcattgcgac acggcctttc aatggtttgt tgaaggaagc
     6061 gtgctggcac ttttgccatt gccagcgaac cacatgtcga tggtatggtc ggttcccaat
     6121 gcacaggctg gcgctctact gagcctgacc gccgaacaac tcgcagaaga agtgcaacgg
     6181 atgagcgaca acgtgattgg cctgcagccc acaggtgtgg cggcggggtt tgccttaaga
     6241 cgcatgcagg cagcacggct tattgcccca cgccttgccc tggtggggga cactgcccat
     6301 aatgtccatc ctcttgctgg gcagggtctt aatcttggat tcggcgatgc cgaatccctg
     6361 gcagccgtac ttgccacgag cggcactcac ggcgatccag gagcttacgc gttgttgcgc
     6421 cgatatgagc gcagcaggcg ggaagatcat ctggcaatgg aagtcgtcac tgaaggcctg
     6481 caattgctgt tcgccagcac tgttccagga atgaagcgtt tgcgtaatgc cgggctgcga
     6541 ctagtcaatc gcacggcgcc tttaaagcgc ttgctcgtga aacgagcgct ggggtaagtg
     6601 gtccaacgat gcctttcatc gtataactat tgggtttgtc ttctaacatg ccgattcgca
     6661 ctataagcat cttgttctca ttgctggcgt tggtcaccgg cgttgcattc gcggacgagg
     6721 cttcaattcg cgccgaattt gccaagaaat atccgcaagc caacatcgaa agtatttcca
     6781 agacaccgta cctcgggctg tacgaaatgc tggtcgatgg ggaagtcatc tacaccgatc
     6841 cggatttcac ttatctgatt gttggcagtc tcatcgagac caagaccaaa gttaatctca
     6901 cggatgcccg ccagagagaa atcgaagaga agaaactcaa gagcatggct ttcccttttg
     6961 aacagcttcc gttcgatctc gcaatcaaga aggtcaaagg cgatggaagc cgcaaagtcg
     7021 cagtgttttc cgatcccgat tgcccttact gtcgcaggct ggagaaggat ctggaaaaga
     7081 tctcaaacgc gacgatttac atcttcttgt tcccgatcga gcagttgcat ccgaaagcgc
     7141 ccgaaatgtc gcgtgcgatc tggtgcgctc ccgatcgcgt gaaggcctgg gatgagtaca
     7201 tgttgaaagg tgtcgtgccg aagtctccga agtgtgacac gcccgtcgac aagatcgtcg
     7261 cctatgggca gtccaaaaaa attaacggaa ctcccacgat cttctttgca gatggcaagc
     7321 gggttcccgg cgccattccg tccgaacggt ttgaggagct actcaaagca agcaatcccg
     7381 gcaaatcctg aaatcagatc gcccgccgcc gcctgagtct agaatgagaa cccagcgtaa
     7441 ccggctggga gggttaggtg cgcagggtcc ggcgaagcgt tccagggctt cctcaagcga
     7501 tactcaaccg tatatccgcg gcgattgtcc tttcagctgc ggtgcacgta ttactcgtgt
     7561 acggtttctc gcttactgaa ggcacgtcta caaccaagcc cagcgggatt atccaggcga
     7621 gactgcagcc aattccgtta tcagcgccgc tgcgatcaat agcacaagat acccacccgg
     7681 atttacccac tccccaggac gccataccaa atgtggtcgc atccgaaccg gtgtcggcgg
     7741 cacaaccggt agcgattcca gacgttcctg ttccagaacc acagcccagc aaggatcgcg
     7801 atgagtccag cgtcattccc gatctggtgc actatgcggc aagccagctc gatgtttatc
     7861 cgcgtgcgct gcaaacgata gcgccggctt acccgacagg cgcactggaa acgcaaactg
     7921 ccggctttgt gacgctgctc gtattgatcg atgaagttgg acgtgtcatg ggcaccactg
     7981 tagtggatgc tacacctgac ggcgtgttcg aacaggctgc gcagcaagct ttggcggaag
     8041 ccatttttta tcccgcgcag agagaaggcc acagtgtgcg cagtcggata ctggttaaag
     8101 tcgaatttga tcccagtgcg gcggccgcca tgcattgatc aatcagggcg gtggcaattc
     8161 cctcggctgc attccgcgtg gatagagaat ccacatggtg ccggtctgac gcatgcgtcc
     8221 ggcggactga gtcgcgtcat ctccgaatcc ccagaaaaag tcagcgcgga tcggaccttt
     8281 tattgcagtt ccggtgtcct gtgcaatcac cagacggttg agcggtcgca tagacagcgg
     8341 ggcagttgtg gacagaaaca ccggtgcgcc gagcggtgta gtcagcgggt cgaccgcgat
     8401 actgcgaccg ggcgtgagcg gaacgccttg agctccgatc ggcccagccg tcgatcctgg
     8461 cagttcgcga aagaatacgt aggccgggtt ttgctgtaac aactgcacaa ctttgccggg
     8521 gtttttggca gcccactgtt tgatgccctg catggatgcc ttatccagcg gaagctctcc
     8581 gcggtcgacc agcaatttgc cgaccgacac gtagggatga ccgttatgtt cggcaaaacc
     8641 gactcggagg atgtctccac cgggcagctt gatgcgtcca gagccctgca catgcaaaaa
     8701 aaacagttcg accggatcgt cgacccagac aatctccagt ccccgaaccg gggccagccc
     8761 gctctcgatt tctgcgcggc tgtaatacgg gacgattctt ttgtcttgca tccgcccccg
     8821 cagccggatg cccttgagct gaggattggt ctgcgccaga tcgattgcca gcagatcggg
     8881 aggaactgcg taaatcggaa agcgaaattc gcgaccgaaa tggcgactgc cgtacaacaa
     8941 gggctcgtag tagccggtca ccagtccttc ggtggcaccg gtcgcgttat gtaccagcca
     9001 gggcgcgaaa gaaga
//
LOCUS       DATMZQ010000140        10034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2872624, whole genome shotgun sequence.
ACCESSION   DATMZQ010000140 DATMZQ010000000
VERSION     DATMZQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872624"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VN496_04590"
     CDS             complement(<1..331)
                     /locus_tag="VN496_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXF16256.1"
                     /translation="MSRFWSAVVHGLTPYVPGEQPRLANLVKLNTNENPYGPSPRVLE
                     ALRAELGDSLRLYPDPISERLRQAIAVQHDVTAANIFVGNGSDEVLAHTFQALLKHEF
                     PILFPDVT"
     gene            complement(328..1629)
                     /gene="hisD"
                     /locus_tag="VN496_04595"
     CDS             complement(328..1629)
                     /gene="hisD"
                     /locus_tag="VN496_04595"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXF16257.1"
                     /translation="MTAINRLSSSEPEFEAKLAKLLAFESTQDAGVEKTVDSILREVR
                     SRGDDAVLEYTAKWDSIQANNLSALEISREDLDRALAELPAVRRYALEQAAARIRSYH
                     EHQTLRSWQYTEADGTTLGQKITPLDRVGIYVPGGKAAYPSTVLMNSLPARVAGVSEI
                     IMAVPTPYGERNQLVMAAASIAGVDRVFTIGGAQAIAAFAYGTQTIAAVDKIVGPGNA
                     YVAAAKRRVFGVVGIDMVAGPSEILVICDGTADPDWVAMDLFSQAEHDELAQAILLCP
                     NIQFIERVAASIQRQLKDMSRANVISASLNGRGALIETRDLDEACAIANRVAPEHLQL
                     CVEEPNRWAQKIRNAGAIFLGRYTSEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQK
                     RTSMIEVSIEGARKLGAIAAELAMGEGLPAHAKSAQYRIHR"
     gene            complement(1626..2273)
                     /gene="hisG"
                     /locus_tag="VN496_04600"
     CDS             complement(1626..2273)
                     /gene="hisG"
                     /locus_tag="VN496_04600"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXF16258.1"
                     /translation="MSLRIALSKGRIFEQTVPLLKAAGIVPAEDPDTSRKLIIPTNND
                     NVSVIIVRASDVPTYVQYGAADLGVAGKDVLLEHGGTGLYQPIDLDIAKCRMMVAVPE
                     KFDYEAAVRRGARLRIATKYLKTSREHFAAKGVHVDLIKLYGSMELAPLVGLADAIVD
                     LVSSGNTLRANQLKTVEEIMPISARLIVNQAALKLKRVEMQPLIDAFRAAALESA"
     gene            complement(2296..3549)
                     /gene="murA"
                     /locus_tag="VN496_04605"
     CDS             complement(2296..3549)
                     /gene="murA"
                     /locus_tag="VN496_04605"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXF16259.1"
                     /translation="MDKLVIQGGVPLTGEVRISGAKNAALPILCAALLTGDTLRVQNI
                     PHLRDVTTTLNLLGQMGVEVSLDEKLGVGLSAAKLHNLSAPYELVKTMRASILVLGPM
                     IARYGHARVSLPGGCAIGQRPVDLHIKGLQALGAQIDIDQGYILARADRLTGTHIVLD
                     LVTVTGTENLMMAAVLAEGITVLENAAREPEVYDLAECLNAMGARIKGAGTDMITIEG
                     VAQLHGATHRVMPDRIEIGTFLCAAAATRGEIMLKGTRANILDAVIGKLQEAGAHVEH
                     GEDWIRLSTNGSLRAVNLRTAPYPAFPTDMQAQFMALNAVAQGTAVVTETIFENRFMH
                     VQELRRLGADIEVEGNTAIVKGVGRLRGAKVMATDLRASASLVLAGLVANGETLVDRI
                     YHLDRGYECIEEKLSQLGARIKRAH"
     gene            complement(3564..3800)
                     /locus_tag="VN496_04610"
     CDS             complement(3564..3800)
                     /locus_tag="VN496_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HXF16260.1"
                     /translation="MVTPEQVKSYIEQNLACDHVEVSGDGHHFEAVIVSPEFRSRSRV
                     QQHQVVYKALGDRMREEIHALSMKTFTPEDWAAR"
     gene            complement(3813..4568)
                     /locus_tag="VN496_04615"
     CDS             complement(3813..4568)
                     /locus_tag="VN496_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF16261.1"
                     /translation="MGAFGTLFRKEITRFWKVSFQTVLAPVLTALLYLLIFSHVLEEH
                     VQVYPGVRYTAFLVPGLAMMSLLQNSFANSSSSLIQSKITGNIIFVLLPPISHFEFFS
                     AYVLASVVRGLMVGFGVFVITAIFVHVPMVNVPWVLAFALLGSGLLGTLGIVAGIWAE
                     KFDQLAAFQNFIIVPLTFLSGVFYSIHSLPVFWQDVSHFNPFFYMIDGFRYGFFGVSD
                     VSPLLSVSIVGGCLLALSFGTLSLLKRGYKLRH"
     gene            complement(4589..5491)
                     /locus_tag="VN496_04620"
     CDS             complement(4589..5491)
                     /locus_tag="VN496_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF16262.1"
                     /translation="MPPAIEIRGVSKAYGSVRALDGITLDILRGEFFALLGPNGAGKT
                     TLISILAGLARADSGILRVMGHDVVIDYRDARRSLGVVPQELVFDPFFTVRETLQFQS
                     GYFGLGRNNDWIDEVMARLDLSDRADFNMRALSGGMKRRVLVAQALVHKPPVIILDEP
                     TAGVDVELRQGLWQFIRQLNGDGHTIVLTTHYLDEAEALCSRVGMLKQGKLVALDSTH
                     NLLKGYTGAHLRLRLAHGSLPDSLKPMTATPPTNGEYLLALRDYAEVEAVLAELRKSN
                     LTVVFMELEQPDLEEVFVEIMRQD"
     gene            complement(5525..5809)
                     /locus_tag="VN496_04625"
     CDS             complement(5525..5809)
                     /locus_tag="VN496_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXF16263.1"
                     /translation="MIAREANRYFIEGPVTLQNVAQFLAEGATFEGTDLVVDFARVTT
                     VDSSAVSLILEWVRRFNNSGKKISFAHLTQSLRSLAELYGVIDLIPIAAD"
     gene            complement(5806..6453)
                     /locus_tag="VN496_04630"
     CDS             complement(5806..6453)
                     /locus_tag="VN496_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF16264.1"
                     /translation="MQKGIRALGTCFIAFLIAGSFVSRAPAEEVSPDALVRKTTEEVL
                     TIVREDKDIRAGDVTKIVQLIEQKVAPHFDFERMTRLAVGRPWREASPEQRDTLVKQF
                     RTLLVRSYSAAFTAYTNISVEYRPYKPSPGETETSVQTLIKLPGGAAPMTVDYDMAHT
                     PKGWKVYDVRVAGASLIINYRNLFAQEIQSGGIDGLIKSLIEKNSEKPQPPTAKK"
     gene            complement(6478..6960)
                     /gene="mlaD"
                     /locus_tag="VN496_04635"
     CDS             complement(6478..6960)
                     /gene="mlaD"
                     /locus_tag="VN496_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HXF16265.1"
                     /translation="MIMQRTTVDLWVGIFVTAGVAALFVLALKVGNASSVNMSDGYRV
                     IAEFDNIGGLKARAPVKSAGVVVGRVESIALDTRNFRASVALRIDKRYPFPRDSSASI
                     LTAGLLGEQYVGMDGGGDEQNLKDGDRLKITQSAIVLEKVIGQFLYGKAAETGTGKKE
                     "
     gene            complement(6957..7751)
                     /gene="mlaE"
                     /locus_tag="VN496_04640"
     CDS             complement(6957..7751)
                     /gene="mlaE"
                     /locus_tag="VN496_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HXF16266.1"
                     /translation="MLRALSHGLQLVGRRSINAVWRMGSGARFMGLVLLRSSPSFRRF
                     HLTIDELHFAGVLSLIIIIVSGLFVGLVLGLQGYETLKRYGSAEAVGTLVALALVREL
                     GPVVAALLFASRAGSAMTAELGIMKSTEQLSAMEMMAVDPFARVISPMFWGGFLSMPL
                     LAAIFSVMGIFGGYLITVVIIGVDSGAFWSQMQSSVDLRNDIINGVIKSTVFGAAVTA
                     IALFEGYDAPPTAEGVSHAVTRTVVTSALAVLALDFVLTAFMFTGV"
     gene            complement(7753..8556)
                     /locus_tag="VN496_04645"
     CDS             complement(7753..8556)
                     /locus_tag="VN496_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF16267.1"
                     /translation="MSPDNLVEFAHVDFSYDRRPILQGVDMAVPRGKVVAIMGGSGSG
                     KTTLLRLIGGQLKPDRGDVLVESQTLQKMPHAQLYALRRKMGMLFQFGALFTDLSVFD
                     NVAFPLREHTSLPASMIRDLVLMKLHAVGLRGAESLKPSELSGGMARRVALARAIALD
                     PMLIMYDEPFTGLDPISVGIIGNLIRRLNDALGATSIVVTHDVQESLRIVDYVYFLSG
                     GRIVSHGTPREVLESNEPFVYQFVHGKADGPVPFHYGAPEYGKDLGVTS"
     gene            8780..>10034
                     /locus_tag="VN496_04650"
     CDS             8780..>10034
                     /locus_tag="VN496_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXF16268.1"
                     /translation="MDTPSGQKPELLIVDDDPLIVDTLTFVLGKDYSVRAAGSREQVK
                     SLLRQLDVSPELALVDLGLPPSPHRPDEGFHLIADLLAHCPTMKILVLSGQNDEANAR
                     YARTLGAAEFVAKPCEPEVLKSMLAKMRRMAEVERSSVKVDTGIVGAGHAITKLHQQI
                     AQYATAPFPVLVEGESGSGKELVAQSLHRQGPRDGKPYLALNCAAISPSLVEPTLFGY
                     VKGAFTGATSSRSGYFEDAHEGTLFLDEIGELPLELQSKLLRVLENGEYQRVGETQKR
                     VSNARVIAATNRDLRQEVRASRFRADLYHRLSVFSISVPPLRDLADDKAKLLNHYSAL
                     YAQQSNVPAFRLDRSSLDLWMQYGFPGNVRELRNIVIRLTTKYPGQTINSEQLLGEFD
                     WEEPMATPVQTSQTGDFKSSVAAARV"
BASE COUNT         2379 a         2846 c         2629 g         2180 t
ORIGIN      
        1 aagtaacatc cggaaacagg atgggaaact catgcttgag caaggcctga aaagtgtgcg
       61 caagcacctc atccgatccg ttcccgacaa agatgttggc ggcggtcaca tcgtgctgca
      121 cagcaatagc ttgtctcagc cgctcgctaa taggatcggg ataaaggcgc aacgaatcgc
      181 ctagctccgc tcgtaacgct tccagcacac ggggactcgg tccgtaggga ttttcgttgg
      241 tgttgagctt gaccagattt gccagcctgg gttgctctcc gggaacgtaa ggcgtcaggc
      301 cgtgcaccac ggcgctccag aatcggctca tcgatggata cgatactgtg cggattttgc
      361 gtgagccggc aaaccttcac ccatggccaa ctcagcggcg atagcgccaa gcttgcgagc
      421 gccttcgatt gatacttcaa tcatgctggt gcgcttctga aaatcgtaaa cgcccaaagg
      481 cgacgagaaa cgagcagtcc gtgaagtcgg caatacgtgg tttgggcctg cgcagtagtc
      541 gcccaacgcc tctgaggtat aacggccaag aaagattgcg cccgcattgc gtatcttttg
      601 cgcccagcgg ttgggttctt cgacacacaa ttgcaagtgt tccggggcga cccggtttgc
      661 gatcgcacac gcttcatcca aatcacgggt ctcaatcaac gcgccgcgtc cattcagcga
      721 agcactgatc acgtttgcgc gggacatgtc tttcagttgc cgctggatgc ttgcggcaac
      781 cctctcgata aattgtatat ttggacagag caaaattgct tgcgccagct cgtcatgctc
      841 cgcctgggaa aacagatcca tggcaaccca atcgggatcg gctgtaccgt cgcagatcac
      901 cagtatctca gatggaccgg ctaccatatc tatgccgacg accccaaaaa cacggcgttt
      961 ggcggcagcg acataagcat ttccagggcc gacgatcttg tccactgccg caatggtttg
     1021 agtgccatat gcaaatgcag caatggcttg cgcaccacca atcgtaaata cgcggtcaac
     1081 ccccgcgatg ctggctgctg ccattaccag ttggttacgc tcaccataag gcgtcggcac
     1141 agccatgatg atttctgata cgcccgctac ccgcgcaggc agcgaattca tcaataccgt
     1201 agacggataa gccgctttcc cacccggaac gtaaatacca acgcgatcca gaggcgtaat
     1261 tttttgtccc agcgtggtgc cgtccgcctc ggtgtattgc caggatcgca acgtctggtg
     1321 ttcgtgatag ctacggatgc gggctgcggc ctgctcaagt gcgtaacggc gcactgcagg
     1381 caactcggca agcgcccggt cgaggtcctc gcgagagatt tcgagcgcgg acaaattatt
     1441 agcctgtata gaatcccatt tcgcggtgta ttctagaacc gcgtcgtcgc cgcgggaccg
     1501 cacctcgcgc aatatggagt cgacggtttt ttcgacgcca gcgtcctgag tgctttcgaa
     1561 cgccagcaac tttgccagtt tcgcctcgaa ttccggctcg ctcgaggaca gcctattgat
     1621 agccgtcatg ctgactccaa tgctgcggca cgaaacgcat cgatcagtgg ttgcatttcc
     1681 acgcgcttca gtttcagagc ggcctggttg acgatgaggc gggcgctgat gggcattatc
     1741 tcttctacgg ttttgagctg gttcgcacgt aatgtattcc cgcttgagac aagatccaca
     1801 attgcatcag ccaatcccac caaaggggca agctccatgg agccataaag cttgatcaga
     1861 tcgacatgca cgcccttcgc cgcgaaatgc tccctcgagg ttttgagata cttggtcgca
     1921 atacgaaggc gggccccacg tcgcacggca gcttcgtagt caaatttttc gggcaccgcg
     1981 accatcatgc ggcacttggc gatatcgaga tcaatcggtt gatagagtcc ggtaccgccg
     2041 tgctcgagca atacgtcttt gcctgcaacg cccaagtcgg ccgcgccgta ctgtacataa
     2101 gtgggcacat ctgaagcacg cacgataatg acagaaacat tgtcgttgtt ggtcgggatg
     2161 atgagcttgc gcgaagtgtc cgggtcttca gccgggacaa tgcccgccgc cttcagcagc
     2221 ggcactgttt gttcgaatat gcgccctttt gaaagggcga ttcttagact catttttttc
     2281 cgtcacaccg cgacgtcaat gtgcccgctt gattcgagcg cccaattgac tcagtttttc
     2341 ttctatgcac tcgtagccgc gatccaaatg gtaaatgcgg tccacgaggg tttctccgtt
     2401 tgcgaccagg ccagccaaca ccagactcgc tgatgctcgc aggtccgtgg ccatgacttt
     2461 ggcgccgcgc aaccgcccaa ctcccttgac gatggcggta tttccctcaa cctcgatgtc
     2521 tgcgcccaac cggcgcaatt cctggacgtg catgaatcga ttttcaaata ttgtttcggt
     2581 aaccacggcg gttccttgcg ccaccgcgtt tagcgccatg aactgtgctt gcatatcggt
     2641 agggaatgcc gggtagggcg ccgtgcgcaa attgacggcc ctaaggctgc cattggtgga
     2701 caaccgtatc cagtcttcac cgtgctcaac atgagcaccc gcttcttgca acttgccaat
     2761 aaccgcgtcc agaatgttgg cgcgcgtccc tttcaacatg atttctcccc tggtggctgc
     2821 cgccgcgcag agaaatgtgc ctatttcgat tcgatctggc atgacgcggt gcgtggctcc
     2881 gtgcaactgt gctacgccct cgatggtgat catgtcagta ccggcgcctt tgatccgtgc
     2941 acccatggcg ttcagacatt ccgccaagtc gtagacttct ggctcgcgcg ccgcattttc
     3001 aaggaccgtg atgccctctg ccaagaccgc agccatcatt aggttctcgg ttccagttac
     3061 ggtcaccaaa tcaaggacaa tatgcgttcc cgtaagccga tcggcacgag cgagaatgta
     3121 accttgatct atgtcgattt gtgcaccgag ggcttgcaaa cccttgatgt gcagatcgac
     3181 cggccgttgc cctatcgcgc atccaccggg caaagatact cgcgcatggc cataacgcgc
     3241 gatcatcggc ccgagcacca aaattgaagc ccgcatggtt tttacgagtt catacggtgc
     3301 agacaagttg tgaagcttcg ccgcggacag tccgacgccc aatttctcgt caagcgagac
     3361 ctccactccc atctggccga gaaggttaag cgtagtggtg acatcgcgca ggtgaggaat
     3421 gttctgtact ctcaacgtgt cgcccgtgag cagcgccgcg cagagaattg gaagtgccgc
     3481 attcttggca ccggaaatac gtacttcccc ggttagcggc acgccgccct ggatgacaag
     3541 cttatccact tggcaggtac gggctagcgc gcagcccagt cttccggggt aaaagtcttc
     3601 atggacagcg cgtgaatctc ttcgcgcatg cggtctccga gcgctttata gaccacttga
     3661 tgctgctgaa cccgcgaacg gctccgaaac tccgggctta cgattacggc ctcgaaatga
     3721 tggccgtcgc ccgatacctc gacatggtcg caggcgagat tttgctcaat atacgacttc
     3781 acttgttctg gagtaaccat aaccactttt catcaatgac gaagtttgta gccccgttta
     3841 agcagcgaca aagtcccgaa tgataaagcc aaaaggcagc cacccacaat tgagacgctg
     3901 agcaagggcg agacatcgga gacgccaaaa aacccgtaac gaaagccgtc aatcatgtaa
     3961 aaaaaagggt tgaagtgcga gacgtcttgc cagaatacgg gcaatgagtg tatcgagtag
     4021 aagactcctg acaggaatgt aagcggaacg atgatgaaat tttgaaacgc cgctaactgg
     4081 tcgaactttt cagcccagat accggcaaca atgcctaatg taccgagcaa acccgaaccg
     4141 agcagtgcga acgctagcac ccaggggacg ttcaccatcg gaacatgcac gaaaatagcg
     4201 gtaatgacaa ataccccgaa gccgaccatc aagccgcgaa ctacggaagc cagtacgtaa
     4261 gcggaaaaaa attcgaagtg cgagatcggt ggcaggagca cgaaaatgat gttgccggtg
     4321 atcttggact ggatgagact ggaggagctg ttggcgaagg agttttgcaa caacgacatc
     4381 atggccaggc caggcacaag aaacgcggta tagcgtaccc cggggtaaac ttgaacgtgt
     4441 tcttccagca catgggagaa aatcagcaga taaagcagcg cagtcagtac cggcgcgagc
     4501 acagtctgaa aactaacttt ccagaatcgc gtgatttcct tgcggaacag cgtgccgaat
     4561 gcgcccatgg gggcgaagga ctccatggtc agtcctgtct catgatctct acgaatactt
     4621 cttcgagatc cggctgttcc agttccataa ataccacggt cagattgctt ttccggagtt
     4681 cggcgagtac ggcttcgacc tccgcgtagt cgcgcagggc gagcaggtat tctccgttgg
     4741 tgggaggtgt tgcagtcatc ggcttgagcg agtcaggcaa actgccatgt gccaggcgca
     4801 ggcgcaagtg ggcgccggtg tagcccttaa ggagattatg cgttgaatca agcgctacga
     4861 gtttgccctg cttcagcatt ccgactctgc tgcaaagcgc ctctgcttcg tcaagatagt
     4921 gggttgtgag gacaatagta tgaccatccc cattcaattg acgaatgaac tgccacagac
     4981 cttgacgcaa ttccacatcg acgcccgcag tcggctcgtc cagaatgatg acgggtggct
     5041 tgtgcaccag cgcctgagcc acaagcacgc ggcgcttcat gccgccagat agcgctcgca
     5101 tattaaaatc cgccctgtcg ctgaggtcca gccgggccat cacttcatcg atccagtcat
     5161 tatttctgcc gagtccgaaa taaccggact ggaactgaag ggtttcccgt accgtaaaaa
     5221 aaggatcaaa gaccagttct tgcggcacca ctccgagcga ccgccgcgca tcgcgataat
     5281 cgataacgac gtcatggccc atcacgcgaa ggatgccgct atcagcgcga gccaatccag
     5341 ccagaatgct aatcagggta gttttgcccg ctccgttggg accaagaagt gcgaaaaatt
     5401 cccctctcaa gatgtccagc gtaatcccat cgagcgctcg cacgctgcca taggccttcg
     5461 aaacgcccct tatctcgatg gccggcggca cgaaagacca ctggatttgg cgttcaacaa
     5521 gacactagtc ggccgcaatc ggaattagat caatgacccc gtaaagctcg gccagactgc
     5581 gcaaactctg agtgagatgg gcgaaggaaa ttttcttgcc gctattgttg aatcgacgaa
     5641 cccattccag aatcaggctt actgcagaag aatcgacggt tgtgacccgg gcaaaatcga
     5701 caactaaatc tgtgccttca aatgtcgcac cctcggcaag gaactgcgca acattctgga
     5761 gagtgaccgg accttcgatg aagtagcggt ttgcttctct agcgatcatt ttttagccgt
     5821 gggaggttgc ggcttttcac tattcttttc gattaagctc ttgatcagac catcgatgcc
     5881 gccgctttga atctcctggg caaacaggtt ccgatagttg atgataagac tcgctccagc
     5941 cacccggacg tcatacactt tccaaccttt tggcgtgtga gccatgtcgt agtctacagt
     6001 catgggagcc gccccaccgg gtaatttgat aagcgtctgt actgatgttt cagtttctcc
     6061 tgggcttggc ttgtacggtc gatattcgac tgagatattg gtgtatgccg tgaacgcggc
     6121 agaataagaa cgcaccagta aagtacgaaa ttgctttacc aaagtatcgc gctgctcggg
     6181 cgaagcttcc ctccacggac ggcccacggc caagcgggtc atacgctcaa aatcgaaatg
     6241 cggggcaact ttctgctcaa tcagttggac gatctttgtg acatcgcccg cacgaatgtc
     6301 cttatcctca cgcacgatcg tgagcacttc ctcggtggtc ttgcgaacca gtgcatcggg
     6361 ggatacttcc tcggcgggcg cacgactgac aaatgaaccc gctatcaaga aagcgataaa
     6421 acaagttcca agtgctctga tacctttctg catggtgatt tcctctttga acctttgcta
     6481 ttcctttttc cctgtaccag tctctgcagc tttgccataa aggaattggc ctattacctt
     6541 ttccagaaca atcgcggatt gagtaatctt caagcgatcg ccatccttca aattctgctc
     6601 gtccccgccc ccatccatgc cgacatactg ttctccgagc aagccggccg ttaagatcga
     6661 cgcggaggaa tcccgcggaa agggatagcg tttatctata cgtaacgcca cactcgcgcg
     6721 aaagttacgc gtatcgagtg caatgctttc cacacggcct acaacgactc ctgcactctt
     6781 taccggagcc cgcgccttca atccgccgat gttgtcgaat tctgcaatga ccctgtagcc
     6841 gtcggacatg tttacgctgc ttgcgttccc gactttcaac gccagaacaa acagcgctgc
     6901 cacgccagcc gtgacaaaaa tgcctaccca aaggtcaact gtagtccgtt gcataatcag
     6961 actcccgtaa acataaatgc cgtaagcaca aaatcaagcg cgagaacggc tagggcggat
     7021 gtgacgacgg tccgcgtaac agcatgggat acgccttccg cggtcggcgg tgcgtcgtat
     7081 ccctcaaaaa gagcaatcgc cgtaacagcg gctccgaata cagtactttt gatgaccccg
     7141 tttattatgt cattgcgcaa gtcaacactc gattgcatct gtgaccaaaa cgcacccgaa
     7201 tctacgccga ttattacgac cgtaatgagg taaccgccga atattcccat tacgctgaat
     7261 atcgcagcga gtaacggcat cgacaggaag ccgccccaga acatggggct gatcaccctg
     7321 gcaaacggat cgaccgccat catttccatc gcggacaact gttccgttga tttcataatg
     7381 ccaagctcgg ctgtcattgc cgaaccggca cgactggcaa acaacagggc agcgaccacc
     7441 ggacccaatt cccgaactaa tgcaagcgca accaatgtcc cgaccgcttc ggcggagccg
     7501 tagcgcttga gggtttcata accctgcagc cctagcacaa ggccgacgaa caagcccgaa
     7561 acgataatga tgattaggga aagtacgcct gcaaaatgca gttcatcgat ggtcagatgg
     7621 aaccgccgaa acgaaggact ggatcgaagc aatacaagtc ccatgaatcg ggccccggaa
     7681 cccattctcc acaccgcgtt tatggagcgc cggcccacca gctgcagacc gtgcgaaagt
     7741 gccctcagca tctcagcttg ttactcccag gtccttgccg tactccggcg cgccataatg
     7801 gaacggcacc ggcccatcag cttttccgtg gacgaactga tatacgaagg gctcattaga
     7861 ctcgaggact tctcggggcg taccgtggct gacaatacgt ccgcccgata aaaaatagac
     7921 gtaatcaaca atcctcaacg attcctgtac atcgtgcgtg actactatgg aggtcgcgcc
     7981 tagcgcatcg ttgagccggc gaatcagatt gccaatgatg ccaacggaaa ttgggtccag
     8041 gccagtgaac ggctcgtcgt acatgatgag catcggatcg agcgcgatgg cccgagcaag
     8101 tgccacgcgc cgcgccattc ctcccgacaa ttccgaaggt ttcagcgatt cagcacccct
     8161 caaaccgacc gcgtgcagct tcatcagcac caggtcgcga atcattgatg ccggtagact
     8221 cgtatgttcc cgcagcggaa atgccacatt gtcgaataca ctaagatcag taaacagggc
     8281 gccaaactgg aatagcatcc ccattttcct gcgcagtgcg tatagctggg cgtgtggcat
     8341 tttttgtagc gtttgcgatt caaccaggac atcgccgcga tcgggtttaa gctgcccgcc
     8401 aatcaagcgc agcagcgttg tcttgccgct accgctgccg cccatgattg ccacgacctt
     8461 tccgcgcgga acagccatgt ccactccctg cagaatgggt cgtcgatcat atgaaaaatc
     8521 gacgtgcgcg aattcgacga ggttatcggg cgacatggcg aaaattggcg cctaaaacag
     8581 gacgcactgt aacgggcgtg gcatcatcgc cgtactccgc cagtttcgga ttggactcaa
     8641 acaattcctc ttaggttgct taacggaaat ccagtttacg aagtgcgtgt gcggacgaaa
     8701 cctataatta gtatgaacaa tcaagccctt ctggcacaat aacacgaacc tcacaaagag
     8761 tcagcggccc aattagaaca tggatacacc gagcggacag aaaccggaac tgctgatcgt
     8821 cgacgatgat ccgctgatcg tcgatacctt gactttcgtg ctcggcaagg actacagcgt
     8881 ccgagcggcc ggttcgaggg agcaagtgaa aagtctgctt cgccagctgg acgtctcccc
     8941 ggaacttgct ttagtggatc tcgggcttcc tccaagtccg catcgtcccg acgaaggttt
     9001 tcatttgatt gcggatttgc tggcgcattg cccaacgatg aaaattctcg ttctctccgg
     9061 acagaacgac gaagcaaacg cccgctatgc ccgtacgttg ggcgcagcgg aatttgtcgc
     9121 caagccatgc gagccagaag tactaaagtc catgctggcg aaaatgagac gcatggcgga
     9181 ggtcgagcga tcctccgtca aggtggatac cggtatcgtg ggcgccggtc acgcaataac
     9241 gaaattgcat cagcaaatcg cgcagtacgc taccgcccct tttcctgtgc tggtagaggg
     9301 ggaatcgggc agcggcaagg agctggtggc gcaatcgctc caccggcagg ggccgcgcga
     9361 tggaaagcct taccttgcgc taaattgtgc tgctatttcg ccctccctgg tcgagccgac
     9421 gttgttcggc tatgtcaaag gcgcattcac tggcgcgact tcatcgcgct cgggctattt
     9481 cgaagacgcg catgaaggaa cgctgttcct tgatgaaatt ggcgaattgc ctcttgagct
     9541 gcagtcgaag cttttgcgcg tattggaaaa cggtgaatac cagcgtgttg gcgaaacgca
     9601 aaaacgggta tccaacgcac gcgtaatagc cgcgacgaat cgggacttgc gtcaggaagt
     9661 gcgcgcttca cggttcagag cagatctcta tcaccggctt tcggtatttt cgatatcagt
     9721 tccgcctttg cgcgatttgg cagacgacaa ggcgaaatta ctcaatcact attctgcact
     9781 gtacgcgcaa cagagtaacg ttcccgcctt caggctggat cgcagttcat tagacttgtg
     9841 gatgcaatat ggatttcctg gaaatgtgcg cgaattgcgc aatatcgtaa tcaggctgac
     9901 gaccaaatac cccgggcaga ccatcaatag cgagcaatta ttgggcgagt tcgactggga
     9961 agagccaatg gcgacaccag tccagacgag ccaaaccggg gactttaaat cttccgtggc
    10021 cgccgctcgg gtcc
//
LOCUS       DATMZQ010000141         5174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_747869, whole genome shotgun sequence.
ACCESSION   DATMZQ010000141 DATMZQ010000000
VERSION     DATMZQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747869"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VN496_04655"
     CDS             <1..220
                     /locus_tag="VN496_04655"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXF16269.1"
                     /translation="GDIIELDVPGRKLTLQVSDAELAKRRAAWKPHERKYARGYGKIF
                     SQHVSQADKGCDFDFLEGTAPIPEPEIH"
     gene            complement(249..716)
                     /locus_tag="VN496_04660"
     CDS             complement(249..716)
                     /locus_tag="VN496_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF16270.1"
                     /translation="MTDSKWKYHGVRVVHSTELDSNTAQTPGMNRAAAITHARCGAEK
                     LWAGTVVIHPKAKTGAHHHGPVESVIYVVSGRARMRWGDRLEFMAEAGPGDFIHVPPF
                     VPHQEINASDTEPLSCVLCRSGQEPVVVNLDIPAAERPEQVHWVDNIHPAPTR"
     gene            complement(731..1027)
                     /locus_tag="VN496_04665"
     CDS             complement(731..1027)
                     /locus_tag="VN496_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16271.1"
                     /translation="MNSKLRRFLLYYLPLLANMGLIVYLVWQFNFTPYLRRQGEFQIF
                     SVLYAIGGLVVALSGITAFIHASAYQHGPRGLFALALVNTIIPTVLLLAVLQLR"
     gene            complement(1058..3898)
                     /locus_tag="VN496_04670"
     CDS             complement(1058..3898)
                     /locus_tag="VN496_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXF16272.1"
                     /translation="MAGDVLFDAFSRGRYSTDASIYQVEPVGVVIPRTEDEARTALQI
                     AVDEGVPILPRGAGTSQCGQTVGAALVIDCTKYLHDVISIDPEQHVATVQPGLVLDHL
                     NARLRAHNLWFPVDVSTSAQATIGGMTGNNSCGSRSVRYGNMVHNVLGVDAWLSSGEE
                     FRFGPSTALGQGPAGYRALVEKLHTIVRRETAEIQARWPSVLRRVQGYNLDMIQPERQ
                     GQTLAHNLAHLLVGSEGTLAFFRRIQLQLSVIPTHKVLGVVHFPTFYQAMDLTRHIAT
                     LDADAVELVDRTMIDLARGNPAFRPVVDRFLQGEPEAILLVEFAGEDKTALLARLARL
                     NELMADLGLPGSVVQIVESALQKQIWEVRKAGLNIMMSMKGDGKPVSFIEDCAVPLEH
                     LAEYTDRLTQVFEKHGTRGTWYAHASVGTLHVRPVLDMRRDGAEKMRAIAEEACALVK
                     EFKGAFSGEHGDGLVRSEWIEPILGSRLTGALAEVKALMDPKGLMNPGKIVHPERMDD
                     RSLFRYKPGYQAQPIETVLDWSAWGGFAPAVEMCNNNGHCRKFDAGTMCPSFRATGDE
                     QHLTRGRANTLRLAISGQLGAQALVSDAMRQTLELCVSCKGCRRECPTGVDMARMKIE
                     FQHHYGKRHGLSLRQKLIAHLPHYAAIASKFAPLLNLRDRVPGAPWLTEKIFGLSARR
                     SLPRWSSGRLPNSRRGQREKKVVLFIDTFNRYFEPENACAATDVLQAAGYQVDIAAPV
                     DQGRPLCCGRTFLAAGLVAEAKAEARRMLQSLRPYVEQGVAVVGLEPSCLLTLRDEYL
                     AMGLGEEAVRLSARAMLFEEFLAAQADAGSLGLELAPLSKSALLHGHCHQKAFDAMPA
                     VSKVLALIPDLRVQTIESGCCGMAGSFGYEAEHFDVSLKMAELSLLPAVRQANADTIL
                     VADGTSCRHQIRDGSGRAAVHVAQLLKMALRQ"
     gene            4099..>5174
                     /locus_tag="VN496_04675"
     CDS             4099..>5174
                     /locus_tag="VN496_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXF16273.1"
                     /translation="MTTRSGRHFLQIPGPTNVPDRVLRAMDRPTIDHRGPQFAQLGRE
                     VLEGLKLVFRTSSPVIIYPSSGTGAWEAALVNTLSAGDRVLMSETGQFATLWQKLATR
                     LGLTIDFIPGDWRHGANAETIEHKLAEDRQHAIKAVCVVHNETSTGVVTRIPPVRKAI
                     DSTGHPALLLVDTISSLGSIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNAISAKALAA
                     SKRARLPRSYFSWDEMLGPNRSGFFPYTPATNLLYGLREALQMLQEEGLEHVFSRHAR
                     HAEATRRAVRAWGLEVLCAEPEEYSNTLTAVLLPAGQDADKVRELILERFDMSLGAGL
                     GKLQGKIFRIGHLGDFNDLTLAGT"
BASE COUNT         1090 a         1597 c         1468 g         1019 t
ORIGIN      
        1 cggcgacatc atcgaactgg acgtgccggg caggaaactg acgcttcaag tttcggatgc
       61 cgagttggca aagcgccggg ccgcatggaa gccgcatgag cgaaaatatg ctcgcggcta
      121 tggcaaaatt ttttcgcagc atgtgtcaca ggctgacaaa ggctgcgatt ttgattttct
      181 ggaaggcact gctcccatac cggagccgga aattcactga tatccgcagc gaaagcagct
      241 gggctcgctc agcgtgtagg cgcagggtga atgttgtcta cccaatgcac ctgctctgga
      301 cgctcagccg cgggaatatc aagattcacg acgaccggtt cctgcccgct cctgcacagc
      361 acgcatgaca gtggttctgt gtcagaggca ttgatttcct gatgaggcac aaaagggggc
      421 acatggataa aatcgccggg ccccgcttcc gccatgaatt caagccggtc gccccagcgc
      481 atgcgcgccc ttccactgac gacataaatc acgctctcga ccggaccgtg atgatgcgcc
      541 ccggtctttg ccttgggatg gatcacgacc gttccggccc ataatttttc cgctccacag
      601 cgcgcgtggg taatcgctgc tgcgcgattc atgccgggag tctgtgccgt attggagtcc
      661 agctccgtgg aatggacgac gcgtacgccg tgatatttcc acttcgaatc ggtcatggtt
      721 gttcctttgt tcagcgtagc tgaagcacag ccaacagcag cacggttgga ataatggtgt
      781 tgaccaatgc cagcgcaaac agcccgcgcg ggccgtgctg atatgcagaa gcatgaatga
      841 aagccgtgat acccgacagg gcgaccacca atccaccgat cgcgtacaac accgaaaaaa
      901 tctgaaattc tccctgacgc cgcagatacg gcgtgaaatt gaattgccag accaggtaga
      961 caatcaagcc catgttcgcc agcagcggca ggtaatagag cagaaagcgc cttaatttac
     1021 tgttcaaatt ctttccttct aaaatatgcg gcgatggtta ctgtcgcaac gccattttta
     1081 acaactgcgc gacgtgaacc gccgcgcggc cggatccgtc acgaatctga tgacggcaac
     1141 tcgtgccgtc cgcaaccagg atggtatcgg catttgcctg gcgcaccgcc ggcagcagcg
     1201 acaactccgc catcttgagg ctaacgtcaa aatgttccgc ctcataaccg aaactgcctg
     1261 ccatgccgca acaaccggat tcgatggtct gcacacgcaa atccggaatc agggccagta
     1321 ctttgctgac agccggcatc gcatcgaatg ccttctggtg gcaatgtccg tgcaacaagg
     1381 ctgacttgga aagcggcgcc agctcaaggc ccaacgagcc tgcgtccgcc tgtgccgcga
     1441 ggaattcctc gaacagcatt gcgcgtgcgg aaagccggac cgcttcttcg ccaaggccca
     1501 tcgccagata ttcgtcccgc aaagtcaata aacacgacgg ctccagtcca acgacggcga
     1561 cgccctgctc gacgtaaggg cgcagcgact gcagcatgcg ccgtgcttcc gccttcgctt
     1621 ctgcaaccag ccccgcggcc agaaatgtcc tgccgcagca cagcggccgg ccctggtcca
     1681 ctggcgccgc gatgtcgacc tggtagcccg ctgcttgtag cacgtccgtt gccgcgcagg
     1741 cattttccgg ttcgaaatag cggttgaacg tatcgatgaa caggactact tttttttctc
     1801 gctgtccgcg cctggaattg ggaagccgtc cactgctcca gcgcggcaag ctgcgccgcg
     1861 cactcagtcc aaaaattttc tcggtcagcc atggtgcgcc cggaactcga tcacgcaaat
     1921 tcagcagcgg cgcgaatttc gatgcgatgg cggcatagtg cggcagatgt gcgatcagtt
     1981 tctgccgcag ggaaagccca tggcgcttgc catagtgatg ctggaattcg attttcatgc
     2041 gcgccatgtc gacgccagtc ggacactcgc ggcggcagcc tttgcagctg acgcacaatt
     2101 ccagcgtctg ccgcatggca tccgatacca gcgcctgcgc accgagctga ccggaaatcg
     2161 ccagcctgag cgtgttggca cggccgcgcg tgagatgctg ctcgtccccg gtcgcacgaa
     2221 aagacgggca catggtgccg gcatcaaact tccggcaatg gccattgtta ttgcacatct
     2281 ccaccgcggg cgcaaagcca ccccaggccg accaatcgag taccgtttcg atgggctgcg
     2341 cctggtagcc cggcttataa cgaaacaggc tgcggtcgtc catacgctcg ggatgcacga
     2401 tcttgcccgg gttcatcaac cctttcgggt ccatcagcgc tttgacttcc gcaagggccc
     2461 cagtcagacg ggatcccagt atcggctcga tccactcgga gcgcaccagt ccgtcgccgt
     2521 gttcgcccga aaaggcgccc ttgaattcct tgaccagcgc acaggcctct tcggcaatgg
     2581 cgcgcatttt ttctgcgccg tccctgcgca tgtcgagcac gggacgcaca tgcagcgtgc
     2641 cgaccgaggc gtgcgcatac cacgtgcccc gggtaccgtg cttctcgaac acttgcgtga
     2701 gccggtcggt atattcagcg agatgttcga gcggtactgc acaatcttca atgaaagaca
     2761 ccggtttgcc atcacccttc atcgacatca tgatgttgag ccccgccttg cgcacttccc
     2821 agatctgctt ttgcagcgcg gactcgacga tctgtaccac actaccgggc agaccaaggt
     2881 cagccatgag ctcgttcagg cgcgcaaggc gtgcaagcag ggcagtcttg tcttcgcccg
     2941 caaattccac cagcaggatc gcttctggtt cgccctgcaa aaaacggtcg accaccgggc
     3001 gaaacgccgg gttgccgcgc gcaaggtcaa tcatggtgcg gtcaaccagc tcaaccgcgt
     3061 ccgcatccag cgtggcaatg tgccgtgtca gatccatcgc ctggtagaag gttgggaaat
     3121 gcactacacc caataccttg tgcgtcggga taacagagag ctggagctgg atgcgacgga
     3181 aaaaggccag cgtaccttct gatccgacca gcagatgcgc aagattgtgg gccagcgttt
     3241 gcccctggcg ctcaggctgg atcatgtcca ggttatatcc ctgcacccga cgtaacaccg
     3301 acggccagcg cgcctggatt tctgcggtct cacggcgcac gatcgtgtga agcttctcta
     3361 ctaatgcacg atagcctgcg gggccctgac ccaatgccgt gctggggccg aaacgaaact
     3421 cctcgccact actgagccag gcgtccacac cgagcacgtt atgcaccatg ttgccgtaac
     3481 gtacggaacg cgagccgcag gagttgttgc ccgtcatgcc gccgatggtg gcctgggccg
     3541 aagtcgatac atcgaccgga aaccacaggt tatgcgcacg caggcgcgca ttcaggtgat
     3601 cgagtaccag gccgggctgc accgtggcta cgtgctgttc cggatcaatg ctaatgacat
     3661 cgtgcaagta tttagtgcaa tcgatgacca gcgctgcccc gaccgtctgg ccgcactgtg
     3721 aagtgccggc cccgcgaggc agaatcggca cgccctcgtc cacggcaatc tgcaacgccg
     3781 tacgcgcctc atcttcggta cgcggtatga ctacaccaac cggttccact tgatagattg
     3841 acgcgtccgt ggaataacgg ccgcgtgaaa atgcatcgaa cagcacgtcg ccggccatgt
     3901 tcgctcgcag tcgccgttcc aaggggctac gagcgcccgg gggcgcatga aatcgtaagg
     3961 gtattgccgg agcgttcacg ctaaaacagt ttaaggtctc ggaacagaag ccctgaatga
     4021 tacccgaggg cccctgcgga cgcgcttgtt agggcgtacg atcttaagta ggctcaattc
     4081 tttaatcgga tcccgaccat gacgactcgc agcggacgac acttcttgca gataccgggc
     4141 ccgaccaacg taccagaccg cgtgctgcga gccatggacc ggccaaccat cgatcatcgc
     4201 ggtccgcaat ttgcgcagct cgggcgcgag gtgctggaag gcttgaagct cgtgttccgc
     4261 accagcagcc cggtcatcat ctatccatca tccggcaccg gggcatggga ggctgcgctc
     4321 gtcaacacac tgtctgctgg cgatcgcgtg ctcatgtccg agaccggcca attcgcaacg
     4381 ctgtggcaaa aacttgcgac gcgcctgggc ctcactatcg attttattcc aggcgattgg
     4441 cgccatggcg cgaatgctga aacgatcgaa cacaagcttg ccgaagaccg tcagcacgcc
     4501 atcaaagcag tctgcgtggt acataacgaa acctctaccg gcgtggtcac tcgcattccg
     4561 ccggtgcgca aggcgatcga tagcaccggt catcctgcgc tgctgctggt tgataccatt
     4621 tcgtcgctcg ggtcgatcga ttaccgccat gacgaatggg gtgtggacgt taccgtcgca
     4681 ggctcccaaa agggcttgat gctgccaccc gggctcagct tcaatgcaat cagcgccaag
     4741 gcgctggcgg cgtccaagcg ggcgcggctg ccgcgaagct atttcagttg ggatgaaatg
     4801 ctcggtccca atagatccgg cttctttcct tacactccgg ccacgaactt gctgtacgga
     4861 ttgcgtgaag cattgcagat gctgcaggag gaagggctcg agcatgtatt ttcccgtcac
     4921 gcccgccacg ccgaggccac gcgccgtgcg gtacgcgcct ggggactgga agtcctgtgc
     4981 gccgagcccg aggaatacag caacacgctg accgccgtgc tgctgccagc aggtcaggat
     5041 gcggacaagg tgcgtgaact gatccttgag cgcttcgaca tgtcattggg tgccggactt
     5101 ggaaaactgc agggcaaaat atttcgcatc ggccatttgg gtgattttaa cgacctcacc
     5161 ctcgccggta cctt
//
LOCUS       DATMZQ010000142         4717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1146620, whole genome shotgun sequence.
ACCESSION   DATMZQ010000142 DATMZQ010000000
VERSION     DATMZQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146620"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VN496_04680"
     CDS             complement(<1..200)
                     /locus_tag="VN496_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXF16274.1"
                     /translation="MSRIGNLPVSVPKGVDVTVASGQIAIKGPLGTLSQALSETVEVQ
                     RDGDHIVFKSMDESMQTNAMSGT"
     gene            complement(222..617)
                     /gene="rpsH"
                     /locus_tag="VN496_04685"
     CDS             complement(222..617)
                     /gene="rpsH"
                     /locus_tag="VN496_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXF16275.1"
                     /translation="MSMSDPIADMLTRIRNAQTAEKTSVVVPLSKLKISIAKVLKDEG
                     YIEDFIARDDGGKGFLEIALKYYAGRPVIERIERVSRPGLRIYKPANDIPEVMNGLGV
                     AIVSTSRGVMTDRKARQTGVGGEVLCIVA"
     gene            complement(631..936)
                     /gene="rpsN"
                     /locus_tag="VN496_04690"
     CDS             complement(631..936)
                     /gene="rpsN"
                     /locus_tag="VN496_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXF16276.1"
                     /translation="MAKIAVTNRDQKRRATVKKFAAKRAQLFAVINNSKASDEDRMQA
                     REKLGKLPRNASPVRLRNRCALTGRPRGVFSKFGLGRNKLREIAMRGEIPGVTKASW"
     gene            complement(944..1534)
                     /gene="rplE"
                     /locus_tag="VN496_04695"
     CDS             complement(944..1534)
                     /gene="rplE"
                     /locus_tag="VN496_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXF16277.1"
                     /translation="MAKAKVKAAPGQAERGHAARLQTHYRNEVVPALMKQFGYKTSMQ
                     VPRIDKIVLNMGVGEAVNDKKILENAVGDMIKIAGQKPVVTKAKKSIAGFKIRKDYPI
                     GCMVTLRRGRMYEFLDRLVSIAIPRIRDFRGISARSFDGRGNFNLGVKEQIIFPEVEY
                     DKIDALRGMNVTITTTAKSDDEARALLAAFRFPFKN"
     gene            complement(1544..1861)
                     /gene="rplX"
                     /locus_tag="VN496_04700"
     CDS             complement(1544..1861)
                     /gene="rplX"
                     /locus_tag="VN496_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXF16278.1"
                     /translation="MRKIRKGDNVIVIAGKDKGKRGSVLRVLDESFLLVEGANSVKKH
                     QKPNPMKGTTGGIVTKEMPIHVSNVALFNPTTQKADRIGIKRLDDGRRVRIYKSNGEM
                     LDA"
     gene            complement(1871..2239)
                     /gene="rplN"
                     /locus_tag="VN496_04705"
     CDS             complement(1871..2239)
                     /gene="rplN"
                     /locus_tag="VN496_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXF16279.1"
                     /translation="MIQMQSRLDVADNTGARSVMCIKVLGGSKRRYAGIGDVIKVSIK
                     DCAPRGRVKKGEIYNAVVVRTASGVRRQDGSLVKFDSNAAVLLNAKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(2393..2668)
                     /gene="rpsQ"
                     /locus_tag="VN496_04710"
     CDS             complement(2393..2668)
                     /gene="rpsQ"
                     /locus_tag="VN496_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXF16280.1"
                     /translation="MSEQTSTSSPRKLTGRVTSDKMNKTVTVLVERKVKHPLYGKFVS
                     RSKKYHAHDEKNEFHPGDLVLIEETRPMSKTKAWKVVQLVEKAKGEN"
     gene            complement(2665..2859)
                     /gene="rpmC"
                     /locus_tag="VN496_04715"
     CDS             complement(2665..2859)
                     /gene="rpmC"
                     /locus_tag="VN496_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXF16281.1"
                     /translation="MKPSELRTKSEADLGKELNELLKAQFSMRMQLATQQLNNTSEIK
                     KVRRDIARVRTLIGEKAKKA"
     gene            complement(2862..3275)
                     /gene="rplP"
                     /locus_tag="VN496_04720"
     CDS             complement(2862..3275)
                     /gene="rplP"
                     /locus_tag="VN496_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXF16282.1"
                     /translation="MLQPARRKYRKEQKGRNTGVATRGAKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRIFPDKPISQKPAEVRMGNGKGNPEYYVAEIQPGKV
                     LYEMDGVDETLAREAFRLAAAKLPIQTTFVIRQVG"
     gene            complement(3286..3969)
                     /gene="rpsC"
                     /locus_tag="VN496_04725"
                     /pseudo
     CDS             complement(3286..3969)
                     /gene="rpsC"
                     /locus_tag="VN496_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(3972..4307)
                     /gene="rplV"
                     /locus_tag="VN496_04730"
     CDS             complement(3972..4307)
                     /gene="rplV"
                     /locus_tag="VN496_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXF16283.1"
                     /translation="MRTSAVLRGVRLSAQKGRLVADQIRGMPVDKALNLLAFSPKKGA
                     RIIRKVLESAIANAEHNDGADIDELQVRIIHVEQGTSLKRFMPRAKGRGFKITKPTCH
                     IYLTVGDKE"
     gene            complement(4329..4628)
                     /gene="rpsS"
                     /locus_tag="VN496_04735"
     CDS             complement(4329..4628)
                     /gene="rpsS"
                     /locus_tag="VN496_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXF16284.1"
                     /translation="MARSIKKGPFVDHHLVQKVETARASKDKRPIKTWSRRSTILPDF
                     VGLTIAIHNGRQHVPIYISENMVGHKLGEFALTRTFKGHTGDKKVVVAPTSPPKK"
BASE COUNT          860 a         1420 c         1251 g         1186 t
ORIGIN      
        1 gtgccggaca ttgcgttggt ctgcattgac tcgtccatcg atttaaagac gatgtggtcc
       61 ccatcgcgct ggacctcaac ggtctccgaa agcgcttgcg acagcgtgcc caggggaccc
      121 ttgattgcga tctgaccgga agctacggtt acatctactc ccttgggaac cgaaaccgga
      181 agattgccaa tacgagacat ctttgtctgc tcctaaccag gttatgcaac gatgcacaac
      241 acttcgccgc ccacgccagt ctgacgggcc ttgcggtccg tcatgacgcc gcgcgaggtc
      301 gatacgatcg ccacaccaag cccgttcatg acttccggaa tatcattggc tggcttgtag
      361 atcctcaggc cagggcgact cacgcgctcg atacgctcga taactggccg gccggcgtaa
      421 tatttcagtg cgatctcgag aaagcccttg ccgccgtcat cgcgagcgat gaaatcctcg
      481 atatagcctt catccttgag aaccttggcg attgaaatct tcagtttgct caggggcacg
      541 acgactgatg tcttttccgc agtttgagcg ttgcggattc gcgtcagcat gtcggcaatg
      601 gggtcactca tgctcatcca gagtctcctg ctaccagcta gctttggtca cgccggggat
      661 ctcgccacgc atggcgattt cacgcagctt attgcggccg aggccgaact tgctaaacac
      721 gccgcgcgga cggccggtca gcgcacagcg attgcgcagc cgaaccgggc tggcattgcg
      781 cggcagcttg ccgagttttt cgcgcgcctg catgcgatct tcatccgacg ccttgctgtt
      841 gttgatgact gcgaacagtt gcgcacgttt tgccgcaaat ttcttgacgg ttgcccgccg
      901 cttttgatcc ctgttggtca cagctatctt tgccataacg acctcaattc ttgaatggga
      961 acctgaaggc ggcaagaagt gcacgcgcct cgtcatcgga cttcgctgtt gtggtgatgg
     1021 tgacgttcat accccgcaac gcgtcgatct tgtcgtactc gacttcagga aatatgatct
     1081 gctcttttac gccgaggtta aaatttccgc ggccgtcgaa tgaacgcgcc gaaatacccc
     1141 ggaaatcccg tatccgcggt attgcaatgg agaccagacg atccaggaat tcatacatgc
     1201 gcccgcgccg tagcgtcacc atgcagccta tgggatagtc cttgcggatt ttgaaacccg
     1261 cgatcgattt tttggccttg gtgactaccg gcttttgccc ggcaatctta atcatgtcgc
     1321 caacggcgtt ctccaggatt ttcttatcgt tgacggcttc acctacgccc atgttcagaa
     1381 ctatcttgtc gatgcgcggg acctgcatgg aagtcttgta gccgaactgt ttcatcaacg
     1441 ccggcactac ctcatttcga tagtgagtct gcagtcgggc cgcatgcccg cgttcggctt
     1501 gtccgggagc tgctttcacc tttgccttcg ccatatcttc cccttacgcg tccagcatct
     1561 cgccgttcga cttgtagatc cgcacacggc ggccgtcgtc cagcctcttt attccgatcc
     1621 ggtccgcctt ctgggtggtg ggattgaaca acgccacgtt ggagacatgg attggcatct
     1681 ctttggttac tatgccgccc gtagttccct tcatcggatt cggcttctgg tgcttcttga
     1741 cgctgttggc gccttccacc aacaggaagc tctcatccag cacgcgcagc acggaaccgc
     1801 gtttaccctt gtctttgccg gcgatgacaa tgacgttgtc gcctttgcga atcttgcgca
     1861 tgatttgtcc ctacaacact tccggagcaa gcgatacgat tttcatgaag cgctcggtgc
     1921 gcaattcacg cgtaacgggc ccgaaaatac gtgtaccgat gggttccagc tttgcattca
     1981 gcaagacggc cgcgttggaa tcgaacttca ccagagagcc atcctgacgg cgcacgccac
     2041 tggccgtccg tacgaccacg gcgttgtaaa tctcgccttt tttcacgcgc ccgcgtgggg
     2101 cgcagtcctt gatgctgacc ttgatgacgt cgccaatccc ggcatagcga cgcttggagc
     2161 cgccaagcac cttgatgcac attacggagc gcgcaccggt gttatcggcc acgtcgagcc
     2221 tgctttgcat ttgaatcatc tgctatctct ctccaacttc gcccgcctct tgaaactagc
     2281 tgggtcggca gaccagtttt ggaacccgtc cgggtcagat cggagcgccc agggggcgtt
     2341 ccggggaaaa aggcggcatt ctatggataa acggccacca cggcaagggg tatcagtttt
     2401 cgcccttggc tttctcaacc agctgcacga ccttccatgc ttttgttttg gacattggcc
     2461 gcgtctcttc aatcagaacc aggtcgccag gatgaaattc gttcttttca tcgtgcgcgt
     2521 gatacttttt tgagcgactg acaaacttgc catacagcgg gtgcttgacc ttccgctcaa
     2581 ccaacaccgt gaccgtcttg ttcatcttgt cgctggttac ccggccggtc aatttccggg
     2641 gactggaagt cgaggtctgc tcgctcatgc ttttttcgcc ttttcaccaa tcaacgttcg
     2701 cacgcgagca atatctcggc gcactttctt tatttcgcta gtgttgttca gttgctgcgt
     2761 agcgagctgc atgcgcatgg aaaactgggc tttcagcagc tcattgagtt ccttgcccag
     2821 gtcagcttcg gacttggtgc gtagttcgct tggcttcatc cttatcccac ctgtctgatg
     2881 acgaacgtag tctggatcgg cagtttcgcg gccgccaggc gaaatgcctc gcgggccagc
     2941 gtttcatcca ccccgtccat ttcatagagc accttgccgg gctggatttc ggcaacgtag
     3001 tactcgggat tgcctttgcc gttgcccatg cgcacttccg caggtttctg cgaaataggc
     3061 ttgtccggaa aaatgcgaat ccagatccgc ccaccgcgct tgatatggcg cgtcattgca
     3121 cgccgggcgg cttcgatctg gcgcgccgtc aatcggccgc gaccaactgc cttcaacccg
     3181 aactcgccga agctgacctt ggcaccgcgc gttgccacgc cagtgttgcg gcccttctgc
     3241 tccttgcgat atttccttct agccggttgc agcattcttt gctcctcgtc ttgccttgcg
     3301 ctccggttcc ggggcagggg cagccaccgg ctgttcccca cgacccagca cttcgccctt
     3361 gaacacccag accttgatgc cgatcacgcc gtaggtcgtc ttggcctcgg aaaatccata
     3421 gtcaatgtcg gcgcgcaggg tgtgaagcgg aacgcggcct tcccgatacc actcggtccg
     3481 ggcgatctcg atgccgttca gtcgcccggc gctcatgatc ttgattccct gcgcgccaag
     3541 gcgcatcgca ttctggatcg cccgcttcat ggcacgccga aacatgatcc gtttttcgag
     3601 ctgctgcgcg atcgaatcgg cgatcaactg cgcatccacc tcgggtttgc ggacttcctc
     3661 gatgttgacg tgcaccggca cacccatgag tttttgcagc tgaccgcgca gattctcaat
     3721 gtcctcgcct ttcttgccga tgacgacgcc gggacgcgca ctgtaaacgg taatcttggc
     3781 gtttttcgcc ggacgctcga tgatcacttt gctgaccgaa gcgtgcgcga gcttcttctt
     3841 caggaagtca cgcaccttga tgtcttcctg cagcattgcc ggaaacgttt tgctattcga
     3901 gtaccagcgc gatgtccaat tacgctgcac ggaaagccgg aatcccgtcg ggtgaatttt
     3961 ctgtcccatg cttactcctt gtccccgacg gttagataaa tatggcaggt aggcttggtg
     4021 atcttgaaac cacgcccttt cgcgcgaggc atgaagcgct tgagactcgt gccctgttcc
     4081 acgtggataa tcctcacttg caattcatcg atatcggcgc cgtcgttatg ctcggcattg
     4141 gcgatagccg attccagcac cttgcgaatg attctggccc ctttcttggg gctgaatgcc
     4201 agcaggttga gcgccttgtc gaccggcatt ccacgtatct ggtccgcgac caagcggccc
     4261 ttctgggccg acagccggac accgcgcaat acagcagaag ttctcatagt catgtctcct
     4321 ggttcccctt acttcttcgg cggcgaagtc ggggccacaa cgaccttttt gtcgccggta
     4381 tgtcccttga aagtacgggt tagcgcaaat tcgccaagct tgtgtcccac catgttttcg
     4441 ctgatataga ttggcacatg ctgccgaccg ttgtgaatcg cgatcgtgag accgacgaag
     4501 tcgggaagga tggtggagcg gcgggaccag gtcttgatcg ggcgcttgtc cttggatgca
     4561 cgagccgtct ctaccttctg caccagatgg tgatcgacga acgggccttt ctttatggaa
     4621 cgtgccatat gtccttacct ttatttgccg tggcggttac gcacgatcat gctgcgtgta
     4681 cgcttgttgc ggcgggtctt gtaacccttt gttggcg
//
LOCUS       DATMZQ010000143         4759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1148940, whole genome shotgun sequence.
ACCESSION   DATMZQ010000143 DATMZQ010000000
VERSION     DATMZQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148940"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VN496_04740"
     CDS             complement(<1..398)
                     /locus_tag="VN496_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HXF16285.1"
                     /translation="MADSLQTIEIETRPNPSHSVIWLHGLGADGNDFVPIVQELALPA
                     IGIRFVFPHAPMRAVTINGGFVMRAWYDIVSQDIARKEDELGLRQSQTMVEELIAREA
                     ARGVPANRVVIAGFSQGGVIALQTALRHPRR"
     gene            complement(413..1003)
                     /gene="mog"
                     /locus_tag="VN496_04745"
     CDS             complement(413..1003)
                     /gene="mog"
                     /locus_tag="VN496_04745"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HXF16286.1"
                     /translation="MENDQVVIGLVSISDRASKGVYKDEGLPALDEWLGKAIRNSRRV
                     IERLIPDEHPVIAHTLKELCDKEGCHLVLTTGGTGPAPRDVTPEATMAVADREMPGFG
                     EQMRQISLHFVPTAILSRQVGVIRGKTLIINLPGQPKSIRETLEGLKSADGKQIVHGI
                     FAAVPYCIDLIGGPYIETDDTVCKAFRPKSAIRPKA"
     gene            complement(1056..1580)
                     /gene="yjgA"
                     /locus_tag="VN496_04750"
     CDS             complement(1056..1580)
                     /gene="yjgA"
                     /locus_tag="VN496_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HXF16287.1"
                     /translation="MPERHVEQFSDDEIRPSRTQRKLNDQALQELGQALVAVGEDKLA
                     ELHLPERLREAILQARTISKFGALRRQMQYIGRLMREEGDANSIRDQLNAWKGASVQE
                     TARLHLIERWRVKLLKDDRALEELIAEYPQADVQRIRMLIRNTRRESEAQKPPKSFRE
                     LFQVLREILATKPT"
     gene            1725..3065
                     /gene="pmbA"
                     /locus_tag="VN496_04755"
     CDS             1725..3065
                     /gene="pmbA"
                     /locus_tag="VN496_04755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HXF16288.1"
                     /translation="MASSEFTHSADTLKQIARDVLDYARERGATAAETEASEGFGQSV
                     TVRLGEVETIEYNRDKGVGVSVYIGNQRGHASTSDFSAQALRDTVDAAISIARLTASD
                     QFAGLADADLLATRFPQLDLYHPWNIDVEQAIELARSCEDAAFAADRNVSNSEGASVS
                     VQQSHFAYGNSNGFLAGYPSSRHSIVCAVIAGKESAMQRDDWYTTARSAIDLEPATEV
                     GARAGKRAARRVGARKVSTARVPVLFEAPVASGLLGHFVAAVSGGSLYRKSSFLLDSL
                     GKPVFAPSVQIHDRPHVLKGLASSPFDDEGVATHTRDIVQGGVLKGYFLGSYSARKLG
                     MRSTGNAGGNHNLSMDSTDGDLASLLKSMGSGLLVTDLLGQGVNMVTGDYSRGAAGFW
                     IENGEISHPVQEITVAGNLKDMYRNIAAIGSDVVVRGSRQCGSVLIEGMTVAGE"
     gene            3241..3852
                     /locus_tag="VN496_04760"
     CDS             3241..3852
                     /locus_tag="VN496_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXF16289.1"
                     /translation="MIALRPLHVLWLLLVLAACSAQPVQLPHLGPDDVVLAFGDSITY
                     GTGAGPEQSYPNVLAQLIHRRVINAGVPGETTADGMARLPSVLEEVRPKLMLLCIGGN
                     DMLRKLDRLTVESNLRAMVQLARERGVDVVLIGVPEPVMFGDTAPVYGNVAKDYHLAF
                     EDRILREVLFDKRLKSDEIHPNASGYRRLAQAIADLLHGTGAI"
     gene            3875..4750
                     /locus_tag="VN496_04765"
     CDS             3875..4750
                     /locus_tag="VN496_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HXF16290.1"
                     /translation="MTTHGKSWFGLGGVPAHDNALAHLWEKRLHWPMIGVALLSIPAL
                     LLEDLTQMPRLHLVGKLLEVVIFLAFAGEFLWMMRLTSHRSEYAIGNWLDLLIILSSA
                     LDLAGWEFEWVAIARLLRLALVGLLLTRIVGSLRNMFSPSGIPFLIGFGLITLGLAGA
                     GFYWIEPTVHSYADGVWLAFVTGSTVGYGDVVPTTTASRLFAGLMVLLGFTLFSLVTA
                     SIAAFFVGEDEKLLRKAMHDDIRKLHDEIAQLIGDEERSLRREMHTDIRQLRREVAEI
                     RQSLGESARDNGPSR"
BASE COUNT          908 a         1411 c         1430 g         1010 t
ORIGIN      
        1 cggcgcgggt ggcgcaaggc ggtttgcaat gcgattacgc cgccttgtga gaaaccagcg
       61 atgacgaccc ggttcgccgg cacgccgcgt gccgcttccc tggcgatcag ttcctcgacc
      121 atggtttgcg actggcgcaa gccaagctca tcttccttcc tggcgatatc ctgcgacacg
      181 atgtcgtacc aggcgcgcat cacaaaccca ccattgatgg tcacggcccg catgggcgcg
      241 tggggaaaga caaagcgaat cccaatggcc ggcagcgcaa gttcctggac gatcggcacg
      301 aagtcgttgc cgtcggcgcc gagaccatgc agccagataa cactgtggct cgggtttggg
      361 cgtgtttcga tttctatcgt ttgcagcgag tcagccatgg ttcgagtttg tatcaagcct
      421 tgggccggat ggccgacttg ggacggaaag ccttgcacac cgtgtcgtct gtttcaatgt
      481 acggtccccc gatcaaatcg atgcaataag gtaccgcagc aaagatgccg tgtacgattt
      541 gcttgccgtc ggcgctcttc agaccttcca gtgtttcgcg gatggacttg ggctgcccgg
      601 gtaaattaat gatcaacgtc ttgccgcgaa taacgccaac ctggcgcgac agaattgcgg
      661 tcgggacgaa atgcaggctg atctgccgca tctgctcgcc aaagccgggc atctcgcgat
      721 cggcgaccgc catcgttgcc tccggcgtga catcgcgtgg cgccggaccc gtgcctccag
      781 tcgtcagcac caggtgacag ccttccttgt cgcacaattc tttcagcgta tgcgcgatta
      841 ccgggtgttc atccggtata aggcgttcaa ttacgcggcg tgaattgcga atggctttgc
      901 cgagccactc atcgagcgca ggcaggcctt cgtccttata gacgcctttg gacgcccgat
      961 cgctgatgga caccagaccg atgacaacct gatcgttttc cataatcaag aagttgaaat
     1021 gcctggagcc cgggtgcccc gcgcgctagg aatgatcaag ttggtttggt cgccaaaatt
     1081 tcccgcaaga cctggaacag ttcgcgaaag cttttcggcg gcttctgcgc ctcgctttca
     1141 cgccgggtat tgcgaatcag catgcggatt cgctgcacgt cggcctgcgg atattcggcg
     1201 atcagctcct cgagcgcccg gtcgtctttc aacagcttga cgcgccagcg ctcgatcaga
     1261 tgcagacggg ccgtttcctg gaccgaggcg cctttccatg cgttaagctg atcgcggatg
     1321 ctgttcgcgt ccccttcttc ccgcatcagg cgcccgatat attgcatctg gcgccgtagc
     1381 gcaccgaact tggaaatcgt gcgcgcctgc aggatcgcct cgcgcagtcg ctcgggcaag
     1441 tgcagttcgg ccagcttgtc ctcgccgaca gcgaccagtg cctgtcccag ctcctgcaag
     1501 gcctgatcat tgagcttgcg ctgcgtccgg cttggccgga tttcatcatc ggaaaattgt
     1561 tccacgtgtc gttcaggcat tgccgccatt gttcccacca tcgcgggcag cgccgcagaa
     1621 tgctgtttgc aagctggtat catagcgaac attccatccc gcggcgtgcc acccgatatg
     1681 tcaggcagca cgccgcttca attttgaagc ttcggaaaga gaccttggca agcagcgagt
     1741 tcacccattc agccgatacc ttgaaacaga ttgcccgcga cgtgcttgac tatgcccgtg
     1801 agcgtggcgc aacggccgct gaaacggaag caagtgaggg attcggccag agcgttacgg
     1861 tgcgcctcgg agaagttgaa acgatcgaat acaaccgcga caaaggagtc ggagtcagcg
     1921 tctatatcgg caatcagcgc gggcacgcca gtacgtcgga cttttctgcg caggcattgc
     1981 gtgacactgt ggatgccgcg atctcaatcg cgcgcctgac cgcatcggac caatttgccg
     2041 ggctggccga tgccgacctg ctggcgaccc gctttccgca gctggatctc tatcatccct
     2101 ggaacatcga cgttgagcag gccatcgagc tggcgcgctc ttgcgaagac gccgctttcg
     2161 cggccgatcg caatgtgtct aattcagaag gcgcatcggt gtcggtgcag caatcgcact
     2221 ttgcttacgg aaactccaat ggcttcctgg ccggctatcc gagctcacgc catagcattg
     2281 tgtgcgccgt catcgccggg aaggaaagtg ccatgcagcg cgacgattgg tatacgaccg
     2341 cccgcagcgc aatcgatctc gaaccggcga cagaagtagg cgcgcgggcc ggcaagcgcg
     2401 ctgcgcgtcg ggttggcgcg cgcaaagtat ccactgcgcg cgtgccggta ctattcgagg
     2461 cgccagttgc atccggcctg ctcggccatt tcgtggctgc ggtcagcggc ggcagcctgt
     2521 accggaagtc gtctttcctg ctcgacagtc tcggcaaacc cgtattcgct ccgagcgtgc
     2581 agatccacga ccggccgcac gtactcaaag gtctggccag cagcccgttc gatgatgaag
     2641 gcgttgcaac gcatacgcgc gacatcgtgc agggcggggt gctgaaaggc tattttctgg
     2701 gtagttattc cgcgcgcaag ctcggcatgc gctccaccgg aaatgccggt ggcaatcaca
     2761 atctcagcat ggattctacc gatggcgacc tggcgtcttt actgaagagc atgggcagcg
     2821 gactgctggt caccgatttg ctcggtcagg gcgtcaacat ggttactggc gactactcgc
     2881 gcggcgccgc tggtttctgg atcgagaacg gcgagatatc gcatccggtc caggaaatca
     2941 ccgtggcggg taacctcaag gacatgtatc gcaacattgc cgccatcggt tccgacgtgg
     3001 tggtgcgtgg ctcacgccag tgcggctcgg tgctgatcga agggatgacg gtggcggggg
     3061 agtgaggccg taaaagccgt aaaggccgtg aaggccgtaa aggtcgtgaa tatcgtgaat
     3121 gtcgcaaaag cagcaggccc tacgccgcta agacattagc aagttcatga gggttttttt
     3181 gcacttacgg attcacgaca ttcacgatat tcacggactc tctcggcttc acaattcacc
     3241 gtgatcgcat tgcgcccctt gcatgtactc tggctgctgc tcgttcttgc cgcctgcagt
     3301 gcccaacctg tccagcttcc tcaccttggc cccgacgatg tggtgctcgc ttttggagac
     3361 agcattacct atggcacggg ggccgggccc gaacaatctt atccgaacgt tcttgcgcaa
     3421 ctgatccatc gtcgcgtcat caatgccggc gtgccggggg aaacgactgc ggatggcatg
     3481 gcgcgtttgc cttccgtcct tgaagaagtc aggcccaaac tcatgctgtt atgcatcggt
     3541 ggcaacgaca tgctgcgcaa gctggatcgc ctaacggtcg agtcaaactt gcgcgcaatg
     3601 gtgcaacttg cccgggaacg tggcgtcgac gtggtgttga ttggcgtgcc ggagccggtg
     3661 atgttcggcg atactgcgcc agtctatggc aacgtcgcga aggactatca tctggctttc
     3721 gaagaccgga tcctccggga agtgctgttc gacaagcgac tcaaatcgga tgagatccac
     3781 ccgaacgcca gcggctaccg gcgtctggcg caggcgattg ccgatctatt gcacggaacc
     3841 ggagccatct agcggcaatg cgcgggggca tggcatgacg acacacggaa aatcctggtt
     3901 cggacttggc ggtgtgccgg cccacgacaa cgcactcgcg cacctgtggg aaaagcgcct
     3961 gcattggccc atgatcggcg tcgcgctgtt atctataccg gcgctgttgc tcgaagacct
     4021 tacccagatg cccaggctac acctggtggg caaacttctg gaagttgtca tctttcttgc
     4081 gttcgccggc gaattcctgt ggatgatgcg gctgacatcc caccgctccg aatatgccat
     4141 tgggaattgg ctggacttgc tgatcatcct gtcctcagcg ctcgacctgg ccggctggga
     4201 attcgagtgg gtggcaattg cgcgcctgct gcggctggca ctggtcggac tgctgctgac
     4261 gcgtatcgtc ggatcgctgc ggaacatgtt ctcgccgagc ggcattccgt ttctcatcgg
     4321 tttcggcctg atcacgctcg gcctcgccgg agccggtttt tactggatcg aaccgacggt
     4381 gcacagctac gccgacggcg tgtggctggc gttcgtgacc gggtcgacgg tcggctatgg
     4441 agacgtcgtg cccaccacga ctgcctccag gctatttgcc ggcctcatgg tgttgctggg
     4501 ctttacgttg ttctctctcg tgacggcgag cattgccgca tttttcgtgg gcgaggacga
     4561 aaaactgctg cgcaaggcga tgcacgacga catccgcaag ctgcatgacg aaatcgctca
     4621 attgatcggc gatgaggaac gctcgctgcg ccgggaaatg cacaccgaca tccggcaatt
     4681 gcgcagggaa gtggctgaaa tcaggcaatc actgggcgag tccgcgcgcg acaacggccc
     4741 atcgcgatga ccctggatt
//
LOCUS       DATMZQ010000144         5752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2876842, whole genome shotgun sequence.
ACCESSION   DATMZQ010000144 DATMZQ010000000
VERSION     DATMZQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876842"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VN496_04770"
     CDS             <1..1364
                     /locus_tag="VN496_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXF16291.1"
                     /translation="INPMNLTEELRHYVVDSDARVAIIGQELLAQVRPLIGEGLQQVI
                     VAAYSDYRTHVTDLKIPDVVAAPRQPLREAGVTGWHDALGENLVPSAHAAGSYDLCVM
                     PYTSGTTGKPKGCIHTHRNVMSTTVAGGAWVDMQSETVILGSMPLFHVTGMQCSMNQP
                     IYFGATIVLMTRWDRDTAGELIQRHKIEGWTNIATMAIDFFSNPNLGRYDISSMKHIG
                     GGGAAMPEAVAQRLLELTGLNYIEGYGLSETIAPTHINPPQRAKKQCLGIPICATESL
                     VINPDTLAVLGPRETGEIVSRGPQIFRGYWKQPEATAAAFVDIGGKSFFRTGDLGYRD
                     EEGYFFIVDRLKRMINASGYKIWPAEVEALLYHHADVREACVIGTQDARRGETVKAVV
                     VLKDTSRGKVTSDDIISWARENMAAYKVPRVVEFIDSLPKSATGKVQWRLLQEKENKN
                     LSR"
     gene            1373..3058
                     /locus_tag="VN496_04775"
     CDS             1373..3058
                     /locus_tag="VN496_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXF16292.1"
                     /translation="MEQKHFAYWPKHLPRHLTVPATSLHFNLEVSSQRYPDKPAIIFY
                     DSKLSYRELHRQVELMAGFLQNRCGVRKGDRVVLQMQNSPQFIIAYYAILRADAAVLP
                     VSPMHVTDELLHYFEDSDAATVLVAQDLYAQIRPLLGKQAKHAIVATYSDFLTEQTTL
                     DVPELLRAPRHVMADPGAVSWSDAIAANYEPLEPAAQPDDLAAILYTSGTTGKSKGCM
                     HTHRTIMHTLVGAVLWEGINADSVALSTAPLFHVTGMQHSMNAPIYAGATIAVLPRWN
                     ADAAGYMIERYGCTHWANVPTMVVDLLAHPSTAGRNLSSLSNIFGGGASMPEAVAQKL
                     FELCGIEYMEAYGMTETISQTHMNPAHKARKQCLGFPTFDTESRIINPETLAVLGPNE
                     QGEIVVHGPQVMQGYWKRPEANEAVFIELEGKKFMRTGDLGRCDEDGYYYIADRLKRM
                     INASGFKVWPAEVEATLYKHPDIREVAVISAPDARRGETVKAVVVVKEERLGQVTAAD
                     IIEWSRLHMAAYKVPRIVEFTDALPRSGTGKIQWRSLQEKEWEDPQGAVARNV"
     gene            complement(3075..3341)
                     /locus_tag="VN496_04780"
     CDS             complement(3075..3341)
                     /locus_tag="VN496_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXF16293.1"
                     /translation="MNTILAGAIGAAFITASLFFLRFWKSTRDRFFLFFALSFFIEGI
                     NRIVLYASIGDNEQSPIYYLIRLVTYGLILAAIIDKNRSGRPRR"
     gene            complement(3349..3606)
                     /locus_tag="VN496_04785"
     CDS             complement(3349..3606)
                     /locus_tag="VN496_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXF16294.1"
                     /translation="MAPLIYTLCMLTSLACAWMLLRGYIVAHHRLLLWSGLCFVGLTL
                     SNFILVLDVVVFPEIDLSLLRMAVALVAISLLLFGLIWEDR"
     gene            complement(3609..3980)
                     /locus_tag="VN496_04790"
     CDS             complement(3609..3980)
                     /locus_tag="VN496_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16295.1"
                     /translation="MPIDEDLVRFIHTSIPSVPYLEALLLLRSEPANPWKAELIAHRL
                     YTSVTGVKELLAELCAAGLAINIDGTYRYNPQSAELQQLIDRLAQSYSSHLLEVTRII
                     HSGTGKKAQIFADAFKWRKEP"
     gene            complement(4084..5442)
                     /locus_tag="VN496_04795"
     CDS             complement(4084..5442)
                     /locus_tag="VN496_04795"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXF16296.1"
                     /translation="MKKHMRAILAALVFVLPGLFGSGPATAGAVPLAQAGVPTLAPLV
                     ERVTPAVVNIAVLSRSPEHDNPMLQDPFFGRLFGPPERSQPQISAGSGVIVNARLGHV
                     LTNQHVVKDATQIVITLKNGRQLEAKLLGADIATDIALLEVEPDNLNAIRFADSDQLR
                     VGDYVLAIGNPFGLGQTVTSGIVSALGRTGLNVEGYEDFIQTDASINPGNSGGALVNL
                     KGELVGLNTAIIGPAGANVGIGFAVPSNMARAVMAQLAKYGEVRRGRIGLTTQDVSPE
                     VPNAPGSRPGHGAVVVEVAPDSPAAKSGLKRGDVVLSVNGRPVRSSADLRNQIGLAAV
                     GDLQEFKVLREGKELMFRAKVESSKPATTRGLEMVPEMNGATVGTLETGGEPEAVAVL
                     DVAQGSSAYQRGLRAGDVIVAVNRGKVRTAAELATILKRPGRVVLSVVRGDYVFGLVL
                     RD"
BASE COUNT         1308 a         1696 c         1616 g         1132 t
ORIGIN      
        1 cgatcaatcc catgaatctg accgaggagc tgcgccacta tgtggtcgac agcgacgcgc
       61 gcgtggcaat catcgggcag gaattgctag cccaggtacg tcccctgatc ggcgagggcc
      121 tgcagcaggt tatcgtggcg gcatattccg attaccgcac gcatgtcacc gatctcaaaa
      181 tcccggacgt ggtcgccgcg ccgcgccagc cgttgcgcga ggcgggcgtg acgggttggc
      241 acgacgcgct cggcgaaaat ctggtgccgt cggcgcacgc ggcaggctct tatgatctat
      301 gcgtcatgcc ttatacctcc ggcacgacgg gaaaacccaa gggctgcatc cacacgcatc
      361 gcaacgtcat gagtacgact gttgcgggcg gggcctgggt ggacatgcag tccgagacag
      421 tcatcctggg ttccatgccg ctgttccacg tgactggcat gcaatgcagc atgaaccaac
      481 ccatctattt cggcgccacg atcgtgttga tgacccgctg ggatcgcgac acggcgggcg
      541 aactgatcca gcgccacaaa atcgaaggct ggaccaacat cgcaaccatg gcgatcgatt
      601 ttttttcgaa cccgaatctg ggtagatatg acatttccag catgaaacac attggcggcg
      661 gaggcgccgc catgccggag gccgtggcgc agcggctgct cgaactgacg gggctcaact
      721 acatcgaggg ttacggcctg tcggaaacga ttgctccgac gcatatcaat ccgccacaac
      781 gcgcgaagaa gcaatgcctg ggtatcccga tctgcgccac ggagtcgctg gtcatcaatc
      841 ccgatacgtt ggccgtgctt ggcccacgcg agaccggcga aatcgtttcg cgcgggccgc
      901 agattttccg cggctactgg aaacagcctg aagcgaccgc ggcagcattc gtcgacatcg
      961 gcggcaagtc atttttccgt actggagatc ttggctatcg ggacgaggaa ggatatttct
     1021 tcatcgttga ccgtctgaag cgcatgatta atgcgtccgg ctacaaaatc tggccagccg
     1081 aagtcgaagc cctgctctat catcacgccg acgtacgtga agcatgcgta atcggcacac
     1141 aggatgcgcg gcggggtgaa accgtgaagg cagtggttgt gctgaaagac acgagccgcg
     1201 gcaaagtgac atccgatgac attatctcct gggcgcgcga aaacatggcc gcctacaagg
     1261 taccgcgcgt ggtcgaattc atcgactccc tgccgaagtc agccaccggc aaggtacagt
     1321 ggcgcttgct gcaggaaaaa gaaaacaaaa acctttcacg ctgaaaccat caatggaaca
     1381 gaagcatttc gcatactggc ctaagcacct gccacggcat cttacggtgc cggccacctc
     1441 actgcatttc aacctcgaag tttcgagcca acgctatccc gacaagccgg cgattatttt
     1501 ctacgacagc aaactgtcgt atcgcgaact gcaccgtcag gttgagctca tggccggttt
     1561 ccttcagaac cgttgcggtg tgcgcaaggg cgatcgcgtc gtgctgcaga tgcaaaattc
     1621 accgcagttc atcattgcct actacgccat cctgcgcgcg gatgctgcgg tattgccggt
     1681 gagtcccatg cacgtcacgg acgagttgct gcactacttt gaagattccg acgccgctac
     1741 ggtgctggta gcccaggatc tgtatgcgca gatccggccc ctgctcggca agcaggccaa
     1801 gcacgccatc gtcgcgacct attccgattt tctaaccgaa cagacgaccc ttgacgttcc
     1861 cgagctcttg cgcgcgccac gccatgtgat ggcagacccc ggggcggtat cctggtcgga
     1921 tgctatcgcg gccaactacg aacctcttga gccagccgcg cagccggacg atcttgcggc
     1981 gatcctctac acctccggga cgaccggcaa gtccaagggt tgcatgcata cccatcgcac
     2041 catcatgcat acactcgtcg gagccgtcct ctgggagggt atcaatgccg attcggtcgc
     2101 gttgtccact gcacctttgt ttcatgtgac cggcatgcag cacagcatga acgcgccaat
     2161 ttatgctggc gccaccatag ccgtgctgcc gcgctggaat gcggatgcgg cgggatacat
     2221 gattgagcgt tacggctgca cgcattgggc caacgtgccg accatggtgg tcgatctgct
     2281 ggctcatccg agcactgccg gacgcaatct gtcgtcgttg tcgaacattt ttggcggtgg
     2341 cgcatccatg ccggaagccg tcgcgcagaa gctatttgaa ctatgcggca ttgaatacat
     2401 ggaagcctac ggaatgacag aaaccatttc gcagacccac atgaatccgg ctcacaaggc
     2461 acgcaaacaa tgcctgggat tcccgacgtt cgacaccgaa tcgcgcatca ttaacccgga
     2521 gacgcttgcc gtgcttgggc ccaatgaaca aggcgaaatc gtcgtgcatg gtccgcaagt
     2581 catgcaggga tactggaagc ggcccgaagc gaacgaggcg gtcttcatcg aactcgaagg
     2641 caagaagttc atgcgcaccg gagacctcgg ccgttgcgac gaagacgggt attactacat
     2701 tgccgatcgc ctcaagcgca tgatcaacgc atctggattc aaggtctggc ctgcagaagt
     2761 cgaagccacg ctctacaagc atccagatat ccgggaagtc gcagtcattt ccgctccgga
     2821 tgcccgtcgc ggcgaaacag tgaaagcagt ggtggtcgtc aaggaagaac gccttggcca
     2881 ggtcactgca gccgacatta ttgaatggtc acgcttgcac atggcggcct acaaggtgcc
     2941 gcggattgtc gagttcaccg acgcccttcc ccgctccggc acgggcaaga tccagtggag
     3001 aagcctgcag gaaaaggagt gggaagaccc gcagggcgcg gtggcacgaa atgtgtagcc
     3061 gctgacgcca gtggttatct acgcgggcga ccggatcggt tcttgtcgat gatcgctgcg
     3121 aggatgaggc cgtaagtgac gagccgtatc aagtaataga tgggcgattg ctcattgtcg
     3181 cctatggaag cgtacaagac gatgcggttt ataccctcta taaaaaaaga cagcgcgaag
     3241 aaaagaaaaa agcggtcgcg cgtgctcttc cagaaacgca ggaaaaacag gcttgccgtt
     3301 atgaacgcgg cgccgattgc gcctgccagg atcgtattca tggcggcctc atctatcttc
     3361 ccagatcaaa ccgaacagca gcagtgaaat cgcaaccaat gcgacggcca ttcgcaacag
     3421 cgacaggtcg atttccggaa aaacgaccac atcaaggacc aggatgaaat tgctcagcgt
     3481 cagtccgaca aagcacagcc cgctccacag aagcaatcta tggtgcgcga cgatatagcc
     3541 gcgcagaagc atccatgcac acgcaagact ggtcaacatg caaagcgtgt aaatcaacgg
     3601 ggccataatc aaggctcctt cctccacttg aacgcatcgg cgaagatctg tgccttcttt
     3661 cccgtaccgg aatggatgat acgggtgact tcaagcaggt gactggaata actttgcgca
     3721 agccggtcta tcaactgttg caattcagcc gactggggat tgtaacgata agtgccgtca
     3781 atgttgattg ccaaaccagc agcgcacaat tcggcgagca gctctttcac acccgtaacc
     3841 gacgtataaa gccggtgcgc aattaattcc gccttccagg gattggccgg ctcgcttctg
     3901 agcaacagta gcgcctccag atatggaaca gacggaatgc tggtatgaat gaaacgtacc
     3961 agatcttcgt caattggcat ttttcgcgtt ttaatcgtca gcgataatgg tgaccgaaaa
     4021 cgatacctaa ttcggtatac ggctgcaatt tcgaaaaatc gtcgtccgca gtactttctg
     4081 ccttcaatcc cgcaatacca ggccgaatac atagtctcct cgtacaaccg aaagcacaac
     4141 tcggcccggc cgtttaagaa tcgtcgcgag ttcagctgca gtgcggacct tgccgcggtt
     4201 cactgcaacg atcacatcgc ccgcacgcag tccgcgttga taggcggaac tgccctgcgc
     4261 cacgtccaac accgcgaccg cctcgggctc gccgccggtt tcaagcgtgc cgactgtggc
     4321 gccattcatc tcgggcacca tttcaagtcc acgggtagta gcaggttttg aactctcaac
     4381 ttttgcccgg aacatcaatt ctttgccttc gcgaagcacc ttgaattcct gaagatcgcc
     4441 gacggcggcc agcccgatct ggttgcgcaa atcagctgag ctgcgtaccg ggcgcccgtt
     4501 gacggacagc accacatcgc cgcgcttgag tccggatttg gctgcaggag agtccggtgc
     4561 gacttccact acgactgcac catggcccgg cctggagcct ggtgcattgg ggacttccgg
     4621 ggacacgtct tgtgtcgtca ggccaatgcg cccgcggcgg acttcgccgt acttggccag
     4681 ttgcgccatc acggcgcgcg ccatgttgct gggcacggca aagccgatgc ctacgttcgc
     4741 tcccgccggg ccgatgatcg cggtattcaa tccgacgagt tcacctttga gattcaccag
     4801 cgctccaccg gaattgccgg gattgatgga agcatcggtc tggatgaaat cttcatagcc
     4861 ctcgacattg agtccagtgc ggcccaacgc actgacaatg cccgacgtta cggtttgtcc
     4921 tagcccgaac ggattgccga ttgccagcac ataatcgccg acacgcaact gatcggagtc
     4981 ggcaaaccgg atcgcattca aattgtcggg ctcgacctcc aacaacgcga tgtccgttgc
     5041 gatatcggcg cccagaagtt tcgcttccaa ctggcgtcca ttctttagcg tgatgacgat
     5101 ctgggttgcg tccttgacga cgtgctgatt ggtgagcaca tggcccagcc tggcgtttac
     5161 gatgacaccc gatcccgccg aaatctgcgg ctgcgaacgt tccggcgggc cgaagagccg
     5221 tccgaaaaac gggtcctgca gcatgggatt atcgtgctcc ggcgagcgcg acagcactgc
     5281 gatgttcacc accgccggcg tgacgcgttc gaccagtggc gccagcgtcg gcactccggc
     5341 ttgcgccagg ggaacggcgc cggcggtcgc cggcccggat ccgaagagac ccggcagcac
     5401 aaaaactaag gcggcaagaa tggcgcgcat atgtttcttc attttcgtta ttggtaacgt
     5461 taaccagggc acgcacaaac aggataagta tgccacccgt gtgctgcaac ccgcccggcg
     5521 ccagagacgg cttctgctat ccttgcatca tcaaagccgc ggagaaagca tctgaattta
     5581 cagaatagac aagaaagtgc attacaagcc gacattctcg ccgcagtagc ggcaaggcag
     5641 gaagcactga ccggactgat atcgtcgctc gtgcgtttcc cgtcgctcaa cggcgaagag
     5701 accggggccc aggatttcat ggaaaactta ttccaggaaa tgggccttgc aa
//
LOCUS       DATMZQ010000145         7798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3512003, whole genome shotgun sequence.
ACCESSION   DATMZQ010000145 DATMZQ010000000
VERSION     DATMZQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512003"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="VN496_04800"
     CDS             complement(<1..1759)
                     /locus_tag="VN496_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXF16297.1"
                     /translation="MARAADNHTPAFVGSETCAGCHQSEAKAWRSSQHERAMAHATDK
                     SVLGDFNDASFENYGVRSRFFRKDGKFLVETDGPDGKLAIFEVKYTFGVDPLQQYLIE
                     FPDGRVQALSIAWDSRPKDKGGQRWIHLYPNEQIKHDDILHWTKLNQNWNFMCAECHS
                     TGIRKNYDAANDRYATTWAEISVGCEACHGQGSNHVAWARTQQSWWPFGKTKDATKGL
                     LVQFDERAGVTWTQNDRTGMPQRNGMPLGPRKEVETCGLCHARRDPFSDKWIPGQPLS
                     NTHLPTPIIRTSSYPDGQMRDIEEIYNYLPFRQSRMFAAGVTCSDCHDPHSAALRAPG
                     DGVCGQCHLASKYETVSHRHHESVSGSIPCASCHMPERKYMIVDRRHDHSFRIPRPDQ
                     SAKLGTPNACNDCHTDKPAQWAASAVETWYGPQRKGFQTYAEAFHSAWTRQADAERLL
                     GQVASDRNVPAVARASALTALSAYLSPANINLAQKGLVDSDPMVRGGALSMLEGVPAN
                     QLWPLVSPLLSDPIAGVRIRAVSLLARVPTASQPGTDRESFDRAEAEFLAAQRLNADR
                     PEARTTLANFLAQRGKSVEA"
     gene            complement(1869..2198)
                     /locus_tag="VN496_04805"
     CDS             complement(1869..2198)
                     /locus_tag="VN496_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16298.1"
                     /translation="MTKLEILAATVAVAVCSSPLTVRADDVPTYDVNKSCKADVQAYQ
                     GTGTRAGCMTDEQNARQMLVSQWTQFAPESRARCSQMVSDPAGPQSYVELLTCLQMAK
                     DVKSLPK"
     gene            complement(2268..3251)
                     /locus_tag="VN496_04810"
     CDS             complement(2268..3251)
                     /locus_tag="VN496_04810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXF16299.1"
                     /translation="MAALLTSAAAIALSKSPALAQGDPLPSWNDGKAKQSILDFVANV
                     TREGSANFVPVPQRIATFDNDGTLWVEQPMYTQLAFALDRVKALAPQHPEWKKKQPFK
                     AVLDGDMRALAGTGEHGMVELVMATHANMSTRDFERVVTDWVSTARHPRFKQPYTDCV
                     YQPMIELLAYLRANGFKTYIVSGGGIEFMRPWTERVYGVPPEQVVGSSIKTRYVLRSD
                     IPILLRLPDVNFVDDGPGKPVGINGHIGRRPIAAFGNSDGDLQMLEWTTKAAATPALA
                     LIVHHTDAEREYAYDRNSHVGKLDKALDAAKLNNWTVVSIKDDWKRIFKFQ"
     gene            3643..4104
                     /locus_tag="VN496_04815"
     CDS             3643..4104
                     /locus_tag="VN496_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16300.1"
                     /translation="MRVKVTFVLAAATAALLSVIYAESEALARGGGGRGGHGSMTSGS
                     HGPSSHGMRHMPRFASKGQPHGKHNMNHHAMGDHGKSANKQGVWHKVTQRNNNFQGQW
                     QVGGGGGGGSVGDMCINAGACQGIWQVAPQPDSQTSQSRSLQRSWHVGNEM"
     gene            complement(4145..4966)
                     /locus_tag="VN496_04820"
     CDS             complement(4145..4966)
                     /locus_tag="VN496_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HXF16301.1"
                     /translation="MESRRSKLRTVLLAVAAALVAYGILAYVVLPMSWTHYEHQKGLA
                     GRPMVTRTAQDIPGGPINVGLVGQKEDVLCSMHAAAWYPADPITFRSSVEIVGSVVLR
                     RPYRDAPVSPLFYDGRREDLAFEKPVGESADRRNHVRFWQVLKEGQEGRPVWLGSATF
                     DRGVGLSHYTGQVTHDIAPDIDAERNEFTDDLKIAKVVEAIYQITGVGPTLVGRNGEG
                     DRYYTDGEITISRLVTGCNAKAVTVVELDNPPIVNLKNEAWRAVTGILQSGAASR"
     gene            complement(4996..5427)
                     /locus_tag="VN496_04825"
     CDS             complement(4996..5427)
                     /locus_tag="VN496_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16302.1"
                     /translation="MPPRRVIVGSALLMCSLSFALAQQQTLVIPDNLFTQAGFAVKYA
                     TTPEKSRMLRSLPPDKLVKRMKDGRTYYVYADAERCNCAYVGTPEAYAAYQNWSNIGP
                     GGGGSGPLSNSMQTIDSDNIGTPYIPADLGNSMNSILDPSF"
     gene            6089..7114
                     /locus_tag="VN496_04830"
     CDS             6089..7114
                     /locus_tag="VN496_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16303.1"
                     /translation="MRERSAMLASVTFAFVLIAGLVLQSTLSADAEDCIAKPNAPAPQ
                     GEHWYYRIDHANNRQCWRLGPEGLRVQSEGPRAQKSAPQIEKQVAPAAAVQPPVPVRS
                     RRPETTGSSVAAAARAEAAPEPNPASASAASPLRFLDTPRMPDLPPSVQLAPQPEFVG
                     RTQTASAIDTVPAEDNSASSTVNDAPAASSVAEPPRQAAARPSAPPTPATDMEIDHTF
                     AFLMIVFAVLAVAGPTLHYAERRRQREETANYESPRWARVVTPNTPAPRDRVPLQPNL
                     AVDRRPAPIPPTPADQTERLAQALQQLVDRLHTQPRPEPSVTATRPAERTDVEMMRGR
                     RPIALRN"
     gene            complement(7123..>7798)
                     /locus_tag="VN496_04835"
     CDS             complement(7123..>7798)
                     /locus_tag="VN496_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXF16304.1"
                     /translation="TIALESTRTIEIDEFVPKSEIDDLYYIRPYYIAPDGKVGQDAFV
                     VIRDVIEKMNMVAIGRVVLTSREHIIAMQPRGKGLMGMLLRYPYEVRDEKEYFDDISN
                     AKISKDMMDLAKHIVETKSGHFEPDKFEDHYEAALKTLVEKKSRGLKIEAPKERAPAK
                     VINLMDALRRSVKEERQPARKQQAKRSKKRIEGQKEMLFSISSKKAGKEEARRAAPAA
                     KRRRAG"
BASE COUNT         1583 a         2370 c         2201 g         1644 t
ORIGIN      
        1 cagcttcgac agatttgccg cgctgcgcca ggaaattggc gagggtcgtt cgcgcttctg
       61 gccggtccgc gttgagacgc tgcgcggcga gaaactccgc ttcagcccgg tcgaaacttt
      121 cacgatcggt gcctggttgg ctcgcagtcg gaaccctcgc aagcagcgat acggccctga
      181 tgcgcacgcc tgcgattgga tccgacagca aaggcgaaac gagtggccat agttgattgg
      241 ccggaacgcc ctccaacatc gaaagggcac cgccgcgcac catgggatcg ctgtccacaa
      301 gccctttctg ggccagattg atattggctg gcgaaagata agccgacaac gccgtcaacg
      361 cactggcccg ggcaacggca ggaacgttgc ggtccgatgc gacctgacca agaagccttt
      421 cagcatctgc ctgccgcgtc catgcagagt ggaaagcctc tgcataggtc tggaaccctt
      481 tccgctgcgg cccgtaccac gtctccacgg cgctcgccgc ccattgggcc ggcttgtcgg
      541 tgtgacagtc gttgcatgcg ttcggagtac ccaacttcgc cgactgatcc ggcctgggaa
      601 tccggaagct gtgatcgtgc cgccggtcga cgatcatgta tttgcgttca ggcatgtggc
      661 aggatgcaca aggtatcgat ccgctcacac tctcatggtg acggtgtgaa acggtttcat
      721 acttgctcgc cagatggcat tgcccacaca caccatctcc aggcgcacgc aacgcggcgc
      781 tgtgcggatc gtgacagtcg ctgcaggtca cgcccgcagc aaacatcctg ctctgccgga
      841 acggcagata gttgtaaatt tcctcgatgt cgcgcatctg cccatcgggg taagacgatg
      901 tgcggataat cggcgtgggc aaatgtgtgt ttgaaagcgg ttgtcccgga atccatttgt
      961 cggaaaacgg atcgcgcctc gcgtggcaca ggccgcaggt ttccacctcc ttgcgcggcc
     1021 ccaacggcat tccattgcgc tgaggcattc cggttctgtc gttctgcgtc catgtgactc
     1081 cggcgcgctc atcaaattga acgagcaatc ctttcgtcgc atccttcgtc ttgccgaacg
     1141 gccaccagct ttgctgggtt cgcgcccagg caacgtgatt ggagccctgc ccgtgacagg
     1201 cttcgcagcc gacactgatc tccgcccagg ttgtcgcata gcgatcgttt gccgcgtcat
     1261 agttcttgcg gatgccggtc gaatggcatt cggcgcacat gaaattccag ttctgattta
     1321 gtttggtcca atgcagaatg tcgtcgtgct tgatttgctc attggggtag agatgaatcc
     1381 agcgttgccc ccctttatcc ttcggtcggg aatcccacgc gatggacaaa gcctggacgc
     1441 gtccgtcagg aaattcgatc agatattgtt gaagcgggtc gacgccgaaa gtgtatttta
     1501 cctcaaagat ggcgagcttc ccgtcgggcc catcggtttc gacaagaaat tttccgtcct
     1561 tgcggaaaaa gcgcgagcgt acgccgtaat tttcaaaact cgcatcgttg aaatcgccca
     1621 gcacggattt atccgttgca tgagccattg cacgctcgtg ctgtgaggaa cgccaggcct
     1681 tggcttcaga ctgatggcag ccggcacagg tttcactgcc gacgaatgcg ggggtgtgat
     1741 tgtctgcggc acgcgccacc ggcgcgtgca aaaattgcca aagacttcca agcaaaaaaa
     1801 ccggtagcgc cgccacgcag ccggcggcca agattccgtt tctgccgcga aacttcacgc
     1861 cgggcggatc acttgggcaa agatttcacg tccttcgcca tttgaaggca ggtgagcaac
     1921 tccacatagc tctgcgggcc ggcggggtcg ctgaccattt gcgagcacct cgctctgctt
     1981 tccggtgcaa attgtgtcca ttggctcacg agcatttgcc gggcattttg ctcatcagtc
     2041 atgcatcccg cgcgagtccc agtaccctgg taagcctgca cgtcggcctt gcagcttttg
     2101 ttgacatcgt atgtcggcac gtcgtcggca cgcacggtaa gcggcgatga gcagaccgcg
     2161 accgccaccg ttgcagccaa tatctcaagc ttggtcatgt tctctctcct gagtacccag
     2221 cgtttattct cgcggcgcgc tgtcgccttc cgggcaggcg agtccgacta ttgaaatttg
     2281 aagatacgct tccagtcatc ctttatcgaa acaacagtcc aattgttaag cttggccgcg
     2341 tcaagggcct tgtcgagctt gccgacatga ctgttgcgat cgtaggcata ttcgcgctcg
     2401 gcatcggtgt gatggacgat cagggcaaga gccggcgtgg cagcagcctt ggttgtccac
     2461 tcgagcatct gaagatcgcc gtccgaattg ccgaatgctg cgatcgggcg tcgccctatg
     2521 tggccgttga tcccgacagg tttgccgggc ccgtcgtcaa caaaattcac atcgggaaga
     2581 cgaagtaata tcggaatgtc gctgcgcagc acgtaccgtg tcttgatgct cgaccccaca
     2641 acctgctccg gcggaacgcc atagacccgc tcggtccacg gacgcatgaa ttcgatgccg
     2701 ccgccggaca ctatataagt cttgaaaccg ttcgctcgca gataggcaag caattcgatc
     2761 atcggctgat acacgcagtc ggtataaggt tgcttgaagc gcggatgccg ggcagtcgaa
     2821 acccaatccg tgacgacgcg ctcgaagtct cgggtgctca tgttggcgtg cgtcgccatg
     2881 accaactcga ccatgccgtg ctcgcccgtt ccggccagag cccgcatgtc gccatccagg
     2941 actgccttga aaggctgctt ctttttccat tccggatgct gaggtgcaag cgccttcacc
     3001 cggtcgagcg cgaacgccag ttgggtgtac atcggctgtt ccacccacag tgtgccgtcg
     3061 ttatcgaagg tcgcaatgcg ctgcggcacg ggcacgaaat tggccgaacc ctcgcgtgtc
     3121 acgttggcga caaagtcgag gatcgattgc ttcgccttgc cgtcattcca tgaaggcagt
     3181 ggatcgcctt gagccagcgc cggagatttg gagagcgcaa tggccgcggc cgaagtcagc
     3241 agtgctgcca agaccgcgcg tcggtgcggg caaaagaagg cgtgcattag cggtctccag
     3301 tctgaaaaat cacctgtgca agtttaaacc ccgcccagac agctgctcat cagtgcctgg
     3361 tcggatccac gcctggcgat attcaatccg gccatatgtg acctcaattg gcctaaaggg
     3421 aacctgacga tctcggctga ccgggatgcc ggcgcccatg atcacggggc gcccgctcgc
     3481 taaaattaag cttgattctt aatccaggtt gcccgaacag cgctgacgct ggcccacaca
     3541 gcgttaactt aactaaccgc ggctttgtga tttgccactg gcggttaatc tgtacgaccg
     3601 taaggcgcat gagctgatcg tgccattact gggacaatcc ctatgcgcgt gaaagtcact
     3661 tttgttcttg cagctgcaac tgcggcgctt ctgagcgtca tttatgcgga gagtgaagcc
     3721 ttggctcgtg gcggcggagg ccgaggcggg cacggcagca tgacgtcagg ttcgcacgga
     3781 cccagctcgc acggcatgcg ccacatgcct cgctttgcgt ccaagggaca gccgcatggc
     3841 aagcacaaca tgaatcacca cgcgatgggc gatcacggca aatctgcgaa caagcaaggc
     3901 gtgtggcaca aggtcaccca gcgaaacaat aattttcaag ggcaatggca ggttggcggc
     3961 ggaggcggag gcggaagcgt cggcgacatg tgcattaacg caggcgcctg ccagggcata
     4021 tggcaagtcg cgccgcaacc cgacagccag acatcccaat cgcgcagcct ccagagaagc
     4081 tggcacgtcg gcaacgaaat gtaggcgatc gctcacgaac gccccggttg cggacactcg
     4141 ggcattacct ggaagccgcg ccggattgca gaatcccggt aaccgccctc cacgcttcat
     4201 ttttcaaatt cacgatcggc gggttgtcca gctccactac cgtcacagcc ttcgcattgc
     4261 agcccgtaac aagacgcgaa atcgtgattt ctccatcggt gtaatagcga tcgccctccc
     4321 cgttccggcc aaccagtgtc gggccgacgc cggtgatctg atagatcgct tcgacaacct
     4381 tcgcgatttt cagatcgtca gtgaactcgt ttcgctcggc gtcgatgtcg ggcgcgatgt
     4441 catgggtcac ctgtccggta tagtggctca aacctacacc ccggtcgaat gttgcggagc
     4501 cgagccagac tggccggccc tcctgccctt ccttgagaac ttgccagaag cgcacatgat
     4561 tgcgtcggtc ggcgctttcg ccaacgggct tttcaaatgc gagatcttcg cgtcgcccgt
     4621 cgtaaaacag agggctcaca ggcgcatcgc ggtaaggcct gcgtaagaca acgctgccga
     4681 cgatctccac gctcgaccgg aatgtaatcg gatccgccgg ataccatgcg gccgcatgca
     4741 tcgagcacaa gacatcctcc ttttgtccaa ccagccccac gttgatcggg ccacccggaa
     4801 tatcctgtgc cgtgcgcgta accattggac ggcccgcgag ccccttctga tgctcgtaat
     4861 gcgtccagct catcgggagc acgacatagg ccaggattcc gtaagcgacg agcgctgccg
     4921 caacggcaag cagaacggtt ctcagttttg aacggcggga ttccatcgag tggtggccgc
     4981 ggattctttg cgtcctcaga aactcggatc aaggatactg ttcatgctgt tgccgaggtc
     5041 ggcaggaata tacggagttc cgatattgtc ggaatcgatc gtctgcatgg aatttgacag
     5101 cggaccactg ccaccgccgc cgggaccaat gttcgaccag ttctggtacg ccgcgtaagc
     5161 ttccggcgtt cccacatagg cacaattgca acgttcagcg tccgcgtaga cgtaatatgt
     5221 cctgccgtcc ttcatccgct tcacaagctt gtccggcgga agcgatctca gcatccggct
     5281 tttctccggc gtcgtcgcat atttcacggc aaaaccggcc tgcgtgaaca gattgtccgg
     5341 aatgacaagg gtctgctgtt gggcgagtgc gaatgaaagg ctgcacataa gcagcgcgga
     5401 accgacgatg acgcggcgtg gcggcatgat cactcctcac acaataactt caaaatgtgg
     5461 ttcagttgtt aggtggcggc ggcattccag ctccgtcaag ccccggtggt gggcccggat
     5521 tttgcaattg gaggggcagc agcccctgcg cattttctga atatggcggc gtaagcgctg
     5581 acggcggtgc gttgccggcc ggattttgaa cgcatcgccc cccgatcccg ctcagcgtct
     5641 gaaggcattg ctgaaagctg gagtagttgc attgcgtgtt gccatcattg tccatgatgg
     5701 cacaccaggg aaaactctga gcacgcgcag gcgcggccgt aaaaatcgtt gcaaccaaaa
     5761 atagcgacgg tgcaatgaca ctccaggcga tgcatctgta agcgaccgca aacattgtct
     5821 cgtgcctcca ctttcaccgc ggcaagtcga tgggcaacat aacggtaaaa caatagccag
     5881 cctattgccc ttacgggcac tgttgagagc aattgggagg acacgcaggc actgttttac
     5941 cgccaatact agatctgtgc atgccgatac ttgccgaatt tgcaacgcat tgaggcccca
     6001 atcacagttt ccaattggca ttacacacct tgtactcaca catcgagtcg catctctttt
     6061 ttctttctat cgagccagga gggttggcat gcgagaacgc agcgcaatgc tcgcatcggt
     6121 cacgttcgct tttgtcttga ttgcagggtt ggtgctgcaa tcgacactta gcgcggacgc
     6181 tgaagattgc atcgccaaac caaacgcacc ggcgccgcaa ggcgaacact ggtactaccg
     6241 gatcgatcac gcaaataacc gtcagtgctg gcgcctcgga ccggaaggac tccgcgtgca
     6301 atcggaagga cctcgcgcgc aaaagagtgc gccacaaatc gagaagcagg tcgcgccggc
     6361 agccgcagta cagccgcccg tgccggtgcg ctcgcgtcgc ccggaaacca caggatcgtc
     6421 cgttgcagct gctgcaaggg cagaggccgc gcccgagccg aatcctgcgt cagcgtcagc
     6481 ggcctcccct ctgcggttcc tcgatacgcc gaggatgcca gaccttccgc cctccgtgca
     6541 gcttgcgcca cagccggagt tcgtcggacg gacacaaacc gccagcgcga tcgacaccgt
     6601 gccggcggag gacaattccg cgtcgtcaac cgtcaatgac gcgccagcgg ccagctccgt
     6661 cgcggagccg ccacggcaag ctgccgcgcg accttcggcg ccgcctacac cggcaacaga
     6721 catggaaatc gatcacacgt tcgcttttct catgatcgtg ttcgcggtgc tcgccgttgc
     6781 cggcccgaca cttcactacg cggaacggcg ccgtcagcgc gaagaaactg ccaactacga
     6841 gtccccgcga tgggcgcgcg tggttacccc gaatacgccc gctccacgcg atcgtgtacc
     6901 tctccaacca aatctcgcgg tcgacagacg cccagcgccg attccaccca cgccggccga
     6961 ccagaccgaa cggctggcgc aggcgttgca acagcttgtg gaccggctgc atacgcaacc
     7021 acggcccgag ccaagcgtta ccgcaacgcg tccggcagag cgcaccgacg tcgaaatgat
     7081 gagaggccgt cgtccgatag cgctacgaaa ttgactgcgg ctttagcctg ctctgcgccg
     7141 cttcgccgcc ggcgccgccc gtctggcttc ttccttgccg gccttcttgc tggaaatgga
     7201 gaagagcatt tccttctgtc cttcgatccg cttctttgag cgtttcgcct gttgcttgcg
     7261 tgctggctgg cgttcttcct tcacactccg ccgcagggca tccatgagat tgatgacctt
     7321 tgcaggcgcg cgttccttcg gtgcctcaat cttgaggccc ctggatttct tttcgacaag
     7381 tgtcttgagc gcggcttcgt agtgatcttc aaacttgtcc ggctcgaagt gcccggattt
     7441 tgtctccaca atgtgctttg ccagatccat catatccttg ctgatcttcg cgttcgaaat
     7501 atcgtcgaaa tattcttttt cgtcgcgaac ctcgtagggg tatcgcaaca acattcccat
     7561 caatcccttg ccgcgcggct gcatagctat gatgtgctcg cgacttgtca gcacgacgcg
     7621 gccgatcgca accatgttca tcttttcgat gacgtcacgg atgacgacaa aagcgtcctg
     7681 ccccactttg ccatcgggcg cgatatagta cggacgaatg taatagaggt cgtcgatctc
     7741 ggacttgggc acaaattcat cgatctcgat tgttctggtg ctctcaagcg cgatcgtt
//
LOCUS       DATMZQ010000146         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_751051, whole genome shotgun sequence.
ACCESSION   DATMZQ010000146 DATMZQ010000000
VERSION     DATMZQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751051"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VN496_04840"
     CDS             complement(<1..262)
                     /locus_tag="VN496_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXF16305.1"
                     /translation="MAAGFITVSYIGAAILFILSLAGLSQQETARRGNIYGFTGMVIA
                     IVVTILALRSGNYAVLFPAMIVGALIGVFVASRVEMTQMPQLV"
     gene            complement(272..1825)
                     /locus_tag="VN496_04845"
     CDS             complement(272..1825)
                     /locus_tag="VN496_04845"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HXF16306.1"
                     /translation="MRIGIPKEIHAGEKRVAATPETVGQLVKLGYSVAIEAGAGQGAQ
                     FADDAYRAAGAEVVTDTRALWSQAQIVLKVRAPEQQPGLGVHEADLLREGAVLLSFIW
                     PAQNPELMERLKARRVTVIAMDAVPRISRAQKLDALSSMANIAGYRAVIEAAQHFGRF
                     FTGQITAAGKVPPAKVMVIGAGVAGLAAIGTARSLGAIVRAFDVRPEVKQQIESMGAE
                     FLELDFKEEGSGGGGYAKQMSPEFIKAEMDLFAAQARDVDIIITTALIPGKPAPKLIT
                     AEMVDSMRQGSVVVDLAAEQGGNCDYTRPGEVTVRNGVTIIGYTDLPSRLATQSSQLY
                     GTNLRHFLTDLTPQKDGNIVVNMEDDVIRGATVINQGTITWPPPPLKVPAIAAPPAAA
                     APKAAKAAEPSKGYGSLVAMALGALTLLLFGAYAPPAFMQHLTVFVLAVFIGFHVIWN
                     VAPALHTPLMSVTNAISGIIVIGALLQIGNPSTIVLVLAGISVLIATINIAGGFWVTQ
                     RMLKMFRRD"
     gene            complement(1860..>2165)
                     /locus_tag="VN496_04850"
     CDS             complement(1860..>2165)
                     /locus_tag="VN496_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF16307.1"
                     /translation="ESRIGIAQSRLLTLHAAHMMDTVGNKGAKAEIAMIKVAVPNMAC
                     KVVDWAIQAFGAAGLSNDHGIGVAYALARLLRIADGPDEVHRNQIGKLELAKYKQHR"
BASE COUNT          425 a          702 c          605 g          433 t
ORIGIN      
        1 ccaccagttg cggcatttgc gtcatttcta ctcgcgacgc gacgaacaca ccgatgagcg
       61 caccgacgat catcgccgga aacagcaccg catagttacc ggaccggagc gccaaaatcg
      121 tgactacgat cgcaatgacc atgccggtga agccgtagat attcccgcga cgcgcagttt
      181 cctgctggct caagccggcc aggctcagaa tgaacaagat cgccgcgccg atataggaaa
      241 ccgtgatgaa tcccgctgcc atgatgtcct cttagtctct tctgaacatc ttgagcatgc
      301 gctgggttac ccagaatccg cccgcaatgt tgatggtggc aatcaatacc gaaatccccg
      361 cgagcaccag gacgatcgtt gatggattac caatttgcag cagcgcgcca ataacgatga
      421 tgccgctgat tgcattagtc acgctcatga ggggcgtgtg caacgccggt gcgacgttcc
      481 agattacgtg gaagccgatg aagactgcga gcacgaacac agtgagatgc tgcatgaacg
      541 caggcggcgc ataggcgccg aacaacaaca gcgtcagcgc gccaagcgcc atcgcaacca
      601 gggaaccata gccttttgac ggctccgctg ccttggcggc tttcggtgcc gcagcagccg
      661 gcggcgccgc aattgccgga actttcaacg gaggcggcgg ccaggtgatc gtgccctgat
      721 tgatgactgt agcgccccgg ataacatcgt cctccatgtt gacgacaatg ttgccgtcct
      781 tctgcggcgt aagatcagtc aggaaatggc gcaggttggt gccgtaaagc tggctggatt
      841 gggtcgcgag ccggctcggc aaatcggtat agccgataat cgtgacgccg ttgcgcacgg
      901 tgacctcacc cggacgggtg tagtcgcaat taccaccctg ctccgcagcc aggtccacca
      961 ccacgctgcc ctgacgcatc gagtcgacca tctctgccgt aatcagcttc ggcgcaggct
     1021 tgccgggaat cagcgcggtg gtgatgatga tgtccacgtc cctggcctgc gcagcgaaca
     1081 gatccatttc cgccttgatg aactcgggac tcatctgctt ggcatagccc ccgccgccgg
     1141 aaccttcctc cttgaaatcc agctccagga attcggcgcc catgctttca atttgctgtt
     1201 tgacttccgg gcgcacatcg aacgcacgca caatcgcacc caggctgcgc gccgtcccga
     1261 tcgccgcgag ccccgccacc ccggcgccga tgaccatgac tttggcaggc ggcactttcc
     1321 ccgctgccgt gatttgaccc gtgaagaaac gtccgaagtg ctgcgccgct tcgatgacgg
     1381 cgcgatagcc ggcaatattg gccatggaac tcaacgcatc gagcttctgc gcgcgcgaaa
     1441 tgcgcggcac ggcatccatg gcgatcaccg tgacccggcg tgccttgagc cgctccatca
     1501 gttccgggtt ctgcgcaggc cagatgaaac tgagcagcac cgcgccttcg cgcaacaaat
     1561 ccgcttcgtg cacaccaagc cccggttgct gctccggtgc acggaccttg agcacgatct
     1621 gcgcttgcga ccacaacgcg cgcgtatcgg tcacgacttc cgcaccggcc gcccgataag
     1681 cgtcgtccgc aaattgtgca ccttgtcctg cgcctgcctc gatcgccacc gagtaaccca
     1741 gttttaccag ttggccgacc gtctccggcg tagccgcgac gcgcttctcg ccggcgtgga
     1801 tttccttggg aatgccgatt ctcatttcgc tgtgatctcc gcttttgatg tcacttgaat
     1861 caccggtgct gtttgtattt agccagttcc agtttgccga tctggttgcg atgcacttcg
     1921 tccggcccat cggcaatgcg cagcaaccgc gccagcgcat aagccacgcc gatgccatga
     1981 tcgttgctga gtccggcagc accgaacgcc tggattgccc aatcgacgac cttgcacgcc
     2041 atgttcggaa ccgccacttt gatcatcgcg atttcggcct tggcgccctt gttgccgacc
     2101 gtgtccatca tgtgggcggc atgcaatgtc agcaagcggc tctgggcgat gccgatgcgc
     2161 gactc
//
LOCUS       DATMZQ010000147         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_465440, whole genome shotgun sequence.
ACCESSION   DATMZQ010000147 DATMZQ010000000
VERSION     DATMZQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465440"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VN496_04855"
     CDS             <1..581
                     /locus_tag="VN496_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXF16308.1"
                     /translation="WPGVIVRELVLGMAIGLATNFVFEAAQMAGAILGVQMGYSLVNI
                     LDPQTQVDTTVVALFHQSVAFLLFLQLDVHHWILRGIAHSFEYLSPGAIHLNRGLTTE
                     LLHGVAGVLVTGLQIAAPVLAATLVADVILGFLGKASPQMPLMLLGPALKSMLGVSLL
                     ITVIHYWPALFERYFTESVEFSERLLHLAARS"
     gene            578..1648
                     /locus_tag="VN496_04860"
     CDS             578..1648
                     /locus_tag="VN496_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HXF16309.1"
                     /translation="MSQNKTEKPTPRRRQKAREQGQVARSRDLSGILALAGAFGVVAW
                     QGNSGIEVWRSLLRQAFQFSGADSLSPTAPVFVWTGWTLAKCVVPVMATAWTMSLLGG
                     LAQGGLVFAPEALIPKVERLSPGTKLKQIFSLTGLSGLLKSLLPFAAIAYVGFATIRD
                     HWIAIVIASNTSLWTFGRFLITLLFEVTWKSALVLGVWAFVDYFLTWRKSESDMRMSR
                     EEMRQETKDSDGNPQIKMRIRRLQRQMRKQLMLRETEKATVVVTNPTHFAVAILYSMD
                     MGAPVVIAKGRNRLAQKMKQIARWKDIPVLENPPLAQALYKTVDVGQSIPAKLYTAVA
                     EVLAFVYRAQAQVRRQGGVETR"
     gene            1648..>3072
                     /locus_tag="VN496_04865"
     CDS             1648..>3072
                     /locus_tag="VN496_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HXF16310.1"
                     /translation="MAAITKPKLAGGSKVDWIVPAAAVSMVFVMLVPMPGFLLDIMLA
                     FSMTASVLVLLTAVQILRPAQFSVFPSLLLLMTLFRLSLNLASSRRILLHGAEGASAA
                     GRVIEAFGQFVVGGNYVVGFVLFLALIAIQYIVVSHGAVRTAEVTARFTLDAMPGKQM
                     AIDADLNAGLIDEVQARARREQIAHEAEFYGAMDGAARFNQRDALATILITAINIIAG
                     FLIGVLQQGVPFMEALKTYTVLTVGDGLVTMIPSLLVSVAGGMVVTRASSDGTLSTDL
                     GKQLFGSSRPLWIAAAVMTGLALIPGLPKLSFILMAVIMAALARRATLEPEAEKPAAS
                     TSVGKSQGAAALALDPLDAALKLDDLMLEVGVGLVPLVDANKGGQLLTKVRALRKHLA
                     TQLGFLVPSIHITDNLTIKDLEYVVYLRGVEITRWEMRRDCLLAISSQPTPPALPGVD
                     TRDPAFGAPAKWITKDQQAQALAAG"
BASE COUNT          589 a          892 c          923 g          668 t
ORIGIN      
        1 agtggccggg agtgatcgtg cgagagttgg tgcttgggat ggccatcggt ctggccacga
       61 attttgtgtt tgaagcagcc cagatggctg gcgcgattct gggtgtgcaa atgggatatt
      121 cgctggtcaa cattctcgat ccgcaaaccc aggtggacac gacggtagtt gcgttgttcc
      181 atcagtcagt ggcattcctc ctgtttctgc aactggacgt acatcactgg atccttcgcg
      241 gcatcgcgca cagttttgaa tacttatcac ccggcgcgat ccatctaaat cgcggcctga
      301 ccaccgaatt gctgcacgga gtggcgggcg tgctcgtaac cggcctgcag atcgccgctc
      361 ccgtactggc cgcaaccctg gtggcagacg tgattctggg attcctgggg aaagcttcgc
      421 cgcaaatgcc tctgatgtta ttgggccccg cgctcaagag tatgttgggc gtgagcctgc
      481 tcatcacggt catccactat tggccggcac tgtttgagcg ttacttcacg gaatccgtgg
      541 aattttcgga gcggcttctg catctggcgg cacggtcatg agccagaaca agacagaaaa
      601 gccgacacca cggcgacggc aaaaggctcg cgagcaaggc caggtcgcac gttcgcgaga
      661 cttgtccggc attctggctt tggcgggcgc gttcggcgtc gtcgcctggc aaggcaattc
      721 gggaatcgaa gtctggcgaa gtctgttgcg gcaagcattt cagttttccg gcgcagattc
      781 gctttcccct accgctcccg tgttcgtgtg gaccggttgg acgctcgcca aatgcgtggt
      841 gccggtgatg gcaactgcgt ggactatgtc tttgcttgga ggcttggcac aaggcggtct
      901 ggtgtttgcg ccggaagcct tgattcccaa agttgaacgg ctcagtcctg ggacgaaatt
      961 gaaacagatc ttttccctca ccgggttgag cggacttcta aagtccttgc tgccgttcgc
     1021 agccatcgcc tacgtgggct ttgccacgat tcgcgaccac tggatcgcga tcgtgattgc
     1081 ctctaatacc agcctatgga ctttcggccg cttcttgatc acgctcctct tcgaggtgac
     1141 ctggaaatcc gcgctggtcc taggagtttg ggcgttcgtg gattacttcc tcacctggcg
     1201 aaagtcagaa agcgacatgc gcatgtcgcg ggaagagatg cggcaagaga ccaaagattc
     1261 agacggaaac ccgcagatca agatgcgcat ccggcgactg caacggcaga tgcgcaagca
     1321 actgatgttg cgtgaaacgg agaaagcgac cgtcgtagtt accaacccga cgcactttgc
     1381 ggtagcaatt ctttactcaa tggacatggg tgcgcccgtc gtaattgcca agggccgaaa
     1441 tcggctggcg caaaagatga agcagattgc gcgttggaaa gatattccgg tgctggagaa
     1501 tcctccgttg gcccaggcgc tgtacaagac ggtcgacgtt ggacaaagca ttccagccaa
     1561 gctctacacc gccgtggccg aagtcctggc attcgtgtac cgagcacagg cgcaggtcag
     1621 gcgacaggga ggggttgaga cacgctaatg gctgccatca ctaaaccgaa gctcgcagga
     1681 ggatccaagg ttgattggat cgtaccggcc gcggctgtca gcatggtctt cgtcatgctg
     1741 gtgccgatgc ccggctttct gctcgacatc atgctggcct ttagcatgac ggcatcggtg
     1801 ctggtacttc ttacggccgt ccagattctt cgtccagcgc aattttctgt cttccccagc
     1861 ctgctcctgt tgatgaccct gttccgcctg tcgctcaacc tggccagcag ccggcgaatc
     1921 ctgctgcacg gcgccgaagg cgcttcggca gcaggccgcg tgattgaggc cttcggccaa
     1981 ttcgtcgtgg gcggcaacta tgttgtcgga ttcgtcttgt tcttggctct gattgccatt
     2041 cagtacatcg tggtcagtca cggcgcggtg cgaaccgcgg aagttacggc gcgtttcact
     2101 ctcgatgcca tgcccggcaa gcagatggca atcgatgccg atctgaacgc cgggttgatt
     2161 gatgaagtgc aggcccgagc ccgccgcgag cagattgcgc acgaagcgga attctatggc
     2221 gcgatggatg gcgcagctcg cttcaatcag cgcgacgccc tcgccacgat tctgatcacc
     2281 gccatcaaca tcattgccgg cttcctcatt ggcgtcctcc agcagggggt gcccttcatg
     2341 gaagcgctga agacctacac ggtgctgacc gtcggcgacg gcctggtcac gatgattccc
     2401 tccctgctgg tgtcagtcgc gggcggcatg gtggtcacgc gagcgtcctc ggacggcacg
     2461 ttatcgacag acctaggcaa gcagctgttc ggcagcagcc gtccgttatg gatcgccgcc
     2521 gccgtgatga ccgggcttgc tctgattccc ggactgccga agctttcatt cattctgatg
     2581 gccgtcatca tggcggctct cgcgcgccgc gctacattag aacccgaggc tgagaagccg
     2641 gcagccagca cctccgtcgg gaagtcccag ggtgctgcgg cgctggcgct tgatccgctc
     2701 gacgcagcat tgaagttgga tgacctgatg ctcgaagtag gcgtcggcct cgtgccgctg
     2761 gtagacgcca acaagggcgg gcaattgctg accaaggtcc gagccctacg caaacacctg
     2821 gcaacacaac ttggcttcct ggttccctcg attcacatta ccgacaacct gaccatcaag
     2881 gatctggaat atgtcgtgta cctccgcggc gtagaaatta cgcgctggga gatgcggcgc
     2941 gattgcctgc tggctatcag ttcccagccg actccgcccg ctctgccggg tgtcgacacg
     3001 cgcgatcccg cattcggcgc acccgcaaag tggatcacaa aagatcagca ggcccaggca
     3061 ttggcggcgg gt
//
LOCUS       DATMZQ010000148         3477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_753032, whole genome shotgun sequence.
ACCESSION   DATMZQ010000148 DATMZQ010000000
VERSION     DATMZQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753032"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VN496_04870"
     CDS             <1..436
                     /locus_tag="VN496_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF16311.1"
                     /translation="VPIYPVKGYSVTVALTHPEKAPMMCLSDENAKIAISRLGSRLRA
                     AGTAELTGYDTSLSASRCNAILSRIEQLFPGAGDYAKASAWAGLRPATPSNVPLIGRT
                     GYRNLYLNTGHGTLGWTLACGSGQAAADLIAGRQPQVNFPFS"
     gene            577..1863
                     /gene="hisD"
                     /locus_tag="VN496_04875"
     CDS             577..1863
                     /gene="hisD"
                     /locus_tag="VN496_04875"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXF16312.1"
                     /translation="MAIFLKTGISAEQDAADIARVRKTVEDILADIEKRGDVAVRELS
                     KKFDNWAPDSFRMSKGEIEDCVRQLPRQVVDDIKFAQEQIRNFAQIQRDALRDVEVET
                     LPGVVLGHKNIPVNSVGCYVPGGKYPLVASAHMSIVTARVAGVKRIISCAPPYQGKPH
                     PAIVGAMHLGGADEIYTLGGVQAIGAMALGTATLKPTDMIAGPGNAFVAEAKRQLSGR
                     VGIDLFAGPTETLIIADDSCDAEMCATDLLGQAEHGPNSPAILLTNSRKIAEDTIKEV
                     GRQLQTLPTAEIAGQAWKDYGQVILCKDYAEMAREADRIASEHVQVMTKDPDYFLRNM
                     TNYGALFLGPETNVSYGDKVIGTNHTLPTRKAARYTGGLWVGKFIKTCTYQRVKPEAS
                     AMIGEYCSRLCAVEGFAGHKAQADLRVKRYGRLRAA"
     gene            2193..2696
                     /locus_tag="VN496_04880"
     CDS             2193..2696
                     /locus_tag="VN496_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16313.1"
                     /translation="MKAILVGGMAAVAMIAADAAIAAKVNIPKEGSYAFDFCPIGRGK
                     TFSSGDKFFVMNYDLDAIVRSTPSGGAFDRMGARCYGIYANINDRQREAGICEITDLD
                     GDKWFMDYHGNADGKGGTYTAAYGTGKYDGVIIQGEYRLDNNWGSPSKEVSFLGCNPN
                     KGTYKLK"
     gene            2816..>3477
                     /locus_tag="VN496_04885"
     CDS             2816..>3477
                     /locus_tag="VN496_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF16314.1"
                     /translation="MSKRASFAKISRPRLFGVVARERLFSVLDENRGRPLIWISGPPG
                     AGKTALVASYLEERAYPTVWYQIDAGDADPASLFHYLALAAEPITKAQSAPLPRFVPE
                     HLSDLPSFARLFFRAFFAQLPEGGVLVLDNYQDTPLDASLHEIVRQAIAEVPPGHSVI
                     GISRVEAPPSFVQFAATGFMIDVRWDKLQLTFEEVRAIAAKRSVTDDWLLKALHQQSQ
                     GWA"
BASE COUNT          757 a          968 c         1050 g          702 t
ORIGIN      
        1 cgtgccgatc tatccggtca aagggtattc ggtgaccgta gcgctgacgc atccggagaa
       61 ggctccaatg atgtgcctga gcgacgagaa cgccaaaatc gccatttcaa gattggggtc
      121 acgcctgcgg gccgccggca cggctgaact gaccgggtac gacacctcgc tgagcgcctc
      181 gcgctgcaac gcgatcctgt cccggatcga gcagcttttc cctggcgccg gagactatgc
      241 caaggcgtcg gcatgggctg ggctgcgtcc ggcaacgccg agcaacgtgc cgctgatcgg
      301 gcgtaccggg tatcggaatc tgtatctcaa taccggacat ggcacgctgg gctggacttt
      361 ggcctgtggc tccgggcagg ccgccgctga cctcattgcc ggacgccaac ctcaggtgaa
      421 tttccctttt agctaacggg tctaacagac tcgaattgtt tgacgctaac ggcgcacagc
      481 tagcaaaata gcaggtcttg cctgcttgcc aagcggcccg gcaggcaggg cgcccactat
      541 ttgataccca tcgtttcagc ccgcggaaaa aataccatgg ccattttctt gaagaccggc
      601 atttcggccg agcaggacgc tgccgatatt gcccgggtac gtaaaactgt cgaggacatc
      661 cttgccgaca tcgaaaagcg cggcgatgtt gccgtgcgcg aactctcgaa gaaattcgat
      721 aactgggcac ccgacagctt ccgcatgagc aaaggcgaaa tcgaggactg tgtgcgccag
      781 ttgccgaggc aagtcgtcga cgatatcaaa tttgcccagg agcagattcg caattttgcc
      841 cagatccagc gtgacgcctt acgcgatgtg gaggttgaga ccctgcctgg cgtggtactg
      901 gggcacaaga acattccggt caacagcgtc ggctgctacg tgccgggcgg gaaatatccg
      961 ttggttgcgt ccgcgcatat gagcattgtg accgcgcgcg tggctggcgt gaagcgcatt
     1021 atctcgtgcg cgccgcctta ccagggcaag ccccatccgg ctatcgtggg agccatgcat
     1081 ctgggcggcg cggatgagat ttacacgctt ggcggtgtgc aggcgattgg tgcgatggcg
     1141 ctgggcaccg cgacgctgaa gccgaccgat atgatcgccg gcccgggtaa cgcatttgtc
     1201 gcagaagcta aacgtcagct gtccggtcgt gtcggcatcg atctgttcgc tggtccgacc
     1261 gaaacgctga tcatcgccga tgacagttgt gatgcggaga tgtgcgcgac cgatctcttg
     1321 ggccaggcgg agcatggacc caactcgccc gccattctat tgaccaattc gcgcaagatt
     1381 gcagaagaca ctatcaagga agtcgggcgc cagttgcaga ccttgccgac cgccgagatt
     1441 gccggccagg cctggaagga ttatgggcaa gtgatcctgt gcaaggacta cgcggaaatg
     1501 gcccgggaag ccgatcgcat cgcttccgag catgtgcagg tcatgaccaa ggatccggat
     1561 tactttctgc gcaacatgac caactacggc gcgctgtttc tgggtccgga aaccaatgtc
     1621 tcctacggag ataaagtgat cggcaccaat cacacgttgc caacgcgcaa agccgcgcgc
     1681 tacaccggtg ggctgtgggt cggcaaattc atcaagacct gcacctatca acgggtgaag
     1741 ccggaagcct ccgcaatgat tggcgaatat tgctcgcggt tgtgcgccgt ggaaggcttc
     1801 gcgggacaca aggcgcaggc cgatctgcgg gtaaaacgtt atgggcgcct tcgcgctgcc
     1861 tgaagaaccg taaagcagcg gtacctcttc ggactcctga aaacagccgc tgcgccggct
     1921 gtttttgttt ttcctttatc tgtagccaat ccgtagccat cctgacgcta gcctgattgc
     1981 acgccagcgg ggaggtgcat ggacgaattg ggcgtatacg tagttcgggt ctaccgaaag
     2041 gatgacgaag gcatgactgg gctcgttgag tcggtggcga gcggcgaaca attgtcgttt
     2101 cataccacgc aggaactctg gagcgcactc agaaatctgc ctaccccccg gcgacatact
     2161 gcagatgaac ctaacgataa ggagggtcac acatgaaagc aattttggtg ggagggatgg
     2221 cggccgtcgc gatgatcgca gcagacgccg cgatagcggc caaggtcaac ataccgaagg
     2281 agggcagcta cgccttcgac ttctgtccca taggtcgtgg caaaactttt tcatcgggag
     2341 acaagttttt tgtgatgaac tatgacctcg acgcgatcgt aaggagtact ccctccggag
     2401 gagcgttcga tcgcatggga gcccggtgct acggcattta cgccaatata aacgatcgcc
     2461 agcgtgaagc gggtatatgc gagattacgg atctcgatgg cgacaaatgg tttatggact
     2521 accacggcaa cgcggatggc aagggcggta cctataccgc tgcatacggc actggcaagt
     2581 atgacggcgt aatcatacaa ggcgagtatc gactggataa caattggggc agcccgtcca
     2641 aagaagtcag tttcctggga tgcaacccta acaagggcac ttacaagctg aagtaaggac
     2701 cgtgtgacct tgcggttgct tgagtgccga gcggaggatg cgcatactgg cgcgcagtag
     2761 tgcgagtaca ggcacgatcg actcttgcgc cagagagccg agaaaggact ccgggatgag
     2821 caagagagcg tccttcgcga aaatcagccg tccacgcctg ttcggagtgg tggcgcgcga
     2881 gcggctgttc tcggtgctgg atgaaaaccg cggtcggccg ctgatctgga tttccggtcc
     2941 gcccggtgcc ggaaaaaccg cgctggtcgc gagctacctc gaagaacggg cctatcccac
     3001 cgtttggtat caaattgatg ccggagacgc tgacccggct tctctgtttc actaccttgc
     3061 attggcggcc gagcctataa ctaaagccca atcggctccg cttccacgat ttgtccccga
     3121 gcacttatcc gatttaccga gcttcgctcg tttatttttc cgcgcatttt tcgcacagtt
     3181 gcccgaaggt ggagtactgg tgctggacaa ttatcaggac acaccgctgg atgcgtccct
     3241 acacgagatc gtgcgccagg ccattgcaga agtgccgccc ggtcactcag tcatcggaat
     3301 cagccgggtc gaagcgcctc ctagctttgt tcaattcgcg gcaacgggat tcatgatcga
     3361 tgtgcgctgg gacaagctgc aactcacctt tgaagaagtg cgcgccattg cggcaaaacg
     3421 aagcgtgacg gatgactggt tgcttaaggc actgcatcag caatcccagg gatgggc
//
LOCUS       DATMZQ010000149         3170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1157119, whole genome shotgun sequence.
ACCESSION   DATMZQ010000149 DATMZQ010000000
VERSION     DATMZQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157119"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN496_04890"
     CDS             complement(<1..381)
                     /locus_tag="VN496_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXF16315.1"
                     /translation="MSQPQLPSRGWLKPWMVAAALVVVASIGVNLVATTLGYSPPWLL
                     GANLVVALFMLGASILAVAGLASRTEEEASRMQVALDASQARLAAIVDSAMDAIVTVD
                     ERQRVVLFNRAAEQVFRCRREDALG"
     gene            503..1897
                     /gene="hemN"
                     /locus_tag="VN496_04895"
     CDS             503..1897
                     /gene="hemN"
                     /locus_tag="VN496_04895"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HXF16316.1"
                     /translation="MIYPPSELVIDPVLIRKYDISGPRYTSYPTADRFVEAFGEATYR
                     YWLSSRNIGGIINPLSLYLHVPFCSNVCFYCACNKQVTRDRSKAVQYVKYLQREVGLT
                     ADLLNDSREVSQVHWGGGTPTYLSRDDTRSLCKMLAAQFDIAPDCEYSIEVDPREIQP
                     DSMAFLAELGFNRVSLGVQDFDPAVQKAIHRVQPVEMTGRVTEAARTAGFRSINFDLI
                     YGLPKQSLNSFNATLDSVLALDPDRIALYSYAHLPAKFPPQRRIVESELPSAETKLQI
                     LTLAIGRLTGAGYVYIGMDHFSKPADELAVAQRQGRLHRNFQGYSTRPDSDMLAFGTS
                     AISRVGPSYSQNVREIKDYDAALDADRLPVRAGLVLSKDDLVRRAVIQALLCQFRLSV
                     ESIETAYLVNFASYFAGELEDLKQFEEEGLVEIQPDWIVVTPRGRLLVRIIGMIFDRY
                     LREGSRWAGYSKVL"
     gene            1908..2600
                     /locus_tag="VN496_04900"
     CDS             1908..2600
                     /locus_tag="VN496_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXF16317.1"
                     /translation="MPEATLFAVFIAGLLGGGHCAAMCGGIVSALSVQRGARFSFHLA
                     YNAGRVGSYAVAGAIAGAIGGMVLLNEALSIRIAMYVVANGLIVLMGLYLAGGPALIT
                     RFEALGRMPWRYISRLTRGLLPVDTWPRALLAGSIWGWLPCGLTYSVLAIALVSGSGG
                     RGAALMAAFGLGTLPNLLAAGMLITRARPWLQQPMVRRISGCVLLGFGVAGMAQATGL
                     GEQIRRGVLCLF"
     gene            2816..>3170
                     /locus_tag="VN496_04905"
     CDS             2816..>3170
                     /locus_tag="VN496_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF16318.1"
                     /translation="MATTYTPEFEKSTYGKVAWHLIPFLFLCYIVAFLDRVNVGFAKL
                     QMAPDLKFSDTVYGFGAGIFFIGYFIFEVPSNVLLERLGARVWIARIMITWGLISSAF
                     VFTPHIHWGVIATMFN"
BASE COUNT          612 a          924 c          936 g          698 t
ORIGIN      
        1 gcccagggca tcttcgcgac ggcaacgaaa cacctgttcc gccgcgcgat tgaacagtac
       61 gacacgctgt cgctcgtcta ccgtaacgat cgcatccatc gcggaatcaa cgatcgcggc
      121 cagacgggcc tgggaggcat ccagggcaac ctgcatgcgc gaggcttctt cttccgtacg
      181 cgaagcgagc ccggccactg cgaggatgct ggcgccgagc atgaaaagcg ccacgacgag
      241 attcgcgccc aataaccagg gcggactgta tcccagggtg gtcgcgacca gattgactcc
      301 gatgctggcg acaaccacga gcgcggcggc gaccatccac ggtttcaacc agccacgcga
      361 gggcagctgt ggctggctca tgcagcgaaa gcccgtgtgc tggccgaaca accaaagggc
      421 aatgcgttga tgcaggtcaa tgtctttacc actttaccgg gcatagtctc atgttcatga
      481 agcagccgct ccaatccagc agatgattta ccccccgtca gaactcgtca ttgatccagt
      541 ccttatccgg aaatatgaca tcagcggccc gcgatatact tcttatccta ccgcggatcg
      601 tttcgtcgag gctttcggcg aagcgaccta ccgatattgg ctttccagcc gcaatatcgg
      661 cggcatcatc aacccgcttt ccctctattt gcacgtgccc ttctgcagta acgtgtgctt
      721 ctactgcgcg tgcaacaagc aggtgacacg cgaccgcagc aaggccgtcc agtatgttaa
      781 gtatttgcag cgggaagtcg gccttacggc cgatcttctc aatgatagcc gggaagtctc
      841 ccaggttcac tggggcggcg ggacgccgac ttacctcagc cgggacgaca cgcgctcgct
      901 gtgcaaaatg ctggccgctc aattcgatat cgcaccggac tgcgaatatt ccattgaagt
      961 cgatccgcgc gaaattcagc ctgactccat ggcgtttctt gcggaactcg gttttaaccg
     1021 cgtgtccctc ggcgttcagg atttcgatcc ggcagtgcag aaagcgatcc atcgcgtcca
     1081 gccagtggaa atgacgggcc gggttaccga ggcggcgcgc acagcaggct ttcgctccat
     1141 caactttgac ctgatctacg ggctgccaaa gcaatcgctc aacagtttca acgcaacgct
     1201 cgatagcgtg ctggctctcg atcccgaccg tattgcgctc tacagctacg cgcatttacc
     1261 ggccaagttt ccgccgcagc ggcgcatcgt tgaatctgaa ttgccttcgg ccgaaaccaa
     1321 actgcagatt ctgacccttg cgatcggccg cctgaccggg gcgggatacg tgtatatcgg
     1381 catggatcac ttttcgaaac cggcagacga acttgcggtt gcgcaacgcc agggacgctt
     1441 gcatcgtaat tttcagggct attcgacgcg gccggattcc gacatgctgg cgtttggcac
     1501 ttcggcgata agccgagtgg gaccgagcta cagccagaat gtccgggaaa taaaagacta
     1561 cgatgccgca ctcgatgcag accgtcttcc ggtacgggcc gggttggtgt taagcaagga
     1621 tgacctggtg cgccgtgccg tgatacaggc gctgctgtgc cagtttcgcc tcagtgtcga
     1681 atcgatcgag actgcttacc tggtaaattt cgccagctac tttgcagggg aactcgagga
     1741 cctgaagcag ttcgaggagg aggggctggt ggaaattcaa ccggactgga tcgtggttac
     1801 gcccagggga agactgctcg tgcgcatcat cggcatgata ttcgatcgct acttgcgcga
     1861 aggcagccgc tgggctggtt attccaaagt gttgtagttg gcgagccatg ccagaagcaa
     1921 cgctgtttgc agtatttatt gcgggcctgc ttggcggcgg gcattgcgcc gcgatgtgtg
     1981 gcggcatagt gagcgcgctg tccgtgcaac gcggcgctcg gttttccttc cacctggcat
     2041 acaacgcggg gcgtgtcggc agctatgccg tagccggcgc gatcgcgggc gcgattggcg
     2101 gcatggtatt gctgaatgaa gcgctttcaa tacggatagc gatgtatgtc gttgccaacg
     2161 ggctgattgt gctgatgggc ctctatctcg cgggtggccc ggcgctgatc acccgtttcg
     2221 aagcgctcgg gcgcatgcca tggcgctaca tcagccgtct gacccgtggc ctgctcccgg
     2281 tcgatacctg gccgcgtgct ttgctggccg gatcgatctg gggctggttg ccctgcgggt
     2341 tgacgtacag cgtacttgcg atcgctcttg tcagcggtag cggtggtcgc ggtgccgcgc
     2401 ttatggcggc gttcgggttg ggcacgctgc cgaacctgct cgcggccggc atgctgatca
     2461 cacgtgcgcg gccatggctt cagcaaccga tggtgcgccg catttccggt tgcgtgctgc
     2521 tcggctttgg cgtcgcaggc atggcacagg ccaccggctt gggcgagcag atccgtcgcg
     2581 gggtcctttg cctgttctga tcccggctgc agcgcaagaa tttgcttcgt cgcgtacttc
     2641 aatatcgcgc tagggtctgg ccaatagccc cgtaagccgt gtttttgttt acactgctcg
     2701 caagccgggt gggctgggca ccgcaagcaa ccagcaaggg ctctccgacc aggccgacaa
     2761 aaaaaacaat gcaccttaaa agcgagtgaa agaacaacac ggaggagtag ataaattggc
     2821 taccacttat accccggaat tcgaaaaatc gacctatggc aaggtcgcct ggcatctgat
     2881 cccttttctg ttcctgtgct acatcgtcgc tttcctggat cgggtcaatg tcggtttcgc
     2941 caagctgcag atggcgcccg atctgaaatt cagcgatacc gtttacggct ttggcgccgg
     3001 tattttcttt atcggctact tcatcttcga agtgcccagc aacgtcttgc tcgagcgcct
     3061 gggcgcacgc gtctggatcg cgcgcatcat gattacctgg ggcctgatct cgtcggcttt
     3121 cgtgttcacc ccgcatattc actggggagt cattgcgacg atgtttaatt
//
LOCUS       DATMZQ010000150         8280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_121931, whole genome shotgun sequence.
ACCESSION   DATMZQ010000150 DATMZQ010000000
VERSION     DATMZQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121931"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VN496_04910"
     CDS             <1..394
                     /locus_tag="VN496_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXF16319.1"
                     /translation="IADAQGVGAALALGAAAAQIGTAYLLCPEATISAIHRAALKSEA
                     ARHTALTNIFTGRPARGIVNFAMKELGPINRDAPAFPLATSSMAPLRAKAEKEGSGDF
                     TPLWSGQNATGCKEVPAAQLTRELAGGL"
     gene            complement(435..1910)
                     /gene="acpA"
                     /locus_tag="VN496_04915"
     CDS             complement(435..1910)
                     /gene="acpA"
                     /locus_tag="VN496_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HXF16320.1"
                     /translation="MTSARRLVQGLAVLLLLSGFGSRSGDADAGSIARIKHIVVIYAE
                     NRSFDHLYGLFPEANGIGNALDRYVAQVDHDGTAFKVLPPVWADEHNLKSRQIDPRYP
                     ATLPNRPFRIDQPPVNSPLDIRTRDLVHRFYQHKEQINGGRNDHFVSMSNAGALVMGY
                     YDGSRLPMWSIAREFTLADNFFMGAFGGSFLNHFWLVCACTPEYRDAPDDVRAVEDRN
                     GNLVRQNDSPASAMDGPVLLKDGAVTPAGYAVNTLQPPYQPSAIAPALQGDPALADPD
                     NVRRLPPQTLTTIGDTLSAKHVSWAWYAGSWNRALADRSIIYNENNVNFQPHHQVFNY
                     FARFAPGTAERERHLRDGEAFLADIEAGRLPRVAFYKPTAKVNAHPGYTDVLSGDMHI
                     AEVVERIRKNPQLWKDVAIIVTYDENGGFWDHVAPPAGDRWGPGSRVPTIIVYQFSRK
                     GFVDHTLYDTTSILKFITLRFGLQPLPGVRANAGDLTNAFE"
     gene            complement(1941..2423)
                     /locus_tag="VN496_04920"
     CDS             complement(1941..2423)
                     /locus_tag="VN496_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16321.1"
                     /translation="MSFRSWRIHLPFPLPVKQLASLLWRPACIACFLVVHSVSAQPAA
                     DHPILGTWTFSVAGGACAESYDFRVNGTLVTSSGKARSERVFEISASPDASGFYTLVD
                     KIVSENGKPDCFGQPTVAGVSALVYIRFSTSQDRFVACETESPDNCIGPLHRIRRNRI
                     "
     gene            2552..3334
                     /locus_tag="VN496_04925"
     CDS             2552..3334
                     /locus_tag="VN496_04925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16322.1"
                     /translation="MEIKLLSHELALVTGAASGIGRGIAAALAREGARVALCDIDVPA
                     GEDAAAALRREGCDAVFMAADLAALDGVERLLKDDLARFGTPSILVHAASPRRMEADN
                     ILSVDTETWDRMLAVNVRSGFLLAREIARSMMNKSIRGRMLFTTSLHAESPRNLPHYS
                     AAKAAQTMVVKELAKALGPHGIRVNAIAPGAVPGGGFDASGAGIERLIRCTSLRRLGT
                     PEDIAGMAVALLVDRFSAYVTGTTVVVDGGMALHNWLEPAEL"
     gene            3382..3870
                     /locus_tag="VN496_04930"
     CDS             3382..3870
                     /locus_tag="VN496_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXF16323.1"
                     /translation="MSKANSAGILLYRLRSAAEVFLVHPGGPFWARKDAGAWSIPKGE
                     YEQGADALEAAKREFNEETGCAITGNFIMLVPLKQRSGKTVHAWAVEGDLDATSITSN
                     LFSMEWPPRSGKQQQFPEIDQGAWFTLPAAKEKILADQRGFLDELERLLTGRNPVREQ
                     AP"
     gene            complement(3973..5133)
                     /locus_tag="VN496_04935"
     CDS             complement(3973..5133)
                     /locus_tag="VN496_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF16324.1"
                     /translation="MTDLAPLPQKVLPPGIRSRFVNDVNGLRMHILEAGYEQQNRPLL
                     LLLHGFPELAYSWRKVMVPLAAAGYHVVAPDQRGYGRTSGWDGNYDGDYTSFRMLNLV
                     RDALSLVSALGYRHVQGLFGHDSGANVSAWAALIRPDVFRCVTLMSAPFGGPPALPFD
                     TDRNGPTSPPATSIHDDLAALKRPRKHYQWYYSTRDANDDMWKCPQGVHAFLRAYYHH
                     KSADWKQNKPFPLAAWRASELEKMPTYYIMDLHENMAQTVAHEMPIPEAIAANQWLPD
                     QELAVYAEEYRRNGFQGGLQWYRCNTSQKLQADMQIFSGRTIDVPSCFIAGKSDWGIY
                     QRPGADMIKMQKDVLTNMLGGCHLVDGAGHWVQQEQPQQVSDLLLRFLRKQT"
     gene            5256..6437
                     /locus_tag="VN496_04940"
     CDS             5256..6437
                     /locus_tag="VN496_04940"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXF16325.1"
                     /translation="MSAHGFTLFDTAIGRCGIAWSDQGIVGVQLPETRERETLGRLLK
                     HYPDATQGTPPPAIQHALDNIISLLAGESADLSEAALDMRHVPPFHRRVYDVARAIAP
                     GETISYGEIARQIGAGGSARAVGQALGRNPFAIVVPCHRVLAAGGKMGGFSANGGIGT
                     KLRMLSIESAQVNGHDTMFEGDGTFGFDPVVAVAHLRESSAFMAGLIDAVGPLRMRLK
                     RTPSIFVALAEAIVYQQLTGKAAATIYARVCALFPRAHEGPTPEKIWRVSPEKLRAAG
                     LSNAKLLALKDLARKSLDGDIPTLSQVHGMEDEAIIERLVKVRGIGRWTVEMLLISRL
                     GRSDILPVDDYGVRKGYALAFRKRNLPAPKALAKLGVQWKPYRSAASWYFWRALELPK
                     K"
     gene            6581..7426
                     /locus_tag="VN496_04945"
     CDS             6581..7426
                     /locus_tag="VN496_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXF16326.1"
                     /translation="MLIRTELKKGPVSIIDYRCTAGPTEKPFTEVHQSCSVSYVRQGS
                     FGYRTRGKVFELVTGSVLVGKAGEEFTCTHDYHGCGDECLAFHLDPEAADAIGWRTGN
                     WQSTGLPPLPELMVIGELAQTAADGTSNVGIDEIGWLFPLRLAQVLSGRLQDPQKAGS
                     RDRRRAVEAALWLETHAHEEIDLEGAARQADMSSFHFLRVFAKVIGVTPHQYLVRSRL
                     RHASRLLAADERPITDIAFDVGFADLSNFVRTFHRAAGVSPRAFRKAAKGERKIFQDR
                     LALLS"
     gene            7457..7819
                     /locus_tag="VN496_04950"
     CDS             7457..7819
                     /locus_tag="VN496_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF16327.1"
                     /translation="MYDHIGLKVKDLEAAVRFYAAALAPLGHVVGPSDKSYAGIGPKN
                     APGLWLHAASGSTTSGTHLAFRASDRDAVDRFHAAGLKAGGRDNGTPGLRIDYSPDYY
                     AAFLFDPDGNNVEAVFIK"
     gene            7890..>8280
                     /locus_tag="VN496_04955"
     CDS             7890..>8280
                     /locus_tag="VN496_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXF16328.1"
                     /translation="MTTIQEGGCACGDVRYRTVGDPVLATVCHCKYCQKRTGSAFSEP
                     VMFRIEQVEFSGTARTIYEHQSDESGRWLRMEFCPRCGTTVSWTAQRRPGMRGISGGT
                     FDNPDWVKGRKHIWTRSVQQWMLIPEDA"
BASE COUNT         1713 a         2377 c         2509 g         1681 t
ORIGIN      
        1 cattgccgat gcacaaggcg ttggcgcggc cctggcgctt ggtgccgctg ctgcgcaaat
       61 tggaacggca tatctgcttt gtcccgaagc caccatcagt gcaatccatc gcgccgcgct
      121 gaaaagcgaa gcggcccgcc acacggcgct gacgaacatc tttaccggcc ggccggcgcg
      181 cggcatcgtg aactttgcaa tgaaggagct cggaccgatc aaccgcgatg cgccggcatt
      241 tccgctggcg acttcatcca tggcgccatt gcgtgctaaa gccgagaaag aaggttcagg
      301 cgatttcacg ccgctgtggt cagggcagaa tgcaaccggc tgcaaggaag tgccggccgc
      361 gcagctcaca cgggaactcg cgggaggact gtagcctgac gcaatcgcgt caggctagca
      421 attgctggtg gcggtcattc aaacgcattg gtcaggtcac ccgcattggc gcgcacgccc
      481 ggcaagggct gcagtccgaa gcgcagggtg ataaatttca ggatcgacgt cgtgtcgtac
      541 agggtgtggt ccacgaatcc cttgcgcgaa aactggtaaa cgatgattgt cggcacgcgg
      601 cttcccggtc cccatcgatc gcccgcgggt ggcgccacgt gatcccagaa tccgccattt
      661 tcgtcatagg tgacaatgat ggccacgtcc ttccagagct gggggttttt gcggatgcgc
      721 tcgacgacct ctgcgatatg catgtcgcca gagaggacgt ccgtatagcc cggatgcgcg
      781 ttgactttcg cggtcggttt gtaaaacgca acccgcggca gacgcccggc ttcgatgtcc
      841 gccaggaatg cttcgccgtc gcgcaaatgg cgttcacgct cagcggtacc tggagcaaac
      901 cttgcgaaat agttgaacac ctggtggtga ggctggaagt tgacattatt ctcgttgtaa
      961 atgatggaac ggtctgccag cgcgcgattc cagctgccgg cgtaccaggc ccacgaaaca
     1021 tgcttggcag aaagcgtgtc accgatggtt gtcagggtct ggggaggaag acgacgcacg
     1081 ttgtccgggt cggcgagtgc ggggtctcct tgcagcgcag gcgcaatggc gctgggctga
     1141 tacggcggct gcaacgtgtt gaccgcatac ccggccggag tcactgcgcc atccttgagc
     1201 agcacagggc catccattgc ggaagctggc gaatcgttct gtcgcaccag attgccgttt
     1261 ctgtcttcga ccgcgcgaac atcgtccggc gcgtcgcggt attccggtgt gcacgcacag
     1321 acgagccaaa agtgattcag aaacgaaccg ccgaacgccc ccatgaaaaa attgtccgcc
     1381 agcgtaaatt cacgtgcaat ggaccacatg ggcaggcgag agccgtcgta gtagcccatg
     1441 accagcgcac ccgcattcga catagagaca aaatggtcgt tgcggccgcc gttgatctgt
     1501 tccttgtgct ggtaaaaacg gtggaccagg tcgcgggttc tgatatccag cgggctgttc
     1561 acgggcggtt ggtcgatcct gaacggccga tttggcaagg tcgccggata gcgcggatcg
     1621 atttggcggc ttttcagatt gtgttcgtcg gcccataccg gcgggagtac cttgaaggcc
     1681 gtcccgtcgt gatcgacctg tgcaacataa cgatccaatg cattgccgat gccattggct
     1741 tctggaaaca agccgtacag gtgatcgaag ctgcggtttt cggcgtagat caccacgata
     1801 tgtttgatcc tggcgatcga cccagcatcc gcatcacccg aacgcgagcc aaatcccgac
     1861 agcaacaaca ataccgcgag accctgcaca agccgacgag ccgaagtcat tgtgccgcga
     1921 tgtcctcagt ttgatgttgg ctaaatccgg ttcctgcgaa tgcgatgcag cgggccaatg
     1981 caattgtccg gtgactcggt ttcgcaggcc acgaatcggt cttgggacgt actgaagcgg
     2041 atgtagacaa gagccgatac acccgccaca gtcggttggc caaaacagtc cggtttgccg
     2101 ttctcgctga caatcttgtc caccagggtg taaaagccgc tcgcatccgg tgacgcagag
     2161 atttcaaata cgcgctcgga tctggccttt ccactgctgg tgaccagtgt tccgttcacg
     2221 cggaaatcgt aactctcagc gcaagctcca cccgctacgc tgaaagtcca tgtacccagg
     2281 atcggatggt ctgcggccgg ttgcgccgat acagaatgaa caacgagaaa gcaggcgata
     2341 cacgccggcc tccacagcaa acttgccagt tgcttcaccg gcagcggaaa tggcaaatga
     2401 attcgccaag accggaagct catgattcca tcgtccattc atgcatcacg atcatattgt
     2461 tcgggatgat caatcaattc gagtcaatta tgcaatcatc caggcacggt aattttggcc
     2521 agccattgtg gccggcgtaa aggagagcac catggagatt aagttgcttt cgcacgagtt
     2581 ggcactggtc acgggtgcag catccggcat tggccgtgga atcgctgcag cgctggcgag
     2641 ggaaggcgcg cgcgtcgccc tttgcgacat cgatgtgcca gccggcgagg acgccgcggc
     2701 agcactgcgc agggaaggct gcgatgccgt tttcatggcg gccgaccttg cggcgttgga
     2761 tggcgttgaa aggctgctga aagacgatct cgcccggttc ggcacgcctt caatcctggt
     2821 ccatgctgcg agtccgcgcc gcatggaagc cgataacatt ttgagcgtcg atactgaaac
     2881 ctgggacagg atgctggcag ttaatgtgcg ctccggtttc ctgcttgcgc gtgaaatagc
     2941 gcgcagcatg atgaacaaga gcatccgggg ccgtatgcta tttaccactt cattgcacgc
     3001 tgagagcccg cgcaatttgc cgcactatag tgcggcgaag gccgcacaga ccatggtcgt
     3061 gaaagaattg gccaaggctt tgggccccca cggcattcgc gtcaacgcga tcgcgcccgg
     3121 tgcggtaccg ggcggcggct ttgatgcaag cggcgcgggt attgaacgct tgattcgctg
     3181 caccagcttg cgccggctcg ggacgcccga agacattgct ggcatggcgg tggcgcttct
     3241 ggtggaccgg ttttctgcat acgtgaccgg cacgacggtg gtggtcgatg gtgggatggc
     3301 actgcacaac tggctggagc cggctgaact ataaaagctc cggtaaaggg aaccggcgtt
     3361 tactccaggg gaacaaaatc gatgtcgaaa gcaaatagcg ccggcattct gctttatcgg
     3421 ttgcgctcgg cagcggaagt ttttctcgtg catcccggtg ggccgttttg ggccaggaaa
     3481 gacgcgggcg cctggtcaat tccgaaggga gaatacgagc aaggcgccga tgcactggaa
     3541 gccgccaagc gcgaattcaa cgaggaaacc ggctgcgcca tcacaggcaa cttcatcatg
     3601 ctggtacccc tgaagcagcg cagtggaaaa acggttcatg cctgggcggt cgagggagat
     3661 ctcgacgcga cctccatcac cagcaatcta ttctcaatgg agtggccgcc gcgttcgggt
     3721 aagcagcaac aatttcccga aatcgaccag ggcgcctggt tcacattgcc cgcggcgaaa
     3781 gagaaaattc tggcagatca acgcggattt ctggacgaac tggagcggct cttgaccgga
     3841 agaaatccgg tgcgcgagca agcaccgtaa cctgcaaacc ggggacgatg gcatcgatcc
     3901 cgtcaaaaag ccttgcgccg gcccgatatc actctgctcg acccagtcac aaatacgtga
     3961 gcggctgtcg cgtcaggttt gcttgcgcaa aaatctcaat agcaagtcac tgacttgctg
     4021 tggctgttcc tgttgtaccc agtggcccgc tccatcaacc aggtgacagc cgccgagcat
     4081 gttggtcagc acatccttct gcatcttgat catgtcggca cccggccgct ggtaaatacc
     4141 ccagtcgctt tttcccgcga tgaagcagga cggaacgtcg atcgtgcggc ccgagaagat
     4201 ctgcatgtcg gcctggagtt tctgactggt attgcagcga taccattgca ggccgccctg
     4261 aaagccattg cggcggtact cttccgcgta cacggccaac tcttgatcgg gtagccactg
     4321 gttcgcggcg atcgcttccg gaatgggcat ttcatgcgcg actgtctgcg ccatattttc
     4381 gtgcaaatcc atgatgtaat aggtcggcat tttctccagt tcgcttgcgc gccaggcggc
     4441 aagcggaaaa ggcttattct gtttccagtc cgcgctcttg tggtggtagt aagcccgaag
     4501 gaaagcgtgc acgccttgtg gacatttcca catgtcgtcg ttggcgtcac gcgtcgagta
     4561 gtaccactgg taatgcttgc gcggacgctt gagcgctgcc aggtcgtcat gaatgctggt
     4621 cgcaggtggt gaggttggcc cgttccggtc ggtatcgaaa ggcagcgcag gcgggccgcc
     4681 gaacggcgcg ctcatcagcg tgacacagcg gaaaacatcc ggccggatca aagcagccca
     4741 ggcggagaca ttggcgccgg agtcgtggcc aaataatccc tgtacatggc gatagcccag
     4801 cgcggacacc aggctgagtg cgtcgcggac caggttcaac atgcgaaacg atgtgtagtc
     4861 gccatcgtag ttgccatccc aaccgctggt gcggccgtag ccgcgctgat cgggtgcgac
     4921 cacgtgataa ccggccgctg ccagcggtac catcactttt cgccagctat aggccagttc
     4981 cggaaagccg tgcagcagca atagcaacgg cctattttgc tgctcataac ccgcttcgag
     5041 gatatgcatg cgcagaccat tcacgtcgtt gacaaagcgg gaccggatgc cgggtggcaa
     5101 gactttttgg ggcagggggg caaggtcggt cattgttttg cgccggttaa ggaaaaacgc
     5161 accataacaa aaagccgtcc gcccgtttga caccaaaacg cgccgcaatt ttttccaagc
     5221 cagccgatga ccggcagtgc taggatttca acaccatgtc ggcacacggc ttcacgcttt
     5281 tcgataccgc catcggtcgc tgcggcattg cctggagtga ccaaggcatc gttggtgttc
     5341 agctgccgga aacccgtgag cgtgaaacgc tcgggcgcct gctgaaacac tatcccgacg
     5401 ccacccaagg gacgccgcca cccgcaatcc agcatgcgct ggacaacatc atttccttgc
     5461 tggccggcga atcggccgat ctatcggagg ctgcgctcga catgcggcac gtgccgccgt
     5521 tccatcgccg cgtgtatgac gtcgcccgcg ccattgcacc gggcgagacc atttcgtatg
     5581 gcgagattgc acggcaaatt ggtgcggggg gttccgcccg cgcagttggc caagcactcg
     5641 gccgcaaccc gtttgcaatt gtggtgccgt gccaccgcgt tctggcagcg ggcggaaaaa
     5701 tgggcggatt ctccgccaat ggcggcattg gcaccaagct gcgcatgttg tctatcgaat
     5761 ccgcccaggt caatggccac gacacgatgt ttgaaggcga tggcaccttc gggttcgacc
     5821 cggttgtcgc ggtcgcgcac ttgcgtgaat cgagtgcatt catggcgggt ctgatcgatg
     5881 cagtggggcc attgcgcatg cgcctcaaaa gaacgccaag catttttgtc gccctggccg
     5941 aagccatcgt ctaccagcag cttaccggga aagcagcagc caccatttat gcgcgcgttt
     6001 gtgcgctttt cccgcgtgct cacgaagggc cgacgccgga aaagatctgg cgtgtttcac
     6061 cagaaaaatt gcgagctgcc ggactatcga atgcgaaact gcttgcgctg aaagatttgg
     6121 cgcgcaagag cctggacggc gatattccga cgctgagcca ggtccacggc atggaagacg
     6181 aggcgatcat tgagcggctc gtgaaagtcc gtggcatcgg ccgctggacc gtcgaaatgc
     6241 tgttgatttc ccggttgggg cggtccgaca tcctgccggt ggacgactat ggcgtgcgca
     6301 agggctacgc gcttgctttc cgcaagcgta atctgccggc acccaaggcg ctggccaagc
     6361 tcggcgtgca atggaagcct tatcgctctg ccgcgagctg gtatttttgg cgcgcgttgg
     6421 aactccccaa gaaatagagg gccagtaagt tcattacttg ctttgcttcg cgtgttcgtg
     6481 acttggtaaa ctttattgtc gccgggtggt ggaacatttg gtccggtacc caaaagcgcg
     6541 gccggttgtt gagtcgattg gtcagcccta aaatttccaa atgctgatca gaactgaatt
     6601 gaaaaaaggt ccggtatcga tcatcgacta ccgctgtacg gccggtccga ccgaaaagcc
     6661 attcaccgag gtgcatcaga gctgctcggt gtcttacgtg cgccagggca gtttcggcta
     6721 tcgcacgcgc gggaaagttt tcgagctggt gactgggtcc gtgctggtcg gcaaggcggg
     6781 agaagaattc acctgtacgc atgattacca tggttgcggc gacgaatgcc tggctttcca
     6841 tctcgacccg gaggcagcgg atgcaattgg ttggcggacc ggcaactggc aaagcaccgg
     6901 tctgccgccg ttacccgagc tgatggttat cggtgaactg gcccagacgg ccgccgacgg
     6961 caccagcaac gtaggcatcg atgaaattgg ctggttgttt ccgctgcgac tcgcacaggt
     7021 actctctgga cggctgcagg atccgcaaaa ggctggttca cgcgatcggc gccgtgcggt
     7081 cgaagcagca ctttggctcg agacgcacgc gcatgaagag atcgacctgg aaggcgcggc
     7141 gcgtcaagcg gacatgagct cgttccactt cctgcgcgtg tttgccaaag tcatcggcgt
     7201 aactccgcac cagtatctgg tacgttcgcg tttgcgccat gcatcgcgtc tgctcgctgc
     7261 agacgaacgc ccgatcaccg acatcgcgtt cgatgttggc ttcgccgacc tctccaactt
     7321 cgtgcgcaca ttccatcgcg cggccggtgt ttcgccccgc gcattccgca aagctgcgaa
     7381 aggagagcgc aagattttcc aagatcggct ggctttgctg tcctaagctg ttctcctcgg
     7441 cacacggagg attgtcatgt acgaccacat cggcttgaaa gttaaagatc tggaagctgc
     7501 agtccgcttt tacgcggctg cgctcgctcc gctagggcac gttgtaggtc ccagcgacaa
     7561 gagttacgct ggaatcggcc cgaaaaatgc gcccggcctc tggttgcatg ccgcgagcgg
     7621 ctcgacaacc tcgggcacac atctcgcctt tcgcgcgtcg gaccgcgacg cggtcgatcg
     7681 gtttcatgcc gctggcctga aagcgggagg acgcgacaac ggaacgcccg gcttgcgcat
     7741 cgattacagc ccggactatt acgccgcgtt cctgttcgat ccggacggca ataacgtgga
     7801 ggcggtgttc atcaagtaac gttctccacg attggctggt tcactgaacc cgaagatggc
     7861 atgcgaccta tccagatacc gagacaacga tgacgaccat tcaagaaggc ggctgcgcgt
     7921 gcggcgatgt gcgttaccgg acagtcggcg accccgtatt ggcaacggtg tgtcattgca
     7981 agtactgtca aaagcgcacc gggagcgcgt tttccgaacc cgttatgttt cggattgagc
     8041 aagtggaatt ctcgggcacc gcgcgcacca tttatgagca tcaatctgac gaaagcggtc
     8101 gctggttgcg catggaattt tgccctcgtt gcgggaccac agtgtcctgg acggcccaac
     8161 gaaggcccgg gatgcgcggc atctcgggcg ggacgttcga caatcccgac tgggtaaaag
     8221 gccgcaaaca catctggacg cgctcggtgc agcagtggat gctcattcca gaggacgcag
//
LOCUS       DATMZQ010000151         8162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1849772, whole genome shotgun sequence.
ACCESSION   DATMZQ010000151 DATMZQ010000000
VERSION     DATMZQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849772"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VN496_04960"
     CDS             complement(<1..495)
                     /locus_tag="VN496_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16329.1"
                     /translation="MQRSLFQAVEQFFGPSLDAAATVIYWLSIVFLVHRLASPLLRLR
                     MELISARLKLHESVVMDCPYCHRETVVHDSQCAFCRKSLELPLSMRLWHFIRLRRQPI
                     WLHWIRWTWDSLGLAAFVALTVAGFIGLHAWSPVGSLQKLFIGVAVMCWVAISWLIGR
                     VMHLG"
     gene            665..1639
                     /gene="tal"
                     /locus_tag="VN496_04965"
     CDS             665..1639
                     /gene="tal"
                     /locus_tag="VN496_04965"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HXF16330.1"
                     /translation="MNLLESLKSYSVVVADTGDVDAIARLRPQDATTNPSLLFQSAQN
                     PRYRALVEDALAAGMRTRGDRKAKVDLVMDKLAVNFGCEILKHVKGRVSTEVNASLSF
                     DQEGSLAKAQQLIALYQQAGVAPERVLIKLASTWEGIRAAEKLERQGIHCNLTLLFSF
                     AQAVACADAGVTLISPFVGRIYDWYKKERKVEDIAAEEDPGVESVTRIYNYYKKFGYK
                     TQVMGASFRKVEQITGLAGCDLLTIAPDLMDKMEKTAGALTRRLSAESAKVSPAEKLS
                     VDEKTFRWLHNEDAMATDKLAEGIRRFHVDAGKLEQYVFALVEKSAAA"
     gene            1700..2101
                     /locus_tag="VN496_04970"
     CDS             1700..2101
                     /locus_tag="VN496_04970"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXF16331.1"
                     /translation="MSKKFVNPPGMKPLGMYSQVAVASGGAIAFISGQVAVDAQGRVV
                     GAGNIEAQAVQVFENLKLALAGIGATFEDVVKFTIFIVGLDQASRKAVMDVRGRYISR
                     ANPPAATMVGIDQLVQPELLVEIEAVVALEQ"
     gene            2182..2874
                     /locus_tag="VN496_04975"
     CDS             2182..2874
                     /locus_tag="VN496_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16332.1"
                     /translation="MPVLSRQMLTIMAMTVLVGLPLDRFGGTFGQSAVSAWTWVLLVI
                     LLMRVHQQLRSPLLLCLLIATLGESCLSLLWGLYVYRTHNIPLFVPPGHVVLFALGLT
                     LAPRVPRWGIRLITAFAATYALTAYATSTDTVSIALAILFLVFVAFGRNRQLYAMMFA
                     LSLVMELYGTWIGNWAWSPNVPGLPLTIANPPLCVGVLYCALDAGVVFANGLLRKWHR
                     PATAAASAVSAV"
     gene            2928..4073
                     /locus_tag="VN496_04980"
     CDS             2928..4073
                     /locus_tag="VN496_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXF16333.1"
                     /translation="MQYAMLRLFVLILLIHVYMGWRLLPDMPLGLAGMVTTGLLLATP
                     LCVLPFAEYARRHKQNPCADQLAGAGFLAMGFFSSLLLLTLLRDVLLLTSFAASGWLI
                     DPSTIGELRRWSAAAVPSMALVVTLVGFINARRRARIRTVDIPIDGLPVALHGFSIAQ
                     ITDIHVGATIKRGYVTRIVDAVNALEADMVAVTGDLVDGSVGRLAGHTEPMSRLTARH
                     GVFFVTGNHEYYSGVHAWIAELRRLGLSVLMNEHVVLVHRGESIVIAGVTDFNAHHFD
                     PSHRSDPAKAIVGAPEQAVKVLLAHQPRSAFAAEQAGYHLQLSGHTHGGQFFPWSLVI
                     KLLEPFSNGLYRLGKLSLYVSRGTGYWGPPKRFGAPSEITHLRLVPA"
     gene            complement(4049..5302)
                     /locus_tag="VN496_04985"
     CDS             complement(4049..5302)
                     /locus_tag="VN496_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXF16334.1"
                     /translation="MKVKTLETLSCDAGWRNYHFVKLTTDTGVVGWSEFDEGFGAPGV
                     GAVIARLAPRVIGQDDRAHERIYAELYSATRPGSGGVVGMAMGAIENALLDAKARELD
                     VPVYELLGGKIRDRIRVYWSHCATWRINHPAHYQPAITDLEGVKAIGREVRAKGFTAL
                     KTNIFTYDKQGTNARGWRPGFGVPFEPGLNVERDVLRNLVTHLQALREGAGPDVDILL
                     DLNFNAKAEGYLKILRAIAHLDLFWVEIDCFVPEALGYLRRHSPHPISSCETLLGLRE
                     FMPYFREQAMDVAIIDTPWNGVWQSMKIAAAAEAHEVNVAPHNFYGHLCTMMNAHFSA
                     AVPNLRIMETDIDRIAWDHELFDHVPEFVDGHLLMPQRPGWGIVPNEDAIRAHPPTSP
                     QGLLSYGRGNLPAGSRPAQAGTSRK"
     gene            5459..6628
                     /locus_tag="VN496_04990"
     CDS             5459..6628
                     /locus_tag="VN496_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16335.1"
                     /translation="MMKQARRRVLKLAGAVAATAAGVLAWRATDQGVFSAGSGAAYEP
                     WKQWDIRPPEGLLHPVYAAILAANPHNSQPWMFKVTEQTIDIYADTRRNIGAIDPQLR
                     EMYIGIGCALENLMIAAAHDGYATTLTFLPDRANPAHAVQVRLMRANPVTSDLYAAIP
                     RRHTNRGAYDISRALSAEMLQQLAARGAEFPEVKVLWFATQEERKRVGDLIVAATEAI
                     IADRQQSADSAKWFRTSWQQLQTQRDGITLDAQSLPPFINAAAKILPALDQQSADDAW
                     LRATRERHVATAAAFGFILVPDERNNIMRMLGGRLWQRLHLSATSQGLAAQPLNQMCE
                     RADREQQLGIEPRFGDALHALTADVGLRALMPFRIGYPTVSAPPSPRRDLKSVLV"
     gene            complement(6656..7540)
                     /locus_tag="VN496_04995"
     CDS             complement(6656..7540)
                     /locus_tag="VN496_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HXF16336.1"
                     /translation="MIRPSFPDTLIRVAVARFAVLALICAVLFGGCQSVQDIIGGAPK
                     PKAQVIGASIRGLSLENIVLLFDVEVENPYAANLPLADLHYSLASGSTHFADGSMQPT
                     GSIPARGKQVLQLPVTVQFASLLAALKGVKAGAVVPYTAEVKIGVDAPVLGRVDVPLS
                     KSGEIPVPAAPQVKLSSLAIGKLGFDQITASAKMQVKNTNQFALDLTKFGFLFALGGL
                     DVGSSKVSNPVKLPAGQTAIVEVPLSFSPRAAGVGIVNLLKSDQIAYQISGSIDANSR
                     FGPLTLPFSHIGNTKVTR"
     gene            7740..>8162
                     /locus_tag="VN496_05000"
     CDS             7740..>8162
                     /locus_tag="VN496_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXF16337.1"
                     /translation="MRIVTIVATAMLVLANPVLADDVSGTLMKIKDTGVIKLGFLSES
                     IPFSFAAADGKPMGYSTDLCTRVATGIQEQLGLPKLDIEWVPVTLETRFDAIKSGTID
                     LECGISSNTLSRQKQVDFSLMTWVDGANFLVRDDSPFKT"
BASE COUNT         1684 a         2329 c         2378 g         1771 t
ORIGIN      
        1 gcctagatgc atcacgcgcc caattagcca gctaatggcc acccagcaca ttactgcgac
       61 gccaataaac agtttttgca gagatccgac cggagaccat gcgtgcaacc cgatgaagcc
      121 cgcaacggtc agcgcaacga acgcggctag cccaagactg tcccatgtcc agcgaatcca
      181 gtgcagccag atcggttgtc gccgcaggcg tatgaaatgc cagaggcgca tgctcagggg
      241 caattcaagg cttttccggc aaaacgcaca ctgcgaatcg tgtacgacgg tttcgcgatg
      301 gcaataaggg caatccatga ccacgctttc gtggagcttc aggcgcgcgg aaatcagttc
      361 catgcgcagg cgcaataatg gggaggctag ccggtggacg aggaacacga tgctcaacca
      421 gtaaattacg gttgctgcgg cgtcgagcga tggaccgaag aattgttcga cggcctgaaa
      481 cagactacgc tgcatcagtg gaatccctgc ttcaattcgg tgaagccatt ataaatgtcg
      541 atgctgttgg aatgggtccc cacattcgaa ggattgtgtc tatgacgcca ttcgacgaca
      601 gcttcctgtg ccgtggtaag ttactgttat ccagcagtcc caatcaagat cacaggagtg
      661 aaccatgaat ttactggaat ccctcaaaag ttattcggtc gtcgttgccg ataccgggga
      721 cgtcgacgct atcgcgcggc tgcgtccaca ggatgcgacc accaatccgt cattgctttt
      781 tcagtcggcg cagaacccaa gatatcgcgc tctggttgaa gatgcgctcg cggcaggcat
      841 gcgcacacgc ggagacagaa aagccaaggt tgatctggta atggacaagc ttgcagtgaa
      901 tttcggctgc gagatcctga agcatgtcaa aggccgagtt tccaccgagg tcaatgcaag
      961 tttgtctttc gaccaggaag gcagccttgc caaagcgcag cagctgattg cgctgtacca
     1021 gcaggccggc gttgcgccag agcgggttct catcaagctg gcaagcactt gggaaggcat
     1081 ccgcgccgcg gaaaagctcg agcggcaggg catacattgc aacttgacgc tgttattcag
     1141 cttcgctcag gcggtcgcat gcgcggatgc cggcgtgacc ctgatatcgc cattcgtggg
     1201 tcgcatctac gattggtaca agaaggagcg caaggtcgag gacattgcgg ccgaggaaga
     1261 tccgggcgtt gaatcggtga cgcggatcta caactactac aagaaattcg gctacaaaac
     1321 gcaagtcatg ggtgccagct tccgaaaagt ggaacagatt accgggcttg cgggttgcga
     1381 tttgctgacc attgcgcccg atcttatgga caaaatggaa aaaaccgcag gtgcattgac
     1441 gcgccgacta tctgctgaat cggcgaaggt cagccccgcg gaaaagctgt ccgtcgatga
     1501 gaagacattc cgctggctgc acaatgagga cgccatggcg accgataaac tggcggaagg
     1561 cattcggcgt ttccatgtgg atgccggcaa gcttgaacaa tatgtatttg cactggtgga
     1621 gaaaagtgcc gctgcttgaa gcagctgtgc tgtgcgctcc aaataaacgc tgcggtcgca
     1681 aaccccttcg aggtaaaaaa tgagtaaaaa attcgttaat ccgccaggaa tgaagccgtt
     1741 gggtatgtac tcgcaagtgg ccgtggcgag cggcggagcc attgcgttta tcagcggtca
     1801 ggtggcagtc gacgcgcagg gcagggtggt tggcgcagga aacatcgagg cacaggcagt
     1861 acaagttttt gagaatctta aactggcgct tgccggcatt ggtgccacct tcgaagacgt
     1921 ggtcaagttc accatcttca ttgtcggcct ggatcaggcg agccgtaagg cggtaatgga
     1981 tgtgcgtggc cgatatatct cgcgcgcgaa tcctcccgct gcaacaatgg ttggcatcga
     2041 tcagctggtg cagccggaat tactggtgga aattgaagcg gttgtagcgc tggaacagta
     2101 aacctgtttc gcatgggatt cggcgccgga ttacgttttt tgcgcaggca ttggctgccc
     2161 gcgccgcgct tgctactgcc agtgccagtc ctttccaggc agatgctcac gatcatggcc
     2221 atgaccgtgt tggtcggttt gccgctggac cgtttcggcg gtacctttgg tcaatccgct
     2281 gtcagtgcat ggacatgggt actgttggtc atcctgctca tgcgcgttca ccagcagctg
     2341 cgttctccgc tgctgctttg tctgctgatc gcaaccctcg gcgaaagttg cttgtccctg
     2401 ctgtggggac tgtacgtcta ccggacacat aatattccgc tgtttgtgcc gcctgggcat
     2461 gtcgtgctgt tcgcattggg cctgacgctt gcaccgcgcg tgccacgctg gggcatcagg
     2521 ttgattactg catttgccgc cacatacgcg ctcactgctt atgcgacttc caccgataca
     2581 gtcagcatag cgctcgcaat cctttttttg gtattcgtag cattcgggcg caatcggcag
     2641 ctgtatgcaa tgatgtttgc cctgtccctt gtaatggagc tgtacggcac ctggatcgga
     2701 aattgggcat ggtcgcctaa cgtgcccggt ttgccgctga caattgccaa tccaccgttg
     2761 tgcgtcggcg tgctttattg cgccctggat gccggcgtag ttttcgccaa tggtctattg
     2821 cgtaaatggc atcgtccggc gaccgctgca gcatcagctg tatcggccgt ttagcttgtg
     2881 ctcgatgcaa tcgcccccat ttgaaaacga atctctcgcc cggatgactg cagtacgcca
     2941 tgcttcgatt attcgtcctt atcctcctta ttcacgtcta tatgggttgg cgtctgttgc
     3001 cggacatgcc gctggggctg gcgggcatgg tcacgaccgg gcttctgctg gccacacctc
     3061 tttgcgtgct gccgtttgct gagtacgcgc gtcgccataa acaaaatccg tgcgccgatc
     3121 agctggccgg ggcgggtttc ctggcgatgg gatttttctc ttcgctgctg ctcctgacac
     3181 tgttgcgcga cgtactgctg ctaacaagct ttgcagcaag cggatggttg atcgatccat
     3241 caaccatcgg ggagctgcgc cgctggtcag ccgcggctgt tccgtcgatg gccctggttg
     3301 tgaccttggt cggttttatc aatgcacggc ggcgcgcccg aatccggaca gtggacattc
     3361 ccatcgacgg ccttccagtg gctttgcatg gattcagtat tgcgcaaatc accgacatcc
     3421 atgtcggcgc cactatcaag cgcggctatg tgaccagaat tgtcgatgcg gtcaatgcgc
     3481 ttgaggctga catggtggct gtaacgggcg acctggttga cggcagcgtg gggcgcctcg
     3541 ccggacatac cgagcccatg agccggctga ccgcgcgcca cggggttttt ttcgttaccg
     3601 gaaaccacga gtattattcc ggagtacacg cctggatagc cgaactgcgc cgtcttggtc
     3661 tgtccgtgct catgaatgag cacgtcgtgc tggttcaccg tggcgagtcc attgtcatcg
     3721 ccggagtgac ggacttcaac gcccatcatt tcgatccgag ccaccgcagt gatccggcaa
     3781 aggcgatcgt cggcgctcca gagcaggcgg tcaaagtgct gcttgcgcat cagccgcgct
     3841 ctgcgtttgc tgcggagcag gccggttacc acctgcagtt gtccggtcac acccacggcg
     3901 gacaattctt tccatggagc ctggtcataa agctattgga gcctttcagc aacggactgt
     3961 accggctggg caagctctcg ctgtatgtca gccgcggcac gggttattgg ggcccgccaa
     4021 agcgattcgg tgcgccctcg gaaattactc atttgcgact ggtaccggcc tgagccggcc
     4081 gggagccggc gggcaagttg cctcggccgt agctcagcaa gccctgtggc gacgtcggcg
     4141 gatgcgcgcg aatcgcgtcc tcgtttggca cgatgcccca tcccgggcgc tgcggcataa
     4201 gcaaatgtcc gtctacaaat tccggcacat gatcaaacag ctcatggtcc caggcgatgc
     4261 ggtcgatgtc cgtttccatg atgcgcaagt ttggtaccgc cgcggaaaaa tgcgcattca
     4321 tcatcgtgca aagatgcccg tagaaattgt gcggggcgac gttcacttcg tgtgcctccg
     4381 cagctgccgc tattttcatc gactgccata cgccgttcca tggcgtgtct atgatggcga
     4441 catccattgc ctgttcgcgg aaataaggca tgaattcgcg taatcccagc aaggtttcgc
     4501 aggaactgat cggatgcgga ctgtgccggc gcaggtagcc caacgcctcg ggaacgaaac
     4561 agtcgatctc gacccagaac aggtccaggt gcgcgatggc gcgcaggatt ttgagatagc
     4621 cctcggcctt ggcattgaaa ttcaggtcca gcagaatgtc gacgtccgga cccgcacctt
     4681 cgcgcaacgc ctgcaggtgg gtcacgagat tgcgcagcac atcgcgctcc acatttaggc
     4741 cgggctcaaa aggcacgcca aagcccggtc gccagccgcg cgcattggtc ccctgcttgt
     4801 cgtaagtaaa aatattcgtc ttcagcgcgg tgaatccctt cgcgcgcact tcgcggccga
     4861 tcgcctttac gccctcaaga tccgtgatgg cgggctggta atgtgcggga tgattgatgc
     4921 gccaggtagc gcagtgcgac cagtacacgc gaattcggtc gcggattttg ccgcccagca
     4981 attcatagac cggcacatca agctcccgcg ccttcgcatc gagcagcgcg ttttcgattg
     5041 cgcccatcgc catgcccacg acgccgccgg atcccggacg cgtggcacta tagagttcgg
     5101 cgtagatgcg ctcatgtgca cggtcgtcct gcccgatgac acgcggagcc aaccttgcta
     5161 tgacggcgcc gactcccggt gcgccgaatc cttcatcgaa ttcactccag ccgacgacgc
     5221 ccgtgtcagt cgtgagtttg acgaagtgat agttgcgcca tcctgcatca caggacagcg
     5281 tctcaagggt tttgactttc atcgttgctt cctttcagcc tggtcggtcc gggtcggggt
     5341 ccgactgccc tggcgtgtcc gaattctagc cgagcattgt tcggcgttcg catccggtga
     5401 gcatatcgaa tcgggttaac attgcgtgga agcaatcgca aaaatttccg ggcatgccgt
     5461 gatgaaacag gcgagacgcc gcgttttgaa gttggcagga gccgttgcgg ccacagcagc
     5521 gggcgttctc gcgtggagag cgaccgacca gggagtcttc agcgccggaa gcggtgccgc
     5581 ctacgagccg tggaagcagt gggacatccg gccaccggaa gggctgctac acccggtgta
     5641 cgcagccatt cttgcggcca atccgcacaa ctctcagccg tggatgttca aggttacaga
     5701 acagacaata gacatttatg cggacacccg ccgcaatatc ggcgcaatcg atccgcaact
     5761 ccgcgaaatg tacattggta tcggttgtgc attggagaac ctgatgatcg cggccgcaca
     5821 cgatggctat gcaaccacac tgacattcct gcccgaccgt gcaaacccgg cacacgcggt
     5881 acaggtgcgg ctgatgcgcg cgaatccggt tacatcggac ttgtatgctg ccattccccg
     5941 gcggcacacc aaccgaggcg cgtacgatat ttcgcgcgcg ctctcagcgg aaatgctgca
     6001 gcaacttgcc gcacgcggtg cggaatttcc cgaagtaaag gtactgtggt tcgccactca
     6061 ggaagagcgc aagcgagtcg gcgatctcat cgtagctgcc actgaagcga tcatcgccga
     6121 ccggcagcaa agcgcggata gcgctaaatg gtttcgcacg agttggcagc agctgcaaac
     6181 gcagcgtgac ggcattacgc ttgatgccca aagcctgccg ccgttcatta acgctgccgc
     6241 caaaatcctg cctgcgctcg accagcaatc ggcggacgat gcctggttgc gggctacccg
     6301 cgaacgccat gtcgcgaccg cggccgcgtt cggattcata ctagtgccgg acgagcgcaa
     6361 caacatcatg cgcatgcttg gtgggcgctt gtggcagcgg ctgcatctgt ctgcgaccag
     6421 tcaaggattg gctgcacaac ccttgaacca gatgtgcgaa cgtgcagacc gggagcaaca
     6481 gctgggcatc gaaccgcgct ttggtgacgc tttgcatgcg ttaactgccg atgtcggcct
     6541 acgggcgctc atgccgttta gaatcggcta tccgaccgtt tcggccccgc cgagtccgcg
     6601 ccgcgatttg aaatccgtgc tggtctgaat gcgctgtcgg cgagcgctcg cagcatcaac
     6661 gtgtaacttt ggtattcccg atgtggctaa aaggcagcgt tagcgggcca aagcgggaat
     6721 ttgcgtcgat ggatccggag atctgataag cgatctggtc gcttttgagc aggttgacga
     6781 tgccgacgcc tgctgcgcgc ggcgaaaatg agagcggtac ttccacaatt gcagtctggc
     6841 ccgccggcag ctttacggga ttggaaactt tgctgctgcc gacatcgagc cccccaagcg
     6901 cgaacaggaa accgaatttc gtcagatcga gggcaaactg attggtattc ttgacctgca
     6961 tcttcgccga ggccgtgatc tggtcaaagc caagtttgcc aatggctagt gagcttaact
     7021 tgacttgcgg tgctgcgggc accgggatct ccccgctttt ggataatggc acatcgaccc
     7081 ggcccagcac tggcgcatcg accccgatct tgacttcagc cgtatacgga accacagcac
     7141 ccgccttcac gcccttgagc gcggccaaca atgaagcgaa ctgtaccgtg accggaagtt
     7201 ggaggacctg tttcccgcgc gccggtattg aaccagtcgg ttgcattgat ccgtcggcaa
     7261 agtgggtgct gccgctcgcc agggaatagt gaagatccgc caatggcaga ttggcggcgt
     7321 acggattctc cacctccaca tcgaagagca ggacgatatt ttccagtgac agcccgcgaa
     7381 tgcttgctcc gatgacctgg gctttcggtt ttggcgcacc cccgatgatg tcttgcaccg
     7441 actggcaacc gccgaagaga actgcgcaga tcagcgccaa aaccgcaaag cgagcgaccg
     7501 cgacgcgtat caaagtgtct ggaaaggatg gtcttatcat gctagcgaag agatagttgc
     7561 tatcgaatcg tggcgtccct tatgacaacg agcgccgccc ccatacgtgt cgcgagcctc
     7621 gagggttttt agcgacggac ctgaattgta ttctggaagt gtgtgctatc ctcgcgcccg
     7681 cagcataagt cccgccgcta ccaagcgttt ctcgtcaaat ccctttcctg gagagcaatg
     7741 tgcgaatcgt aactatcgtc gcaaccgcga tgcttgtgct ggctaatccc gtactggctg
     7801 atgacgtctc cggtaccctg atgaaaatca aggacaccgg tgtcattaag cttggctttt
     7861 tgtcagaatc aatcccgttt tcgttcgctg ctgcagatgg caagcctatg ggctattcca
     7921 ctgatctgtg cacccgcgtc gccaccggaa ttcaggagca actcggcttg ccgaagctcg
     7981 acatcgaatg ggtgccggtc acgctcgaaa cgcgctttga cgcgatcaag tcaggcacca
     8041 tagacctgga atgcggcatt agctccaata cgctttcccg tcaaaagcag gtcgacttca
     8101 gcctgatgac ctgggtggat ggcgcaaact tcctggtacg tgacgattct cctttcaaga
     8161 cg
//
LOCUS       DATMZQ010000152         4294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2134824, whole genome shotgun sequence.
ACCESSION   DATMZQ010000152 DATMZQ010000000
VERSION     DATMZQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134824"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VN496_05005"
     CDS             complement(<1..671)
                     /locus_tag="VN496_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF16338.1"
                     /translation="MKIVTLPSGEKVAALGQGTWYMGDKRGTRAEEIATLQLGLDLGL
                     TLIDTAEMYGEGKAEEVVGEAIAGHREQVFLVSKVYPHNASRKGAVAACERSLKRLHT
                     DRIDLYLLHWRGNIAFGETLEAFAALLKSGKIRYFGVSNLDLDDMRELTSLPGGVHVA
                     TNQLLYNLTRRGIEWDLLPWMRERHIPVMAYSPIEQARLLGNPKLVDFARRHAMTPVQ
                     VALGW"
     gene            832..1239
                     /locus_tag="VN496_05010"
     CDS             832..1239
                     /locus_tag="VN496_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF16339.1"
                     /translation="MRARRREWTVSRWTLLAVAVLCLWAGIHYADLLKAAFARNPVPR
                     TAESIARGQLLFQQQCAICHGERGLGDGPVAASLKDKPEDLSKIARPPVFPDGIVVYR
                     IANGKNSMPAFKTVLSETQLWDLLNFIRSLRTS"
     gene            1388..1987
                     /locus_tag="VN496_05015"
     CDS             1388..1987
                     /locus_tag="VN496_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXF16340.1"
                     /translation="MADIPMYHEGSRRMQDRNSTRKLADRLVEVLARNQFTDEDRAYI
                     ESRPLFFLATADAQGRPDCSYKGGPPGFVRVVAPDTLVFPDYDGNGMYKSLGNVLVNP
                     AVGMLFIDFESPRRLRVNGRASLSADDPLVSQFVGAKLIVRVQAEAIFPNCPRYIHKS
                     TIVENSIYTPCEGQVPPVPGWKKQPAFREVLPPEPASGD"
     gene            2231..2914
                     /locus_tag="VN496_05020"
     CDS             2231..2914
                     /locus_tag="VN496_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16341.1"
                     /translation="MLAGATALALSGTALAAEQSRDTQSRDNQTQTQPNATKGKPEQR
                     DAQKQQSDTGSSQSSQSGSQSSGSQTSQSDQSGSQSSQSGSQSSGSQTSQSDQSGSQS
                     SQSSQTSQSDQSGNQTSQSEDSSNPAVEPAKSGTQSGAAETQTSEQSGTTSSQPSQSG
                     ASETNRSGQQSQSDSSPSGTSSSSSQAGAGGGGSDQELSAALKKCDSLSGNQKQKCIN
                     SAKKKAGQM"
     gene            complement(3029..3256)
                     /locus_tag="VN496_05025"
     CDS             complement(3029..3256)
                     /locus_tag="VN496_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HXF16342.1"
                     /translation="MAKSKDVAEPIYKIVEVIGTSAKSWEEAAMNAVAVAAKSLRDLR
                     IAEVVKMDMKVDDGKVVAFRTRVLLSFKYEA"
     gene            3452..3727
                     /locus_tag="VN496_05030"
     CDS             3452..3727
                     /locus_tag="VN496_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16343.1"
                     /translation="MDGYPTQTELLTCLTNIANVAGDTSNIPFRVDVIPLHGKPPMYS
                     VMIKSPAKDLLRRQIGQILSRPFALGATSFMLTGSEAVSLVGHAQLK"
     gene            3954..>4294
                     /locus_tag="VN496_05035"
     CDS             3954..>4294
                     /locus_tag="VN496_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXF16344.1"
                     /translation="MSETRDALVTRLKTEALKVRRNIWRAVRAGGGGHIGGSSSAADI
                     LTALYFYRLKIRPKEPNWPDRDRFVLSKGHANAALGAVLAQAGFIEDAVLDKFYAFES
                     PFGMHPDIKVDG"
BASE COUNT          930 a         1269 c         1193 g          902 t
ORIGIN      
        1 aaccagccca gcgcaacttg taccggcgtc atggcgtggc gccgcgcgaa atcgacgagt
       61 tttggattac cgagcaatcg tgcctgttcg atcggcgaat aggccatgac cgggatgtgc
      121 cgttcgcgca tccagggcag caaatcccat tcgataccgc gtcgcgtcag gttataaagc
      181 agctggttgg tagccacgtg tacgccaccc ggcagcgacg tcagttcccg catgtcgtcg
      241 agatcgaggt tgctgacacc gaagtaacgg atcttgccgc ttttgagcag cgcggcaaat
      301 gcctccagcg tttcgccaaa cgcgatattt ccacgccagt gcagaaggta gagatcgatg
      361 cggtcggtgt gcaatctctt caagctgcgt tcgcatgccg cgacggcacc tttgcgggaa
      421 gcgttgtggg gatacacttt gctgaccaaa aaaacctgct cgcggtggcc cgcgatagct
      481 tccccgacca cttcctcggc tttgccctct ccgtacattt cggccgtgtc gatgagcgtg
      541 aggcccagat cgaggcccag ctgcaacgtg gctatttcct cggcccgcgt gccacgcttg
      601 tcgcccatgt accacgtgcc ttgcccgagt gccgcgactt tttctccgga aggcaatgta
      661 acaattttca atttggtttc cttgttcgta gcgttacgta aatattttat cagcgcgcca
      721 gaaactttca gattcagccc aacctagccg gtaagcggca ttttggtaaa tttcgattgt
      781 ccctgtccgg gcagcgtgat aattttttgc ctcctgcaat ccggtagacc tatgcgcgct
      841 cgtcggcggg aatggaccgt atccagatgg acactattgg cagtcgcggt attgtgcctg
      901 tgggccggta ttcattacgc cgatctgttg aaggccgcat tcgcgcgcaa tccggttccg
      961 cgcaccgccg agtcgattgc gcggggacaa ttgctgttcc agcagcaatg cgctatttgc
     1021 catggcgaac gcgggcttgg cgacgggccg gttgccgcat ccctgaagga taagcctgag
     1081 gacctcagca aaatcgcgag gccgccagtc tttcccgatg gtatcgtggt ctaccggatc
     1141 gcgaacggca aaaattcgat gccggctttc aagacggtgt tgagcgaaac gcaactctgg
     1201 gacctgctta atttcattcg ctccttgcga acgagctagc gctgcggtgg catggcatgt
     1261 tgtttacgac cgttacgttc gtcacgtccg ttatggtcga attaccctag cgaagctcgg
     1321 agtacccttt tagaccggta tcctgcgacg cagggcacga tcgtcacggt cttaaggaga
     1381 gatatctatg gctgacattc cgatgtatca cgaaggttct cgacggatgc aggatcgtaa
     1441 ttccacgcga aagctggccg accggctggt cgaagtgctg gcgcgcaatc agtttaccga
     1501 cgaggatcgg gcctacatcg agagtcgtcc gttgttcttc ctggcgacgg ccgatgcgca
     1561 aggaaggccg gattgctcgt acaaaggcgg cccgcctggc ttcgtgcgcg ttgtggcgcc
     1621 ggacacgctg gtctttccgg actacgatgg caatggcatg tacaaaagtc tcggcaatgt
     1681 gctggtcaat ccggcggtcg ggatgctgtt catcgatttc gagtctccac ggcgtttgcg
     1741 cgtgaatggc cgggcgagct tgagtgcaga cgatccgttg gtctcccagt ttgtcggcgc
     1801 caaactcatt gtgcgtgtgc aggcggaagc gatttttccg aactgtccgc gctatatcca
     1861 caagtcgacg attgtggaaa actccatata cacgccctgc gaaggtcagg tgcctcccgt
     1921 accgggctgg aaaaagcagc ccgcattccg cgaggttctg ccgccagaac cggcatccgg
     1981 tgattaacgc cggcacctcg atgcatttat taccagcgcg ctgcaattct tgccagccgt
     2041 aaccaaagcg tctccccctt ccttctgaaa aacagcgagg tgcatccttc caaacgatgg
     2101 catgggtgtt gcgaccatag tgtccgttgc tacggtaagg aatgcgggct cggataaaag
     2161 ctagccggcg atccatctgt caactttcat caaccattga ggagagacca tgagcaaatt
     2221 gctaactgca ttgctggccg gcgcgactgc acttgcgctg agcggcacgg cgctggccgc
     2281 ggaacaatcc cgcgacacgc agagccgcga caaccagaca cagacccagc cgaatgcaac
     2341 caagggcaag cccgaacagc gcgatgccca gaaacaacaa agcgacaccg gcagctcgca
     2401 atcgagccag tccggatcgc aatcgtccgg atctcagacc agtcagtcgg accagtcagg
     2461 cagccaatcg agccagtccg gatcgcaatc gtccggatct cagaccagtc agtccgacca
     2521 gtcaggcagc caatcgagtc agtccagcca aaccagtcag tccgaccagt cgggaaacca
     2581 gacaagtcaa tcggaggatt cctcgaatcc cgccgttgag cctgccaagt caggaacgca
     2641 gtccggcgcc gccgaaaccc agaccagtga acaatcaggc accacgagca gccagcccag
     2701 ccagagcggc gcatcggaaa ccaatcggag tggccagcaa agccaaagcg attccagccc
     2761 gtcgggcacc agcagcagct ctagccaagc cggggcaggt ggtggtggga gcgatcagga
     2821 actcagcgcc gcgctgaaaa aatgcgactc gttgagcggc aaccagaaac aaaaatgcat
     2881 caattcggcc aagaaaaaag ccggccagat gtaagaacca gcaagtctga agtagtaaac
     2941 gggcgccacg ggcgcccgtt tacattgcga ggaccggcga gtcgatgcca cgtactgtca
     3001 attgcaacgg accgctgcaa ttacggcgtc aggcttcgta cttgaacgac agcagcaccc
     3061 gggttcggaa cgcgacgacc ttgccgtcat cgaccttcat atccattttg actacttccg
     3121 cgatgcgcag gtcgcgcagg ctctttgctg caactgcgac cgcgttcatg gcggcttctt
     3181 cccacgattt ggcactggta ccgatgactt cgacaatttt gtatatgggc tcagcaacat
     3241 ccttactctt ggccatggtt cgctcctcca tcaggttgtg tcaagcaaac gaaatcccca
     3301 ttggatcgta ctcctgattc tgtttccggt tccgggccca ggatcccagc gtactgcacg
     3361 gcactaaact cgggttatca tgtcaaaatc gtcaacggca ccggcagccg cccttttagt
     3421 tgagctgcct catcgaccca gaccctggct tatggatgga tatccgactc agaccgaact
     3481 tcttacctgc ctcacgaaca ttgccaatgt cgctggcgat acgtcaaaca taccgtttcg
     3541 cgtagacgtc attccattac atggcaagcc gccgatgtat tccgtcatga tcaaatcgcc
     3601 cgccaaagat ttgctgcgcc gtcagattgg ccagatcctc tcccggccct tcgcattggg
     3661 agcaacgtca ttcatgctga ccgggagcga ggcggtatcg ctggtcggac acgctcagct
     3721 caagtaaagc cgaaacgcgg ttagcgccgc gcttgagtcc agggcaccgt gcggtgccct
     3781 ttttgcgtgg caccatccat aggtgccgcg gcaagtacga gcctaggctt cggtcctgcc
     3841 cgcaaagagc aacgcacagc gtgctttttc gcccttgttc gatttcggct accatttgct
     3901 cataacaata gcttttcgga ggagcacacg gcgtctagcc gtatgcgaaa gccatgagcg
     3961 aaacccgtga tgccctggtc acgcgattaa agaccgaagc cctgaaagtc aggcgcaata
     4021 tctggcgcgc tgttcgcgcc ggcgggggcg gacacattgg cggatccagt tcggcagccg
     4081 acatccttac ggcgctgtat ttttatcggc tcaaaattcg tcccaaggaa ccgaactggc
     4141 cggaccgtga ccgcttcgta ttgtcaaagg gacacgcgaa tgccgcgctt ggcgccgtgc
     4201 tggcacaggc cggattcatt gaagatgccg ttctcgacaa gttttatgct ttcgagtctc
     4261 ccttcggtat gcatcccgac atcaaagtgg acgg
//
LOCUS       DATMZQ010000153         9339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1161833, whole genome shotgun sequence.
ACCESSION   DATMZQ010000153 DATMZQ010000000
VERSION     DATMZQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161833"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VN496_05040"
     CDS             <1..263
                     /locus_tag="VN496_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXF16345.1"
                     /translation="IIAVVMTFSVLFTFTDFQLIWVLTRGGPINATHLMATLSFQRAI
                     PGGALGEGAAIATAMIPFLLAAILFSYFGLQRRRWQQGGSDK"
     gene            300..1169
                     /locus_tag="VN496_05045"
     CDS             300..1169
                     /locus_tag="VN496_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXF16346.1"
                     /translation="MRKPEAREGMHYLESVTRRWVTVYIPLLVFLFVLLFPFYWMVIT
                     SFKSNAELLSTSGNPFWVVQPTLDHFRKLFFGTSYPKWMWNTVIISVTATFVSLAASV
                     FAAYAIERLRFRGSRQIGLAIFLAYLIPPSILFIPLAMMVFQVGLFDTRWALILTYPT
                     FLIPFCTWLLMGYFRSIPFELEECALIDGATRWQILIKIVLPLAVPGLISAGIFAFTL
                     SWNEFLYALTFISASENKTVPVGVITELVEGDVYHWGALMAGALVGSLPVAIMYSFFV
                     EYYVAGMTGAVKE"
     gene            1353..2876
                     /locus_tag="VN496_05050"
     CDS             1353..2876
                     /locus_tag="VN496_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HXF16347.1"
                     /translation="MLQAADLTIRLNPADDVVIARVELPAGTEIIKEKIRASVTVPAG
                     HKIATHDIAEGSPVRRYNQIIGFAVKPIKAGDHVHVHNLAMGDFQRDYAFCADYKPVD
                     YVPHPATFQGIVREDGRVATRNYLGVISSVNCSAHVCRTIAKFFDDDFMAQYPNVNGV
                     VPIHHKTGCGMGSEGEPVDLLRRVLAGYATHPNFHSSLMIGLGCEANQINNLLAAQGL
                     KRSRQLQAFTIQETGGTKKTVADGIARIKELLPDANKVKREPVPASHLTLGLQCGGSD
                     GYSGISANPALGAAVDILVRNGGTAILSETPEVYGAEHLLTRRAATRAIGEKLVQRIH
                     WWEDYTTRLGGEMNNNPSPGNKAGGLTTILEKSLGAVAKGGTQAMMGVYEYAERVSAK
                     GFVFMDTPGYDPVSATGQVAGGANMICFTTGRGSAYGCKPSPSLKLATNTAMFNKQED
                     DMDINCGVIVDGKASIQEVGQQFFEIILRTASGEPSKSEAMGYGEDEFAPWILGPTM"
     gene            2986..3264
                     /locus_tag="VN496_05055"
     CDS             2986..3264
                     /locus_tag="VN496_05055"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HXF16348.1"
                     /translation="MAEDPSKELFEFFQKMWNPMSFPIPGMFQPTMSVEEVQKKIREL
                     DAVENWLKMNLTFVQMTVKTLEMQKSALETIQESAKQAAPDKKPKTGK"
     gene            3470..3628
                     /locus_tag="VN496_05060"
     CDS             3470..3628
                     /locus_tag="VN496_05060"
                     /inference="COORDINATES: protein motif:HMM:NF023508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HXF16349.1"
                     /translation="MNVISKLLDSLDSYLARQEHRRVEAFLAQAQNLGELEYRLRLLD
                     SKSNHSLF"
     gene            3638..3844
                     /locus_tag="VN496_05065"
     CDS             3638..3844
                     /locus_tag="VN496_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16350.1"
                     /translation="MISAFRRLFDTRRPRIAVRAKVNLIGTFGVLERTDGVVESVVGD
                     EAYVEWANGGRSVESTRHLVQITG"
     gene            3981..4919
                     /locus_tag="VN496_05070"
     CDS             3981..4919
                     /locus_tag="VN496_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXF16351.1"
                     /translation="MAKGSAFTSAPDLPPHIRQHFPFASRFIAVNGHRMHYVDEGEGD
                     PVLLLHGNPTWSFLYRKFIPPIRAAGYRVIVPDHIGFGLSEHPAREHDFSLENHIANL
                     AEFVRRLDLKRLTVVCQDWGGPMGLSCAVLDPASFKALVVMNTWAWPEPSTFHSSIFP
                     WRMMHAPVVAPYLFQRRNVLVERGLYLSVVHRERFIEEALPAYRFVMPDYDSRLLTRV
                     FPRLIPLGHDDRSAATMRWLEKSLGQTNIPALIVWGREEIVFPAGCAERFKKLLPHAR
                     GPLWVTGSHFLQEDSPQEICGHILEFLREIDRESQP"
     gene            4916..5317
                     /locus_tag="VN496_05075"
     CDS             4916..5317
                     /locus_tag="VN496_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16352.1"
                     /translation="MNIEIITAAELRTKSYRNVFVMVEERLGHYDGFREAIVGAFGLT
                     PAHADGGAPGYFRAPSGREYQIIFMARSGQRFPSGIEIHALVPQFDAVDPEAADEDIW
                     HFLEWLIARVGGEWSVEALKATGKVYKIPWA"
     gene            complement(5353..6180)
                     /locus_tag="VN496_05080"
     CDS             complement(5353..6180)
                     /locus_tag="VN496_05080"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXF16353.1"
                     /translation="MNEQVESLSHIKATVIDLALRFGPKVLVAIIILVVGVFVGRWIG
                     KAMARGLRKFDLEIPVRDLLVRVVNILVMGLFAIMALQNLGVELLPLIAGIGVAGAGI
                     ALATQGVLSNVVAGLTIIFTRPFRVGEYIAIAGVEGGVESIGLFKTVLTHPDLSKVVV
                     PNRKIVGEILHNYGNIRQLTITVGVSYDTNLNKALATVSEILQNNPRIFTEPAAGIGV
                     SVLADSSIQLAIKPWVKVADFGPAASEINMAIVEAFRNRNIVIPFPQQEVRMLGNAA"
     gene            complement(6287..7261)
                     /gene="pip"
                     /locus_tag="VN496_05085"
     CDS             complement(6287..7261)
                     /gene="pip"
                     /locus_tag="VN496_05085"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HXF16354.1"
                     /translation="MDFSPPQIRTELYPEIEPSRFSHLRLDDRYEMYWEESGNPQGAP
                     VVFLHGGPGAGASPMHRRFFDPAHYRIIIFDQRGAGRSRPHGDLIDNTTPHLIADMEK
                     LRVHLGIEKWVVFGGSWGSTLGLAYAQEYPQRCSALVLRGIFLCRKLEIDWFLYGMRF
                     IFPEAWRRFAEYIPEAERTNLLAAYTRRLNNPDPLTHMPAAHQWSIYEGSCSTMLPNA
                     SMVAHYSENAVALGLARIEAHYFANNIFLPENSLLNNAYRLRLIPGVIVQGRYDMVCP
                     IISAHDLHAAWPEAEYIVVPDAGHSAWEPGISAQLVAAMERIKARTRG"
     gene            7428..>9339
                     /locus_tag="VN496_05090"
     CDS             7428..>9339
                     /locus_tag="VN496_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXF16355.1"
                     /translation="MDELLRKSALDYHRLPTPGKISVVPTKALLNQRDLALAYSPGVA
                     AACEEIVRDPTTARNFTARGNLIGVITNGTAVLGLGPIGPLASKPVMEGKGVLFKKFA
                     GIDVFDIEINERDPDKLVDIIASLEPTFGGINLEDIKAPECFYIEQQLRSRMKIPVFH
                     DDQHGTAIIVGAAVLNGLKVVGKDITKVKLVCSGAGAAALACLDLLVSLGLSMENIWV
                     ADIKGVVYSGRKEEMDDNKARYAQHTKARTLAELLPDADIFLGLSAARVLKPEMVKHM
                     GARPLILALANPEPEILPNEAKAVRPDAIIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTITEQMKLAAVRAIAELAQAEQSDIVAIAYGEQELAFGPEYLIPKPFDPRLIEKIA
                     PAVALAAMKSGVATRPIQDFIAYHDQLSQFVYHSGLIMKPVFSAAKKNPTRVVFCEGE
                     DERVLRSVQIVVDEGVAKPILIARPEVLLPRMEKFGLRIRPGRDFEIVNPNSDPRFRD
                     YWQEYYTLVQRKGISVEYAKREVTRRTTLIGALMVRRGDADAMLCGTIAQFSLHVEYI
                     ATVIGLRKGVNQFAAMNMLILPNRTLFICDTYVNPDPTADQIAEITMMAADEVLRFGI
                     TPKIALLSHSNFG"
BASE COUNT         1980 a         2654 c         2700 g         2005 t
ORIGIN      
        1 cgatcatcgc ggtggtcatg acgttctcgg tgctgtttac gtttaccgat tttcagttga
       61 tctgggtgct aacgcgaggc ggccccatca atgcgacgca tttgatggcg actttgtcgt
      121 tccagcgcgc gattcccggc ggtgcactgg gggaaggggc ggcaatcgcg accgcgatga
      181 ttccgttcct gcttgcggca atcctgttca gttattttgg cctgcagcgc aggcgatggc
      241 agcagggcgg aagcgacaaa tgagaccagt acccgatcgc ctggttcaag tcatgcgcaa
      301 tgcgtaaacc agaagcccgt gaaggcatgc actacctgga atctgttacc cgacgctggg
      361 tgaccgtcta cattccattg ctcgtttttc tgttcgtgct gctgtttccg ttttactgga
      421 tggtgattac ctcgtttaaa tccaatgccg agctgttgtc gacaagcggc aatccattct
      481 gggtagtcca acccacactc gatcatttcc gcaagctgtt ctttggtacg tcgtatccga
      541 agtggatgtg gaatacggta attatttcgg tgacagccac gtttgtttcg ctcgccgcaa
      601 gcgtatttgc cgcctatgcg atagagcgcc tgcgcttccg gggttcacgc cagataggcc
      661 tggctatttt ccttgcttac ctgattccac catcgatcct gttcatccca ctggcgatga
      721 tggttttcca ggttggtctg ttcgatacgc gctgggcgct gattctgact taccctactt
      781 tcctgatccc gttctgcact tggctgctga tgggttactt ccgctccatt ccgttcgaac
      841 tggaggaatg cgcgttgatt gacggcgcca cgcgctggca gatcctgatc aagatcgtgc
      901 ttccgctcgc agtcccggga ttgatttcgg ccggaatctt tgcgttcacg ttgtcgtgga
      961 atgaattcct ctatgcgctc acgttcattt ctgcgtccga gaacaagacg gtcccggtgg
     1021 gcgtcatcac cgagctggtg gaaggcgatg tctatcattg gggcgccttg atggctggcg
     1081 cgctggtcgg ctcactgcca gtcgcaatca tgtattcctt ctttgtcgaa tactatgtgg
     1141 ctggcatgac aggtgccgtg aaagaataga accagcatga aaagcacagg ctggcgtgaa
     1201 aaccaagccg cacgacagct ttggacgatg gagctactgt cgcccggaac cgcgactccc
     1261 cacaaaacgc gcacagcggc ataaaatagc agtctgattc accggcgcgg ctccaacccg
     1321 tccatgccgg ccgcccaccg aggagaccac aaatgctgca agctgccgat ctgaccatcc
     1381 gtctcaaccc cgcggacgac gtagtcattg cccgggtaga gctgccggcc ggaaccgaga
     1441 tcatcaagga aaaaatccgc gcgtccgtga cagtgccggc cgggcacaaa attgccacgc
     1501 atgacatcgc agaaggttcc ccggtgcgcc gttacaacca gatcatcgga ttcgcagtca
     1561 aaccgatcaa ggccggcgat cacgtgcatg tgcataattt ggccatgggt gatttccagc
     1621 gcgattatgc gttttgcgcg gattacaagc cggtggatta cgtgccccat ccggcgacgt
     1681 tccagggaat cgtgcgcgaa gatggacgag tcgccacccg taactacctg ggcgtgatct
     1741 caagcgtcaa ctgctccgcg catgtgtgcc gcacgatcgc caagttcttc gacgacgatt
     1801 tcatggcgca gtatcccaac gtaaacggcg tggtgcccat acatcacaag accggttgcg
     1861 gcatgggttc cgaaggcgaa ccggtggacc tgctgcgccg ggtcctggcg ggctatgcaa
     1921 cccatcccaa ttttcattcc tcgctgatga ttggcctggg atgcgaagcg aaccagatca
     1981 ataatctgct ggccgcgcag ggactgaagc gttcacggca attgcaggcc ttcaccatcc
     2041 aggaaaccgg cggcaccaag aagactgtcg ccgacggcat tgcgcgcatc aaggaattgc
     2101 tgccggatgc caataaagta aaacgggaac cagtccctgc gtctcacctg acgctgggac
     2161 tccagtgcgg gggatcggac ggttattcgg gcatttccgc caatcctgca ttgggcgccg
     2221 cggtggatat tctcgtgcgc aatggcggca ccgcaattct ttcggagacg cccgaggtct
     2281 acggcgcaga gcacctgctg acccggcgcg cggcaacacg cgcaatcggt gagaagctgg
     2341 tccagcgcat tcactggtgg gaggattaca ccacccgcct tggtggagag atgaacaaca
     2401 atccatcgcc gggcaacaag gccggcggat tgaccacaat tttggagaag tcactcggcg
     2461 cggtcgctaa aggcggtacc caggccatga tgggggtcta tgaatatgcc gaacgtgtca
     2521 gcgcgaaggg tttcgtgttc atggataccc cgggctacga ccctgtctca gctaccggcc
     2581 aggttgcggg cggtgcgaac atgatttgct ttacgaccgg gcgcggttcc gcttacggct
     2641 gcaaaccatc gccatccctg aaattggcga caaacacggc aatgttcaac aagcaggaag
     2701 atgatatgga catcaactgc ggcgtaatcg ttgatggcaa ggcgagcatc caggaagtcg
     2761 gtcagcagtt tttcgaaatc attttgcgca cggcgtccgg cgaaccaagc aagagcgaag
     2821 caatgggata cggcgaggat gagttcgcgc cctggatact tggcccgacg atgtaagcgt
     2881 ggggacgacg agatggatac agcagtgcgc atcttgctcg gctaaccagt tttaacgagg
     2941 tcacgccgcg cgattttcgg atcgtcgcgg aagcggagcg aagcaatggc agaagatcca
     3001 tccaaagagt tgttcgagtt cttccagaaa atgtggaacc cgatgtcctt tcccattccg
     3061 ggaatgttcc agccgaccat gagcgtcgag gaagtgcaga aaaaaatccg cgagctcgat
     3121 gcggtcgaaa actggctgaa gatgaatctg acgtttgtcc agatgaccgt caagacgctg
     3181 gaaatgcaga agtccgcgct ggagaccatc caggaatccg ccaagcaggc tgcgccggac
     3241 aaaaagccca agacgggtaa ataagcttac ggctttacgg tcgccgggcc tggcgacagt
     3301 tgcagctccc tatccggacc ttgcgcgacg ctggccgcgt cgttcagttc ctcccgattc
     3361 ccctgtttta atgcatagca gcaatgcttt tcagcgcgtt aatttggcgc ctttaagcct
     3421 cataatgatg cagtgcaata atgcgtttcg tggctgacag gaggccgaaa tgaacgtaat
     3481 tagcaaactg ctggattcgt tagacagtta ccttgcaagg caagagcatc gtcgggttga
     3541 ggcgttcctt gcgcaagcgc agaaccttgg cgagcttgag taccggctgc ggctactgga
     3601 ctcaaagtca aatcactcct tgttttgagg agctgccatg atctctgcct tccgtcgcct
     3661 gtttgacacg cgccgcccgc gcatagctgt gcgggccaag gttaatctga tcggtacatt
     3721 cggggtattg gagcggaccg acggggtagt cgagtccgtc gtcggggacg aagcctatgt
     3781 tgaatgggcg aatggcgggc gatccgtcga aagcacgcgc catctggtgc agatcaccgg
     3841 ctgacctcac cacaaggcgg cattcgccgc gccggccttt cgccggttcg tagatctccc
     3901 actcgcggag aaggttcacg cgccgatccg tggcaaactg cgcatccgaa tcatgcaacc
     3961 ttcgatgcgc agctcactca atggccaagg gttctgcttt tacgtcggcg cctgacctgc
     4021 cgccgcacat tcgtcagcat ttccccttcg caagccggtt catcgcagtg aacggccatc
     4081 ggatgcacta cgtcgatgag ggcgaagggg atccggtatt gctgttgcac ggcaatccga
     4141 cctggtcatt cctgtatcgc aaattcattc caccgatcag ggcagccggt taccgggtca
     4201 tcgtgccgga tcacattggc ttcgggctat ccgagcatcc cgcgcgcgaa cacgatttca
     4261 gcctggagaa tcacatcgcg aatctcgctg aattcgtccg ccggctggat ttgaagcgac
     4321 tgacggttgt ctgtcaggac tggggcggcc cgatgggact ctcgtgtgca gtgctcgatc
     4381 cggcctcctt caaggcgctt gtggtgatga atacgtgggc gtggccggag ccaagcactt
     4441 tccatagttc catttttcca tggcgcatga tgcacgcgcc cgtggtagcg ccttacctgt
     4501 tccagcggcg caatgtcctg gtggagcgcg gactttatct ttcagtggta caccgtgagc
     4561 gcttcatcga ggaggcgctg cctgcctacc ggtttgtcat gcccgactac gacagtcgcc
     4621 tgctcacgcg cgtctttccg cgacttatac cccttggcca cgatgatcgt tctgcggcga
     4681 ccatgcgctg gctggaaaag tctcttggcc aaaccaatat tccggctttg atcgtctggg
     4741 gcagggagga aatcgtgttt cctgccggct gcgccgaacg cttcaagaag ttgctgccgc
     4801 atgcgagagg tccattgtgg gtcactggct cgcatttcct gcaggaagat tcgccgcagg
     4861 agatctgcgg ccacatcctg gaatttttgc gcgagattga ccgggagagc cagccgtgaa
     4921 catcgagatc atcactgccg cggaactccg cacgaaaagc taccggaatg tttttgtgat
     4981 ggtggaagaa cggcttggcc actacgatgg ttttcgcgaa gccattgtcg gcgcattcgg
     5041 ccttacgccc gcgcatgccg atggtggcgc accgggttat ttccgcgccc cgtcagggcg
     5101 cgaataccag atcatcttca tggcgcgctc cggccagcgc tttccgtccg ggatcgagat
     5161 ccacgcgctg gtcccgcaat ttgacgcggt tgaccccgaa gcggccgacg aagatatctg
     5221 gcatttcctc gagtggctaa tcgctcgcgt gggcggggaa tggtcggttg aagcgctcaa
     5281 ggcgaccggc aaggtttaca agattccctg ggcctgatcc tcgacagtgt tggttgaatg
     5341 gccgccgcgc catcaagcgg cgttgccaag catcctgacc tcctgctgcg ggaacgggat
     5401 gacgatattc cggttgcgga acgcttccac gatggccatg ttgatttcac tcgcggcagg
     5461 cccgaaatca gccaccttta cccagggttt tatcgcaagc tgaattgaag aatccgcgag
     5521 cacgctgacg ccgatgcccg ccgccggttc tgtaaatatg cgtggattgt tctgcaggat
     5581 ttcgctgacc gtggcgagcg ccttgttcaa gtttgtgtcg tacgaaacac ccaccgtgat
     5641 cgtcagctga cggatgttgc cgtagttgtg cagaatctcg ccaacgatct tgcggttcgg
     5701 gacgaccact ttggacaagt cggggtgagt cagtacggtt ttgaacagcc cgatggattc
     5761 gacgccacct tcgacgccgg caatggcgat gtattcaccg acacggaatg ggcgtgtgaa
     5821 tatgattgtc agccccgcca cgacgttgct caatacgcct tgcgtggcga gcgcaatgcc
     5881 cgcgccggcc acgccgatac cggcgatcag cggcaatagt tccacgccga gattctgcag
     5941 ggccatgatc gcgaataagc ccatgacgag tatattgacg acccgcacca atagatcgcg
     6001 gactggaatc tccaggtcga atttccgcaa gccgcgcgcc attgctttgc cgatccagcg
     6061 tccgacaaac acgccgacga caaggatgat gatcgcaacc aggaccttcg gcccgaacct
     6121 cagggcaagg tcaatgactg ttgcctttat gtgactgagt gattcaactt gttcgttcat
     6181 gcgagcgccc cctgtttggt ttttgacggc aacgactcac ccctcgacgg gggctgcaaa
     6241 acacgtctga ataactgctg tcgtaaaagc acagggcgcg agtggttcag cccctggttc
     6301 ttgcctttat tcgttccatt gcggcaacca gctgggcgct tatgccgggc tcccacgccg
     6361 aatgtcccgc gtcgggaacg acaatgtatt cggcttccgg ccaggccgcg tgcagatcat
     6421 gcgccgaaat aatcggacaa accatgtcat agcggccttg gacaatcacg ccggggatca
     6481 ggcgcagccg gtacgcattg ttcagcaacg aattttccgg caggaaaatg ttattggcaa
     6541 agtaatgcgc ttcgatgcgt gcaagtccga gcgcgacggc gttttccgaa tagtgcgcca
     6601 ccatcgaagc attcggcagc atggtggaac aagagccttc gtaaatgctc cattgatgcg
     6661 cggccggcat gtgcgtgagc gggtcaggat tgttcagccg cctggtgtag gcggcaagca
     6721 ggtttgtccg ctccgcctcc ggaatgtatt ccgcgaatcg gcgccaggcc tccgggaaaa
     6781 tgaaacgcat gccgtacaag aaccaatcga tctccagctt gcggcacaga aaaatgccac
     6841 gcagcaccag cgcggagcag cgttgcggat attcctgcgc gtaggcaaga cccagcgtac
     6901 taccccaaga accgccaaag accacccatt tttcgatgcc gagatgcacg cggagttttt
     6961 ccatgtcggc aatcagatgc ggcgtcgtgt tgtcgatcag gtctccatgc ggccgcgagc
     7021 gaccggcccc gcgctggtca aatatgatga tccggtaatg cgccggatcg aagaatcgcc
     7081 ggtgcatcgg gctggccccg gccccggggc cgccgtgaag gaaaaccacg ggtgcgccct
     7141 gtggattgcc cgactcttcc cagtacattt cgtaccgatc gtccagtcgc agatgactga
     7201 agcgcgacgg ctcgatttca ggataaagct cggtgcggat ctgtggcggc gaaaagtcca
     7261 tatttctagg ctggcggatc gacttacggc atcaagctga cgccagtgga aacacaggaa
     7321 ccatagcaaa aagcccggcc tgcgacaaac ttcctggatt cgatgtcgct gaaagaccgg
     7381 gagatgcgtc ccgtataatc gatccaaaca tcaataggga gaaaaacatg gacgagctgc
     7441 tgcgcaaaag cgcgctcgat taccatcgct tgccaactcc gggaaaaatc tcggtcgttc
     7501 ccaccaaggc attgcttaac cagcgtgatc ttgcgcttgc atattcaccc ggagtcgcgg
     7561 cggcctgtga agaaatcgtg cgtgatccga ccacggcacg caacttcacc gcgcgcggca
     7621 acttgattgg cgtaattacc aatggcacgg ccgtactcgg cctgggaccc attggcccgc
     7681 tcgccagcaa accggtgatg gaaggcaagg gtgtgctgtt caaaaaattt gctggaatcg
     7741 atgtattcga tatcgaaatc aatgagcgtg acccggacaa actggtcgac atcattgcaa
     7801 gcctcgagcc tacctttggc ggcatcaatc tggaagacat caaggcgccg gaatgttttt
     7861 acatcgagca gcagttgcgc agccgcatga agatacccgt attccatgac gaccagcatg
     7921 gcactgcgat tatcgtgggg gccgccgtat tgaatggtct gaaggttgtc ggcaaggaca
     7981 tcaccaaagt aaagctggtg tgttcgggcg cgggggcagc cgcgctagcc tgtctggacc
     8041 tgctggtgag cctgggcttg tcgatggaaa acatctgggt tgcggacatc aagggcgttg
     8101 tttacagcgg gcgcaaggag gaaatggacg acaataaagc ccgctacgcg cagcacacca
     8161 aagcccgaac cctggcggaa cttctgccgg atgcggatat ctttctgggt ctctcggctg
     8221 cgcgtgtcct caagccggaa atggtgaaac acatgggcgc gaggccgctg atactcgcgc
     8281 ttgccaatcc cgagcctgaa attctaccga acgaggccaa ggctgtccgc cctgacgcca
     8341 tcatcgctac cggccgatcg gactatccga accaggtcaa caacgtgttg tgctttccgt
     8401 tcatttttcg tggcgcgctg gatgtcggcg ccaccactat taccgagcag atgaaacttg
     8461 cggccgttcg cgccattgcc gaattggcgc aggcggagca gtcggacatt gtcgcaattg
     8521 cctacggcga acaggagctg gcctttggtc ccgaatatct gattcccaaa cctttcgacc
     8581 ctcgcctgat tgaaaaaatc gcaccggccg tggcattggc cgccatgaaa agcggcgtgg
     8641 cgacgcggcc gatccaggac tttatcgctt accacgatca actctcgcaa tttgtctacc
     8701 actccggcct gatcatgaag ccggtattca gcgcggccaa aaagaatccc acgcgcgtag
     8761 tgttctgtga aggcgaagac gagcgcgtgc tgcgctcggt tcagatcgtg gtcgacgaag
     8821 gcgtagccaa accgatcttg attgcgcgcc cggaagtgct gctaccgcgc atggaaaaat
     8881 ttggtttgcg catccgtccg ggaagggact tcgagatcgt gaatccgaac tcggatccgc
     8941 gctttcgcga ttactggcag gagtactaca cgctggtcca gcgcaagggt atttcagttg
     9001 aatatgccaa gcgcgaagtc accaggcgga ctacgctgat cggagcgcta atggttcgcc
     9061 ggggcgatgc ggacgcaatg ttgtgcggaa ccatcgcaca gttttcgctg catgtggaat
     9121 acatcgcgac tgtcatcggg ctacgcaagg gggtgaacca gttcgcggcc atgaacatgc
     9181 tgatccttcc caaccgtacc cttttcatct gcgataccta tgtcaatccg gatccgaccg
     9241 ctgaccagat cgccgaaatt accatgatgg ccgccgatga agtcttgcgt ttcggcatca
     9301 cgccgaagat tgcactgctt tctcattcca attttggca
//
LOCUS       DATMZQ010000154         3095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4207430, whole genome shotgun sequence.
ACCESSION   DATMZQ010000154 DATMZQ010000000
VERSION     DATMZQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207430"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..876)
                     /locus_tag="VN496_05095"
     CDS             complement(37..876)
                     /locus_tag="VN496_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HXF16356.1"
                     /translation="MRLGDQDASNNVEDRRGMGIGGGIGVGGLVIAVIAYFLGFDPST
                     VLNVAQQVAPQSDTRQAPTGAPGDAMGQFVAKVLGSTEVVWGRIFAQSNVQYRAPTLV
                     LFDNQVRSACGMAQSATGPFYCPGDEKLYIDLSFFHDMQTRFHTPGDFAEAYVIAHEV
                     GHHIQKLNGTFQKVEATRGRVSRAEANRISVRMELQADCLAGVWGYHAGTMNQLESGD
                     IAEALNAATAIGDDRLQQQTQGRVVPESFTHGSSAQRVRWFKRGMESGRPQDCNTFSS
                     GSL"
     gene            1125..2210
                     /locus_tag="VN496_05100"
     CDS             1125..2210
                     /locus_tag="VN496_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16357.1"
                     /translation="MTSPLLEVAWDKCLLQPAPNKQAEAALRRDVGAAPAWVRYFLSC
                     PWLPKAAIRLAVDNKLLVHLQFSTVDLIALAVSQENSCRYCYAATRMQLRMLGMSEER
                     MEQLEQRLASGDMEPKLAAPVRFARRMARASPLVTAEDLEPLRAAGYSEAEIYELAFS
                     SACIGFFNRISTIPALPPYSWEQLADRWFMRVLRPLVAPMIRRWRKRGEPASFAQPPQ
                     GPFSGLLLQFDGSPIGPVLASAIEDVWASPILDSRCKALMFAVIGRGIGSDRYSREIS
                     QILESEGLVGDEAAHILAHLSGPGLDAETCALVAFARDTIWYQADQIQRRARELRERL
                     SEAKFVEAIGVVALANTLCRLCAAMPP"
     gene            2207..2998
                     /locus_tag="VN496_05105"
     CDS             2207..2998
                     /locus_tag="VN496_05105"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXF16358.1"
                     /translation="MSLAFALLAMALAVAAGLIVWQRTRLRKLRAHIHATTLELERLQ
                     RSCALLAPTGVVERLFSDGPDSIAEHKVFTAMFTDLVGYTALSERLDPAALAGVLNGY
                     FELVSNSVVEHRGRVATFLGDGILIFFGVLEPNPWQCNDAVEAALAIRAGLVRYNAEL
                     ERNGLQRIAVGVGIHRGPGLAGLIGSRERREYTVVGPTLNLAARVQSLTREHHQDILL
                     TEAVRAELDPHFKLSAMPALPVKGIAEQVVTYAVLEGRDEATLKP"
BASE COUNT          564 a          936 c          963 g          632 t
ORIGIN      
        1 aagcagaatc gttgtaacca gtgtggcgat caggccctac agcgagccgc tggaaaacgt
       61 attgcagtcc tgcggtcggc cggattccat gccacgcttg aaccagcgca cgcgctgcgc
      121 ggatgacccg tgcgtgaacg actcgggcac gacgcggccc tgcgtctgct gctgcaggcg
      181 atcatcgccg atcgccgtgg ccgcgttcag cgcttcggcg atgtcgccgg attcgagctg
      241 attcatggtg ccggcgtgat agccccagac gccagcgagg caatccgcct gcaactccat
      301 gcgcaccgaa atccgattcg cttcggctcg cgaaacgcgc ccgcgcgtgg cctcgacttt
      361 ctggaacgta ccgtttaact tttgaatgtg gtgaccgacc tcgtgcgcga tcacataggc
      421 ctccgcgaaa tcgccgggcg tgtgaaagcg tgtttgcata tcgtggaaga aagaaagatc
      481 gatatagagc ttctcgtcgc cgggacaata gaacggtccc gtagccgatt gcgccatgcc
      541 gcaggcggag cgcacctgat tatcgaacag caccagcgtc ggtgcgcggt actggacatt
      601 cgattgcgcg aagattcggc cccagaccac ttcggtgctg cccagaactt tggcaacgaa
      661 ctgccccatg gcatccccgg gcgcgcctgt cggagcctgg cgcgtatcgc tctgcggggc
      721 aacctgctga gctacgttca gcacggtgct ggggtcgaag ccaaggaagt atgcaatgac
      781 ggcgatcacc agtccgccca caccaatgcc gccgccgatg cccatcccgc gtcggtcttc
      841 gacgttgttg ctcgcgtcct gatcgcccaa tctcatgttg actccttcgg cctgaaagtt
      901 acgacatcac ccggtatctt atcgttccat atggttgagg ccgtcacggc cattggcagg
      961 gccgtttacc ttgatcatgt cagcctatga cgcaccgttc cgtcccggcg atcataatgt
     1021 gcgaacgcag tgccggggct tgtgtctgtt tggtttcgta tcggagtcaa tatcggacgc
     1081 cgcgagtcgc gtcataatgc atctttgcga accggtagac ccccatgact agtccattgc
     1141 tcgaggtcgc ctgggacaaa tgcctgctcc agccggcccc caacaaacaa gccgaggctg
     1201 ccttgcgccg ggacgtgggt gccgctcccg cgtgggtgcg ctattttttg agttgcccgt
     1261 ggctgccgaa ggcggccatt cgtctggcgg tcgacaacaa gctgctggtg cacctccagt
     1321 tttccacggt ggacctgatt gcgctcgcgg tcagtcaaga gaattcgtgt cgttactgct
     1381 acgcggccac gcggatgcag ttgcgcatgc tcggcatgag cgaagagcgc atggaacaac
     1441 tcgagcagcg gctggcgagc ggcgacatgg aacccaagct ggccgcgccg gtacgttttg
     1501 cgcgccggat ggcgcgcgcc agcccgctgg tgacggcaga ggatcttgaa cccttgcgtg
     1561 ctgccggtta cagcgaagcc gaaatatatg agctcgcatt ttcgtctgcg tgcattggct
     1621 tcttcaatcg catttcgacc attccggcac tcccgccgta cagctgggaa caactcgccg
     1681 atcgctggtt catgcgtgtg ctgcggccgt tggtcgcccc gatgatcaga cgctggcgca
     1741 agcgcgggga acccgcctca tttgcacagc caccgcaggg accattctcc gggctgctgc
     1801 tgcaattcga cggctcgcca attgggcccg tgctggcctc ggccatcgaa gatgtgtggg
     1861 cttcacccat cctggacagc cgctgcaagg cgctgatgtt tgcagtgatc ggccgcggca
     1921 tcggatccga tcgctacagt cgggagatca gccagatcct cgaatccgaa ggcctggtcg
     1981 gcgacgaagc agcacacatc ctggcgcact tgagcggtcc tgggctggac gcggagacgt
     2041 gcgcgttggt ggcgttcgcg cgcgatacca tttggtatca agcggatcag atccagcgcc
     2101 gcgcacggga gttgcgcgag cggctgtcgg aagcgaaatt cgtcgaagcg ataggtgtag
     2161 ttgcgcttgc caatacgctt tgccggctgt gcgccgcaat gccgccgtga gcctcgcgtt
     2221 cgctctgctt gcgatggcgc tcgccgtcgc ggccgggctc atcgtctggc agcgaacgcg
     2281 gttacgcaaa ctgcgcgctc atatccatgc aacaacgctg gaactggaac gcttgcagcg
     2341 atcctgcgcg ttgcttgcgc ccactggcgt agttgaacgc ctgttttccg acggccccga
     2401 tagcattgcc gaacacaaag tctttacggc gatgtttact gatctggttg gttatacggc
     2461 gctgtccgaa cggctcgatc cggcagcgct ggctggtgtg ttgaacggct atttcgagct
     2521 tgtcagcaac agcgtggtcg aacatcgcgg acgcgtcgca acttttcttg gtgatggcat
     2581 cctgattttc ttcggcgtac ttgagcccaa tccctggcaa tgcaatgacg cagtggaagc
     2641 cgcgcttgcc atacgcgccg gactcgtgcg ctacaacgcg gagcttgagc gcaacggctt
     2701 gcagcgcatt gctgtgggcg taggcataca ccgcggcccg gggctggctg gcctgattgg
     2761 cagccgcgag cgccgcgagt acaccgtggt tggtccaacg ctgaatcttg cggcgcgcgt
     2821 gcagtcgctc acgcgcgagc accaccagga cattctgctc acggaagctg tgcgcgccga
     2881 actggatccg catttcaagc tgtcggcgat gccggcgcta ccggtaaagg gcattgccga
     2941 acaggtggtg acttatgcgg tgctagaggg cagggacgaa gcgacgttaa agccgtgacg
     3001 acgtgtccca caaggggata ccaggctaac gcccataacg gtcgtgacgg tcgtgacgat
     3061 cgtgacggtc gtgaatgtcg tgaatatcgt gaata
//
LOCUS       DATMZQ010000155         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_755639, whole genome shotgun sequence.
ACCESSION   DATMZQ010000155 DATMZQ010000000
VERSION     DATMZQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755639"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VN496_05110"
     CDS             complement(<1..714)
                     /locus_tag="VN496_05110"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXF16359.1"
                     /translation="MKLELKGVFPAPPTPVTEDGRIHEKALRALLDDNIVHGCGGFWM
                     AGSTGEGPVLNDEQRDVVAKISAETCKGRALVIMQVGSMTTASAVKAAKTARVAGCAA
                     VCCLPPLFFRTSERSIIDYYKAVAGAAGELPFFVYNLPQLTQIEFFPAMMEKLAREIP
                     TLKGLKHSAQDFTQIRAFADMGLTCFSGNGALPLPALTMGAVGTIDAPLCLAPWHYAQ
                     LFSAWEAGNMKLAQSLQDQV"
     gene            884..1903
                     /locus_tag="VN496_05115"
     CDS             884..1903
                     /locus_tag="VN496_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXF16360.1"
                     /translation="MAISRRDLIKYGAAVAGAAVLSRRSWADTAPINLVFSHHVPTTH
                     LIHGVAEKFADKVKERTKGTVTISIRPASQLFNLRTSAEALQLGTLDLCWTDLGTLGN
                     WQPQFGFVSLPFLFNNYDHVKKVLYGPIGQQVCKDAKDSLGVEILSLGASGFRVFLSK
                     KPIQKADDVRGLRLRVPEIPTWVEMAKALGANPTPIPAGEIYTALQTGVVDAFESPAD
                     YIMSSRNYEVAGHVTRTHHIFTEVSMMASARKMASLPPGVQTILREAAVEAVQKEMWE
                     ANLKAQETAWNDLAKLVKANPNPDIASFRAKMGPVITGFIGRAGPKGKALVEAVQAAG
                     TRLDR"
     gene            1916..2428
                     /locus_tag="VN496_05120"
     CDS             1916..2428
                     /locus_tag="VN496_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXF16361.1"
                     /translation="MNNRSAGVAEPFVGRLRASLQVVSVALLASYFVLVLLQVFFRYV
                     LNESLFWAEELVRGAMLWGIMLSAALVGAARGHIRVEVLELMLPPRGRRMVIWLANVL
                     TLAFSLILLWAGVQFVDRTWMQQSPVLDVPKWTVYLAIPVGAVLESLLMLLTWNRDNR
                     VAEELADRTL"
     gene            2425..3699
                     /locus_tag="VN496_05125"
     CDS             2425..3699
                     /locus_tag="VN496_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXF16362.1"
                     /translation="MSIGLMLALFSIFMVLGMPIAYCMGLAAILTVLADGQLPVLLLA
                     QQFYDSLDNFSLLAVPLFILAGELMSVAGITERLVGLSRALIGHLRGGLAQANILTNM
                     FMGAISGSALADLAAIGSMMIPAMKREGYKPAFAVAVTACAAMMAPIIPPSIIAVIYG
                     SVTGVSIGALFLGGVLPGVLAGMSMMVLTWFMAPGAGAGPSPKASAAEALVATRRAMP
                     ALIMPAIIIGGILSGAFTPTEAGAVAALYALIFGLIARKHGFASLYRNFSAAAETTAS
                     ALITLGGAALFSWVLSRAGVGQLALQAMLSITSDPKLAMLVLITFFFLLGTFLEPVPA
                     LIIVVPIMGPLIAHLGYDPVHFGIITIMLLVVGSVTPPVGVLAMVASRIAGISYNASL
                     AMLSPFIAAWMIVILLVAYVPDLVLWLPRLMK"
BASE COUNT          713 a         1159 c         1153 g          826 t
ORIGIN      
        1 gacctgatcc tgcaacgact gcgccagttt catattgccg gcttcccatg cactgaatag
       61 ttgcgcgtag tgccacggcg ccagacagag cggcgcatcg atcgtaccca ccgcacccat
      121 ggtgagcgct ggcagtggca acgccccgtt tccggaaaag caagtcaatc ccatgtcagc
      181 gaacgcacgg atctgcgtga agtcctgtgc agaatgcttg agccctttca atgtcggaat
      241 ctcacgcgca agcttttcca tcattgccgg gaagaattca atctgtgtca gttgcggcag
      301 gttgtaaacg aagaacggaa gctcgccggc cgcgcccgcc acggccttgt agtaatcgat
      361 gatggagcgt tcgctggtgc ggaagaatag cggcggcagg cagcacaccg ccgcacaacc
      421 ggccacacgc gccgtctttg cggctttcac cgcgctggcc gtcgtcatcg agcctacttg
      481 catgatgacc aacgcgcggc ctttgcaggt ttccgcggaa atctttgcca ccacgtcgcg
      541 ctgctcgtcg ttcagcaccg gcccttcgcc ggtgctcccc gccatccaga agccaccgca
      601 gccgtgaacg atgttgtcat cgagcagtgc tcgcaaggct ttctcatgga tgcggccgtc
      661 ctcggtgact ggcgtaggcg gagcaggaaa tacgcccttg agttcgagtt tcatcgtgaa
      721 tcccgtttgg tggcaaatga ggagggattc tatcagcgtg gagcgagcct tttcggctac
      781 actgcttttc agcgcagaac acaggtgctg gcatgcagcg cggcgcgctg cgtacacaat
      841 aagatgacat cgcgcggcgg tgtcgcaaac ggggagcatc acgatggcca tttccaggcg
      901 tgacctgatc aagtatggcg ccgcagttgc gggcgcagcg gtgttgtcgc ggcgatcgtg
      961 ggcggatacg gccccgatca acctcgtgtt ctcgcatcat gtgcccacca cgcatttgat
     1021 ccacggcgtg gccgaaaaat ttgccgataa ggtcaaggag cgtaccaagg gcacggtgac
     1081 catcagcatt cgtccggcct cacagctatt caatctgcgc acttcagccg aagccctgca
     1141 acttggcacc ctggacctgt gctggaccga tctcggcacg ctgggcaatt ggcaaccgca
     1201 gttcggtttt gtatcgctgc cgtttctgtt caacaactac gatcacgtga aaaaagtgtt
     1261 gtacggaccg attggccagc aagtctgcaa ggatgcaaaa gattcccttg gcgtagaaat
     1321 cctgtccctg ggcgcttcgg gatttcgcgt gttcctgtcc aagaaaccaa tccagaaagc
     1381 agatgacgtg cgcggactgc gcctgcgtgt gcccgaaatt ccgacctggg ttgaaatggc
     1441 caaggcgctc ggcgccaatc cgacgccgat tcccgccggg gagatctaca ctgctctgca
     1501 gactggcgtg gtggacgcgt ttgaatcgcc cgcagactac atcatgtcga gccgcaacta
     1561 tgaagtggca ggacatgtga cgcgcacaca tcatatcttc actgaagtgt cgatgatggc
     1621 gagcgccagg aaaatggctt cgctgccacc tggcgttcag acgatccttc gcgaagcggc
     1681 cgtcgaagcc gtgcagaaag aaatgtggga agccaatctc aaagcgcagg aaactgcctg
     1741 gaacgatctc gccaagctgg tgaaggcgaa cccgaatccg gacatcgcca gtttccgcgc
     1801 aaagatgggc ccggtcatca ccgggtttat cggccgggcc ggaccgaaag gaaaagcttt
     1861 agtcgaagcc gttcaggccg ccggcacaag gcttgaccgc tgacaacagg caaacatgaa
     1921 taaccggtcc gctggcgtgg ccgagccctt cgtcggccgt cttcgcgcgt ccctgcaagt
     1981 agtgtcggtg gcgctgctgg caagttactt cgtgctcgtg ctgttgcagg tgttcttccg
     2041 gtatgtactg aacgaatcgt tgttctgggc cgaggaattg gtgcgaggcg cgatgctctg
     2101 gggcatcatg ctcagcgccg cactggtcgg agcagcgcgg ggccacatcc gcgtcgaggt
     2161 ccttgaactg atgttgccgc cgcgcggccg gcgcatggtg atctggctgg caaacgtact
     2221 gacgcttgcc tttagcctga tcctgctatg ggcaggtgta cagtttgtcg accggacgtg
     2281 gatgcaacaa tccccggtac tagacgtgcc gaagtggacg gtatatctgg cgatcccggt
     2341 cggcgctgtg cttgaatcgc tgctgatgct actaacctgg aatcgagaca accgcgtcgc
     2401 tgaagaactc gcggatcgca cgctgtgagc attggcttga tgctcgcgct gttctccatc
     2461 ttcatggtgc tgggcatgcc gattgcctat tgcatgggtc tggccgccat cctcactgtg
     2521 ctggccgatg ggcagcttcc ggtactgctg ctcgcccagc agttctacga ttcactggat
     2581 aatttttcgc tgctcgcggt cccgctgttc atcctcgccg gcgaactcat gagcgtcgcc
     2641 ggcatcacgg agcgcctggt cggcttgtcg cgcgcgctga tcggccatct gcgcggcggg
     2701 cttgcgcagg ccaacatcct gaccaacatg ttcatgggtg ccatctcggg ctcggcgctc
     2761 gccgatctcg ccgcaatcgg ttcgatgatg atcccggcga tgaaacgcga aggctacaaa
     2821 ccggccttcg cggtggcggt cacggcttgc gccgccatga tggcgccgat cattccgccg
     2881 agcatcatcg cggtgatcta cggttcagtc actggcgtgt ccattggcgc gctgttcctc
     2941 ggtggcgtcc tgcctggcgt gctggctgga atgtcaatga tggtgctgac ctggttcatg
     3001 gcgccgggcg ccggggctgg tccatcgcca aaagcgtccg cggccgaagc gttggtggca
     3061 acgcgccgcg caatgcctgc actgatcatg ccggccatca tcatcggcgg catcctgtcc
     3121 ggcgcattta cgccgaccga ggccggagcc gttgcagcgc tctatgccct gattttcggc
     3181 ctgattgcgc gcaagcatgg atttgcctcg ctctatcgca atttttccgc ggcagcggag
     3241 acgaccgcca gcgcactgat tacgctcggc ggcgctgcct tgttcagctg ggtgctgagc
     3301 cgcgcaggcg tgggccagct tgcactgcaa gccatgctct cgatcacatc agatccaaag
     3361 ctcgccatgc tggtactgat cacgttcttt ttcctgctgg gcacgttcct cgagccggta
     3421 ccggctctca tcatcgtggt gccgatcatg ggtccgctga ttgcgcatct cggctacgac
     3481 ccggtgcact tcggcatcat caccatcatg ctgctcgtcg tcggctcggt gacgccgcca
     3541 gtcggtgtgc tcgccatggt tgcgagccgc atcgcgggca tcagctacaa cgcctcgctc
     3601 gcaatgctgt caccgttcat cgcggcatgg atgatcgtga ttttgctcgt ggcgtatgtt
     3661 cccgacttgg tgctctggct gccgcgatta atgaagtaag cacgggcagc gctcttttct
     3721 ggggaactaa caaaagcgga aattccaccg cgaagatatt cgtggtgaaa tcctttcgca
     3781 ccgagattca tgccaccagt aatgccgcgc caatagccat cactgcctga tcgcttttcc
     3841 ggggcgcgtt g
//
LOCUS       DATMZQ010000156        13209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3233403, whole genome shotgun sequence.
ACCESSION   DATMZQ010000156 DATMZQ010000000
VERSION     DATMZQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3233403"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VN496_05130"
     CDS             <1..910
                     /locus_tag="VN496_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXF16363.1"
                     /translation="RYWRARALMALGRPTEANMLLAPLSNEFSFYGQLAADELGTSVS
                     SAPQTYQPSADEVDAIAADRGIQRALALYRAGLRYEGALEWQWSIKDYDDKQLLAAAM
                     LAVRNDWYERAIDTAERTAILHDFGLRYPAPYRKLVQDYSQQLDLDEAWVYGLVRQES
                     RFVHTARSTAGASGLMQLMPATARCVAKRLGMMGHHTTLTETVDSNIGLGTYYLRQVL
                     DSVDKQMVLASAGYNAGPRRARDWAASRPLEGAVYIETIPYPETREYVKKVMNNTLYY
                     ARLFHQSNLSLRDRLGVVPARSMSNN"
     gene            958..1908
                     /locus_tag="VN496_05135"
     CDS             958..1908
                     /locus_tag="VN496_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HXF16364.1"
                     /translation="MKVSSVCVIGGTGFLGSHVVRQLAARGLRVRVPTRRRERAKALI
                     VLPTVDVFNADVHHPATLERAIQDCDAVISLVGILHETRAGDFARIHTELPRKIVDAC
                     RKAGLRRLLHVSALKVAHDAPSAYLRSKAEGEQQIRVAEAGGIRTTIFRPSVIFGPED
                     RFLNMFAQLAQKLPAIVLACPKAIFQPVYVEDVARALLDSLELSQTYGRSFELCGPKT
                     YTLQELVKYVCRLLRLERPILPLNDEMSHLLAWIMEWSPVKLLSRDNYHSMKIANRCD
                     CPFPTVFNFQPSALEAVVPTYLLDITPRARYRWFRFRARH"
     gene            1937..2590
                     /locus_tag="VN496_05140"
     CDS             1937..2590
                     /locus_tag="VN496_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXF16365.1"
                     /translation="MPNLVLVIGNKAYSSWSLRPWLLMKQAGIAFEELRLSLYAKDAR
                     KTLLLHSPTGRVPVLRDAGITIWDSLAISEYLAEKYPRMQLWPADVAARAHARAISAE
                     MHSGFMTLRHQMPMNVRRKLPVRAKSAELDADIARVESIWNECRSRYSAAGPYLFGGF
                     CIADAMYAPVASRLHTYGVPLGGAAGQYAMTIQALPAMQEWIAGARTETEVNPQYEA"
     gene            2587..3813
                     /locus_tag="VN496_05145"
     CDS             2587..3813
                     /locus_tag="VN496_05145"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HXF16366.1"
                     /translation="MRIYAVGGAVRDELLGRPVKDHDYVVIGSSPEEMERLGYRPVGR
                     DFPVFLHPRTHEEYALARTERKTSRGYKGFEVQASPDVTLEQDLARRDLTINAIARDE
                     AGNIIDPYHGVADLTAGILRHVSPAFVEDPVRVLRVARFVARFGFRIAPETLELMREM
                     VHNGEVDHLVPERLWQELSRGLMEEQPSRMLIVLRECGALARVLPEVNALFGVPQPVQ
                     HHPEVDTGLHILLVIDYAAAQHYPLTVRFAALTHDLGKALTPSDVLPKHDGHEHRSVE
                     LLEKLCERLRVPSECRDLALLTARYHGEAHRAQELRPATVLKLLSAADVYRRPERFEE
                     FLAACSCDYHGRSGLEARPYPQAELLRESARAARQVDAGNIARSMDNDSGRIAAAIET
                     ARLDAVSRTLARLRQR"
     gene            3876..4322
                     /locus_tag="VN496_05150"
     CDS             3876..4322
                     /locus_tag="VN496_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HXF16367.1"
                     /translation="MMNRIAVAAVFTCAAFITPNVFAANQSASKLEAAFKKADTDHDG
                     SLTKDEAKTLPRVAKHFDEIDTDKDGKVTLKELQTSMKQVRAEMHERAVQRFKSADKD
                     NDGTLTKEEAKAMPRVAKNFDAIDADKDGTVSEKEIHNYMKTHHPK"
     gene            complement(4374..4571)
                     /locus_tag="VN496_05155"
     CDS             complement(4374..4571)
                     /locus_tag="VN496_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXF16368.1"
                     /translation="MLKWLLTLLIALLVLGALTPWLRRLGFGRMPGDITIERGGKQYS
                     FPVGSTIMLSLLASLIFWMLR"
     gene            4671..5294
                     /locus_tag="VN496_05160"
     CDS             4671..5294
                     /locus_tag="VN496_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HXF16369.1"
                     /translation="MIELYTWSTPNGRKVSIMLEELALPYRVHAIDIGKGQQFGAEFV
                     AVSPNSKIPAIVDSDGPDGTPYSLFESGAILLYLSDKTGRFLPQKKTQRFDAIQWLMF
                     QMGGVGPMFGQVHHFLRAAPEPVPYAIERYGKETRRLYAVLDKHLAGHEWLASEYSVA
                     DIATYPWVARHDWHKVDLADFPNVKRWFEIIAARPAVQQGMKIPTAR"
     gene            5315..6598
                     /locus_tag="VN496_05165"
     CDS             5315..6598
                     /locus_tag="VN496_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXF16370.1"
                     /translation="MDEPLPSKIGKYPVIREIARGGTSRVYLGRDPFANRDVAIKLVS
                     AADGIESDLRRRFHKVFVNEASLVGKLIHPHIMAIYDADASDDFSYIVMEYVDGPTLE
                     HFCNVTNLLPVDRIVEVAFKCSLALDFAHRHGIIHRDIKPANILIGAQGDIKISDFGV
                     ALHTGGDHTHLQGVGSPAYMSPEQVQDKELTHQTDIYSLGVVLYQLLTGKLPFIAANK
                     ASLLYQILNIDPPPPSTYRTDIPEPLDKVVMHALSKDARLRHQTWGEFSRELTQTYRH
                     LTLPPDSISDTEKFNAIRSLGFFKNFRDVEIWETVRIAAFRRIQPEKIIIREGDVGEN
                     FYVVAAGQARVTKANSELDILDPGDCFGEILYFEQTKARRVTTIASITALTVIEIKAL
                     ALQKASDACQKQFNQAFLKILIARLESASDRLSAR"
     gene            6657..7226
                     /locus_tag="VN496_05170"
     CDS             6657..7226
                     /locus_tag="VN496_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HXF16371.1"
                     /translation="MEALLTSILLVAVAEIGDKTQLLSFVLAARLRKPWPIIGGILLA
                     TVLNHTLAGAVGTLLAGWVPADTLSWIVGLSFIGFGLWALHPDALSDSPRLHKAGAFV
                     TAFLAFFLAEMGDKTQFATIALAARFDALMTVVIGTTIGMMLANVPAVWLGKKLADRI
                     PMRAIRLLAATLFILVGAVTLWSTFRTAQ"
     gene            7223..7435
                     /locus_tag="VN496_05175"
     CDS             7223..7435
                     /locus_tag="VN496_05175"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HXF16372.1"
                     /translation="MNSTGPAQKHCPDCGAIFECAMAPDTPCWCSLKFARVMPVPEAG
                     AHCYCPNCLSIRVALEQRKTASKGPV"
     gene            complement(7449..8129)
                     /locus_tag="VN496_05180"
     CDS             complement(7449..8129)
                     /locus_tag="VN496_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXF16373.1"
                     /translation="MDNQFWLGLLEIIGVNIVLSGDNAVVIAMAARSLAPHQRRAAII
                     LGSGAAIVLRVVLTTIAARVLTFPYLKMAGALLLLWIGVQLLVPGKQNNGPAGRSSDH
                     LMAAVRIILVADFVMSLDNVIAVAGAAHGNLVLLILGLALSIPLIIFGSTAILKLMDR
                     FPIVIKLGAGLLGYVAGEMFIGEPVLQAYMQSPIPGMTIPWAGAFLVLGLGAWISNRS
                     NSKTPDPM"
     gene            complement(8210..9094)
                     /gene="sucD"
                     /locus_tag="VN496_05185"
     CDS             complement(8210..9094)
                     /gene="sucD"
                     /locus_tag="VN496_05185"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXF16374.1"
                     /translation="MSILVDKNTKVITQGITGKTGQFHTRMCREYANGKNCFIAGVHP
                     TKAGQDFEGIPIFGSVRAAKQQTGATVSVVYVPPPGAAAAIWEAVEADLDLVICITEG
                     IPVRDMIEVRDRMRKQNRRTLLLGPNCPGAITPEEIKIGIMPGHIHRKGPIGVVSRSG
                     TLTYEAVGQLTEIGLGQSSAVGIGGDPVNGLKHIDILKLYNQDPATEAVVMIGEIGGS
                     DEEEAALWARSNMKKPIVGFIAGVTAPPGKRMGHAGAIISGGKGTAAEKLAVMEECGI
                     KVTKNPAEMGKLLKSVLR"
     gene            complement(9094..10263)
                     /gene="sucC"
                     /locus_tag="VN496_05190"
     CDS             complement(9094..10263)
                     /gene="sucC"
                     /locus_tag="VN496_05190"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXF16375.1"
                     /translation="MKIHEYQAKELVRHYGVTTPRGVACFSVDEAVAAAEKLGGKVWV
                     VKAQIHAGGRGEGGGVKVAKSIEEVRDWASRILGMQLRTHQTGPEGQKVRRLLIEEGA
                     DIRKELYVGLVVDRGRQQVCMMASSEGGMDIEEVAARAPAKIHKAFIDPQLGLADAQV
                     EELSRKIGIPESSLHKARTILKGLYQAFWDTDATLVEINPLILTGDGNVVALDAKMNF
                     DDNALYRHEDIVAMRDLDEEDPAEIEASKHGLSYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGGEPANFLDVGGGATTEKVTEAFKLMLRNPDLRAILVNIFGGIMKCDVIATGVVE
                     AAKQVKLAVPLVVRMKGTNEDLGKKILAASGLPIISAENMAEAGQKVVAAAKGAR"
     gene            complement(10542..10901)
                     /locus_tag="VN496_05195"
     CDS             complement(10542..10901)
                     /locus_tag="VN496_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16376.1"
                     /translation="MPTETFDNMLKTVLMIALMAFAANTMAQSNAPLSLEVAQQRARF
                     ARDQMSDAESKVRVAEKKDQIAKKHLDDAKIQADQAAKALEEAQAAFVAARENHDRAY
                     QDLKRVHDSMRGTPKPQ"
     gene            complement(11021..11096)
                     /locus_tag="VN496_05200"
     tRNA            complement(11021..11096)
                     /locus_tag="VN496_05200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11061..11063),aa:Thr,seq:tgt)
     gene            complement(11135..11557)
                     /locus_tag="VN496_05205"
     CDS             complement(11135..11557)
                     /locus_tag="VN496_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HXF16377.1"
                     /translation="MKETSTTPYLIRAIFEWCCDSGLTPYIAVKVDETTKVPMEYVKN
                     GEIVLNVSPDATRNLKIGNEFLQFSARFAGVSREISVPINSVGGIFAKETGQGLSFQT
                     SPSLPAQVVPSNKSEIVPPNDDHPTPPSGGRPRLQVIK"
     gene            complement(11554..12153)
                     /locus_tag="VN496_05210"
     CDS             complement(11554..12153)
                     /locus_tag="VN496_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXF16378.1"
                     /translation="MMTLYSGTTCPFSQRCRIVLFEKGMDFQIIDVDLFNKPEDLAVM
                     NPYNRTPVLVERDLILYESNIINEYIDDRFPHPQLMPADPVMRARARLFLFRFENELF
                     SNIDAIDSGTQKQAEKARLAVRDNLTQIAPVFVKQKYMLGDEFSMLDVAIAPLLWRLD
                     FYGIQLPKPAAPLMKYAERLFSRPAFIEALTASEKVMRK"
     gene            complement(12174..12899)
                     /locus_tag="VN496_05215"
     CDS             complement(12174..12899)
                     /locus_tag="VN496_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HXF16379.1"
                     /translation="MKKPLLALLLLPVAVYAFGEELRLDSANIDPQDAISIQRGARTF
                     VNYCLNCHSANAMRYNRLMDLGLTEEQIKDNLLFTAEKVGDAMTISANRKDQKQWFGV
                     EPPDLSVIARSRSTDWLYTYLRTFYRDEKRSTGWNNLAFPNVGMPHALWQLQGIQTIK
                     SEIEEHDGHKSEHRTLVLATPGSLSALEYDNVTRDLVNFLSYMAEPERTKRSQIGILV
                     LLFLLLLLAPAWLLKKEYWKDVH"
     gene            complement(12896..>13209)
                     /locus_tag="VN496_05220"
     CDS             complement(12896..>13209)
                     /locus_tag="VN496_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HXF16380.1"
                     /translation="MGLSVMIFFLLPWLDRGAVKSIRYRGAIYKTALAIFVVSFLVLG
                     YLGTEPTNVWGQFGSWLGGAERATVIARIFTIAYFLFFLLMPWYTRIDQTKPEPERVG
                     S"
BASE COUNT         2933 a         3765 c         3593 g         2918 t
ORIGIN      
        1 gcgttattgg cgcgcaagag ccttgatggc gcttggacga ccgaccgagg caaatatgct
       61 gctcgcaccg ctgtcaaacg aattcagttt ctatggccag cttgcggccg atgaacttgg
      121 tacgtcggtg agttccgcgc cacaaaccta ccagccttcg gccgacgagg ttgatgccat
      181 cgcggctgac cgcggaatcc agcgggccct ggcgctgtat cgcgcaggcc tgcgctacga
      241 aggggcgctt gaatggcaat ggtccatcaa agactacgac gataaacaat tgcttgcggc
      301 agcgatgctg gcggtgcgca atgactggta cgaacgcgcg atcgacacgg cggagcgcac
      361 agctatcttg cacgatttcg gcttgcgcta cccagccccc tatcgcaagc tggtacagga
      421 ttactcgcaa caactcgatc tggacgaagc gtgggtatac ggtctggtgc gccaggaaag
      481 ccgctttgtg catacggcgc gttctactgc gggcgcgagc ggcctcatgc agttaatgcc
      541 ggccacagca cgctgtgttg cgaaacgtct tggcatgatg ggtcaccaca cgactttgac
      601 cgagactgtc gatagcaaca tcggtcttgg tacctattat ttgcgccaag tgctcgactc
      661 agtcgacaag cagatggttt tggcatccgc aggatacaac gcgggtccac gccgagcgcg
      721 cgactgggcg gcgagccggc cattggaggg tgctgtttat atcgaaacca ttccgtatcc
      781 cgagacgcgg gaatacgtca agaaagtcat gaacaacacc ctgtactatg cccgcctgtt
      841 ccaccaatcc aatctgagcc tgcgcgaccg tctcggcgtc gtgccggcca ggtcaatgtc
      901 caacaattga atgcgcggat cctataattt cgccaactcc cggttcggat tggaaacatg
      961 aaagtcagtt cggtttgcgt catcggcggc accggctttc tcggcagcca cgtggtgcgc
     1021 cagcttgcag cgcgcggcct tcgcgtgcgc gtgcctacgc gtcggcgcga gcgagcaaaa
     1081 gccttgatcg tgctgccgac agtggatgtc ttcaacgctg atgtgcacca tccagccacg
     1141 ctggagcggg ccatccagga ttgtgacgca gttattagtc tcgtaggaat tttgcacgaa
     1201 acacgcgcag gcgatttcgc gcgcatacat accgaactgc cacgcaaaat cgtcgatgcc
     1261 tgtcgcaaag cgggactgcg ccgattgctg catgtgtccg cacttaaagt cgctcatgat
     1321 gcgcccagcg cctatctgcg ttccaaagca gagggggaac agcagatccg cgtggcagag
     1381 gctggcggca tccgtaccac catttttcgc ccctcggtca tattcggccc tgaagaccgt
     1441 ttcctgaata tgtttgcgca gcttgcacaa aagcttccgg ccattgtatt ggcatgccca
     1501 aaagccatct ttcagccggt ctacgtggag gatgtggcgc gtgctctgct ggatagcttg
     1561 gagctttcgc aaacctatgg tcgcagcttt gagctgtgcg gtcccaaaac gtataccctg
     1621 caagaactag tgaagtacgt ttgccggctg ctgcggctgg agcggccgat cctgccgctc
     1681 aatgacgaga tgtcgcattt gttggcctgg atcatggagt ggtctccggt caaactactg
     1741 tcccgcgaca attatcattc catgaagatt gccaataggt gcgattgccc gttcccgacc
     1801 gtgttcaact ttcagccatc agcgctggaa gcggtggtgc ccacctacct tctcgatatc
     1861 acgccgcgcg cgcgctatcg ctggtttcgg tttcgcgccc ggcactagtt tacattcaga
     1921 cccgggagcc ttcccaatgc ctaatctggt tctcgtaatc ggcaacaagg cctattcctc
     1981 ctggtcgtta cgcccatggt tgctgatgaa acaagccggc atcgcgtttg aagaattgcg
     2041 tttgtcgctg tacgcaaagg atgccaggaa aaccttgctg ctgcattcgc ccacgggcag
     2101 agttccagtg ctcagggacg cggggataac catctgggat tccctggcga tctccgaata
     2161 cttggcggag aaatacccgc gcatgcagct ttggccagct gacgtcgcgg cgcgcgcgca
     2221 cgcgcgtgca atcagcgcgg aaatgcattc aggcttcatg accctgcgcc atcagatgcc
     2281 gatgaacgtc cgccgtaagc tgcccgtccg ggcaaagtca gccgaactcg atgctgacat
     2341 cgcacgcgtc gagagcatct ggaacgaatg ccgcagccga tacagcgctg ccggcccgta
     2401 ccttttcggc ggcttttgca tcgccgatgc aatgtacgcc cccgttgctt cacgtctgca
     2461 tacctacggc gttccgctcg gcggagcggc tggtcaatat gcgatgacga ttcaagcttt
     2521 gccggcaatg caggagtgga tcgctggcgc gcgcacggaa accgaagtca atccgcaata
     2581 cgaagcgtga ggatctatgc cgttggaggg gccgtgcgcg acgagttgct cggccgtcca
     2641 gtcaaggacc acgactatgt cgtgatcggt tcatcgcccg aagaaatgga acggcttggc
     2701 tatcgcccgg tagggcggga tttccccgta ttcctgcatc ctcggacgca cgaggaatat
     2761 gcgcttgccc gcaccgagag aaagacgtcg cgcggctaca agggctttga ggttcaagct
     2821 tcaccagatg tcacgctgga gcaggatctt gcgcgccgcg atctcaccat taatgcgatc
     2881 gcgcgcgatg aggcagggaa catcatcgat ccgtaccacg gggtcgcaga tttgacggca
     2941 ggcatactgc ggcatgtcag cccggcgttt gtggaagacc cggtgcgggt gctgcgggta
     3001 gcgcgctttg tcgcccgctt tggcttccgg atcgcgcccg aaacgctgga gctcatgcgg
     3061 gaaatggtgc acaacggtga agtggaccat ctcgtgcctg aacgcctctg gcaagaattg
     3121 tcacgtggac tgatggaaga acagccctct cggatgctga tcgtgctgcg cgagtgcgga
     3181 gcgcttgccc gggtactgcc ggaagtcaat gcgctgtttg gggtgccgca accggtacaa
     3241 caccatccgg aggtcgatac cggcctgcat attttgctgg taatagatta cgctgcggct
     3301 cagcactatc cgcttaccgt gcgctttgcg gcactgaccc atgatctcgg taaagcgctt
     3361 acgccgtccg acgtcttgcc caagcatgac ggccacgaac acaggagcgt cgaactgctt
     3421 gagaagcttt gcgaacgcct gcgtgttcca agtgaatgcc gggatctggc gttgttgact
     3481 gcacgctatc acggcgaagc gcatcgggcg caggaacttc gtcctgcaac agtactaaag
     3541 ttattgtcgg cggctgatgt gtatcgcagg ccggagcgtt ttgaggaatt cctggctgcc
     3601 tgcagttgcg attatcacgg ccgcagtggc ctcgaagcgc gcccctatcc gcaggcagaa
     3661 ttgttaaggg aatctgctcg ggccgcacgg caagtcgatg ctggcaacat cgcgcggagc
     3721 atggacaatg attccggccg catcgccgcc gcaatcgaga cagcaagact ggatgcagtt
     3781 tccaggacac tggcccgact gcgtcaacgc taaaacagca taatcgttat gattaggacc
     3841 gatgtgctga agaagtccat ctcaaggaga tcccgatgat gaatagaatt gcagttgctg
     3901 ccgtatttac ctgtgccgct ttcataacac ccaatgtgtt cgctgccaac cagtccgcct
     3961 cgaagctgga agcagccttc aaaaaggccg acacggacca cgacggcagc cttaccaagg
     4021 atgaagcgaa aacgctgcca cgggtggcta aacacttcga tgaaatcgac actgacaagg
     4081 acggcaaggt gacactgaag gaactgcaga catcgatgaa gcaggtgcgc gcggaaatgc
     4141 acgagcgcgc ggtacagcgc ttcaagtctg ccgacaagga caacgacggc actctaacaa
     4201 aagaggaggc gaaagctatg ccgcgggttg ccaagaattt cgatgctatc gatgccgaca
     4261 aagacgggac ggtcagcgaa aaggaaatcc acaattacat gaagacgcac cacccgaagt
     4321 aacggcctcg gtaaggcatt acccaatgga caggcacggt gcctgtccat tttttatctg
     4381 agcatccaga aaatcaagct agcgagcagc gacagcatga ttgtggagcc aaccgggaac
     4441 gaatactgtt tgccgccccg ttcgatcgtg atgtcccccg gcatgcggcc aaatccgagc
     4501 cggcggagcc agggagtaag cgcgcccaat accagtagcg caatcaacag tgtcaacaac
     4561 catttcagca cagcggaatt ttaacaaagt caacgatcat taaaccggcg cgcttcgtgc
     4621 gttcgattag aattcgtagt tctcatgtca aatgcttagg ggagttgtca ttgatcgaac
     4681 tgtacacctg gagtacgccg aacggccgca aggtctcgat catgctggaa gaacttgcac
     4741 ttccctacag ggtacatgcg atagacatcg gcaagggaca acaattcggc gcagaatttg
     4801 tcgcggttag cccgaacagc aaaattcccg ctattgttga cagcgacgga ccagatggca
     4861 ccccgtattc attattcgaa tcgggggcga tcctgttgta cctcagtgac aagaccgggc
     4921 gtttcctgcc gcaaaagaag acccagcgct tcgacgcgat tcagtggctg atgtttcaaa
     4981 tgggcggggt cggtccgatg ttcggccaag tgcaccattt cctgcgggcg gcacccgagc
     5041 cggtacccta tgccatcgaa cgctacggta aagagacgcg ccgtctttac gcagtgttgg
     5101 ataagcacct cgccggccac gaatggctgg cttcggaata ttcagtcgca gatatcgcaa
     5161 cttacccctg ggtcgcccgc catgactggc acaaggtgga cttggccgat ttcccgaacg
     5221 tcaaacgctg gtttgaaatc attgccgcac gtccggccgt acagcaggga atgaagatcc
     5281 caactgcgcg ctgaagtcgt tcaaaacctt gaacatggat gaaccgctgc cgtccaagat
     5341 cggcaaatac ccggtgatcc gcgagattgc acgcggcggc accagccggg tgtatctggg
     5401 acgagatcct ttcgccaatc gggacgttgc catcaagctg gtcagcgccg cagatggcat
     5461 cgaatccgat ttgcgtcgtc gttttcacaa ggtattcgtg aatgaggcgt cgttagtcgg
     5521 caaactcatc cacccgcata tcatggccat ctatgacgcc gatgcttcgg acgatttcag
     5581 ctatatcgtc atggaatatg tcgatgggcc gacgctggaa catttctgca acgtgacaaa
     5641 cctgctacca gtagaccgca tcgtggaggt tgccttcaaa tgcagccttg cactggactt
     5701 tgcccatcgc cacggcatta tccaccggga catcaagccg gccaatatcc tgatcggcgc
     5761 gcaaggtgac atcaaaatca gcgattttgg cgtggcgttg cacacgggag gcgaccacac
     5821 ccatctgcaa ggcgtcggat cgccggccta catgtcaccg gagcaggtgc aagacaagga
     5881 actcacgcat cagacggata tctactcgct gggcgtagtg ctgtatcaac tgttgactgg
     5941 aaagttgcct ttcattgcag ccaacaaagc gagcttgctg tatcaaatac tgaacatcga
     6001 tccgccaccg ccatccacgt accgtaccga cattccggaa ccgctcgaca aagtagtgat
     6061 gcacgcgctt agcaaagatg cccgcttgcg tcaccaaaca tggggagaat tcagccgcga
     6121 gttgacccag acttaccgcc atctcacgtt gccgcccgat tcaatatcgg atacggaaaa
     6181 attcaatgcc atccgatcgc tgggattttt caaaaacttt cgtgacgtgg agatctggga
     6241 gacggtacgc attgcggcgt ttcgccgcat ccagccagaa aaaataatca ttcgcgaagg
     6301 tgacgtcgga gagaattttt atgtcgtggc agccggacag gctcgcgtga ccaaagcaaa
     6361 cagcgaactg gacatcctgg acccgggaga ctgtttcggg gaaattcttt atttcgaaca
     6421 aaccaaagcc aggcgcgtca cgaccattgc ttccataacg gcgcttacgg tcatcgaaat
     6481 aaaggcactc gctctgcaaa aggcaagcga cgcgtgccag aagcagttca accaggcctt
     6541 tctgaaaatt ctgatcgcga ggctagagag cgccagcgac cggctgtccg cgcgctagtt
     6601 gagtattcca cgtcgatcat ggtccaagtg tctgtcgacg tttctgagat tctggtttgg
     6661 aagcacttct aacatcgata ctactcgtcg ctgtcgcaga aataggggac aaaacccaac
     6721 tgctctcgtt cgtgctggca gcacggctac gcaaaccatg gcctatcatt ggcggcattc
     6781 tgctcgccac ggtgctgaat catactctgg ccggcgcggt cggaaccctg ctcgccggct
     6841 gggttcccgc ggacactttg tcctggatcg tgggattgtc tttcattggg tttggactct
     6901 gggctttgca tccagacgcg cttagcgatt cgccccggct gcataaggcc ggggcttttg
     6961 tgaccgcttt cctggcgttt ttcttggccg agatgggaga caaaacacag ttcgccacga
     7021 ttgccctcgc agcgcggttc gatgcgctta tgacggtcgt catcggcacc accatcggta
     7081 tgatgctggc caatgttccg gccgtctggc taggtaaaaa attggcagac cgcatcccca
     7141 tgcgcgccat ccgcctgctg gcagcgactt tattcattct ggttggcgca gttactctat
     7201 ggagcacatt ccgtacagca caatgaactc taccggtccc gcacagaaac attgtcctga
     7261 ttgcggtgcg atttttgaat gcgcgatggc tccggacacg ccgtgttggt gcagcctgaa
     7321 atttgcgcgc gtcatgccgg taccggaagc aggcgcccat tgttactgcc caaactgctt
     7381 gagcatacgc gttgcgctgg agcaacggaa aaccgcgagc aaggggcctg tttaagtgcg
     7441 gcgcaaggct acatcgggtc gggtgttttg gaattggatc tattcgaaat ccacgccccg
     7501 agccccaaca cgagaaatgc accggcccat ggaatcgtca tgccggggat gggggattgc
     7561 atatacgctt gcaggaccgg ttctccaata aacatttcgc cggcgacata accgagtaaa
     7621 cccgctccga gcttaatgac aatcggaaag cggtccatca atttcaggat cgccgtgctg
     7681 ccgaagataa tcagcggaat gctgagcgca agtcccagga tcaacaaaac cagattgcca
     7741 tgcgccgcgc cggccactgc aatcacgttg tccaggctca tgacgaagtc tgcgaccagt
     7801 atgattcgta cggccgccat gagatggtcg gatgatcttc cggcaggacc attattttgc
     7861 ttacccggca ccagcagctg aacgccgatc catagcaaga gcaatgctcc agccattttt
     7921 agatatggga atgtgagaac gcgcgccgcg atggtcgtca ggacgacacg caaaacgatt
     7981 gcagctccac tgccgaggat gatcgcggca cgtcgctggt gcggcgccag cgaccgtgca
     8041 gccatcgcaa tgaccaccgc gttgtccccg ctcagcacaa tatttacacc gatgatttcg
     8101 agcaggccga gccagaattg gttgtccatg cccatgcctg tcgtcgtgac ccattggcgc
     8161 aaacgcgatt cttcgcgtca ctgatatgac acgagtgaat cgaatggcgc tagcgtaaga
     8221 cggacttgag cagcttgccc atttccgcgg gatttttcgt cactttgatg ccgcactctt
     8281 ccatgaccgc cagcttttct gcagccgtac ctttgccgcc cgatatgatg gcgccggcat
     8341 gacccatgcg tttgcccggc ggcgcagtga cgccggcaat gaaacccacg atcggttttt
     8401 tcatgttgct tctggcccac aatgccgctt cctcttcgtc ggagccgcca atttctccga
     8461 tcatgactac tgcttcagtc gctggatcct ggttgtacag cttcaggata tcgatatgct
     8521 tcagcccgtt taccggatca ccaccgatcc cgactgccga ggattgtcca agccctatct
     8581 cggtaagctg gccgactgct tcgtaagtca gggtgcctga acgcgatacc acgccgatcg
     8641 ggcccttgcg gtgaatgtgc ccgggcataa tgccgatctt gatttcttca ggcgtgatgg
     8701 ctccggggca attcggtcca agcagtaatg ttctgcgatt ctgcttacgc atgcgatcac
     8761 gcacttcgat catgtcgcgt accggaatac cttccgtgat gcaaatcacc agatccagat
     8821 cggcctcgac ggcctcccag atcgcagcgg cagcccctgg gggtggcacg tacaccaccg
     8881 agacggtcgc gccggtctgt tgcttcgctg ctcgcaccga accaaaaatc ggaatgcctt
     8941 cgaaatcctg ccccgctttg gtcggatgca caccggcaat gaaacaattc tttccgttgg
     9001 cgtattcgcg gcacatccga gtgtggaact gtccggtctt gccggtaatc ccttgcgtga
     9061 tgactttggt atttttatcg acaagaatgg acatcatcgc gctcccttgg cggcagcgac
     9121 gactttctgg cccgcctcag ccatgttctc ggccgaaatg atgggcaggc cggaggcggc
     9181 caatatcttc ttaccaaggt cttcgttggt gcccttcatg cgcacgacaa gcggtacggc
     9241 aagcttgacc tgtttggccg cttcgaccac ccccgtggca attacatcgc atttcatgat
     9301 gccgccaaag atgttcacca aaattgcgcg cagatccggg ttgcgcagca tcagtttgaa
     9361 cgcttcggtg accttctcgg ttgtcgctcc gccacctaca tccaggaaat tcgctggctc
     9421 gccgccgaac aatttgatcg tatccatggt cgccattgca aggccggcac cattaaccag
     9481 gcatccgatg tttccatcaa gagatatata ggacaggcca tgctttgagg cttcaatctc
     9541 cgccggatct tcctcatcga gatcgcgcat cgccacgatg tcttcatgcc ggtagagcgc
     9601 gttgtcgtcg aaattcatct tggcatcgag cgctaccacg ttgccatcgc cagtcagaat
     9661 gaggggattg atttctacca gcgtggcatc ggtgtcccaa aacgcctggt acaggccctt
     9721 gaggatcgtg cgcgctttgt gcaagctgct ttcgggaata ccgatcttgc gggaaagttc
     9781 ctcgacctgc gcgtcggcca gacccaattg gggatcgatg aatgctttgt ggattttcgc
     9841 cggcgcgcgc gcagccactt cttcgatatc catgccacct tcagaggaag ccatcatgca
     9901 gacctgctgt ctgcctcggt ccacgaccag gccgacgtag agctcttttc gtatatcagc
     9961 cccctcttcg atcaacagcc gccgcacctt ctgcccctcg gggcccgttt gatgcgtccg
    10021 gagttgcatg ccaaggattc tcgacgccca atcgcgcacc tcttcgatgg acttggcgac
    10081 tttgactccg cccccctcgc cgcgtccgcc ggcgtgaatt tgtgccttaa ccacccaaac
    10141 cttgccgcca agcttttccg ccgcggcgac cgcttcatcg acactgaaac aagccacccc
    10201 ccgaggcgtg gtcacgccgt agtgccggac aagttccttt gcctgatact cgtgaatttt
    10261 catcgctact taacttcttc gaaattttat tgaatagcgg agcgcgcctg ttcgtgaggc
    10321 gagcaatgaa caaagtttat ttggcaagca gaagaccacc gaaaaaccct gccccaaaag
    10381 cgcgggaagt atactccaag ccccgcctca actgccgcaa tgcagtgtca attcagattg
    10441 ttccgccaaa atcactggtg aagaaacgtg ctttggtatt caactgttca atcgcaacat
    10501 cagcaaagcg cgactaaatt gcaaatcacc aaaagcagtt gtcactgtgg cttgggcgtt
    10561 ccacgcatgg aatcgtgcac gcgcttcaga tcctggtaag cacgatcatg gttttcgcgc
    10621 gctgccacaa atgccgcctg cgcttcctcg agcgctttcg ctgcctggtc tgcctggatt
    10681 ttggcgtcgt cgagatgttt ttttgcaatc tggtcttttt tttcggctac tcgaaccttg
    10741 ctttcggcat cggacatttg gtctcgcgcg aaccgggccc gctgctgagc tacttcaagg
    10801 gaaagcggcg cgtttgattg cgccattgtg tttgcggcaa atgccataag cgcgatcatc
    10861 aaaacagtct tcagcatatt gtcgaaggtc tcagtcggca tttaatcgca atggatatat
    10921 ctgcggcgac atatgaccga gggtggccgg attccaagat gccatattta caatgcttaa
    10981 aacgtctatg aggggttgga catactgggc gactgcttgt tggtgccggc aacaggaatc
    11041 gaacccgtga ccttttgatt acaaatcaac tgctctacca actgagctat gccggcttcg
    11101 ctccaattat agctgccact tctggaacat cagactactt gataacctgg aggcgaggtc
    11161 tcccgccaga cggtggcgtt ggatggtcat cgttgggggg aacaatttcg ctcttgttgg
    11221 aagggactac ctgggccggc agcgacggtg acgtctggaa ggagagaccc tggcccgttt
    11281 ccttggcgaa gatgccgccg accgaattaa tcggcacgga gatttcgcgt gaaacaccgg
    11341 caaacctcgc cgaaaactga agaaattcat taccgatttt taaatttcgg gtcgcatccg
    11401 gactcacatt caggacgatt tccccgttct tgacatattc catcgggacc ttcgtcgtct
    11461 catccacttt tacggcgata tacggcgtaa ggccgctatc gcaacaccac tcgaagatcg
    11521 cacgaataag atacggcgtg gtcgaggtct ccttcacttg cgcatcacct tctcggaagc
    11581 agtgagcgct tcaataaagg ccggccggct gaacaggcgc tcggcgtact tcatcaacgg
    11641 cgccgccggc ttcggcaatt gaatgccata gaaatcaaga cgccacaata atggcgcaat
    11701 cgcgacgtca agcatcgaaa attcatcgcc gagcatgtac ttctgtttca caaataccgg
    11761 tgcaatttgc gttagattat cgcgtacggc gagccgcgcc ttttccgcct gcttttgcgt
    11821 accgctgtct atggcatcga tgttggaaaa cagttcgttt tcgaagcgga agagaaacag
    11881 acgggcgcga gctcgcatta cgggatccgc tggcatgagc tgcggatgcg gaaagcgatc
    11941 atcaatgtac tcattgatga tgttagattc gtaaagaatt aaatcccgtt ctaccagtac
    12001 cggcgtgcga ttgtacggat tcatcaccgc gagatcttcc ggcttattga acagatcgac
    12061 gtcgatgatc tggaaatcca ttcctttttc aaacaggacg atcctgcatc gttggctgaa
    12121 tgggcaagtg gtgcccgagt aaagtgtcat cattggctgt tacctggagt taatcagtgc
    12181 acatctttcc aatactcttt cttgagcaac cacgctggag ccagaagcaa gagtagaaat
    12241 aacagcacca ggatgccgat ttgcgaacgc ttggtccttt ccggttcggc catatacgac
    12301 aggaaattca ccaaatcccg ggtaacgttg tcatattcca gtgccgataa gctgccgggc
    12361 gttgcaagca cgagcgtcct gtgttcggac ttgtgcccgt catgttcctc gatttcgctc
    12421 tttatcgtct ggatgccctg caactgccac aaggcgtgcg gcatgccaac gttcggaaat
    12481 gccagattgt tccagccggt cgatcttttt tcgtcgcgat agaaagttcg caaatacgta
    12541 tacagccagt ccgtgctccg cgaccgcgct atcacggata gatccggcgg ttctacgcca
    12601 aaccattgtt tctgatcctt gcgattggcg ctgatggtca tcgcatcccc gaccttctcc
    12661 gcggtgaata gcaagttatc cttgatctgc tcttctgtga ggcctaagtc catcaagcgg
    12721 ttatagcgca ttgcatttgc cgaatggcag ttcaggcagt aattgacgaa cgtacgggcc
    12781 ccacgttgta ttgaaatggc atcttgcgga tcgatatttg cactgtcgag ccgcagttcc
    12841 tccccgaatg catagactgc aaccggcaaa agcagcagtg cgagtaacgg ttttttcaag
    12901 atcccaccct ttccggctcc ggcttcgtct gatcaatgcg ggtataccag ggcatgagca
    12961 ggaaaaacag gaagtaagcg atcgtaaata tgcgggcgat cacggtggcg cgctcggcac
    13021 cgccaagcca tgaaccaaat tggccccaga cgttggtcgg ttcggtgccg agatagccca
    13081 gaaccaaaaa ggaaacgaca aatatggcca gagccgtctt ataaatggct ccgcgatagc
    13141 gaatcgactt aaccgcgccc ctgtcaagcc agggcaacag aaagaaaatc attaccgaca
    13201 gccccatag
//
LOCUS       DATMZQ010000157         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4898789, whole genome shotgun sequence.
ACCESSION   DATMZQ010000157 DATMZQ010000000
VERSION     DATMZQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4898789"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VN496_05225"
     CDS             <1..1577
                     /locus_tag="VN496_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF16381.1"
                     /translation="RQRTDRVLRESEERFRAALAATSDIVWTNNAQGQMTGEQAGWSA
                     YTGQSREEYQDYGWAEAVHPDDIAVTISKWKEAVASASAFVFEHRVRRRDGTYRNMQV
                     RAVPVTSPDGSVREWVGVHRDITIQRESEAQRAALLERERAARTAAEQASRTKDEFLA
                     NVSHELRTPLNAMLGWSSVLLASPQMGHEDLKKGLATIERNARLQAQIIEDLLDMNRI
                     LSGNLRLDVTPVDVQSVLQAAVETIRPAANAKAIRVETNIAAGSIVPGDSGRLQQVFW
                     NLLSNAVKFTPRGGKVLVGTRTLDSLLEISVQDDGIGMAPDFLPRLFERFCQADASTS
                     RVHGGLGLGLAIVKELVELHGGSVSATSKGEGKGARFSVYLPIMSSQATEEQPALTEL
                     KVSRRVEPSLRGAKVLIVDDERDTRDLLKKILTERGATVVLADSTDEALLQIAKSNPD
                     LLISDIGMPERDGYDLIGQLRNHPAWKAIPAIALTAFAMADDRMQAVRSGYQAHIGKP
                     VMPADLIDTVAALLDR"
     gene            1843..3108
                     /locus_tag="VN496_05230"
     CDS             1843..3108
                     /locus_tag="VN496_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF16382.1"
                     /translation="MPETKKTLKQRRRFIKAAGAVSAAAGIGFPFIARAQSKPIRIGM
                     PTIMSGRVAQLGISSRNSVMTEIEKFNLAGGLGGRKIELITRDSKGKPDEAANNTRQL
                     INGDGCEIIIDAESSAGAFAVQEVIRELKVLCIHTNSETSSLSADPKIRVPTAFRTAR
                     QGIHDAIVSATYAAGIVKANGLKTWMSCSPDYAYGRDNTSLFMQYLKRFQPDIEVVGE
                     AWPKLFQADYTENITKILAAKPQALYSALWAGDLVSFVDQAGIYNLLNQTNMFAINMA
                     DYTTLTAVKNLPQGIHSSNRYIKTFPATADNAAWGDDYKTRYGDYPTNWSWENAVAMH
                     FIVAAMKKTGSTDSTKLAEAIRGMKLKTPFGADGTITMRADDQTTIGYALGWGTTIPK
                     EPYVPHVTPGDWKAILEHEAEWKKTMGYA"
     gene            3189..>3667
                     /locus_tag="VN496_05235"
     CDS             3189..>3667
                     /locus_tag="VN496_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF16383.1"
                     /translation="MDLQAMLACFTSASCLVTQTTGGLISGMLLFLVAAGLSLIFGTL
                     KVINFMHGSLYMLGAYFASTLYNLTGSYAIAVFGAALGVGLFGLLFERLFMSRVYGAD
                     VLMQLLVCYSFILILDDAVKIIWGAEFRSMGMPAAFQRAPVIIAGGVLPVFYLFLIGV
                     "
BASE COUNT          788 a         1086 c         1089 g          704 t
ORIGIN      
        1 cacggcagcg gactgaccgc gtgctgcgtg agagcgagga aaggtttcgc gccgccctgg
       61 ctgccacgag cgatattgtg tggaccaaca atgcgcaggg gcagatgacc ggcgaacagg
      121 ctggctggag tgcctatacc gggcaaagcc gggaggagta tcaggactat ggttgggcgg
      181 aagccgtcca tccggacgac atcgcggtaa cgattagcaa atggaaagaa gccgtggcat
      241 ccgcgtcagc attcgtattc gaacaccggg ttcgccggcg ggacggtacc tatcgcaaca
      301 tgcaggtccg cgccgttccg gtaacaagcc cggacgggtc agtgcgcgaa tgggtcggcg
      361 tccatcgcga catcaccatt cagcgcgagt ccgaagcaca acgtgcggca ctgctggagc
      421 gcgaacgcgc tgcgcgcaca gctgcagagc aggccagccg caccaaagac gaattcctcg
      481 ccaacgtctc gcatgaactg cgtacgccgc tcaacgccat gctcggctgg tccagcgtgc
      541 tgctcgcatc gccgcagatg ggacacgaag atctgaagaa agggctcgct accatcgagc
      601 ggaatgcacg cctgcaagcg cagatcatcg aggacttgct ggacatgaac cggattctgt
      661 ccgggaattt gcgtctcgat gtcactcccg tcgatgtgca atccgtgttg caggcggccg
      721 tggaaacaat ccggccggct gcgaatgcaa aagcaatccg ggttgaaacc aatatcgcag
      781 ctggctccat tgtgccgggc gattcgggac gcctgcagca agtgttctgg aacctgcttt
      841 ccaatgccgt caagttcaca ccgcgcggcg gaaaagtcct ggttggcacg cgtacgctgg
      901 attcgttgct cgaaatttct gtccaggatg atggcatcgg tatggcgcca gatttcctgc
      961 cccggctgtt cgaacggttc tgccaggcgg acgccagcac ctcgcgagtg cacggtggcc
     1021 tgggcctcgg tctggcaatc gtcaaggaac ttgtggaatt gcacggtggt tcggtttccg
     1081 caaccagcaa gggcgaaggc aaaggagccc gcttcagcgt ctatctgccg atcatgtcgt
     1141 cgcaggcaac ggaagagcag ccggccctca cagaactaaa ggtttcccga cgagtggaac
     1201 cgtctctgcg cggcgccaag gtgttgattg tggatgatga gcgagatacg cgtgacttgc
     1261 tgaagaaaat cctgacggaa cgcggcgcca cggtggtcct cgcggattcc accgacgagg
     1321 cgctgttgca aattgcgaaa tccaatccgg atctgctgat cagcgatatc ggcatgccgg
     1381 aacgcgatgg ttacgatctc atcggacagc tcagaaatca tcccgcctgg aaggccattc
     1441 ctgccatagc cctgaccgca ttcgccatgg cagatgatcg catgcaggca gtccgctccg
     1501 ggtatcaggc tcatatcggc aagcccgtga tgccggcgga ccttatcgac actgtcgcgg
     1561 cactgctgga ccgctgaatt agatcagggc cggcctggcc gattgtcaaa ccgcccgcgg
     1621 gcgcgaatcg gggcatgcat cacgcaagcc agtggctcgt tgtagagcgg gcagcataat
     1681 gcgaccggca ttgttctcca tgctttcacg tcacgcattc cgcatgctac tgtagttcct
     1741 cgcgcaggat ttcgaacccc catcgcggaa gctgccgtgt gggggataca atcaaaaaag
     1801 tttgcgagta tcgcaggacc acaaaatcga gaggagacgc ccatgcctga aacaaaaaaa
     1861 acgctgaagc agcgccgccg gttcatcaaa gccgcaggcg cggtgagcgc ggctgccggg
     1921 ataggattcc cgtttattgc gcgcgcacaa agcaaaccca tccggatcgg catgccgacc
     1981 atcatgtccg ggcgtgtcgc gcagctcggt atctcctcgc gcaactccgt catgaccgag
     2041 attgaaaaat tcaacctcgc cgggggcttg ggtggacgca agattgaact gattacacgc
     2101 gattcgaagg gcaagccgga cgaagcagcc aataacacgc gccagctgat taacggtgat
     2161 gggtgcgaga tcatcatcga tgccgaatcc tccgctggcg cttttgcagt gcaggaagtc
     2221 attcgtgaac tgaaggtgct gtgcattcac accaattccg agacttcctc gctatcggct
     2281 gatccgaaga tccgcgtccc aaccgctttc cgtaccgcgc gccaggggat tcacgacgcc
     2341 atcgtcagcg cgacttacgc cgcgggcatc gtcaaggcca acggcctgaa gacctggatg
     2401 agctgctctc cggattatgc ttacggccgt gacaacactt ccttgttcat gcaatacctg
     2461 aagcgttttc agccggatat cgaagtcgtc ggcgaagcgt ggccgaaact attccaggcc
     2521 gactacaccg agaacatcac caagatactg gcggcgaagc cccaagcctt gtactcggcg
     2581 ctgtgggcgg gcgatctggt ctcgttcgtg gatcaggcgg gcatttacaa tctgctaaat
     2641 cagaccaaca tgttcgccat caacatggcc gattacacaa cgctcaccgc ggtgaagaat
     2701 cttccgcagg gtatccattc ctccaaccgc tacatcaaga ctttcccggc gactgcggac
     2761 aatgccgcct ggggtgacga ctacaagacc cggtacggag actacccgac caactggtcc
     2821 tgggaaaacg ccgtggcgat gcactttatt gtcgccgcga tgaaaaaaac cggttccacc
     2881 gacagcacga agctggccga agcgattcgc ggcatgaagc tcaagacgcc gtttggcgcg
     2941 gacgggacca tcacgatgcg cgccgacgat cagacgacga tcggttacgc gctgggttgg
     3001 ggcacgacga tcccgaagga accgtacgtg ccgcacgtca caccgggcga ctggaaagcc
     3061 atcctggaac acgaggcgga gtggaagaaa acgatgggtt acgcctgacg cgcgacccgt
     3121 ccgaaccaat gcgcggcagc caattcgcgc tgccatgatt acgctgtcct tccgacccgg
     3181 agtagacctt ggatctacaa gccatgctgg cgtgctttac gtccgcgtcc tgtctggtca
     3241 cgcagaccac gggcggactc atcagcggaa tgctgctgtt tctggtcgca gccgggctgt
     3301 cactgatatt cggcacgctc aaggtcatca acttcatgca tggctccctt tacatgctcg
     3361 gagcctactt cgcatctacg ctctacaacc tcaccggcag ctacgcaatc gcggtattcg
     3421 gcgccgcact cggtgtgggc ctgttcggcc tgctgttcga gcgtttgttc atgagccgcg
     3481 tgtacggcgc cgacgtgctc atgcaattgc tggtgtgcta cagcttcatc ctgatcctcg
     3541 atgacgctgt caaaataatc tggggggctg aattccgctc gatgggcatg ccggcagcat
     3601 tccagcgagc gccggtgatc atagcgggtg gcgtgttgcc ggtgttttac ctgttcctga
     3661 tcggcgt
//
LOCUS       DATMZQ010000158         5216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2543436, whole genome shotgun sequence.
ACCESSION   DATMZQ010000158 DATMZQ010000000
VERSION     DATMZQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543436"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..823)
                     /locus_tag="VN496_05240"
     CDS             complement(2..823)
                     /locus_tag="VN496_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXF16384.1"
                     /translation="MSRSGKALATAPAVRLYRAFRLLLHVLAGLLEIALLFPFYSRLR
                     RNRAVGRWSARLPAILGVRIRMHGHPPRELHRAAVVVANHVSWLDIQIIHSVWQVRFV
                     AKSEVRQWPVIGWMSARAGTLFIERGKGRHAARINQTIHAAISRGDAIGIFPEGSTTD
                     GTELTRFHASLLQPAVVEGAVVHPIALRYRDADGNINVDASYVGETTLAESLHKILAQ
                     RTILAEMIFLPPIDASNKSRRELAAQTQSAIAAALNLPISDRKPDMSAGPRGVPR"
     gene            complement(850..1785)
                     /locus_tag="VN496_05245"
     CDS             complement(850..1785)
                     /locus_tag="VN496_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXF16385.1"
                     /translation="MRTLICGSIAYDTIMVFKDRFKNHILPDQLHVLNVSFLVPDMRR
                     EFGGCAGNIAYSLKMLGGDPLIMATVGQDYQPYAYRLEKLRLAQTHVRQVADTFTAQA
                     FITTDQDDNQITAFHPGAMNFSHHNHVSDARDVGLGIIAPDGREGMLQHAREFHEAGI
                     PFIFDPGQGLPMYSGEELMGFISQADYVAANDYEARLLQDRTGKTIEDLSKLVKALIV
                     TRGAQGSQVFVNAKAQTIPPVPTTQVVDPTGCGDAYRAGLLYGIASDFDWATTGRLAS
                     LLGSLKIAHRGGQNHQFTRDEISERFREAFHRAPW"
     gene            complement(1820..2818)
                     /locus_tag="VN496_05250"
     CDS             complement(1820..2818)
                     /locus_tag="VN496_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXF16386.1"
                     /translation="MTIGALVRAHRRAILVTRISAIWLAALSAIMLAGCGSSPSRPSG
                     ATKPGGYYLDDGPGSNPPANLDAISDAVPKLEPINRSTSRPYTALGHAYRPYTELTPY
                     KARGVASWYGKRYHGQKTSSGEIYDMYAMTAAHTVLPLPSYARVTNVANGKSVVVRIN
                     DRGPFHQDRLIDLSYLAAQRLDLLGTGSGMVEVEAIIPGLEPRPPVIVAAEPAGPERV
                     GRSERNSSPESAVAAEPASTSERIPAPELGTPNAETSAGGFYVQLGAFSVAQNADDFL
                     RKMRMDLDWLADSIRMDRVGALYRVHAGPYAVRTEADRAADRVRKELGFKALVLER"
     gene            complement(2888..3982)
                     /gene="rodA"
                     /locus_tag="VN496_05255"
     CDS             complement(2888..3982)
                     /gene="rodA"
                     /locus_tag="VN496_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HXF16387.1"
                     /translation="MINRLLAYLVKHIDRYLMGLVTALLLMGLLTLFSASGQSSERVI
                     GQAVNICAALAVMWIAANVPLHFLSRIALPLYLVGLTLLVGVAVAGEVVNGARRWLNL
                     GFTRIQPSELMKIAVPLLLAWYFDRYEAILKMRDFAVGAVLVVVPVLLIARQPDLGTA
                     LLVTAAGSYVLFLAGLSWKILLGLGVLGSASVPFLWSMLHDYQRQRVMTLLDPSQDPL
                     GAGYHTIQSTIALGSGGFLGKGWLNGTQSHLDFLPERTTDFIFAVYAEEFGMIGNLVL
                     MVLFLLIIGRGLVIAANAPTLFTRLIAGAVTLTFFTYAFVNMGMVSGILPVVGVPLPL
                     ISYGGTSLVTILFGIGLLMSVHTHRRLVQT"
     gene            complement(3975..>5216)
                     /gene="mrdA"
                     /locus_tag="VN496_05260"
     CDS             complement(3975..>5216)
                     /gene="mrdA"
                     /locus_tag="VN496_05260"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXF16388.1"
                     /translation="VTGYEHVETDANGKSVRTLRSILPVSGNNLTLALDARLQQIAEQ
                     AFGEHRGSLVAIEPSTGGVLALVSKPGFDPNLFVEGIDPQNWDALNNSPDHPLNNRAL
                     QGMYPPGSTFKPFMALAGLELGKRKATFTISDPGYFTLSGGGGHLYRDWKAGGHGIVD
                     LHKAIVQSCDTYFYGLAQDLGIDAIHQFIGRFGLGQKTGIDLDGEVAGLLPSQEWKQR
                     RFQQKWFVGDTISVGIGQGYNLATPLQLAFATAILANDGRVFRPHIVKQIQDSQTDAV
                     TVIEPQPRAELPLSTENIKRVRDAMIDVTKPGGTAAGAGMNAKYLFAGKTGTAQVIGM
                     KGQKYEENKVDERHRDHALFIAYAPADDPKIALAILVENGGHGSSTAAPIARQVLDFY
                     LLGKEPPPPKPIVQEEEQEND"
BASE COUNT         1113 a         1526 c         1499 g         1078 t
ORIGIN      
        1 ctcagcgcgg tacgcctcga ggaccggcgg acatatcagg tttcctgtcc gaaattggca
       61 gattgagtgc agcggcgatt gcgctttgcg tttgcgctgc cagttcgcgt cgggatttgt
      121 tgctggcatc gatggggggc agaaaaatca tttcggccaa aatggtccgt tgcgcaagaa
      181 ttttatgcag tgattcggcc agggttgtct cgcccacata gctggcatcc acattaatgt
      241 tgccgtctgc atcgcgatat cgcaacgcta tcggatgcac cactgcaccc tccaccacgg
      301 caggctgaag caatgaggcg tgaaaacgcg tgagttcagt gccatccgtg gtcgagcctt
      361 caggaaaaat accaatggca tcaccgcggc tgattgctgc gtgtatggtt tgattgatgc
      421 gggcggcatg gcggcctttg ccacgctcaa tgaacaaggt tccggcgcgt gcagacatcc
      481 agccgatcac cggccattgg cggacctcgg atttggctac gaatctgacc tgccataccg
      541 aatgaatgat ttgaatatcc agccacgaca catgattcgc gaccaccact gcggcgcggt
      601 gcaactcgcg aggaggatgt ccatgcatcc tgatacgcac acccagaatg gcaggcaacc
      661 gcgccgacca gcgtcccacc gcgcgattgc gccgtagcct gctgtagaac ggaaacagca
      721 atgcgatctc gagtagtccg gccaagacat gcagcagcag gcggaaagcg cgataaagcc
      781 ggaccgccgg cgcagtcgcc agggcctttc cgctgcggct caccggtaac ttttcgcgat
      841 attcacggct taccagggtg cgcgatgaaa cgcctcgcga aaacgctcac tgatttcgtc
      901 acgcgtgaac tgatgatttt gcccgccgcg gtgcgcgatc ttcagtgatc cgagcagcga
      961 cgcgagcctg ccggtggttg cccagtcgaa atcgcttgcg atcccgtaca gcagtcctgc
     1021 ccgataggcg tctccgcaac cagtcggatc caccacctgc gtagttggca ccgggggaat
     1081 ggtttgcgct tttgcgttga caaaaacttg cgagccttgc gcgccgcgcg tgacgatcag
     1141 tgccttgacc agtttggaca ggtcttcgat ggtcttgccc gttcgatcct gcaacagcct
     1201 tgcctcataa tcgttggccg cgacatagtc ggcctggctg atgaaaccca tcagttcttc
     1261 cccgctgtac attggcaatc cctggcccgg atcgaagata aacgggatac ctgcctcgtg
     1321 aaattcacgc gcatgctgca gcatgccttc acggccgtcg ggcgcgataa ttcccagccc
     1381 gacatcgcgc gcatctgaaa cgtgattgtg gtgtgaaaag ttcatcgccc cgggatggaa
     1441 tgcggtgatt tgattgtcat cctggtctgt cgtaatgaag gcctgggccg tgaaagtatc
     1501 cgcgacctgc cgcacgtgcg tctgtgccag ccgcagtttc tccaaccggt acgcatacgg
     1561 ctggtagtcc tgcccgacgg ttgccatgat cagcgggtca ccgccgagca tcttgagcga
     1621 ataggcaatg ttgcccgcgc atcccccgaa ttcccggcgc atgtccggca ccaggaagga
     1681 aacattcaac acatgaagct ggtcgggcag aatatgattt ttgaagcgat ccttgaacac
     1741 catgatggtg tcgtaggcga tggaaccgca gatcagcgtg cgcatggtgt agtccaggga
     1801 ttatcaaatt ggttgtgcat cagcgctcaa gcaccagtgc tttgaagccg agctccttcc
     1861 gtacgcggtc agcggcgcgg tcggcctcgg tacgaacagc atatggaccg gcgtgcaccc
     1921 ggtacagcgc tcctacacgg tccatccgta ttgaatcagc cagccagtcc agatccatgc
     1981 gcattttgcg caggaagtca tcggcgttct gggcgacgga gaacgccccg agttgcacat
     2041 agaatccacc cgcgctcgtt tcagcgttag gcgttccgag ctccggcgcg ggtatgcgtt
     2101 ctgatgttga ggcaggttcg gcggcgaccg ctgattccgg actggaattg cgttccgatc
     2161 tgccgacacg ttcaggaccg gcaggttcgg cagctacaat cactggtggc cgcggttcga
     2221 gcccgggaat gatcgcttcc acctcaacca tgccgctgcc cgttccgagc aaatccagcc
     2281 gttgagccgc caggtaagac agatcaatga gtctgtcctg gtgaaacggt ccgcgatcat
     2341 tgatgcgcac aaccaccgac ttgccatttg cgacgttcgt tacacgcgca tagcttggca
     2401 gcggcagaac ggtgtgggcc gctgtcatcg catacatgtc atagatctcg cccgacgagg
     2461 ttttttgtcc gtgataacgc ttgccgtacc aactcgccac gccgcgcgcc ttgtaaggcg
     2521 tcagctctgt ataaggtctg taggcgtggc ccagcgcggt atagggtcgg ctggtggaac
     2581 ggttgatcgg ttcgagcttt ggcaccgcat cggagatggc atccaggttt gcgggcggat
     2641 tggaccctgg cccgtcgtcc aggtaatacc ctcctggctt ggtggcaccc gacgggcggg
     2701 acggactgct accacaaccg gccagcatga tggcgctcaa cgcggctaac caaatcgcgg
     2761 agatccgggt cacgaggatg gcgcgacggt gtgctcgcac aagcgcgcca atagtcatcg
     2821 cggcctgcca gaacagcttg caagcgcatt gcaaacgaca tattcgcgcg gcgagcagtt
     2881 caccgtctca ggtttgcaca agtcttcggt gggtatgaac actcatcagc aatccgatgc
     2941 cgaaaagtat ggtgaccagc gaagtgccgc catagctgat cagtggcaaa ggcacgccga
     3001 ccaccggcag gatgccgctg accatgccca tgttgacaaa cgcataggta aaaaatgtca
     3061 acgtaaccgc gcctgcgatc aagcgcgtga acagggtcgg ggcattggcc gcgatcacca
     3121 ggccacggcc gatgattagc aggaaaagta ccatcaatac cagattgccg atcatgccaa
     3181 actcttctgc gtaaacggcg aagataaaat cggtggtccg ttccggcagg aaatcgagat
     3241 gagactgtgt gccgttcaac cagcccttgc ccagaaagcc gcccgatccc agcgcgatgg
     3301 ttgactggat cgtgtgatag cccgcgccaa gtggatcctg tgagggatcg agcaacgtca
     3361 tgactcgctg gcgctggtag tcatgcagca ttgaccataa gaatggcacg ctcgcgcttc
     3421 ccaacacgcc caatcccagc aaaattttcc aggacagacc cgcgaggaat agcacatacg
     3481 atcctgccgc agtaaccagc agcgcggtac cgagatcggg ttggcgcgca atcaacaaca
     3541 ccggtacgac gaccaacacc gcgcccacgg cgaagtcccg catcttcaaa atggcttcgt
     3601 agcgatcgaa ataccatgca agcagcagcg gtacggcgat cttcatgagt tccgacggtt
     3661 gaatgcgggt gaagccgaga ttaagccagc gtcgggctcc gttgacaact tcgcccgcaa
     3721 cggcgacgcc gacaagcaag gtcaacccga ctaaatacag cggtaatgcg atgcgtgaaa
     3781 gaaagtgcag cggcacgttc gccgcaatcc acataacggc cagggcagcg caaatattga
     3841 cggcttggcc gatcacccgc tcggaacttt gtcccgatgc gctaaataaa gtgagcagac
     3901 ccatgagcaa cagtgccgtt accagcccca tgagatatcg atcgatgtgc ttcaccaggt
     3961 aagccagcaa ccggttaatc attctcctgt tcctcttcct gaacgattgg cttgggaggt
     4021 ggcggttcct ttccgagcag gtaaaaatcg agcacctgac gagcgatcgg tgcagcggtg
     4081 ctgcttccgt gtccgccgtt ttcaaccagg atcgccagcg caatttttgg atcgtcggcc
     4141 ggcgcatagg caatgaataa tgcatgatcg cggtggcgtt cgtcgacctt gttctcctcg
     4201 tatttctggc ctttcatgcc gatcacctgc gccgtgccag tcttgccggc aaacagatat
     4261 tttgcgttca tgccggcccc ggccgcggta ccgccgggct tggtgacgtc gatcattgca
     4321 tctcgcacgc gtttgatatt ttccgtcgac agcggcagtt cggctcttgg ttgaggttct
     4381 atgaccgtta cggcatcagt ctggctatcc tggatttgct tcacgatgtg cggccggaac
     4441 acgcgcccgt cattggccag tatcgccgta gcgaaagcaa gctgcagcgg cgtagcaagg
     4501 ttgtaccctt gtccgatccc aactgaaatc gtgtcgccga caaaccattt ttgctgaaag
     4561 cgtcgttgct tccattcctg ggacggcagc aagccggcaa cttcaccatc caggtcgata
     4621 ccggtcttct gtcccagacc aaagcggcca atgaactggt ggatggcatc gataccgagg
     4681 tcctgtgcga ggccgtaaaa ataggtatcg catgactgca cgatagcctt gtgcagatcc
     4741 actatgccgt ggccaccggc cttccaatcg cgataaagat gaccacctcc gccgctcagc
     4801 gtgaaatagc ccggatcgct gatggtgaaa gttgctttgc gcttgcccag ttcgaggccg
     4861 gccagcgcca tgaacggttt gaacgtggat cccggcggat acatgccctg cagtgcgcgg
     4921 ttgttcagcg gatggtctgg tgaattattc agcgcgtccc agttctgtgg atcgatgcct
     4981 tccacgaaca gatttggatc aaagcccggt ttggatacca gagccagcac gccgccggtg
     5041 gagggctcga tggccaccag cgagccgcga tgctcgccaa acgcctgctc ggcgatttgc
     5101 tgcaatctgg catccagtgc caacgtaaga ttgttgccgg aaaccggaag aatgctgcgc
     5161 agcgtccgca ccgacttgcc attcgcgtcc gtttcaacat gttcgtagcc ggtgac
//
LOCUS       DATMZQ010000159         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3236790, whole genome shotgun sequence.
ACCESSION   DATMZQ010000159 DATMZQ010000000
VERSION     DATMZQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236790"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VN496_05265"
     CDS             <1..183
                     /locus_tag="VN496_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16389.1"
                     /translation="KWLMRKFGLDMGPFPPVPWLLATLFVVVPLALLSATWLSFAILA
                     IFIGAVTPITYAKLER"
     gene            303..896
                     /locus_tag="VN496_05270"
     CDS             303..896
                     /locus_tag="VN496_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16390.1"
                     /translation="MSTTIRALRLLPALVVLLPNVLLAASSEIAIQNTAIEKMLKQEL
                     FIDRGRYNLVQETRCQYAYLDSPAVSISDGRVRIKVRLSGKLGMEVGEKCVGAGSDVV
                     RLTVSGKPTYAAEKIGLTDIRLDEVSNEAYRFLLQQFLESAVPRAVSVDVREGLQRVL
                     ADRQAKVEVTVSQLTVTELTAEDNLLHAMLTFSLVAR"
     gene            1010..2104
                     /gene="dctP"
                     /locus_tag="VN496_05275"
     CDS             1010..2104
                     /gene="dctP"
                     /locus_tag="VN496_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXF16391.1"
                     /translation="MQRRSFLGKAVAGVAAGAIAAPAIAQSQPAIQWRMAASWPKSLD
                     TIYGGAELIAKRVAELTDGKFQIRPFAAGEIVPAAQVLDAVQAGTVELGHTALYYYFG
                     KDPAFALACSVCFGPNTRQAIAWWLHGGGEQAAEPLLQEYGLKAILAGSTGCQMGGWF
                     RKEIKSVEDFKGLKFRIGGMAGLVLAKLGAVPQMIGGPDIYPALEKGTIDGAEWVGPY
                     DDEKLGFNKVAKYYYYPGFWEGGPLLHTLVNANKWAELPKSYQAALATACGEGIAWMP
                     AKYDAQNPAALRRLIAGGTKLRPFPKSVLEASEKAAYELYDELGAKSAHWKRMYPAWK
                     KFRDEQFLWFRVAESTYDNYAFYSKVRERK"
     gene            complement(2226..>2414)
                     /locus_tag="VN496_05280"
     CDS             complement(2226..>2414)
                     /locus_tag="VN496_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16392.1"
                     /translation="SPFGSGVASITVDSSGKFVYALVENAIAGFAIDPSTGALTQIAG
                     SPLASPTGSSITTTGAIQ"
BASE COUNT          515 a          671 c          754 g          474 t
ORIGIN      
        1 aaatggctga tgcgcaagtt cggcctggac atggggccgt ttccgccggt gccttggctg
       61 ctggcaactt tgtttgtcgt ggtgccgctg gcattgctct ccgcgacctg gttgagtttc
      121 gccatcctgg cgatctttat cggtgccgta acgccgatca cgtacgcgaa gctggaacgc
      181 tgagggcaac gccgcgtcgg ccggtcgcgg cgcgttttta cgcttccatc cggctccggg
      241 gcacgatacg ctggccctgt ctgagttcaa acaagccctg cacagggcat tgaggcaaat
      301 acatgagtac gactatcaga gcgctgcgct tgctgccagc gctcgttgtg ctattaccca
      361 acgtgttgct ggctgcttcc agcgagattg ccatccagaa caccgcgatt gaaaagatgc
      421 tgaagcagga attgttcatc gatcgcggcc gctacaacct ggtgcaggaa acgcgctgtc
      481 agtatgcgta cctcgattca cccgcggttt cgatatcgga cggcagagtt cgcatcaagg
      541 tccggttgag cggcaagctt ggaatggagg tcggtgaaaa gtgcgtcggc gcaggaagcg
      601 atgtggtacg gctcacggta tcaggcaagc ctacttatgc cgcagaaaag attggcctga
      661 ccgatatccg gctcgacgaa gtttcgaacg aagcttaccg gtttttgctg caacaattcc
      721 tggaaagtgc cgtgccgcgc gcagtaagcg ttgatgtgcg cgaaggcttg cagcgcgtcc
      781 tcgccgaccg ccaggcgaaa gttgaggtta cggtgagcca gcttacggtg actgaactga
      841 cggccgaaga caacctgctt cacgcgatgc tgacgttttc tctcgtggct cgataacagc
      901 agagcgcaca gcccgccagc tgaaccacta tttctttgtc attgcggtgg ccttgagcgc
      961 gtgctgccac tagaatcgag gttcactcaa ttcacgggcg gaggcactca tgcagcgcag
     1021 atcgtttctc ggcaaggccg tagcaggcgt agcggcgggt gcgatcgcag cgccggcaat
     1081 tgcccagtcg cagccggcta tccagtggcg catggcggca agctggccga agagcctcga
     1141 caccatttac ggcggagccg aactgatcgc aaagcgcgtc gccgaactca ccgacggcaa
     1201 attccagatt cgccccttcg ctgcgggaga gatcgtgcca gccgcacagg tgctcgatgc
     1261 cgtgcaggcg gggacagtgg aactcggaca caccgcgctg tattactact tcggcaagga
     1321 tcccgcgttc gcgctcgctt gctcggtatg ctttggaccc aatacccgcc aggcgattgc
     1381 gtggtggttg catggaggcg gcgaacaggc cgctgagcct ttgctgcagg agtacggact
     1441 caaggcaatc cttgccggca gtaccggctg ccagatgggc ggctggttcc gcaaggaaat
     1501 taaatcggtc gaggatttca aggggctgaa attccgcatt ggcggtatgg ccggactggt
     1561 gctggcgaag ctcggcgccg tgcctcagat gatcggcgga ccggatatct atccggcgct
     1621 ggagaaagga accatcgacg gggcagagtg ggtggggccg tacgatgacg aaaaattagg
     1681 gttcaacaag gtggccaagt actactacta tcccggcttc tgggaaggcg gcccgctgct
     1741 gcataccctg gtcaacgcca acaaatgggc cgagctgccg aagtcttacc aggccgcact
     1801 ggccacggcg tgcggggaag gcattgcgtg gatgccggcc aaatatgatg cgcagaatcc
     1861 agccgcgctg cgccgtctca tcgcaggcgg caccaagctg cgcccgtttc cgaaatccgt
     1921 gctggaagcc tccgagaaag ccgcctatga actgtacgat gaactcggcg caaagagcgc
     1981 gcattggaaa cgcatgtatc cggcctggaa aaaattccgt gacgaacagt tcctgtggtt
     2041 ccgcgtggcc gaatcgactt acgataacta tgcgttctat tcgaaagtcc gcgaacgcaa
     2101 gtaggtaccc cgcggccggc catacgatgc aaggatacgt ggacaggcta cgcgcaaagt
     2161 cgttgaccac ataaacgaac ttgccgtggg gaaagtcaag gcgggttaat taagttgatg
     2221 aggcgttact ggatcgcgcc ggtcgtggtg atagatgaac cagtcggcga cgcaagtgga
     2281 ctgccagcaa tctgcgtcag cgctccggtc gaaggatcaa tggcgaaacc agcgatggca
     2341 ttttccacca gcgcgtaaac gaacttgcca gaggagtcga cggtaatgga ggcgacaccg
     2401 cttccaaatg gact
//
LOCUS       DATMZQ010000160         4719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2137795, whole genome shotgun sequence.
ACCESSION   DATMZQ010000160 DATMZQ010000000
VERSION     DATMZQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137795"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VN496_05285"
     CDS             <1..535
                     /locus_tag="VN496_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXF16393.1"
                     /translation="KADAGNYPTLDAVGSLTDNYASNSVIGIRSDLRSLIVGVQLNIP
                     LYQGGGISSRVREAVAGQERARQDLENARRTVAQQTREAFLGVTSGLGQIKALRQAVG
                     STTLQLESTKLGQEVGVRTAVDVLNAEQQLAAAQRDLAQAVYNTILSQLKLKAAVGKL
                     AEADLADINGLLKEDAK"
     gene            549..1139
                     /locus_tag="VN496_05290"
     CDS             549..1139
                     /locus_tag="VN496_05290"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HXF16394.1"
                     /translation="MTTGRAEASEDIVQAKAVLRSQILARRDATDVETRGQYSSTITA
                     KLLALPAYRAAGVIAAYASFGSEFDTSRFLAAALAEGKQLVLPRINRALRALELRQVI
                     DLNGDLMAGVWGIPEPAPRCTMVDPKKMDFMLVPGVAFSPSGARLGYGGGFYDRLLAS
                     LDSQTVRIAAAFQLQMVAQLPEAPHDQRVHAVITEQ"
     gene            complement(1151..2398)
                     /gene="waaA"
                     /locus_tag="VN496_05295"
     CDS             complement(1151..2398)
                     /gene="waaA"
                     /locus_tag="VN496_05295"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HXF16395.1"
                     /translation="MLTRALYTLALYLLLPWALLHLLLRARRQPAYLHHVGERFGFFP
                     DRVSPSVIWIHAVSVGETRAAEPLVKSLQTHYPQHRILLTHGTPTGRQTSLELYGERV
                     DRCYLPYDFTWASRRFLQHFRPIAGIFMETEIWPNLVHASAESAIPLYLVNARMSAKS
                     ARGYRRMGALTGNTLSRLSHIGAQTEQDAQRLRELGARNVTITGNIKFDRIAPTEMVN
                     LGVRLRNAFGAQRPVFLAASTREGEEALILDTLSKIDMPRLLTVVVPRHPQRFEDVGK
                     LITQRGIKVQRRSENAPIAGDTLVVIGDSMGEMFAYYAACDVAFVGGSLLPLGGQNLL
                     EACAVGCPIIVGPHTFNFEEATRGAIEAGAAIRVTDAQDLAKAIERLLHDANLREAMS
                     TAGKNFTNAHRGATAKTLELLEW"
     gene            2563..3636
                     /gene="rfbB"
                     /locus_tag="VN496_05300"
     CDS             2563..3636
                     /gene="rfbB"
                     /locus_tag="VN496_05300"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HXF16396.1"
                     /translation="MTQKSIIVTGGAGFIGANFVQQWIATETAALLNLDKLTYAGNLA
                     SLQSVQSNPKYDFIRGDIGDFDFVSATLADRKPMAVVNFAAESHVDRSIHGPAQFVQT
                     NLVGTFQLLEAVRGYWSALDSDSRKRFRLLHVSTDEVYGSLHEHDAPFTERTPYAPNS
                     PYSASKAGSDHLVRAYHHTYGLPTITTNCSNNYGPYQFPEKLIPLIILNALAGKPLPI
                     YGDGLNVRDWLYVGDHCTAIRLALAKGRPGETYNIGGNSERTNIDVVRTVCRMLDELR
                     PAPSGKRYESLIAYVADRPGHDRRYAIDATKSSAELRWEPTETFESGMRKTVRWYLDN
                     PHWVNGVVSGDYRKWMEKNYSER"
     gene            3657..4016
                     /locus_tag="VN496_05305"
     CDS             3657..4016
                     /locus_tag="VN496_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXF16397.1"
                     /translation="MKNEIRPHYRLEAWKESITLVKAIYEATRTFPKEETYGLASQMR
                     RAAISVPSNIAEGAARTGSREFAQFLNVSRGSLSELETQLLIAVELRYIAADHNVFAR
                     LDKVSRLLTGLHKSVKE"
     gene            4072..>4719
                     /gene="rfbA"
                     /locus_tag="VN496_05310"
     CDS             4072..>4719
                     /gene="rfbA"
                     /locus_tag="VN496_05310"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HXF16398.1"
                     /translation="MKGILLAGGSGTRLHPVTRVTSKQLLPVYDKPMVYYPLSTLMLA
                     GIRDILVISTPQDLPRFRDLLEDGKKWGIKLSFKEQPRPEGLAQAFIIGRDFVGKDAC
                     ALVLGDNIFYGHDLSARLKRATRPAAGATIFAYQVNDPQRYGVVEFDRAGRAISIEEK
                     PRQPKSRYAVTGLYFYDNTVLDIAASLKPSARGELEITDVNRRYLETGRLRVEMLV"
BASE COUNT         1060 a         1335 c         1363 g          961 t
ORIGIN      
        1 caaagccgac gccgggaact accccacgct cgatgccgtg ggctcgctca ccgacaacta
       61 cgcgagcaac tccgtgatcg gcatccgcag cgacctgcgc tcgctgatcg tcggggtgca
      121 actgaacatc ccgctctacc agggcggggg catcagctcg cgcgtgcgcg aagccgtggc
      181 cggacaagag cgcgcgcgcc aggatctgga aaatgcgcgg cgcaccgtgg cgcagcaaac
      241 ccgcgaagcg ttcctggggg tgacctcggg gctggggcaa atcaaggcgc tgcgccaggc
      301 ggtgggctcg accacgctgc agctcgaatc caccaagctc gggcaggaag tcggggtgcg
      361 caccgcggtg gacgtgctca atgccgagca gcaactggca gccgcgcagc gtgacctggc
      421 acaggcggtc tacaacacca tcctgagcca gttgaaactc aaggcagccg taggcaaact
      481 ggctgaagcc gatctggccg acatcaacgg cctgctcaag gaagacgcga agtaatcccg
      541 gataacgcat gacgaccggg cgcgcagaag cctccgaaga catcgtgcaa gccaaagcag
      601 tattgcgctc acagatcctg gcgcgccgcg atgccaccga cgtagaaact cgaggccagt
      661 attcctcgac cataacagcg aagttgctcg cattgcccgc ttatcgcgca gcgggcgtta
      721 tcgccgctta tgcttctttt ggcagcgagt tcgatacgtc ccggtttttg gccgccgcgc
      781 tggccgaggg aaagcagctg gtattgccga ggataaaccg cgcactacgg gcgctggagc
      841 tacgacaggt tatcgatctg aatggcgatc taatggccgg cgtgtggggg attcctgaac
      901 ccgctccgcg ctgtacgatg gtcgacccga agaagatgga tttcatgctg gtgcctgggg
      961 ttgccttctc acccagcggc gcccgactgg gttacggcgg tggtttttac gatcggctgc
     1021 ttgcctcgct cgattcacag accgtgcgta ttgctgcagc attccaatta caaatggtcg
     1081 ctcaactgcc cgaggcgcct cacgatcaaa gagtccatgc cgtcatcaca gagcagtaat
     1141 tctcgacgca ttaccactcc aggagttcca aagtctttgc cgttgcacca cggtgcgcgt
     1201 tcgtaaagtt cttgccagcg gtggacatcg cttcacgcaa attggcatcg tgcagcagcc
     1261 tctcgattgc tttggccaaa tcctgcgcgt cggtgactcg aattgccgct ccggcttcaa
     1321 ttgcaccgcg cgttgcctcc tcgaaattga aggtgtgtgg gccgacgatg atcggacagc
     1381 ctacggcgca ggcttcgagc agattttgtc cgcccagagg cagcagactg ccaccaacga
     1441 atgcgacgtc gcaggctgcg taataagcaa acatttcacc catcgaatcg ccgatcacga
     1501 cgagcgtatc tccagcgatc ggcgcatttt cgctgcggcg ctgaacttta atgccgcgct
     1561 gtgttatcaa tttgccgacg tcctcgaagc gttgtgggtg acgcggcaca acgactgtta
     1621 acagtcgcgg catatcgatt ttcgacagcg tatccagaat cagcgcttcc tcgccttctc
     1681 ttgtgctggc agccagaaat accggccttt gcgcgccgaa tgcattgcgc aagcgaacgc
     1741 ccaggttcac catttccgtt ggagcaatac ggtcgaactt gatgtttccg gttattgtta
     1801 cgttcctcgc gcccagttcc cgcaagcgct gggcatcctg ctcggtctga gcaccgatat
     1861 gcgagagtct ggacaatgtg ttgccagtca acgctcccat gcggcggtag ccgcgcgctg
     1921 atttagccga catgcgggca ttcaccaaat agagcggtat ggcagactcg gcgctggcat
     1981 gaaccagatt cggccatatt tccgtctcca tgaagatgcc tgctataggg cgaaagtgtt
     2041 gcaggaaacg gcgcgaggcc caggtgaaat cgtaaggaag ataacagcga tccacgcgct
     2101 ctccataaag ctccaaactg gtctggcgtc ccgttggtgt gccgtgcgtc agcaaaattc
     2161 gatgttgggg ataatgtgtt tgcaaggact tgacaagggg ctcggcagcg cgtgtttcgc
     2221 ccaccgagac cgcatgaatc cagatcacgc tgggcgacac gcgatccgga aaaaatccga
     2281 aacgctcgcc aacatgatgc agataggcgg gttgcctgcg cgctctcaac aacagatgca
     2341 gcagcgccca gggcagcagc agatacagcg ccaacgtgta gagcgcgcgg gttaacattt
     2401 ccccggcggg cgcggtatca cgcaggtgac ttcatcgatg ctcagcattg tccgcacact
     2461 ttcaagagca ccgcaaatat atgaggcaga ggtccgcctt ggcagttcga ctcgatgtgg
     2521 gattatccct agaatgacga gctttaaaca gatggcggcg tgatgacgca gaaaagcatc
     2581 attgtcaccg gcggagccgg attcatcggc gctaatttcg tgcagcagtg gatcgctacg
     2641 gagactgccg ccctgctgaa cctggacaaa ttgacctatg cgggcaatct ggccagtctg
     2701 caatcggtcc aatcgaatcc caaatacgat ttcatacgcg gggacatcgg cgactttgac
     2761 ttcgtttccg caaccctggc ggaccgcaag ccaatggccg tggtaaattt cgcggcggaa
     2821 agccacgtgg accggtccat tcacggtcct gcgcaattcg tgcaaaccaa cctggtcggt
     2881 acgtttcaac ttctggaggc ggtgcgcggt tactggtcag ctttagatag cgatagccga
     2941 aaacgctttc ggttgttgca cgtctccact gatgaagtgt atggctcgct gcacgaacac
     3001 gatgcgccat tcaccgaacg aacgccctat gcgcccaaca gcccgtattc cgcgtcgaag
     3061 gcgggctcgg atcacctggt gcgcgcttat caccacacct atggcctgcc tacgattacg
     3121 accaactgct cgaacaatta cggaccctac cagtttcccg aaaaacttat tccgctgatt
     3181 atcctgaatg cgctcgccgg caagccgctg ccaatttacg gcgacggact aaatgtcagg
     3241 gactggttgt atgtcggcga ccattgcacc gcaattcgcc ttgcgctggc caagggccgg
     3301 cccggagaaa cctataacat cggcggcaac agtgaacgca ccaatattga tgttgtgcgg
     3361 acggtctgcc gcatgctcga tgagttgcgt ccggcgccca gcggcaagcg ttacgaatcc
     3421 ttgatcgcat acgtcgccga tcggccgggc catgaccgcc gctacgcgat tgacgccacg
     3481 aaaagcagtg cagaactacg ctgggaaccg accgagacgt ttgaatccgg aatgcgtaag
     3541 accgtgcgct ggtacctgga caatccgcat tgggtgaatg gtgtagtgag cggcgattat
     3601 cggaagtgga tggaaaagaa ttactctgag cggtgagggg caatgagtaa tgggcgatga
     3661 agaacgagat cagaccgcac tataggttag aggcgtggaa agaatcaatc acgctggtga
     3721 aagcgatcta tgaggccacg agaacttttc ctaaagaaga gacctatgga ttggcttcgc
     3781 agatgcgccg agccgcgata tccgttccaa gtaacattgc ggaaggtgcg gcacggactg
     3841 gcagcaggga gttcgcgcag tttctcaacg tttcgagagg ttctttgagt gagttggaga
     3901 cccaactcct catagctgtg gaactgcgat acattgcagc agaccataat gttttcgcgc
     3961 gtctcgacaa ggtatctcgc cttttgacgg gattgcacaa aagcgtcaag gaatgatcat
     4021 gaccaattgc ggattgccca tcgctcatcc ctcattactc atcgcccaag catgaaaggg
     4081 atactactgg caggcggctc gggcacgagg ctgcatcctg tgaccagagt cacctcaaaa
     4141 cagctcttgc ctgtctacga caagccgatg gtgtattacc cgctctcgac ccttatgctg
     4201 gcgggcatca gagacatact cgtcatttca acgcctcagg acctgccgcg tttcagagat
     4261 cttctcgagg atggcaaaaa atggggcatc aaattgtcgt tcaaggaaca accgcgtccg
     4321 gaaggcttgg ctcaggcgtt catcattggt agggatttcg tgggaaagga cgcctgtgcg
     4381 ttggtcctcg gggataatat tttttatggt cacgatctgt ctgcgcgact aaaacgagca
     4441 accaggcctg cggccggagc aactatattc gcttatcaag tcaatgatcc ccagcgttac
     4501 ggggtcgtgg aattcgaccg cgcgggccgg gcgatcagca tcgaggaaaa gcccaggcag
     4561 ccgaagtcgc gctatgcagt aacgggactt tatttttacg acaatacggt actggacata
     4621 gcggcctcgt tgaaaccatc cgcgcgcggt gagctggaaa tcacggacgt caataggcga
     4681 tatcttgaaa ccggccggct acgtgtagag atgctggtc
//
LOCUS       DATMZQ010000161         9959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_129842, whole genome shotgun sequence.
ACCESSION   DATMZQ010000161 DATMZQ010000000
VERSION     DATMZQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129842"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VN496_05315"
     CDS             <1..380
                     /locus_tag="VN496_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF16399.1"
                     /translation="RYNNGEMPKQGAIWLTYYPNVMVEWYPNVLVVSTIVPKDPEHCS
                     NIVEFYYPEDIVLFERKFVEAEQQAYAETAKEDEEICVRMTEGRRALHQQHRNEFGPY
                     QSPMEDGMIHFHEFVRARVQPHL"
     gene            428..1264
                     /locus_tag="VN496_05320"
     CDS             428..1264
                     /locus_tag="VN496_05320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXF16400.1"
                     /translation="MHTTLISVAELAQQVENPNFVIFDCRHELSNPASGSNAYAQSHI
                     PNARFAHVDRDLAAPLTGKNGRHPLPDPDLFGEWLAGMGVSNEKQVVGYDQAGGVYAS
                     RLWWMLRWMGHSAVAVLDGGWQAWLAARLPVTAEVPVPAQGAFTASVQRRFVDANYVL
                     QHLHTLDMILVDARANDRFHGQNETIDPVGGHIPGAVNRFFKDNLTPQGLFKSPQQLR
                     ADFAPLLGNSSPEKIVSQCGSGVTACHNLLAMEIAGLRGGQLYPGSWSEWIAEPSRPR
                     AT"
     gene            complement(1325..3619)
                     /gene="clpA"
                     /locus_tag="VN496_05325"
     CDS             complement(1325..3619)
                     /gene="clpA"
                     /locus_tag="VN496_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HXF16401.1"
                     /translation="MIAQELEVSLHMAFMEARQKRHEFITVEHLLLAMLDNPSAAEVL
                     RACAANVEELRKLLTDFINEHTPVVSGEDVDTQPTLGFQRVIQRAILHVQSSGKKEVT
                     GANVLVAIFGEKDSHAVYFLHQKGVTRLDVVNFISHGITKVPQTAPAKPQAESEADSD
                     QEQSSGGALETYTLNLNALAISGKIDPLIGRERELERVIQTLCRRRKNNPLLVGEAGV
                     GKTAIAEGLARRIIEGEVPEILARCQVYALDMGSLLAGTKYRGDFEQRLKAVLKQLTD
                     NPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGSLKCIGATTYTEYRGIFEKD
                     HALSRRFQKIDVTEPSVAETVEILRGLKTRFEAHHGVKYTASALTSAAELSARFINDR
                     HLPDKAIDVIDEAGAAQRILPKSRQKRVISKHEIEEIIAKIARVPAQSVSSTDRSKLQ
                     NLDRDLKAVVFGQDQAIDALSASIKMSRSGLGNPQKPIGCFLFSGPTGVGKTEVARQL
                     AYTMGVELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEQISKHPYSVVLLDEI
                     EKAHPDIFNILLQVMDHGTLTDNNGRKADFRNTVIVMTTNAGAAELSKTTMGFTQQRA
                     VGDEMVEIKRMFTPEFRNRLDAIISFASLSHEIIVRVVDKFLMQLEEQLHEKKVEVDF
                     TAALKTYLAKNGFDPLMGARPMSRLIQDTIRTALADELLFGKLAGGGSVTVDIDAQGK
                     TKLVFHSSRRETKDSDKNKEESVA"
     gene            complement(3616..3924)
                     /gene="clpS"
                     /locus_tag="VN496_05330"
     CDS             complement(3616..3924)
                     /gene="clpS"
                     /locus_tag="VN496_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HXF16402.1"
                     /translation="MAAGNRDSTLLEAEKSKVKAPPLFKVMLLNDDYTPMDFVVAVLQ
                     TFFSMTREQATQIMLKVHREGMGVCGVYPRDVAATKVEQVIAFSKQHQHPLQCVMEET
                     "
     gene            4214..4417
                     /locus_tag="VN496_05335"
     CDS             4214..4417
                     /locus_tag="VN496_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXF16403.1"
                     /translation="MAIGTVKWFNDAKGYGFITPDDGSEDLFAHFSAIQMNGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAA"
     gene            complement(4480..5721)
                     /gene="icd"
                     /locus_tag="VN496_05340"
     CDS             complement(4480..5721)
                     /gene="icd"
                     /locus_tag="VN496_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXF16404.1"
                     /translation="MYQHIKVPSNGEKITVKKDFSLSVPSNPIIPYIEGDGTGMDITP
                     VMRTVVDAAVRTAYDAKRKIVWMEMFAGEKSCKVYGENVWLPEETLKASREFVVSIKG
                     PLTTPVGGGIRSINVALRQELDLYVCLRPVRYFKGVPSPLKQPEKTDMVIFRENSEDI
                     YAGIEWAAESEDARTLINFLIKEMGVKKIRFPKTSGIGIKPVSREGTERLVRKAIQYA
                     IENQRRSVTLVHKGNIMKFTEGAFRDWGYALAKREFRAEFIDGGPWMKLPGGIIIKDV
                     IADAFLQQILLRPDEYDVIATLNLNGDYISDALAAQVGGIGIAPGANMSDSVAMFEAT
                     HGTAPKYAGKDYVNPGSLILSAEMMLRHMGWIEAADLIVKSMEAAISDKIVTYDFARL
                     MTGATQVSCSGFGNAMVERMR"
     gene            6202..7479
                     /gene="aceA"
                     /locus_tag="VN496_05345"
     CDS             6202..7479
                     /gene="aceA"
                     /locus_tag="VN496_05345"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXF16405.1"
                     /translation="MNRDQAIAAIRKDWKTSARWEGVVRPYSAEDVYRLRGTVRIEYS
                     LAQRGAGKLWNLLHEEAFVNTLGALTGNQAMQQVKAGLKAIYLSGWQVAADANNAGEM
                     YPDQSLYPADSVPSVVRRINNTLIRADQIHHAEGKDGTDWLAPIVADAEAGFGGVLNA
                     FELMKSMIEAGAAGVHFEDQLAAAKKCGHMGGKVLVPTQEAVQKLCAARLASDIMGVP
                     TVLFARTDAEAANLLTSDFDDNDKPFCTGERTAEGYYRVINGIQQAISRGLAYAPYAD
                     LIWCETGKPDLAFARQFAEGIHKKYPGKLLAYNCSPSFNWKKNLDDGTIAKFQRELGA
                     MGYKFQFITLAGFHALNYGMFDLARGYASDHMTAYVKLQQAEFCAEASGYTATKHQRE
                     VGTGYFDEITQAVQGGKSSVTALKGSTEEEQFV"
     gene            7617..9206
                     /gene="aceB"
                     /locus_tag="VN496_05350"
     CDS             7617..9206
                     /gene="aceB"
                     /locus_tag="VN496_05350"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HXF16406.1"
                     /translation="MQVNGPITQEHATVLTGEALAFLVKLHRNFDARRIELLARRMQR
                     QKEIDSGKLPDFLVETKAIRDAAWQVAPVPQDLRDRRVEITGPTDRKMVINALNSGAN
                     MFMADFEDSSTPTWDNQLQGQINMRDALSGSISFTSPEGKHYKLSDKPATLLVRPRGW
                     HLIEKHILIDGEPMSGSLFDFGLYFFHNAFERVKKKSGIYLYLPKMESHLEARLWNDV
                     FVFSQQELGVPQGTVKATVLIETILAAFEMEEILFELRAHSAGLNCGRWDYIFSCIKK
                     FRNHKEFILADRGLVTMTSPFMRAYSLLLIKTCHKRGAFAMGGMAAQIPIKNDSAAND
                     AALAKVRADKEREANDGHDGTWVAHPGLVSIAKEAFNRAMKGPNQIDKKRDDVNVTAT
                     DLTDFQPKSPITEQGLRININIGIQYLGAWLAGTGCVPIFNLMEDAATAEISRAQIWH
                     WIRSARGVMEDGRKVSKELFREFVPQELAKVRDMLGEKGYAAGKYEEGAKMFEQLTLA
                     DEFVEFLTLPAYEYIIAHEHR"
     gene            9203..9778
                     /locus_tag="VN496_05355"
     CDS             9203..9778
                     /locus_tag="VN496_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXF16407.1"
                     /translation="MNRSAGSGRADVKIVLFNKPYGVICQFSPSEGKRSLKEFVPLRD
                     VYPAGRLDTDSEGLLLLTNDGALQNEVSHPGKKWPKSYWVQVENCPHEAAMAKLRDGV
                     DLGDFVSAPAVVQEIAEPANLWPRSPPIRVRRAIPTCWLDITITEGKNRQIRRMTAAV
                     GSPTLRLIRYRIGPWTLYGLAPGTYRVLAST"
BASE COUNT         2285 a         2701 c         2682 g         2291 t
ORIGIN      
        1 tgcgttataa caatggcgag atgcccaaac aaggcgcgat ctggctcact tattatccaa
       61 atgtgatggt cgagtggtat ccgaacgtgc tggtggtcag tacgatcgtt ccgaaagatc
      121 cggaacattg cagcaatatt gtcgaattct attatcccga ggatattgtg ctgttcgagc
      181 ggaagttcgt tgaggcagaa caacaggcct atgccgagac agcaaaagaa gatgaagaga
      241 tctgcgtacg catgacggaa ggccgccgcg cgctgcatca gcaacatcgc aatgaatttg
      301 gtccttatca gtcaccgatg gaagatggca tgatccattt tcatgagttt gtgcgtgcgc
      361 gcgtgcaacc gcatctctag tcttcgtgct gctggtacca gccagcattc acgaacgggc
      421 tgagaccatg cataccacgc tgatctccgt tgccgaactc gcgcagcaag tcgaaaatcc
      481 caactttgta attttcgatt gccggcatga gttgagcaac cctgcatcgg gctcaaacgc
      541 gtacgcgcaa tcgcatatcc cgaacgcccg ttttgcccat gttgaccgcg acctcgcggc
      601 gccacttact ggaaaaaacg gacgccatcc attgcctgat ccggacttgt tcggcgaatg
      661 gttggcgggt atgggagtat caaacgaaaa gcaggtagtc ggttacgacc aggctggagg
      721 ggtatacgcg tcccgactat ggtggatgct gcgctggatg ggccattccg cggtggccgt
      781 tctcgatggt ggctggcagg catggctcgc tgcgcgattg ccggttaccg cggaagttcc
      841 tgtaccggca caaggagcat tcaccgcttc ggttcagcgc cgttttgtgg atgcaaacta
      901 tgtgctgcag cacttgcata cgctagacat gattctggtt gatgcgcgtg caaacgatcg
      961 atttcacggc cagaacgaga ctatcgatcc agtcggcggt cacattccag gagccgtaaa
     1021 ccggtttttc aaagataacc tgacaccgca gggtttgttc aaatcgccgc agcaattacg
     1081 cgccgatttc gcaccgttgc tcggaaacag ttcgccggaa aaaattgtca gccaatgcgg
     1141 gtcgggtgta acggcttgcc ataatctgct ggcgatggaa atcgcgggat tgcgcggcgg
     1201 gcaactctat cccgggtcct ggagtgaatg gatagccgag ccgtccaggc cgcgggcaac
     1261 ttgacacgca attcgtagcg caaaaaaacc ggaccttaag tccggttttt ttatctatgc
     1321 cttctcaggc gacgctttcc tctttgtttt tgtcgctatc cttggtctcg cgcctggagg
     1381 aatggaacac caacttcgtc ttgccttggg catcgatatc gaccgtaaca ctgccgccac
     1441 cagccagttt gccgaacaac aattcgtcgg cgagcgccgt gcgtatggtg tcctggatca
     1501 aacgtgacat tgggcgcgcg cccatcaggg gatcgaaacc gtttttagcc agataggtct
     1561 tcagcgcagc ggtgaaatcg acctccactt ttttctcgtg taactgttct tcaagctgca
     1621 tcaggaattt gtccaccacg cgcacgatga tctcatgcga cagtgatgca aacgaaatga
     1681 tggcatcgag ccgatttcga aattccggcg taaacatgcg cttgatttcg accatctcgt
     1741 ccccaaccgc ccgctgctgg gtgaagccca tcgttgtttt cgacagttca gcggcgccgg
     1801 cattggtagt catcacgatc accgtgttgc ggaagtccgc cttgcgtccg ttgttgtcgg
     1861 tcaacgtccc gtgatccatc acctgcaaaa gaatattgaa aatatccggg tgtgccttct
     1921 cgatctcgtc aagcagcacg accgagtacg gatgcttgga aatctgctcg gttagcagcc
     1981 ctccttgatc aaaaccgacg taaccgggag gcgcgccaat cagccgggac accgcatgcc
     2041 gctccatgta ttcggacata tcgaatcgga tcagctccac gcccatggta taggccaatt
     2101 ggcgggctac ttcagtcttg ccaacaccgg ttgggccgct gaagagaaag cacccgatcg
     2161 gtttttgcgg atttcccaga ccgctgcgcg acatcttgat ggaggccgac agggcatcga
     2221 tggcttgatc ctgcccgaat acgacagcct tcaagtcgcg atccagattc tgcaacttgc
     2281 tgcgatccgt ggacgacacg ctttgcgcag gaacgcgcgc aatcttggcg atgatttcct
     2341 caatctcgtg tttgctgata acccgcttct gcctcgattt gggcaggatg cgctgagccg
     2401 ccccggcctc gtcaatcaca tcgattgcct tgtctggcag atgccggtca ttgatgaagc
     2461 gcgcggaaag ctcggctgct gaagtaagcg ctgatgcggt gtacttcacg ccatgatgcg
     2521 cttcaaacct ggtcttcagg ccgcgcaaga tctctaccgt ctctgccacc gacggttcgg
     2581 tcacgtctat cttttggaaa cggcgcgaca gcgcgtggtc tttttcaaat attccacggt
     2641 actcggtata agtggttgca ccgatacatt tgaggctgcc tgacgaaagc gccggtttga
     2701 gcaagttcga ggcgtccagc gttcccccgg atgcggcacc tgcgccgatc agggtatgga
     2761 tttcgtcaat gaacagaatt gcgttcggat tatcggtgag ctgcttcaga accgctttaa
     2821 gccgctgctc gaaatctccg cgatacttgg ttcccgctaa tagcgagccc atgtccaacg
     2881 cgtaaacctg gcaacgcgcg agaatttctg gcacttcccc ttcgataatg cgccgcgcca
     2941 acccttcggc aatcgcggtt ttgcccactc ctgcctctcc cacgagtaac gggttgtttt
     3001 tacgccgacg acacaaggtc tggataacgc gctccaattc gcgctcacgt ccgatcaatg
     3061 gatcgatctt gccggagatg gcaagcgcat tgaggttgag cgtataggtc tccagcgcgc
     3121 caccggaact ttgttcctgg tccgaatctg cttcgctttc agcctgtggc ttggccggcg
     3181 ctgtctgcgg aactttagtg atgccatggg atatgaagtt cactacgtct agccgcgtca
     3241 cgcctttctg gtgcagaaag tagactgcgt gcgaatcttt ctcgccaaag atggcgacca
     3301 gcacgtttgc gcccgtaact tctttcttgc cagacgactg aacgtgaagg atcgcacgct
     3361 gaatcacgcg ctggaagccc agcgtgggct gcgtatcgac atcctcgccg ctgacaaccg
     3421 gtgtatgttc atttatgaaa tcggtgagca gcttgcggag ttcctccaca ttggccgcac
     3481 acgcgcgtaa gacctcggcc gccgacgggt tgtccagcat ggcaagcaac agatgctcca
     3541 ccgtaatgaa ctcgtgccgc ttctgcctag cctccatgaa cgccatgtgc aaactgactt
     3601 ctagttcctg cgcaatcatg tttcctccat cacgcactga agcggatgtt gatgctgttt
     3661 tgaaaacgca atgacttgct ccaccttggt cgccgccacg tctctaggat acacgccaca
     3721 gacgcccatg ccttccctgt gaactttgag catgatttgg gtcgcttgct ccctagtcat
     3781 cgaaaaaaaa gtctgtaaca ctgcgacgac aaaatccatc ggcgtgtaat catcgttcag
     3841 cagcataacc ttgaacaatg gtggcgcctt gaccttgctc ttctcggcct ctaggagcgt
     3901 gctgtcacga tttcccgctg ccatgctctc gtctcgcttc gctgctttca ccaactctaa
     3961 ttttgcggat aacggtgaaa aacacaagtg cagaattcat gcccgaggcg ctcagccgat
     4021 cggctaaatc tgcattgcaa caaccggttg cgcgcgttcg gcatacttga cttcacaaaa
     4081 caaataacct acaaagcgaa ctggtgtttg gcttcaagca tactgcaggt accggcgttg
     4141 ccgataagcg cggacttgaa actcaaacgg tcagcgggta tctggcccgc gttcttcaac
     4201 gagggaagta tgtatggcaa tcggtacggt caagtggttc aacgacgcaa aaggctacgg
     4261 cttcatcaca cctgatgacg gaagcgaaga tctgtttgcg catttttcgg cgattcagat
     4321 gaacggcttc aaaacgctga aagagggcca gaaggtaagt tttgaggtca cccaggggcc
     4381 caaaggcaaa caggcatcaa atatccaggc cgcctgacgc ggcgacaccc aatattgaaa
     4441 gcccgcgacg tttcgcgggc ttttttgtct aatgcgtgat caacgcatgc gttccaccat
     4501 agcattgccg aagcccgagc acgaaacctg tgtcgcgccg gtcatcagcc tggcaaaatc
     4561 gtaagtcacg attttgtcgc tgattgccgc ctccatggat ttcacgatga ggtccgccgc
     4621 ttctatccat cccatgtgac gtaacatcat ctcggcagat agaatcaggg aacccggatt
     4681 cacatagtcc tttccggcgt actttggcgc ggtgccatga gtagcctcaa acatcgcaac
     4741 agaatcgctc atgttcgcac ccggtgctat tcctataccg cccacctggg cagccagggc
     4801 gtctgaaata tagtcgccgt tcaagttcaa tgttgcgatt acgtcgtatt cgtcgggacg
     4861 tagcagtatc tgttgcagga aggcatctgc gatcacgtct ttgatgatga tgccgccagg
     4921 tagtttcatc cacggtccgc catcaataaa ctcggcccgg aattcgcgct tcgccagtgc
     4981 atagccccaa tcgcgaaagg cgccttcggt gaatttcatg atattgccct tatgcaccag
     5041 ggtcaccgat cgccgctgat tttcaattgc gtactgtatc gccttgcgca cgaggcgttc
     5101 ggtgccctcc cgggaaaccg gcttgatgcc gatccctgaa gtcttgggaa atctgatctt
     5161 cttgactccc atttccttga ttaaaaagtt gatcagcgtg cgggcgtcct ccgactcagc
     5221 agcccattcg atgccggcat aaatgtcttc cgagttttcc cgaaaaataa ccatgtcggt
     5281 tttttccggc tgcttgagcg ggctcggcac ccctttgaaa taccgcactg gtcgcaaaca
     5341 gacatatagg tcgagctcct ggcgcaaagc aacgttgata gaacgtattc ctcccccgac
     5401 cggggtagtc aaaggaccct tgatggagac aacgaattcc ctgctggcct tgagcgtttc
     5461 ttccggtagc cacacgtttt ccccgtaaac cttgcaggat ttttctcctg caaacatctc
     5521 catccataca atcttgcgct ttgcgtcata ggctgtacgg accgcggcat caaccaccgt
     5581 acgcatcacg ggcgtgatat ccataccggt tccgtcaccc tcgatgtagg gaatgatcgg
     5641 gttcgacggg acgctcagcg aaaaatcctt ttttaccgtg attttttcgc cattggacgg
     5701 cactttgatg tgctgataca tgggtcttcc tcgatagtcg atgttgcgaa atttggacgt
     5761 gctggattgg atggctaagc aaaaagacgg caattgtaag gcaacgactg gacccggcac
     5821 aaaagcaact ggctcttctg caccgaacgc cattttttta aaattaattt tcgcattgca
     5881 aaatgatgcg caactcaaaa taccaatctt ttgattatca agcacttttt tgcttcgggt
     5941 acgtttgttg caacgcgtaa aaatagtact gtaccttttg tgcaccaccg ttgcatttga
     6001 tccaatccgg agtgcaccga gcagatctgg gatgccgcca aacgcgtcat ctttgtgagc
     6061 cggccaggcg gaatgtctgg ctcttcacgt ggcaagtggc cgcgccgaag ttaggaagca
     6121 gcgcgcgcaa tttacgattt gaagtccggg aagccttgcg cgacggactg taagttctgg
     6181 gttaacgaat aggagaccgc catgaatcgc gatcaggcaa tcgcagcaat cagaaaagac
     6241 tggaaaacga gtgcacgttg ggagggtgtc gtacgaccct attccgccga ggacgtctat
     6301 cgccttcggg gaaccgtgag aatcgaatac tcgcttgcac agcgtggagc cggcaagctt
     6361 tggaatcttc tgcacgaaga ggcatttgtt aatactttgg gtgccctgac cggaaaccag
     6421 gcgatgcagc aagtcaaggc aggattaaaa gccatttacc tgtctggctg gcaagtggcg
     6481 gcggatgcga acaatgccgg cgaaatgtat ccagaccagt ctctctatcc tgccgacagc
     6541 gttcccagcg tcgtacgccg cataaacaac actttaattc gggcagacca aatacaccac
     6601 gcggaaggaa aggatggaac cgactggctg gcacccattg ttgcggatgc tgaagcgggg
     6661 ttcggtggcg tgcttaacgc gtttgaactg atgaagtcga tgatagaagc gggcgctgcg
     6721 ggagtccact ttgaagacca gcttgccgct gcgaagaaat gcggccacat gggcggaaaa
     6781 gtcttggttc cgactcaaga ggcagtacag aagctgtgcg ctgcgcgcct ggcttcggac
     6841 atcatgggtg taccgaccgt gttgtttgct cggaccgatg cggaggccgc caatctcctc
     6901 acctcggatt tcgacgacaa cgacaaacca ttttgcaccg gcgagcgcac cgctgaaggc
     6961 tactatcgtg taatcaacgg catccagcag gcgatttcgc gcggtctggc gtatgcaccc
     7021 tatgctgatt tgatttggtg tgagacaggc aagccggatc ttgcattcgc aaggcaattc
     7081 gccgagggca tacacaagaa atatcccggc aaactgctcg cgtacaattg ttcaccgtcg
     7141 ttcaattgga agaagaatct tgacgacggc accattgcaa aattccaacg ggaacttggt
     7201 gcaatgggct acaagttcca gttcatcacg ctggcgggct ttcacgcact caattacggg
     7261 atgttcgatt tggcgcgcgg gtacgcgagc gaccatatga ccgcatacgt aaaactgcag
     7321 caggctgaat tctgtgccga ggctagcggt tacaccgcaa cgaagcacca gcgggaagtt
     7381 ggaaccggtt atttcgacga aatcacgcag gcggtacagg gaggcaagtc gtcggttacg
     7441 gccctcaaag gatccactga agaagaacaa ttcgtctgaa aaagtattgg ggtctggcgc
     7501 cgcgttggca ggcaatgctt gttcacgcgg cgtttcctat ttatcatttt gcgaaattgt
     7561 ttttcacaat atgaaataga ggaccaaacg tggaccagtt aaaaatcccg gccggcatgc
     7621 aggtaaatgg accgatcacg caggaacatg ccactgttct gaccggcgaa gcgcttgcgt
     7681 ttttggtgaa attgcatcgc aacttcgacg cgcgccggat cgaattgctc gcgcgccgga
     7741 tgcaacggca aaaagagatc gactcgggca agttgcctga ttttcttgtc gagaccaagg
     7801 ctatccggga tgcggcctgg caagtagcac cggttcctca ggatttgcgg gaccgtcgcg
     7861 ttgaaatcac tggtccgacg gatcgcaaga tggtcatcaa cgcgctaaat tccggcgcga
     7921 atatgttcat ggccgatttc gaggattcat cgacgccaac ctgggacaac cagctgcaag
     7981 ggcaaatcaa tatgcgcgac gccctgtccg gcagcatttc attcaccagt ccggaaggta
     8041 agcactacaa gctttcagac aaaccggcga cccttctggt aagaccccgc ggatggcatc
     8101 tcatcgaaaa gcacattctg atcgatggcg agcccatgtc cggatcgttg ttcgatttcg
     8161 gcctgtactt ttttcataac gcattcgaac gggtaaagaa aaagtccggc atttacctat
     8221 atctgccgaa gatggagtcg catctcgagg cgcgcctatg gaacgatgtc ttcgtattct
     8281 cacaacagga actgggcgtc ccgcagggaa cggttaaggc gactgtcctg atcgagacca
     8341 tcctcgccgc gttcgaaatg gaagaaattc tattcgagtt gcgtgctcat tcagctggcc
     8401 tgaactgcgg acgctgggat tatattttca gttgcatcaa gaaatttcgt aaccacaagg
     8461 agttcatact cgctgatcgc ggtcttgtga ccatgacaag tccattcatg cgggcctatt
     8521 ccctgctgct gatcaagact tgccacaagc gcggtgcatt tgccatggga ggcatggcag
     8581 cgcagattcc gatcaagaac gattcggccg caaacgatgc ggccttggcg aaagtgcgtg
     8641 ctgacaagga gcgtgaagcg aatgatggcc atgacggcac ttgggtcgcg catcctggtt
     8701 tggtatcaat cgccaaggaa gcgttcaaca gggcaatgaa aggacccaac caaatcgata
     8761 agaagcggga tgacgtcaat gttactgcca cagatctgac cgatttccag ccgaagagcc
     8821 ctatcacgga gcaggggctt cgtatcaata tcaacattgg catccaatac ctgggcgcgt
     8881 ggctcgccgg gaccggttgc gtgcctattt tcaatttgat ggaagatgcg gccactgccg
     8941 aaatttcccg cgcgcagatc tggcactgga ttcgcagcgc tcgcggcgtg atggaagacg
     9001 gccgcaaagt gagcaaggaa ttgttccgtg agttcgtacc tcaagaatta gcaaaagtgc
     9061 gcgacatgct gggagaaaaa ggctacgcag ccggcaagta cgaggagggg gcgaaaatgt
     9121 tcgagcagct tacgctggcg gacgaattcg ttgaattcct gactctgcca gcctacgaat
     9181 acatcattgc ccacgaacac cgttgaatcg ttctgcggga tctggcaggg cggatgtcaa
     9241 aatcgtcctg ttcaacaagc cgtatggcgt tatctgccag ttcagccctt ccgaaggcaa
     9301 gcgttcgctc aaagaatttg ttccgctgag ggacgtctac cctgctggca ggcttgatac
     9361 agatagcgaa ggcctgctgc tgctaaccaa cgatggcgcc ttgcagaacg aggtaagcca
     9421 ccccgggaag aaatggccaa agagttattg ggtccaggtg gaaaactgcc cccatgaggc
     9481 ggcgatggca aaactccgcg acggggtgga tcttggagat tttgtttcgg ctcctgccgt
     9541 cgttcaagag attgcggaac ccgcgaactt gtggccgcgt agtccgccta ttcgcgtgcg
     9601 cagggccatt ccaacatgct ggctggatat caccatcact gaaggcaaaa accgccagat
     9661 tcgcagaatg actgcagcgg tcggttcgcc caccctgcgc ctgatccggt accgcatcgg
     9721 tccatggacg ttatacggac ttgcgccggg gacttaccgg gtactggcgt ccacgtaaac
     9781 cccgttctcc cgggcactat acttgtaacc aaatatgtca accgtgtagc aatgggggcg
     9841 ttaaaggcca tgacacggac ttgtgtcacc tgaagaaccc actattggag aagacgcatg
     9901 aagaagattg tcgccgcgct gatcgctgcg ctgtttgccg ctgtaacgtt ctcggcggt
//
LOCUS       DATMZQ010000162         3722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4900198, whole genome shotgun sequence.
ACCESSION   DATMZQ010000162 DATMZQ010000000
VERSION     DATMZQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4900198"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VN496_05360"
     CDS             <1..1065
                     /locus_tag="VN496_05360"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXF16408.1"
                     /translation="NRLKSKPASERKSLKRVIFGFLRESKGIHSALERGVAIGEGVNF
                     ARDLGNLPPNICTPGYLAEQARSLGKTHKMKVTVLERADMKKLGMGSLLSVSDGSRQP
                     PKLITMEYYGGPKKQKPVVLVGKGVTFDTGGISLKPGAEMDEMKYDMSGAGSVLGTMS
                     AVAAMKLPLNVVGVMPATENMPGGNATRPGDIVTSMSGQTIEILNTDAEGRLILCDAL
                     SYSERFEPAAVIDIATLTGACVIALGHVASGLFANDEQLAEEVTAAGVAAWDRVWPMP
                     VWQDYQEQLKSNFADMANIGGRPAGSITAACFLARFTKKFKWVHLDIAGTAWKSGREK
                     GSTGRPVPLLTQFLMTRAGW"
     gene            1062..1490
                     /locus_tag="VN496_05365"
     CDS             1062..1490
                     /locus_tag="VN496_05365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HXF16409.1"
                     /translation="MTEVLFYTHADDKLRMACMLTGKAFARGMRVMLFTPDSSTTEHL
                     TKLLWTLPATGFLPHCRTADRLAAVTPVIVDHVSDPLVHNDVLLNLCDATPPFFSRFR
                     RLVEIVGPDASDREAARVRYRFYRDRGYDIRTHQIAAPLS"
     gene            1490..1957
                     /locus_tag="VN496_05370"
     CDS             1490..1957
                     /locus_tag="VN496_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16410.1"
                     /translation="MAENIPPNEGPAPDPGEEGDVVGKIDQLLHRHRYKLPSDGSMPG
                     LADPSRVPEAPIADGIPVLTDVVAGPGHDAKLPPVPSRSATINSVLIVRRMALALDAE
                     HARLSAAMGGDVNQLKMLDHLVAELKHALPAAVRAAIIDGAPGSFRQRDDGQL"
     gene            1985..>3722
                     /locus_tag="VN496_05375"
     CDS             1985..>3722
                     /locus_tag="VN496_05375"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXF16411.1"
                     /translation="MELAKSFEPKEIEQRWYPVWESRGYFEVHGDQNRPAYCIQLPPP
                     NVTGTLHMGHAFQQTLMDILIRYHRMRGFATNWVIGTDHAGIATQIVVERQLESEGKT
                     RSDLGREQFLERVWAWKQQSGSTITRQMRRLGASGNWSFADTEDQNSGYFTMDAKMSR
                     AVVEVFVRMHEEGLIYRGKRLVNWDPVLGTAVSDLEVDSEEEAGRIWEIRYPLAEGDS
                     SLVVATTRPETTLGDVAVAVHPEDDRYRALVGKQVQLPLSGRNIPIIADEYVDPLFGT
                     GCVKITPAHDFNDYVVGNRHGLPLINILTLDGKINDEAPLPYRGLERFEARKRVLADL
                     RAQSLLVSEKPHRLKVPRSGRTGVIVEPMLTDQWFMRMEGLAARALEVVANGDVRFYP
                     EHWTATYNHWLENIQDWCISRQLWWGHQIPAWYDDNGNVYVGRSESEVRQKYASQLAP
                     QASLRRDEDVLDTWFSSALVPFSSLGWPDQTRELQKFLPSSVLVTGFDIIFFWVARMI
                     MMTLHFTGQVPFRDVYINAIVRDAEGQKMSKSKGNTIDPLDLIDGIGLEELVAKSTVG
                     LLRAEHKQKIEKT"
BASE COUNT          836 a         1022 c         1097 g          767 t
ORIGIN      
        1 aatcgtctga agagcaagcc cgccagtgag cgcaagtccc tgaagcgagt cattttcggg
       61 ttcctgcgcg aatccaaggg aatccacagc gcgctcgaac gtggggttgc cataggtgaa
      121 ggcgtcaatt ttgcgcgcga cctgggcaac cttccgccaa acatctgcac accgggttac
      181 cttgccgagc aggctcgttc gctgggcaag acgcacaaga tgaaagtaac ggtgctcgaa
      241 cgcgccgaca tgaaaaaact cggtatggga tccctgttat cggtttccga cggaagccga
      301 caaccgccaa aactcatcac catggaatat tacggcggac cgaaaaagca aaagcctgtc
      361 gtgctggtgg gcaaaggcgt tactttcgac actggcggca tttccctgaa accgggcgcc
      421 gaaatggatg aaatgaaata cgacatgtcg ggcgcgggca gcgtactcgg cacgatgagt
      481 gcggtggccg cgatgaagct gccactcaat gtagttggcg taatgcccgc cacggaaaac
      541 atgccaggcg gcaatgccac ccggccgggt gacatcgtca caagtatgtc agggcaaacg
      601 attgagattt tgaatacgga cgcggaaggt cggttgattc tgtgcgacgc gttatcctat
      661 tccgaacgtt ttgagcctgc tgctgtaatt gatatcgcaa cgctaactgg cgcatgcgtg
      721 attgctctcg ggcatgtggc ttcaggtttg ttcgccaacg acgaacaact tgctgaggaa
      781 gtcactgcgg ctggagtcgc ggcatgggac cgcgtgtggc ccatgccggt gtggcaagac
      841 taccaggagc aactgaaaag caattttgct gacatggcca acatcggcgg gcggccggcc
      901 ggcagcataa ctgccgcctg ttttctggcg cgttttacca aaaaatttaa gtgggttcat
      961 ttggacattg ctggcaccgc atggaaatcg ggccgcgaaa aaggttctac aggccgtcca
     1021 gtgccgctgc tgacgcagtt tctcatgacg cgcgccggct ggtgaccgaa gtcctgttct
     1081 acacccacgc cgacgacaag ctgcggatgg cttgcatgct gaccggcaag gcatttgcgc
     1141 gcggcatgcg cgtaatgctt tttacgccgg actcttctac caccgagcat ctcaccaaat
     1201 tgctgtggac gctgcccgcc accgggttct tgccccattg tcgtaccgcc gaccggctcg
     1261 ccgcggtaac accagtcatt gtcgaccatg tttcagaccc gctggttcac aatgatgtcc
     1321 tgcttaatct ttgcgatgct acgccaccgt tctttagccg atttcgccgg ctggtggaaa
     1381 ttgtcgggcc cgacgcaagc gatcgtgaag cggcacgcgt gcgttaccgc ttttaccgcg
     1441 accgcggtta cgacatacgc acccaccaga tcgcagcacc actgtcctga tggctgaaaa
     1501 tattccgccg aatgaagggc cggctcctga tcccggcgag gaaggcgacg tggtgggcaa
     1561 gatcgatcaa ttgctccatc gccatcgtta caagttacct tctgacggca gtatgccggg
     1621 attggcggat ccctcgcgcg taccagaggc gccgatcgcc gatggcattc cggtccttac
     1681 tgacgtggtc gcggggcccg gccacgacgc aaagctgccg cccgtgccgt cccggtccgc
     1741 aacgatcaac agcgtgctaa tcgtgcgacg catggcattg gcgctcgatg ccgagcatgc
     1801 gagattatcg gcggcgatgg gaggagatgt taatcagttg aagatgctgg accatcttgt
     1861 tgccgagctg aaacatgcgt tgccagcggc cgtccgtgca gcaatcatcg acggagcacc
     1921 gggttctttt cggcagcgcg acgacggtca gctataattt gcggccggat ttttgatgtt
     1981 gaaaatggaa ctcgcaaaga gcttcgagcc gaaggagatc gagcaacgct ggtacccggt
     2041 ttgggaatcg cgcggctact tcgaagtgca cggtgatcag aacaggccgg cgtattgcat
     2101 tcagcttccg ccaccaaatg taaccggcac attgcatatg ggtcacgcgt tccagcagac
     2161 cctcatggat attttgattc gttaccaccg catgcggggc ttcgcgacca actgggtgat
     2221 cgggacggat cacgccggca tcgcgacaca gatcgtggtc gagcgccaac tcgaatcgga
     2281 aggcaagacg agaagcgatc tgggccgtga acagtttctt gaacgggttt gggcatggaa
     2341 acaacagtcc ggctccacca ttacgcggca aatgcgccgc ctcggcgcct ctggcaactg
     2401 gagcttcgcc gatacggaag accagaacag cggctacttc acgatggatg caaagatgtc
     2461 ccgcgccgtg gtcgaagtat tcgtgcgtat gcatgaagaa gggctcatat atcgcggcaa
     2521 gcggttagtg aactgggacc cggtactcgg aaccgcagta tccgatctgg aagtcgatag
     2581 cgaagaggaa gcaggccgca tttgggaaat tcgttatcca ttggccgaag gggacagcag
     2641 cttggtcgta gccacaacac ggcccgaaac cacgcttggg gacgtcgccg ttgcggtgca
     2701 tccagaagat gatcgctatc gagcgctggt gggaaagcaa gtgcagttgc cgctgagcgg
     2761 gcggaacatt ccaatcattg ccgacgagta tgtcgatcct ctgttcggca ctggatgcgt
     2821 gaaaattacg ccagcccacg atttcaatga ctacgtcgtt ggaaatcggc acgggcttcc
     2881 gctaatcaat atcctgaccc ttgatggaaa gatcaacgat gaagcacctc tgccctatcg
     2941 cgggctggag cgattcgaag cgcgcaagcg cgtcctggcc gacctgcgcg cgcaaagcct
     3001 gctcgtttca gaaaaaccgc acagactaaa ggtgcctcgc tcaggccgga ctggtgtgat
     3061 cgttgagcca atgctcaccg accagtggtt catgcgaatg gaaggtctcg cagcacgggc
     3121 attggaggtc gtggcaaacg gcgacgttag gttttaccca gaacactgga cggctactta
     3181 caaccattgg ctggaaaaca tccaggattg gtgtatctcc cggcaattgt ggtgggggca
     3241 tcagattccg gcgtggtatg acgataatgg caacgtttat gtcggccgca gcgaatcgga
     3301 agtcaggcaa aaatatgcct ctcaactggc tccgcaggct tcgcttagac gggatgaaga
     3361 cgtgctggac acctggttct cgtcagcgct cgtgccgttt tcttcgctgg gctggccgga
     3421 ccaaacccgc gaattgcaga aatttcttcc gtcctcggta ctggtcaccg gctttgacat
     3481 catcttcttc tgggtggcgc ggatgatcat gatgacattg catttcaccg gccaggtgcc
     3541 tttccgcgat gtgtacatca atgccatcgt gcgcgatgcc gaagggcaaa aaatgtccaa
     3601 gtccaagggc aataccatcg atccactgga cctcatcgat ggcattgggt tggaagagct
     3661 ggtggccaaa tccacggtgg gactgctgcg ggctgaacac aagcaaaaaa tagaaaaaac
     3721 ca
//
LOCUS       DATMZQ010000163         2698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4554489, whole genome shotgun sequence.
ACCESSION   DATMZQ010000163 DATMZQ010000000
VERSION     DATMZQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554489"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VN496_05380"
     CDS             <1..364
                     /locus_tag="VN496_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXF16412.1"
                     /translation="SDPRVTSFGAILRRTSLDELPQFFNVFQGHMSVVGPRPHAVAHN
                     EQYRKLVPGYMLRHKVKPGITGWAQVNGWRGETDTVEKMQRRVDCDLYYIRNWSILLD
                     IKIVVLTIIRGFRQPSAY"
     gene            complement(422..2155)
                     /locus_tag="VN496_05385"
     CDS             complement(422..2155)
                     /locus_tag="VN496_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HXF16413.1"
                     /translation="MKLDESLHRNDPQEKSGATHIGRWYLAGLFVLALGLRCVGLEWG
                     GRHPDENIGDAARALTGELVPLQPFYPPLLNYLTAAGDVLLFLIGLPAGLWSNLTGFR
                     DAYFETPEIFLLVLRLVVSVTGALAAPLSAMIAGRLALSRAGCLAVGVAMALLPLSVW
                     RSHIGKPDLGVAASALLAIWAMLRYAQELRRADAAWMGIAFALAISFKHSALFIFAPF
                     CLAVLVMARQSGQTWPRLMRDALVAFGVGLAVWVPANVGLWLDLSNFLDYQKVQAAMS
                     ERDSGILLTAATVSALLSNTFAGPTFPLLVAFLGAPLCWRRLDVLAIWTSVIASIVAV
                     AAITGERAPIQLYVPQSVLIAGIGMITWVALAQRTGPIRRLGMTGMIVTAIVLGMGTF
                     DVVRQALLPDIRSRVSQVLLGIEGIAHRRIMISEVQYAGLPVSGDAERDERARHERLA
                     LQYGVNLPPRRRPYAPVEGRYYIRRFPSVMGGLEIYEEKDVKLVKPFTWPLQPTEWKL
                     DYWLDQGYSVFVVLNEERVLKSKVDAYRLFHEEIRDRGVLLATVEANRTLFQESSAKV
                     YLVDPAAKTGK"
     gene            2369..>2698
                     /locus_tag="VN496_05390"
     CDS             2369..>2698
                     /locus_tag="VN496_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXF16414.1"
                     /translation="MKLLAQALRLHQWAKNMLLAVPLLTAHLVLDLEAWTSVIVAFFG
                     FGFVASATYLVNDLIDLKSDRLHAQKRFRPMASGRLTVSAGMALAIVLAFLGFAISAA
                     ALPAGFIH"
BASE COUNT          558 a          826 c          786 g          528 t
ORIGIN      
        1 gtcggacccg cgcgtgacga gctttggagc catcttgcga cgcacgtcat tggacgaatt
       61 gccgcaattt ttcaatgttt tccaggggca tatgtctgtg gtcggccctc gcccgcatgc
      121 cgtggcgcac aacgagcagt accgaaaact cgtcccaggg tacatgctca ggcacaaggt
      181 gaagccgggc attacgggat gggcccaggt gaacggctgg cgtggcgaaa cagatactgt
      241 cgagaagatg caaaggcgcg tcgactgcga tctgtactac atccgcaatt ggtcgatttt
      301 gttggacatt aagatcgttg ttctcacgat aattcgcggc ttcaggcagc cgagcgcgta
      361 ctgacccgaa ccactcacag tacacctgcc tatgggccga aggccgttgc acgcgctact
      421 gctatttgcc ggtttttgcc gccggatcga ccaaatacac cttggcgctg ctttcctgga
      481 aaagcgtcct gttggcttca accgtagcga gcaggacgcc gcgatcgcgg atttcttcat
      541 ggaataaccg gtaggcgtcc actttcgatt tcagcacgcg ctcctcattc aagacgacga
      601 atacgctgta tccctgatcg agccagtagt ccagtttcca ctccgtcggc tgcagcggcc
      661 aggtaaacgg cttcacgagc ttgacgtctt tttcctcgta aatctccaat ccacccatca
      721 cggaaggaaa gcgccttatg taataccgac cttcaaccgg cgcgtagggc cggcgacgag
      781 gcggcagatt tacgccgtat tgcagtgcca gccgctcgtg tcgcgcacgc tcatcccgct
      841 cagcatcgcc gctcaccggc agtcccgcat actggacttc gctaatcatg atccggcgat
      901 gcgctatgcc ttcgatgccg agcaggacct gggaaacccg gctgcggata tcgggcaaga
      961 gcgcctgccg tacgacgtcg aaggtaccca ttccaagcac gatcgcggtc acgatcatgc
     1021 ctgtcatgcc gagccggcga atcgggcctg tacgctgcgc caaggcgacc caggtgatca
     1081 tgccgatgcc ggcgatcagg accgattgag gaacgtacaa ctggatgggt gcccgctcac
     1141 ccgtgatcgc ggcaacggcc acgatgcttg cgatcacgct ggtccagatc gccaagacgt
     1201 caaggcggcg ccagcatagg ggcgcgccaa gaaaggccac aagcagagga aatgtggggc
     1261 ccgcgaatgt gttactgagc agggccgaga ccgtagccgc cgtcaggagt atgccgctgt
     1321 cgcgctcgct catcgcggcc tggaccttct gatagtccag aaaattggat aaatccagcc
     1381 acaggccaac gttcgcaggc acccataccg cgagcccgac cccgaaggcg acgagcgcat
     1441 cgcgcatcag gcgcggccac gtctggccgg attgtctcgc catcacgagt accgccaggc
     1501 aaaacggcgc gaaaatgaag agcgccgagt gcttgaaact tatcgcgaga gcgaatgcga
     1561 tccccatcca tgcggcgtcg gcccggcgca gttcctgcgc ataccgcagc atggcccaaa
     1621 ttgccagcaa cgcgcttgct gccacaccca gatccggttt tccaatatgc gagcgccata
     1681 cactcaaggg cagcagcgcc attgccacac cgacggccag gcaaccggcc cgtgacaggg
     1741 cgagtcgacc cgcaatcatc gctgacagag gtgctgcgag ggctccagtg accgaaacca
     1801 caagccgcag gacgagcaga aaaatctccg gagtttcgaa gtacgcgtcg cggaacccgg
     1861 tcaggttcga ccacagaccc gccggtaatc cgatcaggaa cagaagcacg tctcccgccg
     1921 cggtgaggta attcaggagc ggcgggtaga agggctgcag ggggacaagt tcgccggtca
     1981 gcgccctcgc cgcatcgcca atattctcgt ccgggtgcct tccgccccac tccaagccga
     2041 cgcaccgcag tccaagtgcc aggacgaaaa gaccggccag ataccaccgc ccgatatggg
     2101 tcgctcctga tttttcctgg ggatcgttgc ggtgtaagga ttcgtccaac ttcatgaaac
     2161 ctatataccc cgttttgcgt gaatcttcag atcgtcgttt gtccgcggat gcaatcggga
     2221 tggcgattac gtccgcgacg tggattgggt cttggtaata ttctccgcag cgcgaggctg
     2281 atagggtcgg cccatcgttc tagcggccgg gcaaaggtgt cgcaattgcg aatgttacgc
     2341 gtgaattttc tggggtaatc ggtcgatcat gaagcttctt gcgcaagcgc tacgccttca
     2401 ccagtgggcc aagaacatgc tcctggcggt gcccctgctt accgcgcatc tggtcctgga
     2461 tctggaggcg tggaccagcg tcattgtcgc attcttcggc ttcggcttcg tcgcgtccgc
     2521 cacgtacctg gtcaatgatc tgatcgacct gaagtctgac aggcttcacg cgcagaagcg
     2581 gtttcgtccc atggcaagcg ggcgcttgac tgtatctgcg ggaatggcat tggcgatcgt
     2641 gctcgcattt ctcggatttg ctatttccgc ggcggccttg ccggccggat tcatccac
//
LOCUS       DATMZQ010000164         4271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1858250, whole genome shotgun sequence.
ACCESSION   DATMZQ010000164 DATMZQ010000000
VERSION     DATMZQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858250"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VN496_05395"
     CDS             <1..991
                     /locus_tag="VN496_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXF16415.1"
                     /translation="LLTHPPQVREHYYCDEALAALQALSEVRRNPLGRELSTAELIDA
                     AGNCEIIVTHRHVPGDRKLFASLPSLVAYCRCAVDIRNVDVEAASSQGVLVTHASAGF
                     IPATAEWVLGAMITLSRGIARCSSEYHAGRVPLPTMGRQLNGATLGVIGYGQIGRYLC
                     ELALALRMRVLVSDPYAQITSAGITQTELARLLSESDFVVCLAIANDETENLMNASAF
                     AKMKPGAFFINASRGNLVDEPALLRALDENRVAGCAMDVGRAADQMPSPALARHPNVI
                     ATPHLGGLTPQATLHQALETVAQVAEILKGHAPKGAVNADRASRLARFRKNAT"
     gene            993..2024
                     /locus_tag="VN496_05400"
     CDS             993..2024
                     /locus_tag="VN496_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXF16416.1"
                     /translation="MSHQRIAIIGLGMAVAPHAKSLIDLAARVQVACAYSPSAARRKS
                     FGTNFPFPQCDSFDAILNDASIDAVAVLTPPNTHLDIVSRCAAAGKHVLLEKPVEINT
                     ARAQQLVAVCRAAGVTLGIVLQHRHKPAVQRLAELVREGQLGAAVGASASIRLWRAQS
                     YYDEPGRGTRARDGGGVLMTQAIHTLDVLLSLAGDPAQVMCFATTSPVHKMESEDLVC
                     AGVRWRTGAYGVIEATTAAYPGAAERIDLIGSRATATLLGTGLEVRWQDGRSESLAAD
                     GSTGGTGADPMAFPHDYHRSVWQDFLDAIATGREPRVNGEEALRVHRLIDALLHSARG
                     AAPIDCIGA"
     gene            2041..3360
                     /locus_tag="VN496_05405"
     CDS             2041..3360
                     /locus_tag="VN496_05405"
                     /inference="COORDINATES: protein motif:HMM:NF027805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXF16417.1"
                     /translation="MDQKHSLMILNPGHFHAALTLRERHPLIDDDVHVFAEDGPDVDQ
                     FVHLVHAFNNRPVDPTRWTLYVYRGADFLEKMLSRRPGEMVVVAGRNNKKLECIHLLH
                     GQGLHVLGDKPWLIDSAQLELLKGVTATAPLAMDIMTERHELTNRLQKAFASQPSVFG
                     DFRIERDKPAIAIRSVHHLHKTVNRQPLRRPAWYFDTEQQGEGVTDVTTHLVDLAQWM
                     TGDTVPFRFERDVKALSACQWGTAVPRELFSCITGLDDFPAFLHDRVRNGQLQYLCNA
                     ALSYELRGLPVEIETLWDLDEPPGGGDLHHAILRGTRSDLIVDQGPGTQFVPTLSIQP
                     VERSKGYAEALGDAVDQMRNQFPGLGVEAAGTGFRVTIPPALRTTHEQHFAVVLQDFL
                     TYIDEGRWPASLGPDLETKYSLLAQALDLCHRGAPVAPTRTSSNTQL"
     gene            3386..4132
                     /locus_tag="VN496_05410"
     CDS             3386..4132
                     /locus_tag="VN496_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16418.1"
                     /translation="MGKIMIVTGSGRGIGAATALLAAQRGYAVCVNYLHNRNAAERVV
                     RDIEKTGGQAIAVGADVASEAAVMRLFETVDKEMGRVSALVNNAGILEKQTRLDGIDA
                     ARLTRVLATNVIGAFMCSREAVRRMSTKHGGQGGGIVNVSSRASQIGSPGEYIDYAAS
                     KGAVDSLTLGLAKEVAEEGIRVNAVRPGFIYTEMHASGGEPERVERVKAFAPMKRGGK
                     AEEVARAILWLLSEEASYTTGAFIDVSGGR"
BASE COUNT          887 a         1237 c         1355 g          792 t
ORIGIN      
        1 cctcctcacg catccgcctc aagtccggga gcattactac tgtgacgagg ccctcgctgc
       61 attgcaggcg ctgagcgagg tgcgccgcaa tccgttgggg cgcgagttga gcaccgcgga
      121 attaatcgac gcggctggta actgcgagat tatcgtgacg catcgccatg tacccggcga
      181 taggaaactg ttcgccagcc tgccctcgct agtggcgtat tgccgttgcg ccgtggatat
      241 tcgcaacgta gatgttgaag cggcatcatc gcaaggcgtg ctggtgacgc acgccagcgc
      301 aggtttcatt ccggccactg ccgagtgggt gcttggagca atgatcacgc tgagccgcgg
      361 catcgcccgg tgcagctccg aataccacgc gggcagggta ccgctgccga cgatgggccg
      421 gcagctgaac ggggcgaccc ttggagtgat cggatatggc cagatcggcc gctatttgtg
      481 cgaactggcg ctggcacttc gcatgcgcgt cctggtctcc gacccatacg cgcagattac
      541 cagtgccggg attacccaga cggagcttgc gcgcttgctg tcagaatccg attttgtcgt
      601 ctgcctcgca attgccaacg atgaaaccga aaacctgatg aatgcgtccg cctttgccaa
      661 gatgaaaccg ggtgcttttt ttatcaatgc atcgcgcggc aatctggtgg atgaaccggc
      721 actgctgcgc gcgctggatg aaaatcgtgt cgccggctgc gcgatggacg tcggccgtgc
      781 tgcggatcag atgccttcgc ctgcgcttgc acgccatcca aatgtcatcg ccacgccgca
      841 tctcggcggg cttacgccgc aagcaacgct gcatcaggca ctcgagacag tcgcgcaggt
      901 tgccgagata ttgaaggggc acgcacccaa gggtgcagtc aacgccgacc gggcaagccg
      961 gctggcacgg ttcagaaaaa atgcgacgta acatgagcca ccaacgcatt gccatcatcg
     1021 ggctcggcat ggcggtggca ccgcacgcaa aaagtctcat cgatcttgcg gcgcgcgtgc
     1081 aggttgcctg cgcctatagt ccgagcgcag cgcgaagaaa gagcttcggc acaaacttcc
     1141 cgttcccgca atgcgacagc ttcgatgcca ttttgaatga tgcaagcatc gatgccgtcg
     1201 cagtgctgac tccgccgaac acgcacctgg acattgtcag ccgctgcgcg gctgcgggaa
     1261 aacatgtact gctggaaaag cccgtggaaa ttaacacggc acgggcgcag cagctggtcg
     1321 cggtctgtcg cgcggcgggt gttacgctcg gcatcgtgct tcagcatcgg cataagcccg
     1381 ccgtgcagcg gcttgctgaa ttggtacgcg agggtcagct cggcgcagcg gtcggtgcat
     1441 cggccagcat tcggttatgg cgcgcacaga gttattacga cgaaccgggg cgcggtacac
     1501 gcgcgcgcga cggtggcggc gtactgatga cccaggcgat ccataccctg gatgtcttgc
     1561 tgtccctggc cggtgatccc gcgcaggtca tgtgctttgc aacgaccagc ccggtacaca
     1621 agatggaaag cgaagacctg gtctgtgctg gcgtgcgctg gcgcaccggc gcatacgggg
     1681 tcatcgaagc aaccaccgca gcctatccag gagccgcgga gcgcattgat ttgatcggct
     1741 cccgcgcgac agcgacgctc ctgggaaccg gactggaagt cagatggcag gatggccgca
     1801 gcgaatcgct tgcggccgat ggttccactg gagggaccgg cgccgacccg atggcttttc
     1861 cccacgacta tcatcgcagc gtatggcagg attttctgga tgccattgcg accgggcgcg
     1921 agccgcgcgt taacggggag gaagcactgc gggtgcatcg gttgattgat gcgctgttgc
     1981 atagcgcgcg cggcgccgcg ccgatcgact gcattggcgc ctaaacggga cgacagtcgc
     2041 atggaccaaa aacatagcct gatgatcctc aatcccggcc atttccacgc ggcgctcacg
     2101 ttacgggaaa ggcatccgtt gatcgacgac gacgtccatg tctttgccga ggacggcccg
     2161 gacgtggatc agttcgtgca ccttgtccac gccttcaata accggccggt ggacccgacg
     2221 cgctggacgc tctacgtcta tcgcggtgcg gattttctgg agaaaatgct ctcgcgcagg
     2281 cccggtgaaa tggtcgtggt tgcgggcaga aacaacaaaa aattggaatg cattcatctg
     2341 ttgcacgggc aaggtctgca cgtgctgggc gataagccat ggctcatcga tagcgcgcag
     2401 ctcgagttgc ttaaaggagt tacggcaact gcgccactgg ccatggatat catgaccgag
     2461 cgtcatgaac tgaccaatcg gctgcagaaa gcgtttgcga gccagccgtc ggtattcggg
     2521 gattttcgca tcgaacgcga taaaccggcc atcgccattc gcagcgtcca tcatttgcac
     2581 aaaaccgtaa acagacaacc gctgcggcgc ccggcgtggt acttcgatac cgagcaacag
     2641 ggggaaggcg ttaccgatgt cacaacgcac ctggttgatc tggcgcagtg gatgactgga
     2701 gacaccgtgc cgttccggtt cgaacgagac gtgaaggcgc tatcggcatg ccaatggggg
     2761 accgcagttc cgcgcgaact gttctcgtgc attaccgggc tggacgattt tccggcattc
     2821 ctgcatgacc gcgtaagaaa cgggcaactg cagtaccttt gcaatgcggc actatcgtat
     2881 gaactgcgcg gcctgccagt cgaaatagaa acgctgtggg atctggatga gccgccgggc
     2941 ggtggtgacc tgcatcatgc catcctgcgc ggcacgcgat ccgatctgat cgtcgatcag
     3001 ggccccggaa cgcaatttgt gccgacgcta agcatccagc ctgttgagcg cagcaaggga
     3061 tatgccgaag cgctcggtga cgcggtcgac cagatgcgga accagtttcc cgggctcggc
     3121 gtggaggcgg ccggaaccgg tttccgcgtc acaatcccgc cagcattgcg taccacgcac
     3181 gagcagcatt ttgcagtggt gctgcaagat ttcctgactt atatcgatga aggccggtgg
     3241 ccggcgagtc tcggaccgga cctcgaaacg aaatattcgc tgctggcgca ggcgctggac
     3301 ctgtgccatc gcggagcgcc ggtggcgcca acacgcacca gttcaaatac acagctttga
     3361 ccatggaagc cgacgacaaa cgccgatggg caaaataatg attgtgaccg ggagcggacg
     3421 gggcataggc gcggcgaccg cgctgctggc cgcccagcgc ggctatgcgg tatgcgtgaa
     3481 ttacctgcat aaccgcaacg ccgcagagcg cgtcgtgcga gacatcgaaa aaaccggcgg
     3541 acaggctatc gccgttggcg cagacgtggc ttccgaagcc gctgtcatgc ggctgttcga
     3601 gactgtagac aaggaaatgg gccgcgtcag cgcgctggtg aacaatgccg gcattctgga
     3661 gaagcaaacc cggctggacg gaatcgacgc cgcgcggctg actcgcgtct tggcgaccaa
     3721 tgtcattggg gctttcatgt gttcgcgcga agccgtgcgg cgcatgtcca cgaagcacgg
     3781 cgggcagggg ggcggaatcg tgaatgtttc atcgcgcgca tcgcagatcg gctcgccggg
     3841 cgagtacatc gactacgcgg cgtccaaggg cgccgttgat tcactcaccc tgggtctcgc
     3901 caaagaggtg gcggaagagg gtatccgggt gaacgccgtg cggccgggat ttatctatac
     3961 cgaaatgcat gccagcggcg gcgagccgga gcgggtcgag cgcgtcaagg ctttcgcacc
     4021 gatgaagcgc ggtggcaagg cggaagaagt cgccagggcc atcctgtggc tgctgtcaga
     4081 ggaggcgtcc tacaccacag gggcctttat cgatgtgtcc ggagggcgct aaatagctcc
     4141 aacggggggt tcaaaaagga cgcaaatagc tcaaagaaaa gacgataatt gagttcgcca
     4201 aggtaatcag tattcatttt gactatccag acttagagag gtgacagatt ggccaaaccc
     4261 aactactcat t
//
LOCUS       DATMZQ010000165         3652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2548671, whole genome shotgun sequence.
ACCESSION   DATMZQ010000165 DATMZQ010000000
VERSION     DATMZQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548671"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VN496_05415"
     CDS             complement(<1..1461)
                     /locus_tag="VN496_05415"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HXF16419.1"
                     /translation="MDFSNHAILIGAVLVLLSILASNVAQRTGAPLLFVFLAIGMLAG
                     EDGPGHIRFGDYQAAYLVGTMALAVILFDGGMRTRYETFRVGLWPAVSLASFGVVLTT
                     VIVGYGASRILGLGLMEGMLIGAIIGSTDAAAVFSLLHAAGMRLKQRVGATLEIESGC
                     NDPMAVFLTLSLVQIVGADQHAPGWSLLVHFIQQFGLGALAGIGGGRLLVLLIRRVTL
                     ASGLYPLLAMAGGLAIYAITTIFDGSGFLAIYLAGLVLGNSRVHTVGSILKVHDGLAW
                     LSQITLFVILGLLVTPSALFEIGLGALGVAALLMFVARPIAVTLSLLPFRFPLREQIF
                     ISWTGLRGAVPIVLALFPILGGLPQSSIYFNVAFFVVLASLLVQGWTVAPMAGWLKLK
                     LPKEHAPYYSEFVDVPGRAGLQLLGYRVNDLSAAIGRTVIQLALPSDVRVVAAFREGK
                     PVDPIGHLEIAAGDLVYVITPSEHMTVLDLLFVPADD"
     gene            complement(1600..2373)
                     /locus_tag="VN496_05420"
     CDS             complement(1600..2373)
                     /locus_tag="VN496_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF16420.1"
                     /translation="MRRGLLIVCQIGLLAGVFALWHVLTATETLPKFFFGEPLVVLAR
                     VRDWFVSGKIYPHLGVTLIETLLAFIIGTALGLVFGLWLALSDFASRLLNPYITALNS
                     MPRVILAPIFAVWFGLDIKSKVMLGITLVFFIVFFSVYNGVREVSPTILANARMLGAN
                     GRQLLRHVYLPSATAWVFSSLHASVGMAFVGAVVGEYLGSARGVGYLILQAEGVFDIN
                     TVFAGVLVLTAAALVLDYAVTLTERRLLRWRPGRVSAVS"
     gene            complement(2551..3351)
                     /locus_tag="VN496_05425"
     CDS             complement(2551..3351)
                     /locus_tag="VN496_05425"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF16421.1"
                     /translation="MNTSLQLDGVSCTFAPITGNAGYTAVDDVSLAIAQGEFVSIVGP
                     TGCGKSTLLNIAAGLIPPTRGSVRVFGEKLRGLNTRAGYLFQSEALMPWRTALQNITA
                     GLEFRHVPARQSQRRAQDWLRRVGLGAHGHKYPHELSGGMRKRVALAQTLILDPNILL
                     MDEPFSALDVQTRSLMENELLNLWAQDRKSVAFVTHDLEEAISLSDRVLVMSAGPASR
                     MIGEFAIDLPRPRDVSEIRLTQRFVELHKLIWQALRDEVQKAYSLSLS"
     gene            complement(3362..>3652)
                     /locus_tag="VN496_05430"
     CDS             complement(3362..>3652)
                     /locus_tag="VN496_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF16422.1"
                     /translation="TNAIVRALLWLQHHTTEDVLNAVPPEYLLGDRALYAASFERLRA
                     TYSKDGMIPKKGVDNSYKVLLAHNPAVRRAPVVWINQTYTNTFVEKALQQYR"
BASE COUNT          846 a         1131 c         1040 g          635 t
ORIGIN      
        1 atcgtcggcc gggacaaaca acagatccag cacagtcata tgctcgctcg gcgtgatcac
       61 atagactaaa tctccggcgg caatttcgag gtggccgatg ggatcgaccg gcttgccttc
      121 gcgaaaagcg gcaacaacgc gcacgtcgct cggcagtgcc agttgaatca cggtgcggcc
      181 gatcgccgca ctcagatcgt ttacacggta accgagcaat tgcaaccctg cccttcccgg
      241 cacgtcgaca aactccgaat aatacggtgc gtgctccttc ggtagtttga gcttcagcca
      301 tccggccatc ggcgcgaccg tccacccttg caccagcaat gaagccaaca ccacgaagaa
      361 cgcgacattg aagtaaatcg acgattgcgg caatccgccc agaatcggga acagcgccag
      421 cacgatcggc accgcaccgc gcaaaccggt ccaggaaata aaaatctgct cccgcagcgg
      481 aaaccggaac ggcagcagcg acagggtgac agcgattggc cgggcgacga acatgagcaa
      541 tgcggcaaca cccagcgcgc ccagtccgat ctcgaacagc gcgcttggag tcaccagcag
      601 gccgagaatg acgaacaggg taatctgcga aagccacgcc agcccgtcat gcaccttcag
      661 gatgctgcct acggtgtgca cgcggctgtt gccgagcaca aggccggcaa gatagattgc
      721 gagaaagccg ctgccatcga aaatggtcgt gatggcatag attgcaagcc cgcctgccat
      781 ggccagcagc ggatacagcc cgctcgccag cgtcactcgc cggatcagga gcacaagcag
      841 gcggccaccg ccaatgccgg ccagcgcccc gagtccgaat tgctggatga aatgcaccag
      901 caacgaccaa cccggagcgt gctgatccgc accgacgatc tgcaccagcg acaacgtcaa
      961 gaacaccgcc atcggatcgt tgcaaccgga ttctatttcg agcgtggcgc cgacgcgctg
     1021 cttaaggcgc atgcccgccg cgtgcagcag cgagaacacg gcagccgcgt cggtggagcc
     1081 aatgattgca ccgatcagca tgccttccat cagtcccagg ccgaggatgc gcgaggcacc
     1141 gtaacccacg atcaccgtcg tcaataccac gccgaaggaa gcaagcgaca ccgcgggcca
     1201 caacccgacg cggaacgtct cgtaacgtgt ccgcatccca ccgtcgaaca ggatgaccgc
     1261 aagtgccatc gtgcccacca gataagcggc ctggtaatcg ccgaaacgga tatgtccggg
     1321 cccgtcttct ccggccagca tgccaatcgc caggaatacg aacagcagcg gcgcgccagt
     1381 gcgctgtgcg acgttgctgg ccaggatgct caacagcacc aatacggcgc caatcagaat
     1441 tgcgtggttg gaaaaatcca agtgcgtttt tcaggtgaat gagccaaacc tgtttctagc
     1501 acggagtgga cgtttcgctc aagacaatcg agcaattgaa ccacgcgtta caagatcgat
     1561 cgcgccggga caattcggac taaccaacag atcagcaggt caggacaccg ccgacaccct
     1621 tcccggccgc caacgcagta gccgcctctc cgtcagtgta acggcgtagt caagcacgag
     1681 cgcggctgcc gtcagcacca gcactcctgc aaaaacggtg ttgatatcga atacaccctc
     1741 ggcctgcagg atcagatagc ccacgccgcg cgccgagcca agatactcgc cgaccacggc
     1801 accaacgaaa gccatgccca cggaggcatg caagctggaa aatacccagg ccgtcgcgct
     1861 gggcaagtaa acatgtcgca ataactggcg gccgttggcg ccgagcatgc gtgcgttcgc
     1921 cagaatcgtc gggctgacct cgcgcacacc gttgtagacg ctgaagaaca cgatgaaaaa
     1981 aaccagcgtg atgccgagca tgactttgga tttgatatcg agcccgaacc agaccgcgaa
     2041 gatcggcgcc aatatgacgc gcggcatgga attcagcgcg gtgatatagg gattgagcaa
     2101 ccgcgacgcg aagtcggaca gtgccagcca gaggccgaac accagcccaa gcgccgtgcc
     2161 gatgatgaac gcgagcagcg tttctatcag ggtcaccccc aaatgcgggt aaatcttgcc
     2221 gctgacgaac cagtcgcgca cgcgcgcaag gaccaccagc ggttcgccaa agaaaaactt
     2281 cggcaaggtt tccgtcgcgg tgagtacatg ccacagcgcg aacacgccgg ccagtagccc
     2341 gatctggcag acgatcagca ggccgcgcct catatcagtt ctgaaccacg gaggagacgg
     2401 agatcacgga gaaaaagaga ggaagccgcc aagtgaaaat ttgagtgctg acatcttcct
     2461 gacgccagca ttgccgcgat tccacggtag gccgcatttc tttcgttcat ttctccgtgt
     2521 cctccgtgtt ctccgtggtt cacgatttag tcaagaaagc gacagcgaat acgccttttg
     2581 cacttcgtcg cgtagcgctt gccagatcag tttgtgcagc tcgacaaagc gctgggtcag
     2641 cctgatttcc gatacatccc gcgggcgcgg caaatcgatc gcgaattcac caatcatccg
     2701 gctggccggt cctgcagaca tcacgaggac ccggtcggac agcgaaatgg cttcttcgag
     2761 gtcatgcgtg acgaatgcca ccgacttgcg atcctgtgcc cacagattca gcagttcgtt
     2821 ctccatgaga ctgcgggtct gcacgtccag cgcagagaac ggttcgtcca tgagcaggat
     2881 gttgggatcc agaatcagcg tttgtgccag cgccacgcgt tttctcatgc cgccggaaag
     2941 ttcatgcgga tacttgtgtc catgcgcgcc gagccccacg cgcctcagcc aatcctgtgc
     3001 gcgccgctgt gactgccggg cagggacgtg acgaaattcg agccccgcgg ttatgttttg
     3061 caacgcggtg cgccagggca tcagcgcttc gctttgaaac aggtagccgg cgcgcgtatt
     3121 gaggcctcta agcttttcgc cgaacacgcg cacgctgcct cgcgttggcg ggatcaagcc
     3181 ggcagcgata ttcagcagcg tggacttgcc gcatccggtc gggccgacga tcgatacgaa
     3241 ttctccctgc gcaatggcga ggctcacgtc atcgaccgcc gtatacccgg cgttgccggt
     3301 tatgggcgca aacgtgcagc tcacgccgtc cagttgcagc gatgtgttca tgtccggccg
     3361 cttatctgta ttgctgcagc gctttttcga cgaaggtgtt ggtgtaggtt tgattgatcc
     3421 agactacagg cgcacgccgt actgcggggt tatgcgccaa cagcacctta taggaattgt
     3481 cgacgccttt ctttggaatc atgccgtctt tcgaatacgt tgcgcgcagc cgctcgaatg
     3541 atgcggcata gagcgccctg tcgccaagca aatattccgg cggcacggca ttcaaaacgt
     3601 cttcggtggt atggtgctgc agccagagca acgcgcgcac aatggcattg gt
//
LOCUS       DATMZQ010000166         6818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1450239, whole genome shotgun sequence.
ACCESSION   DATMZQ010000166 DATMZQ010000000
VERSION     DATMZQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450239"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3245)
                     /locus_tag="VN496_05435"
     CDS             complement(<1..3245)
                     /locus_tag="VN496_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16423.1"
                     /translation="MASRTTISLLAQLLRSGLRWATPSESAYRATSFTFLTWLVALEA
                     YKLANKNDRQNTVADDTAAKSHDEDTGSASSRLDEAQDARDAEVHPEQLAKLDDTTTD
                     KLRAEADLDDYLADVIGSSTDPRILAAAIPPSFKAPVTVGGVSPVLGAGIRADSGVTP
                     AANHANFEPVQVVTATAPQPGATVPSVSSVAASGSGIDAGGNGDLNAGHVVTLTLNMS
                     GVVTVGGGVPTLSLNNGATATYTGGSGSNVLTFSYTVGAGGDTSDLAVTSFNLNGAKV
                     RDAAGNNADVVGAVANPPGILQIDTTAPTVPSVAASGSGIDAGGNGDLNAGHIVTLML
                     TMSETVTVAGGVPTLSLNSGGTASYTGGSGSNTLTFSYTVGAGEGTSDLAVTSFNLNG
                     AIVSDPAGNSANLLGAMTNPSGVLQIDTTAPTVSSIAISGSGIDAGGSGDLNTGHVVT
                     LTLTMSEVITVAGGVPTLSLNSGGTASYTGGSGSNTLTFSYTVGAGEDSSDLAVTSFN
                     LNGAMVGDAAGNNANIAGAITNPPGVLQIDTTAPTVPSVAATGSGIDAGGNGDLNAGH
                     VVTLTLSTSEVVTVAGGVPTLSLNSGGTASYTGGSGSNTLTFSYTVGAGEDTSDLAVT
                     SFNLNGATVSDAAGNSADLLGAATNPVGILQIDTAAPAAPAIVNIALGGVGGNHWVLT
                     GTAEANSTVAIFDGNIQLATATASGAGAWTYTTTGAVTNSAVHTFTATASDPAGNTSA
                     VSAAWVVGSSGNDIFTFGSQVQLTAPIAISGNGGADIIALTAPVTLNSGDFVNVSGVQ
                     SLQLTGANSVTFGGDAVTAGIVNVVTGNSATSIADSNSVPLNVDATSLADNTGLSLSG
                     SAGFTVTGLQGDLTATGVSGALNVTTVAVASGLSIATGSGTNTLTASALTSGQTLTLT
                     GGSAASVTLNAGASTGNLAAGTYAGNLIVTGGSGANTITVGNGANTITGGGGADGLIG
                     GTGSDTFNFSSAVNLGAAATIAGGAGSDTIAVTAAATLTDANFLNATSIETLRLTGAS
                     TITLGANAANAGLVNVTTGNGATSITDSNGVTLNVDTTALANNTVLTLTGSS"
     gene            complement(4005..4892)
                     /locus_tag="VN496_05440"
     CDS             complement(4005..4892)
                     /locus_tag="VN496_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16424.1"
                     /translation="MALFSKRKLSPIERFESALKDKHVAREKLTGRLRAAETALGEIR
                     AAATRLAMAAAADAQLDWAEADMRAAEDRTKTLRTALAQLDEQIAATERELATAKTQR
                     DRELVANELQRMATAIEQAAPNYDTAVVALVEIITKSEASIPETTRFATNLDAVRREV
                     LSAAEFICTELRSAAARTRAGNANFSVRANPGPEPAPPREIERQLVYTLNPLLWLEGG
                     EVRTVPAFAQVELPKMLLPVALRYQHADYLDARRVRTLMQVHGSGQFYCTLQHDDSRV
                     IDLDALAAQEMRDNGANAA"
     gene            5062..5328
                     /locus_tag="VN496_05445"
     CDS             5062..5328
                     /locus_tag="VN496_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16425.1"
                     /translation="MSQEEKQTVPVSVIELRAESYSVDGDNIVVSLRTKYSTAERKYS
                     VPIQCFQDLIVDLQRLNASTPIEPNEAHKQAEPFLPFEIPVAAE"
     gene            complement(5389..6144)
                     /locus_tag="VN496_05450"
     CDS             complement(5389..6144)
                     /locus_tag="VN496_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16426.1"
                     /translation="MKFIEPSFDPAGGAWPLPSNTHQKDIGDLENKIAKQSSELNLRL
                     TEVLELYTLQSRHANELDTAHEEISRLQRIISALHRIAKQQTIDAATTQDKITCLETD
                     KADLGAQLNQALQDSKSLADRLHAVQIVFDTRETNAASALEQVDFLNSELASSTAERF
                     NLVASMHGEKRRHNLQTAALEERLKKAESKVEKQEMQMKGLETVRCKLDKRIQVLEAL
                     LKSEHEIAERKIKRLTDELERNGRGISLPTPTK"
     gene            complement(6532..>6818)
                     /locus_tag="VN496_05455"
     CDS             complement(6532..>6818)
                     /locus_tag="VN496_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16427.1"
                     /translation="LSMLKLTSKFVLQILPYLLMAVAAVVLLPGVTDSLITATTRIVS
                     PPPPEIDTMIEPFGHDQTALDLIRQDHEVFAPRPSLHKIARAAEFDLLDR"
BASE COUNT         1367 a         1978 c         1950 g         1523 t
ORIGIN      
        1 gacgatcctg tcagcgtcag taccgtattg ttggccagag ccgtcgtatc gacgttgagc
       61 gtgacgccgt ttgaatccgt gatgctggtt gcgccgttcc ccgtggtcac attcaccagg
      121 ccggcattgg ctgcatttgc accgagcgta atggtgctcg cgccggtcag cctaagggtt
      181 tcgatgctgg ttgcattgag gaagttggca tcagtcagcg ttgctgccgc cgtcaccgca
      241 atggtgtcac tgcccgcccc gccagcgatg gttgcggcgg cgcccagatt gaccgccgaa
      301 ctgaagttga aggtgtcact gccggtgccg ccaatcagtc cgtccgcgcc gccgccgccg
      361 gtgattgtat tggcgccgtt tccgacagtg atggtattgg cgcccgaacc gcccgtgacg
      421 atgagattgc ccgcataagt gccggccgca aggttacccg tcgatgcacc ggcattgagc
      481 gtcacgctcg ccgcgctccc gccggtcagg gtcagcgtct gtccgctggt gagcgcgctc
      541 gcggtgagcg tgttcgtgcc gctgcctgtt gcaatcgaga ggcccgaggc gaccgcaacc
      601 gtcgtcacat tcagtgcccc gctaacgccc gtggccgtga gatcgccctg cagcccggtc
      661 accgtaaagc ccgccgaccc cgacaaactc aatccagtat tgtccgcaag tgaagtggca
      721 tcgacattga gcgggacgct attggagtct gcgatgctgg ttgcgctatt cccggtgact
      781 acattcacga tgccggcggt tactgcgtcc ccgccgaatg tgacactgtt tgcaccggtc
      841 agttgtaacg actgaacgcc gctgacattt acgaagtcgc cactgttcaa cgtaactggc
      901 gcggtcagcg ctatgatatc ggcgccacca ttgccgctga tcgcgatggg ggcagtcagc
      961 tgcacctgcg atccaaacgt gaagatgtcg tttcccgacg accccacgac ccatgctgcc
     1021 gagacagcgc tggtgtttcc agcaggatca gacgcggttg ccgtaaaggt gtggacggcg
     1081 gaattggtta cagcgccagt ggtcgtatag gtccacgcac cagcgcctga cgccgttgcg
     1141 gttgcaagtt ggatgttgcc gtcgaatatg gcgacggtgc tgtttgcctc cgcggttcct
     1201 gtgaggaccc aatgattgcc gccgacgcca cccaacgcga tattcacaat ggccggggcc
     1261 gctggcgccg ctgtatcgat ctgcaggata ccgaccgggt ttgttgcggc gcccaacagg
     1321 tcagcgctgt tgcccgccgc atcgctgacc gttgcgccgt tcaagttgaa ggacgtcacc
     1381 gcaaggtcgc tggtgtcctc tccggcgcca accgtgtaac tgaaggtcag agtattgctg
     1441 ccagagccgc ccgtgtagct tgcggtgcca ccactgttga gcgacagcgt tggtactcca
     1501 cccgcaaccg tgaccacctc gctcgtgctg agcgtcagcg tgaccacgtg gccggcattg
     1561 aggtcaccat tgccaccggc atcgatcccg ctgccggttg ccgcaaccga tggcaccgtc
     1621 ggggccgtcg tatcgatctg cagaacaccg ggcgggttcg ttatcgcgcc cgcaatattg
     1681 gcgttgttgc ctgcggcatc gccgaccatt gctccgttca agttgaagga cgtcaccgca
     1741 aggtcactgc tgtcctctcc ggcgccaacc gtgtagctga aggtcagagt gttgctgccg
     1801 gagccgcccg tgtagcttgc agtgccacca ctgttgagcg acagcgttgg tacgccgccc
     1861 gcaaccgtga tcacctcgct catggtgagc gtcagcgtga ccacgtggcc ggtattgaga
     1921 tcaccgctgc cacctgcatc gatcccgctg ccagagatcg caatcgatga caccgtcggt
     1981 gccgtcgtat cgatctgcag gactccggat gggttggtca ttgcgcccag tagattggcg
     2041 ctgttgcccg ccggatcgct gactattgca ccgtttaagt tgaaggacgt caccgcaagg
     2101 tcgctggtgc cctctccggc gccaaccgtg tagctgaagg tcagagtgtt gctgccggag
     2161 ccgcccgtgt agctggcagt gccaccactg ttgagcgaca gcgttggtac gccgcctgca
     2221 accgtcaccg tctcgctcat ggtgagcatc agcgtcacaa tatgaccggc attgagatca
     2281 ccattgccac cggcatcgat cccgctgcca gaggcagcaa ccgatggcac cgtcggtgcc
     2341 gtcgtatcga tctgtaggat gccgggcggg ttcgccaccg cacccacaac atcggcgttg
     2401 ttgcccgcgg cgtcgcggac ctttgccccg ttcaagttga aggacgtcac agcaaggtcg
     2461 ctggtgtccc ctccggcgcc aaccgtatag ctgaaggtca gcacattgct gccggagccg
     2521 ccggtatacg ttgcggttgc gccattgttc agcgacagcg tcggtacgcc gccgccaaca
     2581 gtcaccaccc cgctcatatt gagggtcagc gtgaccacgt ggccggcatt gagatcaccg
     2641 ttgccgcctg catcgattcc actgcctgat gccgcaaccg atgacaccga cggaaccgtc
     2701 gcaccgggct gcggcgcagt cgctgtgacg acctggaccg gttcaaaatt ggcatggttc
     2761 gcggctgggg tgacgccgct atcggcgcgg atgcctgccc ccaaaacggg agaaacgcca
     2821 ccaaccgtca caggcgcttt gaagctgggg gggatagctg ctgcaagaat tcgcggatcc
     2881 gtggaggatc cgatcacatc ggccaaataa tcatcaaggt cggcttcagc acgcagcttg
     2941 tcggtcgtcg tatcatcgag cttggcgagc tgttctggat gcacttctgc atcgcgggca
     3001 tcctgtgcct cgtctaatcg cgacgacgca ctccccgtgt cttcatcatg gcttttagca
     3061 gcggtgtcat ccgcaactgt attttggcgg tcgtttttgt ttgcaagctt gtacgcctca
     3121 agtgcaacga gccacgtcag aaaagtaaag ctggttgcac ggtatgcgct ttcagatggc
     3181 gtcgcccaac ggagcccgct acgcaacagc tgcgcgagaa gcgatatggt ggtccgagaa
     3241 gccaaaaata atttccttcc ggcagatcaa agatcttttc atgatcagtg ctgccgaagg
     3301 cgcttccaat cgatttcttg attggtaagt cagtatgact ccgagaccaa aaaaatagat
     3361 taagttgaaa gcaaaacggg gggtccccaa aatgcaattc attaacctaa agatcgctgt
     3421 catcgaaaac tcgtgcagta ccaatggctt ttgccaatcg ttgaagtgac gttgcaatgt
     3481 cacgactgaa aaaattttag acaagtgtag cgtaatttct attccagatt gaactttctt
     3541 ttcaaactac cgcgtgcgtt ggcaacgcaa cgcgcggatg cacactctgg gctcggaagt
     3601 cggaacggcg cggcctacgc aggaagaaat ctgaaccctg cggtgagttg tgaagacggt
     3661 ctcaatattc gcgagtgagc atgtctgttc attgtggcga ttgcgccaca ccagggcgat
     3721 gccctttaaa cggacgtcga tcggcgccgg gttgactaaa gctgtccgga gccagttttc
     3781 gcctaaaagg aggcaacaaa caaagcctac gaatagattg cctcccgctc cgctcacgga
     3841 gccgcgatgc ggttgggtgc atattcgcac cgcgcacgac gggcatcggg taaaccgcca
     3901 tgtacagtgc aacacgtccg agcgatatct gcccttcggc gaagccgata ctttcagtag
     3961 gtcaggacaa aactaggggt ggctatgttc cggcatcccg actctcacgc ggcattggca
     4021 ccgttgtccc gcatctcctg cgcagcaagc gcatcaaggt cgataacccg cgaatcgtcg
     4081 tgctggagag tgcaatagaa ctgtccgctg ccatgaactt gcatcagggt acgcacacgc
     4141 cgtgcgtcca gatagtccgc atgctgatag cgcagcgcga ccggaagcaa catttttggc
     4201 agctcaacct gcgcgaacgc tgggaccgtg cgcacctcgc cgccctccag ccaaagcaac
     4261 gggttgagcg tgtaaaccaa ttggcgctca atctctcgag gtggcgctgg ctctggcccg
     4321 gggttcgcgc ggactgaaaa gttcgcgttg ccagcgcgcg tgcgtgccgc cgcggatcgg
     4381 agctccgtgc aaataaattc cgctgccgaa agaacctcgc gacgaaccgc atccaaatta
     4441 gttgcaaacc tcgtcgtctc tggaattgac gcctcgcttt tcgtaataat ctccaccagc
     4501 gcgaccacgg ctgtatcgta gtttggagcg gcttgctcta tggccgtagc catcctctga
     4561 agttcgttcg ccaccaattc acggtcgcgc tgcgttttgg cggtggccaa ttcacgttcg
     4621 gttgcggcga tttgctcgtc gagttgtgcc aatgcagtgc gcaatgtctt agtgcggtcc
     4681 tcagcggcgc gcatgtcggc ttcagcccaa tcgagctgcg catcggcggc agcagccatt
     4741 gcgagacgtg tggctgcagc acgtatttct ccaagcgccg tctcggcggc gcggagacga
     4801 ccggtcagct tctcccgcgc cacgtgcttg tccttgagcg cgctttcgaa gcgttcgatc
     4861 ggactgagtt tacgtttgct gaatagtgcc acggctcagt actcgcaaat cgcgccggca
     4921 taataacgta cgctggcccg attcgcagca gaaaccgacg ccacagcatc gcaattacat
     4981 ctagccgaca atgaaagtgt ctggtggaaa accatcaaca ttggtagatt gcctataatt
     5041 catgagttgg gtggtggtgc tgtgagccag gaagagaagc agaccgtacc tgtcagtgtg
     5101 atcgaactga gggcggagag ttactccgtg gacggcgaca atatcgttgt ctcattgcgg
     5161 acaaaatatt ccacggccga acgcaaatat tctgttccca ttcaatgttt tcaagacctg
     5221 atagtcgatc tgcaacggtt gaacgcctca acgcctattg aaccaaacga ggcccataaa
     5281 caggcggagc catttcttcc gtttgaaata cccgtggcag ctgaataaca ctccccgcac
     5341 gttcgcaaag atatagatgc cttgtgcgcg cgatgtgtga cccgagcttt atttcgtcgg
     5401 tgtcggcaac gaaattccgc gaccgttgcg ctcaagttcg tcggtcaatc gctttatttt
     5461 tcgttcagct atttcatgtt cgctcttaag cagggcctcg agaacctgaa ttcgcttgtc
     5521 cagcttacag cgaaccgttt cgaggccctt catttgcatc tcttgttttt caactttgga
     5581 ctcagccttt ttgagtcttt cttccaaggc cgcggtttgc aggttgtggc gtctcttttc
     5641 gccatgcatg cttgccacca ggttgaatct ttccgcggtg gaactcgcca gctctgaatt
     5701 gagaaagtcg acctgctcga gcgctgaagc ggcgttcgtt tcccgtgtat cgaacactat
     5761 ctgcacagcg tgcagccgat ctgccaacga ttttgaatct tgaagggctt gattgagttg
     5821 ggccccgaga tccgccttgt cggtttccag acaggtgatc ttgtcctgtg tggtagccgc
     5881 gtctattgtt tgttgttttg cgatcctgtg cagcgccgag ataatccgct gcaggcggct
     5941 gatttcttcg tgcgcagtgt caagctcgtt agcgtgccga ctctgaaggg tgtaaagttc
     6001 gagaacctcg gtaagtcgca aattcaattc cgaggattgc ttggcaattt tattttcgag
     6061 gtcgccaata tctttttgat gagtatttga cggaagcggc caggcccctc cagcagggtc
     6121 aaagctaggc tcgataaatt tcatagtcgg gcgtttcccg ttttggagcg ggatcgaacg
     6181 catttttgct ggtacgaaga aaacggcgaa ccaggcgaat caatcgccgc tcgtccgacc
     6241 gactttccga ggaacccctt aagcagccct taacaggaga gtcgatgaag cgtgtttgga
     6301 gtgtgtccga aaaggaccaa accaatgttc agcctgaccg accgtgcttg gcgcgccatt
     6361 tcggcccggg tccgcgcatg tagtgtcgtt cgggccgata tcgcctctcg tgtggttccg
     6421 gccaatataa gaaaattcgg gctgccagcc gtgcgcgcac ctgatcccac caaatcgttc
     6481 gacgagacaa acacgacatt ccgctttgtg aacgcatgcg cgatcccttt cctagcgatc
     6541 caggagatca aattcagccg ctctcgcgat tttgtgtaag gatggtcttg gcgcaaagac
     6601 ctcatggtcc tggcggatga gatcaagcgc agtctggtcg tgaccgaatg gctcaatcat
     6661 cgtgtcgatc tcggggggcg gaggcgaaac gatcctggtt gtggcggtta tcagtgaatc
     6721 tgtaacccct ggcagcagaa cgaccgcagc caccgccata agcaggtacg gcaatatttg
     6781 caaaacaaac ttcgatgtca gcttaagcat ggaaaggc
//
LOCUS       DATMZQ010000167         4334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3240895, whole genome shotgun sequence.
ACCESSION   DATMZQ010000167 DATMZQ010000000
VERSION     DATMZQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240895"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..475)
                     /locus_tag="VN496_05460"
     CDS             complement(74..475)
                     /locus_tag="VN496_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXF16428.1"
                     /translation="MLKGSCLCKAVRYEIDGQLDNARHCHCSICRKAHGAAFRSRASV
                     RSGDFRWLAGADLITWYESSPGTQRGFCSRCGSALLSRFEADPSIYGLPLGCLDTDPG
                     VRPTMHIFVASKAPWFEITDTLPRYAEGPPR"
     gene            complement(492..1691)
                     /locus_tag="VN496_05465"
     CDS             complement(492..1691)
                     /locus_tag="VN496_05465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF16429.1"
                     /translation="MRNQSNKIILKAGREKSLLRRHPWVFSGAIARTERTGAAGDTVR
                     IVAQDGRALGIAAWNPDANISARIWDWDENTPIDASFFVNRLERAIRMRRELSARGPH
                     EAERLVHAESDGLPGLIVDRYADVIVLQISSAGCHRWRETIIDSLQQLTGARAIYERS
                     DADVLELEGLAPRTGLVKGRLDSPIVEVIENGMRLRVDVASGHKTGFYLDQRDNRLQV
                     GQLAADREVLNCFSYTGGFTLQTLAHGAARVTSVDSSAEALRLAREHISLNEMPQDRC
                     DWVDADVFEYLRRLRDHNRRFDLIILDPPRFAPTAASAERAARGYKDINLLAFKLLRP
                     GGLLATFSCSGGVSADLFRKIVAGAGLDAGCTVQILKQFHAAMDHPVLLTFPEGEYLK
                     GLLCRID"
     gene            complement(1706..2644)
                     /gene="xerC"
                     /locus_tag="VN496_05470"
     CDS             complement(1706..2644)
                     /gene="xerC"
                     /locus_tag="VN496_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXF16430.1"
                     /translation="MTGQETPSAAEQESAVDHALIRAFLSHLVHERRLSPHTAEAYGH
                     DVRVLLELAQEGALQDLQVHQIRRFIAQLHARGLGGKSLARMLSAWRGFFNYLVRDHG
                     FTQNPCIGLRPPRSSKSLPKALSPDEAVRLVTLPGDDPLAVRDRAMFELLYSSGLRLS
                     ELIALTQDDVSFADATVRVTGKGSKTRVVPVGKAALQALQAWLEIRQVGPSGDAGALF
                     VNPRGRAISPRVVQARLKAWGIKQGLAGHVHPHMLRHSFASHVLQSSGDLRAVQEMLG
                     HASISTTQVYTHLDFQYLAKVYDQAHPRAKRKPARR"
     gene            complement(2645..3289)
                     /locus_tag="VN496_05475"
     CDS             complement(2645..3289)
                     /locus_tag="VN496_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HXF16431.1"
                     /translation="MRPEDVARYLQDHPEFFETFSDLISSITIPHPHGGRAIPISERQ
                     IISLREKNRLLEAKLRELVEYGEENDAIGERVHQASLAFMAPASLETLLQILYYNLRE
                     DFAVPHVAIRLWSEWDHSPLPEFGKCSQEIRVFADSLKQPYCSHQPMFETTDWFGEGG
                     ALLKSFAYISLRTEKVFGLLALGSEDPKRFYADMGTLYLKRIGELISTALVRFL"
     gene            complement(3286..4128)
                     /gene="dapF"
                     /locus_tag="VN496_05480"
     CDS             complement(3286..4128)
                     /gene="dapF"
                     /locus_tag="VN496_05480"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXF16432.1"
                     /translation="MRLKFTKMHGAGNDFVVVDGTRNALRLDPAVIGKLADRHFGIGF
                     DQMLVVEPPRTPGTDFYYRIFNADGGEVSQCGNGARCFVRYVRDKGLSTKDAIRVETR
                     SGIIEPRMEKDGRVSVTMGVPVLEPSLIPFIAERQAPTYRLDVDGRNIKIFAVSMGNP
                     HAVQVVADVEAAPVLSEGPLIEHHPRFPQRTNAGYMQIVDRGHIKLRVFERGAGETLS
                     CGTGACAAVVAGISLGLLDSPVQVDTRGGMLQIGWGGLTEPVLMTGPAQSVFEGEIEV
                     EKTE"
     gene            complement(4119..>4334)
                     /locus_tag="VN496_05485"
     CDS             complement(4119..>4334)
                     /locus_tag="VN496_05485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF16433.1"
                     /translation="LIAHVAESARRGGMEELYLQVNKHNHASVAAYLRVGFVVVQAVK
                     ADIGNGFFMDDFVMSKPVVAAMNDACA"
BASE COUNT          912 a         1328 c         1238 g          856 t
ORIGIN      
        1 atatggccct ggatacgacc ttccgcattc cacgcggttt caccatggcg gaccatcacc
       61 agcctggtgt tgctcatcgc ggcggacctt ccgcataacg gggcagcgta tccgtaatct
      121 cgaaccaggg cgccttcgaa gcaacgaata tatgcatcgt gggcctcacg cccggatcgg
      181 tgtcaaggca accgagcggc agtccataaa tgctgggatc cgcctcgaag cggctcaaca
      241 gcgctgaacc gcaccggcta cagaaaccgc gctgcgtgcc aggtgaggac tcgtaccagg
      301 taatcaggtc ggccccggca agccaacgaa aatcaccgga acggacgctg gcgcgcgagc
      361 gaaatgccgc cccgtgcgcc ttgcgacaga tggaacaatg gcaatgacgc gcgttgtcga
      421 gctgtccgtc gatttcataa cgcacggcct tgcaaagaca acttcctttc aacatgttct
      481 ctcccgggta tttagtcgat acggcacagc aatcccttca ggtactcgcc ttccggaaac
      541 gtcagtagca cagggtggtc catcgctgca tgaaactgct ttaggatctg cacggtgcag
      601 cccgcatcca acccagcgcc tgcgacaatc ttgcggaaca aatcggcaga tacgccaccc
      661 gagcacgaga acgtggctag caggccgcct ggccgcaaca gcttgaatgc gagcaggttg
      721 atatccttgt agccacgcgc ggcacgctcg gcgcttgccg cggtcggagc aaaccggggt
      781 gggtcgagga taatcaaatc aaagcgccgg ttgtggtcac gcagccggcg caggtattcg
      841 aatacatcgg catcgaccca gtcacagcga tcctgcggca tctcattcag gctgatgtgc
      901 tcccgcgcca ggcgcagggc ctccgcggat gaatcgactg aagtgacccg agcggcgccg
      961 tgcgccagtg tctgcaacgt gaagccaccc gtatagctga agcaattgag cacttcgcgg
     1021 tctgcggcga gctggcccac ttgcaggcga ttgtcgcgct gatcaagata gaatccggtc
     1081 ttgtgtccgc tggccacgtc aacgcgcaaa cgcatgccgt tttcgatcac ctcgacaatc
     1141 ggggaatcca gcctgccttt caccagcccg gtgcgcggcg ccagcccttc gagctccagc
     1201 acatcagcat cggaacgttc gtagatggcg cgtgcaccgg tgagctgctg cagcgagtcg
     1261 atgatggtct cccgccatcg atggcagccc gcgcttgaaa tttgcaatac gattacatcc
     1321 gcatagcgat cgacgatcaa tcccggaagt ccatccgatt ctgcatgcac caggcgttcg
     1381 gcttcgtgcg ggccccgggc agacagttcc cggcgcatgc gaattgcgcg ctccagccga
     1441 ttgacgaaaa acgatgcgtc aatgggcgta ttttcgtccc agtcccatat gcgggcggaa
     1501 atatttgcat ccggattcca tgcagcgatg ccgagagcgc ggccgtcctg tgcaacgatc
     1561 cggaccgtat cgccggcggc accagtccgc tcagtgcgcg cgatcgctcc agagaaaacc
     1621 cacggatgtc ggcgcaacaa agatttctcc ctgccagcct tcagaatgat tttgttcgat
     1681 tgattccgca cgacgggtac agcggttacc gtctggcagg ttttcgtttg gcccgcggat
     1741 gtgcctgatc gtacactttg gcgaggtact ggaaatcgag atgcgtgtac acctgggtgg
     1801 tggaaatgct ggcgtggccc agcatttcct gtactgcacg caaatcgccg ctcgattgca
     1861 ggacatgcga agcaaatgaa tggcgcagca tatgcggatg cacgtgcccg gcaagtcctt
     1921 gcttgatgcc ccacgccttt aatcgagcct ggaccacgcg tgggctaatg gcccgcccac
     1981 gggggttcac aaacagcgcg ccagcatcgc cggatggccc tacttggcgt atctcgagcc
     2041 aggcctgcaa tgcctgcaac gcagccttgc caacaggaac cacgcgcgtc ttgctgccct
     2101 tgccggtcac ccgcaccgtg gcatcggcaa agctgacgtc gtcctgtgtt aatgcaatca
     2161 gttcggacag ccgcaaacca gacgaataca gcagttcaaa catcgcccgg tcacgcaccg
     2221 ccagcggatc gtcacccggc agagtgacca ggcggaccgc ctcgtcaggc gatagcgcct
     2281 tgggcagcga tttcgatgaa cgcgggggcc gcagtccaat acacggattt tgggtgaagc
     2341 catgatcgcg cacgaggtaa ttgaagaaac cacgccaggc tgaaagcatg cgcgccagcg
     2401 acttgccgcc cagcccgcga gcgtgcagtt gtgcgatgaa gcgccggatt tgatgaacct
     2461 gcaaatcctg caacgcaccc tcctgcgcga gttctagcaa cacacgaaca tcgtgcccgt
     2521 acgcttcggc ggtgtgtggg gacaggcgcc gctcatgaac caggtgcgac aagaatgcgc
     2581 gaatcaacgc gtgatccacc gcggattcct gctcggctgc ggatggcgtt tcttgacctg
     2641 tcatctacaa aaagcgcacc agcgccgtac tgatgagttc cccgatgcgt ttcaagtaca
     2701 gagtccccat gtcggcatag aagcgtttcg ggtcttcgct gcccagcgca agcaggccga
     2761 acaccttttc cgtgcgcagg ctgatgtagg caaaggattt gagcagggcg ccgccctctc
     2821 cgaaccagtc ggtcgtttcg aacattggct ggtggctgca gtatggttgc ttgagactgt
     2881 ccgcgaacac gcggatttcc tggctgcact tgccgaattc cggcaaaggc gagtgatccc
     2941 attcggacca caggcgtata gccacatggg gtacggcgaa atcctcgcgc aagttgtaat
     3001 agagtatttg cagtagcgtt tccaggctcg ccggggccat gaacgccagc gaggcttgat
     3061 gcacgcgttc tccaatggcg tcgttctcct cgccgtattc caccagttcg cgcagcttag
     3121 cctccagcaa ccggttcttt tcgcgcaggc tgatgatctg ccgttcagag atcggaatgg
     3181 cacggccgcc gtgcgggtgc gggatcgtga tgctgctgat caggtcggag aacgtctcga
     3241 aaaattctgg atggtcctgc aaataacggg cgacgtcttc aggtctcatt ctgttttctc
     3301 aacctctatt tcaccttcaa atacggattg tgccggaccg gtcatcaaga ccggctccgt
     3361 cagtccgccc caaccaattt gcagcatgcc accgcgcgtg tccacttgca ccggcgagtc
     3421 cagcagtcca agcgaaatcc cggcaacgac tgctgcacag gcgccggtgc cacaggaaag
     3481 cgtttccccg gcgccgcgct cgaacacgcg cagtttaatg tgcccgcgat cgacaatttg
     3541 catatatccg gcattggttc gttggggaaa gcgcggatgg tgctcgatta gcgggccttc
     3601 actcaatacc ggcgccgctt ccacgtcggc caccacttgt acggcatgcg ggtttcccat
     3661 cgatacggca aagattttga tgttgcgtcc atcgacatcg agccggtaag tgggtgcctg
     3721 gcgttctgcg atgaatggaa tcaacgaagg ttcaaggacc ggcacgccca tggtcacgct
     3781 cacgcggcca tccttctcca tgcgcggttc aatgataccg gagcgcgttt ccacgcggat
     3841 tgcgtctttg gtcgaaaggc ccttgtcacg cacatagcgt acgaagcagc gcgcgccatt
     3901 tccgcattgg ctgacttcac cgccgtcggc attaaaaatt ctgtagtaaa agtcggtgcc
     3961 cggcgtacgc ggcggctcga cgaccagcat ctggtcgaag ccgatgccga agtgacggtc
     4021 ggcaagcttg ccaatgactg cgggatccag acgcaatgcg ttgcgcgtgc catcgacaac
     4081 aacgaagtcg ttgccggcgc cgtgcatctt ggtgaacttc aagcgcatgc gtcgttcatt
     4141 gcggcaacta ccggcttgga catgacgaaa tcatccataa aaaaaccatt gccaatgtcg
     4201 gccttcacgg cctgtaccac gacgaaacca acacgcaaat aagccgctac cgacgcatga
     4261 ttgtgtttgt tgacctgcaa gtacagttct tccatgcccc cgcggcgagc tgactccgcc
     4321 acatgcgcga tcag
//
LOCUS       DATMZQ010000168         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3933258, whole genome shotgun sequence.
ACCESSION   DATMZQ010000168 DATMZQ010000000
VERSION     DATMZQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933258"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VN496_05490"
     CDS             complement(<1..819)
                     /locus_tag="VN496_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF16434.1"
                     /translation="MVDRTNTTRFRFWLWLIWLFGVIVPRSLRADWKQEWQAELQHRE
                     LLLADWDKLNWKTKLDLLCRSLGAFWDALWLQPQRLEDEMFQDLRYGARMLVKNPGLT
                     GVVLLTLALGIGANAALFSVVNGVLLNPLPFPEPERLITLHQSKPNFETGAIPYPNFR
                     DWQKENQTFSAMALSRRTSFSLIGMGEAEQVDGRWVTADFFSVYGVHPAMGRDFVPGE
                     DERGVDPVALISAELWQRKFSGAADVLGKVLTLDDKSYTIVGVLPTSFNLYRGID"
     gene            complement(869..>2784)
                     /locus_tag="VN496_05495"
     CDS             complement(869..>2784)
                     /locus_tag="VN496_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF16435.1"
                     /translation="IVLDDNSYTVIGVMPPGFEYFAKADVYVPIELFLDPQSGLADRG
                     SSFTLHAVARLKPGVTVEQANTEMATLGQQLAQEYPKVNEGKSAQADRLQNVMSENLR
                     KTLWVLLAAVGFILLIACINVANLLLVRAAERQKELAVRLALGAGRLRIVRQLLTESL
                     LIATLGGACGLLVGSWMLKGLITLAPPDIPQLSRVSLDKTVLLFTLGIAALTSLLCGL
                     LPAMQSSKADLQTALKEGGRRTTGVGREGMRKALLVAEVSLSLVLLVGAGLLVRSMYN
                     LLHVDLGFNPDNLLTIRLNLSVDRYNRATSRIFYEECVARVQAVPGVETAALAQSLPP
                     EGSYWGSVFIASDKPVPSRADLPELDYLLVSRDYFDTMGIRLLTGRLFTAADTPDTAP
                     VVLINETLARRIWPGEDPIGKRLKNGFPESQMPWREVIGVVNGIKLNGVEHETSMQAY
                     VLFSQVPGTTLGLVIRTHGNPLAPVSSIEEAIHSIDKDLPIYAIWTMDQLLGNSLAQR
                     RLTLVLLASLAALALVLAAVGVYGVTSYAVRQRTHELGIRMALGATARDVLTLILSQA
                     LKLSLLGVALGLGAAYALTRWMETLLFGVRPTDPVTFGVIAAALLGVALLACWIPARR
                     ATKVDPLVALRQE"
BASE COUNT          642 a          858 c          740 g          544 t
ORIGIN      
        1 atcgatgcca cgatacagat tgaaacttgt ggggagcacg ccgacaatcg tgtagctctt
       61 gtcatccagg gtcagaacct tccccagcac atcggctgct ccgctgaact tgcgctgcca
      121 cagttccgca ctaataaggg ccaccgggtc aacgccgcgc tcatcttcac cgggcacaaa
      181 gtcacggccc atcgcagggt gaacaccgta cacagaaaaa aagtccgctg tcacccatcg
      241 tccgtccacc tgttcggctt ctcccatgcc gatcaagctg aaactggtcc ggcgcgaaag
      301 ggccattgct gaaaaagtct ggttctcttt ttgccaatca cgaaaattgg gataagggat
      361 cgcgcccgtt tcgaaattcg gcttgctttg gtgcagcgtt atgagccgct cgggctccgg
      421 gaagggaagc ggattgagca gcacgccgtt gaccaccgaa aagagcgctg cgttggcgcc
      481 aatacccaac gcgagcgtca atagcaccac accggtgagc cctggattct tcactaacat
      541 ccgcgcgcca taacgcaagt cttgaaacat ctcgtcctcc aatctttgtg gctgcaacca
      601 gagcgcatcc caaaacgcgc ccagactgcg gcaaagcaga tcaagtttgg ttttccagtt
      661 gagcttgtcc caatcggcga gcagcagctc acggtgttgc agctcagcct gccattcctg
      721 tttccaatca gctcgtaagc ttcgcggcac gatcacgccg aacagccaga tcagccagag
      781 ccaaaaacga aagcgagttg tgttagttcg atcaaccatc gctttacatg ccgggaacgc
      841 ctacgaaacc ccaaccagaa accagaaact attcctgacg cagcgccacc agcggatcaa
      901 cctttgttgc ccggcgcgcc ggtatccaac acgcgagcaa cgcaacaccc aacaacgcgg
      961 ctgcaatcac tccgaacgtc acagggtcgg tcgggcgaac gccaaacagc agggtctcca
     1021 tccagcgcgt gagtgcgtat gccgcgccta gtccaagcgc cacgcccaac aaggatagct
     1081 tcaacgcctg cgagagaatc agcgtgagca catctcgagc agttgcgccg agcgccatgc
     1141 gaattccgag ttcgtgcgtg cgctgtcgaa ccgcgtagga ggtcacaccg tagacaccga
     1201 ccgccgcgag caccaatgcc agggcggcta agctagcgag caaaacaagc gtcagccgtc
     1261 gttgtgccag cgaattgcct aaaagctggt ccatcgtcca aatcgcgtaa ataggcagat
     1321 ctttgtctat cgaatgaatc gcttcttcga ttgaggaaac gggggcaagt gggttgccgt
     1381 gtgtgcggat tacaagaccc aacgtcgttc cgggtacttg agaaaacagc acataggcct
     1441 gcatcgaggt ctcgtgttcg acgccattga gcttgatgcc gttgacgacg ccgatcactt
     1501 cccgccaggg catttggctt tcggggaagc cgttcttcaa gcgcttgccg attgggtctt
     1561 cgcccggcca gatgcgacgc gccagggttt cgttgattag cacgaccgga gcggtatccg
     1621 gagtatcggc ggcggtaaac aatcgcccgg tcaacaaccg tattcccatt gtgtcgaagt
     1681 agtctcgact cacgagcagg taatccaatt cgggcaggtc ggcgcgcgag ggcacgggtt
     1741 tgtcggaggc gatgaagact gaaccccaat aagatccttc cgggggcaac gactgcgcca
     1801 gtgctgcggt ttctaccccg ggtacagcct gcactcgcgc cacgcattct tcataaaaga
     1861 ttctgctggt cgctcggttg tatctatcca ccgacaggtt caaccgtatc gtcagcaggt
     1921 tgtcggggtt gaagcccagg tcaacgtgca gcaggttata cattgaacgc accaacaacc
     1981 cggccccgac cagcagcaca agcgacagac tgacttccgc gaccagcaac gcctttcgca
     2041 ttccttcgcg gccaactcct gttgtccggc gccctccctc cttaagcgcc gtttgtagat
     2101 cagccttgga cgattgcatc gcaggcaaga gcccgcacag caagctggtc aaggctgcga
     2161 tacccaaagt gaatagcaac acggttttgt ccaagctgac gcggctgagc tgtggaatat
     2221 ccggcggagc cagtgtgatc aaacctttca acatccagct tccaaccagt aacccgcacg
     2281 cgccgcccaa ggtggcaatc aataaactct cggtgagcaa ttgccggacg atgcgcaagc
     2341 gccccgctcc cagcgcgagc cgtaccgcca gttccttttg ccgttcagcc gcgcgcacca
     2401 acaggagatt cgcgacattg atgcaggcaa tgagcaggat gaatccgact gcggccaaca
     2461 agacccagag cgttttccgc aggttttccg acatcacgtt ctgcaaacga tcagcctgcg
     2521 cgcttttacc ctcattgacc ttggggtatt cctgagccag ttgctgtccg agcgtcgcca
     2581 tctcggtgtt cgcctgctcg acagtcacgc ccggcttgag acgcgccact gcatgaaggg
     2641 taaagctgct ccctcgatct gccaggccgg attgtgggtc gaggaagagt tcgatgggca
     2701 cataaacatc cgccttcgca aaatactcga accccggagg catcacgcca atcaccgtgt
     2761 acgaattgtc atccaacact atcg
//
LOCUS       DATMZQ010000169         6413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4902560, whole genome shotgun sequence.
ACCESSION   DATMZQ010000169 DATMZQ010000000
VERSION     DATMZQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4902560"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VN496_05500"
     CDS             <1..1141
                     /locus_tag="VN496_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXF16436.1"
                     /translation="LIDPETTTTWKDGKVQPVGADSSLSTWQEDQWKIGGGTTWGWYS
                     YDKALNLVYYGSGNPSTWNPAQRPGDNKWSMSIWARDVDTGKVKWVYQMTPYDEWDFD
                     GINEMILADIPVKGKQTKALVHFDRNGFGYTLDRTNGALLVAQKYDPAVNWATHVDMK
                     SGRPQVVSRYSTAQNGPDKNTKGVCPAALGSKDQQPASFDPSTGLFYVPTNHVCMDYE
                     PFAVEYVAGQPYVGATLAMYPAPGGKNMGNFITWDAGQGKIVQSHPEKFSVWSGALTT
                     AGGIACYGTLEGYLKCVDAKNINKELYKFKTPSGIIGNVFTYQHKGKQYIGVYSGIGG
                     WAGIGLAAGLEKDADGLGAVGGYKELSNYTDLGGSLTVFALPSTK"
     gene            1226..1612
                     /locus_tag="VN496_05505"
     CDS             1226..1612
                     /locus_tag="VN496_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF16437.1"
                     /translation="MKCLSYAVTALLLYSGVSCLADEPLYKVQDGYKVDAETMKGFKT
                     WRAAACDRCHGANQQGLVGPSLIERLKTLSKEDFVKTVTEGRLEKGMPSFKTSKMVMS
                     NIDHLYAYLKGRSDGAITRSKVEPIE"
     gene            1605..2462
                     /locus_tag="VN496_05510"
     CDS             1605..2462
                     /locus_tag="VN496_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HXF16438.1"
                     /translation="MNSQVAAALLVLTLAAIAWTVAAEEEPFEKPRALRVCDDPNNLP
                     FSNDKGEGFENKIAEILARKLDLPLEVFHYPERINIVRNTIRYKLPNESDYRCDLLTG
                     VPSDWTAVAATKPYFRSTYVLVYAKGGPIDVGSVEQFLKLDKATLKRLKIGVYERTPA
                     VDWMKKHDLLDQAVPYRLLNADPSHYPGKIIDDDLAKGKVDAVIVWGPIGGYFAGRVR
                     SPELVVLPLKPEPGIRLDFSISMGMRQGENAWRAMIQKALDDSRDEINKILSEYNVPV
                     VDDKGQISR"
     gene            2524..2868
                     /locus_tag="VN496_05515"
     CDS             2524..2868
                     /locus_tag="VN496_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16439.1"
                     /translation="MSRKQQLLVLTLVSAAWPAIANDFPTVERVEFVLECMRNHKGGE
                     FELLNKCSCAIDRLAEKYTHDDFVEAQTMAKAVTIAGERGSTLRDNEEAQKAARQYRA
                     DIKDAANACFLH"
     gene            2849..3676
                     /locus_tag="VN496_05520"
     CDS             2849..3676
                     /locus_tag="VN496_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HXF16440.1"
                     /translation="MPVSCTDSKPSWRRFVLMLALSFGAGVAPAADDSANPDTQIWRN
                     VRADLFGERAIKDDSATGRIRLELPARAADAAVVPLAVRITPRPGEARPNKIYLIIDR
                     NPSPIAGIFEFGKGSGSTDLETRVRIEEYTWVRVIAEAADGSLLATQRFIKAAGGCSA
                     PAGKSLAERLAGMGQMKWRIDPPADPSSPATVQLMIRHPNNSGLAMDQLTRLYDPPYY
                     VRNVRVTRGNELVVAADVDFSISENPAFRFTVMPGPVGPLHALVIDSNEQVFESSAP"
     gene            complement(3673..4590)
                     /locus_tag="VN496_05525"
     CDS             complement(3673..4590)
                     /locus_tag="VN496_05525"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXF16441.1"
                     /translation="MTPLSFEGVYPILATPFDGNENLDLESLDRLVRFMAKLDVEGIT
                     ILGVLGEANRLMDGERETLIRTAVSAAGSMPVIVGTSHSGTRAARDLSQMAENLGARA
                     IMVTPHAEPVPNDDRIVEYYRTIADGVQIPIVVQDHPASTQVHMTAPLLLRIIREVPR
                     VASIKEEAVPTAPKIRALAAGMQERKVPVLTGLGALYGLFDLEAGSRGFNTGFAFPEV
                     LTAMVKSARTGDWKKVRKLYTRFLPLIVFEQQPGVAVRKEIFRLRGVLESAKVRHPGG
                     ALHPATADQLRSLLDAILPGEDLSQPIAL"
     gene            4691..5455
                     /locus_tag="VN496_05530"
     CDS             4691..5455
                     /locus_tag="VN496_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXF16442.1"
                     /translation="MEMPVNQLKRALRAGRTQTGCWLSLASHVSAEICASAGFDWVLV
                     DTEHAPADLQNVHHQLHAIAAYPVSALVRAYWNDTVLIKRLLDIGVQSLLLPYVQNEE
                     EAKRAVAAVRYPPKGIRGVSANSRANRFGRVKDYFSHANEEICLMVQVETRAAVSEIE
                     KMAAIDGIDGIFIGPQDLAADFGHLGNPGHPEVQAAMADALTRIKKTGKVAGILAFAE
                     PDARRWIEHGAQFVAITSDQFLLVRESAAIASRFKT"
     gene            complement(5452..5886)
                     /locus_tag="VN496_05535"
     CDS             complement(5452..5886)
                     /locus_tag="VN496_05535"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HXF16443.1"
                     /translation="MDFELINEMRDYETAESRSALQQRLLLIDEALRNEFQQRGLYQV
                     VDNAPAAKLIAEEKSLHDLRQCNGCEIDIGRALQADVVVLGWVQKVSNLILNVNIEVK
                     DVASGNTLYTKSVDLRSNSDRSWLRGIRYMVDSIEEKRQYQR"
     gene            complement(5991..>6413)
                     /locus_tag="VN496_05540"
     CDS             complement(5991..>6413)
                     /locus_tag="VN496_05540"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HXF16444.1"
                     /translation="DEFHSAQNIEKMRNGIALQDADRWPWLDSMNAMLLEKQAANQSI
                     LLACSALKQKYRDRLADGCRNLHWVYLKGEFDLIRGRLEARKNHYMKAGLLESQFAAL
                     EEPRDAVVVSVIETPDAIADRILRDLKIDPILTTGSSR"
BASE COUNT         1393 a         1798 c         1881 g         1341 t
ORIGIN      
        1 gctgatcgat cccgagacca cgacgacctg gaaggacggc aaagtccagc cggtcggtgc
       61 agattcatcg ttgagcacct ggcaagaaga ccagtggaaa atcggcggcg gcaccacgtg
      121 gggttggtac agctacgaca aggcactgaa tctggtttat tacggctcgg gcaatccgag
      181 tacgtggaac ccggcgcagc gtcccggaga caacaaatgg tcgatgtcga tctgggcgcg
      241 tgatgtggat accggcaagg tgaagtgggt ttatcagatg acgccgtacg acgagtggga
      301 tttcgacggc atcaacgaaa tgatcctcgc cgacatcccc gtcaagggca agcaaaccaa
      361 ggcgctggtg cattttgatc gcaacgggtt cggttacacc cttgatcgca ccaacggcgc
      421 attgctcgtc gcacagaaat atgatccggc agtgaattgg gcaacgcacg ttgatatgaa
      481 atccggtcgc ccacaggtcg tttcccggta ttcgacggca cagaacggcc cggacaagaa
      541 taccaaaggc gtttgtcccg cggcgctcgg ttccaaggat cagcaacccg catccttcga
      601 cccgagtacg ggtttgttct atgtgccgac gaaccacgtc tgcatggact acgaaccgtt
      661 tgcggttgaa tacgtagccg ggcaacccta tgtcggcgcg acgctcgcga tgtacccggc
      721 gccgggcggc aaaaacatgg gcaacttcat cacgtgggac gccgggcaag gcaagatcgt
      781 gcaaagccat cccgaaaaat tttcggtctg gagcggtgcg ttgaccaccg ccggtggaat
      841 cgcgtgctat ggcaccctgg agggttacct gaaatgcgtg gatgcgaaaa atatcaacaa
      901 ggaactgtat aagttcaaaa cgccgtccgg catcatcggt aacgtgttca cctatcagca
      961 caagggcaag cagtacatcg gcgtttattc tggcattggc gggtgggccg gcatcggcct
     1021 cgcagccggt ctggaaaagg atgccgacgg ccttggcgcc gtaggtggct acaaggagtt
     1081 gtcaaactat accgatctcg gtggttcgct gacggtattt gcgctgcctt cgacgaaata
     1141 aaccgaagcg gcacatcgtg cgttattgga ggcgtcccgg ccggtgggca ggggcgcctt
     1201 cttattccct gaaccggagc cggaaatgaa gtgtttgagc tacgcggtta ctgcattgct
     1261 gttgtattcc ggcgtttcat gcctcgccga tgaaccgctt tacaaggtgc aggatggcta
     1321 caaggtggac gccgaaacga tgaagggatt caagacatgg cgcgcggccg cgtgtgaccg
     1381 ctgccacggc gccaaccagc aaggtctcgt tggaccctca ctgatcgaac gtctgaagac
     1441 gttgtctaaa gaagacttcg tgaagacggt taccgagggc cgcctggaaa aaggcatgcc
     1501 gagcttcaag accagcaaaa tggtgatgtc caatatcgat catctctacg cctatctgaa
     1561 aggacgttct gacggtgcaa tcacgcgttc aaaggtcgaa ccgattgaat agccaggtcg
     1621 ccgctgcgct attggtcctc acgcttgcgg cgattgcgtg gacagtggcg gccgaggaag
     1681 agccgttcga gaagccgcgc gcgctgcggg tttgcgacga tccgaataat ctcccgttct
     1741 ccaacgacaa gggcgaaggt ttcgagaaca agattgctga aatcctggcg cgcaagctgg
     1801 acttgccgtt ggaggttttt cattatcccg aacgcatcaa catcgtcaga aacaccattc
     1861 gctacaagct gccaaatgaa tccgattacc gctgcgattt gctgacgggt gtgccctcgg
     1921 attggaccgc agtcgcggcg acgaagccgt attttcgctc gacttatgtg ctggtgtatg
     1981 caaaaggcgg ccccattgat gttggcagcg tcgagcagtt cctgaagctc gacaaagcaa
     2041 cgctcaaacg gctgaagatt ggcgtctatg aacgcacgcc agccgtcgac tggatgaaaa
     2101 aacatgatct tctggatcag gccgtgccgt accggctgct gaatgccgat cccagtcact
     2161 atcccgggaa aattattgat gacgatcttg ccaaaggcaa ggtggacgcg gtcatcgtgt
     2221 ggggacccat cggtggctat ttcgcggggc gcgtcaggtc cccggagctt gtcgtattgc
     2281 cccttaaacc ggagccgggt attcgtcttg atttcagtat atcgatgggc atgcgccagg
     2341 gagaaaacgc ctggcgtgca atgatccaga aggcgctcga cgatagtcgt gacgagatca
     2401 acaagatcct tagcgagtac aacgttccgg tcgtggacga caagggtcaa atatcgcgct
     2461 agcaagcgcc gacagctgat ccggacttcg tactatgcct gccgcaattt cagaggtaca
     2521 gcaatgagca gaaagcaaca gctgctggtc ctgacactcg tgtccgctgc ctggccggcc
     2581 attgcgaacg atttccctac tgtcgagcgc gtagagtttg tgcttgaatg catgcgcaac
     2641 cacaaaggcg gcgaatttga gttgctaaac aagtgttcgt gtgcaataga tcgccttgca
     2701 gagaaataca cacacgacga tttcgtcgaa gcgcagacca tggccaaggc agtcacgatt
     2761 gcgggcgagc gcgggtccac attgcgcgac aacgaggaag cacagaaggc ggcccggcaa
     2821 taccgcgcag atatcaaaga tgctgcgaat gcctgtttcc tgcactgaca gcaagcctag
     2881 ctggcggcgc tttgtgctca tgcttgccct gtcgtttgga gctggagtgg cgcctgcggc
     2941 cgatgacagc gccaatcccg atacgcaaat ctggcgcaac gtgcgcgctg atctgttcgg
     3001 cgagcgagca atcaaggatg acagcgccac cgggcggata cgtctggaac tgccggctcg
     3061 cgccgccgat gcggcagtcg ttccattggc ggttcgcatc acgccaaggc cgggcgaagc
     3121 gcggccaaac aaaatatacc tgatcatcga tcgcaacccc tcacccatcg ccgggatttt
     3181 cgaattcggc aagggcagcg gaagtactga cctagaaaca cgggtcagga tcgaagaata
     3241 cacttgggtt cgtgtcatcg ctgaggcggc ggatggctcg ctgttggcga cgcagcggtt
     3301 catcaaggcg gccgggggct gttcggctcc cgctggcaag tcactcgccg agcgactggc
     3361 ggggatgggg caaatgaaat ggcgcatcga ccctccagct gacccgtcct cgccggcaac
     3421 ggttcagctc atgatccgtc atccgaacaa ttccgggctg gcgatggacc agttgacgcg
     3481 gctgtacgat ccgccttatt acgtacggaa tgtacgcgtc acgcggggta acgagctggt
     3541 cgttgcggcc gatgtggatt tttcaatcag cgagaatccg gcttttcgct tcactgtgat
     3601 gccaggtcct gttggtcccc tgcatgcgct tgtaatcgat tccaacgaac aggtgttcga
     3661 aagctcggcg ccttaaagcg caatgggttg cgaaaggtct tcgccgggca agattgcgtc
     3721 cagcaggctc ctcagctggt cagccgtcgc gggatgcagt gcgcctccag gatggcgcac
     3781 cttggccgac tcgagcacgc cacgcaaccg gaagatttcc ttacgcaccg ctacccccgg
     3841 ttgttgttcg aacacaatca gcggcaaaaa gcgcgtgtag agcttgcgca cttttttcca
     3901 gtcgccggtg cgcgctgatt tcaccatggc ggttaatact tcggggaaag cgaagccagt
     3961 gttgaatcct cgggatccgg cttccagatc gaacaatccg tacagcgcac cgagccccgt
     4021 cagcaccggc accttgcgct cctgcatccc tgcagcaagc gcgcgtatct tcggcgcggt
     4081 cgggacggct tcttccttga tgctcgcgac gcgaggcact tcccggatga tgcgaagcaa
     4141 aagtggcgcg gtcatatgca cctgcgtcga agccgggtga tcctgcacga cgatcggaat
     4201 ctgcacccca tcggcaatcg tccggtaata ctccactatg cgatcgtcgt ttgggaccgg
     4261 ctcggcgtgc ggcgtgacca ttatggctcg cgcgccaaga ttctccgcca tttgcgaaag
     4321 gtcgcgcgct gcccgcgtgc cactatggct ggtaccaaca atcaccggca tggaaccggc
     4381 ggccgacacg gcggtccgga tcagggtttc gcgttcgccg tccatcaaac gattggcttc
     4441 gcccagtacg ccgaggatgg taatgccttc cacgtcgagc ttggccatga accggaccag
     4501 ccggtccaaa gactccagat cgagattttc gttgccatcg aacggcgtgg ccaagatcgg
     4561 atacacgcct tcgaaactca atggtgtcat gcggattctc cagggagcgc gcattgtcag
     4621 ccgcccgctg aagggtgtca aagagcggtt acaatcgcgc gtcggttcaa aacaatatcg
     4681 ggagaacacc atggagatgc cggtcaatca gctcaagcgt gcattgcgcg cgggccgcac
     4741 gcaaaccggt tgctggttga gcctcgcaag ccatgtgtct gccgaaattt gcgcctctgc
     4801 cggttttgac tgggtactcg tggataccga gcatgcccct gccgatctgc agaatgttca
     4861 tcaccaattg cacgctatcg cggcctaccc ggtgtcggca ttggtgcgtg cctactggaa
     4921 tgacacagtg ctgatcaaac gcttgctgga cattggcgtg cagtccctgc ttctgcccta
     4981 cgtgcagaac gaggaagagg ctaagcgagc agtggccgcg gtgcgctacc ctccaaaggg
     5041 catacgtggg gtttcggcaa attctcgtgc caaccgcttt ggccgcgtta aggattactt
     5101 ctcgcatgcg aacgaggaaa tatgtctgat ggtgcaggta gaaactcgcg cggccgtatc
     5161 ggagatagaa aagatggcgg cgatcgacgg catagacgga attttcatcg gcccgcagga
     5221 ccttgctgct gatttcggcc atcttggcaa tccggggcat ccagaagtac aggccgcgat
     5281 ggccgatgcg ctaacgcgca tcaaaaagac gggcaaggtt gcaggcatcc tggcttttgc
     5341 agaaccagac gcccggcgct ggattgaaca tggcgcccaa ttcgtggcaa ttacctccga
     5401 ccagtttttg ctggtgcgcg aaagcgcggc tattgcctcg cgcttcaaaa cctaacgctg
     5461 gtattgccgt ttttcttcga tgctatcgac catgtaacgg atgccacgca accatgatct
     5521 gtcggaattg ctgcgcaaat ccacggattt ggtatagagc gtgttcccgg atgccacgtc
     5581 ctttacttcg atgttgacgt taagaatcag gttactgact ttctgcaccc agcctagaac
     5641 aaccacgtcg gcctgcaatg cacggccgat gtcgatttcg caaccgttac actggcgcag
     5701 gtcatgcagc gacttttctt ccgcaatcag tttcgcggcc ggcgcattat caacaacctg
     5761 gtacaagccg cgctgctgga attcattgcg caaggcttcg tcgatcaaaa gcaggcgttg
     5821 ctgtagtgcc gatcgggact cggctgtctc gtagtcgcgc atttcattga tcagttcgaa
     5881 gtccatgact gcagcgcgct tcggcgccgg cgatcccgac tcccgggctt gcgattgggc
     5941 cccgcagaat aaacacaacc aaaacccaat tgcgatgaat agcagtcggc tcatcggctg
     6001 gagccggtcg ttagaatcgg gtcgatctta aggtcgcgca ggatacgatc ggcaatcgca
     6061 tctggagttt cgataacact gacgaccact gcgtcccgtg gttcttccag cgcggcaaac
     6121 tgcgattcaa gcagtcccgc tttcatgtaa tggttcttgc gtgcttccag gcgaccgcgg
     6181 atcaggtcga actcaccttt caggtagacc cagtgaagat tccggcaacc gtcggcaagg
     6241 cggtcgcgat atttctgttt taatgccgaa catgccagca ggatgctttg atttgccgct
     6301 tgcttctcca gcagcatggc gttcatgctg tccagccacg gccatcggtc cgcgtcttgc
     6361 agggcaatgc cgttgcgcat cttttcgata ttttgcgccg agtggaattc atc
//
LOCUS       DATMZQ010000170         6290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1171558, whole genome shotgun sequence.
ACCESSION   DATMZQ010000170 DATMZQ010000000
VERSION     DATMZQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171558"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..2423)
                     /gene="speC"
                     /locus_tag="VN496_05545"
     CDS             complement(60..2423)
                     /gene="speC"
                     /locus_tag="VN496_05545"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HXF16445.1"
                     /translation="MIPRSVKSPTIPVPFHKLLKVVAIVDKNDPQTRQLLELIAADNF
                     QIEVTDSFERDVSEDAAVGAYIALVDGDRREPARKLAQSVRNIGFATPLWALANSHRI
                     SDLAVLGNLGEVDGYIYLGQQTPAFYAKQVVASLVKYGKGLLPPFFGGLVAYDGEANI
                     AFDCPGHQGGQFYSKSPAGQLFFKHFGESVFRNDLCNADVDLGDLLIHEGPAVEAQRY
                     AAKVFGADKTYFVLNGTSTSNKVVTNAVLRRGDLVLFDRNNHKSLHQGALVQAGAIPI
                     FLPTARNPFGMIGAVDWSAWDEAYLREQIRANPLIKDKERHKGERPFRLACIQLATYD
                     GTIYNVRKVMEKIGHLCDYVLWDEAWIGYNAFHPLFEDHSPMRIQNLSPQMPGLFSTQ
                     SVHKQGAGFSQASQIHKRDIHIRGQKRYVEHKRFNESFLMNASTSPFYPLFASLDVNA
                     KIHDGKAGEMLWDRCIELGIETRKKLREFGRYYARTGRTSEEQWFFDPFVPDVVTLRN
                     SKFAKDATDVAWEELPTEVIKREQQCWNFHPKSKWHGYAGYGDEYAMVDPNKLALLTP
                     GIDRRSGDYLNWGVPATVVANYLREERVVPEKCDLNSILFLMTPAEDESKMNTLVAKL
                     VKFKNLWDRDAPIAEVLPTLYAAHGERYAGYTLRQVCKEMHDFYRKANVKELQRQCFR
                     ASSFPELAMSSEDAYAALVANDVDYIPLDQIRGRISATLALIYPPGIGVVVPGERWDA
                     KAQPMLDYFLAFQESFNRFPGFNYEVQGVYQERIDGRIKFHTYVVKE"
     gene            complement(2420..4765)
                     /locus_tag="VN496_05550"
     CDS             complement(2420..4765)
                     /locus_tag="VN496_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXF16446.1"
                     /translation="MLEGKFAPLRVRVLVIDDELTQDTAEGRAARGLVSGLEVRNLEV
                     VQAASAADGMTVVTSDAAIHCVLMDWTLGDDDEKTHEKAKGVLNYIRGRNARIPIFLM
                     AERRDSARLPVEVIQQVDEFIWTLEDTAAFVSGRVEAAARRYIEEMLPPFAAALGRFA
                     RVHEYSWHTPGHTGGTAFLKSPAGRVFYDYFGENLFRSDLSISVGDLGSLLDHTGPIG
                     ESEKYAARVFGAHRTYCVTNGTSTSNRIIFMAAVARDQIALCDRNCHKSIEHGLTMTG
                     AIPTYYVPLRNRWGIIGPIHPDQFTKASLKKAIASNPLVHEGVDPKPVYSVVTNSTYD
                     GLCYNAVAVEELLGDSVDRVHWDEAWYGYARFNPIYRDRFGMRGAPADHTGPTVFATH
                     STHKLLAALSQASYLHIRDGRSPISHARFNEAFMMHASTSPLYTIIASNDITSAMMDG
                     QGGLTLTNESIQEAVAFRQTMGRIHAHFAKKKEWFFNTWNADEVALPGNRKRVAFHEA
                     PPELLASEPNAWVMHPGDDWHGFDSLPDDYCMLDPVKVSIVTPGVGRNGKFETFGIPA
                     TLITAYLDRHGIQVEKTTDFTVLFLFSFGITKGKWGTLVTALLDFKRDYDANLPLELA
                     IPRLCASHPARYGAMGLRDLATEMFEQLKSARQTHWLAQAFSTLPTPRLTPADAYREL
                     VLDNIEHVALEDMADRVLATSVVPYPPGIPMLMPGESAGAADGPYLSYLRALQGWDAR
                     FPGFGHDTHGVENKEGRLYMQCLRKDAKAQPAPRKRGGARR"
     gene            complement(4821..6128)
                     /locus_tag="VN496_05555"
     CDS             complement(4821..6128)
                     /locus_tag="VN496_05555"
                     /inference="COORDINATES: protein motif:HMM:NF039990.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HXF16447.1"
                     /translation="MKQRAVLFAIAFLVSGGAQAQTEDSAKTIQDLQNRVKALEQQKQ
                     TSPAGQNAAGAPVVSPGATPEEGAPDPSKARIEIYGQIMLDAIYDFKRVDPNWNATLR
                     PSTIPVHCPGDAGCGKNGETIMSIRQSKLGFKAYIPTSSGELTTELDFDLFSSGGGNT
                     EARVLNAWGQLGMFGAGQYYTVFMDIDTFPNTIDYWGPSGMVFIRNPQLRITPIKRDG
                     MTVAVSLEAPNSAIDTGKVSDVDPALASGITGRNRYPDLAASVRLDRGWGHVQAAGLL
                     RLVSFETPSNPGSEPSGQETGYGLNLSTAVNTFGKDRVVGQLVVGRGIASYMNDGGVD
                     LAPSASLQAETVKTVGWFVYYDHYWNERWSSSAGVSQHVQDNTDGQLGNAFKKGSYAS
                     ANVLFYPVKNVTTGAELLWGKREDKDGSSGTDNRIQFSTKYTF"
BASE COUNT         1218 a         1877 c         1845 g         1350 t
ORIGIN      
        1 gagcgcgtcg tcgaccatca ggacgcgcgg acggatagga ccggcggcgg gtgtgagcat
       61 tattccttca ccacgtaggt atggaacttg atgcgcccgt cgatccgctc ctggtacacg
      121 ccctgcactt cgtaattgaa accgggaaag cggttgaacg actcctgaaa ggcaagaaaa
      181 taatcgagca taggctgcgc ttttgcgtcc catcgttcgc cgggcacgac cacgccaatg
      241 ccgggcggat aaatcagcgc caaggtcgcg gaaatgcggc ccctgatctg atccagtgga
      301 atgtagtcca catcgtttgc gaccagtgcg gcataagcgt cttccgacga catcgcgagt
      361 tcgggaaaac tcgatgcgcg gaagcattgc cgctgcaatt ccttgacatt ggccttgcga
      421 tagaaatcgt gcatttcctt gcagacctgt cgcagcgtat aaccggcgta gcgctccccg
      481 tgcgccgcat agagcgtcgg cagcacttcg gcgatcggtg cgtcgcggtc ccacaagttc
      541 ttgaacttca ccagcttcgc gaccagggtg ttcatcttgc tttcgtcttc cgccggggtc
      601 atcaggaaca ggatgctgtt cagatcacat ttttccggaa cgaccctctc ctcgcgcagg
      661 taattggcga ccaccgtggc ggggacaccc cagttcagat agtcaccaga cctgcgatcg
      721 attcccggtg tgagcagtgc aagcttgttg gggtccacca tcgcgtattc gtcgccgtag
      781 cccgcgtaac cgtgccattt gcttttggga tggaagttcc agcactgctg ctcgcgtttg
      841 ataacttccg tgggcaattc ttcccatgcg acatcggttg catccttggc gaatttcgag
      901 ttgcgcagtg tcacgacgtc cggaacgaaa ggatcgaaga accattgctc ctcgctggtc
      961 cggccggtgc gcgcgtaata gcggccgaac tcgcgcagct tcttgcgggt ttcgatcccg
     1021 agttcgatgc agcggtccca caacatctcg cctgccttgc catcgtggat cttggcgttc
     1081 acgtcgaggg acgcaaacag cggatagaaa ggcgacgtcg aagcattcat caggaacgat
     1141 tcgttgaagc gcttgtgctc gacgtagcgc ttctggccac ggatatggat gtcgcgtttg
     1201 tgaatctgcg aggcctgcga aaaaccagcg ccttgtttgt gcaccgattg tgtggaaaac
     1261 agtcctggca tttgcggcga caggttctgg atgcgcatcg ggctatggtc ttcgaacagc
     1321 ggatggaacg cgttgtatcc gatccaggcc tcgtcccaca gcacgtaatc gcacagatgt
     1381 ccgattttct ccatcacctt gcgcacgtta tagattgtcc cgtcgtaggt ggccagctgg
     1441 atgcaggcca gcctgaacgg gcgttcgccc ttgtggcgct ccttgtcctt gatgagcgga
     1501 ttcgcccgga tttgctcgcg cagataagct tcgtcccatg cgctccagtc gactgccccg
     1561 atcatgccga atgggttgcg tgccgtaggc aggaagattg gaatcgcgcc cgcctgaacc
     1621 agtgcaccct ggtgcaatga cttgtgattg ttgcggtcga acagcaccag gtcgccccgg
     1681 cgcaacaccg cattggtaac tactttgttc gatgtactcg tgccgttcag cacgaaatag
     1741 gtcttgtccg cgccgaacac tttcgcggcg tagcgctgcg cttctacggc ggggccctca
     1801 tggatcagca gatcgccaag atcgacgtcg gcattgcaca gatcgttgcg gaacacgctt
     1861 tcgccgaaat gcttgaagaa cagctgcccg gccggagact tgctgtagaa ctggccgcct
     1921 tgatggccgg ggcagtcgaa cgcgatgttt gcttccccgt cataggccac cagcccgccg
     1981 aaaaacggcg gcagcaggcc tttgccgtat ttgaccaggc ttgccacaac ctgcttcgcg
     2041 taaaacgcag gtgtctgctg gccgaggtaa atatagccgt cgacttcgcc aagattgccg
     2101 aggacggcaa gatcagagat gcgatgagaa ttcgccaggg cccatagcgg cgtggcgaaa
     2161 ccaatgttgc gcaccgattg cgcgagcttg cgcgcaggtt cacggcggtc tccatccacg
     2221 agtgcaatgt acgcaccgac cgcggcatct tcggaaacat cccgctcgaa actgtcggta
     2281 acctcaatct gaaaattgtc ggcggcgata agctcgagaa gctgcctggt ctggggatcg
     2341 ttcttgtcta cgatcgccac cactttgagc agtttgtgga aggggaccgg tattgtgggc
     2401 gatttgactg agcgtggaat catcggcgcg ctcctccgcg cttgcgcggc gctggttgtg
     2461 ccttcgcatc ctttctcagg cattgcatat acaggcgccc ttccttgttc tcgacgccgt
     2521 gcgtgtcgtg tccaaatccg ggaaagcgcg catcccagcc ctgcaatgca cgcaggtagc
     2581 tcagatacgg accatctgct gcaccggcac tctcgccggg catcagcatc ggaatgccag
     2641 gcggatacgg caccacactg gtggccaata cccggtcggc catgtcttcg agtgccacgt
     2701 gctcgatgtt gtccagtacc aattcgcgat acgcgtcggc aggcgtcagg cgcggcgtcg
     2761 gcagcgtcga gaatgcctgc gcaagccagt gtgtctgccg ggcgcttttc aattgctcga
     2821 acatttcggt ggcgaggtcg cgcaatccca tggcgccgta gcgggccgga tgcgaagcgc
     2881 acaagcgcgg aatcgccagt tcgagcggca ggttcgcatc gtagtcgcgt ttgaaatcga
     2941 gcagcgcggt aaccagcgtg ccccacttgc ctttggtgat gccgaaggag aacaggaaca
     3001 acaccgtgaa atcggttgtc ttctcgacct gaatgccgtg ccggtcgagg tacgcggtaa
     3061 tcagcgttgc cggaatgccg aacgtttcaa acttgccgtt tctgccaacc cccggcgtga
     3121 cgatcgacac ctttaccgga tccagcatgc agtaatcgtc gggaagggaa tcgaatccgt
     3181 gccagtcgtc gcccggatgc atcacccatg cgtttggctc gcttgccagc agttcgggcg
     3241 gcgcttcgtg gaatgcaacg cgcttgcgat tacccgggag cgcaacctca tcggcattcc
     3301 aggtgttgaa gaaccactcc ttctttttcg caaaatgcgc gtggatgcgg cccatcgtct
     3361 gccggaacgc gaccgcctcc tggatcgact cgttagtcag cgtcaatccg ccctggccat
     3421 ccatcatcgc cgacgtaatg tcgtttgacg cgatgatcgt atacagcggg gaggtcgagg
     3481 catgcatcat gaaggcctcg ttgaagcgcg catgcgaaat cggactgcgc ccgtcgcgga
     3541 tgtgcagata cgaggcttgc gataatgccg caagcagttt gtgcgtggag tgcgtggcga
     3601 acaccgtcgg tcccgtatgg tcggcaggcg ctccgcgcat gccgaagcga tcgcgataga
     3661 tcgggttgaa gcgcgcgtag ccgtaccatg cttcgtccca gtggacgcga tcgacgctgt
     3721 cgccgagtaa ctcctcgacc gccaccgcgt tatagcaaag accgtcataa gtcgagttcg
     3781 tcaccacgct atagaccggc ttcggatcga cgccttcgtg gactagcgga ttggacgcga
     3841 tcgctttctt cagcgaggcc ttcgtaaact ggtccgggtg gatcgggccg atgatgcccc
     3901 agcgattgcg cagcggcacg taatacgtcg ggatcgcacc ggtcatggtg agcccgtgct
     3961 cgatcgactt gtggcagttg cgatcgcaca gcgcgatctg gtcgcgcgcc acggcggcca
     4021 tgaagatgat gcggttcgag gtcgaggtgc cgttcgttac gcaatacgta cgatgcgcgc
     4081 cgaacacgcg cgcggcgtac ttctcggatt caccgatcgg gccggtgtga tcgagcagcg
     4141 agcccagatc cccgacgctg atggaaaggt cggatcggaa cagattttca ccgaagtagt
     4201 catagaacac gcggcctgcc ggcgacttca ggaatgccgt gccgcccgta tgccccggtg
     4261 tgtgccacga atattcatgc acgcgtgcaa aacgtccgag cgccgccgcg aatggcggca
     4321 acatttcctc gatgtagcgc cgcgctgcag cttcaacgcg gccgctgacg aacgccgccg
     4381 tatcctcgag cgtccagatg aattcatcga cctgctgaat cacttcgacc ggcagccgtg
     4441 ccgaatccct gcgctcggcc atcaggaaaa tcggaatacg cgcattgcgg ccgcgaatgt
     4501 agttcaggac ccctttggct ttctcgtggg tcttctcatc atcatcaccg agcgtccagt
     4561 ccatcagcac gcagtgtatg gcggcatcgg aagtgacgac cgtcatgcca tcagccgccg
     4621 aagcggcttg cacgacctcg agattgcgca cttcaaggcc gctcaccagc ccgcgcgctg
     4681 cgcggccctc ggccgtgtct tgcgtcagtt catcgtcgat gacgaggacg cgtacgcgta
     4741 gcggagcaaa tttgccttca agcatagtcg atgacctcag tatttttacc ggccgccacg
     4801 cagcggccga atggcagccg ctagaacgta tacttggtgg agaactggat gcgattgtcg
     4861 gtgccggacg agccgtcctt gtcttcgcgc ttgccccaca acagttccgc accggttgtc
     4921 acattcttta ccggatagaa cagcacgttg gcagaggcat agctgccttt cttgaacgcg
     4981 ttgccgagct gcccgtcggt attgtcctga acgtgctggc tgacgccggc cgagctgctc
     5041 cagcgctcgt tccagtagtg gtcgtagtaa acaaaccatc caacggtctt gaccgtttcc
     5101 gcctgcaagc tggcactcgg cgccagatcg acgccgccat cgttcatgta actcgcgatg
     5161 ccgcggccga cgacgagctg gccaacgacg cggtcttttc caaacgtgtt cacagccgtg
     5221 ctcaagttca aaccatagcc ggtttcctgc ccggaaggtt ccgatccggg attggaaggc
     5281 gtttcaaagc tgaccaggcg cagcagtccg gccgcctgca catggcccca gccgcggtcg
     5341 aggcgcacgc tggcagcgag atccggataa cgattgcggc ccgtgatgcc tgatgccagc
     5401 gccgggtcga catccgatac tttgccggta tcgatcgccg aatttggtgc ctcaagagac
     5461 actgccacgg tcatgccgtc acgcttgata ggcgtgatgc gcagctgcgg attgcggatg
     5521 aataccatcc cgctcggccc ccaatagtcg atggtgtttg gaaaagtgtc gatgtccatg
     5581 aacaccgtgt agtactgccc cgcaccgaac atgcccaact gtccccatgc attgagcacg
     5641 cgcgcctcgg tattgccgcc gccgctgctg aacaaatcga aatccagctc ggtcgtcaat
     5701 tcacccgacg aggtcgggat gtatgccttg aatccgagct tcgattgccg gatgctcatg
     5761 atcgtctcgc cgttcttgcc gcaacccgcg tcaccgggac agtgcaccgg aatcgtggag
     5821 ggccgcaatg tcgcgttcca gttcgggtcg acgcgcttga agtcgtaaat cgcgtccagc
     5881 atgatctgtc cgtatatttc aatacgcgcc ttggacggat ccggcgcgcc ctcctcaggt
     5941 gttgcgccgg gtgaaactac gggcgcaccc gccgcgttct ggcctgcagg cgaggtttgc
     6001 ttctgctgtt caagtgcttt gacgcggttt tgcagatcct ggattgtttt tgcagagtct
     6061 tcagtttggg cttgcgcacc gccactcact agaaatgcga ttgcaaacaa taccgcgcgt
     6121 tgtttcatag ttattttttc tcccccaaaa ttgactcacc cgttcgcggg cggatgctgc
     6181 gggcgttggc gcccggagca tcattatgtg cggcgaatta tttctaaagc atgacttcag
     6241 gtattgactt caagtagtcg gccatgctga acaacgcgat caattctagc
//
LOCUS       DATMZQ010000171         7091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_135739, whole genome shotgun sequence.
ACCESSION   DATMZQ010000171 DATMZQ010000000
VERSION     DATMZQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135739"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VN496_05560"
     CDS             <1..768
                     /locus_tag="VN496_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16448.1"
                     /translation="AARNVASLYGRFPVASLQVVVSPTPRGRGPVPWAYVSRGGGPAV
                     HLFINPARTPAEFERDWSLTHEMAHLFLPYVDARDAWLFEGLPTYLQNVLMARGGAIS
                     VEEAWRRMHAGFERGARTAPDVSFARANERIGQGNIYLRVYWGGAAMMLAADLQLRAQ
                     TGGKQSLATALEQLSRCCLGDNRRWGAQEIIERLDRLSGTTVFTDIVRAQFAANGYPD
                     YADILSRAGVKVEGDHVELDASAPLAAEREALMHAAH"
     gene            827..2287
                     /locus_tag="VN496_05565"
     CDS             827..2287
                     /locus_tag="VN496_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF16449.1"
                     /translation="MNPEELCFLPAHELAHLIRSRELSAAEVMDAHIAQIERVNPAVN
                     AIVTFLPERAQESARAVDAALKRGQEPGPLAGLPVAHKDLTPTKGIRTTFGSPIFKDF
                     VPDADALIVERQRAAGAITVGKTNTPEFGAGSQTFNPVFGPTRNPYDLGKTCGGSSGG
                     AAAALASGMLPLADGSDLGGSLRNPASFCNVVGLRPSSGRVPHWPSVSAWFSLAVVGP
                     MARTVQDIALFMASIAGPDARSPISLQDPAALFSRPLERDFKDVRIAWSTDLGGLPME
                     PEVIRVMQSQRQTFADLGCIVEEATPDLRDAHEIFSVLRAWNFYLSYGELFKTKRALM
                     KDTVIWNIEQGARLTGPEVGTAEVKRTELFHRMRQFMKKYDFLVAPVAQVLPFAVDIP
                     YPNRIGDVALDTYIDWMRSCYYVTVTGAPALSVPCGFSSSGLPVGLQIVGRYQDDFGV
                     LQLGAAFEQATGCWKRRPAIGQNNPELRTLNHALKI"
     gene            2271..3101
                     /locus_tag="VN496_05570"
     CDS             2271..3101
                     /locus_tag="VN496_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16450.1"
                     /translation="MHSRFDPLWRFTTLLVIAALVIPAKAQTTPKPAPSARKDRVFLK
                     DIEGIWINEAYLGALAALKSPHAAAKKTPPLVIAIRREGAAYPIVITDFQKASLQAVL
                     DIEPDGKPNAYRLVVAADDKPTSSNEVKYIRFEAVKNGQGKFDRLHVAEPDFMKGRWA
                     DYVPIAGELSPQLNRFVIAGKYRDDKGQPWEFTESGDATWPQQKFNYELSLNDPGASC
                     DYLQSESTAAEPQKNSVEQDKKRYGYAWKAGKLSIFQARLAGKKVVCESKPLAVLTPH
                     "
     gene            3176..3643
                     /locus_tag="VN496_05575"
     CDS             3176..3643
                     /locus_tag="VN496_05575"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXF16451.1"
                     /translation="MKQLLSLVSFCCALAGVMSAGMARAEDCGGPVTADEALAAEDAR
                     YAAQMGDDFGALQKLIGNDLVYIHSSAVVDNKASYIESMKSGTVKYRVMRRSDVTVRT
                     YGCVGIISGLGNFDVTVKGQDMAVEIRFHSIWAKRDRGLEFISWEATRTPQKQ"
     gene            3788..5233
                     /gene="mgtE"
                     /locus_tag="VN496_05580"
     CDS             3788..5233
                     /gene="mgtE"
                     /locus_tag="VN496_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXF16452.1"
                     /translation="MAKVVNKRKRTESVQEALEQVRELLHKHRIVESLVHEQQMPRHE
                     LVESLVHKQNLAELQRRLDKLHPADVAYILEALPLEERLVIWDLVKAERDGEILLEVS
                     DAVRETLIENMDAHELVAAAEQLDTDEIADLVPDLPPDVVDDVLHSLSIEEREQLRAA
                     MSYPEDAVGALMDFDMVSVRDDVSLEVVLRYLRRMDELPDHTDQVFVVDRDDVIKGVL
                     PINRMIVTDPDVLVGSVMNTEFITLSPDDKAQQAAQSFERYNLVSAPVVDHANRLVGR
                     VTVDVVVDFIRQRNEAEQLSHAGLLEEEDIFASVWKSAKNRWLWLAVNLGTAFFASRV
                     IGAFEGTIERLVALATLMPIVAGIAGNSGNQTTTLIIRSLALGQVTPENARRLIGKEV
                     AISVLNGLVWGGVAGMFAYMLYRSVPLGLVMMSAMLLNLLVGASVGMLVPLLMQRMGR
                     DPAIGSSVLLTFTTDSMGFFIFLGLATVFLI"
     gene            5348..6325
                     /gene="msrP"
                     /locus_tag="VN496_05585"
     CDS             5348..6325
                     /gene="msrP"
                     /locus_tag="VN496_05585"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HXF16453.1"
                     /translation="MLIRPPSDIKPSEITDHASFLGRRDFLRAATGLGVAGGLYGAGL
                     WMPGDAQAATKLAGVRKSTYSISETLTPLDAITSYNNYYEFGTDKADPAANAGKLKTR
                     PWQVGIEGEVKKPRVYDIEELQKLAPLEERVYRMRCVEGWSMVIPWVGFPLAELIKRV
                     EPTGNAKFVEFVTLLDPAQMPGQSVRVLQWPYVEGLRMDEAMHPLTLLTLGLYGEMLP
                     NQNGAPVRVVVPWKYGFKSAKAIVKIRFTERQPKTSWELAGPSEYGFYSNVNPHVDHP
                     RWSQAKERRIEKTSVLESLFEKKRDTLMFNGYGDQVAQLYAGMDLKKNF"
     gene            6357..6995
                     /locus_tag="VN496_05590"
     CDS             6357..6995
                     /locus_tag="VN496_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HXF16454.1"
                     /translation="MQPKPSHIISIKTALFVGCLIPLGHLFWQGFRNHLGANPVEYIT
                     HSTGWWALTFILITLSVTPLRRLASMPWLLRFRRLLGLFAFFYASLHFVTYIWLDQFC
                     NWRDIVKDIGKRPFILLGFTAFVLLIPLAMTSTNAMVKRLGAKRWQALHRSIYVLSAV
                     AVAHFWWLVKKDITEPFMFAVVLALLLIVRLLYLLKQQRSASPAGRTRSQAA"
BASE COUNT         1474 a         1913 c         2142 g         1562 t
ORIGIN      
        1 gccgcgcgca acgtcgcttc tctgtatggc cgctttccgg tggcttccct ccaggtcgtg
       61 gtgtcaccta cgccacgtgg ccggggtccg gtaccgtggg cctatgtttc gcgcggcggt
      121 ggcccggccg tgcatttgtt catcaatccg gcgcgcacgc ctgcagagtt cgagcgcgat
      181 tggagcctga cccacgaaat ggcgcacttg ttcctgccct acgtggatgc gcgtgatgcg
      241 tggttattcg agggtctgcc gacttacttg cagaacgtgt tgatggcgcg tggcggtgcg
      301 atcagcgttg aagaggcgtg gcggcgcatg cacgccgggt tcgagcgggg ggcacgtacg
      361 gcgcctgatg tcagcttcgc gcgcgcgaat gagcgcatcg gtcagggcaa catctattta
      421 cgggtttact ggggcggcgc ggcaatgatg ctcgccgccg acctgcagtt gcgggctcag
      481 acgggtggca agcagtcact cgctaccgcc ctggagcaat tgtcccgctg ttgcctcgga
      541 gacaatcggc gctggggtgc gcaagagatc attgaacggc tcgatcgcct ttccgggaca
      601 acagtattca cggatattgt gcgcgctcaa tttgctgcca acggatatcc ggactatgcg
      661 gacatcttga gccgcgccgg cgtaaaagtg gagggtgatc atgtggaatt ggacgcttca
      721 gcgccgctcg cggcggagcg cgaagcactg atgcacgccg cgcactaaac cagaatcgtg
      781 cctccggccg attcgattat catgaccggc tttcgccgac tgccttatga accctgaaga
      841 actgtgtttt ttgccggctc acgaactggc gcacctgata cgttcccgtg aattgtcggc
      901 ggcagaagtc atggacgcgc atatcgctca aatcgagcgg gtgaacccgg ccgtcaatgc
      961 aattgtgact ttcctgcccg agcgggcgca agaaagtgcg cgagccgtgg atgcggcgct
     1021 gaagcgtggc caagagcccg gtccgttggc cggcctgcca gtcgcgcaca aggatttgac
     1081 gccgaccaaa ggcatacgga ctacatttgg ttcgccgatc ttcaaggact tcgtgccgga
     1141 tgcggatgcg ctgattgtcg aacggcagag agcggccgga gctattacgg tggggaaaac
     1201 caatacgcct gaattcgggg ccggctcgca gacgttcaat ccggtgtttg gcccgacgcg
     1261 caatccttac gatcttggca agacatgtgg cggtagcagt ggcggggcag ctgcagccct
     1321 ggccagcggc atgctgccac tagccgatgg ctcggatttg ggtggcagcc tgcgcaatcc
     1381 cgccagtttt tgcaacgtgg ttgggctgcg tccctcgtcc ggtcgcgttc cgcactggcc
     1441 gagcgtttca gcgtggttca gcctcgcggt cgtcggaccg atggcgcgca ctgttcagga
     1501 tatcgcgttg ttcatggcat ccattgccgg gccggacgcg cgctcgccaa tttccttgca
     1561 ggatcccgca gccctgtttt cgcgcccgct cgaacgcgac ttcaaggatg tgcgaattgc
     1621 ctggagtacc gatctgggcg ggctgccgat ggaacctgag gtcatccgcg tcatgcaatc
     1681 ccagcgccag acctttgcag atctgggttg catcgtcgaa gaggcaactc cggatttgcg
     1741 cgatgcgcat gaaatcttca gcgtcttgcg cgcctggaac ttttaccttt cctacggcga
     1801 gctgttcaaa accaagcgtg ccttgatgaa agacacggtg atctggaata tcgagcaagg
     1861 cgcgcgcttg accggtcccg aagtcggcac tgctgaagtc aagcgcacgg aattatttca
     1921 ccgcatgcgc cagttcatga aaaaatatga ttttctggtt gcaccggtgg cgcaagttct
     1981 gccgttcgcc gtggacattc cttatcccaa ccggatcggg gacgtggcgc tcgacactta
     2041 tatcgactgg atgcgctcgt gttactacgt taccgtgacg ggcgcgcccg cgctttcggt
     2101 tccttgcggg ttcagcagca gtggactgcc ggtcgggctg cagatcgtcg ggcgttatca
     2161 ggatgatttt ggagtactgc aattaggcgc tgccttcgag caggccacag gctgctggaa
     2221 gcggcggccc gcgatcggac agaacaaccc ggagcttcgg acgctaaacc atgcactcaa
     2281 gatttgaccc gctttggcgg ttcacgaccc tcctcgtgat tgcggccctc gttattcctg
     2341 ccaaagctca gacaacgccc aaaccggctc cttcggcgcg caaggatagg gttttcctga
     2401 aggacattga aggtatctgg atcaacgaag cctaccttgg cgcgctggct gcgctgaaaa
     2461 gtccgcacgc ggcagcaaag aagacgcccc cgcttgtcat tgccatccgg cgcgaaggcg
     2521 ctgcttatcc gattgtgata acggattttc agaaggcttc gctgcaggcg gtgcttgata
     2581 tcgagcccga tggcaagccg aacgcctatc ggctggtcgt cgctgcggat gacaagccga
     2641 cgtcgagcaa cgaagtcaaa tacattcgtt tcgaagccgt gaagaacggt cagggaaaat
     2701 tcgaccggct gcatgttgcc gagccggact tcatgaaagg caggtgggcg gattacgttc
     2761 ctattgccgg tgaattgagc ccgcagctga accggtttgt cattgccggg aagtatcgcg
     2821 acgacaaggg acaaccgtgg gaattcacgg aatcggggga tgcaacatgg ccacagcaga
     2881 agttcaatta cgagttatcg ctaaacgacc cgggtgccag ctgcgattat ctgcaaagcg
     2941 agagcactgc agcagagccg cagaaaaaca gtgtcgagca ggataaaaag cgctacggtt
     3001 atgcttggaa agcgggcaag ctttccatct tccaggcacg cctggcaggc aagaaagtag
     3061 tttgcgagag caaaccactg gccgtgctga caccccacta gggtcgaaga cgaatgctgt
     3121 gccgaatgag caggaaatcg ttccggttcg ataaaaaatc caaagggagg aaaccatgaa
     3181 acagcttttg agtctggtat ctttttgttg tgctttagcg ggtgtaatga gtgccggaat
     3241 ggcacgcgcg gaggactgcg gtgggccggt aaccgcagat gaagcgctcg cagcggaaga
     3301 cgcccgctat gcggcgcaga tgggtgacga tttcggtgcc ttgcaaaaat tgatcggcaa
     3361 tgacctggtc tacattcact cgtcggcggt ggtggacaac aaggccagct acatcgaatc
     3421 catgaaatcc ggcacggtaa aatatcgggt catgcggcgc agcgatgtga cagtccgcac
     3481 ttatggctgt gtcgggatta tctccgggct cggcaatttc gatgtgacag ttaaagggca
     3541 ggacatggcc gtggaaattc gtttccacag tatctgggcc aagcgcgacc gcggcctcga
     3601 attcatttcg tgggaagcga cccgtacacc ccagaagcag tagcagtcct caagcgcgtt
     3661 gaaattcagc gcgcatccat tcggcggggc ttggacgaac aagccccgct tctgttttcg
     3721 gcgtctcgta atcaagtggt gatcaacatg cagtatcctt tgacgatgtt tgatccaatt
     3781 cctggtgatg gccaaggtcg ttaacaagag aaagcgcacg gagagcgtgc aggaagccct
     3841 ggagcaggtc agggaactgc tgcataagca ccgcatcgtc gaaagcctcg tccacgagca
     3901 gcagatgccg cgtcacgagc tggtcgagtc gctggtccac aagcaaaatc tggcggagct
     3961 gcaacgaagg ctcgacaagc ttcatccagc cgacgtcgcc tacattctcg aagcattgcc
     4021 ccttgaagag cgcctggtca tttgggattt ggtcaaggcg gagcgcgacg gggaaattct
     4081 gcttgaagtg tccgatgcag tgcgcgaaac actcatcgag aacatggacg cgcatgagct
     4141 ggtggctgct gccgagcagc tcgatacgga cgagattgca gatctcgttc cggacctgcc
     4201 gccggatgta gtcgatgatg tattgcattc gttgtccatc gaagagcgcg agcagttacg
     4261 tgccgcgatg tcttaccctg aagacgcggt cggtgcgttg atggactttg acatggtttc
     4321 ggtccgggac gatgtgagtc ttgaagtggt cctgcgctat ttgcgtcgca tggatgaatt
     4381 gcccgaccat accgaccagg tgtttgtggt ggatcgggac gatgtaatca agggcgtttt
     4441 gccaatcaat cgcatgatcg tgacggatcc ggacgtgctg gtgggctccg tgatgaatac
     4501 tgagttcatt accctgtcgc cagacgacaa ggcgcagcaa gcagcgcaat ccttcgagcg
     4561 ctataacctc gtctccgctc cggttgtcga tcacgccaac cggctggtcg ggcgcgtcac
     4621 cgtggacgtc gtcgtggatt tcattcgcca gcgcaatgaa gcagagcaac tcagccatgc
     4681 cggcttgctc gaggaagaag atatttttgc ttcggtgtgg aaaagtgcga aaaaccgctg
     4741 gttgtggctg gccgtaaatc tcggcacggc tttttttgct tcacgcgtca tcggtgcgtt
     4801 cgaaggaaca atcgaaaggc tggtggcatt ggccaccctg atgcccattg tcgcaggcat
     4861 tgcgggcaac tccggcaacc aaaccaccac attgattatt cgttcgctgg cgctcggaca
     4921 ggtgacgccg gaaaacgctc gtcgcttgat tggcaaggaa gtggcaatca gcgtattgaa
     4981 cggtttggtt tggggaggcg tggcagggat gtttgcctat atgctctatc gcagcgtgcc
     5041 gctcggtctg gtcatgatga gcgcgatgct gctcaatttg ctggtggggg cctccgttgg
     5101 catgttggtg cccctgctca tgcagcggat gggtcgggat ccagccattg gttccagcgt
     5161 gttgctgacc ttcaccaccg atagcatggg ttttttcatt ttccttggcc ttgccactgt
     5221 gtttttgatc tgacacatcc gttttttcgg ccggctcgta tatcaggcct gcaacgccaa
     5281 acatatcgga acttgcgctg tcccattgca tgcgaatacg ggactggccg gatacatagg
     5341 agcaaccatg ctgattcgac ctccgtctga catcaaacct tccgaaatca ctgaccatgc
     5401 atcctttctc ggtcgacgcg atttcctgcg tgcggcaaca gggttgggcg tggcgggggg
     5461 cctgtacggc gcgggcctgt ggatgcccgg agacgcacaa gcggctacca aactcgctgg
     5521 ggtcagaaaa agtacgtata gcatcagcga aacgctgacg ccgctcgacg ccatcacgag
     5581 ctacaacaat tactatgaat ttggcaccga taaagcagac cctgctgcca acgctggcaa
     5641 actcaaaacc aggccgtggc aagtcgggat tgaaggagag gtcaagaagc cacgggtgta
     5701 cgacatcgag gagttacaga agctcgcgcc gctggaagag cgggtctacc gcatgcgctg
     5761 cgtcgaaggc tggtccatgg tgatcccctg ggtcgggttt ccgctggcag aactgatcaa
     5821 gcgggtggag ccaaccggga atgcgaagtt tgtcgaattc gtgacgctgc tcgatcccgc
     5881 tcaaatgccg ggacaaagcg ttcgtgtgct gcaatggccc tatgtggagg gcctgcgcat
     5941 ggacgaagcc atgcatccgc tgacgctact gacgctggga ctttatggag aaatgctgcc
     6001 aaaccagaac ggcgccccgg tgcgcgttgt cgttccctgg aaatatggct tcaagagcgc
     6061 caaagccatc gtcaaaatcc gttttaccga acggcaaccg aagacatcct gggaattggc
     6121 cggtccgagc gagtatggtt tttattccaa cgtcaatccg catgtcgacc atccgcgctg
     6181 gagccaggcg aaggaacgtc gtattgaaaa aacatcggtc ctggagtccc tgttcgagaa
     6241 gaaacgtgac accttgatgt tcaacggtta cggcgatcag gtcgcgcaac tctatgccgg
     6301 aatggatctg aagaaaaact tctgaatcaa ccgccggtga gtgcttcttt tgatcaatgc
     6361 aacctaaacc atcgcacatc atctccatca agacggccct gttcgtaggt tgccttatcc
     6421 cgctggggca tttgttctgg cagggttttc gcaatcatct tggtgccaac ccggtggaat
     6481 acatcacgca ctccaccggc tggtgggcgc tgacattcat tttgattacg ctgagcgtaa
     6541 ccccattgag aagattggcg agcatgccgt ggctactgcg ctttcggcgc ctgctcgggc
     6601 tatttgcatt tttctatgcc agcctgcact tcgtgactta tatctggctg gaccagttct
     6661 gcaactggag agatatcgtt aaggacattg gcaagcggcc cttcatcctg ttgggattta
     6721 ctgcatttgt gctgctcatt cctttggcga tgacgtcgac gaacgcaatg gtgaagcgcc
     6781 ttggcgccaa acgctggcaa gcacttcatc gatccatcta tgtgctgtcg gccgtggccg
     6841 tggctcattt ttggtggctg gtaaagaaag atattaccga accgtttatg tttgctgtgg
     6901 tgctcgcgct gctattgatc gtcaggctcc tgtatcttct aaaacagcag cggtcagcat
     6961 cgcctgcggg gcgaacgcgc tctcaagcgg cgtgatcggt ggttctaaaa tcgcactcaa
     7021 ggtgtcaatc gcatgctggt cggtgtgccg aaggagatca aagatcatga gtaccgtgtc
     7081 gggttaacgc c
//
LOCUS       DATMZQ010000172         3806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1863029, whole genome shotgun sequence.
ACCESSION   DATMZQ010000172 DATMZQ010000000
VERSION     DATMZQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863029"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VN496_05595"
     CDS             complement(<1..437)
                     /locus_tag="VN496_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HXF16455.1"
                     /translation="MKQIALTLAPVPAPALENFYPGRNAELVTLLGNLVAGRNVERSI
                     YLWGDPGSGRSHLLHATIGALKASGLVCICVSRGDSMPASSQLRALAVDDVETLNAGN
                     QRAFFNLYNELRERSGIVLAAGNAPPAQLKLRPELMTRLAWGL"
     gene            685..1728
                     /gene="purM"
                     /locus_tag="VN496_05600"
     CDS             685..1728
                     /gene="purM"
                     /locus_tag="VN496_05600"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXF16456.1"
                     /translation="MKTPAFNSLSYRDAGVDIDAGDELVENIKPFARRTLREGVLAGI
                     GGFGALFEVPKKYHEPVLVSGTDGVGTKLKLAFHLNRHDTVGIDLVAMSVNDILVQGA
                     EPLFFLDYFACGKLDVATATQVVKGIAFGCEQAGCALIGGETAEMPGMYPTGEYDLAG
                     FAVGAVEKTKIIDGKDIAAGDTVLGLASSGAHSNGYSLIRRIIDGSGVRLSSDFGDGT
                     LGEALLAPTRIYVKAVLAAMAKVRIKGLAHITGGGLVENVPRVLPETVCAELQRDAWP
                     RPALFDWLQKSGKLPDAEMHRVFNCGIGMAMIVAQEDAANAMRALEREGESVFSIGKI
                     RDRSPGEAQTLIV"
     gene            1730..2368
                     /gene="purN"
                     /locus_tag="VN496_05605"
     CDS             1730..2368
                     /gene="purN"
                     /locus_tag="VN496_05605"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXF16457.1"
                     /translation="MGTKRIVILISGRGSNMKALLDARLNATVVAVISNIPTAMGLQI
                     AKAAGVDTKIVNHQDHGDRASFDHCLAETIAAYSPDLIVLAGFMRILSQEFVVRFSGK
                     IMNIHPSLLPAFPGLNTHQRAINEGVRLHGCTVHFVTSQLDHGPIIIQAAVPVLPDDD
                     EQTLASRVLEQEHAIYAQAIRWFVDDKLAIEGNRVKVAADCRYLGALISPEP"
     gene            2365..3060
                     /locus_tag="VN496_05610"
     CDS             2365..3060
                     /locus_tag="VN496_05610"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXF16458.1"
                     /translation="MTIRYALLMVMLVCAPCFAAQPPHRVQMEFSVNSGSMHLGEGRD
                     VLEHDGKRYSVISESKTVGLASFFYKMNVRRESRGLITNQGLKPLHFEEDRSRKGRRT
                     VDFDWDAKLVKLTDGDNVETVPLPDNTFDQTTFTYAFAFRPPGEEIAPVHLTDGRKLS
                     DYKYKIVGKEKLKTSLGELETVHFQKMQEADDKRGFEVWLAIGHYYLPVQIRFVEKDG
                     TVLDSTVTAISYP"
     gene            3064..>3806
                     /locus_tag="VN496_05615"
     CDS             3064..>3806
                     /locus_tag="VN496_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16459.1"
                     /translation="MQPTAAIMQAATVAVDLALQLLNPADSVLRNFFAANRNLGQRDR
                     AFIAEIVFSVLRHKRLLEQLIGDPSPRRLVCAALIKLQGYSLGQLDAFLPSADRDWTL
                     GLKTAEIESLPPAVRLSLPDWLYDRLVAETSRDDVLAFGRAMLNPAPLDLRVNTLLAE
                     REQVLRDLRASGLDALPTRYSPVGIRIKGKPAINRHVLFQNGTIEVQDEGSQLLGYLV
                     APKRREMVVDFCAGAGGKTLLMGAMMQSQG"
BASE COUNT          897 a         1011 c         1069 g          829 t
ORIGIN      
        1 atcagccccc aagccaatcg cgtcattagt tccggacgca acttcagttg cgctggcgga
       61 gcgttgccag cagccaagac gatgccggaa cgttcccgca gctcattgta caaattgaag
      121 aacgcgcgtt gattaccggc attcagcgtt tcgacatcgt ccaccgccag tgcgcgcaat
      181 tgcgacgatg caggcatcga atcgccacgg gacacgcaaa tacaaaccag gccggacgcc
      241 ttcagcgccc cgatagtggc atgcaacaaa tgcgaccgtc cgctacccgg gtcgccccac
      301 aaatagatcg aacgctcgac atttcgtcct gcgacaagat tgccaagcag ggtcaccagt
      361 tcggcattgc gcccgggata aaaattttcg agggccggtg cgggaaccgg cgcaagtgtc
      421 agtgcgattt gtttcatcag gacagaattc cacagcccga acaaaactgc agcaccgatt
      481 cgaaaagcgg caccgagggc gcacctacca ggcgggtggg caccggtggc gatgcactat
      541 gagaccatcc ctcccccgcg ttcagatacc aagccagatg ctcaggaaat acggtagaca
      601 tttggagtaa aattcgccgc gcaataccat acgaccggcc tcaatccgga tctcactcta
      661 tcccgaagaa ctcggcaact caacttgaag actccggctt tcaactcgct atcgtatcgt
      721 gacgcgggcg tcgatatcga tgctggcgac gaactagtcg aaaatataaa gcctttcgcc
      781 aggcgcacac ttcgcgaagg tgttctggca ggaatcggcg gatttggcgc gctgttcgaa
      841 gttccgaaaa aataccacga accggtgctg gtgtccggca ccgacggcgt cggtaccaaa
      901 ctcaaactgg catttcacct caaccggcat gatacggttg gcattgacct ggtcgcgatg
      961 agcgtaaacg atattttggt gcaaggtgcc gaaccgctgt tctttttgga ttacttcgcc
     1021 tgcggaaaac ttgatgttgc aactgccacg caggtggtca aaggtattgc tttcggttgc
     1081 gagcaggctg gttgcgccct catcggaggg gaaactgcgg aaatgccggg catgtatcca
     1141 accggagaat atgacctggc aggattcgcc gttggcgccg tggagaagac gaaaatcata
     1201 gatgggaagg atattgctgc gggcgacacg gtactcggcc tggcaagcag cggagctcat
     1261 tcgaacggct attcacttat acgcaggatc atcgatggct ctggcgtacg cctgtcatcc
     1321 gactttggcg atggaacgtt gggagaggcg ttgctcgcac caacgcgcat ctatgtgaag
     1381 gcggtgcttg ccgcgatggc taaggtgcgt atcaagggac ttgcgcatat aactggcggc
     1441 ggtctggtag aaaatgtgcc ccgtgtgttg ccggagacag tgtgcgcgga actccaaaga
     1501 gacgcatggc ctcggccagc gttgttcgac tggcttcaga agagcggcaa actacccgat
     1561 gctgaaatgc atcgcgtgtt caattgtggc atcggcatgg cgatgattgt tgcgcaagag
     1621 gatgctgcca acgccatgcg ggcgctcgag cgcgaaggag aatctgtttt cagcattggg
     1681 aaaattcgag accggagtcc tggcgaggca cagacactga tagtctgaaa tggggacgaa
     1741 gcgcattgtc attctgatct ctggccgcgg cagcaacatg aaagcgcttc ttgacgccag
     1801 gctgaatgca accgttgtag cggtaattag caatataccc actgcgatgg gattgcagat
     1861 tgcgaaagcc gcaggcgtag atacaaagat cgtcaaccat caggaccatg gcgaccgtgc
     1921 aagtttcgac cattgccttg cagagactat cgcggcctat agtccagacc ttatcgtgtt
     1981 ggcgggattc atgcgaatac tgtcgcaaga gtttgtcgtt cgctttagtg gcaaaatcat
     2041 gaacatccat ccttcgttac tgccagcgtt tcccggcctc aatacccacc aacgtgcgat
     2101 caatgaaggc gtcagacttc atggttgcac ggttcatttc gttacgtctc aacttgacca
     2161 cgggccgatt atcattcaag ccgccgtgcc ggtacttcct gatgatgacg aacaaacgct
     2221 ggcgtcgcgc gtccttgagc aggagcatgc catctacgcg caagccattc gctggttcgt
     2281 tgacgacaaa ctggcaatcg aaggaaacag ggtcaaagtc gcagccgatt gccgctactt
     2341 aggggcgctt atttctcctg aaccatgaca atacgctacg ctctgttgat ggtgatgttg
     2401 gtgtgcgccc cgtgttttgc tgcacaacct ccacaccggg tacaaatgga attttcggtc
     2461 aattcgggaa gcatgcatct tggcgagggg cgggatgtgc tcgaacatga tggcaagcga
     2521 tattccgtaa tcagcgaatc caagactgtt ggtttggctt ccttcttcta caaaatgaat
     2581 gttcgaaggg aaagccgcgg actaatcacc aatcaaggac tgaagccgct gcattttgag
     2641 gaagatcgtt cacgcaaagg ccggcgcact gtagatttcg attgggacgc aaaacttgtc
     2701 aagttgaccg atggcgacaa tgttgaaact gttccattgc cggataacac gttcgatcag
     2761 accaccttca cgtatgcgtt cgcattccgg ccccccggcg aagaaatcgc gccggtacat
     2821 ctgaccgacg gcaggaaact gtccgattac aagtacaaga ttgtcggcaa ggaaaaactc
     2881 aagacttcgc ttggtgaact ggagacagtt cactttcaga aaatgcagga agccgacgac
     2941 aagcggggat tcgaggtatg gctcgcgata ggtcactatt acttgcccgt gcaaattcgc
     3001 ttcgtcgaaa aagacggaac ggtacttgat tcgaccgtca ctgcgatttc ctacccctaa
     3061 caaatgcaac cgaccgctgc aataatgcaa gcggccacgg ttgccgtaga cctcgcattg
     3121 caattgctca atccggcgga ttcagtattg cgcaacttct ttgctgcaaa tcgcaatttg
     3181 ggtcagcgtg accgggcctt catcgccgaa attgtatttt cggtgctgcg tcacaaacga
     3241 ctgttggaac agttgatcgg ggatccgagt ccacgcaggc tcgtttgcgc cgccctcatc
     3301 aaactacagg gctacagcct tggacagctt gacgcttttc tgccaagcgc cgatcgcgat
     3361 tggacccttg ggttgaagac ggcagagatt gagtccctcc caccagcggt aaggcttagc
     3421 ctgcctgact ggctgtacga tcgtctggtg gcggagacat ctcgagacga tgttctcgct
     3481 tttggtcgtg ccatgctcaa tcccgcaccg ttggacctgc gggttaatac gctactggcc
     3541 gaacgtgaac aggtattgcg cgacttgcgg gcgagcgggc tcgacgcctt accgacgcgt
     3601 tattctccag tggggattcg gatcaaagga aagccggcca tcaatcgcca tgtcctgttc
     3661 caaaacggca ccatcgaggt tcaggacgag ggaagtcagt tactggggta tttggttgcc
     3721 ccaaaacgcc gtgagatggt ggtggacttc tgcgccggag cgggtggaaa gactttgttg
     3781 atgggcgcta tgatgcagtc ccaggg
//
LOCUS       DATMZQ010000173         3542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2898421, whole genome shotgun sequence.
ACCESSION   DATMZQ010000173 DATMZQ010000000
VERSION     DATMZQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2898421"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VN496_05620"
     CDS             <1..963
                     /locus_tag="VN496_05620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXF16460.1"
                     /translation="YVAKYSRGQESQADKLGAEYLSRVNYDPHNMVDVIKVLENQERF
                     AADQARAEGRAPRKGGSDYLASHPSNEQRLRDITQIANQYQAKASYGDEGHKRYLQMI
                     DGVSFGESAEQGVTRGRNFYHEPLGIALTAPAGWRFQNSRDALALVNGESNAAVIVKA
                     VPAQAGNSHEDIIRNAIKPNSGKMERRTFNGLPATHFSGTVKDENGQSQGVELTLVTG
                     PANTNYALIYLAKDQQSLRQAYPQIQQAESSFRAMTPADRDAARPWKLKSVPYPPGGF
                     AELAKHSPLANNAEQQLRLLNGVYGGGDVKPGDLVKVVEPADLA"
     gene            1083..2051
                     /locus_tag="VN496_05625"
     CDS             1083..2051
                     /locus_tag="VN496_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF16461.1"
                     /translation="MRIFTRLSLLVLLCAAPAANALDRVTFLTNWFAEAEHGGFYQAV
                     AEGIYQKHGLDVKIGMGGPQVNVYQLLLAEKADFVMGYDVATINAVEQNLPLITVAAN
                     FQSEPVGLIAHPDVQKIEDLKTRTLLIGQASETTYWPWLKAKYGFTEAQKRPYAYSVQ
                     QFLVDKNIAQQGYITSEPYAIEKGGVTPKIFHLAKYGYPPYAQTIVTLTKTAKQRGDL
                     VKRFIEASALGWKSYLKNPAPGNALIKRDNPQMDDEQLAFSLAKLKEYGIVDGGDAKK
                     LGLFTMTDARWKQTFEFMSNAGLVKPDVDVHKAYTLDFVKQVKVLP"
     gene            2062..>3542
                     /locus_tag="VN496_05630"
     CDS             2062..>3542
                     /locus_tag="VN496_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXF16462.1"
                     /translation="MLQQAESYEAVYRGFRWTIPEYFNIGVDICDKWCADPARLALIH
                     EKRDGSSSHYTFADIRQLSNQAANLFVRRGLRPGDRVGILLPQEPETAIAHVAAYKAG
                     AIVVPLFTLFGLDALRFRLQDAGVGILLADARSLDKIAQIRADLPDLHTVLCIDGAVA
                     GSEHFHAALGSMSREFQPVRTKADDPALIIYTSGTTGPPKGALHAHRTLLGHLPGVEM
                     SHNFLGMAGDLMWTPADWAWIGGLIDVLLPAWHHGIPVLAHRFDKFEPEAAFALMAKH
                     AVRNVFLPPTALKMMRGVPDAHVRWPLNLRSLASGGETLGAELLQWGRQTFGLTINEF
                     YGQTECNMVLSSCEALFPSKPGSIGKAAPGHAVEIIDEAGKILPPDTIGAIAIRRPDP
                     VMFLGYWNNPRATQEKFVRDWLVTGDTGAKDADGYVRFVARNDDVITSAGYRIGPGPI
                     EDCLLSHRAVRMAAVVGTPDVERTEIVTAFVVLQTGCVPSDQLT"
BASE COUNT          800 a         1020 c         1041 g          681 t
ORIGIN      
        1 tatgtagcaa aatacagccg tggtcaagag tcgcaggcgg acaagctggg cgccgaatat
       61 ctctcccgcg tcaattacga tccgcacaac atggtcgatg tcatcaaagt gctcgagaac
      121 caggagcgct tcgccgcaga ccaggcgcgc gccgaaggcc gcgcaccccg gaaaggcggc
      181 tcggattatc ttgcatcgca tccgagcaac gagcagcgac tgcgcgacat aacgcagatt
      241 gcaaatcagt accaggctaa ggccagctat ggcgacgaag gccacaagcg ctatctgcag
      301 atgatcgacg gcgtgtcatt cggcgaaagt gcggaacagg gtgtaacgcg gggacgcaat
      361 ttttatcacg agccattggg catcgccctg acggcgccag cggggtggag atttcagaac
      421 tcgcgggatg cgctcgcgct tgtgaacggc gagagtaacg cagcggtgat cgttaaggca
      481 gtaccggcgc aagccggcaa tagccacgag gacatcatcc gcaatgcgat caagccaaat
      541 tcgggcaaga tggaacgccg cactttcaac gggcttccgg ccacgcattt ttccggtacg
      601 gtcaaagacg aaaatggcca gtcacagggg gtcgaattga ctctggtcac cggtccggcg
      661 aacacaaatt acgccctcat ctatctggcc aaggatcagc agtcgcttcg gcaggcgtac
      721 ccgcagatac agcaggctga atcgtcgttt cgcgcgatga caccggcaga tcgcgatgcg
      781 gcgcgtccat ggaaactgaa gagcgtcccg taccctcctg gcggctttgc cgaactggcg
      841 aaacactccc ccttggccaa taacgcggag cagcagttac gcctgctgaa cggggtttac
      901 ggtggaggtg atgtaaagcc gggagacctg gtgaaagtag tggaaccagc cgacttggcc
      961 tgatcatagc ttgcatgtcg aatcatgctg tccgcaaggg cgagcaggtt cctgcaccag
     1021 gaatcccagc aaccctatga cggggtaaac tctgcgattt ctcgatactg gaatccagac
     1081 ccatgcggat cttcacccgg ctttcactgc tcgtattgct gtgcgcggcc cccgcggcga
     1141 atgcgctaga ccgagtcacc tttcttacca actggttcgc cgaagcagaa catggcggtt
     1201 tctatcaggc ggtagcagaa ggcatctacc agaagcacgg cctggacgta aagattggca
     1261 tgggcgggcc ccaggtgaac gtctatcagt tgttgcttgc cgagaaagcg gatttcgtaa
     1321 tgggctacga cgtcgcgacc atcaacgccg tcgaacagaa tttgccgctg atcacggtag
     1381 ctgcaaactt tcagagcgaa ccggtcggat tgattgcgca cccggatgtg caaaaaatcg
     1441 aagacctgaa aacccgcacg ctgttgatcg gacaggccag cgaaaccact tactggccgt
     1501 ggttgaaagc gaaatatggt ttcaccgaag cgcaaaagcg gccgtacgcg tactcggtgc
     1561 agcaatttct ggtggacaaa aatatcgcgc aacagggtta catcacttcg gaaccttacg
     1621 ccatcgaaaa aggcggcgtg accccgaaaa ttttccatct cgccaaatac ggctacccgc
     1681 cgtacgccca gaccatcgtc acgttgacca agaccgcaaa gcagcggggg gatctggtga
     1741 agcgctttat agaggcttcc gcgctcggct ggaaaagtta tctgaagaat ccggcaccgg
     1801 gcaatgcact catcaaacgc gacaatccgc agatggatga tgagcaactc gccttcagtc
     1861 ttgccaagct aaaagaatat ggcattgtgg atggtggcga tgcgaagaag ctcggcctgt
     1921 tcaccatgac ggatgcgcgc tggaagcaga catttgaatt catgtcgaac gcaggcctgg
     1981 tcaagcccga cgtggatgtt cataaagcct acacactgga tttcgtaaaa caggtcaaag
     2041 ttctgccctg agagtccgtc agtgctgcaa caggccgaga gttatgaagc cgtttaccgc
     2101 ggcttccgct ggaccattcc tgaatatttc aacatcggcg tcgatatttg cgacaagtgg
     2161 tgcgccgatc ccgcgcgcct tgcactgatc cacgaaaagc gcgacggttc ttcgtcgcac
     2221 tacacgttcg cggatatccg gcagctctcc aaccaggcag cgaacttgtt tgtccgccgc
     2281 ggattgcgcc cgggagatcg ggtcggcatc ctcctgccgc aggaaccgga aaccgcaatc
     2341 gcgcacgtcg ccgcttacaa ggcaggtgcg attgtcgtgc ctctgtttac gctgtttggc
     2401 ctggatgcgc tgcgtttccg cctgcaggat gccggcgtag gaattttgtt ggccgatgcg
     2461 cgcagcctgg acaagatcgc acagatccgc gccgacctgc cggacttgca cacggttcta
     2521 tgcattgatg gcgcagtcgc gggcagcgaa cactttcacg ccgcactcgg ctccatgagc
     2581 cgggaattcc agccggtgcg caccaaggcc gacgatcccg cgctgatcat ctacacctcg
     2641 ggaaccacgg gcccgccaaa aggggcttta cacgcacatc gcacattgct cggacatctg
     2701 ccgggcgtgg aaatgtcgca caattttctg ggcatggccg gcgatctgat gtggacaccg
     2761 gcggactggg catggatcgg aggtctgatc gacgtgctgc tgccagcctg gcatcacggc
     2821 ataccggtgc tcgcgcatcg cttcgacaaa ttcgagccgg aagccgcttt tgctttgatg
     2881 gcgaaacacg cggtgcgcaa tgtatttttg ccgccgacgg cgctcaagat gatgcgtggc
     2941 gtgccggacg cgcatgtgcg ctggccattg aatctcaggt cgcttgcgag cgggggagaa
     3001 acgctgggtg ccgaactgct gcaatgggga cgccaaacct tcggcctcac gatcaatgag
     3061 ttctatggtc agaccgaatg caacatggtg ctgtcttcct gcgaagcgct gttcccctcg
     3121 aaacctggct cgatcggcaa ggccgcaccg ggacacgcag ttgaaattat cgatgaagcg
     3181 ggaaaaatat tgccgccgga caccatagga gccattgcca tccgccgtcc cgatcctgtg
     3241 atgttcctcg gctattggaa caatccccgc gccacgcagg aaaaattcgt cagggactgg
     3301 ctggtcaccg gggatactgg tgcaaaggac gccgatggct atgtgcgctt cgtcgcacgc
     3361 aacgacgacg tcatcaccag cgcggggtac cgcatcggtc cgggccccat cgaagattgc
     3421 ctgctcagcc accgtgccgt gcgcatggcc gcggtggtcg gtacaccgga tgtcgaacgc
     3481 accgaaatcg ttacggcatt cgtcgtgctg cagacgggtt gcgtgccgag tgaccaattg
     3541 ac
//
LOCUS       DATMZQ010000174         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3244588, whole genome shotgun sequence.
ACCESSION   DATMZQ010000174 DATMZQ010000000
VERSION     DATMZQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244588"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VN496_05635"
     CDS             <1..188
                     /locus_tag="VN496_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16463.1"
                     /translation="MTETRTSRRGTMALSERRINITTITKINAANAPANGAGNTSGVE
                     SSPFADALSAIDMGSSY"
     gene            complement(182..1144)
                     /locus_tag="VN496_05640"
     CDS             complement(182..1144)
                     /locus_tag="VN496_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXF16464.1"
                     /translation="MRVFLRLAAVLFAILQVWPAAAQNYPSRPVKFIVPFGAGGPADV
                     FARVLAQHLSEETKQSFIVEDRPGAGSIIGTDAVAKSAPDGYTLLVMSNTHTTNESLL
                     PNKPFQLMRDFVPVATINYSDLLMVVHPSVPAKTVKEFIELAKKDPGKLNYASSGPGT
                     PYHMAGELFKAMSQTDIVHVPHKASGDARNSVVSGTVQMMFDAVTTMSELAKSGQVRA
                     LGTTGLTRSQLTPDLPTIAETVSGYEATIWLGVMAPKGTPKEIINFLNTNINKVINRP
                     DVKEAWLKQGAIPLIKTPEEFEAFPVKDIEKWANVVKVSGAKIQ"
     gene            complement(1196..1531)
                     /locus_tag="VN496_05645"
     CDS             complement(1196..1531)
                     /locus_tag="VN496_05645"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXF16465.1"
                     /translation="MPIYITQGRYTREAIKGMIVKPEDRADAVARILSKIGGRLLGYY
                     LTFGEYDFLAIAEAPNDVQMAAVLLAAGSGGGVTGLKTTVAMTSVEAKGAFAAASDLA
                     PGFRSAGGT"
     gene            complement(1625..2056)
                     /locus_tag="VN496_05650"
     CDS             complement(1625..2056)
                     /locus_tag="VN496_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16466.1"
                     /translation="MSKHSAKTAGRSAKTTSRTAKKSAKSVRVTAKKTSARLPVGKVT
                     RPVRMIAPPIVPVKATKMIRTPSPPVQPVAMHELPIRSHKYQVGETVYYTSPTFGRAA
                     ATGSYIVVKLLPSEGDDYQYRIKSSGEAFERVAKESQLDRA"
     gene            2280..2486
                     /locus_tag="VN496_05655"
     CDS             2280..2486
                     /locus_tag="VN496_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16467.1"
                     /translation="MRVLIGIILGAIITVGGAYLYDSSHAVTNSDSATVAQRPMVNWD
                     VVGNKWNHLTARARAEWSRLASKT"
     gene            complement(2502..>2817)
                     /locus_tag="VN496_05660"
     CDS             complement(2502..>2817)
                     /locus_tag="VN496_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16468.1"
                     /translation="YGATNPTCLSGVSSDHGVGGKAKRFAMHYRVYSGPKGSPDVSPL
                     EKSQLLFKEFADLDDALSWARHLEGTGRIPLLIEGDDGTRMNRRAIIDALRVGVREQL
                     RD"
BASE COUNT          566 a          848 c          791 g          612 t
ORIGIN      
        1 cgatgacgga gaccagaaca agcaggcgcg gaacgatggc gctgtctgaa cggcgcatca
       61 acatcacgac catcaccaag atcaacgccg ccaacgcacc ggccaacgga gccgggaaca
      121 ccagcggcgt tgagagcagc ccgtttgcgg atgcgctatc ggcgatcgac atgggttcca
      181 gctattgaat cttcgctccc gagactttta ccacattcgc ccatttttcg atgtccttta
      241 cggggaaggc ttcgaattct tcaggcgtct tgatgagcgg tatcgccccc tgcttgagcc
      301 atgcttcctt cacatcgggc ctgttgatga ccttattgat gttggtgttc aggaaattga
      361 tgatctcttt aggcgtgccc tttggcgcca tcacgccgag ccagatcgtc gcctcgtagc
      421 cggacaccgt ttcagcaatc gtcgggaggt ccggggtcaa ctgcgagcgt gtaagacccg
      481 tcgttccgag tgcacgcacc tgccccgact tggcaagttc cgacatcgtg gtcacggcat
      541 cgaacatcat ctgcacggtt ccggaaacca cgctgttgcg cgcatcgccc gaagccttgt
      601 gcgggacgtg gacgatgtcc gtttggctca ttgccttaaa gagttcgccg gccatgtgat
      661 agggcgtgcc tggaccggac gatgcgtaat tgagcttgcc gggatccttc ttcgccaatt
      721 cgatgaactc cttgacggtt tttgccggaa cggacggatg aacgaccatg agcagatccg
      781 agtagttgat tgtcgccacc ggaacaaaat cacgcatcaa ctggaacggc ttgttgggca
      841 gcagagactc gttggtcgtg tgcgtgtttg acatcaccag cagcgtgtag ccatcgggcg
      901 ccgacttcgc gacggcatcg gtcccgataa tcgaacctgc gcccggacgg tcctcgacga
      961 tgaatgactg cttggtttcc tccgaaaggt gctgagcaag cacgcgggca aatacgtcgg
     1021 caggcccgcc cgctccgaaa ggaacaatga acttaacggg acggctcggg tagttctggg
     1081 ccgcagcagg ccacacctgc aagattgcaa atagcactgc ggccagacgc aaaaagacgc
     1141 gcataggtcc tcccctcaaa agtttgttcg ttgatgacgt cgtcgtttct tctcgtcatg
     1201 tcccgccagc ggatctaaac cctggcgcta aatcgctcgc cgcggcgaat gctcctttgg
     1261 cctcgaccga cgtcatcgca accgtcgttt tcaagccggt cacacccccg ccgctccccg
     1321 cagccaaaag cacggccgcc atctgaacat cgttgggcgc ttcagcaatg gccagaaaat
     1381 cgtattcgcc gaatgtcagg tagtagccca atagtcgccc accgattttc gacaagatgc
     1441 gcgcaacggc gtcggcccgg tcctcaggct ttacgatcat cccctttatg gcttcacggg
     1501 tgtaccgccc ctgcgtgatg tagattggca tccccggcct cctcttgcgc agcaaactgc
     1561 gacgtccggc gcatcatatg gagcggccac ggcaaaatag cgacaggagg cctcgcagcc
     1621 ggcgtcaggc gcggtcgagc tggctctctt tggccacgcg ttcgaacgct tcgcccgagc
     1681 ttttgatccg gtactgatag tcgtcaccct cggacggcaa gagcttgacc acaatataac
     1741 ttccggtcgc ggccgcacgt ccgaaggtcg gcgatgtgta atagacggtc tcgccgacct
     1801 ggtacttatg cgaacggatc ggcagttcgt gcatcgccac cggctggacc ggcggactcg
     1861 gcgtgcggat catttttgtc gcttttaccg ggacaatagg cggcgcaatc atacgcactg
     1921 gacgcgtgac cttgccgacc ggcagacgag ccgatgtctt ttttgccgtc acccgaacgc
     1981 tcttcgcgct cttttttgcg gtccggctcg tcgttttggc cgaccgcccg gcggtttttg
     2041 ctgaatgttt gctcataatt tcaatatagc agcaaatttt gtttgcgggc aaagtttttt
     2101 ggcgtggaag cttaatgttt tcaatgagtt ataacacaag aaagggctgt acaaggttaa
     2161 cggcgcgcga cccaaaatgc gcctccttgg ctgtcataat tgctcgccgg cccctgtaga
     2221 accagaggga actcgcggtc atcaggggcg tttcaggtcg ggaacgagga gttttcatca
     2281 tgcgtgtcct tatcggcatt atccttggcg ccataatcac cgttggcggc gcctatctct
     2341 atgactcgag ccatgcggtg acgaattccg acagcgcaac cgttgcacaa cgtcccatgg
     2401 tgaattggga tgtcgttggc aacaaatgga accatctcac ggcgcgcgcc cgcgccgaat
     2461 ggagccggct cgcttccaag acctgaccca ttgccggccg gctaatcgcg cagttgctca
     2521 cgcactccta cccgcaacgc atcgatgatc gcgcgacggt tcatgcgcgt gccgtcatcg
     2581 ccctcgatca ggagtggaat gcggcccgtt ccttcgagat ggcgggccca cgagagggcg
     2641 tcatcgagat ccgcgaactc cttgaacaac agctgcgatt tctcaagcgg cgacacatcg
     2701 ggcgagccct tggggccact gtagacacgg tagtgcatcg caaacctctt ggctttgcca
     2761 cccaccccat gatctgaact aacgcctgac aggcatgtgg ggttcgtcgc tccatat
//
LOCUS       DATMZQ010000175         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4904319, whole genome shotgun sequence.
ACCESSION   DATMZQ010000175 DATMZQ010000000
VERSION     DATMZQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4904319"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VN496_05665"
     CDS             <1..563
                     /locus_tag="VN496_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16469.1"
                     /translation="GSHLGESLVAAPVVLVTAGLLLRTARGRLIFMTLTAAGIVGFLL
                     WRSAGAHAAWFYPVPYMTVYLMLLWAFGSTLLPGRLPLITRIATLVHGEIPIEIVRYT
                     RSITWAWCVFFAAMATISASLFLFAPLRVWSIFNSLLNLPLVVLMFLGEYAWRLWRYP
                     HFSHASIATMLQAMRNFEFHRPSAGR"
     gene            568..1935
                     /locus_tag="VN496_05670"
     CDS             568..1935
                     /locus_tag="VN496_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXF16470.1"
                     /translation="MAAVAAITHADAADAVAYRGAQRISAGDFVAQVEQVARRLPERP
                     FLVNLCTDRCRFAVGLIAAMARGQVSLLPPNQTPDMLRRLQQHYGGLYVLSDSVQRPE
                     ILESMMYPETQIRTQRTQHFKVPAFPENQIAAIAFTSGSTGMPAPHRKSWGALTRGAT
                     AELERFGLHRGERAGLVSTVPAQHMYGLESTVLMPLRGGLVMHAGRPFYPADVAAALA
                     QIPGDRVLVTTPVHLRALVTAGTALPKLRLVICATAPLSPQLAALVEDRYEVPLQEVY
                     GFTEAGMVATRRTVHGAIWQCLNHVRLRRVGDAAASVRGGHIETEVACSDLIQPLGDH
                     DFILHGRNADLVNIAGKRTSLGHLDHQLNSIEGVHDGVFLMPDEHGEGITRLTAFVVA
                     PELGSDTVLRALRDRIDEVFLPRPLYFVAALPRNATGKLPREDLLRFANDCAAAAARK
                     KEEVG"
     gene            1932..>2183
                     /locus_tag="VN496_05675"
     CDS             1932..>2183
                     /locus_tag="VN496_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXF16471.1"
                     /translation="MSTVRLFFAILAMCIGMPGVTAPADQPARNWGVEMLMQQLSQVE
                     HSQARFVERKYLKALKAPLELSGTLSYSRPDRLEKRTLKP"
BASE COUNT          401 a          645 c          657 g          480 t
ORIGIN      
        1 ccggcagtca ccttggagag tcgcttgtgg cggcaccagt tgttctggtc acagcagggt
       61 tgttgctccg gacggcccgc ggtcgcctta ttttcatgac attgactgcg gccggcatcg
      121 tcgggttcct gctatggcgc agtgccggtg cgcatgctgc ctggttctac ccggtgccgt
      181 atatgactgt ctatctgatg ctgttatggg cgttcggatc tactctgctg ccgggacgcc
      241 tgccgctcat tacccggatt gcaactctgg tccatggcga aattccaatc gagatcgtgc
      301 ggtatacccg cagcataacc tgggcctggt gtgtcttctt cgccgccatg gcgacaatat
      361 ctgcatcgct gttcctgttt gccccgctgc gggtgtggtc gatattcaac tcgttgttga
      421 acttgccttt ggtggtcctg atgtttcttg gcgagtacgc ctggcggcta tggcgttacc
      481 ctcatttctc acatgcgtcg attgccacga tgctccaggc catgcgcaac ttcgaattcc
      541 accgaccttc ggctggtcgc taaacgcatg gcggcagtag cggccataac ccatgccgat
      601 gcggccgatg cggttgcgta ccgtggggca cagcgaatca gcgccgggga ttttgttgcg
      661 caggtcgagc aggtggcgcg gcggttgccc gagcgcccgt ttcttgtcaa tctttgtacc
      721 gaccgttgcc gatttgcagt cggcctgatc gcggcgatgg cacgcggaca agtgagcctg
      781 ttgccaccaa atcaaacacc ggacatgctg cggcgattgc aacagcatta cggcggtctc
      841 tacgtgctca gcgattcagt ccagcggccg gaaatcctgg aatcgatgat gtatccggaa
      901 acgcaaatac ggacacagag aacacagcac ttcaaagtgc cggccttccc ggaaaaccag
      961 atcgccgcaa tcgcgtttac gtccgggtcg actggaatgc ccgccccgca tcgaaaatca
     1021 tggggtgccc tgacgcgagg ggcaaccgcc gaactggaac ggttcggctt gcatcgcggc
     1081 gagcgcgcgg gactcgtgag taccgtgccg gcgcagcata tgtacgggct ggaatcgacg
     1141 gtattgatgc cgttgcgcgg aggactggtt atgcatgcag gccggccttt ttatccggcc
     1201 gatgtggcgg ctgcactggc acagattccc ggtgatcgcg tgctcgttac gacgcccgtc
     1261 cacttgcgcg cgctcgtgac tgcgggcaca gcactgccga aactcaggct ggtcatttgc
     1321 gccaccgcgc ctctgtcccc tcaattggcc gcgctggtgg aggaccgcta tgaggttccg
     1381 ctgcaagagg tatatggttt taccgaggcc ggcatggtgg cgacccgccg caccgtgcat
     1441 ggtgccatct ggcaatgttt gaaccatgta cggttgcgtc gtgtcggtga tgccgccgcg
     1501 agcgtccggg ggggtcatat cgagactgaa gttgcatgca gcgatttgat tcaaccgctg
     1561 ggcgatcacg actttatcct gcacggacgc aatgccgacc tggtcaatat cgccggcaaa
     1621 cggacctccc tcggtcatct cgaccaccag ctgaacagca tcgaaggcgt ccatgacggc
     1681 gtatttttga tgccagacga gcacggcgaa ggtatcacac gattaacggc atttgtcgtg
     1741 gcgcccgagc ttggcagcga cacagtgctg cgcgccttgc gcgatcgcat agatgaggtc
     1801 tttctgccgc gaccactgta tttcgtcgcc gcgctgccgc gcaatgccac tggcaaactg
     1861 ccacgagaag atctcttgcg gtttgcgaac gattgcgccg cagccgctgc gcgcaaaaaa
     1921 gaagaagtcg ggtgagcact gtacgtttgt tttttgcgat tctggcgatg tgcatcggca
     1981 tgccaggcgt tacagcgccg gctgaccaac ccgcgcgcaa ttggggcgtg gaaatgctga
     2041 tgcagcaact gtcgcaggtc gagcattccc aagcgcgctt tgtcgaacgc aaatatctaa
     2101 aagcactcaa ggcgccgctt gagctgtccg gtacgctctc ctacagccgt cctgatcgcc
     2161 tggaaaaacg cacgctcaaa ccc
//
LOCUS       DATMZQ010000176         9000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_139023, whole genome shotgun sequence.
ACCESSION   DATMZQ010000176 DATMZQ010000000
VERSION     DATMZQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139023"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VN496_05680"
     CDS             <1..317
                     /locus_tag="VN496_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXF16472.1"
                     /translation="FKRLPATLARELVAEPPYSSQRNFGASTLEWCYVAAGRFDVYLH
                     GGQKLWDYAAGSLILEESGGFMCTLQQDDFWSDTLWKRSAIAAREARLFDAWKRWIRA
                     RL"
     gene            413..2137
                     /locus_tag="VN496_05685"
     CDS             413..2137
                     /locus_tag="VN496_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HXF16473.1"
                     /translation="MELTAGVTTELSGAEITLRCLQEEGVEIMFGYPGGAVLPLYDEI
                     FKQEAVKHILVRHEQAAVHAADAYARSTQKVGVVLVTSGPGVTNAVTGIATAYMDSIP
                     VVIITGQVPTHAIGQDAFQEVDAIGVTRPCVKHNFLVKDVKDLAATIKKAFFLAASGR
                     PGPVLVDVPKDVQTAKTNFFYPQSVSMRSYNPVLKGHTGQIRKALNLLLEAKKPMVYT
                     GGGIVLGNASAELTTLVHALGFPCTNTLMGLGGYPATDPQFVGMLGMHGTYEANMAMQ
                     NCDVLLAIGARFDDRVIGNPKHFFQEQRKIIHIDIDPSSISKRVRVDVPIVGDVREVL
                     LEMNRQLAAGKDRADPAALKSWWDQIGVWKSRDCLKYDHLSKIIKPQAVVEKLYELTK
                     DTDMYVTSDVGQHQMWAAQFYKFNKPRRWINSGGLGTMGVGLPYALGVQLAHPDATVA
                     CITGEASIQMCIQELSTAKQYRLPVKIVNLNNRYMGMVRQWQEFFHGNRYAESYMDAL
                     PDFVKLTESYGHVGMRIDKPEDVEPALREAFSRKNDLVFMDFITDQTENVFPMVPGGK
                     GLSEMILV"
     gene            2145..2639
                     /gene="ilvN"
                     /locus_tag="VN496_05690"
     CDS             2145..2639
                     /gene="ilvN"
                     /locus_tag="VN496_05690"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXF16474.1"
                     /translation="MRHIISLLMENEPGALSRVAGLFSARGYNIESLTVAPTEDPSLS
                     RMTVVTTGSDDVIEQITKQLNKLVDVVKVVDLSDGDHIERELMLVKVRASGKDREEMK
                     RMADIFRGRILDVTEKAYTIELTGTGSKLDAFLGALEQGAILETVRTGASGIGRGERI
                     LKVS"
     gene            2726..3739
                     /gene="ilvC"
                     /locus_tag="VN496_05695"
     CDS             2726..3739
                     /gene="ilvC"
                     /locus_tag="VN496_05695"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXF16475.1"
                     /translation="MKVFYDKDADLRLIKSKNVTILGYGSQGHAHSLNLKDSGAKVTV
                     GLRKGGASWDKAKNAGLEVREMADAVKSADFVMILLPDEHIGEVYREIEASLKKGAAL
                     AFAHGFNIHFGQVMPRDDLDVVMVAPKAPGHTVRSTYAQGGGVPMLIAIHQNPSQNAR
                     ELALSYAAAIGGGKAGIIETNFREETETDLFGEQVVLCGGTTALIQAGFETLVEAGYA
                     PEMAYFECLHELKLIVDLIYEGGIANMRYSISNNAEYGDFTRGSRIITEETRKEMRRI
                     LKEIQTGQYAQEFLLENKTGATKLGAMRRIGREHQIEIVGGKLREMMPWIKANRLVDK
                     SKN"
     gene            3822..4487
                     /locus_tag="VN496_05700"
     CDS             3822..4487
                     /locus_tag="VN496_05700"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXF16476.1"
                     /translation="MRAEYPHPIIAREGWPFLATAVGVSLFVSAGWGWLSIPFWLATL
                     FILQFFRDPPRSLPDEPDAVISPADGRVVAVEKSRDPYLDRDALKISVFMNVFNVHSN
                     RSPVDGEVRKGWYFGGKFLNAALDKSSLENERNALWIHTDQGADVTCVQVAGLIARRI
                     LCYVKPNDRLRRGQRFGFIRFGSRVDVYLPSNARPHAAIGDKVFAGQTIVARLSSSTE
                     RST"
     gene            4484..5257
                     /gene="pssA"
                     /locus_tag="VN496_05705"
     CDS             4484..5257
                     /gene="pssA"
                     /locus_tag="VN496_05705"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXF16477.1"
                     /translation="MKPAAPKTRWLSRTRRTGIYWLPNAITTCALFAGFYAIVQAMNL
                     QFELAAKGIFAAMVFDGLDGRVARLTRTQSEFGAEYDSLSDMVSFGAAPALVIYEWSL
                     KGMDKVGWFAAFVYCACAALRLARFNTNIDVLDKRYFQGLPSPAAAGLIAGLVWAMES
                     YDVARADVPLLAWVLTLFAGITMVSSVRFYSGKDINLRKSVPFRVVVMMALVVVALMQ
                     VANNLPEVLFSVFAFYAASGYVMWVWTRIRKKPTPPAVP"
     gene            5461..7173
                     /gene="leuA"
                     /locus_tag="VN496_05710"
     CDS             5461..7173
                     /gene="leuA"
                     /locus_tag="VN496_05710"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXF16478.1"
                     /translation="MIKNPSQKYRPFAPIALADRTWPNNVITQAPVWCSVDLRDGNQA
                     LIEPMDAERKLRLFMLLVKTGFKEIEIGFPAASQTDFDFVRKLIDEDLIPDDVTVQVL
                     TQAREPLIRRTFESLRGVKRAIMHLYNSTSTVQRRVVFGLDKTGIVDIAVQGARAVLD
                     CASEQPQTDWVFQYSPESFTGTELDFAVEICDAVNAVWQPTPKRPVILNLPATVEMAT
                     PNIYADQIEWFGRHIKTRDSIVLSVHPHNDRGCAVAAAELAVMAGADRIEGCLFGNGE
                     RTGNVDLVTLALNLYSQGINPGLDFSDINSVIRTVEHCNQLPVHPRHPYGGELVFTAF
                     SGSHQDAIKKGLSARHPTDLWEVPYLPIDPADLGRAYETVIRVNSQSGKGGIAYLLER
                     DFGLSLPRLLQIEFSQVIQAITDASGKELSAPDIKAAFDREYLKSELPLSYIEHRITH
                     EAGHGEVENVSARLRFNGREITIAGKGNGPVDAFVRALCVGCGIEFHVMNYHEHAVGG
                     GEDATAVAYVQIRIGREQTLYGVGMHGNIVTATLRAVLSAVNRAALRGLITMRDPAQR
                     VAAV"
     gene            7312..8646
                     /locus_tag="VN496_05715"
     CDS             7312..8646
                     /locus_tag="VN496_05715"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXF16479.1"
                     /translation="MASEQKSNRDLHRIVVVGGGAGGLELATQLGRTLGKRKAARITL
                     IDRSRTHLWKPLLHQVAAGSMDLNDHELDYLYQARWHHFQFRLGRMDGLDRANKEVHL
                     APTLDDTGREVLPAHSIAYDTLVIALGSTTNDFGTPGAAVHAISLDNPQQAALFHSRL
                     LNACLRANAQQGPLQAGQLHIAIIGAGATGVELAAELHNTIREFLSFGLDRINPEQDI
                     KITIVEAAPKILPALSERLSAAVVKLLQKLNVEVLTGERVTEVSPRGVKTATGLEIPA
                     ELVVWAAGIKAPDILKDIDGLETNRINQLVVMQTLQTTRDSNIFAFGDCASCPWPGKQ
                     TPVPPRAQSAHQQAAHLSKMLPRHLRGEPLKPFLYRDFGSLISLGKFSTVGSLMGAIT
                     RGSVMIEGLFAKIMYLSLYKMHELALHGFTKVFLDTLARIITRRTEPHIKLH"
     gene            8655..>9000
                     /locus_tag="VN496_05720"
     CDS             8655..>9000
                     /locus_tag="VN496_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16480.1"
                     /translation="MPPGSLKLFPGLAIVAVTACTSIPYSPRPIDADATVAEFLARSP
                     QTDGLRKFAEANGYSQQTWPPQQWDLGELTLLALYFHPDVRIARVRAQVAHAELDSVV
                     QPPPRSARLKPEH"
BASE COUNT         1896 a         2543 c         2658 g         1903 t
ORIGIN      
        1 acttcaaacg ccttcctgca acgcttgcac gcgaactggt agccgagccg ccttattctt
       61 cccagcgcaa ctttggcgcc agcacgctgg aatggtgtta cgtggcggcc ggtcgctttg
      121 acgtgtacct gcatggtggc cagaaacttt gggactacgc ggcaggttcg ctgattctcg
      181 aggagtcggg cggcttcatg tgcacgttgc aacaggacga tttctggtca gacacgcttt
      241 ggaagcgttc ggccattgcc gcacgcgaag cgaggctgtt cgatgcctgg aagcgctgga
      301 tccgggcgcg cctgtgatga ctggggcgtt tccttgaccg atctgggcgt ttgtcggtat
      361 cttgctgggc tttcgcagta atttttccaa cttagcggtt tcaggtgttt ccatggaatt
      421 gactgccggc gttaccaccg agctgtccgg tgccgaaatc acgttgcggt gtcttcagga
      481 agaaggcgtc gagatcatgt tcgggtatcc gggtggcgcg gtcctgcctc tttacgacga
      541 aatattcaag caggaggccg taaaacacat tctcgtgcgg catgagcagg cagcggttca
      601 cgcagcggat gcctatgcgc gttcgacgca aaaggttggc gttgtgctgg tgacgtccgg
      661 tcccggtgta accaacgcag ttaccggcat tgcgactgca tacatggatt ctatccccgt
      721 ggtaatcatc accggacagg tccctacgca tgcgatcgga caggacgcat ttcaggaagt
      781 cgacgctatt ggcgtcacac ggccatgcgt caaacacaat tttctggtca aggatgtcaa
      841 ggaccttgcc gctacgatca agaaggcttt ttttctcgcg gcgagtggac ggccaggccc
      901 cgtgctggtg gatgtgccca aagacgtgca aactgcgaag acgaatttct tctatccgca
      961 gtcggtgtcg atgcgttcct ataatcccgt gctcaaggga cacaccggcc agatcaggaa
     1021 ggcgctgaat ctgctgctgg aagcgaagaa gccgatggtt tataccggtg gcggtatcgt
     1081 gcttggcaat gcgtctgccg agctgaccac cctggtgcat gcactgggct ttccttgcac
     1141 caatacgttg atgggcctgg gcggctatcc tgccactgat ccgcaattcg tcggcatgct
     1201 tggcatgcat ggcacgtacg aagcgaacat ggcgatgcag aactgcgacg tgctgctcgc
     1261 catcggcgcc cgtttcgatg acagagtcat tgggaatcct aagcattttt ttcaggaaca
     1321 acgcaagatc attcacatcg atatcgatcc ttcgtcgata tccaagcgag tgcgtgtgga
     1381 tgttcccatt gtcggcgacg tgcgcgaagt tctgctggaa atgaatcgcc agcttgctgc
     1441 cggcaaagat cgcgcagacc cggcggcact gaagtcatgg tgggaccaga tcggcgtctg
     1501 gaagagccgc gattgcctga agtacgacca cctgtcgaaa attatcaaac cccaggctgt
     1561 ggttgaaaag ctctacgaac tgaccaagga caccgacatg tacgtgacct cggacgtggg
     1621 tcagcaccag atgtgggccg cgcagttcta caaattcaac aagccaaggc gctggatcaa
     1681 ctcgggaggc ctgggcacga tgggcgtggg cctgccttat gcattgggtg tgcaactcgc
     1741 gcatccggac gcgacggtgg cttgcattac tggagaggca agcatccaga tgtgcatcca
     1801 ggagctatcc actgcgaaac agtaccgcct gccggtgaag atcgtgaacc tgaacaatcg
     1861 gtacatgggc atggtgcggc agtggcaaga gttcttccat ggcaaccgct acgccgagtc
     1921 gtacatggat gcattgccgg acttcgtcaa gctcactgaa tcttacggac acgtgggaat
     1981 gcgcatcgac aagccggagg atgtggaacc cgccctgcgc gaggcgttca gtcgcaagaa
     2041 cgatcttgtg ttcatggact tcatcaccga tcagaccgag aacgtatttc cgatggtgcc
     2101 aggcggcaaa ggcctgtcgg aaatgatcct ggtgtaacgg gttcatgcgt cacatcatct
     2161 cccttctcat ggagaacgag ccaggcgcgc tgtcgcgcgt ggccggcctg ttctcggcgc
     2221 gcggctacaa catagagtcg cttaccgttg ctcccacgga agatccttcc ttgtcgcgca
     2281 tgaccgtcgt tacgacaggt tccgatgatg tcattgagca aatcaccaaa cagttgaaca
     2341 agctggtcga tgtggtcaag gtcgtggatc tttccgacgg tgatcacatc gaacgcgaac
     2401 tcatgctcgt caaagtgcgc gcctcgggca aagaccggga ggaaatgaag cgcatggcgg
     2461 acattttccg tggccgtatc ctggatgtta ccgaaaaggc ctacaccatc gagctcaccg
     2521 gcaccgggag caagctcgac gctttcctgg gggcgcttga gcagggcgcc atcctggaaa
     2581 cggtgcgcac gggtgcttcg ggtatcggcc gcggcgaacg catcctgaag gtctcgtgag
     2641 cgaagcaacg cgggactggc gcggccggaa gccaaccagt tcactgcacg accggggact
     2701 taaaatatca atctaagagg tcaatatgaa agttttctat gacaaggacg ccgatctgcg
     2761 tctgatcaaa agcaaaaacg tcacgatcct gggttatggt tcccaagggc acgcccactc
     2821 gctgaacctg aaagattccg gcgccaaagt gacggtgggg ttgcgcaagg gaggggcatc
     2881 ctgggacaag gctaaaaatg ccgggctcga agtcagggaa atggcagacg ccgtcaagag
     2941 cgcggatttc gtcatgattc tgcttccgga cgagcacatc ggtgaggtgt atcgcgaaat
     3001 agaagcgagc ctaaaaaaag gtgcagcgct cgcatttgct cacggcttca atattcattt
     3061 cggtcaagtc atgccgcgag acgatctgga tgtggtcatg gttgcgccca aggcgccggg
     3121 acacacggtg cgttcgacat atgcgcaagg cggcggtgtt ccgatgttga tcgcgatcca
     3181 tcagaatcct tcgcaaaatg cgcgcgaact ggcgctgtcc tatgccgcag cgatcggcgg
     3241 aggcaaggca gggatcatcg aaaccaactt tcgcgaagaa accgaaacgg acctgtttgg
     3301 agaacaagtc gtgttgtgtg gtggaaccac ggcgctgata caagcggggt tcgagacgct
     3361 ggtggaagct ggatatgcgc cggaaatggc gtacttcgag tgcctgcatg agctgaaatt
     3421 gatcgtggac ctgatttacg agggtggcat cgccaacatg cgttactcga tatcgaacaa
     3481 tgcagagtac ggcgatttta cccggggctc gcgcattatc acggaggaaa cgcgcaaaga
     3541 aatgcgcagg atcctcaagg aaatccagac tggacaatac gcacaggaat tccttctgga
     3601 aaacaaaacc ggcgcgacga agctgggtgc catgcggcgc atcggccgcg agcatcagat
     3661 cgagattgtt ggcggcaagc ttcgcgagat gatgccctgg atcaaggcca atcgtctggt
     3721 cgacaagtca aagaactaat gaatgagtga acgggtcaat ggagcgaagg cgagcagttc
     3781 tttgccagtc ctttgctcga tttacgcctt ctacgccagt aatgcgcgct gaatatcccc
     3841 atccgatcat tgccagagag ggctggccct tcctggcgac ggctgtgggt gtttcacttt
     3901 tcgtgagtgc aggctggggc tggttgtcca ttccattctg gctggcgacg cttttcatac
     3961 tgcaattctt ccgcgatccg ccacgctctc tgcctgacga accggatgca gtcatttcgc
     4021 cggccgacgg acgtgttgtc gcagtggaaa aaagccggga tccgtacctg gaccgcgatg
     4081 cgctgaaaat cagcgtcttc atgaatgtgt tcaacgtgca ttcgaaccgc agcccggtgg
     4141 atggcgaagt gaggaagggt tggtattttg gtggcaaatt cctgaacgca gcgctggaca
     4201 agtcttcgct tgaaaacgag cgtaacgcct tgtggatcca taccgatcaa ggcgcggatg
     4261 tcacctgcgt tcaggtggcg ggcctgatcg cacgccggat tctgtgctat gtgaaaccca
     4321 atgaccggct tcgccgcggc cagcgtttcg gcttcattcg cttcggctcc cgtgtagacg
     4381 tctatctccc atccaatgcc agaccgcatg cggcaatagg ggacaaggtg ttcgccgggc
     4441 agaccatcgt ggcccgcttg tcatccagca ctgaacgcag cacttgaagc ctgccgcccc
     4501 aaagacgaga tggcttagcc gaacccgccg taccgggatc tactggctgc ccaatgccat
     4561 tacgacttgc gcgttgttcg ccggcttcta tgcgattgtg caggcgatga atctgcagtt
     4621 cgaactcgcg gccaaaggca tttttgcggc gatggtgttc gacggtctcg acggccgggt
     4681 tgcaaggctc acccgcacgc aaagcgaatt tggtgcggaa tacgacagtc tgtcggacat
     4741 ggtttcgttc ggcgccgccc ctgcactggt catttacgaa tggtccctca agggcatgga
     4801 caaggtcggc tggttcgcgg cgttcgtcta ctgtgcctgt gcagcgctgc gcctggcgcg
     4861 tttcaatacc aatatagatg tgctcgacaa acgctatttt cagggcttgc cgagtcccgc
     4921 cgccgccgga ctcatcgccg ggctggtttg ggcaatggaa tcctacgatg tcgcccgcgc
     4981 tgatgttccg ttgctagcct gggtactgac gctgtttgcg ggaatcacca tggtcagcag
     5041 cgtgcgcttc tacagcggca aggacatcaa tttgcgcaaa agtgtgccat ttcgcgtggt
     5101 cgtcatgatg gcgctagttg tggtggcgct gatgcaggta gccaacaacc tgcctgaggt
     5161 gctgttcagc gtgttcgcct tttacgcggc ttcgggctat gtcatgtggg tttggacgcg
     5221 catacgcaag aaaccgacgc cccccgccgt accgtaaggg cttgcggggc tcaagaatta
     5281 tgcatatagt cgagacatga agtcccaaca gcacggccaa gcccgcattt tcgacgccag
     5341 ccttcggctg gccggcctgc tgctgcgccc cgcgcgcaca taatctaaac aacattcgca
     5401 gtacccaagc ccgccgaaaa attggcggaa cctggcaatc cctttcgtgc gagcgcagcc
     5461 atgatcaaga acccgtcgca aaaataccgt ccctttgccc cgattgcttt ggcagaccgg
     5521 acctggccga acaatgtcat cacgcaggcg ccagtctggt gcagtgttga tttacgcgat
     5581 ggcaaccagg cgctgattga accaatggac gcagagcgca agttgcgcct gttcatgctg
     5641 ctggtaaaaa ccggattcaa ggagatcgag attggtttcc cggccgcatc gcagacggat
     5701 ttcgatttcg tgcgcaagct gatcgatgaa gacctgattc ccgatgatgt cacggttcag
     5761 gtgctgaccc aggcacggga gccgttgatc cgtcgtacgt tcgaatcgct gcggggcgtc
     5821 aagcgggcca tcatgcatct gtacaactcg acttcaacgg ttcagcgccg ggtggtgttc
     5881 ggcctggaca agaccggcat cgtggacatc gcagtgcaag gcgcccgggc cgtactcgac
     5941 tgcgccagtg aacagccgca gaccgactgg gtatttcaat attccccgga gagtttcacg
     6001 ggtacggaac tcgattttgc cgtggaaatc tgtgatgccg taaacgcggt atggcagccg
     6061 acaccgaagc gccccgtcat tctcaatttg ccggcgactg tcgagatggc aacgccaaac
     6121 atctatgccg accagatcga gtggttcggc cgccatatca agacccggga cagcatcgtg
     6181 ttgtcggtgc atccgcacaa cgaccgtggt tgtgccgtgg ctgcggcgga gcttgccgtc
     6241 atggccggtg cagatcgtat cgaaggatgc ctgttcggca acggcgagcg cacgggtaac
     6301 gtcgaccttg tgactttggc actgaatctc tacagccagg gtatcaatcc cggattggat
     6361 ttttcggaca tcaattcggt tatccgcacc gtcgagcact gcaaccagtt gccagtccac
     6421 ccgcgccatc cttacggtgg tgaactcgtg ttcacggctt tttccggttc gcaccaggac
     6481 gctatcaaga aaggcctgtc cgcgcgccat ccgaccgatc tatgggaagt gccctatttg
     6541 cccatcgatc ccgcggattt gggccgcgcg tacgaaaccg ttatccgcgt caacagccaa
     6601 tccggtaaag gcggcattgc ctacctgctc gaacgcgatt tcggcttgtc gctgccgcgc
     6661 ctgttgcaga tcgaattcag ccaggtcatc caggccatta ccgacgccag tggcaaggag
     6721 ctttctgcgc cggacatcaa ggccgcgttc gatcgcgagt acctcaaaag tgaattgccg
     6781 ctctcctaca tcgagcaccg catcacccat gaggctggcc acggtgaagt ggaaaatgta
     6841 tcggcccgcc tgaggttcaa cggccgagaa attaccattg caggcaaagg taatgggcca
     6901 gtggacgcgt ttgttcgtgc gctttgcgtt ggctgcggca ttgagttcca tgtcatgaac
     6961 taccatgagc atgccgtcgg tgggggcgag gacgccaccg cagtggctta tgtgcagatc
     7021 cgcatcggac gcgagcagac tttgtacggt gtcgggatgc atggcaacat cgttacggca
     7081 acgctgcgcg cggtgctttc cgccgtcaac cgcgcggcgc tcagggggtt gatcacgatg
     7141 cgcgatccgg cccagcgcgt cgctgccgtg taacaaagcg caccatcatc aacgagcccg
     7201 ctccctggag cgggcttttc ttcgggtcgg cactatttcc agcaacaagc taaactgcgc
     7261 tggccagcaa aatgctctcc gatcacgggg ccgccttggc cgatccattc aatggcaagc
     7321 gaacaaaaat caaaccgcga cctgcatcga atcgttgtcg tagggggcgg ggccggcggg
     7381 ctcgaattgg ccactcaact gggcaggacg ctggggaagc gcaaggcggc gcgcataacc
     7441 ttgatcgacc gatcgcgcac gcatttgtgg aagccgttgc tgcaccaggt tgccgcaggc
     7501 agcatggatc tgaacgatca cgaactggat tacctgtacc aggcgcgatg gcatcatttt
     7561 cagttccgcc tgggccgcat ggatggcctg gatcgagcca ataaagaggt ccatctcgcc
     7621 cccacgctgg acgacaccgg ccgtgaagtc ctgcccgcgc atagcattgc gtacgatacg
     7681 ctggtgatcg ctctgggcag tactaccaac gatttcggca cgccgggcgc cgccgtccac
     7741 gcgatttcgc tcgacaatcc gcagcaggcg gcgctattcc attcgcgcct gctgaacgcc
     7801 tgtctgcgtg ccaatgcaca acaaggtcca ttgcaggcag gccaattaca tatcgcgatt
     7861 atcggtgccg gtgcgaccgg cgttgaactg gccgcggagc tgcacaacac cattcgcgaa
     7921 ttcctgtctt tcggtcttga ccgaatcaat cccgagcagg acatcaaaat taccatcgtg
     7981 gaagcggctc ctaaaatcct tcctgcgctt agcgagcggc tttctgctgc ggtggtcaag
     8041 ctgctgcaga aattgaacgt cgaagtcctg accggcgagc gcgtcaccga ggtttcgccg
     8101 cgcggtgtga aaaccgccac cgggcttgag ataccggcgg aactggtggt atgggccgcc
     8161 ggcatcaagg cgccggatat cctgaaggac atcgatggtc tggaaacgaa tcgcatcaat
     8221 cagctcgtgg taatgcaaac acttcaaacg acgcgcgact cgaatatttt cgcatttggt
     8281 gattgcgcaa gctgtccctg gccgggaaaa caaaccccgg tgcctccacg agcccagtct
     8341 gcgcaccagc aggcggctca cctcagcaag atgctgccac gccatttgcg tggcgagccg
     8401 ctgaagcctt tcctgtaccg ggattttggc tcgctgattt cgctgggcaa atttagtacc
     8461 gttggcagct tgatgggtgc catcacgcgg ggcagcgtga tgatagaagg cctttttgcc
     8521 aagatcatgt atctgtcgct ctacaagatg catgagcttg cactgcacgg ctttaccaaa
     8581 gtgttcctcg ataccctcgc gcgcatcatc acgcggcgca ccgagccgca tatcaagttg
     8641 cactgattgg tgcgatgccg cctggctccc tgaaactgtt ccccggcctg gccatcgtcg
     8701 cagtcacggc ttgtacttct atcccgtatt cgcccagacc gattgatgcc gacgccaccg
     8761 tcgcggaatt tctagcccgc tcgccgcaga ccgatggtct caggaaattt gccgaggcca
     8821 atggctattc gcaacagacc tggccgccgc agcaatggga tctcggcgaa ttgacattac
     8881 tcgcgctgta tttccatccg gatgtgcgta ttgcacgtgt gcgggcacag gtcgcccacg
     8941 ccgaactgga ctcggtggtg caaccaccgc cgaggtcagc gcggctgaag cccgagcacc
//
LOCUS       DATMZQ010000177         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2142642, whole genome shotgun sequence.
ACCESSION   DATMZQ010000177 DATMZQ010000000
VERSION     DATMZQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142642"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..907
                     /locus_tag="VN496_05725"
     CDS             74..907
                     /locus_tag="VN496_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF16481.1"
                     /translation="MSIFAEPKIDCHVHVIDPANFPYRNDVVYRPAGQEIGTTAQFRH
                     VMDCYNVKHALLVQPNSGYGSDNSCMLDAIEKGEGRFKGIAIIDLDADVATLKQFKAR
                     GIIGAAINPTFHGNDYYRHASVLMKRLADLDMYFNLQVENDQFLMYALWIEEIPVKVL
                     IDHVGRPTPPAGLDQPAFASILRLAETGRVSVKLSGYLKFARSPYPFDDCWPFVRALV
                     DAFTLDHCVWSSDWPYLRAQERQDYGPLVQLVGQLFPNASDRRKLFWDTPNRLFKFAD
                     H"
     gene            936..1514
                     /locus_tag="VN496_05730"
     CDS             936..1514
                     /locus_tag="VN496_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXF16482.1"
                     /translation="MGTQRLEAFTDGVIAIVITIMVLEIKVPHSATPEALLGTLPILL
                     TYVLSFVNVGLFWNNHHHMLHAASRVDGKVLWANLLLLFWLSLVPFVIRWIDESGFQP
                     LPTAAYGAVMAMAAIGYMLLERALINCNGPKSMLAKAVGHDVKSKISLGIYTIAIVLA
                     FYQPWIAIALYIANAAAWFLPDRRIESLKASD"
     gene            1595..>2009
                     /locus_tag="VN496_05735"
     CDS             1595..>2009
                     /locus_tag="VN496_05735"
                     /inference="COORDINATES: protein motif:HMM:NF021230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HXF16483.1"
                     /translation="MAGALFLPAVISNLALAQAPAQTSPEVAINLGGRGWSSPSSNGD
                     ETKRSADEFSFEARGGFATDYMYRGTTLSDHKPAVGAAFEAAFSMFYAGVAVASVKLP
                     TQPSAEISASAGLRKTIADINFDFGATYFLYPGETS"
BASE COUNT          400 a          571 c          573 g          465 t
ORIGIN      
        1 ggcgcgtttc ccactgcggt atggtatgac acgcgcaagc tgattgtgcg tccacgtcat
       61 ccgaccggca gacatgtcca ttttcgccga gccgaagatc gattgccatg tccatgtgat
      121 cgatccggcc aattttccct atcgcaatga cgtcgtctac aggccggccg gccaggaaat
      181 cggcacgacc gcgcaatttc gtcacgtcat ggattgctac aacgtaaagc atgccctgct
      241 ggtgcagccg aattcaggtt acggaagcga caattcctgc atgctcgacg cgatcgagaa
      301 aggcgagggg cggttcaagg gcatcgccat catcgatctc gatgcggatg ttgcgacact
      361 gaagcaattc aaggcgcgcg gtatcatcgg cgccgcgatc aatccgacat tccacggcaa
      421 cgattactac aggcatgcct ccgtcctgat gaaacggctt gccgatctcg acatgtattt
      481 caatcttcag gtcgagaacg accagttcct gatgtacgcg ctgtggatcg aagaaatccc
      541 ggtgaaggtc ctgatcgatc atgtcggacg accaacccct ccggctggtc tcgatcaacc
      601 agcctttgcg tccattctgc gccttgcaga aaccggccgc gtgagcgtca agttgtccgg
      661 gtatcttaaa tttgcacgat caccttatcc gttcgacgat tgctggccct ttgtgcgcgc
      721 gctagtcgat gctttcacgc ttgatcactg cgtatggtct tcggactggc cgtacctgcg
      781 cgcgcaggaa cggcaggatt acggaccgct ggtccagctg gtcggccagc tgtttccaaa
      841 cgcatccgac cggcgcaagc ttttctggga cacgccaaat cgcttgttta agtttgccga
      901 tcactgaagg gagcgcggga atggaaggca aaaacatggg cacgcagcgc cttgaagcgt
      961 ttaccgatgg tgtgattgcc atcgtcataa cgatcatggt cttggaaata aaagtgccgc
     1021 acagcgcgac ccccgaggcg cttctcggca ctcttccgat cctgctcacc tatgtgctga
     1081 gcttcgtcaa cgtgggcctg ttctggaata atcatcacca catgctgcac gccgcctcgc
     1141 gggtcgacgg caaagtcctt tgggcaaact tgttactgct tttttggctt tctctcgtgc
     1201 cgtttgtcat tcgctggatc gatgagtccg gattccagcc gctgccgacc gcggcttatg
     1261 gcgcagttat ggcgatggcg gcgatcggtt acatgttgct ggagcgggcg ctcatcaact
     1321 gcaatgggcc aaagtcgatg ctggcgaaag cagttggcca cgatgtcaaa agcaagattt
     1381 cattgggcat ttacacgatc gccatcgtgc tcgcatttta ccagccgtgg atagccatcg
     1441 cgttgtacat tgccaatgcg gcggcctggt ttctgccgga tcgacgtatc gaatcgttga
     1501 aagcatctga ttaaactcgc gccgcaggct cggatgaggg cgttgttcaa tgtccgttgg
     1561 tgggccaaca aggaaacgtt ctgcggccgt gctcgtggca ggtgcactgt ttttgcccgc
     1621 cgtgatcagc aacctcgcat tggcgcaggc ccccgcgcag acttctcccg aggttgcgat
     1681 caatcttggc ggcagaggct ggtcttcacc gagcagcaat ggcgacgaaa cgaagcgctc
     1741 agccgatgag ttttccttcg aggcgcgcgg cggatttgcg accgactata tgtaccgcgg
     1801 gacaacgttg tcggatcaca agccggctgt tggcgcggcc tttgaagcgg cgttttccat
     1861 gttctacgca ggcgtcgccg tggccagcgt caagctgccg actcagccgt cggctgagat
     1921 ttccgcgagc gccggtttgc gcaagacgat tgcggatatc aatttcgatt tcggggcaac
     1981 ctactttttg tatcccggcg agacatcag
//
LOCUS       DATMZQ010000178         4225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3940661, whole genome shotgun sequence.
ACCESSION   DATMZQ010000178 DATMZQ010000000
VERSION     DATMZQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3940661"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3010)
                     /locus_tag="VN496_05740"
     CDS             complement(<1..3010)
                     /locus_tag="VN496_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF16484.1"
                     /translation="MQTSRKEQPSGALESGPASAGSHLALLDDIAQLLSHAPSEHIAL
                     NTVLQRICTSLGWQYGACWNQDEQSGHVSCSHLWHEPNLAASAFVEISRRTSFEPGAG
                     GLIRTALKTRSPLIVTDIRTLAGLRRASAALAAGLHSAFAFPLLVGGHTLGAMEFFCR
                     DERQRDGALLAAASALSTMVGEFLARCHAESRYRELVELSSDAIIVQYQGTYVFANQA
                     AANVLGADEPSQILGRAAESVVHADCMEKYGAELRQMYENRSGVPMAEVKFVRVDGMP
                     IDVEAASRYIVYAGQAAIQTVFRDITPRKRDEQRIGRLTHLYAALSETSKAITALPSS
                     AQLFREVCRIAVEHGKFALAGILQIETTEKRVGRFVATHGQYQEDLLGIELELDRPGP
                     HLNGPIARAILGDGMHSINNDFLNDHGSRGWRAIAGGANLRSGGAFVLRKGGKVIGAL
                     AVYSPEAGIFDKDLIKLLDEMATTLSFGLEASERDAQRSAAETALLEKERALSTLLGN
                     IPGMAYRCRNDDVWTLEYASEGCIELTGYFPADLIDSRKLPFVHLVYPGDRDRVKNER
                     REKLQRSDHLMIEYQIVCADASVKWVSEKAQPVRDEAGKIIALEGILDDITERKRSEE
                     RLSFLAQYDVLTGLPNRALFYDRLRQAIGRARRENEMIGLMFLDLDRFKQINDTLGHA
                     AGDRVLKVVAERLKRFLREVDTIARLGGDEFTVVIEGVTAPEQLGAIAEKIRSAMAEP
                     VDLDGREMSISSSIGITLYPRDAQDIEQLVKNADIAMYHAKRRGGRQQFQFYEPGMAP
                     PAAEYLELEAKLRRAIEKQEFVLHYQPVVDMASGRVCGMEALVRWQSPQGLISPAKFI
                     AVAEKSGLILDIGTWVLREACTQARKWQREGLPPLRLAVNLSPLQLRQQHLLAAVTEI
                     LRETGLAAQYLELEITENTVMERSPDSMTTLTRLEQLGVRLSVDDFGTGYSSLAYLKQ
                     FPVHVLKIDRSFVRDICTDNDDAAIVRAMIA"
     gene            3121..3759
                     /locus_tag="VN496_05745"
     CDS             3121..3759
                     /locus_tag="VN496_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXF16485.1"
                     /translation="MANNIDKLLASRVIPVLRLDSRKLAESAIDCLVQAGFQTVEITL
                     TTPGAIELIAQLRRNSAEDFLVGAGTVLDLRTAQACLDAGADYLVSPCLVSGMAALAK
                     AAGRIALTGGFTPGEVLSAWRDGAAVVKVFPASTGGASHVAALHAIYPDIPLCPTGGV
                     SSANMLDYFTAGAVIVGIGNSVIHQKALAAGDHAAVIVHARGFLDIAASAPV"
     gene            3762..>4225
                     /locus_tag="VN496_05750"
     CDS             3762..>4225
                     /locus_tag="VN496_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXF16486.1"
                     /translation="MHPIDVVSLGEPLYEFSQIPGSPGRFLQGFGGDTMNCAIAAARQ
                     GARVTYITRLGDDEFGRQFLQLWRDEGLDTSGVAIDKQAHTAVYFITHGPTGHVFNYL
                     RKGSAASRLQARELPFHLLRRARYFHTSGITQAISDSAREAVFAAIEASKGAG"
BASE COUNT          811 a         1286 c         1239 g          889 t
ORIGIN      
        1 tcgcgatcat ggcccttacg atggctgcgt cgtcattatc ggtgcagata tcgcgtacaa
       61 aagagcgatc gatcttgagc acatgcacgg gaaactgttt gaggtaggcc aggctcgagt
      121 aaccggtgcc gaaatcatcg accgacaggc gcacgcccaa ctgctctagc cgggtcagcg
      181 tcgtcatgct gtcgggggat ctttccatga cggtgttttc ggtgatttcc agttccagat
      241 actgggctgc caaaccggtt tcgcgcagga tttcagtcac cgcggcaagc aggtgttgtt
      301 gtcgcagctg cagcggcgag aggttgaccg ccagccgtag cggcggcaac ccttcgcgct
      361 gccatttgcg ggcttgcgta caggcttcac gcagcaccca ggtgccgata tccagaatca
      421 gccccgattt ttccgcgacc gcaatgaact tggcagggga gatcaatcct tgcggacttt
      481 gccagcgcac gagggcttcc atgccgcaga cgcgtccgct cgccatgtcc accaccggct
      541 ggtaatgcag cacgaattcc tgcttctcga tggcccggcg cagcttcgcc tccagttcca
      601 gatactcagc ggcaggcggt gccatcccgg gctcgtagaa ttggaattgc tggcggccgc
      661 cgcgacgttt ggcgtggtac atcgcgatgt cggcgttctt gactagttgt tcgatgtcct
      721 gcgcatcgcg cggatagagc gtgatgccga tgctcgacga aatcgacatt tcgcgtccgt
      781 ccagatccac cggttccgcc atcgcgcttc tgattttttc cgcaatggct cccagctgct
      841 cgggcgcagt gacgccttcg atgacgacag tgaattcatc accgcccagc cgggcaatcg
      901 tatcgacctc gcgcagaaaa cgcttcaggc gctcggcaac gaccttgagc acacggtctc
      961 ccgcggcatg gccaagcgta tcgttgatct gcttgaaccg gtccaggtca aggaacatca
     1021 agccgatcat ttcgttttcg cgccgcgcgc gaccgatggc ttgccgcagc cggtcataga
     1081 acagcgcgcg attcggcaac ccggtcagca cgtcgtactg cgccagaaaa gacaggcgtt
     1141 cctcggaccg cttgcgctct gttatatcgt caagaatgcc ttcgagtgcg atgattttgc
     1201 cggcctcgtc acgcacaggc tgcgcttttt ccgacaccca tttcactgag gcgtccgcgc
     1261 agacgatctg atactcgatc atcagatgat cgctgcgttg aagtttttcc cgccgttcat
     1321 ttttcacgcg gtcccggtca ccgggataca ccaggtgcac gaacggcagc ttgcggctgt
     1381 cgatcagatc cgcaggaaaa tagccggtca gctctatgca tccttcgctg gcatattcca
     1441 gcgtccacac gtcatcattg cgacagcggt aagccatgcc cgggatgttg ccgagcaaag
     1501 ttgatagtgc gcgttctttc tcaagcagcg ccgtttctgc ggcgctgcgc tgtgcatccc
     1561 gttcgctggc ctccagcccg aaagaaaggg tcgtcgccat ctcatcgagc agtttgatca
     1621 ggtccttgtc aaagatgccc gcttccgggg aataaaccgc gagagcgccg atcactttgc
     1681 cgcccttgcg cagtacaaat gcgccgccag agcgcaaatt tgcaccgcct gcaattgcgc
     1741 gccagcctct gctgccatgg tcattcagaa aatcgttgtt gatgctgtgc atgccatcgc
     1801 ccaggattgc gcgtgctatg ggaccgttca gatgtggacc cgggcgatca agctcaagct
     1861 ctatcccgag caagtcctcc tggtattgcc catgcgtggc gacaaacctg ccgacacgtt
     1921 tttcggttgt ctcgatttgc aggatgccgg ccagcgcgaa tttgccgtgc tcgaccgcga
     1981 ttcgacacac ttcgcgaaaa agttgcgcgg aactcggtag tgccgtgatg gccttgctgg
     2041 tttcgctcag tgcggcgtaa aggtgcgtca ggcggccaat gcgttgctcg tcgcgcttcc
     2101 gaggcgttat atcgcgaaat acggtttgaa tagcggcctg accagcgtag acaatgtagc
     2161 gcgaagcggc ttccacatcg attggcatgc cgtcgacacg cacgaatttg acctcagcca
     2221 tcggaacacc ggagcgattt tcgtacatct gccgaagctc ggcgccgtac ttttccatgc
     2281 agtccgcatg caccaccgat tcagccgccc gaccgagaat ttgcgatggt tcgtcagcgc
     2341 ccagtacatt tgcggccgcc tgattggcga acacgtacgt gccctggtat tgcacgatga
     2401 tggcatcgga agacagttca acaagttccc ggtaacggct ttccgcgtgg cagcgcgcaa
     2461 gaaattcgcc caccatggtg ctgagcgcgg aagccgctgc gaggagtgcg ccatcgcgct
     2521 gccgttcgtc ccggcagaaa aattccatgg cgccgagcgt atgtccgccg accagaagcg
     2581 gaaacgcgaa tgccgaatgc agacccgctg caagcgccgc agaagcgcgc cgcaatccgg
     2641 ccagggttcg gatgtccgtg acaatcaagg gggagcgggt cttgagcgcg gtgcgaatca
     2701 aaccacccgc accgggctcg aacgacgttc tgcgcgaaat ctccacgaac gcgctggcgg
     2761 ccaggtttgg ttcgtgccag aggtgcgagc agctaacgtg gccggactgt tcgtcctgat
     2821 tccagcaggc gccgtattgc cagcccaggc tagtgcaaat gcgctgcagg accgtgttca
     2881 gcgctatgtg ttccgacggg gcatgagaca gcaattgcgc gatgtcgtcc agcaacgcca
     2941 gatgagagcc ggcgctcgcc ggtccggatt ccagagcgcc ggagggttgt tccttgcggg
     3001 atgtttgcat gtaaccctga ttatcgccga ttacttcgga cccttcgcta tccagtgggg
     3061 ttatttgctc ctagaatcca gttcccgccg ctacacaacc ctccaacaag cgagctgaaa
     3121 ttggcaaaca atatcgataa gttactggcg tcccgtgtga ttccggtgct ccgattagac
     3181 tcgcgcaagc ttgccgagag cgcaatcgac tgcctggtgc aggccggatt ccagacggta
     3241 gagattacgc tcacgacgcc cggtgcgatc gaattgatcg cgcagttgcg cagaaatagc
     3301 gctgaggatt tcctggtcgg tgcagggaca gtgctggacc ttcgtacggc gcaggcgtgc
     3361 ctcgatgcgg gcgcggatta cctggtatcg ccttgccttg tttccggcat ggcagccctt
     3421 gccaaggcgg cgggccgcat cgcgctaacg ggcggcttta ctccaggaga agtcctgtcc
     3481 gcctggcgcg atggcgcggc cgtcgtaaaa gtttttccgg cctccactgg cggcgcttcc
     3541 cacgtggctg cgctgcacgc catctatccg gacattccac tgtgtccaac gggcggtgta
     3601 tcaagcgcaa atatgctcga ttacttcacg gctggagcgg tcatcgtagg cattggcaac
     3661 agtgtcatcc accagaaagc gctcgcagcg ggcgaccatg cagcagtcat tgtccacgca
     3721 cgcggattcc tcgacatcgc ggcgtctgcg cctgtgtaac gatgcatccg atcgacgtcg
     3781 tatcgctggg cgaaccgctt tacgaattca gccagattcc gggttcgccc ggcagattct
     3841 tgcaaggctt cggaggcgac accatgaatt gcgcaatcgc ggcagcccgg cagggcgcgc
     3901 gtgtcactta tatcacccgg cttggtgatg acgagttcgg ccgccagttt ctgcaactgt
     3961 ggcgcgatga aggtctcgat acttccggtg tggcaatcga taaacaggcg cataccgcgg
     4021 tctatttcat tacgcatggc cccacgggac acgtcttcaa ctacctgcgc aaaggttcgg
     4081 ccgccagccg cttgcaggcc agggagctac cgttccacct gctgcgccgg gcgcgctatt
     4141 ttcatacctc cggcattacc caggcgatca gcgacagcgc gcgcgaggcg gtattcgcgg
     4201 cgatcgaagc ctccaaaggc gcggg
//
LOCUS       DATMZQ010000179         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3526526, whole genome shotgun sequence.
ACCESSION   DATMZQ010000179 DATMZQ010000000
VERSION     DATMZQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3526526"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VN496_05755"
     CDS             complement(<1..191)
                     /locus_tag="VN496_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16487.1"
                     /translation="MMHDSRHDRWRILDYPLSDVDVPISQVSSAPLLWKMRHVTVHPW
                     VLHALGRHGGTLGLIFRNA"
     gene            complement(974..2371)
                     /locus_tag="VN496_05760"
     CDS             complement(974..2371)
                     /locus_tag="VN496_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF16488.1"
                     /translation="MAVDCFRKAIARKPHFAPAHNNLGCALRRLEKVDDAIAAYHQAI
                     ELNPNLADAYNNLGCALRKKDQQDEALRNFRKAIELKPDFADAYNNLAGALIENQPEE
                     AAAAYRRAIEIKPDHVDAHCGLGSVLQASGKFDEASICYCEALRRKPDHAEAHLNNGF
                     LLLTEGKLEEGWREYEWRLKSKICGIQSFKQPLWDGSPLEGRTLLVHAEQGLGDTLHF
                     IRYLPRVFERACGTVRFETLPQLIPLLKQSSYQGLIARGSPLPAFDVHVPLLSLPRIF
                     GTTLTSIPNEVPYLSADSQLVQKWADGLIAYDGLKVGVVWQGNPENKGDRKRSIPLEH
                     FAPLAQVEGVRLFSLQKGPGIEQLKDLSFPVIQFDESIDTQSGPFMDTAAILKNFDLV
                     ISCDTAVAHLAAALGVSTWVALQYLPDWRWMLDREDSPWYPTVRLFRQTESGNWGNVF
                     DRIAHELPNHVGASC"
BASE COUNT          531 a          673 c          739 g          519 t
ORIGIN      
        1 ttggcgttcc gaaagatcaa tccaagagtg ccaccatggc ggccaagtgc atgcaatacc
       61 caaggatgga cagtgacgtg tctcattttc caaaggagcg gcgctgacga gacctggcta
      121 atgggcacgt caacgtcgga cagaggatag tcgagtattc gccacctgtc gtgccggctg
      181 tcgtgcatca tgccaattgc tccaccagaa atcccgaaat gaacgttaat caaaatgagc
      241 cgatggtcgt catgacgctc gtcacgattg gacaataggg gacacccgtc agcgctacgg
      301 aatatggacg ggcctttgaa atgacgagct ctgacaggat ggacaacgct cgacaacgga
      361 tcgccaccgg gtctcgtcaa ccactagtgt ggtagcgact gataggggat agggaagagt
      421 acctggtacg tcgaacttcg aaagttattt cgaatgcagc aagagcagtg cgtttggcaa
      481 cactcgaaat ggacggatgg gggagtgacc tcttgcgata cggtgcgaat tgcatcgcgt
      541 cacggccacg gtttcggtac gtggttcatg catgcagctg aacggaggat ttccgattgc
      601 tcagtaacca gcaacgagga ggggagtggg tcgtcgctag tccgttgatc gcagaaaaat
      661 cttgcagtgc tagaatgaac cggtgccgcg acgctgggct acgcagggca accgcaatgc
      721 ctggttcccg tagcgttcgc aagcaagtag gggagggaag tcttgcccgc gttcaaggcg
      781 gttggagggc gcgtgacatg gattttcttc cagcgggggc ggcccgattg cgcctcagca
      841 ccgccgcgcg ggacacgggc cgcttgcgcg gcacacagat atggcccagt catttaccat
      901 gacgcgcgac ctcaatcatg ctacgacggg cccatggcat aggatctttg ctttgacttg
      961 ttcgggaccg gattcagcag gatgcgccaa cgtggttcgg cagttcgtgg gcgatccgat
     1021 caaagacgtt tccccagttt ccggactcgg tttggcgaaa tagccggacg gtgggatacc
     1081 atggactgtc ctcgcggtcg agcatccaac gccagtcggg aaggtactgc agtgccaccc
     1141 aagtcgatac gcctagcgcc gccgccaagt gcgccaccgc agtgtcgcag ctgatcacca
     1201 gatcgaaatt cttgaggatg gcagccgtat ccataaatgg tcctgattgg gtgtcgatgc
     1261 tttcgtcaaa ctgaatcact gggaacgata agtccttcag ttgttcgatg cccggtcctt
     1321 tctgcaggct gaacaagcgc acgccctcga cttgtgcaag gggtgcaaag tgctcaagcg
     1381 ggatggatcg cttgcggtca cccttgtttt ccggattgcc ctgccagacg acgccgacct
     1441 tcagaccatc gtaggcaatc agtccatcgg cccacttctg gacaagttga gaatccgccg
     1501 acaggtacgg cacttcgttg ggaatggacg ttaacgtcgt tccaaaaatc cgcggcaggc
     1561 tcagcaaagg aacatgcacg tcgaacgccg gcaggggaga acccctggca atcaaaccct
     1621 ggtaacttga ctgcttgagc aggggaatga gctgaggaag ggtttcaaag cggaccgttc
     1681 cgcatgcgcg ctcaaagacc cgcggcagat agcgaatgaa gtgcagcgtg tcgccgagtc
     1741 cctgctcggc atgcaccagg agcgtcctgc cttcaagcgg tgatccgtcc cagagcggct
     1801 gtttgaaact ctgaattccg caaatcttcg acttcaatcg ccattcatac tcgcgccagc
     1861 cttcttccag cttgccctcc gtgagcaata gaaagccgtt gttgaggtgc gcttcggcgt
     1921 ggtcaggctt gcgccgaagc gcttcacagt aacagatgga cgcttcgtca aacttacctg
     1981 acgcctgaag caccgaaccc aagccgcaat gggcgtcgac atggtccggt ttgatctcaa
     2041 tggcccgccg ataggcggcc gccgcttcct ctggctgatt ttcgatcagg gcccctgcca
     2101 ggttgttgta ggcgtccgca aaatcgggct tgagctcgat ggccttgcgg aagttgcgaa
     2161 gtgcctcgtc ctgctggtct tttttgcgca gcgcgcagcc cagattgttg taggcatccg
     2221 ccaggttggg gttcagctcg atcgcctggt ggtaggcggc gatcgcgtca tcgacttttt
     2281 cgagccggcg aagcgcgcag cccaaattat tgtgcgccgg ggcgaagtga ggtttccggg
     2341 caattgcttt gcgaaagcag tcgaccgcca acggcatttg atctcggttc caataggcgc
     2401 aaccaaggtc attgaggata tccgggtcgt ccggcttggc tcgaagcgct ttttgaaaac
     2461 at
//
LOCUS       DATMZQ010000180         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_143967, whole genome shotgun sequence.
ACCESSION   DATMZQ010000180 DATMZQ010000000
VERSION     DATMZQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143967"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..206
                     /locus_tag="VN496_05765"
     CDS             21..206
                     /locus_tag="VN496_05765"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HXF16489.1"
                     /translation="MAHSAATLFAAGDRKRVRKCDNCVLHFHDTSKKGTRRWCSMQLC
                     GNRFKVAAYAARQRENR"
     gene            1015..1212
                     /locus_tag="VN496_05770"
     CDS             1015..1212
                     /locus_tag="VN496_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16490.1"
                     /translation="MDFVRLGNDLFAMPDGSSWFLVDEQKLITLTATMRGVLVDPLKT
                     TIVQNQRCMTTWVITKPQALP"
     gene            complement(1277..2281)
                     /locus_tag="VN496_05775"
     CDS             complement(1277..2281)
                     /locus_tag="VN496_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXF16491.1"
                     /translation="MKRREFVGDFLVGGAMLGARGSGIPKQAGPPPQAAGPATGEGVL
                     VERAASGKPHAGKVLAAIQPHTDDIPIFAGGTVAKLVGEGYTGYLIRTTNDEMAGSGT
                     TGQRVLDNERDTEAVAKALSLQKVFNLEYRNHLMDDISRVEMRARMVHLIRLLKIDTI
                     VCYDPWGIYEENPDHYVTAQVVEASCWMAGMDLDYPEQLAAGLQPHAVQEKYYFARGP
                     QIVNRVVDISAWIDRKVEANLANKTQGPSGENGARLRAKLASQKLRLPILGGDDDSAN
                     RGYIKNIVLDNDSLSQRGVLSDRELGRRYGLEWAEAFHYIGPRESQLERYIADHGVPL
                     "
     gene            complement(2291..>3286)
                     /locus_tag="VN496_05780"
     CDS             complement(2291..>3286)
                     /locus_tag="VN496_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16492.1"
                     /translation="APLNIENVCRVAKSHGVPVFVDAAAETPTFPNHHLARGATMVGY
                     SGGKCMRGPQSAGVLLGQKDLLQAAWLNSAPHHAFGRSLKVGKEEIMGMLAAVEMWAK
                     RDHDAEWKVWESWLSYISDRVKKIPGVTTEVLQPEDLSNHAPRLRINWDGAALNITGT
                     EVVSALMDGSPRITVAGGRGTRPDQMASSVTIMPYMMMPEDYKMAADALHRALSNPPK
                     FSNPVVPAGDPQSVGGVWQVEIDYVRGSAQHRFFVEQTAGTLHGVHEGETLRGDLQGK
                     IQANQIKLSSRHPIQGTVLEYTFTGQVDGDVMHGTVSLGEYGPAEWSAKRHSYSA"
BASE COUNT          688 a          975 c          874 g          749 t
ORIGIN      
        1 ctgcagattt gcttgcgcca ttggcacaca gcgccgcgac attatttgca gccggggatc
       61 gcaaacgcgt ccgcaaatgt gacaattgcg tgcttcactt ccacgacacc agcaagaagg
      121 ggacgcgtcg ctggtgcagc atgcagttat gcgggaatcg gtttaaggtt gcagcgtacg
      181 cagctcgcca gcgcgagaac cgataaaaag gccagctaca tatattcagg gcttagcata
      241 gtaatcgact tattttcttg aattaatacc tacaaacgcc cttgtagtga ccggtctgtc
      301 ctagccttcc gacgcgagat ccgtaagact ctcggtcgtt gacggtggtg atccatcggc
      361 agggtaaaca gttgaaattc tggttgcaca atagttgcac aaatgctccc aaacagggct
      421 ctttgcccaa caatgaggca actcatccga acgtaagcta ttgattccga acaacagcgt
      481 gtggaatcgg gaatttgcta aaccgttgcg cagatgcttt tgaatttgga atcagtagaa
      541 aatcacaaaa gagccgcgtt ccagatacgt tccagttgga cgcaattcct gatgcctgag
      601 cgttttctgc gtgtcgaaaa ttcaacaact tacgtgtttt caatagacct tcatagcccg
      661 attccccccg ccggcaccat gattctaaaa gtacttagct gaactatttt tgctgcgcgt
      721 aactgctacg tagccgaaat cttccttttc tgccgatttt agcgctttta gcgtttctgc
      781 gatcctcaac cagagccaac aatttttgat gtggaaaacg gtcggatgac cgcttctaat
      841 ttggcagact gcgcgagaga tttcggtttt tttctctaag tacttcctgg cgaagcagcg
      901 gatcggtact ccacgctcgc tggaggtgcg cttcgagccg catctggcga tcgcggaccc
      961 acggagcgta gtggtccggg aaatcgccgg aacttggcct ttggatgaat cggtatggac
     1021 tttgtgcgct tgggtaacga tctgttcgcc atgcccgacg gctcgagttg gttcctggtc
     1081 gatgagcaga aactcatcac cctcacggca acgatgaggg gcgtcctggt cgatcccctg
     1141 aaaaccacaa tcgtccaaaa ccagcgttgc atgaccacct gggtcatcac aaagcctcaa
     1201 gctttaccct agcccttgtg ccaccggaag ccagccaatg aacgcgtctg cgagcgccca
     1261 ggcttttggc gcacgattaa agcgggacgc cgtggtcggc gatgtagcgc tcgagttggg
     1321 actcgcgagg gccgatataa tggaaggcct cggcccattc gaggccatag cgacgcccta
     1381 gctcccggtc tgacaagacg ccacgctggc tgagtgagtc gttatccaga acgatattct
     1441 taatgtaccc gcggttggcg ctgtcgtcat ctccgccgag aatcggaagg cgcaatttct
     1501 ggctggcaag tttggcgcgg agccgagcac cgttttcccc gctaggtcct tgcgtcttgt
     1561 tggctaaatt ggcttcgact ttgcgatcga tccaggcgga aatgtccaca acgcgattga
     1621 cgatttgcgg tccgcgcgcg aaatagtatt tttcctgcac cgcgtgcggc tgcaggccgg
     1681 cagcaagttg ctcgggatag tccagatcca ttccggccat ccagcaagac gcttccacaa
     1741 cttgagcggt cacgtaatgg tcaggatttt cctcataaat tccccacgga tcgtagcaaa
     1801 cgatggtgtc aattttgaga agccgaatga gatgaaccat gcgcgcacgc atttcaacgc
     1861 gcgaaatgtc gtccatcaga tgattgcgat attccaggtt gaaaactttt tgcaggctta
     1921 gcgcctttgc gaccgcttcg gtgtcccgct cattgtccaa aacgcgttgg ccagtcgtgc
     1981 cggacccggc catttcatca ttcgtcgtgc ggatcaaata gccagtgtat ccctcaccga
     2041 cgagtttggc gaccgtgcca cccgcaaaga tcgggatatc gtcggtatga ggctggatcg
     2101 cggcaaggac tttaccggca tgaggtttgc cggaagcggc ccgttcgacc aaaacgcctt
     2161 cgccagtggc cggccccgcg gcttgtggcg gaggcccagc ctgtttcggg atgcccgagc
     2221 cgcgcgcgcc gagcatcgcg ccaccaacta gaaaatcacc gacgaactct cgacgtttca
     2281 tcccatggcc tcaggcgcta taggagtgcc gcttggcgct ccattcggca ggcccatact
     2341 cacccagcga aaccgtgccg tgcattacgt cgccatcgac ttgaccggtg aacgtatatt
     2401 ccagcaccgt gccctggatc ggatgccggc tggatagctt gatctgattc gcctgaatct
     2461 tcccctgtaa atctccgcgc agggtctccc cttcgtgaac gccgtgcaac gttcccgcag
     2521 tttgctccac aaagaatcgg tgctgagccg atccccgcac atagtcgatt tcaacctgcc
     2581 agacgccgcc cacgctctgc ggatcgccgg ctggcacgac agggttcgag aacttcggtg
     2641 gattcgaaag cgcacgatgc aaagcgtccg ccgccatttt gtaatcttcg ggcatcatca
     2701 tgtacggcat gatcgtcacc gagctggcca tttggtccgg acgcgtgccc cggccgccgg
     2761 cgaccgtgat ccgcgggctg ccgtccatca gtgcactgac gacctcggtc ccagtgatat
     2821 tcagggccgc gccatcccaa ttgattcgaa gccgcggcgc gtgattcgaa agatcttcgg
     2881 gttgaagcac ctcagtcgtc acaccaggaa ttttcttcac ccgatcgctg atgtagctca
     2941 accacgattc ccagaccttc cactccgcat cgtgatcgcg tttggcccac atttccaccg
     3001 cggcaagcat ccccattatt tcttccttgc cgactttgag cgacctgccg aacgcatggt
     3061 gcggtgcgct gttgagccat gcggcctgca gcaggtcctt ttgccccagc agcacccccg
     3121 cgctttgcgg accacgcatg catttcccgc cgctataccc caccatcgtc gctccgcgtg
     3181 ccaggtgatg gttaggaaat gtcggcgtct ccgccgccgc atcgacgaac acaggcacgc
     3241 cgtggctttt cgcgacccgg cagacattct caatgttcag cggtgc
//
LOCUS       DATMZQ010000181         6205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1455445, whole genome shotgun sequence.
ACCESSION   DATMZQ010000181 DATMZQ010000000
VERSION     DATMZQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455445"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VN496_05785"
     CDS             complement(<1..406)
                     /locus_tag="VN496_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF16493.1"
                     /translation="MSSQRETRKAVIVMVLLVMLWGYSWITSKIGLDYASPMDVVGLR
                     LELGILTLFIGLIWTGRPLKPQHWQALLMIGLIQTGAFLLLNTWALSEGGPGKTSVLV
                     FTMPFWVLVFAWPMLQERIQGLQWVAVALAILG"
     gene            complement(403..1131)
                     /locus_tag="VN496_05790"
     CDS             complement(403..1131)
                     /locus_tag="VN496_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXF16494.1"
                     /translation="MQYVNLAGYKFVSLDALENLKARLKLRSSELGLKGTILLGDEGI
                     NIVIAGSEEGAAAFQVSIHEDSRLSDLRFKRSESAFQPFGRMLVKIKKEIITLRVPGI
                     DPVNRRAPAIAPAELKRWIDEGRDLVLLDTRNAFEVQAGTFEHAMNLGLDSFGQFAEA
                     VDNMDPSLKDKTIVTFCTGGIRCEKAAPVLIKKGFRDVYQLEGGILDYFEKCGGAHFK
                     GRCFVFDQRIALDGNLQAAVGTGE"
     gene            1282..4746
                     /gene="dnaE"
                     /locus_tag="VN496_05795"
     CDS             1282..4746
                     /gene="dnaE"
                     /locus_tag="VN496_05795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXF16495.1"
                     /translation="MRPMQPKFVHLRLHSEYSIVDGIVRLDDAIAAAAADGMPALALT
                     DLSNVFGMVKFYQAARNKGLKPLIGCDVWITNDTDRDKPYRCLLLCTSRAGYLKLCDW
                     LTRAYRHNQYRSRAEIHKQWLGENGTEGLIALSGAHLGDIGHALLADNAAQATKQAQF
                     WSALFPGRFYIELQRPGQPHAEQYVKRAVRLASALKIPVVATHPVQFVKREDFVAHEA
                     RVCIAEGYVLSDQRRPRVFTEEQFLKSQAQMVELFADLPQALDNAVEIARRCSFALEL
                     GKSRLPLFPTPDGRTLEMYLHERALAGLEQRMLALYPDAAAREQQLARYRERLEFEIS
                     TIIQMGFPGYFLIVADFINWAKTNGVPVGPGRGSGAGSLVAYSLGITDLDPLRYGLLF
                     ERFLNPERVSMPDFDVDFCQDGRDRVIDYVRHKYGDDSVSQIVTFGTMAAKAVVRDVG
                     RVMEWNYSRTDELAKLIPFQPGKLITLQMAREMEPRLREREETEEETRELLALAGQLE
                     GLSRNVGMHAGGVLIAPGKLSDFCPLYAASGSENTVSQLDKDDVEAIGLVKFDFLGLT
                     TLTILDWTLRFIKRLDPQANIVLEQLPLDDAASYRIFASANTTAVFQFESRGMRDLLK
                     QARPDRFEDIIALVALYRPGPMELIPDFTQRKQGKQFGYPDPRVESILAETYGIMVYQ
                     EQVMQMAQIIGGYSLGSADLLRRAMGKKKAEEMAQHRSIFREGAGKNGLTPAKADEIF
                     DLMEKFAGYGFNKSHAAAYALVAFQTAYFKAHHTAAFMAANMSAIMDDTDKVQQFHED
                     AIANRLEVLPPDIHASDYRFFPVDNRRIRYGLGAVKGTGQSAIEHIIGARAAGPFKDL
                     FDFCRRVDRRVVNRRVIESLVRAGAFDSINAHRASLLASVGIAMEAAEQASRSASQVS
                     LFGEASTSAEGLKLIEPAAWNESERLQNEKLSLGFYYSGHPFRFYEEEFAAVVRTRLD
                     KLMPPAPEEGARQQNIAGVVESVRMQKTGSGRMMVMMLSDGTARVEVVVYDEILDRHR
                     SLVREDAIVLIEAKVRSFRRGGEDGEENVAMRIAAEKIHDLSGVRARFGRHLKLSMNG
                     AADAKKLKKVLEPYRNGPLRVSIAYTNGNAAAEFDLGDDWKIRPDDNLMLELGKWLEP
                     KNVQIIYQ"
     gene            4707..5234
                     /locus_tag="VN496_05800"
     CDS             4707..5234
                     /locus_tag="VN496_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HXF16496.1"
                     /translation="MAGTKERSDHLSVKQPTLIDSRLIDAVSAQAAASPRRRKNFNFH
                     AQESAPANRLLNAIEPDSYVQPHRHLDPTKDETLVVLRGAVGLVVFDEKGQVLQHSAL
                     RAGTGSIGVDIPHAVWHTFVGLEPGSVIFEAKAGPYLPLARDEKASWAPGEGDPGAVA
                     YLDKLTGLFQTEHSE"
     gene            5257..>6205
                     /locus_tag="VN496_05805"
     CDS             5257..>6205
                     /locus_tag="VN496_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXF16497.1"
                     /translation="MSALDCFHPAVASWFRRTYVAPTEPQVQAWPAISSGRNALIAAP
                     TGSGKTLAAFLATIDSLIRLGVRGELRDEIHVVYVSPLKALSNDIQKNLDEPLAGIRK
                     ELLESGLPDVKIRTVVRTGDTPQSDRDRMRRNPPHILVTTPESLYILLTSESGRKMLS
                     TTRTVIVDEIHAMAPNKRGVHLSLSLERLEHLAGGKLTRVGLSATQKPIEEVARFLVG
                     GGQSNGLFDSNACSIVDTGHVRKRDLALEVPESPLEAVMSAEVWTQVYDRLAELIREH
                     RSTLVFVNTRRLAERAARALSERLGEDQVTSHHGSMAREQ"
BASE COUNT         1331 a         1775 c         1832 g         1267 t
ORIGIN      
        1 atcccagtat ggctaacgcg actgctaccc attgcagccc ctggatgcgc tcttgcagca
       61 tgggccacgc aaataccagc acccagaacg gcatggtaaa caccaatacc gatgtcttgc
      121 cgggtccgcc ttcggaaaga gcccacgtgt tgagcaacag gaaagcgcca gtctgtatta
      181 ggccgatcat cagcagggcc tgccaatgtt gcggcttgag cggccggccg gtccagatca
      241 atccgatgaa caacgtcagg ataccgagtt ccaggcgcag ccccacgaca tccatcgggc
      301 ttgcgtaatc caaaccgatt ttggatgtga tccagctgta tccccatagc atgaccagca
      361 ggaccatgac gatcaccgcc ttgcgcgtct cgcgctgcga actcattcgc cagtgccaac
      421 tgcggcctgc agattaccgt ccagtgcgat gcgttggtcg aaaacgaagc agcgtccctt
      481 gaagtgcgcg ccgccgcatt tctcaaagta gtcgaggatg ccgccttcca gttgataaac
      541 atcccggaaa ccctttttga tcagtaccgg cgccgctttc tcacagcgaa tcccaccggt
      601 gcagaaggtc acgatcgtct tatcttttag cgatgggtcc atgttgtcca ccgcctccgc
      661 aaactgccca aacgaatcca gacccaagtt cattgcatgt tcaaatgttc cggcctgaac
      721 ttcgaaagca ttgcgcgtat ccagcagcac cagatcgcgg ccttcatcga tccagcgctt
      781 gagttctgcg ggcgcaattg caggcgcgcg cctgttaacc gggtcgattc cgggcacgcg
      841 caaagtgatt atttccttct tgatcttgac gagcatgcgg ccgaacggct ggaaggcaga
      901 ttcactgcgc ttgaagcgta ggtcgcttag ccttgaatct tcatggatcg agacctgaaa
      961 tgccgccgcg ccttcttcgc tgccggcgat gacgatattg atgccctcgt cacccagcag
     1021 aatggttccc tttagcccca attcgctgct gcgaagctta agccgggctt tcaggttttc
     1081 gagcgcgtcg agcgaaacga atttatagcc agcaagattg acgtattgca tgaaaaacag
     1141 gccagaacca tcggggccgg tattctacca atcttgcgcg aggcgctgaa cacacaggaa
     1201 atgcagacaa aacaacagtg agccgtaatg cgccggaagc ggtgaacgtt acggcgagcc
     1261 gccaactttt tcgggcgtaa aatgcggccc atgcaaccga agttcgtcca cctgcgtctt
     1321 cacagtgaat actccatcgt tgatggcatt gtccgcctcg acgatgcaat cgctgcggct
     1381 gcagcggacg gtatgccggc actggcgctg accgatttgt cgaatgtatt cggtatggtc
     1441 aagttctatc aggccgcgcg caacaagggg ttgaaaccgc tgattggatg cgacgtttgg
     1501 atcaccaacg acaccgatcg cgacaagcca taccgttgcc tgttgctgtg tacgtcccgt
     1561 gctggttacc tgaagctgtg cgattggctc acgcgagcct atcggcacaa tcagtatcgg
     1621 tcgcgcgctg aaatccacaa gcaatggctg ggggaaaacg gcactgaagg actgatcgca
     1681 ttgtctggcg ctcacctggg tgacatcgga catgcgctgc tggccgacaa cgccgcgcag
     1741 gcaacgaagc aggcgcagtt ctggtcagca ttgtttcccg ggcgctttta tatcgaattg
     1801 caacgcccgg gccagccgca tgcggaacag tacgtcaagc gcgcagtgcg cctggccagt
     1861 gcgctgaaaa tacccgttgt cgccacgcat ccggttcaat tcgtcaagcg cgaagatttc
     1921 gtggcgcacg aggccagggt ttgcatcgcg gaaggctacg tgctttccga tcagcgtcgt
     1981 ccgcgtgtgt ttaccgaaga acaattcctc aagagccagg cgcaaatggt ggagctgttc
     2041 gccgatctgc cgcaagcgct ggacaatgca gtggagatcg cgcgccggtg cagtttcgcg
     2101 ctggagctcg gcaagtcccg tttgcctttg tttcccacgc ccgatggccg cacgctggaa
     2161 atgtacctgc atgaacgcgc gctggcggga cttgaacaaa gaatgctcgc gctctatccg
     2221 gatgcggcgg ctcgggagca acagctcgca cgctatcgcg agcgtctcga attcgagatc
     2281 agcaccatca ttcagatggg gttccccggt tatttcttga tcgtcgccga ctttatcaat
     2341 tgggcaaaga ccaacggcgt accggttggc cccggccggg gttccggcgc aggatcactg
     2401 gtggcgtatt cgcttgggat cacggatctg gacccgctgc gctacggctt gctgttcgag
     2461 cgcttcctga atccggaacg cgtttccatg ccggattttg acgtggattt ttgccaggat
     2521 ggccgcgacc gcgtgatcga ttacgtccgg cacaaatatg gtgatgattc ggtttcgcaa
     2581 atcgtaacct ttggcacgat ggcggccaag gccgtggtgc gcgatgtggg ccgggtcatg
     2641 gaatggaatt acagccgcac tgacgagctg gcaaaactga tcccgtttca gccaggaaaa
     2701 ttaatcacgt tgcaaatggc gcgcgagatg gaaccccgcc tgcgggagcg tgaagaaacg
     2761 gaagaagaga cgcgcgagct gctggcgttg gcaggacagc tggaagggtt aagccgcaat
     2821 gtgggcatgc atgcgggcgg tgtgctaatc gcgcccggca agctgagcga cttttgcccg
     2881 ctttacgccg cgtcagggtc ggagaacacc gtttcacagc tcgacaagga tgatgtcgaa
     2941 gccattggcc tggtgaaatt cgattttctt ggcctgacga cgctgactat cctcgattgg
     3001 acactgcgct tcatcaagcg cctcgatcct caagcaaaca tcgtactgga acagctgccg
     3061 ctggacgatg ccgcgtccta ccgaattttt gccagcgcca ataccactgc cgtgttccag
     3121 tttgaatctc gcggcatgcg cgacttgctc aagcaggcgc ggccggatcg cttcgaagac
     3181 atcattgcgc tcgtcgcgct gtatcggccc gggccaatgg aactgatacc ggatttcaca
     3241 caacgcaaac agggcaagca gtttggttat cccgatccgc gcgtggaatc gattctggct
     3301 gaaacctacg ggatcatggt ttaccaggaa caggtcatgc agatggcgca gatcatcggc
     3361 ggctacagct tgggcagtgc ggatctgctg cgccgggcga tgggcaagaa aaaggccgag
     3421 gaaatggcgc agcatcgcag catatttcga gaaggtgccg gcaagaatgg cttgacgccg
     3481 gccaaggcgg acgaaatctt cgatctgatg gagaagttcg caggctacgg tttcaataaa
     3541 tctcacgccg ctgcgtacgc cctggttgcg ttccagaccg cgtatttcaa ggcgcatcac
     3601 actgcggcat tcatggcggc gaacatgtcg gccatcatgg acgataccga caaggtgcag
     3661 cagttccatg aagatgcgat tgccaaccgg ctcgaggtgc tgccgcccga tatccatgcc
     3721 agcgattacc gtttcttccc ggtagacaac cggcgcatcc gctatggatt gggtgcggtg
     3781 aaaggaacgg gccagtccgc catcgagcac atcatcggag cacgcgccgc cggcccattc
     3841 aaggatttgt tcgatttctg ccgccgcgtc gatcgccgcg tggtgaaccg ccgtgtcatc
     3901 gaatccctgg tgcgcgccgg agcgttcgat tcaatcaacg ctcaccgcgc cagcctgctg
     3961 gcctccgttg gcatcgcgat ggaagcggcc gagcaggcaa gccggtcggc ttcgcaggtg
     4021 agcctgtttg gagaagcttc gacgtcagcg gaagggctca agctcatcga acctgcggcc
     4081 tggaatgagt cggaacgcct gcaaaacgaa aagctctcgc tggggttcta ctacagcggg
     4141 catccgttcc gcttttacga ggaagaattt gccgctgtcg tgcgcacacg cctggacaaa
     4201 ttgatgcccc cggcgcccga agagggtgcc cggcagcaga atattgccgg tgtggtcgaa
     4261 tccgtcagga tgcaaaagac cggcagcgga cgcatgatgg tgatgatgtt gtccgacggg
     4321 acagcgcgcg tggaagtagt ggtctacgat gaaatactgg accggcatcg cagcttggtg
     4381 cgcgaagatg cgatcgtgct gatcgaggcc aaagtacgtt cattccggcg cggcggggaa
     4441 gacggcgaag aaaacgtggc catgcgcatc gctgcggaaa aaatacatga cctatccgga
     4501 gtcagggcgc gcttcggacg tcatttgaaa ttgtcgatga atggcgcggc agatgccaaa
     4561 aagctcaaaa aagtgctgga gccttatcgc aacggaccgc tgcgtgtgtc cattgcttac
     4621 accaacggca atgctgctgc ggaattcgat ctgggcgatg attggaaaat caggcctgac
     4681 gacaatctaa tgctggaact cggaaaatgg ctggaaccaa agaacgttca gatcatctat
     4741 cagtgaagca gccgacgctc atagactcgc ggttgatcga tgccgtttcg gcgcaggctg
     4801 ctgccagtcc gcgacgacgc aagaacttca actttcatgc gcaagaatcc gctcctgcga
     4861 accgcctcct gaatgcgata gaaccggatt cgtatgtgca gccccaccgg cacctcgatc
     4921 cgaccaagga cgagaccttg gtggtactgc gcggcgcggt cggtctggtg gtcttcgatg
     4981 aaaaaggcca ggttctgcag cactccgcct taagggctgg caccgggtcg atcggcgtcg
     5041 acataccgca cgcagtctgg cacaccttcg ttgggctgga accgggatcg gttattttcg
     5101 aggcgaaagc cggtccttat ctgccactgg cccgcgatga gaaagcatcg tgggcaccgg
     5161 gggaaggtga ccctggcgca gttgcgtatt tggacaaatt gaccggtctg tttcaaaccg
     5221 aacattcaga atagtactgg caccaaacgg aaagccgtga gcgcgctcga ttgttttcat
     5281 cccgctgttg cgagctggtt ccgccgcact tatgtggcgc cgactgaacc gcaggtccag
     5341 gcttggccgg cgatcagctc cggtcgcaat gcactgatcg cggccccgac aggttcgggc
     5401 aaaacgctcg ccgcgttcct ggccaccatc gattcgctga tccggctcgg cgtgcgcgga
     5461 gaattgcgtg acgaaatcca tgtcgtttac gtttctccgc tgaaggcctt gtccaatgac
     5521 attcagaaaa acctggatga accgctcgct ggaatccgca aggaactact ggaatcaggc
     5581 ctgcctgacg taaaaatccg aaccgtcgtt cgcaccggtg ataccccgca gtccgatcga
     5641 gatcgcatgc ggcgcaatcc gccgcatatt ctggtgacga cgccggagtc gctgtacatc
     5701 ctgctgacca gtgaatccgg tcgcaagatg ctgtccacaa cccgcactgt gatcgtcgat
     5761 gaaattcatg ccatggcgcc gaacaagcgt ggtgtgcacc tgtcgctttc gctggagcgg
     5821 ctagagcatc tggctggcgg gaagctcacc cgtgtgggac tttctgcgac ccagaaaccc
     5881 atcgaagaag tcgcgcgctt tctggtgggg ggcgggcagt cgaacgggtt gttcgattcc
     5941 aatgcctgca gcatcgtcga tacgggtcat gtgcgcaaac gggatctggc gctggaagtg
     6001 ccggaatccc cactcgaagc cgtcatgtcc gccgaagtct ggacacaagt ctacgaccgg
     6061 ctcgccgagt tgattcgcga gcatcgctcg actttggtgt tcgtcaatac gcgccggctt
     6121 gcggaacgcg ctgcacgcgc gctgtccgag cgtctcggcg aggatcaggt gacttcgcat
     6181 cacggcagca tggcgcgcga gcaac
//
LOCUS       DATMZQ010000182         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4216187, whole genome shotgun sequence.
ACCESSION   DATMZQ010000182 DATMZQ010000000
VERSION     DATMZQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4216187"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VN496_05810"
     CDS             complement(<1..803)
                     /locus_tag="VN496_05810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HXF16498.1"
                     /translation="MWPSLLFLVLLLLPAHALAGAKEKVASLTPAGLVFVVDSAGNEL
                     VAQNADEPFVPASVTKIVTAWLALQVLGGDYRFQTRFYLDKNRALYVRGGGDPFLVSE
                     ELALLAPQLVAAIGKQPINGIVLDASYYPATIRIPGIEDSKESYDALNSALAVNFNTI
                     NAVRNGNSVRSAENQTPITPLAISQFRARGPKGRGRISLSQDPAVSLKYAGELIAAFI
                     GQAGGTVKGPISTGSVPKGLEPIYVHQQSRTLSEILVLLLMASNNYIAN"
     gene            complement(901..>2066)
                     /locus_tag="VN496_05815"
     CDS             complement(901..>2066)
                     /locus_tag="VN496_05815"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXF16499.1"
                     /translation="LVEDINEKPGEGFFPMPLSQDMTTRASSAHAYLTEAVRRRPNLR
                     IMANACVTALQFDGLRAKGASIDHAGKTVEIAAREIILCAGAIHSPAILLRAGIGPAD
                     ELQKIGITPRLDRAGVGRNLQNHPYLHFALTLPRQSRLQNTLRRFAIAGARVSSKLPG
                     APPADLLLFTIGRVSPRSYGPHVAMVGCALYAPCSRGKVSLVSARTDIPPRIEFRLFE
                     DPRDPPRMLVAARFAEDLLCAPGVAATYNDAFLLPPVMSLHQFNRPGVIGNILAVAAK
                     TVLNAPAAVSRHIIGRVIRPGRWFANKHKRHELSDEELLGAAAPMAHPVGTCSIGRAD
                     NPMAVVDPACRVYGICNLRVVDASVMPRVPSANTNLPTIMVAERAADLIRRSK"
BASE COUNT          426 a          598 c          638 g          404 t
ORIGIN      
        1 tggttcgcga tgtagttgtt cgaggccatg agcaaaagga ctagaatctc cgaaagggtg
       61 cgcgactgct ggtgaacgta gatgggctca aggccctttg gcacagatcc ggttgaaatc
      121 gggcccttga cggtgccgcc agcctgcccg atgaacgcgg cgatcagctc gcccgcgtat
      181 tttaggctca ccgcagggtc ctggctcaga ctgatgcgac cgcggccctt tggaccccgc
      241 gcacggaact ggctgatcgc cagcggagtg ataggggttt ggttttcagc ggaacggacg
      301 ctgttcccat tgcgaacggc gttgatcgta ttgaagttca ccgccaaggc tgaattcagg
      361 gcgtcatagg attccttgct gtcctcgatg cccgggatgc ggatggtggc ggggtaatag
      421 ctcgcatcga gcacgatgcc gttgatcggc tgcttgccga tagcggcgac caactggggc
      481 gcgagcagag ccagctcttc agagactagg aaggggtctc caccgccacg cacgtaaagc
      541 gcccggttct tgtccagata aaagcgagtc tggaaacgat agtcaccacc gaggacctgc
      601 agcgccagcc aggcggtcac gatcttggta accgaggccg gaacaaaagg ctcgtcggcg
      661 ttctgggcca ccaactcgtt gccagcggaa tccaccacga agaccagtcc tgccggagtg
      721 agtgacgcca ccttttcctt ggcgccagcc agcgcgtgag cgggcagtag aagaaggaca
      781 aggaagagca ggctaggcca catggcggac cctgttcgtg cgaatgcaaa agaatgcgct
      841 tgccaatgat atgcactcat aacgcctcgc ggacattcag tgtagtcttc catcacggcg
      901 ctatttgctc cgccgaatca aatcggccgc acgctcggca accatgatcg tcggcaaatt
      961 ggtgtttgcg cttggcaccc gtggcatcac agaggcgtcg acgacccgca gattgcatat
     1021 tccataaacc cggcacgccg ggtcgacgac tgccatcgga ttgtcggctc ggccgatcga
     1081 acaagtgccg actggatgcg ccattggcgc ggccgctccg agcagttcct catcgctgag
     1141 ttcatgcctt ttgtgcttgt tggcaaacca tcggcccggc cggatgaccc tgccgatgat
     1201 gtggcggctg acggcggcgg gcgcgtttaa cacagtcttc gcggcgacgg ccaaaatatt
     1261 gccgatgacg ccaggtcggt tgaattggtg cagggacata acaggcggta gcagaaatgc
     1321 gtcattgtaa gtagcggcca cgcctggcgc gcataacagg tcttccgcaa accgcgcggc
     1381 aaccagcatc cgcggtggat cgcgcggatc ttcgaacaat ctgaattcaa tacgcggcgg
     1441 tatgtccgtc cgggcgctca ccagcgaaac cttgccacgc gagcatggtg cgtaaagtgc
     1501 gcacccgacc atcgcaacat gtggaccgta actgcgcggg ctgacacggc cgatcgtgaa
     1561 caacaacaag tctgccggtg gggctccggg cagtttcgag gatacccgtg cgccggcgat
     1621 cgcaaacctt cgcagagtgt tttgcaggcg agattgccga ggcagcgtaa gcgcgaaatg
     1681 cagataaggg tgattttgca aattgcgccc aacgccggcg cgatcgagcc ttggcgttat
     1741 gccgattttc tgcagttcat cggccggccc aattcccgca cgcagcaata tcgccggtga
     1801 gtgaatagcc ccggcgcaca ggatgatctc gcgtgcagct atctccacgg ttttgccggc
     1861 gtgatcgatg cttgcgcctt tcgctctgag gccgtcgaac tgcagcgcgg tcacgcacgc
     1921 attggccata attcgcagat ttgggcgtcg tcgcacggct tcggtcaaat acgcatgagc
     1981 gctcgaggcg cgcgtcgtca tgtcctggct gagcggcatg gggaaaaaac cttcgccagg
     2041 tttctcgttg atatcttcaa caagcg
//
LOCUS       DATMZQ010000183        19848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4216508, whole genome shotgun sequence.
ACCESSION   DATMZQ010000183 DATMZQ010000000
VERSION     DATMZQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19848
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4216508"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /gene="nuoG"
                     /locus_tag="VN496_05820"
     CDS             complement(<1..1906)
                     /gene="nuoG"
                     /locus_tag="VN496_05820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HXF16500.1"
                     /translation="MAKLEIDGRAVEVPDNSTIMDASNKLGIYVPHFCYHRKLSIAAN
                     CRMCLVQVEKAPKPLPACATPVTEGMKVYTRSEAAIKAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASNSRYDEQKRVVSTKELGPLVSAEEMTRCIHCTRCVRFGQEI
                     AGIMELGMAGRGEHAEILPFVARTVDSELSGNMIDVCPVGALTSEPFRYSARTWELAR
                     RKSVAPHDGMGSNLVVQVKGDRVMRVLPLENEDINECWLSDRDRFSYEGLNAGDRLTS
                     PMLKRDGKWEEVDWSTALEFIAGELVRIRRAHGEQAIGALATPQSTLEELYLLGKLAR
                     GCAGGNVDFRLRQADFSADSQLRGTPWLGMKITEIAALDRVLVIGSILRKEQPLLAHR
                     FRQAVKKGAQFNLINASDDDVLMRTAGKAILAPSAWPAMLAQILKAAAGISAVPVPPA
                     VADAVVGDTAHRIAESLLTGLKRAIFLGSLAQHHPRSSELHRMAQELARVIGARMGFF
                     GAGANSVGGYVASATPGGGDVAGMNAGEMLERPRKAYLLLHAEIDFDCGDARAARAAM
                     QSAELVVAMSPFRHGLDYAQVLLPVSPFTETGGSFVNMEGRLQSFEGVVRPLHETRPA
                     WKVLRVLGNLL"
     gene            complement(1916..3265)
                     /gene="nuoF"
                     /locus_tag="VN496_05825"
     CDS             complement(1916..3265)
                     /gene="nuoF"
                     /locus_tag="VN496_05825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXF16501.1"
                     /translation="MNAPLPPFAPDIYGPDAVILKGLTGLNWRLGDYEQRGGYQALRK
                     ILSEKITPEQVVAEMKKSSLRGRGGAGFPTGLKWSFMPRGYAGQKYLLCNSDEGEPGT
                     FKDRDILRYNPHIVIEGMVIAAYAMGITVGYNYIHGEIWEVYERFEQALEEARAAGFL
                     GKNILGSEFSFELHAHHGWGAYFCGEETALLESLEGKKGMPRFKPPFPATYGLYGKPT
                     TINNTETFAAVPWIILNGGEAFLNLGRPNNGGTKVFSVSGHVARPGNYEIKLGTPFAK
                     LLQMAGGMRDGNKIKAVIPGGSSMPVLPGDVMLATDMDYDSIAKAGSMLGSGAVIVMD
                     ETTCMVRALLRLSYFYFEESCGQCTPCREGTGWLYRMVDRIEYGQGRAEDLDKLNSVA
                     DNIAGRTICALGDAAALPVKSFIKHFREEFEYHIEHGKCMVQESNPPRQMNLARAAH"
     gene            complement(3262..3738)
                     /gene="nuoE"
                     /locus_tag="VN496_05830"
     CDS             complement(3262..3738)
                     /gene="nuoE"
                     /locus_tag="VN496_05830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXF16502.1"
                     /translation="MLSPEALALIDKALAKYPADQRQSAVMAALTIAQGEKGWLSPET
                     MDFVAQYLGMPAIAVYEAASFYAMYNLSPVGRNKITLCTNLPCALSGAVVAANQLKQK
                     LGIDFNETTPDGQFTLREGECLGACGDAPVCLHNDKNMLSFMTPDKLDAWIEELRK"
     gene            complement(3750..5003)
                     /locus_tag="VN496_05835"
     CDS             complement(3750..5003)
                     /locus_tag="VN496_05835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXF16503.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAESKTFLQSVPYMDRLDYVSMMCNEHAYVMAIEKLLRIDVPLRAQYIRVMFD
                     EITRILNHLLWLGAHALDIGAMTVFLYCFREREDLMDCYEAVSGARLHAAYYRPGGVY
                     RDLPETMPQYLVSKIHNAKEVEKLNENRRGSLLDFIEDFANRFPRYVDEYETLLTDNR
                     IWKQRTVGIGVVSAERALALGFTGPMLRGSGVEWDLRKKQSYDIYDRVAFDIPVGVNG
                     DCYDRYLVRIEEMRQSNRIVKQCIDWLRKNPGPIIVDDHKVAPPARVDMKTNMEELIH
                     HFKLFTEGMHVPPGEAYAAVEAPKGEFGIYLVSDGANKPYRLKIRAPGFVHLAALDEM
                     VRGHMLADVVAVIGTQDIVFGEVDR"
     gene            complement(5003..5599)
                     /locus_tag="VN496_05840"
     CDS             complement(5003..5599)
                     /locus_tag="VN496_05840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXF16504.1"
                     /translation="MASIAALAERLKDTFGDKVAAMKQECGELTIIVNPADLLYVATE
                     LRDQPQLRFEQLMDLCGVDYLEYAGGQHAGLRYAVVYHLLSVSLNHRLRLKAFAADNK
                     FPVVDSVVGIWPSANWFEREAFDLFGIMFPGHPDLRRILTDYGFIGHPFRKDFPLSGF
                     VEVRYDPEQKRVIYQPVTIEPRETVPRVVREAHYGDVE"
     gene            complement(5609..6085)
                     /locus_tag="VN496_05845"
     CDS             complement(5609..6085)
                     /locus_tag="VN496_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXF16505.1"
                     /translation="MSIEGVTEKGFVTTTIDNVINWTRTGSMWPMTFGLACCAVEMMH
                     AGAARYDLDRFGVVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMSEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGVIQLQNKIRRTNTIAR"
     gene            complement(6192..6560)
                     /locus_tag="VN496_05850"
     CDS             complement(6192..6560)
                     /locus_tag="VN496_05850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXF16506.1"
                     /translation="MLEQYFPILLFIFVGLLVGVALPLAGGTLSRLLGVHRPDPEKLS
                     PYECGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLREIGTFGFLAMLVF
                     LAILVIGFVYEWKKGALEWD"
     gene            complement(6695..6779)
                     /locus_tag="VN496_05855"
     tRNA            complement(6695..6779)
                     /locus_tag="VN496_05855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6743..6745),aa:Leu,seq:gag)
     gene            complement(6853..7242)
                     /gene="secG"
                     /locus_tag="VN496_05860"
     CDS             complement(6853..7242)
                     /gene="secG"
                     /locus_tag="VN496_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXF16507.1"
                     /translation="METAILVFHVLAALCVIGLVLLQHGKGADMGAAFGSGSAGSLFG
                     SSGSANFLSRTTGILAAVFFLTSLGLTYVSSHKGRPEGVMSTHQELPVEPAKSIPAPA
                     QGAPGAPATSTVPAAPDGDSKANEVPK"
     gene            complement(7262..8029)
                     /gene="tpiA"
                     /locus_tag="VN496_05865"
     CDS             complement(7262..8029)
                     /gene="tpiA"
                     /locus_tag="VN496_05865"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXF16508.1"
                     /translation="MVFSVGWRMRQRLAVGNWKMHGSLPENQVLLSKVVAAAGSLGRT
                     LAVVCVPFPYLAQAQSMLAGSKVAWGGQNVSQHPKGAFTGEVSAGMLRELGCRYVIVG
                     HSERRQLFGEDDITVALKASAVLAERMIPVICVGETAAERERGVTEEVVRRQLDVVAD
                     RLGEEGLKASVLAYEPVWAIGTGKTATPQQAQAVHAYLRSLLATSIAAMPILYGGSVK
                     ASNAAELFDMPDVDGGLIGGAALIAEEFIAICRAADG"
     gene            complement(8088..8888)
                     /gene="pstB"
                     /locus_tag="VN496_05870"
     CDS             complement(8088..8888)
                     /gene="pstB"
                     /locus_tag="VN496_05870"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXF16509.1"
                     /translation="MLKNTEGLAAQAALALKAESRNLNFFYGGFQALKGISMPIYDKK
                     VTALIGPSGCGKSTFLRCFNRMHDLYPGNRYEGEIRLHPDNTNLLNPHVDPIEVRMRI
                     SMVFQKPNPFPKSIYENVAYGLRVRGVRGVAAVDEKVEQALRGAALWDEVKDRLNDLA
                     FNLSGGQQQRMCIARALATEPEILLFDEPTSALDPIATASIEELIADLKHKVTILIVT
                     HNMQQAARVSDFTAYMYLGEMIEFDATDTIFIKPKQKATEDYITGRFG"
     gene            complement(8898..9812)
                     /gene="pstA"
                     /locus_tag="VN496_05875"
     CDS             complement(8898..9812)
                     /gene="pstA"
                     /locus_tag="VN496_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXF16510.1"
                     /translation="MTVAEIRSLITRHKRWDLLFATAGVLALMVGVLTFVVLFTGMVV
                     DGAHRLSWDFFATFPSRRAEHAGILSAWVGSTLVMLVTAVTAIPVGVAGAVYLEEYAA
                     KNWMTDVIEINVTNLAGVPSIIYGLLALGLFVYKFGLGQSILSAGLTLALLVLPVVIV
                     ATREAIRSIPAVIREGAYALGATKWQTTAHHILPYSIPGILTGVIIGLARAIGETAPI
                     ITIGALTFIAFLPPAPFSGNPPAGLFDWLFAPFTVMPIQMFNWTSRPESAFHQNAAAA
                     GLVLVVMTLSMNALAIYIRYRLRKKIKW"
     gene            complement(9823..10776)
                     /gene="pstC"
                     /locus_tag="VN496_05880"
     CDS             complement(9823..10776)
                     /gene="pstC"
                     /locus_tag="VN496_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXF16511.1"
                     /translation="MSSVANTRTLNPLADQIVSARLAKKKTRHVSERLVEGLLFLCAF
                     VSVAITVGIVVVLVSESYQFFQQISLVDFLTDNQWTPLFDDTHYGIMVLLSGTLVSSF
                     VALAVAIPLGTMIAIYLSEFAPFSVRELAKPFLELLGGVPTIVYGYFALLYVTPALQF
                     FFPGLPGFNLLSAGLVMGIMIIPYVSSVSEDAMRSVPMAMREGSYAMGATRFQTAIRV
                     VTPAAFSGIAAAYILGISRAVGETMIVAVAAGMQPNLTWNPAEPAATITAYIVQVALG
                     DLPHGSIGYQTIFAAGLSLMLLTLLFNVVGHFMKRRYRQVY"
     gene            complement(10887..11876)
                     /locus_tag="VN496_05885"
     CDS             complement(10887..11876)
                     /locus_tag="VN496_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF16512.1"
                     /translation="MLIFQRTAAAFAVAFCIAVPAHAQLVKIDGSSTVFPITEAIAED
                     FQKSTKGKVKVTVGISGTGGGFKKFCRGETDVSDASRPILKKEMEDCKAAGVQYVELP
                     IAYDALTVVIHPENKWAQTLTVDELKKMWEPAAQGKVKTWKQVNAAWPDQPIKLFGPG
                     ADSGTFDYFTEAVNGKAKSSRGDFTASEDDNVLVQGVSRDVNALGYFGFAYYLENKDK
                     LRAAKIVNKSGQAVEPAFETVINGKYQPLARPIFIYINAKALDRPEVKEFVEFYMKNA
                     EALVREVKYVPLPAKAYAYNVEHTSKKVLGTKFAGENKVGLTIEQLMQLEAKL"
     gene            complement(11987..13351)
                     /gene="glmM"
                     /locus_tag="VN496_05890"
     CDS             complement(11987..13351)
                     /gene="glmM"
                     /locus_tag="VN496_05890"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXF16513.1"
                     /translation="MNKNRKYFGTDGVRGRVGESPITPEFVMRLGYAAGKVLAGEQDL
                     PAGEHASVLIGKDTRISGYMLESALQAGLSAAGVDIYLAGPMPTPAVAYLTRALRLQA
                     GIVISASHNPFEDNGIKFFSADGNKLPDTVEYAIEARLDQPLETMPSALLGKARRLND
                     AAGRYIEFCKSTFPNNLDLRGLKLVVDCAHGATYHVATHVFHELGAEVVAIGAQPDGL
                     NINRDCGATHPQMLARAVKQHHAHLGVAFDGDGDRVTMADQQGKLYDGDQLLFVIAQF
                     RRKLNLLKGGVVGTLMTNLGMEQALAALGIPFARAAVGDRYVLEKLLQRNWQIGGENS
                     GHIICLDKHTTGDGIVSALQVLHALKAEGQGLAQLCAEMRLYPQVLVNVKTMPGFDFE
                     TNDKVTAALKATRAHLGDRGRVLLRASGTEPVIRVMVEGEGQGLIAQLAQSLANTIRQ
                     QTQG"
     gene            complement(13348..14223)
                     /gene="folP"
                     /locus_tag="VN496_05895"
     CDS             complement(13348..14223)
                     /gene="folP"
                     /locus_tag="VN496_05895"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXF16514.1"
                     /translation="MTASSALRLAPLQVLKAGRFSLSLERPLIMGIVNVTPDSFSDGG
                     QFLAPGRALEHATRLVEEGADILDLGGESSRPGAEPIDIQEELRRVLPVLERLVELPV
                     PVSVDTCKPEVMSRAIAAGAAMINDIRALRAAGALETVAGSQVAVCLMHMQGEPATMQ
                     TAPQYDDVVLEVEQFLVERATAALACGIGQERIVLDPGFGFGKTPQHNLELIRALSRL
                     RATGFPVLAGLSRKGLFGKIVGREAAQRVHASAAGALLAAQRGASIVRVHDVAATRDC
                     LLVLRAIEDLAFSLT"
     gene            complement(14357..16249)
                     /gene="ftsH"
                     /locus_tag="VN496_05900"
     CDS             complement(14357..16249)
                     /gene="ftsH"
                     /locus_tag="VN496_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXF16515.1"
                     /translation="MNNLIKNVAIWLVIALVLMTVFNQFSARQQQNTSVEYSQFIEEV
                     KQGNVAKVTIEGRVLKGVKSNGTKFTTYSPSDPWLVSDLLKAGVIIEAKPEEEPSLLM
                     NIFVSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESTNPVTFADVAGCE
                     EAKEEVSELVDFLRDPSKFQKLGGRIPRGVLMVGSPGTGKTLLARAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFETGVGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILM
                     VHMRKVPVAPDVRPDILARGTPGFSGADLANLVNEAALFAARGNKRLVDMDDFERAKD
                     KIMMGAERKSMVMPEHERRNTAYHESGHAVVARLLPRTDPVHKVTIIPRGRALGVTMQ
                     LPAEDRYSMDREQILQNISVLFGGRISEEVFMKQMTTGASNDFERATEMARRMVTQWG
                     MSDHLGPMIYGENEGEVFLGRSITTHKNVSEATMQQVDAEIRRIIDSQYALARKLIED
                     NRDKVEAMAKALMEWETIDADQVGDIMEGRPPRAPKPPTNTQVPPPSQGSAPAPVTAT
                     PAREAK"
     gene            complement(16328..16957)
                     /locus_tag="VN496_05905"
     CDS             complement(16328..16957)
                     /locus_tag="VN496_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HXF16516.1"
                     /translation="MARTRTSKAWMREHVNDVYVQKAKQAGYRSRSAYKLLEIQEKDK
                     LVRPGMTVVDLGAAPGGWSQVLASLVGAPGQVIAVDLLEMEPLRGVELIRGDFLEKAT
                     LELMEQRLGERRIDLVVSDMAPNISGVGLVDQARSIHLAELAFEFARLRLKPGGDLLV
                     KVFQGSGVDAFRKVLSSVFRSVAVRKPKASRDRSSEFYLLARGKTVDMH"
     gene            16988..17359
                     /locus_tag="VN496_05910"
     CDS             16988..17359
                     /locus_tag="VN496_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HXF16517.1"
                     /translation="MPPQLSPAQRRSLKARAHALEPAVLIGRGGMTPAVQAELESSLS
                     VHELIKVRVPGDNRAAREEIMQQICNHLSAAAVQHIGRILVVYRAKPDAAPESNMTKT
                     KTKKTKRKAPRALKRSFQNLA"
     gene            complement(17363..17821)
                     /locus_tag="VN496_05915"
     CDS             complement(17363..17821)
                     /locus_tag="VN496_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HXF16518.1"
                     /translation="MRTITEALQSIAITLWVGGLWVIGLIVAPALFHALSDRSQAGLL
                     AGHFFAVIGYIGLGCGAYLLLFRLARFGGGVFGQLFFWAVLLMAALAAAGQFGLQPMM
                     ASLKQQALPAEVMQSIFRDRFAAWHGVSSIFYLIECFLGLIMVLQHGRGR"
     gene            complement(17906..18382)
                     /gene="greA"
                     /locus_tag="VN496_05920"
     CDS             complement(17906..18382)
                     /gene="greA"
                     /locus_tag="VN496_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXF16519.1"
                     /translation="MTKVPLTVKGAELLRSELHQLKTMERPKVIAAIQEARSHGDLSE
                     NAEYDAAKERQSFIEGRIVEVESKLANAQIIDPAHLDADGRCVFGATVDLEEVDSRET
                     VTYQIVGDDEADIKNNKISISSPIARALIGKYSGDVAEVKAPGGIKEYEILDVKYL"
     gene            complement(18435..>19848)
                     /gene="carB"
                     /locus_tag="VN496_05925"
     CDS             complement(18435..>19848)
                     /gene="carB"
                     /locus_tag="VN496_05925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXF16520.1"
                     /translation="TVSTDYDTSDRLYFEPLTLEDVLEIVAVEKPFGVIVQYGGQTPL
                     KLAQDLDANGVPIIGTNPDMIDCAEDRERFQKMLVELKLKQPPNRTARNPEAALVLAQ
                     EIGYPLVVRPSYVLGGRAMEIVHEQRDLERYMREAVKVSNDSPVLLDHFLNDACEVDV
                     DAICDGEQVLIGGIMEHIEQAGVHSGDSACSLPPFGLSEPVQNELRRQTMLMAKALQV
                     IGLMNVQFAIQNDVVFVLEVNPRASRTVPYVSKATGLALAKIAARCMVGQTLKAQGIG
                     DEVIPPYYCVKEAVFPFIKFPGVDTILGPEMKSTGEVMGVGATFAEAFVKSQLAAGVK
                     LPASGKVFISVRNSDKPKVVEIARTLAKLGFSLLATRGTASALAEVGLQVAPVNKVAE
                     GRPHIVDMIKNGEVAMIVNTVEDRRSAIQDSYSIRRSALQARVTYFTTIAGARAACTG
                     MQHMQELNVYSVQGLHARLH"
BASE COUNT         4330 a         5963 c         5382 g         4173 t
ORIGIN      
        1 gcagcagatt gccgagcacg cgcaatactt tccatgcagg acgggtttcg tgcagcggcc
       61 gcactacacc ctcgaagctt tgcaggcggc cttccatgtt tacgaagctg ccgcccgttt
      121 cggtgaacgg cgataccggc aacaatacct gtgcgtaatc gagcccgtgc cggaacggcg
      181 acatggctac caccagttcc gcgctctgca tggcggcccg ggcggcgcgc gcatcgccac
      241 agtcaaaatc gatttcagcg tgcagcagca aataagcctt gcgcggccgc tccagcatct
      301 ctccagcgtt catgcccgca acatctccgc cgcccggtgt cgcagaagcg acatagccac
      361 cgacgctgtt ggcgccggcg ccgaagaagc ccattcttgc gccaatgaca cgagcgagct
      421 cctgcgccat ccgatgcaat tcgcttgaac gcggatgatg ctgggcgagg ctacccagga
      481 atatggcccg ctttagcccc gtcaacaggc tctccgcgat tcggtgagcc gtgtcgccga
      541 caacggcatc cgcgactgca gggggcaccg gtacagcact gatgccggcc gctgctttca
      601 gaatttgtgc gagcattgcc ggccatgccg agggagctag gatggctttg cctgctgtac
      661 gcatgaggac atcatcgtcc gacgcgttga tcagattgaa ttgcgcgcct ttctttacgg
      721 cctggcggaa acggtgcgca agcagaggct gctctttgcg caagatcgag ccaatgacca
      781 gaacacgatc aagggcggca atttcggtaa tcttcattcc cagccacggt gtcccgcgca
      841 attggctatc tgctgaaaaa tccgcctggc gcagacgaaa atccacattg ccgccagcgc
      901 atccgcgcgc caatttcccc aacaggtaaa gctcttccag tgtcgactgc ggcgttgcca
      961 gcgcgccgat cgcctgttca ccatgcgccc tgcggatacg gacgagttcg ccggcaatga
     1021 actccagggc ggtgctccag tcgacctcct cccatttacc atcgcgcttg agcattggcg
     1081 aagtcaaacg gtcgccggca ttgagtcctt cgtaggaaaa tcgatcgcga tcggacagcc
     1141 agcattcatt gatgtcttcg ttctccagcg ggagaacgcg catgacacga tcgcctttga
     1201 cctgcacgac cagattggaa cccataccat cgtgcggcgc gaccgacttt cgccgcgcca
     1261 attcccaggt acgagcactg taacgaaacg gctcggacgt cagcgcgccg acagggcaaa
     1321 cgtcgatcat gtttccagac agttcggaat ctacggtgcg tgcgacgaac ggcaaaattt
     1381 cggcatgctc gcctcgtccc gccatgccga gctccatgat gccggcaatc tcctggccga
     1441 accgcacaca acgggtacag tgtatgcagc gtgtcatttc ctcggcggac actagcggcc
     1501 ccaattcctt ggtggagacg acgcgtttct gttcgtcata gcgggaattc gatgcgccgt
     1561 agccgaccgc aagatcctgc aactggcatt cgccgccctg gtcgcagatt gggcagtcca
     1621 ggggatgatt gatcagcaga aactccatca cgcccttctg cgccttgatg gcggcctcag
     1681 aacgggtgta gaccttcatg ccctccgtta ccggtgtggc acaagcgggc agtggcttgg
     1741 gtgctttctc gacttgcacg aggcacatgc ggcagttagc cgcaatcgac agcttgcggt
     1801 gatagcagaa atgcggcaca tagataccaa gcttgttgct ggcgtccatg atcgtactgt
     1861 tgtcgggaac ttccaccgcc cgaccatcga tttcaagctt tgccatgtcg taaagtcaat
     1921 gtgccgcgcg cgcgaggttc atttgccggg gaggatttga ttcctggacc atgcatttgc
     1981 cgtgctcgat gtgatattca aattcttcac gaaaatgctt gatgaagctc ttcaccggta
     2041 acgccgcagc atcgccgagc gcgcagatcg tgcgccccgc gatgttatcg gcgaccgagt
     2101 tcagcttatc gaggtcttcc gcacggcctt gtccgtactc gatgcgatcg accatgcgat
     2161 acagccagcc ggtgccttcg cggcacggcg tgcactgtcc acaggactcc tcgaaataga
     2221 aatatgacag gcgcagcaaa gcgcgcacca tgcaagtcgt ttcatccatc acgattaccg
     2281 ctcccgagcc gagcatggaa cccgccttgg caatcgagtc gtagtccatg tccgtagcca
     2341 gcatcacgtc ccccggtaac accggcatgg aggaaccgcc ggggattacc gctttgatct
     2401 tgttaccgtc tcgcatcccg cccgccatct gcaacagttt cgcaaacggc gtaccgagct
     2461 tgatttcgta attgccgggg cgtgcgacat gtcctgagac cgaaaacact ttcgttcccc
     2521 cattgttcgg gcggcccaaa ttcaaaaagg cttcacctcc gttaaggatg atccagggca
     2581 cagcggcaaa tgtttcagtg ttattgattg tcgtgggttt gccgtacaaa ccgtacgtcg
     2641 ccggaaacgg cggcttgaat cgcggcatgc cttttttgcc ttcgagcgac tccagcaaag
     2701 cggtttcctc gccgcagaag taggctcccc aaccatggtg cgcatgcagt tcgaaactga
     2761 actcggagcc caggatattt ttgccgagga aaccggcggc gcgcgcctct tcaagggctt
     2821 gctcgaaccg ctcgtacact tcccagattt cgccatgtat gtagttatag ccaactgtaa
     2881 tacccatggc atacgcggcg atcaccatac cttcgatgac gatgtgaggg ttatagcgca
     2941 ggatgtcgcg atccttgaat gtacccggct caccttcatc cgaattacag agcagatatt
     3001 tctgtccagc atacccacgc ggcatgaagc tccatttcag gccggtggga aaaccggcgc
     3061 cgccacggcc gcgcagcgaa gattttttca tttccgcgac tacttgttcc ggcgttattt
     3121 tctcgctgag aattttgcgc aatgcctggt agccgccgcg ctgttcatag tcgccaagcc
     3181 gccagttcaa tcccgtcagg cccttgagga tgaccgcatc gggaccatag atgtccggcg
     3241 caaacggagg caaaggcgca ttcatttgcg cagttcctcg atccaggcgt ccagcttgtc
     3301 gggcgtcatg aacgacagca tgtttttgtc gttgtgcaga cacacgggtg catcgccgca
     3361 ggcacccaga cattcccctt ccctaagcgt gaattggccg tccggtgtgg tttcgttgaa
     3421 atcaatgcca agcttctgtt taagctgatt cgcagccacc accgcgccag acagcgcgca
     3481 gggcaagttg gtacacagcg tgattttatt gcgcccaacc ggactaaggt tgtacatggc
     3541 atagaaactg gcagcctcgt agaccgcgat cgccggcatg ccgaggtatt gcgcaacaaa
     3601 atccatcgtc tccggagaaa gccagccttt ctcgccttgt gcgatggtca aagccgccat
     3661 gaccgcagat tgacgctggt ccgccggata tttggcgagc gccttgtcaa tgagtgcaag
     3721 cgcctccggt gacagcacca ttggcgcgtt catcggtcta cctctccgaa tactatgtcc
     3781 tgcgtgccga taacggcgac cacgtcggcc agcatgtgcc cccgcaccat ctcgtccagt
     3841 gcagcgagat gcacgaaacc gggcgcgcgg attttgagtc gatacggctt gttcgcgcca
     3901 tccgatacca gataaatgcc gaactcgcct ttgggcgctt ccacggcagc gtaagcctct
     3961 cctggcggga cgtgcatccc ttccgtgaac agcttgaaat ggtgtatcaa ctcttccata
     4021 ttcgtcttca tgtccacgcg agcgggcggc gcgaccttgt ggtcatcgac gatgatggga
     4081 ccaggattct ttcgcagcca gtctatgcat tgcttcacga tgcggttcga ctggcgcatt
     4141 tcttctatgc gaaccagata gcggtcgtaa caatcgccat tcacgccgac cgggatatcg
     4201 aacgccaccc ggtcatagat gtcgtaggac tgctttttgc gaagatccca ttccacgccc
     4261 gacccgcgca acatgggccc ggtgaagccc agtgcgagcg cgcgttcggc agaaacgacg
     4321 ccaataccca ccgtgcgctg cttccagatg cggttgtcgg tcagcagggt ttcatattcg
     4381 tcgacgtagc gcggaaaccg gttcgcaaaa tcctcgatga aatccagcaa tgagccgcgc
     4441 cggttctcgt tcagcttttc aacctctttt gcgttgtgta tctttgagac gaggtactgg
     4501 ggcatggtct cggggagatc gcggtagacg cccccgggac gatagtaggc cgcgtgcagg
     4561 cgcgcccccg ataccgcttc gtagcaatcc atcagatcct cgcgctcgcg gaagcagtac
     4621 aggaataccg tcattgcccc aatatccagg gcatgcgcgc ccagccataa aaggtgattg
     4681 aggatgcgcg ttatttcatc gaacataacg cggatgtatt gcgctcgcag cggcacatcg
     4741 atgcgcagca gcttctcgat ggccatcaca taggcgtgct cattacacat catcgataca
     4801 tagtcgagtc gatccatgta cgggacagac tgcagaaaag tcttggactc cgcgagcttt
     4861 tccgtagccc gatgcagcag gccgatgtgc ggatcggcgc gctggatgac ctcgccatcc
     4921 aattccagaa ccagccggag caccccatgt gccgcagggt gctgcggtcc gaaattcatg
     4981 gtgtagttgc gtatttcagc cactactcga catccccgta atgcgcttcc cgcaccactc
     5041 tcggtacagt ctcacgaggt tcaatggtga ctggctgata gatcacgcgc ttctgttcgg
     5101 ggtcgtagcg gacttcaaca aacccggaga gcggaaaatc cttgcggaaa ggatggccga
     5161 taaagccata gtcggtcagg atgcgtctca aatcgggatg cccggggaac atgattccga
     5221 acaggtcgaa agcctcgcgc tcgaaccagt tcgccgacgg ccagatgcct actacggaat
     5281 ccacaaccgg gaacttgttg tcggcggcga atgccttcag acgcagtcga tgattcaatg
     5341 ataccgacag cagatggtaa accacggcat aacgcaagcc ggcgtgctgt cccccggcat
     5401 actccagata gtccacgccg catagatcca tgagttgctc aaaccgaagt tgcggctgat
     5461 ctcgcaactc ggttgcaacg tataacaggt cggcagggtt gacaatgatc gtcaattcgc
     5521 cgcattcctg cttcatggcg gccactttgt cgccaaacgt gtctttcagt ctttcggcaa
     5581 gcgccgcaat gctggccata ggcaaaattc agcgcgcgat cgtgttggtg cgccggatct
     5641 tgttctgcaa ctggatgaca ccatacagaa gtgcctcggc agtgggcggg catcccggaa
     5701 cataaacatc cacgggcacg atgcggtcgc agccgcgcac cacggaatac gagtagtggt
     5761 agtaaccgcc gccattggcg caggaaccca tggagatcac ccagcgtggc tccgacatct
     5821 ggtcatagac tttgcgcaat gccggcgcca ttttgttgca cagcgtgcca gcaacgatca
     5881 tcacgtcgga ctgacgcggg ctggggcgaa agaccacgcc aaagcggtcc agatcgtagc
     5941 gcgccgcacc tgcatgcatc atctcgactg cgcagcaagc gagtccaaaa gtcatcggcc
     6001 acatcgagcc agtgcgcgtc cagttgataa cattatcaat cgtggtggta acgaatcctt
     6061 tttcggttac gccttcaatg ctcatgaatt gcatctccta cgcagagaga cagccgtgcg
     6121 accacgcagt tcggcaattc gtcgcgcaac aaaaggtgca ttggaagatt cggcgatgaa
     6181 ggatgcttca ctcagtccca ttcaagtgca ccttttttcc attcatacac aaaaccgatt
     6241 acaagaattg ccagaaaaac cagcattgcc aggaatccga atgtgccgat ttcgcgcaac
     6301 acgaccgccc agggaaacag aaatgcaatt tcgagatcaa acaggatgaa caggatggcc
     6361 acgaggtagt agcgcacgtc aaatttcata cgggcgtcct cgaacgcctc aaatccgcac
     6421 tcgtatggtg aaagcttttc cggatcgggc cggtgcacgc caagcagtct gctcagggtg
     6481 ccgcccgcta acggcagggc tactccaacc agtaggccga cgaatataaa gagcaaaatg
     6541 ggaaagtatt gctcaagcat ggtccgcgct cattccatta ccaactgggc gaaacaatcg
     6601 gttccaccca gtgtgcttgg gtaagacatc gattgcattt gccggcgcgc ctcgcgcatt
     6661 tcctcatcca aatgttatct gcggaccatc aacctggtgc cgacggggag actcgaactc
     6721 ccacgggttt ccccaccacc ccctcaagat ggcgtgtcta ccaatttcac cacgtcggcg
     6781 cttctaaatc ctccagccaa tatcaacatc gaggtctttt tcatctttgg cgacgagagc
     6841 acacccattc agctatttcg gtacttcatt ggcctttgaa tcgccatcgg gagcagcggg
     6901 caccgtcgat gtagccggcg cgccgggcgc gccttgtgcc ggtgcaggaa tgctcttggc
     6961 tggttcaacc ggtaattcct gatgcgtgga catgacacct tccggccggc ctttgtggct
     7021 tgaaacgtaa gtcagcccca agctggtcag aaaaaatacc gcggccagga ttccggtagt
     7081 cctgctcaaa aaatttgccg agccgctgga tccgaacagg ctaccagcgg atccactgcc
     7141 aaacgcggcg cccatgtccg cgcctttgcc gtgctggagt agcaccagac caatcacgca
     7201 tagcgcagcc agtacgtgaa aaacaagaat tgcggtttcc atttcatctc atcctgtcgt
     7261 ttcagccatc tgccgctcgg cagatggcga taaattcttc ggctattagc gcagcaccgc
     7321 caatcaatcc accgtcaaca tccggcatgt cgaataactc agcagcattc gatgccttca
     7381 cgctaccccc gtacagtatg ggcatggcag cgatgctggt ggccagcaag ctgcgtaaat
     7441 acgcatgcac tgcctgagcc tgctgtggcg ttgccgtctt gcccgtgccg atcgcccaga
     7501 ccggttcata ggcaagcacc gaagctttca atccttcttc tccaagccgg tcggccacca
     7561 catcgagttg ccgtcttaca acttcttcag tgacgccccg ttctcgctcc gccgccgttt
     7621 cgccaacgca aatgaccgga atcattcttt cagccaagac agcactggcc ttcagcgcaa
     7681 cggtgatgtc gtcttctccg aatagttgtc tgcgctcgga atggccgaca atcacatagc
     7741 gacaacccag ttcacgcagc atccctgcgg aaacctcacc ggtaaaagct cctttgggat
     7801 gttgactgac gttctgaccg ccccaagcca ccttcgaccc tgccaacatt gattgggctt
     7861 gtgctaaata gggaaaaggg acacaaacca cggcgagtgt cctgccaagg ctgcccgccg
     7921 ctgcgacaac cttgctgagg aggacctgat tttccggaag actgccgtgc atcttccagt
     7981 tgcccaccgc cagccgctgc cgcatgcgcc atcccaccga aaaaaccatg gaattttacc
     8041 cgtgagggca aggcagggtc aatgtgggcc ttgatcaggc ggttcaacta gccgaagcgg
     8101 ccggttatat agtcctcggt ggctttctgc ttgggcttga tgaatatggt gtcggtagca
     8161 tcgaattcga tcatctcgcc caggtacata taggcagtga aatcggatac gcgagccgcc
     8221 tgttgcatat tatgcgttac gatcaggatg gtgactttgt gcttcagatc ggcaatcagt
     8281 tcttcgatgc tggcggtggc gatagggtcg agcgcggatg tcggctcgtc gaatagcagg
     8341 atttccggct cagtcgccag agcacgtgca atgcacattc tctgttgctg gccgccggac
     8401 aagttaaagg ccaggtcatt gagcctgtct ttgacctcat cccacaatgc ggcccctcgc
     8461 aatgcctgct cgaccttttc atccaccgca gccacgccgc gtacgccacg tacgcgcagc
     8521 ccgtaggcca cgttctcgta gatggatttt ggaaaagggt tgggcttctg gaacaccatg
     8581 ctaatgcgca ttcgaacctc gatagggtcg acgtgcggat tcagcagatt ggtattatcc
     8641 ggatgcaggc gaatctcgcc ctcgtagcga ttgccgggat acagatcgtg catgcgattg
     8701 aagcagcgca agaatgtcga cttgccgcag cccgaaggcc cgatcaacgc ggtgaccttc
     8761 ttgtcgtaga tcggcatgct gatgcctttc aatgcttgaa agccgccgta gaaaaaattc
     8821 aggttgcggg attctgcctt aagcgcgagc gcggcctgcg cggccaaccc ctcagtgttc
     8881 ttcagcattc cggtctttca ccatttgatt ttttttcgca agcggtagcg gatgtagatg
     8941 gccagcgcat tcatcgacaa tgtcattacg acgagcacca gtccggcggc ggcggcattt
     9001 tgatgaaaag cggattcagg gcgcgatgtc caattgaaca tctggatagg catgaccgta
     9061 aacggcgcga acaaccaatc gaacaatccg gccggcggat tgccactgaa cggtgcggga
     9121 ggaaggaatg caatgaacgt cagtgcgcct atcgtgatga ttggagcagt ctcgccgatc
     9181 gcgcgcgcaa gtccgatgat gacgccggta agaatgccgg gaatggaata cggaagaatg
     9241 tggtgggcag tggtctgcca tttggttgcg ccgagcgcat aagcaccttc acgaatcacg
     9301 gcaggaatcg atcggattgc ctcgcgagtg gccactatga ctaccggcag gaccaataga
     9361 gcgagcgtca gaccggcaga gaggatactt tggccgaggc cgaatttata aacgaacagg
     9421 ccaagcgcca gcaggccata aatgatggac ggcaccccgg caaggttggt cacgttgatc
     9481 tcgatgacat ccgtcatcca gtttttggcc gcatattctt ccaggtaaac cgcgcccgcc
     9541 acgcccaccg ggatggcagt caccgcagtt accagcatga caagcgtact tcccacccag
     9601 gcggacagaa tgccagcgtg ctctgcgcga cgtgaaggaa aggtggcgaa gaaatcccac
     9661 gaaaggcggt gtgccccgtc cactaccatg ccggtaaaga gcacgacgaa tgtcagaacg
     9721 ccaaccatca atgccagtac cccggctgtt gcgaagagca aatcccaacg cttgtgcctg
     9781 gtaataagcg agcggatttc ggcaactgtc atgtcagcca tttcagtaga cctgccggta
     9841 acggcgcttc atgaagtgac ccaccacatt gaacaacaac gtcagcagca tcagacttag
     9901 accggccgcg aaaatcgtct ggtagccgat gctgccgtga ggaagatcgc ccagcgcgac
     9961 ctgcacgata taggcggtaa tggtggcagc cggttccgcc gggttccagg tcaagttcgg
    10021 ttgcatgccg gcagcgacgg ctacgatcat ggtctcaccg actgcgcgcg aaatgccgag
    10081 aatgtatgct gccgcaatcc cggaaaacgc agcaggcgtc acgacccgaa ttgcagtctg
    10141 aaaacgtgtt gcgcccattg cgtaggagcc ttcgcgcatc gccatgggta ccgaacgcat
    10201 tgcatcctcc gagacagagc ttacataagg gatgatcatg atccccatga ccaatccggc
    10261 ggatagaaga ttgaacccgg gaagacctgg gaaaaaaaac tgcagggccg gggtcacata
    10321 aagcaacgca aaatagccat agacaatggt cggcacgcct cccagcaatt caagaaacgg
    10381 cttggcaagc tcgcgcaccg agaagggcgc aaattctgac aaatagattg caatcatggt
    10441 gcctagcggt attgcgacgg cgagcgccac aaaagagctc accagcgtcc cggagagcaa
    10501 taccatgatg ccgtaatgcg tatcatcgaa tagcggtgtc cattgattgt cggtaaggaa
    10561 atcaaccaac gaaatctgct ggaaaaactg ataagactcg ctcaccagga ctacgacaat
    10621 gcccacggtg atcgcgactg aaacgaaggc acaaaggaac agcaagcctt caaccaagcg
    10681 ctcgctgaca tgccgggtct tctttttcgc gagccgcgcg ctgacgatct ggtccgccaa
    10741 gggattcagt gttcgggtat ttgctacaga gctcatcggt tcaggaccgg ggaataatat
    10801 tacagcggac gcccaacaac cgcagccggg gaaaaaacag gaggggcatg cccctcctgt
    10861 tccgttccgg ccagctcacc cacagttcaa agcttggctt cgagctgcat caactgttcg
    10921 atcgtaaggc cgaccttgtt ttcgccagcg aacttggtgc cgagcacttt cttgctggtg
    10981 tgctccacat tgtaggcgta agccttcgcc ggcagaggta cgtactttac ctcgcgcacc
    11041 agcgcttcgg catttttcat gtaaaactcg acaaactcct tcacttccgg ccggtccagc
    11101 gcctttgcat tgatatagat aaatattgga cgcgccagcg gctgatattt gccgttaatg
    11161 accgtttcga aggcgggttc cactgcttgt ccggacttgt ttacgatctt ggccgcccgc
    11221 aatttgtcct tgttttcgag gtagtaggca aacccaaaat agccaagcgc gttgacatca
    11281 cgcgatacgc cctgcaccag cacgttatcg tcttctgagg cggtgaagtc gccgcggctc
    11341 gactttgcct tgccatttac ggcttcggtg aaataatcaa aagtaccgga atctgcccca
    11401 gggccgaaca gtttgatcgg ctgatccggc catgcagcat tgacttgttt ccaggttttc
    11461 actttgcctt gtgcggctgg ctcccacatt ttcttgagct catcgacggt aagggtctgc
    11521 gcccacttgt tttcgggatg gataacgacg gtcagcgcgt cataggcaat tggcagttcg
    11581 acgtattgca cccctgcagc cttgcaatcc tccatttcct ttttcaggat gggacgcgag
    11641 gcatcagaga catcggtctc cccgcggcaa aatttcttga atccgcctcc cgttccagaa
    11701 atgccgacag tgactttgac ttttcctttt gtcgatttct ggaaatcctc cgcgatcgct
    11761 tcggtaatcg gaaacaccgt gctggaacca tcgatcttca ccaattgagc atgcgctggc
    11821 acagcgatgc aaaaggcgac cgcaaaggca gcggccgtgc gttggaatat gagcatcagg
    11881 gcgatctcct tacgatatgg ttttcgggtc gaccgctatt ctatggaggc gatgtgacac
    11941 tttcattgca cgcccgggag gcggtctgcc acgcagccgc tgctgttcag ccttgagttt
    12001 gctgccgaat ggtgttcgcc aacgactgcg ccagttgcgc gataagcccc tgtccttcac
    12061 cctcgaccat gacccgaatg accggttcgg tgcccgatgc ccgcagcagc acccgaccgc
    12121 gatcaccgag atgtgctcgg gtcgccttga gcgcggcagt gactttgtca ttggtttcga
    12181 aatcgaagcc aggcatcgtt ttgacattca ccagaacttg cggatacagc ctcatctcgg
    12241 cacacaactg cgcgagcccc tgaccttccg ccttcagcgc atgcaacact tgcaatgcgg
    12301 acacgatacc gtcaccggtc gtatgcttgt ccaggcagat aatgtgtccc gaattttctc
    12361 ctccgatctg ccaattccgt tggagtagtt tctccagcac atagcggtcg ccaaccgcgg
    12421 cacgtgcgaa cgggataccg agcgcggcga gcgcctgctc catccccaga ttggtcatta
    12481 atgtgccgac cactccgccc ttcagcaagt tgagcttcct gcgaaattga gcaataacaa
    12541 acaacagctg gtcgccgtca tacagtttgc cctgctgatc cgccatcgtg acgcgatcac
    12601 catctccatc gaatgccacg cctagatgcg catgatgctg tttcactgcc cgtgcgagca
    12661 tctgcggatg cgtagcgccg caatcgcggt ttatgttgag gccatctggt tgcgcaccta
    12721 tggcgaccac ttcggcgccc agttcgtgga acacatgcgt tgcgacgtgg taggtcgcgc
    12781 cgtgggcgca atccaccacc agctttagcc cgcgcaagtc gagattgttc ggaaaagtac
    12841 ttttgcaaaa ctcgatgtaa cgtcccgccg catcgttgag gcggcgcgcc ttgccgagca
    12901 gcgcggaggg catggtctcc aggggctggt ccaggcgggc ctcgatcgcg tactcgaccg
    12961 tgtccggcaa cttgttgccg tccgcagaga agaacttgat gccattgtct tcgaagggat
    13021 tgtgcgaagc cgatatgaca atgccggcct gcaggcggag cgcccgggtc aggtacgcga
    13081 ccgcgggagt aggcattggg ccggcaagat aaatatcgac gccggcggcc gacagaccag
    13141 cctgcaaagc tgattcgagc atgtaaccgg atatgcgagt gtctttgccg attagcaccg
    13201 aagcatgttc gcccgccggc aaatcctgct ccccggcaag cactttgccg gcggcgtagc
    13261 caaggcgcat gacgaactcg ggggtaatcg gcgattcacc gactcggcca cgcacgccat
    13321 ccgtaccgaa atattttctg tttttgttca tgtgagtgaa aacgccaagt cctctatcgc
    13381 gcgcagtacc agcaggcagt cgcgggtcgc cgcgacgtcg tgcacacgga caatcgaagc
    13441 gcctcgctgg gctgccaaca gcgctcctgc cgcgctcgca tgcacgcgct gcgcggcttc
    13501 acgcccgaca attttaccga atagaccctt gcgcgacagg cctgccaata ccggaaatcc
    13561 ggttgcgcga agccgggaca atgccctgat caattcgaga ttgtgctgcg gcgtcttgcc
    13621 gaaaccgaat ccagggtcca gaacgatgcg ctcctgtcca atgccgcaag ccaaagccgc
    13681 agtagcgcgt tcaaccagaa actgctccac ttcgagtacc acatcatcgt attgcggcgc
    13741 cgtctgcatg gtcgccggtt cgccctgcat gtgcatcagg cacaccgcga cttgggagcc
    13801 cgctaccgtt tcaagcgccc ctgccgcacg caacgcgcga atgtcgttga tcatcgccgc
    13861 tccagctgca atggcgcggc tcatgacttc aggtttgcag gtgtccaccg aaaccggcac
    13921 tggtaattct acgaggcgtt ccaatactgg cagcacacgc cgcagctctt cctggatgtc
    13981 gatgggctcg gcaccgggcc gcgacgattc accgcccaga tccagaatat ctgcaccttc
    14041 ttcaaccaat cttgttgcgt gttccagagc gcggccagga gcgaggaatt ggccgccatc
    14101 cgaaaaagaa tcaggcgtta cattaacgat gcccatgatg agagggcgtt ccagcgatag
    14161 cgaaaagcgg ccggccttaa gaacctgcag cggcgcaagc cgcaaggcgg aagaggcagt
    14221 catttgcatt gcgactgatg ctgtagaaac gcaaagggtg aggcgtcggc ctcaccctat
    14281 cattgttcta atccgcatga ttgcggtttc ggaaatcgct tcgcttatgc ctgctgatcg
    14341 gtgcccaatg tctatttcat ttcgcttccc gcgctggcgt cgcagtgacg ggcgccggtg
    14401 cgctgccctg ggacggaggc ggcacttgcg tgttagtggg tggcttgggc gcacgcggcg
    14461 gacggccttc catgatatcg cccacttgat cagcgtcgat tgtctcccac tccatcagcg
    14521 ctttcgccat ggcttcgacc ttgtcgcggt tgtcttcaat cagcttgcgg gccagggcat
    14581 attgactgtc gatgatgcgc cgaatctccg catcgacctg ttgcatggtc gcctcggaaa
    14641 cattcttgtg cgtggtgatg gatcggccca aaaagacttc tccttcattt tctccgtaga
    14701 tcatgggtcc caggtggtcg ctcatacccc attgggtaac catgcgccgc gccatttccg
    14761 tggcgcgttc aaaatcgttc gaagcacccg tggtcatttg tttcatgaac acttcttcag
    14821 aaatacgtcc gccgaacaac acggaaatat tctgcagaat ctgctcacga tccatgctgt
    14881 agcgatcctc cgccggcagc tgcatggtca cgccgagtgc tctgccacgc ggaataatgg
    14941 tgactttgtg caccgggtcc gtccgcggca gcagccttgc aacgaccgca tggccggatt
    15001 catgataagc agtattacgg cgctcatgct ccggcatgac catggactta cgctcggcgc
    15061 ccatcatgat tttgtctttg gcgcgttcga aatcatccat gtccaccagg cgcttgttgc
    15121 cgcgtgccgc gaatagcgca gcctcgttga cgagattggc aaggtccgcg ccggaaaagc
    15181 ccggcgtgcc gcgcgcgaga atatccgggc gaacgtcggg cgcaaccggc accttgcgca
    15241 tgtgaaccat caagatctgc tcgcgccccc ggatatctgg caacggaacc actacctgac
    15301 gatcgaaacg tcccggtcgc agcaaagctg gatccagcac atcggggcgg ttggttgcag
    15361 cgatcacgat aacgcccacg ccagtttcga agccatccat ttcgaccagc agttggttca
    15421 gcgtctgttc gcgttcgtca ttgcccccac ccaggccggc gccgcgttga cggcccacgg
    15481 cgtcgatttc atcaataaac acgatgcaag gcgcgtgctt tttggcttgc tcgaacatgt
    15541 ctcgtacgcg tgccgcaccg acgccaacga acatttcgac gaaatccgaa ccggaaatcg
    15601 agaagaaggg cacttttgcc tcgccggcga tcgcccgtgc cagcagtgtc ttgccggtac
    15661 ccggggagcc gaccatcagc acgccgcgtg gaatgcgccc gccgagtttc tggaatttgc
    15721 tggggtcgcg caggaaatcg accagctcgg agacttcttc cttggcttcc tcgcagccgg
    15781 cgacatcggc aaaggtcacc ggattagtgg attcgtccag catgcgggcg cggctttttc
    15841 caaacgagaa tgcgccgccg cggccacccc cttgcatctg gcgcataaag aaaatccata
    15901 cgccgatcag caacagcatc gggaaccagg atacgaaaat attcatcagc agggacggct
    15961 cttcctccgg cttggcttcg atgatgacgc ctgctttcag aagatcggac accagccatg
    16021 gatcagaggg cgagtaagtc gtgaacttcg tcccgttgct cttcaccccc ttcagcacgc
    16081 gtccctcaat cgtgactttg gccacatttc cctgcttcac ctcctcgatg aattgggaat
    16141 actccacgga agtgttctgt tgctggcggg cgctgaactg gttgaagacc gtcatcagta
    16201 ccagggcgat gacaagccag atcgctacgt tcttgataag gttattcaag gtttctcctt
    16261 gcagcggcga ccctcgagcc gccaatcaat gaagtcattc taaacccatt cctgcgcccg
    16321 agccctgtca atgcatatca accgtcttgc cacgagcgag aaggtaaaac tcgctggacc
    16381 ggtctcgcga ggctttaggc ttgcgcaccg cgaccgagcg gaaaacactg gaaagcacct
    16441 tgcggaatgc gtccacgccg gagccctgaa acactttgac tagcaggtca ccccccggtt
    16501 tcagccgcaa gcgcgcaaat tcgaacgcca gctctgccag atgaatggaa cgggcttggt
    16561 ccacaaggcc taccccgctg atattgggcg ccatgtcgga aaccacaagg tctattcgac
    16621 gctcgcccag ccgctgttcc attagctcca gcgtggcctt ttccagaaaa tcgccgcgaa
    16681 tcagctcaac gccgcgcagt ggttccattt cgagcaagtc caccgcgatc acttggcctg
    16741 gcgctccaac cagcgatgca agcacctgag accagccgcc gggcgcagcg ccaagatcga
    16801 ccaccgtcat gccaggccgt accaacttat ccttttcctg tatttcgagc agcttgtatg
    16861 ccgaacggga acgataaccc gcctgcttcg ccttttgcac gtacacatcg ttcacatgct
    16921 cgcgcatcca cgccttgctg gtcctggtac gagccattgc ctaaaatgcc tttttgagga
    16981 acgaaccatg ccgccacaat tgtcgcccgc acaacgtaga agcttgaagg ctcgcgcgca
    17041 tgcgctggaa ccggccgtgc tgatagggcg aggcggcatg acgccagccg tgcaggccga
    17101 attggaaagc agcctcagcg tccacgaact gatcaaagta cgcgtgccgg gcgacaatcg
    17161 cgcagcgcgc gaggagatca tgcaacaaat ctgcaaccat cttagcgccg cagctgttca
    17221 gcatatcggc agaatccttg ttgtttacag ggcgaagccg gacgcggcgc cggaatcaaa
    17281 tatgacaaaa acaaaaacga aaaaaacaaa aagaaaagca ccgcgagcac tcaagcgcag
    17341 tttccagaat cttgcctagc tgctagcgtc cgcgcccatg ctgcaacacc atgatcaggc
    17401 ccaagaaaca ctcaatgagg tagaagatgc ttgagactcc gtgccaggcc gcaaaacggt
    17461 cgcggaatat gctttgcatg acctcggcgg gcagcgcctg ctgtttcaag ctagccatca
    17521 tgggctggag gccaaactga cccgctgcag ccaaagccgc catcagcaat accgcccaaa
    17581 agaacaattg accgaaaacc ccgcctccaa agcgtgccag cctgaacaag agcaaatagg
    17641 cgccacaacc gagcccgatg tagccaatga ctgcaaagaa gtgaccggca agcaggccgg
    17701 cctgggaacg gtcgctcagt gcgtgaaata acgcgggcgc aacgattagc ccgatgaccc
    17761 agagtccacc tacccaaagg gtaatggcaa tcgactgcag cgcttcggta atggttctca
    17821 cgggtacgga cgatttcagt gatgcgcgag caattaagca aaaacctgca agtacaagcc
    17881 tggctacgtc aaaaggcgct aaaggctaca aatatttgac gtccaggatt tcgtactcct
    17941 tgattccacc cggtgctttg acttccgcga catcgccgga atatttgccg atcagcgcgc
    18001 gtgcgattgg cgagctaatg gagatcttgt tgtttttgat atctgcctcg tcgtctccga
    18061 caatctggta ggtcacagtt tcgcggctgt ctacctcttc cagatccaca gtcgcgccga
    18121 acacgcagcg tccatcagca tccaaatggg ccggatcgat gatttgcgca ttggccagtt
    18181 tgctttccac ttcaacaatg cgcccctcga taaagctttg cctttccttt gcggcgtcgt
    18241 attcggcgtt ctcggataaa tcgccgtgtg aacgtgcctc ctggatggcc gcaatcacct
    18301 tgggccgttc catggtcttc agctgatgca attcgctgcg cagcaattca gcgcctttca
    18361 cagtcaatgg aactttagtc atcattcatg cccttcagag ttgatggatc gcctgctccc
    18421 cgcaggagcg acgttcagtg cagccgggcg tgcaacccct gcaccgaata aacattgagt
    18481 tcctgcatgt gttgcatgcc ggtgcaggcg gcgcgagccc cggcgatggt tgtgaaatac
    18541 gttacgcgcg cctgcagggc ggagcgcctg atcgaatagg agtcctgaat ggcgctgcga
    18601 cgatcctcca ccgtgttcac gatcatcgcg acttcgccat tcttgatcat atcgacgatg
    18661 tgcgggcggc cttccgccac tttgttcacc ggcgccacct gcaatccgac ttccgccagc
    18721 gcgctagctg ttccgcgcgt cgcgagcaaa ctgaagccca acttcgccag cgtgcgcgcg
    18781 atttcgacta ccttcggttt gtccgaattg cgcacgctga taaatacctt accgcttgcc
    18841 ggcaacttga cccccgcggc aagctgagat ttgacgaacg cttctgcaaa tgtggcacct
    18901 acgcccatga cttcgccagt tgatttcatc tccggaccca ggatggtgtc cacacccggg
    18961 aatttgatga agggaaatac cgcctccttt acacagtaat acggcggaat cacttcatca
    19021 ccaatgccct gggctttcaa cgtctggcca accatgcagc gggcggcgat tttggctagt
    19081 gccagcccgg tagctttgga cacgtaaggt accgtacgtg acgcgcgcgg attgacttcc
    19141 agaacgaaca ccacatcgtt ctgaatggcg aactgcacgt tcatcaggcc tatgacctgc
    19201 agcgccttgg ccatcaacat ggtctgccgg cgcagctcgt tctggacagg ctccgacaaa
    19261 ccgaaaggcg gaagcgagca tgccgaatcg ccggaatgca cgcctgcctg ttcgatatgc
    19321 tccatgatgc cgccgatcag cacctgctcg ccatcgcaaa tggcatctac gtccacttca
    19381 caggcatcat tgagaaaatg gtccaacaac acgggagaat cgttggagac tttcaccgct
    19441 tcgcgcatgt agcgttcgag gtcgcgctgt tcgtgcacga tttccattgc ccgcccgccg
    19501 agcacgtaag acggccgcac caccagcgga tagccgattt cctgggccag caccagtgcc
    19561 gcttcgggat tgcgcgcggt gcggttcggc ggctgcttga gcttcaattc caccagcatc
    19621 ttctggaaac gctcgcggtc ctccgcgcaa tcgatcatgt ccggattggt gccgatgatc
    19681 ggtacgccgt tggcgtccag gtcctgcgcc agcttgagcg gcgtctgtcc gccgtactgg
    19741 acgatgacgc cgaagggctt ttccaccgcg acgatttcga ggacgtcttc cagggtcagc
    19801 ggttcgaaat acaaccggtc cgacgtgtcg tagtcggttg acaccgtc
//
LOCUS       DATMZQ010000184         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_144930, whole genome shotgun sequence.
ACCESSION   DATMZQ010000184 DATMZQ010000000
VERSION     DATMZQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144930"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="arsS"
                     /locus_tag="VN496_05930"
     CDS             complement(<1..705)
                     /gene="arsS"
                     /locus_tag="VN496_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HXF16521.1"
                     /translation="MHATLPLLEVTGFPALHRHQLTTLQVNLGYRCNQSCLHCHVNAG
                     PTRTEMMDAQTLDLIPEVLKARSITTLDVTGGAPELHEGFRDLVRTARALGVEVIDRC
                     NLTVLFEPGQETLAEFLAAQRVQVVASLPCYSLENVDNQRGKGVFDKSIAALQKLNAL
                     GYGRPDSDLTLNLVYNPQGPSLPPEQGKLQAAYKKELFEHFGIVFDELFTLTNMPIQR
                     FGSMLISKGQFNQYMHL"
     gene            complement(758..1894)
                     /locus_tag="VN496_05935"
     CDS             complement(758..1894)
                     /locus_tag="VN496_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF16522.1"
                     /translation="MKRLLLVGGGHAHLSVLDTLAREQPGGVEAVLVTPSRFQIYSGM
                     LPGWMAGHYVQRECEIDLLPLAQLARVRVVKDRVAGLDADRHRIHLEDGEPIEYDLLS
                     LDVGSETDVAELQRAGDKLLPVKPLDAFFSAWPEIVSAARHERGYRLVIVGGGAAGVE
                     LALAAHYAFGRSGIDARIDLVVSDAGLLAGHARGVQRRIARVLDSSGIVVHPLQAHGT
                     EAGVILSDGTRLPADRIIAATGARAPGWLKGSGLRLDRNAYVAVDGTYRSVSHPNVFA
                     AGDVSARNDAVVGRSGVHAVHAGPVLGQNLLAVLRGGRMQTYQPRRHSLYLLACGRRY
                     AVASWGPWSAEGAWVWYWKDWIDRHFVQHFETASARATSGRRVS"
     gene            2184..>3290
                     /locus_tag="VN496_05940"
     CDS             2184..>3290
                     /locus_tag="VN496_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF16523.1"
                     /translation="MDFEHSQKVKDLQKRLNAFMDEHIYPNEARFFREAEEVGPLKVA
                     PVIEEVKKKGKAAGLWNLFLPHSEHGAGLTNLEYAPLCEIMGRSLLAPEAFNCSAPDT
                     GNMEVLARYGTDEQKKKWLTPLLAGEIRSCFAMTEPGVASSDATNIESSIVRDGNDYV
                     INGRKWYATGSIDPRCKIFIFMGKTDANNPDRHKQQSMILVPRDTPGVDVQRALPVLG
                     FYGMPDRTGEVVFQNVRVPAANILLGEGRGFEIAQGRLGPGRIHHCMRLIGLAERTLE
                     AMCKRTLNRVAFGKPVAEQTVTLERIAESRIGIAQSRLLTLHAAHMMDTVGNKGAKAE
                     IAMIKVAVPNMACKVVDWAIQAFGAAGLSNDHGIG"
BASE COUNT          705 a          974 c          981 g          630 t
ORIGIN      
        1 aaggtgcatg tattgattga actgcccctt cgaaatcagc atcgaaccga agcgctggat
       61 cggcatattc gtcagggtga acaattcgtc gaacacgatg ccgaaatgtt cgaataattc
      121 tttcttgtat gcggcctgca gcttgccctg ctcgggcggc aggcttggcc cctgggggtt
      181 gtaaaccaga ttaagcgtaa ggtcggaatc cggacgaccg tagccgagtg cattcagctt
      241 ttgcaacgct gcaatgctct tgtcgaagac gcccttgcca cgctggttgt ccacgttttc
      301 cagtgaatag cagggcaatg acgccactac ctgaacgcgt tgggccgcca gaaattccgc
      361 cagcgtctcc tgacccggct cgaacaggac ggtgagatta cagcggtcga tgacttcaac
      421 cccgagtgct cttgctgtgc gcaccagatc gcgaaatccc tcgtgcagct caggcgcacc
      481 gccagtcaca tccagcgtgg ttatgctgcg cgccttcaac acttcgggaa tcaggtcgag
      541 cgtttgcgca tccatcattt cagtgcgcgt ggggccggcg ttcacatggc aatgcaggca
      601 gctctggttg cagcggtagc ccaaattgac ttgcagggtg gtgagttgat ggcgatgcag
      661 tgccggaaaa ccggttacct cgagcaacgg tagtgtggcg tgcattacga tcctttgaat
      721 accaaacgcc ccgcggggtc cgggcggtat cttacaccta gctgacgcgc cggccgctgg
      781 ttgcgcgtgc cgacgcagtt tcgaagtgtt gcacaaagtg ccggtcgatc cagtctttcc
      841 agtaccacac ccacgctcct tcggcgctcc agggtcccca cgatgccacg gcatagcgtc
      901 ggccgcatgc cagcaagtac aaggaatggc ggcgtggctg gtaggtctgc atgcggccgc
      961 ctcttagtac agccagcagg ttttggccga gcactggacc ggcgtgaact gcatgcacgc
     1021 ccgagcgtcc cacgacagcg tcgttacgtg cgctgacatc gcctgctgcg aacacattcg
     1081 gatgcgagac actgcgatag gttccgtcaa cggcgacgta agcattccgg tccagccgca
     1141 atccggagcc tttgagccat cccggcgctc gtgcaccggt cgcggcaatg atccggtcgg
     1201 ctggaagccg cgtaccgtcc gacaatataa cgcccgcttc agtcccgtga gcctgcaggg
     1261 ggtgaacgac tatgcctgag gaatcgagaa cgcgtgcaat ccggcgttgg acgccgcgcg
     1321 catgccccgc cagcaatcct gcatcagaaa ccactagatc gatacgagca tctatgcccg
     1381 agcgtccgaa tgcataatgc gcggcgagag cgagttccac gccggccgcg ccgccgccca
     1441 cgatgaccag gcgatagcca cgctcgtgac gcgcggccga cacaatctcc ggccacgcac
     1501 tgaaaaacgc atccaggggc ttgaccggca aaagtttatc gcccgcacgc tgcagctccg
     1561 caacatcagt ctcgctgccg acatcgagcg acaacaaatc gtattcgatc ggctccccat
     1621 cctccaagtg aatgcgatgc cgatcggcat ccagccccgc gacccgatcc ttgactacac
     1681 gtactcgagc taattgcgcc agcggcagca ggtcgatctc gcattctcgc tggacgtagt
     1741 gccccgccat ccagccgggt aacatgcccg agtagatttg aaagcgcgaa ggcgtaacca
     1801 ggacggcttc gactccgcct ggctgttcgc gggccagcgt gtcgagcacg gaaagatgtg
     1861 catgccctcc gcccaccaga agcaggcgct tcatggtgca atcaggccgc gcacggcctt
     1921 acatcaactc cacgaccatg ttcagcggat ccttgagtca tcaacgttgc gactgctttc
     1981 ctgcactggg aaagtgataa atgccggcag aataccgggt ggaccagtgg ggacatagta
     2041 cgtcaggatg ggtcgaaccg gaaaatagcg gcgtgtgtaa cagtcgatag tagaatgcgc
     2101 ggtttggttt cgaggtggcg tttcgcagcc gatgccgcgt ggcattggaa atgccaatcg
     2161 cgattacgaa tcagagggaa ggcatggatt ttgagcattc gcagaaagtg aaggatctcc
     2221 agaagcggct gaacgcgttc atggacgagc acatctatcc gaacgaagcg cgtttttttc
     2281 gggaagccga agaggtcggg ccgctcaaag tggccccggt catcgaggag gtgaagaaga
     2341 aaggcaaagc agcgggactg tggaatctgt ttctgccgca ctccgagcat ggcgccggct
     2401 tgaccaacct ggagtacgcg cctctgtgcg agatcatggg gcgctcgttg ctggcaccgg
     2461 aagccttcaa ttgctctgca ccggataccg gaaacatgga agtgctggcc cgttacggga
     2521 ccgacgagca aaagaaaaaa tggctgacac ctttgctggc cggtgagatt cgttcgtgtt
     2581 ttgcgatgac ggaacctggc gtagcgtcga gtgacgcgac gaacatcgaa tcatccattg
     2641 tccgcgacgg caacgactat gtaatcaacg ggcgcaagtg gtatgcgact ggctccatcg
     2701 atccgcgctg caagattttc atttttatgg gcaagaccga tgcgaacaat cccgatcgac
     2761 acaagcagca atcgatgatt ctggttccgc gcgatacgcc cggggtcgac gtgcagcgcg
     2821 cgctgcccgt gcttggcttc tacggcatgc ccgaccggac cggcgaagtt gttttccaaa
     2881 acgttcgcgt gccggccgca aacatcctgc taggcgaagg acgtggtttc gaaattgcgc
     2941 agggacggct gggcccgggc cgcattcacc attgcatgcg gttgatcgga ctcgccgagc
     3001 gcacgctcga agcgatgtgc aagcgcacct tgaaccgggt cgcattcggc aagccggtcg
     3061 ccgaacaaac cgtcacgctg gaacgcatcg ccgagtcgcg catcggcatc gcccagagcc
     3121 gcttgctgac attgcatgcc gcccacatga tggacaccgt cggcaacaag ggcgccaagg
     3181 ccgaaattgc catgatcaaa gtggcggttc cgaacatggc gtgcaaagtc gtcgattggg
     3241 caatccaggc gttcggtgct gccgggctca gcaacgatca cggcatcggt
//
LOCUS       DATMZQ010000185        11515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2907862, whole genome shotgun sequence.
ACCESSION   DATMZQ010000185 DATMZQ010000000
VERSION     DATMZQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2907862"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VN496_05945"
     CDS             complement(<1..855)
                     /locus_tag="VN496_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXF16524.1"
                     /translation="MNRGAIRIPYTFAKAQGVVVTAMDGDIAQVAVRTDAHAGALAEL
                     RRALGVPLNARRIAPEQFDELIAATYNGADAGAVALAGDLAQDFDLSRLLQEIPRIED
                     LLESQDDAPVIRLINALFTQALRDGASDIHVEPFETRSVVRLRIDGTLRDLIEPARAL
                     HGAIVSRVKIMAQLDIAEKRLPQDGRITLRIAGKPVDVRVSTIPTGHGERVVLRLLDK
                     QAGRLDLGRLGMDDATLAHMDRLIQEPHGIVLVTGPTGSGKTTTLYAALSRLDAATLN
                     IMTVEDPIE"
     gene            complement(852..3101)
                     /gene="gspD"
                     /locus_tag="VN496_05950"
     CDS             complement(852..3101)
                     /gene="gspD"
                     /locus_tag="VN496_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HXF16525.1"
                     /translation="MLLLLVASLAICVVPLAHSQERPKDAAKVPARPADSKPAARTAT
                     PAVKGMPAEAPPPSPAAPSRPPREAPDVVTLNFVNADIEGVVKVVSEITGKNFVIDPR
                     VKGTVNIVSAKPMPRSRVYDVFLAALRLQGFAAVEDRGLVKVVPEADAKLHSSPTQAQ
                     GETGGISGDRIETRVFTLKYESAVQALPILRPLIAPSNTITAYQNNNTLVITDYANNL
                     ERIADIIDAIDQPNANDPVVIPLKYASAVDVAATVNRLFAEAAQAQGAAEPTQRFTVV
                     ADARSNSLLARSGDTSRLARLRKFIAILDSPTNAGGNIHVVYLKNAEAVKLAEILRAI
                     YQGEGGTTAAAPSSTPTAQRTSLGANAPMGTPQSQSQLGGQGTSGVGAASSFSINQAQ
                     TTPTAPGIIQADASTNSIIITAPDAIYNNLRAALDKLDVRRAQVYVEALIAEVGANKA
                     AEFGIQWQDLNGYNKNSAQPFGGTNFGGAGQNIIGIAQNPGTAGQGLNVGIIKGQVNV
                     PGVGQILNLGFLVRALQTDSSSNILATPTLLTLDNEEAVILIGQNVPFVTGQYALSGG
                     ATTPTPFQTVERHDVGVTLRVKPQISEGGTVRLQIYQEVSSIEDATNPAGVITNKRAV
                     ESTILVDDGRIVVIGGLIQDTVLDGVQKVPVLGDIPILGALFRYKTRSHVKTNLMVFL
                     RPTLVRSSQRADEYTGERYDYILGEQQKAKPAHDVVLPDVQSPLLPPREQTPALQIEV
                     KPYNNEGRK"
     gene            complement(3182..3685)
                     /locus_tag="VN496_05955"
     CDS             complement(3182..3685)
                     /locus_tag="VN496_05955"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HXF16526.1"
                     /translation="MNRFHRGMLAMQLPFDRSNLAQRALVAVVTIAALTAFSLVAAYW
                     TWTWFAPRAEWRAQAGAGNSAGAAVGTLFGAAAKERNAAAPTGIAIRLLGIVAATAGR
                     QGYAVMEVEPRQILAVHEGDDVAPGIRLAEVEADHVTLERNGRQETLTWPEKSKPAEP
                     APVRTNR"
     gene            3870..4301
                     /gene="gspG"
                     /locus_tag="VN496_05960"
     CDS             3870..4301
                     /gene="gspG"
                     /locus_tag="VN496_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXF16527.1"
                     /translation="MRCLKGQRGFTLLEVMVVVVILGILAALVVPKIISRPDEARIIA
                     AKQDIASLLQALKLYRLDNHRYPSSEQGLQALVVQPTGTPVPPNWKAGGYIERLPKDP
                     WGNAYQYLNPGVHGEIDVFSLGADGAPGGEGNDADIGSWSL"
     gene            4396..4908
                     /locus_tag="VN496_05965"
     CDS             4396..4908
                     /locus_tag="VN496_05965"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXF16528.1"
                     /translation="MPVSLRQSGFTLIEMLVVLLIMGIFVGLVSTITQPDDHAMLKLE
                     AQRLARLLDLAATKSRLTGRPTAWTADAHSYRFWQFSEDAFWTEILDDDTLRARVLPQ
                     DMAILRLEVENRKSTDQMRLEFTPYGQPVSFSLQMGLGDARETVVGSPIGEVSVLSRQ
                     EATNEPTVHR"
     gene            4886..5281
                     /gene="gspI"
                     /locus_tag="VN496_05970"
     CDS             4886..5281
                     /gene="gspI"
                     /locus_tag="VN496_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HXF16529.1"
                     /translation="MSPRCTARRTGFTLVEVLVALAIVSIALLAALRVAGGGTNSVGD
                     LRARLLAEWVAQNVLAEQRARAEWPSLGILRGTQLQGGVQFAWREEVIATPNVMFRRV
                     DIQVFATAEDAHSLAHLTGFVVNAPVPNR"
     gene            5278..5880
                     /locus_tag="VN496_05975"
     CDS             5278..5880
                     /locus_tag="VN496_05975"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HXF16530.1"
                     /translation="MKRYRASIRTTGFTLIELLTALLILSALALMCYRGLGAVLDARD
                     KVERETDKWRRVAGFFGRFERDVLLAAPRAVRTSSGSAAAWLGRPAGTPEPVVEFSRF
                     ASSGGTDTPRRLGYRLNDNRQIELWLWPGLDVATDASAARYPVLEDVSAFEVQYLNAD
                     LVWSYVWPESRLSQPLPRAVRLRVLLGSGEELVRVFALIT"
     gene            5877..6788
                     /gene="gspK"
                     /locus_tag="VN496_05980"
     CDS             5877..6788
                     /gene="gspK"
                     /locus_tag="VN496_05980"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HXF16531.1"
                     /translation="MRVAERGVAIVLAMGVVAMAAMAATAIMITQSTWSRATELTADH
                     VQGQLLIQAGADWARALLAYDRRSNNVDHLGEPWAMRLPAMPVENGELTGYIEDQHSH
                     FNVNNIVKDGKLNVAQYEIFKRLLAILKLPPALADTLVDWVDADNETQPQGAEDAYYL
                     ALDPPYLAANRPLLDVDELALVSGFNENVRARLRPFVTALPRFTAVNPNTATPEVLAA
                     VIDGLGLDGARNMVARRERAYFRSFEDFYKQLPRGLNVPSENITVSSDYFVATLRVTM
                     GGAQARGTALLARTTDFWPAIVWTKTQ"
     gene            6785..7993
                     /gene="gspL"
                     /locus_tag="VN496_05985"
     CDS             6785..7993
                     /gene="gspL"
                     /locus_tag="VN496_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HXF16532.1"
                     /translation="MSLLRIYGSLAEAPQRCQWVFASNGAEPVAGEGRLTELPRRAER
                     IQFVVPAAQVLITRAQVPQEAKRRGGAVLAFAVEEQTAGEPDANQVNWLGAVGDNDVL
                     AVVDKSGVRRWREALDAVGIRNYEVHCETLLLPWVATQWSLAWNGTDGFVRTGEFEGA
                     ATDCGDRNTPPLSLRLMLENVEAHGAGPSSIALYTTAPEAAPDLEAWTRELGIAVSHA
                     GNWDWRTAKDNGVTLAQERAGWRAFSGTVAKLRPAAWILGAAFAIHAGALLLDWTSLA
                     HEQRKLRQQMDTRFRAAFPDAVAVVDPALQMRRKLAEARHAAGQPDSGDFLPMIQKVT
                     AALKDLPAGALRIISYESGRMTLEVASKDAAILQRIATRLAQSGLTVDPSTAPVRAGS
                     PTVVFTVRAS"
     gene            7990..8481
                     /locus_tag="VN496_05990"
     CDS             7990..8481
                     /locus_tag="VN496_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HXF16533.1"
                     /translation="MKAQLLKLWESRSPRERTIIAAAGVVLGIFIYVALVQTATRARI
                     QLRSSVLSLRAQANSLEQQAVELERVRAAPPLLASQTDLRTLVQSEAAAAGLSRSLVS
                     IDALDANQVAVVFGAVAFADWHNWIVALNLQQVRVDACRIEAMSTPGLVSITATLARA
                     KPK"
     gene            8478..9221
                     /gene="gspN"
                     /locus_tag="VN496_05995"
     CDS             8478..9221
                     /gene="gspN"
                     /locus_tag="VN496_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HXF16534.1"
                     /translation="MNRWLAIAIGLGIYAIALLVTAPATLLDGALARASHGRLRLAQA
                     EGTLWSGTGQIEIRDASGRSGVAKTVAWRVMPQSAWRGRLVCEVTLDQPGKAFPVAVS
                     LSTIEVSNADINVPATVLGLGVPKLGPLGLTGDVLIHIANLSFRRDAMNGTATLQWLA
                     AGSSLTTVAPLGDYELKLDGEGITLHAYLRTLEGPLWLDGQGAWKQGTNPAFLANARI
                     SPEYQQQLSPLLRLIAVERGEGRFELQLN"
     gene            9377..9940
                     /locus_tag="VN496_06000"
     CDS             9377..9940
                     /locus_tag="VN496_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16535.1"
                     /translation="MSQVKDLVPAGYMAKKVSMAPARLAAVATQIYSVSDCLSKPFCQ
                     FISHWQHNGFWFFDSPAVIAAVAREEGVELAAHRMFYYEVHGRQFDEQTESWQPVVVD
                     ASIGTQVVAPSRSRLEGFDVVTFSAGTGPECSPLSCNYLAEAIAVNENCLLTSFDEAR
                     RLLEAGAFAHSEPGPFRIFAVHSCASV"
     gene            9958..10596
                     /locus_tag="VN496_06005"
     CDS             9958..10596
                     /locus_tag="VN496_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXF16536.1"
                     /translation="MYLPAHFAETRAEVLRELIAAHPLGALVTSGPEGLDANHIPFEF
                     DPLPEPLGTLRAHVARANPVWRQFSGDVESLVIFQGPQVYITPSWYATKAETGKVVPT
                     FNYIVVHAYGTVRAIEDEAWLRNFVAKLTERFESTRTNPWSITDAPETFIATNLRAIV
                     GIEIKVTRMIGKWKTSQNRPQADRDGVVAGLRESADPVAQAMAHAVEAWRPK"
     gene            10645..11091
                     /locus_tag="VN496_06010"
     CDS             10645..11091
                     /locus_tag="VN496_06010"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HXF16537.1"
                     /translation="MSKLVRSRHGLAVCSSFVCILSLWAPQPATAAEWVLIASDDESS
                     FYFDMASLKRDANIVEVVLLWDFPELQLTRRPVKPYLSATRLTRFDCKAGRRANVEST
                     LYRGRMARGEITDVYRTPDAEIEFEWVNPDAPGGESMRRVCAASAQ"
     gene            11150..>11515
                     /locus_tag="VN496_06015"
     CDS             11150..>11515
                     /locus_tag="VN496_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF16538.1"
                     /translation="MLQARQIECIRGDRRLFHDLSFQLEAKQALRISGENGSGKTSLL
                     RMVAGLSPPQGGEIRWNDARISVLADDYRRELIFIGHANGLKEELTAVENLQHALTLT
                     GRRIECGELRSALAELGLTA"
BASE COUNT         2209 a         3320 c         3569 g         2417 t
ORIGIN      
        1 ctcaatggga tcttccactg tcatgatatt cagcgtggcg gcatcgagcc gcgacagcgc
       61 tgcgtacagc gtagtggtct tgccggagcc ggttgggccg gttaccagca caataccgtg
      121 cggttcctga atcagccggt ccatatgcgc cagcgttgcg tcgtccatgc ccagacggcc
      181 aagatccagg cgccccgctt gtttatccag cagccgcaac acgacgcgct cgccatgccc
      241 ggtgggaatg gtcgatacgc gcacatctac tggctttccg gctatgcgca aggtgatgcg
      301 tccgtcctgg ggcagacgtt tttccgcgat gtccagctgc gccatgatct ttacccgcga
      361 aacaatcgcg ccatgcagcg cgcgtgcggg ttcaatcagg tcgcgcagcg tgccgtcgat
      421 gcgcagccgc accacggaac gcgtctcaaa gggctcgacg tgaatgtccg aagcgccgtc
      481 ccgcagcgcc tgggtgaaca aggcattgat caagcggatg accggcgcgt cgtcctggct
      541 ttcgagcaaa tcctcgatcc gggggatttc ctgcaacaag cgtgaaagat cgaaatcctg
      601 cgcaagatct cccgccaacg ccaccgcgcc cgcatccgcg ccgttgtatg ttgccgcgat
      661 caattcgtcg aattgctcag gcgcgatgcg gcgtgcattg agcggaacgc caagtgcccg
      721 gcgcagttcc gccagtgcgc cggcatgcgc gtcggtgcgc accgcgacct gtgcgatatc
      781 gccgtccatg gccgtgacca ccacgccctg ggccttggcg aacgtgtacg gaatcctgat
      841 tgcaccccgg ttcatttgcg tccttcgttg ttgtacggct tgacttcaat ttgcagggcg
      901 ggcgtctgct ctctgggcgg cagcaaaggc gattgcacgt ccggaaggac cacatcgtgc
      961 gccggtttgg ctttttgctg ttcgcccagg atgtaatcgt agcgctcgcc ggtgtattcg
     1021 tccgcacgct ggctgctgcg taccagtgtc ggacgcagga acaccatcag attggttttg
     1081 acgtgtgagc gcgtcttgta gcggaacagc gcgcccagga tcggaatatc gccaagcacc
     1141 gggacttttt gcacgccgtc gagcaccgta tcctgaatca atccgccaat caccacaatc
     1201 ctgccgtcgt cgaccaggat cgttgattct accgcgcgct tgttggtaat gacgcccgct
     1261 ggattggtgg cgtcttcgat gctggaaact tcctggtaga tctgcagccg cactgtgccg
     1321 ccttccgaaa tctgcggctt gacgcgcaat gtcacgccca catcatgccg ctccaccgtc
     1381 tggaacggtg tcggcgtggt ggcgcccccc gacagtgcgt actggccggt tacgaagggc
     1441 acattctggc cgatgagaat cacggcttcc tcgttgtcca gcgtcaatag cgtcggcgtg
     1501 gcgaggatat tcgaacttga gtcggtctgc agggcgcgaa ccaggaaacc gaggttcaga
     1561 atctgcccca cacccggcac attgacctgg cccttgatga tgccgacatt aagcccctgg
     1621 ccagccgtac ccgggttttg cgcgatgcca ataatattct ggccggcgcc accgaaattg
     1681 gtcccgccga aaggttgcgc gctgttcttg ttataaccgt tcagatcctg ccactggata
     1741 ccgaactccg cggccttgtt cgcgccgact tcggcgatta gcgcttcgac atagacctgc
     1801 gcgcgacgca catcgagctt atcgagcgcc gcgcgcagat tgttgtagat cgcatcagga
     1861 gcggtgatga taatcgagtt cgtcgatgcg tcagcctgga tgattcccgg cgcggttggg
     1921 gtggtttgcg cctgatttat gctgaaagac gaggcggccc ccactccgga ggtgccctgt
     1981 cccccgagct gggattgaga ttggggtgtc cccatgggcg cattcgcgcc cagcgaggtg
     2041 cgttgcgcag tcggtgttga agaaggggcg gcggcggtgg tgcctccttc tccctggtag
     2101 atggcgcgca ggatttcggc aagtttcacc gcttctgcgt tcttcagata tacaacatga
     2161 atgtttccgc ccgcgttcgt cggtgaatcc agtattgcga tgaacttgcg caagcgtgcc
     2221 agccgtgacg tatcaccgga gcgcgccagc aaactgttcg agcgggcatc cgcaactacg
     2281 gtgaagcgtt gtgtgggctc ggcggcgccc tgagcctggg cggcttcggc aaaaagccgg
     2341 tttacggtgg ccgccacatc gaccgcggac gcatacttga gcggaattac gaccggatcg
     2401 tttgcatttg gctggtctat ggcatcgatg atgtcagcga tgcgttcaag attgtttgca
     2461 taatccgtaa tgaccagcgt gttgttgttc tgatatgcgg taatggtatt gcttggagca
     2521 atcagcgggc gcaggatcgg cagcgcttgc acggccgatt cgtatttcag cgtaaacacc
     2581 ctggtctcga tccggtcgcc gctgatgcct ccggtttcgc cttgcgcttg cgtcggactt
     2641 gaatgcagtt ttgcatcagc ctccgggacg actttgacaa gtccacggtc ctccactgcg
     2701 gcgaatcctt gcaatcggag cgccgccaga aacacgtcgt acacccggct tctcggcatt
     2761 ggcttcgccg atacgatatt gaccgtgcct ttcacgcgtg gatcaataac aaagttcttg
     2821 cccgtaatct cgctgactac cttgactaca ccttcgatgt cggcattgac gaaatttagc
     2881 gtcacgacat ccggcgcttc acgcggggga cggcttggtg ccgcggggct tggtggcggc
     2941 gcttccgcag gcatgccttt taccgccggc gtagcagtgc gcgcggccgg ctttgagtcg
     3001 gccggccggg ccggcacctt ggccgcatcc ttgggacgct cttgcgaatg cgccagcggg
     3061 actacgcaaa tagcaagcga ggccaccagc agcagaagca cactcggctt ccgactcttt
     3121 cgcgtaatga ggatcatttt tcgggcgaac gatcggcaaa cagcgcacgg catgaaaaaa
     3181 ttcacctgtt cgtccgtacc ggtgccggct ccgcgggctt gcttttctcc ggccatgtaa
     3241 gcgtttcctg tctcccgttt cgctccagcg tgacatgatc ggcttcaact tcggcgaggc
     3301 ggatgccggg agctacgtcg tcgccttcgt gcaccgccag aatctgccgt ggctcgactt
     3361 ccattaccgc gtatccttgc cttccggccg tggcggctac gatgcccaaa agccggattg
     3421 caattccggt tggcgcagcg gcattgcgct cctttgcagc ggcaccaaat aaggtgccca
     3481 ccgccgcgcc ggcactgttc cccgcgccgg cttgcgcccg ccattccgcg cgtggcgcaa
     3541 accaggtcca ggtccaatat gcggcaacca ggctaaatgc cgtcagcgca gcgatcgtga
     3601 ccaccgccac cagcgctcgt tgggcgagat tggatcggtc gaacggcaat tgcatggcta
     3661 acattccgcg gtgaaaccgg ttcatgtcag ttattttgtt gacggaaaaa aaccttagga
     3721 cgatctaagg ttagtatgga aacgcccgga cagtatgtgc gggagcgaac agagtggcag
     3781 tcagcaaatg ggataattat ggccatccat ctgaaacggg acgaatgcga acgtccctga
     3841 gatcaatata ttacatgaag gaagcctaaa tgagatgcct aaagggccaa cgcgggttca
     3901 ctttgctcga agtcatggta gtggttgtga tcctcggtat tcttgcggcc ctggtcgtgc
     3961 ccaaaattat tagcaggcct gacgaagcgc gcataattgc cgccaaacag gatatcgcca
     4021 gcctgctgca ggcattaaag ctttaccgtc tggataatca tcgttaccca tccagcgaac
     4081 agggattgca ggctcttgtg gtgcaaccga ccggcacgcc ggtacccccg aattggaaag
     4141 cgggcggcta tatcgagcgg ctgccaaaag acccttgggg aaatgcctac caatatctga
     4201 atcccggagt gcatggcgaa atagatgtat tcagtctggg tgccgacggc gcaccgggcg
     4261 gcgaaggcaa cgacgccgat atcggttcct ggtcgctgta gccttgaagc agccgtcgcg
     4321 caaccggtga tcatttcatc gccgcgtgtg ctgcgattta atcggttctt ctccgttccg
     4381 catctgtgaa cctgcatgcc cgtctccctg cgccaatcgg gctttacgct gatcgaaatg
     4441 ctggtggtgc ttttgatcat gggtattttt gtcggcctgg tgagtaccat tacgcaaccc
     4501 gacgaccacg ccatgctcaa gttggaagcg cagcgactgg cacgtttgct ggatcttgcc
     4561 gccaccaagt cgcgcctgac tggcaggcct accgcatgga cggcggacgc acacagttac
     4621 cggttctggc aattcagcga agatgctttc tggactgaaa ttctggatga cgataccttg
     4681 cgcgcacggg tactaccgca ggatatggcg atcttgcgcc tggaagtcga gaaccggaaa
     4741 tcaacagatc agatgcgact ggaattcact ccatacgggc aacccgtttc attcagcttg
     4801 cagatgggcc tcggtgatgc acgggagacg gtagtggggt cgccaattgg cgaggtatcc
     4861 gttttgtcca gacaggaagc gaccaatgag cccacggtgc accgctagga ggaccggttt
     4921 cacgctggtc gaagtgctgg ttgcgttggc gattgtttcg atcgccctgc tcgcagcact
     4981 gcgggtcgcg ggcggaggca ccaacagcgt cggagacctg cgtgcccgcc tgcttgctga
     5041 gtgggtcgcg caaaatgtac tggccgaaca gcgcgcgcgt gcggagtggc cgtcgcttgg
     5101 gatcctgcgc ggtacgcaat tgcagggcgg cgtccaattt gcgtggcgcg aggaagtgat
     5161 cgccacgccg aatgtcatgt ttcggcgtgt cgacattcag gttttcgcta ccgcggagga
     5221 tgcccattct ctggcgcacc tcaccggctt cgtggtcaat gcgccggtgc caaatcgatg
     5281 aagcgttatc gtgcaagcat ccggaccacc ggctttacgt tgatcgaact gctgacggca
     5341 ttgctcattc tgtctgcttt ggcgctgatg tgttaccgcg gactgggcgc ggtgctcgac
     5401 gcacgggaca aggtcgagcg ggaaacggac aagtggcggc gcgtcgccgg tttctttggg
     5461 cggttcgagc gcgacgttct gcttgccgcc ccgcgtgcgg tacgcacgag ttccggtagt
     5521 gccgcagcct ggctgggtcg acctgccggg actccggagc ctgtcgtcga attcagccgt
     5581 ttcgcttcca gcggggggac cgatacgccg cgacgactcg gctaccgcct gaacgataat
     5641 cggcaaatag agttatggct gtggccgggc ctggatgttg ccaccgatgc gtcggcggcg
     5701 cgttacccgg tacttgaaga cgtgagcgct tttgaggtgc aatacctcaa tgcggacctc
     5761 gtttggtcgt acgtctggcc ggagtcacgc ctgtcccagc ccttgccgcg cgcggtacga
     5821 ttgcgcgttt tgctcggttc cggggaagag ctggtacgcg tgttcgcgct gattacatga
     5881 gagttgcgga acgcggcgta gccattgtgc tggcgatggg cgtggtcgcg atggctgcaa
     5941 tggcggctac cgcaatcatg atcacgcaaa gtacctggtc gcgtgcgacg gaacttactg
     6001 ctgaccatgt gcagggacag ttgctgatcc aggccggggc ggattgggcg cgtgcgctgc
     6061 tggcttacga tcgtcgcagc aacaacgtgg atcacctggg tgaaccctgg gccatgaggc
     6121 tgccggccat gccggtcgaa aatggcgagc tgaccggcta cattgaagac cagcacagcc
     6181 attttaatgt caacaatatc gtcaaggacg gaaaactgaa tgtcgcgcag tacgagatct
     6241 tcaagcgttt gctggcaatt ttgaagctgc ctccggcgct cgccgacacg ctcgtggatt
     6301 gggtggacgc cgacaacgaa acgcaaccgc agggcgcgga agatgcttat tatctggcgc
     6361 tggacccgcc ttaccttgcg gccaatcgtc cgttgctcga tgtggatgag cttgcactgg
     6421 tgagtggttt caatgagaac gtgcgcgcca gactgcgccc gtttgtcacc gcgctgcccc
     6481 ggtttacggc agtgaacccg aataccgcta ctcccgaggt gctcgcggcg gtgatcgacg
     6541 gccttggcct cgacggagct cgcaatatgg tcgcccggcg tgagcgcgct tatttccgca
     6601 gtttcgagga tttttataaa cagcttcccc gtggactcaa tgtacctagt gaaaatatca
     6661 cggtcagcag cgattacttc gttgcgacgt tgcgcgtgac gatgggcggc gcgcaagcac
     6721 gcggtactgc attgctggcg cgaaccaccg acttctggcc tgccatcgtg tggacgaaaa
     6781 cgcaatgagc ctgctgcgca tttacggctc gctcgcagag gcaccgcaac ggtgtcaatg
     6841 ggtatttgcc agcaacggag ctgaaccggt tgccggagaa ggaaggctca cggaattacc
     6901 acggcgggca gagcgcatcc agttcgtggt tccagcggca caagtactca ttacgcgcgc
     6961 ccaggtgccg caggaagcca agcgccgcgg cggagccgtg ctcgcgttcg cagtcgagga
     7021 acagactgcc ggcgagcccg atgcgaacca ggtcaattgg ctaggcgcgg ttggcgacaa
     7081 cgatgtgctc gcggtggtcg acaagtccgg ggtgcggcgt tggcgtgagg cgctcgacgc
     7141 cgtaggcatc cgtaactacg aagttcactg cgagaccttg ttgttgccgt gggtggcgac
     7201 ccagtggagt ctcgcctgga atggcaccga tggcttcgta cgaaccggcg agtttgaagg
     7261 cgcagcaact gattgtggcg accggaacac gccgccgtta tcacttcgtc tgatgctgga
     7321 aaacgtggaa gcgcacggcg cgggaccgag ctccattgcg ctttacacga cggcacccga
     7381 agcggcgccc gatctagaag cgtggacgcg tgagctcggc atagcggtgt cccatgcggg
     7441 aaattgggac tggcgcaccg ccaaggataa cggcgtgacc ctggcacaag agcgggcagg
     7501 ctggcgcgca ttctccggaa ccgtcgcaaa gctgcgacct gcagcctgga tactcggtgc
     7561 ggctttcgcg attcacgccg gtgcgttgct cctggattgg acatcgctgg cgcacgaaca
     7621 acgcaagctg cgccagcaaa tggatacgcg atttcgcgct gcatttcccg atgccgtggc
     7681 cgttgtggac cccgcgctcc aaatgcgccg caagctcgcc gaagccagac atgcagcagg
     7741 ccaaccggac agcggcgatt ttctgccgat gatccagaaa gtcaccgccg cactgaagga
     7801 cttgccggca ggcgccttgc gcatcatctc gtatgaaagc ggccgcatga ctctggaagt
     7861 cgcgtccaag gacgcggcga tactacagcg cattgccaca cgcctcgctc aatctggtct
     7921 gacggtcgac ccgtcgaccg ctccggtccg ggcgggaagc ccgacggtag tattcacggt
     7981 gcgcgcctca tgaaagccca gttgctgaag ctttgggaat cacggtcacc acgtgagcgc
     8041 acgatcattg ccgcggcggg cgtggtactg ggcatattta tttatgtagc gctggtacag
     8101 actgcaacac gggcgcgcat tcaattgcgt agcagtgttt tgtccctgcg cgcgcaggct
     8161 aacagcctcg aacagcaagc ggttgaactt gagcgcgtgc gcgccgcacc gccgctcctg
     8221 gcatcgcaaa cggacttgcg cacgctggtt cagtccgaag cggcggcggc cgggttgtcg
     8281 cggtcgctgg ttagcatcga tgccctggac gcaaatcaag tggccgtggt attcggcgca
     8341 gtagcattcg cagattggca caactggatc gttgccctga atctgcaaca ggtcagggtt
     8401 gatgcttgtc gcattgaagc catgtcgacg ccgggtctgg tgagcataac tgccacgcta
     8461 gctcgcgcga aaccgaaatg aaccggtggc tcgcgattgc aatcgggctt ggtatctatg
     8521 cgattgcact cttggtcaca gcgccagcta cgctgctgga cggtgcgctc gcgcgcgcga
     8581 gccatggccg gttgcggttg gcgcaggccg agggcacgct gtggtctggg accgggcaaa
     8641 tcgagattcg agatgcaagc ggacgcagtg gtgtggccaa gaccgtcgcc tggcgtgtca
     8701 tgccgcaatc agcatggcgc ggtcgcctgg tttgcgaagt gacgttggac cagcccggta
     8761 aagcgtttcc ggtagcggtc tcgttgtcga cgattgaggt ttcgaatgcc gatatcaatg
     8821 ttccggccac tgtgctcggg ctgggcgtgc ccaagctggg gccgcttggc ttgaccggcg
     8881 acgtactgat tcacattgcc aatctgtcgt tccggcgcga cgccatgaac ggtaccgcca
     8941 ctttgcaatg gcttgccgcc ggatcctcgc tgacgacagt tgcgccgctc ggtgactatg
     9001 aactcaagct agacggtgaa ggtataacgt tgcacgctta tctgcgcacc ctcgaagggc
     9061 ccctttggct cgatggccag ggcgcgtgga agcaaggcac caatccggcc tttctggcca
     9121 atgcgcgcat cagtcccgag taccagcagc agctctcgcc cctgctgcgc cttattgcgg
     9181 ttgaacgggg cgaggggcgc ttcgaattgc aactgaactg aaccaatatc cggcgcggag
     9241 caatgcactt gcgaagcgga ccgaatagca ggtttgatct gcgacaaccg tatgcaatgc
     9301 gtgtactgaa cttcatcctg agcgacttac gagcatccat ttgcggactg caatctctcg
     9361 gtagttcgca aaatcgatga gccaagttaa agacctggtt ccggccggct acatggccaa
     9421 aaaagtatcc atggcgccag cgcggctcgc ggcggtcgcc acgcaaatct attcggtcag
     9481 cgactgccta tcgaaaccgt tttgccaatt catcagccat tggcagcata acggattctg
     9541 gttctttgac agtccggccg tgattgctgc agtggcgcgc gaggaaggcg tcgaactcgc
     9601 ggcacacagg atgttttatt acgaagtcca tggtcggcag tttgatgaac agaccgaaag
     9661 ctggcagccc gtggtagtgg acgcttctat cggaacgcag gttgtggcac cgtcgcgcag
     9721 ccgtttggaa ggattcgatg tggtaacgtt ttccgccggc accggtccgg aatgttcgcc
     9781 gttatcctgc aactacctgg cagaggctat tgccgtgaac gagaattgcc tcctgactag
     9841 cttcgacgag gccaggcgcc ttctcgaagc aggtgcattc gcgcattcgg agcccggtcc
     9901 atttcgcatt tttgcagtac attcatgtgc gtcggtataa cgtgatttgg tgacttgatg
     9961 tatctgccag cgcattttgc agaaacgcgc gcggaagtcc tgcgtgaatt gattgccgcc
    10021 catccgcttg gggcattggt tacttccggg ccggagggcc tggatgcgaa tcacatccct
    10081 tttgagttcg atccgcttcc cgagccttta ggcacgttgc gcgcgcatgt tgcgcgcgcc
    10141 aatcctgtct ggcgacaatt ttccggggat gtggaatcgc tggttatctt tcaagggccc
    10201 caggtttata tcaccccttc ctggtacgcg acgaaggcgg aaacagggaa ggttgtgcca
    10261 actttcaact acatcgtcgt gcatgcgtac ggaaccgtgc gcgcgatcga agacgaagcg
    10321 tggcttcgca acttcgtggc caaactgacc gagcgcttcg agtcaacgcg caccaacccc
    10381 tggtcgataa cggacgcacc ggaaaccttc attgcgacga acttgcgcgc gatcgtggga
    10441 attgaaataa aagtgacccg tatgatcggc aaatggaaaa cgagccagaa tcgtccgcag
    10501 gccgaccgcg acggggtagt ggccggcttg cgcgagtcgg cggaccctgt tgcccaagcg
    10561 atggcgcacg cagtggaagc gtggcggcca aaatgaaaca gacgcagcag cgcggccaaa
    10621 gtaggttggc atgagtaaag tggcatgagt aaactggtcc gttcccggca tggcttggca
    10681 gtttgttctt cgttcgtctg catcctctcg ctttgggccc cgcaaccggc cactgccgcc
    10741 gagtgggtgt tgatcgcgag cgatgacgag tcatctttct atttcgacat ggccagcctg
    10801 aagcgtgacg cgaatattgt cgaagtggtt ttgctttggg attttccgga gttgcaactg
    10861 acccgcaggc cggtgaaacc ctatctttcg gcgacccggt tgacgcgttt cgattgcaag
    10921 gccggccggc gtgccaatgt ggaatccacg ctctatcgtg ggcgcatggc aaggggtgaa
    10981 atcaccgatg tttatcgcac gccggatgcc gaaatcgaat tcgagtgggt caatccggac
    11041 gcgccgggcg gggagagcat gcgccgcgtg tgcgccgcga gcgcgcagtg atcgatgggc
    11101 catgcacggc tcagctataa tggcgcgccc gcgatagtcc cggatgccaa tgcttcaggc
    11161 acgacagatt gaatgtatcc gcggcgaccg gcgactattc catgatttgt cgttccaact
    11221 ggaagcgaag caggcgctgc gtatcagtgg agaaaacggc agtggcaaga ccagcctgct
    11281 gagaatggtg gccggcctgt cgcccccgca aggcggggaa atccgttgga acgacgcaag
    11341 aatatcggtg ctggcggacg attaccgacg tgaactgata ttcatcgggc acgccaacgg
    11401 cttgaaggag gagttgaccg cggtcgaaaa tctgcagcac gcgctcaccc ttacgggccg
    11461 gcgcattgaa tgcggcgagt tgcgtagcgc gctggcggaa ttgggactta cggcc
//
LOCUS       DATMZQ010000186         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_145517, whole genome shotgun sequence.
ACCESSION   DATMZQ010000186 DATMZQ010000000
VERSION     DATMZQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145517"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VN496_06020"
     CDS             <1..906
                     /locus_tag="VN496_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXF16539.1"
                     /translation="GVFNVIHGDKVAVDALLNDTRIAAVSFVGSTPIAEYIYRTGTAS
                     GKRVQALGGAKNHLVVMPDADLEQAVDALIGAAYGSAGERCMAISVAVAVGTVADKLV
                     EKLAERAKALKIDTGTSDAAEMGPLVTRQHLDKVRGYVDLGVKEGAKLVVDGRGLKVK
                     GYENGFFLGGCLFDQVKPDMTIYREEVFGPVLAITRVPDFEAAVELVNKHEFGNGVSL
                     FTRDGDCAREFAMRIQIGMVGINVPIPVPMAFHSFGGWKRSLFGDHYAYGPEGVRFYT
                     HLKTVTTRWPTGIRKGAEFIMPTMK"
     gene            931..1173
                     /locus_tag="VN496_06025"
     CDS             931..1173
                     /locus_tag="VN496_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16540.1"
                     /translation="MRLLIAIILAGAMATACDKSKPPTPKSEAVSDTHPVKNVETLPS
                     GNIQEGARTMNRASDLGKDLEQQKQDRDKDGESQNK"
     gene            1185..1796
                     /locus_tag="VN496_06030"
     CDS             1185..1796
                     /locus_tag="VN496_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HXF16541.1"
                     /translation="MAKIGTAQALRQLIGAPSALVPHKIHPELNARAIAFIARSPLLM
                     LSTVDEQGQPSVSPKGDPAGFVQVQDNRTLLIPERKGNKLIFSLTNILFNPHVGLIFL
                     VPGTSETLRLQGTAELLDDAELCGKFPARGRDALLVMRVRVTQCYFHCAKAFLRSELW
                     DPATWQQKMTISFGEEIAESGGMGVDQIKEFDQAVAGRYKSDL"
     gene            1893..>2395
                     /locus_tag="VN496_06035"
     CDS             1893..>2395
                     /locus_tag="VN496_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16542.1"
                     /translation="MNRLLNTLAIALLVTLAACATPKKPEPEIPKAVPTPPAPIFIHD
                     RSVIPGERAGPIELGMSLRKLVEVFGEPVSSSSTRIPSGRSALLYRYADPDASPDGAL
                     LVMVRENDNTVYSVQIDRIESFKTREGVHYGSSEALVRAGLGRPQSIQESATNEMPRE
                     YCYISGLG"
BASE COUNT          545 a          678 c          708 g          464 t
ORIGIN      
        1 ggcgtgttca acgtcatcca cggcgacaag gttgccgtcg atgcgctgct gaacgacacg
       61 cgtatagcgg cggtgagttt cgtcggctcc accccgatcg cggaatacat ctaccgcacc
      121 ggcaccgcgt cgggcaaacg agtgcaggcg ctcggtggcg caaagaacca tctggtcgtg
      181 atgccggacg ctgatctgga gcaagccgtg gatgcgctca tcggcgcggc ttacggttcc
      241 gcgggtgagc gatgcatggc gatttcggtt gccgtcgcag tcggaactgt cgccgacaaa
      301 ctggtggaaa aactggccga acgggccaag gcgttgaaga ttgacacggg gaccagtgac
      361 gcagccgaga tggggccatt ggtcacgcgc caacatctcg acaaagtacg cggttacgtc
      421 gatctcggcg tcaaggaagg cgcgaaactg gtcgttgatg gtcgcggact caaagtcaaa
      481 ggctacgaga acggcttctt cctcggcggt tgcctgttcg atcaggtcaa gcctgacatg
      541 acgatctacc gtgaagaagt attcggtccg gtattggcga tcacgcgcgt gcccgatttc
      601 gaagctgccg tggagctcgt caacaagcac gagttcggca atggagtttc gttattcacg
      661 cgcgatggcg actgcgcgcg ggaattcgcc atgcgtatcc agatcggcat ggtcggcatc
      721 aacgtgccga tcccggtgcc catggcgttc cacagttttg gcggctggaa acgctcgctg
      781 ttcggcgacc attacgccta cggcccggag ggcgtacggt tctatacaca tttgaaaact
      841 gtgaccacgc gctggccaac cggcatccgc aaaggtgcgg agttcatcat gccgacgatg
      901 aaatagcttc agcacataga ggaaacaatc atgcgtttat tgattgcaat catcctggcc
      961 ggcgcgatgg caactgcttg cgacaaaagc aagccaccga cacccaaatc ggaagccgtc
     1021 tcggataccc atcccgttaa gaatgtcgag acgctgccgt ccggaaacat tcaggaaggc
     1081 gcgcggacca tgaatcgggc atccgatctg ggcaaggatc tggagcaaca gaaacaggac
     1141 cgcgacaagg atggcgagtc gcagaataaa tagccaagcg actcatggcg aaaatcggca
     1201 cggctcaggc actacgacaa ctcattggag cgcccagtgc gctggttccg cacaagattc
     1261 atcctgagct gaacgcccgc gccatcgcat tcatcgcccg atcgccgttg ctgatgctgt
     1321 ccacggtgga tgagcaaggc caacccagcg tgtctcccaa gggggatcca gcgggattcg
     1381 tgcaagtcca ggacaaccga accttgctga taccggaacg caaaggcaac aagctgattt
     1441 tttcgctgac caatattctg ttcaatccgc atgtcggatt gatattcctg gttcccggga
     1501 ccagcgaaac actacgactt cagggcacag cagaattgct cgacgatgcc gagttatgtg
     1561 gaaaatttcc ggcacgcggg cgcgatgcac tgctggtcat gcgggtgcgc gttacgcaat
     1621 gctacttcca ctgtgccaag gcgttcctgc gcagcgagtt atgggatcct gcaacgtggc
     1681 agcaaaagat gacgatctcg ttcggcgaag agatcgccga aagcggcggc atgggtgtcg
     1741 atcaaatcaa ggaattcgat caagctgtcg cgggccgcta taagtccgac ttgtgaaatc
     1801 gaacgacctg cctgacactt ccgcacgctg cacggtgcca gcacgccggg cagtgtccac
     1861 aggatttcgt cgtcttaacc tcggaccaca acatgaaccg actgcttaat accctggcga
     1921 ttgcattact ggtcacgtta gcggcttgcg ccacacccaa aaaacccgag ccggaaatac
     1981 cgaaggcagt cccaacgccg ccggcgccga tattcattca cgacagatcc gtgattcctg
     2041 gtgagcgggc cggaccgatc gagctcggga tgtcgctgcg aaagctggtc gaggtttttg
     2101 gggaaccggt atccagcagc tctacccgca ttcccagcgg ccgtagcgca ctgctgtatc
     2161 gctatgcgga cccggatgct tcaccggatg gcgcgttact ggtgatggtg agggaaaacg
     2221 ataatacggt ttactcggtt cagatcgacc ggatcgaaag ctttaaaacg cgcgagggcg
     2281 tgcactacgg ctcttccgaa gcgctggtgc gcgccggtct tggaagacct caatccatcc
     2341 aggagtccgc taccaatgaa atgccccgcg agtattgcta tatcagcgga cttgg
//
LOCUS       DATMZQ010000187         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1182131, whole genome shotgun sequence.
ACCESSION   DATMZQ010000187 DATMZQ010000000
VERSION     DATMZQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182131"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VN496_06040"
     CDS             complement(<1..2078)
                     /locus_tag="VN496_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXF16543.1"
                     /translation="MVTDPVCGMHVDEKSAAASATFSGQRYYFCCSGCRDKFVADPAS
                     FLDKTEADATSADRIYRCPMHPDVQQHAPGKCSKCGMALEAKHAPKAPSLPLQDSTSA
                     APGAQYTCPMHPQIVRDAPGDCPICGMALVPIAGTGDADDAELNDLKWRLWAGIALSV
                     PLIVLAMSPMIGIREPFGVMPRQTGWIQFVLGTPVVLWAGWPILRKFWLSLAHRALNM
                     YTLIGLGVGFAYLFSLAAIFVPGWFPQEFREHDGAVGTYFEAAAVIVTLVLLGDYLQL
                     RAMGQTSKAIEQLLHLAPNQALRLRADGTEHAVPLEQVAVGDRLRVRPGDKVPVDGIV
                     LEGSSRVDESMITGEPVPVQKLPGANVTGATVNGNGSLVIRAERVGSDTLLARIVHMV
                     GEAQRTRAPIQRLADLIAAYFVEAVVAIAVVTAMTWWWVGPDPRFAYAFLNAIAVLII
                     ACPCALGLATPISMTVAMGQGAKAGILFRNAEAIERMRDIDTLVVDKTGTLTLGHPAM
                     TGFIAEGIAENEALALVAGVEQLSEHPIGLAIVDGAKARSITPPAATNFAAENGLGVQ
                     AEIQGKRVLVGSRAFLNQRGVLTQSWEARAEAWRAEAKTVVFFAIDGMAAGIAAVADP
                     IKDSTPEAIATLKRAGIRVVMLTGDSRSTAEVVARQLGIDEALAEVLPEDKAGHVKRL
                     QEEGRNVAMAG"
BASE COUNT          432 a          751 c          661 g          433 t
ORIGIN      
        1 cccgccatgg ccacgttgcg gccctcctcc tgcagccgct tcacatggcc ggccttgtcc
       61 tcgggcaaca cttcagcaag cgcttcatcg atgcccagct ggcgtgcaac gacttcggcc
      121 gtgctgcgcg aatcgccggt aagcatcacc acacgaatgc cggctcgctt cagagtggcg
      181 atggcttccg gcgtgctgtc cttgatcgga tcagcgaccg ccgcaattcc ggcggccatg
      241 ccatcgatgg cgaaaaacac cacggtcttt gcttccgcgc gccatgcctc tgcccgcgcc
      301 tcccatgact gtgttagcac gccgcgctga ttgagaaagg cgcgactgcc aaccagcacg
      361 cgcttgccct ggatttccgc ctgcacgccc agcccgttct cggctgcgaa gttcgtggcc
      421 gcgggcggcg tgatcgagcg tgcttttgcg ccatccacaa ttgccaggcc aatgggatgc
      481 tcggacagtt gttctacgcc agccactaac gccagcgctt cgttttccgc gataccctcg
      541 gcgatgaatc ccgtcatggc cggatgccct agcgtcagcg ttccggtttt gtccaccacc
      601 agcgtgtcga tgtcacgcat acgctcgatg gcctcggcat tgcggaacaa aattccggct
      661 ttcgcgccct ggcccatggc caccgtcatc gagattggcg tcgccagtcc aagggcgcac
      721 gggcacgcaa tgatcaatac ggcgattgca ttcaggaatg cataggcaaa tctgggatcg
      781 gggccaaccc accaccaggt catggcggtt acaacggcaa ttgccaccac ggcctccacg
      841 aagtacgcgg caatgagatc ggcaagccgc tggatcggcg cgcgcgtgcg ttgcgcttcc
      901 ccgaccatgt ggacaatgcg ggccagcaat gtgtcggacc caacccgctc tgcacgaatc
      961 accagggaac cgtttccatt cacggttgca ccggtaacgt tggcacctgg caatttctgc
     1021 accggaaccg gttctccagt aatcatggat tcgtccaccc tgctgctacc ctccagcacg
     1081 atgccatcga ccggcacttt atcgccgggc ctgacgcgca gacggtcgcc taccgcgacc
     1141 tgctccagtg gcacggcgtg ttccgtacca tcggcgcgca ggcgcaaggc ctggttgggc
     1201 gccaagtgca gcagttgctc gatcgccttg ctggtctgcc ccatggcgcg caactgcaaa
     1261 taatcaccga gtaagaccag cgtcacgatg acggcagcgg cctcgaaata ggtgcccact
     1321 gcgccgtcat gctcgcgaaa ttcctgcgga aaccaaccgg gcacaaatat cgcagcgagg
     1381 ctgaacaaat aggcaaaccc gacaccgagc ccgatcaacg tgtacatgtt cagcgcgcgg
     1441 tgtgcgagcg acagccagaa cttgcgcaga atcggccaac cggcccacag cacaaccggt
     1501 gtgccgagca caaactggat ccagccggtt tgccgtggca tgacgccaaa cggctcgcgg
     1561 atgccaatca tcggtgacat cgcgagcaca atcagcggga ccgaaagcgc gattccggcc
     1621 cacaaacgcc acttcaggtc gtttagttcg gcatcatccg cgtctcccgt gccggcgatg
     1681 ggcaccagcg ccatgccgca aatgggacag tcgcccgggg catcgcgtac gatctgcgga
     1741 tgcattggac aggtatattg cgcgccaggt gcggctgaag tgctgtcttg caggggcagt
     1801 gaaggggcct tcggtgcgtg cttcgcttcc agcgccatgc cgcacttgga acatttgcct
     1861 ggcgcatgct gctgtacgtc cgggtgcata ggacatcggt aaatcctgtc cgcggatgtt
     1921 gcgtcagctt cggtcttgtc tagaaaactg gccgggtcgg ccacgaactt gtcgcggcaa
     1981 ccgctgcagc agaagtaata gcgctgcccg ctgaaggtcg cgctggcagc agccgatttc
     2041 tcgtcgacgt gcatgccgca tactggatca gtcaccatgg tgtctccgtt cttcctgact
     2101 gaagagactg cgcccccaga gctggcgttc cgcttcagca aattttgaat cagtgctcat
     2161 gcggcccgtt acgcctgacc ctcagcgcct caccagaccc agagccttgc gcttggtcgg
     2221 cgggagcgtg ctgtgaggca ggtgttgacc ccacccactc gtgggcgaca gttccgt
//
LOCUS       DATMZQ010000188         4060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2909181, whole genome shotgun sequence.
ACCESSION   DATMZQ010000188 DATMZQ010000000
VERSION     DATMZQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909181"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VN496_06045"
     CDS             <1..601
                     /locus_tag="VN496_06045"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXF16544.1"
                     /translation="KNGKPAGLADGGTYWHSDYSYLDIPARATLLYSVQVPKIGGDTL
                     FADQQQAYADLPDATKRRLDGLVTLNVYGNRDDLDPQSRTAAAPPTQEQKQKRGAFLI
                     RHPLVRRHPYSARKALYAVSGTSFGIEGMPDADGIALLRELAAHSTQPKYQYRVQYGV
                     GDVVVWDNASVLHSATLTDPLYPRTLLRVTTKEAQRPSA"
     gene            622..1692
                     /locus_tag="VN496_06050"
     CDS             622..1692
                     /locus_tag="VN496_06050"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXF16545.1"
                     /translation="MVTAARSVVLFFLFSCAALAAPYPDPKHGEVELHDFDFADGEIL
                     PSLKLHYFTLGTPARNAEGQIINAVLLLHGTGGQGTGFLTPAFAGELFGVGQPLDANR
                     WFIIVPDNIGHGRSSKPSDGLRAKFPHYGYRDMVAAQRRLLSEGLGVEHLRLIIGTSM
                     GCMHGWMWAGNYPQSTDALLPIACLPAPVAGRNRMERRLIVDAIRNDPEWQGGDYAAQ
                     PQGLITALRMIAISAGSTRQLYQQAPTVEATDRLLDRMVQQRLRTADANDLLYAWDAS
                     RDYDPGENLARISAHVTAVNFEDDERNPPQLQVMERQVARVPHGTYVLIPASDRTRGH
                     ASLADAVLWKEYLVNLLARISR"
     gene            complement(1697..2380)
                     /locus_tag="VN496_06055"
     CDS             complement(1697..2380)
                     /locus_tag="VN496_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXF16546.1"
                     /translation="MMPGEELSVERQRDLMDIGAAFARIRPPAPDVPMPAKPTLADIF
                     NLRLNTPKFGLPTAAAPHCVQTAMQAMKAGMDEETILACLLHDIGLAVVRPDHGWWGA
                     QLVEPYVSEKVSWAIRYHQALRYYPDASVGYEYPQMYITMFGEDYRPPEYIAQAHDYA
                     RKHKWYMISRNITKCDDYAFDKSQPWSIEPFTDIIGRHFRQPKEGLGNDSSATAHMWR
                     TLIDPSKPL"
     gene            complement(2389..3180)
                     /locus_tag="VN496_06060"
     CDS             complement(2389..3180)
                     /locus_tag="VN496_06060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16547.1"
                     /translation="MDLKLQDKSALISGSTKGIGFATAALLAAEGAKVIVNGRTQAAV
                     DAALERIRAVTPRAALDGFAGDLSAAGPTDALIRRFPTVDILVNNLGIFERKAFEDID
                     DAEWQRFFDINVMSGVRLSRAYLPGMKQRNWGRIVFISSESGIQIPDNMIHYGMTKTA
                     QLAISRGLAETCVGTNVTVNAVLPGPTLSDGNKAAIAKRAAGRPFAEFEKEFFEKVRP
                     SSLLKRYATTQEVATMVAYVCSPLSSATNGAALRVDGGVVRACFG"
     gene            complement(3250..3513)
                     /locus_tag="VN496_06065"
     CDS             complement(3250..3513)
                     /locus_tag="VN496_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16548.1"
                     /translation="MTRGRTHKLNGVARPIKTKKDYSAAASVIEKIGDKPDRETIAEK
                     RLQSLLKAMEKFDDETDESDAGNADPDYAGPLRRWSDEPGDTE"
     gene            complement(3708..>4060)
                     /locus_tag="VN496_06070"
     CDS             complement(3708..>4060)
                     /locus_tag="VN496_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16549.1"
                     /translation="SAKFTAHYPDGSDQILLSVPKYDFSWQPLYALDPPKLIPAGTRI
                     VLDLTWDNSPQNPANPDPNQVVRWGDQTWEEMNVGWFRYRAADDNDRAMAASRGGTKH
                     EAAKRDPYDGPRNN"
BASE COUNT          780 a         1240 c         1179 g          861 t
ORIGIN      
        1 aaaaaacggc aagccggctg gattggcgga tggcggtact tattggcaca gcgattattc
       61 ctacctcgac atcccggcgc gggcgacgct gctgtactcg gtacaagtgc ccaagatcgg
      121 tggcgatacc ttatttgcgg atcagcagca ggcctacgct gacctgccgg acgcgacgaa
      181 gagacgtctc gatggactgg tcaccctgaa cgtttacggc aaccgcgatg atctcgatcc
      241 gcaatcccgt actgcagccg caccaccgac gcaagaacag aagcaaaaac gcggcgcctt
      301 cctgatacgt catccgctgg tacgccgcca tccgtacagc gcacgcaaag cgctttatgc
      361 cgtgtccgga acgtcatttg gcatagaggg tatgccagac gcagacggca tcgcattgtt
      421 gcgggaattg gcagcgcatt caacgcagcc caagtaccag taccgcgttc agtacggtgt
      481 gggcgatgtg gtcgtatggg acaacgcttc tgtgctgcat tcggccacgc tgaccgatcc
      541 cttgtatccg cgcacgctgt tgcgcgtgac caccaaggaa gcgcagcgac catcggcctg
      601 acgcaggcag cgcatacggt tatggttacc gcggcaagat cagtcgtttt gttttttttg
      661 ttttcatgtg cggcactggc cgcgccgtat cccgacccca agcacggcga ggttgagctc
      721 cacgattttg atttcgccga cggcgaaatt ctcccatcgc tcaaacttca ctatttcacg
      781 ctcggtacgc cagcgcgtaa cgccgagggg cagatcatca acgcagtgct gctattgcat
      841 ggcacgggag gtcagggtac cggctttctg acgccggcct ttgcgggaga actgttcggc
      901 gttgggcagc cgctcgatgc caaccgctgg ttcatcatcg tgccagacaa cattggtcac
      961 ggccgctcga gcaagcccag tgatggcttg cgcgcgaaat tcccgcacta tggctaccgc
     1021 gatatggttg cagcacagcg gcgtctgctg tccgaaggcc tcggcgtaga acatctgcgc
     1081 ctgatcatcg gcacatcgat gggctgcatg catggctgga tgtgggctgg caactatccg
     1141 cagtccacgg atgcgcttct acccattgcc tgcctgcccg caccggtcgc gggccgcaac
     1201 cgcatggaac ggcgcttgat cgtggatgcg atccgcaacg atccggaatg gcagggcggc
     1261 gactatgcag cgcagcccca gggactcata accgcgctca ggatgatcgc catcagtgcc
     1321 ggcagcaccc ggcagcttta tcagcaggcg ccgactgtgg aggccaccga ccggttactg
     1381 gaccggatgg tgcaacagcg tttgcgcacc gcggacgcaa atgacctgct gtacgcctgg
     1441 gacgcgtcgc gcgattacga tccgggcgag aatctcgcgc gcatcagcgc gcacgtcacc
     1501 gcagtaaact tcgaggacga cgaacgcaat ccgccacagt tgcaagtcat ggaacggcag
     1561 gtagcccgcg tgccgcacgg aacatacgta ttgattccgg caagcgaccg cacgcgcggc
     1621 catgccagcc tcgccgacgc agtgctttgg aaagagtacc tggtgaacct gctcgcccgg
     1681 atttcccgct agcatcctaa agcggcttgc tcggatcgat cagcgtgcgc cacatatgcg
     1741 ctgttgcgct gctgtcgttg cccaaccctt ctttgggctg gcggaagtgg cggccgatga
     1801 tgtcggtgaa cggctcgatc gaccatggct gggatttgtc gaacgcataa tcgtcgcatt
     1861 tcgtgatgtt gcgcgaaatc atgtaccact tgtgcttgcg cgcatagtcg tgcgcttgcg
     1921 cgatatattc aggcggacga tagtcttcac cgaacatcgt gatatacatc tggggatatt
     1981 cgtagccgac cgatgcgtcg gggtaataac gcagcgcctg atggtaacgg atggcccagg
     2041 agactttttc cgatacgtaa ggttcgacca gctgcgcgcc ccaccagcca tgatcgggcc
     2101 gcacgacggc gagtccgata tcgtgcagca ggcaggcaag aatagtttct tcatccatgc
     2161 cggctttcat cgcctgcatg gcggtttgca cgcaatgcgg cgccgccgcc gtgggcaagc
     2221 cgaatttcgg cgtgttcagc cgcaggttga agatatcggc aagcgtgggc ttggccggca
     2281 taggtacgtc tggcgccggc gggcggatgc gcgcgaacgc ggcgccgatg tccatcaagt
     2341 cccgctgccg ttcaacactg agttcttcgc caggcatcat cgtttacctc atccaaagca
     2401 tgcccgcacc acgccgccat cgacgcgcag ggcggcgcca ttggtcgcag acgaaagtgg
     2461 actgcacacg taagccacca tagtggccac ttcctgcgtc gttgcatagc gcttcagcag
     2521 cgaactgggg cgcaccttct cgaaaaattc tttttcgaat tccgcaaacg gcctgccggc
     2581 tgcgcgtttc gcaatggccg ccttgttgcc atccgaaagc gtcgggcccg gcagtaccgc
     2641 attgaccgtg acgttggtgc ccacgcacgt ctctgccaac ccgcgtgaaa tcgcgagttg
     2701 cgcagtcttg gtcatgccgt aatgaatcat gttgtccgga atctggatac cgctctcgct
     2761 gctgatgaat acgatgcggc cccagttgcg ctgcttcatt cccggcaaat acgcacgcga
     2821 cagccgcacg ccgctcatga cgttgatgtc aaagaaacgc tgccattcgg cgtcatcgat
     2881 gtcctcgaac gctttgcgct cgaaaatgcc gaggttgttg accagaatgt ctacggtggg
     2941 gaagcgccgg atcagcgcat cagtcggtcc tgcggcagag agatcgccgg cgaaaccatc
     3001 cagcgcagcc cgaggcgtca cggcccggat gcgctccagc gctgcatcca ccgcagcctg
     3061 cgtgcgaccg ttcacaatga cttttgcgcc ctccgcagcg agcaacgctg cggtggcaaa
     3121 accgatgcct ttggtcgatc cggaaatcag ggccgattta tcctgcagct tcagatccat
     3181 tccggggttc cgtgagcgca caacccgttg cagaaatcgg cgaaggcaac gcgtggacta
     3241 ttggcgcgtt tactcggtgt cgcctggttc atccgaccaa cgccgcagcg gtccggcata
     3301 gtccgggtcc gcatttcctg catccgattc gtccgtttca tcatcgaatt tttccattgc
     3361 cttcagcagc gactgcaatc gcttttccgc gatcgtttcg cggtccggtt tgtcgccaat
     3421 tttctcgata accgatgcag cggcggaata atccttcttc gtcttgatgg gccgtgcaac
     3481 gccgttcaat ttatgggtgc gacctcttgt catgcttgcc cccttttggc aaaagagctc
     3541 cgtgcgcgag caattttccc ccctttggtc caggttcctg cccgttcatc gctccctgga
     3601 ttttgcgtac atgtcggcga caagctagtg taccgcacga tattgaccgg tgtttgccat
     3661 ccacgaagtc gcacgtctca tggaagcacc ctgaccagtg aacagcgtta gttgttgcgc
     3721 gggccgtcat acggatcgcg cttcgcggcc tcgtgttttg tgccgccccg tgatgccgcc
     3781 attgctctgt cattgtcgtc tgcagcccgg tatcgaaacc agccaacgtt catttcctcc
     3841 cacgtctggt cgccccagcg tacgacctgg ttcggatccg gattggcagg gttttgcggg
     3901 gagttgtccc aggtcaagtc cagcacgatt cgcgtgccgg cagggatgag cttgggcggg
     3961 tccaaggcat acaacggttg ccaactgaaa tcgtatttcg gcactgacaa caggatttga
     4021 tcgcttccat ccggatagtg cgcggtgaat ttcgccgacc
//
LOCUS       DATMZQ010000189         6256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3527933, whole genome shotgun sequence.
ACCESSION   DATMZQ010000189 DATMZQ010000000
VERSION     DATMZQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527933"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..631)
                     /locus_tag="VN496_06075"
     CDS             complement(113..631)
                     /locus_tag="VN496_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXF16550.1"
                     /translation="MKTEQKIVAVGAATGVITMAVSVWILTRLLPVPVIGDAPGERLA
                     YALLANVVALVPFFIMLITVGNSRFLSEAIDPTRRAESRSMEIDGRVADNTLQQNFVF
                     AVASLALSTVVAIQYLQVVWACAIIFVVARLVFWFGYRLNPLYRAPGMAATAYMNLGM
                     ILYLLFCIFFAR"
     gene            complement(736..1230)
                     /locus_tag="VN496_06080"
     CDS             complement(736..1230)
                     /locus_tag="VN496_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HXF16551.1"
                     /translation="MKPRSVQALGFVGWLLLCFAAAAAGAIASADAGAFYGRLARPSW
                     APPGWIFGPVWAVLYFSMALAAWLVWRDARNARAWQLALVLFLAQLAANALWSWLFFA
                     WHQGGSAFADIVLLWVLIVCTTVQFWRLKPLAGLLLIPYLAWVTFAAVLNWTVWQVNH
                     GALG"
     gene            complement(1303..1695)
                     /locus_tag="VN496_06085"
     CDS             complement(1303..1695)
                     /locus_tag="VN496_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXF16552.1"
                     /translation="MKADLVYLTYVSVLTALLWVPYILDRMAVRGLIDTVGYPENPKP
                     QSPWAQRLMKAHANAVENLVVFATLVLVANALGVTSDAVATAAVTYFWARLVHALAYA
                     FAMPWVRTLAFTVGFFAQAAVAWQILAR"
     gene            complement(1766..2161)
                     /locus_tag="VN496_06090"
     CDS             complement(1766..2161)
                     /locus_tag="VN496_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXF16553.1"
                     /translation="MFRGSCLCGSVRYEVRGCLGRATHCHCSMCRKAHGAAFATYGRV
                     QSTDFMFTSGADGIASYQSSPEVTRTFCKRCGSTLQFISTKRPGSFSLALGTLDDDPG
                     IRSFEHIFVGSKAPWFDIADGLPQHSTRP"
     gene            complement(2349..3251)
                     /locus_tag="VN496_06095"
     CDS             complement(2349..3251)
                     /locus_tag="VN496_06095"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXF16554.1"
                     /translation="MPSFIRIGLRSMLQSSHVVRPRRSLLFVPGLRPDRFAKALDSGA
                     DIVCVDMEDAVARSRKDEGRALTLPLFRDKSHPHVEKMVRINGLSTPDGLKDLTAIIE
                     LETPPPAIMIPKIRSAEEVQLIDSLLSSGPSRQIRFCVIVETNQGLERAMEIAKSSSR
                     IDSLILGAVDMSADLRCEKSWEPLLYTRSRLVHAAASAGIDLLDVPFLNLDDAEGLRQ
                     EAIGCARLGFTGKASIHPNQIATIHAAFTPDAKAIEKAQKVCAAFEQDTTGLVVVDGE
                     LIELPVVRSMYRVLAIAERTNRAA"
     gene            complement(3374..3958)
                     /locus_tag="VN496_06100"
     CDS             complement(3374..3958)
                     /locus_tag="VN496_06100"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HXF16555.1"
                     /translation="MSSSASVLPSSVVGSLWHAGKAVCNNAKDSLARRLICRAWSRSL
                     AIGSGVKPWPRADIWPVAQLEAPRHGAQLMVLAGASGRALAFGAGHVDGTPLPGESGN
                     AVISGHRDTHFAFLRDVRSGDVLAVHALPGRVARYVVSGIEVVRNKDVRVLLDVGDDR
                     LTLMTGYPFDSPVPGRRLRYVVVAKRIDAAVTRI"
     gene            complement(3955..4383)
                     /locus_tag="VN496_06105"
     CDS             complement(3955..4383)
                     /locus_tag="VN496_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16556.1"
                     /translation="MNTLSNRNLVSVPRVSAARDIVRIVIAAVLAGTGFAMLMALVVL
                     SLTVISPPAQASGAPALTSSGSADMTRATEDRSEAPAGTTFIAEAAAAQPLPTAVSAI
                     RSDEAPAASLPSLPKLAAWALVLALLVGAAVLVVRARSRQ"
     gene            4464..5285
                     /gene="pdsR"
                     /locus_tag="VN496_06110"
     CDS             4464..5285
                     /gene="pdsR"
                     /locus_tag="VN496_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HXF16557.1"
                     /translation="MESRGRNVAKKWHFTPVRLLHTAFGIVLQRAFTELKQGKQLHMA
                     RRIAIVEDDAAIRENYADALRRQGYEVTTFVDRPAAMKAMQTRLPDLALIDIGLANEI
                     EGGFALCRDLRALSSTLPIIFLTARDGDLDTVSGLRMGADDYLTKDISLPHLLARIAA
                     LFRRIEVMGSPAAAEDVLERGRLRLNVKRMEAHWATHQVELTLTEFWIVHALAKFPGH
                     VKDRDALMREASLTVDDSTITSHIKRIRRKFGALDSRFDSIQSVYGLGYRWHGES"
     gene            5272..>6256
                     /locus_tag="VN496_06115"
     CDS             5272..>6256
                     /locus_tag="VN496_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16558.1"
                     /translation="MASPELRGPGLRLKLFLAASILLLIPLLGFLYVRELENLLLKVQ
                     EQGVVATARAVGTALNDRPSLFLSGEIYPFALAQGNDLRIDNLPAPIVVDGQIEDWLQ
                     QPVAAHSVGAGAGGPETRAFSARYRIGRNGNAIYALFEVADAKVVLRDPERDTLQTTD
                     HLEIAVVTPDDEFLRFAIEARGTGKASVSLLAADGEAIPDPRIQAVFHLLPRGYLIEL
                     RLPRSLVGPRLGFTIVNADDRSGVTRVTSIGTSDTSSKQALGAVIVPSPEVSELVRRL
                     GRARSRIWVLDVNRRVIAQAGALRGPPAPADPGEGMFLRFWEKAADLLVRPI"
BASE COUNT         1356 a         1911 c         1843 g         1146 t
ORIGIN      
        1 aataaagctg gccgttacga tagcaaaagg agcaccagtt caggaatcag gtagccgaac
       61 cagcagcaaa cagcctggac gatcattggg ctcgtgttca aactcccggc cgtcatctgg
      121 cgaaaaaaat gcagaacaga agatagagaa tcatgcccag attcatgtaa gcagtagccg
      181 ccatccccgg cgcgcggtag agcggattga gccgatagcc aaaccagaac acaagtcgtg
      241 caacgacgaa gataattgcg cacgcccaca cgacctgcag atactgaatt gcaacgacgg
      301 tgctgagcgc gagcgacgcg acggcaaaca cgaaattctg ctgcagcgtg ttatcggcaa
      361 cgcgtccatc gatctccatc gatcggcttt ccgcccggcg cgtgggatcg attgcttcgc
      421 tgaggaaacg cgaattgccc actgtaatga gcatgatgaa aaacggcacc agcgcaacaa
      481 cattggcaag gagagcgtag gccaggcgtt cgccaggcgc atccccgatc acgggcacgg
      541 gcaggagcct tgtcagaatc cacacgctca cggccatggt tatcactcca gtggccgcac
      601 caacagcaac gattttctgt tccgttttca tgttttcagt cccatcgata caagacaggg
      661 cacttccttc gcggggagcc atgaactcac cgacgcggtg gaggccgtcg ccagaaataa
      721 atcgtctatc ggcgcttaac ccagggcccc atgattcact tgccatactg tccagttcag
      781 tactgccgca aatgtgaccc aggccaggta cgggatcaac agtaaccctg ccagcggttt
      841 gaggcgccag aactgaacag tagtgcagac gatgagtacc caaagcaaga cgatgtccgc
      901 gaaggcggaa cctccctggt gccaggcaaa aaacaaccag ctccagagcg cattggcggc
      961 gagctgagcg aggaacagga caagcgccag ttgccaggct cgcgcatttc tggcgtcccg
     1021 ccatacgagc caggctgcca gcgccatcga gaagtacaga acagcccaca cggggccgaa
     1081 aatccagccc ggtggagccc aggaaggacg agcaagtcga ccatagaacg cgccggcgtc
     1141 agcagacgcg atggctccgg ccgcagccgc agcaaagcac agaagcagcc agccgacgaa
     1201 tccgagcgcc tgtacggacc ggggcttcaa agataactcg tggttcattg aacacctgac
     1261 gggttcgatt cagcgctcca tcccattgag atgtccattt tcctatcgcg cgaggatttg
     1321 ccatgccact gccgcctgcg cgaaaaaccc caccgtaaag gccagagtgc gcacccacgg
     1381 catggcgaac gcgtaagcca gtgcatgtac caggcgagcc cagaaatagg tcacagcggc
     1441 tgtagcgacc gcatcactcg tcacaccgag cgcgttggcc acgagcacaa gcgtcgcgaa
     1501 caccaccagg ttttcaacag cgttcgcatg ggctttcatg agacgttggg cccatggcga
     1561 ttgcggtttt gggttctcag gatagccgac ggtatcgatc agcccacgta ccgccatgcg
     1621 atccaggatg tacggaaccc acaacagagc ggttagaacg ctgacataag tcaggtagac
     1681 aagatccgcc ttcatcattc ctccttcggt ttattgtagt tgcagcacgt ctccgtactg
     1741 attttttggc tcaggatccc ctggttcagg gacgagtact gtgttgcggc agcccatcgg
     1801 caatatcgaa ccacggagcc ttggagccga caaagatatg ttcaaagctt cgaatcccgg
     1861 gatcatcgtc gagagtcccc agcgctagcg aaaagctgcc gggccgcttt gtgctgatga
     1921 actgaagcgt cgagccgcag cgtttgcaaa atgtgcgggt gacctcagga gaagattgat
     1981 acgacgcaat accatccgcg cctgaagtaa acatgaagtc tgtcgattgg acgcggccgt
     2041 aggttgcaaa agcagctccg tgcgcctttc ggcacattga gcaatggcaa tgggtcgcgc
     2101 gaccaagaca accgcgtact tcgtagcgca cgctaccgca aaggcaactt ccccgaaaca
     2161 tcgctgctct cctcacaaat ctgcgtctgg ttcattgcaa ttcaattcat tcatcatagt
     2221 gcaatgcagg ttctgcctgg cgcaagagcc aaacacggga cagctcctgg cgatacaatg
     2281 aatccagcga gcaacaagct atttgccagg tcccgtaaag ccgtgacctg ttgtgtgcgg
     2341 agttgcgctc aggccgcccg gttcgtgcgc tcggcaatcg cgagcacacg gtacatcgag
     2401 cgcaccaccg gaagctcaat cagctcgcca tccacgacga ccagcccggt cgtgtcctgc
     2461 tcgaacgcgg cacaaacctt ttgcgccttc tcgatcgcct ttgcatctgg cgtaaacgcg
     2521 gcgtggatgg ttgcaatctg attcgggtgg atcgaggcct tgccggtaaa gccgaggcgc
     2581 gcgcagccga ttgcttcctg gcgcagtccc tcggcatcgt cgagattcag aaatggcacg
     2641 tccagcaaat cgatgccggc ggatgcggcg gcgtggacca gccgcgaacg cgtgtaaagc
     2701 aaaggctccc atgacttttc acagcgcagg tctgccgaca tgtcaaccgc gcccaggatc
     2761 agcgagtcaa ttcgtgacga agattttgca atctccatcg ctcgctccag gccctggttg
     2821 gtctcaacga tgacgcagaa gcggatctgg cgcgagggcc cggacgacag caaggaatcg
     2881 atcagctgta cttcttccgc ggagcggatc tttggaatca tgatcgccgg gggcggcgtt
     2941 tcaagctcga tgatcgctgt cagatccttc aagccatccg gcgtggacaa gccgttgatg
     3001 cgcaccattt tctcgacatg cgggtgcgac ttgtccctga ataacggcaa cgtcagcgcg
     3061 cgcccttcgt ccttgcggct gcgcgcgacc gcatcttcca tgtccacgca gacgatatcg
     3121 gcgccggaat ccagcgcctt ggcaaaacgg tcgggacgca gacccggcac gaacagcaag
     3181 ctcctgcgtg gccgcaccac atggctggat tgcagcatcg aacgtaatcc gattctgatg
     3241 aaagaaggca tgctagcctt acgccatcat ttcgcgcaac ccaatctgaa cgcaacagcc
     3301 agatgccaac atgaacggac tttgcatttc tccgtggctt cgtgacttct ggtgcgccgt
     3361 ttcgaatttt gttctagatc cgcgtgaccg ccgcgtcaat gcgtttggct acgacgacgt
     3421 agcgcaaccg acggccgggc acaggactgt cgaagggata acccgtcatc aacgtcaggc
     3481 gatcgtcgcc aacatcgagc agcacgcgca catccttgtt gcgcacgact tcgatgccgg
     3541 atactacata acgcgccacc cggccgggca acgcgtgcac tgccagaaca tcgcctgaac
     3601 gcacgtcgcg aagaaatgcg aaatgcgtgt cgcggtgtcc gctgatcacg gcattgccgg
     3661 actcgcctgg cagcggcgtg ccatcgacgt gaccggcgcc gaaggccagc gcgcgtccgc
     3721 tggcaccggc cagtaccatg agttgcgcgc cgtgccgcgg cgcctcgagc tgggcgaccg
     3781 gccagatatc ggccctcggc caaggcttta cgccggagcc gatcgcgagc gaccggctcc
     3841 acgcgcggca gatcagccgt cgcgccaaag aatccttcgc gttgttgcat acggccttac
     3901 cggcatgcca tagcgatcca acgaccgagg aaggcaagac cgatgcggac gaactcactg
     3961 cctgctccgc gcacgaacga caagaaccgc ggcgcccacc agcaatgcaa gcaccagcgc
     4021 ccacgccgcc aacttcggca agctgggcaa tgacgctgcc ggcgcttcgt cactacggat
     4081 cgccgatacg gcagtgggca ggggttgcgc tgcagcggct tccgcgatga acgtggtgcc
     4141 cgcaggtgcc tcagatcgat cctcggttgc gcgggtcatg tcggcagacc cggacgatgt
     4201 cagtgccggc gcgccagaag cctgcgcagg gggcgagatt acggtcagcg aaagcaccac
     4261 gagcgccata agcatggcaa atccggttcc cgcgagcact gcagcgatga cgatgcgaac
     4321 gatatcgcgg gccgctgaaa cgcgcggcac gctgactaga ttgcgattcg atagagtatt
     4381 catattggcc tcaggcatga gaatgtgcgt tattgcacgc gagtcattgg aagcctgcat
     4441 cggggcggca gcgggccagc gccatggaaa gtcgcgggcg gaatgtggca aaaaaatggc
     4501 atttcacgcc cgtgcgcctg ctgcacactg ctttcggtat agtgctgcaa cgggctttta
     4561 cagagctaaa acaagggaaa cagttgcata tggcaaggcg catcgctatt gtcgaagacg
     4621 acgccgcgat ccgcgaaaac tacgcggatg cgttgcgacg gcaaggttac gaagtcacca
     4681 cctttgtcga tcgaccggct gccatgaagg ctatgcagac tcgtcttccc gatctcgcgc
     4741 tgatcgatat cgggctcgcg aacgaaatcg aaggcggctt cgcgctgtgc cgggatttgc
     4801 gcgctctgtc ttccaccttg ccgatcattt ttctgactgc gcgcgacggc gacctggaca
     4861 ccgtgtcggg actgcgcatg ggggcggacg attacctgac aaaggacatc agcctgccgc
     4921 atctgctggc ccgcattgca gcgctgttcc gccgcattga agtcatgggc tcgccggcgg
     4981 ccgccgagga cgtgcttgaa cgcggccgcc tgcggctcaa cgtcaaacgc atggaagcgc
     5041 actgggccac gcaccaggtc gaactcacct tgaccgaatt ctggatcgtg catgcgctcg
     5101 ccaaatttcc tggccatgtg aaggaccgcg acgcgctgat gcgggaagcc agcctgacgg
     5161 tggacgacag taccatcaca tctcatatca agcgcatccg ccgcaagttc ggcgcgctcg
     5221 attccaggtt cgattcgata cagagcgtgt acggcctggg ctatcgctgg catggcgagt
     5281 cctgagttgc gcggaccggg attgcgcctg aagctgtttc ttgcggccag cattctgctg
     5341 ctgattccgc tgctcggatt tctgtatgtg cgcgaactcg agaacctgct gctcaaagtg
     5401 caggaacagg gcgtggtcgc taccgcacgc gctgttggaa ccgcgctgaa cgaccggccc
     5461 agcctgttcc tgagtggcga gatctatcct tttgcattgg cgcagggcaa cgatttgcgc
     5521 atcgacaatc tgcccgcccc cattgtcgtg gacggccaga ttgaggactg gctgcagcag
     5581 ccggtcgccg cgcattccgt cggcgccggc gcgggggggc cggaaacgcg cgctttctcg
     5641 gctcgctacc gcatcggcag aaacggcaat gccatctatg ccctgttcga agtcgcggat
     5701 gcaaaggtag tcttgcgcga tcccgagcgc gatacgctgc aaacgaccga ccacctagaa
     5761 atcgccgtgg tcacgccgga cgacgagttc ctgcggtttg ccatcgaggc ccgcggcaca
     5821 gggaaagcgt cagtatccct gctggccgcg gatggcgagg caattcccga tccgcgcatt
     5881 caggccgtgt tccacttgct gccacgcggc tatttgatcg agctgcgctt accgcgctcg
     5941 ctagtcggtc cgcgtcttgg attcacaatc gtcaatgcag acgatcgctc aggcgttacc
     6001 cgcgtcacgt cgatcggcac ttccgatacg tcgagcaaac aggcgctggg cgcggttatc
     6061 gtgccctctc cggaagtcag cgagctggtg cggcggcttg gtcgggcacg gtcgcgcatc
     6121 tgggtgctcg acgtcaatcg gcgtgtaatc gcacaggccg gcgccctgcg cgggccgccc
     6181 gcgccagccg accctggaga aggcatgttt ttgcgtttct gggaaaaggc agccgacttg
     6241 ctggtgcgac cgatcc
//
LOCUS       DATMZQ010000190        10860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1874933, whole genome shotgun sequence.
ACCESSION   DATMZQ010000190 DATMZQ010000000
VERSION     DATMZQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874933"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VN496_06120"
     CDS             complement(<1..218)
                     /locus_tag="VN496_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXF16559.1"
                     /translation="MKTNLAQFIRDTPAGKEADSILRTCVHCGFCTATCPTYQLLGDE
                     LDSPRGRIYLMKEMLEGNEVTAKTQIHL"
     gene            complement(245..1297)
                     /gene="glcE"
                     /locus_tag="VN496_06125"
     CDS             complement(245..1297)
                     /gene="glcE"
                     /locus_tag="VN496_06125"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HXF16560.1"
                     /translation="MRDVLEKFSETIRQAAAEHTQLRIRGSGSKDFYGLALIGDVLDT
                     CTLTGIVDYEPTELVMTARAGTRLRDVEKTLADAGQMLAFEPPHFGPEATLGGCIASG
                     FSGPRRAAAGSARDFVLGVRMINGIGEALHFGGQVMKNVAGYDLSRLMTGSFGTLGLI
                     TEISLKVLPQPEQELTLRFELDETQAIASMNAWAAKPLPVSATCHHERALHVRLAGAA
                     VAVGAAHKKLGGETVPDGAQFWQSIREQSHPTFAHAPALWRFSIKPTSPPLGLGKQVM
                     EWNGSLRWVAEDLDPSRAFAIAHRADGHATLFRSGNKRHGIQQLSAGLLAVHKKLKRA
                     FDPHGILGFGRIHADF"
     gene            complement(1372..2871)
                     /locus_tag="VN496_06130"
     CDS             complement(1372..2871)
                     /locus_tag="VN496_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXF16561.1"
                     /translation="MGMPALLSSEILQPQIAHAELVAQLTSVLPPASLLHEREDLKPF
                     ECDGMTAYQRLPLMVALPETEDQVREVLRICYRGRVPVVFRGAGTGLSGGALPYAYGL
                     LLVMAKMKHIVHIDALARSARVQPGVRNAAISEATSPYGLYYAPDPSSQIACSIGGNV
                     AENSGGVHCLKYGLTVHNILKLRFVTIEGELLEIGSDGLDAPGYDLLALITGSEGMLG
                     VVTEITVKLLPKPEKAQVVLAAFDDIAKAAQAVADVIGAGIIPAGLEMMDKKATAAVE
                     QFVHAGYPLDAAAILLCESDGTAEEVSDEIERIRQVMNTAGATEVRTSRDESERLKFW
                     AGRKAAFPAVGRILPDYYCMDGTIPKKHLPYVLKRIDELSEQFHLQCANVFHAGDGNL
                     HPLIMFDANEGNQNERAEAYGAAILELCVEVGGTITGEHGVGLEKINQMCVQFNTGEL
                     KAFHAVKAVFDEHGLLNPGKAVPTLQRCAEFGAMHVHRGQENFPELPRF"
     gene            complement(2888..4150)
                     /locus_tag="VN496_06135"
     CDS             complement(2888..4150)
                     /locus_tag="VN496_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HXF16562.1"
                     /translation="MNQDPRVLLRTMLDAAIAAALPDKTLPAYLPSAPRGRMLVIGCG
                     KAAGSMAKAVEDHWPGELSGMVVTRYGYKVPTSRIEVIEAAHPVPDEAGRRAAERMLA
                     MVRGLSPDDLVLFLVSGGGSSLLSLPAPGLTLGDKQAINKALLKSGATISEMNCVRKH
                     LSAVKGGRLAAACAPARVITLAISDIPGDDPAVIASGPTVADATTFADARAILDKYHI
                     IEPGKVIEHLLAAKEETPKPGDPRLAKTELHMIAAPQMSLEAAAEAARRAGVTPVILG
                     DAIEGEAREVAIVHAGIARQVKRHGQPANRPCVLLSGGETTVTVRGKGRGGRNAEFLL
                     ALAVALGAEPDVYALAGDTDGIDGTEDNAGALLTPDTLMRAAAHRLDAKRMLADNDGY
                     SFFSGLGDLVMTGPTLTNVNDFRAILII"
     gene            complement(4184..5065)
                     /gene="glxR"
                     /locus_tag="VN496_06140"
     CDS             complement(4184..5065)
                     /gene="glxR"
                     /locus_tag="VN496_06140"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HXF16563.1"
                     /translation="MNVGFIGLGIMGKPMVANLIKGGHALFLASRSGVPQELISAGAK
                     ACASAREVAQKADVIITMVPDTPDVEKVLFGKEGVAEGLSRGKIVVDMSSISPIETKA
                     FAERVRKTGADYVDAPVSGGEVGAKNAALTIMVGASEESFARVKPLFELMGKNITLVG
                     ETGAGQTCKVCNQIIVALNIEAVGEALLLASKAGVDPGKVRQALMGGFAASRVLEVHG
                     ERMVKRTFDPGFRIELHQKDLNLALSSARALKMSLPNTATAQELFSACQAQGGGKWDH
                     SAMVKALETLANHQVAK"
     gene            complement(5135..5914)
                     /gene="otnI"
                     /locus_tag="VN496_06145"
     CDS             complement(5135..5914)
                     /gene="otnI"
                     /locus_tag="VN496_06145"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HXF16564.1"
                     /translation="MPNFNANLSMMFNEVGFLERFGAAAKAGFRGVEFLFPYDFPAQQ
                     IRDQLDKHKLEMVLFNMPPGDWAAGDRGLACDPARITEFQDNIGKAVEYANELDCAMV
                     HCMAGLKPRGVSEDKMRETYIANLQFAGKEFAKYHMKLLIEAINTRDIPGFYLNYSRQ
                     AFDIMHYANVPNLLFQYDIYHMQIMEGDLAPTVEKNLTKIGHMQLAGTPGRHEPNTGE
                     INYPFLFGFFDRIGYKGWIGCEYRPAAKTTDGLGWLRPYIK"
     gene            6019..6864
                     /locus_tag="VN496_06150"
     CDS             6019..6864
                     /locus_tag="VN496_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXF16565.1"
                     /translation="MKFRGTDSYIATDDLMMAVNAAVTLERPLLIKGEPGTGKTMLAM
                     EVARALDRPLLQWHVKSTSKAQQGLYEYDAVSRLRDSQLGDGRVNDIANYIVRGPLWD
                     AFDSERQTVLLIDEVDKADIEFPNDLLRELDRMEFYVYETQQLVKARHRPVIIITSNN
                     EKELPDAFLRRCFFHYIRFPDKQTMQRIVDVHFPNLKKSLLREALEVFFEIRDVPGLK
                     KKPSTSELLDWLKLLMAEDIPPETLRGKDQSKIIPPLAGALLKNEQDVHLFERLVFMT
                     RRAGG"
     gene            7067..10036
                     /locus_tag="VN496_06155"
     CDS             7067..10036
                     /locus_tag="VN496_06155"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXF16566.1"
                     /translation="MIRLSATVILLWIFTVSGFSGLIYESIWSHYLKLFLGHAAYSQV
                     LVLVIFMGGMAIGSALAARFSTRWKRVLLAYALVEGITGAIALVFHGAFDSVTQASFA
                     HVIPQLHSATAVHAFKWSLGALMILPQSILLGMTFPLMSAGFVRLAPDAAGRSVAMLY
                     FTNSLGGAVGVLVSGFVLIPNVGLPGAIMTAGLLNILVGLATWLLVKKIPETRQVPAP
                     DALRPAVAGKKVVDYRVLLFASLITGMASFCYEIGWLRMLSPVLGASTHAFELMLSSF
                     ILGLALGGLWIRRRIERSADPVALLAGIQLAMGLLALLTIVLYGSTFEWMGAVMNTFA
                     RTQQGYVGFNWASHAISLAVMLPATFCAGMTLPLITFAALRGGGGERAIGSVYAANTV
                     GAIIGVAISVHMLMPLFNAKGVIVAGAALDFGLGLLLLRRIAGNFRISPLAVASFLGS
                     IVFLWVVLAVDVDPLKVVSSVFRHGISRSVDDEVLYMRDGKTATVTLSRNTSVIALAT
                     NGKVDASVNMGDGPATQDEETQSLLAALPLTLQDSIKQVAVIGFGSGMSSNVLLADRR
                     VDRVDTIEIEARMIEAARQGFFPRNRLSYEDPRSKLYVEDAKTFFSLTGRKYDLILSE
                     PSNPWVSGVSSLFTHEFYRHVAGYLNEGGLLAQWMQLYEIDANSLASVVKALSPLFSD
                     YVIYMADDTNILIVARRGGLVGEPGAALLSQPEIAEQLARVGVDSIDDIRARWIGNKA
                     LLDAYFNRSAAPMNSDYFPFMDHEAPKARFMRSNAFELVRLNAAGLPIPEMLGGQDRQ
                     RQPWRQLTPRNPADRKLAQLVIRSIMDGTDSPELGPQRQDVLLTLMGAHDCGGTLGRK
                     WETAWTNVGRYAIRYLDRETTPAFLNKLLPAQCRAKLSVNGKIWLPLLQAVAARDADA
                     MITHAGRLLELPNATALPGDTLYAAGISMLGMLSREEPERALAVKKRLTRELLPNVPV
                     PFEILWMEALAQRSQMR"
     gene            10053..>10860
                     /locus_tag="VN496_06160"
     CDS             10053..>10860
                     /locus_tag="VN496_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXF16567.1"
                     /translation="MLTDFFLRLRTGGIPVSVKEYLTLMEALQNKLSAGSLDEFYYLA
                     RACLVKDESNYDKFDRVFGAYFKGLVTLPDNGIVDLPEEWLRKQAELLLTEEEKKLVE
                     SLGGWEKLMETLRKRLEEQRGRHQGGSKWIGTGGTSPFGAYGYNPEGVRIGQDGSRNR
                     SAVKVWDRREYRNLDDSLELGVRNIKVALKRLRKFAREGAAEELDLDSTIRSTAKNAG
                     YLDIKMVPERHNAVKVLLFLDVGGSMEGYVKVCEELFSAARVEFKHLEYFY"
BASE COUNT         2099 a         3174 c         3228 g         2359 t
ORIGIN      
        1 tcaaggtgga tctgcgtttt cgcggtcact tcgttgcctt cgagcatttc cttcatgagg
       61 taaatgcgcc cgcgcgggct gtccagttca tcgccgagca attgataagt cgggcaggtc
      121 gccgtgcaga agccgcagtg gacacaggtg cgcaggatgc tgtccgcttc cttgcctgcc
      181 ggcgtgtccc ggatgaattg cgcgagattc gttttcattt caattgatcg atattcgcga
      241 cagctcagaa atcggcatgg atgcggccaa acccgaggat gccgtgcgga tcgaacgcgc
      301 gcttgagttt cttgtgcaca gcgagcaggc cggctgacaa ctgctggatg ccatggcgtt
      361 tgttgccgct gcggaacagc gtggcgtggc cgtcggcccg gtgcgcgatc gcaaacgccc
      421 gcgacggatc gagatcttcg gcgacccagc gcaggctgcc gttccactcc atgacttgtt
      481 tcccaagacc caacggtggc gaagtcggct tgatcgagaa acgccagagc gcgggcgcat
      541 gcgcaaacgt tggatgggac tgctcgcgga tcgactgcca gaactgcgca ccatccggga
      601 ccgtttctcc gcccagcttc ttgtgcgcgg cgccaactgc cactgccgcg ccggccagac
      661 gcacgtgcaa agctcgctcg tggtggcaag tggccgagac cgggagcggt ttggccgccc
      721 aggcgttcat ggatgcaatg gcctgcgttt catcgagctc gaagcgcaaa gtcaattcct
      781 gttcgggctg cggcaatacc ttcagggata tttcagtgat caagcccagc gtaccgaacg
      841 acccggtcat caggcgcgac aaatcgtagc ccgctacatt tttcatgacc tgtccgccaa
      901 agtgcaacgc ttcaccaatc ccgtttatca tgcgtacgcc gagcacaaaa tcgcgcgcgg
      961 aaccggccgc agcccggcgt ggtccggaga agccagaggc aatgcaaccg ccgagcgtag
     1021 cttcggggcc gaagtgaggc ggttcgaatg ccagcatttg cccggcatcc gcgagtgttt
     1081 tttcaacgtc gcgcaaacgc gtgccggccc gcgcggtcat gaccaactcg gtaggttcgt
     1141 aatcgacgat gccagtcagc gtgcacgtat cgagcacatc gcctatcagg gcgagaccgt
     1201 agaagtcctt gctgccgctg ccgcgtatgc gcagctgggt gtgctcggct gctgcctggc
     1261 gaatggtttc tgaaaatttc tcgagtacgt cccgcatgaa atcgtttctt tgcgccttgc
     1321 gttgagaccc ttcccggctg aggcagttgc gtcagcgcga gcggctggac atcagaaacg
     1381 cggcagttcc ggaaagttct cctgtccacg atgcacgtgc atggcaccga attcggcaca
     1441 gcgttgcaag gtcggaacgg ctttgccggg attgagcagg ccatgctcgt caaacacagc
     1501 cttgacggca tgaaaagcct ttagttcgcc agtgttgaac tgcacgcaca tctggttgat
     1561 cttttcgagc ccgacgccat gctcgccggt gatcgtgccg cccacttcga cacagagctc
     1621 cagaatcgcc gcaccgtagg cttccgcgcg ctcgttctgg ttgccttcgt tggcatcgaa
     1681 catgatgagc ggatgcaagt tgccgtcgcc cgcatggaat acattggcgc attgcagatg
     1741 aaactgttcc gacagctcat ctatgcgctt cagcacgtaa ggcagatgtt tctttggaat
     1801 ggtgccatcc atgcagtaat aatccggcag gatgcgtcca acggcgggga aggcagcctt
     1861 gcggccggcc cagaatttca gccgctctga ctcatcgcga gacgtacgga cctcggttgc
     1921 gccggccgtg ttcatgactt gcctgatgcg ctcgatctca tcgctgactt cttctgcggt
     1981 gccgtccgat tcacacagca agattgccgc ggcgtccagc gggtagccgg cgtgcacgaa
     2041 ctgctcaacc gccgcggttg cttttttatc catcatttcc agtccggcgg gaatgatgcc
     2101 cgctccgatc acgtccgcta ctgcttgcgc cgccttggca atgtcatcga atgcagcgag
     2161 cacgacctgc gctttctcag gcttgggcaa taacttgaca gtgatttcgg taacgacgcc
     2221 cagcatgcct tcggaacccg tgatcagcgc cagcaagtca tagccgggcg catcgagtcc
     2281 atccgaaccg atttccagca actcaccttc aatggtcaca aagcgcaact tgaggatgtt
     2341 gtgcaccgtc agtccgtact tgaggcaatg cacgccacca gagttttccg ccacgttgcc
     2401 gccgatggaa caggcgatct ggctggacgg gtcgggcgca tagtacagac cgtacggcga
     2461 cgtcgcctcg gatatcgccg cgttacgcac gccgggttgc acgcgagccg agcgcgcaag
     2521 cgcatcgatg tgcacgatgt gcttcatttt tgccatgacc agcagcaggc cgtacgcata
     2581 cggcagcgcg ccgccggaca gcccggtccc ggcaccgcgg aataccaccg gcacgcggcc
     2641 gcggtaacaa atgcgcagaa cttcacgaac ctggtcttct gtttccggca acgccaccat
     2701 tagcggcagg cgctgatagg cagtcatgcc gtcgcattcg aacggcttga ggtcctcgcg
     2761 ctcgtgcagc agcgatgcgg gcggcaatac cgacgtcagt tgcgcaacca gttctgcgtg
     2821 cgcaatctgc ggctgcagta tttcggacga cagcaatgcg ggcatgccca tgatcggatg
     2881 agttcggtca gataatcagg atcgcgcgga aatcgttgac attagtcagc gtcgggccgg
     2941 tcatcacgag gtcgccgaga cctgagaaaa aactgtaccc gtcattatcg gcgagcatcc
     3001 tttttgcgtc cagtcgatgc gccgccgcgc gcatcagagt atccggcgtg agcaaggcgc
     3061 cggcgttgtc ttccgttcca tcgatgccat cggtgtcgcc agcgagcgcg taaacgtcgg
     3121 gttccgcgcc aagcgcgacg gcaagcgcca gcaggaactc ggcgttgcgc ccgccgcggc
     3181 ccttgccgcg cactgtaacc gtcgtttctc ctcccgacag tagaacacag ggcctgttcg
     3241 ccggttgccc gtgacgcttg acctggcgcg caatgcccgc atgcacgatg gcgacttcgc
     3301 gcgcttctcc ttcaatcgca tcgccaagga tgaccggggt gacgcctgcc cgacgcgcag
     3361 cttccgcggc ggcttccagg gacatctgcg gtgccgcgat catgtgcaac tcggttttcg
     3421 ccagccgcgg atcgccgggc ttcggggttt cttctttggc agcgagcaga tgctcgatta
     3481 cttttcctgg ctcgatgatg tggtatttat cgaggatggc gcgcgcgtcc gcaaaagtag
     3541 ttgcgtcggc aaccgtgggg cctgaagcta tcactgccgg atcatcgccc ggaatatcgg
     3601 aaatggcgag tgtgatgacc cgggcgggcg cgcatgcggc cgccaggcgg ccgcccttga
     3661 cggcggaaag atgtttgcgc acgcaattca tctcgctgat agtggcgccg cttttcagca
     3721 atgccttgtt gattgcctgt ttgtcgccca gcgtgagacc gggcgccggc aatgacagca
     3781 gcgatgatcc cccgccggat acgaggaaca acaccagatc gtccggcgac aggcctcgca
     3841 ccatggcgag catgcgttcg gccgctctgc gcccggcttc atccggcacc gggtgagcgg
     3901 cttcgatgac ttcaattctg ctggtgggca ctttatagcc gtagcgggtc actaccattc
     3961 ccgacaattc gcctggccag tgatcctcga ccgcttttgc catgcttccc gctgccttgc
     4021 cacagccgat caccagcata cggcctcgcg gagcggatgg caggtaggcg gggagcgtct
     4081 tatccggcag ggcagcggcg atcgctgcat ccaacatggt tctcagcaga acgcgtggat
     4141 cctgattcat gcttgcaccg gactcaagcc aggaatttgc ccgttacttg gctacctggt
     4201 gattggccag tgtttccagc gccttcacca tggccgagtg gtcccacttg ccaccccctt
     4261 gggcctggca cgcgctgaat aattcctgcg ccgtggcagt attcggcagg ctcatcttca
     4321 gcgcacgcgc cgacgaaagc gcaaggttca gatccttctg atgcagttcg atgcgaaagc
     4381 ccggatcgaa agtccgtttc accatgcgct ccccatggac ttcaaggact cgcgacgccg
     4441 cgaaccctcc catcaatgcc tggcgcacct tgccggggtc gacaccggcc tttgacgcaa
     4501 gcagcagtgc ttcaccgacc gcttcgatat tcagcgccac gatgatctgg ttgcagacct
     4561 tgcaggtctg gcccgcgcct gtttcgccaa ccagcgtgat gttcttgccc atcagttcga
     4621 atagcggttt cactctggcg aaactctctt ccgaggcgcc gaccatgatc gtcaatgccg
     4681 cattcttggc gccgacttcg ccgcccgaca ccggagcatc gacataatcg gcgccggttt
     4741 ttcggacgcg ttcggcgaat gccttggtct cgatcgggga aatggaactc atgtcgacca
     4801 cgatttttcc gcgggacaag ccctcggcca cgccctcttt gccgaacagt actttctcga
     4861 catcgggggt gtcaggcacc atcgtgatga tgacatcggc cttttgtgcg acttccctgg
     4921 cggaggcgca cgccttcgcg cccgcagaaa tgagctcctg tggtacgcca ctgcgactcg
     4981 ccaggaacag cgcatggccg cctttgatga ggttggcgac catgggcttg cccatgatgc
     5041 ccagtccgat gaagccgacg ttcatgctgt tgtctccttg aaaaccaaac tcatgccacg
     5101 acctgctgcg gggaacgcag gattgccggg tggtttattt tatataaggc ctcagccagc
     5161 cgaggccgtc ggtggttttg gccgccgggc gatactcgca gccaatccag cctttgtagc
     5221 cgatgcggtc gaaaaaaccg aacaagaacg gatagttgat ttctccggta ttcggttcgt
     5281 ggcgcccggg cgtgccggcc agctgcatat gaccaatctt ggtcaggttt ttctccaccg
     5341 ttggtgcgag atcgccttcc atgatttgca tgtgatagat gtcgtactgg aacagcagat
     5401 ttggcacgtt cgcgtagtgc atgatatcga atgcctggcg cgaataattc agatagaacc
     5461 cggggatgtc gcgcgtattg atggcttcga tcagcagctt catgtgatat ttcgcgaatt
     5521 cctttccggc aaattgcaga ttggcaatgt acgtctcgcg catcttgtct tccgacacac
     5581 cgcgcggctt caggccggcc atgcaatgga ccattgcaca atccagttca ttggcatact
     5641 cgactgcctt gccgatgttg tcctggaatt ccgtgatcct tgccggatcg caggccaggc
     5701 cccgatcgcc cgccgcccag tccccgggag gcatgttgaa gaggaccatc tccagtttgt
     5761 gcttgtccag ctgatcgcgt atctgctgtg cggggaagtc atacggaaac agaaattcga
     5821 cgccgcgaaa accggctttc gcagcggcgc cgaaacgctc caggaagccc acctcattga
     5881 acatcatgga aaggttggcg ttgaaattag gcatgcatcc tcccggggat ttattagtcg
     5941 taaggtttgc gggaccggca attatagccg tccacgccgt tataatcggc gcctgatccg
     6001 gatgcgaggc aggtgttcat gaaattcagg ggaaccgact cttatattgc taccgacgac
     6061 ttgatgatgg ccgtcaacgc ggccgtcacg ctggaacggc cgctgctgat caagggcgag
     6121 ccgggcaccg gcaagaccat gctggcgatg gaggttgcca gagcgctcga tcgtccacta
     6181 ctgcaatggc atgtgaaatc gacttccaag gcacagcagg ggctgtacga atatgatgcg
     6241 gtttcacgtt tgcgcgattc acagcttggc gacggccgtg taaacgatat cgccaattac
     6301 atcgtgcggg gtccgctgtg ggatgctttc gactctgagc gccagaccgt gctgctgatc
     6361 gatgaagtgg acaaggcgga cattgaattt ccgaacgacc tgttgcgcga actcgaccgg
     6421 atggagttct atgtctacga aacgcagcaa ttggtaaagg cgcggcaccg gccggtcatc
     6481 atcatcacca gcaacaatga gaaggagctg ccggacgctt tcctgcgccg ctgcttcttc
     6541 cactacatcc ggtttcccga caagcaaaca atgcagcgca tcgtcgacgt gcattttccg
     6601 aatctcaaaa aatcgctgct gcgcgaagca ctggaagtat tttttgaaat acgcgatgtg
     6661 ccgggcctga agaaaaagcc gtccacgtca gaactgctcg actggttgaa gttgctcatg
     6721 gccgaggaca ttcctccgga aaccctgcgc ggcaaggatc aatccaagat aattccgccg
     6781 ctcgccggcg cgcttctaaa gaatgagcag gatgtccacc tgttcgaacg tcttgtattc
     6841 atgactcgca gagcgggcgg ctagcaggct tgctgaatgc ctttttgcca ggagctaacc
     6901 gcggtcacaa gcgcggtgga tcgtctgcac gcatgcattc tcctatccga tttcaaaacg
     6961 gcaacgctcg gtctgaagtc atggtgactt ccgggttggc gcggcggtgt gtcggggttc
     7021 ggattagggc aaagtttctt tccaaaaaaa cgcagggggt tgttcgatga tcaggttatc
     7081 cgccactgtg attctgctgt ggattttcac cgtttcaggt ttttccggcc tgatctacga
     7141 gtcgatctgg agccactacc tcaaactctt tctcgggcac gctgcctatt cccaggtgct
     7201 ggtactcgtg attttcatgg gtggaatggc gatcggttcc gcccttgccg cacgtttctc
     7261 gacgcgctgg aaacgcgtgt tactggccta tgctctggtg gagggcatca ccggcgccat
     7321 cgcgctggta ttccatggcg cattcgacag cgtcacgcaa gcctcttttg cgcacgttat
     7381 tccgcaattg cattcagcta ctgcggtgca cgcgttcaaa tggagtctcg gcgcactgat
     7441 gatcctgcct caatccattc ttctgggcat gaccttcccg ctcatgagtg ccggtttcgt
     7501 gcgtcttgcg ccggatgcgg cggggcgcag cgtcgccatg ctgtatttta cgaacagcct
     7561 tggcggcgcc gtcggcgtcc tggtgagcgg ctttgtgctc attccaaatg ttggcttgcc
     7621 cggcgccatc atgacagccg gactgttgaa catcctggtt ggcttggcga cttggttgct
     7681 ggttaaaaag attcccgaga cccgacaagt cccagccccg gatgccttgc ggcccgctgt
     7741 tgctggaaag aaagttgtgg attaccgggt cctgctgttc gcttccctca taaccggcat
     7801 ggcatcgttc tgctacgaga taggctggct cagaatgctg agtccggtgc tgggcgcaag
     7861 cacgcatgca ttcgaactga tgttgtcatc ttttatcctg ggcctggcct tgggcgggct
     7921 gtggatcagg cgacgcatcg agcggagcgc tgatccggtt gcgttgctgg ccggcattca
     7981 gttggcaatg ggtctgcttg cgctactcac gatcgtccta tacggcagca cgttcgaatg
     8041 gatgggggcg gtcatgaaca cattcgcgcg cacgcagcaa ggctacgttg gcttcaattg
     8101 ggcaagccat gccatatcac tggcggtgat gttgccggcg actttctgcg ccggcatgac
     8161 gctgccgctg attacttttg cggcattgcg cggtggcggc ggtgagcgcg cgatcggttc
     8221 ggtgtacgcg gccaacacgg tcggcgcgat tattggcgtt gccatatccg tgcatatgct
     8281 gatgcccctg ttcaacgcaa aaggggtcat tgtggcgggt gctgcgctcg atttcggtct
     8341 ggggctgctg ctgttgcgca gaatcgccgg caatttccga atctccccgc tcgccgtggc
     8401 gtcctttctg ggttcgattg tatttctctg ggtcgtgctg gcggtcgacg ttgatcccct
     8461 gaaggtcgta tccagcgtgt ttcgccacgg catcagccgt tcggtggacg atgaagtgct
     8521 ctacatgcgc gatggaaaaa cggcgaccgt cactctgtca cgcaacacga gcgtcattgc
     8581 ccttgccacc aacggcaagg tcgatgccag cgtcaacatg ggagacggtc ccgctacaca
     8641 agatgaggaa acgcagtcac tgttggcggc cttgccgctg acgctgcaag attcaatcaa
     8701 gcaggttgca gtaatcggct ttggttccgg catgagttcg aacgtgctgc ttgcagacag
     8761 gcgcgttgac cgcgtcgata ccatcgaaat cgaggcgcgc atgatcgagg cggcccgcca
     8821 gggatttttc ccgcgcaacc ggctttccta cgaggatccg cgcagcaagt tgtacgtcga
     8881 ggatgccaag acgttttttt cactgactgg ccgcaagtat gaccttattc tttcggaacc
     8941 ttccaatccc tgggtcagcg gcgtttcttc gctgttcacg cacgagtttt accgccatgt
     9001 ggccggctac ctgaacgaag gcggcttgct tgcgcaatgg atgcagctct atgagatcga
     9061 tgcaaattca ctagcttccg tggtcaaggc gttgtcgcca ttgttttccg attatgtgat
     9121 ctacatggca gacgatacca atatcctcat cgtggcgcgg cggggtggac tggttggaga
     9181 gccgggagca gcactgttgt cgcagccgga aattgccgaa caactggcgc gcgtgggcgt
     9241 ggattccatc gacgacatac gcgcccggtg gatcggcaac aaagccctgc tagatgccta
     9301 tttcaaccgc tcggctgcgc cgatgaattc ggattacttc ccgttcatgg atcatgaagc
     9361 gccgaaagcg cgcttcatga gatcgaatgc attcgagctg gtacgcttga atgccgccgg
     9421 actgccgata cccgaaatgc ttggcggaca ggaccgccag cgccaaccgt ggcgacagtt
     9481 gacgccgcgc aatcctgctg atcgcaagct tgcccagctg gttattcgca gcatcatgga
     9541 cggaaccgat tcacccgagc tcggacccca gcgccaggac gtgctattga ccctgatggg
     9601 cgcgcacgat tgcggtggca cgcttggccg taagtgggaa acggcgtgga ctaatgtcgg
     9661 ccgttatgca attcgttacc tggaccgcga aactactccc gcctttctaa acaaactgct
     9721 gcccgcgcag tgccgtgcga agctttcggt gaatggcaaa atatggctgc cgttactgca
     9781 agccgtcgct gcgcgcgatg cagacgccat gatcacgcac gctggacggt tgcttgagct
     9841 gccgaatgca actgcgttgc ctggcgacac gctgtacgca gctggcatct cgatgttggg
     9901 catgctgtca cgtgaggaac cggagcgggc gctggccgtc aaaaaaagac tcaccagaga
     9961 gttattgcca aacgtccccg tgccgttcga aattctgtgg atggaagcgc tcgcgcagcg
    10021 cagccaaatg cggtgagtat tgagggatcg ctttgctgac tgattttttc ctgagactgc
    10081 gcaccggtgg cataccggtt tcagtgaagg aataccttac cctgatggag gcgctgcaga
    10141 acaagctcag cgccggcagc ctggatgagt tttattacct ggcgcgggct tgcctggtga
    10201 aggacgagtc gaactacgac aagtttgatc gcgtattcgg cgcgtatttc aaggggcttg
    10261 tgacgctgcc cgacaacggg attgtggacc tccctgaaga atggctgcgc aagcaggccg
    10321 agctgctgct gactgaggaa gaaaagaaac ttgtcgaatc actcggcggc tgggaaaaat
    10381 tgatggagac gctgcgcaaa cgtctggagg agcagcgggg ccggcatcag ggcggatcca
    10441 aatggatcgg cactggcggc acttcgccct ttggcgccta cggctacaac ccggaaggcg
    10501 tgcgcatcgg ccaggacggc tcgcgcaacc gcagtgccgt caaggtctgg gacaggcggg
    10561 aataccgcaa tctcgatgat tcgctggagc tgggcgtgcg caacatcaaa gtcgcactga
    10621 agcggctgcg caaattcgcg cgtgaaggcg cggccgaaga gctcgacctg gatagcacca
    10681 ttcgctcaac cgcgaagaat gccggctacc tcgacatcaa gatggtgccc gagcggcaca
    10741 acgcggtgaa ggtgctgctg ttcctggacg tgggcgggtc aatggaaggc tacgtcaagg
    10801 tctgcgaaga gctgttttca gcggcgcgcg tggaattcaa gcacctggaa tacttctact
//
LOCUS       DATMZQ010000191         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2910639, whole genome shotgun sequence.
ACCESSION   DATMZQ010000191 DATMZQ010000000
VERSION     DATMZQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910639"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1305)
                     /gene="hisD"
                     /locus_tag="VN496_06165"
     CDS             complement(64..1305)
                     /gene="hisD"
                     /locus_tag="VN496_06165"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXF16568.1"
                     /translation="MRILERRAAENYVRKLERRGQKLDALEPRVRRIVQNVRRDGDGA
                     LRRYAQTWDGLGVGDPLRVSEHEMQAALTASSPELRVALETAAENIRTFCEWQKPANW
                     EHVRQGRKLGQLVRPLSSIGCYVPGGRYPLPSTLLMTVIPAQVAGVKDIRVVSPRPAP
                     ATLAAAALLGVCEFYRIGGAQAIAALAYGTKSVPRVNKIVGPGNLFVTTAKKIVAFDC
                     AIDFLAGPTEGLILCDEGEPAFIASDLVAQAEHDPDAIVLLVTKSRKFADTVSRSVVA
                     LARGNPIAQQSLRKHGAVLLAASSPQAIEWTNRIAPEHLTTTRERLPGIVSAGSIFLG
                     GYSAQAAGDYGSGPNHVLPTGGIARFRGGLSVLDFVKVMTVQEISRTGLRKIAPSILA
                     LARAEGLRAHARSTELRCGHA"
     gene            complement(1305..>2138)
                     /gene="hisG"
                     /locus_tag="VN496_06170"
     CDS             complement(1305..>2138)
                     /gene="hisG"
                     /locus_tag="VN496_06170"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXF16569.1"
                     /translation="QEATLQLFARAGLNVYTNGRSYSASTADPDVECMLIRAQEMARY
                     VEHGVLDAGLTGLDWVMESGLEVVTVADLVYAKQSRGKVRWVLAVPEVSEYQRAEDLT
                     SCIVATELVNVTRNYFDKKAVSVRVEFSWGATEVKPPTLADAIVEVTETGSSLRANHL
                     RIIDTLLESNTQVIANTSAWKDAGKRRKLENLAVMLRGAMEAQGRVGLMLNVRKSDLA
                     AVLGVLPALQKPTVATLSDDAWVAVNTIIEESAAWEIIPRLKAANAEGIVEYPLNKVV
                     M"
BASE COUNT          433 a          690 c          589 g          426 t
ORIGIN      
        1 agtccagatc gatcgcccaa gggcggctgg tactgcttca attttcgtac tgccgggcgc
       61 gcttcaagca tggccgcacc tcaactctgt ggagcgagca tgagcacgaa gcccctcggc
      121 ccgtgccaga gccagaatag acggagcaat ctttcgcaag cccgtgcgtg agatttcctg
      181 gactgtcatc accttgacga agtcgagcac gctcaatccc ccgcggaagc gtgcgatgcc
      241 tccggtcggc aatacgtgat ttggcccgga tccatagtcc cccgcggcct gcgccgaata
      301 ccctccgaga aaaattgacc cggcactaac gatgccaggc agcctttcgc gagtggtcgt
      361 caggtgttcg ggtgcaatgc ggtttgtcca ctcaatcgcc tggggtgaag acgcggccag
      421 gagcactgcg ccatgttttc ggagagactg ttgggcaatc ggatttccac gggccaacgc
      481 aaccaccgat cgcgaaaccg tgtcggcgaa tttccgcgat ttcgtgacca gcagtacgat
      541 ggcatccgga tcgtgctccg cttgtgcgac caaatccgac gcgatgaacg cgggctcgcc
      601 ttcgtcacaa agaatcaagc cctcggtcgg gcctgccaga aagtcgatgg cgcaatcaaa
      661 ggccacaatt ttcttggccg tggtcacgaa caaattcccc ggccccacga tcttgttgac
      721 tcggggaacg cttttcgtcc cgtaagccag cgccgcaatc gcctgcgcgc cgccgatacg
      781 atagaactca cacactccca acagcgctgc tgccgccaga gtcgccggag caggacgagg
      841 cgagaccact cgaatatctt tcactcccgc gacctgcgcc ggaatcaccg tcatcaacaa
      901 agttgaaggc agaggatagc gtccgccagg cacataacat ccgattgagc tgagcggccg
      961 gaccaattgc cccaactttc gtccctgcct tacgtgttcc cagttcgcag gtttttgcca
     1021 ctcgcagaaa gtccgaatat tttctgctgc ggtttcgagc gccactcgca actccggcga
     1081 tgaagccgtc agcgcggctt gcatctcgtg ttccgaaacg cgcaatggat cgccaacgcc
     1141 cagaccatcc cacgtttgcg cgtatcgccg caaagccccg tccccgtcgc ggcgcacatt
     1201 ctgcacgatg cggcggacgc gcggctccag ggcgtcgagc ttctgtccgc gtcgctccag
     1261 tttccgcaca taattttccg cagcacgacg ttcgagaatc cgcatcacat gaccaccttg
     1321 ttcagcggat attcgacaat gccttcagcg tttgcggctt tcagccgggg aataatctcc
     1381 caagccgccg actcttcgat gatggtgttc accgcgaccc aggcgtcatc gctcagcgtg
     1441 gccacggttg gcttctgcaa tgccggcaga acgccgagca ccgcggccag gtccgacttg
     1501 cggacgttca gcatcaggcc gacacggccc tgggcctcca tcgcaccgcg cagcatcact
     1561 gccaggtttt cgagcttgcg gcgcttgcca gcatctttcc aagcagaagt gttggcaatg
     1621 acttgggtat tggattcgag cagggtgtca ataatgcgga gatggttggc tcgcagcgaa
     1681 ctgccagtct cggtgacttc tacgatggca tcggccagcg tcggaggctt cacctcggtc
     1741 gctccccagg aaaactcaac gcgtacgctg acagctttct tgtcaaaata atttcgcgtc
     1801 acgttcacaa gttcagtggc gacaatgcag cttgtaagat cttctgcgcg ctggtactcg
     1861 gaaacctcgg gcacagccaa aacccaacgt actttgcctc gactctgctt ggcgtagacc
     1921 aggtcggcca cggtcacaac ctcgagcccg ctctccatca cccagtcgag cccggtgagt
     1981 cccgcatcaa gcacgccgtg ctccacgtag cgagccattt cctgggcacg aatgagcatg
     2041 cactcgacat cgggatcagc ggtgctcgcg gagtacgacc ggccgttggt gtagacgttg
     2101 agtccggcac gcgcaaacag ctgtagcgtc gcctcctg
//
LOCUS       DATMZQ010000192         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3947751, whole genome shotgun sequence.
ACCESSION   DATMZQ010000192 DATMZQ010000000
VERSION     DATMZQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3947751"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VN496_06175"
     CDS             complement(<1..1455)
                     /locus_tag="VN496_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HXF16570.1"
                     /translation="MNLLSEAAIFLAAAVVTVPLFRRFKLGAVLGYLSAGVLIGPYGL
                     RFMSAGDNLLHFAELGVVLLLFVIGLELQPSRLWVLRRSVFGLGSAQVLFTGAALALI
                     AFAFGLGLTSALVIGLGLAMSSTAFVLQILAEKGQLSQRHGRSSFAILLFQDLAVIPL
                     LALIPLLGTGDASAVGGSVWLGALKAIGVILAVIIGGRYLLRPVLHAIAAAGIQEIFT
                     AAALLVVIGTSLLMTSVGLSMSLGAFLAGVLLADSEYRHELEADIEPFKGLLLGLFFI
                     SVGMSANLSLVLAHPLAIFGATLALMTIKASILLGLGRMSGHSWDSSRGLAVALCQGG
                     EFAFVLFGLAADNGILGATLTGSLVIVVTLSMALTPLAFVLNDVFIARWLKKRHEEHF
                     DEIDAGENKIIIAGFGRVGQIVARLLRAKKISFTALDKSAEQIDTVRRFGSRAYYGDA
                     SRLDMLRAAKADKAEILVLAIDDIEASLHTVETVR"
     gene            complement(1683..2030)
                     /locus_tag="VN496_06180"
     CDS             complement(1683..2030)
                     /locus_tag="VN496_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXF16571.1"
                     /translation="MSIKRLHVGARLSQVAIHGDTVYTAGIVADDTNVDVAGQTGQIL
                     DKIDQYLKEAGTDKTKIISTTIWLADIKDFAQMNSVWDQWVPKGNTPPRACVESRLAT
                     PSYKVEIRVIAAR"
     gene            complement(2118..>2325)
                     /locus_tag="VN496_06185"
     CDS             complement(2118..>2325)
                     /locus_tag="VN496_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002122354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HXF16572.1"
                     /translation="KLLVTGEAEKIVGCHIIGPGADEIMQGFAVALHMGATKHDFDET
                     VAIHPTVGEELVTMRASRPAVDAA"
BASE COUNT          510 a          697 c          669 g          449 t
ORIGIN      
        1 tcgcaccgtt tcgacggtgt gcagcgaagc ttcgatgtca tcgatcgcga gcaccagaat
       61 ttccgccttg tcggctttgg cggcacggag catatccagg cgcgatgcat cgccgtagta
      121 tgcgcggctg ccaaaacggc gcacggtatc gatctgctcg gcgctcttgt ccagtgccgt
      181 gaaggaaatt tttttcgcgc gcaacagccg tgccacaatc tggccgacgc gcccgaaccc
      241 ggcaatgatg atcttgttct cgccggcatc gatctcatca aaatgttctt cgtggcgttt
      301 cttcagccag cgggcgatga acacgtcgtt tagcacgaat gcaagcgggg tcagtgccat
      361 ggacagcgtg accacgatga cgagtgagcc cgtcagcgtc gcaccgagga tgccgttgtc
      421 ggcagcgagc ccgaacagca cgaaagcgaa ttcaccgccc tggcagagcg caaccgccag
      481 gccgcgggaa gaatcccagc tgtggcccga catgcgtcca agtcccagca ggattgaggc
      541 tttgatggtc atcaatgcca gcgtggcgcc gaagatggca agcgggtgcg ccagcacaag
      601 gctcaggttg gccgacatgc ccaccgagat gaagaacaat ccgagcagca atcctttgaa
      661 cggttcaatg tcggcttcga gttcatggcg gtattccgag tctgcgagca gcacgccggc
      721 gagaaaagca cccagcgaca tggagagtcc cacggaggtc atcagcagcg aggtgccgat
      781 gacgaccagc aacgcggcgg ctgtaaatat ttcctggatg ccggccgctg cgatggcatg
      841 cagcacgggg cgcaacaaat aacgcccgcc gatgatcacg gcgagaatga caccaatggc
      901 cttcaatgca ccgagccaca cgctgccgcc cacggcgctt gcgtctcctg tgccaagcag
      961 ggggatcaaa gcaagcagcg gaataaccgc aagatcctgg aacaacagga tggcaaatga
     1021 cgatcttcca tggcgttgcg aaagctggcc tttttccgca aggatttgca atacgaatgc
     1081 ggtggacgac atcgcgaggc caagaccgat gaccagcgcg ctcgtcaacc cgagtccgaa
     1141 tgcaaacgcg atcagtgcga gcgccgcgcc ggtgaagagt acctgggcag aacccagccc
     1201 gaacaccgaa cggcgcagca cccacagccg cgacggttga agttccaggc cgataacgaa
     1261 cagcaacagc acgacgccca attccgcgaa gtgcagcagg ttgtcgcctg cgctcatgaa
     1321 ccgcaatccg tacggcccga tcagcacacc ggccgagagg taacccaata cggcgcccag
     1381 tttaaaacgg cggaacagcg gcaccgtgac aaccgcggcg gctaaaaata tggcggcttc
     1441 actgagcaga ttcacttcgt cctccgatcc cgcggctctc ctttgacgga cggagaggga
     1501 acttggtcat gcgttggaaa tggagcagaa agtgtactgc attgcttatg gcaaggagca
     1561 gactgtgccc gatgccccgt gatgaactac tggaagctcg ctctccacat tttctgcgac
     1621 ttcaggctaa ccgcatggat acccgtttcg agcataggat tagcgcgcag gtgcgcctcg
     1681 gcttaccgtg ccgcgatgac gcggatttca actttgtatg acggtgttgc gagcctggat
     1741 tctacgcacg cgcgcggcgg cgtattgccc ttcggcaccc attggtccca caccgaattc
     1801 atttgcgcaa aatctttgat atccgcaagc cagatggtgg tcgagattat tttggtcttg
     1861 tctgtgccag cttccttcag gtactggtcg atcttgtcca gaatttgacc tgtctggccg
     1921 gcgacatcga cattggtatc gtctgcgacg atgcccgcgg tatagaccgt atcgccgtgg
     1981 attgcgacct gcgacagacg tgcgcctacg tgcagccgtt tgatgctcat gccattcctt
     2041 ccttgtcaaa tgttgaaccg cgccgatttc acgccgggcg aagataccac gatttgcgcg
     2101 agcggccgac gattgtccta tgcagcatcc acggccgggc ggctggcgcg catggttacc
     2161 aattcctccc ccaccgtcgg atgaatggcg accgtctcat cgaaatcatg cttggttgcg
     2221 cccatatgca gtgcgacggc gaagccttgc atgatttcat ccgcgcccgg accaatgatg
     2281 tggcaaccca ctatcttttc tgcttcgccc gtcaccaaca gcttg
//
LOCUS       DATMZQ010000193         2746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2912352, whole genome shotgun sequence.
ACCESSION   DATMZQ010000193 DATMZQ010000000
VERSION     DATMZQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912352"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VN496_06190"
     CDS             complement(<1..2079)
                     /locus_tag="VN496_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXF16573.1"
                     /translation="MQDSNEEELLRSVALQNARSIFIARQRTEEVSAQAQEQLREAAS
                     RLQLALAAGQLGDWSWDASADLVTFGPRAAEIFGLPPGKPVSRARIRESLHEDDADRA
                     RQMIDRALVEHTVYSAEYRVRHPVRGLRWVAARGLGTYGPDGSITGMIGVFQDITDRK
                     CAEEALRITQTRLQMAIEAGQMGHWEWVLSSGEVHWSPALEKIHGLVPGTFGGTFEDY
                     KRDIHPDDLTRVLANIQGSAQNGSDYRIEYRIIKPDGNMTWIEARGKLFLDAHGNPER
                     MAGICMDATARKQAEEKLQDETRMLELLNQTGTVLTTKLDLQALLQAVTDAATQLSGA
                     QFGAFLYNTTNEKGDPCSLQTVCGAPLEAIAQHRATPRFGPAFTGEGSVRCDDVLSDR
                     RYGNLELYHGMPDARVPVRSYLRVPVIMNSGEIIGGLCFGHSETGVFTERTERLILGI
                     AAQAAVAIENARLYEATQKASEDRQNLLESERAAHADSERANELKDEFLATLSHELRT
                     PLNSILGWAQVLKISATTPDLQRGLDAIERNARLQAQLIEDLLDMSRITSGKMRLDIQ
                     PVELIPVIEAAIETIRPAAEAKLIRIEKLFDGRADAVFGDSSRLQQVIWNLVSNAIKF
                     TPKGGKVQILLEQVNSHIEISVADTGIGIKPEFLPYVFERFRQADATTTRAFGGLGLG
                     LAIVKHLVVLH"
     gene            complement(2079..>2746)
                     /locus_tag="VN496_06195"
     CDS             complement(2079..>2746)
                     /locus_tag="VN496_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HXF16574.1"
                     /translation="SLMYPADGIPGYSRDQFIQDLLLEHETEIRRCLKKGAHKVQIDF
                     TEGRLAMKVDPSGQLLSSFIDLNNMALSRFSIDDRQRLGVHTCPGADRDSTHSADVDY
                     AELLPSLFELKVGNFYVALAGERNRERVLKIVREYRKPDQHVFIGVVAPIDPRVETPE
                     EIRDRALQAAQYIPLDRLGTTDDCGYSPFSDDTSTTRDTAFAKIRARVLGTALAAQKL
                     GAG"
BASE COUNT          499 a          848 c          797 g          602 t
ORIGIN      
        1 gtgcaacacc acgaggtgct tcacaatggc gaggccgagg cccagtccgc caaatgcacg
       61 ggtggtggtg gcgtcggctt gacgaaaacg ctcgaaaaca tagggcagaa actcgggttt
      121 gatgccgatc ccggtatccg ccacgctgat ctcaatgtgc gagttcacct gttcgagcag
      181 tatctgaacc ttgccgccct tgggggtgaa cttgattgca ttggagacca ggttccagat
      241 aacttgctgc agccggctcg aatcgccaaa tacggcatcg gcgcgcccat cgaacagctt
      301 ttcgattcga atcagtttcg cctctgccgc cgggcggatg gtttcgatgg ccgcttcgat
      361 gacggggatc agttccaccg gctgtatgtc gagccgcatt tttccggaag tgatgcggct
      421 catgtcgagc agatcttcga tcagctgcgc ctgcaggcgc gcgttgcgct cgatcgcatc
      481 caatccgcgc tgaaggtcgg gcgtcgtcgc gcttattttc aggacctgcg cccagcccag
      541 gatcgaattc aggggcgtac gcagttcatg tgacagcgtg gcaagaaact catccttgag
      601 ttcattggcg cgctctgaat ccgcgtgtgc tgcgcgttcg ctttcaagca gattctgccg
      661 gtcctccgaa gctttctgcg tcgcctcgta aaggcgtgcg ttttcgatgg cgacggccgc
      721 ctgcgcggca atgccgagga tgaggcgctc ggtcctctcg gtgaaaacgc cggtttcgga
      781 atgcccgaaa cacagcccgc cgatgatttc acccgaattc atgatcaccg gcacccgcag
      841 ataactgcgc acgggcaccc gtgcgtccgg catgccgtga tacagctcaa gatttccgta
      901 gcgccgatcg gacagaacat cgtcgcaacg gaccgaccct tcaccggtga aagcgggacc
      961 gaaccgcggc gtcgcacggt gctgcgcgat ggcttcgagc ggcgcaccgc aaacggtctg
     1021 caagctgcaa gggtcgcctt tttcattcgt cgtgttgtag aggaatgcgc cgaattgcgc
     1081 cccgctcaat tgcgtggcgg catcggtcac tgcctgcagc agcgcctgca aatcgagctt
     1141 ggtggtcagc accgtcccgg tctggttcag cagctccagc attcgcgttt catcctggag
     1201 tttttcttct gcctgcttgc gcgctgtcgc atccatgcag atgccggcca tgcgctcggg
     1261 attgccatgt gcatccagaa acagttttcc gcgcgcctca atccatgtca tgttgccgtc
     1321 gggcttgatg atgcgatatt caatgcgata gtccgatccg ttttgcgcgc tgccctgaat
     1381 gtttgccagc accctggtga gatcgtccgg atggatatcg cgcttgtagt cctcaaaggt
     1441 cccgccgaat gtgccgggca ccaatccatg gattttttcg agcgccggtg accaatgcac
     1501 ctccccggag gaaagcaccc attcccaatg gcccatctgc ccggcttcga ttgccatctg
     1561 caggcgcgtc tgcgtaattc gcaacgcctc ttcggcgcac ttgcgatccg tgatgtcctg
     1621 aaagacgcca atcattcccg tgatcgagcc gtccggcccg tatgtgccca ggcccctggc
     1681 tgcaacccag cgcaggccgc gcaccggatg ccggacccgg tattccgcgc tgtagaccgt
     1741 gtgttcgacc agcgcgcgat cgatcatctg tcgggcgcga tcggcgtcat cttcatgaag
     1801 cgattcgcgt atgcgtgcgc gactgactgg cttgcccgga ggcagcccga atatttccgc
     1861 agcgcgcggg ccgaacgtga ccaggtctgc gctggcgtcc caactccagt caccaagctg
     1921 accagccgcc agcgccaatt gcaggcgact ggctgcttcg cgcaactgtt cctgtgcctg
     1981 tgcgcttacc tcctcggtac gctgccgggc aatgaaaatg cttctggcgt tctgcagcgc
     2041 cactgaacgc aacagctctt cttcgttgct gtcttgcatc accctgctcc caacttttgc
     2101 gcggccagtg cggtacccag cacgcgtgcc ctgattttgg cgaacgccgt atcgcgcgtc
     2161 gtcgatgtgt catcgctgaa cggcgaatat ccgcagtcgt cggtagtgcc gagtctgtcc
     2221 agcggaatgt actgtgccgc ctgcagcgcc cgatcgcgaa tttcttccgg ggtttccaca
     2281 cgcggatcga tcggtgcaac cacgccgata aagacatgct gatcgggctt ccggtattcg
     2341 cggactattt tgagcacgcg ctcgcggttg cgttcgcccg caagcgctac gtagaaattg
     2401 ccgactttga gttcaaacag actcggcagc agttcggcgt agtcgacgtc cgcgctatgg
     2461 gttgaatcac ggtctgcgcc cgggcaggtg tgcacgccga gccgctggcg atcgtcgatt
     2521 gaaaaacgcg acagtgccat gttgttcaga tcaatgaagc tggacagcaa ttgcccggaa
     2581 ggatcaactt tcatggccag gcgcccttcc gtgaagtcga tctgcacctt atgcgcgccc
     2641 ttcttcaaac aacggcgtat ctcggtttcg tgttcgagca gcaggtcctg gatgaactgg
     2701 tcgcgagaat atcccggaat accatccgcc gggtacatga ggctca
//
LOCUS       DATMZQ010000194         5585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4910383, whole genome shotgun sequence.
ACCESSION   DATMZQ010000194 DATMZQ010000000
VERSION     DATMZQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4910383"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1166
                     /locus_tag="VN496_06200"
     CDS             225..1166
                     /locus_tag="VN496_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXF16575.1"
                     /translation="MPRRNEYDVTDRINTTDPASVNGEVNRIFLELYPNASTHLLNRV
                     FRDLARLLAGDYPGYHACDTSYHNLQHSLDVTLAMARLMDGYERTRERRAELGARLFR
                     FGIVTALFHDIGYLRKVNDTRHKNGAEYTLKHVSRGAKFLEDYMNRIGMAELAPIASR
                     IIHFTGYEKPIEKIDVPNISFRLIGSMLGTADIIAQMADRCYLEKCRDRLYPEFVEGG
                     LAARNDADHRTSVMFRSVEDLMLSTPRFYKSARARLNEQLGGAHGYAGKHFGGQNLYL
                     DEIDKNMQYASSVADRRDLSLFRREPPKLSEETLAIV"
     gene            complement(1163..2152)
                     /locus_tag="VN496_06205"
     CDS             complement(1163..2152)
                     /locus_tag="VN496_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXF16576.1"
                     /translation="MRAIEISTPGGPEVLRPGRRPVPSPAVDDVLIEVRAAGVNRPDV
                     LQRKGGYPPPPGASDIPGLEVAGKVVALGQNVKDWKIGDEVCALVTGGGYAEYVTAPA
                     VQCLPVPAGLTLEQAASLPETFFTVWVNVFQRAALKNQETLLVQGGSSGIGVTAIQMG
                     RAFGHQVFVTAGTADKCAECEKLGATRAINYRTEDFVAVIKQLTGGRGVDVILDMVGG
                     DYVPRELTCLAEDGRLSLIAFLGGTKTTLDMTDILRRRLTITGSTLRPRPVEVKASIA
                     QALRQKVWPQIEAGKIRPVIYRTFGLEQAAAAHALMESSEHVGKIMLSVQQQR"
     gene            2302..3312
                     /locus_tag="VN496_06210"
     CDS             2302..3312
                     /locus_tag="VN496_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF16577.1"
                     /translation="MEYRKLGNSGLKVSPLCLGAMMFGDRTDEKEAGRIIGSAFDAGV
                     NFLDTADQYAKGESERITGRLIASNRDHWVLATKMGNPMGTGPNQIGLGRKWMMQAID
                     GSLKRLGTDFVDIYYLHRDFDDASLEEAVVGMGDIIHAGKVRYFGLSNFRGWRIAEIV
                     RLCRALNVPQPIVCQPYYNAMNRQPEVEILPACRYYGIGVVPYSPLARGVLTGKYKPN
                     EAPPEGTRAGRADKRMMETEFRKESLAIAQKIRAFAEKKGMSAGHFAVNWVLNNAIVT
                     SVIAGPRTLEQWQDYLKALEFKFEPKGEALVDSLVSIGHPSTPGYNDPQYPLTGRVPV
                     IH"
     gene            3320..4078
                     /locus_tag="VN496_06215"
     CDS             3320..4078
                     /locus_tag="VN496_06215"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16578.1"
                     /translation="METSAVALVTGGSRGIGRAIVELLAARGYRVMFTYASRAADAAT
                     VEAAIHKTGAQASSMRADVAEVQSAKNVVQSTLAKFGRIDVLVNNAGTHLPDVAIADT
                     SAEEWDRVLQTNLYGPFHMVQAVLPHMRARKSGNIVNLSSNITNRFPARNAAYTVSKV
                     GLEAFTRILSKEEGRNGIRVNAVGPGPIRTDMLEETMVKIGPERADAFIKSVPLGRKG
                     EPREIAEAVAFLVSDAASYMTGQIVFVNGGGPGG"
     gene            complement(4107..4541)
                     /locus_tag="VN496_06220"
     CDS             complement(4107..4541)
                     /locus_tag="VN496_06220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXF16579.1"
                     /translation="MNRPKPELRHTYRHFQAIPTRWADNDVYGHVNNVVYYSYFDTVV
                     NQYLIEQRVLDVPTSTVIGLVVETQCEYFAPLSFPDVVHAGLRVAKLGNSSVRYEIGL
                     FRNDENEASAQGYFVHVYVDRASRNPIAVPSGMRLVLQKLLT"
     gene            complement(4555..5466)
                     /locus_tag="VN496_06225"
     CDS             complement(4555..5466)
                     /locus_tag="VN496_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF16580.1"
                     /translation="MTTTEAQHHRRTLRGIALIMAAVFMFSAMDTLAKHMLKSYPMSA
                     LMWARYMVHIVVMAVLLGPRMGMKLLRTSHLWLQMLRGVLLLASSIFFYFALRYLPLA
                     EAAAISFVGPALTALLSGPMLGDKVSQRQWFAVLLGFTGVLIIMRPGGGVVSLAAVFP
                     LATAVLFSVYQIVTRKLSGREHPYTTLFYTAVVGAVITSVAVPLHWVTPTLVQAVFVV
                     CIGLLGGLGHLLLIRAMEHTSPSTLAPFVYSQLIWSTLLAYLAFGDFPEPMTLVGMVV
                     VVAAGLLAVNWKHMRRVSDTSDQAGTH"
BASE COUNT         1222 a         1625 c         1616 g         1122 t
ORIGIN      
        1 aatgaacccg actggcgcca ggttggtttg cctttcaacc agtcgatcaa tgcatcacct
       61 tttacctgtg ggcgatcgaa gggccatgac ggaaattctt gtttcaggca cgttttgctc
      121 tagaaatagt gcgcaattcg aatttcggct tgaacatacc gctggcggga gggtaccatg
      181 ggtccatatt ccaactattt gaacgggcag gaggatattg atccatgccg cgtcgcaatg
      241 agtatgatgt aacagacagg atcaatacga ccgatcctgc ctcggtcaat ggggaggtca
      301 atcgcatctt cctcgagttg tatccaaatg cgtcgacgca tctcctgaac cgtgtttttc
      361 gtgacttggc acgtctgctc gcgggagact atcccggcta tcacgcctgc gacaccagct
      421 accacaacct gcagcattct ctcgacgtca cgctcgcaat ggcgcgactc atggacggtt
      481 acgaacgtac ccgggaacgc cgcgcggaac tcggtgcgcg cctgttccgt ttcggcatag
      541 tcaccgcgct atttcatgac atagggtatc tgcgcaaggt aaacgatacc cggcacaaga
      601 acggcgcgga atatacgctg aagcacgttt cccgcggtgc gaagttcctg gaggattaca
      661 tgaaccgcat cggcatggcc gaactggcac cgatcgcatc gcgaatcatc catttcacgg
      721 gttacgagaa gccaatcgag aaaatagatg tgccaaatat ctcattccgg ctgattggca
      781 gcatgcttgg cacggccgat atcattgcgc agatggctga tcgctgttat ctcgagaagt
      841 gccgtgaccg cctgtacccg gagtttgtcg aaggtggact ggctgcgcgc aatgatgccg
      901 atcaccgtac cagcgtaatg ttccggtccg tggaagacct gatgctttcg acgccgcgtt
      961 tttacaaaag cgcgcgggcg cgcctgaatg agcagctggg cggcgcgcat ggctatgccg
     1021 gcaagcattt cggcggtcaa aatctttacc tcgatgagat cgacaagaat atgcaatacg
     1081 cttcctccgt ggcggatcgg cgcgacctgt ccctgttccg ccgcgagccg ccaaaactca
     1141 gcgaagagac attggccatc gtttagcgct gctgctgtac actcagcatg attttcccca
     1201 catgctcgct cgattccatg agcgcatgcg cggctgcagc ctgttccagt ccgaaagtcc
     1261 gatagatcac cggtcgaatt tttcccgcct cgatttgcgg ccataccttt tggcgcaacg
     1321 cctgcgcgat gctggcctta acctccaccg ggcgcgggcg taacgtcgat cccgttatag
     1381 tcagacggcg gcgcagtatg tcggtcatgt cgagcgtggt tttggtgccg ccgaggaaag
     1441 cgatcaaaga cagccggcca tcctcggcga gacaagtcaa ttcgcgcggt acgtaatcgc
     1501 cgccgaccat gtcgaggatt acatctacgc cgcgaccccc agtgagctgt ttgataactg
     1561 ccacgaaatc ctcggtgcga tagttgattg cacgcgttgc accgagcttt tcacattccg
     1621 cgcatttgtc cgccgttccc gcagtcacga aaacctggtg tccgaacgcg cgtcccatct
     1681 ggattgccgt gacgccgatc ccgctcgagc ctccttgcac cagcagcgtt tcctgatttt
     1741 tcagtgcagc gcgctggaac acgttgaccc acactgtgaa aaacgtctcc ggcaggcttg
     1801 cggcctgctc gagagtcagt cccgccggca cgggaaggca ttgcacggcc ggcgcggtga
     1861 catactccgc atagccgccg ccggtgacca gcgcacagac ctcatcgccg attttccagt
     1921 ccttcacgtt ttgaccgagc gctacgacct tgcccgccac ttccaaccca ggtatgtccg
     1981 acgcgccggg cggcggcggg tagccgcctt tgcgttgcag cacatccgga cggttcactc
     2041 cggcagctcg aacttcaatc aacacgtcat cgacagccgg tgacggcacg ggacggcgtc
     2101 ctggacgcaa tacttccggg ccaccaggag tcgaaatctc gatggcgcgc atatgggttg
     2161 gcaaaggttt ggacatgacg agcctcttcg ttggagctct gtttatatca ttttgcgcgc
     2221 catgccttgc agagtttgcg acttgcccgg gcttgcggca tcattccgcg ctcttcaaga
     2281 ataatgacgt ggggggatgt catggaatat cgcaagctcg gcaacagcgg actgaaagtt
     2341 tcgcccctat gcctgggcgc catgatgttc ggggatcgca cggatgagaa agaagcgggc
     2401 cgcatcatcg gttcggcttt cgatgccggc gtgaattttc tggataccgc agaccagtac
     2461 gcgaaaggcg agtccgagcg catcactggc agattgatcg catcgaaccg ggaccattgg
     2521 gtgctcgcta cgaaaatggg taacccgatg ggcacgggac cgaatcagat cggcctcgga
     2581 cgcaagtgga tgatgcaggc catcgatggc agcctgaagc gcctcggtac agacttcgtg
     2641 gatatctatt atctgcatcg cgatttcgat gatgcttccc tggaggaagc agtcgtcggc
     2701 atgggagaca tcattcatgc gggcaaggtc cgctacttcg gattgtcaaa cttccgcggc
     2761 tggcgcatcg ccgaaatcgt ccggctgtgc cgcgcactca atgtgccgca gccaatcgta
     2821 tgccagcctt attacaacgc catgaaccgg cagccggaag tcgaaatcct gccggcctgc
     2881 cgctactacg ggatcggcgt cgtgccgtac agcccgcttg cgcgcggggt gctgactggc
     2941 aaatacaaac cgaacgaggc accgccagaa ggaacgcgcg cgggacgcgc tgacaagcgc
     3001 atgatggaaa ccgaattccg caaagagtct ttggcgatcg cgcaaaaaat aagagctttt
     3061 gccgaaaaga aaggcatgag cgctggacac tttgcagtca attgggtgct gaacaatgcg
     3121 attgtcacca gcgttattgc tggaccacgc accctggagc aatggcagga ttatctcaag
     3181 gcgctggaat tcaagttcga accgaaagga gaagcgttgg ttgattcact agtgtccatc
     3241 gggcatccct cgacgcccgg gtacaacgat ccacaatatc cattgaccgg ccgcgtgccg
     3301 gtcattcact gagccaacca tggaaacttc tgcggttgct ctggtcacgg gcggttcgcg
     3361 cggcatcggc cgcgccatcg tcgagttgtt ggccgcgcgc ggttatcgtg tgatgtttac
     3421 ctatgcgagc cgcgcagccg acgcagccac ggtggaggct gcaattcaca agaccggcgc
     3481 acaagcgagc agcatgcgcg ccgatgtcgc ggaggttcaa tctgcaaaga acgtcgtgca
     3541 atctacgctg gcgaagttcg gccgaatcga cgtgctggtc aacaacgccg gaacgcactt
     3601 gcccgatgtc gccatcgccg atacgtcggc cgaagaatgg gatcgcgtgt tgcagaccaa
     3661 cctttacggc ccgtttcaca tggtgcaggc cgtgcttccg cacatgcgtg cgcgcaagag
     3721 cggcaatatc gtcaaccttt cgtccaacat caccaatcgt ttcccggcgc gcaatgcggc
     3781 ctataccgtg tcaaaagtgg gactggaggc gttcacgcgc atcctctcaa aggaagaagg
     3841 ccgcaacggc atccgcgtca atgcggtggg cccaggaccg atccgcactg acatgctgga
     3901 agaaaccatg gtcaagattg gccccgagcg cgccgacgcc ttcataaaat cggtgccgct
     3961 cgggcgcaaa ggcgagccca gagaaatcgc ggaggccgta gcgttcctgg tttctgatgc
     4021 ggctagctat atgaccggac agatcgtctt cgtcaacggc ggtggcccgg gcggctagct
     4081 tgctacgctg ccgcgctgtg acccgttcag gtcaacagtt tttgcaggac caggcgcatg
     4141 ccagagggca cggcgattgg attacgcgag gctcgatcca cgtacacgtg cacgaagtaa
     4201 ccctgcgcag aggcttcgtt ttcgtcattc ctgaataagc cgatttcata gcgcacgctg
     4261 ctgttgccta gctttgcgac gcgtagtcca gcatggacca cgtccggaaa gcttagcggc
     4321 gcaaagtact cgcattgtgt ctccaccacc aggccgatca ccgtgctggt cggcacatcg
     4381 agaacacgct gctcaatcag atattggttc accaccgtgt cgaaatagct gtaatagacg
     4441 acgttgttta cgtggccata gacgtcgttg tcggcccagc gcgtgggaat cgcctggaaa
     4501 tggcggtagg tatgacgcaa ttccggttta ggtcgattca taggtttacc gttatcagtg
     4561 ggtgccggcc tggtcggaag tgtcactcac ccggcgcatg tgtttccagt tcacagcaag
     4621 caatccggcc gcaacgacta cgaccatgcc gaccaatgtc atcggttccg ggaaatcgcc
     4681 gaatgccaga taagccagaa gcgtggacca gatcagttgc gaatagacga aaggcgccag
     4741 tgtagatggc gaagtgtgct ccatggcgcg aatcagcaac aaatgtccca ggccgcccag
     4801 cagaccgatg catacaacaa acacggcctg gaccagggtc ggggtaaccc aatgcagggg
     4861 caccgcaacg ctggtgataa ccgcgccgac caccgcggta tagaacagcg ttgtataagg
     4921 atgttcgcgg cccgagagtt tgcgcgtgac aatctggtac acgctgaaca agacagcggt
     4981 cgccagcgga aacaccgcag ccagggaaac cacgccgcca cccgggcgca tgatgatcag
     5041 cacgccggtg aatcccagca agacggcgaa ccattgtcgc tggctaacct tgtcgccgag
     5101 catcgggccg gacaacaggg cagtcagtgc cgggcctaca aagctgatgg cagccgcctc
     5161 cgcgaggggc agatagcgca acgcgaaata gaaaaaaatg gagcttgcca acagcagcac
     5221 gccgcgcagc atttgcagcc aaagatgcga tgtccgcagc agtttcatcc ccatgcgcgg
     5281 acccagcagc acggccatca ccacgatgtg gaccatgtag cgcgcccaca tcagcgcgga
     5341 catcggatac gacttcagca tatgcttggc aagcgtatcc atcgccgaga acatgaatac
     5401 cgcggccatg attagagcga tgccgcgcag tgtgcggcgg tggtgctgcg cttccgtggt
     5461 cgtcattgcc ggaaacgcgg acagggggag cgccgtctcc gtcgcgaagc gggtgatgag
     5521 agtaatagtc ctattttttc gtcattcaac attgttacga cattcaagta tgcacgtgct
     5581 tgctg
//
LOCUS       DATMZQ010000195         4904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2567158, whole genome shotgun sequence.
ACCESSION   DATMZQ010000195 DATMZQ010000000
VERSION     DATMZQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567158"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..627)
                     /gene="rdgB"
                     /locus_tag="VN496_06230"
     CDS             complement(34..627)
                     /gene="rdgB"
                     /locus_tag="VN496_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXF16581.1"
                     /translation="MLNKLVIASSNAGKLREIRQILAPLDIETVPQTAFGVAEAEEPY
                     DTFIENALTKARHVAALTGLPALADDSGLCVDALAGAPGVHSARFAGDTRSDERNNQK
                     LLEVLGVEQNRRAHYYCVIVVMRHARDPEPLIAQGVWQGEILFAPRGACGFGYDPLFL
                     DTASGKTGAELEPARKNLISHRGKALALLSEALRQSR"
     gene            complement(648..1382)
                     /gene="rph"
                     /locus_tag="VN496_06235"
     CDS             complement(648..1382)
                     /gene="rph"
                     /locus_tag="VN496_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXF16582.1"
                     /translation="MKEPAMRPSGRASDQLRKLSITRGYTRHAEGSVLIECGDTRVIC
                     TASIDDKVPPFLKGRGQGWLTAEYGMLPRSTNTRSDREAARGKQTGRTQEIQRLIGRS
                     LRAVINLANIGERTIQIDCDVIQADGGTRTASITGAYVALHDAVTVLKNNHAIPNWPL
                     RDFVAAISVGIFQGVALLDLDYAEDSKSDTDMNVVMTGSGGIVEVQGTAEGAPFSRQQ
                     LDEMLALAERGIGQLVDAQKAALKIG"
     gene            1529..2395
                     /locus_tag="VN496_06240"
     CDS             1529..2395
                     /locus_tag="VN496_06240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXF16583.1"
                     /translation="MIFSMTGYAVAAVELPFGALNLELRSVNHRYLDIQFRIPDELRP
                     LESGLRERATARIGRGKVEVRIAISKSAASQSNLQLDERLLAQLVQLDGRVRSTLPGA
                     LSLSVADVLRWPGMLGNDNLVPDELHEVCARLLERALDDLTASRSREGEKLKQLLLER
                     ITSMESLVARLMPRMPQLVVSYREKLAARLKEAMVNLDDERLKQELVLFASKIDVDEE
                     LSRLSAHFGEIRRILDKGGAAGKRLDFLMQELNREANTLGSKSVDMEFTQTSMELKVL
                     IEQMREQIQNIE"
     gene            2392..3000
                     /gene="gmk"
                     /locus_tag="VN496_06245"
     CDS             2392..3000
                     /gene="gmk"
                     /locus_tag="VN496_06245"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXF16584.1"
                     /translation="MKGNIFIVCAPSGAGKTSLVAELLRREHNVRLSVSHTTRLPRPG
                     EQDGRDYHFVSRPVFDSMVAKGAFLESAEVHGNLYGTSHSWIIAQRDRDVDIVLEIDW
                     QGAQQVRKLLPEAVGIFILPPSPDTLRRRLIDRGQDADGVIERRVQAARGEISHLAEF
                     DYVIINNNFDDAVEDLVSIVRATRLRTAAQVSRHSNLINSLK"
     gene            3008..3211
                     /gene="rpoZ"
                     /locus_tag="VN496_06250"
     CDS             3008..3211
                     /gene="rpoZ"
                     /locus_tag="VN496_06250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXF16585.1"
                     /translation="MARITVEDCLKHITNRFELTLAATYRARQLATGASPLVDTNKDK
                     PTVIALREIASEKVGAEILKTKV"
     gene            3285..>4904
                     /locus_tag="VN496_06255"
     CDS             3285..>4904
                     /locus_tag="VN496_06255"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXF16586.1"
                     /translation="MAAAEAPMSEAELLFRNAAGYLKPEDIVQLEDAYQFSETAHEGQ
                     FRKSGDPYISHPIAVAQILAKWHLDAQALTAALLHDVMEDTAVTKAEISEKFGKHVAD
                     LVDGVSKLDRIEFQTQEEAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLTVMVPK
                     KRARIARETLEIYAPIAHRLGLNELYQELQELAFRHLYPNRFNVLSKALLRARGNRRE
                     VVDKILEAIKQRLADSHLEATVTGREKNLYSIYNKMHEKNLSFAEVFDIYGFRVLVKD
                     TAACYLALGALHHLYKPIPGKFKDYIAIPKANGYQSLHTTLFGPFGTPIEVQIRTIVM
                     HKIAEAGVASHWLYKESDVQINELHMKTHQWLQSLLELQSESGDSIEFLEHLKVDLFP
                     DEVYVFTPKGKILSLPHGSTCVDFAYAVHTDIGNRCVAAKVNHELVPLRSELRNGDRV
                     EIITAAHAKPNPAWLSYVVSGKARSHIRHFLKTMQYEESSQLGERLLNQALSALKAQP
                     EHVGESQWEKLLKENSAKSRQQVLADIGLGKQ"
BASE COUNT         1080 a         1408 c         1374 g         1042 t
ORIGIN      
        1 gccaatatgg cattgtcctc gttcgctggt gtgtcagcga ctttgcctga gcgcctcgct
       61 taacagtgcc aatgccttcc cacgatgact tatgagattc tttcgcgcag gctccagttc
      121 cgctcctgtt ttcccggatg cggtatccag gaacaaagga tcgtagccga acccgcaggc
      181 accccgcggt gcgaatagta tctcgccttg ccataccccc tgggcgatca gcggctcagg
      241 gtcccgagca tgacgcatta ctacgatgac gcagtaataa tgcgcccttc tattctgctc
      301 aacacccaat acttcgagca gtttttggtt gttgcgttcg tcagaccggg tgtcaccggc
      361 aaagcgcgcg gaatgcacac ccggcgcgcc ggccaaggca tcgacacaga gacccgaatc
      421 atccgctaat gcgggcagcc ccgtaagggc tgcaacgtgc cgggccttgg tgagtgcgtt
      481 ttcgatgaat gtgtcatacg gttcttccgc ctccgctact ccaaacgccg tctgcgggac
      541 cgtttcaata tccagtggcg caagaatctg gcgtatttcc cgaagcttac cggcgttgct
      601 agatgcgatg accaatttat tcaacatctc cgcacgtaac ttcatttcta gccgattttc
      661 agggcggctt tttgcgcatc caccagttgc ccgataccgc gctcagccaa tgcgagcatt
      721 tcatcgagtt gctggcgcga gaacggagcg ccttcggcag tgccctgcac ttctacaatg
      781 ccaccgctgc cggtcatgac aacattcatg tcagtgtcgc tcttggagtc ctcggcataa
      841 tcaaggtcaa gcaacgctac gccttggaaa atgccgacgg aaatcgcggc aacgaagtcg
      901 cgcaatggcc aattcgggat ggcgtgattg ttcttcagga cagttacggc atcatgcaag
      961 gcgacatatg ccccggttat gcttgcagtc cgcgtgcctc cgtctgcctg gatgacatcg
     1021 cagtcaatct ggatggtccg ctcgccgatg ttcgccagat taattaccgc gcgcaggctg
     1081 cggccgatca ggcgctgtat ttcctgcgtg cggcctgttt gcttgccacg cgcggcttcg
     1141 cgatcggacc gcgtattcgt ggagcgtgga agcatcccgt actccgccgt cagccacccc
     1201 tgtccccggc cttttaaaaa gggcggtacc ttgtcgtcga tgctggccgt acagatgacc
     1261 cgcgtgtcgc cgcattcaat cagcaccgag ccttctgcat ggcgcgtata gccgcgagtg
     1321 atggaaagtt ttcttaattg gtcggaggct cgtccactcg ggcgcatggc tggctctttc
     1381 atatcgctgt tgtcattgga atggacaatg gccgcccaag gggcacgacg gccatgcgtt
     1441 tcagaaaacg ctatgatttg tgtgctatct tttcggctcg ccaattttgg tgaagtctct
     1501 tgcccttgta aagcatccgg accgccaaat gattttcagc atgacagggt atgcagttgc
     1561 ggcggtcgaa cttccgtttg gcgcgctgaa tctcgaactg cgttcggtaa accaccgata
     1621 tcttgatatt cagtttcgca ttccagatga attgcggccg ctcgaatcag ggctgcgtga
     1681 gcgggcgacc gcgcgcatcg gccgcggcaa ggttgaagta cgaattgcaa tcagcaagtc
     1741 tgctgcctcg caatccaatc tgcagctcga cgagcgcttg ctggcgcagc ttgtgcagct
     1801 tgatgggcgt gtgcgatcga cgctgcccgg ggcgttatcg ctttcggtag ccgatgtgct
     1861 gcgctggccc ggcatgctcg gcaacgacaa tctcgtgccg gacgaattac acgaagtgtg
     1921 cgcgcgcctg cttgagcgcg cactcgacga cctgacggct tcccggtcac gcgaagggga
     1981 aaaactcaaa cagctcctgc tggaacgcat cacttcaatg gaatcattgg tggcccgcct
     2041 gatgccccgc atgccgcagc tggtagtgag ctaccgggaa aaactggctg cccggctcaa
     2101 agaggcgatg gtgaacctgg acgacgaacg tctcaagcag gaattggtgc ttttcgcctc
     2161 aaagatagac gtggatgaag aattgtcgcg actaagcgcg cattttggag aaatccgccg
     2221 cattctcgat aaaggcgggg ccgcgggcaa gcggctcgat tttttgatgc aggaacttaa
     2281 tcgtgaagcc aacacgttgg gttcaaaatc agtcgacatg gaattcaccc aaacgtccat
     2341 ggaactcaaa gtgctgatcg agcaaatgcg cgagcagatt caaaacatcg agtgaaagga
     2401 aatatcttca ttgtttgcgc gccttccgga gccggcaaaa cctcgctggt cgccgaattg
     2461 cttcgccggg aacacaacgt gcggctttca gtctcgcata ctacccgctt gcctcgcccg
     2521 ggtgagcaag acggtcgcga ttatcatttt gtgtcgaggc ccgtgttcga ctcgatggtc
     2581 gccaaaggcg cgtttctgga aagcgccgaa gtgcatggca acctgtacgg gacctctcac
     2641 agctggatca tcgcgcagcg ggatcgcgac gtcgatatcg tattggaaat agactggcag
     2701 ggcgcacagc aggtgcgcaa gctgctgccg gaggccgtcg gtatcttcat ccttccacct
     2761 tcaccggaca cgttacgccg gcgtttgata gaccgcgggc aggacgctga cggcgtcata
     2821 gagcgcagag tgcaggctgc gcgtggtgag atctcgcatt tggccgaatt cgactatgtt
     2881 attattaaca acaatttcga cgatgccgtc gaagatctgg tgagtatcgt gcgcgcgacc
     2941 cgtttgcgga ccgccgctca agtttcccgc cacagcaatt taatcaatag cctgaaatga
     3001 gccgcatatg gcgcgcataa ccgtagaaga ctgtctcaaa cacatcacga accgattcga
     3061 gctcacgctg gccgcaacat accgcgcacg ccaactcgcg accggcgctt ctccgctggt
     3121 cgacaccaac aaggacaagc cgacggtcat cgccctgcgg gagattgcct cggaaaaagt
     3181 tggagcggaa attctcaaga ccaaagtctg acgatacggc cgttacgaca cgccaggagg
     3241 ttcagcccac acaacccgat ctgccggtga atgcgctagt ttcgctggct gcggctgaag
     3301 cccccatgtc cgaagccgag cttctgttca ggaatgcagc cggctatctc aagccggagg
     3361 atatcgttca gcttgaggac gcttaccagt tcagcgaaac cgcccacgaa gggcagttcc
     3421 gtaaatcagg cgatccgtat atctcccacc cgatcgctgt agcacaaatc ctggcgaagt
     3481 ggcatctgga tgcgcaagcc ctgactgctg cgctgctgca tgatgtgatg gaggacaccg
     3541 ccgtcaccaa ggccgagatc agcgaaaaat tcggcaagca cgttgccgac ctggtcgatg
     3601 gtgtctcgaa gctcgatcgc atcgaattcc agacgcagga agaggcgcaa gcggaaaact
     3661 tccgcaagat gttgctcgcc atggcgcgcg atgtccgggt aatcctgatc aagctcgcag
     3721 accgcctgca caacatgcgc acgctaaccg tcatggtgcc gaagaaacgc gcgcgcattg
     3781 cacgtgaaac gctggagatc tacgcgccca tcgcgcaccg gctgggactg aatgagcttt
     3841 atcaggaatt gcaggaactc gctttccgcc atctataccc gaatcgcttc aacgtgctgt
     3901 ccaaggcgct gctgcgcgcg cgcggcaatc ggcgggaggt tgtggacaag atactggagg
     3961 ccatcaagca gaggcttgcg gacagtcatc tggaagctac ggtcaccggc cgcgagaaaa
     4021 atctgtacag catctataac aagatgcatg aaaagaacct gtcattcgcc gaagtcttcg
     4081 atatctacgg atttcgcgtg ctggtgaaag acacggcagc gtgttatctc gcattgggcg
     4141 cactgcatca cttgtacaag cccattccgg gaaaattcaa agactacatc gccataccga
     4201 aagccaatgg ctatcagtct ttgcatacca ccctgttcgg gccattcggc acgccgatcg
     4261 aagtgcagat tcgcacgatc gtcatgcata agatcgccga agcgggcgtg gcttcgcact
     4321 ggctgtacaa ggaaagtgac gtccagatca acgaactgca catgaagacg caccagtggc
     4381 tgcagagcct gttggaattg cagtccgagt cgggtgactc catcgaattc ctggaacatc
     4441 tgaaagtcga tctgttcccg gacgaggttt acgtctttac gccgaaaggc aagatacttt
     4501 cgctcccgca cggctccacg tgtgtcgatt ttgcctacgc ggtgcacacc gacattggca
     4561 atcgctgcgt ggcggccaaa gtgaatcatg aactcgtgcc cttacgctct gagctgcgca
     4621 acggtgatcg cgttgaaatc atcaccgccg cgcacgccaa gccgaatccg gcctggttga
     4681 gttacgtggt cagcggaaag gcgcgctcgc atatccggca cttcctcaag accatgcagt
     4741 atgaggaatc ttcacaactg ggcgagcgcc tgcttaatca ggctttgagt gcgctgaaag
     4801 cccagcccga gcatgtcggc gaatcgcaat gggaaaagct gctcaaggag aacagcgcga
     4861 agtcgcggca acaagtgctg gctgatatcg gtctgggcaa gcaa
//
LOCUS       DATMZQ010000196         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3950144, whole genome shotgun sequence.
ACCESSION   DATMZQ010000196 DATMZQ010000000
VERSION     DATMZQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3950144"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VN496_06260"
     CDS             <1..335
                     /locus_tag="VN496_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXF16587.1"
                     /translation="THGGRRSTGLEAIEWGRRMQKAGAGEILLTSMDRDGTKAGFDLE
                     LTRAFSEALEVPIIASGGVGSLQHLVDGIVLGKADAVLAASVFHYGELTVHQAKQFLS
                     QNNIEVRL"
     gene            332..727
                     /gene="hisI"
                     /locus_tag="VN496_06265"
     CDS             332..727
                     /gene="hisI"
                     /locus_tag="VN496_06265"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HXF16588.1"
                     /translation="MSDGWLNEVIFDANGLIPAIAQDKSTGALLMLAWMNREALSETV
                     RSGRAVYWSRSRKRLWRKGEESGHIQLVKQVRIDCDADVIVLEVEQVGGIACHTGHER
                     CFFRKLENGQWVETEPSLKDPGRIYDSKP"
     gene            724..1050
                     /locus_tag="VN496_06270"
     CDS             724..1050
                     /locus_tag="VN496_06270"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HXF16589.1"
                     /translation="MINADILRRLGETIASRKDTDPARSYVAGLFSKGEDAILKKIGE
                     EAAEVLLAAKDGDKLHIVRETADLWFHCLVMLAWHNLGPDDVLAELERREGISGLDEK
                     TARLRL"
     gene            1089..1454
                     /locus_tag="VN496_06275"
     CDS             1089..1454
                     /locus_tag="VN496_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXF16590.1"
                     /translation="MTTTVDNCVFCKIIRGQIPSRKVYEDDDVLAFHDINPVAQVHFM
                     LIPKEHVESLEKVQDRHQLVLGKMLMLASKLAHEQGSPDGFRTVINTGRIGRQDVYHV
                     HMHVLGGNNPLPGMIQRST"
     gene            1480..1704
                     /gene="tatA"
                     /locus_tag="VN496_06280"
     CDS             1480..1704
                     /gene="tatA"
                     /locus_tag="VN496_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HXF16591.1"
                     /translation="MGSFSIWHWLIVLVVVLLIFGTKKLRNMGSDLGGAVKGFKEGMK
                     TADDDKPAARLDGANTGQTFEGEVRDKTKT"
     gene            1732..2208
                     /gene="tatB"
                     /locus_tag="VN496_06285"
     CDS             1732..2208
                     /gene="tatB"
                     /locus_tag="VN496_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HXF16592.1"
                     /translation="MFDIGASEIFVIGVVALIVIGPERLPRVAKTLGHLFGRLQRYVA
                     EVKSDISREIELDELRNLKNTMQDAARSIEQSVASQVNYIDNEVKQAGVEVQKQVDSV
                     QKQAESLVEPVAGIQLIPPATPAPEATGAVDPPQPVDGAPELHSNPGEKSKQEKPG"
     gene            2256..3002
                     /gene="tatC"
                     /locus_tag="VN496_06290"
     CDS             2256..3002
                     /gene="tatC"
                     /locus_tag="VN496_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXF16593.1"
                     /translation="MAEPDSFMSHLIELRDRLLRCVVALLLVFMCLFPWARDLYALLA
                     QPMIASMPAGGQLIATEVTAPFFVPMKVTMMAAFVIALPYLLYQIWAFVAPGLYAHER
                     KLVAPLIITSTLLFICGMAFAYYLVFPVVFHFMVSVAPTGVAVMTDIGKYLDFVLTLF
                     LAFGVTFEVPVAVVILVRMGVVSVAKLKDIRPYMIVGAFVLGAVFTPPDVVSQVMLAV
                     PLWLLYEVGIYVSQWVTKPSTEESETSSTS"
     gene            complement(3027..>3546)
                     /locus_tag="VN496_06295"
     CDS             complement(3027..>3546)
                     /locus_tag="VN496_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXF16594.1"
                     /translation="ENFIQTDAAINPGNSGGALVDAYGNLVGVNSAIYSRSGGSLGIG
                     FAIPANLAKQVMEQIVRTGAVTRGWIGVEVQDISAELAESFKLSNAHGTLIAGVLRGG
                     PADRAGIKPGDILLAVESRDVLDSTSMLNLVAALEPGKAATLKILRNRSEMSVKVNVG
                     KRPRPARRNELE"
BASE COUNT          824 a          897 c          997 g          828 t
ORIGIN      
        1 ttacccacgg cggtcggcgt tcgaccggac tggaggcaat tgaatgggga aggcgaatgc
       61 aaaaagcggg tgccggcgaa atcttgctta cgagtatgga ccgtgatggg actaaggccg
      121 gtttcgacct cgaactaaca cgtgcatttt cggaggccct ggaggtacca atcattgcaa
      181 gtggaggcgt cggtagtctg cagcatctcg ttgatggcat cgtactgggc aaggccgatg
      241 ccgttctggc ggccagcgtg tttcattatg gtgaattaac cgtacaccag gcgaaacaat
      301 tcttgtctca aaataacatc gaggttcgct tgtgagcgac ggctggctga acgaagtgat
      361 atttgacgca aatggactga tcccggccat tgcgcaggac aagagtaccg gagcattgct
      421 gatgctcgca tggatgaacc gcgaagcact ttcagaaaca gtgcgatccg ggcgggcagt
      481 atactggtcc cgttcgcgaa aacgcctgtg gcgcaaaggc gaagaatcgg gtcacataca
      541 actcgtcaag caggttcgaa tagattgcga cgcagatgtg atagtgctgg aagtggaaca
      601 agttggaggc attgcctgcc ataccgggca tgaacgctgt ttttttcgca agctcgaaaa
      661 cggacaatgg gttgaaactg agccttctct gaaagatccg gggcggattt acgattccaa
      721 gccatgataa atgccgatat tctgcgccgc cttggcgaaa cgattgcgtc acgcaaggat
      781 accgatccag cccgctccta cgtggctggc ctgttctcga aaggcgagga cgccattctc
      841 aagaaaatcg gagaagaagc tgccgaggtc ctgcttgccg ccaaggacgg tgataaactt
      901 catatcgttc gtgaaacggc cgacctttgg ttccattgtt tggtgatgct ggcatggcac
      961 aatctcggcc cggatgacgt ccttgccgag cttgagcgac gcgaaggcat ctcaggcctg
     1021 gacgaaaaaa cggctcggct gagattatga tgacctgttg tggaaactga ctgctgtcac
     1081 gatcgccaat gacaacgaca gtcgataact gtgtcttctg caagattatc cggggccaga
     1141 ttccgagccg caaagtctat gaagacgacg atgtactcgc cttccacgac atcaatccgg
     1201 tcgcgcaagt ccacttcatg ctgattccaa aagagcatgt ggaatcgctt gagaaggtgc
     1261 aggaccgtca ccagttggta ttgggcaaaa tgctgatgtt ggcgagcaaa ttagcacacg
     1321 agcaaggttc accggacggg ttcaggaccg ttatcaacac cggccgtata ggccggcaag
     1381 atgtatatca cgtgcatatg catgtgcttg gcggaaacaa tccactgccg gggatgatcc
     1441 aacgctcaac atgaatatct tgatttagga gtactagaga tgggttcatt cagtatatgg
     1501 cactggctta tcgttttagt cgtagtgctt ttgattttcg gaaccaaaaa attgcgcaac
     1561 atgggttctg atctgggcgg agccgtgaaa ggtttcaagg aaggcatgaa gaccgccgat
     1621 gacgacaagc cagccgcaag gctggacggc gccaataccg gacagacgtt cgaaggcgaa
     1681 gtaagggaca agactaaaac ctgagattgc gttctctcgc cgtctgcatt tatgttcgat
     1741 attggcgcat cggagatctt tgtcatcggg gtagtcgcgc tcatcgtcat cggccccgaa
     1801 aggctgccgc gcgttgcgaa gacgctggga cacctgtttg gccggcttca gcgctatgtc
     1861 gccgaagtga agtcggacat cagccgggaa atagagctgg acgaactgcg caaccttaag
     1921 aacacgatgc aggatgctgc ccggtccatc gagcagtcgg tcgcgtcgca ggtaaattac
     1981 attgataacg aggtcaagca ggccggtgtt gaagtgcaga aacaagttga ttcggtgcag
     2041 aagcaagctg agtcgctggt tgagccggtg gcggggattc agttaatccc tcctgcaaca
     2101 ccggcgcccg aagcaaccgg tgccgtggat ccaccacagc cggtcgacgg cgcgccggag
     2161 cttcattcaa atcccggaga gaaatccaag caggaaaagc cgggctgatt taggtaaagc
     2221 catctaccct agtttttgac acgcacaaat cccagatggc cgagccagat tcattcatgt
     2281 cgcatctaat cgagttacgc gatcgattat tgcgttgcgt agttgcattg ctcctggtgt
     2341 tcatgtgttt gtttccgtgg gcgcgagact tgtacgcgtt gctggcgcag ccgatgattg
     2401 cctcaatgcc cgcaggcggt cagctgattg ccactgaggt gacggcgcca ttttttgtgc
     2461 cgatgaaggt cacaatgatg gcagcgtttg taattgcact accttacctg ctataccaga
     2521 tttgggcctt tgtggcgcct ggattgtatg cgcatgaacg aaaactcgtg gcgccgttga
     2581 ttatcaccag cacgctgctg tttatttgcg gtatggcctt cgcatattat ttggtgtttc
     2641 ccgtggtgtt tcattttatg gtttccgttg caccgacggg agttgcagta atgacagaca
     2701 ttggcaagta cctggatttc gtacttacgc tattccttgc gtttggcgtg acatttgaag
     2761 taccggtcgc cgttgtgatt ctggtccgga tgggtgtagt aagcgtggcc aagctgaagg
     2821 acattcggcc ttacatgatc gtcggtgcct tcgtccttgg tgcggtattc acgccgcccg
     2881 acgtggtatc ccaggtcatg cttgcagtac ccctatggct gctgtatgag gtcggaatct
     2941 acgtatccca gtgggttacc aagccgagta ccgaggagtc cgaaaccagc tcaacgagtt
     3001 aatcgaagaa ggctttgcag aggccgttat tcgagttcgt tacgtctcgc cgggcgcgga
     3061 cgttttccaa cgttcacctt gaccgacatc tcactgcgat ttcgcaaaat cttcaacgta
     3121 gctgcctttc ccggctccag agcagccacc agattcagca tgctggtcga atcaagaaca
     3181 tctctggatt ctaccgccag cagaatatcg ccaggtttga ttcctgcgcg atcagcgggt
     3241 ccaccacgca gcacgccggc aatcaatgtt ccgtgcgcat tggacaattt gaacgattct
     3301 gcgagttccg cagagatatc ctgaacctca actccgatcc agccgcgcgt aactgccccg
     3361 gtccgcacaa tttgttccat cacctgtttc gccaaatttg cagggatcgc gaaaccgatg
     3421 ccaagcgagc cgccgctcct tgagtagatt gcgctattaa cacccaccaa attgccgtat
     3481 gcatcaacaa gcgcaccgcc ggaattgcca ggattgattg ctgcatcggt ctggatgaag
     3541 ttctcg
//
LOCUS       DATMZQ010000197         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4565666, whole genome shotgun sequence.
ACCESSION   DATMZQ010000197 DATMZQ010000000
VERSION     DATMZQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4565666"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..819
                     /locus_tag="VN496_06300"
     CDS             16..819
                     /locus_tag="VN496_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF16595.1"
                     /translation="MVVGSKNFTEQLVLGEIMAQQIENKTHLPVERRFYLAGTYICQQ
                     AILGGRIDIYPEYTGTALTAILKQQPSSNPAEVYNRVKTEYARRFNLALGQPFGFNNT
                     FAIEIRGEDARRLGLKTISQAAAYTPKWRAGFGYEFTGRPDGYKGLAATYGLRFAETP
                     SVMDLGLLARALTNKQIDLAAGNMTDGLIPVLDLFVLEDDRHYFPPYEAVPIIRKQML
                     ARHPEVGQALDELAGKISDDDMRRLNYALDGQHRDVKDVVREFLRLKRQ"
     gene            complement(833..2119)
                     /gene="priA"
                     /locus_tag="VN496_06305"
     CDS             complement(833..2119)
                     /gene="priA"
                     /locus_tag="VN496_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXF16596.1"
                     /translation="MFAPVSDLALVIVDEEQDPSYKQEEMPRYHARDVAVMRAKMANA
                     AVVLGSATPSLESYFNAKKGKYKLLELPDRVEQRPLPEVEIIDMRQEFQETGSEQVIS
                     HRLAEEIKERLERREQVMVLLNRRGYSPVVLCRACGKTLECKNCAIAMTHHKRSRRME
                     CHYCGYTAPVPKACLHCGSEYVYFLGTGSEKLEELLHGMFPQARIGRLDRDTVRGRED
                     FERALNALDEGELDLLVGTQMIAKGHDIHGVTLVGVVGADMALGFPDFRAAERTFQLL
                     TQVEGRAGRGQTPGKVVLQTYFPGHYAVQYAAQHDFAGFYEKELRFRSWMHYPPYSAL
                     ANVLVRSDRLDEALRWSGILGKWFERTRHEGIRVLGPAAAPIMRLKRDYRYHFVVKSA
                     SREKLNSLLRTMLAYAAQQKIPRSQVIVDVDAVWLM"
     gene            complement(2123..>2647)
                     /locus_tag="VN496_06310"
     CDS             complement(2123..>2647)
                     /locus_tag="VN496_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXF16597.1"
                     /translation="NANQQTLIDTLAAAGGKAQVDALQSLDVPRTTLGTLAKRGLVEI
                     VEDPAEFTVSVLKARPSLFDSQFSAAQQVALKVIQQSVNTRKFAGMLLHGVTGSGKTA
                     IYLSAMRAVLEAGRSAILLVPEIGLTPAVAADLHQLFEDEVAILHSALSDKERAEQWR
                     RIKRGEARMVVGTR"
BASE COUNT          563 a          854 c          703 g          527 t
ORIGIN      
        1 ccacgcgccg acgtcatcgt agtcggctcc aaaaacttta ccgagcagct cgtcctcggt
       61 gaaatcatgg cgcagcagat cgagaataag acgcacctgc cggtcgaacg ccgtttctac
      121 ctggccggca catatatttg ccaacaggca atcctcggcg gccgcatcga tatctatccc
      181 gaatacactg gcactgccct caccgcaatt ctgaagcagc aaccgtccag caatcccgcc
      241 gaggtttaca accgggtcaa gaccgaatac gcgcggcggt tcaatctcgc actcggccag
      301 ccttttggat tcaataacac gttcgctatc gagattcgcg gcgaagacgc ccgccgtctc
      361 ggcttgaaaa cgatctcgca agccgccgcc tacactccca aatggcgcgc cggctttggc
      421 tacgaattca cgggacgtcc cgacggctac aagggattgg cggcaaccta tgggcttcgc
      481 tttgccgaga ctcccagcgt gatggatctg ggcctgcttg ctcgcgcgct cacgaacaaa
      541 caaatcgatc tggctgcagg aaacatgacc gatggcctga tcccggtgct ggatctgttc
      601 gtcctcgaag acgaccgcca ctacttcccg ccgtatgagg cggtccccat cattcggaaa
      661 cagatgctgg ctcgccaccc tgaagtggga caagcgctgg acgaactggc aggaaagatt
      721 tcagacgacg acatgcgccg gctgaactat gcgttagatg gccagcatcg tgacgtgaag
      781 gacgtagtaa gagagttcct gcgcctgaaa aggcaatgaa caccagggaa agttacatca
      841 accagaccgc gtccacgtcg acaatcacct gcgagcgcgg aatcttctgt tgggcggcgt
      901 acgccagcat tgtcctgagc aacgaattca gcttctctcg gctggcggac ttcaccacga
      961 agtgatagcg gtagtctcgt ttcaagcgca tgatcggcgc ggccgccgga ccgagcacgc
     1021 gaatcccttc atgccgggtt ctctcaaacc actttcccaa gatcccggac cagcgcagcg
     1081 cctcatccag tctgtcgctc cgcaccagca cgttggccaa tgcgctgtaa ggcggataat
     1141 gcatccagct gcgaaagcgc agctcttttt catagaagcc cgcaaaatca tgctgcgccg
     1201 cgtactgcac agcatagtga ccgggaaaat atgtttgcag cacgacttta cccggagtct
     1261 gtccgcgacc cgcgcgcccc tcaacctgag tcagcaactg gaaggtacgc tccgctgctc
     1321 gaaagtcagg aaacccgagc gccatgtcgg cgccgacaac gcctaccagc gtgaccccgt
     1381 gaatgtcgtg gcccttggcg atcatctgcg tgcccaccag cagatcgagt tcgccttcat
     1441 cgagagcgtt cagggcccgt tcgaagtctt cgcggccgcg caccgtatcg cggtccagac
     1501 gtcctatgcg tgcctgcgga aacattccat gcagcaattc ttccagtttc tccgacccgg
     1561 tgccgaggaa ataaacatac tcgctgccgc aatgaaggca agccttgggc acgggcgcag
     1621 tgtacccgca atagtggcat tccatgcggc gagagcgctt gtgatgagtc atggcgatgg
     1681 cgcagttctt gcactccagc gtcttgccgc acgcccggca caacactacc ggcgaatatc
     1741 cccgccggtt cagcaacacc atgacttgct ccctccgttc cagccgttct ttaatctctt
     1801 ccgccagcct gtgcgagatg acctgctcgc tcccggtttc ctgaaattcc tggcgcatgt
     1861 cgatgatttc gacttccggc aggggacgtt gctccacccg gtcgggcaat tcgagcaact
     1921 tgtatttccc tttcttggca ttgaagtacg attccagcga cggtgtggcc gatcccagca
     1981 ccacggccgc attggccatc ttggcccgca tcacagctac atcgcgggcg tgatagcgcg
     2041 gcatctcttc ctgcttgtac gagggatcct gctcctcatc cacgatcacc aacgccagat
     2101 cgctgacagg agcgaacacc gcctaacgcg tccccaccac catgcgcgcc tcgccacgct
     2161 tgatgcgacg ccattgttcg gcccgttcct tgtcagaaag cgccgagtgc aggattgcaa
     2221 cttcatcttc gaacaattga tgcaggtcgg cagccaccgc cggcgtcagg ccaatttcag
     2281 gaaccagcaa aatagccgaa cgcccggcct caagcaccgc ccgcatcgcc gaaagataga
     2341 tcgcggtctt tcccgatcca gtcacgccat gcagcagcat gccggcgaac ttgcgggtgt
     2401 tcacactttg ctgaataacc ttgagcgcca cctgttgtgc cgcgctgaac tgtgagtcaa
     2461 acaggctggg acgggccttc aataccgaca ccgtgaattc ggccggatct tcgacaattt
     2521 ccactaaccc gcgcttcgcc aatgtaccca aagttgtgcg cgggacgtct aacgactgca
     2581 gggcatcgac ctgcgctttg ccacccgcag cggcgagagt gtcgatgaga gtttgctggt
     2641 tggcgtt
//
LOCUS       DATMZQ010000198         4654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3260124, whole genome shotgun sequence.
ACCESSION   DATMZQ010000198 DATMZQ010000000
VERSION     DATMZQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3260124"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VN496_06315"
     CDS             complement(<1..690)
                     /locus_tag="VN496_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXF16598.1"
                     /translation="MAPHAELLAVLLLGVVAGTLGGVIGFGSSIMLMPVLVMLYGPRE
                     AVPIMAVAAVLANASRVLVWWREVQWRAVAAYAATGVPAAALGASTLLIIPQQWVEFV
                     MGCFFLAVIPGRRWLARHNLRVTLWGLAWCGAVIGYLTGIVVSTGPINAPVFLAFGLV
                     KGAFIGSEALGSLALYISKAVVFRSFGALPADIVIKGMIVGSSLMAGSFAARWLVLKL
                     AAEQFRLLIEAL"
     gene            815..1765
                     /locus_tag="VN496_06320"
     CDS             815..1765
                     /locus_tag="VN496_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXF16599.1"
                     /translation="MELRQLEYFVAIAETGAFSRAALRLSVGQPILSRQIKALELELG
                     TELYHRTGRGIVLSEAGKILEQHARGVLETTAAARRAIQALGSGPAGRVVIGMPPSVG
                     AVLTASLVRQFREQFPHVSLGVMEGFSGHVLEWLTTGRIDVAVLYNAPRTSALMSEPL
                     LTDELFLLGPVADPAGAGTGQVAGIALANIPLILPSRPHGLRMLVDELLAGIGVTPNV
                     QVEIDAMPSTLSLVEAGIGYTILSYSSAHHLLEARRIRKWSIVDPAMTRSLVVATSMQ
                     RPVTKAARALVGFVRKQVDALVAEGRWTPRQSRSVQEIRT"
     gene            1762..2745
                     /locus_tag="VN496_06325"
     CDS             1762..2745
                     /locus_tag="VN496_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXF16600.1"
                     /translation="MRTFAMFTRGFAFALVLLLAAGMARAADAYPNRPIRWVVPWPAG
                     GIADTQARIIADQLGKALGQPVVIDNRAGASGTLGADLVAGAKPDGYTLLYISPNEQA
                     IAQAIGMKVRYDAQKDFTPITQMLRRPAVLVVNASLNVNSVAELVTLAKTDRNLSYGT
                     PSVAHFCHFATEIFNRRAGIKVTAVPYKGEAPMINDLIGGQVAYGFGFATTVAPTVAA
                     GRLRALATTGAARSPQLPDVPTFRELGMPEVQMYIWTGIAGPAGLPGEITARLQSALN
                     KILNSPAIKSRREFADSEIVASKPDEFRVFLAAERVRWTKLVAETGITVDN"
     gene            complement(2779..3576)
                     /locus_tag="VN496_06330"
     CDS             complement(2779..3576)
                     /locus_tag="VN496_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXF16601.1"
                     /translation="MEQDVYEIHAIKYARLMRRSPENFIGGDVHDTEMPLNYYVWVIS
                     NTQRRIVVDTGFSEAMAKKRNRQIVRPVAEGVTALGLEPGKVSDVIITHMHYDHAGNI
                     PLFANARYHLQDQEMEFATGRCMCHGMMRHGYEPDDISHLVRCVFDQRVQFHDGAGEV
                     APGIELHHIGGHTKGMQAVRVRTRRGWVVLASDASHFYAHMEQDRAFPILYNLGDMLE
                     GYNTLRRLATSREHIIPGHDPLVMERYPAARTGMEDWIVRLDADPKK"
     gene            complement(3608..3997)
                     /locus_tag="VN496_06335"
     CDS             complement(3608..3997)
                     /locus_tag="VN496_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXF16602.1"
                     /translation="MNDDLFKKGLEIRKSVLGAEFVEKSLAAADDFNMPMQRLTTEYC
                     WGEIWGRQELPKKTRSMLNLAMLCALNRPHELKMHIAGALRNGVTRVEIREVLLQVAI
                     YCGIPAGVDAFRVAKETFAEFDRTEKK"
     gene            complement(4026..>4654)
                     /locus_tag="VN496_06340"
     CDS             complement(4026..>4654)
                     /locus_tag="VN496_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF16603.1"
                     /translation="KLGLKLVVDETFTPPLANATPLIQKVRSARPDILILLPTVISDA
                     KLLLEKMNEFGLGRGKVPTIANGAAILDPDLRNNMNIEQLEGVMAVVADWTTSAQKDF
                     VAEVKKKTGEPWPTQHFVTTYGDILIFKAALEAAGKADREAVAKALHAMNLSGGPADF
                     FPGNGKLKFDDNGRREGASLLIVQWQKGVPITVYPAKDAIAAPIWPKQ"
BASE COUNT          884 a         1461 c         1399 g          910 t
ORIGIN      
        1 caatgcttcg attagcaggc ggaactgttc ggccgcaagc ttgagcacca gccacctcgc
       61 ggcgaaggag cccgccatca gcgagctgcc gacaatcatg cccttgatga cgatgtccgc
      121 gggcagcgcg ccaaaactgc gaaacacgac cgccttgctg atatacaacg ccagtgagcc
      181 cagtgcctcg ctgccgataa acgcgccctt gaccaatccg aaggccagaa ataccggcgc
      241 attgatggga ccggtggaga ccacgatgcc ggtgagatag ccgatcaccg ctccacacca
      301 tgcaaggccc cacagcgtga cgcgcaagtt gtgcctggcg agccagcgtc gaccggggat
      361 caccgcaaga aaaaaacaac ccatgacgaa ctcgacccac tgttgcggaa tgatcagcag
      421 cgtcgaggcc cccaaagctg ctgccggcac gccggtggcc gcatacgccg cgactgcgcg
      481 ccactgcacc tcccgccacc agaccagcac gcgcgatgca ttggccagca cggcagccac
      541 cgccatgatc ggcaccgctt cacgcggccc gtacagcatc accagcaccg gcatcagcat
      601 gatggatgag ccgaagccaa tgacgccacc cagcgtgcca gccaccacgc ccagcagcaa
      661 taccgcgagc aattccgcat gcggggccat gctatgcgtc cagccgggcc gaggtgcatc
      721 tgtggggtaa aatgaaacga tcctttcgtg caacgatccc gcaagcggta tacggcttgc
      781 gccgcaaccc gatgtcattg accggccact tgcaatggaa ctgcggcaac tcgaatattt
      841 cgtcgccatc gctgaaaccg gcgcgttctc gcgcgcagcg ctgcgactgt cggtcggaca
      901 acccatcctg agccggcaga tcaaggcgct ggagctggaa ttgggcactg aactttatca
      961 ccgcacgggg cgcggcatcg tgctgagcga ggccggcaaa attctcgaac agcatgcgcg
     1021 cggcgtgctg gagaccactg cagcagcgcg tcgcgccatc caggcgcttg gcagcggacc
     1081 cgcgggccgc gttgtgatcg gcatgccgcc ctccgtgggc gcagttctga ccgcgtcgct
     1141 ggtgcgccag ttccgcgagc aatttccgca cgtttcgctg ggcgtcatgg aaggcttcag
     1201 cgggcatgtg ctcgaatggc tcacgaccgg ccgtatcgac gtggcggtgc tgtacaacgc
     1261 accgcgcacc agcgcgctca tgagtgaacc gctgctcacc gacgagttgt ttctgctggg
     1321 tccggtagca gatccggccg gcgcaggcac tgggcaggtt gccggtatcg cgctggcaaa
     1381 cattccgctg attctgccga gccgtccgca tggactgcgc atgctggtcg atgagctgct
     1441 cgccggcatc ggcgtcacgc ccaacgtgca agttgaaatt gacgcaatgc cgtccacgct
     1501 gagcctggtc gaagccggca tcggttatac gattctgtcg tattccagcg cgcatcatct
     1561 gcttgaagcc aggcgcatcc gcaagtggtc catcgtggat ccagccatga ccagatcgct
     1621 ggtcgtggca acctccatgc agcgcccggt gaccaaggct gccagggcgt tggtcggatt
     1681 cgtgcgcaag caagtcgatg cgctggtggc cgaaggccgc tggactccgc ggcaaagcag
     1741 atcagtacag gagatccgga cttgagaacg tttgcgatgt tcactcgcgg ctttgcattc
     1801 gcgctggtac tgctgctcgc tgccggtatg gctcgggcag cggacgcgta tcccaatcgc
     1861 cccattcgct gggtcgtgcc ctggccggcg ggcgggatag cggatacgca ggcgcgcatc
     1921 attgccgatc agctgggcaa agcgctcggg cagccggtcg ttatcgacaa ccgtgcaggc
     1981 gcgagcggca cgctgggtgc cgatttggtc gcgggcgcaa agccggacgg ctatacgctg
     2041 ctgtacatat cccccaacga gcaagcgatt gcgcaggcca tcggcatgaa ggtgcgctat
     2101 gacgcgcaaa aagacttcac gccgatcacg cagatgctgc gccggccggc ggtgctggtt
     2161 gtcaatgcgt ctctcaacgt gaacagcgtt gctgaactgg tcacgctcgc caagacggat
     2221 cgaaatttaa gctatggaac gccaagcgtc gcgcacttct gccactttgc gacggaaata
     2281 ttcaatcgca gggccggcat caaggtaacc gccgtgccgt ataaaggtga agcaccgatg
     2341 atcaacgatt tgatcggcgg gcaagtggcg tacggctttg gctttgcgac caccgtggcc
     2401 ccgactgtag cggctggacg cctgcgcgcg ctcgcgacca cgggagcggc gcgctcgcca
     2461 cagttgcccg atgtgccgac attccgcgaa ctcggcatgc cggaagtcca gatgtatatc
     2521 tggaccggaa tcgcgggtcc cgctggcttg cccggcgaaa tcacagcccg cctgcaaagc
     2581 gcgctcaata aaatattgaa cagtcccgcg atcaagtcgc gccgggaatt cgctgattct
     2641 gaaatcgtcg ccagcaagcc cgatgagttt cgggtgtttc tcgccgccga gcgcgtgcgc
     2701 tggacaaagc tggttgcgga aaccggaatc accgtggaca actagatgct caacgcgtcc
     2761 gccagcagac gggcgcagct acttcttcgg atcggcgtcc agccgcacga tccagtcttc
     2821 catgccggtt cgcgcggccg ggtaacgctc cattaccagc ggatcgtgcc cgggaatgat
     2881 gtgctcgcgc gaagtcgcca gccggcgcag cgtgttgtag ccctccagca tgtcgcccag
     2941 gttatacaga atcggaaatg cacgatcctg ctccatgtgc gcatagaagt ggcttgcatc
     3001 cgatgcgagc actacccaac ctctgcgtgt gcgcacccgt accgcctgca tgcccttggt
     3061 atgcccgccg atatgatgca gctcgatgcc tggcgcaact tcgccggcac cgtcatggaa
     3121 ttgcacgcgc tggtcgaaca cgcagcgcac cagatgtgaa atgtcgtccg gctcgtaacc
     3181 atgccgcatc atgccgtggc acatgcagcg gccggtggcg aattccattt cctggtcttg
     3241 caggtgatag cgggcattgg cgaacagcgg gatgttgccg gcatggtcgt aatgcatgtg
     3301 ggtgatgata acgtcgctga ccttgccggg ctcgagtccg agcgcagtga cgccttcggc
     3361 gaccggccgc acgatctgcc ggttgcgctt ctttgccatg gcctcgctga aaccggtgtc
     3421 gaccacgatc ctgcgttgcg tgttcgagat gacccacacg taataattca atggcatctc
     3481 ggtgtcgtgc acgtcgccgc cgatgaagtt ttccggcgaa cggcgcatga gccgcgcata
     3541 tttgatcgca tggatctcgt agacatcttg ttccattgcc gtgctctcct tgtggttcga
     3601 agccggctta tttcttctcg gtccgatcga actctgcgaa tgtttccttt gctacccgga
     3661 aggcgtccac gccagccggg atgccgcaat agatggccac ctgcaatagc acttcacgga
     3721 tttccacccg cgtcacgccg ttacgcagcg cgccggcgat gtgcatcttc agctcgtgcg
     3781 gccggttcag cgcgcacagc atggccaggt tcagcatgct gcgtgttttt ttcggcaatt
     3841 cctggcgtcc ccatatctcg ccccagcagt attccgtggt gagcctctgc atcggcatgt
     3901 tgaaatcgtc cgctgccgcc agtgattttt ccacgaactc ggcgcccagc accgacttgc
     3961 ggatttccag gcccttcttg aacagatcgt cattcacgtt gtttctcctg ttgggtggat
     4021 agtcgtcatt gcttgggcca gatgggcgcg gcgatcgcgt ccttggctgg atacacggtg
     4081 atcggcacac ctttctgcca ttgcacgatc aaaaggctgg cgccttcgcg ccggccatta
     4141 tcgtcgaact tgagcttgcc attgccgggg aagaaatcgg cgggaccgcc gctgaggttc
     4201 attgcatgca acgccttggc aaccgcttcg cgatcggctt ttcccgcagc ctcgagtgcg
     4261 gccttgaaga tcaggatatc gccataggtc gtaacgaaat gctgcgtggg ccacggttcc
     4321 ccggtcttct tcttcacttc cgcaacgaaa tccttttgtg cgctggtggt ccagtcggca
     4381 acgaccgcca tgacgccctc gagctgctcg atattcatgt tgttgcgcag atcgggatcg
     4441 agaatggcgg caccgttggc gatggtgggc actttgccgc gcccgaggcc gaattcgttc
     4501 attttctcga gcaacagctt tgcatccgag atcaccgtgg gaagcaggat caggatatcg
     4561 ggccgcgccg aacgcacttt ctggatcagc ggcgtcgcat tcgcgagcgg cggcgtgaac
     4621 gtttcgtcga cgaccagctt caggcccagt tttt
//
LOCUS       DATMZQ010000199        10181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1881684, whole genome shotgun sequence.
ACCESSION   DATMZQ010000199 DATMZQ010000000
VERSION     DATMZQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881684"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VN496_06345"
     CDS             complement(<1..1454)
                     /locus_tag="VN496_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF16604.1"
                     /translation="MSTYAAPIRDMKFAVKELIGVDYFAGLPGCEDLTPDVVDAVLEE
                     AGKFATGVLDPLNRPGDITGAKLLANGVASPPGFSEAFKQFSAGGWAGLNCDPEYGGQ
                     GLPHIISAQTSEMWNSANMSFCLCPMLTAGVVASLMRHGSSVQRATYLPNLVSGKWTG
                     TMNLTEPQAGSDLSAVRARAVPEGDHFRIHGTKIFITWGEHDMAENIIHMVLARTPQA
                     PEGVKGISLFVVPKFLVESDGGIGKRNDVKCVSIEHKLGIHGSPTCVMAYGEAEGAVG
                     YLVGEENRGLEYMFTMMNFARLEVGIEGVAIAERAYQHALEYAKSRVQGREIGVKSGD
                     RVSIIHHPDVRRMLMSMKAQTEAMRSLATLASAYLDQALLHPDKTERAKNQAMFDLLT
                     PVVKGWCTEQSIEIASTGVQVHGGMGFVEETGAAQYLRDARITTIYEGTTGIQANDLV
                     GRKIAYEKGSTINLLLAEMRAVDARLAKVGHPGLA"
     gene            complement(1526..2455)
                     /locus_tag="VN496_06350"
     CDS             complement(1526..2455)
                     /locus_tag="VN496_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXF16605.1"
                     /translation="MAILIIGEHDNAAIKPAVLNTVAAAQEIGGDIDILIAGYQCATA
                     AQGAAGIPGIRKILMADAPHYAHALAENISALVVSIAKNYSHVLAPSTSFGKNVLPRV
                     AALLDVAQISDIIKVESTDTFVRPIYAGNALATVQASDAIKVITVRSTGFDPVPIAGG
                     NAAIESIAAQPDSGHSSMMGQELTKSARPELTAARAIVSGGRGMGNGENFKILEALAD
                     KLGAAVGASRAAVDSGFVPNDYQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDAEAPIFQVADYGLVADLFQAVPELTAQL"
     gene            complement(2455..3204)
                     /locus_tag="VN496_06355"
     CDS             complement(2455..3204)
                     /locus_tag="VN496_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXF16606.1"
                     /translation="MKVLVPVKRVVDYNVKVRIKPDGSGVETANVKMSMNPFDEIAME
                     EAMRLKEAGIATEIVAVSIGGAQCQETLRTALAIGADRAILVETSVEVQPLGVAKLLK
                     AIVAKESPIMVIMGKQAIDDDSNQTGQMLAALLNWSQGTFASKIHVKGKTAEVTREVD
                     GGLETVLLKLPAVVTTDLRLNEPRYVTLPNIMKAKKKPMETLTPEAVGVDVSPRLKVL
                     KVQEPPKRTAGKVVKSVPELVEKLRSEAKVI"
     gene            complement(3339..4970)
                     /locus_tag="VN496_06360"
     CDS             complement(3339..4970)
                     /locus_tag="VN496_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HXF16607.1"
                     /translation="MNRDSPYQTGLDRNPANYVPLSPLSFLPRAAEVYPSRTAIVHGD
                     RTATWSQTYQRCRRLASALVGHGIGVGDTVAIMAPNVPAMFEAHFGVPMSGAVLNTLN
                     TRLDPDAIGFMLNHGEAKVLFTDREFSSTISAALRHVKRKILVVDIDDALGQEGERLG
                     ESDYEAFLATGDADFLWSLPDDEWDPIALSYTSGTTGDPKGVVTHHRGAYLNSVGNIV
                     TWSMPHFPVYLWTLPMFHCNGWCFPWTLAALAGTSVCLRRVDAESVFNLMRKHKVTHY
                     CGAPVVHNMLINAPTEMREGIPKKIFGMIAGSAPPSAVIENLERMGVDLTHVYGLTEV
                     YGPASVCAKHPEWDALSPSRRAELNARQGVRYEMQESMTVLDPKTMQPVPRDGQTMGE
                     IFFRGNIVMKGYLKNQRATSEAFAAGWFHTGDLAVVDAEGYVKIKDRSKDIIISGGEN
                     ISTIEVEDVLYSHPHVLEAAVVARPDEKWGETPCAFVVLKAGSPLVTEKQLIAFCRER
                     LAHFKCPRKVIFGSLPKTSTGKIQKNVLRDLARKA"
     gene            5251..6489
                     /locus_tag="VN496_06365"
     CDS             5251..6489
                     /locus_tag="VN496_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16608.1"
                     /translation="MMPERANRTFICSVLFLDIAEYSKKPVSDQIQLKDRFNALIAES
                     IRDIAPSDRIILDTGDGVAVNFLGDPEDALFVAMSLREAFAPQTADLRHLPARIGINL
                     GPVRLVRDLNGQPNIIGDGINVAQRIMGFAQPGQILVSRSYYEVVLHISEGYTKLFNY
                     EGSRTDKHVREHELYSVGYTSSRSSGIRSPAERAKVSTHDNKQQATTATRVTGPATVS
                     IIARMEPWFTNRTIVFGTATLSAGAFLLSVVVNLLGQGEVAALPSQTAERSVQKKISP
                     PRLEETHAATVQKPVEKRIKTAAAHNEAALPQPKPETYEKKEAKFSPFFFFLKHKAPA
                     EPDPVPAPVGSAMVMLAIAPWGEIYVDGDRVGVSPPVNEVEVAPGKRTIEIRNGTFPV
                     FTQVLDVRADQKIRIKHKFD"
     gene            6525..6890
                     /locus_tag="VN496_06370"
     CDS             6525..6890
                     /locus_tag="VN496_06370"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HXF16609.1"
                     /translation="MTLLLAGCQTDGLFKGQGATRHPGDSFLQEGIEDYEEGNYRTAK
                     RRLQFALEEGLSRPDRVKAHKYLAFIACVSNQQLACREEFAVALELDPAFELSAAEAG
                     HPIWGPVFKNIKAKKTKQP"
     gene            6887..8230
                     /locus_tag="VN496_06375"
     CDS             6887..8230
                     /locus_tag="VN496_06375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXF16610.1"
                     /translation="MNGEPTISTLGRYEILSELGQGSMGVVYRARDPMLDRIVAIKTI
                     NLTLPKDELAEYEARFYQEAKAAGGLSHRNIVTIHDIGRSERVAYMAMEFLEGQELRR
                     LLQARTPIGMAHALDVGAQVAEGLQYAHEGHIIHRDIKPANIMVLNDGLVKITDFGIA
                     RMRTNEVKTMTGMILGSPKYMSPEQVSGKRADTRSDIFSLGVVLYEMLTGASPFVADN
                     IHGVMYQTMNFNPPAPKTLNPELPEVLNFIIAKALAKNLDDRYQKAAELSNDLRRVIS
                     ALTGDSGVPQDVVSGPLPAPFIEPISPLTRQEDKQARILGMADDADLHHAAEVPSQPE
                     QLPSAESSVPALGLSRAFDSFDATMRLAALTGVEKQLDTFSETQKIARLKALADAKRQ
                     AASAAASTLSGSATWKQLATERPNPASPMPVNQVKLIWLGSGALAIAAALLFLLR"
     gene            complement(8237..9589)
                     /gene="radA"
                     /locus_tag="VN496_06380"
     CDS             complement(8237..9589)
                     /gene="radA"
                     /locus_tag="VN496_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXF16611.1"
                     /translation="MAKPKTIYSCTECGGQTLKWQGQCPHCTAWNTLVESIAEKAGGS
                     RYQSVAKSSKLQKIADVDAREEPRRPTGIDELDRVLGGGLVPGAVVLIGGDPGIGKST
                     LLLQALSILGNSCNAIYVSGEESVQQIALRARRLALNAASLNLMAEIELEKVMSMLAT
                     QKPDVAVVDSIQTVYSEALQSAPGSVAQVRECAAQLTRFAKSSGTALLLVGHVTKEGA
                     LAGPRVLEHMVDAVLYFEGDTHSSFRLIRAIKNRFGAVNELGVFAMTERGLKGISNPS
                     ALFLSQHGTQVAGSCVMVTQEGSRPLLVEVQALVDEAHAPNPRRLSVGLEPNRLAMLL
                     AVLHRHAGIAAFDQDVFVNAVGGVKIDEPAADLPVLLAIVSSLRNKALPEKMVAFGEI
                     GLAGEVRPVQRGQERLKEAAKLGFKLAIIPKANMPRANIDGIEVVSVERVEEAVARVR
                     "
     gene            complement(9595..>10181)
                     /gene="alr"
                     /locus_tag="VN496_06385"
     CDS             complement(9595..>10181)
                     /gene="alr"
                     /locus_tag="VN496_06385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXF16612.1"
                     /translation="AEGPGGIQKQMREFDSCVARPNYERSLANSAALLRFPEARGNWV
                     RPGLMLYGASPLHDISAEKLGLKPVMTLKSEVIAIQKLKPGDTVGYGGVFTADHDMRI
                     GVIACGYADGYPRQAANGVSVLIEGTRVPIAGRVSMDMLCVDLTHVPHARVGSPAVLW
                     GEGLPIEEVAGHAGTVSYELMCALAARVPVREKV"
BASE COUNT         2212 a         2958 c         2737 g         2274 t
ORIGIN      
        1 gccaggccag ggtgaccgac tttagcgagc cttgcgtcga cagcacgcat ctcagccaac
       61 aggagattta tggttgagcc cttctcgtaa gcgatcttgc ggccgaccaa gtcgttcgct
      121 tgaattcctg tggtgccttc gtagatcgtc gtgatgcgtg catcgcgtaa atattgtgca
      181 gcgcccgtct cctccacgaa tcccatcccg ccatgtacct gcactcccgt cgatgcaatt
      241 tcaatcgact gctcggtaca ccaacctttg accactggcg tgagcaggtc aaacatcgcc
      301 tggtttttcg cgcgttcagt cttatctgga tgcaacagtg cctgatcaag ataggccgaa
      361 gccagtgtgg caagcgatcg cattgcctct gtctgcgctt tcatgctcat cagcatgcga
      421 cgcacatccg ggtggtgaat aatactgacg cgatcaccag atttaacccc gatttcccgg
      481 ccctggacgc ggcttttagc gtattcgagc gcatgctgat aagcacgttc agcgattgcc
      541 acgccttcta tgccgacttc gagccgtgcg aagttcatca tcgtgaacat atattcgaga
      601 ccgcgatttt cctcaccgac cagatatccg accgcgcctt cggcttctcc ataagccatg
      661 acgcaagtgg ggctgccatg tatgccgagc ttgtgctcaa tggagacaca tttgacatcg
      721 ttgcgctttc cgatgccgcc atccgattcg accaggaact tagggacgac aaaaagcgaa
      781 atgcctttga caccttccgg cgcctgcggt gtgcgagcga gcaccatatg gatgatgttt
      841 tcagccatgt cgtgctcacc ccaggtaata aatatctttg tgccgtggat gcggaaatga
      901 tcgccttccg gcacggctcg cgcccgcacc gccgaaaggt cggagcccgc ctgcggctct
      961 gtcaaattca tggtccctgt ccattttccg cttaccaggt tcggcagata ggttgcccgc
     1021 tgaacagatg atccatggcg catcagggag gcgactacac ctgcggtgag cattgggcac
     1081 aaacaaaaag acatattcgc cgagttccac atctcggatg tttgagcgga aataatgtgc
     1141 ggcagcccct gtccgccata ttctgggtcg caattcagcc cggcccagcc tcccgccgaa
     1201 aactgcttga aagcttcgct gaatccgggc ggtgaagcga ccccgttggc gagcagcttg
     1261 gcgccggtga tatctcccgg ccggttcagc gggtcgagaa cgccagtcgc gaatttgccg
     1321 gcctcttcca gtaccgcatc gacgacatca ggtgtgagat cttcacaccc gggcagaccg
     1381 gcaaagtaat cgacaccgat caattccttt accgcgaatt tcatgtctcg aatgggtgct
     1441 gcgtaagtag acatcgttgc tcctgacaac aaactctatt gactcaccct gaacgcgcct
     1501 cgggcgggtg caaggaaagc aagggtcata actgcgccgt cagctccggc accgcctgaa
     1561 acaaatcggc gaccaagcca taatcggcta cctggaaaat cggcgcctcg gcatccttgt
     1621 tgattgctac gataaccttg ctgtccttca tacccgccaa atgttgaatc gcgccggaga
     1681 tacctactgc gatgtacagc tctggcgcca ctattttgcc agtctgtccg acctggtaat
     1741 cgtttggcac gaatccagaa tcgactgctg cgcgagaggc gcccaccgcg gcgccaagtt
     1801 tgtcggcaag cgcttccagg atcttgaaat tttccccatt ccccattccg cgaccgcccg
     1861 atacgatcgc ccgcgccgcc gtgagttccg gacgcgcgga cttggtcagt tcctgaccca
     1921 tcattgatga atggccgctg tctggttgcg cagcgatact ttcgatagca gcattgcctc
     1981 cagcgattgg cacgggatca aagccggttg accgtacagt gatgaccttg atggcgtccg
     2041 aagcttgcac cgttgccaac gcattgccgg cataaattgg acggacgaag gtatcggtcg
     2101 attcaacctt gataatgtcc gaaatctggg ccacatcgag cagagcagct acgcgcggca
     2161 ggacattctt gccaaagctt gtggatgggg ccagaacatg gctgtagttc ttggcaatgg
     2221 aaacgaccag cgccgaaatg ttctccgcca gtgcatgggc atagtgtggc gcgtcggcca
     2281 tcagtatttt cctgattccc ggaattcctg cggcaccttg ggctgcagtc gcacactggt
     2341 atcccgcaat cagtatatcg atgtcgccgc cgatttcctg tgcggctgca actgtgttga
     2401 gtaccgccgg tttgatcgcg gcattgtcat gttccccaat gatcaggatg gccatcagat
     2461 gactttcgct tcgcttctga gcttttcaac caactccggt acgcttttga ctaccttgcc
     2521 tgcggttcgt tttggtggct cctgaacctt gagcaccttg agacgcgggg atacatccac
     2581 gccgactgct tcgggggtca aggtctccat gggtttcttt ttcgccttca tgatatttgg
     2641 tagtgtcacg tagcgcggtt cattcagtct aagatcggtg gtcacaactg ccggcagttt
     2701 cagcagaaca gtttcgagtc cgccatcgac ttcgcgggtg acttcagcgg ttttaccttt
     2761 gacgtgtatc tttgatgcga aggttccctg agaccaattc aatagcgctg caagcatctg
     2821 cccggtttga ttggaatcat catcaattgc ctgcttgccc ataatcacca taatcggtga
     2881 ttccttcgcc acgattgctt taagcagttt ggcaaccccc agcggctgga cttcgacgct
     2941 ggtctccaca aggattgcac ggtctgcgcc gatcgcgagc gccgtgcgca aagtctcttg
     3001 acattgagcg ccaccgatgg agaccgcgac aatttccgtc gcgataccag cctccttcaa
     3061 acgcatggct tcctccattg cgatctcatc aaacggattc atcgacattt tgacattggc
     3121 ggtttccacg ccagatccgt ccggcttgat gcgcaccttg acgttgtagt ccaccacccg
     3181 cttgacgggc accagtactt tcatctcaaa aatccttgcc gtgggaaata aaccagattc
     3241 tacagcagca ccttcgaggc ctgtcgtgcg tgccatcacg caaagctaat tgagatggcg
     3301 ttgccgcttg cacagtcatg cgtggcaaaa gttgcctgtt acgcttttct cgcgagatcg
     3361 cgcagcacgt ttttttggat ctttccggtc gaagtcttgg gcaacgaacc aaagataact
     3421 ttgcgcgggc acttgaaatg agccagtcgt tcccggcaga aagcgatgag ctgcttttca
     3481 gtcacgagcg gcgaaccggc ttttagtact acgaatgcgc agggcgtttc accccacttt
     3541 tcgtccggac gggctacgac ggccgcctcc agaacatggg ggtgactgta gagcacgtct
     3601 tcaacctcaa tagtcgatat attttcgcca cccgagatga tgatgtcctt ggagcgatct
     3661 tttatcttga cgtacccttc tgcgtcgacc actgcgagat cgccggtatg gaaccagcca
     3721 gccgcaaaag cctccgaggt ggcgcgctga ttctttagat atcctttcat cacgatgttc
     3781 ccacgaaaga aaatttcccc catggtttgg ccgtcacgtg gcactggttg catcgtcttc
     3841 gggtcgagca cagtcatgct ttcctgcatt tcgtagcgaa cgccctgccg ggcattcagt
     3901 tcggcacgtc tcgatggcga caaagcatcc cattctggat gtttcgcgca aaccgaagct
     3961 ggaccgtaca cttccgtcag gccgtaaaca tgggtcaggt cgacgcccat gcgttccagg
     4021 ttttcgatga ccgccgatgg gggcgcagag cccgcaatca ttccaaagat ttttttgggg
     4081 atgccttccc tcatttccgt cggcgcgttg atgagcatgt tgtgcaccac gggtgctcca
     4141 cagtaatgag tcaccttatg tttacgcatg aggttgaaca ctgattccgc gtccacgcga
     4201 cgcaggcaga cactggtccc cgcaagagcg gccagcgtcc aaggaaagca ccaaccattg
     4261 cagtgaaaca ttggcaaagt ccacaaatac acggggaagt ggggcattga ccaagtgacg
     4321 atgttaccga cggagtttag atacgctcct cgatgatgcg taaccacacc cttgggatcg
     4381 cctgtcgtgc ccgaggtgta cgacaatgcg atggggtccc attcatcgtc cggcagggac
     4441 cacaaaaaat ccgcatcacc ggtcgcgaga aaagcctcgt aatcgctttc gcctagacgt
     4501 tccccttcct gccccagcgc atcatcaatg tcgacaacca gtatttttcg cttcacatgt
     4561 cgcaacgcag cagaaatggt ggaggagaat tcccggtctg tgaaaagaac ctttgcctcc
     4621 ccatggttca gcatgaatcc aatggcgtca ggatcaagtc gcgtatttag cgtattgagc
     4681 acagcgccgc tcatgggaac gccaaagtgc gcttcaaaca tcgcggggac attgggagcc
     4741 atgattgcca ccgtgtcgcc gacgccaatg ccgtgcccga ccagggctga tgccagcctg
     4801 cgacaacgct gataagtctg gctccaggtc gctgttctgt ctccgtgcac gatggcagtt
     4861 ctgctcggat aaacctccgc cgctcgcggt agaaaagata atggggatag tggtacgtag
     4921 ttcgcggggt ttcgatccaa ccccgtttgg tatgggctgt cgcggttcaa gcgttgctcc
     4981 aatcaagtaa accaacccgg ctcggcaaag gcgttgctag catgccgggc cgcttgggaa
     5041 tcatacggga ttaccttagc gaagcccggc gattgactta gccggtctac ctagaattcc
     5101 gtgtaagagt gaccccaaag ggtttgacaa tataagaaaa tctgccttct ggttctagcg
     5161 aaaaccgact gattcatgta gcatctacca cttgggggcc ttgttccaat caagggatgg
     5221 tttgatgccg acgcagcagg tgacgctgag atgatgccag agcgcgccaa ccgcacgttc
     5281 atctgcagcg tgttgttcct cgacatcgct gagtactcca agaaaccagt ctccgatcag
     5341 attcagctca aggatcgatt caacgcgttg attgcggaga gcatccgcga catcgctcca
     5401 agcgaccgca tcattctgga tacgggggat ggcgttgccg taaattttct cggcgacccc
     5461 gaggatgcgc tcttcgttgc catgagctta cgcgaggcgt ttgcccctca gactgcggac
     5521 cttcggcacc tgcccgcacg cattggcatc aacctgggac cggtgcgcct ggttcgcgat
     5581 ctcaatgggc agccgaacat catcggcgat ggtatcaatg tcgcccagcg gataatggga
     5641 tttgcgcagc cgggacaaat ccttgtgtcg cggtcttatt acgaagtcgt gctgcacatt
     5701 tcggagggct ataccaagct gttcaactac gaaggatccc ggactgacaa acacgtgcgg
     5761 gagcacgaac tctacagcgt ggggtacacg agttcgcgtt cgagcggcat ccgctctccc
     5821 gcggagcgcg caaaggtctc tacacacgac aacaaacaac aagctacaac cgccacgcgc
     5881 gtcaccggtc cagccaccgt ttcaatcatt gccaggatgg aaccttggtt taccaaccgc
     5941 acgattgtgt tcggaaccgc gacgctgtca gcaggcgcat tcctgctttc ggtggtagtg
     6001 aacctgctgg gccaaggcga ggtagctgcg ctgccgtcgc aaaccgctga gcgcagcgtg
     6061 cagaagaaaa tttcgccccc ccgtttggag gagacccatg ctgcgacggt gcagaagccg
     6121 gtagagaaaa gaatcaaaac ggccgctgcg cataacgaag ctgcattgcc tcagccaaaa
     6181 ccggagacgt atgagaagaa ggaagcgaag ttctcgcctt tctttttttt cttgaagcac
     6241 aaggcccccg cagaaccgga tcctgtcccg gcgccagtag gaagtgcaat ggttatgctg
     6301 gctatcgccc catggggtga aatttatgtc gacggcgacc gggttggggt cagccccccc
     6361 gtaaatgaag tggaggtagc gcctggcaaa cgaacaatag aaatcaggaa cggtactttt
     6421 ccggtcttca cgcaagttct ggacgtgagg gccgatcaaa aaattcgcat caagcacaag
     6481 ttcgattaga taacatgccg tcctttcctg tcctcgctat tctgatgaca ctcctgttgg
     6541 ccgggtgcca gacggacggc ttgttcaagg gacaaggcgc aacgcgccat cctggcgata
     6601 gcttcctgca agaaggcata gaagattacg aagagggtaa ttatcgcacc gccaaacggc
     6661 ggttacagtt tgcgttggaa gaaggattaa gccgccccga tcgggtcaag gcgcataaat
     6721 atcttgcgtt tattgcgtgc gtctcgaacc agcaacttgc ctgccgtgaa gaatttgcag
     6781 tggcattgga gcttgatccg gcctttgaac tcagtgccgc tgaggccggc cacccgatct
     6841 gggggcccgt gttcaaaaac atcaaagcaa aaaagaccaa gcaaccatga acggcgaacc
     6901 aacaatcagc acgctgggcc gttacgaaat cctgtcggag ctcgggcagg gatcgatggg
     6961 ggtggtgtac cgggcccgcg atccgatgct ggatcgaatt gttgctatca agactatcaa
     7021 cctgacgctg ccaaaagacg aacttgcgga atatgaggca cgcttctatc aggaagccaa
     7081 ggcagcggga gggttgtccc atcgcaacat cgtaaccatc cacgatatcg gccgcagtga
     7141 gcgcgttgcc tatatggcaa tggaattcct ggaaggccag gaactgcgac gtctgttgca
     7201 ggcccggacg ccgattggta tggctcacgc acttgacgtc ggcgcgcaag tcgcggaagg
     7261 gctgcaatac gcacatgagg ggcacatcat tcaccgagac atcaagcccg ccaatatcat
     7321 ggtcttgaat gacgggctgg tgaaaataac ggatttcggc attgcacgca tgcgcaccaa
     7381 tgaagtgaag acgatgaccg ggatgattct tggctcgcca aaatacatgt ctccggaaca
     7441 ggtgtcgggc aagcgcgccg atacacgatc ggacattttc tcgctcggtg tggtgcttta
     7501 tgaaatgttg accggcgcgt cgccgttcgt ggccgataac atccatgggg tgatgtatca
     7561 aaccatgaat ttcaatccac cggcgccaaa gactttgaat cccgaactcc cggaggtgct
     7621 caatttcatt atcgcaaagg ccctggcaaa gaatctggat gatcgctacc agaaagcggc
     7681 agagttgagc aatgatttgc gccgggttat ctccgcgtta acgggggact cgggcgtccc
     7741 tcaggatgtc gtttcgggtc cacttccagc gcctttcatc gagcccatat ccccgctcac
     7801 gcggcaggaa gacaaacagg cgcgcattct cggaatggcg gatgacgccg atttgcacca
     7861 cgccgcagaa gtcccgagcc agccggagca gctaccatca gccgaatcca gtgtgcccgc
     7921 ccttggcctc tcaagggcgt ttgattcatt cgatgccacc atgcggcttg ccgccttaac
     7981 cggggtcgag aagcaattgg acaccttctc tgaaacgcaa aaaatcgcgc gcctgaaggc
     8041 gcttgcagat gcaaaacgtc aagcggcctc ggcggcggct tcaactctgt cgggcagcgc
     8101 gacctggaaa caactggcca cagaacgacc aaatcccgcg tcacccatgc cggtaaatca
     8161 agtgaaacta atctggctgg gcagcggcgc cctggccatc gcagccgcgt tactgtttct
     8221 gttgcggtga gccatatcag cgcactcttg ctacggcttc ttccacgcgc tctacactga
     8281 ccacttcgat tccatcgatg tttgcccgtg gcatattggc tttgggaatg atcgccaatt
     8341 tgaatccgag tttcgctgct tccttgagac gttcctgacc ccgctgaacc gggcgcactt
     8401 cgccggcgag ccctatttcc ccaaatgcga ccattttttc gggtagcgct ttgttgcgca
     8461 gcgacgacac gatggccagt aatactggaa ggtccgccgc gggttcatcg attttgacgc
     8521 ctccgactgc gtttacaaac acgtcttggt cgaacgccgc tatgccggcg tggcgatgca
     8581 gtactgcaag cagcatggcc agccggtttg gttcaagtcc gacactaagc cgccgcgggt
     8641 tcggtgcatg agcttcgtcg acaagggctt gaacttcgac aagcaacggc cgactgcctt
     8701 cctgagtaac catcacgcag gaacccgcta cctgggtgcc gtgctgggaa agaaacaaag
     8761 cagaaggatt tgaaatgccc ttcaacccgc gttccgtcat tgcaaataca cccagctcgt
     8821 tgactgcgcc gaatcgattc ttgatcgcgc gaatcaaccg aaaacttgag tgagtgtctc
     8881 cctcgaaata cagtacagcg tcgaccatgt gttcgagcac cctcggacca gccagggcac
     8941 cttccttggt cacatgcccc accaggagca gtgcagtacc ggatgatttt gcgaatcggg
     9001 tcaattgcgc agcgcattcg cgcacctgtg ccacgcttcc gggtgccgac tgaagcgcct
     9061 ctgaataaac agtctggatc gagtcgacca ctgctacgtc gggtttttgt gtggccaaca
     9121 tcgacattac tttttccagt tctatttccg ccatcaaatt cagcgaggcc gcgtttagcg
     9181 ccagccgacg cgcgcgtagt gcgatctgct gtacggactc ctctccgctc acgtaaatcg
     9241 cgttgcagct gttgccaagt atcgacaggg cctgcagcag cagggttgat tttccaatgc
     9301 cggggtcgcc gccgatgagc accacagcac ccggcaccag acctccgccc agcactctgt
     9361 caagctcatc tatgccggtc ggcctgcgag gctcttctcg cgcatcaaca tccgcgatct
     9421 tctgcagctt gctggatttc gcgacagact gatagcgcga gccgcctgct ttttccgcga
     9481 tgctttcgac cagggtattc caggcagtgc agtgagggca ctggccttgc cacttgaggg
     9541 tttgcccgcc gcattcggta cagctgtaga tcgtttttgg cttggccatg gcagtcagac
     9601 cttttctcga accggtactc ttgcggccaa tgcgcacatc aactcgtaac tgaccgtgcc
     9661 ggcatgcccg gcgacctctt cgatcggaag gccctctccc catagcacgg ccggactacc
     9721 gactcgcgca tggggaacat gcgtgagatc gacgcacagc atgtccatcg atacacgccc
     9781 ggcaatcgga acacgcgtgc cttctatcag caccgacacg ccattggcgg cctgccgggg
     9841 atacccatcg gcgtagccac aggcgataac accgatgcgc atatcgtgat ccgccgtaaa
     9901 tacgccgccg tagccaacgg tatcgcccgg tttgagtttt tgaattgcaa tgacttcgct
     9961 ttttaaagtc attaccggtt tcagtccgag tttttctgcg ctgatatcgt gaagggggct
    10021 ggcgccatag agcatcagcc ccgggcgcac ccaattccca cgcgcttccg ggaaacgtag
    10081 cagagccgcc gagttcgcca gtgaacgttc gtaattcggg cgggcaacgc atgaatcgaa
    10141 ttcacgcatt tgcttttgga taccgcccgg tccttccgcg t
//
LOCUS       DATMZQ010000200         4778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4912826, whole genome shotgun sequence.
ACCESSION   DATMZQ010000200 DATMZQ010000000
VERSION     DATMZQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4912826"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VN496_06390"
     CDS             <1..727
                     /locus_tag="VN496_06390"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16613.1"
                     /translation="GDAARFGEAARAVTRTKAVIALIGGRTAGGRDAAFRHTGSRPAD
                     DAATDEFCRRSGIVRVRSLRRLLLAGKAFGAFPLGIGKRVLLLSNSGGPGVLAADQAL
                     DEGLVLPELPVAMAERLLKILPAEASVANPLDLLADARAERFHSTLAAALELGEQQFD
                     AILMIHVVPFMVDAGEIVEALAALSKTARVPIMHCMMGTLEHKDDWFARLEQSGVPTF
                     KDAEEMCIAAGALARHRELNAVR"
     gene            758..1396
                     /gene="maiA"
                     /locus_tag="VN496_06395"
     CDS             758..1396
                     /gene="maiA"
                     /locus_tag="VN496_06395"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HXF16614.1"
                     /translation="MKLYGYFRSSASYRVRIALAMKGLDYEQASIHLAKGRQYATEFS
                     AVSPQNLVPVLEHDSQKFYQSLAIIEYLDEKFPNPPLLPADTSARSRVRSLALITACE
                     IHPLNNTRVLAYLTETLRVSDEQKNLWYSHWVTLGFSALEKRLATEKQTGRFCHGDMP
                     GLADIVLVPQVANANRFKVNLDGFPTIRRINEHCLALEPFKKAMPQNQPDAE"
     gene            1450..1878
                     /locus_tag="VN496_06400"
     CDS             1450..1878
                     /locus_tag="VN496_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16615.1"
                     /translation="MKPKKIWVAAGFLFALAMPLAGAADEVEAPKIHKQGGSTYATGG
                     IGEAQRKALFKIAPKFPIQLIFEVEGEQTDISGVKVTLTDQSGNSLIEAVSEGPYFYM
                     NPPAGGRFIIDAEYKGEKQTMTKDLVGRRYLVLEFKFHAK"
     gene            1882..2217
                     /locus_tag="VN496_06405"
     CDS             1882..2217
                     /locus_tag="VN496_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16616.1"
                     /translation="MAIELLAKPVVDALLKALDRAKDANLRASARKAIAEAISELILI
                     SPDLSKAKAKIAIARAAGILDQDLFTAESMLKKVKKANRTAKSGEPKKSAPKRRKRKV
                     AAKRRRVRS"
     gene            2312..2620
                     /locus_tag="VN496_06410"
     CDS             2312..2620
                     /locus_tag="VN496_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16617.1"
                     /translation="MNRTVVSSLIALVLAGASASVLAEGATNGRERFFLKRQNCAWTE
                     EMGKYIYECIKKNDGFNAHWCHNEAIDAFCPPDENAIPHPTDVQPSEKPAEPEAGKKG
                     "
     gene            complement(2690..3343)
                     /locus_tag="VN496_06415"
     CDS             complement(2690..3343)
                     /locus_tag="VN496_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16618.1"
                     /translation="MKFAGVLLGLIVLAGVLIVWFRAYKSIKSDQALSPSGKGAPKQK
                     KDSLDDFIASYKRGEVSLENNTKVLPSPAAPGTPAANSVLSPPFTPPAPAAIAAAAVD
                     AGPIKCESFLAGATKLAYLTAKTGLRDHHIFAHVQLAALSTGAPLAEPLSHASVDLVI
                     CNAALAPVAAIDLIDAASGPAPVAKSEHLRMLGVRYLRLSVKSLPKPDEWHTLLYKM"
     gene            complement(3344..3868)
                     /locus_tag="VN496_06420"
     CDS             complement(3344..3868)
                     /locus_tag="VN496_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16619.1"
                     /translation="MVFDYYKKLSAKRQSIYRQSDAINSVKLPDAELLRSPALALVDA
                     LKSEKRTTVQLLSQQVTDGIVGQLSVPPLRIQVLAVRPSNDWGELHGLYLPEEDGKIA
                     KVQLWMRTAKHKRVVAFKSYLRTLLHEICHHLDYELYQFPETFHTEGFYNRESSLFRQ
                     LVPADQADARRLGT"
     gene            complement(3876..4559)
                     /locus_tag="VN496_06425"
     CDS             complement(3876..4559)
                     /locus_tag="VN496_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXF16620.1"
                     /translation="MIGDPVALTIRRNIDRPSNAIMKAFAGMPTGFVTDAYNGKGCLD
                     YLIKPLLPTMQFSGSAITAFCGPMDNLAAMAILDFAQPGDVIVIATTGDDTAATIGDL
                     WAFWAKRLGVAAIVCDGLVRDVAGLLKADIPVFARGIKPNSAFKNGPGEVNTTVTCGG
                     VAISAGDIIVGDRDGIVAVPLAQAAQVVERLELVKQKEAEAEAKVKSGVKLKFWDPDA
                     LGRRVQYLD"
     gene            complement(4596..>4778)
                     /locus_tag="VN496_06430"
     CDS             complement(4596..>4778)
                     /locus_tag="VN496_06430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXF16621.1"
                     /translation="EKGMEGMKPGGRRTVIIPAKNAYKGARQEPPDGVPIDAKLIIDI
                     ELVDVVPQANSAPANE"
BASE COUNT         1046 a         1350 c         1415 g          967 t
ORIGIN      
        1 tggggacgcg gcccgctttg gcgaagccgc acgtgccgta acaaggacaa aggcagtgat
       61 agcgctcatt ggcgggcgta cggccggtgg gcgagatgcc gcctttcgcc acaccgggtc
      121 gcgaccggcg gatgatgcgg cgacggatga gttctgccgc cgcagcggca tcgtcagagt
      181 ccggagcttg cggcgtttac tgcttgccgg gaaagcgttt ggcgcatttc cgctggggat
      241 cggcaaacgt gtattgctct tgtccaattc cggcggtccg ggtgtgcttg cagccgatca
      301 ggcacttgat gagggactgg tgctgccgga gctgcctgtc gcgatggcgg aacgactgct
      361 gaagattctg cctgccgagg catcggttgc caatccgctc gatcttctcg ccgacgcccg
      421 tgcagagcgc tttcactcaa cgctcgcggc cgcgctggaa ttgggcgaac aacagttcga
      481 tgccatcctc atgatccatg tcgtcccctt catggtggat gctggcgaga tcgtcgaagc
      541 gctcgccgca ttgtccaaga cggccagggt gcccatcatg cattgcatga tgggcacgct
      601 cgaacacaag gacgactggt ttgcgcgcct ggagcaaagt ggcgtgccaa cattcaagga
      661 tgcagaagaa atgtgcattg ctgccggcgc gctggcgcgc catcgcgaac tcaacgcggt
      721 acgctgatcg gacgtaacaa cgggagagcc aacaatgctg aaactctatg gttacttcag
      781 aagttcggcg tcttaccgcg tgcgtatcgc gctcgccatg aagggtctcg actacgagca
      841 ggccagcatc catctggcca aaggcaggca atacgcgacc gagttttctg ctgtcagccc
      901 gcagaacctg gtgccggtgc tcgagcacga cagccaaaag ttctatcaat cgctggccat
      961 catcgaatac ctggatgaga aattcccgaa cccgcctctg ttgccagccg atacttcagc
     1021 acgcagccgc gtgcgttcgc tggcgctgat cactgcctgc gaaattcatc cgttgaataa
     1081 cacccgcgtg ctggcttatc tgacggaaac actccgggtc agcgatgaac aaaaaaacct
     1141 ctggtacagc cactgggtca cgctcggatt tagcgcgctg gagaaaagac tggccacgga
     1201 aaagcagacc gggcgttttt gtcacggcga catgcctggt ctggcggata tcgtgcttgt
     1261 gccgcaggtt gccaacgcga atcgattcaa ggtcaacctg gatgggtttc caaccatccg
     1321 tcgcatcaac gaacattgtc tcgccctgga gccattcaaa aaggcgatgc cgcagaatca
     1381 gcccgacgca gagtaggcgc gcgcggcagg cgtgatattc tgagctttgc gttgacagaa
     1441 agactgaaga tgaagccaaa aaaaatctgg gtagcggccg gctttttgtt cgcgttggcc
     1501 atgcctttgg ccggtgcagc cgacgaagtt gaagcgccca agatccacaa gcagggcggc
     1561 tcgacttacg cgaccggtgg catcggggaa gcgcaacgca aggcgctgtt caagattgcg
     1621 cccaagtttc cgatccagct gatattcgaa gtcgaaggcg agcagaccga catcagcggc
     1681 gtgaaagtta cgctgaccga ccagagcggg aattccctga tcgaggcggt ttccgaaggc
     1741 ccttatttct atatgaatcc tcccgcgggc gggcgtttca tcatcgatgc ggaatacaag
     1801 ggtgaaaagc agaccatgac gaaggatctg gtagggcggc gttacctggt cctcgaattc
     1861 aagttccacg ccaaataatc catggcgatc gagctgctgg cgaaaccagt cgtcgacgcg
     1921 ctgctgaagg cgctggatcg cgccaaggac gcaaatttgc gcgccagtgc ccgaaaggcc
     1981 atcgccgaag cgatctccga actgattctc atcagtccgg acctcagcaa agcgaaagcc
     2041 aagatcgcca tcgcgcgggc agctggcatt ctcgatcagg atctgttcac ggccgagagc
     2101 atgctgaaaa aggtgaagaa agcgaaccgg acggcaaaat ccggcgagcc gaagaagtcg
     2161 gctcccaagc ggcgcaagcg aaaagtggcc gccaagagac ggcgcgtccg cagctaagca
     2221 aagccccgat atccggggcg ggcgttttac cctaaaatcg aaacctttgc tgagccaacg
     2281 aggtcagtac tgcatactga acaggatgac gatgaacaga acagtcgtat caagcctcat
     2341 cgctttggtg cttgccggtg caagcgcatc ggtattggcc gaaggggcga ccaatggccg
     2401 cgaacgcttc tttctcaaac ggcagaactg cgcctggacc gaagaaatgg gcaagtatat
     2461 ttacgagtgc atcaagaaga acgacggctt caatgcccac tggtgccata acgaggcgat
     2521 cgacgcattc tgcccgccag acgagaatgc cattccgcat ccgaccgacg tgcagccgag
     2581 cgagaaaccc gctgagccgg aagcaggcaa gaaaggttaa acgtcaagcc acgcgaacaa
     2641 ggccgcttcc agcggccttt tttgtcagat acggcgaatg aatggccggc tacattttgt
     2701 acagcagcgt atgccattca tccggcttgg ggagcgattt gaccgagagc cgcaggtaac
     2761 gaacccccaa catcctcagg tgttctgact tggcgacggg cgccgggcca cttgcggcat
     2821 cgataagatc gatggccgcg accggagcga gcgccgcatt gcagatcacc aggtcgacac
     2881 tcgcgtgtga gagcggctca gcaagcggag cgcccgtcga gagcgcggca agttgaacat
     2941 gcgcaaagat atggtgatcg cgcagtccgg tctttgcagt caggtaagcc aactttgtcg
     3001 cacccgcgag gaacgactcg catttgatgg ggcccgcgtc caccgctgca gcggcaatgg
     3061 ccgcgggtgc cggcggagtg aatggcggcg acaagaccga atttgcagcg ggcgtccctg
     3121 gcgctgcggg agaaggaagc accttggtat tgttttccag gctcacttct ccgcgcttgt
     3181 acgaggcaat gaagtcgtcg agtgaatcct tcttttgttt cggtgcgccc tttcccgaag
     3241 gcgacaacgc ctgatcgctc ttgatcgact tgtaggcccg gaaccagacg atcaagacgc
     3301 cggccaggac aatcaaacct aacaggacac ccgcaaattt cattcaggtg cccaggcgcc
     3361 gagcatcggc ctggtctgcc ggcacaagtt gacgaaacaa gctggattca cggttgtaga
     3421 aaccctcggt gtggaacgtt tccggaaatt ggtaaagttc atagtccaga tggtggcaaa
     3481 tctcgtgcag taacgtgcgc aaatagcttt tgaacgcaac gacgcgtttg tgcttggcgg
     3541 tgcgcatcca caattgaacc ttcgcgatct tgccgtcttc ttcgggcagg tacaagccgt
     3601 gcaattctcc ccaatcgttg ctcggcctta ccgcaaggac ctggatacgc aaaggtggca
     3661 cgctcaattg tccgacaatg ccatccgtca cctgctgcga gagtagctgg acagtggtgc
     3721 gtttttcgct cttcaaggca tccacgagcg ccagcgccgg cgagcgcagc agttcagcat
     3781 cgggcagttt gactgagttg attgcatcgc tttgccggta gatgctttgt cgtttggccg
     3841 aaagtttttt gtagtaatcg aaaaccaagt taatctcaat cgaggtactg cacccggcgc
     3901 ccaagcgcat ccggatccca aaacttcagc tttacgccgc ttttgacttt ggcttccgcc
     3961 tctgcttctt tctgcttcac caattcaagg cgctctacga cctgtgcggc ctgagcaagc
     4021 gggacggcta cgataccgtc gcgatcgccg acaatgatat cgccggccga gattgcaacg
     4081 cctccgcatg tcaccgtcgt gttgacttcg ccggggccat tcttgaaagc ggaattcggt
     4141 ttaatgccgc gcgcgaacac cgggatgtcc gctttcagca aaccggcaac gtcgcgcacc
     4201 aggccgtcgc agacaatagc ggcgacgccg agcctttttg cccagaacgc ccacagatct
     4261 ccaatggtgg cggcagtatc gtctcccgtc gtggcgatta cgatgacatc gcccggttgc
     4321 gcaaagtcaa gaatggccat ggccgcgaga ttgtccatcg gtccgcagaa tgcagtgatc
     4381 gcactgccgc tgaactgcat ggtcggcaaa agcggcttga tcaagtagtc gagacagcct
     4441 ttaccgttat aggcgtccgt gacgaagccg gtaggcatgc ctgcaaacgc tttcatgatg
     4501 gcgttcgagg gtcggtcaat gttgcggcgg atggtcagtg caacaggatc gccgatcata
     4561 cggttaatgc tcctttttgt ttgttgttct ttcgattact cgttggcggg ggcggagttg
     4621 gcctgcggta cgacatcgac gagttcaatg tcgataataa gcttggcatc tattggcacg
     4681 ccatccggtg gctcctgccg agcgcccttg taggcgtttt tggcgggaat gatgacggtg
     4741 cggcggccgc cgggtttcat gccttccatg cctttttc
//
LOCUS       DATMZQ010000201         2814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_155692, whole genome shotgun sequence.
ACCESSION   DATMZQ010000201 DATMZQ010000000
VERSION     DATMZQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155692"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VN496_06435"
     CDS             <1..965
                     /locus_tag="VN496_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HXF16622.1"
                     /translation="ISGSLTQETDMRYALHAEASPDASSTRMAKDPPGGGAGRYRADH
                     YDVIGNVRNLPIRIFTLGQFSIFRNAVAITSKGKAQHRPLGLLQALIALGGKDVAGSR
                     LCECLWPDSDGDLAGRNLNITVHRLRQILQAPGVVVQRDGKLTLSSEHCTVDALHFER
                     LVSNSIEGLGNAAMAEDAESNLHAAMGLYCGHFLALESEEPWMLAPRLRMKSKFERLV
                     LALSRHHEQQMRFEQAVDICRRALELDPLNELLYRCLMGCYLKQGEIARALDTYVRCR
                     EALAKGLSAAVSCETARLYVQVLREAQNGNHHSYNLAAKYRPHV"
     gene            1191..1772
                     /locus_tag="VN496_06440"
     CDS             1191..1772
                     /locus_tag="VN496_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HXF16623.1"
                     /translation="MKTLGTVLIASALALSTSITYAADNESILRLRAIQVEPDVGGDV
                     QGLDVDGATGGEVAFTGFMSEHWAIELGIGTTKHDLTINGANAGSVKLMPVNLMLQLH
                     FLRDAIIRPYIGAGGNYTRFYEVNFAGGSADVDRDRYGPVAQVGLDVPLGKSFLLNAD
                     VKKIWLNTDVSGAANGTIDLDPWVYALGIGFRF"
     gene            complement(2009..2533)
                     /locus_tag="VN496_06445"
     CDS             complement(2009..2533)
                     /locus_tag="VN496_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16624.1"
                     /translation="MKNSFSGKSKLLSAAVAALFTVTAYSALADNAVKAGGPSDKPGR
                     TIDDSVKAPGNPDRPGRALDDGKVTSQGVAKRPGRAVDEGTVTSKGAATTPGRTADED
                     VVKSGGAHKQARAIDDVKAGGKTTDTQGRSLDENKKVKKGKKTTSAPGRAVDESTVKT
                     QGPSDKASRALKKE"
BASE COUNT          602 a          774 c          799 g          639 t
ORIGIN      
        1 cgataagcgg ctccctgacg caggagacgg acatgagata cgcgctgcat gcagaggcca
       61 gcccggatgc ttcatccacc agaatggcaa aggatcctcc gggtggtgga gcaggacgct
      121 accgggcaga tcattacgac gtcatcggga acgtcaggaa tttaccgata cggattttta
      181 cgcttgggca attctccatc tttagaaatg cggttgccat tacgtcgaaa ggcaaggcgc
      241 agcaccggcc gctaggcctg ctgcaggcgt tgatcgcgct gggcggaaaa gatgttgccg
      301 gcagccgcct atgcgaatgt ctctggcccg attccgacgg ggatctggct ggacgaaatc
      361 tgaacatcac cgttcacagg ttgcgccaga tcctgcaagc gccgggcgtt gtcgttcaac
      421 gcgacggcaa actgacgctt agttcagaac actgcacggt tgacgccctg catttcgaac
      481 gcctggtaag caacagcatt gaaggcctcg gtaacgctgc aatggcggaa gatgcggaaa
      541 gtaatcttca cgctgccatg gggctatatt gcggccattt tctcgcactc gaatccgaag
      601 aaccctggat gctggcgcct cgcttgcgca tgaagagcaa attcgaacgc ctcgtgctgg
      661 cgctgtcccg gcaccacgag cagcaaatgc gatttgagca agccgtcgat atttgccggc
      721 gcgcccttga gctggatccg ctcaatgaat tactgtatcg ctgcttgatg gggtgctatc
      781 tgaagcaggg tgaaatcgcc cgcgcgctcg atacctatgt gcgttgtcgc gaggccctcg
      841 caaaagggct atccgcggcg gtgtcctgtg agacggcgcg tctttatgta caggtgctcc
      901 gggaagcgca aaacggaaac caccatagct acaatctagc ggccaaatac cgcccgcatg
      961 tttgacgaga tgctggttat tcgtccataa cagagcgtgc tcatcaccag ataactgtct
     1021 gctggcggcg acattcgtga caaatctgct gaaacttttg ctaccaggcc ggcgtctatc
     1081 tggcgaaggc ggcatagaac gtccatgacg gacaagacag ttcgatctcg tcctacaggg
     1141 ggttggtttc ttttagcaaa ctaaaatcaa tttctttgca ggagtatcaa atgaagactc
     1201 tgggaactgt tctcatagcg agtgcgctcg cgctatcaac atcaataacg tatgcggccg
     1261 ataacgaatc aatcttgcgg ctaagggcaa ttcaggttga gccggacgtg gggggcgatg
     1321 ttcaaggcct ggatgtcgat ggagcaacgg gcggagaagt cgccttcact ggattcatgt
     1381 ccgagcattg ggccattgag ctggggatcg gcacgaccaa gcacgatttg actatcaacg
     1441 gtgcgaacgc aggaagcgtc aaattaatgc cggttaatct gatgttgcag ctccacttcc
     1501 tgcgggatgc cattattcgt ccgtacatcg gcgccggcgg caactacact cgcttttacg
     1561 aggtaaattt tgccgggggg tcggcagatg tagatcggga cagatatggt cctgttgcgc
     1621 aagtcggtct cgatgtcccg ctcggcaagt catttctgtt gaacgcagac gtgaagaaaa
     1681 tctggctgaa cacggacgtg agcggagcag cgaacggcac tatagacctc gatccgtggg
     1741 tgtacgcact cggtattgga ttcaggttct gaccgcaata gcttgcaagc ggtgtgaggg
     1801 caaagaaaaa ggcccgctga ttacagcggg ccttttaacg cgtgcggaag agatcctccg
     1861 cgggcagctt aattcggcat tggaccggta aacaatgaac ggcggatttc cggcggaaat
     1921 ttcttccaga tgcgcacgct tcaaccaacc gccaattgaa aagggccgac cacggcgtgt
     1981 cggccccttc ggttgccctg ccccaacact actctttctt caatgcacgc gacgccttgt
     2041 ccgatggacc ttgtgtcttt actgtggact catcaactgc ccggcctggc gcagacgtgg
     2101 tcttcttacc tttcttcacc ttcttgtttt cgtccagact ccggccttgt gtatcggttg
     2161 ttttgccgcc ggccttcaca tcgtcgattg cccgggcttg cttgtgtgct ccacctgact
     2221 tgaccacgtc ttcgtctgcg gtccggcccg gtgtcgtcgc tgctcctttt gatgtcacgg
     2281 taccttcgtc gaccgcacgg cctggcctct tggccacgcc ctgcgaagtt accttgccat
     2341 catccagtgc acgtccggga cgatccggat tgcccggtgc cttgacgctg tcatcgatgg
     2401 tacgccccgg tttatcggat ggcccgcccg ctttgactgc attgtcggcg agggcagagt
     2461 atgcggttac tgtaaacaat gcagcaactg cggcgctgag taacttggat ttgccactaa
     2521 atgaattttt catgttggtt cctctcgagg gaaaaacaat gagcacatgc ccacgagtcc
     2581 agaataggga accacggcag cagagtcccg tgaaaattca cgccgcgtgg cgtgaaaatt
     2641 catatcgcaa actacttgct ggaaatggcg cctcggttgg ccatcggctc ggacattctc
     2701 gctaccacgc gcgtggaggg cttttggcga tgatgatccg ctcaccctcc atacggacat
     2761 aacccccggt gcggagatcc ttcaggatgc ggctgatcat ttcgcgcgag cacc
//
LOCUS       DATMZQ010000202         5715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3532305, whole genome shotgun sequence.
ACCESSION   DATMZQ010000202 DATMZQ010000000
VERSION     DATMZQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532305"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VN496_06450"
     CDS             <1..997
                     /locus_tag="VN496_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16625.1"
                     /translation="ISEASMGVPVKATMDTVIVGNLEDGTPVHMDRFAAEAEGIVVIN
                     RIKPHTAFRGATESGVTKMLSIGIGKINGAATYHQHGMDTFPVLLPKVRDVNLARRNV
                     LLGVGIVENAHDQTAVIEIMPAAQIATREPVLQELAKRQMPQLYFDEIDVLVIDEMGK
                     NISGAGFDPNITGRNRRFVKWNFGPKVKKIVVLGLTPETHGNATGMGGADIITMRLFR
                     EIDIPSTYANIITSMNLDGGAIPIVMNTEREAIQLAVKTVVRVKPENCRIVRIANTLE
                     LAQIQVSEPMLGEVRARPEQFQIASPAAAFVFDSEGRLAPLPSGHEASAVAMALR"
     gene            1120..2118
                     /locus_tag="VN496_06455"
     CDS             1120..2118
                     /locus_tag="VN496_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXF16626.1"
                     /translation="MSAHLRVGFAGAGAISQYHLVGWSAMPDASVVAICDIDESKARA
                     KAQAFGIPKVYTDFQKMIGSENLDAVDIVTPVGTHAPLTRLAADAGLHVCCQKPLTPT
                     VHEAEALIAYVGERVRFMVHENYRFRPHYVLIREWLKQGRIGDIKQARMTVRCQGMMQ
                     VGDVQPFLLNRQPYLKDFARLLIFEVLIHNLDVMRVLLGPLQVVAAHIAKVNQQLSGE
                     DAAVVVLKGAGGITAVLDGNISAPGYGPLPTDRLEIMGTRGTLLFDRDRLMMVGSDDP
                     PIVFDLAQNYQACFSSAVAEFARGLRENKPFAMDRLDNLETLKLMEQSYIAAGMKI"
     gene            complement(2128..3330)
                     /gene="pcaF"
                     /locus_tag="VN496_06460"
     CDS             complement(2128..3330)
                     /gene="pcaF"
                     /locus_tag="VN496_06460"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HXF16627.1"
                     /translation="MTEAFICAALRTPIGRYGGALSSVRADDLAAVPLRELMARHHSV
                     DWNAVDDVVYGCANQAGEDNRNVARMALLLAGLPKEVPGSTINRLCGSGMDAVGIAAR
                     AIKAGEAAFVIAGGVESMSRAPFVMPKADSAFARTNAVYDTTIGWRFINPLMKQLYGT
                     DSMPETAENVAAEFGITRARQDAFAFRSQQKALAAQSSGRLAKEIVAVKILVKKGDAV
                     LMERDEHPRETSLEALSKLKGVVKPDGTVTPGNASGVNDGACALIVASEAAAVRHGLT
                     PLARVIGVATAGCAPRIMGMGPVPATQKLLARTGTKLDQVDVIELNEAFAAQGVAVLN
                     ELGIADDDPRVNPNGGAIALGHPLGMSGARLVTTAIYEMRERGSQRALATMCIGVGQG
                     IALLVERV"
     gene            complement(3381..4553)
                     /gene="dgoD"
                     /locus_tag="VN496_06465"
     CDS             complement(3381..4553)
                     /gene="dgoD"
                     /locus_tag="VN496_06465"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HXF16628.1"
                     /translation="MKITAIKTAVCNAEMRNWVFVRVETDQPGLVGWGEATLEWKTRG
                     VVGAVQDLEPLLVGRDPRDIEQAVRIMFKHSFWRLGVIGNTAVSGIEMALWDILGKVL
                     NVPVWRLLGGKTHDKIKVYTHLGFGDMTSVYESMDHGAVVERGLQVIDKGYRALKVVF
                     IPYTHFTASPKQQDHVGGMMQKLREAVGDEIEIMIDFHGRPASTSAALQYIHALVPSR
                     PLFVEEPIQPGDAPGMKQILDKSPVPIAAGERLVDRREFDDLFRLRAINIAQPDLCHV
                     GGFTEARKIAAMADTAGIGIAPHNPLGPLAGVAALHFDIATPNFVIQEEMSGAVPWYH
                     EVVRGPIRMQDGFWQVPEQPGLGVEVDEKAIARHPFKQEIMHAGHAVLDDGTIVDW"
     gene            4667..>5715
                     /locus_tag="VN496_06470"
     CDS             4667..>5715
                     /locus_tag="VN496_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXF16629.1"
                     /translation="MGPFDYIIVGAGSAGCLLANRLSADANIRVLLLEAGGKDDYFWI
                     HVPVGYLYTINNPRTDWCYRTQPDEGLNGRSLNYARGLGLGGCSSINAMIYMRGQKSD
                     YDYWAALGNRGWSWDEVLPYFKRTEDYAHGADDLHGAGGELRVEERRVHWEILDAWRD
                     AAEECGIPKIKEFNRGDNFGNAYFQMNQKRGVRWSNTKAFLRPVLHRPNLTVLTHAHV
                     NRIRFDTSAGHRRATGIEFRTRDGANQFAQARREVILAAGAIGSPQILQLSGVGPGAV
                     LQKCGVPVIHELPGVGENLHDHLQIRIMYKVSNTVTLNERTHSLFGKLAMGLEYLFYR
                     TGPLTMPPSQLGAFA"
BASE COUNT         1191 a         1741 c         1647 g         1136 t
ORIGIN      
        1 gatttccgaa gcgtccatgg gagtgccggt aaaggccacc atggataccg tcatcgtcgg
       61 caacctcgaa gatggcacgc cggttcatat ggatcgcttt gcggccgaag cggaaggcat
      121 tgtggtgatc aaccgcatca agccgcacac cgcgtttcgc ggcgcaacgg aaagcggcgt
      181 gaccaagatg ttgtcgatcg gtatcggcaa gatcaacggc gcagccacgt atcaccagca
      241 cggcatggat actttcccgg tgctgcttcc aaaagtacgt gatgtcaatc tcgccaggcg
      301 taatgtcctg cttggcgtgg gcatcgtgga aaacgcgcat gatcagaccg cggtcataga
      361 aatcatgccc gctgcgcaga ttgcaacccg tgaaccggtg ttgcaggagc ttgcgaagcg
      421 acaaatgccg cagctgtatt ttgatgaaat agatgttctg gtcatcgatg aaatggggaa
      481 gaacatcagc ggggctggct tcgatcccaa cattacgggc cgaaatcgcc gcttcgtgaa
      541 atggaacttt ggcccaaagg tcaagaagat cgttgtcctt ggcctgacac ccgaaacgca
      601 cggcaatgca accggaatgg gcggcgcgga cattattacc atgcgactgt tcagggaaat
      661 cgacattccg tccacatacg cgaacatcat cacatccatg aatctcgatg gcggcgcaat
      721 cccgatcgtc atgaacactg agcgtgaggc catccaactg gcagtcaaga cggtcgtgcg
      781 cgtcaagccc gaaaactgcc gcatcgtacg catcgcgaac accctggaac tggcgcaaat
      841 acaggtatcc gagccgatgc ttggcgaagt gcgtgccagg cccgagcagt ttcagatcgc
      901 atctccggct gctgcatttg ttttcgatag cgaagggcga ctcgctccgc tgccatcggg
      961 acatgaagca tcagccgtag cgatggcctt acgttgagtt gcgatctgtt gagaaaaatt
     1021 aaagaccgcg acctatccca agtgaagcgg agcagcacgg acgaatcgaa accctgccgc
     1081 ggcagggttt ttctttaggc gcttagggat cggaacgaaa tgtctgcaca ccttcgagtt
     1141 ggctttgctg gcgccggcgc catcagccaa tatcacctcg ttggatggtc cgctatgccg
     1201 gatgcgtcgg tggttgccat ttgcgacatc gatgaaagca aggctcgggc gaaagcgcaa
     1261 gcgtttggaa ttccaaaggt ctacaccgac ttccagaaga tgatcggcag cgagaacctt
     1321 gatgctgttg atatcgtcac gccggtcggc acccacgctc cgctgacccg gctcgcggcc
     1381 gatgccggat tacatgtttg ctgccagaaa ccactgaccc ccacggtgca cgaggccgag
     1441 gcactcattg cgtatgtggg cgagcgcgtg cgattcatgg tgcacgagaa ctaccgcttt
     1501 cggccacatt acgtgctgat ccgcgaatgg ctgaagcaag gccgcatcgg tgacataaaa
     1561 caggcgagga tgaccgtgcg gtgccaaggc atgatgcagg tcggcgatgt gcaacccttc
     1621 ctgctcaatc gccagcctta tctgaaggat ttcgcgcggc tgctgatttt tgaagtgctg
     1681 attcacaatc tcgatgtcat gcgggtgctg cttgggccgc tgcaggtcgt cgccgcgcac
     1741 atcgcaaaag tgaaccagca actcagcggt gaagacgcgg cggttgtggt gctgaaaggc
     1801 gccggaggca tcaccgcggt tctggacggc aatatttcgg ctccgggtta cggtccactg
     1861 ccgaccgatc gccttgaaat catgggtacg cgcggcacat tgctgttcga tcgcgaccgg
     1921 ctgatgatgg tcggatcgga cgacccaccg atcgtattcg acctggcaca gaactaccag
     1981 gcttgcttca gcagcgcagt ggcagaattt gcgcgtggat tgcgcgagaa caagccgttt
     2041 gccatggatc gcctggacaa cctcgaaacg ctcaaactga tggagcagag ctatatcgcg
     2101 gccggcatga aaatctgatt cggacgctta aacccgttcc accagcaatg cgatgccttg
     2161 cccaacgccg atgcacatcg tggccaacgc acgctgcgac ccccgctcgc gcatttcgta
     2221 aattgcggtc gtcaccagcc ttgccccgct catgccgagc ggatgtccca acgcaattgc
     2281 gcctccgttc ggattgacgc gcgggtcgtc gtcggcaatc cccagttcat tcaacaccgc
     2341 gacgccttgg gccgcgaacg cctcgttcag ttcgatcacg tcgacctgat cgagtttggt
     2401 gccagttctc gccagcaatt tctgcgtcgc gggcaccggc cccatgccca tgatacgagg
     2461 cgcacatccg gcggttgcga cgccgatcac ccgggccagc ggtgtcaggc catgccggac
     2521 agcggcggcc tcgcttgcga cgatcaatgc gcaagcgccg tcgttgacac ccgaagcatt
     2581 accgggtgtg accgttccat ccggcttcac cacgcccttc agtttcgaca acgcttcgag
     2641 gctggtttcg cgcggatgtt catcgcgctc catcagcaca gcgtcacctt ttttcaccag
     2701 gatcttgacc gccacgattt ccttcgccaa ccggccgctg ctctgtgcgg ccagcgcttt
     2761 ttgctgggag cgaaacgcga acgcatcttg cctggcgcgg gtaatgccga attctgccgc
     2821 gacgttttcg gcggtctcgg gcatggaatc ggttccgtat agctgcttca tcagtggatt
     2881 gatgaaacgc cagccaatgg tcgtgtcgta gacggcattc gtgcgcgcga atgcactatc
     2941 ggcttttggc atgacgaatg gtgcgcgcga catgctttcc actccgccag caatcacgaa
     3001 cgcggcttca cctgccttga ttgcacgcgc cgcaataccg accgcatcca tgcccgagcc
     3061 gcacaggcgg ttgatggtgc ttccgggcac ttccttcggc agtccggcca gcagcaacgc
     3121 catgcgcgcg acgttgcgat tgtcctcgcc tgcctggttg gcgcaaccgt ataccacgtc
     3181 atccacggca ttccagtcca ccgaatggtg tcgtgccatg agctcacgca gcggaacagc
     3241 agcaagatcg tccgctcgga ctgacgacag tgccccgcca tagcggccga tcggcgtcct
     3301 cagcgcggca caaataaaag cttcagtcat gtctgtctcc cttccatttc agcgatcgag
     3361 ttgatgtgcg aagcgctgac ttaccaatcc acgatcgtgc cgtcatccaa cacggcatgg
     3421 ccggcgtgca tgatctcctg cttgaacgga tgccgcgcga tggctttttc gtcgacctcg
     3481 acaccgagcc ctggctgctc cggaacctgc cagaagccgt cctgcatgcg aatcggtcca
     3541 cgcacgactt cgtgatacca cggcaccgca ccggacattt cttcctggat cacgaaattc
     3601 ggcgtcgcga tgtcgaaatg caacgcggcg acgccggcaa gcggaccaag cggattatgt
     3661 ggagcaatcc cgatccccgc cgtgtctgcc atggctgcga tcttgcgtgc ttcggtgaag
     3721 cccccgacgt ggcacagatc aggttgcgca atgttgatcg cgcgcagccg gaacaagtcg
     3781 tcgaattcgc gccggtctac caggcgctct ccggccgcga tcggcaccgg agacttgtcg
     3841 aggatctgct tcatgcccgg cgcatcgccg ggctggatcg gttcttcgac aaacaggggc
     3901 cgggacggga ccagcgcatg gatgtattgc aaggccgcgc tggtcgacgc gggacggcca
     3961 tggaaatcga tcatgatctc gatttcatcg ccgactgctt cgcgcaattt ctgcatcatg
     4021 cctcccacat ggtcctgctg tttcggagac gccgtgaagt gcgtgtacgg gataaacacg
     4081 accttcaacg cacggtaacc cttgtcgatc acttgcagac cgcgctccac caccgcgcca
     4141 tggtccatgc tttcatacac ggacgtcata tcgccgaagc ccagatgggt gtacaccttg
     4201 atcttgtcgt gcgttttgcc gccaagcagg cgccataccg ggacgttgag caccttgccc
     4261 aagatgtccc aaagagccat ttcaatgccg ctgacggcgg tattgccaat cacgccaagg
     4321 cgccagaagg aatgcttgaa catgatgcgt acggcctgct cgatgtcgcg cggatcgcgg
     4381 ccaaccaaca acggctccag gtcctgaacc gcgccgacca cgccgcgtgt cttccactcg
     4441 agcgtggcct cgccccatcc caccagcccg ggctggtcgg tttctacgcg cacgaatacc
     4501 cagttgcgca tctctgcatt gcaaacagca gtcttgatcg ccgttatttt cattgttctc
     4561 ctccaaagac gtgagattaa agccaagact cgcgtgaagc cagtgcgacg gttcgggcat
     4621 aatcggcagg cattgcaata tttcggagat ccagttggcc caagcgatgg gaccattcga
     4681 ttacatcatt gtcggcgccg ggtccgccgg ctgcctgctt gcgaaccggc tgagcgcaga
     4741 tgcgaacatc cgcgtgttgc tgctggaagc cggtggcaaa gatgactatt tctggatcca
     4801 cgtgcccgta ggctatctct acaccatcaa taatccgcgc accgactggt gctaccggac
     4861 gcagcccgac gagggcctga acggccgcag tctcaattac gcgcgtggtc tcggactcgg
     4921 cggctgctcc tcgatcaacg ccatgatcta catgcgcggt cagaagagcg attacgacta
     4981 ctgggcagcg cttggcaacc gcgggtggtc ctgggatgaa gtcctgcctt atttcaagcg
     5041 caccgaagac tatgcccatg gtgccgatga tctgcatggc gcaggcggcg aattgcgggt
     5101 cgaggaacgc cgcgtgcact gggaaatcct ggatgcatgg cgcgacgcgg ccgaagaatg
     5161 cggcataccg aagatcaagg agttcaaccg cggggacaac ttcggcaacg cgtacttcca
     5221 gatgaaccag aagcgaggcg tgcgctggag caataccaaa gcatttctga gacccgtgct
     5281 gcaccggcca aatctcacag tgctcacaca cgcgcatgtc aaccggatcc ggttcgacac
     5341 atcggctggg cacaggcgcg caaccggcat cgaatttcgc acgcgcgacg gcgcaaacca
     5401 gtttgcgcaa gcgagacggg aagtcatcct ggccgccggg gcgatcggct cgccgcagat
     5461 tctgcagctt tccggcgtcg ggccgggcgc agtgctgcag aaatgcggcg ttcccgtgat
     5521 tcacgaactg cccggcgtcg gcgaaaacct gcatgaccat ctgcagatca ggatcatgta
     5581 caaggtcagc aacaccgtca cgttgaacga acgcacgcat agcctgttcg gaaaacttgc
     5641 catggggctc gagtaccttt tctatcgcac agggccgctg accatgccac cgagccagct
     5701 gggcgcattc gcgaa
//
LOCUS       DATMZQ010000203         8698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3954811, whole genome shotgun sequence.
ACCESSION   DATMZQ010000203 DATMZQ010000000
VERSION     DATMZQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3954811"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VN496_06475"
     CDS             complement(<1..358)
                     /locus_tag="VN496_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF16630.1"
                     /translation="MDALANNLQVLFQGLALGSIYALVALGFVLIIRATNVVNFAQGD
                     FAMLGAYIMVAFLIGLGMPYWLSFALALIGMGVFGLIFNYGVYFPLRNRGFLPVIIST
                     LGASIFLQNTVLAIFGA"
     gene            complement(425..1555)
                     /locus_tag="VN496_06480"
     CDS             complement(425..1555)
                     /locus_tag="VN496_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF16631.1"
                     /translation="MRVSLWSALSAGAVATLFALSAAAESIQIGLISEITGPNAEAGS
                     YTLNGAKLAVDTLNKKGGILGKQIELVIEDNQSTNPGTVLAFSKLGGNKEIPAIIGPI
                     RSTQIQAASPSIQKAGIPTMIGGTDPSLTHVNNPWVFRCRPNDTFSSRVIADFGVNKL
                     KLKKWAIVHSTDAFGSGGQKALTEVLKSHGVEPVLIQGYTNNSQDFTPVVLAIKKSGA
                     DVIGTYMTNSQDLGIFAKQLRQLGVAATWIGSPSIVTDTAMKLAGDSLHGTYGIADFN
                     PDANAEAKVFATAYRAKYNLEPDVYSSWAYDALTVIATAMNTAKSAKPDDIRKAILAL
                     KNWKGVEGNYTFDENGDGLRGYNIVKNEGGKVKFVEHIDVAK"
     gene            complement(1743..4301)
                     /gene="glgP"
                     /locus_tag="VN496_06485"
     CDS             complement(1743..4301)
                     /gene="glgP"
                     /locus_tag="VN496_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXF16632.1"
                     /translation="MASGTPFTLEVNPRLPLRLARLEELANNLLYSWDRQTRELFSRL
                     DPQLWEQVSHNPKAFLKRIDEKKLVAAADDPIFLGNFNRVLSAFDVYLKEPLRRNGSE
                     WLRKNDLVAYFCAEFGFHESLPIYSGGLGILAGDHCKSASDMRLPFVCIGLLYRQGYF
                     HQTIDTEGNQQALYADSDFEDLPVTPVRREGGSDVRIAIEFPGRNVQVKVWQALIGHV
                     TLYLLDTDLPENSADDRNITYRLYGGDRVTRIQQEIVLGIGGVRALAELGIAPTAWHI
                     NEGHPAFLVLERARTLVASGLPFAAALEAVAANTVFTTHTPVPAGHDHFSAETIGQYF
                     PWLRKQLGLTEEQFFALGLVDGIHEFNMTALATRGSRFQNGVSRIHGSVSSRICAPLW
                     PELESKENPLSYVTNGVHVPTFLAPEWSDQFDKFLGLDWANHAGDQDFRERLGSIPDH
                     LFWSVRQSLKAQMLHVVRHRVAQQHFRNRGSEAHLDRLLRYADPVNPNVLTIGFARRF
                     ATYKRATLLFRDLDWLRTILGDQTRPVLFLFAGKAHPADVPGQDVIREINRIGRLPEF
                     EGKVLLLEDYDLHLARRLVSGVDVWLNNPMYPQEASGTSGMKAGINGVLNLSVLDGWW
                     GEGYDGYNGWAIKPVAENLDETRRNLEESRTLYELLQDHVVPTYYRRGEMGYSADWIR
                     MAKQSIASILPRFSSTRMVNEYLARFYLPATRQGRRFAESDFEPARRLALWKQHVREV
                     WPKVTMRRLDATRKRIAFGEGLRLQVGVSLAGLAPEDISLELLLVKQAGDEANGRPST
                     YRFESEGVTTERGEQLFALELKPELCGKLEYRIRLFPQHELLTHPLELGMMRWL"
     gene            complement(4378..5709)
                     /gene="hslU"
                     /locus_tag="VN496_06490"
     CDS             complement(4378..5709)
                     /gene="hslU"
                     /locus_tag="VN496_06490"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HXF16633.1"
                     /translation="MSSMTPQEIVHELDKHIVGQDHAKRAVAIALRNRWRRQQVAEPL
                     RQEITPKNILMIGPTGVGKTEIARRLARLAEAPFIKVEATKFTEVGYVGKDVDSIVRD
                     LVETAIKQTREQETRKVRHRAEDLAEERILDTLLPTARETGTQNEAHDGDNATRQKFR
                     KRLRERELDDKEIEIELMAAQAQMEILAPPGMEDLTSQIQGMFQNLGSARRKMRKLKI
                     REAMNLLTEEEAAKLVNDDDLKLKALNNVEQNGIVFIDEIDKITSRQETGGAEVSRHG
                     VQRDLLPLVEGTTVNTKYGMIKTDHILFIGSGAFHLSKPSDLIPELQGRFPIRVELNS
                     LSVEDFERILTSTDACLTRQYQALLETEQVMLEFRADGIRRLAEIAWQVNEKTENIGA
                     RRLYTVMEKLLEELSFDAAREQGKDIVIDAGYVDVRLKELAQSEDLARYVL"
     gene            complement(5783..6322)
                     /gene="hslV"
                     /locus_tag="VN496_06495"
     CDS             complement(5783..6322)
                     /gene="hslV"
                     /locus_tag="VN496_06495"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXF16634.1"
                     /translation="MSEPFHGTTIISVRRGQKVALGGDGQVTFGNVVLKASARKVRRL
                     FHERILAGFAGGTADAFTLFERFEAKLEKHQGHLLRSAVELAKDWRTDRILRRLEAML
                     AVADHEASLIITGNGDVVEPELGLVAIGSGGPYAQAAARALLENTELQPQDIVKKALT
                     IAGEICIYTNQNHVIETLD"
     gene            complement(6429..7193)
                     /locus_tag="VN496_06500"
     CDS             complement(6429..7193)
                     /locus_tag="VN496_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXF16635.1"
                     /translation="MAHLEFSLLLPALIAGLLVTATHAPLGIQVLNRGIVFIDIAIAQ
                     IAGVGVIAADFFGWEGQGIAVQLAALSAALLGALFLTWTEKRWPDIQEAIIGTVFVLA
                     ATLEILLLAGNPHGGEYLKDMLVGQILWVGQMQVIVVAVLTAVVLGAWFGVGRQRLGR
                     VGFYLLFGLSVTASVQMVGVYLVFSSLIVPALASRNAVRWRLAKSYVVGGLGYLLGLV
                     ASNLFDLPTGAIIVWAMAVVGVIFSSLERTVPKPAV"
     gene            complement(7195..8109)
                     /locus_tag="VN496_06505"
     CDS             complement(7195..8109)
                     /locus_tag="VN496_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HXF16636.1"
                     /translation="MNSLNRWLSIAVTAALLLACTPSFAKVNVFACLPEWAALASEVG
                     GDKVTVYQASNALQDPHRIEARPSLVARMRSADLSICTGAELEIGWLPVLQQTAGNQK
                     VQPGQPGYIAAADFVDRLEVPTRLDRAEGDIHPAGNPHIQLDPHNVAKVGLVLTQRLE
                     QIDAANVPYYQARSKDFQARWNQAIARWEKDAAPLRGMRIVPYHKDTVYLIKWLGLVE
                     VMNVEPKPGIPPSAGHLSDLVARLKGGAADVISRSAYQDPKAVISLGEKTNLPVVELP
                     YTVGGTPGAKDLFGLFDDSIARLKAVKK"
     gene            complement(8140..>8698)
                     /locus_tag="VN496_06510"
     CDS             complement(8140..>8698)
                     /locus_tag="VN496_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16637.1"
                     /translation="RATKAQDRTYDDVDAIGTPVTNAFTGDSNMWGLDLVWKWAPNGN
                     TVDRNFKFQAEYMHRTEDGSLAFNTTGANLSDGYRSSQSGWYMQGVYQFMPRWRAGLR
                     YEELNSGNADIGLVNSGALTAADFPRLAQNDPKRSTAMIDFSPSEFSRFRLQFARDQA
                     RFNETDNQVFLQYIMSLGTHGAHKF"
BASE COUNT         1766 a         2562 c         2486 g         1884 t
ORIGIN      
        1 gcgccccgaa aatcgccagc acggtgttct gcaggaagat tgatgctccc agcgtggaaa
       61 tgatgaccgg caggaagccg cgattgcgca acggaaagta cacgccgtaa ttgaaaatca
      121 agccgaacac gcccatgccg atcagggcca gcgcgaaaga cagccagtac ggcatgccga
      181 ggccgattag gaaggcgacc atgatgtagg cgcccagcat ggcgaaatcg ccttgtgcga
      241 aattcaccac gttggtcgcc cggatgatca gcacaaaacc caacgcaacg agcgcgtaga
      301 tgctgccgag cgcaaggccc tggaacaata cctgcaaatt gttggctagc gcgtccacaa
      361 cgcatcgtct cccttgttca tccgtcctgt gccgatgcag acagggacgt gccgccggaa
      421 cgttttattt tgcgacgtcg atgtgctcga cgaacttgac cttgccgcct tcgttcttga
      481 cgatgttata gccgcgcagg ccatcgccat tttcgtcgaa ggtatagttg ccttcgacgc
      541 ccttccagtt cttcaatgca aggatcgcct tgcgaatgtc atcgggtttg gcgctcttcg
      601 ccgtattcat tgcagtggcg atgacagtaa gcgcatcgta ggcccagctc gaatagacat
      661 cgggttccag attgtacttg gcgcggtacg cagtcgcgaa aactttggct tcggcattcg
      721 catccggatt gaaatcggct atgccataag tgccgtgaag cgagtcgccg gccagcttca
      781 tcgcggtgtc cgtgacaatc gatggcgagc cgatccaggt ggcggccacg ccaagctgac
      841 gcagctgctt ggcgaatatg ccgagatcct gcgaattcgt catataggtg ccaatcacgt
      901 cggcgcctga cttcttgatg gccagcacca ctggcgtgaa atcctgcgaa ttgttggtat
      961 agccctgaat caataccggt tctacgccat gcgatttcaa tacctcggtc agcgctttct
     1021 gtcctcccga gccgaacgca tcggtcgagt gcacaatcgc ccatttctta agcttgagct
     1081 tatttacgcc gaaatcggca atcacgcggg aggaaaatgt atcgttcggg cggcacctga
     1141 aaacccatgg attgttcacg tgcgttaggc tgggatcggt tccgccgatc atggtcggga
     1201 tgccggcttt ctggatggat ggggatgccg cctggatctg cgtgctgcgg atcggtccga
     1261 tgattgccgg gatttccttg ttgccgccca attttgaaaa agccagcacc gtgccgggat
     1321 tggtggactg gttatcttcg atcaccagtt cgatctgctt gcccagaatc ccgccctttt
     1381 tgttgagcgt atcaacggcg agcttcgcgc cattcagggt ataactgccg gcttcggcgt
     1441 ttgggcccgt gatttcgctg atcagtccga tttggatcga ctcggcggct gccgacaagg
     1501 cgaacagcgt tgcaacagcg ccagcagaca gagcgctcca gagcgataca cgcatgaatt
     1561 tctcccaatg ttttttgtgc ttcgtagcgc atccaaccct ttgtgcacca gacctggcca
     1621 tgccgtccag ttgggccggg catgggctga ttgcatagag cgggcaggca tcgataggac
     1681 cgccgacggg cgttatgcta tgtctcgcct tactcagtgt caacgctgag ccagcgcgtt
     1741 tgctagagcc agcgcatcat gccgagttct agcgggtgcg tgagcaattc atgctgcgga
     1801 aagagacgga tgcgatattc cagcttcccg cataactcgg gtttgagttc cagagcaaag
     1861 agctgctcgc cgcgttcggt cgtcacgcct tccgattcga accggtatgt actgggccgg
     1921 ccgtttgcct cgtcgccggc ctgcttgacc aatagcaatt ccagcgagat atcttccggc
     1981 gccagaccag ccagagagac gccgacctgg aggcgcagtc cttcaccgaa cgcgatgcgc
     2041 ttgcgcgtcg cgtcgagccg tcgcatcgtg actttgggcc agacttcgcg cacgtgttgt
     2101 ttccacagtg cgagccgacg cgccggttca aaatccgatt cggcaaaacg gcgtccctgg
     2161 cgggtcgccg gcagatagaa cctcgcgagg tactcgttga ccatacgcgt cgatgaaaac
     2221 cgcggcaata tcgatgcaat cgactgcttc gccattcgaa tccagtctgc cgagtacccc
     2281 atctcgccgc gccggtagta ggtaggcacc acgtgatcct gcagcaactc gtacagcgtc
     2341 cggctctctt ccagattgcg gcgcgtctcg tccaggtttt cggccaccgg cttgatggcc
     2401 cagccgttgt aaccgtcgta cccctcgccc caccatccgt cgagcacgga aagattcagc
     2461 acgccattga tgccggcctt catgccggag gtaccgctgg cttcctgcgg atacatcgga
     2521 ttgttcagcc atacgtccac gccagagacc agccggcgtg ccagatgcag gtcatagtcc
     2581 tccagcagca aaactttgcc ttcgaattct ggcaggcgtc caatgcgatt gatctcgcgg
     2641 attacgtcct gacccgggac atccgccggg tgagcctttc cagcgaacag aaacagcacc
     2701 ggcctggtct ggtccccgag aatggtgcgc agccaatcga gatcccggaa cagcagcgtc
     2761 gcgcgcttgt aggtcgcgaa ccggcgcgca aatcctatcg ttaatacgtt cgggttgacc
     2821 ggatcggcgt aacgcagcag ccggtccaga tgggcttccg acccgcgatt gcggaaatgc
     2881 tgttgcgcca cgcgatggcg cactacgtgc aacatctgcg ccttgagcga ttgccgcaca
     2941 ctccaaaaca ggtggtcagg gatggagccc aggcgttccc tgaaatcctg gtcgccggca
     3001 tgattcgccc agtcgagacc cagaaattta tcaaactggt cggaccactc cggcgcgagg
     3061 aaagtcggca catgcacgcc gttggtgacg taggacaacg ggttttcctt cgattcgagt
     3121 tcaggccata acggggcaca aatgcgcgat gacacgctgc cgtggatacg gcttacaccg
     3181 ttctggaaac gcgaaccgcg ggttgcgagc gccgtcatgt tgaactcgtg tatcccgtca
     3241 accaatccca gcgcaaaaaa ctgttcttcc gtaaggccaa gttgtttacg taaccaggga
     3301 aaatactggc cgatggtctc ggcactgaaa tggtcatgtc ctgcgggaac cggggtatgc
     3361 gtggtgaata ccgtattcgc tgcgaccgct tccagtgcgg ccgcgaatgg aaggcccgag
     3421 gccaccaacg tacgcgcgcg ctccaaaacc aggaatgcgg ggtgaccttc attgatatgc
     3481 caggcggtag gagcaatgcc caattccgcg agcgcccgta ccccgccgat gccaagtacg
     3541 atttcctgct ggatacgtgt aacgcgatcg cctccataca agcggtacgt gatgttgcga
     3601 tcgtctgcag aattctctgg cagatcggta tcgagcagat aaagcgtcac atgtccgatg
     3661 agtgcctgcc atactttgac ttgcacgttg cgccccggaa attcgatcgc aatacgcaca
     3721 tccgaaccgc cttcgcgccg caccggggtg accggcagat cctcgaaatc cgagtcggca
     3781 tagagcgcct gctggttacc ctcggtgtcg attgtctggt ggaagtagcc ttgccggtac
     3841 agcaggccga tacagacaaa tggcagccgc atgtcgcttg ccgatttgca atgatcgccg
     3901 gcgagaatgc ccagtcctcc ggagtagatc ggcaggcttt cgtggaatcc aaactccgcg
     3961 cagaagtagg cgaccaggtc gtttttgcgc agccattccg aaccgttgcg acgcagcggc
     4021 tccttcaggt agacgtcgaa ggcggacagc accctgttga agttccccag aaaaatcggg
     4081 tcatctgctg ctgcgacgag ttttttctcg tcgatacgct tgagaaacgc ttttggattg
     4141 tggctgactt gttcccagag ctgcggatcg agccgcgaaa acagctcccg tgtttggcgg
     4201 tcccagctgt acaacagatt gttggcgagc tcttcaagcc gcgcgagccg gagcggaagc
     4261 ctgggattga cttccagagt gaacggggtg ccactggcca tagagggtct caggatgcca
     4321 aattatcaaa aagagttttg catataccgc acagcatgtc gccttagcgg gtgctcatca
     4381 caacacatag cgggcgagat cctcgctctg cgctagctcc ttgagccgga cgtcaacata
     4441 gcctgcatcg ataacgatgt cctttccttg ctcgcgcgcc gcgtcgaaag agagttcctc
     4501 gagcagtttt tccatgacgg tgtagagcct gcgtgcgccg atattttcgg ttttttcgtt
     4561 gacttgccac gcaatttccg ccaagcggcg gatgccgtcc gcgcgaaatt caagcatgac
     4621 ttgctcggtt tccagcagag cctggtattg gcgcgtaagg caggcatcgg tggacgtcag
     4681 gatgcgttca aaatcttcca cggacaggct gttcaattcc acgcggatcg gaaagcgacc
     4741 ctgcaattcc ggaatcaaat cggacggctt ggacaaatga aacgcgccgc tgccgatgaa
     4801 cagaatgtgg tcagtcttga tcatgccgta cttcgtattg actgtcgtcc cttctaccag
     4861 gggaagcaga tcgcgctgga cgccgtgccg ggacacctcc gcgccgccgg tctcctggcg
     4921 gctggtgatc ttgtcgattt cgtcgatgaa gacgatgccg ttctgctcca cgttgttcag
     4981 cgctttgagt ttgagatcgt catcgttcac cagctttgcc gcttcttcct cggtcagcag
     5041 gttcatcgct tcccggatct tgagcttgcg cattttgcgt cgcgcgctgc cgaggttctg
     5101 gaacatgccc tggatctgcg acgtcaggtc ttccatgccg ggcggagcca gaatttccat
     5161 ctgcgcctgt gcggccatca gctcgatttc aatttccttg tcatcgagct ctcgttcccg
     5221 caggcgtttg cgaaattttt gccgggtcgc attatcgccg tcatgcgcct cattttgcgt
     5281 accggtttcc cgcgccgtcg gcagcaaggt atccaaaatg cgttcttcgg caagatcctc
     5341 cgcgcgatgg cgcactttgc gcgtttcctg ttcacgggtc tgctttatcg cggtttcgac
     5401 caggtcgcgc acgattgaat cgacatcctt gccgacgtag cctacttcgg tgaatttggt
     5461 tgcctcgacc ttaatgaacg gcgcttccgc aagtctcgcc agtcgccggg caatttctgt
     5521 tttgcccact ccggtgggcc cgatcatcag aatattcttg ggcgtgattt cctgacgcag
     5581 cggttcagcc acctgctggc ggcgccagcg attgcgcaat gcaattgcca cagcgcgctt
     5641 ggcatggtcc tggccgacga tatgtttgtc cagttcgtgg acaatttcct ggggagtcat
     5701 ggaagacata aaaatttgct ggcggtgaaa ttgcgctgat ccggtgtggc agtcacgtta
     5761 gcgggacgca accgccaaac actcagtcga gggtctcgat cacgtgattc tggttggtgt
     5821 aaatgcagat ctcgccggcg atcgtcaacg cttttttgac aatgtcttgc ggctgcaatt
     5881 cggtgttctc caatagggca cgtgcagccg cctgcgcata aggaccgccg ctgccgattg
     5941 cgaccaggcc cagttcgggc tccacgacgt cgccgttgcc ggtgatgatc aatgacgcct
     6001 cgtgatcggc tactgccagc atggcctcca gccggcgcag gatgcggtcg gtacgccagt
     6061 ctttcgccag ttccaccgcc gagcgcagca gatgaccctg gtgcttttcg agttttgcct
     6121 caaagcgctc aaagagcgta aatgcatccg ccgtcccacc ggcaaagccg gcgaggatgc
     6181 gttcatgaaa cagccgcctg acttttcgcg cgctcgcctt cagtacgaca ttgccgaacg
     6241 tcacttggcc gtcaccgccc agtgcaactt tttgaccgcg gcgaacggaa atgatggtag
     6301 tgccgtgaaa gggttcgctc atcgagtgtt tttggaacta gcgcctggat gccgcagcct
     6361 gccttaagac aatgccggcg cccgaagcgg gcgccggcag gaatcagaaa ggaattgtac
     6421 tggagagttc aaacagccgg tttgggaaca gtgcgctcca ggcttgaaaa aatgacgccg
     6481 acgaccgcca tcgcccagac aatgatggcg ccagtcggca ggtcgaacag gttggacgca
     6541 accagtccga gcaggtaacc gaggccaccc acaacatagg actttgcaag ccgccagcga
     6601 accgcgttgc gcgatgcgag tgcgggaacg atcagactgg aaaataccag gtacaccccg
     6661 accatttgca ccgacgcggt gaccgagagc ccaaatagca gatagaagcc gacgcgcccg
     6721 agccgctggc gccccacacc gaaccacgca cccagcacaa cggcagtcag gaccgcaacc
     6781 actatcactt gcatttgtcc gacccaaaga atctgcccga ccagcatatc cttcagatat
     6841 tcgccgccgt gcggattccc ggcgagcaac agaatctcga gtgtggcggc gagcacgaat
     6901 acggtgccaa taatcgcttc ctgaatatcc ggccagcgct tctccgtcca cgtcaggaat
     6961 agcgctccga gcagcgcagc cgacagtgcg gccagctgta cagcgatgcc ctggccttcc
     7021 cagccgaaga aatccgctgc tataaccccc actcccgcga tctgcgcgat cgcaatatcg
     7081 atgaatacga taccccggtt gagtacctgg atccccagcg gggcgtgcgt cgcggtcacc
     7141 agcaaaccgg caatcagcgc cggaagcagt aagctgaact cgagatgagc catgttattt
     7201 cttaaccgct ttgagccgag cgatggaatc atcgaacagg ccgaacaaat ctttcgcccc
     7261 cggggttccg cccacggtgt acggtaactc gactaccgga agattggttt tttcgccaag
     7321 cgagatgacc gctttcgggt cctgatatgc agagcgggaa atgacatcgg ccgcgccgcc
     7381 tttcaagcgt gctaccagat cggaaagatg cccggcgctg ggcggaatgc ccggcttggg
     7441 ttcgacattc atgacttcca ccaggcccag ccatttgatg agatataccg tgtccttgtg
     7501 gtaggggaca atccgcattc cccgcaatgg agcggcatct ttttcccagc gcgcgatcgc
     7561 ctggttccaa cgcgcctgga aatctttact gcgcgcctgg taatagggga cattggctgc
     7621 atcgatctgc tccaggcgtt gcgtgagcac cagtccgact ttagcaacgt tatgcggatc
     7681 gagctggatg tgcggattgc cagccgggtg aatgtcgcct tcggcgcgat ccaggcgcgt
     7741 ggggacttcc agccggtcaa cgaaatcggc cgctgcgata taccccggtt gacctggttg
     7801 tactttctga tttcccgcgg tctgctgcag taccggcaac cagccgatct ccaactccgc
     7861 gccggtgcaa atcgacagat ctgcgctgcg catgcgcgcg acaaggctcg ggcgcgcctc
     7921 gatgcggtgt gggtcctgca aggcattgga cgcctgataa acggtgacct tatcgcctcc
     7981 gacttcgctg gcaagcgcag cccattcggg aaggcaggcg aatacattga ccttggcaaa
     8041 cgagggcgtg caggccagta gtagcgcggc cgttacggct attgataacc atctgttaag
     8101 gctgttcatg gttcttttcc ctttttgtga tgtcagtgct caaaacttgt gcgcgccgtg
     8161 ggtgccaagg ctcatgatgt actgcaggaa aacctggttg tccgtttcat tgaacctggc
     8221 ctggtcacgg gcgaactgca ggcgaaagcg ggagaactcg gacggtgaaa aatcaatcat
     8281 tgccgtgctg cgtttgggat cgttttgcgc caatcgcggg aagtccgcgg cggtgagcgc
     8341 gccgctgttg accagcccga tatcggcatt accgctgttc agttcttcgt agcgcagccc
     8401 cgcgcgccag cgcggcatga attgatatac cccttgcata taccagcccg actggcttga
     8461 tcggtatcca tccgacagat ttgcgcctgt agtgttgaac gccagcgacc cgtcttcggt
     8521 gcgatgcatg tattcggcct ggaacttgaa gttgcgatcg accgtgttgc cgttcggcgc
     8581 ccacttccac accagatcca gcccccacat gttgctgtcc ccggtaaagg cattggtaac
     8641 tggggtaccg atagcatcca catcgtcgta ggtgcggtct tgtgccttgg tggcgcga
//
LOCUS       DATMZQ010000204        11067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3262541, whole genome shotgun sequence.
ACCESSION   DATMZQ010000204 DATMZQ010000000
VERSION     DATMZQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3262541"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VN496_06515"
     CDS             <1..820
                     /locus_tag="VN496_06515"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXF16638.1"
                     /translation="HRAGIEQAFNQYSVLVFPDQHLTTDQHLDFARNFGPLETTIHAT
                     RSDAKLRVRAEIADVSNLSPDEQIWDRASRKRMFEMGNRMWHTDSSFRRVPAKASLLY
                     ARSIPPVGGQTEFADMRAAYDALPSEAKERLSGLVAEHAIMYSRKKLGFTDFSDEENQ
                     NLPPVPQTLVRRHAGSGRVGLYLASHAGRIFGMPDDEGTALLQQLIAHATQRQFVYAH
                     RWRVHDLVMWDNRCTMHRGKGYDDLRWPRDMQRATVSDIAPTCEQEGIAVPAAA"
     gene            1173..2090
                     /locus_tag="VN496_06520"
     CDS             1173..2090
                     /locus_tag="VN496_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HXF16639.1"
                     /translation="MTNFLFSARNWLAAATAIFVALLSFALFAQDAEAARLGGGRSFG
                     RQSQGVTRQQAAPPQQPAKGAATNPAQQQAPAPAGNRWLGALGGIAAGLGIAALLSHF
                     GLMGPFASALGSILVIGLLIFAGFLLWRLFSARSNPAVAGRSRNAMFNEPPAPVMQGS
                     TASTNVYGAPVRQMPESASSWNVPATFDTAAFLRSAKVYFTRLQAAWDARDFEDIRRF
                     TTPEAFAEIRMQGNEALGKTDRTEIEQLDASLLGIETTPTEYVASVRFTGALREDGGA
                     PEAFEEVWNLAKPTDGRTGWMLAGIQQVH"
     gene            2372..3325
                     /locus_tag="VN496_06525"
     CDS             2372..3325
                     /locus_tag="VN496_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF16640.1"
                     /translation="MKTRKLGRSGLEVSPFAFGGNVFGWTIDEPTSMRLLDRFVAEGF
                     NFIDTADMYSRWVAGHRGGESETIIGKWLKKTGKRDSVVIATKVGMEMGADKKGLAPD
                     YIERAVENSLERLQTDYIDLYQSHTDDAETPLADTLGAYEKLIRAGKVRAIGASNYSA
                     ERFDEALALSKKHSLPRYETIQPLYNLYDRAEFEQKLQGVCIANAVGVIPYFSLASGF
                     LTGKYRSEADFGKSPRGQGMKKYLNDRGFRILAALDKVARETAATPARVALAWLMARP
                     GVTAPIVSATNLPQLEELFAATRLILDSSARAQLDAASMAA"
     gene            complement(3404..3703)
                     /locus_tag="VN496_06530"
     CDS             complement(3404..3703)
                     /locus_tag="VN496_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXF16641.1"
                     /translation="MKANILLFLRTNGEQLDAEIAKALHISKPQVLTHIAELSAAGDV
                     ICCQVTRFNGGKKIEGVSCRLSAYVPPKATGPKPGAKRAAAANANAHNHNEVNIA"
     gene            complement(3929..4537)
                     /locus_tag="VN496_06535"
     CDS             complement(3929..4537)
                     /locus_tag="VN496_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16642.1"
                     /translation="MKLKTAIAVLVVGICPAVVWAGADKDSSQQPQASDQHNSSAGGA
                     SAESAQSNGKTTGWQYVDGRAASAERGVQNTQQPQGSSDSANAANAGQATNAESAESA
                     ESNSSNDQDEHQSAAVESGSEQQMGGDQSTQLQGIPEGMEDKLVVILPTNWQGSLRDL
                     LAALEQTSQASEILVLRREGQDDDMSSEDSSDVEDTSDTSSR"
     gene            complement(4719..5348)
                     /gene="msrA"
                     /locus_tag="VN496_06540"
     CDS             complement(4719..5348)
                     /gene="msrA"
                     /locus_tag="VN496_06540"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXF16643.1"
                     /translation="MDRFFRFAFFVLIFVPFPFAASAQTKTTNAAAAQPDAVATAIFA
                     GGCFWCVEADFDKVSGVLSTESGYAGGQLKNPTYEQVSRGGTGHAESVRVTYDPQKVS
                     YEQLLDYFWHHVDPTVKDQQFCDKGDQYRTVIFYQNDAQRVAAQASKAALEKSGRLSH
                     VYTELVPAGTFYPAEEYHQDYYKKNPIRYKFYRTSCGRDARIVEVWGHD"
     gene            complement(5494..6462)
                     /locus_tag="VN496_06545"
     CDS             complement(5494..6462)
                     /locus_tag="VN496_06545"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HXF16644.1"
                     /translation="MGKRASAKKHGRTNGASQWQRLLATVGILLAYAFAHCAHAGNDV
                     QTTFDSTEAAVDALVEAVKVNDVTMLGSILGPRTRKLLDSGDEVADAHGREAFLREYS
                     EANKIVTEDDGRATLLVGTDEWPLPIPLVKFGERWHFDSAEGEKEIIARRIGTNELSA
                     IQICVAIVEAQNDYAGQVAAVDGVPHYAARFASSPGKHDGLYWQSNADESPSPLGPLL
                     AAAAQEGYTSAEVKPLAPYHGYYYKILTRQGDNAPGGAYDYFVRGKLIGGFAVLAYPA
                     RYGASGVMTFMFNHDGVVYEKNLGRKTQDVAAGISRFDPDQTWHKQ"
     gene            complement(6440..7981)
                     /locus_tag="VN496_06550"
     CDS             complement(6440..7981)
                     /locus_tag="VN496_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HXF16645.1"
                     /translation="MRASPRVAATQLVALQLCASVAFAQTTDSQVIELGPADSADAQS
                     GQAATDQPSTQTAFTQPELDQMLAPIALYPDALLSQILMASTYPLEVVQAARWSKANP
                     GMSGDHAVKAAEQNNWDPSVKSLVAFPQIVAMMDEKLDWTQRLGDAFLSQQQQVMDTV
                     QELRRKAHAAGNLESNQNVRVEPQGQTIVIEQANPQVVYVPYYDPTYVYGSWWYPAYP
                     PVYWGPWPGYYPYPGFGAGFFWGAGIAIGAGFFFGHCDWHHHHVTVVNNFYHRPVARH
                     SAGNWMHDPGHRWGVPYRDAGLRREFSHADARPDARRDFRGNPPQRLQGPGTVQDIRP
                     DMRSAPSSRSQTGDRVRPRQDGNSRPAAPDLRSGPRAAATPTAPRRMPRADDMPTASS
                     RMPRADAMSTAPSRMPRADAMPTAPNRMPRADVMPTPTSRVPRADTMPTAPSRPVVQS
                     RPHVFDGIGQGPVVRDSSQRGNSSYQRAGQPQPVAQPQRSSAPRQGGGQRSSGDGQHG
                     QARQR"
     gene            complement(8203..9171)
                     /locus_tag="VN496_06555"
     CDS             complement(8203..9171)
                     /locus_tag="VN496_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXF16646.1"
                     /translation="MARKASQLPALDFFRGFEAAARHLSFTRAAEELFLTQSAISRQI
                     HALEERLGAKLFTRRNRGLALTEAGREMYRAADIALRTLHEAAERIAPGNTQDMVTVT
                     SSMAFCSLWLIPRLSAFRNTRPDLDVRISANNQVLDLEREQIDVAIRYCPVMAAPPGA
                     IRMFGEEIVVVCSPQLLKDRARPLRSPGDLRRHTLLHFDHAEGSIPWLSWSVWLESAR
                     MPELKSAGSLRFSHYDQVIGAALRGQGIALGRRPLVADLLADGALVAPLRQDSVTDRA
                     YFIVRSAAVRSRRDVDDFIAWLIAESAALVRIMDKHKRNRRHARPR"
     gene            9303..9608
                     /locus_tag="VN496_06560"
     CDS             9303..9608
                     /locus_tag="VN496_06560"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HXF16647.1"
                     /translation="MQIELNSSTLNLSPAGVIAVQDGTGTQVLCLSGVLWVTEEGESK
                     DSIVRAGDVLTLRKPGRTVISALEAASLTLMGPEPYKLNGSRQRDVHRLVADTVACS"
     gene            9599..10144
                     /locus_tag="VN496_06565"
     CDS             9599..10144
                     /locus_tag="VN496_06565"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXF16648.1"
                     /translation="MQLTALPNHVSPRAPKTHAIRSVRPNDEIGLQQFIGRLSPGSRH
                     ARFMMAIRELPDYLLDRFIHPVRGREAVLVATSPRGGIIGLGQYVRDEIGDGCEVAVV
                     VEDSWQRQGLGTDLLEAVLLVARESGIRHFHAEALADNYPMRALARKIGCKIRINREA
                     AFLVRISGIVQSPATISASMN"
     gene            complement(10167..10826)
                     /locus_tag="VN496_06570"
     CDS             complement(10167..10826)
                     /locus_tag="VN496_06570"
                     /inference="COORDINATES: protein motif:HMM:NF042805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HXF16649.1"
                     /translation="MATSNANLSGEELAQAAAESVKSGEHIRERVRDLTLRAFQTRRF
                     DYEGMKEVLDAMTTGISLGAGQRAGKLRGVVADALSGLDQALMKSAEASQLALQELAA
                     KHREFSDSELKQAIDQMKRLESDFIGAAGKVAESGSAAVRAEFRDFVTHAQRFGTDTG
                     TVVAQTMREFSERIALQMTDAQIAGLEAARQLNTRFSQAASGFLQAMADSLREEKKPK
                     H"
BASE COUNT         2262 a         3296 c         3160 g         2349 t
ORIGIN      
        1 acatcgcgct ggcatcgaac aggcgttcaa tcagtactcg gtgctcgtat tcccggacca
       61 gcatcttacg actgaccagc acctcgattt cgcgcgcaat ttcggcccgc tcgaaacgac
      121 tatccacgct acgcgcagcg acgcaaagct gcgcgtgcgt gccgagattg cggatgtgtc
      181 caatctgagc ccggacgaac agatctggga cagggcgagc cgcaagcgca tgtttgaaat
      241 gggcaaccgg atgtggcaca cagacagttc gttccggcgc gtgcccgcca aggcgtcgct
      301 gctgtacgcc cgcagcattc cgcccgtcgg aggccagacg gaatttgccg atatgagagc
      361 cgcctacgac gctttgccaa gtgaagcaaa ggagcgactg agcggactgg tggccgagca
      421 tgccatcatg tactcgcgaa agaaattggg ctttaccgac ttttccgacg aagagaacca
      481 aaatcttccg cccgttccac aaacgctcgt gcgtcgccat gccggatccg gacgcgtggg
      541 actttacctt gcctcgcatg ccggccgcat cttcggcatg ccggacgatg aaggtactgc
      601 cctcctgcag caactgattg cgcatgcgac ccaacgacag ttcgtctatg cgcatcgctg
      661 gcgtgtgcat gatctggtga tgtgggacaa ccgctgcacc atgcatcggg gtaaaggtta
      721 cgacgatttg cgctggccgc gagacatgca gcgcgccacg gtctccgata ttgcgccgac
      781 ctgtgagcag gaaggcatcg ccgtgcccgc ggcagcataa gatcggttct tcgtcaccac
      841 cacccgcgcg acagcatcgc gcaaggtggc cgggctggtt ccgcgcatgc ttgctattcc
      901 gtctgacatt tcgggttcgc gggttcgaat cacacccagc cgacctggca cactcggcac
      961 cgacagcgtg atgtccatgt cttctgcaag atagcaaata ccgcagcaaa atcgcgagtg
     1021 tgctgcgcat tgttcgtccc gaggcaaaca gtacggcgta cactgccgcc acgggttggt
     1081 ccgcgatgcg ccataatgca tgttctgcct atcttgaaac ccgatcacca cccccaatat
     1141 caattgcttg aatcaacctt gatcctatgg cgatgacaaa ctttcttttt tcagcgcgaa
     1201 actggcttgc cgctgcaacg gcaatttttg ttgccctgct gagttttgca ctgttcgcgc
     1261 aggacgccga agccgcacgg ctgggcggcg gcaggagttt tggtcgccag tcgcagggcg
     1321 taacccgaca gcaggcggca ccaccgcagc aaccagcaaa aggtgctgcg actaacccgg
     1381 cgcagcagca agcacctgcg cccgcaggca accgctggtt gggcgccctg ggtggcatcg
     1441 cggctggatt gggcattgca gccctgcttt ctcacttcgg gttgatggga ccgttcgctt
     1501 cggcgctcgg cagcatccta gtgatcggat tgctgatctt tgccggcttt ctgctatggc
     1561 ggctgttctc ggcgcgcagc aaccccgccg ttgcaggacg cagccgcaat gcaatgttca
     1621 atgaaccacc cgcgccggtc atgcaaggct cgaccgcatc gaccaatgta tatggtgcac
     1681 cggtgcggca aatgccggag tcggcatcat cctggaatgt gccggcgacg ttcgataccg
     1741 cggctttttt gcgcagcgcc aaggtttatt tcacgcgact gcaggcagcc tgggacgcca
     1801 gggactttga agacattcgc aggtttacca ccccggaagc ctttgctgaa atcaggatgc
     1861 agggcaatga ggctctgggc aagaccgatc gaactgaaat cgaacagctt gatgcaagcc
     1921 tgctgggcat cgagaccacg cccacggaat acgtagcaag cgtgagattc accggagcgc
     1981 tacgcgaaga cggcggcgcg ccggaagcat tcgaagaggt atggaatctg gccaagccaa
     2041 ccgacggccg aaccggctgg atgttggcgg gaatccagca ggtgcattag cggcgcccac
     2101 aatagttcgc gccggtgaag agggcacact agagggtgcc ctttttttgt gcgcgatttt
     2161 cggttgtgcg cttcttcaaa atcctgaccg gctgcagcgc gatagaattc agccgtcata
     2221 agtacacata ggcttgcggc acacgcgacg ctgcaagcgc gcaccaccgg tttggtggat
     2281 catgacctgt tgcactgcta gcgtgctgct cacgtccacc ccaagcctgg acgctggcaa
     2341 tcggtcgcct ctttaatgca aaggaacatt catgaaaact cgcaagctgg gccgctccgg
     2401 tctggaagtt tctccgttcg cattcggggg taatgtattc ggatggacca tcgacgaacc
     2461 gacctccatg cgcctgctgg atcgtttcgt ggccgaaggc ttcaatttta tcgataccgc
     2521 tgacatgtat tcacgctggg tggcaggcca ccgtggcggc gaatcggaga ccattatcgg
     2581 caaatggcta aagaaaaccg gcaagcggga cagcgtcgtc attgcaacca aagtgggcat
     2641 ggaaatgggg gcggacaaga aagggcttgc gccggactat attgagcgag cagtagagaa
     2701 ttcgctcgaa cggctgcaga ccgactatat cgacctgtat caatcacaca ccgacgatgc
     2761 ggaaacgccg cttgccgaca cgctgggcgc ttacgaaaaa ctcatcagag ccggcaaggt
     2821 cagagcgatc ggcgcgtcaa attacagcgc tgagcgattt gatgaagcac tggcactgag
     2881 caagaaacac agccttccgc gctacgagac aatccagccg ctctacaacc tttacgaccg
     2941 tgccgagttc gagcagaagt tgcagggcgt ctgcatcgca aatgctgtag gcgtgatccc
     3001 ctatttctct cttgcaagcg gttttctgac cggaaaatac cgatccgagg cggatttcgg
     3061 gaagagtccg cgcggacagg ggatgaaaaa atatttgaat gaccgcggct tccggattct
     3121 ggcagcgctg gacaaggtag ctcgtgaaac ggcggcaacg cccgcgcgcg tggcattggc
     3181 gtggctaatg gcgcggcccg gcgtaacggc gccgattgtc agcgcaacca acttgcccca
     3241 actcgaggaa ctcttcgcgg cgacacgcct cattcttgat tcatcagcac gcgcacaact
     3301 cgatgccgca agtatggcgg cctgaagcgc cccgcaaaaa cacacgcccc ggtaacgggg
     3361 cgtttaactg gaaatgcaac ggtgtattgc ccggtgcgtt acgtcacgcg atattcactt
     3421 cgttatgatt atgggcgttc gcgttggcgg ctgcggcacg cttggctccg ggtttgggtc
     3481 cagtagcctt cggcggtacg taagcagaca gacggcaact cacgccctca atcttcttcc
     3541 ctccattgaa gcgcgtcact tggcagcaaa tcacgtctcc ggcggccgat agttcagcaa
     3601 tatgtgtcaa aacctgcggt ttcgagatat gaagggcctt ggcaatctcg gcatccaatt
     3661 gttcgccatt ggttctcaaa aataaaagaa tgtttgcttt catgcgaggg attcctgcgg
     3721 ggtggcttcg agtgggtcgc acagtttgcg cttttgccgg cttctgagca agaccaaatc
     3781 cacgcgttat tcagaaataa ctgagcaatc tgcaccgaat cccttgattg caggttggtt
     3841 atacactgta gcgagtgccc gctgggcttc ccggatatga agcccttcag gattcgcacg
     3901 ccagccgagc gctggcgcca ttcatctctt atcgtgaact cgtgtcggag gtgtcttcaa
     3961 cgtcgctgga gtcctcggac gacatgtcat cgtcctgacc ttcccgcctg agcaccagga
     4021 tttcggaagc ctgtgaggtt tgctccaggg cggccagcaa atcgcgtagt gacccttgcc
     4081 agttcgtggg gagaatgact acaagcttgt cttccatacc ttccggtatg ccttgcagct
     4141 gagtcgattg gtcgccaccc atttgctgtt ccgacccgga ttcgaccgcc gccgattggt
     4201 gttcgtcctg atcattactg ctattgctct cagcactttc tgcgctttcc gcattcgttg
     4261 cctgccctgc gtttgctgca tttgcagagt cagaactacc ttggggctgc tgcgtatttt
     4321 gcactccgcg ctcagcactc gcggctcgtc catcgacata ctgccatccg gtggtttttc
     4381 cgttgctctg tgccgactcc gcggatgcgc cgccagccga ggagttatgc tgatcggatg
     4441 cttgcggctg ttgcgatgaa tccttgtctg cgcccgccca aaccactgcc gggcatattc
     4501 ccaccactag tacagcaatt gcagtcttaa gcttcatgtc atctcctgtt cagttgcgcg
     4561 cgggactgat gcgtaccgca attcgccgga aaaaatttct ccctgtaaaa gaggacatgc
     4621 cgcatccaat caggttccgg aattgctcaa ttcgaaatgt aacggcggag taggccgttt
     4681 accgacatgg actacagcac ttggccggtt gctgcagctt agtcgtgtcc ccacacctca
     4741 acgatccgcg catcgcgacc acaggacgtt cggtagaact tgtagcgtat ggggttcttc
     4801 ttgtaatagt cctggtgata ttcttcggcg ggataaaaag tgccggcagg gacgagttcg
     4861 gtatacacat gcgacagccg tccgctcttc tcgagcgcag ccttgctcgc ctgcgctgca
     4921 acgcgctggg catcattctg ataaaaaatt accgtgcggt actggtcgcc tttatcgcag
     4981 aactgctggt ccttcaccgt ggggtccacg tgatgccaga aataatccag taactgttca
     5041 tagctgactt tctgcgggtc gtaggttacg cgcacggatt ccgcatgtcc ggtaccgccg
     5101 cggctgacct gttcgtacgt tgggtttttc agttgtcccc cggcatagcc ggactcggtg
     5161 ctcaatacgc cagaaacctt gtcgaaatcg gcttcaacgc accagaaaca gccgccagca
     5221 aaaatcgccg tggccaccgc gtccggctgt gcagcagcgg cgttggtggt cttagtctgt
     5281 gcggatgcgg cgaacggaaa cggtacgaag atcaatacga aaaacgcgaa acgaaagaag
     5341 cggtccatgt ttttttcctg gtaagcgatt cattgacgta cagccggcaa gttgccgctg
     5401 tctttgagca atacggtcgt tagcccggcg tggattcacc ttccgccatc tcgtagtcaa
     5461 tgaaaccgcg cagcagcgcg cggcgacggg cgctcactgt ttgtgccagg tctggtcggg
     5521 atcgaagcgc gatatcccgg cggcgacatc ctgtgtcttg cgacccagat ttttttcata
     5581 aaccacgccg tcgtgattga acataaaggt catgacaccg gatgcgccgt accgtgccgg
     5641 ataagcaagg actgcaaaac cgccgatcaa ctttccacgc acgaaataat cgtacgcacc
     5701 cccgggcgcg ttatctccct ggcgggtcag aatcttgtag tagtaaccat gatagggcgc
     5761 aagcggctta acttctgcgc tcgtgtaccc ctcctgcgcg gccgccgcaa gtagcgggcc
     5821 gagcgggctc ggcgattcat cggcattgct ttgccagtaa agtccatcgt gcttgccagg
     5881 actgctggcg aaacgcgccg catagtgcgg aactccgtcc acagccgcaa cctgacccgc
     5941 atagtcgttc tgggcctcta cgattgcaac gcagatctga atagccgaaa gttcgttggt
     6001 accgatgcgg cgggcaatga tttctttttc gccttccgca ctatcgaagt gccagcgctc
     6061 gccgaatttc accaacggaa tcggcagcgg ccattcgtcc gtgcccacca gcagggttgc
     6121 gcgaccatcg tcctcggtca caatcttgtt ggcttcacta tattcgcgca ggaacgcttc
     6181 gcgtccatgc gcgtccgcta cctcatcgcc tgaatcaagc agcttgcggg tgcgcggtcc
     6241 gaggatcgaa ccaagcatgg taacgtcatt gaccttcacc gcttcgacga gggcatcgac
     6301 ggccgcctca gttgagtcga acgttgtctg cacgtcattc ccggcatgcg cgcaatgcgc
     6361 aaaggcgtaa gccaataaaa tgccgaccgt tgccaatagt ctctgccatt ggcttgcgcc
     6421 gttggtcctt ccgtgctttt tagcgctggc gcgcttgccc atgctgaccg tccccggaag
     6481 atcgctgccc gccaccttgc cgcggggcgg aggagcgttg cggttgcgct accggttgcg
     6541 gctgtcctgc tcgctggtag ctgctgttgc cgcgctgact ggaatcgcgc accaccggcc
     6601 cctggccaat gccatcgaac acgtgtggcc ttgattgcac gaccgggcgg ctgggtgcag
     6661 taggcatggt gtctgcacgt ggtactcggc tcgttggggt aggcatgaca tccgcacgcg
     6721 gcatgcggtt tggcgcggta ggcatggcgt ctgcacgcgg catgcggctt ggcgcagtag
     6781 acatggcgtc tgcacgcggc atgcggcttg acgcagtagg catgtcgtct gcacgcggca
     6841 tgcggcgcgg tgccgtcggc gtagcggcgg cacgcggacc gctgcgcagg tctggcgctg
     6901 ccggccgcga attcccatct tgacgcggcc gtacgcgatc accggtctgc gaccggctac
     6961 tcggagcact gcgcatatca ggtcgaatgt cctgaaccgt tcctggtcct tgcaatcgct
     7021 ggggcggatt gccgcggaaa tcccttcgcg catccggcct cgcatccgca tgcgaaaatt
     7081 cccggcgcaa tcccgcatca cgataaggca cgccccaacg gtgacccgga tcgtgcatcc
     7141 agttgcccgc ggaatggcgc gcaaccggcc gatggtagaa attattgacg acagtgacat
     7201 ggtgatggtg ccaatcgcag tggccaaaga aaaaacctgc ccctatcgca atgcctgcgc
     7261 cccagaaaaa tccggcaccg aatcccggat acggatagta gcctggccac ggcccccagt
     7321 agacgggcgg ataggccggg taccaccagg atccatagac atatgtcggg tcgtagtacg
     7381 gcacgtagac gacctgcgga ttggcctgct cgatgacaat cgtctggccc tgcggctcaa
     7441 cacgcacatt ctggttcgat tccagattcc ccgcagcgtg tgccttacgc cgcagttcct
     7501 gtacggtatc catcacctgc tgctgctgcg aaagaaaggc gtcacccagc cgctgcgtcc
     7561 aatccagctt ttcatccatc atggcaacga tctgcggaaa ggcgaccaat gacttgacgc
     7621 tgggatccca attgttctgc tctgccgcct ttacggcgtg atctccgctc attcccggat
     7681 ttgcttttga ccagcgcgcg gcttgcacga cttcaagcgg ataagttgat gccatcagaa
     7741 tctgcgacaa caatgcgtct ggataaagtg cgatcggcgc gagcatctgg tcgagttccg
     7801 gctgcgtgaa tgctgtctgt gtcgagggtt ggtccgtcgc agcctgcccg gattgcgcat
     7861 cggcggaatc cgccggtccc agctcgatta cctgcgagtc tgtggtctgc gcaaacgcga
     7921 ccgatgcgca gagctgtagt gcgaccaact gtgtggctgc gacccttggg cttgctctca
     7981 tgggttcact ccttgttttg gtcagtcccg ggcactgccc ggtaccgcac tatccggccg
     8041 gaaaaaccgg gtctgtaccg ctagtaacgc tgaagattcg acaaaagcat acacccacga
     8101 gttgcacgtt taccttacgc gtgacagttc gtgacaagaa agtgaaacgg cgcaccatgg
     8161 accgtctcca gcacattgat aactatgacg gtgtgcggat tgctacctcg gtctggcgtg
     8221 tcttctgttc cttttatgct tgtccatgat gcgtaccaaa gccgcgcttt cagcgatcag
     8281 ccaggcaatg aaatcatcca catcgcggcg agaacgcacg gccgcagaac gcacgatgaa
     8341 atacgcacga tctgtgaccg aatcctggcg caacggcgca acaagtgcgc catcggcgag
     8401 aagatctgcc acaagcggac ggcggccaag cgcgattccc tgaccacgca gcgcggcgcc
     8461 gatgacctga tcataatgac tgaagcgcaa tgatcccgcc gatttgagtt ccggcattct
     8521 tgccgactcc agccagacac tccacgagag ccacggtatg gagccctcgg cgtggtcgaa
     8581 atgcagcagc gtgtgacgac gcagatcgcc cggtgacctc agcggccgcg cgcgatcctt
     8641 cagcaactgc ggactgcata cgacaacgat ttcctctccg aacatgcgga tggcaccggg
     8701 cggcgccgcc ataacggggc agtaacgaat cgccacatca atctgctccc gctccagatc
     8761 gagcacctgg ttgtttgccg aaatgcgcac gtccagatcg ggccgcgtgt tacgaaaggc
     8821 agacaggcgc gggatcagcc acaacgaaca aaaagccatt gatgaagtta cagtgaccat
     8881 gtcttgcgtg ttccccggag caatccgctc agcggcttca tgcaaggtgc gcagagcgat
     8941 gtccgccgcg cgatacatct cccgtcccgc ctcggtcaga gccagccccc ggttacggcg
     9001 cgtgaacagt tttgcgccaa gccgctcttc cagtgcatgg atctggcggc tgatggcgga
     9061 ttgcgtcagg aacaattctt ccgccgccct ggtgaaactc aggtggcgag ccgccgcctc
     9121 gaagccccga aaaaagtcga gtgccggcaa ctgactggct ttcctagcca tgcgtgtagc
     9181 tcatgcgaaa cattccaaaa gatcgtttgg tgcgctctta cagagtgcgg aaaatagcat
     9241 cgtgcaatga tttactggaa tggccggcag gtcaaccgca cggtcatgtg atcggaggca
     9301 acatgcaaat cgaattgaat tcttccacgc ttaacctttc gccggccggc gtcattgccg
     9361 tgcaggatgg cacaggcacg caggtgctgt gcctatccgg cgtcctctgg gtcacggagg
     9421 aaggagaatc gaaagattcc atcgtaaggg caggcgacgt gctgacgctg cgcaagccgg
     9481 gccggacggt gattagcgcg ctcgaagcag cctcgttgac gctgatgggg cccgaaccat
     9541 acaagcttaa cgggtcgcgg caaagagacg tgcatcgact ggttgcggat accgtcgcat
     9601 gcagctaact gcgctgccaa accacgtttc tccccgcgcg ccaaaaacgc acgcgatccg
     9661 gtcggttcgt cccaacgatg aaattggcct gcagcagttc atcggccgcc tgtcaccggg
     9721 cagccgccat gcgagattta tgatggcgat tcgagaactc cccgattacc tcctcgaccg
     9781 gttcatccat ccagtgcgcg gaagagaagc ggtattggtg gcaacttctc cgcgtggcgg
     9841 gattattggg ctcggacaat atgtgagaga tgagattggg gacggctgtg aagtagcagt
     9901 cgtcgtggag gattcgtggc aacggcaagg gctgggaacg gaccttcttg aggccgtgtt
     9961 gctcgtcgca cgagaaagcg gtatcaggca tttccatgcc gaggcgcttg cagataacta
    10021 tccgatgcga gcgctggctc gcaaaattgg atgcaaaata cgaatcaatc gtgaagcggc
    10081 cttcctggta cggatctcag ggatcgttca atcgccggcc acgatcagcg cttcaatgaa
    10141 ttgacaaaca ggaccgttcc gaaggcctag tgttttggct ttttttcttc ccgtagtgaa
    10201 tcagccatgg cttgcaggaa tccgcttgca gcctgagaaa atcgtgtatt cagctgccgc
    10261 gcggcttcca gaccggcgat ttgcgcatcc gtcatttgta aggcaatgcg ctcggaaaac
    10321 tcacgcatcg tctgcgccac caccgtgccc gtatcggtgc caaaccgctg cgcatgcgtc
    10381 acgaagtcgc gaaactccgc cctgaccgcc gcgcttccgg actcggccac tttgccggcc
    10441 gcgccgatga aatcgctctc cagccttttc atttgatcaa ttgcctgctt caattcgctg
    10501 tcgctgaact ccctgtgctt cgcggcgagc tcttgcaagg caagttgtga ggcttccgca
    10561 gatttcatca gtgcctggtc cagcccggac aacgcatctg caacaactcc tctgagtttg
    10621 cccgcccgct gaccggctcc caaacttatg cctgtcgtca tggcgtccag cacctctttc
    10681 ataccttcat aatcgaagcg ccgggtctgg aaagcgcgca acgtgaggtc gcgaacacgt
    10741 tcacggatat gctcgccgct tttgaccgat tccgccgccg cttgcgcgag ctcttcgccc
    10801 gataggttag cgttgctggt tgccatcgct gcctccattg gaatttagga aaggttatgt
    10861 gaaattggct ccctgttgtg cgtctgccct ggccgccgcc tcgaagcatg tctggaagac
    10921 cgtttgccta caccgtactt tcaacgcacg atgatttcaa cccgccgatt gcgaagctct
    10981 gccacagcct cggatgactt gaccaccggt tcacgcttgc cccgaccggc agcctggatc
    11041 atcttggcag ggataccgcg cttgatg
//
LOCUS       DATMZQ010000205        20362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_157443, whole genome shotgun sequence.
ACCESSION   DATMZQ010000205 DATMZQ010000000
VERSION     DATMZQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157443"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VN496_06575"
     CDS             <1..320
                     /locus_tag="VN496_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXF16650.1"
                     /translation="YLAPILSIRIVNDLDHAIEHISTYGSQHTDAIVTEDITRARRFL
                     REVDSASVMVNASTRFADGFEFGLGAEIGISTDKLHARGPVGLEGLTSLKFIVLGDGH
                     IRE"
     gene            372..2156
                     /gene="lpdA"
                     /locus_tag="VN496_06580"
                     /pseudo
     CDS             372..2156
                     /gene="lpdA"
                     /locus_tag="VN496_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
     gene            2159..3031
                     /gene="nudC"
                     /locus_tag="VN496_06585"
     CDS             2159..3031
                     /gene="nudC"
                     /locus_tag="VN496_06585"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HXF16651.1"
                     /translation="MVLASPDRFTALITPPTQPTGPALWFAFQGAQILVMRDTQSASV
                     PNALDVRELGVSPKSSLYLGVLDGQHCFACELEEGSAAPEGMQWSGLRALFGSIDDAL
                     FALAGRAFQIVDWDRSHLFCGRCGTPTQIKNHERARECPNCGQVHYPRIAPAIMALVR
                     RGREFLLARSPHFAPGMYSALAGFVEPGETLEQTLVREVREEVGIEVTNVRYFASQPW
                     PFPHSLMIAFNADYAGGEITPDPGEIEAADWFTPERLPQALPSKISISRRLIDATLAE
                     LGADKVDKSGRRHA"
     gene            3024..3644
                     /locus_tag="VN496_06590"
     CDS             3024..3644
                     /locus_tag="VN496_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXF16652.1"
                     /translation="MLRIWGRSNSVNVQKALWCCEEMNLPYERVDAGGSFGVVNTQQY
                     RHLNPNGLVPTIEEDGFVLWESNAIVRYLAARHSPGKLWPEDLNVRAEADKWMDWQNT
                     TFWPTFRPLFWNLVRTPPDQRDAGAMEESRLKTAEILGYLDAHLKNRAYIVGDALTVG
                     DIPLGCAIWRWLGLPIERSDLPNVQRWFEALGGRPAYKKIVMLPLT"
     gene            3730..4617
                     /locus_tag="VN496_06595"
     CDS             3730..4617
                     /locus_tag="VN496_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF16653.1"
                     /translation="MHPIARVLTGLLAALLFACPNVFAQSRFATLGHVDLSFYEVTAK
                     VVQGVLERLGYRVEVKSGSHAEIYPLLGKGDVDLFVAVWLPNAHATYWEQYKDTSVKV
                     TTLFEDARLYWSVPDYVPASDVSSVADLRKPEVAAKMQKEIRGTRADSGLMIGSRKIM
                     EEYALADAGYQLIPGKPADWIANFEGNIAARRWFVMPLWQPQYLNRVAKMRILEEPKQ
                     LLGGVDKAYLVAHKDFRDKVDKHTWNALQRMSLSLKAVTELDYLVNVKKLTPEYAARN
                     WMAAHPDTVNFWLQPDSDE"
     gene            complement(4640..5275)
                     /locus_tag="VN496_06600"
     CDS             complement(4640..5275)
                     /locus_tag="VN496_06600"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HXF16654.1"
                     /translation="MRNRTALCLMTAAGLSALCIIAAAGAESYSGRVVIELLDEIEFA
                     HHFRLLEDFTFVDARGKPWVARKGGIADDEAVPRNLSALYGLPYVGEYRKAALLHGYF
                     CRTMTEPWQQVHRVLFDASLVEGLTETQAKLLYAVVYAGSWRWEMQASSCYRSCHASS
                     ASLVWKPAATAAEIEPVLQWISHDAPDLDAIDRRVDQVVKRPGPHLFAQRP"
     gene            5592..6935
                     /locus_tag="VN496_06605"
     CDS             5592..6935
                     /locus_tag="VN496_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF16655.1"
                     /translation="MEFNVLQHFKGLAELGKDLGTDPFDGDQVGTSLFMTGGMGPQIS
                     KKMDESAKKAGMSRRNFIRSSLGFSAAMLAANEVTGMKFFEVNAAQAADIDAKNEAIQ
                     IARASGDFIVDVHTHVCTRPGHYQLGVNTTERGMWFVQLLDDLGKAFGLPNGTRDMNV
                     ENYGKLILQESDTTVGIFNPFGFREDYGGQDMIPMEYQVGVRDRWPKSTLMLAGGLTP
                     NQGLSVTLDRLTQYVEQYKISGLKLYTFDSTKKKGWWFDDEKLAYPIWEKCRKLGIKN
                     VGCHKGIPFGQFYARYAHAEDFDAAADDFLDLNFIAYHSAWPYHAELAALKGFKPQRK
                     NLYCEVGSTFAATVTSRPLECAHVLGTLLRDLGPDYVLWGTDSLLWGNPQWQIEAFRN
                     FQIPDQLVEGHGYPKITPEIRRKVFGENAARIWNIDKKAAMTAKAEILANKSSYA"
     gene            7017..7304
                     /locus_tag="VN496_06610"
     CDS             7017..7304
                     /locus_tag="VN496_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXF16656.1"
                     /translation="MPTYEYACDPCLTIYRTRHGMNETGPARCRTCNGELRKVISAPS
                     LNTRNHSSPTQAKYSKLSESDEIAKEKQLQKVYQTIWVPPEVKHSPWDHDH"
     gene            7330..8751
                     /locus_tag="VN496_06615"
     CDS             7330..8751
                     /locus_tag="VN496_06615"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXF16657.1"
                     /translation="MINVARRIVVLATFGLATWIATANEEAADPAAMTAGPFTTPESG
                     EQAFSIPYIGLDKDQGRQFADGHAEFNEAWVLAPARGVWGLGPTFNEDRCAHCHVNNG
                     RAPAPDEGGEVQQGMLVRLSIPGKTKEGGPLPHQSYGDQLQNRGIENRVPAEGQAVIR
                     YREKEIVFADGEKKMLRSPKIEMQNLQFGDLGPETMMSVRVAQQLVGLGLLEAVPESA
                     MLATAKSQQKTAFAGRPNYVWDIENERKVIGRFGWKANQPTLRQQTAAAFNGDIGATS
                     FLFPEKNCPPIQTQCVELPSASKCGGQGGCTGNAFRPEVSPSRLTNITFYLQTLTVPA
                     RRSAGDPEVKRGESIFTALQCGVCHVPELQTGPKAFIPAAANVTIHPYTDLLLHDMGD
                     ELADGRPDFEASGREWRTAPLWGVGLIRTVNGKLFLLHDGRARDVAEAILWHGGQAQA
                     ASEAFISLSKAERDALVKFVESL"
     gene            complement(8792..9973)
                     /locus_tag="VN496_06620"
     CDS             complement(8792..9973)
                     /locus_tag="VN496_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXF16658.1"
                     /translation="MRAKPLSGIRILDLTRLLPGPVCTLHLADLGADVIKIEDTGAGD
                     YARTLGALPGKLAPVFAAINRNKRALRLDLKQHEGVQVFLKLVRDADAVVEGFRPGVV
                     DRLGVGYAACCAVNPRIVYCAISGYGQNGPYRERAGHDVNYCSYAGFIDQNGSEQGNP
                     VLPNMQIADILGGAVVPAMGILAALLDAQRSGKGRYVDVAMTEGVLVHNLQVLAAVAT
                     NGKSPPRGGDFLSGREPCYAVYRTADGRHMAVGALERKFWDRVCDVLQRPDLKACHWE
                     IRALSGGRDAHWGKAQTSAIFAAQSQAYWVKQFSGEDCCVSPVLTLEEIFEDPHIRAR
                     EMVVRQSGTLQFTPPWKISEYEFAVERPAPEAGEHTEEILRAAGYASEDISRLRDGGI
                     I"
     gene            complement(9980..10552)
                     /locus_tag="VN496_06625"
     CDS             complement(9980..10552)
                     /locus_tag="VN496_06625"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HXF16659.1"
                     /translation="MNTGLTTATPRKLMHRRHIECIGYHREDGLWDIEGHLVDTKSYD
                     LNDLDRGPAPSGQPLHEMWLRLTVNDELVVIEVEARTVWGPYHMCGDITPNFQVLKGL
                     KIQGGWTQKTRELLGGTKGCTHLLELLGPLATTAFQTIYPLRARRERAQVVRKRPGLI
                     DSCHAWAADSPVIAKRFPEFYTGPKKATAG"
     gene            complement(10549..10740)
                     /locus_tag="VN496_06630"
     CDS             complement(10549..10740)
                     /locus_tag="VN496_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HXF16660.1"
                     /translation="MNADANNISSPCVNVCQMNPFNGLCRGCLRTLDEIAGWLDFSAS
                     EKLAVLQRIEERRRTGTSR"
     gene            complement(10737..11654)
                     /locus_tag="VN496_06635"
     CDS             complement(10737..11654)
                     /locus_tag="VN496_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXF16661.1"
                     /translation="MRYPPQVKIVDVSPRDGLQNEKVSVPTQVRVELIDRLSDAGLQS
                     VEAGSFVSPKWVPQMADTSDVMARIRRRPGVAYPVLVPNMQGLESAIAAGATEVAGFA
                     SATEAFSMRNTNCTIVESMKRQKSVCDAARKAGLRVRGYISVVLDCPYEGEVKPQAVA
                     EVAIQLRDMGCYEISLGDTIGTGTPARAQKLIETLARRIPIDMLAGHFHDTYGQALAN
                     ILAVMELGVATFDSSAAGLGGCPYARGATGNVATEDVLYMLNGLDIKTGIDMNRLLDA
                     AQFICHHLGRPTASRAGRALSAKRDKAGA"
     gene            complement(11651..13675)
                     /locus_tag="VN496_06640"
     CDS             complement(11651..13675)
                     /locus_tag="VN496_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HXF16662.1"
                     /translation="MFDKILIANRGEIACRIARTARRLGVGTVAVYSHADAHAMHVSA
                     CDEAWMIGPAHPRDSYLRGDRILDVATRSGAKAIHPGYGFLSENAAFARACQQAGIVF
                     IGPTPEAIAAMGSKSAAKALMEKAGVPLVPGYHGPAQGLATLQEEAARIGYPVLIKAS
                     AGGGGKGMRAVKHPSEFAQALAAAKREAASSFGDDAVLIEKYLQRPRHIEMQVFADLH
                     ATCLHLFERDCSVQRRHQKILEEAPAPGMTEERRRQMGAAAVAAAKAVNYVGAGTVEF
                     IAEPDGRFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGEALPLRQEQLSIDGHA
                     IEARIYAEDAARDFIPVAGRIDHLRLPPPSAHVRVDSGIQEGDEIGVYYDPLLAKLIA
                     WDLDRPSALRRLRAALAQYQVTGVVTNISLLSAITAHSAFAHADRDPGALHTALIESN
                     RLALFPEHAPVPDTILAIAVLSETLHMDSEAAAAAAHSPDPYSPWHLRHGWRLNQGNH
                     HAFVFVDAGERKEIVAHYREGGYLLDLPGGRRLSASATPADDQSLNVELDGRRSPARV
                     VRRAGDITVFCDGRSYLLAFEKAAVIEEEDPSGRLVSPLPGSVIRVLVSSGETVVKGQ
                     ALMIIEAMKMEHTVTAPRRGTVKQIYFGEGQQVVEGVQLLEFEPEQVSKS"
     gene            complement(13715..14149)
                     /locus_tag="VN496_06645"
     CDS             complement(13715..14149)
                     /locus_tag="VN496_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16663.1"
                     /translation="MPVHMHPDAAVDAQRAKLLALFCALGLAMFLLDLGAGFWLWHYM
                     GQYKFTLPGFERLLGELRSAQGPAIADIALRTREGWAACEEARGGTIETLIHIAMAAS
                     FIGLALFSVCFALALLLHRSLSQLPGGRAEALAPDDVDDTWK"
     gene            complement(14153..14935)
                     /locus_tag="VN496_06650"
     CDS             complement(14153..14935)
                     /locus_tag="VN496_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXF16664.1"
                     /translation="MTQEILQTELAAGVATLRMNRPDVHNAFDDVLIAALTAELRRLD
                     RLPAVRVVVLAANGKSFSAGADLNWMRRMAKYSHEENLGDAMALAALMRTLDGMNKPT
                     IAKVQGPAFGGGVGLVACCDIAIASTQSSFSLSEVRLGLIPSVISPYVIAAIGERAAR
                     RYFMTAERFDALEARRIGLVHEVVAGAELDQMVASMAGHLLKGGPRALASAKELIADM
                     GRRPMDDKLAMETARRIAAIRVDSEGQEGIAAFVEKRIPDWK"
     gene            complement(14949..16100)
                     /locus_tag="VN496_06655"
     CDS             complement(14949..16100)
                     /locus_tag="VN496_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF16665.1"
                     /translation="MESIYLNEEHVALRDQVARFIDKEVEPSGKAWETQGCVPREVLR
                     KMGELGFFGITYPVEYGGSEADAMSALVVAEALSRSTFGGFVVTALVHSDMASPHLVN
                     AGSKAQLDRYLPGIIRGEAITAVAVTEPDAGSDVAGIRTRAVRDGNEWVLNGAKMFIT
                     NGVHANLYFVAARTDVQAKGSRGISMFIVEKGLPGFRVGRALDKTGWLCSDTAELVFE
                     DCRVPAENLLGEENAGFYAVMKNFQRERIAAGAMAVGNALTSLRLTIEYVKNRKAFGG
                     VLFDKQVIRQRLAMLEAKTHAARQFLYHCGWLAAQKRECVREVSMLKALTGELVNEVV
                     YTCQQFHGGMGYMRESAIERQARDARVLAIGGGATEVMLEEVAKRFGPD"
     gene            complement(16122..17729)
                     /locus_tag="VN496_06660"
     CDS             complement(16122..17729)
                     /locus_tag="VN496_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXF16666.1"
                     /translation="MSVIQSKVNPRSPEFKAGQEAMGAMVADLRAKIELIDQGGGEAA
                     REKHIGRGKLLPRERVRRLLDVGSPFLELSQLAAWNMYGNQVASAGILTGVGRIAGRE
                     CVVVANDATVKGGTYFPITVKKHLRAQEIAQQNHLPCIYLVDSGGANLPTWDEVFPDR
                     DHFGRIFYNQANMSAMGIAQIACVMGSCTAGGAYVPAMADESIIVRNQGTIFLAGPPL
                     VKAATGEIVSAEELGGADVHARVSGVADHYANDDAHALALTRRIVSNLNRKKEIALEL
                     REPRAPLYEAAELHGIVPADTRKSFDIRDVVARVVDASELDEFKALYGSTLVCGFAHI
                     HGYPVGIIANNGILYSESALKGAHFIELCAQRGVPLVFLQNITGFMVGKKYEAGGIAK
                     DGAKLVAAVSCARVPKFTVIVGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQA
                     ASVLAQVKRDGIEKSGKTWSREDEEAFKSPIRAQYEVQGHPYYASARLWDDGVIDPAD
                     TRMVLGLGLSAALNAPIERTRFGVFRM"
     gene            complement(17737..18372)
                     /locus_tag="VN496_06665"
     CDS             complement(17737..18372)
                     /locus_tag="VN496_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXF16667.1"
                     /translation="MAEYLLYGFAQSGNSYKPALYLELAGADWAPRFVDFFNGETRTP
                     AYRAINVMGEVPVLQHNKHDLSQSGVILDYLVEQFGKYGWNNNEERREILRWLLWDNH
                     KLTSYTATYRFLRVLMPNPNPVVVEEFRKRAEASWKILDAHLARNRYVVGGRMTIADI
                     SACGYLFFADELGVDWAQYPSINDWLQRIRSEPRWKHPYELLPGHPIPKRS"
     gene            complement(18377..18979)
                     /locus_tag="VN496_06670"
     CDS             complement(18377..18979)
                     /locus_tag="VN496_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXF16668.1"
                     /translation="MANPIQFYFDFASPYGYMAGTRITALAAKHGRTVDWKPILLGVV
                     FKVTGGVPLPNAPLKGDYSRRDMERCARLFNIPYKLPTKFPIAGQSPSRVIYSLEAEG
                     AERQGEVTLALYRAYMVDDRDISSPEITADVAASVGLNRQKTLDAIADPVMKEKLKVE
                     TEAAIASGVFGSPYIIVDGEPFWGFDRLEHVDRWLQNGGW"
     gene            complement(19005..19916)
                     /locus_tag="VN496_06675"
     CDS             complement(19005..19916)
                     /locus_tag="VN496_06675"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF16669.1"
                     /translation="MANKKIGFIGVGLMGHGIAKNLSEKGFPTTVLGHRNRVPVDDLV
                     KRGAKEAKDVAHLVKSSELIFLCVTGSAQVEDLVYGKAGILESVVNGQIVVDTSTSQP
                     GSTLKIAADLSSKGVRFADAPLTRTPVEAEQGRLNTMVGADQATFLEIEPALKSFCEN
                     IFHVGDVGAGHKIKLINNCAAIGQMALIAEALTACAKLGVDPKKYFALVSSGAANSGI
                     FQMMAGKAVEGDFGGMKFGLANALKDVRYYSQMAMEFGVAGSMAAATLQTLTQAVNLG
                     FGGPDHLVASLVPAVAKLNNTTFPPHA"
     gene            complement(19997..>20362)
                     /locus_tag="VN496_06680"
     CDS             complement(19997..>20362)
                     /locus_tag="VN496_06680"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486589.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXF16670.1"
                     /translation="ATVIGHATHAQESALFTTAPVGAMRKLFDSTGWTSDQVDLYEIN
                     EAFAVVTMAAMREHKLPHEKVNVHGGACALGHPIGASGARIVVTLIGALRKRGLRRGV
                     ASLCIGGGEATAMAIELIK"
BASE COUNT         4344 a         6068 c         5800 g         4150 t
ORIGIN      
        1 aatatctggc accgattctg tcgatacgca tcgtgaacga tctggaccac gcaatcgaac
       61 acatcagtac ttacgggtcg cagcacaccg acgccatcgt caccgaggac atcacgcgcg
      121 cgcgtcgttt tctgcgcgaa gtggattccg cttcggttat ggtcaatgcg tcaacacgtt
      181 tcgcggacgg tttcgaattc ggtctcggcg cagaaatcgg catttcaacg gacaagctgc
      241 atgccagggg cccggtcggg ctggaaggtc tcacgtcgct caagttcatc gtcctcggtg
      301 acggtcacat ccgcgaataa gcgttgcgcg tgcccgccag cggcgagcca agaacaacaa
      361 agaggagttc catgagcaat ctgatcgagg tcaaagtccc cgacatcgga gatttcaaga
      421 acattcccgt tattgaggta ctggtcaaac ccggcgacac aatcgcaaag gaagattcgc
      481 tgatcacgct gtagtcggac aaggcaacaa tggaagtgcc ttcgccggtc gccggagttg
      541 taagggaact caaggtcaaa acgggcgaca agttgtcgca aggcagtctg atcctgatgc
      601 tcgacagcgc cgcggcgagt gcttcagcgc cgaaggcgca agcgccggtt gagcaagcag
      661 acgcgcgaaa agccccaagt tcgcctggcg cagctgcggt aaagaatcct gcgtctgcgt
      721 ctgcatctgc cgcgccggga ccaagaggcg acattcatgc acaggtagtc gtattgggtt
      781 caggccccgg cggttacact gccgcgttcc gagcggctga tctcggcatg cagaccgtac
      841 tgattgaacg ctacccggtc ttgggtggcg tctgcctgaa tgtgggctgc atcccatcga
      901 aggctttgct gcatgcggcg cgcgtgattg ccgaggccga ggaaatgggg cactacggat
      961 tggcgttcgg caagccaaag atcgaactcg ataaattgcg cgaatggaaa acctcggtgg
     1021 tagaaaaatc cattcgtgga ctcgcgggtc tggcgaagca gcgcaaagtc accgtcgtgc
     1081 aaggcgcggg gactttcacc tcgccacaga tgatggaggt tgcaaccgct gacggaataa
     1141 agaaagtatc gttcgagcac gccatcattg ccgcgggctc gcaatcggcc cggattccag
     1201 gctttcctta cgacgacccg cggctgatgg attcgacctc cgcactgacg ttgatcgatg
     1261 tgcccaagcg gctgctggtc attggaggtg gaatcattgg tctcgagatg gcttgcgttt
     1321 acgacgcact tggcgcaaag gttacggtag tggagttgct cgatggcttg attcccggtg
     1381 ccgaccgcga tctgatccgg ccattgcaaa aacgcatcga gaaacgctat gagggcattt
     1441 atctgaagac cagggttacg cagcttcagg cgcaacggga tggcttgaaa gccacgttcg
     1501 agggtgagaa cgcacccgcg ccccaagtgt acgaccgcgt gctgctggcg gtcggtcggc
     1561 gtccgaatgg caaggcgatt gcagcccagg cggctggcgt gcttgtcaac gaccgcggtt
     1621 tcattccggt ggacaagcaa ttgcgcacca acgtgccgca tatctttgcg atcggcgaca
     1681 tcgttggcga gcccatgctg gcacacaagg cgagctacga aggcaagatt gcggcggaaa
     1741 tcattgccgg gcacaaagcg gcgttcgacg cgcgcactat tccttcggtg gcctacacgg
     1801 atcccgagat ttcctggatg ggtttaacgg aaacgcaggc caaggcacaa ggcatcgaaa
     1861 tcgacaaggc gagctttccg tgggcagcca gcggtcgcgc gcgcgccatg gcacgggacg
     1921 aaggcatgac gaagctcatc ctcgacaaaa aaacacgccg cctgctgggt gctggcatcg
     1981 tcggccctaa cgccggcgaa ctgattgcgg aaacggtact ggcgcttgaa atgggcgcgg
     2041 acgcggccga tatcgcactc accattcatt cgcatccgac gctgtccgag accgtcatgt
     2101 tcgctgctga aattgccgaa ggcagcatca ccgacctgta catggcgaag aaataaccat
     2161 ggtgctggct tcgcccgacc ggttcaccgc gttgatcacg ccacctacgc aaccgaccgg
     2221 gcctgcactc tggtttgctt ttcagggcgc ccagatcctg gtgatgcgcg atacgcaaag
     2281 cgcgagtgtc cccaacgcac tggatgtgcg cgaacttggc gtatcgccga agagcagtct
     2341 ttatcttggc gtcctggatg gccagcattg tttcgcctgt gaacttgaag agggcagcgc
     2401 cgcgcctgaa ggcatgcaat ggtcggggtt gcgcgcactg ttcgggtcca tcgatgacgc
     2461 gctgttcgcg cttgcgggcc gcgcattcca gatcgtggac tgggaccgtt cgcatctgtt
     2521 ctgtgggcgc tgcgggacgc ccacacaaat caagaaccac gagcgggcac gcgaatgtcc
     2581 caattgcgga caggtgcact atccgcgtat cgcgccggca atcatggcgc tggtcaggcg
     2641 cgggcgggaa tttctgctgg cacgttctcc gcattttgcg cccggtatgt acagcgcgct
     2701 ggccggtttc gttgaaccag gagagacgct cgagcaaacg ctggtacgcg aggtccgcga
     2761 agaggtgggc atcgaagtga ctaatgtgcg ttactttgcc agccagccat ggccttttcc
     2821 gcactcgctg atgattgcgt tcaacgccga ttatgcgggt ggtgaaatca cgccggatcc
     2881 tggcgaaatc gaagcggcgg actggtttac gcccgaacgg ttgccgcaag cattgcccag
     2941 caagatcagc atctcgcggc gtctaattga cgctacgctg gccgagcttg gcgctgacaa
     3001 agtcgataaa tcagggaggc ggcatgctta ggatttgggg acgcagcaat tcagtgaacg
     3061 tacaaaaggc attgtggtgc tgtgaggaga tgaatctgcc gtacgagcgt gttgatgcag
     3121 gaggaagttt cggagtagtc aacacgcaac agtaccgcca tctgaatccc aatggcctcg
     3181 tgccgaccat cgaagaagac ggctttgtct tgtgggagtc gaacgcgatc gtgcgttacc
     3241 tggctgccag acacagccca ggcaagcttt ggccggagga tctcaatgtc cgcgctgagg
     3301 ccgacaagtg gatggattgg cagaacacaa cgttttggcc cacgtttcgg ccgctattct
     3361 ggaatctggt gcgtacgccg ccggaccagc gcgatgcagg ggccatggag gaatcccgcc
     3421 ttaagaccgc cgaaatactg ggttacctgg atgcgcatct gaaaaaccgt gcgtacattg
     3481 ttggcgatgc actgactgtc ggagacattc cgctgggttg tgcaatctgg aggtggctcg
     3541 gcttaccaat cgaacgcagc gatctgccca atgtgcagcg ctggttcgaa gcccttggcg
     3601 gccggccagc gtacaagaaa atcgtcatgc tgccgctcac ctgaggcagc gaggctcgaa
     3661 aaagtaaatg tcaggcattt ggcgagctta ctttcaaggt cgcccgactc acatgatccg
     3721 gacccattca tgcatccaat agcacgcgtc cttaccggcc tgctggccgc tcttcttttt
     3781 gcctgcccaa atgtctttgc gcaatcgcgt ttcgcgacgc ttgggcacgt ggatctgtcc
     3841 ttctatgaag tcaccgccaa agttgtgcag ggggtactgg agcggctggg ctatcgcgtc
     3901 gaggtaaaga gcggcagcca tgccgaaatt tatcctctgc tgggcaaggg ggacgtggac
     3961 ttgttcgtcg ccgtctggtt gccgaacgca cacgccacct actgggaaca atataaagat
     4021 acctcagtaa aagtgaccac gctgttcgag gacgccagac tttactggtc cgtgccagat
     4081 tacgtaccgg ccagtgatgt cagcagcgtg gcggatctgc gcaagcccga agtagcggca
     4141 aagatgcaga aggaaatccg cggcactcga gcggattcgg gcctcatgat tggctcccgg
     4201 aaaatcatgg aagaatatgc attggccgat gccggttatc agctgatacc gggcaaaccg
     4261 gccgactgga tcgctaattt cgaaggcaac atcgcagcca ggcgctggtt tgtcatgccg
     4321 ctctggcagc cgcaatatct caaccgggtt gcgaaaatgc gtatcctcga agaaccaaaa
     4381 cagcttttgg gtggcgtgga caaggcctat ctggtggcgc acaaggattt tcgcgacaag
     4441 gtcgacaagc acacatggaa cgcactacag cgcatgtccc tgtcgctgaa ggcagtaacg
     4501 gaattggatt atctggttaa cgtcaagaaa ttgacgccag aatatgccgc gcgtaactgg
     4561 atggcggcac atccggacac ggttaatttt tggctgcagc ctgattcgga tgaatgaact
     4621 gaaatcattc aaagccacat caaggccgct gcgcaaacaa atgcggcccc ggcctcttca
     4681 caacctggtc gacgcgccgg tcaatggcat ccagatccgg cgcgtcgtgc gagatccatt
     4741 gcaacacggg ttcgatctct gctgccgtgg cagccggttt ccacaccaaa gacgctgacg
     4801 aggcatggca tgaccggtag caactcgatg cctgcatttc ccaacgccag cttccagcat
     4861 aaacgaccgc ataaagtagc ttagcctgcg tttcggttag cccctctacg aggctggcat
     4921 caaacaggac ccggtgcact tgttgccaag gttccgtcat ggtgcggcag aaatagccgt
     4981 gcaaaagcgc ggcctttcgg tattcaccaa cgtacggaag cccataaagg gccgacaaat
     5041 ttcgtggtac ggcttcgtcg tcagcgatac cgcctttgcg cgcaacccaa ggtttgccgc
     5101 gagcatccac aaacgtgaaa tcctccagca agcgaaaatg gtgagcgaac tctatttcgt
     5161 ccaatagctc gataacgacg cggccagaat agctctccgc gcccgccgcc gcgatgatgc
     5221 acaaagccga gaggcccgcc gcagtcatca gacaaagcgc tgttctgttt ctcaaaacgc
     5281 tctcctccag cacgcgctca atacgcgtca gacgatacag caaattatct ggtgactgct
     5341 tgtctcgaga aacgcgatgc cggccgaatc gctaaccggc agccagagca ggatgcttgt
     5401 cctacagtgg aaacgatcac cgaaaacgcg aaatcgcgcc gcagcatcgg ttcactttga
     5461 cagaccctcc agccaagagt agttttttta gcggaataac acggttatca atttgaccgt
     5521 gttcgtggtc gggtttggtg gtgtggaaga tttctcgctg taaatataac caaatttggg
     5581 ggagaaacac catggaattt aatgtcttgc agcacttcaa aggtcttgcg gaactcggca
     5641 aggatttggg cactgatcca ttcgacggcg atcaagtcgg cacttcgctg ttcatgaccg
     5701 gcggcatggg cccgcagatc tcaaagaaaa tggatgaaag tgcaaaaaaa gcggggatgt
     5761 cacggcgcaa tttcattcga tcgtcactgg gattttcagc ggcgatgctg gccgcgaacg
     5821 aagtgaccgg gatgaaattc ttcgaggtga atgcagcgca ggctgccgac atcgatgcca
     5881 agaatgaagc gatccagatc gcgcgagcga gcggagattt cattgtcgat gtgcataccc
     5941 acgtctgcac acgtcctggc cattatcagc tgggtgtcaa taccaccgag cgcggcatgt
     6001 ggttcgtgca attgctcgac gatctcggca aggctttcgg acttccgaac ggcactcgcg
     6061 acatgaacgt ggagaactac ggcaagttga ttttgcagga aagcgatact acggttggca
     6121 tattcaaccc cttcggcttc cgcgaggact atggcggcca ggacatgatc cccatggagt
     6181 atcaagtggg cgtccgggac aggtggccca agtccacact gatgctggcc ggcggcttga
     6241 cgccgaatca gggattaagc gtgacgctgg atcgcctgac gcagtacgtg gagcaataca
     6301 agatctcggg cctgaaactc tatacattcg attccaccaa gaagaagggc tggtggttcg
     6361 acgacgagaa gctggcttat ccgatctggg aaaaatgccg caagctcggc atcaagaacg
     6421 tcggctgtca caaggggatt ccgtttggac agttctatgc ccgttatgcc catgccgaag
     6481 atttcgatgc agcggccgac gatttcctcg atttgaattt catcgcgtac cactccgcct
     6541 ggccttatca cgcggaactt gccgcgctga aaggattcaa gccacagcgc aagaacctgt
     6601 attgcgaagt tggatctact tttgccgcaa ccgtaacaag ccgccctctg gaatgcgctc
     6661 atgtactggg cacattgctg agagacctgg gaccagatta cgtgttgtgg ggcaccgact
     6721 cgctgctatg gggtaacccc cagtggcaaa tcgaggcatt ccgcaatttc cagatccctg
     6781 atcagctggt ggaaggccac ggatatccga agatcacccc ggaaatccgt cgcaaggtgt
     6841 ttggcgaaaa tgccgcgcgc atctggaaca ttgacaagaa agcggcgatg actgcaaagg
     6901 ccgaaatact ggctaacaag agcagctacg cctgactttg aagcaacaag catgaccggg
     6961 gccgattcct tcgggatcgg cccttttggt ttcaagcaga atgccggaga ttccaaatgc
     7021 caacctacga atacgcttgc gatccttgcc tgaccatcta tcgaacgcgc catggaatga
     7081 atgaaacggg tccagcgcgc tgcagaacct gcaatggcga actccggaaa gtcatatcgg
     7141 ctccgagtct taatacgcgc aatcactcca gcccgaccca ggcaaaatat tcgaagctgt
     7201 ccgagagtga cgagatcgcc aaagagaaac aactgcaaaa ggtctaccag acgatttggg
     7261 tacctcccga agtgaagcac agcccgtggg accacgatca ctgagcgcgg gacgccgaat
     7321 cggcttccca tgatcaatgt tgcccgcagg atcgtcgtgc tggccacatt cggccttgcg
     7381 acgtggattg ctacggccaa tgaggaagca gccgatccgg ccgcgatgac cgccggacca
     7441 ttcacgactc cggaatccgg tgagcaggcg ttttcgattc cctacattgg gctcgacaag
     7501 gatcaggggc gccagtttgc ggatgggcac gccgaattta acgaagcatg ggtacttgcg
     7561 cccgcgcgcg gcgtttgggg actggggccg accttcaacg aagatcgatg cgcgcattgc
     7621 cacgttaaca atggacgcgc gccagcgccc gacgaaggtg gagaagtaca gcaagggatg
     7681 ctggtcaggt tgagcatccc gggcaagaca aaggaaggcg ggccactgcc acaccagagc
     7741 tatggcgacc agctgcagaa ccgcggtatc gagaatcgcg tgcccgcaga aggccaggca
     7801 gtgatccgct accgcgaaaa ggaaattgtc tttgctgatg gcgagaaaaa aatgctgcgc
     7861 tcgccaaaaa tagagatgca gaatttgcag ttcggcgatc tcggcccgga gaccatgatg
     7921 tctgttcggg tagcgcagca gctggtgggc ctcggtttgc tcgaagccgt gccagagtcg
     7981 gcgatgctgg caacagcgaa gtctcagcag aagactgctt tcgccggtcg ccccaattac
     8041 gtctgggaca tcgaaaacga gcgcaaggtg attggcagat tcggctggaa agcgaatcag
     8101 ccgaccctgc gacagcaaac cgccgcggct ttcaacggag acatcggcgc aacttcgttt
     8161 ctgtttccgg aaaagaattg cccgccgatt caaacgcagt gcgtcgaact gccgtcagca
     8221 agcaaatgtg gcgggcaagg cggctgcacc ggaaatgctt tccgtcccga agtcagtccc
     8281 agtcgcctga ccaacatcac gttctacttg caaacgttga cggtgccggc gcgccgttcg
     8341 gctggtgatc cagaggtcaa gcgcggggaa tcgatattca ctgcactaca gtgcggcgtc
     8401 tgtcacgtac ctgaattgca aacaggcccc aaggctttta tcccggctgc ggcaaatgtg
     8461 accattcacc cttacacgga cctgctgctg catgacatgg gcgatgaact cgccgatggc
     8521 cggcctgatt tcgaagccag cggtcgggag tggcgtactg cgccgttgtg gggcgtcggt
     8581 ttgattcgta ccgtgaacgg caagctattc ctgctccacg atggccgcgc ccgggacgtc
     8641 gccgaggcaa ttctttggca tggcggacaa gcccaagcgg ccagtgaggc atttatctcg
     8701 ttgtccaagg ccgagcgcga cgcattggtg aagtttgtcg aatctttgta gtgccgactg
     8761 agcgtcgtta cctgcagccg tttggctggc gtcagatgat gccaccgtcg cgtaatcggg
     8821 agatgtcttc gcttgcgtat ccggcggcgc gcagaatttc ctcggtatgc tcacccgctt
     8881 ccggggcggg gcgttcgaca gcgaattcgt attcgctgat tttccacggc ggcgtgaact
     8941 gcaacgtacc gctttggcgc accaccatct cgcgcgcacg gatatgcgga tcctcaaaaa
     9001 tctcttccag cgtgagcacc ggactgacgc aacaatcttc accgctgaat tgtttgaccc
     9061 agtaagcctg cgattgcgcc gcaaatattg cactggtctg cgccttgccc cagtgcgcat
     9121 cgcgaccgcc tgacagtgca cgaatctccc agtgacaagc cttgaggtcg gggcgttgca
     9181 aaacatcgca cacacgatcc cagaatttcc gttccagcgc gcccacagcc atatggcggc
     9241 catcagcggt ccgatacacc gcatagcagg gttcacggcc cgacagaaaa tctccgccgc
     9301 gtgggggaga ctttccgttc gttgcaaccg cagccagaac ctgcagattg tggaccagga
     9361 cgccctcggt catggcgaca tcgacataac ggcccttgcc gctgcgctgc gcatcgagca
     9421 gcgcagccag gatgcccatc gctggcacca cagcaccgcc gagaatgtcg gcgatctgca
     9481 tattgggcaa gaccggattc ccttgctcac ttccgttctg atcgataaat ccggcgtagc
     9541 tgcaatagtt cacatcgtgg ccggcgcgtt cacggtaagg gccattttgc ccatagccgg
     9601 atatggcgca ataaacgatg cgcggattca cggcgcagca ggcggcatac ccgacgccaa
     9661 gacggtccac gacgccggga cggaaacctt ccaccaccgc atcggcatca cgtaccaact
     9721 tcagaaagac ttgcactcct tcgtgctgtt ttaggtcaag acgcagcgcc cgcttgttgc
     9781 gatttattgc agcgaacact ggcgccagtt tgccgggcag tgcgccgagc gtgcgcgcat
     9841 agtctccggc accggtatct tcgattttga tgacatccgc gccaaggtca gccagatgca
     9901 atgtgcagac cggtccggga agcaaccggg tcaggtccag aatgcgaatt ccggaaagcg
     9961 gttttgcgcg catcggtctt tatccggccg ttgctttttt ggggccggta taaaactcgg
    10021 gaaaacgctt cgcgatgacc gggctgtccg cggcccacgc gtggcaggaa tcgatgaggc
    10081 cagggcgctt gcgcacaact tgcgcccgct cgcgacgcgc tcgtaatgga taaatggttt
    10141 gaaatgcggt cgttgccagt ggaccgagca attccagcaa atgcgtgcag cctttggtac
    10201 cacccagcag ctcacgtgtc ttctgcgtcc atccaccctg gatcttcagc cccttcagca
    10261 cctggaagtt gggcgtgata tctccgcaca tgtgataagg cccccatacc gtacgagcct
    10321 ccacttcaat gacaacgagt tcatcattaa ccgtcagccg caaccacatt tcgtgcagcg
    10381 gctgcccgga cggggcggga ccgcgatcga ggtcattcaa atcgtagctt ttggtgtcga
    10441 ccagatggcc ttcgatgtcc caaaggccgt cttcccggtg gtagccgata cattcgatgt
    10501 ggcggcgatg catgagcttg cgtggagtcg cggtggtcaa tccggtattc aacgtgaagt
    10561 ccccgtacgg cggcgctctt cgatacgctg gagcactgca agtttttcgg atgcggaaaa
    10621 atccagccag ccggcaatct catccagggt gcgcaggcaa cctcgacaca agccattgaa
    10681 cggattcatc tgacagacgt ttacgcaggg agaagagatg ttgtttgcat cggcgttcac
    10741 gcgccggcct tgtcgcgttt cgcgctcaaa gcccggccgg ctcgagatgc cgtgggccgg
    10801 ccgagatggt ggcagatgaa ctgggccgca tcgagcaagc ggttcatgtc aattccggtt
    10861 ttgatatcca ggccattcag catatacagc acgtcttcag ttgcgacgtt gccggtggcg
    10921 cccctggcgt acgggcagcc gcccagtccg gccgcagaac tgtcgaaggt cgccacgccc
    10981 aactccatca cggcgaggat gttcgccaag gcttgcccgt acgtatcgtg aaaatgtccg
    11041 gccagcatgt cgatcggaat ccggcgcgca agcgtttcaa taagcttctg agcgcgcgca
    11101 ggcgtaccgg ttccgatagt gtcgccaagc gaaatttcgt aacagcccat atcccgcaat
    11161 tgtatggcca cctccgcaac tgcctgcggc ttcacttcgc cttcataagg gcaatcgagt
    11221 accacggaaa tgtaaccgcg cacccgcaag cccgctttgc gagcggcgtc gcacactgat
    11281 ttctgacgct tcatggattc cacaatagtg caattggtat tgcgcatgga gaaggcttcc
    11341 gtagccgaag cgaatcccgc cacttcggtg gcgcccgcgg caatcgcgga ctccagccct
    11401 tgcatattag gaaccagaac tggatacgca acgcccggcc tgcgccggat gcgcgccatc
    11461 acatctgacg tatcggccat ctggggcacc catttcgggc tgacgaagct gccagcttcc
    11521 acggactgca ggcccgcgtc cgacagccgg tcgatcaatt cgaccctgac ctgcgtcggg
    11581 accgaaacct tctcattttg caggccatcg cgcggactga cgtcgacgat ttttacctgg
    11641 ggcggatacc tcatgacttg cttacctgtt ccggttcgaa ctcgagcaat tgcacacctt
    11701 ccaccacctg ctgtccttcg ccgaagtata tctgcttgac cgttccgcgc cgtggcgccg
    11761 tcactgtatg ttccattttc atggcttcga tgatcatgag cgcttgtcct ttgactactg
    11821 tttctccgct ggagacaaga acgcggatga cgctgcccgg caaaggggac accagccgcc
    11881 ccgatggatc ttcttcctca atcacagctg ccttctcgaa cgcgagcagg taactgcggc
    11941 catcgcaaaa aacggttatg tccccggcgc gccgcacaac gcgcgcaggg ctgcgcctgc
    12001 catcgagttc gacattgaga gactggtcgt cggcgggcgt ggctgaagcc gacagccggc
    12061 gcccccccgg cagatcgagc agatacccgc cctcgcgata atgcgcgacg atctctttgc
    12121 gctcgccggc gtccacgaat acgaatgcat gatggtttcc ctggttcagg cgccacccgt
    12181 gccgtagatg ccatggcgaa taagggtccg gcgagtgtgc cgcggccgct gccgcttcac
    12241 tatccatgtg cagggtctca cttaaaaccg cgatcgccag aatagtgtcg gggaccgggg
    12301 cgtgttccgg aaaaagcgcc aagcggttgg actctatgag cgcggtatgc aacgcaccgg
    12361 ggtcgcgatc ggcatgtgca aacgccgaat gtgcagtgat cgcggacagc agcgatatgt
    12421 tagtgacgac gcctgtcacc tgatactgag ccagcgcggc gcgcagccgc cgcagcgcgg
    12481 acgggcgatc caggtcccaa gcaatcaatt ttgccagcag cggatcgtag tacacaccga
    12541 tttcatcgcc ttcctgaatg ccactatcca cgcgcacatg cgcactgggc ggcggcagcc
    12601 gcagatggtc aatccggcca gcgaccggaa tgaaatcgcg tgcggcgtct tcggcataaa
    12661 tgcgcgcttc gatggcgtga ccgtcgattg acagctgttc ctggcgcagc ggcaatgcct
    12721 cgcctgcagc cacgcgcagc tgccattcga ccaggtcgag gccggtgatc atttcagtta
    12781 caggatgttc gacttgcagc cgggtgttca tttccatgaa atagaaacga ccatcaggct
    12841 cggcaatgaa ttccaccgtc ccggcgccca cgtaattgac cgcctttgct gcagccaccg
    12901 ctgcggcgcc catttgcctg cggcgctcct cggtcatgcc gggcgcgggc gcttcttcca
    12961 ggatcttctg gtgacggcgc tgcactgaac agtcgcgttc aaacaaatgc agacaggtgg
    13021 cgtgcagatc ggcgaacacc tgcatttcaa tatgccgcgg acgctgcaga tatttttcta
    13081 ttagcaccgc atcgtcgccg aacgacgaag ctgcttcacg ttttgccgct gccaaggctt
    13141 gcgcaaattc actggggtgc ttcactgcgc gcatgccttt tccgcccccg ccggcggagg
    13201 ccttgatcaa caccggataa ccaatgcggg ccgcttcttc ttgcaatgtc gcgagaccct
    13261 gcgccgggcc gtggtagccg ggcaccaacg gtacgcccgc cttctccatc agcgccttgg
    13321 cggccgactt cgaacccatg gctgcgatcg cttcgggcgt tggcccgata aaaacgatcc
    13381 ctgcctgctg acaggcgcgt gcaaatgctg cgttttccga gagaaaaccg tagccgggat
    13441 gaatggcttt ggccccgctg cgggtggcca catccaggat gcgatctccg cgcaaataag
    13501 aatcgcgcgg gtgcgcgggt cctatcatcc aggcctcgtc gcaggcactg acgtgcatag
    13561 catgcgcgtc cgcgtgcgag tagaccgcga ccgtaccaac gcccaaacgc cgggcagtac
    13621 gcgcgatccg gcaggctatt tcgcctcggt tggcaatcag aatcttgtca aacatctcgt
    13681 tcgcgccgcc gcgaggcagc gcggcaagtg acaattattt ccatgtatcg tccacgtcgt
    13741 ccggggcaag cgcttccgct cggccgccgg gcaattgtga caggctgcga tgtagcagta
    13801 gcgcaagcgc gaaacacacg ctgaacagcg ccagtccgat gaagctcgcc gccattgcga
    13861 tatgtatgag cgtctcgatt gttccaccgc gcgcttcctc gcaggctgcc cagccttcgc
    13921 gcgttctgag cgcaatatcg gcaatcgccg ggccttgcgc ggatcgcagt tcgcccagca
    13981 gacgctcaaa gccgggcaat gtgaacttgt actgccccat gtaatgccac agccagaagc
    14041 ccgcgccaag atcgagcagg aacatcgcca agcccagtgc gcaaaataaa gccaggagtt
    14101 tggcccgttg cgcatcgacc gccgcatctg gatgcatgtg gactggcatc ttttacttcc
    14161 aatccggaat gcgtttctcc acgaaagcgg cgatcccttc ctgtccttca ctatcgacgc
    14221 gaattgcagc gatgcgccgg gcggtttcca tggcgagctt atcgtccatc ggccgcctgc
    14281 ccatgtccgc gatcagttcc ttcgcggacg cgagggcccg cggaccgcct ttcagcaagt
    14341 gccccgccat gctggcgacc atttggtcca gctctgcacc cgcaacgact tcgtgtacca
    14401 gtccgatgcg cctggcttcc agtgcgtcga agcgttcggc cgtcatgaaa tagcggcgtg
    14461 cggcgcgctc gccaatcgcc gcgatgacat acgggctgat aaccgaagga atcaagccca
    14521 aacgcacttc cgacagcgaa aagctggact gcgtgctggc gattgcgata tcgcaacaag
    14581 ccaccagacc gactccccca ccgaatgcgg gtccctgcac tttcgcgatg gtcggtttgt
    14641 tcatgccatc cagtgtgcgc atcaaagcgg caagcgccat cgcatccccg agattctctt
    14701 cgtgggaata cttggccatg cgtcgcatcc aattgagatc ggcccccgcg gagaagctct
    14761 tgccgtttgc agcgagcacc accacgcgta cggcaggcag ccggtccagc ctgcgcagtt
    14821 cagcggttag cgcagcgatg agtacatcat cgaacgcgtt atgcacatcg ggacgattca
    14881 tgcgcaacgt tgccacacct gcggccaact cggtttgcaa gatctcctga gtcatggtca
    14941 ttcccgtctc agtcgggccc gaagcgtttg gcgacttctt ccagcatgac ttccgtcgcg
    15001 ccaccgccga ttgccagaac gcgtgcgtcg cgcgcctgcc gctcaatcgc ggattcccgc
    15061 atgtacccca tgccaccatg gaattgttga caggtgtaga ccacttcatt gacgagctcg
    15121 ccggtcagtg ccttcagcat ggagacctct cgcacgcact cacgtttttg cgccgcgagc
    15181 cagccgcaat ggtaaagaaa ctggcgcgct gcatgggtct ttgcctccag catggccagt
    15241 cgctggcgaa ttacctgctt gtcgaacagc acgcccccga aagccttgcg gttcttgacg
    15301 tattcgatgg ttaaacgcag cgatgtcagg gcattgccga ctgccatcgc gcccgcggcg
    15361 atacgctcgc gttgaaagtt cttcattacg gcatagaaac ccgcattttc ttcgcccagc
    15421 agattttcag caggcacgcg gcaatcttcg aaaaccagct ctgcggtgtc cgagcacaac
    15481 cacccggtct tgtccagcgc acggccgacg cggaaaccag gcaggccttt ttcaacaatg
    15541 aacatcgaaa tgccgcgtga acccttggct tgcacgtcgg tacgcgcggc gacgaaatac
    15601 aaattggcat gcacgccatt ggtgatgaac atcttggcac catttaatac ccattcattc
    15661 ccgtctcgca cagctcgagt gcggataccg gccacgtccg acccggcgtc cggctcggtc
    15721 accgcgacag ctgtgattgc ttcgccacgg atgattccgg gcagatacct gtcgagttgc
    15781 gctttcgatc ccgcgttcac cagatgcggt gaggccatgt ccgaatgcac cagcgccgtc
    15841 acgacgaatc ctccgaaggt gctgcgggaa agtgcctcag ccaccacgag cgcgctcatc
    15901 gcgtcggctt ctgacccacc gtattcaacg ggataggtaa tgccgaaaaa gcccaattca
    15961 cccatcttgc gcaagacctc acgcggcacg cagccctgtg tctcccacgc cttgccggaa
    16021 ggttcaactt ccttgtcgat gaagcgcgcg acctggtcgc gcagggcgac atgttcttcg
    16081 ttcagataaa tggattccat gttgggtgag tatgagcgcg attacatccg gaacacgccg
    16141 aatcgcgtcc gttcgatggg cgcattcagc gcggccgaca gaccgagacc caataccatg
    16201 cgcgtgtccg caggatcaat cacgccatcg tcccacaacc gtgcgctggc atagtacgga
    16261 tgaccctgga cctcatattg tgcgcggata ggcgacttga acgcctcttc atcttcccgg
    16321 ctccacgtct tgccggattt ttcaatgcca tcccgtttga cctgcgccag cacgctcgcc
    16381 gcctgttcac cgcccatcac ggagatgcgc gcattcggcc acatccacag gaagcggggc
    16441 gaataggcgc gaccgcacat gccgtagttg cccgcaccga aactgccgcc gacgatgacg
    16501 gtgaacttgg gcacgcgggc acaggagaca gcggcgacca atttggcgcc atccttggcg
    16561 atgccccctg cctcgtattt ctttcccacc ataaaaccgg tgatattctg caggaacaca
    16621 agcggtacgc cgcgctgtgc gcacagttca atgaaatgag cccctttcag cgcggattca
    16681 gagtaaagga tcccgttgtt cgcaataatg ccaaccggat acccgtggat gtgcgcgaaa
    16741 ccacacacca acgtgctgcc atacagcgcc ttgaattcat cgagctcact ggcatcgacg
    16801 acgcgtgcaa cgacgtcccg gatatcgaac gatttacgcg tgtccgcggg aacgataccg
    16861 tgcaactcgg ccgcttcgta cagcggtgcg cgcggttcac gcagctcaag cgctatttct
    16921 ttcttgcggt tgagattgga aacgatgcga cgtgtcagtg caagcgcatg ggcatcgtcg
    16981 ttcgcatagt ggtctgccac gccggatact ctggcatgta catcggcacc gcccagttct
    17041 tcggcgctga cgatttcacc agtcgcagcc ttcaccagcg gcggtccggc aaggaatatc
    17101 gtgccctggt ttctcacaat gatcgattcg tctgccatcg cgggcacata agcgccgccg
    17161 gccgtgcacg aacccattac acacgcaatc tgcgcgatgc ccatcgcaga catgttggcc
    17221 tggttgtaga agatgcgacc gaagtggtcg cgatccggaa aaacctcatc ccaggtcggc
    17281 agattggcgc ctcccgaatc caccaggtaa atgcaaggca ggtgattttg ctgcgcgatt
    17341 tcctgcgcgc gcagatgttt tttcacggtg atcggaaaat aggtcccgcc cttgaccgtc
    17401 gcatcattgg ccacgaccac gcactcgcgt cccgcgatcc tgccgactcc ggtcaagatg
    17461 cccgccgaag caacctgatt gccgtacatg ttccacgcag ccagttgcga cagttccaga
    17521 aacggcgaac ccacgtccag gagccgccga acacgttcgc gcggcaatag cttgccgcgg
    17581 ccgatgtgct tttcccgagc agcctcacca ccaccctgat cgataagctc gattttcgcg
    17641 cgcaagtccg ccaccatcgc gcccatggct tcctgccccg ctttgaactc cggcgagcgt
    17701 ggattaactt tgctctgtat aacactcatt gaggcattac gagcgcttcg gaatgggatg
    17761 gcccgggaga agttcgtatg ggtgtttcca gcgcggctcc gaccggatgc gctgaagcca
    17821 gtcgttaatg ctcggatact gcgcccaatc cacgcccagc tcatcggcga aaaacagata
    17881 gccgcatgcg gagatgtctg cgatggtcat gcgcccgccc accacatagc ggttgcgtgc
    17941 cagatgtgca tccaggattt tccagctggc ttcagcacgt ttgcgaaatt cttctacgac
    18001 aaccggattc gggttgggca tcagcacccg cagaaagcga tatgtcgccg tatagctggt
    18061 gagtttgtgg ttgtcccaca gcagccagcg caggatttcc cgccgctctt cattgttgtt
    18121 ccagccatac ttgccaaatt gctcgaccag ataatctagg atcacaccgg actggctcag
    18181 atcatgtttg ttgtgctgga gcaccggaac ctcacccatc acgttgattg cgcggtacgc
    18241 gggagtgcgt gtttcgccat tgaaaaaatc gacaaagcgc ggcgcccagt ctgcaccggc
    18301 cagttccaga taaagtgccg gcttgtagga attgcctgat tgcgcgaatc cgtaaagcag
    18361 atattcggcc attgccttac caacccccgt tctgcaacca gcgatcgacg tgctccaggc
    18421 gatcgaagcc ccagaatggt tcgccatcca caatgatata aggcgatccg aacacgccgc
    18481 tggcgatagc agcttcggtt tcgaccttga gcttttcttt catgacggga tccgcgatcg
    18541 cgtcgagagt tttctgtcga ttcaggccca cggatgcggc aacatcggcg gtaatctccg
    18601 gactggagat atcgcggtca tccaccatgt aggcgcggta cagcgcaagc gttacttctc
    18661 cctgccgttc cgcaccttct gcttccaatg agtagatcac gcgtgacggg gactggccgg
    18721 cgattggaaa tttggttgga agcttgtacg ggatgttgaa caggcgcgcg cagcgttcca
    18781 tatctcgccg gctgtagtcg cctttcaggg gtgcattcgg caacggcaca ccaccagtca
    18841 ccttgaatac cacgccgagc agaatcggct tccagtcaac ggtgcggcca tgcttcgcgg
    18901 ctagcgccgt aatcctggtc ccggccatgt agccgtaagg cgaagcgaaa tcgaagtaga
    18961 actggattgg attggccatt tagtctgtcg tcttttgtga atggctaggc gtgcggggga
    19021 aatgtcgtgt tgttaagttt cgctactgct ggtaccagcg atgccacaag atggtccggc
    19081 ccaccgaaac cgagattcac cgcctgggtc agcgtctgca gagtcgctgc cgccatcgac
    19141 ccagcaacac cgaattccat tgccatctgg ctgtaataac gcacgtcctt gagcgcgttg
    19201 gcgaggccga atttcatgcc gccaaaatcg ccttccactg cctttcccgc catcatctga
    19261 aaaatcccgg aattggccgc acccgatgac accagcgcga agtatttttt cggatcaacg
    19321 ccgagcttgg cgcatgcggt caacgcttca gcgatcaacg ccatctggcc gatggccgcg
    19381 caattattga tgagtttgat cttgtggccc gctccaacat cgccaacatg gaaaatattc
    19441 tcgcagaagg acttcagtgc tggttcaatt tcgaggaacg tcgcttgatc ggcgcccacc
    19501 atcgtattca ggcggccttg ttcggcttcc acgggggtac gagtcagcgg cgcatccgca
    19561 aaacgaacgc ctttcgagct gaggtccgcg gcgatcttca gcgtggaacc gggctggctg
    19621 gttgaagtat ccacaacgat ctggccgttc actaccgatt ccagaatgcc ggccttgcca
    19681 tagacgaggt cttcgacctg cgctgatcct gtcacgcaca aaaaaataag ctcgctgctt
    19741 tttaccaggt gcgcaacatc ttttgcttcc ttggctcccc gcttgaccag atcatcgacc
    19801 ggcacgcgat tccggtggcc aagcaccgtc gtcggaaagc ctttttctga gagatttttc
    19861 gcgatgccat gacccattaa accgactccg atgaacccga ttttcttgtt cgccattgcg
    19921 ctttcctctg aagaaaaaag acctcaatac cacgtcgctg gtgcgccctc acgaggcgca
    19981 gcggccggcc agtgtgttat ttgataagct cgattgccat cgccgtggct tccccgccac
    20041 cgatgcaaag tgacgcgacg ccgcgtctga gaccacgctt tctcagcgcg ccaatcaatg
    20101 tgactacaat gcgcgcgccc gatgccccga tcgggtgtcc cagcgcgcaa gcgccgccat
    20161 gcacattgac tttctcatgt ggcaacttat gttccctcat cgcggccatg gttaccacgg
    20221 caaaggcttc attgatctcg tagagatcca cctggtcaga agtccagccc gtactgtcga
    20281 acagtttgcg catcgcgccc accggggcgg tggtgaacag tgccgattcc tgcgcgtgcg
    20341 ttgcgtgccc aataaccgtc gc
//
LOCUS       DATMZQ010000206         6065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4568567, whole genome shotgun sequence.
ACCESSION   DATMZQ010000206 DATMZQ010000000
VERSION     DATMZQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4568567"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="glmU"
                     /locus_tag="VN496_06685"
     CDS             complement(<1..1191)
                     /gene="glmU"
                     /locus_tag="VN496_06685"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXF16671.1"
                     /translation="MLAPPVNKDLNVVILAAGKGTRMRSRLPKVLHAIGGKPMLGCVL
                     DRAATLRPARTIVVYGHGGDAVPTAFPGAGVEFVLQEPQLGTGHALQQTVPYLYGDGS
                     TLVLYGDVPLTRDATLAKLIAKPGLRLVTAVLPDPTGYGRIIRDKEGHIVHIVEEKDA
                     NAREKTIQEINTGILCAPTDMLRQWLPKLSNRNAQKEYYLTDIVSLAVDCGTEVSAVQ
                     PSDIWEVLGVNSTEQLAYLERVHQLQNAKDQMARGVTLADPWRFDVRGELECGIDVFI
                     DVNCVFEGKVRLADDVRIGAGCVLRDVEIGPGSELAPYSHLDGTVIGSHCRVGPFARM
                     RRGTRLGDDVRVGNFVEIKATELGNKSKANHLSYLGDTMVGSRVNIGAGTITCNYDGA
                     NKYRT"
     gene            1667..2311
                     /locus_tag="VN496_06690"
     CDS             1667..2311
                     /locus_tag="VN496_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXF16672.1"
                     /translation="MLNLLETINEQISAQSLPLVAITVAAVPCPDTPLILTLHWHGFI
                     EEKICEIEEATTVAYTAIPSSALQINRRWSDLAIVDREAMEAGWELGAWDVARAERPG
                     CTRPGAQSREALECLQAFGTFPFGPGGDPQAVVAEAPDIDELINLAAHKGYLMWMFRP
                     VHRGIWAEFADDATLGEDGTRTPPCPVLPVPPAGQGPARTVYRFGVPNPVTLAH"
     gene            2498..2791
                     /gene="groES"
                     /locus_tag="VN496_06695"
     CDS             2498..2791
                     /gene="groES"
                     /locus_tag="VN496_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXF16673.1"
                     /translation="MKIRPLHDRVIVKRLEEERKTASGIVIPDTAAEKPDQGEVMAVG
                     NGKVLEDGKVRPLDVKKGDKILFGKYSGQTVKVHGEELLVMREEDIMGVVEAA"
     gene            2832..4481
                     /gene="groL"
                     /locus_tag="VN496_06700"
     CDS             2832..4481
                     /gene="groL"
                     /locus_tag="VN496_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185913.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXF16674.1"
                     /translation="MAAKDVKFHDSARSRIVAGVNILADAVKVTLGPKGRNVVLERSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVQE
                     GMKFVAAGMNPMDLKRGIDKAVNTIVEELKKISKPCTTSKEIAQVGSISANSDSVIGQ
                     KIAEAMEKVGKEGVITVEDGSGLEDELEVVEGMQFDRGYLSPYFINNPDRQIAILENP
                     YVLLHDKKISNIRELLPILEQVAKAGRPLLIVAEDVDGEALATLVVNNIRGILKTCAV
                     KAPGFGDRRKAMLEDIAILSGGTVIAEEVGLSLEKATLKDLGSAKRIEIGKEETTIID
                     GAGDGKTIEGRVKSIRKQIDEATSDYDREKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVAFIRSRSSIAKLKGDNADQDAGIKIVVRALEEP
                     LRMIAANAGDEPSVVLNKVAEGKGNFGYNAATGDYGDMVEMGVLDPTKVTRYALQNAA
                     SVAGLILTTAAMVAEAPKEEHSHGGGGGGMGGMGGMGGMDM"
     gene            4700..>6065
                     /locus_tag="VN496_06705"
     CDS             4700..>6065
                     /locus_tag="VN496_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF16675.1"
                     /translation="MTGVATHVVDKNHPDFKPEEKRVIFASSLGTVFEWYDFYLYATL
                     APFFAALFFPKGNETAALLSAFATYAAGFLVRPFGALVFGRIGDLVGRKYTFLITIIV
                     MGASTFAVGLLPTYQSIGWLAPILLVSLRLLQGLALGGEYGGAATYVAEHAPNNRRGY
                     DTSWIQTTATLGFFLALLVIGVCRSQIDAKEFADWGWRIPFWVSVFLLGFSVYIRLKL
                     EESPVFKRMKEEGRGSKAPLTDSFLRYPNNKFVLLALLGATAGQGVVWYTGQFYALFF
                     MIITLKLDYLTTYMLIGASLIIGTPFFIFFGWLSDKIGRLKIILAGCLIAALTYFPLF
                     HGLTHYVNPALEQYQQNTKISVAATDCSFHIFVGPWSKFTDCDRAKDYLTKQGLSFSS
                     LPGIPGKTVVTKIGEVEIEGWDDKKMSETLKASGYPGAADKTKVNYPMTLLLLVIMLI
                     YVTMV"
BASE COUNT         1412 a         1573 c         1728 g         1352 t
ORIGIN      
        1 ggttcgatat ttgttggcgc cgtcgtaatt acacgtaatg gtaccagcgc cgatattgac
       61 gcgactgccg accatcgtgt cgccgagata gctcagatgg ttcgctttgg atttgttgcc
      121 gagctccgtt gctttgatct caacgaagtt gccaacccgg acatcatccc caagtcttgt
      181 cccgcggcgc atacgcgcaa acgggccgac gcggcaatgg cttccaatta ctgttccgtc
      241 cagatgggag taaggtgcga gctcgcttcc tggtcctatt tccacgtcgc gcaacacgca
      301 acccgcgcct atcctaacgt catctgcaag ccgtactttg ccttcaaata cgcaattgac
      361 atcgatgaag acatcgatgc cgcattccag ttcaccgcgc acatcgaaac gccaagggtc
      421 ggccagcgtt acgccccggg ccatctgatc cttggcattc tggagttgat gaacacgttc
      481 caggtaagca agctgctcgg tgctatttac gcccaagact tcccagatat ccgatggttg
      541 taccgcgctc acttccgtgc cgcaatcgac ggcaagcgat acgatatcgg tcaggtagta
      601 ttccttttgc gcattgcgat tcgataattt gggcaaccat tgccgtagca tgtcagtagg
      661 cgcgcaaaga ataccggtgt tgatttcctg gatcgtcttt tcccgagcgt tggcgtcctt
      721 ttcttcaacg atatggacga tatggccttc tttatcccgg ataatccggc cgtaccccgt
      781 tggatccggc aacactgctg ttaccagtcg caaacccggc ttcgcaataa gcttggctaa
      841 agtagcatcg cgagtaagcg gcacgtcgcc atatagaacc agtgttgaac cgtcaccgta
      901 caaataaggc actgtctgct gaagggcatg cccagtgccg agttgcggct cctgaaggac
      961 gaattcaacg cccgcgccag ggaatgcggt cgggactgcg tcaccaccat gcccatagac
     1021 gactatcgtg cgtgccggac gtagcgttgc cgcccgatca agcacgcaac ccaacatcgg
     1081 cttgccacca attgcatgta ataccttggg taacctggaa cgcatgcgcg ttcccttgcc
     1141 ggcggcaagg ataacgacgt tgaggtcttt gttcactgga ggcgcgagca tggcagaaaa
     1201 ttatcgcaca agctcgcgaa ttgtccgctg ctttgactct gcacgttatg tcgattggga
     1261 cgaaaaaatc ttgtgcgtcc gttctgacga tgcattgttt catgttccgt ttgctaatgg
     1321 cgccccgaaa gttggtccgt aaggaccaga agataaatcc ccagatggtg ccattgcgtc
     1381 gaggattcgg cggaattccg agcggctaag tattaagcct ggagaggcgt gccatccgat
     1441 tgcagttgtc tacgtgcgga aatagaaaat gccaccctgc ggcttcgcgc gatccactaa
     1501 ctaagctgtt gcaagataca tagaaattcc catggaggct gcccggaaaa cgcaagtcaa
     1561 ccgcaccaac gtgtcaaaac ggatcatccg gctcatgcta tagcatcggc aggcatcctt
     1621 gatgctttag agtgtcgtta acgtgcctct tcaaaggaga tagcggatgc tgaacttgct
     1681 ggaaacgata aacgaacaaa tctcagctca gtcgttgcct ttggtcgcca ttaccgtggc
     1741 ggcggtcccc tgtccggaca ctccactgat cctcacacta cattggcacg gcttcattga
     1801 agagaaaata tgtgaaatcg aagaggccac gaccgtggcg tacacggcta tcccgagttc
     1861 tgcgctccag atcaaccggc gctggagcga tctggcgatt gtggatcgcg aagccatgga
     1921 ggccggctgg gagttggggg cgtgggatgt ggcaagggca gaaaggccgg ggtgcactcg
     1981 cccgggggcg cagtcgcgtg aggcgttgga atgcttacag gctttcggca catttccctt
     2041 cgggcccggt ggggatcctc aagccgtggt ggcagaagcg cccgatattg atgaactcat
     2101 taatctggca gcacataaag ggtacttgat gtggatgttc cgcccggttc accgcggaat
     2161 atgggcggaa ttcgctgacg atgccacctt gggggaggac ggcacgcgga cgccgccatg
     2221 tccggttttg cccgtgccac ctgctggcca aggcccggct aggaccgttt accggtttgg
     2281 cgttccgaac ccggtaactc tcgcgcacta atttttctgc tcaacactgg tgcggcgctt
     2341 ccttgatgta agcgcacgct cactacctta ctcgaggcta attctacaaa gcccttgagt
     2401 tctatggtcc ccgaccctat tattagcact ctcaaagatt gagtgccaaa gagggctata
     2461 tcgccctcta acgtcaatta aaaaaggaga cttgaaaatg aaaatccgtc ctttgcacga
     2521 tcgcgtcatc gtcaagcggc tggaagaaga gcgtaagacc gcttcaggca ttgtcattcc
     2581 ggataccgca gcggagaagc cagaccaggg cgaggtaatg gcagtgggca atggcaaagt
     2641 cctggaagat ggcaaggtcc gcccgttgga cgtcaagaag ggcgacaaga ttctattcgg
     2701 aaaatattcc ggccaaacgg tcaaggttca cggcgaagag cttcttgtga tgcgtgaaga
     2761 agacatcatg ggcgtcgtcg aagcggccta acctccaagc aagttcctac cctattcagg
     2821 agtaaattct tatggcagca aaagacgtca aatttcatga ttccgcacgt tcccgtattg
     2881 tggctggagt aaacattctg gccgatgcag tcaaagtcac gctgggccca aagggccgca
     2941 atgtcgtcct ggagcgttca tttggcgccc cgaccattac caaggacggt gtgtcggtcg
     3001 ccaaggaaat cgaactaaag gacaagttcg agaacatggg tgcacagatg gttaaggagg
     3061 tcgcatccaa gacctccgat gtagcgggtg atggcactac tactgcgacc gtcttggcgc
     3121 aggccatcgt gcaggaggga atgaagttcg tggccgcggg catgaaccct atggacttga
     3181 agcgcggcat cgacaaggcg gtcaatacca tcgtcgaaga gctaaagaaa atctcgaaac
     3241 cgtgtacgac aagcaaagaa atcgcgcagg tcggctcaat ctccgcgaac tcggattcgg
     3301 taattggcca gaagatcgcc gaagccatgg aaaaagtcgg caaggaaggc gttatcacgg
     3361 ttgaagacgg ctcgggcttg gaagacgagc tggaagtagt cgagggcatg caattcgacc
     3421 gcggctacct ttctccatac ttcatcaata atcctgatcg gcagattgca atcctcgaaa
     3481 atccttatgt cttgctgcac gacaagaaga tttcgaatat ccgcgaactg ctgcccatcc
     3541 tcgaacaggt tgcaaaagca ggccgcccgc ttctgatcgt cgccgaagac gtcgacggtg
     3601 aggcattggc gaccctggtg gtgaacaata ttcgcggcat tctgaaaacg tgtgcggtga
     3661 aggcgccagg tttcggtgat cgccgcaagg ccatgctgga agacatcgcc atcctgtcag
     3721 gcggaacagt catagcggaa gaagtgggtc tttccctgga gaaggcaacc ctgaaagatc
     3781 tcggatcagc caagcgcatc gaaattggca aggaagaaac caccatcatc gatggcgcgg
     3841 gcgatggcaa gacgatcgaa ggaagggtca aatccatccg caaacagatc gacgaagcca
     3901 ccagcgatta tgatcgtgaa aagctgcagg aacgcgtggc aaaactggcc ggcggcgtag
     3961 cattaatcaa agtgggcgcg gcgacggaag tggaaatgaa ggaaaagaag gctcgcgtgg
     4021 aagatgcgct acatgctacg cgtgcggcag tcgaggaagg tatcgtggcc gggggtggcg
     4081 ttgcattcat tcggtcgaga tcttcaatcg ccaagctgaa gggtgacaat gccgaccagg
     4141 atgctggaat caaaatcgtg gtgcgggcac tggaagaacc cttgcgcatg atcgctgcca
     4201 atgcaggcga tgagccgtct gtggtgctga acaaagtggc cgaaggcaag ggtaatttcg
     4261 gctacaacgc tgctaccggc gattacggcg acatggttga aatgggcgtc ctggatccca
     4321 ctaaagtgac gcggtacgcg ctgcagaatg cggcttcggt cgctggactg atcctgacca
     4381 ctgcggcgat ggttgccgaa gcgcctaagg aagaacattc ccatggtggc ggtggcggtg
     4441 gcatgggcgg catgggcggc atgggcggca tggacatgta agggtttagc tcaggcggtt
     4501 tggaaaagcc ccgctttcgc ggggcttttt ctttttgcgc aggggaaatc aacagtcgcc
     4561 gacacacggc tggcgatttg ctgcaattgc gctgaagcat gacgttgaca attgcccggg
     4621 gcgtgcaatt acactgtcgg tcgggcgacc ctaggccacc cggctgtcgc ggcgaaaaat
     4681 taccagggag gagaaaaaca tgactggagt cgctacccac gttgtcgaca aaaatcatcc
     4741 ggacttcaag ccagaggaga agcgggtcat atttgcctca tccctcggca cagtattcga
     4801 gtggtacgac ttttatcttt acgccacatt agcgccgttt tttgcggcgc tgttttttcc
     4861 gaagggcaat gagactgcag cgctactttc tgcttttgcc acctatgcgg cgggcttcct
     4921 ggtgcgaccg tttggtgcgc tggttttcgg acgaatcggc gatcttgtag gccggaaata
     4981 cacctttctg attaccatca tagtcatggg tgcatcgaca ttcgcggtcg gtcttctgcc
     5041 tacctatcag tcgataggct ggcttgcacc aattcttctc gtgagcttgc gattgctgca
     5101 ggggttggcg ctaggtggtg agtacggtgg tgcagcgacc tatgtggcag agcatgcgcc
     5161 gaataatagg cgcggatatg acaccagctg gatacaaacc acggcgacgc ttggcttttt
     5221 cctggcacta ctggtcatcg gcgtttgccg ttcacagatt gacgccaagg aatttgccga
     5281 ctggggctgg aggattccgt tctgggtctc cgttttcctg ttgggattct cggtgtacat
     5341 aaggctgaaa ttggaggaat caccggtatt caaacgcatg aaggaagaag gcagagggtc
     5401 aaaggcccct ctgacagaca gtttcctgcg ttacccgaat aacaagttcg tcttgctagc
     5461 cctgcttggt gcaacggcgg gacagggcgt cgtctggtac acaggccagt tttacgcgct
     5521 gtttttcatg atcatcacgt taaaactgga ctatctcacg acgtacatgt tgattggcgc
     5581 gtccctgatc attggtactc cgttcttcat tttctttggc tggctctccg acaagatcgg
     5641 caggctaaag atcattctcg ccgggtgcct gatcgctgcg ctgacgtatt ttccgctgtt
     5701 ccacgggctg acccactacg tcaatccggc acttgagcaa taccagcaaa acaccaagat
     5761 ttctgtggca gccactgatt gcagtttcca tatattcgtc ggtccgtgga gcaaattcac
     5821 agattgcgat cgtgcaaaag actatttgac aaaacagggc ctgagcttca gttcgttgcc
     5881 gggaattcca ggcaagacgg ttgttaccaa aatcggagag gtggagatcg aaggctggga
     5941 tgataaaaag atgtctgaaa cgctaaaagc ctctggctac cccggcgcgg cggacaagac
     6001 caaggtgaat tacccgatga ctttgctgct gctggtcatc atgctcattt atgtcaccat
     6061 ggttt
//
LOCUS       DATMZQ010000207        14119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1195228, whole genome shotgun sequence.
ACCESSION   DATMZQ010000207 DATMZQ010000000
VERSION     DATMZQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195228"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VN496_06710"
     CDS             <1..767
                     /locus_tag="VN496_06710"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXF16676.1"
                     /translation="DRIYGAANYLVYSRLKLTLAADWNDTDHGEASRDSLDSKINNTT
                     ATVNWITPAENTIGAQFRNAKARYPNSAIVGTSLVQNDYTENEYSLVTYWRLTGASEI
                     RARLGHTDRSFEQAPNRDYSSPTWRLNYIWRPTGKTAVDFSTWRELSDFQNLSANYVR
                     ITGISVIPTWSLAPKLALRGKISYEKLDYLGDPGLTAPAAQREDKDKIYQVSLLWTPW
                     RLTELDFNLETGRRTSNQALADFQYNAISFLATRYF"
     gene            complement(806..1828)
                     /gene="galE"
                     /locus_tag="VN496_06715"
     CDS             complement(806..1828)
                     /gene="galE"
                     /locus_tag="VN496_06715"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HXF16677.1"
                     /translation="MTASQSILVTGGAGYIGSHTVVELQAAGHEVFIADNLCNSTSEV
                     IDRIETISGRRPGFANLDMRDLGGLRKLFSDHSFHAVLHFAGLKAVGESVSNPLAYYD
                     NNVTGTLFLLEAMDEAGVKTLVFSSSATVYGNPHAVPITEDFPLSATNPYGRSKLIIE
                     DILRDTARADSQWRVALLRYFNPVGAHASGLIGEQPRGTPNNLMPYVAQVAAGILPRL
                     SVYGADYPTPDGTGVRDYIHVVDLAQGHLAALNVLAEQADLLTLNLGTGRGYSVLEMI
                     KAFSEASGRTINYRIVDRRPGDVAACYADPTLAFKVMGWRANRGIDSMCADAWRWQKA
                     NFLPSD"
     gene            2182..3162
                     /locus_tag="VN496_06720"
     CDS             2182..3162
                     /locus_tag="VN496_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HXF16678.1"
                     /translation="MKLLIKQVDNDGRLALIPEYVKTSWTIDVVNHEDRNELKGKLLD
                     ADAAISMNWPRDMPPAPKLRLLQLPGAGTDDIIFDAVPAQVAVCNVFEHEIGIAEYVL
                     SAMLQLTIGIPRMDAAIRRGEWYGSHLTGPRHGELYNQTLAIVGYGRIGHEVAKRARA
                     FGMNVLACSRTPRAGDGFVDEVGSMQQLDTLLGKADFIVLAVPLDASTLNLIDESRIA
                     KMKPSAFIVNVARGALVNEKALYEACKHRRIGGAAIDTWYRYPTDGKVSGDPSNMPFR
                     ELENVIMTPHGSGWTHALLPRRCRLIAQNLDRLARGEPLLNVVRAVAVAH"
     gene            3414..3971
                     /locus_tag="VN496_06725"
     CDS             3414..3971
                     /locus_tag="VN496_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXF16679.1"
                     /translation="MLEKRVFATGATISLDNNVMNVAEAEFAFRIGKDLPPKKGTYSR
                     EQVFAAVESMHPAIEIPDSRYRDYVTAGAAQLIADNACASYFVLGPAVTFKDWRGHDL
                     VRHPVTVYVNGNLARQGTGENVLGDPRIALTWLANELSLVGDTLREGQTVTTGTCITP
                     AAVVGGDTVNADFFEFGRVEAKLAR"
     gene            4128..5573
                     /locus_tag="VN496_06730"
     CDS             4128..5573
                     /locus_tag="VN496_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF16680.1"
                     /translation="MQLYYPRSFLKLILAGFSLAVLPLIFALINNAYSIHQLATHSQR
                     AVYQAVQTTQSTRFLIEQITSMERSIRQFAIIGDPSLLQGYGLAHERFADTARKMTAL
                     PLDDKQKIAMQALRNRETALFERIGKSGAKSETVNVAATELAALSEAARRIDEQGNSL
                     VDREVDTMQKMAGEVQRFIYWQLLALVPIALLLVIGATMLISKPIAQLESAIRSLGEG
                     SFGERIAVSGPADLESLGRQLDWLRLRLLDLEEQKSRFLRHVSHELKTPLTALREGVE
                     LLSDEVTGKMTAGQFEIARILRHNTLRLQKLIEDLLSYHSAQFHTSALSFSQFNLKGL
                     TARVAQQHSLAMRSKNLSLNLAASELNVEADENKLEAIVDNLLSNAIKFSPAGGTVSV
                     QLRRRGLDVVMDVVDQGPGILPQDRDRVFEAFYQGGSEYTGPVKGTGLGLAIVKEYVS
                     AHHGSVEIIVDGNPGAHLRVILPSRQVEAAA"
     gene            5735..6160
                     /locus_tag="VN496_06735"
     CDS             5735..6160
                     /locus_tag="VN496_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16681.1"
                     /translation="MKGGELTHEFETVREAFENDKSQLNRMKLALLLSYPGSSFRDDN
                     AAITLLGPFIKEKAQETSSLRPLAVWLNSELLEVRRAEEASQQYVSKLKDEQRRAEAL
                     QQKIETSQQKAEALQQKLDAILEMEMKMIEREQSIPKKK"
     gene            6157..7509
                     /locus_tag="VN496_06740"
     CDS             6157..7509
                     /locus_tag="VN496_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXF16682.1"
                     /translation="MIMSKARILIVDDDPDLLRLLTFRLEGADYAVESADSAERALAR
                     FSVAMPQLVITDLRMSGMDGMALFENIHRSNPTLPVIILTAHGTIPDAVAATQRGVFG
                     YLTKPFDSKDLLQQVEKAVAVSGGRNEKMNGEADWRVEIITRSLAIESILGKAKLVAA
                     SDASVMVRGESGTGKELLARAIHRASSRAERPFVAINCGAIPEQLLESELFGHMKGSF
                     TGAARDYKGLFQSAEGGTVFLDEIGDMPLPLQVKLLRVLQDREIRPIGTTQTIPVDVR
                     IISATHRNLEEAIKGGTFREDLYYRLNVVSFALPALCERREDIPVLATHFLHQLASRY
                     KKTLNGFASEAMEILVKSAWPGNVRELYNVVEQAVALSTTPIITPALVDSAMRGQIAE
                     LSSFESARSEFERDYLAKLLKITGGNVTQAAKLAKRNRTEFYKLLQRHHLDPKLFKAA
                     "
     gene            7727..8302
                     /locus_tag="VN496_06745"
     CDS             7727..8302
                     /locus_tag="VN496_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16683.1"
                     /translation="MTKTESPALTPLLVAATISVLAFSGIGVAAITGHLSITRSSLNP
                     FSSFSNSAASGLIQAPVVATAAHQGLTRHRGETVVDGKPVNYKFGARVPSRKIVCPDC
                     GVVDSIKPGQARPVGTGFTRSQGLESEGAAILKVGGQSGYDPVASGGAGFVVTVHMEN
                     GTVRTIYEPERPSFSIGERIKLVNGSVIRIG"
     gene            8535..8777
                     /locus_tag="VN496_06750"
     CDS             8535..8777
                     /locus_tag="VN496_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16684.1"
                     /translation="MRKLLLLLAAGLLAACAPAKKEANVNLAGYSPAFRAGYGDGCES
                     SKRTAGQTRDEDRFKHDAQYALGWRDGFDICGRKKK"
     gene            8916..10106
                     /locus_tag="VN496_06755"
     CDS             8916..10106
                     /locus_tag="VN496_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXF16685.1"
                     /translation="MNAPLARPAFHWDDPLLLEEQLNDDERMVRETARAYCQDKLMPR
                     VVSAHRQERFDREIFSEMGNLGFLGSTLEGYGCAGVNHVCYGLIAREIERVDSGYRSM
                     MSVQSSLTMYPIYRYGSESQRQKYLPKLASGESIGAFGLTEPDFGSDAGSMISRARKV
                     DGGFVLKGAKMWITNSPVADVLVVWAKDDRGMIRGFILERGMKGLATPKIEGKFSLRT
                     SITGEIVMDDVFVPDENLLPNVEGLKGPFGCLNKARYGIAWGALGAAEFCWHAARSYT
                     LERKQFGRPLAANQLIQKKLADMQTEITLGLQTVLRVGRLMDEDRASPEMVSLIKRNS
                     CGKSLDIARSARDMHGGNGVSDEYHVMRHLMNLEAVNTYEGTHDVHALILGRAQTGLQ
                     AFSG"
     gene            complement(10174..10929)
                     /locus_tag="VN496_06760"
     CDS             complement(10174..10929)
                     /locus_tag="VN496_06760"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXF16686.1"
                     /translation="MRLNNKIAIVTGGGSGFGEGIARRFVEEGCKVIVNDLKADGGQR
                     VTKALVSNGGQAKFVQGDVSKASDWDRLVKETLDTYGSLDIVVNNAGTTHRNQPLLDV
                     TEEQFDRVYSVNVKSLFHGARAVVPYFRKKGGGVLITIASTAGVRPRPGLVWYNGSKG
                     AAIVISRAMAVELAPDKIRVNVVNPVAGDTPLLAEFMGADTPEMRAKFIASIPLGRLS
                     KPIDIANACLYLASDEAEFITGACIEVDGGRCV"
     gene            complement(11025..12047)
                     /locus_tag="VN496_06765"
     CDS             complement(11025..12047)
                     /locus_tag="VN496_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXF16687.1"
                     /translation="MDSVDLQVLRSSVAWLEEGHRVILATIVETWGSSPRPAGAMLAV
                     RGDGLVAGSVSGGCVEDDLILRIRNKSLAIDRPEIASYGISKDEATRYGLPCGGKLEL
                     VLEPVNDKRALSDLLDRVDRHELTARTLDMQTGAVRLHAANRSDSTLSFDRKQLTTVH
                     GPRWRLLIIGAGQLSHYLAQMAQALDYSITVCDPRDEYADTWTVEGAVLNRGMPDDVV
                     VAMNLDSHSAVVAVTHDPKLDDLALMEALKSPAFYVGALGSRLNSQKRHERLMMFDLS
                     EAELSRLHGPIGLNIGSKTPPEIAVSILAEMTAVRHGVALDKVGVREDAERFDNSRPV
                     CVATGV"
     gene            complement(12201..>14119)
                     /locus_tag="VN496_06770"
     CDS             complement(12201..>14119)
                     /locus_tag="VN496_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXF16688.1"
                     /translation="PAEVYINALLGGQLTGGSTSVRDAWEKLRKAGASARMMLVAAAA
                     DQWGVDAGQCKAANGAITGPGGKRVNYGQVAAKAATMEVPKDVPLKPSSQFKVVSNMK
                     QRRLDTEAKVKGTAQFGIDTRVPGMLYAAVAMSPSIGGKVASYDDTRAKSMPGVKAVV
                     QYSRGVAVVADSYWQAKKAKDLLQIQWDGGPNANSDSKSIWDGIRNASDQPGAVFREH
                     GNVESGMSEAAKKVESTYQLPFLSHSPMEPMNTTADVRADKAIIITPTQFQQLIPHVV
                     GGATGLKPEQVEVHTTFLGGGFGRRVEVDYAIDAAEISKAVGAPVKMVWSREDDMTHD
                     SYRPASLMKLSAGLDASGKPVAMRFHSTSPSISARLFPSIVKDGIDPFAVEGIDNYPY
                     DTPNMKLTYQMHDAGVTPGYWRGVSHNLNAVALECFVDELANAAGKDPIQYRIDMLDM
                     GSTKHQWSGLSAGVPVGTRMKRVLEEVRAKSGWGKKLPDGRGMGVAVMEGYNTVIAMV
                     AEVSVSANYDVTVEKVTAVVDAGTLVHPDQALAQMQGTINFGQSACMWGEITIRNGGV
                     EQSNFDMYRVARINENPKVLDIHFIKSDSTPGGLGEPGTAVIQPAIGNAIFAASGKRC
                     RTLPFSPENIKGSA"
BASE COUNT         3268 a         3908 c         3797 g         3146 t
ORIGIN      
        1 aggatcgtat atacggagcc gccaattatc ttgtctattc acgcctgaag ctgaccttgg
       61 cggccgattg gaacgacacc gaccatggcg aggcatccag ggactctctg gattcgaaga
      121 tcaacaatac gacagcaacc gttaattgga ttacacccgc ggaaaatact atcggagcgc
      181 aatttcgcaa tgcaaaagcg agatatccca atagcgctat agtcggaaca tcactggttc
      241 aaaacgacta caccgaaaat gaatatagcc tggtgacata ctggaggctc acgggcgcct
      301 cagaaatacg tgcccgcctg ggccataccg accgctcatt cgagcaagcc ccaaatcggg
      361 actacagcag tccgacctgg cgcttgaatt acatttggcg accgaccgga aagactgccg
      421 tggatttctc aacttggcgg gaactctcag actttcaaaa cctcagtgca aattacgttc
      481 gcatcaccgg cattagtgtc atcccgacct ggtcgttagc gcccaaactt gcattgcgag
      541 gcaagatctc atacgagaag ctggactatc ttggcgaccc gggcctcaca gcgccagcgg
      601 ctcagcggga agacaaggac aagatatacc aggtttcctt actgtggaca ccttggcggc
      661 tgactgaatt ggacttcaac ctggagacgg gccgaagaac atcaaatcag gccctggccg
      721 actttcaata caatgccatc agctttcttg ctacccgtta tttttgagtt gcctggccgc
      781 aataacaatt tgttcaagtc attcgctaat cacttggcag aaaattagct ttttgccaac
      841 gccaggcatc tgcgcacatg gagtcaatac cgcggtttgc ccgccatccc attaccttaa
      901 aggctagggt gggatccgcg taacaggccg caacatcgcc cggacgccga tcgacaatcc
      961 gatagttgat ggtgcgacca cttgcctctg aaaatgcttt tatcatctcc agcacgctgt
     1021 agccccggcc agttccaaga tttagggtca acaaatcggc ttgctcagca agaacattaa
     1081 gagccgccag gtgcccttgg gccagatcga caacatggat atagtcccga accccggtcc
     1141 catcgggcgt cggatagtca gcgccgtaga cggaaagtcg cggcagaata cccgctgcca
     1201 cttgcgcaac ataaggcatc agattattcg gtgtaccacg gggctgctct ccaatcagcc
     1261 cagacgcatg cgcgccgacc ggattgaaat aacgcaacaa cgcaacgcgc cactgcgaat
     1321 cggcccttgc ggtatcgcgg aggatatctt caataatcag tttggagcgg ccatacggat
     1381 tagttgcgct tagcggaaaa tcttcagtaa tcgggaccgc gtgcggattt ccatacacgg
     1441 tcgctgaaga agaaaatacc agagtcttca cccccgcctc gtccatggct tctagcagaa
     1501 acaacgtgcc cgtgacgttg ttgtcgtaat acgcgagagg gttgcttacc gattcgccca
     1561 ccgctttcag gccggcaaaa tgcagtacgg cgtggaacga atggtccgaa aataatttcc
     1621 gcagtccgcc gagatcacgc atatcaagat tagcgaagcc gggcctgcgt ccggaaatgg
     1681 tttcaatccg gtcaattacc tcagacgtac tgttgcacaa attgtcggca ataaacacct
     1741 catgtcctgc tgcctgcaat tcaacaacgg tatgtgagcc gatatacccg gcgccgcctg
     1801 taaccaagat ggattgcgac gcagtcatca gcggtattct cggtcagtaa ttgccatggc
     1861 taatctctcc actcggttgc ttgcatatgt tgcttcacgc cgaattatct cgcgaaagcc
     1921 tgattaaatc ttttacgaat gtcgcgcggt cgccggactt ccgtaattat gaagtcacca
     1981 cactgaaggt gaacgctcaa caaagtcgaa gccgtcctcc attcggctca gtactgatct
     2041 cgattctcga ctatcgaatc gcggggattc tcgacccccg ttacgtacat taagctttgc
     2101 tcaactaaca agggacctta gaatctaccc cggtccaaga cggcctcagt agcttttaat
     2161 gcctttagcg agatccgata attgaaactc cttatcaaac aagtcgacaa cgacgggcgc
     2221 cttgccctca ttcctgaata tgtcaagacc agttggacga ttgacgtcgt gaaccatgag
     2281 gatcgcaatg aactaaaagg caagcttttg gatgcggacg ctgcgatctc gatgaactgg
     2341 ccgcgagaca tgccgccggc accgaagtta agactgctgc aattacctgg agcgggtacc
     2401 gacgatatca tcttcgatgc cgttcccgca caagtcgcgg tgtgtaatgt cttcgagcat
     2461 gaaatcggga tcgcggaata tgttctttct gcgatgttgc agttgacaat cggaatcccg
     2521 cgcatggatg cagccatcag gcgcggggaa tggtatggct ctcatttgac cggtccacgg
     2581 cacggcgagt tgtacaacca aacgctcgca atcgttggtt acggccgcat cggccacgag
     2641 gtcgccaaac gggcgcgcgc gtttggcatg aatgttttgg catgcagccg cacgccaagg
     2701 gcgggtgacg gttttgtgga cgaagtggga tccatgcagc aactggatac gctactgggc
     2761 aaagctgatt ttattgtgct cgcagtgccg ttggatgcat ccacgttaaa tttgatcgat
     2821 gaaagcagga ttgccaaaat gaagcccagc gcgtttattg tgaatgtcgc gcgtggtgcc
     2881 ctggtcaatg aaaaggcact ttacgaagcg tgcaagcatc gtcgaatagg aggtgcagca
     2941 atcgacactt ggtatcgcta ccctacagat ggtaaggtga gtggcgatcc gtccaatatg
     3001 ccgtttcgcg aacttgagaa tgtcattatg acgccgcatg gttctggctg gacacatgcc
     3061 ttgttgccgc gccgatgccg cttaattgct caaaaccttg accggctcgc acgcggcgaa
     3121 ccactgctca atgtggtgag ggccgtggcg gttgcacatt gacgcacgca aattacaact
     3181 tggacgacca tcttatcgaa tccgctgcca agaccctttg gtctacatgg caggccaatc
     3241 agcacataga cgccctgccg atagcgtgcc ggccgtcaac gcgtattgat ggataccgga
     3301 ttcaagccca tattgcccgg ttatcagcgc aaaaggtctt cggatggaaa attgccgcaa
     3361 caagcacggc tggacaaaaa catatcggcg ttgacgggcc gcttgctggg cgcttgctgg
     3421 aaaaacgagt attcgcgaca ggtgcaacga tctcattgga taacaacgtc atgaatgtgg
     3481 cagaggcgga attcgcgttc cgcatcggaa aagatctccc gcccaaaaag ggcacttact
     3541 cgcgtgaaca agtgttcgct gctgtggaaa gcatgcatcc cgcgatagaa attccagatt
     3601 caagatatcg ggattacgtt acggctggtg cagcgcaact gatcgcggat aacgcgtgcg
     3661 cgagctattt tgttctcgga cccgcggtaa ccttcaagga ctggcgaggg catgacctgg
     3721 tacgccatcc agtcacggtt tacgttaatg gcaatttggc gcggcaaggg actggtgaga
     3781 atgtgcttgg agatccgcgc atcgcgctga cttggctcgc aaacgaactg tcgctcgtcg
     3841 gcgacacgct gcgtgagggg caaacggtga ccactggcac ctgcatcact ccggccgccg
     3901 ttgtcggcgg cgatacggtt aacgccgatt tttttgaatt tgggcgtgtt gaggcgaaat
     3961 tggcgcgctg atcttgcacg tgaagcgttg cccaggaaaa gtggctggtg tggcggcgtt
     4021 tttgtttaca tgcgatagtg gcctttgatc cggcacggaa gaaagtcgcc gctgatcccg
     4081 gtgcgtcggc tgatttggct acggcatcat gaaatgagtt tccttttatg cagctctatt
     4141 atcctcgctc gtttctaaag ctgatccttg cgggcttttc gctcgccgtt ctgccattga
     4201 tatttgctct gatcaataac gcctattcga tccaccaact tgcaacacac agccagcgcg
     4261 ccgtgtatca ggctgtccag actacgcaga gcacgcgttt tttgattgaa caaatcacca
     4321 gcatggagcg aagcattcga cagtttgcga ttatcggaga tccgtcgttg ttgcaaggct
     4381 acggccttgc gcacgagcgc tttgccgaca ctgcccgcaa aatgactgcg cttcccctgg
     4441 acgacaagca aaaaatagca atgcaggcac tgaggaaccg cgaaacggct ttattcgagc
     4501 gcatcggaaa atctggcgcc aagtctgaaa ctgtgaacgt tgccgcgacg gaactcgcag
     4561 cactatcaga agctgcacgt cgaatagatg aacaaggcaa ttcgctggtg gatcgcgaag
     4621 tcgataccat gcagaaaatg gccggcgaag tgcagcgctt catctattgg caattgctgg
     4681 cgctggtccc gatcgcgtta ctattggtga ttggggccac aatgctgatt tccaaaccga
     4741 tcgcgcagct cgagtccgcg atacggagtc tgggggaggg aagttttgga gaacgcattg
     4801 cagtcagcgg gcctgccgat ctggaatccc tgggaagaca actcgactgg ctgcgtttgc
     4861 ggttgctgga cctggaagag cagaaatcca gatttttgcg gcatgtatcg catgaactga
     4921 agactcccct gacggcatta cgtgagggcg ttgaactgct ttccgatgaa gtcaccggca
     4981 aaatgacggc cggccagttt gaaatcgctc gcatcttgag gcacaacaca ttgcggttgc
     5041 agaaactcat tgaagacttg ctcagctacc atagtgcaca gttccacact tccgctctgt
     5101 cgttttcgca attcaacttg aaaggactta ctgcgcgcgt cgcacagcaa cattcccttg
     5161 caatgcgctc gaaaaacctc agtctgaatc ttgccgcgtc cgaactcaac gtcgaagccg
     5221 acgaaaacaa gttggaagca atcgtggata atctgctctc taacgctatc aaattttccc
     5281 ctgccggagg aacagtgagc gtccagctgc gcaggcgcgg attggatgtc gttatggatg
     5341 tcgtggatca aggtcccggc attctcccgc aggataggga ccgggtattt gaagccttct
     5401 atcaaggcgg gtctgaatat accggacccg taaaaggcac tggactggga ttagctatcg
     5461 tcaaggaata cgtaagcgcg catcatggca gcgtggaaat cattgtcgat ggcaatccag
     5521 gcgctcacct gcgggtaata cttccatcac gccaggtcga ggcggcggca taatgtggcg
     5581 aacgggcagc gttgtgcttg tcgcaataat tgccggttgc gctccagtgg acagcagaac
     5641 tacgcgtccg gagacgccgt ggtatcgctc ggtcacggaa ccggcacaca gtgacgcgga
     5701 ccgcataata caattctatg atcgtcttac cgccatgaag ggaggtgaat tgacacacga
     5761 atttgaaaca gtccgtgaag cttttgagaa tgacaaatca caactgaatc gtatgaaact
     5821 cgcgttgctg ctttcctatc caggctccag ttttcgtgac gacaatgcgg cgattacgct
     5881 gcttggtcct tttataaagg aaaaggcgca agaaacctcg tccttgcgtc cgctggccgt
     5941 gtggctcaat tctgaactgc ttgaggtgcg gcgcgcggaa gaagcctcgc aacaatacgt
     6001 atcaaaatta aaagacgaac agcgccgggc agaagccttg caacagaaaa tcgagacctc
     6061 tcaacaaaaa gcagaggccc tgcaacagaa gcttgacgca attctggaaa tggaaatgaa
     6121 aatgatagaa cgagaacaga gtatcccgaa gaaaaaatga tcatgtcaaa ggcccgcatt
     6181 cttatcgtcg acgacgatcc cgatctattg cgccttctta ccttccggct tgaaggtgcg
     6241 gactatgctg tggaaagtgc agacagcgct gagagagcac ttgcccggtt ctcagtcgca
     6301 atgccgcagc tcgtaataac cgatttgcgc atgagcggca tggatggcat ggcgcttttt
     6361 gaaaatatcc accgaagcaa tccaacgctg cccgtaatta ttctaactgc gcacgggacc
     6421 attccggacg cggtagcggc gactcaacgc ggtgtttttg gctatttgac caaaccgttc
     6481 gatagcaagg acctgctgca acaagtggag aaagcagtcg ctgtaagcgg cggtcgcaat
     6541 gaaaaaatga acggggaagc ggattggcgc gtcgagatca tcacgcgtag cctggccatc
     6601 gaaagcattc tcggtaaagc gaaactagtg gcggctagcg atgctagcgt aatggttcga
     6661 ggcgagagcg gtacaggcaa ggaacttctg gcccgcgcga tacaccgggc cagttcgcgc
     6721 gccgagaggc catttgtcgc cataaactgc ggcgccattc cggagcagtt actggaatca
     6781 gaattgtttg gacatatgaa aggttcgttt accggtgcgg ctcgcgacta caagggactg
     6841 tttcagtctg cggaaggcgg cactgtattt ctggatgaaa tcggagacat gccgttaccg
     6901 cttcaagtaa aactgctgcg tgttttgcag gacagggaga tacgccctat tggaacgacg
     6961 caaaccattc cggtagacgt ccgcatcatt tctgctactc accggaacct tgaggaagca
     7021 atcaaaggag gaacatttcg cgaggatctc tattaccgct tgaacgtcgt ttcgtttgct
     7081 ttgccggcgc tttgcgaacg gcgcgaggat atcccggtcc tggctacaca tttcctccac
     7141 caacttgcca gccgctataa aaagacgctt aacggcttcg catctgaagc catggaaatc
     7201 ctcgttaaat cggcttggcc agggaatgtc cgcgaactgt ataacgtcgt cgagcaggcc
     7261 gtggcgctgt cgaccacccc aataattacc cccgctctgg ttgatagcgc aatgcgcggc
     7321 cagatcgccg aactgtcgtc gtttgagtcc gctcgaagcg agttcgagcg ggattacctc
     7381 gccaaactgc tcaaaataac gggcggcaac gttacgcaag cagcaaagct tgcgaagcgg
     7441 aatcgcacgg aattttataa attgctgcag cgccaccacc tcgaccccaa gcttttcaag
     7501 gcggcctaaa agaacgccaa gtcctaccgc ttcctcggtc ttattactta taggactcag
     7561 ggattgtctc tatatgacga catgtgtcgg gcaaaagcga cttataaaaa tacctaatct
     7621 attgttttgg taatagatta cagcttgggc atgagtactg cttatagctt tgcaacagtg
     7681 tttttttatg ccctcgtagt gtggggacaa attcaacggc ggtgccatga ctaagaccga
     7741 aagccctgca ttaacgcccc tgcttgtcgc agctaccatc tcagttctgg cttttagcgg
     7801 aatcggtgta gctgctatca ccggtcacct ctccataacc cgctcaagtc tgaatccgtt
     7861 ttccagcttc agcaactcag cggctagcgg cctcattcaa gcccccgttg tggcgactgc
     7921 cgctcatcag ggacttaccc gccatcgcgg agaaacggtg gtagatggca aaccggtcaa
     7981 ttacaaattc ggggcacggg taccttctcg caagattgtt tgcccggatt gcggggtagt
     8041 ggattccatt aaaccgggtc aggcgcgacc cgtcggcact ggattcactc gttcccaggg
     8101 gcttgagagt gaaggcgcgg caattctgaa ggtcggaggt cagtctggct acgatccagt
     8161 cgccagcggc ggagcaggtt tcgtcgtaac cgttcacatg gagaacggta ccgtgcgtac
     8221 gatttatgag ccggagcggc cctcgttcag catcggcgaa agaataaagc tggtaaatgg
     8281 gtcagtaatc cggattggct gatcaacaca caaacaacac ctgccagagg cactccaccc
     8341 gcccgcacta ataagacgaa agatttcccg tttttctgcg tgttcacgcc cgatttcctc
     8401 ccttatcggg cgtttttttt cgcggctgac tgccagacca tcaaaggcgc cggcgggtag
     8461 aattcaaact gtaagagctt gtcccaccct ttcccgactc cagcagggca cagaaactac
     8521 agattcttcc agccatgagg aaacttctgc tgctcttggc agctggactg ctcgccgcct
     8581 gcgctccggc caaaaaagag gccaacgtca atttggccgg ctattcgcct gcgttccgag
     8641 ccggttatgg cgatggatgc gaaagtagca agcgaaccgc cggccaaacc cgggacgagg
     8701 accggttcaa gcacgatgcc caatatgccc tgggctggcg tgacggtttc gacatctgcg
     8761 gacggaagaa aaagtagcaa ccaggactga taagctgcct cgctgcgatc cgtagcgctc
     8821 ccaaccctgc accatcccgc tcctgacttc ggtgttcatg acttaagata ctgaggctgc
     8881 gcggccttct aaagcgtcac gatcaggaga attgcatgaa tgcccccctt gcccgccctg
     8941 ctttccattg ggatgatccc ttgctccttg aagagcagtt gaacgatgac gaacgcatgg
     9001 ttcgcgaaac tgctcgcgcg tactgccagg acaaactcat gccgcgcgtt gtatcggcgc
     9061 atcggcagga acgtttcgat agagaaatct tcagcgaaat ggggaatctt ggctttctcg
     9121 gttcaacctt ggaaggctat gggtgcgccg gcgtcaacca cgtctgctac ggattgatcg
     9181 cccgggaaat cgaacgcgtg gattccgggt atcgctccat gatgagcgtg cagtcgagcc
     9241 tcacgatgta tcctatttac cgctatggct cggagagcca gcgccagaaa tatctaccta
     9301 agttggcatc gggcgaatct atcggggcat ttgggctcac cgagcccgac ttcggatccg
     9361 atgccggaag catgatcagc cgcgcgcgca aagtggatgg cggcttcgtg ctgaagggtg
     9421 cgaagatgtg gatcacgaat tctccagtgg cagacgtctt ggtcgtatgg gcgaaagacg
     9481 atcgcggcat gattcggggt tttattctcg aacgcggaat gaagggcctt gccacgccta
     9541 aaattgaagg caagttcagc ttgcgcactt ccatcactgg cgaaattgtc atggatgacg
     9601 tttttgtccc ggacgagaat ctattgccga atgtggaagg actcaaaggc ccgttcggat
     9661 gtcttaacaa agcgcgttat ggcattgcat ggggcgcgct gggagctgct gaattctgtt
     9721 ggcatgccgc gcggagctat acgctggaaa gaaaacaatt tggcaggccg ctggctgcga
     9781 accagttgat ccagaaaaag ctggctgaca tgcaaaccga aataacgctt ggcctgcaaa
     9841 cggttcttag ggtaggtcgg ctgatggacg aagaccgcgc gtccccggaa atggtatcgt
     9901 taatcaagcg caattcctgt ggcaaatcgc tcgatatcgc ccgttccgcg cgcgacatgc
     9961 atggtggtaa cggagtgtcc gacgaatacc acgtgatgcg ccatctgatg aatctggaag
    10021 cagtgaacac ctatgaaggt acgcacgatg tgcatgcgtt gattctgggt cgcgcccaga
    10081 cgggcttaca ggcattcagc ggttagcgct ttgccagtct gttcggcgcg ggactaaaac
    10141 gagccgctca atgccctggc cgtcgcctgc ttgtcatacg catcgaccgc catccacttc
    10201 gatgcaggcg ccggtaataa actcagcctc gtcggaggcc agataaagac aagcattggc
    10261 aatatcgatg ggcttgctca agcgccccaa tggaatgctc gcaataaatt tggcccgcat
    10321 ttccggcgtg tcagccccca tgaattccgc cagcaaaggt gtatcgccag ccaccggatt
    10381 gactacattg acccggattt tgtccggtgc gagttcaacc gccatggcgc ggctgatgac
    10441 gattgcggct cccttactgc cgttgtacca aaccagaccg ggtcgcggcc gcacgcccgc
    10501 ggtcgacgca attgtgatca gtacgccgcc gccttttttg cgaaagtaag gcacgacggc
    10561 gcgggcgcca tgaaataagc ttttaacgtt cacggaatag acgcgatcga actgttcctc
    10621 ggtcacatcc aaaagcggct gattgcggtg cgttgtaccg gcattgttga cgacgatgtc
    10681 gaggcttcca taggtgtcca gcgtctcttt taccaatcga tcccaatcgg aggctttgga
    10741 gacgtctccc tggacgaact ttgcctgacc accattagac acaagagctt tggtgacgcg
    10801 ctgacctccg tcggccttca ggtcgtttac gataaccttg catccctctt cgacgaagcg
    10861 tcgggcgatg ccctctccaa agccggaccc cccgccggtc acaatcgcaa ttttattgtt
    10921 aagacgcatt ttttcttgct cctggtaatt tattcatcac ggggatcgca caaaaaatat
    10981 gggagccacc gtggctccca ttgccaaagt tcattgcatt gagctcagac accggttgca
    11041 acgcagacgg gccgggaatt gtcaaagcgt tcggcatctt cccgtacacc aaccttatca
    11101 agcgccacac cgtgacgaac ggctgtcatt tcggccagaa tcgacacggc tatttcaggt
    11161 ggcgttttgc tgccaatgtt caaaccaatc ggtccgtgca agcggctcaa ttccgcctcc
    11221 gacagatcaa acatcatcag gcgctcatgc cgcttctggg aattaagacg cgacccaagg
    11281 gctccaacat agaatgccgg cgactttagc gcctccatca gcgcgagatc gtccagcttc
    11341 ggatcatgcg tcaccgccac gacggcactg tgtgaatcca gattcattgc aactacaacg
    11401 tcatcaggca tgccccggtt caggacggct ccctcgacag tccaggtatc ggcatactca
    11461 tcgcgcggat cgcataccgt aatgctgtag tccagggcct gcgccatctg tgccagataa
    11521 tgcgagagct gacccgctcc gatgataagc agtcgccacc tcggtccgtg cacggttgtt
    11581 agttgtttgc gatcgaagct tagtgtgctg tcgcttcggt tggcagcatg tagcctcact
    11641 gctccggtct gcatatccaa agtccgcgca gtcagctcat ggcgatccac ccgatcaagc
    11701 aggtcactga gggcgcgctt gtcgtttaca ggttcaagca ccagttccaa cttcccgccg
    11761 cagggtaggc catagcgcgt cgcttcatcc ttgctgatgc cataagacgc aatttccggc
    11821 cggtcgatcg ccaacgactt gttgcgtatc ctcaatataa gatcgtcttc aacgcaaccg
    11881 ccagagacgg aaccagcaac cagtccatca ccacgcaccg cgagcattgc accagccggt
    11941 cgcggcgaag atccccacgt ttccacgata gtcgcaagaa tgacgcgatg gccttcctcg
    12001 agccaggcga cactgctgcg caaaacttgt aaatcaacgc tgtccataag ccctgcctta
    12061 tattcgctac cgttctgtct gaacgggaac aacgatttta atcccagcag accggccatt
    12121 gccaataaaa aaccccccga cgagtcgccg aggggttaag gaactgctgt ttgcccggtt
    12181 gcccgggctg actaggtctg ctaggccgag cctttgatgt tttccggtga gaacggcaaa
    12241 gtacggcacc gcttgccact ggcggcaaaa atcgcattgc cgatggctgg ttgaatgact
    12301 gcggtgcccg gttcgcccaa tccaccgggt gtgctgtcgc tcttgatgaa gtgaatatcc
    12361 aggaccttcg gattttcatt tatgcgtgcg acacggtaca tgtcgaagtt gctctgttcg
    12421 actccgccgt tgcgaatcgt gatttcaccc cacatgcaag ccgattgacc gaaattgatc
    12481 gtgccctgca tctgcgcgag agcctgatcg ggatgcacca gagtcccggc gtctacaacg
    12541 gccgtgactt tctcgaccgt cacatcgtag ttggcggaca cgcttacttc ggcgaccatc
    12601 gcgataacgg tgttataacc ttccatcacc gccacgccca taccccggcc atcgggtaat
    12661 ttcttgcccc aaccggattt cgcgcggact tcttccagaa cccgcttcat gcgagtgccg
    12721 accggcacgc cagcagacag gcccgaccac tgatgtttgg tcgatcccat gtccagcatg
    12781 tcgatgcgat actggatcgg atctttgccg gctgcattag ccagttcatc gacgaagcac
    12841 tcgagcgcga ctgcattcag attgtgcgac accccgcgcc aataacccgg cgttacccca
    12901 gcatcgtgca tctggtaggt gagcttcatg ttcggagtat cgtagggata gttatcgatg
    12961 ccctctacgg caaacggatc gatgccatcc ttgacgatac ttgggaacag acgcgccgat
    13021 atcgacggac tggttgagtg aaagcgcatg gcgaccggct tgccgcttgc atccaaacca
    13081 gctgacagct tcatcaggct ggccggccga tacgaatcgt gtgtcatgtc gtcttcgcgg
    13141 ctccacacca tctttacagg ggcacccaca gccttcgata tttcagcggc atcgattgca
    13201 taatcgactt ccacccggcg gccaaatcca ccgccaagga aagtggtatg tacttcgact
    13261 tgctcgggct tcaatccggt tgctccacca acaacgtgcg ggatcagttg ctggaactgg
    13321 gtgggggtga taatgatcgc cttatcggcc cggacgtcag ccgtcgtatt catcggctcc
    13381 atcggggagt gcgacaggaa tggcagttga taagtggatt cgaccttttt ggctgcctcg
    13441 ctcattccgg actcgacatt gccatgctcc cggaacaccg cacctggctg atcgctggca
    13501 ttgcgaatgc cgtcccagat cgacttcgaa tcgctgttcg cgtttggacc accgtcccat
    13561 tggatctgca acaggtcttt ggcctttttc gcctgccagt aggaatcggc aaccactgcc
    13621 acgccgcggc tgtattgaac gacagctttg acgcccggca tggatttggc acgggtgtca
    13681 tcgtaactcg caaccttgcc gccgatgctc ggcgacatgg cgaccgcggc gtaaagcatg
    13741 ccgggcacac gagtgtcgat gccaaattgc gcggtgccct tgacctttgc ttccgtgtcc
    13801 aaacgccttt gcttcatgtt gctgacgact ttgaactggc tggacggctt cagcggaacg
    13861 tctttcggca cttccatggt ggctgctttg gcggcgacct gaccatagtt gacgcgtttc
    13921 ccaccggggc cggtgattgc gccgttggcg gctttgcatt gtccggcatc aacgccccat
    13981 tggtctgcgg cagcggccac gagcatcatg cgggccgatg ccccggcttt ccgaagtttt
    14041 tcccaagcat cgcgaacaga cgtcgatccg ccggtgagct gaccgccgag caacgcgttg
    14101 atgtaaacct cggccggtg
//
LOCUS       DATMZQ010000208         6069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_772272, whole genome shotgun sequence.
ACCESSION   DATMZQ010000208 DATMZQ010000000
VERSION     DATMZQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772272"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VN496_06775"
     CDS             complement(<1..360)
                     /locus_tag="VN496_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXF16689.1"
                     /translation="MDAWLFWKALSTGLAVAAPVGPMSLLCIQRTLNHGRAGGLVFGA
                     GIAAADMTYAALAGFGITAISSLLLAGSFWIRLAGSSVLIALGVRVALSVPATDHHAK
                     VTQSPGRSFVTAYGLTLA"
     gene            complement(506..1129)
                     /locus_tag="VN496_06780"
     CDS             complement(506..1129)
                     /locus_tag="VN496_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16690.1"
                     /translation="MVRLLIMCLLSLLPLTVLAAAEDTMLYIEYKLTGKQVAADSFDA
                     QTKKVWRVGMNYLRFEDAPNPQTKVHGLIIVAEPDIWVVDLNTNQAQHTVDPGPHYSI
                     HFPLLASEPSPKLKALEFGHELEYFQQNGAETLPAEQIDGVKCKPLRLQIDGREVTLY
                     LRTDDTPFQVSVKSAQYEYAIRFLRYESNRKIDKSLFQLPLGVQLQN"
     gene            complement(1197..2330)
                     /locus_tag="VN496_06785"
     CDS             complement(1197..2330)
                     /locus_tag="VN496_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXF16691.1"
                     /translation="MISCSAHLARLLLTLLLIALGGCAHAARSGCSGDIPAIFDRVSP
                     AVVMISAQSINPYRVQGRVTRIIGSGFIYDAKGLIITNSHVAFGRQTLYVTLDDGNVV
                     PARLLGADPIYDIAVLQISKVSKGKLATVPLGDSEHVRVGESVIAIGNPFGLDQTVTR
                     GIISGVNRILPETPLSLSEPLIQTDTPINPGNSGGPLLNLCGEVIGMNTAIVPEAQSI
                     GFAIPINLVKSVLPTLIKEGRFIRPWLGFQGTVIGPEVRKVLKLPLTDGLLIEVIEPG
                     SPAESVGLRGGEIEFTLGTLSLLLGGDIITRINGVPTNDSERVAPIMRGLKVGDTLSM
                     SVFRDGETREVSYTLPERPLLPGDLPEEQPGPPPMMAPRRDKP"
     gene            complement(2349..3134)
                     /locus_tag="VN496_06790"
     CDS             complement(2349..3134)
                     /locus_tag="VN496_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16692.1"
                     /translation="MPDKLFSVADQVVLVAGASRGIGRALAEGFAERDARVMIAGREE
                     RTLARTAQEISRGKHAVGFEVCDVAKPEEVAQLVKKTIGRYGHIDTLVSVAGVNKRMK
                     AENYAIEEYDWIININQRGAFIVAQAVGRHMIERKAGAIINVDSLNTYAPLTGVAPYA
                     MSKAGVLMMTRALANEWGKHGVRVNSIAPGFFPTALSKTMWEQEKMIHWAKTNTPLGK
                     LGDVQELVGAAIYLASPASAFVTGQTIRVDGGFTAGISWPIDL"
     gene            complement(3229..3990)
                     /locus_tag="VN496_06795"
     CDS             complement(3229..3990)
                     /locus_tag="VN496_06795"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXF16693.1"
                     /translation="MKTIDLNCDMGEAFGAWTMGDDDTLLDHVTSANIACGYHAGDPH
                     TMHRTVEIALKKGVAIGAHPSLPDLQGFGRRSMKISAEETYDMVMYQMGALSAFARAC
                     GGKLAHVKAHGALYNMAAKDATIARAIARAVRDFDSQLVLFGLAGSEQIRAGEEAGLK
                     TASEVFADRTYQSNGSLTPRNQPNAMVRDVQTSLMRVKRMVEEGKVESNEGADLIVKA
                     DTLCIHGDEPGAVEFASKIRRMLADAGIRVTPVVP"
     gene            complement(4101..5108)
                     /locus_tag="VN496_06800"
     CDS             complement(4101..5108)
                     /locus_tag="VN496_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXF16694.1"
                     /translation="MSLTVLQPGMLTTVQDCGRTGFQHLGVPVSGAMDFFSHRIANIL
                     AANDTREATLEITLRGPRLRFEKDALIALCGAELSPTLDGIPAPEGKPVRVRAGTVLD
                     FGRALAGCRCYLAVHGGFDVPVVMGSRSTYDTARLGGLDGRALRKADVLPLGAAHGAN
                     TYPGLEQQLVDSGHSFSATKWAVNQHIEQLRRAPQIVRILAGRHWDAFPSTAREALTS
                     GEFCVAPDSNRMGCRLESEHLVTGGPGEILSEAVTFGTIQVPPSGKPIVLMADRQTVG
                     GYPKIAEVATVDLHLLAQLRPGDRLRFELTSLAQAQALWLKREREIITIREAVEPRIA
                     Q"
     gene            complement(5105..5812)
                     /gene="pxpB"
                     /locus_tag="VN496_06805"
     CDS             complement(5105..5812)
                     /gene="pxpB"
                     /locus_tag="VN496_06805"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HXF16695.1"
                     /translation="MLAAPRFFPMGDQALVVEFGERIDITLSAHIAALAQRLRDSRPI
                     GVLDIVPAYATLALHYDPAAMGSGASPYETLCDIIASWLETQATAIEAAGRTIEIPVC
                     YGNAFGPDLDAVARSVGLSSGEVIAIHSGSTYYVHMLGFVPGFAYLGGLDNRIATPRR
                     DTPRAHVPAGSVAIAGEQTAVYPLDTPGGWQLIGRTPLQLFKPDSSPPSLLNAGDQVR
                     FMQISAVEFEAQSRAAP"
     gene            complement(5842..>6069)
                     /locus_tag="VN496_06810"
     CDS             complement(5842..>6069)
                     /locus_tag="VN496_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXF16696.1"
                     /translation="ENASQQRFFTGFSGWAAGQLEDEIAQGGWYVLAFDSKIVFDMNP
                     LTLYDEMLKRATAPRIEAAGDAGRLSSAPAR"
BASE COUNT         1185 a         1838 c         1791 g         1255 t
ORIGIN      
        1 ggccagcgtt aggccatacg ccgtcacgaa tgaacgcccc ggagactgcg tcacctttgc
       61 atgatgatca gtggcgggta cgctcagagc gacgcgcaca ccgagcgcta tcagcactga
      121 tgatccagcg agcctgatcc agaaagagcc agccaggagc agcgaggata tcgccgtgat
      181 tccaaaaccg gccagtgctg cataagtcat gtccgctgcc gcgatcccgg caccgaacac
      241 gagtccaccc gctcttccat gattcagcgt gcgctggatg cacagcaggc tcatcggtcc
      301 caccggtgcg gccactgcca atccggtgct caatgccttc cagaatagcc atgcatccat
      361 aaggtcgctg cttcagcttt gccgtgcacg catacggaaa gacgtcacga ttgtccaggc
      421 catgcttcgg aatgcgtgcg cgcagcgcaa tctaaaagcg ttccgagtgc ctgactcgaa
      481 acgcgctgcc acccggcgta cccgcttaat tctgcagctg aacgcccagc ggcagctgaa
      541 acagcgactt gtcgattttg cgattggatt cgtagcgcag gaagcgtatc gcgtattcgt
      601 attgcgcaga tttcaccgaa acctgaaatg gagtgtcgtc cgtcctgagg taaagcgtga
      661 cttcgcggcc atcgatttgc aaccggagcg gtttgcattt gaccccatcg atttgttcgg
      721 ccggcaaggt ctccgctcca ttctgctgga aatactccag ctcatgtcca aactcgagcg
      781 ccttcagttt cggcgaaggt tccgaggcca gcaacggaaa gtgaatgctg taatggggcc
      841 ccgggtcgac ggtgtgctgc gcctgattgg tattgaggtc cacgacccag atgtcgggtt
      901 ccgcgacgat aatgagtccg tgaacctttg tctgtggatt tggcgcatcc tcgaagcgca
      961 gataattcat gccgacgcgc cagactttct tggtttgcgc atcgaaactg tccgccgcaa
     1021 cttgtttgcc ggtcagcttg tattcgatgt acagcatggt gtcctcggct gctgccaata
     1081 ccgtcagcgg tagcagggac aacagacaca tgatcagcag tcgtaccatg gtggaggcaa
     1141 tttcagcgga ttggcgagtt tacaacggat ggtgcaagca tggctcgcgg cgggcgctag
     1201 ggtttatcgc gccgcggagc catcattggc gggggtcctg gctgctcctc cggcagatcg
     1261 cccggcagta atggccgttc cggcagcgtg tagctcactt ctcgcgtttc gccgtcgcgg
     1321 aacacagaca tcgatagcgt atcgccaacc ttgaggccgc gcatgatcgg cgccacgcgt
     1381 tcagaatcat tggtcgggac cccgttgatg cgggtaatga tatcgccgcc cagcagaagg
     1441 gataatgtac ccagcgtgaa ttcgatttct ccgccgcgca agccaaccga ctcggcggga
     1501 cttcctggct caataacttc gatcagcagc ccgtctgtca acggtagttt tagcactttg
     1561 cgcacttccg gtccgatcac ggtgccctgg aatcccagcc agggccggat aaaacgtccc
     1621 tccttgatca acgtgggcag gacagacttc accagattga tcgggatagc gaacccgatg
     1681 ctttgcgctt ccggcacgat ggccgtgttc atgccgatga cttcgccaca caggttgagt
     1741 agcggaccgc cggaattgcc gggattgatc ggtgtatcgg tctgaatgag tggttcgctc
     1801 aatgacaaag gcgtttcagg aaggatgcgg ttcacgccgc tgatgatgcc gcgcgtcacg
     1861 gtctggtcca gaccaaacgg attgcctatc gcaatcacgc tttcaccaac gcgcacgtgc
     1921 tcggagtcgc caagcggtac cgtggccagt ttgcctttcg acactttcga aatctgcagc
     1981 accgcgatgt cgtaaatggg atccgcgcca agcaggcgag cgggcacaac attgccgtcg
     2041 tccagtgtca cgtacagcgt ctggcggccg aaggccacgt gggagttggt gatgatgagg
     2101 cctttggcat cgtagatgaa tcccgagccg atgatgcgag taacccggcc ctggacccgg
     2161 tacgggttga tgctttgcgc agaaatcatt actacggcag gcgagacccg atcgaatatc
     2221 gctggaatat caccgctgca tccggaacgc gcggcatggg cgcaaccgcc gagtgcaatc
     2281 aaaagcagcg tgagcagcaa acgggccaga tgggcggagc aggaaatcat gtgatgtgcg
     2341 ttcgcggtct aaagatcaat gggccagctg ataccggccg tgaagccccc atccacccgg
     2401 atcgtttggc cggtcacgaa agccgacgca ggtgaagcaa gatagatggc ggcgcccacc
     2461 agctcctgca cgtcaccgag cttgccgagc ggcgtgttgg tcttcgccca gtggatcatc
     2521 ttttcctgtt cccacattgt cttcgatagc gcggttggaa agaagcccgg cgcgattgaa
     2581 ttcacgcgaa cgccgtgttt gccccattcg ttcgcaagcg cgcgcgtcat catgagcacg
     2641 ccggccttgc tcattgcata aggcgccacg ccggtcaacg gcgcataggt attcagggag
     2701 tccacgttga tgatggctcc ggctttgcgt tcgatcatgt gccggccgac tgcctgcgcc
     2761 acgatgaagg cgccgcgctg gttgatattg atgatccagt cgtattcctc gattgcgtag
     2821 ttctcggcct tcatgcgctt gttgacaccc gctacgctga ctagggtgtc gatgtggccg
     2881 tagcggccga tggtcttctt caccagctgc gcaacctctt ccggtttggc gacatcgcag
     2941 acctcgaagc cgaccgcatg cttgcctctg gatatttcct gcgcagtgcg agccaaagtt
     3001 cgttcttcgc gtcccgcaat catgaccctg gcatcgcgtt cggcaaatcc ctcagccagc
     3061 gcccggccaa tgccgcgact tgcccccgcc accaggacta cctggtctgc caccgaaaac
     3121 aacttgtccg gcattgccgt gactccttat tgttgtaaag aaactatggt tgcatccgga
     3181 cggcacgtcg tggcatgcca cacttcctgc tcaagtttcc ggctgctatc acggcaccac
     3241 tggcgtaacg cggatgccgg cgtcggccag catccggcgt attttgcttg cgaattcgac
     3301 tgcacccggc tcatcgccgt ggatacaaag tgtatcggct tttacgatga gatccgctcc
     3361 ctcgttgctt tccactttgc cctcttcgac catgcgcttc acgcgcatga gggaggtttg
     3421 aacgtcgcgg accattgcat tcggttggtt acggggcgtc aggctgccat tcgattgata
     3481 agtgcgatcg gcaaatactt cgctggcggt cttcaggccg gcttcttcgc cagcccggat
     3541 ctgttcgctt cccgcaaggc cgaagagcac cagttgcgaa tcgaagtccc gcacggcgcg
     3601 tgcaatggct cgggcgatcg tggcgtcttt cgccgccatg ttgtaaaggg cgccatgcgc
     3661 cttgacatgt gccagctttc ccccgcaggc cctggcgaat gccgacagcg cgcccatctg
     3721 gtacatgacc atatcgtagg tttcttcggc tgatattttc atgctgcgcc ggccgaaacc
     3781 ttgcaggtcg ggcagcgacg gatgcgcgcc gatcgcgacg cctttcttca atgcgatttc
     3841 caccgtgcga tgcatggtat gcggatcacc cgcatggtaa ccgcacgcga tattggcgga
     3901 cgtcacgtga tcgagcaggg tgtcgtcgtc ccccatggtc caggcgccaa aggcttcgcc
     3961 catgtcgcag ttaagatcga tcgttttcat tccgggtcaa taattggtgc gcaagccata
     4021 agcagcgaac gggaagacgg gatattgcca ttgtttgtgc cttggttcct gggcgactcg
     4081 cgtgcaaatg gttatggcat tcattgggcg atgcgcggtt ccacggcttc acgaatcgtg
     4141 atgatctccc gctcccgctt gagccacaaa gcctgcgcct gcgcgagcga ggtcagttcg
     4201 aaccgcaggc ggtcgcctgg gcgcaattgc gcaagcaaat gcaggtccac cgtcgcgact
     4261 tcggcgattt tcggatagcc tccgacggtt tgccggtcgg ccatcagtac gattggcttg
     4321 ccagatggcg gaacctgaat ggtgccaaaa gtcaccgctt ccgacagaat ctcacctgga
     4381 cccccggtca ccagatgctc gctttccagg cggcagccca tgcggttgga gtcgggagca
     4441 acgcaaaatt cgccgctcgt cagcgcctcg cgcgctgtgc tcgggaatgc gtcccagtgg
     4501 cggcccgcca aaatgcgcac gatttgtggc gcgcgccgca gttgttcgat gtgctgattg
     4561 acagcccatt tggttgcgct aaagctgtgt ccggaatcga cgagttgttg ctccaacccc
     4621 ggataggtat tggcaccatg tgcggcacca agcggcaaca catccgcttt gcgcagcgcg
     4681 cgaccgtcca ggccgcccag ccgtgcggtg tcgtaagtgc tgcggctgcc catgaccaca
     4741 ggcacatcga agccgccgtg aaccgcaagg tagcagcggc acccggcaag cgcacggccg
     4801 aaatcgagta ccgtgccagc gcgcacgcgc acgggcttgc cttcgggcgc cgggatgccg
     4861 tcgagggtcg gcgacagctc cgcgccacac agtgctatca aagcatcttt ctcgaaacgc
     4921 agacggggac cgcgcagcgt aatctccaaa gtggcttccc gggtgtcatt cgcggccagg
     4981 atattggcaa tgcgatgtga aaagaaatcc attgctcccg ataccggcac ccccaagtgc
     5041 tggaaacccg tgcgtccgca atcctgcacg gtcgtcagca tgcccggctg cagtacggtc
     5101 agactcatgg tgccgctctc gattgcgctt caaattccac tgcggagatt tgcatgaagc
     5161 gcacctggtc cccggcgttc agcaggctcg gcggtgagct gtcgggtttg aaaagctgca
     5221 atggagtgcg gccgatgagc tgccatccgc ctggcgtatc gagcggataa acagcggtct
     5281 gctcaccggc gatggccaca ctgccagccg gtacatgtgc acgcggtgta tcccgccgcg
     5341 gcgtcgcgat cctgttatcc agcccgccga gataggcgaa ccctggtacg aagcccagca
     5401 tatgtacgta gtacgttgat ccgctatgaa tggcaatgac ttcgccgctc gacaagccaa
     5461 ccgagcgtgc caccgcatcc aggtccggac cgaacgcgtt gccatagcag accggaattt
     5521 cgatggtgcg cccggccgct tcgatagcag tggcttgcgt ctccagccag ctggcgatga
     5581 tgtcgcacag cgtctcgtac ggcgatgccc ctgatcccat ggctgcggga tcataatgca
     5641 acgccagcgt cgcgtaggcg ggcacgatat cgagaacacc gatcggccgg gaatcgcgca
     5701 gccgttgcgc aagtgcggcg atatgcgcgc tcagcgtgat gtcgatgcgc tcgccgaatt
     5761 ccacgaccag cgcctgatca cccatcggaa agaaacgcgg agcagcaagc atttgcggca
     5821 aaggtgcgtt aagcagtggg ttcagcgcgc gggggcgctc gaaagtcgtc ctgcatcgcc
     5881 ggccgcttcg atgcgcggcg cggtggcgcg cttcaacatt tcgtcgtaca gcgttagcgg
     5941 gttcatgtcg aatacgattt tggaatcgaa cgccagcacg taccagccgc cttgcgcaat
     6001 ctcgtcctcg agctgtccgg cagcccaccc cgagaacccc gtgaagaaac gctgctggct
     6061 ggcgttttc
//
LOCUS       DATMZQ010000209         4699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1888447, whole genome shotgun sequence.
ACCESSION   DATMZQ010000209 DATMZQ010000000
VERSION     DATMZQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888447"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VN496_06815"
     CDS             <1..1433
                     /locus_tag="VN496_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXF16697.1"
                     /translation="AAAYKAFGESEWTARLWTALAGFLGVFAVAFAGRRLFGRSAGFC
                     AALVLSSSLLYAVIGHVNTLDMGVTFCLTLGLLGFVLAQRGEQPTRESRLWMLLAWAA
                     MGFAFLSKGLIGFMMPAGAMVVYALIQRDLSFLKRLNAPAGIVLMLVIALPWIVAVSI
                     ANPEFARFFFIHEHFERFLTQVHHRTAPWWYFVPILLAGMLPWTTMLGQSLIAAWKRD
                     ASEGGFKARRFLLIYAAVTFLFFSVSQSKLPSYILPIFPALALLTGEWLSRVRGRTLA
                     WLIVPVILLALVCALASPYAVHLGSEKVPAALYLDFRNWLLAGALALLAASCLSLALA
                     WRERVEAAVVALCTGGLLLVQLAMTGHEALSPSFSTSHVAKIIRPLLTPDTPFYSVRT
                     YEHTLPFYIQRTVTLVDYGDELEFGLQQEPQLEIPTMSEFEARWRHNVKALAIMGPDV
                     YRELTGHALPMRLLYADARRVIVSRQ"
     gene            1430..1801
                     /locus_tag="VN496_06820"
     CDS             1430..1801
                     /locus_tag="VN496_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HXF16698.1"
                     /translation="MNLLSFSLILTGVLLNAAAQLLLKAGTNSIGAFAFTADNVVPIG
                     ARLAANPFIAGGLGCYVVSVVVWIMALSRVEVSLAYPMLSIGYVVNALAAWYLFGEAL
                     TAQRLIGIGTIIVGVFLLSRT"
     gene            1807..2955
                     /locus_tag="VN496_06825"
     CDS             1807..2955
                     /locus_tag="VN496_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HXF16699.1"
                     /translation="MSQAAAYLPFALPTIDEEAIAGVAEVLRSGWIASGPNVLKFERA
                     LSDFHDGRPARVVTSATAALELALQVAGVGPGDEVITPAMSFFAAPNMILKVGATPVF
                     VDCDLVTRNLDIDQVRAAITPRTRAIMPTHFAGLPVDIEGLYAIAQRHGVRVIEDAAL
                     AIGSRWRGRRIGAFGDLVVFSFHPNKNMTTIEGGAVLLNEESEAKLLEKLRFHGIVRL
                     ADGTRDVDVAAGKFNLPDVNARVGLSQLARLEEFNAMRRRLAARYFENLSGNRGCVLP
                     ARAHAGEEDGHSWNMFCILLPLDRLAITRKQFIDQMHARGIGIGVSYEALHLTTLCRK
                     LGYREGSFPNTERIGRETVTLPLFPTMKMSDVDRVCQALSEVLAANKC"
     gene            2980..3984
                     /locus_tag="VN496_06830"
     CDS             2980..3984
                     /locus_tag="VN496_06830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF16700.1"
                     /translation="MPEPILSVVIPVYNEEAGLTALFSRLYPALDGMRLPYEIIFIND
                     GSHDRSAAILRQQFEQRPDVTRVVLFNANYGQHLAIMAGFQHSRGQRIVTLDADLQNP
                     PEEIGKLLAKMDEGYDYVGGVRAERHDTWFRRHASRWMNRLRESTTRIHMTDQGCMLR
                     AYDRQIVDAINRCEEVNTFIPALAYTFSSRPAEVEVEHEERAVGTTKYSLYKLIRLNF
                     DLVTGFSLAPLQFFSLVGFVLAASSTGVVIFQIFHRLLRGEAAEGMFQSVAEAVQFFL
                     FGVVLFGIGLLGEYIGRIYQEVLRRPRFIVAAVLERRSSDDAPVPVQQASVPKTSALR
                     "
     gene            3992..>4699
                     /locus_tag="VN496_06835"
     CDS             3992..>4699
                     /locus_tag="VN496_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HXF16701.1"
                     /translation="MTTAVVFAYHDVGVRCLKVLLAQGVHVPLLVTHRDNAAEHIWFD
                     SVAAVARDYDIPTIMPEDPNLPATLQAVSSVKPDFLFSFYFRHMIGRPLLKTPTRGAL
                     NMHGSLLPKYRGRVPVNWAVIKGEKETGSTLHYMVEKADAGDIVDQQAVPILPDDTAL
                     EVFRKVTVAAEMVLHRSLPLLVAGNAPRRIQALARGSYFGGRKPEDGCIDWNETAESV
                     HNLVRGVAPPYPGAFTTV"
BASE COUNT          854 a         1358 c         1419 g         1068 t
ORIGIN      
        1 ccgctgcagc ctataaggct ttcggcgaaa gcgaatggac agcacggctg tggactgcac
       61 tggccggatt ccttggcgta ttcgccgtgg cctttgccgg caggcgcctg tttggcagat
      121 ccgcgggatt ctgtgccgca ctggtcctgt ccagtagcct gctctacgcg gtgatcgggc
      181 atgtcaatac cctggatatg ggcgtcacct tctgtctgac gctgggcttg cttggtttcg
      241 ttctggctca gcgcggcgaa cagccgacgc gcgaatcgcg cttgtggatg ctgcttgcgt
      301 gggcggcaat gggctttgct ttcttaagca aaggcctcat cggattcatg atgccagccg
      361 gtgccatggt ggtttacgcg ctgatacagc gtgatctttc cttcctgaag cggctcaatg
      421 cgcccgccgg gatcgtcctt atgctggtca ttgcgctgcc gtggatcgtc gcagtgtcga
      481 tagccaatcc ggagttcgcg cgcttttttt tcatccatga gcatttcgag cgcttcctga
      541 cgcaagtcca tcaccgcacg gcgccctggt ggtatttcgt tccaatcctg ctcgccggca
      601 tgctgccgtg gacaacgatg ctgggacaat cgctgatagc tgcatggaag cgcgatgcgt
      661 ccgaaggcgg attcaaggcc cggcgcttcc ttctgattta tgcggcggtc acgttcttgt
      721 tttttagcgt gtcccaatcc aagctgccgt cttatatcct cccgatcttt ccggcgctgg
      781 cattgctgac cggagaatgg ctgagccggg tccgcggccg gacactggcg tggcttatcg
      841 tgccggtcat cctgttggct ctcgtctgtg cgctggcctc gccttacgcg gtacatttag
      901 gcagcgagaa ggtgcctgca gccctgtatt tggattttcg caattggtta ctggctggtg
      961 cattggcgct gctggccgct agctgcctgt ctctggcact ggcctggcgt gagcgcgtcg
     1021 aagcagcagt cgtcgcactt tgcactggcg gcctgctgct ggtgcagttg gccatgaccg
     1081 gacacgaggc actgtcgccg tcattttcaa cgtcgcatgt ggccaaaata atacggccct
     1141 tgctgacacc ggacacgccg ttttacagcg tgcgcactta tgaacacacc ctgccgtttt
     1201 atattcagcg caccgttaca ttggtggact atggcgatga gctcgaattt ggcctgcaac
     1261 aggaaccgca actcgaaatt ccgacgatga gcgaattcga ggcacgctgg cgccataacg
     1321 tcaaggctct tgccatcatg ggtccggacg tctatcgcga attaaccggg cacgcattgc
     1381 cgatgcggtt gctttacgca gatgcccgcc gtgttatcgt atccagacaa tgaatttgct
     1441 gagtttctcg ctgatactga ccggcgtcct gctcaatgcc gcagcgcaat tgctgctgaa
     1501 ggccggcacc aacagcattg gcgcattcgc gttcaccgct gataatgtgg taccgatcgg
     1561 agccaggctc gctgccaatc cattcatcgc cggcggcctc gggtgctacg tcgttagcgt
     1621 cgtggtgtgg atcatggcgc tgtcacgggt cgaagtcagc cttgcgtatc caatgctttc
     1681 catcggctat gtggtcaacg cgctggccgc gtggtattta tttggtgagg cactgactgc
     1741 gcagcgcctg atcggtatcg gcacgatcat cgtaggcgtt ttcctgcttt cacgcacatg
     1801 acagtgttga gccaggccgc ggcttatctt ccgttcgcac tgccaaccat agacgaagaa
     1861 gccattgccg gcgtggccga agtgctgcgc tccggctgga ttgccagtgg acccaatgtg
     1921 ctcaaattcg agcgcgcgct ttcggatttt cacgacggcc ggccggcccg ggtcgtgacg
     1981 tccgccaccg ctgcactgga gttggcattg caggttgccg gcgttggtcc cggcgacgag
     2041 gtaatcactc cagccatgag tttcttcgca gcgcccaaca tgatcctcaa ggttggagcc
     2101 acgccggtgt tcgtggattg cgatctggtc acgcgtaatc ttgatatcga tcaagtgcgg
     2161 gccgccataa cgccgcgcac ccgagcgatc atgcccaccc attttgccgg cctgccggtc
     2221 gatatcgaag ggttgtatgc cattgcgcaa cggcatggcg tgagggtgat agaagatgct
     2281 gcactggcca tagggtcgcg ctggcgcggg cggcgcatcg gcgcttttgg tgacctcgtg
     2341 gtgttcagtt ttcacccgaa caagaacatg acgacgatcg aaggcggcgc cgtgttgctg
     2401 aatgaggaaa gcgaggcaaa gctgctggaa aagcttcgct ttcacgggat cgtgcgtttg
     2461 gcggacggta cccgggacgt ggatgtcgcg gcgggcaaat tcaacttgcc agacgtcaat
     2521 gcccgcgtag gtctgtcgca gttggcccgg ctggaagaat tcaatgctat gcgccgccgc
     2581 ttggcggcgc gctatttcga gaatctgtcc ggcaaccgtg ggtgcgtttt gccggctcgc
     2641 gcgcatgcag gcgaggaaga cgggcacagt tggaacatgt tctgcatatt gctgccgctg
     2701 gatcggcttg ccatcacgcg caagcagttc atcgatcaga tgcacgcacg cggcatcggt
     2761 atcggagtct cgtatgaagc actgcatctt acgacgttat gccgcaagct gggttatcgc
     2821 gaaggcagtt tcccgaacac cgaacgtatc ggcagggaaa ccgtaacctt gccgctattc
     2881 ccgaccatga agatgtccga tgtggatcgg gtgtgccagg cgctttccga agtcctggcc
     2941 gccaacaaat gctgatgatt gcattgccgg agcgaacaca tgcctgaacc gatcctgtcg
     3001 gtagtcattc cggtttataa cgaagaggct gggttgacgg ccttgttctc gcgactctat
     3061 ccggcgctgg acgggatgcg cctcccctac gaaatcattt ttatcaacga cggcagtcat
     3121 gaccgctctg cggcaattct gcgccagcaa ttcgagcagc ggcccgatgt gacgcgcgtt
     3181 gtgctgttca acgccaacta tggccagcac ctcgccatca tggcgggatt ccagcactcc
     3241 cggggccaac gcatcgtcac gctggatgcc gacttgcaga acccacccga ggagatcggc
     3301 aagctgctgg cgaagatgga tgaaggatac gattatgtag gcggtgtgcg tgccgaacgc
     3361 catgacacct ggtttcgccg ccatgcctcg cgctggatga accggttgcg cgaaagtact
     3421 acgcgaatcc atatgacaga ccagggttgc atgctgcgtg cctatgaccg gcagatcgtc
     3481 gacgcgatca atcgctgcga agaggtcaac acctttattc cagcgctggc ttatacattc
     3541 tccagccggc cggcggaagt tgaagtcgag cacgaggagc gcgcagtcgg cacgaccaag
     3601 tattcactgt acaaactcat acgtttgaat ttcgacctgg tgaccggctt ttcgctggcg
     3661 cctttgcagt ttttttcgct ggtgggcttt gtgctcgcgg caagctcgac cggtgtcgtg
     3721 atattccaga tcttccaccg gctgctgcgc ggtgaggccg ccgagggcat gttccaatcg
     3781 gtagcggaag cagtgcagtt tttcctgttc ggcgtggtgc tgttcggcat tggactgctg
     3841 ggggaatata tcggccggat ctaccaggaa gtgctgcggc ggccccgctt catcgttgcc
     3901 gctgtgctgg aacggcgctc atcggacgat gcgccggtgc ccgtccagca agccagtgtc
     3961 ccgaagactt cggccttgcg ctagagctga catgacgact gccgttgtgt ttgcttatca
     4021 cgatgtcggc gtacgctgcc tgaaagtcct gctcgctcag ggcgtgcacg tgccattgct
     4081 cgtgacccac cgtgataatg ccgcggagca catctggttc gacagcgtcg ccgcagtagc
     4141 acgcgattac gatatcccca cgatcatgcc ggaagatccg aatttgcccg caacgctgca
     4201 ggcagtcagc agcgtgaaac ccgattttct gttctcattt tattttcggc acatgattgg
     4261 tcgcccgctg cttaagacac ccacgcgcgg cgccctcaac atgcacggtt cgttgctacc
     4321 caaataccgc ggacgcgttc ccgtcaactg ggcggtcatc aagggcgaaa aggaaaccgg
     4381 ttcgacactg cattacatgg tcgagaaagc ggacgcaggc gatatcgtgg accagcaggc
     4441 ggtgccgatc ctgcccgacg acaccgcgct cgaggtgttc cgcaaggtca ctgtcgcggc
     4501 ggaaatggta ttgcatcgca gcctgccact gcttgtcgcc ggaaacgcac cgcgccggat
     4561 ccaggcgttg gcgcgagggt cgtattttgg cggtcgcaaa cccgaggacg ggtgcatcga
     4621 ctggaatgag accgcagaaa gcgtccataa tctggtgcgt ggggtggcac cgccctatcc
     4681 gggcgccttt accaccgtg
//
LOCUS       DATMZQ010000210         4553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3267264, whole genome shotgun sequence.
ACCESSION   DATMZQ010000210 DATMZQ010000000
VERSION     DATMZQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267264"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VN496_06840"
     CDS             <1..571
                     /locus_tag="VN496_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16702.1"
                     /translation="GFAAGVMVWHLYKNADKSRNWLCSTAVANIVEWVALALVILFVS
                     LANNTTVLSIAAPLVFALVVLVFAFEKGAVSGMLQARPLLFLGTVSYSIYMTHVFVAQ
                     RLFDAVNAFGRFSRINPFMHRDIAGYDAYFLGTRLWHGDVAYVGYIALVIVTSSLTYR
                     WIEKPAREWVRMRVRRGWPPLRPRELPSV"
     gene            complement(823..1536)
                     /locus_tag="VN496_06845"
     CDS             complement(823..1536)
                     /locus_tag="VN496_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXF16703.1"
                     /translation="MRKLERRFEGHFPIEKDLSYLDLGCGSGDLSMALAERGVAQVTG
                     IDFIPRNIERAKARAAMSGVAERVSFVCRDLREWVPEKKFDVLLSIDVLEHIDNPGAF
                     LKKIADFVSYEGVAVLAFGPLFHSPFGDHMWDFFRVQIPWRGLLFSESLILRLRRECF
                     RPTDPAMRYRDIVGGLNLMRYSEFLQHVAAAGWHFDYLAMNTFLPKIVRPLSDALTRY
                     PFLGNYFAHNVYAILRRNR"
     gene            2364..3914
                     /locus_tag="VN496_06850"
     CDS             2364..3914
                     /locus_tag="VN496_06850"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXF16704.1"
                     /translation="MDSSLQSATRPDTSAAAQPTDSEAKTVAMPSLQRLGHRALSLGV
                     ANAFDFATQFLLPVVLTRCLEPNAFGEYRLLWLTAGTIVALATLCMPPSLYYYLPRSD
                     VHQKRMYVNQTMFFLLCAGLLSGWAVSAWNPWLPDQLRELARHGAIVPTFIVLLIVAS
                     LLDVLPTVEERVYWQAKAIVSLAVLRAVALSLAAALTGELAPVLMVLVAFVAFKVFVL
                     LFYVVRYHGLRGPVLRWREFVDQLLYAAPFGAAGALYGLRTQSDQWVAASLFPLGLFA
                     AFSIAAVLGPLMNLFRQSVNYAFLPIMSRRHAEGDIPGMLELNSRGNVMVGAIVFPLF
                     YFAFVFAEDLITLVYTANYVAAVPVVRIYILGIAALVLELSSLTMLLRQAVFVMVLNA
                     LALVLAVALNWYAATRVGLAGAAMGTVIVMGIDRIVTLWRISRLTGVPIRRLQDWKTL
                     LLLVLFAAGASLLAWAVTVLYFATSGPVLRMLAGGAVLALSYVAIAALSGTGRTWLAA
                     VRTQKHGI"
     gene            3922..>4553
                     /locus_tag="VN496_06855"
     CDS             3922..>4553
                     /locus_tag="VN496_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXF16705.1"
                     /translation="MARLMFVTASLAFGGAERHSITLMNRLAERGHECHAVYVKSAAE
                     QLDCIRLRGAGGIRCLNARRYVDRGAIADFAAHIARIQPAVIVAANPYALMYSWLALR
                     RSGMRAGIAVTFHSTLLTSAKEWLQMLYYRPFFWVADCLVFICEAQRRYWTRRLLTAR
                     RTEMIYNGVDLAHWQPLSERDRRLKRLDLGFTMDDFVIGMCAVFRPEKNHL"
BASE COUNT          890 a         1278 c         1322 g         1063 t
ORIGIN      
        1 tgggtttgct gccggcgtaa tggtatggca cctatataag aacgcggaca agtccaggaa
       61 ttggctctgc tcaaccgccg tcgcgaacat tgtggaatgg gtcgcgcttg cgcttgtcat
      121 cctttttgtc agcctggcaa ataacacgac ggtactgtct atcgctgcgc cgttggtatt
      181 tgcactcgtg gtgctggtct ttgcgtttga aaagggcgcg gtgagcggca tgctgcaagc
      241 gcggccactg ctctttcttg gcaccgtgtc gtattccatt tacatgacgc atgttttcgt
      301 ggcgcagaga ttattcgacg cagtcaatgc ttttggcaga ttctcgcgca tcaatccatt
      361 tatgcatcgc gatatagcag ggtacgatgc ctatttcctt gggacgcggt tgtggcacgg
      421 tgacgtcgct tacgtgggct acattgcgct ggtcattgtc acttcatcgt tgacttatcg
      481 ttggatagag aagcccgctc gcgagtgggt caggatgcgc gtccgtcgag gctggccgcc
      541 attgcggccg cgcgagctac ccagcgtgtg acagtcaatt caggacctca ggatgggcag
      601 gcagggcgtc tgtgccatat ttaaatcaac cactcagcta gtttgctacc cgtagtgcca
      661 agcgcagacc ggttcacccg ttcagccgtg cagctcaccc aattcccggg ctgaacgcaa
      721 aaatcctcat ggcaaaacaa catgagccaa cgcaatacac tggaccgctt gccaggtcat
      781 tgcaggttac gggtatgtcg ccgacgccgc acggctaacg gctcaacgat tgcgccgcag
      841 aatggcgtaa acgttgtgag cgaagtagtt tcccaaaaac ggataacgag tgagcgcatc
      901 tgaaagcggt cgaactatct ttggcaggaa tgtattcatg gcgaggtagt caaaatgcca
      961 gcccgccgcc gcgacatgtt gcagaaattc ggagtagcgc attagattga ggccaccgac
     1021 aatgtcccgg taccgcatgg ccggatcagt tgggcggaag cactcacgcc gaagccgaag
     1081 aatcaatgat tcagaaaaga gcaatcccct ccacgggatt tggacgcgga agaaatccca
     1141 catgtggtct ccaaacggag aatggaacag cggaccgaac gcaagcacgg cgactccctc
     1201 atagctcacg aagtccgcta tttttttgag aaaagcgccg ggattatcaa tatgctccaa
     1261 gacatcaatg gacagcaaga cgtcaaattt cttctctggc acccattctc gaaggtccct
     1321 gcagacgaag ctaacccgtt ctgctacgcc actcatcgcc gcacgggcct ttgcgcgttc
     1381 gatgttgcgc ggtatgaaat cgatgccggt cacctgtgca acgccgcgct cggccagggc
     1441 catggacaga tcgccgcttc cgcagcccag atcaaggtac gaaagatcct tctcgatcgg
     1501 aaaatgaccc tcgaagcggc gttccagttt gcgcaacttc gcacggccat catcaggcgt
     1561 gccagcatca cttccgaatc gcgcatttag ttcctcctct gttgcgcggt ttcgcgcatg
     1621 ggttaagagg gaaaggaaca agtaaggtac cgcctgaatt cctaaggcgg gatgtgactt
     1681 ggcgatctca gaagatctcg aggaacgccg atttttcacg ccatgaacat caggagctcc
     1741 ttttggggtg gtgtcgggac tgtcctcatc gctgacgtgg cgctttgtgg ccaaacgggc
     1801 acgcatcaac agctcctgcg ctgaatgaag cgaaaaacaa tgcgacttct tcttgaaaca
     1861 gagtcgggcg gtcatcgtcg gccacatggt ccaaagggcc catgagaagt gtccgggcaa
     1921 cggcacgatt gacagcgcag ccctctattt ctcgggagtt cgccacgctg cactttgggt
     1981 ccgcaattgc gtgttcgata tcagcgtagc cgcagtctga accacaccgc gttgtgaccg
     2041 tccgccactg gtgcagtcaa cggccgtgat tgacgcgact cctggctgtt ggctccaagt
     2101 agtccaattg gcctagatgc gcagaaggcg ggaaggaacg tcgcaaggct agcatgacat
     2161 cccataccgc tgtagtgcgt cacgccattt ttcttggccc tgcaggttga tcacagccga
     2221 agcgggacga ccgagcatcc ttgcgaaccg gaattaaaaa aagcggaacc cttcaaatca
     2281 cagccgctct acattgagtg ttcccgaagc gcaatgatga cgatgtatcg gtacccagat
     2341 aaatggcgca agagttgact ctcgtggatt cgagtctgca aagcgcaacc cgtccggata
     2401 cgtccgccgc cgcgcagccg acggacagcg aagcgaaaac ggttgcgatg ccttcattgc
     2461 agcgactagg acatcgcgca ttgtctctgg gtgtggcgaa tgcgttcgac ttcgcgaccc
     2521 agtttctact cccggtggtg ctgacacgat gcctggagcc caatgcattc ggagaatacc
     2581 gcctgttgtg gctcacggcc ggcaccatcg tggcacttgc gaccctttgc atgcccccaa
     2641 gcctgtacta ctacctgccg cgatcggacg ttcaccagaa gcgtatgtat gtgaaccaga
     2701 cgatgttttt tttgctgtgc gcagggctgc tgtcaggatg ggccgtcagt gcctggaacc
     2761 cctggttacc ggaccaattg cgcgagcttg cgcggcacgg cgcaattgtg cccacgttca
     2821 tcgtgctgct gattgttgcc tcgctgcttg atgtgctgcc cacggtggaa gagcgcgtgt
     2881 attggcaggc caaagcgatc gttagccttg ctgtactgcg cgccgtagca ctgtccctcg
     2941 cggcggcgtt gaccggagaa ttggcaccgg tactgatggt gctcgttgcc ttcgttgcct
     3001 tcaaagtgtt cgtgctgctt ttctatgtcg tccggtatca cggtctgcga ggaccagtgt
     3061 tgcgctggcg tgagtttgtc gaccagctgt tgtatgccgc cccgtttggc gctgccggcg
     3121 cattgtacgg actgcgcacg cagtcggatc agtgggtggc ggcgtcactg ttccccctcg
     3181 gcctgtttgc cgccttctcg atcgccgcag tgctggggcc tttgatgaac ctgttccggc
     3241 aatcggtcaa ctacgctttt ctgccgatca tgagccgtcg ccacgcagaa ggcgacattc
     3301 cgggcatgct ggaactcaac agccgcggca acgtcatggt gggagcgatc gtatttccgc
     3361 tgttctactt tgcgtttgta tttgcggaag acctcattac cctggtgtac accgcgaact
     3421 atgtggcggc ggttccagtt gtccgaatct atatcctcgg cattgcggcc ctggtccttg
     3481 aactttcgag ccttaccatg ctgctgcggc aggcggtgtt cgtgatggtg ctgaacgcgc
     3541 tggccctggt attggcagtg gcgctgaatt ggtacgcggc aacgcgggtc gggcttgctg
     3601 gtgcggcgat gggcaccgtc atcgttatgg gcatcgaccg cattgtcacg ctgtggcgta
     3661 tctcgcgact caccggtgtg ccgatcaggc gactccagga ctggaaaaca ctgttgctgc
     3721 tcgtactgtt tgcggcgggg gcttcgctgc ttgcatgggc tgtgactgtt ctttatttcg
     3781 ccacttccgg gccggtgctt cgcatgctgg ccggcggcgc cgtacttgct ctttcttacg
     3841 ttgccattgc tgcgttgtcc ggtacgggcc gcacgtggct tgccgcagtg cgaacccaaa
     3901 agcacggcat ctgatcccga catggcgcga ctgatgttcg tcaccgcatc attggctttt
     3961 ggcggtgcgg aacgccactc catcacgctt atgaaccggt tggccgagcg cggccatgag
     4021 tgccatgctg tttatgtaaa aagcgcagcc gagcagcttg actgcattcg cttgcgcggc
     4081 gcaggtggca ttcgttgtct aaatgcgcgg cgctatgtcg accgtggggc catcgctgat
     4141 tttgcggcgc atattgcacg cattcagccg gccgttattg ttgccgcgaa tccgtatgcg
     4201 ctgatgtact cctggctcgc attgcgtcgt tccgggatgc gcgccgggat tgcagtgaca
     4261 ttccacagta ctttgctgac gagtgcaaag gaatggttac agatgctcta ctatcgcccg
     4321 ttcttctggg tggcggattg ccttgttttc atctgcgagg cgcagcggcg ctactggacg
     4381 cgccgtcttc ttactgcccg gcgcactgaa atgatctaca acggagttga tcttgcccac
     4441 tggcagccgc tcagcgagcg cgatcgccgg ctcaaaaggc tcgatctggg cttcaccatg
     4501 gatgatttcg taatcggcat gtgcgcggtt ttcagaccgg aaaaaaatca tct
//
LOCUS       DATMZQ010000211         3610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4915747, whole genome shotgun sequence.
ACCESSION   DATMZQ010000211 DATMZQ010000000
VERSION     DATMZQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4915747"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VN496_06860"
     CDS             complement(<1..392)
                     /locus_tag="VN496_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16706.1"
                     /translation="MAVALVARKAAKKALKPSPASTKVAGNVPQIKPGTYADGIPFSE
                     LQYLACKLILRPNHFTSRKSLFDFADVIQGPAAKHGVQFSTAPYLKAPVQIREVLFLD
                     TPDFRFYKNAFILRRRIAYKDGFPIGEP"
     gene            666..2093
                     /gene="ccoN"
                     /locus_tag="VN496_06865"
     CDS             666..2093
                     /gene="ccoN"
                     /locus_tag="VN496_06865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HXF16707.1"
                     /translation="MQEQTTYNYKVVRQFAIMTIVWGIVGMAVGVLIAAELMFPDLTQ
                     GISWLSYGRLRPLHTNAVIFAFGGCALFATSYYVVQRTCHVRLFSDRLAAFTFWGWQA
                     VIVLAAITLPLGITTSKEYAELEWPIDVLITVVWVSYAAVFFGTLAKRRISHIYVANW
                     FFGAFILTVALLHVVNSAEIPVTFWKSYSAYAGVQDAMVQWWYGHNAVGFFLTAGFLG
                     IMYYFVPKQADRPIYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVFSLI
                     LLAPSWGGMINGIMTLSGAWHKLRTDPILKFLVVSLSFYGMSTFEGPMMSIKTVNSLS
                     HYTDWTIGHVHSGALGWVAFISIGSLYYMIPKLFGRAEMFSVRLITAHFWIATIGVVL
                     YIAAMWIAGVMQGLMWRAVNVDGTLTYTFVESVKATYPFYAIRLLGGILFLAGMLLMA
                     YNTWKTVVGSKPFNALIPSPAIAHA"
     gene            2104..2724
                     /gene="ccoO"
                     /locus_tag="VN496_06870"
     CDS             2104..2724
                     /gene="ccoO"
                     /locus_tag="VN496_06870"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HXF16708.1"
                     /translation="MAQIALQEKIEKNVGLLIVLCLLVVAVGGLVEIVPLFFQRSTTE
                     PVAGLKPYSAVALEGRDIYIREGCYNCHSQMIRPLRAETERYGHYSVAGEFVYDHPFQ
                     WGSKRTGPDLARVGNRYSDEWHRLHLMQPRDVVPESNMPSYSWLATAKIDESVTPSKM
                     KALLRVGVPYTDEDIAGASEAVKGKTEMEALVAYLQGLGTAIKTVR"
     gene            2728..2895
                     /locus_tag="VN496_06875"
     CDS             2728..2895
                     /locus_tag="VN496_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HXF16709.1"
                     /translation="MDLNDFRIVTTVLTFLTFIGIVAWAFSNRRKKDYEAAARMALDD
                     DQPIQPDGSHT"
     gene            2892..>3610
                     /gene="ccoP"
                     /locus_tag="VN496_06880"
     CDS             2892..>3610
                     /gene="ccoP"
                     /locus_tag="VN496_06880"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HXF16710.1"
                     /translation="MSDFNSEFWSYYVSIITLVSIAACAILLWTMTTRRVAQKVETMG
                     HVWDENLAEFNNPLPNWWRWMFYITIVFGVLYLVFYPGLGRFAGTFGWTSQGQYDDEQ
                     TAAAKNYGPLFAKYAAMDIPSVAADPQAREIGQRLFLNYCSQCHASDARGGRGFPNLT
                     DGDWLYGGDPATIQATIMSGRNGIMPPLGAALGEDGVKNVANYVLLLSGSEHDAALAA
                     KGKETFTTICAACHGVDGKGN"
BASE COUNT          715 a         1014 c         1057 g          824 t
ORIGIN      
        1 tcgggctcgc cgatgggaaa tccgtccttg taggcaatgc gcctgcgcag gatgaacgcg
       61 ttcttgtaga agcgaaagtc cggagtatcc agaaagagca cttcccggat ctggactggt
      121 gccttcaaat acggcgccgt cgagaactgc acgccgtgtt tcgcggcggg cccttgtatc
      181 acatcggcaa aatcaaaaag gctcttccgc gaggtgaaat ggtttggcct caggatgagc
      241 ttgcaggcga ggtactggag ttcgctgaaa gggataccgt ccgcatacgt tcccggtttg
      301 atttgaggca cattacccgc gaccttggtg gaagcgggtg acggtttgag cgctttcttt
      361 gcggccttcc ttgctacaag tgcgactgcc atgtaatcct ccccattttt tgagacccgg
      421 ctcataaatc cctggcacgc gttctccggg ctacgacatg taccgtattt gagggcaaac
      481 caacagcaaa acggtctttg gctacccgta tttacagctg gcacagttgc gctgttggcg
      541 catgttgcac aatcctgcgc cgatttgcag caaattagtt gatctaggtc aatcgatcct
      601 tgcgcccccc gggctaccat cccgcccggc gctgctggat accaaaaatc atcgagggag
      661 caaacatgca ggagcaaacg acatacaact acaaggtcgt acgccagttc gccatcatga
      721 ctattgtatg gggaatcgtc ggcatggcgg tcggagtcct gattgccgcg gaactcatgt
      781 ttcccgattt gacccagggg atttcgtggc tgtcatacgg gcgtttgcgt ccgctgcaca
      841 ccaatgccgt gatatttgcc tttggcggtt gcgcgctgtt tgccacttct tactatgtcg
      901 tccagcgcac ctgccacgtg cgcctgttca gcgatcgcct tgcggcgttc acgttctggg
      961 gctggcaggc cgtcatcgtg ctggccgcga ttaccctgcc gctgggcatt accaccagca
     1021 aggaatatgc agagctcgag tggccgatcg atgtgctgat cacggttgtc tgggtttcgt
     1081 acgcagcggt atttttcggc acgctggcca agcgccgaat ctcgcacatt tacgtcgcca
     1141 actggttctt cggcgcgttc atcctgacgg tcgccttgct gcacgtcgtc aacagcgcgg
     1201 agattccggt cacattctgg aaatcctatt ccgcctatgc cggcgtgcag gatgcgatgg
     1261 tgcagtggtg gtacggccac aatgcggtag gcttttttct aactgccggg tttctcggca
     1321 tcatgtacta cttcgtgccc aagcaggcag accggccaat ctattcttat cgtctgtcgg
     1381 ttgtgcattt ctgggcgctg atcttcacct atatgtgggc cggcccgcat cacctgcatt
     1441 acacggcatt gcccgactgg acgcagtcac tcggtatggt gttctcgctg atcctgcttg
     1501 cgccgtcctg gggcggcatg atcaacggca tcatgacgtt gtccggtgcc tggcataaat
     1561 tgcgtaccga cccaattctt aaattcctcg tcgtgtcttt gtcgttctat ggcatgtcga
     1621 cgttcgaggg accgatgatg tccatcaaga ccgtaaattc gctgtcgcac tatacggact
     1681 ggacgatcgg ccacgtgcat tccggtgcac tcggctgggt cgcattcatt tccatcggca
     1741 gcctgtacta catgattccg aaattgttcg ggcgcgccga aatgttcagc gtacgcctga
     1801 tcactgccca tttctggatc gcgaccattg gcgtggtgct ctatatcgcc gcgatgtgga
     1861 tcgccggggt catgcagggt ttgatgtggc gcgcggtgaa tgtggacggc accctgacgt
     1921 ataccttcgt cgagagcgta aaggcgacgt acccgtttta cgcgatccgg ctgctgggcg
     1981 gaatcctgtt cctggccggg atgctgttga tggcttacaa cacgtggaaa accgtggtcg
     2041 gcagcaaacc gttcaacgcg ctgattccgt cgcccgcgat tgcgcacgcg taaaggaaac
     2101 gacatggccc aaatcgcctt gcaagaaaaa attgaaaaga acgtcggcct gctgattgtg
     2161 ctgtgcctgc tcgtcgttgc cgtcggcgga ctggtggaga ttgtgccgct gtttttccag
     2221 cgttcgacga cggaaccggt tgccggcctc aagccttaca gtgcggtcgc gctcgagggc
     2281 cgggacatct acatccgcga aggttgctac aactgccatt cacagatgat ccggccattg
     2341 cgcgcggaaa ccgaacgcta cggtcattac tcggtggccg gagagtttgt ctatgaccat
     2401 cctttccagt ggggcagcaa acgcacggga ccggatcttg cgcgcgtggg caaccgctac
     2461 agcgatgaat ggcaccggct gcacctgatg cagccccgtg atgtcgtgcc ggaatccaat
     2521 atgccgtcgt attcatggct cgccactgcg aagatcgatg aatccgtgac gccttcgaaa
     2581 atgaaagcat tgctccgggt cggtgtgcct tacaccgacg aagacatcgc cggggcttcg
     2641 gaggctgtca agggaaagac cgaaatggaa gcactggtgg cctatctgca aggcctgggc
     2701 actgcgatca agacggtgag gtgagacatg gacctgaatg atttccgaat tgtgacgacg
     2761 gtactgacat ttctgacgtt catcggcatc gtcgcgtggg ctttcagcaa ccggcgcaag
     2821 aaggactatg aagcggcagc gcgcatggcc ctcgatgatg accagccaat ccagccggac
     2881 gggagccaca cgtgagcgac ttcaacagcg aattctggag ctattacgtt tcgatcatca
     2941 cactggtcag catcgcggcc tgcgccatcc tgctatggac gatgaccacg cggcgcgtgg
     3001 cacaaaaagt ggagaccatg gggcatgtgt gggacgagaa cctggccgag ttcaacaacc
     3061 cgctgccgaa ctggtggcgc tggatgttct acatcaccat tgtgttcggc gtgctgtatc
     3121 tggtgttcta tccgggcctt ggccgtttcg cgggaacctt tggatggacc tcgcaaggcc
     3181 aatacgacga tgagcagacg gccgcggcga agaactacgg gccactgttt gcaaaatacg
     3241 cggccatgga cattccatca gttgctgctg atcctcaggc ccgtgaaatc gggcagcgct
     3301 tgtttttgaa ttactgttcc caatgccatg cctccgacgc gcgcggcgga cgcggctttc
     3361 cgaacctgac cgatggcgat tggctgtatg gcggcgaccc ggccaccatt caggccacca
     3421 tcatgtccgg gcgcaacggc atcatgccgc cgctcggtgc ggcgcttggc gaagatggcg
     3481 tcaagaatgt cgccaactat gtgttattgc tatccgggtc ggaacacgat gctgccctgg
     3541 cggcgaaagg caaggaaacc tttacgacga tttgcgcagc gtgtcacggc gtggacggca
     3601 aaggcaacca
//
LOCUS       DATMZQ010000212         5631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1197432, whole genome shotgun sequence.
ACCESSION   DATMZQ010000212 DATMZQ010000000
VERSION     DATMZQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197432"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..588)
                     /locus_tag="VN496_06885"
     CDS             complement(142..588)
                     /locus_tag="VN496_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXF16711.1"
                     /translation="MALKLQITEDMKSAMRAKDVQRLLAVRLLLAAIKQKEVDERIEL
                     SDSDVLAIVEKMIKQRRDSIEQFEKGGRQDLVDKEQSEIEVLRGYMPAALSSAEVDAA
                     VSDAIATSGAKLMADMGKVMAVLKPKLAGRADMAKVSALVKTKLTG"
     gene            complement(667..879)
                     /gene="rpsU"
                     /locus_tag="VN496_06890"
     CDS             complement(667..879)
                     /gene="rpsU"
                     /locus_tag="VN496_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXF16712.1"
                     /translation="MPTIRVKENEPFEVAMRRFKRTVEKTGLLTELRAREFYEKPTSE
                     RKRKLAAAVKRHHKRLRSQLLPPKLY"
     gene            1024..2046
                     /gene="tsaD"
                     /locus_tag="VN496_06895"
     CDS             1024..2046
                     /gene="tsaD"
                     /locus_tag="VN496_06895"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXF16713.1"
                     /translation="MLVLGVETSCDETGVALYDTDAGLLGHTLFSQIALHAEYGGVVP
                     ELASRDHVRRLLPLTRQLLSATGRKLADIDGIAYTMGPGLAGALLVGASVSASLGFAL
                     GVPVVGLHHLEGHLLSPLLADRAPSFPFVALLVSGGHTQLMKVNAVGQYELMGESVDD
                     AAGEAFDKTAKLLGLPYPGGPALAQLAESGNPNGIKLPRPMIASGDLNFSFSGLKTAA
                     QMLLNRAQDSTQSKADIAAAFQGAIVDVLVAKSLYALSKTGLDRLVVAGGVGANHCLR
                     QTLTQQAAMRGYEVFYPPLELCTDNGAMIAFAGAQRLVSGIMGPLGFSVRPRWELEEL
                     NRPETM"
     gene            complement(1973..2632)
                     /gene="plsY"
                     /locus_tag="VN496_06900"
     CDS             complement(1973..2632)
                     /gene="plsY"
                     /locus_tag="VN496_06900"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXF16714.1"
                     /translation="MTQILFIAVAYLIGSVSFAVVVSWCYGLPDPHTYGSRNPGATNV
                     LRTGRKEAAVLTLLGDAAKGAIALSLAKRLALEFNLDEFTLAAVALATFIGHLYPLYF
                     RFKGGKGVSTAAGILLALDLRVAGVALIAWLLTIVALRYSSLASIVAALAATMATLYF
                     LGWNPFAVMVLIMTTLLLWRHRTNMKRLLDGTESRITLFRGGSTPPIPSGDEPKSREV
                     P"
     gene            complement(2648..3544)
                     /gene="xerD"
                     /locus_tag="VN496_06905"
     CDS             complement(2648..3544)
                     /gene="xerD"
                     /locus_tag="VN496_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXF16715.1"
                     /translation="MISDEALLDGFSDALWLEDGLARNTLESYRRDLTLFATWIAATR
                     GKSLLQVQRMDLLDYLAHRFKHKSRARSAARLLSSLKRFYRYQQREKRIDADPTLQVD
                     SPKLPRSLPKMLTEEDVDTLLAAPNIDMALGLRDRAMLETLYASGLRVSELVSLSLGQ
                     LSRDAGVVRVIGKGGKERLVPVGEEALSWIERYLHAARPDILVGRQADALFVTSRAAP
                     MTRQAFWHLIRRYANQAGIGKGISPHTLRHAFATHLLNHGADLRVVQMLLGHADISTT
                     QIYTHVARERLKQLHAKHHPRG"
     gene            3747..4454
                     /locus_tag="VN496_06910"
     CDS             3747..4454
                     /locus_tag="VN496_06910"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HXF16716.1"
                     /translation="MTELDQSTISICSFEATQLPPGERLQMEIGSDGNQYYTTLIGFV
                     PGHSVLVRTPLMQNLPIPVPEGARVLVRAFSGRHAYTMESQVDRVCRSPYPYIHIAYP
                     AQVQQTLIRGALRVRVALPGVASNAIESDISITRAVVLSDLSTSGAQLESEQSLGDPG
                     EKFRLAFKFVVQPNNYEVKLATSAQIQSVRKTKRKNSQEEIFSNGVRFNKLHATEGLL
                     LQSYIQQVLLADRNRVI"
     gene            complement(4513..4890)
                     /gene="rplS"
                     /locus_tag="VN496_06915"
     CDS             complement(4513..4890)
                     /gene="rplS"
                     /locus_tag="VN496_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXF16717.1"
                     /translation="MNLIQKIEQEEIKRLGKDIPLFAPGDTVIVNVNVVEGDRKRVQA
                     YEGVVIAIRSKGLNSSFIVRKMSSGEGVERTFQTYSPLIASIELKRRGDVRRAKLYYL
                     RDRSGKSARIREKLESEKATDQE"
     gene            complement(4887..>5631)
                     /gene="trmD"
                     /locus_tag="VN496_06920"
     CDS             complement(4887..>5631)
                     /gene="trmD"
                     /locus_tag="VN496_06920"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXF16718.1"
                     /translation="ALTGSGITQRARSSGLYELVLWNPRDFSTNAYRSVDDRPYGGGP
                     GMVMMVEPLAKALRAARQRQSSSGVKHGRTVFLSPQGRPLTQQIVEELGKHDGLVLLA
                     GRYEGVDERVLQTEIDDQISIGDYVLSGGELPAMVLMDALIRRIPGALNDAESVQQDS
                     FSNGLLDCPQYTRPEVYGRDAVPAVLLSGHHGHISRWRMKQSLGRTWRVRPDLLVNLS
                     LSPEQMALLEEYKMEEYKPESKKAQEHIK"
BASE COUNT         1254 a         1599 c         1461 g         1317 t
ORIGIN      
        1 tgggaagtct cgccgtcctt tcctgcggtt gaaggaagta gccgtcgcag gacgttcgga
       61 attataggtc agttttcccg ggccccggga agccggcttc accaaccgct gaattttgcg
      121 gaggcgagtt cagtacctgt actagccggt gagtttggtc tttactaacg cggacacctt
      181 ggccatgtcc gcccgacctg ccagttttgg cttgagcaca gccattacct tgcccatatc
      241 ggccatcagt ttcgcgccgc tggtcgcgat ggcatcgctc actgccgcgt cgacttcggc
      301 agacgacagg gcagccggca tatagcctcg aaggacctca atctcagact gctctttatc
      361 caccaagtcc tgccgaccgc ctttttcaaa ctgctcaata gaatctcgcc gctgcttgat
      421 cattttctcg actatcgcta aaacgtccga gtcggaaagt tcgatgcgtt catccacttc
      481 tttctgtttg atggctgcga gcaggaggcg aacagccaaa agccgctgca cgtccttggc
      541 gcgcattgcg ctcttcatat cttcggtaat ttgtaatttg agcgccatga aggagattgg
      601 gatgattaaa tgcaaaacgg tgaatggctt cccattcacc gtctgaatct tatctttctg
      661 ccgctcctaa tacagcttgg gaggtagcag ctgactgcgc agacgtttgt gatgccgttt
      721 taccgctgcg gccagtttcc gtttgcgctc ggaggtcggt ttttcgtaaa attcacgcgc
      781 ccgtaactcg gtcagcagac cggttttttc caccgtccgt ttgaaacgcc tcatggccac
      841 ttcgaacggc tcattttcct tgactcgaat cgtcggcata cagacttcct tagcggtcag
      901 ccaaaaaacg aagggtgcga ttctagcaat aatcccctgc ttttggaaac attcttgagg
      961 catccgctgc caatcacgac gcgccggata ttatccaatc cccgcaatac gactcaatga
     1021 cttatgcttg ttctaggcgt ggagacttcg tgtgatgaga ccggcgtagc cttatacgac
     1081 acggacgccg gcctgctggg gcataccctt ttctcgcaga tcgccctgca cgccgagtac
     1141 ggcggggtgg tccctgaatt ggcttcgcgc gatcacgttc gacgattgtt gccgctaacg
     1201 cggcaactgc tgtcagcaac cggacgcaag ctggctgaca tagatggcat cgcgtacacc
     1261 atgggtcctg gactggccgg cgcgctgctt gtcggagcaa gtgtatcggc aagcctgggg
     1321 tttgcattgg gcgtcccggt cgttggcctc catcatctgg aaggacatct gctctcgccg
     1381 ctgctcgcgg accgtgcacc gagttttcct ttcgtggcct tactggtttc gggtggtcat
     1441 acgcaactca tgaaagttaa cgcggttggc caatacgaat tgatgggaga atcggtagat
     1501 gatgcggcag gcgaagcgtt tgacaagaca gccaagctgc tgggattgcc atacccgggc
     1561 ggacctgcgc tcgctcaatt ggccgagtct ggaaatccaa atggaatcaa actaccgcgc
     1621 ccaatgattg caagcggcga ccttaacttc agcttttcag gcttaaagac cgccgcacag
     1681 atgcttttga atcgagcgca ggacagtacg caaagcaagg ccgacatcgc cgcggcgttt
     1741 cagggtgcga tcgttgacgt tctggtagca aagtcactct atgcgttgtc caaaactgga
     1801 ctagaccggt tggtcgttgc gggaggagtc ggtgccaacc attgcttgcg gcaaaccctg
     1861 acacaacaag ccgcaatgcg gggatatgag gttttctatc cgcccttgga actatgcacc
     1921 gataacggtg ccatgatcgc attcgcaggc gcgcaacggc ttgtgtccgg catcatggga
     1981 cctctcggct tttcggttcg tccccgctgg gaattggagg agttgaaccg ccccgaaaca
     2041 atgtgatccg gctttccgtg ccatcgagca ggcgtttcat attggttcgg tgacgccaaa
     2101 gcagcagcgt tgtcatgatc agaaccatta ccgcaaacgg attccatccc agaaagtata
     2161 gtgtggccat tgtcgcggcg agcgcagcca caatcgatgc cagagacgaa taacgaagcg
     2221 caacgatcgt taaaagccat gcaatcagcg cgactccggc gacgcgcaga tccagagcga
     2281 gcaaaatgcc tgcggcagtg gatacgcctt tgccgccttt gaagcgaaaa tataacggat
     2341 acagatggcc gatgaaggtg gccagtgcca ctgcggccag ggtaaattcg tccaggttga
     2401 attccagcgc aagccgcttc gccagggaca gcgcgattgc gccctttgcg gcgtcgccca
     2461 gcagagtgag caccgcagcc tcttttctcc ctgtcctaag cacattggtt gcgcctggat
     2521 tacgggaccc gtaagtgtgc ggatcgggca acccgtaaca ccagcttact acgacagcga
     2581 acgacaccga accgataaga taagcaaccg ctatgaacag gatttgggtc ataaagatat
     2641 tccgggttta cccccgtgga tgatgtttgg catgcaactg cttaagtctt tcgcgtgcga
     2701 catgcgtgta gatttgtgtt gttgagatat ctgcgtgacc caacaacatc tgcaccacgc
     2761 gcaggtcagc cccatggttt agcagatgcg tagcgaaagc atggcgcagc gtatgcggtg
     2821 atatcccttt tccaatacct gcctggttgg cataacgacg gatcaaatgc cagaacgcct
     2881 ggcgcgtcat gggcgctgca cgtgaggtca caaataacgc gtctgcttgc ctgccgacca
     2941 aaatatccgg acgagccgca tggagatagc gctcgatcca ggagagtgcc tcctccccta
     3001 ccggcaccaa tcgttccttg ccgcctttgc cgatgacgcg taccacaccc gcgtcccggc
     3061 tgagttgacc cagactcaag ctaacaagct cggatacacg cagcccgcta gcgtacagcg
     3121 tctccagcat ggctctgtcg cgcagaccga gtgccatgtc gatatttggt gcagccaaaa
     3181 gcgtatcgac atcttcctct gtcaacattt tcggcaaact gcgcgggagc ttgggggagt
     3241 ctacttgcaa ggtcggatcc gcgtcgatcc gcttctctcg ctgctgatag cggtagaaac
     3301 gtttcagact ggatagcaga cgtgctgccg aacgtgccct ggacttatgc ttgaacctgt
     3361 gtgccaggta atccagaaga tccatccgtt gcacttgcaa cagagatttg ccgcgggttg
     3421 cagcgatcca cgtggcgaaa agcgtcaagt cgcgccgata gctttctagc gtattacgcg
     3481 caagaccatc ctctagccac agtgcatcgc taaacccatc cagcaatgct tcgtcggata
     3541 tcaccttcca gcaccccgct tatcgtggtg caaccaccat gcacgccgcc ctgtgcccat
     3601 cacggaatca agcgacgttc tgaaccttga ccctaatgtg ccatttttat gtgatgacat
     3661 tgtagcaatc cgatctaacc gcagggagcg gtcgaagttg gctatacttt gatttcaaca
     3721 gcgtctgcac aaaggtggca cctcaagtga ctgagcttga tcaatcgacc atttccatct
     3781 gcagcttcga agcgacgcaa cttccccctg gcgaacggct gcaaatggag attggttctg
     3841 acggtaatca gtactacacc acactgatcg gcttcgtacc gggacatagc gtgctggtac
     3901 gtacgccgct aatgcaaaat ttgccaatcc cggttccgga aggagctcgt gtgctggtga
     3961 gggcgttttc gggacggcac gcgtatacca tggaatcaca agtcgatcga gtgtgccgtt
     4021 cgccataccc ttacattcat attgcatatc ctgcccaagt ccagcaaaca cttatacgcg
     4081 gggcattacg cgttcgagtt gcgcttccgg gtgttgcttc caatgcgatt gagtcggaca
     4141 tatcgattac gcgtgcagta gtactttccg atctgagcac ttccggcgct caactggagt
     4201 cagaacaaag tcttggtgat ccgggcgaaa agtttcggct tgctttcaag ttcgtggtac
     4261 agccgaacaa ttatgaagtg aagctcgcca cttccgcaca gatccagagc gtgcgaaaga
     4321 cgaagaggaa aaacagtcag gaagagattt tttccaacgg agtgcgcttc aacaagcttc
     4381 acgccaccga aggactgctt ttgcaaagct acatccaaca ggttttactg gctgaccgca
     4441 accgcgtgat ataggtgtgc ggcacaaaaa aggcgactct aaggtcgcct ttttcatcca
     4501 ggaagacggt aactattcct ggtccgttgc tttttcactc tcaagctttt cacgaatgcg
     4561 tgctgacttt ccggaacgat cgcgaagata atagagcttt gcgcgtcgca catcgccacg
     4621 gcgctttagt tcgatgctgg cgattaaggg cgagtaagtc tgaaacgtac gttcaacgcc
     4681 ttcgccagaa gacatcttgc gtacgataaa tgaagaattc agtcctttgc tccggatcgc
     4741 aataacaacg ccttcgtatg cctgaacgcg tttgcgatct ccttccacga cattcacgtt
     4801 cacgatcacg gtatcgccgg gcgcaaatag aggaatatct ttccccagtc gcttgatttc
     4861 ttcctgctct atcttctgga ttaggttcat ttgatgtgct cctgcgcttt cttcgattct
     4921 ggtttgtatt cttccatctt gtattcttca agcaacgcca tttgctcggg gctcaatgat
     4981 aagttcacca acaaatcagg gcgcacgcgc caggtgcgtc ccaaagactg cttcatgcgc
     5041 cagcgcgaga tatgcccatg atgtcccgat aacagaacgg cgggaactgc atcgcgtccg
     5101 tacacttctg ggcgcgtata ttgcgggcaa tccagcaatc cattcgaaaa cgaatcctgt
     5161 tgtaccgatt cggcatcatt cagcgcgccg ggaatccttc ttataagcgc atccatcaat
     5221 accatagcgg gcaattcgcc accggacaat acatagtcgc cgatggaaat ctgatcatca
     5281 atttcagtct gcagcacccg ctcatccaca ccttcatatc gtcccgccag cagtaccaga
     5341 ccgtcgtgct ttcccagttc ctcgacaatc tgctgcgtca atggccggcc ttgcggggaa
     5401 agaaagacgg tgcggccatg ctttacgccc gaactggact gcctttgccg agccgcccgc
     5461 aacgccttgg cgagcggctc gaccatcatt accattccgg gaccgccacc ataaggcctg
     5521 tcatccacgc ttcgatacgc attggtgctg aaatctcgtg gattccacaa caccaactcg
     5581 taaagcccgc tgctgcgagc ccgctgggta ataccgcttc ccgtcaatgc a
//
LOCUS       DATMZQ010000213         4377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4570047, whole genome shotgun sequence.
ACCESSION   DATMZQ010000213 DATMZQ010000000
VERSION     DATMZQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4570047"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VN496_06925"
     CDS             <1..420
                     /locus_tag="VN496_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF16719.1"
                     /translation="QPALDTKPPESANAAHRILVVDDNRDSAESLAILLRLAGNDLQL
                     AYDGVEAVEKAAVQNPDIIVLDIGLPKMNGYDACRAIRAQPGGKDITIIAASGWAREE
                     DRRMSKEAGFDAHLIKPVDHATLMKLVTDAAARAPNR"
     gene            complement(434..1531)
                     /locus_tag="VN496_06930"
     CDS             complement(434..1531)
                     /locus_tag="VN496_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HXF16720.1"
                     /translation="MIYVAAVPLQKLVTEIFAATGCSLAEAERIARYLVRANLAGHDS
                     HGVVRVPRYVHLKHEGMIHSDRKVDVLIDTPVLAQVDGKFGFGQTVAPQAVEIGIAKC
                     KQMGLSAIALRHAGHIGRVADWAEMAAEQGLISIHFVNAAAGVLVAPFGSVERRFSTA
                     PYCVGVPVPGRFPLILDFATSIVAEGKVLVASQGGKAIPADALISADGHTSDDPRLLY
                     GDYKSGERRDIRKGSGALRAFGDHKGSGLALMCELLGGALTGNGCASPERPFSNGMFS
                     LYVDPVRIDPEGLFPEEVMRYVAHVKAAKTAAPLTETLVPGEPEERTRRTRSVQGIPL
                     SEETLSVLLETARSVGVEERIDGVPALPIAK"
     gene            1607..2401
                     /locus_tag="VN496_06935"
     CDS             1607..2401
                     /locus_tag="VN496_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXF16721.1"
                     /translation="MRNNNVRSIWKRGGAVINGWLGIPSSIAAENMAQANWDALTVDL
                     QHGHVDYQTAVTMLQAVSTTQTTPFARVPWMEPGIIMKLLDAGAFGIVCPMINTRADC
                     EAFVGACRYAPAGYRSFGPVRATWYGGADYFKYANDTIVAMAMIETRQAMDNLDDILS
                     VPGLDALYIGPNDLAISLGYAPSGVPTDPVVLDAIKTIAAGGKKHGIVSGIHCGSTAM
                     AKQMIALGFQFVTLLADNAFLSAAAKSAVAEMREPTAVASKATGTY"
     gene            complement(2420..3394)
                     /locus_tag="VN496_06940"
     CDS             complement(2420..3394)
                     /locus_tag="VN496_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXF16722.1"
                     /translation="MLRFPLVRALAVWGLVSISLFAADEFPNKPVRIIVPFGPGSGSD
                     VRTRQLVPLLSQRLKQPIIVDNRPGAAGSIGTQLAARSSPDGYTVTYILIGTVCINPH
                     LDQQTAFNPLRDLVPLIVTMRTALIMAVKADSPIRSVQDLIKQAKANPDRFNYGTSGP
                     GSVQHLIGEQFKKKAGVEMLAIPYKGGDGTTLADLMGGQIDIAFASPLTTMPLVEGGK
                     LRALAVTSAKRLANLPNVPTIAESGVPGYDETVWSGYAVPAGTPDAIVKKLHSAFQSA
                     MLSPEFREDITQSGAELIASSQQYAAQLLKADYERYGKIVKDLGLKAD"
     gene            complement(3436..4236)
                     /locus_tag="VN496_06945"
     CDS             complement(3436..4236)
                     /locus_tag="VN496_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16723.1"
                     /translation="MRLAGKVAIVIGAGQSPGEGMGNGRATASRFAQEGAKVLAVDRN
                     IDSANETAALAAKDGAECLAFEADVTSEATLADAVEFARRRWGRIDILHNNVGVSIAG
                     GDTELLELTESTFDRINAINLRGTVMACKHVIPVMRKQRSGVVLNISSIAAWADYPSV
                     VYKATKAAMIAFTQQLALQNASYGIRANVILPGLMDTPMAVDTRARATGETRMSIAAK
                     RNERVPLRGKMGTAWDVANAALFLASDEANFITGVALPVDGGALVRIG"
BASE COUNT          936 a         1296 c         1235 g          910 t
ORIGIN      
        1 caacctgcgt tggatacgaa accacccgaa agtgccaacg cggcacatcg cattctggtg
       61 gtcgatgaca accgcgattc cgctgaatcg ctcgccatct tgttgaggct ggctggcaac
      121 gacttgcagc tggcatacga cggggtcgaa gccgttgaga aagcggcggt ccagaatcct
      181 gacatcatcg tcctggacat cggtctgccg aagatgaatg gctacgacgc ctgccgtgct
      241 atccgggcac agcccggcgg caaggacatc accatcattg cagcgagcgg ttgggcacgc
      301 gaagaagatc gccgcatgtc caaggaagcc ggcttcgacg cacatctgat caagccggtg
      361 gaccatgcca cgctcatgaa actggttacc gacgcagcag cccgggcgcc gaacagataa
      421 tcttgccagc gctttacttg gcgatgggta gcgctggcac tccgtcgatg cgctcctcga
      481 caccaactga gcgggccgtc tcaagcaata cggacaaggt ttcctcggac agcggtatac
      541 cctgtacgga gcgggtgcgt cgcgtgcgct cttcaggctc gccggggacg agggtttccg
      601 ttagcggagc agcggttttc gctgccttta cgtgcgcgac atagcgcatt acctcttcgg
      661 gaaagagtcc ttccggatcg atgcgcactg gatcgacata caaggaaaac attccgttgg
      721 aaaaaggccg ttccggggaa gcgcaaccgt tgccggtaag cgccccgccc agcagttcgc
      781 acatcagcgc gagaccggac cctttgtggt caccgaaagc gcgaagtgcg cccgaacctt
      841 tgcggatatc tctgcgctcg cccgatttgt aatctccgta cagcaaacgg gggtcgtcgc
      901 tggtgtggcc atccgcgctg atcaacgcat ccgctggaat ggccttgccg ccctggctgg
      961 ccaccagcac ttttccttcc gctacgattg acgtggcgaa atcgagtatc agcgggaaac
     1021 ggcccgggac cgggacgcca acacaatacg gagcagtgga aaagcgcctt tctacactgc
     1081 cgaacggtgc caccagtaca cccgcagcgg cgttcacgaa atgaatggat atcaaacctt
     1141 gttcagccgc catctccgcc caatcggcaa ctctgccgat gtgtccggcg tggcgcaagg
     1201 ctatcgcaga aagacccatt tgcttgcatt ttgcgatgcc gatctcaacg gcctggggtg
     1261 ctacggtctg gccgaatccg aacttgccat cgacctgtgc gagcacgggc gtgtcgatca
     1321 gcacgtcgac cttgcggtca gaatggatca tcccctcatg cttcaggtgc acataacgcg
     1381 gtacgcgcac aaccccgtgg ctgtcgtgac cggcaagatt ggcacgcact agataacgcg
     1441 caatgcgctc cgcttcggcc agcgaacagc cggttgccgc gaaaatttcg gtgaccaact
     1501 tttggagggg aacggcggca acgtatatca tgccgcatca tacctttgcc cgcaggcttc
     1561 ggcagggtcg aatgatgtcc gtataccaac ataaaaggcg ctgaccgtga gaaacaataa
     1621 tgtgagatcg atctggaaac gcgggggagc agtgatcaac gggtggctgg gcattcccag
     1681 ttcaatcgca gcggaaaaca tggcccaggc taactgggat gcgttgactg tcgaccttca
     1741 gcacggccat gtcgattacc agacggccgt cacgatgctg caagccgtga gtacgaccca
     1801 aaccacaccg tttgcgcgcg ttccgtggat ggaacccgga atcatcatga agctgctcga
     1861 tgccggcgcc ttcggcatcg tctgcccgat gatcaatacg cgcgcagact gcgaggcgtt
     1921 tgtcggagca tgccgttacg cgcccgccgg ctaccgcagc tttggtccgg tacgggcaac
     1981 ctggtatggg ggcgccgatt atttcaaata tgccaacgac accatcgtcg cgatggcaat
     2041 gatcgaaacc aggcaggcga tggacaatct tgatgacatt ctcagcgtgc cgggacttga
     2101 tgcgctctac atcggcccga acgatttggc catctcactg ggctacgccc cgtcgggcgt
     2161 gccgacagat cccgtcgtcc tcgacgcaat caagaccatc gcggcaggcg ggaaaaagca
     2221 tggaatcgtg tctggaattc attgcggatc caccgcaatg gccaagcaaa tgatcgcgct
     2281 gggcttccag ttcgtgacct tgcttgcgga caatgcattt ctcagtgctg ccgcaaaaag
     2341 cgcggtggcg gaaatgcggg agccgactgc ggtggccagc aaggcgacgg gtacgtatta
     2401 gcaccagacc cgagcagatt tagtccgctt taagtccaag atctttgacg attttgccgt
     2461 atcgctcgta atcagctttt aacaactgcg cggcatactg ctggctactc gcaatcagtt
     2521 ccgcgcccga ctgtgtgatg tcctctctaa actctggaga aagcatggca ctttgaaagg
     2581 cgctgtgaag ttttttgaca attgcatctg gtgttccagc aggaactgcg tagcccgacc
     2641 aaacagtttc atcgtagccg gggacacccg attcggctat ggtgggcacg ttcggcaggt
     2701 tggcaagtcg cttggctgaa gtcactgcca acgcacgaag cttgccgcct tccaccaaag
     2761 gcatcgtagt caacggagag gcaaaagcaa tgtcaatttg cccccccatc aaatcagcca
     2821 gagtagtccc gtctccacct ttatagggta ttgcgagcat ttcaacgcca gccttttttt
     2881 tgaactgttc ccctatcaaa tgctggaccg agccagggcc gctcgtccca taattaaatc
     2941 gatccgggtt tgctttcgcc tgtttgatca agtcctgcac ggaacgaatt ggcgaatccg
     3001 ctttaacagc cataatcagc gccgttctca tggttacaat caaaggcacc aagtctctaa
     3061 gcggattgaa tgcggtctgc tggtcgaggt ggggatttat gcaaaccgta ccgataagaa
     3121 tgtaggtgac ggtgtagccg tcgggggaag acctcgcagc cagttgggtg ccaatgcttc
     3181 ctgcggcccc aggtcgattg tcaacgatga tcggttgctt caaacgctgc gagagaagtg
     3241 gcacgagttg gcgcgtgcgt acatctgatc cggatcctgg cccgaacggg acgatgatcc
     3301 gaaccggctt attcggaaat tcatccgccg caaacaagga aatgctgacc agaccccaca
     3361 ctgccaacgc tcggacgagc gggaatcgca gcattgattt tatgcagcgg gcattagggc
     3421 acatcttgct cgcccttatc ctatccgcac cagcgcccca ccatccaccg gcagcgcaac
     3481 accggtgatg aagttcgctt cgtcggatgc aagaaacaac gcggcattgg cgacatccca
     3541 tgcggttccc atcttgccgc gcagcgggac tctttcattg cgtttcgccg caatcgacat
     3601 acgtgtctcg ccggtggcgc gagcccgcgt gtccaccgcc atgggtgtat ccatcaagcc
     3661 cggcaaaatg acgttggccc gaattccata gctggcgttt tgcaacgcaa gctgctgcgt
     3721 aaaggcgatc atggcggcct tcgtcgcttt gtacaccacg gaaggatagt cagcccacgc
     3781 agcaatcgaa gatatgttga gcacgactcc gctgcgctgc ttgcgcatga ccggaataac
     3841 gtgtttgcat gccatgacgg ttccgcgcag gttgatggcg ttgatgcggt cgaaagtcga
     3901 ttcagtcaac tccagcaact cggtatcgcc gcccgcaata ctgacgccca cgttgttatg
     3961 cagaatatca atccgtcccc aacgccgccg tgcaaactcc acggcatctg cgagcgttgc
     4021 ttcactggtc acatcggctt cgaatgcgag gcattctgcg ccatcttttg cagccagcgc
     4081 agcggtctcg tttgcggaat caatattgcg gtctaccgcg agcaccttcg caccttcctg
     4141 ggcaaagcga cttgcggtgg cgcggccatt tcccatgccc tctcccgggc tttgcccagc
     4201 accaatgacg atggccactt ttcccgctag tcgcataact tctcctgcca tcggggcgtc
     4261 gctaccggct tcggtttacc gcaacatcag attcggcgag ccccgacagc tttttctggc
     4321 cggggcgagg gggtcgccgt gaatttttta cccggcgcct gggggcggag tttgttg
//
LOCUS       DATMZQ010000214         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3962343, whole genome shotgun sequence.
ACCESSION   DATMZQ010000214 DATMZQ010000000
VERSION     DATMZQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3962343"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..2185
                     /locus_tag="VN496_06950"
     CDS             272..2185
                     /locus_tag="VN496_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF16724.1"
                     /translation="MPLATFENVSLAYGHVPLLDHVDLVVEPGSRIGLIGRNGAGKSS
                     LLRLLAGSALPDDGRIWRQPGLRIGVVAQEPELDPKQSVFEAIVAGLGELSRILADYH
                     SAAHQLQEEQEDSAQALERMQAAQELLERHDGWRVEHRVEAVLSRLGLAPDARIDTLS
                     GGLRKRVALARALAADPQLLILDEPTNHLDVDSIVWLENMVVEFPGAVFVVTHDRAFL
                     DRVVTGIAELDRGRLGIYPGTFAQYEQRKVEHLAVEAVNNQKFDKLLAQEEVWIRKGI
                     EARRTRNEGRVRRLERLRLERAARRERLGQVAIAVAEGERSGQLVAELEHVDKQLGGR
                     HIVRDFSCRILRGDKIGFIGANGAGKSTLLRLILGEIQPDAGSVRRGTKLSVAYYDQF
                     RAQLDEESTVAQTIAPGSDYVEIDGSRKHIMSYLGDFLFPPERARAPVKALSGGERNR
                     LLLARLFSQPANLLVLDEPTNDLDIDTLELLETLLQDYAGTVLLVSHDRAFLDNVVTD
                     TIAPEGDGRWTRNAGGYSDWRRLLEERAGHGVRATEGAKPPSRDRSVTQRPRKLGFKQ
                     ARELDEMPARIQALETEQGQLNARLADPLLYSKPASEIRQLQQRHAAIEDELTACLRR
                     WEELEALRDHAGA"
BASE COUNT          434 a          652 c          700 g          419 t
ORIGIN      
        1 tgccgcaagg gccgcgagtg gcccatgcgg aatgctggct tacgcgtggt aacacctctc
       61 tacgtgaccg aaggcatcga cggcattccg tagtcacggc attcacgata ttcacgatat
      121 tcacggcatc cgcggtattt cgtcgtgacc taaggcattg acggcattcc ctagtcacgg
      181 cattcacgac attcacgata ttcacggcat tcacggtatt tcgtcggtta tgattccgcc
      241 tcttttcaat cctggccgaa ccggctagct aatgcctctc gccactttcg aaaatgtttc
      301 gctcgcgtac ggccacgtgc cgttgctgga tcatgtcgat ctggtggttg agcccggcag
      361 ccgcatcggg ctgatcgggc gcaacggcgc gggcaaatcc agtctgctga gattgctggc
      421 cgggtcggca ctgcccgacg atggacgcat ctggcgacag ccgggactgc gcatcggcgt
      481 ggtcgcgcag gagcctgaac tggatccgaa acagtcggtg ttcgaagcga ttgttgcggg
      541 gctgggtgag ctttcccgca tactggccga ttaccattct gccgcacacc aactgcagga
      601 agagcaagaa gactcagcgc aggcgttgga acgcatgcag gctgcgcagg aattacttga
      661 acggcacgac ggctggcgcg tcgagcaccg cgttgaagcg gtgctctcgc ggctgggcct
      721 cgcgccagat gcccgcattg acacattgtc tggtggtctg cgcaagcgcg tcgcgctggc
      781 gcgcgcgctg gccgccgatc cacagctgct gatcctcgac gagccgacca accatctcga
      841 tgtggactcg attgtctggc tggagaacat ggttgtggaa tttcctggcg cggtattcgt
      901 ggtcacgcac gatcgcgctt ttctcgaccg cgtggtgacc ggcattgcgg aactcgatcg
      961 cggccggctg ggcatttatc ccggcacgtt cgcgcaatat gaacagcgca aggtggaaca
     1021 cctcgcggtt gaagcggtga acaaccagaa gttcgacaag cttcttgcgc aggaagaagt
     1081 ctggattcgc aaaggcatcg aagcgcgccg aaccagaaac gaaggccgcg tgcggcggct
     1141 ggagcggctg cgcctggaac gcgcggcgcg gcgcgaacgc ctcggccagg ttgccattgc
     1201 cgtagcggaa ggcgaacgat cgggtcagct ggtggccgag ctcgagcatg tcgacaagca
     1261 gctgggcggc cgccacatcg tcagggattt ttcctgccgc atcctgcgcg gggacaagat
     1321 cggcttcatc ggcgcgaacg gcgccggcaa gtcgacttta ctcagactga tcctgggcga
     1381 gatccagcct gatgcaggca gcgttcgacg cgggacgaaa ctctcagtag cctattacga
     1441 tcagtttcgt gcgcagcttg acgaagagag cacggttgcg caaaccatcg cgccgggctc
     1501 cgactatgtc gagatcgatg gctcgcgcaa gcacatcatg agctacctgg gcgattttct
     1561 gttcccgccc gagcgggcgc gtgcgcccgt gaaagcacta tcgggtggtg aacgcaaccg
     1621 gctgctgctc gccagattgt tcagccagcc ggcgaacctg ctggtgctcg atgaaccgac
     1681 caatgatctc gatatcgaca cgctcgagtt gctggaaacg ctgctgcagg actacgcggg
     1741 cacggtattg ctggtgagcc atgaccgcgc ttttctggac aacgttgtta ccgacacgat
     1801 cgctccggaa ggcgacggcc gttggacccg aaatgcgggt gggtacagcg actggcggcg
     1861 tttgctggaa gagcgtgcgg gacacggcgt gcgcgcgacg gaaggcgcca agccgccatc
     1921 acgcgatcgt tctgtgacac agcggccacg caagcttggt ttcaagcagg cccgggagct
     1981 tgacgaaatg ccggcacgca tccaggctct ggaaacagaa cagggccagc ttaacgcccg
     2041 cctcgccgat ccgttgctgt acagcaagcc agcaagtgaa atccggcaat tgcagcagcg
     2101 ccatgcggcg attgaagatg aactcactgc ctgcctgcgt cgctgggaag aactcgaagc
     2161 cctgcgtgac cacgcaggcg cttgatgtcg cgatgactca gctta
//
LOCUS       DATMZQ010000215         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_166133, whole genome shotgun sequence.
ACCESSION   DATMZQ010000215 DATMZQ010000000
VERSION     DATMZQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166133"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="VN496_06955"
     CDS             complement(<1..1879)
                     /locus_tag="VN496_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXF16725.1"
                     /translation="MTFSRYQLLGSLLNERILILDGAMGTMIQAHGLDAQHYHGERFL
                     AHGCDQKGNNDLLTLTQPQVIGGIHRAYLEAGADIIETNTFNSNAVSMADYGMTELVY
                     ELNSAGGRLARDAADEFSSLTPALPRFVAGVLGPTSRTASISPDVHDPGARNVSFDEL
                     VACYAQCVRGLVDGGVDLLMVETIFDTLNCKAALFAIDEYCEQHSVQIPVMVSGTITD
                     ASGRTLSGQTTEAFWNSISHARPFSVGLNCALGAKELRPYIEELSAIADVHTSLHPNA
                     GLPNSFGEYDETPEYTASFLEEFAKAGFLNIAGGCCGTTPAHIRAVAQALKGIAPRRV
                     PTIDKKLRLSGLEPLNIGDDSLFVNVGERTNVTGSKAFARLILAGNFSEALVVARSQV
                     DNGAQIIDVNMDEAMLDSRKAMTTFLNLIGSEPDISRVPVMIDSSKWDVIEAGLKCVQ
                     GKCVVNSISMKEGEEEFLRQARLVRRYGAAAVVMAFDEKGQADTLARRIEICKRAFEL
                     LVHQANFPPEDIIFDPNIFAIATGIEEHSNYAIDYIEATRAIKAALPHVKISGGVSNI
                     SFSFRGNDPIREAIHTAFLFHAIKAGMTMGIVNAGQLGVYADIPKDLLERVEDVIFNR
                     RA"
     gene            complement(2049..>2894)
                     /locus_tag="VN496_06960"
     CDS             complement(2049..>2894)
                     /locus_tag="VN496_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXF16726.1"
                     /translation="PPGTGKTMLAERFAGILPPMTEDEALESAAMQSLGSTGFDVRRW
                     KCRPYRSPHHTASAVALVGGGSNPRPGEISMAMHGVLFLDELPEFNRDVLEVLREPLE
                     SGKISVSRAARQAEFPARFQLLAAMNPCPCGFLGHYSGRCHCTPDQVTRYRNRISGPL
                     LDRIDLHIEVPAVADKEIAVRQPGERSAAIRDRVAVARERQLTRQGKPNANLSVKEIG
                     EQCIVDGEADLLARKAIGTLGLSARAYHRILKVARTIADLAGVSSIFAPHVAEAVQYR
                     RYERI"
BASE COUNT          617 a          848 c          826 g          603 t
ORIGIN      
        1 cggctcggcg attgaaaatc acatcttcca ccctctccag caggtccttc gggatgtccg
       61 catagactcc gagttggccg gcattgacaa tgcccatggt catgccggcc ttgatggcat
      121 ggaacaggaa tgcagtgtgg atcgcttcgc ggatcggatc attgccgcga aatgagaacg
      181 aaatgttgga gactccgccg cttatcttga cgtgaggcaa cgccgccttg atggcacgcg
      241 tggcctctat gtagtcaatg gcgtagttac tatgctcctc gatgccggtc gcaattgcga
      301 agatgttcgg atcgaaaatg atgtcttccg gtgggaagtt ggcctgatga accagcagct
      361 cgaacgcgcg cttgcaaatt tcgatgcggc gggccagcgt atcggcttgg cctttttcgt
      421 cgaaagccat gacaacagcc gcggcgccat agcggcgcac cagacgcgcc tgacgcagaa
      481 attcttcttc gccctctttc atgctgatcg agttcaccac gcacttgccc tgcacgcact
      541 tgagcccggc ttcaatgacg tcccactttg aagagtcgat catgaccggc acgcgcgaaa
      601 tgtccggttc cgagccgatg aggttcaaaa aggtcgtcat ggcctttctg gaatcaagca
      661 tcgcctcgtc catgtttacg tcgatgatct gggctccgtt gtccacctgg ctgcgcgcca
      721 cgacgagtgc ttcactaaag ttgccggcca ggatcaagcg cgcgaatgct ttggagccag
      781 tgacattggt tcgctcaccg acgttcacga acaaggaatc gtccccgata ttcaggggtt
      841 cgagaccgga aagccgcaat ttcttgtcga tggtcggcac ccggcgcggc gcgatgcctt
      901 tcagtgcctg agcgacggcc ctgatatgcg ccggcgtggt gccacagcag ccgcccgcga
      961 tgttcagaaa cccggctttt gcaaattcct ccaggaacga tgcggtgtac tcgggcgtct
     1021 cgtcatattc gccaaatgaa tttggcaggc cggcattcgg atgaaggctg gtatgtacat
     1081 ccgcaatcgc tgataactcc tcgatgtaag gacgcagctc tttcgcaccc agcgcgcaat
     1141 tgaggcccac ggaaaagggt cgtgcatgcg aaatcgaatt ccagaatgcc tctgttgtct
     1201 ggccggaaag ggtgcggcca cttgcgtccg ttatggtgcc ggacaccatc accggaattt
     1261 gcacgctatg ctgctcgcaa tactcatcga tggcgaacaa cgcggcttta cagttcaagg
     1321 tgtcgaatat ggtctccacc atgaggaggt cgacaccgcc gtccaccagg ccgcgaacgc
     1381 attgcgcata acaggcgacc agctcatcga atgagacgtt tcgcgcaccc gggtcatgga
     1441 cgtccggaga aatcgaagcc gtccggctcg tcggacccag cacgccggct acgaagcgcg
     1501 gcaaggccgg agtaaggctg gaaaattcgt ccgcggcatc gcgcgcaagc cgaccaccgg
     1561 cgctattcag ttcatatacc agctcagtca tgccgtagtc ggccatcgac acggcattcg
     1621 agttgaaggt attggtttcg atgatgtccg caccagcctc gagataagcg cggtggatac
     1681 cgccgattac ctgaggttgc gtcagggtca acaaatcatt gttgcccttt tgatcgcatc
     1741 cgtgcgcaag gaagcgttct ccatgatagt gctgcgcgtc gagcccatgg gcctgaatca
     1801 tggtgcccat tgcgccatca aggatcagaa tgcgctcgtt gagcaaactg ccaagcaatt
     1861 ggtagcgcga aaatgtcatg gcttacccaa ataaaaaccc gtctgcctga gcgggacggg
     1921 tttgtcacgc tttagcggca tttattaagc gcccgcaagc tgagtgtcaa atcggcgcga
     1981 tgacaaatta ccgtgaaaca gggcaatgag tcaatgcgct ggccagcgca gcgatgcatt
     2041 gcgttccgtc aaattctctc gtaccgccgg tactggacgg cctcggcgac atgtggcgcg
     2101 aaaatgctgg aaacacccgc cagatccgca atagtgcggg caactttaag aatgcgatga
     2161 taggcacgcg ccgataaacc caatgtgcca atcgccttgc gagcaagcag gtctgcctcg
     2221 ccatcgacga tgcactgttc tccgatctcc ttgaccgaca ggttggcatt tggtttcccc
     2281 tgccgcgtca gttgccgctc gcgcgccact gccacgcggt cacgtatagc ggcactccgt
     2341 tctccgggtt gacgtacggc gatctctttg tccgctacgg ccggaacctc gatatgcaag
     2401 tcgatgcggt ccagcagggg gccggatatg cgatttcgat agcgcgttac ctggtctggc
     2461 gtgcaatgac aacggcctga atagtgaccg aggaaaccgc atgggcacgg attcatggcc
     2521 gcgagcaact ggaaacgtgc cgggaattcg gcctgtctgg cggcccggga tacgctgatt
     2581 tttcccgact ccagcggctc ccgcagcact tcaagcacat cacgattgaa ttcaggcaat
     2641 tcgtcgagga atagcacgcc atgcatcgcc atcgaaatct cccccggacg aggattgctg
     2701 ccgccaccga ccaacgccac ggcggatgcg gtatggtgag gagatctata cggccgacac
     2761 ttccagcggc ggacatcgaa acctgtgctg cccaaggatt gcatcgccgc agattccagc
     2821 gcctcgtctt cagtcatggg cggcagaatg ccggcaaaac gttcggccag catggtcttc
     2881 cccgtgcccg gcgg
//
LOCUS       DATMZQ010000216        14564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_514378, whole genome shotgun sequence.
ACCESSION   DATMZQ010000216 DATMZQ010000000
VERSION     DATMZQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514378"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN496_06965"
     CDS             <1..185
                     /locus_tag="VN496_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16727.1"
                     /translation="LKKDRQRDLNTMQVPRFALPRAPSSHATFLGVRHSYHRKAKAAS
                     WSEIQSGMISLPSPPR"
     gene            258..1289
                     /locus_tag="VN496_06970"
     CDS             258..1289
                     /locus_tag="VN496_06970"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXF16728.1"
                     /translation="MWNTLKGWLGGLLSHARELFLRLADRPVGRWVEGSYAESNNRVF
                     FFLPQSKRTYHLYLPRRYSATRAAPLVVMLHGCKQDAASFASGTRMNELADQEGFVVL
                     YPEQKRMANAQRCWNWFDSSAQRGHGEAALIAGMVREVLRSHAIDPSRVYIAGLSAGG
                     AMTSIMASCYGNLFAACAIHSGLMYRAAESVSQASRAMQHSSRASAQEAARHAMAHET
                     FDFVPVLVIHGTADSVVNPVNADQVVEQFIAMGTPTRVNGAPVAEFASSKAAGGRRGF
                     EIRDYRSGDLLLTRKILVHGLDHAWSGGNGAHPFNDPFGPDASRMIWDFFSEFQRSEL
                     KNNVAANFK"
     gene            1426..2229
                     /locus_tag="VN496_06975"
     CDS             1426..2229
                     /locus_tag="VN496_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HXF16729.1"
                     /translation="MVKAKISPIQKTAFKARALLIGERISLKSWGHIDSLATNPLTVE
                     VKGGGAATLHRYGVAVFFDVSPVEEIAFLANLQPSVSNLYAMPETEEVEVRVEPGVRE
                     IIKGGVAYLEDAAVERLQVIADILSKSVLLSLYEKSVGVEFDRIEPLAIELDKRGRIG
                     GKSKDLLKKIGSMLLVEQRMVGRAEITEKPEMLWDNADLEGLFARLEDEFEIKERHLA
                     LERKLNLISDTAHTLLELMSSEHALRLEWYIVILIAVEILLTVYQLFLS"
     gene            complement(2276..3400)
                     /locus_tag="VN496_06980"
     CDS             complement(2276..3400)
                     /locus_tag="VN496_06980"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HXF16730.1"
                     /translation="MRMTRTVIALFLVCTAVHAYESGSGEAAASARYTFTWPLGAGPE
                     PRGASTKGVSVTLDQEPAEAWKRLHEAGLSDLERDRRAILAMAGPYRVNFDFLEVVRF
                     DPSLKPDAPYQSWGTEYVFVSQDSGDFIALQHILVMRVLQQDGTPSEPLVTRHWRQEW
                     QYQAETALVYTGADTWMRKPIPPDQRRGAWVQSVYQVDDSPRYASSGRWLHTDSFSTW
                     NSDETWRPLPRREWSVRKDYQALIGTNRHTIMQTGWLQEENNLKVVLNANGKPRAVLP
                     FLAREYGVARYERIKNYDFDAGKDYFEKTEPFWSEVRAAWREVASAGRFKLSKPVDQG
                     QLFTPFFDYAEKLAEGESFVREDARAFIRQTLHSRYLAPA"
     gene            3594..3773
                     /locus_tag="VN496_06985"
     CDS             3594..3773
                     /locus_tag="VN496_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16731.1"
                     /translation="MMKFNATLKALVLIVAIAGLSACQKGPAEKAGEKIDNAAKDVGN
                     KMEHAGDKIKDAVKK"
     gene            complement(3810..4478)
                     /locus_tag="VN496_06990"
     CDS             complement(3810..4478)
                     /locus_tag="VN496_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXF16732.1"
                     /translation="MDARKASLADSVYLGVKQNIFDFRLLPGDRFTENEIAARLHVSR
                     TPVREALYRLEREGYLQVQFRSGWSVRELDFTQFDDLYDLRIALESACVQTVCARAEL
                     PALDDLKSIWLIEPKRRLDDGEQVCALDEAFHGALVAAARNREMARCHQEVTERIRLL
                     RRLDFTEASRIAATYEEHAQILRAVLTRRADRAIVLLRKHIETSKTEVRKISVHKLLS
                     ARRR"
     gene            complement(4547..6337)
                     /gene="atzF"
                     /locus_tag="VN496_06995"
     CDS             complement(4547..6337)
                     /gene="atzF"
                     /locus_tag="VN496_06995"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HXF16733.1"
                     /translation="MTISSLLADYRQGKRAPSEVIESLGSARDGIYASAWICRVEHDM
                     LVRRARQLETLASADANALSRMPLYGIPFSVKDNIDVAGLPTTAACPAFSRVPERSAR
                     AVELLEAAGAIMIGKTNLDQFATGLVGTRSPYGPVPNSFRPEYISGGSSSGSAVTVAR
                     GVVSFSLGTDTAGSGRVPAGLNNIVGLKPSRGLVSARGVVPACQSLDCVSVFALTVAD
                     AVAVLRVMRGYDAEDPWSRQLTGEAPRLPERFTFALPEHLEFYGDAHAMHAFNEAVDR
                     LEGLGGKPIRTDFSPYLRAAALLYEGPWVAERLAAIRSFFEQHADQVHPVIRKIIEGA
                     SDYSAADAFAGLTRIAAMKKATAQLWNRADVMVVPTAPTAYTVAEVLAEPFQTNRRLG
                     YYTNFVNLLDLAAFAVPASMRPDGLPSGITFIAPAGSDLMLADLAQRFHQLTGLQLGA
                     LDETLPAAEPITIADHNVRVAVVGAHLSGLPLNYQLTERGGRLEKAARTSANYRLYAL
                     PGTTPPKPGMVRDPGLGRAIDVEVWRLPMHEYGSFVAGIPSPLGIGQIELDTGEWVQA
                     FICEAWATRGAEEISHLGGWRAFLARKAAH"
     gene            6447..7118
                     /locus_tag="VN496_07000"
     CDS             6447..7118
                     /locus_tag="VN496_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HXF16734.1"
                     /translation="MDKMAIEAEPYELEFNPKNTALLIIDMQRDFVMPGGFGEALGND
                     VTPLQATIAPTARVLSAARKRGMLVVHTREGHRPDLTDCPPSKLVRGRGKTRIGDHGP
                     MGRILVRGEVGHDIVPELYPRSGEPVIDKPGKGAFYATDLELILRDRAIKTLIVCGVT
                     TEVCVNTTVREANDRGYECVVLSDCVGSYFPEFQKFALEMIKAQGGIFGWVSDSTRAL
                     AAIGI"
     gene            7138..8127
                     /locus_tag="VN496_07005"
     CDS             7138..8127
                     /locus_tag="VN496_07005"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HXF16735.1"
                     /translation="MPAGPTDTQAWRAESIGDAVPLRVSGTVHSVFAHACNIETASGD
                     LVTLLASTQGNGPRGIKLAHAAALLYARLSAGQEVLIGQALLRVPAAAVAIDFAGARV
                     WSGEIAAVSPNVCARALRRVRSILCERVFLQGFVPLIAGSRPAQSAFDRAFIERLSST
                     LPALAQATVRHDSVEVAGIAAQLVGLGPGLTPSGDDFLTGYLAALWSRAGTDGGIAAL
                     LTRIDVSLTPLFLRTNAISRQMLRDAVQGRFAEHLLDVISALAHAGDVVHATMRALES
                     GHSSGADTLCGLLFGYAPHMAIPSVSGPLMMRSKLGVGALAWINGESACPLSW"
     gene            8109..9698
                     /gene="fdrA"
                     /locus_tag="VN496_07010"
     CDS             8109..9698
                     /gene="fdrA"
                     /locus_tag="VN496_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HXF16736.1"
                     /translation="MSSLLVKIVPREYRDSVSLMQLSAMLGKMPGIEQASAVMATENN
                     LALLAEAGLRVDAKGANPSDLLIVVQGDKAELPAAIAEAMKRLTRQGEDNAGEARAAL
                     RPRSIQMALKLAGESNMALISTPGDYAGAEAMKALRLGLNVMLFSNNVSVEEELALKQ
                     FARERELIVMGPDCGTAIVNGISLAFANVVRRGPVGAIGASGTGLQQVTVLVHRLGSG
                     ITQAIGTGGRDLSKEIGGISMLKGLKDLAADAATQVIVLISKPPAPEVAERVLAQAAK
                     AGKPVVVVFLGADAKSIARKGVHAVNTLEDAARAAVAIVKGDKPRESYADVPDLAVGL
                     PKFAESQRYIRGLYSGGTFCYEATLLLGALLPEVYSNTATRNAHELADVWQSHAHTLV
                     DLGDDVFTRGRPHPMIDYRLRTERIVKEAEDPETAVILLDVVLGYGSHMDPSSELVPA
                     IQRARAIAATQGRAVSFVGHICGTASDPQDLGRQTRALSEVGMLLAESNAQAVRLAAA
                     IAGKNGPLQRANTAEKETRLR"
     gene            9695..11128
                     /locus_tag="VN496_07015"
     CDS             9695..11128
                     /locus_tag="VN496_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HXF16737.1"
                     /translation="MSALFGEGQSIVNVGLQSFADSIVAGGGKAVQVDWTPPAGGNRV
                     AGWSLAQLLNHPDVERANETAFAAYLAAQPVLVGIGVAGEQLPDMKARMILHAGPPID
                     WQDMCGPMQGAVIGAILYESWANDADGARRLAEDGAIAFAPAHHHAAVGPMAGVISPS
                     MPVWVIENAAGSNRAYSNLNEGLGKVLRFGANSGEVIKRLRWMAEVLAPALRAALTTF
                     GPLELKPLIAQALQMGDEVHNRNAAASSLFLKRIIPAALHSGFDAGAIAEAVTFIAGN
                     DHFFLNLSMAACKVMLDAAHGVKGSSLVTAMSRNGVRFGVRLSGTGAQWFEAPSPMVG
                     GLFFPSYGPEHAAPDMGDSSITETAGLGGFAMATAPAIVQFVGGTPQDAIANTREMHY
                     ITLGRNSAFTLPAMNFGGTPAGIDARRVLDTNIMPIINTGIAHKEAGIGQIGAGITRA
                     PLECFTQAVVALASRLDVAVPGSIPAS"
     gene            11155..12108
                     /gene="arcC"
                     /locus_tag="VN496_07020"
     CDS             11155..12108
                     /gene="arcC"
                     /locus_tag="VN496_07020"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HXF16738.1"
                     /translation="MTKREHTAVVAVGGNALIIDEHHQSIPDQVKAATLTSHYVADMV
                     EAGWTVVLTHGNGPQVGFILRRSGIAKGEVPEVPVDFADADTQGAIGYMFQRALYNEF
                     QRRKLKRKVVAIVTQVRVDPRDPAFNAPTKPIGAHMTEDIAKALAQDLGWTVREDSGR
                     GWRRVVASPQPQEIIELGIIEALIDLGYIVIACGGGGIPVLRDGKGELQGLEAVIDKD
                     FASSLLARDLRAELFVVSTGVEKVAVNFGKPEQRWLERVGLDEARRHLADGQFPKGSM
                     GPKIEAVIAFLEGGGSRALITDPPNLGRALSGATGTHIVPA"
     gene            complement(12135..13709)
                     /locus_tag="VN496_07025"
     CDS             complement(12135..13709)
                     /locus_tag="VN496_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16739.1"
                     /translation="MSTAETAASSRMTAASAGNPSWWVQGDWNGFFGLFTNVLLNVIV
                     LTTLCLYVVQLPPDTVYGRVLPALGIALPIGNLFYAWLAYRLAKETGRSDVTAMPYGP
                     SVPHMFIVVLVVMLPTYLSTKDPMLAYAAGLAWCFIIGIIVLLGCFVGPWIRKMTPRA
                     AMLGTLAGISIAFISMRPAFQMWEVPWIGFVCFAIVLIAWTANIRLPGGIPGGLAAVV
                     LGALIGWIAAAFGWSDYMKGAEVSKAFQHFGLHLPWFSGDVFRGLAGIAPLLATAIPL
                     GIYNFTEAMNNVESASAAGDNYNLRKILAADGIGAIVGSMLGSPFPPAVYIGHPGWKS
                     VGGRIGYSFATGIAIALVCFLGLTALLLNLIPLVAILPILLYIGLVIGAQAFQASPSN
                     HAPAVVLAIIPNIAEWAKINIDGALGAAGTSADKVGMAALGQVGVVYQGLTVLGGGSV
                     LAGMVLGAMAAFIIDRKLAQAAVVAFVGAAFSFIGLIHGPQLGWMVSPLVALGYVMFG
                     AACLVLSRQDAPPVRA"
     gene            14259..>14564
                     /locus_tag="VN496_07030"
     CDS             14259..>14564
                     /locus_tag="VN496_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16740.1"
                     /translation="MKSFERHRIRLRQVLIMLLGALATGCGQGSSNSGPAVAHVAVTC
                     PVERINALEKAMDNTLSQANTEVDFSFAVEKKDGRRYVFNRGASTMQTAYQTASTSKM
                     "
BASE COUNT         3006 a         4278 c         4461 g         2819 t
ORIGIN      
        1 gtttgaaaaa ggatcgccag cgtgatctga acaccatgca ggttccgcgc ttcgctttgc
       61 cgcgtgcacc atcttcgcac gcaactttcc tgggggtgcg gcactcttac caccgtaaag
      121 cgaaagcggc gagctggagc gaaatacaat ccgggatgat atcgctgcct tcgcctccac
      181 gttagaatgt cagcaacgtc ttcatccatt caggcagaga acattttttg cttcgagcct
      241 gaaaccttcc aactttcatg tggaacactc tcaaaggctg gctggggggg cttctctccc
      301 atgcccgcga attgtttttg cggctcgccg accggcccgt tggccgctgg gtagaaggca
      361 gctatgcaga gtcgaacaac agggtctttt ttttcttgcc ccagtccaaa cgtacttacc
      421 atttgtattt gccacgcagg tattccgcga cccgagcagc gccgctggtg gtgatgctgc
      481 atggatgcaa acaggatgct gcgagctttg ccagcggcac gcgcatgaac gagctcgcgg
      541 atcaggaagg cttcgtcgtg ctgtacccgg aacagaaaag aatggcgaat gcgcagcgct
      601 gctggaattg gtttgattcc tctgcgcaaa gaggccatgg tgaagccgca ctgatcgcgg
      661 gtatggtgcg cgaggtcctg aggagccatg ctattgaccc tagccgtgtc tacatcgcgg
      721 gcctgtcagc cggtggcgcc atgacaagca tcatggcaag ctgttatggc aacttgttcg
      781 ccgcgtgcgc gattcattca ggcttgatgt atcgcgccgc ggaatcggtc tcacaggcga
      841 gccgcgccat gcagcatagt tcgcgcgctt ccgcacagga agcggctcgt catgccatgg
      901 cccacgaaac tttcgatttc gtcccagtcc tggtcattca cggcacggct gacagcgtgg
      961 ttaatccagt gaacgcggac caggtcgtcg agcagttcat cgctatgggc acgcccaccc
     1021 gtgtaaatgg tgcccctgtc gccgaattcg caagcagcaa ggcggccggg ggacgccggg
     1081 gttttgaaat tcgcgactac cgaagtggcg acctgctgtt gacgcgcaag attctggtgc
     1141 acggcctgga tcacgcatgg agcggcggaa acggtgcgca tcctttcaac gatccgtttg
     1201 gtccggatgc cagccgtatg atctgggatt tctttagcga attccagcgc agtgaactca
     1261 agaacaatgt tgcggcgaat ttcaagtgat caataaccgc gttggcgctc ctgctcgctc
     1321 tgccgaggca accacgaccc agtcgaacgc gtcaaagagc tttatctggc ctggcttttc
     1381 ctgctgcgca ccgccgcgct aaacaatcac gctatttccc cgtctatggt taaggccaaa
     1441 atctcgccga tccaaaaaac cgctttcaaa gcccgggcgt tgctgatcgg agaacgcatc
     1501 agcctgaagt cctggggcca tatcgacagt ctggccacca atccactcac cgtcgaagtc
     1561 aaaggcggag gcgcagctac gcttcaccgc tatggggtag cggtgttctt cgatgtctcg
     1621 ccagtggagg aaatcgcttt tctggccaat ctgcagcctt ctgtttcgaa tctttacgcg
     1681 atgccggaaa ccgaggaagt tgaagtgcgc gtcgaaccgg gcgtccgcga gatcatcaag
     1741 ggcggtgttg cctatctgga agatgcagca gtggaacgac tgcaagtgat tgccgatatc
     1801 ctgagcaaga gcgtactgct gtccctttac gaaaaaagcg tcggtgtcga gttcgatcgc
     1861 atcgaaccgc tggctatcga actggataag cgcgggcgca tcggcggaaa aagcaaagac
     1921 ctgttgaaaa aaattggctc gatgcttctg gtggagcaac gcatggtggg acgggcggag
     1981 attactgaaa aaccggaaat gctctgggac aatgcagatc tggaaggatt gttcgcccgg
     2041 ctagaggacg aattcgaaat caaggaacgc catttggcgc tcgaacgcaa gctgaacctg
     2101 atttccgata ccgcgcacac gctattggaa ctgatgagca gtgagcatgc cttgcgcctg
     2161 gagtggtaca tcgtgattct gatcgcggtg gaaattctgc ttactgtcta tcaactcttc
     2221 ctgagttaac cgtaacaccg taacggggca gaaggcgatg gttgggctgt tcaactcacg
     2281 ctggcgcaag atagcggctg tgcaaggttt gccggataaa cgcgcgcgcg tcttcacgta
     2341 caaaagactc gccctcggca agcttttcgg cgtaatcgaa gaaaggcgtg aacaactggc
     2401 cctggtctac cggcttgctc aatttgaagc ggcctgcgct agcgacttct cgccacgcgg
     2461 cacggacttc tgaccagaag ggctccgtct tttcaaaata atccttgccc gcgtcaaaat
     2521 catagttttt tattcgctcg tagcgcgcga cgccatattc ccgcgccaga aacggcagca
     2581 cggccctcgg cttgccgttc gcgttcagga ccaccttcag attattttct tcctgcaacc
     2641 atccggtctg cataatggtg tgccgattcg tcccgattaa agcctgatag tctttgcgta
     2701 cgctccattc gcgcctcggc agcggccgcc acgtttcatc gctgttccag gtggaaaagc
     2761 tgtcggtatg caaccagcgc cccgatgagg catagcgtgg ggaatcgtcc acttgataga
     2821 ccgattgcac ccatgcgccg cgacgctgat ccggcgggat cggtttgcgc atccatgtat
     2881 ctgctcccgt ataaaccagg gccgtttccg cctggtattg ccattcctgc cgccagtggc
     2941 gagtgaccag cggttcgctt ggcgtgccgt cctgctgaag tacgcgcatg accaaaatat
     3001 gttgaagcgc gatgaaatcg ccgctgtcct ggctgacgaa aacatattcg gttccccagc
     3061 tttggtaggg cgcatccggt ttcagtgacg gatcgaatcg aaccacttcc agaaaatcaa
     3121 agttgacgcg gtacgggccg gccatggcga ggatcgcgcg ccggtcacgc tcgagatcac
     3181 ttaggcctgc ctcgtgcagg cgcttccatg cttctgccgg ttcctggtcg agcgtgaccg
     3241 acacaccttt ggtgcttgca ccgcgtggtt caggtcctgc accgagcggc caggtgaatg
     3301 tataacgcgc gcttgccgcg gcttcacccg atcccgactc ataggcatgc acggccgtgc
     3361 acaccaggaa aagcgctatc acggttctag tcatacgcat ggcggctgct cttttctcaa
     3421 agcggacaat tacattttag aggaacgatt gcgcacaacg ttactggtga tcgctcgcat
     3481 gggatgccgg ttggcgcaat gacatttgca aactccgatg gctgtgtcgc ggtcaaaaac
     3541 ttgaccgcaa caggtgcgcg gactgacagg ctaactacta cgagagggaa aagatgatga
     3601 agttcaatgc gacgctgaaa gcactggtat tgattgtagc aattgcgggc ctgagtgcct
     3661 gccagaaagg tccggcagaa aaagccgggg aaaaaatcga taacgccgct aaagatgttg
     3721 gtaacaaaat ggaacacgcc ggcgacaaaa tcaaggacgc ggtgaagaag taagactgga
     3781 cgtcgcgacc tacgccgggc aagacgcgct catcgccgtc gagcggacaa taacttgtgc
     3841 acgctgatct tgcgtacctc ggttttgctg gtttcgatat gcttgcgcaa caggacaatc
     3901 gcccggtccg cacgccgcgt caataccgcg cgcagtatct gtgcatgctc ttcgtaggtt
     3961 gcggcgatgc ggcttgcttc cgtgaaatcc agccggcgca gtagcctgat gcgctcggtt
     4021 acttcctggt ggcatcgtgc catctcccgg ttgcgggcag ccgcgaccag cgcgccgtga
     4081 aacgcttcat cgagcgcgca gacctgctcg ccgtcgtcca gcctgcgttt cggttcaatc
     4141 agccagatgg atttcaaatc gtccagggcc ggtagttccg cgcgcgcaca cacggtttgc
     4201 acgcaagccg attcaagtgc gatgcgcaag tcatacaggt cgtcgaactg tgtgaaatcg
     4261 agctcccgaa cactccagcc gctgcggaat tgcacttgca gataaccttc tcgttcaagc
     4321 cgatacagcg cctcgcggac cggcgtgcgg ctgacgtgca ggcgcgcagc aatttcattt
     4381 tcggtaaaac ggtcgcccgg cagcaaacgg aaatcaaata tgttctgttt gacgccaagg
     4441 taaaccgaat cggccagaga agctttccgg gcatccacgg gtttcgaggc gctcgtccgc
     4501 cgcgaatggt ttttcggttt tgcgggtatg cgtgcgaccg tacgattcaa tgtgcagctt
     4561 tcctggccaa gaacgctcgc cacccgccca ggtgcgatat ttcttcggcg ccccgggtgg
     4621 cccaggcttc gcagatgaaa gcctgtaccc attcaccggt gtcgagctcg atctgtccga
     4681 tgcctagcgg cgacggaata ccggctacga atgacccgta ttcatgcatt ggcaaccgcc
     4741 agacttccac gtcaattgct cttcccaatc ctggatcgcg gaccatgcct ggtttcggcg
     4801 gcgtagtccc cggcagcgca tagagccggt agttggcgct ggtacgcgcg gctttttcca
     4861 accgcccgcc acgctcggtg agctgataat tgagcggcaa tccggacaaa tgcgcgccga
     4921 ccactgcaac ccgcacgtta tggtcggcta ttgtgatcgg ctcggccgcg ggaagtgtct
     4981 cgtccagcgc gcccagttgc aatccagtga gctgatgaaa ccgctgcgcc aaatcggcga
     5041 gcatcagatc gctgccggcc ggcgcgatga aggtaatgcc gctgggcagc ccgtccgggc
     5101 gcatcgatgc cggcacggca aacgctgcaa ggtcgagcaa attgacgaaa ttagtgtagt
     5161 aaccgaggcg gcgattggtc tggaacggct ctgccagcac ctccgcgacg gtgtacgcag
     5221 taggcgcagt gggtactacc atgacatcgg cacggttcca caattgtgct gtcgctttct
     5281 tcatcgctgc gatgcgggtc agcccggcaa acgcgtctgc ggcagaataa tctgacgcac
     5341 cctctatgat tttgcgtatc acggggtgca cctggtcagc atgctgctcg aaaaaagaac
     5401 ggatcgcagc gaggcgttcg gcgacccagg gtccttcgta cagcagcgcg gcggcgcgca
     5461 agtagggcga aaaatcagtg cggattggct tgccacccag gccctccagt ctgtccaccg
     5521 cttcattgaa tgcgtgcatc gcatgggcgt cgccatagaa ctccaggtgc tccggaagcg
     5581 cgaacgtgaa gcgctcgggc agtctcggcg cttccccggt cagctgtcgc gaccaaggat
     5641 cttctgcatc gtagccgcgc atgacccgca ggaccgcaac tgcgtcggcg acggtaagcg
     5701 cgaacaccga gacgcagtcg agcgactgac aggcaggcac tacgccacgc gcgctgacca
     5761 ggcctcggct cggcttcaag cccacgatgt tgttcagtcc ggcgggcacg cggccggagc
     5821 cggcagtatc ggtgccgagg gaaaaactca ctacgccgcg cgcaacggtc actgcggagc
     5881 cggaacttga gccgcccgat atgtattccg ggcgaaagct gttcggtaca ggtccatagg
     5941 gcgagcgcgt gccgaccagg ccggtcgcga actggtccag attagttttc ccgatcatta
     6001 tggcgccggc cgcctcgagc aattccactg ctctggccga gcgctcggga acgcgcgaaa
     6061 acgccgggca ggccgccgtg gtgggcagtc ccgccacatc gatgttgtcc ttgaccgaga
     6121 atggaatacc gtacagaggc atgcgggaca gcgcgttggc atccgcgctg gccaatgttt
     6181 cgagctgccg cgcgcgccgc accaacatgt cgtgctcaac ccggcatatc caagcgctgg
     6241 catagatgcc gtcccgagcc gaccccagtg attcgatgac ttcagaaggc gcgcgcttgc
     6301 cctggcggta atcggcaagc agcgatgata tggtcatgga cactgctttc ccccgtgcgg
     6361 catcttgtat accattgagt atacctacgg acggtgcgtg gaatctaccg ctgcacgaca
     6421 acacgcgggc ttgcaaggga gaagtaatgg acaaaatggc cattgaagcg gaaccgtatg
     6481 aactggaatt caatccgaag aacaccgcgt tgctgatcat cgacatgcaa cgcgatttcg
     6541 tgatgccagg cggattcggc gaggcgctgg gcaacgatgt tacgccgctg caagccacca
     6601 tcgcgccaac ggcacgcgta ctcagtgcgg cgcgtaaacg cggcatgctg gtcgtgcata
     6661 cccgtgaagg ccaccggccc gatctcactg attgcccgcc ttccaagctg gtgcgcgggc
     6721 gcggcaagac gcgcattggc gatcacgggc ccatgggtcg tattctggtg cgcggcgagg
     6781 tcggccacga catcgtgccg gaactgtatc cgcgcagtgg cgagccagtc atcgacaagc
     6841 ccggcaaggg cgcgttctat gccacggatc tggaactaat tctgcgagat cgcgctatca
     6901 agaccttgat tgtctgcggc gtgaccaccg aggtatgcgt caacaccact gtacgcgagg
     6961 cgaacgaccg cggttacgaa tgcgtggttc tgtccgattg tgtcggatcg tactttccgg
     7021 agttccagaa attcgcgctt gaaatgatca aggcccaggg cggtatattt ggctgggttt
     7081 cggactccac tcgtgcgctg gccgccatcg gcatctgacg cgagcggtcg gcgaaccgtg
     7141 cctgccgggc cgaccgatac gcaggcgtgg cgagctgaat ccattggcga cgccgtaccg
     7201 ttgcgggtga gcggaacggt gcatagcgtg tttgcgcacg cgtgcaacat cgagactgca
     7261 agcggcgact tggtgacact actggcgagt acccagggaa atggtccgcg cgggattaag
     7321 ctggctcatg cagcagcgct gttgtatgcg cggttgtctg ccggacagga agtacttatc
     7381 gggcaggcgc tactgcgggt gccggctgca gcagtcgcca ttgattttgc tggtgcccgc
     7441 gtctggtctg gtgaaatcgc cgccgtgagc ccgaatgttt gcgccagagc gctgcgccgg
     7501 gtgcgctcga tcctatgcga gcgcgttttc ttgcagggat ttgtgccgct gatcgccggg
     7561 tcacgccctg ctcagtctgc cttcgatcgc gcatttatcg aacgcctgtc gtccacgctg
     7621 ccagcgctcg cccaggccac tgtgcgccat gacagcgttg aggtagccgg cattgcggcg
     7681 caactggtgg ggctgggacc cggactcacg ccgtccggcg acgactttct caccggttac
     7741 cttgctgcct tgtggagccg cgccgggacc gatggcggca tcgcagctct gctaacaagg
     7801 atagatgtgt cactcacacc gctgttcctg cgaaccaatg ccattagccg gcagatgctg
     7861 cgcgatgcgg tgcagggacg attcgcggaa cacctgcttg acgtcatcag cgccttggcg
     7921 catgcgggtg atgtcgtcca cgcgaccatg cgagcgctgg agagcggcca cagttcaggt
     7981 gccgacacgt tatgcggcct gctgttcggt tatgcgccgc acatggcaat cccttcggtg
     8041 tctgggccgc ttatgatgcg aagcaagctt ggcgtgggcg cgctggcctg gatcaacgga
     8101 gaatcagcat gtcctctctc ctggtaaaaa tcgttccccg cgaataccgg gattcggttt
     8161 cgctgatgca actctccgcg atgctcggca aaatgccggg gatcgagcag gcttcggctg
     8221 tcatggctac agagaacaac ctcgcattgc tcgctgaagc cggattgcgc gttgacgcca
     8281 agggggcgaa cccgagcgat ctgctgattg tggtgcaagg tgacaaagcg gaactgcccg
     8341 cagcgattgc tgaggcgatg aaaaggctca cgcgccaggg cgaagacaat gccggcgaag
     8401 cgcgcgcagc attgcgcccg cgcagcatcc agatggcatt gaagctggcc ggtgaatcga
     8461 acatggcgct catttccaca ccgggagatt atgcgggagc agaggcgatg aaggccttgc
     8521 gcctgggcct caatgtcatg ctgttcagca ataacgtgtc cgtggaagag gaacttgccc
     8581 tgaaacagtt cgcgcgcgaa cgcgaattga ttgtcatggg gccggactgc ggcaccgcga
     8641 tcgtcaacgg gatttcgctt gctttcgcca acgtggtgag acgcggaccg gttggcgcga
     8701 tcggcgcttc cggcaccgga ttgcaacagg tcacggtgct ggtccaccgc ctcggctcgg
     8761 gcattacgca ggcgattggc acaggggggc gcgacttatc aaaagagatc ggtggcattt
     8821 cgatgctgaa agggctgaag gatcttgcgg ccgacgccgc cacgcaagtc atcgtgctga
     8881 tctcgaaacc gccggcgccg gaagttgccg aacgcgtgct cgcgcaagcg gccaaggccg
     8941 ggaagccagt agtggtggtc ttcctcggcg cggatgccaa aagtatcgcg cgcaagggcg
     9001 tgcacgccgt caacacactt gaggatgcag cgcgcgcagc cgtggcaatc gtcaagggag
     9061 acaagccgcg tgagagctat gccgatgtgc cggacctggc cgtcgggctg ccgaaattcg
     9121 cagaatcgca acgctacata cgcggactct acagcggcgg cactttctgc tacgaggcca
     9181 cgctgttgct aggcgcactg ctgcccgagg tgtactccaa caccgcgaca cgcaatgcgc
     9241 atgagctggc ggacgtctgg cagagccatg ctcacacgtt agtcgatctc ggggatgatg
     9301 tcttcacgcg cggccggccg catccgatga tcgattaccg gctgcgcacc gaacgcatcg
     9361 tcaaggaagc ggaagatccg gaaacagcgg tcattctgct cgatgtcgtg ctgggctatg
     9421 gcagccacat ggatccgtcg agtgagctgg tgccagccat ccaacgcgcg cgtgcaatcg
     9481 cggcgacgca aggtcgcgcc gtgagctttg tgggacacat ttgcggaacc gcgtccgatc
     9541 cgcaggatct tggccgccag acccgggcac tcagcgaagt cggcatgttg ctcgcagaga
     9601 gcaatgcgca ggcggtgcgg ctcgccgcgg cgattgccgg taaaaatggt cctttgcaac
     9661 gcgcgaatac tgcagaaaaa gaaacgaggc ttcgatgagc gcgctgtttg gggaaggcca
     9721 gagcattgtc aatgtcgggc tgcagtcctt cgccgactct attgtggcgg gcggtggcaa
     9781 ggcggtgcag gttgattgga caccgccggc gggcggcaat cgtgtcgcag gctggagcct
     9841 ggcgcaactg ctgaaccatc cagacgtgga gcgcgccaat gaaacggcct tcgcggctta
     9901 cctggcggcg caaccggtgc tggtcggcat cggcgttgcg ggcgagcaat tgccggacat
     9961 gaaagcacgc atgattctgc acgccggtcc gcccatcgat tggcaggaca tgtgcgggcc
    10021 gatgcagggc gcggtgatcg gagcgatcct gtatgaaagc tgggccaatg atgcggatgg
    10081 cgcgcgccgg ctcgcggaag acggcgccat cgcattcgca cctgcgcacc atcacgccgc
    10141 tgtcgggccg atggcgggtg tgatcagtcc ttcgatgccg gtatgggtaa tcgagaacgc
    10201 ggccggcagc aatcgcgcct attccaacct gaacgaaggg ctgggcaagg tgctgcgttt
    10261 cggcgcgaat tcgggtgaag tcatcaagcg cctgcgctgg atggccgagg tgcttgcgcc
    10321 cgcactcagg gcggctctga cgacattcgg gccgctggag ttgaagccgc tcatcgcgca
    10381 ggcgctgcag atgggcgatg aagtgcacaa ccgtaatgcg gcggcatcct cgctgtttct
    10441 gaaaaggatc attccggccg cgttgcattc gggattcgat gcaggcgcca tcgccgaggc
    10501 agtcacattc attgcaggca acgatcattt ttttctgaac ctgtcgatgg cggcttgcaa
    10561 ggtgatgctc gatgcggcgc acggcgtgaa gggaagcagc ctcgtcaccg ccatgtcgcg
    10621 caatggcgtg cgatttggtg tgagactctc gggcaccggc gcgcagtggt tcgaagcgcc
    10681 ttctccgatg gtaggcggcc tgttctttcc aagctatggg cccgagcacg cagcccccga
    10741 catgggagac agctccatca ccgagacggc ggggcttggt gggttcgcca tggccaccgc
    10801 gccggccatc gtgcagttcg tcgggggcac gccgcaagat gcaatcgcca acacgcgtga
    10861 aatgcattac atcacgctgg gacgcaacag cgcatttacg ctgcccgcca tgaatttcgg
    10921 cggcacacct gccggcatcg atgcacggcg cgtgctggat accaatatca tgcctatcat
    10981 caataccggt atcgcacaca aggaagccgg catcgggcag atcggcgcgg gcattacccg
    11041 tgcaccactg gaatgcttta cgcaggctgt cgttgcactc gcatcacggc tggacgttgc
    11101 tgtgcctggc agcattcccg ccagttagcc tagcgacacg caggagaagc ccggatgacc
    11161 aaacgcgaac acacggcagt cgtggcggtg ggtggcaatg cgctcatcat cgatgagcat
    11221 caccaatcga ttcccgatca ggtgaaagcc gcaacgctga cttcgcatta cgtcgccgac
    11281 atggtggagg cgggttggac tgtggtgctg acgcacggca atggccccca ggtcggtttc
    11341 atcctgcgtc gttccggcat tgccaagggt gaggtcccgg aggtgccggt cgatttcgcg
    11401 gacgcggata cccagggggc catcggctac atgttccagc gcgcgctcta caatgaattc
    11461 cagcgccgca aactcaagcg caaggtggtc gccatcgtta cccaagtgcg cgtcgatccg
    11521 cgcgatccgg cgttcaatgc gccgaccaaa ccaatcggtg cgcacatgac cgaggacatc
    11581 gccaaggcgc tggcgcagga tctggggtgg acagtcagag aagattccgg gcgcggctgg
    11641 cgcagggtgg ttgcctcgcc acaacctcag gaaattatcg agttgggaat tatcgaggcg
    11701 ctgatcgatt tgggctacat cgtcattgcc tgcggcggcg gcggcattcc ggtgctgcgc
    11761 gacgggaaag gcgagctgca gggactcgag gccgtgatcg acaaggattt tgcgtccagt
    11821 ctgctggcgc gcgacctgcg cgcggaactg ttcgtagtct ccaccggcgt ggaaaaagtg
    11881 gcagtgaact tcggcaagcc cgagcagcgc tggctggaac gcgtgggact cgatgaggcg
    11941 cgccgacacc ttgccgatgg tcaatttccg aaaggcagca tgggccctaa gatagaggcg
    12001 gtgattgcat tcctcgaagg cggcggcagc cgcgcactga ttaccgaccc gcccaatctg
    12061 gggcgcgcac tctcgggcgc gaccggcacc catatcgtgc cggcctgaga gcgcgcgctc
    12121 ttgctgaaga gtccttaagc gcgaacaggt ggcgcatcct ggcgtgagag caccaggcac
    12181 gctgcaccga acatcacata tcccagcgcc accagcggcg acaccatcca ccccagttgt
    12241 ggtccatgga tcagaccgat gaatgagaat gccgcgccca caaatgcaac gacggcggcc
    12301 tgtgccagct tgcggtcgat gatgaaggct gccatggcac cgagcaccat gccggcaagc
    12361 accgagccgc cgccgagtac cgtgagcccc tgatacacca cacccacctg cccaagcgcg
    12421 gccatgccga ctttgtcagc gctggtgccg gcggcaccca acgccccgtc gatgttgatt
    12481 ttcgcccact cggcaatgtt tggaatgatc gcaagcacga ctgccggcgc atggttggaa
    12541 gggcttgcct ggaatgcctg cgccccgatg accagaccaa tgtagagcag gatcggcagg
    12601 attgcgacca gcggaatcag attcagcaat agtgcggtca gtcccaggaa gcagaccagc
    12661 gcaatggcaa tgccggtagc gaaggagtag ccgatgcgcc cgcctaccga tttccacccg
    12721 ggatggccaa tgtacacggc cggcgggaat gggcttccga gcatggagcc gacaattgcg
    12781 ccgataccgt ccgccgcaag aatcttgcgc aaattgtagt tgtccccggc ggccgacgcg
    12841 ctttccacat tgttcatcgc ttcggtgaag ttatagatgc cgagcggaat cgctgttgcg
    12901 agcagtggcg caatgccggc cagtccgcgg aatacgtcgc cggaaaacca tggcagatgc
    12961 agtccgaaat gctggaaggc cttgctgacc tcggcaccct tcatgtagtc gctccagcca
    13021 aacgccgcgg caatccagcc gatcagcgcg cccagtacaa ctgcagcaag gccgccggga
    13081 atgcccccag gcaggcgaat gttcgcagtc cacgcgatca gcacaattgc gaagcatacg
    13141 aagccgatcc acggcacctc ccacatctgg aacgccggac gcatggaaat gaatgcgatc
    13201 gagatgccgg caagcgtgcc caacatcgcg gcgcgaggcg tcattttgcg aatccagggc
    13261 ccgacgaagc agccgagcag cacaatgatg ccgatgatga agcaccaggc cagtccagca
    13321 gcgtaagcca gcatcgggtc cttggtggac aggtaagtcg gcagcatcac caccagaacg
    13381 acgatgaaca tgtgcggcac gctgggtccg tatggcatgg ccgtgacgtc cgagcggcca
    13441 gtctccttcg ccagtctgta tgcgagccag gcatagaaca ggttgccaat aggcagcgca
    13501 atgccaagcg caggcagcac ccgcccgtac accgtgtctg gcggcaactg caccacatac
    13561 agacataacg tcgtcaggac gatgacgttc agcagcacgt tcgtgaacaa accgaagaaa
    13621 ccgttccagt cgccttgcac ccaccaactc gggtttccag cagatgcggc tgtcattcga
    13681 gagcttgcag cagtttcggc agtactcatg aattttcctc tcctgtgtga ttatttttat
    13741 aatgttttag gtcagatgaa ccggcgcagg cagcgtcctt actgtacgga catctccctc
    13801 ccacgcctcc aaacacacac tggccgcgga agcatgcgcc agcgcggtcg ccaagtccag
    13861 aacacgggcg gtctggccct gtgcttacgg cggcgcgctg aagacaacct gccaatatgc
    13921 ccgtcggtgg ccaggggagc acgttagtgg cagcgtgacg cccgatcgca atatggcctt
    13981 gagccgccaa attttgatcg gtgcgaacac gcctttttgg ggccccgaaa catcctgcgc
    14041 gtggctgcaa tagtggcgct aatgaccaag gccgttgaac caggggcgcg gcaaagcagc
    14101 tccggcggcc gtgaaatgat ccgcgcctca ggcgctcctg aagaaaaaat caggggttcg
    14161 ccgtaccggc ttagggcaaa tgactagcgg atgcattctt cggtgtcatg ttactacgtg
    14221 atgatgttaa gcggtccaac tcgccgtcag gaaactacat gaaatccttc gaacgccatc
    14281 gcatacggtt aagacaagtt ctgataatgc tactgggggc gttagccact gggtgcgggc
    14341 agggtagtag caacagcggg ccagctgtgg cccatgtggc ggtgacctgc ccagtggaac
    14401 gaattaatgc actggaaaag gcgatggaca acacgctttc ccaggcgaat accgaagtcg
    14461 atttctcttt tgccgtggaa aaaaaggatg gccgccgcta cgtcttcaac cgcggagcat
    14521 ccaccatgca aaccgcctat caaaccgcat ccacgtcaaa aatg
//
LOCUS       DATMZQ010000217         7809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4227411, whole genome shotgun sequence.
ACCESSION   DATMZQ010000217 DATMZQ010000000
VERSION     DATMZQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4227411"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VN496_07035"
     CDS             complement(<1..170)
                     /locus_tag="VN496_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXF16741.1"
                     /translation="MQVFRGIPARADVPIALTIGNFDGVHLGHRAMIARLSAAGRRKN
                     LPVALMTFEPQP"
     gene            complement(253..645)
                     /locus_tag="VN496_07040"
     CDS             complement(253..645)
                     /locus_tag="VN496_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HXF16742.1"
                     /translation="MSQTRGSAIVLFAHGARDPSWAEPFHRMVERLRQKQPATRIELA
                     FLECMDPTLESAVQALATEGVARITVVPLFLAQGGHLKNDLPKLLADIRGEHPALQIE
                     VTASIGDSEDLTNAIADWAFAQHVSRVG"
     gene            complement(674..1744)
                     /locus_tag="VN496_07045"
     CDS             complement(674..1744)
                     /locus_tag="VN496_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HXF16743.1"
                     /translation="MTIEIRNLSKRFGTFSALRDVSLQVQHGELLALLGPSGSGKTTL
                     LRVIAGLEAPDTGQVLFQGEDSTDRDVRDRRVGFVFQHYALFRHMTIFENVAFGLRVR
                     QKNVRPAEESIQERVFKLLKLVQLDWLADRYPHQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRSWLRRLHDDMHITTVFVTHDQEEALEVADRVVVMNEGRIEQI
                     GTPDEVYDHPAGRFVYEFLGNVNLFHGRLHRGRALIGDIELDAPGHADAEQQPAVAYV
                     RPHDIEIERVANKGTFAVRILHVLAVGPVVRVELVREDHGQEQPIQAEISRERFRQLQ
                     LVAGERVFIRPRRFDLFPSPLH"
     gene            complement(1741..2613)
                     /gene="cysW"
                     /locus_tag="VN496_07050"
     CDS             complement(1741..2613)
                     /gene="cysW"
                     /locus_tag="VN496_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HXF16744.1"
                     /translation="MSELALPPVPLRSGLHAAQEPAWLRWTLIGCALAFLTLFLFVPL
                     ISVFYEALKKGAGVYFVSIVEPDALSAIKLTLIAAAIAVPLNVVFGVAAAWAIAKFEF
                     HGKNLLITLIDLPFSVSPVVSGLIYVLIFGLQGWLGPWLAEHDVKIIFAVPGIVLATI
                     FVTFPFVARELIPLMQAQGSEEEEAAALLGANGWQIFYRVTMPNIKWGLLYGAILCNA
                     RAMGEFGAVSVVSGHVRGMTNTMPLHVEVLYNEYHFAAAFAVASLLALLAVVTLALKS
                     VVDARVTTERGTKP"
     gene            complement(2624..3463)
                     /gene="cysT"
                     /locus_tag="VN496_07055"
     CDS             complement(2624..3463)
                     /gene="cysT"
                     /locus_tag="VN496_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HXF16745.1"
                     /translation="MRQNAIKRHSVLPGFGLALGFTVVYLSLIVLIPLSAAFIKTTAL
                     SWPEFWATVTTPRVMASYRLTFGASLAAALLNAIFGLLVAWVLVRYRFPGKQIVDALV
                     DLPFALPTAVAGIALTALYAGNGWIGRWLEPFGIKVAFTPIGVFVALTFIGLPFVVRT
                     VQPVLEDVEPELEEAAATLGASRLQTFARVIFPMVLPALVTGFALAFARAIGEYGSVI
                     FIAGNMPMISEITPLLIITKLEQYDYAGATALAVVMLAISFVLLLIINGLQWWSRRRG
                     ESS"
     gene            complement(3480..4490)
                     /locus_tag="VN496_07060"
     CDS             complement(3480..4490)
                     /locus_tag="VN496_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXF16746.1"
                     /translation="MPSTSQIAIRAAAVLLAASAWAASAANVQLLNVSYDPTRELYQD
                     FNAAFAKQWAAKTGDKVTIKQSHGGSGKQARTVIDGLDADVVTLALAYDIDEIAARAK
                     LLPKDWQKRLPHNSSPYTSTVVFLVRKGNPKRIRDWDDIARPGISVVTPNPKTSGGAR
                     WNYLAAWGYALKSSGNDEAKAKDFVSKIYKNVPVLDSGARGATTTFVERGIGDVLLAW
                     ENEAFLAVKELGHDKVEIVVPSVSILAEPPVSLVDRFADKHGTRNVAQAYLEYLYSEE
                     GQDIAGRNFYRPTDPKVAAKYAGQFPKLTLFTINDMFGGWQKAQKTHFADGGVFDHIY
                     QK"
     gene            complement(4502..5878)
                     /locus_tag="VN496_07065"
     CDS             complement(4502..5878)
                     /locus_tag="VN496_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXF16747.1"
                     /translation="MHPIRTRILVGALAASAVLVSAIGKADDDLSQLKEQLHELDQKI
                     RILQRQQELDKEAAAEKVKETPTVSTGKDGFWLRSADNDYQIRIGGVIQADARVYPSK
                     TTPTSATDSFVMRKVRPVLEGVFLQNIGFRLMPDFGNGTTVLQDAYVELMLDKAFNIR
                     AGKFKAPFGLERLQQDVDTEFVERGLPTNLSPNRDTGIQLGGSVLASTLYYQVGVLNG
                     VADNSTTDGDTNDEKDLVARIFAEPFRNGDSWLSGLGIGIAYAHGVQTGTATSSNLAT
                     YKTVGQENFFVFNTGAFADGERTKLAPQFYYYNGPFGLLGEYVDSRQVVTRATNQKRD
                     FDSKAWQLNGFWVLTGEDASFRNPTPRRPYAGSNPGMGALELVTRYGVQTINESAFVG
                     TAATRLANPNVSVREARGAGIGINWYFNRNFKVQLNYDQTEFDGGGPGGTDMQTEKTV
                     FTRVQANF"
     gene            complement(5949..6227)
                     /locus_tag="VN496_07070"
     CDS             complement(5949..6227)
                     /locus_tag="VN496_07070"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HXF16748.1"
                     /translation="MTRSEESQAEDAAPRDRLPAEVEEQIFRAISRVHYGSIEIMIHD
                     GCVVQIECREKIRLGRDQPGTKSAAREIRTSTTARRSATEATRRLEPA"
     gene            complement(6247..7242)
                     /locus_tag="VN496_07075"
     CDS             complement(6247..7242)
                     /locus_tag="VN496_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXF16749.1"
                     /translation="MVRTLIALLLLLGEIQGALAATTLLNASYDVTREFYRDFNSAFV
                     SYWKARTGETVTVNQSHGGSSKQARAVLDGLDADVITMNQANDIDFLAERGRRLVVAD
                     WSKRLPYHSAPTTSTTLFLVRKGNPKHIRDWDDLVKPGVSVVIPNPKTSGNGRYSYLA
                     AWGYVVRNGGSEQQAREFVTRLFRNVPVLDTGSRGATTTFVQRGIGDALLTFENEVIL
                     ARNELGDDAFDVVYPSVSIEAENPVSVVDKVVDRHGTRKIATAYLEYLYSPEGQEIAA
                     RHFLRPRDAAVLARHESRFGKLQLFTVEDVFGGWKKAQAVHFSDGGLFDQIYQTK"
     gene            complement(7463..>7809)
                     /locus_tag="VN496_07080"
     CDS             complement(7463..>7809)
                     /locus_tag="VN496_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16750.1"
                     /translation="FVTPRVGEGEDESDWAQVVAAADTAVLYMAAGQAGAISRILRQH
                     GMPASTPAVIVVNVSLAAAQRIHTVLERLEHDVPTQLAGPAVLMIGSVFASDRAIRHA
                     VDEALSLTANSR"
BASE COUNT         1604 a         2337 c         2232 g         1636 t
ORIGIN      
        1 tgaggctggg gctcgaaggt catcagcgcc accggcaggt tcttccggcg gcccgcggcg
       61 gatagtcgcg cgatcatggc gcgatgcccg aggtgcacgc catcgaaatt tccgatggtt
      121 agcgcgatag gaacatctgc ccgcgccggg atgccgcgaa aaacttgcat gtccaagcga
      181 ccaaaagccc gaattttagc ggctttcggc tacacggagc gcggttttga acgactgaca
      241 tcggaatacc ccttagccca ctcgggacac atgctgcgcg aaggcccaat cggctatcgc
      301 gtttgtgagg tcttccgaat cgccgattga ggcagtaact tcgatttgca gcgcggggtg
      361 ctccccgcga atgtcggcga ggagcttggg taaatcattt ttgaggtgac cgccctgggc
      421 aaggaacagg gggacgacag tgatacgggc cacgccctcg gtggccaacg cttgcacggc
      481 cgattccagc gtgggatcca tgcattccag gaacgcgagt tcgatacgag tagcgggttg
      541 cttctggcgc aggcgttcga ccatcctgtg aaacggctcc gcccacgaag gatcgcgcgc
      601 gccatgcgca aacagcacga tggcggagcc tctcgtctgg ctcacgttga actcaaccgc
      661 agtcgcatcg cgcctagtgt agcggactcg ggaaaagatc gaagcgccgc ggccggatga
      721 atacacgctc cccggcaaca agctgcaatt ggcgaaaccg ctccctgctg atttcagcct
      781 ggatgggctg ctcctgccca tggtcttcgc ggaccagttc tacacgcact acaggaccca
      841 ccgccaacac gtgcaggatg cgaactgcaa atgtgccctt gttcgccacg cgctcgatct
      901 cgatatcatg cggccggaca taggctacag cgggctgttg ctccgcatcc gcgtgcccag
      961 gcgcatccag ttcaatgtct ccgatcagtg cgcggccccg gtgaaggcgt ccatgaaaca
     1021 aatttacatt tccaaggaat tcgtacacga accgcccggc cggatggtca taaacttcat
     1081 cgggcgtgcc tatttgctct attcggcctt cgttcatgac caccacgcga tccgccacct
     1141 ccagtgcctc ctcttgatcg tgggtaacaa aaacagtggt gatgtgcatg tcatcgtgaa
     1201 gccgccgtag ccagctgcgt aattccttgc gcaccttggc gtccagcgca ccgaacggtt
     1261 cgtcaagcag caataccttt ggttcgaccg ccaaagctcg cgctagagcg atacgctggc
     1321 gctgtcctcc cgagagttga tgcggatagc ggtcggcgag ccagtcgagc tgcacaagtt
     1381 tgagtagctt gaaaacacgt tcctgaattg attcttcggc gggtcgcaca ttcttctgcc
     1441 gcacacgcaa gccgaacgcg acgttctcaa aaatggtcat gtgccgaaac agcgcgtaat
     1501 gctggaagac aaatccgacc ctgcggtcgc gcacatcccg atcagtggaa tcctctcctt
     1561 gaaacaacac ctgaccagtg tcgggagctt ccagtccggc gatgacccgc aacagggttg
     1621 ttttaccgga cccggaaggt cccagcaacg ccaataattc gccgtgctga acttgcaagc
     1681 tgacgtcgcg tagcgctgag aacgtgccga atcgcttcga gagattgcga atttcgatgg
     1741 tcatggtttt gtcccacgtt cggtggtgac ccgcgcgtcg acgacgctct tgagtgccag
     1801 cgttaccacg gccagcaagg ccagtagcga ggccactgcg aatgcagcgg caaagtggta
     1861 ttcattgtaa agaacttcga cgtgcagcgg catggtgttg gtcatgccac ggacatgtcc
     1921 tgacacgaca gacactgcac caaactcccc catcgcacgt gcgttgcaca gtatcgcccc
     1981 gtacaacaat ccccatttga tgttcggcat ggtgacgcgg tagaaaatct gccagccgtt
     2041 ggcaccaagc agcgcggccg cctcctcttc ctcgctaccc tgtgcctgca tcagcgggat
     2101 cagttcgcgg gccacaaaag gaaaagtcac gaaaatcgtg gcgagaacga ttcccgggac
     2161 cgcgaaaatg attttgacat cgtgctccgc aagccaggga ccaagccagc cctgcaggcc
     2221 gaagatcaat acatagatga ggcccgacac caccggcgac accgaaaacg gcagatcgat
     2281 gagcgtgatc aaaaggttct tgccgtggaa ctcgaacttt gcgatggccc atgcggcagc
     2341 aactccgaac acgacgttga ggggtactgc aattgccgcg gcaatcaatg tgagcttgat
     2401 agcggacagt gcgtcgggct cgacaatgga gacgaaataa actcccgcgc cttttttcaa
     2461 tgcttcgtag aacactgaga taagcggcac gaacagaaac aacgtcaaaa acgccagagc
     2521 acacccgatc agtgtccagc gcagccatgc cggctcctgg gcggcatgca agccgcttcg
     2581 caatggcacg ggaggaaggg ctagctcact catggacagg tcgtcacgat gattccccac
     2641 gccgccggct ccaccattgc agcccgttta tgatcaatag caggacgaag gaaatggcga
     2701 gcatcaccac ggccagcgca gtcgcgccgg cataatcata ttgctccagc ttcgtaatga
     2761 tcagcagtgg cgtgatttcc gaaatcatcg gcatgttgcc ggcaatgaaa atgaccgatc
     2821 cgtattcgcc gattgcgcgc gcaaacgcga gtgcaaagcc ggtaaccagc gctggcaaaa
     2881 ccatgggaaa tattacgcgc gcgaaggttt gcagccgcga agcccccaac gtggcagccg
     2941 cttcctcgag ttccggctcg acatcttcga gcacaggctg cacggtacgc actacgaacg
     3001 gcaggccaat gaatgtcagc gcgacaaaga ccccgatggg cgtgaacgca accttgatgc
     3061 cgaagggctc caaccatcgc ccgatccagc cattgcctgc atagagtgcg gttagcgcaa
     3121 ttccggcaac cgccgtcggc agtgcgaatg gcaagtcaac cagcgcatcg acgatttgct
     3181 tgccgggaaa gcgatagcgc accagcaccc aggccaccag caagccgaag atcgcattca
     3241 gcagcgctgc cgccagagat gcgccaaagg ttaggcgata cgaagccatc actctcggcg
     3301 tcgtcactgt cgcccagaac tctggccatg agagcgccgt cgttttaatg aatgccgcgg
     3361 acaatggaat cagaacgatc aggctcagat aaaccacggt gaatccaagc gcaaggccaa
     3421 accccggcag gacgctgtgc cgcttgattg cgttttgcct catatcgatc tcagtaccgt
     3481 tacttctgat aaatgtggtc gaacacgccg ccgtcggcga aatgcgtctt ctgcgctttc
     3541 tgccagccgc caaacatgtc gttgatggtg aacaaagtca gcttcggaaa ctggcccgca
     3601 tatttcgccg cgaccttcgg atctgtcggc cgatagaaat tccggccagc gatgtcctgc
     3661 ccttcctcgc tataaaggta ttcgaggtag gcctgcgcga cattgcgcgt gccatgcttg
     3721 tcggcaaatc tgtccacgag ggataccgga ggttcggcga gaatgctgac cgatggcacg
     3781 acgatttcga ccttgtcgtg cccgagctcc ttgactgcca ggaaggcctc gttttcccac
     3841 gcgagcagca cgtcgccgat gccgcgttcg acgaatgtcg tggtcgcgcc ccgggcgccg
     3901 gaatcaagga cgggcacatt cttgtagatc ttgctcacga aatccttcgc cttggcttcg
     3961 tcgttgccgg aacttttcag cgcgtagccc caggcggcca gataattcca gcgcgccccg
     4021 cccgaagtct tcggattggg cgtaacgacc gaaatgcctg gccgtgcaat atcgtcccag
     4081 tccctgattc gtttgggatt gcccttgcgg accaggaaga cgacggtgga cgtatacggc
     4141 gagctgttgt gcggcaggcg cttttgccag tccttcggca gcagttttgc cctggccgct
     4201 atttcgtcga tgtcatacgc gagcgcaagc gtgacgacat ctgcgtcgag tccgtcgatc
     4261 accgtccgcg cctgtttgcc tgaaccgccg tgcgattgct ttatggtgac tttgtcgccg
     4321 gttttagcag cccattgttt cgcgaacgcg gcattaaagt cctggtagag ttcgcgcgtc
     4381 ggatcgtagg acacgttgag cagttgcaca ttcgcagcgg atgccgccca tgcggaagcc
     4441 gctagcagaa cggctgccgc gcgtattgca atttgtgatg tggaaggcat tgtcgctctt
     4501 tctagaaatt cgcctggacg cgggtgaata cggtcttctc ggtctgcata tcggtgccgc
     4561 ccggcccgcc gccgtcgaat tccgtttggt cgtaattcaa ctggactttg aaattgcgat
     4621 tgaaatacca gttgatgccg atgccggcac cacgcgcttc tcgtacggac acgttgggat
     4681 tagcgaggcg cgttgcagcg gtgccgacga aggcggattc attgatggtc tgtacaccgt
     4741 atcgcgtaac caattccaat gcccccattc cggggttgct gcccgcataa ggtctgcgcg
     4801 gagtggggtt gcggaacgat gcgtcctcgc cggtcaagac ccagaaccca ttcagctgcc
     4861 atgccttgct gtcgaagtcg cgcttctggt tcgtcgcgcg cgttaccacc tgccgggagt
     4921 cgacgtattc cccgagcagg ccgaacggtc cgttgtagta atagaactgc ggcgcgagct
     4981 tggtgcgctc gccatccgca aatgcgccgg tattgaaaac aaaaaaattc tcctgcccga
     5041 ccgtcttgta ggtcgcgaga tttgaagacg tcgccgtgcc ggtttgcacg ccatgggcgt
     5101 aggctatgcc tatcccaagg ccgctcagcc agctgtcacc gttgcgaaac ggctctgcaa
     5161 atatgcgcgc caccagatcc ttttcgtcat tcgtatcgcc gtccgtggtg ctgttgtccg
     5221 caaccccgtt caatacgccg acctggtagt agagcgtgct ggccagcacg ctgcctccca
     5281 gctggatgcc ggtatcgcga ttgggagaca ggttagtcgg caggccgcgc tcgacgaact
     5341 cggtatctac atcctgctgc agccgctcca ggccgaacgg ggccttgaat tttccggcgc
     5401 ggatattgaa cgccttatcg agcatgagtt cgacgtacgc gtcctgcagt accgtggtgc
     5461 cattgccgaa atcgggcatt aaccggaacc cgatgttctg gagaaacacg ccctccagta
     5521 ccgggcgtac tttgcgcatg acgaacgaat cggttgcgct ggtgggcgtc gtcttgcttg
     5581 gatacacacg ggcatcagcc tgaatgacgc cgccgatgcg gatctggtag tcgttgtccg
     5641 cagacctcaa ccagaatccg tccttgccgg tggaaacggt cggcgtttct ttcacctttt
     5701 cggcagccgc ttccttgtcc agttcctgct gtctttgcag aatgcggatt ttctggtcga
     5761 gttcatgcaa ctgttccttc agttgcgaaa ggtcgtcatc ggctttgcca attgctgaaa
     5821 ccaggacggc gctagcagcg agcgcgccga cgaggatccg ggtccggatc ggatgcatgc
     5881 gaaaaaactc ccttgagtca tggcggagcc ttccggtgat ccggtcaggt cgtctcgttg
     5941 tcggttattc atgctggctc cagtcgcctg gtcgcctctg ttgcggaacg ccgcgcagta
     6001 gtggacgtgc gaatctcccg cgcggcgctt tttgtgccgg gctggtcgcg gcccaggcgt
     6061 atcttttccc tgcactcgat ctgcacgaca cacccatcgt ggatcatgat ttcgatggaa
     6121 ccgtaatgaa cacgggatat cgcgcggaag atttgttcct cgacttccgc gggcagccgg
     6181 tcccttggcg ccgcatcttc tgcttgactc tcttcgcttc tggtcatgat ttctgaaatg
     6241 cccccgctat ttcgtctggt agatctgatc gaacagcccg ccatcgctaa aatggacggc
     6301 ctgcgctttt ttccagccgc cgaacacgtc ctcgacagta aataactgca gtttcccgaa
     6361 tctcgattca tgccgcgcaa gtaccgcggc gtcgcgcggt cgcagaaaat gcctcgccgc
     6421 tatttcctgg ccttccggcg agtagagata ttccaggtac gccgtcgcga tcttgcgcgt
     6481 accgtgcctg tccacgacct tgtcgaccac gctgactgga ttctcggcct cgatgctgac
     6541 agacggatag acgacgtcaa aggcatcgtc ccccagttcg ttgcgcgcga ggatcacctc
     6601 gttttcgaac gtcagcaacg cgtcacctat gcctcgctga acgaacgtcg tggtggcgcc
     6661 gcggctgccg gtgtcgagta ccggaacatt cctgaacagt cgggtgacga actcgcgcgc
     6721 ctgctgctcg ctgccgccgt tgcggacgac gtagccccac gccgcgagat agctgtagcg
     6781 gccgttgcct gacgtcttgg gattgggtat cactaccgag acgcccggct tcaccagatc
     6841 gtcccagtcc cgaatatgtt tcggattgcc ctttcggaca agaaataggg tcgtcgatgt
     6901 ggtgggcgcg ctgtggtacg gaaggcgctt cgaccagtcg gcgacgacca gccgccgccc
     6961 gcgctcggcg agaaagtcta tgtcattcgc ctggttcatc gtgatcacgt cggcatcgag
     7021 cccgtccagg accgcgcgtg cctgtttgct ggatccgcca tgcgactggt tgaccgtcac
     7081 ggtctcgcct gtcctggctt tccagtaact tacaaacgca gaattgaagt ctctgtagaa
     7141 ctctcgcgta acgtcatagg aggcgttcag taacgtcgtc gctgccaatg caccctggat
     7201 ttcaccaagc aggagcagta aggcgatcag cgttcgaacc atttttttta ttcatccctg
     7261 tcatggcgcc cgctccgcga aaaaacggcg ccgggcgaac cggcgcgaaa acaacgcaag
     7321 gaggaggaca acggcatgca aggtacctgc tgttctttat gatttgaaat actataagtt
     7381 cattttatta ttgctaaatg tgatatagat aggaatgaca aaaaccggtg ttcggccggc
     7441 tgtcctgggc gggatttcct ggctagcggg aattggctgt tagtgacagc gcttcgtcga
     7501 cggcgtgccg gatcgcacga tcggacgcga acacgcttcc gatcatcagc acggcagggc
     7561 cggccagttg cgtcggaaca tcatgttcga gccgttccaa gacggtatgg atccgctgcg
     7621 ccgcagccaa ggaaacattt acgacaatga cagccggcgt gcttgcgggc atgccgtgct
     7681 ggcgcagaat gcgcgaaatg gcacctgcct gccctgcagc catgtagagc accgcggtgt
     7741 ccgccgccgc aaccacctgt gcccaatcgc tttcgtcctc gccttcgccg accctggggg
     7801 tgacgaaca
//
LOCUS       DATMZQ010000218         5379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4227951, whole genome shotgun sequence.
ACCESSION   DATMZQ010000218 DATMZQ010000000
VERSION     DATMZQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4227951"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VN496_07085"
     CDS             <1..770
                     /locus_tag="VN496_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXF16751.1"
                     /translation="GLSASLAAKEKHLRFITLEQEDSLGGTVYHYPRNKITMTSGMHV
                     PLIGRIKLGEVRKEALLEFWLGVISKVGLKVNFREQVNAIEKVSGGFKVTTSKHSYVA
                     RNVVLAIGRRGTTRRLDVPGEHQAKVVYRLIDPAQYRGQHVLIVGGGDSAIEAAIAIS
                     REPNTTVSLSYRGATFNRIKSANQVRLREVQKEGRLRVLLESETVEIQLRKIVLKHKN
                     NVFSLRNDVLIVCAGGLLPTQFLRNLGILVETHHGLA"
     gene            882..1682
                     /locus_tag="VN496_07090"
     CDS             882..1682
                     /locus_tag="VN496_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXF16752.1"
                     /translation="MTEQIDVNEGRITTELRDHLFLIGIDRPAKLNGFTPVMLRGMAQ
                     AYTRYEHEPLARCAVVFAEGAHFTAGLDLPKVAAHLGGNTLLVEKDEIDPFESRPPFR
                     SKPVVFAVQGICYTLGIEMMLAADIAVAASNTRFAQVEVKRGLMPTGGATIRMVQRAG
                     WGNAMRYLLTGDEFDAQTALTLGYIQEIVEPGRQRERAMELATSIADQAPLAVAATLS
                     NARVALREGPETAASLFGATQTRLLASEDFQDGLRSFKEKRAPNFRGK"
     gene            1745..2587
                     /locus_tag="VN496_07095"
     CDS             1745..2587
                     /locus_tag="VN496_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF16753.1"
                     /translation="MSGFVITAVLFGALLHAAWNVLVKSGGDKWLDVALLACASAVVS
                     SALLPFLPSPAPESWPYIIASAVVEVGYFALVAAAYRTGELSYVYPIMRGSAPLLTAL
                     AAGLFVTDRLTAGNWFGILLISAGLLLLPADQWWRGRFHVASTLFAFSTAVVICIYTL
                     IDGIGSRLSGNAFTYVACMMSLNGFLLAVFLFLQQPAAAMQRIRASWLPLLGGGLCAW
                     ASYAIALWAMTRAPIAMVAALRETSVIFGSVLAAVVLKEKFSMARYAAALLVCAGAIS
                     LKAL"
     gene            complement(2634..3887)
                     /gene="egtB"
                     /locus_tag="VN496_07100"
     CDS             complement(2634..3887)
                     /gene="egtB"
                     /locus_tag="VN496_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HXF16754.1"
                     /translation="MVRDASLRSRFVQARSHTLNIARPLTDEDCCVQSMPDASPVKWH
                     LAHTTWFFETFLLEKYEDDFRPFLPAFRMLFNSYYNGVGEKYPRPQRGLLTRPALHEI
                     MLYRKKINERMQRLFEYCGEDPSFLERVELGLQHEQQHQELILTDIKHLLSLNPLQPA
                     YREGESTVSSECSGLQWFSIDGGMVDIGHDGSSFGFDNETPRHKHYLDAYCLASRLVS
                     NGEYMQFIMNGGYHDPRLWLSEGWDWLQSGRIAHPLYWQCSDQGWSEFTLAGRQPLDL
                     ALPAVHLSYFEADAYARWAGARLPSEEEWEHAAQVYEVAEDAIRVDRLHPCTARGPGL
                     QQMFGETWQWTRSSYAAYPGYHWPQGAIGEYNGKFMVNQQVLRGSSCVTPDGHGRITY
                     RNFFPATARWQFSGIRLAKDGPGAS"
     gene            complement(3962..4990)
                     /gene="egtD"
                     /locus_tag="VN496_07105"
     CDS             complement(3962..4990)
                     /gene="egtD"
                     /locus_tag="VN496_07105"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXF16755.1"
                     /translation="MVTAHTDNHLELVRSNSHRAARRTTDALRWRNRETIADSWLGRK
                     ELIAGLTAPHPWISSKYFYDALGSQLFDAITRLPEYYPTRTEAAILARHVNDIARTVG
                     TGRILIDLGAGNCEKAAALFPALRPAQYVAVDISTEYLETALFRLRQQFPGIAMTGLG
                     MDMSQGLSLPDSIPDAGRLFFYPGSSIGNFTPQEATSFLSDLQLQCHRGGGLLIGVDL
                     IKEKAILDAAYDDALGVTAAFNLNALNNVNSVLGSDFDVRDWRHCGFFNPTMSRVEMH
                     LETRLAVEVHWPGGLRCFEAGERIHTENSYKYSVEDFGELLSKAGFTNVTAWTDQRKW
                     FATFYATA"
BASE COUNT         1115 a         1494 c         1571 g         1199 t
ORIGIN      
        1 ctgggctgag tgcctccctc gcggcgaagg aaaagcacct gcgcttcatc acgctcgagc
       61 aggaagattc gcttggcggc accgtatatc actatccacg caataagatc acgatgacct
      121 cggggatgca tgtgcctctg atcggacgca tcaagctagg ggaagtccgc aaggaagcgc
      181 ttttggagtt ttggctcggt gtaatttcaa aagtcggatt gaaggtaaat tttagagaac
      241 aagtaaatgc gatcgaaaag gtaagcggcg gtttcaaagt cacgacttca aagcacagtt
      301 atgtcgcgcg caacgttgtg ctggcgatcg gccgccgcgg cacgacacga cgtctcgacg
      361 ttccggggga acatcaagcc aaagtggtat atcggctgat agatcccgca caatatcgtg
      421 gccagcacgt tctgatcgta ggtggagggg acagcgcgat cgaggctgcc atcgctattt
      481 ccagagaacc gaacacgacg gtttcgctgt cataccgcgg cgcaaccttc aatcgcatca
      541 aatccgcaaa tcaggtgcgc ttgcgcgagg tccagaaaga aggccggctg cgagtcttgc
      601 tggagtcgga gaccgtggaa attcaattgc gcaaaatcgt tctgaagcac aaaaacaacg
      661 tattctccct ccgcaacgac gtgctcatcg tatgcgctgg agggctgctg cctacccagt
      721 tccttcgcaa cttgggtatt ctcgtcgaaa cgcatcatgg tttggcttga gctctattgc
      781 cgctattgat cggtggcgtg tacgcgtttg gagttcggtc ctgattacca tggttacggc
      841 ctttcgaaat tcctgcgacc cgccactgaa agtggaatta catgacagaa cagatcgatg
      901 tcaacgaagg ccgcataacc accgagctgc gtgaccacct tttcctgatc ggtatcgatc
      961 ggccggccaa gctgaacggc tttacgccgg tcatgctgcg tggcatggca caggcctata
     1021 cccgttatga acatgagccg ctggcgcgtt gtgcagtggt attcgcagaa ggcgcacact
     1081 tcacggcggg cctggatttg ccgaaagtcg ctgcgcatct cggcggaaac acgctgctgg
     1141 ttgaaaaaga tgagatcgat ccattcgaat cccgtccccc attccgcagc aaacccgtgg
     1201 tatttgccgt gcagggcatc tgttacacgc tgggtatcga gatgatgctg gccgcggaca
     1261 tcgccgtggc agcttccaat acgcgcttcg cacaagtcga ggtcaaacgg ggattgatgc
     1321 cgaccggagg ggccaccatc cgcatggttc aacgtgcggg ctggggcaat gccatgcgct
     1381 atctgctcac tggcgacgaa ttcgatgcgc agaccgcact tacgctcgga tatattcagg
     1441 agatcgtcga gcccggacgt caacgcgaac gcgcgatgga attggcaacg agcattgccg
     1501 atcaggcgcc gctagccgtc gcagcgacgc tgtcgaatgc gcgtgtggcg ctgcgcgagg
     1561 gtccagaaac cgctgcgtcg ctgttcgggg caacgcagac ccgcttgctg gcgtcggaag
     1621 atttccagga tggcctgcgc agcttcaagg aaaaacgcgc gccgaatttt cgtgggaaat
     1681 agttcaactt gtcgcgggga cacttgtccc gtcccgcaga ctcgctcaca gccggaatga
     1741 cccattgtca ggcttcgtaa tcaccgccgt cttgttcggt gcgttgctgc acgcggcgtg
     1801 gaacgtgttg gtcaaatcgg gcggcgacaa atggctcgac gttgcgttgc ttgcatgcgc
     1861 gtcggcggtt gtttcaagtg ccttgctgcc ctttctgcct tcgcctgctc cggagagttg
     1921 gccgtatatc attgcttcgg ctgtggtaga ggttggttac tttgctttgg tcgcggcagc
     1981 ctaccgaacc ggggaactca gctacgtcta tccgatcatg cgaggcagcg ctcctctgct
     2041 taccgcgctc gcggcgggat tattcgttac agaccggctg acggccggaa actggttcgg
     2101 catcctgctt attagcgccg gcttgctgtt gctgcctgca gaccaatggt ggcgcggtcg
     2161 cttccatgtc gcgtcaacgc tgtttgcctt cagcacagcg gtggtgattt gcatctatac
     2221 acttattgac gggatcggca gccgcttgtc gggtaacgcc ttcacgtatg tggcgtgcat
     2281 gatgtcgctg aacggatttt tgctggcggt atttctattc ctgcaacagc cagcggcagc
     2341 gatgcagcgt attcgtgcaa gctggttgcc gttattgggc ggtggacttt gcgcctgggc
     2401 ttcctatgcc attgcgctat gggccatgac gcgcgcaccg atcgcaatgg ttgccgcgtt
     2461 acgtgagaca tcggtaatct tcggcagcgt acttgctgca gtagtgctca aggaaaaatt
     2521 cagtatggct cgctatgcgg cggcgctgct ggtttgcgcc ggcgccatct cgctgaaggc
     2581 gttgtgagcg attgccgcag aagccggaag ccggtgtttc ggtccactcc tgatcaggaa
     2641 gcgcccgggc catcctttgc caggcggata ccggaaaatt gccagcgcgc tgtggctgga
     2701 aagaaattgc gataggtgat gcggccatgt ccgtccggag tgacgcagga acttccgcgt
     2761 aacacctgct ggttcaccat gaacttgccg ttatattcac cgatggcacc ttgcggccaa
     2821 tggtatcccg gatatgccgc atagctgctt cgggtccatt gccaggtttc gccaaacatt
     2881 tgctgcaggc caggacccct cgcggtgcaa ggatgcagcc ggtccacgcg aatcgcgtct
     2941 tccgcgacct cgtacacctg tgctgcatgt tcccattctt cttcgctggg caggcgggcg
     3001 cccgcccagc gggcgtaggc gtcagcctcg aagtacgaga gatgcactgc gggcaacgcg
     3061 aggtcgagtg gctggcggcc cgcaagagtg aactcgctcc agccctggtc gctgcattgc
     3121 caataaagcg gatgagcaat acggccagac tgcaaccagt cccatccctc cgaaagccat
     3181 aagcgcgggt cgtgatagcc gccattcatg atgaactgca tatattcgcc attgctgaca
     3241 agacgggaag ccaggcagta agcgtcaaga tagtgtttgt ggcgcggcgt ttcattgtcg
     3301 aaaccgaatg atgagccatc gtggccgatg tcgaccatcc cgccatcaat ggaaaaccac
     3361 tgcagcccgc tgcattctga agaaaccgtg ctctccccct cgcgatatgc cggctgcagc
     3421 ggattgagtg acagcaagtg cttgatgtct gtcaaaatga gttcctgatg ttgctgctca
     3481 tgctgcagtc ccagttcgac ccgttccaga aacgacggat cctcgccaca gtactcaaac
     3541 aacctttgca tgcgttcgtt gatttttttg cggtagagca tgatttcgtg caacgcaggc
     3601 cgggtcagca ggccgcgttg tgggcgcgga tatttttcgc ctacgccgtt gtagtacgaa
     3661 ttgaacagca tgcgaaaggc cggcaggaag gggcggaagt cgtcttcgta tttctccagc
     3721 aagaaggttt caaaaaacca ggtggtatgg gcaagatgcc acttcaccgg gctggcatcc
     3781 ggcatggatt gcacgcagca gtcttcgtcg gttagcggcc gggcaatatt gagggtatgg
     3841 gaacgtgctt gcacaaaccg actgcgcaga ctggcgtcgc gaacaattgc agtatctctg
     3901 ccttgcactt gcaaatcgac cgcattcggt cggcgcggat gattcacgat ggcctccttc
     3961 actatgctgt cgcgtaaaac gtcgcgaacc atttgcgttg atcagtccat gccgtgacat
     4021 tggtgaagcc cgccttgctc agcagttcac caaaatcctc gacgctatat ttgtagctgt
     4081 tctcggtgtg aatgcgctcg ccggcttcaa agcagcgcag gccgccgggc cagtgcactt
     4141 caaccgcgag ccgggtttcc aggtgcatct caacacgcga catggttgga ttgaaaaaac
     4201 cgcaatgccg ccagtcgcgg acgtcgaaat ccgaaccgag caccgaattg acattgttta
     4261 atgcgttcaa attgaacgca gccgtcacgc cgagcgcatc gtcataggcg gcatccagga
     4321 tcgctttctc ttttatcaga tccacgccga tcagcagacc ccctccgcgg tggcattgca
     4381 gttgaaggtc cgagaggaaa ctggttgcct cctgcggggt gaaattaccg atggaggaac
     4441 ccggataaaa aaacaaacga ccggcatccg gtatggaatc tggcaacgaa agcccctgcg
     4501 acatatccat tccgagacca gtcatggcga tgcccggaaa ctgctggcgc aaacggaaca
     4561 gggcagtttc cagatattcg gtggaaatat ccactgcgac atactgggct ggcctgagcg
     4621 ccggaaacag agcggcagct ttctcgcagt ttcccgctcc gaggtcgatc aggatgcggc
     4681 ccgtacctac cgtacgtgcg atgtcattga catgacgggc aagaatcgca gcttcggtgc
     4741 gggtggggta gtactcgggc aagcgcgtta tcgcatcgaa caattgcgag ccaagcgcgt
     4801 cgtagaaata cttcgatgaa atccaggggt gtggtgcggt caatccggca attaattctt
     4861 ttcggcctag ccaggaatct gcaatagttt cgcggttccg ccatcgaagt gcatcggtag
     4921 tcctgcgggc cgcccggtgt gaattgctcc gcaccagctc cagatggttg tccgtatgcg
     4981 ctgtcaccat tgggttttct tttttgttga gttgaagcat cttttcaggg gctcctgttc
     5041 gattaggttt tcgagccgcg gagcgaggac catggtagtc cattcccgcc agcgcgtctc
     5101 ccgagcgtgc tcctcacatt cggcaatcaa taccgtctgg gattggtgcg ctaactcatc
     5161 cgaccggctc atggcaaagc ggttccttaa taccccggag agactacgag ttccgtatcc
     5221 gcaaaccaat cgtcgtgccc ggatttgcag tcgtcgcaac gtggcgacag tcccaatcgg
     5281 gtgttgctca gtgaaaagct ttacgagggg ccgcgtgcgc cgtacaccgc tcagtggaat
     5341 gcgttagcgt ttcgggcgtt caataggtcg ccctgccac
//
LOCUS       DATMZQ010000219         7176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3966891, whole genome shotgun sequence.
ACCESSION   DATMZQ010000219 DATMZQ010000000
VERSION     DATMZQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3966891"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VN496_07110"
     CDS             complement(<1..917)
                     /locus_tag="VN496_07110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXF16756.1"
                     /translation="MSGPLAGITVLDLTRVLAGPFATQILSDLGAEVIKIERPETGDD
                     TRGWGPPYVKDANGNDTAETAYFAGCNRGKKSVTLDLADPQAQKRLRQLAGECDVLVE
                     NYKVGDLARYGLDYASLRKLYPGLVYCSITGFGQTGPYAERAGYDPVAQAMCGMMSVT
                     GEPEGQEGTTPQRVGVAVVDLMAGHYAVIGILAALMHRRQSGEGQHIDIGLLDVGVAS
                     MANIASAYLGAGVVSKRNGGVHPSVTPSQVFQCTDGYVIVAAANDGQFRKLCEVAGRP
                     GIAQDKRFATNAARVQNRAVITPLLEDIFR"
     gene            complement(991..1956)
                     /locus_tag="VN496_07115"
     CDS             complement(991..1956)
                     /locus_tag="VN496_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXF16757.1"
                     /translation="MRLHCMTVVAVCVLATAPAFCADYPEHPLRLVVPFTPAGATDVL
                     ARLTGERLGDKLGQTVVIENRPGAGANVGAEFVARSAPDGYTMLMGPASVYAISVTLY
                     PKLGYELTKDLTPVSLVANVPHVLLVNNDVPAASLPELIRLAARQPGMLNIASQGSGT
                     VSHLEAELFKHMAGIDMVHVPYRGSAPALVDLMGGRTQVMFDSIASALPHIRAGKLRA
                     LAVSSKTRSALLPDVPTVDESGLKGYSAHSWLGIFVPAATPRPIVERLQREVAAALND
                     PQIRARLLGAGFEPASSTSAAFAKLVREEIEKWRPIVKMSGATAD"
     gene            2049..2831
                     /locus_tag="VN496_07120"
     CDS             2049..2831
                     /locus_tag="VN496_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXF16758.1"
                     /translation="MQVKAEEAAAALMRDHAAKRKIIPFAKDYGATDLAGAYAIQAAY
                     VKQLEATLGPRVGYKIGLTSKRMQQMCGVNHPNSGVVFANRLYESGAKLSLSQLGRLG
                     IEFECCARLGASLRPRGRPYGLDEVSAAVDAVCPAFEVIDDRNSDYPLDLLSLVADNS
                     WNEGNVLGEFRTTWPDLANTPSILECNGRVIDEGRGRDVLGHPFEPLRWLANNLNELG
                     HTLNKGEIVLTGSWVTTRFPKAGEHYRYTIDGIGSVEVTLTA"
     gene            2920..3465
                     /locus_tag="VN496_07125"
     CDS             2920..3465
                     /locus_tag="VN496_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16759.1"
                     /translation="MNRTRTVHATPSVAALILLAKQWRILLALCLGIFAFTASAQDSR
                     ASSFPQVWLNAGVYSYHFDTEKDLRNNNIGFGAEVALNNNHELLGGTYINSNDGRTYY
                     AAYQWRPLHWDVSGVHVGGGIIIGAFDGYPNYHDGGWFVAPLPVLSIEGRWLGANISL
                     IPTVKDRFDGALAIQLKLRVW"
     gene            complement(3551..4165)
                     /locus_tag="VN496_07130"
     CDS             complement(3551..4165)
                     /locus_tag="VN496_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16760.1"
                     /translation="MARTPINPGRIDWSGENPGMYLKEKADGPFVSLISYFRVVHSPH
                     GKGHAAFMLLDPYGEGRNPKKPNLCLTDNEPLADYVRQGFVTYFGAFKGATGLGHLQV
                     KPAWDFMAGGDGKNLHTEWFRSATGQVHLSWNELGAPFLVEFDKEHSATGKHEMISLF
                     VDSRECGATINGNAVAGQAFPREFAGKKDSSTAFLAFSETWVKA"
     gene            complement(4220..4600)
                     /locus_tag="VN496_07135"
     CDS             complement(4220..4600)
                     /locus_tag="VN496_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF16761.1"
                     /translation="MEHTFKFSVSHTDTSQFKDDGLRPFFQYRDLGIRDATNGAVVAH
                     VIRAKPGAPVHPERHHHEVQFQMVYVLKGWVRFNYEGVGEITLKAGSCVHQPPGIRHT
                     ELAHSDDLELIEIVMPADFETETD"
     gene            complement(4621..5559)
                     /locus_tag="VN496_07140"
     CDS             complement(4621..5559)
                     /locus_tag="VN496_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXF16762.1"
                     /translation="MTKNMKLKGREFAKGLRFPEGPVAMADGSVLVVEIERGTLSRVD
                     QQGRVQIVAQTGGGPNGAAIGPEGKCYLCNNGGFRWHYDADGSMRPAARADDYSGGRI
                     ERVDLSTGNIELLYRECDGVPLKGPNDIVFDGKRGFWFSDLGKVYGRQMDRGAVYYAR
                     TDGSMIREAVFPITTPNGVGLSPDGNTLYVAETETSRLWSFRIIGEGELEKLPWPSPH
                     GGKLVAGLPGYQRFDSMAIEEDGNICVATLMRGGVTVISPAGEVVEFHEAPEHYCTNL
                     AFGGVAMQKAYVTLSGHGNLLEVDWPRPGLRLHYQN"
     gene            complement(5594..6523)
                     /locus_tag="VN496_07145"
     CDS             complement(5594..6523)
                     /locus_tag="VN496_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HXF16763.1"
                     /translation="MLSMLVIAACCVSGATASATPLQDCMQSSVLSACLDAKLKAANK
                     QLNATLKAAQERIEQLQAQGRRPVLGTFIDSQRKFNAYRDAQCGWQGVRAPAGSNSGD
                     YVKDCQIRETLARERQLAEFLAGDDAANAGVDSPAMAEADSPTPKIEVLPSSSSPGPD
                     SAVGDPPKAALPVPDSAAVESPKAAVPSDGNSVPASRGNEWRLVKWVANGAEKTIVGD
                     SDITIAFDPSGKVAGNASLNRFTGSYRFDVDGRLQWPRTGFALTRMAGPAPLMGQERA
                     FLESLRRTTQYRVEGPQLILQSENGGTALIFTR"
     gene            complement(6567..>7176)
                     /locus_tag="VN496_07150"
     CDS             complement(6567..>7176)
                     /locus_tag="VN496_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXF16764.1"
                     /translation="PRIMQPRKFFYDMLDNEVRSAMDGALARLRAAGAPVGEVNVPGI
                     ELAPGIQLITIAAEGAQANAELLARHADKLGEDVRLRLETGQFYLAVDYIKAQQLRNQ
                     VRQSMISCFGDADVMIIPATPVLPPRSGTTILELGGKTVHVAPTLTRFTSPINFCGFP
                     ALSMPCAKSKNGLPINLQIVGRPGADATVLRVARWCEQVFAA"
BASE COUNT         1486 a         2180 c         1986 g         1524 t
ORIGIN      
        1 cgaaagatgt cttcgagcag aggggtaatg acggcacggt tttgcacacg cgcagcgttt
       61 gtcgcaaaac gcttatcctg cgctattccg ggccggcccg cgacttcaca aagtttcctg
      121 aactgtccat cgttggcggc cgcaacaatg acatatccat ctgtgcactg gaacacttgc
      181 gatggcgtta cgctcggatg cacgccaccg tttcgcttcg acacgacacc agcaccaaga
      241 taggcagacg cgatattggc catcgacgct acgccgacgt ccagcaatcc gatgtcgata
      301 tgctgccctt caccgctttg cctgcgatgc atcagtgccg caagaatgcc gatcacggcg
      361 tagtgacctg ccatcaggtc gaccaccgca acacctacgc gttgcggcgt cgtgccttcc
      421 tgtccttcgg gctctccggt gacgctcatc atgccgcaca tggcttgtgc gacaggatca
      481 taaccggccc gttccgcata cggaccggtt tggccgaatc cggttatcga gcagtacacc
      541 agccccggat ataattttct cagggacgca tagtcgagcc cgtaacgcgc caggtcgccc
      601 accttgtagt tctccacgag gacatcgcat tctccggcca gttggcgcaa ccgtttctgt
      661 gcttgcggat ccgccaggtc gagcgtcacc gatttcttgc cacgattaca acccgcaaag
      721 tacgcggtct ccgcggtatc gttgccgttc gcgtctttta catacggagg cccccacccg
      781 cgcgtgtcat cgcccgtttc gggccgttct atcttgatta cttcagcgcc caggtccgag
      841 aggatctgtg tggcgaacgg tcccgccagc acgcgcgtga gatctaatac cgtgattcca
      901 gccagtggcc cactcatgac ttctccctaa cggcaaaact ttgaagcgcg cttcatcttg
      961 cactatgcgt cggatatcaa ccaaggtgcg tcagtccgcc gtcgctccag acatctttac
     1021 gatcggacgc catttttcga tctcctcacg caccagtttc gcaaacgcgg cggacgtact
     1081 gcttgcgggc tcaaaacctg cgcccaaaag ccgtgcccgg atttgcgggt cattcagcgc
     1141 agcagcgacc tcacgctgca agcgctcgac gatcgggcgc ggcgttgccg ccggtacgaa
     1201 aatacccagc caggaatgcg cgctgtaacc tttcaagccc gactcatcga ccgtaggcac
     1261 gtctggcaat agcgctgagc gggttttgga ggaaaccgcg agggcccgca atttccctgc
     1321 tcgaatgtgc ggaagcgctg aagcgatact gtcgaacatg acctgggtgc gcccccccat
     1381 aagatcgact agcgcgggcg cgcttcctcg atagggcacg tggaccatgt cgatgccggc
     1441 catatgcttg aacagttccg cttcaagatg ggataccgta ccgctacctt gcgaagcgat
     1501 attgagcatt cccggctgcc tggccgccag gcgaatcaat tctggaagcg acgctgccgg
     1561 cacatcgttg ttgaccaaca gcacatgcgg aacattggcc acaagcgaca ccggcgtgag
     1621 atccttggtc agctcatagc caagtttagg atacagcgtg acggaaattg cgtataccga
     1681 agcagggccc atcagcatgg tgtaaccgtc cggcgcagag cgggccacaa actcggctcc
     1741 aacgttggcc cccgcaccgg gacgattctc gattaccacg gtttggccca gtttgtcgcc
     1801 caggcgttcg ccagtcaggc gcgccagcac atccgtggcc ccggccggcg taaaaggcac
     1861 taccaaccgc aatggatgct ccgggtagtc ggcgcaaaat gccggcgccg ttgcgagcac
     1921 gcaaaccgca accaccgtca tacagtgcaa tcgcattggt gcctttgctt cgggcggcgc
     1981 atttgccacg cgagaagcca aggataccga gagccgatat gatgtcgcaa tagacgagga
     2041 gacgatgtat gcaggtcaag gcagaagaag cagcggccgc gctgatgcgc gatcacgcgg
     2101 caaaaagaaa aatcattccg tttgcaaagg actacggcgc aaccgacctt gcaggtgcgt
     2161 atgcaatcca ggccgcgtat gtcaagcagc tggaagcaac gctaggcccg cgcgtcggct
     2221 acaagatcgg actgacctcc aaacgcatgc agcaaatgtg cggcgtgaac catcccaact
     2281 ccggggtggt gtttgcaaac cggctgtacg agagcggtgc gaaactttca ttgtcgcagc
     2341 ttggccgtct cgggatcgag ttcgaatgct gtgcgcggct cggcgcctcg ctgaggcccc
     2401 gcggccgtcc atacgggctg gacgaagtgt ccgccgcggt tgacgcggtg tgcccagcat
     2461 tcgaagtcat cgacgaccgc aattccgact atccgctgga cctgctttcg ctggtggcgg
     2521 acaactcctg gaacgaaggc aacgtacttg gcgagttccg gacgacgtgg ccggatctcg
     2581 caaatacgcc cagcatcctt gagtgcaatg gcagagtcat cgacgaagga cgcgggcgcg
     2641 atgtgctggg ccatccattt gaaccactgc gctggctggc caataacctg aacgaactcg
     2701 ggcacacatt gaacaagggg gaaattgtcc tgaccggcag ctgggtgact acccgttttc
     2761 cgaaagccgg ggaacattac cgctacacca tcgacggtat cggttctgtc gaggtcacgc
     2821 tgaccgccta gttgagaaca atcgtccgcg gcgcaacatc ggggcgcgta ccaataccgc
     2881 cctgtgttcc gatggagacg ccatcccttt gaggccagat tgaaccgcac aagaacggtc
     2941 cacgcaacgc cgtccgtcgc agcgttgatc ctcctggcca aacaatggcg catcttgctc
     3001 gcattgtgcc tgggcatctt tgcgttcacg gcctcagccc aggactcacg ggcaagttcg
     3061 tttccgcaag tctggcttaa cgccggcgtc tattcatatc acttcgacac ggaaaaggac
     3121 ttgcgcaata acaacatcgg ctttggcgcc gaagtggcgt taaacaacaa tcatgaattg
     3181 ctggggggga cctacatcaa cagcaatgat ggacggacgt actatgctgc ctatcaatgg
     3241 cgtccgctcc actgggatgt gagtggggtt cacgtcggcg gcggcatcat catcggcgct
     3301 ttcgacggct atccgaacta ccatgatggc gggtggtttg tggcccctct gccggtgcta
     3361 tccatcgagg gcaggtggct tggggccaat atcagcctca tacccacggt aaaagatcga
     3421 ttcgacggcg cattggcaat ccagctaaag cttcgggtgt ggtaatacgg gaaacaagtt
     3481 catcgaacga tctttcacgc agtcaaggct cggctggaag ggtatatctc ctcacgaaca
     3541 tcaaacgccc tcacgccttc acccaagtct cgctgaacgc cagaaacgca gtgctactgt
     3601 cttttttacc ggcgaattcg cgtggaaaag cctggccggc aaccgcattg ccattgatgg
     3661 ttgcgccaca ttcgcgcgaa tcgacgaaca gtgaaatcat ttcatgcttg ccggtcgcgg
     3721 aatgctcctt gtcgaactcc accaggaacg gcgcaccgag ctcgttccac gatagatgaa
     3781 cttgtcccgt agcggagcga aaccattcgg tatgcaggtt cttgccatca ccgcccgcca
     3841 tgaaatccca agccggcttt acttgcaggt ggccaaggcc tgtcgcacct ttgaaggcgc
     3901 cgaagtacgt tacgaaacct tgcctgacat agtcagccag cggctcattg tccgtcaggc
     3961 aaagattggg cttcttcgga ttcctgccct cgccgtaggg atcaagtagc atgaaggccg
     4021 catgtccctt gccatgtggc gagtggacga cgcggaaata gctgatgagt gagacaaaag
     4081 gtccgtctgc tttttccttc agatacatgc ccgggttttc tccggaccag tcgatcctgc
     4141 ccggattgat tggtgtgcgt gccacgtgtt ctcccctttt ttgagtgaat tgtccgtgct
     4201 gccgatcgct ggatcgtctt cagtcggtct cggtttcgaa atccgccggc atgacgattt
     4261 caatgagttc gagatcatct gaatgcgcca gttcggtgtg ccggatcccg ggcggctgat
     4321 gcacgcacga gccggctttc agcgtgattt cgccgacacc ttcgtagttg aacctcaccc
     4381 atcccttgag tacatacacc atctggaact gaacttcatg atgatgccgc tccggatgca
     4441 ctggcgcccc cggcttcgcg cgaatgacgt gcgcgaccac cgcgccgtta gtggcatcgc
     4501 gaatgccaag atcccgatac tgaaaaaaag gacgcagccc gtcatccttg aactggctgg
     4561 tatcggtatg gctcacagaa aatttgaagg tatgttccat tcgatgactc cttccagatt
     4621 ctagttttga taatgcagcc gcaacccggg gcgcggccaa tcgacttcca gtaaattgcc
     4681 gtggccagac agggtcacgt atgccttttg catggcaaca ccgccaaacg ccagattcgt
     4741 gcaatagtgt tccggtgctt cgtgaaattc gacaacttcg ccagctggtg aaatgacagt
     4801 aacgccgccg cgcatcagcg tggcgacgca tatgttgccg tcctcttcga ttgccattga
     4861 atcgaaacgc tgatagcccg gcaggcctgc caccagcttt cctccatgcg gggacggcca
     4921 cggcagcttc tctagttcac cttctccaat aatccggaat gaccacaggc gactggtttc
     4981 tgtttctgcg acatacaagg tatttccatc gggagagagc cccacgccgt ttggcgtggt
     5041 gattggaaac acggcctccc tgatcatgct gccgtccgtg cgcgcatagt aaaccgcacc
     5101 gcgatccatc tgacgcccgt acaccttgcc aaggtcgcta aaccaaaagc cccgcttacc
     5161 atcgaatacg atgtcattgg gcccttttag cggcacgccg tcgcattcgc ggtagagcaa
     5221 ttctatgtta ccggttgaca agtccacccg ctcaattcgc ccgcctgaat aatcgtcggc
     5281 acgggcggcg gggcgcatgg atccatccgc gtcataatgc cagcgaaatc cgccgttgtt
     5341 gcacaggtag catttgccct cggggccgat tgcagcgccg tttgggccac cgcctgtttg
     5401 tgcgacgatt tggacacgcc cttgttggtc gacacgggac aaggtgccgc gctctatttc
     5461 cacgaccagc actgacccgt ccgccatggc aacgggacct tccggaaagc gcaggccctt
     5521 ggcgaactca cgtcctttga gtttcatgtt cttggtcatc gcgcggtccc gttggtggtt
     5581 atttttgtgt catttaccgt gtgaagatca gcgcggtccc accgttttca ctttgcagaa
     5641 tgagctgcgg cccctcgacc cgatactgcg tcgtgcgtcg tagcgattcc aagaacgcgc
     5701 gctcctgtcc catgagcggc gctggtccgg ccattctggt aagggcgaat cctgtccgcg
     5761 gccattgcag gcgcccatca acgtcaaacc ggtaactgcc ggtaaagcga ttcagcgaag
     5821 cgttgcccgc aacttttcca gaaggatcga aagcaatcgt gatatctgaa tcaccaacga
     5881 tggttttttc cgcaccgttc gccacccatt tgaccaggcg ccattcgtta cctcgagacg
     5941 ccgggacaga attgccgtct gaaggtacgg cggctttcgg cgactcgacc gcagccgaat
     6001 cgggtaccgg cagggctgct ttcggtggat cccccacagc agaatccggc cccggcgaac
     6061 tcgatgaagg gagcacctcg attttcggcg tgggcgaatc ggcttccgcc attgcaggcg
     6121 aatcaacgcc ggcattggcc gcatcgtcgc cggcgaggaa ctcagccagc tggcgttcgc
     6181 gcgccagtgt ttcgcgaatc tgacagtcct tcacatagtc accgctattg gagcccgcag
     6241 gcgcacgcac accctgccag ccacattgag catcgcgata tgcgttgaac ttccgttggc
     6301 tatcgataaa tgtgccgagc accgggcgtc tgccttgcgc ctgcaactgc tcgatgcgct
     6361 cttgtgcggc tttcagcgtg gcgttcaact gtttgttggc tgctttcagc ttggcatcca
     6421 gacacgcgga aagcacactg gactgcatgc aatcctgcaa tggcgttgcg gaagccgtgg
     6481 ctccggaaac gcagcatgcg gctatcacta gcatggataa cattctcact gacttgctcc
     6541 tgttggtttg ccgtaatgca accggttcat gcagcgaata cttgctcgca ccagcgcgcg
     6601 acacgcaaga cggtcgcatc agcgcccgga cgacccacga tttgcaggtt gatgggcaag
     6661 ccatttttag atttcgcgca cggcatcgac agcgcaggga acccgcaaaa attaatcggg
     6721 gaagtgaagc gcgtcaatgt cggcgcaacg tgcaccgtct tgccgcccag ttccagtatt
     6781 gtggttccgc ttctcggcgg cagcacgggc gtcgctggaa taatcatgac gtccgcatcc
     6841 ccgaagcagc tgatcattga ttggcgcacc tggttccgca actgttgcgc cttgatataa
     6901 tcaacggcca gataaaactg acccgtttcc aggcgcaatc gcacatcttc gccgagtttg
     6961 tcggcatgcc gggccagtag ttctgcgttc gcttgcgcac cttctgcggc gatggtaatc
     7021 agctgaatgc ccggtgcaag ctcgattccg ggtacgttga cttcgccgac tggtgcacca
     7081 gcggcacgca agcgtgctag cgcaccatcc atcgcgcttc gcacttcatt gtccagcatg
     7141 tcatagaaga acttccgcgg ctgcatgatg cgcggc
//
LOCUS       DATMZQ010000220        15090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1204429, whole genome shotgun sequence.
ACCESSION   DATMZQ010000220 DATMZQ010000000
VERSION     DATMZQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204429"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VN496_07155"
     CDS             complement(<1..972)
                     /locus_tag="VN496_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXF16765.1"
                     /translation="MPVLPEPFPLLIVSASGRALAQSASRMGLKAVVLDLFDDMDTRA
                     LADASQSVAGRNARFDARKLLAAAQALSPPERCSGLVYGSGFEGRTRLLVRLARGRRL
                     FGNAPETIAQVKDPLRFFAMLDSLDIAHPEFRLEAPPEVTGWLTKRSGGAGGSHVKPM
                     RRRDRARPQRYFQKFQTGRTLSVLFLADGHNVRVIGFNEQWCADVAQCALYCYGGAVS
                     GIPVTPALRACMAAAVEKLVRATGLVGLNGLDFILDADDLPHVLEINPRPTATIDLYD
                     ADFPAGLLALHLRACHGELPQLLPSQHRSRAHAIVYAAENLVVPAGVK"
     gene            complement(956..1864)
                     /locus_tag="VN496_07160"
     CDS             complement(956..1864)
                     /locus_tag="VN496_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HXF16766.1"
                     /translation="MEKPHILHLFTATEQVSPFDVNMAYDAGYDAVVPYTNVSLEQVA
                     ALTQDAIFSRGPKGVRRTGLFLGGRATGLAADMLDAARRAMVPPFEVSVFVDPSGAFT
                     TAAAMIAAVEKQLAKAHAGGLEGKTVILFGGSGPVGTAAAVLSAKAGANVGIVAREDA
                     TRARNTAQECNRRYGVRIEAVEANTPALKKAMMQRAHIVLATAKAGHRVVGSELLADA
                     RALLVAADVNAVPPSGVEGLDAMDDGKALQSGSGKAVGIGALAIGNIKYQTQRGLLKT
                     MLEADKALYLNLPEALQEARKHAGIA"
     gene            complement(1878..2858)
                     /locus_tag="VN496_07165"
     CDS             complement(1878..2858)
                     /locus_tag="VN496_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HXF16767.1"
                     /translation="MRTVSVTAPARLHLGFLDLNGSRGRRFGSVGLTLEGPCVELSVQ
                     RAPRFSVAGSQAERAAQFAKAVCRRFNFPDHVQLTIAQTVPEHVGLGSGTQLGIAVGV
                     ALSKFHGHDIPVRQIATTVQRGQRSGIGAAAFELGGFLVDGGKGPTDDPPPIISRIEF
                     PTDWRIVLVLERAARGLHGELETAAFQALPEFPETMANYLCRLMLMQALPALVEHDID
                     RFGNAIGELQRITGDYFSPAQGGRFFSPVVAEALGWIASHDIAGVGQSSWGPTGFAIV
                     GSAERANELVNAANERWGNAGPLQFAVCRGRNRGGDVASSQSAAASVKIN"
     gene            3081..4355
                     /locus_tag="VN496_07170"
     CDS             3081..4355
                     /locus_tag="VN496_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16768.1"
                     /translation="MSDPVPAQEFENVPCPFCGLLCDDLRISIGGGSINVSANGCARS
                     RRLFSAQAGADTGCAIDGRSASFDQALSRATGILRQARRPLMLSAGTDVAGMRELVEL
                     AELSGGIVDHVNRETMFRNLRVLQDTGWISTTLTEIRNRADLLIIAGNAVSSRFPRFF
                     ERCFGPFDTLFETGARELIFLGSLPDDVPEVLKARTTVFPFGLDRLAEVIAFLRALLA
                     GRPMRASDVAGLSLEQLSSLVARMRAAKYGVISWTASEFNFAQADLAIQAICDLVRDL
                     NRETRFSILPLGGSDGDITAQQVTTWQTGFPVGVDFRAGAPSQASSSTHASILTGRSE
                     VDALLFVSAFDSDKTPPATNLPTIVLGRAGMQPGNCAAFIPIGVPGLHHSGHLYRTDN
                     VVAIRMRQIAKSAYPSAAQVLRKILDACRETT"
     gene            4352..6016
                     /locus_tag="VN496_07175"
     CDS             4352..6016
                     /locus_tag="VN496_07175"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HXF16769.1"
                     /translation="MIRLAGGRLYDPVNGIDGIVRDVYMDRGRIVPAPAAGGRIDTEY
                     DVQGRVIMAGAIDLHTHIGGGKVNIARAMLPEDHRNSAFARTGMTRAGSGVVAPSTFA
                     AGYRYAEMGYTACFEPAMLPVNARHAHMEMADTPIIDKGGYALLGNDDFLLRMLAAGA
                     EQKLINDYVAWTLHATQCIAIKVVNPGGISAFKFNARSLDVDEPHPHYRVSPRAILTA
                     LTRALHELRVPHPLHVHASNLGVPGNVATTLATMEAAEGLPMHLTHIQFHSYGTEGGR
                     KFSSAAADIAQALERHANVSADVGQIIFGQTVTASGDTMAQYRNAPHAHPAKWLCMDI
                     ECDAGCGVVPFRYRDANFVNALQWAIGLELFLRVKDPWRLFLTTDHPNGGPFTSYPHL
                     IRLLMDCNFRNEKLAQIHPEAAAHSQLSGLDREYSLYEIATMTRAAPARSVGLADRGH
                     LGIGAAADVTVYRDIADREQMFAAPQYVFKDGELVARDGRIVKVTRGATHVVRPDFDR
                     GIEKQLRDYFERYHTIRFDNFPLSSEELTEFGSRETLVHPCSRPIS"
     gene            6013..6906
                     /gene="fhcD"
                     /locus_tag="VN496_07180"
     CDS             6013..6906
                     /gene="fhcD"
                     /locus_tag="VN496_07180"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HXF16770.1"
                     /translation="MKLNGVAIDDTFAEAFGMRATRILITALNLKWAYHAAHSLTGFA
                     TSVIGCGCEAGIERELLPDETPDNRPGIAVLLFAVSSKELAKQIERRVGQCVLTSPSS
                     AVYAGLEGEQVSLAKNLRFFGDGFQMSKVIGSQRYWRVPVMDGEFLGEETTGMIKAVG
                     GGNFLVLARGQSEALEACEAAIAAMSKVRGVIMPFPGGIVRSGSKIGSKYKALSASTN
                     SAFCPTLRGAVKSELTPETGAVLEIVIDGLSESDVRNAMRAGIRAACSLGPTGGVQRI
                     SAGNYGGKLGPFHFRLHDILQ"
     gene            6903..7643
                     /locus_tag="VN496_07185"
     CDS             6903..7643
                     /locus_tag="VN496_07185"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HXF16771.1"
                     /translation="MTPLILTLRATAPFDIDLSAVTPDRLTGVKVADVALTGGRQLGE
                     LFDITGEDSQNIIIRNPDERLTHIGAGMREGVLTVEGNCGAYAGLAMRGGMLIVKGNA
                     GDFAGAAIAGSRQGMRGGTIAIYGNVGDRVGERMRRGLVLIGGDAGAYCGANMLAGTI
                     LVAGKVGLMPGFLLKRGSLLLSRAPNPLPATFQDSGEHSLLFLTLLEKRLQREGPTLA
                     HFLPVARTVRRYCGDMACGGTGEMLVFI"
     gene            complement(7652..7960)
                     /locus_tag="VN496_07190"
     CDS             complement(7652..7960)
                     /locus_tag="VN496_07190"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HXF16772.1"
                     /translation="MNKLSCLWFTLALALAAPVFAAVDCSRAKTNSEKLLCSNSRLAS
                     ADQRMASAFRAAIKRGIQPEVLMESQRSWMHDVRDVCNDVECMLRAYETRTSELEDLQ
                     "
     gene            complement(7960..8688)
                     /locus_tag="VN496_07195"
     CDS             complement(7960..8688)
                     /locus_tag="VN496_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HXF16773.1"
                     /translation="MLFKHKVHVLYRKEELDHERLEGKVAIVLDVLFATSTIIAALAN
                     GATEVIPTLDEAGARAEAQKHPQGSYILAGELYAETLPGFASPTPLALMEEKIAGRKL
                     IYSTTNGTVALRQSSRADHVYAAALVNGEAVVDLVLGKHPGKTILIVCSGSMGMVNLE
                     DLYGAGYLVDLLSRALDENSDFSDAALAVRSLFRNEDAADCLLRARVGRMMIERNLIH
                     EVHYAAGLSILQAVPLLQDGRLTI"
     gene            complement(8691..9155)
                     /locus_tag="VN496_07200"
     CDS             complement(8691..9155)
                     /locus_tag="VN496_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXF16774.1"
                     /translation="MTTRYWEDFHVGEVEQIGRRLVDKAEVIAFATQFDPQPFHIDEA
                     AAKRSMYGGLIASGWHTCAMVMRMMCDAYLAQAASVGSPGIDHLKWLKPVRPGDIISA
                     RRTTLETRASKSKPDIGIVNNLWEVFNQEGELVMSMQGYGMFRRRSPGSADK"
     gene            complement(9226..10413)
                     /locus_tag="VN496_07205"
     CDS             complement(9226..10413)
                     /locus_tag="VN496_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF16775.1"
                     /translation="MDFNLTEEQAMLKESVDRFLAKNFSFEQRRQMLADRQPMNAQLW
                     QGLAELGVLSVPFLPDDGGFGGGGVETMLVMQALGRNLVLEPYLATVVLAGSCVALGA
                     AEWQKKILLPKIIDGSMIMAFAHTEPDARFDLGQVKTRATRDGNNFVLEGEKSLVLHG
                     SVADKFIVSARTSGNDADPRGVTLFIVEADEENLGGQEYPLFDGTRALDLQLSGVKLS
                     PESVIGTLDQASPLIDVALDRAMAALCAESVGIMDALHAATVEYLKTRQQFGQPIGRF
                     QALQHRAVDMLVQLEQARSMALLAASKVDSNDIGERRRTVAAAKELTGRAGRFLGQQA
                     IQLHGGMGMTDELNVSHYFKRLTAIDVLFGNAASQRTRFATMKHGETSAQTQPPRKSW
                     VKI"
     gene            complement(10544..11740)
                     /locus_tag="VN496_07210"
     CDS             complement(10544..11740)
                     /locus_tag="VN496_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF16776.1"
                     /translation="MDLNYTTEETAFRAEVRDFIRGHLPGDLSRKVLEHKRLGKEDYV
                     RWQKILFEKGWIAAGWPVAYGGTGWSAIQRFIFEDESAAAGAPRLTPFGLHMVGPVII
                     AFGTDQQKQHYLPRILSSQDWWCQGYSEPGSGSDLASLKTRAQREGDKYIVNGQKTWN
                     THGQWADMCFCLVRTSNESKKQDGISFLLIDMHSPGVTVRPITTLDGEHEVNEIWFEN
                     VQVPVQNLVGEEGRGWTCAKFLLSHERTGIAGVGASKRELHYLKHIATQEQEGGRPLM
                     DDPLFRDRVARVEIDLMALEITCLRVASADRDGKAPGPEASILKIKGTEIQQELTELM
                     MDAVGPHALPHLPMAWGDHWIGAHVGPDYAAPLAARYFNYRKTTIYGGSNEIQRNIIA
                     QMVLGL"
     gene            11927..13273
                     /locus_tag="VN496_07215"
     CDS             11927..13273
                     /locus_tag="VN496_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXF16777.1"
                     /translation="MPDEKALRHLNTSLNLDPLWIPFTANRQFKAAPRLLVSAKDMHY
                     QTHDGRKILDGIAGLWAVNAGHCRRPIVDAIKQQAETIDYATGFQMHHPAAFQAAEKL
                     VAITPPGLNRVFFCNSGSEAVDTALKVALAYHRVRGEGTRRILIGRERGYHGVGFGGI
                     SVGGMVGNRKTFGNMLGSVDHLRHTHDPARNAFSRGLPEHGAELADDLERLVQLHDAS
                     NIAAVIIEPVAGSTGVLLPPKGYLQRLRSICDKYGILLIFDEVITGFGRTGKAFAAQT
                     FGVTPDLITCAKALTNAAVPMGGVIVKQGIYDTFMEKGGPDSAIEFFHGYTYSGHPLA
                     AAAACASLDLFKAEKLFERVAELAPYWEDAAHSLKGLPHVIDIRTIGLVAGIELQPMA
                     EKPGARAFDAFLRAYDKNVLIRTTGDTIALSPPFIIDKAQIDQLFQTVRDVLKALP"
     gene            13327..14835
                     /locus_tag="VN496_07220"
     CDS             13327..14835
                     /locus_tag="VN496_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HXF16778.1"
                     /translation="MKLVIAQMQHETNTFSPVATPWEAFGNNGPLLGEAAGQAVHGTN
                     FAMAAFLDLAREAGAQVVTPVAAWANPSGPVDGAAYDRICGAICEAVAQGCDAVLLDL
                     HGAMVVADRTDDGEGTLLEKVRALAPRTPIAVSLDLHANVTERMVRNCDVITGYKTYP
                     HVDQYQSGELAGSLLLRMMRGEIKPVMAWGNAPVLAQTLRQNTAEGAMKDFVEAARAA
                     ERNGLLAATAFGGFQMADIHDAGLSVVTVSDANRAAAETACNAILDVAWAQKEDFIYR
                     GQPLQEAIAQARRMGEQSGGPILLLDHADNCASGSTQDTMYVLKEAMRQGLSGIAVAP
                     IRDPEAVGKMINAGIGARVTLAVGGKMDMPSIRRKGEPLTLTGVIRAITDGEYTITGP
                     QFTGMRCFMGKTAVLDTGVAQIIVSERNQEPWDRGVYESVGIDPARQRYLLLKSRMYF
                     RPVFLPIAKGMVFCDAPGVSSSDWTEFEYRKLRRPIYPLDEHFANGRNAMAA"
     gene            14856..>15090
                     /locus_tag="VN496_07225"
     CDS             14856..>15090
                     /locus_tag="VN496_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXF16779.1"
                     /translation="MNKPLNQAAVLEELHHYIGGAEVAGRSGHYGDIYNPALGSVKAR
                     VPFATAAEVSAAVDAAQKAFPAWAATPPLRRARV"
BASE COUNT         3020 a         4644 c         4426 g         3000 t
ORIGIN      
        1 tttcactcca gccggcacga cgaggttttc tgcggcatag acaattgcat gcgcgcgaga
       61 ccggtgctgc gacggaagca gctgcggcaa ttcgccgtgg caggcacgca ggtgaagcgc
      121 gagaaggccg gccggaaagt ccgcgtcgta caggtcaatg gtcgctgtgg ggcgtggatt
      181 gatttccagc acatgcggca ggtcatctgc atcgaggata aagtcgagcc cattcaggcc
      241 gaccagtccg gtggcgcgca ccaatttctc gactgctgcg gccatgcacg cgcgcaaagc
      301 tggcgttacc ggtatgccgc tgaccgcccc gccatagcag taaagcgcgc attgagcaac
      361 atctgcgcac cactgctcat tgaagccgat cacccgcacg ttgtggccgt cggccagaaa
      421 cagcacggac agtgttcggc cggtttgaaa cttctggaaa tagcgttgcg gccgcgcacg
      481 atcgcgccgg cgcatcggct tcacatgact tcctccggcg ccgccgctgc gcttggtcaa
      541 ccagccggtg acttccggcg gagcctccag acggaattca ggatgcgcga tgtccagtga
      601 atcgagcatc gcaaaaaaac ggagtgggtc cttgacctgc gcgatcgttt ccggagcgtt
      661 gccaaacaaa cgacgcccac gcgccaaccg caccagaagg cgcgtgcgac cctcgaaccc
      721 tgagccgtat accagtccgc tgcaccgctc gggtggagaa agagcctgcg cagccgccag
      781 taattttcgt gcatcgaatc tggcgttgcg ccctgcgacg ctttgacttg catccgcgag
      841 cgcacgcgtg tccatatcgt cgaacagatc gagcaccacg gctttcaagc ccattctcga
      901 cgctgactgc gccagggcac gccccgaagc ggagacaatc agcagcggaa acggttcagg
      961 caataccggc atgcttgcgt gcctcctgca gggcctcggg caaattcagg tatagcgcct
     1021 tgtcagcttc aagcatggtt ttcaataaac cgcgttgcgt ttgatatttg atattgccga
     1081 ttgcaagggc acctatgcca actgctttgc ccgaacctga ttgcagcgct ttgccatcgt
     1141 ccattgcatc gagcccttcg actccggaag gcggtaccgc attgacgtcc gccgccacca
     1201 gcagggccct tgcatcggcc agcagctcgc tacccaccac acgatgcccg gcctttgccg
     1261 tcgccaatac aatatgcgcc cgttgcatca tcgccttttt caaagccggc gtatttgctt
     1321 cgaccgcctc gatgcgaacg ccatagcggc ggttgcactc ctgtgcggta ttgcgcgcgc
     1381 gcgtggcatc ttcacgcgcg acgatgccga cgttggcgcc tgcttttgca gacagtacag
     1441 ccgccgcggt gccgaccggt ccgctgcctc cgaagaggat gacagtcttg ccctccagac
     1501 cgccggcgtg cgccttggcg agctgtttct ctactgcagc aatcatcgcg gccgcggtgg
     1561 taaacgctcc actcggatcg acgaataccg atacttcaaa cggaggcacc attgcgcgtc
     1621 gcgcagcgtc aagcatgtcg gctgccagcc cggttgcacg cccgccgagg aataacccgg
     1681 tgcgtctgac gcctttcggt ccacgcgaga atatcgcgtc ctgcgtaagc gctgccacct
     1741 gttcgagact gacattcgtg taaggcacta cagcgtcata gccggcatcg taggccatgt
     1801 ttacgtcgaa cggactgact tgttccgtcg cggtgaacag atgaagaata tggggttttt
     1861 ccacgcgccg attcctgcta attgattttt accgatgcgg cagccgactg gctcgatgcc
     1921 acgtcgccgc cgcgattgcg gccacggcac accgcgaatt gcagcgggcc cgcgttgccc
     1981 caacgctcat tggccgcatt caccagttcg ttggcgcgtt ccgcactgcc cacgattgca
     2041 aagccggtcg gcccccatga actttgccca actccggcaa tatcgtggct ggcgatccag
     2101 ccaagcgctt ccgcgaccac cgggctgaaa aatcgcccgc cctgcgccgg agaaaaataa
     2161 tcaccggtga tgcgttgcag ttcaccgata gcgttgccaa agcggtcgat atcgtgctcc
     2221 accagcgcgg gcagcgcctg catcagcatc aaccggcaaa gataatttgc catcgtctcc
     2281 ggaaactctg gtaacgcctg gaatgcagcc gtttcaagct cgccatgcaa tccacgtgct
     2341 gcgcgctcga gaaccagcac aatgcgccag tcggtcggaa attcgattct cgaaataatc
     2401 ggaggcggat catcagtcgg ccccttgccc ccatcgacaa ggaatccgcc aagctcaaaa
     2461 gccgccgcgc caattccgga gcgttggccg cgttgcacgg tcgtcgcaat ctggcgcacg
     2521 ggaatatcat ggccgtgaaa cttcgacagt gccacgccga cggcaatgcc caactgtgtt
     2581 cccgatccca gtccgacatg ttctgggacg gtctgggcaa tggtcagctg aacatggtct
     2641 ggaaagttga accggcggca caccgccttg gcgaattggg ccgcgcgttc agcctgcgaa
     2701 cccgctacag aaaaccgcgg cgcgcgctga acggacagtt ccacgcacgg cccctcgagc
     2761 gtcaggccga cgctgccgaa ccgtcttccc ctcgatccgt tcagatcgag aaatccaagg
     2821 tgcaatctgg ccggggcagt gacggaaacc gtgcgcacac tttcctcatc atcttgatgc
     2881 ccgtatacac atcgggtctt gtggcgagct aacgcgtacc atgacgcgat cagttaaagt
     2941 ttacctcgca ggcccgccag cggcagcgaa aagtttattg caacagtgtc cacattcgaa
     3001 gcattaccat gcgatatgta cagttatgcg acaccatgac cgcgcatcgg ctggaaaacg
     3061 tatagattcg atcgcgcgcc atgtctgatc ctgtcccggc acaagaattc gaaaatgtcc
     3121 cttgcccgtt ttgcgggctg ctgtgtgatg accttcgtat atcgatcggc gggggatcga
     3181 tcaatgtgag cgcaaatggc tgtgcacgaa gccgtcggct gttctccgcg caggcgggtg
     3241 cggatactgg ctgcgcaatt gatggccggt cggccagctt cgatcaagcg ctatcgcgcg
     3301 caacaggtat tctgcgacag gcaaggcggc cgctgatgct atcggccgga accgacgtag
     3361 ccggtatgcg cgaattggta gagctcgcag agttgtcggg tggtatcgtc gatcatgtaa
     3421 accgcgaaac aatgtttcgc aatctgcgag tgctccagga cacgggctgg atcagcacga
     3481 cgttgaccga aattcgaaat cgtgcagacc tgctgatcat tgcagggaat gctgtcagca
     3541 gtcgctttcc gcgcttcttc gagcgctgtt tcggcccgtt cgacacgttg ttcgaaaccg
     3601 gggcgcgcga actgatcttt ctgggttcac tgcccgacga tgtcccggag gttctgaagg
     3661 cgcggactac cgttttcccg tttggcctcg accggcttgc ggaagtgatc gcgttcctgc
     3721 gcgctttgct ggctggacgc cctatgcgag ccagcgacgt ggcgggacta tccctggagc
     3781 agctttccag tctggtcgcg cgcatgcgtg cagccaaata tggcgtgatc agctggaccg
     3841 cctcggaatt caattttgcg caggccgacc tggccataca ggcgatctgc gatttggtca
     3901 gggatctgaa tcgggaaacg cgatttagca ttttgccgct cggcggcagc gacggcgata
     3961 tcactgcgca gcaagttacg acctggcaaa ccggttttcc ggtgggcgtg gatttccgcg
     4021 ctggcgcgcc gtcgcaagct tcatcaagca cgcacgccag cattctcacc gggcgcagcg
     4081 aagtggatgc cctgctattc gtttccgcct tcgatagcga caagactccg cccgccacaa
     4141 acttgccgac catcgtactt ggacgcgccg gcatgcaacc tggcaactgc gcggctttca
     4201 ttccgatcgg cgttcccggg ctgcatcact ccggtcatct gtaccgaacc gacaacgtgg
     4261 ttgcaatacg catgcgccaa atcgccaagt ctgcatatcc ctcggcggcg caagtgctgc
     4321 gaaagatact cgacgcctgt cgggagacga catgatccgc ctggccggcg gacgactcta
     4381 tgacccagtc aatgggatcg acgggatcgt gcgcgatgta tacatggatc gcggccgcat
     4441 tgttcccgcg ccggcggctg gcgggcgcat tgacactgaa tacgacgtgc agggacgcgt
     4501 catcatggca ggcgccatcg atttgcacac gcatatcggc gggggcaaag tcaatatcgc
     4561 gcgcgccatg ctgcccgaag accatcgcaa cagcgccttt gcacgcactg gcatgacgcg
     4621 tgcgggcagc ggcgtggtgg ctccgtcgac atttgcagcc ggctaccgct acgcggaaat
     4681 gggctacaca gcctgcttcg aaccggccat gctgccggtg aatgcgcgcc atgcacacat
     4741 ggaaatggcc gacacgccga tcatcgacaa gggcggctac gctttgctgg gcaatgacga
     4801 ttttctgctg cgcatgctgg ccgcaggggc cgaacagaaa ctcatcaacg actatgtcgc
     4861 ctggaccttg catgccacgc aatgcatcgc aatcaaggtc gtcaaccccg gaggtatcag
     4921 cgcattcaag tttaatgcac gcagcctcga tgtcgacgaa ccgcatccgc actatcgcgt
     4981 ttcaccgcgc gccatcctga ccgcgctgac gcgtgccctc cacgaactgc gcgtaccgca
     5041 tccattgcac gtgcatgcaa gcaatctcgg tgtgccgggc aacgtggcca cgacgctggc
     5101 cacgatggaa gcagccgaag gcctgcccat gcacctgacc cacattcagt ttcatagtta
     5161 tggcaccgaa ggtggacgca aattctcatc ggctgccgcg gacatcgcac aagcactgga
     5221 gcgccatgcc aatgtctccg ccgacgtcgg tcagatcata ttcggccaga cggttacggc
     5281 ttccggcgac accatggcgc aataccgcaa tgccccgcac gcgcacccag ccaagtggct
     5341 ctgcatggat atcgaatgcg atgcgggctg cggcgtcgtg ccatttcgct atcgcgatgc
     5401 caacttcgtc aatgcgctgc aatgggccat cggcctggaa ctattcctgc gcgtgaaaga
     5461 cccctggcgc ctgtttttaa ccacggacca cccgaatggc gggccattca ccagctatcc
     5521 gcatttgatc cggctgctga tggattgtaa ttttcgcaac gagaaacttg cccagatcca
     5581 tcccgaagca gctgcgcata gccaattatc cgggctagac cgggaatact cgctgtacga
     5641 gatcgcgacc atgacgcgcg ccgcgcctgc ccgcagcgtg ggacttgccg accgcgggca
     5701 tctcggaatc ggcgccgccg ccgatgtcac ggtctatcgc gatattgccg atcgcgagca
     5761 aatgtttgcg gcacctcaat acgttttcaa agacggcgag ctggtcgcgc gtgatggcag
     5821 gatcgtcaag gtaacgcgcg gcgcaacgca tgtggtgcgg cccgactttg accggggaat
     5881 cgaaaagcaa ctgcgcgatt atttcgagcg ttaccacacc atccggttcg acaactttcc
     5941 gctttcgagc gaggagctca cagaattcgg cagccgcgag accctggtgc atccgtgttc
     6001 aaggccaatc tcatgaaact gaacggcgtc gcaatagacg ataccttcgc cgaagcgttc
     6061 ggcatgcgcg ctacgcgcat cctcatcaca gccctgaacc tgaagtgggc ctatcacgcg
     6121 gctcattcgc tgaccggatt cgccacctcg gtaatcggct gcggatgtga agccggcata
     6181 gagcgcgaac tactgccgga cgaaacaccc gataatcgtc ccggcatcgc ggtactgctg
     6241 ttcgcggttt cgtcgaagga gcttgccaag cagatcgagc gccgcgtggg ccagtgcgtc
     6301 ttgacctcgc cttcttctgc ggtctatgcg ggactcgagg gtgaacaggt cagcctcgcc
     6361 aaaaatctca ggttcttcgg cgacggtttc cagatgtcca aagtcatcgg aagccagcgt
     6421 tactggcgcg tgccagtcat ggatggcgaa ttcctgggcg aagaaaccac cggcatgatc
     6481 aaagccgtgg gcggcggcaa ttttctcgtg ctggcacgcg gccaatcgga ggcgctcgaa
     6541 gcctgcgagg cggcgattgc cgccatgtcg aaagtgcgcg gagtcatcat gccatttccg
     6601 gggggcattg tgcgctccgg ctccaagatc ggctccaagt acaaggcact ttccgcgtcc
     6661 accaactcgg ccttttgtcc tactttgcgc ggcgcagtga agagtgaatt gacaccggaa
     6721 accggcgccg tactcgaaat agtcatcgac ggactgtcgg aaagcgatgt gcgcaacgca
     6781 atgcgggccg gcatccgtgc ggcgtgctcg ctgggcccca ccggcggcgt gcagcgcatc
     6841 agcgcgggca attacggggg caagctgggc ccattccatt tcagattgca cgacattctg
     6901 caatgacgcc gctgattctc acattgcgcg caacggcgcc tttcgacatc gatctttccg
     6961 ccgtgacgcc tgaccggctg accggcgtca aggtcgcgga cgttgcattg acgggtgggc
     7021 gacagttggg ggagttgttc gatatcacgg gcgaagattc gcaaaacatc atcattcgca
     7081 atcccgatga acgtctgacc catatcggag ctgggatgag ggagggcgtc ctgacggtcg
     7141 agggcaattg cggcgcatat gccggcctcg ccatgcgggg tggcatgctg atcgtcaagg
     7201 gcaatgcagg ggacttcgcc ggtgcggcca tcgccggaag caggcaaggc atgcgcggcg
     7261 gaaccattgc gatttacggc aacgtcggcg accgcgtcgg ggaacgcatg cgccgcggcc
     7321 tggtgctgat cggcggagac gcgggcgcat actgtggcgc gaacatgctc gccggaacga
     7381 ttctggttgc cggtaaggtt ggcctgatgc ctggatttct attgaagcgc ggctcattgt
     7441 tgctcagccg cgcgccaaac ccgctacccg caacgttcca ggacagcgga gaacattcgc
     7501 tgctgttcct gacgctgctg gaaaaacggc tgcaacgcga aggccccacc ttggcgcatt
     7561 ttctgcctgt cgctcggacc gtgcggcgct actgtggtga tatggcctgt ggtgggaccg
     7621 gggagatgct ggtattcatc tgaaccgccc gttactggag gtcttccagt tcagacgttc
     7681 gcgtttcgta agctctcaac atgcactcga cgtcgttaca gacatcccgc acatcatgca
     7741 tccatgaacg ctggctctcc atcaggacct ccggctgtat gccgcgttta attgcagcgc
     7801 ggaaagcaga cgccatgcgc tgatctgccg acgcaagccg cgaatttgag cacaatagct
     7861 tctcgctgtt ggtcttggcg cgactgcaat ccacggcagc gaacaccggc gcagcaaggg
     7921 ccagcgcgag cgtgaaccac aaacaggaca gtttgttcat caaatcgtca gccggccatc
     7981 ctgcagcaac ggcacggcct gaagaatgct caagccggct gcgtagtgca cttcgtgaat
     8041 gagattgcgc tcgatcatca tgcggcccac gcgggcgcgc agcaggcaat cggcggcgtc
     8101 ctcattgcga aacaggctgc gcactgcaag cgcggcgtct gaaaaatcgc tgttttcgtc
     8161 gagcgcgcgc gacagcaagt ccaccagata acccgcgccg tacagatctt cgaggttgac
     8221 catgcccatc gacccggagc agacaatcag gatggtcttg cccgggtgct tgcccaatac
     8281 caggtcgacc actgcttcgc cattgacgag tgctgccgca tagacatggt cggcccgcga
     8341 cgattgtcgc aaagcgacag tgccgtttgt cgttgagtaa atcaatttgc gacccgctat
     8401 tttttcttcc atcaaggcca gcggcgttgg cgatgcaaaa ccaggcagcg tttcagcata
     8461 gagctcgccg gcaaggatgt aactgccttg cggatgcttc tgcgcttccg cgcgcgcgcc
     8521 ggcctcatcc aaagtcggaa tgacttcggt ggcgccattg gcgagcgccg caatgatggt
     8581 ggaggtcgca aacaatacgt ccagcacaat ggcgaccttg ccttcgaggc gctcatggtc
     8641 caactcctcc ttgcggtaaa gcacatgaac cttgtgcttg aacagcatgg tcacttgtcg
     8701 gcactccccg gactgcggcg gcgaaacatc ccataaccct gcatggacat caccagctct
     8761 ccctcctggt taaacacttc ccaaagatta ttgacgatgc cgatatcggg cttgctcttc
     8821 gaggcgcgcg tttcgagcgt ggtgcgccgt gcgctgataa tgtcgcccgg tcgcaccggc
     8881 tttagccatt tgagatggtc gataccggga gagccaacac tggcggcttg cgcaaggtat
     8941 gcatcgcaca tcatgcgcat gaccatggcg caagtatgcc acccactggc aattaacccg
     9001 ccatacatcg accgcttcgc agcagcctcg tcgatgtgga acggttgcgg atcaaactgc
     9061 gttgcaaacg caatgacttc ggccttgtcg acaagcctgc ggccaatctg ctcgacttcc
     9121 ccgacgtgga aatcttccca ataccttgtt gtcaacttca gcccctaaag gaagttcagc
     9181 gccattggcc tcgagtgttc ggcttgtcat ggccgcaatc ccgtttcaaa ttttgaccca
     9241 tgatttgcgc gggggctgcg tttgcgcact ggtctcaccg tgcttcatcg ttgcaaaacg
     9301 ggtgcgctgg ctggcagcgt tgccgaatag cacatcgatt gcagtcagcc gtttgaaata
     9361 gtggcttaca ttcaactcat ccgtcatgcc catgccgcca tgcagctgga tcgcctgctg
     9421 gccgaggaac cgacccgcgc ggccggtcag ttctttggcc gccgccacag tgcggcgccg
     9481 ttcaccaata tcattcgagt cgacttttga tgccgccagc aaagccattg aacgcgcttg
     9541 ctccaattgc accagcatat cgaccgcgcg atgctgcaag gcctggaaac ggccaatcgg
     9601 ctgaccgaac tgctggcgcg ttttgagata ctccaccgtg gccgcgtgca acgcgtccat
     9661 gatgccaact gactccgcgc ataaggctgc catcgcacgg tccagcgcaa cgtcgatcag
     9721 cggcgaagcc tgatccagcg tgccgatgac cgattcggga ctcagtttta cgccggagag
     9781 ctgcaggtca agtgcgcgcg taccatcaaa caatggatat tcctgaccgc cgaggttttc
     9841 ttcgtccgcc tcgacgatga acagcgtgac cccgcgcgga tccgcatcat tgccgctggt
     9901 acgcgccgac acgataaatt tgtctgccac gctgccgtgc agaaccaggg atttttcgcc
     9961 ttccagaacg aaattgttac cgtcccgggt ggcgcgcgtc ttgacctggc caagatcgaa
    10021 acgcgcgtcc ggctcggtgt gtgcgaaggc catgatcatc gacccatcga tgatcttcgg
    10081 cagcagaatt tttttttgcc actccgctgc acccaacgcc acgcagctgc cggcgagtac
    10141 gacggtagca aggtaaggct ccagcaccag attgcgcccc aacgcctgca tgaccagcat
    10201 ggtttccacg ccgccgccgc cgaagcctcc gtcgtccggc aagaatggaa cactcagcac
    10261 gccgagttcg gccaggcctt gccagagttg tgcattcatc ggctggcgat cggccagcat
    10321 ctggcggcgc tgctcgaagc tgaaattttt tgccaggaag cgatccacgc tttccttcag
    10381 cattgcttgt tcttcggtca gattaaaatc catagtcgta aaccttgaat cacggagctt
    10441 gcgaggcgca cggagaactg caattccttg gcagacttcc ggcgctcatc ctgcgtttgc
    10501 tgcgtgttct gcgtttcctc cttcgtggtg acgcttccgc cgctcaaagt ccgagcacca
    10561 tttgcgcaat gatattgcgc tggatttcat tggagcctcc gtaaatcgtg gtcttgcggt
    10621 agttgaagta gcgcgcggcc agcggggccg cgtagtccgg accgacatgc gcgccgatcc
    10681 agtgatcgcc ccatgccatc ggcagatgcg gcagcgcgtg cgggccgacc gcgtccatca
    10741 tgagttcggt gagttcttgc tggatttcag tgcctttgat tttcaggatg gatgcttccg
    10801 ggcccggggc cttgccgtcg cgatccgcgc ttgctacccg caggcaggtt atctcgagtg
    10861 ccatcaagtc aatctccacg cgcgcgacac gatcgcggaa cagcggatca tccatcagcg
    10921 gcctgccgcc ttcctgctct tgtgtggcga tatgcttcag atagtgcaac tcgcgttttg
    10981 atgctccgac gccggcaatg ccggtgcgct catgcgacag caggaacttg gcgcacgtcc
    11041 agccccggcc ttcttcaccg accaggttct gtaccggcac ttgcacgttc tcgaaccaga
    11101 tctcgttgac ttcatgttcg ccatcgagcg tagtgatcgg ccgcacggtt acgccgggcg
    11161 aatgcatgtc gatcagcagg aacgaaatgc cgtcctgctt cttgctttcg ttggacgtgc
    11221 gcaccaggca gaaacacatg tcggcccatt gtccgtgcgt gttccaggtt ttctgtccgt
    11281 tgacaatgta tttgtcgcct tcgcgctgcg cgcgcgtctt gagcgacgcc agatccgaac
    11341 ctgagcccgg ctccgaatag ccctggcacc accagtcctg gctcgacagg atgcgtggca
    11401 ggtaatgctg cttctgctgg tcggtcccga atgcaatgat gaccggacca accatgtgca
    11461 acccgaacgg cgttaatcgc ggcgcgcctg ctgccgcgct ctcgtcttcg aaaatgaagc
    11521 gctggatggc agaccatccg gtgccgccgt acgccacggg ccaacctgca gcaatccagc
    11581 ccttctcgaa cagaattttt tgccagcgta cgtaatcttc tttgccaaga cgcttgtgct
    11641 ccagcacttt ccgggacagg tcacccggca ggtggccgcg gatgaaatcg cgcacttcgg
    11701 cgcggaatgc cgtttcttct gtcgtgtagt tcagatccat aagcattacc cggggagttg
    11761 tgacggacgg ccttggaatc gcccggcaac gaaggataag cgatgctcag gggtcaggca
    11821 aatccgtgct ggaacgcatg cgccacgccg cactataata aacgcccaac tcaaggctct
    11881 tgcgcggccc gttccggggc tcaccgccaa ggaggagatc atgaacatgc cagacgaaaa
    11941 agctctgcgt catttgaata cctcgctgaa tctggatccg ctgtggattc ccttcaccgc
    12001 aaatcggcag ttcaaggctg cgccacgttt gttggtttcg gccaaagaca tgcattacca
    12061 aacccatgac ggccgcaaga ttttggacgg cattgcgggt ctgtgggctg tcaacgccgg
    12121 ccattgtcgg cgcccgatcg tggacgcgat caagcagcag gcggaaacca tcgattatgc
    12181 gaccggtttc cagatgcacc acccggcggc attccaggcg gctgaaaaac tcgtggccat
    12241 cacgccgccg ggcctcaacc gggtgttctt ctgcaactcg ggatccgaag cggtcgacac
    12301 ggcgttgaaa gttgcactcg cctatcatcg cgtgcggggc gaaggaaccc ggcgcatcct
    12361 gattggccgc gaacgcggct atcacggtgt cggctttggc ggcatttcgg ttggcggcat
    12421 ggtgggtaat cgcaagacgt tcggcaacat gctgggatca gtggatcatc tgcgccatac
    12481 gcacgacccc gcgcgcaacg ctttctcgcg cggactgccc gagcacggcg cggaattggc
    12541 cgatgatctg gaacggctgg tacaactgca tgatgcgtcc aatatcgcgg cagtcattat
    12601 cgagccggtc gcgggttcca ccggcgtact gctaccgccg aaaggttacc tgcagcggct
    12661 gcgcagcatt tgtgacaaat acggcatcct gctgatattc gatgaagtta ttaccgggtt
    12721 tgggcgcacc ggcaaggcat tcgcggcgca gaccttcggc gtgacgccgg acctcatcac
    12781 ctgtgccaaa gcgctgacca acgccgcggt ccccatgggc ggcgtgatcg tgaagcaagg
    12841 catttacgac acattcatgg aaaaaggcgg gcctgattcg gccatcgaat ttttccacgg
    12901 ctatacctat tctggtcatc cgcttgctgc tgccgcggct tgcgcttccc tcgatttgtt
    12961 caaggcggaa aaactgttcg agcgcgtcgc cgaactcgca ccgtactggg aagacgcggc
    13021 acattcgctc aaaggtctac cgcacgtgat cgatatccgt acgatcggtc ttgtggccgg
    13081 gatagaactg cagccgatgg ccgaaaaacc gggggcacgg gcgttcgacg cgttcttgcg
    13141 cgcctatgac aagaatgtgc tgatccgcac caccggcgat acgattgcgc tgtctccgcc
    13201 gttcatcatc gacaaggcgc aaatcgatca actattccag actgtacgcg atgtgctgaa
    13261 ggcgctgcct taaggcgatg cggctacagc atatccctga gaattgaagc caggattcgc
    13321 aacggaatga aactcgtcat cgcacagatg cagcacgaga ccaacacgtt ctcgccggtc
    13381 gccacgccgt gggaagcatt cggcaacaac ggcccgctgc tcggcgaggc ggccggccaa
    13441 gccgtgcacg ggaccaactt tgcgatggcc gccttccttg atttggcgcg agaggcgggt
    13501 gcgcaggtcg tcacgccggt cgccgcgtgg gccaatccaa gcgggcccgt ggatggcgcc
    13561 gcctacgatc gcatttgcgg tgcaatctgc gaagcagtgg cccagggttg cgacgccgtg
    13621 ctgctggacc tgcatggcgc catggtggtt gcagatcgca ccgacgacgg cgaaggcacg
    13681 ctgctggaga aagtgcgcgc tttggcgcca cgcacgccga tcgcagtgtc actggacctg
    13741 catgccaacg tcacggaacg catggtgcgc aattgcgacg tcattaccgg ctataaaact
    13801 tatccgcacg tggatcagta ccaatcggga gaactggccg gctcgctgtt gctgcgcatg
    13861 atgcgaggtg agatcaaacc agtgatggct tggggtaatg cgccggtcct cgcgcagacg
    13921 ctgcgccaga acacagcgga aggcgcgatg aaggattttg tcgaggccgc tcgcgccgcg
    13981 gagcgcaacg gactgcttgc ggcaacggct ttcggcggct tccagatggc agacattcac
    14041 gatgcgggat tgagcgtggt aaccgtctcc gacgcaaacc gggcggcggc cgagacggcc
    14101 tgcaatgcga tcctcgatgt tgcctgggca caaaaggagg atttcatcta ccgcgggcaa
    14161 ccgttgcagg aagcgattgc gcaagccagg cgcatggggg aacaatccgg cgggccgatt
    14221 ttgttgctgg accatgccga caactgtgct tcaggttcca cgcaggacac gatgtatgta
    14281 ctcaaagagg caatgcgcca ggggttatcc ggaatagccg tggcgccgat ccgcgacccg
    14341 gaggcggttg gaaaaatgat caatgcaggg atcggcgcgc gcgttacgct tgccgtcggc
    14401 ggcaaaatgg acatgccgtc catccggcgc aaaggggagc cgcttacgtt gaccggggtg
    14461 attcgggcca tcaccgacgg cgaatacacc atcaccggcc ctcagttcac cggcatgcgc
    14521 tgtttcatgg gcaagactgc cgtgctggac accggcgtgg cgcaaataat cgtgagcgaa
    14581 cgtaaccagg aaccctggga ccgtggcgta tacgagagcg tcggcattga cccggcacgc
    14641 cagcgctatc tgctgctgaa atcgcgcatg tactttcgtc ccgtgttcct gccaatcgcc
    14701 aaaggcatgg tgttctgtga cgcgcccggt gtgtcgtcgt cggactggac cgaattcgaa
    14761 tatcgcaagc tgcggcgacc gatctacccg ctggatgaac atttcgccaa cggccgcaac
    14821 gccatggccg cgtaaccgat aacaggagaa gcaacgtgaa caagccattg aaccaggcag
    14881 ccgtcctgga agaacttcac cactacatcg gcggcgctga agtcgccggt cgcagcggtc
    14941 actacggcga catctacaac ccggcgctgg gcagcgtcaa agcgcgtgtg ccgttcgcca
    15001 ccgcagctga agtatccgcc gcggtcgacg cggcgcagaa agcgtttcct gcctgggccg
    15061 ccacaccgcc gctgcgccgc gcgcgtgtga
//
LOCUS       DATMZQ010000221         3058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3968031, whole genome shotgun sequence.
ACCESSION   DATMZQ010000221 DATMZQ010000000
VERSION     DATMZQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3968031"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VN496_07230"
     CDS             complement(<1..591)
                     /locus_tag="VN496_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16780.1"
                     /translation="MNHYPVQASIKSTCVCLAIAGALNGAPACAADWGIDISAGLSSD
                     DNVTNAFEAADRKADTTAILDLAAGLHHEIRPGTSFGLNFISESATYMSYSGLTNLGL
                     GVRGQLRKKFGLGAAAPWTALSVAALHRDYHDDNRDGWQYDASLAVGKRLDERWSLHA
                     MAHYDRYAADEHQPQVIPGLSSAAYDVSGRSIGAGVS"
     gene            complement(588..2975)
                     /locus_tag="VN496_07235"
     CDS             complement(588..2975)
                     /locus_tag="VN496_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF16781.1"
                     /translation="MKLRSLSTRMALVFTIMFIVVQAAVLVLVDTVSSKIARDRNTEE
                     LHIGARVLERLLDQNRQSLLQAAEVLSRDFAFRKAIATGDSATILSALNNHGSRIGAT
                     VMMLASIDGHRVADSLHPGAASQPFPWPALVRTAQDQGKASSILPIDGALYHIVVVPV
                     LAPDPIAWVAMGFSVNEAFVNDLLMLTSLHVSFLKTSASGRWMVLATTRKDEALERSL
                     AVLPPVLGNAPEPMRLGDYDTLITSRVQQSGDPLTIALQRSGREGLEPITQLKSLLAL
                     LTAASIAAFIFGSVVLARRITQPLAQLSTFSQRARDGHYSDRLQLKRSDEIGALSANF
                     NHMLEGVAAREAEIMRLAYVDTLTGLPNRAMFNRRLTEALKQFRETGTPACVLIMDLD
                     RFKVINNTLGHEAGNKVLQAVAARLRESVRELDTVCRLGGDEFGILISGADAKRGQVL
                     GRMIQAVLEEPIDLDGQPVDVGSSIGVAQCPVHGKDAGLLMRHADIAMYAAKHAKSGV
                     AIYEPQLQNSHSEQLHLVGDLRKAIVENQLELHYQPKLDLRSSQILGVEALVRWCHPE
                     RGMIAPAEFVLFAEQTGAIRQITRWVIGEAMQQCRQWIASGLSLGVSINVSTRDLLDR
                     ELPQVFAAAAQKNRIPAELITVEVTESALIEDPNRAQETIRALKDFGARISIDDYGTG
                     YSSLAYIQRLQCDELKVDRAFVTHAHEQRKDLAIVRSTVELAHSLGLTVVAEGVETPE
                     AMKILRELGCDVAQGYWLSRPQPPDGLRDWLASCGWSTSTSHGEPQRRADRLHAV"
BASE COUNT          601 a          929 c          907 g          621 t
ORIGIN      
        1 agatacgccg gcgccgatgc tacgtccgga cacgtcgtag gctgcgctcg acaaacctgg
       61 aatgacctgc ggctgatgct catctgctgc gtagcgatca tagtgcgcca tcgcgtgcaa
      121 gctccagcgc tcgtcaagcc gcttgcctac ggcaaggctg gcatcgtact gccaaccatc
      181 gcgattatca tcgtgatagt cccggtgcag cgcggccacc gagagcgcag tccagggtgc
      241 tgcagcgccg agcccgaatt ttttccgcaa ttgcccgcgc acaccgagtc ccaggttggt
      301 gagaccggaa tagctcatgt aggtggctga ctcggagatg aagttcagcc cgaagctggt
      361 cccgggccga atttcatgat gcagcccagc tgcgaggtcc agtattgccg tggtgtctgc
      421 tttgcgatcg gctgcctcaa acgcgttggt gacattgtcg tcactcgaca ggccggccga
      481 aatatcgatg ccccagtcgg ccgcacacgc cggcgcgcca ttcagcgcgc ccgcgattgc
      541 gagacacacg caagttgatt tgatgcttgc ttgcacaggg tagtggttca caccgcgtgc
      601 aggcggtcgg cgcgtctttg cggctcgccg tgcgaggttg aggtggacca gccgcagctt
      661 gcgagccagt cgcgcagccc gtccggcggt tgcggccggg aaagccagta accttgcgcc
      721 acatcgcatc ccagctcgcg caggatcttc atggcttccg gtgtttctac gccttcggcc
      781 accacggtca agcccaggct gtgggccaat tccacggtcg agcgcacgat ggccagatcc
      841 tttcgctgtt catgcgcatg cgtgacgaat gcgcgatcca ccttgagttc atcgcattgc
      901 aggcgctgga tataagccag cgacgaataa ccggtgccat agtcgtcaat cgaaatgcgc
      961 gccccgaaat ccttgagtgc gcggatggtt tcttgcgcgc ggttcggatc ttcaatcagc
     1021 gcgctctcgg tcacttcaac cgtaatgagt tcagccggaa tccggttctt ctgggcggcg
     1081 gccgcgaaca cttgcggtag ctcgcggtcc agcaggtcgc gtgtggatac gttaatggac
     1141 acgccaagtg acaagccgct tgcaatccat tgccgacact gctgcatggc ctcgccgatg
     1201 acccaccgcg ttatctggcg gatggctcct gtctgctcgg cgaacagtac gaattcggct
     1261 ggtgcaatca tgccgcgttc gggatgacac cagcgtacaa gtgcttccac gccaaggatc
     1321 tgtgagctgc gcaggtcaag cttcggctgg taatgcagct ccaactgatt ttcgacgatg
     1381 gccttgcgca aatcgcccac caggtgcaac tgctcggaat ggctgttttg caactgcggt
     1441 tcgtagatcg caacgccgga tttggcgtgc ttcgcggcat acatcgcgat gtccgcgtga
     1501 cgcatcagca ggccggcgtc ctttccgtgc accgggcact gcgcgacgcc aatgctcgag
     1561 ccgacatcaa ccggttggcc atccagatcg attggttctt ccagcaccgc ctggatcatg
     1621 cggccgagca cctggccgcg ctttgcatcc gcgccagaga tcagaatgcc aaattcatct
     1681 ccgccgagcc ggcagacggt atccaattca cgcacggatt cgcgcaatct cgctgcaacg
     1741 gcttgcagta ccttgtttcc cgcttcatgc ccgagggtgt tattgatcac cttgaaccga
     1801 tcgagatcca tgatcagtac gcaagcgggt gtccccgttt cacggaactg cttgagcgct
     1861 tcggtcagcc ggcggttgaa catggcgcgg ttcggcaaac cggtgagggt atcgacgtat
     1921 gcaaggcgca ttatctcggc ctcgcgcgcc gcaacacctt caagcatgtg gttgaagttt
     1981 gccgacagcg cgccgatttc gtcgctgcgc ttcagttgca atcggtcgga atagtggcca
     2041 tcgcgtgcac gctgcgagaa tgtcgagagt tgtgcgagcg gctgggtgat gcgacgcgcc
     2101 agcacaacgc tgccgaagat gaacgcagca atactggccg ctgtcagcag cgcgagcaaa
     2161 gacttcagct gcgtaatggg ctcgaggcct tcgcggccgg agcgctgaag cgcgattgtc
     2221 agcggatcgc ccgactgctg aacgcggctg gtgatcaaag tgtcatagtc gccaagacgc
     2281 atgggttccg gcgcattgcc caacaccggt ggcaagacag ccagcgaccg ttcaagcgct
     2341 tcgtccttgc gagtcgtggc tagaaccatc cagcggccgc tggcgctggt cttgaggaaa
     2401 gagacatgta gcgaagtcag catgagcaga tcgttgacga acgcctcatt caccgagaat
     2461 cccatcgcga cccatgcaat tggatccggg gcgagcacgg gcaccaccac gatgtgatac
     2521 agggctccat cgattggcaa gatcgaagat gcctttccct gatcttgcgc cgtacgcacg
     2581 agagccggcc acgggaacgg ttggctggcg gcacctgggt gcagtgagtc agccacccga
     2641 tggccatcga tcgatgccag catcattacc gtcgcaccaa tgcggctgcc atggttattc
     2701 agggccgaca ggatggttgc ggaatccccc gtggcaattg ctttcctgaa ggcgaaatcg
     2761 cgcgacagaa cttctgccgc ttgcagcaaa ctctgacgat tttggtccag cagacgttcc
     2821 agcacgcgcg cgccaatgtg caattcctca gtattgcgat cacgcgcaat cttcgagctg
     2881 acagtatcga ccagcactag tacggcagcc tgcaccacga taaacattat cgtgaagacc
     2941 agggccatgc gcgtgctcag gctgcgtaac ttcatcccct acctctgcta tttataggac
     3001 cctgctgggc ccggcgggga attgtcagac cttctcacag ctcgaatgcg gatgacga
//
LOCUS       DATMZQ010000222         8443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3969307, whole genome shotgun sequence.
ACCESSION   DATMZQ010000222 DATMZQ010000000
VERSION     DATMZQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3969307"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1091
                     /locus_tag="VN496_07240"
     CDS             177..1091
                     /locus_tag="VN496_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF16782.1"
                     /translation="MPTQYHTLPRSMWVLLALLTFGWGLNWPMMKMTLAELPVWTFRG
                     LCVAAGAAGLFILARLGKQSIRVPPEHWLPLIVISICNVTLWNVLIGYGLQMLPAGRS
                     AILAYSMPVWAVLLSVFILHEKLTRRRIAGLMLGMAGMAILLGGEMSVMRASPLGALC
                     VLGAAIGWALGTVLMRRYPMKLSTTALTAWQLLVGGLPIMVGAFLIDWGNWRPIGLGA
                     TIGLVYNLTFVFIFCYWAWFKIATTAPPSVSSLSTLMIPVVGVFSGIWVLGESPEWRE
                     YVALLLVIAALATVLIPPRMTRQAAKRP"
     gene            1178..1783
                     /locus_tag="VN496_07245"
     CDS             1178..1783
                     /locus_tag="VN496_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXF16783.1"
                     /translation="MKTSLLLLLVWAISAGIAHADIYRYVDEDGTVHFTNVPQDSRYK
                     VYLKETRKPDPVTDTLASEIHHYDAKDRARYARTIQDAAKATQLDAALIHAVISAESG
                     YNPFARSRKGAAGLMQLMPETAKRYGVKNRLDPTQNISGGARYLRDLIRMFNNDLQLA
                     VAAYNAGENAVIRAGNRIPPYQETMTYVPRVMSYYRKYRTS"
     gene            2030..3127
                     /locus_tag="VN496_07250"
     CDS             2030..3127
                     /locus_tag="VN496_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXF16784.1"
                     /translation="MKIETIETMVLRIPYTSGGPSDTEVWGGQAWQTADVLLVKISTD
                     AGVTGWGEAFGYNVIPATKAAIDQILAPMCIGRDPHQIESLMLELQQKLHIFGRSGPI
                     IFGLSGIDIALWDIAGKLAQQPVHQLLGGGKPKLDCYASLIRYTAPKLVAANVERALS
                     DGFRHIKLHEIELAPVRAARQSTGENVDIMLDVNCPWTLREALDMSQKLAPLNLRWLE
                     EPVWPPEDHAALAKVRADGGIPVAAGENATTIAQFKHLFDAGAVDFVQPSPAKMGGVS
                     ELRKVFTLADAYNVTVMPHSFYDGPAFLAGVHVNAALGRGSMVEWRYFDLERRLYGER
                     AVPRNGSIDVPQSPGLGYDPDPEVVKRYRAD"
     gene            3178..3702
                     /locus_tag="VN496_07255"
     CDS             3178..3702
                     /locus_tag="VN496_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16785.1"
                     /translation="MNLERITVEFHAEEDRLMVRVFFDKKAEIQFWLTRRLVKRIWPV
                     LLQMAQSRPDIQLQPNPEVRKALLGMQHEKALQEVKFTNAAQEAQREHPLGHAPMLVS
                     KVRARRNAKGQTALSLQPQQGTGVDLALGDTLIHGLMKLMQDTVVKAEWDLDLAVPTF
                     AYAAYEKDTNRTVN"
     gene            3740..5224
                     /locus_tag="VN496_07260"
     CDS             3740..5224
                     /locus_tag="VN496_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16786.1"
                     /translation="MSNFATIFLRTRLAPAILLLAMLPACATFRSYDSELTRTINLVS
                     AGNVDGAIKQLDSNNKRSDKDLLYYLELGELQRLNHRYDESEKAWLAADGHVQAWENT
                     AMANPEKVLGSITSVLVNDKTIPYEGHDYEKVMLTTRLALDRLARGDFDMARVDIKRT
                     HEREAIIAELREKELKKTEDEARKHGVRSGFKELNGYPVQTIDNPEINALKNSYENAF
                     SHYLAGFVYEALGEPSLAAAGYRHAIELQPGRPLLEDALAGLDTRVMVRDDGYTDVLF
                     VLESGVAPARKSRQFNLPIPYRDRLLFVPVSFPVIAPTQPAFMPSELQITGEAAITTT
                     LLTSVDLMARKALQEEMPGIMLRGMIRSTSKAAAQYQASKNSNQSGLVALAGLAVTIG
                     TVVTESADERGWRSLPGQIAIARLRIPSGEHTLAFGANPGGQGLRFSVSGRYAVISLR
                     LIGNAAFLIPSPVVPSEKSGTQASNPLPLVVQSTTTASKTFGQP"
     gene            5244..5681
                     /locus_tag="VN496_07265"
     CDS             5244..5681
                     /locus_tag="VN496_07265"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HXF16787.1"
                     /translation="MKLTVLAAILTASIIVAGCGGNKFERPGYAYGCPTGDDKKIKSK
                     EDLIKAKLEVAGSLPDIDVVDLRCSDRSALLRVDMDLKNDSKEVRRIAYRFRWLDKEG
                     MRAWDDESWKPLLIYGKTLYTVTTMSPSQEATDFRVVVMDQDK"
     gene            5705..6298
                     /gene="lpoB"
                     /locus_tag="VN496_07270"
     CDS             5705..6298
                     /gene="lpoB"
                     /locus_tag="VN496_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HXF16788.1"
                     /translation="MMTQSLPVRLASIFAFCVAVGLSGCASEKTKYGDARGVETVTNQ
                     FGSTDLQMIAESMTRSMVNAPVVAAGNLPIMTVQEVKNKTSEYIDTRAITDSIRVELQ
                     KSGRVRFAADAAAMDQQVEELKRQQSDYYDPKKSSEIGKMVGAAYRLEGNISSIVKEA
                     KGVKDIYYKFNLQLWNIQNGLLEWTDEKEIRKTTSKT"
     gene            6591..7391
                     /gene="phnC"
                     /locus_tag="VN496_07275"
     CDS             6591..7391
                     /gene="phnC"
                     /locus_tag="VN496_07275"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HXF16789.1"
                     /translation="MIEVRGLTMRYGELAALTDVSLDVAAGEILVVLGPSGAGKSTLL
                     RCINRLIEPSAGEVRMAGVPTPGDRTGLRRLRSQIGMIFQDHNLVPNLSVLKNVLTGR
                     LSHMPTWLSVLQLFTDADVDIALECLRRVDLEARAWNRANRLSGGQQQRVGIARALAQ
                     RPRAILADEPVASLDPQSARIVLDDLHRAARDLSIAVLCNLHQVEYARDFADRIVGIS
                     AGRVVFEGTPAQLDNAALARIYPGLNSTSGGESSSRRSADVERSTAVQ"
     gene            7466..8200
                     /gene="phnE"
                     /locus_tag="VN496_07280"
                     /pseudo
     CDS             7466..8200
                     /gene="phnE"
                     /locus_tag="VN496_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
BASE COUNT         1877 a         2349 c         2441 g         1776 t
ORIGIN      
        1 ccagaacagc acgaagcgca cgagcggata attcagcatg gcgacgatgt tagcacgcac
       61 cgcatttccg aggtgtcgcg gggtgctgat tcgctcactg ttacatacgc gttgagtccg
      121 ggcaaatctg cgctgtttgc cttgctgtac acttcgcgat ttttcgagaa tccttcatgc
      181 ccacgcaata tcacaccttg ccgcgttcaa tgtgggtgct gctcgcttta ctgacgtttg
      241 gctggggatt gaactggcca atgatgaaaa tgacgttggc cgagttgccg gtctggacat
      301 ttcggggact ttgcgtggcc gctggggcgg caggactgtt cattctcgcg cgtctgggca
      361 aacagtcgat tcgcgtgccg ccggaacact ggcttccgct catcgtgatc tcgatctgca
      421 acgtcacact gtggaacgta ctgatcggct acggacttca gatgttgcca gccggacgca
      481 gtgcaattct tgcctacagc atgccggtct gggcagtact gctgtctgtt ttcatcctgc
      541 acgaaaagct cactcgccgg cgcatcgcgg gattgatgct tggcatggcg ggaatggcca
      601 tcttgctggg cggagaaatg agcgtcatgc gcgcctcgcc gctcggcgca ttatgcgtgc
      661 tcggggctgc tataggctgg gcgctcggca cggttctcat gcgccgctat cccatgaagc
      721 tcagtaccac cgcgctgact gcatggcaat tgctggtggg tggtctgcca atcatggtgg
      781 gcgcttttct gatcgactgg ggcaactggc ggccaatcgg cctcggcgcg acgatagggc
      841 tggtctacaa cctgacattc gtcttcattt tttgctattg ggcctggttc aagattgcga
      901 ccaccgcacc gcccagtgta tcctcactga gcacgctgat gattccggtc gtcggcgtat
      961 tcagtggaat atgggtgctg ggcgaatcgc cagagtggcg tgaatacgtc gcgttgctgt
     1021 tggtcattgc cgcgctggca accgttctga ttcctccacg aatgaccaga caagcagcta
     1081 aacgaccatg acatgcatct cctgcggacg tgggccgaac cggccatggg cgctgtagca
     1141 cacgatggct tgaaagttgc acaatacggt tcagtctatg aaaaccagcc tgctactgtt
     1201 acttgtttgg gccatttctg ccggaattgc gcatgccgac atctatcgct atgtggacga
     1261 ggacggcacg gtgcatttca ccaatgtgcc ccaggatagc cgctataagg tgtacctgaa
     1321 ggaaacgcgc aagccagacc cggtcaccga tacgttggcc agcgagattc atcactacga
     1381 tgcgaaagac cgggcacgct acgccagaac catccaggac gctgcaaaag caacgcaact
     1441 cgatgcggcc ctgattcacg cagttatctc ggccgaatca ggctataacc cgtttgcgcg
     1501 ttcccgcaag ggagcggccg gactcatgca attgatgcca gaaacggcca aacgctacgg
     1561 cgtcaaaaat cgccttgatc cgacgcaaaa cataagcggc ggcgcacgct acttgcgcga
     1621 cctgatccgc atgttcaaca acgatcttca actcgcggta gccgcataca atgccggcga
     1681 gaacgcggta atcagggctg ggaatcgaat tccgccttat caggaaacca tgacctatgt
     1741 tccccgggtc atgtcgtatt atcgcaagta ccggacctcg taagacctgc tggccatagc
     1801 agcgatttcg ctttgccgcg gcacctgcaa ttgattgcac cgttgctagg gcttgggcag
     1861 cacatccaaa tgcatgttct ctatacttga taacctcgcc cgcttttctg gtgaattgcg
     1921 ccgtacgggt gcaaggcaat aataatcaaa ggcgagaaat tcgtattggc agccgctgcc
     1981 gtgcttcccc tatcatgcgc aatgttttga ctgtgcgcgt aaatcaacga tgaaaatcga
     2041 aacaatagaa accatggtgc tgcgcattcc ctataccagc ggtggtccat cggacaccga
     2101 agtctggggc ggacaagcct ggcagaccgc cgatgtgctg ctggtcaaaa taagcaccga
     2161 cgccggcgtc accggttggg gcgaagcttt cggttataac gtcatcccgg caaccaaagc
     2221 ggctatcgat cagatccttg caccaatgtg catcgggcgc gacccgcatc agatcgaatc
     2281 tctgatgctt gaactccagc agaagctgca catctttggg cgcagcgggc cgatcatctt
     2341 tggtctgtcc ggaattgata tcgcattgtg ggatattgcg ggaaagcttg cgcaacaacc
     2401 cgtgcatcaa ttgcttggcg gtggcaagcc aaaactggac tgctatgcca gtctgattcg
     2461 ctataccgcg ccgaaacttg tggcggcaaa cgttgaacgc gcgctaagcg acggtttccg
     2521 ccacatcaaa ttgcacgaga tcgaactggc tcccgttcgc gcggcccgac agagtaccgg
     2581 cgaaaacgtt gacatcatgc ttgacgtcaa ctgtccctgg acactgcggg aagcactgga
     2641 catgtcgcaa aaacttgctc cgctcaattt gcgctggctg gaggaaccgg tgtggccacc
     2701 cgaggaccat gccgcgctcg caaaggtacg cgccgacgga ggcattccgg tagcggctgg
     2761 cgaaaatgca acgacaatcg cgcagttcaa acacctgttt gatgcaggtg cggtcgattt
     2821 cgtgcagccc agtccggcca agatgggggg cgtatcggaa ctgcggaaag ttttcacgct
     2881 ggcggacgcg tataacgtca cggtgatgcc acacagtttc tacgacgggc cggcctttct
     2941 ggccggcgtg cacgtcaatg cagcgctcgg acgaggttcg atggtcgagt ggcgctattt
     3001 cgatctggag agacggctgt acggtgagcg agccgtacca cgcaacggat cgatcgacgt
     3061 accacaatct cccgggctgg gctacgaccc cgatcccgaa gtcgtcaagc gctatcgcgc
     3121 cgactgactg aattccggga ccgtttacca aaagcaggtt agcgtaatat tccgctcatg
     3181 aacctggaac gcattactgt tgaattccac gccgaagaag atcgcctgat ggtgcgcgtt
     3241 ttcttcgata aaaaagccga aattcaattc tggcttacac ggcgtttggt caaacgcatc
     3301 tggcccgtgc ttctgcagat ggcgcagtcc cggccagaca tccagttgca gcccaatcct
     3361 gaagtgcgca aggcccttct gggaatgcag cacgagaagg cgctgcagga agtaaaattt
     3421 accaacgctg cacaggaagc acaacgcgaa catccgttgg gtcacgcgcc gatgctggtg
     3481 agcaaggtgc gcgcacgcag aaatgcaaaa ggtcagactg ccctctctct gcagccacag
     3541 caaggcaccg gggtcgacct tgccctggga gacacgctca tacacgggct catgaagctg
     3601 atgcaggaca ctgtggtcaa ggcagaatgg gaccttgatc tggccgtgcc gacatttgcg
     3661 tacgcagcct acgagaaaga taccaatcgc accgtcaact gacgttgcgt tgacgcgttg
     3721 ttgcgacagg acgaacgtct tgagcaactt tgcaacaatt ttcctgcgca cacgacttgc
     3781 gccagcaatt ctgctactgg caatgctacc cgcctgcgcg acattccgaa gttacgattc
     3841 tgaattaacg cgcaccatca atctggtgtc cgcgggcaat gtcgatgggg caatcaagca
     3901 actggacagc aacaacaagc ggtccgacaa ggacttgctt tactacctgg aattgggaga
     3961 actgcagcga ctgaatcatc gatacgacga aagcgagaaa gcctggctcg ctgcagatgg
     4021 tcacgtgcag gcgtgggaaa acacggccat ggcaaatccc gaaaaggtgc ttggcagcat
     4081 caccagtgtc ctggtgaacg acaagactat tccctacgag ggccatgact acgaaaaggt
     4141 catgctcacc acgcgacttg cgcttgaccg gctggcgcgt ggtgatttcg acatggcgcg
     4201 cgtagacatc aaaagaacgc acgaacgcga agcgatcatc gcggaactca gggaaaaaga
     4261 actgaagaaa accgaagacg aggccagaaa acatggcgtc aggagcgggt tcaaggaatt
     4321 gaacggctat ccggtacaaa ccatagacaa tcctgagatc aacgctctca agaacagtta
     4381 tgaaaacgcg ttcagccatt accttgccgg atttgtttac gaggccctgg gcgaaccgag
     4441 tcttgcggcg gcgggatatc gccatgcaat cgaactgcag ccggggagac cgttactgga
     4501 agatgcgctg gccggcctcg atacgcgtgt tatggtccgc gacgacggtt atacggatgt
     4561 gctgttcgtg cttgaatcgg gcgtcgcacc agcacgtaaa tcgagacaat tcaacttgcc
     4621 cattccgtac cgggaccgct tgctgttcgt gccggtgtcc tttccggtta tcgcaccgac
     4681 gcagccggcg ttcatgccat cggaattgca gattacgggc gaggcggcga tcaccacaac
     4741 gctcctcacc agtgtggacc tgatggcgcg caaggcactt caggaagaaa tgccgggtat
     4801 catgttgaga ggcatgattc gctcgaccag caaggcagcc gcgcagtatc aggccagcaa
     4861 gaacagcaat caaagcggcc tggttgcgct tgcagggctg gccgtgacca ttgggacggt
     4921 cgtaactgaa tcggccgatg aacgcggctg gcgctcattg ccaggccaaa tcgcgatcgc
     4981 gcgcctgcgc ataccttcgg gcgagcatac tcttgcattc ggggctaacc ccgggggaca
     5041 gggtctgcgg ttcagcgtat cggggcgcta cgcggtgatc agtttgcgat tgatcggcaa
     5101 tgccgctttt ctaattccat ccccggtggt accgtccgaa aagtccggta cgcaagcatc
     5161 aaatccactg cctcttgtgg tgcagtcaac gacgacggca tcgaaaacgt ttggacaacc
     5221 gtgagtcact ctaggagcga aacatgaaac tgaccgtgct tgccgctatt ctcactgcat
     5281 cgataatcgt cgccggatgc ggaggcaaca agttcgagcg ccccggatat gcctacggct
     5341 gcccgaccgg cgacgacaag aagatcaagt ccaaggaaga cttgatcaaa gcgaaactcg
     5401 aagtagcggg ttcgcttccg gatatcgatg tggtggattt gcgctgctcg gaccgttccg
     5461 ccctgttgcg cgtcgatatg gatctgaaga acgacagcaa ggaagtacgc cgcatcgcct
     5521 atcggttccg ctggcttgat aaggagggta tgcgcgcatg ggacgatgag tcatggaaac
     5581 cgctgctgat ttacggcaaa acgctttaca ccgtgacgac aatgtcgcct tcgcaagaag
     5641 ccactgactt cagggtcgta gtcatggacc aggacaaata acaatgctta cctcaaggag
     5701 cgatatgatg actcaatctc ttcccgttcg gctcgcgagc atattcgcat tctgcgtggc
     5761 agttggcctg tccggctgcg cgtcggagaa aacgaaatac ggcgatgcgc gcggcgtcga
     5821 aaccgtgacc aaccagttcg gctccacgga tttgcagatg atcgcggaat ccatgacgcg
     5881 ctccatggtc aatgcgccgg tagtggcagc ggggaatctg ccgatcatga ccgtgcagga
     5941 ggtcaagaac aagaccagcg agtacattga cacgcgtgcg atcacggatt ccatccgcgt
     6001 cgaattgcag aaaagcggcc gtgtccggtt tgcggcggac gcggccgcga tggatcagca
     6061 ggtcgaggaa ttgaagaggc aacaaagcga ctattacgat ccaaagaaat cgtccgaaat
     6121 aggcaaaatg gtcggtgcgg cctaccgcct tgagggcaat atttcttcta tcgtgaagga
     6181 agccaagggc gtcaaggaca tctactacaa gttcaatctg caactctgga atatccaaaa
     6241 cggcctgctg gaatggactg acgaaaaaga aatccgcaag accacgtcca aaacctgacg
     6301 tccgtagtca aattgagagc gcccggtgga accgccgggc gcttttattt tcgcgggctg
     6361 catcaaggcg acactcggaa ggcgatcgaa cagtccggcc ggtcaatctc attaccatgc
     6421 acacgggaaa ccatgcgcag cgcttttcgt ctgcgagccg catttttccg ataagctgaa
     6481 gaaaaagccg catccaaggc cagcggctat tcgaacggac caaagtccag ctcctggaga
     6541 gctggcctcg gaggaacaat gcgacgaggt ttcatcagga agaagcccca gtgatagagg
     6601 tccggggcct caccatgcgt tatggagaac ttgccgcgct aaccgatgtt agcctcgacg
     6661 tagcggccgg tgaaatactg gtggtgctcg gtccatcggg ggctgggaag tccacgctgc
     6721 ttcgctgcat caaccggctg atcgaaccga gtgccggaga agttcgcatg gcgggcgtac
     6781 caactcccgg cgatcgcact ggattgcgtc gactgcgttc ccaaatcgga atgatttttc
     6841 aggatcacaa cctggttcct aatctgtcgg tactcaaaaa tgttttgacg ggccgcctgt
     6901 cccacatgcc cacctggctt tcggtgctgc aactattcac cgacgcagac gtggacatcg
     6961 ccttggagtg cctaagacgc gtggatctcg aggcacgtgc ctggaaccgc gccaatcggt
     7021 tgtccggcgg ccagcaacag cgcgtcggca ttgcccgcgc cctggcccag cggccgcgcg
     7081 ctatcctcgc cgatgagccg gtagcaagcc ttgacccgca gagcgcgcgc attgttctgg
     7141 atgacctgca ccgtgcggca cgcgatcttt ccatagcggt gctctgcaac cttcatcagg
     7201 ttgagtacgc ccgcgatttt gcggatcgca tagtcggcat ttcggcaggc cgggtggtat
     7261 tcgagggtac gcctgcacaa ctcgataatg ctgcgctggc ccggatctat ccaggcctga
     7321 attccactag tggaggagaa tcatcttcgc ggcggagcgc ggatgtcgag cgatctacgg
     7381 cggtgcagta agaatgaacc cgcagcggct tcgatttatt ctgcatcgtc catggacggc
     7441 tcgccattgg accaagtgtg gtcttgttgc cggcgtaact gcggctgcat tattttggag
     7501 tgcgcattat cttggtctgt cgttgcctga actcgtaaat ggagcgccat ggattgcaga
     7561 tctcgtttcc agaatgtttc cacccaactg gggatttttg cggcagcttg gcaagccggt
     7621 tctcgaaacc atccagatag ccgtatgggg aacctttttt tcggccgcgc tcgctttgcc
     7681 actctgtttc ctggcggccc gcaacatggt cccgcgatgg atcttccaca ccacgcggca
     7741 gttttttaac gcggcccgcg gtattaatga gctcatattc gcgctgatct ttgtggcggc
     7801 ggttggtttg ggcccgttcg ccggggtgct tgcgctttcg ctgcatggtg ccggcatgct
     7861 cggcaagttt ttcgcagagg ccgtggagga agccgatcgc gggccgattg aggcgttgcg
     7921 cgccaccggt gcttcagcca cccaagtggt gacattcgga attttgccgc aggtcctgcc
     7981 agcctggatc gccgcgacgc tgtaccggct ggaggtcaat ctgcgcgccg caaccgtact
     8041 cggcatggtt ggggcggggg gcatcggttt tgagctttac agcagtttga aattgttcca
     8101 gtaccaggat acagctacct gtgtactggt aatcctcgcc atggttatga gtgcggatta
     8161 cctgtcgtca cgtctgcggg ctcgtattct cgcgacctga ttccgagcag gcggcagcgt
     8221 gttcggacgg tgccgacctc cgatatccaa cctgccgcct gctttagatc cttccaagac
     8281 attgacatga atcaatgccg acacgatcgg ttcggatcag gctgccagat gaatacgcta
     8341 tcagacgagc ggtggaatca gagtcacgct tgcgcactgg cgggaaatca tcacggcaga
     8401 tgctcagatc gtcgcggtgc agtgaggaga aggaaaatta cat
//
LOCUS       DATMZQ010000223         2522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2159026, whole genome shotgun sequence.
ACCESSION   DATMZQ010000223 DATMZQ010000000
VERSION     DATMZQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159026"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VN496_07285"
     CDS             complement(<1..698)
                     /locus_tag="VN496_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXF16790.1"
                     /translation="MLYQFLSDNKPNLIERCRVKAAKRPMPEQTKEALLHGMPVFIQQ
                     LIDTLQMEQEPKAFSERRASISPAGGRRRPTEMSKSATIHGHELLDHGFTVDQVVHAY
                     GDVCQSVTDLAFELGEPFQIDEFRTLNRCLDDSIADAVMEFTYQRDTLNADKQSLKMS
                     LRIGTLAHELRNLIQTATLSFAAIKAGTAGLSGSTGAMMDRTLVAMRDMVDGSVAEVR
                     MDAGMPMHSQMFALA"
     gene            1052..1804
                     /locus_tag="VN496_07290"
     CDS             1052..1804
                     /locus_tag="VN496_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXF16791.1"
                     /translation="MSAPNPHPPTKNHLIAALPAASYERLLPHFDPVVLPLGWSVYES
                     GDRLAHVYFPTEGIVSLLYVMEDGASAEIAITGNEGPVGISLFMGGESTISRAVVQSA
                     GYAYRLNAALVKSEFARGDEFQQLLLRFTQALITQMSQTAVCNRHHTVEQQLCRWLLL
                     SLDRLPTNQLAMTQELIANMLGVRRGGVTEAAGHLQAAGLIEYARGKITVLDRPKLED
                     RACECYAVVKRESDRLLSHFSASSSPRKAAEA"
     gene            1990..>2522
                     /locus_tag="VN496_07295"
     CDS             1990..>2522
                     /locus_tag="VN496_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16792.1"
                     /translation="MKKIKNVASQPLRKTGTGIPEFDGLSRGGLPADPTRLLKGGPRW
                     RKKAFALQTANRLLAALPRRELKILLAYLSPVTLTYGEVLSKPGEKIRYVYFPNDCIV
                     SLLTTVARHQSLEVGMVGPEGMIGIPLALGVSVSPVRALVQGSGTALRMTAANFISAL
                     RNSPPLQKELYRFTFAK"
BASE COUNT          543 a          705 c          704 g          570 t
ORIGIN      
        1 gccagagcga acatctggct atgcatgggc atgccagcgt ccatgcgaac ctcggcaact
       61 gagccatcta ccatgtcgcg cattgcaacc aatgtgcggt ccatcattgc gcccgtagac
      121 cctgacaatc cggccgtgcc ggctttgatt gccgcaaagc tcagcgtggc ggtctggatg
      181 agattacgca gttcatgagc gagtgtgcct atccggagac tcattttcag gctctgcttg
      241 tccgcgttaa gcgtatcccg ctgatacgtg aattccatga cggcatcagc aatggaatca
      301 tccagacagc gattcaaagt tcgaaactcg tctatctgga atggctcacc cagttcgaac
      361 gccagatcgg ttacggactg gcagacatct ccgtaggcat gcactacctg gtcaacagtg
      421 aagccgtgat ccagcagttc atggccatgg atcgtggccg acttgctcat ttcagtaggg
      481 cgacgccttc ctcccgcagg ggagatggaa gcacggcgct cactgaaggc ttttggctct
      541 tgctccattt gcagcgtgtc aataagttgc tgaatgaaga ccggcattcc gtgaagcagc
      601 gcttctttgg tttgctccgg catgggtcgt tttgccgcct tcacgcggca gcgctcgatc
      661 agattgggtt tgttatctga caggaattgg taaagcacgg acacctcctc aatgacctct
      721 cacgttagtc ggcgtgccct accgcgtatg tgcgctaccg ggcttataga atctttttta
      781 cacaccatcg ctcttcgcca aagatcgcgg tgcgcgatat gcagcagagt gccgtaaggc
      841 cgtttggagc ccgaacttga tggtcgtccc gcggtcggat gctatgcgat atctcgaccg
      901 actgcccttc ggcctgggcg gggaagctgt gacccgaatt gcagacgtat ccggaagcag
      961 cacggccaag cctgaagact ggaggcagat gtagcggacg ccgacattta aggtgtacgc
     1021 taccgtacat ataccggttg aacgagcccg tatgtccgcg cccaatccgc accctcctac
     1081 gaaaaaccat cttatcgccg cactccctgc ggcgagctac gagcgcctgc tgccccactt
     1141 cgatccggtt gtgcttccgc ttggctggtc tgtatatgaa tccggcgacc gcctggcgca
     1201 tgtgtatttt cctaccgagg ggattgtctc cctgctatac gtgatggaag acggcgcctc
     1261 cgcagaaatt gcgatcacgg gaaatgaagg cccggtaggc atttccttgt ttatgggagg
     1321 agagagcacc ataagccggg ctgtggtgca aagcgcggga tacgcatatc gcctgaacgc
     1381 ggcgctggtg aaatcggaat tcgcacgcgg cgacgaattt caacaactgc tcttacgctt
     1441 cacgcaggca ttgatcaccc aaatgtcgca gacagccgtg tgtaatcgac atcacacggt
     1501 cgagcaacag ctatgccgtt ggttgctgtt gagcctggat cgattaccca ccaaccaatt
     1561 ggccatgacc caggagttga ttgcgaacat gctgggtgtg cgccgcggcg gggtaacgga
     1621 agctgcggga caccttcagg ctgctggctt gattgagtac gcccggggca aaatcaccgt
     1681 actggaccgg cctaagctgg aggaccgagc gtgcgagtgc tatgcagtgg ttaaacgcga
     1741 atcggaccgt ttgctatcgc atttcagcgc ttcgtcaagt cctcgcaaag cggccgaagc
     1801 ttagctcacg taacagttca tctggctgtc ggtccgttag cgtacatacg gaagactatc
     1861 tttatcttcg tccgacagca ccgcagtaga cgcggcaggt ctatagtctc ctcaaccata
     1921 tctggaaatc ggtccacacg taaaaggggg cttatagtct ggccgttttt aacttttgaa
     1981 gggatgccag tgaaaaaaat aaaaaacgtg gcgtcccagc ctttgcgcaa aacagggacg
     2041 ggtataccgg aattcgatgg attgagccgt ggcggcctgc ccgccgatcc cacgaggcta
     2101 ctcaagggcg ggccacgttg gcgcaagaaa gcatttgccc tgcaaaccgc gaacaggttg
     2161 ctggcggcct tgccgcgcag agaactcaag attctgctcg cttacctttc tccggtcacg
     2221 ctgacttatg gcgaagttct ctccaagcct ggagagaaga ttcgatacgt ctattttccg
     2281 aatgattgca tcgtgtcctt attgacaacc gtggcacgac atcagtctct ggaagtgggc
     2341 atggtcggtc cagaaggcat gatcggaatt ccgcttgcat taggcgttag cgtctctccc
     2401 gtacgcgcgc tcgtgcaggg atccggcacc gccttgcgga tgaccgcggc caatttcatc
     2461 agtgcattgc gcaacagccc gccattgcag aaagagctgt accgctttac ctttgccaag
     2521 tt
//
LOCUS       DATMZQ010000224         4939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3279257, whole genome shotgun sequence.
ACCESSION   DATMZQ010000224 DATMZQ010000000
VERSION     DATMZQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3279257"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..946
                     /locus_tag="VN496_07300"
     CDS             149..946
                     /locus_tag="VN496_07300"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXF16793.1"
                     /translation="MDQFVIAGKQYTSRLLVGTGKYRDFEQTRAAIDASGAEIVTVAI
                     RRTNIGQNKGEANLLDVLEPGRHTILPNTAGCYTANDAVRTLRLARELLDGHDLVKLE
                     VLGDPDTLYPNMIETLAAAETLVHEGFKVMVYSSDDPILAHQLEQIGCIAIMPLASLI
                     GSGMGILNPWNLQIIIKNATVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAKDP
                     VLMASAMKKAVQAGREAWLAGRMPKKLYEAAPSSPPEGVIASRGKAA"
     gene            989..1705
                     /gene="trmB"
                     /locus_tag="VN496_07305"
     CDS             989..1705
                     /gene="trmB"
                     /locus_tag="VN496_07305"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXF16794.1"
                     /translation="MGDGNCGTVYWRPVNAPQRHIRSFVLRQGRVSNAQRRALDVLLP
                     RFGIPYADSPYDFDAAFGRSAPRFLEIGFGMGETTARIAAAHPQNDYIGVEVHTPGVG
                     ALLREIEAQQLSNIRIIQHDAVEVVARMISATSLSGIHLFFPDPWPKKRHHKRRLLQS
                     PFVHLLASRLQSGGYLHVATDWEEYAAEILNVCSAEPGLVNTAAEFAPRPAYRPQTKF
                     ESRGVKLGHGVWDIVFRLRH"
     gene            complement(1736..2959)
                     /gene="hemW"
                     /locus_tag="VN496_07310"
     CDS             complement(1736..2959)
                     /gene="hemW"
                     /locus_tag="VN496_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXF16795.1"
                     /translation="MQILQSPATDRTVALQSLPPLSLYVHIPWCVRKCPYCDFNSHEV
                     KASASSDSPTRLNLDSALEQRYVAALIADLESTVPQVWGRPVYTVFLGGGTPSLLSPR
                     ALDDLLSAIRARLPLAPDAEITLEANPGTFEAGKFAEFRAIGINRLSIGIQSFNSEHL
                     RALGRIHDATQARTAIEIAQSSFDNYNLDLMYALPGQTVKQAVADIETALSFSPAHIS
                     AYHLTIEPNTLFYRHPPMLPDDDVSADMQQALETRLAHVGYEHYETSAFAKTGRQARH
                     NLNYWTFGDYLGIGAGAHGKLSFADRIVRQMRYKQPRQYMDQAETGAAVQQEQEVRAS
                     DLPFEFMMNALRLTGGFELSSFVARTGLPVYVLRKELQEAESRGLIERDYVTVQPTLK
                     GQRFLNDLLQIFLKT"
     gene            3043..3846
                     /gene="murI"
                     /locus_tag="VN496_07315"
     CDS             3043..3846
                     /gene="murI"
                     /locus_tag="VN496_07315"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXF16796.1"
                     /translation="MMPSEDESPIGFFDSGIGGLTILRAVRRALPCEHLIYVADAAYV
                     PYGQKSPEQIRARALAIGAFLVQQQAKAIVVACNTATAAAIDVLRTSVPIPVIGIEPA
                     VKPAVTATRSGVVGVLATPATLASERFRNLVERFACGIRVLVQPCAGLAEHIERGDLD
                     GQNTEQLVRGFVQPLLKQGADVIVLGCTHYPLVAHIVQDIAGPAVAVIENGTAVAKEV
                     AKQLALQGSARAGGTGKEQFWSSGPDQGIAPQLKTLWSPIVTLRRLPEF"
     gene            complement(3858..4151)
                     /locus_tag="VN496_07320"
     CDS             complement(3858..4151)
                     /locus_tag="VN496_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16797.1"
                     /translation="MNARLRSIVHRRRLLIVLATEQRGEFALQAGALRRSLALADLAW
                     RGYRRLKSSPVVMAVAAVALLTVGPGRLLSVAYRSGLLVQGLLRLAKLFRTLR"
     gene            complement(4148..4552)
                     /locus_tag="VN496_07325"
     CDS             complement(4148..4552)
                     /locus_tag="VN496_07325"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXF16798.1"
                     /translation="MENPQNLKAAQGQLPGIFDSLKQLLGTLVAIAQTRIELLGTEVE
                     EQVARLVSMLLWTIVSLFCGFTAAILIAIVVLITFWDSNRILAAVLLVAGYAALGLFS
                     WLRVRALARARPRLFEATLGELAKDRDRLGGT"
     gene            complement(4563..4871)
                     /locus_tag="VN496_07330"
     CDS             complement(4563..4871)
                     /locus_tag="VN496_07330"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HXF16799.1"
                     /translation="MNSKTVSADKLMEDLRLVVTDAEDLLKATAGQAGEKVAAARART
                     AESIDAAKARIAQAGYAAAAQTREAAKVADDYVRDNPWTAVGVAAALGVLIGMLIGKK
                     "
BASE COUNT         1019 a         1464 c         1445 g         1011 t
ORIGIN      
        1 gcgcggcacg tttgcaacca tggaactcca ggatggggat gcactcgaaa ttgtggtcgc
       61 ggtgggggga ggataagcac gatggccgtt gcattgaccc gcacaattgg caaaccgcta
      121 tcacttaaac taaagcacta ggtcatccat ggatcaattt gtcatcgcag gcaagcagta
      181 cacatcgcgt ttgctggtgg gaaccggcaa gtacagggat tttgaacaga cgcgtgcggc
      241 aatcgacgcg agcggcgcag aaatcgtgac cgtggcaatc cgtcgaacca acatcggtca
      301 aaacaaaggc gaggctaacc tgctcgatgt gctggagcct ggccgtcata ccatcttgcc
      361 caatactgcc ggctgttata cggcgaatga cgcagtgcgt accctgcgtc tggcgcgcga
      421 gctgctcgat ggccatgacc tggtgaaact ggaagtgctt ggcgacccgg acacgctcta
      481 cccgaatatg attgagacgc tcgctgcggc agaaacgctg gtgcacgaag gtttcaaggt
      541 catggtgtac agctcggacg atccgatcct ggcacatcaa ttggagcaga ttggttgtat
      601 cgccatcatg ccgctggctt cgttgattgg ctctggcatg ggcatcttga acccatggaa
      661 tctgcagatc attatcaaaa acgcgactgt tccggtgctc gtggatgccg gcgtcggcac
      721 ggcttcagat gccgccattg ccatggaact cggttgcgat ggcgtgttga tgaacaccgc
      781 cattgcggcg gccaaagatc cggtcctgat ggcatccgcg atgaaaaagg cagttcaggc
      841 ggggcgcgaa gcctggctcg cgggacgcat gccgaagaaa ctgtatgaag ctgctccgtc
      901 ttcaccgcct gaaggggtta tcgccagccg cggcaaggca gcttagcgcc cgcgtggcgt
      961 cggcccgcat gtagtgccag ctcgatgcat tggcgatggc aactgcggaa cggtatattg
     1021 gcgcccggtg aacgccccgc aacgacatat ccgcagcttc gtgttgcgcc aaggccgtgt
     1081 ctcgaatgcg caacgtcgcg cgctcgatgt cctgttgcct cgcttcggca ttccgtatgc
     1141 ggattcaccc tatgactttg atgccgcgtt tggacggtct gcccccagat ttcttgaaat
     1201 cggttttggc atgggcgaaa ccaccgcgcg gattgccgcc gcacatccgc aaaacgatta
     1261 catcggtgtc gaagtgcata cccctggtgt cggtgcactg ctacgggaga tcgaggcgca
     1321 acaactgagc aacatccgca tcatccagca cgatgccgtg gaagtcgttg cgcgcatgat
     1381 ttccgcaacc agcttgtcgg gcatccactt gttttttccg gatccgtggc caaaaaaacg
     1441 ccaccataag cgccggctcc tgcagtcgcc gtttgtgcat ttgctggcca gtcggctgca
     1501 gtccggcgga taccttcacg ttgcaaccga ctgggaagag tatgccgcag agatattgaa
     1561 cgtgtgctcc gccgagccgg ggctggtcaa tactgccgcg gaatttgcac cgcggccggc
     1621 gtatcgtccg cagaccaaat tcgagtcgcg cggggtaaaa ctcggccacg gtgtttggga
     1681 catcgtgttc cgccttcgac actagcttgt gccgtcgccc cggcgcagcg ataccctaag
     1741 tttttaaaaa aatctgcagc agatcattca ggaaccgctg gcccttgagc gtcggctgca
     1801 ccgttacgta gtctcgctcg atcaatcccc gggattccgc ttcctggagt tctttgcgta
     1861 gcacgtacac aggcagaccg gttcgcgcca cgaacgagga cagctcgaac ccacccgtga
     1921 gccgcagtgc gttcatcatg aactcgaatg gcaggtcgct tgcacgaact tcctgttcct
     1981 gctgaactgc ggcacctgtt tccgcctggt ccatgtactg acgcggctgc ttgtagcgca
     2041 tctgtcgcac gatgcgatca gcgaacgata atttgccatg cgcgccggca ccaatgccga
     2101 gataatcacc aaaagtccag tagttcaaat tgtgtcgcgc ctgacgaccg gttttggcaa
     2161 aggccgatgt ttcgtagtgt tcatagccaa cgtgtgcaag tcgcgtttcg agggcctgct
     2221 gcatgtccgc cgacacatca tcgtctggaa gcatcggtgg atgccggtaa aacagcgtgt
     2281 tcggctcaat ggtcagatgg taggccgaga tatgggccgg cgaaaaactg agcgcagtct
     2341 cgatgtcggc aacagcctgt ttcacggtct ggccggggag tgcatacatc aagtcgaggt
     2401 tgtaattgtc gaagctcgat tgcgcaatct cgatggcggt gcgcgcctgg gtcgcatcgt
     2461 gaatgcggcc aagtgcacga aggtgttcgc tattgaaact ctggatgccg atcgacagcc
     2521 ggttaatgcc gatcgcgcgg aattcggcga acttgcctgc ctcaaatgtt ccgggatttg
     2581 cctccagcgt aatttcggca tcgggtgcca gtggcaagcg tgcgcggatg gcagatagaa
     2641 ggtcgtcgag cgcgcgcggc gacagtaatg acggcgtgcc accgccgaga aacacggtgt
     2701 acaccggccg cccccaaact tgcggcacgg tggactccag gtcggcaatc aacgcggcaa
     2761 catagcgttg ctccagcgca ctgtcgagat tcaaccgcgt ggggctgtcc gagctggccg
     2821 atgctttcac ttcgtgcgaa ttgaaatcgc agtaggggca tttccgaacg caccatggga
     2881 tatggacata caaagacagc ggcggcaatg actgcagcgc gactgttcta tccgtggcgg
     2941 gggactgcag gatctgcatt ggctgttgaa ggatccgagt tttctactat aacctgtccg
     3001 tcacttgctc catcggctcc gcatacctgc cttaccagcg caatgatgcc atctgaagat
     3061 gaatcgccga tcggcttctt cgattcgggc atcggcggcc tcacgatcct gcgcgccgtg
     3121 cggcgcgccc tgccgtgcga acacttgatc tacgtggctg atgcagccta tgtgccctat
     3181 ggacagaaat cgccggagca gattcgcgct cgcgccctgg cgatcggtgc atttttggtc
     3241 caacaacagg ccaaagccat cgtcgtggcc tgcaatacgg ctaccgctgc cgcaatcgat
     3301 gtactgcgta ctagcgtgcc gatcccggtt atcggcatcg aaccggcggt caagccggcg
     3361 gtgactgcga cccgcagtgg cgtagtcggc gtgctggcca cacctgcgac gctggcgagc
     3421 gagcgctttc gcaatctggt agagcgtttt gcttgcggca tccgcgttct ggtgcagccc
     3481 tgcgccggcc tggccgaaca tatcgagcga ggcgaccttg atgggcaaaa caccgagcaa
     3541 ttagtgcgcg gttttgtcca gccgctactg aaacaaggcg cggacgtgat cgtgctcggt
     3601 tgcacacact acccgctggt cgcgcatatc gtccaggaca ttgccgggcc ggcagtagcc
     3661 gtaattgaaa atggcactgc cgtggccaag gaagtggcga aacaacttgc gctgcagggc
     3721 tccgcgcgcg caggcggcac gggaaaggag cagttttggt cgagtggacc cgaccaagga
     3781 atcgcgcctc agctgaaaac tctttggtcg ccgatcgtca ccctacggcg cctgccggag
     3841 ttttagaggg gcacgcttta acgcaatgtc ctgaacagct tggcgagccg cagcaatccc
     3901 tggaccagta atccactgcg ataggcaacg ctcagtaatc ttccgggccc taccgtcaac
     3961 agcgccaccg cggccacggc cattaccacc ggactcgatt tgagtcggcg gtaaccacgc
     4021 cacgcaagat cagccagcgc aagcgaacgg cgcaacgcac cggcttgcag cgcaaattcg
     4081 ccacgctgct cggttgccag gactataagc agccggcgtc tgtgtacgat actgcgcaat
     4141 cgggcgttca tgttccgccc aatcgatcgc gatccttggc aagttccccg agcgtcgcct
     4201 cgaacagccg cggtcttgcc cgagcaagcg ccctgacgcg caaccaggaa aacaggccga
     4261 gggcggcgta tcccgccacc agcagcaccg cagcgagaat gcgattggaa tcccagaacg
     4321 tgatcaggac aacgattgcg atcaagatgg ccgcggtaaa gccacagaac agcgacacaa
     4381 tagtccatag cagcatcgaa accagacgcg caacctgttc ctcgacttcc gtgccaagca
     4441 gctcgatgcg cgtttgcgca atcgcaacca gggtgccgag cagttgcttc agcgaatcga
     4501 aaatgccggg caattgacct tgcgccgctt tcagattctg tggattttcc atgtgctgca
     4561 gctcacttct taccgatcag catgccgatc aacacgccca atgccgccgc aacgcccaca
     4621 gcagtccagg ggttgtcgcg cacatagtcg tccgccacct tggcagcctc gcgggtctgc
     4681 gcagcggcgg cataaccggc ctgcgcaatg cgagccttgg cagcatctat ggattcagct
     4741 gtccgcgccc tggcggcagc tactttttcg cccgcctgcc cggctgttgc tttgagcaaa
     4801 tcttcggcat ccgtgactac gagccgcagg tcttccatta atttgtccgc cgacaccgtc
     4861 ttgctattca tcacgatctc ctcgaaataa tttggatcac ttcagccaat atggctgtcc
     4921 tcgttcgctg gtgtgtcag
//
LOCUS       DATMZQ010000225         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2588847, whole genome shotgun sequence.
ACCESSION   DATMZQ010000225 DATMZQ010000000
VERSION     DATMZQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588847"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1088
                     /locus_tag="VN496_07335"
     CDS             174..1088
                     /locus_tag="VN496_07335"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF16800.1"
                     /translation="MLISRTITRRAFAMSGALLLFVLVLVARPTSAADEQVWPPITDP
                     PTAKYVPGKWVWEELFTEHAALAADFYGKLFGWTFQVLPAGRGPAYTLALSDGEPVGG
                     MIERDHRYEQQQGSRWIGMISVPDVKAAASYAAKQGGKVIVAPRVLAGRGEVALLTDP
                     EGTPFGVMRSASGDPPDFLAEDGQWVWFELWAKDPGAMTTFYAGLAGYEIESMPRPDG
                     RPGYALSSGGFLRCGIVASPSSSVASAWLPYVRVSDVKAATARAAQSGGRIVLDPSPE
                     VREGRVGLIMDPTGAPVGLVQLPVEDTR"
     gene            1106..1381
                     /locus_tag="VN496_07340"
     CDS             1106..1381
                     /locus_tag="VN496_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16801.1"
                     /translation="MNLRIACIALLIAAGTAGCAGHGSGSASVGFSYGAYYGSPWGAS
                     YHVNGPVYVGPPGNARPPGGSGRPPHAANLPARPMPQPRPSGGGRRR"
     gene            1402..>2030
                     /locus_tag="VN496_07345"
     CDS             1402..>2030
                     /locus_tag="VN496_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16802.1"
                     /translation="MATSSLLDRVASALFGGKKTDTDADRQLIADMIELIVEEVEPRV
                     RFHARYREKLEKCVRASIAYLRSIGSQPLEPVPLSRKSWNDDARVNTFFAKADDVSTC
                     IGRSKELRAFFENPSNAEIQEAYALLGMKKDEHTTFGTQLQGDAVQHDVAQTAVNFSG
                     HRLIAPSATLAATRLEIGRRMIQRLAQIALARIVAIDMKATELQEHKAYL"
BASE COUNT          393 a          598 c          647 g          392 t
ORIGIN      
        1 aacaggcaat gcaatcaagc ctgtatcctg tgcttcgtaa cgcaatgctg caatgcctaa
       61 taactatctg gcgcgcatgg taccgcacga gcgcatcatg ccggccgatg acggaacgaa
      121 ttttgtgaag cagcacgagg agaggctgaa atgttgacag gccagctttc gctatgttga
      181 tcagccgcac catcacacgt cgtgcattcg cgatgtcggg cgcgctgttg ctctttgtgc
      241 tcgtgctagt cgccaggcca acaagtgcgg ctgatgagca ggtgtggccc ccgattaccg
      301 atcctcccac ggcgaaatat gtccccggca agtgggtatg ggaagaattg ttcacggagc
      361 acgcagccct tgccgcggat ttttacggca agttgttcgg ctggacgttt caggtgctgc
      421 ccgcgggacg cggtcccgcg tacacgctcg cattgtccga tggagaaccg gttggcggca
      481 tgatcgagcg cgaccacagg tacgaacagc agcagggcag ccgctggatc ggcatgatct
      541 cggtcccgga cgtcaaggcc gccgcaagtt atgcggcaaa gcagggcgga aaagtcatcg
      601 ttgcgccgcg tgttctcgcg ggccgcggtg aggttgcgct gctgaccgat ccggagggca
      661 cgccgtttgg cgtaatgcgc tctgcaagtg gagatccgcc agactttctg gccgaagatg
      721 gtcaatgggt gtggtttgaa ctttgggcga aagaccccgg cgcgatgacg acgttctatg
      781 ccgggctggc cggctatgaa attgaatcca tgccgaggcc ggacggacgt cctggctacg
      841 cgctgtcctc cggcggcttc ttgcgttgtg gcattgtggc ttcgccttcg tcgtcagttg
      901 cttcggcgtg gctgccttac gtcagggtaa gtgacgtcaa agctgcgacc gcgcgagctg
      961 cgcaatccgg tggacgcatc gtgctggatc catctcccga ggtgcgcgag ggccgcgtcg
     1021 ggctcatcat ggatccaacc ggtgcacccg ttgggctggt acaactgcct gtggaagaca
     1081 cacggtgagc ggcgacacgg agagaatgaa tctgcgaatc gcgtgtatcg cgctgttaat
     1141 cgcggccggt actgcaggct gcgccggcca cggcagcggc tcggccagcg tgggtttcag
     1201 ctatggcgct tactacggca gtccatgggg agcgagttac catgtcaatg gcccggttta
     1261 tgtcggtccg cccggcaacg ctcgccctcc gggaggcagc ggccgcccgc cgcacgccgc
     1321 gaatctgcca gcccgaccaa tgccgcaacc gcgccctagc ggcggcgggc gccggcgcta
     1381 atattacggg aacctatact catggccaca tcctcattgc tggaccgggt tgcctccgcg
     1441 ctgttcggcg gcaaaaagac agacacggac gccgatcgcc agctcatcgc cgacatgatc
     1501 gagctgatcg tggaggaagt cgaaccgcgc gtgcgctttc atgcgcgcta tcgcgaaaaa
     1561 cttgaaaagt gcgtgcgcgc gagcattgcg tatctgcgct caattggaag ccagccgctg
     1621 gagcccgtgc cgctctcacg caaatcatgg aacgatgatg cgcgcgtgaa cacgttcttt
     1681 gccaaggcag atgacgtctc cacgtgcatc gggcgcagca aggaattgcg tgcgtttttc
     1741 gagaacccgt ccaatgccga gattcaggag gcttatgcgt tgctcggcat gaaaaaggat
     1801 gagcacacga cattcggcac gcaactgcag ggggatgcgg tccaacatga cgtggcgcaa
     1861 accgcggtga atttttcggg tcaccggttg atcgcgccat cagccacact ggccgccacg
     1921 cgcctggaaa ttggccgccg catgattcag cgcctcgcgc agatcgcgct tgcccgcatc
     1981 gtcgcgatcg atatgaaggc caccgaactg caggagcaca aggcgtacct
//
LOCUS       DATMZQ010000226         7569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4922496, whole genome shotgun sequence.
ACCESSION   DATMZQ010000226 DATMZQ010000000
VERSION     DATMZQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4922496"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VN496_07350"
     CDS             complement(<1..977)
                     /locus_tag="VN496_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF16803.1"
                     /translation="MMRQVVFWVLVAGVFAVLPFLFSHSFALSMLSQMGIAIIFALSY
                     NMLLGQTGLLSFGHAVYFGLGAFFCAHALNFIGAGKFWFPVTLVPLIGGLAGLVFGLL
                     FGFVTTRRAGTPFAMISLGIAEMVAASALMFTTFFGGEGGIATNRAVGKAFLGLSYGP
                     QIQVYYLIAGWCVVSMIAMFALTRTPLGRMANAVRDNPERAKFVGYNTTRVRWLMMAL
                     ASFFAGIGGGLAAINYEIVTAETLGLVASGNVVLMAFIGGIGQFWGPIIGAVLVTLLQ
                     SALSNYTQAWLLYFGLFFLVIIMFSPGGIANLIVLHQPVWKAGLLKRLFP"
     gene            complement(974..1921)
                     /locus_tag="VN496_07355"
     CDS             complement(974..1921)
                     /locus_tag="VN496_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF16804.1"
                     /translation="MEFFLVTLLNSLSYGLLLFMLSSGLTLIFSMMGVLNFAHASFYM
                     LGAYFAYQTGLYAGFWPALFIAPVAVGALGAATERFGLRTVHKFGHVAELLFTFGLAY
                     VIEEVVHLIWGRAPVDYRIPGQLDFTLFTLYTTQYPAYRGFMMLVSVGMLLALYFGLT
                     RTRIGLVIQASLTHPDMVESLGHNVPRVFMLVFAGGAALAGLAGVIGGNAFITEPGMA
                     TAVGSIVFVVVVFGGMGSLAGAFLASLIIGALQTFSVALDYSMLDVLAKLGIHISATS
                     LWHGFWSITISQTAPVLPYLLMVLMLIVRPRGLMGTRES"
     gene            complement(1925..2125)
                     /locus_tag="VN496_07360"
     CDS             complement(1925..2125)
                     /locus_tag="VN496_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16805.1"
                     /translation="MKSAKSGLPLAIDKAKSNDHPPQLSPALENLGLGDDRGEICIRA
                     GNHQLGGRLYISALAPVAARDA"
     gene            2112..2450
                     /locus_tag="VN496_07365"
     CDS             2112..2450
                     /locus_tag="VN496_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16806.1"
                     /translation="MADFIAIAPRAGQLAGNLAIESAFGTVAGVICLFILFADLFGAA
                     YGHPPTGLSDLPTIILHAYRLLFCMRPNALRARTAGILCAKQRRCKRMTDCRKHGATF
                     FCTATKNRPV"
     gene            2525..4165
                     /locus_tag="VN496_07370"
     CDS             2525..4165
                     /locus_tag="VN496_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HXF16807.1"
                     /translation="MQGLMMDVPLLISSLIKHADRHHGDTEIVSRLVEGGIHRYSYRA
                     SHRRARQLARALGALGVKPGDRVGTLAWNGYRHLESFYGISGMGAVMHTINPRLFPEQ
                     ISYIANHAEDSYVLFDLTFVPLIEKLAPTCKTVKGWVVMTDRGHMPQAGIPNLLCYED
                     LIDAQSDDYEWPQFDERTASSLCYTSGTTGNPKGVLYSHRSTVLHAYAAALPDALNVS
                     ARDVMLPVVPMFHANAWALPYSCALVGAKMVLPGAQLDGKSLYELFETEHVTVSAGVP
                     TVWLALLQHMGQHKLRFSTMNRTVIGGSACPPAMIRSFQDDYGVQVLHAWGMTEMSPI
                     GTVSILKSKHLPLPKDQRYAIQNKQGRVVYGVDMKIVGDDGKELPWDGKAFGNLLVRG
                     PWISNGYFKSEGGDPLRVDNNGVAWFPTGDVATIDADGYMQITDRSKDVIKSGGEWIS
                     SIDLENTAMAHPAVAEAAVIGVVHPKWDERPLLVVVKKKDTELTREQLLQFFEGKIAK
                     WWMPDDVVFVDQLPHTATGKLLKTKLREDFKTYKLPTT"
     gene            complement(4175..4978)
                     /locus_tag="VN496_07375"
     CDS             complement(4175..4978)
                     /locus_tag="VN496_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HXF16808.1"
                     /translation="MLAWRQPPGSLAGLPNLKAVLVLGAGVDLVLSDPELPIDVPIVR
                     LVDGGLPGPMAEYALYSVLHFQRRMSDYFEQQRNTVWRPLEESLARQWPVGVMGLGVI
                     GATVARRILAQEFPVSGWSRTKKTLAGIEVFAGSAGLNAFLSRSRVLVNVLPLTPETF
                     GILNARAFAAMPRGSYVVNIARGGHINQVDLLAALESGQLAGAMLDVFQEEPLPAYHP
                     FWRHPNVIVTPHVAAPTLASEAESQVLENIGRLERGEVPLGIVNRDHGY"
     gene            complement(5217..6050)
                     /gene="panC"
                     /locus_tag="VN496_07380"
     CDS             complement(5217..6050)
                     /gene="panC"
                     /locus_tag="VN496_07380"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXF16809.1"
                     /translation="MEVIKTVAELRERLKRESQVACVPTMGNIHDGHLSLVRIARAQA
                     PVTVTTIFVNRLQFGQGEDFEKYPRTLEEDCAKLAAEGNTIVWAPDEKEIYPEPQTFY
                     IDLPKVANKLEGRFRPGHFRGMATVVLKLFNTVQPQIAVFGKKDYQQLAIVRQMVKQF
                     ALPIKIFPAETVRAEDGLALSSRNRYLSAAERKEAIRLREAILGVRDAVLSGDRNFVA
                     MEYAAGALLARHGWQVDYVVVRNQRAILRPDANDKALVVLGAAKLGQTRLIDNIEVQA
                     P"
     gene            complement(6137..6778)
                     /locus_tag="VN496_07385"
     CDS             complement(6137..6778)
                     /locus_tag="VN496_07385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXF16810.1"
                     /translation="MAPDKYRYIVVEGPIGAGKTSLARQLSAQLGKSLLLEDPNANPF
                     LPRFYEDQRRHALATQLFFLFQRVQQVGGLKQVDLFDGGTVADFILDKDPLFASLTLS
                     DDELHLYQQIYAHLMPQAPTPDLVIYLEASAEKLIERVHRRGVAYERNISEQYLVRLA
                     EAYRTYFYQFSAAPLLIVNSDHLNFVDVADDFDLLLRRIYEMRGPREFFSRGE"
     gene            complement(6768..7253)
                     /gene="folK"
                     /locus_tag="VN496_07390"
     CDS             complement(6768..7253)
                     /gene="folK"
                     /locus_tag="VN496_07390"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXF16811.1"
                     /translation="MAEAFVGLGANLENPLQQVSQAILELGAIERTRLLAVSSLYRSA
                     PVGYADQPDFINAVAKVHSQLSPHQLLDALHTIENRHGRRRSKRNAPRTLDLDLLLYG
                     TLVLHEEGLILPHPRMHERAFVLLPLAEIAPDAELPGRPPLPQLLARVDRSGVVKLNG
                     A"
     gene            complement(7262..>7569)
                     /locus_tag="VN496_07395"
     CDS             complement(7262..>7569)
                     /locus_tag="VN496_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HXF16812.1"
                     /translation="FGADMKDIWALQPRFLGRSGRRPYSVLAHPRFRAAYDFLVLRCD
                     SGEVDAEIAEWWENFQKADEEIRKQMLLPDSGPRRRRRRRKPASAKSPPGAATTGSE"
BASE COUNT         1748 a         2195 c         2135 g         1491 t
ORIGIN      
        1 ggaaacaagc gcttgagcag acctgctttc caaaccggtt gatgcaagac aatcagattc
       61 gcgataccgc cgggggaaaa catgatgatc accagaaaaa acaatccgaa gtacagtagc
      121 caggcttgcg tgtaattgga aagcgccgac tgcagcagcg taaccagcac ggcgccgatg
      181 atcggccccc agaattggcc gatgccaccg ataaacgcca tgagtacgac attgccggac
      241 gccaccaggc cgagggtttc cgcggtaacg atttcatagt taatagcggc caggccgccg
      301 ccaataccgg cgaaaaaact ggcgagcgcc atcatcaacc aacgcacgcg cgtggtgttg
      361 tagccgacga atttggcacg ttccggattg tcgcgaactg cgtttgccat gcgtcccaga
      421 ggggtacgtg tcaatgcgaa catggcaatc atgcttacaa cgcaccatcc cgcgatcagg
      481 taatagacct gaatctgcgg accataactg agtccaagaa acgccttgcc cacagcccgg
      541 ttggtggcaa tgccaccttc accgccgaaa aatgtcgtga acattaatgc gctggctgca
      601 accatttcgg cgatccccag cgagatcatc gcaaacgggg tgccggcgcg gcgcgtggtc
      661 acgaacccga acagcagacc gaacaccagg cccgccaacc caccaatgag gggcacgagg
      721 gtcactggaa accagaattt gcccgcgccg ataaaattca gcgcatgtgc gcagaagaaa
      781 gccccgagtc caaaatagac ggcatggcca aaggacagta gcccggtttg tcctagcagc
      841 atgttgtagg acaacgcaaa gattatggca atacccatct gggacagcat cgacagggcg
      901 aagctatggg aaaaaaggaa tggcagcacg gcgaaaacac ccgctacaag cacccaaaac
      961 acgacctggc gcatcatgac tcccgcgtcc ccatcagtcc gcgcggacgc acaatcagca
     1021 tcaataccat cagcaaataa ggcagtaccg gtgcggtttg cgagatggtg atggaccaga
     1081 atccatgcca caacgatgtc gcggaaatat ggatcccgag ctttgcgagc acatcgagca
     1141 tggagtaatc gagcgctaca gagaaagtct gcagtgcgcc tatgatcaaa gacgccagaa
     1201 aagcaccggc cagcgatccc atgccgccaa agacaacgac gacgaaaaca atgctgccga
     1261 ctgcggtcgc catcccaggc tcagtgatga aagcattgcc gccgatgacg cctgcgagac
     1321 cggccagcgc agcaccacct gcgaatacca gcatgaatac gcgcggaacg ttgtggccga
     1381 gggattcgac catgtcagga tgggtgagtg aagcctggat caccagaccg atacgcgtgc
     1441 gcgtcaggcc aaaatatagc gccaatagca tgccgaccga gacgagcatc ataaaacccc
     1501 ggtaggccgg gtattgcgtg gtgtacaagg tgaacagtgt gaaatcaagc tggcccggaa
     1561 tgcggtaatc aaccggtgcg cgcccccaaa taagatgaac cacttcctcg atgacgtaag
     1621 ccagaccaaa cgtgaacagc agttccgcaa cgtggccaaa cttgtgcacg gtacgtaatc
     1681 cgaaccgctc cgttgccgcg cctagcgctc caaccgccac aggcgcgatg aacaacgccg
     1741 gccagaagcc ggcatagagg ccggtttgat aggcgaaata ggcaccgagc atataaaagc
     1801 ttgcgtgcgc gaagttcagt acgcccatca tgctaaaaat aagcgtcagt ccggaagaca
     1861 gcatgaacaa gagcagtccg taggacaggc tgttcagcag ggtgacgagg aagaattcca
     1921 tgctttaagc gtccctggcg gccactggcg ccagggcgga gatgtacaaa cgccccccca
     1981 attgatgatt gcctgcgcgg atgcagattt caccccggtc atcccctaac ccaagattct
     2041 ccagcgcagg cgaaagctgt gggggatgat cgttgctctt ggctttatcg atagctagcg
     2101 gcaacccgga cttggcggat ttcattgcga tcgctcctcg ggctggacaa ctggccggga
     2161 accttgcgat tgaatcggct tttggcacgg tcgcgggcgt catctgcctg tttattttat
     2221 ttgctgatct gttcggcgct gcttatggcc atccgccgac cggtttgagc gacctaccga
     2281 ctattattct gcatgcctac cgcctactat tctgcatgcg gcctaatgct ctgcgcgccc
     2341 gcacggccgg gattctatgc gcaaagcaac gccgttgtaa acgcatgacc gattgcagaa
     2401 aacacggtgc gaccttcttt tgcactgcaa ctaaaaatcg gccggtgtaa cccggcggcg
     2461 gcgcggcgtg ccatgggttc aagctgctag aattccagcg aatcgaatct tactggagca
     2521 agcaatgcaa ggcctgatga tggatgtccc gctgctgatt tcttcgctta tcaagcacgc
     2581 ggatcgccac cacggcgata ctgaaatcgt gtcgcgcctg gtggaaggcg gaattcatcg
     2641 ctacagctat cgcgcttcac atcgtcgcgc cagacaactg gcccgtgcgc taggggcact
     2701 tggggtgaag ccaggtgacc gcgtcggcac gctggcctgg aacggttaca ggcacctgga
     2761 atctttttac ggcatctcgg gcatgggagc ggtcatgcat accatcaacc cgcgcctctt
     2821 tcccgagcaa atctcctata tcgcaaacca tgccgaagac agttatgtcc tgtttgatct
     2881 gacgtttgtt ccgctgatcg aaaaactcgc gccaacctgc aagaccgtga agggttgggt
     2941 ggtgatgact gaccgggggc acatgccaca agctggcatc ccgaatctgc tgtgctacga
     3001 ggatttgatc gatgcacaat cggacgacta tgagtggccg caattcgatg agcgcaccgc
     3061 ctcgtcgctt tgctatacct ctggcacaac cggcaacccc aagggagtgc tgtactcgca
     3121 tcggtccacg gtgctccatg cctatgccgc tgcgttgcca gacgcgctca acgtttcggc
     3181 acgggatgtg atgctgccgg tagtgccgat gttccacgcc aatgcgtggg ctctgcccta
     3241 cagctgcgca ctggtcggcg caaaaatggt attgcctggc gcacaactcg acggcaagag
     3301 tttgtatgag ctgttcgaga cggaacacgt gaccgtatcg gccggggtac cgaccgtatg
     3361 gctcgcgctt ttgcaacaca tggggcagca caagctgcgc tttagcacca tgaaccgcac
     3421 cgtgatcggc ggttcggcat gtccgcccgc catgatcaga agtttccagg acgactacgg
     3481 tgttcaagtg ctgcatgcct ggggcatgac cgaaatgagc ccgattggca ccgtcagcat
     3541 cttgaagtcc aagcatctgc cgctgccgaa ggaccagaga tacgccatcc agaacaagca
     3601 agggcgcgtc gtgtacggtg tagacatgaa aatagtcggt gacgacggca aggagttgcc
     3661 ctgggatggc aaggcgtttg gaaacctgct ggtgcgcggc ccgtggatca gcaacggata
     3721 tttcaagagc gaaggcggtg acccgttacg tgttgacaat aacggtgtcg cctggtttcc
     3781 caccggggat gtcgcaacca ttgacgccga cggttacatg cagatcaccg accgctccaa
     3841 ggacgtcatc aagtccggcg gagaatggat cagttctatt gacctggaaa atacggccat
     3901 ggcccatcct gcggttgcgg aagcagcagt gatcggcgtt gtgcatccca agtgggatga
     3961 acggccgctg ctcgttgtgg tgaagaagaa agatacagag ttgacccgcg aacaactgct
     4021 gcagtttttt gaaggcaaga tcgccaagtg gtggatgccc gacgatgttg tattcgtcga
     4081 ccaactgccc catacggcga caggcaaatt gctcaagacg aaactgcgcg aggattttaa
     4141 gacttacaag ctgcctacca cgtaactcct ggcgtcagta tccgtggtcg cgattcacaa
     4201 ttccgagtgg cacttcccct ctctcgaggc gaccgatgtt ttccagcact tgcgactcgg
     4261 cttccgaggc aagtgttggc gcggccacat gtggcgtgac aatgacgttc ggatggcgcc
     4321 agaacgggtg atacgcggga agcggttctt cctggaacac gtctagcatg gcgcctgcaa
     4381 gttgtcccga ctcaagcgcg gcaagcaggt ccacttggtt aatgtgccct cccctggcaa
     4441 tgttcacaac gtagctgcct ctcggcattg cggcaaaagc acgggcattt agaatgccga
     4501 atgtctccgg cgtcagcggt agcacgttca ccagcacgcg tgagcgcgac aaaaatgcat
     4561 tgaggccggc cgagccggca aaaacttcta tcccggcaag cgtttttttc gtgcgggacc
     4621 agccagagac cggaaattcc tgtgcaagga ttcgacgcgc gacggtagcg cctataacgc
     4681 cgagacccat tacacccacg ggccattgcc gggcgagcga ctcctcgagt gggcgccaca
     4741 cagtattacg ctgctgctcg aagtagtcgg acatgcgccg ctggaagtgc agtacgctgt
     4801 acagcgcata ttccgccatc ggacccggta gtccgccatc caccaggcgc acgatcggta
     4861 cgtcgattgg caactcagga tcgcttaata ccaaatcgac gccggccccg agcaccagaa
     4921 ccgctttcag gtttggcagg ccggccagcg acccgggcgg ctgcctccat gccaacacat
     4981 aacgaatgtc ctgctttgac ccgtaatcgg gccagacgcg aaacggtata tgcggaaaac
     5041 cccgagcaca ggcttgtcgc catggttcgg aaggctcaga agggctgcag aagagaatcg
     5101 tcacaacgta agcgcgcttt aacaatgaaa ccgcggacgt aaggcgccct ggttttcagc
     5161 attactgtcc gagtctcaat gccagaaaag aatggccaac acgacaggat atgcgatcaa
     5221 ggcgcctgca cttcgatatt gtcaatgagc cgcgtctgcc ctagctttgc ggcaccaagt
     5281 acgaccagag ctttgtcgtt ggcgtcaggg cgcaatatgg cgcgttggtt gcgaaccacc
     5341 acgtaatcga cttgccagcc gtgacgtgcg agcaaggcgc cggccgcgta ttccatcgca
     5401 acgaaattac gatctccact cagtacagcg tcgcggacac ccaagatcgc ttcgcgcaat
     5461 cgtatggctt ccttcctttc cgcagccgac aggtagcgat ttcgagatga aagcgccagg
     5521 ccgtcttcgg cacgcaccgt ttcggccgga aagatcttta tcggcagggc gaactgcttc
     5581 accatttgcc gcacgatggc cagttgctgg tagtccttct tcccgaacac agcgatctgc
     5641 ggctgcaccg tgttgaacaa cttgagcacc accgttgcca tgccgcgaaa atgtcctggg
     5701 cgaaaccgcc cctccagctt gttggcgacc tttggcaagt cgatgtagaa cgtttgcggc
     5761 tcgggatata tttccttctc gtctggagcc catacgatgg tattgccttc ggctgctagt
     5821 ttcgcgcaat cctcttccag cgtgcgtgga tatttttcaa agtcctcgcc ttgtccgaat
     5881 tgcagccggt taacgaatat cgtcgtaacg gtaaccggcg cctgcgcgcg cgcgattcgt
     5941 accaggctta gatgtccgtc atggatattc cccatggttg gcacgcatgc gacctgagat
     6001 tcacgcttca accgttcgcg cagttccgcg actgttttaa tgacttccat cgtttaggac
     6061 caagacgaga cggtgttcag ccggaaggtt gccccacccg ctttcttatt tctcgcctgt
     6121 gccccgcctc ctttttttat tctccgcggc tgaaaaattc gcgcggcccg cgcatttcat
     6181 aaatccggcg caacaataaa tcaaagtcat cagcaacatc aacaaaattc agatggtcgc
     6241 tgttgacgat cagcaacgga gcggccgaaa actgatagaa atacgttcgg taagcttcag
     6301 ccagtcgcac tagatactgc tcggagatat tgcgttcgta cgcgaccccc cgccgatgca
     6361 cccgttcaat gagcttctca gccgaagctt ccagataaat caccagatcc ggcgtgggcg
     6421 cttgcggcat caaatgcgcg tagatttgct gatacaggtg cagttcgtca tcggacagcg
     6481 tcaggctggc gaataatggg tctttgtcga ggataaaatc agcgacggtc ccgccatcga
     6541 acaggtcgac ctgtttaagt ccgccgacct gttgcacgcg ctggaacaga aaaaacaact
     6601 gcgttgccag tgcatggcgc cgctggtctt cgtaaaagcg cggcagaaac ggattcgcat
     6661 tcggatcttc gagcagcagc gacttgccaa gctgcgctga tagctggcgc gcaaggctgg
     6721 tcttgccggc ccctatcggt ccctccacta cgatgtagcg atatttgtca ggcgccattt
     6781 agttttacta cgccgctgcg atcgacccgc gcgagtagct gtggcaatgg aggccgtccg
     6841 ggcagttcag cgtccggagc gatttccgcc agcggcaata gcacgaaggc ccgctcgtgc
     6901 atgcgcggat gcggcagaat cagtccctcc tcatggagca ccaacgtgcc gtacagcaac
     6961 aaatcgaggt ccagcgtgcg cggcgcattg cgcttcgagc gccggcgtcc gtgccggttt
     7021 tcgatggtat gcagcgcatc gagcagttga tgcggcgaca attgtgaatg cactttggcc
     7081 acagcattga tgaagtcagg ctggtcggca tagccgacgg gggcactgcg atacaaagaa
     7141 gacactgcga gcaaccgtgt gcgttcgatc gcacccagtt ccagaatggc ttgactgacc
     7201 tgctgcagag gattttccag attggcgccc agcccgacaa aagcctccgc catgcgcgcg
     7261 atcattcgga accggtggta gcggctccgg gcggtgactt ggcgctcgct ggcttgcggc
     7321 gacgcctgcg ccgccgtgga ccgctatcgg gcaacagcat ctgcttgcgg atttcttcat
     7381 cggctttctg gaagttttcc caccattccg cgatttcggc atcgacttca ccgctatcac
     7441 agcgcaggac cagaaaatcg tacgcggccc gaaagcgcgg atgtgcaagc acgctgtacg
     7501 ggcggcgtcc ggagcggcca aggaagcggg gctgcagcgc ccagatatct ttcatgtcgg
     7561 caccgaagc
//
LOCUS       DATMZQ010000227         5671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_780007, whole genome shotgun sequence.
ACCESSION   DATMZQ010000227 DATMZQ010000000
VERSION     DATMZQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780007"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..408)
                     /locus_tag="VN496_07400"
     CDS             complement(40..408)
                     /locus_tag="VN496_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HXF16813.1"
                     /translation="MKPEKNFTASANRRAASIADALGAVANIRAGIPSAVVFEHGTLQ
                     VKMYCPAAEDLQTPHARDELYIVARGSGWFRSGRERHMCKVGDMLFVPARTEHRFEDF
                     SGDFCTWVMFYGPDGGESPG"
     gene            complement(417..1004)
                     /locus_tag="VN496_07405"
     CDS             complement(417..1004)
                     /locus_tag="VN496_07405"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXF16814.1"
                     /translation="MRSQATMQRCHWATGGTPNYLEYHDREWGVPQHDDTTLFEFMIL
                     EGAQAGLSWSTILNKRENYRVAFSGFDPRKIAKYDQGRIERLLQNPGIVRNRLKVESA
                     VANARACLAVQAEFQSLDAYLWQFVQGKAQRNAWTSARQVPAFSAQSDAMSKDLKRRG
                     FKFVGSTIMYAFMQAVGMVNDHTVDCFRWKQLTGK"
     gene            complement(1031..1471)
                     /locus_tag="VN496_07410"
     CDS             complement(1031..1471)
                     /locus_tag="VN496_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16815.1"
                     /translation="MSGVSTHVFARINFLLGFGVLVLLTFYFAAAYLLIPALSAIAPE
                     WAKPAAGVAILAAVGLTVQLLSTALTGLRGIALYKKGEKPSLSAYASQAIMLLSHAAV
                     ACALGSLLYGKPVGDAQLIAIAAAYLVGTGLAIHEWRRRMPARV"
     gene            1693..2241
                     /locus_tag="VN496_07415"
     CDS             1693..2241
                     /locus_tag="VN496_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXF16816.1"
                     /translation="MASSKAPAKSGRQDATQILAADHKKVSKIFAEFEKIKDRDSEGK
                     QQLVKKACDELTVHAQVEEEIFYPALHEALKESDQDLVDEAEVEHASIKQLIETLKTC
                     DPEDRHYDASVMVLSEYVKHHVKEEQDEIFPKARKAKDLDLKQMGEEIMARKMQLMEE
                     HGMEMEDMPASRKRQPASQRHA"
     gene            2336..3469
                     /locus_tag="VN496_07420"
     CDS             2336..3469
                     /locus_tag="VN496_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HXF16817.1"
                     /translation="MQSARKVGLRYVTDAKPGIRRERLRGRFRYIDPSGRTIKDQTTL
                     ARIKALVIPPAWSEVWICSDPNGHMQVTARDARGRKQYRYHALWRKARDENKYEHLLS
                     FANALPAIRRQVDKDLARPGLPRAKILATIVRLLETTMMRVGNEEYARSNGSFGLTTL
                     RDKHVQIHGRRLQFQFRGKSGVQHFVELNDRRLARIVRRCRDLPGYELFQYLDEEGNC
                     RTIDSGDVNDYLREISGCDYTAKDFRTWAGTLLAAMALREFETFDSHAQAKKNMIRAI
                     ESVAEKLGNTPAVCRKCYIHPAIMESYLEGSMLETLKHRAEEKLVHELQDLNAEEAAV
                     LALLQQRLQREQRKPASRRHTGAKTQPARRPARVAERRLQSPI"
     gene            complement(3454..3891)
                     /locus_tag="VN496_07425"
     CDS             complement(3454..3891)
                     /locus_tag="VN496_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF16818.1"
                     /translation="MQLSLPALALCSGSENIMEIDRIDHLVLTVRDIDNTCAFYAGTL
                     GMKIVNFGAGRKALAFGSQKINLHQQGKEFEPKADCPKPGSGDFCLITSVPMQQVTAH
                     LQAHGVALIEGPVKRTGAVGTLLSVYFRDPDLNLIEVSNYIGD"
     gene            3955..5061
                     /locus_tag="VN496_07430"
     CDS             3955..5061
                     /locus_tag="VN496_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF16819.1"
                     /translation="MNSSWTRRDFLIGAGAALAATQAIDHFDPCCAMAADSNTAEDEL
                     FELKPVADGIYAAIAAPRYKVNCNAAVIVTNDGVVVVDSHSKPSAAFALYREIQSVTR
                     QPIKRIINTHFHWDHWQGNQVYAQAFPGLEIITSERTRDNLTRPGAGSGGVPYIEKQL
                     ELVPKEIEKLGNELASATDPAQVMRLRANLRQTEAYLEELRQLKPALPTRTFDQTVSL
                     NVGEREIQILLLGRGHTDGDVFIHLPKEKVVATGDALIDWMPFMNDGYPEDWVRTLDA
                     LEKFEFTKIIPGHGGVMPRDHLTFFRSYLTDLVAAIKKAHGDGATLDEMKRSLPDQLA
                     TKYEAGMSKHPLGQYRDRIGLNIEVAYNKVIKKA"
     gene            5220..>5671
                     /locus_tag="VN496_07435"
     CDS             5220..>5671
                     /locus_tag="VN496_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HXF16820.1"
                     /translation="MRMDLVIVRPEGLYCPPGDFHIDPWRPVERAVITHAHGDHARPG
                     SAKYLATDDSACVLRKRLGDAALDVAPYGDSFNLNGVKVSFHPAGHVLGSAQVRIEYG
                     GEVWVVSGDYKLEGDTTCLAFEPVRCHTFVTESTFGLPIYRWMPQRQI"
BASE COUNT         1303 a         1592 c         1656 g         1120 t
ORIGIN      
        1 gtcctatttg ccggattgtt cagattcgtc tttcagaggt tagcctggcg actctccgcc
       61 gtcaggcccg taaaacatga cccaggtgca gaaatcgccc gagaagtctt caaagcggtg
      121 ttcggtgcga gccggtacga acagcatgtc gccaaccttg cacatatggc gctcgcggcc
      181 gctgcggaac caccccgaac cacgcgcgac tatgtatagc tcgtcgcgcg catgtggcgt
      241 ttgcaaatcc tcggccgcgg ggcaatacat cttgacttgc agggtaccgt gctcgaacac
      301 gactgccgat gggatgcctg cgcgaatatt ggctacggcg ccaagcgcgt cagcaatgct
      361 tgccgcgcgc ctgtttgcgg atgctgtgaa attcttttcc ggcttcatga gtcggcttat
      421 ttccccgtaa gctgtttcca ccggaagcag tctacggtgt gatcgttgac catgccgacg
      481 gcctgcatga aagcatacat gatggtgctg ccgacgaatt tgaaaccacg gcgcttgagg
      541 tctttgctca tggcgtcgga ctgagccgaa aacgctggca cctgccgggc agaggtccac
      601 gcattgcgtt gcgccttgcc ttgaacaaat tgccaaaggt acgcatccaa gctctggaat
      661 tcagcctgca ctgccaggca ggcccgcgca ttggcgactg cagattcaac cttcagccgg
      721 ttgcgcacga tgcccggatt ctgcagcaga cgttcgatcc ggccctgatc gtatttcgct
      781 atcttgcggg gatcgaaacc ggagaatgca acacgatagt tttcgcgttt gttcaggata
      841 gtggaccagg acaagccggc ttgagcgcct tcgaggatca taaattcgaa caatgtcgtg
      901 tcatcgtgct gaggcacacc ccattcgcga tcatggtatt cgagatagtt cggggtgccg
      961 ccggtggccc aatggcaccg ctgcatggtt gcctgcgatc tcatgccagt cgcgatcagc
     1021 tgccccgtga tcagactcgg gccggcatgc ggcggcgcca ttcgtggatc gcgagcccgg
     1081 tcccgaccag ataggccgct gcaattgcga tcagttgtgc atcgccaacc ggttttccgt
     1141 ataacaggct acccagcgca caggccaccg cggcatggct caatagcatg attgcctggc
     1201 tcgcataagc cgacagggat ggtttttcgc ccttcttgta aagggcgatg ccgcgcaagc
     1261 cggtcagggc agtcgataac agctgcacgg tcaatcccac ggcggcaagg atcgcgacgc
     1321 cggcagccgg tttagcccat tccggagcaa tcgccgatag cgctggtatc agtagataag
     1381 ccgccgcaaa ataaaatgtt aaaaggacga gcacgccgaa accaagcagg aaattgatac
     1441 gcgcaaacac gtgtgttgat actccggaca aggcgggatg ctcatgagac caaagccaca
     1501 gggctacgtc aattgaagtc ggaggctgat tttccagacg acaccgtaac ctttaccgga
     1561 aagcaccgca gaaattgcaa ccacggggct tctctggagc aggaatcaag cgcttagtgc
     1621 gttataccgg caatggcacg tggattgcgt caggcctcag ttccaaacca aaccacacaa
     1681 aggagactga acatggccag ttccaaagcc ccggcgaagt ccgggcggca ggatgcgacg
     1741 caaattcttg ccgctgacca caagaaggtc agcaaaattt ttgcagaatt cgaaaaaata
     1801 aaggaccgcg acagcgaagg caagcagcaa cttgtgaaga aggcttgtga cgagctaacc
     1861 gttcacgcgc aggtggagga agagattttt tatccggccc tgcatgaagc actgaaggaa
     1921 agcgaccagg acctggttga cgaagcggaa gtggagcatg cgtccatcaa acagctgatt
     1981 gaaacattga agacgtgcga tccagaggat cgccattacg acgccagcgt catggtcctt
     2041 tcggaatacg tgaagcacca tgtgaaggaa gagcaagacg agatctttcc caaggcgcgc
     2101 aaggccaagg acctcgactt gaaacaaatg ggcgaagaga tcatggcccg caagatgcag
     2161 ttgatggagg aacacggcat ggagatggaa gacatgccgg cctcaaggaa gcggcaaccc
     2221 gcatcccaac gacacgcgtg agatgcccgc tgtttcgcaa gcgatgtgcc cccggcgcca
     2281 gcggggtcgg ggaaatatgc agcccggcac ggccgtttcc gaaaaagaat cggccgtgca
     2341 atcggcgcgc aaggtcggcc tgcgctatgt aaccgacgcg aaaccgggaa tccggcggga
     2401 acgtctgcgc gggcgcttcc gctatatcga tccgtcaggg agaacaatca aagaccaaac
     2461 gacgctggcc cgcattaaag cgctggtcat tccgcccgcg tggagcgagg tctggatttg
     2521 ctctgatccc aatggccaca tgcaggttac cgcgcgtgat gcgagaggtc gcaagcagta
     2581 ccgttaccat gcgctctggc gcaaagcccg ggacgagaat aaatacgaac atctcttatc
     2641 gtttgcgaat gcgttgccgg cgattcgccg ccaggtcgac aaggatctag cccggccggg
     2701 cctgccacgc gcaaagattc tggcgaccat agtgcgattg ctcgaaacga cgatgatgcg
     2761 tgtgggcaac gaagaatacg cgcgcagcaa cgggtccttt ggactcacca cgcttcgcga
     2821 caagcatgtg cagatccacg gtcgccgtct tcagttccag ttccgcggca agagcggtgt
     2881 gcagcatttt gtcgagctga acgaccgtcg gctggcacgc atcgttcgtc gttgccggga
     2941 tttacccggg tacgagttat tccagtatct ggatgaggaa ggaaattgcc ggacgataga
     3001 ttcgggggac gtcaacgact atctgcgcga aatttcgggg tgcgactata cggcgaagga
     3061 tttccgcact tgggccggta cgctgctcgc cgcgatggcg ttacgcgaat tcgagacgtt
     3121 cgattcgcat gcgcaggcca agaaaaacat gatcagggcg attgaatccg tggcggaaaa
     3181 gctcggcaat acgccggccg tctgccgcaa gtgctacatt caccctgcca tcatggagtc
     3241 atacctggag ggctcgatgc ttgaaacctt gaagcatcgg gcggaagaga aattggtgca
     3301 tgagctgcaa gatctgaatg cggaggaggc ggcagtgctt gcgctattgc aacagcgatt
     3361 gcaacgggag caacggaaac cggccagcag gcggcacacc ggcgcgaaga cgcagccagc
     3421 ccggcgaccg gcgcgcgtgg cagagcgtcg gcttcaatcg ccaatatagt tggatacctc
     3481 tatcaggttc aggtccggat cgcgaaagta cactgaaagc agcgtcccga ccgccccggt
     3541 acgctttacc ggcccctcga tcagcgctac gccatgggcc tgcagatgcg ctgttacctg
     3601 ctgcatgggc accgaggtaa tcaggcagaa atcacctgac ccgggctttg gacagtccgc
     3661 tttcggctcg aattcctttc cttgctgatg caggtttatt ttctgcgagc caaatgccag
     3721 ggccttgcga cctgcgccga aatttacgat tttcatgcct aacgtgcccg cataaaaggc
     3781 gcaggtatta tcgatatccc gtactgtcag caccagatgg tcgatccgat ctatttccat
     3841 gatgttctcc gagccggagc aaagcgccag tgccggcaat gatagctgca ttgaggtaag
     3901 ctggcttcgc gggcgcagtg atatccggcc tgcgattaca gacgggagga tcgaatgaat
     3961 tcatcctgga caagacgcga ctttctgatt ggcgctggtg cagcgctggc cgcaacgcag
     4021 gcaatcgatc atttcgatcc gtgctgtgca atggcggcgg attcgaatac ggcagaggac
     4081 gaactattcg aattgaagcc ggttgcggat ggaatttatg cggcgattgc agcgccgcgc
     4141 tacaaggtca actgcaatgc cgcggtaatc gtgaccaatg acggcgtcgt ggtggtggac
     4201 tcgcactcca agccgtccgc tgcttttgcg ctctatcgag aaattcagtc agtcaccaga
     4261 caaccgatca agcgcattat caatacccat ttccactggg atcactggca gggcaatcag
     4321 gtttacgcac aggccttccc aggactggaa attattacgt ctgaacggac ccgtgacaac
     4381 ctgacacggc cgggcgcagg ttccggcggt gtgccctata tcgagaagca gctcgagctc
     4441 gtcccgaagg aaattgaaaa gctcggcaac gaactcgcat cggctaccga tcccgcgcag
     4501 gtcatgcggc tcagggcgaa cctgaggcaa accgaggcct atcttgagga acttcggcag
     4561 ctaaagccgg cgttgcctac ccgcacattc gatcagaccg tcagcctcaa cgttggcgaa
     4621 cgtgaaatcc agatcctgtt gctcggacgt ggccataccg atggcgacgt tttcatccac
     4681 ctcccaaaag aaaaagtggt tgcgactggc gacgcgctga ttgactggat gccgttcatg
     4741 aacgacggtt atccggagga ctgggtccgc accctcgacg cattggaaaa atttgaattc
     4801 acgaaaatta ttccgggcca tggcggggtg atgccccggg accacctgac attctttcgc
     4861 agctacctga ccgatctggt ggccgctatc aagaaagctc atggagatgg tgcaacgctc
     4921 gacgaaatga aacgctcact gcccgatcag ttggcgacca agtacgaagc cggcatgtcc
     4981 aagcatcctc ttggccagta tcgcgaccgt atcggtttga acatcgaagt cgcttacaac
     5041 aaggtcatca agaaagcctg ataaccaagg ctggacaaga tgcaggttga tgcaaagcgc
     5101 cagccgcggg agtgtggcgt tcggcgcccg tgcggctccc tcaatgaggg cttccccaaa
     5161 ccccacagga acgtttgccg ccgcaggcag tctgctttga cagtactttc cgcaaattga
     5221 tgcgcatgga tttggtcatc gtgcgtccgg aagggctgta ctgtccaccg ggcgattttc
     5281 atatcgatcc ctggcggccg gttgagcggg ccgtcattac gcatgcgcac ggcgaccatg
     5341 cgcgcccggg cagcgcgaaa tatttagcga ccgacgatag tgcttgcgta ttgcgcaaac
     5401 gtctcggtga tgccgccctg gatgtcgctc cctatggcga cagtttcaac ctgaacggcg
     5461 tcaaagtgtc gttccacccg gccggccatg tgctcggatc ggcccaggta cgaatcgaat
     5521 acggcggcga agtctgggtc gtctccgggg actataagct tgagggcgat accacctgcc
     5581 tggcattcga gccggtgcgc tgccatacct tcgttaccga atcaaccttc ggactgccga
     5641 tctatcgctg gatgccgcag aggcagatct t
//
LOCUS       DATMZQ010000228         5183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3282280, whole genome shotgun sequence.
ACCESSION   DATMZQ010000228 DATMZQ010000000
VERSION     DATMZQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282280"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1345)
                     /locus_tag="VN496_07440"
     CDS             complement(137..1345)
                     /locus_tag="VN496_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXF16821.1"
                     /translation="MAAYQYKAIDRTGRPARGKLDAANEVDLELRLKKMGLDLIIAKA
                     VRRQGGPSIARGSVTRQDLITFCFDLEQVSRAGIPLLDGLRDLRGGMENPRFREILTS
                     VLEDIEGGKTLSQALEGFPRVFNGVFVSLIRAGELSGTLPEVFENLGGTLKWQDELVS
                     QTRRLLVYPSLVFVVVTSVMIFLLLYLVPQVTQLLKTMGLELPIQTRALIWMSNFTEQ
                     WWPLLLFLPVVAAVVITFMVRTRPRARYLFDYALLRLPITGEILQKIILARFANFFAL
                     MYRSGITVLDAVKTSEDIVGNRVVADGLQRAGQQINSGESMSEAFQNLGMFPPLVIRM
                     LRVGESTGALDTALMNVTYFYNRDVRDSVDKALRLLEPTLTLVLGGMLGLILFSVLTP
                     VYDVIGKLKL"
     gene            complement(1374..3080)
                     /locus_tag="VN496_07445"
     CDS             complement(1374..3080)
                     /locus_tag="VN496_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXF16822.1"
                     /translation="MSEQRKKLRLGELLVQQGLITQDQLRIALTEQKAQNMPIGRLLV
                     RLGFVTEAVIRDIMARTIGQESIDLSQVLVDAEALKLVPQEFARRHRLLPIAYDAEQN
                     ILMVAITEVFNVVALDQLRATIGHNIQMKTVLAGQAQLDEFIDQFYGHELSVDGILRE
                     IETGEIDYQSLQQGGQDEYTQPIVRLVGSLLVDAVKRGSSDIHFEPEFAFLRIRYRID
                     GVLEQIRSLHKNYWPGIAVRIKVISGMNIAETRAPQDGRLSLNLNGRPVDFRVSSQPT
                     IWGENIVLRVLDREKSIISLDQMKLTKPVMSKLNLMLARPEGILVLTGPTGSGKTTTL
                     YSLLAKLNVESVNIMTLEDPVEYPVAMMRQTSVSEMAKIDFANGIRSIMRQDPDIILV
                     GEVRDRETAEMAFRAAMTGHQVFTTLHTNSALGAFPRLLDIGIAPDIMAGNIIGIIAQ
                     RLVRVLCIHCKEAYTPSVDERKLLDREVDSEFKVYRPVGCSRCNNRGYRGRTTVMEIL
                     HMDADLDELVARRASPREMREAALAKNFRSLAEEGIARVVEGSTSLAEVARAIDLTGR
                     AR"
     gene            complement(3090..4421)
                     /locus_tag="VN496_07450"
     CDS             complement(3090..4421)
                     /locus_tag="VN496_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF16823.1"
                     /translation="MSLLLQALQKAAKSRAEGDTEDSADALAVAQELALEPTSAGLHP
                     SNAEPASSPAQAATVVQAGRVPAFDPVDYAREHYMVTFIGVALLFAVGYAAYVYMQLN
                     RPFRNAAPAPFAAAPAPQVPVANAPPLSASAKISGMPGAGPTDDGARAQKVATVPESD
                     AAPPGAEPSTASAALSRDSAGLRAEKRYARTGAAKQAAPTLAAAPPMSRQGNIEPVAL
                     ADASDTIETVVIPASRSKSSGDISVHRDAAGITPVSPTLMQAYETLQRGDISRAKELY
                     GQVLQADPRNIDALLGLASIALTDSRTDDASRYYQSVLDLDPRNSYAQAGLISIIGSA
                     DVQASEARLKQLIARDPSAFLYFSLGNLYAEQGQWPAAQQAYYQAFQMQQDNPDFAFN
                     LAIGLEHIGQNRLALDYYRKALDLSFKKGHANFDQSLVIQRVGQLSTRIEQ"
     gene            complement(4418..>5183)
                     /locus_tag="VN496_07455"
     CDS             complement(4418..>5183)
                     /locus_tag="VN496_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HXF16824.1"
                     /translation="GMSFTQQLLGAPPTGAPNGLTVGYTNPNSSVGNVSATVRLLEQF
                     GNTRILSSPKLMALNNQAALLKVVNNVVYFFIEAQTNTNQTVSTTTFTSTAKTVPVGL
                     IMSVLPQITEHGTVNLTVRPTISRVTRFVSDPNPSLQVDSKGVQLTNPIANLVPEILT
                     REMESVLQATSGQTIILGGLMQDSVRRDRDAVPGLGKLPSPVGDIFSFRDEAVLKTEL
                     IIFIKPTVISNPSLDSDELKFFRRFLPVIDPTGKTP"
BASE COUNT         1140 a         1439 c         1443 g         1161 t
ORIGIN      
        1 aaggaagttg ctgaacgcgt tccagccagc ctggtcgttt tcaaacctct tgcacgatgc
       61 caggcggcga cctcgccgta cggcggcggt tgcctgatca ttggatacgg aaagcaatag
      121 ttttgacgac atggcgctat agcttgagct tgccaatcac gtcgtacacc ggcgtcaata
      181 ccgagaacag gatcagcccc agcatgccgc caagcaccag cgtgagcgta ggttccaata
      241 accgcaacgc tttatccacc gaatcgcgga catcacgatt gtaaaagtaa gttacgttca
      301 ttagtgcggt atcaagcgca ccggtgcttt ccccaacccg gagcatgcga attacgagcg
      361 gcggaaacat gccgagattt tgaaaagctt ccgacatgct ttcgccagaa tttatctgtt
      421 gaccggcgcg ctgcaggccg tctgcgacaa cccggttgcc cacaatatct tcgctggttt
      481 tgaccgcgtc cagcacggta atgccggaac ggtacatcag cgcgaagaaa ttggcgaagc
      541 gcgcaagaat gattttttgc agtatttcac ctgtaatcgg caagcgcagg agagcgtagt
      601 caaagaggta gcgggcgcga ggcctggtac gaaccatgaa cgtgatgaca acggccgcta
      661 caacggggag gaacaaaagc agcggccacc attgctccgt aaagttcgac atccatataa
      721 gagcgcgtgt ctggattggc aattccagcc ccattgtttt caaaagttgc gtgacttgcg
      781 gtaccagata caacaataaa aatatcatca cgcttgtcac gaccacgaat actaaagagg
      841 gatagaccag cagccttctc gtctgggaaa ccaactcatc ctgccatttc agggtgccgc
      901 cgaggttctc gaatacttcg ggcaacgtcc cggaaagttc gcccgcccgg atcaagctta
      961 caaagacccc attgaacacc cgcggaaagc cttccagtgc ttgtgacaag gtcttgccac
     1021 cctcgatatc ttcgagtacc gaggtcagga tttcgcggaa tcggggattt tccattccgc
     1081 ctcgcaaatc cctcaaacca tcgagcaacg gtatcccggc gcgggagact tgttcgagat
     1141 cgaaacaaaa cgtaatcagg tcctggcgcg tgacgctccc gcgggcgatg gatggccccc
     1201 cttgcctgcg caccgccttg gcaatgatga gatccaggcc catctttttg aggcgcaact
     1261 caagatcaac ctcgttcgcg gcatcaagtt tgccgcgtgc cgggcgtcct gttcgatcga
     1321 ttgccttgta ctggtatgct gccatgggtg cctatgcctg tgccgtttcc tcgctatcgc
     1381 gcgcgacctg tcagatcaat ggcgcgcgcg acttccgcga ggcttgtgga gccctccact
     1441 acgcgcgcga tgccctcttc agccagagaa cgaaagtttt tagccaaagc agcttcacgc
     1501 atttctctgg ggcttgcgcg ccgtgcgacc agttcgtcca ggtccgcgtc catgtgcaaa
     1561 atctccatga ccgtggtacg cccgcgatat ccgcggttgt tgcaacgact gcatccgact
     1621 ggccggtaga ccttaaactc gctgtccact tcgcggtcca gaagttttcg ctcgtctaca
     1681 gacggcgtat acgcctcctt gcaatgaata cacaacacgc ggaccaatcg ctgcgcaatg
     1741 atgccaatga tgttgccggc catgatgtcc ggcgcgatgc cgatatccag caatcgtggg
     1801 aaggcgccca atgcagaatt ggtgtggagc gtggtaaaaa cctggtgtcc cgtcatggcg
     1861 gcgcgaaatg ccatctccgc agtttcacga tcgcgaactt ccccgaccag aataatgtcg
     1921 gggtcctggc gcatgatcga tcggatacca tttgcgaaat cgatctttgc catctccgac
     1981 actgacgtct gtcgcatcat tgcaaccgga tattcgaccg ggtcctcgag cgtcatgatg
     2041 ttgaccgact caacattgag cttggcaagc aatgaataca gggttgttgt tttgccgctc
     2101 ccggtcggtc cagtgagtac caggatgcct tctgggcggg caagcatcag gttgagtttc
     2161 gacattaccg gcttggttaa cttcatctgg tccaggctga taatcgattt ttcacgatcc
     2221 agtacgcgca gcacaatgtt ttcaccccag atggtcggct gcgacgatac tcggaaatcg
     2281 actggccggc cgttcaaatt gagtgaaaga cgcccgtctt gcggcgcgcg cgtctcagca
     2341 atgttcattc cgcttatgac ttttattcgt acggcaatgc ctggccaata gttcttgtgc
     2401 agactgcgga tttgttccag gacgccgtcg atgcgatagc gaatgcgcaa aaaagcgaat
     2461 tcgggttcga agtggatgtc ggacgagcct cgcttcacgg catctaccaa caaggaaccg
     2521 accaaacgca cgatcggctg ggtgtactcg tcctgaccac cttgttgcag actttgatag
     2581 tcgatttcgc cggtttctat ttctctcagg atgccgtcaa cggaaagctc atgcccatag
     2641 aactggtcga taaactcgtc gagttgcgcc tgtcctgcaa gcactgtctt catctggatg
     2701 ttgtgcccaa tcgttgcgcg caactggtcc agggcaacta cattgaagac ttcagtgatt
     2761 gcgaccatca gaatattttg ctcagcgtca taggcaatgg gcagcaggcg gtggcgacgt
     2821 gcaaattcct gcgggacgag ttttagagct tctgcatcta ccaaaacctg ggaaagatcg
     2881 atgctttcct gaccaattgt ccgcgccatg atgtcgcgga ttacagcttc cgtaacaaag
     2941 cccagcctca cgagcaggcg cccgataggc atgttttgcg ccttctgctc ggtaagcgcg
     3001 atgcgcaact ggtcttgtgt aataaggccc tgttgaacca gcaactcgcc caaacgaagc
     3061 tttttgcgct gctcggacat cgcgctagat cactgctcta tacgggtgga cagctggccg
     3121 acgcgctgaa taaccaggct ttgatcgaaa ttggcatggc cttttttgaa tgacaggtca
     3181 agggccttgc ggtagtaatc aagcgcaagg cgattctgcc cgatgtgttc aaggccgatg
     3241 gcgagattga atgcaaaatc gggattgtcc tgttgcattt ggaacgcctg atagtaggcc
     3301 tgttgcgcgg caggccactg gccctgctcg gcatacaaat taccaagcga aaagtaaagg
     3361 aaagcggacg gatcacgcgc gatcaattgc ttgagccgcg cctcgctggc ctgcacatcc
     3421 gcactcccga taattgaaat gagccccgct tgcgcataag aattgcgcgg gtcaaggtcc
     3481 aaaactgact ggtagtagcg gctggcgtca tcagtgcgac tatcagtcaa agcaatcgag
     3541 gccaatccga gcagtgcatc aatattgcgc ggatcggctt gcagcacctg cccgtacagt
     3601 tctttggcac gcgaaatgtc cccgcgctgc aaagtctcat atgcctgcat gagcgtggga
     3661 ctcacgggtg ttataccggc ggcgtcgcgg tgcaccgaaa tgtcgcccga ggatttcgac
     3721 cggctcgccg gaattacaac tgtctcaatg gtgtcgctcg catcggcaag cgcaacgggc
     3781 tcaatattcc cctgtcgcga cattggcggc gcagcggcaa gagtgggtgc agcctgtttc
     3841 gccgctcctg ttctggcata ccgcttttcg gctcgaagcc cggcagaatc gcgtgatagc
     3901 gccgcggatg cggttgaggg ctcagcgcct ggcggcgcgg catcgctttc aggaaccgtg
     3961 gcgacctttt gtgctcttgc cccgtcatca gtcggccctg cgcccggcat tccgcttatt
     4021 tttgcgctgg cggacaaagg cggtgcattt gcaaccggaa cctgcggcgc aggtgcagct
     4081 gcaaacggag caggggccgc attgcgaaac ggcctgttta gctgcatata gacataagcc
     4141 gcgtagccca cggcaaataa caaggcaacg ccgataaacg tcaccatgta gtgttcgcgc
     4201 gcgtaatcga ccgggtcgaa agccggcacc cttccggctt gcactaccgt tgccgcttgt
     4261 gccggcgatg acgcgggttc ggcattggaa ggatgcaaac ccgccgaggt cggttcaagc
     4321 gccaactctt gggcaacggc cagggcgtcg gccgaatctt ccgtgtcgcc ttctgcgcgg
     4381 cttttagcag ctttctgcag cgcttgtagc aacaagctca tggtgttttg ccggtgggat
     4441 cgattaccgg caagaaacgg cggaaaaact tgagctcatc actatccagc gacgggttgc
     4501 tgatgactgt gggcttgata aagatgatca gttcagtttt caggaccgcc tcatcgcgga
     4561 aactgaatat atcgccaacc ggggagggta gtttcccgag tccaggaacg gcatcgcgat
     4621 cacggcgcac gctgtcctgc atcagtccgc caagaataat ggtctggccg ctggtggctt
     4681 gcaggacgga ttccatttct cgcgtcagga tctccggaac caggtttgct atcggattag
     4741 ttaactgaac gcccttgctg tcgacttgca gcgagggatt gggatcagaa acaaaccggg
     4801 tcacgcgcga aattgtcggc cgcacggtta gatttactgt tccgtgctcg gttatctgag
     4861 gcaagaccga catgatcaac cctaccggca cggtcttggc ggttgaggtg aatgtggtag
     4921 tagaaaccgt ttgattggta ttggtttggg cttcgataaa gaagtagacg acgttgttga
     4981 ccactttcag tagcgccgcc tgattattca gcgccatcaa cttggggctt gaaagtatac
     5041 gtgtattgcc aaattgctcg agcagtcgca cagttgccga aacgttaccg acgctcgagt
     5101 tgggattggt gtagccgacc gtcagaccgt tcggcgcgcc cgtcggagga gcgccaagca
     5161 gctgctgcgt gaaggacatt cca
//
LOCUS       DATMZQ010000229         3733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1212151, whole genome shotgun sequence.
ACCESSION   DATMZQ010000229 DATMZQ010000000
VERSION     DATMZQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212151"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2955
                     /locus_tag="VN496_07460"
     CDS             136..2955
                     /locus_tag="VN496_07460"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXF16825.1"
                     /translation="MELAKSFEPTDIERRWYPVWESRGYFEAGKDEGRAPYCIQLPPP
                     NVTGTLHMGHAFQQTLMDALIRYHRMRGFATNWVIGTDHAGIATQIVVERQLEIEGKT
                     RHDLGREKFVERVWEWKQQSGSTITRQMRRLGASGNWTYADTEGQKGGYFTMDAKMSR
                     AVIEVFVRMHEEGLIYRGKRLVNWDPVLGTAVSDLEVDSEEEDGRIWEIRYPLAQGNG
                     GLVVATTRPETMLGDVAVAVHPVDERYRKLVGKQLQLPLGGRTIPIVADEYVDPEFGT
                     GCVKITPAHDFNDYAIGTRHGLPLINIMTLDARINDQAPAAYRGLDRFEARKRVLADL
                     RALDLVVSEKPHKLKVPRSGRTGVIVEPMLTDQWFVKMEGLAQRGLKAVATGDIKFYP
                     EHWTATYNHWLENIQDWCISRQLWWGHQIPAWYGEDGSIHVGRNEEEAKKLAAARGYT
                     GALKRDEDVLDTWFSSALVPFTSLGWPEKTRDLDLFLPSSVLVTGFDIIFFWVARMIM
                     MTLQFTGKVPFRDVYINAIVRDAEGQKMSKSKGNTIDPLDLIDGIALEDLIAKSTVGL
                     LRAEHKQKIEKAIRKNYPQGIPGYGADAMRFTFASLATFARTLNFDLNRCEGYRNFCN
                     KLWNASRFVLMNCEGKDCGLEEHTAEQCGPGGYLDFSQTDRWIVSLLQRTEAEVEKGF
                     AGYRFDNAASAIYKFVWDEYCDWYVEIAKVQMQSGTEAQQRATRRTLLRVLEVTLRLA
                     HPIIPFITEELWQKVAPLAGRHPGGDVSIMLQPYPKSQPAKIDEQSEAWVAQLKVLVE
                     ACRTLRGEMGISPAQRVPLLATGNASTLAAYFPYMQALARLAQTTVVDALPDTDAPTA
                     LAGETRLMLEIKIDVAAEIVRIEKEVSRLESEIAKARGKLSNAGFVQRAPAAVVAQEQ
                     ERLASFEAMVEKLHEQLAKLKRKAA"
     gene            2982..>3733
                     /locus_tag="VN496_07465"
     CDS             2982..>3733
                     /locus_tag="VN496_07465"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF16826.1"
                     /translation="MPALRAFKHRNFRLFYTGQAISLIGTWIQQIALAWLVYRTTGSG
                     FLLGLVTFCTQIPMLIFLPFAGLLSDRQDRRKLMITAYILAAVQASTLGVLTLVGDIR
                     IWQILLLGFLYGAIMAFETPARQSLISQMVNSRADLPNAIALNSVLMNSGRLIGPSIA
                     GMLLVFISEGWCFLINALSFAAIIASVSMMRLAPRTAPAIRGSLLQDLRAGARYAWHT
                     RPIRLFLALVALISLTASPYTVLMPIFARDVFG"
BASE COUNT          776 a         1092 c         1136 g          729 t
ORIGIN      
        1 agcggctggt cgccgagctc aaacgcgcgc tgccggccgc ggtacgtgcc gcgatcaccg
       61 ataagacatc cgatccagcg gtgttccgca acgacagtcg tctataattt cccgcctagt
      121 ttttgccgct gaaaaatgga acttgcaaag agctttgagc cgaccgacat cgagcggcgc
      181 tggtatccgg tctgggaatc gcgcggctac ttcgaggcag gaaaggacga ggggagggcg
      241 ccatactgca ttcagcttcc gccccccaat gtcaccggca ccctgcacat gggtcatgct
      301 ttccagcaga cgctcatgga tgccttgatc cgttatcacc gcatgcgggg cttcgccacc
      361 aactgggtca ttggaacgga tcacgccggc atcgcgactc aaatcgtggt cgagcgccaa
      421 ctggagatcg agggcaagac ccggcacgat ctcgggcggg aaaaattcgt cgaacgcgtc
      481 tgggaatgga aacagcaatc gggttccacg attacccgtc aaatgcgccg gctcggtgct
      541 tcgggcaact ggacctatgc cgacaccgag gggcagaagg gcggctactt cacgatggac
      601 gcgaagatgt cgcgcgcggt catcgaagta tttgtgcgta tgcacgagga agggctgatc
      661 taccgcggca agcggctggt gaattgggac cccgtcctgg gcacggcagt ctccgacctg
      721 gaagtcgaca gcgaagagga agacggccga atctgggaaa tccgctatcc gctcgcgcaa
      781 ggcaacggag gtttggtcgt cgccaccacg cggccggaaa ccatgcttgg cgatgtcgcc
      841 gtcgcagttc atccagtaga tgagcgttat cgaaagctgg tgggaaaaca actgcaattg
      901 ccgcttggcg gacgaaccat tccgatcgtc gccgacgaat acgtcgatcc ggaattcggc
      961 accggttgcg taaagattac gccagcgcac gatttcaatg actacgccat cggcacccgg
     1021 cacgggcttc ccctgatcaa catcatgacg ctggatgcca gaatcaatga ccaggcgccg
     1081 gccgcgtacc gcgggctcga cagattcgaa gcccgcaagc gcgtcctcgc ggatttgcgc
     1141 gcactggacc ttgtcgtttc ggaaaagccg cacaaactga aggtgccgcg ctctggacgt
     1201 accggcgtga tcgtcgagcc gatgctgacc gatcagtggt tcgtgaaaat ggaggggctc
     1261 gcgcagcgtg gcttgaaagc ggtggcgacg ggcgatatca agttctatcc ggagcactgg
     1321 accgctacct acaaccactg gctggaaaac attcaggact ggtgcatctc ccgccagctc
     1381 tggtggggcc atcagattcc cgcatggtac ggcgaagacg gctcaatcca tgtcggccgc
     1441 aatgaggaag aagcgaagaa actcgcagcc gccaggggct ataccggcgc gctcaagcgc
     1501 gatgaagatg tgctcgacac ctggttctcc tctgcgctgg tgccgttcac ctcgctcggc
     1561 tggcccgaaa aaacccgcga cttggatttg ttcctgcctt catcggtcct ggtcacgggg
     1621 ttcgacatca ttttcttctg ggtcgcgcgc atgatcatga tgacgctgca gttcaccggc
     1681 aaagtgcctt tccgtgacgt gtacatcaat gccatcgtgc gcgacgccga gggacagaag
     1741 atgtcgaaat cgaagggcaa taccatcgat ccgctggacc tgatcgacgg tatcgcgctc
     1801 gaagacctga ttgcgaagtc cacggtcggg ctactgcggg ccgaacacaa acagaagatc
     1861 gaaaaggcca ttcgcaagaa ctatccgcaa ggcattccgg gttacggtgc cgacgccatg
     1921 cgcttcactt tcgcctcgct ggcgacgttt gcgcgaaccc tgaacttcga tctgaaccgt
     1981 tgcgagggct accgcaattt ctgcaacaaa ctgtggaacg ccagccggtt cgtgctgatg
     2041 aactgcgaag gcaaggattg cgggctggaa gaacacacgg ccgagcaatg tggtccgggc
     2101 ggttatctgg atttttcgca gactgaccgc tggattgtca gcctgctgca gagaaccgag
     2161 gcggaggtgg aaaagggatt tgccggctac cgcttcgaca atgccgccag tgcgatctac
     2221 aagttcgtct gggatgagta ctgcgattgg tatgtggaaa tcgccaaagt acagatgcag
     2281 tccggcactg aagcacaaca acgcgcgacc cggcgcacgc tgctgcgagt ccttgaagtg
     2341 acgctgcgtc tcgcacaccc gatcattcct tttatcaccg aggaactgtg gcagaaggtc
     2401 gcgccgctgg ccggacgcca tcccggcggg gacgtttcga tcatgttgca gccctacccg
     2461 aagtcacaac ccgcgaaaat cgatgaacag tcggaagcct gggtcgcgca actcaaggtg
     2521 ctggtggaag cgtgcaggac gctgcggggt gaaatgggga tttccccggc acagcgcgtc
     2581 ccgctgctgg caaccggcaa cgcatcgacc ctggctgcct actttcccta tatgcaagcg
     2641 ctggcaaggc tggcccaaac gacggttgtc gatgcccttc ctgacaccga cgctccgacc
     2701 gctttggcag gcgagacacg gctaatgctg gaaatcaaga tcgacgtggc cgccgagatc
     2761 gtccgcatcg agaaggaggt ttccaggctg gaaagtgaaa tcgccaaggc gagaggcaaa
     2821 ctttccaacg cgggattcgt gcagcgcgca ccggctgccg tagtcgccca ggagcaggaa
     2881 cggctggcgt ctttcgaggc catggtcgag aaactgcacg agcaactcgc caagctgaaa
     2941 cgaaaggcgg cctgacccgg gtgttgcggc gcctgaccgg catgcccgca ctgcgggcat
     3001 tcaaacatcg caatttccgg ttgttctata ccggtcaggc gatttccctg ataggtacct
     3061 ggatacagca gatcgccttg gcctggctgg tttatcgcac caccggttcc ggtttcttgc
     3121 tgggactggt gacgttctgt acgcagattc ccatgctgat tttcctgcca ttcgccggtc
     3181 ttctgagcga tcgacaggat cggcgcaaac tgatgatcac tgcgtacatc ctggcagcgg
     3241 tgcaggcatc gacgctgggc gtactcacgc tcgtgggtga catccgcatc tggcagatcc
     3301 tgctgctggg atttctctat ggcgcgatca tggctttcga aacgccggcg cggcagtctc
     3361 tgatcagcca gatggtgaac tcgcgcgccg acctgccgaa cgcgattgcc ctgaattccg
     3421 tactgatgaa cagcggacgt ttgatcggcc cgtccatcgc cggcatgttg ctggtgttca
     3481 tcagcgaagg atggtgtttc ctgatcaatg cgctgagctt tgcggcgatc atcgcgtcgg
     3541 tatccatgat gcggctggcg ccaagaactg cgccggccat acggggatca ctcctgcagg
     3601 acctgcgcgc cggggcacgc tatgcctggc atacacggcc gatccggctg ttcctggcgc
     3661 tcgttgcatt gatcagcctg acggcttcgc cttacacggt actgatgccg atattcgcgc
     3721 gcgatgtgtt cgg
//
LOCUS       DATMZQ010000230        20012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1472382, whole genome shotgun sequence.
ACCESSION   DATMZQ010000230 DATMZQ010000000
VERSION     DATMZQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472382"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VN496_07470"
     CDS             complement(<1..310)
                     /locus_tag="VN496_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXF16827.1"
                     /translation="MKKPVSGLYAITPELTDSIALFQKVRAALRGGAQVIQYRAKSIP
                     DSLRRAQAEEIADSCRSHNALFIVNDSIALAQAVRADGIHIGELDADVTLAREALGPG
                     K"
     gene            complement(294..1130)
                     /gene="thiD"
                     /locus_tag="VN496_07475"
     CDS             complement(294..1130)
                     /gene="thiD"
                     /locus_tag="VN496_07475"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXF16828.1"
                     /translation="MPASPPIVLVFSGTDPSGGAGMQADVLTLSSMGCHPLSVVTAVT
                     IQDTMGVEDVLPIDAEWVADQARCVLEDVPVAVFKIGLLGSVDTVAAIAEVVSDYPDI
                     PLVLDPVLASGRGDELASQELILAMRELLIPQTTLITPNSLEARRLMQEDDADDEDNP
                     DLGECANRILGLGCEYVLITGTHENTPQVVNTLYEQQGVVRADSWQRLAGTFHGSGCT
                     LASAIAATIANGLSVPEAVRDAQEYTWQTLKHAYRTGMGQLVPDRLFWAREEEPEDEK
                     AR"
     gene            1192..1380
                     /locus_tag="VN496_07480"
     CDS             1192..1380
                     /locus_tag="VN496_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXF16829.1"
                     /translation="MSASGEQTYKTFMCLICGFIYDEAEGLPEEGLPAGTRWEDVPVN
                     WVCPECGARKEDFELVEI"
     gene            1395..1781
                     /gene="gloA"
                     /locus_tag="VN496_07485"
     CDS             1395..1781
                     /gene="gloA"
                     /locus_tag="VN496_07485"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HXF16830.1"
                     /translation="MRILHTMLRVGDLKRSVDFYTNVLGMQVLRQKDYPDGRFTLAFV
                     GYGTEDKNSVLELTHNWDTPRYDLGNGYGHIALEVNDAAKACDDVRQRGGKVTREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQRKA"
     gene            complement(1795..2559)
                     /gene="rsmA"
                     /locus_tag="VN496_07490"
     CDS             complement(1795..2559)
                     /gene="rsmA"
                     /locus_tag="VN496_07490"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXF16831.1"
                     /translation="MHRPRKRFGQHFLEDRAVVGQMVAAISPNSAQRLIEIGPGLGAL
                     TVPLLERVGHLDVIEIDRDLVQQLRDRYPVNRLTVHDVDALEIDLAQFGRDLRVVGNL
                     PYNISTPLLFHLTDHASCVRDAHFMLQREVVQRMSAQAGSSAYGRLSVMLQYRWQIDA
                     LFDVSPTAFRPAPKVWSSVVRMIPHTVLPYVARDEKVFADVVMRAFGQRRKTLRNTLR
                     GKLDASDFAKLEIDPAARGESLAVRDFVNIANRIAS"
     gene            complement(2636..3946)
                     /locus_tag="VN496_07495"
     CDS             complement(2636..3946)
                     /locus_tag="VN496_07495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXF16832.1"
                     /translation="MKRSLYAAVLIACIALPVGAAQEERKKYDAIALDRVVAVVNSEV
                     VTRLDLDEQVKVALMQLKRQGTPLPAREVLERQLLDRLVTSKVLAQSAKETGLRVDDT
                     QLQRAIERIAQENKVSPEQFRKTMESEGIDFNRFREELRNEILIARLKEREVDSKILI
                     TDAEIDNYLKNEQSQSGKDEEYSLAHILIMVPEQASPEQIKSKRVIAEKALDQIKGGA
                     DFRQVSAGVSDAQNALDGGPLGWRTASRLPQIFVESVKSMNVGDVSPVLRSANGFHIL
                     KLVDKRGNESPVIIQQTHARHILIRLNEVVSEAEAKQRLNNLKERIENDTSFAELARL
                     QSEDATASRGGDLGWLSPGDAVPEFERAMNALQPGQVSDPVQTPFGWHLIQVIERRNE
                     DMSKERQRVLARQSIRTRKADEAYQEWVRQQRDRAYVELRLENH"
     gene            complement(4014..6230)
                     /locus_tag="VN496_07500"
     CDS             complement(4014..6230)
                     /locus_tag="VN496_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HXF16833.1"
                     /translation="MHHSRITSAVLSLILYVALDVHAENPGLKLRMQPDLIPYSLGRD
                     EPSTLFIEADKIEGNQEQELDASGNVRVRKRGTAVFSDQVHLNIPEEQVTATGHVRFE
                     KEGDIVTGELLFYDLNYESGNIDKPTYQLTRYGARGNADRLVAENPNLTRIDKATYTT
                     CDVGDDDWLMRINRLELDRISDIGVARNATVVFKGVPILYSPYLDFSLSGRRKTGLLP
                     PTIGSTGSSGFEFTQPFYWNIAPNQDATISPRILSKRGVLMNGDFRYLEPTLKGEARA
                     EYLPDDRQKGESRYAYLWQHQQNFGNGFSGAVDVQGVSDDTYFTDLSDKIAATSQTNL
                     NREGDLFYDGGWWNVNTRVQRYQTLQDPLAPVVPPYARVPQITLNMNRSTDIRLDLGL
                     QGEFVHFSHPTLLNATREILYPSVSAPIQMPFFYITPKAGFSTTSYSFQDATTPDATR
                     NLPIYSLDSGLTFERDTTVWGRAFSQTLEPRLYYVYIPFRPQDQLPIFDTAQADLNFT
                     QIFTENQFSGGDRINDANQLTAAVTSRLIDPGSGDEQMRFMLGQRYYFKPQEVSLTPE
                     VRNSSRSDVLAGVTGQMTKYWFADVNTQYSTVASQTQRTDAVLRYRPDFGKMVNLGYR
                     YTRGSLEQADFSTEWPIGGRWTGLVRYNYSLQDRNLLEGLLGLEYNAGCWAARVVMHR
                     FVSATQEYVNAMFFQLELRGVSQIGSSPLEVLRQNITGYQKTTDQRTSESNPFPAF"
     gene            6326..7288
                     /locus_tag="VN496_07505"
     CDS             6326..7288
                     /locus_tag="VN496_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXF16834.1"
                     /translation="MSRQFEGQSFSLDPASSDASFRRYFRVTLRDATFIVMDAPPDRE
                     DCRPFLRVARLMSSAGLHVPAILAQDLELGFLLLSDLGSTTYLAALSNGNADELYRDA
                     TDALILWQRASVEGVLPLYDEALLQRELDLFPEWYLRRHLGLELDAQQKRSLAGVFKL
                     IIANNISQPRSYVHRDYMPRNLMLSTPNPGVLDFQDAVYGPITYDVASLFKDAFISWD
                     EGRVIDWTIRYWERARSAGLPVRSDFGEFYRDCEWMGLQRHLKVLGIFARICHRDGKP
                     AYVKDTPRFVAYVRAVCERYASLSPLLRLLDQIAEREPVSGQTR"
     gene            7320..8000
                     /locus_tag="VN496_07510"
     CDS             7320..8000
                     /locus_tag="VN496_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXF16835.1"
                     /translation="MILAAGRGERMRPLTDRIPKCLLKVDGKPLIFRHLEKLAEAGFG
                     TVVINHAHLGAQIEEAVGDGRRWNIRIRFSPEPQALETAGGILNALPLLHEPVFAVVN
                     ADVYSTYDYGNLVLGIGRMQATTLAHLVLVPNPPHHRDGDFSLEGNQVRMGTKERLTF
                     SGIGVYRATLFDQLVPGSRHPLAPLLVKQMALGRISGEYFRGEWDDIGTPQRLAQLEA
                     RLARTAEH"
     gene            8060..9361
                     /locus_tag="VN496_07515"
     CDS             8060..9361
                     /locus_tag="VN496_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HXF16836.1"
                     /translation="MIDPALYVRRRAQLAALMQEGVAVVRTAPERPRNRDSSFPFRFD
                     SYFHYLTGFPEPEAVLVLLAESPVKSILFCRDKDTEREIWDGFRFGPDQARETFGFDE
                     AHPITKLDELLPKLLANRSTVFCDVGADSSWDVQIIGWINKVRAQVRAGVSAPSEIRD
                     IRRFLDDMRLIKDEHEIGIMRRAANISSEAHRRAMRATHAGLHEYELEAELIHEFRRQ
                     GAQAPAYTPIVAGGGNSCVLHYVQNDGLLKDGDVVLIDAGCELDGYASDITRTFPVSG
                     IFSGPQRDIYQLVLAAQAAAIAQVKPGNDWEAPHDAAVRVLARGMIDLGLCKGTTDSV
                     IESGDYRRFYMHRTGHWLGMDVHDAGDYKRNGAWLKLQPGMVLTVEPGCYVRPGDNVP
                     EQFWNIGVRIEDDALVTEFGSEIISSAAPKTIEQIESLMNH"
     gene            9411..10580
                     /locus_tag="VN496_07520"
     CDS             9411..10580
                     /locus_tag="VN496_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXF16837.1"
                     /translation="MLSKTDYDIVIVGGGPVGAGVTIALQASGFSVLLLESRIDNSNS
                     DDQRTLALSFGSRLILERLGLWSQIGPATPIQSIHVSQRGGFGRTLLLAQELELPALG
                     YVIRYSLLQQTVNRALKSLAANLMTGAQAVSMDSTSQAIEIGIQTQGSRQAISARLLV
                     IADGGAGLGLQSGATTKVQEYGQDAVVGLVGTSQPHANRAFERFTPDGPIALLPFQNG
                     YALVWTAAPETADALLALPASTFLDKLQLHFGDRAGRFSSIAARARFPLTLRYAADPV
                     MPRAVMLGNAAQSLHPIAGQGFNLGLRDAWELSEVLLLHGKADPGSSACLSRYRAGRR
                     PDRLRSIGLTHSLVKIFSNDVLPLRAARGAALSLLDLVPSGKRAFMQRMIFGALR"
     gene            10655..11665
                     /gene="dusB"
                     /locus_tag="VN496_07525"
     CDS             10655..11665
                     /gene="dusB"
                     /locus_tag="VN496_07525"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXF16838.1"
                     /translation="MQIGPHALRNNLIVAPMAGVTDRPFRQLCKAMGAGMAVSEMISS
                     NAQLRDTVKSMRRSNHEGEADPISVQIAGADPGAMAEAARYNTDRGAQIIDINMGCPA
                     KKVCNVAAGSALLQNEPLVGKILDAVVAAVNIPVTLKIRTGWSPHNRNAVRVAQIAQS
                     AGVKALSVHGRTRACAFLGHAEYETIAEVKSRVAIPVIANGDIDSPEKAREVLRLTGA
                     DGLMIGRAAQGRPWIFREIQHYLINDKHLPPPDIAEIKELLVNHLHAVYELYGEQSGV
                     RVARKHIGWYVKGLAGASLFRQSMNKMEMTHQQLNAVEAFLDGLATRGDRLTYSEEEL
                     AA"
     gene            11665..11898
                     /locus_tag="VN496_07530"
     CDS             11665..11898
                     /locus_tag="VN496_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXF16839.1"
                     /translation="MNDNEIARCVRRAMDGYFKDLEGERPCAVYDMVMNSVEKPLLEI
                     VLHRVSGNQTQAAQLLGMNRNTLRKKMKYYGIK"
     gene            11966..13525
                     /gene="purH"
                     /locus_tag="VN496_07535"
     CDS             11966..13525
                     /gene="purH"
                     /locus_tag="VN496_07535"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXF16840.1"
                     /translation="MNRRKALVSVSDKSGAADFARFLHEIGMEILSTGGTAKLLREAN
                     VPVSEIGDYTGFPEMLDGRVKTLHPKVHAGILARRDLPEHLNATARAAIPLIDLVVVN
                     LYPFAQTVSRAGCTLEEAVENIDIGGPTMVRAAAKNWQHVAIVTDPADYPALIAEMKS
                     ASGAISGETRFWLAKKAFSHTAAYDAAISNYLTATDASGARVEFPDQINLNYAKVQDL
                     RYGENPHQQAAFYRELKRVPGALSNYRQLQGKELSFNNIGDADAAWECVKTFAEPACV
                     IVKHANPCGVAVSVNCLEAYSRAFKTDPTSAFGGIIAFNRQIDVETAEALTQQFMEVL
                     IAPGIADDAARVLARKTNVRVLEIASEAGANRFDFKRVGGGMLVQTPDTMNVQLNDLR
                     IVTKKQPSSGQLADLMFAWRVAKFVKSNAIVFCGDGMTLGVGAGQMSRVDSARIATIK
                     AENAGLSLAGSVVASDAFFPFRDGVDVVAKAGAIAIIQPGGSMRDEEVIAAADEHGII
                     MALTGVRHFRH"
     gene            13572..14855
                     /gene="purD"
                     /locus_tag="VN496_07540"
     CDS             13572..14855
                     /gene="purD"
                     /locus_tag="VN496_07540"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXF16841.1"
                     /translation="MNLLVIGSGGREHALAWRLAQSPRIGRIYVAPGNAGTAQEDGLF
                     NVPLTAIPDLLDFARKEAVALTVVGPEAPLAAGIVDTFRSAGLRIFGPTRAAAQLEAS
                     KDFAKAFMVRHGIPTAAYASFTDAKAAHDYVAQRGAPIVIKADGLAAGKGVVVATTPE
                     EAHAAIDLMLVSNKMGVAGARVVIEEFLEGEEASFIVMVDGKHVLPLASSQDHKRLLD
                     ADKGPNTGGMGAYSPAPVLTPAIHARVMREVIQPVIQGMEKEGSPYTGFLYAGLMIKA
                     DGSTKTLEFNCRMGDPETQPIMMRLKNDLFTLIEHAVEGALDAVEAEWDRRVALCAVM
                     AAAGYPDHPRTGDLIKGLPRNGDDFHVFHAGTSVRDGKVVTNGGRVLCVTALGDTVRV
                     AQRRAYAIAEGIHFDGSQMRRDIGFRAIGAKKSTL"
     gene            14861..15757
                     /gene="hemF"
                     /locus_tag="VN496_07545"
     CDS             14861..15757
                     /gene="hemF"
                     /locus_tag="VN496_07545"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HXF16842.1"
                     /translation="MATERVKTYLTGLQAGIVGALSTLDGNAFRSDTWTRPAGGGGIS
                     CVIEDGDLFERGGVNFSHVSGRALPDSATAARPDLSGRSFQAMGVSLVLHPRNPYVPT
                     VHMNVRFFIAEKAGAAAVSWFGGGMDLTPYYGFEQDAVHFHRVCREALLPFGQHTHAR
                     FKKWCDEYFYLHHRREPRGVGGIFFDDLSEPDFDTCFALTRSVGDHLLEAYVPIVQRR
                     KDLPFGPSERDFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPPSVKWRYDW
                     RPEPGTPEAKLYTDFLTGRDWV"
     gene            15766..16044
                     /locus_tag="VN496_07550"
     CDS             15766..16044
                     /locus_tag="VN496_07550"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXF16843.1"
                     /translation="MNSHRIIVTVIGKDRVGIIASVSAELAQADANILDISQSVMDEF
                     FVMIMMVDIEHANIPFGELKARLNAKGESMGLKIEAQHEDVFKFMHRI"
     gene            16054..17424
                     /locus_tag="VN496_07555"
     CDS             16054..17424
                     /locus_tag="VN496_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HXF16844.1"
                     /translation="MSTSRYSPQEIIETIRMVQMENLDIRTITMGISLRDCSHPDLAV
                     AATKAYDKICRLAEDLVSAGEEIAAQYGIPIINKRISVTPIALIAESSDADSYVLMAD
                     YLDRAAKQVGVNFIGGFSALVQKGMTRGDSALIASIPEALATTDRVCASVNVGTTKAG
                     INMDAVALMGRTIIETARLTARRDGVGCAKLVVFCNAVDDNPFMAGAFHGIGEPECVI
                     NVGVSGPGAVLRAIEQEGCADFGALAATIKRTAFKITRMGELVGRAASDKLRVPFGIV
                     DLSLAPTPALGDSVARILEAMGLERVGTHGSTAALALLNDAVKKGGAMASSYVGGLSG
                     AFIPVSEDIGMIEAASMGALTFDKLEAMTCVCSVGLDMIAVPGDTPPETIAAIIADEA
                     AIGMINRKTTAVRIIPAPGKREGDMVEFGGLLGRAPVMAVHRESSRHFIERGGRIPAP
                     LQALNN"
     gene            complement(17431..18726)
                     /locus_tag="VN496_07560"
     CDS             complement(17431..18726)
                     /locus_tag="VN496_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXF16845.1"
                     /translation="MRQMQTATGRVTLKKKSSNLFRDRNRTEKARLDVQDFNQILEVD
                     PATGWIDAEGMASYEKVVLAGLACGVMPTVVPQLKTITLGGAVAGVGIEASSFRYGLV
                     HHGILEMEILAGDGNVYLCTPHNEHSDLFFGFPNSYGTLGYALRVRAKSIPVKKFVRL
                     EHSRFGDPGAYFRGLLALCDSAADFVDGVVFGPDEMYLSAGTFVDEAPFLSDYTFEHI
                     FYRSIREKPTDYLTVHDFLWRWDTDWFWCSKNFGVQIPFVRRIAGRERLNSRTYTKIM
                     RWNSRWKLTHRLDALSGVHRESVIQDVDIPITRAEEFLQFFTRDIGILPIWICPIRSP
                     DKRAFTLYPLDPSTLYVNFGFWDVVKSKKTQLPGHFNRLIERKVSQLGGMKSLYSDSY
                     YSRPEFDAIYGVQAYRALKSRYDPQSRLHDLYDKCVLRK"
     gene            complement(18785..>20012)
                     /locus_tag="VN496_07565"
     CDS             complement(18785..>20012)
                     /locus_tag="VN496_07565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXF16846.1"
                     /translation="REYMFTEVVRRRMLVLGDRVETCFRLQFSDGSQYQNRPGVPVFT
                     IIMRNARAGLRVLLFGHVGLLEAYFAGDVDVEGDFGAAFQVALASGFDHGSNALVTLR
                     NRWHELRFSNKSIAQARENARFHYAIGTEFYRYWLDTPLMMYTCGYWKEGTANVDEAQ
                     INKVDHVCRKIALSRGESVIDIGCGFGGFMFHAYERYGAIPRGLNTTPEQVTSVDKEI
                     GKRGLQGKIEVKLADFREVDRQYDKVVSIGVLEHAGRDQLAEVVKAHADFLKPGGLGM
                     LHFIGHVGVRDTEFYIRKHVFPGGWIPSLAQTISEMERCGLEIVDIENLRRHYALTLD
                     DWAERFERNWESIRALNPGRFNERFKRVWRTYLVSCAEMFRSSTSKTHLFQIVFSKGN
                     VTRKNYPMSRSFLYEH"
BASE COUNT         4356 a         5540 c         5720 g         4396 t
ORIGIN      
        1 gcttgcctgg ccccaaagcc tcgcgcgcca gagttacgtc tgcatcaagc tcgccgatat
       61 ggatgccatc agcgcggact gcctgggcaa gagcgatgct gtcattgaca atgaacagcg
      121 cattgtgtga acggcacgaa tctgcaatct cttcggcctg ggcgcggcga agtgagtcag
      181 gtatggactt ggcgcgatac tgtatcactt gagccccacc gcgtagagca gcgcgcactt
      241 tttggaataa tgctatggag tcagtcagct ccggcgttat cgcgtagagt ccgctaacgg
      301 gctttttcat cttccggctc ttcctcgcgg gcccagaaca gtcggtcagg aacgagttgt
      361 cccatgccgg ttctgtaagc gtgcttcagc gtctgccagg tatattcctg tgcgtcccga
      421 accgcttcgg gaacgcttag tccatttgcg atcgtcgccg cgatcgcgga cgcaagcgtg
      481 catcccgatc catggaatgt tcctgcaaga cgttgccagg aatccgctcg cacgacaccc
      541 tgctgttcgt agagcgtatt gaccacttgt ggagtatttt cgtgcgtgcc agtgatcaac
      601 acgtactcac agccgagacc aaggattctg ttggcacatt ctcccaagtc cggattatct
      661 tcgtcatctg catcatcttc ttgcattagg cgacgagctt ccagactgtt gggtgtaatt
      721 aacgtggtct gcggaattag aagttcgcgc atagccaaaa taagttcctg tgacgcaagt
      781 tcatcgcctc tgccggatgc caggaccggg tccagtacaa gaggaatatc ggggtaatct
      841 gacactacct ccgcgatcgc agcaacggta tctacactgc caagcaatcc gatcttgaac
      901 actgcaaccg gcacatcttc cagcacgcat cgcgcctgat cagccaccca ctctgcatct
      961 atggggagca cgtcctcgac tcccatggtg tcctggatgg ttaccgcggt gactaccgag
     1021 agaggatggc atcccatgct cgacagcgtg agaacatccg cttgcatgcc ggctccgccg
     1081 ctcggatctg taccggaaaa taccagaaca atgggaggcg aagcgggcac ggcgtttagg
     1141 cgaaggcaaa ttcgaactaa aatggaagtt taactgaaag ctttgatagc catgtccgca
     1201 agcggggaac aaacttacaa gacttttatg tgcctaattt gcggattcat ctatgacgaa
     1261 gcagaaggcc tcccagagga aggtcttcct gcaggaaccc gctgggaaga tgttccggtc
     1321 aattgggtct gtccggaatg cggtgcgcgc aaggaagact tcgaactggt cgaaatctga
     1381 atcaagggat gcatatgcga atactccata ccatgttgcg ggtcggggac ttgaagcgat
     1441 ccgttgattt ctacaccaac gtgctcggca tgcaggttct acggcaaaag gactatccag
     1501 acgggcgttt cacgctggcg tttgtcggat atggaacaga agataagaac tccgttctcg
     1561 aactgacgca taactgggat acgccaagat acgatttggg caacggctat gggcacattg
     1621 cgctggaagt caacgatgcc gccaaggcat gtgacgacgt caggcagcgc ggtggcaaag
     1681 tgacgcgtga agcaggaccg atgaaacatg gaactaccgt cattgcgttt gtggaagatc
     1741 ctgacgggta caagatagag ttcatacaac ggaaagctta ggaaacgcgt gcattcagct
     1801 cgcgatccga tttgcgatat tcacgaaatc ccgcactgcc aacgactctc ccctggctgc
     1861 cggatcaatt tcgagtttgg cgaaatccga tgcgtccaat tttcccctta acgtattgcg
     1921 tagcgtcttg cggcgttgcc caaatgcccg catgacgaca tcggcgaaca ccttttcgtc
     1981 gcgagcgaca tacggcaaaa ccgtgtgtgg gatcatgcgc accaccgagg accagacttt
     2041 tggagccggg cgaaaggcgg ttggtgacac atcgaacaaa gcatcaattt gccagcgata
     2101 ttgcagcatc accgacaggc gaccataggc tgaagaaccg gcttgcgcac tcatccgctg
     2161 aacgacttca cgctgcagca tgaaatgtgc gtcgcgcacg caggacgcgt ggtccgttaa
     2221 atgaaaaagt agtggcgtag aaatgttgta gggcaggttg cctaccacgc gcaagtcgcg
     2281 gccgaattgt gccaaatcga tttctagcgc gtcgacatca tgcacagtca accgattgac
     2341 gggatagcgg tcccgcagtt gctgcacgag gtcgcgatcg atttcaatga catcgaggtg
     2401 cccaacgcgt tcgagcaacg gcacagtcaa cgcaccgagg ccgggaccga tctcaatgag
     2461 ccgctgcgcg gagttcggac tgatcgcagc caccatctgg cccactaccg ctcgatcttc
     2521 caggaaatgc tgcccgaaac gcttacgcgg cctgtgcatg ctcggaattt tcccagaccg
     2581 gtagtgaacg gtaacgcaat tgatccctgc tgcgcgcgga agcgcgagct actgctcagt
     2641 gattttccag gcgcaattcg acgtaagcgc gatcccgttg ctggcgtacc cattcctggt
     2701 aagcctcatc ggccttgcgc gtacggatcg attgccttgc cagcacgcgc tgtcgctcct
     2761 ttgacatgtc ttcgttgcgc cgttcaatta cctggatcag gtgccaaccg aaaggggtct
     2821 gcaccgggtc actgacctgc cccggctgca gagcattcat ggcgcgctcg aactcgggga
     2881 ctgcgtcgcc cggcgatagc catcccagat ctccgccgcg agaggcggta gcgtcctccg
     2941 attgcaaacg cgccagttct gcgaaactcg tgtcgttttc gattcgctcc ttcagattgt
     3001 tcaggcgctg tttcgcttct gcctcggaca ccacttcgtt aagccggatc agaatgtgtc
     3061 tcgcatgagt ctgctggata attactggcg attcgttgcc acgcttgtct acaagcttga
     3121 ggatatgaaa tccattggcg ctgcgcagta ccggactgac atctccaacg ttcatactct
     3181 tcactgattc gacgaaaatc tgcggcaagc gcgacgcggt tcgccatccc aaagggcctc
     3241 cgtcgagtgc gttctgcgca tctgacactc ccgccgagac ctgccggaaa tcggccccac
     3301 ccttgatctg atcgagcgct ttctcggcta taacgcgctt cgatttgatt tgctccgggc
     3361 tggcctgctc gggaaccatg atcaaaatat gcgcaaggct atactcctcg tccttgccgc
     3421 tttggctttg ttcgtttttc aggtaattgt caatttcggc gtctgtgatc aggatcttgc
     3481 tatctacctc ccgctccttc agccgcgcaa tcagaatctc gttacgcaat tcctcccgga
     3541 atcggttgaa gtcgataccc tcggactcca tcgtctttcg aaattgctcc ggcgacacct
     3601 tgttctcctg cgcgatgcgc tcgatagcac gttgtaactg ggtatcgtcc acgcgcagcc
     3661 cggtttcctt ggcactttgc gccaacactt ttgaggtcac cagccgatcg agcaattggc
     3721 gctccaggac ttcgcgtgcc ggcagcggcg taccctgccg ctttagctgc atcagcgcga
     3781 cctttacctg ctcgtccagg tcgagtcggg tgacgacctc gctgttaacc acagccacga
     3841 cgcggtccaa agcaatcgcg tcatatttct tacgctcctc ctgcgctgcg ccaaccggaa
     3901 gagcgatgca tgcaatcaat actgcggcgt aaaggcttct cttcatcgta tgcaacgggc
     3961 actgtagcta aattcaaacc atcgaccaac agcggcagag cgaggtccac aagtcaaaac
     4021 gctgggaatg gattggattc cgaagtcctc tgatctgtgg ttttctgata gccggtgatg
     4081 ttttgccgca atacctccag gggactggaa ccgatctggg agacacccct gagctccagc
     4141 tgaaagaaca ttgcgttaac gtattcctga gtagcgctaa caaatcgatg catgacaaca
     4201 cgtgcggccc agcatccggc attgtactcg agccccaaca atccctcgag caggttcctg
     4261 tcctgcaagc tgtagttata acgcaccaac cccgtccagc gcccgccgat cggccattct
     4321 gtggaaaagt cagcctgctc cagcgagcct cgggtgtagc ggtaacctag gtttaccatt
     4381 ttgccgaaat cgggccggta acgcagtacc gcgtcggtgc gctgggtctg gctcgcaacg
     4441 gtgctgtact gcgtgttgac gtcggcgaac cagtattttg tcatttggcc ggttactcct
     4501 gccagcacat cggatcgcga tgagttgcgc acttctggcg tcagggagac ttcctgcggt
     4561 ttgaaataat agcgttggcc cagcatgaaa cgcatctgtt catcgcccga acccggatcg
     4621 atcaggcggc tggtgaccgc agccgttagc tgatttgcat cgttgatgcg atcaccgccg
     4681 gaaaactggt tttcggtgaa tatttgcgtg aaattaaggt cggcctgcgc ggtatcgaat
     4741 atcggcagtt gatcctgtgg acgaaacgga atgtagacat agtacagccg gggctccaga
     4801 gtttgtgaaa acgcccgtcc ccataccgtg gtatcgcgtt cgaaagtcag tccgctgtcc
     4861 aggctgtaga tcggcaaatt tctggtggca tcgggggtag ttgcatcctg aaaggaataa
     4921 ctcgtcgtgc tgaagccggc cttgggcgtt atataaaaaa atggcatctg gattggcgcg
     4981 ctgacagatg gatacaaaat ttcccgagtg gcattgagca aggttggatg actgaagtga
     5041 acaaattccc cttgcaatcc gaggtcgagg cgaatatcag tcgatcggtt catgtttaac
     5101 gttatctgcg gcacccttgc gtagggcggg actacgggag caagtgggtc ctgcaaagtc
     5161 tggtaacgct gaacacgcgt attaacgttc caccacccgc catcgtagaa aagatcgcct
     5221 tcccgattca ggttagtttg tgacgtggca gcaatcttgt cgctcaaatc ggtgaaataa
     5281 gtgtcatccg aaacgccctg tacatctact gcgcccgaaa agccattacc gaaattttgc
     5341 tggtgctgcc ataaatatgc gtaacggctc tcgcctttct gacgatcgtc aggcaagtac
     5401 tccgcgcgtg cttcaccttt aagagtcggc tccaaatagc ggaaatcgcc gttcatcagc
     5461 acgccgcgct tggaaagaat acgcggcgaa atggttgcat cctgattcgg tgcgatattc
     5521 cagtagaacg gttgggtaaa ctcgaagcca ctgctgcccg tgctgccgat ggtcggaggc
     5581 agcagtccgg tctttcggcg accgctcaac gaaaaatcca gataggggga atacaggatc
     5641 gggacgcctt tgaagacaac ggtcgcattg cgtgcaacgc caatatcgct gattctatcc
     5701 aactcgagcc ggttgatgcg catcagccaa tcgtcatctc ccacgtcgca agtggtatac
     5761 gtcgccttgt cgatgcgggt cagattggga ttttcggcga ccagccggtc ggcattcccg
     5821 cgggcgccgt accgcgtcag ttggtacgtc ggcttgtcta tattgccgct ttcatagttc
     5881 agatcgtaga acagcagttc accggtgacg atgtcgcctt ccttttcgaa gcgcacgtgc
     5941 cccgtagcag tcacctgctc ttctggaata ttcagatgca cctgatccga aaataccgcg
     6001 gtaccgcgct tgcgaacgcg cacgtttccg ctcgcatcaa gctcttgttc ctggttgcct
     6061 tcgatcttgt ccgcctcgat gaacagcgtg gacggttcat cgcgtccaag ggagtaggga
     6121 atgagatcgg gctgcatgcg cagctttaat ccggggtttt cagcgtgcac gtccaaagcg
     6181 acatacagga tcaacgacag tacagcagat gtgatgcgtg aatggtgcat gcgacctgtt
     6241 gatccagacg aggtgctaaa atctcataaa ttatccttca aggcaacgca gcatcttgga
     6301 tcgttacgcg cttgttcagg actggttgag ccggcagttt gagggacaga gcttttctct
     6361 tgatccggct tcgtcggatg caagcttcag gcgctacttc cgagttactt tgcgcgacgc
     6421 aacgttcatc gtcatggacg ccccgcccga tcgcgaagac tgccgtccct tcctacgcgt
     6481 agcaaggctc atgtcctcag ctggcctgca tgtcccggcc attcttgcgc aggatctaga
     6541 gctgggcttt ctattattgt ctgacctggg ttcgacgacg tatctcgctg cgctcagcaa
     6601 tggcaatgca gatgagttgt accgcgatgc aacggatgcc ttgattctgt ggcagcgcgc
     6661 aagcgtcgaa ggcgtgctgc cgctctatga cgaagccttg ttgcagcgag aactggatct
     6721 atttccggag tggtatctgc gccgtcacct tggtctggaa ctcgatgcgc agcaaaagcg
     6781 gtctcttgcc ggcgtcttca agctcataat cgccaacaat ataagccaac cgaggagcta
     6841 cgttcatcgg gattacatgc cacgcaacct gatgttgagc acgccgaatc cgggcgtact
     6901 ggattttcag gatgcggttt acgggccaat cacctatgac gtagcttcac tgttcaagga
     6961 cgcgttcatt agctgggacg aaggacgcgt catcgattgg accatccgtt actgggagcg
     7021 cgcgcgatcc gcaggcttac cggtcagaag cgattttggc gagttctacc gggattgcga
     7081 atggatggga ctgcagcgtc acctgaaagt actcggcatt ttcgcccgca tctgccaccg
     7141 cgacggcaag ccagcttatg tcaaggatac ccctcgcttc gtcgcttatg tgcgggcagt
     7201 atgcgagcgc tatgcgtcgt tgtcgccttt gcttcgcctg cttgatcaaa tagccgagcg
     7261 cgagccggta tctggccaga ctcgctgaac atgtgacagc gagcgcctct ttcaccgcga
     7321 tgattcttgc cgccggacgc ggggagcgca tgcgaccgct taccgatcgc atacccaagt
     7381 gtttgctgaa ggtcgacggc aaaccactca tcttccggca cctggaaaag ctagcagagg
     7441 cgggttttgg cacggtggtc atcaaccacg cgcatctcgg tgcacaaatc gaggaggccg
     7501 taggagatgg ccgcagatgg aatatccgga tccgcttttc cccggaaccc caggcgctgg
     7561 agactgcggg cggcattctt aacgccctgc cattgctgca tgaaccggtc tttgcggtcg
     7621 taaatgcgga cgtttacagc acttatgatt atggcaacct cgtcttggga atcgggagaa
     7681 tgcaagcgac cacgcttgct cacctggtcc tggtgcccaa tccaccgcat catcgtgatg
     7741 gcgatttttc gctggaaggc aaccaggtgc ggatgggcac gaaagaacgg ctgacgttca
     7801 gcggcatagg tgtctatcgc gcgaccctgt ttgatcaact cgtgcccggg agcaggcatc
     7861 cattggctcc gctactcgtt aagcaaatgg ctttgggcag aattagcggc gaatacttcc
     7921 ggggcgagtg ggacgatatt ggcacgcctc agcgtcttgc ccaactggag gcgagactgg
     7981 cgcgcacggc agagcactaa agctcaggcc cataccaaac cgtataatcc agagttttct
     8041 ccgtccgcat tttttcccca tgattgatcc agctctgtat gtccgccgcc gggcccaact
     8101 ggccgcgctg atgcaggaag gcgtagcagt agtccgcact gctccggagc ggccgcgtaa
     8161 tcgtgattca tcttttccat ttcgctttga cagctatttc cattacctca ccggttttcc
     8221 ggaacccgaa gcggttttgg tgctattggc agaaagtccc gtgaaaagca ttttgttctg
     8281 ccgtgacaag gatacggagc gggaaatatg ggacgggttt cgttttggcc ccgaccaggc
     8341 gcgcgaaacg ttcggatttg atgaagcgca tcctattacc aagcttgatg aattattgcc
     8401 aaaactgctc gcaaatcgaa gcaccgtctt ctgcgatgtc ggtgcggatt cgagttggga
     8461 tgtgcagatc atcggatgga tcaataaggt gcgcgctcaa gtcagggccg gggtcagcgc
     8521 accaagcgag attcgcgaca tccgccggtt cctggacgat atgcggctca tcaaggatga
     8581 acacgagatt ggaatcatga ggcgtgcagc caacatctcc tcggaagcgc accgccgcgc
     8641 catgcgcgcc acgcacgccg gtttgcatga atacgagctg gaggctgaat taatccacga
     8701 atttcggcgc cagggcgcgc aagctccggc atatacaccg atagtggccg gcggaggaaa
     8761 ttcctgtgtc cttcattatg tgcagaacga tggcttgctc aaagacggag atgttgtgct
     8821 gatcgatgca ggttgtgaac ttgatggcta cgcttccgat atcacgcgaa cgtttccggt
     8881 gagcggtata ttctcaggac cacaacgcga tatctaccaa ctggtactgg cggcgcaggc
     8941 agccgcgata gcccaagtga agcctggaaa tgattgggaa gcgccgcacg atgcggccgt
     9001 aagagtgctg gcacggggca tgattgacct ggggttatgc aaaggcacga cagacagtgt
     9061 gatcgaatcg ggcgattacc ggcgcttcta tatgcatcgt acgggacact ggctcggtat
     9121 ggatgtacac gatgccggag actacaagcg caatggcgcc tggcttaaac tccagccggg
     9181 catggtgctg actgttgagc caggctgcta cgtgcgaccg ggggacaatg ttccggagca
     9241 gttctggaac atcggcgtgc gtatagagga tgatgcactg gtcactgaat tcggcagcga
     9301 aattatttct tcggcggcgc caaagactat cgaacagata gagtcgttga tgaaccactg
     9361 aacatgcggc atgactatgt gcaatcgagt gcggcacagg ccatccggtt atgttgtcca
     9421 aaactgacta cgacatcgta attgtcggcg gcggtccggt tggtgcaggc gtcaccattg
     9481 cactgcaggc cagtgggttc tctgtgctgt tgttggaatc gcggatcgat aacagcaaca
     9541 gtgatgacca gcgcaccttg gcgctgtcct ttggcagtcg cctgattctc gagcgcctcg
     9601 gcctgtggtc gcaaatcggg ccggctacgc caattcaatc cattcacgtg tcccagcgag
     9661 gcggattcgg ccgcacattg ctattggcgc aggagttgga attgccggca ttgggttacg
     9721 tcattcgcta ctccttgctg caacaaaccg ttaatcgtgc gctgaaatca ctggcagcga
     9781 acttgatgac gggtgcacaa gctgtcagca tggactcgac gagccaagcg attgagatcg
     9841 gtattcagac gcagggaagc cggcaagcca tatcagcccg attattggtc attgctgatg
     9901 gtggggccgg tctgggattg cagtctggcg caacgacgaa agttcaggag tacgggcaag
     9961 acgccgtcgt gggcctggtc ggaacatcac aaccgcacgc gaaccgagcc tttgaacgat
    10021 tcacgccaga tggcccgatc gccttgctgc ctttccagaa tggttatgcg ctagtgtgga
    10081 ctgcggctcc ggaaacggcg gatgccttgc tagctttgcc agcatcaaca ttcctcgata
    10141 aattgcaatt acatttcggc gatcgtgcgg gccgtttcag ttcgattgcg gcacgtgccc
    10201 ggtttccgct gaccttgcgt tacgccgccg accccgtgat gccgcgtgca gtcatgctgg
    10261 gcaacgcagc gcagtctctg caccctattg ctggccaggg gttcaatctg ggtttgcgcg
    10321 acgcttggga attaagtgaa gtgttgctgc ttcacggaaa ggctgatccc ggatccagcg
    10381 cctgcttgtc gcgttatcgc gccgggcggc ggcccgaccg cttgcgcagc atcgggctaa
    10441 cccattcgct ggttaagatt ttctccaatg atgttttgcc gctgagggcc gcgcgcggcg
    10501 cggcgctgtc gctgctcgac ctggtaccgt ccggcaaacg cgcgttcatg cagcgcatga
    10561 tatttggcgc tttgcgttaa acgacaaagc ccggcttcca tcgcgcattc tttgcgctag
    10621 aatggcggtc caaatttttt cccgtggcgc cgaaatgcag atcggtccgc atgccctcag
    10681 gaacaatctg atcgtcgcgc ccatggccgg agtaaccgat cggccattcc gccagttgtg
    10741 caaggcaatg ggtgccggga tggcggtatc ggaaatgatc tcatccaatg cgcaacttcg
    10801 cgataccgtg aaatccatgc gccgctcaaa tcatgagggt gaagcggatc cgatttcagt
    10861 gcagattgcc ggcgcagatc ctggcgccat ggcggaagct gcccgctata acacggaccg
    10921 cggcgcgcag atcatcgaca tcaacatggg ttgccccgcc aagaaagtct gcaacgtcgc
    10981 ggccggatcc gccttgctgc aaaacgagcc gctggtcggg aaaatccttg atgccgtcgt
    11041 ggcggccgtt aacattccgg tcaccctgaa gatccgcacc ggatggagtc cgcataatcg
    11101 aaatgccgtt cgcgttgcgc aaatcgcgca gtcggccggc gtgaaagcat tatcggttca
    11161 cggccgcaca cgcgcttgtg ctttccttgg gcacgcggaa tacgaaacga ttgccgaggt
    11221 aaagtcgcgc gttgccattc cagttattgc taatggcgac atcgattcgc ctgaaaaggc
    11281 ccgtgaagta ctgcggctca ccggtgccga tggtctgatg attggccgtg cagcgcaggg
    11341 gcggccttgg attttccgcg agattcagca ctacctgatc aacgacaaac atctgccgcc
    11401 gccagacatc gcggaaatta aagaactact agtgaaccat ttgcatgccg tctacgaact
    11461 gtacggcgaa caaagtggcg tacgtgtcgc gcgcaagcac atcggctggt atgtcaaggg
    11521 gcttgctggt gcatcgcttt tccgacaaag catgaacaaa atggaaatga cgcatcagca
    11581 actcaatgcg gtggaagcct tcctcgacgg gctggcaaca cgtggagacc gcctgactta
    11641 ttcggaggag gaactggcgg catgatcaat gacaatgaaa tagcacgctg cgtgcgccgc
    11701 gcgatggatg gttacttcaa ggatctggaa ggcgaacggc cttgcgcagt ttacgatatg
    11761 gtcatgaact cagttgaaaa gccgctgctt gaaatcgtgc tccaccgtgt atcgggaaac
    11821 cagacgcagg ctgcgcagtt gcttgggatg aaccgtaaca ctttgcgtaa gaagatgaaa
    11881 tattacggca tcaagtagcc cttgatgccg ctccaccaag ccgcccgtgc attcgcgacg
    11941 ggcggttgat ttttggattt cgtaaatgaa caggcgaaaa gcattagtca gtgtgtcaga
    12001 caagagcgga gcggcggatt ttgcacgctt cctgcacgag attggcatgg agatcttatc
    12061 cactggtgga accgccaaac tgctgcgaga ggcgaatgtg ccggttagcg aaattggcga
    12121 ttacaccggg tttccggaga tgctggacgg ccgggtcaag actttgcatc cgaaagtgca
    12181 cgccggcatt ctggcgcgcc gcgacctgcc ggaacatcta aatgccacag cccgcgccgc
    12241 gatcccgctt atcgatctgg tagtcgtaaa cctctatccg tttgcgcaga cggtctccag
    12301 ggcaggatgt acccttgagg aggccgttga aaatatcgac atcggtggtc cgactatggt
    12361 gcgtgccgca gctaaaaact ggcagcatgt ggccattgtt acggatccgg cggactatcc
    12421 ggcgttaatc gcggaaatga aatcggccag tggcgcaatc agcggcgaga cgcgattctg
    12481 gctggcaaag aaggcttttt cgcacacggc ggcatacgac gcggcgatca gtaactattt
    12541 gaccgccaca gatgccagcg gtgcccgtgt cgagtttccc gatcagatta atctgaacta
    12601 cgccaaggtt caggatttgc ggtatggaga aaacccgcat cagcaggcag cgttttaccg
    12661 cgagctaaaa agggttccag gtgcgcttag caactaccgc cagcttcagg gcaaggaatt
    12721 gtctttcaac aatattggcg acgcggacgc ggcttgggaa tgcgtcaaaa cattcgcgga
    12781 gccagcctgc gtgatcgtca agcacgcgaa tccgtgcggc gtggcggtgt cggtcaattg
    12841 ccttgaagcg tatagccgcg cgttcaaaac agatcccaca tccgcattcg gcggcattat
    12901 tgcgttcaat cggcagatcg atgtggagac ggctgaagca ctgacacagc aatttatgga
    12961 agttctgatt gcgccaggca tcgcggatga tgcggcccgg gtactagcca ggaaaaccaa
    13021 cgtacgggtc ctcgagatag cgagtgaagc cggtgcgaac cgattcgact ttaagcgcgt
    13081 tggaggcggc atgctcgtgc aaacgcccga tacgatgaac gtacaattga acgatttgcg
    13141 tatcgtcaca aaaaagcaac cgtcttccgg gcagctggca gacttgatgt ttgcctggag
    13201 agttgcgaag ttcgtcaagt ccaacgcgat cgtattctgc ggggatggga tgacccttgg
    13261 ggtcggcgcc ggtcaaatga gccgggtcga tagcgcgcgc atcgcgacaa tcaaagccga
    13321 aaacgccggc ctgtctctag caggttccgt agtagcttct gacgctttct ttccgtttcg
    13381 ggacggggtg gatgtagtcg caaaagctgg agccattgca ataatccagc cgggcggcag
    13441 catgcgcgat gaagaagtca tcgcggccgc ggatgagcat ggaatcatca tggctctgac
    13501 cggcgtgcgt catttccgcc attgaatgat attgtcttgt cttacctccg ttcgtcgtcg
    13561 gtaacgtctc tatgaacctt ctggttatcg gcagtggcgg ccgcgagcat gcgctggctt
    13621 ggcgcttggc acaatcgccc cgcatcgggc gcatttatgt tgcgccgggt aatgctggca
    13681 ccgctcagga agatggcctg ttcaatgtcc cccttaccgc gattccagat ctgcttgatt
    13741 tcgcgcgcaa ggaagccgtc gcattgacgg tggtcgggcc ggaagcaccg ctagcggcgg
    13801 gaatcgtcga cacgttccgg tctgcaggac tgcgcatctt cggacctacg cgcgcggccg
    13861 cgcaactaga agcatcgaag gacttcgcta aagcatttat ggtccggcat ggtatcccga
    13921 ctgctgcata tgccagtttt acggacgcaa aagcggcaca cgactacgtg gctcaacgag
    13981 gtgctccgat cgtcataaag gccgatggac tggccgcagg caaaggcgtc gtcgtggcca
    14041 ccacgccaga ggaggcacat gccgcgattg atttgatgct ggtctccaac aaaatgggag
    14101 tggctggagc gcgcgtcgtc attgaagaat ttctcgaagg cgaagaagcc agtttcatcg
    14161 tgatggtgga tggcaagcat gtgctgccgc tcgcctccag ccaggaccac aagcgtctgc
    14221 tcgatgccga caaaggcccc aatacgggcg gcatgggcgc ttattcgcct gcgcctgtcc
    14281 ttacgccagc cattcacgcg cgcgtcatgc gcgaagtcat acaaccggta attcaaggaa
    14341 tggaaaagga aggcagccct tataccggct tcctgtacgc agggctcatg atcaaagcgg
    14401 acggcagtac aaaaactctg gagttcaatt gccgcatggg agatccggaa acccagccga
    14461 tcatgatgcg actgaagaac gatttgttca cgctgatcga acatgccgtc gagggagcac
    14521 tggacgcagt cgaagcagag tgggatcggc gggtcgcact gtgtgccgtc atggccgctg
    14581 ccggttatcc ggatcacccc cgcacaggcg atctgattaa ggggctgccg cgcaatggtg
    14641 acgatttcca tgtcttccat gcaggcacct ccgtgcgaga cggcaaggtg gtgactaacg
    14701 gcggacgcgt gttgtgtgta actgctctag gcgataccgt gcgggttgca caacgcaggg
    14761 catacgcaat tgccgaaggg atccacttcg atggtagtca aatgcgccgc gatatcggct
    14821 ttcgcgctat cggcgcaaag aaaagcacat tgtaatttcg atggcaacgg aacgggtcaa
    14881 aacatatctg accggtctgc aggctggcat cgtcggtgca ctttcgactc tcgatggcaa
    14941 tgcgttccga agcgatacct ggacgcgtcc ggctggcggc ggcggtatca gctgcgtgat
    15001 cgaggacggc gacctgtttg aacgcggcgg cgtcaatttt tcacacgtga gcggccgtgc
    15061 cttgccggac tcggccaccg cggcgcggcc tgacctttcc ggccgatctt tccaggcgat
    15121 gggcgtttcc ctggtcttgc atccgcgcaa tccctacgtg ccgactgtgc acatgaatgt
    15181 gcgttttttc atcgcggaaa aagcgggggc cgcggcggta tcctggtttg gaggcggcat
    15241 ggacttgacg ccttactacg gattcgagca agacgcggtt cactttcatc gcgtttgtcg
    15301 agaggcacta ctcccattcg gacagcatac gcatgccaga ttcaaaaaat ggtgtgacga
    15361 gtatttctat ctccatcatc gccgcgaacc gcgcggggtc ggtggtatct ttttcgatga
    15421 cctgtccgag ccggatttcg acacctgctt cgcactcacg cgcagcgtag gtgatcatct
    15481 tctggaagcg tacgtgccaa tcgtgcaacg gcgcaaggat ctgccgttcg gcccttcgga
    15541 gcgcgatttc caggcctatc gccgcggtcg ttacgttgag ttcaatctgg tctacgaccg
    15601 aggcacactg ttcggactgc agtccggtgg ccgtacagag tccattctta tgtctcttcc
    15661 gccgtcggtg aaatggcgct atgactggag gcctgagccc ggcacgcccg aggcgaagct
    15721 ctataccgac ttcctcactg gtcgcgactg ggtttgagtc caatcatgaa ttcgcaccgt
    15781 atcatcgtga ccgtcatagg caaggatcgc gtcggcatca tcgccagcgt ttcagcggaa
    15841 ttggcacagg ctgatgccaa tatcctggac atatcgcaat ccgtcatgga tgaattcttc
    15901 gtgatgatca tgatggtcga catcgagcat gccaatattc cgttcggcga actgaaggca
    15961 aggctcaacg ccaagggcga gagcatgggg ctcaagatcg aagctcagca cgaggacgtg
    16021 ttcaagttca tgcacaggat ctgagactcg acgatgagca cgagccgata ttccccgcag
    16081 gagatcatcg agacgatccg tatggtgcag atggagaatc tcgacatccg caccatcacc
    16141 atgggaatca gcctgcggga ttgctcacac ccggatctgg cggttgccgc aacaaaagcc
    16201 tatgacaaga tctgccggct tgcagaagac ctggtttccg ccggcgaaga gattgccgcg
    16261 caatacggca taccgattat aaacaagcgc atctcggtga cgccgattgc attgatcgcg
    16321 gaaagctctg acgcggactc ttacgtgttg atggccgact atcttgatcg tgcggcgaag
    16381 caggtcgggg tcaatttcat cggagggttt tccgcgctgg tacagaaagg catgacgcgt
    16441 ggagacagtg cgctaatcgc gtctattcct gaagcactgg ccacgacgga ccgcgtttgc
    16501 gcctcggtca acgtcggcac gacgaaggcc ggcataaaca tggacgcggt cgcgctgatg
    16561 ggacgcacga ttattgaaac cgctcgcctg actgcgcggc gggatggcgt gggatgcgcc
    16621 aaactggtcg tcttctgcaa cgccgtggat gacaatcctt tcatggccgg cgcattccat
    16681 ggcattggag agccggaatg cgtgatcaat gtaggcgtgt ccgggccggg tgcggttctg
    16741 cgtgctatcg aacaggaggg ttgtgccgac ttcggtgcgt tggccgcaac gatcaagcgc
    16801 accgcattca agattacacg catgggcgaa ctggttggac gcgcggcttc agataaactt
    16861 cgcgtaccgt tcgggattgt cgacttgtcg cttgcgccca ctccggcgct gggcgactcc
    16921 gtagcgcgaa ttctcgaagc catgggactc gagcgcgtcg gcacacatgg gtcgacggcg
    16981 gcattggcgc tgctgaacga cgcagtcaaa aagggcggcg ccatggcatc cagctacgtg
    17041 ggtggtctgt cgggcgcatt catcccggtt tccgaggaca tcggcatgat cgaggcagca
    17101 agcatgggag cattgacgtt cgacaaactg gaagccatga cttgcgtttg ttccgtcgga
    17161 ctagatatga tcgccgtccc gggcgacacg ccaccggaaa cgatcgcggc aatcattgca
    17221 gacgaagcgg ccattggcat gatcaaccgc aagaccactg ccgtgcgcat tatccctgca
    17281 ccgggcaagc gagaaggaga catggtcgag ttcggcggat tattgggccg cgcgccggtc
    17341 atggccgtac atcgtgaatc cagccggcac ttcattgaac gcggcggacg catcccggcg
    17401 ccgttgcagg cactcaataa ctaggacaag tcatttgcgc agcacgcact tgtcatacaa
    17461 atcgtgcagc cgactctgcg gatcgtagcg cgattttagc gcgcggtacg cttgtacgcc
    17521 gtagatggca tcgaactctg gtcttgaata atagctgtcg gaatacaacg atttcatgcc
    17581 cccaagttgc gacaccttgc gttcgatcag ccggttgaaa tggccgggaa gctgagtctt
    17641 ctttgatttg acgacatccc agaatccgaa attcacatat agcgtcgaag ggtccagcgg
    17701 ataaagcgtg aatgcccgtt tgtcgggact gcggatcgga cagatccaga tcggcagaat
    17761 gccaatgtcg cgcgtaaaga actgcaggaa ctcttcggcg cgcgtgatcg ggatgtcgac
    17821 atcctggata accgattccc gatgcacgcc cgagagtgca tccagccgat gggtcagctt
    17881 ccagcgactg ttccagcgca tgattttcgt ataggtacgc gagttaagcc gttcgcggcc
    17941 agcgatccgg cgaacgaaag gaatttgcac accgaaattt ttggagcacc agaaccagtc
    18001 ggtatcccag cgccacaaaa aatcgtgcac cgtcaaatag tccgtcggct tctcgcgaat
    18061 ggagcggtag aagatgtgct caaaagtgta gtcgctcaag aaaggggctt cgtcgacgaa
    18121 agttccggca ctcagataca tttcgtccgg gccgaaaacc acgccgtcga cgaagtcagc
    18181 cgcagaatca cacagcgcca gcagtccgcg gaaatacgct ccggggtcgc cgaatcttga
    18241 gtgttccagg cggacgaatt tcttcaccgg tatggattta gcgcgcactc gcagcgcata
    18301 gccgagcgtc ccataagaat tcgggaagcc gaagaacaga tcactatgct cgttatgcgg
    18361 ggtacagagg taaacgttac cgtcgcccgc gagaatttcc atttccagta tgccgtgatg
    18421 caccagcccg tatcgaaatg agctggcttc gataccgacg cctgccaccg caccaccaag
    18481 cgtaatggtc ttgagttgcg gcaccacggt gggcattacg ccgcacgcca gtccggccaa
    18541 aaccactttc tcatatgaag ccatgccttc ggcatcgatc cacccggtgg cgggatcaac
    18601 ctccaggatc tgattgaaat cctgtacgtc aaggcgggct ttttctgtgc gattgcgatc
    18661 gcgaaataaa ttcgagcttt ttttcttcag cgtgacacgg ccggtggccg tttgcatctg
    18721 ccgcaccact ctttcccggc gcgcggcatg ggtgtcccgt gagtgagcct tcgcgaacac
    18781 tgtgttaatg ctcgtagaga aagctgcggc tcatcgggta attcttgcga gtgacattcc
    18841 ctttgctgaa cacgatctgg aacaaatgag tcttactggt tgacgaacga aacatttcgg
    18901 cgcaggaaac cagatacgtg cgccagaccc gcttgaatct ttcgttgaat cgaccggggt
    18961 tcagcgcgcg aatgctttcc caattccgtt caaagcgctc ggcccagtcg tccagcgtca
    19021 acgcgtaatg ccgtcgcaga ttttcgatgt ccacgatctc cagcccgcag cgttccattt
    19081 ccgagatagt ctgggcaagg ctgggaatcc accctccggg aaatacatgc ttgcggatgt
    19141 agaactcggt atcccgaacg ccaacatgac caatgaaatg cagcatgcca aggccgccgg
    19201 gtttcagaaa gtcagcatgg gcctttacaa cttccgcgag ttgatcgcgg ccggcatgtt
    19261 ccagcacgcc tatggaaacc actttgtcgt attgcctgtc gacttcgcga aagtccgcga
    19321 gcttgacttc aatcttgccc tgaaggccac gcttgccgat ttccttatcg accgaagtaa
    19381 cttgctcggg agtcgtattc agcccacggg gtatcgcgcc ataacgttca tatgcatgaa
    19441 acatgaagcc gccaaaaccg cagccgatat cgatgacgct ctcgcctctg gacagcgcaa
    19501 tcttgcggca gacatgatcc actttgttga tctgggcttc gtctacgttt gccgtccctt
    19561 ctttccagta accgcatgtg tacatcatta gcggagtatc gagccaataa cggtaaaact
    19621 ccgtgccgat ggcatagtga aaccgcgcgt tttccctggc ttgtgcaatg ctcttattgg
    19681 aaaatcgaag ttcgtgccag cgattgcgca aggtcacgag tgcgttggag ccatggtcga
    19741 atccactcgc gagcgcaacc tggaatgcgg cgccgaaatc cccctctacg tcaacatcgc
    19801 cggcgaagta ggcttccagc aagccgacgt gtccaaacag cagcacgcgc aggccggctc
    19861 tggcattgcg catgatgatg gtgaataccg gcacaccggg ccggttctgg tattggctac
    19921 cgtccgaaaa ttgcaggcgg aagcaggttt cgacgcgatc tcccaatacc aacatcctgc
    19981 gccgcacgac ttcggtgaac atgtactccc tc
//
LOCUS       DATMZQ010000231         9914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_177269, whole genome shotgun sequence.
ACCESSION   DATMZQ010000231 DATMZQ010000000
VERSION     DATMZQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177269"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VN496_07570"
     CDS             complement(<1..986)
                     /locus_tag="VN496_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF16847.1"
                     /translation="MSPRPLTEFLRAVSAFRRYANPERLPDWEQRFAFGLGDIEQVFE
                     AAVAEAPVLSAPKATVTEQFEAGRYVSSAPAGGLVSASAAQIAHQVRHHALSPVKLAE
                     LFLGRIDAHRHLNAFITVNPSLVMEEAQVLAHRLRRGEDPGPLSGVPVGVKDLMSVRG
                     YPLTAGTRAIEGKVQERDAPVVARLRAAGALIIGTTNLHELAFGISSANPHFGHVQNP
                     RYPGYIPGGSSGGSAAAVAAELAAVAVGSCTGGSIRLPAACCGIVGFKPTYDAVPREG
                     VYPLAWSLDHIGPITRTVEDAALAFEVMAGFPEHSMLPHTAITPPRIMQPRK"
     gene            1227..4265
                     /locus_tag="VN496_07575"
     CDS             1227..4265
                     /locus_tag="VN496_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF16848.1"
                     /translation="MTSPTLPSNERPQPRERFLLYLVPGLAVLLVITLFALAFFMLSK
                     SRENALRDGERTSAFLAQIIEERTGRLLQSVDQALGSTVYAWQRSPALRDPSGFALHA
                     LLAERVAQLPNARTLFVLDARGILLQDSDMRSAAHIDLSDRDYFSWHRAHTGNRLYVS
                     RPAEQSNGGWFIVATRRLEDTEGHFAGAVGVALEPAAFKTLLDRLDVGQAGTSALLHE
                     QGELIARAPEAPKWLGVPGKATPGQDSRTYRKVSEVDGVLRIYGVRPVAGTPLIVQVG
                     LSEDELLAGWRHQLNVAAAILAAFTLATVLLTWLLVQGLMRRNTLARSVGRNERMLRQ
                     VFDTLPVGVRVADRAGHVVLSNAASGRIWGDGEKDLHYFTDRKKELPQTADRGMALTL
                     TRGASSRNEVLDIECSDGSRKTVLHSTAPIVSAEGEIVGGVAVNEDITEQRALVDTLR
                     ESEARYQALFEHSIDAVLLMRSDGRILSANEQACRLLGYTESELRKLGREGLTEPDDP
                     RVEAMVEEHRREGGARGELKMRRKDGGWVPVEVSSTTFRDRSGKLCASLIIRDITDRK
                     RAEEHIEYLAYHDELTGIPNRAHFQRAFEQAITIHQRQGLKSALMVVDLDRFKNINDI
                     IGHEAGDQLLKQIAARLRTCLRDSDVLARLGGDEFVILMQDAASVEAVSAVANKILEA
                     TSRPLTIDEQEFVITASIGISTSPHDGTDLQTLLRNSDVAMYRAKESGKNGFQYFSPD
                     MDAHGRERLSIESSLGRALERQEFMLQFQPKMQVSSRNVAGMEALVRWQNPEKGLMQP
                     SAFIGIAEETGMIVPIGDWVLTEACRQGQALRDAGHAGLRVAVNLSVRQLYDDGLAMR
                     VSDALKRTGFPAENLELEITESMVMRDAEGAIKVLQALRDTGVRIAIDDFGTGYSSLA
                     YLKRFPIDCVKIDRSFIRDLPDDRDDASITRSIIAMAHNMNLEVVAEGVETVPQLEFL
                     HAYGCDEIQGFLFSKPLGAEAFEQFLDGQASPPPAFHLQA"
     gene            4338..5147
                     /locus_tag="VN496_07580"
     CDS             4338..5147
                     /locus_tag="VN496_07580"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXF16849.1"
                     /translation="MSATAAAQHVHVEPILLRKDVGGIATITLNRPKQYNSLSEELLT
                     ELQSMLDVVGKDTSVRVVVIAGSGPAFCAGHDLKQMRADPRKSYYDKLFAQCSDVMMK
                     LLHLPQPVIARVHGIATAAGCQLVAQCDLAVACDTARFAVSGINVGLFCSTPSVALGR
                     NVSRKQAMEMLLTGDFIDAATAKQRGLVNRVVPEAQLDTEVKALAEAIIAKSAVAVTT
                     GKKMFYKQMEMGMQAAYEYAAEVMACNMMAEDVGEGIDAFMQKRPPRWHDK"
     gene            complement(5159..7066)
                     /gene="htpG"
                     /locus_tag="VN496_07585"
     CDS             complement(5159..7066)
                     /gene="htpG"
                     /locus_tag="VN496_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HXF16850.1"
                     /translation="MGEAATRETLGFQTEVKQLLQLMIHSLYSNKEIFLRELISNGSD
                     ACDKLRFEALTDPGLFEDDPDLKIRVSLDRAARTITVSDNGIGMSRQEVIDNIGTIAK
                     SGTREFFGRLTGDQAKDASLIGQFGVGFYSCFIVADKVTLTTRRAGLGKEHGVRWESG
                     GEGDYTIEIVERSSRGTEVTLHLREGEDELLEGFRLQSIIRKYSDHISLPILMNKQDW
                     DKDKAEHVTRDELEKVNQASALWARPKSEITEQQYREFYKHVSHDFEEPLAWVHAKVE
                     GKSEYTQLLYIPARAPFDLWDREHRHGIKLYVRRVFIMDDAEQLLPAYLRFVRGVVDS
                     NDLPLNVSREILQESKDVETIRKGCVNKVLGLLEDLAQTQSEKYATCWKEFGRVLKEG
                     VGEDWSNKERIARLLRFASTHADSEEQSVSLSDYVARMKDGQAKIYYITADSFAAAKN
                     SPHLEIFRKKGIEVLLLSDRVDEWVGSNLTEFEGKQMASVAKGDLDLGKLADEDKKEQ
                     EKEAGENKELVENIGKSLGDRVKEVRVTLRLTSSPACLVADQHGMSGNLERMLKAAGQ
                     TVPGSKPVLEINPHHPLVQLLKYETDKGRFGDWSHILFDQALLAEGGQLDDPASFVKR
                     LNEMMLTLANK"
     gene            7205..8305
                     /locus_tag="VN496_07590"
     CDS             7205..8305
                     /locus_tag="VN496_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXF16851.1"
                     /translation="MSPRVAYLLFALAFFAGTVAATPPLDHIKLPAGFKIDVYAEVEN
                     ARSLTLGDNGTVFVGTRTDKVYALVDSNRDGKADKVVTVAQGLNAPNGVAFGDGALYV
                     AEINRILRFDNIEANLAKPPKPALVFDKLPRESHHGWKFIAFGPDGWLYVPVGAPCNI
                     CEANPDVYAAIHRMKPDGSQFQVFARGIRNTVGFDWDPRTQELWFNDHGRDMMGDDMP
                     SCELNHAPKAGMHFGFPYCHQGDTPDPEYGAKRNCSEFVPPALKQGGHVAPNGLRFYT
                     GKMFPPEYRNSIFIAQHGSWNRSKKSGYRVMLVTLNKDGKTVDKYEEFATGWMENERA
                     WGRPVDVLVMPDGALLVSDDTANRVYRISYVK"
     gene            complement(8331..>9914)
                     /locus_tag="VN496_07595"
     CDS             complement(8331..>9914)
                     /locus_tag="VN496_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXF16852.1"
                     /translation="FECFIDAVAAHGRGAKIMEDQADQWHTYGEILRMSLALSRLLRR
                     HTSDRENIGVLLPNMVSAVAVVMALSATRRVPAIFNYSAGPLAVECARVAAGIRTVVT
                     SRRFIEQAHLHSLLDALPGCAIVYVEDLRAGFRFADKLWLMAFAMRFPRLAITKQSTL
                     DPAVILFTSGSEDRPKGVVLSHHAVVANIAQIRAVFDFSPSDKVLNPLPLYHAYSFTA
                     GVMLPLITGTRVNLFISPLRYRAIPEIAYRQDCTILFGTSTFLSFYARYANPMDFSRL
                     RHVISGGEKLSPEVARVWMEKFGVRIMEGYGSTECAPVICLATPAAFRRGAVGAFLPG
                     VEYQIEPMDGVDNGGVLHVRGPNLMLGYYRYQNPGVIEAPKSTLGEGWYDTGDVVDVD
                     HDGLVTISGRVKRFAKIAGEMVSLDMIEHVAREASPDFHHAAVLNVEDFGGETTVLFT
                     TDVRLTRGRLLEAAKQLGTHDLSVARRLIPLKELPVLASGKIDYILLKALTEGDDVKR
                     LLALAMVQTVVDRETPPEQ"
BASE COUNT         2186 a         2828 c         2809 g         2091 t
ORIGIN      
        1 aacttccgcg gctgcatgat gcgcggcggc gtaatggccg tatgcggcaa catcgagtgt
       61 tccgggaagc ctgccatgac ctcgaaagcc agggccgcat cttccactgt gcgggtgatg
      121 ggcccgatat gatcgagcga ccaggccaac gggtagactc cttcgcgggg aacggcatcg
      181 taggtcggtt tgaacccgac aatcccgcaa cacgcggctg gcagacgaat cgatccaccg
      241 gtgcaggaac cgactgcgac ggccgcaagt tccgcagcaa ctgcggcagc cgatccgcca
      301 ctcgaaccgc ccggtatgta gccgggataa cgcggattct gcacgtgacc aaaatgcgga
      361 ttggcgctgc taatgccaaa agccagctcg tgaagattcg tggtaccgat aattagcgct
      421 ccggcagccc gcagccttgc cactactggc gcatcgcgct cctgcacttt gccttcaata
      481 gcccgggtac cggccgtaag cggataacct ctgactgaca tgagatcctt cactcctacc
      541 ggtacgccgg aaagcggccc gggatcttca ccacgccgca gacgatgggc cagaacctgc
      601 gcctcctcca tgaccaatga tggattcacc gtaatgaagg cattgaggtg tcgatgcgca
      661 tcgatgcggc cgagaaacag ctcagcgagt ttcaccggcg acagcgcgtg gtggcggacc
      721 tgatgggcga tctgcgccgc actggcggat accagtccgc cagccggcgc agaggaaaca
      781 tatcggcccg cctcaaactg ttccgttacg gttgcttttg gcgccgatag gaccggcgct
      841 tccgcaacag ccgcttcaaa aacctgttct atgtcgccga ggccaaaggc aaagcgttgc
      901 tcccagtccg gcaaccgttc gggatttgcg taacggcgaa aagcggaaac cgctcgcaga
      961 aattcagtca gaggacgtgg agacataagg gcggcacgcg gtttttgtta gatctgctag
     1021 tttcccgcat ttttcgattc gacgtatttc caatcccggc gtccagacga acaggttccg
     1081 gatgccctat ccagactatg gcaaaagccg ctgagtcggt cgagtagata gatgcttgca
     1141 acggagccgt cgtggacagc gtaaccgctg tttcgcttgt aacggcatac gcggtacact
     1201 gccgatgtaa tctcactgcg atttccgtga cgtctccgac cctaccctca aacgagcgcc
     1261 cgcaaccgcg agagcgtttt ctgctttatc ttgtccctgg actcgccgtc ctgctggtca
     1321 tcaccctttt cgcgctggca tttttcatgc tgagcaaatc acgcgagaat gctttgcgcg
     1381 atggagagcg gaccagcgcc ttccttgccc aaatcattga ggagcgcacc gggcggctgt
     1441 tgcagtcggt cgatcaagcg ctggggagca ccgtctacgc ctggcagcga agtccggcgc
     1501 tgcgagatcc gtcgggcttc gctttgcatg cgttacttgc tgaaagagtt gcgcaactgc
     1561 ctaacgcccg tacattgttc gtgctcgacg cgcgtggaat tctgctgcag gactcggata
     1621 tgcggtccgc agcacacatt gatttgtctg atcgtgacta cttcagctgg catcgtgcgc
     1681 acacagggaa tcgactctat gtcagtcgac cagcggagca gagcaacggc ggatggttta
     1741 tcgttgccac ccggcgactt gaagacacag aaggccattt cgccggcgcg gtaggggtag
     1801 cgcttgagcc cgcggccttc aaaacacttc ttgaccggct cgacgtggga caagccggta
     1861 ccagcgcgct gctgcacgag caaggagaat tgatcgcacg cgcccctgaa gcaccgaaat
     1921 ggttgggcgt tcccggcaaa gcgactccgg ggcaggattc acgaacctat cggaaggtca
     1981 gtgaagtaga tggcgtgttg cggatctatg gcgtgcgtcc ggtggccggc acaccattaa
     2041 tcgtgcaagt cggcctaagc gaagacgaac tgctggcagg ctggcggcac caactcaacg
     2101 tggcggcggc gatattggcg gcctttacgc tggcgactgt tctgctgacc tggttgctgg
     2161 tccaggggct catgcgccgc aataccttgg cgcgctcggt gggccgcaat gagcgcatgc
     2221 tccgacaggt gtttgacacc ttacccgtcg gcgttcgggt ggcggacaga gcagggcacg
     2281 tcgttctgag caacgccgcc agtggacgga tttggggcga tggtgagaaa gacctacatt
     2341 atttcactga ccgaaaaaaa gaactgccac agaccgccga tcgcggaatg gcgctcaccc
     2401 tgacaagagg cgcatcgtca cgtaatgagg tactggacat cgaatgttcg gatggcagcc
     2461 ggaaaaccgt attgcattca accgcaccga tcgtatcggc tgaaggggag atcgtcggtg
     2521 gcgtggcggt caatgaagac attaccgagc agcgggcgct ggtggacacg ctgcgcgaaa
     2581 gtgaagcgcg ctatcaggca ttgttcgagc acagcattga tgcggtattg ttgatgcgct
     2641 ctgatggccg gatcctgtcg gcaaacgagc aggcgtgccg gcttctgggc tataccgaaa
     2701 gtgaattacg caaactcgga cgtgaaggcc tgaccgagcc cgatgatccg cgtgtggaag
     2761 ccatggtgga agaacaccgg cgcgaaggtg gtgcccgcgg tgaactcaag atgcgccgca
     2821 aggatggagg ctgggttccg gtagaggtct cctcgactac cttccgtgac cggtcgggca
     2881 agctgtgcgc cagtctcatc atccgcgaca ttacggatcg caagcgtgcc gaggaacaca
     2941 ttgaatatct cgcctatcac gatgagctga ctggaattcc gaatcgggcg catttccagc
     3001 gcgcatttga gcaggcaatc acgatccacc agcgtcaggg cctgaaatcc gcgctcatgg
     3061 tggtggacct tgaccgtttc aagaatatca acgacatcat tggccacgaa gccggtgatc
     3121 agttgctgaa gcaaatcgcg gcgcgtctac gtacctgcct tcgcgatagc gatgtcctcg
     3181 cgcggctggg tggagacgaa ttcgtcatcc tgatgcagga cgcggccagt gtcgaagcag
     3241 tcagcgccgt ggcaaacaag atacttgagg caacgtcgcg tccgctaacg atcgacgagc
     3301 aggaattcgt cattacagca agtatcggga tcagtaccag tccacatgac ggcaccgatt
     3361 tgcagacatt gctgcgcaat agcgacgtcg ccatgtatcg ggcaaaggaa tcgggcaaga
     3421 acgggttcca gtatttctcc ccagacatgg atgcgcatgg gcgggagcgg ctttccatag
     3481 agtcttctct ggggcgcgcc ctggagcggc aggaatttat gcttcagttc cagccgaaga
     3541 tgcaggtgtc ctcgcgtaac gtggcaggaa tggaagcctt ggtgcgctgg caaaatccgg
     3601 agaaagggct gatgcagccg agcgctttca tcggcatcgc ggaagaaaca gggatgatcg
     3661 tgccaatcgg cgattgggtg ctgaccgagg cctgccgcca ggggcaggca ttgcgcgatg
     3721 ccggccacgc cggtttgcgc gtggcagtaa atctgtccgt gcgccagctc tatgacgacg
     3781 gcctggcaat gcgagtcagc gacgcgctta agagaaccgg gttcccggcc gaaaatctgg
     3841 aactggaaat tactgagagc atggtcatgc gcgatgccga gggcgcaatc aaggtgctgc
     3901 aggcccttcg cgacactggc gtgcgcattg ccatcgacga tttcggtacg ggttattcgt
     3961 cgctcgccta tttgaagcgc ttcccgatcg attgcgtaaa gatagaccgt tcattcatac
     4021 gggatttgcc cgacgatcgc gatgacgctt ctatcacgcg cagcatcatt gccatggcac
     4081 ataacatgaa tctcgaagta gtcgccgaag gcgtagagac cgtgccgcaa cttgaattcc
     4141 tgcacgccta tggttgcgat gaaattcagg gctttttatt cagcaagccg ctaggcgccg
     4201 aggcattcga gcagtttctg gacggacagg cttctcctcc gcctgctttt catttgcaag
     4261 cctgatctga ccggcttacc caagctcttc ggcagcgact ataatttcct gcatcgcatc
     4321 acttagcgga gagcgccatg tctgccaccg cagctgctca acacgtccat gtcgaaccga
     4381 tcctgctgcg caaagatgtg ggcggtatcg caaccatcac gttgaaccgc cccaagcaat
     4441 acaactcgct cagcgaagaa ctgctgacag agcttcagtc gatgttggac gtcgttggca
     4501 aggacacatc ggtgcgggtg gtcgtgatag cgggctccgg tccggccttc tgcgcgggcc
     4561 acgacctcaa gcaaatgcgc gccgatccac gcaagagtta ttacgacaaa ctgtttgcgc
     4621 aatgcagcga cgtcatgatg aagctattgc atcttccgca accggtcatc gctcgggtac
     4681 acggaattgc aactgcagcg ggttgccagc tggttgcgca atgtgatctt gcggtcgcct
     4741 gcgatacggc ccgatttgcc gtatccggta ttaatgtcgg gctgttctgt tcgacaccca
     4801 gcgtagcact aggccgaaat gtatcgcgca agcaagccat ggaaatgctg cttaccggcg
     4861 atttcatcga tgcggcgacc gccaagcaac gtggtcttgt gaatcgcgtc gtgccagaag
     4921 cgcagctcga tactgaagtc aaagcgcttg cggaagcgat catcgccaaa tctgccgtgg
     4981 ccgtgaccac gggcaagaaa atgttctata agcagatgga gatggggatg caagccgcct
     5041 acgaatacgc ggccgaagtc atggcgtgca acatgatggc agaagacgtc ggggaaggaa
     5101 tcgatgcctt catgcaaaag cgcccgccgc gctggcatga caaataaatc ttagcgcgtc
     5161 atttattcgc aagcgtcagc atcatttcgt tcaggcgttt gacgaaggac gcaggatcgt
     5221 ccagttgccc gccttcggca agcagggcct gatcgaacag gatatggctc cagtccccaa
     5281 aacgcccctt gtcggtttcg tatttcagga gttgcaccag gggatggtgg ggattgatct
     5341 ccagaacggg cttactgccc ggcacggttt ggcctgcggc cttcagcatg cgctcaagat
     5401 taccgctcat cccatgctgg tctgccacca ggcaagccgg cgaagatgta agccttagtg
     5461 ttacacgcac ctccttaacg cgatcgccca ggcttttgcc gatgttctcg acgagttcct
     5521 tgttctcgcc agcctccttc tcctgctctt tcttgtcttc atcggccagc ttgcccaaat
     5581 ccaggtcacc tttagcaacc gatgccatct gcttaccttc gaattccgtc agattcgacc
     5641 caacccattc gtcgacacgg tccgatagca gcaatacttc gatgcctttc ttgcgaaaaa
     5701 tctcaaggtg cggactgttc ttcgcagcgg caaagctgtc agcagtgatg tagtaaattt
     5761 tcgcctggcc gtccttcatg cgcgccacat agtccgacag gctaacgctc tgttcctcac
     5821 tgtcggcatg tgttgaggcg aagcgcagaa gtctggcgat acgctccttg ttggaccaat
     5881 cctcgcccac gccttccttc agcactcgcc cgaattcctt ccagcaagtt gcatatttct
     5941 cagactgagt ctgtgcgagg tcttccagca ggcccagcac cttattgacg caacctttgc
     6001 ggatggtttc gacgtctttt gattcctgca gaatttcgcg ggagacattc aacggcagat
     6061 cgttggagtc caccactccg cgtacgaacc gcaaataagc cggcaggagc tgttcagcat
     6121 cgtccatgat gaatacccgc cgcacatata acttgatgcc gtgacgatgt tcgcggtccc
     6181 acagatcaaa cggggcacgc gcgggtatgt acagcagctg cgtgtattct gattttcctt
     6241 ctacctttgc atgcacccag gcaagcggtt cttcaaaatc atgacttacg tgtttgtaga
     6301 actcgcggta ttgttgttcc gtgatctcgg attttggccg agcccataag gcggaagcct
     6361 ggttgacttt ctccagttcg tcacgagtaa catgctcagc cttgtccttg tcccaatcct
     6421 gcttgttcat caggatcgga agggagatat ggtcggaata cttgcggatg attgattgga
     6481 gacgaaagcc ttccagcaat tcgtcctcgc cctcgcgcaa atgcaaggta acttccgttc
     6541 cgcgcgatga tcgttcaacg atctctatgg tgtaatcccc ttctccgccc gattcccaac
     6601 gcaccccatg ctccttgccg agcccggcac gccgggtagt caacgtgacc ttgtcggcaa
     6661 caatgaaaca tgaatagaag cccacgccga attgcccgat caggctcgcg tcttttgcct
     6721 ggtcacccgt gagccgcccg aaaaattccc gggtgccgga tttggcgatg gtcccgatgt
     6781 tgtctatgac ttcctgacgc gacatgccaa tgccattgtc agataccgtg atggtccgtg
     6841 cagcacgatc aagggagact cgaattttga gatcggggtc atcttcgaac aggccgggat
     6901 cggtcaacgc ctcgaaacgc agcttgtcgc aggcatcgga cccattcgaa atcagttccc
     6961 gcaggaagat ctccttgttg ctgtacaacg agtgaatcat caactgcaac agctgcttga
     7021 cttcggtttg aaagccgagt gtttcccgag tggcggcttc gcccataacg ttttctcctg
     7081 taactcatag caataatgcc agatagggac gctatcttat gcattcaagg taccggccgg
     7141 aaccccgcgt tggaatacgc tgtctgcttt ccaacatcac cggatttttg aaggagacaa
     7201 gcccatgagc ccgcgcgtcg cctacctgct cttcgccctg gcgttctttg ccggcactgt
     7261 ggccgctacg ccgccactgg atcacatcaa gctcccggct gggttcaaaa tcgacgtgta
     7321 cgccgaagtc gagaatgccc gctcgctaac gctcggtgat aacggaacgg tgtttgtcgg
     7381 cacgcgcacg gacaaggtct acgccttggt ggattccaac cgtgatggca aagcagacaa
     7441 agtcgtcacg gtggcgcaag gcctgaacgc cccgaacggc gtggcttttg gagatggagc
     7501 gctttacgtc gccgagatca atcgaatact gcgctttgac aatatcgaag ccaatcttgc
     7561 gaaaccgccg aaaccggcgc tggtgttcga caagcttccg cgcgaatctc accatggttg
     7621 gaaatttatc gcttttgggc ccgatggctg gctatatgtg ccggtgggtg caccatgcaa
     7681 tatctgtgaa gcgaacccgg acgtgtacgc cgcaattcac cgcatgaagc cggatggcag
     7741 tcaatttcag gtttttgcac gcggcatccg taacaccgtg gggtttgact gggatccccg
     7801 cacgcaggaa ctatggttca acgaccatgg ccgcgacatg atgggtgatg acatgccatc
     7861 ttgcgaactc aaccacgcgc cgaaagcggg catgcatttc ggttttccgt attgccatca
     7921 gggcgatacg cctgacccgg aatacggcgc taagcgaaac tgcagcgaat ttgtccctcc
     7981 ggcgctaaag cagggaggtc atgtagcgcc taacggactg cgtttctata ccggcaaaat
     8041 gtttccgccc gagtaccgga atagtatttt tattgcgcag catgggtcct ggaatcgcag
     8101 caagaaaagc ggctatcgag tgatgctggt cactttaaac aaggacggta aaaccgtgga
     8161 taagtatgaa gaattcgcaa ccggctggat ggaaaacgaa agggcctggg gccgcccggt
     8221 cgatgtactc gtcatgcccg atggagcttt gctggtttca gacgataccg cgaatcgagt
     8281 atatcgaatc agctacgtaa aataggcgac gccgctcagt gcgccgggcg tcattgctcg
     8341 ggaggtgttt cgcgatctac cactgtctgg accatcgcca gggcgagcaa gcgcttcaca
     8401 tcatctcctt cagtaagcgc cttcagtaag atgtaatcaa ttttcccgga cgcaagcaca
     8461 ggcagctctt taagcggaat cagccggcga gccacggaga gatcatgggt tcccaactgc
     8521 tttgcagcct ccagcaagcg accacgggta agccgaacgt cagtggtgaa cagcacagtc
     8581 gtttcgccac caaagtcttc aacattcaat acggccgcat ggtgaaagtc aggagaagct
     8641 tcgcgcgcca cgtgctcgat catatcgagt gacaccatct ctccagcgat tttggcgaat
     8701 cgtttgacgc ggccggagat agtcaccagg ccgtcatgat ccacatccac cacgtccccg
     8761 gtgtcgtacc atccctcacc aagtgtggac ttcggcgctt caatcacgcc aggattctga
     8821 taccggtaat aaccgagcat cagatttgga ccgcgcacat gcagcacacc gccattgtcc
     8881 acgccgtcca tgggttcaat ctgatattca actccgggca ggaaagcgcc aaccgcgcct
     8941 cggcgaaagg ctgcgggtgt ggcaaggcag ataacaggcg cgcattcggt ggacccgtag
     9001 ccttccataa tccgcacgcc aaatttctcc atccagaccc tcgcaacttc cggcgaaagt
     9061 ttttcaccgc ccgatatgac atggcgcagt ctgctgaaat ccatcggatt cgcgtaacgg
     9121 gcgtaaaacg acaggaaagt actagtcccg aacaggatag tgcaatcctg ccggtatgcg
     9181 atttccggaa tcgcgcggta acgcagcggc gaaatgaata aattaacccg ggtaccagtg
     9241 atgagcggaa gcatgacgcc cgcggtgaag ctataggcgt ggtacagcgg tagtggattc
     9301 agcactttgt cgcttgggga aaaatcgaac actgcgcgga tttgcgcaat atttgcgacg
     9361 acggcatggt gcgaaagtac cacgcctttg ggccggtcct cggagcccga cgtgaacagg
     9421 atgactgccg gatctagcgt ggactgtttg gtaatggcca gccggggaaa gcgcattgca
     9481 aaagccatga gccaaagctt gtcggcaaat cggaacccgg cgcgcaaatc ttcgacatag
     9541 acaatcgcgc acccgggtaa ggcatccagc aatgaatgca gatgcgcctg ctcaatgaat
     9601 ctgcgcgacg tgaccacggt acggattccg gccgctaccc tggcacactc gactgccagt
     9661 ggcccagccg aataattaaa tatcgcggga acgcgcctcg tcgccgacaa cgccattacc
     9721 accgcaaccg cactgaccat gttgggcagc agtacaccaa tgttttcccg gtcgctggta
     9781 tgccgccgca acaggcgcga cagtgccaag ctcatgcgca ggatttctcc ataggtgtgc
     9841 cattggtcgg cctggtcttc catgatcttc gcgccacggc catgtgcagc aaccgcgtca
     9901 atgaaacatt cgaa
//
LOCUS       DATMZQ010000232         7421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1905428, whole genome shotgun sequence.
ACCESSION   DATMZQ010000232 DATMZQ010000000
VERSION     DATMZQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905428"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VN496_07600"
     CDS             <1..314
                     /locus_tag="VN496_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF16853.1"
                     /translation="WDLLPWMRERHIPVIAYSPIEQARLLSNPKLVDFARRHAMTPVQ
                     VALGWLLAKPDVIAIPKAGNRAHVKENASALEIALSAEQLVELDKAFPPPKKSVPLEM
                     L"
     gene            418..930
                     /locus_tag="VN496_07605"
     CDS             418..930
                     /locus_tag="VN496_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXF16854.1"
                     /translation="MATHWSQQLHELLRQQNVRQVSFVPDAGHTSLIKLCEADSAMRA
                     VRLTTEEEGVALAVGAWLGGDKSVLLMQSSGVGNCINMLSMLSVCQIPLTMLVTMRGD
                     WAEFNPWQVPMGRAAKAVLEEMGVLVNRADREEDIVPLAEGALRIAYNTLRPTAVLIS
                     QRLLGAKTFD"
     gene            941..1558
                     /locus_tag="VN496_07610"
     CDS             941..1558
                     /locus_tag="VN496_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXF16855.1"
                     /translation="MTTTATMQPIQMLERRTVVKTLLAERGGLLVIAGLGAPAWDATD
                     AGDHPLNFPLWGGMGGAATIGLGLALAQPERRVLVLTGDGELLMGMGSLATIGVQGPP
                     NLSLVVLDNEHYGETGMQPSHTKFGIDLAACAKACGFARSNMVTTMQQVSTLREEIHQ
                     ATGPLFAQIKIAPDKLPLVLPPRDGTHLKNRFREALIGQQAHFRR"
     gene            complement(1694..1996)
                     /locus_tag="VN496_07615"
     CDS             complement(1694..1996)
                     /locus_tag="VN496_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16856.1"
                     /translation="MDRRALLEQSIMVLAQRAGVDRPVFHVISVSQDSAGIFLMQTRW
                     PDGQAIYEAIARDVDAFPKDHYTTCVALLPEVVDQIIRAAREERPAVASVLAEERL"
     gene            complement(2108..2371)
                     /locus_tag="VN496_07620"
     CDS             complement(2108..2371)
                     /locus_tag="VN496_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXF16857.1"
                     /translation="MIRVFIPSPLFSYTGGQKQIEAAGESLAALLQTLDRRYPGMRHR
                     IVDEQDCVRKHIRFFINGEIATRLDQTLTSGDEVMIVAALSGG"
     gene            complement(2368..3483)
                     /locus_tag="VN496_07625"
     CDS             complement(2368..3483)
                     /locus_tag="VN496_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXF16858.1"
                     /translation="MTDAVTLLVGTRKGALIYRGDQARNSWKLEGPYYLGNIVNHMVA
                     DPRDGRTILMAAKTGHLGPTVFRSSDGGKHWAEAQQPPVFRKAAQGEEGRVVESVFWL
                     SAGHASEPGVWYAGTSPPGLFKSADGGVTWAEVSGFNDVLYQKIKHAIVPGPDGAQVH
                     SVCVDSSDARHLYLGISIGGVFESRDAGNSWHPLNKGVAADFLPDPNTEYGHDPHTVA
                     VHPRHPERLYQQNHCGIYRLDRPQQKWERIGVNMPKDVGDIGFPIVLHPRDPDCIWVF
                     PMDGTEVWPRTSPGGKPAVFRARDGGASWQRLDAGMPTQHAWWTVKRQAMCADTHDPV
                     GLYFGTTSGELWASRDEGEHWSRIAEHLPHIYSVTVA"
     gene            complement(3494..4234)
                     /locus_tag="VN496_07630"
     CDS             complement(3494..4234)
                     /locus_tag="VN496_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HXF16859.1"
                     /translation="MTEAKHVLVPTAPFALLPTCPEHRLSRRQVLKMSLALGAVGFLS
                     QGIAAEKEGVTGLDFNGPMPTPDQILGPFYPVQKPADGGVDLTRLKGRAGQAQGEIIY
                     VTGRVLNLRGDPCPGVRLEIWQANGAGRYTHPHDRNPAAIDPNFDGYANVTTDAEGHY
                     RYRTVKPGAYPVVADYWRPPHIHYDITGRVNRLITQMYFADEPLNRKDPLLQQSWAKE
                     SLIAKILPASPEEPNARLVVWDIVLIQG"
     gene            complement(4267..5181)
                     /locus_tag="VN496_07635"
     CDS             complement(4267..5181)
                     /locus_tag="VN496_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF16860.1"
                     /translation="MTATAVRAEIDQDTYASRARYLINENGFAFSWPAVPMRQFLAER
                     DRAFSAECPTGLIALDSSADLHTQYPATTPTLLCRYVKLRATEQMRTAFAASGEIFYV
                     MSGTGESRNGPDVVDWSSGDVFCFPGGGETMHRAGGADCLLFAVTNEPLLAFERLRPP
                     LPGQAVVETTHWPAAEIERHFLEVWRRPVSANATGNSVQFSTTALQPSSNTIPSINTA
                     INTLEPGCDQRPHRHNGVAVTLAIQGEGVYSLIDSKRVDWSTGAAQITPATLLHSHHS
                     RGTQRMRSFVIQDEGLHHYTRTPGFSFD"
     gene            complement(5246..6721)
                     /locus_tag="VN496_07640"
     CDS             complement(5246..6721)
                     /locus_tag="VN496_07640"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF16861.1"
                     /translation="MSDDMLSLSAAALTALYRSKQLSPVEVSRAALERIARLQPLYNA
                     FVMVDEVRAIKDARASEARWRKGAPAGSVDGVPSTVKDLIVTQGWPTLRGSRTIDPKQ
                     PWTEDAPPVARMKEQGAVFLGKTTTPEFGWKGVTDSPLTGVTVNPWNPGMTPGGSSGG
                     AAVAAALGMGTLHLATDGGGSIRIPAGFCGLFGFKPTFGIVPVHPHSPAGTLWHQGPI
                     VRTVEDAALMLSVIARPDARDWYQAPPLNIDYSAGLNDGVKGLRIAYSRTLGYARVDP
                     DVADKVDAGAKVFESLGATVEDLDLKLEDPIAIMQPLWAVALALAIEPMNEKQRALLD
                     PPLLVLAQSGFGVDALAYRKLERAREAFARRMCQLHQTYDLLLTPQLAVTAFEAGHEV
                     PPGSGRTRWWEWSPFTYPFNLSQQPAAAVPCGFTDQGLPVSLQLVGNKFADALVLRAA
                     RAYEHAQPFIMPGAHPAGTAKKLRDSTAPRPAVRGRRTALR"
     gene            complement(6822..>7421)
                     /locus_tag="VN496_07645"
     CDS             complement(6822..>7421)
                     /locus_tag="VN496_07645"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXF16862.1"
                     /translation="PWPKEGSWPLYESATVGGALRLANCLRVAAGKSGYGTKRLPANT
                     GMGIAVTAAEERQSPTWVAGVAEVAVDPASGAFSINRITIAIDPGIAVNPANIEAQVR
                     GSALWGASQIMSEKLTLKNGSFEQSNFHDYQPIRLAQVPQIDVTIIQSSHHPSGVGEP
                     ASTIVAPAVANAIANAVGARVRHMPITAEAVLAAMKGKA"
BASE COUNT         1453 a         2267 c         2223 g         1478 t
ORIGIN      
        1 aatgggattt gctgccctgg atgcgcgaac ggcacattcc ggtcatagcc tattcgccga
       61 tcgaacaagc acgattgctc agtaatccaa aactcgtcga tttcgcgcgc cgccacgcca
      121 tgacgccggt acaagtcgcg ctgggctggt tactggcaaa accggatgtc atcgcaattc
      181 cgaaagccgg caatcgcgcc catgtaaagg agaacgccag tgcacttgaa attgcactat
      241 ccgcggaaca acttgtcgag ctggataaag cgttcccgcc acccaaaaaa tcagtgccgc
      301 tggaaatgct gtaaacaagc gcctcgattt actgattgct gccgcgccgg ccttgccgcg
      361 cgcaagcgac tgtggccaaa tcgcgcttct accagttcaa cgatttttgc aatgctcatg
      421 gcaacccatt ggagccagca actgcacgaa ctactccggc aacagaatgt gcgccaggta
      481 agctttgtcc cggatgccgg ccatacgtcg ctcatcaagc tctgcgaagc tgactctgcc
      541 atgcgcgcgg tgcggctcac aaccgaagaa gaaggtgtcg cgctcgccgt cggtgcgtgg
      601 ctgggagggg acaagagcgt gctgctcatg cagtcgagcg gagtcggtaa ttgcatcaac
      661 atgctttcga tgctttccgt ttgccagata ccgctgacga tgttggtcac gatgcgcggc
      721 gactgggcgg aattcaaccc gtggcaggta ccgatgggtc gtgcagcaaa agcagtcctc
      781 gaagaaatgg gcgtactggt gaatcgcgcg gaccgggaag aagacatcgt gccgctcgcc
      841 gaaggcgcat tacgtatcgc ctataacact ttgcgtccga ctgcagttct catcagccag
      901 cgccttctcg gcgccaagac gttcgattga ctgaaatgaa gtgaccacga cagctaccat
      961 gcagccaatc cagatgctgg aacgccgaac tgtcgtcaag acgctgcttg cggagcgcgg
     1021 cggactgctg gttatcgctg gactcggcgc gcccgcctgg gacgcgacgg atgccggaga
     1081 ccatccgctt aattttccgc tgtggggtgg catgggcggc gctgccacga tcggactcgg
     1141 gcttgcactt gcccaacccg agcggcgcgt gctcgtgctg actggtgatg gcgaactgct
     1201 gatggggatg ggctcgctgg caacaattgg cgtgcaaggc ccgcccaact tgtctctcgt
     1261 cgtgctcgac aatgagcact acggtgaaac cggcatgcag ccttcccata cgaagttcgg
     1321 cattgatctg gcggcgtgtg cgaaggcgtg tgggttcgcg cgatcaaata tggtcacaac
     1381 catgcaacag gtcagcacgc tacgtgagga gatccatcaa gccacgggac cattgttcgc
     1441 gcagatcaag attgcaccgg acaagctgcc gctggtattg cctccgcgcg atggcaccca
     1501 tctgaagaat cgcttcaggg aagctttgat cggccagcaa gcgcatttcc ggcgctaggg
     1561 cggcgacgat gtttcttgcc gccgccagct gtcgatgcag accgtacaaa acgcgaacaa
     1621 cgatcgctgt cggtatagcg gctggcccag attcgcggca agccggacat gcggcgtctt
     1681 cctgtcgtcg atgtcaaaga cgctcctctg ccaggaccga tgcaactgcc ggccgctctt
     1741 cgcgcgctgc gcgaatgatc tgatcgacga cttcagggag gagcgccacg cacgtggtgt
     1801 agtgatcttt gggaaacgca tcgacatcgc gcgcaatcgc ttcgtatatt gcctgaccat
     1861 ccggccagcg ggtctgcatg aggaaaatgc ccgcggaatc ctgcgatacg ctgatgacgt
     1921 gaaacaccgg cctgtcgacg cccgcgcgct gcgcaagcac catgatggat tgctccagca
     1981 aagctcttct gtccattggc agtccccttg atccgctgat ctgacaggat cactctagcg
     2041 aagttccaag acagtttcgc gacagcgcac gtccagctat tcattgaacc agcgacgaaa
     2101 aatttcttca gccgccgctg agggcagcca cgatcatgac ttcatcgccg gatgtcagtg
     2161 tctggtccag acgcgtggcg atttcgccgt tgatgaagaa gcgaatgtgt ttgcgcacgc
     2221 agtcctgctc atcaacgatg cgatggcgca tgccgggata gcgcctgtcg agggtttgca
     2281 gcaacgccgc aagcgattcc cccgcggctt cgatttgctt ttggccaccg gtataggaaa
     2341 agagcgggct cgggataaag acgcgaatca cgcaaccgtt acggaataga tgtgtggaag
     2401 atgttccgcg atgcgcgacc agtgttcgcc ttcgtcccgg ctggcccaca gttcaccgct
     2461 ggtagtgcca aaatacaaac caaccggatc atgagtgtca gcgcacatcg cctggcgttt
     2521 cactgtccac catgcatgct gagtcggcat gcccgcatcc agccgttgcc atgaggcgcc
     2581 gccatcgcgt gcgcggaaca cggccggctt accgccggga ctggtgcgcg gccacacttc
     2641 agtgccatcc atcggaaaca cccagatgca atccggatca cgcggatgca gcacgattgg
     2701 gaagccgatg tcaccgacat ctttgggcat atttacgccg attctttccc acttctgctg
     2761 cggacgatcc agccgataga tgccgcaatg gttctgctga tagaggcgtt caggatggcg
     2821 gggatgtact gcaacagtat gcggatcgtg cccgtactcc gtattcgggt ccggcaaaaa
     2881 atccgccgcg acgcccttgt tcagtggatg ccaggaattg ccggcgtccc tgctttcgaa
     2941 cacaccacca atggaaatac cgagatagag atgacgcgcg tcgctggaat cgacgcacac
     3001 ggaatgcact tgcgcgccgt ccggaccggg aacgatcgca tgcttgatct tctggtagag
     3061 gacatcgttg aagccggaca cttcagccca ggtaacgccg ccgtcggcgg acttgaaaag
     3121 tcctggcggt gaagtgcctg cataccaaac cccgggttcc gaagcatgtc cggccgaaag
     3181 ccagaatacg ctttccacca cgcgcccttc ttcaccttgc gccgctttcc tgaataccgg
     3241 gggttgttgc gcttcggccc agtgctttcc gccgtcgctc gaacggaaca ctgtcggtcc
     3301 cagatggccg gttttagcag ccatcaggat cgtgcgtcca tcacgcggat cggcaaccat
     3361 gtggttgacg atgttgccca gataatatgg gccttcaagt ttccaggagt tcctggcctg
     3421 gtcgccgcga tagatgagtg cgcccttgcg tgttcccacc agtaaggtca cggcatcggt
     3481 catggccggt cgcttatccc tgtatcagca cgatgtccca gaccaccagc cgcgcattgg
     3541 gctcttcagg tgacgccggc aaaatctttg cgatgaggct ctctttcgcc caagactgct
     3601 gcagcagcgg atctttgcgg ttgagcggct catccgcaaa gtacatctgc gtaatgagcc
     3661 ggttcacccg gccggtgatg tcgtaatgaa tatgcggcgg acgccagtaa tcggcaacaa
     3721 ccgggtaggc cccaggcttt acggtccggt aacgatagtg tccttccgcg tccgtcgtga
     3781 catttgcata gccgtcaaag ttgggatcga ttgctgccgg attgcggtca tgcggatgcg
     3841 tgtagcgccc ggcgccgttg gcttgccaga tttccaggcg cacgccggga cacgggtcac
     3901 cgcgcaggtt caataccctg ccggtcacat agatgatttc gccttgcgcc tgtccggcgc
     3961 ggcccttgag ccgcgtgaga tcaacgcctc cgtccgcagg cttttgcacc ggatagaagg
     4021 ggccaagaat ctgatcgggc gtcggcatcg gtccgttgaa gtccagaccc gtaacgccct
     4081 ccttttctgc cgcaattcct tgcgagagga agccgactgc tccgagggca agcgacatct
     4141 ttagaacttg ccgccgcgac agccggtgtt cgggacaggt cggtagaagc gcaaacggcg
     4201 cagtcggaac gagaacatgc tttgcttcag tcatttcttc tccaggaaaa ttcggctgct
     4261 gatttattaa tcgaagctga aacccggcgt gcgcgtgtag tgatgcaggc cctcgtcctg
     4321 aatgacgaac gagcgcatgc gctgcgtgcc ccggctgtga tgagagtgca acagcgttgc
     4381 cggtgtgatt tgggccgcgc cggtagacca gtcaacgcgc ttgctgtcaa tcagcgagta
     4441 gacgccttcg ccctggatcg ccagcgtgac cgcgacgcca ttgtgccgat gtggccgctg
     4501 atcgcagcca ggttcgagtg tgttgatggc ggtgttgatg gacgggatgg tattggaact
     4561 gggctgcagc gccgtggttg aaaactgcac ggaattgcca gtggcgttcg cactgaccgg
     4621 gcggcgccag acttccagga agtggcgctc gatctcggca gcgggccaat gcgtcgtctc
     4681 gactaccgcc tgtcccggga gtggcgggcg caagcgctcg aacgcgagca aaggttcgtt
     4741 ggtaaccgcg aacagcaggc agtctgcgcc gccggcgcga tgcatggtct caccgcctcc
     4801 gggaaagcag aatacgtcgc cgctgctcca gtccacaacg tccggtccgt tgcggctttc
     4861 tccagtgccg gacatgacgt aaaagatttc acccgacgcc gcgaatgccg tgcgcatctg
     4921 ttcagtcgcg cgcaacttca cataacggca cagcaacgtc ggcgttgtcg ccggatactg
     4981 tgtatgaaga tccgcggaac tgtcgagcgc aatcaatccc gttgggcatt cggcggaaaa
     5041 agcccgatcg cgttcagcca gaaactggcg catcggtacc gcgggccagg agaaggcgaa
     5101 accgttttcg tttatgagat aacgcgcgcg gctggcgtac gtgtcctgat cgatttcagc
     5161 gcgaactgcg gtggcggtca tgggcggctt ctcctgtatg gccgcctaat atactgctat
     5221 cggcctgact gcgccgcggg ctcgcttatc gcaatgcggt ccggcggccg cgcactgccg
     5281 gccgcggtgc cgtgctgtcc cttagttttt tcgccgtgcc ggcgggatgg gccccgggca
     5341 tgatgaacgg ctgcgcatgc tcgtaagcgc gcgcggcccg caaaaccagc gcgtcggcga
     5401 atttattgcc gactaattgc aatgaaaccg gcagaccctg atcggtaaat ccgcacggca
     5461 ctgcggcggc aggctgctgg gacaaattaa acggataggt gaagggagac cattcccacc
     5521 agcgggtgcg tcccgatcca ggcggtactt catgccctgc ttcgaatgcg gttaccgcaa
     5581 gctgcggcgt aagcaaaaga tcgtatgtct gatgcagctg gcacatgcgc ctggcaaagg
     5641 cttcccgcgc ccgctccagt ttgcggtagg caagcgcgtc gacgccgaat cccgactgcg
     5701 cgagcacaag cagcggcggg tccagcagcg cgcgctgctt ttcattcatc ggctcaatgg
     5761 caagcgcgag cgccacggcc cagagcggct gcatgattgc gatcgggtcc tcgagtttga
     5821 ggtcgaggtc ttcgaccgta gcccctagcg attcgaacac tttcgctccg gcgtcgacct
     5881 tgtccgccac atccggatcc acccgcgcat aacccaacgt gcggctgtaa gcgatgcgca
     5941 aacctttgac gccgtcgttc aagcctgcgc tgtaatcgat attcagtggc ggcgcctgat
     6001 accagtcgcg cgcatccggc cgcgcgatca cgctcagcat cagcgccgca tcctccactg
     6061 tgcggactat gggcccttga tgccataagg tgccggcggg cgaatgtgga tgtaccggca
     6121 cgataccaaa cgtgggcttg aatccgaaca acccgcagaa ccctgcggga atccggatcg
     6181 aaccgcctcc atcggtggca agatgcagcg tgcccatgcc cagcgccgca gcgactgcgg
     6241 cgccgccgct gctgccgccg ggcgtcatgc ctggattcca gggattcacg gtaacgcccg
     6301 tgagcggact atccgtcacc cccttccagc caaattcagg cgtagttgtt ttgccgagaa
     6361 acacggcgcc ctgttccttc atgcgcgcca ccggcggagc atcttcggtc cacggctgct
     6421 tggggtcgat cgtgcgtgag ccgcgcagcg tcggccagcc ctgcgtcacg atgagatcct
     6481 tcaccgtgct cgggacaccg tccacgcttc ccgcgggcgc gcccttgcgc cagcgcgctt
     6541 ccgaagcgcg cgcatccttg atggcacgga cttcgtccac catgacaaac gcgttataaa
     6601 ggggttgcag cctggcaatg cgttcgagcg cggctcggga cacttcgaca ggagacagtt
     6661 gcttgcttcg gtaaagcgcc gtcaatgcgg cggcggacag tgaaagcatg tcgtctgaca
     6721 tgttgctgct gctcctgctg tagtggccag cggccgattc cggccggaat cgcgggctcc
     6781 gtggctaaag accgccgcgg aacccggaca cacttatcgc ctcatgcctt gcctttcatc
     6841 gctgcgagca ccgcctcggc agtaatcggc atgtgcctca cgcgcgcacc gacagcattc
     6901 gcgatggcat tcgcaactgc cggcgccacg atagtggacg ccggttctcc gaccccgctt
     6961 ggatgatggc tcgactgaat aatggtgaca tcgatctgag gtacctgcgc cagccgtatc
     7021 ggctggtagt cgtggaagtt gctctgctcg aagctgccgt tcttcagcgt caatttctcc
     7081 gacatgatct ggcttgcacc ccacaacgcc gagccgcgga cctgcgcctc gatattggcc
     7141 ggattgaccg cgatgccggg atcgattgca atggtgatcc ggttgatgct gaaggcaccg
     7201 gacgctggat cgaccgcaac ttcggcaaca ccagccaccc aggttggact ctggcgttcc
     7261 tccgcagcgg tgacggcgat ccccattccg gtgttggccg gcagcctttt ggtaccataa
     7321 cccgatttgc ccgctgcgac gcgcagacaa ttcgcgagcc gcagcgcgcc gccgaccgta
     7381 gccgactcgt acagtggcca actgccctct tttggccagg g
//
LOCUS       DATMZQ010000233         6385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4233534, whole genome shotgun sequence.
ACCESSION   DATMZQ010000233 DATMZQ010000000
VERSION     DATMZQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4233534"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VN496_07650"
     CDS             <1..766
                     /locus_tag="VN496_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXF16863.1"
                     /translation="TAARFVQAGISHNVGVVIFLEDLTRVQQQAQQLKLASLGRLTAN
                     IAHEIRNPLSAINHATELLLEESALRGSPHGRMLQIIHDNSQRLDRMVQDVLKLNRRD
                     RAVHEHFDLGDYLETFAGEFCEIEKVPMDIIRVEMQIEATIYFDRSHLNQVMWNLCRN
                     ALRYCQKQRGSIIVNVRSGPRQGTVELSVIDDGTGIAESLRGHLFEPFFTTASSGTGL
                     GLYIAREICEANAATLDYVERPRGAQFSLVCRGVSA"
     gene            763..2127
                     /locus_tag="VN496_07655"
     CDS             763..2127
                     /locus_tag="VN496_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXF16864.1"
                     /translation="MKRKEQASGTPSRSVLIVDDEEDILELIELTLLRMGLDVERATT
                     VKTAIEKVQDGDYDLCLTDMRLPDGEGLELVQFISDNISDLPVAVITAHGNTENAVAA
                     LKAGAFDYLAKPVSLEQLRTLVKSALSLPQATAAKSSTEQAMLGDSAAIQQVRELIEK
                     LARSEAPVHITGESGSGKELAARLIHQKGARRTQPFVPVNCGAIPENLMESEFFGYRK
                     GAFTGADSDRDGFFQAAHGGTLFLDEVADLPLPMQVKLLRAIQEKKVRKVGATQEDPV
                     DVRIISATHQKLGECVEAGKFRHDLYYRLNVIELRMPALREMREDIPQFVVALLDKLA
                     RNAGSKKPEVAPEALEELKRYDFPGNVRELENVLERAMALCSEGKITAEDLQLTPEDL
                     PGQPVVSGAESKAPLQDYLDRVEKDAILEALEKTRYNRTAAAKVLGITFRSMRYRMER
                     LGLN"
     gene            2200..2769
                     /gene="ampD"
                     /locus_tag="VN496_07660"
     CDS             2200..2769
                     /gene="ampD"
                     /locus_tag="VN496_07660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HXF16865.1"
                     /translation="MTDTLAWALDPAGYLGAARHVPSPNCDDRPDGSSIELVVVHNIS
                     LPPGQFGGSGIIDLFTNNLDPNAHPYYGQIAEAKVSSHFLIRRDGELIQFVPCSRRAW
                     HAGQSEWRGRARCNDFSIGVELEGTDERDFDESQYATLARLTLVLLNSYPIKECTGHS
                     DIATPRGRKSDPGPHFEWPRFQAMLRERG"
     gene            3248..6112
                     /locus_tag="VN496_07665"
     CDS             3248..6112
                     /locus_tag="VN496_07665"
                     /inference="COORDINATES: protein motif:HMM:NF005544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HXF16866.1"
                     /translation="MQLGTNLTAPASVGNYSAENAGSDEKERYAQYKVIRRNGAVVSF
                     EPGKIAVAVTKAFLAINGGQGAASARVRELVEKLTEAVVNALMRRQPNGGTFHIEDIQ
                     DQVELSLMRSGEHEVARAYVLYREDRARERARIREARPASATPGINVVEAGQTRPLEM
                     ERLTELIKESCEGLGRAVDAGLIVQMTLKDLYDGVPMDEVRKCTILAARSLIEKDPGY
                     SYVTARLLLNSVRFEVLGEEVAQTEMHSRYAEYFPQFIRHGIAAGLLDERLGRFDLKS
                     LGAALDANRDQKFGYLGLQTLYDRYFLHISDRRIELPQAFFMRVAMGLAINEVEREAR
                     AIEFYNVLSSFDFMSSTPTLFNSGTQRSQLSSCYLTTVADDLEGIYEAIKENALLSKF
                     AGGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLET
                     WHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMEAGEWTLFSPADAPDLHE
                     KVGKEFEAAYCEYERRARTGELKLFKRVPALTIWRKMLTMLFETGHPWITFKDPCNIR
                     SPQQHVGMVHSSNLCTEITLNTSDTEIAVCNLGSVNLLAHMKDGKLDQEKLKDTVSTA
                     MRMLDNVIDINYYAVNKARNANFKHRPVGLGIMGFQDCLHEMRVPYGSREAMEFADRS
                     MEAVAYHAYWASTELADERGRYSTYDGSLWSKGILPQDSLQLLTEQRGGYVDVDKSSG
                     LNWEPLRARIRQFGMRNSNCLAIAPTATIANIVGVSASIEPTYQNLYVKSNLSGEFTV
                     TNEYLVHDLKKLSMWDEVMIADLKYFDGSLAKIDRIPQEVRSLYATAFEVDPVWVVEA
                     GSRRQKWIDQAQSLNIYMAGASGKKLDETYKLAWTSGLKTTYYLRTLGATSTEKSTVR
                     GGQLNAVPTDGGLSGAGTTQAAIEDAKFCAIDNPECEACQ"
BASE COUNT         1462 a         1716 c         1875 g         1332 t
ORIGIN      
        1 gactgccgcg cgttttgtgc aggccggtat cagtcacaac gtcggggttg tcatttttct
       61 ggaggacctt acgcgggtac aacagcaggc gcagcaactg aagctggcat cgctcggacg
      121 gctgaccgcg aatatcgcgc acgaaatccg caatccgctg tcggcgatca accatgcgac
      181 cgagttgctg ctggaagagt ccgctctgcg cggttcacca cacggccgga tgctgcaaat
      241 catccacgac aattcgcaac ggctcgaccg tatggtgcag gacgtcctta aactgaaccg
      301 gcgggaccgc gctgtacatg aacacttcga tctgggcgat tatctggaga ccttcgctgg
      361 cgaattctgc gaaatagaaa aagtaccgat ggatatcatt cgcgtcgaga tgcagatcga
      421 ggctaccatc tacttcgatc gaagccatct caaccaggtc atgtggaacc tgtgccgcaa
      481 tgcgctgcgc tattgccaga aacagcgagg aagcataatc gtgaacgtcc gctcgggacc
      541 gcggcaaggt acggtagaat tgagcgttat cgacgacggc acgggcatcg cggaatcgct
      601 gcgcggtcat cttttcgaac cgtttttcac tactgcgtcg agcgggacgg ggcttggcct
      661 ttatatagcg cgggaaatct gcgaggccaa cgcagcgact ctcgactatg ttgagcggcc
      721 gcgcggcgcg caattcagtc tggtctgtag aggagtgtca gcgtgaaacg caaagagcag
      781 gcttcaggaa caccatctcg ttcggtgctc attgtcgacg acgaagaaga cattctggag
      841 ctcatcgagc ttacgttgct gcgcatggga ctagacgtag agcgcgcgac tactgtaaaa
      901 accgccatcg agaaggtgca ggatggtgat tacgacctct gcctcacgga catgcggctt
      961 ccggacggtg aagggctgga actggtgcaa ttcatctccg ataacatatc cgatctgccg
     1021 gtcgcggtca tcaccgcgca tggcaatact gaaaatgccg ttgctgcgct gaaggctgga
     1081 gccttcgatt acctggctaa gccggtttca ctggaacaac tgcgcacgct ggtcaaatcc
     1141 gcgctttcgc ttccgcaggc tacagcagcg aagtcttcga ccgaacaagc gatgctcggt
     1201 gattcggcgg caatccagca ggtacgtgaa ctcatcgaaa agctggcgcg cagtgaagcg
     1261 cccgtgcaca ttaccggcga atccggcagc ggcaaggaat tggccgcccg gctcattcat
     1321 caaaaaggcg cccgccgcac ccagccattt gttccggtga actgtggtgc aattcccgaa
     1381 aacctgatgg agtcggaatt cttcggctat cgtaaaggtg catttaccgg cgctgacagc
     1441 gaccgcgacg ggttcttcca ggctgcgcac ggaggcacgc tgtttctcga cgaagttgcc
     1501 gatctcccgc tgccgatgca agtcaaactg ctgcgcgcaa tccaggaaaa aaaggtgcgc
     1561 aaggtgggcg cgacgcagga agatccggtc gacgtgcgta tcatcagcgc aacccatcaa
     1621 aaattgggcg aatgtgtgga agctggcaag ttcagacatg atttgtatta ccgtctgaat
     1681 gtaattgaac tgcgcatgcc tgcattgcgg gaaatgcggg aggacatacc gcagtttgtc
     1741 gtggcgctgc tggacaagct agcccgtaat gcgggttcca agaagccgga agtcgctcca
     1801 gaagcgctcg aagaactcaa gcggtacgat tttccgggaa acgtgcgtga attggagaac
     1861 gtgctagaga gggcaatggc tttatgcagc gaaggcaaga ttacggccga agatctgcag
     1921 cttacaccgg aggacttgcc tggtcagccc gtcgtctccg gcgcggaaag caaagcacct
     1981 ctgcaggatt atctcgatcg cgtggagaag gatgcaattc ttgaggccct ggaaaaaact
     2041 cgctacaacc gtactgcggc cgcgaaggtc cttggcatca catttcgatc catgcgctac
     2101 cggatggagc gtctggggct gaattgaggc ggggaagcgc gaccttctaa cccagtccgc
     2161 gttgcgtcgt ttcgcttacg cccccgttta cctttcaaaa tgacagacac gctggcatgg
     2221 gccctggatc ccgcgggata tctgggagcc gcgcgccatg tgccttctcc aaactgtgat
     2281 gatcgtccgg acggctcgtc catcgagctg gtagtggtgc ataacatcag cttacccccc
     2341 ggccagttcg gcggttccgg catcattgat ctttttacca acaatctcga tcccaatgca
     2401 catccgtact atggtcaaat cgccgaggcc aaggtttcca gccactttct gatacgtcga
     2461 gacggcgaat taatacaatt tgtgccttgc agtcgacgcg cgtggcatgc ggggcaatcc
     2521 gagtggcgcg gccgcgcacg gtgcaatgat ttttccatcg gagtggaact ggaaggcacc
     2581 gatgagcggg atttcgacga gtcgcagtac gccactctgg ccaggctcac cttagtcctt
     2641 ctgaattcct accctattaa ggaatgtacc ggccactcgg acatcgccac cccacgcggg
     2701 cgcaaaagcg accccggacc gcatttcgaa tggccgcgtt tccaggcgat gcttcgcgag
     2761 cgcggatagc gccttgcagc cgactggcgg agcgctgtca ccttatcggt accttgcggc
     2821 aacgcgactg aaaaacaact attgaccgcg cccaggcgta gttcatgggt acgattggta
     2881 ttgcttcacg cacccgcgtc cattgttaga tggcgaggct cggcgccatg cttccatggt
     2941 tgccgggatg gcaaagccca gttgtcggtc cgaactcttc tctgagtccg caaccctgcc
     3001 acagacttca acccaataac aatgccggac cgcaagtgga aattttgcgg ttgcatcatc
     3061 ggtttttatt tgatgcaagc gcaatcactt cgaattccgg gacatgaaaa ttttcttgac
     3121 agggttagtg aggctactag aattagtgaa aacataagcc atgggcacaa catatagtgg
     3181 tcggcgtcca ttttgaatcc tgctcggcgc gttgcttgca agcgacaaaa atacctgggg
     3241 gaaaattatg caattaggta cgaatctgac cgcaccggcg tccgtcggca attattcagc
     3301 agaaaacgcc ggaagcgatg agaaggagcg ttacgcgcaa tacaaagtca ttcgacgcaa
     3361 cggggcggtg gtttcgttcg agccgggcaa aattgccgtc gccgtaacga aggccttttt
     3421 ggctatcaat ggcggtcagg gcgcggcgtc ggctcgtgta cgcgaattgg tcgaaaaact
     3481 gaccgaagcg gtcgtaaacg cgctcatgcg ccggcagccg aacggtggca cgttccatat
     3541 cgaggatatt caggaccagg tcgaactttc gttgatgcgt tccggtgagc acgaagtggc
     3601 gcgagcctac gttctttacc gggaggaccg ggctcgcgag cgcgcccgaa ttcgggaggc
     3661 gcgtccggcg agcgccacgc ccggcattaa cgtggtcgaa gcgggccaaa cacggcccct
     3721 cgaaatggaa cggctgaccg aactcatcaa ggaatcctgt gaaggattag gccgggcggt
     3781 cgacgccggc ttgatcgttc agatgacgct gaaggatttg tacgatggcg taccgatgga
     3841 cgaagtgcgc aaatgtacga ttcttgccgc ccgatcgttg atagagaagg atcccgggta
     3901 tagctacgtg accgcgcgcc tgctacttaa tagtgtgcgg ttcgaagttc tgggtgaaga
     3961 ggtcgcccag actgagatgc attcgcgcta tgccgagtat tttccgcaat tcatcaggca
     4021 cggcatagcc gccggcttgc tggatgagcg attgggccgg ttcgacctga agtcgttggg
     4081 cgccgcgctg gatgccaatc gcgaccagaa attcggctac ctcggcctgc agactttata
     4141 tgaccgttac tttctgcata taagcgaccg gcgcatcgaa ttgccgcagg catttttcat
     4201 gcgtgtcgcg atggggctgg cgatcaacga ggttgaacgg gaagcacgtg cgatcgagtt
     4261 ctacaacgtg ctgtcgagct ttgatttcat gtcctcgacg ccgacattgt tcaattcagg
     4321 cactcagcgc tcgcaactgt ccagttgcta cctcacaacg gtcgcggatg atctggaagg
     4381 catctacgag gccatcaagg aaaacgcgct cttgtcgaag ttcgccggtg gactgggtaa
     4441 cgactggacc ccggtgcgcg cgctcggttc ccacattaaa ggaacgaacg gtaaatcgca
     4501 gggagtcgtc ccttttctca aagtagtgaa cgacaccgcc gtggcggtca atcagggtgg
     4561 caagcgcaaa ggcgcggtgt gctcgtacct ggaaacctgg cacctggaca tcgaggagtt
     4621 tctggaactg cggaagaata ccggagatga ccgccggcgc acccacgata tgaacacggc
     4681 gaactggatt ccagacctgt tcatgaaacg cgttatggag gctggagaat ggactttgtt
     4741 ctcgccagca gacgccccgg acctgcatga gaaggttggc aaggaattcg aggccgccta
     4801 ttgcgaatac gaacgccgcg cccgaacggg agaactgaag ctgttcaagc gcgtcccggc
     4861 gcttaccata tggcgcaaga tgttgacgat gttgtttgag accgggcatc catggatcac
     4921 cttcaaagat ccctgcaata tccgcagccc gcaacagcac gtcggcatgg tgcacagttc
     4981 gaatctgtgc actgaaatca cgctgaacac ttccgatacc gaaatagcgg tctgcaacct
     5041 gggttcggtg aacctccttg cccacatgaa ggacggcaag cttgaccagg agaagctgaa
     5101 agatacggta tcgactgcca tgcgcatgct ggacaatgta atcgacatca actattacgc
     5161 tgtcaacaag gcgcgcaacg ccaatttcaa gcaccgtccc gtcgggctgg ggatcatggg
     5221 atttcaggat tgcttgcacg aaatgcgcgt gccttacggg tcaagagaag cgatggagtt
     5281 cgccgatcgg tcgatggagg ccgtggccta ccatgcttac tgggcatcga cggagctggc
     5341 cgatgagcgt ggacgctact ccacttatga cggctcgtta tggtcgaagg gaattttgcc
     5401 gcaggattcg ctccagcttc tgaccgagca gcgcggcggc tatgtcgatg tggacaagtc
     5461 ctccggcctg aactgggagc ctctgcgagc ccggattcgc cagttcggta tgcgtaattc
     5521 caactgtttg gcgattgcgc ccaccgcgac catcgccaat atcgtcggcg tttccgcttc
     5581 aatcgagccg acctatcaga atctgtacgt caaatcgaac ctttccggcg aatttacggt
     5641 gaccaatgaa tatctggttc acgacctgaa gaaattgagc atgtgggacg aagtcatgat
     5701 cgcggacctg aagtatttcg atggcagcct tgccaagatc gatcgcattc cacaggaggt
     5761 tcgcagcctg tatgccactg cttttgaggt ggatccagtt tgggtggtgg aagcagggtc
     5821 gcgacggcag aaatggatcg atcaggctca gtcgctcaat atctacatgg ccggcgcttc
     5881 aggaaaaaaa ctggatgaaa cgtacaagct ggcctggacc agcgggttga aaacgacgta
     5941 ttacttgcgc accctcggcg cgacatctac ggaaaaatcc acggtgcgag gaggtcagtt
     6001 gaacgcagtc ccgactgacg gcgggttgtc cggagcagga acgactcagg cggcgatcga
     6061 agatgccaag ttctgcgcca ttgacaatcc agaatgcgag gcttgccagt gaagggtaaa
     6121 gccggcgtta ttgattgcgg cggaggctaa cttggacttt gccaagttaa gcgcgttgca
     6181 caacaggaac gataagcccg gaatgcgaag catgccagtg gctgagcacc atggccttta
     6241 tgtttcctgc attttgatgg attgcgcgcg tcatgacatc cggttgtctg cgccatgtcg
     6301 gctgttggcg ctgaaccgag aatgcagtcc cagacgcgaa gtctgccaat aatggtcaga
     6361 aagaaacggc aagttgccaa taatg
//
LOCUS       DATMZQ010000234         4822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2943694, whole genome shotgun sequence.
ACCESSION   DATMZQ010000234 DATMZQ010000000
VERSION     DATMZQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943694"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VN496_07670"
     CDS             <1..292
                     /locus_tag="VN496_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16867.1"
                     /translation="MVRENDNTVYSVQIDRIESFKTREGVHYGSSEALVRAGLGRPQS
                     IQESAISEMPRAYCYISGLGVQLDATGHVASLIVFPGSDLRKICKAQPAKTP"
     gene            complement(307..1008)
                     /locus_tag="VN496_07675"
     CDS             complement(307..1008)
                     /locus_tag="VN496_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HXF16868.1"
                     /translation="MIAARYALYLVPHRDSLLWETACRWLGRDPETGLQLSQPEVPGY
                     DAQRVAQITHSPRHYGFHATLKAPFRLAEGADESQLMTRVAQLAHSLPAFVMPPLQVD
                     TISGFLALRPAGAAVQLEAMAETCVTTLDALRAPLDASERKRRHAAGLTARQRDLLEH
                     WGYPFVLDEYRFHMTLTQRLDDRDAGFLLPWLQGWFAPAITSATNQAELAVFVQPQLE
                     AEFRLLRRFALMGRG"
     gene            1079..2215
                     /locus_tag="VN496_07680"
     CDS             1079..2215
                     /locus_tag="VN496_07680"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HXF16869.1"
                     /translation="MQRLITNARIVTNSDSFIGTVAIENGLIGEYDSGPTSLPGAENW
                     EGDYLIAGLVELHTDNLEKHLEPRPGVRWPAMSALLTHDAQIASAGITTVLDAMGVGD
                     FDARSIRASGLHAATEALNHAREQGLLRAAHLLHMRCEIACDNVVEVVEPFLDDPTVR
                     LVSLMDHTPGQRQWMDVEKFRTYTQRNQKWSDEYLAQVVKERMDMQVRNSGQNRLQLL
                     ELCRDRSLPMASHDDTVPEHVEEAVAAGFRISEFPTTKSAASAARKHGLGIVMGAPNL
                     VRGESHSGNISALELAKADLLDVLSSDYIPYSLLHAVFLLRDQAGWALPKAVATATLN
                     PAHLVGLDDRGEIAPGKRADYVRVRESRGVPVPLETWRDGKRIA"
     gene            2232..3128
                     /locus_tag="VN496_07685"
     CDS             2232..3128
                     /locus_tag="VN496_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF16870.1"
                     /translation="MKIAIPGKHMMQYLTAPARIWLLALVAMMVMPLQSLAQTLSIGL
                     IPSEDPRLMASEYQPLIDHLAKALRMEVKPFVATDYNGVIEALRSRKLDVAMLGPFSY
                     VLATTVADVEAFAAPETSKAGLSYHAVIIARKDRGFRTLLDLKGRNFAFVDPSSTSGH
                     LFPKAALVKLGYDPDDFFGRVVFSGGHDASVLSVQNGKIDAAAVADTLLEAAYSRGIV
                     KREDLQILWTSEPIPTVPYVMRKDLPDELRKRIRAAFLQIRDLPVGSHATIVRVDPID
                     DSAYDGIRETAKVLKLELKKFK"
     gene            complement(3158..3610)
                     /locus_tag="VN496_07690"
     CDS             complement(3158..3610)
                     /locus_tag="VN496_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HXF16871.1"
                     /translation="MAEPKSLYSEVILDHIKNARNFHELRDASHRAHGINPLCGDTFD
                     VFAKVEGGRVVEAAFQCECCGISMASASIMTETVQGKTVQEARTLYKSFEKLLRDPAA
                     ASELHADAGQLPMLELIREFPSRVNCALLGWHTLDAALDGRPDVTISA"
     gene            complement(3625..4377)
                     /locus_tag="VN496_07695"
     CDS             complement(3625..4377)
                     /locus_tag="VN496_07695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF16872.1"
                     /translation="MTSRSLQDHVAVIVGGSGAIGSAICRHFAEAGAICVVTYNNGER
                     AARDLVATLPGDGHSAAHIAVDDSDALRALATDVENTHGRTDVLVNCAGITRFVQHSD
                     LDALDDRLIDDIFRVNWRGPFAAVRAFRTLLAARGDGLVINISSIAGVTAMGSNVAYC
                     ASKAALDSMTKSLARALAPAIRVVSVSPGLVDTDFVKNLDQKWRDEQAARTPLKRLAT
                     ADEIGAAALAVVLHLQYSTGCIIPIDGGRPLA"
BASE COUNT         1072 a         1427 c         1370 g          953 t
ORIGIN      
        1 gatggtgagg gaaaacgata atacggttta ctcggttcag atcgaccgga tcgaaagttt
       61 taaaacccgc gaaggcgtgc actacggctc ttccgaagcg ctggtgcgcg ccggtctcgg
      121 cagaccgcaa tccatccagg agtccgctat cagtgaaatg ccccgcgcgt attgctatat
      181 cagcggactt ggcgtgcaac tggatgccac tggacatgtt gccagcctga tcgtgtttcc
      241 aggttcggac ttgcgcaaaa tctgcaaggc gcaaccggcc aagacaccct gatctctgct
      301 gtcaaatcaa ccgcgaccca tcagcgcgaa acgtcgcagc aaccgaaact ctgcttcgag
      361 ttgcggttgc acgaaaacag ccagttctgc ctggttagta gcggaagtta tcgctggcgc
      421 gaaccagccc tgcagccagg gaagcaggaa gccggcatcg cgatcgtcga gccgttgcgt
      481 tagcgtcatg tgaaagcgat actcgtccag cacaaacggg tatccccagt gctcaagcaa
      541 atccctttga cgcgcggtta atccggcggc gtgcctgcgt ttacgctcgc ttgcgtcaag
      601 cggcgcgcgc agcgcatcga gcgtggtcac gcaggtttct gccattgcct ccagttgcac
      661 cgctgcaccg gcggggcgca aggcaaggaa gccgcttatc gtgtcgacct gcagcggtgg
      721 cattacgaaa gcaggcagcg aatgcgcaag ttgcgcgact cgtgtcatca attgcgattc
      781 gtccgcacct tcggccagtc tgaacggggc cttgagggtc gcatggaaac cgtagtgcct
      841 cggtgaatgc gttatttgtg ctacgcgctg cgcatcatat cccggtacct ctggctggct
      901 aagctgcaat cctgtttcgg ggtcgcgacc cagccagcga caggcagttt cccacagcag
      961 ggaatcccga tgcggaacga ggtacagcgc atatcgggca gcaatcacga tgagtttgaa
     1021 gacatgggct gcagtatcat acggccttcg gaatgtgcat gcccccagag tcttccttat
     1081 gcaacgtctc attaccaatg cgcgcatcgt cacaaacagc gactcattca tcggcaccgt
     1141 tgccatcgaa aacggcttga tcggagagta cgacagcggg cccacttccc tgccgggcgc
     1201 agaaaattgg gaaggcgatt atctgattgc cggcctcgtc gaactccata cagacaatct
     1261 ggagaaacac ctggaacctc ggcccggcgt gcgctggccg gcaatgtctg cgctgctgac
     1321 gcatgatgcg cagatcgcca gtgcgggcat cacgacggta ctcgatgcca tgggcgtcgg
     1381 cgatttcgac gcgcgttcga ttcgcgccag cggcctgcac gctgccactg aagcactcaa
     1441 ccacgcacgc gaacagggat tgctgcgcgc agcgcacctt ctgcacatgc gctgcgaaat
     1501 tgcatgtgac aatgtggtgg aagtagtcga gccgttcctc gatgatccga ccgtaaggct
     1561 ggtgtcattg atggaccata ctccaggaca gcggcagtgg atggacgtcg agaaattccg
     1621 tacctacacc cagcgcaacc agaaatggag cgacgaatat cttgcgcaag tagtaaaaga
     1681 gcgcatggat atgcaggtcc gtaactccgg gcaaaatcgc ctgcagttgc ttgagctttg
     1741 ccgcgatcgc agcctgccca tggcgagcca tgacgatacc gtgccagagc acgtcgagga
     1801 agcggtggca gccggattcc ggatttcaga atttccgacg acgaaaagcg ctgccagcgc
     1861 agcgcgcaag catggattgg gcatcgttat gggggcgcca aaccttgtgc gtggagaatc
     1921 acactcggga aacatttcgg cgctggagct ggccaaggct gacctgctcg acgtgctttc
     1981 gtcggattat attccctata gcctgttgca cgcggttttc ctgctgcgcg atcaggctgg
     2041 atgggcgctg cccaaagccg tggcaaccgc cacactgaat ccagcccatc tggtcgggct
     2101 cgatgaccgc ggagaaatcg cgccggggaa gcgggccgat tacgttcgcg tgcgcgaatc
     2161 gcgcggtgtg ccggtgccgc tcgagacgtg gcgcgacggc aaacgaatcg cgtaaatccc
     2221 cgtaatactc aatgaaaatc gcaataccgg gaaaacacat gatgcaatat ctgactgcac
     2281 cggcgcgcat ctggctgctc gcgctagtag caatgatggt gatgccgctg cagagccttg
     2341 cccaaacgct aagcatcggt ctcatccctt ccgaagaccc gcgcctcatg gcgagcgagt
     2401 accagccgct aatcgaccac ctcgccaaag cgctccgcat ggaagtcaaa ccatttgtcg
     2461 caaccgatta caacggcgtc attgaagcgc tacgctcaag aaagctggat gtagcgatgc
     2521 tgggaccctt ctcttacgtg ctggcaacga ccgttgccga cgtcgaggca ttcgcggcgc
     2581 cggaaaccag caaagctggc ctttcctatc acgccgttat cattgctcgt aaggaccgcg
     2641 gcttccggac actcctggat ctgaagggac gcaatttcgc attcgtcgat ccaagctcaa
     2701 cctccggaca tctgtttccg aaagccgcgc tggtaaaact ggggtatgac ccggacgact
     2761 ttttcggccg cgtcgttttt tccggtgggc acgatgccag cgtgctcagc gtacagaatg
     2821 gcaagataga cgcggcagcg gtggcagaca ctttgttgga agccgcatac tcgcggggga
     2881 tcgtcaaacg cgaagatcta cagatcctgt ggacttcgga acccattccg acagtgcctt
     2941 acgtgatgcg caaagatctt cccgatgagt tgcgcaaacg catacgggcg gcctttcttc
     3001 agattcgcga cctgccggtc ggcagccatg ccaccatcgt acgcgtcgat ccgattgacg
     3061 attccgctta tgacggcata cgggaaacgg ccaaagttct caagctggaa ctcaagaaat
     3121 tcaagtaaaa aatccgcaga agtgagcgca cgaccgatca agcggaaatg gtgacgtccg
     3181 gtcggccatc gagcgcggca tccagcgtgt gccagcccag cagcgcgcaa ttgacccgcg
     3241 acggaaattc ccgaatcagc tcgagcatcg gcaactgacc ggcgtccgca tgcaattcgg
     3301 aagcggccgc aggatcccgc aacaacttct cgaaactttt gtaaagcgtc ctggcctcct
     3361 gcacagtctt gccctgtacg gtttcggtca tgatcgaggc cgatgccatc gatatcccgc
     3421 agcactcgca ctgaaaggcg gcctctacga cccggcctcc ctcgactttc gcaaacacgt
     3481 caaaggtatc gccgcagagg ggattgatgc cgtgcgctcg atggctggca tcccgcagtt
     3541 catggaaatt gcgcgcgttc ttgatgtgat ccaggatcac ttcactgtag aggcttttcg
     3601 gttctgccac ggacgaagat tccttcaagc gagcggcctt ccgccgtcga tcgggataat
     3661 acagcccgtg gaatattgta aatgcagcac caccgcgagc gcggcggcgc caatttcatc
     3721 ggcagtcgca aggcgtttca gcggtgtacg cgcggcctgt tcgtcacgcc acttttggtc
     3781 caggttcttc acaaaatccg tatcgacgag acctggcgaa accgagacca ctcgaatggc
     3841 tggcgctaac gcccgcgcca aagatttggt catggaatcc aatgccgcct ttgacgcgca
     3901 ataggccacg ttactgccca tcgcggttac ccctgcaatg gaggaaatgt tgatgaccag
     3961 cccatcccca cgcgccgcaa gtaatgttcg gaacgctcgc accgcggcaa atggcccgcg
     4021 ccagtttacg cggaagatat cgtcgatcaa ccggtcatcc aacgcatcga gatcgctgtg
     4081 ctggacgaat cgggtaatgc ccgcgcaatt caccagcaca tccgtacgac catgcgtgtt
     4141 ctcgacgtcc gtcgccagcg cacgcaatgc atcgctgtca tccactgcaa tatgcgcggc
     4201 agaatgtcca tcgcccggca gagtcgcgac cagatctcgg gcggcgcgtt ctccgttgtt
     4261 atatgtaacg acacaaatcg cgcccgcttc agcgaaatgg cggcaaattg cggaaccaat
     4321 cgctccagag ccgccaacaa taacggcaac atggtcttgc aagctgcgtg aagtcatgcg
     4381 ggtattgaac cacagaaggt gccattactg ggacggcacc cgacgactct gcgttaccgt
     4441 gcccgacgtg agggtagttt cgccacgact gaccgattgc tatgcgaccc catgggcgtt
     4501 gcagggccct gagcaaacgt cttttcaggg acaacggaac cctcgtctgt ctgccgttga
     4561 agcatatccc gaaaaagtcg ttgcgtggtg tctgatggcg gaactccgag cacaactgaa
     4621 agcgtatgct tgagacggcg gtaactactg aaggcctcgg tacgcttacc gagccgctcg
     4681 taacagcgca tcaatccttg gtggaatgat tcgacaattg aatcagcttc gatgcctcgc
     4741 aggtaacatt gcaaggcgcc tgcgagatcg ccctgcgatt ccagtaccgc ggcgtgtccg
     4801 gaaagcgcgt gaataaattt gc
//
LOCUS       DATMZQ010000235         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3980496, whole genome shotgun sequence.
ACCESSION   DATMZQ010000235 DATMZQ010000000
VERSION     DATMZQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3980496"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="VN496_07700"
     CDS             <1..1901
                     /locus_tag="VN496_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXF16873.1"
                     /translation="LFQAVVGIAVSMIPEGLPALITITLAIGVQRMAQRSAIIRRLPA
                     VETLGSVSRICSDKTGTLTLMEMMVVSAVTAESTYQITGNGYAPEGEVKKDGQPIKKD
                     AVLGLMGQVSTLCNDAELFQHEGTWKVEGDPTEGALYPFAAKLGMDREKERTASPRID
                     AIPFESEHKFMATLHKSAAGKEILLVKGAPEVILEHCDRQQVADGQPPSLDRKHFMEA
                     SDKLAAQGERVLALAWLENPAVKKGLSPADLPKTLVLIGLIGLLDPPRKEAIEAVKEC
                     HTGGIRVTMITGDHKITAAAIAKMLGIGDGKTAITGTEIEEMSAATLQEKVKDVDVFA
                     RASPEHKLRLVKAIQANKQIVAMTGDGVNDAPALKKADIGVAMGIKGTEVTKEAAGMI
                     LADDNFASITAAVKEGRTVYNNIEKAILFMLPTNVAQALVIAVAIFVGFTMPITAPQV
                     LWVNMVTSVALGLVISFEPHELDVMNRPPRSVDRPILTGFGIWRVIFVGLALLAFTLW
                     AFFWMKSLNASDALARTVAVNAITIGQVFYLLNSRYLLDSSLSLKAHLGNKYLPLGIG
                     AVVILQLLFTYAPPLQRLFDNEAVPLWVWPWLLAGGLVFFIVVEIEKLIIRSTGLRSA
                     VTAVEAGA"
     gene            complement(1912..>2258)
                     /locus_tag="VN496_07705"
     CDS             complement(1912..>2258)
                     /locus_tag="VN496_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16874.1"
                     /translation="AQLCAQAMQRASSIAEKVAVLPEAHPVTIRSGLLPTSPEAPANS
                     GAQESIAELERCANALSELRRSHRSTTLSEVAKGALTADQAIVRVDTVRNLEALTNHA
                     WRSAAHLMGLGT"
BASE COUNT          463 a          645 c          688 g          462 t
ORIGIN      
        1 agctctttca ggccgtggtc ggtattgcgg tttcgatgat cccggaaggc ctgcctgcac
       61 ttatcacgat aacattggca atcggcgtgc aacgaatggc gcaacgcagc gcgatcattc
      121 gcaggctgcc ggcggttgaa acactcggct cggtatcgcg tatctgctcc gacaagaccg
      181 gtaccctgac gctcatggag atgatggtcg tttccgctgt aacggcggaa tcgacctatc
      241 agataaccgg caacggttac gccccggagg gcgaggtcaa gaaggacgga cagccaatca
      301 aaaaggatgc agtcctcggg cttatgggtc aggtctccac gctgtgtaat gacgcggagc
      361 tcttccaaca cgaaggaacg tggaaggtag agggagatcc gacggaaggc gcgctctatc
      421 ccttcgctgc caagctcggc atggatcgag agaaagagcg gacagcatct ccgcgtatcg
      481 atgcgattcc attcgagtca gaacacaaat tcatggccac gctgcacaag tctgccgcgg
      541 gcaaggaaat tcttctggtc aagggcgcgc ccgaggtgat ccttgaacac tgcgatcgtc
      601 agcaggtcgc ggatgggcaa cctccgtcgc tcgaccgcaa gcacttcatg gaagcttcgg
      661 acaagctggc cgcgcagggc gagcgcgtat tggccctggc gtggctggaa aatccggctg
      721 taaaaaaagg gctcagtcct gcggacctgc cgaagactct ggtcctgatt gggctgatcg
      781 gattgctgga cccgcctcgc aaggaggcca tcgaagcagt caaggaatgc cataccggcg
      841 gcattcgcgt cacgatgatc acgggcgatc acaagatcac ggcggcagcc atcgccaaga
      901 tgctcggcat cggggatggc aaaaccgcta tcaccgggac cgagatcgaa gagatgagcg
      961 ctgcgacgct gcaggagaaa gtcaaagacg tcgacgtatt cgcgcgcgca agcccggaac
     1021 acaagctccg gctggtgaaa gccattcagg ccaacaagca aatcgtggcg atgacgggcg
     1081 acggcgtaaa cgacgcaccg gcgttgaaaa aagccgacat cggcgtcgcc atgggcatca
     1141 aaggtaccga ggtgacgaag gaagccgccg ggatgatcct cgcggacgat aacttcgcct
     1201 cgatcactgc cgcggtaaag gaaggccgta ctgtctacaa caacatcgaa aaagcgatcc
     1261 tgttcatgtt gccgaccaac gttgcccagg cgctggttat cgcggttgcg atcttcgtcg
     1321 gcttcaccat gcccatcacg gcaccgcagg ttttgtgggt caatatggtg acctccgtcg
     1381 cgcttgggct cgtcatttcg tttgagccgc atgagctgga cgtcatgaac cggccgccgc
     1441 gcagcgtgga tcggccaatc cttaccggct tcggaatttg gcgcgtcatc tttgtgggac
     1501 tggcgctcct cgcatttacg ctctgggcgt tcttctggat gaaatcgctg aatgcctcag
     1561 atgcgctcgc ccgcaccgtt gccgtcaacg ccattacgat cggccaggtg ttttaccttt
     1621 tgaacagccg ttacttgctc gactcttcgc tttctctgaa agctcatctt ggcaacaagt
     1681 atctgccatt gggaatcgga gcggttgtta tcctgcagct tttgtttacc tatgcgccgc
     1741 cgttgcagcg tctttttgac aacgaggcgg tcccgctgtg ggtctggcca tggttgctgg
     1801 caggcgggtt ggtgtttttt attgtcgtgg agatcgaaaa gctcattatt cgttcaaccg
     1861 gtctgcgcag cgcggtgacg gccgtggagg caggtgcata gctgtgcgta gctacgtgcc
     1921 gagccccatc agatgcgctg cagaccgcca ggcgtggttc gtcagagcct ccagattgcg
     1981 tacggtatcg acgcgaacga ttgcctgatc cgctgtcagt gcgcccttcg ccacctcact
     2041 cagcgtcgtg ctccggtgcg agcgacgcag ctcgctcagt gcattggcgc agcgctccag
     2101 ttcggcgatg gactcttgcg cccctgaatt ggccggggcc tcggggctgg ttggcagcaa
     2161 gccgctgcga attgtaacag ggtgggcctc cggcaaaacc gcgaccttct cagcaatcga
     2221 tgatgcgcgc tgcattgctt gcgcgcacaa ctgcgcgg
//
LOCUS       DATMZQ010000236         5698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1218825, whole genome shotgun sequence.
ACCESSION   DATMZQ010000236 DATMZQ010000000
VERSION     DATMZQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218825"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..3363)
                     /locus_tag="VN496_07710"
     CDS             complement(157..3363)
                     /locus_tag="VN496_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXF16875.1"
                     /translation="MSQKMVRAGLAYVLLIAVLFASGATPRVPASEQAPLRLLDHFDD
                     LGGWRVSTSDDVKAKLRAGAGQNGNALCADFDFGTVSGYVTVGRELALDYPDNYEFSF
                     WIRGDAPANALQFKLVDNTGENVWWVNRPDYVFAHEWRRVSFKKRNISFAWGPSKDHE
                     LSHSAQLEFVIARGREGGKGSVCFDELALRTLPPASAAVPVATANASSMLAPTRPAQA
                     FDGNSSTAWRSDPAMGAEQTLTLDFQQPREFGGLVLHWLPGEHASRYTIELSDDGERW
                     QTVRHVIDGNGATDPHFLPESEARYVRLHLEDGPGHAYGLAEIEIKDSAYGDSQNGFI
                     ESIAKDASFGEYPRAFTGQQSYWTILGVDGGTAQGLLSEDGAIEISPRSGSIEPFLLE
                     GENFISWADVRAQQSLREGYLPIPTVTWLRGSLIMRTTVFASGNRDRSQLLARYTIEN
                     TSDRPRAITLALAIRPFQVNPPTQFLNVPGGVSPIHALAWDGQAVTINGQRTVWPLQT
                     PDQFIAADFDAGTIAQIIARPAAHPANAVKDETGLASGVLLYRLELPAAGSRSVGLVA
                     PLTGTPVLPAKDPVSWMDLEQERTAAYWSSRLNLVSLHMPVQAGPLPDVLRTALAHLL
                     INRTGPALQPGSRSYARSWIRDGSMMGDALLRLGHAPVVREFAQWFASHQFSNGKVPC
                     CVDNRGADPVAENDSQGELIHLIAQQYRYTRDRAWLQEMWPHVASAVSYMNLLSSTQR
                     TEQNLSPGRRAFYGLMPASISHEGYSDRPAYSYWDDFWSLAGYADAFDMARTLGRKDA
                     AEIRARLEQFRSDLHASLVASIAQHGIDYLPASADRGDFDPTATTIALSVAGAQAQLP
                     QPQLQHTFERYWNDFVQRRDGDTQWMEYTPYELRQVGALVRLGWRERATQLLNFFLAD
                     RRPIGWNQWAEVVGRDARQPRFVGDMPHTWIASDFIQSALDLFAYERPEDHALVLGAG
                     VPADWLAGPGIGIEKLRTPYGSLSYALHRDGQHLLLSIDEGLNPPSGGLIYQWPYATA
                     PGRVLSNGQAIQWSNNRELRIQTLPAEIDIELSGNMKQ"
     gene            complement(3366..4187)
                     /locus_tag="VN496_07715"
     CDS             complement(3366..4187)
                     /locus_tag="VN496_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXF16876.1"
                     /translation="MKTPVASILVNGMLAGLTLVSLFPLLWMLSVSFMTPGEASTLPP
                     PLLPESPTLVNYRELFMRGGMTRYLLNSVFVATVITVISLLFNAMAGYAFAKLQFAGQ
                     QRLFKALIGTLIIPGQVAMMPLFLLLKQMGLVNSYGGLIAPAMASIFGIFLVLQYALS
                     IPDELMEAARIDGAGEFRIFFSIIMPLLAPILASLALFTFLGSWNDFMWPLIVLTDAH
                     LQTLPVALASLSREHAQDGELMMAGSVVTILPVLLLFLALQKYYIRGLLVGSVKG"
     gene            complement(4184..5062)
                     /locus_tag="VN496_07720"
     CDS             complement(4184..5062)
                     /locus_tag="VN496_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXF16877.1"
                     /translation="MRLPPLAWAFVAPALLVIGFFFAFPVVAGLALSFTDFDIYALAD
                     TGNLRFVGLDNYLMLLRTPLFWQALGNTLYFVVIGVPLSIGLSLGSALLLHARVARFK
                     PFFRTALFAPVVTTLVAVAVVWRYLFQTRYGMVNYVLAHLGIAPLDWLGDPHLAMPTI
                     ILLAVWKNFGYNMVIFLANLQAVPEEVYEAARIDGASLFQQLVYITLPILRPTLILVG
                     ILTTAGYFQLFAEPYVMTQGGPLRSTVSVLYFMYEEGFKWWNFGTASTVAVLLFILMF
                     AVTWTLMRVSRSEAAA"
     gene            complement(5059..>5698)
                     /locus_tag="VN496_07725"
     CDS             complement(5059..>5698)
                     /locus_tag="VN496_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXF16878.1"
                     /translation="WGHFSGPGFHRALDFYAGMFRNGWAPAMNNSAISNVWTEFGRGY
                     FSFYMSGPWNIEEFKRRLPAEHRGDWLTAPLPGPDGPGASTAGGSSLVIFRSTRRTQA
                     AWRMIEYLSEPATQVRFHALTGNLPPRRTPWQDEHLANDIYAKAFRDQLERARPAPKV
                     PEWERIMNEMQLAAEQVVTGGVSIDEATRSLDRRVDAILEKRRWMLAHGKPQ"
BASE COUNT         1246 a         1687 c         1693 g         1072 t
ORIGIN      
        1 attggtacga taaatcaaac atctccgatc cgaagattgc ggcggttctt tacgattgat
       61 gagtttggta aatggcgcgc gtcgggctcg ctggcggttg gcccgatgcg taccagcggt
      121 cgcatacggc gcggggaatg tttttcatgc ggccgcttac tgcttcatat tcccggaaag
      181 ctcgatgtct atctcggccg gcagcgtctg gatgcgcaac tcacggttgt tgctccactg
      241 tattgcctga ccgttgctga ggacgcggcc cggcgcggtc gcatagggcc attggtagat
      301 taatccgccc gacggaggat tcagtccttc gtcgatactc agcaatagat gctggccatc
      361 gcgatgcagc gcataactca agctgccgta aggcgtgcgc aatttctcta tgccgatgcc
      421 tggcccggcc agccaatcgg ccggaacgcc ggctcccagc accaacgcgt gatcctcggg
      481 tcgctcgtag gcgaataagt cgagcgcgga ttgaatgaaa tccgatgcaa tccaggtgtg
      541 gggcatatcg cctacaaaac gtggctggcg tgcgtcgcgg ccgaccactt cggcccactg
      601 gttccagccg atgggtcgac gatcggcgag gaagaaattt agcagttgcg tcgcgcgctc
      661 gcgccagccc aggcgcacaa gggcgccaac ctggcgcaat tcgtagggcg tgtattccat
      721 ccactgggtg tcgccatcgc gccgctgcac gaagtcgttc cagtatcgct cgaacgtgtg
      781 ctgcaattgc ggctggggca actgggcttg agcgccggcc acggacagcg caatcgtggt
      841 tgccgtggga tcgaaatcgc cgcggtcggc gctagcgggg aggtaatcaa tgccgtgttg
      901 cgcgatgctt gcaaccagcg atgcatgcag gtcgctgcgg aactgttcga ggcgggcgcg
      961 gatctccgcg gcgtctttgc ggccaagcgt tcgcgccatg tcgaatgcat cagcgtagcc
     1021 cgcgaggctc cagaagtcgt cccagtacga ataggccggc cggtccgaat agccttcgtg
     1081 actgatcgaa gccggcatca atccgtagaa tgcgcgccgt cccggactca ggttctgctc
     1141 tgtgcgttgt gtgctgctca gcaggttcat ataagacacg gcggaggcaa catgcggcca
     1201 catttcctgc aaccatgcgc ggtcacgcgt gtagcggtac tgctgagcga taaggtggat
     1261 cagttcaccc tgactgtcgt tttccgctac gggatcggcg cctcgattgt ccacacagca
     1321 gggcaccttg ccattcgaaa actggtggga cgcaaaccat tgcgcgaatt cgcgcacgac
     1381 cggcgcgtgg cccaggcgca gcaacgcgtc ccccatcatg ctgccatcgc gaatccacga
     1441 gcgcgcgtac gagcggctgc ccggctgtag agccggtcct gtccggttga tgagcaggtg
     1501 ggccagtgca gtgcgcagaa catcggggag tggtcctgcc tggacgggca tgtgcaggga
     1561 aaccaaattt aatctgctgc tccaatacgc cgcggtacgt tcctgctcga ggtccatcca
     1621 gctcaccgga tcctttgccg gcagcacggg cgtgcccgtc aggggagcca ccaagcccac
     1681 actccggctg ccggcggctg gcaattccag ccgatatagc agcacgccgg aagccaggcc
     1741 ggtctcgtct ttgacggcat tggccggatg tgcagcaggt ctcgcaataa tttgtgcaat
     1801 cgtccccgca tcgaaatccg cggcaatgaa ttggtctgga gtttgcagcg gccatactgt
     1861 gcgttgccca ttgatggtta ccgcctgtcc gtcccatgcc aacgcgtgaa tcggtgatac
     1921 gcctcccggc acattcagaa attgtgttgg aggattgacc tggaacggcc tgatcgccaa
     1981 tgccaacgtt atagcgcgcg gacggtcgga cgtgttttcg atggtgtacc gcgccagcag
     2041 ctgagagcga tcacgattgc cgctggcgaa caccgtagtg cgcatgatca gactgccgcg
     2101 caaccatgtt acggtcggaa tgggtagata gccctcgcgc agcgactgtt gtgcacgcac
     2161 atcagcccag gagataaagt tttcgccctc gagtagaaac ggttcgatgg agccggagcg
     2221 gggcgaaatt tcgatcgcgc catcttcgga cagaagtccc tgggccgtgc cgccgtcaac
     2281 gccgagaatg gtccagtagc tttgctgtcc cgtgaacgct cgcgggtact cgccaaaact
     2341 cgcgtccttg gcgatagact cgatgaaacc attctgcgaa tctccgtaag cggaatcctt
     2401 gatctcgatc tccgccaggc cgtacgcgtg gcccgggcca tcttccagat gcaatcgcac
     2461 gtagcgcgct tccgattcgg gcaagaaatg ggggtcggtg gcgccgttgc cgtcgatgac
     2521 gtggcgcacc gtctgccatc gctcgccgtc gtcggaaagt tcgatggtat agcgcgaagc
     2581 atgctcgccc ggtaaccagt gcaacaccaa gccaccaaat tcgcgtggct gctggaaatc
     2641 cagcgtcaat gtttgctccg cacccattgc tggatcgctg cgccaggccg tcgagctgtt
     2701 tccatcgaac gcttgagccg gccgggttgg tgcgagcatt gaactggcat ttgccgtcgc
     2761 aacgggcaca gcagcactcg cgggaggcag cgttcgcaaa gccagttcat cgaaacacac
     2821 actgcctttg ccgccttcgc gccctctcgc aatcacaaac tccagttgcg cgctgtgtga
     2881 gagttcatgg tctttggacg gaccccaggc gaatgagatg ttccgtttct tgaagctcac
     2941 gcgtcgccat tcatgagcga agacatagtc agggcgattg acccaccaga cattttctcc
     3001 agtgttgtcg accagcttga actgcagtgc atttgcgggc gcatcgccgc gtatccagaa
     3061 cgaaaattcg tagttgtccg gatagtccaa tgccagctcg cggccgaccg tcacatagcc
     3121 tgacaccgtg ccgaagtcaa aatccgcgca cagcgcattg ccgttttgtc ccgcccctgc
     3181 gcgaagtttt gctttgacgt cgtcggacgt ggagacgcgc cacccgccaa ggtcatcgaa
     3241 gtgatcaagc aagcgcagcg gggcctgttc agaagccggt acacgcgggg tagcgccaga
     3301 cgcgaacagg acagcgataa gcaaaacgta cgcaagcccc gcgcggacca tcttttgcga
     3361 cacgttcatc ccttgacgct accgaccagc aggccgcgga tgtagtattt ttgcaaggcg
     3421 agaaacagca acagcaccgg cagaatcgtc acaactgaac ccgccatcat cagctcgccg
     3481 tcttgcgcat gctcgcggga caaagatgcc agcgcgaccg gcaaggtctg caaatgcgcg
     3541 tcggtcagca cgatcagcgg ccacatgaag tcgttccagg aacccaggaa cgtaaacagg
     3601 gccagcgacg ccaggatcgg tgccagcagc ggcatgatga tgctgaaaaa gatccggaat
     3661 tctccggcgc catcgatgcg ggccgcttcc atcaactcgt ccgggatcga caacgcgtat
     3721 tgcagtacca gaaaaatgcc gaatatgctg gccatggccg gcgcaataag cccgccgtaa
     3781 ctgttcacca gccccatttg tttaagcaac aaaaacagcg gcatcattgc cacctggccg
     3841 ggaatgatca gggtcccgat cagcgccttg aacaggcgct gctggccggc gaactgcagc
     3901 ttcgcgaacg cataaccggc catggcgttg aacagcagcg aaatcacggt aatgactgtg
     3961 gcaacgaaca cactgttcag caggtagcgc gtcatgcctc cgcgcatgaa cagttcccga
     4021 taattgacca gtgtgggcga ctcgggaagc aatggcggcg gcagtgtgct ggcttcgccg
     4081 ggagtcatga aggataccga tagcatccac agcaacggaa acaggcttac cagcgtcaag
     4141 ccagcgagca tcccgttaac cagaatggaa gcaacaggtg ttttcacgcg gctgcctcgc
     4201 ttctggaaac gcgcatgagc gtccaggtca ctgcaaacat cagtatgaaa agcaataccg
     4261 caacggtgga agcagtgccg aagttccacc acttgaagcc ttcctcatac atgaaataca
     4321 acacactgac cgtgctgcgc agcggaccac cttgcgtcat gacgtagggt tccgcaaaca
     4381 gctggaaata tcccgctgtg gtcagtatcc cgaccaggat caatgtcggc cgcaagatcg
     4441 gcagcgtgat gtaaaccagc tgctgaaaca acgatgcacc atcgatgcgc gccgcttcgt
     4501 aaacttcttc cggaacagcc tgcagattgg ccagaaagat caccatgttg tagccaaaat
     4561 ttttccagac ggcgagcaaa atgatggtgg gcatcgccag gtgcggatcg ccgagccagt
     4621 cgagcggagc aatgccgagg tgagcgagca cataattcac catgccgtaa cgcgtctgaa
     4681 aaagataacg ccataccacg gcgacagcga ccagtgtggt gacgaccggg gcgaacagcg
     4741 cggtgcgaaa aaagggcttg aagcgcgcga cccgcgcgtg caacaacagc gccgagccga
     4801 gcgacaagcc aatcgaaagc ggcacgccaa tgacaacaaa atatagagta ttgcccaatg
     4861 cctgccagaa aagcggagtt cgcaacagca tcagataatt gtcgagaccg acgaagcgca
     4921 ggttgcctgt gtcggcgagc gcgtagatat cgaagtccgt gaagctcagc gccaagccgg
     4981 caacgacagg gaatgcgaaa aagaagccga tcaccagcaa ggccggggca acgaatgccc
     5041 atgccagcgg cggcagcctc attgcggttt gccatgcgcc aacatccagc ggcgcttttc
     5101 gagaatggcg tcgacgcgcc ggtcgagcga acgcgtagcc tcgtcaatgc tgacgcctcc
     5161 agtgacgact tgctccgccg caagctgcat ttcattcatg atgcgctccc actctggaac
     5221 ttttggcgcg gggcgcgcgc gctcgagttg gtcccgaaac gcttttgcat aaatgtcatt
     5281 tgcaagatgc tcgtcctgcc atggagtgcg cctgggcggc agatttccgg tcagcgcatg
     5341 gaagcgaact tgcgtggcgg gttcggaaag atattcgatc attcgccatg cggcctgcgt
     5401 gcgccgggta gagcggaaga tcaccaggct ggagcctcct gcggtggagg cgcctggacc
     5461 gtcgggtcca ggcagcggtg cagtcaacca gtcgccacga tgctcggcag gcaagcgacg
     5521 cttgaactcc tcgatattcc atggcccgga catgtagaag gaaaaataac ctcgtccgaa
     5581 ctcggtccag acgttggaaa tggcgctgtt gttcatggcc ggcgcccagc cattgcgaaa
     5641 catccccgca taaaaatcca gtgcgcggtg aaatcccggc ccggaaaaat gtccccac
//
LOCUS       DATMZQ010000237         3666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3291844, whole genome shotgun sequence.
ACCESSION   DATMZQ010000237 DATMZQ010000000
VERSION     DATMZQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291844"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VN496_07730"
     CDS             complement(<1..904)
                     /locus_tag="VN496_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXF16879.1"
                     /translation="MSAVVDDKRGLEPPTTYDTHPGRYQHWRLSFDGSIATLAMNVNE
                     EKGLKPGYKLKLNSYDLGVDIELYDALNRVRFEQPQIKCVIITSARERMFCSGANIYM
                     LGLSSHAWKVNFCKFTNETRNGMEDSSRHGGLKFLAAVNGTVAGGGYELALACDEILM
                     IDDRSSTVSLPEVPLLGVLPGTGGLTRLTDKKKVRRDLADVFCSTAEGVRADRAKQWK
                     LVDHIARPQQFDTVVKQRAAALAAQSDRAGTGAGIALTPLTRAIDAAGYHYAHVDVAV
                     DRKTRTATITIHAPKDPQPQNIAGI"
     gene            complement(888..1811)
                     /locus_tag="VN496_07735"
     CDS             complement(888..1811)
                     /locus_tag="VN496_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXF16880.1"
                     /translation="MGARDPKSPRETGADERRDAAFLVALGHRVRDCRVRRNLSRKGL
                     AADSGVSERYLAELESGRGNISILLLRQVSVALGIPLANLLNETDQQPAELALIQEFL
                     QRLPRARLARVRAHLLREFSSAPQGRSGRIALLGLRGAGKSTLGAALAAELEIPFIEL
                     DREIEREAGTSLSEIFLMYGQAGYRRHEYRCLEKTLENHGRCVIATGGSIVSEPATYD
                     LLLASCFTVWLKAEPAEHMARVVAQGDMRPMAGNAQAMDDLKRILQSRAALYGQADSI
                     VNTGSRSARQSLKELRRALPPEARRDHERSG"
     gene            1944..2426
                     /locus_tag="VN496_07740"
     CDS             1944..2426
                     /locus_tag="VN496_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HXF16881.1"
                     /translation="MALEFTILVGTQTGVAQLVAQEIELRLDGNDVHIKTLLMDELGP
                     GVFDGGGLFLICTSTYGQGDVPDNARRLYEGLQSERPNLSNVRYGLIGLGDNSTHAAT
                     FCFAAKKFDSILGELGAHRIGEPLFNDSCTCLPEDDAAEWIERWIPLCRAQLGVPVSN
                     "
     gene            2535..>3666
                     /locus_tag="VN496_07745"
     CDS             2535..>3666
                     /locus_tag="VN496_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXF16882.1"
                     /translation="MAGLSQADHGVSPPLITIPRDYNAAHDLVERNLLAGRGSKTAYI
                     DDNGRYSFAELGERVSRAANALTGLGLEMESRVMLALLDTIDFPSVFLGAIKAGVIPV
                     PANTLLTTADYKFMLEDSRVRALIVSEALLPAFAPLLGNVSTLKHVIVSGRDAHGHLH
                     LQQLMAAASAKCEPASTKADDACFWLYSSGSTGTPKGAVHVHSSLIQTAELYAKPVLG
                     IRETDVVFSAAKLFFAYGLGNALTFPLAVGATALLMAERPTPAAVFKRLKEHRPTIFY
                     GVPTLYGALLAHPERPIKSELALRVCTSAGEALPAELGQRWTEQFGVEILDGIGSTEM
                     LHIFISNRPGEVRYGTTGKPVPGYEVRLVDDQGNVITKPGELG"
BASE COUNT          699 a         1120 c         1092 g          755 t
ORIGIN      
        1 gaatgcccgc aatgttttgc ggttgtggat ctttgggcgc atggatggtg atcgttgcgg
       61 tgcgcgtctt gcgatccacg gcaacgtcca catgcgcata gtggtaaccc gctgcatcga
      121 tcgcgcgagt caacggcgtc agcgcaatgc cggcacccgt gccagcgcga tcgctttgcg
      181 cggccagcgc ggccgcgcgc tgtttgacaa ccgtgtcgaa ctgctgtggc cgcgcgatat
      241 gatccacgag cttccactgc ttcgcgcgat ctgcgcgcac gccttccgca gtggagcaaa
      301 acacatccgc cagatcgcgg cgcactttct tcttgtcggt gaggcgcgtc aggccgccgg
      361 tgccgggcaa tacgcctagc agcgggacct ctggaaggct gaccgtggac gagcggtcat
      421 cgatcatcag gatctcgtcg caggccaggg cgagttcata gccgccgcct gctacggtgc
      481 cgttcaccgc cgcaaggaat ttgaggccgc catgccggct ggaatcttcc atgccattgc
      541 gcgtctcgtt ggtaaacttg cagaaattca ctttccacgc atgcgaggag agcccaagca
      601 tgtaaatgtt ggcgccggag cagaacatgc gctcacgggc gctggtgatg atcacgcact
      661 tgatctgcgg ctgctcgaaa cgaacgcggt tcaacgcatc atagagttcg atgtcgactc
      721 cgagatcata agaattgagc ttcagtttgt agcccggctt gagtcccttt tcttcgttga
      781 cgttcatggc cagcgtggcg atgctgccgt caaaggacag gcgccagtgc tggtagcgcc
      841 cggggtgcgt atcgtacgtc gtgggcggct cgaggccgcg tttgtcgtca accactgcgc
      901 tcatggtctc tccttgcctc gggtggcaat gcgcgccgga gttctttgag gctctgcctg
      961 gcgctccggc tgccggtgtt cacgatgcta tctgcctggc cgtataacgc cgcgcggctt
     1021 tgcagaatgc gtttgaggtc gtccattgcc tgcgcgttcc cagccatggg ccgcatgtcg
     1081 ccctgcgcca ccacgcgcgc catgtgttca gcaggttctg ccttcaacca cacggtgaag
     1141 caagacgcga gcagcaggtc ataggtggct ggttccgata cgatgctgcc tcccgtggca
     1201 attacgcacc ggccgtggtt ttccagtgtt ttctccaggc agcggtattc gtggcggcgg
     1261 tagcccgcct gtccgtacat caggaatatc tcggacaagc tggtaccggc ctcgcgttcg
     1321 atctcacgat ccagttcgat gaatggaatt tcgagttcag ctgccagcgc cgcaccgagc
     1381 gtcgatttgc cggcaccccg cagtcccagc aaggcgatcc tgccgctgcg cccttgcgga
     1441 gcgctgctga attcgcgcag caagtgcgcg cgcacgcggg ccagccgtgc tctggggagc
     1501 cgctgcaaaa attcctgaat cagcgcgagt tcggcgggct gctgatccgt ctcgttcagc
     1561 aggttagcca acggtattcc cagtgccacg ctgacctggc gcagcaacag aatcgagata
     1621 ttgccccgac cggattcgag ttcggcaaga tatcgttcag acacgccgga atcggcggcc
     1681 aatcccttgc ggctcagatt gcgccgcacc cggcaatcgc gcacgcgatg acccagggcc
     1741 accagaaaag cggcgtcccg ccgctcgtcg gcaccggttt cccgggggga cttcggatcg
     1801 cgcgcgccca tagtttatcg atctagcatt tatcgctttg gctatactgc aatatattgc
     1861 agcaacaaaa tccaacaagc atcataatac atatcatcat actcacgata aaaatataat
     1921 gagaccttct cggtcgaata gccgtggcgc ttgaattcac catcctcgtc ggcacccaga
     1981 ccggcgttgc gcagctggtg gcgcaggaaa tcgaattgcg cctcgatggc aacgatgtgc
     2041 acatcaagac gctgctgatg gatgagctcg gtcccggcgt cttcgatgga ggcggcctgt
     2101 tcctgatctg cacatcgacc tacgggcaag gcgacgtgcc ggataacgcc aggcgtctgt
     2161 atgagggcct gcagtcggag cgtcccaatc tttcaaacgt gcgctatggc ctcatcggcc
     2221 tgggcgacaa cagcacgcac gcggccactt tctgctttgc cgcaaagaag ttcgatagca
     2281 tccttggcga actcggcgcg caccgcatcg gtgaaccgtt gttcaacgat tcctgcacct
     2341 gtttgccgga agacgatgcc gcggagtgga tcgaacgctg gatccctctg tgccgcgcgc
     2401 agttaggtgt gccggtttca aattgatgcc gatcgcaaat tggccaacat ccgccttgca
     2461 aagcgaggcc ggctggtata ttgcgtgcgg caaccggggg cagcgatgtt ttcaccaaaa
     2521 tcgaaagggt tagcgtggcc ggactgagcc aggcggatca cggcgtcagc ccgccattga
     2581 tcactattcc acgcgactac aacgcggcgc acgatcttgt cgagcgcaat ctgctcgcgg
     2641 gacgtggcag caagacggct tacatcgacg ataacgggcg atactccttt gccgaactgg
     2701 gcgagcgcgt gagtcgtgct gcaaatgcgc tgaccggtct cggcctggaa atggaaagcc
     2761 gcgtgatgct ggcgctgctc gacaccatcg attttcccag cgtttttctc ggcgcgatca
     2821 aggccggcgt cataccggtg ccagcgaaca cactgctgac aaccgccgat tacaaattca
     2881 tgctggaaga cagccgcgtc cgcgcgctga ttgtttccga agcgctgctg ccggccttcg
     2941 caccgctgct gggcaatgtg tcgacactga agcacgtcat cgtgtccggc cgcgatgcgc
     3001 acggtcatct tcatttgcaa cagctcatgg ctgcagccag cgcaaaatgc gagccggcat
     3061 ccaccaaagc ggacgacgcc tgtttttggt tgtattcctc cgggtccacg ggcacgccga
     3121 aaggcgccgt acacgtccat tccagtctga tccagaccgc cgaactgtat gcgaaaccgg
     3181 ttctgggcat tcgagaaacc gacgttgtat tctccgcagc caagctgttt ttcgcctatg
     3241 ggttgggcaa tgcgcttacg tttccgcttg ccgtgggcgc caccgcactg ctgatggcgg
     3301 aacgtcccac gccggccgcg gtattcaagc ggcttaagga acatcgaccg accatttttt
     3361 acggcgtgcc aacgctctac ggagcactac tggcgcatcc ggagcggccg atcaaaagcg
     3421 agctggcatt gcgcgtatgc acttctgctg gcgaagcact gcctgcagaa ctgggccagc
     3481 gctggacgga acagttcggc gtggaaattc tggacggcat cggttcaact gaaatgctgc
     3541 atatttttat ttcaaatcgc cccggcgagg tgcgctacgg caccaccggc aagcccgtgc
     3601 ccggctacga ggtgcggctc gtcgacgacc agggcaacgt catcaccaag ccgggcgagc
     3661 tcggcg
//
LOCUS       DATMZQ010000238         2818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3292000, whole genome shotgun sequence.
ACCESSION   DATMZQ010000238 DATMZQ010000000
VERSION     DATMZQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292000"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VN496_07750"
     CDS             <1..348
                     /locus_tag="VN496_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF16883.1"
                     /translation="ATLMFGCMVLLPLLPWWEATIPVFVVAGFGFYMFHNTLQTRATE
                     MAPQRRGTAIAVFAFSLFMGQASGVAICGVMIHLLNYGWTFGFGGISLALLGIWFAAR
                     IARHRAQIHTQPV"
     gene            complement(376..1572)
                     /locus_tag="VN496_07755"
     CDS             complement(376..1572)
                     /locus_tag="VN496_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXF16884.1"
                     /translation="MHARIRSRGNFLLYGLALTLFLVDCGGGGGDPAPPQSMPLKLQR
                     IDSSSLTFDFPVFLTAPPSDTDRLFVVEKGGRIKVIARETNTLIGIFLDIGSLVSTGS
                     EQGLLGLAFDPLYASNGRFYVNYTDTSGNSVIARYRVSADANVAAPNADGIVLTVTQP
                     FANHNGGMIAFGPDGLFYIGFGDGGSGGDPGNRAQNPNELLGKLLRIDVSQGGPSYGI
                     PPSNPLVGQNGRPEVWSLGLRNPWRFSFDRQTGDLYIGDVGQGNFEEVDVATKANGAG
                     RGVNYGWNVMEGNHCFPAGMGCNTGGFTTPQLEYDHSNGACSITGGYVYRGSAMPALQ
                     GTYFYADFCAGFVRSFRLANGMASEPLDWAALRPGGSITSFGEDAAGELYLMTSDGQL
                     NRIVQG"
     gene            1793..2485
                     /locus_tag="VN496_07760"
     CDS             1793..2485
                     /locus_tag="VN496_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HXF16885.1"
                     /translation="MTRGQLDHAGVRILWLRLRRPRLAAGVAGGILAYVLLLLLGDAP
                     GRIRIILAWDIGVLLAMILLAGLRNTSPETMKRIADRQDAGKWAVLLLTLLAATASLI
                     TIAGEVPFVRNATDLEKLWRIALIVVTIILSWTFIHTIFTLHYAHDYYSTSPVAAGKP
                     RKGLAFPGEAMPTYMDFAYFSFTIGMTFQVSDVQVTDADMRKLALTHGIISFFYATGI
                     LALTINMVASLI"
     gene            complement(2637..>2818)
                     /locus_tag="VN496_07765"
     CDS             complement(2637..>2818)
                     /locus_tag="VN496_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16886.1"
                     /translation="LTSTGSFATVAMAEEETTPAVSDTAAAMSDTAATEQSAADSAAT
                     GSDGTIMATPETDSK"
BASE COUNT          549 a          831 c          820 g          618 t
ORIGIN      
        1 gctacgctga tgtttggctg catggtattg ctgccactgc tgccgtggtg ggaggcgacc
       61 attccggttt ttgttgtcgc gggattcggc ttctacatgt ttcacaacac gctgcagacg
      121 cgggcaacgg aaatggcgcc gcaaaggcgc ggcacggcca ttgcggtatt tgcgttcagc
      181 ctgttcatgg ggcaagcaag cggcgttgcg atctgcggcg tcatgattca cctgctgaac
      241 tacggctgga cgttcggatt cggcggaatc agcctggcac ttctgggaat atggtttgct
      301 gcacgcatcg cgcggcatcg cgcgcaaata cacacgcagc cagtctaatt tcggatctgc
      361 gccaaacgcg aagcttcagc cctgcacgat gcggttcaat tgcccatccg aagtcatcag
      421 gtagagctct ccggcggcgt cctcgccaaa actggtgata cttcctcccg ggcgcaaagc
      481 ggcccagtcg agcggctcgc ttgccatacc gttcgcaagg cggaaactgc gcacaaatcc
      541 tgcacaaaaa tccgcgtaaa agtacgtccc ctgcaacgcc ggcatggcgg agccgcggta
      601 aacataaccg ccggtgatcg agcaggcgcc gttggagtgg tcatactcca gttgtggcgt
      661 tgtgaaacca cccgtattgc aacccatccc cgcaggaaag cagtgatttc cctccatcac
      721 gttccacccg taattgaccc cgcgtccggc gccgttagcc ttcgtggcga catcgacttc
      781 ttcaaaattt ccctgcccca cgtccccgat atacagatcg cctgtttggc ggtcgaagct
      841 gaaacgccag gggttgcgca agcccaggct ccagacttcc ggacgtccgt tctgacccac
      901 cagtggattg gacggtggaa taccatagga aggtccccct tgagacacgt cgatgcgcag
      961 caacttgccc agcaactcat tcggattctg cgcgcggttg cccggatctc cgccactgcc
     1021 accatcgccg aaaccgatat agaacaggcc atcgggtccg aaagcaatca tgccgccgtt
     1081 atggtttgcg aacggttgcg tgaccgtaag cacaatgccg tcagcattgg gtgcggcgac
     1141 attggcgtcg gcactgaccc gatagcgagc gatgacggaa ttaccgctag tgtccgtgta
     1201 attcacgtag aagcgcccat tgctggcgta cagcgggtca aaggccagtc cgagcaaccc
     1261 ctgttcggaa cctgttgaaa ccaggctgcc gatatcgaga aaaatcccga tcaacgtgtt
     1321 tgtttccctg gcgatcacct tgatgcgtcc gcctttttcg acgacgaaca atcggtcggt
     1381 atcactcggc ggcgcagtca ggaaaaccgg aaagtcgaac gtcagtgagg atgagtcgat
     1441 ccgctgcagc ttgagcggca tactctgtgg cggtgccgga tcgccgccac cgccgccgca
     1501 gtcaacaagg aacagcgtca gtgccagccc gtacagcaaa aagtttcccc gcgatctgat
     1561 gcgtgcgtgc atggctgcct ccctgcgcaa gtaaagagtc agtaggctga ctcttgaaac
     1621 gagcggaagt tctgggtcaa tgtctctcgc gcccgacggt gcagggcgct ggccttgttc
     1681 gcgggatccc gtcatcatgt cacgacacgg gctgcttcgt tcccacgcga ggaatttttg
     1741 catacggact gcggctgtcg gttttatttg tgcggtatgt ccggaggata cggtgaccag
     1801 gggacagctc gaccatgcag gcgtgcgcat cttatggctg cggttgcggc gcccgcgtct
     1861 cgcggcgggt gtcgccggcg ggattctggc ctacgtgttg ctgctattgc tcggcgatgc
     1921 gccgggtcgc atcagaatca tcctggcatg ggacatcggc gttttgctgg cgatgatatt
     1981 gctggcggga ctgcgaaaca cttctccaga gaccatgaag cgcatcgcag accggcaaga
     2041 cgcggggaaa tgggccgtgc tgctgctaac gttattggcg gcaaccgcaa gtctgatcac
     2101 cattgccggc gaagtaccgt ttgtcagaaa cgcaaccgat cttgaaaaac tgtggcgcat
     2161 cgcgctgatc gtggtcacga tcatcctttc ctggacgttc attcatacca ttttcacgtt
     2221 gcactacgcc cacgattact attcaaccag tccggttgcg gcgggcaaac cgcgcaaggg
     2281 actcgcgttt cccggcgagg ccatgccgac ttacatggac tttgcgtatt tttctttcac
     2341 cattggcatg actttccagg tttcggacgt ccaggtcacc gacgccgaca tgcgcaagct
     2401 ggccttgact cacggaatca tttctttttt ttacgccacc ggcatcctgg cgctgaccat
     2461 caacatggtg gccagcttga tctgacattg cgccttgatc tgaccttgcc gcctgatttg
     2521 accattggat tgcgcgcagg atgcacgatt cggaaaatgt gaccgcggtg tgacacgcat
     2581 catcctgcga ggaataaacc cgccgccgcg ggtttattgg tgttgcagcc gacggttcac
     2641 ttcgaatcag tctcgggggt cgccatgatc gttccgtcgc tgccggtggc ggcgctgtcg
     2701 gcggctgatt gctctgtggc cgccgtgtcg ctcatggctg cggcagtatc cgagactgcc
     2761 ggggtggttt cctcttccgc catggcgacc gtggcaaacg atccggtgga tgtcagca
//
LOCUS       DATMZQ010000239         6965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1476631, whole genome shotgun sequence.
ACCESSION   DATMZQ010000239 DATMZQ010000000
VERSION     DATMZQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476631"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VN496_07770"
     CDS             <1..744
                     /locus_tag="VN496_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HXF16887.1"
                     /translation="NRISRVVLGNAAHAIAGMAMHLPADAQSKPAIGLTMFGVTTSCV
                     TQVVSELQNEFDCLVFHATGTGGQSMERLADSRMLTGIIDVTTTEVCDHLFGGVLSAG
                     EDRLGAIARSRIPYVGSVGALDMVNFWAMQSVPERFRSRLLYRHNPNVTLMRTTAAEC
                     RKIGSWIADKLNACEGPVRFLIPEKGVSALDAPGQPFYDPDADAALFESLSVSLKQTE
                     TRRLMRLPNHINDPAFSAALVQQFRAIVQ"
     gene            759..1589
                     /locus_tag="VN496_07775"
     CDS             759..1589
                     /locus_tag="VN496_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HXF16888.1"
                     /translation="MPAIERKKILEKFADMVKRRVPIIGGGAGTGLSAKCEEAGGIDL
                     IIIYNSGRYRMAGRGSAAGLLAYGNANDIVKDMAREVLPVVKHTPVLAGVNGTDPFVL
                     MPHFLAELKLMGFSGVQNFPTIGLFDGIMRASFEETGMGFGLEVDMIREAHGQDLLTT
                     PYVFNPGEAQAMAQAGADIIVAHMGVTVGGNVGASSAKTLDLCVQEIDAIAEAAMTVR
                     KNVIVLCHGGPIALPADANFILKHVKTCHGFYGASSMERLPTEIALTEQTRKFKAIQF
                     "
     gene            1605..2060
                     /locus_tag="VN496_07780"
     CDS             1605..2060
                     /locus_tag="VN496_07780"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF16889.1"
                     /translation="MIKVYRSAIIHSPVDTVWKLVRDFNALPKWHPAIATSEIEGGLP
                     ADQIGCVRRFTRRRDGGLLREQLLSLGDDTRTFTYSILVSPMPVANYVASMRFTPITM
                     QHATFAEWWAVFNVTTFAQQATADDIGDNVFVAGFAAIDKLLAGRKEAQ"
     gene            2057..2503
                     /locus_tag="VN496_07785"
     CDS             2057..2503
                     /locus_tag="VN496_07785"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF16890.1"
                     /translation="MTKVFVSAVINAPVQEVWSQVRDFNNLPEWHPKFSRSHIENGLP
                     SDRVGCVRNFDIAGGGGTIRERLLELSDMDYSFRYCILTSPLPVERYVAQLTLYPVTV
                     GDKTVGIWTAEFDVTDGDEAGVVKAVADDTFGKAFEILNDRLGRKT"
     gene            2650..3852
                     /locus_tag="VN496_07790"
     CDS             2650..3852
                     /locus_tag="VN496_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXF16891.1"
                     /translation="MVSTDGQRFVGKVIEETSDSVVFESQQNGRFTFPRAQILELQLS
                     PVEEATKPPAAATGEQKSNNPEWRPPGVGHDHFDWIQLGSGEWLKGHITYIQNKEIEF
                     DSDELEELTLELKKIARLYSAEPMFVQFENHKPVYGRVEIDHENVNVLGPEYLSLHRD
                     ELTGITPSGGKTGISNWTGRFNLGLNTQSGNTEQITLNSSAKLARRTPNTVLLIDFLG
                     NFTQTSGVKTVSNERLTVSDDIRINQKWFVRAIQFEAYRDPLSNISHRFTAGVGVGYY
                     IFDRDDLEWTVSAGPGYQSTKFDEVAAGQSDSDSTPAAGIQSRFSVDITKRLTFIQTL
                     DTQFTNPESGKYTHHAVTTLEYEIKRHFNLDTSFVWDYLQNPKARSDGVIPQKSDYYT
                     SVSIGFRF"
     gene            4022..4783
                     /locus_tag="VN496_07795"
     CDS             4022..4783
                     /locus_tag="VN496_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16892.1"
                     /translation="MAASLFASSGICAEATASTPDQWQFEITPYVFAAGVKATTGVGG
                     ITADIDLSFNDIVNHLDFYGAALFEARHGRWTFGLDSFYVRMKDEKAAYRSGPVGLVG
                     ANADLNMQLTQQIYQPFLGYRLSEGKIRFDVIGGARYTNVNIDLGLTASTTAPLLPGG
                     ERNVSTTKSWWDPIVGVRADVPLMEKWSIFGYADTGIGENTTYQAIAGVKWQISNMIS
                     TSLGYRYLRQNYDSGNFHWNDATTQGAILGVGIRW"
     gene            4903..5946
                     /locus_tag="VN496_07800"
     CDS             4903..5946
                     /locus_tag="VN496_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HXF16893.1"
                     /translation="MTKSNRYPSRALFACVALATCLIAINRSGLGADAADLSRCASIK
                     HDAERLQCYDSAGRKSDAEPIERLHEPAAAPLKPASGTHPPTALSVRWELEPETKQGL
                     WALRPHQPTFFLPVRYSNSTNDSPQSPSQPVSASVPLDNTEAEFQFSLKLKAGENLFG
                     SDVDVWLGYTQQSQWQVYNGEISRPFRETDYQPEVFFSLPTRYDFLGLTGRFINLGLV
                     HQSNGRAEPLSRSWNRIYAQFGFERNGFALLVRPWYRLQEEASADNNPDIVHYMGHGD
                     VTAIYQRGRQQFSLLARYNNGYGALQGTWSFPIQERLMGYVKVFNGYGETLIDYNWNQ
                     TTIGVGILLMDWL"
     gene            6022..6636
                     /locus_tag="VN496_07805"
     CDS             6022..6636
                     /locus_tag="VN496_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16894.1"
                     /translation="MKYCLICLLALLSLVPGCATTDLTGHWKNPEYSGEPASKVLVVG
                     IADHPGVRRVFEDTLAQALTKRGVQAVASYSVVPTRGKISEEELVKAVERTGVNGVLI
                     TRLVRRETETLYAPEPMGMAPAAYGMSTRYYGYYDAAWSDHYEPAMAQTIEYVIAETA
                     LFRPDVQKPVWSGTTRSQRMGDVRKATEDFAEVMIDAMKKDGVI"
     gene            complement(6722..>6965)
                     /locus_tag="VN496_07810"
     CDS             complement(6722..>6965)
                     /locus_tag="VN496_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF16895.1"
                     /translation="MRRRIFSLYGELKFLEADLDSRGPRAPADDLRLALDKLEFKANH
                     MRVPVTFAHMLYTLRIHISLVRNELSQRTRPAVSKD"
BASE COUNT         1592 a         1906 c         2000 g         1467 t
ORIGIN      
        1 aaccgcatta gccgagtcgt gctggggaac gctgcacacg cgattgccgg aatggcgatg
       61 cacctgccgg ccgacgcaca gtcgaagccg gcgattggac tgacaatgtt tggcgtgact
      121 accagctgtg tgacgcaggt ggtgtccgag ctgcaaaacg aatttgactg tctggtgttt
      181 catgccacag gcacgggcgg ccaatccatg gaacggctgg cagacagccg catgctaact
      241 ggcatcatag atgtcaccac caccgaggtc tgcgatcatt tgttcggcgg cgtactgtcc
      301 gccggcgaag atcgtttggg tgccatcgcg cgtagtcgca tcccttacgt cggttcggtg
      361 ggtgcgctcg acatggttaa tttctgggcg atgcaatccg tccccgagcg tttccgttcg
      421 cgcctgctat acaggcacaa tcccaatgta acgctaatgc gaacgacggc cgccgaatgc
      481 cgcaagatcg gcagctggat tgccgacaag ctcaatgcat gcgaaggtcc ggtgcgtttt
      541 cttattccgg aaaaaggcgt ttccgcactg gatgcaccag gccagccatt ttacgatccg
      601 gacgccgatg cagcgctgtt cgaatcactg tcagtttcat tgaagcaaac tgaaacgcgc
      661 cgtctcatgc gactgccgaa ccatatcaac gacccggctt tctccgccgc actggtgcag
      721 cagtttcgcg caattgtcca ataattcagg gaaccgccat gccggccatt gaacggaaaa
      781 aaatactgga gaaattcgcg gacatggtca agcgccgcgt gcccatcata ggcggcggcg
      841 cgggaaccgg cctgtcggcg aaatgcgaag aagccggcgg catcgacctc atcatcattt
      901 acaattccgg ccgctatcgc atggcggggc gcggctcggc agccggcctg cttgcatacg
      961 gcaatgccaa cgacatcgtt aaggacatgg cgcgcgaagt tctaccggtc gtaaagcaca
     1021 cgcctgttct cgccggggta aacggcacag atccttttgt actgatgcct cactttctgg
     1081 ccgagttaaa gcttatgggg ttttccggtg tgcaaaactt tccgaccatc ggactattcg
     1141 atggcatcat gcgagcgagc ttcgaagaga ccggcatggg ttttggactc gaagtcgata
     1201 tgatccgtga agcgcacggg caggatttgc tgaccacgcc ctatgttttc aatcccggcg
     1261 aagcgcaggc aatggcgcag gccggcgccg acatcatcgt tgcgcatatg ggtgtcacgg
     1321 tcggtggcaa tgttggtgcg tcttcggcca aaaccctgga tttgtgcgtg caggaaatcg
     1381 acgcaatcgc agaagccgcc atgacggtgc gtaagaatgt aatagtgctc tgtcacggcg
     1441 gtccgattgc gctgcctgcg gacgccaatt tcattttgaa gcatgtgaag acatgtcacg
     1501 gcttttacgg ggccagcagc atggaacggc tgccgaccga aattgcactg actgaacaga
     1561 cacgcaagtt caaggcaatc cagttctgac aacaggagat accgatgata aaggtgtatc
     1621 gaagcgccat catccattca ccggttgata cggtatggaa gctggtgcgc gacttcaatg
     1681 cactgccgaa atggcatccc gcgattgcga ccagtgagat cgaaggcgga ctgccggcgg
     1741 accagatcgg ctgcgtacgc cgctttaccc gccggcgtga tggcggcttg ctgcgggagc
     1801 aactgctttc gctgggggac gatacgagga cattcaccta cagcattctg gtgtcgccca
     1861 tgccggtagc aaactatgtt gcatcaatgc gcttcacgcc gatcaccatg caacatgcca
     1921 cgttcgccga atggtgggcc gtcttcaatg tgacgacctt tgcgcagcag gccaccgccg
     1981 acgatatcgg cgacaacgtc ttcgtagcgg gattcgcggc tatcgacaag ctgctggcgg
     2041 gccgaaagga ggcgcaatga caaaagtatt cgtgagcgcc gtgattaatg caccggtgca
     2101 ggaagtctgg tcgcaagtgc gcgacttcaa caacctgcct gagtggcatc ccaaattctc
     2161 gcgcagtcac atcgaaaacg ggctgccgag tgatcgtgtt ggttgcgtgc ggaactttga
     2221 cattgccggt ggcggcggaa cgattcgcga acgtctgctc gaattgtcgg acatggacta
     2281 tagttttcgc tactgcattc tgacctcccc gcttccggtc gaacgctacg tggcgcagct
     2341 gacgttgtat cccgtcaccg tcggcgacaa gaccgtggga atctggacgg cagaattcga
     2401 cgtgaccgac ggagatgagg caggcgtggt gaaggctgta gcagacgata cctttggcaa
     2461 ggcatttgaa atcctgaacg accgcttggg ccgcaagact tagggcgcgg cactttgcgg
     2521 actattttcg agcatcgaga gccgcctcga aacagacgcg gacggtcctg taagggccgc
     2581 tggaataccg tcctggtttg cctggcattc gcgagtgcct cgctgccggt tggcgcgctg
     2641 gccgatgtct tggtcagcac cgatggccag cgattcgtgg ggaaagtgat cgaagaaacc
     2701 agcgatagtg tcgtgttcga gtcgcagcaa aatggcagat tcactttccc gcgcgcgcaa
     2761 atccttgaac ttcagctatc gccagtggag gaagcaacaa agccgccggc tgccgcgacg
     2821 ggtgagcaaa aatcaaacaa tcccgaatgg aggccacccg gggtgggcca cgaccatttc
     2881 gattggattc agctggggtc gggagagtgg ctcaaagggc acatcacgta cattcagaac
     2941 aaggaaatcg aatttgacag tgacgaactg gaagaactca cgctggagtt gaaaaagata
     3001 gcgagacttt attccgcgga accgatgttc gttcaatttg agaaccataa gccagtttac
     3061 ggtagagtcg aaatcgatca tgagaacgtc aatgtcctgg ggcctgaata tttaagcctg
     3121 caccgcgatg agcttaccgg catcacgccg agtggcggaa aaacgggcat cagcaactgg
     3181 acgggcagat tcaatctcgg attgaacacg cagtccggga acaccgagca gattacactg
     3241 aactccagtg caaaattagc gcggcgaacg cccaataccg tcttgctcat cgacttcctg
     3301 ggaaatttca cgcagaccag tggcgtcaaa accgtcagca atgagcggct caccgtgagc
     3361 gatgacatcc gcattaacca aaagtggttt gtccgcgcga ttcaattcga ggcttatcgg
     3421 gatccgttgt ccaacatctc ccatcggttc actgccgggg ttggagtggg ctactatatt
     3481 tttgaccgcg acgatctgga atggactgtg tccgccgggc cggggtacca atccaccaaa
     3541 tttgatgaag tggcggccgg acaaagtgat tccgattcca cgccggcagc aggaatccag
     3601 tcacgtttca gcgttgatat caccaagcgg cttacgttca tccagacgct ggacacccaa
     3661 tttaccaatc cggagtcggg caaatatact catcacgcag tcaccacgct ggaatacgag
     3721 atcaagcggc atttcaatct ggatacttct tttgtctggg attatcttca gaatcccaag
     3781 gcaagatcgg acggcgtcat tccacaaaaa agtgattact acaccagtgt cagcatcgga
     3841 ttcaggtttt agaagtagcc gggcgttgga gcacaagcgc cttttggccg cgcttgatct
     3901 gagtcaatgg caaccagtgg catgtgttat ggttaaggtg aaaaaatcgg atcaaggaca
     3961 aaagatggcc atgcgaaaat taaacggaaa tgaaagctgt tttggacgaa tcgcgtgttg
     4021 tatggctgca agcctttttg catcttcggg aatctgcgca gaggccactg cgtcgacccc
     4081 ggaccaatgg cagttcgaaa taactcccta tgtgttcgcc gccggggtga aggccaccac
     4141 cggcgtgggt gggataacgg ccgatatcga tttgagcttc aacgacatcg tgaaccacct
     4201 ggatttttat ggagcagcgc tgttcgaggc tcgccacggg cgctggacgt ttggcctcga
     4261 tagcttttac gtgcgcatga aggatgaaaa agccgcctac cgcagcggcc ctgtgggcct
     4321 tgtaggggcc aatgccgatc tcaatatgca attgacccag cagatctacc agccttttct
     4381 cgggtaccgg ctgtccgagg gcaaaatcag atttgatgtc atcggcgggg cacgctacac
     4441 caacgtaaat attgatctgg gcctcaccgc ctcgacaacg gcgcctttgc tgcctggcgg
     4501 cgagcgtaac gtcagcacca ccaagagttg gtgggatccg atcgtcggcg tacgcgcaga
     4561 cgtgccgttg atggaaaaat ggtccatctt cgggtatgcc gacactggca ttggagaaaa
     4621 cacgacttac caggccatcg ccggcgttaa atggcagatc tcgaacatga tttcgacgtc
     4681 gcttggctat cgctacctgc gccagaatta cgatagtggc aactttcatt ggaatgacgc
     4741 cacaacgcag ggcgccatcc tcggggtagg tatccgctgg tagcacaccg gattgcgctc
     4801 cttgatagtc agcagaatgg tgctgatgcc gcgcatgccg ggactgtaag caattctgcg
     4861 ccggttctgt atctcttctg agaacagaaa caaggtatat cgatgacgaa atcgaatcgg
     4921 taccccagcc gggcattgtt tgcatgtgtc gcgctcgcaa cttgcctcat cgcaatcaat
     4981 cgctccggtc tcggagccga tgcggcggat ctatcgcgct gtgcctcgat caagcatgat
     5041 gccgagcgtc tgcaatgcta tgactctgcg ggccgaaaga gcgacgccga accgatcgag
     5101 cgtttgcacg agcctgcggc tgcccctcta aagccggcgt ccggcacgca tccgccgact
     5161 gcgctctcgg tacgctggga actcgaaccc gagacaaagc agggattgtg ggcgttgcgc
     5221 cctcatcagc cgacattttt cttgccggtc cgctacagca acagcaccaa cgactcgccg
     5281 caatcgccgt cgcagcctgt gtccgccagc gtacctctcg acaacaccga agccgaattt
     5341 cagttcagcc tgaaattgaa agccggtgaa aacctgtttg gcagcgacgt cgatgtgtgg
     5401 ctcggctaca cccagcagag ccaatggcag gtctacaacg gcgaaatctc ccgcccattt
     5461 cgcgaaaccg actaccagcc cgaagtattt ttctcgctcc ccacacgcta cgatttcctt
     5521 gggctcaccg gtcggttcat taatcttgga ctggtgcacc agtccaacgg ccgggccgaa
     5581 ccgttgtcgc gcagctggaa tcggatctat gctcaattcg gattcgagcg aaacggcttc
     5641 gccctgctgg tacggccctg gtaccgtttg caggaggaag cctcggccga taacaatcca
     5701 gacatcgtgc actacatggg ccacggcgat gtcacagcga tctaccagcg tggacggcag
     5761 cagttctcac tgctggcgcg gtacaacaac ggttacggtg cgctgcaggg cacctggagt
     5821 tttcccatac aagaacgctt gatgggttac gtcaaggtct tcaatggtta tggcgaaaca
     5881 ttgatcgact ataactggaa ccagaccaca atcggcgttg gcattctgct gatggactgg
     5941 ctgtagcgcc aacttgggtc ctggcgcatc caagcatacc gtacgctatt ccattagctt
     6001 tgccatcgga gaaaactgct catgaaatat tgcctgatct gcttgttagc cctgctgtca
     6061 cttgtgcccg gttgtgccac aaccgatttg accggccact ggaaaaatcc agaatactcg
     6121 ggagaaccgg ccagtaaagt cttggtcgtc ggcattgccg atcacccggg ggtgcgccgc
     6181 gtattcgaag atacccttgc gcaagccctt acaaaacgcg gtgtacaggc cgttgcaagt
     6241 tacagcgtgg ttcccacgcg cggcaagatt tcggaggagg aattggtgaa ggccgtagaa
     6301 cggacgggcg taaacggagt gctgatcacg cgtttggtcc gcagggaaac tgaaacgtta
     6361 tatgcgcccg agccgatggg gatggctcca gcggcgtacg gcatgagcac gcgctactac
     6421 ggttattatg acgcggcctg gagcgatcac tacgaaccgg cgatggcgca aaccatcgaa
     6481 tatgtcatcg ccgaaacagc attgttccgt cccgatgtgc aaaagccggt ctggtctggc
     6541 accacgcgtt cgcagagaat gggggacgtg cgcaaggcaa ccgaggattt tgctgaagtg
     6601 atgatcgacg caatgaaaaa agatggcgtc atttgagcgc tctgcggcaa tgcagccact
     6661 cgtgtcaagg ccctgacctg catgcgcggt acctgtgtca acatgcgaat ttgcatcgcg
     6721 gtcagtcttt gcttacggct ggacgcgtcc gctgcgatag ctcattgcga accagactga
     6781 tatgaatgcg caaggtgtag agcatgtgcg cgaatgtcac cggcacccgc atgtgattcg
     6841 ccttgaattc aagcttgtcg agagctaagc gaagatcgtc agccggcgct cgcggcccgc
     6901 gcgaatcgag atctgcttcc aggaatttga gttcaccata gagactaaag atgcgccggc
     6961 gcatt
//
LOCUS       DATMZQ010000240         7771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3292554, whole genome shotgun sequence.
ACCESSION   DATMZQ010000240 DATMZQ010000000
VERSION     DATMZQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292554"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..763
                     /locus_tag="VN496_07815"
                     /pseudo
     CDS             1..763
                     /locus_tag="VN496_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            866..1438
                     /locus_tag="VN496_07820"
     CDS             866..1438
                     /locus_tag="VN496_07820"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HXF16896.1"
                     /translation="MSATNQSRIKPAIEPVHLTDGTAVCSFPPIESHAARVLVLGSMP
                     GTASLRAGQYYAYRHNAFWPIMGALFGAGPEIAYEKRIVILRRAGLAFWDVLASCARE
                     GSLDAAIDEESIVPNDFRTFFDAHPNVQYVFFNGSKAESSFNRYVRPNLSERSLHFTR
                     LPSTSPAHASLPLSRKLQAWRAVAQALAKK"
     gene            complement(1495..1686)
                     /locus_tag="VN496_07825"
     CDS             complement(1495..1686)
                     /locus_tag="VN496_07825"
                     /inference="COORDINATES: protein motif:HMM:NF016234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HXF16897.1"
                     /translation="MLQQLKIRVGQLWHYLREVTGDDAYERYVAHHELAHGGQPCLTR
                     RQFFTRRQEEKWSKVCRCC"
     gene            complement(1694..3772)
                     /locus_tag="VN496_07830"
     CDS             complement(1694..3772)
                     /locus_tag="VN496_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HXF16898.1"
                     /translation="MPKTLLSKLMWFAIAIGGAAAIGGIALHRGESINAIWFIAAAIC
                     TYLVAYRFYSAWICAKVLVLDETRATPAERFNNGRDFMPTNKWVTFGHHFAAIAGPGP
                     LIGPTLAMQFGYLPGTLWILAGAVVGGCVQDMVIMFCSTRRDGRSLGQMARDELGPVG
                     GWAAMVGTMLIMLILIAVLGLVIVNAMKHSPWATATVLGTIPVAMLIGIYMRGIRPGR
                     VLEGSLIGIALLLVCVVGGGWVDHHEAIRGAFDWSGESVAWFVMIYGFAAATLPVWLL
                     LAPRDYLSTFLKLGVVFLLAASILILAPDIKMPALTQFIDGTGPIFKGALFPFVFITI
                     ACGAISGFHSLVSSGTTPKLLPNERDIRMIGYGSMALESFVGIMAVIAACVLDPGVFF
                     AINSPAGVVGKEAAAAVATISSWGFPVSVDQMNMLAHEVGEKTLFARTGGAPSLAVGM
                     ATIFSSTFGNTLLSMWYHFAIMFEVLFILTTLDAGTRVGRFMLQDMLRHVWKPIGNVS
                     SYPATLVASLLFVAAWGYFLWQGVKDPLGGVNILWPLFGISNQLLAGIALSVATGILI
                     KMGKARFAWVTGLPLTWLAVVCTTAAVEKVFSPNPAIGFLSGAASMADKLAAGTLPPE
                     RAAVAPQLIIGQQIDAALALFFAIVLWVVIFDMIRMSVRMRSGKNVLPLSEARYIRTQ
                     LDPNMLTSAH"
     gene            3986..5233
                     /gene="glyA"
                     /locus_tag="VN496_07835"
     CDS             3986..5233
                     /gene="glyA"
                     /locus_tag="VN496_07835"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXF16899.1"
                     /translation="MFSQSQTIQEFDAELWQALQSEHQRQEDHIELIASENYTSPRVL
                     QAQGSVLTNKYAEGYPGKRYYGGCEFVDIAEQLAIDRAKALFKADFANVQPHSGSQAN
                     QAVYAAALKPGDTILGLSLASGGHLTHGSPVNLSGKLYNAVAYGLNEKEEIDYEQVES
                     LARQHKPKMIVAGASAYALTFDWKRFREIADAVGAYLFVDMAHYAGLVAAGIYPSPVG
                     IADFVTTTTHKTLRGPRGGIILAKLQHEKIINSAIFPGIQGGPLMHVIAAKAVALKEA
                     MEPAFRAYQTQVISGARMMAKTLQQRGLRIVSGRTDCHMFLLDLRARKITGKDAEAAL
                     GRAHITVNKNAIPNDPEKPFVTSGVRIGSPAMTTRGFKDAEAAKLANLIADVLEAPND
                     EANIGRVRTAVMQLCKQFPVYGK"
     gene            5299..5772
                     /gene="nrdR"
                     /locus_tag="VN496_07840"
     CDS             5299..5772
                     /gene="nrdR"
                     /locus_tag="VN496_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXF16900.1"
                     /translation="MKCPFCKHPDTQVVDSRVNDEGDSIRRRRRCLSCDKRFTTYEHA
                     ELRLPQIVKSNGFRELFDEAKLRVGFNRALHKRPVSADLVDEAIERVKQKLLSLGERE
                     VDSRRLGEMVMRELYKLDKVAYIRFASVYRDFQDAEDFRNAVKEVQKPASRRKTR"
     gene            5772..6869
                     /gene="ribD"
                     /locus_tag="VN496_07845"
     CDS             5772..6869
                     /gene="ribD"
                     /locus_tag="VN496_07845"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXF16901.1"
                     /translation="MSFSAEDFQWMAQALRLAERGLYTTTPNPRVGCVLVNKAGRVIG
                     EGWHQRAGEAHAEIVALHAAGAAAADATVYVNLEPCSHHGRTAPCADALIAARVKRVV
                     AAMQDPNPQVAGGGFAKLRAAGIVTETGVLEEQAREVNIGFVSRMTRARPWMRVKIAA
                     SLDGKTGLKNGTSQWITGPDARRDAHHWRARSCAVMTGIGTLRDDDPRLTVRDVDSAR
                     QPLRVVVDSHLRIAPQANILDGGAVLVATATHDPIKAKALAAKGAAVVELPNPEGKVD
                     LLRLVEHLGELGMNEVLVEAGMNLDSALLRAGVVDELLIYMAPHMLGHAARGMLDLGE
                     LEHMKQRLNLLVRDTRSFGDDVRILARIKTH"
     gene            6870..7460
                     /locus_tag="VN496_07850"
     CDS             6870..7460
                     /locus_tag="VN496_07850"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXF16902.1"
                     /translation="MFTGIVQAVGRIENVKAAGRTAALTIHAGQLDLTDVAVGDSIAC
                     NGICLTVTALKPDGFSVDVSQETLKVCAGLIANAPVNLEKSLRLADRLDGHLVTGHVD
                     GSGEIRTVEPVEGNNEITIRFPASLARYIARKGSIAVNGVSLTVNALTNDTFSVNLIP
                     HTLAMTNLGALKPGDRVNLEIDLLARYVERILNAAE"
BASE COUNT         1735 a         2227 c         2329 g         1480 t
ORIGIN      
        1 ccttgcgctc ggtgtgcggg aggccaaata cgatcgcttc gacatgaaaa aagtgctgga
       61 gcgtttgctg gtcaaaggca gcatcgtggt caaaaaggcc tattgcgatt gggagcgcta
      121 caaggaattc aaggcgccga tgcatgaatc ggcgttcgaa atgatcgaaa ttccgcatat
      181 ccggcaatcc ggcaagaatt cagccgacat ccgtatggtc gtggatgcgc tggacctgtg
      241 ctacaccaaa tcgcacgtcg atacgttcgt gatcctgagc ggagattctg atttttcgcc
      301 gctggtctcc aagctgcgcg agaacaacaa ggaagtcatc ggtgtcggcg tcaaacactc
      361 gacttccgat ctgctcattg cgaattgcga tgaatttatt ttttacgacg acctcgtgcg
      421 caaggaaacg ccgaaacgcc cgccgcgcaa acgtgtcagc gcaaagcctg cgactggcgc
      481 aaaggtgtct ccgcccgaag ccccggatga ttccccgctg gacgacaaga cgcagaaggc
      541 catggacctg ctgatgaaca ccttcgatgc gttgaccgga gagcgtggag gcgatgacaa
      601 gatctgggga tcaatgatca agcaggcgat gaagcgccgc aatcctggct tcaatgaggc
      661 ctatcatggt tttaaaagct tcaacagctt gcttgaagag gccgccattc gcggcttgct
      721 caggctcgag cacgatgaga aatcgggcgg ctatattgtc cgggccgcgg aaccggcaga
      781 agtctgatgc tgaattgact acagcatgga cgtggtccaa tgggtcttag caacagatta
      841 gaattgcacc agtgaacaga ttcgcatgtc cgcgacgaat caatccagaa taaaaccagc
      901 aattgagccg gtgcatctga ctgacggcac agctgtatgc agtttcccgc cgatcgaaag
      961 ccatgcggcg cgtgttcttg ttcttggcag catgccggga acagcttcgt tacgcgcggg
     1021 ccaatattac gcgtaccgtc acaatgcgtt ctggcccatc atgggggcgt tgttcggtgc
     1081 cggacccgag atcgcatatg aaaagcggat tgtgattctg cgccgtgccg ggcttgcgtt
     1141 ctgggatgtg ctggcttctt gcgcgcgcga aggcagcctg gatgcggcta tcgatgaaga
     1201 atcgattgtg ccaaatgatt tccgcacgtt tttcgacgcg cacccgaacg tacagtatgt
     1261 gttcttcaat ggctcgaaag ccgaatcatc cttcaatcgc tatgtgcggc caaacctcag
     1321 cgagcgcagc ttgcacttta cgcgtctgcc gtcgaccagc ccggctcacg catcgctgcc
     1381 gttgtcgcgc aagctgcaag cctggcgcgc tgttgcgcaa gcgctggcga aaaaatagcg
     1441 cctgccggtc gcaggcgcta ttttggtctg cccctcagtc tctgtcctca gtccttagca
     1501 gcagcggcag accttgctcc atttctcttc ctgccgtctg gtaaaaaact gtcgccgggt
     1561 caaacacggc tggccgccat gcgcgagttc gtgatgcgcg acatagcgct cataggcatc
     1621 gtctccggtg acttcgcgca ggtagtgcca cagctgcccg acacgaatct tgagttgttg
     1681 cagcatgatt gcctcaatgt gccgatgtga gcatgttcgg atcgagctgc gtccggatgt
     1741 accgcgcctc ggacagtggc agcacattct tgccggagcg catgcgcacg gacatgcgga
     1801 tcatgtcgaa aatcacgacc cacagcacga tggcaaaaaa cagtgccagc gcggcatcga
     1861 tctgctggcc gatgattagt tgcggcgcca cggccgcgcg ctccggcggc aacgtcccgg
     1921 cagccaactt atccgccatg ctcgcggcac cggacagaaa gccgatggca ggatttgggc
     1981 taaacacttt ttcaaccgcc gccgtagtgc ataccactgc gagccatgtg agcggcagtc
     2041 cggtgaccca ggcgaagcgc gcttttccca tcttgatcag gatgcctgtc gccacggaaa
     2101 gagcgatgcc cgcaagcaac tggttcgaga tgccgaacag cggccacagg atattgaccc
     2161 cgccgagcgg atctttgacg ccctgccaga ggaaatagcc ccaggcagcg acgaacagca
     2221 aactcgctac aagcgtcgca ggataggacg agacattgcc gatcggtttc cagacatgcc
     2281 gcagcatgtc ctgcaacatg aagcgcccca cgcgcgtgcc cgcatccagc gttgtcagga
     2341 tgaacagcac ttcgaacatg atcgcgaagt gataccacat ggacagcaac gtattgccga
     2401 atgtgctgct gaaaatggtc gccatgccga ccgcgagcga cggcgcgccg cccgtgcggg
     2461 cgaacagggt cttttcacca acctcatgcg ccagcatatt catctggtcc acgctgaccg
     2521 ggaaccccca ggaggaaatg gttgcaaccg cggccgctgc ttctttgccg accacgccgg
     2581 ccggactgtt gatcgcgaag aatacgccgg gatccagtac gcaggctgca atcacggcca
     2641 tgatgccgac gaaggattcc agcgccatgg acccataacc gatcatgcgg atgtcacgtt
     2701 cattgggtag cagcttgggc gtggtgccgg acgaaaccag cgaatggaaa ccggatatcg
     2761 ccccacatgc gatggtgatg aatacgaacg ggaacagcgc gcccttgaaa atcgggccgg
     2821 tgccgtcgat gaattgcgtc agtgctggca tcttgatgtc tggcgcaagg atcaggatcg
     2881 acgccgcgag caggaacacg acgcccaatt tcaggaatgt cgacagatag tcgcgcggcg
     2941 ccagcagcag ccacaccggc aatgtcgccg ctgcaaaccc atagatcatc acgaaccagg
     3001 ccaccgattc gccgctccag tcgaacgcgc cgcgaatggc ttcatggtgg tcaacccagc
     3061 cgccaccgac gacgcagacc agcaacagcg caataccgat cagggaacct tccagcactc
     3121 gacccggccg aatgccgcgc atgtaaattc cgatcaacat cgccaccggt atggtgccga
     3181 gtaccgtggc agtcgcccac gggctgtgct tcatggcatt gacgatgaca agcccaagca
     3241 cggcgatgag gatcagcatg atcagcatgg tgccgaccat ggctgcccag ccgcccaccg
     3301 gacccagttc atcgcgcgcc atctgaccca gactgcggcc gtcgcgacgg gtggagcaaa
     3361 acatgatcac catatcctgc acgcagccgc ccaccaccgc acccgcgagg atccacagcg
     3421 tgccggggag atagccaaac tgcatggcca gcgtgggacc gatcaacggc ccgggaccgg
     3481 cgatcgcagc gaagtgatgg ccgaatgtca cccacttgtt ggtcggcatg aagtcgcggc
     3541 cgttgttgaa tcgttctgca ggcgttgcgc gggtctcgtc cagtaccagc acttttgcgc
     3601 agatccacgc gctgtagaaa cgatacgcga caaggtacgt gcatattgcg gcagcaatga
     3661 accagatggc gttgatcgat tcgccgcgat gcagggcgat gccaccgatt gcagcagcgc
     3721 ccccaatggc aatggcaaac cacatgagct tgctcagaag ggtctttggc atgcttcctc
     3781 cggatattta ttgctggcgt ccggtgcagc cgcaatgcgg cgccgggcgt tcggctgtat
     3841 gggtccgggc aataatagca gcaaggcctg tggccaacac catgcggcgc ggcaggacag
     3901 ccagtccgtg aaaggggcgt gagccagggg tgcaatggta caattcgcgc ctttccgaag
     3961 cgccaaccat ttttatttac atcccatgtt ttcccagagc cagactatcc aggaattcga
     4021 cgccgagctt tggcaagccc tgcaatcgga gcaccagcgt caggaagatc acatcgagct
     4081 gatcgcatct gaaaattaca ccagcccccg tgtgctgcag gcgcaaggtt cggtgctgac
     4141 gaacaaatat gccgaaggct atccgggcaa gcgctactac ggcggctgcg agttcgttga
     4201 tatcgcggag cagctggcaa tcgatcgtgc caaggcattg ttcaaggcgg atttcgcaaa
     4261 cgtgcagccg cattcaggct cgcaggccaa ccaggccgtc tatgccgctg ccctgaagcc
     4321 cggtgacacg attttgggtc tctcgcttgc gtccggcggc catttgacgc atggctcgcc
     4381 ggtgaatttg tccggcaagc tgtacaacgc agtggcgtat ggactgaacg aaaaagaaga
     4441 gattgattat gaacaggtcg aaagcctggc gaggcagcat aagccgaaga tgatcgtggc
     4501 cggcgcttcg gcatacgcac tgacgttcga ttggaaacgc ttccgcgaaa ttgccgatgc
     4561 agtgggtgcg tacctgtttg tcgatatggc gcattacgcc gggctggtcg cggccggcat
     4621 ctatcccagc ccagtcggca ttgcggactt cgtcacgacg accacgcaca agacactgcg
     4681 ggggccgcgc gggggcatca tcctggccaa gctgcagcac gaaaaaatca ttaactccgc
     4741 catttttccg ggcatccagg gcggaccgct gatgcacgtc attgccgcca aggccgtggc
     4801 gctgaaggaa gccatggagc ccgcgtttcg cgcttaccag acacaagtga tctcaggtgc
     4861 acgcatgatg gcgaagacct tgcagcagcg tggattgcgc atcgtctccg gccgcaccga
     4921 ctgccacatg ttcctgctgg acctgcgcgc ccgaaaaatt accggcaagg atgcggaagc
     4981 cgcgctaggc cgagcgcata tcacagtcaa taagaacgcc attccgaacg atccggagaa
     5041 accttttgtg acgagcggtg tgcgcatcgg ctcgcccgcc atgacgacgc ggggcttcaa
     5101 ggacgcggag gccgcaaaac tggcaaatct gatcgccgac gtgctggaag cgcccaacga
     5161 tgaagccaac atcggccggg tacgcactgc agtaatgcag ttatgcaagc agtttccggt
     5221 ttacggaaaa tgaattgcca acaaaacgga aaccaaaatc gccagccacc gcgtccatag
     5281 ccgccagccg ctgctttcat gaaatgtccc ttctgcaagc atcccgatac gcaagtcgtt
     5341 gactcgcgcg tcaatgatga aggagacagc atccggcggc gccggcgctg cctgtcgtgc
     5401 gacaagcgct tcacgacgta cgaacacgcg gaattgcgcc tgccgcaaat cgtgaagtca
     5461 aatggctttc gcgaactgtt cgacgaggcg aagttgcgcg tgggtttcaa tcgcgcgctg
     5521 cacaagcggc cggtgtccgc tgatctggtt gatgaggcca tcgaacgggt caagcagaaa
     5581 ttgctgtctc ttggcgaacg tgaggtcgat tcgaggcgac tgggcgaaat ggtcatgcgt
     5641 gagctttaca agctcgacaa ggtggcttac atccggttcg catcggtata ccgcgatttc
     5701 caggacgcag aagactttcg caacgcggtg aaggaagttc agaaacccgc ttcgcgccgc
     5761 aaaacccggt aatgtcgttc agtgccgagg atttccagtg gatggcgcaa gcgctgcggc
     5821 ttgccgaacg cgggttgtat acgaccacgc ctaatccgcg cgtgggatgc gtgctggtca
     5881 acaaagcggg gcgcgtgata ggcgagggct ggcaccagcg cgcgggcgaa gcgcatgcgg
     5941 agattgtcgc gcttcatgcc gcgggcgcgg cggccgcgga cgcgacagtc tacgtaaacc
     6001 tcgagccctg cagtcaccat ggacgcaccg cgccgtgcgc tgatgcgctg atcgccgcga
     6061 gggtcaagcg cgtggtcgcg gcgatgcagg atccgaatcc gcaggtcgcc ggtggcggtt
     6121 tcgcgaaatt gcgtgcggct ggcatcgtga cggaaaccgg tgtgctcgaa gaacaagcac
     6181 gcgaggtgaa catcggtttc gtttcccgca tgacacgcgc aaggccatgg atgcgcgtca
     6241 aaatagccgc cagcctggat ggcaagaccg ggctgaagaa tggcaccagc cagtggatca
     6301 ccggcccgga cgcgcgccgc gatgcgcatc actggcgtgc gcgctcttgc gcagtcatga
     6361 cgggcattgg cacgctcagg gatgatgatc cgcggttgac cgtgcgcgat gtcgacagcg
     6421 cacgccagcc gttgcgcgta gtcgtcgaca gccatctgcg catcgcgccg caggccaata
     6481 tacttgatgg cggtgcggtt ctggttgcaa cggcgaccca tgacccgatc aaagccaaag
     6541 cgctggccgc aaaaggcgcc gcggtagttg agttgccaaa tcccgaaggc aaggtcgatc
     6601 tgctgcgact ggtagagcat ttgggagaac tgggtatgaa tgaggtactg gtcgaggccg
     6661 ggatgaatct tgactccgcg ctgttgcggg ccggagtcgt ggacgaactg ctgatttaca
     6721 tggcgcccca catgcttggc catgctgcgc gcggcatgct cgatctcgga gaattggaac
     6781 acatgaagca gcgcctgaat ttgcttgtgc gcgatacacg ttccttcggc gacgatgtgc
     6841 gcatactcgc gcgtatcaag acacactaaa tgtttaccgg catagtccag gcggtcggcc
     6901 gcattgaaaa cgtaaaggca gcgggccgaa cggccgcatt gaccatccac gctggacagc
     6961 ttgacctcac cgacgtagcg gtcggggaca gcatcgcctg caacggcata tgcctgaccg
     7021 tgaccgcact gaagccggat ggattttcag tcgatgtatc gcaggagacg ctcaaagttt
     7081 gcgcggggtt gattgccaac gcacccgtca atctggaaaa gtcgctgcgt ctggcagacc
     7141 ggctcgatgg gcatcttgtc accggtcacg tggatggcag cggagaaatc agaacagtcg
     7201 agccggtcga ggggaataat gaaatcacaa tccgttttcc ggcgagtctc gcgcgctaca
     7261 ttgcacgcaa gggatcgatt gcggtgaacg gtgtcagcct gaccgtcaat gccttgacga
     7321 acgatacttt ttcagtgaac ctgattccac atacgctggc catgacaaat ctcggtgcac
     7381 tcaagcctgg tgaccgcgtc aatcttgaaa ttgatttact ggcgcgttac gtcgagcgga
     7441 tcctgaatgc cgcggagtag tctcacctag ggataaatcc atggcgagtt agctctgaat
     7501 ctggtatttg ttagattggt atcggctgat ttgcgctgca cagcatgaca gccatagcct
     7561 caaccaagct gaatgaatgg gatgtgggcc ggatgtgaac tgaccggcaa ccggattggc
     7621 gacacaaaca agcaaagaaa agagggcaac cgtagatcgg agtgatcaat ggaaaaagta
     7681 ggttactgtt tctggttggg aagcatggtt gcgttggccg catcctggcc aacagtaatc
     7741 tggctatttg ccatcggcaa ggggggcatt g
//
LOCUS       DATMZQ010000241        21151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4928119, whole genome shotgun sequence.
ACCESSION   DATMZQ010000241 DATMZQ010000000
VERSION     DATMZQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4928119"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VN496_07855"
     CDS             complement(<1..812)
                     /locus_tag="VN496_07855"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HXF16903.1"
                     /translation="MTTLKAYRIFSEDSKVAGRYVDMRLDELDAGEVVIKVAYSSVNF
                     KDALAATGAGKIIRRFPCVGGIDLSGTVVESADVRFRNGDAVLATSYDIGVAHDGGYA
                     EYARIPADWVLPIPHGLDLFEAMALGTAGFTAALAVERMEHDGLTPASGPVLVTGASG
                     GVGSIAIDILAGAGYEVVALTGKEKQTAYLKSLGAKEVLSRAALDVAKIKPLDKARWA
                     GAVDNLGGDVLSWIASTMQIAGTIASIGLAASPGFNTTVMPFILRGVSLLGI"
     gene            complement(841..1950)
                     /gene="zapE"
                     /locus_tag="VN496_07860"
     CDS             complement(841..1950)
                     /gene="zapE"
                     /locus_tag="VN496_07860"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HXF16904.1"
                     /translation="MSQPAQEDAVQSFAEYARQAARLHGFSLDPAQVRVLKQFQRLYE
                     ELNESERIGYSLLSVFMAKRQIRGLYLSGGVGRGKSFLMDAFFDFVPIARKSRFHFHR
                     FMQDIHHRLRDMQGEENPLEQIAQDIAGEYRLLCLDEFHVTDIGDAMLMRTLLQGLFE
                     EGLVLVTTSNQSPDDLYRDGLQRAQFLPAIELLKQHLELVEVEGGVDYRLRLLEKGGV
                     FHFPVDAAADKAMATSFEAIAGVPGSRNVELDIEGRPIKALRVGPGVAWFAFDELCDG
                     PRGQPDYIELSRRYHTVLISAVRRIGATDADRRRRFTWLVDEFYDRRVKLIMSADTPV
                     SGLLEAAPAEVEVQRTASRLVEMQTHHYLSEPHLA"
     gene            complement(1950..3377)
                     /gene="lpdA"
                     /locus_tag="VN496_07865"
     CDS             complement(1950..3377)
                     /gene="lpdA"
                     /locus_tag="VN496_07865"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXF16905.1"
                     /translation="MPKTFDVAVIGAGPGGYTAAIRAAQLGLNTVCIDDWKNAKGKLS
                     LGGTCLNVGCIPSKALLESSEDYERALHKFGDHGITVKDVAIDVAKMMARKDKIVDTF
                     TGGIGLLFRKNKVVSMHGSGRFAGKAGGYQIDVIDGQSKETIEAKYVIIATGSSARQL
                     PFAPVDNDRIVDNVGALSLPDIPKKLGVIGAGVIGLEMGSVWKRLGSEVTMLEALPGF
                     LPAADEQVAKEAFRVFTKLVGLDIRLGVKIDSVQASKKAVIAKFTDGVGSQTAEFDKV
                     IVAVGRVPNSAQLGAENVGLKIDARGFVEVDEQCRTSLADVYAIGDVVRGPMLAHKSS
                     EEGVAVAEMIAGQSGHINLDTVPWVIYTSPEIAWVGKTEQELKAKAADYRVGQFPFIV
                     NGRARAIGETAGFVKMLADAKTDRILGVHIIGPYASELIAEAVVAMEFGASAEDIARI
                     VHAHPTLSEALHEAALAVDKRTINL"
     gene            complement(3431..4636)
                     /gene="odhB"
                     /locus_tag="VN496_07870"
     CDS             complement(3431..4636)
                     /gene="odhB"
                     /locus_tag="VN496_07870"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXF16906.1"
                     /translation="MLVEVKIPALSESVAEATLLSWHKKTGDFVRRDENLIDIETDKV
                     VLELPAPASGVLSKIIKGDGGTVTSNEIIATIDTDAKASAEGAAVTPAAATAAIPKDM
                     PAAATAQVAVMPAAKKIAADNNLDTSQISGSGRGGRVTKEDVTNLLRTPAPKPPLPSP
                     VNIERILGERPEQRVAMSRLRQRVAQRLVESQSTAAILTTFNEVNMQPVMELRNRYKD
                     KFEKEYGVKLGFMSFFVKAAVHALKKYPVVNASVDGNDIVYHGYFDVGIAVGSPRGLV
                     VPILRNGDQLSFADVEKQITDFGKRAQDGKLSIEELTGGTFSISNGGVFGSMLSTPII
                     NPPQSAILGVHATKDRAVVENGQIVVRPMNYLALSYDHRIIDGREAVLFLVAIKDALE
                     DPSRLLLEL"
     gene            complement(4679..7507)
                     /locus_tag="VN496_07875"
     CDS             complement(4679..7507)
                     /locus_tag="VN496_07875"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXF16907.1"
                     /translation="MKESLGTSYLFGGNAPFIEDLYETYLDDPQAVTPEWRRYFDQMQ
                     QQSGGARDIAHAPVVESFVRLARMRGKADRQVQGAAEGTASVEKKQVSVLQLINAYRF
                     LGVRHANVDPLKRFDKPIVPELDPAYYGLGEADMNLVFNSGSLVGPDQMPLRDILRAV
                     KETYCGSVGIEYMYITDTQIKRWIQQRFESIRSQPTYDPDFKRHVLERLTAAETLEKY
                     LHTRYVGQKRFSLEGSESVIPLLDLALQRAGTRGIQETVIGMAHRGRLNVLVNTLGKM
                     PKDLFSEFEGKQVEQLPMGDVKYHQGFSSDIMTPGGPMHLTVAFNPSHLEIVNPVVEG
                     SVRARQHRRKDRDGTQVLPILLHGDASFIGQGVVMETLNLSQTRGYGTGGTLHIIVNN
                     QIGFTTSDTRDTRSTLYCTDVAKMVEAPIFHVNGDDPEAVALVTETALDFRMEFHKDV
                     VIDLVCFRRLGHNEQDEPMVTQPLMYKLIAQHPGTRKLYAEKLAAQGVIKPADADDLI
                     KTYRDALDAGYHTNKTILSNYKPPFQVNWAPFKNIPWTYKAATGLPMDRLKELATKLT
                     TVPQHFKLHPRVEKIIADRRAMAAGTSPLDWGMAENLAYASLLKDGYAVRLSGQDSGR
                     GTFFHRHAVWHDQNRERWDAGTYIPLQHISESQPDFVVIDSVLSEEAVLGFEYGYATA
                     EPNELVIWEAQFGDFANGAQVVIDQFIAAGEVKWGRICGLALMLPHGYEGQGPEHSSA
                     RIERYLQLCADYNMQICIPSTPAQMFHLLRRQMIRPFRKPLIIISPKSLLRHKESVSS
                     LEDLANGEFQVVIPDADLQHTQKVKRVVFCSGKVYFDLVAARRERGIDDVAIVRLEQL
                     YPFPHDQFKIEIDRYKDAKEIVWCQEEPGNQGAWHRIQHYLQRQMLPHQKLSYALRPS
                     SSTPAGGYLALHNQRQKAVVDAALSHGV"
     gene            complement(7579..8877)
                     /gene="gltA"
                     /locus_tag="VN496_07880"
     CDS             complement(7579..8877)
                     /gene="gltA"
                     /locus_tag="VN496_07880"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HXF16908.1"
                     /translation="MDKERTATISFNDGSTPIDLPIMNGTIGPDVIDIRTLLNKTGKF
                     TYDPGYLSTASCRSAITYIDGDAGVLLYRGYPIEQLAQQCNFLEVCYLLLTGELPNKV
                     QKDQFVTTVTRHTMVHDQLNNFLRGFRRDAHPMAVMVGIVGALSAFYHDSLDLNNPAH
                     RDISAFRLIAKMPTITAMAYKYSMGQPFVYPLNSLSYSANFMRMMFAVPAEEYQVNDV
                     LVRALDRIFILHADHEQNASTSTVRLSGSSGANPFACIAAGIACLWGPAHGGANEAAL
                     NMLEQIGDVSKIGDFITRAKDKNSGVKLMGFGHRVYKNYDPRAKLMRETCHEVLNELG
                     LQNDRLFKLAMALEKIALEDDYFVSRKLYPNVDFYSGIVQRALGIPVSMFTCIFALAR
                     TVGWIAQWNEMIADPEQKIGRPRQLYTGAAKRDVMPIEKR"
     gene            complement(8964..9191)
                     /locus_tag="VN496_07885"
     CDS             complement(8964..9191)
                     /locus_tag="VN496_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HXF16909.1"
                     /translation="MAEADRIRWQCRRGLLELDLLLARFLDRHFENLSPADLATFKRL
                     LEYPDNDLLDLTLRKIPPPDAETAALIKLFE"
     gene            complement(9200..9895)
                     /locus_tag="VN496_07890"
     CDS             complement(9200..9895)
                     /locus_tag="VN496_07890"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HXF16910.1"
                     /translation="MKFRIYRFDPDQDSKPRMQDYDVEMQPSDRMLLDALVRIKCIDD
                     SLSLRRSCREGVCGSDAMNINGKNGLACITRLSDLKEPVELRPLPGLPVIRDLIVDMT
                     QFFKQYHSVKPYVINNEPLPEKERLQSPAEREALDGLYECILCACCSTACPSFWWNPD
                     KFVGPAGLLQAYRFLADSRDEATNERLDNLEDPYRLFRCHSIMNCVDVCPKGLNPTRA
                     IGHIKDLLVKRAV"
     gene            complement(9966..11732)
                     /gene="sdhA"
                     /locus_tag="VN496_07895"
     CDS             complement(9966..11732)
                     /gene="sdhA"
                     /locus_tag="VN496_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HXF16911.1"
                     /translation="MTLPVRKFDAVIVGAGGAGMRASLQLAEAGFKVAVLSKVFPTRS
                     HTVAAQGGIGAALGNMGEDSWMWHMYDTVKGSDWLGDQDAIEFLCREAPKVVIELEHF
                     GMPFDRNANGTVYQRPFGGHMQHFGAGPAVQRSCCAADRTGHAMLHTLYQRNVRSKTH
                     FFVEWMALDLVRDASGQVLGVTAMEMETGEVSIIHARAVLFATGGAGRIYSASTNAFI
                     NTGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGYLLNKNGERFMER
                     YAPNAKDLASRDVVSRAMATEIKEGRGCGPEADYLLLKLDHLGVALIEDKLPGIREIA
                     KKFANVDPVKDPIPVVPTCHYQMGGIPTNYRGEVVAPQAGNAEAVVTGFYAAGECACA
                     SVHGANRLGTNSLTDLLVFGKASGDTIIRFLKENPGHKDLPKDAADGTLARLSRLDGQ
                     QGGENVYEVANDMRRIMQAHCGVFRFPDMLLEGVKKIKEVAQRGQRTQIRDKSRVFNT
                     ARVEALELDNLIEVAIATLVSAEARKESRGAHDRADHPNRDDVNWLKHSLWFKQGSRL
                     DYKPVHLQPLTVQSIEPKVRTY"
     gene            complement(11736..12083)
                     /gene="sdhD"
                     /locus_tag="VN496_07900"
     CDS             complement(11736..12083)
                     /gene="sdhD"
                     /locus_tag="VN496_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HXF16912.1"
                     /translation="MVKRIVTGAHYGLKDWLAQRITALVMAVYVVVFAGMLLVCPVQG
                     HEQWRALFDHQTMRVATLIFFLSLFWHAWVGMRDILMDYAKHTGVRLGLEVIVVLVLA
                     AYALWAVEILWSF"
     gene            complement(12077..12457)
                     /gene="sdhC"
                     /locus_tag="VN496_07905"
     CDS             complement(12077..12457)
                     /gene="sdhC"
                     /locus_tag="VN496_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HXF16913.1"
                     /translation="MMTASQRPKYLNPFRIRLPLPGLVSILHRISGFALFAFSGALLY
                     LLQEVLRSADGYMHFREITGHWLVKLFLIGMLWSFLHHFFAGIRFLLLDVHIAGDLRA
                     ARATSAAVLVVSLALTAIIGVRLW"
     gene            complement(12472..13212)
                     /locus_tag="VN496_07910"
     CDS             complement(12472..13212)
                     /locus_tag="VN496_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXF16914.1"
                     /translation="MKTSANPNAPAFQPLYRQIKALITDSLVSGEWRPGDLIPSEMEL
                     ASRFSVSQGTVRKAIGELAHQNLLVRQQGKGTFVASHSEERSKFPYLRITPDRGALDE
                     VEARLIDCRKIKLDAASGKKLGLASGTSAYLIKRVMMISGKKAIYEEIRLPAMRFKGL
                     SAETIERHHCMLYSMYETQFDVRILHVEEQIKALPAEAAAATKLGVGEKTPLLVIERV
                     AYTYAEKAVELRRSHCNTAEHYYRNHIV"
     gene            13427..14413
                     /locus_tag="VN496_07915"
     CDS             13427..14413
                     /locus_tag="VN496_07915"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXF16915.1"
                     /translation="MKSPLRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQMLEIA
                     DEKAQAALKGVMMELEDCAFPLLAGMTAASDPKVAFRDIDAALLVGARPRSKGMERKD
                     LLEANGAIFTVQGRALDEVAKRSAKILVVGNPANTNAYIAMNSAPGLSRDNFTAMLRL
                     DHNRALSQLAAKSGKPVDSVDKLIVWGNHSTTMYPDYRFATIAGQPAPKVINNESWYR
                     EVFIPTVAKRGAAIIEARGLSSAASAANAAIDHMRDWNMGTNGKWVTMGFASDGSYGI
                     PDGVMYGFPVTCAGGKYQLVKGLEIDPFSRARMDATLKELEEERDAIKHLLK"
     gene            14520..15404
                     /gene="prpB"
                     /locus_tag="VN496_07920"
     CDS             14520..15404
                     /gene="prpB"
                     /locus_tag="VN496_07920"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HXF16916.1"
                     /translation="MQNSSGQKFRAAVATEKPLQVIGAINAYTARMAEATGYRALYLS
                     GGGVAANSLGMPDLGISTLEDVLTDVSRITEVTTLPLLVDIDTGWGSAFNIARTVRAM
                     IKAGAGAVHMEDQVGAKRCGHRPGKEVVSKEEMVDRVKAAVDARTDAGFVIMARTDAL
                     AVEGLEAAIDRACAYVEAGADMIFPEAMTALPMYSTFKRAVKVPILANITEFGQTPLF
                     TTAELGSADVDIVLYCCSAYRAMNAAALRVYQTIRAEGTQKSLIPMMQSRADLYKYLG
                     YHDYEHKLDELFAKGKQQ"
     gene            15401..16555
                     /gene="prpC"
                     /locus_tag="VN496_07925"
     CDS             15401..16555
                     /gene="prpC"
                     /locus_tag="VN496_07925"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HXF16917.1"
                     /translation="MNEPLASTPKPKKSVALSGVTAGNTALCTVGRTGNDLHYCGYDI
                     LDFAESAEFEEIAHLLVHGKLPTVAELAAYKRKLKSLRGLPAVVQDVLECIPANTHPM
                     DVLRTGCSALGAVLPEDEDHKLGQARDIADRMMASFGSMLLYWYHFSHSSRRIDVLTD
                     DDSIGGHFLHLLHGKKPSALWERAMHTSLILYAEHEFNASTFTARVIAGTGSDMYSAI
                     GGAIGALKGPKHGGANEHAFDTIGRYNNPDQAESDIIKRIAEKQIIIGFGHPVYTISD
                     PRNKVIKEVARTLSRDASDMKLYDISERIEQVMWREKKMFPNLDWYSATSYHMIGVPT
                     AMFTPIFVISRTSGWCAHVIEQRQDGKIIRPSANYIGPENRKWVPIDKRS"
     gene            16667..18118
                     /locus_tag="VN496_07930"
     CDS             16667..18118
                     /locus_tag="VN496_07930"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HXF16918.1"
                     /translation="MSAPISNVRPQPDQVLLDIVDYVTQYKIVSDEAYETARYCLIDT
                     LGCGFEALEYPACTKLLGPVVQGTVVPHGAKVPGTLHQLDPIQAAFNIGTMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAIAAGKKPLTVQEVLTAMIKAHEIQGCIAL
                     ENSFNKVGLDHVVLVKVASTAVVAEMLGLSRDEIINAVSLAWVDGQSLRTYRHAPNTG
                     SRKSWAAGDATSRAVRLALIAKTGEMGYPSVLSAKTWGFYDVLFKGAAFKFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMQIHPELKKLGRNPDDIKKITIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAVPILYGHLTASDYEDEVARDPGIDRLRDKITCVEDKDFT
                     RDYYDPEKRSIANALTVEFSDGKKLNEIVVEYPIGHKRRRKEGMPVLVEKFKTNLARR
                     FPAKQQKRILELCLDAKQLAATPVNEFVDMMVI"
     gene            18126..18548
                     /locus_tag="VN496_07935"
     CDS             18126..18548
                     /locus_tag="VN496_07935"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HXF16919.1"
                     /translation="MRNIGLMLVGMLSALPATADTNPMQPGTWEFTRSGGMAANLIGR
                     FCITAQDVADPMSPVNGFLSREDKNTCNPWQVDWHGERAEYAGSCKLGGKPAQVRGRI
                     VAGTGTYSDTQDIKKEGDTTASPILDIINGLRLGPCAN"
     gene            18574..>21151
                     /locus_tag="VN496_07940"
     CDS             18574..>21151
                     /locus_tag="VN496_07940"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXF16920.1"
                     /translation="MAHNLFNTLQEFSPGPGRKGKFYSLPALEKAGVGNISRLPVSIR
                     IVLESVLRNFDGKKITEEHVRQLASWAPNAPRTAEIPFVVARIILQDFTGVPLLADLA
                     AMRGVASRMGKNPKLIEPLVPVDLVVDHSVQIDHYGSKNALDLNMKLEFQRNKERYQF
                     MKWGMQAFDTFKVVPPGVGIVHQVNLEYLARGLHEKNGVHYPDTLVGTDSHTTMINGI
                     GVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGKLREGVTATDLVLTVTELLRQQK
                     VVGKFVEFFGEGTATLAVPDRATIGNMAPEYGATMGFFPVDDATVEYFRGTGRSDKEI
                     AAFAAYFKAQGLYGVPKTGDLDFSKELALDLSSITPSLAGPKRPQDRIELGKLKSTFS
                     SLFARPIAENGYSKAASDLNKRFATRYGELPQTMVGGGEQAQETAPKARSNEHGLVSR
                     KNTSIPTEIEMMDNRPTPDRVAASARDRAHVAIGHGDVLIAAITSCTNTSNPGVLIAA
                     GLLAKKAVEKGLTVKPHIKTSLAPGSRVVTDYLTKGGLLPYLEKLGFYLAGYGCTTCI
                     GNAGPLDPQLEEVVVQNDLIACAVLSGNRNFEARIHANIKANFLASPPLVVAYAIAGT
                     VLKDLMTEPVGVGKNGKPVYLGDIWPTAKEVYDLMRYATDAKTFRRLYGDLTKDQPLW
                     NQIPSASGQVYDWPKSTYIAEPPFFDDFGMQPGAISKIEGARALGIFGDSVTTDHISP
                     AGSIKDTSPAGKYLIANGVLKADFNSYGARRGNHEVMMRGTFANVRIKNLMVPARADG
                     TRVEGGVTIFQPSGELISIYDAAMKYVAQGTPTVIFGGEEYGTGSSRDWAAKGTQLLG
                     VRA"
BASE COUNT         4547 a         6241 c         5937 g         4426 t
ORIGIN      
        1 tcgataccca gcaagcttac gccgcgcagg ataaacggca ttacggtggt gttgaaaccc
       61 gggctggccg ccagaccgat actggcgatt gtgccggcga tttgcatggt actcgcgatc
      121 catgacagga catcgccgcc gagattatct acggcgcccg cccaccgagc cttgtcgagc
      181 ggcttgatct tggccacgtc gagagccgcg cgcgacagaa cttccttcgc accgagactt
      241 ttcaggtaag cggtttgctt ttccttgccg gtcagcgcaa cgacctcata acctgcaccg
      301 gcgagaatat cgattgcgat gctgcctacg ccaccgctcg ctccggtgac gagaaccgga
      361 ccgctcgctg gcgtgagacc gtcgtgctcc atgcgttcaa ccgccagtgc cgcggtaaag
      421 ccggccgttc ccagtgccat tgcttcgaac aggtcaagcc cgtgcggtat cggcagcacc
      481 cagtcggccg gaatgcgtgc gtattcggca taaccgccat catgagcgac gccaatgtca
      541 tagctggtgg cgagcactgc gtcgccgttg cggaatcgga catcggcgga ttcgaccact
      601 gtgccggaca gatcaatgcc gcccacacag ggaaagcggc ggatgatttt gccggcgccc
      661 gtcgcggcaa gtgcgtcctt gaagttaacg ctcgaatacg caactttgat aaccacctcg
      721 cctgcatcga gctcatccag gcgcatgtcg acataacgcc cggcaacctt gctgtcctcg
      781 ctgaaaattc ggtaggcctt caaggtcgtc aatgcacgcg ctcctcggaa ggcgtttatg
      841 ttaagccaga tgcggctcgc tgagatagtg gtgcgtctgc atttccacca accggctggc
      901 ggtgcgttgc acctcgactt ctgccggggc tgcctccagc aacccgctca ccggtgtatc
      961 ggctgacatg atcagtttga cacggcgatc gtaaaattca tccaccagcc aggtaaagcg
     1021 ccgccgccgg tcggcgtctg tcgcgccgat gcgcctcacc gcggaaatca gcacggtatg
     1081 gtagcgccgg gagagttcga tatagtccgg ctgcccgcgt ggaccgtcgc agagttcatc
     1141 gaaagcaaac caggccacgc ccggaccaac acgcaacgcc tttatcgggc ggccttcgat
     1201 atccagttcc acgttgcggc tgcccggtac gccggcaatc gcttcgaagc tggtcgccat
     1261 ggccttgtct gcggccgcat caaccggaaa atggaacacg ccaccttttt cgagtagccg
     1321 taagcggtag tccacgccgc cctcgacttc gaccagttcc aaatgctgct tcagcagttc
     1381 aatggccggc aggaactgcg cgcgctgcaa cccgtcacga tacaaatcat ccggactctg
     1441 gttcgaggtc gttacgagca ccagcccttc ttcgaacaag ccctggagca aagtgcgcat
     1501 cagcatggcg tcgccgatgt cggtgacatg aaattcatct agacacagca accggtattc
     1561 gccagcgatg tcctgcgcga tctgctccag tggattctct tccccctgca tgtctcgcaa
     1621 acgatgatgg atgtcctgca tgaaacgatg gaaatgaaaa cggcttttgc gtgcgatcgg
     1681 cacaaaatcg aaaaacgcgt ccatcagaaa acttttgccc cgtcccacgc ctcccgacag
     1741 gtatagaccc cggatctggc gcttggccat gaacacgctc aatagcgaat agccgattcg
     1801 ttctgattcg ttcagttcct catacagccg ctggaactgt ttgagcacgc ggacctgagc
     1861 ggggtccagc gagaaaccgt gcagccgtgc cgcctggcgg gcatattcgg cgaaggactg
     1921 aactgcatct tcctgcgccg gctggctcat cacaggttga tggtgcgctt gtcaacggca
     1981 agcgcagctt cgtgcagcgc ttcactcagg gtgggatggg cgtgcacgat gcgcgcgata
     2041 tcttctgcag atgcgccaaa ctccatggcc acgaccgctt cagcgatcaa ttccgatgca
     2101 tacggtccaa tgatatgaac tccgaggatg cggtccgtct tcgcatccgc cagcatcttt
     2161 acaaatcccg cagtctcgcc aatggcgcgc gcgcggccgt tcacgatgaa cgggaactgt
     2221 ccgacgcgat aatcggccgc tttggccttg agttcctgct cggtcttgcc cacccaggca
     2281 atttccggag acgtatagat gacccacggg actgtatcga ggttgatgtg tccggactgc
     2341 ccggcgatca tctccgccac tgccacacct tcttcagacg atttgtgcgc aagcatgggc
     2401 ccgcgcacca catccccgat cgcatagaca tccgccaggc tggtgcggca ctgctcgtcc
     2461 acttccacaa aaccgcgcgc gtcgatcttg agtccgacgt tttctgcgcc aagctgtgcc
     2521 gagttcggca cgcgtccaac tgccacgatg actttatcga attccgcagt ctggctgcct
     2581 actccatcgg taaatttggc gatcactgct tttttggagg cctgcacgct gtcgatcttt
     2641 acgcccaggc ggatatccag tccgaccaat ttggtaaaaa cccgaaaagc ttccttggca
     2701 acttgttcgt ccgcggccgg caggaaaccg ggcagcgctt ccagcatggt aacttcggac
     2761 cccaggcgct tccagacact gcccatctcc agcccgatga ctcccgcgcc aatgacgccc
     2821 agcttcttgg gaatgtcagg caatgataag gcgccgacgt tgtcgacgat gcgatcattg
     2881 tctactggcg caaatggcaa ctgccgggca ctggagccgg ttgcgatgat gacgtatttg
     2941 gcttcgatcg tttccttgct ttgtccatct atcacgtcaa tctgatagcc ccccgccttg
     3001 ccagcgaagc gaccgctgcc gtgcatcgat accaccttgt tcttgcggaa cagcagccca
     3061 atgccgccgg taaaggtatc gacaatcttg tccttgcgcg ccatcatttt tgccacatcg
     3121 atggccacat ccttcacggt gatgccgtga tcgccaaact tgtgcagcgc gcgttcgtaa
     3181 tcttccgacg actcgagcaa cgccttggag ggaatgcagc cgacattcag gcacgtgccg
     3241 cccagactta acttgccttt ggcgtttttc cagtcatcga tgcaaaccgt gttcaagcca
     3301 agctgcgccg cgcgtatggc cgccgtgtag ccgcccggtc ccgcaccaat gactgcaacg
     3361 tcgaatgttt ttggcatttt tgtctagtat gagcgccctg ctctgggttg cttcagccgc
     3421 accgacggaa ctaaagctcc agcagcagcc gcgacggatc ttcgagcgcg tccttgattg
     3481 ccaccaggaa caagactgct tcgcggccgt cgataatgcg atgatcgtag gagagtgcca
     3541 ggtaattcat cgggcgaacc acgatctgtc cattttcgac tacggcacgg tccttggttg
     3601 catgcacgcc caggatcgcg ctttgtggtg gattgatgat cggcgtcgac agcatggaac
     3661 caaacacgcc gccattggat atcgagaatg ttccgccggt cagttcttca atcgacagtt
     3721 tgccatcctg agcgcgcttg ccaaaatcgg taatctgttt ctccacgtcc gcaaacgata
     3781 gctgatcccc gttgcgcagg atcggaacca ccaacccgcg tggactaccg actgcaatgc
     3841 ctacgtcgaa gtagccgtga taaacaatgt cattgccatc cactgatgca ttcaccaccg
     3901 gatatttttt cagggcatgc actgcggcct tgacgaaaaa ggacatgaag ccaagcttga
     3961 caccgtattc cttttcgaac ttatccttgt aacggttgcg caattccatg acaggctgca
     4021 tgttgacctc gttgaacgtg gtcagaatgg ccgctgtgga ttgagattca accagccgct
     4081 gcgcaacgcg ctggcgcagg cgcgacatcg ccacccgttg ctcgggtcgc tcgcccagaa
     4141 tgcgctctat attgacggga gacggcagcg gaggcttggg cgccggggta cgcaacaggt
     4201 tggtcacgtc ttccttggtg acgcgaccgc cccggccgct accactgatc tgagatgtgt
     4261 ccagattgtt gtccgccgca attttctttg ccgcaggcat caccgcgact tgcgctgtgg
     4321 ctgcagcggg catgtccttt gggattgcag ccgttgcggc agccggcgtc acggcagccc
     4381 cctccgctga tgccttcgca tcggtgtcga tcgtggcgat tatttcgttg ctggtaacag
     4441 tcccgccgtc acccttgatg atcttgctca gcactcccga cgccggtgcc ggcaactcga
     4501 gcacgacttt gtcagtttcg atgtcaatca ggttttcgtc gcgtctgacg aaatcacccg
     4561 tcttcttgtg ccacgacagc aatgtagcct cggcaaccga ttcagagagc gccgggatct
     4621 tcacttcgac tagcatggct ggttccttgt tgtttcgacg tatcgcctcg aacgaaaatc
     4681 agacgccgtg agaaagggca gcgtccacga ccgccttttg tcgctggttg tgcagggcta
     4741 aatagccacc cgccggtgtc gacgaggagg gccgcagcgc gtaggacaat ttctgatgag
     4801 gcagcatctg gcgctgcaaa taatgttgta tgcggtgcca cgcgccctgg ttaccgggtt
     4861 cctcctggca ccacacaatc tctttcgcgt ccttgtagcg atcgatttca atcttgaact
     4921 ggtcatgcgg aaaaggataa agctgttcca gcctgacaat cgcgacgtcg tcgatgcctc
     4981 gctcgcgccg ggccgccacc agatcgaaat agactttgcc actgcagaac accacccgct
     5041 tcaccttttg cgtgtgctgc aggtccgcgt ctggaatcac gacctggaat tcgccgtttg
     5101 ccagatcttc cagcgaagac accgattctt tatggcgcag caggcttttc ggactgatga
     5161 tgatgagtgg cttgcgaaac ggccggatca tctgccggcg cagcaggtgg aacatttgcg
     5221 caggcgtgga aggaatgcag atctgcatgt tgtaatccgc gcacaattgc agataacgct
     5281 cgatgcgcgc cgaagagtgt tccggaccct gcccttcgta accgtggggc aacatcagcg
     5341 ccaatccgca gatccgcccc catttcacct cgcccgcggc gatgaactga tcaattacga
     5401 cttgcgctcc gttggcgaag tctccgaact gggcttccca tatcaccagc tcgttcggtt
     5461 ccgcggtagc gtaaccgtat tcgaatccga gtaccgcttc ttccgaaagc actgaatcga
     5521 tgacaacgaa atcgggctgg ctttcgctga tgtgctgtag cggaatatag gtgccagcat
     5581 cccagcgctc gcggttctgg tcatgccaca ccgcatggcg atgaaaaaaa gtgccgcgac
     5641 cgctatcctg tccggaaagg cggactgcgt acccatcctt cagcagggac gcgtaagcca
     5701 ggttctccgc catgccccag tcgagcggcg aggtccctgc ggccatggcg cgcctgtccg
     5761 caataatctt ttccacgcgc ggatgcaatt tgaagtgctg cgggactgta gtcagtttag
     5821 tcgccagctc cttcaatctg tccatgggca agccggttgc ggccttgtag gtccatggaa
     5881 tatttttgaa tggcgcccag ttgacctgaa acggtggctt gtagttcgaa agaatggtct
     5941 tgttggtgtg gtatcccgca tccagcgcat cgcggtaagt cttgatcagg tcatcggcat
     6001 ccgccggttt gatgacgccc tgggcggcaa gtttttcggc gtagagcttg cgtgttcctg
     6061 gatgctgggc aatcagcttg tacatcaggg gctgggtcac catcggttca tcctgttcgt
     6121 tgtggcccag ccgacgaaag cacacaagat cgatgacgac atctttgtgg aattccatgc
     6181 ggaaatccaa cgccgtttca gtaaccagcg cgaccgcttc cggatcgtca ccgttgacgt
     6241 gaaatatcgg cgcttcgacc atttttgcca cgtcggtaca gtacagcgtg gagcgtgtat
     6301 cgcgcgtatc cgaggtggtg aagccaatct ggttgttgac gatgatgtgc agcgtgccgc
     6361 cagttccgta gccgcgggtt tgcgaaagat tcagcgtctc catgacgacg ccctgcccga
     6421 tgaacgaggc atctccatgc agcagaatcg gcagcacctg cgtgccatca cgatccttgc
     6481 gccgatgctg gcgcgccctc accgatcctt ccacgaccgg attgacaatc tcaaggtgcg
     6541 atggattaaa cgccacggta aggtgcatcg gtccaccggg cgtcatgatg tcggaagaaa
     6601 agccctggtg atacttgacg tcgcccattg gcaattgttc aacctgcttg ccctcgaatt
     6661 ccgaaaacag gtccttgggc atcttgccga gcgtattgac cagcacattg agacggccgc
     6721 gatgcgccat tccaatcacg gtttcctgga tgccccgggt gcctgcgcgc tggagcgcaa
     6781 ggtcgagcag cggaatgacg ctttcgctgc cctcgaggga gaaacgcttt tggcccacat
     6841 aacgtgtatg cagatacttt tccagcgttt cggcggccgt cagacgctcg agcacatggc
     6901 gcttgaaatc ggggtcataa gtgggctggg agcggatgct ttcgaaccgc tgctggatcc
     6961 agcgtttgat ctgcgtatcc gtgatgtaca tgtattcaat gccaacggat ccgcaatagg
     7021 tttccttgac tgcccgcagg atgtcgcgca gtggcatttg gtccggccct accagcgaac
     7081 cgctgttgaa taccaggttc atgtcggctt cgccaaggcc gtagtaggcc ggatcaagct
     7141 ccggaacaat tggtttgtcg aagcgcttca gcggatcgac gttggcatgc cgcacgccca
     7201 ggaaacgata ggcgttgatc agttgcaaaa ccgaaacctg ctttttttcc acgctcgccg
     7261 tgccctcggc ggcgccttgg acctgtcgat cggctttgcc gcgcatccgc gcaagccgaa
     7321 cgaaggattc cactacaggc gcatgagcaa tgtcgcgtgc gccgcctgac tgctgctgca
     7381 tctggtcgaa gtaacgccgc cattcgggcg tgaccgcctg gggatcatcg aggtaggttt
     7441 catagagatc ctcgatgaaa ggcgcgttgc cgccaaacag gtaagaagtg ccgagagatt
     7501 ctttcatcat gacgctattt cctctggttc agatggcgca gaaatggcgg aaatcaaccc
     7561 cggcgcctgt gtgcgttttc agcgtttctc gatgggcatt acatcgcgct tggccgcgcc
     7621 ggtatacagt tgccggggtc ttccaatctt ctgttccgga tccgcaatca tttcattcca
     7681 ctgcgcaatc catcccacgg tgcgcgcgag cgcgaaaatg caggtaaaca tcgaaaccgg
     7741 tatgccgagc gcacgctgca cgatccccga ataaaaatcg acatttggat acaacttgcg
     7801 gctcacgaag taatcgtctt ccagtgcgat tttctccagt gccattgcga gcttgaacag
     7861 acggtcattc tgcaaaccca gctcgttgag cacttcatgg caggtctcgc gcatgagctt
     7921 tgcacgcgga tcgtagttct tgtagacacg atgcccgaag cccatcagtt tcacgccaga
     7981 attcttgtct ttcgcccggg taatgaagtc gccaatcttg ctgacgtctc cgatctgctc
     8041 cagcatgttc aacgcggctt cgttcgcacc gccgtgcgcc gggccccaga ggcacgctat
     8101 cccggccgcg atgcaggcaa atggattggc gcccgatgag ccagacaggc gcacggtcga
     8161 tgtggacgca ttctgctcgt ggtccgcatg caggatgaaa atgcggtcca gcgcgcgtac
     8221 cagcacgtcg ttgacctggt actcctccgc cggaaccgca aacatcatgc gcatgaaatt
     8281 agccgagtac gacaacgaat tgagcggata cacaaacggc tgtcccatcg agtacttgta
     8341 ggccatcgcc gtaattgttg gcatctttgc aatcaggcgg aaagctgaaa tgtcacggtg
     8401 cgccggatta ttaaggtcca gtgaatcgtg atagaaggcc gacagggcgc ctactatgcc
     8461 gaccatcacg gccatcgggt gtgcgtcgcg tcgaaacccg cgcaaaaagt tgttcaactg
     8521 atcgtgaacc atcgtatggc gggtgaccgt cgtgacgaac tgatccttct gcaccttgtt
     8581 cggcaactcg cctgtcaaca gcagatagca cacttccagg aaattacatt gctgcgccag
     8641 ttgctcaatt ggataaccgc ggtacagcag cacaccggcg tcgccatcga tataggtgat
     8701 tgcagaacgg cagcttgcag tggacaggta tccgggatcg tacgtaaatt tcccggtctt
     8761 attcaggagc gtgcggatat cgatcacgtc cggtccgatg gtgccgttca tgatgggtag
     8821 atcgatggga gtcgaaccgt cgttgaacga gattgttgcg gtgcgctcct tgtccatagt
     8881 ctcgctcgct ttcgaactca ttaaggattg aagtttaaca gtttccggcg attgcaacag
     8941 cgttggacgc agtgcaaaaa cttttattca aataatttga tgagcgcggc cgtttcggca
     9001 tccggaggcg gtatttttcg aagcgtcagg tcgaggagat cgttgtcggg atactctagc
     9061 agccgcttga atgtcgcgag atcggccggc gacaaatttt cgaaatgacg gtcgagaaag
     9121 cgcgccagca acaggtccaa ttccagcagc ccgcgcctgc actgccaccg tatgcggtct
     9181 gcttctgcca cggcgttcgc tagaccgcgc gcttgaccag cagatccttg atatgaccga
     9241 tagctcgcgt cggattgagc cctttcgggc agacgtcgac gcaattcatg atggagtggc
     9301 aacgaaacag ccggtaagga tcctcgagat tatcgagccg ctcgttcgtg gcttcgtcac
     9361 ggctgtcggc aagaaaccgg tacgcctgca acaaaccggc cggccccacg aacttgtcgg
     9421 gattccacca gaatgacgga caggccgtgg agcagcaggc gcacaggatg cattcgtaca
     9481 acccatcgag ggcttcacgc tccgcggggg attgcagccg ttccttttcc ggcaacggct
     9541 cattgttgat cacataaggc ttaacagaat ggtactgctt gaagaattgc gtcatgtcga
     9601 cgatcaagtc acgaatgacc ggcaaccccg gcaatgggcg caattcgacc ggctctttca
     9661 aatcgctcaa gcgcgtaata caggcgagac cattcttgcc attgatgttc atcgcatccg
     9721 aaccgcacac gccttcccgg cacgagcgcc gcaacgaaag tgaatcgtcg atgcatttga
     9781 tgcgcaccag cgcatcgagc agcatgcggt cgctcggttg catttcgacg tcgtaatcct
     9841 gcattctcgg cttggaatcc tggtccggat cgaagcggta aatcctgaat ttcatttctt
     9901 ctcctggcga ggggctccgg atttgaatgt cacgttgaaa ttgcctggcg ggctagccgg
     9961 ctgcttcaat aggtcctgac tttcggctcg atactctgca cggtcagcgg ctgcaggtgc
    10021 acgggcttgt aatcgaggcg gctgccctgc ttgaaccaga gcgaatgctt gagccagttc
    10081 acgtcatcgc gattcggatg atcagctcga tcgtgtgcgc cgcggctttc cttgcgcgct
    10141 tcggccgaca ccagcgtagc gattgcgact tcaatcaggt tgtcgagttc cagcgcttcg
    10201 acacgcgcgg tattgaatac cctgctcttg tcgcggatct gtgtgcgctg cccccgctgc
    10261 gcgacttctt ttattttttt aaccccttcc agcagcatgt ccggaaaccg gaatacgcca
    10321 caatgcgcct gcatgatgcg tcgcatgtca ttggcgacct cgtacacgtt ctcgccacct
    10381 tgctggccat cgagccggga cagccgggct aatgtaccgt cagcggcgtc cttgggcaaa
    10441 tctttatggc cgggattttc cttcaaaaac cggataatcg tatcgcccga cgctttgcca
    10501 aacaccaaca gatcggtcag tgaatttgtg ccgagccggt tggcgccatg cacggatgcg
    10561 cacgcgcact ctccggcagc ataaaacccg gtcacgactg cctcggcgtt tcccgcttgc
    10621 ggcgcaacga cctccccgcg atagttcgtc gggatgccgc ccatctggta gtggcaggtc
    10681 ggcaccaccg gaatagggtc ctttaccgga tccacgttgg cgaatttttt ggcgatttcg
    10741 cggatacccg gcaatttgtc ctcgatcagc gcgacgccga gatgatccag cttcagcagc
    10801 aggtaatccg cttcgggccc gcacccgcgg ccttccttga tttcggttgc cattgcgcgc
    10861 gatacaacat cgcggcttgc aaggtccttg gcgttgggcg cgtagcgttc catgaaacgc
    10921 tcgccatttt tgttcaacaa atatccaccc tcgccgcgca cgccctccgt gatcagcacg
    10981 cctgcgcctg ctacgccggt ggggtggaat tgccagaact ccatatcttc gagcgggatg
    11041 ccggcacgag cggccatgcc gagtccatcg ccggtattga tgaaggcgtt ggtgcttgcg
    11101 gaatagatcc ggccagctcc tccagtggcg aacagcactg cgcgcgcatg aatgatcgaa
    11161 acttcgccgg tctccatttc catggcggtg acgcccagta cctggcccga tgcatcgcgc
    11221 accaggtcga gcgccatcca ttcaacgaaa aaatgcgtct tcgaccgaac attgcgctga
    11281 tacagcgtat gcagcatggc gtggcccgtg cggtccgcgg cacagcaaga acgctgcact
    11341 gccggacccg ctccgaaatg ctgcatatga ccgccgaacg gacgctggta aaccgtgccg
    11401 ttggcgttgc ggtcgaaagg catgccgaaa tgctcgagtt cgatgacgac cttgggtgct
    11461 tcgcggcaca gaaattcgat ggcgtcctga tcgcccagcc agtccgaccc tttcaccgtg
    11521 tcgtacatgt gccacatcca gctgtcctcg cccatattgc ccagcgccgc accgatgccg
    11581 ccttgtgccg caaccgtgtg cgaccttgtg ggaaagacct tcgacagcac ggcgaccttg
    11641 aaccccgctt ccgccagttg cagcgaggca cgcataccag ccccgccggc acctacgatt
    11701 accgcatcaa attttcgcac cggcaaggtc atgtatcaaa agctccagag aatctcaacc
    11761 gcccacaggg cataggccgc caggaccagc acgacgataa cttccagacc gagccgtacg
    11821 ccggtgtgtt tcgcgtaatc catcaggatg tcccgcatgc caacccacgc atgccagaac
    11881 aggctcagga aaaaaatcag ggtcgctacg cgcatcgtct gatggtcgaa taatgcgcgc
    11941 cactgttcat gcccttgcac ggggcaaacc agcagcatgc cggcaaacac aacgacgtat
    12001 acggccatga ccagcgctgt aattcgctgt gcaagccagt ctttgagccc atagtgcgca
    12061 ccggtaacga tccgtttcac cataagcgca ccccgataat cgcagtaagg gcgaggctca
    12121 cgaccagcac cgccgcactg gtggcgcgag cagcccgcag atcgcctgcg atatgcacat
    12181 cgagcaacag gaagcgaata cccgcgaaga aatgatgcag gaaggaccac aacataccga
    12241 tcaggaacag tttgaccagc caatggccgg tgatttcgcg aaagtgcata tagccatcgg
    12301 ccgagcgcag cacttcctgc agcaaatata acagcgcccc gctgaaggcg aacaatgcga
    12361 agccgcttat gcgatgcagg atggatacca atcccggtaa tggcaagcgg atccggaacg
    12421 gattgagata ctttggtcgc tggctggcgg tcatcatggt ttccggtgcg atcaaacgat
    12481 gtgattgcgg taataatgtt cagcggtatt gcagtgactg cgacgcagct cgaccgcctt
    12541 ctccgcgtaa gtataggcaa cccgttcgat gaccagcagc ggcgtttttt cccccacacc
    12601 aagcttggtg gctgcggctg cttcggcggg caaggccttg atctgttctt ccacgtgcag
    12661 tatgcgtaca tcgaactgag tttcgtacat gctgtacagc atgcagtggt gccgctctat
    12721 ggtctcggca gacagtccct tgaagcgcat ggccggcagg cggatttcct cgtagattgc
    12781 cttttttccc gaaatcatca tgacgcgttt gatcagatag gcacttgttc cggatgccag
    12841 gccgagcttt ttccccgacg ctgcatcgag cttgatcttg cggcaatcga tcaacctggc
    12901 ctcgacttcg tccagcgcgc cgcgatccgg cgtaatgcgc agataaggaa atttgctgcg
    12961 ttcttcggaa tgcgaggcga caaacgtgcc cttaccttgc tggcgcacca gcaaattctg
    13021 gtgagcgagc tcgccaatag ctttgcgcac cgttccctgg ctgacgctga agcggctggc
    13081 gagttccatt tcgcttggaa tcaggtcgcc gggccgccac tcgccggaca ccaggctgtc
    13141 cgtgatcaaa gccttgatct gccgatagag cggctggaag gcgggcgcgt ttgggttggc
    13201 actggtcttc atgtcacggc ttgattttta cacatcggcg gccgggccgt ccatcaaaag
    13261 gtatctttta taagatataa gacctgtttg acacctgcgg aattgcttcg ttatgatgtt
    13321 gcccgaaaat tggtttgtgc tgcgccctgt atcgcgctgg caaacaacgt agccgacccg
    13381 aattccacct gaatcgccag ctcaccaaac agcaggagcc ctagccatga aatcgcccct
    13441 ccgagtcgca gtcaccggcg ctgccggcca gatcggctac agcctgctgt ttcgcattgc
    13501 gagcggtgaa atgctaggca aggaccagcc ggtcatcctg caaatgctgg aaatcgccga
    13561 cgagaaagcg caggccgcac tcaagggcgt gatgatggaa ctcgaggact gcgcctttcc
    13621 actgctggca ggcatgacgg ccgcgtccga tccgaaagtt gctttccgcg atatcgatgc
    13681 ggcgctgttg gtcggcgccc gtccgcggtc aaaaggaatg gagcgcaagg atttgctgga
    13741 ggccaatggc gcgattttca ccgtccaggg tcgtgcgctc gacgaggtcg ccaagcgttc
    13801 cgccaagatt ttggtggtcg gcaatcctgc caataccaat gcctatattg caatgaacag
    13861 cgcgcctggc ctgtcacgcg acaatttcac cgccatgctg cgcctggacc ataaccgtgc
    13921 cctgtcacag cttgcggcca aatcgggcaa gcccgtcgat tcggtcgaca aattaatcgt
    13981 ctggggtaac cattcgacga cgatgtatcc cgactaccgt tttgcaacca ttgccggcca
    14041 gcccgccccc aaagtcatca acaatgaaag ctggtatcgg gaagttttca tcccgaccgt
    14101 ggcgaagcgc ggcgctgcga ttattgaagc gcgtggtctt tcgtccgccg cctcggcagc
    14161 caatgccgcc atcgatcata tgcgcgactg gaacatgggc acgaatggca aatgggtcac
    14221 catgggtttc gcctcggatg ggtcatacgg aataccggac ggagtaatgt acggattccc
    14281 ggtgacctgc gcgggtggca agtatcagtt ggtcaaaggc cttgagatcg atcctttcag
    14341 ccgcgccaga atggatgcca ctctcaaaga gctggaagaa gagcgtgacg cgatcaaaca
    14401 tctgctgaag taggccgctt gcgtagcaag ggaacattgg cgtattccaa tggtattccg
    14461 cggtccgaat acaaacgacc ggcgtgccgg caggacatga cccttttgag aggcctcata
    14521 tgcaaaacag ctcaggacag aaattccgtg cagccgtcgc cactgaaaag ccgctgcagg
    14581 tcattggcgc aatcaatgcc tataccgcgc gcatggccga agcaacaggg taccgggcgt
    14641 tgtatctctc tggtggcggc gtagctgcga actctctcgg catgcccgat ctgggcatca
    14701 gcacgcttga ggacgtcctg accgacgtca gccgcataac tgaggtcacg accctgccct
    14761 tgctggttga tattgatacc ggctggggca gtgcctttaa cattgcgcgc actgtccgcg
    14821 cgatgatcaa ggccggtgcc ggcgcagtac acatggaaga tcaggtaggc gcaaaacgct
    14881 gcggacatcg gcccggcaag gaagtcgtca gcaaagagga aatggtggac cgggtaaagg
    14941 ccgcggtcga cgcacgcacg gatgccggat tcgtcatcat ggcgcgcacg gacgcgttgg
    15001 cggttgaagg cctcgaagcc gcgatcgacc gcgcgtgtgc gtacgtggaa gccggcgccg
    15061 atatgatttt cccggaagcc atgactgcgc tgccgatgta cagcacattc aagcgtgccg
    15121 tcaaagtgcc catcctggcc aacataaccg aattcggaca gacaccgctg tttaccactg
    15181 ccgaattggg ctcggcggac gtcgatatcg tgctctactg ctgttcggcc tatcgggcca
    15241 tgaatgcggc ggccctcagg gtatatcaga cgatccgcgc ggaaggcacc cagaagagcc
    15301 tcattccgat gatgcaatcg cgcgccgacc tgtataaata cctggggtat cacgactacg
    15361 agcacaagct ggatgaactg ttcgcaaaag gaaaacaaca atgaacgagc cattggcaag
    15421 cacgccgaag cccaagaaat cggtcgcgct atcgggtgtc acagccggca acactgcact
    15481 gtgcacggta ggccgaaccg gcaacgatct gcactattgc ggctatgaca tcctcgattt
    15541 tgccgaaagc gcggaattcg aggaaattgc gcacctgctg gtgcatggca agcttcccac
    15601 ggtagcggag ctcgcggctt acaagcgcaa gctgaaatca ctgcgcgggc tgccggccgt
    15661 cgtgcaggac gtcctggaat gcattccggc aaacacgcat ccgatggatg tcttgcgaac
    15721 cggatgctcg gctctcggag ccgtcttgcc tgaagacgag gatcacaaac tcgggcaggc
    15781 ccgcgacatc gccgaccgca tgatggcgag ctttggttcg atgctgctgt actggtatca
    15841 cttttcgcac tccagccggc gcatcgatgt gctgaccgat gatgattcca ttggcgggca
    15901 tttcctgcac ctgttgcacg gcaagaagcc gtccgcgctg tgggaacggg ccatgcatac
    15961 ctcgctgatc ctgtatgcag agcatgagtt caacgcctct actttcaccg cccgtgtcat
    16021 cgccggcacc ggctcggaca tgtattcggc catcggcggc gcaattggcg cgctgaaagg
    16081 accgaaacat ggtggcgcca acgagcacgc cttcgacacg attggccgct acaataatcc
    16141 cgaccaggcc gaatccgaca tcatcaaacg catcgctgaa aagcagatca tcatcggctt
    16201 tggccatccc gtgtacacca tcagcgatcc gcgtaacaaa gtcatcaagg aagtcgcgcg
    16261 cacattgtcg cgtgatgcca gcgacatgaa gctgtacgac atttccgagc gcatcgagca
    16321 ggtcatgtgg cgcgaaaaga aaatgtttcc gaatctggac tggtatagcg cgaccagcta
    16381 tcacatgatc ggcgtcccga ctgcgatgtt cacgccgatt tttgtcatca gccgcacctc
    16441 gggctggtgc gcacacgtga tcgaacagcg gcaggacggc aagatcattc gccccagcgc
    16501 aaattacatc ggtccggaaa accgaaaatg ggttcccatc gacaagcgct cttagcggct
    16561 taccaacgga aaagcataga actgactgca acggacggag gtcgcgcggc atccggcgca
    16621 cacctccgtg ctgatcaaaa aagtttcgtc ctgaggattt aaattaatgt ccgcccctat
    16681 ttcaaacgta cgcccgcaac ccgaccaggt tctgctcgac atcgttgact atgtgacgca
    16741 gtacaaaatt gtaagtgacg aggcctacga aacggcgcgc tactgcctca tcgatacgct
    16801 ggggtgcggg ttcgaggcgc tggaatatcc cgcgtgcacc aagttgctcg gcccggtggt
    16861 gcaaggcacg gtggttccac acggtgcaaa ggttcctggc accttgcatc agttggatcc
    16921 gatccaggcg gctttcaata tcggcaccat gatccgctgg ctggatttca acgatacctg
    16981 gcttgccgcg gaatggggcc atccctcgga caatcttggt ggcattcttg ccaccgcgga
    17041 ctggctctcg cgcaatgcga tagccgcggg gaaaaaaccg ctaacggtgc aagaagtatt
    17101 gaccgctatg atcaaggccc atgaaatcca gggctgcatt gcgctggaga atagtttcaa
    17161 caaggttggc ctcgatcacg tggtgctggt gaaggttgcc tccaccgccg tcgttgccga
    17221 aatgctcggc ttgtcccgcg atgagatcat caatgccgta tcgctggcgt gggtcgatgg
    17281 ccaatcgctg cgcacttacc ggcatgcgcc gaacaccggc tcacgcaaga gctgggctgc
    17341 gggtgatgct accagccgcg cggtgcgcct cgcgttgata gcgaagaccg gtgaaatggg
    17401 ttatccgtct gtattgagcg ccaaaacatg gggtttctat gacgtgctgt tcaagggcgc
    17461 cgcgttcaag ttccagcgcc cctacggcag ttatgtcatg gaaaacgtgc tgttcaagat
    17521 ttcctttccg gctgaattcc attcacaaac cgccgtggaa gccgccatgc agatccaccc
    17581 ggaattgaaa aaactgggca ggaatccgga cgacatcaaa aagatcacga tccggaccca
    17641 tgaagcctgc atccgcatca ttgacaagaa gggcccgctc aacaatccag cggaccggga
    17701 tcattgcatc cagtacatgg ttgcagtgcc gatactgtat ggccacctga cggcaagtga
    17761 ttacgaagac gaggtcgccc gcgatccggg cattgaccgg ctgcgcgaca aaataacgtg
    17821 cgtggaagac aaggatttca ccagggacta ttacgatccg gaaaagcgat cgattgccaa
    17881 tgcgctgacg gttgaattct ccgacggcaa gaagctcaat gaaattgtgg tcgaatatcc
    17941 aatcggccac aaacgccggc gcaaggaagg catgccggta ctggtggaaa agttcaagac
    18001 caatctggca cgccgcttcc cggccaagca gcaaaaacga atactggagc tgtgcctgga
    18061 tgccaaacaa ctggcagcaa caccggtgaa cgaattcgta gacatgatgg tcatctgaca
    18121 tctcgatgcg aaatatcggc ctgatgctag tcggaatgct aagcgccctg cccgctacgg
    18181 cggataccaa tcccatgcaa cctggcacgt gggagttcac tcgttccggc ggcatggcgg
    18241 ccaacctgat cggacgcttc tgcatcaccg cgcaagatgt cgcggatcca atgtcgccgg
    18301 tcaacggctt cctgtcgcgc gaggacaaga atacctgcaa tccctggcaa gtcgattggc
    18361 acggggagcg ggcagaatac gccggctcat gcaaactggg tggcaaaccc gcgcaagttc
    18421 gcggacgcat cgtcgccggc accggcacct attccgatac gcaggatatc aagaaggaag
    18481 gcgacacgac ggcatcgccc atactggaca tcatcaatgg cctgcgcctg ggcccctgcg
    18541 ccaattaaga gacattcctc tggagcattg ttcatggcac ataatctgtt caacacactg
    18601 caagagttct ctcccggtcc cggccggaaa ggcaagttct actctctccc cgcgttggaa
    18661 aaggcagggg tcggcaacat ttcgcgtttg ccggtttcca ttcgtattgt gctggagtca
    18721 gtgctgcgca atttcgacgg caaaaaaatt accgaagagc atgtgcgcca gctggcaagc
    18781 tgggcaccga atgcaccacg cacggccgaa attccatttg tcgttgcacg catcatattg
    18841 caggacttca ccggcgtccc cttgctcgct gatctggctg ccatgcgcgg agtggcgtcc
    18901 agaatgggca aaaatccgaa gttgatcgaa ccgctggtgc cggttgacct ggtggtcgac
    18961 cactcggtgc agatcgacca ctacggttca aaaaatgcac tcgacctgaa catgaagctg
    19021 gaattccagc gcaacaagga acgctatcag ttcatgaagt ggggcatgca ggcctttgat
    19081 accttcaagg tcgtgccacc cggcgtgggg atcgtccatc aggttaacct cgaatacctg
    19141 gcgcgtggcc tgcatgaaaa gaacggcgtg cattatcccg acacgctggt cggcacggat
    19201 tcccacacca ccatgatcaa cggtatcggc gtagtcggat ggggggtggg cggcatcgag
    19261 gcagaggccg gaatgctggg acagcccgta tatttcctga caccggatgt ggtcggggtc
    19321 aatctgaccg gcaaactacg cgaaggcgtg actgccaccg atttggtgct gaccgtaacg
    19381 gaactgctgc gccagcaaaa agtagtcggc aaattcgtgg agttttttgg agaaggcacc
    19441 gcgaccttgg cagtccccga cagagcaacc attggcaaca tggcgccgga gtacggcgct
    19501 accatgggat tctttccggt cgacgatgcg actgtggaat atttccgggg aaccggacgc
    19561 agcgacaagg agatcgccgc attcgccgcg tacttcaagg cgcagggttt gtatggcgtg
    19621 ccgaaaacgg gagacctcga tttcagcaag gagcttgcgc tggatctctc atccattaca
    19681 ccgagtctcg ccggcccgaa gcgcccgcaa gacagaatcg aactgggcaa actcaagtcc
    19741 acgttttcga gcctgttcgc gcggccgatc gccgagaacg gctatagcaa agctgcgtct
    19801 gatctgaaca agcgctttgc cacgcgatac ggtgaattac cgcaaacgat ggtcggcggc
    19861 ggtgagcagg cgcaggaaac cgcgcccaag gcccggagca acgaacacgg tctggtgagc
    19921 agaaaaaaca ccagcatacc gaccgagatc gagatgatgg acaaccgtcc cacgccggat
    19981 cgggtagccg cctcggcgcg tgaccgcgca cacgtcgcga tcggacatgg cgacgtattg
    20041 atcgctgcaa ttacgtcctg cacgaacacg tccaatcctg gcgtgttgat cgcggccgga
    20101 ctgcttgcca aaaaagctgt cgaaaaaggg ctcacggtca aaccccatat caagacatcg
    20161 ctcgcgcccg gatcgcgcgt ggtaaccgat tatctgacca aaggcggact gctgccctat
    20221 ctggagaaac tcggtttcta tctagccggt tatggctgca ccacctgcat tggcaatgcc
    20281 ggtccgcttg atcctcaact cgaagaagtg gtcgtgcaga acgacctgat tgcgtgtgcc
    20341 gtactctcgg gtaaccggaa tttcgaggca cgcatccacg ccaacatcaa ggcgaatttc
    20401 ctggcttcac cgccactggt cgtcgcttat gccatcgccg gtaccgtact caaagacctg
    20461 atgacggagc cggtcggcgt cggaaaaaat ggcaagccgg tttatctcgg cgatatctgg
    20521 cccactgcga aagaggtcta cgatctcatg agatatgcaa ccgacgcaaa gacgttccgc
    20581 aggctgtatg gcgatctgac caaggaccag ccgctatgga accagattcc ttccgcatct
    20641 ggacaggtct acgactggcc gaaatccact tacatcgccg aaccgccgtt cttcgatgac
    20701 tttggcatgc aaccgggcgc gatcagcaaa atcgagggtg cgcgagcact gggcatattt
    20761 ggcgactcag tcaccaccga ccatatcagt ccggccggct caatcaagga cacttccccc
    20821 gcgggcaaat atctcatagc gaacggcgtg ttgaaggccg atttcaacag ttacggggcg
    20881 cgccggggca atcatgaagt catgatgcgc ggtactttcg ccaacgtgcg gatcaagaat
    20941 ctcatggttc cagccagggc agacggcact cgcgtcgagg gcggtgtgac gatattccag
    21001 ccttccggcg aactgatctc gatctatgac gccgccatga agtacgttgc gcaaggcaca
    21061 ccgaccgtga tattcggcgg agaggaatac ggtaccggat catcacgcga ctgggctgcc
    21121 aaaggaaccc aattgctggg tgtcagggct g
//
LOCUS       DATMZQ010000242         5352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3293707, whole genome shotgun sequence.
ACCESSION   DATMZQ010000242 DATMZQ010000000
VERSION     DATMZQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3293707"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VN496_07945"
     CDS             complement(<1..642)
                     /locus_tag="VN496_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16921.1"
                     /translation="MRRLTIFLRRTFVSITVATLFAVCAGTVAAADEQSNSADPGTAS
                     SPLQTDGSQLRNDDASSPWPLEVKDNGRTFLIYQPEIDKWENNRLDGRSAVSVRSDAD
                     GPPNFGMIYFSARTDSDGNNRSLLVHDALVSKADFPAVAGDDYLAVLRSRFGAHSWRI
                     AQDRLQSDMEIDQLARQSEQQPVRNDPPRILFSERPAVLVPIDGHPVTRPVADT"
     gene            complement(831..1565)
                     /locus_tag="VN496_07950"
     CDS             complement(831..1565)
                     /locus_tag="VN496_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXF16922.1"
                     /translation="MRSLAFLVLFLLSSLVVAADVQSVEVHQELVLPAGTSEKVIALT
                     LDACSGSFDLELTQFLIGHRIHTTIFATRRWIRRNPEALVILNAHADLFEIEDHGANH
                     VPAIIGRDREVYGLAGVADLKHLRSEVSLGAEAVKSATGTAPRWYRGATGEYDPEALK
                     EIEKMGYKVAGFSINADSGATLSQKRVVGRLKKVRSGDIIIAHLNKPQSETGKGLAEG
                     LQMLLDRGFHFVRLDHSVVRPLQKRI"
     gene            complement(1604..2845)
                     /locus_tag="VN496_07955"
     CDS             complement(1604..2845)
                     /locus_tag="VN496_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HXF16923.1"
                     /translation="MQTTILVVFGVVYLGMILGSLPFLQLDRTGIALLGAIALIGAEA
                     LSLEEAWQAVHVPTLILLFAFMVVSAQLRLGGFYTWVTHRTGHLSLSPPKLLAALIGI
                     VAALSAVFSNDVVCIAMAPVLIDVCLARRLNPVPFLLALACSANVGSALTLIGNPQNM
                     LIGERLHLSFSGYLLEAAIPVLLGLLVVWGLIAWRSRGIWALAAEESATDAVPAHLEP
                     APRIDRWQTAKGLSVATVVFVLFLVAPLPREVVALTAAGVLMMSRKLHSRKMLGLVDW
                     QLLVLFMGLFVVNHALEKTGLMHQAVERMAAFGMDLHEPLPLFIASFVLSNIVSNVPA
                     VMLLLPVATHHLAGPLLALASTLAGNLLIVGSIANIIVVDAARRRGLVIDWRRHAGTG
                     IPVTLATMALSWGYLWLRVLH"
     gene            2971..3801
                     /locus_tag="VN496_07960"
     CDS             2971..3801
                     /locus_tag="VN496_07960"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXF16924.1"
                     /translation="MLGVTHIAQYKQLGYVFPVPALTPDEALAVRTSIEDFERQSGLV
                     AGHVIRNKGHLKLTRLYELIFHPNVLDAVESVLGPDILCWGSSLFVKEGNDPGFIAWH
                     QDSYYWGLEPDDVVSAWIALYPSTVENGAMRVIPGSQTMPALAHRKSATQSANMLFTH
                     EELAENVDESQAVDITLAQGDMSLHHIKILHGSPPNRSSARRMGYAIRYVAPHVKQRG
                     DMNSATLVRGKDSYGYFAKDPVPTRDMDPDIVAFVDRAHGSTPVGATSVPASAASIRR
                     "
     gene            complement(3804..4223)
                     /locus_tag="VN496_07965"
     CDS             complement(3804..4223)
                     /locus_tag="VN496_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16925.1"
                     /translation="MHSLAESQWTRVRTILAVMLLLLGCVAAPAGAEQVKIIAPLQND
                     TVHNNEGIMRVDVEVIPPLDKQSGKAVRILLDGKPAAPELPANSFTLEGVERGQHWLQ
                     AVLVDEQGQALQVSDTIYFTMWQASVNNPALNRRPPR"
     gene            4319..4924
                     /locus_tag="VN496_07970"
     CDS             4319..4924
                     /locus_tag="VN496_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16926.1"
                     /translation="MIKASQPTTSVPASSQGLLLIVLGLLFGPGYYAFCQHVSGRAGQ
                     SYSMTERSDRWTLPDGSILRLRGGHAYKPLPLDLTPDQNAYRFRFSFNVTQPDSTDAT
                     NEYQMSLLQGDTGVAERSIKVRGRGKISVVLDPLQIFYPGSYLLVLEEVGTPALAVSG
                     VGLQIDTGVERPKMWIAWSGLVLLVCGIALLLRDALARRRH"
     gene            4941..>5352
                     /locus_tag="VN496_07975"
     CDS             4941..>5352
                     /locus_tag="VN496_07975"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HXF16927.1"
                     /translation="MSWLVIGLALFFGVHLFTSQRPARARLITTLGEGPYKGFYSLVS
                     LAGFGLIVAGMGKAPFIELWDPPGWGRHVTIWLMPVALILLAAAYIPGNIKRFTAHPM
                     LWSFTLWALVHLLANGDVAGLLLFGSFGIYSLYAM"
BASE COUNT         1132 a         1557 c         1563 g         1100 t
ORIGIN      
        1 ggtatccgcg acgggccgcg taaccggatg gccgtcgatc ggaaccagta ctgcagggcg
       61 ttcgctgaac agaatgcggg gcggatcgtt ccggaccggt tgctgctccg attgccgggc
      121 caactggtcg atctccatat ccgattgcag tctgtcctgc gcgatgcgcc aggagtgcgc
      181 tccaaagcgc gaacgcaaca cggcgagata atcatcgccc gcgacggcag gaaagtctgc
      241 cttgctaacc aaagcatcat gaacgagcag tgaacggttg tttccgtcgg aatcggtacg
      301 agcagagaaa tagatcatcc cgaaattagg cggcccatcc gcatcgctgc gtaccgagac
      361 tgcagagcgg ccatcgagac gattgttttc ccatttatcg atttcgggct gatagatcag
      421 aaatgtgcga ccgttatcct tgacctccag cggccagggc gatgaggcgt catcgttccg
      481 cagctgagag ccatcggtct gcagcggtga agacgccgtc cccggatcag cgctgttcga
      541 ctgttcgtct gccgccgcca cggttccagc gcaaacagcg aataacgtgg caaccgttat
      601 ggaaacgaat gttctgcgaa gaaatatagt aagtctgcgc atgggcagct cctcatttgt
      661 gcgacgcacg caaaatggtc ggcgtcacga aatccaacgg gagttatgac ttgcgcgttg
      721 acgcggggtt gcagcggatt tgtcacgcat cgtaacgcga cgcgcggggc acattgcgcg
      781 cggcgccgcg acggcgtcag gccgttatga agcaaggaac ccgaacgagc ttaaatgcgt
      841 ttttgcagcg gtcgtaccac ggagtggtcg agccggacga aatggaaacc gcgatctagc
      901 aacatctgca ggccttctgc aaggcccttg cctgtttccg attgcggctt gttcagatga
      961 gcaatgatga tatctccgct gcggaccttc ttcagtcgcc cgacaacccg tttttgcgac
     1021 aacgtcgctc cgctgtcggc attgatcgag aacccggcca ccttgtatcc cattttttcg
     1081 atttccttca gcgcctcggg atcatattca ccggtcgccc ctctgtacca gcgcggcgca
     1141 gtaccggtag ccgacttcac tgcttcagca ccgagcgata cttcacttct gagatgcttc
     1201 aggtccgcaa cccccgccag tccgtagacc tccctgtctc tgccaatgat agccgggacg
     1261 tgatttgcgc cgtgatcctc tatctcaaat aaatcggcgt gcgcattcag aatgaccagt
     1321 gcctcgggat tgcgtctgat ccagcgccgc gttgcaaaaa tggttgtgtg aatgcggtgc
     1381 ccgatcagga actgggtcag ctcgaggtca aagctgccgc tgcaggcgtc caaagtcagg
     1441 gcgataactt tttcgctggt gccggcgggc aaaacgagtt cctggtgcac ttccacggat
     1501 tgcacatcgg ctgccacaac gagggacgac agcaggaaca ggaccagaaa tgcgagggat
     1561 ctcatgaagc tattaacgca cgcagcagca atcggttgca tgcttaatgt aatacccgta
     1621 gccaaagata tccccaggaa agggccatgg tagccagggt aaccgggatg ccggtgccgg
     1681 catggcgcct ccagtcgatg accaatcctc gccggcgagc cgcatcgacg acgatgatat
     1741 tggcgatact gccgacaatc agcagatttc ccgccagcgt gctggcgagc gccagcaaag
     1801 gtccggccag atggtgggtc gccaccggca gcaacagcat gaccgctgga acgttggaga
     1861 cgatattgga caggacgaag cttgcgataa atagtggtaa tggttcgtgc aaatccatgc
     1921 cgaacgcggc catacgctcg acggcttgat gcatgagacc ggttttttcc agtgcatggt
     1981 tcacgacgaa cagtcccatg aacagcacca atagttgcca gtccaccagg ccaagcattt
     2041 ttctcgaatg cagtttgcgg ctcatcatca agacgcccgc tgcagtgagg gcgacgactt
     2101 cgcgcggcaa gggcgccacg agaaacagga caaacaccac cgtcgcaacc gataagcctt
     2161 tggcagtttg ccaacgatca atccgagggg ccggctccag atgagcaggc acggcatcgg
     2221 tcgcggattc ttccgcggcc agggcccaga ttcctctgct gcgccaggct atcaatcccc
     2281 ataccaccag caatccgagc agtaccggga tggccgcttc cagcagatag cccgaaaacg
     2341 aaaggtgcag ccgctcgccg atcagcatgt tctgcgggtt gccgatgagc gtcagcgccg
     2401 aaccgacatt ggcactgcac gccagcgcca acagaaacgg gaccggattg agccgccgcg
     2461 ccagacacac atcgatcagc actggcgcca tcgcaatgca aaccacgtcg ttgctgaata
     2521 ccgctgacag cgccgcaacg atgccgatga gcgccgcgag caattttggc ggcgatagcg
     2581 acaaatggcc ggtccgatgc gtgacccagg tatagaaccc gcccaggcgc agctgcgcgg
     2641 acacaaccat gaaagcgaac agcaggatca gtgtcggaac atgaacggcc tgccaggcct
     2701 cttccaggct cagggcttca gcgcctatca aggcaatcgc gccgagcagt gcgataccgg
     2761 tgcgatccaa ttgcaaaaag ggcagcgagc ccaggatcat gcccaggtag accacgccaa
     2821 agaccaccag tattgttgtt tgcatgttcc accgcgtctt gcgcgcgtca ctttgcgcgg
     2881 ccgccgcgat ggtatgcgac gccggccgga ttgcctagcg tctcaacaat gcgagaatag
     2941 cgcttcgagg aggaggaacg attgaagaac gtgctcggcg ttacccacat cgcgcaatat
     3001 aaacagttgg gctatgtctt tcccgttccg gcgctgacac ctgacgaagc actcgcggtg
     3061 cgcacgagca ttgaggattt cgagcgccag agcggactcg ttgcagggca tgtgatccgc
     3121 aacaaggggc acctcaaact aacgcggctt tatgaactga ttttccaccc gaacgtgctc
     3181 gatgccgtgg aatcggtgct ggggccggac attctttgtt ggggatcgag cctgttcgtc
     3241 aaagagggca acgatccggg cttcattgcc tggcaccagg attcatatta ctggggcctg
     3301 gaaccggacg atgtggtatc ggcgtggatt gcgttgtatc ccagcacggt agagaacggc
     3361 gccatgcgcg tcatcccggg cagccagacc atgccggcgc tggcccaccg caaatcggcc
     3421 acgcaaagtg caaacatgct gttcacgcat gaagagctcg ccgagaatgt ggacgagtcg
     3481 caggccgtgg atatcacgct tgcacagggc gacatgtcgc ttcatcacat caagattctg
     3541 cacggctcgc cgcccaaccg gtccagcgca cgccgcatgg gctacgcgat ccgctatgtc
     3601 gcgccgcacg taaagcagcg cggcgacatg aactccgcta ctttggtgcg cggtaaagac
     3661 agctacggat atttcgcaaa ggacccggta ccaacgcgcg acatggatcc ggatatcgtc
     3721 gcctttgtag atcgggccca cgggagcacg cccgttggcg ccacatctgt cccggccagc
     3781 gctgccagca tcaggcgctg atcctagcgg ggaggtctgc gattgagcgc gggattgtta
     3841 accgacgctt gccacatcgt gaagtagatc gtgtcggaca cctggagcgc ttgtccttgc
     3901 tcatcgacca gcactgcctg caaccagtgt tgaccacgtt cgacaccttc cagcgtgaag
     3961 ctgttggcgg gcaattcagg cgcggccggt ttaccgtcaa gcagaatacg aaccgccttg
     4021 ccggactgtt tgtcgagcgg cggtatgact tcgacgtcga ctcgcatgat gccctcattg
     4081 ttgtggaccg tatcgttttg cagcggagca atgattttga cttgctccgc cccggccggg
     4141 gcggcgacgc agccaagcaa caacagcatg acggcgagta tcgtcctgac tctcgtccat
     4201 tgggattcag ccaagctgtg catgatgtcc cttcctctct atgccggcta ctgcgctatt
     4261 tgaccataat gcagcagaag atttcccatt ccggcatgcc gggcagataa ttcagcacat
     4321 gatcaaagcc agccaaccca caacgtccgt tccagcaagc tcgcaaggtc tgctgttgat
     4381 tgtactgggc ctgctgtttg ggccgggtta ctacgctttt tgccagcatg tcagcggcag
     4441 ggccggccaa agctattcca tgaccgaacg cagtgatcgc tggacccttc cggatggttc
     4501 gatactcaga ttgcgcggtg gccatgctta caagcccctg ccgcttgact tgacgccgga
     4561 ccagaacgcg taccgctttc ggttcagttt caatgtcacg caacctgact caacagatgc
     4621 aacgaacgag tatcagatga gtttgttgca aggcgatacc ggcgtggcag agcgcagcat
     4681 caaggtccgg ggacgcggca agatttccgt ggtgctggat cctctgcaaa ttttctaccc
     4741 tggcagttat ctattggttc tggaggaagt gggtacgccc gcccttgcgg tgtcgggtgt
     4801 cggtcttcaa atcgataccg gcgtggagcg gccaaaaatg tggattgcct ggagtgggct
     4861 ggtgctgctc gtttgcggca tcgctctttt gctgcgcgat gcactagcac gcaggcgaca
     4921 ttgaaaaaca ggagactgct atgtcatggc tggtgatagg tctggccctt ttctttggcg
     4981 ttcatctctt taccagtcag cgccccgccc gcgcgcgcct catcaccact ttgggagaag
     5041 gcccgtacaa gggcttttat tcgctggtct ccctggctgg tttcggactg atcgtggccg
     5101 gaatgggcaa agcgccgttt atcgagttgt gggatccccc gggctggggg cgtcatgtga
     5161 cgatctggct gatgcccgta gctttgatac ttttggccgc ggcgtatatt cccggaaaca
     5221 tcaagcggtt cactgcccat ccgatgctat ggtcgttcac gctgtgggcg ctggtgcatt
     5281 tgctcgccaa tggcgatgtg gcgggcctgt tgctattcgg ctcctttggt atctattcgc
     5341 tgtatgcgat gt
//
LOCUS       DATMZQ010000243         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2603998, whole genome shotgun sequence.
ACCESSION   DATMZQ010000243 DATMZQ010000000
VERSION     DATMZQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2603998"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VN496_07980"
     CDS             <1..580
                     /locus_tag="VN496_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF16928.1"
                     /translation="APGGTIWEQTEKDWEWTLGVNVWGVIHGIRVFVPRMLEQNVEGH
                     VVNTASVAGLLSLPGMAMYCVSKHSVVTLTECLHHDLAEFGAKIRASVLCPAFVPTGI
                     SDSERNRPAGLREEKAKTTVDLQREEQLRHAVKSGRISADQVAELVFRAIETEKFYIL
                     PHQRIKPSIETRMQDILLERGPTNTLNRKPDS"
     gene            complement(624..920)
                     /locus_tag="VN496_07985"
     CDS             complement(624..920)
                     /locus_tag="VN496_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXF16929.1"
                     /translation="MPYAIQTRDKTGSSKVRADNRPAHIEYLTSQQHLLLAAGALIED
                     DGTGGNGGLLIVDTDDRKEAERFIANDPFTKAGLFEKVTVTRWRKAFFNKERLI"
     gene            complement(961..>2109)
                     /locus_tag="VN496_07990"
     CDS             complement(961..>2109)
                     /locus_tag="VN496_07990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXF16930.1"
                     /translation="EVSALQRQGKDIISFCIGQPDFPTPKNVRDAGISAIKDGKHGYT
                     PSAGILELREGAANYLNRTRHIRVHADDVVVGAGAKPFIAYAIVSTTDHGVGDEVIYP
                     VPGFPIYESQIKAMGAVGVPLYLRESRNFSFDPAELEARITQKTKLLILNSPQNPTGG
                     IIPKKDMEAIATILRKHPQVWVFADEIYSQLCYDGEFCSLTQFPGMLERTIICDGASK
                     TWAMTGWRLGFAANKALAPVFTRWVTNTDSCASQISQWAAVEALNGPQEDAKKMRDSF
                     LSRRNLIVGLLNKVPGVVCQNPGGAFYAWPNVTDACKRIGAADSEEFRKRLLNEAGVA
                     VLADIHFGPRVPNEGQHIRFSYAASNEAIEKGIARMSEFIQKNTRKAA"
BASE COUNT          423 a          599 c          619 g          468 t
ORIGIN      
        1 cgcgcctggg gggactatct gggagcagac tgaaaaggat tgggagtgga ccttgggcgt
       61 aaacgtctgg ggtgtaatcc atggcattcg ggtattcgtt ccacgcatgc tcgaacagaa
      121 cgtggaaggc catgtcgtca acacagcatc cgtcgccggc ttgctgtcct tgcccggcat
      181 ggccatgtac tgcgtatcca aacattcagt ggtaacgctc acggagtgcc tgcaccatga
      241 tctggcggag ttcggcgcga aaatcagggc atccgtactc tgccctgcat tcgttccgac
      301 cgggatttcc gattcggaac gcaaccggcc tgccggtctg cgggaagaaa aagcgaagac
      361 gacagtggat ctgcagcgcg aggaacaact gcggcatgcg gtcaagtcgg ggaggatatc
      421 ggcggaccag gtcgccgaac tcgtcttccg ggccattgag acggagaagt tctacattct
      481 cccgcaccag cgaatcaagc cgtccatcga gacgcggatg caagacatcc tgctcgagcg
      541 cggtccgacc aacacgctaa atcgcaaacc ggattcctga agtcgcgcta cgcttgtccg
      601 gttgtcagcg gtgcgatcgc ttgtcagatc aggcgttcct tgttgaagaa ggccttgcgc
      661 cagcgcgtga ccgttacctt ttcgaacaag ccggccttgg taaacggatc attggcgatg
      721 aatcgttcgg cttccttgcg atcatcggta tccacaatga gcaggccgcc gttgccgccg
      781 gtaccgtcat cctcgatcag cgcgccggct gcgagcagga ggtgctgttg gctggtcagg
      841 tattcgatat gcgcaggccg gttgtccgca cgcactttgc tgctgcctgt cttatccctg
      901 gtttgtatcg cgtatggcac ttgagtgctc cttgattttg gttttgctta agggattgtt
      961 ctaagccgcc ttgcgggtat ttttttggat gaactccgac atgcgcgcga tacccttttc
     1021 aattgcctca ttcgacgcag cgtagctgaa gcggatgtgc tgcccttcat tcggaacgcg
     1081 cggtccgaaa tggatgtccg cgagcacagc gacaccggcc tcgttgagca ggcgcttgcg
     1141 gaactcctcc gaatcggcag cgccgatgcg tttgcaggca tcagtgacat tcggccacgc
     1201 ataaaaggcg cctccaggat tctggcacac tactccgggc accttgttga gcagtcccac
     1261 aataagattg cggcgcgaca ggaaactgtc gcgcattttt tttgcatctt cctgcggccc
     1321 attcagcgct tcgacggcgg cccactggga gatttgtgac gcacaggagt cggtgttggt
     1381 gacccagcgc gtgaatactg gcgccagcgc cttgtttgct gcgaagccaa gccgccaacc
     1441 ggtcattgcc cacgtcttcg atgcaccatc gcaaatgatg gtgcgttcca gcatgccagg
     1501 gaactgagtc agcgaacaaa attctccgtc gtaacacaac tgcgagtaga tctcgtcagc
     1561 gaacacccag acctgcggat gcttgcgcag aatcgtagcg atcgcttcca tgtccttctt
     1621 cgggatgatg ccgccggtcg ggttctgcgg tgaattcaga atcagcagct tggtcttttg
     1681 cgtgatcctg gcctccagtt ccgcaggatc gaaactgaag ttgcgcgatt cacgcaaata
     1741 aagcggcacg ccgaccgcac ccatggcctt gatctgggat tcgtagatcg ggaaacccgg
     1801 gacaggatag atgacttcgt cgccaacgcc atggtcagtg gtcgaaacaa tcgcgtaggc
     1861 gataaagggt tttgcgccgg cgccgaccac gacgtcgtcc gcgtgaacac ggatgtgccg
     1921 agtgcggttc aggtaatttg ccgcgccttc gcgcagttca agaattccag cggacggcgt
     1981 atagccgtgc ttgccatcct tgatggcgct gatgcccgcg tcgcgaacat tcttcggtgt
     2041 cgggaaatcc ggctgcccga tgcaaaatga aatgatgtcc ttgccctggc gctgcaaggc
     2101 actgacttc
//
LOCUS       DATMZQ010000244         5400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1478168, whole genome shotgun sequence.
ACCESSION   DATMZQ010000244 DATMZQ010000000
VERSION     DATMZQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478168"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VN496_07995"
     CDS             complement(<1..868)
                     /locus_tag="VN496_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXF16931.1"
                     /translation="MKFDDFPAQALMEITDRPPLVFVRGEGSWLWDHEGKRYLDFVQG
                     WAVNCLGHSPALITDALVEQSRKLITPSPAFYNEPALRLARMIVEASCFQKVFFTNSG
                     AEANEGAIKLARKWGAMNRNGAYEIITLENSFHGRTLATMSASGKAQFRSLFEPKVPG
                     FPKAKINDIDSVKALITDRTVAVMLEPIQGETGVIIATDAFMRDLRELTEQCKLLLIV
                     DEIQTGVGRTGKLWGYEYSGIRPDIMTLGKGLGGGVPLAALVATEAVSVFEHGDQGGT
                     FNGNPLMAAVGCA"
     gene            complement(1102..2421)
                     /locus_tag="VN496_08000"
     CDS             complement(1102..2421)
                     /locus_tag="VN496_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXF16932.1"
                     /translation="MTAVVLTAGSAAAILFGVFFLLLLLRVPVAIALGLACLPILLIE
                     PRLSPMTLFNETFKAYNSFILLAVPFFLLTANLMNIGGITDRLVRLSRTMVGHFPGSL
                     AQINVVVSVFFAGISGSSTADAASQGKIFIDAQVKEGYGLSFSIAITAVSAVLAVIIP
                     PSILMVVWGGVISTSIGALYLAGILPGLLIAGAQMATVHAYAKIYNYPVYARATLKDF
                     LIAGAQSLPALFTPFIIVGGILLGWFTPTESAAVAVLYAAFLSFFVYREMDLKKLYRA
                     LAETGRLSAVALFCVGTASVFGWLLAYYQIPRVLLENVTSWGLGITGVGFFIAFAFLV
                     VGCFLDAIPAIIIVGTTLQPLAQSVHMHPVQFAIIGIVSLAFGLVTPPYGLCLMISCA
                     IAKVPLKFALKDTLIMLIPMLVVLASCIVWPDIVLILPKLISPEYLK"
     gene            complement(2418..2900)
                     /locus_tag="VN496_08005"
     CDS             complement(2418..2900)
                     /locus_tag="VN496_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXF16933.1"
                     /translation="MKRLLALYHRLLVWLLVGTVAILIVPVTLQILSRFTGLIPSYIW
                     TEELARFMFIWMVMIGAMIGVREGTHFEVDLLPRLGIRANALLRMVSQAFVLIFALVF
                     VWWGIQFTRFGWDQLSEIAELPMWTIFVAWPVVGITSVLFIAEAFVTNVRVLRTGAAQ
                     "
     gene            complement(2897..3895)
                     /locus_tag="VN496_08010"
     CDS             complement(2897..3895)
                     /locus_tag="VN496_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXF16934.1"
                     /translation="MTFTKLLGGIALSAVSALGLLQPAVAQEPITLHGAVQFNDEHVF
                     TRTLFKFEELTKKYYGKPVNFVLHRNSELGLEKDYFVYMNQGKGVDYAIVSPGHMSTF
                     SRAAPFIDAPFLFRDLDHWRKVLDADLFKPIADEVAQKADVLLVGYAGGGTRNIFSNK
                     PVHDLAGLKGLKIRVIGAPIWTRTFAAAGMSPSVIAYNEIYNAIQNGVISAGDNEAAG
                     VEQMRFYEVGPNLVMTQHAITIRPLCFSGKTFKTLPADLQAAILKAGKEAGAYGRQLE
                     SHEDSAKLDAMEKAGKLKRIPFPDRAKMKQMVEPVIEAYAKDVGAEQILGKINAIK"
     gene            complement(4035..4367)
                     /locus_tag="VN496_08015"
     CDS             complement(4035..4367)
                     /locus_tag="VN496_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16935.1"
                     /translation="MAAWLPALKALLPYATQIVTAAIPAFTRKTGTAQDVIPEQIQEL
                     QSAVVHNAESVKILATQMQQVITGIDSGAARIERDMRILRRLSVIATVLSLIAIASCI
                     GLWVVSTG"
     gene            complement(4534..5208)
                     /gene="can"
                     /locus_tag="VN496_08020"
     CDS             complement(4534..5208)
                     /gene="can"
                     /locus_tag="VN496_08020"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HXF16936.1"
                     /translation="MKRLDKLLSNNRLWAASIRSREPDFFTRLSQLQVPEYLWIGCSD
                     SRVPANQITGLAPGEVFVHRNVANIVVHADLNCLSVLQYAVDVLKVKHVIVCGHYGCG
                     GVTAAHDNLKLGLIDNWLRHVQDVAQKHRDALGAIDSREHRIGRLCELNVIEQAVNVA
                     QTTVVQDAWARGQELTVHGWVYDLNDGVIRDLKMSMEGPTDLEDCYVMAMAVADAKTA
                     ALDRSA"
BASE COUNT         1241 a         1587 c         1509 g         1063 t
ORIGIN      
        1 tggcgcagcc gaccgcggcc ataaggggat tgccattgaa cgttccgccc tggtctccat
       61 gctcgaacac cgagactgct tcggtggcga ccagagcagc cagcggcacc ccgccaccga
      121 ggcccttgcc aagggtcatg atgtcgggcc ggatgccgga atattcgtag ccccagagtt
      181 tgcccgtgcg ccctaccccg gtctgtatct cgtccactat cagcaggagc ttgcattgct
      241 cggtgagttc acgcaaatcg cgcatgaaag catcggtcgc gataatcaca ccggtttcgc
      301 cctggatcgg ttccagcatg acggcgaccg tgcgatcggt aatgagtgcc ttcaccgaat
      361 cgatatcgtt gattttggct ttcggaaaac cgggcacttt gggttcgaac aaacttcgaa
      421 actgcgcctt acccgacgcc gacatggtcg caagcgtgcg cccgtggaac gaattctcca
      481 gcgtgatgat ttcgtaggcg ccgttgcgat tcatggcgcc ccacttgcgc gcgagtttga
      541 tggcgccctc attggcttca gcgccgctat tggtaaagaa aaccttctgg aagcaactcg
      601 cctccacaat catgcgggcc agccgcaggg cgggttcgtt atagaaggcc gggcttggcg
      661 taataagctt gcgcgactgc tccaccagcg catccgttat gagagcggga ctgtgaccca
      721 gacagttcac cgcccagccc tgcacaaaat cgagatagcg tttgccttcg tgatcccaca
      781 gccatgatcc ttcaccgcgt acgaaaacca ggggcggccg gtctgtgatc tccatgaggg
      841 cctgcgcggg aaaatcatcg aatttcatgt ttcgatcctc gtattgaata cagtctgaaa
      901 aacaaaaagc cgcaaatgtc tgcggccttc tgggggatgt ccggttgatc gaaacctatt
      961 ttcgtcggcc gcaggcacgc ttcgcgcagg ttgcgcagcg gcgtcgaaca taatgcagga
     1021 accggtattt catgccgcta ttgtcggttg ccggcgccgg cggcgtcaag cccggcagtt
     1081 cagaccctgg cactaaagac attacttcag gtattcgggg gagatcagct tcggcagaat
     1141 cagcacgata tccggccaga cgatgcagga agccagcacg accagcattg ggatcagcat
     1201 gatcagcgta tccttcagcg cgaacttcag cgggaccttc gcgatcgcgc atgaaatcat
     1261 caggcaaagc ccatacggcg gcgttaccag accgaacgcc agtgagacga tgccaatgat
     1321 ggcgaactgc accggatgca tgtgtactga ctgtgcaagc ggctgcaacg tggtgccgac
     1381 gatgatgatc gcaggaatgg cgtcgagaaa gcagcccacc accaggaacg cgaaggcgat
     1441 gaagaatccg acgccggtaa tccccagtcc ccaggacgtc acgttttcca gaagcacgcg
     1501 cggaatctgg taatacgcaa gcaaccagcc gaacactgag gcggtgccga cgcagaacag
     1561 tgccaccgca gacaacctgc cggtctcggc cagtgcgcgg tatagctttt ttaggtccat
     1621 ctcgcggtac acgaaaaagg acaaaaaagc ggcatagagc acggcgacgg cggcggactc
     1681 agtcggggtg aaccagccga gcaggatgcc gccaacaatg atgaacgggg taaacagggc
     1741 cggcaaagat tgcgcacccg ctatcaagaa atccttcaac gtggcgcgcg cataaactgg
     1801 atagttataa atcttggcgt aggcgtgtac ggtcgccatc tgcgcgccgg cgatcagcag
     1861 ccccggcaga atgcccgcca gatacagagc gccgatggat gtggaaataa caccgcccca
     1921 gaccaccatg agaatcgaag gcgggataat gacggcaagc actgcggaca cggccgtgat
     1981 cgcaatcgaa aacgacaggc cataaccctc cttgacctgt gcatcgatga agatcttgcc
     2041 ctggctcgcc gcatccgcgg tcgaggatcc cgaaatcccc gcgaaaaaca cagagacgac
     2101 gacgttgatc tgcgcgaggg aacccgggaa atggccgacc atggtgcgcg acagccggac
     2161 taatcgatcc gtgataccgc cgatgttcat caaatttgcg gtcagcagga aaaaaggcac
     2221 ggcgagcaga atgaacgagt tgtaggcctt gaaggtttcg ttgaagagcg tcatggggga
     2281 gagccgcggc tcgatcagca agatcggcag gcaggcaagc ccgagcgcaa tggcgaccgg
     2341 cacacgcagc agcagcaaca aaaaaaacac gccgaacagg attgcggcag ccgagcctgc
     2401 cgtcagcacg acggcggtca ttgcgccgcg ccggtgcgca aaacgcgcac atttgtgacg
     2461 aaagcctcag cgatgaacaa caccgaggtg atgccaacga ccggccatgc cacaaaaatg
     2521 gtccacatcg gcagttctgc aatttcggac agctggtccc agccgaagcg cgtgaattgg
     2581 atgccccacc agacgaacac caacgcaaaa atcaatacga aagcttggga gaccatgcgc
     2641 aatagcgcat tggcccttat gccgagccgt ggcaagagat caacctcgaa atgcgtgcct
     2701 tcgcgcacac cgatcatcgc gccgatcatg accatccaga tgaacatgaa gcgcgcaagt
     2761 tcctccgtcc agatatacga tggaatcaga ccggtaaagc gtgacaggat ctgaagcgtg
     2821 acgggcacga tcaaaatcgc aaccgtgccg accagcagcc agaccagcag ccggtgatag
     2881 agcgcgagca gacgcttcat ttgatggcgt tgatcttgcc gaggatctgt tctgctccga
     2941 catcctttgc ataggcttcg atgaccggct ccaccatctg cttcattttt gcccggtccg
     3001 gaaatggaat gcgtttcagc ttgccggctt tctccattgc atcgagtttt gcagaatcct
     3061 catgcgattc aagctggcgt ccataggcgc ccgcctcctt gcccgccttc aggatcgcgg
     3121 cctgcaggtc agccggcaaa gtcttgaacg tcttgccgga aaagcacagc ggccgaatgg
     3181 tgatggcgtg ctgtgtcatg acaagattgg gtcccacctc gtagaatctc atttgctcaa
     3241 ccccggccgc ctcgttgtca cccgcagaga taacgccgtt ctggattgcg ttatagatct
     3301 cgttgtaggc aatcaccgag ggcgacatgc cagcggctgc gaacgttcgc gtccagatcg
     3361 gcgcgccgat aacgcgaatc ttgaggcctt tgagtcccgc caggtcatgc actggcttgt
     3421 ttgaaaaaat atttcgtgtg ccgccgccag cataaccgac gagcaggaca tcggcttttt
     3481 gcgcaacttc atccgcgatg ggtttgaaca agtccgcgtc gagcactttg cgccagtggt
     3541 ccagatcgcg gaacaggaac ggagcatcga taaacggtgc cgcgcgcgag aacgtggaca
     3601 tatgcccggg tgacacgatc gcatagtcca ctcccttgcc ctggttcatg tatacgaagt
     3661 agtctttctc caggccaagt tcgctattgc ggtgaagaac gaaattgact ggcttgccgt
     3721 aatatttttt ggtcagttct tcaaacttga acagcgtccg ggtaaacaca tgctcgtcgt
     3781 tgaactgaac cgccccgtgc aacgtgatgg gttcctgtgc caccgccggc tgtagcaagc
     3841 ccagcgcgga caccgcggat agcgcaatgc caccgagcaa ttttgtgaat gtcatgcttt
     3901 ctccctatcg tatgatgcag tgcgaccttg acggcccggt gtctcatcat ccgagcgtac
     3961 aggattgtgg ccgatcgcgc gatgcacgcg tcaactgacc cggtgacctg gcaccacggc
     4021 acagaaccag gccatcaccc ggtgctcaca acccataagc caatgcacga ggctatggca
     4081 atgagcgaca ggacagtagc aatgacgctc aagcgtctga ggattcgcat gtcgcgttcg
     4141 attctggctg cgccagagtc gattccggtg atgacctgct gcatttgcgt ggccaggatt
     4201 ttcaccgact ctgcattgtg gaccaccgcg ctctgcagct cctgaatctg ctccgggata
     4261 acgtcttgtg cagttccggt cttgcgcgtg aacgcaggaa ttgccgcagt gacgatttgc
     4321 gtggcgtatg gaaggagtgc tttcaatgcg ggaagccatg cggccatacc gtctccaagg
     4381 cagtgcaggc aagatcactg atattaccaa atgccaatca cgcgcatctt tcgatggacg
     4441 tcgctccggc acccgccccg ccgccgcatt cgaccccgag caacaatcac tcttccaggc
     4501 taagattaca ccgggtaagg accgagtaat gcctcaggca gatcgatcaa gcgccgcggt
     4561 ttttgcatct gcaactgcca tggccatcac atagcagtct tcgagatcgg ttggtccttc
     4621 catcgacatt ttcagatccc gaataacgcc atcgttcagg tcatacaccc agccgtgcac
     4681 ggtcagttcc tgtccgcgcg cccatgcatc ctgcactacc gtggtctgtg cgacatttac
     4741 cgcctgctct atcacgttga gttcgcacaa gcggccgatg cgatgttccc ggctatcgat
     4801 cgccccgagt gcatcacgat gcttctgcgc gacatcctgc acgtgccgca gccaattgtc
     4861 gatcagccca agcttcaggt tgtcatgtgc tgcggtcacc ccgccacagc cataatgtcc
     4921 acagacgatg acgtgtttga ccttcagcac atcgactgca tactgcagca ccgaaaggca
     4981 gttcagatcg gcatgcacga cgatattcgc aacattgcga tgcacgaaga cctcacccgg
     5041 ggccaggccg gtgatctggt tagccggaac ccggctgtcc gagcagccga tccacaaata
     5101 ctccggcacc tgcagctgcg aaagtctggt aaaaaaatcc ggttcgcggc tgcgtatgct
     5161 cgccgcccag agccggttgt tagagagcag cttgtcgagt cttttcatgg caagtgtcta
     5221 ccgattttta cgtgacccag gctcgggccg gcaacgatac tgctgccact ttcggatttg
     5281 acggattcgg catcaggact ggcccgcttt gaggctgttg ctcagaaaac gatcgatatg
     5341 gttggcgaag gccatgcgat cggcattact gaagatggaa ggcccgccag tttcgatacc
//
LOCUS       DATMZQ010000245         5389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2951591, whole genome shotgun sequence.
ACCESSION   DATMZQ010000245 DATMZQ010000000
VERSION     DATMZQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951591"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1270)
                     /locus_tag="VN496_08025"
     CDS             complement(92..1270)
                     /locus_tag="VN496_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXF16937.1"
                     /translation="MDFGLPSARPGVKALRSSKIREVANAGMGKEDVIAFWFGEPDEV
                     TPEFIRRAGIEALERGETFYTQNLGIPSLREALASYVTRLHRAESMALSMDNIAVTSS
                     GMSALMLATQALVSPGDRVVIVTPVWPNLVEIPRIIGAEAVTFALTYSSAGWTLDLER
                     LLATLTPGTRMVYINSPNNPTGWTISNQEQRAILDHCRRHGIWILSDDAYERLYYGNG
                     IAPSFIGITDARDRIISANTFSKSWLMTGWRLGWLVVPSELTPDLGTLLEYNTSCAPS
                     FVQRAGVVAVEQGEAVIERTKARFHRARDFLIRELNTIPGVEATPPPGAMYAFFKVAG
                     AKDTLTFCKRLVAEVGLGLAPGIAFGPEGEGFVRWCFASDEDRLAQGIGRLKKFMAHR
                     "
     gene            1372..1974
                     /locus_tag="VN496_08030"
     CDS             1372..1974
                     /locus_tag="VN496_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXF16938.1"
                     /translation="MPADGSQDMRLRILDAALGLLAGQGFMEVTQPKIAKAAGVRQSH
                     LTYYFPTRSELLKALAVHSIESMLGSLAAGASTGKLTPALFAQLSGEALADKRRARVM
                     LGLIVSSEEDREIKQFMRDFVARVRVTLTGVARLLGREPDAKSIAALHMLFIGATVLN
                     VARDDANSRRECAEIARLAVETFLPGTPPATEPKARRRKS"
     gene            1971..2936
                     /locus_tag="VN496_08035"
     CDS             1971..2936
                     /locus_tag="VN496_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXF16939.1"
                     /translation="MNADQRSYRMICLVLLTSVLAACGQTKVPAYQGYVEGDYVLIAS
                     PYAGSLQSLNVARGQNVESGAALFTLEQDNEAAEKHEADEKLRAAQAKLENLRKGQRV
                     PELEALIAEERQAQAARKLSEVQYVRSQKLFEGGFISSSALDEAKANLARDRARAEQV
                     QAQIRVGKQSVGRSGELAAAQAEIDAAKAVVAQADWKLAQKAQRAPQAGLVQDTFFVQ
                     GEWVPAGKPVVSLLPPANIKLRFFVPETLVGQLKTSQRVTATCDGCSAPIAATISYIA
                     PNSEFTPPVIYSKESRAKLVFMIEAVPAAADAMKLKPGQPVDVTL"
     gene            2933..3859
                     /locus_tag="VN496_08040"
     CDS             2933..3859
                     /locus_tag="VN496_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF16940.1"
                     /translation="MNSELVIDVQNLNKRFGDKHVVRDFSLQVRRGEIFGFLGPNGSG
                     KTTSIRMLCGLLTPDSGSGTCLGYDVIRETAAIKREVGYMTQRFSLWEDLTIRENLDF
                     VARMYGMPRRGQIVDQAIAQLGLTARKDQLSGSLSGGWKQRLSLAACMLHHPKLLLLD
                     EPTAGVDPKARRDFWDEIHALAATGISVLVSTHYMDEAERCHRLAYIAYGAVLSRGTV
                     DEVVAAQHLSTWLVSGPDLPELEARLRGTPDISQVTPFGSTLHVVGVDAAALDAAIAP
                     FRADERYQWQRSHATLEDVFIHLMESAPDNFQ"
     gene            3856..4995
                     /locus_tag="VN496_08045"
     CDS             3856..4995
                     /locus_tag="VN496_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF16941.1"
                     /translation="MKISGFSLSRWLGIVVKEFIQLRRDRLTFGMIIGIPVLQLLLFG
                     FAINSDPKSLPTAVLSQDNSRYARTVTAALQTSGYFMVVTQIATEDEAERLLARGDVQ
                     FVVTIPVGFERALARGERPVLLVEADATDPAATGNALAALNQAIDTALAHDLTGPLAD
                     AVAKPAPVDVRIHRRYNAEGITSYNIVPGLLGVILTMTMVLMTGLAMTRERERGTFEN
                     LLATPALPIEVMTGKIVPYILIGLVQVTLVLLAARYIFTVPMQGNIAVLYAGVLIFIA
                     ANLTLGITFSSLARNQLQAMQMTFFFFLPSILLSGFMFPFRGMPHWAQLIGSALPLTH
                     FLTLVRGLMLKGNSFVDLWPSLWPILVFMAVAMAVGLKVYRRTLD"
     gene            complement(5105..5323)
                     /locus_tag="VN496_08050"
     CDS             complement(5105..5323)
                     /locus_tag="VN496_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16942.1"
                     /translation="MKTCTLFSALSDAQARLAATPVSPPKAVAAVALAAARDDTNLLA
                     ALRNSATDRDQIMQRRISPLTLEHDSIV"
BASE COUNT         1044 a         1631 c         1686 g         1028 t
ORIGIN      
        1 gcgcggtacg cggatgtgcc tttgccgaga ttgccggtcc agcggactgt gctttggtac
       61 tcgtgatgct ttgttgtgct gcccatgatc tttaacgatg tgccatgaat tttttcaatc
      121 gcccgatacc ttgcgccaac cggtcttcat cggacgcgaa acaccagcgc acgaaacctt
      181 cgccttcagg accgaatgca atcccgggag ccaggccgag gcccacttcc gcaaccagcc
      241 ttttgcagaa ggtcagcgtg tctttcgcgc ccgccacctt gaagaaagca tacatcgctc
      301 cgggcggtgg cgtggcttcc acgcccggaa tcgtgttgag ttcacgaatc aggaaatcac
      361 gcgcccgatg aaagcgggct ttcgtgcgtt cgattaccgc ttcaccttgc tccaccgcaa
      421 ccacgcctgc tctctgcacg aatgatggcg cgcaggacgt gttgtattcc agcaaggtgc
      481 ccaggtccgg tgtgagttcg ctgggcacga ccagccatcc taggcgccag ccggtcatga
      541 gccacgattt ggaaaaggtg ttcgcactga tgatgcgatc ccgcgcatcg gtgatgccga
      601 taaaagacgg ggcgatgccg ttgccatagt aaagccgctc ataagcatcg tcgctgagga
      661 tccagatgcc gtgccggcgg cagtgatcca gaatcgcgcg ctgctcctga ttcgaaatgg
      721 tccagccggt cggattgttc ggcgagttga tgtagaccat ccgtgtgccc ggggttagcg
      781 tagcgagcag gcgttcaaga tcgagcgtcc aacctgctga ggaataggtc aaggcaaaag
      841 tgaccgcctc cgcaccgata atccgaggaa tctccaccag gttcggccac accggcgtga
      901 cgatgacaac gcgatctccc ggactgacca gcgcctgcgt cgcgagcatc agcgccgaca
      961 tgccggaact ggtcacggca atgttgtcca tggacagcgc catgctttct gcacgatgca
     1021 ggcgagtgac atagctggcg agcgcctcgc gcagggatgg gatgccgaga ttctgggtat
     1081 agaacgtctc gccgcgttcc agcgcctcga tgcccgcccg gcggatgaat tccggcgtga
     1141 cttcgtcggg ctcgccgaac cagaacgcga tcacgtcttc cttgcccatg cccgcattgg
     1201 ccacctcacg gattttcgag gagcgcaggg ccttgacgcc gggacgagca gagggaagac
     1261 cgaaatccat atgggttcca ggcaaaggac ggcattttgg cataccgcca accttgacat
     1321 cccttggtag ttataccaaa ctgtttggta aatataccaa ggagcgggac gatgccggcg
     1381 gacggctcac aggatatgag gctcaggatt ctggatgccg cgctcggcct gctggccggg
     1441 caggggttca tggaggtcac gcaacccaag attgccaagg ccgccggcgt gcgccagagc
     1501 cacctgacct actacttccc gacccgcagc gaattgctca aggcgctcgc cgtgcattcc
     1561 atcgaatcca tgctcggttc gcttgcggcc ggcgcatcca cgggaaagct cacgcccgcc
     1621 ctattcgcac agctttccgg cgaagctctg gcagacaagc ggcgcgcccg ggtcatgttg
     1681 ggcctcattg tgagctcgga ggaggaccgg gagatcaagc aattcatgcg cgatttcgtc
     1741 gcacgcgtgc gggtgaccct gaccggggtc gccaggctgc tcggccggga gcccgatgcc
     1801 aaaagcatag ccgccttgca tatgttattt atcggcgcca ccgtgctgaa cgtcgcacgg
     1861 gacgatgcca attccaggcg cgaatgcgcc gagatcgcgc gccttgcggt ggaaacgttc
     1921 ctgcccggaa ccccgccggc aaccgagccg aaagccagac ggagaaaatc atgaacgccg
     1981 accagcgcag ttaccggatg atttgcctcg tgctgctcac gtccgtgctg gcggcctgcg
     2041 ggcagaccaa ggtgccggct tatcagggct atgtcgaagg cgactacgtg ctgattgcat
     2101 cgccctacgc tggttcattg cagtcgttga acgtagcccg cgggcagaac gtggaaagcg
     2161 gtgcggcact gtttacgctg gaacaggata acgaggctgc ggagaaacat gaggccgatg
     2221 aaaagctgcg cgccgcgcag gcaaaactgg agaatctgcg caaaggccag cgcgttccgg
     2281 aactggaggc gttgatcgcg gaagagcggc aggcgcaggc cgcccgcaag ctctcggaag
     2341 tgcaatacgt gcgcagccag aaattgtttg aaggtggctt catctcttca tcggcactcg
     2401 acgaggccaa ggccaacctc gcacgcgacc gcgcgcgcgc cgagcaggtg caagcccaga
     2461 tccgcgtggg aaagcagagc gtgggccgat cgggcgagct cgcggcggcg caggcggaaa
     2521 tcgatgccgc caaagcggtg gtcgcgcaag ccgactggaa actcgcgcag aaggcgcagc
     2581 gcgcgccgca ggccggcctg gtgcaggata cgtttttcgt gcagggcgaa tgggtgcccg
     2641 cgggtaaacc ggtcgttagc ctgctgccgc ccgctaacat caagctgcgc ttttttgtgc
     2701 cggaaacgct ggtcggccaa cttaaaacca gccagcgcgt gaccgcgact tgcgacggct
     2761 gcagcgcgcc gatcgccgcg accatcagct acatcgcgcc gaattcggaa tttacgccgc
     2821 cggtcattta cagcaaggaa tctcgtgcca agctggtatt catgatcgag gcggttccgg
     2881 cggctgccga tgccatgaag ctcaagcccg gccagccggt ggacgtgaca ctgtgaattc
     2941 cgaattggtc atcgacgtgc agaacctgaa caagcgcttc ggcgacaagc atgtcgtgcg
     3001 cgatttttcc ctgcaggtca ggcgcggcga aatcttcggc ttcctcgggc ccaacggcag
     3061 cggcaagacc acctcgattc gcatgctgtg cggattgctg acgccggaca gcggcagcgg
     3121 tacctgcctt ggctacgacg tgattcgcga gaccgccgcc atcaagcgcg aggtgggcta
     3181 catgacccag cgtttctcgc tgtgggaaga cctgaccatc cgcgagaacc tggatttcgt
     3241 tgcacgcatg tacggcatgc ccaggcgcgg gcagatcgtg gatcaggcga ttgcacaact
     3301 cggtttgact gcacgcaaag accagttgtc cggttcgcta tccggtggat ggaagcagcg
     3361 cctgtcgctg gcggcatgca tgctgcacca cccgaagctg ctgctgctcg acgaacctac
     3421 ggccggcgtc gatccaaaag cacggcgtga tttctgggat gaaatccatg cgctggcggc
     3481 gaccggaatc tcggtactgg tctccacgca ctacatggat gaagcggagc gttgccaccg
     3541 gctggcatac atcgcctatg gggcggtgct gtctcgaggc accgtggacg aagtagttgc
     3601 cgcccagcat ttgtcgacgt ggctggtgtc gggacccgat ctgccggaac tcgaagcgcg
     3661 cttgcgcggc acgcccgata tcagccaggt cacgcctttc ggttcgactt tgcacgtggt
     3721 cggcgttgat gccgcggcgc tggacgcggc tatcgcgccg ttccgggcag acgaacgcta
     3781 tcaatggcag cgaagccatg ccacgctcga agacgtattc atccacttga tggaatcggc
     3841 gcccgacaat ttccaatgaa gatcagcggt ttttccctgt cgcgctggct gggcatcgtg
     3901 gtgaaggaat tcatccagct gcggcgcgac cgcctgacct tcggcatgat catcggcatc
     3961 ccggtgctgc agctgctgct gtttggtttt gccatcaact cggacccgaa gtcgctgcct
     4021 accgcagtgc tgtcacagga caacagccgt tacgcgcgca ccgtaacggc cgcgctgcag
     4081 accagcggct acttcatggt cgtcacgcaa atcgccaccg aagacgaagc cgaacgtctg
     4141 ctggcgcgcg gcgatgtgca attcgtggtc accatcccgg tgggattcga gcgtgcgctg
     4201 gcgcgcggcg agcggccggt actactggtc gaagcagatg cgaccgatcc cgcggcgacc
     4261 ggaaacgcgc tcgctgcatt gaaccaggca atcgataccg cgctggcgca tgacctgacc
     4321 ggcccgctcg ccgatgcggt agcgaagccc gcgccagtgg atgtgcggat ccatcgtcgc
     4381 tacaacgcgg aaggcataac ctcgtacaac atcgtgcccg gattgctggg cgtgattttg
     4441 accatgacca tggtgctcat gaccggactg gccatgacac gcgagcgcga acgcggcacc
     4501 ttcgaaaacc tgctcgccac gccggcactg ccgatcgagg tcatgaccgg gaagattgtg
     4561 ccgtatatcc tgatcggact ggtccaggtc accctggtgc tgctggcggc gcgctacatt
     4621 ttcactgtgc ccatgcaggg caacatcgcg gtgctctacg cgggggtgct aatcttcatt
     4681 gcggccaatc ttacgctcgg tattacgttc agttcgttgg ctcgcaatca gttgcaggcg
     4741 atgcagatga catttttctt ctttctgccg tcgatcctgc tttccggatt catgtttccg
     4801 tttcgcggca tgccgcactg ggcgcagttg atcggcagcg ccttgccgct aacgcatttc
     4861 ctgaccctgg ttcgggggtt gatgctgaag ggcaattcct tcgtggatct ctggccgagc
     4921 ctgtggccca tcctggtatt catggccgtc gcaatggccg tcggcctgaa ggtatacaga
     4981 aggacactgg attgagccgc ggcttagtac gtgacggata aggccccagg ataaaaaaag
     5041 gccggctcct ggactaagag ccggccgaag cggcgggacc aggcggcccc gcccgcacag
     5101 acgttcaaac aatgctgtcg tgctcgaggg tcagcgggga gatgcgccgc tgcatgatct
     5161 gatcgcgatc ggtagcgctg ttacgcaatg cagccagcag gttggtatcg tccctggccg
     5221 cggcaagggc aaccgcggct acggctttcg gtgggctgac gggagtggca gccaaacggg
     5281 cttgtgcatc ggacaacgcg ctgaacaagg tgcaggtctt catcttcggg cctttccgtc
     5341 gcggtcatcg ttgatcggtg agcggcgcca tgttgcgcac ccctggtcc
//
LOCUS       DATMZQ010000246         3426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1917004, whole genome shotgun sequence.
ACCESSION   DATMZQ010000246 DATMZQ010000000
VERSION     DATMZQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917004"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VN496_08055"
     CDS             complement(<1..1259)
                     /locus_tag="VN496_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HXF16943.1"
                     /translation="MSTRRQFLRSSGAALLGAGLISRLQAASLPEAPIQMSPLTQPPL
                     APPDGRPYHPVVTLNGWTLPWRMNNGVKEFHLIAEPVVRELAPGMKANLWGYNGQSPG
                     PTIEVVEGDRVRLFVTNKLPEHTTIHWHGQRLPNGMDGVGGLTQPHIKPGQTYVYEFE
                     ARRAGTFMYHPHADEMTQMAMGLMGSWVTHPKNPDLHRVDRDFIFLLNAYDIDPGSFT
                     PRTSTMLNFNLWNFNSRVFPGIDAMVARKGDRVRVRVGNLTMTNHPIHVHGHEIEVAG
                     TDGGWLPTSARWPEVSIDIGVGQMRAIEFDATEEGDWAFHCHKAHHTMNAMGHNVPTM
                     IGVDQRNVAEKITRLVPDYMVMGDTGNAMGEMRMPLPDNTLPMMTGTGPFGPMEMGGM
                     FTTVKIRAGLEAGDYKDPGWYKHPDGTV"
     gene            complement(1262..2830)
                     /locus_tag="VN496_08060"
     CDS             complement(1262..2830)
                     /locus_tag="VN496_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXF16944.1"
                     /translation="MRFFLLCSALSAIKGYSATSALPGFSAGLATSPTKGIRPRITVR
                     FITTITALISLLLGGCASFSRDGGFDTVAATSHERINKDVTWVRGDAEAASVRKSVEA
                     LLASPLTADQAVQVALLSNPGLQATYAELGIAEANLVQAGRLRNPGFTFLRAHSDEGT
                     RIERTYTFDFASLIMAPLATRMEGRRFEQTKLLVTEEVLRVAADTRKAYFAALAAQEA
                     LRYFEQVKIAAEAGSELAQRMANAGNWSKLDQAREQVFYAEAIAQLARAQQTGIAERE
                     RLTRLMGLSGADVAFKLPERLPALPKDKPDLKDLEPYAIRERLDIRSAQRNAEALAAS
                     LGLTKTTRFINVLDVSYLRNSEVGGVRETGYEISLEIPLFDWGGARVAKAQAIYMQAV
                     DRVAETAVNARSEVRESYASYLNAYELARHYRDEIVPLRRTISEENQLRYNGMLISVF
                     ELLADAREQVTSVNAYIEALRSFWLAETELQRILGGRLPVSLADAGPQANPQAPVAPD
                     AAEQSNHQQHQKGK"
     gene            complement(2929..>3426)
                     /locus_tag="VN496_08065"
     CDS             complement(2929..>3426)
                     /locus_tag="VN496_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16945.1"
                     /translation="IDSALRRGFNDLPHVLALKLILRELLLLVKSLRLLMIGWMVLWL
                     AGNGVLAVAMPYCKHAAGEHGDAAARVQDRVLAAQQAVHAAHQHDHHQHSANTQGHNG
                     DHKALSVFCDSCDLCHLAASAVPVAASADGLAPAEQIHPIATDVTIPSRFIELPQPVP
                     LATRA"
BASE COUNT          664 a         1034 c         1000 g          728 t
ORIGIN      
        1 accgttccgt ccggatgttt gtaccatccc ggatccttgt agtcacccgc ctcaagcccg
       61 gcgcgaatct tcacggtagt gaacatgccc cccatttcca tcggtccgaa cggtcccgtt
      121 ccggtcatca tgggcaaggt attgtcgggc agcggcattc gcatttcgcc cattgcgttt
      181 ccggtgtcgc ccatcaccat gtagtccggc acgagtctgg tgattttttc cgctacgttg
      241 cgttgatcga caccgatcat ggtcggaacg ttgtgcccca tggcattcat cgtgtgatgc
      301 gccttgtggc agtggaacgc ccagtcgccc tcttccgtgg catcgaattc gatggcccgc
      361 atttgtccga cgccgatatc gatgctgact tccggccagc gcgccgacgt gggcagccaa
      421 ccgccatcag tccccgccac ttcgatttcg tggccatgta catgaatcgg atggttcgtc
      481 atggtcagat tgccaaccct gacgcgcacc cggtcgcctt tgcgcgcaac catggcgtca
      541 atgccgggaa agacgcggct gttgaaattc cacaaattga aattgagcat ggtgctcgtg
      601 cgcggtgtga aacttccggg atcgatgtcg taggcattca gcaaaaaaat aaaatcgcga
      661 tccacacgat gcaagtcagg atttttcggg tgtgtgaccc aagagcccat cagacccatg
      721 gccatctggg tcatctcgtc ggcgtgcggg tggtacatga acgtacccgc gcgccttgcc
      781 tcgaactcgt acacgtaggt ctggccaggc ttgatgtgag gctgcgtcaa cccgcccacc
      841 ccgtccatgc cattcggcag ccgctgacca tgccaatgga tcgtcgtatg ctcgggcaac
      901 ttgttggtta cgaacaaccg caccctgtcg ccttcgacca cctcaatggt cgggcccggg
      961 ctttggccgt tgtagcccca caggttcgct ttcattcccg gcgcaagctc gcgcaccacc
     1021 ggctcggcaa tcagatgaaa ttctttgacg ccgttgttca tgcgccacgg cagcgtccag
     1081 ccattcaggg tgaccaccgg atggtaaggt cgtccgtccg gcggagccaa cggcggctgc
     1141 gtcaacgggg acatctggat tggggcttcg ggcagcgaag cggcctgcag cctgctgatc
     1201 aggccagctc ccagcagcgc ggctccagat gagcgcaaaa attgtcgtcg tgtggacatg
     1261 gtcactttcc tttttggtgc tgctgatgat tcgactgttc cgctgcatcc ggtgcgactg
     1321 gcgcttgggg attggcctgc gggcctgcat cggcaagcga tacgggaagc cggccgccaa
     1381 gaatgcgttg aagctcagtt tccgcaagcc agaagcttct gagtgcttcg atataagcgt
     1441 tgacgctggt cacctgctct cgcgcatcgg cgagcagttc aaacacgctg atcagcatgc
     1501 cgttgtagcg caattgattc tcttcggaga tggtcctgcg caacggcacg atttcgtcgc
     1561 ggtagtgcct ggccagttca taagcattca aataactcgc gtaagactcg cgcacttcgg
     1621 agcgcgcgtt gactgcggtt tcagccaccc ggtcgaccgc ctgcatgtag attgcctgcg
     1681 cctttgcgac tctcgcgccg ccccaatcaa aaagtggaat ttccagactg atctcgtagc
     1741 cggtttcgcg cacgccaccg acttcgctgt tcctcaagta actcacatcc agcacgttga
     1801 tgaagcgagt cgtcttggta agaccaagag atgccgccaa cgcctcggcg ttgcgctgcg
     1861 ccgagcggat gtcgagcctc tcccgaatgg cgtagggctc cagatccttg aggtcgggct
     1921 tgtccttggg cagcgcggga agccgttcgg ggagcttgaa tgccacatcg gcgcccgaca
     1981 accccatgag gcgggtcagt cgttcccgct cggcaattcc agtttgttgt gcgcgcgcca
     2041 attgcgcgat ggcttcagcg tagaacactt gctcacgcgc ctggtcaagc ttgctccagt
     2101 tgcctgcgtt ggccatgcgc tgcgccagtt ccgacccggc ctcggccgca atcttgacct
     2161 gctcgaaata gcgcaaggcc tcctgcgcag ccaacgcggc gaagtaagct tttcgtgtgt
     2221 cggcagcaac ccgcagcact tcttcagtga ccagcagttt ggtctgctcg aagcgacggc
     2281 cttccatgcg cgttgccagc ggcgccatga tgaggctggc gaaatcgaac gtgtaagtcc
     2341 gttcaatgcg cgttccttca tccgaatgtg cacgcaagaa ggtgaacccc ggattgcgca
     2401 ggcggccagc ctggacgaga ttagcttccg cgataccgag ctcggcgtaa gtcgcctgca
     2461 gcccagggtt gctcagcaat gcaacctgta ctgcctggtc cgccgtcagt ggcgacgcga
     2521 gcaatgcttc aacacttttt ctaacggagg ccgcttcggc gtcaccgcgc acccaggtta
     2581 cgtccttgtt gattctttca tgagacgtcg cggcgacagt atcaaatcct ccatcgcgcg
     2641 aaaaactggc gcacccgccc agcagcaggg atatcagcgc cgtaatcgta gtgatgaacc
     2701 gaaccgtgat gcggggtcgt atgcctttcg tggggctggt tgccagcccg gcactgaaac
     2761 cgggcaaggc cgatgttgcg ctgtagccct tgatcgcgga cagcgccgaa cacaacagaa
     2821 aaaatcgcat ggtttccctc gtgtgacatg agcgagatgt gccgaacgca ggctatggcg
     2881 ccaagcaaaa cgctcggccc tcatgcggac ggctttccaa cgggcgcttt aggcgcgcgt
     2941 ggctagaggg acaggctggg gcagttcgat gaatcgcgac gggatggtga catcagtcgc
     3001 gatcggatga atttgttcag cgggcgcgag tccgtcggct gaagcggcaa cgggaacagc
     3061 tgaggctgcg aggtggcata aatcacagct atcgcaaaaa acgcttagcg ctttatggtc
     3121 accattgtgg ccctgtgtgt tcgccgaatg ctgatgatga tcatgttgat gagcggcatg
     3181 cactgcttgc tgtgccgcca gcacccggtc ctgtacacgc gcggccgcat cgccgtgctc
     3241 accggctgcg tgtttgcaat acggcatggc aaccgccagc acgccgttac cagcgagcca
     3301 gagcaccatc catccgatca tgagcaggcg aagcgatttc acgagcagaa gcaattcccg
     3361 gagaataagt ttgagggcga gtacatgcgg caagtcatta aaaccgcgcc gcagcgcact
     3421 gtcaat
//
LOCUS       DATMZQ010000247         4774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3296248, whole genome shotgun sequence.
ACCESSION   DATMZQ010000247 DATMZQ010000000
VERSION     DATMZQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3296248"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VN496_08070"
     CDS             complement(<1..371)
                     /locus_tag="VN496_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXF16946.1"
                     /translation="MLLFCWISAAAAQPGGPVVILTQDGAIGPATADYLHRGMEHAAK
                     LHAQLVVLRMDTPGGLDLSMRSIIRDILASPVPVASYVAPDGARAASAGTYILYASHI
                     AAMAPSTNLGAATPVELGGAD"
     gene            688..1395
                     /locus_tag="VN496_08075"
     CDS             688..1395
                     /locus_tag="VN496_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16947.1"
                     /translation="MFGSDDLWLRVKRWGPGRAARDSMRRMRKRLWFFDEIRILKKNV
                     VRLPVHRSLDVTAHDDLQHIMELNQATDHATTEHRIRQRLRHGLRFYAATVRGKPVAT
                     TWILLPGQRFFDECGYAIDVPADDLWLRDIHVAPWCKGRRVFSAFLDHIVKTFHPTVR
                     HLWSDAELNTPASLNAHRNYGFQTVCTLKVLHLAECMMVRTLPSSPARCAYGFKLPAR
                     VLATGPRYQTYKAIHLM"
     gene            1464..2627
                     /locus_tag="VN496_08080"
     CDS             1464..2627
                     /locus_tag="VN496_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF16948.1"
                     /translation="MDFSLTDEQEQLRDSARKFAQSELPALARTLEEKNEPVPDSMME
                     RYAALGYLGINLPEAYGGHGLSHLDAVIVLEECAKISPAVAFPVFESCFGPILAIAHF
                     AADTLRKRLIPQVVAGKQIVAVAMSEPDAGSALTDLRTHARLDGDSVRLSGQKRWCSG
                     AGHAGGYLVYCKMSDEPGARGIGAVYVEKGSKGFSFGKREQLMGFRGIASADMFFDDV
                     RVPVENVVVAAGDGFRKLMEAFDLERCGNATMSLAIAQGALDYVLDYVQERNQFGKPI
                     VDFQAVQLRLADMAMKLEASRLLIYQAVWGASAGLPSIRQSSIAKCFANEMVREVTGH
                     ALQMMGAYGYSKDYPLEQRMRDAWGWGIAGGAIDIQKTNIASALIGRRFDQRR"
     gene            2752..3960
                     /locus_tag="VN496_08085"
     CDS             2752..3960
                     /locus_tag="VN496_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXF16949.1"
                     /translation="MKIKSIEALACDAGWRNYHFVKITTDTGIVGWSEFDEGFGAPGV
                     GAVIDRLAARALGQDVNAHERIYAELYCATRPGAGGVVGIAMGAIENAMLDAKAKALG
                     VPAYQLLGGKIRDRIPVYWSHCATWRIFQPDYYRPPITSIEGVRSIGREVREKGFRAL
                     KTNIFLYDADGGNARGWGPGFALPFSPELNVDRTILRNLRRHLEALREGAGPDIDILL
                     DLNFNAKTEGFLSILRALADFELFWVEIDSYDPAALGYIRRQSPHPISSCETLMGLRE
                     FLPYFREQAMDVAIIDVPWNGAWQSMKIAAAAEAHEVNVAPHNFYGHLCTMMNAHFAA
                     AVPNLRIMEVDIDRIAWDHELFSHSPQFVDGHLVLPDRPGWGTEPIEAALRAHPPTAR
                     RGIVSRTAND"
     gene            complement(4012..4620)
                     /locus_tag="VN496_08090"
     CDS             complement(4012..4620)
                     /locus_tag="VN496_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16950.1"
                     /translation="MLPKVIIAIALLTALPAIAGDKDYALTVYGGYRGGGHFTNTNTG
                     ESLSLQSNGSASLALDLPLDEARQYQVFASYQRTNLTLDSTLLTGSTKLAMDIGYLHF
                     GGSYFWEGQVSKGGYLVGGIGATMFNPAQGLKTELYPSINVGVGYQLPLGKTFAMRFE
                     ARGYATLVNSQSGVFCSGGCVVSIKGDTITQGEALLGLTARF"
BASE COUNT          965 a         1414 c         1449 g          946 t
ORIGIN      
        1 ttatctgcgc cgcccaactc caccggcgtg gcggcgccca ggttggttga gggcgccatc
       61 gccgcgatgt gactggcgta caaaatgtac gtgccggcac tggcagcgcg cgcgccgtcc
      121 ggcgcaacat agcttgccac aggcaccggg gaggcgagga tgtcgcgaat gatggagcgc
      181 atggacaggt ccagtccgcc gggtgtgtcc atgcgcaata cgacaagttg ggcatgcagc
      241 ttggcggcgt gctccatgcc gcgatgcagg taatcggctg tggcggggcc gatggcgccg
      301 tcctgcgtaa ggatcacaac cggcccaccc ggctgcgctg ccgcagcaga gatccaacaa
      361 aacaacagca ttacgatcac acgcaccggc ccacccgtat ggatgtctgg cggcgccgct
      421 ccatccggga aaattacaac ccggaaccgg cggccgctca gcgccagagt aacggaattg
      481 gcgttgcctg gcacgagtaa ctatgggatg aacgatgcat tgccattgcg agattccgtt
      541 ggctgacgcc catcggctca tgacgctcgc gaaatccatt ggaacaacat cccggctctg
      601 tactccaatt tcacgctggg cattcaggct tcccggccct ccgaagcatt gaataacgaa
      661 ttggcaatct atcgcccgag gatcaaattg tttggatccg acgatttgtg gttgcgggtg
      721 aagcgttggg gtccgggccg cgccgcccgg gatagcatgc gccggatgcg caaacggctg
      781 tggttcttcg atgaaatccg catcctgaaa aaaaatgtcg tccgcctgcc ggtccaccgg
      841 tcgctcgacg taaccgcgca cgatgatcta cagcacatca tggaactcaa ccaggccacc
      901 gaccatgcga caactgaaca tcgcatccgc cagcgccttc ggcacgggct acgcttctat
      961 gccgcaacag tgcgcggcaa gccggtcgcc acaacctgga ttctgttgcc gggacaacgg
     1021 tttttcgacg aatgtggtta cgctattgac gttccagccg acgacctgtg gttacgggac
     1081 attcatgtcg cgccctggtg caaagggcgg cgcgtgtttt ccgcattcct ggatcacatc
     1141 gtgaaaacat tccacccgac ggtgcgccac ctgtggtcgg acgccgaact gaacacgcca
     1201 gcatcattga atgcgcaccg taactacggc ttccagaccg tctgtacact gaaagtgctg
     1261 catttggccg agtgcatgat ggtgcgaaca ttgccgagca gtccggcacg ttgcgcatac
     1321 gggttcaaac ttcccgctcg cgtacttgca accggaccgc gctaccagac ttacaaggcc
     1381 atccacttga tgtaagggag cggaatccga ccttgactgt cgggacatgt ggccggctta
     1441 taatccccga aaagggagcg gccatggatt tcagtctcac cgatgagcag gaacagctgc
     1501 gcgactcggc gcgcaaattc gcgcaatcgg aattgccggc gctggcacgc acgctcgaag
     1561 aaaaaaacga gccggttccg gacagcatga tggagcgcta tgccgcgctc ggttaccttg
     1621 gcatcaatct tccggaagca tatggcgggc atggactttc ccatctcgat gccgtgatcg
     1681 tgctggaaga gtgtgcaaaa atttcaccgg cggttgcttt tcccgtgttc gaatcgtgct
     1741 ttggccccat cctcgccatt gcgcatttcg cggccgacac gctgcgcaag cggctcatcc
     1801 cgcaagttgt cgccggcaag caaatcgtgg cggtcgccat gtcagagccg gatgccggct
     1861 cggcgctgac cgacctgcgc acgcacgcgc gcctggatgg cgacagcgtt cgcctcagcg
     1921 ggcagaaacg ctggtgctcg ggtgctggcc acgccggggg ttatctggtc tattgcaaaa
     1981 tgtccgatga gcccggcgcg cgcggcatcg gtgccgtcta tgtcgaaaaa ggcagcaagg
     2041 gattcagctt cggcaagcgc gagcagctga tgggcttccg gggcattgcg tccgcagaca
     2101 tgtttttcga tgatgttcgc gtgcctgtgg agaacgtggt ggtcgcggct ggcgatggct
     2161 ttcgcaagct catggaagcc ttcgacctgg aacgctgcgg caacgccacc atgagcctcg
     2221 ccattgcgca aggtgcactc gattacgtgc tcgactacgt gcaggagcgc aatcagttcg
     2281 gcaaaccaat cgtagatttc caggcagtgc agctgcggct tgcagacatg gccatgaaac
     2341 tggaggcgtc acgcctgttg atctaccagg cggtatgggg tgcatccgca gggctgcctt
     2401 ccatccggca aagctcgatc gccaaatgct tcgccaacga gatggtgcgc gaggtcaccg
     2461 gccacgcgtt gcagatgatg ggtgcatacg ggtacagcaa ggactaccct ctcgaacaac
     2521 gcatgcgtga cgcctggggc tggggcattg ccggcggcgc catcgatatc cagaagacca
     2581 acatcgcctc ggcactgatc ggccggcgtt tcgatcagcg tcgttgaacg acagcgatgt
     2641 cgcgcataga gtgagtcgtt cagtgcaatc gtattcgttt ttcgccgcgg acttcgcgtg
     2701 caatttctgt tagttcgcgc caaacatgtt ttcacgtacc gccggacaac catgaagata
     2761 aaaagcattg aggccttggc atgcgatgcg ggctggcgca actaccattt tgtcaaaatc
     2821 accacggaca ctggcatcgt tgggtggagt gagttcgacg aaggtttcgg tgcacctggc
     2881 gtgggcgccg tcatcgaccg gctcgccgcg cgggccctcg gccaggatgt caatgcccat
     2941 gagcgcatct acgccgagtt gtattgcgcc acgcggccag gcgccggagg cgtcgtgggg
     3001 attgccatgg gcgcaatcga gaacgccatg ctggatgcga aggcaaaggc actgggcgtg
     3061 cccgcatacc aactgctcgg cggcaaaatc cgtgatcgaa ttcccgtgta ctggtcgcac
     3121 tgcgccacct ggcgcatctt tcagcccgat tattaccgcc cgccgatcac cagcatcgag
     3181 ggcgtgcgtt cgatcggccg cgaagtacgt gaaaagggat tccgcgcgct gaagaccaac
     3241 atttttctgt atgacgccga cggcggcaac gcgcgtggct gggggccggg attcgcgctg
     3301 ccgttttcac cggagctcaa cgtcgaccgc acgatccttc gcaatctgcg ccggcatctg
     3361 gaagcgctgc gcgagggcgc cgggccggac atcgacatcc tgctcgatct gaacttcaac
     3421 gcgaagaccg agggctttct gtcgatcctg cgcgcgctgg ccgacttcga attgttctgg
     3481 gtggaaatcg acagctacga ccccgctgcc ttgggctata tccggcggca aagtccgcac
     3541 ccgatcagct cctgcgaaac gctgatgggg ctgcgagagt tccttcccta tttccgtgaa
     3601 caggcgatgg acgtggcgat tatcgatgtg ccgtggaacg gagcatggca atcgatgaaa
     3661 atcgctgccg ctgcagaagc gcatgaagtc aatgtggcgc cgcataattt ttacggacac
     3721 ctgtgcacaa tgatgaacgc ccatttcgct gccgccgtgc cgaacttgcg catcatggaa
     3781 gttgacatcg accgcattgc atgggaccac gaattgttca gccactcgcc acagttcgtg
     3841 gatgggcatc tggtattgcc cgatcggccc ggctggggaa ctgaacccat cgaggctgcg
     3901 cttcgggcgc atccgccgac ggcccgtcgc ggcatcgtga gccggacagc taacgactag
     3961 ctagggtgtt cgtaaaagcg gccttttgtc gcggacgaat tcccgacgcg ctcaaaatcg
     4021 cgccgtcaag cccagcagcg cttcgccctg cgtgatggtg tcgcccttga tggagaccac
     4081 gcaaccgccg gaacaaaaca caccgctctg gctgttgacg agtgtggcgt aaccgcgtgc
     4141 ctcgaaacgc atcgcaaaag ttttgcccag cggcaactga tagccgaccc cgacgttgat
     4201 cgacggatac agctcggtct tgaggccttg tgccgggttg aacatcgtcg cgccgatgcc
     4261 tccgacgagg tatccaccct tgctgacctg gccctcccaa aaataactgc cgccgaaatg
     4321 caggtagcca atgtccatcg ctaattttgt gctgccggtc agcagcgtcg agtcgagcgt
     4381 caggttggtg cgctggtagc tcgcgaacac ttggtactgc cgcgcttcgt cgagcgggag
     4441 gtccaacgcc agcgaagccg aaccgttgga ttgaagcgag agcgattccc ccgtgttggt
     4501 attggtgaaa tggccaccgc cgcgatagcc accgtacacc gtgagggcat agtctttgtc
     4561 acccgcgatg gcgggcagcg cggtcagcag agcgattgcg attatgactt ttggcagcat
     4621 cggcgacaga cggaattatc agtggaaacc catattgcct tgatccgcgt ttgctggcga
     4681 gcacggaata agatgccatt acatttatgg gcaccggccc ggttatgatc gaatgatcgg
     4741 ttgtggcgaa gccggttatg gcgtttggtc aatg
//
LOCUS       DATMZQ010000248         3259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2168925, whole genome shotgun sequence.
ACCESSION   DATMZQ010000248 DATMZQ010000000
VERSION     DATMZQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168925"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1625)
                     /locus_tag="VN496_08095"
     CDS             complement(276..1625)
                     /locus_tag="VN496_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXF16951.1"
                     /translation="MTPANRFLLMFLLVCMCAASPGTLAQQPAPLNLAEALRLALVNR
                     PLVKAGNLNVAAAKQNTIQVRSARYPLLIGSITAADAYQEHTTQGGKDITLDTRIAAG
                     GLNNPIILRRESNGIFLSQLVTDFGRTSSLVESAQLSEVSQQHQADAIRTEVVLEVSD
                     AYFSALEAQAVLRVAIKTVDARTAIFDRIASLAKNKIKSELDVRFAQVNLDEARLLLL
                     RAENALDAAFARLSTSMGDRDTRRFQLEEVPSVAPVGPLETLIEQAIANRPDLASLRA
                     DREAARKTVDAAKALAYPTISLYAAAGVTPYGDDRLSRNYGAVGLNLSVPFLDGGKIS
                     ALQQQAELRALAIGENIAEAENNVLRAVRVAWLNARSGYENIAITEHLRDAASEALAL
                     ADSRYKLGITSIVELNQAQLSAIDAEIGYARARYQYFSSRAILDFQAGSFEQAGTLR"
     gene            complement(1649..>3259)
                     /locus_tag="VN496_08100"
     CDS             complement(1649..>3259)
                     /locus_tag="VN496_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF16952.1"
                     /translation="NQKFQAMLKLYDVAVGHVLRHPTVTLVGFLGLFVGSLALLPFLG
                     MAYFPRTDPGQFVINLKTPTGTNVDVTEQAVAKIESIVKQEVGAKDLRLIVSNIGSVP
                     GFSSMYTPNSASHTAFVQVSLNEEHDVGSYEYMDKVRARITRELPQITVYFQSGGLVD
                     AVLNLGLPTPIDVQVSGSNLEAAHDTAVKLADSIRRLPGVNDVLVAQDIDAPSLRMDI
                     DRVQAAKLGLTQKEVVSNIITALTSNAMIAPSYWVDQRSGNDYLLTVQYPEQSVENIG
                     DLKAIPIRASNRTDSVRLDAMAKLNVIRAPTEVGHYQLRRVVDVYIAPAKENIRELVS
                     DVEHIVSQSVLPEGVRVALRGTVQSMRASFSSFGLGLLLSVVLVYLVLVAQFASFLDP
                     LLILLAIPTGLTGVLVTLLLTGTTLNVMSLMGVVMMVGIVVSNSILIVEFTRHLRSDG
                     VPLREAVALACRVRLRPVLMTSLATIIGLIPMAAKLGTGSEAYAPLARAIIGGLSISV
                     ITTVFIIPAAYLLAHRRREVPVGSPESA"
BASE COUNT          725 a          952 c          890 g          692 t
ORIGIN      
        1 cgggatttgc gcgagttcgc tgccatacgc ctggccgact tcaaagtacc ccgcaagatc
       61 gtcattctcg ccgagatccc gaaaggtgcc accggcaagc tgcagcgcat cggccttgcc
      121 cagaagctgg ggcttgcgta atacgcgaat acgccgtcgc cttcaacacc gtaatggaat
      181 ccgccgccgc gtaccccaat agtccgggtg cgatggcccg catgttcgcg cactagcggg
      241 cgcgcgcccc gcaacgccgt gcgaggattt ttcgactagc gcagcgtgcc ggcttgttcg
      301 aaggaacctg cctggaaatc tagaatcgca cgcgaggaga agtactggta acgggcccgc
      361 gcataaccta tttccgcatc tatcgcgcta agctgcgcct gattcagttc gacgatggag
      421 gtgatgccaa gcttgtatcg agaatcggcc aatgccaatg cctcggaggc cgcatccctc
      481 aagtgttcgg ttatggcgat gttttcgtat cccgatcgtg cgtttagcca ggccacacga
      541 accgcgcgta aaacattgtt ctcggcttca gcaatgtttt ctccaatcgc cagcgcccgc
      601 aactcggctt gttgctgcaa tgctgatatc tttcctccat cgaggaaagg tacactcaaa
      661 ttcaagccaa ccgccccata gtttctcgac aagcgatcat ccccgtaagg cgtaacaccg
      721 gctgcggcgt agaggctgat ggtgggataa gcaagcgctt ttgccgcatc aactgttttt
      781 cgtgcagctt cgcgatcggc gcgcagactg gcgagatccg gtcgattggc gatggcctgt
      841 tctatcagcg tttccaacgg gccgacgggc gctaccgagg gtacttcctc caactggaaa
      901 cggcgcgtat cacgatcccc catgctggtc gatagccgtg caaaagcagc gtccaaggcg
      961 ttttctgccc taagcaaaag caggcgggct tcgtccagat tgacctgggc gaagcggacg
     1021 tccaattcgg acttgatttt gttctttgcg aggcttgcaa ttctgtcaaa aatggcagtg
     1081 cgcgcgtcca ccgtcttgat cgcaactcga agcaccgctt gcgcttccag tgccgaaaag
     1141 taagcgtcgc taacttccag aacaacctcc gtacggattg cgtcggcctg gtgctgctgg
     1201 gatacttcgc tgagctgcgc gctttcgact agactcgacg tccgtccgaa gtcggtgacg
     1261 agctggctga gaaagatgcc gttggactct cggcgcaata tgatgggatt gttcaatccc
     1321 ccagctgcga tacgcgtgtc aagtgtaatg tccttaccgc cttgtgtcgt atgttcctga
     1381 tacgcgtccg ctgcagtgat actaccgatc aacagcggat acctagcgga cctgacttga
     1441 attgtgtttt gcttcgcggc cgcgacgttc agattacccg ccttgaccaa cggccggttc
     1501 accagggcca gccgcagtgc ttccgctaaa tttagcggcg cgggttgttg tgcgagtgtg
     1561 cctggcgaag cggcacacat gcaaaccagc aggaacatta gcagaaagcg attagcaggc
     1621 gtcatagtgt tcgatgtaat tccggctctc acgcggactc tggcgaaccg acggggactt
     1681 ccctgcgccg atgcgccagg agataagcgg cagggataat gaacactgtg gtgatcactg
     1741 aaatactcag gccgccaatg atggcgcgag ccaagggagc atacgcctca cttccggtac
     1801 ctaatttcgc cgccatcgga atcaggccga taatggtcgc cagagaagtc atcaacaccg
     1861 ggcgaagcct cacgcggcat gccaatgcga ccgcttcacg caagggtacg ccatccgagc
     1921 gcaggtgccg cgtaaattcc acgatcaaaa tgctgtttga gaccacgatg ccaaccatca
     1981 tgacgactcc cataagtgac atgacgttca gcgtcgtgcc cgtcagcagc aacgttacca
     2041 gcacgccggt caacccagtc gggatggcaa gcagaatcag caacggatca aggaaagacg
     2101 cgaattgagc gaccaggacc agatagacca gtaccacgga cagcagcagt ccgaggccaa
     2161 aactcgaaaa agaagcgcgc atgctctgca ccgtgccgcg cagggccacc cggacgcctt
     2221 cgggcaacac agactgcgag acaatatgct ccacatctga aaccagttcc ctgatatttt
     2281 cttttgcggg cgcaatgtag acgtcgacta cacggcgcaa ctgataatgt ccaacttccg
     2341 tgggtgcccg gataacattc agtttcgcca ttgcatcgag ccggaccgaa tccgtacgat
     2401 tactggcgcg gatcggaatc gctttcaggt ctccaatatt ttcaaccgat tgctctggat
     2461 attgcactgt caacagataa tcgtttccgc tgcgctgatc aacccaatag ctgggcgcga
     2521 tcatcgcgtt cgacgtgagt gccgtgataa tgttgctgac gacttctttc tgggtcaggc
     2581 caagcttggc cgcctgaacg cggtcgatat ccatgcgcag cgacggggca tcgatgtcct
     2641 gcgccaccag tacgtcgttg acgccgggaa gccgccttat ggagtccgca agcttcactg
     2701 cggtatcgtg ggcggcttcc agattcgatc cgcttacctg cacatcgatc ggcgtaggca
     2761 agcccagatt cagcacggca tctaccaggc cgccggattg gaaataaacg gtgatctgcg
     2821 gcagctcgcg cgtaattcgc gcgcgcactt tgtccatgta ttcgtaactc ccgacatcgt
     2881 gctcttcatt caaactgact tgcacgaaag ccgtatgtga cgccgagtta ggcgtataca
     2941 tcgatgagaa ccccggcacc gagccgatgt tggaaacgat caggcgcaaa tccttcgctc
     3001 cgacttcctg ttttacaatc gattcgattt tcgccacggc ttgttcagtc acatcgacgt
     3061 ttgttcccgt gggcgtcttg aggttgatta caaactgtcc gggatcggtc cgggggaaat
     3121 aagccattcc caaaaaaggc agcaatgcca ggctcccgac gaacaagcca agaaacccca
     3181 caagggttac ggtgggatgg cgcagcacgt gccccacggc aacgtcatac agtttcaaca
     3241 tggcctggaa tttctggtt
//
LOCUS       DATMZQ010000249         5273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3298513, whole genome shotgun sequence.
ACCESSION   DATMZQ010000249 DATMZQ010000000
VERSION     DATMZQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298513"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1630
                     /gene="wbaP"
                     /locus_tag="VN496_08105"
     CDS             179..1630
                     /gene="wbaP"
                     /locus_tag="VN496_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HXF16953.1"
                     /translation="MSRARGRLGLNWYLAAGDAVGLGLSWLLALAFLWLIEDQAWKQG
                     IIDWWVTLGYKRTLLFFGLTVLVSLLFGARGHYSRRQPFFDEVLDILVVFIAVAVIDA
                     FIVYLAKWQLSRSWFLTAWIFALALVPITRVTVKRWLIRANHWQRPTVIIGVGTNARE
                     AGKALYGEPLMGLSVIAFLSPPGETLVPTTIEVCGCSIPVRVLDEDPYAILDTLGSPQ
                     IIVALEAESLLPHQKLLQLLSARYSDISIIPPLRGLPLYGMEMTHFFSHEVLMLTVRN
                     NLARPGPRFIKRLFDIIVSLLLLIFLSPLFAFLSWKIRSSGGGAIFGHLRIGQHEKQF
                     HCLKFRTMVENADEVLAQVLERDPDARTEWEQDFKLKNDPRITPIGQFLRETSLDELP
                     QLWNVLKGDMSLVGPRPIIQEELKRYGNQAGYYLEARPGITGLWQISGRNDTGYEDRV
                     ALDCWYVRNWSLWYDLVVLFKTVRVVLKKEGAY"
     gene            complement(1667..2938)
                     /locus_tag="VN496_08110"
     CDS             complement(1667..2938)
                     /locus_tag="VN496_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXF16954.1"
                     /translation="MRILPEARLDVPSVRNAFHALKIEPRSLFILAPAIYLFVLPLAH
                     TTGLRSVAFAVSVLLLVFTWRATPAPSIPLKAAFVAWLTLAVVTLIWAVRPEYSIGEI
                     RAEILQSFLTFIIFFRATQSKRELTIWFVTISASALLVGAFALAHSLRGLNPYHVGLH
                     GGTLYYAGYLNMIFPVLAAMAIVRPGWQRLFLICLMVFLLQTAVGSTSRAVWVGFLIE
                     LAIFGGLYLRYMDVRPVVRRLALTASIAGFVIFCGAFLYVAKQKLQLSGGPSEIIAQA
                     ARADRRPQLWVDSITFIKERPLTGAGFGRMVLGKELVTQQNDPNHTHAHNIILNYALQ
                     LGLFGPIALVFLFYSVVREFWKLTKSSSYELKILGIAGISVLGGIFGQSMVEDIFVRH
                     LAWLFWALAGMALGYAMNNEKSVVHANTVIE"
     gene            complement(2922..3791)
                     /locus_tag="VN496_08115"
     CDS             complement(2922..3791)
                     /locus_tag="VN496_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16955.1"
                     /translation="MRNLTEFSLTEWLRLVPIQHALKQARNDAWLAMYKTIRPKQLSA
                     FLNSNRQLAAQNVALVIAFEQPWALDWLLRMANRNLSDTEVMVFDNSRSEAKRLEIER
                     VCTKNNIPYLALPLNPTRHVNRSHGMAMTWVYYNVVRAINPRVFGFIDHDLIPVRKVN
                     VADKMKDQPIYGLLNVGKFGFWFLWAGYCFFDYAEVKSKSINFLYDFSCDLDTGGRNW
                     NSVYRYLDRSRLRVASSENVSMIIDSVRNSRKVQFIDQNWLHIGGISYSYNSPENIAF
                     FKGLERLFDANPS"
     gene            complement(3825..4244)
                     /locus_tag="VN496_08120"
     CDS             complement(3825..4244)
                     /locus_tag="VN496_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXF16956.1"
                     /translation="MLRKLSAHSPDLAFVLLWSPGALDNPTHPGDDAKAKQVLQQLGG
                     VPVVGFPTPTLSELFAGLSVCERVICSDGGAMHVAAALGKPILCFFGDSDARQWHPWG
                     VPYQLMRSESQDVSDISVSDVLEKFARLQKAVPHIAQ"
     gene            complement(4245..4880)
                     /locus_tag="VN496_08125"
     CDS             complement(4245..4880)
                     /locus_tag="VN496_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16957.1"
                     /translation="MSVTPATARSKILVIRRDNIGDLILTTPLIHALRQRFPEAWIGA
                     LVNSYNARVLDGNPDLDEVFVYTKAKHRGSRSALSAYWSTLSLIIRLRKLRVDYAILA
                     SPAGSSKAQMFARRINARTIVGFGDCADPAVRLKASPAGTLHEVDEVFRIARFFGIEG
                     PPPKTAAFVNKDEIVLARNKIMKVLPANGKLVGIHISARKPKQRWPAERFV"
     gene            complement(4932..>5273)
                     /locus_tag="VN496_08130"
     CDS             complement(4932..>5273)
                     /locus_tag="VN496_08130"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXF16958.1"
                     /translation="DSKVNQGGYSFHQAGSDVEHRVSVEVRDAKSMVQELGIESADLI
                     KIDTEGSEYDILTRLGADFLGKVKWIYGELHGNRDFELLAFLSEQFHIGVRKTMDKRL
                     FMFQARNKTAA"
BASE COUNT         1301 a         1365 c         1360 g         1247 t
ORIGIN      
        1 gatttttctc tgaaggtaaa atgcgttgcg atgtcgcccg ttgttgcagc cataacgcat
       61 ttcgtatgga gcgctggcgc tttctacggt ttcatcaaca gaccgaagcc aaatagaaat
      121 aatgaatgat cgcccaggaa ccgcagtcgg cgtccatgcc ctctcagaaa ccgggcttat
      181 gagtcgcgct cgtggtcgat taggtttgaa ctggtattta gctgccgggg atgcggttgg
      241 acttggcctc agttggttgc ttgctttggc gtttttgtgg ctgatcgaag accaggcctg
      301 gaaacagggg attattgatt ggtgggttac gcttgggtac aagcgcacgc tgctcttttt
      361 tggattgacg gttcttgtaa gtctgctatt cggagcgcgc ggccattact cgcgccgcca
      421 gcctttcttc gatgaggttc tcgacattct tgtggtattc atagcggtcg cagtcatcga
      481 cgcatttatc gtctatttgg cgaaatggca attgtccaga tcctggttcc ttacggcgtg
      541 gatttttgca ctggcgttgg ttcccataac ccgagtgacg gttaaacggt ggctgatacg
      601 cgccaaccac tggcagcggc caactgtcat tattggagtc ggcactaatg cccgagaggc
      661 gggtaaagcc ctctacggtg agccgctaat gggtctttcc gttatcgctt tcctctcacc
      721 cccaggtgaa acactagtgc cgaccaccat cgaggtctgc ggctgttcaa tccccgtgcg
      781 ggttttggac gaagatccgt atgcaatttt ggacacgcta gggtctccgc aaatcattgt
      841 tgctctggag gcggaaagcc ttttaccgca tcaaaagcta ctgcagttgc tgtcagcacg
      901 ttacagcgat atcagcatca ttcccccttt gcggggacta ccgctttacg gcatggagat
      961 gactcacttc ttcagtcatg aagtgctcat gctcacggtg cgcaataatc ttgcgcgtcc
     1021 aggcccgcgt ttcatcaagc gccttttcga cattatcgta tccctattac tccttatttt
     1081 tctctcaccg ctttttgcat ttctgtcatg gaagatacgc agttccgggg gaggcgctat
     1141 cttcggacat ttaagaattg gtcagcacga aaaacaattt cattgtctga aatttcggac
     1201 gatggtggaa aatgcggacg aggtgctcgc gcaagtcctg gaacgcgacc ctgatgcgcg
     1261 aactgaatgg gagcaagatt tcaaactgaa gaatgatccc cgtatcaccc cgattggtca
     1321 gtttctccgg gaaacaagcc ttgatgaatt accgcaattg tggaacgtat taaaaggaga
     1381 catgagcctg gttgggccac gtccaatcat ccaagaggaa ttgaaacgtt atggcaatca
     1441 ggcgggatac tatctggaag cacgtcccgg tattacaggg ctatggcaaa tcagtgggcg
     1501 aaacgatacc ggatatgagg atcgggttgc tctggattgc tggtacgtgc gcaactggtc
     1561 gctatggtac gacctggtgg ttctcttcaa aactgttcga gtggtgctga aaaaagaggg
     1621 cgcatactga acgctccgcc gtgaaaccga agccaccaag caatcgctat tcaatgacgg
     1681 tatttgcatg aactacagat ttctcgttat tcattgcata accaagtgcc attccagcca
     1741 gcgcccaaaa tagccacgcc agatgccgga cgaatatatc ttcaaccatg ctctggccga
     1801 agataccacc gagaacagaa atgccggcaa ttccaagaat cttcagttca tagcttgaag
     1861 acttggttag cttccaaaat tcccgcacaa cggaatagaa aaggaagacc aacgcgatag
     1921 gtccaaacaa tccaagttgc agcgcatagt tcaggataat gttatgcgca tgagtatgat
     1981 tagggtcatt ttgttgcgtc accagttctt ttccaagcac cattcgccca aacccggctc
     2041 cagtcagcgg ccgttcttta ataaacgtaa tgctgtcaac ccatagttgg ggcctgcgat
     2101 cggcccgcgc agcttgggca atgatttcgg acggcccgcc agacaattgc agtttttgtt
     2161 ttgcgacata gaggaacgcg ccgcagaaaa taacaaagcc tgcgatactc gctgtcagcg
     2221 caagtcttct gacaacaggc cggacatcca tatatcgaag gtagaggcct ccaaatatcg
     2281 ccagttctat caggaaacca acccacaccg cacgacttgt tgagccgacg gccgtctgca
     2341 gcagaaaaac cataagacag atcaagaaca acctctgcca gcctggcctg acaattgcca
     2401 ttgcagccag cacggggaag atcatgttga gatagccggc gtaatagagc gtaccgccat
     2461 gcaggccaac gtggtaagga ttcaagccgc gcaacgaatg cgccagcgca aatgcaccga
     2521 caagcagcgc agatgcgctg atggttacaa accaaatagt gagttcacgt ttgctttggg
     2581 ttgccctaaa aaagatgatg aacgtgagaa agctctgcag aatttcggct ctgatttctc
     2641 cgatagagta ttccgggcga actgcccaaa ttaacgtcac tactgccaga gtgagccatg
     2701 cgacgaatgc ggcttttagc gggatagatg gagccggggt cgcacgccaa gtaaaaacga
     2761 gcagcaagac tgaaacggca aatgcaacag agcgcagacc agttgtatga gcaagcggca
     2821 gcacgaacag ataaatggcc ggcgccagaa tgaaaagaga cctgggctca atcttcaacg
     2881 catgaaaggc gttccgcact gaaggtacgt ctaaacgcgc ttcaggaagg attcgcatca
     2941 aacagccttt caagaccttt gaaaaacgca atattctcgg gggagttgta gctatagcta
     3001 attcctccga tgtgcagcca gttctgatca atgaactgca cctttcggct attcctaact
     3061 gagtcgatta tcatgctgac gttttcagac gacgccacgc gcaggcgaga ccgatcaagg
     3121 tagcggtata cgctattcca gttccggccc cccgtatcga gatcgcacga gaaatcgtaa
     3181 agaaagttga tcgatttgct cttgacttcc gcgtagtcaa aaaaacaata gcccgcccac
     3241 aaaaaccaga agccaaattt accaacgttc agcaagccgt atatgggttg atctttcatt
     3301 ttgtcggcca cgttgacctt gcgtacggga ataagatcgt ggtcaataaa tccgaatacg
     3361 cgcgggttta tcgcacggac gacattgtaa taaacccaag tcatcgccat gccgtgcgag
     3421 cgattgacgt gtctggtcgg attgaggggc agcgcaagat atggaatatt gttttttgtg
     3481 cagacccgct caatctcaag tcttttagcc tcgctgcggg aattgtcgaa gaccataacc
     3541 tctgtatcgg acaaatttct gtttgccatc cgcaatagcc aatccaaagc ccatggctgt
     3601 tcgaacgcga taacgagcgc aacattttgc gccgccagct gtcggttgct gttcaggaac
     3661 gcgctcagtt gctttgggcg aatagtcttg tacatagcaa gccacgcgtc gttgcgagct
     3721 tgcttgagcg catgctggat cggaactaat ctcagccact ccgttaagga gaattcagtc
     3781 agattgcgca tgaatcgtcg gcatgagatc tcaagtgaat caaatcattg tgcaatatga
     3841 ggcaccgctt tctgcaatct cgcgaatttt tccagtacgt cggacacaga gatatccgag
     3901 acatcctgcg attcactccg cataagctga tatggcactc cccatggatg ccattggcgg
     3961 gcatccgaat cgccgaagaa gcacaggatg ggctttccaa gcgcggccgc cacgtgcatc
     4021 gctccaccat cgctgcaaat aacccgttcg catacagaaa gtccagcgaa aagctcggat
     4081 agcgttgggg tgggaaagcc gacaaccgga accccgccaa gttgctgcag aacctgtttc
     4141 gccttggcgt cgtcccctgg atgggtggga ttatcaagcg cgcccggaga ccacaacaaa
     4201 acaaatgcga gatccggact gtgtgcggaa agtttgcgaa gcaattacac gaatctttcg
     4261 gccggccacc gctgttttgg tttgcgtgca ctgatgtgta tgccgaccag tttgccgttt
     4321 gccggtagca ctttcattat cttattgcgg gccaaaacta tttcgtcctt gttgacgaaa
     4381 gcggccgttt ttggcggcgg gccctctatt ccgaaaaacc gcgcgatgcg gaaaacctca
     4441 tcgacctcgt gcagggtgcc agccggactc gccttaagtc tgactgcggg atcggcgcag
     4501 tcgccgaaac cgacaatggt gcgcgcatta atccggcggg cgaacatctg ggcctttgaa
     4561 gacccggccg gcgaagcgag aatcgcataa tcaacgcgca gctttcttag ccggatgatc
     4621 aggctcagcg tcgaccaata cgctgacaat gcgcttctgg atccccggtg ttttgccttt
     4681 gtatagacga acacttcgtc aagatcagga ttcccgtcga gcactctggc gttgtagctg
     4741 tttaccagcg cgccgatcca cgcttcaggg aaacgctggc gaagcgcgtg gattaagggg
     4801 gtcgtgagga tcaaatcacc gatgttgtca cggcgtatga cgagaatctt cgagcgcgct
     4861 gtcgcaggtg tgacgctcat gctctgcgtt tcggcgaaaa cagtggtcgc gacaggaaaa
     4921 agtgaaggga tctaggcagc agtcttgttc cgcgcctgga acatgaacaa ccgcttgtcc
     4981 atggtctttc tcactccgat gtggaactgt tcactcagaa aggcaagcag ctcgaaatcc
     5041 cggttgccgt gcaactctcc ataaatccac ttcaccttgc ccagaaagtc agcccctagc
     5101 ctggtcaata tgtcgtattc agagccttca gtatcgatct tgatcaagtc cgcggattcg
     5161 attccgagct cttgtaccat ggatttcgcg tcgcgtactt caacactcac ccggtgttca
     5221 acatcactac cggcttgatg gaaggaatag cccccttgat tcacttttga atc
//
LOCUS       DATMZQ010000250         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1481066, whole genome shotgun sequence.
ACCESSION   DATMZQ010000250 DATMZQ010000000
VERSION     DATMZQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481066"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="VN496_08135"
     CDS             <1..1597
                     /locus_tag="VN496_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HXF16959.1"
                     /translation="RFPPEPNGYLHYGHAKSICLNFGLALQYGGRCHMRFDDTNPVTE
                     ETEYVESILDAVKWLGFDWGEHLYYASDYFDTLYDFAEAFIEHGLAYVDSLTADEMRE
                     YRGTLTEAGHSSPYRDRSRQENLDLFRRMKAGEWPDGAHVLRLKIDMSSPNINLRDPV
                     IYRIRHAHHHRTGDKWCIYPLYDYTHCISDALENITHSLCTLEFEDHRPLYDWVLEQL
                     AEFGLLARPLPHQYEFARLNLSYVVLSKRKLIQLVEEKHVDGWDDPRMPTIVGARRRG
                     FTPEGFRLFIDRIGVSKSDSWIDMSVLEDCMRDTLNETAERRMVVLDPVKLVIDNYAE
                     DKEETCEAPNHPQKPERGKRDLAFSRELWIEREDYMEVPAKGYFRLFPGNQVRLRYGF
                     VLKCIGADKDAKGTITAVHCEMLPDSKSGTPGADVYKVKGNIHWVSARHAYQANVRLY
                     DRLFAVPHPGAERDFLADLNAASKTIARAYCEMSLKQAKPEDRFQFERHGYFCADRGD
                     SRPNAPVFNRTVTLRDSWGKSPT"
     gene            1594..2412
                     /locus_tag="VN496_08140"
     CDS             1594..2412
                     /locus_tag="VN496_08140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXF16960.1"
                     /translation="MKTVSHTAERPRTWHPGIAIWVTGAFHIVAIAALLARPQWWPSI
                     LGLVILNHVALGLIGLWPRSRLLGSNMLRLPDAAVKRGEIAVTFDDGPDPDVTPKVLD
                     ILDAHRAKASFFVIAERAAAHPALVREIVRRGHSIENHSRRHASCFALFGWSALKQDV
                     GAAQEIIERITGRSPAFFRAPMGLRNPMLDPLMAIMGLRYTAWTRRGFDTVTRDPAVV
                     LRRLTRNLSAGDILLLHDRRCRDDQPIALEVLPPLLEQIEARGLRPVSLPLAMQ"
     gene            2412..3116
                     /locus_tag="VN496_08145"
     CDS             2412..3116
                     /locus_tag="VN496_08145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF16961.1"
                     /translation="MQSQTTLIEAVSRPYLAAGHWPYYFARAKLTHDPIYLTLLRSGR
                     FSDRARVLDLGCGHAFLAALMLTARKHYESGLWRADWIAPPADLQFIGIDSQTRVVTQ
                     AKVVLGDRVVMHAADLREAPLPQADVAVAIDVLQCLKADVQVALLKKIARSLSGGGLL
                     VLRVPDAGGGWRARFGKASDRFGIPLTKRAFATYHHRTLGEWLQLLQTLGFEPSVDPS
                     RQTFANTVLWAKAPQA"
     gene            3171..>3354
                     /locus_tag="VN496_08150"
     CDS             3171..>3354
                     /locus_tag="VN496_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16962.1"
                     /translation="MHEIELADFRNRRVAIWVQNRWLVYVCLVLCLSLLILAGALNVP
                     AQLAWTAGTLACGLIAF"
BASE COUNT          674 a          967 c          987 g          726 t
ORIGIN      
        1 tcgcttcccg ccagaaccaa atggctatct gcactacggg catgccaaat caatctgcct
       61 gaattttgga ctggcattgc agtacggggg gcgctgccat atgcgtttcg acgataccaa
      121 ccccgttacc gaagagaccg aatacgtcga atccatactg gatgcggtca aatggctggg
      181 cttcgactgg ggtgaacatc tttattacgc atcggattac ttcgatacgc tgtacgattt
      241 cgccgaggcg ttcatcgagc acggcctggc ttatgtggac agcctgaccg cggacgaaat
      301 gcgcgaatat cgcggtacgc tcaccgaggc cggacattca agtccctatc gcgatcgctc
      361 gcgccaggaa aaccttgatc tgttccggcg catgaaagcg ggcgaatggc cggatggtgc
      421 gcacgtgctg cggctgaaga tcgacatgtc atcgccgaat atcaacttgc gcgatcccgt
      481 catctaccgc atccggcatg cacatcacca tcgcacaggt gacaaatggt gcatttatcc
      541 gctgtatgac tacacgcact gcatttcgga tgcgctggaa aacatcacgc attcgctgtg
      601 cacgctagag ttcgaagacc accgcccgct ctacgactgg gtgttggaac aactggcgga
      661 attcgggctg ctcgccaggc cgctgccgca tcagtacgaa ttcgcccggt tgaatctatc
      721 ctacgtggtg ctctccaagc gcaaactcat ccagctggtc gaagaaaagc acgttgacgg
      781 ctgggatgac cctcgcatgc ccacgatcgt cggcgcgcgt cgtcgcggct tcactccgga
      841 gggattccgt ctgttcattg accgcatcgg ggtatctaaa tccgattcgt ggatcgatat
      901 gagcgtcctg gaagattgca tgcgggacac gctgaacgag acggcagaac ggcgcatggt
      961 ggtcctcgat cccgtcaagc tggtcattga caattacgca gaggacaagg aggaaacgtg
     1021 cgaagcgcca aaccatccgc aaaagcccga gcggggcaaa cgcgatctcg cgttctcgcg
     1081 tgaactctgg attgagcgcg aggactacat ggaagtgcct gccaagggtt atttccgttt
     1141 gttcccgggc aaccaggtac ggcttcgcta tggattcgtg ctcaaatgca ttggtgcaga
     1201 caaggatgcc aagggcacca ttacggccgt gcactgtgag atgctgccag actcgaagtc
     1261 cggcacgcct ggcgcagatg tctacaaagt caaaggtaac atccattggg tatcggcgcg
     1321 ccacgcatac caggccaatg tgcgactcta tgaccgtttg tttgctgtcc cgcatccagg
     1381 agcggagcgc gactttttgg cagatctgaa tgccgcttcc aaaacaattg cgcgcgcgta
     1441 ttgtgaaatg tcacttaaac aagccaagcc cgaggatcga ttccagttcg agcgtcacgg
     1501 ctatttttgc gcggatcgcg gcgactcacg ccccaatgcg ccagtattca atcgcaccgt
     1561 gacgctgcgc gattcgtggg ggaagtcgcc aacgtgaaaa ctgtttcgca tacggcggaa
     1621 cgtccccgaa cctggcatcc cggcatcgcc atctgggtca cgggtgcatt tcatattgtc
     1681 gccattgcgg cgctgctcgc gcggccacag tggtggccgt caatactcgg gctcgtcatc
     1741 ctgaatcatg tcgcgctcgg gctaattggg ttatggccgc gaagccgcct gctgggcagc
     1801 aatatgctgc ggctgccaga cgctgcggtg aagcgcgggg aaatagcagt gaccttcgac
     1861 gatggtccgg acccggatgt tacgccgaag gtattggaca ttctggatgc ccatcgggcg
     1921 aaagccagct tctttgtcat cgccgagaga gccgccgcgc atcccgcgct ggtgcgtgaa
     1981 atcgtccgcc gcgggcacag catcgagaac catagccgca gacacgcaag ctgcttcgcg
     2041 ctgttcggct ggtccgcgct caagcaggat gtcggagcgg cgcaggaaat tatcgagcgc
     2101 attaccggac gctcgcctgc tttctttcgc gcccccatgg gactacgtaa tccgatgctc
     2161 gatccgctaa tggcaatcat gggactgcgc tatacggcat ggacgcgccg tggcttcgac
     2221 acagtcacgc gggatccggc agtagtgttg cggcgcctta cccgcaatct atctgcggga
     2281 gatattcttt tgcttcatga ccggcgctgt cgcgacgacc aaccgattgc gctggaggtc
     2341 ctgccaccgc tgctcgagca aatcgaagcg agagggttgc ggccggtatc cctgccgctc
     2401 gccatgcagt aatgcaatcc caaaccacgc tgatcgaggc tgtgtcccgg ccttatcttg
     2461 cggccgggca ttggccgtat tattttgcgc gcgcaaagct aactcacgat cccatatacc
     2521 tcacattgct gcgctcgggt cgtttttcgg atcgagctcg ggtacttgat ctgggttgcg
     2581 ggcacgcgtt tctggccgcg ctcatgctga cggcgcgcaa gcattatgag tcgggcctgt
     2641 ggcgcgccga ctggatagcg ccaccggccg atttacaatt catcggcatt gactcgcaga
     2701 cccgcgtggt cacacaggcg aaagttgtac tcggcgatcg cgtggttatg catgcggcgg
     2761 atttacgcga ggcgccgttg ccgcaggccg atgtagccgt cgcaatcgat gtactgcagt
     2821 gcctgaaagc ggacgttcag gttgcattgc tcaaaaaaat cgcacgctcg ttgtccgggg
     2881 gtggattact cgtgctgcgc gtgccggatg ctggaggggg atggcgcgct cgttttggca
     2941 aggcttccga tcggttcggt attccattga ccaagcgcgc attcgcgacc tatcaccatc
     3001 ggacgctcgg cgagtggttg caactgcttc agacgctggg atttgagcct agcgtagatc
     3061 cgtcacggca aaccttcgcc aatacggtgc tgtgggcaaa ggcgccccaa gcttgagcat
     3121 cgacaaccaa cgccgcacaa tgccatcttg tcgcagtgta gggtttcggc atgcacgaaa
     3181 tcgagctagc ggattttagg aatcgccgcg tcgccatctg ggttcaaaac cgctggctgg
     3241 tatacgtttg cctggtcctc tgcctttcgt tgttgattct cgccggtgct ttgaacgtgc
     3301 cggcgcaatt ggcatggacc gcgggtacgc tggcctgtgg tcttatcgca ttta
//
LOCUS       DATMZQ010000251         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3995004, whole genome shotgun sequence.
ACCESSION   DATMZQ010000251 DATMZQ010000000
VERSION     DATMZQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3995004"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            651..>2012
                     /locus_tag="VN496_08155"
     CDS             651..>2012
                     /locus_tag="VN496_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXF16963.1"
                     /translation="MYDALLDELEDDGAPHQEEVADLTDKPAGDEDVDDELDSPDLVT
                     DLDLDIDPKADDLLAVVDEEAESWSDDPVRMYLTQMGEIPLLTRQEEIALAKRIEITR
                     ANFRRKLLECDYVVQDAVKVLRRVHAGELPFDRTVQVSVTDHLEKDQILGRFPHNLRT
                     VEILLKQNKDDFRIATSKSYKMTQRRDAWQRLGRRRLRAVKLVEELGLRTQRLEPKIK
                     ALEEFSRRVDTLKAQIEAHRKAKGSLNERKPWIMEYRNCLRATQETPRSLRHRVQYLK
                     VVYSEYQKAKRGLSEGNLRLVVSIAKKYRNRGLSFLDLIQEGNAGLMRAVDKFEYRRG
                     FKFCTYATWWIRQAITRAVADQSRTIRIPVHMVETMSRVRNVSRELLQELGREPTIEE
                     TAKRAGTAIDEARRVLAMSRYPISLDRPVGNSEDSHFGDLLPDSGAENPAVGAAQEML
                     RGRI"
BASE COUNT          443 a          555 c          625 g          389 t
ORIGIN      
        1 tgataacaca gcggttgggc gccacaaacc ggcgcgatcg aagcgtgcgt tcgagcattc
       61 tgctcgacct aattcccgcg cgagcgcgcg gggtttcgtt tcatgactcc ggcgaagcaa
      121 acgcgactgc gcgcagtgtt agcacgaatt ggacgaaaag ttgcgcgaac ttttggcgca
      181 aacgctggac cgaaagcatt gcgtgagtag aattggggcg cacgaaaact ggcaccatgc
      241 cccttgaaag gggagcgata catccaacac gagacgtggt tcggacgcaa gtgtccagtc
      301 agtgcgcaga gccgtatttg ttgaaatgga taagataggt gatatggggt gatagctgtc
      361 gtttgctgcg tgccctaatc tcttaccaaa tgtcggacag cggcggcact ttaataaatc
      421 ggtgatagaa tttgttggct ttcatcggta gttctgggga taatggccgt tgactttaac
      481 cggtgttgca ccaatcctcg tggcactggc aggaggcatt gtacctacct tggcccctgc
      541 gacacgcaca tccaatggtg tcaacctttc ccggtggctc ccgctgattt ccccgccgaa
      601 tcacgagtgg ttcaaaccac acgaactaag ctcacttcag gagtgttgac ttgtacgacg
      661 cattattgga cgaattggaa gacgacggtg ccccgcatca ggaagaagtg gccgacctga
      721 cggacaagcc ggctggcgat gaggacgttg atgacgaact cgattcgccg gatctggtaa
      781 ccgatctcga cctggacatc gacccaaagg ccgacgacct attggctgtg gtcgacgaag
      841 aggccgagag ctggtcggat gacccggtgc gcatgtatct gacgcagatg ggcgagattc
      901 ctctgctcac gcgtcaggaa gaaatcgcgt tggccaagcg gattgaaatc acgcgggcca
      961 atttccgtcg taagctgctg gaatgtgact acgtggtgca ggatgccgtg aaggtcctgc
     1021 ggcgcgtgca cgccggcgag cttcccttcg atcggaccgt tcaagtttcg gtaaccgatc
     1081 acctcgaaaa agaccagatt ctcggccgtt ttccgcataa tttgcgcacg gtggaaatcc
     1141 tgctcaagca gaacaaagac gacttccgca ttgcgaccag taagtcctat aagatgaccc
     1201 agcgccgcga cgcctggcag cggctgggtc gccggcgact gcgggccgtc aagctggtcg
     1261 aagagttggg tctgcgcaca cagcggctcg agcccaagat caaggcgctc gaagagttca
     1321 gccgccgtgt cgacacgctc aaggctcaga tcgaggccca tcgcaaggcc aagggatcgc
     1381 tcaacgagcg caagccctgg atcatggagt accgcaactg tttgcgggca acgcaagaaa
     1441 cgccgcgcag cctgcgccat cgcgtgcagt atctgaaggt cgtttacagt gaatatcaaa
     1501 aggccaagcg cggtttgtcg gaaggcaacc tgcgattggt cgtgtcgatc gccaagaagt
     1561 atcgcaaccg tggcttgagc ttcctggatc tgattcagga aggcaacgcc ggcctgatgc
     1621 gggcggtcga caagttcgag tatcgacgcg ggttcaagtt ctgcacgtat gccacctggt
     1681 ggattcgtca ggccattacc cgcgccgtgg cggaccagag ccgcacgatt cgcattccgg
     1741 tgcacatggt cgagaccatg tcccgcgtgc gcaacgtgtc gcgcgagctg ctgcaagagc
     1801 tgggacgcga gccgacgatc gaagagacgg ccaagcgcgc cggcacggca atcgacgagg
     1861 cgcgtcgcgt gctggccatg agccggtatc cgatcagcct ggaccggccg gtcggaaaca
     1921 gcgaggacag ccattttggc gatcttctgc ccgacagtgg ggccgagaat ccggcggtgg
     1981 gcgccgctca ggagatgctc cgcggtcgga tc
//
LOCUS       DATMZQ010000252         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2614312, whole genome shotgun sequence.
ACCESSION   DATMZQ010000252 DATMZQ010000000
VERSION     DATMZQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2614312"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VN496_08160"
     CDS             <1..798
                     /locus_tag="VN496_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF16964.1"
                     /translation="LAQFETRSGPEFTMRYNEYRSAQINASAAPGYSSDQASAALEDV
                     FRQTMPREMGFDYMGMSYQEQKARQGVPPSVIFGFSLLFVFLILAALYESWTVPFSVL
                     LSTPVAVFGALAALWLRRVVVGLYLPQYMVQIENDVYAQIGLVMLIGLAAKNSILIVA
                     FAKEDYERGMSIVDAALSAARLRFRPLMMTALAFILGCVPLWTASGAGSVARQIMGTA
                     VIGGMVAETFIGRFFVPAIFYVVEKLSGAERRRALPLTPAEAPSQGD"
     gene            801..2198
                     /locus_tag="VN496_08165"
     CDS             801..2198
                     /locus_tag="VN496_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXF16965.1"
                     /translation="MKNTTPLGILAVLILEAGCMMGPKYKRPTVDVPQEYRAHAPQQA
                     AQASSLGNEGWWQVYQDPVLAQLIHTAIADNYDVRIAAARVLEAQAQVGITRSNQFPS
                     ASVGADVFSQQNAKVTKLFPAYQVNGGELNLAVIWNLDFWGKYRRQTEAARAQLLATE
                     WGQRAVLSSLVANVATAYFQLRALDSELELSKRTLASRQQSLKLTQVLESHGSASGLD
                     VSQAEQLVYTASETIPDLERQIQQQENLLSVLLGENPQSIPRGRALTEQPVPQNVPAG
                     LPSELLERRPDVRQAEENVVAANAQIGVAKAAFFPNLSLTGLGGLESNALHQFISQPS
                     EIWSGAFSVSQPVFQGGALHSQLRLARANRQETVFSYQQTVQNALEQVSNSLVASQKN
                     REFREQQELLTRAAQQTDQLSEVLYKNGGASYLQVLTSESNFFSAELNLVQAQLNERL
                     ALVQLYQAVGGGWQQ"
     gene            2296..2410
                     /locus_tag="VN496_08170"
                     /pseudo
     CDS             2296..2410
                     /locus_tag="VN496_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394698.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            2518..2964
                     /locus_tag="VN496_08175"
     CDS             2518..2964
                     /locus_tag="VN496_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXF16966.1"
                     /translation="MTDDADARTVQALRSDLALQIARHIRCNGQTQVAAAKRLAVPQP
                     TVSQIMHGKVSQLSLELLIRIAVRAGLPVVLQTGKDAEEAGAFVAGEPLHESRILRSK
                     VADDARQALNEDARNMTPQQRLEAHARHSQLMVELRRAGRKSRRAR"
     gene            2961..>3451
                     /locus_tag="VN496_08180"
     CDS             2961..>3451
                     /locus_tag="VN496_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16967.1"
                     /translation="MKARGAGQSAHLLLNVSAVLAENDVEYAVIGAMAAAVYGAIRAT
                     TDADALLSVSVARLRQLEKVFKKAGFTTEVRRGDADDPIPALLSVSDRHGNRVDLLAG
                     LRGLDQLALSRSITVPFLGSSLRVIGREDFIAMKCFAGGPQDIADAVQALKTVDQPLD
                     LEL"
BASE COUNT          716 a         1013 c         1009 g          713 t
ORIGIN      
        1 ctggcacagt ttgaaacccg ttccggtccc gaatttacga tgcgatacaa cgagtatcgg
       61 tccgcgcaaa tcaacgctag cgccgcgccg ggttatagct cggatcaggc atcggcggcc
      121 ttggaagatg tcttcaggca aactatgcct cgcgaaatgg gctttgacta catgggcatg
      181 tcttaccagg agcaaaaggc gcgacaagga gtgccgccgt ccgtcatctt cggattttcg
      241 ctgctgttcg ttttcctgat tctggcagcg ctctatgaaa gctggacagt gccttttagc
      301 gtgctgctca gcacgccagt cgcagtcttc ggcgctctgg ctgctctctg gctgcgtcgt
      361 gtcgtagtcg gcctttacct gccgcagtac atggttcaaa tcgagaacga tgtgtacgca
      421 cagattggct tggtgatgct gatcggcctg gctgccaaga actcgatttt gatcgtggca
      481 tttgccaaag aggactacga acgcggcatg tccatcgtcg atgccgcact atccgcggcg
      541 aggcttcgct tccggcccct catgatgacc gcgctcgcct ttattctcgg ctgcgtgccg
      601 ctctggacag cgtccggagc aggctcggtc gcgcgccaga ttatgggcac ggcggtaatt
      661 ggcggcatgg tggcggagac cttcatcggc agattctttg ttcctgcaat tttctacgta
      721 gttgagaaat tgtcaggcgc cgagcgacgg agagcattgc cgctcacgcc cgccgaggcg
      781 cccagtcaag gggactaatt atgaaaaaca ctacaccctt aggaattctg gctgtgctga
      841 ttctggaagc tggctgcatg atgggaccga agtacaagcg gccgactgtg gacgtcccgc
      901 aggaatatcg tgcacacgcg ccgcaacagg ccgcccaagc ctcgtcgttg gggaatgaag
      961 gatggtggca ggtctaccag gatccagttc tggcacaact gatccacact gccattgcgg
     1021 acaactatga cgtgcgcatc gctgctgccc gcgttcttga agcccaggcg caagtcggga
     1081 ttacgcgctc caaccagttc ccgtcggcga gcgtcggggc ggatgtcttc agccagcaaa
     1141 acgctaaggt gacaaagcta tttcccgcat atcaggtcaa tggcggtgaa ctgaatctcg
     1201 cggtcatctg gaatctggac ttctggggga aataccgccg tcaaaccgag gcggctcgtg
     1261 cccaactact cgcaaccgaa tggggacagc gagcggtgct gtcttcgcta gtcgctaatg
     1321 tagcgacggc gtatttccag cttcgcgcac tcgacagcga actcgaactc tcgaagcgca
     1381 cgctggcatc ccgacaacaa tcccttaagt taactcaggt gcttgagagc catggctcgg
     1441 cctctggact tgacgtaagt caagcggagc agctcgtcta cacggcttcg gagacaatcc
     1501 cggatcttga gcgccaaatt cagcagcagg aaaacctgct cagcgttctt ctcggcgaaa
     1561 atccccaatc cattccccgc ggacgcgcgc ttaccgaaca gcccgtcccc cagaacgttc
     1621 cggccggcct tccctcggaa ttactcgagc gccggccgga cgtccgccaa gcggaagaaa
     1681 acgtggttgc ggccaacgca caaatcggcg tcgcaaaagc cgcgttcttt ccaaacctgt
     1741 cgctcactgg actaggaggg ctggagagca acgccttgca tcagttcatc agccagcctt
     1801 cggagatatg gtccggggcc ttcagcgtga gtcagcccgt ctttcagggc ggcgccctcc
     1861 actctcaact caggctcgca cgcgccaacc ggcaagaaac agtgttctct taccagcaaa
     1921 ccgtccagaa cgcccttgaa caagtttcca attcactggt tgcttctcaa aaaaaccgtg
     1981 agttccggga gcaacaggaa ttgctcacac gagcggcgca gcagaccgac caactgtccg
     2041 aggttctcta taagaacggc ggcgcgagtt atctccaggt gctgaccagc gaatcaaatt
     2101 tcttctcagc ggaactaaat ctggtgcaag cccagctcaa cgagcgattg gcgctcgtgc
     2161 agctttacca ggccgttggc ggtggctggc agcaataagg gaaaggtaaa gatgttcaag
     2221 agaattgcgg ttgcgcacga cggatctcct gaatctggat ctgcattatg aaacggatag
     2281 cgtttctttc aattctccgg tggcgtaatg cttcctaatt acgcgccgtt gtcggtcgtc
     2341 gagcagttcg gcctgctcga gcagctgcac ccccggtcgg attgacctgg gcttgggccg
     2401 gtcgcccggc ctcagctgtt cccccgagat tgcaatctcg cttttctcac accgagagaa
     2461 ccgcaaagga ctccgtccct tgcatagtat attcgctaat gttatagtta gtacactatg
     2521 accgatgatg ccgatgcccg cactgtccag gcgctgcgca gcgatctggc gctgcagatc
     2581 gcgcgccaca tcaggtgtaa cggccaaacc caggtcgccg cggcgaagcg gttggctgtc
     2641 ccacagccga ctgtgagcca gatcatgcac ggcaaagtct ctcagctttc cctcgagctg
     2701 ctgatccgca ttgcagtccg tgccggattg ccggtggtgc tgcagacagg caaggatgcg
     2761 gaagaggcag gagcgttcgt cgcgggcgag ccactgcacg agagtcggat cctgaggtcg
     2821 aaggttgccg atgacgcacg tcaggcgttg aacgaggatg cgagaaacat gaccccgcag
     2881 caacggctcg aagcgcatgc ccgacacagc cagctgatgg tcgagcttcg ccgggcgggc
     2941 cgaaagagca ggagggctcg atgaaagcgc gcggggcagg tcaatctgcg catctgttgc
     3001 tcaatgtaag cgcagtgctg gcagaaaacg acgtcgagta cgccgtcatc ggtgctatgg
     3061 ccgccgcggt gtatggcgcc atacgtgcaa cgacggatgc ggatgccctg ctatccgttt
     3121 ccgtcgccag gctcaggcag ctcgaaaaag tattcaagaa agcgggattt acaacggagg
     3181 tgcgtcgcgg ggatgcggac gatcccattc ccgcgctgct ctcagtaagc gaccggcacg
     3241 gcaatcgggt ggatctgctg gcgggccttc gcggtctcga ccaactggcc ttgtcgcgct
     3301 cgatcaccgt gccctttctc ggcagttccc tgcgtgtgat tgggcgggag gactttatcg
     3361 ccatgaagtg cttcgccggc gggcctcaag atatcgccga tgcggtccaa gcgcttaaga
     3421 ccgtcgatca gccccttgac ttggagctgt t
//
LOCUS       DATMZQ010000253         2779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3554018, whole genome shotgun sequence.
ACCESSION   DATMZQ010000253 DATMZQ010000000
VERSION     DATMZQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554018"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..826
                     /gene="rfbD"
                     /locus_tag="VN496_08185"
     CDS             5..826
                     /gene="rfbD"
                     /locus_tag="VN496_08185"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HXF16968.1"
                     /translation="MFGDVVAPARNVMDLASADSIIAAVRSVRPNLIVNAAAYTAVDK
                     AESEPELAMKVNGEAPRILAEQAALHGAALIHYSTDYVFDGRKEQPYREEDLTAPINT
                     YGRTKLAGERGVAGSGAAHLVFRTSWVYGPRGHNFLLTMLKLGQERKEIKVVNDQFGA
                     PTSARLIAEATACVVAKTLSRGKLDLNALQNASGLYHLTASGHTTWHGFAQAILLGKS
                     GMAKVVPIPTSAYPTPARRPQNSLLDNAKLERQFGFELQDWKVGLELCLEEINDR"
     gene            complement(841..2253)
                     /locus_tag="VN496_08190"
     CDS             complement(841..2253)
                     /locus_tag="VN496_08190"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HXF16969.1"
                     /translation="MIFPVVLSGGSGSRLWPLSRTLLPKQFLPLVGEKTMLQDTLLRL
                     DGIAGLKKPVLVCSNDHRFLAAEQLREISIVAGAQILEPAGRNTAPAVAVAALHVVTE
                     DSDGIMLVLPADHLIRDIGQFHTVVGKAAAAAANGRLVTFGIVGREPETGYGYIERGQ
                     GLEKSTDCYRVARFVEKPDEATARQFVESGRFYWNSGMFVFKASRYLEELGKYRKDIL
                     VAAKAAWAAATRDLDFVRLDEKAFIACPSDSIDYAVMEKTGDAVVVEADIGWSDIGSW
                     TALSQAGKPDASGNVTLGDIHTDNVRNCYVRAEHRLVAAVGVQDLIIVETSDAVLVTH
                     KDHSQEVKKIVDALKAKQRDEYLVHKRVYRPWGYYEGLDSSDRFQVKRIMVKPGCKLS
                     LQMHHHRAEHWVVVSGTAKVIRGDDTLLLSENQSTYIPLGAKHRLENVGKMALHMIEV
                     QSGSYLGEDDIVRLEDDYKR"
     gene            2412..>2779
                     /locus_tag="VN496_08195"
     CDS             2412..>2779
                     /locus_tag="VN496_08195"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXF16970.1"
                     /translation="MAKVALITGVTGQDGAYLAEYLLHKGYEVHGIKRRTSLFNTDRI
                     DHLYQDPHIEHRNFILHYGDMTDSSSLVHVIQKTQPDEIYNLAAQSHVAVSFEEPEYT
                     ANSDALGTLRILEAIRILGLG"
BASE COUNT          628 a          788 c          743 g          620 t
ORIGIN      
        1 ggccatattt ggtgatgttg tagcgccggc gcgaaatgtc atggaccttg cttcagcgga
       61 ttccatcatc gctgcagtaa ggagcgtgcg tccgaacctg attgtcaatg cggcggctta
      121 taccgcagta gataaggcgg aatcagaacc agagttggcc atgaaggtaa acggtgaggc
      181 gccgcgcatt ctcgcggaac aggctgcctt gcatggcgcc gcattgattc attactcgac
      241 tgactatgta ttcgatggcc gcaaggaaca gccttatcgc gaagaagact tgacggcgcc
      301 aatcaacaca tacggccgta ccaaacttgc tggcgagcgc ggtgttgctg gctcgggtgc
      361 ggcccatctc gttttcagaa ccagctgggt atacggaccc cgcggccata attttctatt
      421 gacgatgttg aagctcggcc aggaacgcaa ggaaataaaa gtcgtaaacg accagtttgg
      481 cgcgccaaca tctgcgcgac tgattgccga agcgactgcc tgcgtggtcg caaaaacctt
      541 gtctcggggc aagctggacc taaatgcgct gcagaatgcc agtggcctct atcacctcac
      601 tgcatcgggg catacaacgt ggcacgggtt tgcgcaggcg attttactgg gaaagtccgg
      661 tatggctaag gtggtcccga tccccacgtc cgcttatccc acaccagccc ggcgtccgca
      721 gaattcgctg ctggataacg ccaagctgga aaggcagttc ggctttgagc tacaggactg
      781 gaaggtcgga ttagaattgt gtctggagga gattaatgac cggtgaccgt ttaccgctcg
      841 ctatcgttta tagtcgtctt ccaaccgcac gatgtcatcc tcgcccagat agctgccgga
      901 ttgcacttca atcatgtgaa gtgccatctt gccgacgttc tccagccggt gtttcgcgcc
      961 aagcgggatg taagtcgact gattttcaga tagcagcaat gtgtcatctc cgcggataac
     1021 cttcgcggtt ccggaaacga cgacccagtg ttctgcacgg tgatgatgca tctgcagtga
     1081 aagcttgcac cccggcttaa ccatgatgcg tttcacctga aaacgatcac tgctatcgag
     1141 gccttcgtag taaccccatg gacggtaaac gcgcttgtgc acgaggtact catcgcgctg
     1201 cttggccttc agcgcatcga cgatcttttt gacctcctgc gaatggtcct tgtgcgtaac
     1261 cagtactgca tcagacgtct caacgatgat gaggtcttgc accccgactg ccgccaccag
     1321 ccggtgctcc gcacgcacat agcaattgcg cacgttatcg gtatggatgt ctccaagggt
     1381 gacgttgccg cttgcatccg gtttgcccgc ctgcgacagc gcggtccatg aaccaatatc
     1441 actccaacca atatccgcct ccactacgac ggcatcgccg gtcttttcca tgacggcgta
     1501 gtcgatggag tcggaaggac aagcaataaa ggccttttca tcgaggcgaa cgaaatcaag
     1561 atcgcgtgtg gccgcggccc aagctgcctt ggccgccaca agaatatcct tgcggtactt
     1621 gcccagttct tccagataac gcgatgcctt gaatacgaac attccgctat tccagtagaa
     1681 ccggccagat tcaacaaact gacgtgccgt ggcctcatcc ggcttttcga cgaaacgagc
     1741 cacgcgatag caatcagtgg atttttccag cccctgcccg cgctcgatat aaccgtaccc
     1801 cgtttcaggt tcccgaccga caatgccaaa agtaaccagc cggccattgg ccgcagctgc
     1861 ggccgctttg ccgaccactg tgtggaattg gccaatatcg cgaatcagat gatctgccgg
     1921 caataccagc atgattccat ccgaatcctc ggttacaaca tgcagtgcgg cgacggccac
     1981 tgcgggtgcg gtattgcggc cggcaggctc aaggatctgt gctcccgcca ctatgctgat
     2041 ctcgcgaagt tgctcggccg caagaaaacg gtggtcattg ctgcagacca gaaccggctt
     2101 tttcaaaccc gcgatgccgt caagacgcag caatgtatcc tgcagcatgg tcttttcgcc
     2161 taccagcggc agaaactgct ttggcagcag tgtgcgcgac agcggccaca gcctgctgcc
     2221 gctgccacct gaaagaacaa ctggaaaaat catgcggtgc ctcgtcctgg caagaaccta
     2281 tactggcttt tgtttccgcg tgaatacaaa tcgcttaaaa tcgcagacat tccagcctgg
     2341 ttattttcga tgaacgcacg ctttgcggta agcgcatttg aagtatacag aaccggtgtc
     2401 gttcctgact gatggctaaa gttgccttga tcaccggagt gaccggtcag gacggcgcct
     2461 accttgcgga atacctgctg cacaagggtt atgaagttca cggcatcaag cgccgcacct
     2521 cgttgttcaa tacggaccgg atagatcatc tgtatcagga tccgcacatt gaacatcgca
     2581 attttattct tcattacggg gacatgacgg actcgagcag cctggtgcat gtgatccaga
     2641 agacgcagcc ggatgaaatc tacaatctgg cggcgcagag tcatgtcgca gtgtcgtttg
     2701 aagagccgga gtacacggcc aattcagatg cactcggcac gcttcgcatc ctggaagcta
     2761 ttcgcattct cggcctggg
//
LOCUS       DATMZQ010000254        13942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2962344, whole genome shotgun sequence.
ACCESSION   DATMZQ010000254 DATMZQ010000000
VERSION     DATMZQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13942
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962344"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..2264
                     /locus_tag="VN496_08200"
     CDS             111..2264
                     /locus_tag="VN496_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HXF16971.1"
                     /translation="MEKSFKKEIGLLSLGAGQIFYGEGILAITKALLQSGVAYVGGYQ
                     GAPVSHLIDVMVQGREYLNTLGVHVEACSNEASAAAMLGASIHYPLRGAVTWKSIVGT
                     NVAADALSNLSSPGVTGGALIVVGEDYGEGASVIQERTHAYALKSSMLMLDPRPELSN
                     MVRMVEQGFQLSEASNMPALMELRIRACHVRGSFVCKDNIAPAISTRHLMAEPAKFDY
                     ARLAHPPVTFRHEKLKVDDRIPAARRYIAENQLNELMAGARAEAGFIVQGGLYNSLIR
                     ALQHFGLADAFGVSAIPIFVLNVTFPLVPEEISRFCVGKRAVMIVEEGTPEYIEQEIL
                     ASLRRLEVGTPVHGKDMLTAAGEYTVEVIAQGLEKFFDAYLPGLASEGGKDWLRSNTT
                     RRRDIARQLSGPLPARPPQFCVGCPERPVFAALKLSQRDVGPTHIAGDIGCHSFATFE
                     PFSFGHSILGYGMSLASRAGVSPMMKRRVLSVMGDGGFWHNGLLTGVQSALFNGDDAV
                     LVIFKNGYTSATGTQELISTPDAEDKIAAEDKQQSLVHRNLTIEKTLTGMGVKWMRTV
                     HTYHVEKMRQTFDEAFTTDFNGLKIIIAEGECQLERQRRIRPWIAGLLKAGKRVVRVR
                     YGVDEDVCTGDHACIRLSGCPTLTLKDNPDPLKVDPVATVIDGCVGCGLCGENAHAAT
                     LCPSFYRAEVIRNPRLHERVVAGLRSAVLRWMQPA"
     gene            2267..3841
                     /locus_tag="VN496_08205"
     CDS             2267..3841
                     /locus_tag="VN496_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HXF16972.1"
                     /translation="MADRRPITLLICALGGEGGGVLAEWLVDTATRSGYSAQSTSIPG
                     VAQRTGSTTYYFEVFPVPIAQLGGRVPLFSLYPVPGALDVLVSSELLETVRHIASGMA
                     SRERTLVVSSSSRTLTTAEKLQLADGRASSEELLKIVQQYSKRAHVFDMSSVAQDAGT
                     VLSAVLFGAIAGSGALPFPREAFDYTIRQSNTGVEASLRGFARAFEIVERGRNTKLPA
                     ASMSVSAGADLPSDLAHDFPLQTHALLSAGYARVHEYQDEAYAGLYVQRLKRILEAER
                     LADPSGDRQFATTSEAARYLALWMAFDDIVRVAELKCRASRFARVRAEVKAADGDLVR
                     IYDYFKPGMPEFAALLPARLARALTAWDSQRQQAGKAPVALPLKVGAHTVFGYLSLRL
                     LAGLKWLRKRGSRFAAEQKMMERWLDAVERGARLHWGLGNEIALCGRLIKGYGSTNER
                     GKDNLLHVLEQLAPADKANGPEARAQAIRAARVAALADDAGRELDKTLLEHGAAPRPV
                     KAQPIMWVKKRRGTPA"
     gene            3958..4386
                     /locus_tag="VN496_08210"
     CDS             3958..4386
                     /locus_tag="VN496_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXF16973.1"
                     /translation="MKTVQQLLESKRHSVISVAPSSSVLDALKVMAEKEIGAVVVIDG
                     GHLVGIFSERDYARKVILQGKASKDTLVREIMTEKVVCVRPEQTIEDCMGLMTDKRIR
                     HLPVLEHKRVIGVISIGDVVKEMLYEKEFVIKQLESYIHN"
     gene            4446..4676
                     /locus_tag="VN496_08215"
     CDS             4446..4676
                     /locus_tag="VN496_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16974.1"
                     /translation="MIAITLALSGCKTQGALTADSTHCRTMDVDVIKSRYSREGSTTV
                     WCAKCKDKLYLCVSNPPRSRVECKEAAPEACS"
     gene            complement(4738..5304)
                     /locus_tag="VN496_08220"
     CDS             complement(4738..5304)
                     /locus_tag="VN496_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXF16975.1"
                     /translation="MKTQFAALCLIGTFALPAAAADSYSVDPAHTYPNFEINHLGFST
                     MHGRFGATHGKVMLDQAGKNGSIDITIDASSIDTGHAKRDAHLKSEEFFNVAKFTTLT
                     YKATKLRFNGDKLSGADGELTMLGVTKPASLEVTSFNCGPHPMNKKPMCGANATTTIK
                     RSEFGLSTYVPAVGDEVKITIEIEALKD"
     gene            complement(5379..5933)
                     /locus_tag="VN496_08225"
     CDS             complement(5379..5933)
                     /locus_tag="VN496_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXF16976.1"
                     /translation="MNFRAIVFSFAVLATGVPAHAAEVDYASSQITFVSKQMNVPVQG
                     SFKKFTARIDFDPKKLAAAKAQIDVDLASIDTGSPDADAEVSKKGWFNTSAFPTAKFV
                     SSAVSQSGPDKYEVRGKLSIKGIGQDVVAPFTLKRAGDAVTYEGRFNLKRLQFKIGEG
                     VWSDTDTVADDVQVNFRIVTTGNK"
     gene            complement(5930..6490)
                     /locus_tag="VN496_08230"
     CDS             complement(5930..6490)
                     /locus_tag="VN496_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HXF16977.1"
                     /translation="MQITSPGYTRVAIILHWLIAAALFSTFLLGQYMTQLQLSPVKLK
                     LYSYHKWIGVTIFLLVLLRLVWRLAHRPPAPPAAMPAWQHRAAASAHLLLYILTLAIP
                     ISGWLMSSASGFQVVYLGQIPIPDLVAKNKHLAEQLKEVHEALNWVMLGVVILHIAAA
                     LKHHMVDRDDVLQRMLPFLKKRSALR"
     gene            complement(6492..6944)
                     /locus_tag="VN496_08235"
     CDS             complement(6492..6944)
                     /locus_tag="VN496_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXF16978.1"
                     /translation="MPKEQFFRTLRSLAECYQAFESYSGTHVRKLGLTSSQFDIVATL
                     GNTPGMTCKELGANTLITKGTLTGVLDRLESRGLIRRSATDADRRQVFVTLTAQGVRT
                     FERAFPAHADHLKSAFCALSSRELEQTARLLHKLRDALRNARAAPKAA"
     gene            7096..7836
                     /locus_tag="VN496_08240"
     CDS             7096..7836
                     /locus_tag="VN496_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXF16979.1"
                     /translation="MFGALIIGDEILSGKRQDKHFPKIVALLKARGLGLSWSMNLPDD
                     AEILTQVLTSTLARADIVFSFGGIGATPDDHTRQCAAKAAGVELQLHPDAKREIEARF
                     GVEAYPKRIQMGVFPAGSRIIPNPYNRVPGFSYAKHHFLPGFPEMAWPMMEWVLDTEY
                     PHLHSAQPPVDMAIIVKDAGESQLIDLMNDCIARYAGLKLFSLPSFTPAGRRIELGVK
                     GEAEMAAEALEFLKQGVVALGFAWDPTK"
     gene            7910..8674
                     /locus_tag="VN496_08245"
     CDS             7910..8674
                     /locus_tag="VN496_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16980.1"
                     /translation="MDEGHTVKYMAQVLFLSLLLGGPAMAAGHLADVEIKDRTSGRTL
                     PVYESAGHWFVAGRPGNEYRINIRNRETEDLLAVVSVDGINVVTGEIAGAAQSGYVID
                     RAISLSIAGWRKSLQKIASFYFTDLSDSYAARTGRPDNVGVIGVALFRRHPQPLAQMD
                     PPEASVGAGASNDNAARSASAPAREKRIGTGHGRSQSAPARYVEFERASNSPAEVIAI
                     HYDTYANLVARGVIRDEHRLPLPFPGEFVPDPPRNG"
     gene            8742..9317
                     /locus_tag="VN496_08250"
     CDS             8742..9317
                     /locus_tag="VN496_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXF16981.1"
                     /translation="MSAENVSDEQLMSRYRDGDAVAFEALYGRHRGPVFRYVLRQVGL
                     RSAAEEVFQEIWMNIIAARERYRVQARFATFLYHVAHNCVVDHFRRKTPLHLISLDDD
                     PEEAIQIAGPERDQPERAVASRQSSAKLLNALSLLPPEQREAFLLHEEGGLTLEEIAT
                     VTGTGRETVKSRLRYALAKLREGVEHEADVA"
     gene            9298..10065
                     /locus_tag="VN496_08255"
     CDS             9298..10065
                     /locus_tag="VN496_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF16982.1"
                     /translation="MKPTSPEDQELEKSSAEVSEHYRAAAQDEPPSRVDVAVLEAARR
                     EVKQPARRRRDWQIPASVAAMVIIGVAMALLVRENQAPPSLDVVNEAKLARQTSPQMA
                     MKTPPKANTDSVRERPSRERSPRPDRTLSSQEQMAPAARDSAAPDNVANRAAAPASAP
                     APAKPAESASIAAAESKKSESMSDAAAQSRRSDQALLKQKADNSIAQPEDWLRRIDDL
                     LREGKKADAREQVQVFQKAYPEFVLPQRFKALLVEQH"
     gene            complement(10104..10766)
                     /locus_tag="VN496_08260"
     CDS             complement(10104..10766)
                     /locus_tag="VN496_08260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF16983.1"
                     /translation="MANRTLSLSDALHAYLVEHSVREPDILRRLRAETAQDSMSMMQI
                     SPEQGQFMQLLVKLMGARQCLEIGVFTGYSSLSIALALPMDGHIVACDVSEKWTAIAR
                     KYWKEAGVARKIKLHIAPALETLDNLIAGGQGGVFDFVFIDADKINYLNYYERALVLA
                     RTGGLIAVDNTLWGGQVVDTSSQSEDTRAIRSLNEKLRSDPRVQISLLPIGDGLTLAL
                     KL"
     gene            complement(10835..11725)
                     /gene="gluQRS"
                     /locus_tag="VN496_08265"
     CDS             complement(10835..11725)
                     /gene="gluQRS"
                     /locus_tag="VN496_08265"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HXF16984.1"
                     /translation="MNIYRGRFAPTPSGPLHFGSMVAALGSYLDAKSRGGTWAVRIDD
                     LDPPRVAPGAADAILRCLEKSGMHWDGEVIYQSKRTRAYEAALQQLRANGLVYACSCS
                     RKEIGETLSGGAVYPGTCRHGVAPGRELRAWRVRTEPAEIAFQDLLQGRISQNLEYEI
                     GDFVLYRTDHVFAYHLACAVDDDAQGITHVVRGADLMSSTPRQIYLQRLLGLPTPEYL
                     HLPIALNAAGEKLSKQTLAPALNIARIATVLADALEFLRHPPPHQVRAEGASALLRWG
                     VENWRREELPLIAGSRPSGT"
     gene            complement(11732..12076)
                     /locus_tag="VN496_08270"
     CDS             complement(11732..12076)
                     /locus_tag="VN496_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HXF16985.1"
                     /translation="MIGVARIAASSSLIALILLCIAWELWLAPVRAGGSSLVLKALPL
                     LLPLFGVLRGKRYTYQWSSMLIFAYFMEGVVRGWSEHGAGQALALAEIALSVVFFIAA
                     ISYARLARTLPA"
     gene            complement(12073..12765)
                     /locus_tag="VN496_08275"
     CDS             complement(12073..12765)
                     /locus_tag="VN496_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HXF16986.1"
                     /translation="MSRGSDARRLVRKFTAGVLSTQSLKFRGYPYSSSLPFCTDQRGR
                     VVVLISHLAEHTQNAEHDVKTGFLVSPLTRDLQEQARVSMIGDIAPVDDPVIALRYTR
                     FFPEARQYLQIGGFRFFRIEPRSLRYIAGFGSIHTITADSYLALDYPIADAESEIIEH
                     MNKDHAHTLLDYCRFVYQKTPAGATMIGIDCDGFDVLADGEVLRMEFSSEVTNAGEAR
                     SELVKLAQASRA"
     gene            complement(12779..13372)
                     /gene="wrbA"
                     /locus_tag="VN496_08280"
     CDS             complement(12779..13372)
                     /gene="wrbA"
                     /locus_tag="VN496_08280"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HXF16987.1"
                     /translation="MQEILVLYYSQTGAVKQMAQFVARGIEKVTGMGARLRTVPKVST
                     VIEATEAEVPDSGAPYVDYGDLEQCAGLALGSPTRFGNMAAPVKYFIDSTGGLWLRGA
                     MSGKPAAVFTSTASLHGGQEATLLSMMLPLIHHGMLIVGVPYSEPELMSTRSGGTPYG
                     ASHLAGPNADQPVTEDERRLCIALGTRLAEVAQKLSR"
     gene            13430..>13942
                     /locus_tag="VN496_08285"
     CDS             13430..>13942
                     /locus_tag="VN496_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HXF16988.1"
                     /translation="MKPYLNSIKELGRQFRLLGNFVVLVYRRFKEERCFQLCGSLTFT
                     TMLALVPLITIMATVMTAFPVFDNVVDTLKRYVAGNLVPEGSSKLVTMYMQQFADNAA
                     RLTALGIVLLGVSAIMLMLTIDRAFNTIWRVKRPRSLIQRVLIYWSVLTIGPMLIGGS
                     LSLTSWLVSQS"
BASE COUNT         2912 a         3960 c         4166 g         2904 t
ORIGIN      
        1 aacaacttaa aacgccttat ccggtgtcgt tcaagcgtgt cgagaaatac tggagcgagg
       61 tttagtccgg gctgctagga tttgacgcca gagctaccac acgctacgcc atggaaaaat
      121 cattcaaaaa ggaaatcggg ctgctgagcc ttggtgcggg ccaaattttt tacggcgaag
      181 gcattcttgc gatcaccaag gcgttgctgc agtccggcgt ggcttatgtc ggtggttatc
      241 agggtgcgcc agtttcgcac ctgatcgacg tcatggtcca gggtcgggag tacctgaata
      301 cgctcggcgt gcatgtcgaa gcatgctcga atgaggcgtc cgcggcagcc atgctgggcg
      361 catcgatcca ttacccgttg cgcggcgccg taacctggaa gtcgatcgtg ggtaccaatg
      421 tcgcggccga tgccttgtcg aacttgtctt cacctggcgt cacgggtggc gcgctgattg
      481 tggtcggcga agattatggc gaaggcgcca gcgtaatcca ggagcgcacg cacgcgtatg
      541 cgctgaagtc ttcgatgctg atgctggatc cgcgccccga attgtcgaac atggtccgga
      601 tggtggaaca ggggttccag ctttcggagg cgtcgaacat gcccgcatta atggaactgc
      661 gcatccgcgc ctgtcacgtg cgcggcagtt tcgtatgcaa agacaacatc gccccggcca
      721 tctcgacacg ccatctcatg gcggagccgg cgaaatttga ctatgcgcgg ctggcacacc
      781 cgccggtgac gtttcgtcac gagaagctta aggtcgatga ccgcatccca gccgcgcggc
      841 ggtacatcgc agaaaaccag ctgaatgaac tcatggccgg cgcacgcgct gaagccggtt
      901 ttatcgtgca gggcgggctt tataactcgc tcatccgtgc gttgcagcac ttcggactgg
      961 ccgatgcctt tggcgtgtcc gcgattccca tcttcgtgct gaatgtcacg tttccgctcg
     1021 taccagagga aatttcccga ttctgcgtgg gaaagcgcgc agtgatgatc gtggaagaag
     1081 gcacgcccga atatatcgaa caggagatac tcgcatcgtt gcgcaggcta gaagtcggaa
     1141 caccagtgca cggcaaggac atgctgacgg ctgcgggtga atacacggtt gaggtcattg
     1201 cccaaggtct cgaaaaattc ttcgatgctt atctgccggg cctcgcgagc gaggggggta
     1261 aagactggct ccgaagcaac acaacacgcc ggcgcgacat tgcgcgccag ctttccgggc
     1321 ccttaccggc acgtccgccg caattctgcg tcggctgtcc cgagcggccg gttttcgcgg
     1381 cgctcaaact gtcccagcgg gatgtgggtc cgacgcacat tgcgggcgac atcggttgcc
     1441 attccttcgc gacattcgaa ccgttttcgt tcggccattc gatcctgggc tacggcatga
     1501 gcctggccag tcgtgctggt gtctcaccca tgatgaagcg gcgcgtgctc tcggtcatgg
     1561 gtgacggcgg attctggcac aacggtctgc tgaccggagt gcagtcggcc ttgttcaatg
     1621 gcgacgatgc ggtactggtg atcttcaaga acgggtacac gtccgctacc ggcacccagg
     1681 aattgatctc cactccggac gcagaggata agatcgctgc agaggacaaa caacaaagtc
     1741 tcgtgcatcg caacctcacc atagaaaaga cgttgaccgg catgggcgtg aaatggatgc
     1801 gcaccgttca tacctatcat gtcgaaaaaa tgcgccagac cttcgatgaa gcgtttacga
     1861 ccgacttcaa cggactcaag atcatcatcg ccgagggcga atgccagctt gaacgccagc
     1921 gacgtatccg gccatggata gcgggactgc tgaaggcggg caagcgcgtg gtgcgtgtga
     1981 ggtacggcgt agacgaggac gtttgtaccg gtgaccacgc gtgcatccgc ctgtcgggtt
     2041 gcccgaccct gactctgaaa gataatccgg atccgctcaa ggtggatccg gtggcgacag
     2101 tcatcgacgg ttgcgtcggc tgcgggctgt gcggcgaaaa cgcgcatgcc gccaccttgt
     2161 gtccctcgtt ctatcgcgct gaagtcatcc gcaacccgcg tttgcatgaa cgcgtggtag
     2221 cgggtttgcg cagcgcggtg ctgcggtgga tgcagccggc atgaatctgg cagatcgtcg
     2281 tccgatcact ttgcttatct gcgcgcttgg cggcgaaggt ggcggggtgc tggccgagtg
     2341 gctggtcgat accgctacgc gcagcggcta tagcgcgcag agtacgtcga tacccggcgt
     2401 tgcacagcgc accggttcga caacgtacta ctttgaagtt ttcccggttc cgatcgcgca
     2461 actcggcggc cgcgtgccgt tgttcagcct gtatccggta cctggcgcgc tcgatgtact
     2521 ggtttcctct gaactgctgg aaacagtgcg ccatatcgcg agcggcatgg cttcgcgaga
     2581 gcgcacgctg gttgtgtcct cgagttcgcg gacgctgacc actgcggaaa aattgcagct
     2641 ggccgatggg cgcgcttcat cggaggagct gcttaaaatc gtgcagcaat acagcaaaag
     2701 ggcgcatgta tttgacatgt cctcggtggc ccaagatgcg gggacggtgt tgagcgcagt
     2761 gctgttcggt gcgatcgcgg gcagtggtgc gctgcctttt ccgcgtgaag cgttcgatta
     2821 caccatccgg cagtccaata ccggcgtaga ggccagcttg cgcggttttg cgcgtgcatt
     2881 cgaaatcgtc gaacggggtc ggaacacaaa gctgccggca gcttcgatga gcgtatccgc
     2941 tggcgcggat ttgccatcgg atctcgcaca cgactttccc ttgcagaccc atgcgctgct
     3001 gagcgccggt tatgcgcgcg tacatgaata tcaggacgag gcctatgccg gactttatgt
     3061 tcaacgcctg aagcgcatcc tggaggccga acggttggca gacccgtccg gcgatcgcca
     3121 atttgccacc accagcgagg ccgcgcgtta tctggcgcta tggatggcgt tcgatgacat
     3181 cgtccgtgtc gccgaactga aatgccgggc gagccgcttt gcgcgggttc gcgccgaagt
     3241 caaggcggca gacggcgatc tggtgcgcat ctacgactac ttcaagcctg gaatgcccga
     3301 attcgcggcg ctcttgcctg cgcgtttggc acgcgcactg acggcttggg acagtcagcg
     3361 acaacaggcg ggtaaagcgc ccgttgcatt gccgctgaag gtcggcgcgc ataccgtttt
     3421 cggttatctc tcgttacgtc tgctggccgg tctgaaatgg ctgcgcaagc gtggcagccg
     3481 gtttgcggcg gaacagaaga tgatggaacg ctggcttgat gcagtcgaac gcggcgcgcg
     3541 cttgcattgg ggactgggta acgagatcgc gttatgcggc cgccttatca aggggtacgg
     3601 tagcaccaat gagcggggca aggataactt gttgcacgtg ctagagcagc ttgcaccggc
     3661 cgataaggca aacggcccgg aagcgcgcgc gcaggcgatc cgtgccgcgc gggtggcggc
     3721 actggcggac gacgcgggcc gggaactaga taagacgctg ctcgagcacg gtgcggcgcc
     3781 caggcccgtg aaggcgcagc caatcatgtg ggttaaaaag cggcgcggca ctccggcctg
     3841 aggtgtgccc agagaaaatc gtgtgcgggg ttgcgcaccg ttacgttgac tactatgctt
     3901 aagagcgtag tctgaagcca accttcaccg tcaggactga ctagcgaggg gaacaaaatg
     3961 aaaacagttc agcagctgct ggaatccaag cgccatagcg tgatctcggt tgcgccatcg
     4021 agttcggtac tagatgcgct gaaagtaatg gcggaaaagg aaatcggcgc tgtggtcgtg
     4081 atcgacggcg ggcatctggt cggcatattt tccgaacgcg attacgcgcg caaagtgatc
     4141 ctgcagggga aggcttcgaa agatacgctc gtaagggaaa tcatgaccga aaaggtcgtg
     4201 tgcgtgcgtc cggaacagac tatcgaagat tgcatggggc tgatgaccga caagcgcatt
     4261 cgtcacttgc ccgtactcga acacaagaga gtaatcggcg tcatttcgat tggtgatgtc
     4321 gtgaaggaaa tgctttacga aaaggaattc gtcatcaaac agctggaaag ctacattcac
     4381 aattagaggg cccgcctggg gcggccggca tccagtttcc attggcgaat accttcattt
     4441 gcgtcttgat cgcaatcacg ctcgccctgt cgggctgcaa gacccagggc gcgttgactg
     4501 ccgattcgac ccactgccgc acgatggacg tggacgtgat caagagccgc tattcgcgcg
     4561 aaggttccac caccgtgtgg tgcgcaaaat gcaaggacaa gctttacctg tgcgtatcga
     4621 atccaccgcg cagccgggtg gaatgtaagg aagccgcgcc ggaggcctgc tcgtaagcag
     4681 acctgaagca aaaaaaaggg cagcgtcaag ctgccctttg ttgtttactg cctgcgatca
     4741 atccttgagt gcctcgattt caatggtgat ctttacttca tcgcccactg ccggtacata
     4801 agtgctgaga ccgaactcgc tgcgcttgat ggtcgtcgtg gcgtttgcac cgcacatggg
     4861 cttcttgttc atcggatgag gtccgcaatt gaaggaagtg acctccaggc tcgctggttt
     4921 cgttacgccc agcatggtga gttcgccgtc tgccccgctc aatttgtccc cgttgaacct
     4981 gagtttggtg gctttgtacg tcagcgtcgt aaacttggct acattaaaaa attcctcgct
     5041 tttgagatgc gcatcgcgct tggcatggcc ggtatcaatt gaactcgcat caatggtgat
     5101 gtcgatgctg ccgttcttgc ccgcctggtc gagcatgacc ttgccgtgtg ttgcgccaaa
     5161 gcggccatgc atggttgaaa atccaaggtg gttgatttcg aaattcggat aagtatgagc
     5221 cgggtccacg gagtacgagt cggctgcggc cgcaggcagc gcaaatgtgc cgatcaagca
     5281 gagtgctgca aattgcgttt tcaaattgaa tctcccatgt tgattgaaca aggtgctgcg
     5341 ataaaaagtg cccgacgcca ctcgcgattg tgcggacgct atttgttgcc tgtggtcacg
     5401 atacgaaaat tcacttgtac gtcgtctgcc acggtatcgg tgtccgacca cacgccctca
     5461 ccgattttga actgcaggcg tttcaggttg aagcggcctt catacgtgac tgcatctcca
     5521 gcgcgcttca aggtaaaggg cgccacgaca tcctggccga tgcctttgat ggaaagtttt
     5581 ccgcgtacct cgtatttgtc cggcccggac tgcgataccg cactggacac gaatttcgcc
     5641 gtcgggaacg cactggtgtt gaaccagcct ttcttgctca cttccgcatc ggcgtcgggg
     5701 ctgccggtat cgatactggc tagatcgacg tcgatctgcg ccttggcggc agcgagtttt
     5761 ttgggatcga agtctatgcg tgccgtgaat ttcttgaagc tgccctgcac cggcacgttc
     5821 atctgcttgg acacgaaggt gatctggctg ctggcgtagt cgacctcagc ggcgtgcgca
     5881 gggacaccgg tcgcgagcac tgcaaacgaa aagacaatgg cgcgaaagtt catctgagtg
     5941 cgctccgttt cttgaggaat ggcagcatgc gctgcaatac gtcgtcgcga tcgaccatat
     6001 ggtgtttaag ggcggccgcg atgtgcaata tgacgacgcc gagcatgacc caattcaatg
     6061 cctcgtgcac ttccttgagt tgttccgcaa ggtgtttgtt cttggccacc agatccggaa
     6121 tcggaatctg tccaagataa acgacctgga agcctgaagc agagctcatc agccatcctg
     6181 agatgggaat ggccagtgta aggatataca gaagcaaatg agctgaagca gcagcccgat
     6241 gctgccatgc cggcatcgcg gccggcgggg ccggtggccg gtgggccagt cgccagacga
     6301 gccgcaacaa taccagcaga aaaatcgtga ccccaatcca cttgtgatac gagtacagct
     6361 tcagcttgac aggagaaagc tgcagttgcg tcatgtactg accgagcagg aaggtgctga
     6421 agagggccgc cgcgatcagc cagtgcaaga tgattgcgac ccgggtatag cctggcgaag
     6481 tgatttgcat gtcaggcggc ctttggtgcg gcgcgcgcat tccgcaatgc atcgcgcagc
     6541 ttatgcaaaa gtctcgccgt ctgctccagc tcccgcgaag ataacgcgca gaacgccgac
     6601 ttcaggtgat cagcgtgtgc cgggaatgcc cgctcgaatg tgcgcacgcc ctgcgccgta
     6661 agcgttacga atacctgcct gcgatctgca tcggtcgcgc tacgccggat caggccgcgg
     6721 ctttccagcc ggtccaggac gccggtcagc gtgcctttgg taatgagcgt attagcaccc
     6781 agttccttgc atgtcatgcc cggggtattg ccgagcgtgg caacgatatc gaattgagag
     6841 cttgtcaggc caagcttgcg gacgtgtgtg ccggaatatg attcgaatgc ctgatagcac
     6901 tcggccaatg aacgcaaggt tctaaagaac tgctccttag gcatatcgcc gccgtccgtg
     6961 catttagttc taatgcgaac tattcttgcg gttggcgcgg gcgaagtcaa cgcgtttgct
     7021 ccagtcgccc gcttgacggg tgttccccac tgcccgcaaa ctcgccgcac tcaatagttc
     7081 gacaggagca gcacgttgtt tggagcgctc atcattggcg atgaaattct ttcgggcaaa
     7141 cgccaggaca agcatttccc gaagattgtc gcactgctga aggcgcgcgg actcgggctg
     7201 tcatggagca tgaacttgcc cgacgatgcc gaaatcctca cgcaagtgct gaccagcacg
     7261 ctggcgcgcg cggatatcgt gtttagtttt ggaggcattg gcgccacgcc ggacgatcac
     7321 actcgccaat gtgccgcgaa agcggcaggc gtcgaactgc aactgcatcc cgatgccaaa
     7381 cgtgagattg aagcgcggtt cggcgtagaa gcgtatccca agcgcataca gatgggagtg
     7441 tttcccgcgg gcagccggat tattccgaat ccctacaacc gtgtgccggg attcagctat
     7501 gcaaagcacc attttttgcc tggctttccg gaaatggcct ggccgatgat ggaatgggtg
     7561 ctcgataccg agtatcccca cctgcatagc gcgcagccgc ccgtggatat ggcgatcatc
     7621 gtcaaagacg caggcgagag ccagctgatc gatctgatga acgactgcat tgccaggtat
     7681 gctgggctga agctgttcag cctgccaagc tttacgccag cgggccgccg catcgaactc
     7741 ggcgtcaaag gcgaggcaga gatggccgct gaggcgctgg agttcctcaa gcagggtgtt
     7801 gttgcgctcg gatttgcatg ggatccaaca aaataacgtc gcaccagccg ttaattttgc
     7861 ggtcgtctaa ccccgtttca ttctgacctg cgtttacggc ggtgttctca tggacgaggg
     7921 gcataccgtg aaatacatgg cgcaggtttt gttcctgtcg ttgttgcttg gcggtccggc
     7981 gatggcggct ggccacctgg ccgatgttga aatcaaggat cgcactagcg gacgtacgtt
     8041 gccagtctac gaaagcgcgg ggcattggtt tgtggccggt cggcccggaa atgaataccg
     8101 gatcaacatc cgcaaccgcg aaactgaaga cttgcttgcc gtcgtttctg ttgatggcat
     8161 caatgttgtg acaggtgaaa tagccggagc cgcgcaaagc ggttacgtga tcgaccgtgc
     8221 aatttcctta tccatcgccg gttggcgcaa gagcctgcaa aaaatcgcgt ctttttattt
     8281 caccgatctg agcgattcct atgccgcccg taccgggcgt ccggacaatg tcggcgtgat
     8341 tggtgttgcg ctgttccggc ggcatccaca accgcttgca caaatggacc ctccagaagc
     8401 gagtgttggt gccggagctt cgaacgacaa tgcggctcgc tcggccagcg cgccggcaag
     8461 agagaaacga atcggcaccg gccacggccg cagccagtct gctccggcac gttatgttga
     8521 attcgagcgc gcatcgaata gcccggcaga agtgatcgcc atccactatg acacctacgc
     8581 caatctggtc gcgcgcggcg taatccgcga cgagcacaga ttacctctgc cttttcccgg
     8641 cgaattcgtt cctgatccgc cgcgaaacgg ctaatcccat gccagggaac tgaacctcat
     8701 tcgggatgtc atgaccgatg cttccggcta gactggcgca cgtgagcgcc gaaaacgtct
     8761 ccgatgagca actgatgtcg cgctaccgcg acggcgatgc ggtggcgttc gaggcgttgt
     8821 atgggcgcca tcgtggcccg gtgtttcgtt atgtattgcg ccaggtcgga ttgcgctcgg
     8881 cagccgaaga ggtattccag gaaatctgga tgaatatcat cgccgcgcgg gagcgctacc
     8941 gggtgcaggc gcgttttgcg acttttctgt atcacgttgc gcacaattgc gtggtcgatc
     9001 actttcgtcg caagacgccg ctgcatttga tttcgctcga tgacgatccc gaagaggcga
     9061 tccagattgc gggtcccgag cgtgatcagc cggagcgcgc agtagcatcg cggcaatcgt
     9121 ccgcgaaact gctcaacgcg ctgtcgctgt tgcctccgga acagcgagaa gcttttttgt
     9181 tgcatgagga aggtggtctg acgctggagg aaatcgccac cgtcaccggt accggcaggg
     9241 aaacagttaa aagtcgcctg cgctatgcgc tggcgaaact gcgcgaagga gtcgagcatg
     9301 aagccgacgt cgcctgaaga ccaggaactg gagaaaagca gtgcggaggt ttcggaacac
     9361 taccgtgctg ctgcccagga cgagccgcca tcgcgcgtcg atgtcgcggt gctcgaggcc
     9421 gcccgtcgcg aggtcaagca gccggcacgc cgccggcgtg attggcagat acccgcatcg
     9481 gttgccgcaa tggtgatcat cggcgtcgct atggcgctcc tggtgcgaga aaaccaggcg
     9541 ccgccatcgc ttgatgtcgt caatgaagcg aagctcgcca ggcaaacatc gccgcaaatg
     9601 gcgatgaaga caccgccaaa ggcaaacacc gactccgttc gcgaaaggcc ttcccgggaa
     9661 cgcagcccgc gtccggatcg cacgctatcc tcgcaggaac aaatggcccc ggctgcccgg
     9721 gacagcgcgg cgccggacaa cgtggccaat cgggcagcag ctccggcctc ggcacccgcg
     9781 cctgcaaagc cagcagaatc agcaagcatt gccgcagccg agagcaagaa gtcggagtca
     9841 atgtctgatg cggccgcgca atctcggcgc agcgatcagg cgctgctcaa gcaaaaagcc
     9901 gacaattcca ttgcccagcc tgaggactgg ctgcgcagga tcgatgattt gctgcgcgaa
     9961 ggcaaaaaag ccgacgcacg cgagcaggtg caggtgtttc aaaaggctta tccggagttt
    10021 gtgttgccgc agcggttcaa agcgttgctg gtcgagcaac attgaactgc cgcatggtgg
    10081 caatctcgca cacgaaccgt gccctacagc ttcagcgcaa gcgttagtcc atcgccgatc
    10141 ggcaatagtg aaatctgcac gcgcggatcg gaccgcagtt tttcattcag gctccgtatg
    10201 gcccgtgtat cttcgctttg ggacgacgta tccacgacct gaccgcccca aagcgtgttg
    10261 tccactgcga ttagtccgcc cgtgcgggct aataccaggg cccgctcgta ataattcaaa
    10321 taatttattt tgtcggcgtc aataaacacg aaatcgaaga caccgccctg accgcctgca
    10381 atgagattgt cgagcgtctc cagcgccggt gcgatgtgca gtttgatttt ccgggcgaca
    10441 cctgcttcct tccagtactt gcgcgcgatt gcggtccatt tctcggagac atcgcaggcc
    10501 acgatgtgac cgtccattgg cagcgcaagc gcgatggaca gactgctgta accggtgaac
    10561 acgccgattt cgaggcattg tctggcgccc atgagtttga ccagcagctg catgaactgt
    10621 ccttgttccg gactgatctg catcatcgac atgctatctt gcgccgtttc tgctcgcagg
    10681 cggcgcagaa tatcgggctc acgcactgaa tgctcgacca gataggcatg cagcgcgtcg
    10741 gaaagcgaaa gtgttctatt ggccattgct cttatgataa ccgtgccgca attacatgat
    10801 gcgaaatgca tcagtacggc atgccgcaca ggcgttaagt tcccgaaggg cgcgaacccg
    10861 caatcaatgg aagctcctca cgcctccagt tctcgacgcc ccaacgcaac agcgcggatg
    10921 cgccttcggc acgcacttga tgcggcggcg gatgccgcaa aaattcaagt gcatctgcca
    10981 gtaccgtagc tatccgggca atattgagcg ccggtgcgag cgtctgcttg gaaagttttt
    11041 cacccgctgc attcagggcg atcggcagat gcaggtactc gggtgttggc agacccagca
    11101 gtctctgcag ataaatctgg cgcggggttg aagacatcag atccgcgcca cgcacaacat
    11161 gcgtaatgcc ttgtgcgtca tcgtccacgg cgcaggcgag gtgataggcg aagacgtgat
    11221 cggtgcgata aagcacaaag tcgccgatct cgtactcgag gttctgactg atccggcctt
    11281 gcaacaagtc ctgaaacgct atttccgcag gctcggtgcg gacgcgccag gctcggagtt
    11341 cgcgtcccgg cgcaacgcca tggcggcagg ttcccggata gacggcgccg ccggaaagcg
    11401 tttcaccgat ctcttttcga ctgcaactac acgcgtagac caggccattg gcgcggagtt
    11461 gctgcagcgc tgcctcgtaa gcccttgtgc gtttgctttg ataaataacc tcgccatccc
    11521 agtgcatccc cgatttttcc aggcagcgaa gaatcgcgtc ggccgcacca ggggcgaccc
    11581 ggggcgggtc gagatcgtca atgcgcacag cccaggtgcc gccgcgcgat tttgcatcca
    11641 ggtagctacc cagggctgcg accatcgatc cgaagtgcag gggtccggaa ggggtcgggg
    11701 caaatcttcc gcgataaatg ttcaagaacg gctatgccgg taacgtacgg gccaggcgtg
    11761 cgtagctgat cgccgcgata aagaaaacaa ctgatagtgc aatttccgca agcgccaatg
    11821 cctgtccggc tccgtgctcg ctccacccac gcaccactcc ctccatgaag taagcaaata
    11881 tcagcatcga ggaccactga taggtgtaac gtttgccgcg caggacgccg aacaggggca
    11941 gcaatagtgg aagcgcttta aggacgagcg aggaaccacc agcacgcacc ggcgccagcc
    12001 acaattccca tgcaatacac agcaggatca gtgcgatgag gctcgaactc gcggcaatac
    12061 gcgcaacacc gatcacgcgc ggctggcctg cgcaagtttt acgagttcgc tacgcgcctc
    12121 gcctgcattg gtgacttcgc tcgagaattc catgcgcagc acttcgccgt ccgccagcac
    12181 atcgaagcca tcgcaatcga tgccgatcat cgtcgcccct gcaggcgttt tttggtacac
    12241 gaagcggcaa tagtccaaca gagtatgcgc atggtccttg ttcatatgct cgatgatttc
    12301 ggattccgca tcggcaatcg gataatccaa tgccaaataa ctgtcggcgg tgatcgtatg
    12361 gatactgccg aacccggcga tgtaccgcag tgaacgcggc tcgatccgaa aaaagcgaaa
    12421 gccgccaatc tgcaggtatt gcctcgcttc cgggaaaaag cgcgtatagc gtaacgcaat
    12481 tacaggatcg tccacaggcg cgatatcacc aatcatgctc acgcgtgcct gttcctgcag
    12541 atcgcgagtc agtggagaca ccagaaagcc ggttttaaca tcgtgctcgg cattttgcgt
    12601 atgttcggca agatgactga tcaacacgac gactctgcca cgctgatcgg tgcaaaacgg
    12661 caacgaagaa gaatatggat agccgcgaaa tttcaacgac tgtgttgaga gcacgccggc
    12721 cgtaaacttg cgtaccagtc tgcgtgcatc agatccgcgg ctcacgtcgg gttaccgttt
    12781 accgggacag cttctgcgct acctcggcga gccgtgtccc aagcgcaatg caaaggcgcc
    12841 gttcgtcttc ggtgaccggc tggtccgcat tggggccggc gaggtgactt gcgccgtacg
    12901 gcgtgccgcc gctgcgcgtg ctcatcaatt cgggctcgga atacggcaca ccgacaatca
    12961 gcatgccgtg atggatcagc ggcagcatca tggacagcaa agtcgcttcc tgtccgccat
    13021 gcagcgacgc cgtagaagta aaaaccgccg ccggctttcc gctcatggct cccctgagcc
    13081 ataagccgcc ggtgctgtcg ataaagtact tcacgggtgc cgccatgttg ccaaaccggg
    13141 ttgggctgcc gagcgcaagc ccggcgcact gctcgagatc gccgtaatcc acgtagggcg
    13201 cgccgctatc gggcacttcc gcctcggtgg cctcgataac cgtggaaact ttcggaacag
    13261 tgcgcagccg tgcgcccatt cccgtcactt tttcgatgcc gcgtgcgaca aattgcgcca
    13321 tctgcttcac cgcgccggtc tggctgtaat agagcaccaa gatttcctgc attgcctgtt
    13381 ttcccgcctt ggaaggccgt attatatcga cacgattctt cggtaaaaca tgaaaccgta
    13441 tctgaattca attaaagaac ttggacgcca attccgcctg ctcgggaact ttgtcgtact
    13501 ggtttaccgg cgtttcaagg aagagcgctg ctttcagttg tgcggcagtt tgaccttcac
    13561 cacgatgctt gcgctggtgc cgctgatcac gatcatggcc acggtcatga cggcatttcc
    13621 ggtcttcgac aatgtcgtcg acacgctcaa acgttacgtg gcgggcaacc tggtgcccga
    13681 aggatcgtca aagctggtga ccatgtatat gcagcagttt gccgacaacg ctgcgcgtct
    13741 tacggcgctg ggcatcgtgc tgcttggcgt ctcagccatc atgctgatgc tcaccatcga
    13801 ccgggcgttc aacaccatct ggcgcgtgaa acggccgcgt tctttgatcc agcgcgtgtt
    13861 gatctactgg tcggtactga ccatcggccc aatgctgatt ggcggcagtt tgtcgctgac
    13921 ttcctggctg gtgtcccagt cg
//
LOCUS       DATMZQ010000255        10160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4000461, whole genome shotgun sequence.
ACCESSION   DATMZQ010000255 DATMZQ010000000
VERSION     DATMZQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4000461"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VN496_08290"
     CDS             complement(<1..974)
                     /locus_tag="VN496_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HXF16989.1"
                     /translation="MTDNQMQNFFAPCPRGLETVLADELAQLGARALANTDGGISFSG
                     PMTLAYAANLHSRIASRILWRVGSGHYGSEDDIYGGVNGLDWPQWFDGALTIRVNVAA
                     IRSPLESLDFITLRIKDAVCDSFRTRTGMRPTVDTAQPDVRIHAFLETERFTVYLDTS
                     GEPLFKRGARKSGGQAPLRENLAAGILSLCGWTPDLPLLDPMCGSGTFLIEALQIARG
                     IAPGSHRNFAFEKLRNFDEPAWRGIKAAAEAKPAPNPAPAIFGSDLYGDALKTARLNL
                     DAAGLADGVTLKQANVLELSAPAAQGVLVGNPPYGVRLGEQEQLTGFYR"
     gene            complement(1014..1397)
                     /locus_tag="VN496_08295"
     CDS             complement(1014..1397)
                     /locus_tag="VN496_08295"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HXF16990.1"
                     /translation="MHAARVLFVFAALACTVLQAAQPTTRILIQSSPLAGFQFHEGKR
                     LWEQLQVGDALMLVREPDNPHDSRAVRVHWNGHMLGYVPRAENDAVARQLDRGNKLEA
                     RIVRLTKHRDPWKRIEFEVFIRLQP"
     gene            complement(1403..1816)
                     /locus_tag="VN496_08300"
     CDS             complement(1403..1816)
                     /locus_tag="VN496_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HXF16991.1"
                     /translation="MLWIKSFHLVFMVTWFAGLFYLPRLFVYHAMSNDAVSRERFKIM
                     ERKLFYGIMTPGGVLTIGFGLWLWLGYGFHGAWLYAKMALVGVLVCYHLYCGRLMNDF
                     KHDRNRRGHMFYRLFNEFPVIILIAIILLVELQPF"
     gene            complement(1904..2518)
                     /locus_tag="VN496_08305"
     CDS             complement(1904..2518)
                     /locus_tag="VN496_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF16992.1"
                     /translation="MKAPPARRLSIDGPAGSLEIAVNESAEEPRGLALLAHPHPLYGG
                     TLDNKVVQTLAKAFVALGYCALRLNFRGVGASSGVFDDGRGEVDDFLALADYGRRTFG
                     ERKLAIGGFSFGAFIAASVVQGLTPTHLVLAAPAVGRFPLGSVPVNTLVIHGEEDEVV
                     PLKEVFEWARPQALPVIVFPGAGHFFHGNLVGLQRVVERHCRTE"
     gene            complement(2515..2823)
                     /locus_tag="VN496_08310"
     CDS             complement(2515..2823)
                     /locus_tag="VN496_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXF16993.1"
                     /translation="MSYYKHHVFFCCNQRKNGEACCADHDAQKMRDYAKSKIKSLQLA
                     GRGKVRINQAGCLDRCQEGPVIVVYPEEVWYTYVDQEDIDEIIEQHLVNGRIVDRLRI
                     "
     gene            complement(2848..3201)
                     /locus_tag="VN496_08315"
     CDS             complement(2848..3201)
                     /locus_tag="VN496_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HXF16994.1"
                     /translation="MTEATQAAAASLDSLQDLRERLRHFAAERDWNQFHGPKNLAMSV
                     AIEAAEIMEHFQWLTPAQSLELGHAARREVAHEIADVLLYLVRLADVLDIDLLSAARE
                     KIALNAQKYPVPRSL"
     gene            complement(3198..4340)
                     /locus_tag="VN496_08320"
     CDS             complement(3198..4340)
                     /locus_tag="VN496_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXF16995.1"
                     /translation="MSGSLAFPPARYARNSRLVAYLTVAYLLLVGYASLYPFSDWRIP
                     GYEAARFLFAGWPPYIVASDVVVNIVAYFPLGLLLTLFWMGYVPRLRAMVLAVGTGVL
                     LSLAIEFAQACVPTRISSNLDVLTNSIGALWGAAAAYAWGQRWLLSGELRRLRERHFQ
                     GGARTDLAFLLLFLWLLTQFNADILLFGNGDLRHIFKLNSGLRYSAEVYLLLEAGITA
                     INFVGIACLVGTITRSFAATAVAVTVIMLSALAFKTIAAMALFIPGDPGLWLTPGSAL
                     GLVAGVMVWPALAQSKPVNLMRAATLCFVVGFLLVNFAPANPYLIAALRILQRGHLLP
                     FNAMIGLLSSWWPALVLLYCLFSLMTNPAARITRLVDVADPKEHMP"
     gene            4389..5354
                     /locus_tag="VN496_08325"
     CDS             4389..5354
                     /locus_tag="VN496_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXF16996.1"
                     /translation="MGFYDIFNGDADGLCALQQLRLAEPRAATLVTGVKRDIALLGQV
                     SPKAGDELTVLDVSLDSNRDALLRTLAVGAHVRYFDHHFAGEIPTHALLETHIDMSAT
                     TCTSLIVDGYLDGRHRMWAIVAAFGDNLPAQAYAAAAELGIGSDDAETLKDLGENLNY
                     NAYGANLAELHFHPATLYLRMRPYADPLAFCAHAPELDTLRRAREDDLRHARALPLEK
                     VGEHAVLVCLPQAAWSRRVIGTFANELARQHAKRIVALLVRTGAGYQVSLRAPEQGEA
                     AMHLLARQFESGNGRARAAGIAFLPDSEVTRLRALLNKPPSPEPA"
     gene            complement(5390..6445)
                     /locus_tag="VN496_08330"
     CDS             complement(5390..6445)
                     /locus_tag="VN496_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF16997.1"
                     /translation="MKKLLPLLLFIALPALAADELHLYNWNDYIAPESVKEFEAQCKC
                     KVVQDFYGDNEELVAKLAAGAKGYDVMVPTSNYVAGMAKAGWLQPLDKSKLPNFKNLM
                     PAYLNTPFDPGNKYSVPYAMSITLIGYNDQKMKELGITVDSWAVIFDPKILQKIKGKI
                     TVLDSQAEVMAAALKYLGYSVNDRDPAHWNQAKDVILKAKPYWAAFMAQGYIKELSTG
                     NIWVVHGYSNDIFQADAGAQEAKQKFRIRHALPKEGAVLALDAMVIAKTAPRADLAHQ
                     FINFMLEGKNSAALTNITGSGNPNAEAMQYIKPEIKSIKAVFPDAQSAKRLEQLKDMN
                     KKERQLLNKIWTEIKAK"
     gene            complement(6547..7320)
                     /locus_tag="VN496_08335"
     CDS             complement(6547..7320)
                     /locus_tag="VN496_08335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF16998.1"
                     /translation="MNKRVVLLWSAAILAYAFLYAPLAIVVVYSFNDSRLNAEWVGFT
                     LSWYQKLFHNEKMLHAAWNSLLIGITASAVSTILGTMAGYAMYRFRTRLLPVLILAPI
                     AIPEILMGVSLLIFFVLLNISLGMVSIVLSHIAFCIGFVAIVVRSRLAGMDESLTEAA
                     RDLGATPWTAFRLVTLPLIMPGVVAGALMAFTLSIDDFVITFFTAGVGSTTLPLQIYT
                     MVKIAVTPEVNAVSTLLMTLTLALILIASRLAPGSLRTQ"
     gene            complement(7317..8207)
                     /locus_tag="VN496_08340"
     CDS             complement(7317..8207)
                     /locus_tag="VN496_08340"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF16999.1"
                     /translation="MAAQQNRLHKWLISGPPLAYLIVFFAIPVLIMVLASFRNPGEFG
                     GLAPLLNDESGSREIDLTTESYGRFFGDFLYTELFLKSLAYAGVTTLACLLIAYPLAM
                     LIARSSKRHRDLLVLLVILPFWSNFLIRVYAWMIILGPQSVFVSALNHIVGWFGLAPL
                     TLLYTPFAVIIGLVYVHLPFMVLPLYANLEKHDPALLDAAQDLGANAWRRFWRVTFPL
                     SLPGVYAGAALVFIPALGIFAIPDILGGTEGIMIGNVIKQQFLDTRDWPFGSVLSMVL
                     TVLAIVLAGAAAWIAKRART"
     gene            complement(8211..9296)
                     /locus_tag="VN496_08345"
     CDS             complement(8211..9296)
                     /locus_tag="VN496_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17000.1"
                     /translation="MPLLEIRNVTRRFGTFAAVDQVSLNIEAGEFFTLLGPSGCGKTT
                     LLRMIAGFDVPDGGSIVLDGKDMVGIPPEARNVHTVFQSYALFPHMNVEQNVAFPLKM
                     ANVDPARIAQRVEETLEDVRLTGMGKRYPNELSGGQKQRVAVARALINRPKLLLLDEP
                     LAALDAKLKESVQLELIALQKEIGITFVYVTHDQSEALALSHRIAVMNQGRVEQVDEP
                     SKIYGFPKNRFVADFIGQCNLLDGQIKACSGERMTVALSGIGDVVAINMEGDAPGLKG
                     ALALRPEKVAIGRDLPADAEANCFTGKVHDLLYLGDVTIYIVELANGMRIEALLPNSA
                     AGRTKFFEAGDEVQIAWRHDAGHFLND"
     gene            9514..>10160
                     /locus_tag="VN496_08350"
     CDS             9514..>10160
                     /locus_tag="VN496_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17001.1"
                     /translation="MKAAAQSAPGGVRAPDANRWWQLIIGIICMSMIANLQYGWTLFV
                     NPLDDKFQWGRTSIQVAFTIFILAETWLVPLEGYLVDRFGPALITVSAGLLVGLSWVL
                     DSLADSLALLYFAAALGGIGAGAVYGTCIGQALKWFPDRRGLAAGLTAMGFGAGSALT
                     VIPLASMIKSSGYQATFLYFGIAQGLVVFALGWLLKKPGPGPGIAHGIRPAPERH"
BASE COUNT         2255 a         2946 c         2908 g         2051 t
ORIGIN      
        1 cgataaaacc ctgtcagttg ctcttgctcg ccaagccgca caccgtatgg tggattccct
       61 accagcacgc cctgtgcagc gggtgcagag agctcgagaa catttgcctg cttgagagtc
      121 accccatcgg cgaggccggc cgcatccaga ttcaaacgcg ccgtttttag cgcatcacca
      181 taaaggtcgc ttccgaagat cgccggcgcc gggttcggcg ctggcttggc ttcggctgcc
      241 gccttgatgc cgcgccaggc aggttcatca aagttgcgta gtttttcgaa agcaaaatta
      301 cgatgcgacc caggtgcaat tcctcgtgca atctgcagcg cctcgatcag gaaagtaccg
      361 ctgccgcaca ttggatcgag caacggcaga tcgggagtcc agccgcaaag gcttaggatg
      421 ccggcagcga gattttcgcg tagaggcgcc tggccgccgc tcttgcgtgc accgcgcttg
      481 aacagtggtt cccccgaagt atcgagatag acagtaaaac gctccgtttc caaaaaagca
      541 tggatacgca catcgggctg ggcggtatcg acggtcggtc gcatgcctgt gcgcgtacga
      601 aagctgtcgc agacagcatc cttgatgcgc aacgtgatga aatcaagact ctcgagcggg
      661 cttcgtatag cggcgacatt gacccgtatc gtcagcgcgc catcgaacca ttgaggccag
      721 tccagcccat tgaccccgcc atagatatcg tcttcgcttc cgtaatggcc tgatccgacc
      781 cgccataaaa tccggctcgc gatacggctg tgcagattag cggcgtaggc taacgtcatc
      841 ggaccagaaa agctgatccc accatccgta ttggccagtg cgcgcgcgcc cagttgtgca
      901 agttcatctg ccaggactgt ctccagcccg cgcgggcagg gagcaaaaaa gttctgcatc
      961 tgattgtcgg tcataggcgc catccagtac ctgagcgcgg cagtgaaata ttgttatggt
     1021 tgcaggcgaa tgaacacttc gaattcaatg cgcttccagg gatcgcggtg tttcgtgagc
     1081 cgcacgatac gcgcttcaag cttgttgccg cgatcaagct gccgcgccac cgcgtcattt
     1141 tcagcgcgtg ggacatagcc cagcatgtgg ccattccagt ggacacgcac ggcgcgggaa
     1201 tcatggggat tgtccggttc gcgcaccagc atcagcgcat cgcctacctg aagctgttcc
     1261 cacagccgct tgccttcgtg aaactggaat ccagctaacg gcgagctttg aatcaggatg
     1321 cgcgtcgtcg gttgtgccgc ctgcagcact gtgcaagcca acgcagcaaa gacaaacaga
     1381 acccttgccg cgtgcatatg catcagaagg gctgtagttc caccagcagg atgatcgcga
     1441 tcaaaatgat caccgggaat tcgttaaaca ggcggtagaa catgtgaccg cgacgattgc
     1501 gatcatgctt gaagtcattc atcaacctgc cgcagtagag gtgatagcac accagcactc
     1561 caaccagtgc catcttggcg tacagccaag cgccatgaaa gccgtaaccc aaccatagcc
     1621 acagaccaaa cccgatggtc aatacgccac caggggtcat gatgccgtag aagagtttgc
     1681 gttccatgat cttgaaacgc tcgcggctca ccgcgtcgtt gctcatggcg tgatagacga
     1741 acagtcgcgg gaggtagaac aggccggcga accacgtcac catgaagacg aggtgaaacg
     1801 atttgatcca cagcatcgtg ttgtcttttt cagtaaagca gccaggtacc cgagtcaaag
     1861 ccgctgccaa ggacggctgc gctttgtatg tagaacagca gattcattcg gtgcggcaat
     1921 gccgctctac aacgcgttgc agcccgacaa gattgccgtg aaagaaatgg ccagcgcccg
     1981 gaaaaacgat caccggcagc gcttgtggtc ttgcccattc gaatacttct ttcagcggca
     2041 cgacctcgtc ctcctctcca tggattacca gcgtgttgac cgggacactt ccgagcggaa
     2101 agcgcccgac agcaggggcg gccaacacca ggtgtgtcgg cgtcaggcct tgcacgacag
     2161 acgcggcgat gaacgcccca aacgaaaatc cgccgatggc aagcttgcgt tctccaaagg
     2221 tgcgccgtcc ataatcggcg agcgccagaa aatcgtcgac ttcgccgcgt ccatcatcga
     2281 atacaccgct actcgcgccc actccgcgga aattgagccg caaagcacag taacccagcg
     2341 ctacaaacgc tttggccaag gtctggacaa ccttgttgtc cagcgtgcct ccatacagcg
     2401 gatgcggatg ggccagcagc gctaagccgc gcggttcttc ggccgattcg ttgacggcaa
     2461 tctcgaggct tccagcgggc ccatcgatgg aaagcctgcg cgcaggcggg gctttcaaat
     2521 gcgcaggcgg tcgacgatgc ggccgttcac cagatgctgc tcgatgatct cgtcgatgtc
     2581 ctcctgatca acgtaggtgt accagacctc ctcgggataa accacgatca ccggcccttc
     2641 ctggcaacga tcaaggcaac cggcctgatt gatgcgcacc ttgccccgtc ctgcgagctg
     2701 cagtgatttg attttggatt tggcatagtc gcgcattttc tgcgcgtcat ggtctgcgca
     2761 acaggcttcg ccattcttgc gctggttgca gcagaaaaac acatggtgtt tgtagtagct
     2821 caactgaaat cctcgtgaac cgctccatta taaactgcga ggcaccggat acttttgggc
     2881 gttcagcgct attttttcgc gcgctgcgga gagcagatcg atatccagca cgtcggctaa
     2941 tctcacgagg taaagcagca cgtcggcgat ttcatgcgcg acttcccggc gcgccgcgtg
     3001 acccagttca aggctttgcg cgggcgtgag ccattgaaag tgctccatga tctccgctgc
     3061 ttcaatggca accgacatgg caaggttctt cggtccatgg aattgattcc agtcgcgttc
     3121 ggctgcgaaa tggcgcagtc gttcgcgcaa atcctgcagg ctgtccaagc tggcagccgc
     3181 ggcctgggtg gcttctgtca tggcatatgc tcctttggat ccgcaacgtc gactagcctg
     3241 gtaatgcgag cggccggatt cgtcatgagc gaaaacaggc agtacagcaa caccagtgcc
     3301 ggccaccagg acgacaatag cccaatcatg gcgttgaacg gcagcaagtg accgcgctgc
     3361 aaaattctga gcgccgcaat gagatacgga tttgccggcg cgaaattgac cagcaggaac
     3421 ccaactacga aacagagtgt agcagcgcgc atcaagttca ccggcttgct ttgcgcgagt
     3481 gcgggccaca ccatcacgcc tgccaccagc ccaagcgcag atcctggcgt cagccacagg
     3541 cccgggtcgc ctggaataaa caaggccatg gcggcaatgg ttttgaatgc caacgctgaa
     3601 agcataatca ccgtgaccgc cacggcagtc gcggcgaatg agcgcgtgat cgtcccaacc
     3661 agacaggcga taccaacgaa attaatcgcg gtgatgccgg cctcaagcag caagtagact
     3721 tccgcggaat aacgtaagcc ggaattcaac ttaaaaatgt gccttaggtc gccgttgccg
     3781 aatagcaaaa tatccgcgtt gaattgggta agtaaccaaa gaaagagcag caaaaacgcc
     3841 aggtcggtgc gtgcgccgcc ctgaaagtgc cgttcgcgca ggcgacgcag ttctccagac
     3901 agcagccagc gttgccccca ggcgtaggcg gccgctgctc cccataaggc gcctatcgaa
     3961 ttggtcagca cgtcgaggtt cgatgaaatg cgcgttggaa cgcaggcctg ggcgaattca
     4021 atggcaaggc tgagaagcac gcccgtgccg acggccaata ccattgcacg tagccgcggc
     4081 acgtacccca tccagaacag tgtcagcagc agtccaagcg gaaagtacgc aacaatgttt
     4141 accacaacgt ccgaggcaac tatgtacggc ggccatcccg caaacaagaa acgggcggct
     4201 tcgtaacctg gaatgcgcca atccgagaag ggatacaagc tggcgtaccc caccagcagc
     4261 aggtaagcta cagtcaggta ggcaaccagc ctggaattgc gcgcgtagcg cgcgggcgga
     4321 aaagcaagcg aaccgctcat gttgttggat tgcgcgctgg ggccatccat tcacgcggac
     4381 ggcgcagcat gggattctat gacatcttca atggcgatgc cgatggactc tgcgcgctgc
     4441 agcagctgag gctggccgaa ccgcgcgcag ccacgctggt caccggcgtc aaacgggata
     4501 tcgcgttgct tggtcaggta tcgcccaagg cgggcgacga gcttaccgtc ctggacgttt
     4561 cgctggattc gaatcgcgac gcgctgcttc gcaccttggc agttggagca catgtgcgct
     4621 atttcgatca ccatttcgcg ggagaaatcc cgactcatgc gttgctggaa acccacatcg
     4681 atatgtccgc gaccacttgc acgagcctca tcgttgacgg ttatttggac ggccgccatc
     4741 gcatgtgggc catcgtggct gctttcggcg ataacctgcc cgcgcaagct tacgcagcag
     4801 cggccgaact tgggatcggc agtgacgacg ccgaaacgct taaagacctc ggcgaaaacc
     4861 taaactacaa cgcttacggc gcgaatctgg ccgaacttca cttccatccc gccacgctgt
     4921 atctgcgcat gcggccctat gcggatccac ttgcattctg cgcgcacgcg cctgaactgg
     4981 atacgctgcg ccgtgcccgc gaagatgatt tgcggcacgc acgtgccttg ccattggaga
     5041 aggtgggcga gcatgcggtg ctggtctgct tgccgcaagc ggcatggagc cggcgcgtta
     5101 tcgggacttt cgcgaacgag ctggcgcggc agcatgccaa gcggattgtg gctttgctgg
     5161 tacgcaccgg cgcaggctat caggtcagtt tgcgcgcgcc ggaacaggga gaagcggcca
     5221 tgcatctgct ggcgcggcaa ttcgaatccg gaaatggcag ggctcgcgct gcaggtatcg
     5281 cgttcctgcc ggactcggag gtaaccagat tgcgtgcgct gttgaacaag ccgccaagcc
     5341 ccgaacccgc ctgaagtgaa aaggcgggga agccgtaggc ttgtgaacgt tattttgctt
     5401 ttatttcggt ccagatttta ttaagcagct ggcgctcctt tttgttcatg tctttgagct
     5461 gttccaatcg tttggccgac tgcgcatccg gaaacaccgc cttgatggac ttgatctccg
     5521 gcttgatgta ttgcatggcc tcggcattcg gattgccgga gcccgtgatg ttggtcaacg
     5581 ctgcggagtt cttgccctcc agcatgaaat tgatgaactg gtgagcaagg tcggcacgtg
     5641 gtgcggtttt cgcgatcacc atcgcgtcca gcgccagcac ggcgccttcc ttcggcagtg
     5701 catgacggat gcggaatttc tgcttggctt cctgcgcgcc agcatcggcc tggaaaatgt
     5761 cgttcgagta accgtgtacg acccagatat taccggtgga aagctccttg atgtatccct
     5821 gcgccatgaa agcggcccaa tacggcttgg ctttcaggat gacgtccttg gcctgattcc
     5881 aatgcgccgg atcgcggtcg ttcaccgagt agccgagata cttcagtgca gccgccatga
     5941 cttccgcctg gctgtccagc acagtgatct tcccttttat tttttgcagg atcttcggat
     6001 cgaaaattac cgcccatgag tccacggtga tgcccagttc cttcatcttc tgatcgttgt
     6061 agccgatcag cgtgatggac atcgcgtaag gaacggaata cttgttgccc ggatcgaacg
     6121 gggtattcag gtaagccggc atcagatttt tgaaattggg caatttgctc ttgtcgagtg
     6181 gctgcagcca gccggccttc gccatgcctg ccacataatt cgatgtgggc accatcacgt
     6241 cgtacccctt cgcacccgcg gccagcttgg cgaccagttc ttcattgtcg ccgtagaaat
     6301 cctgaacaac tttgcatttg cattgcgcct caaattcctt gaccgattcc ggggcaatgt
     6361 aatcattcca gttgtagaga tgcagttcat ccgcggcaag tgccggaagt gcgataaaca
     6421 gcaatagagg cagcagcttt ttcatggtgg gtctccgtga tttggcgaag cacgttgttc
     6481 ttggtcctgc ggtagcttag gcaaacgcgg ctggtgcgtc cagaaccatc gtttatcacg
     6541 gcttttttac tgggtacgca atgacccggg cgccagccgc gacgcaatca ggatcaatgc
     6601 cagtgtaagc gtcatcagca acgtggaaac agcattgact tcgggcgtga ccgcaatctt
     6661 caccatcgta taaatctgca gcggcaaggt ggtcgaacct acgcccgcag taaagaacgt
     6721 aatgacgaaa tcgtcgatcg aaagcgtaaa cgccatcaat gcgcccgcca caacgccggg
     6781 catgatcaat ggaagggtta ccagccggaa ggctgtccag ggtgtagccc ccagatcgcg
     6841 cgcggcttcg gtcaagcttt catccatgcc ggcaaggcgg gaacgaacaa caatcgccac
     6901 gaagccaatg cagaaggcga tgtgcgatag cacgatggaa accatgccga gcgaaatatt
     6961 cagcagtacg aaaaagatga gtagcgagac gcccatgagt atttcaggaa ttgcgatcgg
     7021 ggccaggatc agtactggca gcaatcgcgt gcggaagcga tacatcgcat agccagccat
     7081 cgtgcccaat atcgttgata cggcgcttgc cgtgataccg atcagcagcg aattccaggc
     7141 cgcatgcagc atcttttcgt tatggaacag tttctgatac caggacaggg taaaacctac
     7201 ccattcagca ttgagtctgg aatcgttgaa ggaatagacc accacgatag cgagcggcgc
     7261 gtagagaaat gcatacgcca gaatggcagc cgaccacagt aagactaccc gcttgttcac
     7321 gtacgcgccc gtttcgcaat ccaggcagcc gctccggcaa gcacgatcgc caggacggtc
     7381 aataccatcg acagtacgct accgaaaggc caatcgcgcg tgtccaggaa ctgctgcttg
     7441 atgacgtttc cgatcatgat gccttcggtg ccccccagga tatcgggaat ggcaaatatg
     7501 ccaagcgccg gaatgaagac gagcgctgcg ccggcataga cgcccggcag ggacaaggga
     7561 aaggttaccc gccagaaacg ccgccacgca ttggcgccca gatcctgcgc ggcatcgagc
     7621 agcgcgggat cgtgtttctc cagattggcg tacagcggca agaccatgaa gggcagatgc
     7681 acatagacca ggccgatgat caccgcaaaa ggcgtgtaga gcagcgtgag tggagccaaa
     7741 ccgaaccagc caacgatatg attcagcgcc gacacaaaca ctgattgtgg cccgaggatg
     7801 atcatccatg cgtacacccg aatcaaaaag ttcgaccaga acggcaggat aaccaatagc
     7861 accagcagat cgcgatgccg cttggagctg cgcgcaatca acatggcaag cgggtaggcg
     7921 atcagcaggc atgcgagggt tgtcaccccg gcatatgcga gcgatttgag gaacagctcc
     7981 gtataaagga aatcaccaaa gaaacgtcca tagctttccg tggtgagatc aatttcccgg
     8041 cttccggatt cgtcgttcag gagtggcgcc aggccgccga attcccccgg attgcgaaaa
     8101 gacgccagca ccatgatcag cacgggaatg gcgaaaaata caatcaagta ggcgaggggc
     8161 ggcccactga tcagccattt atgcaggcgg ttttgctgtg ccgccatggt tcagtcgttc
     8221 agaaaatggc cggcgtcatg gcgccacgca atttgtacct catcgcccgc ctcgaagaat
     8281 ttggtgcgac cggccgccga attcggcaac aaggcttcaa tgcgcatgcc atttgcaagt
     8341 tcgacgatgt agatggtcac atcgccgaga tacagcaggt cgtgcacctt gccggtaaag
     8401 cagttggcct cggcatccgc cggaaggtcg cgtccgatcg cgactttttc gggtcgcaac
     8461 gccagggcgc ctttcaatcc gggcgcatcg ccttccatat tgattgccac cacgtcgcca
     8521 ataccagata acgccacagt catacgctcg ccggaacaag ccttgatctg gccgtcgaga
     8581 agattgcact gaccgataaa gtcggcgacg aaacgattct tcggaaatcc gtagattttg
     8641 gaaggttcgt cgacttgttc gactctcccc tggttcatca cggcaatgcg atgcgacagc
     8701 gcgagggctt cgctttggtc gtgggtcaca tagacaaacg tgatgccgat ttccttttgc
     8761 agcgcgatta gttcgagctg cacgctttcc ttgagtttcg catccagtgc ggcaagcggc
     8821 tcatcgagca gaagcagttt cgggcgattg atcagcgcgc gggccacggc cacgcgctgc
     8881 ttctggccgc cggacagttc attcggatag cgtttcccca tcccggtcag acgcacatct
     8941 tccagggttt cctcgacacg ctgtgcgatt cgcgccgggt ccacatttgc catcttgagt
     9001 gggaatgcga cattctgttc cacgttcata tgcggaaaca gcgcgtagct ctggaatacg
     9061 gtgtggacgt tgcgggcttc gggcggaatc ccgaccatgt ccttgccatc cagcacgatg
     9121 ctgcctccat ccggcacgtc gaagccggcg atcatgcgca acagcgttgt tttcccgcag
     9181 ccggatggtc ccagcagagt gaagaattcg ccggcctcga tgttgaggct gacctgatcg
     9241 acggcagcga aggtaccgaa gcgccgcgtg acattgcgaa tttcgagaag cggcatcctg
     9301 ccggtccgag taaacgggca ttctagcaaa tggcatgcga cggtaaaccg tcacgaatgc
     9361 tttgcacgta gctaaacctt cgtcggccgc cattgcaacc gggcacaaca cgattgccgg
     9421 gcacccggtg ttacgattgg cacagcggcg accaattttt cggcgacgcg tgtgaaaaaa
     9481 ggtattccgt cagccggaga agaacgaaac acaatgaagg cagcggcaca gtctgcgccg
     9541 ggcggcgtgc gtgccccgga tgccaatcgc tggtggcaat taatcattgg catcatctgc
     9601 atgtcgatga ttgccaatct ccagtacggc tggacgctgt tcgttaaccc cctggacgac
     9661 aagttccaat ggggcaggac gtcgatccag gttgcgttca ccattttcat attggccgaa
     9721 acctggctgg taccgctcga gggttacctc gtggaccgct ttggcccggc attgatcacg
     9781 gtgagtgcgg gactgctggt cggcctgtcg tgggttctgg actcgctcgc ggactcgctt
     9841 gcgctgttgt attttgccgc cgcactcggc ggcataggcg ccggggccgt gtacggcact
     9901 tgcatcggcc aggcgctgaa gtggtttccc gatcggcgcg gtctcgcggc cggcctcacc
     9961 gccatgggat tcggcgcggg atcagcactc accgtgattc cgctcgcttc aatgatcaaa
    10021 tccagcggct accaggcgac cttcctctac ttcggcattg cgcagggact cgtcgtgttc
    10081 gcgctggggt ggcttttgaa gaaacccggt ccgggtcccg gcatcgcgca cggcattcgg
    10141 cctgcgccgg agcggcactt
//
LOCUS       DATMZQ010000256         3134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_549586, whole genome shotgun sequence.
ACCESSION   DATMZQ010000256 DATMZQ010000000
VERSION     DATMZQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549586"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VN496_08355"
     CDS             <1..243
                     /locus_tag="VN496_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17002.1"
                     /translation="GGNPNRMIPLNHNRDIALFDGRNLFAIGFRKTSAQYAYDPETHQ
                     QRPVAADPERMKDAIAVFQKAYEFYDKRRYHLRDGR"
     gene            389..841
                     /gene="rpsF"
                     /locus_tag="VN496_08360"
     CDS             389..841
                     /gene="rpsF"
                     /locus_tag="VN496_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXF17003.1"
                     /translation="MRHYEIVFIVHPDQSEQVPGMIERYRQMVTSRNGHIHRLEDWGR
                     RQLTFPIQKVHKAHYVLMNIECDQSTLDELEHAYKFNDAVVRHLIVNMTRAVTEPSPM
                     MKEEKSRTVVGEGARSEAPRPDHARGEGARSDTGAAQSADKPAPVQNA"
     gene            848..1144
                     /gene="priB"
                     /locus_tag="VN496_08365"
     CDS             848..1144
                     /gene="priB"
                     /locus_tag="VN496_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HXF17004.1"
                     /translation="MSRNTVILNGMLIGRDELRFTPAGTPALNFRIGHASEQIEAGHG
                     RSVKLEIDAVAIGEVAETLRKMPMEGEYLFKGFLALRNKLSRYPVLHVNSFELL"
     gene            1156..1437
                     /gene="rpsR"
                     /locus_tag="VN496_08370"
     CDS             1156..1437
                     /gene="rpsR"
                     /locus_tag="VN496_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXF17005.1"
                     /translation="MPRFGGSKGKGKDKGKERSNRPLFRRRKFCRFTAEGIKEVDYKD
                     VEILKDFINENGRIIPARITGTKTHFQRQLSVAIKRARYLALLPYTDLH"
     gene            1449..1904
                     /gene="rplI"
                     /locus_tag="VN496_08375"
     CDS             1449..1904
                     /gene="rplI"
                     /locus_tag="VN496_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXF17006.1"
                     /translation="MQIILMEKVHNLGQLGDVVKVKDGFARNFLIPQGKGKRATEKAI
                     EEFKARRTDLEAAHNKALALAQERASRLEGLMIQITQKAGVDGRLFGSVTNYDIAESL
                     KSQGHEVTKAMVRLPQGPLKQVGDYPITLDLNADVTTTITVSVLGDSSN"
     gene            2089..>3134
                     /locus_tag="VN496_08380"
     CDS             2089..>3134
                     /locus_tag="VN496_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HXF17007.1"
                     /translation="MADTQVELIKLPPHSVEAEQSVLGGLLLDNPAWDKVADMIGESD
                     FYRADHRLIYRHISKLIGGSRPADVITVSESLESTRELEGVGGLAYLGALAQNTPTAA
                     NIRRYAEIVRERAVMRKLAEVGTEIADTAYNPMGKEAGQLLDEAESKVFAISEEGARG
                     KQGFLDMQPLLTQVVERIDMLYNRDNPSDVTGLPTGFTDLDRMTSGLQPGDLVIVAGR
                     PSMGKTALAMNMAEHAALETGMPVGIFSMEMGASQLVMRMLGSVGRLDQHKVRTGRLA
                     DTDWRKLTDAVGRLNEAPIHIDETAALNALELRARARRLHRQYGKLGLIVVDYLQLMS
                     ASSQGENRATEISE"
BASE COUNT          743 a          868 c          920 g          603 t
ORIGIN      
        1 ggcggtaatc cgaaccgcat gatcccgctg aaccataatc gggacattgc gctgttcgac
       61 ggtcgcaatt tatttgcgat cggatttcgc aagacttctg cgcaatatgc ctacgatccc
      121 gaaacgcacc agcaaaggcc cgtagcggcc gatcccgagc gcatgaagga cgcaatcgcg
      181 gtttttcaga aggcatacga gttttacgac aagcgccgct accatctgcg cgacggacgt
      241 tgacgttgcc cctggcatgc ggcgcgattg atgttcaggg caaaatcggt ataatgccgg
      301 ccttccttgc cttaccgggt ccgcatcccg gaaggcttcc cgccccgtga ccgaaccact
      361 gaggggcctt acccataagg agacatgaat gcggcattac gagatcgttt tcatcgtcca
      421 tcccgaccag agcgaacagg tccccgggat gattgagcgc taccgccaga tggttacttc
      481 gcgcaacggc catattcacc ggctggaaga ctggggccgg cgtcaactga cctttccaat
      541 tcagaaagtc cacaaggcgc actacgtcct catgaatatc gagtgcgacc agtccacgct
      601 ggacgagctt gagcacgcct ataaattcaa tgacgcagtc gtgcggcatc tcatcgtcaa
      661 catgacgcgc gcggtgacgg aaccttctcc aatgatgaag gaagagaagt cccgcacggt
      721 cgtcggcgaa ggcgcacgca gcgaggctcc gcgaccggat catgctcgcg gggaaggtgc
      781 ccgttccgat accggcgcag cgcaaagcgc ggacaaacca gccccggtac agaacgccta
      841 gagtttagtg agccgcaaca cggttatcct gaacggcatg ttgattggac gcgatgaact
      901 tcgctttacc ccggcaggta cgccagcatt gaatttcagg atcggtcacg cttccgagca
      961 gatcgaggcc gggcacggca gaagcgtaaa gctggaaata gatgcggtgg cgatcggcga
     1021 agtggccgaa acgctgcgca agatgccgat ggaaggtgaa tacctgttca agggctttct
     1081 ggcgttacgc aataaactgt cgaggtaccc tgtgttgcac gtgaacagtt ttgaattgtt
     1141 atagaggata gacaaatgcc acgtttcgga ggcagtaaag gcaaaggcaa ggacaaaggc
     1201 aaggaacgca gcaatcgccc gctgttccgt cgccgtaaat tctgccgttt cacggcggaa
     1261 ggtatcaagg aagtcgatta caaggatgtg gaaatcctga aggatttcat caacgagaat
     1321 ggtcgcatta ttcctgcccg cattaccggc acgaagacac acttccagcg ccagttatcc
     1381 gtggcgatca agcgcgcgcg ctatctggcg ctgctgccgt acactgatct gcactgaagg
     1441 acactgacat gcagattatc ttgatggaaa aagtgcacaa ccttggccag cttggcgacg
     1501 tcgtcaaagt caaggatggc ttcgcccgca atttcctgat tccgcaaggc aagggcaagc
     1561 gtgcaaccga gaaggcaatc gaggaattca aagcgcgccg caccgacctg gaagctgcgc
     1621 ataacaaggc attggcattg gcgcaggaac gcgcgtccag gctggaaggc ctgatgatcc
     1681 agatcacgca gaaggcaggc gttgatggac ggctattcgg ttcggttacc aattatgaca
     1741 tcgccgagtc gctgaagagc caggggcatg aagtcaccaa agccatggtg cgcttgccgc
     1801 aggggccgct gaagcaggtg ggcgactatc cgattacgct cgatttgaac gcagacgtta
     1861 cgacgaccat cactgtttca gttctcggcg acagttcgaa ctgacgttgc aggtctgagg
     1921 caaaaaaaag ggctgtgaaa acagcccttt ttatttttct tcttgcgcgc caccgcggct
     1981 tacgaataat agctggcgaa gcaccgtgcg cgctgttcgt tcgtctcgat atcagatcaa
     2041 taatcacata agagatccat ggcgcagttc gcaacgcaac gcgtcggcat ggccgacacc
     2101 caggtggaac tcatcaagct gccgccgcat tccgtggagg cggagcagtc ggtgctgggg
     2161 ggactgctgc tcgacaaccc tgcctgggac aaagtagcgg acatgatcgg tgaatcggat
     2221 ttctatcgag ccgatcaccg cctcatttat cggcacatct ccaagctgat tggcggttcc
     2281 aggccggccg atgtcatcac cgtctccgag agtctggaga gcacgcgtga attggaaggc
     2341 gtcggcggcc tggcgtatct tggggcgctc gcgcagaaca cgcctactgc ggcgaacatc
     2401 cggcgttacg ccgaaatcgt gcgggaacgt gcggtcatgc gcaagcttgc cgaagtcggc
     2461 actgaaatcg ctgacaccgc ctacaacccg atgggcaagg aagctggcca gctgctggat
     2521 gaagcggaat ccaaagtgtt cgcgatctcg gaagagggcg cccgcggcaa gcaaggcttt
     2581 cttgatatgc agccactgct aacgcaagtc gtcgagcgca tcgacatgct gtacaaccgt
     2641 gacaatcctt ccgatgtcac cggtttaccg acggggttca cggaccttga ccgcatgaca
     2701 tcaggattgc aacctggaga tctggtcatc gttgcgggac gtccctcaat gggcaagacc
     2761 gcgctggcca tgaacatggc cgagcatgca gcgcttgaaa cgggtatgcc ggttggaatt
     2821 ttttcgatgg aaatgggcgc ttcgcaactg gtcatgcgca tgctcggcag cgtaggcagg
     2881 ctcgatcagc acaaggtgcg aactggccgg ctcgccgaca ctgattggcg caagctgacc
     2941 gacgcggtgg gccggctcaa cgaggcgcca atccacatcg acgaaacggc agcgctcaat
     3001 gcgcttgaac tgagggcgcg tgcgcggcga ttgcatcgcc aatacggcaa gctcggactc
     3061 atcgtcgtgg actacctgca gcttatgtcg gcgagttcac aaggcgaaaa ccgcgctaca
     3121 gaaatatcag aaat
//
LOCUS       DATMZQ010000257         5175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2619147, whole genome shotgun sequence.
ACCESSION   DATMZQ010000257 DATMZQ010000000
VERSION     DATMZQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619147"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN496_08385"
     CDS             complement(<1..524)
                     /locus_tag="VN496_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF17008.1"
                     /translation="MILHHRLSRPLPFLVLGVGVAVVSFAAILIRFAQVEGASSLAIA
                     AVRLTVAAAVLTPFVWLRSGGEIMRLGRRPLVLCMLSGILLAAHFWAWITSLEYTSVA
                     SSTALVTTNPLWVALVSAVVLREHPGAAGLAGIVLTLVGSMLVFAADASRAAAAGTAP
                     NLGNALALLGAISAS"
     gene            complement(521..955)
                     /locus_tag="VN496_08390"
     CDS             complement(521..955)
                     /locus_tag="VN496_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HXF17009.1"
                     /translation="MIVFDLTCDNSHPFEGWFGSADDFALQAKAGDIACPVCGSAEIT
                     RELSAPYVKTASESMAADALPAISVANAAQELKKKFIEHVLSNTEDVGRRFPEEARKI
                     FYKEAPERAIRGTASGQEVTELKEEGIDVMAIPAPSLPEKLH"
     gene            complement(1079..2335)
                     /gene="lysA"
                     /locus_tag="VN496_08395"
     CDS             complement(1079..2335)
                     /gene="lysA"
                     /locus_tag="VN496_08395"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXF17010.1"
                     /translation="MSAFSLHSGQLYVEKVALPEIAERFGTPCFVYSRAALEQAYLSY
                     DRAFGGRSHLICYAMKANSNLAILNLFARLGSGFDIVSGGELARVIAAGGSPGKTVFS
                     GVGKTRDEMRQALGAGVLCFNVESESELERLNDVARELRRKAPVSLRVNPDVDAKTHP
                     YISTGRKENKFGIAYGDALRIYRKARGLGHLHIVGIDCHIGSQLTELAPFIDALNKVL
                     ELVDKLSAEGIELKHVDLGGGLGIRYRDESPPLPDIYVKSLLKCLGERPQEILLEPGR
                     ALVGNAGLLLTRVEYLKHEDTRNFAVVDAAMNDLIRPALYDGYHEIRQVEPSTAAPKR
                     YQIVGPVCETADFLGNDRDLALKQGDLLAVMSAGAYGMSMSSNYNSRLRAAEVMVDGD
                     TVHLIRERDRVEQLFSAERMLMTNTA"
     gene            complement(2540..3175)
                     /locus_tag="VN496_08400"
     CDS             complement(2540..3175)
                     /locus_tag="VN496_08400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HXF17011.1"
                     /translation="MLTVDERGLTVSVPWRSSERRIAGAIYDAEAWVLKKLDVWSAYP
                     ARNQSWTDGDRVKYLGRDLSLHLYRDPTASLVTLQEPNRLHVTLADPTHKDGVKAVLV
                     NWYRRHAQSYFIDRIAAFAPRLDVPIPRLLLSNAQTRWGSCNAKREIRLNWRLIQASQ
                     STIDYVVVHELAHLIEMNHSRRFWKLVAGICPHYREACGELNRMGLYYMDI"
     gene            complement(3280..4008)
                     /locus_tag="VN496_08405"
     CDS             complement(3280..4008)
                     /locus_tag="VN496_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXF17012.1"
                     /translation="MSGLAALLRSVVYEVVRLAITVVFAIVSLCTFAFKPITRYRIIT
                     AWSHLVINLARVICGVHYRVIGLGNLPRGPCIVLSKHQSAWETLAYQVILPPQVWVLK
                     RELLRVPFFGWGLAMMSPIAINRGSAARALKQTLEQGKSRLADGWWIVIFPEGTRMPP
                     GERGRYHLGGAWLACKTGTPVLPIAHNAGSVWGKNAFIKYPGTITVSIGPVIHPAGMA
                     PDALNRKAEDWIENEVVRLGSARS"
     gene            complement(4005..4538)
                     /gene="gmhB"
                     /locus_tag="VN496_08410"
     CDS             complement(4005..4538)
                     /gene="gmhB"
                     /locus_tag="VN496_08410"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HXF17013.1"
                     /translation="MKLVILDRDGVINQDSDQFIKNTTEWKPIPGSLEAIAKLNHAGY
                     RVVIASNQSGIGRGLLDMGALNAINDRMYRALAQVGGRIDALFYCPHAAGDNCDCRKP
                     KPGMFIDIAQRFNVDLAGVPSIGDSLRDLQAAAAAGARPILVLTGKGAKTETAGGLPD
                     GTPVFADLAEAVRNIVA"
     gene            complement(4535..>5175)
                     /locus_tag="VN496_08415"
     CDS             complement(4535..>5175)
                     /locus_tag="VN496_08415"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXF17014.1"
                     /translation="LRRTALGVIRLLVERKLALSVHELINESFSVFPNGMLANTQADL
                     QLFIWERLRSYLRETGYSANEVDAVLSKNPAKIDQIPLWLAAVRAFASLPEAASLAAA
                     NKRVANILKQAEAKGEVFAKTQREDLKEPAEQKLFDALQDASADATLLFKEGDFTGYL
                     KTFAILKEPIDAFFDSVMVMAEDSSVRQNRLGLLADLRSEMNRIADISKLAA"
BASE COUNT         1122 a         1520 c         1421 g         1112 t
ORIGIN      
        1 gaggcagaaa ttgccccgag cagggccagc gcgttaccca ggttaggtgc cgttcctgca
       61 gccgccgcgc ggcttgcatc ggcagcaaat accagcatgc tacccacaag cgtcaacacg
      121 ataccggcca gtcctgcggc ccctggatgc tctcgcagaa cgacggcaga taccaaggcg
      181 acccacagcg gattcgtggt caccagtgca gtgctgctgg caaccgaggt atattccagc
      241 gacgtaatcc acgcccagaa atgcgccgcg agcaggatgc cggagagcat gcagagtaca
      301 agcggccgcc gccccaaacg cattatctcg ccgccggaac gcagccacac aaaaggagtc
      361 agcacagccg ctgcaaccgt tagccgcacg gcagcgatgg caagcgagga agcaccctct
      421 acctgcgcga aacgaatgag gattgcagcg aacgatacga ccgcgacgcc gacgccgaga
      481 acgagaaatg gcaggggccg ggaaagccga tgatgcagga tcagtgcagc ttttccggca
      541 aagaaggtgc agggatagcc atcacgtcga taccttcttc cttgagctca gtgacttcct
      601 gaccggacgc agttccgcgg attgcgcgtt ccggcgcctc cttgtagaag atcttgcgcg
      661 cttcttccgg aaaacgtcgg ccgacgtctt cggtattgct gaggacgtgc tcgatgaatt
      721 ttttcttgag ttcttgggcc gcgttagcga cagatatggc cggtaatgcg tcagccgcca
      781 tgctttcgga agcggtcttt acatatggag ccgagagttc gcgcgtgatt tctgcggatc
      841 cgcagaccgg gcatgcgatg tctccggctt ttgcctgcaa ggcgaagtcg tcggcagaac
      901 caaaccagcc ctcgaacggg tggctattgt cacatgtcaa atcaaaaaca atcatcggtt
      961 tcccctgcac caaccactaa tgcgctgcca tgcagtctag catccgtttg gctacacgcc
     1021 taaagataga ggtcgcatcc gctcaatagt tcaggcgcaa atccgatcgg ctgccatctt
     1081 aagcggtgtt tgtcatcaac atgcgttccg cgctgaacaa ctgctctaca cggtcccgct
     1141 cgcgaatgag atgtacggtg tcgccgtcga ccatgacttc ggccgctcgc aagcggctgt
     1201 tgtagttcga actcatgctc atgccgtagg cacccgcaga catgacagcc agaaggtcgc
     1261 cttgcttcaa tgcgagatca cgatcattgc ccagaaaatc tgccgtttcg cataccggac
     1321 cgacaatctg gtagcgcttc ggagcggcag ttgaaggctc aacttgccga atctcgtgat
     1381 acccatcgta taaggcgggg cgaatgaggt cgttcatcgc agcatccacc actgcaaagt
     1441 tacgcgtgtc ctcgtgcttc agatattcaa cacgggtcag cagcaatccc gcattgccga
     1501 ccaacgcccg ccccggctcc aatagtattt cctgcgggcg ttctccgagg catttcagta
     1561 acgacttcac gtaaatgtcc ggcagaggcg gtgactcgtc ccgatagcgg attcccagac
     1621 cgccgcccaa atcgacgtgc ttcaattcga tgccttcggc cgatagcttg tcgaccagtt
     1681 ccaacacttt attcaatgcg tcgatgaaag gcgcgagctc ggtcaattga gatcctatat
     1741 ggcaatcgat gccgacgatg tgcaaatgtc ccagtccgcg agccttacga tagattcgca
     1801 gtgcatcgcc ataagcgatt ccgaacttgt tttccttgcg tcctgtcgaa atatacggat
     1861 gcgtttttgc atcgacatcc ggatttacgc gcagactgac cggggccttg cggcgcagct
     1921 cgcgcgcgac atcgttcagt ctctccagtt cgctttccga ttcgacgttg aagcataaaa
     1981 cgcctgcgcc aagcgcctgg cgcatctcgt cgcgcgtctt gcccactcct gagaaaaccg
     2041 tcttgcccgg gctgccgcca gctgcaatca cgcgcgccag ctcaccaccc gaaacgatgt
     2101 caaatccgct tcccagccgg gcgaacaggt tcaggatggc gagattcgag ttggccttca
     2161 tcgcataaca gatcagatgt gaccgccctc cgaaagcccg gtcataactc aggtaagcct
     2221 gctcgagcgc ggcacgcgaa taaacaaagc aaggcgttcc aaaacgctcg gcgatctctg
     2281 gcagcgcaac tttttcgacg tacagctgcc ccgaatgcag ggaaaaggcg ctcacttctc
     2341 gatcgttcct tcttcggttt ttcttggttc ggtttttttt ggcttgtcgc cgtctttagg
     2401 caggtaaagc gggcctttct ggccgcaggc agatacgagc aaagcgaaga cgatcagaat
     2461 gcagatgcgg cgcatgggat atcgaaattt agcagtgagt ctagcacggg gcttgcgcat
     2521 caattaccgg cgagacgctc tagatatcca tgtagtagag ccccatgcgg ttgagctcgc
     2581 cgcaagcttc gcgataatgc ggacaaatcc ctgctaccag tttccagaaa cggcgtgagt
     2641 gattcatttc gatcaaatgc gccaactcat gtaccaccac atagtcaata gtcgactggc
     2701 tcgcctggat caggcgccag ttcaagcgta tttcccgttt ggcattgcaa ctgccccagc
     2761 gagtctgcgc atttgaaaga agcaaacgcg ggataggcac atcaagcctg ggtgcaaacg
     2821 ctgctatacg gtctatgaaa taggactgtg catggcggcg gtaccaattg accaggactg
     2881 cttttacgcc gtctttgtgg gtaggatcgg ccaatgtgac atgcaggcga tttggttcct
     2941 gcaaagtgac gagcgatgca gtcgggtcgc gatagagatg caaactgaga tcacggccaa
     3001 ggtatttgac tctgtcaccg tccgtccagg attgattgcg cgccgggtaa gcgctccaga
     3061 catccagttt cttgagcacc cacgcctcgg cgtcgtaaat ggcgccggcg atccggcgct
     3121 cggaactgcg ccagggcacg ctcaccgtca gaccgcgttc gtcaacggtc aacatgatgc
     3181 ttttgcgttg ttggctgcgc ttcaggaggt acgcgacatc ccggtcgcgc agccggatgc
     3241 tcttcggctc gcacaccatg gtaacgggaa acaggtcgct tatgagcgag cgctgccgag
     3301 gcggactact tcgttttcta tccagtcttc agctttccgg ttgagtgcat caggtgccat
     3361 ccccgcggga tgaatcaccg ggccgatgct cacggtgatg gtccccggat attttataaa
     3421 ggcatttttt ccccagacgc ttccggcatt atgcgcgatc ggcaaaactg gcgtgccagt
     3481 cttgcaagcc agccatgcgc ctcccagatg gtaacggcca cgttctcccg ggggcattcg
     3541 tgtgccttcc ggaaaaatga caatccacca cccgtccgcc aggcggctct tgccttgttc
     3601 cagcgtctgc ttcaaggcac gtgcagcgga accgcggttg attgcgatcg ggctcatcat
     3661 cgccaggccc cagccgaaga agggaacccg cagcagttcg cgcttgagca cccagacctg
     3721 cggtggaagg ataacctgat atgcgagcgt ttcccacgcc gactgatgct tggataggac
     3781 gatgcagggc cctcggggaa gattgcccag ccctattacg cggtagtgaa cgccgcagat
     3841 cacccttgcc aggttgatga cgagatgcga ccacgccgta atgatccggt agcgcgtaat
     3901 cggtttgaag gcaaaagtgc acaacgaaac gatggcgaat accacggtta tcgcgagcct
     3961 gacgacttcg tagacgaccg aacgcagcaa tgccgcgagt ccgctcatgc aacgatattg
     4021 cgtaccgcct cggcgaggtc agcaaaaacc ggggtaccgt ctggcaaccc tcccgcggtc
     4081 tctgttttcg cgcccttgcc ggtcagcacc agtatcggcc tcgcgccagc agcggccgct
     4141 gcctgaaggt cgcgcaagga atcgccgatg ctgggtacgc cagccagatc aacattaaat
     4201 ctttgcgcga tgtctatgaa catacctggc ttcggcttgc ggcagtcaca gttgtcaccc
     4261 gcggcgtgcg ggcaatagaa caaggcatcg atgcggccgc ccacttgggc cagcgcgcgg
     4321 tacatgcggt cgtttatcgc gttcaatgcg cccatatcca acaacccgcg cccgatgccg
     4381 gactggttgg atgcgattac aactcggtag cccgcgtggt ttagcttcgc gatcgcttca
     4441 aggcttcccg ggatcggttt ccactcggtc gtattcttga tgaattgatc gctatcctgg
     4501 ttgatgacgc cgtcacgatc gaggatgact agtttcatgc ggccagcttg gaaatgtcgg
     4561 cgatgcggtt catttcggat cgcaagtctg cgagtagacc caaccggttt tgacgtactg
     4621 agctgtcttc cgccatgacc ataaccgaat caaaaaacgc atcgatgggt tcctttagta
     4681 ttgcaaaggt tttcaggtaa ccggtgaagt ccccttcctt gaataataac gttgcatcgg
     4741 cggacgcgtc ttgcagcgcg tcgaacagtt tttgctcggc cggctccttt aaatcttcgc
     4801 gctgcgtctt ggcaaacacc tctcccttgg cttcggcttg cttcagaata ttggcaaccc
     4861 gcttgttggc cgctgcaaga ctcgcggctt ccggaagact ggcgaatgcg cgaactgccg
     4921 ccaaccagag cggaatttga tcgatcttcg ccggattctt gctgagaact gcatcgacct
     4981 cgtttgccga atatcccgtt tcacgtaagt agctgcgcaa gcgttcccag atgaagagtt
     5041 gcaagtccgc ctgtgtgttg gcgagcatgc cattcggaaa aaccgaaaaa gactcgttaa
     5101 tcagttcatg gaccgatagc gccagttttc tctcgaccaa cagtcgaatc acccccagtg
     5161 cagtccgtcg taatg
//
LOCUS       DATMZQ010000258         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4003430, whole genome shotgun sequence.
ACCESSION   DATMZQ010000258 DATMZQ010000000
VERSION     DATMZQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003430"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VN496_08420"
     CDS             <1..420
                     /locus_tag="VN496_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF17015.1"
                     /translation="NSGEGAIPRPFSCCLPYRLVENKMIASRSTFIMLAVTLITSILA
                     FRVFAQDPTDEAAARVNEKFGGNVKRLFSTKCSWCHQAYGMKQADGPKLAGTSKTLEQ
                     VMSQIKNGKSPMPGFKTQLKEEEVQALAEYIKALPAN"
     gene            complement(476..850)
                     /locus_tag="VN496_08425"
     CDS             complement(476..850)
                     /locus_tag="VN496_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17016.1"
                     /translation="MYGKAFIAVALVIFLSCAAADDAGNLAGSWKGPWYIGMSSGIAA
                     MEIAADGSGKITLTNLDEFGAQPVALTKQSFDGKRFGFTAIGANGAPLVMNLQLGEGG
                     KQLRGNGKHAGFGARMELQRTD"
     gene            1000..1587
                     /locus_tag="VN496_08430"
     CDS             1000..1587
                     /locus_tag="VN496_08430"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXF17017.1"
                     /translation="MNFCSTCGARVEHRVPPGDTLPRFVCAHCGIIHYENPKIVVGCI
                     PEWENRILLCRRAIEPRIGLWTVPAGFMENGETTAQGAQRETLEEANAKVEMLGLYAL
                     FNIPHINQVYMLFRARLLDLDFSAGAETLETMLFEEHQIPWDQLAFATVKRTLKLYFS
                     DRRDGSFRFHMGTIEPLPTSSRRVAADEERVKDEQ"
     gene            complement(1608..>2661)
                     /locus_tag="VN496_08435"
     CDS             complement(1608..>2661)
                     /locus_tag="VN496_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXF17018.1"
                     /translation="LQEGLRKTLVDTGSADNVVVTRRSSGTEVQSGIDRVQAAIVSSQ
                     PEIAYGPDGQSLVSKEMVVLITLQKRDSDKPSNVIIRGLDARGRQLRPQVHIVQGRMF
                     RPGSSEIIAGKGIAERFRGAGVGEQLRFGMRDWVVVGTFDAGKSGFDSEIWGDVDQLM
                     QAFRRPVYSSVIFKLNDPQRFDALKLRLESDPRLTLEAKRESVFYAEQSEVLSNFIGY
                     LGLTLSVIFSIGATIGAMITMYASVASRTAEIGTLRALGFRRGRILTVFLAEALLLGL
                     VGGIAGITLASFMQFLSISTMNWQSFAELAFSFTLNPVIISKSLVFALCMGLVGGFLP
                     ALRASRLNIVDALRAA"
BASE COUNT          611 a          805 c          716 g          529 t
ORIGIN      
        1 aattcgggcg agggagcgat ccctcgcccg ttttcttgtt gcctgcctta ccgccttgtg
       61 gagaacaaga tgatagcttc acgcagtaca ttcattatgc tggccgtcac gctgattacc
      121 agcattctgg ctttccgcgt gtttgcgcag gatccgactg acgaagccgc ggccagggtg
      181 aacgaaaaat ttggcggtaa cgtcaaaaga ctattttcga cgaaatgcag ctggtgccac
      241 caagcctacg gcatgaagca ggcggacggg ccaaagctcg ccggcacgag caagacgtta
      301 gagcaagtga tgtctcagat caaaaatgga aaatctccga tgcccggatt caaaacgcaa
      361 ctcaaggaag aagaagttca agcgctggcg gaatacatca aggcactgcc ggctaactag
      421 ccattatcat tatccaagca aaggtcggcc ttagccgcct aagttcgcga aaaagtcagt
      481 ccgttcgctg caattccatt ctggcgccaa atccggcatg cttgccgtta ccgcgcagct
      541 gcttgccgcc ctcccccaat tgaaggttca tgacgagcgg cgcgccattc gcgccaatgg
      601 cagtgaaacc aaaacgtttg ccgtcaaatg actgcttggt cagcgctacc ggttgcgcac
      661 cgaactcgtc caggttcgtc aaagtaattt ttcccgagcc atccgcggca atctccatgg
      721 ctgcgatgcc gctgctcatg ccgatatacc agggaccctt ccacgatccg gccagattgc
      781 cagcatcgtc agccgctgcg cagctcaaga aaatgaccag ggcgactgca ataaacgctt
      841 tgccgtacat gtgtcctccg gctatcgggt gcggcgtgcg ccgaaccatg catcattcta
      901 actgcgcgcc gccctcgcag cgcatcgttg aaaatatccg gaaagacaac ccgggacgcg
      961 cttatataat ggcctctgaa cagaccgatc gccatcccga tgaatttttg cagtacctgc
     1021 ggcgcgcgcg tggagcaccg tgtgcccccc ggcgataccc ttccccgctt tgtttgcgca
     1081 cattgcggga tcatccatta tgaaaacccc aaaatcgtag tggggtgcat cccggaatgg
     1141 gaaaaccgca tcctgctatg ccggcgcgcc atcgagccgc gcattggcct gtggacagtg
     1201 cccgcgggat tcatggaaaa cggcgagacg actgcacagg gtgcccagcg cgagacgctc
     1261 gaagaagcga acgcgaaagt cgagatgctg gggctttatg cattgttcaa catcccccac
     1321 atcaaccagg tctacatgct gtttcgtgcc cgactcctcg accttgattt cagcgcgggc
     1381 gcggaaacgc tggaaacgat gcttttcgaa gaacatcaga ttccctggga ccagctggct
     1441 tttgcgaccg tgaagcgaac gttgaagctc tacttcagcg accgccgcga tggaagcttt
     1501 cgcttccaca tgggcaccat agagccgtta ccaacgtcct cgcgccgcgt tgcggccgac
     1561 gaagaacggg tcaaagacga gcaataggta tgcggcggtt atttcgctca ggcggcccgc
     1621 aatgcgtcca cgatattcag gcgtgaagcg cgcagtgctg gtaaaaaacc gcccaccagg
     1681 cccatgcaaa gcgcgaatac cagtgatttg gaaatgatca ccgggttcag cgtgaacgag
     1741 aaggccagct cggcgaacga ctgccaattc atcgtggaga tgctcaggaa ctgcatgaaa
     1801 gaggcgagcg ttattccagc gatgccaccg accagtccca gcagcagcgc ttctgcaagg
     1861 aacacggtca gaatgcggcc cctgcggaaa ccgagtgcgc gcaaggtgcc gatttctgcc
     1921 gtgcggctcg ccaccgacgc atacatggtg atcatggcgc caatcgttgc tccgatagaa
     1981 aagattaccg agagcgtcaa acccagatag ccgatgaaat tggacagcac ctcggattgc
     2041 tccgcataaa agaccgattc gcgtttcgct tccaaagtca ggcgcggatc cgattccagt
     2101 cgcaacttca aagcgtcgaa gcgctgagga tcattcagtt tgaatatcac ggaagagtag
     2161 accggccggc ggaacgcctg catcagctga tcgacatcgc cccatatttc ggaatcgaag
     2221 ccgctcttgc cggcatcgaa cgttcccaca acgacccagt cgcgcatgcc aaatcgaagt
     2281 tgttcgccga ctcctgcgcc gcggaaccgc tcggcgatgc ccttgccggc gatgatttcc
     2341 gaagaacccg gccgaaacat ccgtccctgt acgatgtgca cctgcgggcg tagctgcctg
     2401 ccccgcgcgt ccaggccgcg aatgatgaca ttggacggct tgtccgaatc tcgcttctgc
     2461 agcgttatca gcactaccat ttccttcgac accaggcttt gtccatccgg gccatacgcg
     2521 atttccggct ggctcgaaac gattgcagcc tgtacgcgat ctatcccgct ctgcacttcc
     2581 gttcctgagg aacgccgggt gaccacaaca ttgtccgcgg agccggtgtc taccaatgtc
     2641 ttcctgagac cttcctgcag c
//
LOCUS       DATMZQ010000259         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_206946, whole genome shotgun sequence.
ACCESSION   DATMZQ010000259 DATMZQ010000000
VERSION     DATMZQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206946"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VN496_08440"
     CDS             complement(<1..743)
                     /locus_tag="VN496_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXF17019.1"
                     /translation="MKLMPTLPALLESYFTQRLMAQRQASPHTIASYRDTFRLLLNFA
                     QQQCGKSPSSLNLEDLDAPLISKFLDALEKQRGTSISSRNVRLTAVRSFFRYAAYEEP
                     AHSAQIQRVLAIQNKRQARPMISFLIREEIEALLAAPDQRTWNGRRDHALLRLAVQTG
                     LRLSEVTSLGREALVLGTGAHVQCHGKGRKTRRTPLTKQTAGVLKAWLKEPARGTSNV
                     LFPNVHGARLSSDAVQHLLAKYLAMAQQR"
     gene            complement(740..1468)
                     /locus_tag="VN496_08445"
     CDS             complement(740..1468)
                     /locus_tag="VN496_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXF17020.1"
                     /translation="MCVRVFARYCSAADPRTEVPPLGILPFHPQRATPYLYSEEEIRR
                     LMEAARCLEPSGGLRGLTYHCLFGLLTVTGLRISEALALTREDVDLRNGLLTIRGAKF
                     GKTRLVPLHPSACRALSRYAQQRDELLSPPRATNFLLCASGRPPEVSTVRRVFYQLSR
                     QTGLRGPSDHRGPRLQDFRHRCATEILLKWYQAGEDVERRLPVLSTFLGHGHISETFW
                     YLSGSSELLEQAARRLERRWEVQP"
     gene            complement(1667..>2731)
                     /locus_tag="VN496_08450"
     CDS             complement(1667..>2731)
                     /locus_tag="VN496_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXF17021.1"
                     /translation="RLDVDDVDERTVARYLQSRRCFVNGYRGAASVPYKFLGLLRDQG
                     MVNDRSMPVAVDACEVAIDNFKQYLSQERGLSLSIQYNYARFADRFLRERFGRGSVQF
                     SKLSATDITEFVRRHAHKGSACSARHMVGSLRAFLRYLRYQGEITTDLAACVPKVANW
                     SHSALPRFLQPGQVQKVLDHCDRRSSVGLRNYAILLLLARLGLRACEIVGMTLDDIDW
                     EASHLMVRGKGGQRAQMPLPAEVGRAIAVYLRKGRPSCASRHLFIREHAPRVGFANSA
                     AVSTLVQRALADAAVNSPHRGAYVFRHSLATEMLRQGASLGEIGQLLRHAHPDTTQIY
                     AKVNVQALRPLALRWPGGGR"
BASE COUNT          604 a          825 c          766 g          536 t
ORIGIN      
        1 caccgctgct gagccatagc gaggtatttc gcaaggaggt gctgcacggc atcggaacta
       61 agccgcgctc catgaacgtt gggaaataat acgttgctag ttccccgcgc cggttctttc
      121 aaccaggctt tcaaaacgcc agcggtttgt ttggtcaaag gcgtgcgcct ggtcttccgt
      181 cctttaccat ggcactgcac atgcgcgccg gtgcccaaca ccagagcctc tcgaccaagg
      241 ctcgttacct ccgatagacg tagaccggtc tgcacggcta accgcagcag cgcatggtcg
      301 cgccggccat tccaggtacg ctgatccggc gccgccagca gcgcctcgat ctcctcccgt
      361 attagaaagc tgatcatcgg acgtgcctga cgcttgtttt gaatggccaa aacgcgctga
      421 atctgcgcgg agtgggcggg ctcttcgtac gcggcatagc ggaagaaaga acggacggcc
      481 gtcagccgca cattgcgact gctgattgag gtaccgcgtt gcttctccaa agcatcgagg
      541 aacttactga tcaaaggcgc atccagatcc tcaagattca agctggaggg cgacttgccg
      601 cactgttgtt gtgcaaagtt caacagtaaa cggaacgtgt cccgataaga ggcaatcgta
      661 tgtgggctgg cttgtcgttg agccatcaaa cgctgcgtga agtaactctc cagcaaggcc
      721 ggcagggtgg gcattagctt catggttgca cctcccagcg acgctccaat cgtcgagccg
      781 cctgctccaa gagctcggac gaccctgaca agtaccagaa tgtctcgctg atatgcccgt
      841 ggcctaaaaa tgtggatagt accggcaatc ggcgctcaac atcctcgccc gcctgatacc
      901 acttgagcaa gatctccgtc gcgcagcgat ggcgaaagtc ttgcaggcga ggcccgcggt
      961 gatccgacgg accccgcagc cccgtttgac gcgagagttg ataaaagaca cgacgaaccg
     1021 tggagacttc cggcggtcgg ccgctggcac agagcagaaa gttggtagca cgcggcggac
     1081 ttaatagttc atcccgctgt tgcgcgtagc gcgaaagggc acggcaggcc gaaggatgta
     1141 aaggcaccag ccgggttttg ccgaattttg cgccgcgtat cgtgagcaga ccgtttcgca
     1201 gatcaacatc ttcacgcgtg agcgcgagag cttcactgat acgcaaaccg gtgactgtga
     1261 ggagtccgaa caggcagtgg taagttaacc cccgtagacc gccacttggc tccagacatc
     1321 gggccgcctc catcagccgc cgaatttcct cctcgctgta aaggtagggt gtggcccgtt
     1381 gcgggtgaaa cggtaaaatg cccagcggag gaacctccgt gcgcggatcc gcagcgctgc
     1441 agtagcgcgc aaaaacgcgt acgcacatca gccgttcggc ccaggtggcg cgttgcgcgt
     1501 cggccggctc cgttgcccac tttagcgcca gcttggttgt aatgtaggag gctttttacc
     1561 tccatgaagg aaacgaagcg tttcaaacgc cgctcctcat gccgcatctt gaaaccgagg
     1621 tcccgccgca tccgaatata gtcctgcgcc gcttgtttga gggatttcat cgcccacccc
     1681 ccggccagcg tagtgccaac ggacgcaatg cctggacatt gaccttcgcg taaatctggg
     1741 ttgtgtccgg atgtgcatga cgcaacagct gtccgatctc gcccagggac gctccctgcc
     1801 gaagcatctc cgtggccagc gagtgtctga atacgtaggc acccctgtgc ggcgagttta
     1861 cggcagcatc agctaaggcg cgttggacaa gtgttgagac agcggcagaa ttagcaaaac
     1921 caacccgcgg tgcatgctca cgaataaata gatgtcggct tgcacagctc ggtcgtcctt
     1981 tcctcaggta aacggcgatc gctcggccga cttccgcagg caacggcatt tgagcccgct
     2041 gtccgccctt gccgcgcacc atcagatggc tggcctccca atcgatgtca tcgagcgtca
     2101 tgccaactat ttcgcacgcc cgcaaaccca gtcgtgccag cagtagcagg atcgcatagt
     2161 tgcgtaggcc cacggagctg cggcgatcgc agtgatccag cactttctgt acttgtcctg
     2221 gctgcaagaa cctgggcaac gccgaatgcg accagttcgc caccttgggc acgcaagcgg
     2281 caaggtcagt cgtaatctcg ccttgatatc gcaggtatcg cagaaatgct cggagcgagc
     2341 cgaccatgtg tcgcgcactg cacgcgctgc ctttgtgagc gtgacgtcga acaaactccg
     2401 tgatatctgt ggcggaaagc ttcgagaact ggacagaacc acggccaaat cgctcccgaa
     2461 ggaaccgatc tgcgaaacgc gcgtaattgt actgaatcga tagcgataac ccccgctctt
     2521 gcgacagata ttgcttaaag ttgtcgatcg caacttcgca cgcatcaacc gcgaccggca
     2581 ttgacctgtc attaaccatc ccctgatcac gcaacaagcc gagaaacttg tatggaacac
     2641 tggccgcccc gcgatatcca ttcacaaagc atcgacggga ctgaagataa cgcgccacag
     2701 tgcgctcatc gacatcgtca acatcgagcc t
//
LOCUS       DATMZQ010000260        10899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4938742, whole genome shotgun sequence.
ACCESSION   DATMZQ010000260 DATMZQ010000000
VERSION     DATMZQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4938742"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VN496_08455"
     CDS             complement(<1..777)
                     /locus_tag="VN496_08455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXF17022.1"
                     /translation="MKFLTVLAAFLVSNIVLADELPDLGDVSQTVLSPVQERKLGEGI
                     MAQIRPNPAYLDDPEVTDYLNTIGYRLVANSPDPAGPFEFFAIEDNSINAFALPGGFV
                     GVHSGLLLAAQSESELASVLAHEIGHVTQRHIARLIAAQQRAGLASMAALAVAILAAR
                     SNSQVSAAAIAAAQAGAIQSQLSFTREHEREADRIGLQILQKSGFDVHAMPVFFERLQ
                     KATRIYETGAPSYLRTHPLTFERIADIQSRIENIPFKQVPD"
     gene            850..1335
                     /gene="moaC"
                     /locus_tag="VN496_08460"
     CDS             850..1335
                     /gene="moaC"
                     /locus_tag="VN496_08460"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXF17023.1"
                     /translation="MGELTHFDSTGQAHMVDVAAKPDTHRVAIAIGRIVMRPETLQKI
                     KEGSASKGDVLGVARVAAIQGAKRASELIPLCHPISLSAVNIEFNVLDKDNTVECFAR
                     TETVGRTGVEMEALTAVSVALLTIYDMCKAMDRGMRVEGVRLIEKRGGKSGHWKANSH
                     S"
     gene            1453..1941
                     /gene="soxY"
                     /locus_tag="VN496_08465"
     CDS             1453..1941
                     /gene="soxY"
                     /locus_tag="VN496_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HXF17024.1"
                     /translation="METKILVRLSRRTFLRSLAIGGGYLCVFSAGWLRPVKLFAAEWN
                     QAAFEAKVLADTLKGIGALSVSDSDQIQLKAPEIAENGAIVPVEITSKIPGTQAIYII
                     ADKNPQPLVAIFELEPNLEPYISTRIKMGESSKVRVLIRAGGKFYMATQEVKVTIGGC
                     GG"
     gene            1968..2282
                     /gene="soxZ"
                     /locus_tag="VN496_08470"
     CDS             1968..2282
                     /gene="soxZ"
                     /locus_tag="VN496_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HXF17025.1"
                     /translation="MADTMKIRAQLQGDVADVKVLMFHPMETGLRKDPISGEIIPLHF
                     IQRVIAALNGKTVMDAQWSRAVSKNPFVNFRIRGARAGDKVSVSWEDNMGAQASIETM
                     LS"
     gene            2401..4077
                     /gene="soxB"
                     /locus_tag="VN496_08475"
     CDS             2401..4077
                     /gene="soxB"
                     /locus_tag="VN496_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HXF17026.1"
                     /translation="MHRREFLQALALATISGTLPTSALGGDSASLYRLPERKRVASLI
                     HLTDCHAQLLPTYHREPSINIGVGDTPAQRTHLTGESLLRTYGLRRGSRLAHALSCVD
                     FEELARRYGRMGGFAHLATLVKLIESQRPGAMLVDGGDTWQGSATALWTRGADMVAAS
                     KALGVKVMTGHWEFTLGAERVTELIQRDMRGSIDFVAHNVFTRDFGDRVFLPYVIKDV
                     AGIEIAIIGQAYPYTPIAHPKSLTPDWTFGIHEQSLRDTIATARKAGAAAVVLLSHNG
                     VDVDLKLASRVEGLAAILGGHTHDPLPKPVLVKNNAGVTLVANGGSNGKFLGVLDIVS
                     ARGKMSCHYSLLPVFSNLLKPDDRMSSLIEELRQPFQPSLSKALTVTDDLLYRRGNFN
                     GSFDEIILEALQQSQDAEIALSPGFRWGTTLLPGSPVTVEDVMAQTAITYPGITVRNL
                     TGNEMKLALEDIADNLFHPDPYYRQGGDMVRTGGLTYTLNPRAAAGERISNMQLKKKR
                     IEADRAYRVAGWASVADNEPVGKPVWEVLRDYLSTTSSIHLESINVPTLL"
     gene            complement(4099..5547)
                     /locus_tag="VN496_08480"
     CDS             complement(4099..5547)
                     /locus_tag="VN496_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXF17027.1"
                     /translation="MTTTDLHTSPGPLFPALAVIGIYLILAATWMPGVAPRLYDDARY
                     VELGMLAIVMVLLSRPAVAQSLIISWNSLATSSRSLLLIFIVFGIASALISGARNLGL
                     LEIALIAQLILLSAVVAGAVRSGRADADKLLCVSIFAGAALCGLKFWVTYIQYAWEGK
                     SFSWVSPFLDFANVRFFSQYQAYTLFLVTLPVFLFPANKVWRLFFFLVAANFWAMHWM
                     VGTRAAWLGYIVALAVVLTFMPSCRTVWLRWHAGAMIAGGLICYSFSTFINSRAVTDA
                     VPGIGSIVERDYGSINERIALARIALNDVQSHPLFGVGPGQFGLRPYAINAAHPHNVP
                     LQLLAEYGLVAGAAGIWLGILLCVFAVRALRNQSPGANHAVAATTVAGLLMGVTDALF
                     SGNLIMPQSQVLFFVLAGWIVGRCFLVSRPIYTAGTLYRSQKLVIVSAGLFAAAITTV
                     LALEYLPLARELPVWLPRWNPHFWQYGRVYNW"
     gene            complement(5680..6195)
                     /locus_tag="VN496_08485"
     CDS             complement(5680..6195)
                     /locus_tag="VN496_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HXF17028.1"
                     /translation="MKAVQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAKMSEAI
                     LAASACRTSITETVQSASTLPAAGLWGCESSSTVAQVSKYVTSIKTSSKGEIRVQVTG
                     INATNVDGNAVILQPSNSATTAVAPAVGDAVSSWLCGPDPANAKDISKFLPGSCRAAI
                     TVYSGTFASST"
     gene            complement(6481..8082)
                     /locus_tag="VN496_08490"
     CDS             complement(6481..8082)
                     /locus_tag="VN496_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXF17029.1"
                     /translation="MEELNQVGISLSRVKDTNRLLEAILLAAKKITNADGGTLYRVDA
                     EKQLVHFEIVRTDSLDIAMGGTTGVEVPFYPVRLYDDEGQPNNSMVVAYSVLRDETVN
                     ITDAYAAKGFDFTGTKNFDGKTGYRSISFLTVPMKNHEDEIIGVLQLINAKDRHTGET
                     VSFSEADQRLAESLASQAAVALTNRQLISQLEGLFESFINLINAAIDDKSPYTGGHCQ
                     RVPMLTMMLAQAADEVDHGPLADFKMSDKDRYELKIAGLLHDCGKITTPVHVVDKETK
                     LQTIFDRITLIDTRFEILKRDADITRFKAKLNAAGNAAGEWEKADRALKAQLREIEED
                     RQFLRFCNFGSEAMREEDQERVRVISKKYRWRDVEGREVDFLTADEVGNLTIRSGTLT
                     QEERQIINHHIEVTIKMLESLPWPKHLKNVAEYAGGHHERMDGKGYPRGLTRDRMSVP
                     ARVMGIADIFEALTAKDRPYKKGKTLTESLQILGRFKVDGHIDPDLFDIFVSKKVYLR
                     YAEQFLDPDQIDEVDEAALPGYQHR"
     gene            complement(8161..10542)
                     /locus_tag="VN496_08495"
     CDS             complement(8161..10542)
                     /locus_tag="VN496_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXF17030.1"
                     /translation="MSTVFQSKPQAGQNLAEMSHKLEFQKKLQAVTNKIHATSNIDEI
                     MLELSQDICSLFEAERLTIYVLSEDRASIISKVKTGLSSFKDLKLPVNEQSIAGFVAL
                     SKKLINIRDVYDDNELRVHNPNLKFLKEVDKRTGFRTKQMLVAPVVDSAASELVGVIQ
                     VINSKSGHAFSTIAEEGATHLCETLEIALRQRSRPTAMLRGKYDYLVTNAVLSAEEME
                     LATRSARRKNLDIEDVLIDEFQVKPAALGEALSHFFKVSYEPFRQDRVKPLDLLKNLK
                     RDYVESSLWLPVEDVKEGLMVLTTDPEKVRASKVVSNIFPKHKVVYKVCTHREFGATL
                     DQFFGADSGGSSSIGELLSTMDEEGDEEGGASTDDASLAQDNELVKLVNKIIVDAYHQ
                     GASDIHIEPYPGKGKTEVRFRKDGTLHNYISVPASYRNAIAARLKVMCDLDISEKRKP
                     QDGKIKFKKFGPLDIELRVATIPSAGGVEDIVMRILAAGEPIPIDKLGLSQRNVGLLK
                     ETVSKPYGLFFVCGPTGSGKTTTLHSVLGYLNTPETKIWTAEDPVEITQKGLRQVQMN
                     PKAGLTFAVAMKAFLRADPDIIMVGEMRDKETTATGIEASLTGHLVFATLHTNSAPES
                     IIRLLDMGMDPFNFADALLGVLAQRLAKRLCSKCKTPHVATQEELKAILEEYSMELRN
                     TENWKRDPKAAYEAVYREWAKMFADDKGHFTLFTPVGCDACGGTGYKGRVGLHELLVG
                     TDTVKKHIQEHARVAEIVATALAEGMRTLKQDGIEKVLQGITDMLQVRAVCIK"
     gene            complement(10627..>10899)
                     /locus_tag="VN496_08500"
     CDS             complement(10627..>10899)
                     /locus_tag="VN496_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HXF17031.1"
                     /translation="ALWDEVNKISNLRYLVIETAFPNMEKELAIASKHLCPSMLAEEL
                     AKLRRPTRVFITHLKPGAGTLTMQEVEECAGRYNPRMLSNGQVFKF"
BASE COUNT         2549 a         2898 c         2885 g         2567 t
ORIGIN      
        1 gtcgggcacc tgcttgaaag gtatattctc gatgcggctt tgaatatctg cgatgcgctc
       61 gaacgtaagc gggtgtgtcc gcagataaga cggcgcgccg gtttcatata tacgagtagc
      121 tttctgaaga cgctcgaaaa acactggcat cgcgtgcacg tcaaatccag acttctgtag
      181 gatctgcagt ccgatgcggt cggcttctcg ctcgtgctca cgcgtaaaac tgagttgaga
      241 ttggatcgcg cctgcctggg cggccgcaat ggcggccgct gatacctgtg aattcgaacg
      301 tgcagcgaga atagcaactg ccagtgccgc catggaggcc aggccggctc gttgctgtgc
      361 tgcgattagc cgcgcaatgt ggcgttgtgt tacgtgcccg atttcgtgcg ccaatacgct
      421 agccagttct gattcacttt gtgccgcgag aagcagcccg gaatgcacac cgacgaaccc
      481 tccgggcaaa gcaaaagcgt taatgctgtt gtcttcaatt gcaaagaact cgaaaggacc
      541 ggcgggatcc ggactattcg caaccaggcg gtaccctatc gtattgagat agtcggttac
      601 ctccggatcg tccagataag ctggattcgg acggatctgg gccatgatac cttcgccgag
      661 cttgcgctct tgtacaggcg acagcaccgt ttgggacaca tctccgaggt cgggcaactc
      721 gtccgccagc acaatgttcg agaccagaaa agccgccagg actgtaagga atttcatggg
      781 tgctatgata acgctaccgc aggccgagtt ccgtgggacc agcccgctta cctacttcgg
      841 cacacgacta tgggcgaact aacccacttc gattcgacgg gccaggcaca tatggttgac
      901 gtagcggcaa agcccgatac gcatcgtgtt gccatcgcga ttggccgcat cgtaatgcgt
      961 ccggagacgt tacaaaagat aaaggaaggt tctgcgagca agggagatgt ccttggcgtc
     1021 gcgcgtgtcg cggcaatcca aggcgcgaag cgcgcatcgg aactaatacc gctctgtcat
     1081 ccgatatcgc ttagcgctgt gaatatagaa ttcaacgtgc tggacaaaga caacaccgtg
     1141 gaatgtttcg ctcgcaccga aacggttggg cggactggtg tcgagatgga agccctgacg
     1201 gcggtaagcg ttgcattgct taccatctat gacatgtgca aggccatgga tcggggtatg
     1261 cgcgttgaag gtgtgcggct gatagaaaag cgcggcggca agtcgggcca ttggaaagca
     1321 aactcgcact cttgatcgcg ccagaaccgt ttgccgtatt tttggtctca gctttcgaga
     1381 ccagccggat gcccggtgta acgtcggaag ggaagattgg ttgttggtta cttttgtagt
     1441 caataggccg ccatggaaac aaaaatacta gtgcggctgt cacgcagaac atttctgcgt
     1501 agtttggcga ttggtggcgg ctatctctgc gtcttttcgg caggatggct gcggccagtt
     1561 aagttattcg ccgcagaatg gaaccaggca gctttcgaag caaaagttct ggctgatact
     1621 ttaaaaggca tcggcgcgtt gtcggtctct gattccgacc agattcagct gaaggctccg
     1681 gaaatcgccg aaaatggggc aatcgttcca gtggaaatca cgagcaagat tcccggtacg
     1741 caggcgatat acatcattgc tgacaaaaat ccccagccgc tggtagctat atttgagctg
     1801 gaacccaatc tggagcctta tatttccact cgcatcaaaa tgggcgaatc atcgaaagtc
     1861 agggttctga ttcgggctgg cggcaaattt tatatggcga ctcaggaagt caaagtgacg
     1921 atcggcgggt gcggcggcta gcgttcgcga aaggggaaaa gagcgagatg gccgatacca
     1981 tgaaaatccg agcccagctt cagggcgacg tagcggatgt aaaagtattg atgttccacc
     2041 ccatggagac tggactgcgc aaggacccga tctcagggga aattatccca ctgcacttta
     2101 ttcagcgcgt catcgcggca ctgaatggca aaacagttat ggatgcacag tggagccggg
     2161 cagtgtcaaa aaaccctttc gttaattttc ggatacgcgg cgccagagca ggtgataagg
     2221 tttcagtcag ttgggaagat aacatgggcg cgcaggcgag catcgagacg atgctcagtt
     2281 gaatcgattg tcgcgccaca gtatttgccc cgcttcaggt ggcaatagag gcggctctga
     2341 aaatcctcgt attgccattc acactcgccg caagccggct taacttattg cgcgttatcg
     2401 atgcaccgcc gtgaatttct gcaagcgctg gcgcttgcca cgatatccgg gactttgccg
     2461 acctcggctt tgggcggcga ctcggcgtca ctttaccgtc ttcctgagcg caagcgagtc
     2521 gcaagcctga tacatctgac agattgccac gcacaattgc tgccgactta ccatcgagag
     2581 ccaagcatca atatcggcgt gggcgatacg ccagcccagc ggactcacct gaccggcgaa
     2641 tctttgctcc gtacttatgg gttacgccgt ggctcacggc tggcacacgc gctgagctgt
     2701 gtagattttg aagagctggc gaggcgatat ggccggatgg gcggattcgc gcacctggcg
     2761 accctggtca agctcattga gtctcagagg ccgggcgcaa tgctcgtgga tggtggagac
     2821 acctggcagg gctccgcgac tgcattatgg acgcgagggg cagacatggt tgccgccagc
     2881 aaggcgcttg gcgtgaaagt catgacaggc cattgggaat ttacccttgg cgccgaaaga
     2941 gtgacagagt taatacagcg cgacatgcgt ggctcaattg actttgtcgc gcataatgtt
     3001 ttcacgcgcg actttggcga tcgcgtgttc ttgccttatg taatcaagga tgtcgctgga
     3061 atcgagatcg cgattatcgg acaagcctac ccctacacgc ccatcgcaca ccccaaaagc
     3121 ctgactccag actggacttt cggtattcac gaacaatcgt tacgcgatac gatcgcgaca
     3181 gctcgcaaag ctggcgcagc ggcagttgtg ttgctatcgc acaacggagt cgatgtagac
     3241 ctcaaactcg caagtcgcgt tgaaggtttg gcagcgattc tcggtggcca cacccatgat
     3301 ccactgccga aaccagtcct cgtcaagaac aatgcgggcg tcacgctggt agccaacggc
     3361 ggatcgaacg gaaaattcct tggcgtattg gatattgtgt ccgcacgcgg caagatgagt
     3421 tgccactatt cgcttttgcc ggttttttca aatctgctaa aaccggatga tcggatgtcg
     3481 tccttgattg aagaattgag acagcctttc caaccctccc tgagtaaggc gcttacggtt
     3541 accgatgatt tgctttaccg acgcggtaat ttcaatggct ctttcgatga aataattttg
     3601 gaggctttac aacaatccca ggatgcggaa attgcgctct ctccggggtt tcgatggggt
     3661 accaccctgt tgcccggctc accggtgact gttgaagatg tcatggcgca aacggcaatt
     3721 acttatcccg gcatcaccgt gcgcaatctc acaggaaatg aaatgaaact tgcgctggaa
     3781 gatatcgccg acaacctttt ccatccagat ccctattacc gccagggcgg cgacatggta
     3841 agaaccggcg gattgaccta tacactgaat ccgcgtgccg cagccggtga acgaatcagc
     3901 aatatgcaat tgaagaagaa acgcatcgaa gcagatcgcg cttaccgagt cgccggttgg
     3961 gcatctgttg cggataacga accggttggc aaaccggtat gggaggtgtt gcgggactat
     4021 ctgtctacta cgtcgagcat acatcttgaa tcaatcaacg tgccaacgtt gctatagcct
     4081 tccgcgacta tggtctcatc accagttgta aacgcgcccg tattgccaga aatgcgggtt
     4141 ccagcgcggc agccaaaccg gcagttcgcg agccagggga aggtattcga gtgcgagtac
     4201 cgtggtaatt gccgcagcga aaagccccgc agaaacaatc acgagtttct gcgaacgata
     4261 cagcgtcccc gcagtataga tggggcgaga aactaaaaaa cagcgaccga cgatccatcc
     4321 cgccaacaca aaaaataaca cttggctttg gggcatgatc aaattaccgc tgaacaatgc
     4381 gtccgtgacg cccattaata ggccagcaac ggtggtggct gcaactgcat gattggcgcc
     4441 gggcgactgg tttcgtaaag ctcgaacggc aaatacgcag agcaggatac ccagccatat
     4501 tccagccgct ccagcaacca aaccatattc ggcaagcaat tgcagcggca cgttatgcgg
     4561 gtgcgccgca ttaattgcat acggccgcag tccaaactgc ccggggccca ccccaaacaa
     4621 ggggtgactt tgcacatcat tcagcgctat acgagcaagt gcgattctct cattgatgct
     4681 gccgtagtcg cgttcaacta tcgaccctat gccaggaacg gcgtcggtca cagctcggga
     4741 attgatgaac gttgaaaatg agtagcagat aagcccgccg gcgatcatcg cgcccgcatg
     4801 ccatcgcaac caaactgttc tgcacgaggg catgaaggtt agaaccaccg caagcgcgac
     4861 tatgtatccc agccaggccg cacgggttcc aaccatccag tgcattgccc agaaatttgc
     4921 agcgaccagg aagaaaaaga gacgccagac tttgttagcg ggaaacagaa agaccggcaa
     4981 ggtaaccaag aacaaagtgt aagcttggta ttgactaaag aaccgtacat tcgcgaaatc
     5041 caggaacggg ctgacccagg agaaggattt gccttcccat gcatattgaa tgtaggtaac
     5101 ccaaaattta agtccgcaca gtgcggcacc ggcaaaaatg gaaacgcaga gcagcttgtc
     5161 cgcgtccgca cgtccgctgc gtacagcacc tgcgacgaca gcactcaaca gaatcagttg
     5221 agcgatcaat gcgatctcga gcaatcccag attccgggca ccggatatca gcgcgctcgc
     5281 tatgccgaat acgataaata tcaatagaag gctgcgcgat gaggtggcaa gagaattcca
     5341 tgaaatgatt agcgattgcg cgacggcggg acgagacaac aacaccataa caatggccag
     5401 cataccgagc tctacgtaac gcgcatcgtc atagagtctg ggggccacgc cgggcatcca
     5461 ggtagcagcc agaataaggt agataccgat cacggccagc gcgggaaaca atggtccggg
     5521 gctggtatgt aaatcggtag ttgtcattgc tttacgcaat ttcctgatct gcgcgcggga
     5581 ctgtgattct gcttggccaa aaaagaaggc gccttttggc gccttctttt tgaacgatgc
     5641 gatcagaagc gataagccat ctcctgacct gtcaaagtat caagtactgg acgcaaaggt
     5701 accgctgtag accgtaatcg cggcacggca agagcccggc aagaacttcg aaatatcttt
     5761 cgcgttcgcc ggatccggac cgcacaacca gctggatact gcatcaccaa ctgccggagc
     5821 aaccgccgta gtggcgctgt tggaaggttg caatatcact gcattgccat ccacgttcgt
     5881 ggcattgatg ccggtgactt gcacgcggat ttcacctttg gaactggtct tgattgacgt
     5941 cacgtacttc gagacctggg cgactgtcga gctagattcg caaccccaca gtccagcggc
     6001 aggcagcgtg ctcgcggatt gcacggtttc cgtgatactg gtacggcatg ccgacgcggc
     6061 cagaatggct tccgacatct tggcgcgggc ggtgtagtcc tggtatgccg gcaacgcgac
     6121 cgcggccaga ataccgatga tcgcaacgac gatcatcaat tcgataaggg taaaaccctt
     6181 ttgtacggcc ttcatggtaa ctcctcaatg gttgatgaac aaaacactct aaaaattcgg
     6241 gtgttcgggg ctcaactttg caagccctat gccagacgcc ggaacattcg gcaccgaagc
     6301 tgcggcagga ctccatgaac ctgtaagtac attgattcac aaaggtataa aacggcagca
     6361 cagatagtct gggtcgaaag tctggcgaga taagctttgc cggaattagg tcgtgaatgg
     6421 attaggccat cgtctcaaac gacacgaagg agtgcaatcg gcattatctg acgatctttg
     6481 tcaccggtgc tgataccccg gaagcgccgc ctcatcgact tcgtcgatct gatccgggtc
     6541 aagaaattgt tcggcgtatc gcaaatagac tttcttgctg acgaatatat cgaacaggtc
     6601 cgggtcgata tgtccatcaa ccttgaatct accgaggatc tgcaacgatt ctgtaagcgt
     6661 cttgcctttt ttgtagggcc ggtctttcgc agtgagtgcc tcaaaaatat ccgcgattcc
     6721 cattacacgg gccggcacgg acatccgatc gcgcgttagc ccgcgagggt agcctttgcc
     6781 atccatgcgc tcgtgatgac caccggcata ttccgcgaca tttttcagat gcttcggcca
     6841 cggaagtgat tccagcatct tgatggtcac ttcaatatga tggttgatga tctggcgctc
     6901 ttcctgcgta agcgttccgg atcgaatggt tagattgccg acttcatcgg cggttaggaa
     6961 atccacctca cgcccttcaa cgtcacgcca cctatatttt ttgctgatta cccgcacacg
     7021 ctcctgatct tcttcgcgca ttgcttcgct tccaaagttg cagaaacgca ggaattgtcg
     7081 gtcttcctcg atttctcgca gctgcgcttt taaagctcga tcggcttttt cccattcccc
     7141 cgccgcatta ccggcagcat taagttttgc tttgaaacgc gttatatcgg catcgcgctt
     7201 aagtatttcg aaacgagtat cgattagcgt gatgcggtca aaaatggttt ggagcttggt
     7261 ttctttatcc accacatgaa ccggagtcgt tatcttgccg caatcatgca gaagccctgc
     7321 tattttgagt tcgtagcgat ccttgtcgct cattttgaaa tcggctagcg ggccgtggtc
     7381 gacttcatcg gcggcttgcg ccaacatcat tgtcagcatg ggaacgcgct gacaatggcc
     7441 gcccgtatag ggagacttat cgtcgattgc tgcattgatc agattgatga acgactcaaa
     7501 cagcccttcc agctggctga taagctgtcg attcgtgaga gcgacagccg cctgcgaggc
     7561 cagggattca gcgagccgct gatcggcctc cgaaaatgaa actgtttccc cggtatggcg
     7621 gtccttggcg ttgatcaact gaaggacacc tattatttcg tcctcatgat tcttcatcgg
     7681 gacggtcagg aaagatattg aacggtatcc ggtttttcca tcgaagttct tcgttccggt
     7741 gaaatcgaat cctttggcag cgtacgcgtc tgtaatattt acggtttcat ccctcaatac
     7801 ggaatacgcg accaccatgg aattattcgg ctgtccctca tcgtcgtaca ggcgcacggg
     7861 atagaagggc acctcgactc cagtggttcc tcccatcgcg atgtcgagcg aatcagtccg
     7921 tacaatctcg aagtggacca gctgtttctc ggcatccacg cggtaaagcg ttccaccgtc
     7981 ggcattggtt atcttttttg cggccagcaa aatggcctcc agcagccgat tggtatcttt
     8041 taccctggac agcgatatgc cgacttgatt gagctcttcc attctgcgga agagttcatc
     8101 ggaagtgggt ttagcgatca caacacggaa tccgaacggc tgactctcta acgccgtgca
     8161 ctacttgatg cagacagccc tgacttgcag catatcagtg atgccttgaa gaactttttc
     8221 aatgccatcc tgctttaacg tccgcatgcc ctctgcaagg gcggtggcga cgatctcggc
     8281 cacccgtgcg tgctcctgaa tgtgcttttt tacagtatcg gttcccacta gcaattcgtg
     8341 cagaccgacg cgtcctttgt acccggttcc gccacaggca tcgcagccca ccggagtaaa
     8401 aagggtgaaa tgtcccttgt cgtccgcgaa catcttcgcc cattcccggt agacggcctc
     8461 atacgcagct ttgggatcgc gcttccaatt ctccgtattg cgcaattcca tgctgtactc
     8521 ttccagtatg gctttgagtt cctcctgagt cgcgacgtgc ggtgttttgc attttgaaca
     8581 cagacgtttt gcgagacgct gtgccagaac tcccagcagg gcatccgcaa aattgaacgg
     8641 atccatgccc atatcgagca atcggatgat agattccggt gcactgttgg tatgcaatgt
     8701 agcaaataca aggtgaccgg ttaacgatgc ctcgataccg gtggccgtgg tttccttgtc
     8761 tcgcatttct ccaaccatga tgatgtccgg atcggcccgc agaaacgctt tcattgcgac
     8821 cgcgaaagtg agccccgcct tggggttcat ttgaacttgg cgtaagccct tttgtgtaat
     8881 ttcgacaggg tcttccgctg tccatatctt ggtctccggc gtattgagat acccgaggac
     8941 cgaatgtaac gtcgttgtct taccggaccc ggtcggtccg cacacaaaga acaaaccgta
     9001 cggcttgctc accgtttcct tgagaaggcc aacattgcgc tgtgatagtc cgagtttgtc
     9061 gataggaatg ggctccccgg cagccagaat ccgcattacg atatcttcga caccgcctgc
     9121 tgacggtatg gttgcaacgc gcaattcaat gtccaggggg ccgaacttct tgaacttgat
     9181 cttgccgtcc tgaggttttc tcttttccga aatatccaaa tcgcacatta ctttcaagcg
     9241 cgccgcaatc gcgttgcgat agcttgcagg cacggaaatg tagttgtgta gagttccatc
     9301 tttgcggaat cgtacttctg tcttgccctt gcccgggtaa ggctcgatgt gaatgtcaga
     9361 tgcgccctgg tggtaggcgt ctacgattat tttattaacg agtttcacca gttcgttgtc
     9421 ctgcgctagc gaagcgtcat ctgtagaagc gcctccttct tcgtcccctt cttcgtccat
     9481 ggtggacaga agctcaccaa tcgaagacga accaccggaa tctgcgccaa aaaactggtc
     9541 gagggtggct ccaaattccc ggtgcgtaca gactttatag acaactttgt gcttgggaaa
     9601 tatattgctg accaccttgg atgcacgcac cttctcgggg tccgtcgtaa gcaccatcaa
     9661 tccctctttg acatcctcga cgggaagcca gaggctgctc tcgacgtagt cacgtttgag
     9721 gttcttgagt aaatcaagag gtttcacgcg gtcttgcctg aacggttcat acgaaacctt
     9781 gaagaagtgg gatagagcct cccctagcgc ggcaggttta acctgaaact catctatcaa
     9841 gacgtcttca atatccagat tcttcctgcg tgccgagcgc gtggcaagtt ccatttcctc
     9901 tgcggaaaga acggcgtttg tgacgaggta atcgtatttt cccctcaaca tggcagtagg
     9961 gcggctgcgc tgacgcaacg ctatttccag ggtttcgcat aaatgcgttg ccccttcttc
    10021 cgcgatggtc gagaaggcat gcccgctttt ggaattgatc acttgaatta caccgaccaa
    10081 ttcgctcgca gcactatcca ctacaggggc aaccaacatt tgcttggtac gaaagcccgt
    10141 tcgcttgtcc acctccttca gaaatttgag attcgggtta tgcacgcgca attcattgtc
    10201 gtcgtaaaca tcccgaatgt ttatcaactt cttcgatagt gccacgaagc cagcaatact
    10261 ctgctcgttg acgggcagtt tcagatcttt gaatgacgag agtcccgtct taactttgga
    10321 aattatggaa gcccgatctt cggagagcac atagatggtc aggcgctctg cttcgaatag
    10381 cgagcagata tcctgggaca gttccagcat gatttcgtcg atgttgctgg ttgcatgaat
    10441 cttgtttgtt accgcctgga gcttcttctg gaattccagc ttgtggctca tctcggccaa
    10501 attttgtcca gcttgtggct tggactgaaa aactgtgctc atggcatgaa tccttctttc
    10561 atttgcggaa ggcaactctc gacccaacac tgatgatcaa acaatatcgc acgcgtatgg
    10621 cgcgcttcaa aacttgaaaa cctgcccgtt agaaagcatg cgcgggttgt agcgtccagc
    10681 gcactcctct acctcctgca tcgtcagagt cccggccccc ggtttgagat gcgtaataaa
    10741 tactctcgtc ggtcgtctta actttgccaa ctcttccgcc agcatgctgg gacagaggtg
    10801 tttggatgcg atcgctaatt ccttttccat attcgggaac gcggtttcga taaccaaata
    10861 gcgaaggttt gaaatcttgt ttacttcatc ccacaaagc
//
LOCUS       DATMZQ010000261         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_796700, whole genome shotgun sequence.
ACCESSION   DATMZQ010000261 DATMZQ010000000
VERSION     DATMZQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796700"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="VN496_08505"
     CDS             <1..1623
                     /locus_tag="VN496_08505"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HXF17032.1"
                     /translation="APSGMTFRVAAHSKASVMLDAGALTTAYPELTVTGGAGAHIRLT
                     YAEALIDEHGQKANRNETQGRDILGVYDEFIADGGRRRMFSPLIWRTWRYLQIDVATG
                     EQEVTIDRLAAHFTAYPFNARASFTSSDADLAEIWEVSWRTARLCAHETYMDTPYYER
                     LQYVGDTRIQALISYVMSGDDRLARQAIDAIDNSRIVDGITTSRYPSQLPQIIPTFSL
                     MWVGMVRDFSMYRDDDAFVRSHLAGTRTVLDWFMARQRGDGLIGKLTWWPFVDWTADF
                     KDGVPPDGREGGSAPITLQFIEALRDAADLETRLGDPARAAVYSARAEKAVDAIRKLC
                     WDDKTGLVADTPAKAHFSQHANALAIWLDVIPRDLHKAMMRKILDADPGSGGANPTKM
                     SDASYYFRFYLARALEHAGMADAYLSTLHPWRRMLQLGLTTWAETPEPTRSDSHAWSA
                     HPNYDLLRLVAGVRPAAPGFAEMVIEPHLGGLKTLSAAMPVPKGIVQVSYAVESDGVT
                     ATITLPQGLPGKLIWKARDFPLRAGEQTLQLP"
     gene            complement(1733..>2032)
                     /locus_tag="VN496_08510"
     CDS             complement(1733..>2032)
                     /locus_tag="VN496_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17033.1"
                     /translation="EPEEVFANAASGDARCAEFVALCHRALAAATATSIHMDGPGHFY
                     ITGTNAKFIDIGMLDRNVQEMVKMSPLQGSRFEVIPTSDELGIIGAAVNARAAVQ"
BASE COUNT          376 a          566 c          677 g          413 t
ORIGIN      
        1 gccccgtcag gaatgacctt tcgggtggca gcgcacagca aagcgtcggt tatgctggac
       61 gccggtgcgc tgaccacggc ttatcccgag ttgacggtta cgggcggcgc cggagcgcat
      121 atccggctga cgtatgcaga agcgttgatt gatgagcacg ggcagaaagc caatcggaac
      181 gaaacgcagg gacgagatat tctcggcgtt tacgacgagt tcattgccga cggtggacgg
      241 cgccgtatgt tcagcccgct gatctggcga acctggcgat acttgcagat cgatgttgcc
      301 actggtgagc aggaagtcac catcgatcgt ttggcagcgc actttacggc ttatccgttt
      361 aatgctcggg cgtcattcac ctccagcgat gcggacctgg ccgagatttg ggaggtcagc
      421 tggcggacgg cgcggctgtg cgcccatgag acctacatgg acacgccgta ttacgagcgg
      481 ctgcagtacg ttggcgacac gcgcatccag gcgctgatct cctacgtgat gtccggcgat
      541 gaccggctag cgcggcaggc tatcgatgcg attgataatt caaggatcgt cgacggcatc
      601 accacgagcc gctacccctc acagcttccg cagatcattc ctacgttttc gctgatgtgg
      661 gtgggcatgg tgcgagattt ttcgatgtat cgcgatgatg acgctttcgt gcgcagccac
      721 ttggcgggga cgcgcacggt gctcgactgg ttcatggcga ggcagcgcgg ggatgggctg
      781 attggcaagc tgacatggtg gccgttcgtg gattggaccg cggatttcaa ggatggcgtg
      841 ccgccggatg gcagggaagg cggatcggca ccgatcacct tgcaatttat tgaagcgctg
      901 cgcgatgcag cggacctgga aacgcgtctg ggcgatcccg ctcgagcagc ggtctactcc
      961 gcgcgcgcgg aaaaggcggt agacgcgatc cgaaaacttt gctgggacga caagaccggc
     1021 ctggtggcgg atacgccggc gaaagcgcat ttcagccagc acgccaatgc gctggcgatc
     1081 tggctggacg tgattccacg cgacctgcac aaggcgatga tgcgaaagat cctcgatgcc
     1141 gatccgggct ccggcggagc gaatcccacg aagatgtcgg atgccagcta ctactttcgg
     1201 ttttacctgg cgcgcgcgct ggaacatgcc gggatggccg acgcttatct ctccacgttg
     1261 catccgtggc ggcgcatgtt gcagctgggg ctaacgactt gggccgagac gcctgagccg
     1321 acgcgatcag attcgcacgc ctggagcgcg catccgaact acgatttgct gcgcctggtg
     1381 gctggtgttc ggcctgcggc gcccggattc gcagagatgg tcatcgagcc gcatctgggc
     1441 gggctgaaaa cactctcggc cgctatgccg gttccgaagg gcattgttca agtttcctat
     1501 gccgtcgagt ccgacggagt gaccgcaaca attacgctgc cgcagggatt gcctgggaaa
     1561 ttgatttgga aggcgcggga ttttccgctg cgtgcgggtg agcagacgtt gcagttgcct
     1621 tgagggtcgt ggattccgcg gtttcacaac cgcctagcta caaacgcgca caggtagctt
     1681 ctgaaaatgt gctgaggccc ttcgaggctc agggtaaaca ggagtgcctg tgctactgga
     1741 ccgcggcgcg ggcattgact gcggcgccga tgatgccgag ttcgtcgctc gtggggatga
     1801 cctcgaagcg gctgccttgc agcgggctca tcttcaccat ttcctgcacg ttgcgatcga
     1861 gcataccgat gtcgatgaat ttcgcattgg tgccggtgat gtagaagtgg ccgggtccgt
     1921 ccatgtgaat gctggtggcg gtggcagctg ccagcgcgcg atggcatagc gcaacgaatt
     1981 ccgcgcagcg cgcgtcgccc gaggcggcgt tggcgaacac ttcttccggc tc
//
LOCUS       DATMZQ010000262        23549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1249123, whole genome shotgun sequence.
ACCESSION   DATMZQ010000262 DATMZQ010000000
VERSION     DATMZQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23549
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249123"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..889)
                     /locus_tag="VN496_08515"
     CDS             complement(17..889)
                     /locus_tag="VN496_08515"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HXF17034.1"
                     /translation="MQKPFKTIALIGKYKSPEIAEPVLRLAKFLHERGIKVLLDPLTA
                     THIPQNSYAVLPLEEVGRAAELAIVIGGDGTMLNIARTFAPFDVALVGINQGRLGFLT
                     DLSMAGMFEAMAAMLDGKFVAESRMLLQAEVWSADNRVLDVVAFNDISVNKGAEGSLI
                     ELEVRIDGKFVYNLRSDGLIVTSPTGSTAYALSSGGPILHPSLSVIGLVPVCPHTLSN
                     RPIVVSSESEIRIMILRAADARAHFDSHSHYELKERDQIVVRRCAHSIRLLHPLGHNY
                     YHMLREKLHWSETL"
     gene            968..2005
                     /gene="hrcA"
                     /locus_tag="VN496_08520"
     CDS             968..2005
                     /gene="hrcA"
                     /locus_tag="VN496_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXF17035.1"
                     /translation="MATNDVFPSERAQRLLKMLIERYISDGQPVGSRALARFSGLELS
                     AASVRNVMADLEELGFISSPHTSAGRVPTPRGYRFFVDTLLKVRPLDQVEINQLEGQL
                     QPDNTQKLVAQASHLLSDLTRFAGVVMTPKRRTAAFRHIEFLSLAEKRILLIIVAPDG
                     DVQNRIIITDKAYKPSELSEAGNFLNQNFAGQTFEDIKRRLHEELRQLREDMTVLMTM
                     ALDAGNEAMGQHAEDYVLSGGHNLLHVQDLSSNMANLRKLFDMFEQKTSLLQLLDTSS
                     RAEGVQIFIGGESGLLPLDECSVVTAPYEVDGQVVGTVGVVGPTRMSYERVIPIVDIT
                     AKLLSSALSQH"
     gene            2089..3192
                     /gene="hemH"
                     /locus_tag="VN496_08525"
     CDS             2089..3192
                     /gene="hemH"
                     /locus_tag="VN496_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXF17036.1"
                     /translation="MRYLPEPQHTHGSIAKTGILLINLGTPKAPTPSAVREYLAEFLS
                     DPRVVEIARPLWWLILNGIVLRTRPRQSAAKYALIWTNEGSPLAVHTARQAMLLKGYL
                     GERIKTPHAVAWAMRYGQPSINEMLRELREQGCDRILVFPLYPQYAASTTASAFDAVF
                     DTMKQMRNMPAVRLVKHYHDHSAYIAALAKNINDYWMSNGRPDNLLMSFHGLPRFALE
                     RGDPYHCECQKTARLLGDELGLAPERCHVSFQSRFGRAKWLQPYTLQTVKELGKRKTG
                     RLDVVCPGFVADCLETLEEIAVENKAAYLKAGGGEFHYIPCLNEQHEWIQAMARISAE
                     NLHGWSLADWDAGAEMQRSVAGRERALAMGAQK"
     gene            complement(3214..3786)
                     /locus_tag="VN496_08530"
     CDS             complement(3214..3786)
                     /locus_tag="VN496_08530"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HXF17037.1"
                     /translation="MRKIVFVLALPMGILAATVTAEPLVRLHTSYYYIDGPSATVLLA
                     QLDKNGPLGVDGKRHPARTRWDIKWKLQSSQHGTSCTISEAAVIIGIAQTLPRWRGED
                     KGSTSLKQRWAKFTESLLRHEEVHKQHGMQAGKEIESALRRVEPASNCEDMEVRADKV
                     AQAIVSKYQKLDEAYDRETAYGLSEGGTLL"
     gene            3931..4494
                     /gene="grpE"
                     /locus_tag="VN496_08535"
     CDS             3931..4494
                     /gene="grpE"
                     /locus_tag="VN496_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXF17038.1"
                     /translation="MMQDQNKASSAEEANLADVNPERPAANDPAQEEPALETLLEKAE
                     NAAQEHRDAWLRAKADADNIRKRAQSDLVNAHKYALENFSAELIPVRDSLEAALASQN
                     ATPEALRSGVELTLKQLTAAFEKFSIREINPLGEKFDPHKHQSINMVESTAEPNTVVA
                     VLQKGYSLNDRILRPALVSIAKPRPEA"
     gene            4560..6491
                     /gene="dnaK"
                     /locus_tag="VN496_08540"
     CDS             4560..6491
                     /gene="dnaK"
                     /locus_tag="VN496_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXF17039.1"
                     /translation="MAKIIGIDLGTTNSCVAVMENGQPKVIENSEGARTTPSIVAYMD
                     DGEVLVGAPAKRQAVTNPKNTLFAVKRLIGRRFTEKEVQKDIDLMPYKIVQNSNGDAW
                     IEVRGKRIAPPEVSAQVLIKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKKEGDRKIAVYDLGGGTFDISIIEIAVVDKEHQFEV
                     LSTNGDTFLGGEDFDQRLMDYLADEFKKDQGIDLRNDVLALQRLKEAAEKAKIELSSS
                     QQTEVNLPYITADASGPKHLAIKITRAKFESLVEELIQKTMEPCRIAIKDAGVKLDDI
                     SDVILVGGMTRMPKVQEKVKEIFGKEPRKDVNPDEAVAIGAAIQAGVLQGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIQKNTTIPTKAQQVFSTADDNQTAVTIHVLQGEREMAGG
                     NKSLGQFNLADIPPAPRGLPQIEVTFDIDANGILHVSAKDKATGKENKIKIQANSGLK
                     EDEIKRMVKDAEEHADEDRRTRELVDARNQADTLIHSAKKSLAEHGDKADAGEKSKIE
                     AAIKDAEEAIKGSDKDAIEAKTKVLAEASHKLAEKMYAQQSGAGAGAGPQSESGSAKA
                     DEGNVVDAEFEEVKDKKSA"
     gene            6597..7715
                     /gene="dnaJ"
                     /locus_tag="VN496_08545"
     CDS             6597..7715
                     /gene="dnaJ"
                     /locus_tag="VN496_08545"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXF17040.1"
                     /translation="MAKRDYYEVLGVNRDVSEEDIKKAYRKLAMKHHPDRNPDNPKAE
                     ESFKEAKEAYEVLCDAAKRAAYDQYGHAGVDSHAGMGAGAAGFGGFADAFGDIFGDIF
                     GSGRSRSNVYRGADLRYNLEISLEEAARGTETRIRIPTLEQCENCKGSGAKPGSQPVT
                     CSTCNGQGQVRMTQGFFSIQQTCPKCQGSGKVIQSPCPTCSGAGRLKKQKTLSVKIPP
                     GVDEGDRIRLTGEGEAGVNSGPPGDLYVVMHIRPHEVFTREENDLHCEMPVSFSKAAL
                     GGEIEIPTLDGHAKIKIPAETQSGKVFRLRGKGIKGVRSQSFGDLLCHVVVETPVNLT
                     SRQKELLHELEQINERDSGRHNPRAKSWMDKVREFFEA"
     gene            complement(7799..8581)
                     /locus_tag="VN496_08550"
     CDS             complement(7799..8581)
                     /locus_tag="VN496_08550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXF17041.1"
                     /translation="MSVLSFPVGAIDYAAYGKLAASVVRVMVRVPESNRTLFGSGVVL
                     PDDRIATNCHVIPGTGNVLIMEGGEGTQGERGPSDPAADLCLLNPLGLNAARAQTGVA
                     QKLKVGDEVVAIGFGGGLGRSMSSGHVTALYPYHNGRVIQTDAAFRPGASGGGLFDSN
                     GNLVGITTFFKRNGAQSAYFAIPVEWVEGLPASLETAEATSSPFWMRPQNDQPRFLQV
                     ASYEADGKWTEMEAAARLWVQEEPDAVQPLEALERAMAGLGR"
     gene            complement(8783..10171)
                     /gene="cysS"
                     /locus_tag="VN496_08555"
     CDS             complement(8783..10171)
                     /gene="cysS"
                     /locus_tag="VN496_08555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXF17042.1"
                     /translation="MRELPGGSKLKIYSSLPRGKFAFEPISPGKVGIYVCGMTVYDFC
                     HVGHARVMVVFDVVKRWLAASGFQVTYVRNITDIDDKIILRAAQENVPVEVLTNRFIR
                     AMDDDAAALNILKPDIEPRATENVPRMVTLIQQLIDKGIAYRAPNGDVFYSVREFKGY
                     GKLSGKNLDDLRAGERIEVDSSKRDPLDFVLWKSAKPGEPSWDSPWGRGRPGWHIECS
                     AMSERYLGEHFDIHGGGQDLQFPHHENEIAQSEGAHGHIFVNYWMHNGFVRVDDEKMS
                     KSLGNFFTVREVLAKYDAEVVRFFIARAHYRSPLNYSDQHLDDAKAALARLYTALKAV
                     PAEPLVIDWDQPHAARFRDAMDDDFNTPDAIAVLFDLANEINRSRSRSAAVQLKSLAG
                     LLGLLQRDPEDFLQSTVGEFDPTAIERLIAERVAARKARNFAESDRIRDELLADGIVL
                     EDGPTGTTWRRH"
     gene            10293..11252
                     /locus_tag="VN496_08560"
     CDS             10293..11252
                     /locus_tag="VN496_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF17043.1"
                     /translation="MKPIALLKLVGALLLISQIQPGFAANSYEDASKLFKQGNYAAAL
                     DQIETVIAANPRDARARFLKGLILTEQNKPAEAIKVFTSLTEDYPELPEPYNNLAVLY
                     ASQGQYDKARKSLEMAIRTHPSYAIAHENLGDVYAKMASEAYDKALQLDRGNAAAQTK
                     LAMIKDLFSGSVVPGKGSPETTAEQAGPAAAPGQGTIPAKPAPQSKADSDAVLAAVNG
                     WAKAWSHKNADDYLGYYAPGFQVPGGEPRANWEATRRDRIVKPKQIEVTVGSPKISFD
                     AEGRAVVKFRQGYKSDTLNTSGAKTLTLVKNNDRWQIIQERMN"
     gene            11348..12481
                     /locus_tag="VN496_08565"
     CDS             11348..12481
                     /locus_tag="VN496_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXF17044.1"
                     /translation="MSITPPPVLRQLNSSEAMLVRALIDIRDSKFSSAYDHINSVLAV
                     NPNFRLAQLVKGDLLIARSRAINSIGNPSGGSVEQVAGLRDEARARLMRSQFEPSTDL
                     VPRYLVQLPQSERFALVLDSTKSTLFVYENSGKTPRYVADYYVTIGKNGLEKFREGDK
                     KTPVGVYNVVSQLPKERLTDFYGKGAYPLSYPNEWDRIRGRDGHGIWLHGTPRDTYSR
                     PPRASDGCIVLTNEDLETLSSKLQIGSTPIIIADTIEWAKPDEIDTLRADLAVAIESW
                     RRDWESRNTDSYLRHYSKQFSSNGLNWSQWAAQKHQINASKDWIKVQVTNMSLLLYPG
                     KEQMAVATFEQDYASSNLSNRMLKRQYWIREDNTWRILLEGAA"
     gene            12539..13108
                     /locus_tag="VN496_08570"
     CDS             12539..13108
                     /locus_tag="VN496_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXF17045.1"
                     /translation="MYKLFGIICAALLGTGAYAANPQVEIKTNLGAITVELRPDKAPK
                     TVENFLQYAKSGFYKNTVFHRVIPGFMVQGGGFSKTMEQKPTREPVGIESNNGLKNTI
                     GSIAMARTQNPNSATAQFFINVADNVFLNYTEPTVRGYGYTVFGTVVKGMDVVEKIAS
                     SPTGPGGPFPKDVPQQQVVIEDIVLLEGK"
     gene            13105..13602
                     /locus_tag="VN496_08575"
     CDS             13105..13602
                     /locus_tag="VN496_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXF17046.1"
                     /translation="MSNVKLQTSMGDIVVELDTALAPATADNFLQYVRAGFYDNTIFH
                     RVINGFMVQGGGFEAGMKQKPVRAPIQNEASNGLQNNAYTIAMARTSDPHSATAQFFF
                     NVVNNNFLNYRAASQEGYGYCVFGKVVEGRDVIDKIKAVPTGNRGFHQDVPTKDVVLI
                     TASVV"
     gene            13643..14368
                     /locus_tag="VN496_08580"
     CDS             13643..14368
                     /locus_tag="VN496_08580"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HXF17047.1"
                     /translation="MPHSLFISDLHLSPETSAATASLMHFLRDTAPHADSLYVLGDLF
                     EYWVGDEGMAQPHAKQIVQAFRALADQGVETYFMHGNRDFLVGERLAQASGFHILPDP
                     TVVDLYGTPTILMHGDTLCTGDHEYQNFRAMVRNPAWQKAFIAKPLDERVRMAQQVRG
                     RSEQSKSLKDMTIMDVSPIAVEQTFRAHQAARLIHGHTHRPARHVHIVDEHECERWVL
                     ADWYDRGSYLWCDASGCHAEPLP"
     gene            complement(14394..15575)
                     /locus_tag="VN496_08585"
     CDS             complement(14394..15575)
                     /locus_tag="VN496_08585"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HXF17048.1"
                     /translation="MRWDELELETLAVRAGIHRSHFNEHSEALYLTSSFVFKNAAEAA
                     ARFSGEDPGMVYSRYTNPTVAALQERLAALEGAECCIATASGMSAILACVMGLMKAGE
                     HIVASQSVFGATVQLFSNILKRFGVETTFVRATDLKAWQQAIQPNTKLLFLETPSNPL
                     TEVADIAAIADKAHERGALLVVDNCFCTPVLQRPLDMGADIVIHSATKYLDGQGRVLG
                     GAVLGRREVIFEGLTGFLRSGGPTLSAFNAWVILKGVETLGLRMQAHSQTALNLARWL
                     EEHPKVERVYYPGLPSHPQHQLAKMQQRSGGGIVSFDVKGGREAAWRVIDATQLISIT
                     ANLGDAKTTITHPASTTHGRLSAEVRRSSGLGEGLLRIAAGLEAATDIKTDLARGLAK
                     L"
     regulatory      complement(15645..15721)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(15777..17291)
                     /gene="purF"
                     /locus_tag="VN496_08590"
     CDS             complement(15777..17291)
                     /gene="purF"
                     /locus_tag="VN496_08590"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXF17049.1"
                     /translation="MCGILGVVAKSPVNQLLYDGLLVLQHRGQDAAGIVTADGSKFQM
                     HKGGGLVRDVFRTRDMRHLVGNIGIAHCRYPTAGSASSFAEAQPLYVNSPFGIVLAHN
                     GNLTNAEQLKRDLFLQDQRHVNTNSDSEVLLNVLAHELQEAATGYQLDPPAIFRAVAG
                     VHRRCRGAYAVVAMIAGYGLLAFRDPHGIRPLVVGESKTETGAEYLVASESVALDTLG
                     FDLVRDVEPGEAIFIDQDSNFYTRQCAANPQHNPCIFEFVYLARPDSVIDGISVYETR
                     MRMGKSLADKIKRDYSGLQIDVVIPIPDSSRPSAMELADNLGLPYREGFIKNRYIGRT
                     FIMPGHEVRRKSVRQKLNAIGMEFKDKNVLLVDDSIVRGTTSREIVQMARDAGARKVY
                     FASAAPPVRFPNVYGIDMPTSRELIATGRSNDEIAREIGADKLIYQDLDALVDDVRSV
                     NPEISQFETSCFNGVYVTGDVTAEYLAGVEAQRNDGQKGEENITQLDLNLATAE"
     gene            complement(17356..17847)
                     /locus_tag="VN496_08595"
     CDS             complement(17356..17847)
                     /locus_tag="VN496_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXF17050.1"
                     /translation="MTVFDLVAIVLVMVSVGFGIWRGMVREVLSLLSWILAFWLAKLF
                     AAVVAGWLPASWNHQGLRIGIGFVAVMLISVLVLSLLSSLIVHLVKAAGLTNSDRVMG
                     ALFGVLRGVLVLVLLVLLGGMTSEPREPYWRHALFSRPLERMALWAKPWLPEDVARRV
                     SFE"
     gene            complement(17935..18573)
                     /locus_tag="VN496_08600"
     CDS             complement(17935..18573)
                     /locus_tag="VN496_08600"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXF17051.1"
                     /translation="MAIRDEELQLKKRARRRLVGAIALVLLIVVFLPMVLDSEPKPLN
                     QDIAIDIPPIPKPESASLPQSVPVTPLPSQRPHIAGLPEGNPSTDIAPASDPKTVPLQ
                     NQTPRAETAPSAKVAQKHETKPVAASESKSEDSFVVQLGAFSNATNARALQKKLQESK
                     FKAYNELIKSPGGNQTRVRVGPYPTREAAEKARDRMKSLKLIIGNATVVRLD"
     gene            complement(18631..19887)
                     /gene="folC"
                     /locus_tag="VN496_08605"
     CDS             complement(18631..19887)
                     /gene="folC"
                     /locus_tag="VN496_08605"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HXF17052.1"
                     /translation="MPQSLSDWLAYLERIHPLAIEMGLERVSAVRDRLGLQPNFPIIT
                     VGGTNGKGSVCAMLDAILTSAGYRVGLYTSPHLVRYNERVRVAGRSVEDDALTRSFAR
                     VEQARTPDSLTYFEFGTLAAMDIFVQERVDVAVLEVGLGGRLDAVNVFDPACTVVTAV
                     DFDHMDYLGPDRESIGREKAGIFRAGVPAICGDEKPPATLTAHAANIGAPLVLNGRDF
                     GFKRGQGDWQYWSAQGKRSALPYPALRGTYQLANAASALVALEQMQHLLPVDMGAIRR
                     GLVEVDLPGRFQVLPGRPVVVLDVGHNPHAARGLSASLRELPSGGRLMAVFAMLKDKD
                     IAGVARPLKDQVQEWLVADLPGPRGADAARIEEALISIGINVPISRFESPAIAYQSAT
                     RSAGQDDKILVFGSFFTVGAVVQTFV"
     gene            complement(19927..20790)
                     /gene="accD"
                     /locus_tag="VN496_08610"
     CDS             complement(19927..20790)
                     /gene="accD"
                     /locus_tag="VN496_08610"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXF17053.1"
                     /translation="MSWLQKLLPPKIKRTAGASKKAVPEGLWSKCEICETVLYRADLD
                     KNLNVCPKCGHHNRLTARERVDLTLDPEGQFEIGAEVTPIDFLKFVDSKRYTERLEHA
                     RSDTGEEDALIVVQGAIKNVPLVLAVFEFRFLGGSMGSVVGERFVRGVQSCCEQNLPF
                     VCFTASGGARMQEGLMSLMQMSKTTAALTQLARARLPFISVLTDPTMGGVSASFAFLG
                     DVVIAEPNALIGFAGPRVIEQTVRQTLPEGFQRAEFLLEHGAIDMIVDRRQMRDKLVS
                     LITLLMRAPAA"
     gene            complement(20882..21682)
                     /gene="trpA"
                     /locus_tag="VN496_08615"
     CDS             complement(20882..21682)
                     /gene="trpA"
                     /locus_tag="VN496_08615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXF17054.1"
                     /translation="MSRIPETFRKLQAQKKKALIPFVTAGDPTPAMTVPIMRAMVNCG
                     ADILELGVPFSDPMADGPTIQRSSERALKHGVGLRDVLGFVREFRAADATTPVVLMGY
                     ANPIEAMGYESFATAAHQAGVDGVLVVDYPPEESRQLVEVLKKRGIDSIYLLSPTSQE
                     SRIRQVKDMASGYIYYVSLKGVTGAANIDLKEVAAKLPQIRSHIQLPIGVGFGIRDAQ
                     TARTVASVADAVVIGSRLVQEIESSAPDLAIRNLSALLGGIRQAMDQS"
     gene            complement(21763..22974)
                     /gene="trpB"
                     /locus_tag="VN496_08620"
     CDS             complement(21763..22974)
                     /gene="trpB"
                     /locus_tag="VN496_08620"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXF17055.1"
                     /translation="MYEALKAYKLPDAKGHFGPYGGVFVAETLMQALDELREQYESMR
                     QDSAFIAEFEYELKHYVGRPSPVYHAKRLSEHCGGAQIWLKREDLNHTGAHKINNTIG
                     QALLAKRMGKKRIIAETGAGQHGVASATVAARFGMECVVYMGSEDIKRQSMNVFRMKL
                     LGATVVPVESGSKTLKDALNEAMRDWVTNVENTFYIIGTVAGPHPYPMMVRDFNSVVG
                     RELRTQMPEAIGRQPDAILACVGGGSNAIGAFYPYIEDNDVRLIGVEAGGLGLASGKH
                     AATLCAGTPGVLHGNRTYLIQDENGQIIETHSISAGLDYPGVGPEHAWLKDSGRAEYV
                     SITDEEALAAFHALTRMEGIMPALESAHAVAHAMKLAPSLSKDKVLLVNLSGRGDKDM
                     NTVAERAGMQL"
     gene            complement(22967..>23549)
                     /locus_tag="VN496_08625"
     CDS             complement(22967..>23549)
                     /locus_tag="VN496_08625"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXF17056.1"
                     /translation="DALVAAKSGAHAIGLIFYTGSPRLVSRKVAREVVDALPAFVTSV
                     ALFVDARQSEIEGVIADVKPELLQFHGNEAPEFCASFGLPYIKAAKVRAGLDLLQYAR
                     RYRSAKGLLLDAAVDGSHGGTGSTFDWALIPDQLPLPVILAGGLQQDNVADAIRRVRP
                     WAVDVSSGVEAAKGIKDAAKIAAFIRGVRNADV"
BASE COUNT         5368 a         6787 c         6509 g         4885 t
ORIGIN      
        1 aagtcggcct ctgcgtttaa agcgtttcac tccagtgcag tttttcgcgc agcatatgat
       61 agtaattgtg acccaacggg tgcagcaacc ggatggaatg cgcgcagcgg cgcacgacta
      121 tctggtcacg ttccttcaat tcgtagtgcg agtggctgtc gaaatgtgcg cgcgcatcgg
      181 cggctctgag aatcatgatt cgaatctccg attcactcga cacgacgatt ggccggttag
      241 acagcgtgtg cggacacacc ggtaccagac cgatgacgct cagactgggg tgcagaatgg
      301 gaccccctga agacagcgca tatgcggtcg agccggttgg tgatgtcacg atcaaaccgt
      361 ccgagcgcag attgtagaca aacttgccat cgatgcgtac ttccagttcg atcaggctgc
      421 cttcagcacc cttgttgacc gaaatgtcat tgaaagccac cacatccaat acccgattat
      481 ccgcgctcca gacttcagcc tggagcaaca ttcgggattc cgccacaaac tttccgtcga
      541 gcatggctgc cattgcttcg aacatcccag ccatcgacag atcggtcaga aatcccaggc
      601 ggccctggtt gatgccaacc aaggccacat cgaacggcgc aaaggtgcgc gctatattga
      661 gcattgtgcc gtcgccgccg ataacgatgg ccagctcggc ggcccgcccg acctcttcga
      721 ggggcagtac ggcatagctg ttctggggga tgtgggttgc agtcagcgga tcgagcagca
      781 cctttatacc gcgctcatgc aggaacttgg cgaggcgcag cacgggctcg gcaatctcag
      841 ggctcttgta tttcccgatc agtgcgatgg tcttgaacgg cttttgcatg ttccgattaa
      901 agcacatttg cgacttcgct cgcgtcagag ccgacaggga gccactttgg cacggtagaa
      961 tgccgcaatg gcgacaaacg acgtatttcc gagcgaacgc gcgcagcgcc tgctgaagat
     1021 gctgatcgag cgttacatct ccgacgggca gccggtcggc tcgcgtgcgc tcgcgcgatt
     1081 ctccggcctg gaactgagtg cagccagtgt tcgcaacgtc atggcggatc ttgaagaact
     1141 cggtttcatc tcaagcccgc acacctctgc tggaagagtc cctacgccgc gcggttaccg
     1201 ctttttcgtg gatacgctgc ttaaggtccg gcccctggat caagtcgaga taaaccagct
     1261 ggaagggcag ttgcagcccg acaatacgca gaaactcgtc gcgcaggcgt cacatctgct
     1321 ttccgatctg acacgctttg cgggagtggt catgacgccg aagcgccgca cagcggcctt
     1381 tcgccacatc gagtttctga gcctggctga gaaacgaatt ctgctgatca tcgtcgcgcc
     1441 ggatggggac gttcaaaatc ggataatcat caccgacaag gcgtacaagc cttccgaatt
     1501 gagtgaagcg ggaaacttcc ttaaccagaa tttcgctggc cagacgtttg aagatatcaa
     1561 acgacgcctg catgaagagc tgcgtcagtt gcgcgaggac atgactgtgc tcatgacaat
     1621 ggcgctggat gcgggcaatg aagcaatggg tcagcatgcc gaagactatg ttctttccgg
     1681 cgggcataat ttgctgcacg tacaggattt gtcgtccaac atggcgaacc tgcggaaact
     1741 ttttgacatg ttcgagcaga aaaccagctt gctgcagctc ctcgacacca gcagccgcgc
     1801 agaaggagtg cagattttca ttggtggcga atctggactg ctgccgttgg atgaatgtag
     1861 cgtggtgacc gcgccgtacg aggtcgatgg acaggtagtc ggtactgtgg gcgtggtcgg
     1921 cccgacccgc atgtcatacg agcgcgtcat tccgattgtc gacatcaccg ccaaactatt
     1981 gtcgagcgcg ctctcccaac attgaatcga gctgcagcca ttggaacgga ccagcccttt
     2041 cgtccaccga tgtagcagac ctcatccttt ccggcccgga ggagcccgat gcgatatctt
     2101 cccgaaccac agcatacgca cggcagtatt gcgaaaactg gcattcttct gattaatctc
     2161 ggcactccaa aggcgccgac tccgagtgcg gtacgagaat atctggccga attcttgtcc
     2221 gacccgcgcg tcgtcgagat tgctcggccc ctgtggtggt tgatattaaa tggcatcgtg
     2281 ctgcgcaccc gtccgcgcca gtcggccgca aagtacgcgc tgatctggac caacgagggt
     2341 tcgccgctgg cggtgcatac cgcgcgccag gcgatgctgc tgaaaggcta cctgggtgag
     2401 cgaatcaaga cgccacatgc cgtcgcgtgg gcgatgcgct acggacagcc ctcgatcaac
     2461 gaaatgttgc gcgaactgcg cgaacaaggt tgtgaccgca tcctggtgtt tccgctttat
     2521 ccgcagtacg ccgccagcac gacagctagc gcgttcgatg ctgtattcga cacgatgaaa
     2581 cagatgcgca atatgcctgc ggtaaggctc gtgaaacact atcacgatca ttccgcctac
     2641 attgcggcac tggcgaaaaa catcaacgac tactggatga gtaatggacg tcccgacaac
     2701 ttgctgatga gctttcacgg gctcccgcgt ttcgccctgg agcgtggcga cccctatcac
     2761 tgcgaatgtc agaagacggc tcgcttgctg ggcgacgaac ttgggctggc gccggaacgc
     2821 tgccacgtct ccttccaatc gcggttcgga cgcgccaaat ggttgcagcc ctacacactg
     2881 cagacggtaa aagaactcgg caaacgcaag accgggcggc tcgatgtggt ctgccccgga
     2941 tttgttgcgg actgcctcga aacgctggaa gaaatcgccg tggagaacaa ggctgcttat
     3001 ctgaaagcag gcggcgggga atttcattac attccgtgcc tgaatgaaca gcatgagtgg
     3061 attcaggcga tggcgcgcat ttccgctgaa aacctgcacg gctggagcct ggcggactgg
     3121 gatgccggtg cagaaatgca gcgttccgtc gcgggacgcg aacgcgcttt agccatgggc
     3181 gcgcagaaat aagcaggaat aaatcgtgca agactacagg agggtgcctc cttcagaaag
     3241 tccgtacgca gtctcgcgat cgtatgcctc gtccagcttc tgatatttcg aaacaattgc
     3301 ctgcgcaacc ttatccgcgc gcacttccat atcctcgcaa ttacttgctg gctcgacccg
     3361 gcgcagcgcg gattcgatct ccttgcctgc ttgcatgccg tgctgtttgt gcacctcttc
     3421 atgacgtagc aaagactcag taaattttgc ccatcgctgc ttcagactgg ttgatccctt
     3481 gtcttctcca cgccaccgcg ggagcgtctg agcaataccg atgatcacgg ccgcttcgct
     3541 gatggtgcaa ctggttccgt gctgcgagga ctgtagtttc catttaatat cccagcgggt
     3601 gcgcgcggga tggcgtttgc catccacgcc aagcggaccg tttttgtcta gttgtgccaa
     3661 cagcacggta gcggacggcc catcgatgta gtaatagctg gtgtgcaagc gcaccaaagg
     3721 ctctgccgtg actgtcgcag ccagtatccc catcggcaag gccaatacaa acacaatttt
     3781 acgcatgcga aagcttcctc ctgttggccc ggaccaagcc cggttgggga ttcagaacgg
     3841 ccggtagtat agcgtttgat tatttccctc ctccggggtt gaaaccccgg cagttgcccc
     3901 aacgtctgtc cggaattcgt tcaggagatc atgatgcagg accaaaacaa ggcttcctcg
     3961 gccgaggaag ccaacctcgc ggacgtcaac ccggagcgcc cggcggcaaa cgacccggcc
     4021 caggaggaac ccgcccttga aacgttgctc gagaaggcgg aaaacgccgc tcaggagcat
     4081 cgggatgctt ggctgcgtgc caaggcggat gcagacaata ttcgcaaacg cgcgcaaagc
     4141 gatctggtga acgcgcataa atatgcgctg gaaaatttct ccgcggaact tattcccgtg
     4201 cgcgacagcc ttgaggcggc cctggcaagc cagaacgcga ccccggaagc gttgagaagc
     4261 ggcgtagaac ttacgctgaa acagctgacg gccgcgttcg agaaattttc aatccgcgag
     4321 atcaatcctt taggcgagaa attcgacccg cacaagcatc agagcatcaa catggttgag
     4381 tcgaccgctg aaccgaacac cgttgtggcc gttctccaaa aaggctacag cttgaacgat
     4441 cggatactgc gccccgcgct ggtaagcatt gcaaaaccaa ggcctgaagc ttgatttccg
     4501 gctaacagac cagatttgcg ggattaaagg catttttatt tttgagcaaa ggaaccaaca
     4561 tggcaaaaat catcggtatt gacttgggta ccacgaattc gtgcgtagcc gttatggaga
     4621 acggtcagcc aaaggtcata gagaactcgg aaggcgcgcg cacgacgcct tcgatcgtcg
     4681 cgtacatgga cgacggggaa gttttggtag gcgcgccggc caagcgccag gcagtcacca
     4741 atccgaagaa caccctgttc gccgtgaagc gcctcatcgg ccggcgtttt actgaaaagg
     4801 aagtgcagaa agacatcgat ctgatgcctt acaagatcgt gcagaacagc aatggcgacg
     4861 cgtggatcga ggtgcgtggc aagaggattg ccccgccgga ggtttccgcg caggtgctga
     4921 tcaagatgaa gaagaccgcc gaggattatc tcggcgagcc ggtcaccgag gcagtgatca
     4981 ccgtgcccgc ctacttcaac gattcccagc gccaagcgac caaagacgcc ggccgcatcg
     5041 ccggacttga agtcaaacgc attatcaatg agccgactgc cgcggcgctt gcctttggta
     5101 tggacaagaa ggaaggcgat cgcaagattg cggtttatga cctgggcggc gggactttcg
     5161 acatttcgat tatcgaaatc gcggtagtgg acaaagagca ccagttcgaa gttctctcca
     5221 ccaacggtga tacgttcctg ggcggcgagg atttcgacca acgtctgatg gactatctgg
     5281 cggacgagtt caagaaagat cagggcatcg atttgcgcaa cgacgtactg gcgctccagc
     5341 gtctgaagga agctgccgaa aaagccaaga tcgaattgtc ttccagccag cagactgaag
     5401 tgaatcttcc gtacatcacg gcggatgcat ccggaccgaa acatctggcc atcaagatca
     5461 cgcgcgccaa gttcgagagc ctggtcgagg aattgatcca gaaaacgatg gagccgtgcc
     5521 gcatcgccat caaggatgcg ggggttaagc tcgacgacat ctcggatgta atcctggttg
     5581 gcggtatgac gcgcatgccg aaggtccagg aaaaggtcaa ggaaatcttc ggcaaggagc
     5641 cgcgcaaaga cgtaaacccg gacgaagccg tggcaatcgg cgcagcgatt caggccggcg
     5701 tgctgcaagg cgaagtcaag gacgtgctgt tgctggatgt gacacccctt tccctcggta
     5761 tcgagacgct tggaggtgtg atgacgaaat tgatccagaa gaacacgacc attccgacca
     5821 aagcacagca ggtgttctcc acggcagatg ataaccagac ggccgtaacg atccacgtgc
     5881 tgcagggcga acgcgaaatg gctgggggaa acaaaagtct gggccaattt aacctggccg
     5941 acattccgcc cgcaccgcgc ggactgccgc agatcgaggt cactttcgac atcgacgcga
     6001 atggcattct gcatgtttca gccaaggaca aggcaaccgg caaggaaaac aagataaaga
     6061 tccaggcgaa ttccggtctg aaggaagacg agatcaagcg catggtgaag gatgcagaag
     6121 agcatgccga tgaagaccgc cggacgcgtg aactggttga cgcccggaat caggctgata
     6181 cgctcatcca ttcagccaag aagtcccttg ctgagcacgg cgacaaggcc gatgcagggg
     6241 aaaagtcgaa gatcgaagct gcaatcaagg acgcggaaga agccatcaag ggatccgaca
     6301 aggatgccat cgaggccaaa accaaagtcc tggcggaagc ttctcataag ctggccgaga
     6361 agatgtacgc gcaacaatcc ggcgctgggg caggcgcggg gccgcagtca gaaagcggca
     6421 gcgccaaagc cgatgaaggc aacgttgtcg atgcagaatt cgaggaagtg aaggacaaga
     6481 agtccgcctg accggttgtt caggcgctag tttttgaggg gtgagccggt cctccggcgc
     6541 acccctctgt gtcgtctgga aagctataac gacgttgcag cgagagaact gaaaaaatgg
     6601 cgaaacgcga ctactacgaa gtacttggcg tcaaccgcga tgtttccgaa gaggacatca
     6661 aaaaagcgta tcgcaaactg gcgatgaaac atcatccgga tcgcaatccg gataatccaa
     6721 aagcggaaga atcgttcaag gaagccaagg aagcttacga agtcctttgc gacgcagcca
     6781 aacgggccgc atacgaccag tacggccacg ctggcgtcga ttctcatgca ggcatgggag
     6841 ctggcgccgc tggttttggt ggttttgcag acgcgtttgg cgacattttt ggtgatattt
     6901 tcggaagtgg tcgcagccgc tcgaacgtct atcgcggagc ggatctgcgc tacaacctcg
     6961 aaatttcgct ggaggaggcg gcgagaggta ccgaaacccg aattcggatc ccaacgctag
     7021 agcagtgtga aaattgcaaa ggtagtggtg cgaaacccgg cagccagcct gttacctgct
     7081 ccacctgtaa cggccaggga caggtacgca tgacccaagg tttcttttcc atccagcaaa
     7141 cctgtccgaa atgccaaggt agcggcaagg tcatccaaag cccgtgccca acctgcagcg
     7201 gtgcgggccg cctgaagaag caaaaaacgc tttcggtcaa aattccaccc ggtgtagacg
     7261 aaggcgaccg catccggtta actggagaag gcgaggcggg cgtgaacagc ggtccaccgg
     7321 gcgatcttta tgtcgtgatg catatacgcc ctcacgaggt ctttacacgc gaagaaaacg
     7381 atttgcactg cgaaatgcca gtgagcttca gcaaggcagc actgggaggc gaaatcgaaa
     7441 ttccgacgtt ggatggtcat gccaaaatca agataccggc ggaaacgcaa tcagggaaag
     7501 tgttcagatt acggggcaaa ggcatcaaag gcgtacgtag ccagtccttc ggcgatctgc
     7561 tctgccatgt ggtggtagag acgcccgtca atttgacatc caggcaaaaa gaactactgc
     7621 acgagctgga gcagatcaac gagcgtgaca gcggtcgcca caatccgcgg gccaaaagct
     7681 ggatggacaa agtgcgcgaa ttttttgagg cgtagtcgca gcctcgctag gcagttgccc
     7741 tgatccaaca acggctgcgc attaacagcc gctcggcctg gggatcgtgg ccggcccgtt
     7801 atctccccaa tcctgccatc gctcgctcaa gcgcctctaa tggctgaacc gcgtctggct
     7861 cctcctgcac ccaaagcctc gccgccgctt ccatctccgt ccacttgccg tccgcttcat
     7921 aactggcgac ctgaagaaat ctaggctgat cgttttgcgg gcgcatccag aacgggcttg
     7981 aggtcgcttc agctgtctcc agagaagcag gcaggccttc gacccattca accggtatcg
     8041 caaaataagc tgactgcgct ccattgcgct tgaagaaagt ggtgatgccg accagattcc
     8101 cgttactgtc gaacaaccct ccgccagaag cgccaggacg aaacgcagcg tctgtctgaa
     8161 tcacgcgtcc gttgtgatag gggtaaagag ccgtcacatg cccggaggac atgctgcgtc
     8221 caagccctcc accgaacccg attgcgacga cctcatcgcc gactttcagc ttttgcgcta
     8281 ctcccgtctg cgcgcgcgca gcattaaggc ccaacggatt taggagacag aggtcggccg
     8341 cagggtcgct tggtccacgc tcgccctgcg tgccctcacc gccttccatg ataagcacat
     8401 tcccggtgcc tgggatgacg tggcaattag tggctatgcg atcatccggc aacacgaccc
     8461 cggagccgaa caatgttctg tttgattccg gtaccctgac catgactcta accacgctgg
     8521 ctgcgagctt gccgtaggcg gcatagtcaa tggcaccgac tggaaatgac aacacagaca
     8581 ttagaaccag caaagtgcag ttgcgcattt gaataccttc cccgcctttt ttggccaatg
     8641 tcccggtaat tgtcaaatgg accttttgct ttggaccgcc tcgaatcact ctattcggat
     8701 tgcgcaatac ggcaaccgca ctatcgatgc gagcaatcgg cgtaccaggg ctgcccatct
     8761 agcctggaag tctgggcggt agctagtggc gtcgccaggt tgtcccggtc ggaccgtctt
     8821 ccagcacaat gccgtccgcc aacagttcat cccgaatgcg atcggattcg gcaaaattgc
     8881 gtgcttttcg tgccgcaacc cgctcagcta taagccgttc aatggcggtt ggatcaaatt
     8941 cgcccaccgt gctctggagg aaatcttcgg ggtcgcgctg tagcagtcca agtaacccgg
     9001 ccaatgattt gagttgcacg gccgcggaac gagacctgct ccgattgatt tcgttggcca
     9061 gatcaaacag gaccgcaatt gcatcgggtg tgttgaagtc gtcgtccatg gcgtcgcgaa
     9121 atcgcgccgc atgcggctga tcccagtcaa tcaccagtgg ttcggcgggc actgccttta
     9181 gcgcggtata cagtcgcgct aaagctgctt tggcgtcgtc gaggtgctga tcggaatagt
     9241 tgagtgggct tcgatagtgg gcacgcgcga tgaagaaacg aaccacttcc gcatcgtatt
     9301 tcgccaaaac ttctctcacg gtaaaaaaat tgcctaacga cttggacatc ttctcatcgt
     9361 cgacgcgcac aaacccattg tgcatccagt aattgacgaa tatgtgcccg tgcgcacctt
     9421 cggactgcgc aatttcgttt tcgtgatgcg gaaactgaag gtcctgcccg ccgccatgga
     9481 tgtcgaaatg ctcaccgaga tagcgctcac tcatggctga gcactcaatg tgccatcctg
     9541 gccgtccacg gccccatgga gagtcccacg acggttcacc cggtttggcc gatttccaca
     9601 acacaaaatc gagcggatcc cgtttgctgc tgtcaacttc aatccgttca ccggctcgaa
     9661 gatcgtcaag atttttgccc gagagttttc cgtaaccttt gaactcacga acagaataaa
     9721 aaacatcccc gttgggcgcc cgataggcga tacccttgtc gatcagctgt tggatcaacg
     9781 ttaccattcg gggaacgttt tccgtggcgc gcggctcgat gtcaggcttc aggatattca
     9841 acgcggcagc gtcatcatcc atggcccgaa tgaaccggtt tgttagcact tcgactggta
     9901 cattctcctg cgccgcccgc aggatgattt tgtcgtcgat gtcggtgata ttccggacat
     9961 aggtcacctg gaaaccggaa gctgccagcc agcgcttgac gacatcgaat acaaccatga
    10021 cgcgcgcatg gcctacgtga caaaaatcgt ataccgtcat gccgcacaca tagatgccta
    10081 ccttgcccgg tgaaatgggt tcaaaggcaa attttccccg cggcaacgag ctgtagattt
    10141 tcaacttgga gccgccgggg agttcgcgca tgaaagcttg tcttaggtta gccgaggagc
    10201 gattttgaga ttgtggatac aaccgtcgtg ctggtagaat ctgctattga ttacaataac
    10261 ctaccgtttt ttattgaggt aaagtttaac acatgaaacc aatcgcactt ctgaagctgg
    10321 ttggtgcctt gctgcttata tcccagatac agcccggatt tgcggcgaac agctacgaag
    10381 atgcaagcaa gttgttcaag caaggcaact atgccgcagc cctggaccag atcgaaacgg
    10441 taattgcggc aaacccgcgc gatgctcgcg cccgcttttt gaaagggctg attctcaccg
    10501 agcaaaataa gccggcagaa gccatcaagg ttttcacctc ccttaccgag gattatccgg
    10561 agctgccgga gccctataac aatcttgccg tcctgtacgc ttcgcaaggc caatacgata
    10621 aggcgcgtaa gtcgcttgaa atggccattc gcacgcatcc gagctatgcc atcgcgcatg
    10681 aaaaccttgg agacgtttac gccaagatgg caagcgaggc atatgacaag gcgctccaac
    10741 tggatcgtgg caatgcggcc gcacagacca agcttgcgat gatcaaagat cttttttcag
    10801 gcagcgtggt gcccggcaag ggctcgccag agactaccgc ggagcaagcc gggccggctg
    10861 cggcgcccgg acaaggcacg ataccggcga aaccggctcc gcagtccaaa gcggattccg
    10921 atgcggttct tgccgccgtc aatggttggg ccaaagcctg gtcacacaag aatgcagatg
    10981 attacctggg gtattacgcg cccggctttc aagtgcctgg aggggagccc agggcaaatt
    11041 gggaagccac gcgccgtgat cgtatcgtca agcccaagca aattgaagtc accgtgggat
    11101 cacccaaaat ttcgttcgat gccgaaggcc gcgcggtggt caagtttcgc cagggataca
    11161 agtcggacac cttgaatact tccggcgcga agacattgac gctggtgaag aacaacgacc
    11221 gctggcagat catccaggag agaatgaact agtgggcgcg acacgctgct atcaggccgg
    11281 ctcccgccag acgcttctgc aagcgctgct tgctgttata gcagcgagcg cttcgtatgc
    11341 ctgggctatg agtatcaccc caccgccggt tctgcggcag ttaaactcct ccgaagcgat
    11401 gttggtgcga gcgcttatcg atatccggga cagcaaattt tcttccgcat acgaccacat
    11461 caacagtgta cttgcagtca atcccaattt ccgccttgca cagctggtga agggcgatct
    11521 tttgatcgcg cgctcgcgcg caataaactc tatcggcaac ccctccgggg gatccgtcga
    11581 acaggtcgcc ggcttgcgcg acgaggcacg ggcccgactc atgcggtcgc agtttgaacc
    11641 ctccacggac ctggtcccgc gttatttggt gcaattgccg caaagcgaga gatttgcgct
    11701 ggtgctcgat tccacaaaat cgacgctgtt cgtatatgag aattcgggga aaacgccccg
    11761 ctatgtcgcg gattactacg taacaatcgg caagaacggc ctggagaagt ttcgcgaagg
    11821 cgacaagaag acacccgtag gagtctataa cgttgttagc caactgccca aagaacgact
    11881 cactgatttc tacggcaagg gcgcgtatcc actcagctat ccaaatgaat gggaccggat
    11941 tcgcggccgg gatggccatg gcatttggct gcatggcacg cctcgcgata cctacagcag
    12001 accgccacgc gcgagcgacg gttgcatcgt gctgaccaat gaagatctgg agaccctgtc
    12061 atcaaagttg cagattggat ccacgccaat catcattgcc gataccattg aatgggcgaa
    12121 gccagacgaa atcgacacct tgcgtgccga tctcgcggtc gccatcgaaa gctggcgtcg
    12181 ggactgggag agccgcaata ccgattctta tctgaggcac tattcaaaac aattcagttc
    12241 gaatggtctg aactggtcgc agtgggcggc ccagaaacac caaatcaatg ccagcaaaga
    12301 ctggatcaaa gtccaggtta ccaacatgtc gctcttgctt tatccgggca aggaacagat
    12361 ggctgtcgcg accttcgagc aagattacgc aagcagcaat ctctcaaatc gcatgctcaa
    12421 gcgccagtat tggataaggg aagacaacac ctggcgcatt ttgcttgaag gcgcggcgta
    12481 gcaatgcagg ccgcgctacg ttgaagtcag aaaaataaac aaaacctcag gggaccgaat
    12541 gtacaaatta tttggcatta tttgtgctgc gctgttgggc actggtgcgt acgccgccaa
    12601 cccgcaggtc gagatcaaaa ccaatttggg cgcaattacg gttgaactgc gaccggacaa
    12661 ggctccgaaa accgtagaaa atttcttgca gtacgctaaa agcggctttt acaaaaacac
    12721 tgtgtttcat cgcgtaattc ccggcttcat ggtgcagggc ggcggtttca gcaaaaccat
    12781 ggaacaaaaa ccgacgcgcg aacccgtagg catcgagtct aataacggcc tcaaaaatac
    12841 tatcggctcg atcgccatgg cccgcacaca gaatccgaat tcagctacgg cgcaattctt
    12901 cattaacgtg gcggacaacg tattcctgaa ctacaccgaa ccgacggttc gagggtacgg
    12961 ctataccgtt ttcggaacag tggtcaaagg aatggacgta gtggaaaaaa tcgcaagttc
    13021 gcccacgggt ccgggcgggc catttccgaa agacgtccca cagcaacagg tggtgattga
    13081 agacatcgtc ctgttggagg gaaagtgagc aacgtcaaac tgcagacatc gatgggtgac
    13141 atcgttgtgg aactcgatac cgcgttggcg ccggcaaccg cggataactt tttgcagtac
    13201 gtgcgcgccg gtttctacga caacaccata ttccaccggg taatcaatgg attcatggtt
    13261 caaggcggcg ggttcgaagc cgggatgaag caaaagccgg ttcgcgcccc gatccagaac
    13321 gaagccagca acggactaca aaacaatgct tacaccattg ccatggcacg cacttcagat
    13381 ccgcattccg caacggcaca gttcttcttc aacgtcgtga ataacaactt cctgaattat
    13441 cgcgcggcca gtcaggaagg gtatggttac tgcgtgtttg gaaaagtggt ggaaggtcgg
    13501 gatgtaatcg acaagatcaa agctgtccca accggcaatc gaggatttca tcaggacgtg
    13561 ccgaccaagg acgtggtact cattacagcg tctgttgtat agctttgcgc catcgtagcg
    13621 cccagtccgt tcgtccgcag tgatgcctca tagtctgttt atttccgatc tgcatttatc
    13681 gccagagacc tctgctgcaa cggcaagcct gatgcatttt ttgcgcgaca ctgcgccgca
    13741 tgccgactca ctttacgtac ttggcgatct attcgaatac tgggtcgggg atgagggcat
    13801 ggcgcagcct cacgccaagc aaatcgtcca ggcgtttcgg gcgctcgcgg atcagggcgt
    13861 ggagacctac ttcatgcacg ggaacaggga ttttctggtc ggcgaacgcc tggctcaggc
    13921 gagcggcttt cacatacttc cagatccaac ggtggtggac ctttacggta cgcctaccat
    13981 actgatgcat ggcgacacgc tatgcaccgg cgatcacgaa tatcaaaatt tccgggccat
    14041 ggtgcgcaat ccagcgtggc agaaagcgtt tatcgccaag ccactggatg aacgtgtgcg
    14101 catggcccag caggtacggg ggcgtagtga gcagtcgaag agtctaaaag acatgaccat
    14161 catggatgta tcacccatag cggtggaaca gacgttccgt gctcaccagg ctgcgcggct
    14221 gatccatggg catacgcatc gacccgcgcg ccacgttcac atcgtcgacg agcatgagtg
    14281 cgaacgctgg gtattggcgg actggtatga ccgtgggtcc tatctatggt gtgacgccag
    14341 cggctgccac gcggaaccgt taccttaatt cgctctggag ggttccctag cgctcagagt
    14401 tttgccagcc cgcgcgcaag atcggtcttg atatccgttg ccgcttccag gccggctgct
    14461 atgcgcagca gaccctcccc cagcccggat gacctgcgta cctcagcaga cagacgaccg
    14521 tgcgtggtgc ttgccggatg ggtaatcgtc gtcttggcat cgccgaggtt tgccgtgatc
    14581 gaaatcagtt gcgtggcatc tatgactctc cacgctgcct cgcggccgcc cttgacatca
    14641 aaagagacga taccgcctcc gctgcgttgc tgcatcttcg cgagttgatg ctggggatgc
    14701 gaaggcaatc ccggatagta aacgcgctcg actttagggt gttcttccag ccagcgtgca
    14761 aggtttagcg ccgtttgcga atgcgcttgc atgcgcaatc cgagtgtttc gacacccttt
    14821 aggatcaccc acgcattaaa tgcggacagc gtcgggccgc cgcttcggag aaaaccggtc
    14881 aggccttcga agatcacctc gcgtcgtccc agtaccgctc cacccagcac ccggccctgt
    14941 ccatccaggt acttggttgc cgaatgaatg acgatgtccg cgcccatatc gagtggccgt
    15001 tgtagtactg gcgtacagaa gcagttgtcg acaaccagca atgcaccgcg ctcatgggcc
    15061 ttgtcggcga tcgcggcgat atcggcgact tctgtaagcg ggttggaagg cgtttccagg
    15121 aacagcagtt tggtattggg ctgaattgcc tgctgccatg ccttcaggtc ggtagcgcgc
    15181 acaaaggttg tttcaactcc aaagcgcttc aaaatgttgc tgaacaattg cacagttgca
    15241 ccgaaaacgc tttgcgacgc gacgatatgc tcgcctgctt tcatcaaacc cataacgcag
    15301 gccagtatcg ccgacatgcc ggaggccgtg gcgatgcagc actccgctcc ttccagtgcc
    15361 gccaggcgtt cctgcaaggc ggcgaccgtg gggttggtgt agcgcgaata caccatgcca
    15421 ggatcttcgc ccgaaaagcg cgcggctgcc tcggccgcat tcttgaacac gaagcttgac
    15481 gtcaggtaaa gcgcctcgga atgctcgttg aagtggctgc gatggatgcc cgcgcgcaca
    15541 gcgagtgttt caagttcgag ttcgtcccac ctcatcgatt caccctcatt tgccgcccgt
    15601 ccagtggtgc aaataaaaaa cccgcttgag ctttcgccaa gcgggtctgg aattcgacgc
    15661 gcgctttagc agtatttatt acgcgcccgc aagctgtcgt caaatcggcg cgaaacacga
    15721 aagctactcc cgggaccgcg cgctgtcaat cgtgcagtcc cggcatgcct attattttac
    15781 tcagcggtcg ccagattcag atccagctgc gtaatgtttt cctcgccttt ctggccatcg
    15841 ttacgctgcg cctcaacccc ggccaggtat tcggcggtaa catctccagt cacatagacc
    15901 ccgttgaagc atgaagtctc gaattgcgat atttcggggt tcacggatct cacatcgtcg
    15961 accagagcat ccaagtcctg gtaaattaat ttatcagctc caatttcacg tgcgatttca
    16021 tcattgcttc gtccggtcgc gatgagctca cgcgaagtcg gcatatcaat gccgtacacg
    16081 ttgggaaacc tgaccggtgg agcagccgaa gcaaaataaa ccttgcgcgc cccggcatct
    16141 cgcgccatct gtacaatttc ccggctggtc gtcccacgca cgatggaatc gtccacaagc
    16201 agcacattct tgtccttgaa ctccatgcct atggcattga gtttctgtct tacggatttt
    16261 cttctgacct cgtgcccggg catgataaaa gtgcgcccga tgtaacggtt cttgatgaag
    16321 ccttcccggt agggcaatcc gagattgtca gcgagttcca tcgccgacgg gcggctggaa
    16381 tcgggaatcg gaatgaccac atcgatctgc aatccgctgt agtctcgctt gatcttatcg
    16441 gccagactct tgcccatccg catccgtgtc tcatatacgg atatgccatc gataaccgag
    16501 tccggacgcg ccagatagac aaactcgaat atgcagggat tgtgctgcgg attcgcggca
    16561 cactggcgcg tgtagaaatt actgtcctga tcaatgaaaa tggcttcgcc gggctcgaca
    16621 tcccgcacca gatcaaaccc cagtgtgtcg agcgcaacgc tttctgaggc gaccaggtat
    16681 tcggccccgg tttcagtttt tgattcccct acgaccaagg ggcgaatgcc gtgaggatcg
    16741 cggaatgcca gcagaccgta accggcaatc attgcgacga cggcatatgc gccgcgacag
    16801 cgccgatgca cgccggccac cgcacggaaa atagcgggag gatcaagctg atacccggtg
    16861 gccgcctctt gcagctcgtg cgccagcacg tttaacaaca cttcagaatc ggaattggta
    16921 ttgacgtggc gctgatcctg aagaaacaaa tcgcgcttta attgttccgc attggtcaaa
    16981 tttccgttgt gcgcgagcac gatgccgaac ggggaattta catagagcgg ctgagcttcg
    17041 gcaaaggaac ttgcagaccc ggcagtggga tagcgacagt gcgcgatgcc gatattgccg
    17101 accagatgac gcatatcgcg cgtgcgaaat acatctcgca ccaatccacc gcctttatgc
    17161 atctggaact tgcttccatc cgcagtgacg attccagcag catcctggcc gcggtgctgc
    17221 agcaccagca gcccatcgta gagcagctgg ttcaccggtg acttggccac cacgccgagg
    17281 attccacaca tggctcagat ctccacgcct atttacaccg gcccggcagc cagtgattgg
    17341 gagacgcagt ataagtcatt caaagctcac ccgtcgggca acgtcctctg gaagccaggg
    17401 cttggcccat aaagccattc tctccagcgg cctggaaaaa agcgcatgac gccagtaggg
    17461 ttcgcgtggc tccgatgtca tgccgccgag caacaccaga agaaccagaa ccaatacgcc
    17521 acgcaggaca ccaaacaatg cccccatcac gcggtccgaa ttcgtaagcc cggccgcttt
    17581 gaccagatgc acgatcagtg aagaaagcaa gctcaacaca agtacgctga tcagcatgac
    17641 tgctacgaaa ccgatgccga ttcgcaagcc ttggtggttc cacgatgctg gtagccaccc
    17701 tgcgaccacc gctgcgaaca acttcgccaa ccaaaaagca agaatccagg acagcaaaga
    17761 caacacttcg cgcaccatgc cgcgccagat gccgaaaccc acggaaacca tcacgagtac
    17821 gattgcgaca aggtcgaata ctgtcatccg cagcgccgcg ttcctgcggg aaccgcatat
    17881 attggggtct gcgagcttgt tttcaatgac gcgcgcggca tctgcggatt ggcgtcaatc
    17941 gagccgaacg actgttgcat tgccaattat caacttcagc gatttcatgc gatcacgtgc
    18001 cttctccgcg gcctctctgg tcggatatgg tccgactcgc acgcgagttt gatttccgcc
    18061 tggacttttg atgagttcgt tataggcctt gaatttgctc tcttgcagtt tcttttgcag
    18121 ggcacgcgca ttcgtggcgt tggaaaacgc gccaagctgc acgacgaaac tgtcttcgga
    18181 tttgctttcg ctcgcagcga ccggtttggt ctcgtgcttt tgcgctacct ttgccgacgg
    18241 ggcggtctct gcccgtggcg tttggttttg cagcggaacg gtctttgggt cggaggcagg
    18301 cgcgatatcc gttgaaggat tgccttccgg taagcccgct atgtgcggtc tttgggacgg
    18361 tagtggcgta accggcaccg attgcgggag cgaagcactt tcgggctttg gtatgggcgg
    18421 gatgtcgatc gcgatgtcct gattcagcgg cttgggctcg ctgtccagca ccattggcag
    18481 aaagaccaca atgagcaaga cgagcgcaat cgccccgacc aaccggcggc gagcacgctt
    18541 tttgagctgc agttcttcat ctctgattgc cattgccatg aactgtgcgg gctggtggtt
    18601 tgtgggctac gggactgccg acgaacagcg ctacacgaaa gtctgcacta ccgctcctac
    18661 cgtgaaaaaa gagccaaaca ccaaaatttt atcatcttgt ccggcagatc tcgtggcgga
    18721 ttgatatgca atggcaggag actcaaaccg gctgatcgga acgttaatgc cgattgaaat
    18781 gagcgcttcc tcgatgcgcg cggcatctgc accgcgcgga ccaggcaagt cggccacgag
    18841 ccactcttgc acctggtctt tcagaggacg cgcaacaccg gcgatatctt tgtccttcag
    18901 cattgcgaac accgccatca agcgcccgcc gctcggcaat tcgcgcagac tcgccgataa
    18961 accgcgtgct gcatgtggat tatggcctac gtccagcacg acgacgggcc tccccggcag
    19021 cacctgaaag cgtccgggca aatcaacttc aaccagaccg cgtcgtatgg cgcccatgtc
    19081 tactggaagc aggtgctgca tttgctccag tgccaccagc gcgctagccg catttgccag
    19141 ttggtaggtt ccgcgcaacg ccgggtaagg caacgctgaa cgcttgccct gcgcactcca
    19201 atattgccag tcgccttgac cgcgcttgaa accgaaatcc cgaccattga gcacaagcgg
    19261 cgcgccgatg tttgcagcgt gagcggtcaa tgtggctggg ggcttttcgt cgccgcatat
    19321 tgccggtacg ccggcgcgaa aaataccggc cttttcccga ccgatacttt ctctgtcggg
    19381 tcccagataa tccatatggt cgaagtccac tgcagtcacg acagtgcacg cagggtcgaa
    19441 tacatttacg gcatcgagcc gtccgcccaa cccgacctcc agcacggcaa catcaacgcg
    19501 ttcttgcaca aaaatatcca ttgcggccag cgtgccgaat tcaaagtacg tcagcgagtc
    19561 cggagtgcgc gcctgttcca cgcgagcgaa tgagcgcgtc aaagcatcat cctccactga
    19621 acgcccggcg acccgcacgc gctcgttata gcgcaccaag tgcggtgagg tataaagccc
    19681 gacccgatat cctgcgctgg ttaaaatggc gtccagcatg gcgcagacag agccctttcc
    19741 attggttcca cctaccgtga tgatcggaaa attgggctgc aacccaagcc ggtctcggac
    19801 tgcggagacc cgctccagtc ccatttcaat agccaatgga tggatgcgtt caagataagc
    19861 aagccaatcg gaaagcgact gcggcaatgt tggatgtccg aagcaacgcg cgaaggcgat
    19921 gcgccgctac gcggcgggtg cgcgcatcag cagggtgatg aggctcacta gtttgtcgcg
    19981 catctgccgg cgatcgacga tcatgtcgat tgcgccatgt tcgagcagaa attctgctct
    20041 ctggaaacct tccggaagtg tctggcgaac ggtctgctcg atgaccctcg gtcccgcaaa
    20101 accaatcagt gcgtttggct ccgctatgac tacgtcgccg agaaacgcga aactcgcaga
    20161 tacgccaccc atggtcgggt cggtaagcac cgaaatgaat ggcaggcgcg cgcgggccag
    20221 ttgcgtcaga gccgcagtag tcttcgacat ctgcatcagc gacataagtc cttcctgcat
    20281 gcgcgctcca ccgctcgcgg tgaagcagac aaaaggcaga ttctgttcgc aacagctctg
    20341 cactccccgc acaaatcttt cgccaaccac cgaacccatt gaaccgccca gaaagcggaa
    20401 ttcgaacaca gccagcacca ggggcacgtt tttgatggct ccctggacca caatcaaagc
    20461 atcttcttca ccggtgtccg acctggcgtg ctcaaggcgt tcggtatagc gtttgctgtc
    20521 gacgaacttc aggaaatcta tcggagtgac ttccgcaccg atctcaaact ggccttcggg
    20581 gtccagtgtc aagtcgacgc gttctcgcgc ggtcaggcgg ttgtgatgtc cgcatttcgg
    20641 gcaaacattc agatttttat cgagatcagc acgataaagt accgtctcgc aaatttcgca
    20701 cttgctccac agtccttcgg gcacagcttt cttggacgcg cccgcagtac gcttgatttt
    20761 tggtggcagc agcttctgca gccaactcat cctgccgtcc tcttggtcgc gatattgcgc
    20821 gcgggccgat gcgatgtcgc ccggttgcca agtgccgaga acgtctttac cggttttggt
    20881 ttcaactctg gtccatcgcc tggcgtatgc cgcccagcaa cgctgaaaga ttacggatcg
    20941 caaggtcggg agcggacgat tcgatttcct gtaccagccg gctgccaata acgacagcat
    21001 ccgcgacgga tgcgacggtc ctggcggtct gcgcatctcg aatgccgaag ccgacgccaa
    21061 tgggcaactg gatgtgcgac cggatctgcg gcagcttagc ggccacctct ttcaggtcga
    21121 tgtttgccgc cccggtcacg cctttcaggg agacgtagta aatatatccg cttgccatgt
    21181 ccttgacctg ccgaatgcga ctttcctgcg acgtggggga caataaataa atcgaatcga
    21241 ttccacgctt cttcaatact tcgaccagtt gtcggctttc ctcaggagga tagtcaacga
    21301 ccagaacgcc gtccacgccg gcttggtgcg cggcggtagc gaatgactca tatcccatag
    21361 cttcaatggg gtttgcatat cccatcagta ctacgggtgt ggttgcatcc gcggcgcgga
    21421 attcgcgcac aaatcccagc acgtccctga gcccgacacc atgtttcagc gcgcgctcgc
    21481 tggagcgctg gatagtgggc ccatcagcca tcggatcgga aaaggggacc ccaagctcca
    21541 gaatgtctgc gccgcaattt accatcgccc gcatgatagg taccgtcatg gcgggcgttg
    21601 gatctcccgc agtgacgaag ggaatcagcg ctttcttctt ctgcgcttgc agctttctga
    21661 atgtttccgg gatacgcgac attgttgtga cgatagtgtc agccgcaaga tgaatcaaaa
    21721 cagcctggaa tctcactcat cacgcaattt tctcggtcgc gttcagagtt gcatgcccgc
    21781 gcgttcagca accgtgttca tgtctttgtc accgcgcccg gacaaattca ccagcaacac
    21841 tttatccttt gacagcgagg gtgccagctt catcgcatgg gcgaccgcgt gcgccgattc
    21901 gagtgccggc atgatgcctt ccatgcgcgt caacgcatgg aaagcagcaa gggcttcttc
    21961 atcggtaatg gaaacgtact ccgcgcgccc cgaatctttg agccaggcgt gctcaggacc
    22021 gacgccggga taatccaacc ccgcggatat cgaatgggtc tcgatgatct ggccgttttc
    22081 atcctggatc aaataagtgc gattgccgtg caggacaccc ggagtaccgg cacacaaagt
    22141 cgccgcgtgt ttacccgacg caagtcccag tccgccggcc tcaacgccga tcagccgcac
    22201 atcgttatct tcgatgtacg gataaaacgc accgatcgca ttcgaaccgc caccaacaca
    22261 ggccagtatg gcgtccggct ggcgtccgat ggcctccggc atctgggtac gcaattccct
    22321 ccccacgacg gagttgaagt ctcgcaccat catcggataa ggatgcgggc ccgccacggt
    22381 accgatgata tagaacgtgt tctctacgtt ggtcacccag tcacgcatgg cttcgttcag
    22441 cgcatccttg agtgtcttcg agccgctctc caccggcaca acagttgcgc caagcaactt
    22501 catgcggaat acattcatgg actgccgttt gatgtcttcc gatcccatgt ataccacgca
    22561 ctccatgcca aaccgtgcag ccacggtcgc cgacgcaacg ccatgctggc ctgcgccggt
    22621 ctctgcgatg atgcgcttct tgcccatgcg cttcgcgagc agcgcctgcc cgatggtgtt
    22681 attgattttg tgcgcgcctg tatggttcag gtcttcgcgc ttcagccaaa tctgcgcccc
    22741 gccgcagtgc tccgacaagc gcttggcgtg atataccggg ctggggcggc cgacgtagtg
    22801 tttcagctcg tattcgaact ccgcgataaa cgcggaatcc tgccgcatcg attcgtactg
    22861 ctcgcgcaat tcatcgagcg cttgcatcag cgtctcggcg acaaacacgc ctccgtaggg
    22921 tccgaagtgg cctttcgcgt cgggaagctt gtacgccttc aacgcttcat acatctgcat
    22981 ttctcactcc tcggatgaag gcggctatct tggccgcatc cttgatgccc tttgccgctt
    23041 caacgccgct gctgacgtcc accgcccaag gcctaacgcg acgtatggca tcagcaacat
    23101 tatcttgttg aaggccgcct gccaaaatta ccggcagagg aagttgatcc gggatcaggg
    23161 cccaatcaaa agtagatccc gtaccgccat ggctgccgtc cacggccgca tcgagcaaca
    23221 gccctttggc gctccggtaa cggcgcgcgt attgtaacaa atccaggcca gcccgaacct
    23281 tcgcggcctt gatgtaaggc aacccgaaac tcgcacagaa ctccggagct tcgtttccat
    23341 gaaactgcaa aagctccggc ttgacgtccg caataacgcc ctcaatctcg ctttgcctgg
    23401 catccacgaa tagcgcgacc gatgtcacga atgccggcag cgcgtccacg acttcgcgcg
    23461 cgactttgcg cgataccagt cgcggactgc ctgtataaaa gatgaggccg atggcatgcg
    23521 ccccgctttt cgccgcgacg agcgcgtcc
//
LOCUS       DATMZQ010000263         4010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1250065, whole genome shotgun sequence.
ACCESSION   DATMZQ010000263 DATMZQ010000000
VERSION     DATMZQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250065"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VN496_08630"
     CDS             <1..1477
                     /locus_tag="VN496_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HXF17057.1"
                     /translation="LGANWSSALEVAIRLINWSVTWQLLEGVHSPLFDHEEGSRLRRR
                     WLDSVYQHAEFVRGYFSLYSSANNHLIGEAAGLFIAALTWPHWHKARVWQATAQKILE
                     DEAQRQNAPDGVNREQAVAYHHFVLDLLLLCLLAGKQNKVWFSVTYETRIEAMLEYIA
                     SIMDVSGNLPQFGDSDDALVVRLTPSQSFCSYRSLLATGAIIFRSGEFKRKAGALDDK
                     TRWLLGSQADALFDAQSVERTRLPIRQHFPLGGYYILGSEFETADEIRLTVDAGPLGF
                     QSIAAHGHADALSFTLSVGGVEFLIDPGTYAYHTQGAWRRYFRGTAAHNTVRVDRQDQ
                     SEPGGNFMWVKKARAGCSQWASTVAEDLFEGWQNGYQRLKDPVMHWRKISLHKLQRRI
                     SIDDTLRMAGCHDIELLFHCSERCSVEAAPGGYLLKQDNKALLLKLPHVDSATSQVLV
                     GGTAPIFGWVSRRFDNKLPAPTILWQARFTGDVVLRTEIVC"
     gene            1674..2987
                     /locus_tag="VN496_08635"
     CDS             1674..2987
                     /locus_tag="VN496_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXF17058.1"
                     /translation="MKISVLGLGYVGTVSAGCLAQQGHEVLGVDPEPRKVDLINAGKS
                     PIIEKDIGGIIERQVAAGKLAATTDVIDAVQRSDLALICVGTPSKSNGDIELKYIRRV
                     CEEIGEAMRHHQGAPAVVIRSTMLPGTMREVVIPTLEACSGRRAGVEFGVCINPEFLR
                     EGTSVHDYFHPPKTVIGELNPASGNLVASLYSHLDAPMVRTDIETAEMVKYADNAWHA
                     LKVGFANEIGNLCKGLDVDGHRVMDIFCQDTKLNLSPYYLKPGFAFGGSCLPKDLRAL
                     LYKAKTLDLSLPILASVLPSNEQQVDRAVRSVIQKGTRRIGVLGFSFKAGTDDLRESP
                     VVELTERLIGKGFELKIYDSNVNIARIHGANRDYILNHIPHISRLMVASLEEVLEHAQ
                     TIIIGNAAAEFREVPKRLTENQTVIDLVRITNSRSVSGLYEGICW"
     gene            2988..>4010
                     /locus_tag="VN496_08640"
     CDS             2988..>4010
                     /locus_tag="VN496_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXF17059.1"
                     /translation="MGPPVMPPGDSRPRRVLILVENLPSPFDRRVWQEATTLRDAGYA
                     VSIICPTGKGFESKFERIDGIAIWRYDLPVEGEGAKGYAIEYSVALARSFMLCWKIFL
                     KDGFDVIQACNPPDLFFIIGRIFKLFGKKFVFDHHDINPELYEAKFGKRGVFYWLLLK
                     LERWTFRTADVSIATNESYRRIAMERGGMAPERVFVVRSGPSLERMKIGPPDDRLKRG
                     RSYLVGYVGVMGKQEGVDYLLRAIRYLVFNLHRTDIHFGLVGGGTSLGAMKGLAAELG
                     VADFVTFTGRVTDDEMLAMLNTADICVNPDIANEMNDKSTMNKIMEYMALGKPIVQFD
                     LAEGRYS"
BASE COUNT          849 a         1102 c         1185 g          874 t
ORIGIN      
        1 attgggcgca aattggtcca gtgccctgga agtggcaatc aggctgatca actggtccgt
       61 tacgtggcag ttgctcgagg gcgtccattc gccgttattc gatcatgaag agggctcccg
      121 gctcagacgc cgatggctcg attcggtcta tcagcatgcc gagttcgttc gcggttattt
      181 ttcgctttac tcctctgcca acaaccatct catcggcgag gctgcgggac tgttcatcgc
      241 ggccctcacg tggccgcatt ggcataaagc gcgagtgtgg caggcaaccg cgcaaaaaat
      301 tcttgaggac gaggcgcagc gccagaacgc ccccgatggc gtaaaccgtg agcaggcggt
      361 ggcttatcat cacttcgttc tggatctttt gctgctctgc ctgctggccg gcaagcagaa
      421 caaggtctgg ttctcggtaa cgtatgagac ccgcatcgag gctatgctgg agtacatcgc
      481 ctccatcatg gatgtaagcg gaaatcttcc ccagttcggc gactcagatg atgcgctggt
      541 ggtcagactt acgccaagcc agtcgttttg ctcctatcgc tcattgctgg ctaccggagc
      601 catcattttt cgcagtggcg aattcaagcg caaggcgggc gcactcgacg acaagacgcg
      661 atggctgctc ggcagccagg ccgacgcgtt attcgatgct caaagcgtag aacgaaccag
      721 gttgccgatc cggcagcatt ttccgctcgg cggctattac atactgggca gcgagttcga
      781 gacggccgat gaaatacgac tcaccgtcga tgcggggccg ctgggctttc agagcattgc
      841 cgcgcacggg catgcggatg cgctgtcatt tacgttgtct gtcggcggcg ttgagtttct
      901 gatcgacccc ggaacctatg cttatcacac gcagggtgcg tggcggcgtt attttcgcgg
      961 tacagcggcg cacaacaccg tgcgcgttga ccgccaggat cagtccgaac cgggtggcaa
     1021 tttcatgtgg gtgaaaaagg cgcgcgcggg ctgcagccaa tgggcgagta cggttgcgga
     1081 agacttgttc gagggctggc agaacggtta ccagcgtctc aaggatccgg tcatgcattg
     1141 gcgcaagatt tcgctgcaca aattgcagag gcggatcagt attgacgata cgctgcgcat
     1201 ggcaggctgt cacgatatcg aactgctgtt tcattgcagc gagcgctgca gtgtagaagc
     1261 cgctcccggc ggctatctac tcaagcagga taacaaagcg ctgctgctga agctgccgca
     1321 tgtcgacagc gcgacgtcgc aggtcctggt cggcggtacc gctcccatct tcggctgggt
     1381 ttccagacga ttcgataaca agctgccagc gccgacgatc ctgtggcagg cacggtttac
     1441 cggagacgtc gtcctgcgta cggaaatcgt ttgctagggg acactgcgcc aagccgtcct
     1501 cctctcaggc agcgctgtgt aattccccgt tgatccgacc ggactagtta ggccttccac
     1561 gggcgtcaat ccttaccttt actgttcggc tgcgtagtcc gccgaatgca cgggctgtat
     1621 atctgctgac gcgcaaattc ctttggtcgc attccccgag ttttaggaga accatgaaga
     1681 tcagcgtatt gggtctgggt tatgtcggca ctgtctcggc aggctgcctg gcacagcagg
     1741 gtcacgaagt attgggtgtc gatcccgaac cccgcaaagt cgatctgatc aatgccggga
     1801 aatcgcccat catcgaaaag gacattggcg gcatcattga gcgacaggtt gccgccggca
     1861 agcttgccgc aaccaccgat gtcatcgatg cggtacagcg cagcgacctc gctctgattt
     1921 gtgtcggcac gcccagcaaa agcaatggtg atatcgaact caagtacatc cgccgagttt
     1981 gcgaggaaat cggcgaagca atgcgtcacc atcagggcgc tccagcagtc gtgattcgca
     2041 gcaccatgct gcccgggaca atgcgtgagg tcgtcattcc gacactggag gcgtgttcgg
     2101 gccggcgcgc cggagtggaa ttcggtgtct gtattaatcc cgaattcttg cgcgaaggga
     2161 cttcggtcca cgactatttt catccgccga agaccgtcat cggagaactg aacccggcaa
     2221 gcggcaatct ggtggcaagt ctctatagcc atctcgatgc gccgatggtc cgcaccgata
     2281 tcgagaccgc cgagatggtc aaatatgcgg acaacgcgtg gcacgcgtta aaagtcgggt
     2341 tcgcgaacga gatcggcaat ctctgcaaag gactcgacgt cgacggccat cgcgtcatgg
     2401 atatcttctg tcaggacacc aagctcaatc tatcgcccta ctacctgaag cctggcttcg
     2461 ctttcggcgg ttcctgcctg cccaaggacc tgcgtgcgct tctctataag gcgaaaacgc
     2521 tggacctttc gcttccgata ctggcctccg tgctgccgag caacgagcaa caggtagatc
     2581 gggcagtgcg tagtgtcatc cagaaaggca cacggaggat cggtgtactg ggatttagct
     2641 ttaaagccgg cacagatgat ttgcgcgaaa gcccggtcgt ggaattgacc gaacgcctca
     2701 tcggcaaggg attcgaacta aagatttacg acagcaacgt caatatcgcc cgtatccacg
     2761 gcgcaaaccg cgactacatt ctcaaccata ttccgcacat ctcgcgcttg atggtggcga
     2821 gcctcgaaga ggtcctcgag cacgcgcaga ccattattat cggcaacgcc gcggccgagt
     2881 tccgtgaagt gccaaagcgt ctgactgaaa atcaaactgt catcgatcta gtgcgcatca
     2941 ccaattcccg gagtgtctcg ggtctgtacg aaggaatttg ctggtaaatg gggccgcccg
     3001 ttatgccgcc gggggatagc agacccagac gggttttgat cctggttgaa aatctgccca
     3061 gtccgttcga ccggcgcgta tggcaggaag ccactactct gcgagatgcg ggctatgccg
     3121 tctccatcat ttgtcccacc ggaaaaggat tcgaaagcaa gttcgagaga atcgacggca
     3181 tagcgatctg gcgctatgac ttgccggtcg aaggtgaagg cgcgaaggga tatgcgatcg
     3241 agtactcggt cgcgctcgcg cgcagcttca tgctgtgctg gaagattttt ctgaaagacg
     3301 gcttcgacgt catccaggcg tgcaacccgc ccgacctgtt ttttatcatc ggccgcattt
     3361 tcaagttgtt tggcaaaaag tttgttttcg atcatcacga catcaatccc gagctttatg
     3421 aggcgaaatt cggcaagcgg ggtgtgttct attggctatt gctgaaactg gaacgctgga
     3481 cgttccgcac tgccgacgta tcgattgcaa cgaacgagtc gtaccgtcgc attgccatgg
     3541 aacgcggcgg gatggcgccg gagcgcgtct tcgtcgtgag aagcgggccg agtctggagc
     3601 gcatgaagat tggtccgccg gacgataggc tgaagcgcgg ccggagttat ctggtgggct
     3661 atgtcggcgt aatgggaaag caggaaggcg tcgattacct gctgcgcgca atccggtacc
     3721 tcgtcttcaa tcttcatcgc actgacatcc atttcggatt ggtgggcggc ggtacttcgc
     3781 tgggcgcgat gaaaggcctt gccgcggaat tgggtgtggc ggatttcgta accttcacgg
     3841 gccgtgtgac cgacgacgaa atgcttgcga tgctcaatac cgccgatatc tgtgtcaatc
     3901 ccgacattgc caatgagatg aacgacaagt cgaccatgaa caagatcatg gagtacatgg
     3961 cgctcggcaa gccaatcgta cagttcgatc tcgcggaagg gcgctattcg
//
LOCUS       DATMZQ010000264        13809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1941800, whole genome shotgun sequence.
ACCESSION   DATMZQ010000264 DATMZQ010000000
VERSION     DATMZQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941800"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1630
                     /locus_tag="VN496_08645"
     CDS             143..1630
                     /locus_tag="VN496_08645"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXF17060.1"
                     /translation="MRAESSRQRASEIVGEAPISTSFVLPKPIVFVLAALAFLFAIDL
                     FAWAAYRDVKQASDELTRHVNTLERLQALPKIMLEMESNMRGYLLTGSNSSLNRYRAA
                     ADQLPGITQRALILVEEDPKQHRLIEAAAAAARDWLARYASPMVVKRNAGSANAESAA
                     ALGESMRQTAKYSKAERIRAQFEESIHLEREQVMAAQGKLTRKLQRIAMWIKARAFAL
                     LLALAALTLLLGRILAKLTGQMHSREIAEHQMRTSSANMRAISAASPLGMFMADGLGA
                     CVQANGSFERMTGLAEADILGNGWQSALHPEDQKRVVFAWNAFTTSGMPFSSVHRFVH
                     RNGKVVWTSMKCNNMTDGDRLIGFACSIEDISERREAEEALGRCDERLHLALEHSRLA
                     LFDWHIPSGEVSLSREWNIAMGYGPQELTTTTRRLNELIHPDDVEAFGEAIASTLKTG
                     KSMSRVELRVETRGGQWKRVYLSGRVAERDAAGRAVRLIGTIASD"
     gene            1837..3066
                     /locus_tag="VN496_08650"
     CDS             1837..3066
                     /locus_tag="VN496_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF17061.1"
                     /translation="MTALFSIATRGLLAALVMVTLAACQRSQSQPPAMPPPEVTVVIV
                     QPQDLQTTFEYVAQISGVREVEVRPRVSGILQKWNFTEGSKVVAGQSLFTIDAAPFRA
                     ALARAEADLAGAQATNDQAKRNAGRLKPLYEAKAVSQKEYDDATSAEQVSAANVKAAL
                     AAATEARLNISYTRVEAPISGITSRALQSEGSLVQAQQTLLTKIAQIDPVHVIFSFTE
                     AEHLKFTRAMADGTLRLPKDGRFDVKLKLADGNEYARPGKVDFTDVRVDPATGTIEAR
                     AVIGNPEGLLRPGQFARVQLSGGVRPNAIALPQRAILEGPGTKIVLTVNAQGIVEARP
                     IQVGDWSAAQWIITGGLKAGDRVIVDGVVKARPGSPVKIAQAPTAGDQTDRVPQGGQP
                     SAKSGANSEAPHEPKSK"
     gene            3082..6282
                     /locus_tag="VN496_08655"
     CDS             3082..6282
                     /locus_tag="VN496_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXF17062.1"
                     /translation="MFSRFFIDRPIFAAVLSIFLVLAGLASMRTLPIAQYPEIAPPVV
                     TVRATYPGASAEVLEQTVAAPLENQINGVENMLYMSSNSSSNGVVEINVTFDIGTNVD
                     QAALNVNNRVKQAEARLPQEVRRQGVSVEKNSTSFLQVLAFNSPDGTLDDLYVSNYVT
                     LNVLDTIKRVPGTTNVQIFGAKDYAMRIWLRPDRMTQLKLTTADLLRALNEQNAQFAA
                     GKVGQAPTGGPQELVYTITTKGRLSDPHEFDEIIVRANPDGSALRLKDVARVELASKD
                     YDFIGRVNGRQSALLGIFLQPGANALDVAEDVGKTMADISKRFPVGMEFQNVYDTTRF
                     VKVSIREVAKTLLEAMALVFLVVFLFLQNWRATLIPFAAVPVSLIGTFAGLFLLGYSI
                     NTLTLFGMVLAIGIVVDDAIVVLENVERIMHEEHKDAREAAIKAMTEVTGPVIAIVLT
                     LCAVFVPIAFLGGLTGELYRQFAVTIAIAVVLSGIIALTLTPSLCVLILKREHKTPLG
                     FFTWFNDWFRRVTGRYTSGAAWIIRRGAIGLVLFAGMVIVTIGLWKITPGSLVPDEDQ
                     GYYIGAVFLPDGATLQRTDKVVTEVLKAIQSNHANEFAIAFTGLDFLGGGYRNNAATI
                     FVTQRHWDERKGVGTHELIGDYFMKTAGIKEGLVLAFAPPPIFGLGNAGGFEFYVQNR
                     GEGGPQRLAAEMGKFLAAANKDPQLAGVQTLWRAGVPQLHVDVDREKAKALGVPINDI
                     FDTLSATLGSYYVNDFNKFGRTWQVLMSAEPGYRNKPDSVGELYVRSSMGTMIPLKTL
                     ANVSYTSGPDSMDRFNNLPAVKLLGQGAPGISSGQAISVVERIASDVLPADFSYDWGG
                     ASFQEKKSSGTSTLALGLATIMVFLILAAQYERWSLPLSVMMALPFGTFGALAAVWLR
                     GMTNDVYFQIGLVTLLGLAAKNAILIVEYALLKNQEGLSPSAAAVEAARLRFRPILMT
                     SLAFILGVTPLAFSSGAGAGARTAVGTGVMGGMLAATFLAIFFVPMFFKLITERHLTE
                     TRSTDQIRAEIEHHKLLAQQATENPHHVPPPRQETP"
     gene            6279..7721
                     /locus_tag="VN496_08660"
     CDS             6279..7721
                     /locus_tag="VN496_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXF17063.1"
                     /translation="MMRRSAALVAMVTTAVIAGGCMTVGPDYQRPKVETPEQWPDAAA
                     GAAVSATWWQSYGDPVLDRMVDEALQHNLDLRNAIARVDEARSALGISRADQYPGVSA
                     NAAASRNRISQESVISVPPGVDPKYNDYRATLNVSYEVDFWGKYRRATEAARAELLAS
                     KFNREAVRLALIADTAKGYFALRALDAQVAITRRTLSTRQASTALQRLRFEAGVASEY
                     DLRQVEAETAQAQALLPSIEQRLAAQETALSVLVGRSPRAIASQLMDRGSALEALTAP
                     PSIPAGLPSDILERRPDLRQAEQSLVAANARIGQAKAAYYPTISLTGFFGAESSMLSG
                     LFDAQARMWQFAGSATQTVFDAGRTRSQVGVAEARQQQALAQYQGAIQNAFKDALDAL
                     VAQRKAREAMEAEQKRIEALTSAMRLAQLRYDNGVASFLELLIAERALLDAQLNQVDA
                     QRAQLSASADLFKAMGGGWDGFVDTAKSDN"
     gene            8056..8574
                     /locus_tag="VN496_08665"
     CDS             8056..8574
                     /locus_tag="VN496_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HXF17064.1"
                     /translation="MKAFSNGFTLIELMIVVAIIGILASVALPAYQNYSARAKISEAL
                     LAGSVCRTTIAEIVQSEAILPTAGQWGCETQSAVTAASRYVAKIQTNSVGAIRVEIRG
                     VSAYANGQGIVFRPWADVARSGPVVGGDRVAMWDCGPDPSNTNDISTYVPASCRSSAT
                     QLGTVTGFASST"
     gene            complement(8604..9587)
                     /locus_tag="VN496_08670"
     CDS             complement(8604..9587)
                     /locus_tag="VN496_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HXF17065.1"
                     /translation="MLRAATQATAATTGTEPVCISAGEALARYGFADGHPLGSDRQAA
                     FLNNAEKNLLLANVQLCALRQATIEELGRFHTGDHIDRVQHAERDNLKFLDDGDTPVF
                     PGVFEAAATVVGSALEALSSVMNGTCRRTFQPIGGLHHARRNHSAGFCVFNDLGVVIE
                     TLRLQYGVRRIAYVDIDVHHGDGIFYSYEYDPDLIFADIHEDGQYLYPGTGNEQETGK
                     GAANGTKLNISLKPGAGDAQFMAAWPRVEAHVTKYAPDFVLFQCGADGLKGDPLAHLN
                     YSPAVHSTAARSLRRIADRFARGRWMAFGGGGYDRNNLAAAWTAVLQAMTD"
     gene            complement(9655..9960)
                     /locus_tag="VN496_08675"
     CDS             complement(9655..9960)
                     /locus_tag="VN496_08675"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXF17066.1"
                     /translation="MRKIYSAATLPDAHIVLGALAQAGIDGRVLNANAQSIMGEIPFH
                     HAWPEVWIMDAKDAERAREVIDQIERRKSDIGLAFCPYCHEENPADFHTCWNCAKEF"
     gene            complement(9971..11077)
                     /locus_tag="VN496_08680"
     CDS             complement(9971..11077)
                     /locus_tag="VN496_08680"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HXF17067.1"
                     /translation="MPLKTVSGDYGRPNKGRGATLNVEGRFEHLSREPFEDGWDVAPE
                     EGPARPETIVTAERVTTIISKNDSPDIPFTLSINPYRGCEHGCIYCYARPSHAYLNLS
                     PGIDFETRLFAKVNAADRLREELSKPGYSCSAITIGANTDPYQPAERDWKITRSILEV
                     ASDCNQPVSIITKNAMVERDLDILVSMAEKNLAHVYISVTTLEHELARRMEPRASAPS
                     RRIQAIRRVAEAGVPVSVMVAPVVPLLTDSETEHILEAARDAGALSAGYVLMRLPYEV
                     KELFKDWLMHHYPLKAEHVMSRVKQMRAGRENDPNFGSRMRGSGEFADLLRTRFEKAC
                     HRLGLNQQRRTLETGLFRHPRLDGQMDLFARSGG"
     gene            complement(11131..11976)
                     /locus_tag="VN496_08685"
     CDS             complement(11131..11976)
                     /locus_tag="VN496_08685"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF17068.1"
                     /translation="MIKDAAYYESMYNNRAAVPDFQTHFDSWAARSEQARRDLDVELD
                     VPYGDDRMETMDIFRPGSDAKALLMFIHGGYWRSLDKRHHSFVATPFLNAGAAVAAIN
                     YSLCPAVRVEDIVLQCLKAMAWLYRNAAEVGVPRNRIFVAGHSAGGHLAAMCVAAQWP
                     RYSRGLPEKVVQGGLSVSGIYDLVPIVRTPSINMDLHLTEADALKLSPAFMPAPSKIA
                     FNIAVGGLELGGFKEQHALLAGQWSGVIARDIPCPDDNHFTILNSFANPDTELGKAAL
                     GMMGL"
     gene            12399..13271
                     /locus_tag="VN496_08690"
     CDS             12399..13271
                     /locus_tag="VN496_08690"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXF17069.1"
                     /translation="MTEGFRYPELLVETSWLASHLNDAGVRVLDCTTHLPPLPDNSYY
                     TVKPGREDFLKQHIPGSAFVDMDRDVAETSAAYHFMLPSSEQFAQTMSNLGISRDTLV
                     VSYSAANHWWATRMWWMLKVFGHERAAVLNGGFQKWCKEGRPVESGGAKPRAAAHFEA
                     RPPRLEMIATQQDVLKSIGVADVCIINALRPEQHAGTGGVYYGRRGHITGSINLAAVA
                     TVDANNVYKGAEELRDMFAEVLSKPAVIAYCGGGIAATADAFVLAMLGHDNVRVYDAS
                     LSEWARNPALPMEL"
     gene            13287..>13809
                     /locus_tag="VN496_08695"
     CDS             13287..>13809
                     /locus_tag="VN496_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17070.1"
                     /translation="MLKHFKIDPNDFKGPVLVTGAGGCIGSWALTLLTEAGIPVSAMD
                     LTEDKRRPKLLMAESELAQVQWFTGDIADTRAVNNALEKSGAKAIIHLAALQVPFCKA
                     DPIAGAKVNVVGTINVFEAARKFGIKRVAYASSIAAHGVFDPNTMATLYGAYKFANEQ
                     TSRVYAQDWQISSV"
BASE COUNT         2976 a         3859 c         3929 g         3045 t
ORIGIN      
        1 tgtctgtcgt gaagtgtgcc gcgtgatcgc tgaaaaaagg caaggctcaa ctattgaagc
       61 ggggtttcgc tacccagcta gacaatatgc ggttctcact taaatccgca caagtccatg
      121 ggcgaataag gcatggaaca cgatgagggc tgaatcatcc cggcagcgtg ccagcgaaat
      181 tgttggcgaa gctccgatat cgacctcatt tgtgcttcca aaacctatcg tgtttgtctt
      241 ggcggccctt gcattccttt ttgccataga tttgttcgcc tgggctgcgt accgggatgt
      301 aaagcaagct tctgacgaac tgacacggca cgtgaatact ctagaacgct tgcaagcgtt
      361 gccaaagata atgctggaaa tggaatcgaa catgcgaggc tatcttctga ctgggtccaa
      421 tagctcgctt aaccgttatc gcgcggccgc cgatcaactc ccgggcatta cgcaaagggc
      481 acttatcctc gtggaagagg atccgaaaca acaccggctc attgaggcag ccgcggcagc
      541 ggcccgggac tggctggctc gttatgcgtc tccgatggtt gtcaaacgca acgccggtag
      601 cgcaaatgca gagagcgcgg cagcacttgg tgaatccatg cgtcagaccg cgaagtacag
      661 caaggcggaa cgcatccggg cacagtttga agagtcaatt caccttgagc gtgagcaggt
      721 catggcggct caaggcaaac tgactcgaaa attgcagcgc attgccatgt ggattaaagc
      781 gcgtgcgttc gccctgctcc tcgctctggc tgcgctaacg ctgttacttg gccgcattct
      841 cgcaaaattg accggccaaa tgcattcgcg tgagattgca gaacaccaga tgaggacatc
      901 atccgctaac atgcgcgcca taagcgccgc ttctccgctt ggaatgttta tggcggatgg
      961 tctcggcgca tgcgtgcagg cgaatggctc tttcgagcgc atgaccggtc ttgctgaagc
     1021 ggatattctg gggaatggct ggcagtccgc tttgcatccg gaagatcaaa aaagagtagt
     1081 tttcgcctgg aacgcgttca caacttccgg aatgccgttt tcatcagtcc atcgattcgt
     1141 ccaccgtaac ggcaaggttg tatggacttc gatgaaatgc aacaacatga cagacggtga
     1201 ccggttgatt ggcttcgcct gcagcataga agatatcagc gagcgccgcg aagccgaaga
     1261 ggcgttgggc agatgcgacg agcggcttca tctggccctg gagcattcgc ggcttgcgct
     1321 gttcgactgg cacattccct caggcgaggt ctctctgagt cgcgaatgga atatcgctat
     1381 gggctacgga ccgcaggagc taactacgac tacgcgccgg ctcaatgagc tgattcaccc
     1441 ggacgatgtg gaagcattcg gggaagcgat tgccagcacg ttaaagacgg gcaagtccat
     1501 gtcgcgagtt gaattgcgcg tggagacacg aggcggacag tggaaaagag tctacttgtc
     1561 cggcagggtt gcagaacgcg atgcggccgg cagagcggtc cgacttatcg gaacgatcgc
     1621 cagcgattaa tttcccgctc tggcatgagc cagacgtacc gttggagaac atcgtcgcga
     1681 agcgatctgg caaacatttt cgggcgctct agagaaaata tttttatatg caagtaaact
     1741 tcgcggctcg catccaatat cccccagtgt tctatcacgc tcgtccgcgt gacggatgct
     1801 acagatttgg cacgcaaaca tttgcaggag cttcaagtga ctgctttatt ctcgattgca
     1861 acacgcggcc ttttggctgc actggtaatg gttacgctcg ctgcgtgcca gagatcccag
     1921 tctcagcccc ccgccatgcc accgcccgaa gtgacggtcg tgatcgtgca gcctcaggac
     1981 ctgcaaacga cgttcgaata tgttgctcaa atttcaggcg tgcgggaagt tgaagtacgt
     2041 ccgcgcgtct ccggcatttt gcaaaaatgg aacttcacgg aaggcagcaa ggttgtcgcc
     2101 ggacaaagcc tgtttacgat agatgccgcc ccttttcgcg cagcgctggc aagagccgaa
     2161 gcggatctag ccggcgcaca agccactaat gatcaggcaa aacgaaacgc aggccgattg
     2221 aagccgctgt acgaagctaa ggcagtcagc caaaaagaat acgatgacgc cacttccgcg
     2281 gagcaggtta gcgccgccaa cgtcaaggct gcattggccg ccgcaaccga agcaaggctc
     2341 aacatttcct atacgcgagt ggaagcccca atctctggga ttaccagccg tgcgttgcag
     2401 tccgagggca gcctggtaca ggcacaacaa accttgctga ccaagattgc ccagatcgat
     2461 ccggtgcacg tgatattcag tttcacggaa gcggagcact tgaaattcac ccgcgccatg
     2521 gctgacggca cgctgaggct cccgaaggat gggaggttcg atgtcaagct gaagcttgcg
     2581 gatggaaacg aatacgcgcg gccgggtaaa gtcgatttca cggatgtacg cgtggacccg
     2641 gcaaccggca ccattgaggc acgcgcggtt atcggaaatc ctgagggatt attgcgtccg
     2701 ggccagtttg cccgcgtgca gctatctggc ggtgttcgcc cgaacgccat agcgcttccg
     2761 cagcgcgcca tcctggaggg gcccggaacg aagatagtgc tcactgtcaa cgcgcaaggc
     2821 atcgttgaag cgcgtcccat acaggtgggc gactggtctg ctgcccagtg gataatcact
     2881 ggtggcctga aggccggcga cagggtcatt gtcgacgggg tggtaaaagc ccggccgggt
     2941 tcgccggtga aaattgcgca agcgcctaca gcaggcgatc aaactgatcg cgtaccccag
     3001 ggtggccagc caagcgccaa gtccggcgcc aattccgaag cgccgcatga gcccaagtcc
     3061 aaataatcgg cggaattaat cttgttctcc cgtttcttca ttgaccgccc gattttcgct
     3121 gcggtgctgt cgatcttttt ggtcctggct ggcctcgctt ccatgcgcac gctgcctata
     3181 gcgcaatacc ccgaaatcgc gcctccggtg gttacggtcc gggcgaccta tcctggcgca
     3241 tcggccgaag tgttggagca aacggtagct gctccgctcg aaaaccagat caacggcgtg
     3301 gaaaacatgc tgtacatgag ttcgaactcg tcatccaacg gcgtagtcga gatcaacgtc
     3361 acttttgaca tcggaacaaa cgtcgaccag gctgcgttaa acgtcaacaa ccgcgtcaag
     3421 caagctgaag cgcgcctgcc acaggaagtc cgccgtcagg gcgtatccgt ggaaaaaaac
     3481 tccacctctt tcctgcaggt actggctttt aattcaccgg acggaacgct tgacgatctt
     3541 tacgtcagca attatgtgac gctgaacgta ctggacacga taaagcgggt gccgggcacg
     3601 accaacgtgc aaattttcgg cgccaaggat tatgcaatgc gcatttggtt gcggcctgac
     3661 cggatgacgc agctcaagct cactaccgct gatctattgc gcgcgctgaa cgaacagaat
     3721 gcgcagttcg ccgccggcaa agtaggccag gcgcccacgg gtgggccgca agaactggtt
     3781 tacacaatta ctaccaaggg aaggctgtcg gacccacacg agttcgatga aatcatcgtt
     3841 cgcgccaatc cggacggttc cgctctcaga ctgaaagacg tcgcgcgcgt ggaactcgcg
     3901 tcgaaggatt acgacttcat cggccgggtc aacggcaggc aatccgccct gttgggaatt
     3961 tttcttcagc cgggagccaa cgcgctggac gtagctgagg atgtcggaaa aacaatggcc
     4021 gacatttcca aacgttttcc ggtcggcatg gaatttcaga acgtttatga caccacgcgc
     4081 ttcgtcaagg tgtcgatacg cgaagtggcc aagacactgc tcgaggccat ggcgctcgtc
     4141 tttctcgtgg tattcctgtt cctgcagaac tggcgtgcta cgttgattcc gtttgccgcc
     4201 gtacccgtgt cactgattgg cacttttgcc ggcttgttcc tgctcggcta ttcaatcaac
     4261 acattgacct tgttcggcat ggttctggcc atcgggatcg tggtcgacga tgcaatcgta
     4321 gttctggaaa atgtcgagcg catcatgcac gaagaacaca aggatgcgcg tgaagctgcg
     4381 atcaaggcaa tgaccgaagt aactggccca gtcatcgcca tcgtgctgac gctctgcgcg
     4441 gtgttcgtcc cgattgcatt cctcggcggc ttgaccggcg agttgtaccg tcagttcgcc
     4501 gtgaccatcg ccattgcagt ggtgctgtcc ggaattatcg cgttgacgct gacccccagc
     4561 ctgtgcgtgt tgatcttgaa gcgcgaacac aagacaccgt tgggtttctt cacctggttc
     4621 aacgattggt tccggcgtgt caccggacgc tataccagtg gcgctgcctg gatcatccgc
     4681 cggggcgcaa tcggtctggt gctgtttgcc ggcatggtga tcgttaccat cgggctgtgg
     4741 aaaataacgc cgggtagcct ggtgccagat gaggaccagg gttactacat cggcgcggtg
     4801 ttcctgcctg acggcgccac cctgcaacgc actgacaagg tcgtaacgga agtactcaag
     4861 gcgatccaat ccaatcacgc caatgagttc gccattgcat tcaccgggct cgatttcctt
     4921 ggcggcggct accgcaacaa tgccgcgacg attttcgtga ctcaacgaca ttgggatgaa
     4981 cgaaaaggcg tcggaacgca tgaactgatt ggcgactatt tcatgaaaac cgccggaatc
     5041 aaggaaggtc ttgtgctggc gtttgctccg ccgcctatct ttggcctggg aaacgccggc
     5101 ggttttgagt tctacgtgca aaaccgcggc gaaggcgggc cacaacgact ggcggctgaa
     5161 atgggaaaat tccttgcggc ggccaacaag gatccccaac ttgcgggcgt acaaacgctc
     5221 tggcgggccg gcgtaccaca actgcacgtg gacgtggacc gtgaaaaggc caaggcgctc
     5281 ggcgtgccca taaacgacat ctttgacacg ctgtccgcca cgctcggttc ttattacgtc
     5341 aacgacttta ataagttcgg gcgcacctgg caggtgctga tgtcggcaga gccgggctat
     5401 cgcaacaagc cagactcggt aggcgaactt tatgttcgtt ctagcatggg cacgatgatt
     5461 ccgctcaaga cgctggcgaa tgtttcctat acgtcaggcc cggattcaat ggatcgcttc
     5521 aacaatctgc cggccgtcaa actgctgggc cagggggcac ccgggatctc ttccggccaa
     5581 gccatctccg tggtggaacg catcgccagc gatgtgctgc ctgcggattt tagctacgac
     5641 tgggggggag catcgttcca ggaaaaaaaa tcaagcggca cgtcgacgct tgcgttggga
     5701 ctcgccacta tcatggtgtt cctgattctc gcagcacaat acgagcgctg gtctctccct
     5761 ctgtcggtaa tgatggcctt acctttcggc acgttcggtg cgcttgcagc ggtttggttg
     5821 cggggcatga ccaacgacgt ctacttccag attggcctgg tgacgctact gggtctcgcg
     5881 gcaaaaaatg caatcctgat agtcgaatat gcgttgttga agaatcagga gggattgtct
     5941 ccttccgctg ccgccgttga agccgcacgt ttgcgcttcc gaccgatact gatgacgtcg
     6001 ctggcattca tacttggggt aacgccgctt gcattttcgt ctggggccgg cgctggcgcg
     6061 cgcactgcag tcggcacagg cgtcatgggg ggcatgctgg ctgcgacctt tttggcgatt
     6121 tttttcgtac ctatgttttt caaactcatt acggaacggc atctgaccga aacacgttca
     6181 accgatcaga tccgtgcgga aatagagcat cacaaattgc ttgcacagca agccacagaa
     6241 aatccgcatc atgtgcctcc gccaaggcaa gaaactccat gatgcgacgg tccgctgcgc
     6301 ttgttgcaat ggtcacgacc gcggttatcg cgggtggttg catgaccgtc gggcctgact
     6361 accagcggcc caaagtagaa acgcctgagc aatggcctga cgcggccgcc ggggcggcag
     6421 tgtccgcgac atggtggcag tcttatggtg atcccgttct tgaccggatg gttgacgagg
     6481 cgttgcagca caatttggat ctgcgtaatg ccatagcccg cgtcgatgag gcgcgctcag
     6541 ccctcggcat ctcgcgggcc gatcagtatc ccggggtatc ggcgaacgcc gccgcgtcgc
     6601 gcaaccgcat cagtcaggaa agcgtgatta gcgtgccgcc cggggtagat cccaaataca
     6661 atgactatcg ggcgacgctc aacgtttcgt acgaggtcga tttctggggc aaatatcgcc
     6721 gcgcgacgga ggcggcgcgg gcggaattgc tggcttcaaa gttcaatcgt gaggcagtgc
     6781 gacttgccct gatagcggat acggcgaaag gctactttgc gcttcgcgcc ctggacgccc
     6841 aggtagcaat cactcgacga acactttcta cgcgtcaagc ttccactgca ctgcagcgcc
     6901 tgcgtttcga agccggtgtc gcatccgagt atgacttgcg tcaagtagag gcagaaacgg
     6961 cacaggccca ggcgctgttg cctagcattg aacagcgcct ggcggctcag gaaaccgcgc
     7021 tgtcggtttt agtgggccga agtccgcgcg ctatcgcgtc gcaacttatg gatcggggtt
     7081 ccgccctgga agcgctaact gcaccgccct cgataccggc gggactgcct tctgacatcc
     7141 tggaacgccg ccccgatctc cgtcaggccg agcaaagctt ggttgcagcc aatgcgcgga
     7201 ttgggcaagc caaggcggct tactatccga ctatttcgct gactggattt ttcggtgctg
     7261 aaagttcaat gttgtccgga ttgttcgacg cgcaagcccg catgtggcaa ttcgccggct
     7321 cggccacgca aacggttttc gatgccggac gcacacgatc ccaggtcggc gtcgcagagg
     7381 cccgtcaaca gcaggcgctc gcgcaatatc aaggtgcaat ccagaatgcg ttcaaggatg
     7441 ccctcgacgc actcgtcgcc caacgaaagg cgcgcgaggc gatggaggca gagcaaaagc
     7501 gcattgaggc actcactagt gccatgcgac tggcccaatt gcgttacgac aatggcgtcg
     7561 cgagttttct ggaattgctc attgcggagc gcgcgctgct tgatgcccag ttaaaccagg
     7621 tcgacgcgca gcgcgctcag ctgtcggcga gtgccgatct gttcaaggca atggggggcg
     7681 gctgggatgg cttcgtcgat acggcgaagt ccgacaacta ggctgcatag cttcagcgtg
     7741 gaatccgcgc ctgatttagg tcccgaaacg ccaacccact tgtcataaca cgcgacgtga
     7801 ctttggctca tgcggctgtg tagctgccgt gcccgaattg ccttcgtgcc tgacgctttt
     7861 ggtctcgggt tggcaatttc cgcattgcac catgcggctt gggctttgta tgattagcct
     7921 acgaggcagg tgtacgtgct tgtttttaca tataacaatt tttgcatttc gcattgggag
     7981 ccgccaaatc tgagcgcggg cctgctttgt gcgttttaac cagagcaagc aaatctattg
     8041 ggctctggag caagcatgaa agcgttttcg aatggcttta cactcattga gctgatgatt
     8101 gtcgttgcaa tcatcggcat tcttgcgtcg gtcgcgttgc cggcgtatca aaactattct
     8161 gcgcgcgcca agatttcgga ggccttgctg gcaggaagcg tttgccggac gacgattgct
     8221 gaaatagtgc agtccgaagc tatcctgcct acggcggggc aatggggttg cgagacgcaa
     8281 agcgctgtga ccgctgcctc acgttatgtg gccaaaatac agaccaattc agttggtgca
     8341 atcagggtcg aaattcgtgg cgtgagtgcc tatgctaacg gacaaggtat cgtattccgg
     8401 ccttgggccg acgtcgcgcg ttccggaccg gtggtggggg gtgaccgagt cgccatgtgg
     8461 gattgcggtc cagacccaag caacaccaat gacatatcta cgtatgttcc ggcctcttgc
     8521 aggtcgtccg ctacacaatt aggcacggtt acgggatttg ccagcagtac ttagcgggaa
     8581 actcgtattc ttccggtgaa ggactaatcg gtcatcgctt gcagaacggc cgtccaggct
     8641 gctgccagat tgttccgatc gtacccacct ccgccaaagg ccatccatct tcccctcgca
     8701 aaacgatccg cgatcctcct gaggcttctt gccgccgtgc tatgcactgc aggcgaatag
     8761 ttcagatgtg cgagcggatc tcctttcaat ccatccgcac cgcattggaa tagcacgaaa
     8821 tccggcgcgt actttgtaac atgtgcttcg actcttggcc aagctgccat gaattgcgca
     8881 tcaccggctc ctggtttgag gcttatgttg agtttggtac cgtttgccgc gcctttgccg
     8941 gtctcttgct cgttcccggt gcctggatag aggtactggc catcttcatg tatgtccgca
     9001 aagatcaagt ccggatcgta ttcatacgag taaaaaatac cgtccccatg gtgcacatcg
     9061 atatccacgt acgcgatccg gcgaacgcca tattgcagac gtagtgtttc aattacgacg
     9121 ccaaggtcgt tgaacacgca gaaacccgcg gaatgatttc gcctggcgtg gtgcagtccg
     9181 ccgatcggct gaaaggtacg ccggcatgtc ccgttcatga cactagaaag tgcttcaagg
     9241 gccgacccga ccaccgttgc cgcggcctcg aacactccgg gaaataccgg cgtgtcccca
     9301 tcgtccagaa atttcaggtt gtcacgctcg gcgtgctgga ctctgtcgat gtggtcaccc
     9361 gtgtgaaaac gaccaagttc ttcgatcgtc gcctgacgca gcgcgcacaa ttggacattg
     9421 gccagcagca aatttttttc tgcattgttc aggaacgcgg cctgacgatc cgaaccaagt
     9481 ggatgtccat cagcgaagcc atatcgcgct agcgcttcgc ccgcggagat acaaaccggc
     9541 tccgtaccgg tggttgcggc tgtcgcttgc gttgctgctc ttagcatgac cggtaaaccg
     9601 aaaaatattg tatgcgccac cattcccaac acgtttcgcc gtacggggaa cagttcagaa
     9661 ttctttggcg cagttccaac aggtgtgaaa gtctgcgggg ttttcttcat ggcagtatgg
     9721 gcaaaaagcc aggccaatat cggatttccg ccgctcgatt tgatcgataa cctcgcgagc
     9781 ccgctctgca tcctttgcat ccataatcca tacctctggc caggcatggt gaaaggggat
     9841 ttcgcccatg atgctctgcg cgttcgcgtt caacactctg ccatcgatcc cggcttgcgc
     9901 caatgcgcca agcacgatat gtgcatcggg aagcgtggca gccgaataga tcttgcgcat
     9961 taggtcgccg ttatccgccc gatcgcgcaa acagatccat ttgtccatcc aaacgcggat
    10021 gccggaatag gccagtttcc agcgtgcgcc tttgctgatt taaaccgaga cggtgacagg
    10081 ccttttcgaa tcgcgtcctc agcaagtcgg caaactcgcc gctgcctcgc atgcgcgaac
    10141 cgaaattcgg gtcgttttca cggcctgctc gcatctgctt gacccggctc atgacatgct
    10201 cagccttgag gggatagtga tgcatgagcc agtctttgaa aagctctttc acttcgtacg
    10261 gcaggcgcat caatacataa ccggccgata gcgcgcccgc atccctggcg gcttccaaaa
    10321 tatgttcggt ttccgagtct gtaagtaacg gaacgaccgg cgcgaccata acgctgaccg
    10381 gcactcctgc ctcagcgaca cgccggatag cctgtatgcg gcgtgatggc gccgaagcac
    10441 gaggctccat cctgcgcgcc agctcatgtt cgagcgtggt gactgagatg tatacgtgtg
    10501 caaggttttt ttccgccatg gaaaccaaaa tatccagatc gcgctcgacc attgcgttct
    10561 tggtgataat gctcacgggc tgattacagt ccgatgccac ctccagaata gaccgcgtga
    10621 ttttccagtc tcgttccgcc ggctggtaag gatcggtgtt ggcacctatc gtgatggcgc
    10681 tacagctata tcctggtttg gaaagttcct cgcgcagtcg atccgcggca ttgaccttgg
    10741 caaatagtcg agtttcaaag tcaatccctg gggataaatt taagtaggcg tgcgaaggcc
    10801 ttgcataaca atagatgcat ccgtgctcgc agccgcggta cgggtttatg ctcagcgtga
    10861 acggaatgtc cggcgaatca ttcttcgaga taatcgtggt gactcgttcc gccgtgacaa
    10921 tggtttcagg acgggcgggc ccttcttccg gggccacatc ccaaccgtct tcaaatggct
    10981 cccgggacag gtgctcgaag cggccctcga catttaatgt ggcaccccgt cccttgtttg
    11041 gacgcccgta atccccggac actgttttaa gcggcatact gtttataata acagtattgc
    11101 ttccgaatcg cagccagttc ggggcaggat ttataacccc atcattccca gcgcggcttt
    11161 cccgagttca gtatccgggt tggcgaatga attcagtatc gtgaaatggt tatcgtcagg
    11221 gcacggaata tcacgcgcaa tgacgccgga ccactggcct gcaaggagcg catgttgctc
    11281 tttgaacccg cccaattcca ggccgcccac tgcgatattg aacgcgattt tgctcggagc
    11341 cggcatgaag gccgggctga gcttcagtgc gtcggcttcc gtcaggtgca aatccatgtt
    11401 gattgacggt gttcggacga ttggcaccag atcgtaaatg ccactgactg aaagtccccc
    11461 ctggacgact ttttcgggaa gtccccggga atagcgcggc cactgcgccg cgacgcacat
    11521 cgcggcgaga tggccgcccg ctgaatggcc ggccacgaag atacgattac gtggcacgcc
    11581 cacttcagcg gcgttccgat acaaccatgc catggccttg aggcattgca gcacgatatc
    11641 ctcaactctg accgccggac aaagtgagta atttatagct gcgactgcag cacccgcgtt
    11701 caggaagggg gtcgccacga acgaatggtg tctcttgtcc aaggatctcc agtagccacc
    11761 atgaatgaac atcagcaacg cttttgcgtc actgcccggc cggaatatgt ccatggtttc
    11821 catacgatcg tccccgtagg gcacgtcaag ctcgacatca agatctcttc gcgcctgctc
    11881 cgaccgcgcc gcccaactgt caaaatgcgt ttggaagtcc gggactgctg cccggttgtt
    11941 gtacattgat tcatagtaag cggcgtcttt tatcattggc actccatctt tttcaggttt
    12001 agtttttcat gacttgtgga agggaacgac ggagtaatgc ttcttgattg tggaatcatg
    12061 cagcaatcgg cctaggatgc aaattgccgg cgacccgctg cgcctgtcat ggaatttgat
    12121 atcgtttgcc aggccaatgt ttaatttcat ggtttcaatg gcttgttcgt gaatcggcag
    12181 cccggctgca tagctaacac tggcctcggg tctggacggg taaaaggcgg tgctcaggcc
    12241 agttcatggc cccatgggac gttgccggca gcttaaacct gctctgcagc cctggcggcc
    12301 gcaagaactc gcgcagtttt ccgcaaaaac aaaaaatggc ggaggctgcc tatctttttt
    12361 gatagccttc gcgtttcaaa aattaccggg acaagtccat gaccgagggc ttccgctacc
    12421 ccgaattgct cgttgagacc agttggctgg cctctcacct gaatgatgct ggcgtgcgcg
    12481 tgctcgactg caccacacac ctgccgccac ttcctgataa tagttattac acggtgaagc
    12541 ccggcaggga ggactttctc aagcagcata ttccgggctc ggcctttgtt gatatggatc
    12601 gtgatgttgc cgaaacttcc gccgcgtacc atttcatgct tccatcgagc gaacagtttg
    12661 cccaaacgat gtccaacttg ggcatcagcc gtgacacgct agttgttagt tattcagccg
    12721 ccaatcactg gtgggccact cgaatgtggt ggatgctcaa agtgttcggc cacgagcgcg
    12781 cggccgtgct gaacggcgga ttccaaaaat ggtgcaagga aggtcgaccg gttgaaagcg
    12841 gtggcgcgaa gccaagggcc gccgcacatt tcgaagcacg gccacctcgg ctggaaatga
    12901 tcgctaccca gcaagatgtc ctcaaatcga ttggggtagc cgatgtttgt atcattaacg
    12961 cattgcgccc ggagcaacac gccggtaccg gcggcgtata ttacggacgc cgcggccaca
    13021 ttacgggcag tatcaatctg gctgcagtgg ctacggtgga cgccaacaat gtgtacaaag
    13081 gtgcggagga gctgcgtgac atgtttgccg aggttctgtc caaacctgcc gtcattgcat
    13141 actgtggagg aggcatcgcc gcaaccgcgg atgcattcgt tttagccatg ctgggccacg
    13201 acaacgtcag ggtctacgat gcttcgctgt ccgaatgggc ccgaaatccg gccttgccaa
    13261 tggaactgta atgtgaggag acgcgcgtgc tgaagcactt caagatcgac cccaacgact
    13321 tcaagggtcc ggtactggtg accggggcgg gcgggtgcat cggcagttgg gcgttgaccc
    13381 tgctcacaga ggccggcatc ccggtgtctg ccatggatct gaccgaggac aaacggcgac
    13441 cgaaattatt gatggctgaa tccgaacttg ctcaagtgca gtggttcact ggcgatattg
    13501 ccgacacccg ggcggtcaac aatgcgctgg aaaagagcgg agccaaagcc atcatccacc
    13561 tggctgcctt gcaggttcca ttctgcaagg ccgatccaat agctggagcc aaagtgaacg
    13621 tggtcggcac catcaacgtc tttgaggcag cgagaaagtt tggtattaag cgcgtcgcgt
    13681 atgcaagttc aatcgccgcg catggtgttt ttgacccgaa cacgatggcg acgctatacg
    13741 gcgcctacaa gttcgcaaac gagcaaactt ctcgcgtata cgcacaagac tggcaaattt
    13801 ccagtgtcg
//
LOCUS       DATMZQ010000265         3119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_213869, whole genome shotgun sequence.
ACCESSION   DATMZQ010000265 DATMZQ010000000
VERSION     DATMZQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213869"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VN496_08700"
     CDS             <1..251
                     /locus_tag="VN496_08700"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HXF17071.1"
                     /translation="FPEVQLTRRPVKPYLSATRLTRFDCKAGRRANVESTLYRGRMAS
                     GDITDVYRTPDAELQFEWVNPDAPGGESMRRVCAASAG"
     gene            327..953
                     /gene="ccmA"
                     /locus_tag="VN496_08705"
     CDS             327..953
                     /gene="ccmA"
                     /locus_tag="VN496_08705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HXF17072.1"
                     /translation="MLEARKIECIRGDRRLFHGLSFRLEAKQALRISGDNGSGKTSLL
                     RMVAGLSPTQDGEICWNQARITLLAEDYRRELLFIGHANGLKEDLSALENLQHALVLA
                     GRHVEFGALRAALADQGLAAIAQLPVRVLSQGQKRRVALARLMFCADKTLWILDEPFG
                     ALDTIAVAYIEKLLLTHLQRGGMLLYTTHQEVALPGAQSLSVELGHAR"
     gene            950..1630
                     /gene="ccmB"
                     /locus_tag="VN496_08710"
     CDS             950..1630
                     /gene="ccmB"
                     /locus_tag="VN496_08710"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXF17073.1"
                     /translation="MSQSAVTACRAVIARDLLVAARRRSDVLTTFFFFVIVVSLFPLG
                     IGPQPDTLREIAPGVVWVAALLAALLSLARMFDADYADGTLEQVMLAPQPPVLMVLSK
                     ISAHWLTTGLPLVVIAPLLGLQFDLPGRARATLLLSLLIATPALSLIGAVGAALTLGV
                     RGAGGLSALLVLPLYVPVLVFGAGAVTASAAGLNEAAHLSLLAAMALVALVFAPWATA
                     AALRIALE"
     gene            1639..2367
                     /locus_tag="VN496_08715"
     CDS             1639..2367
                     /locus_tag="VN496_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HXF17074.1"
                     /translation="MSWFRYSSPQSFYPLAGKMVPWFFTLAALLGLAGLYIAFFVAPT
                     DFQQGDAYRIIFIHVPAAWMSMLIYAVMAFWAGIGLVFNTRLSSMMAHALAPTGALMT
                     LIALWTGSFWGRPTWGAWWVWDARLTSELILLFLYLGYLALVAAIDDPRRADKASAVL
                     ALVGAINVPIIYFSVQWWNTLHQGSTISMTRAPSMASIMLIGMLVMVFAFWAYSIAAA
                     LMRVRCEILEREHHSAWARKLEDI"
     gene            2369..2542
                     /gene="ccmD"
                     /locus_tag="VN496_08720"
     CDS             2369..2542
                     /gene="ccmD"
                     /locus_tag="VN496_08720"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HXF17075.1"
                     /translation="MQWSSAGEFLAMGGYGLYVWGAYAVTAVIMVAEVIALARRARTL
                     RAKSSRSADAGVD"
     gene            2552..3013
                     /gene="ccmE"
                     /locus_tag="VN496_08725"
     CDS             2552..3013
                     /gene="ccmE"
                     /locus_tag="VN496_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXF17076.1"
                     /translation="MKPRHKRMALILAGLAILGLAAALVLNAFRSNLVFFFTPTQVAA
                     HEAPQGRNFRIGGLVEKGSVKRQPDGVTVRFIVTDTSKSVPVTFTGILPDLFKEGKGV
                     VAQGKLGADGVFAASEVLAKHDENYMPPEAADAVERANKAMKESKKTLVKP"
BASE COUNT          581 a          836 c         1008 g          694 t
ORIGIN      
        1 attttccaga ggtgcaactg actcgcaggc cggtgaaacc ttatctttcg gcgaccaggc
       61 tgacacgttt cgactgcaag gccggccgcc gtgccaatgt ggaatccacg ctctatcgtg
      121 gccgcatggc aagtggcgac atcaccgatg tctatcgcac gccggatgcc gaacttcaat
      181 tcgagtgggt gaatccggat gccccgggcg gcgagagcat gcgtcgcgtg tgtgctgcat
      241 cggcaggctg agcgaggcta tgttgggtga tcgcttgcct gttaaatcgc cttataatgc
      301 cccgcccacg atagtcccgg atgccgatgc ttgaggcacg aaagattgaa tgtatccgcg
      361 gcgaccggcg actattccat ggattgtcat tccgattgga agcgaagcag gcgctgcgta
      421 tcagtggaga caatggcagc ggcaaaacca gcctgctgcg aatggttgcc ggcctgtctc
      481 ccacgcaaga cggtgaaata tgctggaacc aggcacgcat aacgctgctg gccgaggact
      541 accgtcgtga gctgctgttc atcggacacg ccaatggctt gaaggaagac ctgagcgcgt
      601 tggaaaacct gcagcacgcg cttgtgctcg ccggccggca tgtggaattt ggcgcattgc
      661 gcgccgcatt ggcggaccag ggtcttgcgg ccattgcgca gcttccggtc cgagtattgt
      721 cgcaaggaca gaaacgccgg gtcgcgctgg cgcgcctgat gttctgcgca gacaaaacat
      781 tgtggatact ggacgagccg tttggtgcgc tggacaccat tgccgtggcg tacatcgaaa
      841 agctgctgct aacgcacctg cagcgtggcg gcatgttgct gtacacgacg catcaggaag
      901 tggcattgcc tggcgcgcag tcgctgtcgg ttgaactggg acacgcacgt tgagccagag
      961 cgcagtaact gcctgccgcg ccgtcatagc gcgcgatctg ctggttgccg cgcggcgccg
     1021 tagcgatgtg ctgacgacgt ttttcttttt tgtgattgtg gtgagcctgt ttccgcttgg
     1081 gatagggcca caaccggaca cgctgcgtga aatcgcaccg ggcgtggtat gggtcgccgc
     1141 attgctggcg gcactgttat cgctggcgag aatgttcgat gcagactacg ctgatggcac
     1201 cctggagcaa gtgatgctgg cgccgcagcc accggtcttg atggtattgt cgaagattag
     1261 tgcgcattgg ctgacgaccg gattgccatt ggttgtgatc gcgccgctcc tgggcttgca
     1321 gttcgatctt ccgggacgcg cgcgagccac gctgttgctg tccctgctga tcgcaacgcc
     1381 ggcgctgtcg ttgatcggcg cagtgggcgc cgcattgacg ctcggcgtac gcggcgcagg
     1441 aggcttgagt gcgctgctgg tgctgccgct gtatgtgccg gttctggtat tcggtgccgg
     1501 cgcagtgacc gcatcggccg ccggactaaa tgaagcggcg catttgtcgc tgctcgccgc
     1561 gatggcgctg gtggcactgg tatttgcccc gtgggcaacc gcagcggcat tgcgaatagc
     1621 gctggaataa ttcaggacat gagctggttc aggtattcgt ctcctcagag cttttatccg
     1681 ctagcgggca aaatggtgcc ctggttcttt acgctcgcgg cgctcctcgg cctggcgggg
     1741 ctttatatcg cgttcttcgt tgcgccaacg gatttccagc aaggcgacgc ttaccggatc
     1801 attttcatcc atgtgcccgc cgcatggatg tcgatgttga tctatgcggt gatggctttc
     1861 tgggccggca tcggcctggt attcaatacc aggctgtcgt cgatgatggc gcatgcgctc
     1921 gcccctaccg gagcgctgat gacgctcatc gcactatgga ccggctcgtt ctgggggcgg
     1981 ccgacatggg gtgcctggtg ggtttgggac gcgcggttga cttcagaact gatattgctg
     2041 ttcctgtacc tgggatatct cgcgctggtg gctgccatcg atgatccgcg acgcgccgat
     2101 aaagcttcgg ccgtgctggc gctggtcggc gccataaatg tcccgatcat ttatttttcg
     2161 gtccaatggt ggaacacgtt gcaccagggc tcgaccatta gcatgacgcg cgcgcccagc
     2221 atggccagca tcatgctgat cggcatgctg gtgatggtat ttgcgttctg ggcgtactcg
     2281 attgcggcgg cattgatgcg ggtgcgctgt gaaatcctgg agcgcgagca ccatagtgcc
     2341 tgggcgcgca aattggagga catctaagat gcagtggtca agtgcagggg aatttctggc
     2401 gatgggcggc tatggcctct atgtgtgggg tgcttatgcg gtgactgcgg ttatcatggt
     2461 cgctgaggtc attgcgctgg cgaggcgtgc aagaactctg cgcgcaaaat ccagtcgtag
     2521 tgcggatgca ggcgtggact gaatggcggg gatgaagcca cgacacaagc gcatggcgct
     2581 cattcttgcg ggcctggcga ttctcggtct cgcggctgcg ttggtgctga atgcattccg
     2641 cagcaatctg gttttctttt tcacgcccac acaagtagcg gcacatgagg cgccgcaggg
     2701 ccgcaatttc cggatcggtg gcctggttga aaaaggcagc gtaaagcgtc agcctgatgg
     2761 cgttaccgtg cgtttcatcg ttaccgacac gtcgaagagc gtgccagtta cgtttacggg
     2821 tatccttccc gacctgttca aggaaggaaa aggcgtggtc gcacaaggca agctcggcgc
     2881 cgacggcgtc tttgccgctt ccgaagtact ggccaagcac gatgaaaact atatgccgcc
     2941 cgaagcggcc gatgcggtgg agagggccaa caaggcgatg aaggaatcga agaagactct
     3001 ggtaaagccg tgaatatcgt gaaaaacgtg aatatcgtga atatcgcaac gacacgtgaa
     3061 tgtcgtaaga gccggagcag ttacggcatt tacgaatttc acggcattca cgacattca
//
LOCUS       DATMZQ010000266         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2976675, whole genome shotgun sequence.
ACCESSION   DATMZQ010000266 DATMZQ010000000
VERSION     DATMZQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976675"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..>2009
                     /gene="mutS"
                     /locus_tag="VN496_08730"
     CDS             80..>2009
                     /gene="mutS"
                     /locus_tag="VN496_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXF17077.1"
                     /translation="MTGHTPMMQQYLRIKAQHPDKLLFYRMGDFYELFFDDAVRAARL
                     MDITLTQRGTTAAGPIKMAGVPFHSVEQYLAKLVSVGESIAICEQIGDPATSKGPVER
                     QVTRIVTPGTLTDAALLDDRRDNILAAVLANESALGLAWLSLASGRFVLLETRRQNLG
                     SELERLRPAEILAPDGFEDHALEDSGAVVKRLASWQFDIDSAHQALARHFHTLDLSGF
                     GAETLGPAIGAAGALFEYARGTQAAAIAHVGSLALERDSEYVRMDAATRRNLEISETL
                     RGDAVPTLLSTLDTCITAMGSRWLRHALHHPLRDRKKVSARLHAVGTLADPANMQAYV
                     PIRKALKPVCDVERITARIALRSARPRDLSALRETLAALPSLHALLPVLACPRLDALA
                     DDLGVLPELASLLSRALLAEPAAIVREGGVINDGYDAQLDELRGIQNNCGEFLLELES
                     RERARTGIANLKVEYNRVHGFYIEVTHANAQKIPDDYRRRQTLKNAERYITPELKSFE
                     EKALSANDRALAREKYLYEKLLDALLPSVPVLQRQASALAELDGLAALAERAVALDYC
                     APEFCDDQTLFIEAGRHPVVERQIPEFIANDMCLDTSRRLLLITGPNMGGKSTYMRQA
                     ALIVLLAHVGSFVPARRAM"
BASE COUNT          397 a          604 c          619 g          389 t
ORIGIN      
        1 gaggacggat ttaataactt actgatcagc ggatagcgtc ttgaatacga gtagcaacaa
       61 agatctggtt gaaccggcga tgactggcca taccccgatg atgcagcagt atttgcgcat
      121 caaggcgcag cacccggaca aactgctgtt ctaccgcatg ggcgattttt acgaactttt
      181 cttcgacgac gccgtgcgag ccgcaaggct catggatatc acgctgacac agcgcggcac
      241 cacggcggca ggcccgataa agatggccgg tgtgcctttt cattccgtgg agcagtatct
      301 tgccaagctg gtaagcgttg gcgaatccat cgcaatctgt gaacagatcg gcgatccggc
      361 aacgtccaaa gggccggtgg agcgccaggt cacccgcatc gtgacgcccg gcacgttgac
      421 cgatgcggca ctactcgatg atcgccggga caatatattg gctgccgtgc tcgcaaatga
      481 aagcgcgctc ggccttgcgt ggttgtcgct ggcaagcggg cgttttgtac tgctggagac
      541 gcgtcggcaa aatctcgggt ccgagctgga gcgcctgcgg ccggcagaaa ttctcgcgcc
      601 ggacggcttc gaggatcacg ccctggaaga ttcaggcgcc gtcgtcaagc ggcttgcttc
      661 ctggcagttt gacattgact ccgcacacca agcgctcgct cgccatttcc acacgctgga
      721 tttgtccggc ttcggcgccg agacgctggg tccagcaatt ggcgcggcag gtgcattgtt
      781 cgagtacgcg cgcgggacgc aagctgcggc gattgcgcac gtgggctcac tcgcgctcga
      841 gcgcgactcg gaatacgtgc gcatggacgc agcgacccgg cgcaacctgg agatttccga
      901 aacgttgcgc ggcgatgccg tcccgacgtt gctttcgacg ctggacactt gcatcacggc
      961 catgggatca cgctggctgc gccatgcgct tcaccatccc ctgcgcgacc gcaaaaaggt
     1021 gtcggcaagg ctgcacgcgg tcggaacgct ggcggacccg gccaacatgc aagcttatgt
     1081 accaatacgc aaggcgctca agcccgtgtg tgatgtggag cgcatcactg cgcgcatcgc
     1141 cctgcgcagc gcgcggccga gggatctgtc ggcattgcgt gaaacgctgg ccgcgctgcc
     1201 gtctttgcat gcgctcctgc cagtgcttgc ctgtccgcgg ctggatgcac ttgcggacga
     1261 tcttggtgtg ctgcccgagc ttgcaagcct gctatcgcgc gcccttctgg cggaacccgc
     1321 cgcgatcgtg cgcgaaggcg gtgtcatcaa tgatggttat gatgcgcaac tcgacgagtt
     1381 acgcggcata cagaacaact gcggcgaatt tctgctggaa ctggagtcgc gcgagcgggc
     1441 acgcaccggc atagcaaatc tgaaagtcga atacaaccgc gtgcacggtt tctatatcga
     1501 agtaacccat gccaacgcgc agaagatccc cgatgactat cgccgccggc aaacgttgaa
     1561 aaatgccgag cgctacatta cgccggaact caagagcttc gaggagaagg cgctgtctgc
     1621 gaacgaccgg gccctggcgc gcgaaaaata cctttacgaa aagttgctgg acgctctgct
     1681 gccgagcgtg ccggtcctgc agcgccaggc cagtgcgctc gccgaactgg acggcctggc
     1741 tgcactggcc gagcgcgcag ttgccctgga ctattgcgcg ccggaattct gcgacgacca
     1801 gacgctcttc attgaagccg gcaggcatcc cgtcgtggaa aggcagatcc ctgaattcat
     1861 tgccaacgat atgtgcctgg atacttcgcg ccgcctgttg ctcattaccg gaccgaacat
     1921 gggcggcaag tctacttaca tgcggcaggc agcattgatt gtgttgctgg cgcacgtcgg
     1981 ttcctttgta cccgcgcggc gggccatga
//
LOCUS       DATMZQ010000267         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1250831, whole genome shotgun sequence.
ACCESSION   DATMZQ010000267 DATMZQ010000000
VERSION     DATMZQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250831"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..553)
                     /gene="apaG"
                     /locus_tag="VN496_08735"
     CDS             complement(170..553)
                     /gene="apaG"
                     /locus_tag="VN496_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HXF17078.1"
                     /translation="MAQQKKYEIKVTSSTSHIASQSDEAEQRYVFAYTITILNAGNVA
                     ARLVSRHWIIKDAENRVQEVRGLGVVGEQPLLEPQQKYEYTSSAAISTCIGTMRGSYR
                     MVAEDGVAFDAPIPEFILSAPRVLH"
     gene            634..1293
                     /gene="rpe"
                     /locus_tag="VN496_08740"
     CDS             634..1293
                     /gene="rpe"
                     /locus_tag="VN496_08740"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXF17079.1"
                     /translation="MYRIAPSILSADFARLGEEVSSVLSAGADMIHFDVMDNHYVPNL
                     SMGPMVCKAIRPVTKAIIDVHLMVRPVDRIIPDFADAGANIISFHPEASDHVDRTIAL
                     IKEHGCRVGLVLNPATPLSCLDYTLDKLDLVLIMSVNPGFGGQSFIPAALGKLRAVRE
                     RILQSGRDIWLEIDGGVKIDNIAEIARAGADTFVAGSAIFDSQDYKRTIAAMHAELAR
                     L"
     gene            1378..2037
                     /locus_tag="VN496_08745"
     CDS             1378..2037
                     /locus_tag="VN496_08745"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF009695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HXF17080.1"
                     /translation="MALDLDGTLVDTLPDLHEAAVRMLSDLGKPAVPQATVRAYVGDG
                     VDRLVKRLLTAALDGEPDAGLFERATENFRNHYANVLTHASRPFPGAVTALEKLRSRG
                     FKLACVTNKPARFTEPLLEGLGLNDYLDLVISGDSLPRKKPDPLPLQHVAQRFSIAPA
                     RLLMIGDSPVDTGAARAAGCPVFCVPYGYRGALTMQALDCDAIVPTLLDLLDLIEIPH
                     S"
BASE COUNT          452 a          622 c          624 g          528 t
ORIGIN      
        1 tggcgcgcaa gacgcatagg tcattcatga tctggctcat tctggaagcc gtgctatcgc
       61 tcgtactttt cctgcttatc atctggtgga ctttgccgcg caagaatacg gattccgaca
      121 ataagccatg aggatgaaag cggccgcgag ctttcggaat caagcttgat cagtgcaaga
      181 cgcgtggtgc actgaggatg aactccggta ttggagcatc gaatgcaaca ccgtcttcgg
      241 cgaccattcg ataacttccg cgcattgtgc cgatacaagt cgaaattgca gctgagctgg
      301 tgtactcata cttttgctgt ggttccagca gcggctgctc ccctaccacg cctaaaccgc
      361 gcacttcctg aacccgattt tcggcatctt tgatgatcca gtgccgtgac acgagccggg
      421 cagcaacatt acctgcattc aatattgtga tcgtgtaggc aaagacgtag cgctgctcgg
      481 cctcgtcgga ctggctggcg atatgcgacg tgctggaggt cactttaatt tcgtattttt
      541 tctgctgggc catcgcggca tttgacctga acggccggat ggcggcaagg ccgaaaacgg
      601 tacaatgcgg cctcctcacc agccagtcag attatgtatc gcattgcgcc cagcatacta
      661 tccgccgact tcgcgcgatt gggggaagag gtatcgtcgg tcctctcggc aggcgccgat
      721 atgattcatt tcgacgttat ggacaatcat tatgtgccga atctcagcat gggcccgatg
      781 gtatgcaaag cgattcggcc ggtgaccaag gcaataatcg atgtgcattt gatggttcgc
      841 ccggtggacc ggattatccc ggacttcgcc gacgcgggag ccaatatcat cagcttccat
      901 cccgaagctt ccgatcatgt tgaccgcaca attgcgttga tcaaggagca cgggtgcagg
      961 gttggtcttg tgctgaatcc cgctacgccg ctgtcctgtc tggattacac gctagacaag
     1021 ctggacctcg ttttgatcat gtccgtcaat cccggctttg gcgggcagag tttcattccc
     1081 gcagcgctcg gtaaattgcg tgcagtacgg gagcgaatcc tgcaatccgg tcgcgacatt
     1141 tggctcgaaa tagacggcgg cgtcaaaatt gacaatattg ccgaaatcgc gcgagccggg
     1201 gcggatacgt ttgttgccgg ctctgccatc tttgacagtc aggactacaa gcgaaccatc
     1261 gcggcaatgc atgccgaact agcgaggctt tgaaagcgta gtccgcatgc cagtagcacc
     1321 gatccgtcgc gctcgttagt tctagccgtg cttaactttc cgatttctgt ttcagcggtg
     1381 gcgcttgatc tggacgggac gctggtggac acgttgcccg acttgcacga agcggcggtc
     1441 cgcatgttat cggacctcgg gaaacctgca gttccgcaag caacggtgag ggcatatgtc
     1501 ggcgacgggg tcgaccgcct ggtcaagcgc ctgttgactg cagcgttgga cggcgagccc
     1561 gatgccggac tgttcgaacg ggccacagaa aacttccgca atcactatgc aaatgttctg
     1621 acccatgcgt cccgcccttt tcccggtgcg gtcaccgctc ttgaaaaact gcgttcacgc
     1681 ggattcaagc tggcctgtgt gaccaataag ccggcgcgct ttaccgagcc cctgctcgaa
     1741 gggctggggt tgaacgatta tctcgatctg gtgataagcg gcgatagcct gccacgcaag
     1801 aaaccggacc cactcccgct tcagcatgtc gcccagcgtt tctccatcgc gcccgcgcga
     1861 ttgctgatga ttggcgattc tccggttgat acaggtgcag cgcgtgccgc aggctgtccg
     1921 gtgttttgcg tcccctacgg gtatcgcggc gccttgacga tgcaagcgct cgattgcgat
     1981 gctatagtac ccacgcttct cgatttactg gatctgattg aaattccgca ttcatgaact
     2041 cgttgaaact tcaaaacccg ggtctggaaa ttaaggccgg ctggcgctgg tggcgccagt
     2101 ccgcgtcctg gcgtcgcgac tgacgctaca tcgttcttcc gttccgtttc gaagtttcag
     2161 gagttcatca tgactgagca ggaatttaca cagcttgcag cgcaaggttt caaccgcatt
     2221 ccggta
//
LOCUS       DATMZQ010000268         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_799837, whole genome shotgun sequence.
ACCESSION   DATMZQ010000268 DATMZQ010000000
VERSION     DATMZQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799837"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..938
                     /locus_tag="VN496_08750"
     CDS             147..938
                     /locus_tag="VN496_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF17081.1"
                     /translation="MVEDNEVERGSIIELLGHDDIEIEAVGTGDEALKALLDHPFDCC
                     VLDLRLPDMTGFDLLDRIQAEPALAQIPIVVFTGKDLNPEEEKQLKTVAKSIVLKDVQ
                     SPERLFDETALFLHRVVSELPESKQRMLERLHSSNEVLRNRKVLVVDDDARNIFALST
                     LLENHEIEVLSATNGRQAIDLIQNTENLSAVLMDIMMPEMDGYETMREIRKSVKFRSL
                     PILALTAKAMKGDREKCLEAGASDYIAKPVNTEQLMSMLRIWLYR"
     gene            935..2137
                     /locus_tag="VN496_08755"
     CDS             935..2137
                     /locus_tag="VN496_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF17082.1"
                     /translation="MNAPVLRLPPVNILLVDDKTENLLALESILEAPGYSLVRALSGQ
                     DALLALLAQDYAVIVLDVQMPGMSGIELAQIIRSRKKTQHIPILFLTAHGDESAIAGY
                     QAGGVDFLTKPIQPAILRSKVAVFADLFRKNNALKAEVEERRQAEQRIGQLNKELSER
                     IEELAAANSELESFSYTVSHDLRAPLRQVSGFIALLQESLAERQNEQDAEYIALVQHA
                     VSRMGQLIDDLLRFSRFGRTELNRTSVNLTPLVEQVRETLAPALAGRDVRWKIGLLPE
                     VEGDPAMLRQVFANLIDNALKFTRNKAFAEIEIGAAKEVDEYIFYVRDNGVGFDKRHA
                     KKLFGVFERLHSGGEFEGTGIGLASVRRIIQRHNGRSWAESVPGEGTVIYFSLPLSSA
                     TPTYERLH"
     gene            2121..2645
                     /locus_tag="VN496_08760"
     CDS             2121..2645
                     /locus_tag="VN496_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF17083.1"
                     /translation="MNASIDILLVEDDPGDVHLALAVFRALRIEDRCVVVNDGEEALD
                     YLHARGLFVQRPPGMPRLILLDLKMRRMNGFELLQQIKNDDKLKVIPVVALTSSREER
                     DVERAYDLSVNGYVVKSINFTDYRATLQALAKYWNNVNERPPGFVDRGFAARARADLG
                     EWFRSLCPMPAGNR"
     gene            2661..>3105
                     /locus_tag="VN496_08765"
     CDS             2661..>3105
                     /locus_tag="VN496_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF17084.1"
                     /translation="MAKLARILLVDDTPAEWLLALSGIAEHDGKNETSVVRNKDEALD
                     FLRARGAFRRRASGLPAVVVVGPNVSRPSALALLKEIRADVKLRRVPVVIVATAPDAE
                     MVSRAYEQGVNSLVLNHEDQDIRAERYVELARFWAWANEPPPGCLS"
BASE COUNT          661 a          899 c          942 g          603 t
ORIGIN      
        1 aaattatttc gctggaagaa gagcgccagc acggactgtc gcatggcgcc ttctcctata
       61 tggtcaagcc ggtaacctcc gacagtatcg aggccgcgct ggatcgcatc cgaaactttg
      121 cggccccgca taccaagcgc ctgctggtgg tcgaggacaa cgaggtggag cgcggcagca
      181 tcattgaact gctcggccac gatgacatcg agattgaagc agtcggcacc ggcgatgagg
      241 cattgaaggc gttgctcgat catcccttcg attgctgcgt gctcgatctg cgcctgcctg
      301 acatgaccgg cttcgacctg ctcgatcgca tccaggccga accggcgctg gcgcagatcc
      361 ccatcgtcgt cttcaccggc aaggacctga acccggaaga ggagaaacaa ctcaagacgg
      421 tcgccaaaag catcgtattg aaagatgtgc aatcgcccga acggctgttc gacgagaccg
      481 cattgttcct gcaccgcgtg gtcagcgagc ttccggaatc caagcagcgc atgctggagc
      541 gcctgcattc ctccaacgag gtgctgcgca accgcaaggt gctggtggtc gacgatgatg
      601 cacgcaatat tttcgccctc tccacgctgc tggagaacca cgagatcgaa gtcctcagcg
      661 cgaccaatgg acggcaggcg attgacctga ttcaaaacac ggagaatctg agtgccgtac
      721 tcatggacat catgatgccg gagatggacg gctatgaaac catgcgcgag atccggaaat
      781 cggtgaaatt caggtccctt ccgattctcg cgctgacggc aaaggcaatg aagggcgatc
      841 gcgagaaatg tttggaggcg ggcgcctccg actatatcgc caagccggtg aataccgagc
      901 agttgatgtc catgctgcgg atctggctct accggtgaac gcccccgtgc tccgcctgcc
      961 acccgtcaac atcctgcttg tcgatgacaa gacggagaac ctgctcgcac tggagagcat
     1021 cctcgaagcg ccgggctatt cgctggtgcg cgcgttgagc ggacaggatg cgctgctggc
     1081 gctgcttgcg caggattacg cggtcatcgt gctggatgtg cagatgccgg gcatgagcgg
     1141 catcgaattg gcccagatca ttcgcagccg gaaaaaaacg cagcacatcc cgatcctgtt
     1201 cctcaccgcg cacggcgacg aaagtgccat tgccggttac caggccgggg gagtggattt
     1261 tctgaccaag cccattcagc ccgccatcct gcgttccaag gtcgcagtat tcgcggacct
     1321 gtttcgcaaa aacaatgcgc tgaaggcgga agtggaagag cgtcgccagg cagagcaacg
     1381 catcgggcaa ttgaacaagg aactatccga acgcatcgag gaactggcgg cggccaattc
     1441 ggaactggaa tccttcagtt acaccgtaag ccatgacttg cgcgcacccc tgcggcaggt
     1501 gtccggtttc atcgcgctgc tgcaggagag cctggcggag cgacagaacg agcaggacgc
     1561 ggaatatatt gctttggtcc agcatgccgt cagccgcatg ggacagttga tagacgacct
     1621 gttgcgcttc tcgcgctttg gccgcaccga attgaaccgc accagtgtca atctcacgcc
     1681 attggtcgag caagtgcgcg aaacactggc accggcactg gccggccgcg atgtgcgctg
     1741 gaagatcggg ctgttgccgg aagtcgaggg cgatcccgcg atgctgcgcc aggtgttcgc
     1801 aaacctgatc gacaacgcgc tcaagttcac gcgcaacaaa gcattcgccg agatcgaaat
     1861 cggcgccgcc aaggaggtgg acgaatatat tttttacgtg cgcgacaacg gcgtcggctt
     1921 tgataagcgt catgcaaaaa aactgttcgg cgtgttcgaa cgcctgcact ctggcggcga
     1981 gttcgaaggc acaggcatcg gccttgcgag cgttcgccgg atcatccagc gccacaatgg
     2041 ccgcagttgg gccgaaagcg tgcccggtga aggcacggtc atttattttt ccctccctct
     2101 atcatctgcc actcccactt atgaacgcct ccattgatat tctgttggtc gaagacgatc
     2161 ccggtgatgt gcaccttgca ctcgccgtgt ttcgtgcgct gcggatcgaa gaccggtgcg
     2221 tcgtcgtcaa cgatggcgaa gaagcgctgg actatcttca tgcccggggc ctgtttgtcc
     2281 agcgtccgcc agggatgcca cgcctgattt tgctggatct caagatgcgg cggatgaacg
     2341 gctttgaact gctgcagcaa atcaagaacg acgataaatt gaaagtcatt ccggtcgtgg
     2401 ccttgacctc ctcgcgcgag gagcgtgacg tcgaacgcgc ttacgatctg agtgtcaatg
     2461 gatacgtggt caaaagcatc aatttcaccg actatcgcgc gacgctgcag gcgctcgcca
     2521 aatactggaa caatgtaaac gagcgcccgc ctggattcgt ggaccgcggc ttcgccgctc
     2581 gtgcgcgggc cgatcttggc gaatggttcc ggtcgctgtg cccgatgcca gcgggcaacc
     2641 gttgatccgg agtgcgaagc atggcaaagc tagccagaat tctgctcgtc gatgacacgc
     2701 cagccgaatg gctgctggcg ttgtccggca tcgccgagca cgacggtaaa aatgaaacca
     2761 gcgtggtgcg gaacaaggac gaagcgttgg actttcttcg tgcgcgcggc gccttccgac
     2821 ggcgggcgtc gggattgcct gccgtggtag tcgtgggacc gaatgtgagc aggcccagcg
     2881 ctttggcgtt gctcaaggaa atccgcgctg acgtgaagtt gcgccgcgtg ccggtcgtca
     2941 tcgtcgcaac tgcgccggat gccgagatgg tcagcagggc ctacgaacaa ggcgtgaaca
     3001 gtctggttct caatcacgaa gaccaagata ttcgcgcgga acgttacgtc gagctggcgc
     3061 gcttctgggc ctgggcaaat gagccgccgc ccggttgtct gtcgc
//
LOCUS       DATMZQ010000269         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2629903, whole genome shotgun sequence.
ACCESSION   DATMZQ010000269 DATMZQ010000000
VERSION     DATMZQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629903"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..343)
                     /locus_tag="VN496_08770"
     CDS             complement(59..343)
                     /locus_tag="VN496_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17085.1"
                     /translation="MEERRQAKALVHKNFLEAIRVLLSSDDVKVSDPALLGIYRKGWV
                     ANQQGQYRSFNPHAPESTDWAEWNRGWTDCSKLLRRRQSDNVLGGQRLLR"
     gene            complement(503..832)
                     /locus_tag="VN496_08775"
     CDS             complement(503..832)
                     /locus_tag="VN496_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXF17086.1"
                     /translation="MKTWSNVAAGVAVSILLLGAAGCASEHHRGVSDTGAIASDSWIT
                     TEVKSKLVADKQVSGTAIHVNTYEGVVTLTGKAKSQEEANRAVEDAKSIKGVKSVENN
                     IEVASNS"
     gene            complement(1258..>2517)
                     /locus_tag="VN496_08780"
     CDS             complement(1258..>2517)
                     /locus_tag="VN496_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXF17087.1"
                     /translation="TYISKIRWTPRFDGFAISFEARIKGRQTEDLQVDVTVRHGNRIL
                     ARDSYQVIDQEVNRRVFLADPGIDDFRNELLWSPERPTLLDAEIRLKSAGVVIDTLRS
                     YTALRDVSISRDRFLLNGRPYLLRMVLDQGYWPDTLLAAPNDEALRRDVVLAKAMGFN
                     GVRKHQKIESPRYLYWADKLGLLVWAEMPSAYRFTRTAIKRLVREWSEVIARDYSHPC
                     VIVWVPFNESWGVPDLPSISAHRHAVEALYHLTKTLDPTRPVIGNDGWESSATDIIGI
                     HDYDASAERIRQRYGPEADTQHLFDRRRPGGRILTLDGYPHRGQPIMLTEFGGMSYVD
                     GPQQGVHKPWGYSFAETRDEFARHYVDLMNVLADTPLFSGFCYTQFADTFQETNGLLT
                     ADRKPKFPLEEIAKATQTSRSYIPGWV"
BASE COUNT          524 a          734 c          664 g          595 t
ORIGIN      
        1 tggtgagaac tgctttgagg tttacctgct gttgttattt gtagtctcct gccttgctct
       61 atcgaagcag ccgttgccca cccaaaacat tatccgactg ccgccttcgc aagagcttgc
      121 tgcaatcggt ccagccgcga ttccattctg cccaatcagt cgattccggt gcatgaggat
      181 taaatgaccg gtattggcct tgctggtttg cgacccagcc tttgcgataa atacccagca
      241 gagccgggtc gctgactttt acatcgtcgc tactgagcaa tacccgaatg gcctcgagga
      301 agtttttgtg cacgagcgct ttcgcctggc gtctttcctc caccgcggga gctccttatg
      361 acgatcgttc cttcgacttc gcccgccaac acaaaatccc gcgtgagctg atccgattgg
      421 aggctcagcc aaacaacggc tggaaataaa aagggcggaa ctgatgtccg cccttgttcg
      481 cgagttgcct cacccgctgt gcttacgaat tgctggcgac ttcgatattg ttttcgaccg
      541 atttgacgcc cttgatggac ttggcatcct ctaccgcacg atttgcttct tcctggctct
      601 tcgcttttcc ggtcagcgtt acgacaccct cgtaggtgtt gacatgaatg gcggtgccac
      661 tgacttgctt gtcggcaacc aatttggatt tgacttcagt cgtaatccac gagtcgctgg
      721 caatagcacc agtgtcactc acgcctcgat gatgttcact tgcacatcct gcagcaccga
      781 gcaacaggat tgaaaccgca actccagcag ccacattcga ccacgtcttc atgctcgttc
      841 tccttggaaa aaagtaggca acgcacttgc ctcattcgac cgccgtgaaa tcagcacaaa
      901 gttgtttccc caacttccag gcctggtcgg tggacgtact tatgcaaagg ctgtgccctg
      961 cacgctgcgc agacaagaat gctgaccgtt caatgccttg tgattaacag caagcgtcga
     1021 ctctttagga cggctgcatt ttttacatgg tggttgggtc attacttcca aggcgcaagc
     1081 cagtatttgc gcgatgccag tcaaataata gccgcccaaa cctagtcttc tcgggccatg
     1141 tccgactgca cgatgcgtag gcggagtttg agatcgtgga gtccccctta gggtacttcc
     1201 atgtgtaccc tgtttgccgc accgggcacc cggtgcggct ttttctttgc gcggtactca
     1261 gacccacccc ggaatgtagc tgcgcgacgt ttgcgtggct tttgcaattt cctccaaagg
     1321 aaatttcggc ttgcggtcag cagtgagcaa tccgttcgtt tcctggaaag tgtcggcaaa
     1381 ctgtgtatag caaaatccgc tgaacaacgg cgtgtccgcc agcacattca tcaagtcaac
     1441 ataatgccgg gcaaactcgt ccctcgtttc cgcaaacgaa tagccccacg gcttatgcac
     1501 gccctgctgc ggaccgtcga cgtaggacat gccgccaaat tcggtcagca tgattggttg
     1561 cccccgatga ggatagccat ccagcgtgag gattctgccg cccgggcgac ggcgatcgaa
     1621 tagatgctgt gtatcggctt cgggaccgta gcgctgtcgt atgcgctcgg cactggcatc
     1681 ataatcatgg atgccgatga tatcggtggc actgctttcc catccatcat taccgatgac
     1741 cgggcgcgtt ggatcgagcg tcttcgtcag atgatagagc gcttcgaccg catggcggtg
     1801 cgcactgatc gagggtaagt ccggcacgcc ccacgattca ttgaacggaa cccacacgat
     1861 tacgcaagga tggctatagt cgcgggcaat cacctccgac cattcgcgca cgaggcgctt
     1921 gatcgccgtg cgcgtgaatc gatatgcgga aggcatttct gcccatacca gaaggccgag
     1981 tttatccgcc cagtaaaggt aacgcggact ctctatcttc tggtgcttgc gcacgccgtt
     2041 gaagcccatg gccttggcca gaacgacatc gcgcctcagc gcttcatcgt tgggagctgc
     2101 gagcaacgta tccggccaat acccctggtc gagtaccatg cggagcagat acggccgccc
     2161 gttcagcaga aagcggtcac gcgaaatgct gacatcgcgc agggcggtgt aggaacgcaa
     2221 cgtgtcgatc accacaccag cgctcttcag gcggatttct gcatcgagca aggtcggccg
     2281 ctccggactc cataacagtt cgttgcggaa atcgtcaatc ccggggtcgg caagaaacac
     2341 cctgcggttc acttcctggt cgatgacctg gtaggaatcg cgcgccagga tgcgattgcc
     2401 gtggcgcacc gtcacgtcga cttgcaaatc ctcggtttgc cttcctttga tgcgagcctc
     2461 gaagcttatg gcaaagccgt cgaagcgagg cgtccagcgt attttcgaaa tataggt
//
LOCUS       DATMZQ010000270         6520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4019236, whole genome shotgun sequence.
ACCESSION   DATMZQ010000270 DATMZQ010000000
VERSION     DATMZQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019236"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VN496_08785"
     CDS             <1..862
                     /locus_tag="VN496_08785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF17088.1"
                     /translation="ELTIVAPLSPGKPRLAAVEHGQKPARSAPVATLPKAEPLRDPID
                     GIPSQAEIVPKPVQQIRSEKPVKDINTPGPTAAATPPAAIDKQMREVAPSEGAEISFR
                     KGVAQIQEGRANKAELEFRDALKQDPSHAGALQALLNLLLASGRNNDAEQLLRTALES
                     DSHQPRLGMILARLEVERGEVPGAINTLVGVLPYVQSDSEFYAFLAALLQREGRHKEA
                     VEYYRAALRTVPGNGVWTMGLGISLRASNQGAEAREAFTRAADSGQLSPELQQFVERQ
                     LRELSLQKKK"
     gene            complement(865..1587)
                     /locus_tag="VN496_08790"
     CDS             complement(865..1587)
                     /locus_tag="VN496_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXF17089.1"
                     /translation="MSNPLNQILDYCLPVVRWIIAPACPLCGGQANHDGLCVGCQASL
                     PQLPHAQCPVCAIGNSTAELCGRCISARPFFDRVVAPFTYGFPVSVLIQGLKYRGELA
                     CAKPLAAAMANVLDEEPYPDLLIPMPLSHTRLAARGFNQAMEIARYIAAEFGLHIAVD
                     ICRRNGDGTPQTALPWKERASNVRNAFTCHENLEGKSVAVVDDVLTTGATLNELARTL
                     KRSGAREVTGWVAARTPAPGET"
     gene            1640..2644
                     /gene="bioB"
                     /locus_tag="VN496_08795"
     CDS             1640..2644
                     /gene="bioB"
                     /locus_tag="VN496_08795"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HXF17090.1"
                     /translation="MPQAEQTFITDSFHQSALAWDPPRVETLLSTPFADLIYQAQHVH
                     REHFAANEVQLSTLLSIKTGGCPEDCHYCPQAARYGTGVESQELMSVEAVVEAARSAK
                     HNGASRFCVGAAWRGPKQRDLEKVAEMVREIKALGLETCATLGMLKEGQAQQLKQAGL
                     DYYNHNIDTDPAFYRDIVTTRQQGDRLDTVRKVRAAGIHVCCGGIVGMGETRANRAAL
                     IAELANLDPYPESVPINNLVQVKGTPLHGTPLLDRFEFVRTIAAARITMPRAMVRLSA
                     GREDMSEEMQALCFLAGANSIFYGEKLLTTSNPLADGDRKLFSKLGISPMASAPGTTG
                     "
     gene            2761..3612
                     /gene="bioC"
                     /locus_tag="VN496_08800"
     CDS             2761..3612
                     /gene="bioC"
                     /locus_tag="VN496_08800"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HXF17091.1"
                     /translation="MLDKRQVRRSFEHAASTYDHAAVLQREVATRALERLELVRINPS
                     VIVDAGCGTGFALPSLHQRYPRATLVALDIAFAMLQKSRARIPGWKRWSGLSKAVFVC
                     GDNETLPLQAACAEMLWSNLSFHWANDLAALFAECRRILTPGGLLMFTTFGPDTLKEL
                     RRAWAVDSNTHVNRFIDMHDIGDMMVAAGFSDPVMDMEYLTLTYNDIRALMRELKAVG
                     AHNVAIGRSRGLTGKHSFQHIEMRYESLRQSGRLPATFEVIYGHAWKPAPRLTPEGRQ
                     IIDIKAS"
     gene            4137..4841
                     /gene="coxB"
                     /locus_tag="VN496_08805"
                     /pseudo
     CDS             4137..4841
                     /gene="coxB"
                     /locus_tag="VN496_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
     gene            4914..5171
                     /locus_tag="VN496_08810"
     CDS             4914..5171
                     /locus_tag="VN496_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF17092.1"
                     /translation="MAGKATGDGKGTYEKVCSVCHAAGVAGAPKFGDKSAWAPRIATG
                     ADALHNSALKGKNAMPPKGGMTTLADADVVAAVDYMVKAAK"
     gene            5234..>6520
                     /locus_tag="VN496_08815"
     CDS             5234..>6520
                     /locus_tag="VN496_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXF17093.1"
                     /translation="MSAVIGHDHGHAHGHDYHSGGILRWVKTTNHKDIGTMYLWFSFI
                     MFLTGGVMAMVIRAELFMPGMQVVNPEVFNQMTTLHGLIMVFGAIMPAFVGFANWLIP
                     MQIGAPDMAFPRLNNWSFWLLPPAALLLIISLFVPGGAPGVGWTLYAPLSVQQGMGMD
                     LTIFAVHILGMSSIMGSINIITTVLNLRAPGMTLMKMPLFVWTWLITAYLLIAVMPVL
                     AGAVTMTLTDRHFGTHFFNAAGGGDPVLFQHIFWFFGHPEVYIIAIPAFGVVSQVIPA
                     FSRKPLFGYTSMVYAVASIAILSFLVWAHHMYTTGLPVTGQLYYMYATMLIAVPTGVK
                     VFNWVATMWKGSLSFETPMLFSIGFLFLFTLGGFSGLVLSIVPVDIQLHDTYYVVAHF
                     HYVMVAGALFSAFAGIYFWLPKWTGHMYDETLGKWHF"
BASE COUNT         1406 a         1793 c         1887 g         1434 t
ORIGIN      
        1 tgagttgaca atcgtcgccc cactttcgcc gggcaagcca agattagctg ccgtggagca
       61 cgggcagaaa cctgcccggt cagctcctgt cgcgacattg cccaaagcgg aacccttacg
      121 tgacccgatt gacggcatac catcgcaagc cgagatcgtg ccaaagccgg tccagcagat
      181 tagatccgaa aagccggtca aggatattaa tacgcccgga cccacggccg ctgctactcc
      241 acccgcggca atagacaagc agatgcgcga agtggctcct tctgaaggcg ccgaaatttc
      301 tttccgaaaa ggggtcgcgc agattcagga aggtcgcgcg aacaaagctg aactggaatt
      361 ccgggatgca ttaaaacaag atccatcgca cgcgggagct ctgcaagcct tgctgaatct
      421 attgctggcc agcgggcgca acaacgatgc ggaacagcta ttgcgcactg cgctggaaag
      481 cgattcgcat cagccgcgcc ttgggatgat cctggcgcgt ctcgaagtgg agcgtggaga
      541 agtgcctggc gcaatcaaca cgctggtcgg cgtgctgcct tatgtgcaat cggattctga
      601 gttctatgct ttcctcgcag cgctgctgca acgcgagggc cggcacaagg aggccgtaga
      661 atattatcgt gccgcattac gtactgtccc cggcaacggc gtttggacga tggggcttgg
      721 catatccttg cgggcctcaa accagggggc agaggcgcgc gaagcgttta cgcgcgccgc
      781 tgattccgga caattgtcgc ccgaattgca gcagttcgtg gaacgtcagc tgcgagagct
      841 ttccctgcag aagaaaaaat aagttcaagt ttcgcctggc gctggcgtcc tcgctgcgac
      901 ccatcctgtt acctcgcgcg caccgcttcg cttcagtgtc cgcgccagct cattgagcgt
      961 ggctccggtg gtcaaaacat catccactac tgcaaccgat tttccttcga gattttcatg
     1021 gcaagtaaac gcatttcgga cgtttgatgc gcgttctttc cacggcagcg ccgtctgcgg
     1081 agttccgtcg ccgttacggc ggcatatatc cacggcgatg tgcagcccga attccgcggc
     1141 gatgtatcgc gcgatttcca tggcctggtt aaatccgcgg gccgccaacc gggtatgact
     1201 caatggcatc ggaatcagca aatcggggta gggctcttca tcgaggacat tggccatggc
     1261 agccgcaagc ggttttgcgc aggctagctc accgcggtac ttcaatccct ggatcagcac
     1321 agaaacggga aaaccgtaag tgaagggagc aacgacccga tcgaagaagg gtcgcgcgct
     1381 gatgcagcgc ccacataact cggcagtgga gttgccgatc gcacaaaccg gacactgcgc
     1441 atgcggcaac tgaggcagac tggcttggca gccaacacat aagccatcat gattggcttg
     1501 tccgccgcac agcggacaag ccggagcgat gatccatcgg acaaccggca ggcaataatc
     1561 cagtatttga ttaagtgggt ttgacactgg tgcagccttc ccggaccatt ctccatccca
     1621 agtagaacac taaaaaatca tgcctcaggc cgagcagaca ttcattaccg acagcttcca
     1681 ccaatccgct ttagcctggg acccgccacg cgttgaaacg ctgttgtcta ctccgttcgc
     1741 agatctgatc tatcaagcgc agcacgtcca tagggagcat tttgcggcta acgaagtaca
     1801 actttctacc ttgctttcca tcaaaaccgg gggttgtccg gaagattgcc attattgtcc
     1861 gcaggccgcg cgctatggca cgggggttga gtcacaggag ctgatgtcgg tagaagcagt
     1921 agtcgaggcc gcgcgcagcg caaaacataa cggggcttcc cgattctgtg tgggtgcagc
     1981 ttggcggggt ccgaagcaac gcgatttgga aaaagtcgcg gagatggtgc gcgagatcaa
     2041 ggcgctaggc ctggagactt gtgcaacgct tggcatgctg aaggaggggc aagcgcagca
     2101 gctcaagcaa gccgggctgg actactacaa ccacaacatt gatacggatc cagcctttta
     2161 ccgtgacatc gtgactaccc gccagcaggg cgatcgcctg gacaccgtgc gcaaagtgcg
     2221 cgcagccggt attcacgtgt gttgcggggg aattgttggc atgggagaaa cgcgggccaa
     2281 ccgcgccgca ctcattgccg aactggcaaa tttggatcca tacccggaaa gcgtgcctat
     2341 taacaacctg gtacaggtca agggaacacc tttgcatgga acaccgctac tcgatcggtt
     2401 cgaatttgtg aggaccattg ccgcagcacg cattaccatg ccgcgtgcga tggtaaggtt
     2461 gtctgccggg cgtgaagaca tgtcggaaga gatgcaggca ttgtgctttc tggctggtgc
     2521 caactccatt ttctatggcg aaaagctgct taccacgtcc aatccgctgg ccgatggcga
     2581 tcgaaagctg ttttccaagc ttggcatcag ccccatggcc tcagccccgg gcacaacggg
     2641 ctaggttggt tcgctcgcta tgcgcggatg cctgtccgct gcgcataagc tctcggcaag
     2701 gtgcgttaca taacccaaag aatgcaagcg acgagctgcc attttatgat tgattggagc
     2761 atgctcgaca aacgccaggt acgcagatcg ttcgaacacg cagcatcgac ttacgaccac
     2821 gctgcggtcc tgcaacgcga ggtcgcgacg cgtgcgctgg agcggctgga attggtcaga
     2881 atcaatcctt ccgttatcgt ggatgccggt tgtggtactg gcttcgcttt gccgtccttg
     2941 caccagcgct acccccgagc gacactggtt gcgttggata ttgcttttgc catgctgcaa
     3001 aagtcgcgcg cgcgcattcc aggatggaaa agatggagcg gattgagcaa ggcggttttt
     3061 gtttgcggcg acaatgaaac gctgcctctg caggccgcct gtgcggaaat gctatggtcc
     3121 aacctttcct tccattgggc caatgacctg gcggcgctgt ttgcggaatg ccggcgcata
     3181 ctgacacccg gcggcctgct gatgttcacg accttcggtc cagataccct gaaggaattg
     3241 cgcagagcat gggcggtgga tagcaatacc cacgtcaacc gcttcatcga catgcacgac
     3301 atcggcgaca tgatggtcgc cgctggattt tccgatccag tcatggacat ggaatatctg
     3361 accctcacat ataacgacat acgcgccctt atgcgcgagc tgaaggccgt tggtgcgcat
     3421 aacgtcgcta tcggccgtag ccgcggattg accggaaaac acagctttca gcacattgag
     3481 atgcgctatg aatctttgcg gcaaagcggg cgtttaccgg ccacttttga agtcatctac
     3541 ggccatgcct ggaaaccagc accgcgcctg actcccgagg gcagacaaat catcgatatc
     3601 aaggcaagct gaaaggcagt gggagtttgg tactgaagct ggctgctttc ttgccatgga
     3661 accggcgctt gtgcgacaat cgcccgcgct taaaaggggc ggagttgtgt ctttgcgact
     3721 ggcagaggca cgcaaaaaca ggattcggca tgaactgcga atgaacgcaa caacggtgga
     3781 gattccggcg gggttcatct tcgtctcacg gcgcaacgat tccctctctc gtcgttcagc
     3841 agtttccccg cggtttgcat taatccttcg cgatttccct tggcttcgca ttgcatcaat
     3901 aaaagagcaa cgtgcgaagc gcggtcgcgg gttgttgctg gcaaagaatt tggagttctg
     3961 cgtcaccgga gttggcgggg cagcgcgcca aaatcgcgtg tgactcagaa caccggagaa
     4021 tccgttaata caggtattcg tttgggggaa gcatgatcaa gacgttatgc gccaaaattg
     4081 gcatctgcgg gttgacgatg ctgataccgg gtttggcttg ggcagaggac aaggccaagt
     4141 ggaatctgca agagccgcac actgcggtcg cgcgggaaat gtacgatctt cacacgatcg
     4201 tgctggtcat ttgtctaatc atcttcgttg gcgtgttcgg cgcaatgttt tattcggtga
     4261 tcaagcaccg aaagtccgcg ggccaccggg cggcccattt ccatgagaac accaccgtcg
     4321 aaattctatg gaccatcatc ccgtttttta tcctgatcgg catggcatgg ccggcgacca
     4381 aaagcgtgct ggcgatgcgc gatacgtcgt cgcctgacat gacgatcaag ataaccggct
     4441 accagtggaa atggggctac gagtatctaa agggagaggg cgatctttcc ggcattgctg
     4501 acggcgtcca gttctatagc gtgctggcaa cgccccacga ccagatctat ggcaatgcgc
     4561 caaaaggcga acattatttg ctcgaggtcg acaatccact cgtggtgccg gccggcaaga
     4621 agatacgcct tttgattacg gccaacgatg tgctgcacgc ctggtatatg cctgccttcg
     4681 gcgtaaaaca ggatgccgtg cctggattca ttcgcgatgc ctggttcaag gtcgaccagc
     4741 caggcacata ccgtgggcag tgtgcggagt tgtgcggcaa ggagcatggc ttcatgccga
     4801 ttgtcgttga ggcccttgag cctgcgaaat ttgccgaatg ggccggtagc cataaaacgg
     4861 ccgcagcgcc tgcggccacg gcggtagccg ccgcagctgg cccggaaaaa actgtggcag
     4921 gcaaggcaac gggagacggc aagggtactt atgaaaaggt ctgttcggtc tgtcacgctg
     4981 ctggcgtggc gggcgcccca aaattcgggg ataagagcgc gtgggcaccg cgcattgcca
     5041 ccggtgccga tgctctgcac aacagtgcgc tcaagggtaa gaacgcgatg ccgcccaagg
     5101 gaggcatgac tacattagcg gatgccgacg tagtagcagc agtcgactac atggtcaaag
     5161 ccgctaagta gccgccgcgc catcgaacag gatcaatgtt aagaaattcg ccaggcatcc
     5221 aggagaacca agcatgagcg cagtcatcgg ccacgatcac ggacatgccc atgggcatga
     5281 ttatcattca ggcggcatct tgcgttgggt caagaccacg aaccacaagg atatcggaac
     5341 gatgtacctg tggttcagtt tcatcatgtt cttgaccggc ggcgtcatgg caatggtcat
     5401 ccgcgcagaa ttgttcatgc cgggcatgca agtagtgaat ccggaagtgt tcaatcagat
     5461 gaccacgctg catgggctga tcatggtgtt cggagcgatc atgcccgcct tcgtcgggtt
     5521 tgcgaactgg cttataccca tgcagattgg tgcacccgac atggcgttcc cgcgtctcaa
     5581 caactggagc ttttggttgc ttccgccggc ggcacttctg cttatcatct cgctgtttgt
     5641 tcctggcggt gcacccggcg tcggttggac tctttatgcc ccgctatcag tccagcaggg
     5701 gatgggcatg gatctgacga tcttcgccgt ccatatcctc ggcatgtcgt cgatcatggg
     5761 ttcgatcaat atcattacca ccgtcctgaa cttgcgcgcg ccgggaatga cgttgatgaa
     5821 gatgccgctt ttcgtgtgga cgtggctgat cacggcctac ctgctgattg ctgtaatgcc
     5881 ggtacttgct ggtgcggtga cgatgacgct taccgaccgg catttcggaa cccatttctt
     5941 taatgcggcg ggcggtggag atccggttct gttccagcat atcttctggt ttttcggcca
     6001 tcccgaggtt tacatcatcg ctatcccggc ctttggggtc gtctcgcaag tgattccggc
     6061 attttcgcgc aagcctctgt tcggctatac ctccatggtg tacgccgtgg catccattgc
     6121 gatactttct ttcctggtct gggcacatca catgtacacc acgggccttc cggtgaccgg
     6181 tcagctttat tacatgtacg cgaccatgct gattgcggtg ccgaccggag tgaaagtgtt
     6241 caactgggtg gccacgatgt ggaaaggctc gcttagcttt gaaaccccaa tgctgttctc
     6301 gattggattt ttgttcctgt tcacgcttgg cgggttcagc ggactggttc tgtccatcgt
     6361 gccggtcgat atccagctgc acgataccta ttacgttgtc gctcatttcc actacgttat
     6421 ggtggcggga gccctgtttt cagcttttgc cggcatttac ttctggttac cgaaatggac
     6481 cggccatatg tacgacgaaa cgctgggcaa atggcatttc
//
LOCUS       DATMZQ010000271        13497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1258211, whole genome shotgun sequence.
ACCESSION   DATMZQ010000271 DATMZQ010000000
VERSION     DATMZQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258211"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VN496_08820"
     CDS             <1..613
                     /locus_tag="VN496_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17094.1"
                     /translation="LGTPIARLGRLAVAPDGAVWFAESTTYGFTRLKDGVLTRNVPKS
                     VRGGPYGVAVDSKGDVWGTLQSGNQLVKISQGKDIAEYELPTPGSSPSDVAADSAGNV
                     WLLEFRSNKIAKFSNGKFSEFVVPGEWSGLSGIATATDGSVWFGLLRNHAIGRLRNGE
                     FKIFELPRKDARPYTLAIDKDSNVWYADISGYVGMLKADAATR"
     gene            complement(671..1603)
                     /locus_tag="VN496_08825"
     CDS             complement(671..1603)
                     /locus_tag="VN496_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17095.1"
                     /translation="MTDALIYLASAFWIGAVHAATPGHGKTIAAAYIVGARGKPVDAV
                     ILGIFVTLSHTSGIVLVGILASIGMPGMVPQRIEAWLALATGLLVVIIGLWTLWTQRD
                     LIMLLQGVPRTTSSGLLHTDSATEQLQPHPAGINHAPMQFRPHTHSQEHQFSHSHHHG
                     QHSHRGAAHTHTHSGDHQHDEEAGYHSHGWGFKHTHDMKLATTNRPGLWILIGLGVAG
                     GLLPDPAALAILLNALAQGKVMLGLGTVVVFSIGFAGTLVVVGVVAAKVGQRILDWLA
                     GPWAMRLQVGTSLLIVIVGTILSFGAWRTLSTLT"
     gene            complement(1858..3036)
                     /locus_tag="VN496_08830"
     CDS             complement(1858..3036)
                     /locus_tag="VN496_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXF17096.1"
                     /translation="MRITDVRTIPVEVKLPKPVYDANYTMATKPALLVEVETDQGLVG
                     LGEAAHFGGPMQSTSQVIENELRDYLIGQDPRNIEYLWEVMHRRAYKHARGGIVIAAI
                     SGIDIALWDLRGKMAGMPLWRLLGGYRQTVPAYATGGFYSVGKGIKELALEMETYVKH
                     GFRAVKMKVGRNPGIELSPLRAMDNASECLVALADDIARVAAVREAIGPDIKLMVDAN
                     AAWDVPTAVRMGRAMQGYDIYWYEEPVSPDDVAGSVEVAAKVDIALAGYETCSYGIVD
                     FNDYIKARAVHFVQPDVAWAGGLTETLKIAHMAQAANLPIAPHIHGSAVAVAAAVHLL
                     GAVRNGSMAETVFPAHALMNELVKEPLLVDKNGNITLSERPGMGIELEPKIVKKYLAI
                     "
     gene            3603..4460
                     /gene="otnI"
                     /locus_tag="VN496_08835"
     CDS             3603..4460
                     /gene="otnI"
                     /locus_tag="VN496_08835"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HXF17097.1"
                     /translation="MPKFAANLHYLFTELPFLQRFEAAANAGFEAVEFQVPYDYPETE
                     LSARLKGNKLQMVLFDAPMGDWARGDRGLAAVAGRESEFRASLARVIEYGNALNCDTV
                     HLMAGVIGASEDLHNAEHTYIANLRHAAPLLKEHGITAVIEPINKKMGISKDAPSYTT
                     HGMHGYFLNHTEQARRIIQAVGSPNLLLHLDCYHMQMLEGHLSETFRDNIALLRHVQV
                     AGVPGRHEPTVGEINYPYLFNLLDELSYRGWIGCEYRPKGDTWSGLSWASAYGIRADR
                     SDNHKGRAG"
     gene            complement(4554..4703)
                     /locus_tag="VN496_08840"
     CDS             complement(4554..4703)
                     /locus_tag="VN496_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17098.1"
                     /translation="MKSLFDSLLRLYEFDGPPPAVSSATAQKVSEEQLPDLSNQDAST
                     FPYLP"
     gene            4817..5767
                     /locus_tag="VN496_08845"
     CDS             4817..5767
                     /locus_tag="VN496_08845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXF17099.1"
                     /translation="MDRLYQMRLFVRIVETGSFSAVARELGTIQPTVSKQLTALEEHL
                     GVRLLNRTTRQLSTTEAGRKYYDRCRHILDEIIDLEGSLTELQNSPTGLLRINAPVAF
                     GQLYMLPLVFRFRRLFPGLAIDLTLNDRYVDLVQEGIDVAIRFGELSDSQVVARSVGR
                     VTRVCLASPAYLRARGVPKTPNDLLSHNCITYNYLFTNEWQFGGPEGPTTLRVAGDFR
                     ANSALTIRTAALEGIGIANMPSVFVQNDLDNGSLIRVLADFAAPPVEIHALYPSARFL
                     SSKVRLFLDFVRDEFLQIASLQVAAIKPKRIPRIRAKVTG"
     gene            5875..7863
                     /locus_tag="VN496_08850"
     CDS             5875..7863
                     /locus_tag="VN496_08850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HXF17100.1"
                     /translation="MPKKPNAYTAEDIEILEDLSPILHRPGMYTDPASPNHALVEIID
                     NAADEGLAGFAKSVEVTLYEDGSAQVTDDGRGIPVDIHPKKKVPAVQVIFTTLHSGGK
                     FRKTDKDAAYRIAGGLHGVGVCVPNALSTRLEVEVKRDGEKSRIVFADNGKVKEPLRK
                     TATIGPRDSGTRVRFWPDPKYFDSPKIVAAEIAALLRAKAMLLPGVKFSLATERNKKF
                     EVQTWHFPEGIKGYFASAMQGQEPVAETFLGERYVAAQSESDNFAEGEGAMWAIAWTP
                     EGEPVAESYVNMIPTRIGGTHVAGLREAVYNAIKNFIDLHAMGQRGLKIVPEDVWSRT
                     SFVLAIKMLDPQFKGQVKNELISRDAVKLTAQMVRDPFELWLNQHVDEGKRIAELVMS
                     QANARTRAAQKIERRKLSGVAVLPGKLTDCASEDPERNELFIVEGDSAGGSAKQARDK
                     EFQAVLPLKGKPKNTWQDSADTLYANKEIEAIALAIGVDHHKSTDTVDLAGLRYRKVI
                     VLADADVDGAHIQVLLLTLFFRHFPKLIEGGNIYVAQPPLFRIDVSSQGKGKPARKLY
                     ALDKQELDGIEEKLEDEGFKPNSWTVSRFKGLGEMNPEQLWETTLNPDTRRMLPVRVD
                     DGANISVDIFNMMMSRENASQRREWMETYGNLVEADMS"
     gene            8070..10355
                     /gene="parC"
                     /locus_tag="VN496_08855"
     CDS             8070..10355
                     /gene="parC"
                     /locus_tag="VN496_08855"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXF17101.1"
                     /translation="MSGDLGDSLPLGKYASTQYLQYAIATVKDRALPRVGDGQKPVQG
                     RILYSMWTNNNRSGTKRTKSAAVVGEVLGKLHPHGDQSVYDALVRLAQDFTMRYPLID
                     GEGNFGSRDGDEAAAYRYTEARLTKFAEVLLAEIDMGTVDFSQNYDGTREEPTILPAR
                     LPVLLLNGASGIAVGMATEIPSHNLNEVAAAAIALIRDPELNITGLMKHIKGPDFPAG
                     AQIITPRAELKEAYTTGRGAVRVRARWNIERLARGQWQMVVYELPPGASAKKVLEEID
                     GITNPQSKPGKKSLTPEQTREKQLMLSMLDKARDESDRTHPVRLVFEPKTSRIDETEF
                     VNLLLAKTGMEGNVPINLVMVGLDGRPTGKNLRDILSEWIRFRFTTVTRRTRHRLTQV
                     LDRIHVLEGRMIVLLNVDKAIRIIRNADEPKPELIKAFKLSERQAEDILDIRLRQLAK
                     LEQIRIEQELKELKKEQRSLEKILKDPKVLGELVTSEIEADVKTFGDKRRTRIEESEK
                     AAIETPIIDEPVTVIFSTKGWVRARQGWEVEAASLSFKEGDGLMSLIKCRTIDPVIFM
                     DSRGRTYTVEAGQLPPARGDGAPASSLVEVQDGAHILYCLSGKPDTKVLVASTAGYGF
                     LSSVGDMISSRKAGREFMAVDDGHTPLPPYVYQEQPGNYVASLSEGGRLLLFAVDELK
                     MVARGRGMIVMGLDDGEKLAAVAVSNKQTLMVTGIGQRTAKEKTILLSGQKLAHHILR
                     RARMGRVLPEKLKMPLRIAVE"
     gene            10461..11384
                     /gene="cysK"
                     /locus_tag="VN496_08860"
     CDS             10461..11384
                     /gene="cysK"
                     /locus_tag="VN496_08860"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXF17102.1"
                     /translation="MKADNILGTIGNTPHIRMNRLFGTGQNVWIKSERANPGGSIKDR
                     IALSMVEDAEKRGKLKPGGTIIEPTSGNTGIGLALVGAVKGYAVILVMPDSMSVERRR
                     LMLAYGAKFELTPREKGISASIARAQELLAEIPGAWMPQQFENAANTEVHARTTAREI
                     LADFPEGMDLLITGVGTGGHITGCAQVLKKAWPKLKVFAVESALSQVIAGGKHSPHPL
                     QGIGTGFIPKILDTALLDGTIPVEAEAAKEMARRAAREEGMLVGLSSGATLAAIAQKL
                     PEFPKGTRVIGFNYDTGERYLSVEGFLPTGA"
     gene            11531..11983
                     /locus_tag="VN496_08865"
     CDS             11531..11983
                     /locus_tag="VN496_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXF17103.1"
                     /translation="MQPVLAWLLAGLLLVVVELMTGTFYLLILGIAAGIGSVIAFLEQ
                     PFWMQALAAAISAIVGGVLVNRYHRAANASSPRDSSNDIGEPVSIESWVSEPNRLARV
                     RYRGTTWDAEVIGTERIEADALLYVVATDGSRLKISSSRPPEHHHKHK"
     gene            12042..13001
                     /locus_tag="VN496_08870"
     CDS             12042..13001
                     /locus_tag="VN496_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXF17104.1"
                     /translation="MYPPTHGYAVPFFVIALAVVFVIEGVKVVPQQNAWVVERLGKFN
                     AILEPGLNFIMPFVDRVEYRHSLKEVPLDVPEQVCITKDNTQLSVDGIIYFQVTDPRL
                     ASYGTSNYVFAITQLAQTTLRSEVGKMELDKTFESRDEMNKQVVSVLDEAGRTWGVKV
                     LRYEIKNLTPPEAILRSMQAQITAEREKRALIAKSEGQRQQEINLADGEKQAKILSSE
                     GDKTAQINKAQGEAGAIKMIAEAQAEAVRVVGQAIGTAEGLSAANLKVAELYVGAFKE
                     LAKANNTLIIPSNLSDAASFVASAMTVLDRAKLGTETQKAAQA"
     gene            13011..>13497
                     /locus_tag="VN496_08875"
     CDS             13011..>13497
                     /locus_tag="VN496_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXF17105.1"
                     /translation="MIGRRRAVRWSTPGVAFALLCACAQEPHHSPGRNVSLVVLPKAP
                     DGHVGAVMMHPAGGGKPVLIDKAYVQASMRDSKTVQTAPVDPKQVSESFGRTLDALPA
                     LPTTHLVYFVEGTEELKPEAKRAIERVFAEIQTRPAPEISVVGHTDFVGTDQYNDTLS
                     LQ"
BASE COUNT         3146 a         3658 c         3885 g         2808 t
ORIGIN      
        1 tttgggcacg cctatcgcga gactcggccg actcgcagtg gcaccggacg gcgcagtgtg
       61 gtttgctgaa agcaccacgt acggcttcac gcggttaaag gacggcgtac taacccgcaa
      121 tgtaccaaaa tcggtgcgcg gcggtccgta tggcgtagcg gtcgattcga aaggcgacgt
      181 gtggggcacc ctgcaaagcg gcaaccaatt ggtgaaaatc tctcaaggca aagacattgc
      241 cgaatacgaa cttccaacgc ccggaagttc gcccagtgac gtcgcggcag acagtgccgg
      301 aaacgtctgg ctactcgaat tccgtagcaa caagattgca aaattcagca acggaaaatt
      361 ttcggagttc gttgtacccg gtgaatggtc cggcctgtct ggaatcgcca ccgcaacgga
      421 tgggtcggtc tggtttggct tgctgcgcaa tcatgccatc ggccgccttc gcaatggcga
      481 attcaagatt tttgagctac ctcgcaaaga cgctcgtccc tacacgctgg caattgataa
      541 ggattcgaac gtctggtacg cggacatcag cggttatgtc gggatgctaa aagcggatgc
      601 ggcaactcga tagcagagga atttggcgcg tggctcgtac acgccggacc ttgcctgggc
      661 tgaatatccg ttaagtcagc gtactgagcg tacgccaggc tccgaaagag agaatcgttc
      721 cgacaataac aatcaacagc gaagttccca cctgcagcct catggcccat ggacccgcga
      781 gccagtcgag aatcctttga ccgactttag cggcgaccac gccaacgact accaacgtac
      841 cggcaaatcc gatactgaac accaccacgg tacctagccc gagcatgact ttgccttggg
      901 caagcgcatt caacaagatc gcaagcgcgg ccgggtccgg cagtaagcct ccggcaactc
      961 caagcccaat aagtatccag agcccgggcc ggttggttgt tgcgagcttc atatcatgcg
     1021 tgtgcttgaa tccccagcca tggctgtgat atcccgcttc ttcatcgtgc tggtgatctc
     1081 cactatgtgt atgtgtgtgg gccgcaccgc gatgggaatg ttgaccgtgg tgatgtgagt
     1141 gactgaattg atgctcctgg ctgtgcgtat gtggccgaaa ctgcattggc gcatgattga
     1201 tacccgcagg atgcggctgg agttgctcgg ttgcactatc tgtatgcaac aacccggaag
     1261 aagttgtgcg cgggacgcct tgtaacagca tgatcaggtc tctctgtgtc cacagtgtcc
     1321 atagaccaat aattacgacc aacaagcccg tagcgagcgc cagccatgcc tcgatccgtt
     1381 gcggcaccat tcccggcatg ccgatcgatg cgaggatgcc gaccagcacg atgccactcg
     1441 tgtgtgaaag agtaacgaag attccgagga tgacggcgtc gaccggttta ccgcgggcgc
     1501 caacaatata tgccgccgcg atagtcttgc catgtcccgg agtagcggcg tggacggcac
     1561 cgatccaaaa cgcagaagcg aggtatatca gtgcatcggt catcggagat aatcggagag
     1621 gctcgttatt gtgaggggcg ggcgcagaat aggtgccgtg tcgggtgctg tcaagcaggc
     1681 gggcggcccc agcccagtgc gagctacaca gagcttcgct tcagcagcag ctgatcggat
     1741 tggatgcgat tatcaatcgc gcgatttcat tatgtccatg ttgaatgtgc cgacgagaca
     1801 gaagtggtcg gaaagctggg cacaaggtcc gcgcacggtc aagacaggta cgatccttca
     1861 gatcgccaga tattttttca caattttggg ttcgagttcg atgcccatgc ccggccgctc
     1921 cgacagcgta atgttgccat tcttgtccac caacagcggt tcttttacca actcgttcat
     1981 caatgcatgc gcggggaaca cagtctccgc cattgagcca ttgcgaaccg cgccgagcag
     2041 gtgcactgcc gctgcaaccg ctactgcgga accgtggatg tgaggcgcga tcggtagatt
     2101 tgccgcctgc gccatgtgag caatcttcag cgtttccgta aggccgccgg cccaggcaac
     2161 atcgggctgc acaaagtgga ctgcacgcgc tttgatgtaa tcattgaaat ctacaatacc
     2221 gtagctgcat gtttcgtatc cggccagcgc tatgtcaact ttcgctgcga cttccacgct
     2281 gccagcaaca tcatccggcg acactggctc ttcgtaccaa tagatgtcgt aaccttgcat
     2341 ggcgcggccc atccttactg cggtcggtac atcccaggct gcattagcgt caaccatcag
     2401 tttgatgtcc ggaccaatgg cttcacgcac tgctgccacg cgcgcaatat catcggcaag
     2461 cgcgaccagg cattcgctgg cattgtccat cgcacgcaac ggtgaaagtt cgatgcccgg
     2521 attgcgtccg accttcatct tcacggcgcg aaaaccatgc ttgacgtaag tttccatctc
     2581 cagagccagc tctttgatgc ccttgccgac tgaatagaag ccgcctgtag catatgcggg
     2641 tacggtttgg cggtaaccac ccaacaatcg ccaaagcggc atgccggcca ttttgcctcg
     2701 caggtcccac aaggcgatat cgatgccgct gattgcggca atcactattc cgccacgcgc
     2761 atgcttgtac gcacgacggt gcattacctc ccacagatat tcgatattgc gcgggtcttg
     2821 tccgatcaga taatcacgca attcattttc gatgacttgc gacgtggatt gcatcggtcc
     2881 gccaaaatgt gcagcctctc ccaaaccaac aagcccctga tcggtctcca cctcgacaag
     2941 cagggctggt tttgtcgcca tcgtatagtt tgcgtcatag actggttttg gaagtttgac
     3001 ttctaccggg atggtgcgga catcggtgat acgcataaaa tcaaatcctt tggcaaaatt
     3061 tttacgcgag aagattttct ctgtaatccg ttgctgaaaa attaagcagc actaacgagc
     3121 gcatcagttc taatactgac atacggcaaa tgtttcacgc catccacaag gttatccaca
     3181 gaatctgtgg atataaattc caggaaatct tttcctgtaa ttcgggcgtt atttccttcc
     3241 tgcgacaaaa atgtcgtgat cgggcgacat tcgcaattga tccggacaaa tttcgcgttc
     3301 gcgagattgc ttcagtctgg aggccgtgca gacacatcat ggttggtttt gcccgttagc
     3361 gttatttgca ccagccattc gtcgtcgtgc ctgcgataga tctgcacggc gtcttcacgc
     3421 caacacgact ttctggcgtc ccatgcgcct gcgcgcaatt ttctaaactt ctttccacta
     3481 aatgcccagc gaagcaacgc gacgaagtgc ccgctaactc cggttaagca atacgcgaat
     3541 acctggccga acatcgttca gaccattacg ctagcgcgct taccgagcca acggacaatc
     3601 agatgccaaa gttcgccgcc aatttacatt acttgtttac cgagctgcct tttctccagc
     3661 gttttgaagc cgcggcgaac gccggatttg aagcagttga gtttcaggtt ccgtatgact
     3721 atccggaaac tgaattatcg gcacgcttga agggcaacaa actgcaaatg gtgttgtttg
     3781 atgcgccgat gggagattgg gcgcggggag accgtggtct ggctgcagta gccggcaggg
     3841 aaagcgagtt ccgtgcgtcc ttggcacgcg taattgaata tggcaatgcg ctgaactgcg
     3901 ataccgtaca tttgatggct ggtgtaatcg gtgccagtga agacttgcac aacgcagaac
     3961 acacctatat tgcaaatcta aggcacgccg ctccattatt gaaggagcat ggcattacgg
     4021 ccgtcattga acccattaac aagaaaatgg gaatcagcaa ggatgcgccc agctatacga
     4081 cgcatggcat gcatggctat tttctgaacc acaccgagca ggcgcggcgc attatccagg
     4141 cagttggcag cccgaacctg ctgctgcatc tcgactgtta tcacatgcag atgctggagg
     4201 gccatctatc ggaaactttt cgagacaaca ttgccctgtt gcggcatgtt caggtagccg
     4261 gcgtcccggg ccgccatgaa ccgacggttg gtgagattaa ctatccttat ctgtttaatt
     4321 tactggacga actgagctat cgcggctgga tcgggtgcga atatcggcca aagggcgata
     4381 cctggtccgg cctttcctgg gcatccgctt atggcatacg ggctgaccgg tcagataacc
     4441 ataagggacg agccgggtag gcaaaaaaag aggggcgctt ttcgcgcccc tcgaaccacg
     4501 taattcaatt tgaggagaga gttacgtggg ggaggaccgt tacccaccgc ccctcagggc
     4561 aggtagggaa atgtgcttgc atcctggtta gagagatccg gcaactgctc ttcgctgact
     4621 ttctgcgccg tcgcgctcga gacggcaggc ggcggaccgt cgaattcata caagcgcagc
     4681 aacgaatcga atagcgattt catgtttggc tccgtcaaag gcataagcga cgaaaccact
     4741 gtaaccttat gcctagaatc ctactagctg tcaaaaatga ataggattta tgcaatttta
     4801 gtgataagtt tgcggcatgg atcgattata tcaaatgcgc ttgttcgttc gcattgtaga
     4861 aaccggcagt ttttcagccg tggcgcgcga actcggaacg attcaaccca ccgtcagcaa
     4921 acaacttact gctttggaag agcatttagg cgttcgtctg ctaaatcgga cgacgcggca
     4981 actcagtacg acggaagctg gccgcaagta ctacgaccga tgccggcaca tcctggacga
     5041 aatcatcgat ctggaaggca gcctgaccga gctccagaac agcccaaccg gcttgcttcg
     5101 cattaacgca cccgtcgctt ttggccagct ttacatgttg cctctcgtat tccggttccg
     5161 acggctgttt ccgggactcg cgattgatct gacgttaaac gatcgttacg tcgatctggt
     5221 tcaggaaggc attgatgtgg ccattcgctt cggcgagtta tcagattctc aagtggtcgc
     5281 aagaagtgtc ggaagagtga cgcgagtttg cctggctagc ccggcatacc tgcgggccag
     5341 gggagttccc aaaacgccca acgacttgct gtcgcacaac tgcattacct ataactactt
     5401 atttactaac gagtggcaat ttggcggacc ggaaggtccg acgactctgc gtgtggccgg
     5461 ggacttccgg gcgaatagcg ccttgaccat ccgcacagcg gcactggaag gaatcggtat
     5521 cgcaaacatg cccagcgtgt tcgtgcaaaa cgatctggat aatgggagcc tgatccgggt
     5581 gctcgccgac ttcgcggcgc caccagttga aattcacgcg ctctatccct ctgcgcgctt
     5641 tttatccagc aaggtcaggc tgtttttgga tttcgtacgc gatgaattcc ttcagatagc
     5701 gtcactccag gttgctgcga taaaacccaa acgaatcccg cgcatccgcg cgaaagtgac
     5761 aggttgatct cacggcgctt agaatacgcg ccgctacctg tacctgtagt cgatagcgtt
     5821 cgcaacgagt tgcagatctt tcccggcccc ctgcttatcg caccaaagga cctaatgcca
     5881 aagaagccaa acgcctacac cgctgaagac atcgagattc tcgaggatct gtctcctatc
     5941 ctgcatcggc cgggcatgta taccgatccg gccagtccga atcacgctct ggtcgaaatc
     6001 atagataacg ctgcggatga gggtttggcg ggtttcgcca aaagtgtgga ggtgacgctc
     6061 tacgaggacg gttcggcaca agtcactgac gacgggcgcg gtattccggt cgatatccat
     6121 ccaaagaaaa aggtgcctgc tgtccaggtc attttcacca cgttgcattc aggcggtaaa
     6181 ttccgcaaga cggataaaga tgccgcgtac cgcatcgccg gaggtctcca tggcgtgggc
     6241 gtttgcgtgc ccaatgcgct ttccacgagg ctggaagttg aagtaaaacg cgatggtgaa
     6301 aaaagtcgca ttgtgttcgc cgacaacggc aaagtgaaag aacccctgag gaagaccgct
     6361 acaatcggtc cgcgcgatag cggtactcgc gttcgcttct ggcctgaccc aaaatatttt
     6421 gactcgccca aaattgtcgc agcagagatc gcggcgctgt tgcgcgcaaa ggccatgctt
     6481 ttgccgggcg taaaattttc ccttgcgacc gaacgaaaca aaaaattcga agtacagacc
     6541 tggcactttc ctgaaggcat caaaggctac tttgcttcag ccatgcaggg gcaggagccg
     6601 gttgctgaaa cttttctcgg cgagcgctac gtggcggcgc aatccgagtc ggacaatttt
     6661 gcagaggggg aaggcgcgat gtgggcgatt gcgtggaccc ctgagggtga gccggtcgcg
     6721 gaatcttatg tgaacatgat tccaacgcgg atcggcggta cgcacgtcgc tggattgcgt
     6781 gaagcggtct acaacgcgat caagaacttt atcgatttgc acgcaatggg acagcgagga
     6841 ttgaaaattg tgcctgagga tgtctggtcg cggacttcat ttgtactggc aatcaaaatg
     6901 ctggatcccc agttcaaggg gcaggtaaag aacgagttga tttcacgcga cgcggtcaag
     6961 ctcactgccc agatggtgcg tgatcctttc gagttgtggc tgaaccagca tgtagacgaa
     7021 ggcaagcgca ttgcggaact tgtcatgagt caagccaacg cgcgcactcg ggccgcgcag
     7081 aaaattgaac gacgtaaact gtctggcgtc gcggttctgc caggcaagct gaccgactgt
     7141 gcctcggagg atccggagcg aaacgagttg ttcattgtgg aaggtgattc cgccggcggt
     7201 tctgccaagc aggcacggga caaagagttc caggccgtgc tgcctctcaa aggcaagcct
     7261 aagaatacct ggcaggatag tgccgatacg ctgtacgcca ataaagaaat cgaagctatc
     7321 gcgctcgcga tcggcgtcga ccatcacaag tcgacggata ctgtcgatct ggcgggcttg
     7381 cgttaccgca aagtcatcgt gctggccgat gcggacgtgg acggtgcgca tattcaggtt
     7441 ttgctgctga cgctgttctt caggcatttt ccgaaactga ttgaaggggg caacatttac
     7501 gtggcgcaac cgccactgtt ccgcatagac gtatcctcgc aaggcaaagg caagccggcg
     7561 cgaaaacttt atgcgctgga caaacaggaa ctcgatggca tcgaggaaaa actcgaagat
     7621 gagggtttca agcccaattc ctggactgtc agccgcttca agggacttgg cgagatgaat
     7681 cccgaacaac tttgggaaac caccctcaat cccgatacgc gccggatgct accggtcaga
     7741 gtcgacgacg gcgccaacat atcagtggac atcttcaaca tgatgatgtc gcgcgaaaat
     7801 gcttcgcagc gccgcgaatg gatggagacg tacggcaacc tggttgaagc ggacatgtca
     7861 tgacaaatgc ctgcaacacg ttgctgacat gaattcagcc tcaaggcaaa cggaattgtc
     7921 gcaataacga acaacgacac atggcggatc aacctgactt attcggcaac ggtgagacga
     7981 ccgttctcga caagaaagta aagaccaagg agctggccgt cagcgccggt ggggccagcg
     8041 gtggcggact gccaccgcat ttcgcaccga tgtcggggga tcttggagat tccctgccgc
     8101 tcggcaaata cgcgtcgacc caatacctgc agtacgcgat cgcgacggta aaagaccgcg
     8161 cattaccgcg cgtaggtgac ggccagaaac cggtgcaggg gcgaattctc tattcaatgt
     8221 ggaccaacaa taatcgctcg gggaccaaac gcaccaagtc ggccgcggtc gtgggtgaag
     8281 tgctcggaaa actgcacccg catggcgatc agtcggtgta cgacgcgctg gtacgtctgg
     8341 cgcaggattt caccatgcgt tatcccctga tagacggcga gggtaacttt ggttcgcgcg
     8401 acggggatga ggcggcggct taccggtaca cggaggcgcg gctgaccaaa ttcgcggaag
     8461 tgttgctcgc ggaaatagac atgggcacgg tggatttcag ccaaaactat gatggcaccc
     8521 gagaagaacc cacgattttg cccgctcggc tgccggtcct gttgctcaat ggtgcttccg
     8581 gtattgcggt tggcatggcg acggaaatcc cgagccataa tctcaatgaa gtggctgctg
     8641 ccgccatcgc gctgatccgc gatccggagc tgaatatcac cggcctgatg aagcacatca
     8701 agggaccgga ttttcccgcc ggtgcgcaga tcattacgcc gagggcggag ctgaaggagg
     8761 cctacacgac ggggcgcggt gcagtgcgtg tgcgagcgcg ctggaatatc gagcgcctcg
     8821 cgcggggaca gtggcagatg gtggtgtacg agctgcctcc cggtgcgtcc gcgaaaaaag
     8881 tgcttgaaga aattgatggg atcaccaatc cgcagagcaa gccaggcaaa aaatcgctga
     8941 cgcccgagca aacccgggaa aagcagttga tgctctccat gctggacaaa gcccgcgacg
     9001 aatctgaccg gacgcatccc gtcagactgg tgttcgaacc gaaaacatcg aggattgacg
     9061 agacggagtt cgtcaatctg ctcctggcca agacgggcat ggagggcaat gtcccgatca
     9121 atctggtcat ggtgggactc gatggccggc ccacgggcaa gaacttgcgc gacatcctgt
     9181 cggagtggat ccggttccgt ttcacgaccg tcacgcgtcg tacgcgccat cggcttacgc
     9241 aggtgctcga ccggatccat gtgcttgaag ggcgcatgat cgtactgctc aacgtagata
     9301 aagccatcag gatcatccgc aatgcggacg aaccaaagcc ggaactgatc aaggcattca
     9361 aactgtccga gcgtcaggca gaagacattc tggacatacg tttgcgacag ctggctaagc
     9421 ttgaacagat caggatagaa caggagttga aggagctgaa aaaggaacag agaagcctgg
     9481 aaaaaatact gaaagacccg aaggtgctgg gcgaactggt gacttcggag attgaggccg
     9541 acgtcaagac attcggagac aagcgccgta cccgaatcga ggaatccgag aaagcggcaa
     9601 tcgaaacgcc aattatcgat gaaccggtta ccgtgatttt ttccacgaaa ggctgggtgc
     9661 gagcccgtca gggatgggaa gtcgaagccg catcgctgtc gttcaaggaa ggcgacggcc
     9721 tcatgtcatt gatcaaatgc cgcacgatcg acccggtgat tttcatggat tccaggggca
     9781 ggacctatac ggtagaagcg ggccaactgc cgcctgcgcg cggcgatggc gcaccggcgt
     9841 cttcactggt ggaagtacag gacggagcac acattctgta ttgcctcagc ggcaagcccg
     9901 ataccaaagt gctggtcgct tcgacggctg gctacggctt tctgtcgagc gtaggggaca
     9961 tgatctccag ccggaaggcg ggccgcgagt tcatggcggt tgatgacggc cacactccct
    10021 tgccgccata tgtttatcag gagcagccag gcaattatgt cgcctcgtta tcggagggtg
    10081 gacgcttgct gctgttcgcc gtcgatgagt tgaagatggt ggcaagagga cgcggcatga
    10141 ttgtcatggg cttggatgat ggcgaaaaac tggccgctgt agcggtttca aacaagcaga
    10201 ccctgatggt gacaggtatc ggtcaacgca ctgcgaagga aaagacaatc ctgctctccg
    10261 gccagaaact ggctcaccat atcctccggc gcgcgcgcat ggggcgggtg cttccggaaa
    10321 aactcaaaat gcctttgcga atcgcggtag agtaagcgtc cggttatggc gactttgtat
    10381 ccggtagagg ggcgcaatgc gcccctttgc cttgccgagc ccgcatcttc aatcgcaagc
    10441 gcatttgatt cggaggcgta atgaaagcag acaacattct tgggacgatc ggaaatactc
    10501 cacacattcg catgaaccgt ctgttcggca cggggcagaa tgtctggatc aaatccgaac
    10561 gtgccaaccc aggcggctcc atcaaggacc gcattgccct gtccatggtg gaggatgccg
    10621 aaaagcgcgg caagctcaag cccggcggga cgattattga gcccacctcc ggcaatacag
    10681 gtattggact ggcgctcgta ggcgccgtaa agggttatgc agtcatcctg gttatgccgg
    10741 acagcatgtc ggtcgagcgg cgtcgcctca tgctcgcgta cggcgccaaa ttcgagctca
    10801 ctccacgcga aaagggaatc agtgcttcga tcgcgcgcgc gcaggaactg cttgcggaaa
    10861 tcccaggcgc ctggatgcca cagcagttcg agaatgcggc gaacaccgaa gtgcatgcac
    10921 gcactactgc gagggaaatt ctcgccgact ttccggaagg catggatctg ctgatcaccg
    10981 gcgtcggaac cggaggacat atcaccggct gcgcacaggt gctcaaaaaa gcgtggccca
    11041 agctcaaggt gttcgccgtc gaatcggctc tctctcaggt catcgcgggc gggaagcact
    11101 cgcctcatcc tttgcagggc atcggcactg gattcatccc gaagatcctg gacactgctt
    11161 tgctggacgg gactattccg gtggaagcag aggccgccaa ggaaatggcg cgccgcgcgg
    11221 cgcgcgaaga aggcatgctg gtcggacttt cttcaggggc caccttggcc gctattgcgc
    11281 agaagctgcc cgaatttcca aaaggcacga gggtaatcgg tttcaattac gacactggcg
    11341 aacgctatct ctcggtcgag ggcttcttgc caacaggagc atgactttcc ttattaagaa
    11401 tcgcgagcgt tgcagcgagg gatcggttcc cgggcatgtt tggccggaca ctaagcagat
    11461 taactctgca taataggtac tgaacgctgc ccgggtcgaa gcggcgtcgg tttcaaaaag
    11521 gggataaaac atgcaaccgg tactggcctg gcttcttgca ggtctgctgc ttgtcgtcgt
    11581 cgagttgatg acggggacgt tctatctgtt gattctgggt attgctgcag gaatcggatc
    11641 ggtaattgcg ttcctcgaac agcctttctg gatgcaagct ttggctgccg ccatttcggc
    11701 aatcgtcggc ggcgtgctgg tgaatcgtta ccatcgtgcg gcgaacgcca gttcacccag
    11761 ggacagctca aatgacatcg gagaacccgt aagcatcgaa agctgggtca gtgagcccaa
    11821 ccggctggca cgtgttcgtt atcgtggaac cacctgggat gcggaggtca ttggcaccga
    11881 gcggatcgaa gcggatgcac tgctttatgt cgtggcgacc gatggcagtc gcctgaaaat
    11941 atcttcgtcc cggccgccag agcatcacca caaacataaa taggagagcc gtcatgcttc
    12001 ccaaacttat tgtcgccctg gttctctcgg ccatgatctt catgtatccg ccgacgcacg
    12061 gctacgccgt gccgttcttc gtcattgcgc ttgccgtggt gttcgtcatc gagggcgtga
    12121 aggttgtgcc gcagcagaat gcgtgggtgg tcgaaaggct cggcaagttc aacgcgatcc
    12181 tggagccggg cctcaacttc atcatgccgt tcgtcgaccg cgttgagtac cggcattcac
    12241 tcaaggaagt gccgctcgat gtgccggagc aggtctgcat caccaaggat aatacgcagt
    12301 tgtccgtgga tggcatcatc tatttccagg tcacggatcc gcgcctggca agctatggca
    12361 catcgaacta tgtattcgcc attacgcaac ttgcacagac cacactgcgc agcgaagtgg
    12421 gcaagatgga gctcgataaa actttcgaaa gccgtgacga aatgaacaag caggtggttt
    12481 ccgtgcttga cgaagcgggc cgcacctggg gagtcaaggt actgcgctac gaaatcaaga
    12541 atctcacccc accggaagcc atcctgcgct ccatgcaggc gcagatcacc gcagagcggg
    12601 agaagcgtgc gctcatagcg aaatcagaag ggcaacggca gcaggaaata aatctggccg
    12661 atggcgaaaa gcaggccaaa attctctcgt cagaaggcga caagacggcg cagatcaaca
    12721 aggctcaagg cgaggccggc gcaatcaaaa tgattgccga agcgcaggcc gaagcggtgc
    12781 gggtcgtcgg acaagcaatc ggtactgctg aaggactcag cgcggccaat ttgaaagtcg
    12841 cggagctcta cgtcggcgcc ttcaaggaac tcgcaaaagc caacaacacg ctgatcattc
    12901 catccaactt gagcgacgcg gccagtttcg tcgcgagcgc gatgaccgtg ctggaccggg
    12961 caaagctggg cacggagacc caaaaggccg cgcaggccta ggatgtaaac atgatcggca
    13021 ggcgacgagc cgtgcgctgg agcactcctg gtgtggcttt cgcgctgcta tgcgcgtgcg
    13081 cacaggagcc gcatcactca ccagggcgga atgtctcact ggtggttttg ccgaaggctc
    13141 cggatggtca cgtcggcgcc gtgatgatgc atccggccgg cggcggcaag ccggtgttga
    13201 tcgacaaagc ctatgttcag gccagcatgc gggatagcaa aacagtacag acagcgcccg
    13261 ttgacccgaa gcaggtcagt gagtcgttcg gcaggactct cgacgccttg cccgcactgc
    13321 cgacgacgca tctggtttat ttcgtggagg gcacggaaga attgaaaccg gaagccaagc
    13381 gggcgatcga gcgcgttttc gcggagatac aaacgcgccc ggcgccggaa atttccgtgg
    13441 tggggcatac cgactttgtt ggcaccgacc aatacaacga tactctgtcg ctccagc
//
LOCUS       DATMZQ010000272         6165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2639872, whole genome shotgun sequence.
ACCESSION   DATMZQ010000272 DATMZQ010000000
VERSION     DATMZQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2639872"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VN496_08880"
     CDS             <1..516
                     /locus_tag="VN496_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXF17106.1"
                     /translation="DSARESVFAAIEASKGAGGAFVFDCNLRLRLWASLAHARKVITE
                     TVSKADYFLLSMEDAEALCDLHDPLGIIAWCHRAGARTVALKLGPGGVIGSDGARHEH
                     IAGYKVNSVDATGAGDCFAGALLARMSAGEGFWEALVYANAAAALTTTGYGAVAPMPR
                     PEAVRQLLRGG"
     gene            complement(554..733)
                     /locus_tag="VN496_08885"
     CDS             complement(554..733)
                     /locus_tag="VN496_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17107.1"
                     /translation="MTAVAAACYDSHVVAKVSCTIRAPQCAAIEGDAIMSMLDIALVA
                     GVLLLAVLIVARKKR"
     gene            complement(1000..1305)
                     /locus_tag="VN496_08890"
     CDS             complement(1000..1305)
                     /locus_tag="VN496_08890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXF17108.1"
                     /translation="MARYVITVDFYLHAGTLEPFLRLIKENARQSVADEPGCHRFDVL
                     IEKGAPDHIVLYEIYQDRAAFDLHVKSRHFAQFNAASNRYVREKKIVEYEYTNDDRD"
     gene            1610..2119
                     /locus_tag="VN496_08895"
     CDS             1610..2119
                     /locus_tag="VN496_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HXF17109.1"
                     /translation="MSRAFVKEENEALTDEALPERPVSAQPNYVTPAGLEQLRAKVDA
                     LHTEHSRLKRAAEDFDRPKLSQIERDLRYYQAKLDTAILVNIAAQPRDEVRFGASVQT
                     EDENGGLHAFTLVGEDEADVAQGKVSWHSPLAKALMGAKIGDTVTWNRPAGAMTLEVL
                     DVRYAEKPA"
     gene            2173..3489
                     /locus_tag="VN496_08900"
     CDS             2173..3489
                     /locus_tag="VN496_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXF17110.1"
                     /translation="MKQFSRRAVLKALGASALSAAAGLPGAPAQAAPLSYKPEADAQL
                     RVLRWKRFVQGDEDQWMANTQKFTQATGVPVRVDSENFEDIRPKAAVAANIGNGPDIV
                     MGWYDDPHQYADKLLDLSELATYLDGKYGGWYEVCKRYCIHEGRWIAIGAGFLGGCLV
                     YRQSMLEAAGHEKPPASTDGFLKLCQALKAKGTPVGFALGNAVGDSNAWTHWALWAFG
                     GRVADEGNQVVINSRETGAALEYAKALYQTFIPGTLSWLDPSNNKAFLAGDISLTLNG
                     VSVYYVAKNSPEPALKKMAQDIHHVNMPIGPSGKPAELNPFTPMFVFHYTKYPNAARE
                     YLRFMMEREQYEAWQSSSLGYVQQPLKAYAETKFWKTDPKLLPYRNVPALTRDNGYAG
                     KLGAASAGAMADYIVVNMFAQAASGASSIEAAMKLAEGRAKRYYRG"
     gene            3650..5329
                     /locus_tag="VN496_08905"
     CDS             3650..5329
                     /locus_tag="VN496_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF17111.1"
                     /translation="MNDLIIDNATIYDGSGTLPQRGSVAIQGDRITAVGQCEGAARNR
                     IDANGLALMPGIIDTHTHYDAQITWDPFASPSPALGVTTVIMGNCGFTIAPCRPADRD
                     LVMRNLTHVEGMSLDALRTGTDWDFESFPQYLDMLERKGVGPNAAVFVGHSSVRTWVM
                     REGATKRTATDDEIAQMRSLIKEAMQAGAVGFSTTRSGQHNGEAGVPMPSRLADEKEL
                     MALSTALKDAGHGVLMMTKDARATTMSSIEAIARASGRPYVVAALLHSNETPEATFED
                     LEAIKQARGRDAKLYGAVSPCPLNFEFTMHEPYVFEGLAAWRPVMEAAKEDVPRILAS
                     KALRDNVKKELAVRSRRMFNGHWHQMFVAQTARPENRHMESHSVQELAQQAGKHPFDF
                     ALDLALEENLDTLFTATLLNSDDDAVGRMMLDPASMISLSDAGAHLTFLCDAGFGLHL
                     LGHWVRSRKLMPLQEAIRRITSEPAQIFGIKQRGTIKPGMHADLLLFDPDTVGRKRAE
                     RVYDLPAGASRLTTPATGVHGVWVNGKQVADAHGIRADAPKAGKVLRGFAA"
     gene            5655..6044
                     /locus_tag="VN496_08910"
     CDS             5655..6044
                     /locus_tag="VN496_08910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF17112.1"
                     /translation="MTGTVVVLPAKVQIQGTCSDEFTPVRAAFTHNLETGQDVGASVA
                     VFVEGQCVVYLWGGYLDGTYTRPFERHTIVQGYSLTKTVTALCALVLADRGEIDSDVP
                     MAKYWPESAAEGKRDIQMGQLLGHSRR"
BASE COUNT         1279 a         1773 c         1923 g         1190 t
ORIGIN      
        1 gacagcgcac gcgagtcggt attcgcggca atcgaagcct ccaaaggcgc gggcggcgcg
       61 tttgtattcg actgcaatct gcggcttcgg ctgtgggcgt cattggccca cgcgcggaaa
      121 gtcattaccg agaccgtgtc aaaggcggat tattttctgc tcagcatgga agacgccgaa
      181 gcgctgtgtg atttgcatga tccgcttggc atcatcgcct ggtgccatcg cgcgggtgcg
      241 cgcacggtgg cgctaaaact cggtccgggc ggggtaatcg gcagcgatgg agcgcggcac
      301 gaacacattg cggggtataa ggtaaacagc gtggatgcga ctggcgcggg cgattgtttt
      361 gcgggggcat tgctggcccg catgtcggca ggggaaggct tctgggaggc attggtctat
      421 gcgaacgcgg cagctgccct gactacaacg ggatacggtg ccgtagcgcc catgcccaga
      481 cccgaggcgg tgcgtcaatt gctaaggggc ggatgacact tcccgggtgg ccccgggagc
      541 gccaaggcgt tgattagcgc ttcttgcgtg cgacgatcag tacagccagc agcaacacgc
      601 ccgcaacgag cgctatgtcc agcattgaca ttattgcgtc tccctctatt gccgcgcact
      661 gcggtgcgcg gatcgtgcat gaaactttcg cgactacatg actatcgtag caagccgctg
      721 caaccgccgt cacgtcgttt ctttcgattg cccggagaag tctaggctta tgtaaaaagc
      781 cgtgtgcctg aacgcaatgc ttttgaagcg gttataccga cttcggtaac cggtgcggat
      841 tgcaccatat agctcttcgc ggcgtttatg gttgcccgga gagaccgcgg agatatgggc
      901 agtagccctc tgcttactcg gacatttttg accgacggta ccaggccggc ggggtgcggc
      961 aattaggatt tgttacggca gttacggtta tttctgactc taatcgcgat catcgtttgt
     1021 gtattcgtat tccacaatct ttttttccct gacgtagcgg ttcgatgccg cattgaattg
     1081 cgcaaaatgg cgcgacttca cgtgcaggtc gaaggcggca cggtcttggt atatctcgta
     1141 gagcacgata tggtccggcg cccctttttc gatcaggaca tcaaaccgat gacaacctgg
     1201 ttcgtctgcg actgactggc gggcgttttc cttgatcagc cgtagaaacg gttcgagcgt
     1261 gccggcatgc agataaaagt caacggtgat gacgtagcga gccattggca tcaagcaatg
     1321 cgtgatttgc gaacagggaa catccttaca gcataaaacc ttacttgcaa ccgccgaccc
     1381 gcgcaagtcc cgcgccagaa cttgcgcatg cgcgcccgcc attgccggcg accgcgcaat
     1441 cgaatctatg gcggggtctc cgccgccgca tattgtttgc ggaacattgt cgcaacaggg
     1501 tgttcaaact cgcgtgggac agtacgggcc gtactcgtgg tgtcctccat ccctttgccg
     1561 ccgtggcctg cggaggcggt caatatccga atgcgatgag gtggaccaga tgagccgtgc
     1621 tttcgtcaag gaagaaaacg aagcgctgac agacgaagcc ctgccggaac gcccggtcag
     1681 cgcgcagccg aattacgtga cgcctgccgg gctcgaacag ttgcgcgcga aagttgatgc
     1741 actgcacacg gagcacagcc gactgaagcg cgcggcggag gatttcgacc ggcccaaact
     1801 gtcccagatt gaacgcgatc tgcgctatta ccaggccaaa cttgataccg ccatcctggt
     1861 caatatcgcg gcgcagccgc gtgatgaggt tcgtttcggc gcgagcgtac aaacggaaga
     1921 tgaaaacggc ggcctgcacg cgttcacgct ggtaggggag gacgaggcag acgttgccca
     1981 aggcaaggtc agctggcatt cgccgcttgc caaagcgctc atgggtgcaa agattgggga
     2041 cacagtgacc tggaaccggc cagccggggc aatgacgctc gaggttctcg atgtgcgata
     2101 tgccgagaaa cccgcgtaga cggttagcgt ggtttaggcg ttttcgtccc ttgtcctgca
     2161 agcgaggtca ccatgaagca attttccagg cgtgcggtgc taaaagcact cggcgcgtca
     2221 gcgctttcgg cagccgccgg cctgccaggt gcgccggcgc aagcggcccc actcagttac
     2281 aagcctgaag cggatgccca gctgcgcgtg ctgcgctgga agcggttcgt tcaaggcgat
     2341 gaagaccaat ggatggccaa cacgcaaaag ttcacgcagg ccaccggagt cccggtgcgg
     2401 gtggattcag aaaatttcga agacatccgg ccgaaagccg ccgtggcggc caacattggt
     2461 aacggaccgg acatcgtcat gggttggtac gacgatcccc atcaatacgc ggacaaactg
     2521 ctcgatttga gcgagttggc cacgtatctg gacggcaagt acggtggctg gtacgaggtg
     2581 tgcaagcgct actgcatcca cgagggccgc tggatcgcga tcggcgctgg ctttctgggc
     2641 ggttgcctgg tctaccgtca aagcatgctt gaagcggccg gccatgaaaa accgccggca
     2701 agcacggatg gttttctcaa gctgtgccag gccttgaagg cgaagggcac gccggtcgga
     2761 ttcgcgcttg gcaacgctgt gggagactcc aacgcgtgga cgcactgggc gctatgggcg
     2821 ttcggggggc gcgtggcgga cgaaggcaac caggtcgtca tcaattcgcg cgagaccggg
     2881 gcagcgctgg aatacgccaa agcgctctac cagactttta ttcccggcac cttgtcctgg
     2941 ctcgacccga gcaacaacaa ggctttcctg gccggggaca tcagccttac gctgaacggc
     3001 gtgtcggtgt attacgtcgc gaagaattcg ccggagccgg cgttgaagaa aatggcgcag
     3061 gacattcacc acgtaaacat gcccattggc ccgtccggga agccggccga gctgaacccc
     3121 tttacgccga tgttcgtatt ccactacacc aagtatccga atgccgcccg ggagtacctg
     3181 cggttcatga tggaacgtga gcagtacgag gcctggcagt cgtcctcgct gggttatgtc
     3241 caacagccgc tcaaggccta tgcggagaca aagttctgga agaccgatcc gaagctactg
     3301 ccgtaccgga atgtgccggc actgacccgt gacaacggtt acgccggcaa actgggtgcc
     3361 gcttccgctg gcgcgatggc cgactatatt gttgtaaaca tgttcgcgca ggcggcatcg
     3421 ggcgcctcca gtatcgaagc tgcgatgaaa ctggcggaag gccgggccaa acgctattac
     3481 cgcggttagt tcagagcggc cgcgctgcac actgcgtcgg ccactgcgtt cctgaaattc
     3541 gtgctgccgc aacagccgga cgcacgcctc cggtaaactt ctctcccaag gcgccggtga
     3601 ccggagccac atcaatgaag tccgcgctat gcggctccgg gagaaggcaa tgaacgacct
     3661 gatcatcgac aacgcgacca tctatgacgg gtcgggcacg ttgccgcagc gcggcagtgt
     3721 cgcgatccag ggggatcgca ttaccgcagt agggcagtgc gaaggcgcgg cgcgcaaccg
     3781 tatcgatgcc aacggcctgg cactgatgcc cggcatcatc gatacgcata cccactatga
     3841 cgcgcaaatt acctgggatc ccttcgcgag tccgtctccg gcgcttggcg tcaccacggt
     3901 gatcatgggc aactgcggct tcacgatcgc gccttgccgc ccggcggatc gcgacctggt
     3961 catgcgcaat ctgacccacg tcgagggcat gtcgctcgat gcattgcgta ccggcactga
     4021 ctgggatttc gaatcgtttc cgcaatatct ggacatgctt gaacgcaaag gcgtcggccc
     4081 gaacgcagcc gtattcgtcg ggcattcttc cgtgcgcacc tgggtcatgc gcgagggcgc
     4141 aaccaagcgc acggcaacgg acgacgaaat tgcgcagatg cgatcgctga tcaaagaggc
     4201 gatgcaggcc ggcgcagtcg gcttctcaac gacgcggtcc ggtcagcaca acggcgaagc
     4261 cggcgtgcca atgccctcgc gcctggccga tgaaaaagaa ctcatggctt tgtccactgc
     4321 gctcaaggac gcgggccacg gcgtgctcat gatgaccaag gatgcgcgtg caaccacgat
     4381 gtcctccatc gaagccatcg cgcgcgcgtc cggtcggcct tatgtggtag cggcactgct
     4441 acacagcaat gaaacaccgg aggccacgtt tgaagatctt gaggctatca agcaggcccg
     4501 cggccgcgat gcaaaactct acggggctgt cagcccttgc ccgctgaatt tcgaattcac
     4561 catgcatgaa ccttacgtgt tcgaaggtct ggccgcgtgg cggccggtca tggaagcggc
     4621 gaaggaagat gtgccacgca ttctggccag caaggcgctg cgcgacaacg tgaagaagga
     4681 actcgccgtg cgctcacggc gcatgttcaa tgggcactgg caccagatgt tcgtcgcgca
     4741 aaccgcaagg ccggaaaacc ggcacatgga atcccactcg gtgcaggaac tggcgcagca
     4801 ggcgggcaaa catccgttcg actttgcgct agacctcgcg ctggaggaaa atctcgacac
     4861 gctgttcacg gcaacgttgc tgaattccga tgacgatgcc gtgggccgca tgatgctcga
     4921 cccggccagc atgatttcgc tgtccgacgc gggcgcgcat ctgacgtttc tgtgcgatgc
     4981 cggttttggt ttacatttgc tcggccactg ggtacgcagc cgcaagctga tgccgctgca
     5041 ggaagccatc cgcaggatca cctccgaacc cgcgcagatc ttcggcatca aacagcgcgg
     5101 cacaattaag cccggaatgc atgccgatct gctgctgttc gatcccgaca cggtcgggcg
     5161 caagcgcgcg gagcgcgtgt acgatcttcc cgccggcgcg tcgcggctga cgactccggc
     5221 gaccggagtg catggcgtct gggtcaacgg aaagcaggtc gccgatgcgc atggcatacg
     5281 cgccgatgcg cccaaggccg gaaaggtgtt gcggggattt gccgcgtaac aaggtttgcc
     5341 ggctagggtg aaaaatagaa aacggacgcg ggctccacca ttcggtggaa tctttttcgt
     5401 gaagcagtcc aagagaaggc tcttctgcgc gtcagggcat cggggcgttc atggcgtttt
     5461 acgagcggcg gctgacatgg ctgttgttac atccagtagt tatcctgagc gctgccagtt
     5521 tattgggcaa gttgacagga aaaatcccgt cattaggatt gctacttctg gttaagtgac
     5581 accgaacgga gtcgagaccg ggcgccgaaa agagcattgc tcggtcgggc gcgacccgca
     5641 ataaggaggt tcgaatgacc gggacggtag tcgtattgcc ggccaaggtt cagatccagg
     5701 ggacttgcag tgacgagttc acccccgtgc gggcagcgtt cacgcataac ttggaaaccg
     5761 gccaggacgt cggcgcatcg gtcgcggtgt tcgtggaagg ccagtgcgtc gtctacctgt
     5821 ggggtggcta cctcgatggc acctacaccc ggccgttcga gcggcatacg atcgtccaag
     5881 gttactcatt gactaagacg gtcacggctc tctgcgcctt ggtcctcgcc gatcgcggcg
     5941 aaatcgattc cgacgtgcct atggcgaagt actggccgga atccgccgcg gaagggaagc
     6001 gcgatatcca gatgggtcaa ctactcggtc acagtaggcg ctgagcgtta ggtcgccaaa
     6061 acccgtggag gagacaaaca tggccaagta cagaaacgcc cttcctcaac tctgcgatga
     6121 gttcttcatg accgatgggg ggctagaaac cagtcttatt ttcct
//
LOCUS       DATMZQ010000273        13098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2640073, whole genome shotgun sequence.
ACCESSION   DATMZQ010000273 DATMZQ010000000
VERSION     DATMZQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2640073"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..720)
                     /gene="ureG"
                     /locus_tag="VN496_08915"
     CDS             complement(88..720)
                     /gene="ureG"
                     /locus_tag="VN496_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HXF17113.1"
                     /translation="MMTTQPLRVGIGGPVGSGKTALTLALCKAMRDRYQVAAVTNDIY
                     TQEDAQFLVRNEALPPERIIGVETGGCPHTAIREDASINLEAVARLARTFGNLDVVFV
                     ESGGDNLAATFSPELSDLTLYVIDVAAGDKIPRKGGPGIIKSDLLIINKIDLAPYVGA
                     SLEVMERDARNMRGDRPFLFSNLKTGQGVQDVIRFIETQGMLKAKPAVTA"
     gene            complement(739..1425)
                     /locus_tag="VN496_08920"
     CDS             complement(739..1425)
                     /locus_tag="VN496_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HXF17114.1"
                     /translation="MTATPIAASGLAQLLRLASPMLPVGAYAYSQAMEWAVEEGTVAD
                     EASAADWIAAVLRFNVGTLEAPFWVRLYHAWQENDLDAARSWNGRMLALRETAELRAE
                     TLHMGGALKAVLEGTNEIDIGLLDQLASPTFAAAFSFACHGLGVPLREGLTGYLWAWG
                     ENQVSAAMKLIPLGQSSGQRMLVKLIGMLPAIADQALNLPDERLSNFAPGLAIASSRH
                     ETQYTRLFRS"
     gene            complement(1422..1913)
                     /gene="ureE"
                     /locus_tag="VN496_08925"
     CDS             complement(1422..1913)
                     /gene="ureE"
                     /locus_tag="VN496_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HXF17115.1"
                     /translation="MLHIQKRCAPRTKFDAELVLPFDLRQKTRLRAQTTAGEEAGLFL
                     ARGSVLRDGDFLEADDGRIVKVTGKPEKILQIGCADPVQLARIAYHLGNRHVALQIGS
                     GWLRIANDYVLRQMIEGLGAVAVLTEVVFEPESGAYGGHHHDGPDAPIHRGIIHQYVS
                     AKP"
     gene            complement(1920..3626)
                     /gene="ureC"
                     /locus_tag="VN496_08930"
     CDS             complement(1920..3626)
                     /gene="ureC"
                     /locus_tag="VN496_08930"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HXF17116.1"
                     /translation="MARSITRQAYAEMFGPTTGDRVRLADTDLWIEVENDHTVYGEEV
                     KFGGGKVIRDGMGQSQRRAHEVADTVITNALIVDYLGIVKADIGIKNGRIAGIGKAGN
                     PDIQPGINIIIGAATEIIAGEGSIVTAGAIDCHIHLICPQQIEEALMSGVTTMIGGGT
                     GPATGTAATTCSPGPWHIGRMLQSLDAFPMNFGLLGKGNASLRLPLREQVAAGAMGLK
                     LHEDWGTTPAAIDNCLAVADEMDVQVAIHTDTLNESGFVETTLAAFKGRTIHTYHTEG
                     AGGGHAPDIIKACGQSNVLPSSTNPTRPYTVNTIDEHLDMLMVCHHLDAAIAEDVAFA
                     ESRIRRETIAAEDILHDLGAFSMISSDSQAMGRVGEVIMRTWQTAHKMKSQRGSLRQD
                     SAGADNFRVKRYIAKYTINPAVTHGIAHEVGSVETGKLADLVLWKPAFFGVKPSLILK
                     SGVIAAAAMGDPNASIPTPQPVHYRPMFGAFGQSCTQSSVNFVSQAGIEGDIGRKLGL
                     NKRLVPVYGTRTLQKSDLPLNGHLPHIEVDPETYEVRADGELLTCEPAKSLPMAQRYF
                     LF"
     gene            complement(3626..3931)
                     /locus_tag="VN496_08935"
     CDS             complement(3626..3931)
                     /locus_tag="VN496_08935"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HXF17117.1"
                     /translation="MIPGEIKTKDGEIEINAGRKTVTVKVANSGDRPIQVGSHYHFFE
                     TNEALKFDRGATRGFRLNIAAGTAVRFEPGQERTVELVELSGAKTVYGFQARIMGKL"
     gene            complement(3977..4279)
                     /locus_tag="VN496_08940"
     CDS             complement(3977..4279)
                     /locus_tag="VN496_08940"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HXF17118.1"
                     /translation="MDLTPREKDKLLIFTAALLAERRRSRGLKLNYPESVAFISAAIM
                     EGARDGRTVADLMSYGTTLLSRADVMDGIAELIPEIQVEATFPDGTKLVTVHHPIV"
     gene            complement(4340..5185)
                     /locus_tag="VN496_08945"
     CDS             complement(4340..5185)
                     /locus_tag="VN496_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HXF17119.1"
                     /translation="MSAALAIAPRGWEASLHLTFRHDGERSVLGRRSSAGPLAVQKAL
                     YPEGQQICHAILLHPPGGIAGGDQLHISVRIETAAHALLTTPGATKWYRSTGADSLQR
                     IGLVLAPQAVCEWMPQENIFFEAAHARNALTVDLAEDAVFCGWDVMCLGRTASGERFR
                     LGNIFQQWRVTRHGKPLFEELSILEGGSALLESPIGLAGFPVCAMFMVAGTGTDRAVL
                     EACRSTVPAGQCKWGITLMEDVLVARCLANSAQVVREYFLALWAYLRPGYAHVPARVP
                     RIWAT"
     gene            complement(5182..5880)
                     /gene="urtE"
                     /locus_tag="VN496_08950"
     CDS             complement(5182..5880)
                     /gene="urtE"
                     /locus_tag="VN496_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HXF17120.1"
                     /translation="MLKVENLNQYYGGSHTLRNIDIEVPGGAVTALLGRNGVGKTTLL
                     KCLMGVLAVSSGSITFNGQNVAKMPPYVRVRLGMGYVPQGREIFPRLTVEENLMMGLA
                     SKAHGRARDVPSEIYEMFPVLKSMRGRRGGDLSGGQQQQLAIGRALAMKPKVLILDEP
                     TEGIQPSIIKDIERVIKTLSERGEMAILLVEQYYEFARDLADHYVVLQRGEVVKSGSG
                     KNMETDGVRSFMAV"
     gene            complement(5929..6690)
                     /gene="urtD"
                     /locus_tag="VN496_08955"
     CDS             complement(5929..6690)
                     /gene="urtD"
                     /locus_tag="VN496_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HXF17121.1"
                     /translation="MRQGVDISHGPILYLDDITVSFDGFRALNKLTLTVEAGELRCVI
                     GPNGAGKTTMMDVITGKTRPDAGTAYFGQTIDLARLTEPQIAHQGIGRKFQKPTVFEN
                     HSVFENLELAIKTDKTVWASLRATLSSEQLDRIAAILDMIKLHGEADRQAGLLSHGQK
                     QWLEIGMLLMQEPRLLLLDEPVAGMTDEETERTAELFHSLAGERSLVVVEHDMEFIGS
                     IANVVTVLHEGSVLAEGTMDLIQNDERVIEVYLGR"
     gene            complement(6744..7766)
                     /gene="urtC"
                     /locus_tag="VN496_08960"
     CDS             complement(6744..7766)
                     /gene="urtC"
                     /locus_tag="VN496_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HXF17122.1"
                     /translation="MLLLLPCLYGLVPQSHAFHISAYTITLVGKIMCYAIVAMAMNLI
                     WGYTGILSLGHGVFFALGGYAFGMYLMRQIGREGQYQSDLPDFMVFLDWKSFPWYWTQ
                     SDSFLWCIALVIVVPALLALVFGFFAFRSRIKGVYFSIITQAMTFAFMLLFFRNDTGF
                     GGNNGFTDFKRVLGYPITAPSTRAVIFGVTALALIGTVVLVRYLVRSKVGRVLTAIRD
                     SESRLMFSGYNPLGYKLFVWTLSAVLCGIAGALYVPQVGIINPSEMSPANSIEIAVWV
                     AVGGRGTLIGPLLGAGVVNGAKSYFTQAFPEYWLFFLGLLFILVTLFLPKGIIGAIRF
                     INWRRT"
     gene            complement(7852..9420)
                     /gene="urtB"
                     /locus_tag="VN496_08965"
     CDS             complement(7852..9420)
                     /gene="urtB"
                     /locus_tag="VN496_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HXF17123.1"
                     /translation="MLSSVAAVAALDAQTIAKLGADDAAAKVEAVHALVASGDEDALA
                     LLQAVQAGEVAVTPQGRVFILHGDQAVDAATKMAITPLPEQFETIVINNRLRSALDAG
                     IGALKLLSPDPATRLASARAIESTSQAAVLPLIEKALARETDPTIRNSLEQTQASLNL
                     KSSDPAVRRAAVQTLGATNQARFKTVLQGLLEKDAAGNFIEPDEQVRSAAQNSIDAIQ
                     SRLVISDYIGRLFTGLSLGSILLLVALGLAITYGLLGVINMAHGEMLMIGAYATYIAQ
                     QLFKSYLPGWFDWYLVAAIPLAFAVSALVGMALERSVIRWLYGRPLETLLATWGISLF
                     LIQLVRQLFGAQNVEVANPSWMSGGIALPNLVLPYNRIVIIGFSLFVLLLMWVVLNRT
                     RLGLFVRGVTQNRAMASCVGVPTARIDLLAFGLGSGIAGLAGVALSQIGNVGPDMGQG
                     YIIDSFMVVVTGGVGQLAGTVYAALGLGVMSKFLEPHIGAVLTKILVLVLIIAFIQKR
                     PQGLFALKGRAVEA"
     gene            complement(9593..10834)
                     /gene="urtA"
                     /locus_tag="VN496_08970"
     CDS             complement(9593..10834)
                     /gene="urtA"
                     /locus_tag="VN496_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HXF17124.1"
                     /translation="MNRRHILKSLVGIAFSAMAATAIAADTIKVGVLHSLSGTMAISE
                     TALKNTALMAIEEINAKGGLLGKKLEPVVVDPASNWPLFAEKARQLITQDKVAVVFGC
                     WTSVSRKSVLPVFEELNSLLFYPVQYEGEELSQNVFYTGAAPNQQAIPAVEYLMSEEG
                     GGAKRWFLLGTDYVYPRTTNKILRAFLKSKGVGEKDIEEIYTPFGHSDYQTIVANVKK
                     FAAGGKTAVISTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRGIDTKPLLGH
                     LAAWNYFMSVKNTENTAFIKLYKDWATKNKVANLDTVVTNDPMEATYVGIHMWAQAVK
                     KAGSTDVDKVRTAMAGQTFKAPSGFTLTMDKTNHHLHKPVMIGEIKADGQFSVVWKTK
                     GPIRAQPWSPFIAGNEGKQSL"
     gene            11221..11823
                     /gene="lexA"
                     /locus_tag="VN496_08975"
     CDS             11221..11823
                     /gene="lexA"
                     /locus_tag="VN496_08975"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXF17125.1"
                     /translation="MDELTPRQAEVLRLINDFAQNTGFPPTRAEIARMLGFRSANAAE
                     DHLKALARKGFIELLAGASRGIRLKEQLGLPLIGRVAAGNPILAEANVQGRYQIDTQL
                     FKPHADYLLKVRGMSMRDAGILDGDLLAVHSTREARSGQIVVARLHDEVTVKRFKRQG
                     SQVQLLPENPDFEPIDVDLKTDLFAIEGLGVGIIRNGRLG"
     gene            12004..12444
                     /locus_tag="VN496_08980"
     CDS             12004..12444
                     /locus_tag="VN496_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17126.1"
                     /translation="METAKTVEILKALAEGIDPGTGEQFPAGSTYQQPDTVRALFSAI
                     RMLENPETSVPSRPASMKQPAATLPGKSAPQNAGRPWAEEEDTRLGNSYDAGKTIEEL
                     AEIHKRSKWAIEARLARLGKIPEPPTRFPARNAAGNATGNSVTA"
     gene            12455..12622
                     /locus_tag="VN496_08985"
     CDS             12455..12622
                     /locus_tag="VN496_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17127.1"
                     /translation="MTAVKAVSGYRSDSPRGYRGEPITGLSPITAVNTVNIVNADAWG
                     KLIRAANIKPE"
     gene            12636..>13098
                     /locus_tag="VN496_08990"
     CDS             12636..>13098
                     /locus_tag="VN496_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXF17128.1"
                     /translation="MPGPLQGVKVLDLTTVVMGPFATQLLAELGADVIKIEPHDGDNM
                     RHPGPMKNPGMGYMFLNLNRGKRGIVLDLKQAEGRAALLRLVPTADVLIYNVRPQAMA
                     RLGLSYEEVAEVNPKIIYVGAYGYGQAGPYAAKPAYDDLIQGAVGIPALSVA"
BASE COUNT         2772 a         3972 c         3617 g         2737 t
ORIGIN      
        1 cgatgcagtt gccgacaatt gctgtatcag gatcggcgag ccattacgac gcgtttcatg
       61 gcgggacagc ttaattccgg cacgagtcta tgccgtcacg gccggcttcg ccttcagcat
      121 gccctgtgtt tcgatgaatc ggataacgtc ctgcacgcct tgccctgtct tcaggttact
      181 aaaaaggaaa ggcctgtccc cgcgcatgtt gcgcgcatca cgctccatga cctccagcga
      241 tgcccccacg taaggcgcca ggtcgatttt attgataatc agcagatcgg atttgatgat
      301 gccaggccca cccttgcgtg gaatcttgtc gcctgccgca acatcgatca cgtagagcgt
      361 caggtccgac aattcaggac tgaatgtcgc ggccagatta tcgccaccgc tttcgacaaa
      421 taccacatcc agattgccaa aggtacgcgc cagccgggcc acggcctcga gattgatcga
      481 agcatcctcg cgtatcgccg tatgcggaca tccgccggtt tccacaccga tgatgcgttc
      541 cgggggcagc gcttcgttgc gcaccaggaa ttgcgcgtct tcctgcgtat agatgtcgtt
      601 ggttacggct gcgacctggt accgatcgcg catcgccttg cataacgcga gcgtcagcgc
      661 ggtcttgccc gagcccaccg gtccgccgat tccaactctc aaaggttgcg ttgtcatcat
      721 gtcacccgct aaaaatgctt atgatctgaa caggcgcgtg tactgcgtct cgtgccggct
      781 gctggcaatc gccagtcccg gtgcgaaatt gctgaggcgt tcatccggca ggttgagcgc
      841 ctgatcggca atcgcgggca gcataccaat cagtttcacc agcatgcgtt gaccgctcga
      901 ctggccgagc ggaattagct tcatggcggc tgaaacctgg ttctctcccc aagcccacaa
      961 atagccggtc agcccctcgc gcaacggcac gcccaggccg tggcaagcga aactgaaagc
     1021 ggccgcgaac gtgggcgatg caagctggtc gagcagtccg atatcaatct cattggtccc
     1081 ttcgagtacg gctttcaacg ctccgcccat gtgcagcgtc tcggcacgca actccgcggt
     1141 ctcccgcagt gccagcatgc gtccgttcca cgaacgcgcg gcatccagat cgttttcctg
     1201 ccatgcgtga tacagccgta cccaaaaggg cgcctccaag gtgccgacat tgaaacgcag
     1261 cacagcggcg atccagtctg ctgcgctggc ctcatccgca accgttcctt cttcaaccgc
     1321 ccattccatg gcctgggaat aggcgtacgc gcccaccggc aacatcggac tggccagccg
     1381 caacagctgc gcaaggccgc ttgctgcaat tggcgtggct gtcatggctt cgccgatacg
     1441 tattgatgaa tgatgccgcg gtgaatgggt gcatcgggcc cgtcatgatg atgcccaccg
     1501 taggcgcccg attccggttc aaacacgact tcggtgagca cggctaccgc ccccagtcct
     1561 tcgatcatct ggcgcagcac gtaatcattg gcgatgcgca gccaaccgga gcctatttga
     1621 agcgccacat gcctgttgcc aaggtgatag gcaatgcgcg ccagctgcac cggatcggcg
     1681 cagccgatct gcagaatttt ctccggcttc ccggtcactt tgacgatacg gccgtcatcg
     1741 gcttccagga aatcaccgtc gcgcaacacc gagcctcgcg ctaaaaacag gccagcctct
     1801 tcacccgcag tggtctgcgc gcgcaagcgc gttttctgtc ggaggtcgaa cggcagcacc
     1861 agttcggcat cgaattttgt ccgtggtgca caacgtttct ggatgtgcaa catgtgagtt
     1921 cagaatagaa agtagcgctg cgccatgggc aggcttttgg cgggctcgca ggtcagcagc
     1981 tccccatctg cgcgcacttc gtaagtctcc ggatcaacct cgatgtgcgg caagtgaccg
     2041 ttcagcggca gatcggactt ctgcagcgtg cgcgtcccat ataccgggac cagccgcttg
     2101 ttcaagccca gcttgcgccc gatgtctcct tcgatccccg cctgcgatac gaaatttacc
     2161 gaagactgcg tgcatgactg tccgaatgca ccaaacatcg gccggtaatg caccggttgc
     2221 ggcgtaggaa tggacgcatt cgggtcgccc atggcggctg ccgcgatgac gccgctcttg
     2281 agtatcaaac tcggttttac tccgaaaaag gccggtttcc aaagcacgag atcggcgagc
     2341 ttaccggttt cgacggatcc cacttcgtgc gcaatgccat gcgtgacggc aggattgatg
     2401 gtgtatttgg cgatgtagcg tttgacgcga aaattgtcgg cgcccgcgct atcctgccgc
     2461 aggcttccgc gttgcgattt cattttgtgc gccgtctgcc aggtgcgcat gatgacttca
     2521 ccaacgcgac ccatggcctg cgagtcggac gaaatcatgc tgaaggcgcc gagatcatgc
     2581 agtatatctt cggccgcaat ggtttcgcgg cgtatgcggg attcggcaaa cgccacatcc
     2641 tcggctattg ccgcatcgag gtggtggcag accatcagca tatccagatg ctcgtcgatg
     2701 gtgttaaccg tgtaaggccg cgtcggattg gtggaggacg gcaggacgtt gctctgcccg
     2761 cacgccttga tgatatcggg cgcgtgtccg ccgccggcgc cttcggtgtg ataggtatgg
     2821 atggtacgtc ccttgaacgc ggccagagtc gtttccacga aaccggactc gttcagcgta
     2881 tcggtgtgga tggcgacctg cacgtccatt tcatcggcga ctgccaggca attgtcgatt
     2941 gcggcaggcg tggttcccca gtcctcgtgc agcttcagtc ccatggcgcc ggcagcaact
     3001 tgttcccgca gcggcagacg caggctcgca ttgcccttgc cgagcaaacc gaaattcatc
     3061 gggaatgcat ccagtgattg cagcatgcgt ccgatatgcc agggcccagg gctgcaagtt
     3121 gttgccgcag ttccagtggc tggcccagtc ccgccaccta tcatggtcgt cacaccggac
     3181 atcaaagcct cttcgatctg ttgcgggcag atcagatgaa tgtggcagtc gattgccccg
     3241 gcggtgacaa tcgaaccttc tcccgcgatg atctcggttg cggcaccgat aatgatattg
     3301 atgcctggct ggatgtccgg gttgccggct ttgccaatgc cggctatgcg tccgttcttg
     3361 atgccaatat ccgctttgac aattccaagg taatccacga tcaacgcgtt ggtaattacg
     3421 gtatccgcga cctcgtgggc acgtcgttgg ctctggccca tgccatcgcg aatgactttg
     3481 ccaccgccaa acttgacctc ttcaccatag accgtgtggt cattctcaac ctcgatccac
     3541 aagtccgtgt cggccaatcg cacgcggtca cccgtagtcg ggccgaacat ctccgcatac
     3601 gcttgcctcg tgattgaacg tgccattaca gtttccccat gatgcgcgcc tgaaacccgt
     3661 acacggtttt cgcaccgctc aattccacca gttctaccgt gcgttcctgt ccgggttcga
     3721 accgcaccgc cgtgccggcc gcaatattga gccgaaagcc gcgcgtcgcg ccacgatcga
     3781 acttcagcgc ttcattggtc tcgaaaaagt gataatgaga accaacttga atcggcctgt
     3841 ccccgctgtt ggcgaccttc acggtgactg tctttcggcc cgcattgatt tcgatctcgc
     3901 catcttttgt ttttatttca cctggaatca tttgtttccc cgctgtatcc catttttgca
     3961 tccgcggtca actgcttcag acgatagggt gatgaaccgt gacgagcttg gtgccgtccg
     4021 ggaaagtcgc ctcgacctgg atttccggaa ttaattcagc aatgccgtcc atcacgtcgg
     4081 cccgcgacag cagcgtggtg ccgtaactca tcaggtcggc gaccgtccgg ccgtcacgcg
     4141 ccccctccat gatcgcggct gagatgaaag caaccgattc aggataattg agcttcaatc
     4201 cacgcgacct gcgacgctcg gccagcagcg ccgcggtgaa aataagcaat ttgtcttttt
     4261 ctcttggtgt caaatccatt gttgttgtgc ctttgcgttt tcacacagct gaccttgtag
     4321 caacgggtct tcaaccgaat catgtcgccc aaatccgcgg cacgcgcgcc gggacatgcg
     4381 catagcccgg ccgcaagtat gcccacaacg ccagaaaata ttcccgcacc acttgcgcac
     4441 tatttgccag acagcgcgcc accagcacat cttccatgag cgtgatgccc catttgcact
     4501 gccccgccgg tacggtcgag cggcatgcct ccagcaccgc gcgatcggtc ccagtgccgg
     4561 ccaccatgaa catggcgcag accggaaatc ccgccaggcc gatcggggat tcgagcaacg
     4621 cacttccgcc ttccaggatg cttagctcct cgaacagcgg ctttccgtgt cgtgtcacgc
     4681 gccattgctg gaatatgttg cctaagcgga agcgctcgcc gcttgcagtg cgaccaaggc
     4741 acatcacgtc ccagccgcag aatacagcgt cttctgccag atccacagtc aaagcattgc
     4801 gcgcgtgggc cgcttcgaaa aaaatatttt cctgcggcat ccattcgcaa accgcctggg
     4861 gggcgaggac aaggccgatg cgctgcaagg aatcggcccc cgtcgaccga taccactttg
     4921 ttgcccccgg cgttgtcagc agcgcgtgcg ccgcggtttc aatgcggacc gatatgtgca
     4981 gctgatcgcc acccgcgatg ccgcccggcg gatgcagcag gatggcgtgg caaatctgtt
     5041 ggccttcagg gtagagagcc ttttgcactg ccaacggacc tgcgctggaa cgcctgccca
     5101 gcacggaacg ttcgccgtcg tgccgaaaag tcagatgcag cgatgcttcc cagccgcgcg
     5161 gcgcaattgc cagcgcggcg ctcatactgc catgaagctg cgcacaccat ccgtttccat
     5221 gttttttccc gacccgcttt ttaccacttc cccacgttgc agcaccacat agtgatcggc
     5281 cagatcgcgc gcgaactcgt aatactgctc caccagcagg atggccattt caccacgttc
     5341 ggacaaggtt ttaatgacgc gttcgatgtc cttgatgatg gacggctgta ttccttcggt
     5401 cggctcgtcc aggatcaaaa ccttcggttt catcgccagc gcgcgaccga tcgcgagttg
     5461 ctgctgctgt ccgcccgaca aatcgccacc ccgtcgtccc ctcatggact tgagcaccgg
     5521 aaacatttca tagatctcgg acgggacgtc tcttgcgcgc ccatgcgcct tgctggccag
     5581 acccatcatc agattttctt ccaccgtcag ccgcggaaag atttccctgc cttgcggcac
     5641 gtaccccatg cccaaccgaa cccggacata aggcggcatt ttcgcaacat tctgcccatt
     5701 gaatgtaata cttccgctgg aaaccgccag cacccccata aggcatttta gcaaggtcgt
     5761 cttgccaacc ccattgcgtc cgagcagggc cgtcacggct ccgcccggta cttcgatatc
     5821 gatgttcctc agcgtatgac tgccgccgta atactgattc agattttcaa cttttagcat
     5881 gcgtcagcct tcgtaaccat gccgtgaacg aaacgcgcag cgagcacatc agcgtccaag
     5941 ataaacctcg attacgcgtt cgtcgttctg gataagatcc atcgtgccct cggcaagcac
     6001 gcttccttcg tgcaacaccg ttacgacatt cgcgatcgat ccgatgaatt ccatatcgtg
     6061 ctccaccacg accagcgagc gttcgccggc caacgagtgg aacagttcgg cagtacgctc
     6121 ggtttcctca tccgtcatgc cggcaaccgg ctcatcgagc agcagcaatc gcggctcctg
     6181 catcagcagc atgccgattt ccagccactg cttttgccca tgcgagagca gccccgcctg
     6241 gcggtccgct tcgccgtgga gcttgatcat gtccagtatt gcggcgatgc gatcgagttg
     6301 ctccgacgac agcgtcgcac gcaggcttgc ccagactgtt ttgtccgtct ttatcgcaag
     6361 ctccagattc tcgaacaccg agtgattctc gaacacggtc ggtttctgga actttcgacc
     6421 gataccctgg tgtgcgatct gcggttcggt aaggcgagcc aggtcgatgg tctggccgaa
     6481 gtacgcagtg ccggcatcag gacgcgtctt cccggtgatg acatccatca tggtggtttt
     6541 acccgcgccg ttggggccga ttacgcagcg caattctccg gcctcgacgg tcagagtaag
     6601 cttgttcagc gcgcgaaagc cgtcaaagct gactgtgatg tcatcgagat aaagaatggg
     6661 gccgtggctg atgtccacgc cttgtcgcat gacatggcta agcccggata cctcaccgct
     6721 gacgttgacc tgttcttcga tgttcatgtg cgccgccaat tgatgaatct gattgcgccg
     6781 atgatgcctt tgggtaaaaa caacgtcacc agaatgaaca gcaggccgag gaagaacaac
     6841 cagtattcgg gaaaggcttg cgtaaaataa cttttcgcgc cgttgaccac gcccgcgccc
     6901 agcagggggc cgatcagcgt gccgcgtccg cctactgcca cccataccgc gatctcgatc
     6961 gaattggcag gcgacatctc gctcgggtta ataatgccga cctgcggtac gtacagtgca
     7021 ccggctatgc cgcacaagac ggccgaaagc gtccacacaa atagtttgta tccgagcggg
     7081 ttgtacccgg aaaacatcag cctgctttct gaatcccgaa tcgcagtcag cacgcgcccg
     7141 actttggaac ggaccaggta ccgaaccagg acaacggtgc cgatcagggc cagcgcggta
     7201 acgccgaaga tgaccgcgcg tgtcgaggga gcggtgatcg ggtagccgag cacgcgcttg
     7261 aaatccgtga agccgttgtt gccgccaaaa cccgtgtcat tgcgaaagaa cagcaacatg
     7321 aaagcgaacg tcatcgcctg cgtgatgatc gaaaagtaaa cgcccttgat gcgcgaacgg
     7381 aatgcaaaga aaccgaatac cagcgccagc agtgccggca ccacgatgac cagggcaatg
     7441 caccacagga aactgtcgga ctgcgtccag taccagggaa aggatttcca gtcgaggaat
     7501 accataaaat cgggcaggtc ggactggtac tggccttcgc gcccgatctg gcgcatcagg
     7561 tacatgccaa aagcgtaacc gccaagcgcg aaaaacacac catgaccaag ggaaagaatg
     7621 ccggtatagc cccagatcag attcatcgcc atcgcaacga tggcatagca catgattttg
     7681 ccgaccagcg tgatcgtata ggctgatata tggaatgcat ggctctgcgg taccagccca
     7741 tacaagcagg gcagcaacag cagcatgacg aacgcgaaag cggccagcgc agtccaaccg
     7801 ctgcgcgtaa ataacggctc ggtgtgccgc acatatcgcg ccggcagaat ttcaggcttc
     7861 aactgcgcgc cccttcaggg caaacaatcc ctgcggtcgt ttctggatga acgcgataat
     7921 gagtaccagc acgaggattt tggtcagcac cgcgccaata tggggttcca ggaatttcga
     7981 catcacgccc aacccgagcg ccgcatatac cgttccggcc agctggccga caccgccagt
     8041 aacgacgacc atgaacgaat cgatgatgta gccttgcccc atatccggac cgacattgcc
     8101 aatctgcgaa agcgccactc cggccaatcc ggcgatgccg gagcccagac cgaaggccaa
     8161 cagatcgatg cgtgccgtgg gcacgcccac gcaggacgcc atcgcgcgat tctgcgtaac
     8221 cccgcggacg aacaagccaa gcctcgtgcg gttcagaacg acccacatca gcaacagcac
     8281 gaacagcgaa aacccgatga tgacaatacg gttgtagggg agcacaagat taggtagcgc
     8341 gatgccgccg gacatccatg agggatttgc cacttcgacg ttctgtgcgc caaacaactg
     8401 gcgcacgagc tggatcagaa acaggctgat gccccaggtt gcaagcaagg tttcaagtgg
     8461 gcgtccgtac agccagcgga tcacacttct ttccagcgcc attccaacca gcgcagagac
     8521 ggcgaatgcc aatggaatcg cagccacgag gtaccaatcg aaccagcccg gcagatacga
     8581 tttaaacaac tgttgcgcga tgtaagtcgc ataggcccca atcatgagca tttccccgtg
     8641 cgccatgttg atcacgccga gcagcccgta agtgatcgcg agtccaagcg caaccaacaa
     8701 caagatgctg ccgagtgaca ggccagtgaa caaccgcccg atgtaatcgc tgatgacgag
     8761 ccggctctgg attgcatcga tcgagttctg cgccgcggag cgcacttgct catcaggctc
     8821 gatgaaattg ccggctgcgt ctttttcgag caagccttgc aataccgtct tgaagcgcgc
     8881 ctggttggtt gcgccgagcg tttgcactgc cgcgcggcgc accgctggat ccgaactttt
     8941 gagattcagc gacgcctggg tctgttccag cgaatttctg atcgtcgggt cggtttccct
     9001 ggcgagcgct ttttcaatta gcggaagcac ggcggcctgg ctggtcgatt caatcgcacg
     9061 cgccgatgcc aatcgcgtcg ccggatccgg ggacagcagt ttcaatgcgc cgatgccggc
     9121 atcgagcgcg ctgcgcaaac gattattgat aacgatagtt tcaaactgtt cgggtaacgg
     9181 tgtgattgcc attttggtcg ctgcgtcgac cgcctggtcg ccgtgcagga tgaagacgcg
     9241 accctgcggt gtaactgcga cctcgccggc ttgcaccgcc tgcaacaagg cgagcgcatc
     9301 ttcgtcgccc gaggcaacca gcgcgtggac ggcttcgacc ttcgcggctg cgtcatccgc
     9361 ccccaacttg gcaatcgttt gtgcatcgag cgctgcaacc gccgccacgg aactcagcat
     9421 gccaatgagt gcaacaatga agaaacctgc tttcacacat gtccccggtc tgcgcacaaa
     9481 aaaaaggcct gcggatggat tgcgcaaccc gcagacctgc cctgaagtgc agcactttcg
     9541 ctgaaaagcc gggagcgcgc tcatgcgctc cgcggcctca actggatttc gattacaggc
     9601 tctgcttgcc ctcgttgccg gctatgaatg ggctccacgg ctgggcgcgg atcggaccct
     9661 tggtcttcca gacgacgctg aactgaccgt cggccttgat ttcaccgatc atgacgggct
     9721 tgtgcaaatg atgattggtc ttgtccatcg tcagcgtgaa gcctgagggt gccttgaacg
     9781 tctgccccgc cattgccgtg cgaaccttgt cgacatcggt tgacccagct tttttcaccg
     9841 cctgggccca catgtggatg ccgacataag tcgcttccat cggatcgttg gtgacgacgg
     9901 tatccaggtt cgccaccttg ttcttcgtgg cccagtcctt gtacaacttg atgaatgcgg
     9961 tgttctccgt atttttcacc gacatgaaat aattccatgc ggcaaggtgt ccgagcaaag
    10021 gtttggtatc gatgccgcga agttcttcct cgcctaccga aaatgccaca actggcacat
    10081 cggtcgcctt caggccagcg ttgccgagct ccttgtagaa cggcacgttg gagtcgccat
    10141 tgattgtgga gatcaccgcg gtcttgccac cggcggcgaa cttcttgacg ttcgcgacaa
    10201 tggtctgata gtcgctatgg ccaaacggcg tgtagatttc ttcgatatct ttctcgccaa
    10261 ccccttttga cttcaggaac gcgcgcagaa tcttgttggt ggtgcgggga tacacatagt
    10321 ccgtcccgag caggaaccaa cgcttggcgc cgccgccttc ttcactcatc agatattcaa
    10381 ccgccggaat ggcttgctgg ttcggcgcag caccggtgta gaacacattc tgcgaaagct
    10441 cttcgccttc atattgcacc ggataaaaca gcaggctgtt cagttcttca aacaccggca
    10501 gcacggattt ccgcgagact gaagtccagc atccgaatac caccgcgacc ttgtcctggg
    10561 taatcaactg gcgggctttt tccgcgaaca acggccagtt ggatgcggga tccaccacca
    10621 ccggctcaag tttcttgccg agcaatccgc ccttggcatt gatttcttcg atcgccatca
    10681 gtgcagtgtt cttcaatgca gtttcggaaa tggccatggt gcccgacaag gaatggagca
    10741 cgcccacttt gatggtatcg gctgcgatcg ccgtcgccgc catcgcagag aatgcgattc
    10801 cgaccaacga tttcaatatg tgtctgcgat tcataaagat cccctaatgt gaagtaatga
    10861 ctgcgaacgg tgagcctaaa gttgttggca cgatccggtt cgccgcttga cgcaccggtt
    10921 ctcaaatgcg cacgtcggtt tcgcaacaag cgtgccgatc gattggtgcg gcgtttaatg
    10981 tctttgcttt tcatgtcttt gtaggaaaat ttgggggcga tgacggtcca cggtgtggca
    11041 ccggttgcat caccggtgca ccggggttgt gcagccttcc ggtccgatgc acaaagttat
    11101 tgcgggacgt tatggaagca gttcagcgga atctttggcg aaaaatcgcg agatggcttg
    11161 ccagtcgtcc gatactgtat ataattacag gcatcgaagc tttatgcgga gccactcccg
    11221 atggatgaat tgacccctcg ccaggccgaa gttttgcgtt tgatcaatga tttcgcgcag
    11281 aacacaggtt ttcctccgac ccgcgccgag attgcacgca tgctcggatt tcgttctgcg
    11341 aatgccgctg aagatcatct caaggcgctg gcaagaaagg ggttcattga gttgcttgcc
    11401 ggcgcttcgc gcggcatccg cctgaaggaa cagctgggct tgccgctgat cggccgggtt
    11461 gcagcaggca accccatcct ggcggaagca aacgtgcagg gccgttatca aatcgatacc
    11521 cagttgttca agccgcatgc ggattacctt ttgaaagtgc gcggcatgag catgcgcgac
    11581 gccggcatac tcgatggtga cctgcttgca gtgcacagca cccgcgaagc gcgttccggc
    11641 cagattgtgg ttgcccgcct gcatgacgaa gtcacggtca agcgcttcaa gcgccagggc
    11701 agccaggtac agctgctgcc cgagaatccg gatttcgaac ccatcgatgt agacctgaag
    11761 accgacctgt ttgccattga aggtctcggt gtcggcatca tcaggaatgg ccggttggga
    11821 taagacagtt gggataaaaa agttgggata aacaaattgg ggtggagtga aacctctagc
    11881 tcgtagccga aagtcggatt tgcgagtcat gtccgtcacg tcgtcacgat cgtcacgtcg
    11941 tcacggattt ttaccgcaca ggtggacaaa ctagtttctt aaccacttga tcaggagaaa
    12001 ttcatggaaa cagcaaaaac cgtcgagata ctaaaagcgc tcgcagaagg aatcgatccc
    12061 ggcacaggcg aacaatttcc ggccggcagt acttaccagc aaccggatac cgtgcgcgcg
    12121 ctgttctcgg cgatccgcat gctggaaaac cccgagacct cggtgccctc ccggccagct
    12181 tcaatgaaac agcccgccgc aacgctcccc ggaaaatcag ctccgcagaa tgcgggacgt
    12241 ccatgggccg aagaagaaga cacgcgcctg ggaaattctt atgacgcagg caaaaccatc
    12301 gaagaacttg ccgaaatcca caagcgaagc aaatgggcga ttgaagcgcg tttggcacgg
    12361 ctcggaaaga ttcccgaacc gccaacgcgt tttccggctc gtaatgcggc gggaaatgcc
    12421 acgggtaact ccgtaacggc gtgattgaag gccagtgact gccgtaaaag ccgtgtccgg
    12481 ctatcgctcg gattccccga ggggataccg gggcgagccc ataaccgggc tgtcgcccat
    12541 aactgccgtg aataccgtga atatcgtgaa tgccgatgcc tggggtaaac tcatccgcgc
    12601 agcgaacatc aaaccagaat aacgaaatca cgccgatgcc aggtccattg cagggggtga
    12661 aggtcctgga cctcaccacg gttgtcatgg gtccgttcgc gacccagttg cttgccgaac
    12721 tcggcgcgga cgtgatcaaa atcgagccgc acgacggcga caacatgcgc catccgggcc
    12781 cgatgaagaa cccgggcatg ggttacatgt ttctgaattt gaaccggggc aagcgcggca
    12841 tcgtcctcga tttgaaacag gcagaaggcc gcgcggcatt gttacggctg gtgcccactg
    12901 cggacgtgct gatctacaac gtgcgcccgc aggcgatggc gcgcctcggg ctctcgtacg
    12961 aggaagtggc tgaggtcaac ccgaagatca tctatgtcgg cgcctacggc tacggccagg
    13021 ccggtccgta cgccgccaag ccggcctacg acgacctgat ccagggcgcc gtgggcattc
    13081 cggcgctgtc ggtggccg
//
LOCUS       DATMZQ010000274         9318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2186068, whole genome shotgun sequence.
ACCESSION   DATMZQ010000274 DATMZQ010000000
VERSION     DATMZQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186068"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VN496_08995"
     CDS             complement(<1..558)
                     /locus_tag="VN496_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXF17129.1"
                     /translation="MNRAVELRDNQRELYYFQLRLAIAGGAVLFAFFVLAVRFFYVQV
                     IQHEYYHTLAEQNRIFIVPVVPNRGLILDRNGVVLAHNFAAYTLEITPSLVRDLETTI
                     ADLSALVEITPKDRKRFQKLLDEGHDLASIPIRTRLNDEEVARIAVNNYRFPGVELNA
                     RLFRHYPNGEIASHVVGYIGRLSDND"
     gene            complement(599..1135)
                     /gene="mreD"
                     /locus_tag="VN496_09000"
     CDS             complement(599..1135)
                     /gene="mreD"
                     /locus_tag="VN496_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXF17130.1"
                     /translation="MMRAPMFGKLSASEEILLPVKPAYLMVTLFSALMINLLPLTGWV
                     LALRPDCVALVLLYWGIYQPRKIGFLPAWLLGLAMDVADGSLFGQHALAYSVMMFAAI
                     ALHRRVSMFDMRHQMLHVLPLLLITQLIVLGVRQAAGDAFPGWWYFVSSITGALLWPA
                     MDMLLKIPLRQRTDPDEA"
     gene            complement(1132..2034)
                     /gene="mreC"
                     /locus_tag="VN496_09005"
     CDS             complement(1132..2034)
                     /gene="mreC"
                     /locus_tag="VN496_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXF17131.1"
                     /translation="MQSSPPPFFKRGPSLFARLGFFTLLSLTLLYADDRFHYMEAARK
                     VIAVILYPLQELADSPGQAALRISNFFVSQSYLQHENDRLAHDNFLHAGQLQSQQALI
                     AENQHLRELLEIQERAEQTSMAAEILYVGRDAFSRKIIIDKGSSHGIEEGAAVVDDTG
                     LIGQVTRAFPWTAEVALVSDREQVVPVQVVRNGLRAAVFGIGYDGALDLRFMPVNTDV
                     ENGDLLVTSGIDGVYPPGLPVAVVSNIERNAAYPFAKITCTPAAGANRNRQVLVMSRL
                     APLPDYPIAPEAKKAKGKKARKGG"
     gene            complement(2146..3189)
                     /locus_tag="VN496_09010"
     CDS             complement(2146..3189)
                     /locus_tag="VN496_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXF17132.1"
                     /translation="MFGFLNGYFSNDLAIDLGTANTLIYVRGKGIVLNEPSVVAIRQE
                     GGPNGKKVIQEVGLAAKQMLGRTPGNITAIRPMKDGVIADFTVTEQMLKQFIKKVHDS
                     RLFSPSPRIVICVPCGSTQVERRAIRESAYGAGARKVELIEEPMAAAIGAGLPVEEAT
                     GSMVVDIGGGTTEVGVISLGGIVYSGSVRVGGDKFDEAIINYIRRNYGMLIGETTAEE
                     IKKEIGSAFPGSEVREKEVKGRNLAEGIPRSFTISSNEILEALTDPLNSIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDIDRLLMEETGLPVIVADDPLTCVVRGSGRAL
                     EKVDKLATIFTND"
     gene            3361..3648
                     /gene="gatC"
                     /locus_tag="VN496_09015"
     CDS             3361..3648
                     /gene="gatC"
                     /locus_tag="VN496_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXF17133.1"
                     /translation="MALTSSDVRRIAHLARIEISDAQSEQTLIQLNEIFKLIEQLQAV
                     DTSGIEPMSHPLGGSQRLRADVIGDHPDRDLVMNNAPASQDGLFLVPKVIE"
     gene            3658..5109
                     /gene="gatA"
                     /locus_tag="VN496_09020"
     CDS             3658..5109
                     /gene="gatA"
                     /locus_tag="VN496_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXF17134.1"
                     /translation="MFDKSVAELSRALLSKQISAVELARIYLDRIEQHRALNAFLDVR
                     PDVTLGQAKAADARIARGDATPLTGVPIAHKDIFVTRDFASTASSRMLQGYMSPFDAT
                     IVENLAGAGMVTLGKLNCDEFAMGSSNENSHYGNVLNPWDKNAVPGGSSGGSAAAVAA
                     RIAPAATATDTGGSIREPAAFSGVTGIKPTYGRASRWGMIAFASSLDQAGVMTCSAED
                     AALLFNGMLGFDPKDSTSVERGAEDYTRDLRSDVKGLRIGVPAEFFDAGLQPDVEKAV
                     REALAEYARLGANLVDISLPNAALGIPVYYVIAPAECSSNLSRFDGIRYGHRAERYAD
                     LDDMIKKSRAEGFGPEPQRRILIGTYVLSHGYYDAYYIQAQKVRRIIADEFQQAFRAC
                     DVIAGPVTTSVAFGFGEKAADPVAMYLSDLYTIPGSLAGIPAMSLPCGFGAHNRPVGL
                     QLMANYFEEAKLLGVAHRYQEATDWHRRMPPGI"
     gene            5120..6559
                     /gene="gatB"
                     /locus_tag="VN496_09025"
     CDS             5120..6559
                     /gene="gatB"
                     /locus_tag="VN496_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXF17135.1"
                     /translation="MNWEVVIGLETHVQLSTRSKIFSNASTQFGAPPNTQASVIDLAL
                     PGTLPVLNRGAVERAIRFGLSIGGTINTRSIFARKNYFYPDLPKGYQISQYEIPIVQG
                     GKIPIDSPTRGQIEIQLTRAHLEEDAGKSLHEDFQGMSGIDLNRAGTPLLEIVSEPVM
                     RSAAEAVAYAKALHKRVVEEIEICDGNMQEGSFRCDANVSVRRPGAPLGTRREIKNLN
                     SFRFLQQAIEYEIKWQIETLEDGGKIQQATVLFDPDKGETRMMRTKEDAHDYRYFPDP
                     DLLPVMISEEWIEQVRQSLPEKLDKQRERLLEYKIPVQQVEQLTASRSISSSALTLLK
                     GSTDKEASLLVNWLLGPISSKLKQESIEFDQLDTKLPRDSLKTLAARVAEGSISNSAA
                     GSIVLPAMWAGEGTADEIIEKKGLKQISDSGAIEKIIDEVIATHAQLVADYRNGKEKA
                     FNALVGQIMKARRNANPTQVNAILKTKLR"
     gene            complement(6564..7172)
                     /locus_tag="VN496_09030"
     CDS             complement(6564..7172)
                     /locus_tag="VN496_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXF17136.1"
                     /translation="MKSLSIIACVMTAFLAQQAHAQDKNVRMYKCVDTDGKVYYSDKL
                     NPDCAKRTEMNRQGVVVEKKELPKTGQPTKMNAPTKTSLEMERRDKALLASYTNEEEI
                     DAARDRTLVTPIQGMKTIEAKLEKANLHLSELKKQADALASQQKKLPDRLLEDVSASQ
                     KEIANLEADFAQRKAQSDSIRLKYEVEKKRFRELKGSEQPSQ"
     gene            complement(7169..7813)
                     /gene="pyrE"
                     /locus_tag="VN496_09035"
     CDS             complement(7169..7813)
                     /gene="pyrE"
                     /locus_tag="VN496_09035"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXF17137.1"
                     /translation="MSDFRQEFIDFAVARGVLCFGEFKTKAGRLSPYFFNAGLFNDGE
                     TLRLLGRFYAKAILSTDTAFDVLFGPAYKGIPLVTATSIALAGFGRNLPFCFNRKETK
                     DHGEGGLTVGAPLVGRVLIVDDVISAGTSVRESVEIIKSAGAQPAGVLIALDRMERGS
                     GKLSATQEVQDHFRIPVMSIATLDDVANFLQARAPMAQHLQSIQRYRKEYGVTT"
     gene            7823..8596
                     /locus_tag="VN496_09040"
     CDS             7823..8596
                     /locus_tag="VN496_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXF17138.1"
                     /translation="MRIVTLNLNGLRSAVNKGFLRWLAKQGADVVCVQELKAQDKDLT
                     EAMRNPGGFQGYFHCAERPGYSGVGLYCRKKPDRIISGVGIADIDAEGRYLEAQFGNL
                     SVVSLYVPSGTSSAERLEVKFSFMHRCIDPLHKLGSCGRDIVVCGDWNVAHKEIDLKN
                     WRSNRKNSGFLPEEREWFSGVIAHLGYVDVFRKLDHRPEQYTWWSNRGRAWAKNVGWR
                     IDYQMATPALAAKASAAAIYKQKRFSDHAPLSIDYDFEL"
     gene            complement(8578..>9318)
                     /locus_tag="VN496_09045"
     CDS             complement(8578..>9318)
                     /locus_tag="VN496_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HXF17139.1"
                     /translation="FMLPGIAMTLLVAEPEMAAPPKTLRAAVVEPFREFVGRAGVRSA
                     LLVLGFIFFYKLGDSMCTALATPFYLDMGFSKSDIGLIAKNAGLWPSVIGALLGGLWM
                     MKLGINRGLWLFGVVQVVSILGFAWLSRIGPFMEIGVSERTALALVIGFEALGVGLGA
                     AAFVAYIARATHPAYTATQFALFTSLAAVPRTFLNATTGWLVEQMGWFGFFLLCVLLA
                     MPGMLLLFKVAPWSANGTVLPAGQSSKS"
BASE COUNT         2048 a         2659 c         2649 g         1962 t
ORIGIN      
        1 atcgttatcg ctgaggcggc ctatgtagcc gacgacgtga gaagcgatct cgccattggg
       61 gtaatgccgg aacaacctgg cgtttagctc cacacctggg aaacggtagt tgttcaccgc
      121 gatgcgagcc acttcctcat cgttcagacg cgtgcggatc gggatgctgg caagatcgtg
      181 gccctcatct agcaatttct gaaagcgttt acggtccttt ggcgtgattt cgaccagggc
      241 ggacaaatcg gcaatcgttg tctcgagatc acgcaccagt gaaggcgtaa tctccagcgt
      301 ataagcggcg aagttgtgcg ccaggacgac cccgttgcgg tccaggatca agccgcggtt
      361 cggcacgaca ggaacaatga agatacggtt ttgctccgcg agcgtgtgat agtactcatg
      421 ctgaatgacc tgcacataga agaagcgcac agccagcaca aagaacgcga acaagactgc
      481 gccacccgca atcgcaagcc ttagttgaaa gtagtagagt tcgcgctggt tgtcgcgcag
      541 ctcgacggca cggttcattg cgagggtacg tgtcgggaag aaactgtgct gcagcggctc
      601 acgcttcgtc cggatcggtt cgctggcgca gcgggatttt cagcagcatg tccatcgcgg
      661 gccacagcag cgccccggta atgctcgaca cgaaatacca ccagccggga aacgcatcac
      721 cggccgcctg gcgaaccccg agcacgatga gttgcgtgat gagcagcagc ggcagtacat
      781 gcagcatctg gtggcgcatg tcgaacatgg atactcttcg gtgcagtgcg atggcggcaa
      841 acatcattac cgagtaggca agcgcgtgtt gcccgaacag actgccgtct gcgacgtcca
      901 tcgccaggcc cagcagccac gccggcagga atccaatttt gcgaggctgg tagatgcccc
      961 agtagagtaa taccagagca acgcaatcgg gccgcaatgc aagcacccat cctgtcagcg
     1021 gcaacaaatt gatcatcagc gcgctgaaca gcgtaaccat caggtacgcc ggcttgacgg
     1081 gcagaagaat ttcttcgctg gcgctgagct tgccgaacat cggcgcgcgc atcatccgcc
     1141 ttttctcgct tttttcccct tggctttttt ggcctcgggt gcgatcggat aatcggggag
     1201 cggcgccagc cttgacatca ccaggacctg gcggtttctg tttgcgccgg ccgctggcgt
     1261 gcaggttatc ttggcaaatg gataggcagc gtttcgctcg atgttcgaaa ccaccgcgac
     1321 cggcagcccg ggcgggtaca cgccgtcaat gccggacgtc accagcaggt ctccattttc
     1381 cacatcggta ttgaccggca tgaaccgcag atccagtgca ccgtcgtacc ctatgccgaa
     1441 tacggcagca cgcaatccgt tgcgaacgac ttgcacgggc accacttgct cgcggtcgct
     1501 cactagtgcc acttccgcgg tccacgggaa tgcgcgcgtc acctgaccga tcagcccggt
     1561 gtcgtccacc accgcggcac cttcctcgat gccgtgggag gatcccttgt cgatgatgat
     1621 ctttcggctg aaagcgtcgc ggccgacata aagaatctca gcagccatcg atgtttgctc
     1681 ggcacgttcc tggatctcca gcagctcgcg caaatgctgg ttctcggcga tcagggcctg
     1741 ctgggactgt aattgaccgg catgcaaaaa attatcgtgc gcgagtcgat cattctcgtg
     1801 ctgcaggtac gattgcgaaa cgaaaaaatt actgatgcgc agggccgctt gtccgggcga
     1861 atcggccaat tcctgcagtg gatacaagat gaccgcgatg accttgcgcg cggcttccat
     1921 gtaatggaag cggtcatcgg cgtagagcaa cgtaagagac agcagcgtga aaaaaccgag
     1981 gcgggcaaat aggctggggc ctcgtttgaa aaaaggcggt ggcgaggatt gcatctagct
     2041 ggcggtgggc actcttttcg ctatgccagg cgcgggtcgc ggtatcgagg acagatgacc
     2101 gttcgctgac tccgctcgcc taacacccgg atcccccgcg ttttgtcaat cgttggtgaa
     2161 tatggtggcc agtttgtcga ctttttccag cgcgcgtccg gagccgcgga ccacgcaggt
     2221 caaggggtcg tcggcaacga tgacaggcaa accggtttct tccattagca agcggtcgat
     2281 gtctcgcagc agcgcgccgc cgcccgtgag taccatgcct ttctcggcga tgtcggcacc
     2341 caattctggc ggcgtctgct caagggccga cttcacggcg ctgacgatgc tgttgagcgg
     2401 atcggtcagc gcttcgagaa tttcattgct ggaaatcgta aaactgcgcg ggatgccctc
     2461 ggccaggttt cgccccttga cttccttctc acggacttcc gagcccggga aagcagagcc
     2521 gatctccttt ttgatttctt cggccgttgt ttcgccgatc aacatgccgt aattgcgccg
     2581 gatgtagtta atgatggctt cgtcgaactt gtcgccgccc acccgcacgg aaccggaata
     2641 aacaatgccc cccagcgaga tcacgcctac ctcggtggtg ccaccgccga tatcgaccac
     2701 cattgaaccg gtggcctctt ccactggcag ccccgctccg attgcggctg ccatcggttc
     2761 ctcgatgagc tcaaccttgc gcgcacccgc tccataggcg gattcacgga tcgcgcgccg
     2821 ctccacctga gtggagccgc acgggacgca gataacgatg cgcggactgg gggaaaacag
     2881 gcgtgagtcg tgaacttttt tgatgaactg tttgagcatc tgctcggtca cggtgaaatc
     2941 ggctatgacg ccgtctttca tcgggcgtat cgctgtgata ttgcccgggg tccgaccaag
     3001 catctgtttt gccgccaggc ccacttcctg gatcactttc ttgccgttcg gtccgccttc
     3061 ttgccgaatt gccacgaccg aaggttcatt cagcacgata cccttgccgc gaacatagat
     3121 cagagtgttt gcagtgccga gatcgattgc gagatcgttc gagaagtaac cgtttaaaaa
     3181 tccgaacatg aggggatccg aagtttgccg cggtgcggaa aaatgaatgc cgtatgatac
     3241 cctatcgttc tgctgcagtt aataaaaacc ggggtcatcc ggttgatgtc tcgcatgaac
     3301 gacaaaatcc gggattggac cgcaaatccg cgcgtcgcgc gacttaaata gcttcgttca
     3361 atggcactca cttccagcga cgtcagacgc atcgcgcatc tggcgcgtat cgaaattagc
     3421 gatgcgcaaa gcgagcagac gctcatacag ctgaacgaaa ttttcaagct gatcgagcag
     3481 ctgcaagcag tggataccag cggcatcgag cccatgtcgc atcctctcgg gggcagtcag
     3541 cgattgcggg cagacgtgat tggcgatcac cctgaccgcg acttggtcat gaataacgct
     3601 cccgccagcc aggacggtct ttttctcgtg ccaaaagtca tcgaataggc gcgacggatg
     3661 tttgataaat cggtggccga gctcagccgc gcactgttga gcaagcaaat ttccgctgtt
     3721 gagctagcca ggatctacct cgaccgcatc gaacagcaca gagcgctgaa cgcgtttctg
     3781 gacgtgcgtc cagatgttac gctcgggcag gcgaaagcgg ccgacgcgcg tattgcccgc
     3841 ggcgatgcca ccccgttaac gggcgtgccg atcgcgcaca aggatatctt cgtcacgcga
     3901 gactttgcat ccaccgcctc gtctcgcatg ctgcaaggct acatgagtcc gttcgatgcc
     3961 acgatcgtag agaacctcgc tggcgccggc atggtgaccc ttggcaagct caactgcgac
     4021 gagtttgcga tgggttcttc gaacgagaac agccactatg gcaacgtact caacccgtgg
     4081 gacaaaaacg ccgtgccggg cggcagttcc ggtggttcgg cagcggccgt ggcagctcgg
     4141 atagcacctg cagccacggc taccgatact ggcggttcga tacgagagcc ggccgctttc
     4201 tccggggtta ccggcatcaa acccacgtat ggacgcgcgt cgcgttgggg aatgatcgca
     4261 ttcgcttcca gcctggacca ggccggagtc atgacctgct cggccgaaga cgccgcgctg
     4321 ctcttcaacg gcatgcttgg cttcgatccg aaggattcca cgagtgttga gcgcggggcg
     4381 gaagactaca cccgcgacct gcgatccgac gtgaagggtt tgcgcatcgg tgtgccagca
     4441 gaatttttcg acgccggctt gcaacccgat gtcgaaaaag cggtccgcga ggcattagcc
     4501 gaatacgccc ggctgggcgc gaacctggtg gacatttccc tgccgaacgc agcgcttgga
     4561 attcctgtct attatgttat tgcaccggcg gaatgctcct ccaatctgag ccggtttgac
     4621 ggtatacggt acgggcatcg tgccgaacgc tacgcggacc tcgatgacat gatcaagaaa
     4681 tcgcgcgccg aaggcttcgg tccggaaccc cagcgccgca tcctgatcgg cacctatgtg
     4741 ctgtcgcacg gatactatga cgcttactat atccaggcgc aaaaagtgcg tcggatcatc
     4801 gccgatgaat tccagcaagc atttcgcgcg tgcgatgtga tcgcaggtcc ggtcacgacc
     4861 agtgttgcgt ttgggtttgg tgaaaaagcc gcggatccgg ttgccatgta cctttcagac
     4921 ctctacacca taccgggcag cctggccggt atcccggcca tgagtcttcc atgcggcttc
     4981 ggcgcccaca accgtccggt aggcttgcaa ctcatggcaa actatttcga agaggcaaag
     5041 ttgcttggcg tggcgcatcg gtatcaggag gcaaccgatt ggcatcgccg catgccacca
     5101 ggcatttagg tacccgccta tgaattggga agtggtgatc ggattggaaa cgcatgtgca
     5161 gttatcgacc cgatccaaga ttttttcgaa tgcgtcgacg cagtttggcg cccctccaaa
     5221 tacacaggcc agcgtgatcg atctcgctct gcccggcacg ttaccggtat tgaaccgggg
     5281 cgctgttgag cgggcaatcc gcttcgggct gtcaattggg gggaccatta acacgcgctc
     5341 aatcttcgcg cgcaagaatt acttctatcc ggatttaccc aagggttacc agatcagcca
     5401 gtacgagata ccaatcgtgc agggtgggaa gattcccatt gattccccga cgcgcgggca
     5461 aatcgagata caacttactc gcgcgcatct ggaagaggac gccggaaagt ccttgcatga
     5521 ggattttcag ggcatgagtg gtatcgacct taaccgcgcg gggactcccc tgctggagat
     5581 cgtttcggag cccgttatgc gttcggcggc ggaagccgtt gcgtatgcga aagctctgca
     5641 taagcgggtc gtggaagaaa tcgaaatctg tgatgggaac atgcaggaag gatcgtttcg
     5701 ctgtgacgcc aatgtttcag tacgacgccc cggtgcaccc ctcggcacgc gccgtgaaat
     5761 caagaacctc aattctttcc ggtttctgca gcaggcgatc gagtacgaga tcaagtggca
     5821 gatcgaaaca ctggaagacg gcggcaagat ccaacaggcc accgtgctgt tcgatcccga
     5881 taaaggtgaa acacgcatga tgcgcaccaa ggaagatgcg cacgactatc gttattttcc
     5941 ggatccggat ttgttgccag tcatgatttc cgaggagtgg attgaacaag taaggcagtc
     6001 gttgcctgaa aagctcgaca agcagcgaga gcggctgctt gaatacaaaa tccctgttca
     6061 acaagttgag caactaacgg ccagtcgcag tatttcatca tccgcactta cgttgctaaa
     6121 gggatcaact gacaaagaag cttctctact tgtcaactgg ctcttgggcc cgatcagttc
     6181 taagttgaaa caagaaagca tagagttcga ccagctagat actaaattgc cgcgagactc
     6241 tctcaaaact ctagctgctc gggttgcgga aggaagcatt tcaaattcag cagcggggag
     6301 catcgtactt ccggccatgt gggctgggga agggaccgct gacgagatca tcgagaagaa
     6361 ggggctgaag caaatttccg actccggcgc gattgaaaaa atcatcgatg aagtgatcgc
     6421 aacacatgca caactggtag ccgattaccg caacggcaag gaaaaagctt tcaacgcgct
     6481 ggtggggcaa attatgaagg caaggcgcaa tgctaacccc acgcaggtca acgcaatcct
     6541 gaagaccaaa ttgcgctaaa gcgctattgg ctgggctgct ctgaaccttt cagttcacga
     6601 aaacgctttt tttccacctc gtacttgagg cggatggaat ccgactgggc cttgcgctgc
     6661 gcgaaatccg cttccagatt ggcgatttcc ttctggcttg cgctgacgtc ttccagcaga
     6721 cggtcgggaa gctttttttg ttgcgacgca agggcatcgg cctgtttctt gagttcgctc
     6781 aggtgcagat tcgctttttc cagttttgct tcgattgtct tcatcccctg gatgggagtg
     6841 accagagtcc ggtcgcgtgc ggcatcgatt tcttcctcgt tcgtatagga cgccagcagc
     6901 gccttatcgc gccgttccat ttccagcgat gtttttgtcg gcgcattcat tttggtcggc
     6961 tgcccggtct ttggtagttc cttcttctcg accaccacgc cctggcggtt catttccgtg
     7021 cgcttggcgc agtcgggatt caatttgtcg gagtaataaa ccttgccatc ggtatccaca
     7081 cacttgtaca tgcggacgtt tttatcctgc gcatgggcct gctgcgctag aaacgctgtc
     7141 atcacacaag caatgatgct taacgatttc atgtagtcac tccatactct ttgcgatagc
     7201 gctggatcga ttgaaggtgc tgtgccatag gtgcgcgcgc ttgcaaaaaa ttcgccacat
     7261 cgtcgagcgt ggctatgctc attactggaa tgcggaagtg gtcttgaacc tcctgcgtgg
     7321 cggacaattt accgctgccg cgttccatcc ggtccaacgc gatcaatacc ccggcgggct
     7381 gggcaccggc acttttgata atttcgaccg attcgcgcac cgatgtccct gcggaaataa
     7441 cgtcatcgac tatcaggaca cggccgacga gaggcgcgcc taccgtaagg ccgccttcac
     7501 cgtgatcttt ggtttccttg cgattgaagc aaaaaggaag gttgcgtcca aacccggcaa
     7561 gagcgatcga tgttgctgtc accaggggaa tgcctttata cgcagggcca aacaacacgt
     7621 cgaacgcggt gtctgtcgac aaaattgcct tcgcgtagaa tcgaccgagc aatcgcaagg
     7681 tctcgccgtc gttgaacaga ccggcgttga aaaagtacgg tgataaccgt ccggccttgg
     7741 ttttgaattc gccgaaacac agaacgccgc gggccaccgc aaaatcgata aactcctgcc
     7801 tgaaatcgct cattgacctc ttgtgcgcat agtcacgctc aatctaaacg gcctacgttc
     7861 ggcagtcaac aaaggcttcc tgcgctggct ggcgaagcaa ggcgcggacg ttgtctgcgt
     7921 gcaggagctc aaggctcagg acaaggatct gaccgaggcg atgcgtaatc ccgggggctt
     7981 tcaaggctat ttccattgcg ccgagcgtcc cggctacagt ggggtcgggc tgtactgcag
     8041 gaaaaagccc gatcgcataa tctctggcgt tggtatcgcg gatatcgacg ccgaaggacg
     8101 ttatctcgag gcgcaattcg gtaacctcag tgtcgtgtcg ctctatgtgc catccggcac
     8161 gagttccgcg gaacgactcg aggtcaagtt cagcttcatg catcgctgca tcgatccgtt
     8221 gcacaagctt ggcagttgcg ggcgcgacat tgtcgtctgc ggggactgga acgtcgcgca
     8281 taaggaaata gatcttaaga attggcgttc gaaccgcaag aactcaggtt ttctgcccga
     8341 ggagcgggaa tggttttccg gggttatcgc tcatcttggc tacgtggatg tgttccgcaa
     8401 gcttgaccac cgccccgagc aatatacctg gtggtcaaac cgcggtcggg cttgggccaa
     8461 gaacgtgggc tggcgcatcg attatcagat ggcgacgcca gcgctggcgg ccaaagcgag
     8521 cgcggccgcc atctacaaac agaagcgttt ctcggaccac gccccgctca gtatcgatta
     8581 cgacttcgag ctctgacctg ccggcagaac agtgccgttt gcgctccatg gcgcgacttt
     8641 gaacaggagc agcatgccgg gcatcgcaag taatacgcaa agcaaaaaaa agccgaacca
     8701 acccatttgc tccaccagcc atccggttgt tgcgttcaga aatgtgcggg gcacggcggc
     8761 gagactggtg aaaagcgcga actgggtcgc cgtataggca gggtgggtcg cgcgtgcgat
     8821 atacgcgaca aacgcagcag cgcccaagcc aacccccagg gcttcaaaac cgatcacgag
     8881 cgccaacgcc gttcgctctg ataccccgat ttccataaac ggtccgatgc gagacaacca
     8941 ggcaaagccg agaatcgaca caacctgaac cacaccgaag agccacaatc cacggttgat
     9001 tcccagtttc atcatccata gcccgcccag caaggctccg atcacgctgg gccatagtcc
     9061 agcgtttttt gcgatcaacc cgatgtccga cttggaaaac cccatgtcga gataaaaagg
     9121 tgttgcaagc gccgtgcaca tgctgtcgcc cagcttgtag aaaaaaatga aaccgaggac
     9181 cagcagcgca ctgcgcacac cggcccggcc cacgaactcg cgaaacggct cgaccaccgc
     9241 cgcacgcagc gttttcggcg gggccgccat ttccggttcg gcgaccagca aggtcatcgc
     9301 tatgcccgga agcatgaa
//
LOCUS       DATMZQ010000275         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4948201, whole genome shotgun sequence.
ACCESSION   DATMZQ010000275 DATMZQ010000000
VERSION     DATMZQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4948201"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VN496_09050"
     CDS             complement(<1..953)
                     /locus_tag="VN496_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF17140.1"
                     /translation="MKRTRILNGALSILLLGTITLAQAPSSEVDTHIATAKAAAGQDY
                     RGTFVNLCLPGGAPGGGARGAGGAPGGGARGAGGAPGGGARGATGAAPAARGATAAAT
                     PDRAGWYASPYKVFDNLYWLGTRQHSSWALRTSAGLILIDTNFAWATQPEIIDGLAKL
                     GLNANDLKYVIISHAHGDHDQGAAELQAKYGAKVVMGAADWDSTLQRPATAAGGVPKR
                     DITVGPEGTKITLGDTTVTIVHTPGHTPGTLSYVFPVKDAGRTVMVAYSGGTLTGAFG
                     TDGSRWDEYIASQKKIAKAAADAGASVILSNHSEYDNAYTKA"
     gene            complement(1188..>2789)
                     /locus_tag="VN496_09055"
     CDS             complement(1188..>2789)
                     /locus_tag="VN496_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HXF17141.1"
                     /translation="YDPFYSRLLSTLVSALPVLTLFFVLVVLKKRVWISALSGLMVAV
                     ALALLVFKMPAVLVVTAAGHGFIFGLLRIAWIVVGSIFLYNVASETGQFQVMKDSIAG
                     LSSDKRLQLVLIAFCFGAFLEGTGGGGAPVAIAGAFLMGLGFSPFQAATLCLLANTAP
                     VAWGGVGNPIRTLGGVTGLPELQFSAMAGRILPPLSLILPFWLVRSMVGWRETFQVWP
                     ALLVSGLSFAGMQFYWSNFQDIGLVDVVSAVFSLLLTTAFLKFWKPAKVIPVSGELAV
                     SHRRHSAAQVLKGWSPFIVASILIFVTGLPSMSKYLNVNALKMPMPYLHNAVLKMPPV
                     APVPTPEDATVNLNFLGIPGTVVFVAGIVAALLAGLPLGKTARIFKATVVQMMPSLLA
                     ISFMVGLAYVTRYCGMDTIIGLSLTGTGKLYPFFGTLLGWLGVALTGTDAGSNALFGN
                     LQKVTAEQLGLSPILMGAANSTGGVMGKMIDAQSIVVSSSATKQQGNEAAIFKAVFWH
                     SIALASLVGVIVMFYAYVIPWAIPH"
BASE COUNT          639 a          828 c          767 g          555 t
ORIGIN      
        1 gctttcgtgt aggcgttgtc atattcgctg tgattcgaca ggatcactga cgcgccggca
       61 tcggccgccg ctttggcgat tttcttttgt gaggcgatgt attcatccca ccgcgagccg
      121 tctgtgccaa aagcgccggt cagagtaccg ccggaatacg cgaccatcac tgtcctgcct
      181 gcatccttca ctggaaaaac ataagacaat gttcccggtg tgtgacctgg cgtatgcacg
      241 atcgtgactg ttgtgtcgcc aagcgtgatt tttgttcctt ccggtccgac agtgatgtcg
      301 cgcttaggaa cgccacctgc ggcagtagcg ggccgttgca acgtggaatc ccaatccgcc
      361 gcgcccatga cgaccttggc gccgtatttt gcctgcagtt ccgccgcacc ttgatcgtga
      421 tcgccatgcg catggctgat gatgacgtac ttgaggtcgt tcgcgttcaa gccgagcttc
      481 gcgagcccat cgatgatttc cggttgggtg gcccatgcga agtttgtatc aatgagaata
      541 aggcccgcgc ttgtgcgcag cgcccaggac gagtgttggc gcgtgccaag ccagtagaga
      601 ttgtcgaaca ctttataggg tgaggcatac cagcccgcgc gatccggagt ggctgccgca
      661 gtggcacctc gcgcggccgg tgctgcgccc gttgcgccgc gagcgccgcc tcctggcgca
      721 ccgcccgcgc cgcgagctcc accgccgggc gcgccacccg cgccgcgggc tccaccgccg
      781 ggcgcgccac cgggcaggca gagattcacg aacgtgccac ggtaatcctg ccccgcagcg
      841 gcctttgccg ttgcgatatg cgtatccact tcgctggaag gcgcctgagc gagggtaatg
      901 gttccaagaa gcagaatcga taacgcgccg ttcaaaatac gtgttcgctt cattttattc
      961 accgaaaact taagagacat ttgagccgct ccaggttcat ccgcagtttc caccaatgac
     1021 ttccggagcg tgaacgaggt tgccttggaa ggagcacttt ccttcctagt cgatattagg
     1081 aaaattcaag cgtgccaata ttagttccgt cgcccagcag attcaatcac cggactaata
     1141 ctttccacaa agtcgttgga agtcttatcc cggtattgcc gagactctca gtgtggtatc
     1201 gcccagggga taacatacgc atagaacatc acaatcactc ccaccagcga tgccaacgca
     1261 atgctgtgcc agaatactgc cttaaagatc gccgcttcgt tgccttgctg tttggtcgcg
     1321 gaactcgata cgacgatcga ctgggcatcg atcatcttgc ccattacgcc gccagtggaa
     1381 tttgcagctc ccatgaggat ggggctcaag cccagctgct ccgcagtgac tttttgaagg
     1441 ttcccgaata gggcgttcga gcccgcgtct gtgcccgtca gggccacgcc gagccagccg
     1501 agcaacgtgc cgaagaatgg gtagagcttt cccgttccgg tcaaactcaa gcctatgatc
     1561 gtatccatgc cgcagtatcg cgttacgtac gcgaggccga ccatgaagct gatagccagc
     1621 aacgacggca tcatctgcac gactgtcgct ttgaaaatac gcgcggtttt acccagaggc
     1681 aaacccgcaa gcagggccgc aacaatccca gcaacgaaga caactgttcc aggtataccc
     1741 aggaagttta aattcaccgt ggcatcttca ggtgtgggta ctggagcgac cggaggcatt
     1801 tttagaacag cgttatgcag gtagggcatt ggcattttca gtgcattgac gttcaaatat
     1861 ttcgacatcg acggcaggcc cgtcacgaaa atcaaaatcg aggcgacgat gaagggcgac
     1921 catcctttta gcacttgcgc cgctgagtgg cgtcggtggg aaaccgccaa ttcaccgctg
     1981 acggggatca ctttggcggg cttccagaac ttgagaaacg ccgtcgttaa cagcagcgaa
     2041 aataccgccg aaaccacgtc aacgaggcca atgtcctgaa agttggacca gtagaattgc
     2101 attccggcaa aggagagccc gctgaccagc aacgcaggcc acacctggaa tgtttcgcgc
     2161 catccgacca tgctgcgtac cagccagaag ggcaggatca gagacagagg cggcagtatc
     2221 cggccagcca tcgcgctgaa ctgcaactcg ggcaatccgg tcacgccgcc gagggtgcgg
     2281 attggattac ccacgccgcc ccatgcaaca ggtgccgtgt ttgccagcag gcaaagcgtc
     2341 gccgcttgaa atgggctgaa gcccaatccc atgagaaacg ctccagcgat tgccacgggc
     2401 gcgccgcctc ctccggtacc ttcgaggaag gcaccaaagc aaaacgcaat cagcacgagc
     2461 tggagacgct tgtccgagga cagtcccgcg atcgaatcct tcataacctg gaactgtcct
     2521 gtttcggacg cgacgttgta gagaaaaatc gagccgacta cgatccatgc aatgcgaagc
     2581 agcccgaaaa taaacccatg tccggccgcg gttactacca gcaccgcagg cattttgaaa
     2641 accaatagcg cgagcgcgac tgccaccata agtccgctca aagcggaaat ccacacgcgc
     2701 tttttcagta ccaccaggac aaagaaaagc gtcaacaccg gcaatgcaga gaccagcgtc
     2761 gaaagcagtc tgctatagaa cggatcgta
//
LOCUS       DATMZQ010000276         6162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_573805, whole genome shotgun sequence.
ACCESSION   DATMZQ010000276 DATMZQ010000000
VERSION     DATMZQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573805"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VN496_09060"
     CDS             <1..2047
                     /locus_tag="VN496_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF17142.1"
                     /translation="AVAIPVSVIGHFTFLKAMGFSINTLTLLGVVLSIGLVVDDAIVV
                     LENIHRHIEEGMQPLKAAFLGSKEIGFAVIAMTITLAAVFTPLALMTGNTGKLFTEFA
                     LTVATSVIVSGFVALTLTPMMCSKLLKSGHSSVYTYTERFFEGMNNGYRRILRGTLRH
                     RWVIGVIFVVVLGSMVLLFKQLKAELSPVEDRGLFLAFVVAPEGSTMSYTDGYMHVVE
                     GITAKIPEITTMFAVVAPGLDRPNPVNLGVAFAVLKPWSERERSQLTITKELGPKLYG
                     GLPGALSFALNPPSLGQGLLTKQIEYVIYGNSFEELQGQVNLVMAKLREYPGITALDT
                     DLKLNKPQMVVEINRDKASDLGVSMDVIGHTLQTLLGSRDVTRYKHEGKQYDVVVQLE
                     DSKRMQPTDLTSIYVRGRDDQLMQLSNLVKLSEAVAPKELNHFNKLRAAVINGNVASG
                     YTLGQALEVIDATVKQELPAGIITDLDGQAREFREAGGQLYLTFVLALVFIYLVLSAQ
                     FESFVGPLVIMFTVPLAMTGALLAMWINAMLGHGGTLNVYSQIGLVMLVGLITKHGIL
                     IVEFSNQLRSRGMEKFEAVVEAATLRLRPILMTTGAMVLGAIPLALAHGAGAESRQSI
                     GWVIVGGLMVGTLLTLFVIPTIYTLLMRKVHMADEEEVIVPLTAGGSGPITAPHKEL"
     gene            2158..3060
                     /locus_tag="VN496_09065"
     CDS             2158..3060
                     /locus_tag="VN496_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXF17143.1"
                     /translation="MRLRFYTLDVFTDRPFGGNPLAVVLDADLLSGERMQSIAAEFNL
                     SETVFVLKPAKAGANRKKLRIFTPQAELPFAGHPTIGTSYLLAKLKLVELHALDSIMV
                     LEEAIGDVPVKVEYGADGPRSTRMSVPKMPERGPEPPRLADLAAMLSLAEADLLPGHA
                     AYSCGVPFLFLPVRDLAAIARVKLRGDLWERLLSGWWAKSVFVFTAETVAQDADIHAR
                     MFAPAIGITEDPATGGAAAALAGYLHAQDRHEGRRRWLVEQGFEMGRPSNIELEADAS
                     GGIITAVRVGGSSVLMSEGWCEAG"
     gene            3186..3884
                     /locus_tag="VN496_09070"
     CDS             3186..3884
                     /locus_tag="VN496_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXF17144.1"
                     /translation="MNALRKSSERGFADHGWLKSFHTFSFAGYHDPRQMGYGALRVIN
                     EDRVAPGQGFGKHPHRDMEIISYVLEGELEHQDSMGTGSVIRPGDVQRMSAGTGVFHS
                     EYNPSSMNGVHFLQIWIEPDTLGVKPSYEQKFFAPEEKRGKLRLVASPDARDGSVSIH
                     QDAYLYAALIDGAEQVVHRLESGRRAYVHVARGSVTVNGQPLGAGDALKVDGESTVAM
                     SDGDNAEVLLFDLE"
     gene            complement(3950..5359)
                     /gene="boxB"
                     /locus_tag="VN496_09075"
     CDS             complement(3950..5359)
                     /gene="boxB"
                     /locus_tag="VN496_09075"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HXF17145.1"
                     /translation="MSINYSERIPNNVDLANDRTLQRALEHWQPKFLQWWREMGPHET
                     ESAEVYLRTAVGVDAQGWASYGAVKMPDYRWGIFLAERMPDRTIGFGDHYGEPVWQQV
                     PGELRSQFRRIIVTQGDTEPASVEQQHRLGLTCPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLHAHFGRDGREEAEELLARHSGDVDKPRILGTFNEPISDWLSFFMFTYFTDRDGKFQ
                     LKSLAESGFDPLARTCRFMLTEEAHHMFVGETGVGRIVKRTLEVMKEIGSDDAQGVRD
                     HGAVDLATIQRYMNFWFTSSLDLFGSEVSSNAASSFASGIKGRPDETQYEDHVCSDAV
                     LSLETPQGQESVSLRNAMNEVMRQSYIRDCEIGLKRWNMQIQRAGYDFRLALPSPHFR
                     RAIGAWVNVPTDPQGKPISREAYERRMPEWLPSEADRTFVHSLMQRVVEPGKMAGWIA
                     PPDRGINNLGLDYQYVKLS"
     gene            complement(5449..>6162)
                     /locus_tag="VN496_09080"
     CDS             complement(5449..>6162)
                     /locus_tag="VN496_09080"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745258.1"
                     /note="cleaves the ring of
                     2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming
                     6-hydroxy-3-hexenoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HXF17146.1"
                     /translation="ILMLRTNDLAIGVWLLKTRGDTTSVLACDAMLAKHCNDWFVRET
                     TGFLRRTLARLDVSSRTLFAVIDQGSCFAGALAELVFAADRSYMLQQENDAATLVLTE
                     LNFGIVPMVNGLSRIANRFCDETDQVDAVRAHVGEPLDAAAASAMGLVTYAPDDIDWD
                     EEIRIAIEERTSLSPDALTGMEANLRFTGAETMATRIFGRLTAWQNWIFVRPNAVGEH
                     GALKVFGSGSKAKFDWERV"
BASE COUNT         1233 a         1762 c         1840 g         1327 t
ORIGIN      
        1 cgctgtcgcc attccggtgt cggtgatcgg ccacttcact ttcctcaagg ccatgggctt
       61 ttcaatcaat acgctgacat tgctcggcgt ggtgctgtcg atcggcctgg tggtggacga
      121 tgctatcgtc gtgctggaaa acatccaccg ccatatcgaa gagggtatgc agccgctcaa
      181 ggcggccttc ctcggcagca aggaaatcgg ttttgcggtg attgccatga ccatcacgct
      241 ggccgccgtg ttcacgccgc ttgccttgat gaccggcaat accgggaagc tgtttacgga
      301 gtttgcgctt accgtggcca cgtccgtaat cgtttccggg tttgtcgcgc tgacgttgac
      361 gccgatgatg tgctcgaagc tgttgaagtc aggccatagc agcgtctata cctacaccga
      421 acggttcttt gaaggcatga acaacggcta tcgccgtatt ctgcgtggca cgctgcgtca
      481 tcgctgggta atcggggtta tcttcgtcgt ggttcttggc agcatggtct tgctgttcaa
      541 acagctgaaa gccgaacttt cgccggtgga agaccgtgga ttatttctgg cttttgtggt
      601 ggcgccagaa ggttccacca tgtcctacac cgacggctac atgcatgtgg tcgaaggcat
      661 caccgccaaa attcccgaaa ttaccaccat gttcgccgtg gtcgcaccgg gcctcgaccg
      721 cccaaacccg gtcaatctcg gcgttgcttt tgctgtcctg aaaccctgga gcgagcgcga
      781 gcgcagccag ttgaccatca ccaaggaact tggcccgaaa ctttacgggg gcctgcctgg
      841 cgcgctgtcc tttgcactca acccaccttc gctgggacaa ggcctgctga ccaagcagat
      901 tgaatacgtg atttatggaa acagttttga agaactgcag ggccaggtaa atcttgtcat
      961 ggccaaattg cgcgagtatc cgggcattac ggcgctcgac acggatttga aactcaacaa
     1021 gccgcaaatg gtggttgaaa tcaatcgcga caaggcttcc gatctgggcg tatccatgga
     1081 cgtcatcggg cataccctgc agacgctgct tggcagccgc gatgtgacgc gctacaagca
     1141 cgagggcaag cagtacgatg tggtcgtgca gttggaagac agcaagcgca tgcagcctac
     1201 cgatctgact tccatttatg ttcgcggccg ggacgaccag ctcatgcagc tttccaacct
     1261 ggtgaagctc agcgaggcgg ttgcgccgaa ggaactgaac cacttcaaca aactccgggc
     1321 cgccgtaatc aacggcaacg tcgcctccgg gtacacgctt ggccaggcgc tagaggtgat
     1381 tgacgcgact gtcaaacaag aactgcccgc gggcatcatt accgatctgg atggacaggc
     1441 gcgcgagttt cgggaagcgg gcggccagtt gtacctgacg tttgtactgg cgctggtatt
     1501 tatttatctg gtgttgtccg cgcaatttga aagtttcgtc ggaccgctgg tcatcatgtt
     1561 taccgtaccg ctcgcaatga ccggggcgct gctggcgatg tggatcaatg ccatgctggg
     1621 ccacggcggc acattgaacg tgtatagcca gatcggtctg gtgatgctgg tgggcctgat
     1681 taccaaacac ggcattctga tcgtcgaatt ctccaatcag ctgcgcagcc gcggcatgga
     1741 aaaattcgaa gcggtggtgg aagcagcgac cttgcgcctg cgtcccattt tgatgacgac
     1801 cggcgcgatg gtgctcggag cgattccgct ggcgctggct catggtgccg gcgccgaatc
     1861 aaggcaatcc atcggctggg ttattgtcgg cgggttgatg gtgggaacgt tactgacttt
     1921 gttcgtgatt ccaaccatct acacgctcct gatgcgcaaa gtacacatgg ccgacgagga
     1981 agaagtcatt gtgccgctta ccgccggcgg cagcggacca ataactgcgc ctcacaagga
     2041 actgtaacca ggcgcggcgc cagtcagcgt cagaaacggc gaaggcaaat gcgttcgccg
     2101 tttttttttg gctatagtgc agctgaacca taacggaagt ttcttcaatt caccgcaatg
     2161 cggttgcgct tctacaccct ggatgtgttt accgatcggc cgttcggcgg caatccgctt
     2221 gccgttgtcc tggacgcgga tctgttgagt ggcgagcgca tgcaaagcat cgcagccgaa
     2281 ttcaatttgt cggaaacggt attcgtatta aaacctgcga aggcgggcgc caatcgcaag
     2341 aaactgcgca tcttcacacc ccaggctgaa ctgccgtttg caggacatcc gacgatcggt
     2401 accagttatt tactggcgaa actcaagctg gttgagttgc atgccctgga ttccatcatg
     2461 gtgctggaag aagctattgg cgacgtcccg gtcaaagtgg agtacggcgc ggacggaccg
     2521 cggtcgacgc gcatgtcggt ccccaaaatg ccggagcgcg gaccggaacc gccaagactt
     2581 gcggacctgg cggcaatgct ttccctggct gaagccgatc tgctgccggg acacgccgcc
     2641 tattcttgcg gcgtgccatt tttgtttttg ccggttcgcg atcttgccgc catcgcgcgc
     2701 gtcaagctgc gcggcgactt gtgggaaaga ctgctgtccg gttggtgggc caagagcgtt
     2761 tttgttttta ccgccgaaac ggtcgcgcaa gacgcggaca ttcacgcccg catgttcgcc
     2821 ccggcgatag gcatcacgga ggaccctgca actgggggtg cggccgccgc gctggccggg
     2881 tacctgcatg cgcaggatcg ccacgaaggc aggcgcagat ggctggtgga gcagggcttc
     2941 gaaatgggcc gtccgtccaa catcgaactc gaagcggacg cttccggcgg gataatcacg
     3001 gccgtgcggg ttggcggcag ctccgtgctg atgtccgagg gctggtgcga agccggctga
     3061 tgtcgttcta aaaattcgac ttatcggttc aaatactccc gctaatcgtt tcggccggat
     3121 gccgctatat tgatcgaatc gacaaaggct ttgccgaggt ggccaggcct gaacaggaga
     3181 catcaatgaa tgctttgaga aaaagcagcg agcgcggatt cgccgaccat ggctggttga
     3241 agagtttcca taccttctca ttcgccggct accacgatcc caggcaaatg ggttatggcg
     3301 cgctgcgcgt gatcaacgaa gaccgggtcg cgccaggcca ggggtttggc aagcatccgc
     3361 atcgggacat ggagatcatc agctatgtgc tggagggcga gctggagcac caggacagca
     3421 tgggcaccgg ctcggtcatt cgtccggggg atgtgcagcg catgagcgcc ggaactggcg
     3481 ttttccatag cgagtacaac ccctcttcca tgaacggcgt gcacttcctg cagatctgga
     3541 ttgagcccga tacccttggt gtcaagccca gctacgagca gaaatttttt gcacccgagg
     3601 aaaagcgcgg caagctgcgc ctggtggctt caccggatgc gcgcgatggt tcggtatcga
     3661 ttcatcagga tgcttacttg tacgccgcac tcattgatgg agccgagcag gtcgtgcatc
     3721 ggcttgagag cggacgccgc gcttacgtgc acgtggcgcg cggcagcgtt accgtgaatg
     3781 gccagccgct gggcgccggc gacgcgctga aggtcgatgg tgaatcgacg gtcgccatga
     3841 gcgacggcga caatgcagag gtactgttgt tcgatctgga ataatccaac cagtgcgaca
     3901 acgacagcgg caggctatcg tgcctgccgc tgcgcttttc gagttccaat taactcagct
     3961 tgacgtactg gtaatccagt ccaaggttgt taatgccgcg atcgggcggc gcaatccagc
     4021 cggccatttt ccccggctca acgacgcgct gcatgaggct gtgcacaaac gtgcggtccg
     4081 cctcgcttgg cagccattcc ggcatgcgtc gctcatatgc ctcacggctg atgggcttgc
     4141 cttgcggatc ggtaggcacg ttgacccagg cgccgatggc gcggcggaaa tgcgggctgg
     4201 gtaatgcgag ccggaaatcg tacccggcgc gctgtatctg catattccag cgcttgaggc
     4261 cgatttcgca atccctgatg tatgactggc gcatgacttc attcatcgca ttgcgcaggc
     4321 tgaccgactc ctggccttgc ggcgtttcca gcgagagtac tgcatcgctg cagacatggt
     4381 cctcgtactg cgtttcgtcg ggccggcctt tgatgccgct ggcaaacgaa cttgccgcat
     4441 tcgacgaaac ttcggagccg aacaggtcga gcgatgaggt aaaccaaaaa ttcatatagc
     4501 gctgaatggt agcgaggtcg actgcgccat ggtcgcgcac accctgcgcg tcgtcgctgc
     4561 cgatttcctt catgacttcc agcgtacgtt tcacgattcg gccgacgccg gtttcgccga
     4621 cgaacatgtg atgcgcttcc tccgtgagca tgaagcggca ggttcgtgca agtggatcga
     4681 agccggattc ggccaggctc ttcagctgga attttccgtc gcggtcggtg aaatacgtga
     4741 acatgaaaaa cgacagccag tcgctgatcg gttcgttgaa ggtaccgaga atgcgcggct
     4801 tgtctacatc gccggaatgc cgcgccagca gttcctcggc ttcctcgcgc ccgtcgcggc
     4861 cgaaatgggc gtgcagcaga tagaccattg cccacaggtg cctgccttcc tcgacgttga
     4921 cttggaacag attgcgcagg tcgtacagcg aagggcacgt caggccgaga cggtgctgct
     4981 gttcgaccga tgcgggctcc gtgtcgccct gcgtgacgat gatgcggcga aactgcgaac
     5041 gcaattcgcc cgggacttgc tgccagacag gttcaccgta gtgatcgccg aagccgatgg
     5101 tgcggtctgg catgcgctcc gccaggaata tgccccaacg gtagtccggc atcttcaccg
     5161 cgccgtaact ggcccagccc tgtgcatcga cgcccaccgc cgtacgcaga tacacttccg
     5221 ccgattcggt ttcgtgcggt cccatttcgc gccaccattg caggaacttc ggttgccagt
     5281 gttcgagggc gcgctgcaac gtgcggtcat tcgccagatc gacgttgttg ggaattcttt
     5341 cgctgtaatt gatgctcatg gtcggctcga attcatgaat gtgagtgcga acgggtctat
     5401 tgcttgttac caaagcgaac gctgttgcgt atgacggccg ggttccatct aaaccctttc
     5461 ccagtcgaac ttagctttgc tgccgcttcc gaatactttt aacgcgccgt gttcgccgac
     5521 ggcattgggc cggacaaaaa tccagttttg ccaggcagtc agacggccga aaatgcgcgt
     5581 cgccatggtt tccgcaccgg tgaaacgcaa gtttgcttcc atgccagtca gcgcgtcagg
     5641 tgaaaggctg gtccgctctt cgatggcgat gcgtatttcc tcgtcccaat cgatgtcgtc
     5701 cggcgcgtaa gtcaccagac ccatcgccga ggcagcggcc gcatcgagtg gctcgccaac
     5761 gtgcgcgcgc accgcgtcaa cctggtccgt ttcgtcgcag aaacggttgg cgatgcggct
     5821 cagtccattg accatcggga caataccgaa attcagttcc gtcaggacca gggttgccgc
     5881 atcattttcc tgttgcagca tgtagctgcg atccgccgcg aaaaccagtt ccgcaagcgc
     5941 gcccgcgaag caggaaccct ggtcgataac ggcaaacagc gtgcgcgaag acacatccag
     6001 ccgcgccagc gtccgtcgta gaaaaccggt ggtttcgcgc acgaaccagt cattgcaatg
     6061 cttcgccagc attgcgtcac aggccagtac cgacgtcgta tcaccccgcg tcttgagtaa
     6121 ccagacaccg attgcaagat cattggtacg cagcatcagg at
//
LOCUS       DATMZQ010000277         4958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2642558, whole genome shotgun sequence.
ACCESSION   DATMZQ010000277 DATMZQ010000000
VERSION     DATMZQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642558"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VN496_09085"
     CDS             complement(<1..625)
                     /locus_tag="VN496_09085"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXF17147.1"
                     /translation="MKLVSWNIQWARGIDLAVDPARIVRTANKLADFDVLCLQEVAAN
                     FPGLPGSRGEDQVRELSLGLPGYSAHYGVATDIDDGQGGRSLFGNVLFSRLPVLQVYR
                     HLLPWPADPAADSSMQRIALEAVVAAPWGPIRITTTHLEYYSATQRMAQVDALRALHQ
                     QACQRGAEAQKAKSAQGPFQMRPTPVSAVLTGDFNCSPDALEYRRTIE"
     gene            complement(637..1488)
                     /locus_tag="VN496_09090"
     CDS             complement(637..1488)
                     /locus_tag="VN496_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF17148.1"
                     /translation="MPELNVPAGRALPGGLSTCIALAVGLGFCISVALAADTVYPGWH
                     DTPDPQGPVGDAIALGRRVLTNTAQSAPAYSGNALNCTSCHLNAGRTPWSSPWVGIWG
                     VFPEYRSRSARVISLQDRINDCFERSMNGKALPPASAEMIGILAYMQWLSRDVPTGQS
                     VTGRGFLRMKSTQPPDRERGARIYAHKCAVCHGEQGQGMPAPSGDMLYPPLWGPKSFN
                     IGAGMARLNTAAAFVKANMPFDQPGSLSDEEAFDVAAFFTRQPRPDFAGKSADWAQGG
                     KPEDARY"
     gene            complement(1599..1742)
                     /locus_tag="VN496_09095"
     CDS             complement(1599..1742)
                     /locus_tag="VN496_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17149.1"
                     /translation="MCFEFEALYWAKLAEEEDKRRQAEKQRNAPTPEPQKEPAYEQDP
                     LPV"
     gene            complement(1848..2510)
                     /locus_tag="VN496_09100"
     CDS             complement(1848..2510)
                     /locus_tag="VN496_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HXF17150.1"
                     /translation="MSFQIDIVSDVVCPWCFIGKRNLERALDQWRARYPDEVPIVRWH
                     PFQLNPELPADGVPRKQYLENKFGGPERAREIYARVSTAGKRAGIEFAFDAIQVQPNT
                     VDAHRLMHYAGERGKQDAMAEALFRRYFEEGADLTDKAELADIAAQAGINRDDASTYL
                     ASDEDRALIEEQDRRARATGVQGVPFFIFNQRLALSGAQPPEIMVEALEKVREHLAAM
                     PQ"
     gene            complement(2535..2966)
                     /locus_tag="VN496_09105"
     CDS             complement(2535..2966)
                     /locus_tag="VN496_09105"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HXF17151.1"
                     /translation="MTGVHMTRYLMAVALASMCCATRAEVLCPDSLEVNQQANIAGDW
                     TVSYTDPPLRLTGVQIYDGPPSQNHRIKPYSIKSVKGELRISWRFPESKRNFHLACTY
                     ERTTASLTALLPPGMNGCNAVFDRRVSYGNAALAVKRMVCN"
     gene            complement(2981..4375)
                     /locus_tag="VN496_09110"
     CDS             complement(2981..4375)
                     /locus_tag="VN496_09110"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXF17152.1"
                     /translation="MAIAKTGLFLRQLILVCAALSATAALALVTQREPATSGESFTTD
                     DASPQAYSLPGSGLDTAQLELFAGGGQEFEQRWVVLLGIGGKWGRGPTSNAERCADCH
                     INNGRGHSPDVEGETLASMLVRLSIPGQDPHGAPLPHPNYGEQLQNQGELGRVPAEGN
                     AVIEWQDHVEQFADGSSILLRKPKLRFIDLAFGPLGPDVMTSVRIAPPVLGTGLLDAV
                     SEDMLLEIARGQRQVGFGGRLNYVWDAEKQSVAVGRFGWKANQPNLKQQTASAFLNDL
                     GVTNGLFKLENCPPIQIACRKRPRGMVPEQPQRAFEELLFYLRALAAPAPRNLDDPAV
                     QRGERLFTQSKCAICHVPEMKTGDYPALVQLGQQRIRPYTDLLLHDMGEELADGRPDY
                     LAGPRDWRTPPLWGIGLSGTVNGNAALLHDGRARTVAEAVLWHGGEAGVAREAFRGMP
                     AADRAALLAFVASL"
     gene            complement(4460..4669)
                     /locus_tag="VN496_09115"
     CDS             complement(4460..4669)
                     /locus_tag="VN496_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17153.1"
                     /translation="MRACIELLLAVLACTPACAEFVLIPENSTGPPGGVLEVTLPVQN
                     ESSAEELDVQRPARIVLHAHSSASP"
     gene            complement(4737..>4958)
                     /locus_tag="VN496_09120"
     CDS             complement(4737..>4958)
                     /locus_tag="VN496_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXF17154.1"
                     /translation="AGIFAAVASVALVSQAVTFAAGVFAGSMLWWALLTVVVSRSAAM
                     FRPPVMVWINRVSGAILVSFALYGIMQVL"
BASE COUNT          978 a         1504 c         1472 g         1004 t
ORIGIN      
        1 gttcgatggt gcggcgatat tcgagggcat ccgggctgca attgaaatcg ccggtcagga
       61 cggcagagac cggggttgga cgcatctgga aagggccttg cgcggacttg gctttttgcg
      121 cctctgcgcc gcgctggcaa gcctgctgat gcaatgcacg cagggcatcg acttgcgcca
      181 tgcgctgcgt ggcggagtag tactccagat gcgtagtggt gatcctgatg ggcccccacg
      241 gggccgcgac taccgcctcc agggcgatac gttgcatgct gctgtccgcg gcgggatccg
      301 ccggccaggg cagcagatgc cggtacacct gcagcactgg caagcgcgag aacaagacgt
      361 tgccgaacag gctgcggcct ccttgcccat cgtctatgtc cgtggcaacg ccataatgcg
      421 ctgagtaacc gggcaggccc agtgaaagtt cgcgtacctg atcctccccg cgactgccgg
      481 gcagtccggg aaaatttgcc gcgacttcct gcaggcacag cacatcaaag tccgccagtt
      541 tgttagctgt gcgcacgatg cgcgccggat cgactgccag gtcgatgccg cgcgcccact
      601 ggatattcca gctaacgagt ttcatttcag cgcgtgctag taacgggcgt cttccggttt
      661 tccaccttgc gcccagtctg cagacttgcc ggcgaaatcc ggccgcggct ggcgcgtaaa
      721 gaaggcggcg acgtcaaagg cttcttcatc gctgagggag ccgggctggt cgaaaggcat
      781 gttggctttg acaaaagctg ctgcggtatt cagacgcgcc atcccggcgc cgatgttgaa
      841 tgatttcggt ccccacagcg gcggatatag catgtcaccc gaaggggctg gcataccttg
      901 accttgttcg ccgtggcaca cggcgcactt gtgcgcataa atgcgcgcac cgcgttcgcg
      961 atcgggaggc tgcgtgcttt tcatgcgcag gaaaccgcgg ccggtcacgc tttgtcctgt
     1021 cggtacatcg cgcgagagcc actgcatata ggccaggatg ccaatcattt cggcgctggc
     1081 gggcggcagt gccttgccgt tcatggagcg ctcgaagcaa tcgttgatac ggtcctgcag
     1141 cgagatcacc cttgcgctac ggctgcgata ttcaggaaat acgccccaaa tgccgaccca
     1201 gggcgaagac cacggcgtgc ggccggcatt cagatggcaa ctcgtgcaat taagcgcatt
     1261 tccggaatag gcgggagcgc tttgcgccgt gttcgtcagc acgcgccgcc ctagcgcgat
     1321 cgcgtctccc accgggcctt gcggatctgg cgtgtcatgc cagccgggat aaacagtgtc
     1381 ggcagccagc gccacggaaa tacagaatcc caaaccgact gcaagagcaa tgcaggtact
     1441 caaaccacca ggcagcgctc gtcccgcagg cacgttcaat tccggcatgg tgaactacct
     1501 tctttcagtg agcgccaaaa acagaatcta tttcgatgaa gataccgaaa caaaaacccc
     1561 gcttgcgcgg ggttcagagt caagcacgag ccgatggttc aaacgggtag cggatcttgt
     1621 tcgtaagccg gttctttctg tggttcaggg gttggcgcgt tacgttgctt ttccgcttgc
     1681 ctgcgtttgt cttcctcttc ggccagtttt gcccagtaca aggcctcgaa ctcgaagcac
     1741 atgctgcacc tccttccaga tcagttgggg gtagaacgca ttagtgcttt tgttgtgtct
     1801 ttatcgtagc gccgtcaatt catggaatca atcggtttga attgcattca ttgcggcatc
     1861 gcagcaagat gctcacggac tttttccaac gcttcaacca tgatttcggg cggttgtgca
     1921 ccggacaacg ccaggcgctg gttgaagatg aaaaaaggca caccttgcac gccggtggcg
     1981 cgcgcccgcc ggtcctgttc ttctatcaac gcacggtctt cgtcacttgc cagataagtc
     2041 gaggcatcat ctcgattgat gccggcttgc gcagcgatgt ctgcgagttc ggccttatcg
     2101 gtcaggtccg cgccttcctc aaaatatcgg cggaacagcg cttccgccat tgcatcctgc
     2161 ttgccgcgtt ctcccgcgta gtgcatgagc cgatgtgcgt cgacggtgtt gggctgaacc
     2221 tgaatcgcgt cgaacgcgaa ctcgatgcct gcgcgcttgc cggccgttga cacccgcgca
     2281 tagatttcac gggcccgctc cgggccaccg aatttgtttt cgagatattg cttgcgtggc
     2341 acgccgtcag cgggaagttc cgggttgagt tgaaacggat gccagcgcac gatcggcact
     2401 tcatcgggat acctcgctcg ccactggtcg agcgcgcgtt ccaggttgcg tttgccgata
     2461 aaacaccagg ggcacactac gtctgaaacg atatcgattt gaaaagacat gattgctcgc
     2521 ttgtatgtgt tgggtcagtt gcaaaccatg cgcttgacgg ccagcgcagc gttgccgtag
     2581 gagacgcgcc ggtcgaacac ggcattgcag ccgttcatgc cgggcggcag caacgccgtc
     2641 aagctcgcag tggtgcgttc ataagtgcag gccaggtgaa agttgcgttt gctctccgga
     2701 aatcgccagg aaatgcgcag ttctcctttg acggatttga tcgaataagg tttgatccgg
     2761 tgattctgcg atggcggacc atcgtaaatc tgcacgccgg tcaatcgcag gggcgggtcc
     2821 gtatacgaca ccgtccagtc gccggcaatg ttagcctgct gattaacttc aagcgaatcc
     2881 gggcacagca cttcagcgcg ggtggcgcaa cacatcgacg ccagcgcgac agccatcagg
     2941 taacgggtca tgtgaactcc agtcatcagc agtgatcccc ttaaagtgat gccacaaacg
     3001 cgagcaacgc ggcacggtct gctgccggca tgccgcgaaa ggcttcccgc gcaacaccgg
     3061 cctcgccgcc gtgccacaac acggcctcgg ccacggtgcg cgctcggcca tcatgcagga
     3121 gcgcggcgtt gccattgact gtcccgctaa ggccgatgcc ccaaagcggg ggcgtgcgcc
     3181 aatcgcgcgg tcccgcaagg taatccggcc gcccatcggc aagttcttct cccatatcat
     3241 gcaacaacag gtcggtatac gggcgtatgc gctgctgacc caactgcacc agcgctggat
     3301 agtcaccggt cttcatctcg ggcacatgac aaatggcgca tttggattgc gtgaacagcc
     3361 gttctccgcg ctgcacagct ggatcgtcca ggttgcgcgg tgccggtgct gccagcgcgc
     3421 gcagataaaa cagtaattct tcgaacgcac gctgcggttg ttccggcacc atgccgcgcg
     3481 ggcgtttgcg gcaggcaatc tgtatgggcg gacaattctc gagtttgaac agaccatttg
     3541 tgacgccgag gtcattcagg aaggccgaag ccgtctgctg ctttagattg ggctggttgg
     3601 ctttccaacc gaaacgaccg accgcaacgg attgtttctc cgcgtcccag acatagttga
     3661 gccggccgcc gaagccaacc tgtctttgcc cgcgtgcgat ttccagcagc atgtcttcag
     3721 acactgcgtc gagcaggccg gtgccgagaa cgggaggcgc gatgcgtacg gacgtcatga
     3781 cgtccgggcc tagcggaccg aacgccagat cgatgaagcg tagcttcggc ttgcgcaaca
     3841 ggatgctcga tccatcggca aattgttcga cgtgatcctg ccactcaatg actgcatttc
     3901 cttctgccgg cacgcgtccc agttctccct gattctgcag ttgctcacca taattcggat
     3961 gtggcagcgg ggcaccgtgc ggatcttgtc ccggtatcga taaccggacg agcatcgatg
     4021 ccagtgtctc accttccaca tcgggcgaat gaccgcgccc attgttgatg tggcagtcag
     4081 cgcaccgctc tgcattggag gttggtccgc gtccccactt gccgccaatg cccagcagca
     4141 caacccaacg ctgctcgaat tcctgcccgc cgcccgcgaa taattcgagt tgcgcagtgt
     4201 cgagcccgga acccggcagc gagtaggcct gaggtgacgc gtcgtccgtc gtgaacgatt
     4261 cacccgaagt cgcgggctcc cgttgagtga ccagtgccag ggccgcagtc gcgctaaggg
     4321 ccgcacacac gaggatcagc tgccgcagga acagccctgt tttcgcaatc gccatggtga
     4381 gctttctact gccagcgtgt gcggaatgca agggctggcg aaggtgcaag gagccttatg
     4441 gcgcatcacc gcgtaatcat cacggcgatg cggaactgtg agcgtgcagc acgatgcggg
     4501 ccggtcgctg cacgtcgagt tcctcggcgg acgactcgtt ctgcaccggc agcgtaacct
     4561 ccagcacgcc gccgggcggc cccgtgctgt tctcgggaat aagcacaaac tcggcgcacg
     4621 ctggagtgca agccagcacg gcaagcagca attcaatgca cgcgcgcatt actgcagttt
     4681 cttgttcaat ttttgcaaaa gagtccgtcc ttcgcccacc gtaggcattc gctgcgtcac
     4741 aagacctgca tgattccgta gagcgcaaag ctgacgagga tggctcccga gacccgattg
     4801 atccacacca tgactggcgg ccggaacatc gctgccgatc tggacaccac cacggtcagc
     4861 aatgcccacc acagcatcga tccggcgaat acgccggccg cgaaggtcac cgcttgggat
     4921 accaatgcta cggacgccac ggcagcgaag attccggc
//
LOCUS       DATMZQ010000278         5529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_806757, whole genome shotgun sequence.
ACCESSION   DATMZQ010000278 DATMZQ010000000
VERSION     DATMZQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806757"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..76
                     /locus_tag="VN496_09125"
     tRNA            1..76
                     /locus_tag="VN496_09125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Arg,seq:cct)
     gene            321..701
                     /locus_tag="VN496_09130"
     CDS             321..701
                     /locus_tag="VN496_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17155.1"
                     /translation="MNNNNLSDDLIRFIHTSIPGIPFLETLLLLRTEPGRCWTVKAVS
                     GRLYLSTVIADDLLKSLCAAGMAVEGRSGFRYQPQSDELRQMIDRLSDSYRQDLIETT
                     KVIHSGAGKNAQIFADAFKFKKDS"
     gene            703..960
                     /locus_tag="VN496_09135"
     CDS             703..960
                     /locus_tag="VN496_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXF17156.1"
                     /translation="MGSAIYILCMLTSLACAGLLVRSYLATHHRLLFWSGLCFAGLAI
                     SNLMLVLDLMVFPDIDMSSWRLAISTGATALLVFGLIWEEK"
     gene            968..1234
                     /locus_tag="VN496_09140"
     CDS             968..1234
                     /locus_tag="VN496_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXF17157.1"
                     /translation="MTAILVGTVGTLSIVAGLFFLRFWRSTRDRFFLFFALAFFLEGA
                     NRIALYSSVGEQEHLPIYYLIRLASYGLILMAIIGKNWVRRPRV"
     gene            complement(1390..4089)
                     /locus_tag="VN496_09145"
     CDS             complement(1390..4089)
                     /locus_tag="VN496_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXF17158.1"
                     /translation="MSLRSGLSAYGVSLAAVAAAIVVRLLFDPWMGDRFPFITVFLAV
                     PFAAWYGGRGPGLLAMAGGAFGVAFFVLQPRYTLPIGQIEYQVGLVLYAIVCFASIAM
                     FESVRRNRQRAERKQRELEQEMAERRVAEHALAEQEEFMRTTLSSIGDAVLATEARGM
                     VTYMNPVAEALTGWTLAEARGQSFDVVFRIVNEDTLQPVESPVPRALRDGVIVGLANH
                     AILLSKNGKRVPIDDSAAPIRDENGNIRGAVLTFRQVFEQRRMMREREQALATLNNLV
                     ATAPIGITILDKDMRFTHVNAALADMNGVPAEDHIGKTVAEIVPDIYPQVEPIFRRLM
                     DTGEFVPDQILEGETKSNPGVKRVWRESWFPITGPGEDKPVGVGVTVQEITEERRAVR
                     EMAGLVQRLTSLVDNTPLALIEWDTDLVITRWSALAERVFGWTAAEVIGKRFDAFPLV
                     HAEDMEKVQGAIARLLESGSAFVVSRNRNNTKRGAVIHCEWYNSVLRDEAGQMIAVLS
                     LVLDVTERRLAGQALKEADRRKDEFLATLAHELRNPLAPLGNGLQLMRLAGGERATIE
                     QALTMMERQFAQLVRLVDDLMDLSRISNGRIELRKERVQLAAVIASALETSRPLVEEM
                     GHDLAVALPPQPIWLDGDLTRLAQVFLNLLTNAAKYTDRGGHIWLTAEQQGSEVVVSV
                     RDTGIGIPADQIPRLFQMFAQGERSLAKAQGGLGIGLNLVKQLVTMHGGSVEAVSDGL
                     GKGSEFVVRLPIAVEDAVQQSAAELSGLPTTGSAMRILIVDDNRDGADSLSKMLQLMG
                     NDTRTAYDGQEAVDAAGNFRPDVVLLDIGMPQLNGYDACRRIRGQPWAENIVICAMTG
                     WGQEEDRRRSHDAGFDHHMVKPVEPRALMNMLASLPRTSEPSG"
     gene            complement(4189..5274)
                     /locus_tag="VN496_09150"
     CDS             complement(4189..5274)
                     /locus_tag="VN496_09150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXF17159.1"
                     /translation="MVTSEHLLQQLLKESEHAIILLDADGVIVGWHGSAGAIFGYTSE
                     EVLGQRARILFTSEDQTRGVPEHELQEARNSNWSEDDRWMARKGGALFWAAGTTSALH
                     DDEGTIIGFGKVLRERTDVRQQISTLDNRVKALNSFIAVLGHEIRNPLHTISMSMTAL
                     TKLVSGTAAELAGMACRQTAFMTRLVEDLQDATRVTAGKLKLRRERVTVQEVLKKAAE
                     NVRPYALERKQNFQELLLPSPLVVDVDPDRMYQAVCNLLENAIKYTPEGGNIYLKCTV
                     EEAEAVVRVEDDGVGIDQDALPHIFELFTQGRTPEANSQELGAGLGLGLALVKDLVTL
                     QGGTVQVRSEGRNKGAEFIVRLPLAAQ"
BASE COUNT         1117 a         1690 c         1508 g         1214 t
ORIGIN      
        1 gccccggtag ctcagtggat agagcaaccc cctcctaagg ggtaggtcgc acgttcgatt
       61 cgtgttcggg gcgccaacac tcgatgattt agcgacttcg gaggcgccaa accttccata
      121 cccacccgca caccgccatt gcacgtaggt ggtgctcgga gaccttgacc gaaaaaaacg
      181 tcagtgccag ccggtctgtg cggtagcgca caatgcttta gcctgaaatt gagtacaaat
      241 atgccaggtg tataggcaca cgaggtttca ttgcggtttg tcgttgtaga cggcctggcc
      301 ccgatgaacg agtaacaggt atgaataaca ataatctatc tgacgacctg atccgcttca
      361 tccatacgag cattcccgga atccctttcc tggaaaccct tcttctttta agaacggagc
      421 ccggaagatg ctggactgtc aaggcggtat ccggccgtct ctatctgtcg accgtcattg
      481 cagacgacct gcttaagtca ttgtgtgccg cgggcatggc agttgagggg cgttcgggat
      541 tccgttatca acctcaatca gacgaactgc gccagatgat cgaccggctc tcggattcct
      601 acagacagga ccttattgaa accaccaagg tcattcattc cggagctggc aagaatgcgc
      661 aaatttttgc cgacgcattc aaattcaaaa aggattcgta gcatgggatc ggctatatat
      721 atcttgtgca tgttgaccag cctggcttgc gcgggcctgc ttgtgcgcag ttaccttgcc
      781 acgcaccacc gcctgctatt ctggagcggc ttgtgttttg ctggtctcgc aattagtaat
      841 ttgatgttgg tcctggattt gatggttttc cccgacatcg atatgtcttc atggagactg
      901 gcaatctcga cgggagcaac ggccttgctg gtgttcgggc tcatctggga agagaagtga
      961 ggcgaccatg actgcaattc tggtgggcac agtgggaacg ctgtctattg ttgcgggatt
     1021 gttcttcttg cgcttctgga gaagtacacg cgatcgcttt tttctgttct tcgcccttgc
     1081 tttctttctc gagggcgcaa accgaattgc tttgtattct tccgtgggcg aacaggaaca
     1141 tttgcccatc tactatctga taagacttgc aagttacggt ctgatactta tggcgattat
     1201 cggcaagaat tgggtccgcc gcccccgcgt atagaggtat tcccaccgcg ccagcctgta
     1261 atcaacgcgg gctttacatg gcgctggtaa agctggaggc aaggttgatt gctatcaggc
     1321 aatcattcag cggagtaacc gacgtcataa cagcgaggtc atgaccgatt cagcagcaaa
     1381 acgccgtgct cagccagacg gttcgctggt gcgcgggagc gatgcgagca tattcatcag
     1441 cgccctgggt tccacgggtt tgaccatgtg atgatcgaag cccgcgtcgt gcgagcgacg
     1501 gcgatcctct tcctgtcccc atccagtcat ggcgcagatc acgatattct ccgcccacgg
     1561 ctgtccacgg atccggcggc acgcgtcgta gccgttgagt tgtggcatgc cgatgtccag
     1621 caagactacg tccggtcgaa aattccctgc ggcatcgacc gcctcctgac cgtcgtaagc
     1681 cgtgcgcgta tcgttgccca tgagttgcag catcttgctc aggctgtcgg ccccatcccg
     1741 attgtcatcc acgatcagaa tgcgcattgc cgaaccggtc gtgggtaagc cggataattc
     1801 cgctgccgat tgctgaacgg catcttccac cgcgatcggc agccgcacaa caaactcgga
     1861 acctttgccg agcccatcgc tcacagcctc cacgctcccg ccgtgcatcg tcaccagttg
     1921 tttgaccaga ttcagtccga tgcctaagcc cccttgcgcc tttgccagcg atcgctcgcc
     1981 ctgagcgaac atttgaaaca gtcgtggaat ctgatctgcg ggaatgccaa tgccagtgtc
     2041 ccggaccgac accacaacct cactgccttg ctgctcggcg gtcagccaaa tgtgcccacc
     2101 gcgatctgta tacttcgccg cgttggtgag caagttcagg aacacttgag ccagacgcgt
     2161 gaggtcgcca tcaagccaga tcggctgcgg cggcaaggcg acggccaagt catgtcccat
     2221 ctcctccaca agcgggcggc tcgtttcgag cgcgcttgcg ataacggccg cgagttgcac
     2281 gcgttccttg cgcagttcga ttctgccgtt gctgatgcgg ctcaagtcca tcagatcatc
     2341 caccagtctc accagctgcg cgaactgccg ctccatcatg gtcagtgcct gctcgatcgt
     2401 cgctcgctcg ccgcccgcca agcgcatgag ttgcaagccg ttgcccagcg gagccaacgg
     2461 gtttctcaac tcgtgagcca gcgtggccag gaattcatcc ttgcgacggt ccgcctcttt
     2521 caatgcctgc ccagccagcc gcctttcggt gacatcgagc accagggaaa gcacggcgat
     2581 catctggcct gcctcatcgc gcaacactga gttgtaccac tcgcaatgga ttacggcgcc
     2641 gcgcttggta ttgtttctgt tcctcgacac gacaaacgca ctgcctgact cgagcaagcg
     2701 cgcaattgct ccctgaactt tttccatgtc ttctgcgtga acgagcggga atgcgtcgaa
     2761 ccgcttgccg atgacttcag cggcggtcca gccgaacacg cgttcggcta acgccgacca
     2821 acgcgttatg accaagtctg tgtcccactc gatcaacgcc aacggcgtgt tatcgaccag
     2881 ggaggtcagg cgctgcacca agcctgccat ttcgcgtacg gcacggcgtt cctcagtaat
     2941 ctcctgaact gtgaccccga caccaacagg cttgtcttcg ccgggtcccg tgatcgggaa
     3001 ccagctttcg cgccatacgc gttttacccc cgggttgctt ttagtctcgc cctccaaaat
     3061 ctggtcgggc acaaattcgc cggtatccat cagccggcgg aagattggct caacttgcgg
     3121 gtagatgtcc ggcacgattt ccgcgaccgt cttgccgatg tggtcctctg ccgggacgcc
     3181 gttcatgtcg gccagcgctg cgttgacatg cgtaaaacgc atatctttgt ccagtatggt
     3241 gatgcctatc ggtgcggtcg caacgagatt attcagggtt gctaaagcct gctcgcgttc
     3301 ccgcatcatg cgccgctgtt cgaaaacctg gcgaaaggtc agcaccgcgc ccctgatgtt
     3361 tccgttctca tcccggattg gcgccgcact gtcatcgatg ggcacgcgct tgccattctt
     3421 tgacagcagg atggcatggt ttgccaaacc gacaatgacg ccatctctga gtgcgcgcgg
     3481 gaccgggctc tcgaccggct gaagcgtatc ttcattgaca atgcggaaca ccacgtcgaa
     3541 agattgacca cgcgcctccg ccagtgtcca gccagtgagc gcttcagcta ccgggttcat
     3601 ataggtcacc atgccgcgtg cttctgtcgc aagcaccgca tcgccgatgc tggaaagcgt
     3661 ggtgcgcatg aactcttctt gttcggccag agcatgctcg gccacccggc gctcggccat
     3721 ttcctgttcc agttcccgct gcttgcgttc ggcgcgctgg cggtttcgcc gcaccgattc
     3781 gaacatcgcg atggaagcaa aacaaacgat ggcatagaga acgagaccga cctggtattc
     3841 gatctgacca atgggcaacg tatagcgcgg ctgcagcaca aagaaagcaa ccccgaaagc
     3901 cccgcccgcc attgccagca gccccgggcc cctgccgcca taccaggctg cgaagggaac
     3961 ggcgagaaag accgtgatga acggaaagcg gtcgcccatc cacggatcga acagcaggcg
     4021 aacgacaatg gctgcagcga ccgcagcgag tgaaacgcca taagcagata acccgcttcg
     4081 gagactcatg tcgtgaatag cccggcaggg gttgatgaag ggacgggacg cctgcgatca
     4141 atatgaccct gatgccggct ttgcgcaacg cctcgtggac tggcgcggtt attgtgccgc
     4201 cagcggcagc cgcacaatga actctgcgcc cttgttgcgg ccctcgctgc gtacctgcac
     4261 cgtgcctccc tgaagcgtga ccaaatcttt gaccagcgca agccccagtc ccaatcctgc
     4321 ccccagttcc tgggaattgg cttccggcgt cctgccctgc gtgaacaatt caaagatatg
     4381 cggcagcgcg tcctggtcga tgccgacacc gtcatcctcc acccggacga cggcttcggc
     4441 ttcctcgacc gtgcatttca aataaatatt gccgccctcg ggcgtgtatt taatggcatt
     4501 ttccagaaga ttacacaccg cctggtacat gcggtcggga tctacatcca ccacgagcgg
     4561 agaaggcagc aacagctcct ggaagttttg cttgcgttcg agtgcatacg ggcggacgtt
     4621 ttcggcggcc tttttgagaa cctcctgaac tgttacgcgc tcgcgccgca gtttcagttt
     4681 cccggcggta acgcgagtcg catcctgcaa atcttccacc agccgggtca tgaacgcagt
     4741 ctgccggcat gccatgccgg ccagctcggc tgccgtgccg ctcacgagtt tggtgagcgc
     4801 ggtcatgctc atgctgatgg tatgaagcgg attcctgatt tcatgaccca gcacggcaat
     4861 gaaggaattc agcgctttga cgcgattgtc cagcgtactg atctgctggc ggacatccgt
     4921 gcgttcgcgc aggaccttgc cgaagccgat gatcgtgcct tcatcgtcgt gcagggcgct
     4981 ggtggttccg gctgcccaga acaatgcgcc gcctttgcgc gccatccagc ggtcgtcctc
     5041 cgaccagttg ctgttgcgcg cctcctgcaa ttcgtgctca gggacgccac gcgtctggtc
     5101 ctcggacgta aaaagaatgc gcgcacgctg acccaggact tcctcgctcg tataaccaaa
     5161 gatggccccg gcactgccat gccagcccac gattacccca tcggcgtcca acaggataat
     5221 ggcgtgctcg ctctccttca gcaattgctg cagcaagtgt tcgctggtga ccataagaag
     5281 tcgggccggt cgttaacccc tctttaggca ggacaacggg ctctgctttc acgccattgc
     5341 caaactcgct tcataagatc agaggctact gctattggag atacggcgaa cccagaaatt
     5401 ccacagcaga gcgaagatcg tgctcgtcca tcgcgcaata ccgaattcgg actaaaaccg
     5461 aagttgacta caaggcgtac cgcccttgct tgagagcgct ctcaggcagt ccgcgaacgt
     5521 ccgaggtga
//
LOCUS       DATMZQ010000279         3423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1267381, whole genome shotgun sequence.
ACCESSION   DATMZQ010000279 DATMZQ010000000
VERSION     DATMZQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267381"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..660)
                     /gene="rpiA"
                     /locus_tag="VN496_09155"
     CDS             complement(1..660)
                     /gene="rpiA"
                     /locus_tag="VN496_09155"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HXF17160.1"
                     /translation="MDQDQLKQAVAKAAIDHIPDNCVVGVGTGSTANYFIDELAGIKG
                     RIEGAVASSDASAARLRQTGIRVFDLNVVPEMPVYVDGADEINRQLQMIKGGGGALTR
                     EKIVAAVARTFVCIADASKLKDVLGAFALPVEVIPMARSHVGRELVRLGGQPKLRQGF
                     VTDNGNMILDVYGLSIVDAPELETQINQIVGTVTNGLFALRAADILLLGTQSGVQTMR
                     V"
     gene            793..2304
                     /gene="ilvA"
                     /locus_tag="VN496_09160"
     CDS             793..2304
                     /gene="ilvA"
                     /locus_tag="VN496_09160"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HXF17161.1"
                     /translation="MKTDYLEKILNARVYDVAVESPLEIATALSKRLGNDLLLKREDM
                     QAVFSFKLRGAYNKMTHLKASQLAKGIIAASAGNHAQGVALAAQRLDCCATIVMPSTT
                     PRIKVAAVAGRGARVVLFGDSYDDAYAHAMQIAGREKLSFVHPYDDPDVIAGQGTIGM
                     EILRQHAGPIAAIFVPVGGGGLISGIGAYVKRLRPDIRLIGVEPVDADAMARSLAAGR
                     RIRLEHAGLFADGVAVKQVGAETFRLCRELVDEMILVDNDAICAAIKDVFEDTRSILE
                     PAGALAIAGAKAWVERGKVTGRTLVAVASGANINFERLRFVAERAELGEQREAILAVT
                     IPERPGSFRQFCTLLGPRNVTEFNYRYADARQAHVFVGVQVRDRDEIRALVARLRKHK
                     LPTEDLSDNEMAKLHVRHLVGGRAPSVTNELLYRFEFPERPGALMNFLENMSHDWNIT
                     LFHYRNHGADFGRVLIGMQVPPRDKSAFRRFLDHLGYSSCEETRNPARSLFLS"
     gene            2386..>3423
                     /locus_tag="VN496_09165"
     CDS             2386..>3423
                     /locus_tag="VN496_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17162.1"
                     /translation="MTSAKTAFFLIFISCAAIAAPQDDLFLEAREAFQRGDILKLNER
                     ASKLDDDYPLTPYVRYWQLRSHLADLSSAEIQAFLAKHESSLVADRMRADWLRQLARN
                     AEWESFLREYGKLGTADTELACYASQAKLARGDSGVLSQARPLWFNAQALPDSCLPIF
                     QAMFDKEILTAEDSWSRVRLALENGNLANAKLLLMYLPLAQRLDVKLLESTAKRPQRY
                     LDKVPLLLKTRGQRELAIYAVYKTAENWPEIASDHLRSFADKLADEERAYAWAQLASV
                     AARNLHTGALTWFENAGSGMSDRQWSSYARAALRAGEWAGVLTAVNSMSNVERSRPQW
                     RYWRARALMALG"
BASE COUNT          707 a          991 c         1000 g          725 t
ORIGIN      
        1 tcacacacgc atagtctgta cgccgctctg cgtgccaagc agcagaatgt cagccgcgcg
       61 cagcgcaaac aggccgttgg tcaccgtccc gacgatttga ttgatctgcg tctccaattc
      121 gggtgcatcg acaatgctaa gcccgtacac atccagaatc atgttgccat tgtccgtcac
      181 gaatccctgg cgaagcttcg gttgaccgcc cagacgcacc aattcgcggc cgacatggga
      241 acgggccatt ggaatgactt caaccggcag tgcaaaagcc cccagtacgt ccttcagctt
      301 gcttgcatcc gcaatgcaca caaaagtccg cgccacagcg gccacgattt tctcccgggt
      361 tagcgcacct ccaccgcctt tgatcatctg caactggcga ttgatttcat cggcgccatc
      421 cacatagacc ggcatttccg ggacaacgtt gagatcaaat acgcggatgc cagtctgcct
      481 aaggcgtgct gcactggcat ctgaacttgc caccgcgcct tcgatgcggc ccttgatccc
      541 agccaattca tcgatgaaat agtttgctgt ggatccggta ccgaccccca caacgcaatt
      601 gtccggtata tggtcgattg ccgccttggc cactgcttgc ttgagttggt cctggtccat
      661 gcttgccaat cgtaatgtcg ccgaagcata gcctctacgc gctcgcggta caaacgctgc
      721 gtcggaagtt gcatcgacgg gccgcccgct gataggctcg atcggttgcc cgattgataa
      781 ggggaaagct cattgaagac agattacctt gaaaaaatac tgaatgcgcg cgtctacgac
      841 gtagccgttg aatcgccgct ggagattgcg acggccttgt ccaaacgact cggcaatgat
      901 ttgctgctca agcgagaaga catgcaggcg gtgttttcat tcaagttgcg gggtgcgtac
      961 aacaagatga cgcatctgaa agcttctcaa ttggcaaaag gaatcattgc ggcgtctgcc
     1021 ggcaatcatg cgcagggcgt tgctctggca gcccagcggc tggattgctg cgcgaccatt
     1081 gtaatgccgt cgacgactcc acggatcaag gtagccgccg tggcagggcg cggtgcgcgc
     1141 gtggtgttgt tcggcgattc ctatgacgac gcgtatgcgc atgcaatgca aattgccggc
     1201 cgtgaaaaac tcagcttcgt ccatccttat gatgatccgg atgtgattgc gggacaggga
     1261 acaattggca tggaaatcct gcgccagcat gccggtccaa tcgccgcgat ctttgtcccc
     1321 gtcggagggg gaggcctgat ctcgggtatt ggcgcttacg tcaagcgatt gcggcccgac
     1381 atcaggctga tcggcgtgga gccggtcgac gccgatgcaa tggcgcgttc gctcgcggcg
     1441 ggtagacgta tcagactcga acatgctggg cttttcgcgg acggcgtcgc ggtcaagcaa
     1501 gtcggcgcag aaaccttcag gctgtgccgt gaattggtgg acgaaatgat tctggtggac
     1561 aacgatgcca tctgcgctgc aatcaaggac gtgttcgaag atacgcgatc gattctcgaa
     1621 cccgccgggg ctttagcgat cgcgggggcc aaggcctggg tggagcgcgg caaggtgacg
     1681 ggtcgcacgc tggtcgccgt cgcaagcggt gccaacatca actttgagcg cttgcgcttc
     1741 gttgccgaac gcgctgaact gggagagcag cgcgaggcca tcctcgcagt cacgatcccg
     1801 gaacggccgg gcagtttccg gcagttttgc acgctgcttg ggccccgcaa cgtgactgaa
     1861 ttcaattacc gatatgccga tgcgcgccag gcgcacgtct ttgtcggcgt gcaagtgcgc
     1921 gatcgcgacg agatccgtgc cctggtggcg cgattgcgca agcataagtt gcctaccgag
     1981 gacctcagcg acaacgaaat ggccaaattg catgttcgcc acctggtggg cggccgcgcg
     2041 ccttcggtaa cgaatgaatt gctgtaccgc ttcgaatttc ccgagcggcc gggtgcgctg
     2101 atgaattttc tggagaacat gagccacgac tggaacatca ccctattcca ctaccgcaat
     2161 cacggcgccg atttcggccg ggtgctgatt ggcatgcaag tgcctccgcg cgataaatcc
     2221 gcattccggc ggtttttgga ccaccttggt tattcgagct gcgaagaaac ccgcaatcct
     2281 gcgcgttccc tgtttctttc ctgagcatgc cgcggttatg caccagcttg ccacgcggcg
     2341 cgcttataat gatgacatcg acttttttgc cgtttggccc ttccagtgac gagtgcaaaa
     2401 accgcttttt ttctgatttt tatatcctgc gcagcaattg ccgcccccca ggacgatctc
     2461 ttcctagaag cgcgggaggc attccagagg ggcgatatcc tcaagctaaa tgaacgtgcg
     2521 tccaagctgg acgatgacta cccactgact ccgtatgtgc gctactggca gctgcgttcg
     2581 catctcgcag acttatcgtc cgctgaaatc caggcgtttc tcgcaaagca tgaaagcagc
     2641 ctggtggccg atcgtatgcg tgccgactgg ttacgccagc tggcgagaaa cgctgaatgg
     2701 gaaagcttct tgcgcgaata tggcaagctc ggcacggcgg atactgaact cgcctgctat
     2761 gcctcacaag ccaaattggc gcgcggagac tccggcgtac tgagccaggc gcggccattg
     2821 tggttcaatg cccaggcctt accggacagt tgtctgccga ttttccaggc gatgttcgat
     2881 aaggaaattc ttacggccga agacagctgg agccgcgtgc ggcttgcgct ggagaatgga
     2941 aatctggcca acgcgaaatt gctgttgatg tacctgccgt tggcgcagcg gctggacgtc
     3001 aagctgctgg aatcgacagc aaaacgtccg caacgctatc tggataaggt accgctactg
     3061 ctgaaaaccc ggggtcagcg agaactggcg atttacgccg tttacaagac cgctgaaaac
     3121 tggccggaaa ttgcgtccga ccacctgcgc agcttcgctg acaaacttgc cgacgaagaa
     3181 cgggcatatg catgggcgca actcgcctct gtggctgcgc gcaacctgca caccggcgcg
     3241 ctgacctggt tcgagaacgc cggcagtggc atgagtgacc gtcagtggtc ctcgtacgct
     3301 cgcgccgcgc tccgcgccgg cgagtgggcc ggtgtgctca ccgcggttaa cagcatgtcc
     3361 aatgtcgaac gatcacgtcc acagtggcgt tattggcgcg caagagcctt gatggcgctt
     3421 gga
//
LOCUS       DATMZQ010000280         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_807995, whole genome shotgun sequence.
ACCESSION   DATMZQ010000280 DATMZQ010000000
VERSION     DATMZQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807995"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VN496_09170"
     CDS             <1..340
                     /locus_tag="VN496_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HXF17163.1"
                     /translation="VRLEDGTVLALPAARAQKLGNRRDEAVILGLRPEHLNRAQPGDS
                     RTGLARHTATIELLQPTGSRTYATFTLGGSSVVAELQAHDVTQMDQKIELSIDMNRAV
                     LIDPGTENVL"
     gene            371..2338
                     /locus_tag="VN496_09175"
     CDS             371..2338
                     /locus_tag="VN496_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17164.1"
                     /translation="MKIEQPPRGIALCGTPEPDTAGRVLLAGPLSVEFSNGQLRYLKI
                     NGVEVLRAIGFLIRDKNWGTATPAIENLNIEQRRDSFSVSFHASITIEHQALSYDATI
                     EASANGELDFTAVAVPKTDFLTARTGFVVLHPLKGVAGQPVEIEHVDGKKVEGKFPAM
                     VNPIQPVLDIRSLTHEITPGLKFTVRMEGDTFEMEDHRNWSDASFKTYVRPLSRPWPY
                     TLKAGEPVKQAIRITLSGSAAKRRTGGDSKAIDVAIGAVSQKALQPIGLGMPAEEIEP
                     AIAQLALLKLASPRFLICHIDPRSRHGLKELYGYRVLAEESKAGVVLEAVIESVESFR
                     QELTGLAAMVRQSGIKLASLAIVPAGDLKSVLPGGPRPPAPKLEELYAAARAAFPDVR
                     LGGGMFSFFTELNRKRPPAKLLDFVVNSTCPIVHAADDRSVMETLEALPYQVETARSF
                     IGKAAYRIGPSGIGCRDNPHGATWTPNPDNVRVCLTKLEPRQRGLFAAAWSLGYVATL
                     ARTSVEAIAFGAATGPLGIIYRKADHKQPWYDQLSGPAVFPAFHVVSGLTRGAGQKMV
                     NVESSDAARVQALAYRGKGGTTLWLANLTAGNQTVKLSDLRGTLFGTTLDENSFVMAT
                     TDPQRFQKSWRPMSSTLALTAYAVAIISIND"
     gene            2364..2843
                     /locus_tag="VN496_09180"
     CDS             2364..2843
                     /locus_tag="VN496_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXF17165.1"
                     /translation="MSATPEARPPLPPFTLESAVQKVRLAEDGWNSRDPERVSMVYSS
                     DSQWRNRAEFFSGREAIAQFLRRKWARELDYRLIKELWAFREARIAVRFAYEWHDDSG
                     NWFRSYGNENWEFNAQGLMQKRIASINDLPIREADRKFRWALGRRPDNHPGLSELGL"
BASE COUNT          621 a          842 c          864 g          541 t
ORIGIN      
        1 ggtaaggctg gaggatggca ctgtgctggc gcttccggcg gcacgcgcgc aaaagttggg
       61 gaaccggcgc gacgaggcgg tgatcctggg tctgcggccg gaacacttga atcgcgccca
      121 gcccggtgac tcgcgcacgg gcctggcacg acataccgcc acgattgagt tgctgcagcc
      181 gaccgggtcc aggacctacg cgacattcac attgggcggc agcagcgtcg tagcggagct
      241 gcaagcgcat gacgtcacgc agatggacca gaagattgaa ttgtcgatcg atatgaatcg
      301 cgcggtgttg atcgatcccg gcaccgagaa cgttctttag tccgcaatca cgaagcacac
      361 ggagatgaaa atgaaaatcg aacaaccacc ccgcggcatc gcgctttgcg gcacgcccga
      421 accggatacg gcgggtcgtg tattgcttgc tgggccgctg tctgtcgagt tctccaatgg
      481 ccaattgcgc tacctcaaga tcaacggcgt cgaagtgctg cgcgcgatcg gattcctgat
      541 tcgcgacaaa aattggggaa ctgcgacgcc ggcaattgaa aatctgaaca tcgaacagcg
      601 ccgcgacagc ttttcggtgt cgttccatgc ctccataacg atcgaacacc aggcactctc
      661 ctacgatgca accatagagg catccgcgaa cggcgagctc gatttcaccg cggttgcagt
      721 tccgaaaacc gattttctga ccgcacgcac gggttttgtg gtgctgcatc cgctcaaggg
      781 agtggccggc cagcctgtcg agatcgagca tgtcgatgga aaaaaagtcg agggcaaatt
      841 tcccgcaatg gtcaatccga tccagcccgt gctggacatt cgatcgctga cacacgaaat
      901 tacgcccggc cttaaattca cagtgcggat ggaaggcgac actttcgaaa tggaagatca
      961 tcgcaactgg agcgacgcct cgttcaaaac ctatgtgcgg ccgctttccc ggccgtggcc
     1021 ttatacgctc aaggcggggg aacccgtaaa gcaggcaatc agaattaccc tgagcggtag
     1081 cgcggcaaaa cgtcgcacgg gcggcgacag caaagccatc gacgtagcaa tcggtgcggt
     1141 gtcgcaaaaa gcgttgcagc cgattggact tggcatgccg gcagaagaaa tcgaacctgc
     1201 catcgcacaa cttgcgctgc tcaaactggc ctcgccgcgc tttctgattt gccatatcga
     1261 cccgcgcagc cgccatggat tgaaagaact gtacggctat cgcgtgctgg ctgaagagag
     1321 caaggcggga gtcgttctgg aagcggtcat cgaaagcgtc gagagcttca ggcaggaact
     1381 gacggggctt gctgccatgg tccggcagtc gggcatcaaa ttggcttcgc tggccattgt
     1441 tcccgccggc gacttgaaat ccgtgctgcc cggcggaccg cgtccgccag ccccgaaact
     1501 cgaagaactg tatgccgcgg cgcgcgccgc gtttcccgac gtccggctcg gcggcggcat
     1561 gttttctttt ttcactgaac taaaccgtaa acgccctcct gccaaactgc tcgactttgt
     1621 ggtcaacagc acttgcccaa tcgtgcatgc agccgatgac cgctcggtga tggagacgct
     1681 cgaggcgttg ccgtatcagg ttgaaaccgc gcgttcattc attggcaaag cggcgtaccg
     1741 catcggtccg agcggcattg gatgccggga caatccgcac ggtgcgacct ggacgcccaa
     1801 cccggacaat gtgcgcgtgt gcctgaccaa gctggagccg cgccagcgcg ggctgttcgc
     1861 ggcggcatgg tcgcttggat atgtggcgac gctggcacgc accagtgtcg aagcgatcgc
     1921 gttcggcgcc gcaaccggac cgctcgggat catttatcgc aaggccgatc acaagcaacc
     1981 gtggtacgac cagctttccg gccccgcggt gtttccggca tttcacgtgg tcagcgggtt
     2041 gacgcgcggc gccggacaga aaatggtgaa tgtggaatcc tcggacgccg cacgcgtaca
     2101 ggcgcttgct taccgcggca aaggtggcac aacgctgtgg cttgccaatt tgaccgcggg
     2161 aaaccaaacg gtaaagcttt ccgatctgcg gggcacactg ttcggcacga cactcgatga
     2221 aaacagtttt gtcatggcca cgaccgatcc gcaacggttt cagaaaagct ggcgaccgat
     2281 gtccagcaca ctggctctga ccgcctatgc cgtggcgatt atctcgatca acgactgaag
     2341 ccgtgctctt ctgaccgagg accatgtcag ccactccgga agcgcgccca ccgttgccgc
     2401 cattcacgct tgaaagcgct gttcaaaaag tgcgactggc cgaagacggc tggaattcgc
     2461 gcgatccgga gcgcgtgtcc atggtgtatt ccagcgatag ccagtggcgc aaccgtgcgg
     2521 aatttttttc cgggcgcgag gccatcgcgc agtttctgcg gcgcaaatgg gcgagagaac
     2581 tcgattatcg tttgatcaag gaactgtggg cctttcggga agcccggatc gcggtgcgct
     2641 ttgcctacga atggcatgat gattcgggca actggtttcg ttcatacggc aacgaaaact
     2701 gggaattcaa tgcgcagggc ttgatgcaaa aacgcatcgc gagcattaac gacctgccaa
     2761 tccgggaagc agaccgcaag ttccgctggg cactcggccg ccgtccggac aatcacccag
     2821 gtttgtccga actgggactg tgacgcagcg caagaagccg ccaccgca
//
LOCUS       DATMZQ010000281        12030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2646925, whole genome shotgun sequence.
ACCESSION   DATMZQ010000281 DATMZQ010000000
VERSION     DATMZQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646925"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VN496_09185"
     CDS             <1..566
                     /locus_tag="VN496_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXF17166.1"
                     /translation="GTIDLECGISSNTLSRQKQVDFSLMTWVDGANFLVRDDSPFKTL
                     ADLNGKRIAVIPGTTTVRALTDALSKSYIKAEIFTVHTHVAGLEALSKGNAEAYASDQ
                     TVLIGLALAGAKSIKVRLGDGNLSYEPYGLMMRKNDQDFRQAVNTVLARIYRSGQIAP
                     IYDRWFGKLGKPSQFITMMYLLNGLPE"
     gene            complement(677..1639)
                     /locus_tag="VN496_09190"
     CDS             complement(677..1639)
                     /locus_tag="VN496_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXF17167.1"
                     /translation="MTTILGSGEFRYEVQENWAKLPDGWSFRDVAAVAVDHKDQVYIF
                     NRGEHPMIVFDRDGNFLRSWGEGVFNRAHGLHIGPDETLYCTDDGDHSVRKCTLDGKV
                     LLTIGIPGKPSPYMSGRPFHRCTHTALSPRGDIYVSDGYGNSRIHQYSPDGKPLKSWG
                     ESGTGPGEFNIPHNVCCDQEGWIYVADRENHRVQVFDDQGNYETEWHNLHRPCGMYME
                     TRRNPLCYIGEIGPTMPVNREVPNLGPRISIVDNEGKLVSRFGAAHAGYDRPDQFVAP
                     HGMAVDSRGDIYVGEVSYTAWSQIYPDKDIPAGLPTLRKLVRVR"
     gene            complement(1713..2060)
                     /locus_tag="VN496_09195"
     CDS             complement(1713..2060)
                     /locus_tag="VN496_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17168.1"
                     /translation="MKVMLQHLAHTRSGDKGNTSNIAVIAYAPELYSLLKEQLTEDKF
                     KAHYSGVVTGKVTRYEIDGLQALNFVAEGALGGGVSRSLCLDNYGKALSARLLSFELE
                     VPEGLRSKLRGFV"
     gene            complement(2144..3499)
                     /locus_tag="VN496_09200"
     CDS             complement(2144..3499)
                     /locus_tag="VN496_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXF17169.1"
                     /translation="MKSTVRIGAGSAWWGDRIEPARWNAERGDLDYLCFETMAEATIS
                     AAQVRKRRDPSFPGYDTYLDDRMQAVLPGCLKRGTKIITNQGWINPDGAAERIVYWLK
                     KLGARGIRVAAVNGSLITDRVLQLTSTIMENGRPTATLADTLVSAEAYLGAEPLVDAL
                     RQGAQVVVSGRVADPSLFMAPLMYEFGWDALDHDRLGAGNGLGHLMECGAQVTGGYFS
                     DPGFKDVPEPWNLAFPIVEAQGDGSAVLTKVSGTGGAVNLMTVKEQMLYEVHDPANYI
                     TPDVIVDFTSAQLEQVGPDRVRITHISGKPRTPTLKVSMGCTEGYIGEDMFFYAGPGA
                     LRRAQLAKKILEERFKIVKLDAEEIRIDFLGLNAIHGAMTPRDAPEPYEIAVRVAART
                     KTREEAIKVGREVDGMAVSGIGMTGKRVPHQDRTREIIGVWSSLVPREKVVPSINFFE
                     T"
     gene            3790..4971
                     /locus_tag="VN496_09205"
     CDS             3790..4971
                     /locus_tag="VN496_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17170.1"
                     /translation="MLKRSIARFSRLLALSFVFAASTASAQSPIRIGWLSSLTGPLSS
                     AAIAENQGVQFAVEEINATGGVNGRKLELITRDTSGEPTKAVNLAKQLAFVDKVDFII
                     GPVNSGESLATVPIVAKAGIPNLIIGTIDELVDPQKFPLAFRAINTNKQWIEAANDYA
                     LNVLKKRKVAVIGDTSGYGTASSKLAQDLLDKAGVKPVYVALVDPNKTDLTDEMNKAK
                     AAGAEVIMPWSAATGLLARLFNTRGDMGWDVPIVGHPAMMAPPVRPLLNKPEYWKNAF
                     AAGYVSTTYDAKGKLPPRTQALMDKIRPKLGKGDIDFMFWWIALGYDTVKIVEHAVKT
                     AGSTDAAAIGKVLESTRDFPGAYASYSWTRENRNGYPDHAIIMNVAGSFKDGSFMAAP
                     R"
     gene            5099..5959
                     /locus_tag="VN496_09210"
     CDS             5099..5959
                     /locus_tag="VN496_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17171.1"
                     /translation="MLVALITGLATGALYAAAALTYNVMFSTSKVLSVTTGHITMLGG
                     IFGAWFIATLGWPIPIALLGSIVVGAAFGLLTDWIAIRRVLARSDEHLWLLSTLALAT
                     MVQQAAGLWWGTEPKPFPRLIPQDFSAGLADQKYWLPVAIAILLAIALELFYRRTLYG
                     KLFVAMSEDAFAARARGIPTGRIRSMSYVIAGAMGGLAGFAAGQLTFAYFALGLTLTL
                     NGFIALAVGGLGSNVGALVGGAALGILSAVATYFVGGEYQQTISVGLLMIVLLLKPAG
                     LFGNQSVRPV"
     gene            5956..6963
                     /locus_tag="VN496_09215"
     CDS             5956..6963
                     /locus_tag="VN496_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17172.1"
                     /translation="MKLSHRSWAVAAVIAVLFTLPWWSGDLYKVHLAALICTYWVLIS
                     GLNLVVGYSGQLSIGHVGLLAIGAYSFAILAGTHGMHPAVAIAASGALCALCGLLLGL
                     PSLRLPGFYFAMATLAFALIVTEITLAQGNLTGGGVGLPVPGFPPPFDTPRGSYWLIL
                     AIASVVTWLTWNVARHTWGRGLVAVRDSVVTAQAVGVPVFRAKLTVFVFSGFTAGIAG
                     ALFAVLQSYITPDTFVFDLGLFFFVCIIIGGRGHILGPAIGTIVLTALPEMVAPLAKL
                     GNFFYGLLLLVVVLLVPEGIGRVFQLLLERFRPRRGESHVVKPDLERLSRAIHAGRAI
                     K"
     gene            6960..7712
                     /locus_tag="VN496_09220"
     CDS             6960..7712
                     /locus_tag="VN496_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17173.1"
                     /translation="MSLAARDVTKRFGGLTALSRVSLDVMPGEVHGLIGPNGSGKTTL
                     LNLLSGYYRPDEGAIQMAQQDLSSATVQQRANLGIARTFQKPRLLPTLSVLDNAMIGG
                     WRDARAGFLATAFMLPRASFDERELRNRAEEFLHGVGLSHAISRRANLLEHAEQRFLE
                     IARALTQRPQFMLLDEPAGGLTPAEIEYLGGVIRVIAESGVGVLLVEHHTDFVFRISD
                     RVTTLDVGAVIKHGTPDQVKRDPEVVRVYLGA"
     gene            7714..8457
                     /locus_tag="VN496_09225"
     CDS             7714..8457
                     /locus_tag="VN496_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17174.1"
                     /translation="MRSDRPLLKVENLHVAYGKAEVVHGISLTVRQGEFVVMLGRNGA
                     GKSTTLHAISGLIEKKAGRVEFDGRDLSAASPRDLVRAGVVQVLEGHRVFTTLTVEDN
                     LLIGTYARSAHGDRGKLARIYELFPELREKRNDPASRLSGGQQQILAVAQGVIGEPRL
                     LILDEPSAGLAPLVIERILAVAAELRKGGMAVLLVEQLVEKALRHASYCYLMETGRIA
                     AAGTPKEIQASDVLHQTYLGGHAGTRSGT"
     gene            8475..9530
                     /locus_tag="VN496_09230"
     CDS             8475..9530
                     /locus_tag="VN496_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXF17175.1"
                     /translation="MAATKRWKNRPQGSTWGDFGPDDQLGRLNLLTPEKVKQGIAEVK
                     EGLAFCLSLPLDYPGGNVLNPRRHPPILRPTLRNGRPNMVYIVQRDNPDATDVINDDA
                     VILHLQYSTQWDSLAHVGQLFDADGDGKAEPVFYNGYRAGKEISGPTDPDDAGAIGNV
                     PAKTTTAVHALGVENMAVHCVQGRGVMIDLHAHLGRAGTAVGYDQLMRIMESDKVVVE
                     KGDMVCLHTGFAQMILEMNKQPDLHKLEHSCAALDGRDRKLLQWITDSGLVSLIADNY
                     AVEAHPATSHHGSCASLPLHEHCLFKLGVNLGEIWHLTPLANWLREHKRSRFLLTAPP
                     LRLPGAVGSPATPVATV"
     gene            9549..10310
                     /locus_tag="VN496_09235"
     CDS             9549..10310
                     /locus_tag="VN496_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXF17176.1"
                     /translation="MPTRASAINSVQKSCRILRSLTEPRVTRLSEIAGQAGLNKVTTL
                     RILEVLCREGFVKRDDKTKTYSLGNEVFILAAALRDRDDLRARARPSLVRLAAMSEDS
                     VLLSVRSGGTESVCVDREVGSFPIRANYLDIGSRRPLGMGAGAMALLAWQPQAEIEAI
                     LPLVEQRLPQYPKISLAWLKSEMERSRERGYTVFLNMLVERMGAVGVPIFGSDGSAVA
                     AISIAALSDRLSSRMDQLVGMLKREAEAIGNPKRT"
     gene            10494..11630
                     /locus_tag="VN496_09240"
     CDS             10494..11630
                     /locus_tag="VN496_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXF17177.1"
                     /translation="MSFITGAGLTPYGKQPDKNTLDLMSEAAASALADAQLERADIDG
                     LVCGYSTTMPHLMLSTVFAEHFGLNPAYAHAIQLGGATGFAMAMLAHHLVDSRVADNV
                     LVVAGENRLTGQTRDSAIQTLAQVGHPVYEVPLGATIPAYYGLLASRYMTEYGTTESD
                     LAELAVLMRKHACMHPGAQFRTPITVDAVLESKPIASPLKLLDCCPVSDGGAAFVVRR
                     VPGNGHAVRIRGTGQSHTHQHISAAPSLTQFAAGASVAKALGAAGAELGDVNYAAIYD
                     SFTITLTILLEEIGLAPRGGAGRQAREGHYDHDGPLPLNTHGGLLSYGHCGVGGAMAH
                     LVEAQRQLTNRAQNRQAGNATLALLHGDGGVLSSHVSLVLERVA"
     gene            11627..11980
                     /locus_tag="VN496_09245"
     CDS             11627..11980
                     /locus_tag="VN496_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXF17178.1"
                     /translation="MNPLDWTNDGDGITYQCCRQCDSRWYFQRGFCPRCGSSNPTTLQ
                     ASGIGMVHARTLVSRAPTEEWKAHSPYLIVMIDADEGFRLMAHGSMELQIGTRVRARF
                     IALAGRKIPYFEKFQ"
BASE COUNT         2385 a         3467 c         3629 g         2549 t
ORIGIN      
        1 caggcaccat agacctggaa tgcggcatca gctccaacac gctttcccgt caaaagcagg
       61 tggacttcag cctgatgacc tgggtggatg gcgcaaactt tctggtacgt gacgattctc
      121 ctttcaagac gttggcggat ctcaacggta aaagaattgc agtcataccg ggcaccacca
      181 ccgttcgtgc gcttacggat gcgctctcaa aaagttacat caaagcggaa atcttcacag
      241 ttcataccca tgtcgcgggt ctcgaagcat tgagcaaggg caacgcggag gcttatgctt
      301 ccgatcaaac cgtgctgatc gggctggcgc ttgccggagc aaaatcgatc aaggttaggc
      361 ttggggatgg caatctttcc tatgagccat atggcctgat gatgcgcaag aacgatcagg
      421 atttccggca ggcagtcaac actgtactgg cgcggatata ccgctccgga cagattgctc
      481 cgatctatga tcgctggttt ggcaaactcg gcaaacccag ccagttcatc accatgatgt
      541 acctgttgaa cgggctgccg gagtaagcgg gttccactgc ttgttcgagc ggaagacggc
      601 agagaccgaa ctgggtcagt caacgaatga tccagtgttc ctccgttgtt ccttcccttt
      661 tgcgccgccc tgaggcctag cgaacccgca ccagcttgcg cagcgtcggg agtcccgccg
      721 gaatatcctt gtcgggatag atttgcgacc acgccgtata cgaaacctcg ccgacgtaga
      781 tgtcaccgcg cgaatccacc gccatgccgt gtggcgcgac gaactggtct ggccggtcat
      841 agcctgcgtg cgcggcgccg aagcgcgaca ccaacttgcc ttcgttgtct acgatgctga
      901 tgcgcgggcc aaggttcggg acctcgcggt tgaccggcat ggtagggccg atttctccga
      961 tgtagcacag cggattgcgc ctggtttcca tgtacattcc gcaaggccga tgcaagttgt
     1021 gccactcggt ttcatagttt ccctggtcgt cgaatacctg tacgcgatgg ttctcgcggt
     1081 cggcaacgta aatccagccc tcctggtcac agcacacatt gtgcggaatg ttgaattcgc
     1141 caggaccggt gccagactcg ccccacgact tcagcggttt tccgtctggc gaatattgat
     1201 gaatgcgcga gttgccataa ccgtccgaga cgtaaatgtc gccgcgcggg gagagtgccg
     1261 tgtgcgtgca gcgatggaaa ggtctgccgc tcatataggg cgatggtttg cctgggatgc
     1321 caatcgtgag caataccttg ccgtccagcg tgcacttgcg cacgctatgg tctccatcgt
     1381 ccgtgcagta cagcgtctcg tcaggcccaa tgtgcagtcc gtgcgcacga ttgaatacgc
     1441 cctcacccca ggaacgcaga aaattgccgt cgcgatcgaa tacgatcatg ggatgctcgc
     1501 cccgattgaa gatgtacacc tggtctttgt gatcgacggc gacggcggct acgtcgcgaa
     1561 acgaccagcc gtcgggcagc ttcgcccagt tttcctgcac ctcgtagcgg aattcgccgc
     1621 tgccaagaat tgtagtcatt gacgcatccc tcatgaatta tgtgtcgagt aattggaaag
     1681 aacatgcccc ctggtgagtt tgtttttcat cctcagacga aaccgcgtag cttcgagcgc
     1741 agcccttccg gaacttccag ttcgaacgac agcagcctgg ccgatagcgc cttgccgtag
     1801 ttgtccaggc aaaggctgcg cgatacgccg ccgccgagcg cgccttccgc gacgaagttc
     1861 agcgcttgca atccgtcgat ttcgtaacgt gtgaccttgc cggtaacgac gccggaatag
     1921 tgcgccttga acttgtcctc cgtgagctgt tccttgagca aggaatatag ttccggcgca
     1981 tatgcgatca ctgcaatgtt cgaagtgttg cccttgtcgc cggaacgggt gtgggccagg
     2041 tgttggagca tgaccttcat tgtgagtacc ggcgatgtca tcaatattgt gtaaggctaa
     2101 acgcaagcag aaggctggaa gccattgcgt attaggtccg ccatcaggtt tcaaaaaagt
     2161 tgatcgaggg gacgactttc tccctcggaa cgagcgagga ccacacaccg attatctctc
     2221 gtgtacggtc ctggtgtggc acgcgctttc cggtcatgcc gatgcccgat acggccatgc
     2281 cgtcgacctc gcgccccacc ttgatggctt cttcgcgcgt cttggtgcga gctgccaccc
     2341 gaacggcaat ttcgtagggc tcgggcgcgt ccctcggcgt catggcaccg tggattgcat
     2401 ttagcccgag aaaatcaatg cgtatttcct cggcgtcaag tttgacgatt ttgaaacgtt
     2461 cttcgaggat ttttttggcc agttgcgcgc gccgcagtgc gcccggtccg gcataaaaga
     2521 acatgtcctc gccgatgtag ccctcggtgc agcccatcga caccttcaaa gtcggcgtgc
     2581 gcggtttgcc cgaaatatgt gtgatgcgaa cgcgatccgg gccaacctgc tccagttgcg
     2641 cgctggtgaa gtccacgatg acatccggcg tgatgtaatt tgcagggtcg tggacttcat
     2701 agagcatctg ttccttcacg gtcatcaggt tgaccgcccc gccggtcccg gaaaccttgg
     2761 tcagtacggc agagccgtca ccctgcgcct ccacaatcgg aaaggccagg ttccagggtt
     2821 ccgggacgtc tttgaaaccg ggatccgaaa aatagccgcc ggtgacctgg gcgccgcact
     2881 ccatcaggtg cccgagtccg ttaccggcgc cgaggcggtc atggtccaat gcatcccacc
     2941 caaactcata cattagcggt gccatgaaca gcgaaggatc ggccacgcgg ccggatacca
     3001 cgacctgtgc cccctgtcgg agtgcgtcga ccaggggttc ggcgccgaga taagcctcgg
     3061 cagagaccag ggtgtccgcg agcgtcgcag tcggtctgcc gttttccatg atggtgctgg
     3121 taagttgcaa gacgcgatcg gtaatcaggc tgccattgac tgccgcgacc ctgatacccc
     3181 tcgcgcctag tttcttcagc cagtaaacga tgcgctccgc ggcgccgtcc ggattgatcc
     3241 aaccctggtt ggtgatgatt ttcgtgccgc gcttgaggca acccggcaaa acagcttgca
     3301 tacgatcatc gaggtaagtg tcgtacccgg gaaacgacgg atcacgccgt ttgcgcacct
     3361 gggctgccga gatcgtggct tcggccatgg tttcgaaaca gaggtagtcc aggtcaccgc
     3421 gctcggcatt ccatcgcgcc ggttcaatgc gatcgcccca ccatgctgat ccggcgccga
     3481 tgcggaccgt gcttttcatg gtgcagtttc caatctgctg cagaacatcg ttcccccatt
     3541 tactttcagt ccccattgtg gaacttttgc cggcgcaata ctggagtctc gctgcggtgg
     3601 agtcaatcga cacagattcc ggaaaccgga acggcgatgt agcgaaagcg gcttaatgcc
     3661 ctgatcgccc tgttaaagcg atcagcaccg aattccgcat ctcagaactg atcttgacaa
     3721 agtggctact ggccaacgaa tatttggcga aaaggcgcct tgagcgtccc ttccccgagg
     3781 agaaaatcaa tgttgaagcg ctcgatcgcg cgtttttcgc ggctgctggc attgtcattc
     3841 gtgttcgctg cgtctactgc atcggcccag tccccaattc ggatcggatg gctttcttca
     3901 ctgaccggac cgttatcctc cgcggccata gcggaaaatc agggtgtgca gttcgccgtt
     3961 gaagaaatca acgccaccgg tggagtcaat ggccgcaagt tggaactcat tacgcgcgac
     4021 accagcgggg agccgaccaa ggcagtgaac ttggccaagc agctcgcatt cgtcgacaag
     4081 gtcgatttca tcatcggccc ggttaattcc ggcgaatcgc tcgctactgt gccaatcgtc
     4141 gcgaaggctg gcattcccaa tctcatcatt ggcaccatcg atgaattggt cgatccgcag
     4201 aagtttccgc tggcatttcg tgcgatcaac accaacaagc aatggatcga agcggcgaac
     4261 gactacgcgc taaatgtgct caaaaaacga aaggtcgcgg tcattggcga cacctccggt
     4321 tacggtaccg cttcttcaaa gctggcgcag gacctgctcg acaaagcggg cgtgaagccg
     4381 gtctacgtcg cgctggtcga tccgaacaag accgacttga ccgacgaaat gaacaaggcc
     4441 aaagcagccg gggcagaagt gatcatgccg tggtccgcgg cgaccggctt gctggcgcgg
     4501 ctgttcaata cgcgcggtga tatgggttgg gatgtgccca tcgtcggcca tcccgccatg
     4561 atggcgccgc cggtccggcc actgctgaac aaaccggagt actggaaaaa tgccttcgca
     4621 gcaggctatg tgagcaccac gtacgatgca aaaggcaaac tgccgccgcg cacgcaggcg
     4681 ctcatggaca agatccggcc gaaactgggg aagggggaca tcgatttcat gttctggtgg
     4741 attgcgctcg gctacgacac ggtgaaaatc gtggagcacg cggtcaagac tgcgggttcc
     4801 accgatgcgg ccgcaatcgg caaagtcctg gagagcacac gtgatttccc cggcgcttat
     4861 gcgagctata gctggaccag ggaaaaccgt aacggctatc ccgatcacgc aatcatcatg
     4921 aacgtcgccg ggagcttcaa ggatggatca ttcatggccg cgccgcgcta gcgcacgttc
     4981 tcgattggct ggtcgaagcc tggacgctgc agttagatgc aaggcatggg tggtaagcac
     5041 tatcgctccg tccacatgaa aaattttttc tgcagaagag ttaactgatg ccgatccgat
     5101 gctagttgcg ctgatcactg gcctcgccac cggcgccttg tatgccgccg cagcgctgac
     5161 ctacaacgtg atgttctcga cttcgaaggt cttgtctgtc accacgggcc atataacgat
     5221 gctcggcggc atcttcggcg cctggttcat tgcaacgctc ggctggccaa tccccattgc
     5281 gttgctgggt tcgatcgtcg tcggtgcagc gtttggactg ctcactgatt ggatcgcgat
     5341 tcgccgcgtt ctggctcgca gcgatgagca cttgtggctg ctgtcaacac ttgcccttgc
     5401 caccatggtg cagcaggccg cgggcctgtg gtggggcacg gagcccaaac cctttccgcg
     5461 cttgatcccg caggattttt cagccgggtt agccgatcag aaatactggt tgccggttgc
     5521 tatcgcgatt ttgctcgcca tcgcgcttga actgttttat cgccgtacgc tatacggaaa
     5581 actgttcgtg gcaatgtcag aggatgcttt cgcggctcgt gcgcgcggca ttccgaccgg
     5641 ccgaatccgc tccatgtcgt atgtaatcgc cggggcaatg ggaggtttgg cgggcttcgc
     5701 cgcggggcag ctgacattcg cttacttcgc gctcggactt acgctgaccc tgaacggttt
     5761 catcgcgctc gccgttggcg gactcgggag taatgtcggc gcgctggtcg gcggggcggc
     5821 gctcggcatc ctgagcgcgg tggcgacgta tttcgtgggt ggtgaatatc agcagaccat
     5881 ttccgtggga ctgctgatga tcgtgctgct gctcaagccg gcggggctgt tcggcaatca
     5941 gagcgtgcgt ccggtatgaa attgtcgcat cgatcctggg ccgttgcggc cgtcatcgcg
     6001 gtactgttca cgctgccgtg gtggtcaggc gatctctaca aggtgcatct ggcggccctc
     6061 atttgcactt actgggttct catcagcggg ctcaatttgg tcgtgggcta ttcgggccag
     6121 ttatccatcg gccatgtcgg tctgcttgcg attggcgctt acagcttcgc gatattggcc
     6181 ggcactcatg gcatgcaccc ggccgttgcg attgctgctt caggggcact gtgcgcgctt
     6241 tgcggactgc tgcttggcct gccctcactg cgtcttccgg gattttattt tgcgatggcg
     6301 acgctggcat ttgccctgat cgtgactgaa atcactctgg cgcagggcaa tctcacgggg
     6361 ggcggcgtcg ggctgccggt gcccggattt ccgccgccat ttgatacgcc tcggggaagt
     6421 tactggttga tccttgccat tgcttcagtg gtgacctggc tgacctggaa tgtggcgcgc
     6481 catacgtggg gacgcggatt ggtcgctgta cgcgatagtg tcgtgaccgc acaagcggtc
     6541 ggggtgccgg tttttcgcgc gaagctgacg gtgtttgttt tcagtgggtt caccgccggg
     6601 atagcgggtg cactgttcgc tgtgctgcaa agctatatta cgcccgatac tttcgtgttc
     6661 gatctgggcc tgttcttttt cgtttgcatc atcataggcg gaaggggcca cattctcggg
     6721 cccgccattg gaaccatcgt gctgaccgcg ttacctgaaa tggtggcacc gctcgcgaaa
     6781 ctcggcaatt tcttctatgg tttgctgctg cttgtggtgg tgttgctggt gccggaaggc
     6841 atcggccgtg tgttccagct gctgctcgaa cgtttcaggc cacgacgagg cgagagccat
     6901 gtagtgaaac ctgatctcga acggctatcc cgggcaatcc acgccggacg ggcaataaaa
     6961 tgagtcttgc agcgcgcgac gtcacgaagc ggtttggcgg gctgacggca ctgtcccgcg
     7021 tatcgctgga tgtgatgcct ggtgaggtgc acgggttgat cgggccgaac ggcagcggaa
     7081 aaacgacgct gttgaatctg ctgtccggct attaccggcc agatgagggc gctattcaaa
     7141 tggcccagca ggatctttcc agtgcaaccg tgcagcaacg tgcaaacctt ggcattgcac
     7201 gcaccttcca aaaaccgcgc ctgctaccaa cgctgtcggt gctcgataac gccatgatcg
     7261 gcggctggcg cgacgcccgc gccggatttc tcgcgactgc attcatgctg cctcgggcga
     7321 gcttcgatga gcgcgaattg cgcaatcgcg cagaagagtt tctacatggc gtcgggttgt
     7381 cgcacgccat ttcccggcga gcgaacctgc tggaacacgc cgagcagcgc tttctcgaaa
     7441 tcgctcgtgc tctgacacaa cggccgcagt tcatgctgct cgatgagcca gcgggtggcc
     7501 tgacgccggc cgagatcgaa tatctcggcg gggtgatcag ggtcatagcc gaatcgggcg
     7561 tgggagtcct attggttgag caccataccg attttgtgtt ccgcatctcg gatcgggtca
     7621 ccacgctgga cgtcggcgcc gtgatcaagc acggtacgcc cgatcaagtg aagcgagatc
     7681 cggaagtcgt ccgcgtatac ctgggggctt gacgtgagaa gcgaccggcc actgctcaaa
     7741 gtcgaaaacc ttcacgttgc ctacggcaaa gccgaagtgg tacacggcat ttccctgacc
     7801 gtacgccagg gagaattcgt cgtgatgctg ggtcgtaacg gagcggggaa gagcacgacc
     7861 ttgcacgcga tttccggctt gatcgaaaag aaggcgggac gcgtcgaatt tgacggccgc
     7921 gacttgagtg ccgccagtcc acgcgatttg gtgcgtgccg gcgtggtcca ggtattggaa
     7981 ggacatcgcg tgtttaccac gctcaccgtg gaagataatt tgctgatcgg gacctacgcg
     8041 cgtagtgcac atggcgaccg tggcaaacta gctcgcattt acgagttgtt ccctgagctg
     8101 agggaaaagc gcaacgatcc ggcttcccgg ctgtcgggcg gccagcagca aattctcgcg
     8161 gttgctcagg gcgtgatagg cgagccgcgg ctcttgattc tcgatgagcc ttccgccggg
     8221 ttggcgccgc tggtgataga acgcatcctg gcggtcgcag ccgaactgcg taaaggcggt
     8281 atggccgttc tgctggtgga acagctggtg gaaaaagcgc tgcgccacgc aagttattgc
     8341 tatctgatgg aaacaggccg catagcggcc gcagggactc cgaaagagat ccaggcgagc
     8401 gatgtactgc atcagactta tctcggcgga catgccggaa cccgaagcgg aacttgaaca
     8461 ggagcacgga gcaaatggcc gcaacgaaac gctggaagaa ccgcccccaa gggtcgacct
     8521 ggggcgattt cgggccggat gaccagctcg gcagactgaa cctgctcacg ccggagaaag
     8581 tgaagcaagg tatcgcagag gtgaaggaag gacttgcgtt ctgcctgagc ctgccactgg
     8641 actatccggg cggcaacgtt ctgaatcctc gacggcatcc gcccattctg cggccgacgt
     8701 tgcgcaacgg acgcccgaat atggtgtaca tcgtgcagcg ggataacccg gatgcgaccg
     8761 atgtaatcaa tgatgatgct gtgatccttc acctgcaata ctccacgcag tgggatagtc
     8821 tcgcgcacgt ggggcagctg ttcgatgcgg acggtgatgg caaggcggag ccggtgttct
     8881 acaacggcta ccgggcaggc aaggaaatct ccggcccaac agacccggac gacgctggag
     8941 cgatcggcaa tgtgccggcg aaaaccacga ccgccgtgca tgcactggga gtggaaaaca
     9001 tggcggtgca ctgcgtccag ggccgcggcg tcatgatcga tttgcatgcg cacctcggtc
     9061 gggccggcac ggcggtcggt tacgaccagc tcatgcgaat tatggaaagc gacaaagtgg
     9121 tcgtcgaaaa gggcgatatg gtttgtttgc acaccggttt tgcccaaatg attctggaaa
     9181 tgaacaagca gccggatttg cacaagctcg aacattcctg cgccgcactc gatgggcgcg
     9241 accgaaagct gttgcaatgg atcacggatt ccgggcttgt ttcgctgatc gcagacaatt
     9301 acgcggtgga agcacacccg gccaccagtc atcatggaag ctgcgcgagc ctgcccctgc
     9361 acgagcattg cctgttcaag cttggcgtca acctcgggga gatctggcat ctcacgccgc
     9421 tcgccaactg gttgcgcgaa cacaagcgtt cccgctttct gttgacggct ccgccgctgc
     9481 gattacccgg tgcggtcggg tcgccggcaa cgcccgtggc cacggtttaa cctatctgaa
     9541 actttgccat gccaacacga gcgtccgcga tcaattctgt acagaaatca tgtcggatcc
     9601 tgcgttcact gacagagccc agagtgaccc ggctgtctga aatcgcgggg caagcaggcc
     9661 tgaacaaggt cacgactctg cgtattcttg aagtcttgtg ccgtgaagga ttcgtcaagc
     9721 gcgacgataa gaccaagacg tattcgcttg gcaacgaggt gttcattctc gcagccgcgc
     9781 tgcgcgatcg ggatgacttg cgggctcgtg cgcggccttc gctggtgcgc ctggctgcaa
     9841 tgtccgaaga ttccgtgttg ctttctgtac gcagtggcgg caccgagtcg gtctgcgtag
     9901 accgtgaagt gggcagtttt ccaatacgcg ctaattatct ggacatcggc agccggcgcc
     9961 cgctgggcat gggcgctggt gcgatggcgc tactcgcctg gcaaccgcag gctgagatcg
    10021 aggccatctt gccgttggtg gagcagcggc tgccgcagta tccgaagatc agcctggcat
    10081 ggctcaagag cgaaatggag cgctcgcgcg agcgcggtta tacggtgttc ctgaacatgc
    10141 tggtcgagcg catgggagca gtcggtgtgc cgattttcgg gtccgacggg agcgcagtcg
    10201 cggcaatcag cattgccgcg ctctccgacc ggctttcttc gcgcatggac caactagtcg
    10261 ggatgctcaa gcgcgaggca gaagcaattg gtaatccgaa aagaacatag ccgtgtcccg
    10321 tctcgccgga tagcgggcta ccgcccataa tggatgttgg cagcatcacg gccgtgactg
    10381 ggtaactgcg agacgatgac atacgtgttg agaccggttc ttgaacaatt cggcccggaa
    10441 cagattcaag ctcatacgca cccgtcacga ccgtcgcgct tgcggagttc tccttgagct
    10501 tcatcaccgg cgccggtctg acgccttacg gcaagcaacc cgataaaaac acgctggacc
    10561 tcatgagcga ggcggcagca tcggcgctgg cggatgcgca actcgagcgt gcggacatcg
    10621 atgggctggt gtgcggctac tcaacaacaa tgccccacct gatgttgtca acggtgttcg
    10681 ccgagcactt tggcctgaat ccggcttatg cgcatgcgat acagcttggc ggagcaaccg
    10741 gttttgccat ggctatgctg gcgcatcacc tggtcgactc gcgtgtggcg gacaatgttt
    10801 tggtcgtcgc cggcgagaac aggctcaccg ggcaaacaag agattccgcg atacagacgc
    10861 tggcccaggt cggccacccc gtctacgaag tgccgctcgg cgctaccatt cctgcgtact
    10921 acgggctgct agcttcgcgc tacatgacgg agtacggcac aacggaatcg gatttggccg
    10981 agcttgccgt cctcatgcgc aagcatgctt gcatgcatcc tggcgcgcaa ttcagaacgc
    11041 ccattacggt agacgccgta ctggagtcca agccaatcgc ttcacccctc aaactactcg
    11101 actgctgtcc ggtgtcggac ggcggggcgg catttgtcgt ccgccgcgtc cccggtaacg
    11161 gccacgcggt ccgcattcgc ggtaccggcc agtcgcacac ccatcaacac atcagcgccg
    11221 caccgagcct gacgcagttc gctgccggag cttcagtggc caaggcgctt ggcgctgcgg
    11281 gcgcggagct gggtgacgtg aactacgccg cgatctatga cagcttcact atcacgctca
    11341 cgatcctgct tgaagaaata ggacttgcgc cacgcggcgg tgccggccgc caggcgcgcg
    11401 aagggcacta tgaccatgac ggcccgctgc cgctgaacac ccatggagga ctcctttcct
    11461 atggccattg tggcgtcggt ggtgcaatgg cgcacctggt ggaggcgcaa cgccagctga
    11521 cgaaccgtgc ccaaaaccgg caagccggca acgcgacgct ggcgttattg catggagacg
    11581 gcggtgtgct gtcttcgcat gtgtcgctcg tactggagag agtcgcatga atccactcga
    11641 ctggaccaac gatggggatg gcattaccta tcagtgctgc cgccagtgcg attcgagatg
    11701 gtattttcag cgcggttttt gcccgcgctg cggtagttcc aatccaacaa cgctgcaggc
    11761 atcgggaatc ggcatggtac atgcgcgtac cctggtctcg cgtgcaccga ccgaggagtg
    11821 gaaagcgcac tcgccctacc tgattgtcat gatcgatgcc gacgaaggct tccgtttgat
    11881 ggcacatggc agtatggaat tacagattgg aacgcgggta cgagcgcgct tcatcgcgct
    11941 tgccggacgc aaaattccct atttcgagaa gttccagtag tcgacggaac catgcaccat
    12001 cggccgggcc acgaacaaca agcgtaaatt
//
LOCUS       DATMZQ010000282         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4605414, whole genome shotgun sequence.
ACCESSION   DATMZQ010000282 DATMZQ010000000
VERSION     DATMZQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4605414"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VN496_09250"
     CDS             <1..380
                     /locus_tag="VN496_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXF17179.1"
                     /translation="LQEEGRNVAMAGDGINDAPALAQADVGIAMGTGTDVAMESAGVT
                     LVKGDLRGIARAALLSRATMRNIRQNLAFAFGYNAIGIPIAAGVLYPVFGLLLSPVFA
                     GAAMALSSVSVVTNALRLNRIKL"
     gene            complement(476..1108)
                     /gene="tpx"
                     /locus_tag="VN496_09255"
     CDS             complement(476..1108)
                     /gene="tpx"
                     /locus_tag="VN496_09255"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HXF17180.1"
                     /translation="MKKSMLASVLMATLLGIGAGTAFAAGEEVQGIKVNKGNGMAGDG
                     NTVAMKGSPLPLKGQALKVGEPLPSAVVTAANLSPVDIATGNGKVRIISVVPSVDTPT
                     CEAQTHQLSEKDRKLADQVEMVTISMDLPFAQSRFAKEAKIKNVTFYSDYKTGAFGMN
                     NGLLIDPLHLLARAVIVTDKNNIVRYLQVVPEVTELPDMAAAMSAAKALL"
     gene            complement(1223..1858)
                     /locus_tag="VN496_09260"
     CDS             complement(1223..1858)
                     /locus_tag="VN496_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXF17181.1"
                     /translation="MKTVRIVIFAKAPIAGAVKTRLIPALGAQGAARLAHRMLTHTVA
                     EAVAAGAGPVELCVSPSPIASVWQSVALSTNVSWSDQSHGDLGTRMARAVRRVTEAGE
                     SIVLIGTDCPQLDRSLLRAAASSLQHYDATISPTTDGGYVLLGLRRYDPALFRDIVWS
                     TPTVASETLSRLTQLGWTVQHNPIVRDIDQPDDLAYLPPSWLDLQQAEHGS"
     gene            complement(1855..>2499)
                     /locus_tag="VN496_09265"
     CDS             complement(1855..>2499)
                     /locus_tag="VN496_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXF17182.1"
                     /translation="NEAHALASLLEQLGRLAHQGTEVILSDGDSEDGSASMAEQAGFT
                     VVQSTRGRARQMNAAAAGAKGDVLLFLHADTRLPDHAGQYIAQALAAGTHCWGRFDVR
                     ISGRPYLLRVVSRLMNVRSRITGIATGDQAIFVTRSAFDAVGGFPDQPLMEDIELSKR
                     LLAHSKPARIRQPVITSGRRWEQDGVWRTIVLMWRLRWNYWRGVPAQQLAKIYR"
BASE COUNT          481 a          752 c          743 g          523 t
ORIGIN      
        1 ggctgcagga ggagggccgc aacgtggcca tggcgggtga tggaatcaat gatgcgccgg
       61 cactggcgca ggccgatgtc ggtatcgcga tgggaaccgg caccgatgta gccatggaaa
      121 gtgccggggt gacgctggtc aagggcgact tgcgcggcat cgcgcgcgca gcgctgctgt
      181 cgcgcgcgac catgcgcaac atccggcaga atctggcatt cgcgttcggt tacaacgcaa
      241 tcggcattcc gattgccgct ggcgtgctgt atccggtgtt cggcctgttg ctgtcgcctg
      301 ttttcgccgg cgccgcgatg gcgctcagtt cggtctccgt ggttacgaac gccttgcggc
      361 tgaatcgcat caagctgtga acagcgatgg aaatcgtttg acggcggatt caattccaaa
      421 aaaagaagcc ccgcactcgc ggggcttcgc atctactgct gcaaccggtc gaaaattaga
      481 gcaacgcttt tgcggcactc atggctgcgg ccatgtcagg cagttcggta acttcaggca
      541 cgacttgcag gtaacgcacg atattgtttt tatcggtcac gatgaccgcc ctcgcgagca
      601 ggtgcagagg atcgatcagc aggccgttat tcatgccgaa tgcgccagtc ttgtagtcag
      661 aataaaacgt gacattcttg atcttggctt ctttcgcaaa gcgagactgg gcaaagggca
      721 gatccatgct gatggtgacc atctcgacct ggtctgccag cttgcggtcc ttttcactga
      781 gttgatgtgt ttgcgcctcg caggtcggcg tatcgaccga cggtacgacg ctgatgatgc
      841 gcaccttgcc attgccggtt gcaatatcga ccgggctcag attagctgcg gtgaccaccg
      901 ccgacggcag gggttcgcca accttgagcg cctgcccctt cagcggcagg gggctgccct
      961 tcatcgccac ggtattgccg tcgccggcca tgccgttgcc cttgtttacc ttgataccct
     1021 ggacttcttc gccggccgcg aacgcggttc ccgcgccgat gcccagcaaa gtcgccatta
     1081 aaacagacgc caacattgat ttcttcattg aagttcctcc acgaaaatta tctggatgct
     1141 ccctgtactg gcgcagccgg gcgattttct gacggattcg cgcattgcac cagcgaaaat
     1201 tcttggttgc gcctcgtgat tttcatgatc cgtgctcggc ctgctgcaag tcaagccagc
     1261 tcggcggcag atacgcaagg tcatccggct ggtcgatgtc gcgcacaatc ggattgtgtt
     1321 gcaccgtcca gccgagctgc gtgaggcgcg acagcgtttc ggatgcgacc gtcggcgtac
     1381 tccacacgat gtccctgaag agcgcagggt cataacgccg caaacccagc aacacgtatc
     1441 cgccatcggt tgtgggcgag atggttgcgt catagtgctg taacgaacta gcagctgcgc
     1501 gcagcagtga gcgatctaat tgcgggcagt ccgtgccgat gagcacgatc gattcaccgg
     1561 cctcagttac ccgccttacc gcccgcgcca tgcgtgttcc caaatcgccg tgactttgat
     1621 ccgaccacga tacatttgtc gatagggcga cggactgcca caccgatgca atcggtgaag
     1681 ggctcacaca cagttccacc ggacccgcgc cggcagccac ggcttctgca accgtgtgcg
     1741 tcagcatccg atgagcaagc ctggccgcgc cttgtgcacc gagcgcagga atcaatcgcg
     1801 ttttgacggc acctgcgatt ggcgcctttg cgaagatcac aatacggaca gttttcaccg
     1861 ataaatcttg gcgagttgct gggcgggaac gccacgccag taattccaac gcaaccgcca
     1921 catcagcaca atggtgcgcc atacaccgtc ttgctcccag cgtcgtcccg atgttatgac
     1981 gggctgtcgg atacgcgcgg gcttcgagtg tgccagcagc cgcttggaaa gttcgatgtc
     2041 ttccatgagc ggttggtcgg gaaaaccgcc aactgcatcg aaggcactgc gcgtcacaaa
     2101 tatggcttga tctccggtcg caatgccggt tatgcgcgag cgaacattca tcagacggct
     2161 caccacgcgt agcagataag ggcggccgct gatgcgcaca tcgaagcggc cccagcaatg
     2221 tgtgccggca gccagcgcct gcgctatgta ttggccagcg tggtcgggca gccgggtgtc
     2281 ggcgtgcagg aacaacagta catccccctt ggctccggcc gctgccgcgt tcatctggcg
     2341 ggcgcgtccg cgggtcgact gcacgacggt gaagccggcc tgctcggcca tggatgccga
     2401 gccatcttca ctatccccat ccgacaagat gacttcggtg ccttgatgcg caaggcgccc
     2461 cagttgttcg agcaagcttg ccagcgcgtg tgcttcatt
//
LOCUS       DATMZQ010000283         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_579716, whole genome shotgun sequence.
ACCESSION   DATMZQ010000283 DATMZQ010000000
VERSION     DATMZQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579716"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VN496_09270"
     CDS             <1..964
                     /locus_tag="VN496_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXF17183.1"
                     /translation="FRCRREDALGSSLDRFMPTRFRGAHRGHIERFGKTGVTSRRMGD
                     VTTLWAVRLDSGEEFPIEASISQATENGHHYYTVILRDITARKHYEDELKQQQSELRK
                     LSAQVLEAREEEKTRIARELHDELGQMLTALKMDLGWVRGRIGGSDGELAAKTNKMWE
                     TLDQTVTSVRRIAADLRPLMLDDLGLADAAAWLVEDFSKRSDLRCEFKIINDSDTSDF
                     ESNVANTVYRALQESLTNVGRHANAKNAWVLLRIDERAIRLEIEDDGRGISAEDMTKS
                     HSLGLKGMRERVFHIGGVLEVTSAPRGGTRVSVFVPVRPPGAPS"
     gene            961..1614
                     /locus_tag="VN496_09275"
     CDS             961..1614
                     /locus_tag="VN496_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF17184.1"
                     /translation="MIRLLIADDHKLVRDGLKQILAAAAGIDVAAEAANGDEALALVK
                     AGEFDVALIDMSMPGLAGIALIKRLKLERPKLRILVLSMHGESQYAARALKAGASGYL
                     TKDSASEQLVIAIRKIAAGGVFITDAAAASLMSNSSAHDSPSHEHLSDREFEIFRLLA
                     AGRGPTEISEQLRLSVKTISTHKTRILEKLNLANTGELVRYALEHGLLDGTSQSGRG"
     gene            1728..2159
                     /locus_tag="VN496_09280"
     CDS             1728..2159
                     /locus_tag="VN496_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF17185.1"
                     /translation="MMNVSSTTSGTSNPLPVFIVEASQAIRERLVDMITAIDGTCCAG
                     AATIAAIPDILQAHPDTVILDIRLPEGNGFDVLRALRHHAPDIQIYAVSNHSTPAHRQ
                     LAYRLGASAFFDKTIEIEAMRELIAQRAARRLRITTTSITQ"
     gene            2339..3010
                     /locus_tag="VN496_09285"
     CDS             2339..3010
                     /locus_tag="VN496_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXF17186.1"
                     /translation="MRELCLTSGLCPSDLERAETMVHARRRIKRGEVLFNGGETFNSV
                     YAIRAGFFKSSVLDNEGREQVTGFYMGGELLGMEGIGSGQYNATATALEDSEICAMPF
                     ALIEEMSHELRPLQRHLHIVLAREIVRDHGVMMLLGSMRAEERLAVFLLNLSQRFTAR
                     GYSPSEFYLRMTREEIGSYLGLKLETVSRAFSKFQESGLVTVQQKHIRIINIPGLRKM
                     AGQNG"
BASE COUNT          664 a          879 c          883 g          585 t
ORIGIN      
        1 atttcgttgc cgtcgcgaag atgcgctggg cagttcactg gaccggttca tgccaacccg
       61 cttccggggt gcgcatcgcg ggcacatcga gcggttcgga aaaaccggtg tgaccagccg
      121 gcgcatgggg gatgtgacca cgctgtgggc cgtccgcctg gatagcggcg aagaatttcc
      181 gattgaagcc tcgatttcgc aggccacgga gaacggtcac cattactaca cggtcattct
      241 gcgcgacatc accgcacgca agcactacga ggacgaactg aagcagcagc agagcgaatt
      301 gcgcaagctt tcagcgcagg tgctcgaagc tcgcgaggaa gaaaagacac gtatcgcccg
      361 cgaactgcat gacgagctcg gccagatgct gaccgcactg aaaatggact tgggctgggt
      421 gcgaggccgc attggcggca gtgatggaga attggccgct aaaaccaaca agatgtggga
      481 gacgctcgac caaacggtaa cctcggtacg gcgcattgcc gccgatttgc gaccattgat
      541 gcttgatgac ctcggcttgg cggatgccgc tgcctggctg gtggaggatt tcagcaaacg
      601 ctcggacctg cgctgcgaat tcaagatcat caacgactcg gacacgagcg acttcgaaag
      661 caacgtggcc aatacagtat atcgcgcgct gcaggaatcg ctgaccaacg tcgggcgaca
      721 tgccaatgca aagaacgcct gggtgctgtt gcgcatcgat gagcgagcca tccggcttga
      781 aatcgaagat gatggccgcg gcatatccgc cgaagacatg acgaagtcgc actcacttgg
      841 attgaaaggc atgcgcgaac gcgtttttca tattggcggc gtgcttgaag tgaccagtgc
      901 gccgcgcggc ggcacgcgcg tcagcgtctt tgtgcccgtg cgcccacccg gagcgccttc
      961 gtgatacgac tgctcatagc ggacgaccac aaactggtgc gcgacggact gaaacagatt
     1021 ctcgccgccg ctgcgggcat cgacgttgcc gcggaggcgg caaacgggga cgaagctttg
     1081 gcgctggtca aggccggtga attcgatgtc gcgctgatcg acatgtccat gccgggcctt
     1141 gccggcattg cgcttatcaa gcgcttgaag ctggagcgac caaagttgcg catactggta
     1201 ttgtcgatgc acggggagtc gcaatacgcc gctcgcgcat tgaaggcggg cgcgtcggga
     1261 tacctcacga aggacagcgc atccgagcag ctcgtcatag ccatccgcaa aatcgcggcc
     1321 ggcggcgtat tcattaccga tgcagcggca gccagcctga tgagcaactc ttcagcgcac
     1381 gactcgccat cgcatgaaca cctgtccgac cgggagttcg aaatcttccg gctcctcgcg
     1441 gcgggacggg gtccgaccga aatctcggag cagttgcgtc tctcggtcaa gaccatcagt
     1501 acgcataaaa cgcggatcct ggaaaagctg aatcttgcca ataccgggga attggttcga
     1561 tacgcgctgg agcatggact gctggacggc acttcccaat ccggtcgcgg gtaggaattt
     1621 tcctaattct attttccttt cttcccaagc cgtttcgcgc gcgttcctag cgtcatatta
     1681 agctggcgcc gataggcgcc ggatgccgtc gcatcgatac tggcggcatg atgaatgtat
     1741 cctcaaccac ctccggaaca agcaaccctc tgccagtctt catcgtcgaa gcttcgcaag
     1801 ccatacgcga gcgcctggtg gacatgatta ccgccattga tggaacctgc tgcgcgggtg
     1861 cggcgactat tgccgccatt cccgacatac tgcaagcgca tcccgatacc gtcatactgg
     1921 atatccggct gccagaaggc aacggcttcg acgtcctgcg tgccctccgg catcatgcgc
     1981 ctgacattca gatttacgct gtcagcaacc attccacacc cgcccatcgc caactggcct
     2041 atcgtttggg cgccagtgct ttttttgaca aaaccatcga aatagaagcg atgcgagagc
     2101 tcatcgcgca gcgtgccgca agaaggctgc gcattaccac tacctcaata acacaatgac
     2161 tcaataactc aataactcaa tcgccaaagg ggaacccatg cgaacagccg cgatgaagtt
     2221 agcatccgtc actttacgcc gagcgacggt cacgaaaatg gacgtttgcg tgattccgat
     2281 cacgcgcgat gccaaaggcg acgaggcggc gccgcgcatt acctgctcga cctgcaacat
     2341 gcgcgaattg tgcctgacga gtggactgtg tccctccgac ctggagcgcg ccgagacgat
     2401 ggttcacgcc cgccggcgca tcaagcgcgg cgaagtcctg ttcaatgggg gcgaaacctt
     2461 caattccgta tatgccattc gtgcaggatt cttcaagtcg agcgtgctcg ataacgaagg
     2521 gcgggagcaa gtcactggtt tttacatggg cggggagctg ctgggcatgg aaggaatcgg
     2581 cagcggacag tacaacgcga ccgcgaccgc gctcgaagac agcgaaatct gcgccatgcc
     2641 ctttgcgctg attgaggaga tgtcgcacga gttgcgtcct cttcaacggc atttgcatat
     2701 cgtgctggcc cgggaaatcg tgcgtgacca cggcgtcatg atgctgctcg gctccatgcg
     2761 cgccgaggag cgcctcgccg tgttcctgct caatctctcg cagcgcttca ccgcgcgcgg
     2821 ctattctccg tcggaatttt atctgcgcat gacacgcgag gagatcggca gctatcttgg
     2881 gctgaaactg gaaaccgtca gcagagcctt ctccaaattt caggaatccg ggctggtgac
     2941 cgtccagcaa aagcatattc ggattattaa cattccaggc ttgcgcaaga tggcgggaca
     3001 gaacggctga a
//
LOCUS       DATMZQ010000284         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2189883, whole genome shotgun sequence.
ACCESSION   DATMZQ010000284 DATMZQ010000000
VERSION     DATMZQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189883"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VN496_09290"
     CDS             complement(<1..641)
                     /locus_tag="VN496_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HXF17187.1"
                     /translation="MRFDKFTTRFQQALADAQSTAVGQDNPYIEPQHLLLALLEQDDG
                     GTSALLSRAGVNVAPLKEALRASISRLPKVEGQGGEINVSRELSSLLNLTDKEAQKRG
                     DQFIASELFLLAAAQDKGEVGKLLKTHGVNKKALEQAIEAVRGGQGVQSQEAEGQREA
                     LEKYTLDLTERARRGKLDPVIGRDDEIRRVIQILQRRTKNNPVLIGEPGVGKTA"
     gene            972..1640
                     /locus_tag="VN496_09295"
     CDS             972..1640
                     /locus_tag="VN496_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HXF17188.1"
                     /translation="MSAVIRALVFDAYGTLFDVHSISNACESLFPGKGSELSRLWRTK
                     QLEYTWLRSLMGRYVEFETITRDALIHSCGVLGLALDQSLSIVLMQSYRKLSLFPEVM
                     DALGALNKCKLAILSNGSPSMLNTLVEHAGMDRVFDTVISVDELGIFKPHPSVYALAV
                     QRLNVESGEIGFVSSNFWDISGAASFGFRTFWINRNNVPPDKLGFQPIAVLRRLDELP
                     ACLT"
     gene            1694..2539
                     /gene="nadC"
                     /locus_tag="VN496_09300"
     CDS             1694..2539
                     /gene="nadC"
                     /locus_tag="VN496_09300"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXF17189.1"
                     /translation="MERQLELNLASAIQQDVRKALAEDIGTGDVTARLVPASETARAQ
                     VKTRVPAVLSGMAWFEECFRQIDDEIEIHWRANDGDRLSREQLVCELRGPARNLLTGE
                     RTSLNFLQTLSGVATRTRAFVDAIAGTKAVILDTRKTIPGLRMALKYAVHCGGGTNHR
                     MGLYDGILIKENHIASAGGIAAVLLQAGKIAQHIPTEIEVENIDQLNQAITAGAKLIL
                     LDNFDVDRLREAVKATAGRALLEASGGINLETVRAIAETGVDRISVGSLTKDIQAIDL
                     SMRFF"
     gene            complement(2649..3773)
                     /gene="thrC"
                     /locus_tag="VN496_09305"
     CDS             complement(2649..3773)
                     /gene="thrC"
                     /locus_tag="VN496_09305"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXF17190.1"
                     /translation="MLNHYNGLIDRYRERLPVSATTPIVSLNEGNTPLIELLHLPKIL
                     KKNVRILAKFEGLNPTGSFKDRGMTMAVTKAIEAGARAIICASTGNTSAAAAAYAARA
                     GISCFVLIPEGQIALGKLSQAMMHGSVVIQIRGNFDDGMKLVQEVAHSMPVNIVNSIN
                     PYRLHGQKTISFEICEALGDCPDYHVLPVGNAGNVSAHWMGYSEAAGVKTESSPRLDT
                     GPPYKMGGLTRKRPIMVGYQASGAAPFLRGAMVDRPETVATAIRIGHPVSWKLAWAAK
                     NESGGWFNECSDQEILKAQKILAQSEGIFCEPASAISIAGLIKDLEQSVIGEGSTIVC
                     TLTGHGLKDPDTAIGQISKPFLVDATRAAIEKVIGANLPG"
     gene            complement(3777..>4158)
                     /locus_tag="VN496_09310"
     CDS             complement(3777..>4158)
                     /locus_tag="VN496_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXF17191.1"
                     /translation="DLVDVTRMHTADPEQRVPHLAFQPDRMSDLNILSMEQVETSYYL
                     RLRVIDRPGVLADITRILADQSISIDAMVQKEPGEGEEQVDIIMLTHLTVEKNINRAI
                     ERIESLPVVSGKVTRIRLEELGRN"
BASE COUNT          914 a         1110 c         1132 g         1002 t
ORIGIN      
        1 gccgtcttgc ccacgcccgg ttcgccgatc aggacaggat tgtttttggt ccgacgctga
       61 aggatctgaa tgacccgccg gatctcatcg tcgcgaccaa taaccggatc gagtttgccg
      121 cgccgggcgc gttctgtgag atcgagggtg tatttctcca gcgcttcccg ctgtccttcg
      181 gcttcctggc tctgcacgcc ttgccccccg cggacagctt caatggcctg ctcaagcgct
      241 ttcttgttga ccccgtgagt cttcaaaagc ttgccgactt cccccttatc ctgggctgcc
      301 gcaagcagaa agagttcgga cgctatgaac tggtccccgc gtttctgggc ttccttatcg
      361 gtcaaattaa ggaggctgga tagctctcga gagacgttga tttcgccgcc ttgaccctcc
      421 accttgggca gccgacttat agaggcacgc aacgcctcct tcaacggagc aacattgaca
      481 ccagcgcggg acagcaaggc tgacgtaccg ccgtcatcct gctccagaag tgccagcagc
      541 aggtgttgtg gctcgatata gggattatcc tggccgactg ccgtactttg ggcatcagcc
      601 aaggcttgct ggaatcgggt ggtgaattta tcaaaacgca tctctaaatc ctcactgggg
      661 ttcacatgta cgttgagtgc tgacgtagat tgggtaactt tcggaaattt caacatcccg
      721 caggaccgac agtctggtgc gcaacgcggc acgtcgacgc ttgaatggac cctaccaaag
      781 cggcgggtgc cggtggtggc taagttaatt acgtaaaggc gcttgcagcg ctgcccaatg
      841 aaaggttccg aatcaacgcc tcctcagcca aggctaaggg gtaatatcga ggctaataag
      901 ggatcgggga tttccatgcc ggacaagagc gaagaggcaa ttcagaccaa tttgagcacg
      961 atggcattgc gatgagtgcg gtgatacgag cgctggtgtt cgatgcttat ggaacgttgt
     1021 tcgacgtcca ttccattagc aatgcctgcg aatcattgtt tcctggaaaa ggctcggagt
     1081 tgtcccggct atggcgcact aaacaattgg aatacacctg gttacggagc ctgatgggcc
     1141 gctacgtcga gttcgagacg attacgaggg atgccctcat tcacagctgt ggagtactcg
     1201 gactcgcttt ggatcagtca ctgtccatag tcttgatgca aagctatcga aagctttctc
     1261 tatttccgga agtcatggat gcactgggcg ctttgaacaa atgcaaactt gccattctgt
     1321 ccaacggttc gccttctatg ctaaacaccc tggtagaaca tgccgggatg gatcgggtat
     1381 ttgatacagt catcagcgtg gacgaactcg gtatcttcaa gccacatccc agcgtatacg
     1441 cgcttgcggt ccaacgcttg aacgttgaat caggggagat cgggtttgtc tcctccaatt
     1501 tttgggacat tagtggcgcc gcttcgttcg ggtttcgcac cttttggatc aaccgcaaca
     1561 atgttccgcc ggataaactc ggattccagc cgatcgctgt tctgcgacgg ttggacgaat
     1621 tgccagcctg cttaacctga ttgataccgt ttaatgccga cgcgcttgct tgccctatag
     1681 ctgttatgga gccatggaaa ggcaactgga attgaatttg gcatccgcaa tccagcagga
     1741 cgttcggaaa gcgctagctg aagacattgg aaccggcgat gtcactgcga ggctggtgcc
     1801 cgcatccgag acagcacgtg cccaagtcaa gacacgagtg cctgcggttt tgagtgggat
     1861 ggcatggttc gaggaatgtt tccgccagat cgacgatgaa atcgagattc actggcgtgc
     1921 aaatgatggc gaccgtctct cacgtgagca actggtgtgc gaactacgag ggcctgcgcg
     1981 caatttgctg actggtgaaa gaaccagcct gaatttcctt cagacattgt ccggagtcgc
     2041 gaccaggacg cgggccttcg tcgatgccat agcgggcaca aaggcagtca tcctcgacac
     2101 gcgaaagaca attccgggat tgcgaatggc actcaaatac gctgtgcact gcggtggtgg
     2161 caccaatcat cgtatggggc tttatgacgg aatactcatc aaggaaaacc atatcgcgag
     2221 cgcgggaggc attgccgcgg tgctgctaca ggccggcaaa atcgcacagc acataccgac
     2281 tgaaatagag gtcgaaaata tcgatcaatt gaaccaagcc atcacggctg gggcgaaatt
     2341 gattctgctg gataactttg atgttgatcg ccttcgcgag gcggttaagg caacggcagg
     2401 gcgtgcactt ctggaagctt ccggtggcat caatctggaa acggtcaggg ccatcgcaga
     2461 gaccggtgtc gatcgcatat ccgtcggatc gctgacgaag gatattcagg ccatcgattt
     2521 gtcgatgcgt tttttttaga cgaatacctt ccgcacttcc cacgaagttg accgttctcc
     2581 accacgccgt cataaggcat cgaacactgc aaatgagcag aactttggct tcgggcgtcc
     2641 attgacgcct atccaggcaa gttcgcgcca atgaccttct cgatggcagc gcgcgttgcg
     2701 tctaccagga acggtttgct gatttgtccg atggcagtgt ctggatcttt caatccatgc
     2761 ccggtcaggg tgcagactat tgttgagcct tcccctatta cgctttgttc aagatcttta
     2821 atcaggccgg cgattgagat ggctgaagcg ggttcgcaaa agataccctc gctttgtgcc
     2881 agtatttttt gtgctttaag gatttcctgg tcactgcact cattgaacca gcctccagat
     2941 tcgtttttcg cggcccatgc gagcttccac gagactggat gaccgatgcg gatcgccgtt
     3001 gccaccgttt cggggcgatc gaccattgca ccacgcagaa agggagcggc gccggacgcc
     3061 tgataaccca ccatgatggg acgtttcctg gtgagtcctc ccattttgta tggcggtccc
     3121 gtgtccaggc gcggcgaact ttcggttttc acacccgcgg cttcgctgta acccatccaa
     3181 tgcgccgaca cgtttccagc attaccgacc ggaaggacat ggtagtcagg acaatcgccc
     3241 agcgcttcac agatttcgaa tgaaatagtc ttctgtccat gcaaccggta cggattgatg
     3301 gaattcacga tatttaccgg catgctgtga gcgacttcct gtaccagttt catgccatcg
     3361 tcaaaatttc cgcgaatctg tatgaccaca gaaccgtgca tcattgcctg ggacaacttt
     3421 cccagtgcga tctgaccttc cggtattagc acgaagcagc tgatgccggc gcgcgcggca
     3481 taggccgccg ctgcggctga agtgttgcct gtcgatgcac agatgattgc gcgggcgccg
     3541 gcctcgatgg ctttcgtgac agccatcgtc atgccgcgat ccttgaagga accggttggg
     3601 ttaagacctt caaacttggc cagaatacga acgttcttct tgagtatctt cggcagatga
     3661 agcagttcaa tgagcggcgt attgccttca ttcaaactga caatcggagt ggtcgcgctg
     3721 acgggaagac gttcccgata gcgatcgata aggccattgt aatgattcag catgttttaa
     3781 ttgcgtccga gttcttcgag ccggattcgc gtcacttttc cggacacaac tggcagcgat
     3841 tcgatgcgct cgatagcacg attgatgttt ttttcaacgg tcaggtgagt gagcatgatg
     3901 atatctactt gctcctctcc ttcaccgggt tccttctgca ccatagcatc tatcgagatt
     3961 gattgatccg cgagaattcg cgttatgtcg gccaacaccc cgggacggtc tataacgcgc
     4021 aatcgcaggt aatatgatgt ctccacttgc tccatcgaca aaatgttcag atcggacatg
     4081 cggtcaggct gaaatgcaag atgtggcacg cgctgttcag gatccgccgt gtgcattcgc
     4141 gtaacgtcca ccaggtca
//
LOCUS       DATMZQ010000285         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_809527, whole genome shotgun sequence.
ACCESSION   DATMZQ010000285 DATMZQ010000000
VERSION     DATMZQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809527"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..620)
                     /gene="ribH"
                     /locus_tag="VN496_09315"
     CDS             complement(141..620)
                     /gene="ribH"
                     /locus_tag="VN496_09315"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXF17192.1"
                     /translation="MANDQEIFEYRSDLNGAGLRIGIVVGRFNQDVGDGLLSACVAEL
                     GKLGVTNGDIQVVTVPGALEIPLALQKLASTQRFDGLVALGAVIRGETYHFEVVANQS
                     ADGVMTVQLDTGLPIANGILTTDDDDQAEARMHQKGMDCARVVVEMANLMQEINEEQ"
     gene            complement(650..1789)
                     /gene="ribBA"
                     /locus_tag="VN496_09320"
     CDS             complement(650..1789)
                     /gene="ribBA"
                     /locus_tag="VN496_09320"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HXF17193.1"
                     /translation="MDSRIDTMTTMSSTAEIIADLRAGKMVILVDEEDRENEGDLVMA
                     AEFVSPEAVNFMAKHGRGLICLTLTEERCRQLDLPLMVGDNRSRMGTNFTVSIEAASG
                     VTTGISAADRARTVRAAVKKDAKPEDIVQPGHIFPLMAQKGGVLVRAGHTEAGCDLAQ
                     LAGLVPAAVICEVLKDDGDMARLPELFAFASEHRLRIGTIAELIHYRSRTESLVERVA
                     ERPLQTIYGQFKLVAYRDKSSRDTHIALVKGKVAAGQEILVRVHEPLSVADFLDAASH
                     SHSWNVNDALRAIAAAPSGVMVLLHRTESGAELLERLAMNEQAQSRMDLRTYGIGAQI
                     LRDLGVTRMKVMGAPRKMPSMTGFDLEVTGYVQPQSTAKSPGGTS"
     gene            complement(1982..>2730)
                     /locus_tag="VN496_09325"
     CDS             complement(1982..>2730)
                     /locus_tag="VN496_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXF17194.1"
                     /translation="IRSSPNLSFLNSYPLRDRLQNFTGARVFVGHDVQAALYGELVRG
                     AAHKARHVIGVWIGTGVGGALVLDRKLFLGISGRAGDLGNYLVHAVDVSQELPRKEVL
                     DNVASRTAIAGDAAALAASHRAPRLQRTAGTEVRNMGANVLADSISQGDKAVEKLVRS
                     RASVIGVALSNLVDFLNPDMIVLGGGLVEAMPRLLQRQIQKAINAHSAPNSAKAVKVR
                     VAKLMPHAGTIGAACLARDMFSDDPPIDLA"
BASE COUNT          530 a          867 c          742 g          591 t
ORIGIN      
        1 gcaccagcgc ctcggccagt gcctgatccg ctttcgggaa gtctttttgt tctttgagct
       61 gcgcaagcag gctctccgca gccgattcgt tcaacagcca ttggtagatt gcctgaagcg
      121 caaactccct ggcgcgccgc ctactgttct tcatttatct cctgcatgag attcgccatc
      181 tcgaccacga cgcgcgcaca gtccatgccc ttttgatgca tgcgcgcctc ggcctgatcg
      241 tcgtcgtccg tagtcagtat gccgttcgcg atcggcagtc cggtatcaag ctgcaccgtc
      301 atcacaccgt ctgcagattg attcgctact acctcaaaat gatatgtttc cccgcgaatg
      361 acagcgccga gcgcgaccaa tccatcgaaa cgttgcgtgc tggccaattt ttgcaaggca
      421 agcggtatct caagcgctcc cggcaccgtt acaacctgga tgtcgccgtt cgtgacacca
      481 agcttgccga gttccgcaac gcaagccgat aacaagccgt cgccaacgtc ctgattgaag
      541 cggccaacca cgatcccgat acgcagtccg gcgccgttca agtcggatcg gtattcgaaa
      601 atttcctggt catttgccat gcgcgtacct gatttttccg aaagcgatgt taactcgtgc
      661 cgcctggtga ttttgccgtt gattgcggct gcacatatcc ggttacctcg agatcgaaac
      721 ccgtcatgct cggcattttt ctgggcgccc ccattacttt catcctggtc acgccaagat
      781 cgcgcaggat ctgcgcgccg ataccatagg tgcgcaggtc cattcttgac tgcgcttgct
      841 cgttcatcgc caggcgctcc agcaattccg cgcccgactc cgtccggtgc agcaatacca
      901 tcacaccgct cggcgcggcg gcaattgcgc gcagcgcatc gttcacgttc cacgaatggc
      961 tgtggctggc cgcatccaga aaatcggcaa ccgatagcgg ttcatgcacc ctcaccagaa
     1021 tttcctgacc cgcggcaact tttcctttga ccagcgcaat atgcgtgtca cgcgaactct
     1081 tgtcgcgata tgccaccagc ttgaactgac cgtaaatcgt ctgcagcggc cgctcggcta
     1141 cgcgctccac cagcgactcg gtacgactgc ggtaatggat caattcggcg atcgtgccga
     1201 tcctgagccg gtgttccgag gcgaaagcga acaattccgg caagcgtgcc atgtcgccat
     1261 cgtccttgag tacttcacag attaccgcgg ccggaaccag tccggcgagt tgcgccaggt
     1321 cacaacccgc ctccgtatgt ccggcacgta ccagcacgcc gcctttctgg gccataagcg
     1381 gaaatatatg tccgggctga acgatgtctt ccggctttgc atccttcttc acagccgcgc
     1441 gcacggttcg cgcgcgatcg gctgccgaaa tgcccgtggt tactccgctc gccgcctcaa
     1501 ttgacacggt aaagttggtc cccatccggg accggttgtc gccaaccatc aaaggcaaat
     1561 ccagttgacg acagcgctct tcggtcaatg tcaggcaaat cagcccgcgc ccatgcttgg
     1621 ccatgaagtt aaccgcctcc gggcttacaa attcggccgc catgacgaga tcgccttcgt
     1681 tttcccggtc ttcctcgtcc accaggatga ccatctttcc ggcgcgcagg tcggcgatga
     1741 tttctgctgt ggaactcatc gtggtcatgg tgtcgattcg gctatccatg tgctgctgat
     1801 acctcagttt gaatttgcgc gcaggcacaa ctagaaatct ggatggagcg acgcggtggc
     1861 aagggtgcat tgtgccagat cgacgctacg cgggatcgaa atgcatgaac gggtggtaag
     1921 gatggtgttg ccgacgttca ggctcaatag ttcctggctt tgccgccgcg tctgcgtgaa
     1981 gctacgcgag gtcgatcggc gggtcgtccg agaacatgtc tcttgcgagg cacgcggcgc
     2041 caatggtgcc ggcgtgcggc atcagcttgg ctacccgtac cttcaccgcc tttgccgaat
     2101 tcggcgcgga atgcgcgttg atcgctttct gtatctgacg ttgcagaagc cggggcatcg
     2161 cctccaccaa gccgccgccg agcacgatca tatcgggatt gaggaaatcg accagattgg
     2221 acaaggctac accgatgacg ctggcgcgac tgcgtacgag cttctcgacc gccttgtcgc
     2281 cctggctgat cgaatcggcc agaacattgg cgcccatgtt gcgtacttcc gtgccggccg
     2341 tgcgctgcag ccgtggggcg cgatgacttg ccgcaagcgc agccgcatcc cctgcaatag
     2401 ccgtacgact cgcgacatta tccagaacct ccttgcgcgg cagctcttgt gaaacgtcca
     2461 ctgcgtgcac caggtaattg cccagatcgc cggcgcgacc gctgatgccg agaaacagct
     2521 tgcggtcgag caccaatgct ccgccgacgc cggtgccaat ccacacgccg ataacgtgtc
     2581 gagccttgtg cgcggccccg cgtacgagtt ctccatagag cgccgcctgc acgtcatgac
     2641 ccacgaaaac gcgggccccg gtaaaattct ggagcctgtc gcgcagtgga tagctgttca
     2701 aaaaactaag atttggcgac gaacggatga
//
LOCUS       DATMZQ010000286         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1962911, whole genome shotgun sequence.
ACCESSION   DATMZQ010000286 DATMZQ010000000
VERSION     DATMZQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962911"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VN496_09330"
     CDS             complement(<1..939)
                     /locus_tag="VN496_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17195.1"
                     /translation="MKLATRALIAALTILVFCAAMSAQTLRLPQDPRNQAPTVGTGGP
                     VGGPTGLFTIYDGDTIRKGEFTFSVAYSNYDRDPGNVDIVDTPLSFNVGLNDHVELFF
                     KTNGYRGIKVNNPQNLSSFYLPNSQLRFGASLLCSGAAIIQAPERVSGSTSLTGFSPL
                     FRPAGPPCNVGGQPFLQFPFIGGPGPNFGLTGNAIAPPFVSRIGTPTGGNGSFGAASN
                     FPGIGSSVGSILPGIVLATVNLPCTALTGNCNPPTAANPQSIVQVPATFTISPAYLPD
                     APFVNRLYGESSFTDMVVGAKIRLASPNDALGWGFIP"
     gene            complement(1263..>2180)
                     /locus_tag="VN496_09335"
     CDS             complement(1263..>2180)
                     /locus_tag="VN496_09335"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXF17196.1"
                     /translation="DPQQMSIGELERMTRRYASELLDFIGPEKDVPAPDMNTNEQTMA
                     WIMDTYSMHARHTVTAVVTGKPIDLGGSSGRREATGRGILFVINEAVKRFGRTPETTR
                     VVVQGSGNVGGIGAQLLHESGYKVVAISDVHGGIYNPNGIDIPAAMKHLQTTRSFEDY
                     PGVERVTNSELLELECDVLVPAATENQITSQNADRIKCKVLAEGANGPTTAAADQVLH
                     EKGIFVIPDILANAGGVTVSYFEWVQDRMGYFWREDVVNQRLQDTMVASFNDLCRYAD
                     SHSVDTRTAAYMLAIDRVAYDTRMRGIYA"
BASE COUNT          497 a          623 c          591 g          469 t
ORIGIN      
        1 cggaatgaat ccccagccca gcgcgtcatt tggactagcc agacggatct tcgcgccgac
       61 caccatgtcg gtgaagcttg attcaccgta gagccggttc acgaatggcg cgtcaggcaa
      121 ataggctggc gatatcgtaa aagtggccgg cacttgcaca atggactgcg gatttgcagc
      181 tgtcggcgga ttgcagttgc cagtcagggc ggtacaagga aggttcacag tggctagcac
      241 gatgcccggc agaatgctgc cgaccgacga gccgatgccc gggaaattcg aggccgcgcc
      301 aaagctgcca ttgccgccag tcggcgtgcc aatgcggctc acaaatggcg gcgcaatggc
      361 gttaccagtc aaaccaaagt ttggaccagg gccgccgatg aatggaaact gcagaaacgg
      421 ctggccacca acgttgcacg gtggtcctgc gggacgaaac agcggactaa agcccgtcaa
      481 cgacgtcgaa ccgctcacgc gctcgggcgc ctgaatgatg gcggccccac tgcacagcag
      541 cgaggcaccg aagcggagct gcgagttcgg cagatagaag ctcgacagat tctgtggatt
      601 gtttaccttg atgccacgat agccgttcgt cttgaaaaat aattccacgt gatcgttcaa
      661 gcccacgtta aagctcaacg gcgtatcgac gatgtctacg ttacccggat cgcgatcata
      721 attgctgtag gcgacgctga acgtaaactc gcctttccgg attgtatcgc cgtcatagat
      781 agtgaacaga ccagtcggtc cacccaccgg gccgccggtc ccaacggtcg gcgcctggtt
      841 acgaggatcc tggggcaatc tcaaagtctg ggccgacatt gcggcgcaaa ataccaaaat
      901 agtgagcgca gcaataagcg ctctcgttgc gagtttcatc gcaatcctcc tggaaaacga
      961 atatagaaat ttgttttgaa tgccaatgtc gagagttgca tgttcaacta ttcgcaccga
     1021 ttagcagcta gcgcgcgagc agtggcaccc aaaaactgct gcgctctcct aatgctgaaa
     1081 ctctgtctaa ggtggccatc aacgagatta gttgacgcct acgcttgcac cgaaaaatca
     1141 tggcgcaaaa gaaaaatctt cacactagtc cgacgtagag atcacgccag accatacccc
     1201 acctgtttag ggaaatcaag actttttccc caatttgggc ctatttgttt atgtcttttc
     1261 cgtcacgcgt atatgccgcg catgcgcgtg tcataggcca cccggtctat cgccaacatg
     1321 taggctgcgg tgcgcgtgtc gactgagtgg gaatcggcat atcggcagag atcgttgaag
     1381 ctggcaacca tggtgtcctg aagccgctgg ttgacgacgt cttcccgcca aaaataaccc
     1441 atcctgtcct gcacccattc gaagtatgaa actgtcacgc caccagcatt ggcgaggata
     1501 tccgggatca cgaaaattcc cttctcatgc aacacctgat ccgccgcggc tgtggttggg
     1561 ccattggccc cttccgccag cactttgcac ttgatgcgat cggcattctg cgaggtaatc
     1621 tgattttcgg tcgccgccgg cacgaggaca tcgcattcca actccagcag ttcgctgttg
     1681 gttactcgtt caacccccgg ataatcttcg aaggaccggg tagtctgcag atgcttcatg
     1741 gctgcgggaa tatcgatgcc gtttgggttg tagatgccgc cgtgcacatc ggaaatggct
     1801 acgactttat aaccggattc atgcagcaat tgcgccccga ttccgccgac attaccggag
     1861 ccctgaacta ccacacgcgt ggtctcaggc gttctcccga accgtttgac cgcttcgttg
     1921 attacaaaaa ggattccccg gccggttgcc tcgcgtcgtc cagacgagcc gccgaggtcg
     1981 atcggcttac cggttaccac cgcagtgacc gtatgacggg cgtgcatgga ataggtatcc
     2041 atgatccaag ccatggtctg ttcgttggtg ttcatgtcag gagccggaac gtccttctcc
     2101 ggaccaatga agtccagcag ttccgacgcg tagcgccgcg tcatgcgctc cagctcacca
     2161 atcgacattt gttgtgggtc
//
LOCUS       DATMZQ010000287         5020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1501197, whole genome shotgun sequence.
ACCESSION   DATMZQ010000287 DATMZQ010000000
VERSION     DATMZQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501197"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..2220)
                     /gene="uvrD"
                     /locus_tag="VN496_09340"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(34..2220)
                     /gene="uvrD"
                     /locus_tag="VN496_09340"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HXF17197.1"
                     /translation="MSFLEGLNPQQLEAVTLPHQSALILAGAGSGKTRVLTTRIAWLI
                     QTGQLSPQGVLAVTFTNKAAREMLLRISAMLPINTRGMWVGTFHGLCNRILRAHHRDA
                     GLPQTFQILDSADQLAMIKRVMKSMNIDDEKYPPKQAQYTINAAKDQGLRAAKVEAFD
                     DFSRKLATVYDAYDTQCNREGAVDFAELLLRCFELLAKNEVLREHYRARFRHILVDEF
                     QDTNRLQYLWLKLLAGPSNVIFAVGDDDQSIYAFRGARAGNMLDFEKDFHVEKVIKLE
                     QNYRSQGNILDAANALIRNNHARLGKNLWTAQGNGEPLRLYEAMTDLEEAGYILEEVK
                     QLKNEGTALSDVALLYRSNAQSRVLEHALFNAGISYRVYGGLRFFERQEIKHALAYLR
                     LVANPQDDGAFLRVVNFPTRGIGNRSIEQLQNLATGSGVSLWQAACSNSLSGKAGSSI
                     TGFVRLIESMRSACEALPLPETVDHVVELSGLNAYYRNEREGEDRLENLGELTNAAMA
                     FVSADGPIALVNEEGEIQSQGFPALADFLAHASLEAGEHQAAEGADALQLMTVHSAKG
                     LEFHAVFITGLEEGLFPHENSISEQDGLEEERRLMYVALTRARRRLYLTLAQTRMLHG
                     QTRYNIASRFLEEIPEALIRRVNTTKFSGFVSARGFDPASSAPRSPAEREVSGWRVGQ
                     NVVHAKFGAGVIINAEGRGADARVQVNFRTGGMKWLALEYAKLAPA"
     gene            complement(2268..3146)
                     /locus_tag="VN496_09345"
     CDS             complement(2268..3146)
                     /locus_tag="VN496_09345"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HXF17198.1"
                     /translation="MSSKHPIIAVTGSSGAGTTAVRRTFEAIFRLEHIAAALIEGDSF
                     HRYDRDEMRKLIEAYEHMGGQAISHFGPEANLFEELDALFGEYSRSGGGTSRLYLHNE
                     ERAARYGRRAGTFTAWEALPENTDVLFYEGLHGGIVTDKVNVAAHVDLLIGVTPTVNL
                     EWIQKIHRDMQGRGYSIEAVTQTILRRMYDYVHYITPQFSRTHVNFQRVPTVDTSNPF
                     VVRDVPRPEESFVVIRLADPEHLNVPYLLAMIDDSFVSRPDTIVVPGTKMEYAMQLIL
                     TPMIRDLVSASRKAKG"
     gene            complement(3243..4133)
                     /locus_tag="VN496_09350"
     CDS             complement(3243..4133)
                     /locus_tag="VN496_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXF17199.1"
                     /translation="MIKAGTITVRSGLFFILLVATGLSHAAPDLLVEGVRLPAWVIRD
                     GNRQPLALAATLKKNDQISTGNSARVLLRLGDGSMVKLGENANLEVSDLLQRPQDRFI
                     AATLKVLEGAFRFTTGTSVKYRTRREINVRFPTLTAGIRGTDIWGKKLGDREVIVLIE
                     GKITVTRAGDRPVQMNDALTYLQAPKSGATTIAAVLKDQLKAWAEETELGEAHGSLRK
                     GGRWKLYIGSYREQLEALSLYDSLRREGYPVRVLPQQTEDGNRYRLRLAGFPSEQDAE
                     AVGMRLKAFNPNIESVASLL"
     gene            4232..>5020
                     /locus_tag="VN496_09355"
     CDS             4232..>5020
                     /locus_tag="VN496_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXF17200.1"
                     /translation="MHPQYEPAAVEQPLQKEWADKRSYRALELPHKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRYHRMRGFNVLQPMGWDAFGLPAENAAMANQVPPAKWTY
                     DNISYMKKQLKSLGFAIDWERELATCTPQYYRWNQWLFLRMLAKGIAYKKTGTVNWDP
                     VDQTVLANEQVIDGRGWRTGAPVVKREIPMYYLAITKYSEQLLSSLDSLRDWPERVKT
                     MQANWIGKSFGVRVAFPYVLDGEPEKLWVFTTRADTIMGTTFCAV"
BASE COUNT         1037 a         1443 c         1443 g         1097 t
ORIGIN      
        1 cattcccgcc ccaggcgcga cgcagtaaaa aaatcaggcg ggtgccagct tggcatattc
       61 aagtgcgagc catttcatgc cgccagtgcg gaaattgacc tgaacgcggg catcggcccc
      121 gcggccttcc gcattaatga ttacgcctgc gccgaacttg gcgtggacga cattttgtcc
      181 aacacgccag ccggacactt ctctttccgc aggagaccgt ggtgcgctag acgccggatc
      241 aaagccccgc gcactgacaa acccggagaa cttggttgtg ttgactcgcc tgatcagtgc
      301 ttcaggaatt tcttccagga acctcgacgc aatgttatag cgagtttgcc catgcagcat
      361 acgggtctgc gccagtgtca gatacagccg gcggcgcgcg cgtgtcagcg ccacgtacat
      421 cagccgcctt tcctcttcca atccatcctg ctcggagatg ctgttctcgt ggggaaacaa
      481 accttcttcc agtccggtga tgaaaacagc atgaaattcc agccctttgg ccgaatggac
      541 ggtcatcagc tgcaatgcat ccgcgccttc cgcggcttga tgttcgccgg cttccagtga
      601 agcatgcgca agaaaatccg ccagcgccgg gaagccttgc gattggatct cgccctcttc
      661 gttaaccagg gcgatcggtc cgtcggcaga tacgaaagcc atggccgcgt tggtgagttc
      721 gccaagattc tcgagacgat cctcaccctc ccgctcattg cggtagtagg cgttgaggcc
      781 gcttagctcg acaacgtggt caactgtttc cggcaatggc aaagcctcgc atgccgatcg
      841 catcgattcg atcagccgca caaaaccggt gatgctgctg ccggccttgc cactcaggct
      901 attggagcaa gccgcctgcc acagcgagac gccgctgccg gtggccagat tctgcaattg
      961 ttcgatactg cgattgccga tgccgcgggt tgggaaattg acgactcgca ggaaggcgcc
     1021 gtcgtcttgc gggttcgcga ccagccgcag ataggccaac gcatgcttga tttcctggcg
     1081 ttcgaaaaaa cgcagaccgc cgtagacccg atacgagatg ccggcattga acaatgcgtg
     1141 ctcgagaaca cgcgactgtg cattggaacg ataaagtagc gcgacgtccg aaagcgccgt
     1201 gccctcattc ttcagctgct tgacctcctc cagaatatac ccggcttctt cgagatcggt
     1261 catggcctcg tagagccgta acggctctcc gttgccttgt gccgtccaca gattttttcc
     1321 aaggcgagcg tggttgttcc ggatcaatgc attcgcggca tccagaatgt tgccctggct
     1381 gcgatagttc tgttcgagct tgattacttt ttcgacatga aagtcctttt cgaaatcaag
     1441 catgtttccc gcgcgcgcgc cgcgaaatgc gtaaatcgac tggtcatcgt ccccgaccgc
     1501 gaaaatgaca ttgctcgggc cagcgagcag cttcagccat agatactgca agcggttcgt
     1561 gtcctggaat tcatcgacca gaatatgtct gaagcgcgcg cgatagtgct cccgcaagac
     1621 ttcgttcttc gcaagcagct cgaaacagcg cagcagcaac tcggcgaaat ccaccgcgcc
     1681 ttcgcggttg cactgcgtgt cataggcatc atagacagtc gccagcttgc gggaaaaatc
     1741 gtcgaacgcc tctacctttg cggcgcgcaa accctggtcc tttgcggcat tgatcgtgta
     1801 ttgcgcctgt ttcggcgggt acttttcatc atcgatattc atggatttca tgacccgctt
     1861 gatcatggcc agctggtcgg cggagtcgag aatctggaac gtttgcggca atccggcatc
     1921 ccggtgatgc gcccgcaaaa tacgattgca cagtccatgg aatgtgccga cccacatgcc
     1981 gcgcgtgttg attggcagca ttgccgaaat gcgcaataac atttcgcggg cggccttatt
     2041 ggtgaaggtt accgcgagca caccctgcgg actgagctgc ccggtctgga tgagccaggc
     2101 tatgcgggtg gtgagcacgc gagtctttcc cgaaccagcg cccgcgagga tcagtgcgga
     2161 ctggtgtgga agcgtgaccg cttcgagctg ctgcggattg aggccttcga gaaaggacat
     2221 gcgcgggaat tacggattca attgcgaatt ataaatgcct tgccaggtta gcctttcgcc
     2281 tttctgcttg cgctgaccag atcgcggatc atcggcgtca gaatcagctg cattgcatac
     2341 tccattttag tacccggtac cacgatggtg tccggccgcg acacaaagga atcgtcgatc
     2401 atggccagca aataagggac attcaggtgt tccggatcgg ccaggcgaat aacgacaaag
     2461 ctttcctctg gccggggaac gtcacgcacg acgaagggat tggacgtatc gaccgtcggc
     2521 acgcgctgga agttgacatg agtgcgcgag aattgcggcg tgatgtagtg cacgtagtcg
     2581 tacatgcgcc gcaggatggt ctgcgtcacc gcctcaatgg aatagccgcg tccctgcatg
     2641 tcgcgatgta tcttctggat ccactccaga tttactgtcg gagtgacacc gatgagcagg
     2701 tcgacatgcg cggcgacgtt gaccttgtcg gtcacgatgc caccgtgcag gccttcataa
     2761 aacagcacat ccgtattctc tgggagcgct tcccacgcgg taaaggttcc ggccctgcgg
     2821 ccgtaacggg cggcgcgctc ttcgttgtgc aaataaagcc gcgaggtgcc accaccgcta
     2881 cggctgtatt cgccaaacaa agcgtccagt tcttcgaaca ggtttgcttc ggggccgaag
     2941 tggctgattg cctggcctcc catgtgttcg taggcctcga tcagcttgcg catctcatca
     3001 cgatcatagc gatggaagct gtcaccctcg atcagtgctg ccgcgatgtg ctcgagacgg
     3061 aagatcgcct cgaatgtcct gcgcaccgcg gttgtccctg cgccggagga gccggtaacg
     3121 gcaatgatgg gatgcttgct cgacatgatg aaccgggcgg gtaggcgcgc attatgaaaa
     3181 tcgctggcgc cgatggtaca tcgaaatgcg gcgatgccga ttcgccgtgg aggcgcgccg
     3241 atctatagca gcgaagcgac ggattcgata ttgggattga atgctttcag gcgcattccg
     3301 accgcttctg catcctgttc gcttgggaat ccggccagac gcagccgata acggttcccg
     3361 tcttcggttt gctgtggcag gacccggacg ggataccctt cacgccgcaa actgtcatac
     3421 agcgacagcg cttcgagctg ctcgcggtag ctgccgatat aaagtttcca gcgtcctcct
     3481 ttgcgaaggc taccatgggc ttctcccagc tccgtttcct cagcccaggc cttcagctgg
     3541 tcctttaaca ctgccgcgat cgttgtggcg ccgctcttgg gtgcctgaag ataagtcaag
     3601 gcatcgttca tctgcaccgg ccgatcgccg gctctggtca cagtaatctt gccctcgatc
     3661 aacacgatca cttccctgtc accgagtttc ttgccccaga tatcggtacc gcggatgccc
     3721 gcggttagtg tcgggaaccg gacgttgatt tctcgcctgg tgcgatattt caccgaagtg
     3781 ccggttgtaa agcggaacgc gccttccagt actttaagcg tcgcggcgat gaagcgatcc
     3841 tggggccttt gcaggaggtc tgacacttca aggttggcat tttcgcccag cttgaccatg
     3901 cttccgtcgc cgagtcgcaa gagcacgcgg gcgctgtttc cggtcgagat ttggtcgttc
     3961 tttttgagcg tggcggcgag cgcaagaggc tgccggtttc cgtcccgaat aacccaggcc
     4021 ggcaaacgga caccttcgac gagcaagtcc ggggctgcat gcgacagacc ggtcgctacc
     4081 agcaagatga agaacaaccc gcttcgtacg gttatcgtgc cggcctttat catttgcatt
     4141 tgagtcacgc tgcaagatac gggctgaagc ggcgaatgga tgcgctggcg acggtataat
     4201 cggttacttt acagcgcttt gtcacatcgt catgcacccg caatacgaac ccgcggcagt
     4261 ggaacaaccg ctccagaaag aatgggcaga caagcgatcc taccgggccc tggagctgcc
     4321 gcataagccg aagtactact gcttgtcgat gtttccttat ccgtccggca aattgcacat
     4381 ggggcatgtg cgcaactaca ccatcggaga cgtcctttcg cgctatcacc gcatgcgcgg
     4441 tttcaatgtt ctgcaaccga tgggctggga cgcattcggc ctgcccgccg aaaatgcggc
     4501 gatggcaaat caggtgcctc ccgcgaaatg gacgtacgac aacatttcgt acatgaagaa
     4561 gcagctcaag tcgcttggat ttgcgatcga ctgggagcgg gaactggcga catgcacccc
     4621 acaatactat cgctggaatc agtggctgtt cttgcgcatg ctggcaaagg gaattgccta
     4681 taagaaaacc ggtaccgtga actgggatcc ggtggaccag actgtgcttg ccaatgagca
     4741 ggtcatcgat ggtcgcggct ggcgcaccgg cgcgccggtc gtgaaacgag agatcccgat
     4801 gtattacctc gcgatcacga agtattcgga gcagttgctg tcttcactcg attcactgcg
     4861 ggactggccg gagcgcgtga agaccatgca ggcaaactgg atcggaaaaa gcttcggcgt
     4921 gcgcgtggct tttccgtacg tgttagatgg cgagccggaa aagctatggg ttttcacgac
     4981 gcgggcggac accatcatgg ggacaacatt ttgtgcggtg
//
LOCUS       DATMZQ010000288         4791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1963637, whole genome shotgun sequence.
ACCESSION   DATMZQ010000288 DATMZQ010000000
VERSION     DATMZQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963637"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VN496_09360"
     CDS             <1..289
                     /locus_tag="VN496_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF17201.1"
                     /translation="PAESIFQACLLRFRPIMMTTLSALLGALPLMIGSGVGSELRHPL
                     GITMVGGLLFSQALTLFTTPVIYLAFDRLANRGARPVSDASDIPHPRSAEP"
     gene            286..3393
                     /locus_tag="VN496_09365"
     CDS             286..3393
                     /locus_tag="VN496_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXF17202.1"
                     /translation="MKFFELFIHRPVATVLLTLGIALSGVIAFRLLPVSPLPQVDFPT
                     ISVQASLPGASPETMAATVATPLERALGRIAGITEMTSSSSLGTTRVTLQFELSRNID
                     GAARDVQAAINSARALLPTGLPSNPTYRKVNPADSPVMILSLTSDSLTRGQMYDAASS
                     ILAQKLSQIDGIGQVSVGGSSLPAVRVELIPSALHKYGIGTEDVRTALAAANANRPKG
                     MVEEGARHWQISANDQAKQAAEYLPLIVAYRNGAAVHLADVADVTDSVQDLRNAGSAN
                     GRPAVLVILYRQPGANIIETVDRVRAILPVLAASIPSAIDLSVVIDRTPTIRASLHEV
                     ERTLMISIALVVMVVFVFLRKASATLIPSIAVPVSLLGTFGVMYLVGYSLNNLSLMAL
                     TVATGFVVDDAIVVLENISRRVEEGVAPLQAALQGTREVGFTVLSMSISLIAVFIPIL
                     LMGGIIGRLFREFAVTLSVAILVSLVLSLTVTPMICARWLRPGEPRADPGRISQFSQR
                     AFASLLQGYERSLAWALAHSRFMMLLLAATICLNVYLYVVVPKGFFPQQDTGRLNGFI
                     QADQAISFQAMQKKLSDFIEIVRRDPAVENVIGFTGGGQRNSGAMFISLKPLAERKLS
                     VDRVIARLRGKLAAEPGASLFLQAAQDIRMGGRASNAQYQYTLQADDLAELRTWEPRI
                     RQALMPLPQLADVNTDSQDKGIQTSLSIDRDAASRLGVSARVIDATLNDLFGQREVST
                     IYTALNQYRVVMEAAPPYWQSPEALNDVFVSNADGKQVPLAAFASYAPTSTPLAVNHQ
                     GQFVASTISFNLPVGVALSDATNAINDTMRRMGVPTSVQGSFQGTARAFQASLNSQPW
                     LILTALLAVYIVLGILYESLVHPLTILSTLPSAGVGALLALLAFDIELSIIALIGVIL
                     LIGIVKKNAIMMIDFALDAERRHGMSPEQAIYRACLLRFRPIMMTTMAALLGAIPLAI
                     GFGDGSELRRPLGISIVGGLIVSQMLTLYTTPVVYLYLDRFRLWSKRKWSGSATAGQT
                     IAEAG"
     gene            3390..>4791
                     /locus_tag="VN496_09370"
     CDS             3390..>4791
                     /locus_tag="VN496_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXF17203.1"
                     /translation="MKPVHDRFAALAAVLVLLAACTVGPDYVRPTADTPVAFKESQGW
                     KTAQPRDQELRGSWWEAFNDPLLNEMMEQVNLSNQNLVQAAAQFRQARALVQSARAGY
                     LPSVSANAGVTRSSSSLTSRNTGSTQNPGFSATSYSLSLDALWEADLWGRVRRTVEAN
                     STSAQASAADLDALRLSIRAELAQDYFQLRALDTQQKLFEDTVAAYQRTLVLTQNQYA
                     AGVAAKVDVIQAQTQLKTTQAQAIDLGVLRAQLEHAIALLLGKPASNYSLPPAPLAAV
                     APAVPVGVPSSLLERRPDIAAAERRMASANAQIGVAEAAYYPSLTISASGGFQSTSFS
                     NWLTAPSRFWSLGPALAQTLFDGGLRRAQTDQAIAAYDANVAAYRQTVLTGFKEAEDN
                     LAALRILEEEAAVQEEAVQNARQSVSLTTNQYKAGIVSYLNVVVVQAAALNNERAAVD
                     ILNRRLAASVLLIKALG"
BASE COUNT          877 a         1516 c         1450 g          948 t
ORIGIN      
        1 cccggcggag tcgatctttc aggcttgcct gctgcgcttt cggccgatca tgatgacaac
       61 cctgtccgcg ctgctcggcg cgttgcccct gatgatcggc agcggcgtgg gttctgaact
      121 gcgccatccg ctcggcatta ccatggtggg cggactgttg ttcagccagg cgttgaccct
      181 gttcacaacg ccggtgattt acctggcgtt cgaccggctg gccaaccgag gcgcgcgacc
      241 tgtttcggac gctagcgata ttccgcatcc gcggagcgcg gagccatgaa attcttcgaa
      301 ctattcatcc accggccggt ggccaccgtg ctgctcacgc tcggcatcgc tttgtccggt
      361 gtgattgcgt ttcgcctgct gccggtctcg ccattgccgc aagtggattt tccgaccatc
      421 tcggtgcagg cctcgttgcc cggcgcaagc ccggaaacga tggcagccac cgtggcgaca
      481 ccgcttgaac gcgcgctcgg ccgcatcgcc ggaattaccg aaatgacctc gtccagttcg
      541 ctcggcacga cgcgcgttac tttgcagttc gaactgtcgc gcaacatcga tggggcagca
      601 cgcgacgtac aggcagccat caattctgcg cgcgctctgc tacccacggg gctaccgagc
      661 aaccccacgt atcgcaaggt caaccctgcc gattcgccag tcatgatcct gtcgcttacc
      721 tcggacagct tgacgcgggg ccagatgtac gacgcggcct ccagcatcct ggcacagaag
      781 ctgtcgcaaa tcgacggcat cgggcaagtt tcggtcggcg gcagttcgct gccggcagtg
      841 cgggtggaac ttattccatc ggcgctccac aagtacggca tcggcacgga agatgtccgc
      901 actgcgctgg cggcagcgaa tgccaaccgc cccaaaggca tggtcgagga aggcgcacgg
      961 cactggcaga tttctgccaa cgaccaggcc aaacaggctg ccgaatatct gccgctgatc
     1021 gtcgcttacc gcaacggcgc ggcggtgcac ttagcggatg tcgccgacgt gacggactcg
     1081 gtgcaggact tgcgcaatgc cggatcggcc aatggccggc ctgcggtact cgtcattctg
     1141 taccggcagc ccggggcaaa tatcatcgag accgtggacc gggtccgcgc catcctgccg
     1201 gtcctcgcgg cttccatccc gagcgcgatc gatctttccg tcgtcataga ccgcaccccg
     1261 accatacgcg cgtcgctgca tgaagtggag cgtacgctga tgatctcgat tgcactggtc
     1321 gtcatggtcg tgttcgtgtt tctgcgcaaa gcgtccgcca cgctgatccc cagcatcgcc
     1381 gttccggtgt ccctgctcgg cacattcggc gtcatgtacc tggtgggcta cagcttgaac
     1441 aacctgtcgc tgatggcgct gactgtcgcg accggttttg tggtggatga cgcgatcgtc
     1501 gtcctggaaa acatatcgcg acgcgtcgaa gaaggcgtcg cacccctgca ggcagcgctg
     1561 cagggcacgc gcgaggtcgg cttcaccgtg ctgtccatga gcatatcgct gattgccgtt
     1621 ttcattccga ttctgctgat gggaggcatt atcggccggc tgtttcgcga attcgcggtg
     1681 acgctgtccg tcgccattct ggtctcgctg gttctttcgc tgacggtgac gccgatgata
     1741 tgcgcgcgct ggttgcgtcc gggcgaaccg cgcgcagatc cgggccggat atcgcaattc
     1801 agtcagcgcg cgttcgcttc gctgttgcag ggctacgaac gctcgcttgc atgggcgctg
     1861 gcgcacagcc gcttcatgat gctgctgcta gcggcgacga tctgcctgaa cgtttatctg
     1921 tatgtggtcg tgccgaaggg atttttccca caacaggata ccggccgact caacggattc
     1981 atccaggccg atcaggccat ctcgtttcaa gccatgcaga aaaagctaag cgacttcatc
     2041 gaaattgtgc gccgggaccc ggctgtcgag aatgtcatcg gctttaccgg tggcggacag
     2101 cgcaacagcg gcgccatgtt catctcgttg aagccgctgg ccgagcgcaa gctgtctgtg
     2161 gaccgggtga ttgcgcgctt gcgcggcaaa ctggcggctg aaccgggtgc aagtttgttt
     2221 ctgcaggcgg cgcaggatat tcgcatgggc ggacgcgcat ccaatgccca gtatcagtac
     2281 acgctgcagg ccgacgacct tgccgaactg cggacttggg agccacgcat acgccaggca
     2341 ctgatgccat tgccgcagct ggccgacgtg aacacggaca gtcaggacaa aggaatacag
     2401 acttcactgt ctatcgaccg cgacgcggcc tcccgcctcg gcgtcagcgc gcgcgtcatc
     2461 gatgccaccc tcaacgacct gttcggccag cgggaagtat cgaccatcta tacggcgctg
     2521 aatcaatatc gcgtcgtgat ggaagccgcc ccgccctact ggcaaagtcc cgaggcgttg
     2581 aatgacgtct tcgtatccaa tgccgatggc aagcaagtgc cgctggccgc cttcgcaagc
     2641 tatgcaccga ccagcacacc gctggcggtg aaccatcaag gccagtttgt ggcctcgacg
     2701 atttctttca atcttcctgt tggcgtagcg ctgtcggatg caaccaacgc aatcaatgac
     2761 accatgcgac gcatgggcgt accaacttcg gtccagggca gcttccaggg cacggcacgt
     2821 gcttttcagg cgtcattgaa cagccagccc tggctgattc tgacggcgtt gctcgcggta
     2881 tacatcgttc tcggcattct ctatgaaagc ctggtgcatc ccctgaccat tttgtccacg
     2941 ctgccctctg ccggcgttgg cgccttgctt gcgctgctgg cattcgatat cgaactcagc
     3001 atcatcgcgc tgatcggcgt catcctgctg atcggcattg tcaagaagaa tgcgatcatg
     3061 atgatcgact tcgcgctcga tgccgagcga aggcacggca tgagcccgga gcaagccatc
     3121 taccgagcct gcctgctgcg ttttcgccca atcatgatga cgaccatggc ggcgctgctg
     3181 ggcgcgattc cgctcgcgat cggattcggc gacgggtccg agttgcgccg tccgctcggc
     3241 atctcgattg tcggcggcct gatcgtcagt cagatgctca cgctctacac cacaccggtg
     3301 gtttatttat atctcgaccg cttccgcctc tggagcaagc gcaagtggtc aggctcggca
     3361 acggctggcc agactatcgc ggaggccggt tgaaacccgt tcacgacaga ttcgctgcgc
     3421 tggcggcagt gttggtactg cttgctgcat gcaccgttgg tccagactac gtgcggccca
     3481 cggccgatac gccggtcgcg ttcaaggaat cgcaaggctg gaaaacggct caaccgcgcg
     3541 accaggagtt gcgtggcagc tggtgggaag ctttcaacga cccgctgctc aacgagatga
     3601 tggagcaggt caacctgtcc aaccagaatc tggtgcaggc cgccgcgcaa ttccgccagg
     3661 cgcgggcact ggtgcaatct gcacgggcag gttatctgcc cagcgtatcg gcgaacgccg
     3721 gggtcacgcg ttcttcgtcg agcctgacga gccggaacac cggatcgacg caaaatccgg
     3781 gattttcggc gaccagctat tcgctgtcgc tggatgccct ctgggaagct gatctatggg
     3841 ggcgcgtgcg ccgcacggta gaagcaaact caaccagcgc acaggcgagc gcggccgatc
     3901 tggatgcact gcgcctgtcg atccgtgccg aactcgcgca agactatttc cagctgcgcg
     3961 ctctcgatac gcagcagaag ttgttcgaag acacggtcgc cgcgtaccag cgcacgctgg
     4021 tcctgaccca gaaccagtac gcggcgggtg tcgcggccaa agtcgatgtg atccaggccc
     4081 agacgcagct caagaccacg caggcacaag ccatcgatct cggcgtactg cgcgcacaac
     4141 tcgagcacgc gatcgcgctg ttgctcggca agccggcctc caactattcg ttgccacctg
     4201 cgccgcttgc cgctgttgca cccgcagttc cggtcggcgt gccgtccagt ctgctcgagc
     4261 gccgtccgga catcgcggct gcggagcgac gcatggcgtc tgccaacgcg caaattggcg
     4321 tagccgaggc cgcctactat ccttcactga caatttcagc ctcgggtggc ttccaaagca
     4381 cgagtttttc gaactggctc acggcaccca gccgtttctg gtcgctcggt ccggcgctgg
     4441 cccagaccct gttcgatggc gggttgcgcc gcgcccagac tgaccaggcc atcgcggcat
     4501 acgacgccaa cgtagcggct taccggcaaa cggtgctcac cggattcaag gaagccgagg
     4561 acaatctggc ggcgctgcgc attctcgaag aagaagcagc cgtgcaggag gaagcagtgc
     4621 agaacgcgcg ccagtcggtg tcgctgacca ccaaccagta caaggcgggc atcgtcagct
     4681 atctgaacgt cgtggtggtg caggcagccg cactgaacaa tgaaagagcc gcggtcgata
     4741 tcctgaaccg gcggctggcc gcgagcgtgc tgttgatcaa agcattgggt g
//
LOCUS       DATMZQ010000289         5143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2999369, whole genome shotgun sequence.
ACCESSION   DATMZQ010000289 DATMZQ010000000
VERSION     DATMZQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999369"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VN496_09375"
     CDS             complement(<1..151)
                     /locus_tag="VN496_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HXF17204.1"
                     /translation="MTQLNDLPFSVLQFYATAPYPCSYLPDKLARSQVATPSHAIDSQ
                     VYSELV"
     gene            complement(162..854)
                     /gene="aat"
                     /locus_tag="VN496_09380"
     CDS             complement(162..854)
                     /gene="aat"
                     /locus_tag="VN496_09380"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXF17205.1"
                     /translation="MIPWLRSGDPFPPVEEAAAEPNGLLAAGGDLSVARLVDAYRSGI
                     FPWFNEGQPILWWSPDPRMVLFPAELKISRSLTKTLRHSRFEIRVDTAFRDVILNCGM
                     PRRDQHGTWITDRMVEAYCEMHRHGIAHSVETWLEEELVGGLYGVSLGHAFFGESMFT
                     RVTDASKVALVTLVRQLELWGFGLVDCQMNTAHLASFGAREIPRAEFTRRLRELIHYP
                     TIPAPWQLAPVR"
     gene            complement(867..1409)
                     /locus_tag="VN496_09385"
     CDS             complement(867..1409)
                     /locus_tag="VN496_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXF17206.1"
                     /translation="MKSVWILICGAVLVAGCSATKDFFSKKQSATAVLAPTKGSSVSG
                     TVNFTQKGNMVLVDAKVNGLKPDSASGIHIHEKGNCSAADASSAGGHFNPSASQHGGT
                     SGTIRHGGDLGNLKADANGSAQTSIEVGGISLGTGPDSIIGKSVIVHAGTDDLKTQPS
                     GNSGARAACGLISKNPDKVF"
     gene            1555..2124
                     /locus_tag="VN496_09390"
     CDS             1555..2124
                     /locus_tag="VN496_09390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF17207.1"
                     /translation="MANTHAEHSADQPSDWVARWIENIRPGGAVLDLACGSGRHSRLL
                     ASQGFQVWAVDNDDLALQGLHGRAGITTLLADLEKDPWPLQNSRFDGVVVTNYLHRAL
                     FPHILDALAAAGVLIYETFAVGNERFGKPSNPDFLLRPGELLEVARTRLRVVAYEDLE
                     VTAPKPARVQRICGCNIEQRDMPAPERIS"
     gene            2156..3064
                     /gene="dapA"
                     /locus_tag="VN496_09395"
     CDS             2156..3064
                     /gene="dapA"
                     /locus_tag="VN496_09395"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXF17208.1"
                     /translation="MLKGSLVAIVTPMFEDGSLDLQGLRALIDFHVREGTNGIVVVGT
                     TGESPTVDFEEHCLLIRTAIDQAAGRIPIIAGTGANSTREAIELSAYAKQVGADMSLS
                     VVPYYNKPTQEGLYRHFRAIAEAVELPLILYNVPGRTVADMQNDTAIRLAQIPNIVGI
                     KDATGNMERGAELLRRAPKNFAVYSGDDGTGLALLMLGAHGVISVTANAAPRLMQEMC
                     AAVLSGEFAKARAFNNKLLGLHRHLFVEANPIPLKWVLHQMRLMEPGIRLPLTPLSSS
                     FHDLLRGAMEQADIQVSSAFTPALAK"
     gene            3110..4252
                     /gene="bamC"
                     /locus_tag="VN496_09400"
     CDS             3110..4252
                     /gene="bamC"
                     /locus_tag="VN496_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HXF17209.1"
                     /translation="MKDNARMNASPLICAAIAAAIAVAGCSGSVIEGKKIDYKSAGKV
                     KPLDVPSDLSKPPGSDRYNIPEPNAGSATASEYAEGQGTRVAAGPTPVLPKQEKISFK
                     RAGSQRWMSIEADPEALWPVIKDFWLDNGFLINVESRPTGIMETDWAENRAKIPDSVI
                     RSLIGKVLDSAYSYPERDKFRTRLERGENAATTEIYISHRGMAQINVSKDRYTETTVW
                     QVRPVDPDLEAEMLYRLAIRLGAREEQIKADAKAPDEPKATITKSGELPDALLLVDGF
                     DRAWRRVGLALDRVGFTVEDRDRSKGTYFVRYVDADAKDSDKSDGFWSKLAFWRDSGP
                     KKDEQYRISVAEATKGSEVKVLDAKGAQSTSPTAGRILALLQQELK"
     gene            4286..5050
                     /locus_tag="VN496_09405"
     CDS             4286..5050
                     /locus_tag="VN496_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF17210.1"
                     /translation="MRFASLGSGSEGNALVVEAQGTRVLVDCGFGLAATEARLGRLGL
                     VPEDVSAIVVTHEHDDHIGGVSRLARRHGIPVWITPGTLAGLEPLFDDVDVRPICNYE
                     IFSVGHLQVQPFPVPHDAREPAQFVFGDGACRLGVLTDVGCSTRHIESVLSGCDALVL
                     ECNHDSDMLRTGSYPQKLRDRIAGRFGHLDNEAAAGLLGKLDNGKLKHLVAAHLSKEN
                     NHPELARAALAAVLNCTPGWIDIADQDQGLDWRDIR"
BASE COUNT         1119 a         1445 c         1528 g         1051 t
ORIGIN      
        1 tgaccagttc gctgtagacc tggctgtcaa tggcatgact cggtgttgca acctgcgagc
       61 gcgcaagctt gtcgggcagg tagctgcacg gatagggcgc agtcgcataa aactgcaata
      121 ccgagaaagg caggtcgttg agttgtgtca tcgccgttga tctatcgcac tggtgcaagc
      181 tgccacggag ccggaatagt tggatagtgt atcaattctc gcaggcgccg cgtgaattcg
      241 gcgcgcggta tctcgcgagc tccaaagctg gccagatggg cggtgttcat ctgacagtcg
      301 accagtccga agccccagag ttcgagctga cgtaccaatg tgacaagcgc gactttggac
      361 gcgtccgtga cacgcgtgaa catcgattcg ccgaaaaacg cgtggccaag cgaaactcca
      421 taaagaccgc ccaccaattc ctcttccagc caggtctcca cgctatgcgc aatgccatgg
      481 cgatgcatct cgcagtaggc ttcgaccatc cgatccgtga tccacgtgcc atgctgatcc
      541 cggcgcggca tgccgcaatt cagaataacg tcccggaaag ccgtatcgac ccggatttca
      601 aaccgggaat ggcgcagcgt cttggtcagc gagcgggaaa ttttgagctc cgccggaaac
      661 aggaccatcc gcggatctgg actccaccag agaatcggct ggccttcgtt aaaccaagga
      721 aagatgccgc ttctgtaggc atccaccaaa cgcgcaaccg aaaggtcgcc gccggccgca
      781 agcaatccat tgggttcggc cgccgcttct tcgaccggcg ggaaaggatc gccggagcgc
      841 agccacggaa tcaagtgacg ttcagctcag aagaccttgt ccgggttctt gctgatcaga
      901 ccgcaagccg cgcgagcacc ggaatttcca gaaggctgcg tcttgaggtc gtccgtgccg
      961 gcatgcacga tgactgactt accaatgatg ctgtctggcc cagtgccgag ggatatcccg
     1021 ccgacttcaa tggacgtctg tgcagaacca tttgcgtctg ctttcaggtt gccaaggtcg
     1081 cccccatgcc ggatcgtgcc cgatgtgcca ccgtgctggg aagccgaggg gttgaagtgt
     1141 ccaccggcgc tggaagcgtc cgccgcgctg caattgccct tctcgtgaat gtgaatgccg
     1201 ctggctgaat ccggtttcaa tccgtttacc ttggcgtcca ccagaaccat gttgcctttt
     1261 tgcgtaaaat tgaccgtgcc gctgactgaa ctgcccttgg tcggcgcgag caccgcagtc
     1321 gcagactgct ttttactgaa gaagtcttta gtagcgctgc agcccgccac caatacagcg
     1381 ccacagatca aaatccagac ggatttcatt tccttctccc gttattgcat tggctcatca
     1441 gtcggtgcac ctacaaaaca ggcaggtttt tagcactcgc ccagtgaggt gtcaaatcag
     1501 ctcagtgaaa acagtatcgc gggcctcgcc ttgtgcttct gtaaaatcgc gccaatggcc
     1561 aacacgcatg cagagcattc agcggatcaa ccctccgact gggtagctcg ctggatcgaa
     1621 aatatcagac cgggtggagc ggttctggat ctcgcttgtg gcagtggacg ccatagccgc
     1681 ttgctggcgt cgcagggttt tcaggtgtgg gcggtcgata acgacgacct ggcgctgcag
     1741 ggtttgcatg gcagggccgg gatcactacg ttgttggccg acctggaaaa agacccgtgg
     1801 ccattgcaaa actccagatt cgatggcgtg gtcgttacga attacctgca ccgcgcgttg
     1861 tttccgcaca tactcgacgc gcttgcggcc gccggcgtgc tgatttatga gacattcgct
     1921 gtcggaaacg aacgctttgg caagccttcg aatccggatt ttttgctgcg acccggtgaa
     1981 ttgctcgaag ttgcccgcac tcgcttgcgt gtagtcgctt acgaggatct cgaagtgact
     2041 gcgccgaaac ctgcgcgagt acagcgcatt tgcggctgta acatcgaaca gcgtgacatg
     2101 cctgcgccgg aaagaatcag ttagaatccg ggctttcttg ttgaggaaac tcgccatgct
     2161 aaagggcagc ctcgtcgcca tcgtgacacc aatgttcgag gatggcagtc tggatctcca
     2221 gggactgcgg gccctgatcg atttccatgt tcgcgaaggc accaacggca tcgttgtcgt
     2281 cggcacaact ggcgaatcgc ctacggtgga ttttgaagag cactgcttgc tgattcgcac
     2341 cgcgatcgac caagctgcag ggcgcatacc gattattgcg ggcactggcg caaactcaac
     2401 tcgcgaggca attgaactgt ccgcgtacgc caagcaagtt ggagcggaca tgagcctgtc
     2461 cgtcgtaccg tattacaaca agccgactca ggaaggactg tatcgtcatt tccgcgcgat
     2521 cgcggaagcc gtggaattgc cgctgattct gtacaacgtg ccgggacgca cagtcgctga
     2581 catgcaaaac gatacggcga tacggctcgc gcaaattccc aacattgtcg gcatcaagga
     2641 tgccaccggc aacatggaac gtggcgccga gctattgcgg cgtgcaccga aaaatttcgc
     2701 ggtatacagc ggtgatgatg gcaccgggct cgcattgctg atgctgggcg cgcatggcgt
     2761 tatttcggtt acggccaatg ccgcgccccg tctgatgcag gaaatgtgtg cggcggtatt
     2821 gtccggagag tttgccaagg cacgtgcatt caataacaag ctgctcggcc tgcatcgcca
     2881 cctgttcgtc gaagcaaatc cgattcctct gaagtgggtc ctgcatcaaa tgagactgat
     2941 ggagcccggc atccgattgc cgctgacgcc gctttcttcc tccttccacg atttgttgcg
     3001 cggtgcaatg gagcaagccg acatccaggt ttcttctgcg ttcactccag cattggccaa
     3061 gtgagtagtg ggccagcgaa acgttgcgac cgtccgggtc gcgatccgaa tgaaagataa
     3121 tgcacgcatg aatgcatcgc cgttaatttg cgccgcgatc gcggcggcta ttgcagttgc
     3181 tggttgttcc ggatcggtga tcgaaggcaa gaagatcgac tacaaatcgg ccggtaaggt
     3241 caaaccgctc gatgtcccga gcgacctatc gaagccgcct ggcagtgacc gctataacat
     3301 cccggaaccg aatgctggat cggcgacggc ttcggaatat gcggaaggcc agggcacgcg
     3361 cgtggcggca ggccccacac cggtgttgcc gaaacaggaa aagatcagct tcaagcgtgc
     3421 cggcagtcag cgctggatgt caatcgaagc cgacccggaa gcattgtggc ccgtgatcaa
     3481 agacttttgg ctggataatg gttttctgat caacgttgaa tctcggccga cgggaatcat
     3541 ggaaacggac tgggcagaga accgtgcaaa aattccggat agcgtcatac ggagtttgat
     3601 cggcaaggtt ctggacagcg cttattcgta cccggagcgg gacaagttcc gaacgcgcct
     3661 ggagcgtggt gaaaatgccg ctactaccga aatctacatc agccaccgcg gcatggcgca
     3721 aatcaatgtt tccaaggatc gttacaccga gacaaccgtg tggcaggtgc ggcccgtcga
     3781 tcccgacctc gaggccgaaa tgctgtatcg gcttgcaatt cgcctgggag cacgcgaaga
     3841 gcagatcaag gcggatgcga aggcgccaga cgagccgaaa gccaccataa cgaagtcggg
     3901 tgaacttcct gatgcgctat tgctggtaga cggtttcgac cgtgcatggc ggcgcgttgg
     3961 tctggcgctc gaccgcgttg gctttaccgt cgaagatcgg gatcgctcga aagggacgta
     4021 ctttgtgcgc tacgtggatg ccgatgccaa agatagcgat aagtcggacg gtttttggtc
     4081 gaagctggcg ttctggcgcg acagcggacc gaagaaagat gaacagtatc gcatcagcgt
     4141 agcggaagct acgaagggca gcgaagtcaa agtgctcgat gccaagggcg cgcagtcaac
     4201 ttcgccgact gccggccgca tcctggcgct gctgcagcag gaattgaaat aatccgttgg
     4261 ttaaagtgca caggctggat tcctgatgcg gttcgcgtct ctcggcagcg gcagcgaagg
     4321 caatgcgctc gtagtcgagg cccagggcac gcgagtattg gtcgactgcg gttttggtct
     4381 ggcagccact gaagccaggc ttggccggct cggcttggtt cccgaagacg tctccgcgat
     4441 tgttgtaacc catgaacatg acgaccatat cggtggcgtg tctcggctcg cgcggcgcca
     4501 tggcattccg gtgtggataa ccccgggcac cctggccgga ctggagcctc tgttcgacga
     4561 cgtcgacgtg aggccaatct gcaactacga aatcttctcg gtcgggcacc tgcaggtgca
     4621 gccgttcccg gtgccgcacg atgcacgaga acccgcacag ttcgtattcg gtgacggcgc
     4681 atgccggctc ggcgtgctca ccgatgtggg atgttcgacc cggcatatcg aaagcgtcct
     4741 cagcggatgc gacgcgctgg tgctcgaatg taaccacgac agcgacatgc tgcgcacagg
     4801 cagctatccg caaaaattgc gtgatcggat tgcgggacgc ttcggccacc tggacaacga
     4861 agccgcagcc ggcctgttgg gaaagttgga caatggcaag ctcaaacacc tggtcgcagc
     4921 ccatctcagc aaggagaata atcatcccga gcttgcgcgc gccgccctgg cggcagtttt
     4981 aaattgcacc ccgggatgga ttgatatcgc ggaccaggat caaggcctcg actggcgaga
     5041 cattcgttag ccaggtagcg cggaaaacca agtaaagccc aatcgctggt ttaccggttt
     5101 cagccagcaa tgaaagttca aaggcccagc gtcgtagctg ata
//
LOCUS       DATMZQ010000290        16239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1273644, whole genome shotgun sequence.
ACCESSION   DATMZQ010000290 DATMZQ010000000
VERSION     DATMZQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16239
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273644"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..940
                     /locus_tag="VN496_09410"
     CDS             176..940
                     /locus_tag="VN496_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXF17211.1"
                     /translation="MARVLAVTNQKGGVGKTTTTINLAASLVSAARRVLLVDLDPQGN
                     ATMGSGIDKRALRTTVYQVLLGETAIAAVRVRSQSGGFDVIPSNRELAGAEVEMVDLA
                     DRETRLKNALREIQDEYDFVLIDCPPALNLLTVNGLCAANAVMIPMQCEYYALEGLSD
                     LVQTIKKVRVSLNPTLEIEGLLRTMYDPRNMLAQQVSDQLQQHFGDKVYRTIIPRNIR
                     LAEAPSHGLPALSFDRQSKGALAYLALAGEMLGKVA"
     gene            948..1796
                     /locus_tag="VN496_09415"
     CDS             948..1796
                     /locus_tag="VN496_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXF17212.1"
                     /translation="MAKLKGLGRGLDALLHGNEEQTHNAQAELPVELLTPGRFQPRTR
                     MDPQSIADLAASIKSQGLIQPILVRTSDNGKYEIIAGERRWRASQLAGLTQVPVIVRE
                     VPDKSALAMALIENIQREDLNPLEEATGIQRLIDEFEMTHDVAAEAVGRSRSAVTNLL
                     RLLNLSKAVQDLLMEGKIEMGHARALLAINGSRQAELARQIVSDNLSVRETEQLVGAA
                     ARTTRKAVRRRPRKDRDLQALEEELSEKLGTTVAFKTLRGGRGKLTIDYASLDQLDNV
                     LKLLRH"
     gene            1913..2257
                     /locus_tag="VN496_09420"
     CDS             1913..2257
                     /locus_tag="VN496_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HXF17213.1"
                     /translation="MRTVLLWQIAVTTFLAIIAGLLTGIHAMASAALGGLVSVTAGLA
                     FALVMQSGGAQSADGTVIAALRAEATKIGVAVILLWLVLALYKDVVPIVFIGSFIVSI
                     LVFSMAFFVRDV"
     gene            2273..3082
                     /gene="atpB"
                     /locus_tag="VN496_09425"
     CDS             2273..3082
                     /gene="atpB"
                     /locus_tag="VN496_09425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXF17214.1"
                     /translation="MAEASELTPNEYISHHLTNMTSSMGQGDFWVLHVDTLVMSVLLG
                     IVSLGFLWWAVRGATAGIPGKRQAFVELAVEFVDDQVKGIFHGDRHKFISPAALTVFV
                     WVLLMNAMDFLPVDIMAWFYEDVLHLHNWRGVPTADVNTTFALALSVWLLMIYFSIAV
                     KGLGGWIHELFGTPFGNHVILWPANLLFNLVEYISKPLSHSLRLYGNMYAGEIIFLLL
                     GLWAATGLVGAVAGVFLGAGWAIFHILIVVLQAFIFMMLTVVYLSMAHESH"
     gene            3135..3398
                     /gene="atpE"
                     /locus_tag="VN496_09430"
     CDS             3135..3398
                     /gene="atpE"
                     /locus_tag="VN496_09430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HXF17215.1"
                     /translation="MQFLALIQAYTGVGIGLMIGLGAAGACIGVGIMCSRFLEGAARQ
                     PEMIPALQAKVFLLLGLIDASFIIGVGLAMLFAFGNPLLAVIR"
     gene            3430..3903
                     /locus_tag="VN496_09435"
     CDS             3430..3903
                     /locus_tag="VN496_09435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HXF17216.1"
                     /translation="MNIGLTMLSQAIAFAIFIWFTIKFIWPPLMHAIEQRQKQIAEGL
                     AAAERGKQDLELAAKRAAEELQNARVQASEIISQAEKRGAAVIEEAKSAAKAEAERIV
                     VGAKAEIEQEVFRARESLRTQVAVLAVTGAEKILKREVDAKAHAQMLSGLEAELR"
     gene            3908..4444
                     /locus_tag="VN496_09440"
     CDS             3908..4444
                     /locus_tag="VN496_09440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HXF17217.1"
                     /translation="MAELATIARPYAEAVFRLAKQTNALGAWSDALNLIATVYRDPQM
                     QAAVANPKVTPSEIERLMLAICGERIDGVARNLIQLLVHNRRLSLLTEIRALFEQLKL
                     EDEGKLDAKISSAFPMEDTQRNQVVNLLSSRFKRKINATVIVDSNLIGGIKVEVGDKI
                     WDASVRGRLQNMAAALTK"
     gene            4456..5997
                     /gene="atpA"
                     /locus_tag="VN496_09445"
     CDS             4456..5997
                     /gene="atpA"
                     /locus_tag="VN496_09445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXF17218.1"
                     /translation="MQLNPSEISELIRSKIQNLASTSEVRTQGTVVSVTDGICRIHGL
                     ADVMQGEMLEFPDNTFGLALNLERDSVGAVVLGNYEHISEGDPVKSTGRILEVPVGPE
                     LLGRVVDALGQPIDGKGPIAAKAFDVIEKVAPGVIWRKSVSQPVQTGLKAIDAMVPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGKDLLCVYVAIGQKASTVANVVRKLEEHGAM
                     AYTIVVAATASESAALQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSEAWVERFTNGAVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGVSVSRVGGAAQTKIMKRKDTG
                     GGARLALAQYRELAAFAQFASDLDESTRKQLDRGRMVTELMKQLQYQPLQVWEMALTL
                     FAANNGHFDDIDVKKALSAENSLRDFIKTKYAALALRMDKDLSGDDEKALNEAIKDWK
                     KNATY"
     gene            6055..6918
                     /gene="atpG"
                     /locus_tag="VN496_09450"
     CDS             6055..6918
                     /gene="atpG"
                     /locus_tag="VN496_09450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HXF17219.1"
                     /translation="MAGTKEIRNKIKSVQSTRKITKAMELVAASKMRRAQERMRAARP
                     YAEKIRNVAAHISYANPEYRHPFLIARDTVKRIGIIVITTDKGLCGGLNTNVLRMVLN
                     QYKQWQSEGEEIDVCCIGGRGLGFMHRLGANVISEATGLGDRPTLQDLIGAVKVMLDG
                     YSEDQFDRVLIFYTRFINTMKQEPVMEQLLPLTGERIGIPEGSWDYIYEPEAKGVLDQ
                     VLTRYIESVVYQGLSENMASEQSARMVAMKSASDNAASLIDELTLVYNKSRQAAITNE
                     LSEIVAGAAAV"
     gene            6977..8401
                     /gene="atpD"
                     /locus_tag="VN496_09455"
     CDS             6977..8401
                     /gene="atpD"
                     /locus_tag="VN496_09455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXF17220.1"
                     /translation="MTQGTIVQCIGAVTDIQFPREAMPKVYDALVLEESNEASFAEKG
                     LTFEVEQQLGDGVVRCIALGSFDGLRRGMKVTSTDAPISVPVGAGTLGRIMDVLGRPI
                     DEGGPIKTETRRAIHQPAPKFAELSPSVDLLETGIKVIDLICPFAKGGKIGLFGGAGV
                     GKTVTMLELINNIATKHGGQSVFAGVGERTREGNDFYHEMIEAEVIKLDNLPESKVAM
                     VFGQMNEPPGNRLRVGLTGLTMAESFRDEGRDILLFIDNIYRFTLAGTEVSALLGRMP
                     SAVGYQPTLAEEMGRLQERITSTKTGSITSVQAIYVPADDLTDPSPATTFGHLDATVV
                     LSRDIASLGIYPAVDPLDSTSRQVDPNTIGEEHYSTTRQVQATLQRYKELRDIIAILG
                     MDELSPEDKLVVARARKIQRFLSQPFTVAQNFTGSPGKYVSLKDTIKGFKMIVNGECD
                     SLPEQAFYMVGAIEEAFEKAKTLQ"
     gene            8413..8832
                     /locus_tag="VN496_09460"
     CDS             8413..8832
                     /locus_tag="VN496_09460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXF17221.1"
                     /translation="MANTIHVDVVSAEQQIFSGEAEFVVLPGEAGELGIYPRHTPLIT
                     RIKPGAVRIQPAGGGPENLIFVAGGILEVQPSIVTVLADTAIRGHDLDEAKALEAKKR
                     AEDALRNAKDKQEIATVEAELSMLAAQLAAIRKMRRK"
     gene            complement(8994..9977)
                     /locus_tag="VN496_09465"
     CDS             complement(8994..9977)
                     /locus_tag="VN496_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXF17222.1"
                     /translation="MSAAREVLLKVDGLRKHFPAKAGLLSRGVKVLAVDGVSFSIHEG
                     ETLGLVGESGCGKSTTGKLILRLLDPTEGQVEWRGRRIEQLTRAEMRPVRRELQAVFQ
                     DPYASLNPRMRAADIVAEPLRNYESLGAGEVRTRTGNLFERVGLRADQLLKYPYEFSG
                     GQRQRIGIARALALRPSLIVCDEPVSALDVSVQAQVINLLMDLQAELNLSYLFVAHDL
                     AVVEHISHRVAVMYLGKIVEIAPKQTIFTQPQHPYTVALLEAVPVPDPTRRKQRRALG
                     GDVPSPIDPPSGCRFHTRCPHVEQRCRREEPPLQEVSPGQWVACHLRQPVA"
     gene            complement(9974..10957)
                     /locus_tag="VN496_09470"
     CDS             complement(9974..10957)
                     /locus_tag="VN496_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17223.1"
                     /translation="MTALLHVQDLKTRFFMRDGVIRAVDGVSFSMAAGETLAIVGESG
                     CGKSVTSLSILRLIASPPGRIVGGKILFEGRDLLALTEDEMRAIRGDAISMIFQEPMT
                     SLNPALTIGWQIAESLVLHRGLSKADAMTKAVEMLHKVRMPEPVRRARQYPHELSGGM
                     RQRVMIAMALACSPRLLLADEPTTALDVTIQAQILELMRELASETGAAIILITHDLGV
                     VAEMAQRVVVMYAGRKVEEAPVEQLFAHPRHPYTSGLLGSMPHLGHSLQDRSVKRLVE
                     IPGMVPSLREEHPGCLFAPRCPNAIARCLQEVPPLAAHGAGHSAACWNPMV"
     gene            complement(10957..11802)
                     /locus_tag="VN496_09475"
     CDS             complement(10957..11802)
                     /locus_tag="VN496_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF17224.1"
                     /translation="MSPTWQRFRDAFRRHPTAIAGAVVLVAMVLIALFAPWLATSDPQ
                     ALSPIQRLKHPSSGHWFGTDMLGRDVYSRVIFGSRISLTVGAAVAALSITLGLVIGLV
                     TGFVRWVDATVMRVMDGLMSIPSVLLAIALMALTKASVGNVIVAITLAEVPRVVRLVR
                     SLVLSLREQPYVEAAIATGTRLPLILLRHILPNTLAPLLVQGTYICASAMITEAILSF
                     IGAGTPPNVPSWGNIMAEARSMFQLASYLIFFPGAFLSVTVLAVNMLGDGMRDALDPR
                     LARRM"
     gene            complement(11837..12775)
                     /locus_tag="VN496_09480"
     CDS             complement(11837..12775)
                     /locus_tag="VN496_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF17225.1"
                     /translation="MVYIIRRLGATFIVMAVVALFVFSLLFLTPGDPAAVIAGDIATA
                     DDIKLIRTKLGLDEPFLVQFGSWVGRLLQGDLGTSIFTSLPVTKLIGQRVEPTVSLTL
                     LTLVIAVLIAVPVGVLAAAKAGSRIDRFVMGASVLAFSVPVFVLAYLLISLFSIELEW
                     LPVQGYRRLSEGVWPWLSHLILPSIALGTVYMALIARITRAAMLDVLSQDYIRTANAK
                     GLGSRAVLTRHALKNAGVPIITVIGIGVALLISGAIVTETVFAIPGIGRLTVDAILRR
                     DYPIIQGVILLFSGIYVLINLAVDLSYRLFDPRIQY"
     gene            complement(12807..14381)
                     /locus_tag="VN496_09485"
     CDS             complement(12807..14381)
                     /locus_tag="VN496_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17226.1"
                     /translation="MKFHCALLRAFMLALALAASSAAAQERTLKFIPQADLRILDPIT
                     TTAYITRNHGYMVYDTLFATDAKFKVQPQMVDKWDISKDKLTYTFTLRDGLKFHDGQP
                     VRSADCIASLERWSKRDALGQKLAQSTESWTVVNDKTFKLKLKKPFPLTLDALGKLSS
                     NVPFIMPERIAKTDPLQNISDATGSGPFKFVKDEWIPGSKVVYVKNTDYVPRKEAPSW
                     AAGGKVVKVDRVEWIYIPDSATAAAALNAGEADWWEQLPPDLIPLLRKNKDVRVENID
                     PLGSMGIMRFNFLQPPFNNQKMREALLYVVDQRDYVLGIAGDEKNGHTCYSFFTCGTP
                     LSSELGAEPLKGKRDFDKARKLIAEAGYKGERIVIISATDQPIVHSQSLITAELLRKL
                     GLNVELQASDWGTMITRRTSKEPVDKGGWSIFHTWWVGADIASPASMVMVNLGEKSWI
                     GWPADQKLEELRQAWFNTIDAAAGKKASDAVQVEAFQFVPYITTAQFILPTAYRSNLS
                     GLIVAPVTFLWNVEKK"
     gene            14709..15839
                     /locus_tag="VN496_09490"
     CDS             14709..15839
                     /locus_tag="VN496_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXF17227.1"
                     /translation="MSAQLNVIELTRQLVRMNTVNPPGQEEQCARHLGSLLEKNGFHT
                     RYHDLAPGRASLVATIGGRADKAPLCFTGHIDTVPLGAAKWNMDPFGADTESGKLYGR
                     GTSDMKCGVAAFVVASLNLAPSLERSPGLVLVITAGEETGCEGAFHLAHQTGALGRAG
                     AVVVGEPSSNYPFVGHRGAFWLNARTRGVTAHGSMPEKGDNAVFKAARAVAVLEKFRF
                     KNPPHPLMGQATLNVGTIRGGLNINSVPDEAVIGIDIRTIPNVTHAALKAELMRQLGN
                     EVELETLLDLESVFTEPTNPWIQEVFVVMRSILGRACEPRVATYFTDAAALNIAYNLP
                     PTVILGPGEAQMAHQTNEYCVIDRVTEAVAAYEEIARRWCAI"
     gene            15827..>16239
                     /locus_tag="VN496_09495"
     CDS             15827..>16239
                     /locus_tag="VN496_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXF17228.1"
                     /translation="MCNLIQEEVFSASLPTQRIASLLDSGSLTGAAQHGTLHVGSGVI
                     EGRQATVIATDRQAAGGSLGVTEAQALSAVLEESHAAGRVAILCLDSAGARLDEGLAA
                     LGAFRQLYRHLLDLRLAGFPMLALIGRNCFGGASML"
BASE COUNT         3620 a         4501 c         4792 g         3326 t
ORIGIN      
        1 gcgcggcaga cggtgtgatg atcgcaatga agggacttga tccgggagaa gaggtggcac
       61 gggtaccggc ttcgtggaaa gttatttcga ccttgcccct ggaaatacct cagttgggag
      121 cgtcccgtca tctcgttttt ctgcgaccag ccgctatggc cccgaattga ctcgaatggc
      181 acgtgtgtta gcggtcacta accagaaagg cggagtcgga aagactacga caacgatcaa
      241 cttggccgcc agcttggtaa gcgccgcgag acgggttcta ttggtagacc tggacccgca
      301 aggcaatgcc accatgggga gcggcattga caagcgcgcc ctcagaacga cggtatatca
      361 agtactgctt ggcgagactg cgatagcggc agtgagagtt cgcagccaga gtggaggatt
      421 tgatgttatt ccctcaaatc gcgaactcgc tggggcggag gttgaaatgg ttgacctggc
      481 agatcgcgaa accaggctaa agaatgcctt gcgcgaaatc caggatgaat acgacttcgt
      541 gctgattgat tgtccgccgg cgctaaacct cttgacggtg aatggccttt gcgcggctaa
      601 cgcagtaatg attccgatgc agtgtgaata ctatgcgctc gaaggtttga gcgacctggt
      661 tcaaaccatc aagaaagtgc gagttagctt gaatcccacc ctggagatcg aaggtttgct
      721 gaggacgatg tacgacccac gcaatatgtt ggcgcaacag gtatcggacc aactgcagca
      781 gcactttggc gacaaagttt accgaaccat cattccgcga aacatccgcc ttgctgaagc
      841 tcccagccac ggccttcccg cgcttagttt cgaccgccag tcgaaagggg cattggccta
      901 tctggcgctg gctggcgaaa tgctcggcaa agttgcttag gacaaaaatg gccaaattaa
      961 agggactggg acgtgggctg gacgcattgc tgcacggaaa cgaagagcag acccacaatg
     1021 cgcaggctga attgccggtt gagttattaa cgccgggacg atttcaaccc cgtacgcgga
     1081 tggatccgca atccattgcc gacctggccg cttcgatcaa gtcccagggc ctgattcagc
     1141 caatcctggt acgcacgagt gacaacggca aatatgaaat catcgccggc gaacgccgct
     1201 ggcgggcttc gcagctggcc ggacttacgc aggtgccagt aatcgttcgc gaggttccgg
     1261 acaaatcggc cttggctatg gcgctcatcg agaatatcca acgcgaagac ttgaatccac
     1321 tggaagaggc aaccggcatc cagcgcctga tagacgaatt cgagatgacc catgatgtcg
     1381 cggcagaagc ggtagggcgt tcgcgtagcg ccgttacaaa tttactgcgg ctgcttaatt
     1441 tgtcgaaagc ggtccaggat ttgttgatgg agggcaagat cgagatgggc catgcacgcg
     1501 ccctgctggc catcaatggc tcgcgtcaag ccgagctggc ccgccagatc gtcagcgata
     1561 atctctcagt gcgtgaaact gagcagctgg taggggcggc ggcgagaact acgcgcaaag
     1621 ctgtgcgcag acgtccgaga aaagaccgcg acctccaggc actcgaagaa gaattgtccg
     1681 agaagctcgg taccacggtg gcttttaaaa cgttgcgagg cggccggggt aaattgacca
     1741 tcgattatgc aagcctcgat cagctggaca acgtactaaa attattgcgc cactgatgcg
     1801 caatcgatat cagcgtacat aacgttgacc cgaaattgcg gaacccttta caatcgaccg
     1861 tttgctcccg cgtagttgtc ctgcgatgat gctgcggatt cagagcaagc cagtgcgtac
     1921 agttcttttg tggcagattg cggtaacaac attcctggca attattgccg gcttgctgac
     1981 cggcattcac gcaatggcgt ctgcggccct cggcggacta gtcagcgtta cagccggact
     2041 ggcgttcgca ctggtcatgc aatctggtgg ggcgcaatct gccgatggca ctgttatcgc
     2101 agcattgagg gctgaagcaa caaagatcgg tgttgcggtg attctgctgt ggctggtact
     2161 ggcactttat aaggatgtgg tgccaatcgt tttcatcggc tcgtttattg tttcgattct
     2221 ggtgttttcg atggcgtttt tcgtacgaga cgtatagatt taatgcataa cgatggcaga
     2281 agctagcgaa ctgaccccaa acgaatatat ttcccaccat ttgacgaaca tgacatcgtc
     2341 gatgggtcag ggtgatttct gggtgctgca cgtcgatacc ctggtaatgt cggtcctgct
     2401 tggaattgtc agtctcggct ttctctggtg ggctgtacgc ggcgcgactg ccggcatacc
     2461 cggaaagcgt caggcctttg tcgagctggc agtggaattt gtggatgacc aggtcaaagg
     2521 aatattccac ggcgatcggc acaagtttat ttcgccagcg gcattgaccg tcttcgtttg
     2581 ggtgctgctg atgaacgcca tggatttcct tccggtggac atcatggcct ggttctatga
     2641 agacgtgctg catcttcaca attggcgcgg cgtgcccacc gcggatgtca acaccacctt
     2701 cgcgcttgcc ctgtcagtct ggctgctgat gatctatttc agcatcgcgg tcaaggggct
     2761 agggggatgg attcatgaat tgttcgggac gccattcggc aatcatgtaa tcctgtggcc
     2821 cgcaaacctt ctgtttaacc tcgttgaata tatctccaag ccgctgtcgc attcattgcg
     2881 gctgtacggc aacatgtacg cgggggaaat cattttcctg ctgctgggac tttgggcggc
     2941 tactggactg gtcggcgccg tggcgggtgt gttcctcggc gccggctggg cgatctttca
     3001 cattttaatc gtcgttctgc aggcattcat tttcatgatg ctgactgtcg tgtatctctc
     3061 gatggcgcac gaaagtcact aattaagcca tccactttta ctgagaagaa ggaaggaaaa
     3121 ccgaaatgga aaaaatgcaa tttctggcac tgatccaggc gtacaccggt gtcggaatcg
     3181 gcctcatgat tggactgggt gccgcgggcg catgtattgg cgtgggcatt atgtgcagcc
     3241 ggtttctgga gggcgcggct cggcagccag agatgatccc ggcgttgcag gccaaggttt
     3301 tcctgttgct cggcttgatc gatgcttcat tcatcatcgg agtgggtctg gcgatgttgt
     3361 tcgcattcgg caatccgctg ctcgctgtca ttcgctaagc tccttcggat aatagtttag
     3421 gagacaaccg tgaacatcgg tttaaccatg ctctcccagg cgatcgcttt tgcgatcttt
     3481 atctggttca caatcaagtt catctggcct ccgctcatgc acgccataga gcagcgtcag
     3541 aaacagatcg ctgaaggtct ggccgccgcc gagcgcggca aacaagatct ggagctcgct
     3601 gccaagcggg cagccgagga actgcaaaac gcacgtgtgc aagcaagcga aattatctcg
     3661 caggcagaaa aacgaggcgc cgcggtcata gaggaagcca aaagcgcagc caaggcagaa
     3721 gcggagcgta ttgtcgttgg cgccaaggca gagattgagc aggaagtttt ccgcgccagg
     3781 gagtctttgc gtacacaagt tgcggttctt gcggttacgg gcgcagagaa aatcctgaag
     3841 cgcgaagttg atgccaaggc acacgcgcag atgttgtcgg gcctggaagc ggaactcagg
     3901 taagaagatg gctgaactcg ccacgattgc gcggccttat gccgaagcag tattccgtct
     3961 cgcaaagcag accaatgctc tgggcgcgtg gtcggatgcg ctaaatttaa tcgccactgt
     4021 ataccgggat ccccagatgc aagccgcggt ggcgaatccg aaggtcacgc cgtctgaaat
     4081 cgagcgactt atgctggcca tatgtggtga gcgtatcgac ggggtggcgc gaaacctgat
     4141 ccagttgctg gtgcataacc gcaggctatc tctgctcacc gagattcgcg ctttgttcga
     4201 gcagctaaaa ctggaagatg agggcaagct ggatgccaaa atcagttctg ccttccccat
     4261 ggaagacacg cagcgaaatc aggttgtgaa cctgctgtct tcacggttca aacgcaagat
     4321 caatgccact gtgattgtgg attccaatct gatcggaggc atcaaagtcg aggtcggtga
     4381 caagatttgg gatgcttcag tccggggccg gttgcagaac atggccgcgg cacttacgaa
     4441 ataggagttt gagtcatgca attgaatcca tcagaaataa gcgaacttat caggagcaag
     4501 atccagaatc ttgcctctac ttccgaggta cgtacacaag gcacggtggt ttccgtaacc
     4561 gacggaattt gccgcataca cggtctggcc gacgtcatgc aaggagaaat gctggaattt
     4621 cccgacaata cgttcggtct tgcattaaac ctcgagcgcg actcggtcgg cgcagtggtc
     4681 ctgggaaact acgagcacat ttccgaaggc gatccagtca agagtaccgg tcgcattctg
     4741 gaggtacccg tcggaccgga actgcttggc cgcgtggtgg acgcgttggg ccaacccatc
     4801 gatggcaagg gaccgatcgc agcgaaagcg ttcgacgtca tcgaaaaagt ggcgccgggc
     4861 gtgatttggc gtaaatcagt atcgcagccc gtgcagaccg gccttaaggc aatcgacgcc
     4921 atggttccaa tcggccgcgg ccaacgtgag ttgattatcg gcgatcgcca gaccggcaag
     4981 accgctgtcg ctgtggacac catcatcaat caaaagggca aagacctcct gtgcgtgtac
     5041 gttgccatcg gccagaaagc atctaccgta gcaaacgtcg tacgcaaact ggaagagcac
     5101 ggtgcgatgg cctataccat cgttgtggcc gctactgcgt ccgaatccgc agcattgcaa
     5161 tacatagcgc cttattcggg ctgcaccatg ggcgagtact tccgtgatcg tggtcaggac
     5221 gcactgatta tttatgatga cctgaccaag caggcttggg cctatcgcca aatatcgttg
     5281 ctgttgcgtc ggccgccggg ccgggaagcg tatcccggcg atgtttttta tttgcactca
     5341 cgcctgttgg aaagagctgc gcgcgtaagc gaagcttggg tagagcgctt taccaacgga
     5401 gccgtcaaag gcaagacagg ttctctgacc gcgttgccca tcatagagac ccaggccggc
     5461 gacgtatccg cgttcgttcc aaccaacgtg atttcaatta ccgacggcca gatttttctc
     5521 gagacggatt tgttcaatgc gggcatccgg ccagcaatca acgccggggt atcggtttcg
     5581 cgcgtcggtg gcgctgcgca gaccaaaatc atgaaacgca aggataccgg cggcggcgcg
     5641 cgcctggcat tggctcagta ccgcgaactg gcggcgttcg cgcaatttgc ctcggacctg
     5701 gacgaatcaa ctcgtaagca gctcgaccgg ggccgcatgg ttacggaact gatgaagcaa
     5761 ctgcagtacc agccgctcca ggtctgggaa atggcgctga cgttgttcgc cgccaacaat
     5821 ggccacttcg atgacatcga tgtcaagaag gcactgtcgg ccgaaaattc tcttcgcgat
     5881 ttcataaaga caaaatatgc ggcactcgct cttcgcatgg acaaggactt atccggagac
     5941 gatgagaaag cactcaacga ggcaatcaag gactggaaga agaacgccac ctattgaaac
     6001 cgcggcggca gctgttcgct ttttctgaat taaatcgtac tgtggactga atcgatggcg
     6061 ggaacaaaag agatacgaaa caagatcaag agtgtgcaga gcactcgcaa gatcaccaag
     6121 gccatggaat tggtcgccgc gtccaagatg cgccgtgcac aagagcgcat gcgtgctgcg
     6181 cgcccttacg cggagaaaat ccgcaacgtg gcggcacaca tatcgtacgc gaaccccgag
     6241 taccggcatc cgtttctgat tgcgcgcgat accgtgaagc ggatcggcat tattgtcatc
     6301 accaccgaca aaggactgtg cggtggattg aatacaaatg tgctgcgcat ggtgctcaac
     6361 cagtacaagc aatggcagag cgagggagaa gagatcgacg tttgctgtat cggtgggaga
     6421 ggccttgggt tcatgcatcg cctcggtgca aatgtcatat cggaagcaac cggcctgggc
     6481 gatcgtccga cgctgcaaga tctaattgga gcagtgaaag tcatgctgga tggctacagc
     6541 gaagatcagt ttgaccgcgt gctcattttc tacacccgct tcatcaacac catgaaacag
     6601 gagccggtga tggagcagtt gctacccctg accggagaac gcatcggcat accggagggg
     6661 agctgggatt atatctatga gccggaagcc aaaggtgtgc tggatcaggt gctgactcgt
     6721 tacattgagt cggtggtgta tcaagggctt tcggaaaaca tggcctccga gcaatcggcg
     6781 cgtatggtcg cgatgaagtc cgcatcggac aatgccgcat cgttgatcga tgagctgact
     6841 ttggtttaca acaagagccg gcaggctgcg attaccaacg aactgtcaga gattgtcgcc
     6901 ggcgcagcgg ccgtatgagg ccgttctagc tcaggattta ttcaatgaat ttgtcttacg
     6961 cttttaggaa aacatcatga cccaaggaac catcgttcaa tgcatcggcg cagtgaccga
     7021 catccaattt ccgcgcgaag ccatgccgaa agtctatgac gcattggtac tcgaagagtc
     7081 gaacgaggca tcgtttgccg agaaggggct taccttcgaa gtcgaacagc agcttggcga
     7141 cggcgtggtc cgctgcatcg cgctggggtc ttttgacggc ttgcgccgcg gcatgaaagt
     7201 aacttccacc gacgcgccga tctcggtgcc ggtcggcgca ggcacgctgg ggcgcatcat
     7261 ggatgtgttg ggacgcccga tcgacgaggg cggcccgatc aaaacggaga cacgccgcgc
     7321 gattcatcag ccagcgccca aattcgcgga actgtcgcct tcggtcgatc tgctcgaaac
     7381 gggcatcaaa gtaatcgacc tcatctgccc gttcgcgaag ggaggcaaga tcggtctctt
     7441 cggtggtgcc ggtgtgggca agaccgttac catgctcgag ctgatcaata atatcgccac
     7501 caagcacggc ggccagtccg ttttcgcagg cgtgggcgaa aggacgcgcg agggcaacga
     7561 cttttatcac gaaatgatcg aggcggaggt catcaagctc gataatctcc cggaatccaa
     7621 ggtcgcgatg gtatttggcc agatgaacga accgccgggc aaccgtttgc gcgtcggtct
     7681 caccggcctc acgatggcag agtcgttccg ggacgaaggg cgggatatcc tgctcttcat
     7741 cgataacatt tatcgcttta cgctagcggg aacagaagtc agcgcactgc tcggccgaat
     7801 gccttcggca gtaggttatc aaccgacgct cgccgaagag atgggccggc tgcaagagcg
     7861 catcacttcg acgaaaaccg gctcaatcac ttcagtgcag gcgatttacg tgccggccga
     7921 tgacctgact gatccgagcc ccgccacgac gttcggccac ctggacgcca ccgtcgtgct
     7981 gtcccgggac atcgcgtcac tcggcatcta tcccgccgtc gatcctctgg actcgacctc
     8041 gcgccaagtg gacccgaata ccattggcga ggagcactac agcaccacgc ggcaagtgca
     8101 agcaaccttg cagcgctaca aggaactgcg cgacatcatc gccatcctgg gcatggacga
     8161 actctcgccc gaagacaaac tggtggtcgc gcgggcgcgc aagatccagc gcttcctgtc
     8221 gcagccgttc actgtggcgc agaacttcac cggttcaccg ggcaagtacg tctcgctcaa
     8281 ggacacgatc aaaggtttca aaatgatcgt caatggcgag tgcgatagcc tgcccgagca
     8341 agccttctac atggtcgggg caattgaaga agcattcgag aaggcaaaga cactgcagta
     8401 ggaaagcttt caatggcgaa tacgatccat gtagacgtgg tcagtgccga gcaacagata
     8461 ttttctggcg aggcggaatt tgtggtgttg cctggcgaag cgggcgaact gggaatttac
     8521 ccgcggcata cgccattgat cacgcgcatc aagccgggcg cggtgcgcat tcagcctgcc
     8581 ggcggcggcc cggaaaatct gatatttgta gccgggggca ttctggaggt gcagccctcg
     8641 atcgtgactg tgttagcaga cacggccatt cgcggacacg atctcgacga ggccaaagcc
     8701 ctcgaagcga agaagcgggc tgaggatgca ctgcgcaatg ccaaagacaa gcaggaaatt
     8761 gcgacggtag aagctgagct atcgatgttg gcggcgcaac tggcggcgat tcgtaaaatg
     8821 cgccgcaaat aagaggacct tattccccaa ggcaaagagc ggcttcggcc gctttttttg
     8881 tttctccatc tttgtcgcga acttcccgac ccaaggcggc cgcatgtcgc gaaagcgaga
     8941 gcaaaccaca agggaaatgt agttaacacg tcactggctt cgcgctgata atttcaagcg
     9001 accggctgtc gcaaatggca cgctacccac tggcccgggg aaacctcttg tagcgggggc
     9061 tcttcgcgcc tgcatcgctg ctcgacatgg ggacagcgcg tatggaaccg gcaacctgat
     9121 ggcggatcga tcgggctcgg cacgtcgcct ccaagtgcgc ggcgctgctt gcgacgggtc
     9181 gggtcaggca cgggcacagc ctcgagcagc gctacggtat aggggtgctg cggctgtgtg
     9241 aagatggtct gcttgggcgc tatctcgacg atcttcccga ggtacatcac cgcaacgcga
     9301 tggctgatgt gctcgaccac ggcgagatcg tgcgcgacga agaggtaaga cagatttagc
     9361 tcggcctgca gatccattag cagattgatt acctgagctt gcactgaaac atccagcgcc
     9421 gacacgggct catcgcacac gatcagactc ggccgcagtg ccaacgcgcg tgcgatgccg
     9481 atacgctgac gttgaccccc ggaaaactcg tacggatatt ttaagagctg atcggctcgc
     9541 agtccgacac gctcaaatag attcccggtg cgcgtacgca cctcgccagc ccccagagat
     9601 tcgtagtttc ggagcggctc tgcgacgatg tccgccgcgc gcatgcgcgg gttcagggag
     9661 gcgtagggat cttggaacac cgcttgcagc tcgcgccgca ccggacgcat ttctgcccgg
     9721 gtcagctgtt cgatgcgcct gccgcgccac tcaacctgac cttcggtggg atcgagcaac
     9781 cgcagaatca gtttgcccgt tgtcgactta ccgcagccgg actcaccgac cagcccgagc
     9841 gtctcgcctt catgaatcga gaagctcact ccgtcgacgg cgaggacttt aacgccgcgc
     9901 gaaagcagac cggccttggc cgggaaatgt ttgcgcaggc catctacttt gagcaacacc
     9961 tcgcgtgccg cgctcatacc atggggttcc aacaggcggc agagtgccct gcgccgtgcg
    10021 cggctagcgg aggcacctcc tgcaagcagc gtgcaatcgc gttcggacaa cgtggagcaa
    10081 acaaacaccc cgggtgctct tcccggagag acggcaccat tcccggaatc tctaccagcc
    10141 gcttgactga acggtcttgt aacgaatggc cgaggtgcgg cattgatcca agcagcccgc
    10201 tcgtgtaagg gtggcgagga tgcgcgaaca gttgttcgac cggcgcctcc tcgaccttac
    10261 gaccggcata catcaccacg actcgctgtg ccatttccgc taccacgcca aggtcgtgcg
    10321 tgatcagaat aatcgccgcg ccggtttcgc ttgcgagctc gcgcatgagc tccagaatct
    10381 gcgcctggat cgtcacgtcg agcgcggtgg taggctcgtc ggccagcaac aatcgcgggc
    10441 tgcaagcgag cgccattgcg atcatgactc gttgccgcat tccgcccgac agctcatgcg
    10501 gatactggcg cgcgcggcgc actggctctg gcatgcgtac cttgtgcagc atctctacgg
    10561 ctttcgtcat cgcatcggcc ttcgagagac cgcggtgcaa gaccaggctt tcggcaattt
    10621 gccagccgat agtcagcgca gggttcagcg aagtcatcgg ctcctggaaa atcatcgaaa
    10681 ttgcgtcgcc gcgtatcgcg cgcatctcgt cttcagttag cgcgagcaga tcgcgacctt
    10741 cgaacaaaat tttgcccccc acaatgcggc cgggggggga ggcgatcagc cgcaggatcg
    10801 agagcgatgt cacgctcttg ccgcagcccg actcgcccac gatggcaagt gtctcgcctg
    10861 ccgccatcga gaaagagact ccgtctaccg cgcgtatgac gccatcgcgc atgaaaaagc
    10921 gggttttgag gtcttgaaca tgcaaaagcg ctgtcatcac attcgtcgag ccaagcgtgg
    10981 atcgagtgca tcgcgcatgc cgtcacccag catattcact gcgagcaccg tgacagacaa
    11041 aaacgcgccg gggaaaaaga ttagataact ggcgagctgg aacatgctcc tcgcctccgc
    11101 catgatattg ccccagctcg ggacgtttgg tggtgtaccg gcgccgatga aagacagaat
    11161 cgcctccgta atcattgctg atgcgcagat gtaagtgccc tgcacgagca gcggcgccaa
    11221 ggtattgggc agaatgtgtc gaagcaggat gagaggcagt ctggtgcccg tggcgatcgc
    11281 agcttccacg tagggctgct cgcgcagact caacactagg ctgcgcacca ggcgcaccac
    11341 gcgcggcacc tccgccagcg tgattgcaac aatgacgttc ccgacactgg ctttggtcag
    11401 tgccatgagt gcgatagcga gaagcaccga gggaatcgac atcagcccgt ccataacgcg
    11461 catgacagtt gcatccaccc agcgcacgaa tccggtgaca aggccaatta cgaggccgag
    11521 agtgatggat agagcagcaa ccgcggcgcc gaccgtcagc gagatgcggg aaccgaagat
    11581 tacacggctg tacacgtcgc ggcccagcat gtcggtgcca aaccagtgcc cgctcgacgg
    11641 gtgcttaagt cgctggatcg gtgagagcgc ctgcggatcg gaggtggcga gccaaggtgc
    11701 aaacagcgca atcagaacca tcgcaaccag cacgacggcg ccagcaatcg cggtcgggtg
    11761 tcggcggaag gcatcgcgga aacgttgcca cgtcggcgac atggagcgcg gtgccgcaat
    11821 tgctgcagta ttcatgtcag tactggatac gcgggtcgaa caacctgtag gagagatcaa
    11881 cagccagatt gatgagtacg tagattcctg agaagaggag gatcacaccc tgaatgatcg
    11941 ggtagtcacg ccggaggatc gcatctactg taagccgtcc gatgcccggg atagcgaaga
    12001 ccgtctcggt gacaatggcg ccgctgatga gcagtgcgac gccgatgccg attaccgtga
    12061 tgattggcac gcccgcattc ttcagcgcgt ggcgcgtcag caccgctctc gatccgagcc
    12121 ccttcgcgtt ggccgtgcga atgtagtcct gggacagcac gtccagcatt gccgcgcgtg
    12181 tgatgcgggc gatcagcgcc atgtagaccg tgccaagtgc gatgcttggc agtatcaaat
    12241 gcgacagcca cggccacacg ccctcgctga gccgccggta gccctgcaca ggcagccatt
    12301 ccagctcgat ggaaaagagg ctgatcagca gataagccag cacgaacact ggcacggaga
    12361 atgccagtac cgaggcgccc atgacaaacc gatcgatccg gctaccggct ttcgccgccg
    12421 ccaatacacc gaccggcacc gcgatcaata ccgcaatcac cagtgtaagc agcgttaacg
    12481 ataccgtcgg ctcaacgcgc tgcccaatca gcttcgtaac cggaaggctg gtaaagattg
    12541 atgtgccgag atcgccctgc agcagccgac cgacccaact cccgaactgc accaggaacg
    12601 gctcatccag accgagttta gtgcggatga gcttgatgtc gtcggcggtc gcgatgtctc
    12661 cagcaatgac cgcagccgga tctccgggcg tcaggaacaa cagcgagaac acgaacagtg
    12721 cgaccaccgc catgacaatg aacgtcgctc caagacggcg aataatgtag acaatcatgt
    12781 catgtgaagt gaagtaccgc ggcggcttac ttcttttcga cgttccagag aaacgtgaca
    12841 ggagcaacaa tcagcccgga aagattagag cgatatgcgg tcggcagaat gaattgcgcg
    12901 gtggtgatat atgggacgaa ctggaatgct tcgacctgaa ccgcatcgga agcctttttg
    12961 cccgcagccg cgtcgatcgt gttgaaccag gcttgacgca attcttccag tttttgatcg
    13021 gctggccagc cgatccagga tttttcaccg agattcacca tcaccatgga ggccggagag
    13081 gcgatatctg ccccgaccca ccaagtgtga aagattgacc agccgccctt gtccaccggc
    13141 tccttcgagg tccggcgcgt aatcatcgtg ccccaatcgc tggcctgcag ctcgacgttg
    13201 aggcccagct tgcgcaacag ctccgccgtg atgagcgatt gcgaatgcac gatcggctgg
    13261 tcggtggccg agataatgac gattctctcg cctttgtagc cggcctccgc gatgagcttt
    13321 ctggctttgt cgaagtcgcg tttgcctttt agcggttcgg cacccagctc cgaggacaac
    13381 ggcgtgccgc acgtgaaaaa cgaatagcag gtatgaccgt ttttctcatc gccagcaatg
    13441 ccgagcacgt aatccctctg gtcgacgacg tagagcagtg cttcccgcat cttttggttg
    13501 ttgaacggag gctgcaggaa attgaagcgc atgatgccca ttgacccaag cggatctata
    13561 ttctcgaccc ggacgtcctt gttcttacgc agcagcggga tcagatcagg cggcagctgc
    13621 tcccaccagt cggcctcgcc cgcgttcaag gccgcagccg cggtagctga atcgggaatg
    13681 taaatccact cgacccggtc tactttgaca accttacccc ccgcagccca cgatggcgcc
    13741 tccttgcgcg gcacgtaatc cgtgttcttg acatagacca ccttgctgcc gggtatccac
    13801 tcatccttca cgaacttgaa cgggccggaa ccggtcgcat ccgagatgtt ctgcagcggg
    13861 tctgtcttgg cgatgcgttc cggcataatg aacggaacgt tcgacgatag tttgccgagc
    13921 gcatccaggg tgagcggaaa cggtttcttg agcttgagct taaaggtctt gtcattcacc
    13981 accgtccacg actcggttga ttgcgccaac ttttgaccga gcgcatcgcg cttggaccag
    14041 cgctccaagg acgcaatgca gtcagccgag cgcactggct ggccgtcgtg gaacttgagc
    14101 ccatcccgca gcgtgaacgt gtaggtcagc ttgtctttcg agatatccca tttgtcgacc
    14161 atctgcggct gcaccttgaa cttggcgtcg gtcgcaaata gcgtgtcgta caccatatag
    14221 ccgtggttgc gcgtgatgta ggccgtggtc gtaatgggat cgagaatgcg cagatcagcc
    14281 tgcgggataa atttcagagt cctttcctgc gcggccgctg aactcgcagc tagcgcaagg
    14341 gccagcatga atgcgcgaag caaagcgcaa tggaacttca tgatcttctc ccgtacgagg
    14401 attcttttgc caatgtgccg gcgcccagag ccgactttat cgctgcaatc aatgaaagta
    14461 taggccgcca ccacgcgcgg ctgccagccg aactatgaag ttcgagaatc ggtggcaatt
    14521 gcaaattaag atctgcatac gcatgacctt cacgctgccg ggttgcgcgc gcagttcatg
    14581 ttaccggata gcccttgcca gcggcgttac gtaccgcatg gccttggctt tttgcggtta
    14641 acatttgtgc ggacagcgcg gatgcgtttt aatgcgggcc aggtgtttcg ggaggtattg
    14701 gaagcggtat gtctgcgcaa cttaacgtaa tcgaacttac caggcagctc gtgcgcatga
    14761 ataccgtcaa tccgccgggc caggaagaac aatgcgcgcg ccatctaggt tcattgttgg
    14821 aaaaaaacgg cttccacacc aggtatcacg acttggcacc ggggcgggct agccttgtgg
    14881 cgaccattgg cggccgcgca gacaaggccc ccctttgctt caccggacat atcgataccg
    14941 ttccgcttgg cgctgcaaaa tggaatatgg atccgttcgg cgccgatacg gaaagcggca
    15001 aactgtacgg acgcggtacc agcgacatga aatgtggcgt ggccgctttt gtcgttgcat
    15061 cgctgaatct ggcgcccagc ctggaacgtt cacccggtct ggtgttggtc atcacggcgg
    15121 gcgaagaaac cggctgtgaa ggcgcctttc atctggctca tcagacgggg gcattgggtc
    15181 gggctggagc tgttgtcgtt ggcgagccat cgtccaacta tccgttcgtg ggtcatcgcg
    15241 gtgccttctg gttgaatgca cgcacgcgcg gcgttacggc gcatggttcg atgccggaaa
    15301 aaggcgacaa tgcggtgttc aaagcagccc gagccgtagc ggtactggag aaattccgtt
    15361 tcaaaaatcc gccccacccc ttgatgggtc aggcaacgct gaacgttggc actattcggg
    15421 gcgggttgaa tatcaattcc gtgcccgacg aggctgtgat cgggattgat attcgaacca
    15481 taccgaacgt cacgcatgcc gcattgaaag ctgaattgat gcggcagctg ggcaatgagg
    15541 tggaactgga aacgctgctc gacctggaaa gtgtgttcac ggagccgacc aatccatgga
    15601 tccaggaagt gtttgttgta atgcgatcca ttcttggcag ggcttgcgag cctcgcgtag
    15661 cgacctactt caccgacgcg gccgcgctca atatcgcgta caaccttcca cccaccgtca
    15721 ttcttgggcc gggcgaagcg cagatggctc atcagaccaa cgaatattgc gttattgatc
    15781 gggtcacgga agcagttgcg gcctacgaag aaattgcgcg acgctggtgt gcaatttgat
    15841 acaggaagaa gttttttccg caagtcttcc aactcaacgc atcgcgtcgt tgctcgatag
    15901 tggatcgttg acaggggccg cacaacacgg aacgctgcat gtcggcagcg gcgtcattga
    15961 agggcgtcag gcgactgtta ttgcaaccga taggcaagcg gccggcggat ccctgggagt
    16021 caccgaggca caggctctct ccgcagtttt ggaggagagc catgcagcgg gacgggtcgc
    16081 gatcttgtgc ctggactccg ctggcgcaag actggacgaa gggctggccg cgctcggtgc
    16141 attccgccaa ctctacaggc atctgcttga cctgcgcctc gccggcttcc ccatgctcgc
    16201 gttgataggg cggaattgtt ttggcggagc gagcatgct
//
LOCUS       DATMZQ010000291         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4037816, whole genome shotgun sequence.
ACCESSION   DATMZQ010000291 DATMZQ010000000
VERSION     DATMZQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4037816"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1708
                     /locus_tag="VN496_09500"
     CDS             11..1708
                     /locus_tag="VN496_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF17229.1"
                     /translation="MYESASSAPIVDPPGKSENVSKARPQTGLLKASALQNAILTSAN
                     FSIIATDEKGIIQLFNIGAERMLGYTAAEVVNKISPSDIHDPQEVMARATALSLELGT
                     SIAPGFEALAFKASREIEDSYELTYIRKDGSRFPAVVSITALRDDDQRIIGYLLIGTD
                     NSVRKRVESELNEAMAAAEKANRAKTDFLSSMSHELRTPLNAILGFAQVMESGVPPPT
                     ASQKRSLEQILKAGWYLLELINEILDLALIESGKATLSREPVSLAEVMLECRAMIEPQ
                     AHSREIRMTFPTFESACFVNADRTRIKQVMINLLFNAIKYNKPGGSVSVEYSLSQDSV
                     RINVRDTGVGLAPEQLSQLFQPFNRLGKERSTIEGTGIGLVVTKRLVELMGGTIGADS
                     TVGVGSVFWIEMAVTVAPQLVRNGAEPVSLERPLPMTGAPMRTLLYVEDNPANLELVE
                     QLIARRPELRLLSAADGNQGIEYARTYRPEVILMDINLPGISGLEVMKILRADVQTAH
                     IPIIALSANAVPRDIENALEAGFFNYLTKPIKVNQFMDALDVALIFAQSVSGQAAPA"
     gene            1708..2529
                     /locus_tag="VN496_09505"
     CDS             1708..2529
                     /locus_tag="VN496_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF17230.1"
                     /translation="MIAESEILNASILIVDDQEVNVALLEQMLQEAGYRCIESTQNAH
                     EVCALHRRNRYDLILLDLQMPGMDGFQVMESLKTNENDDYLPVLVITAQPGHKLRALQ
                     IGARDFVSKPFDLVEVKTRIRNMLEVRLLYKKLEHYNNDLERTVLERTAELRESEARY
                     RSLTELASDWYWEQDESGNFTKVSGPVLEMLGIRVEALVDDANRDDVAGWDEEQRKKL
                     QAIIAARQPFLDFVFSRANKDGSIQRFQVSGEPMFNRSCGFIGYRGIGVELTQKM"
     gene            2554..3017
                     /locus_tag="VN496_09510"
                     /pseudo
     CDS             2554..3017
                     /locus_tag="VN496_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465817.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
BASE COUNT          695 a          845 c          875 g          602 t
ORIGIN      
        1 gaaaatcgcc atgtatgaat cagccagcag cgcgccaata gtcgacccgc ccggcaaatc
       61 tgaaaacgtc tcaaaggcca gaccccagac tggattgctt aaagcgagcg cattgcaaaa
      121 tgcgattctg acaagcgcca atttctcgat tatcgccacc gacgagaaag gcatcattca
      181 actgttcaac atcggcgccg agcgcatgtt gggctatacg gccgcggaag tcgtcaacaa
      241 gatcagcccg agcgacatac acgatccgca ggaagtcatg gcgcgcgcca cggcattgag
      301 tctggaactc ggaacttcca ttgccccggg attcgaggcg ttggcattca aggcttcgcg
      361 agaaatcgag gacagctatg aactgactta tatccgcaag gatggcagcc gctttccggc
      421 tgtcgtatcg atcacggcgc tgcgggacga cgaccagcgc atcatcggat acctgctgat
      481 cggaactgac aattcggtac gcaagcgcgt cgagtcggaa ctcaacgaag ccatggcggc
      541 cgccgagaaa gcgaatcgcg caaaaactga ctttctgtcc agcatgagcc atgagttgcg
      601 cacgccgctt aatgccattc ttggttttgc gcaggtcatg gaatccggcg tcccgccccc
      661 aactgcgtcg caaaaacgca gcctcgagca aattctcaag gccggctggt atctattgga
      721 gttgatcaac gaaatactgg acctggcgct catcgaatcc ggcaaggcca ctttatcccg
      781 ggagccggtt tcactggccg aggtcatgct ggaatgccgt gcgatgatcg agccgcaggc
      841 gcactctcgc gaaatccgca tgacgtttcc aacatttgaa agcgcctgct tcgtcaatgc
      901 cgaccgcaca cgcatcaagc aggtcatgat caatcttctt ttcaacgcca tcaagtacaa
      961 caagccggga ggctcggttt cggtggagta cagtttgtcc caggattccg ttcgtattaa
     1021 cgtgcgcgac acgggcgtgg gccttgcgcc ggaacaactg tctcagctat ttcagccttt
     1081 caaccgatta ggaaaggaac gaagcacaat cgaaggcacg ggcatcggcc tggtggtgac
     1141 caagcggctg gtggagctca tgggcggaac gatcggtgcg gatagtactg tcggcgtggg
     1201 gagtgtgttc tggatcgaaa tggcggtgac ggtcgctccc cagcttgtgc ggaacggggc
     1261 cgaacctgtg tcattggaac ggccgttacc catgacaggg gcgccaatgc gcacgctgct
     1321 atatgtagaa gacaatcccg ccaatctgga actggtggag cagctgattg cccgccgacc
     1381 cgaattgcgc cttctgagcg cggccgacgg gaatcaggga atagaatacg cgcgcactta
     1441 ccggccggaa gtcatcctga tggatattaa tctgcccggc atcagcggcc tggaagtcat
     1501 gaagatcctg cgggcagacg tgcagactgc gcatattcca atcattgccc tgagcgcgaa
     1561 tgcggtgccg cgcgacatcg aaaatgcgct ggaggccggc ttcttcaact atctgaccaa
     1621 accgatcaag gtcaaccagt tcatggatgc cttggatgtt gctcttatat ttgcacaatc
     1681 ggtatcgggg caggcggcac cagcatgatg attgcagagt ctgaaattct taacgccagc
     1741 atcctgatcg tcgacgatca ggaggtcaac gtggccttgc tcgaacagat gctgcaggaa
     1801 gctggctacc gctgcattga gtccacgcaa aatgcccacg aagtttgcgc gctacatcgc
     1861 aggaaccgtt acgacctcat tcttctcgac ctgcagatgc ctggcatgga tggattccag
     1921 gtcatggaga gcctcaagac aaatgaaaac gatgactatc ttcctgtcct ggtcatcacc
     1981 gcccagccgg gacacaagct ccgggcgttg cagatcggag cgcgagactt cgtcagcaaa
     2041 cccttcgacc tcgttgaagt aaaaacccgc atccgcaaca tgctggaggt caggctgctc
     2101 tacaaaaagc tcgagcacta caacaacgac ctggagcgca ccgtgctgga gcgcacggcg
     2161 gagctgcgcg agagcgaagc ccgctatcgc agcctgacag agcttgcctc cgactggtat
     2221 tgggagcagg acgagagcgg aaactttacc aaggtttcgg gccccgtcct ggaaatgttg
     2281 ggcatccgcg tggaagccct ggtcgatgac gcaaaccgcg atgatgtcgc cggctgggat
     2341 gaggagcaac gaaagaaatt gcaggcgatc attgcagcgc gccagccatt cctcgatttt
     2401 gttttcagcc gcgcaaacaa ggatggatcg atccagagat ttcaggtcag cggtgaaccg
     2461 atgttcaatc gatcatgcgg tttcatcgga taccgcggga tcggcgtgga actcacgcag
     2521 aaaatgtaaa acgtgagcac tccgcgcgct gcaatgttgt tagctcccgg tctaagtgag
     2581 aactatcttc tcgccgcggt gccgccggtt gaacttgcgc gcctggcagg cgaactcgaa
     2641 ctggtgccgc tggcgcttgg cgaattctct acgggcccgg tggccagttg cagcacgcgt
     2701 attttccgac tacgtcaatc gtatcgatgc actacgttac cgaatcgggt gcgtccgcgg
     2761 aaattgcggg tgtcggtaat gaaggcgtcg tgggcgtttc cctgttcatg ggaggaaaca
     2821 ccacgccgag ttcggccgtc gtgcaaaccg ccgggcaggc gtaccggctg gagcgccgcg
     2881 tactgatgga ggaattcaat cgcgggggtc cattccagaa gttgctgctt cgctatacgc
     2941 aggcactcat tacgcagatg tcgcaaaccg ccgtgtgcaa ccgccaccat tcggtggagc
     3001 agcaattgtg ccgctgg
//
LOCUS       DATMZQ010000292         3044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3574110, whole genome shotgun sequence.
ACCESSION   DATMZQ010000292 DATMZQ010000000
VERSION     DATMZQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574110"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /gene="glnE"
                     /locus_tag="VN496_09515"
     CDS             complement(<1..1468)
                     /gene="glnE"
                     /locus_tag="VN496_09515"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HXF17231.1"
                     /translation="MLSTDLPTDHPALTRASRLSRYVRDLLCAEPTLPSSVPLDRVLG
                     VEWMRTQLADQYADERTLQAALRQLRKAVMLNVITRDLEGRSGLDEVVATVTALAELS
                     LATAVLHHSRWLAEEFGNPSRNSSGAVQQLHVIAMGKLGGAELNVSSDIDIVFAYPEE
                     GNTDGPRSISNHEFFIRLARKIISAIAEVTEQGFVFRVDTRLRPYGDSGPLAVSFDML
                     EEYFTTQGREWERYAWVKARSLTGVATGALDAVIAPFVYRRHLDYSAIASLRSLHAQI
                     RQEAKKREIADNIKLGPGGIREIEFLVQVFQMIRGGREPALRLRSTLKALALLGEKNI
                     LPAAAVAELRAAYVYLRDLEHRLQYLDDQQTQILPHTEEDRGRVAEMMRCTDYAALCK
                     QLDEHRVAVSRHFEAIFAGVKEAGPAHGQADPWLLSLSADESRSRLEAMGFADAQRIN
                     ERLAGIRSSARYRRMAASSQALLDRLVPQLLETARKFSN"
     gene            1577..>3044
                     /locus_tag="VN496_09520"
     CDS             1577..>3044
                     /locus_tag="VN496_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17232.1"
                     /translation="MSELRPRILTEQRSATFVKRPTVYLKHSFTFLKHGSLWTYRVAT
                     LSVLATGLLFLSLVLGLRYYLLPHIDEYREPIARAISASIGQRVTIGSLSGSWQGYRP
                     EMSLMDVEVLGADGSVALAFERVTTVLSWLSLVSAELRFDSLAVYGPALEVKRDASGV
                     IWIAGVALKPQQNSGDGFGNWLLAQRQVLVRDAAIVWLDEMRGAPRLPFDKVDFRLDR
                     DGAIHRFGVTAMPPSHVASPFAARGQFVGSDLSDLPSWKAKVYAELDYADLAAAQTWI
                     PVPLELSSGVGSLRVWLELNGARLGAASADIRLTNVKTRLAADLPELALSEVQGRLDW
                     TQSGDRTEFSATSLAITVAGGPRLVPMQATYAYSAPAGVIRHADMHLSNLDLAAVASL
                     AEFLPIDDALRSKLARTAPAGNVQDAVLSWDGEWGTGQPYTARAKFSNVSAGPDGAFP
                     GLHGVSGQFVADERGGTASLTVSKGGFALPSVFAETIPI"
BASE COUNT          565 a          919 c          935 g          625 t
ORIGIN      
        1 gattcgagaa ttttcgcgcc gtctcgagca attgcggaac cagtctgtcc agcaaggcct
       61 ggctggaggc ggccatgcgc cgataacgcg cgctggacct gattcccgcc agccgctcat
      121 tgatgcgttg tgcatcggcg aaccccatcg cctccaggcg ggaacgcgat tcatcggctg
      181 acaacgacag tagccatgga tcggcctggc cgtgcgccgg accagcttcc ttaacgcctg
      241 caaatatggc ttcgaaatgc cgcgaaactg cgacgcgatg ctcatccagt tgcttgcata
      301 gtgcggcgta atccgtgcat cgcatcatct cggcgacgcg cccgcggtct tcttctgtgt
      361 gcgggagaat ctgtgtctgc tggtcgtcca ggtattgcag gcggtgttcg aggtcccgca
      421 agtacacgta ggcggcacgc aattccgcaa cggccgctgc aggcagaatg tttttttcgc
      481 cgagcagcgc cagggctttc agcgtggacc gcaaccgcag cgccggttcg cgcccgccgc
      541 ggatcatctg gaacacctga acgagaaact cgatctcgcg gatgccgcct ggacccagtt
      601 tgatgttgtc cgcaatctca cgctttttcg cttcctggcg gatctgcgcg tgcaagctgc
      661 gcaagcttgc gatcgcgcta tagtccagat ggcgccggta gacgaagggc gcaatgaccg
      721 cgtccagcgc gccagtcgcg acgccggtga gactgcgagc cttgacccag gcatagcgtt
      781 cccattcgcg tccctgcgtc gtgaaatatt cctccagcat gtcgaaacta acggccaacg
      841 gaccgctgtc accatagggc cgcagccgcg tatcgacccg gaaaacaaaa ccctgctccg
      901 tgacttctgc gatggcgctg atgatcttgc gcgcaaggcg gataaagaac tcatgattgg
      961 aaatggatct tggcccgtca gtgttgccct cttccgggta cgcaaaaacg atatcaatat
     1021 ccgaagacac attaagttcc gcgccgccaa gcttccccat cgcaatgacg tgcaactgtt
     1081 gcaccgcccc ggagctgttt cgcgacggat tgccgaactc ctccgcaagc cagcgtgaat
     1141 gatgcaaaac cgccgtcgca agcgaaagct ccgccaatgc agttacggtc gcgaccacct
     1201 catccaggcc gctgcgccct tcgaggtcgc gggtgatgac gttcagcatg accgctttgc
     1261 gtagctgccg cagagcggcc tgcaaagtac gctcgtccgc gtactgatca gcgagctgcg
     1321 tgcgcatcca ttccaccccg agtacccggt ccagcgggac actggacggc agagttggtt
     1381 cggcgcagag caaatcacgc acgtagcgcg acagccgcga cgcgcgagtc aatgccgggt
     1441 gatcagtcgg taagtcagta gagagcatgc gtcgttggtt tagaatgtgc gctcgatcag
     1501 cagcggctct tggcaggggc gccggcgtca acggctccgg cgaacggccg ttacacgggt
     1561 gccgcggata cgatggatgt ctgagttgcg tccgcgtatc ttaactgaac agcgtagtgc
     1621 aacgtttgta aagcgcccga ccgtgtacct caaacattcc tttaccttcc tgaagcacgg
     1681 ttctctgtgg acctatcgag tcgcaacgct gtcggtcctg gccacggggc tgcttttttt
     1741 atcgctggtt ctcgggttgc gctactacct gttgccgcat atcgacgagt atcgtgaacc
     1801 gatcgcccgg gcgatcagcg cttcgattgg ccaacgcgtc accatcggtt cactgagcgg
     1861 cagctggcaa ggctatcgcc ctgaaatgag cctgatggac gtcgaggtcc tcggtgcgga
     1921 cggcagcgtc gcactggctt tcgagcgcgt caccaccgtg ctgtcctggc tgtcgctggt
     1981 gtcggctgag ttgcgctttg attctctggc ggtctacggg ccggcgctcg aagtcaagcg
     2041 cgacgctagc ggcgtgatct ggattgctgg cgttgcgctc aagcctcagc aaaattcggg
     2101 tgatggattc ggtaactggc ttctggcaca gcgccaggtc ctcgtgcgcg atgccgccat
     2161 tgtctggctc gacgagatgc gcggtgcccc aaggctgcct ttcgacaagg tcgacttccg
     2221 gcttgaccgt gatggcgcca tacatcgctt tggtgtgact gcgatgccac cctcgcacgt
     2281 tgcatcgcca ttcgctgcac gcggccagtt tgtcggcagc gacctcagtg acttgccatc
     2341 gtggaaagcg aaggtctatg ccgagctgga ctatgcagac ctggcggcgg cacagacctg
     2401 gataccggtt ccgctggagc tgtccagcgg tgtcggttca ttgcgcgtgt ggctggaact
     2461 gaacggcgcg cgtctgggcg cggccagcgc agatatcagg ttgaccaatg tcaaaacgcg
     2521 actggctgcc gatcttcccg aactcgcatt gtcggaagtg cagggaaggc tggactggac
     2581 gcaaagcggt gatcgcaccg aattcagcgc gacttcgctt gccattactg ttgcgggcgg
     2641 cccgaggctg gtaccgatgc aggccactta tgcctattcg gcaccggcag gcgtgatacg
     2701 ccatgccgac atgcatttgt ccaatctcga tctcgcggca gtggccagtc tggccgagtt
     2761 cctgcccatc gacgatgcgc tgcgcagcaa gctggcacgc acggccccgg ccggtaatgt
     2821 gcaagacgcg gtcttatcct gggacgggga atggggcacg gggcaaccct atacggcaag
     2881 agcaaaattt agcaatgtct cggcgggccc agatggagcg ttccccggct tgcatggtgt
     2941 gagcggtcag ttcgtcgcgg atgagcgtgg cggaacggcc tcgcttacag tatccaaagg
     3001 cggttttgcg ctgccgagcg tattcgctga gacgattccg atcg
//
LOCUS       DATMZQ010000293         5334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1968671, whole genome shotgun sequence.
ACCESSION   DATMZQ010000293 DATMZQ010000000
VERSION     DATMZQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968671"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VN496_09525"
     CDS             <1..667
                     /locus_tag="VN496_09525"
                     /inference="COORDINATES: protein motif:HMM:NF023592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HXF17233.1"
                     /translation="GLAPDEFAGFTMTLMRMLAFVPMEAGGSVPTGVSNRPQAGAKAR
                     PAPDSVARSVTTAVPDQVVPPNRSLERAAAPASPLLPAMEQPAQPARDPTIGEQLSDW
                     RNLVGRIKVGGMARMLGDNCEFQSLQGDKVELVVPEAHKHLLEKAYTEKLQSALAEYF
                     DCKLRLRISIGGSGKTPAEIENQERQVKLAQAIQSIDADPFVRELVENFDARIRDTSI
                     KPV"
     gene            683..1009
                     /locus_tag="VN496_09530"
     CDS             683..1009
                     /locus_tag="VN496_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXF17234.1"
                     /translation="MMKGQLAGLMKQAQQMQENLKKAQDEIAAMEIEGQAGAGMVKVT
                     MTGRHDVKRVSIDPSLMGDDKDMLEDLIAAAINDAVRRVESMTQEKMGGLTSGFGLPP
                     GMKLPF"
     gene            1107..1703
                     /gene="recR"
                     /locus_tag="VN496_09535"
     CDS             1107..1703
                     /gene="recR"
                     /locus_tag="VN496_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXF17235.1"
                     /translation="MKTPSTVEELIEALRCLPGVGPKSAQRMAFHLLQRDQPGAQRLG
                     RALEQALSKVRHCERCNNFTEQVVCELCSSPRRDSTSLCVVETPADLLMMEQAQAYDG
                     MYFVLMGRLSPLDGVGPKDIHVDRLVKRALDGIVQETILATNFTVEGEATAHYLGELL
                     GTRGMKVSRIARGLPVGGELEHVDSGTLAQAIIERRAF"
     gene            1703..2695
                     /locus_tag="VN496_09540"
     CDS             1703..2695
                     /locus_tag="VN496_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXF17236.1"
                     /translation="MAAESGAKASSRRSSPRRAPKSTPPVAKGSRRPARPVAKRDLAV
                     PPMLPAPAPAVPRSQKLQKVLAQSGLGSRRAMEEVIKAGKVKVNGEPAALGTRVTTED
                     MIQVGRRQIKFKVTSRLPRVIIYHKMDGEIVSRDDPQGRRSVFEKLPVIRSAKWIAVG
                     RLDFNTGGLLIFTTSGELANRLMHPRFEVEREYAVRLFGALSPAHVQELKTGVRLSDG
                     DARFEVLDDQGGEGRNRWYRVVLKEGRNRVVRRMFEALGLQVSRLMRVRFGIVTLPPR
                     PKRGNWIELKEAEISKLLEWAGSPAEAADDDLRVADPPRPAERPSRHRPRSPAR"
     gene            complement(2625..3518)
                     /gene="prmB"
                     /locus_tag="VN496_09545"
     CDS             complement(2625..3518)
                     /gene="prmB"
                     /locus_tag="VN496_09545"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXF17237.1"
                     /translation="MAAEAGRGPNKVRDWLNLTVDRFRNTGLHFGHGSSNARDEAAYL
                     LLHTLNLPLHELDSVLDRPLTRDEINALEHIVRRRVDERIPAAYLTREAWLGECRFYV
                     DERTIVPRSYIAELLPERLHPWVVDEQTIASALDLCTGSGCLAILLALAFPNARVDAT
                     DLSADALSVAETNIAEYDLRDRVHLTRGDLFAGLAGRYDLIVCNPPYVNQKTMARLPP
                     EYRAEPVMALAGGQDGLDFVRRVLEQARVHLTERGLLIMEIGHNREALEQTFPRLPFI
                     WLETSGGGDLVFLLGAEDLPL"
     gene            complement(3508..4686)
                     /gene="dapE"
                     /locus_tag="VN496_09550"
     CDS             complement(3508..4686)
                     /gene="dapE"
                     /locus_tag="VN496_09550"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HXF17238.1"
                     /translation="MTKRADRAEATQSVSASPTLNLARELISRVSITPNDAGCQQLLA
                     QRLSQLGFTIEHLRFGDVDNLWARHGNSAPVICFAGHTDVVPTGPLAQWHSDPFVPTV
                     RDEYLYGRGAADMKCSIAAFVTAVEAYLGQHKQHPGSIGLLITSDEEGVAVDGTVKVV
                     DALRTRGETIDYCIVGEPSSVSRLGDTMKNGRRGSLSGELMVRGIQGHIAYPHLSRNP
                     IHEFAPALSELSAAKWDDGNEYFPPTTWQVSNITAGTGATNVIPGELRVSFNFRFSTA
                     STAETLKARVHEVLDRHGLDYALTWSLSGQPFLTPKGKLVETLSRAVQTVVGYTPELS
                     TSGGTSDGRFIADICREIVELGPINASIHKLNEHIALADLEALSQIYRLALADILNGR
                     "
     gene            complement(4670..>5334)
                     /locus_tag="VN496_09555"
     CDS             complement(4670..>5334)
                     /locus_tag="VN496_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXF17239.1"
                     /translation="KCVINQREVGTDTRTYENALVNAMREAPDLMMIGEIRDRETLQH
                     ALLYAQTGHLCISTLHANNSYHALNRIINFFPYDARTSLLSDLSISLRAIISQRLVKA
                     NDGTLVAAVEVMLNTKHIAELIKKGEIDQIREAMEQSLSPGSKTFEHALFELYMSGTV
                     SKDEALKNSDSSTNLSWLINNFEQKAAGKLGESPTGRVSIKPGIGYSFNEIKLNVDDE
                     KS"
BASE COUNT         1148 a         1480 c         1622 g         1084 t
ORIGIN      
        1 gggcctcgcg cccgatgaat tcgccggctt taccatgact ttgatgcgca tgttggcctt
       61 tgttccgatg gaggctggag gcagcgttcc taccggcgta tcaaatcggc cgcaggccgg
      121 tgccaaggcc aggcctgcgc cagattctgt ggcgaggagc gttacaacgg cagtgccgga
      181 ccaggtagta ccgccgaaca ggagtctgga gcgcgccgcc gcacctgcat cgccgctgct
      241 tccggcgatg gagcagcctg cgcaacccgc tcgggacccg acgatcggtg agcagctgtc
      301 cgactggagg aatctggtgg gccgcatcaa agtaggcggc atggcgcgca tgctgggcga
      361 caattgtgaa ttccagtccc tgcaaggtga caaggtggaa ctcgtcgtgc cggaggcaca
      421 caaacacttg ctcgaaaaag cctacacgga aaaattgcaa tcagcgctcg ccgagtattt
      481 cgactgcaag ctgcggctgc gtatttcgat cgggggcagc ggcaagacac ccgccgaaat
      541 tgaaaatcag gagcgccagg tcaaactcgc ccaagccatc cagtcgatcg atgctgatcc
      601 gttcgtacgc gaactggtcg agaattttga tgcccggatc agggatactt ccataaaacc
      661 ggtttagttc aaggataagc acatgatgaa aggtcagctc gccggcctca tgaaacaggc
      721 ccagcaaatg caggaaaatt tgaagaaggc gcaggatgaa attgccgcga tggaaattga
      781 agggcaagcc ggtgccggca tggtcaaagt aacgatgacc ggacgccatg atgtcaaacg
      841 cgtaagcatc gatccgagtc tgatgggcga tgacaaagat atgctcgagg atctcattgc
      901 agccgcaatc aatgatgccg tgcgccgcgt ggaatccatg acgcaggaga agatgggcgg
      961 cttgacgtcg ggcttcgggt taccgcccgg catgaagctg ccgttctagt aaaacagtaa
     1021 atggtgaata gtgaaaggtg taaacattgc ggcaggactg tttgcagttc accgatcacc
     1081 gtttgccgac caccgttcac cccccaatga aaacgccgtc caccgtcgaa gaactgatcg
     1141 aagctctgcg ctgtctgccc ggcgtcgggc cgaaatcggc gcagcgcatg gcttttcacc
     1201 tgctgcaacg cgatcagccc ggtgcgcagc ggctcggacg cgcgctggag caggcactgt
     1261 cgaaagtcag gcattgcgaa cgttgcaata actttacgga gcaggtggtg tgcgagttgt
     1321 gcagttcgcc gcgccgcgac tcgacctcgc tgtgcgtagt cgagacgccg gccgatctgc
     1381 tgatgatgga acaggcgcag gcttatgacg gcatgtattt cgtgttaatg ggccggctct
     1441 cgccgctcga tggcgtcgga ccaaaggaca ttcacgtcga tcgcctggtc aagagggcat
     1501 tggacggcat cgtgcaggaa accattctcg cgaccaattt caccgtcgaa ggagaagcca
     1561 ctgcgcatta tttgggtgaa ttactcggca cgcgcggcat gaaagtttcg cgcattgcac
     1621 gcggacttcc agttggtggc gagctggagc acgtcgacag cggcaccctg gcacaggcga
     1681 tcatagagcg gagggcgttc tgatggccgc cgaatccggg gcaaaggctt cttcgcgcag
     1741 gtcttcgccg cgacgcgcgc cgaagagtac gccgccggtc gccaagggat cgagaaggcc
     1801 ggcacggccg gtggcaaagc gtgatcttgc ggtgccgccc atgttgcctg cacctgctcc
     1861 ggctgtgccg cgttcccaga aattgcagaa ggtactggcg caatccgggc tgggctcgcg
     1921 gcgtgcaatg gaagaagtca tcaaggccgg caaagtgaag gtaaatggag aaccggccgc
     1981 actcggcacg cgcgtgacta ccgaggatat gatccaggtc ggacgccggc agatcaaatt
     2041 caaagtcact tcgcggctgc cacgggtaat tatttatcac aagatggatg gagaaatcgt
     2101 cagccgcgat gatccgcagg gtcgccgcag cgtgttcgaa aaactgcccg tcatacgcag
     2161 tgccaaatgg attgcggtgg gccgcctcga tttcaatacc ggggggttat tgatttttac
     2221 cacttctggt gagttggcca accggttgat gcatccccgc tttgaagttg agcgcgaata
     2281 cgccgtgcgc ttgttcggtg cactatcacc tgcgcacgtg caggaactca agaccggcgt
     2341 gcggctttcc gatggtgacg ccaggttcga agtgctggac gatcagggcg gcgaaggccg
     2401 caaccgctgg tatcgcgtgg tattgaagga gggcaggaac cgggttgtgc gccgcatgtt
     2461 cgaagcgctg ggattgcagg tcagccggct gatgcgtgta cgcttcggta tcgtgacctt
     2521 gccgccaaga cccaagcgcg gcaactggat cgaactcaag gaagcagaga tcagcaaatt
     2581 gcttgaatgg gccggcagtc cagctgaagc ggccgacgat gacctcagag tggcagatcc
     2641 tccgcgccca gcagaaagac caagtcgcca ccgcccgagg tctccagcca gataaatggc
     2701 aggcgcggga aggtttgctc cagtgcctcg cggttatggc caatctccat gatcaacaat
     2761 ccgcgctcgg tcagatgtac gcgcgcctgt tccagcacgc ggcgcacgaa atcgaggccg
     2821 tcctgacctc cagcgagcgc catgacaggc tccgcgcgat attccggcgg taaacgcgcc
     2881 attgtcttct ggttgacgta tggcgggttg caaacgatca agtcgtagcg ccctgcgagc
     2941 ccggcaaaca aatcgccgcg ggtgaggtgc acccggtcgc gtaaatcata ttcggctatg
     3001 tttgtctcgg cgacggatag tgcatcggcc gacagatcgg tcgcatcgac tcgtgcgttt
     3061 ggaaaggcaa gtgccagcag gatggccaga caacccgatc cagtgcacaa gtccagtgcg
     3121 cttgcgatgg tttgctcatc gacaacccat ggatgcaagc gctcgggcaa cagttcggca
     3181 atgtaggaac gcggtacgat agtgcgctcg tccacgtaaa atcggcattc cccaagccac
     3241 gcttcacgcg tcaggtaagc agccggaata cgctcatcga cccgtctgcg gacgatgtgc
     3301 tccagcgcat tgatttcgtc gcgggtcaat ggacggtcga gtaccgagtc cagctcgtgg
     3361 agcggcaaat tgagcgtatg caacagcagg tacgcggctt cgtcgcgcgc attgctgctg
     3421 ccatggccga aatgcaaccc ggtattcctg aaacgatcaa ccgtgagatt caaccagtcg
     3481 cgaaccttgt tcggtccgcg cccggcttca gcggccattc aatatgtcgg cgagcgccaa
     3541 tcggtagatt tgggacagcg cttccagatc cgccaatgcg atatgctcgt tgagtttatg
     3601 gatcgatgcg tttatcggcc cgagttccac gatttcgcgg caaatatcag cgatgaagcg
     3661 gccgtccgaa gttccgccac tggtggacaa ctcaggcgtg tagcccacca cagtttgcac
     3721 tgctcgcgac aaagtctcca cgagtttgcc cttgggggtc agaaacggct gaccggagag
     3781 cgaccatgtc aatgcatagt cgaggccatg gcggtcgagt acttcatgta cgcgcgcttt
     3841 gagcgtctcg gcggtgctgg ccgtggagaa cctgaaatta aacgacacgc gcaactcgcc
     3901 cggaatcaca tttgtggcac ccgtgccggc agtgatgtta gaaacttgcc aggtggtcgg
     3961 cggaaaatat tcgttgccat cgtcccattt tgctgccgac agttcggata gcgctggcgc
     4021 gaactcatga atgggattgc gcgagaggtg tggataggcg atatgtccct ggataccgcg
     4081 caccatgagt tcgccagaca gcgagccacg gcgtccattt ttcatcgtgt caccgagccg
     4141 ggagactgac gatggttcac cgacaatgca gtaatcgata gtctcgccac gcgtgcgcaa
     4201 cgcatcgacc accttgacgg tgccgtccac ggcaacgcct tcctcatcag atgtgatcag
     4261 taacccgatt gaacccggat gctgcttatg ctgacccaga taggcttcga cggcggtaac
     4321 gaaggccgcg attgagcact tcatgtccgc ggcgccgcgc ccatacaaat attcatcgcg
     4381 caccgtgggc acaaatggat cggagtgcca ttgtgcaagc ggcccggttg gaacgacgtc
     4441 ggtgtgtccc gcgaaacaaa ttacaggcgc gctgttgccg tgacgcgccc acaggttatc
     4501 gacgtcgcca aaacgcaggt gttcgatcgt gaaaccgagt tggctcaggc gctgcgccaa
     4561 cagttgctgg caacctgcat cattcggcgt aatcgaaacg cgcgaaatca gttcgcgggc
     4621 gagattcagc gtcggggatg cactgaccga ttgcgtagct tcggctctgt cagctctttt
     4681 cgtcatccac attcagcttg atctcgttga acgaataacc gatgccgggc ttgatactga
     4741 cgcgtccggt cggactttct ccaagtttgc cggctgcctt ttgttcaaag ttgttgatga
     4801 gccaggacag gttcgtcgac gaatctgaat tcttcagggc ttcatctttt gacaccgtgc
     4861 cggacatgta aagctcgaac aacgcgtgct caaatgtctt cgagcccggg gacagacttt
     4921 gttccatggc ttcgcgtatc tggtcgattt cgcctttctt gatcagttca gcgatatgct
     4981 tggtattgag catgacttcg accgctgcca ccagcgtgcc gtcgtttgcc ttgaccaggc
     5041 gctgcgaaat gatcgcgcgc aggctgatgg aaagatccga cagcagcgaa gtacgggcat
     5101 cgtacggaaa aaagttgatg atccgattca gcgcgtggta actgttgttg gcgtgcagcg
     5161 tcgagatgca aaggtggccg gtctgtgcat agaggagtgc atgctgcagc gtctcccggt
     5221 cgcggatttc tccgatcatc atcaggtcgg gtgcctcacg catggcattg accagcgcat
     5281 tttcgtaggt cctggtatcg gtgccgactt cccgttgatt gatgacgcat ttgt
//
LOCUS       DATMZQ010000294        15704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_587263, whole genome shotgun sequence.
ACCESSION   DATMZQ010000294 DATMZQ010000000
VERSION     DATMZQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587263"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /gene="cmk"
                     /locus_tag="VN496_09560"
     CDS             <1..1458
                     /gene="cmk"
                     /locus_tag="VN496_09560"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXF17240.1"
                     /translation="FLTALLMALPLAGRQITIEVLGELISKPYIDITLNLLSRFGVNV
                     QREGWASFKIPAKSKLVSPGEIFVEGDASNASYFLAAGAIGVGPVRVEGVGSSSIQGD
                     VGFANALAVMGAQISMGEHWIESSAPSSGRLRAFDLDLNAIPDAAMTLAVAALFAQGT
                     SRLRNIGSWRVKETDRIAAMAKELRKLGANVEEGADFLTVTPPTDSSLTNGAVIDTYD
                     DHRMAMCFSLAALGKVMVRINDPNCVAKTFPDYFERFAEVTWNSSTPVIAIDGPSASG
                     KGVVAQRVAEHLGFHYLDSGALYRLVAFNALRENVRLNDEKRLGEIARGLPARFKGGR
                     ILLHEEDVTNAIRTETVAAAASEVAPIVMVRQGLKQRQQAFRRPPGLVAEGRDMASVV
                     FPDALLKVFLTASAEERADRRYKQLIEKGFDANLTTLLQDIRQRDARDSERSVAPLQK
                     SVDAQIIDTTGLSVEQVVARILQWFGSALRKAPSR"
     gene            1581..3305
                     /gene="rpsA"
                     /locus_tag="VN496_09565"
     CDS             1581..3305
                     /gene="rpsA"
                     /locus_tag="VN496_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXF17241.1"
                     /translation="MKNESAAPASPINENSFAALFEESLSRQEMRAGEVITAEVVRID
                     QNFVVVNAGLKSESFISADEFRNDRGEVEVNVGDYVTVAIEALEDGYGETRLSREKAK
                     RLAAWHDLEKAMEEGTLVQGLVNGKVKGGLTVMVNGIRAFLPGSLVDIRPVKDTNPYE
                     GKQLDFKVIKLDRKRNNVVVSRRAVLEQTQGAERDALLASLQEGAVVKGIVKNITDYG
                     AFVDLGGIDGLLHITDLAWRRVKHPSEVLNVGDEVTAKILKFDQEKNRVSLGLKQLGE
                     DPWVGLSRRYPTGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSRVV
                     QLGDEVEVMILEIDEDRRRISLGMKQCKPNPWDEFASNAKKGDRVRGQIKSITDFGIF
                     IGLQGGIDGLVHLSDLSWSVPGEEAVRNYKKGDEVEAMVVSIDVERERISLGIKQLEG
                     DPFTNFVAGHDKGSMVDGVVKSIDAKGAVVQLDVDVEGYLRASELARDRVEDIRTHLK
                     EGDKVNAMIINIDRKNRNINLSIKAKDAADESEAMQKHASDSASNAGTTNLGALLKAK
                     LDNKNSAQ"
     gene            3314..3601
                     /locus_tag="VN496_09570"
     CDS             3314..3601
                     /locus_tag="VN496_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HXF17242.1"
                     /translation="MTKSELIAKLAAHFPQLVASDAELAVKMILDAMAKSLSQGHRIE
                     IRGFGSFGLNYRPPRVGRNPKSGDKVHVPAKYVPHFKAGKELRERVDFKSQ"
     gene            3702..3983
                     /locus_tag="VN496_09575"
     CDS             3702..3983
                     /locus_tag="VN496_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXF17243.1"
                     /translation="MILRMRYLSWAFRLLLFILLLGFALKNSDPVSVRFYLGARWDAP
                     LALVAFVFFGMGAAAGVAACFIYIYRLRGEILQLRKELRAKIAAPEDAL"
     gene            4026..5201
                     /gene="lapB"
                     /locus_tag="VN496_09580"
     CDS             4026..5201
                     /gene="lapB"
                     /locus_tag="VN496_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HXF17244.1"
                     /translation="MEFEFWWLLGFPLFFAFGWLAARIDIKQLVSESRSLPLSYFKGL
                     NFLLNEQPDKAIEAFIELVKVDPQTIELHFALGSLFRRRGEVERAIRMHENLLERADL
                     APEQKMTALYELAQDYLKAGLLDRAEELFAKLKGTLYEQQGLKFLLEIFEQEKDWSKA
                     IETSRKMDSVSGQSSAKDIAHFYCELAAVEITHSRWDQARDYLSEALSVNRRCARANI
                     LLGDTEMGEGRPAAAIEYWKALESQNPAYIALVAEKILAAYRSLGRDEEGLQLLRGYL
                     TNYPSLDLLNVIFQGELDHLGPEHAYRLVRNELARNPTLLGLDKLLEAQLLEAPVERR
                     HELQLIKNLVHQHTRSLALYRCESCGFKARQFYWHCPACNRWETYPPRRTEEREFGA"
     gene            5198..5905
                     /gene="pyrF"
                     /locus_tag="VN496_09585"
     CDS             5198..5905
                     /gene="pyrF"
                     /locus_tag="VN496_09585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXF17245.1"
                     /translation="MNSRIIVALDYADARSATALVDRLNPAACKLKIGKELFTAAGPS
                     FVAMIAQRGFEIFLDLKFHDIPNTVAQACKAAAQLGVWMINVHALGGRAMMNAAREGI
                     ESAAHKPKVIAVTVLTSMAETDLREVGIDSNPTAEVMRLAELASDCGLDGVVCSAWEA
                     SAIRDQLGSRFLRVTPGIRLPEEAADDQKRIMTPQLAIRSGSSYLVVGRSVTRSTDPL
                     AVLARINGEISVPEVQQ"
     gene            5902..7227
                     /locus_tag="VN496_09590"
     CDS             5902..7227
                     /locus_tag="VN496_09590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXF17246.1"
                     /translation="MKITVIGTGYVGLVSGACFADLGNDVVCLDVDDKKIAQLKSGGI
                     PIYEPGLQQLVNHNVAAGRLRFTTDVEESVTHGAVQFIAVGTPSDQDGSADMRYVLAA
                     ARGIGRTMTEYRVIANKSTVPVGTSERVRSAIAEELKKRSLDLPFSVVSNPEFLKEGA
                     AVGDFMRPDRIVVGSSDERATQLMRQLYTPILRNHDRILIMDPRSAELTKYAANAMLA
                     TRISFMNELANLAEKLGANIESVRHGIGSDQRIGYHFLYAGVGYGGSCFPKDIQALQH
                     TAGEYGVQLTIVDSVEKANRTQKQRILEKVRSRFGEQLDGRRFAIWGLSFKPNTDDMR
                     EAPSRVIINGLLARGASITAYDPVAMEEAKKIFAKESGVRFASSTVGALDGADALIIV
                     TEWKEFRSPDFEDMKYRLKSPVVFDGRNLYDPAMMRDAGIEYFSVGRPQ"
     gene            7224..8150
                     /gene="rfaE1"
                     /locus_tag="VN496_09595"
     CDS             7224..8150
                     /gene="rfaE1"
                     /locus_tag="VN496_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HXF17247.1"
                     /translation="MIGREAVKRARVLVVGDVMLDRYWFGEVERISPEAPVPVVKVGR
                     TEERLGGAANVARNIVAVGAAAGLLSVVGEDEAAACIQSLVKADGIDAQLHRDPALHT
                     TIKLRVIGRQQQLLRIDFETEPGHEVLMSKLADFEARLKNADLVILSDYGKGGLKHIE
                     RMISAARAAGKAVLVDPKGDDYSRYRGATLLTPNRSEFREVAGRWKNEEDMTERANKL
                     RAELGLDALLITRSEEGMTLYRQGERLHVPAVAREVYDVSGAGDTVIAVLGVMLAAGA
                     GFADAVSFANKAAGIVVGKLGTAVVHPEELFH"
     gene            8160..9155
                     /gene="rfaD"
                     /locus_tag="VN496_09600"
     CDS             8160..9155
                     /gene="rfaD"
                     /locus_tag="VN496_09600"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HXF17248.1"
                     /translation="MYIVVTGAAGFIGSNLVKALNARGETNILAVDNLSRAEKFRNLV
                     DCEIADYLDKEHFRAALDAGSFDGEIVAVLHQGACSDTMQTDGRYMMDNNFHYSSDLL
                     AYCQSESVPLIYASSASVYGAGPIFKEGRANERPLNIYGYSKFLFDQLVRRRWEDNSA
                     QVVGLRYFNVYGPRETHKGRMASVAMHCFDQFREGGKVKLFEGSGGYAAGEQRRDFIS
                     VDDVVNVNLHFLDHPAQSGIFNCGTGASQTFNDVAAATINALRVAEGKNEMAIAELQA
                     QGLVEYGPFPPALKDKYQSFTEANLSALRGAGYAAPFADVRTGVGRYVQWMLKNR"
     gene            9170..10105
                     /gene="cysM"
                     /locus_tag="VN496_09605"
     CDS             9170..10105
                     /gene="cysM"
                     /locus_tag="VN496_09605"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HXF17249.1"
                     /translation="MGTQANLRRDRDRAGSGKTLDDFVGNTPLVRLKRIPGNTTNVIL
                     AKLEGNNPAGSVKDRPALSMIRKAEERGRIKAGDTLIEATSGNTGIALAMVAAMRGYR
                     MILVMPEHLSLERRQSMAAFGAKFVLTPKQGGMEKARDVAEQMQAAGAGLILDQFANP
                     DNPLAHYEGTGPEIWRDTDGQITHFVSSMGTTGTIMGCSRYFKEKNPAIAIIGCQPTE
                     GSQIPGIRKWPHAYMPRIFEPSRVDRVVEIAQEDAEDMTRRMAREEGIFAGVSSGGAL
                     WAALQISAETRNAIIVTIVCDRGDRYLSTGIFPAE"
     gene            complement(10125..11972)
                     /locus_tag="VN496_09610"
     CDS             complement(10125..11972)
                     /locus_tag="VN496_09610"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HXF17250.1"
                     /translation="MSRGNQAALPGHPDFVQNAGLLDWVAEVARLTQPQNIHWCEGSQ
                     GEYEMLCSRLVAAGTLRRLNALKRPGSYLALSDPSDVARVEDRTFICSEREQDAGPTN
                     NWTEPRKMKRTLQSLFEGSMRGRTMYVVPFSMGPIGSPLSLIGVQITDSAYVAVSMRI
                     MTRMGRQVIDALGADGEFVPCLHSVGMPLAHGQADVAWPCNSDHKYIVHFPEENAIWS
                     FGSGYGGNALLGKKCLALRIASNIGRREGWLAEHMLILGVESPRGSKTYVAAAFPSAC
                     GKTNFAMMIPPKAFEGWKITTVGDDIAWIKPDAEGRLRAINPEYGFFGVAPGTSEKSN
                     PNAMAMLRANCIFTNVALTPDGDVWWEGMTDEPPDYLTDWQGKPWTPGSGRPAAHPNA
                     RFTVPAAQCPSLDPDWENPEGVPIAAFIFGGRRSNTVPLVMQAFNWTDGIYMAATMGS
                     ETTAAATAKVGEVRRDPMAMLPFCGYHMGDYFSHWLRLGRSIERPPSIFCVNWFRKDA
                     KGKFLWPGYGENMRVLKWIVDRCEEKAGAQESPLGWMPRQDDLEWSGLEFPTERFTEL
                     MRLNKDGWTRELALHDELFAKLSERLPQEFALKRESLLSSLKHATQYWT"
     gene            12228..13010
                     /locus_tag="VN496_09615"
     CDS             12228..13010
                     /locus_tag="VN496_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXF17251.1"
                     /translation="MTPILAFDIETVPDVSGLRKLYGHAPDTPDGEVAQIAFDRKREA
                     TGSDFLPLHLHRVVAISCALRDRDSFRVWSLGESGESEAELLQRFFDGIEKYTPQLVS
                     WNGSGFDLPVLNYRALIHGVHAARFWDLGEEDRDFRWNNYIGRYHSRHLDLMDVLAMY
                     QGRGNAPLDQMAQLLGLPGKLGLDGSKVWDAFQQGHIGAIRSYCETDVANTYLLYLRF
                     QLVRGITDTVAYRRECDLVRATLLKSGEIHWREFLAGWADEG"
     gene            13000..14313
                     /gene="rlmD"
                     /locus_tag="VN496_09620"
     CDS             13000..14313
                     /gene="rlmD"
                     /locus_tag="VN496_09620"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HXF17252.1"
                     /translation="MRAEPIARFAQVESLDQEGRGIAHVEGKAIFITGALPGESVEYV
                     PYQRKPNYELADTTRILRASPSRVIPRCPHFGVCGGCSLQHMDLRAQVAAKQRILEDN
                     LARIGKVRAQIMLSPILGPDWRYRYRARLSARYVEKKSRQLLGFHERRSSFVADMGSC
                     EVLPARISALLEPLRELIGSLSIRERVPQVELAIGDAVDVLVLRVLEAPRSDDAEKLR
                     SFAEQYKIQFFLQPGGPDTARAYYPQDAAVLQYRIPDFDLTFPFHPTDFTQVNHDINR
                     VLVRRALMLLDPQPGERIGDFFCGLGNFSLAIARRGAQVLGIDGNARLALRAKETALL
                     NGVADQCSFIDRDLFKITEDEWKKIAPFDRLLLDPPRDGAMELVKALGQMAPGRVVYV
                     SCNPATLARDAGVLVNVHGYQLSAAGVINMFPHTSHVESIAVFDR"
     gene            complement(14372..15058)
                     /locus_tag="VN496_09625"
     CDS             complement(14372..15058)
                     /locus_tag="VN496_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HXF17253.1"
                     /translation="MQPEIQYGSQPGALSVQQNKVLRNTYLLLALTMVPTVIGAFVGL
                     STSGIILQHPIMATLLMLGAIIGLQFAIAANRNSGLGVALLLGMTFVLGWWLGPLLNF
                     ALRLQNGPQLIGIAAIGTGGILVAMSLIATTTKKDFSFMGKFLFVGMIVLLLAMLANM
                     FFQIPALALTISALVVVVFSLFLLHDVSRIVTGGETNYIMAATGVYISLYNIFVNLLQ
                     LLMAFTGQRD"
     gene            15433..15522
                     /locus_tag="VN496_09630"
     tRNA            15433..15522
                     /locus_tag="VN496_09630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15467..15469,aa:Ser,seq:tga)
BASE COUNT         3467 a         4259 c         4618 g         3360 t
ORIGIN      
        1 ttcctgactg cgctgctcat ggcattgccg ctggctggca ggcaaatcac catcgaagta
       61 ctcggtgaat tgatatcgaa gccttatatc gacatcacac tcaacttgct gtcgcggttt
      121 ggtgtgaatg ttcagcgtga aggctgggcc agtttcaaga ttccggcaaa gagcaaactg
      181 gtcagtccag gcgagatctt cgtcgaaggc gacgcctcaa acgcgtcgta ttttcttgca
      241 gcaggcgcga tcggcgttgg gcccgtgcgc gtggaaggcg tgggcagttc gagcattcag
      301 ggcgatgtcg gtttcgccaa tgcgctcgcg gtaatgggag cgcaaatttc catgggcgaa
      361 cactggattg aatcatccgc accttcgagc ggacgtctgc gtgctttcga tctcgatttg
      421 aacgccatac ccgacgcggc catgacattg gcggtggccg cgttattcgc gcaaggcaca
      481 agccggctgc gcaacatcgg cagctggcgg gtgaaggaaa ccgatcgaat cgcagccatg
      541 gccaaggagt tgcgcaagct gggcgcgaat gttgaagaag gtgcggattt cctgactgtc
      601 acgcctccga ctgactcatc tctcaccaac ggagcggtta tcgacactta cgatgatcac
      661 cggatggcca tgtgcttttc gctcgcagcg cttggaaaag tcatggtgcg catcaatgat
      721 ccgaattgcg tcgcaaaaac ctttcctgac tacttcgaac gcttcgccga ggtgacgtgg
      781 aattcctcca cgccagtaat tgcgattgac gggccgtctg cttccggcaa aggcgtggtg
      841 gcgcagcgag tcgcggaaca cttggggttt cattatctcg atagcggcgc gttatacagg
      901 ctcgttgctt tcaacgcgtt gcgggaaaat gtgcggttga acgacgaaaa acgactgggc
      961 gaaattgcgc gcggtcttcc tgcacgcttc aaaggcggtc ggatcctgtt gcatgaagag
     1021 gacgtaacga atgcgatccg gactgaaact gttgccgccg ctgcctcaga ggttgcacca
     1081 attgtcatgg ttcgccaggg acttaagcaa aggcaacaag ccttccgccg gcccccgggg
     1141 ctggttgcag agggacgcga tatggcatcc gtagtgttcc cggacgcgtt actgaaggtc
     1201 tttctgaccg cctctgccga agaacgcgcc gatcgtcgct ataaacagtt gatagaaaaa
     1261 gggttcgatg ctaacctgac cacccttttg caggacatcc gccaacgcga cgcgcgcgat
     1321 agtgagcgta gcgtggcacc cctgcagaag agcgttgacg cacaaattat tgataccact
     1381 gggctttcgg tagaacaagt ggttgcccgg atactccagt ggttcggatc ggcgttacgc
     1441 aaggccccat cccgctgaac gattcacccg catctgctgg attctgcagc agcaacgcag
     1501 ggaggttttt ccgctgttga tgggtagata aaccaacccg ttaaatgcaa cggtatttcc
     1561 gcgagtccaa ccctttgctc atgaaaaacg aatccgcagc tcccgcttcc ccgatcaacg
     1621 agaatagttt cgccgcgctg tttgaagaaa gtctttcgcg gcaggaaatg cgtgcgggag
     1681 aagtcatcac ggccgaagtt gtccgaatag accagaactt cgttgtcgtc aatgccggtt
     1741 tgaagtccga gagttttatc tcggcggacg aattcagaaa cgaccgtggc gaggtcgaag
     1801 tcaatgttgg agattacgtc acagtcgcca tcgaagcgct tgaggacggc tacggtgaaa
     1861 cccgtctgtc ccgtgagaaa gcgaagcgcc tcgcagcgtg gcatgacctc gagaaagcca
     1921 tggaagaggg cacgctggtc cagggcctcg tgaatggcaa ggtcaaaggt gggcttacgg
     1981 tcatggtcaa cgggatccgc gcttttcttc ccggctcgct ggttgatatc cgtccggtga
     2041 aggacaccaa cccctacgaa ggcaaacaac tcgatttcaa ggttatcaag ctcgatcgca
     2101 agcgcaacaa cgtagtggtt tcgcgccgcg cggttctcga gcagactcag ggcgctgaac
     2161 gcgatgcact gcttgccagc ctgcaagaag gcgcagtagt caaaggcatc gtcaagaaca
     2221 ttaccgacta tggcgcgttt gtcgatcttg gcggcataga cggcctgctg catatcacgg
     2281 atctcgcgtg gcgccgtgtc aagcatcctt cggaagtact caatgtcggc gacgaagtca
     2341 ccgcaaagat cctgaaattc gaccaggaga aaaatcgggt atccctcggc ctgaagcagc
     2401 ttggcgaaga tccgtgggtc ggtctgtccc gacgctaccc gacgggtact cgtttgttcg
     2461 gcaaggtttc aaatttgact gactacggag cgttcgtcga aatcgagcag ggaattgaag
     2521 gactggtcca cgtttccgaa atggattgga ccaataagaa cgttcatccc tcgcgcgttg
     2581 ttcaactggg ggatgaggtc gaagtaatga ttcttgagat tgacgaagac cgccgccgta
     2641 tctcgctggg catgaaacag tgcaaaccca atccctggga cgaatttgct tcgaatgcca
     2701 aaaaaggcga tagagttcgc ggccagatca aatccattac cgacttcggg attttcatcg
     2761 gcttgcaggg cggcatcgac ggtttggtgc acttgtctga tctgtcatgg agcgtccccg
     2821 gcgaagaagc cgtgcgaaat tacaagaagg gcgacgaagt tgaggcgatg gttgtatcca
     2881 tcgatgtcga gcgggagcgt atttccctcg gtataaagca actcgaaggc gatccattca
     2941 ccaactttgt ggccgggcat gacaaaggca gcatggttga tggtgtcgtg aagtcaatag
     3001 atgccaaggg cgctgtcgtt cagcttgatg tcgacgtcga gggatatctg cgtgcttcag
     3061 agttggcgcg agaccgtgtc gaggacatcc gtacccacct caaagagggg gacaaggtca
     3121 atgcgatgat catcaacatc gatcgcaaga accgcaacat caacctttcg atcaaggcca
     3181 aggatgcggc cgacgagtct gaggccatgc aaaaacatgc ttcggacagc gcgtcgaatg
     3241 ccggcacgac gaatctcggt gctttgctca aagccaagct ggataacaag aattctgcgc
     3301 aataggaagc gccatgacca agtcggagct gatagccaag ctggcggcac atttccccca
     3361 gctggtcgca agcgatgccg agcttgcggt gaaaatgatc ctggacgcga tggccaaaag
     3421 cctgtcccag gggcatcgaa tcgagatccg gggcttcggg agtttcgggc tgaactaccg
     3481 cccgcctcgc gtggggcgca atccaaagtc tggcgataaa gtacatgttc cggccaagta
     3541 tgtgccacat ttcaaggcgg gcaaggaact gcgcgaacgc gtggacttca agagccagta
     3601 aggcaggcgg cattgtgctt cacggccaaa ccagacaggc ggcatatcgc gagatcgtgc
     3661 cgcttttttt tgcctgatct cgatgcacca ttccccttgg gatgattttg cgcatgcgct
     3721 atctttcgtg ggcttttcgg ctgctgttat tcattctgtt attgggtttt gcgctgaaga
     3781 attccgatcc ggtgagcgtg cgcttctatc tgggcgcccg gtgggatgca ccgctggcgc
     3841 tcgttgcgtt cgtgtttttc gggatgggcg cagcagccgg tgtggcggcg tgcttcattt
     3901 atatttaccg cttgcgcggc gaaatcctgc agttgcgcaa ggaactgcgt gcgaagatag
     3961 ccgcgcccga agacgcgctt tgaatcgggt tgcggctggc acgcattcag tatccggacc
     4021 gtgccatgga atttgagttt tggtggcttc tcggatttcc attgtttttc gccttcggct
     4081 ggcttgctgc gcgcattgac atcaagcagt tggtctccga atcccgctcc ctgccgctgt
     4141 cctacttcaa gggcctcaat ttcctgctca atgagcagcc ggacaaggcc attgaggcct
     4201 tcatcgagct ggtaaaagta gacccgcaga ccattgaact gcatttcgcg ctagggagcc
     4261 tgtttcgccg tcgtggcgaa gtcgaacgcg cgatccgcat gcacgaaaac ctgctggaac
     4321 gggcggatct ggcacccgaa cagaagatga ccgccttgta cgaacttgcg caggattatc
     4381 tcaaggcagg attgctggac cgggcagaag aattgtttgc caaactaaaa ggaacgctat
     4441 acgaacagca gggactgaaa tttttgcttg agattttcga gcaggagaaa gactggagca
     4501 aggccattga aacgtcgcgc aagatggatt ccgtttcggg acagtccagc gcgaaagaca
     4561 ttgcgcattt ctactgcgaa ctggccgccg tggagatcac gcattcgcgt tgggatcagg
     4621 cgcgcgatta tctcagcgaa gcattgagtg taaaccgccg ctgtgcgcgc gcaaacatcc
     4681 tgctgggcga tacggaaatg ggcgaagggc gtcccgccgc cgcgatcgaa tattggaaag
     4741 cgctcgagag ccagaatccg gcctatattg ccttggtggc tgaaaaaata ttggcggcat
     4801 atcgcagcct cggtcgcgat gaggaaggtt tgcaactgct gcgcggctac ctgaccaatt
     4861 atccatcgct tgatttactc aatgtgatct tccagggcga actggaccat ctcggaccgg
     4921 agcatgctta ccgcctggtg cgcaatgaac tggcccgcaa tcccactctc ctgggcctgg
     4981 acaagttgct ggaagcacag ttgctggaag cgccggtcga gcggcgtcac gaactgcagc
     5041 taatcaaaaa cctcgtgcac cagcataccc gcagtcttgc gctgtatcgc tgcgaaagtt
     5101 gcggattcaa ggcacgccag ttctattggc attgtccagc gtgcaaccgc tgggagacct
     5161 atccgccgcg gcgaacggaa gaaagggaat tcggcgcatg aattcgcgga ttattgttgc
     5221 gcttgactat gccgacgcca ggtccgcgac ggcactggtc gatcgtctga atccggcagc
     5281 ctgcaaactg aaaatcggca aagagctgtt taccgccgca ggaccgtcgt ttgtggccat
     5341 gatcgcgcag cgtgggttcg agatatttct cgacctcaaa tttcacgata tcccgaatac
     5401 cgtggcacaa gcctgcaagg cagccgcgca attgggggtc tggatgatca atgtccacgc
     5461 cctcggtggc agggcgatga tgaatgccgc tcgcgaaggc atcgagtctg cagcacacaa
     5521 acccaaggtg attgcggtca ccgtgctgac cagcatggcg gaaaccgatc tgcgcgaggt
     5581 ggggatcgac tcgaatccca cggcggaagt catgaggctc gccgaactgg caagcgactg
     5641 tgggctggac ggcgtggtat gttcggcttg ggaagcatcg gccattcgag accagctggg
     5701 cagtcgtttt ttgcgcgtta ctccggggat ccgcttgccc gaagaagcgg cagacgacca
     5761 aaagcgcatc atgacgccgc aactcgccat tcgctcaggc tccagttatc tggtcgttgg
     5821 ccgatccgtt acccgttcca ccgatccact tgcggtactc gcgcgaataa atggcgagat
     5881 ttcggtaccg gaggtacaac agtgaaaatt acggtcatcg gcaccggcta cgttggtttg
     5941 gtatcgggcg cctgttttgc cgatctcggc aatgatgtgg tttgccttga cgttgacgat
     6001 aaaaagatcg ctcaacttaa aagcggtggc attccgattt atgagccagg actgcagcaa
     6061 ctggtcaatc acaatgtggc ggcagggcgc ttgcgtttta ccacggacgt tgaagaaagc
     6121 gtgacccatg gggccgtgca gttcattgcg gtaggcactc cgtccgatca ggacggttct
     6181 gccgatatgc gctatgtcct ggcggctgcg cgcggcatcg ggcgtaccat gaccgaatat
     6241 cgcgtcattg caaacaaatc gacggttccc gttggcacgt cagagcgagt gcgttccgca
     6301 attgccgagg aactcaaaaa acgcagcctg gacctgccgt ttagcgtggt atcgaatcct
     6361 gagtttctca aggagggcgc cgcagttggc gacttcatgc ggccggaccg gatcgtcgtc
     6421 ggcagttcgg atgaacgcgc aacccagttg atgcgccagc tctacacccc gattctgcgc
     6481 aaccatgacc gcatcttgat catggatccc cgttctgcgg agttgaccaa atacgccgcg
     6541 aacgcgatgc tggccacgcg catctcgttc atgaatgaac tggccaacct ggctgaaaaa
     6601 ctgggggcca atattgaatc ggtgcgtcat ggcatcggtt ccgatcagcg catcgggtat
     6661 cactttctgt acgccggagt cggttacggt ggttcctgtt tccccaaaga catccaggcg
     6721 ctgcagcaca cggccgggga atatggagtg cagttgacga tcgtcgattc ggttgaaaaa
     6781 gccaaccgca cccaaaagca gcgcattctc gagaaggtgc gcagtcgctt cggagaacag
     6841 ttggatgggc gccggttcgc gatctgggga ttatcgttca agccgaatac cgatgacatg
     6901 cgggaggcgc ccagccgcgt aatcatcaat ggactgcttg cgcgaggagc cagcatcacc
     6961 gcttacgatc cggtcgcgat ggaagaggca aaaaagattt tcgcaaaaga atctggtgtt
     7021 cgttttgcaa gctcgacggt cggcgcactc gacggcgccg atgcactgat tatcgtcacg
     7081 gaatggaagg agttccgcag ccccgatttc gaggacatga aatatcgcct gaagtcgccg
     7141 gtggtgtttg acggacgcaa cctttacgat ccggccatga tgcgcgacgc gggaatcgag
     7201 tatttttccg taggccggcc gcagtgatcg gccgcgaagc cgtcaagagg gcgcgagtgc
     7261 tcgtcgtcgg cgacgtcatg ctggaccgtt actggtttgg cgaggtggag cgcatctcgc
     7321 cggaagcgcc ggtgccggtc gtgaaggtgg ggcgcaccga ggaaagactt ggtggtgcgg
     7381 cgaatgtagc gcgcaatatc gttgccgtag gcgctgctgc aggactgtta tccgtagtag
     7441 gcgaagatga agcagcagct tgcatccagt cgctcgttaa agcagacggt atcgatgccc
     7501 agttgcaccg ggatccagcg ctgcatacga ccatcaagtt gcgggttatc gggcggcagc
     7561 agcaactgtt gcgtatcgat tttgagaccg aaccaggaca tgaagtgctc atgtccaagc
     7621 tcgctgactt tgaagcccgg ctgaagaatg ccgacctggt gatcctgtcg gattatggca
     7681 aaggcggact caagcacatc gagcgcatga tttccgcggc acgtgcggcc ggaaaggcag
     7741 tcctcgtcga cccgaagggc gacgattatt cacggtatcg gggcgcaaca ttgcttacgc
     7801 caaaccggtc tgaattccgt gaagtcgccg ggcgctggaa aaatgaagaa gacatgaccg
     7861 aacgcgccaa caagctacgt gcggaactgg gtttggatgc gttactgatt acccgaagcg
     7921 aggaaggcat gacgctgtac cggcaagggg agcgtctgca tgttcccgca gtagcgcgtg
     7981 aagtgtacga tgtcagcggt gcgggcgata cggtgattgc cgttctgggt gtcatgctgg
     8041 cagcgggtgc aggttttgcc gacgctgttt ctttcgccaa caaggcagca ggaattgtgg
     8101 tcggcaaact gggcaccgca gtcgttcatc ccgaagaact tttccactga agcgcaaaga
     8161 tgtatatcgt cgttaccggc gctgccggct tcatcggttc caaccttgtc aaggcgctga
     8221 atgcgcgcgg ggaaacgaac attctggcgg tcgacaatct aagccgcgcc gagaaatttc
     8281 gtaatctggt ggactgcgag atagcggatt atcttgataa agaacatttc cgggccgcgc
     8341 tggatgcagg ctcgttcgat ggcgaaatcg ttgctgtcct gcaccagggt gcctgctcgg
     8401 atacgatgca gaccgatggg cgctacatga tggacaataa tttccattat tcctccgact
     8461 tgctggcgta ttgccagtcg gaatccgtgc cgctgattta cgcgtcgtcc gcatcggtgt
     8521 acggcgccgg tccaatattc aaggaaggtc gcgccaatga aaggccgctg aatatctatg
     8581 gatactcaaa attcctgttc gatcagctgg tgcggcggcg ctgggaggac aatagtgcgc
     8641 aggtggtggg attgcggtat ttcaacgtct atggtccacg cgaaacgcac aaaggcagga
     8701 tggcgtccgt tgccatgcat tgcttcgatc aatttcgcga aggcggcaaa gtaaagcttt
     8761 tcgaaggcag cggaggatac gcagctggcg agcagcgccg cgatttcata tcggtggatg
     8821 atgtcgtcaa cgtcaatctt catttcctgg atcatcccgc acagtccggc atattcaact
     8881 gcggaacagg cgcatcgcag acgttcaacg atgtggccgc tgccactatc aatgctttgc
     8941 gcgttgccga aggaaagaac gagatggcga ttgctgaact gcaagcgcaa ggcctcgtgg
     9001 aatatgggcc ttttcctccg gcgctcaagg acaagtatca aagcttcacg gaagccaacc
     9061 tcagtgctct ccgtggtgca ggctatgcag cgccgtttgc ggatgtgcgc acgggagttg
     9121 ggcgttatgt ccaatggatg ctgaagaacc gctgatcgtt tcgctggaga tgggaacaca
     9181 ggccaatttg cgcagagacc gcgatcgcgc cggcagcggc aagacgcttg atgatttcgt
     9241 cggcaatact cccttggtgc gcttgaagcg gattccggga aacaccacta atgtaatcct
     9301 cgccaaactt gaaggtaaca acccggctgg ctcggtaaag gatagacctg cgctatcgat
     9361 gatccgtaaa gcggaagagc gcggcaggat aaaggctggt gatacgttga tcgaagcaac
     9421 cagcggcaat actggcattg cgcttgcgat ggtggccgcg atgcgcggct atcgcatgat
     9481 tctcgtcatg ccggagcacc tttcgctcga acgccggcag agcatggcgg cattcggcgc
     9541 caaattcgtg ctgacgccca aacaaggcgg gatggaaaaa gcgcgcgacg tggcggagca
     9601 gatgcaggcc gctggcgcgg gtctgattct tgatcagttt gccaatcccg acaacccgct
     9661 ggcccattac gagggaaccg ggccggaaat ctggcgggac actgacgggc agatcacgca
     9721 cttcgtcagc agcatgggca ctaccggtac catcatggga tgcagtcgct atttcaagga
     9781 gaaaaatccg gccatcgcta tcatcggatg ccaaccaact gaaggctccc aaatacccgg
     9841 catccgaaaa tggcctcacg cctatatgcc gcggatcttc gagcctagtc gcgttgatcg
     9901 agtggttgaa atagcacagg aagatgccga agacatgacg cgccggatgg cccgggaaga
     9961 aggtattttc gccggggttt cttccggggg tgcgctgtgg gctgcactgc agatttcagc
    10021 tgaaacgcga aatgcaatta tcgtcaccat cgtgtgcgat cgcggtgacc gttatctttc
    10081 aaccggcatt tttcccgccg aatagcaccc gaaatcgaac ggcgctaagt ccagtattgg
    10141 gtcgcgtgct ttagactgga caacagcgat tcccgcttga gcgcaaactc ttgcggcaag
    10201 cgttcgctta attttgcgaa cagttcatca tgcagcgcga gttcacgcgt ccacccgtcc
    10261 ttattcaggc gcatcaattc cgtgaagcgc tccgtcggga attcaagacc gctccattcc
    10321 agatcatcct gccgcggcat ccatccgagg ggactttcct gcgcgcctgc tttttcctca
    10381 caacgatcca caatccattt cagtacccgc atgttctccc cgtacccggg ccacaagaat
    10441 ttccccttcg cgtccttgcg aaaccagttc acgcagaaga tgcttggtgg ccgttcgata
    10501 ctgcgcccca ggcggagcca gtgcgagaaa taatcaccca tgtgatagcc acaaaacgga
    10561 agcatcgcca ttgggtctcg ccgcacttcg ccgacttttg ccgtggcagc agcggtcgtc
    10621 tcggaaccca tcgtcgcagc catgtagatg ccgtcggtcc agttgaatgc ctgcatcacg
    10681 agcggaacag tgttgctgcg ccggccgccg aaaatgaagg ccgctattgg aactccttcg
    10741 gggttttccc aatctggatc caatgaagga cattgtgcgg cgggaactgt aaagcgcgcg
    10801 ttgggatgcg ccgccggccg cccagaacct ggcgtccagg gttttccttg ccaatcggta
    10861 aggtagtcgg gaggctcgtc tgtcatccct tcccaccaca cgtcaccgtc cggcgtcagt
    10921 gctacattgg tgaaaataca attggcacgc agcatggcca ttgcattggg attcgatttt
    10981 tcagacgtgc cgggggcgac accgaagaag ccgtattccg gattgatggc gcgtagccgg
    11041 ccttcggcat cgggtttgat ccaggcaatg tcatcgccaa ccgtcgtgat cttccagcct
    11101 tcaaacgcct tgggcggaat catcatcgca aaattggtct tgccgcacgc gctcggaaaa
    11161 gccgcagcga cataggtctt gctgccgcgt ggcgactcga cgcccaaaat taacatgtgc
    11221 tcggcgagcc aaccctctcg ccgtccaatg ttcgatgcaa tgcgcagtgc caggcatttt
    11281 ttccccaaca aagcattgcc gccgtagccg ctgccgaacg accaaatggc attttcttcg
    11341 gggaaatgca cgatgtactt gtggtcactg ttgcacggcc atgcgacgtc ggcctgcccg
    11401 tgggcaagcg gcattccaac agagtgcagg cacggcacaa attctccatc ggcgcccagt
    11461 gcatcaatga cctgtcgtcc catacgcgtc atgatgcgca tgctgaccgc cacgtaggcc
    11521 gaatccgtta tctgtacgcc gatcagagaa agcggagaac caatcgggcc catcgaaaag
    11581 ggcacgacat acatggtacg accgcgcatg ctgccctcga acagcgactg caaagtgcgc
    11641 ttcatttttc gtggctcggt ccaattgttc gttggaccgg cgtcctgctc gcgctcactg
    11701 cagataaacg tacggtcctc cacgcgcgcc acatcagaag gatcggacag tgccagatag
    11761 ctgccgggac gcttcagcgc attgagccga cgtaacgtgc ccgccgcaac caggcgcgaa
    11821 catagcattt cgtattcgcc ctgtgatccc tcgcaccaat ggatattttg tggttgggtc
    11881 aatcgggcta cttccgcgac ccaatccagc aagcctgcgt tctgcacgaa gtctggatgg
    11941 ccaggaaggg cagcctgatt tcctctgctc aggctggcga tcgttttcac aagtaattct
    12001 ccccaaagct atgattggtt ttgttgcgag tctaacatcg gacatcgggc gcagggtcgc
    12061 gttcactgat cggccctgcc tcagcgcctt gttgcgccgc ataatattca cagttcaatg
    12121 gcgtcagcga tgcaccacaa agttcaggaa tgtaccggtc atttccggta ttctctcgcg
    12181 cttccgcgct gccgaaaaat cgcgcggcca gaaaattctt ttacggtatg acaccaattc
    12241 ttgcattcga tattgagacg gttccggatg tgtccgggct ccgcaagcta tatggtcatg
    12301 cgccagacac acccgatggc gaagtggcgc agattgcgtt tgatcgcaaa cgagaagcga
    12361 ccggcagcga ttttctgccg cttcatctgc atcgcgtggt ggctatttcc tgcgcgctac
    12421 gtgaccggga tagtttccgg gtctggtccc tcggtgagtc cggtgagtcc gaggcggaat
    12481 tattgcagcg atttttcgac gggatcgaaa aatacacacc gcagctggtc tcctggaatg
    12541 gcagcggatt cgatttgccc gtattgaatt accgggcact gatccatggc gtgcacgcgg
    12601 cccgcttttg ggacctcggc gaggaagatc gcgacttcag atggaacaat tatatcggcc
    12661 gctatcacag ccgccatctg gatttgatgg atgttcttgc gatgtaccag ggcaggggca
    12721 acgcaccgct cgaccagatg gcccagctgc tcgggcttcc agggaagctc ggcctggatg
    12781 gatccaaggt ttgggatgca ttccagcaag gccatattgg agcgatccga agctactgtg
    12841 agacagacgt cgcgaatacg tatttgctct atctgcgttt tcaattggtg cgagggatta
    12901 cggacacggt agcttatcgt cgagagtgcg accttgtgcg cgcaacgctt ttgaaatccg
    12961 gggaaattca ctggcgcgag ttccttgccg gctgggcgga tgagggctga gccgatcgcg
    13021 cgttttgcgc aggtggagtc gctcgaccag gaaggtcgtg gcattgccca tgtcgaaggc
    13081 aaagccatct tcatcacggg cgcgctcccc ggtgaatcgg tcgaatatgt gccataccag
    13141 cgcaagccga actacgaact ggccgacacg acccggatac tgcgcgcatc gccgtcacgc
    13201 gtaattccgc gttgcccgca tttcggcgtg tgtggcggct gtagtctgca acacatggac
    13261 ttgcgggcgc aggtggccgc gaagcagcga atcctcgagg acaatctggc tcgaatcggg
    13321 aaggttcgtg cacagatcat gctgtcacca attctcggtc cggattggcg ctaccgctac
    13381 cgggcacggc tgtctgcgcg ctatgtcgaa aagaaatcca ggcaattgct tggctttcac
    13441 gagcgccgca gcagtttcgt cgccgacatg gggtcctgcg aagttctgcc ggcaagaata
    13501 tcagccttgc tggaacctct cagggaactg attggctcgc tgtccattcg cgagcgagtc
    13561 ccccaggtcg aactcgccat cggggatgca gtggatgttc tggtcctgcg ggtcctggaa
    13621 gctccccgat ccgacgatgc cgagaaactg cgtagctttg cggagcaata caagatccag
    13681 ttctttctgc agcccggcgg cccggataca gcacgcgcgt attacccgca agacgcggcg
    13741 gtgttgcagt accgtattcc ggattttgat ctcacttttc cgtttcatcc cactgacttc
    13801 acccaggtaa accatgacat taatcgggta ttggtacggc gcgcactgat gttgctggat
    13861 ccgcaaccgg gagaacgtat tggcgatttc ttttgcggac tcggtaactt ttcgctggcc
    13921 attgccagac ggggtgcgca ggtcctgggc atcgacggca atgcgaggct cgcgctgcgc
    13981 gcgaaggaaa ctgctttact gaacggcgtc gctgaccaat gcagcttcat cgatcgagac
    14041 ctgtttaaaa tcacggaaga cgaatggaag aaaatagctc ccttcgatcg gctactgctt
    14101 gatccgccgc gcgatggggc aatggaactg gtgaaggcat tgggacaaat ggcacccgga
    14161 cgagtggtgt atgtctcatg taatcccgca acactcgcgc gagacgcagg tgttctcgtg
    14221 aacgtgcacg ggtatcagct atccgcagcc ggtgtcatca acatgttccc gcatacttcg
    14281 cacgtcgaat caattgccgt atttgaccgt tagtcgcttt cgggtgaaaa aaaagcggcc
    14341 ttgcggccgc ctttgatgcc gtgcaatccg atcagtcgcg ctgtccggta aacgccatga
    14401 gcaactgcag cagattgaca aagatgttgt acaggctgat gtagaccccg gtggccgcca
    14461 tgatgtagtt cgtttcgccg ccggtaacga tgcggcttac atcgtgcagc aggaacaggg
    14521 aaaagacgac gaccactaat gcagagatgg tcagcgccag ggccggaatc tggaagaaca
    14581 tgttcgccaa catggcgagc aacagcacga tcatgcccac gaacaaaaat ttgcccataa
    14641 agctgaagtc ttttttcgtg gtggtcgcaa tgaggctcat ggctaccaga atgcctccgg
    14701 taccgattgc cgcgattccg atcaactgcg gaccattctg caatcgcaat gcaaagttga
    14761 gcaaaggccc cagccaccag cctagaacga acgtcatccc gagcaacaag gccacaccca
    14821 aaccgctgtt gcggttggcg gcaatggcaa actgcaaacc tatgatcgcg ccgagcatca
    14881 gcagcgtcgc catgatggga tgttgcagga tgattccgct cgttgacagg ccgacgaagg
    14941 cgccgataac agtcggcacc atggttaggg cgagcagcag gtacgtattg cgcaatacct
    15001 tgttctgctg gacggacagc gcccccggct gagaaccata ttggatttcg ggttgcatat
    15061 cgtttactgc ctcctcagaa gaattgcttt cgtaagacta cgcagttgct gtaaaagttt
    15121 caatggcttg cgtgacggct caatgccaaa gcaggtacta tcctacagtg atccggtacg
    15181 cggcgctggc cggaatggag ctgggggccg gccggctgga atgcaatggc atcatcgctg
    15241 aaaagtcgca agctggcgcg tttcaattga cggccctttc gagtgtcccg ctaagcagag
    15301 tatcgtatcc ctatgcggag gtggcagagc ggttaaatgc accggtcttg ggcacgcgaa
    15361 gcttgcgcta gcgcagggta acctttcagg ttagccggag ctaaaaccgg cagcagcaat
    15421 gtatcgcagc atggaagggt ggcagagcgg ttaaatgcac cggtcttgaa aaccggcaga
    15481 ggcgcaagcc tctcgtgggt tcgaatccca ccccttccgc catcagggat acggggtcag
    15541 tccacaatgt cccggctggc attcagagta caatcagcgg ccccggtact gtgatatgcc
    15601 gtgtctatct cctggctggg gaaataggcg gcccgacaag caagatgcag gcgtgcagca
    15661 atagcatcac agggcgaaaa agacctgaat tcgaaacacg ctag
//
LOCUS       DATMZQ010000295         2723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_588329, whole genome shotgun sequence.
ACCESSION   DATMZQ010000295 DATMZQ010000000
VERSION     DATMZQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588329"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VN496_09635"
     CDS             complement(<1..248)
                     /locus_tag="VN496_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXF17254.1"
                     /translation="MVTTTLKSGFGRAKQRSARAHATPVTAVVKGQAKATAFRMAGVQ
                     MASGPNVAANLNEAGRLIEIAAKQGARLIALPEYFAIMG"
     gene            complement(315..>2723)
                     /locus_tag="VN496_09640"
     CDS             complement(315..>2723)
                     /locus_tag="VN496_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXF17255.1"
                     /translation="FLTADVSWVVKRDGIRLELKNASFTNEDLAGTVSGSYFRTADGR
                     GSIDLNGALLRAEVRQVWRYVPTFIAGPQAWLKRALLAGDARETRFRLKGALNDFPFK
                     DDKKGIFEVKTKASGVTMDFADGWPPITGVSGEVLFHGARMDIKGQSGAILGLHLSDV
                     QVSHAELGKRDERLVIKGTVRGPTSEFLRFAATTPVAGYVNHATDDMKATGDARLDLE
                     LGLDFGDSKMSTVKGNLLVENNQVSIPRLPSLERAAAHISFTRSDLKVSDGRALMFGS
                     PVSFELSSQPDGGINSSISGTLEMSRARELWKHPALALFDGQAAWRGTITVRNKMVVG
                     HLDSNLVGLASSLPAPFGKKAATSLPLHLELREQAGRRRTLEGNLDKVASAVLLLDET
                     APGGVSRGMLSIGSVATVPASEGIWIKGRLESVDADVWHDLMSGGLGRSTSRPDLAGV
                     DLDIGVLDFNRRRFHDVKIYATPNDEEWRIKLGGREVVGDLYWTSDGDGKLTARLSKL
                     VLPPVASAIEPSKPAGTDPSLPSVDVIAENFNYEGKDLGRLLVLAQPEFSGWQLRRLE
                     VTNPDSKFVMSGHWSLGSMSHTDVSIKLDVSDIGKFFTRLGWPNAVKGGSATLEGPIK
                     WSGNPTRFDIPSLSGQLKLAAKDGRFQEIEPGVAKLLGILSLQALPKRVNLDFRDVFS
                     KGFSFEHIDADVNIVNGVANTQNFVMQGSAARVAMHGQVDLGRETQKLSVRVTPALSS
                     GIAIAGAIVNPALGIAALIAQKALKDPFSKLASFDYTISGTWGDPVVARVVRVIPQVE
                     KEGR"
BASE COUNT          549 a          854 c          743 g          577 t
ORIGIN      
        1 cccatgatgg caaagtattc tggcagcgca atcagccggg caccctgctt cgcggctatc
       61 tcgatgagcc ggccggcttc gtttaagttg gcggcgacgt tagggcccga ggccatttgc
      121 acaccggcca tgcggaatgc cgttgccttg gcctgtcctt ttacgaccgc tgtgaccggc
      181 gtggcgtggg cgcgcgcgct acgttgcttt gcgcgcccga agcctgattt cagggtggta
      241 gtgaccatgc agttgcgaag ccttgcgtgt acctttagcg agcatggatt gtaaccctcg
      301 ccgccgacga acctctagcg accttccttt tccacctgcg gaatgacgcg cacgacgcga
      361 gcaacgaccg ggtcgcccca ggttccgctg atcgtatagt cgaaagaggc caatttgctg
      421 aatggatcct tcagggcctt ctgtgcaatc agtgcagcga tgccaagggc cgggttgacg
      481 attgcgcccg caatggcaat gccgctcgac agcgccggag tgactcgcac cgaaagcttc
      541 tgcgtttcgc gcccaagatc cacttgcccg tgcatggcca cgcgtgcggc ggaaccctgc
      601 atgacgaagt tctgcgtgtt ggcgacaccg ttgacaatat tgacgtcagc gtcgatgtgc
      661 tcaaagctga atcccttgct gaagacatcc cggaaatcaa ggttcaccct tttcggtaag
      721 gcttgcaggc tgagaatgcc cagcaacttc gcgacgccgg gctcaatttc ctggaaacga
      781 ccgtctttcg ctgcgagttt aagctgtccc gatagcgagg gaatgtcgaa ccgggtcggg
      841 ttgccgctcc acttgattgg gccttcgagc gtggcactgc ctcctttgac ggcattcggc
      901 caacccagtc gcgtaaagaa cttgccaatg tccgaaacat ccagcttgat gctgacgtcg
      961 gtgtgcgaca tgcttccaag tgaccaatgg ccgctcatga cgaacttgct atccgggttg
     1021 gtaacttcca gccggcgcaa ctgccaaccg gaaaattccg gctgcgccag caccagcaag
     1081 cgcccgaggt ccttaccttc gtaattaaag ttttcggcga tcacatcgac cgacggcagg
     1141 cttggatcag tcccggccgg tttgctgggt tcaatcgcag aagcgactgg gggcagcacc
     1201 agctttgaca gacgcgcagt cagcttgcca tccccgtcgg aagtccaata gagatcgccc
     1261 accacttcac gtccgccgag ctttattcgc cattcctcgt cattcggcgt cgcatatatt
     1321 ttgacatcat ggaaacgccg ccgattgaaa tccaggacac caatgtccag atcgacgccg
     1381 gcaagatcgg gcctggacgt ggaccggcct aatccgccgg acatcaaatc gtgccagaca
     1441 tccgcatcca ccgattcaag ccttcctttg atccagatac cttcggaggc cggtactgtc
     1501 gccacgctgc cgatgctcaa catgccacgg ctcacgccac cgggtgccgt ttcgtcgagc
     1561 agcaatacgg cggacgcgac tttgtccaga ttcccttcga gcgtcctgcg gcgtcctgcc
     1621 tgctcgcgca attccaggtg caacggcaga ctcgtcgcgg cctttttgcc gaaaggcgcg
     1681 ggcaacgacg aggccagacc aaccaagttc gaatccaggt ggccgacgac cattttgttg
     1741 cgcactgtga tcgtgccgcg ccaggctgcc tggccgtcga acagcgcaag cgcgggatgt
     1801 ttccacaact cgcgcgcccg actcatctcc agcgttccgg aaatgctgga attgatgcca
     1861 ccatccggtt gactagacag ttcgaaagaa accggtgagc cgaacatcaa tgcgcgtccg
     1921 tcgctgacct tcaaatcgct tctggtgaag ctaatgtgag cagccgcacg ttccagcgaa
     1981 ggtagccgcg gaatgcttac ctggttattt tcaacgagca agttgccttt gaccgtggac
     2041 attttgctgt ccccaaaatc cagacccaat tccagatcaa gtcgcgcgtc tcccgtggcc
     2101 ttcatgtcat ccgtggcgtg attcacgtag cctgccaccg gcgtagtcgc cgcgaaacgc
     2161 aggaattccg acgtggggcc gcggaccgtg ccttttatca ccagacgttc gtcgcgcttg
     2221 ccaagttcgg catgcgagac ctgtacgtcg gacaggtgca aaccgagaat cgcgccagac
     2281 tgacccttta tgtccatgcg cgcaccatgg aagaggactt caccgctcac gccggtaatg
     2341 ggcggccagc catcggcgaa atccatggtg actccgcttg ccttcgtttt cacttcaaag
     2401 atgcccttct tgtcatcttt gaacggaaaa tcgttcagcg cgcctttgag gcgaaagcgc
     2461 gtttcccggg cgtcgcccgc cagcagcgca cgcttgagcc acgcttgcgg cccggcaata
     2521 aacgttggaa cgtaccgcca tacctgtcgc acttctgctc gcagcaatgc gccgttcaaa
     2581 tcaatgctgc cacgtccgtc tgctgttctg aaatacgaac ctgaaaccgt acccgcgaga
     2641 tcctcattcg tgaagctcgc gttcttgagc tccaaccgga tgccatcacg ctttactacc
     2701 cacgacacgt cagccgtcag gaa
//
LOCUS       DATMZQ010000296         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1969890, whole genome shotgun sequence.
ACCESSION   DATMZQ010000296 DATMZQ010000000
VERSION     DATMZQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969890"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VN496_09645"
     CDS             complement(<1..1004)
                     /locus_tag="VN496_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXF17256.1"
                     /translation="MARNRLGHEEVEPRQSVCIRTGRLIALVRIAGLCTGLLSCLPAQ
                     PQSPESWYVPPPHKFDPGPPPEPDEPFAVPDVPQAVQEAAIVTPWLTVSPGLVLIGDY
                     TAFSQDANSLAQVGRQENQAEFRAFRLVLLGRMGTDYQVSYLLAAEYNGLDRDPNQNW
                     ELMDLAFTFPIGSPATKLTVGKTKETFAYEMVGDSANLPQAERVLNPFFVSRNIGAKV
                     THVIGEDQRMTVSAGAFNDWWAKGDDYGDAGTDVTGRVTGLVWDENQGTRFLHLGLSG
                     RYVGADNNTLRYRARPESNVSSYYVDTGELPADHAWHVGLEALWNQGPYSVLAEYNRA
                     "
     gene            1305..2387
                     /locus_tag="VN496_09650"
     CDS             1305..2387
                     /locus_tag="VN496_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17257.1"
                     /translation="MKGIVCLALALPGLLWLAACASKPLVAFSTDTPPLILTPTALAG
                     SEDKRARFREIYCAVLERDTNQFPDYRRCDDALTRVGTEPAATGRAVDVDASKRHLIA
                     VVVPGIGYDCIAPWLASAGTVAAHLRKQGYDLMSIKVDALSSTARNARQIRDAIMAMP
                     AEEGGPRLVLMGYSKGTPDILQALVTYPEILSRVAAVVSAAGAVGGSALANDAEQYQA
                     NLFRYFPGATCDAGDGGGVAELRTDVRKAWLAQHRLPAGPNYYSLVTFPHPQRISSIL
                     EGSYDKLSRIDARNDGQVIFYDQIIPGSALMGYVNADHWALAVPIARKHSTISSLFVT
                     QNAYPREALTEAILRFVEDDLGGSAQ"
     gene            2441..>3775
                     /locus_tag="VN496_09655"
     CDS             2441..>3775
                     /locus_tag="VN496_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17258.1"
                     /translation="MNRARRILLPEVLAFVWLMHVAQPVWAWIYPEHRDIALLAVETL
                     DPERKVIFERLWNDARTGSEQRLCPAGGDASQGLAPVCIDWAAFSGIAGDHSCSSRNM
                     LDIALKSDWILKVATVAAQLKIDLAQIETSVPAGEREKSTNLIVDLQRQLEAEAVRAR
                     RINALRTSDNQLQSADPEYATRAGASNAHFLLPRPRTDVSPAEYARITLSPGSEISAI
                     GVYTWYHFSALQKATRLANEQLAPEQRQALGRAMLADEAFALHFLEDIFAAGHVAGSW
                     GDASARKGTHDYYNEAGLEVFLWKGSNQSMVLMGDAHMRPQDAERAAVAVRTSLEQVL
                     DHAAGRERTVNLAHTPGAPAAPDTFDVCKNHSLVRREEGQRVTPESFELAYEVLRPTP
                     VPGLGPGLGALPRFRAELGPFVGVAGSMDLRNISGGFAESQDENGWMGGVDLS"
BASE COUNT          747 a         1113 c         1167 g          748 t
ORIGIN      
        1 caggcgcggt tgtattcggc gagcacggag tagggccctt ggttccagag tgcttccagt
       61 ccaacgtgcc acgcgtggtc cgcgggcagc tcgcccgtat caacgtaata gctcgatacg
      121 ttggactccg gccgggcccg ataacgcagc gtgttgttgt ccgcccctac ataacggccc
      181 gaaagaccga gatgcaggaa gcgcgtgccc tgattttcat cccataccag tccggtcacg
      241 cgcccggtta catcggtacc ggcgtccccg taatcatcgc ctttggccca ccaatcgttg
      301 aaggcgccgg ccgaaacggt catgcgctga tcctctccaa tgacgtgcgt tacttttgct
      361 ccgatattgc gcgagacgaa gaaaggattc agcacgcgtt cggcctgcgg aagattcgct
      421 gaatcgccga ccatctcgta agcgaaagtc tccttcgttt taccaacggt aagcttggtt
      481 gccgggcttc cgatcgggaa agtgaaggcc aaatccatga gttcccagtt ttgattcgga
      541 tcgcgatcga gcccgttgta ttccgcggcc agcaggtagc tgacctggta atccgtcccc
      601 atgcgcccca gcaagaccag acgaaaagcg cggaactcgg cctgattctc ctgccgtccg
      661 acttgcgcca ggctattggc gtcctgcgag aatgcggtgt agtcgccgat caggaccagt
      721 cccgggctga cggtgagcca gggcgtcacg attgccgctt cttgcacggc ttgcggcaca
      781 tcgggcacgg caaagggctc gtccggttcc ggcggtgggc ctgggtcgaa cttgtgcggc
      841 ggcgggacgt accacgattc cggggattgc ggctgtgcag gcaggcagga cagcaaaccc
      901 gtgcacaggc ccgcaatgcg caccagagcg atcagtcttc cggtgcgaat gcacacgctc
      961 tggcgtggtt caacctcttc atgacctagt ctgttgcggg ccattcgctc ggccattgtt
     1021 gttatctccc ggcttcttcg cttgaactgg ctacctcagt ttgcatgata aacgtcacct
     1081 gttggcgatg aagtgattga tatggattaa acgaggcacg ctaggggata ggtaacacgt
     1141 cgcctttttt gcacgttcag gtagactgga acgcaagctg tccgcccggg caccgcggga
     1201 atgatgatgg ctgaatctaa ggggcatcga ataatgcggg agaagccggt cgttgcaggg
     1261 ggccgtgcgc atgccgtcgc gtacgctcgg cgagagagcg cgttgtgaaa ggcatcgttt
     1321 gcctggccct tgccctgccc ggactccttt ggctcgccgc gtgcgcgagc aagccgcttg
     1381 tagctttctc caccgacacg ccgccgctga ttctcacccc aactgccttg gcgggctcag
     1441 aagacaaacg tgcacgcttc cgcgagatct attgcgcggt actggagcgg gacaccaatc
     1501 agtttcccga ctatcggcgc tgtgacgatg cgctgacgcg cgtcgggaca gagcccgctg
     1561 caaccgggcg cgctgtcgat gtcgacgcct cgaagcggca cctcattgcg gtcgttgtgc
     1621 cggggattgg ttatgactgc atagcgccat ggctggcgtc tgcaggcacg gtcgccgcac
     1681 acttgcgcaa gcaaggatac gatctgatgt ccatcaaagt ggacgcgttg tcgagcacgg
     1741 ccaggaatgc cagacagatt cgggatgcaa tcatggccat gcccgcggaa gagggtgggc
     1801 cgcgcctcgt gttgatgggc tactcgaagg gcacgccgga tattctgcag gcacttgtca
     1861 cgtacccgga gatcctcagt cgcgtcgctg cggtggtcag cgcagcaggc gcggtgggcg
     1921 gttctgcact ggccaacgat gccgagcaat atcaagccaa cctgtttcgt tacttccctg
     1981 gtgcgacttg cgatgctggc gatggcggcg gagtggcgga actccggacg gacgtacgca
     2041 aggcctggct cgcccagcat cggttgccag ctggaccaaa ttactactcg ctggtgacat
     2101 ttccccatcc gcaacgcatt tcatcgatcc tcgaggggag ctacgacaag cttagccgca
     2161 tcgatgcgcg caacgatggc caggtgattt tttacgacca aatcatcccg ggctccgcat
     2221 tgatgggtta tgtcaacgcc gaccactggg ctctcgcggt gccgatcgca cgaaaacatt
     2281 ccacgatcag ctcgctgttc gtcacacaaa acgcctatcc gcgcgaggcg ctcaccgaag
     2341 cgattctgcg cttcgtcgaa gatgacctgg gcgggagcgc acagtagaaa ctcgaagcaa
     2401 cccacgaata acgccagtca gccgcaagga gccaaactga atgaatcgag cgagaagaat
     2461 tctgcttccg gaggtgctcg cctttgtctg gctcatgcat gttgcgcagc ccgtctgggc
     2521 atggatttat cctgaacatc gtgacatcgc gctgcttgcc gtagaaacac tcgaccccga
     2581 acgcaaggtg atattcgagc gcttatggaa tgatgcgcga accggcagcg agcagcgttt
     2641 gtgtccggcg ggaggcgacg catcgcaagg cctggcgcca gtgtgcatag actgggccgc
     2701 gttctcgggc atcgccggtg atcactcatg ttcgagcaga aacatgcttg atatcgcact
     2761 caaatctgac tggatcctca aggtggccac cgtcgccgcg cagttgaaga tcgatcttgc
     2821 gcaaatcgag accagcgtgc cggccgggga gagggaaaag agcacaaatc tgatagtcga
     2881 cttgcagcgc caattggaag ccgaagccgt gcgggccaga aggatcaacg cactgagaac
     2941 ctcggataac cagctgcaaa gcgcggatcc cgagtatgcc acccgggcgg gtgccagcaa
     3001 cgcgcacttc ctgctccctc gcccgcgcac tgacgtgtcc ccggcggaat atgccaggat
     3061 tacgttgagt cccggctccg aaatcagtgc cattggcgtg tatacctggt atcacttcag
     3121 cgccttgcaa aaggcaacgc ggcttgctaa tgagcagctt gctcccgaac agcggcaagc
     3181 gctcgggcgc gccatgctgg ctgacgaagc gttcgcgctg cacttcctgg aagacatttt
     3241 cgctgcggga cacgtggccg gttcctgggg tgacgcctca gcgcgcaagg ggactcacga
     3301 ttactacaat gaggccggac tggaggtatt tctctggaag ggcagcaacc aatcaatggt
     3361 gctgatgggt gacgcgcata tgcgcccaca ggacgccgag cgcgccgctg ttgccgtgcg
     3421 taccagcctg gagcaggtgc tcgaccatgc tgcaggtcgc gagcgcaccg tgaatctcgc
     3481 acatacgcca ggcgcgccag ccgcgcctga tacatttgac gtgtgcaaaa accattcgct
     3541 ggtaaggcgc gaagaaggcc agcgtgtcac gccggaatcc ttcgagctgg cttatgaagt
     3601 tttgcgacca acgccggttc cgggtctggg gccggggcta ggcgcgctgc cacgcttccg
     3661 cgccgagctg gggccgttcg tgggcgtggc cggttccatg gatctgcgaa atatcagcgg
     3721 tgggtttgcc gaatcgcaag atgaaaacgg atggatgggg ggcgttgatc tttct
//
LOCUS       DATMZQ010000297        14370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1970329, whole genome shotgun sequence.
ACCESSION   DATMZQ010000297 DATMZQ010000000
VERSION     DATMZQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970329"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VN496_09660"
     CDS             complement(<1..764)
                     /locus_tag="VN496_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF17259.1"
                     /translation="MYILCGPLLHHHDIAMYLTVDNRKTYAYTANRAFDASKPTVAFI
                     HGAAHDHSVWSLQSRFFAYHGWNALAIDLPGHGKSHAPAFTAIEDAAVWIARLLDEAG
                     ATRAALVGHSMGALIALECASRYPSRISRIALIGCGVPMPVSAPLLNGSKANDHAAFD
                     MINVYSHSAAAQMGGNRTPGMWMMGSGMRLLERSAPGVLHADFTACNSYSAGLAAAKS
                     LRCPVLMILGKRDLMTPVTLAKALVSELANVRVVTL"
     gene            869..1276
                     /locus_tag="VN496_09665"
     CDS             869..1276
                     /locus_tag="VN496_09665"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF17260.1"
                     /translation="MFQKRSAMIVSGMVLAFAVGIAVGQNAAPTANKGVNVGAPTFLD
                     LTDEIDSVAGRQLRMRVVSIDPGGVVALHSHNGRPAVAYALQGTLVEHLENGDVREHP
                     QGESWTEDKNTTHWAENKSNQPVKVIAVDVFKP"
     gene            complement(1377..1658)
                     /locus_tag="VN496_09670"
     CDS             complement(1377..1658)
                     /locus_tag="VN496_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17261.1"
                     /translation="MKNAAIGFAMVSALGLAGCKQEQAPGVYVEISLKNDADIDRYVV
                     FNPNIKTLEECKRSFGDSLPSIMANLPQAIPKNSTVTGWKCSLKDPAKK"
     gene            complement(1695..2990)
                     /locus_tag="VN496_09675"
     CDS             complement(1695..2990)
                     /locus_tag="VN496_09675"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HXF17262.1"
                     /translation="MPGPKTYQFDTLSLHAGQQPDPQFGARAQPVYFTTSYVFKDTDH
                     AASLFNMERAGHVYTRISNPTNAVLEERISALEGGVGAIATASGQAALHLSIATLMGA
                     GSHIVASRSLYGGSHNLLQYTLPRFGIETTFVNPRDMDAFRKAIRPDTRLVFGETLGN
                     PGLDVLDIAQVAEIAHEAGLPLLVDGTFTTPYLLRPFDLGADLVFHSATKFLGGHGVA
                     IGGLIVDSGKFDWEKSGKFPTLTEPYAGFHGMVFSEESTTAAFLLRARREGIRDFGAA
                     MSPMNAFQILQGMETLGLRMEKHTANTRAIVQFLSGHDAVESVSYPELASHPDHELAK
                     AILPRGCGAVFTFSLKGGRAAGRRFIEALKIFSHLANVGDAKSLVIHPATTTHYRMDN
                     AALLASGITEGTVRLSIGLENQEDLIEDLSRALRASQKS"
     gene            complement(3015..4142)
                     /locus_tag="VN496_09680"
     CDS             complement(3015..4142)
                     /locus_tag="VN496_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HXF17263.1"
                     /translation="MNLRPAPPARPGMSLAEVDTPALLLDLDAFERNLDRLDKSLAGL
                     RIRVRPHAKSHKCAEIALRQMARGAIGVCCQKISEAEAMVEGGVMNVLVSNEVVGAPK
                     LARLAALARRARISVCVDNEQNVVDMDAAARAAGAKIDVLVEVNVGGNRCGVEPGPAC
                     VDLAQRIAASRQLRFAGLHAYQGAAQHARTPAERKAAIAQAVQYVRITVEALQANGLK
                     PEIITGAGTGTYLLEAASGVYNEIQPGSYIFMDADYNRNLGENGKPTHAFEQSLYVWA
                     TVMSHASPERAVVDAGLKALSVDSGMPLVADLPGVEFLKASDEHGVLKLAQGTHVTVG
                     DKLRLVPGHCDPTVNLYDWIVGLRADKVECVWPVTARGAFY"
     gene            complement(4200..4508)
                     /locus_tag="VN496_09685"
     CDS             complement(4200..4508)
                     /locus_tag="VN496_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17264.1"
                     /translation="MSKRIASGLLIMLSTAAMAAPYPLGTMTCADIGSFASEAMRWRK
                     EGQSRDQALAALEKRSYNDAVEKKNLTVVVDLVFGSYGNNWSVESAGNVLRTDCETGR
                     "
     gene            4597..5280
                     /locus_tag="VN496_09690"
     CDS             4597..5280
                     /locus_tag="VN496_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF17265.1"
                     /translation="MSLEQSEKAVATRCLLAALTFVVICAGVDAAQPGNNETRKQALA
                     QLQDSDAAGRLAGCTVLAEIGRPEDLPLLQARLSDEDYRIRDSAEAAIWAIWSRSGDA
                     AVDELFREGVEQMRSANLKAAAETFSRIISMRPQFTEAWNKRATVYFLLGQDDLSLKD
                     CDEVVKRNPQHFGVLAGYGQIYLRKGDLPRAQEYFERALAVNPNMGGVQASLDAIRKI
                     LIKRGERYI"
     gene            5380..6759
                     /locus_tag="VN496_09695"
     CDS             5380..6759
                     /locus_tag="VN496_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HXF17266.1"
                     /translation="MPDSTARAVLSTLYGGLNLGLFRHPGAARPCVTSTQFIVIAVAS
                     LAVSGVCSFVLSGPGALFNPQALASQLLWVPLALLAGHLVGRLLGDERYLLQVATTVG
                     TIGIALSVASGAVWFAVDRGWIRVPAVLGVSGIYDLLFAWWAMAIVVAVRRMTFSSSH
                     RTLWPVFIVTLMVVVPVYLVTPEPLWEAPGEDTPPETGSRPFGERALYAQTELLRKAE
                     QRLKPERAGVEDLYFVGFAPDASQDVFMKETLAIGRLMDARFGVEGRSIKLISHPGVV
                     ETFPIATLTSLRHALHAIGSRINPEEDVVLLHVTSHGSENHELSVAFFPLELRAIAPD
                     DVRSALDDAGIKWRIVVISACYSGGFIEALQDAHTLVITASDATHTSFGCGNAFDFTY
                     FSKAYFDEALRHNYSFEKAFFIAQKAIAAREKAEGLEASNPQISMGQAMQAKLDNLAN
                     KWSEASHEK"
     gene            6749..7408
                     /locus_tag="VN496_09700"
     CDS             6749..7408
                     /locus_tag="VN496_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF17267.1"
                     /translation="MKNNRISAFALMALIALACFRVPAGAEDWTPLSRAKALEQTRST
                     QLDKRRIAYGRLAEVGTMEDIPVLLTALWDEQALIRGIAEQSVWGIWMRTDDPVVDPL
                     FQTGILLIAEDKADTAIEKLNQVIALKPDFAEAWNRRGNAYAALGDEERALADYEHTI
                     ALNPYQFGTMESCAEIWLMRSNYRKAAEYFRRALDLNPNLADAAEALHELEAKLENDR
                     I"
     gene            complement(7591..8640)
                     /gene="ltnD"
                     /locus_tag="VN496_09705"
     CDS             complement(7591..8640)
                     /gene="ltnD"
                     /locus_tag="VN496_09705"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HXF17268.1"
                     /translation="MRHTSTTSDFSSWVGESGRLTAGRYARKESPRVQKQSNARQDRS
                     TQMATTKKQIAGVIGLGSMGLGVARTLLKKGFEVHAYDVRPEVSKAFEQEGGVAAKNP
                     AAVGSNAEVIIVLVVNADQTNEVLFGKDGAASTLTSGSVVIASSTVAPEFATDLGKRL
                     DAKGLLLIDAPVSGGAARAASGELSIMASGSKAAFRKADAVLKAIAVKVYELGEDAGT
                     GSKVKMINQLLAGVHIAASAEAMALAIRVGADPKVVYEVISNSAGSSWMFQNRVPHIL
                     DGDYTPRSAVNIFVKDLGIVLDSARKMTFPLPLTAAAHQLFLGAAGAGLGYEDDSAVV
                     KMYQAMSGIELPKKP"
     gene            8604..9671
                     /locus_tag="VN496_09710"
     CDS             8604..9671
                     /locus_tag="VN496_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF17269.1"
                     /translation="MKKNPKWLMYAACAMLLGSCHGNPHYSPVKSHHTEDGFRNNYPV
                     PERGSFWKWQWDRWTKGVPDDPKEGYAFASMKPDVAFLASNRSEPTLTWIGHASFLVQ
                     AGGVNMLTDPIFSERASPVSFAGPKRHVQAMLDMRSLPHVDAVVISHNHYDHLDLPTV
                     KQLNIQPGGAPRFFVPLGLKQWFGSNGIENVTELDWWDSAEFMGLKLTLAPVQHWSSR
                     TPWDRNKTLWGSWVIEQAHMRLFFGGDFGYSPDLAAIGQHFGNVDLAMLPIGSYEPRW
                     FMSAMHVNPEEAVRAQQDLHARYAVGMHWGTFRLTDEVLDEPPRKLADAMAQAGVAPE
                     RFFVMRHGEMRKLDFLTPGGQ"
     gene            9668..10756
                     /gene="queG"
                     /locus_tag="VN496_09715"
     CDS             9668..10756
                     /gene="queG"
                     /locus_tag="VN496_09715"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXF17270.1"
                     /translation="MKASQNSASPALPGAAQLVSKIRKWAAELGFQQVAVADCDLSEA
                     EPRLQQWLARGWNGDMEYLSAHGTRRTRPAELVPGTVRVLSLRMNYLPADAADSGEVL
                     ADGSRAYVARYALGRDYHKVLRGRLQHLCDRIAAVTPFQYRVFTDSAPVMEVELASKA
                     GLGWRGKHTLVLNREAGSWFFLGEIYTDLPLPVDAPQPEHCGTCSRCIDVCPTRAIVG
                     PYQLDARRCISYLTIEHKGDIPEELRPLMGNRIYGCDDCQLVCPWNRYAQKAQAPDFA
                     VRNRLDAAGLVELFSWDEQQFERRMEGSAIRRIGHERWLRNLAVGLGNAPTSSAVIAA
                     LEARRDHPSVMVRRHVEWALQRHGEPRG"
     gene            10874..12253
                     /locus_tag="VN496_09720"
     CDS             10874..12253
                     /locus_tag="VN496_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXF17271.1"
                     /translation="MLIVGFGSVGQGSLPLILRHIDIEPEQITIITANAFGREIAGTL
                     GVKFIVEPIRSDTYVAQYDKYLGEGDFLVNASYDVNSLDTIEYCQQRDILYIDACIEP
                     WPGGHNNTDVPAALRTNYAYRDAALQMRNKYPKGPACVLMHGANPGLVSHLTKQALLN
                     VAKDMGIRKAEPKSRKQWAELAQRLDIKVIHIAERDSQLSARRKARDEFVNTWSSEAF
                     AGESLQPAELGWGTHEKNWPADGRTFNFGCGAAIYLERTGASTRVRTWTPLEGPMLGF
                     LISHGEATSIPDFYTVEKKGKAVYRPTCHYSYHPCDDAVLSMHEYIGNNYKFPPRYRL
                     LRDEISEGMDELGVLLMGHAKGAYWYGSQLTIEEARQLCPYNNATSLQVNIGYVSAVI
                     WAIKHPEAGIVEPDELPYKAMLEICRPYLGRVVGVYTDWTPLEERGVLYPEDVDSSDP
                     WQFKNFRVV"
     gene            12331..12981
                     /locus_tag="VN496_09725"
     CDS             12331..12981
                     /locus_tag="VN496_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXF17272.1"
                     /translation="MDLESSRTKLDRYPARGKHDFATVAAILDEGFFCHVGFVQNGQP
                     FVIPTGYGRKEGVLYIHGSAASRMLTEAEKGIALCVTVTLVDGLVLARAAFHSSVNYR
                     SVVILGQAEPVPAHEKLLALKTISDHIVPGRWESQRPVKQQELDQTRVLKLEIAEASA
                     KIRTGPPIDDEEDYALPIWAGTLDFSAPAGTPNPDKRLQPGVATPAHVLRYRRVKI"
     gene            complement(13037..13750)
                     /locus_tag="VN496_09730"
     CDS             complement(13037..13750)
                     /locus_tag="VN496_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HXF17273.1"
                     /translation="MKAPEVRALVFDVFGTVVDWRGSLIREGKALAAQKQLDVDWAAF
                     ADAWRGGYRPAMDKVRKGTLPWMNIDRLHRLILDDLLPQFGIHGLTEDEKEQLNRAWH
                     RLRPWPDSVRGLKRLKKKFVISTLSNGNVSLLTNMAKNAGLPWDCVLSAELFHHYKPD
                     PESYLGAAAMLGFRPNEVMLVAAHKDDLRAAKGCGLHTAFVRRPKEAGPNVKPDLTSD
                     PGFDYNSNDFLDLADQLRA"
     gene            complement(13835..>14370)
                     /locus_tag="VN496_09735"
     CDS             complement(13835..>14370)
                     /locus_tag="VN496_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXF17274.1"
                     /translation="GAHARVEKLNLAVNYGYIQATFQSQLTLNSPSNSSANAAGDIQV
                     SPGDKIPGIPEHSVKLRADYAFTEKFSLGGNIVYFSSQYARGDENNQDVNGKVPGYAL
                     VNLDAHYQYNKRLSFFGRVDNLFDKDYETLGVLGDNFFNGPGRTFDATNVTNDQFRSA
                     GAPRAAWVGVRYAFEQK"
BASE COUNT         2928 a         4139 c         4343 g         2960 t
ORIGIN      
        1 tccagcgtca cgacccggac attcgctaat tcggaaacca gtgccttggc gagcgttacc
       61 ggcgtcatca agtcgcgttt gcccaggatc atcagcaccg ggcatctcag acttttggcg
      121 gctgccagtc cggctgaata gctgttgcag gcggtgaagt cggcatgcag cacgcccggt
      181 gcggaacgct ccagcaaccg catgcctgaa cccatcatcc acatgccggg cgtgcggttt
      241 ccgcccattt gcgcggctgc actatgcgaa taaacgttga tcatgtcgaa ggctgcgtgg
      301 tcgttggctt tcgagccgtt cagcagcggc gcggacaccg gcatgggaac cccgcaacca
      361 atcaatgcaa tccgcgatat ccggctggga tagcgcgaag cgcattccag cgcaatcaat
      421 gctcccatgc tgtggccaac cagcgcggca cgcgtcgcac cggcttcatc gagcaaccgg
      481 gctatccaga ctgccgcatc ctcgatagcg gtaaaggcgg gcgcgtgcga tttgccatgg
      541 ccgggaagat cgatcgccag ggcattccag ccgtggtagg caaaaaagcg gctttgcagc
      601 gaccatacgc tgtggtcatg cgccgcaccg tggataaaag caaccgtcgg cttgctggcg
      661 tcgaacgcgc ggttcgccgt ataggcgtat gtcttcctgt tgtcgaccgt caggtacatg
      721 gcaatgtcgt ggtggtgcag cagcgggccg caaaggatat acactgatgc caaccgcgcg
      781 caaccggaac agcatcatca agacgcgcgg gcgcaaacgg atgcgtcgtc gtgtggcgcc
      841 tgtcaatctg ccaagggagg aaagaatcat gtttcagaaa cgcagtgcaa tgatagtgtc
      901 gggaatggtg ctggcgttcg cggtcggcat tgcggtcgga cagaatgcag caccgacggc
      961 aaacaaaggc gtcaacgtgg gcgcgcccac gttcctcgac ctcacggacg agatcgacag
     1021 cgttgcgggc aggcaattgc gcatgcgggt ggtgtccatt gatccgggcg gcgtggtggc
     1081 gctgcacagt cataacggac gcccggccgt cgcttatgcc ctgcagggca ccttggttga
     1141 acacctcgag aacggcgatg tgcgcgagca tccgcaaggc gaatcctgga ccgaggacaa
     1201 gaacactacg cattgggcgg aaaacaagag caaccagccg gtaaaggtca tcgccgtcga
     1261 cgtgttcaaa ccctgagccg ctgtccggag ccggttccga agtcaaacaa ggatcgccat
     1321 ggagcatgcg gctgtggtcg cttgttccat ggcgagggtg gcttgtaccg gagcgactat
     1381 tttttggcgg gatctttcag cgagcatttc cagccggtca ctgtcgaatt ctttggaatc
     1441 gcttgcggaa gattcgccat gatggatggc aacgaatcgc caaaactcct cttgcattct
     1501 tcgagagtct tgatattcgg attgaacacc acgtagcggt ctatatccgc atcgttcttg
     1561 agcgagatct cgacatacac gcccggcgcc tgttcctgct tgcatcctgc caagcctaac
     1621 gcggatacca tagcgaaacc aattgcggcg ttcttcattg gaatgctgct ttccgcatca
     1681 agacattttg cggttcagga tttttgcgag gcgcgcaacg cccggctcaa atcctcgatc
     1741 aggtcttcct gattctcgag cccgatcgac aagcgcacgg tgccctcggt gatgccagac
     1801 gccagaagag cggcgttatc catgcggtag tgcgtcgtgg tggcggggtg gatcaccagc
     1861 gacttggcat cgcccacatt ggccagatgt gaaaagatct tgagtgcttc gatgaatcgc
     1921 cgtcctgcag cccggccgcc tttgagactg aacgtaaaca cggcgccaca tccgcgcggc
     1981 aaaatggctt ttgccagctc atggtccgga tgcgatgcga gttccggata ggaaaccgat
     2041 tcgacggcgt cgtgcccgga caggaactgc acgatggctc gcgtgtttgc agtgtgtttt
     2101 tccatgcgca ggcccagcgt ctccatgccc tggagaatct ggaaggcgtt catcggactc
     2161 atcgctgcgc cgaaatcgcg aatgccttca cgccgcgcgc gcaacagaaa ggctgcggtg
     2221 gtggattcct cgctgaagac catgccatgg aaccccgcat aaggttcggt caaagtcgga
     2281 aatttccccg acttctccca gtcgaacttg ccgctgtcca caatgagtcc gccgatcgct
     2341 acgccgtgac cacccaggaa tttggtggcc gaatggaaga caaggtccgc acctagatca
     2401 aacgggcgca gcagataagg tgtcgtaaag gtcccatcca ccagcaatgg cagaccagct
     2461 tcgtgcgcta tttcggcaac ctgcgcaatg tcgagcacat cgagcccggg gttgccgagg
     2521 gtttcgccga acacaagccg ggtatcggga cgaatggcct tgcgaaaagc atccatgtcg
     2581 cgcgggttga cgaacgtggt ttcgatgccg aagcgcggta gcgtgtactg cagaagattg
     2641 tgcgacccgc cgtagagcga acgcgaagcg acgatgtgag agccagcgcc cataagcgta
     2701 gcgatcgaca gatgcagggc ggcctgtcca cttgccgtgg cgatggcgcc gacgccgcct
     2761 tccagagcgg aaatgcgttc ttccagcacg gcgttggtcg gattcgaaat gcgtgtatac
     2821 acgtgacccg cgcgctccat gttgaatagc gaggctgcgt ggtccgtatc cttgaagacg
     2881 tacgaagtcg tgaagtacac cggctgcgcg cgtgcgccga actgcggatc gggctgctgc
     2941 ccggcatgca ggctcagagt atcgaactga taggttttcg gtcctggcat cggatggctg
     3001 atgacggcga tgaatcagta aaaggccccg cgtgcggtga ccggccagac gcattccacc
     3061 ttgtctgcac gcaggcccac gatccagtca taaaggttta cggtcgggtc gcaatggccg
     3121 gggaccaggc gcagtttgtc gccgacggtg acgtgagtgc cttgggcaag cttcaacacc
     3181 ccatgctcgt cagatgcctt tagaaattct acgcccggca gatccgcgac aagcggcatg
     3241 cctgagtcca ccgacagcgc cttgagccct gcatccacga ctgcgcgctc ggggctcgca
     3301 tggctcatca ccgtcgccca gacgtaaaga ctctgctcaa aggcgtgcgt cggcttgcca
     3361 ttttcgccga gattacggtt gtaatcggca tccatgaaga tgtaggagcc cggctgaatt
     3421 tcgttgtaca cgccgcttgc cgcctccagc agatacgtgc cggtgccggc accggtgatg
     3481 atttccggct tgagtccgtt tgcctgcagc gcttcaacgg taatgcgcac atattggacg
     3541 gcctgcgcaa tggcggcctt gcgctctgcc ggcgtgcgcg catgttgtgc ggcgccctga
     3601 taagcatgca gcccggcaaa gcgcagttgc ctgctggccg caatgcgctg cgcgagatcg
     3661 acgcatgcgg gtcccggttc aacgccgcag cgattgcctc cgacattgac ttccaccagc
     3721 acgtcgattt tcgctccggc cgcgcgcgcg gcggcgtcca tgtcgacgac gttctgttcg
     3781 ttgtcgacgc agacactgat ccgcgcacgc cgggccagcg ccgcgaggcg cgcgagcttc
     3841 ggcgcgccga ccacttcgtt gctgacgagc acgttcatta cgccgccttc caccatggcc
     3901 tcggcttccg atatcttttg gcagcacacg ccgatcgccc cgcgcgccat ctgtcgtagc
     3961 gcgatttcgg cacatttatg tgacttggca tgggggcgca cgcggatgcg caagcctgcc
     4021 aacgacttgt ccaggcggtc caaattacgc tcgaaggcgt cgagatccag cagcagcgct
     4081 ggggtatcca cttcggctag gctcatgccc gggcgggccg gaggagcagg acgcaggttc
     4141 attcggtggt cttgtttcgg gcttatattc gcgttcgcga tggaaacggc gatgctggcc
     4201 tagcgtcccg tttcacagtc ggtgcgcaag acgttgcctg cgctctctac gctccagttg
     4261 ttgccgtaag agccaaacac caggtccacg acgacagtca ggtttttctt ttccaccgca
     4321 tcgttgtagc tgcgcttttc cagtgccgca agggcctggt cccgcgactg gccttccttg
     4381 cgccatctca tcgcttccga ggcgaagctg ccgatgtcgg cgcaggtcat ggtaccgagc
     4441 gggtagggcg ccgccatggc tgcagtggaa agcattatca gcaagccaga cgcgattcgt
     4501 ttcgacatag ggaaatcctg cggaaagtga agttggcgga gtagaatttg tccgcggacg
     4561 gcttgcgcat tttaagcgtt cggacagggg aatgccatgt cactggaaca gtcggaaaaa
     4621 gccgtcgcaa cgcgttgcct gcttgccgcg ctgacctttg tcgttatctg cgccggcgtg
     4681 gatgccgcgc agccaggcaa caacgagacg cgcaaacagg cgctcgcgca attgcaggac
     4741 agtgacgcgg ccggacggct ggctgggtgc acggtgctgg ctgaaatcgg cagacccgaa
     4801 gatctgccgt tattacaggc tcgtctgagc gatgaagact accggattcg cgattctgcg
     4861 gaagcggcaa tatgggctat ctggagccgg tcgggcgatg cggccgtaga tgagctattc
     4921 agagagggcg tggaacaaat gcgcagcgca aacctcaaag cggcagcgga gaccttcagc
     4981 cgaatcattt cgatgcggcc tcaattcacc gaggcctgga acaagcgcgc caccgtttat
     5041 tttctgctgg gccaggacga tttgtcgctg aaggattgcg atgaggtggt gaaacgcaat
     5101 ccccagcact ttggcgtgct ggcgggttac gggcaaatct atctgcgcaa gggcgacctg
     5161 ccgcgtgcgc aagaatattt tgagcgcgca ctggcagtca atcccaatat ggggggcgtg
     5221 caagcctccc tggacgccat tcgcaagatc ctgatcaaac gcggcgaacg ctatatctga
     5281 gataacgcca cgcggtgtgg cggccctact cgcgcggccg gtatgtggag gttcggaaat
     5341 cacaatggca tcatggaagt gaacgacgca acgcaaccaa tgcctgacag cacagcgcgc
     5401 gccgtgctga gcacattgta cggcgggctt aacttgggtt tatttcgcca tcccggcgcg
     5461 gcgaggccct gcgtcacctc gactcagttc atcgtcatcg cggtcgcgtc cctcgcagta
     5521 agcggcgtct gctcttttgt cctgtccggg cccggcgcac tgttcaaccc gcaagcactc
     5581 gcgtcgcagc tgctgtgggt accgctggcg ttgctggccg gccatctggt gggtcggcta
     5641 ctcggagatg aacgctattt gctgcaggtc gcgacaacgg ttggcacgat cggtatcgcg
     5701 ctgtcggtcg catctggagc tgtctggttc gcagtggacc gcggctggat cagggttccg
     5761 gcagtgctcg gcgtgagcgg gatttatgat ttgttattcg cctggtgggc gatggcgatc
     5821 gttgttgctg taagacgcat gacgttctcg tcgtcccacc gcacactatg gccggtgttt
     5881 atcgtcacgc tgatggtagt tgtgcccgtg tatctggtaa cgcctgagcc gttatgggaa
     5941 gcgccgggtg aagacacgcc accggaaacc ggatcgcgcc cgtttggtga acgtgcgctt
     6001 tacgcgcaaa ctgaacttct gaggaaggcc gagcagcgac tgaaaccaga acgtgccggc
     6061 gtggaagatc tgtatttcgt tggcttcgca cctgacgctt cgcaggatgt gttcatgaag
     6121 gaaaccctcg ccatcggcag gctgatggac gcgcggttcg gggtggaagg gcgcagtatc
     6181 aagctcatca gccatcccgg cgtggtcgag acgttcccga ttgccacgct caccagtctg
     6241 cgtcatgccc ttcacgccat cggcagccgc atcaatccgg aagaggacgt ggtattgctg
     6301 catgtcactt cccacggctc ggaaaatcac gaattgagcg ttgctttctt tccgctcgaa
     6361 ctgcgggcga ttgccccgga cgatgtgcgc tctgcgctgg acgacgcagg catcaaatgg
     6421 cgcattgtgg tgatttccgc atgttactcg ggcggattca tcgaggcgct gcaggatgcg
     6481 catacgcttg ttatcaccgc ttcagacgcg acccatacct cattcggttg cggcaacgcg
     6541 ttcgatttca cttatttttc caaggcctat tttgacgagg cgttgcgcca caattattcg
     6601 ttcgagaagg cattcttcat agcccaaaaa gcgatcgcgg cgcgcgaaaa ggcagagggg
     6661 ctggaagctt ctaatccgca gatctccatg gggcaagcca tgcaggccaa gctcgacaac
     6721 ctggcgaaca aatggtccga ggccagccat gaaaaataat cgaatttcgg cgtttgcgct
     6781 tatggcgctg attgcgctgg cgtgctttcg tgttccggcc ggcgcggaag actggacgcc
     6841 gctgagccgg gcaaaggcgc tcgagcagac gcgcagcact cagctcgaca aacgccgcat
     6901 cgcgtatggc cgcctggcgg aagtcggcac gatggaagat attccggtgc tgctgaccgc
     6961 gctgtgggac gagcaggcgt tgatccgcgg catcgcggaa caatcggtat ggggcatctg
     7021 gatgcgcact gacgatccgg tggtcgatcc gttgttccag accggcatac tgctgattgc
     7081 cgaggacaag gcggacacgg cgattgaaaa gctaaatcag gtcattgcac tgaagccaga
     7141 ctttgcggag gcctggaacc gccgtgggaa tgcttacgcc gcgctcgggg acgaagaacg
     7201 tgcgcttgct gattacgagc acaccatcgc gttgaatccg tatcaattcg gcacaatgga
     7261 aagctgcgcg gagatctggc tgatgcgcag caactatcgc aaggcagccg agtatttccg
     7321 ccgcgcactg gacctgaacc cgaacctggc cgatgcagcc gaggcgctgc acgagctgga
     7381 agcgaaactc gaaaacgatc ggatctgaag tgccgtgaat atcgtgaatg cgtgaatgtc
     7441 gtgaatacgt gaataccgcg aatccgtaaa tgccgagtct cctgaacgat cacggatggt
     7501 gtaccggctt cgccgtaacc gtgctctgcc cattaatgcc ctaagtgcca aagcttaatc
     7561 gatattcacg tattcacgga ttcacggcgt ttacggcttc ttcggcagtt ctatccccga
     7621 catcgcctga tacattttca cgaccgcgga gtcatcttcg tatcccaggc cggcacccgc
     7681 cgcaccaagg aacaactggt gcgcggccgc agtcagaggc agggggaaag tcatctttct
     7741 ggcggagtcg agtacgatgc caagatcctt gacaaaaata ttgacggcgg aacggggcgt
     7801 gtaatcaccg tcaaggatat ggggcacgcg gttctggaac atccaggatg aaccggcgct
     7861 gttggagatg acttcgtaaa cgacctttgg atcggctcct acccggatgg cgagcgccat
     7921 ggcttcggct gatgcggcaa tgtgcacacc cgcgagcagt tgattgatca tcttgacctt
     7981 ggagccggtg ccggcgtctt caccgagttc gtaaaccttg actgcaatag ccttgagaac
     8041 cgcatcggcc ttcctgaatg ccgctttcga accggaggcc atgattgata attcccccga
     8101 agcggcacga gccgcgcctc ccgaaaccgg tgcatcaatc aatagcaggc ctttcgcgtc
     8161 caggcgctta cccaggtcag tcgcaaactc cggcgcaacc gtgctgctcg caatcacgac
     8221 agagccgctg gtcagtgtag atgcggcgcc gtccttcccg aacaaaactt cattggtctg
     8281 gtcggcattg accaccagca cgataatgac ttcggcattg ctgccgacgg cggcaggatt
     8341 ctttgcggca acaccgcctt cctgctcgaa tgctttcgag acttctggtc gtacgtcgta
     8401 ggcatgtact tcgaatcctt tcttcaacaa ggtccgcgcg acaccgagac ccatcgatcc
     8461 cagtccaatg acgcctgcga tttgtttttt tgttgtggcc atttgcgtgc tcctgtcttg
     8521 tcgagcgttc gattgttttt gcacccgcgg cgattctttg cgagcatagc ggcccgccgt
     8581 aaggcggccc gactcgccca cccatgaaga aaaatccgaa gtggttgatg tatgccgcat
     8641 gcgccatgct gcttggatct tgccacggca atccgcatta tagtccggtg aaatcgcatc
     8701 acaccgaaga cgggtttcgc aataactatc ctgtgccgga gcgcgggagt ttctggaagt
     8761 ggcagtggga ccgctggact aagggcgtgc ccgacgatcc gaaggaaggg tacgcctttg
     8821 cctcaatgaa gcctgacgtc gcttttctcg ctagtaatcg cagcgagccg acactgacgt
     8881 ggatcgggca cgccagcttt ctggtgcagg cgggaggcgt gaacatgttg acagatccga
     8941 tattcagcga gcgcgcgtcg cccgtttcat ttgccggccc caagcgtcat gtacaagcta
     9001 tgctggatat gcgctcgctt ccgcacgtcg atgcggtggt gatctcccat aatcactatg
     9061 atcatcttga tctgccgacg gtcaagcaac tcaacataca gccgggaggc gcgccgcgtt
     9121 tttttgtgcc actgggcttg aagcaatggt ttggatcgaa cggcattgag aacgttaccg
     9181 aactcgactg gtgggatagt gccgaattca tgggactgaa actgactctt gcgcccgtac
     9241 aacattggag ttcgcgcacg ccatgggacc gtaacaagac gctgtgggga tcgtgggtca
     9301 tcgagcaggc gcatatgcgg ctgttctttg gcggcgattt tggatattcg ccggacctgg
     9361 ccgcgatcgg ccaacacttt ggcaacgttg atttggcgat gctgcccatt ggcagttacg
     9421 agccgcgctg gttcatgagc gcgatgcacg tcaatcccga agaagccgtg cgtgcgcagc
     9481 aggacttgca tgcgcgttat gcggttggaa tgcattgggg caccttcagg ctgaccgacg
     9541 aagttctgga cgagccgccg cgcaagcttg ccgacgccat ggcgcaagct ggtgttgcac
     9601 cggaacgctt cttcgtcatg cggcatggcg aaatgagaaa actggatttt ctgacacccg
     9661 gcgggcaatg aaggcctctc aaaacagtgc atcgcccgcc ttgccgggcg ccgcccaact
     9721 ggtttcgaag atcaggaaat gggccgccga gcttggtttt cagcaagtgg cggtcgctga
     9781 ctgcgatctt tcagaagcag agccccgcct gcagcaatgg cttgcgcgcg gctggaacgg
     9841 cgacatggaa tacctgtccg cacacggcac gcggcgcacg cgcccggcag aactggtgcc
     9901 gggtaccgtc cgcgtcctga gcttgcgcat gaattatctc cctgcggacg ctgcagacag
     9961 cggggaggtc ctggcagatg gcagccgggc ttatgtcgca cgttatgcac tcggacgcga
    10021 ttaccataag gtgctacgcg gccggctgca acacctgtgc gatcgcatcg cggcggttac
    10081 gccgtttcag taccgcgtat tcaccgacag tgccccggtc atggaagtgg aactggcaag
    10141 caaggcaggt ttgggctggc gtggcaagca cacgctggtg ctgaatcggg aagccgggtc
    10201 atggtttttc ctgggcgaaa tctacaccga tctgccatta ccggtggacg cgccgcagcc
    10261 ggaacattgc ggcacctgca gccgatgcat cgatgtttgt cccactcgtg cgattgtcgg
    10321 tccctatcaa ctggacgcgc ggcggtgcat ttcctatctg accatcgaac acaagggcga
    10381 tatcccagag gaattgcggc cgctgatggg caatcggatc tacggctgcg atgactgcca
    10441 gctggtctgc ccctggaacc gctacgcgca aaaggcgcaa gcgccggatt tcgccgtgcg
    10501 taaccggctc gacgcggctg ggctggtgga gctgttctcc tgggacgagc aacaattcga
    10561 gcggcgcatg gaaggcagcg caatccggcg cattggacat gagcgctggc tgcgcaatct
    10621 ggccgtcggg cttggcaacg cacctacttc atctgccgtc attgcggccc ttgaagcgcg
    10681 gcgcgaccat ccttctgtca tggtccggcg gcacgtcgaa tgggcgttgc agaggcatgg
    10741 tgagccccgg ggttgagatt gccgccgact tcgtctataa cgtgcccctc gcgcggcgaa
    10801 cgtccgcgcc atctgaccag gagcgcaaga caaattggcc ggccataaga aatatgcgtc
    10861 cttcaaagga agaatgctca tcgtgggttt cggatctgtg ggccagggat cgctgccgtt
    10921 gattctcagg cacatcgaca tcgagcccga acagatcacc atcatcaccg caaacgcttt
    10981 cggtcgtgaa atcgcgggaa cgctcggcgt gaaattcatc gtcgagccga tcagaagcga
    11041 tacttacgtt gcgcagtatg acaaatatct tggcgaaggc gacttcctgg tcaacgcgtc
    11101 gtatgacgtg aacagcctgg ataccatcga gtattgccag cagcgcgaca ttctttacat
    11161 cgatgcctgc atcgagccct ggcccggcgg acacaacaat accgatgtgc cagcggcgct
    11221 gcgcaccaat tatgcgtatc gggacgccgc cctgcagatg cgcaacaaat atccgaaagg
    11281 gcctgcctgc gtcttgatgc acggggcgaa tcccgggctg gtctcgcacc tgaccaaaca
    11341 ggctttgctc aatgtggcga aggacatggg gatcagaaaa gccgagccga aaagccgcaa
    11401 gcaatgggct gagctggcgc agcggctcga tatcaaagtg atccacattg ccgagcggga
    11461 tagccaattg agtgcacggc gcaaagcgcg tgacgagttc gtcaatacgt ggtcgagcga
    11521 agcctttgcc ggcgagtcgc tgcagccggc cgaacttggg tggggcacgc acgaaaagaa
    11581 ctggccggca gacggacgca cgtttaactt cggctgcggc gcggcgatct atctggagcg
    11641 cacgggcgca tccacccggg tgcgcacctg gacgccgctc gagggtccga tgctgggctt
    11701 cctgatctca catggcgagg cgacatccat cccggacttc tacaccgtgg agaaaaaagg
    11761 caaggcggtc tatcgcccaa cctgccacta ttcctaccat ccgtgtgacg acgcggtgtt
    11821 gtccatgcat gaatatatcg gcaacaacta caagtttccg ccgcgctacc ggttgctgcg
    11881 cgatgagatt tccgaaggca tggatgagtt gggcgtgctg ctgatgggac acgcgaaggg
    11941 cgcttactgg tacggctctc agctcaccat cgaggaagcg cgccagcttt gtccctacaa
    12001 caacgcgacc agcctgcaag tcaacatcgg atacgtgtcc gctgtgatct gggcgatcaa
    12061 gcatcccgaa gcgggcatcg tggaaccgga tgaattgccg tataaggcga tgctggagat
    12121 ctgcaggcct tatctgggca gggtcgtcgg cgtttacacg gactggacgc cgctcgaaga
    12181 acgcggagtg ctgtatccgg aagacgtcga tagcagcgac ccctggcagt tcaagaattt
    12241 tcgggtggtg tgaaaccggg gccacgggtt tcattcgcag actgctgttc aacacatcaa
    12301 gagttaattt gaacccacaa gagttttgcc atggacctcg aatcgagccg aaccaaactt
    12361 gaccgttatc ccgcgcgagg caagcacgac tttgcaacgg tagcagcgat tctggacgaa
    12421 ggctttttct gtcacgtcgg cttcgtgcaa aacggtcagc ccttcgttat tccaaccggc
    12481 tatggccgca aggaaggcgt gctctatatc catgggtcgg ccgcgagccg gatgctgact
    12541 gaagcagaga agggaatagc cttgtgcgtc accgttacct tggtcgacgg cctggtgctc
    12601 gcacgtgcgg cgtttcactc gtcggtgaat tatcgatccg tggtcattct cggccaggcc
    12661 gagccggttc cggcgcacga aaaattgctg gcgctaaaaa ccatctccga tcacatcgtg
    12721 ccaggtcgat gggagagcca gcgtccggtc aaacagcaag agctcgacca gaccagagtg
    12781 ctgaagctcg agatcgcaga agcctcggcg aagatccgga ccggaccgcc gatcgacgat
    12841 gaagaggatt acgcgctgcc gatctgggcg ggcacacttg atttttccgc tcctgcaggc
    12901 acgcccaatc cggataaacg gttgcagccc ggtgttgcta cgcccgcaca tgtgctgcgt
    12961 taccgtcgcg tcaaaatctg aagcgctcgc cggtcggata tttgtcgggc acgagggtca
    13021 cacaccgcgc ggctctttac gcgcggagtt gatccgccag atccaggaaa tcgttgctgt
    13081 tgtagtcgaa gcccgggtct gacgtgaggt ccggtttgac attcggcccc gcttctttcg
    13141 gacggcgtac gaacgcggtg tgcaacccgc agcctttggc tgcgcgcaga tcatccttgt
    13201 gcgcagcgac cagcatcact tcattcggcc tgaatcccag catggcggct gcacccagat
    13261 aactctccgg atccggcttg taatggtgaa acagctccgc cgatagcacg cagtcccatg
    13321 gcaagccggc attcttggcc atgttggtca gcaatgaaac attgccattc gatagcgtgg
    13381 atatgacaaa ttttttcttc aggcgcttga gcccgcgtac tgaatccggc cacggcctca
    13441 gtcggtgcca cgcccgattg agctgctcct tttcgtcctc cgtgagtccg tggatgccaa
    13501 attgcggaag caaatcgtcc aggatgagtc tgtgcaaccg gtcgatattc atccagggca
    13561 gagtgccttt gcgcaccttg tccatggctg gacggtagcc gccgcgccag gcatcggcga
    13621 acgcagccca gtcgacgtcg agctgttttt gcgcagcgag ggctttgcct tcgcgaatga
    13681 ggctgcctcg ccaatcgacg actgttccga acacgtcgaa caccagtgcg cgtacttcgg
    13741 gagctttcat gctgtgcaat ggctcaatcg aggtaaatcg ttcggattat agacggctca
    13801 catggccagg ctgagcagaa tattcattgg tggatcattt ctgctcgaat gcatatcgca
    13861 cgccgaccca tgctgctcgc ggcgctcccg cggagcggaa ttggtcgttg gttacgttgg
    13921 tggcgtcgaa cgtgcgtccg gggccattga agaaattgtc acccaacacg ccaagcgttt
    13981 catagtcctt gtcgaacaga ttgtcgacgc gcccaaaaaa ggacaaccgc ttgttgtact
    14041 gatagtgcgc atcaagattc accagcgcat agccgggcac tttgccgttg acgtcctggt
    14101 tgttctcgtc gccacgggcg tactgcgagg agaaatacac gatgttgccg cccaaagaga
    14161 atttctcggt aaaggcgtaa tcggcccgca gcttcaccga gtgctccgga atgccaggta
    14221 tcttgtctcc aggagaaacc tggatatcgc ccgctgcatt tgccgacgag ttgcttggac
    14281 tgttcagcgt aagctgagac tgaaatgtag cctgaatata gccatagttc acggcaaggt
    14341 tgagtttctc cacgcgtgca tgtgcgccca
//
LOCUS       DATMZQ010000298         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1279006, whole genome shotgun sequence.
ACCESSION   DATMZQ010000298 DATMZQ010000000
VERSION     DATMZQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279006"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VN496_09740"
     CDS             complement(<1..459)
                     /locus_tag="VN496_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXF17275.1"
                     /translation="MTDDLKAKLNTARRRIAALIVVVFAIIWFCNLGYRSLVRPDEGR
                     YAEIAREMAITGDWVTPRLNGIKYFEKPPLQYWITAAAYKAFGESEWTARLWTALAGF
                     LGVFAVAFAGRRLFGRAAGFCAALVLSSSLLYAGIGHVNTLDMGVTFCLTL"
     gene            complement(558..1199)
                     /locus_tag="VN496_09745"
     CDS             complement(558..1199)
                     /locus_tag="VN496_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXF17276.1"
                     /translation="MNLSAMRSLLFFLMLVIVPVSVGATQTILVLGDSISAGFGLAQG
                     EGWVALLEQRIKRDKLDYQVVNASISGETTLGGRNRIANALEEYRPAVVVVALGGNDG
                     LRGNPLEETRRNLIAIVEACRKKGAKVVIAGMRIPPNYGPVYTRRFESLFTEVARQQN
                     VSLVPFMLQGFADDRNMFQADGIHPAAAAQPRILNNVYHRLRPLLLPRPRRQT"
     gene            1183..1878
                     /locus_tag="VN496_09750"
     CDS             1183..1878
                     /locus_tag="VN496_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF17277.1"
                     /translation="MADKFMPPIVRAAGLTKQVFTGTSALTILTDVSFEIERGEAVAI
                     LGASGSGKTTLLALLAGLDVPTSGTVDIDGQDLFALDEDGRARLRRKRIGFVFQSFQL
                     LPALTALENVMLPLELAANPDAKNRASDWLARVGLGQRITHYPRQLSGGEQQRVAVAR
                     AFAADPQLLLADEPTGNLDSATGEEIISLMFELNRVAHTTLLLVTHDELLARRCPRRI
                     GLVAGRLSSDERR"
     gene            1865..>2363
                     /locus_tag="VN496_09755"
     CDS             1865..>2363
                     /locus_tag="VN496_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF17278.1"
                     /translation="MSGADLRPFAVLMLAARMLMRDWRAGEQRLLALALVLAVASVTT
                     VAFFVDRVDRSLRTESNQLLGADLVVVSDHPIEAFLVQNAERLGLASTTAVRFPSMTQ
                     FGGRTLLTEVKAVTAGYPLRGKLRIRAAETAADVEPRRIPSTGEVWVDDRLLRRLAIK
                     IGERIV"
BASE COUNT          465 a          693 c          723 g          482 t
ORIGIN      
        1 cagcgtcaga cagaaggtga cgcccatatc cagggtattg acatgcccga tccccgcata
       61 tagcaggctg ctggacagga ccagtgccgc gcagaatcct gcggctctgc caaacaggcg
      121 cctgccggca aaagccacgg cgaatacgcc aaggaatccg gccagggcgg tccacagccg
      181 tgctgtccat tcgctttcgc cgaaagcctt ataggctgca gcggtgatcc agtattgcag
      241 cggtggcttt tcgaaatact tgatgccgtt cagccggggc gtcacccagt ccccggtgat
      301 cgccatttcg cgtgcgattt ccgcatagcg gccttcgtcc ggacgcacca ggctgcgata
      361 gccgaggttg cagaaccata tgatggcaaa gaccacaaca ataagtgcgg cgatgcgtcg
      421 cctggcggta ttcaactttg ctttgagatc gtctgtcatt gcctgcgctc tatgccaacg
      481 ctaaaaaaac agaggcaggc cgcgtgacgc ggtatgctca tgacccggtg ctgccttgtc
      541 gtgacgccgg tgaggcgcta tgtctgacgc cgtggcctgg gcagtagcag cgggcgcagg
      601 cgatgataaa cgttattcag gatccgtggt tgcgccgcgg cagcgggatg gatgccatcg
      661 gcttgaaaca tattgcggtc gtccgcgaag ccctggagca taaagggaac cagcgaaaca
      721 ttctgctgtc tggcgacttc ggtaaacagg ctttcgaaac gccgtgtgta caccggccca
      781 tagttggggg gaatacgcat tccggcgatc acgactttgg cgcctttctt gcggcaggcc
      841 tccacaatcg cgataagatt gcgccgggtt tcttcaaggg gattaccgcg caagccgtca
      901 ttgccgccaa gcgcaaccac tacgacagcg ggtcgatatt cctcaagagc gtttgcaatg
      961 cgattgcggc cgccgagcgt ggtttcaccg ctgatactgg catttaccac ctggtaatcg
     1021 agcttgtccc gcttgatgcg ctgctcgagt agcgcgaccc aaccttcgcc ttgcgccagg
     1081 ccgaaacctg cagagatgct atcaccgaga acgagtatgg tctgtgtggc tccaaccgac
     1141 accggaacga taaccaacat cagaaaaaaa agaagcgacc gcatggctga caaattcatg
     1201 ccgcctattg tgagggcggc cggtttgacg aagcaagtat ttaccggtac ctccgcgctg
     1261 accatcctca cggatgtttc cttcgagatc gaacgcggcg aggccgtcgc cattctcggc
     1321 gcttcgggct ccggcaagac cacgctgctg gcgctgctgg ccggattgga cgtgccaact
     1381 tcgggcaccg tggacatcga tggacaagat ctgtttgcgc tcgacgagga cggacgcgca
     1441 cgcctcaggc gaaaacgcat cggttttgtt ttccagtcat ttcagttgct gcccgccctt
     1501 acggcactgg aaaacgtcat gctgccgctg gaactggccg caaatccgga tgcaaagaat
     1561 cgggcctcgg actggcttgc gagagttgga ttgggccagc ggatcaccca ctatcccagg
     1621 caactctccg gcggagagca acagcgggtt gcagtggccc gtgcgtttgc cgctgatcca
     1681 caactgctgc tggctgatga gcccaccgga aacctcgatt ccgcgaccgg agaggaaatc
     1741 atttcgctca tgttcgaact caatcgcgtg gcgcacacca cgctgctgct cgtcacgcac
     1801 gacgagcttc tggcccgccg gtgtccgcgc cgcattggac ttgtggcggg acggctgagc
     1861 agcgatgagc ggcgctgatc tgcggccgtt tgccgtgctg atgctagccg cgcgcatgct
     1921 gatgcgggat tggcgcgccg gcgagcaacg gttgctcgcg cttgcgctgg tgctcgccgt
     1981 ggccagcgtg accaccgtgg ccttcttcgt cgatcgagtc gaccgttcgc tcaggactga
     2041 gtcgaaccag ttgctgggcg cggacctggt cgtcgtatcc gatcatccga ttgaagcgtt
     2101 tcttgttcag aacgccgaac gcctgggatt agcttcaacg actgcggtac gttttccaag
     2161 catgacgcag ttcggtggtc gcacgctgtt gactgaagtg aaggcagtta ctgcgggata
     2221 tccgctgcgc ggcaagctgc gtatccgggc cgctgaaaca gcggcggatg tggaacctcg
     2281 ccgcattccg tccacgggtg aggtatgggt ggacgatcgg ttgctgcgca ggctcgcgat
     2341 taaaatcggt gagcgcattg tgc
//
LOCUS       DATMZQ010000299         4467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2661245, whole genome shotgun sequence.
ACCESSION   DATMZQ010000299 DATMZQ010000000
VERSION     DATMZQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661245"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VN496_09760"
     CDS             <1..312
                     /locus_tag="VN496_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF17279.1"
                     /translation="DPKTSKGEGLRAPNGVVQDVRNYWASGKPYYDPAAIRVPVLLIK
                     AEWDQDTPAYMAQALFPLLKNAAAKRYVEIGEGTHTVIMEKNRLQLFHEVQLFLEEPV
                     K"
     gene            complement(376..1392)
                     /locus_tag="VN496_09765"
     CDS             complement(376..1392)
                     /locus_tag="VN496_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17280.1"
                     /translation="MKTLPDPRRYALSSTNPLVRLAQQSAASAELAAALHQALATGND
                     VVIEQALAEASSYAIFRVLSKALEHALKAPDDSVMHLRLFAIPILIVTGGRAPAVVPG
                     VVPDIEEVKTLFESHGTLGPIKTFGLSASLVSPAGLAALKFSLLYQRQRVSALESFPP
                     LDIPPQDLLIERSEEQAWLRFMTGVSLTSADAPGFTETAGNISAWGMAFTRTLADQLT
                     QPGLSLLPIPRLPASPRQALEEGTFALYELCLQLFLSGALRRFRARAGEPDAAVFACD
                     DASVRVRLSSPFDTSLSEQYVWPLQPGHDLALVGQAVFSLLDDCRVQNVQLSEAVEPV
                     SVSH"
     gene            1473..1823
                     /locus_tag="VN496_09770"
     CDS             1473..1823
                     /locus_tag="VN496_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF17281.1"
                     /translation="MKHINWDKIPAEKLNDKFVRKLAWDGKIMIGQTLVEKGYVVPLH
                     SHDNQQVTFVTSGVWRFKLENRTVEVGPNEMIFIPANVPHTAEALETLVAYDVFTPPR
                     EDWINGDDAYLRTR"
     gene            complement(1869..2345)
                     /locus_tag="VN496_09775"
     CDS             complement(1869..2345)
                     /locus_tag="VN496_09775"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF17282.1"
                     /translation="MKRFILLCALLLSFAARAGLEEGTEAYSTGDYAKALVEFRALAD
                     QGNSDGQYFLGFMYHNGFGVKRDQAEALKWFQKAAHQGDARAQYYVGIMYAAGQGVQK
                     DLQMADMWLTLSASNPKSSYRDSLYTKEEIGKIERKMTPEQIAQAKESVKNWKPQN"
     gene            complement(2705..3979)
                     /locus_tag="VN496_09780"
     CDS             complement(2705..3979)
                     /locus_tag="VN496_09780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXF17283.1"
                     /translation="MELLGGRGIVVERFPPFTAIGISLRNFAVPLVLWWLTILLAGHA
                     VFPDLLSGLQVHGPYIVFLTGIWLSLAFRQGRAFFVLLTLAVAYLAYVFFKEQATSQF
                     TARAVYAAVCVLIPINIAVYTLLRERGALNVAGLRRMSLPLLEIGAVAAIVIFDATDL
                     TAALYSPFYAHAALAGLHIPQLGIVCMIAAVIISVACAVARGSAVDAAFATAVALFAL
                     ACIDAPPAGAYGWFCSMAGAIVAIAVVQDSYRMAFYDELTGLAGRRALNERMQSLDGD
                     FTVAMIDIDNFKAFNDTWGHKVGDQVLKLVAARLQRVRGGGKAYRYGGEEFTIVFPGK
                     RAMSLKYPLEALRKEIETYQIRLRESGRRAGAPDVARPDPEDPKCISVTISIGVAERN
                     NRFEMPDRALKAADKALYRAKSEGRNRIVGPG"
BASE COUNT         1025 a         1285 c         1249 g          908 t
ORIGIN      
        1 gatcccaaaa caagcaaggg ggaaggactg cgcgcgccca atggggtcgt tcaggatgtt
       61 cgcaactact gggcgtccgg caaaccttac tatgatccgg cggcgattcg cgtacctgtt
      121 ctgctaatta aagcagaatg ggatcaggac acgcctgctt acatggcgca agcgttattc
      181 ccgctgctga agaatgcagc cgccaagcgc tacgtggaga taggagaagg aacgcacacc
      241 gtcatcatgg aaaaaaaccg cctgcaactg ttccatgagg tccagttgtt tctggaagaa
      301 ccggtcaaat agaattaccc gcggccttgc agaaggcagg caattacgta cccgttgcct
      361 cgcaaatcgg gtgcatcagt gcgaaactga aaccggctca accgcctcgg agagctgaac
      421 attttgcacc cggcagtcgt ccagcagact gaatacggct tggcctacca gcgccaggtc
      481 atgtccgggc tgcagcggcc agacatattg ctccgacaaa gaagtgtcga aaggcgaact
      541 caagcgaacc cggacgctgg catcgtcgca tgcgaacacg gcagcatcgg gttcaccagc
      601 acgcgcgcga aatctgcgca acgcaccgct cagaaaaagc tgcaaacata gttcgtagag
      661 cgcaaaggtg ccctcttcca gcgcctgccg cgggcttgcg ggtagtcgcg gtatgggcag
      721 cagcgacagg cccggctgtg tcaactggtc cgccagtgtc cgggtgaacg ccatgcccca
      781 ggcgctgata ttgccggcgg tttcagtaaa gccaggcgcg tcggccgaag tcagcgacac
      841 tccggtcatg aaccgcaacc acgcttgttc ttcgctgcgc tctatgagca ggtcctgtgg
      901 cggaatatcc agcggcggaa agctctccag ggcgcttaca cgctgccgct ggtagagcaa
      961 actaaatttt agcgctgcca gtcccgccgg cgacaccagg ctggcgctga ggccaaaagt
     1021 cttgatcggg ccgagtgtgc cgtgcgattc aaacagcgtc ttaacctcct cgatgtccgg
     1081 aaccacaccg ggcacaacgg ccggagcacg tccgcccgtc acgatcagaa tcggaatggc
     1141 aaacaggcgc aaatgcatta ccgaatcgtc aggcgcttta agcgcatgct ccagtgcctt
     1201 cgacaacact cggaatatcg cataggaact cgcttctgca agggcttgct cgatgacgac
     1261 atcgttccct gtagcaagcg cctgatgcag cgccgccgcc agctctgcgc tcgctgcaga
     1321 ctgctgagcc aggcgcacca ggggattggt cgaggacaat gcgtaacggc gcggatcagg
     1381 cagggttttc acgtgaatcg aggttatcgg gaggttcgca tcccggcaaa atgttatgct
     1441 cgaaataatc accgactaca gagaggctcg gcgtgaaaca tatcaattgg gacaaaatcc
     1501 cagctgaaaa attaaacgac aaatttgtcc gcaaactggc ctgggacggc aagatcatga
     1561 ttggccagac gctggttgaa aaaggctatg tcgtgccgct gcattcgcac gacaatcaac
     1621 aggtcacctt cgtgacgagt ggtgtctggc ggttcaagct ggaaaacagg acggttgagg
     1681 tcgggcccaa cgaaatgatc tttattccgg ccaatgtccc gcatacggcc gaggccttgg
     1741 aaacgctggt tgcctacgac gtgttcacgc caccgcgcga agactggatc aacggagacg
     1801 acgcctacct gcgcacaagg taaaccgctc acaagcgggc caataacgcg tcttctaccg
     1861 aagcgcggtc aattctgcgg cttccagttc ttgaccgatt ccttcgcctg cgcgatctgt
     1921 tcgggcgtca ttttcctctc gatcttgcca atttcctctt tggtatagag agaatcgcgg
     1981 tagctgcttt ttggattcga agccgaaagg gtcaaccaca tgtcggccat ctgcagatcc
     2041 ttttgcacac cctgcccggc tgcatacatt attccgacgt agtactgtgc gcgcgcatct
     2101 ccctggtgcg ccgccttctg gaaccatttc agcgcttcgg cctggtcgcg cttgacccca
     2161 aaaccattgt gatacatgaa cccgaggaaa tattgcccat ccgagttgcc ctggtccgca
     2221 agggcgcgaa actcgaccag tgccttggcg tagtcgccgg ttgagtaagc ttctgtccct
     2281 tcctcaaggc cggcacgggc ggcaaacgac aacagcaagg cgcacagcag gataaaacgc
     2341 ttcattgaat tccccgaaca attggattaa agacagattg tagcgcttca atggcgagct
     2401 gagccgcgaa gccttgacgg ggacggtgag gcgccggggc tgtcgtccaa tttgtggctg
     2461 cgattgcgtg aatcgcaact gagccctgag tgggctctgc aggtgggagg attgattgac
     2521 ggtgccccac ttgctgccaa tgggtccgta ctcacaaggc ggtgataatc gccttttatc
     2581 gccttcaccg tgagcgactg aaattcagcc ccggccgcca gcaaaaagtt tcgtcctgat
     2641 ggttccgtgt gtaaagagac aagttgtaag agtccatgac tacggcgtca ctttgccagc
     2701 ggctttagcc tggtccgacg atcctgttgc gaccttcgga ttttgcgcga tagagcgcct
     2761 tatctgccgc ttttagcgcc cggtccggca tctcgaaacg attgttgcgc tctgcgacac
     2821 cgatgctgat cgtgaccgat atgcacttgg ggtcttctgg gtcaggtctc gcgacatcgg
     2881 gcgcgccggc gcgccgcccg ctctcacgca acctgatctg gtaagtctca atttctttac
     2941 gcaacgcttc aagcgggtat ttcagactca ttgcgcgctt gcccggaaac acgatggtaa
     3001 attcttcgcc gccatagcga tatgccttgc cgccgccgcg cacgcgctgc agccgcgctg
     3061 cgaccagttt gagaacctgg tcacctactt tatggcccca ggtgtcgttg aaggctttga
     3121 agttgtcgat gtcaatcatc gccacggtga aatcgccatc cagcgattgc atgcgctcat
     3181 tcaacgcgcg gcgccctgcc agaccggtca actcatcgta gaacgccatg cgatatgaat
     3241 cctgcaccac cgcaatggcc acgattgcac ccgccatgga gcagaaccag ccgtaggcgc
     3301 cggccggtgg cgcatcgatg caagcgagcg cgaacaaagc aacggcggtc gcaaaggcgg
     3361 catcaaccgc cgatccacgc gccacggcac acgccacgct aatgatgacc gcggcaatca
     3421 tgcagacaat gcccaattgt ggaatatgca acccggccaa cgcggcatgc gcgtagaacg
     3481 ggctgtacag ggccgctgtt aaatccgtag cgtcgaagat cacgatagcg gcaactgctc
     3541 caatctccag tagcggcagc gacatcctgc gcagtcccgc gacgtttagt gcgccacgct
     3601 cgcgcaacag ggtgtaaaca gctatgttga tcggtatcag gacacagact gctgcatata
     3661 cggctcgtgc agtaaattgg cttgtcgcct gttctttaaa aaacacgtag gcgaggtagg
     3721 caaccgccag cgtcaataaa acgaaaaagg ccctgccttg acggaatgcg aggctgagcc
     3781 aaattccggt caggaatacg atatatgggc catggacctg caacccgctg agcaagtcgg
     3841 ggaaaactgc gtgtcccgcg agcaagatcg tcagccacca aagcacaagc ggcacagcga
     3901 aattgcgcaa cgaaatcccg atcgcggtga agggcggaaa ccgctccacc acaataccgc
     3961 gaccgcctag cagttccaca gcttgcgcac gtcacaacgt gtacgcagtt tcggatgcat
     4021 atgtaatatc cttctgctcg ccttgtgctg gctgctctat gcctgcgcgt tcggacatcg
     4081 tcggcgaaag agcaagcaac aagcaaaaga caatccagca gtgcatttgt tctgcgccag
     4141 gtatcaaatg gcatatttac tgatccagtt gttggtgtat ggcgacacaa ccgttaaatt
     4201 tttgcgtgaa ctcagctacc tcagacgacc gtgtgtcgtc tgtgagctac aggtcgaatg
     4261 gaataatcgc ggagatgtgg atcgcgtgta gctgctggag atcgcctcat gcaaacgtgc
     4321 acagcgacat ttgattgcgc ctgcagatca ggcgacgctt cattggcctg tgcgacgctt
     4381 tagtgatcca gtacttttgt tatggcgcag tggtgcgttc gacaaaaatt tgtgagcgct
     4441 cagatttgat cgcgccatgc ttggccg
//
LOCUS       DATMZQ010000300        10253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_248602, whole genome shotgun sequence.
ACCESSION   DATMZQ010000300 DATMZQ010000000
VERSION     DATMZQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248602"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VN496_09785"
     CDS             complement(<1..929)
                     /locus_tag="VN496_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXF17284.1"
                     /translation="MDYYDDLEIRDPIQRERALFNALPGHISHAKANTAYFARLLAGI
                     DAGAIRDRQALAGLPVTRKSDLIALQKEAPPFAGMTALSVGQLGRVFASPGPIYDPQG
                     GRGDAWRFGRALFAAGFRRGDLVHNTYSYHFTPAGFMIDLAAQAIGCAVFPAGTGQTE
                     LQIAAVADLKPSCYAGTPSFLKILLEKADELKADVSSLVKASVSGEALLPPVRQWIER
                     RGITVRQCYGTADLGLIAYETEPMGGLVVDEGVIVEIVRPGTGDPVAAGDVGEVVVTS
                     LCMEYPLIRFATGDLSAVAPGVSACGRTNMRIKG"
     gene            complement(986..1798)
                     /locus_tag="VN496_09790"
     CDS             complement(986..1798)
                     /locus_tag="VN496_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17285.1"
                     /translation="MNTQAVLLAVNNVEVIYDHVILVLKGVSLEVREGTIVALLGANG
                     AGKTTTLKAVSNLLASERGDVTKGSIELRGQRIDRLTPWEVVKRGVVQVMEGRHCFQH
                     LTVEENLLTGAFTRSANRAAIRRDLEMVYQYFPRLKERRRSQAGYTSGGEQQMTALGR
                     ALMAKPGMILLDEPSMGLAPQIVDEIFQIVRGLNENEKVSFLLAEQNTNVALRHADYG
                     YILENGRVVMNGPAADLAGNEDVKEFYLGVSTTGRKNFRDAKHYRRRKRWLA"
     gene            complement(1912..3234)
                     /locus_tag="VN496_09795"
     CDS             complement(1912..3234)
                     /locus_tag="VN496_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17286.1"
                     /translation="MRTWTKVAISLIALAGGTVTVLPVAAQQAGSQFMGILGYRVGPY
                     GANGAAFFTGFLDYLALVNERDGGINGVKLSWEECETEYNNAKGVECYERLKTRSESG
                     PTAIHPMSTGITYGLIDKAPVDKIPLITLGYGRTDATDGRVFPWVFPLLSNYWDAATG
                     IVKFIADKEGGSEKLKGKKIVLLYHDSAYGKEPHPVLEAEAAKLGFELKLIPVPHPGN
                     EQQSQWLQIRQYKPDWVILWGWGVMSATALSTAQKVGFPRDKMVGNWWAGSEIDTEAA
                     GPASEGYYAASLNLAGKDFGVVKDVEKFVYGKGKGGDPKLVGTVLWNRGLAAAMYTVE
                     AVRTAQAKFGKKPMTGEQTRWGIENLNVDQKRLDQLGFGTMVPPLKLSCSDHGGSGLV
                     RFQKWEGGKWKPASEWMSGDKALVRKMVEDSAAKYATEKSITAGCMKS"
     gene            complement(3263..4339)
                     /locus_tag="VN496_09800"
     CDS             complement(3263..4339)
                     /locus_tag="VN496_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17287.1"
                     /translation="MFYRETGQFKTTYAADQALFPIRQDRHAMVAMLVVAFIGIPLLS
                     SDYWLTALLIPFLVFSLAALGLNILTGYAGQLSLGTAAFMAVGAIAAYNFQLRIPGMP
                     ILLSFLCGGLSAAVIGMLFGLPSLRIKGFYLAVATLAAQFFIPWLVTKVGWFSNHSAS
                     GVITAQPIVILGVAFDSPQKKYLLVVFIVALLALLAKNLTRSRIGRAWMAVRDMDVAA
                     EVIGIRILRTKLTAFAVSSFYCGVAGALYAFAYLGSVEPDGFNLDLSFRILFMVIVGG
                     VGSILGSFLGAAFITLMPIVIEVLLTRAGHAFNLTLEPGVTSMVQLLVFGGLIIFFLI
                     VEPHGLARLWQITKEKLRLWPFPY"
     gene            complement(4414..5310)
                     /locus_tag="VN496_09805"
     CDS             complement(4414..5310)
                     /locus_tag="VN496_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007246480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17288.1"
                     /translation="MSFFFEVLIGGLLAGVMYSLVALGFVLIYKASGVFNFAQGAMVF
                     FAALTFVGLQEKFSLPFWAAFMLSLAAMVVLGTLIERIVLRPLVNQPQITLFMATIGL
                     SFFIEGLAQLIWGTNVRRLDIGIKDEPIPAVLQHFDMAISSFDLTAALVAGILVTVLA
                     VFFQVTRVGRALRAVADDHQAALAIGIPLKQIWAIVWSVAGFVALVAGLMWGARNGVQ
                     YALTFVALKALPVLILGGFESVLGAIVGGLIIGALEKLAEVYLGPHIGGGIEGWFAYM
                     MALLFLLVRPEGLFGEKRIDRV"
     gene            complement(5372..6160)
                     /locus_tag="VN496_09810"
     CDS             complement(5372..6160)
                     /locus_tag="VN496_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17289.1"
                     /translation="MADAIKAGGVLLAVENIALAFGGVRALSDVTFEVREGEIRAIIG
                     PNGAGKSSMLNVINGVYHPQQGTVRFRGEIRRSMDPHFAASHGIARTFQNIALFKGMS
                     VLDNIMTGRNLKMHAGLLEQAFYIGRNRREELAHRAKVEEIIDFLQIEHIRKTPVGRL
                     PYGLQKRVELARALAAEPALLLLDEPMAGMNVEEKQDMCRFILDVNDEFSTTIVLIEH
                     DMGVVMDICDRIVVLDYGRKIGDGTPDEIRSNKGVIDAYLGLSH"
     gene            complement(6206..8170)
                     /locus_tag="VN496_09815"
     CDS             complement(6206..8170)
                     /locus_tag="VN496_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXF17290.1"
                     /translation="MKGPDTRDAPASQNFPALLLHHAANQGDRTAIREKDLGIWQSWT
                     WEQVADEVRALACGLAAQGFRRGMNLAIIGDNRPRLYWSFAAAQCLGGVPVPMYQDAV
                     AQEMLYVLDNAEIQYAVVEDQEQVDKLLELLPKYPNLKHIFYDDPRGLRHYAQPQLMP
                     YDRLRELGVEYNRDHPGFFDGAVGTVQPDDVCVMLYTSGTTSKPKGVCLTHAALIAAA
                     RGDVAFDGFGPSDEILSYLPMAWVGDHLFSYAQALVAGFTVNCPESSETAMNDLREIG
                     PTYYFAPPRIFENLLTTVMIRMEDASRIKRGLFHYFMAIARRCGAELMDGKPVPLSDR
                     MLYRLGDLLIYAPLRNSLGMSRLRVAYTGGAAIGPDLYRFYRSIGINLKQLYGQTETA
                     AYVCKQPNGEAKLDTVGKPMPGVEVRITGKGEIWVKTPSMLKEYYKRSDASAESISPD
                     GYFMTGDAGFFDKDGHLKIIDRANDVGKLANGALFAPQFIENKLKFYSYVKEAVCFGD
                     RREMVCAFINIDLQAVGNWAEKRNLAYSGYLDLTRRTEVYELILACIEQVNADLARES
                     ELASSQVRRFLILHKELDADDDELTRTRKVRRGFVAEKYAPLIDALFSGKTGCHIATE
                     VKFEDGRRGTIEADIKIQDVMASEPGREAA"
     gene            complement(8305..8628)
                     /locus_tag="VN496_09820"
     CDS             complement(8305..8628)
                     /locus_tag="VN496_09820"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF17291.1"
                     /translation="MSDFQLNHLNIPARDPEGLVRWYASTFGLQADGNKVRGPGVLIA
                     FQAGEPVKRAPELHLGFQVPNNQKLADWASKFGATVKRGAEFNAFRTFDPEENCLEIY
                     CKAES"
     gene            complement(8646..>10253)
                     /locus_tag="VN496_09825"
     CDS             complement(8646..>10253)
                     /locus_tag="VN496_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXF17292.1"
                     /translation="FEDDGEMLKWLMRENLPGYFPYTAGVFAFKRENEDPTRMFAGEG
                     DPFRTNARFKFLSRDMAAKRLSTAFDSVTLYGFDPHERPDIYGKVGNSGVSIATLDDM
                     KVLYSGFDLCDPATSVSMTINGPAPTILAMFVNTAIDQQIDKFIEQHGRAPNEQETAK
                     IRIWSQENVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIQEYFVKHKVRNFYSVSI
                     SGYHIAEAGANPISQLAFTLSNGFTFVEAYLARGMHIDDFAPNLSFFFSNGMDPEYAV
                     LGRVARRIWATTMRDKYGANERSQKLKYHVQTSGRSLHAQEIAFNDIRTTLQALIAIY
                     DNCNSLHTNAYDEAITTPTAESVRRALAIQLIINREWGLAKNENPNQGAFIIDELTDL
                     VEEAVLQEFERIAERGGVLGAMETGYQRGKIQEESMYYEHKKHDGSYPIIGVNTFRNA
                     EATQTGGSIPLQRSTDEEKQSQIRRLREFQARHADQAPAALARLRQTVIDNGNVFAEL
                     VNAVRVCSLGQITDALFEVGGQYRRSM"
BASE COUNT         2190 a         3081 c         2804 g         2178 t
ORIGIN      
        1 cccttgatgc gcatattggt gcgtccgcac gcactaacgc ctggcgcgac agccgacaaa
       61 tctccggtag caaagcggat cagcggatac tccatgcaca aacttgtgac cactacttcg
      121 cccacgtctc ctgcagccac cgggtcgccg gtgcccggcc gcacgatttc tacaatgacg
      181 ccttcgtcga ccaccagccc gcccatgggt tcggtctcat acgcaatgag cccaaggtca
      241 gcagtcccgt agcattggcg aaccgtaatg ccgcgacgct cgatccattg ccgcaccggc
      301 ggcaacaaag cctcacccga cacgcttgcc ttaacaaggc tgctgacatc ggctttcaac
      361 tcatcggcct tttccagaag tatcttcagg aaagatggcg tgccggcata gcagctcggt
      421 ttgagatccg caacagcagc gatctgcaat tccgtctgtc cggtgccggc cggaaacacc
      481 gcgcatccga tcgcctgggc agcaaggtcg atcatgaacc cggcaggcgt aaagtgatac
      541 gaatacgtgt tgtggaccag atctccgcgc cggaaccccg cagcaaacag cgcgcgtccg
      601 aagcgccatg cgtcaccgcg cccaccctgc gggtcgtaga tggggccggg ggaggcgaac
      661 accctgccaa gttgcccaac ggaaagcgca gtcattccag caaacggcgg agcctccttc
      721 tgtagtgcaa tcaggtcgga tttgcgcgtg acgggaagtc cggcaagcgc ctggcgatcg
      781 cgaatggcgc ccgcatcgat cccggccagc aagcgcgcaa aatatgcggt gttggctttg
      841 gcatggctta tgtgacctgg taatgcattg aacaacgcgc gctcacgctg tatcgggtcg
      901 cggatctcaa ggtcgtcgta gtagtccatc agggagcggc ggccactatc aaattaaagt
      961 tctgttccga gccacggacg cacgctcagg caagccatct tttgcggcgg cgatagtgct
     1021 tggcatcgcg gaaattttta cgcccggtag tggacacgcc cagatagaac tccttcacgt
     1081 cctcgttgcc ggccaggtcg gcggccggtc cattcatgac gacgcgaccg ttttccagga
     1141 tgtagccgta atcggcatgg cgcagggcaa cgttggtatt ctgctcggca agcagaaagc
     1201 ttactttttc gttttcattg agcccgcgca cgatctgaaa aatttcgtcc acgatctgcg
     1261 gcgccagccc cattgaaggt tcatcgagca ggatcatgcc gggcttcgcc atcagcgcgc
     1321 ggcccagcgc ggtcatttgc tgttccccgc cggaggtata gcctgcctgg ctcctgcgcc
     1381 gttctttcaa acgcggaaag tactgataaa ccatttccag gtcgcgccgg attgctgcac
     1441 ggtttgcgct gcgcgtgaag gcgccggtca gcagattttc ttcaacagtc agatgttgga
     1501 agcaatgtct gccttccatg acttgcacca cgccgcgttt cacgacttcc cacggcgtga
     1561 ggcggtcgat gcgctggccg cgcaattcaa tcgagccctt ggttacatcg ccacgttccg
     1621 acgcgagcag atttgaaact gccttcagcg tagtggtctt gccggcgccg ttcgcaccca
     1681 gcagcgccac aatggtccct tcccggactt ccagcgaaac gcccttgagg accaggatga
     1741 catggtcgta gatgacttcg acgttgttga cagcaagcag caccgcctgt gtattcatac
     1801 tgcgtgaagc ctgttggttg atgaagggtg aatgttgtga agggccgctg ccgcaagggc
     1861 gcagtcaact catccttcac cacatttacg gtttagaaca ggagaaacga ttcagctctt
     1921 catgcagccc gccgtaatgc tcttttcggt agcgtacttg gcagcggaat cctctaccat
     1981 cttgcgcacc agcgccttgt ccccgctcat ccattcagac gctggtttcc atttaccgcc
     2041 ttcccacttc tggaatcgca ccagaccgct gccgccgtga tccgagcagg acagttttag
     2101 cggaggaacc atggtgccga agcccagctg gtccaggcgc ttctggtcga cgttgagatt
     2161 ttcgatcccc cagcgcgtct gttcgccggt catgggcttc ttgccgaact ttgcctgcgc
     2221 agtacggact gcttcgacgg tatacattgc tgctgccagg ccacggttcc acagcactgt
     2281 gcccaccagt ttcggatcgc cgcctttgcc cttcccgtaa acaaacttct caacgtcctt
     2341 gacgacgcca aaatccttgc cggcaagatt gaggctggcc gcgtaataac cttcggacgc
     2401 ggggcccgct gcctcggtat caatctctga acccgcccac cagttgccaa ccattttgtc
     2461 gcgcgggaag ccgacctttt gcgccgtcga caatgccgtt gcactcatta caccccagcc
     2521 ccacaagatt acccagtccg gtttgtactg ccgtatctgc agccattgtg attgctgttc
     2581 gttgcccgga tggggtaccg gaataagttt cagctcaaaa cccagtttgg cggcctccgc
     2641 ttcaagaacc gggtgaggtt ccttcccata agcagaatcg tgatagagca gaacgatctt
     2701 cttgcccttg agcttctcgc tgccgccttc cttgtccgct atgaacttca cgataccggt
     2761 agcggcgtcc cagtaattgg atagcagcgg gaatacccac ggaaaaacgc ggccgtcggt
     2821 tgcgtccgtc cttccgtacc ccagagtgat cagcggaatc ttgtcgacgg gtgccttgtc
     2881 gatgagaccg taggtaatcc cggtggacat gggatggatc gccgtcggac cgctttcgct
     2941 acgggtcttg agtctttcat agcactccac gcctttagca ttgttgtact cggtttcgca
     3001 ttcctcccat gacagcttca cgccatttat gccgccgtca cgctcattga ccagcgccag
     3061 ataatcgaga aagccggtga agaaagcggc gccgttcgca ccataggggc cgacgcggta
     3121 tcccaggatt cccataaact ggctcccggc ctgctgcgct gctaccggca gcacagtaac
     3181 ggtgccacca gccagcgcta tcagcgagat tgctactttt gtccaggttc tcattgtgtt
     3241 cctccagatg tgttctgctg tgctagtacg gaaaaggcca aagccgcaat ttttcttttg
     3301 taatttgcca caaacgcgcc agtccgtgcg gttcaacaat caggaaaaaa atgatcagcc
     3361 caccgaacac caacagctgc accattgacg taacgcccgg ttccagggtc aggttgaacg
     3421 catggcctgc tcgcgtcaac agcacctcga tcacgatagg catcagcgta atgaaggccg
     3481 ctcccaggaa agagcccagg atgctgccca cgccaccgac gatcaccatg aacaggatcc
     3541 ggaaagaaag gtccaggttg aagccgtccg gctccacgct gcccaaatac gcaaacgcat
     3601 agagtgcgcc ggcaacgccg cagtaaaacg aactcactgc aaatgcagtc agtttggtgc
     3661 gcaagatgcg aatgccgatc acctcggccg cgacgtccat gtcgcgtact gccatccacg
     3721 cgcggccaat gcggctgcgg gtcagatttt tcgcgagcag ggcgagcagc gcaacgataa
     3781 agacaaccaa cagatatttc ttctgcggcg aatcaaaggc cacgcccagg atcacaatcg
     3841 gctgcgcagt gatcactccc gaggcggaat gattcgagaa ccagccgact ttggtgacca
     3901 gccagggaat gaaaaactgc gccgcgagcg tagccacggc aagataaaag cccttgatgc
     3961 gcaggctggg gagaccgaac agcatgccga tcaccgcagc cgacaaaccg ccacacagaa
     4021 aactcaacag gatcggcatg cccggaatac gtaactggaa gttgtaggcg gcaattgcgc
     4081 ccaccgccat aaaggccgct gtccccaacg acaactgacc cgcatagcca gtcaggatat
     4141 tcaagccgag cgcggccagg gaaaacacca gaaacggaat cagcagcgca gtcagccagt
     4201 aatcgctgga gagcaacggt atgccgatga aggcgacaac cagcatggca accattgcat
     4261 ggcgatcctg ccgaatcgga aacaatgcct gatcggcggc gtatgtggtt ttgaattggc
     4321 ctgtttcgcg gtagaacatt tcagtgtttg atggttgagc ttcagttcat ggcaaggcgc
     4381 gtttcggtgc aagcttcctc tcgatgtaga acgctatacc cgatcaattc ttttttctcc
     4441 aaacaagccc tccggtcgca cgagcaggaa caacaacgcc atcatgtagg cgaaccaccc
     4501 ctcgatgccg ccaccaatgt gtggcccgag atacacctcg gcaagtttct ccagtgcacc
     4561 gatgatcagc ccgccgacga tggcgccgag tacggattcg aagccgccca ggataagcac
     4621 cggcaaggcc ttcaacgcta caaaagtcaa cgcgtattgc acgccgttgc gcgcacccca
     4681 catcaggcca gcgaccagcg ccacgaaccc ggcaaccgac catacgatgg cccatatttg
     4741 cttcagcgga atgccaatgg cgagcgcggc ctgatggtca tcagcgaccg cgcgcaatgc
     4801 ccggcctacc cgggtaacct gaaagaacac agccagcacc gtgaccaata tgccggccac
     4861 cagcgcagcg gtcagatcaa agctgctgat cgccatgtcg aaatgctgca gcaccgccgg
     4921 gatgggctcg tccttgatgc cgatatcgag ccggcgcaca ttagtgcccc agatgagttg
     4981 cgcgaggcct tcaatgaaaa acgacaagcc gatggtcgcc atgaacaacg taatctgggg
     5041 ttgattcact agcgggcgca acacgatacg ttcaatgagc gttcccagca caaccatggc
     5101 cgctaatgac agcatgaatg cggcccagaa cggcagggag aatttttcct gcaggccgac
     5161 gaacgtcagc gctgcgaaga acaccatcgc gccttgggca aagttgaaca cgccggacgc
     5221 cttgtagatc agcacgaatc caagcgcgac cagcgagtac atgactcccg cgagtaaccc
     5281 gcctatcagc acctcgaaga aaaagctcat tcccggaatc ctgaatcagc ctgcccgctg
     5341 cgcgtttgcg ctttatctct atccggcgca ctcaatgtga tagacccaaa taagcatcga
     5401 tgactccttt gttgctgcgg atctcgtccg gagtgccatc gccgatcttg cggccgtagt
     5461 cgagcacgac gatgcggtcg cagatatcca tcaccacgcc catgtcatgc tcgatcagca
     5521 cgatggtggt gctgaattcg tcattgacgt ccaggatgaa acggcacatg tcctgttttt
     5581 cttcaacatt catccccgcc atcggctcat cgagcagcaa cagtgccggt tcggctgcca
     5641 gtgcgcgagc cagttcgaca cgtttctgca agccatacgg caaccggccg accggtgtct
     5701 tgcgaatatg ttcgatttgc aggaagtcga tgatctcctc gactttcgcg cgatgcgcca
     5761 actcttcccg gcgattgcgt ccgatataaa aagcttgctc cagcaggccc gcatgcatct
     5821 tcagattgcg accggtcatg atgttgtcca gcacggacat gcccttgaac agcgcgatgt
     5881 tctggaaagt gcgcgcgata ccgtgcgatg cggcgaaatg cgggtccatg ctgcggcgta
     5941 tttcaccgcg gaagcgaacc gtgccttgct gcggatgata aacgccattg atgacgttca
     6001 gcatcgaact tttgccggcg ccattggggc cgatgatcgc gcgaatctcg ccttcgcgaa
     6061 cctcgaaagt cacatcggag agcgcgcgca cgccgccgaa ggcaagggct atgttttcga
     6121 ccgccagcaa aaccccgcct gctttgattg catccgccat gattgttgtt gttgtcgcct
     6181 gtatggcagc atccggaata gatagctatg cggcttcccg tcccggttcc gaggccatta
     6241 cgtcctggat cttgatatcg gcttctatcg tgccacggcg gccgtcctca aacttcacct
     6301 cggtggcgat atggcaacct gttttccctg agaacaaagc atcgatcagc ggggcatatt
     6361 tttcagccac aaatccccga cgcactttgc gcgtgcgcgt tagctcatca tcgtccgcgt
     6421 cgagttcctt gtgcaggatc aaaaaacgcc gcacttgcga actcgccaat tctgattcgc
     6481 gcgccaggtc cgcattgacc tgctcgatgc aggccaggat caactcatat acttccgtgc
     6541 gcctcgtcag atcgagatag ccgctgtaag ccagattgcg tttctccgcc caattgccga
     6601 cggcttgcaa gtcaatgttg atgaaggcgc aaaccatttc cctacgatca ccgaagcaaa
     6661 ccgcttcctt gacataggag tagaacttta gcttgttctc gatgaactgc ggtgcaaata
     6721 gcgcgccgtt agcgagcttg ccaacgtcat tggcgcggtc aatgatcttg aggtggccgt
     6781 ctttgtcgaa aaaaccggca tcaccggtca tgaagtagcc gtctggcgaa atcgattccg
     6841 cgcttgcatc gctgcgcttg tagtattcct tgagcatgct gggcgtcttg acccagattt
     6901 cgcctttgcc ggtgatccga acttcgacgc cgggcattgg cttgccgacc gtatcgagct
     6961 tcgcttcacc gtttggctgt ttgcagacgt aggcggctgt ttccgtctgg ccgtacagct
     7021 gtttcagatt aatgccgatt gagcggtaga agcggtacag atctggtccg atagcggcac
     7081 cacccgtgta cgcgacccgc agccggctca tgccgaggct gttgcgcagt ggggcgtaga
     7141 tcagcaaatc gcccaagcgg taaagcatcc gatcggataa tggaaccggc ttgccgtcca
     7201 tcagttcggc accgcaacgg cgcgcgatgg ccatgaaata gtggaacagg ccgcgcttga
     7261 tgcgcgaggc atcttccatg cgaatcatga ccgtagtcag caggttctca aaaatgcgcg
     7321 gcggtgcgaa gtaataggtg gggccgattt cgcgcagatc gttcatcgcg gtttccgacg
     7381 attcgggaca gttgacggtg aatcccgcca ccagcgcctg tgcataagaa aacaagtgat
     7441 caccgaccca ggccatgggt agataggaca gaatctcgtc ggaagggcca aagccatcga
     7501 atgcgacatc cccgcgcgca gcggcaatca gcgcagcatg ggtcaggcaa acgcctttgg
     7561 gcttgctggt cgtgccggac gtgtacagca tgacgcagac atcgtccggt tggacagtgc
     7621 ccacggcacc gtcaaagaag ccgggatgat cgcgattgta ttccacaccg agctcgcgca
     7681 gccggtcata aggcatcaac tgtggctgcg cataatggcg caatccacgc ggatcatcgt
     7741 aaaagatatg cttcagattg ggatacttcg gcaggagctc gagcaacttg tcgacctgct
     7801 cctgatcctc gacgaccgcg tactggattt cggcgttgtc gagcacgtag agcatttcct
     7861 gcgcaactgc gtcctggtac atcggcaccg gcacgccacc caggcattgg gctgccgcga
     7921 atgaccagta caaccgcggc cggttgtcgc cgatgatggc aaggttcatt ccgcgccgga
     7981 agccttgtgc ggccagcccg caggcaagag cacgcacctc gtcggcgacc tgttcccagg
     8041 tccagctctg ccagatgccg agatccttct cgcggatggc ggtacggtcg ccctggttgg
     8101 ctgcatgatg caacaacagc gccgggaaat tctggcttgc gggtgcatcg cgcgtgtctg
     8161 ggcccttcaa tctagctccc ccgcaaattg gcacactatc ggtgtcatga ctgcgtcagt
     8221 acttaagctt cggccggcag gcgacgccgc atcgagttgc ggggacactg ccagcgttcc
     8281 cgttttttct attcaatctt ttcgtcagct ttcggctttg cagtaaatct ccaggcaatt
     8341 ttcttccgga tcgaacgtgc ggaatgcgtt gaactctgcg ccccgcttga ccgtggcgcc
     8401 aaatttgctg gcccagtccg cgagtttttg attgttcggt acctggaacc ccaaatgaag
     8461 ttcgggcgca cgtttcaccg gctcacccgc ctggaacgcg atcagcacgc ccgggccgcg
     8521 caccttgttg ccgtcggctt gcaaaccgaa cgtgctcgca taccagcgca ccagaccctc
     8581 gggatcgcgc gcagggatgt tcaagtggtt taattgaaaa tcagacatct cgtctccctc
     8641 gagcactaca tactgcggcg gtactggcct cccacctcga acagcgcgtc agtgatctgt
     8701 cccagcgagc agacgcgtac cgcattgacc agttcggcaa acacattgcc attgtcgatg
     8761 acggtctggc gcagccgcgc cagcgcagcg ggcgcctggt ccgcgtggcg cgcctgaaat
     8821 tcgcgcaagc gacggatctg gctttgcttc tcctcgtccg tcgagcgctg caaaggaatg
     8881 cttccgcccg tctgcgtagc ctccgcattg cggaacgtgt tcactccgat gatcggatag
     8941 gagccgtcgt gctttttgtg ctcatagtac atcgactctt cctggatctt gccacgctgg
     9001 taaccggttt ccattgcgcc cagcacgccg cctcgctcag cgatgcgttc gaactcctgc
     9061 aagactgctt cctccaccaa atccgtcagt tcgtcaatga tgaacgcgcc ctggtttgga
     9121 ttctcgtttt tcgccaagcc ccattcccgg ttgatgatca attgaatggc gagtgcgcgc
     9181 cgcacggatt ccgcggtcgg agtcgtaatg gcttcatcgt aggcgttggt gtgaagcgaa
     9241 ttgcaattgt cgtagatcgc gatcagagcc tgcagggtgg tgcgaatgtc gttgaaggcg
     9301 atttcctgcg cgtgcaaaga gcgcccggac gtctgcacat ggtatttgag cttctggctg
     9361 cgttcattgg cgccgtattt gtctctcatc gtggtcgccc aaatgcgccg ggccacgcgt
     9421 cctagcacgg catattctgg atccatgccg ttggagaaaa agaaagaaag attgggcgcg
     9481 aagtcgtcga tatgcattcc gcgcgccaga taggcttcga cgaaggtgaa gccatttgac
     9541 agcgtgaagg cgagctggga aatcggattt gcaccggcct cggcgatgtg gtagcccgaa
     9601 atcgataccg aatagaaatt gcgcaccttg tgcttgacga aatattcctg gatatctccc
     9661 atgaccttga gcgaaaactc ggtggagaaa atgcatgtgt tctggccctg gtcttccttc
     9721 agaatatcgg cctgcactgt cccgcgtacg ttttcctgtg accaaattcg gattttcgcc
     9781 gtctcctgct cgttcggtgc tcgcccatgc tgctcaatga acttgtcgat ctgctggtct
     9841 atcgcggtat tgacgaacat cgccaggata gttggagcgg gcccattgat ggtcatggag
     9901 acggaagtag cggggtcgca cagatcgaag cccgaataca gcactttcat gtcatccagc
     9961 gtagcgatcg agactccaga attgccgact ttgccataga tgtccggtcg ctcgtgcggg
    10021 tcaaatccgt acagcgttac cgaatcaaac gccgttgaaa gtcttttggc cgccatgtcg
    10081 cgggacaaaa acttgaagcg cgcattggtg cggaaaggat cgccttctcc ggcgaacatg
    10141 cgggtcggat cttcgttttc gcgtttgaac gcaaatacgc cagccgtata cggaaaataa
    10201 cccggcagat tttcccgcat cagccatttc agcatttcgc cgtcatcttc gaa
//
LOCUS       DATMZQ010000301         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4046992, whole genome shotgun sequence.
ACCESSION   DATMZQ010000301 DATMZQ010000000
VERSION     DATMZQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4046992"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..976)
                     /locus_tag="VN496_09830"
     CDS             complement(5..976)
                     /locus_tag="VN496_09830"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXF17293.1"
                     /translation="MNADRLKSKLEGCYVTIPTPFRDEPGYPINEQALRSYVRFLLGG
                     GLKDNYATLLAAGAAGDFSTMSFKERIAVAEIVIEEAKGKVPLAFGGQTTSTLELIQL
                     ARAAQSLGFDFLQVSCPYYFTHTEADFEEFVREAAAAAPELGIIVYNTFWTATNLTSS
                     LIARLADVPNIVGLKWATPRTDAMEFEDISSNYSRRFTIIDNNLYFPYSAMPSLGARA
                     FEVHLCNYWPEWGIRLVDQVKAGNYAEIARMMVKEAMPFYKLWVRIEKEYTSGDGYLD
                     KLCMELVGLPSSRCRPPTRDVRGRYRNAAAEMLKATGVPRFERLQSA"
     gene            1122..2141
                     /locus_tag="VN496_09835"
     CDS             1122..2141
                     /locus_tag="VN496_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HXF17294.1"
                     /translation="MRIRRSSDRAPRFVFLLQAEFPANAFILASEALRIANQNSGRWL
                     FETVVLSETGQPVRASNGMWITPDGDIGSIQQAEYVLLFGGNLPTQHNSPRLLAALRT
                     ARRFGSTVIGVDTGTFALAQAGLVKSKKVTLHWEAMPAYTERFPESVVEDCLHMFDGN
                     IGCCAGGIATLDLMLKLIEDLRGRALADEVANALVHTRRDAEQAQRPADSAKQVGPSF
                     SRRLISLMEENLDFPLATGRIAERLGVSTRTLERQCNRHFGQAPAALYLKVRLQAARN
                     LLFYEDLSVKTISLACGFSYPSVFSRAFMQQFGQSPRSFRAAVRAAQDQTLRPELHRL
                     TSSQL"
BASE COUNT          521 a          696 c          684 g          538 t
ORIGIN      
        1 tcgatcatgc gctctgaagc ctttcgaagc gtggaacgcc ggtggccttg agcatttctg
       61 ccgcggcatt gcggtagcgg ccccttacgt cgcgggtcgg aggacggcac cggctggatg
      121 gcaggccgac aagttccatg caaagcttgt cgagatagcc atcgccgctg gtatactcct
      181 tctcgatccg gacccatagc ttgtaaaagg gcattgcctc tttcaccatc atgcgggcga
      241 tctcggcgta attacctgcc ttgacctgat cgacgagccg aattccccat tccggccagt
      301 aattgcacaa gtggacctcg aaggcgcggg ccccgagcga gggcatcgcc gaatagggaa
      361 agtacaagtt attgtcgatg atggtgaagc gacgtgaata gttcgatgag atatcctcga
      421 attccatggc gtcggtgcgc ggcgttgccc atttcagacc gacgatgttc ggtacatccg
      481 ccaggcgcgc tatcaggctc gaggtgagat tcgtcgccgt ccagaaggtg ttgtagacaa
      541 tgattccgag ttcgggtgct gctgcagcag cttcgcgcac gaactcctcg aaatccgctt
      601 cggtgtgggt aaagtagtac gggcacgaca cctgcaggaa atcgaagccc aagctttgcg
      661 cggcgcgtgc cagttggatc agctcaagag tgctcgtcgt ctggccgccg aacgcgagcg
      721 gcacctttcc cttggcttcc tcgatgacga tttcggcaac ggctatcctc tccttgaaac
      781 tcatggtgga gaaatcgcct gcagcgccgg cggccagcag cgttgcgtaa ttgtccttca
      841 atccgccgcc cagcaagaac cgcacgtagc tgcgcagggc ctgttcgttt atcgggtagc
      901 ccggttcgtc ccgaaatggc gttgggattg ttacgtagca gccttcgagc ttcgatttca
      961 gtcgatcggc gttcaagcca atactccaac ttttccggcg cgtttagcgc ggcggacccg
     1021 caatttcgat gtgcttagtc acgtgttgag ccttctatat ttgaccaaat aactgcggta
     1081 tattgacttt ctgcgccatt tacttagcat caagcgtcac aatgcgaata cgtcggtcca
     1141 gcgacagagc cccacggttt gtgttcctgc tgcaagcgga atttcccgcc aacgctttca
     1201 tcctcgcgag cgaggcgctg cgaattgcca atcagaacag cgggcgctgg ctattcgaaa
     1261 cggtggtctt gtcggaaacg ggccagcccg tgcgggccag caacggcatg tggatcacac
     1321 ccgacggcga catcggcagc attcagcaag cagaatacgt gttgctgttt gggggaaatc
     1381 ttccaaccca gcacaattcg ccgcgtctgc tggccgcttt acgaacagcc cggcgcttcg
     1441 gctcgactgt tatcggcgtc gataccggga ctttcgctct cgcgcaggcg ggtctggtga
     1501 agtcgaagaa ggtgacgctg cattgggagg ccatgccggc ttacaccgaa cgcttccccg
     1561 aaagcgtggt tgaggattgc ctccatatgt tcgatggaaa tatcggctgt tgcgccggcg
     1621 gcatcgccac gctcgatctt atgttgaaac taatcgaaga cctgcggggc cgagccttgg
     1681 cggatgaagt cgcaaatgcg cttgtgcata cgcgtcgcga tgcagagcag gcgcagagac
     1741 cagccgattc cgccaaacag gtcggcccca gcttttcacg ccggctcata tccttgatgg
     1801 aggaaaatct cgattttccg cttgccaccg gacgaattgc cgaacggctg ggtgtgtcca
     1861 caagaacgct ggagcgacaa tgcaaccggc acttcggtca agcgccggca gcactttatc
     1921 tgaaggtccg cctgcaagct gcacgcaacc tgcttttcta cgaagatctc agcgtgaaga
     1981 cgatttcgct cgcctgcgga ttttcctacc cttccgtatt cagccgcgca ttcatgcagc
     2041 agtttggtca gtctccgcga agctttcgcg cagcggttcg cgctgcccag gaccagacgc
     2101 tacgtcccga gctccaccgc ctgaccagct cgcaactatg acctgcccca tttgtttctg
     2161 tatacgcacg atcataaagg taaatcctaa aaacatcggg ttttgcatgc tcaattgacg
     2221 ctgcgcagca acaccctcta acctgcacgt ttgggaagca aagatgattc ggatctagaa
     2281 cacatcaaac ggatcaggca tcccataggc aaatgaggag aggggtatgg gcaataagca
     2341 gaccgacgat gtaaaagtcc ttcatgaaat gggatacgcg caagagctga cgcggcgcat
     2401 gggggcattt caaaacttcg caatatcatt tgccattat
//
LOCUS       DATMZQ010000302         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3578814, whole genome shotgun sequence.
ACCESSION   DATMZQ010000302 DATMZQ010000000
VERSION     DATMZQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3578814"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VN496_09840"
     CDS             complement(<1..1061)
                     /locus_tag="VN496_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17295.1"
                     /translation="MTKVCTTVAAVITFSLCGAVWAVPPYRYQISLDESLEQLAVHAC
                     FDGRVPEALVAGSDDARFYLQSMRLGEHALEPVGDKVMLGNAGENACIDYVVKLQPAQ
                     SLAQSGGPETRRIGSHDMLTAIGDWLWRPLDTAAQIELRFRLPAGIKVSAPWRSETGP
                     DGPPIFLAGDTPPGWPGVVVFGNFTSHEIHMADSVSNVALLDAPTQPERFEKWIEGAA
                     RNVASLYGRFPVASLQIVVSPTPRGRGPVPWAYVSRGGGPAVHLFINPARAPAEFERD
                     WSLTHEMAHLFLPYVDARDAWLFEGLPTYLQNVLMARGGAISVEEAWRRMHAGFERGA
                     RTAPDVSFARANERIGQGN"
     gene            complement(1058..1519)
                     /locus_tag="VN496_09845"
     CDS             complement(1058..1519)
                     /locus_tag="VN496_09845"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HXF17296.1"
                     /translation="MAGALTAYLHYLAMIALAALLILELTLCVQGLSEERVRLLVRLN
                     HIYLGAGLLALVSGLVYLIWFGKGPAFYLHNPVFYLKLALFAALGLTSIPPTLQYLRW
                     LHGAGAGRYTVADYQVVRMRRHILAELLLIAIIPLMASFTARGIGIQTVAQ"
     gene            complement(1589..>3565)
                     /locus_tag="VN496_09850"
     CDS             complement(1589..>3565)
                     /locus_tag="VN496_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HXF17297.1"
                     /translation="AIHTAFLFHAIKAGMTMGIVNAGQLGVYADIPKDLLERVEDVIF
                     NRRADATERMVEFAESYKGEARIAVEDLSWREGSVNERITHALVRGINTYIVADTEQA
                     RQQFPYPIQVIEGPLMEGMNVVGDLFGAGKMFLPQVVKSARVMKEAVAHLIPYIEAEK
                     LRSGTAGQSKGKIVIATVKGDVHDIGKNIVSVVLQCNNFDVVNLGVMVPAEKILKVAR
                     EENADIIGLSGLITPSLEEMAHVAREMERQGFRVPLLIGGATTSRVHTAVKIEPQYSG
                     PTVWVPDASRSVGVCTRLLSEELRDEYMQKMKDEAEKVRIQHKGKKGQGPQYPIAEAR
                     RHGLKTDWKSYAPPVPVLSGVKVFQDYPLAEIAKDIDWTPFFQTWELAGRYPAILQDP
                     VVGAAARSLFDDAQAMLANIIAEKWLGASAVIGLFPANSVGDDVELYTDESRQRIATT
                     FHFLRQQMVKPLDRPNQCLADLVAPKETGVPDYVGAFAVTAGIGIDERVAEYERKHDD
                     YNAILLKALADRLAEAFAEHMHRRVRREFWAYAPDEALSIAEVIDEQYRGIRPAPGYP
                     ACPDHTEKALLFSLLNAPAQTGITLTESFAMLPTAAVSGFYFSHAQAQYFAVAKIARD
                     QVEDYARRKGASLQETERWLAPNLAYEPDAKLAA"
BASE COUNT          715 a         1124 c         1021 g          705 t
ORIGIN      
        1 atgttgccct gaccgatgcg ctcattcgcg cgcgcgaagc tgacatcagg cgccgtacgt
       61 gccccccgct cgaacccggc gtgcatgcgc cgccacgcct cttcaacgct gatcgcaccg
      121 ccccgcgcca tcaacacgtt ctgcaaataa gttggcagac cctcgaataa ccacgcatcg
      181 cgcgcatcga cgtagggtag gaacaagtgc gccatttcat gggtcaggct ccaatcgcgc
      241 tcgaactctg caggggcgcg cgccggattg atgaacaagt ggacggccgg gccaccgccg
      301 cgagaaacat aggcccacgg caccggaccc cggccacgtg gcgtagggga caccacgatc
      361 tgcagggaag ccaccggaaa gcggccatac agggaagcga cgttgcgcgc ggcaccttcg
      421 atccactttt cgaatcgttc cggttgcgtc ggcgcgtcga gcagagccac attcgaaacg
      481 ctatccgcca tgtggatctc atgggaagta aagttcccga aaacgaccac gccgggccag
      541 ccgggtggtg tatcgcctgc cagaaatatc ggcgggccgt caggcccagt ttcggatcgc
      601 caaggcgccg acacctttat gccggccggc aaccggaacc gcagttcaat ctgtgctgcc
      661 gtatcgagcg ggcgccacaa ccagtcaccg atcgcggtca gcatgtcatg cgatccgatt
      721 ctgcgagtct ccggacctcc cgactgcgcg aggctctgcg ccggttgtag cttcacgacg
      781 tagtcgatgc acgcattttc accagcgttg ccgagcatca ccttatcgcc gaccggttct
      841 agcgcgtgtt cgcccaatcg catcgactgc agatagaacc tggcatcgtc ggagccagcg
      901 accagcgctt ccggcacccg cccgtcgaag cacgcatgca ctgcgagttg ctccagcgat
      961 tcatcgaggc tgatctggta gcggtacggt ggcacagccc agaccgcgcc acagagggaa
     1021 aaggtgatta ccgcggcgac tgtggtgcaa acttttgtca ctgcgccacc gtctgaatgc
     1081 cgatgccacg cgccgtaaaa ctagccatca acggaatgat ggcgatcagg agcagctcgg
     1141 ccaaaatgtg acggcgcatc ctcaccacct ggtagtcggc caccgtgtat cgtccggctc
     1201 cagcgccgtg cagccagcga agatattgca gcgtgggagg aatcgaggtc aggcccagag
     1261 cggcgaagag cgcaagcttc agatagaaca ccggattgtg cagatagaaa gccggccctt
     1321 tgccaaacca tattaggtac accagcccgc tgacgagtgc cagcagtccg gcgcccagat
     1381 agatatggtt cagccgtacc agcagccgga cgcgttcctc gctcaggccc tgtacgcaca
     1441 gcgtaagctc caaaatcagc aaagcagcga gcgcgatcat cgccaggtag tgcaagtatg
     1501 ccgtcagagc gccggccaat tggtgctccc gcgcatagat accccgccac ccatgattgc
     1561 gttgcgcatg cgactggcac tcaagaattt atgccgcgag ctttgcgtcc ggctcgtagg
     1621 caagattggg ggccagccag cgctccgttt cctgcaggct tgcgcctttg cgtcgcgcgt
     1681 agtcctcgac ttggtcacgg gcgattttgg caaccgcgaa gtattgcgcc tgcgcatgcg
     1741 aaaaataaaa gccgctcact gccgcggtgg gcagcatggc aaaagactcc gtcagcgtaa
     1801 tgccggtttg agctggcgcg ttcagcaatg aaaacagcaa ggccttctcg gtgtggtcag
     1861 ggcaagccgg atagccaggc gctggccgga tgccacgata ctgctcgtct atgacctctg
     1921 cgatacttaa cgcttcgtca ggcgcgtaag cccagaattc ccgacgcacc cgccgatgca
     1981 tgtgttcggc aaaggcctcc gccagccggt cggcgagcgc cttgagcagg atcgcgttgt
     2041 agtcgtcgtg cttgcgttcg tattcggcca cgcgttcatc gatgccgatg cctgccgtta
     2101 cggcgaacgc tccgacataa tccggcactc cagtctcctt gggcgcgacc aaatcggcga
     2161 ggcactgatt tggccgatcc agcggtttga ccatctgctg gcgaagaaaa tggaaagtcg
     2221 tggcgatccg ttgccgcgac tcatcggtat agagttcgac gtcatcgccg acgctgttcg
     2281 ccgggaacag cccgatcacg gccgacgcgc caagccattt ttccgcgatg atattcgcca
     2341 gcatggcttg tgcatcatca aacaggctgc gtgcggcagc gccgacaacc ggatcctgca
     2401 agatggctgg gtatcgcccc gccagttccc aggtttggaa gaacggcgtc cagtcgatat
     2461 ctttggcgat ttccgcaagc ggataatctt ggaacacctt gactccggac agcacgggta
     2521 cgggaggcgc atagcttttc cagtcggtct tcagcccgtg acggcgcgct tcggcgatcg
     2581 gatattgcgg cccctggcct ttcttgcctt tgtgctggat gcgcactttc tccgcctcat
     2641 ctttcatctt ctgcatgtac tcatcgcgca attcttcgga cagcagcctg gtgcacacgc
     2701 cgacgctgcg tgaagcatcc gggacccata cggttgggcc ggaatactgc ggttcgattt
     2761 tcaccgcagt gtgcacgcgg gaggttgtcg ccccgccgat caacagggga acacggaaac
     2821 cctggcgctc catctcgcgc gccacatgcg ccatttcctc gagtgaaggc gtgatgagac
     2881 cggacaagcc gatgatatct gcattttctt cgcgagcaac cttcaggatt ttctccgccg
     2941 gtaccatcac gcccagattc accacatcga aattgttgca ctggagcacc accgacacga
     3001 tattcttgcc gatgtcgtgc acgtccccct tgaccgtggc aataacgatt ttgcccttgc
     3061 tctggccggc agtgccggag cgcagcttct ctgcttcgat gtagggaatc aggtgcgcga
     3121 ccgcttcttt catgacccgc gcggatttga cgacttgcgg caggaacatc ttgcccgcac
     3181 cgaacaaatc gccgacgaca ttcatgcctt ccatgagcgg gccttcaatc acctggattg
     3241 gatagggaaa ttgctggcgc gcctgttcgg tatccgcaac gatgtaggtg ttgatgcctc
     3301 tgaccagcgc gtgtgtgatg cgctcattca cgctgccctc ccgccaggac aggtcttcca
     3361 ccgcaattct ggcctcgccc ttgtagcttt ccgcaaactc aaccatgcgc tctgttgcgt
     3421 cggcccggcg attgaaaatc acatcttcca cacgctccag caggtccttc gggatgtccg
     3481 catagacacc gagttggcct gcattgacga tgcccatagt catgccggcc ttgatggcat
     3541 ggaacaggaa tgcagtgtgg atcgc
//
LOCUS       DATMZQ010000303         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1287005, whole genome shotgun sequence.
ACCESSION   DATMZQ010000303 DATMZQ010000000
VERSION     DATMZQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287005"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VN496_09855"
     CDS             complement(<1..1104)
                     /locus_tag="VN496_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HXF17298.1"
                     /translation="MKDRRKLRRMQRAAGLNAGQQGLIEHAGASVSVAASGGVWARFE
                     SLLEKPVSGASLAVFRIAVGLVMVLEAYALCTASPSTMGKSPLETFYTGPNVKFTFPY
                     AGFEWMPMLPARWIHLILGLQVAAGITVALGLLYRASAAALFLSWGYLYVVESTRTYW
                     MSHHYLELLVTFLMIWLPAAQRYSVDAWRTGRGKPPASVPYRAILLLRGQLVISYFYA
                     GVAKVNRDWLLDAEPVRYFLSKARAITDFAPHLTAPQLESLKHFLHSDGFAYFISYSG
                     ALFDLSVGFLLLIRRTRIFAMSLMVIFHATNHFIIFDDIGFFPLLGVTTALIFLDADW
                     PERFLRWLRRPRLSKPDWPWFVGGGIMFPVIGAS"
     gene            complement(1490..>2126)
                     /locus_tag="VN496_09860"
     CDS             complement(1490..>2126)
                     /locus_tag="VN496_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17299.1"
                     /translation="TSTGGKCKAAQDRGAVAVIFTTPTDLGTPFYAGATRTDLTIPVL
                     SIADVHAAKLKAALASGAVTGTIQGDSHFTVGGFDTGRGATDTIMSFIVPQAGVYPMR
                     MIFHQGGGGANCEWFSVKADGTKVLVNDVAGGGLKAFRTRTSVAGPRFNTPTRSGADI
                     IITWTGTGTLQQATAFTGNPATDWSDVTPTPVGNTYTVTAATSGNRFFRLR"
BASE COUNT          471 a          632 c          613 g          410 t
ORIGIN      
        1 cgacgcgcct attacgggga acatgatgcc gccgccgaca aaccacggcc aatccggctt
       61 cgataaccgc gggcgccgca accagcgcag gaaacgttcg ggccagtctg catcgagaaa
      121 gatcagcgcg gttgtaactc ccaacagtgg gaaaaaaccg atgtcgtcga aaatgatgaa
      181 atgattcgtc gcgtggaaga tcaccatcag ggacatcgca aagatcctcg tccgccggat
      241 caggagcaga aatccgacgc ttaaatcgaa cagcgcgcct gaataactga tgaaatacgc
      301 gaacccatca ctgtgcagga aatgtttcag ggattcgagt tggggagcgg tgagatgagg
      361 cgcgaaatcc gtgattgcgc gcgccttgga aagaaaatac cgcacgggtt ccgcgtccag
      421 cagccagtcc cggttgacct tggcgacacc ggcgtaaaaa tatgaaatca ccaactgccc
      481 gcgtaacaac aggattgccc ggtaggggac tgaagccggc ggctttccac gtcccgtgcg
      541 ccaggcgtcc acgctgtatc tttgtgcggc cggcagccag atcatcagaa aggtcaccag
      601 caattcgagg tagtggtgac tcatccagta ggttctcgtc gattccacga cgtataagta
      661 accccacgag aggaacagcg ccgcagccga ggccctatac aagagcccca gcgcgacggt
      721 aatgccggcg gcgacttgaa gacccaggat cagatgaatc caacgggccg gcaacatggg
      781 catccactcg aagcccgcgt acggaaacgt gaacttcacg ttcggcccag tgtaaaaggt
      841 ttccagaggg cttttcccca ttgtgctcgg cgaagcagtg cagagcgcgt atgcctccag
      901 caccattacc aggccgaccg cgatgcgaaa cacggccagc gaagcgccgc tgactggttt
      961 ctcgagcaac gattcgaaac gcgcccagac gccgcccgat gccgcgaccg ataccgatgc
     1021 acccgcgtgc tcaatcaagc cttgctgacc cgcgttcaga cccgcagcgc gctgcattct
     1081 ccgcagtttt cgtctgtctt tcatctggtc tatcgtttca tgttcatgcc ttcgtcgctc
     1141 gtccgaaaca gtctttcgac tttcggggca tcacgcttgc ggagtctcac tcgatcgcgc
     1201 attcagaccg tggagctaat tgctggtcac acgacaatgc cagcaacacg aagacttcca
     1261 aggcgggaat ttgattcttc gaagggagcg gtgactgttc ccaaatgtcc atgcgctcgg
     1321 attgcgccga atgacgaaaa agatcagttc gcctgagaaa cggtcggata aaaaaaggcg
     1381 ggctctggca taccgttcat tgccagagcc cgccttcctt ctcgggtcga aggaaatgtt
     1441 ggcgctcgac gcgaaggccg gatggagccc ggccttcgcg cgctttgcgt catcttaagc
     1501 ggaagaacct gttgccgctg gtcgcagcgg taaccgtgta ggtattgccg accggagtcg
     1561 gagtcacgtc agaccagtcg gttgctggat tgccggtgaa agcggtcgct tgttgcaaag
     1621 tgcccgtgcc ggtccaggtg atgatgatgt cggcgcccga gcgcgtcggc gtattgaacc
     1681 tcggccccgc gacagacgtc ctcgtgcgaa acgccttcag tccgccgccg gccacgtcat
     1741 tgaccaacac tttcgtcccg tcggctttga ccgagaacca ctcgcagttg gctccgccgc
     1801 cgccctgatg gaagatcatg cgcatgggat acacgcccgc ctgaggcacg atgaaactca
     1861 tgatcgtgtc agtggcgccg cgtccagtgt cgaagccgcc cacagtgaag tgagaatctc
     1921 cctgaatggt tcctgttacg gcaccgctcg caagcgcggc tttgagcttg gctgcatgga
     1981 catccgcgat ggacaaaacc ggaatggtaa ggtccgttct ggtggctcca gcgtagaatg
     2041 gcgtacccaa gtccgtaggc gtcgtgaaga ttactgcaac ggcgcccctg tcctgtgccg
     2101 ccttgcattt tccaccggtg ctggtc
//
LOCUS       DATMZQ010000304         4288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3357197, whole genome shotgun sequence.
ACCESSION   DATMZQ010000304 DATMZQ010000000
VERSION     DATMZQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3357197"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2513)
                     /locus_tag="VN496_09865"
     CDS             complement(108..2513)
                     /locus_tag="VN496_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF17300.1"
                     /translation="MRYPSRFSGSGRLQIPQWIALGLGTYVLVGGLVSFMGWAIDIPI
                     LADWDNDGIATEPNTAVVTMLTGAALILLNLAYRRSASVLGTMAALIALATLFEYVTG
                     ADLHIDSLLMFDRHWGQLGTLSSGRMGPPDCFAWTLGGAALMLCSCGRNARKFISLFG
                     LFMLGIAALSLTGYLFGVAVLYAWPRVTTIAFQTGTFIVAAGLSLIISVPEREPMRTL
                     LDDSTAGIMVRRTTPFVIALPIVLGYFQLQGQHAGYYDSALGTTVLVLALITLMCVVL
                     AQGVRAVRSWERVLKLANEAINRAEKKLNESRALIEAVNSGTPDLIFAKDREGRVTYL
                     NEATRVVVGKSLSQIIGRTEIDFIGDVPFSRTVMANDQRVMERAKAETVEETFVDGRT
                     FLITKSPLLDSGGNVVGVTGVGVDVTERKRMEQQLREADRRKDEFLATLAHELRNPLA
                     PITNALSILDRAGSDAWLNEQARSTMRRQIGQMVRLIDDLLDISRITLDKLELRKSAV
                     ELAPIVHHAVEACRPLIERAGHELSIDMPNEPVYLTGDSARLAQVFGNLLTNACKYTE
                     RGGRIGIIVTPRGTDVAVSIKDNGMGIPQNMLPQVFDMFVQIGRTIERTEGGLGIGLS
                     LAKRLVEMHGGTLTVQSDGPGRGSQFTVCLPMLPEGFKQPALDTKPPESANAAHRILV
                     VDDNRDSAESLAILLRLAGNDLQLAYDGVEAVEKAAVQNPDIIVLDIGLPKMNGYDAC
                     RAIRAQPGGKDITIIAASGWAREEDRRMSREAGFDAHLIKPVDHAILMKLVADAAARA
                     PNR"
     gene            complement(2656..3867)
                     /locus_tag="VN496_09870"
     CDS             complement(2656..3867)
                     /locus_tag="VN496_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17301.1"
                     /translation="MIHPLASSTRIPGSIWALGCVSLLMDVSSEMIHSLLPVFMFTVL
                     GISASAIGFIEGAAEATALLVKIFSGTLSDYWGKRKPLAVLGYGLGAFSKPLFALATG
                     IGLIVAARLLDRVGKGIRGAPRDALVADIAPTALRGAAFGLRQALDTVGAFLGPLLAM
                     GLMLLWTNDFRSVFWIAVIPAVLSVSLLLFGVQEPERRSDGAQLNPIRRENLRRLPRS
                     YWWIVAIGAAFTLARFSEAFLILRAQQGGLPLAFTPLVLIGMNLMYAISAYPFGKLSD
                     STDRATLLAWGVAVLITADVMLAWSSHWSWVGGGITLWGLHMGMTQGLLATMVADTAP
                     ADLRGTAYGFFNLVSGLALLIASTVAGLLWDRFGASFTFAAGAIFSALALAAILMHNR
                     VRANVAVLRND"
BASE COUNT          959 a         1304 c         1200 g          825 t
ORIGIN      
        1 cgaggaaacc ttgtccgtat tgcttgagac ggcccgctca gttggtgtcg aggcgcgcat
       61 cgacggagtg ccagcgctac ccatcgccaa ctaaagcgct ggcaagatta cctgttcggc
      121 gcccgggctg ctgcgtcggc aaccagcttc atgaggatgg catggtccac cggcttgatc
      181 agatgtgcgt cgaagccggc ttccctggac atgcggcgat cttcttcgcg tgcccaaccg
      241 ctcgctgcaa tgatagtgat gtccttgccg ccgggctgtg cccggatagc acggcaggcg
      301 tcgtagccat tcatcttcgg cagaccgatg tccaggacaa tgatgtcagg attttggacc
      361 gccgctttct caacggcttc gaccccgtcg tatgccaact gcaagtcgtt tccggccagc
      421 ctcaacaaga tggcgagcga ttctgcggaa tcgcggttgt catcgacaac cagaatgcga
      481 tgtgccgcgt tggcactttc gggtggtttc gtatccaacg caggttgctt gaaaccttcg
      541 ggtaacatcg gcagacatac cgtgaactgg ctgcctcgtc ccggcccatc gctttgaacc
      601 gtcaatgtgc cgccgtgcat ttcgaccaat cgtttggcca atgacagacc gatgcccaga
      661 ccaccttcgg ttcgttcaat ggtccggccg atctgaacga acatatcaaa cacctgcggc
      721 agcatgttct gcggtatgcc catgccgttg tccttgatgc tgaccgctac atcggtgccg
      781 cgcggcgtga cgattatgcc gatgcggcct ccgcgctcgg tgtacttgca ggcgttggta
      841 agcaggttgc caaacacctg ggcgagccgt gccgaatcgc ccgtcaggta aaccggctca
      901 ttcggcatgt cgatgctgag ctcatggccc gcgcgctcaa tcagtggacg gcatgcttca
      961 acggcatgat gaacgatcgg ggccagctcg acggcggatt tgcgtaattc caatttgtcg
     1021 agcgtgatgc ggctgatgtc caacaaatcg tcgatcagcc gaaccatttg tccaatctgc
     1081 cgccgcatcg tgctgcgcgc ctgttcgttg agccaggcat cgcttccagc gcgatccaga
     1141 atactcagcg cgttcgtgat cggagcgagc gggttgcgca gctcatgggc gagcgtggcg
     1201 agaaattcat ccttgcggcg gtcggcctcg cgcaactgct gctccatccg cttgcgctct
     1261 gtaacgtcca ccccaacccc ggtgactcca acgacattgc cgccgctatc gagcaatggc
     1321 gatttggtga tcagaaacgt tctcccgtcc acgaacgttt cttccaccgt ctccgccttg
     1381 gcgcgctcca tgacgcgctg gtcgtttgcc atgacggtgc gtgagaacgg gacatcgccg
     1441 atgaaatcaa tctctgtccg cccgataatt tgcgaaagcg actttccgac taccacgcga
     1501 gttgcctcat tcaaataggt gacccgaccc tcgcgatcct ttgcgaatat caggtctggc
     1561 gtgccggagt tcactgcctc gatgagcgcg cgggattcat tcaatttctt ctcggcgcgg
     1621 tttatagctt cgttggcgag cttgaggacc cgttcccacg acctgaccgc gcgcacacct
     1681 tgcgcaagta ccacgcacat cagtgtgatc aatgccagta ccagaaccgt tgtgcccagt
     1741 gcggaatcgt agtagcccgc atgctgtccc tggagctgga agtaacccag cacaatcggc
     1801 aacgcaatca cgaacggcgt ggtacggcgt accatgattc ccgcggtgct gtcgtccagc
     1861 agcgtacgca tcggttcacg ttccggtacg ctaatgatca gcgacagacc ggccgctaca
     1921 atgaacgtac cggtttggaa agcaatagtc gtgacccgcg gccatgcata gaggacggcc
     1981 actccaaaca gatatccggt taatgaaaga gcggctattc cgagcatgaa aagcccgaac
     2041 agtgaaatga atttgcgggc gttccggccg cacgaacaca acatcaacgc cgcgccgcca
     2101 agagtccagg caaagcagtc cggcggaccc attcgaccgg acgaaagtgt gcccaattgt
     2161 ccccagtggc ggtcgaacat caaaaggcta tcgatatgca gatctgcgcc agtgacgtat
     2221 tcgaataagg tcgccagcgc gatcaatgcg gccatcgtgc caagaacgga tgctgaacgg
     2281 cgatacgcca ggttgagcaa gatcagcgcc gcgccggtga gcatggttac tacggcggta
     2341 ttcggctccg tagcaatgcc gtcattgtcc cagtcagcca ggatagggat atcgatcgcc
     2401 caacccatga acgaaacgag tccgccaacc agcacgtacg tgccaagccc aagagcgatc
     2461 cactgcggaa tttgaaggcg gcctgatccg gaaaaacgcg atgggtaacg catgcgccag
     2521 gctcctccca agcagtcgag tcaatccgtt atcgtagtcc taaaagcaat ttttcgcgca
     2581 agcgagtccc atgactgagt atcgtttaca cggagaacgg aaaggtcgac aatcccggca
     2641 attctggcgt tgggttcagt cgtttcgcaa tacggctaca tttgcgcgca cgcggttgtg
     2701 catcaggatt gcggccaggg ccagggcact gaagattgcg ccggccgcga acgtgaaaga
     2761 cgcgccgaac cggtcccaca acaacccggc caccgtgctc gcaatcagca gcgccaatcc
     2821 cgacacaaga ttgaaaaagc catacgctgt gccgcgcaga tccgcaggcg ccgtgtcagc
     2881 taccatcgtg gcgagcaagc cttgcgtcat gcccatatgc aagccccaca gcgtgatgcc
     2941 tccccccacc caactccagt ggctgctcca ggccagcatg acgtccgccg ttatcaatac
     3001 ggcgactccc cacgccagca aagtggcgcg gtcagtcgaa tcggataact tgccaaacgg
     3061 atacgccgag attgcgtaca ttagattcat gccgatcagc accagcggag tgaacgccaa
     3121 tggcagtcct ccttgctgcg cgcgaagaat caagaatgcc tcggaaaaac gcgccagcgt
     3181 aaacgcggca ccgatcgcca cgatccacca atacgagcgc ggcagccggc gcaaattttc
     3241 acggcggatt ggattcagct gcgcgccgtc cgatctgcgt tccggctcct gcactccgaa
     3301 taacagtagg gacaccgaga gcacggccgg aatcacggca atccagaaca ccgaacggaa
     3361 atcgttcgtc cacagcaaca tcaatcccat cgccagcagc ggtccaagga atgcgccgac
     3421 cgtatcgagc gcctggcgca atccaaaagc agcgccgcgc agtgccgtcg gcgcaatgtc
     3481 tgcgaccagc gcgtcgcgtg gcgcaccgcg aatgcctttg ccgacgcggt caagcaggcg
     3541 ggcggcaacg atgaggccga tgccggttgc cagtgcgaac agcggtttgg aaaacgctcc
     3601 aagaccgtag ccgagtacgg ccagcggctt gcgcttgccc cagtaatccg acaaggtgcc
     3661 agagaaaatt ttaacgagaa gagccgtggc ctcggcggct ccctcaatga acccaatcgc
     3721 ggaggcgctg atacccagca ccgtaaacat gaacaccggg agcaggctgt gaatcatttc
     3781 cgatgagacg tccatcagca ggctcacaca gccgagcgcc caaatacttc ccggtatgcg
     3841 cgtggaagat gccagcgggt gaatcatttc agcatggcgt ggattgaacc ctcaaaaatg
     3901 gcgcactcaa ttgagcggca agacaaccca acgccgccat tatcagtcaa tcagtagtgt
     3961 gatgtattgg cacgttcaat cagcctttga gcagcaaagt catcgctgcc aaaggacggc
     4021 attaatacgc ggcgtctgac ggcctcagct gttctttatg aattgtcgcg ctcttaccct
     4081 acgcggcctg cgactcgagt agagccgcgc tttagtgtgc cgcggcggcg tcggtgcggt
     4141 aaatggcaaa cgccttgttc agcacgtctc gcgtcaaaaa acctgccgaa tcgaatacgt
     4201 gtgaatgaac cggattacga taggcaacga cgagcgaacg aggtctggag cggtaagcgt
     4261 tctccatatt ccggcatacc tcggataa
//
LOCUS       DATMZQ010000305         5772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_818404, whole genome shotgun sequence.
ACCESSION   DATMZQ010000305 DATMZQ010000000
VERSION     DATMZQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818404"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VN496_09875"
     CDS             <1..758
                     /locus_tag="VN496_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HXF17302.1"
                     /translation="QSLGLGKQAPEIVVAFLKLGPMVMTSIAFGFLYRTVPNRQVTVL
                     DAAVGGIIASFAFEAMKIVFGHFIANFASYKLVYGTFASLPILLVWIYLSWVVIVFAS
                     VITAVLPYWRSGGVLLKQAPGSQFVAAMEMLKMLYRAYLDGYVLNLQQLRTALKLSWE
                     EAEAILEKLVNAGWVAKLQGNGWVIARDASNITVKDIFRLFVFGRDAAEVSEESAIRK
                     LVASVSSTVDGELSFSLKELFATGSSTRAARVA"
     gene            767..1228
                     /locus_tag="VN496_09880"
     CDS             767..1228
                     /locus_tag="VN496_09880"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXF17303.1"
                     /translation="MRNRYRLLALLLLCTAAIAAADDFTVTDTQGKTHKLSAYRGQWV
                     IVNFWATWCPPCLEEIPDLVAIKESHKDLQVIGIAMEFQDARQVTQFAEGMFVNYPIV
                     LGDRTISQNIGRVDGLPTTFIYDPKGKLADRHVGKITRKQIEQLISGKNAG"
     gene            complement(1235..1882)
                     /locus_tag="VN496_09885"
     CDS             complement(1235..1882)
                     /locus_tag="VN496_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXF17304.1"
                     /translation="MRNAMHGAVLFICALLSQAGVAGEQQYEALSANVRAMLHHSVAD
                     RASPRLTFESQTDGAVWLAEMSKRLENHIPEATTRHDFLVTLQYEASRAGLDPQLVLS
                     VIQVESKFRKYAVSKAGARGYMQVMPFWVKLIGDSQQNLFNLRTNLRYGCTILRHYLT
                     IEHGDMVRTLARYNGSLGQSRYSNNVLRAWSGNWDYARGKPTIIIAPTRYVGEGG"
     gene            complement(1897..3606)
                     /locus_tag="VN496_09890"
     CDS             complement(1897..3606)
                     /locus_tag="VN496_09890"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXF17305.1"
                     /translation="MRTSQFFISTLKEAPSEAELISHKLMLRAGLIRKLGSGLYTWMP
                     LGLRVLRKVEAIVREEMNRAGGVELLMPAVQPAELWQETGRWDLFGPQMLKIKDRHER
                     DFCFGPTHEEVITDIARREIRSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFLMKDA
                     YSFHASYEDLEREYRNMYETYSRIFTRLGLRFRAVAADTGAIGGTGSHEFHVLADSGE
                     DAIAYATDSDFAANIELAEAVPSLKHRAAPGKTMQKVATPGKTTCEEVTALLGVPLSQ
                     SVKALAVMAEERMSMLLVRGDHQLNEVKASKLPGLTTFRFATDKEIEGTIGCRPGYIG
                     PVGVPAAVRVITDRSVAAMSDFVCGANEPGFHLTGVNFGRDLPEPTMVADIRNVVAGD
                     PAPDGRGALAICRGIEVGHIFQLRTKYAEAMKAMFLDEEGKSKALEMGCYGIGISRIV
                     AAAIEQGNDEKGIVWPLPMAPFQLAIAPIGYRKNAQVKEVADRLHDALQAAGIDVLLD
                     DRDERPGVMFADLELIGVPHRVTIGERGLKESKLEYQGRNDKSAAAIDLQGASDHLVS
                     LCR"
     gene            3678..4457
                     /locus_tag="VN496_09895"
     CDS             3678..4457
                     /locus_tag="VN496_09895"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HXF17306.1"
                     /translation="MLGKWRITRSVNRDVNVEVSEKDGIRSLHLGSDTVQSSMKLDDP
                     YELVLSYTRAMMAFLLFRPHPEHVVMVGLGGASMPKFVHRHLQSTRTTVIEIEPKVIA
                     IASQYFHLPPQDARLQVVTGEGGAWVASHPGCCDVLMVDGYDSREQVAQLCSEDFYAN
                     TRAALSANGVLVVNLWSSDSRYDVYLQRMESVFEAVVCVPAERRGNVAVLAFRKSPGQ
                     PRWDELRIAARTLQSLYGLEFLNFVEGIRELNPRSAARLLV"
     gene            4533..5021
                     /locus_tag="VN496_09900"
                     /pseudo
     CDS             4533..5021
                     /locus_tag="VN496_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            complement(5041..5490)
                     /locus_tag="VN496_09905"
     CDS             complement(5041..5490)
                     /locus_tag="VN496_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HXF17307.1"
                     /translation="MTKRFREVIESRVHGVFRCHAVIPDTALEFAVQHDLTAIPIRLA
                     ATRDKKGFLNAVAQALQFPDYFGHNWDAFYDCLVELKLGREGTLLLLHDASNFARAEP
                     EEFAAAAAALQDATDYWKEKGQLLTVIVELKTPALAPELQEIIPPGA"
     gene            complement(5487..5768)
                     /locus_tag="VN496_09910"
     CDS             complement(5487..5768)
                     /locus_tag="VN496_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HXF17308.1"
                     /translation="MEVAVVRAAELSEQARETLALIKRGGPYPYEQDGAVFANRERNL
                     PIAPRGTYREYTVKTPGRRDRGGRRIVAASTGEFWYSDDHYRTLRRIVE"
BASE COUNT         1194 a         1626 c         1646 g         1306 t
ORIGIN      
        1 cccagtcgct gggcctgggc aagcaggcac ccgaaatcgt cgtcgcgttc ctcaagctcg
       61 gaccaatggt gatgacatcg atagcatttg gatttttgta ccggaccgta ccgaaccggc
      121 aggtcacggt ccttgatgcg gccgtcggcg gcatcattgc ttcctttgca ttcgaggcca
      181 tgaaaatcgt cttcgggcat ttcatcgcta atttcgccag ctacaagctc gtctacggca
      241 cgttcgccag cctgccgatt ctgctggtat ggatctatct gtcatgggtg gtgattgtgt
      301 ttgcctcggt catcaccgca gtgctgccgt actggcgcag cggaggcgtg cttctgaagc
      361 aagcgccggg ctcgcaattt gttgcggcca tggaaatgct caaaatgctc taccgggcgt
      421 atctggacgg ctacgtactc aatttgcaac aactgcgcac ggcactgaaa ttgtcatggg
      481 aagaagcaga agccatcctt gaaaagttgg tcaatgccgg ctgggtcgcc aaactgcagg
      541 gcaacggttg ggtcattgcg cgtgatgcat ccaatattac ggtcaaggat atcttccgtc
      601 tgttcgtttt cggtcgggac gctgcggaag tcagcgagga atccgctatc cgcaaactgg
      661 tcgcgagcgt atcttcgacg gtcgacggcg aactgagttt cagtctgaag gagttgtttg
      721 caacgggaag cagcacgcgc gctgctcgcg tagcctgaac ggcatcatgc gaaaccgcta
      781 tcgtttattg gcgttgctgc tgctttgtac agcggccatt gcggcagctg atgattttac
      841 ggttacagat acgcagggca aaactcacaa gctttccgct tatcggggcc aatgggtgat
      901 agtgaatttc tgggcaacat ggtgtccgcc gtgcctcgag gaaattcccg atctggtcgc
      961 aattaaagaa tcgcacaagg acctgcaagt cattggcatc gcgatggagt ttcaggatgc
     1021 caggcaggtc acccaatttg ccgaggggat gttcgtgaac tatccgattg tgctcggcga
     1081 tcgtacgatc tcgcaaaaca ttgggcgagt cgacggcctg ccgaccacgt tcatctacga
     1141 tccgaaaggc aagctggccg accgccatgt cggcaagatt acgcgcaagc agatcgagca
     1201 gcttatctcc ggcaagaacg ccggctgaac gcaatcagcc gccttcaccg acatagcgcg
     1261 tcggcgcaat gattatcgtc ggcttgccgc gcgcataatc ccagtttcca gaccaggcgc
     1321 gcagcacgtt gttggagtag cgcgactggc cgagcgagcc gttgtagcgc gcgagcgtgc
     1381 gcaccatgtc gccatgttcg atggtcaggt aatggcgcag gatagtgcag ccatagcgca
     1441 gattggtacg cagattgaag agattctgct gactatcccc gatcagtttc acccagaatg
     1501 gcatcacctg catgtatccg cgtgcgccgg ctttcgacac ggcatatttg cgaaatttgc
     1561 tttccacttg aattacgctg agcacgagtt gcggatcgag tccagcccgc gaagcctcat
     1621 actgcagggt aaccagaaaa tcgtggcggg tggttgcctc ggggatgtgg ttttctagcc
     1681 gctttgacat ttccgcgagc catacggctc catcggtttg cgactcaaag gtcaggcgcg
     1741 ggctggcacg atcagccacc gaatgatgca gcattgcccg cacgtttgcg gacagcgctt
     1801 catattgctg ttcgcccgca acgccggctt gtgacagcaa cgcgcatatg aacaaaactg
     1861 cgccgtgcat ggcgttacgc atgagtttgt cttttctcac ctgcaaagcg agacgagatg
     1921 atcggaagca ccctgcaagt cgatcgctgc tgctgattta tcgttgcgtc cctggtattc
     1981 gagcttgctc tccttcaggc cccgctcgcc tatggttacc ctatgcggaa cgccaattaa
     2041 ttccaggtcc gcgaacatta cgcccgggcg ttcatcacga tcatcgagca gcacgtcgat
     2101 gccagctgcc tgcaacgcgt cgtgcaaccg gtcggcgact tctttgactt gcgcattctt
     2161 gcgatagccg atcggcgcga tggccagctg gaacggcgcc attggcaacg gccagacgat
     2221 gcccttttcg tcgttcccct gctcgatggc cgcggctacg atgcgtgata tcccgatgcc
     2281 atagcacccc atttccagcg ctttggattt gccctcttcg tccaggaaca ttgctttcat
     2341 tgcttcggca tatttggtgc gcagctggaa gatgtgtccc acttcgatgc cgcggcatat
     2401 tgccagggcg ccccggccgt ccggtgctgg atcgcccgcg accacattgc gaatgtctgc
     2461 caccattgtg ggttcgggca gatcacgacc gaagtttacg cctgtcaggt gaaatcccgg
     2521 ttcattggcg ccgcatacaa aatcgctcat ggcggccacc gaacgatcgg tgatcacccg
     2581 caccgcggcg ggtacaccca ccggtccgat gtagccgggc cggcaaccaa ttgtcccttc
     2641 aatctccttg tcggtggcaa agcgaaaagt agtgagtcca ggcagcttgg atgctttcac
     2701 ctcgttcagc tggtgatcgc cacgcaccag caacatgctc atgcgctcct cagccatcac
     2761 ggcaagcgcc ttgaccgact gtgacagcgg tacgcccaat aacgcggtca cctcttcgca
     2821 agtagtcttg ccgggcgtag ccactttctg catggtcttg cccggtgcgg cacgatgctt
     2881 tagggatggc acggcctcgg cgagttcgat attggcggcg aaatccgagt cggtcgcata
     2941 agcaatggca tcttccccgg aatccgccag cacatgaaat tcgtgcgacc cggtcccgcc
     3001 gattgcacct gtatccgcag caacagcgcg gaatcttagg ccaagccgcg taaaaatcct
     3061 gctataggtc tcgtacatat ttcgatattc gcgttccagg tcttcataac tggcatgaaa
     3121 ggaatacgcg tctttcatca ggaattcacg cgcgcgcatg acaccgaaac gcggcctgat
     3181 ctcgtcacgg aacttggtct ggatctggta aaaatttagc ggcaattggc gataagagcg
     3241 gatctccctg cgcgcgatat cggtgatgac ttcttcatgg gtcggcccaa aacagaaatc
     3301 gcgctcatgg cgatccttga tcttcagcat ttgtggacca aataggtccc agcgcccggt
     3361 ttcctgccac agttcagcgg gctgcacggc cggcatcagc aactccaccc ctccggcacg
     3421 attcatttct tcgcgcacga tggcctccac ctttcggagc acccgcaagc cgagcggcat
     3481 ccaggtatat agaccactgc cgagtttcct gatgaggccg gcgcgcaaca tcagcttatg
     3541 gctgatcagt tccgcttcgc tgggcgcttc cttgagcgtt gaaataaaga actgcgatgt
     3601 gcgcatgctt ctgattgcgt tatcgaaaag cggctattct acaggccaac gcgtggcaac
     3661 ttggggaaga gggcaacttg ctcggaaagt ggcgaatcac tcgcagcgtc aatcgcgatg
     3721 tcaacgtgga ggtcagcgag aaagacggca ttcgctccct gcacctgggc agcgacacgg
     3781 tgcaaagctc aatgaagctc gacgatccct atgagctcgt gttgtcctat acgcgggcga
     3841 tgatggcttt tctgctgttc cggccgcacc cggaacacgt tgttatggtt ggcctcggcg
     3901 gggcctcgat gccaaagttt gtacaccggc acctgcagag tacccgaacc acagtcattg
     3961 agatcgaacc caaagtaatt gccattgcga gtcaatattt tcatttgccc cctcaagatg
     4021 cgcgtctgca agtagttacg ggggaaggcg gcgcctgggt cgcttctcat ccgggttgct
     4081 gcgacgtact catggttgac ggctacgaca gccgggaaca ggtcgcccaa ctgtgttccg
     4141 aggattttta tgcgaatacg cgcgctgcgc tgtcggcgaa cggcgtcctg gtcgtgaacc
     4201 tatggagttc ggacagccgc tacgacgtgt atttgcagcg catggaaagc gtatttgagg
     4261 cagtggtgtg tgtgccggca gaaaggcgag gcaacgttgc agttcttgcc tttcgcaagt
     4321 cgcccggaca gccgcgctgg gacgaattgc gtatcgcggc ccgcactctt caatcgctat
     4381 acggccttga atttcttaat tttgtcgaag gtattcgcga gctaaatccg cgctcggcgg
     4441 cgcgcctgct ggtctgatgc cggattgcga aactttgttt atgacgacat ttgtggaaaa
     4501 atccgttcat tgaatcgttt tgggtggctc acatgattga tcgtgatggc tatcgcccga
     4561 atgtaggaat cattctttgt aatggcagaa acgaggtttt ctggggtaag cggataaagg
     4621 aacatgcttg gcagttcccg caaggcggga tcaagggcgg tgagacgcct gagcaagcga
     4681 tgtaccgcga actcaaggag gagatcggcc tcgatccgtg ccatgtgaag atcctcgggc
     4741 gcacacgtga atggttgcgc tacgacgtac cacagacttg ggtgcgacgt gaaacgcgca
     4801 cacagtatcg ggggcagaag cagatctggt ttttgctccg gctggtgggc agggactgcg
     4861 acgtctgtct gcgccaatcg gagcatcccg agttcgatgc atggcgctgg catgaatact
     4921 ggattcccct ggaaaacgtc atcgaattta agcgcgatgt ttatcaacgt gcgttgcagg
     4981 aactggtgcg gtatctcaat ggttctcgtg tgaaacgaga cgctggcgca cgcggaccac
     5041 ttacgcccct ggagggatga tttcctgaag ctcgggtgcc agagcagggg tcttgagttc
     5101 cacaataaca gtcagtagct ggcctttctc tttccaataa tcagtcgcat cctgcagtgc
     5161 ggctgccgcg gccgcgaatt cttccggttc cgccctggcg aaatttgaag cgtcgtgcag
     5221 caacagtaac gtgccctcac ggccaagttt gagctcgacc agacagtcgt aaaaagcatc
     5281 ccagttatgg ccaaaatagt cgggaaattg aagcgcctgg gccactgcgt tcagaaaacc
     5341 tttcttgtcc cgggttgctg caaggcggat gggaatcgca gtcaagtcat gttgcaccgc
     5401 aaactccagc gccgtgtctg gtatgacggc atgacatctg aataccccat gcacgcgcga
     5461 ttcgatgact tcccgaaatc ttttggtcat tcaacgatcc gcctgagagt gcggtaatgg
     5521 tcgtcgctgt accagaattc accggtactg gccgcaacga tccgtctgcc gccgcgatca
     5581 cgtctgcccg gtgtcttcac cgtgtattcc cggtaggtgc cacgcggtgc aatcggcagg
     5641 ttgcgctcgc gatttgcgaa cacggcaccg tcctgctcat agggatacgg gccgcctcgc
     5701 ttgatcagcg ccaaagtctc gcgcgcctgc tcggacaatt cagccgcgcg cacgacggcg
     5761 acttccaccg cg
//
LOCUS       DATMZQ010000306         3737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2666832, whole genome shotgun sequence.
ACCESSION   DATMZQ010000306 DATMZQ010000000
VERSION     DATMZQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666832"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VN496_09915"
     CDS             <1..1244
                     /locus_tag="VN496_09915"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXF17309.1"
                     /translation="EFIELVASAALSPGHCNTMGTASTMNSLAEALGMSLPGSAAIPA
                     PHKDRAVNAYESGKRIVDMVWEDLRPSKIMTREAFENVIVVNSAIGGSTNAPVHFNAI
                     AKHIGVALDNDDWEKIGYDIPLLVNLQPAGEYLGEEYHRAGGVPAVVSELIKAGKIRK
                     NPITVNGKTLYENCRKAVVDDQKVIWPYDRPMKKSAGFLNMKGNLFDSAIMKTSVISE
                     AFRKRYLSNPKDPNAWEGRAIVFDGPEDYHHRIDDPKLKIDDDCMLFVRGTGPKGYPG
                     AAEVVNMQPPARLIKEGITELPCIGDGRQSGTSGSPSILNASPEAASMGGLAILKTGD
                     QVRIDLNKRTANILISKTEYAQRMKALEAHGGFKSPASQTPWQEMQRDMVDELSNGMV
                     LKPAVKYQRISTSKGTPRDNH"
     gene            1372..1782
                     /locus_tag="VN496_09920"
     CDS             1372..1782
                     /locus_tag="VN496_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17310.1"
                     /translation="MRTVPIAAILACLSMTCLADDDSDIVTGCLMSNAEFGTEMAQIC
                     IRDNQAARAEVARYPDEYKETVQRCTRRKDMGWGIVKKCIDDDIAAGPALEAYSRDHA
                     ALLEHCQRDFPGREATRIKLCVDQALEAKTNEAK"
     gene            complement(1798..2556)
                     /locus_tag="VN496_09925"
     CDS             complement(1798..2556)
                     /locus_tag="VN496_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17311.1"
                     /translation="MSKIAMITGATSGFGAAAVRRFAKAGWRVVATGRRIERLKQLAV
                     ECGGDKVFVSSFDMRKPEAIEAALDNLPDSFRDIDLLINNAGLALGTAPAQTANLDQW
                     NQMIETNVTGLVTITHKLLATLIARKGAIINISSVAASYPYPGGNVYGGTKAFVTQFS
                     LGLRSDLHGTGVRVTSVEPGMAETEFTLVRTGGNQEASDKLYKGADPMTAEDIAETLF
                     WIATLPPHMNINRIEVMPVRQSFTGFQVDRVPAG"
     gene            complement(2553..>3737)
                     /gene="glcF"
                     /locus_tag="VN496_09930"
     CDS             complement(2553..>3737)
                     /gene="glcF"
                     /locus_tag="VN496_09930"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HXF17312.1"
                     /translation="DSILRTCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKEMLEGN
                     EVTAKTQIHLDRCLTCRACETTCPSGVEYGRLVDIGRNIVEQHVPRSPLDALKRATLR
                     VVLPHPALFGPLLRLGQTLRAILPAALKKKIPERQTHGTHWPAPRHARKMLVLDGCVQ
                     PSLAPDINPATARVLDRLGIDLVKADTAGCCGAVTFHLNRQEDARDFMRRNIDAWWPH
                     VEAGVEAIVVTASGCGTLVKEYGHHLAQDPRYAAKAAKISVLSKDVSEVVFAERAALE
                     QLLALAPAPSMRPRVAFHTPCSLQHGLKIHGMVEAILLAAGFELTLVPDAHLCCGSAG
                     TYSILEPELSKRLLQNKVVALESGSPSLIATANIGCLTHLQSASALRVKHWINLLDER
                     LS"
BASE COUNT          795 a         1079 c         1127 g          736 t
ORIGIN      
        1 aggagttcat cgagctggtc gcctcggcgg cgctgtcgcc gggtcactgc aacacgatgg
       61 ggaccgcgtc gacgatgaac tcgctggccg aagcgctcgg catgtccctg cccggctcgg
      121 ccgccatccc ggcgccgcac aaggaccggg cggtcaatgc gtacgagagc ggcaagcgca
      181 tcgtggacat ggtgtgggag gacctgcggc cgtcgaagat catgacgcgc gaggcgttcg
      241 agaacgtgat cgtcgtcaac tcggcgatcg gcggctcgac gaacgcgccg gtccacttca
      301 acgcgatcgc gaagcacatc ggcgtcgcgc tcgacaacga cgactgggaa aaaatcggtt
      361 acgacattcc gttattggtc aacctgcaac cggccggcga gtatcttggc gaggaatatc
      421 atcgcgccgg cggtgtgccg gccgtggtga gcgaactcat caaagcaggc aagatccgca
      481 agaaccccat taccgtcaat ggcaaaacgc tgtacgagaa ttgcaggaag gctgtcgtgg
      541 acgatcagaa ggtgatctgg ccatacgatc ggccaatgaa gaaatcagca ggcttcctga
      601 atatgaaagg caacctgttc gatagcgcca tcatgaagac cagcgtcatt tctgaggcgt
      661 ttcgcaagcg ctacctgtcg aatcccaagg atccgaacgc atgggaaggc cgggccattg
      721 tattcgatgg tccggaggac tatcaccatc gcattgacga tccgaaacta aagattgacg
      781 atgactgcat gctgttcgtg cgaggcaccg ggcccaaggg gtatccgggc gcagcggaag
      841 tggtcaacat gcaaccgcct gccaggctga tcaaggaggg catcaccgaa ctgccgtgca
      901 ttggcgatgg gcgccaatcg ggcacatcgg gctcgccgtc gatcctgaac gcttccccgg
      961 aagcggccag catgggtgga ctcgcgattt tgaaaaccgg cgaccaggtt cgcatcgatt
     1021 tgaataagcg tacggcgaac atcctgatca gcaagaccga gtatgcgcag cgcatgaaag
     1081 cactggaggc gcacggcggc ttcaaaagcc ccgccagcca gacgccgtgg caggagatgc
     1141 agcgcgacat ggtggatgaa ctgtccaacg gcatggtgct caagccggcc gtgaagtacc
     1201 agcgcatctc tacgagcaaa ggcacgcctc gggataatca ttgaggtcaa ttgattttcg
     1261 gtggtttgaa aatggagcag cgcactggtc tgtgctatgg ccgacaagac ccgttagttc
     1321 agactggaag ctgttcccgt tcaagcgtca atatttttgt gcggtcacat tatgagaacg
     1381 gttccaatcg cagcaatatt ggcttgtctg tcgatgacat gcctggcaga tgacgattcc
     1441 gacatcgtca ctggctgcct gatgtccaac gcggaatttg gcaccgaaat ggcgcagatc
     1501 tgcatcaggg acaatcaggc tgcgcgtgcc gaagtggccc gttacccgga tgagtacaag
     1561 gaaaccgtgc agcgttgcac ccgccgcaag gacatgggat gggggatcgt caaaaaatgc
     1621 atcgatgatg acattgccgc tggcccggcg ctggaagctt attccaggga tcacgcggcg
     1681 ctgctggagc attgtcagcg tgatttccca ggccgggagg cgaccagaat aaaattgtgt
     1741 gtcgaccagg cactggaagc aaaaaccaac gaggccaagt aatcgccctg cgggtgctta
     1801 gccggctgga acccgatcga cctgaaagcc tgtaaaagac tgacgcacag gcatgacttc
     1861 gattcgattg atgttcatgt gcggtgggag cgtggcgatc cagaacagcg tttccgcgat
     1921 atcctccgcg gtcatgggat cggcgccttt gtagagtttg tccgaggctt cctgattgcc
     1981 gccggtgcga accagcgtga actcggtctc cgccatgccc ggttcaaccg atgtcacacg
     2041 caccccggtt ccgtgcaaat cagaccgcag tcccagcgaa aattgcgtaa cgaaagcctt
     2101 ggttccgccg tagacattgc cgcccgggta gggatagctg gccgccaccg agctgatgtt
     2161 gatgatcgcc cccttgcgcg cgatcagcgt tgccagcagc ttgtgcgtaa tcgtgaccag
     2221 gccggtcaca ttggtctcga tcatttgatt ccactgatcg aggtttgctg tttgcgcggg
     2281 cgcggttccg agggccaggc cggcgttatt gatcagcaga tcgatatccc tgaatgaatc
     2341 cggcaaattg tcgagtgccg cttcgatggc ctccggcttt ctcatgtcga acgagctgac
     2401 gaagaccttg tcaccaccgc attcgactgc cagttgcttc agtcgctcga tccgacgccc
     2461 ggttgcgacc acacgccagc cagccttggc gaacctgcgc actgccgccg cgccgaaacc
     2521 cgaagtcgcg cccgtgatca ttgcaatctt gctcatgaca gcctttcgtc cagcaggttg
     2581 atccaatgct tcacgcgcag cgcgctcgcg ctttggagat gggtaagaca gccgatattt
     2641 gcggttgcga tcagcgaagg cgagccagac tccagcgcaa caaccttgtt ctggagcaac
     2701 cgttttgaca attccggttc cagtattgaa tacgtaccgg cggagccaca gcataaatgc
     2761 gcgtccggta ccagtgtaag ctcgaacccc gccgccaaca ggattgcttc caccatgccg
     2821 tgaatcttca gaccatgctg cagcgagcag ggcgtatgga atgcaacgcg tggcctcatt
     2881 gatggcgctg gagccaatgc caacaattgt tccagcgctg cccgttcggc aaaaaccacc
     2941 tcgctgacat cttttgacaa taccgagatc ttcgccgcct tggccgcgta gcgcggatcc
     3001 tgtgccaggt gatgaccata ttccttcacc agcgtgccgc agccgcttgc tgtgacgacg
     3061 attgcttcga cgccggcttc cacatgcggc caccaggcat cgatattcct gcgcatgaag
     3121 tcgcgtgcgt cctcctgtcg gttcaggtgg aatgtgacgg cgccacagca gccagccgta
     3181 tcggctttca caaggtcaat gccaaggcgg tccagtacgc gcgcggttgc cggattaatg
     3241 tcgggggcaa gcgaaggctg tacgcagccg tccagcacga gcatcttgcg ggcgtggcgc
     3301 ggcgcgggcc aatgcgtgcc atgggtctgc ctttccggga tctttttttt cagcgcggca
     3361 ggcagtatcg cgcgcagcgt ctggcctagt cgaagcaatg gtccgaaaag ggcagggtgc
     3421 ggcaaaacca ctcgcagcgt cgcgcgcttc agtgcatcga gcggcgaacg cggcacatgt
     3481 tgttcgacaa tattgcggcc gatatccacc agcctcccgt actcgacgcc ggacgggcag
     3541 gtcgtttcgc acgcgcggca ggtcaggcat ctatcaagat gaatctgtgt cttcgcggtc
     3601 acttcgttgc cttcgagcat ttccttcata aggtaaatgc gcccgcgcgg gccgtcgagt
     3661 tcatcgccga gcagttgata ggtcgggcag gtcgccgtgc agaagccgca gtggacacag
     3721 gtgcgcagga tgctgtc
//
LOCUS       DATMZQ010000307         3092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3017164, whole genome shotgun sequence.
ACCESSION   DATMZQ010000307 DATMZQ010000000
VERSION     DATMZQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017164"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VN496_09935"
     CDS             complement(<1..453)
                     /locus_tag="VN496_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXF17313.1"
                     /translation="MSFTVTVADLDQSVPAEEGQTVLDALLAHGVGFAYSCQAGNCGT
                     CRCELISGDIMELEYSEHALSAEERSRGLVLACRSQVWGDVVVRRLEAEDFVVHPSRV
                     LRCKVADLTPLTHDILRLRMEILSGGPFTFSAGQFASLEFPVAAGAVRD"
     gene            522..1004
                     /locus_tag="VN496_09940"
     CDS             522..1004
                     /locus_tag="VN496_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXF17314.1"
                     /translation="MDVKQSRARPTPRAARHRKAPALGVLPGLIGYQLRLAQRAIFAD
                     FADTVGESGISPGLFGILVVIGENPGLTQQALANAAHLDRSTVVTVIDKLEARGLVKR
                     HAADRRSNGLFLADEGTALLRKLKRKVATHERRVVRELSPGERVQLVSLLQRILPERR
                     "
     gene            1017..2099
                     /gene="corA"
                     /locus_tag="VN496_09945"
     CDS             1017..2099
                     /gene="corA"
                     /locus_tag="VN496_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HXF17315.1"
                     /translation="MARKTTHRKKLFRSRGKPGLAPGSLVYTGEKKTLVPRVTMLDYD
                     EHRVVEKQLQRVDDCIPFKDAQTVTWINVDSVTEPGLLENFGKVMGFNALMLEDILNT
                     DQRPKFEDYGDYIYIVAKMLDWDNARGEIEIEQLSLIIGQNYVITFQERAGDFFDPLR
                     ERIRRSLGRIRKLGTDYLAYAILDIIVDHYFVVLEKLGEKIEHLEEVIITRPAQDTVR
                     AVYAIKREMIFVRKSVWPLREVVTGLQRSESVLVRESNAAFFRDLHDHVMQVTDSVDT
                     FRDLLNGMLDSYYSTITTRTNSVMKVLTLFSAIFMPLTFITGIFGMNFRNFPELDWHY
                     GFQGSMILMVAVIIIMISIFKWKNWL"
     gene            complement(2133..2543)
                     /gene="arfB"
                     /locus_tag="VN496_09950"
     CDS             complement(2133..2543)
                     /gene="arfB"
                     /locus_tag="VN496_09950"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXF17316.1"
                     /translation="MRQFEVPEAELEFRHVRASGPGGQNVNKVSSAVELRFDIGASRS
                     LPADVKTRLRRLGGRRVSALDVLVIDADRFRTQQANRRDALVRLNELVHAAMIKPKKR
                     IATRPSRAAKERRIAAKRTRSIVKRARGRVSDAD"
     gene            2850..>3092
                     /locus_tag="VN496_09955"
     CDS             2850..>3092
                     /locus_tag="VN496_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17317.1"
                     /translation="MKRWTYTGVAKVLSSTAVLTALFSTGSLFGADVDGNRLLNADKE
                     LEKGNWLTYHGSYKSWHYSPLDQINTKTVGKLSEAWS"
BASE COUNT          690 a          900 c          855 g          647 t
ORIGIN      
        1 atcgcgcact gcaccggcgg caaccggaaa ttcgaggctg gcaaactgcc cggccgaaaa
       61 tgtgaatggg ccgcctgaga ggatctccat gcgcagccgc agaatatcgt gcgtaagcgg
      121 cgtcaggtcg gccactttgc accgcaatac gcgcgacgga tgcacgacga aatcctcagc
      181 ctcgagccgg cgcaccacca catcccccca aacctgcgag cggcaggcaa gcaccaggcc
      241 gcggctgcgc tcttcggcgg acaaagcatg ctccgaatac tcgagttcca tgatgtcgcc
      301 tgaaatgagt tcacaacggc aggtgccgca attgcccgcc tggcagctgt aggcaaaacc
      361 cacgccgtgc gccagcaagg catccaggac tgtttgtcct tcttcggccg gaacggattg
      421 atcaaggtcg gcgacggtaa cggtgaaact cacggaattg ctccgctggc agggaatggg
      481 ccgatgaact aatatcgtct acaagtatgg gaagcaaact gttggatgtc aaacagtccc
      541 gcgcgcggcc gacgccgcgc gcggcacgcc accgcaaggc gccggcactc ggtgtgttgc
      601 caggcctgat cggctatcag cttcgtctcg cacagcgcgc gatctttgcc gatttcgccg
      661 atacggtcgg agaaagcggc atttcacccg ggttgttcgg cattctggtc gtgattggcg
      721 aaaatccggg ccttacccag caggcgctgg ctaatgccgc gcatctggat cgctctactg
      781 tcgttacggt gatcgacaag ctggaagcac gcggcctggt caagcgccat gctgcggacc
      841 ggcgaagcaa cggcctgttt ctcgcggacg agggcacggc gctgctgcga aagctgaagc
      901 gcaaagtcgc gacacatgag cgccgcgtgg tgcgggagct atccccgggc gaacgtgtcc
      961 aactggtctc gctgctacag cgtatcctgc cggaacgacg ctaacgaaag acgacggtgg
     1021 cccgcaaaac gacccatcgc aagaagctct tccgctcccg cggtaaaccc gggctggctc
     1081 ccggttcgct ggtttacacc ggcgaaaaaa agacgcttgt tcctcgcgtc accatgctgg
     1141 attacgacga acaccgagta gtcgagaaac agctgcagcg ggtagatgac tgcattccgt
     1201 tcaaggatgc gcagactgtt acctggatca acgtcgacag cgtcaccgaa cccgggttgc
     1261 tggaaaattt cggcaaggtc atgggcttca atgcgctgat gctcgaggat atcctcaata
     1321 ccgaccagcg tcccaagttc gaggattacg gcgactacat ttatatcgtc gcaaaaatgc
     1381 tggattggga caacgcccgt ggcgaaattg aaatcgagca attgtccttg atcatcggac
     1441 agaactatgt gattacattc caggagcgcg caggagactt tttcgacccg ttgcgcgaac
     1501 gcattcgccg gtcgctggga cgcatccgca aactgggcac cgactatctc gcctatgcca
     1561 tcctcgacat catcgtcgat cactattttg tcgtgctcga aaaactcggc gaaaaaatag
     1621 aacacctgga agaagtcatc atcacccgtc cggcacagga taccgtgcgc gcggtgtatg
     1681 ccatcaagcg cgaaatgatt ttcgtgcgca aatcagtctg gccgctgcgc gaagtggtca
     1741 cgggactgca acgttcggaa tcggtcctgg tgcgcgaatc aaatgcggcg ttttttcgcg
     1801 acctgcacga ccatgtcatg caagtcaccg acagcgtgga taccttccgc gatctgttga
     1861 acggcatgct ggattcctat tattcgacca tcacgacgcg aaccaacagc gtcatgaaag
     1921 tactgacgct gttttctgcg attttcatgc cgctgacttt cattaccggt attttcggca
     1981 tgaatttccg caattttccc gagctcgact ggcattacgg ctttcagggt tccatgatcc
     2041 tcatggtggc agtcatcatc atcatgattt cgatcttcaa gtggaaaaat tggctgtaag
     2101 cgactgagcc ggtcctgcga tcacctccgg acttaatccg cgtcggacac gcgcccacgc
     2161 gcgcgcttca cgatggaacg agtgcgtttc gctgcgatcc tgcgctcttt ggcagcgcgg
     2221 ctcggacgcg tggcaatgcg cttctttggt ttaatcatgg cggcgtgcac cagctcgttc
     2281 aaccgtacca gtgcatcgcg ccggttagct tgctgcgtgc gaaagcggtc ggcatcgatg
     2341 accagaacgt cgagcgcgct gactcgccgt ccgccaaggc ggcgcagtct cgtcttgaca
     2401 tccgcaggca gcgagcggct ggctccgatg tcgaaacgaa gttcgacggc ggaggaaact
     2461 ttgttgacat tctgaccacc cgggcccgac gcgcgcacat gacgaaactc cagttctgcc
     2521 tcgggcactt caaactggcg catcagcacg ctcctttctg ccagcaaggc tgccgcctga
     2581 tgcattgtgc catctggccc gctccaccca aattgccgcg gcgcaccagt cgtgtttgcc
     2641 ggactggcac tgttttgtag gacatcgtga attttccttg acgtgcttgc aatcgaaaat
     2701 gatgaaggct acatttgccg tgggctcccc tatgaacgtc ttcttgaaga gacgaactga
     2761 ataacaatca gaagcagtcg atgtttaatg ggtcaaatcc agtcaccata agtcgtgccc
     2821 atccaccact aatatatcgg gagagagtta tgaaacgatg gacttatacg ggggtcgcga
     2881 aggttctttc ttcaaccgcg gtactcacag cacttttcag caccggtagt ttatttggtg
     2941 ctgatgtcga tggtaaccgt ctgcttaatg cagacaagga acttgaaaaa ggcaactggc
     3001 tcacatacca cggctcgtac aaatcgtggc actacagccc gctcgatcag atcaatacca
     3061 aaaccgttgg caaactttcc gaagcctggt ca
//
LOCUS       DATMZQ010000308         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3017208, whole genome shotgun sequence.
ACCESSION   DATMZQ010000308 DATMZQ010000000
VERSION     DATMZQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017208"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VN496_09960"
     CDS             complement(<1..705)
                     /locus_tag="VN496_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXF17318.1"
                     /translation="MNLTIKNIRARGVQAPLKRPPRSASGEIPNAPLVLIDLQTNEGI
                     TGSCYLFAFMGSMLKPTIAMVEAMAEMITGDALAPFEVEAKLRKRMTLQDSHGIAGQV
                     IAGIDMACWDAYAKGLGVPLVRALGGQVKPVRAYNSCGLWIKDPALLADEASALLQEG
                     GYSALKLRIGRPNFKEDLAAVRNVKKRIGENILLMTDFNQSLTVNEAVIRGRALDDEG
                     LYCIEEPIRHDNYAGCA"
     gene            complement(714..1649)
                     /locus_tag="VN496_09965"
     CDS             complement(714..1649)
                     /locus_tag="VN496_09965"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXF17319.1"
                     /translation="MKIAIIGSGGVGGYFGARLAASGQDVTFVARGAHLEAMQKNGLK
                     IFSALGDVHLPEVKATSDTGIIGPVDVVMIAVKLWATEEAAAAAKPMSGPNTVAVSFQ
                     NGVVAVDTLLPILGKEHVMGGVANIAALIEEPGVIRHNGTMANLFFGELDGKPSARSQ
                     ALQVACKIANVQAEDISDINRAIWEKFVRLVTLSALTSLTRMPIGPLRADPDTRKLMQ
                     QVMEEVVAIGKAKGVKFDADLVADQMTRVETYPANMVASMCGDLRRGNRLELPWLSGM
                     VAKFGKELGVPTPANQFVYAALKLYANGRPAEAQV"
     gene            1808..>2182
                     /locus_tag="VN496_09970"
     CDS             1808..>2182
                     /locus_tag="VN496_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXF17320.1"
                     /translation="MNSPEKLSPAPTRHLASRMDDIEPFHVVVLINRAKELEAQGRSI
                     ANMVVGEPDAPTAPLIAAAGIRAIESGRISYTASLGIAELRDRIAAWYHSRYGVEIPR
                     SRVIVTAGSSGALLLTMGTLLSP"
BASE COUNT          406 a          686 c          609 g          481 t
ORIGIN      
        1 ggcgcagccg gcatagttat cgtggcggat cggttcctct atacagtaaa gtccttcatc
       61 atccagcgcc cggccgcgta tgactgcttc attaactgta agactctggt taaagtcagt
      121 cattagcaat atgttttcgc cgatgcgttt tttgacattc cgcacggcgg ccaggtcttc
      181 cttgaaattc ggacgcccga tgcgcagctt cagcgcactg tagccgcctt cttgcagaag
      241 agcggaggct tcatccgcca gcaatgccgg gtctttgatc cacaggccac agctgttgta
      301 tgcccgcacc ggcttgactt ggccaccgag cgcgcgcacc aacggaacgc cgagcccctt
      361 ggcataggca tcccagcatg ccatgtcgat gcccgcgatg acttgtccgg caatgccgtg
      421 cgaatcctgc aatgtcatgc gcttgcgcaa ctttgcttcg acttcgaacg gggccagcgc
      481 atcgccggtg atcatttcgg ccatcgcttc caccatggcg atggtgggct tgagcatgct
      541 tcccatgaat gcgaacagat agcaacttcc cgtgatgccc tcattggtct gcagatcgat
      601 caacaccagc ggcgcgtttg gtatctcgcc ggacgcggaa cgcggcggac gcttgagagg
      661 tgcctggacg ccgcgcgcgc ggatattctt gatggtcaaa ttcattaggt ctcctagact
      721 tgagcctcgg ccgggcggcc gttggcgtag agtttcaatg ctgcatacac aaactggttt
      781 gccggcgtag gaacgcccag ttccttgcca aatttcgcca ccatgccgga cagccaaggc
      841 agttccaggc ggtttccacg gcgcaggtcg ccgcacatcg acgccaccat attcgcggga
      901 tacgtttcga ctcgggtcat ctgatcggcg acaaggtcgg catcgaattt cacgcccttc
      961 gccttgccga tcgcgaccac ttcctccatg acttgctgca tgagttttcg cgtatcggga
     1021 tcggcccgca gcggcccgat cggcatgcgc gtcaacgaag tcagggccga aagcgtaacc
     1081 aggcgcacga atttttccca gattgccctg ttgatgtcgc tgatgtcttc cgcctgcaca
     1141 ttggcgatct tgcatgcgac ctgtaacgcc tgcgatcttg ccgacggttt gccgtcgagc
     1201 tcgccaaaaa aaaggttcgc catcgtgccg ttgtgccgta tgacgccggg ttcttcgatc
     1261 agcgccgcaa tgttggccac gccgcccatg acatgttcct ttcccaggat cggcagcagc
     1321 gtgtccacgg ccacgacccc gttttggaag gagacggcca ctgtatttgg accgctcatc
     1381 ggtttggcag ccgccgccgc ttcttcggtg gcccaaagct tcaccgcgat catgactacg
     1441 tctaccgggc cgatgatccc ggtatcgctg gtggccttga cctccggcaa atgcacatcg
     1501 cccagcgcgc tgaaaatctt caatccgttc ttttgcatgg cttcaaggtg cgcgccccgg
     1561 gcgacaaagg taacgtcctg cccgctggcc gccaggcgtg cgccaaaata accgcccacg
     1621 ccgccgctgc cgatgatcgc aatcttcatg tacaggttcc cgtatgatcg ttgagtggta
     1681 aaggaccgac atggtagtcg gtcgagccgg atttttgaat cgcgcgttgc tgcggcccgg
     1741 ctgcgcggca taaaatccat tatgcgagcc acactgtcat cgtctcgcgc accgaaaggc
     1801 ttgtgcattg aattccccag aaaagctatc gcccgccccc acgcgtcacc ttgcatcccg
     1861 catggacgac atcgagccgt ttcatgtggt ggtgctgatc aatcgcgcca aggaactcga
     1921 ggcccaggga cgatcgattg ccaacatggt cgtcggcgaa cccgacgcac cgaccgcccc
     1981 attaattgct gcggcgggta tccgcgcgat tgaatccggg cgcatcagct ataccgcttc
     2041 gctcggcatc gccgaattgc gcgaccggat tgccgcctgg tatcacagcc gttacggcgt
     2101 cgaaataccg cgctcgcggg tcattgtgac tgccggatcg tcgggggcat tgttgctcac
     2161 gatgggtact ttgctgtccc cc
//
LOCUS       DATMZQ010000309         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_602656, whole genome shotgun sequence.
ACCESSION   DATMZQ010000309 DATMZQ010000000
VERSION     DATMZQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602656"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VN496_09975"
     CDS             complement(<1..392)
                     /locus_tag="VN496_09975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXF17321.1"
                     /translation="MEYDIVVVGAGPAGLSCAIHLKQLAIERGTETSVCVIEKGSEVG
                     AHILSGAVMDPRALAELFPDWQAKDAPLNAPVTEDRFLFLRENGSFKVPGFLLPACFR
                     NHGNYVVSLGNVCRWLAQQAELLGVEIFP"
     gene            517..936
                     /locus_tag="VN496_09980"
     CDS             517..936
                     /locus_tag="VN496_09980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXF17322.1"
                     /translation="MMKKEEGKLVRVDRVPMRWGEMDALGHMNNVSYFRYFEQARISW
                     FDSLTIDYRPGSEGPILGTMSCKYITPAVYPVDIEVTTYVGKPGRSSFMMFHEMFRAG
                     DPAMLYAEAHAVMVWIDVSQGKSRTLPDWMRKELGGR"
     gene            complement(933..1679)
                     /locus_tag="VN496_09985"
     CDS             complement(933..1679)
                     /locus_tag="VN496_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17323.1"
                     /translation="MFDFKAYRPDQLLLKNRVVMVTGASRGIGRIAALTFAAHGATVV
                     LHGRDVAALKELYDEIERSGYAQPAAVPLDLDKAMSRDYEALAQAIESQLGRLDGILH
                     NASHIEKIGPLEQQSIEEWNRMLRVNLVAPFALTQACVRLLKLAPDAAVIFTLETHGH
                     APAAFWGGYAVAKAGAEALMKIQAAEWDASPNLRANAILPGPVASPSRVKTHPGEVAA
                     SQRRAEDLMPAYLYLIGPDSRGVSGTIVHC"
     gene            complement(1672..>2586)
                     /gene="hpnE"
                     /locus_tag="VN496_09990"
     CDS             complement(1672..>2586)
                     /gene="hpnE"
                     /locus_tag="VN496_09990"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HXF17324.1"
                     /translation="VRFMSWAQRNRFRLAKDTTVAVLLNAKRQPQNVSRYLWHPLCVS
                     ALNTPVAEASAQVFLNVLRDAFNSKRSASDLLLPSLDFSALFPERAARYVQSHRGIVR
                     LGVTVESVLRKDGGFELGPNSERFTHAIVAVSPHRVADLLSSFAELAPISRLIGEITH
                     QPIYSVYLQYAPETKLPFRMGGLESRYAQWLFDRGQLCGQHGLIGVVISASGAHQGLA
                     HDTLARAVHAELEEHFPALGQPRWHQVIAEKRATFSCIPGLLRPDNVTPVANLFLAGD
                     YTASDYPATIESAVRSGVCAAKLVLQNV"
BASE COUNT          578 a          739 c          750 g          519 t
ORIGIN      
        1 ggaaaaattt cgacaccgag caattccgcc tgttgcgcca gccagcggca tacgttgccc
       61 aagctcacga catagttgcc gtgattacga aagcatgccg gcagcaggaa gccgggcact
      121 ttgaaactgc cgttctcccg caggaacaga aagcggtctt cagtgaccgg cgcgttcagc
      181 ggcgcatctt ttgcctgcca gtcggggaac agttccgcca gggcgcgcgg atccatgact
      241 gcgccagaaa gaatatgagc accgacctcg gagcctttct cgattacaca aacggatgtt
      301 tctgtgccgc gctcaatagc aagctgcttg aggtggattg cacaggacag tcccgccggt
      361 cctgccccga ctacgacgat gtcgtactcc attgattcgc gttctgccaa gctattctcc
      421 tcgctaggga cataccgccc tgctgttaga attgcgcgcc gttacgatga taaacggttt
      481 tttacgccgc caaaatgaaa cgcaaaggta agcaccatga tgaaaaaaga agaaggcaaa
      541 ctggtccgcg tggaccgcgt gcccatgcgc tggggagaaa tggatgcact tggccacatg
      601 aacaatgtct cctacttccg ctatttcgaa caggcgcgca tttcatggtt cgattcgctt
      661 acgatcgact atcgtcccgg ctcagaaggg ccgatcctcg gcaccatgag ctgcaaatac
      721 atcacgccgg cggtctaccc ggtcgatatc gaagtcacga cttacgtcgg aaagccgggg
      781 cgcagcagtt tcatgatgtt ccacgaaatg ttccgggcgg gcgatccggc gatgttatac
      841 gccgaggcac atgccgtgat ggtgtggatt gacgtgagtc agggcaagtc caggacattg
      901 ccggactgga tgcgcaagga attgggcggg cgttagcagt gcactatggt gcctgaaacg
      961 cctcggctat caggtccgat caaatagaga taggccggca ttaggtcttc ggcgcggcgc
     1021 tgtgacgcgg cgacttcgcc cggatgtgtt ttcacgcggg aaggggaggc aaccgggcct
     1081 ggaagtatcg cattggcgcg caaatttggt gaagcgtccc attccgccgc ctggattttc
     1141 attagagctt ccgcccccgc ctttgcaacg gcataacctc cccagaacgc ggccggtgca
     1201 tgcccatggg tctcgagggt aaaaatgact gccgcatcgg gagcaagctt caacagccgc
     1261 acgcatgctt gcgtcaaagc gaacggggcc accaaattga cccgaagcat gcggttccat
     1321 tcctcaatgc tctgctgttc gagcggccca atcttttcga tatgcgatgc gttatgcagg
     1381 atgccatcga ggcggcccaa ttgcgattct atggcttgag ccaaggcctc gtaatcgcgt
     1441 gacattgcct tatccagatc cagcggaacc gccgctggtt gcgcataacc gctgcgttcg
     1501 atttcatcgt acagttcctt gagcgctgcg acatcgcgtc cgtgcaatac gactgtcgcg
     1561 ccgtgcgcgg caaacgtcag cgcggcaatg cggccgatac cccggctggc cccggtgacc
     1621 ataacaacgc gatttttgag caggagttgg tcaggacggt acgccttgaa gtcaaacatt
     1681 ttgcaatacc agcttggcgg cgcacacgcc gctgcgaacg gcagattcaa tggtggccgg
     1741 gtaatctgat gccgtatagt cgccggccaa gaaaaggtta gccaccggag ttacattgtc
     1801 cggccgcaac aggcccggta tgcaagagaa ggtggcgcgc ttttccgcaa tgacctgatg
     1861 ccagcgcggc tggccgagcg ccggaaaatg ctcttcaagt tccgcatgca cggcacgtgc
     1921 gagtgtgtcg tgcgcgagtc cctggtgcgc gccgcttgcc gatattacga ccccgatcaa
     1981 accgtgctgg ccgcacagct gtccacgatc gaaaagccac tgtgcgtaac gggattcgag
     2041 accgcccatg cggaaaggta actttgtttc aggcgcgtat tgcaggtaca ccgaatagat
     2101 cggttgatgc gtgatttcgc caatcagtcg gctaatgggc gccagttcgg caaagctgga
     2161 cagcaaatcg gccacacgat gcggtgaaac ggctacgatc gcatgggtaa aacgctcgct
     2221 gttgggcccc aactcgaaac cgccatcctt tcggagcaca gattccacgg taacgcccag
     2281 acgcactatg ccacggtgcg attgcacgta gcgtgccgcg cgttccggga acagcgcgga
     2341 aaagtccagc gagggtaaca acaggtcgct tgcactccgc ttactattaa acgcgtcacg
     2401 cagaacattc aggaatacct gggcagaagc ttccgctacc ggagtattca aggccgaaac
     2461 gcatagcgga tgccacagat aacgcgatac gttttgcggt tgccgtttgg cattcagcaa
     2521 aacggctacg gtcgtatcct tggcaaggcg aaagcggttg cgctgcgccc aggacatgaa
     2581 gcgcac
//
LOCUS       DATMZQ010000310         4851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4617545, whole genome shotgun sequence.
ACCESSION   DATMZQ010000310 DATMZQ010000000
VERSION     DATMZQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4617545"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..1704
                     /locus_tag="VN496_09995"
     CDS             508..1704
                     /locus_tag="VN496_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXF17325.1"
                     /translation="MRFWWRAGTWLWLIFATLMAQAADGRPGIHEAIVAGDYYGAGEQ
                     ASPAGPVEGDAFIVSPYLNLTQPVKGDALLAGGGVLISAPVAGNLYGMGGSVLLESPI
                     RGNGRLTGGRVEIGALGTVAGKTTLVGGRVSVLGKAGQQLTVFAEHVTLDGEVDGNVT
                     IAARTLVIGPHTRVKGRLTYRGSQPAQSDPAAVIIGGMNYLSFDFEEETYQPVAKVVA
                     WVGAIAFTAGLFLIGMLAILLAPGWTEHMSRLGRRRPMSSLGLGLVVIVCVPIAVVLL
                     MLTIVGIPLALMLLMAWPMILLLGYLAGVMAVSDAIAGPRAEAKGRRVFLLAMGLGVM
                     LLFARVPVAGWAIGMLLVILGIGAMALNAMGAVAPVRIKKQTPVAPEAGKSELASRQE
                     PTFRRE"
     gene            1722..2435
                     /locus_tag="VN496_10000"
     CDS             1722..2435
                     /locus_tag="VN496_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17326.1"
                     /translation="MMNRTKKAVFAIAVLLVAVLAGCAITKKNQQSKMDDDISRIVTL
                     PDGTTCMEPAGLADSREKPGAIQLRELLKSDVTTVDAVAKATALKLQPDEAEAVYFDA
                     CRAYAKGTIQKDEFEANRTVYVALRGKFTVEGVKEWQDKKDGIADPGKLCLVSLPDTD
                     PDHRSFTRVVPANSSVSDCAELAVMNGSREILLGCTKGHWENTWAKKPISLGTAGTAV
                     KQLTAKGTALAPDPDCGWN"
     gene            complement(2455..3429)
                     /locus_tag="VN496_10005"
     CDS             complement(2455..3429)
                     /locus_tag="VN496_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17327.1"
                     /translation="MFRRMIVVAFAPLWAACSAVAPEDPDLTPMNPTAVRETRIVSTG
                     IQEFATFTSTNVTYTRPKMQRREYSMHPKSGLPASDNDPQVRIDLLERKIAWRLDQKA
                     KKAIECPLQGCIAPATRKLPPGKLPEQDKTRDPNCRLKIGNPVLRVVSTGAKRNINGF
                     DTEQYNISWLVTLRDNASRKSTGTVSVDLWTAPVTLGMDEAIALERTYARAHDKILAA
                     GSEARPGLLPYEVDTLLNAYLAQSATSADRANLLAAMSKVDEAKGYPILMRVKWTVAG
                     AACSTDETPTGAGDKPLFVLTWEVKSEKLLPLHDSLFAPPKGYKIRKG"
     gene            3668..4120
                     /locus_tag="VN496_10010"
     CDS             3668..4120
                     /locus_tag="VN496_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXF17328.1"
                     /translation="MFKSIHAVIDFLALHAAGPHSGAQECSGTITVDEALKAEEARFK
                     AQMSGDGAAMNKLFGEDLVYIHSSTVVDTKKSFIESITSGNVKYRSMTRGESIVRTYG
                     AVAIVSGDAKFQVTVKGEDRMLDLLYHAIWAKRPAGVQFVSWQATKKG"
     gene            4261..>4851
                     /locus_tag="VN496_10015"
     CDS             4261..>4851
                     /locus_tag="VN496_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HXF17329.1"
                     /translation="MTMPTEPIGSIPRPIGLIEAVNKRGNGDDPALDALYEEAIRDTV
                     QRFEATGSPVITDGEQRKYQNFWTYSVHGLRNTATDGFRIPFSAGHTRRMLRLTEGPF
                     RYKRYADSYLEVAQRYAHVPVKQAIISPSALSLMYPADGIPGYSRDQFIQDLLLEHET
                     EIRRCLKKGAHKVQIDFTEGRLAMKVDPSGQLLSSFI"
BASE COUNT         1001 a         1346 c         1479 g         1025 t
ORIGIN      
        1 accctttcgt ccgtcgcatg ggtgccatta aacaactttg cggcgtcgca caatagacgg
       61 aaaaactcca tatgttctat tccgatttgg aatgtaactc gctgaagggt gcgagcgaag
      121 cgaggtctga agtgccgggc gaaaagttaa gtgattgatg tagtgctaga tgtttgatat
      181 ttatattctg atgtcacggc gatccgcatt ccagttatgg gtatttcggt atccgagata
      241 gctgcaatgc gtgtccacca agcccgcaac ggcgcaggcg atcctgattt tacggtgcct
      301 tgcgacattc ttaaggaccc tacggtgcct aacggtggcg cacgcggcat aatgcgcgac
      361 acgagtcgat atgcggaagg ccaccccggc tgttgggccg cgcacggaga gcagcgtctc
      421 cgggcctctg cggaattcgc agcgacacta ttcagaccga tttatggatg aaatcctttc
      481 tttgcagttc gtagggtggc tggcggcatg aggttttggt ggcgcgcagg aacatggcta
      541 tggctgatct ttgccacgct catggcccag gcggctgacg ggcgcccggg aatacacgaa
      601 gcaatcgtcg ccggcgacta ttacggagca ggcgaacaag catcccctgc cgggcccgtg
      661 gaaggtgatg ctttcattgt ttcgccttat ctgaatctga cccagccggt taaaggcgat
      721 gcgctactag cggggggcgg agtgttgatc agcgcgcccg tggccggcaa tctgtacggc
      781 atgggcggca gcgtgttgtt ggagtctccc attaggggta atggccggct taccggcgga
      841 cgcgtcgaga tcggcgcact cggaacggtt gccggcaaaa ccacgctcgt gggcggccgt
      901 gtgtcggttc tgggcaaagc cggccagcaa ctgaccgtgt tcgcggagca tgtcacgctg
      961 gatggtgagg tcgatggcaa cgtcaccatc gcggcgcgca cgctggttat cggaccacac
     1021 accagggtaa agggcaggct gacctatcgc ggctcgcaac cggcccagag cgatcctgcc
     1081 gctgtcatta tcggcgggat gaattacctg agcttcgatt tcgaagagga gacgtatcag
     1141 ccggtcgcga aagtcgtggc ctgggtcggt gcaatagcat ttacggctgg cctgttcctc
     1201 atcggcatgt tggcaatcct gctggcgcct ggctggaccg aacacatgag ccggctcgga
     1261 cgccgcaggc cgatgtcgtc gcttggcttg gggctggtgg tgattgtctg tgtgccgatc
     1321 gcagtggtgc tgttgatgct taccatcgtg ggtattccgc ttgcgctgat gttgttgatg
     1381 gcctggccaa tgatcctgct gttgggatac ttggccggcg tcatggcggt gagcgatgca
     1441 attgcgggcc cgcgtgcaga agcgaaagga cgtcgtgttt tcctgctggc catggggctg
     1501 ggcgtcatgc tgctattcgc gcgggtgccg gttgcaggct gggccattgg catgctgctg
     1561 gtaatcctgg gtattggagc aatggcgttg aatgccatgg gcgcggtggc gccggtcagg
     1621 atcaagaagc agacgcccgt tgcaccggaa gctggcaaat ccgaattggc ttcgcgtcag
     1681 gagcccacgt ttcgcaggga atagcgtttt cgcatagagg aatgatgaac agaacaaaaa
     1741 aagcggtgtt tgcaatcgcc gtgttgctgg ttgcggtact ggccggatgc gcaattacca
     1801 agaagaacca gcaatcaaag atggacgacg atatcagccg tatcgtgacg cttcctgatg
     1861 gcacgacttg catggaacct gccggactgg ccgatagccg cgaaaaaccc ggcgcgattc
     1921 aactgcgcga attactcaag tcggatgtca caaccgttga tgccgtggcg aaggccacag
     1981 cgctcaaact gcagcccgac gaagccgaag cggtctactt cgatgcctgc cgcgcttacg
     2041 caaaaggcac gatccagaag gatgaattcg aggcgaaccg aacggtatat gtcgccttgc
     2101 gcgggaaatt tacggtcgaa ggcgtgaagg aatggcaaga caagaaagac ggcattgctg
     2161 atcccggcaa gctgtgcctg gtcagcttgc ccgataccga tcctgatcac cgcagcttca
     2221 cgcgtgttgt acccgccaat tccagcgtta gcgattgcgc cgaactggcc gtgatgaatg
     2281 gcagccgaga aatcctgctc ggttgcacca agggacactg ggaaaataca tgggcgaaga
     2341 agccgatttc gctcggcacc gcgggaaccg cggtgaagca attgactgca aaaggcaccg
     2401 cccttgcgcc cgatccggat tgtgggtgga actgaaagcc gtcccgcctt tgcttcatcc
     2461 cttcctgatc ttgtatccct tggggggcgc aaacagcgaa tcatgcaacg gcaagagctt
     2521 ttcggacttc acttcccagg tcaaaacgaa tagcggtttg tccccggctc cagtgggcgt
     2581 ctcatcggtg gagcaagcgg cgcccgcgac tgtccacttc actcgcatca gaatggggta
     2641 gccctttgcc tcatcgacct tgctcatggc ggcgagaagg tttgcgcggt ccgctgatgt
     2701 ggcgctttga gccaggtaag cgttcagcag cgtatccact tcatagggca acaatcctgg
     2761 ccgtgcttca ctgcccgcgg caagaatctt gtcgtgcgcc ctggcatacg tcctttccag
     2821 ggctatcgcc tcgtccatcc ctagcgtgac gggcgccgtc cacaagtcga cgcttaccgt
     2881 gccggtggat ttgcgagaag cgttgtcgcg cagcgtgaca agccagctga tgttgtattg
     2941 ctcggtgtcg aaaccgttga tgtttcgctt cgccccagtc gagactactc ttagcaccgg
     3001 gttgccgatc ttcaaccggc aattcggatc gcgcgtcttg tcctgctcgg gcaatttgcc
     3061 aggaggtaac ttgcgcgtag ccggagcgat gcagccctgc agaggacact caatcgcttt
     3121 tttcgccttt tggtccagtc tccacgctat cttgcgttcc agtaagtcga tgcggacttg
     3181 cgggtcgttg tccgaagcgg gtaaaccact cttgggatgc atgctgtatt cccggcgctg
     3241 catctttggc cgggtatagg tgacattcgt gctggtgaac gtggcgaatt cctggatgcc
     3301 tgtgctgacg atccgcgttt cccgaaccgc ggtcgggttc atgggcgtca ggtcaggatc
     3361 ttcaggcgca accgcagagc aggcggccca aagcggcgcg aatgcaacga cgatcatgcg
     3421 gcgaaacatg aagggtgggg ctcccaacaa ttgggataaa acacacccag tgtaactgca
     3481 gccgggtcaa cgcgcgtatc tcgtgcgcta ggtatcaggg cgagccaagg cgttctccga
     3541 gtgaaagcta aatcaaggta tccttccgtc gcggcggttt tggttcgttt ttgatcgtag
     3601 tcctgacgtc gtctctggac catctggcaa cgcgcaatat ccataacaac ttacaacgga
     3661 ggactcaatg ttcaagtcga ttcacgcagt catcgatttt ctggcgttac acgcggccgg
     3721 tccgcattcc ggtgcacagg aatgcagcgg gactatcacg gtggatgaag cgctcaaggc
     3781 cgaagaggcg cggttcaagg cccagatgag cggcgatggc gctgcgatga acaaattgtt
     3841 cggcgaagac ctggtctaca tccactccag taccgtggtc gataccaaga aaagcttcat
     3901 cgaatccatt acctcgggaa atgtgaagta ccgctccatg acgcgcggtg aatcgatcgt
     3961 ccggacctat ggcgccgtcg caatcgtcag cggcgatgcc aaatttcagg tcaccgtcaa
     4021 aggggaagac cgcatgctgg acttgctgta tcacgccatc tgggccaagc ggcccgccgg
     4081 cgtgcagttc gtgtcctggc aggcaacgaa gaaaggctga tctgccgtaa cgccgcggac
     4141 gaagtccgcg gtgcggccga tgcggtccgg ccgatgcggc tgaaccgcat cccttcggct
     4201 catggcatac acgcgcggcc gcggtttcct gcgatagaat aaatcgccaa ggcgactgct
     4261 atgaccatgc ctaccgagcc catcggcagc atcccgcgac caataggtct catcgaggcg
     4321 gtgaacaagc gcgggaatgg cgatgatccg gcgctcgatg ccctttacga agaggcgata
     4381 cgggacaccg tgcagcgttt cgaagcaacc ggctcacctg tcatcaccga cggggaacaa
     4441 cgcaaatatc agaacttctg gacctactcg gtccatgggc tgcgcaatac ggccacggac
     4501 ggcttcagaa ttccattttc ggccggccat acccggcgaa tgctgcggct gaccgaaggc
     4561 ccgttcagat acaaacgcta cgccgacagt tatctcgaag tcgcgcagcg ttacgcgcat
     4621 gttccggtca agcaggccat catttcccca tcggcgctga gcctcatgta tcccgcggat
     4681 ggtattccgg gatattcacg cgaccagttc atccaggatc tgctgctcga acacgaaacc
     4741 gagatacggc gctgtttaaa gaagggcgcg cacaaggtgc agatcgattt caccgaaggg
     4801 cgcctcgcca tgaaggttga tccttccggg caactgctgt ccagcttcat c
//
LOCUS       DATMZQ010000311         7615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4271816, whole genome shotgun sequence.
ACCESSION   DATMZQ010000311 DATMZQ010000000
VERSION     DATMZQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4271816"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1919
                     /gene="secD"
                     /locus_tag="VN496_10020"
     CDS             57..1919
                     /gene="secD"
                     /locus_tag="VN496_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXF17330.1"
                     /translation="MNRYPLWKYVLIAVALVVGLLYTLPNFFGEVPAVQISPAKTTAK
                     IDETLLSRVDLALGQANIKPDGIFLDPASIKVRFPNTDMQLKAKDVLQGSLGENYVVA
                     LNLLSSSPRWLSRINALPMYLGLDLRGGVHFLLQVDMKGALTKAADRYAGDIRTTLRA
                     KEIRYAGIARDGQNVVVKFRDPETREKALTELTKQNPDLLLNPIDVSSEYSISAALKP
                     EVQKRIQEFAIQQNITTLRNRVNELGVAEPIVQQAGSDRVVVQLPGVQDTAKAKDILG
                     RTATLEVRMVDEEHSDPASLQAAIAGQTPLGDELLYDREGAPHILKREVILTGDRISD
                     AQPGFDSQTGEPAVHINLDGRGATIFQQITRENIGKRMAMVLVEKGKGEVVTAPVIRS
                     EIPGGRVQISGRMTTTEARDVALLLRAGALAAPMEIVEERTVGPSLGADNITRGFHST
                     MIGFAAIAIFMMAYYLFFGVVSVIALAVNLMLLVALLSMLQATLTLPGIAAIALTLGM
                     AIDSNVLINERIREELRNGTTPQAAITAGYERAWATILDSNITTLIAGIALFMFGSGP
                     VRGFAVVHCLGILTSMFSAVLVSRSIVNLIFGARRKLDKLPIGNTNWYKRQTAA"
     gene            1952..2881
                     /gene="secF"
                     /locus_tag="VN496_10025"
     CDS             1952..2881
                     /gene="secF"
                     /locus_tag="VN496_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXF17331.1"
                     /translation="MEFFRIRKDIPFMRHALVFNIISLITFVLAVIFLSTRGLHLGVE
                     FTGGTVMEVNYGHTVDVDKIRDTLTRVNLADATVQSFGSSQTALIRLPAKQGVTGAQL
                     SETVIKALRQDDSSVELRRVDFVGSQVGKELVENGALALLLVSIGIVVYLWMRFEWKF
                     GLSAIVANLHDVVIILGFFAFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRIRENFRK
                     MRKASTPEVINNAITRTMSRTIITHGCTQLMVTSILIFGGETLHYFALALTIGILFGI
                     YSSVLVASPLVMWLGVTREDFIKPEKKQEEAVV"
     gene            2889..3527
                     /locus_tag="VN496_10030"
     CDS             2889..3527
                     /locus_tag="VN496_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXF17332.1"
                     /translation="MELLSQFLEVILHLDLHLRELAQTYGAWIYGILFLIIFCETGVV
                     VTPFLPGDSLLFVAGTIAAAGHMNIHALALLLTAAAIAGDSLNYSIGRYLGPKVFRFD
                     ESRFFKRAYIDRTRAFFDRHGGKAIILARFVPIVRTYAPFVAGIGAMQYSRFLMFNVT
                     GAVLWVVLLSYAGFFFGNLPVVRDNLTAVILGIIFLSIMPGAVELWRAKQAR"
     gene            complement(3531..4904)
                     /gene="purB"
                     /locus_tag="VN496_10035"
     CDS             complement(3531..4904)
                     /gene="purB"
                     /locus_tag="VN496_10035"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXF17333.1"
                     /translation="MNPSSVLTALCPLDGRYSEKVSALSDYFSEFALIRYRVRVEVEW
                     LKALCATRALDGIPTLSPATVMQLDALVRDFTVLDGEAVKQIELRTNHDVKAIEYWLK
                     DKLSDNRDIADITRFVHFACTSEDINNLCHALMLRDALTHVLLPIIDALIERLAALAH
                     STASLPMLSRTHGQPATPTTLGKEMANFVHRLRRQRARLAGVSLTGKMNGAVGNFNAH
                     FAAFPAFDWETFCRHFVEKLGFEFNAYTTQIEPHDSIAELFDAFAGFNTVLLDFDRDV
                     WGYISIGYFKQKVKEGEVGSSTMPHKVNPIDFENSEGNLGLSNALLSHLASKLPISRW
                     QRDLTDSTVLRNMGVALGYGLLAYDACLRGLGKLEPDPKRLADDLSANWEVLAEAIQT
                     VMKRHSIDDAYEQLKELTRGKGSITRDALQVFIRSLSIPPEEKERLLALTPVTYVGLA
                     ETLAKRL"
     gene            complement(4918..6021)
                     /gene="mnmA"
                     /locus_tag="VN496_10040"
     CDS             complement(4918..6021)
                     /gene="mnmA"
                     /locus_tag="VN496_10040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXF17334.1"
                     /translation="MNKKQRIVVGMSGGVDSSVAAWLLKQQGYDVVGLFMKNWEDDDD
                     DEYCNSRQDLIDAVSVADIIGIDIEAVNFSAEYKDRVFNSFLAEFKAGRTPNPDVLCN
                     AEIKFKSFLDHALQLGADRIATGHYAQVRQVDGLFQLLKAEDGTKDQSYFLYRLNQAQ
                     LASTLFPLGKMYKREVRELAARLKFPNHDKKDSTGICFIGERPFREFLNRYLPSDPGD
                     MVDLDGNRVGSHAGLMYYTLGQRQGLGIGGRSEGAGEPWYVCRKDMTANRLVVVQGHD
                     HPALLAQALTAGELSWISGTNPHCNWVYGAKTRYRQRDAPCEIERVDSTSCEIRFAEP
                     QWAVTPGQSVVVYESTLCLGGGIIDAAIPYLAP"
     gene            complement(6018..6482)
                     /locus_tag="VN496_10045"
     CDS             complement(6018..6482)
                     /locus_tag="VN496_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXF17335.1"
                     /translation="MIDMIWKPDVTVAAVIEREGRFLLVEEHTERGLLFNQPAGHLEA
                     DESITAAVARETLEESAWDFLPEQLIGVYRWQSSDSITTYLRFAFSGRLGAHHPDRAL
                     DRGIVRTTWLAADEVRATRARHRSPMVVRCMEDYLAGKRTPLDVMVDLGFAR"
     gene            complement(6479..7111)
                     /locus_tag="VN496_10050"
     CDS             complement(6479..7111)
                     /locus_tag="VN496_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HXF17336.1"
                     /translation="MDEWTDLIKLTVALLAIVDPVASIPVFLGTTTGYAPAERQRVTQ
                     VVAITVFCVLGIAALIGTQVLNFFGISIPSFLIAGGILLLLMAIAMLQARESGIRQTP
                     DEAAEAAERDAVAVVPLGIPLLAGPGAISTMIIATHQSHGVVHRLMLLIPAAVIAIAV
                     WWTFAAATRISARLGTTGMNIITRVMGLIIAAIGVEYIYRGITELFPKLL"
BASE COUNT         1650 a         2070 c         2119 g         1776 t
ORIGIN      
        1 cagtccccgc agggcttgcc tgtcacccct gaatatccgc accgcaaagg gcacacatga
       61 atcgctatcc gctctggaaa tacgttctca tcgccgtcgc cctggttgtc ggtctgttgt
      121 acacgttgcc gaattttttc ggcgaagtgc cggcagtgca gatttcgccg gcgaaaacca
      181 cggccaagat tgatgaaacc ctgttgtcgc gcgtcgatct tgcgctcggt caggcgaaca
      241 tcaaacccga cggcattttt ctggatccgg ccagcatcaa agtccgattc cccaacactg
      301 atatgcagtt gaaggcgaag gatgttctgc aagggtcctt gggcgagaac tatgtcgttg
      361 cactgaacct gctttcgagt tcgccacgct ggctctcccg gatcaatgca ttacccatgt
      421 atctgggcct tgacttgcgt gggggcgtgc acttcctgct gcaggtggac atgaaaggcg
      481 cgctcactaa agctgctgat cgctacgctg gggatatccg tactactctg cgggccaagg
      541 agatccgcta tgctgggata gcgcgtgacg gccaaaacgt cgtggtcaaa ttccgggacc
      601 cagaaacacg agagaaggct cttacggaac tcaccaaaca gaacccggac ttgctactga
      661 atccgattga cgtgtcgtct gaatactcga tcagcgcagc gctaaaaccg gaagtacaaa
      721 agcgcattca agagtttgcc atccagcaga acatcacgac cctgcgcaac cgggtcaatg
      781 aactgggcgt cgccgaaccg atcgttcagc aagcggggtc ggaccgggtg gtggtgcagc
      841 ttcccggtgt acaggacacc gccaaagcga aggacattct gggacggaca gcgaccctcg
      901 aagtgcgcat ggtggatgaa gagcatagcg atccggcgtc actacaggca gcgatcgcag
      961 gacaaacacc gcttggcgac gaacttcttt acgatcgcga aggagcgccg cacatactca
     1021 aacgcgaggt catcctgacg ggagaccgta tttccgacgc tcaaccgggc tttgacagcc
     1081 aaaccggaga accggcggtc cacattaacc tcgatgggcg gggtgccacg attttccagc
     1141 agatcacgcg cgagaacatc ggcaagcgca tggcgatggt cttggtggaa aaaggcaaag
     1201 gcgaagtagt caccgcgccg gtgattcgat cggaaattcc cggcggccgt gtacagatca
     1261 gcggacgcat gacgacgacc gaagcacgcg acgtggcgtt gttgttgcga gcaggagctc
     1321 tggctgcccc aatggaaatc gtggaagagc gaacagtagg cccaagcctg ggtgcagaca
     1381 atatcacccg cggcttccat tcgaccatga tcggcttcgc ggcaatagcc attttcatga
     1441 tggcctatta tttatttttc ggcgtggttt cggtgattgc cttggcggtg aacctgatgc
     1501 tgctggttgc gctgctttcc atgctgcagg caacattgac cctgccgggc attgcggcca
     1561 tcgcactgac gcttggtatg gcgatcgact ctaacgtgct gatcaacgaa cgtatcaggg
     1621 aggaattgcg caacggaacc acgccgcaag ccgctattac tgccggttac gagcgggctt
     1681 gggccacgat cctggattcg aacataacga cgttgattgc cggcattgcc ttattcatgt
     1741 tcggtagcgg cccggtgcgt ggttttgcag tggtccactg cctcggcatt ctgacttcaa
     1801 tgtttagcgc ggtgctggtt tcgcgcagca tcgtgaacct catttttggt gcgcgtcgca
     1861 agctcgacaa actgccgatt ggcaatacca actggtacaa acggcaaacc gccgcataaa
     1921 agcggatcac tgtcggaagc aaggaacaaa aatggaattt ttccgtatcc gtaaagatat
     1981 cccttttatg cgccacgcgc tggtgttcaa catcatttcg ttgatcactt tcgtgctcgc
     2041 tgtaatattc ctgtctacga ggggtctgca tctcggcgtt gaattcacgg gcggcaccgt
     2101 aatggaagtg aattacggcc acaccgtgga cgtggacaag attcgcgata ccttgacccg
     2161 agtgaacctc gcggatgcga cggtgcaaag tttcggcagt tcgcaaacgg cactgatcag
     2221 gctgccggca aaacaaggcg taacaggcgc ccagttgtcc gagaccgtga tcaaggcgtt
     2281 gcgccaggat gattcctcgg ttgaactgcg tcgcgtggat ttcgttggtt cgcaggtcgg
     2341 caaggaactg gttgagaatg gtgccttggc actattgctc gtatccatcg gcatcgtggt
     2401 ttatctctgg atgcgtttcg aatggaaatt tggattgtcg gccatcgtcg ccaatttgca
     2461 cgatgtcgtg atcatcctgg ggttctttgc gttcttccag tgggagtttt ctttgccggt
     2521 gcttgcggcg gtactggccg tattgggtta ttccgtcaac gaatcggtcg tggtgttcga
     2581 ccggattcgc gaaaattttc gcaagatgcg caaggcctct acgcctgaag tcatcaacaa
     2641 cgccattacc cgcacgatgt cgcggaccat cattacgcac ggctgtaccc agctgatggt
     2701 gacctcaatc cttattttcg gtggtgagac gctgcattat tttgcgctcg cactcacgat
     2761 cggcattctg ttcggcattt actcatcggt tcttgtcgcg agcccgttgg tgatgtggct
     2821 tggtgtgacg cgagaagatt tcatcaagcc ggaaaagaag caggaagagg cggtggtctg
     2881 attttgcgat ggagctgctt tcgcagtttt tggaagtaat tctccatctc gacctgcacc
     2941 tccgggaact ggcgcagact tacggcgcgt ggatatatgg aattctgttt ctcattattt
     3001 tctgcgagac cggtgttgta gttacccctt tcctgcctgg cgattcgctg ctgtttgtcg
     3061 cgggcaccat cgcagccgca ggtcacatga atatccacgc tctggcgttg ctgctgactg
     3121 cggcagccat tgctggggat tcgttgaatt attcaatcgg ccgctatctg ggaccgaaag
     3181 tgttccgatt cgatgaatcc cggttcttca aacgtgccta tatcgatcgc actcgcgcat
     3241 tcttcgaccg gcacggcggc aaggcgatca ttcttgcccg ctttgtaccg atcgttcgga
     3301 cgtacgctcc gttcgtagcc gggattggag ctatgcagta ctcgcgcttt ttgatgttca
     3361 atgtgacggg ggcggttctc tgggtggtgc tgctgtctta tgccggcttc ttttttggta
     3421 accttcctgt agttcgcgac aatcttacag ccgttattct tggcatcatt ttcttgtcca
     3481 tcatgcccgg tgcggtggaa ttatggcgcg cgaaacaagc gcgctagaca tcagagccgc
     3541 tttgcaagcg tttccgccaa tccgacatag gttaccggtg tcaaagccag cagccgctct
     3601 ttttcttccg gcggaatact cagactgcgg ataaacactt gcaaagcgtc ccgcgtgatc
     3661 gagcccttcc cacgcgtcaa ctccttcaat tgttcatagg catcgtcaat gctgtggcgt
     3721 ttcattactg tctggatggc ttcggccaat acttcccagt tggcactcaa atcatcggcg
     3781 agccgcttcg gatccggctc caatttacca aggccacgca agcaggcgtc gtatgcaagc
     3841 aatccgtatc cgagggcgac acccatgttg cgtaaaacgg tagagtcggt caggtcgcgt
     3901 tgccaacggg atatcgggag tttcgaggct aagtgcgaga gcagtgcgtt cgataagccg
     3961 agattgcctt ctgagttttc aaaatcgatc ggattgactt tgtgcggcat ggttgaggac
     4021 ccgacttctc cctcttttac tttctgcttg aaatagccaa tcgaaatata accccagaca
     4081 tctctatcga aatcgagcag gacggtattg aatcccgcga atgcatcgaa tagctccgcg
     4141 atgctgtcgt gcggttcgat ttgagtggta taggcattga attcaaaacc aagtttctcg
     4201 acgaagtgcc ggcagaacgt ttcccagtca aaggctggga aggccgcgaa atgtgcattg
     4261 aagttaccga cggctccgtt catttttccg gttagcgaaa cgccagccaa gcgggcgcgt
     4321 tgccgcctga gccggtgtac gaaattagcc atctccttgc caagagtagt cggggtcgcc
     4381 ggctggccat gcgtgcgcga cagcatgggg agggaggcgg tggaatgggc cagtgccgct
     4441 agtctttcga tcagcgcatc gattatgggc aaaaggacgt gtgtcagagc gtcgcgaagc
     4501 atgagggcat ggcaaagatt attgatatcc tcggatgtgc aagcaaaatg cacaaaacgc
     4561 gtgatgtctg cgatatctct gttgtctgaa agtttgtctt tcagccagta ttcgatcgct
     4621 ttgacgtcat gattcgtgcg aagctcgatt tgcttcaccg cttcgccgtc gagaaccgtg
     4681 aagtcgcgaa ccagtgcatc cagttgcatc accgtagccg gagaaagcgt cgggatcccg
     4741 tctagtgccc gtgtggcgca aagagccttc agccattcga cttcgacgcg aactcgatag
     4801 cggatgaggg cgaattcgct gaagtaatca gatagtgcgg aaaccttttc actatagcgg
     4861 ccatcgagtg ggcacagcgc ggtaagaacg gaggaagggt tcatcgaagg aactctatca
     4921 tggcgcgaga taagggatcg ccgcatcaat gatgccgccg ccgagacaga gagtggactc
     4981 gtaaacgaca acggactggc ccggcgtgac ggcccattga ggttcggcaa aacgtatttc
     5041 gcatgatgtg ctgtcgaccc gctcaatttc gcacggcgca tcccgttgcc ggtatcgggt
     5101 cttggcgccg tatacccaat tgcagtgcgg atttgtcccg ctaatccacg acagttctcc
     5161 agccgttagc gcctgcgcca gcaatgccgg atgatcgtgg ccttgcacca cgacaagtcg
     5221 attggctgtc atatccttgc gacagacata ccaaggctcg ccagcgcctt cactgcgacc
     5281 gccgatgccc aatccctggc gctgaccaag cgtgtaatac atcagaccgg catggctgcc
     5341 gactctgttt ccgtccagat cgaccatgtc accggggtcg ctcggcaaat accggttcag
     5401 gaattcacgg aaaggccgtt cgccgataaa gcagattccg gttgagtctt ttttgtcgtg
     5461 attcggaaat ttcagtctgg cggcgagctc tcgcacttcg cgcttgtaca ttttgccaag
     5521 ggggaaaagg gtgctcgcca actgtgcctg gtttagccga tacaggaaat agctttgatc
     5581 cttggtaccg tcctcggcct tgagcaactg aaacaagccg tccacctggc gcacttgcgc
     5641 gtaatgtcca gttgctatgc gatcagcgcc cagttgcagt gcgtggtcga gaaaagactt
     5701 gaacttgatt tcggcattgc acaaaacatc cggattcggt gttcgcccgg ccttaaactc
     5761 ggcgagaaag ctgttgaaca cgcggtcttt gtactccgcg gaaaaattca ccgcttcgat
     5821 gtcgattcca atgatatccg cgaccgaaac ggcgtcgatc aagtcctggc gcgagttgca
     5881 gtattcatca tcatcgtcgt cctcccaatt cttcatgaac aggccgacca cgtcatagcc
     5941 ttgctgcttt agcagccagg cggcgaccga agagtccact ccgcccgaca tgccgacgac
     6001 gatgcgctgc tttttattca ccttgcgaac cccaggtcca ccatgacatc aagtggtgtg
     6061 cgcttgccgg ccaggtagtc ttccatgcag cgtacgacca tcgggctacg atgccttgcg
     6121 cgggtcgcgc gcacttcgtc cgctgcgagc catgttgtac gcacgattcc gcgatccagg
     6181 gctctgtccg ggtgatgtgc tccaagccgg ccggaaaacg caaatcgaag ataagtggtg
     6241 atactgtcag atgattgcca tcgatagacg ccgatgagct gttctggcaa gaaatcccat
     6301 gcgctttctt ccagtgtctc cctcgcaact gccgcggtga tcgattcgtc agcttcgagg
     6361 tggcccgctg gctgattgaa cagcagtcca cgttcggtat gttcttcaac gagcagaaag
     6421 cgtccttcgc gctcgatcac tgcagcaaca gtaacgtcag gtttccagat catatcgatc
     6481 agagcagctt cggaaacaac tctgtaattc ctcggtaaat gtattcgaca ccgattgcgg
     6541 caatgatcag ccccattaca cgcgtaatga tgttcatgcc ggtcgtgccc aatcgggccg
     6601 atatgcgcgt tgcagcggca aacgtccacc acactgcaat tgcgatgacg gcagctggga
     6661 taagcagcat aagtcgatgg acaacgccgt gagactggtg tgtcgcgatg atcatggtac
     6721 tgattgcacc gggcccggcc agcaggggga tgcccagcgg gacgactgcc acggcgtctc
     6781 gctcggctgc ttcggccgct tcatcaggag tctggcggat gcctgattcc cgcgcttgca
     6841 gcattgcaat tgccatcagc aacagcagta tgccgcccgc aatcaggaaa gaggggatgc
     6901 tgatgccgaa gaaattcagc acttgcgtac cgatcaatgc cgcgatgccc agtacgcaga
     6961 aaacagtaat tgccacaacc tgcgtgacgc gctggcgttc cgcaggagcg tagccagtag
     7021 tggtacccag gaatacggga atactggcaa ctggatctac aatcgccagc agcgcaactg
     7081 tcagcttgat aaggtcggtc cattcatcca cggcttacac cttcaattca ctagcagcgc
     7141 acgaaataaa aaaggcaggg atttgacccc gccttgcatc ggatcggctc cggattattt
     7201 ggccttgtcc tcaataacgt tttccggccg ttttgtgtct ggaacagggc cgatgttcgc
     7261 acctggttga gcttgtgcca agccaacctt ggcagacgct acgccattca cagcggcagt
     7321 aggaaggagt cccaaacgta gtggatggat gcaaagatcc cttttatagc tcgtcccggt
     7381 ttatacctca ggcagcgagt actaaatcgt tcactagtcc gcaagacgtt ggcaagaata
     7441 aggatttccg ccagtcccgt caaacgaggt tcgacaacga atcatcttac gctcgggcgc
     7501 tttcacgaaa aaaggcaggg aaatctccct gccttttgaa ttgcaactgg gtaacgttta
     7561 ctgcttcgcg gcggccggct ttgcgtccgc agccggagcc gcggtttcct tcttg
//
LOCUS       DATMZQ010000312         4603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3019450, whole genome shotgun sequence.
ACCESSION   DATMZQ010000312 DATMZQ010000000
VERSION     DATMZQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3019450"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..2199
                     /locus_tag="VN496_10055"
     CDS             397..2199
                     /locus_tag="VN496_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXF17337.1"
                     /translation="MTPNRDVISPEIARTLDGLFQERVRRSAKAVAYRSFDEPGVWRD
                     YSWEKVAELVARWQDGFEADGLQEGDRVAIMLRNSVNWIVFDQAAMGLGLVNVPLYTS
                     DRPDNTAYILHDSGAKVLVLENTDQWAMFADVQSQLGGLKRILCVNALPGPATDPRIK
                     SVADWLPKSTGAVRHVNQDGTKLTSIIYTSGTTGKPKGVMLSHKNMLENAASALQCFD
                     VTTQDLFLSFLPLSHTLERTAGCYLTMMAGACVAYARSIPQLGEDLVAIRPTIMISVP
                     RIYERVYATIKARLAEGPPLRRKLFEYAVEVGWARFEHQQGRGPWKAAFLLWPLLDRL
                     VARKVMARLGGRLRAAVSGGAALAPDIARLFVGLGLPILQGYGLTETSPVVSTNRLDD
                     NVPASIGKPIPGVQVKLGEKDALLVKGSNVMLGYWNLPDATKAMFTADGWLNTGDTAR
                     FDKEGRLYITGRLKEIIVMSTGEKIPPVDMEAAILQDSLFEQVMILGEGRPYLSAFVV
                     LNPDQWRKSALQHALPETVESISGNRSAETFLLDCVQKQIKSFPGYAKINRIAVMREP
                     WTIENGLLTPTMKLKRAKVVESHRKEFESLYAGH"
     gene            2186..2611
                     /locus_tag="VN496_10060"
     CDS             2186..2611
                     /locus_tag="VN496_10060"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXF17338.1"
                     /translation="MPVIDHKPAHQQTASLPIKREPTLRVVPMPSDANYTGDIFGGWI
                     MSQVDIAGSIPAIEVAKGRVATVAVNSFIFKQPVMIGDLVSFYANVVRTGRTSITVDV
                     EVYARRRTLHEVVKVTEATLTYVAVDDERRPRPLEQGSG"
     gene            2663..3988
                     /locus_tag="VN496_10065"
     CDS             2663..3988
                     /locus_tag="VN496_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HXF17339.1"
                     /translation="MHKEHQTSLIALAVAWAVAGISSPASAAGFALIEQSASGMGNAF
                     AGAAATAEDASTIFYNPAGMSKLQGTQVTIGAHVIDLSAKLNNSGSSISLGAQAIGSQ
                     PGGDGGDAGGVSVVPNLYFVMPIGDRLNFGIGINAPFGLVTEYDQNWVGRFQGIKSEL
                     TTYNINPALSFKVNDTFAIGAGVNYQHLDANLTNAVLLPVPVEGRAEVDANDDAWGWN
                     IGVLFQPSPSTKIGASYRSKLEYKLEGTTTVNTVTGVPIPQASGATTADVTLPDSFSL
                     SLAQRLNEQWEFLADATFTRWSEINRINIVNSTNGTLRDSLLLDFDDTWRYSIGANYK
                     LNEKWTLKGGVAYDQTPVKSATTRSVRLPDTDRTWLSIGAGLKVQQSGKLDFAYSHLF
                     LKDAEINSTRSQQVPGLSTPTPAPGTASTVAGTYTGSIDIFSVQYTMSF"
     gene            4078..>4603
                     /locus_tag="VN496_10070"
     CDS             4078..>4603
                     /locus_tag="VN496_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXF17340.1"
                     /translation="MARPVNSAVRKVAVLGAGVMGSQIAAHLVNAGVEAILFELPAKE
                     SDPNGNVRKAVDSLKKLEPSPLSVSVRADYIQAANYDQDLGLLKDCDLVIEAISERMD
                     WKKNLYEKVAPHLGESAIFASNTSGLSINSLSEAMPAHVRSRFCGVHFFNPPRYMHLV
                     ELIAGRAADPAILDR"
BASE COUNT         1087 a         1196 c         1298 g         1022 t
ORIGIN      
        1 actatgccat cacggagcgc atgcagcgcc aggtaatgtt cgccgccaag cgcagccttg
       61 agttgctcga gttggccttg ttgagttcga ctcaattggt accagcctcg aagcgatgat
      121 cgattgcacc tctggcagtg ccaaaggtgc cgaagagcaa gcggggccag gctcgcgata
      181 gactggctcc gtttttttac atctggatta ggcgatgcgg ttaaaaaggt tcgatcgctc
      241 tgacgaacat ggttctgaac gcggcataga agttcagtgg tacggcagat gaaatggtag
      301 attatgaatt agggatgtat ggacactggt atcgtaacta tactcaggga tgtttctcca
      361 ttgaatggat agccagacca tttcagaagc aaggccatga cgcccaacag agatgtcatt
      421 agcccggaga tcgctcgcac tctggatggg ttatttcagg aacgcgtgcg ccgttccgcc
      481 aaggcggtcg cgtacagaag tttcgatgag ccaggtgtct ggagagacta tagctgggaa
      541 aaagtcgcag agctggtcgc gcgttggcag gatggattcg aagccgatgg tttgcaagag
      601 ggggaccggg tcgcgatcat gctgcgcaat agcgtcaatt ggatcgtttt cgaccaggcg
      661 gcgatgggtc ttggcctggt aaacgtgccg ctctatacgt ctgaccgtcc cgacaacacc
      721 gcgtacatct tgcacgattc aggcgcgaag gttttggtat tggagaatac cgaccagtgg
      781 gccatgttcg cggatgtgca aagccagctg ggaggactca agcggatcct gtgcgttaac
      841 gcgctccctg gtccggcgac cgatccacga atcaagagtg ttgctgactg gttgcccaag
      901 tccacgggcg cggttcggca cgtcaatcag gatggcacga aactaaccag cattatttat
      961 acgtccggga ccacgggaaa gcccaagggc gtcatgcttt cgcacaaaaa catgctcgaa
     1021 aatgcagctt cggcattgca atgtttcgac gtcactactc aagacctatt cctgtccttt
     1081 ttgccgttgt cgcatacgct tgagcgaacc gcgggatgct atttgacgat gatggccggt
     1141 gcctgtgtgg catacgcacg ctccattccg caattgggcg aagacctggt tgcgataagg
     1201 ccgaccatca tgatttccgt tccacgaata tacgaacggg tttacgcaac cataaaggcc
     1261 cggctcgcgg aagggccgcc gcttcgcaga aaattattcg agtacgcggt tgaagtgggt
     1321 tgggcaagat ttgaacatca acaaggcaga gggccatgga aagctgcgtt cctgctgtgg
     1381 ccgctgctgg atcggctcgt cgctcgcaag gtcatggcgc gtttgggtgg gagactccgc
     1441 gcagcggtca gcggcggtgc ggcactcgct ccggatattg cgcggctgtt tgtgggcctt
     1501 ggcctgccga ttcttcaggg atatggactt acggaaacca gcccggtagt cagcacaaac
     1561 cgcctggatg acaacgttcc agccagtatc ggcaagccga ttcccggggt gcaggtcaaa
     1621 ttgggcgaga aggatgcact gctggtcaag ggttcaaatg tcatgcttgg ttactggaat
     1681 ttaccggatg ccaccaaagc aatgttcacc gcggatggat ggcttaatac tggcgacaca
     1741 gcccggtttg ataaagaagg gcgtctctat atcaccggac gccttaagga aattattgtc
     1801 atgtcgaccg gggaaaaaat accgcctgtg gatatggaag ccgccatcct gcaggattcg
     1861 ctgttcgagc aggtcatgat tttgggcgaa ggaagaccgt atctcagcgc ctttgttgtg
     1921 ctcaatccgg accaatggcg aaaatcggct ctccagcatg cgctgccgga gacagtggaa
     1981 agcatatcgg gcaaccgctc agcagagact ttcctgctgg attgcgtcca gaaacagata
     2041 aaatctttcc cgggttacgc aaaaatcaac cgtatagccg taatgaggga gccctggaca
     2101 attgaaaacg gcctccttac acccacaatg aaactgaaac gcgcgaaagt tgtggaatcg
     2161 cacaggaaag aattcgaaag tctctatgcc ggtcattgac cacaaaccgg cccaccagca
     2221 aacggcatcg cttcccatca agcgggagcc aacattgcgt gtcgtgccca tgccgtcgga
     2281 tgcgaactac accggggata tctttggcgg atggatcatg tcgcaagttg atattgctgg
     2341 cagcattcct gccatcgagg tcgcgaaagg acgtgttgct accgtggcgg tcaattcgtt
     2401 catattcaag caaccggtga tgattggaga tttggtgagc ttttatgcga acgtggtaag
     2461 aaccggccgt acgtcaatta ccgttgacgt ggaagtgtat gcccgccggc gtacattgca
     2521 tgaagtcgtg aaagttaccg aagcaacttt gacctatgtg gcagtcgatg atgagcgccg
     2581 cccgcgtcca ctcgagcagg gatcaggctg aagaattgtg tcacttgaca tggcggtttt
     2641 ttatttcaac aaggagaggt aaatgcacaa agaacaccaa acaagcttga ttgcactagc
     2701 cgtcgcatgg gcggtagcag gcatatcttc tccggcctcg gccgctggat ttgcactgat
     2761 cgagcaaagt gcaagcggaa tgggaaacgc gttcgctgga gcggcggcta cagcagagga
     2821 tgcaagcacc attttttaca atcctgccgg tatgtcgaag ttgcagggaa cacaggttac
     2881 gatcggtgcc catgtaattg acttgagcgc aaaactcaac aactcgggct cttcaatttc
     2941 gttgggtgcg caagctatag gcagtcaacc cggtggcgac gggggagacg caggaggcgt
     3001 atcggttgtg cccaacttgt atttcgtgat gccgattggc gatcggctta attttggtat
     3061 cggaataaat gcgccgttcg gcctcgtgac ggagtacgat caaaactggg taggacgctt
     3121 tcagggaatt aaatcggagc taacgaccta taacatcaat cccgccttgt ctttcaaagt
     3181 aaacgatacc tttgccattg gtgctggagt gaattatcag catcttgacg cgaatctcac
     3241 gaacgctgtg cttcttccag tgccggtcga aggtcgcgcc gaagtcgatg ccaacgatga
     3301 tgcatgggga tggaacatag gtgtcctttt tcaaccttcg ccctccacca aaatcggcgc
     3361 aagctatcgg tcaaagctcg aatacaagct ggaaggcaca acgactgtca ataccgtcac
     3421 cggcgttcct attccgcagg ctagtggagc aaccaccgcg gacgtcaccc tgccggattc
     3481 tttttcgtta agcttggcgc aacgcctgaa cgaacagtgg gagttcctcg cagacgctac
     3541 cttcacgcgt tggagcgaaa tcaatcgaat caatatcgta aattccacga acggaacctt
     3601 gcgcgacagt ctgcttctgg atttcgatga cacttggcgg tattcaatcg gtgcgaacta
     3661 caaattgaac gaaaagtgga ccctcaaagg cggcgttgct tacgaccaga ctccagtcaa
     3721 gagcgccact actcggtcgg tacggctgcc ggataccgat cgcacctggc tatcaattgg
     3781 tgccggtctg aaggtgcaac aaagcggcaa actggacttt gcctattcgc atcttttcct
     3841 gaaagacgct gaaatcaact ccacgcgcag ccaacaagtg ccgggccttt ctacgccgac
     3901 gccagcacca ggaaccgcat caaccgtggc cggcacgtac accggatcta ttgatatttt
     3961 cagcgttcag tacaccatga gcttctagat ctggccggtg gcgggcatct ccccgccacc
     4021 ggttttcatt tccggcttgg tggaacgcgg ccacgccgtt gcaactctaa agacaacatg
     4081 gcacgcccag tcaattctgc cgtccgaaaa gtcgccgttc ttggcgccgg agtcatgggt
     4141 tcccagattg cagcccacct ggtcaacgct ggagtggaag cgatcctgtt tgaattgcca
     4201 gcgaaggaga gcgatcccaa cggcaacgta cgcaaggccg ttgatagcct gaaaaagctg
     4261 gaacccagtc cgctttccgt gtccgtccgt gccgactaca tacaggcggc aaattacgat
     4321 caggacctgg gactacttaa agactgcgac ttggtcatcg aggccatttc cgagcgcatg
     4381 gactggaaaa aaaacttgta cgagaaagtt gctcctcatc tcggcgaaag cgccatattt
     4441 gcgagcaaca cgtccgggct ttccatcaat tccctgtccg aagcgatgcc cgcgcatgtg
     4501 cgctcgcgtt tttgcggcgt gcattttttc aatccgccgc gctacatgca tttggtggag
     4561 ttaatcgccg ggcgcgcggc cgatccggcg attctggacc ggc
//
LOCUS       DATMZQ010000313         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4964187, whole genome shotgun sequence.
ACCESSION   DATMZQ010000313 DATMZQ010000000
VERSION     DATMZQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4964187"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VN496_10075"
     CDS             complement(<1..552)
                     /locus_tag="VN496_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF17341.1"
                     /translation="MGQPLQDPRAEREAYYRRIAPAHLTPLWEMMSALVPREPHSPCV
                     PALWKYAEVRPWLMESGSLITAKEAVRRVLILENPGLHGQSAITNSLYAGWQLILPGE
                     VAPSHRHTQTALRLIVEGTGAYTAVDGERVTMHPGDFIITPSWTWHDHGNAAQEPVVW
                     LDGLDIPMVRFFDAGFSEDYPQDE"
     gene            complement(578..1285)
                     /locus_tag="VN496_10080"
     CDS             complement(578..1285)
                     /locus_tag="VN496_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HXF17342.1"
                     /translation="MSTTTATLAVKTAAELAQQPQEYQTAVKKIVISHAVNELYGAQV
                     FDEPAIALAPTPYAKWLTCRVAMEEYHHHVRFRALAEQIGVPAQMMDPKIKKPLTIFG
                     FELKTWPEFCVIKAIADFAEILQVEDLLHCSFHPLRDLARITMPEEKFHAKFGKDFCT
                     ELIRTEEGKAAVQDAINRYFPITPGFFGASNSKNNEMYRRWALKSRSNDEMREDFVAR
                     VTELVEKDFGLQLPKIH"
     gene            complement(1297..2229)
                     /locus_tag="VN496_10085"
     CDS             complement(1297..2229)
                     /locus_tag="VN496_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17343.1"
                     /translation="MSPDQSLSGRVALVTGGARGIGLAIVEELQRHGALVLIADSGVS
                     ISGGQPDPAVAESIAAKLGAGAKPFCEDLTQAGAPERAVQAALAAYGAIDIVINNAAI
                     IRDAFIFKASRENWDQVLATNLSAPFALLAAATPHMREQQKNGRAPGRIVNIVSSAGL
                     FGNFGQAAYASAKAGLMGLTRVVAMDMSRSKVTCNAIAPFAATRVTESIQPANEAQMT
                     YKQRALGIPAQYVAKLAVFLSSNANDVTGQLFGVRGRELMLFSQPRPVARSLMPAAGA
                     WEEAALAQAIKVELAAGMTDLATDLEFFNTEPIV"
     gene            complement(2226..>3153)
                     /locus_tag="VN496_10090"
     CDS             complement(2226..>3153)
                     /locus_tag="VN496_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXF17344.1"
                     /translation="IMELEYSEHALSAEERSRGLVLACRSQVWGDVVVRRLQAEDFIV
                     HPSRVLRCRVTDLTPLTHDILRLRMEIVAGGPFTFSAGQFASLEFPVAAGAVRDYSMA
                     NCPHEKVLEFHIRVMPAAHSVSRRVATLLRPGDMVKVSGPLGTSYLRSQHAGEMLCVA
                     GGSGLAPIKSIIETALHDGFAQPMHLYFGVRSERDIYFELELAELQRRFPSFKAHIVL
                     SEPGTQHDSMQQRGVEAGATLLPRRYGLVTNAVAADFAALAGFKTYFAGPPPMVDAAS
                     ALARERGVQLRDIHADAFFPAAVESKPAASVQ"
BASE COUNT          629 a          971 c          930 g          623 t
ORIGIN      
        1 ttcgtcctgc ggatagtctt cggagaaacc ggcgtcgaag aagcgaacca tcggaatgtc
       61 caacccgtcg agccagacta ccggctcctg cgctgcattg ccgtggtcgt gccaggtcca
      121 ggacggcgtg atgatgaaat cgcccggatg catggtcacc cgttccccat cgactgcggt
      181 gtaggcaccg gtgccttcga cgatcaaccg caacgccgtt tgggtgtggc gatggcttgg
      241 cgcaacttcg ccaggcagga tcaattgcca gcctgcataa agcgaattcg tgatcgccga
      301 ctggccgtga agacccggat tttcgagaat cagcacgcgg cgcaccgcct ctttcgcggt
      361 gatcagactg cctgattcca tcagccacgg acgcacctcg gcatacttcc acaatgccgg
      421 gacacaaggc gaatggggtt cgcgcggcac cagcgcgctc atcatttccc atagtggagt
      481 gagatgcgcc ggcgcaatgc gccggtaata agcttcccgc tccgcgcggg gatcctgcaa
      541 aggttggccc atattgcctc cagctttgct gcctgatcta gtgaatcttt ggcagttgca
      601 aaccgaaatc tttctctacc agttcagtaa cgcgcgcaac aaaatcttca cgcatctcat
      661 cgttgctgcg gctcttcagt gcccagcgcc ggtacatttc gttattcttg gagttactcg
      721 caccgaaaaa tcccggcgtg atcgggaagt agcggttgat tgcgtcctgc acggcagcct
      781 tgccctcttc agtgcgaatc agttcggtgc agaaatcctt gccgaatttg gcatggaatt
      841 tctcctccgg catggtaatg cgggccaggt cgcgcaatgg atgaaaggag cagtgcagca
      901 gatcttccac ctgcaggatt tcggcaaagt cggctatcgc ctttatcaca cagaattccg
      961 gccatgtctt caactcaaag ccaaagatcg tcagcggttt cttgatcttc ggatccatca
     1021 tttgcgccgg cacgccgatc tgctccgcca gcgcacgaaa gcgcacgtgg tgatggtact
     1081 cctccatggc cacgcgacag gtcagccatt ttgcatacgg ggtcggcgcc agcgcgatgg
     1141 caggttcgtc gaaaacctgc gcaccgtaca gttcgttcac cgcgtggctg atgacgattt
     1201 ttttgaccgc ggtctggtat tcctgcggct gttgcgcaag ttcggccgca gttttaacgg
     1261 cgagcgtagc agtagtggtg ctcatgagtt gatccctcag acaatgggtt cggtattgaa
     1321 aaattccaga tcggtggcaa ggtcggtcat gccagccgcc agttcgacct tgatggcttg
     1381 cgcaagtgcc gcttcttccc aagccccagc tgccggcatc aggctgcgcg cgaccgggcg
     1441 cggctgggaa aacagcatca attcgcggcc gcgcacacca aatagttgtc cggtcacatc
     1501 gttggcattc gaactcagga ataccgccag tttggcaaca tactgtgccg gtatgccaag
     1561 cgcgcgctgc ttgtatgtca tctgggcttc attggctggc tggatcgatt cagtcacccg
     1621 ggtcgcggca aacggcgcga tggcattgca tgtgactttc gagcgggaca tgtccattgc
     1681 gacgacgcga gtgagtccca tcaggcctgc cttcgccgag gcataggcgg cttgtccgaa
     1741 attgccgaac aatccagcgc tggacacaat gttgacaata cgtccgggcg cacggccgtt
     1801 cttctgttgt tcgcgcatgt gcggagtcgc cgcggccagc agagcgaaag gagcactgag
     1861 attggtcgcg agtacctgat cccagttttc ccggctggct ttgaaaataa atgcgtcgcg
     1921 gataatcgcg gcattgttga tgacgatatc gatcgcgcca taagccgcca acgcggcctg
     1981 cacggcacgt tccggcgcgc ccgcctgcgt caggtcttcg cagaacggct tcgcacctgc
     2041 gccgagtttt gcggcgatcg attccgcgac cgccggatcg ggctgcccgc cggaaatact
     2101 cacaccgcta tcggcaatca gcaccagcgc accgtggcgc tgcagttctt cgacgatcgc
     2161 caggccgatg ccgcgcgcgc cgccggtgac cagagcaacc ctcccggaca ggctttggtc
     2221 cgggctcatt gcacgctcgc tgcgggtttg gactcgaccg ccgccgggaa aaaagcgtcg
     2281 gcatgaatgt cgcgcagttg cacgccgcgc tcgcgggcaa gcgcactcgc cgcgtccacc
     2341 atcggcggcg ggccggcaaa atacgtcttg aacccggcca gggcggcaaa gtctgccgcg
     2401 accgcattgg tgacaaggcc gtaccttcta ggcaatagcg tcgcgccggc ttcaacgccg
     2461 cgctgctgca tcgagtcatg ctgcgtaccg ggctcggaca gcacgatgtg agctttgaac
     2521 gatggaaagc gtcgctgcaa ttcggccagt tccagttcga aatagatgtc gcgctcggaa
     2581 cgcacaccaa agtaaagatg catcggctgg gcaaagccgt catgcaaggc ggtttcgata
     2641 atcgatttga tgggtgccaa tccgctgccg ccggctacgc acagcatctc gccggcatgt
     2701 tgcgagcgca ggtatgaggt gcccagcggg cccgacacct tgaccatgtc gcccggtcgg
     2761 agcaacgtcg ccacgcggcg gctcacggaa tgcgctgcag gcatgacgcg gatatggaac
     2821 tccagcacct tctcgtgcgg acaattcgcc atcgaataat cacgcactgc accggccgcg
     2881 accggaaact cgaggctggc aaactgcccc gccgaaaaag tgaacggccc gcccgcgacg
     2941 atctccatcc ggagccgcag gatatcgtgc gtgaggggcg tcaggtcagt cacccggcag
     3001 cgcaatacgc gcgaaggatg cacgatgaag tcctcggcct ggagccggcg cactaccacg
     3061 tctccccaca cctgtgaccg gcaggcgagc acaagaccac ggctgcgctc ctcggcggac
     3121 aaggcatgct ccgaatactc gagctccatg atg
//
LOCUS       DATMZQ010000314         6923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2202376, whole genome shotgun sequence.
ACCESSION   DATMZQ010000314 DATMZQ010000000
VERSION     DATMZQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202376"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VN496_10095"
     CDS             <1..255
                     /locus_tag="VN496_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXF17345.1"
                     /translation="AQGAELHAQGQILRQASAQVKIADTVGAGDASIGGLLFSLITHG
                     ASDWQNHLRWAVAAGSAACMQSGAVPPPLAAIESVLATMQ"
     gene            260..1459
                     /locus_tag="VN496_10100"
     CDS             260..1459
                     /locus_tag="VN496_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HXF17346.1"
                     /translation="MPDFRSREFLLDHIRHTMAFYHPHAIDPRGGFFHFFRDDGTVYD
                     PHTRHLVSSTRFIFNYAMGFRQFGEPAYLRAVEHGMAYLRDHHRIASTGGYAWLLDGQ
                     RVADGTNHCYGLAFVLLAYAHASMAGVEQARAWIGETFDLMEHHFWSSTHGLYADEAT
                     PDWSVVSSYRGQNANMHACEALLAAFDATRDTRYLHRAEILAHNITVRQAHLADGLIW
                     EHYRADWSVDWDYNRNDSGNLFRPWGFQPGHLAEWAKLLLILERHAPQLASTQRETQW
                     FVPRAAALFDAAVTHAWDDQHGGLVYGFAPDGRVCDGDKYFWVQAESLAAAALLAQRT
                     GEDKYWQWYERIWDYSWKHLVDHEFGAWYRILTRDNRKYSDEKSPAGKTDYHTMGACY
                     EVLNVLT"
     gene            complement(1492..2655)
                     /locus_tag="VN496_10105"
     CDS             complement(1492..2655)
                     /locus_tag="VN496_10105"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HXF17347.1"
                     /translation="MNRLPALRFALCGMLALAIAIGVGRFAFTPILPMMQKDYAVTLR
                     LAGLLASANYAGYFMGALSAIWIRISVATIVRVSLVSLALLTAAMAAIHEPLAWLLLR
                     FLAGMISAWVFVFASAYSLQQLAKHGSARFSGIVFGGVGLGIALTGSLCLLFLRWSWS
                     ADRAWTTAGVLALLLTLACWSGYAGAGQAPASASKAAVAPLRLGRHLVMILCYAMLGF
                     GYIIPGTFLPAMAKQVILDPAVFGWAWPIFGVAALVSTLIAGGLSAHVPNRLVWSMSH
                     VVMAIGVAMPVVSPGMAGIVVAALCVGSTFVVATMAGMQEARVAAPEHASQLMATMTA
                     AFGLGQIIGPLLVSLVANEHNGMNALLIVVALLLLVSAFEIARGSPKQIAQKK"
     gene            2794..4482
                     /locus_tag="VN496_10110"
     CDS             2794..4482
                     /locus_tag="VN496_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF17348.1"
                     /translation="MHDLVIRNGTVVDGTGAARRVADVAIDGELITAVGREIARGHRE
                     LDANGLLVAPGYVDIHTHYDGQATWDPYLTPSSWHGVTTVVFGNCGVGFAPVRKGAAP
                     YLINLMEGVEDIPGTVLSEGVKFNWESFPDYLDALEAAPKVIDVGAQVPHAALRFYVM
                     GDRGADHAAAPNDTEIGQMGILLEQALRAGALGFTTSRTTKHRARDGRLTPSLSAREP
                     ELFGLAAAMKRAGRGVIEVNSDFGPGEFDILRDAAQIAGRPLSVLLLQVNNAPELWRE
                     TRAQIHVARAAGLDVNGQVGCRPIGVMMGLETTIHPFSSHPAWLEMSSLNPAQRVTRL
                     RQSVELRRQLVEERPADEHTKRIADAFGRIYVVDDNYDYEPDPSLSIGARAKTTGTSP
                     WALALDAMMACEGKQLLVETFENYTEGNLDNIREMLVDEATVMGIADGGAHVCTVCDA
                     SSPTFLLTHWARDRKRGEQLPLEFLIRKQTRDCALAYGLKDRGVLAAGYRADLNIIDF
                     ARLGMRKHEVVYDLPAGGRRLMQRADGYRHTFVAGVETLCDDQHTGAMPGKLVR"
     gene            4490..4912
                     /locus_tag="VN496_10115"
     CDS             4490..4912
                     /locus_tag="VN496_10115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXF17349.1"
                     /translation="MSKIFRVEYPIRFSHCDPAGQVFFPRFFDMLHDAMEDWFNTGLQ
                     ERFSDLLMNQKLGTPTVGTQCDFISPARFGDTLTIELSILRLGNSSIELAFNASIDGR
                     ACLKCRHTICMFSKDTAKAVPIPEALRNRMQDFVVPAK"
     gene            4972..5427
                     /locus_tag="VN496_10120"
     CDS             4972..5427
                     /locus_tag="VN496_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17350.1"
                     /translation="MSRDILKLTLLTLFLALVLAGAPMSFAADAGKTTGADVGRKMDE
                     TAETIKNYSVEQRDEALKNAKNLLAEADGRIEQLENDMSKNWDEMNASARKHARDTMR
                     TLRRQRQDLSQSYGELKRSSANAWDEVKGGFARSYDALHDSFAKAAKEF"
     gene            5553..6044
                     /locus_tag="VN496_10125"
     CDS             5553..6044
                     /locus_tag="VN496_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXF17351.1"
                     /translation="MNNIAMATRKPELSSGAGSEWSVACTGARLRRLTRRVTAHYEHH
                     LRPAGLKLSQYSLLVHLSEKPESLSTLADRMEIDRTTLSRNLQPLLSRGLVDETSGSD
                     GRQRLFVLSANGARLRASARKQWRQAQLALEKHLGSDFVANLHTQLEQALLRLKPALP
                     EEN"
     gene            6047..>6923
                     /locus_tag="VN496_10130"
     CDS             6047..>6923
                     /locus_tag="VN496_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17352.1"
                     /translation="MARSSEAHSYWFIVLLAAALLMVTMGSRQSQGLFLSPLNTSTGL
                     GIATISLAMAIGQFVWGAVQPIAGAIADRYGPGRVLAGGILLLALGSALVPFMTSSAG
                     LIFSLGVLVAAGSGAGSFSVLIGSASRHLPAEKRGMAAGIINAGGSFGQFVFAPITQK
                     LISALGWMGAMWSIAAIALAALPITRSLRGKPASATEQARSIASNPSDKGMRVALREA
                     LKDRSYLLLHAGFFTCGFHIAFLVTHLPGEVQLCGLPAQVASWSLGIIGLSNIAGSLL
                     AGFGTQRYRSKYILFW"
BASE COUNT         1433 a         2028 c         2069 g         1393 t
ORIGIN      
        1 gcgcaaggcg ctgaactgca tgcgcaggga cagattttgc ggcaggcttc tgcgcaagta
       61 aaaatcgccg acacggtggg cgctggcgac gccagtatcg gcggattgct attcagcttg
      121 atcacccatg gcgcttcaga ctggcaaaac catcttcgct gggcggtcgc agcgggaagc
      181 gcggcatgca tgcagtccgg cgccgtgccg cccccattgg ctgcaattga aagcgtactc
      241 gcgacaatgc aataaccgca tgccggattt ccgtagccgc gaatttctac tcgatcacat
      301 ccgccacacg atggcgtttt atcatccgca cgccatcgat ccccgcggcg ggttttttca
      361 tttcttccgt gacgacggca ctgtctacga tccgcacacg cgccacctcg tcagcagcac
      421 gcgcttcatt ttcaactatg ccatgggctt caggcagttc ggtgaacctg cttacctgcg
      481 cgccgtagaa cacggcatgg cttatttgcg cgaccatcat cgaattgcct caacgggcgg
      541 ctacgcctgg ttgcttgatg gccagcgagt cgccgatggc accaaccatt gctacggcct
      601 ggccttcgtg ctgctggcct acgctcatgc atccatggcc ggtgttgaac aggcgcgcgc
      661 gtggattggt gaaacatttg acctgatgga gcaccatttc tggtcgtcga cacatggcct
      721 ctacgcagac gaagccacac cggactggtc ggttgtatct tcgtaccgtg gacagaacgc
      781 caacatgcat gcttgcgagg cgttgttggc ggcattcgat gccacgcgcg acacacgcta
      841 tttacatcgt gcggaaattc tggcgcacaa catcacggtg cggcaggcac atctggcgga
      901 tggactgatt tgggagcact accgcgccga ctggtcggtg gattgggatt acaaccgcaa
      961 cgacagtggc aatctgtttc gcccgtgggg ttttcagccg ggacaccttg cagaatgggc
     1021 gaagcttctg ctgattctgg agcgtcacgc gccgcaactg gcctcaacgc agcgagagac
     1081 ccaatggttc gttcctcgcg ctgctgcgct gttcgatgcc gcagtaaccc atgcatggga
     1141 cgatcagcac ggtggtcttg tttacggttt cgcaccggac gggcgcgtgt gcgacgggga
     1201 caagtatttc tgggtgcagg cggagtcgct cgcggccgcg gcgctgctgg cacagcgcac
     1261 cggtgaggac aaatactggc aatggtacga acggatttgg gactacagct ggaagcattt
     1321 ggtcgaccat gaatttggtg cctggtaccg catcctcacg cgagacaacc gcaaatatag
     1381 cgacgagaaa tccccggcgg gcaaaacgga ctaccacacc atgggcgcat gctacgaagt
     1441 gctgaacgtg ctgacctgac gcttcggtgc aatagcactt gcccgaacag gttatttttt
     1501 ttgcgcgatt tgttttggcg agccacgcgc aatttcgaaa gcactgacca gcagcaatag
     1561 ggcgacgact atcagcagtg cgttcatgcc attgtgctca ttggccacca ggcttacgag
     1621 cagcgggccg atgatctgtc caagaccgaa tgcggccgtc atagtcgcca tcaattgcga
     1681 agcgtgttcc ggagcggcca ctcgcgcttc ctgcatgccg gccatcgtgg ccacgacgaa
     1741 ggtgctgccc acgcaaagcg cggcgacgac aatcccggcc atgcccggcg aaaccaccgg
     1801 catggcaacg ccaatcgcca tgacgacgtg cgacatcgac cagaccagac ggttgggtac
     1861 atgggcagat agtccgcctg caatcagcgt ggacaccagt gccgctacac cgaatatcgg
     1921 ccacgcccaa ccgaacaccg ctggatcgag gatcacctgt ttggccatgg cgggtaaaaa
     1981 tgtgccgggg atgatgtagc cgaagccgag catcgcgtag cagaggatca tcacaagatg
     2041 tcgccccagt cgtagaggcg ctacggccgc cttgctcgca gacgcaggtg cctggccggc
     2101 gccggcatac cccgaccagc atgccagcgt gagcaggagc gcgagcacgc ccgccgtagt
     2161 ccacgcccgg tctgccgacc aagaccaacg caaaaacagc aggcacaggg atccggtgag
     2221 cgcaatgccg aggcccactc cgccaaacac gatgccactg aaccgcgcgc ttccatgctt
     2281 ggccaactgc tgcaggctgt atgcggaagc aaacacaaac acccacgcgc tgatcatgcc
     2341 ggccagaaag cgcagtagta accaggccag tggctcatga atcgcggcca tggccgcagt
     2401 aagcaatgca aggctcacga gggaaacacg cacgatcgtt gcaacgctga tgcgaatcca
     2461 gatggcggac agcgcaccca tgaaataccc ggcatagttg gcggacgcga gcaaccccgc
     2521 caggcgcaat gtcactgcgt agtccttttg catcatgggc aggatcggtg taaaggcaaa
     2581 gcggcccacg ccaatggcaa tggccaaagc cagcatgccg cacagagcga atcgcaacgc
     2641 gggcagtcgg ttcacatcag ttttctgaaa aggctggacc ggatgttatc agcgccgacg
     2701 ccgggttagc gcaggcgcga agcccggact ataaggtttc atgcgaacat agaggggttc
     2761 tggcctttac ccagaatcac ccggagaaag ttcatgcacg atctggtaat ccgtaacggt
     2821 acggtcgttg atggcactgg cgccgcgcgc cgtgttgccg atgttgcgat agacggcgaa
     2881 ttgattaccg ccgtcggtcg tgaaatcgcc cgcggacacc gtgaacttga tgccaatgga
     2941 ctgcttgtcg cgccgggcta tgtcgatatc catacgcatt atgacgggca ggccacgtgg
     3001 gacccgtatt tgacgccgtc ttcctggcat ggcgtgacaa ccgtggtctt tggcaattgc
     3061 ggtgtcgggt tcgccccggt acgcaaaggt gctgcgcctt atctcatcaa tttgatggag
     3121 ggtgtggaag atattcctgg caccgtgctg tccgaaggcg tgaaattcaa ttgggaatcg
     3181 tttccggatt atctagatgc gctggaagct gcgccaaaag tcatcgacgt cggtgcgcag
     3241 gttccgcacg cggccttgcg tttttatgtc atgggggacc ggggtgccga ccatgcggcc
     3301 gcaccgaacg acaccgaaat cggacagatg ggcatcttgc tggagcaggc tttgcgcgcc
     3361 ggtgccctgg gattcacgac ctcgcgcacg actaaacacc gcgcacgcga tggacggctc
     3421 acgccaagtc tttctgcgcg cgaaccggaa ttgtttggcc tcgctgccgc catgaagcgc
     3481 gcaggtcgcg gcgtcattga ggtcaattcc gatttcggcc ccggcgaatt cgatattctg
     3541 cgcgacgcgg cgcaaatcgc cggacggccc ttgtcagtat tgctgctgca ggtcaataac
     3601 gctccggaac tctggcgcga aacgcgcgcg caaatccatg tcgcgcgtgc tgcggggctc
     3661 gatgtcaacg gccaggtcgg ttgccggcca atcggcgtga tgatgggtct cgaaactacc
     3721 attcatccgt tttcctcgca cccggcctgg cttgaaatga gttcgctcaa tccggcgcag
     3781 cgcgtgacac gacttcgcca gagtgtggaa ctcaggcgcc agctggtgga ggaacgtccg
     3841 gcggacgaac acaccaaacg catcgccgat gcatttggtc gcatctatgt tgtcgacgat
     3901 aactacgatt acgaacccga tccatccctc agtatcggcg cacgcgcgaa gaccacgggt
     3961 actagcccat gggcgctggc gctcgatgca atgatggcgt gcgagggtaa acagttgctg
     4021 gttgaaacgt tcgagaacta taccgaaggc aacctcgata acatccgcga aatgctcgtg
     4081 gacgaggcca cagtcatggg catcgcggat ggcggcgccc acgtctgcac cgtttgcgat
     4141 gccagctctc ccacttttct gcttacgcat tgggcccgcg atcgtaaacg cggcgagcaa
     4201 ctaccgctgg aattcctgat ccgcaagcag acccgggatt gtgcgctggc ttacggactg
     4261 aaggatcgcg gcgtgctggc cgcgggctac cgggccgatc taaacatcat cgatttcgcg
     4321 agattgggca tgcgcaagca tgaagtcgtc tacgatctgc cggcaggcgg gcgcaggctg
     4381 atgcaacgcg cagacggtta ccgccatact tttgtcgccg gagtcgaaac gctgtgcgac
     4441 gatcagcaca ccggggccat gcccggaaaa ctggtgcgct gagcaggcca tgagcaagat
     4501 tttccgggtg gagtacccaa tccgcttttc gcattgcgat cccgccggac aagttttctt
     4561 tccgcgcttc ttcgatatgc tgcacgatgc gatggaagac tggttcaaca ccgggttgca
     4621 ggagcgcttt tccgatttgc tgatgaacca gaagctcggt accccaaccg tgggaacgca
     4681 gtgtgacttc atcagccccg cgcgctttgg cgacacgctg accatcgaac tgtcaatcct
     4741 gcggcttggc aacagttcca ttgaactggc gttcaatgcg agcatcgacg ggcgtgcctg
     4801 tctgaaatgc cgtcatacca tctgcatgtt ctccaaggat accgccaaag ccgtgccgat
     4861 tccggaagcg ctgcgcaatc gcatgcagga ttttgtcgtg ccggctaagt agcccgggaa
     4921 ctcgccgttc ggccttctgc ctaagctgca agattgacaa gagaggctgc catgagtcga
     4981 gatattctca aactgacttt gctgacttta ttcctggcgc tggtgctggc cggtgcgccg
     5041 atgtctttcg cggccgatgc cggcaaaacg accggtgcgg atgtcggaag aaagatggat
     5101 gagacggcgg agaccatcaa gaattattcc gtggaacagc gggacgaggc tttgaagaat
     5161 gccaaaaatc tgctggcgga agccgatggc cgtatcgagc aattggaaaa cgacatgagc
     5221 aagaactggg acgagatgaa cgcgtccgca cgcaaacatg cacgggacac catgcgcaca
     5281 ttgcggcggc aacgccagga cctgtcgcag agctatggcg agctaaagcg aagttcagcg
     5341 aatgcctggg atgaagtcaa aggcggtttc gcccgcagtt acgacgcgtt gcatgactcc
     5401 tttgcgaagg cggcgaagga attctagatc gagcatgagg cggtgcctta aagcccggtc
     5461 ctccgggctt ttttgttgcc tgtcgcttcc cgccacgcaa tctggttcgt ggtaatatac
     5521 gtgcatatgc acgtttatca ggaatgcttt gcatgaacaa catcgccatg gcgacccgca
     5581 agccagaact ctcttcaggc gcgggcagcg aatggtcagt agcttgcacc ggggccaggc
     5641 tgcgcaggtt gacccggcgc gtcaccgccc actatgaaca ccacttgcgg ccggccggcc
     5701 taaagctgtc ccaatattcc ttgctggtgc acttgtccga gaagccggaa tctttgagca
     5761 cgctggctga ccgcatggag atcgacagga cgacgttatc tcgaaacctg cagccgctgc
     5821 tttcccgagg tctggtcgac gagacctctg gcagcgatgg ccgtcaacga ttgtttgtgc
     5881 tgagtgctaa cggagctcgc ttgcgtgcgt ccgcgcgcaa gcagtggcgc caggcccaac
     5941 tcgcgttgga aaaacatctg ggcagtgatt ttgttgcaaa tctccatacc cagcttgaac
     6001 aggcattgtt gcgcttgaaa cccgcactcc cggaggagaa ctgaacatgg cgcgaagcag
     6061 cgaagcgcat tcctattggt tcatcgtcct gctggccgcg gcgctactga tggttaccat
     6121 gggctcccgg caatctcagg gattgttcct gtctccgctc aatacgtcca ccgggttggg
     6181 catcgcgacc atcagcctgg ccatggccat tgggcagttc gtttggggcg ccgtgcaacc
     6241 cattgcggga gcaatcgccg accgctatgg tcctggacga gtgcttgcgg gtggcatact
     6301 cctgctggcg ctgggttccg cgctcgtacc gttcatgacg agttcggcag gcctgatttt
     6361 ttcgctcggc gtcctggtgg cagcgggctc cggagcgggc agcttctccg tcctgatcgg
     6421 ctcagcgtcg cgtcatttgc ccgctgaaaa gcgcgggatg gcggctggta tcattaacgc
     6481 cggcggttcc ttcgggcagt tcgtattcgc gccgataacg caaaaactaa tcagtgcgct
     6541 gggctggatg ggtgccatgt ggtcgattgc cgcaatcgca ttggccgcgc ttccgatcac
     6601 gcgatcactg cgcggcaaac ccgccagcgc aacggaacaa gccagatcta tagcctctaa
     6661 tccttcggat aagggcatgc gcgttgcgct gcgcgaagcg ctgaaggacc gcagctatct
     6721 tcttctgcac gccggtttct tcacttgcgg attccatatt gccttcctgg tcacgcatct
     6781 gccgggagaa gtgcagttat gcggactgcc cgcacaagtg gccagctggt cgcttggcat
     6841 catcggcttg tcgaatatcg ctggcagcct gcttgccgga ttcgggaccc agcgctaccg
     6901 cagcaaatat attttgttct gga
//
LOCUS       DATMZQ010000315         3475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4056732, whole genome shotgun sequence.
ACCESSION   DATMZQ010000315 DATMZQ010000000
VERSION     DATMZQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4056732"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VN496_10135"
     CDS             complement(<1..946)
                     /locus_tag="VN496_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HXF17353.1"
                     /translation="MADGPILVTGAAGFIGFHVARRLLRDGHDVIGLDNMNTYYDPVL
                     KEARRAELAKSSRLRFVKLDLADRGGMTNLFKEFRFPYVIHLAAQAGVRHSLVDPFAY
                     VDANLSGFVTILEGCRHSGCRHLLYASSSSVYGSNTKLPFSVHDNVDHPLSLYAATKK
                     ANELMSHAYAHLFRIPMTGLRFFTVYGPWGRPDMAMWIFAKSIFAGEPIALFNNGNMR
                     RDFTYVDDVMESVVRLVKKPPAGDSKFSGKLPDPGSSSAPWRIYNIGNNKSVELLEVV
                     RLLEEAIGKKAKRELLPMQPGDVSATYADVDDLMREVDF"
     gene            1426..2709
                     /locus_tag="VN496_10140"
     CDS             1426..2709
                     /locus_tag="VN496_10140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXF17354.1"
                     /translation="MSRSGSGRQDVPALTGLRFVAAFSVLIGHGFSWILQNHETPGGV
                     VFWVSQIAGLGMTLFFVLSGFVIHYNYGNLVSKGLRGTAAFFWARFARLYPLFLLMML
                     VYVILSSRTLDLLNGHPERFGSILRALPYFVFSIHSWVYTLIDGNPLISAIGGGSPIT
                     WSISTEWFFYLAYPLIAWLILRARTPRLAIVLALLWCALWIAVTSGLYDRSAPIDAWA
                     VQYFGPDAGSEEHSQESFVRWLFYFSPYVRMGEFMLGAFMAQLYINIENRPVSHRENL
                     IGTVVFFAAAVSVIIITYLNYSPDVGTNIFRKMNMNFALAPTAALLVFCGARYKNAGS
                     RVLISRPILLLGAASYSIYLVHDVVLGIAVKLTGSAKHGFAYDLMKLVLLLTAVLLIS
                     LVLYAYYEAPARKWLRGLWGKRPAPAALAGASPAS"
     gene            3039..>3475
                     /locus_tag="VN496_10145"
     CDS             3039..>3475
                     /locus_tag="VN496_10145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF17355.1"
                     /translation="MGRLLEIVTPLHKATKRDYLARMVDDKVHCMMKAKEYEYDYWDG
                     DRRYGYGGYKFIEGRWSPVAKALIDIYGLKNGSRVLDVGCGKAFLLYEMKKILPDLQV
                     AGFDISRHGLSEARDNIKPYLFRYRAQDRYPFGDQHFDLVISLG"
BASE COUNT          723 a          972 c          937 g          843 t
ORIGIN      
        1 ggaagtcgac ctcgcgcatc aagtcatcga catcagcgta ggtcgcagaa acgtcgcctg
       61 gctgcatcgg tagcaactcg cgcttcgctt tttttccgat tgcttcctca aggagacgta
      121 caacttcgag gagctccaca gacttgttat tcccgatgtt atagatgcgc cacggcgcac
      181 tgctcgaacc ggggtctggc aattttcctg aaaattttga atctcctgcg ggtggttttt
      241 tgaccaggcg cacgactgac tccatcacat cgtcaacata cgtgaagtca cgccgcatgt
      301 taccgttatt gaacagcgca atgggttcgc ccgcgaaaat tgacttggcg aaaatccaca
      361 tcgccatgtc cgggcggccc cacggcccgt acaccgtgaa aaatcgcaat cccgtcatcg
      421 gaatcctgaa cagatgcgca taagcatgcg acatcagttc gtttgccttt ttcgtcgcag
      481 cgtaaagact taaaggatga tcgacgttgt cgtgtacgga gaacggcaat ttcgtattgg
      541 agccgtaaac tgatgaagat gatgcataca acaaatgtcg gcagccgcta tgacggcagc
      601 cctcgagaat ggttacaaat cccgaaaggt tggcatcgac atacgcgaaa gggtcgacca
      661 gcgagtgccg gacgccggcc tgcgctgcaa gatgtataac gtaaggaaag cggaactcct
      721 tgaacaagtt cgtcatcccg ccgcggtcag caaggtccaa tttcacgaac cgaagccgac
      781 tcgacttcgc aagctccgca cgccgcgcct ccttcagaac cgggtcatag taagtgttca
      841 tgttgtcgag gccgataaca tcgtggccgt cacgcaagag acgtcgcgca acgtgaaatc
      901 caataaaacc tgcagcgcct gtcaccagaa tcgggccatc agccatctac cgcattccgt
      961 ttctgtttaa ttcctgaccc gccatatgaa tttcttcaga ctacatcgcg cggtcgagtt
     1021 tgcctcgtgg ccaaaccgcg atcttcgctg aaaatcgacg tttgggatcc aacgcgtctg
     1081 tttagctgtt tcgtggacct gcccgcaata attctcgacc tcaatcttgc cgcctctgcc
     1141 gggacaggtc cggattgtac ttttggcctc ctgcagcgtt tgataacttg gcaataccgt
     1201 gacaatgtca gaaaatattg gtgcctaact tgagtatagc caatgcaacg attgagaaat
     1261 cgagccggcg ctgtaatccg aaggaccctc agatatgtgc cattctggct tcgctttcgg
     1321 cgagtccagc atggatacga gctccgcaac tggtatcgtc gcatcgcctt tgggcgggtc
     1381 ttttagtttg tgcgcgcact cgaattaaat gacgtttcgg ctagcgtgtc ccgcagcgga
     1441 tccggccgac aggacgtacc agcgctgacg ggcctgcgct tcgttgcagc cttctccgta
     1501 cttatcggac acggtttttc atggatcctg cagaaccatg agacaccggg cggcgtcgtt
     1561 ttttgggtca gccaaatcgc cggacttggc atgacacttt ttttcgtcct tagcggcttt
     1621 gtgatccatt acaactacgg aaacctggtg agcaagggtt tgcgcgggac agcggccttt
     1681 ttctgggcgc ggttcgcgcg cctctatccg ctttttctgc tgatgatgct ggtgtacgtg
     1741 atcctcagca gcaggactct cgatctgctg aatggtcacc cggagagatt cggcagcatt
     1801 ctgcgtgcgc ttccctattt cgtgttttcg atccacagct gggtttacac cctgattgac
     1861 ggaaatcccc tcatcagcgc catcggcggt ggctcgccga tcacgtggtc gatcagcaca
     1921 gagtggttct tttatctggc ctatccgctg attgcgtggc tgatcctgcg ggcccgcact
     1981 ccgagactcg caatcgtgct tgccctgctc tggtgcgctc tctggattgc tgtgacgagc
     2041 ggcctctatg accggtctgc tccaattgat gcgtgggccg tccagtattt cgggcccgat
     2101 gccggaagtg aggagcattc ccaggagtca ttcgtacgat ggctattcta cttttcgccc
     2161 tacgtgcgca tgggcgagtt catgcttggg gcattcatgg cccaacttta tatcaacatc
     2221 gagaaccgac cggtttccca ccgggagaat ttgatcggca cggtggtttt ttttgcagct
     2281 gcggtatctg tcattatcat cacttaccta aactacagcc cggatgtcgg cacgaatatt
     2341 ttccgcaaga tgaatatgaa tttcgctttg gcgcccactg cagcgttgct ggttttttgc
     2401 ggtgcccgct acaaaaatgc gggatcgcgg gtgctgatct cgcgccccat acttctcttg
     2461 ggtgcggcga gctattcgat atacctggtg cacgatgtcg tcctggggat cgccgtcaag
     2521 ttgaccggat cggccaagca cggcttcgcc tacgacctga tgaaactcgt tctgcttcta
     2581 acagcggttt tgctgatctc gcttgtcctc tacgcctatt acgaggcgcc ggcacgcaaa
     2641 tggctccgag gactttgggg gaagcgccca gcaccggcgg cgctagcggg ggcctcgccc
     2701 gcttcctgaa agtacgggcc gcggtttccg tgcgcgtcga aagcaattct ttcgagttcg
     2761 gtcgtcggca agtcgaaccg caccagcgcc cggtccctac gcttttgtgc attgttccgg
     2821 tcccgcgacc gcgcacctaa cccgatcgta cgatgccgcc catgtcaggc agcaacgcgc
     2881 cgacttatcg gcatgagcca gccatgaggt attcttgact ttcaaaagac tatcgggcac
     2941 gccccgcaag agatttcgat ctctggacac ttctgagatt ttgagccccc gcttttggcg
     3001 ctcgaatatc cggattgcta tggactgagg aaacaagaat gggccggctg ttagagattg
     3061 tgacgccgct tcataaggca acaaagcggg attatttggc gcggatggtg gacgacaagg
     3121 tccactgcat gatgaaagcc aaagaatacg aatacgatta ttgggacggc gaccggcgat
     3181 atggttacgg tggttacaaa ttcatcgagg gacgctggag cccggtggca aaggcgctga
     3241 tcgatatcta cggcctcaag aacgggtcga gggttctcga cgtgggttgc ggcaaggcct
     3301 tcctgctgta cgaaatgaaa aagatcctgc ccgacctgca ggtcgccggt tttgacattt
     3361 ccagacatgg tctttcggaa gcccgcgaca atatcaaacc ctatctgttc cgctatcgcg
     3421 cacaggaccg gtatcccttc ggcgatcaac atttcgatct ggtgatctcg ctcgg
//
LOCUS       DATMZQ010000316         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_606364, whole genome shotgun sequence.
ACCESSION   DATMZQ010000316 DATMZQ010000000
VERSION     DATMZQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606364"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VN496_10150"
     CDS             <1..412
                     /locus_tag="VN496_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXF17356.1"
                     /translation="EPKLFIGGEEALLALDLATRGWSMIYSDALTVHHYPSPNRDAVA
                     RRRLLTRNALWVAWLRRHPMTVLRHTLSALRNSARDPDALIGCIDALRALRWVIRHRR
                     MVPAHVEASWQLIERWLDQNAGSNAALPVSTGKR"
     gene            complement(428..>2817)
                     /locus_tag="VN496_10155"
     CDS             complement(428..>2817)
                     /locus_tag="VN496_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF17357.1"
                     /translation="TPRFHLMNSTGRLSRDIERGTASIGFLLSVGLFTIIPTLVEIIA
                     VMAILVYQYNDWFTAIIAGTFVLYTVFTVEFTQRREIYQRALNKLDSNANSHLVDSLI
                     NYETVKSYANEELEVRRFGGIMADWVQTGSRNQRALSILHIGQSSIIAVGVAAVMVLA
                     GREVLRGTMTVGDLVLVNAYVIQICLPLNTLGFVFRQVRDAIVNVESLFGLLRQQPEL
                     EDAPNARPLVVSQAQVCFNHVEFGYEPGQQILWDIDFVIPPGRTVAVVGGSGSGKSTL
                     ARLLMRLYEVRAGSIEVDGRDIRLITQDSLRSAIGIVAQDTSLFNDTIAYNIGYGRPE
                     AGMDEIVQAAKAAHIHDLICGLPKKYQTVVGERGLKLSGGEKQRIAIARAVLKNPPIM
                     VFDEATSALDAFAERAIQAELDRIARNRTTLVIAHRMSTVVGADTILVLEQGRIVERG
                     THEQLLARAGTYAEMWSLQQKQRELQQAQDRLAMQPVNLAHVVAAVIDALRPDIDSRT
                     ISLFTMIDSQSAQVTGDPAKLQQMVWDLCSNAIRTAAPGGRVEVLVQRVGPWVQLKIG
                     EAGGRAALSPQESEQSLDLLELRNLAQQFGGRLTLEPTASGTVYTVDLPPRALSVAER
                     PARSMSARLDFPAGAADLRDARIALVEDQDDARQLLASLLVTRGAVVQDNSTGMQAVD
                     WFNSHPHEQWPDLLICDIGLPDMDGYSVISRIRSIEAGQGIALVDRMPALALTGFAHP
                     QDRTRALLAGFQMHLGKPVDPIELLAVVAAMLGPHTGRRRDNAMPTGIAQHESKR"
BASE COUNT          548 a          853 c          837 g          579 t
ORIGIN      
        1 cgagccgaaa ttatttatcg gcggtgaaga agcattgctg gcgctcgatc ttgcgacccg
       61 tggctggtcg atgatctata gcgatgcgtt aaccgttcat cactacccat cgcccaatcg
      121 ggatgcggtc gcacgcaggc ggctgctgac gcgaaatgcg ctttgggtcg catggctgcg
      181 tcggcaccca atgaccgtgt tgcgtcacac gctaagcgca ttgcgcaaca gcgcgcggga
      241 tcctgatgcg cttatcggct gtatcgacgc cttgagagca ctgcgctggg tcatacggca
      301 ccgacgcatg gtaccggcgc atgtcgaggc gtcgtggcaa ttgatcgaac gttggttgga
      361 tcagaacgct ggcagcaatg cggccctgcc ggtctcgacg ggcaagcgat aaaccgcgtc
      421 gggcacttca gcgtttcgat tcatgctgcg cgatgccagt tggcattgca ttgtcccgcc
      481 gccggccggt atgcggcccg agcatggctg caacgacggc aagcaattcg atcgggtcca
      541 ccggtttgcc gagatgcatt tgaaaccccg cgagcagcgc tcgtgttcga tcctgcggat
      601 gcgcgaatcc ggtgagcgcg agagccggca tccggtccac cagggcgatg ccttgaccgg
      661 cttcaatgga tcttatcctg ctaatgacgc tgtagccgtc catgtccggc aaaccgatat
      721 cgcatatcag caagtcgggc cactgctcat gcgggtgcga attgaaccag tcgacggcct
      781 gcatgccggt cgaattgtcc tgaacgaccg cgccacgcgt caccagcaag gaggcaagca
      841 actggcgagc gtcgtcctgg tcctccacca atgcgatgcg tgcatcacgc aagtccgcgg
      901 cgccggcagg aaaatcaagg cgcgcggaca tggaccgggc cggtcgctca gccacggata
      961 gcgcgcgcgg cggcagatcg actgtataaa cggtcccgct cgcagtgggc tccaacgtca
     1021 ggcggccgcc aaattgctga gccaggttgc gcaattcaag caggtcgagc gactgctcgg
     1081 attcctgcgg cgacagcgcg gcccgtccgc ccgcttcgcc gattttcagt tgtacccacg
     1141 gcccaacgcg ctgcacgagt acttcgacgc ggcctcccgg cgccgcggtt ctgatcgcat
     1201 tgctgcacag atcccagacc atctgctgca gtttggccgg atctccggtt acctgcgccg
     1261 actgcgagtc gatcatggtg aacaggctga tggtgcggct gtcgatgtcg ggtctcaatg
     1321 catcgatcac tgcggcaacg acgtgcgcca gattgaccgg ctgcatggca agcctgtcct
     1381 gcgcctgctg caattcccgc tgcttctgct gcagcgacca catttccgcg taggtgcctg
     1441 ctctcgccag caactgctcg tgtgtgccgc gctccacaat gcgtccttgc tcgagcacaa
     1501 ggatcgtatc cgcgccaacc accgtcgaca tccgatgcgc gatgaccagg gtggtgcggt
     1561 ttcgcgctat ccggtccagt tcagcctgga tggcgcgctc ggcaaacgca tccagtgccg
     1621 acgtggcttc atcgaacacc atgatgggcg ggttcttgag cactgcacgc gcaatcgcaa
     1681 tacgctgttt ttcgccgccg gataatttca gcccgcgctc cccgaccacg gtttggtatt
     1741 ttttcggaag accgcaaatg agatcatgaa tgtgcgcggc cttggctgcc tgcacaattt
     1801 cgtccatacc ggcttcggga cggccatagc ctatgttgta tgcaatggta tcgttgaaaa
     1861 gactggtgtc ctgcgccacg atgccgattg cgctgcgcag gctgtcctgc gttatgaggc
     1921 gaatgtcccg gccatcgact tcgatgctgc ccgcgcgcac ctcataaagg cgcatgagca
     1981 gtcgcgcgag cgtcgatttt cccgagccgc tgccacccac caccgccacg gtgcggccgg
     2041 gcgggatgac aaaatcgata tcccagagta tttgctgccc gggttcatag ccaaattcaa
     2101 catgattgaa acaaacctgg gcctgtgaca cgactagtgg ccgcgcgttc ggggcgtctt
     2161 cgagttccgg ttgctgccga agcaaaccaa acaggctttc cacattgacg attgcgtcgc
     2221 gcacttgccg gaatacaaaa ccgagtgtgt tcaatggcag gcaaatctgt attacgtaag
     2281 cgttgacgag cacgagatcg cccacggtca tggtcccacg cagcacttcc cgccccgcca
     2341 gtaccatgac cgcggcgacg cctacggcaa tgatgctgct ttggccgatg tgcaatatcg
     2401 acaaggcccg ctgattgcgc gaaccggttt gcacccagtc ggccatgatg ccgccaaagc
     2461 gcctgacttc gagttcttcg ttcgcgtaag acttcacggt ttcatagttg atgagactgt
     2521 ccaccagatg gctgttggcg ttggagtcca gtttattcag cgcgcgttga tagatttccc
     2581 gtcgttgtgt gaattcgacc gtgaaaaccg tatacagcac gaacgtgccg gcgatgatgg
     2641 cggtaaacca gtcgttgtac tggtagacca ggatcgccat tacggcaatg atttcgacca
     2701 gcgtagggat aatcgtgaac aatccgacgc tcagcagaaa gccgatgctc gcagtgccgc
     2761 gctcaatgtc gcggctgagg cggccggtgc tgttcatcag atgaaagcgc ggggtga
//
LOCUS       DATMZQ010000317         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3024134, whole genome shotgun sequence.
ACCESSION   DATMZQ010000317 DATMZQ010000000
VERSION     DATMZQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3024134"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..938)
                     /locus_tag="VN496_10160"
     CDS             complement(87..938)
                     /locus_tag="VN496_10160"
                     /inference="COORDINATES: protein motif:HMM:NF025606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4344 domain-containing metallopeptidase"
                     /protein_id="HXF17358.1"
                     /translation="MNSVIAAAISVLILLISSVSLGRPALAAAPTVLKAGQIKIAYMP
                     PTNPEHQPIYELVQERRVLERMRDYLKPLRLPGRLLLKTEGCDGDANAWYEESDHSVT
                     ACYEYLADILTNAPQETTAAGVTREDAVVGPTIEVFLHEIGHAVFNMLKVPILGREED
                     AADHVASYLLLQLDKDTARRTVIGVAYMYGKELQAKTPGMKQFADVHGLPAQRFYNLL
                     CLAYGKDPELFADLVQGGYLPESRSDGCADEYRQVDYAMHKLITPYIDKKLKNDTVRK
                     KLLRPAN"
     gene            1225..2043
                     /locus_tag="VN496_10165"
     CDS             1225..2043
                     /locus_tag="VN496_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17359.1"
                     /translation="MHTYRIFIIVAGLMACAPLAQAEDEAAAGRNPALNDKWFFELGG
                     YFPTTGTTAQLDSSTAGVGTIVDFHNMLGMDERAQAANLAARWRFTERWRLEASYFET
                     RREGNRTTNQDIQWGDKFFPAGSQLNSSIDLNDARVSVGYSLYKTSDKELGIGFGVHV
                     MGYDARISNQVLGSESKRVTAPLPVITFYGGFSMDDKWSIAARLDRFSLTYNQYSGSI
                     TAMGLELLYQPFRHVGFGLGYQSLFIDFEATRGDFIGKINQTMQGPTAFLNASF"
     gene            complement(2096..>3127)
                     /locus_tag="VN496_10170"
     CDS             complement(2096..>3127)
                     /locus_tag="VN496_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXF17360.1"
                     /translation="DANSLAQVGRQENQAEFRAFRLVLLGRMGTDYQVSYLLAAEYNG
                     LDRDPNQNWELMDLAFTFPIGGPATKLTVGKTKETFAYEMVADSANLPQSERVLNPFF
                     VSRNIGAKITHIIGEDQRMTISAGAFNDWWAKSEDFGDAGTDVTGRLTGLVWDENQGT
                     RFLHLGLTGRYVGADNNTLRYRGRPESNVSSYYVDTGELPADHAWHVGLEALWNQGPY
                     SVLAEYNRAWVNSPSTGNPQFQGYYVTASWILTGETRPYDRTVGYARRVMPTQRWGAP
                     ELVARFSSEDLDSGSVSGGKFYKTYLGINWWATRRAKAGIGWGHTWLDRFGTTGNTDA
                     VLTRLQWIY"
BASE COUNT          642 a          907 c          880 g          698 t
ORIGIN      
        1 cctatgatcc ctggcgcctt tgtaatcatt ttctccacgg ggcgcggtta aatgcaacgg
       61 ccggtgcgca atacctcgcg agggacttaa ttagccggcc gcaagagctt tttgcgcacg
      121 gtatcgttct tcagcttttt atcgatatag ggcgtaatca gcttgtgcat ggcgtagtca
      181 acttgccggt actcgtctgc gcatccatct gagcgcgact ccggcagata acctccctgt
      241 accagatccg cgaataactc cgggtctttg ccataagcca ggcatagcag attgtagaag
      301 cgttgcgccg gcaagccgtg cacgtccgca aactgcttca tgcccggcgt cttcgcttgc
      361 aactctttgc catacatgta ggccacaccg attacggtgc ggcgtgccgt gtctttgtcg
      421 agctgaagca gcagatagga cgcgacatga tccgcagcat cttcttcacg cccgagaatc
      481 ggcaccttca gcatgttgaa aacggcgtgg ccgatttcgt gcaggaatac ttcgatggtc
      541 gggccgacca ccgcgtcctc tcgcgtaact ccggccgctg tcgtttcctg gggagcattg
      601 gtaaggatgt cggccagata ttcatagcaa gcggtcaccg aatgatccga ttcctcgtac
      661 catgcattcg cgtcgccgtc gcagccttcc gtcttcagca acagccgacc cggcaggcgc
      721 agcggcttga ggtagtcgcg catgcgttcc agcacgcggc gctcctgcac caattcgtaa
      781 atcggctggt gctccgggtt cgtcgggggc atgtaggcga tcttgatttg gccagctttc
      841 aggactgttg gtgctgctgc aagcgccgga cggcccagcg acacactact gatcaaaagg
      901 ataagcactg atatggcggc ggcaatgacg ctattcatcg tttgctcgcg ctcccgtggg
      961 gcaaaaaatg tcatcgaaag tgaccctgtg gtgggatatc agtcaatcac ctgtcttcaa
     1021 cctagtgtgc gtaattgctg agcttgacta aatagtttcg caatagtaaa cgaaactagt
     1081 aatactcggt ctgaaagccc gagtcgccgg accatgcgtg cggcgccgga actatttcaa
     1141 ccggccaagg gagtaaggta acctgcgccc gtcgactcga tgatgttgca tcttacagtc
     1201 cagtcatttc atgaggaaat caccatgcac acgtaccgga tattcatcat tgttgccggg
     1261 ctgatggcgt gcgcgccgct ggcccaggcc gaagacgagg cagcggctgg tcgcaatccc
     1321 gctctcaacg acaaatggtt ttttgagctc ggcggttact tcccaacaac gggcacaact
     1381 gcgcaactgg actcgtccac tgcgggcgta gggaccatcg tcgactttca caacatgctg
     1441 ggcatggatg aacgcgcgca ggcggcaaac ttggcagccc ggtggagatt caccgagcgc
     1501 tggcggctgg aggcatccta tttcgaaact cgccgcgagg gcaatcggac gactaatcag
     1561 gacattcaat ggggcgacaa attttttccg gccggctcgc agctgaactc cagtatcgac
     1621 ctcaatgatg cgcgggtttc agttggctac tcattgtaca aaaccagcga caaggaactt
     1681 ggcatcggtt tcggggtgca tgtcatggga tacgacgcga gaatttccaa ccaagtgctg
     1741 ggctcagaat ccaaaagagt cactgcgccg cttccggtaa ttacttttta tggtggattt
     1801 tcaatggacg acaagtggtc gatcgccgcg cgcctggacc gtttttcact tacctacaac
     1861 cagtacagcg gaagcatcac tgcgatgggc cttgagttgc tttaccagcc gttccgccat
     1921 gtcggattcg ggctcggcta tcaaagcctg ttcattgatt tcgaagcgac gcgcggtgat
     1981 tttatcggca agataaacca gactatgcag ggcccgacgg ctttcctcaa tgccagcttt
     2041 tgatgttcgc gaatgcgaac ttgccatgca gtcggcaggt gcgccgcacg tgtaattaat
     2101 agatccattg cagccgcgtc agcacggcgt ccgtgttgcc ggtcgtgccg aaacgatcga
     2161 gccaggtgtg gccccaacca attccggctt ttgcgcgccg cgtggcccac cagttgatgc
     2221 caagataagt cttgtagaat tttccaccgc tgaccgaccc actgtccaga tcctcgcttg
     2281 aaaagcgggc gacgagttcc ggcgcacccc agcgctgggt cggcatgacg cggcgtgcgt
     2341 agcccaccgt gcggtcgtag ggccgcgtct cgccggtaag gatccagctc gcggtgacat
     2401 agtagccctg gaattgcggg ttgccggtcg aaggtgaatt cacccaggcg cggttgtatt
     2461 cggcgagcac ggagtagggc ccttggttcc agagtgcttc cagtccaacg tgccacgcgt
     2521 ggtccgcggg cagctcgccc gtatcaacgt agtagctcga tacattggac tccggcctgc
     2581 ctcggtaacg cagcgtgttg ttgtcagccc cgacgtagcg gcccgtcagg ccgagatgca
     2641 ggaaacgcgt cccctgattc tcatcccaca ccagtccggt caagcgcccc gtgacatccg
     2701 taccggcgtc cccgaaatct tcgctttttg cccaccaatc attgaaggcg ccggcagaaa
     2761 tggtcatgcg ctgatcctcc ccaatgatat gcgttatttt tgctccgata ttgcgcgaga
     2821 cgaagaaagg attcagcacg cgttcggact gcggaaggtt cgccgaatcg gcgaccattt
     2881 cgtaggcgaa cgtttccttc gttttgccaa ccgtaagttt ggtcgccgga cccccgatcg
     2941 ggaaagtgaa tgccagatcc atgagttccc agttttgatt cgggtcgcga tccaggccgt
     3001 tgtattccgc ggccagcaga tagctgacct ggtaatccgt ccccatgcgc cccagcaaga
     3061 ccagacgaaa agcgcggaac tcggcctgat tctcctgccg tccgacttgc gccaggctat
     3121 tggcgtc
//
LOCUS       DATMZQ010000318         4451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1298337, whole genome shotgun sequence.
ACCESSION   DATMZQ010000318 DATMZQ010000000
VERSION     DATMZQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298337"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /gene="mraY"
                     /locus_tag="VN496_10175"
     CDS             complement(<1..801)
                     /gene="mraY"
                     /locus_tag="VN496_10175"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXF17361.1"
                     /translation="MLLALTQWLAEDAHIRAFNVFNYITLRAVLATLTALLISFIVGP
                     LMIRKLMAYKIGQSVRDDGPKSHLTKAGTPTMGGALILISIAVTALLWADLRNRYVWV
                     VLAVTLGFGIIGWIDDYRKVVHRNPKGISARTKFLWQSAIGLAAAIYLATSATLPAQT
                     ELIVPFFKQVAYPLGIVGFVVMSYFVIVGTSNAVNLTDGLDGLAILPTVMVGSALGVF
                     AYVAGNAIYSRYLGFPLIPGAGELTVVCAAIAGAGLAFLWFNAYPAEVF"
     gene            complement(801..2183)
                     /gene="murF"
                     /locus_tag="VN496_10180"
     CDS             complement(801..2183)
                     /gene="murF"
                     /locus_tag="VN496_10180"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXF17362.1"
                     /translation="MLDLLTAAQAVGAPLQGNNAKFERVTTDSRNVRAGDLFVGIRGE
                     KFDGRDFANQALADGAVGVMVQPGASAIASNASVIEVKDTRLALGILAAFWRARFSSP
                     LVAITGSNGKTTVKEMLAQMLRQVAGDSGVLATVGNLNNDIGMPLTLLRLRPEQRYAV
                     VEMGMNHLGEIAYLSSLAKPTIALINNAGTAHIGEVGSLDAIARAKGEIFDGLAKDGI
                     AILNGDDRFAGYWRSRLQGYRVMAFSLANRATVTARYELGLFASLVTFRTPEEEFVAS
                     LGVPGLHNIKNALAAVACAYALGIASTAMVAGLAQYSGVKGRLQRKRHASGAMVIDDT
                     YNANCESMQAAIAVLAACPGHKVFVMGDMGELGTEAEAMHASIGEFAQRSGIQELYAL
                     GTMSAAAVRRFGKGARHFSSIDELVGALSERIDQRSVVLVKGSRFMRMERVVESLGVI
                     NGNSPVEGTA"
     gene            complement(2176..3669)
                     /locus_tag="VN496_10185"
     CDS             complement(2176..3669)
                     /locus_tag="VN496_10185"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXF17363.1"
                     /translation="MNGRTRSVQHAAFSLCAIDALGVRFGGLATDSRKVQPNDLFLAY
                     PGSHNDGRAYIVQAIAAGASAVLWEYSGFQWNADWHVPNLGVENLRLHAGEIASRFFG
                     HPSRNMWLAGVTGTNGKTSCSHWLAQSLTHLGRKTAVIGTLGSGFLGELRDTVNTTPD
                     ALALQSTLAALRAGGATGCAMEVSSHGINQGRVNGTEFDVALFTNLSRDHLDYHKTMD
                     EYARAKAKLFDWPWLTHAVVNMDDDFGRELARSIDRSRISVLGYGFGRGEIAGHRLDL
                     STRGLNLEISTPWGPAQLKSRLIGEFNAANLLGVLGVLMTADVPLHDAIDALALVEPV
                     AGRMQMVRLPNAPLVVIDYAHSPDALEKALRALKMLLVPGAKLHCVFGCGGDRDPGKR
                     PLMGEIATRLADHVIVTSDNPRGEDPYTIIEDIVADAHPNFAIEVDRSSAIFQALRSA
                     VADDVVLIAGKGHETYQEIGMQRLPFNDVQVAREWLRRLIGTNAADA"
     gene            complement(3666..>4451)
                     /locus_tag="VN496_10190"
     CDS             complement(3666..>4451)
                     /locus_tag="VN496_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXF17364.1"
                     /translation="SMIDTGGGHYAIGSRTIHDAHPEGLLTVAQVIQKSSNVGSAKMA
                     LALTPQKLWAILSDVGFGTQTRVGFPGEASGRLRAYQTWKPIEQATMSYGHGISVSLL
                     QLARAYSVFATDGELKPLTLVRRDQPVKGKSVISARTALSVRKMLELVTQPGGTAVRA
                     QVAGFRVAGKTGTAHKLIGATYASDRYISSFVGFAPASHPRIVIAVMLDEPNGQSYYG
                     GEVAAPVFSTVMAGALRLLGIEPDAPLNNVILPDRKYVVREEV"
BASE COUNT         1042 a         1311 c         1160 g          938 t
ORIGIN      
        1 gaatacctcg gccggataag cattgaacca gaggaaggca agcccggcgc ctgcaatggc
       61 agcgcataca accgtaagtt ctcccgcacc cggaataagc gggaaaccca aatatcgcga
      121 ataaatggcg tttccagcga cgtaagcgaa aacaccaagc gcactgccga ccatgactgt
      181 tggcaggatg gcaagcccgt caagtccatc ggtcaaattg accgcgttgc tggtaccgac
      241 gatgacgaaa tagctcatga cgacgaagcc gacgattcca agcggatacg ccacttgctt
      301 gaagaatgga acgataagtt ccgtctgcgc cggaagcgtc gcggaagtgg caagatagat
      361 ggcggcggcc agtccgatgg cagactgcca gagaaatttt gtgcgcgccg aaatgccttt
      421 tggattgcgg tgcacgacct ttctgtagtc gtctatccag ccaatgattc cgaaacccaa
      481 cgtgacggcg agaacaaccc atacgtagcg gttgcgaagg tctgcccaca gtaaagcggt
      541 cacagcaatc gaaatcagaa tcagcgcacc acccatggtc ggcgtaccag cctttgtaag
      601 atggcttttt ggtccatcgt cgcgcactga ctggccgatt ttgtacgcca tcagcttgcg
      661 aatcatgagc ggtccgacga tgaaggaaat aagaagcgcg gtcagagtcg caagtaccgc
      721 gcgcaacgta atgtagttga atacgttgaa ggctcgaatg tgagcgtcct ctgcaagcca
      781 ttgcgtcagg gcaagcagca tcaggcagta ccctctacgg gcgagtttcc gttgatcact
      841 cccaacgact cgacaacgcg ttccatgcgc atgaaccgcg aacctttgac cagcacgaca
      901 gagcgttgat cgattcgctc ggacagtgcg ccaaccagtt catcaatact tgaaaaatgt
      961 cttgctccct tgccaaaacg gcgcactgcg gcggcactca ttgtccccag cgcatagagc
     1021 tcctgtatgc cggatcgttg ggcaaattcg ccaatcgatg catgcatggc ttcggcttcg
     1081 gtcccaagct cacccatgtc tcccatgacg aacactttgt gccccggaca agctgccaaa
     1141 acggcaatcg ctgcctgcat ggattcgcaa ttcgcgttgt atgtgtcgtc gattaccatt
     1201 gcgccggaag cgtgacgttt acgttgaagc cggcctttta cacctgaata ttgagcaagc
     1261 ccggcaacca tcgcagtcga agctattcca agcgcatagg cgcacgcgac cgctgccaac
     1321 gcattcttga tgttatgcag ccccgggact ccaaggctcg cgacaaattc ttcttcggga
     1381 gttcggaacg tcaccagact ggcaaacagg cctaattcat aacgcgccgt aacggtcgcc
     1441 cggtttgcga gactaaatgc catgacgcga tagccctgca gccgactgcg ccagtagcct
     1501 gcaaatcggt catcgccgtt tagtattgca atgccgtcct tggctagccc atcgaatatt
     1561 tcgccttttg cacgtgcgat agcatccagc gaaccaacct caccgatatg agcggtgcct
     1621 gcattgttga ttagagcgat ggtgggtttc gccaaagaag agaggtaagc aatttccccc
     1681 agatggttca tacccatttc gaccacggca tatcgttgtt cgggtctgag gcgcagcaag
     1741 gtgagtggca tgccaatgtc gttattaaga ttgcccactg tcgcgagaac tcccgaatca
     1801 ccagctacct gacgcagcat ctgggcgagc atctctttca ccgttgtttt gccattgctg
     1861 ccggtaattg cgacaagcgg cgatgaaaaa cgagcccgcc agaacgcggc taatatccca
     1921 agtgccagac gagtgtcttt cacttcgatg acggacgcat ttgacgcaat ggcactggcg
     1981 ccaggctgaa ccataacccc gactgcaccg tctgccagag cttggtttgc gaaatcccgt
     2041 ccatcgaatt tttcgccccg tatgccgacg aacagatcac ccgcccgcac gttacggcta
     2101 tccgtagtca ccctctcgaa cttggcattg ttgccctgga gaggtgcgcc aacggcctgc
     2161 gccgcagtca aaagatcaag catcggcagc gttcgtcccg atcaatcggc gcagccattc
     2221 tcttgccacc tgaacatcat tgaagggcaa tctctgcatg ccaatttcct gataagtctc
     2281 atgaccctta ccggcaataa gcacgacatc gtctgcaaca gcggagcgca gggcctggaa
     2341 tatcgcgctc gacctgtcca cttcgatagc gaaattaggg tgtgcgtccg caacgatatc
     2401 ttcgataatc gtgtacggat cttcgccccg cggattatca ctggtcacaa tgacgtgatc
     2461 tgccaaacgc gtcgcaatct cacccatcaa tggccgcttg cccggatcac ggtcgccacc
     2521 gcaaccaaat acgcaatgca atttcgcgcc tgggacaagc agcattttca atgcgcgtaa
     2581 cgccttttcc agtgcatccg gagaatgtgc ataatcaata accaccaaag gtgcgttcgg
     2641 cagcctgacc atttgcatgc gtccagcgac tggctctacc agcgccagtg cgtcgatagc
     2701 atcatggagc gggacatcgg ccgtcatcaa tacgccaagg accccaagca aattggccgc
     2761 attgaattct ccaatcaagc ggctttttaa ctgcgccgga ccccaagggg tcgaaatttc
     2821 aagatttagg cctcgtgtgg acaagtcaag ccggtgtccc gcaatctcgc ctctaccgaa
     2881 accgtatccg aggacgctaa tgcgggagcg gtcgatggat cgtgcgagtt cccgtccgaa
     2941 gtcatcgtcc atatttacta cggcgtgcgt cagccagggc caatcgaaaa gctttgcctt
     3001 cgccctagcg tactcgtcca tagttttgtg atagtcgaga tggtctcggg aaaggttggt
     3061 gaaaagtgca acgtcaaact ccgtaccatt gacacgacct tggttgatgc cgtgagaaga
     3121 gacctccatt gcacagcctg tggctccgcc ggcccgcaac gccgccagag tagattgcaa
     3181 cgccaaagcg tcgggtgtgg tattgacggt gtcgcgcaat tcgcccagga agccgctgcc
     3241 cagcgtcccg atcaccgccg tcttgcgccc caagtgagta agcgactgcg cgagccaatg
     3301 ggagcacgat gtttttccat tggtaccggt aacgcccgct agccacatgt tgcgcgaagg
     3361 gtgcccgaaa aagcggctgg cgatttcacc cgcgtgcaag cgcaaatttt cgacgcccag
     3421 gtttggcacg tgccagtccg cattccactg aaatccgctg tattcccaca gcactgcact
     3481 agcaccggcg gctattgctt gcacaatgta cgctcgtccg tcgttgtgcg accccggata
     3541 cgccagaaaa agatcgtttg gctggacttt gcggctgtct gttgcaaggc cgccaaagcg
     3601 cacacccaga gcatcaattg cacacaagct gaatgcggcg tgctgaacag agcgagtcct
     3661 tccgttcata cttcctctct aaccacatac ttgcggtcgg gaagtatgac gttgttaagc
     3721 ggagcgtctg gctcaatacc caatagccgc aaggcgcccg ccattacagt actgaaaaca
     3781 ggagcggcaa cttcgccgcc gtagtaagac tgtccattgg gctcatcaag catgacggcg
     3841 attactatcc gcggatggga cgcgggggca aacccaacaa acgacgagat atagcgatca
     3901 ctcgcgtacg tggctccaat aagtttgtgc gcagtcccgg tcttgcccgc aacacgaaac
     3961 cccgcgactt gagcccgtac ggccgttcct cctggctgcg ttaccaactc aagcatcttg
     4021 cgtactgaca atgccgtgcg cgccgagatc accgattttc ctttgacagg ctgatcacgt
     4081 cgtacgagcg tcaacggttt caattccccg tccgtggcaa ataccgaata agcgcgcgcc
     4141 agctgtaaca ggctgaccga aatcccgtgt ccgtatgaca tggttgcctg ctcgataggt
     4201 ttccaggtct ggtacgcacg cagccgtccc gatgcttctc cagggaagcc cactctggtt
     4261 tgggtaccaa aaccgacgtc ggaaagaatg gcccacagtt tctgtggcgt aagtgccagc
     4321 gccatcttcg cggagccgac gttcgaggat ttttgaatca cctgcgctac cgtgagcaaa
     4381 ccttccggat gcgcgtcgtg aatagtgcga ctaccaattg cataatggcc accacctgta
     4441 tcaatcatgc t
//
LOCUS       DATMZQ010000319         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4965964, whole genome shotgun sequence.
ACCESSION   DATMZQ010000319 DATMZQ010000000
VERSION     DATMZQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4965964"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VN496_10195"
     CDS             <1..218
                     /locus_tag="VN496_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXF17365.1"
                     /translation="VRVTPALSSGIAIAGAIVNPALGIAALIAQKALKDPFSKLASFD
                     YTISGTWGDPVVARVVRVIPQVEKEGR"
     gene            403..1200
                     /locus_tag="VN496_10200"
     CDS             403..1200
                     /locus_tag="VN496_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXF17366.1"
                     /translation="MAGVQMASGPNVAANLNEAGRLIEIAAKQGARLIALPEYFAIMG
                     MRPADKVKFREKDGDGPIQRFLADTARKLGIWLVGCSVPMAAPSEGKIYNSCQLYNDS
                     GKLVARYDKIHLFNLDLGNERFAEEGTLEAGNQVKVVDSPFGRIGLSVCYDLRFPELY
                     RAMGDVEIIIVPAAFTETTGRAHWEPLVRARAIENLAYVLAPAQGGYHLSGRETHGDS
                     MVVDPWGTVLDRLPRGSGVVVASVNPAYQASLRKSLPALTHRVIHKI"
     gene            1313..2773
                     /gene="tldD"
                     /locus_tag="VN496_10205"
     CDS             1313..2773
                     /gene="tldD"
                     /locus_tag="VN496_10205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HXF17367.1"
                     /translation="MPNDPSNSHELLFATADRTLLTPYGLDVAGLDRVFGQIMTHKVD
                     YADLYFQYSRSEGWSLEEGMVKSGSFNIDQGVGVRAVSGEKTAFAYSDDISLPALESA
                     AQATRAIASRGEQGATQVVSRTRGRELYLPQDPVASLADAEKVRLLEKLERYARAIDP
                     RVSQVMASLAGEYEVVLIARCDGMMAADVRPLVRVSVQVIVEQHGRREQGNAGGGGRF
                     DYAYFTDEVLSDYAAKAVHQAVTNLSARPAPAGTMTVVLGPGWPGILLHEAIGHGLEG
                     DFNRKGSSAFSGRIGQRVAAPGVTVVDDGTISKRRGSLNVDDEGNPTQRSVLIEDGIL
                     RGYIQDTLNARLMSMTPTGNGRRESYAHVPMPRMTNTYMLNGDKDPAEIIAGVKSGLY
                     AANFGGGQVDIVSGKFVFSAAEAYMIQDGEITHPVKGATLIGNGPDALTHVSMIGNDM
                     KLDSGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTA"
     gene            2819..3949
                     /locus_tag="VN496_10210"
     CDS             2819..3949
                     /locus_tag="VN496_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17368.1"
                     /translation="MILILSENTDPQTPEYLQLMESLGRLPNIQPRVHRIQGTERALT
                     EVYLLGDTKALSIEDMKSLPCVERVIRVSEEFRVLGRHKGDDRQTHFDYQGLRFGQDT
                     LHLFAGLCAVDTREHVDIMMRALKENGQQCTRMGAYKPRTSPYAFQGHGKNCLPYVFE
                     LAGKHGIRVIAMEVTHESHVAEIRQALHDTGHPTGVMLQIGTRNTQNFELLKIVGRQR
                     EFPVLIKRGFGITLDESLNAAEYLASEGNHRVIFGLRGMKTNLGDPHRNFVDFAHVPA
                     VKRLTRMPVCIDPSHSVGSRMAGPEGILDVMHATAQGVLAGANMVLVDFHPNPAKALV
                     DGPQALRLDELPHFIADVAIAREAYLRRAELAHRQMPAAMLS"
     gene            4086..4619
                     /locus_tag="VN496_10215"
     CDS             4086..4619
                     /locus_tag="VN496_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXF17369.1"
                     /translation="MANPDLQVPQNALLNTLEHLGEGTVLAQQIFDMIGHSRFFADFT
                     REDIQRLTGFMQVYRAEAAQMMIREGDIDDYMLLIIQGRVEIVKIDTHGHLQAMTTVG
                     SGATLGEMSMIDGEPRFATCMAIEPTTFAALSRDSMVQIILEEPGLGAKVLVKLVTLL
                     SQRLRQTSSSLLQYMER"
     gene            4625..>5360
                     /locus_tag="VN496_10220"
     CDS             4625..>5360
                     /locus_tag="VN496_10220"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXF17370.1"
                     /translation="MRCWRAALARACFFLIGAAMSAYLTGPAAALSVADDEGIELHLD
                     RPAQRVISLSPHLTEMMYAIGAGARLVGTVRGSDFPVEAASLPQIGDASGLDFERIVA
                     SRADLLLAWGSGNRSVDIARLRALGLKVLVLEPRRLEDIPRHLSVLGEVTGLRQQALA
                     PSRNFESRVQRLRQRYAGRVRVRVLFTIWYQPLFTINGDHIISKVLDLCAADNVFASL
                     PRLASEVSLEQVLLLDPDAIVVGSEAD"
BASE COUNT         1104 a         1551 c         1650 g         1055 t
ORIGIN      
        1 cggtgcgagt cactccggcg ctgtcgagcg gcattgccat tgcgggcgca atcgtcaacc
       61 cggcccttgg catcgctgca ctgattgcac agaaggccct gaaggatcca ttcagcaaat
      121 tggcctcgtt cgactatacg atcagcggaa cctggggcga cccggtcgtc gctcgcgtcg
      181 tgcgcgtcat tccgcaggtg gaaaaggaag gtcgctagag cttcggcgcc ggcgggaggt
      241 tacaatccat gctcgctaca ggtacacgca aggcttcgta actgcatggt cactaccacc
      301 ctgaaatccg gcttcgggcg cgcaaagcaa cgtagcgcgc gcgcccatgc cacgccagtc
      361 accgcggtcg tgaaaggaca ggccaaggca accgcattcc gcatggccgg tgtgcaaatg
      421 gcctcgggcc cgaacgtcgc ggccaatttg aacgaagccg gccggctcat cgagatagcc
      481 gcgaagcagg gggcgaggct gattgcgctg ccagaatatt ttgccatcat gggcatgcga
      541 cccgccgaca aggtcaaatt ccgggaaaaa gacggtgacg gtccgatcca gcgcttcctc
      601 gccgatacgg cgaggaaact gggcatctgg ctggtcggtt gttcggtacc gatggccgcg
      661 ccatctgagg gcaagattta taactcctgc cagctttaca acgacagcgg caaactggtt
      721 gcgcgttacg acaagattca tttgttcaac cttgatctcg gcaatgaacg cttcgccgaa
      781 gagggcacgc tggaggccgg taatcaggtc aaggtcgtcg attcaccgtt tgggcgcatc
      841 ggattgtcgg tttgctatga cttgcgtttc cccgaactgt accgggccat gggagatgtc
      901 gaaattatca tagtgccggc cgcctttacc gaaaccacgg gccgcgctca ctgggagcct
      961 ctggtgcgag cgcgcgcgat tgagaatctt gcgtacgtgc tggcaccagc gcagggcggg
     1021 tatcacttga gcgggcgcga aacgcatggc gattccatgg tggtggaccc gtggggcacc
     1081 gtgctcgata gattaccccg gggatccggc gtcgtcgtgg cttctgtcaa tccggcttat
     1141 caagcaagcc tgcgcaagag cctgcccgca ctgacgcaca gggtgatcca taaaatctga
     1201 aatgccgagg ttgaccctcg cgcacgactg gtaaaagaaa atttggtccc aacatacacc
     1261 gggcatggcc agcgcgccgt tacggtaatc agtacagaat acaaggagaa gcatgccaaa
     1321 cgatccctcc aatagccacg aactgctatt cgctaccgcg gatcgcacct tgttaacccc
     1381 ttacggtctc gatgtggcgg gtcttgatcg tgtattcgga caaatcatga cccacaaagt
     1441 cgattacgcg gatctgtact tccagtattc gcgttccgaa ggctggagcc tggaagaagg
     1501 catggtgaag tccggcagtt tcaacattga ccagggggtg ggcgtacgtg ccgtcagcgg
     1561 cgaaaaaacc gccttcgcgt actccgacga tatcagcctc ccagcgctgg aatctgccgc
     1621 acaggctacg cgtgcgatag cgagccgcgg cgagcagggt gccacgcagg tagtgagtcg
     1681 cacgcggggt cgcgagctct atctgcctca ggatccggta gccagcctgg ccgatgcaga
     1741 aaaagttcgc ctgctggaaa aactggaacg atacgcgcgt gctatcgacc cgcgggtttc
     1801 gcaagtcatg gcttcgctgg ccggggaata tgaggtcgtg ctgattgcgc gttgcgacgg
     1861 catgatggca gccgacgtgc ggccgctggt cagggtgtcg gtgcaagtca ttgtcgaaca
     1921 gcatggccgc cgcgaacagg gcaatgcggg tggcggcggg cgttttgatt acgcttactt
     1981 taccgatgaa gtgctgtccg actatgcggc gaaggccgtg catcaggcgg tgacaaatct
     2041 gtcggcgcgg cctgcgccgg ccggcaccat gacggtggtg ctggggcctg gctggcccgg
     2101 tatcctgttg cacgaggcca taggccacgg cctggaaggc gatttcaacc gtaaaggcag
     2161 ctccgcattt tccggccgca tcggacagcg cgtcgccgcg ccaggcgtga cggtggtcga
     2221 cgacggtact atttcgaaac gccgcggatc actgaacgtg gatgatgaag ggaatccgac
     2281 gcagcgcagc gttttgatcg aggacggcat cttgcgtggt tacatccagg acacgctgaa
     2341 cgcgcgcttg atgagcatga cacccaccgg caatgggcgg cgtgagtcgt acgcgcacgt
     2401 gccgatgcca cgcatgacca acacgtacat gctgaacggc gacaaggatc ccgccgaaat
     2461 tatcgctggc gtaaaaagcg gcctgtatgc cgcaaacttc ggcggcggac aggtcgatat
     2521 tgtcagcggc aaattcgtat tttcagcggc cgaggcttat atgatccagg atggcgagat
     2581 tacgcatccg gtgaagggcg ccaccctgat cggcaacggg cccgatgcgc tgacacatgt
     2641 gtccatgatt ggcaacgaca tgaagctcga ttcgggcgtt ggcacgtgtg gcaaggaggg
     2701 tcagagcgtg cccgttggcg taggccaacc gaccttgcgc atcgacggat tgacggttgg
     2761 tggcacagcc tgaaagccac ctgcccgata aagaaaacgc tggcatgacg acagccacat
     2821 gattctgatc ctttctgaaa atactgatcc gcaaaccccc gaatacctgc agttaatgga
     2881 atcgctgggg agattgccga acatccaacc gcgcgtgcac cgcatccagg gcacggagcg
     2941 cgcgttgacc gaggtctatc tactgggcga caccaaggcc ttatcgatcg aagacatgaa
     3001 gagcctgccg tgcgttgaac gggtgattcg tgtctccgaa gagtttcgcg tgctcgggcg
     3061 ccacaagggc gacgatcggc agacgcattt tgattatcaa ggcctgcgct tcggacagga
     3121 cacgctgcac ctgttcgcag gactgtgtgc agtggatacg cgcgagcatg tcgatatcat
     3181 gatgcgtgcg ctgaaggaaa atggccagca gtgcacccgc atgggggctt acaagccgcg
     3241 caccagtccg tatgctttcc agggccatgg aaaaaactgt ttgccgtacg tttttgaact
     3301 ggccggaaag cacggcatcc gcgtcatcgc catggaggtc acccatgaat cgcatgtcgc
     3361 ggaaattcgt caggcgctgc acgacacagg ccatccaacg ggcgtcatgc tgcagatcgg
     3421 cacgcgcaac acgcaaaatt tcgagttgct gaagatcgtc ggtcgccaga gggaattccc
     3481 cgtcttgatc aagcgtggtt tcggcatcac cctcgacgag tcattgaatg cagcagaata
     3541 tctcgcgagc gagggcaatc accgcgtcat ctttggtttg cgcggcatga agacaaatct
     3601 tggcgatccc catcgaaact tcgtcgattt cgcgcacgtt cccgcggtga agcgtttgac
     3661 ccgcatgccg gtttgcatcg atccaagcca ttcggtaggt tcgcgcatgg cgggccccga
     3721 aggtatcctt gatgtgatgc atgccacggc gcaaggtgta ctcgccggcg caaacatggt
     3781 gctggtcgac tttcacccca atcccgcgaa agcgctcgtc gatggcccgc aggcgttgcg
     3841 tctggacgaa ttgcctcact tcatcgccga cgttgccatt gcgcgcgaag cctacctcag
     3901 gcgcgccgag cttgcgcacc gccagatgcc agccgcgatg ctgagctgaa caacgttcgc
     3961 agaccgatgc tttaccggtc gcggccagtc cagcataatg ggttgtcatg tcacgtcaac
     4021 ctttggggct gccagagtag cgagtgcagc gcctgggtcc accaccagag accagcctca
     4081 tgcaaatggc caatcccgat ctgcaggtgc cgcagaacgc gctgttgaat acgcttgaac
     4141 atcttggcga gggaacagtc ctcgctcagc agatattcga catgatcggg cattccagat
     4201 tctttgccga tttcacgcga gaagacatac agcgcctgac cggtttcatg caggtctacc
     4261 gcgccgaagc ggcacagatg atgatccgtg aaggcgatat tgatgattac atgctcctga
     4321 tcatccaggg ccgggtggag atcgtgaaga tcgacaccca tggccatctg caagccatga
     4381 cgaccgtagg ttccggggca acgctcggtg agatgtcaat gatcgatggc gaaccgaggt
     4441 tcgcaacttg catggcgata gagccgacca cattcgcggc cctttcacgc gacagcatgg
     4501 tgcagatcat tcttgaagaa ccaggcctgg gggccaaggt gctggtcaag ctggtgacac
     4561 tgctgtcgca gcgcctgcgc cagacaagtt ccagtctgct tcagtacatg gagcgctaat
     4621 cgccttgcgg tgctggcgtg ccgccctggc gcgcgcgtgt tttttcctca tcggtgctgc
     4681 gatgtccgcg tacctcacag ggccggcagc ggccctcagt gtggccgacg acgaaggcat
     4741 cgaactgcac ctggaccggc ccgcacagcg cgtcatctcg ctgtcacccc acctgactga
     4801 aatgatgtac gccatcggcg ccggagcgcg cctcgtgggc acggtgcgcg gcagcgattt
     4861 tcctgttgaa gcggcgtcgc ttccacagat tggagacgcg tccgggctcg atttcgaacg
     4921 cattgtcgct tcacgcgcgg atctgctgtt ggcctgggga agcggtaacc gctccgtcga
     4981 tattgcgcga ctgcgtgcgc ttggcctgaa ggtgctggtg ctggagccga ggcgcctgga
     5041 agacataccc cggcaccttt ccgtgctcgg cgaagtcacc ggcctgcgcc agcaggcgct
     5101 agcgccgtcg cgcaacttcg aaagtcgggt tcagcgactt cgtcagcgct atgccggacg
     5161 tgtccgcgtg cgggtgctgt ttacgatctg gtaccagccg ctgtttacga tcaacggcga
     5221 tcacatcatc agcaaagtat tggacttgtg tgcggcggat aacgtcttcg ccagtctgcc
     5281 ccggctggcc agtgaagtat cccttgagca ggtattgctg ctggacccgg acgccattgt
     5341 cgtgggttcc gaggccgata
//
LOCUS       DATMZQ010000320        14915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2204259, whole genome shotgun sequence.
ACCESSION   DATMZQ010000320 DATMZQ010000000
VERSION     DATMZQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204259"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1387
                     /locus_tag="VN496_10225"
     CDS             113..1387
                     /locus_tag="VN496_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17371.1"
                     /translation="MAIRGSTFRQETARFSLLAMCGSCDGRFFAHAMVSVYRISSLML
                     AGLVGITAWGTAEAAASGTPLPPWLLYCDTEPCMSSPPLVVASCPASDPKCIPARNTK
                     VVAQVDGRPINSIMVTLKPGDTSVRVVSGNGIADAVSVTAFQAPYIILGAEREVLLTY
                     YDIVPVWGHTTQISFISTSFASAAIDTVFYRHASYDTGTAAADLHARARNVIDTQQAM
                     TGVTTGHATVFFMPSEMSTVPGGEGNFSSGNGIINVNYGNPPYIAASGGIMQTAIPRF
                     AHEYTHELFNQIATSYRGNHSCLNEGIADALPYVAGFLPEAEFGPVGLRGMDFDSSCA
                     ALNEMHDVGNCYFWHVKKAGLLTQAFLHGIFHPQRSYDFDSCAQNTLKAGNNMLVLFT
                     EAAGGANMVPILDSMHIPHAASYEAAKQALGY"
     gene            complement(1501..3126)
                     /gene="groL"
                     /locus_tag="VN496_10230"
     CDS             complement(1501..3126)
                     /gene="groL"
                     /locus_tag="VN496_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882457.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXF17372.1"
                     /translation="MAAKEVRFRESARARIVQGANILADAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELQDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIVQE
                     GMKYVAAGMNPMDLKRGIDKAVVTIVEELKKLSRNITTSKEIAQVGSVSANGDPSIGK
                     IIAEAMDKVGKEGVITVEDGKGLDNELETVEGMQFDRGYLSPYFITDPEKQQAILEDP
                     YILFHDKKISSIRELLPVLEQVAKASKPLLIVAEEVEGEALATLVVNSMRGILKTTAV
                     KAPGFGDRRKAMLEDMAILTNGTVISEELGLKLENVQLKDLGRARRIEVGKENTTIID
                     GAGEKSAIQGRIASIKKQIDEATSDYDKEKLQERVAKLSGGVALIRVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVLPGGGVAYLRARTALTSIKASNDDQAAGVRIVSRALEEP
                     LRMIVANAGMEPSVVLAKVLEGKGNFGYNAATDEYGDLVQSGVVDPTKVARSALQNAA
                     SVAALVLTTDAMVAEAPKDEKAKAPAAMPEMDY"
     gene            complement(3147..3440)
                     /locus_tag="VN496_10235"
     CDS             complement(3147..3440)
                     /locus_tag="VN496_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXF17373.1"
                     /translation="MKIRPLSDRILVKRLEEERTLASGIVIPDTAAQKPDQGEVLAVG
                     NGKILEDGKVRPLEVKQGDKVLFGKYAGQTVKVKGEELLVIREEDVMGVLEAA"
     gene            3659..4474
                     /locus_tag="VN496_10240"
     CDS             3659..4474
                     /locus_tag="VN496_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HXF17374.1"
                     /translation="MSPLLTLQVHETLRAAARAHANGVQCSLDLERSTTTVDLAVDHW
                     TWEGRQYPWLESCNDRTIYYWGGNGFEPVARFTTSLIKLVPTPWGPPTFEIDGIKMLP
                     TAHDSPFADAQRKVGLIQPRGKVILDTCGGLGYFAYWCLQGQATQVLSYEKNPDVLWL
                     RSLNPWSPKADERLTLMLGDIGKQIASLADNSVDAILHDPPRFGIAGELYSQSFYDQL
                     ARVIRRKGKLFHYTGTPNKLTSGRDVPGEVAKRLRRAGFVTKSHGDGVLAAKE"
     gene            4629..5885
                     /locus_tag="VN496_10245"
     CDS             4629..5885
                     /locus_tag="VN496_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17375.1"
                     /translation="MEQSRISRRRRSLLQGVGAATALGTLGFPAIVRGQSDVLRIGHL
                     TPRTGFLGPVGEYAVMGINLAVEEINAAGGVMGRKIELIAEDSVNPQTASTKAERLIE
                     RDKVAAIIGEISSASGLAIAQVAQRNRTLFFNTGCNSDDLRGKSCNRYMFHTEAANTM
                     YVNACGHALLREGMVQGKKWYSLTADYAFGHDLLKVAKRFMTANGGQFAADELVATDI
                     TDFSPYMLKIRNAKPDIVVSNLAGNQITVFMKQYIEFGLQYPVVGFAFDTALAWGAGK
                     GNVLGTWPLVWSHEVKTPAAKKFTEAFIKKYDRPPENQAWGDYLSAKHLVQAMNELKT
                     TDSTKIAEYLEKGAKFDVLKTREGYYRNWDHQLMQEMYTIRFKPAAEVKDRWDLFTVS
                     APVPEAKQSLEVIAPTREQNNCTMPA"
     gene            5936..6811
                     /locus_tag="VN496_10250"
     CDS             5936..6811
                     /locus_tag="VN496_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17376.1"
                     /translation="MMLIPIFEQILNGLLAGAYYLLIALGLSLIFSLGGLVNLAHGAF
                     YALGAYFAVKLAEHIGFAGAFVVSPVLVALLGLLVERFLFRRFYREDPALGLLLTFGL
                     AMVAEQTLRLVFGAAPLPFSIPNALKGQVLAGDFIYSRYRLAMLAAAVAAVAALWYLL
                     QRTPFGRVVRAGVQNPDMVAALGISLAPYMSGVVAISVALAALAGVLLAPITGVHPAM
                     GAEILTASFVVVVIGGLGSFWGVVYAALIVSVVRGLTAYFFPPAAESSMYLLMLLVLL
                     FRPRGLFGERIVRFE"
     gene            6808..9330
                     /locus_tag="VN496_10255"
     CDS             6808..9330
                     /locus_tag="VN496_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXF17377.1"
                     /translation="MNPLRSPLAVSALIIVVLPFALLAGGLTLTSATDVVIFAIACMA
                     LNVLVGHTGLVSFGHGAWFGLGAYAAALSQKYWFAASVLWPALFALVFLLATAAVVGF
                     LVLRRRGVYFSLLTLAFSALTYAIAFRWTAFTGGENGLGGVTRATWFGLDLEDRWIYY
                     GVVAFIGLAVVYLLGRFHRSPIGSVLLGIRENEQRAQFVGYATTRYKLIAYVVSAALT
                     GIAGVLFAFHHRFASADPMAVAFSGELLAMVIIGGMRSLLGPALGALFYVLFREYLSI
                     WTPNWLLFFGLLFVGFVVFSPTGLVGVWRRLTAPMRAQTIEAAAMATRNIEADAPIPQ
                     FLTSAVRRTGAALSVDGIAKAFGGIHAVQDVALVVNDRTLHALIGPNGAGKTTLFNLV
                     SGLFPPDRGSVQLCARDITGQAPHRTVAAGLARSFQITNLFPALSIEENLRLAVQAGD
                     ASRFNGWTDTASIASVNQTTRELISFLGLSGIERADAGFLSYGGQRLLDMGLALAARP
                     QLLLLDEPLAGLAAAERERVGLLIKRISREIPVLLVEHDIDRVLALADRITVMNEGRV
                     LIEGNSEEVRRSEVVQQVYIGSGVSALAARPRAQAAIGGAALLSLQHVDTFYGKSHIV
                     RDASLTVREGEIVALLGRNGAGKSTLLKTIIGIARSGGGSIALGDLDLSALPAAQNAR
                     LGIGYVPQGRGLFAGMSVRANLELGRLKRSRGTGALWTEARILEFFPRLKHRLDTPAD
                     FLSGGEQQMVAVARALSGHVRVLLLDEPFEGLAPAVVEELFEAFDRLRGEIAMIIVEH
                     NLDLVLALADRAYVLERGRVVHEGPAAALREDIELRRRVLWM"
     gene            complement(9391..10461)
                     /locus_tag="VN496_10260"
     CDS             complement(9391..10461)
                     /locus_tag="VN496_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF17378.1"
                     /translation="MIFRQLFDLQSYTYTYLLGDSASLQAVLIDPVFEQARRDAALLN
                     ELGLRLCYTLETHVHADHVTGAWLLKRQFDSQIALAQASGAVGADRYLAHGDTIEFGQ
                     RSLQVRATPGHTNGCLTYVLDNESMAFTGDCLLVRGSGRTDFQHGDPHTLYRSVHSQI
                     LTLPQDCLLYPAHDYRGLSVTSVAEEQRFNPRLGGQIAEADFAGYMQNMPLPHPKQID
                     VAVPANLLCGQRDDGIVSLTAPTWAALTYTFAGIWEIQPHALEESAGAVQIIDVREHA
                     EYDGPLGHIAGAKLIPLGNLSARVGELDAGRPIVAVCRSGARSAQATVILQQAGYKDV
                     ANLAGGMLRWRADGRPVQGGVS"
     gene            complement(10568..11908)
                     /locus_tag="VN496_10265"
     CDS             complement(10568..11908)
                     /locus_tag="VN496_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXF17379.1"
                     /translation="MNPEDSPRHGRLRLPDKLVSLSGRDVFVTFLPVLLVAVLAAWLA
                     VKFIRPAPPKTLVMLAGPKESSYYTIADRYAKILARSGVKLQLVETDGAKDNLRRLAD
                     PKAKADVGFVLGGLTEGVDIAGLTSLGSVFVQPVLIIYRGRKPVELLTELAGARIAIG
                     PEGSGTNTLAMKFLAANGMDKGSTRLVGLDGDDAVEALKQGKVDAAFLMGELVRGQVT
                     RDLLKLPGMRMMNFRQADGYVRRLKFLSRLVLPEGAFDLGLDLPPRDVELVGPTVELI
                     AHDDLHPALSDLLISAAQEIHRPAGMYRKAGEFPAPLERDFPISPDAQRFYRSGTPFL
                     YKQLPFWLASLVDRFVVVLVPLVVILIPAVRLVPTIYRWRMRSRIYRWYGALMAIERD
                     MFADPTPEGKEQIRKRLHNIQSSVNNLHPPLAFADQLYVLREHISWVQERLVGN"
     gene            complement(11941..12378)
                     /locus_tag="VN496_10270"
     CDS             complement(11941..12378)
                     /locus_tag="VN496_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HXF17380.1"
                     /translation="MASLAALLSGLIFGVGLIISGMTDPSKVRGFLDVVGAWDPSLAF
                     VMGGAIMIGIFAFGLASRRSRALLGGPLRMPATWPIDRQLLLGSLTFGVGWGLAGYCP
                     GPAVASLLTGGMKPLIFTAGMLVGMAIFEIHGMLARSRTGEQL"
     gene            complement(12394..12822)
                     /locus_tag="VN496_10275"
     CDS             complement(12394..12822)
                     /locus_tag="VN496_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HXF17381.1"
                     /translation="MIDWAHFTPWASSAGGMLIGVAAAMLLLLNGRIAGISGIVGGLL
                     QPAAGDLAWRLMFVLGLVSAPVLYGLVASPPTVRIDASGTTLLIAGLLVGAGTRYGSG
                     CTSGHGVCGLSQLSPRSLVATLAFMATGFMTVLVVRHLLA"
     gene            complement(12857..14098)
                     /locus_tag="VN496_10280"
     CDS             complement(12857..14098)
                     /locus_tag="VN496_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF17382.1"
                     /translation="MNLHKILAAAVATASVFAAAASFAADSIKVAVIDPLSGPFANVG
                     EAMVRYTQLAADAINARGGAAKFEIIALDSKSSPQEAQLALKSAIDQGVRYINQSNGS
                     NVAAALIDAVNKHNERNPDKTVMYLNTGAVDPALTNDKCSFWHFRFDADAEMKMAALT
                     DAIASNPRSKKVYLLNQDYAFGQAVARAAREMLPKKRADIEIVGDELHPLGKIKDFSP
                     YIAKIKATGADTIITGNWGNDMALLVKASREAGFSPDFYTYYAGGLGSPPALGDAGIG
                     HLKQVTQWHANIGTDKVEKHYRAYRDKYKDVKDDYYWTTQYLALEMLSKAVEQTKSTD
                     PVKVGKALEGMKFDGDTGEVMMRADNHQLIQPLFVSTYVKVDGKDIKNDVERTGNGFK
                     TDRRIEGKDTVMQTTCKMERP"
     gene            complement(14276..14695)
                     /locus_tag="VN496_10285"
     CDS             complement(14276..14695)
                     /locus_tag="VN496_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXF17383.1"
                     /translation="MIEHKIVEFRTKGRGMTEISAEVGRAVASSRIRQGVCTLFLQHT
                     SASLILCENADADVRRDLETVLARLAPDGDPAYVHDTEGPDDMAAHVRTVLTSNSLTV
                     PVAEGRLMLGTWQGIYLWEHRITPHTRSLVVTISGET"
BASE COUNT         2836 a         4417 c         4383 g         3279 t
ORIGIN      
        1 acaaagcact tttcacgacg caatttgccc acggaaaaat tttttgcaga cggtgttgca
       61 ggcatgatct gcaacgcctg ccgcttgcat gctggcatga caaggtacaa acatggcaat
      121 tcgcggatcc acattcaggc aagaaaccgc tcgtttctca ttgctggcca tgtgcggttc
      181 atgcgacggt agattctttg cgcacgcgat ggtgtccgtg tatcgcattt ccagtttgat
      241 gttggcgggg ctggtcggca taacggcctg gggcactgca gaagcggcgg ccagcggtac
      301 tcctttaccg ccgtggctcc tgtactgcga caccgagcct tgcatgtcga gtccgccgct
      361 ggtggtagcg tcttgcccgg cttccgatcc gaagtgcata ccagcccgca acacgaaggt
      421 tgtcgcgcaa gttgatggca gaccgatcaa cagcatcatg gtaaccctca agccgggcga
      481 taccagcgtc agggtggtct cgggaaacgg gattgccgat gccgtttctg ttacggcctt
      541 ccaggcgcct tacatcatcc tcggcgcgga acgtgaagtc ctcttaactt attacgacat
      601 cgttccggtg tggggtcaca caacacagat cagtttcatt agcacttcat ttgcatccgc
      661 cgcgatcgac accgtttttt atcgtcatgc cagctacgat accggcactg cggcggcgga
      721 tttgcacgcc agggcgcgga acgtcatcga cacgcagcag gccatgactg gtgtgacaac
      781 ggggcacgcg accgtctttt tcatgccgtc cgagatgtca actgtgcccg gtggagaggg
      841 aaatttctct tccggcaacg gcatcattaa cgtgaattat ggcaacccgc cgtacatcgc
      901 cgcgtcgggc ggcatcatgc aaaccgcgat accgcgcttt gcacacgaat atacccacga
      961 attattcaat caaatagcaa cttcgtaccg cggcaatcac agttgcctga acgaaggcat
     1021 cgccgatgcc ttgccttatg tcgcgggatt tcttcctgaa gccgagttcg ggccggttgg
     1081 tttgagagga atggatttcg attccagctg cgcggccctc aacgaaatgc acgacgtggg
     1141 gaattgctac ttctggcatg taaaaaaagc aggactgctg acacaagcat tcctgcacgg
     1201 cattttccac ccacagcgaa gctatgattt tgattcttgc gcgcagaata cgctgaaggc
     1261 aggtaacaac atgctggtgt tgttcacgga agccgccggc ggggcaaaca tggttccaat
     1321 cctggattcc atgcatattc cgcatgcggc atcgtatgag gcggccaagc aggcactggg
     1381 ctattagcgc tgttcatggc actgtggcat cggcgatcca cctcgttcgc ttttctcagc
     1441 tcaaagaaaa agaccccgct cgcgcggggt cgttctggga ctgccaattc aagatgagac
     1501 tcagtagtcc atttccggca tggcggccgg tgctttggct ttttcgtctt tgggtgcttc
     1561 ggccaccatc gcgtccgtgg tgagaaccag ggccgctacg gaagccgcgt tttgcaatgc
     1621 ggagcgggcg actttggtag gatcgaccac gcccgattgc accagatcac catactcgtc
     1681 cgtggccgcg ttgtagccaa agttaccctt gccttcgagt accttggcaa gcaccacgga
     1741 gggttccatg ccagcattgg ccacgatcat gcgcagcggt tcttccagcg cgcgggacac
     1801 gatgcgaact ccggccgcct ggtcatcgtt gctcgctttg atgctggtca atgcggtgcg
     1861 tgcgcgcagg tacgcaactc cgccaccggg tagaacgcct tcctcgactg ctgcgcgcgt
     1921 ggcatgcaat gcatcttcca cgcgggcctt cttttctttc atctcgactt ctgttgcagc
     1981 gccgacgcga atcagggcaa ccccgccgga cagcttggcg acacgttcct gcaatttttc
     2041 cttgtcgtaa tcgctggtcg cttcgtcgat ttgcttcttg atggaagcga tgcggccttg
     2101 tatcgcgctc ttttcgccag cgccgtcgat gatggtggtg ttttccttgc cgacttcaat
     2161 gcgcctcgca cggcccaagt ccttcaattg cacgttctcc agcttgaggc ccaactcttc
     2221 cgagatcacg gtgccattcg tcaggatcgc catatcttcc agcatggcct tgcggcgatc
     2281 gccgaagccg ggcgccttga ccgcagtggt cttcagaatg ccgcgcatgc tgttcaccac
     2341 cagcgtcgcg agcgcttcgc cctcgacttc ttcggcgacg atcagcagtg gtttgctcgc
     2401 tttggcaact tgttccagca ccggcagcag ttcgcggatg ctagaaattt ttttgtcatg
     2461 gaacaggatg tacggatctt ccagtatggc ctgctgcttc tcaggatcgg tgatgaagta
     2521 cggcgacagg tagccgcggt caaactgcat gccttcgact gtttcaagct cattgtcgag
     2581 gcccttgccg tcttccaccg taatcacgcc ttccttgccg accttgtcca tcgcttccgc
     2641 gattattttg ccgatggagg ggtcgccgtt ggcggagaca gaaccgactt gcgcgatttc
     2701 cttcgacgtt gtgatattcc tggacagttt cttcaattcc tccacgatgg tgaccaccgc
     2761 tttgtcgatg ccgcgcttca aatccattgg attcataccg gcggcaacgt atttcatgcc
     2821 ttcctgcacg attgattgcg ccaataccgt tgcggtcgtc gtgccgtcac cggctacgtc
     2881 ggaggtcttg gaagcaactt ctttcaccat ttgcgcgccc atgttttcga acttgtcctg
     2941 caattcaatt tcctttgcca cggacacgcc gtccttggtc acggtcgggg cgccgaacga
     3001 gcgctccagt actacgtttc ggcctttcgg cccgagggtc accttgaccg catcggccag
     3061 aatgttggcg ccctggacga tgcgggcacg cgccgattca cgaaaacgaa cttctttggc
     3121 tgccatgttt gcattctcct tttgcttcag gcggcttcga gtacgcccat gacatcctct
     3181 tcacggatca cgagcagttc ttcgcctttg actttgactg tttgcccggc gtacttgccg
     3241 aacaggactt tatcgccctg cttgacttca agcggacgaa ccttgccgtc ttccagaatt
     3301 ttgccgttgc cgaccgccag aacttcaccc tggtctggtt tctgcgccgc cgtgtcgggg
     3361 atgacgatgc ccgaagcgag cgtgcgttct tcttccagac gcttgaccag aatccggtcg
     3421 ctgagaggac gtattttcat tggtagtgct ccttcctggt tgcaatattt cattcaaatc
     3481 gatggccccg acagcggggt cgcgcgctag cgaaattatg gacagccgtg acctttttca
     3541 aggggagcgc cgtaacgctt gcgttagagc aaaaaatatt ttcctgtcag ggatttaatt
     3601 gcgcagtcca gcagcggttc gctatgcttg acccctccgg gcctcgccag tcattgttgt
     3661 gtccccgctc ttaaccctgc aggtccacga aactttgcgt gctgccgcgc gcgctcatgc
     3721 gaacggcgtc cagtgttcgc tcgacctgga acgttcgacg accaccgtcg accttgctgt
     3781 ggatcattgg acttgggaag ggcggcaata cccctggctt gagagctgca acgacagaac
     3841 catctattac tggggtggca acggctttga accggtggcc cgtttcacga cgtcgctgat
     3901 caagctggtg ccaacgccgt ggggaccgcc tacgttcgaa atcgacggca tcaagatgct
     3961 gccgacggca cacgactctc cctttgcgga cgctcaacgc aaagtcggcc tgatccagcc
     4021 gcgtggcaaa gtcatattgg atacctgcgg gggactgggc tacttcgcgt actggtgcct
     4081 gcaaggccag gccacgcagg tgctgtccta cgaaaaaaat ccggacgtgc tctggctgcg
     4141 cagcctcaat ccatggtcgc cgaaagcgga tgaacgattg acattgatgc tcggcgatat
     4201 cgggaaacag atcgccagcc tggccgataa ctctgtcgac gccatcctgc acgacccgcc
     4261 gcgcttcggc atcgccggcg aactttattc gcaaagtttc tacgatcagc ttgcccgcgt
     4321 gatcaggcgc aaaggcaaac tgttccacta caccgggacc ccgaacaaac tgaccagcgg
     4381 acgcgatgtg ccgggcgaag tcgcgaagcg tctgcggcgc gctggtttcg tcacgaagtc
     4441 gcacggcgac ggcgtgctgg cggcgaagga atgaactcct ggacgatcat gagccgccca
     4501 gcgcagtgac ccaggttgtt acgctggaca gggaagcaag ctttgaatta taagtaccat
     4561 cgaattcagg gcaaccggct catccgggcc gagcacggag tgtcccgccc atctatcgag
     4621 gaggagccat ggaacagtcg cgcatcagcc gtcgtcgtcg gagtctgctg cagggagtcg
     4681 gtgctgccac ggcgctcggt acattaggat tcccggcgat cgtacgcggc cagtccgatg
     4741 tactgcgcat tggccatctg acgccgcgca cgggattcct cgggccggtc ggcgagtatg
     4801 ccgtcatggg tatcaacctg gccgtggagg aaatcaatgc cgccggcggc gtgatgggcc
     4861 gcaagatcga gctcatcgcc gaggacagcg taaatccgca aacggcctcc accaaggcgg
     4921 aacgcctgat cgagcgcgac aaggtagcgg cgatcattgg tgagatcagc tcggcctctg
     4981 gcctggcaat cgcgcaggtt gctcagcgca accgcacact gtttttcaac accggatgca
     5041 actccgacga tctgcgcgga aaatcctgca accgctacat gtttcacacc gaagccgcca
     5101 acaccatgta cgtcaatgcc tgcgggcacg cgctgctgcg cgagggcatg gtgcagggca
     5161 agaagtggta cagcctcacg gccgattatg cgttcggcca tgatttgctc aaggtggcca
     5221 agcgcttcat gaccgccaac ggcggacaat tcgccgcaga tgaactcgtg gctaccgaca
     5281 tcactgattt ttcgccgtac atgctgaaaa tccgcaatgc caagccggat atcgtggtat
     5341 ccaatcttgc cggcaatcag atcacggtat tcatgaagca atacattgaa ttcggtttgc
     5401 agtatccagt ggtgggattt gccttcgata ccgcgctggc ctggggcgcg ggcaaaggca
     5461 acgtgctggg aacctggccg ctggtctgga gccacgaagt aaaaacgcct gcagcaaaga
     5521 aattcaccga ggcgttcatc aagaaatatg atcgtcctcc ggagaatcag gcatggggag
     5581 actatctgtc cgccaagcat ctggtgcagg caatgaacga gctgaagacc acggattcga
     5641 ccaaaatcgc ggagtatctg gagaaaggtg ccaagttcga cgtgctcaaa acgcgcgagg
     5701 gctactaccg caactgggat caccagctga tgcaggagat gtataccatc cgtttcaaac
     5761 cggctgctga agtcaaggac cgctgggatc ttttcacagt gtctgcgccg gtgcccgagg
     5821 cgaagcaaag tctggaagtc atcgcaccga cacgcgagca aaataattgc accatgccgg
     5881 cgtgatgtga cccccgtagc gcgcgactga ccggcaaccc agcgcagcgc ttcgcatgat
     5941 gctcatcccg attttcgagc agatcctgaa cgggctgctg gccggcgcct actatttgct
     6001 gatcgcgctc gggctgtcgc tcatattttc gctgggcggg ctggtcaatc tggcgcacgg
     6061 cgccttttat gcgctcggcg cttattttgc ggtgaagctg gcagagcaca tcgggttcgc
     6121 cggcgcgttc gtggtgtccc cggttctcgt ggcgctgctc ggattgctgg tcgagcgctt
     6181 cctgttccgg cgtttctatc gtgaggatcc ggcgcttggc ctgctgctta ccttcgggct
     6241 ggcgatggtt gccgagcaga cgttgcgtct ggtgttcggg gcagcaccac ttcctttttc
     6301 cattcccaat gcgctcaagg ggcaggttct tgccggagat ttcatctatt cgcgctatcg
     6361 gctggcaatg ctcgccgctg cagtcgcagc ggtcgctgcg ctctggtatt tgctccagcg
     6421 cacaccgttc ggccgtgtgg tgcgcgccgg cgtgcaaaat ccggacatgg ttgcggcgct
     6481 cggcatttcg ctcgcgccgt acatgtcggg tgtggttgcc atcagcgttg cactggcggc
     6541 gctggctggc gttctgctcg cgccgatcac cggcgtgcat cccgccatgg gggcggaaat
     6601 cctcacggcc tcatttgtcg tcgtcgtcat cggcggcctg ggctcgttct ggggcgttgt
     6661 ctatgccgcc ttgattgtca gcgtggtgcg cggattgacc gcgtatttct ttccgccggc
     6721 cgcggaatcg tcgatgtacc tgctgatgct gctggtgctg ctgttcagac cgcgcggcct
     6781 gtttggcgaa cgcatcgtgc gcttcgaatg aacccgttgc gctcgccgct ggcggtttcc
     6841 gcgctcatta tcgtggtgct gccatttgcg ctgctcgctg gcggactgac gctgaccagc
     6901 gcaaccgacg tcgtcatatt tgccattgcc tgcatggcac tcaatgtgct cgttggtcac
     6961 accggcctgg tgtcgttcgg gcacggtgca tggttcggcc tcggtgcgta tgcagccgcg
     7021 ctatcccaga aatactggtt cgcagcgtct gtgttgtggc cggcgctgtt cgcgctggtg
     7081 tttctgctcg ccacagcggc tgtggtcggc ttcctggtgt tgcgccggcg tggagtctat
     7141 ttttcattgc tgacgctggc cttttctgcg ctgacatatg ccatcgcatt tcgctggaca
     7201 gcgttcaccg gcggcgagaa tggactgggc ggggtaacgc gtgccacctg gtttggattg
     7261 gatctcgagg accgctggat ttactacggc gtggttgcct tcatcgggct cgcagtggtg
     7321 tacctgctcg ggcgtttcca ccgctcgccg atcggcagcg ttttactcgg catccgcgaa
     7381 aatgagcagc gcgcgcagtt tgtgggctat gcgactacgc gctacaagct gatcgcgtac
     7441 gtggtctcgg ccgcgttaac cggcattgcg ggcgtgctgt tcgctttcca ccaccgcttc
     7501 gcatctgcgg atcccatggc ggtggcgttc tcgggagagt tgctggcgat ggtcatcatc
     7561 ggcggcatgc gcagtctttt aggaccggcg ctcggcgcgt tgttctacgt gctgtttcgc
     7621 gaatatctgt cgatctggac gccgaactgg ctgttgttct tcggtctgct gttcgtgggc
     7681 ttcgttgttt tttcgccgac cggcctggtc ggcgtatggc gcaggctcac cgcgccgatg
     7741 cgtgcccaaa ccatcgaagc agcagcaatg gcgacccgca acatcgaagc cgatgctccc
     7801 ataccgcaat tcctgacctc tgccgtacgg cggacgggtg ctgcactttc agtggacggc
     7861 attgccaaag ccttcggcgg catccacgcc gtgcaggacg tcgcgctcgt ggtaaacgat
     7921 cgcacgctgc atgcgctgat cggcccgaat ggcgccggca agaccacgct gttcaatctg
     7981 gtttcggggt tattcccgcc ggatcgcggg tcggtacagt tgtgcgcgcg cgacatcacc
     8041 ggccaggcac cacatcgcac tgttgcagca ggcctggcga gatcgttcca gattaccaat
     8101 ctgtttccgg cactcagcat tgaagaaaat ctgcgccttg cggtccaggc gggcgatgcc
     8161 agccggttca acggctggac cgacaccgcc tcgattgcat cggtcaatca gacgacgcgt
     8221 gagctgatca gctttctggg tttgtcgggc atcgagcgcg cggacgcggg ttttttatcg
     8281 tacggaggcc agcgattgct cgacatgggg ctcgcgctgg cagcgcgtcc gcagcttctg
     8341 ctgcttgacg agccgcttgc cggactcgcc gcggccgagc gcgaacgcgt cgggctgctg
     8401 atcaagcgca tctcgcgcga aattccggtg ctgctggtcg agcacgacat cgatcgcgtg
     8461 ttggcgttgg cagaccgcat cacggtcatg aacgaaggac gcgtgctgat cgagggtaat
     8521 tctgaggaag tacggcgaag cgaagtcgtt cagcaggtgt atatcggctc aggagtgtcc
     8581 gcgttggcag cgcgtccgcg cgctcaggct gcgatcggcg gcgcggcgct gttgtcactg
     8641 cagcatgtcg atacgttcta cggaaagagc cacatcgtgc gcgatgcctc gctcacggtc
     8701 cgggagggcg agatcgttgc attgctcggc cgaaacggcg ccggcaaatc gaccttgctg
     8761 aagaccatta ttggtatcgc gcgcagtggc ggtggcagca tcgcgctcgg agatctggat
     8821 ctcagcgcgc tgccggctgc ccagaacgcc cggctgggga tcggctatgt gccccaggga
     8881 cgtggcctgt ttgccggcat gagcgtgcgc gcgaacctgg aattgggccg gctcaagcgc
     8941 agtcgcggaa ccggcgcgct ctggacagaa gcaagaattc tggaattttt tccgcgcctg
     9001 aaacatcgtc tcgacacgcc cgctgacttc ctgtccggcg gagagcagca gatggtggcc
     9061 gtagcgcgcg cactttcggg ccatgtacgc gtgctattgc tggacgagcc gtttgagggt
     9121 ctcgctcccg cggtggtcga ggagttgttc gaggctttcg accgtctgcg cggcgagatt
     9181 gcgatgatca tcgtcgagca caatctggac ctggtgctcg cgctggcaga tcgggcttat
     9241 gtgctggagc gcggcagggt ggtgcacgag ggcccggccg cggcgttgcg tgaagacatc
     9301 gaattacggc ggcgagtatt gtggatgtaa aaaggctggc tcgatgtcca cggtagccgc
     9361 ggtgtttgaa agacccgcag tcatgatttg ctaactcaca ccaccttgca ccggccgccc
     9421 atcggcgcgc cagcgcagca taccgccggc gagatttgcg acgtctttgt agccggcctg
     9481 ttgcagtatg accgttgctt gcgccgagcg agcgccggaa cggcagacgg cgacaatcgg
     9541 gcgaccggcg tccagttccc cgacccgggc ggacagattg cccagcggaa tgagctttgc
     9601 accggcgatg tgtcccagcg gtccgtcgta ttcagcatgt tcacgcacgt cgatgatctg
     9661 cacggcgcct gcgctttcct ccagcgcatg cggctggatt tcccagatcc ctgcgaacgt
     9721 gtaggtcaat gctgcccagg tcggcgcagt aagcgatacg atgccgtcat cccgctgacc
     9781 gcataacaga tttgccggca cggcgacatc gatctgcttt ggatgcggca agggcatgtt
     9841 ctgcatgtaa ccggcgaagt cggcttccgc gatttgtccg ccgaggcgcg gattgaagcg
     9901 ttgctcttcc gccacactgg ttaccgacag accgcgatag tcatgtgcgg gatagagcag
     9961 gcagtcctgg ggcaaggtca gaatttgcga gtgcaccgag cggtagagcg tgtgcggatc
    10021 accatgctga aaatcggtgc ggccgctgcc gcgaaccagc aggcagtcgc cggtaaacgc
    10081 catgctttca ttgtccagca cgtaggtcag gcatccgttg gtgtggcccg gcgtggcgcg
    10141 tacctgcaag gagcgttgac cgaattcgat cgtgtcgcca tgggcgagat agcgatccgc
    10201 gccgactgca ccacttgcct gcgccaaggc aatctggctg tcaaattgcc gcttcagcag
    10261 ccaggcgccg gtgacatgat ctgcgtgcac atgggtctcc agcgtataac acagccgcaa
    10321 tcccagttcg ttcaacagcg cggcgtcgcg ccgcgcctgc tcaaacaccg gatcgatcag
    10381 caccgcctgc aagctagcgc tgtccccaag caggtacgtg taggtgtacg actgaagatc
    10441 gaatagttgc ctgaagatca gcatgcgatg tccgccaatt ggctggctga aaatatacag
    10501 gcatcttaac ttgatattga gtgcgcggcg gttgctgagt attgggtcca gcggtgcgta
    10561 tcgctcacta gttccccacc agccgctcct gtacccagct gatgtgctcg cgcagtacat
    10621 aaagctggtc tgcaaacgca agcggcggat gcaggttgtt gacggacgat tgaatgttat
    10681 ggagccgctt gcggatctgc tctttgccct cgggtgtggg gtccgcgaac atgtcgcgct
    10741 cgatggccat cagtgctccg taccatctgt aaatgcgcga gcgcatgcgc cagcggtaga
    10801 tcgtcggcac caggcggact gcgggaatca gaatgaccac cagcggtacc agcacgacga
    10861 cgaagcggtc gaccaggctg gctaaccaga agggcaattg cttatagaga aagggcgttc
    10921 cggaccggta gaaacgttgc gcatccggac tgattggaaa atcacgctcc aggggagcag
    10981 ggaattctcc ggccttgcgg tacatgccgg cgggacgatg aatctcctgt gcggcggaga
    11041 tcagcagatc cgacaatgcg ggatgcaggt cgtcgtgtgc aattagttct actgtcggtc
    11101 cgaccagttc gacgtcgcgt ggcggcagat cgaggccaag atcgaaagcg ccttccggca
    11161 agacaagccg cgacaggaat ttcaggcgcc gcacgtaacc gtccgcctgc ctgaaattca
    11221 tcatccgcat gccgggcagt ttgagcagat cgcgagtgac ttggcctcgc accagttctc
    11281 ccatcaggaa agcagcgtcg accttgccct gtttgagcgc ttccactgca tcgtcgccat
    11341 cgagacccac taaccgggtg gaacccttgt ccatgccgtt ggcggccagg aatttcatgg
    11401 ccagggtatt tgtgccactg ccttccggac cgatggcgat gcgcgcgccc gccagttcgg
    11461 tcagcaactc aaccggtttc ctgccgcggt agattataag taccggctgg acaaacacac
    11521 tgcccagcga agtcagaccg gcgatatcga cgccctccgt cagaccaccc agcacgaatc
    11581 cgacatcagc tttcgccttg ggatcggcca gccgccgcaa attgtccttg gcgccgtctg
    11641 tctccaccag ttgcaacttc acgccgctgc gcgcaagaat tttcgcgtag cgatccgcaa
    11701 tcgtgtaata gctgctctcc ttcggcccgg ccagcatcac cagtgttttg ggtggcgccg
    11761 gacgaatgaa tttgacggca agccacgcgg caagcacagc caccagcagc accggcagga
    11821 acgttacgaa aacgtctcgc ccggacaagc tcaccaattt gtccggtagt cgcaaacggc
    11881 catggcgcgg tgagtcttcg ggattcatgt tttttatgcc gtcgcgcgca aaagtttggc
    11941 ttagagctgc tcgcctgtgc gtgacctggc gagcatgccg tggatctcga atatcgccat
    12001 tccgaccagc atgcccgccg tgaatatcag cggcttcatg ccaccggtca gtagcgaagc
    12061 tacggctggg ccgggacaat agccagccaa gccccaccca acaccaaacg tcaatgagcc
    12121 cagaagcaac tgtcggtcga taggccaggt cgcgggcatg cgcaggggcc cgcccagtaa
    12181 cgcccgcgat cgcctggagg caagtccaaa ggcaaatatg ccaatcatga tcgcaccgcc
    12241 catgacgaat gcgagcgacg gatcccaggc cccgacgaca tcaaggaaac cccgcacttt
    12301 ggaaggatcg gtcatgccag aaatgatcaa tcccactccg aatatcaaac cgcttagcag
    12361 tgccgccaaa gacgccagca tctgtatatc ctttcaagct aatagatggc gaacgaccaa
    12421 tacggtcatg aatcccgttg ccataaacgc cagagtggca accagagagc gcggcgacaa
    12481 ctgtgaaaga ccgcaaacac cgtgaccgct ggtgcatccc gagccatagc gggttcctgc
    12541 tccgaccagc aggccagcga tgagcagtgt tgtgccgctc gcatcaatgc gcacagttgg
    12601 gggagaggcg accaaaccgt agagcacagg ggcgctgacc aagcccaaaa caaacatcag
    12661 ccgccaggcc agatcaccgg ccgcgggctg cagcaggccg cctacgatac cgctgatgcc
    12721 cgcgatcctg ccgtttagca acagcagcat ggcggcagca acgccgatca gcatgccgcc
    12781 tgcgctggac gcccagggcg tgaaatgtgc ccagtcaatc atgaagccaa ggcacgtgct
    12841 ttcttccgac gagacactaa ggccgttcca ttttgcacgt ggtctgcatc accgtgtctt
    12901 tgccttcgat gcggcggtcg gtcttgaatc cgttgccggt gcgctccacg tcattcttga
    12961 tatccttgcc gtccaccttc acgtaggtcg aaacaaacag cggctggatc agctggtggt
    13021 tgtcggcgcg catcatgact tcgccggtgt cgccgtcgaa cttcatgcct tccagtgcct
    13081 tgccgacctt gacaggatcg gtcgacttgg tctgctcgac cgccttggac agcatttcca
    13141 gcgccaggta ttgcgtggtc cagtaataat cgtctttcac atccttgtat ttatcgcggt
    13201 aggcccgata gtgcttttcc actttatcgg ttccgatatt cgcgtgccac tgcgtgacct
    13261 gcttcagatg gccaataccg gcatcgccga gtgcgggcgg tgagcccaat ccaccggcgt
    13321 agtatgtata gaaatccgga gagaacccgg cttcgcggct cgctttcacc agcagtgcca
    13381 tgtcgttacc ccaattgccg gtaataatgg tatcggcgcc ggttgctttt atttttgcga
    13441 tatacggtga gaaatccttg atcttgccca gcggatgcaa ttcatcgccg acgatttcaa
    13501 tgtccgcgcg ttttttcggc agcatctcac gggctgccct tgcgaccgcc tggccaaacg
    13561 catagtcctg gttgagcaga tagactttct ttgaccgcgg gtttgaagca atcgcgtcgg
    13621 tcagcgccgc cattttcatt tcggcgtcgg catcaaaacg aaagtgccag aagctgcatt
    13681 tgtcgtttgt cagcgccggg tcgacggcac cggtgttgag gtacatcacg gtcttgtcgg
    13741 gattgcgttc gttgtgcttg ttgacggcat cgatcaacgc cgcggccaca ttggaaccat
    13801 tgctctggtt gatgtagcgc acaccctggt ctattgcgga cttcagcgcc agttgcgctt
    13861 cctgcgggct gcttttgctg tcgagcgcaa tgatctcaaa tttggcggcg ccaccgcgcg
    13921 cattgatggc atcggcagcg agctgtgtgt agcgcaccat tgcctcgccg acatttgcaa
    13981 aaggtccgga aagcggatcg atgacggcga ccttgatgct atccgcggcg aacgatgcag
    14041 cggccgcgaa tactgacgcc gttgccacgg cggcagctaa tatcttgtgc aaattcattt
    14101 gcgcctcctc gtcgttttat ttgatttatt ggctgtggac tgcgacaccc aggacaaacg
    14161 gaattttcgt cccatcggcg tccacagcgc aaactatgct gctcgtttgt ctgcggagtt
    14221 gcaaggatgc ggtccaccag ccctatatgc atggtgggcg gtattaaccg gttttttaag
    14281 tttcgccgct gatcgttacg acgaggctgc gcgtatgggg tgtgatgcgg tgctcccaaa
    14341 ggtagatccc ctgccaggtg cccagcatca gacgtccttc cgccacagga acggtcaaac
    14401 tattcgaggt cagaacagtg cgcacatgcg ccgccatatc gtccggaccc tccgtgtcgt
    14461 gcacataagc cgggtcaccg tccggcgcca accgcgctaa aacggtctcc agatcgcggc
    14521 gtacgtccgc atcggcattc tcgcagagga ttagcgacgc gctcgtatgc tggaggaaca
    14581 gcgtgcaaac accttggcga atgcggctgc ttgcgacggc gcgcccaact tccgcgctga
    14641 tctcggtcat gccgcggcct ttggtgcgaa attcgacgat tttgtgttcg atcataaaat
    14701 ggccgtgacg gcgtgaatgc cgtaaatgcc gtaaatgccg taaatgccgt aacgcctagc
    14761 tcttgaaacg cgcgatattc acgtattcac gatattcacg gcctttgccg tcaatgccgt
    14821 ggcccctgaa acgcccagcc ctcgaaacac gcgatattca cgtattcacg atattcacga
    14881 tatttacggc ctttcacaac tactggatgc atgac
//
LOCUS       DATMZQ010000321         5518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4062355, whole genome shotgun sequence.
ACCESSION   DATMZQ010000321 DATMZQ010000000
VERSION     DATMZQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4062355"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="fghA"
                     /locus_tag="VN496_10290"
     CDS             complement(<1..581)
                     /gene="fghA"
                     /locus_tag="VN496_10290"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HXF17384.1"
                     /translation="MKIISEHKCFGGIQGYYVHPSRETGLDMKISVFQPPQAQSHKVP
                     ALYYLAGLTCTEETFPIKSGAQRVASQLGLMLVSPDTSPRGANIPGETDAWDFGVGAG
                     FYVDATREPWAKHYRMYSYVTRELPALMASNFPADPVRQGIFGHSMGGHGALVCALRN
                     PGQYQSLSAFAPISAPMRCPWGKKAFTGYLGEER"
     gene            complement(625..1737)
                     /locus_tag="VN496_10295"
     CDS             complement(625..1737)
                     /locus_tag="VN496_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HXF17385.1"
                     /translation="MDVRAAVAHRAGAPLSLETVQLDGPKAGEALIEIKATGICHTDE
                     FTLSGADPEGLFPSILGHEGAGVVVDVGPNVKSLKKGDHVIPLYVPECRECEYCVSHK
                     TNLCQSIRVTQGQGLMPDGSSRFSLGGKKLHHYMGTSTFANYTVVPEISLAKIREDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGANVVIFGLGGIGLNVVQGARMAGANMIIGVD
                     INPKRRALAEKFGMTHFVNPKDMTEAELVPYLVNLTKGGADYSFECIGNVKLMRQALE
                     CCHKGWGVSVIIGVAPAGAEIATRPFQLVTGRVWKGTAFGGARGRTDVPRIVDWYMEK
                     KINIDDLITHKLAFKDINQGFDLMHAGESIRSVVVF"
     gene            complement(1844..2755)
                     /locus_tag="VN496_10300"
     CDS             complement(1844..2755)
                     /locus_tag="VN496_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXF17386.1"
                     /translation="MKENAIWMEDAVINEQDVAFYRKNGYLLVENVIDGPTLTKIRKA
                     IADLVAKAAGVKTHNDIYDLEPTHSPENPRVRRIKTPHKVDPFFFELVKQPGMISILK
                     ALLGQNVRLHGSKLNVKAPKYGSPVEWHQDWAFYPHTNDDILAIGVMLDDVELENGPL
                     LVMPGTHRINKVWNHHLDGRFCGAMDPTKTPDLDYSKAVPCIGKAGTCSFHHVRLVHG
                     SAQNTSEKPRQLLLYECTAADAWPLVNFKDLDEFNSRMLCGEPTLEPRTEHVPIRMPL
                     PPALAQGSIYENQTALRNRFFEVKAAT"
     gene            2983..4077
                     /locus_tag="VN496_10305"
     CDS             2983..4077
                     /locus_tag="VN496_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXF17387.1"
                     /translation="MNKLTAIAAAVGALCFAGTVSAQALKGPMDDSALSWETPEWGKE
                     DRAGSANHTKNSANIKRALATVKQFKAITIGKYYHREAPAFGARGWQMSIPGTPTGGP
                     FGKNALVYHDELVTTEIGQIQTQFDGPGHIGVNTSKGPMFYNGRISWDSYERGAGGRV
                     MGMGPLGVEHVGELGFVCRLVVLDAVAYKKSKGAIPASAEMLPIPKKAGDPGIVTADD
                     MQGMMKAQGLSDIQPGDCVALHTGQGNTWGNAHYKTMTSEQRAAARAIFAQGEPGWGI
                     SACKWMASKHIALQMGDTSANDAQPGGEEDGMAVPCHTEMMPKRGIWNLENVDTQSLV
                     AAGIKEGAFIWAPLRIIGATGSPGNPVVLY"
     gene            4184..4720
                     /locus_tag="VN496_10310"
     CDS             4184..4720
                     /locus_tag="VN496_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17388.1"
                     /translation="MRPIGNTAALAFVAAALWTAAPAAFACGEVFRGDMVTAEREVGA
                     TPSGEAATFHVLGAGWFHAAEIVKHGGSSDNTTVTLELDGEPIITASFADLKNGWREI
                     NTPMIVVNVRSTGDISTMTIWYAPELKFRALAALRIEVAEEGVNSLKMRTVMNKPAPH
                     EHVAGQSIGNTAALPAFK"
     gene            complement(4745..5389)
                     /locus_tag="VN496_10315"
     CDS             complement(4745..5389)
                     /locus_tag="VN496_10315"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HXF17389.1"
                     /translation="MRTCFCSLLAGTLLATVVLAEEPRQVMWADLVPASGAFDNPFLK
                     LTPEQLARVSEVAAIRDRRARGDKNLSQTEIDGEQKLSRKLVEAGVDVDGLLAKRKEI
                     IERGRSINPLLNGQVVRLPGYLLPLEFSGKMVSEFLLVPWVGACIHTPPPPPNQIVHV
                     TPERPVAMTGVFEPVWVTGPLRAAPLKKSLSLVDGSADIEVGYSIHASQVEPYK"
BASE COUNT         1162 a         1616 c         1609 g         1131 t
ORIGIN      
        1 cgttcttcgc cgagataacc ggtgaacgcc ttttttcccc atgggcaccg catgggcgcc
       61 gagatggggg caaaagcgga cagagactga tactgcccgg gattgcgcag cgcgcacacc
      121 agtgcgccat gcccacccat cgaatggccg aatattccct ggcgcacggg atccgccgga
      181 aagttgctcg ccatcagtgc tggcagttcg cgcgtgacgt aactgtacat gcggtagtgt
      241 ttcgcccagg gctctcgcgt ggcatcgaca tagaatccgg cgccgacacc gaaatcccag
      301 gcgtcggttt ctcccggaat attggcgcca cgtggactgg tgtcgggaga caccagcatc
      361 aggccgagtt gcgaggcgac gcgctgcgcg cccgatttga tcgggaaggt ttcctcggtg
      421 caagtcaagc cggccaggta gtacagcgcg ggtaccttgt gcgactgtgc ctgcggaggc
      481 tggaataccg aaatcttcat gtccagcccg gtttcccgcg acggatggac gtaataaccc
      541 tgtatgccgc cgaaacactt atgctcgctg attattttca tttcaatctc aaatactcag
      601 cgcgtgtggt gctccgtagg cgcatcaaaa cacgaccacc gagcggatgg actcgccggc
      661 atgcatcaag tcgaaaccct ggttgatgtc tttgaacgcg agcttgtggg tgatcagatc
      721 atcgatgttg atctttttct ccatgtacca gtcgacgatt cttggcacgt cggtgcgccc
      781 gcgcgcgccg ccgaacgccg tgcctttcca gacccgcccg gtgacgagct gaaacggccg
      841 cgtggcaatt tcggccccag ccggggcgac gccgatgatg accgacacac cccatccttt
      901 gtggcaacac tccaatgcct gccgcatcag cttgacgtta ccgatgcatt caaaactgta
      961 gtctgcgccg cctttggtca gattcacgag gtacggcacc agctcggctt cagtcatgtc
     1021 tttcggattc acgaagtgcg tcatgccgaa tttctcggcc agcgcacgac gtttgggatt
     1081 gatgtcgact ccgatgatca tgttcgcgcc agccatgcgc gcaccttgca cgacattcag
     1141 tccaatgccg cccagcccga atatgacgac gttggcacct ggctcgactt tagccgtgtt
     1201 gatgaccgca ccgatgccgg tggtgacgcc gcagccgata tagcagacct tgtcgaacgg
     1261 cgcgtcctcg cgaattttcg ccaaagagat ttccggcacg acggtgtagt ttgcgaatgt
     1321 tgaagtaccc atgtaatggt gcaatttttt cccgccaagc gaaaagcggc tgctgccatc
     1381 gggcatcagg ccttggccct gggtcacgcg aatcgactgg cacaagttag tcttatgcga
     1441 aacgcagtat tcgcactcgc ggcactccgg cacatacagc ggtatgacgt gatcgccttt
     1501 cttcaagctc ttgacgttgg ggccgacatc cacaacgacc cccgcgccct catgacccag
     1561 gattgacgga aacaaacctt ctgggtcggc tccggagagc gtgaactcgt ccgtgtgaca
     1621 aatgccggtt gcttttattt cgatcagggc ctcaccggct ttcggcccgt ccagctgcac
     1681 cgtctccagt gagagcggcg cgccggcccg atgtgcaact gctgcgcgaa catccatgct
     1741 atggctcctc tttttgttgt ctttttcgtg cgtccgatgc agcctcgcag gccccaaaaa
     1801 acaaacggcg ggactggccc gccgtcgtgg gatgctccgc gtatcaggtt gcggccttta
     1861 cctcgaagaa acggttgcgc aatgccgtct ggttttcata gatcgagcct tgcgccagcg
     1921 ccggcggcag cggcatgcgg attggcacgt gctcggttcg cggctccaag gtcggttctc
     1981 cgcacagcat gcggctgttg aattcatcga gatccttgaa attgaccagc ggccaggcat
     2041 cggccgccgt gcattcataa agcagcagtt ggcgtggttt ctccgaagta ttttgcgccg
     2101 aaccatgcac cagccgcaca tgatggaaag aacaggtccc ggctttgccg atgcagggta
     2161 ccgctttgga ataatccaga tcaggcgttt tggtgggatc catcgcaccg caaaagcgac
     2221 catcgaggtg atggttccaa accttgttga tgcgatgcgt gccgggcata accagcagcg
     2281 gaccgttctc cagctcgacg tcgtccagca tgacgccaat cgccaggata tcgtcgttgg
     2341 tatgcggata gaacgcccag tcctgatgcc actcgaccgg tgagccgtac tttggcgcct
     2401 tcacattgag cttggagccg tgcagccgca cgttctgtcc cagcaaggcc ttgaggatgg
     2461 aaatcatccc gggttgcttc accaactcaa aaaagaaagg gtccaccttg tggggcgttt
     2521 tgatgcgacg cacacggggg ttctccgggc tgtgcgtggg ctccagatca tagatatcgt
     2581 tatgggtctt tacgccggcc gccttggcaa cgagatccgc aatggccttc cggattttgg
     2641 tcaaggtggg gccatcgata acattctcga ccagcaggta gccgtttttg cggtagaagg
     2701 cgacgtcttg ttcgttgatc actgcatcct ccatccagat tgcattttcc ttcatcttac
     2761 gcctcccgca aagctcggga aacggttgtg cacattgccc aacctgccgg acggcgcatc
     2821 gcacaacgcc acgaccggga aatccggcta tttcaagttg aaatgtaaga aggagtatgt
     2881 cagtgtggct gcgttgcgcg gcggcatggg ctgttctgca tgccgaagga caacaggctt
     2941 tcaaagcaaa aaaacagtcg ataaccagga ggagaaatcg caatgaacaa acttaccgcc
     3001 attgccgcgg cagtcggagc cttgtgcttc gctggtacgg tatccgccca ggcactgaag
     3061 gggccgatgg acgacagtgc cctgagctgg gaaacgcccg agtggggcaa agaagacaga
     3121 gcgggcagcg cgaaccacac caagaattca gccaacatca agcgcgcgct tgccactgtc
     3181 aagcagttca aggccatcac catcggcaag tactaccatc gtgaagctcc ggcgttcggt
     3241 gcgcgcggct ggcaaatgag catccccggc acgcccaccg gcggcccctt cggcaagaac
     3301 gcgctggtgt atcacgacga gctggtcacc accgaaattg gccagatcca gacgcaattc
     3361 gatggtcccg gtcacatcgg tgtaaacacc tccaagggac caatgttcta taacggccgc
     3421 atttcgtggg attcctacga gcgcggtgca ggtggtcgcg tgatgggtat ggggccgctt
     3481 ggtgtggaac atgtcggcga actgggcttc gtctgccgac tggtcgtgct ggatgcggtg
     3541 gcgtacaaga aatccaaagg cgcgataccg gccagtgcgg aaatgctgcc gatcccgaag
     3601 aaggctggcg atcccggcat cgttactgcg gatgacatgc agggcatgat gaaggcgcaa
     3661 gggcttagcg acatccagcc aggggattgc gtcgcactgc ataccggcca aggcaatacc
     3721 tggggcaacg cccattacaa gaccatgacc tcggagcaac gagctgcagc gcgcgccatt
     3781 tttgcgcagg gcgaaccggg gtggggcatc agcgcctgca agtggatggc gtcgaagcac
     3841 attgctctac agatgggcga tacctcggcg aatgacgcac agccgggcgg tgaagaggac
     3901 ggcatggcgg ttccctgcca tacggaaatg atgcccaagc gcggtatctg gaatctggaa
     3961 aacgtcgata cccaatcgct ggtagcagcc gggatcaagg aaggcgcatt tatctgggca
     4021 ccactacgca tcattggcgc gaccggttcg ccgggcaacc cggtggtgct gtactaaaga
     4081 gctagcgaaa cagttctacc cctctaagtt aaagggcggt cacgcaagtg actgcccttt
     4141 ttttcatact tcaatacagg gcttcaagtg ctaaactgga acagtgagac ccataggtaa
     4201 cactgccgca cttgcctttg tcgccgccgc actatggacc gctgccccgg cggcgtttgc
     4261 gtgcggggaa gtgtttcgcg gcgacatggt gaccgcggag cgtgaagttg gcgccacgcc
     4321 ctccggagaa gcagctacgt ttcacgtact gggagccggc tggttccacg cggccgaaat
     4381 cgtgaagcac ggcggcagca gcgacaatac cactgtcacc ttggaactcg acggtgagcc
     4441 aattattacc gcgtcgttcg ctgacctgaa gaatggctgg agggaaatta atacgccaat
     4501 gattgtcgtg aacgtacgca gtaccggtga tatcagcaca atgaccatct ggtatgcacc
     4561 ggaactgaag ttccgcgcgt tggcggcgtt gcgcatcgaa gtagcagaag agggcgtcaa
     4621 cagcctgaaa atgcgcaccg tgatgaacaa gcccgcgccg catgagcacg tcgccggcca
     4681 gtcgatcggc aataccgccg ctctgccggc tttcaagtaa gtaacgccaa cggccgggcg
     4741 cgttttattt gtacggttcg acctggctcg cgtggatcga ataccccact tcgatgtctg
     4801 ccgatccatc cactagcgac agacttttct taagcggcgc cgccctgagt ggtccggtaa
     4861 cccatactgg ctcgaatacg ccggtcatgg cgacaggccg ttccggtgtt acatgcacga
     4921 tttgattggg tggtggcggc ggagtgtgga tacaggcgcc cacccacggt accagcagga
     4981 attcgctgac catcttgcct gagaactcca gcggtaacag ataacccggc aagcgcacaa
     5041 cctgtccatt gagcagcgga ttgatcgagc gcccgcgctc gatgatttcc ttgcgctttg
     5101 ccagcaagcc atccacatcg acgccggcct cgacgagctt gcgcgaaagc ttttgttcgc
     5161 cgtcgatttc agtctgtgac aaatttttgt cgccgcgtgc ccgtcggtca cggatcgcgg
     5221 ccacttcgct tacgcgtgcc agttgttccg gggtcagctt caggaaaggg ttgtcgaatg
     5281 ctccggatgc cggcaccaga tccgcccaca tgacctggcg cggttcctcg gcgagtacca
     5341 ccgtggcaag cagcgtgccg gccaggagcg agcaaaaaca tgtgcgcatg atcataggtt
     5401 cgtcttcccg cattcagatt ctaagcgtca tcccatcagc gagcgaataa cgatagatcc
     5461 gataggctgg aatcagcccg atcagcagcc ccgccattgc gacgagacca atcactcc
//
LOCUS       DATMZQ010000322         7586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1992349, whole genome shotgun sequence.
ACCESSION   DATMZQ010000322 DATMZQ010000000
VERSION     DATMZQ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992349"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="mch"
                     /locus_tag="VN496_10320"
     CDS             <1..755
                     /gene="mch"
                     /locus_tag="VN496_10320"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HXF17390.1"
                     /translation="RWPWRVSVHCSDPVLACLGSQYAGWSLAHGEGKGSFHALGSGPA
                     RAAACREDLFAELSYHDLAESVCLVLEVDKKPPLEITDKIARDCGVAPDQVTVILTPT
                     RSLAGTVQVVARVLEVALHKAHALGFALHKLVDGAGTAPLPPPAADFLTAMGRTNDAI
                     LFGGAVQLFVDCDDGEAEHLAVNLPSRVSRDYGKPFARVFKDAAYDFYRIDPHLFAPA
                     AVTVTALKSGNTFHAGAIDEQLLESSFGAGDG"
     gene            748..1665
                     /locus_tag="VN496_10325"
     CDS             748..1665
                     /locus_tag="VN496_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HXF17391.1"
                     /translation="MAEVIIFTDDPGWHGARLRDAFTARGVGTRFVSLRECGFDLHRP
                     GRHVAVPGCGDRLPDGAFVRGVPGGTLEQVVLRLDVLHALKLLGVPVYNDGRAIERTV
                     DKAMTSFVLASAGIATPATWVTESQSYAAEVVRAEIAAGHELVMKPVFGSQGIGLQRI
                     GGENELPAAAGYDGVYYLQRFIASGTEEWHDLRVFVIHGRAVAAMQRHGKSWITNVSQ
                     GGRCVPLDLSSNAEVGALAEKACRALHMDYAGVDLIRDGVGLLYVVEVNGIPAWKGLQ
                     SVTQPSIAAMLANDFTDRYLRRPALHSVA"
     gene            1662..2570
                     /locus_tag="VN496_10330"
     CDS             1662..2570
                     /locus_tag="VN496_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HXF17392.1"
                     /translation="MNAIHQFALERAAVSNAIHDACITDIRAFKPGNVSAGSPGHGMQ
                     ADDFIASATAMAREIAAPMADVGERILRAVEATRSVVSVNTNLGIILLCAPLVHAATA
                     RMAERRLRVRLHAVLVALDVYDAELAYRAIRLAAPGGLGRADRHDVAQSPSVTLSEAM
                     NEARERDRIAYQYVSDFEDIFETALPALRETHARWRSRDWAAVYVYLVMLARFLDSLI
                     VRKHGVEVAADVSRQAADLESALARVDDPSRELPALEAFDRQLKAKSINPGTTADLTV
                     AALVAMDLEDSLEKLSPASRAGSASG"
     gene            2635..3147
                     /gene="fae"
                     /locus_tag="VN496_10335"
     CDS             2635..3147
                     /gene="fae"
                     /locus_tag="VN496_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HXF17393.1"
                     /translation="MAKINGVCVGESLVGDGNEVAHIDLLIGPRGSPVETAFCNTLTN
                     NKDGFTSLLAVVAPNLLCKPATVMFNKVTIKGAKQAVQMFGPAQRAVAMAVADSVAEG
                     VIPADEADNLFICVGVFIHWQAADDKKIQDYNYKAVKESIARAIKGEPRASEVTAKRG
                     SMDHPFQAHK"
     gene            complement(3222..3944)
                     /locus_tag="VN496_10340"
     CDS             complement(3222..3944)
                     /locus_tag="VN496_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXF17394.1"
                     /translation="MVLANLHLIPVIDVLRGKVVHARQGRRAEYAPVESNLCHGSDPH
                     TIMAGLLKLHPFRTVYFADLDAIQNQGSNQETFARLRERHPAIEFWIDSAIANETALK
                     RSVVAGLGRAVIGSESLQQADFMQVARDLCHDNELILSLDFRGEAFIGPKPLLDYPER
                     YWPQRVLAMNLERVGSAKGPDFELIVNLAQRVPGCRVYAAGGVRSMEDLEHVAACGGA
                     GALIASALHDGRIGPSELKQFI"
     gene            4045..5025
                     /locus_tag="VN496_10345"
     CDS             4045..5025
                     /locus_tag="VN496_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXF17395.1"
                     /translation="MRILVYEYITGGGMLDDSNMTALAPEGDAMLRAVVDDLTDIPGT
                     EVTVLRDFRLKADFPARLRIVHSGQFAAVYRQALLECDAVWPIAPETDGILLRVTSEI
                     LATGRTLLGSRADAIAVAGSKQATAAALARAGVTVAAVYSGEPDIPACVEEIVAKPDD
                     GAGCQDTLLFRNREQLRSWCGQHARPKTVYQAYVPGEARSLSLLCCDGDARMLACNLQ
                     HVEIHEGAFRFNGVSVNAARAHPYSALASSIARAMPGLWGLCGVDFIETDAGPVVIEV
                     NPRLTTSYAGLHRALGLNPARLVLELPASLEARRDKELEIRLAEITAHAR"
     gene            5015..6061
                     /locus_tag="VN496_10350"
     CDS             5015..6061
                     /locus_tag="VN496_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXF17396.1"
                     /translation="MPGKHVLGWDIGGAHLKVAAVNSSGKVVRILCVPCPLWQGLDCL
                     DRALSAALKQMPTAALHAVTMTGELSDFFRNRAHGVKSIIARFVRQVPASVTVIYAAA
                     PGFLTTRQAVRKSALVASANWRASAELVAGLVPDGLFVDIGSTTTDVVPFRAGQLRNR
                     GLNDAERLARNELVYTGVVRTPIMALTQQIRFNGQWIGTMAEYFATTSDVYRLTGELP
                     KGADLLSTADNGGKSVRDSARRLARMIGRDVESAPLSRWRRCARDLADLHSGQIRKAC
                     ESLCSAANLPAGAPIIGAGTGRFLVKKLAKQLRRPYRDFHSLVNVSSRAAGRTSDCAP
                     AVAVALLAHGIRSQ"
     gene            6058..6687
                     /locus_tag="VN496_10355"
     CDS             6058..6687
                     /locus_tag="VN496_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXF17397.1"
                     /translation="MTAWVVKLGGSVYNSPELPKWLDVIAGTGAGKVILVPGGGPWAD
                     EVRTAQKIDGFDDRTAHRKALLAMEQYGQVLARMRSNLAPASSVAGIKQVLQQKQIAV
                     WMPYAMVVEEPSIEASWDVTSDSLAAWLTSRLDASGLMLIKSVQMDELQPGIAELVRL
                     GWVDQAFARFASGIHVPIIVFGLGGQQAALELLASPADENRFPVARLNI"
     gene            6846..>7586
                     /locus_tag="VN496_10360"
     CDS             6846..>7586
                     /locus_tag="VN496_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXF17398.1"
                     /translation="MKKSAVTPVCGPNPDKTLLRSLDDLPGPRGIPILGNALDIKPKE
                     LHRLLGGWADQFGPLFVFRLATQRLLTIVDAETIQHVLRERPELFRRWSRMEDIANDI
                     AANGLFTAEGAKWRRQRKFVMHALNASHVREFIPRLEQVVGRLRRRWWRAALSSASFD
                     AHVDLMRLTVDAASGLAFGRDLNTLEEKSDPIQNQLDKIFPAVARRLTALFPYWRYLP
                     LQADREVNDAVMQVGKLIDSLIAETRVRL"
BASE COUNT         1549 a         2142 c         2358 g         1537 t
ORIGIN      
        1 tgcgctggcc atggcgagtt tcggtgcatt gttcggatcc ggtactggcg tgcctgggca
       61 gccaatatgc cgggtggagc ctcgcgcacg gggaaggcaa gggaagcttc catgcgcttg
      121 gctcaggacc ggcacgcgcc gccgcatgcc gcgaggatct gttcgccgag ttgtcctatc
      181 acgatctcgc cgaatcggtc tgtctggtgc tcgaagtcga taaaaaaccg ccgctggaaa
      241 tcacggacaa gattgcgcgg gactgcggcg ttgcgccgga tcaggtgact gtgatcctga
      301 cgccgacccg cagtctcgcc ggcacggtgc aggtggtagc gcgcgtgctg gaagtggcat
      361 tgcataaggc gcatgcgctt ggatttgcgc tgcataaact ggtggacggc gcaggaacgg
      421 caccgctgcc gccaccggcc gctgatttcc tgaccgccat gggacgcact aacgacgcaa
      481 tcctgtttgg cggtgcagtt cagctctttg tcgattgcga cgatggggaa gccgagcacc
      541 tggcagtcaa tttgccgtcg cgcgtttcca gggattacgg caaaccattc gcacgcgtgt
      601 tcaaggatgc cgcttacgac ttttatcgca tcgatcccca tctgtttgca ccggcagccg
      661 tcacggtcac tgcgctgaaa agtggcaaca cgtttcatgc cggcgcgatc gacgaacagc
      721 ttcttgaatc gtcttttgga gccggcgatg gctgaggtaa tcatctttac cgacgacccg
      781 ggctggcatg gcgcgagatt acgggacgcc ttcacggcgc gaggcgtcgg cacacgtttc
      841 gtgtcgttgc gcgagtgtgg attcgatttg caccgccccg gacgccacgt cgctgtgccc
      901 ggatgcgggg atcgtttgcc cgacggagca ttcgtccgcg gggtacccgg gggtacgctg
      961 gagcaagttg tgttacgcct cgatgtactg catgcgctca agctgctcgg cgtgccggta
     1021 tacaacgacg gccgcgccat cgagcgcaca gtggacaagg caatgacgag tttcgtgctg
     1081 gcaagtgccg gcatcgcaac gccggcgacc tgggtcaccg aatcccaaag ctacgctgcg
     1141 gaagtcgtgc gcgccgaaat tgcggccgga cacgaacttg tcatgaagcc ggtgttcggg
     1201 tcgcaaggca tcgggctgca gcggatcggt ggcgaaaacg aattgccggc agcggccggc
     1261 tatgacggcg tgtactacct ccagcggttc atcgcgtccg gcactgaaga gtggcacgac
     1321 ttgcgcgtct tcgtaatcca cggtcgcgcg gtggcggcca tgcagcggca tggaaaaagc
     1381 tggattacca acgtatcgca aggcggccgt tgcgtgccgc tcgacttgtc atcaaacgca
     1441 gaggttggcg cgcttgccga gaaggcatgc cgggcattgc acatggacta cgcgggcgtt
     1501 gacctgatac gcgatggcgt gggactgctt tacgtcgtcg aagtcaatgg cattccggcg
     1561 tggaagggcc tgcagtccgt tacgcagccg agtattgctg cgatgctggc taacgatttc
     1621 accgacagat atctgcggcg cccggcgctg cattcggttg catgaatgca atacatcaat
     1681 tcgcactcga gcgtgcagcc gtctccaatg ccatccacga tgcctgcatc accgatattc
     1741 gcgcattcaa gccggggaat gtcagtgccg gctctcccgg gcacggcatg caggcggatg
     1801 actttattgc cagtgctact gcgatggcgc gcgaaatagc cgctcccatg gcggacgtgg
     1861 gcgaacgcat attgcgtgcg gtcgaggcga cgcgaagcgt tgtttcggtc aacacgaatc
     1921 tcgggatcat attgttatgc gcgccgctgg tccacgcagc aacggcccgc atggccgagc
     1981 gccgcttgcg tgtgcgactc cacgcggtac tggtcgcgct ggatgtgtac gatgcggaac
     2041 tcgcttatcg cgccattcgg ctggcagcgc ccggcggact gggacgagcc gatcgccatg
     2101 atgtcgcaca gtcgccaagc gtcacgctct cggaggcgat gaatgaggcg cgcgagcggg
     2161 acagaattgc gtatcagtat gtcagcgact tcgaggacat atttgaaacc gcattgccgg
     2221 cgttgcgcga gacccacgcg cgctggcgca gccgcgactg ggctgcagtg tatgtttatc
     2281 tggtcatgct ggcgcgcttc ctggacagcc tcatcgttcg gaaacacgga gtggaagtgg
     2341 cagccgatgt tagccggcaa gcagccgatc tcgaatcggc actggcacgt gtagatgatc
     2401 catcgcggga attgccggca ctggaggcgt tcgatcgcca gttgaaggca aagtccatca
     2461 atccgggcac gacagccgat ctgactgttg ccgccctggt tgcaatggac ctggaggatt
     2521 cgctggaaaa gctgtcaccg gcaagccggg cgggcagcgc cagcggatga gttcgtagtc
     2581 aacagcgggc attgcccgtg ttcataacca agtcaaaaac cgaaggagga gtatatggcg
     2641 aagattaacg gagtatgcgt cggcgaatcg ctggtcggcg atggcaatga agtcgcgcac
     2701 atcgacttgc tgatcggccc acggggaagt cccgtggaaa cggcattctg caatacactc
     2761 acgaataaca aggatggttt tacttcgctg ctggccgtcg ttgcgccaaa tctgctgtgc
     2821 aagccggcaa ccgtgatgtt caacaaggtc acgatcaaag gggccaagca ggccgtgcag
     2881 atgtttggac cggcacagcg cgcagtcgcg atggccgttg cggatagcgt ggcggaaggt
     2941 gtcattcctg cggacgaagc ggacaatcta ttcatctgcg tcggcgtatt tattcactgg
     3001 caagcggcgg atgacaaaaa aatccaggac tacaactaca aggccgtgaa ggaatccatc
     3061 gcgcgtgcca tcaaaggcga gccgagggca tccgaagtaa ccgcaaaacg cggttcaatg
     3121 gaccatccgt tccaggcaca caagtaaagc aagccctgca tacgcccgct ccgctgtcac
     3181 ggagcgggcg ttttgatcga aaatgcggaa gcgttgcgaa ttcaaataaa ctgcttcaat
     3241 tcggatggcc cgattcggcc gtcatgcagg gcgcttgcaa tcaatgcgcc ggcgccgccg
     3301 catgcggcaa cgtgttcaag atcctccatt gagcgtacac ctcccgctgc gtaaacacgg
     3361 catcctggca cgcgttgcgc gaggttgacg atcaactcga aatccggccc cttcgcgctg
     3421 ccgactcgct ccaggttcat cgccaatacg cgctgcggcc aatagcgttc cggataatcc
     3481 agcaatggtt tcggaccgat aaaggcctca cccctgaaat ccagcgaaag gataagctcg
     3541 ttgtcatggc aaaggtcacg agccacctgc ataaaatcgg cctgctgcag cgattcgctg
     3601 ccaataacag cgcggccaag tccggcgaca accgaccgct ttagagctgt ctcgtttgca
     3661 attgcactat caatccagaa ctcgattgct ggatggcgtt cgcgcaagcg cgcaaaggtc
     3721 tcttgattgc tgccctgatt ctggatggcg tcgagatctg cgaaataaac cgtccggaac
     3781 ggatgcagtt tcagcagacc ggccatgatt gtatgcggat cagatccgtg acataaattg
     3841 gattcaaccg gcgcgtattc tgcgcggcgg ccttggcgtg catgcacaac cttgccacgc
     3901 agaacgtcga tgacaggaat cagatgcaag tttgccaaaa ccattgttca gggtaaaagg
     3961 acaggaaaag cggctcgcgc cggacacggg ccattcgggt tcggcgtgcc atcatgtaat
     4021 tttagtctgc gaggtaattg gtccatgcgt attctggtct atgaatacat cactggcggc
     4081 ggcatgctgg acgattcgaa catgacggcg ctcgcgcctg aaggcgatgc gatgctgcgt
     4141 gcggtggtgg atgatctcac ggacatcccc ggcacggaag taacggtctt gcgtgatttc
     4201 agattgaaag cagatttccc ggcaagactg cgcatcgtgc attccggcca gtttgctgcg
     4261 gtgtaccgtc aggcactgct ggagtgcgat gcggtatggc ccatagcgcc tgaaacggac
     4321 ggcatcttgc tacgggttac gagcgaaatc ctggccaccg gtcggacgct gttaggcagc
     4381 cgtgctgacg cgattgctgt tgcaggaagc aaacaggcca ccgctgctgc gcttgccagg
     4441 gccggggtca cggtcgctgc agtgtattcg ggcgaaccgg acattcctgc gtgtgtggaa
     4501 gaaattgtgg ccaagccgga cgacggcgct gggtgccagg acacgctgct gtttcgcaat
     4561 cgcgagcagc ttcgcagttg gtgcggccag catgcacggc ccaagaccgt gtaccaagcg
     4621 tatgtgccgg gggaagcacg aagtctatcg ctattgtgct gtgacgggga tgcgcggatg
     4681 ctggcgtgca atcttcagca cgtggaaatt cacgaaggag cgttccgctt caacggtgtg
     4741 agcgtcaatg cggcgcgcgc ccatccgtat tcagcgttgg cgagttccat cgcgcgcgcc
     4801 atgcccggcc tatggggtct gtgcggcgtg gatttcatcg aaaccgacgc aggacccgtt
     4861 gtaattgaag tgaacccgcg actgaccact tcgtacgccg ggctgcatcg cgcgcttggc
     4921 ctgaatcccg cgcggctcgt gttggagcta ccggcctcac ttgaggctcg tcgggacaaa
     4981 gagctcgaaa tccgcctcgc ggaaataacg gctcatgcca ggtaaacatg tattgggttg
     5041 ggatattggc ggcgcacatt tgaaggttgc cgctgtaaac agcagcggta aagtggtgcg
     5101 gatcctatgc gtgccttgtc cgctgtggca ggggctggac tgtctcgacc gtgccctctc
     5161 ggccgcgctc aaacagatgc cgacagccgc gctgcacgca gtcaccatga caggcgaact
     5221 gtccgatttc tttcgcaatc gggctcacgg cgtgaagtcg atcattgcgc gattcgtaag
     5281 gcaggtcccg gcttctgtta ccgtcatcta tgccgctgcg cccggctttt tgacaacacg
     5341 gcaggccgtc cgcaaaagcg ctctcgtcgc atcggcgaac tggcgggcta gcgcagaact
     5401 ggtcgccggt ttggtgccgg acggcctgtt cgtcgatatc ggcagcacca cgaccgatgt
     5461 agtgccgttc cgcgccggcc aactccgtaa ccgcggctta aacgacgccg aacgattggc
     5521 ccgaaacgag ttggtgtaca ccggcgtagt ccgcacgccg atcatggccc tgacccagca
     5581 gatccggttc aatggacaat ggatcggcac catggccgaa tacttcgcta ccacttcgga
     5641 tgtctaccgc ctgaccggcg aactgcccaa gggagcagac ctgttatcta ctgccgacaa
     5701 tggtggcaag agcgttcgcg acagcgcgcg ccggctcgct cgcatgattg gccgggatgt
     5761 ggaatccgcg ccgctgtcac ggtggcgccg ctgcgcccgg gatctcgcag acttacattc
     5821 aggacaaatt aggaaggcat gtgaaagcct gtgctcagcc gcaaatctgc ctgcgggagc
     5881 gccgatcatt ggggctggaa caggaaggtt tctggtgaag aagctcgcga aacagctgcg
     5941 taggccctat cgggacttcc actcactggt caatgtgtcg tcgcgtgcag ccggccgaac
     6001 gtcagactgt gcgcccgccg tagctgtcgc gctgctggca cacgggatcc gcagccagtg
     6061 acagcctggg tcgtgaagct tggcggcagc gtgtacaact ctcctgagtt gccgaagtgg
     6121 ctcgacgtca ttgcgggaac cggagcgggg aaagttatcc tagtgccggg tggcggtccg
     6181 tgggccgatg aagtgcgcac ggcccaaaaa atcgacggct tcgatgatcg aacagcgcac
     6241 cggaaggcct tgcttgcaat ggagcaatac ggacaagtgc ttgccagaat gcggtcgaac
     6301 cttgcgccgg cgtcctctgt cgccgggatc aaacaagtct tgcagcaaaa acaaatcgcc
     6361 gtgtggatgc cgtacgccat ggtcgtggag gaaccctcca tcgaggcaag ttgggacgta
     6421 acctctgaca gtcttgcggc gtggctgact tcgcgattgg acgcttctgg attaatgctg
     6481 atcaagtcgg ttcagatgga tgaactgcag ccgggcatcg cagagctggt gcggctcgga
     6541 tgggtggatc aagcatttgc gagatttgcg tccggcatac acgtgccgat catcgtgttt
     6601 ggcctcggcg gccaacaggc agcgcttgaa ctgctcgcga gtcctgctga cgagaaccgc
     6661 tttccagtgg cccgactgaa catttgaact tatcaattga tagatttggc gcttgcatgc
     6721 gcacgagcga atcattgcgc gcttgatatc tcgtggactt tctttataaa ctccggactg
     6781 cgtttgcgag caaggtatgc acgtcgcatg atggaaagtg tttcgcccgg aattgaggag
     6841 actgaatgaa aaaatcagca gtgacgccgg tctgcggacc gaacccggat aagacactgc
     6901 tgcgctctct tgacgatcta cctggtccac gcggaattcc gatcctcgga aacgcactcg
     6961 acatcaaacc gaaggaactc catcgtcttc tcggcggctg ggccgatcaa tttggtccgc
     7021 tgttcgtttt tcggctcgcc acacagcgcc tccttaccat cgtggatgcc gaaaccatcc
     7081 agcatgtgct tcgagagagg ccagagcttt ttcggcgctg gagccggatg gaagacatcg
     7141 ctaacgatat cgcagctaat ggtttgttca ccgcggaggg cgccaagtgg cggcgccaac
     7201 gcaaatttgt aatgcacgcg ctaaacgcaa gccacgtacg cgaattcatt cctcgtcttg
     7261 aacaagtggt tggcaggctg cgccgccgct ggtggcgtgc cgcgctctcc tcggcatcgt
     7321 ttgacgctca tgttgatctg atgcggttga cggtagatgc cgccagcggg ttggccttcg
     7381 gcagggatct caatacgctg gaagaaaaat cagacccaat ccagaaccag ctggacaaga
     7441 tattcccggc agtcgctcgt cggcttacgg cgcttttccc atattggcgt tacctgccct
     7501 tgcaggctga cagagaggtc aatgatgcgg ttatgcaggt gggcaaattg atcgatagcc
     7561 tgatagcaga gacccgagtg cgcctt
//
LOCUS       DATMZQ010000323         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4062427, whole genome shotgun sequence.
ACCESSION   DATMZQ010000323 DATMZQ010000000
VERSION     DATMZQ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4062427"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VN496_10365"
     CDS             <1..194
                     /locus_tag="VN496_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17399.1"
                     /translation="NPFAPPKENLAVVAPAPLEAKARLASEQVDDYLMAHQQFSSGAT
                     LPGVASYVRMVSGDMEEKH"
     gene            196..1179
                     /locus_tag="VN496_10370"
     CDS             196..1179
                     /locus_tag="VN496_10370"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HXF17400.1"
                     /translation="MMHRALWLPALFALGLSGGVTRADSLQQSEGLALLQKIADAARE
                     LNYAGTFVYQHGDQVETSRIVHFVEDGNEYEKLETLDGPRREVIRNNDEVLCYYPDAK
                     VVRSDKRVARRSFPALLPEQLSSLTDYYEIRKGEAERIGGLNSQALVLEPKDGLRYGH
                     KFWADTASGLLLKARMLSERRNVVEQFSFTQLTIGSAVTRDMVQPSFNIRLPEWRLDR
                     YANATISEPESGWTVKNFPAGFRKILEMRRTKQGNAALVTHLVYSDGLAAVSVFIEPA
                     ANRNRISEGLSQQGAINIYTRTINDQLVTVLGEAPAATVIQIANSLVPRSR"
     gene            1245..2627
                     /locus_tag="VN496_10375"
     CDS             1245..2627
                     /locus_tag="VN496_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXF17401.1"
                     /translation="MLRTMVFSLLLWMPVLGMAQVQGLPDFSELVDKQGPAVVNVSTT
                     STARSGPAQGQVPEDDPFYDFFRRFGPPQPRDYETRSLGSGFIVSADGYILTNAHVVD
                     MADEVTVKLNDKREFKAKVIGADKRTDVAVIKIEASGLPAVRIGDPEKLRVGEWVLAI
                     GSPFGFESTVTAGIVSAKGRSLPQENYVPFIQTDAAINPGNSGGPLFNVKGEVVGINS
                     QIYSRTGGFMGLSFAIPIDVAMDISNQLRTTGKVSRGRIGVVIQEVTKELAESFGLPK
                     TSGALVNSVEKGAPADKAGIEASDVILKFDGKTVTSSSDLPRIVAQTRPGSKVIAQVW
                     HKGASRDVTITVAEMPEEKVAQRPARKDPKPANVVARLGMTLSELSPDQRKELGSSGG
                     LLVEDAQGPAAKAGIRRGDVLLAINNQDVKSVEQLTQLLNQFEKARSVALLIRRGDGS
                     LYVPLRLDGG"
     gene            2772..>3226
                     /locus_tag="VN496_10380"
     CDS             2772..>3226
                     /locus_tag="VN496_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXF17402.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRFIQLCGGLSDREMEEQVLDSM
                     DLERERGITIKAQTAALRYKARDGQVYQLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTAIEQGVEVVPVLNKIDLPSADPERVIAEIEDIIG"
BASE COUNT          713 a          909 c          943 g          661 t
ORIGIN      
        1 ataatccgtt cgcaccgccc aaggaaaacc tcgctgtcgt tgcacccgct ccgctggagg
       61 ccaaggcccg gcttgcaagt gagcaggtcg acgattatct gatggcgcat cagcagttct
      121 cgtcaggagc gaccttgccg ggcgtcgcct cttacgtgcg aatggtttcg ggggatatgg
      181 aagaaaagca ctgacatgat gcaccgggcg ctctggttgc ctgcgctgtt tgcgcttggc
      241 ctgtccgggg gcgtcacacg cgcggattcg cttcaacaat cggaaggcct ggcgctgctg
      301 caaaaaattg ccgatgcggc gcgcgaactg aactacgcgg gcacattcgt gtatcagcat
      361 ggcgaccagg ttgaaacatc gcgcatcgtt catttcgtcg aagatggcaa cgagtacgag
      421 aagctggaaa cgctcgatgg cccgcggcgc gaagtcattc gcaacaacga cgaagtgctc
      481 tgctattacc ccgatgccaa agtcgtgcgt agcgacaagc gcgtcgcccg gcggagtttc
      541 cccgcactcc ttccagaaca actttcttcg ctgaccgatt attacgaaat tcgcaaaggc
      601 gaagccgagc gtatcggcgg cttgaactcc caggcgttgg tcctggaacc caaggatggg
      661 ctgcgttacg gacacaagtt ctgggccgat acagcgtccg gattgcttct taaagctcgg
      721 atgctgagcg aacgccgcaa cgtcgtggaa cagttcagct tcactcaact caccatagga
      781 tccgcagtaa cgcgggacat ggttcagccg tccttcaata tccgattgcc ggaatggcgc
      841 ctcgaccgct atgcaaatgc caccatcagc gaaccggaat ccggatggac agtcaagaat
      901 tttcccgcgg gattccgcaa aatcctcgag atgcgccgca ccaagcaggg caacgccgcg
      961 ttggtcacgc acctggtcta ttccgacggc ctggctgccg tttcggtatt catcgagccc
     1021 gccgcaaacc gcaaccgcat cagcgaaggc ttatcccagc aaggcgccat caacatctac
     1081 acgcgcacga tcaacgatca attagtgacc gtcctgggcg aagcgcccgc tgcgacggtc
     1141 atacagattg cgaattccct ggtgccgcgt tccaggtaat ttcgcaacgc gcgctgagct
     1201 attatgctgt tgacgttcac tgttcatcac ctaggaaacc gctgatgctt agaacaatgg
     1261 tattttccct gttgctgtgg atgcctgtgc tgggcatggc ccaggttcag ggtttgccgg
     1321 attttagcga actggtggac aaacagggac cggcagtcgt caatgtgagc acgacttcga
     1381 ctgcacgcag cggtcccgct cagggtcagg ttccggagga cgacccgttc tacgactttt
     1441 tccggcgttt cggcccaccg cagccccgcg actatgagac gcgttcactg ggttccggct
     1501 ttatcgtcag tgccgacggc tatatcctga ccaatgcaca tgtcgtggac atggcagacg
     1561 aagtcacagt taaattgaac gacaagcgcg agttcaaagc caaagtcatc ggtgctgaca
     1621 aacgtaccga cgtggccgtg atcaaaattg aggcaagcgg gttacccgct gtccggattg
     1681 gtgatcccga aaaattgcgg gttggcgaat gggtactggc gatcggttct ccctttggct
     1741 tcgaaagtac cgtcactgcc ggcattgtca gtgccaaagg gcgctcgttg ccgcaagaga
     1801 attacgtacc gttcatccag actgatgccg ccatcaatcc tggcaattcc ggtggccctt
     1861 tgttcaatgt gaaaggtgaa gtcgtaggca tcaattccca gatttacagt cgcaccggcg
     1921 gattcatggg cttgtctttc gcaattccca ttgacgtggc aatggacatc tccaatcagc
     1981 ttcgcaccac gggaaaggtc agccgcggtc gcattggcgt cgtgatccaa gaggtcacca
     2041 aggagctcgc ggagtcattc gggttgccca agacatccgg cgccctggtc aattcggtgg
     2101 aaaaaggcgc accggcggac aaggcgggca tcgaggcttc ggatgtcatt cttaaattcg
     2161 atgggaagac tgtcacatcg tcatcggact tgccgcgcat cgtggcccaa acccggccgg
     2221 gctcaaaagt aatcgcgcag gtgtggcaca agggggcttc ccgcgacgta accataacgg
     2281 tggcagaaat gccggaggaa aaagttgctc agcgcccggc gcgaaaagat cccaagccgg
     2341 caaatgtggt tgcaaggctc ggcatgacct tgagcgagct cagcccggat caacgcaagg
     2401 aacttggatc cagcggtgga ctgctggtcg aggatgctca aggccccgcg gccaaggccg
     2461 gcattcgccg cggcgacgtg cttttggcca tcaacaacca ggacgtaaaa tcagtcgagc
     2521 aactcaccca gctgctgaac cagttcgaga aggcgcgaag tgtcgcattg ctgatcaggc
     2581 gtggcgatgg gtcactgtac gtgccgttgc gccttgacgg aggctgagca gcaagaagcg
     2641 gtgcggtcgc acgaagagca gggcgctggt ctgaatgggg gcgagaaagc cgctcgccgc
     2701 ggtgcaaaat ccgctacaat ttcgccctct ggaattcaca tgaaagggca cgctcgtgcc
     2761 cttttttact gatgcagcat atccgcaatt tttcgatcat tgcccacata gaccatggca
     2821 aatccacact tgcggaccgg ttcatccagc tttgcggcgg actttccgat cgtgaaatgg
     2881 aggagcaggt gctcgactcg atggatctgg agcgtgagcg cggcataacc atcaaggcgc
     2941 aaactgctgc tttgcgctac aaggcacgtg acggccaggt ctaccaactc aatctgattg
     3001 atacgccggg tcatgtcgat ttttcctacg aagtaagccg gtcattggca gcctgcgaag
     3061 gcgcgctgct ggtggttgac gcttcccagg gcgttgaagc gcagaccgtc gcgaattgct
     3121 ataccgccat agagcagggc gtcgaagtgg ttccggtgct caacaagatt gatttgcctt
     3181 cagcggatcc ggagcgggtg atcgcggaaa tcgaggacat catcgg
//
LOCUS       DATMZQ010000324         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_612165, whole genome shotgun sequence.
ACCESSION   DATMZQ010000324 DATMZQ010000000
VERSION     DATMZQ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612165"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VN496_10385"
     CDS             <1..1325
                     /locus_tag="VN496_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HXF17403.1"
                     /translation="FSRDETPWKGVNRLVEAHVIEVGRAGPQAREYWQPDLFSPLSYR
                     SDDEYADHYRHLFTDSVRRTARSHRKLAVEVSGGLDSSAILAVAENLRRRQELPAPGI
                     AAYTLKFDEDPEANELEYARAVSVHVGIPVHEVSPMYNPLSWYRTWAKTYKEFPGYPN
                     GVMAMGLREVARDEGCRVVLSGVGGDEWLWGNRAYYAEELASGRWRSLYECVRTDCRE
                     AGAMNALWWLGRHAAMLLPRCAEETLRRMRAVFSVRVNDRKAWLTPKLRKIASQRKAD
                     LALSFESRRFGRAGQRIQYETLYSAYSVIARELDERAAASVGMEVRLPFWNTAMVQFA
                     FSTPERLRLRGGTPKILHRRAMAGLLPGNVLERKDKSDFMVTFRRHADAINSDRSLDM
                     RREQHEWVLESRTMEMYNQFGNRQYEGEPEWMLWSLFGWSALASGLEI"
     gene            1379..1552
                     /locus_tag="VN496_10390"
     CDS             1379..1552
                     /locus_tag="VN496_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17404.1"
                     /translation="MKPKQAPKTTTASVASRDKAHKKAYQQPALRALGALHLLTRSGV
                     SSMNGDGGALMMP"
     gene            1558..2676
                     /locus_tag="VN496_10395"
     CDS             1558..2676
                     /locus_tag="VN496_10395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF17405.1"
                     /translation="MLEEHLEYVSDALRLEYFRSAIGKTVKRGGRVADLGSGSGILGL
                     LCAQAGAAHVHFIDDSAMIDIARETVQRAGFGDRASFIKGRSQRVDLPERVDVVICDH
                     VGHFGFDYGIAGLFQDAKRRFLKPGGTLIPGRIKLHIAALQSEQCQLLAQAWKARDVP
                     DEYRWLRRFSVNCKHSVNVKRDELLGAPAEMGGIDFHDDQPDFFSWTAEMRIEREGVM
                     HGLVGWFDCELAEGAWMTNSPLAEKPIRRRQAFMPVGDPVPVRSGDLVKARVMARPDD
                     NQIAWIAEFPATGLRRSHSTWEGMLISPVDLFMSEPNRVPRPSREGIARMSVLGYCDG
                     RRTVREIERAILRHHADLFPSPAEISRFVAHVLGRDTV"
     gene            2679..>2995
                     /locus_tag="VN496_10400"
     CDS             2679..>2995
                     /locus_tag="VN496_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17406.1"
                     /translation="MEFSLQDARAQPAMDIAPFHEWSFPDGALWTQFYRTKEGYLLRF
                     PGLADFQVSADDLAVTCHPAPEATGETSRHLYLNQVLPLVLSKLGKLVFHASAIEVEN
                     GAVA"
BASE COUNT          598 a          833 c          976 g          588 t
ORIGIN      
        1 ggttttctcg cgatgagacg ccatggaaag gcgtcaaccg gctggttgag gcgcatgtaa
       61 ttgaagtcgg gcgcgcggga ccgcaagcgc gcgaatactg gcaacccgat ctcttttcgc
      121 cgctgtcata ccggagcgat gacgagtacg ccgatcatta ccgccatctc tttactgact
      181 cagtccggcg cacggcgcgc tcccacagga aacttgcagt cgaagtaagc ggcggcctcg
      241 actcttcggc gatacttgcc gtcgccgaaa atctgcgacg gcggcaggaa cttccggccc
      301 ccggcatcgc ggcttacacg ctcaagttcg acgaggatcc tgaagccaac gaattggagt
      361 acgcccgcgc tgtcagcgtc cacgtcggca ttccggtgca cgaagtgagt cccatgtaca
      421 atccattgtc gtggtaccgg acctgggcga aaacctacaa ggaattcccc ggctatccga
      481 acggcgtaat ggcaatggga ctcagggaag tcgcccggga cgaaggatgc cgggtcgtgc
      541 tgagcggtgt cggcggcgac gaatggttgt ggggaaaccg ggcctattac gccgaggaac
      601 tggcttccgg tcggtggcgc agcctgtatg aatgcgtgag aacggattgc agggaggccg
      661 gtgcaatgaa cgctctgtgg tggctgggac ggcatgctgc gatgttgttg ccgaggtgcg
      721 ccgaggaaac gttgcgccgg atgcgtgcgg tattcagcgt ccgcgtgaat gaccgaaaag
      781 catggctcac gccgaaattg agaaaaatcg cttcgcagcg gaaagcggat ctggcgcttt
      841 catttgagag ccgccggttc ggacgcgccg gacaacgcat ccagtacgag acgctgtatt
      901 cggcttattc cgtgatcgcg cgggaactcg acgagcgcgc agcggcgagc gttggcatgg
      961 aagtgcgcct gcctttctgg aacacggcga tggtgcaatt tgccttttcc acgccggagc
     1021 ggctccggct acgtggcggg acgccgaaaa tcctgcaccg gcgtgccatg gcggggctgc
     1081 ttcccggaaa tgtgctggaa cgcaaagaca agtcggactt catggtgact ttcaggcggc
     1141 acgccgatgc gatcaacagc gacagatcgc tggatatgag gcgcgaacag catgaatggg
     1201 tgttagagtc gcgaaccatg gaaatgtata atcagttcgg caatcggcag tacgaaggcg
     1261 agccggagtg gatgttatgg agcctttttg gctggagtgc gctggcgtcc ggcctggaaa
     1321 tttaggcgtg cgttgcgcac cttgagcggg accagaagga tctaatatgg agacgccaat
     1381 gaaaccgaaa caggcaccga agacaacaac ggcgagtgtg gcgagccgcg ataaggcgca
     1441 taagaaagcc tatcaacagc ctgcgctgcg cgcacttggc gcgcttcatc ttctgacccg
     1501 gtctggcgtt tcatcgatga acggagatgg cggcgcgctc atgatgccgt gagcagcatg
     1561 ctggaagagc atctcgagta tgtctcggat gccttgaggc tcgagtattt caggtctgcc
     1621 atcggcaaga cagtgaaacg cggcggtcgc gtcgccgatc tcggcagtgg ctccgggata
     1681 ctggggctgc tgtgcgcgca ggcaggagcc gcgcatgttc acttcattga cgacagtgcg
     1741 atgatcgaca tcgcacgcga gaccgtgcag cgcgcgggat ttggcgatcg cgcctcgttt
     1801 atcaaggggc gatcacaacg cgtcgatctg ccggagcggg tcgatgtcgt catttgcgac
     1861 cacgtcggcc atttcggctt cgattacggc attgccggcc ttttccagga cgcaaagcgg
     1921 cgttttctga agcccggcgg tacgttgatc ccgggacgga tcaagctgca tatcgccgcg
     1981 ctgcagtccg agcagtgcca attgctggcg caagcctgga aagcccgcga tgttccggac
     2041 gaatatcgct ggctgcgccg gttttcggtc aactgcaagc attcggtcaa cgtgaagcgc
     2101 gacgaactgc ttggcgcacc cgccgagatg ggcggcattg atttccacga cgaccagccg
     2161 gatttctttt cctggacggc cgaaatgcgc atcgagcgcg aaggtgtcat gcatggactg
     2221 gtgggctggt ttgactgtga attggccgaa ggcgcctgga tgacaaattc gcccctggcg
     2281 gagaaaccga tacggcgccg tcaggccttc atgccggtcg gcgaccctgt tccagtcaga
     2341 tcgggggacc tggtgaaggc aagagtgatg gcccggcctg atgacaacca gatcgcatgg
     2401 attgcggagt ttccggccac cggcctgcgc cgcagccatt cgacctggga aggaatgttg
     2461 atctctcccg tggatctctt catgtcagaa ccgaaccgtg tgccgcgccc aagccgggag
     2521 ggaatcgcgc gaatgagcgt gcttggctac tgcgacggca ggcgcacagt ccgggaaatc
     2581 gagcgggcca tattgcggca ccacgccgac ctttttccgt ccccggcgga aatttcgcgc
     2641 ttcgtcgcgc atgtgcttgg cagggatacg gtttagccat ggagttcagc cttcaggacg
     2701 cgcgcgcgca gcctgccatg gacatcgcgc cgtttcatga atggtcgttt ccggacggtg
     2761 cattgtggac acagttctac cgaacaaaag agggttacct gttgcgcttt cccggattgg
     2821 ctgatttcca ggtttccgcg gacgaccttg ccgtaacctg ccacccggcg ccggaggcga
     2881 ccggcgaaac atccaggcat ctttatctga accaggtgct gccgctcgtc ttgagcaagc
     2941 tgggcaagct ggtttttcac gccagcgcga tcgaggttga aaacggggcg gtcgc
//
LOCUS       DATMZQ010000325         2456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3372753, whole genome shotgun sequence.
ACCESSION   DATMZQ010000325 DATMZQ010000000
VERSION     DATMZQ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3372753"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /gene="ccoP"
                     /locus_tag="VN496_10405"
     CDS             <1..598
                     /gene="ccoP"
                     /locus_tag="VN496_10405"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HXF17407.1"
                     /translation="QTAAAKNYGPLFAKYAAMEIPLVAADPQAREIGQRLFLNYCSQC
                     HASDARGGRGFPNLTDGDWLYGGDPAIIQATIMSGRNGIMPPLGAALGEEAVKNVANY
                     VLLLSGSEHDAALAAKGKETFTTICAACHGVDGKGNQALGAPNLTDRVWLYGGNLTTI
                     SETIRNGRNNKMPAWGEFLGDEKTHLLAAYVWGLSNGK"
     gene            588..1187
                     /locus_tag="VN496_10410"
     CDS             588..1187
                     /locus_tag="VN496_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HXF17408.1"
                     /translation="MASDKVGYAAHYEQQVTGWRNGSAQYGSLSIALHWVMLLLLIAV
                     YATIELRGMFPKGSDPREAMKAWHFMLGLAVFGLVWLRLLIQATAGPVPAIVPVQPRW
                     QALSAKLMHVLLYLFMIAMPILGWLVLSAEGKPIPFFGVNLPALIAENKESAKSLQEI
                     HETVGELGYWLIGVHAAAALFHHYVVRDNTLSRMLFARR"
     gene            1189..>2456
                     /gene="ccoG"
                     /locus_tag="VN496_10415"
     CDS             1189..>2456
                     /gene="ccoG"
                     /locus_tag="VN496_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HXF17409.1"
                     /translation="MQGNVTPPLAVPAAPVEQALYEIRKKIYPRAVTGTFALWRWLFV
                     WGTQVVFYGVPWLQWNGRQAVLFDLAHRKFYIFGLVFWPQDIFYLTVLLIISAYSLFL
                     FTAVAGRLWCGYACPQTVYTEIFLWIEKIIEGDRLKRMKLDGSRLSVRKVALKAAKHA
                     AWIALALWTGFTFVGYFTPIHEVSRETFALALGPWESFWILFYGFATYGNAGWMREQV
                     CKYMCPYARFQSVMFDPDTLVITYDRERGEPRGARSRSADQKAMGLGHCVDCGICVQV
                     CPTGIDIRNGLQYECIGCAACIDGCDQVMDKMGYPKGLIRYSSETALKRHLSLRDMMR
                     RVLRPRVLLYTGILWAIILVAAIALYQRVPVKLDVIRDRIATAQGSDENEVENVYRLQ
                     IMNTSEIPRRFLVTVSGPDSLELEGGHEPV"
BASE COUNT          461 a          683 c          766 g          546 t
ORIGIN      
        1 gcagacggcc gcggcgaaga actacgggcc actgtttgca aaatacgcgg ccatggagat
       61 tccattagtt gcggctgatc cccaggcccg cgaaattggg cagcggttgt ttttgaatta
      121 ttgttcgcaa tgccatgcct ccgacgcgcg tggcggacgc ggatttccga atctgaccga
      181 tggcgattgg ctgtatggcg gcgacccggc catcatccag gccaccatca tgtcggggcg
      241 caacggcatc atgccgccgc ttggtgcggc gcttggcgaa gaggccgtca agaatgtcgc
      301 caactatgtg ttattgctat ccgggtcgga acacgatgct gctctggcgg cgaaaggcaa
      361 ggaaacgttt acgacgatct gcgccgcatg ccatggcgtg gacggcaaag gcaaccaggc
      421 gctcggcgca ccgaatctga ctgaccgtgt ctggctctac ggcggcaacc ttacaaccat
      481 ttcggaaacc atccgcaatg gccgcaacaa caagatgcct gcgtggggtg aattcctcgg
      541 agacgaaaaa acccatcttc tcgccgcgta tgtgtggggc ctttcgaatg gcaagtgata
      601 aggtcggcta cgctgcgcac tatgagcaac aagtgactgg ctggcgcaac ggcagtgcgc
      661 agtatggttc gctgtcgatt gcgctgcact gggtgatgct gttgttgctg attgcggtct
      721 acgcgaccat tgagctgcgc ggaatgtttc cgaagggaag cgacccacgc gaagccatga
      781 aggcgtggca tttcatgctg ggtttggctg tcttcgggtt ggtttggttg cgactcctca
      841 ttcaggcgac ggccggcccg gttcctgcca tcgtgccggt ccagccgcgc tggcaagccc
      901 tatcggcaaa attgatgcat gttctgcttt atctgttcat gatcgccatg ccgatattgg
      961 gctggctggt gctgagcgcg gaaggcaagc caattccgtt cttcggcgtc aacctgcccg
     1021 cgttgattgc cgaaaacaaa gagtccgcca agtcgctcca ggagattcac gagaccgttg
     1081 gtgaattggg ctactggctc atcggcgtgc atgctgctgc cgcgctgttc catcactatg
     1141 tcgtgcggga caacacgctg agccgcatgt tatttgcccg gcgctgacat gcaaggcaac
     1201 gtaaccccgc cgctcgccgt accggcggcg cccgtggagc aggcgctcta cgaaatccgc
     1261 aagaaaatct atccgcgtgc ggtcaccggc acctttgcgc tctggcgctg gctgttcgtc
     1321 tggggcacgc aagtggtgtt ttatggcgtg ccgtggctgc agtggaatgg ccggcaggct
     1381 gtgctgttcg acctggcgca ccgcaagttc tacatcttcg gcttggtatt ctggccgcag
     1441 gatatttttt acctgacggt actgctgatc atttcggcgt actcgctgtt tctgttcacc
     1501 gcggtggcgg gccgcctctg gtgcggctat gcgtgtccgc aaacggtcta taccgaaatc
     1561 tttttgtgga tcgagaaaat cattgaaggc gaccggctca agcgcatgaa gctggacggt
     1621 tcgcggctat cggtccgtaa ggtagccctg aaagctgcaa agcacgcggc gtggattgcg
     1681 ctggcgctgt ggaccggttt taccttcgtc ggctatttca cgccgataca tgaagtcagc
     1741 agggaaacgt tcgcgctggc tctggggccg tgggagtcgt tctggattct gttctatggc
     1801 tttgccacct acggcaatgc cggctggatg cgcgagcagg tctgcaagta catgtgtccg
     1861 tatgcgcgct tccagagcgt gatgttcgac ccggatacgc tcgtcattac ctatgaccgc
     1921 gaacgcggcg agccgcgcgg cgcacgttcc cgcagtgccg accagaaggc aatgggtctg
     1981 ggccattgcg tcgattgcgg catctgcgtg caagtctgtc ccacgggcat cgatatccgc
     2041 aacgggcttc agtatgaatg catcggctgc gccgcctgca tcgacggctg cgatcaggtc
     2101 atggacaaga tgggctatcc gaaaggcctg atccgttact cgagcgagac tgcactcaag
     2161 cgccatttgt ctctacgcga catgatgcgt cgggtcctgc gaccgcgcgt gctgctgtat
     2221 accgggattc tttgggcaat catcctggtt gcggccatag cgctgtatca gcgggtcccg
     2281 gtaaaactgg atgtgatccg cgaccgcatt gcgaccgctc agggcagcga tgagaatgaa
     2341 gttgaaaatg tctaccgcct gcagatcatg aacacatcgg aaatcccgcg gcgttttcta
     2401 gtcacggtga gcgggccgga ttcgctggag ttggaaggcg gccatgagcc ggtaga
//
LOCUS       DATMZQ010000326         3956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3029442, whole genome shotgun sequence.
ACCESSION   DATMZQ010000326 DATMZQ010000000
VERSION     DATMZQ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029442"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VN496_10420"
     CDS             <1..1593
                     /locus_tag="VN496_10420"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF17410.1"
                     /translation="TVRILMDFQTRDRETHCSEMTRVVAFGSRAKLGYAAEPYPTLPF
                     SECNDMSPFLSAKKLASAIRRKQIRSVELLDSYLERVRRLNPQLNAIIVLDEKRAREQ
                     ARKADRAAAKGEWNGPLHGVPMTVKESFDLEGHPTTWGRTDMKGNIAKKNALAVQRLM
                     DAGAVVFGKTNVPALLADWQTFNPVYGTTNNPWDVTRGPGGSSGGAAAALAAGLTGLE
                     LGSDIGASIRNPAHFCGVFGHKPTFRLCSSTGHNLPPNLAVKDMAVIGPMARSADDLE
                     LALKLIAQPDEVEARGLRVALAKPKPDVKKLKIAVLTSAATAEVDDSVQAAVRAAANA
                     FAKRGAKISETARPDFDLHEAHRTFIHLLRGATSSALSEEQFARQRELAANLKPQEEG
                     YAAWMLHANTMPVREWHSWNEKRERVRHAWATFFREWDLLLCPTAATAAFEHNQKGER
                     WERMVMVNGKPQPSTTQMFWAGFPGMAYLPSTIAPAGFTREGLPMGVQIVGPQYGDLA
                     TIAAAQFLEREFQPFVAPKGYE"
     gene            complement(1626..3065)
                     /locus_tag="VN496_10425"
     CDS             complement(1626..3065)
                     /locus_tag="VN496_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF17411.1"
                     /translation="MDEQRLVPVNDFARFDAIELADLVRRKEVSATELVEAAIARIER
                     LNPHINAVVATMYADARRIARGRLPDAPFAGVPFLLKDSLALYAGVPTTSASSVLRSR
                     VAGHDSALVARLKRAGLIILGKTNCAEFGLLPTTEPRMFGATRNPWNPQRSPGGSSGG
                     SAAAVAAGMVTMAHGTDGGGSIRIPASCCGVFGLKPTRARNPCGPHYGDIPGRLVVEH
                     AITRSVRDSAALLDATARPELGDPYCVPAPPRSFLQEADVHPGRLRIAFSAPSVADLH
                     PDCAAALQDAALVCRDLGHQVINAGPRIDYLRAAHLFTTLWAAGCAANLDGIAHCRGM
                     RIASGEVEPVTWFLYETGSHISASEYQSAICELQDMARSVARFFSDFDVWLTPVVTEP
                     PPLLGSFDAPSEDLFAAFRRAWAFAPIPAICNVTGQPAMSVPLYWNAEGLPIGSHFAG
                     RFGDEATLFRLAAQLEQCRPWKARSPRLI"
     gene            complement(3058..>3956)
                     /locus_tag="VN496_10430"
     CDS             complement(3058..>3956)
                     /locus_tag="VN496_10430"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17412.1"
                     /translation="YDALIVSVAQGKHLHYVSALGFGLGYLGGGLLFALNVAMTLWPE
                     RFGLADSTQAVRVSFVMVAAWWVVFSVPLLVFVREPAGPSTHGVCRVVREGLRQFAAT
                     FRHVRNLREVWLFLLAYWLYIDGVYTIARMALDYGMALGFDPKSLIVALLITQFVGFP
                     AALAFGYAGERFGAKPAIFVSIGTYAGVTVCSYAMTEVWEFYALATVIGLVQGAVQSL
                     SRSLYAQIIPTAQAGEFFGFYNMAGKFATVLGPLLMGITASLTGSSRVAILTILILFV
                     VGAALLCLVREGDNQPFPNRNG"
BASE COUNT          888 a         1187 c         1183 g          698 t
ORIGIN      
        1 actgtccgga ttctaatgga cttccagacg cgcgatcggg aaacgcactg cagcgagatg
       61 acgcgcgttg ttgcctttgg atcgagggcc aagctcgggt atgctgcaga gccctatcca
      121 actttgcctt tttcagagtg caatgacatg agccccttcc tgtccgcgaa aaaacttgca
      181 tcggctatcc gccgcaaaca aattcgcagc gttgagctgc tcgattctta tctggagcgc
      241 gtcaggcggc tgaatccgca gctcaacgcc atcatcgtgc tagacgaaaa gcgggcacgc
      301 gagcaggcgc gcaaagctga tcgcgccgct gccaagggtg agtggaacgg cccattgcac
      361 ggagttccga tgacggtgaa agaatccttc gatcttgaag gtcaccccac tacctggggg
      421 cgaacggaca tgaagggcaa catcgcaaaa aaaaatgctc tcgccgtgca gcgcttgatg
      481 gacgccggcg cggtcgtgtt cggcaagacc aatgtgccgg cgctgctggc agactggcag
      541 acgttcaatc ccgtctacgg cacgaccaac aacccgtggg atgtcacgcg cggtccgggg
      601 ggctcttccg gcggcgccgc ggcggcgctg gcggcaggct tgacgggact cgaactgggc
      661 agcgatatcg gcgcctccat tcgaaatccc gcgcatttct gcggcgtatt cggccataag
      721 ccgaccttcc ggctgtgctc gagcaccgga cataatttgc ctccgaacct ggcggtcaaa
      781 gatatggcgg tcattggtcc aatggcgcgc agcgcggatg acctggagct agcgctgaaa
      841 ctgattgcgc aacccgacga ggtagaggcg cgcgggctga gagtcgcgct ggcgaagcca
      901 aagccagacg taaagaaact gaagatcgcc gtgctgactt cagcggcgac ggcggaagtc
      961 gacgattcgg tacaagccgc ggtacgggcc gcggcaaatg catttgcaaa gcgcggcgca
     1021 aaaatcagtg aaacggcgcg gccggatttt gacttgcacg aggcccaccg cacttttata
     1081 cacttgctgc gcggggcaac ttcgtctgcg ctcagcgaag aacagtttgc acgccagcgt
     1141 gaactcgccg ccaatctcaa accgcaagag gaaggttacg cggcctggat gctgcacgcg
     1201 aatacgatgc ccgtgcggga gtggcatagt tggaacgaaa aacgcgagcg ggttcgccac
     1261 gcgtgggcca cattcttccg cgaatgggat ctgctgctct gcccgacggc cgcgaccgcc
     1321 gcgtttgagc acaatcagaa aggcgagcgc tgggagcgca tggtgatggt caacggcaag
     1381 ccgcaacctt cgaccacgca aatgttttgg gccggcttcc ccggcatggc ttacttgcct
     1441 tcgacaattg cgcccgcagg attcacgcgg gaaggattac ccatgggcgt acagattgtc
     1501 ggaccgcaat acggcgatct ggcgaccatc gccgccgcgc aattcctgga acgcgagttc
     1561 cagcctttcg tcgctccaaa agggtacgaa tgattcagga cgaccatgca acaaccaggt
     1621 tcaggtcaga tcagtctcgg actgcgcgcc ttccatggac ggcactgttc cagctgggcc
     1681 gcgagccgaa atagtgttgc ctcgtcgccg aaacgaccgg cgaaatgcga tccgatggga
     1741 agaccctccg cattccagta aagcggtacg gacatggccg gctgccccgt gacattgcag
     1801 attgccggaa tcggcgcaaa ggcccaggct cggcggaatg ccgcgaacag gtcttcagat
     1861 ggcgcatcaa agcttccgag caatggaggt ggctcggtca ccaccggcgt cagccagacg
     1921 tcgaagtcgg aaaaaaaccg ggcaacgctg cgcgccatgt cctgtaattc gcaaatagcc
     1981 gactgatatt cggatgcgct tatgtggctg cccgtttcgt acagaaacca ggtaactggt
     2041 tcgacctcac cgctggctat gcgcatgccg cggcaatgag caatgccgtc aaggttcgca
     2101 gcacaaccgg cggcccacaa tgtcgtaaac aagtgagccg ctctcagata atcgatgcgc
     2161 ggaccggcat tgattacctg atggccgaga tcgcggcaaa cgagtgcggc atcctggagc
     2221 gcagcggcgc aatccgggtg caggtcagcg acgctcggtg ctgaaaatgc aatgcgcaat
     2281 cgccccgggt gaacgtccgc ttcttgcaaa aaactccgtg gcggcgcagg cacacaatag
     2341 ggatcgccca actccggcct tgcagtggca tcgagcaggg ctgcgctatc gcgcaccgat
     2401 cgcgtgatcg catgttcaac caccagccgg ccagggatgt cgccatagtg cggtccgcat
     2461 ggatttcgtg cgcgtgtggg tttcaggccg aacacgccgc agcatgatgc ggggatgcgg
     2521 atggatcccc ctccatcagt cccgtgcgcc attgtgacca tgcccgcagc aaccgccgca
     2581 gccgagccgc cgcttgaccc cccaggcgag cgctgcggat tccagggatt gcgtgtggcg
     2641 ccaaacatcc tgggttcggt ggttgggaga aggccgaatt ccgcgcagtt cgttttgcca
     2701 aggatgatta atcccgcccg tttaagccgc gccaccagtg cgctatcgtg tccggcgacc
     2761 cggctacgca gcaccgacga tgctgacgtc gtcggtacgc ccgcgtacaa cgccaggctg
     2821 tccttgagta aaaaaggtac accagcaaac ggcgcgtcag gcagacgacc ccgcgcaatt
     2881 cttctggcat ccgcatacat tgtcgcgacc accgcattga tgtgcggatt cagccgctcg
     2941 atgcgcgcga ttgcggcctc gactaattca gtcgcggaaa cttccttgcg ccgaacaagg
     3001 tccgccaatt cgatagcgtc gaaccgcgcg aaatcgttta ccggcaccaa gcgctgttca
     3061 tccattgcga ttagggaagg gttgattgtc tccttcccgc accaggcaca agagcgctgc
     3121 tccgaccacg aacaaaatca ggatcgtgag aatagcgact cgcgaactgc cggtaaggga
     3181 ggcggtgatc cccatcagca acgggccgag cacggtggcg aatttgccgg ccatgttata
     3241 gaagccgaag aactcgcctg cctgggccgt tggaatgatt tgcgcataaa gcgagcgtga
     3301 taaggattgc accgcacctt gcaccagtcc gattaccgtt gcgagtgcat aaaactccca
     3361 aacttcagtc atggcatagc tgcataccgt tacgccggcg taggtgccga tggacacgaa
     3421 gatggcgggc ttggccccga agcgttctcc cgcgtatccg aatgcaagcg cggcgggaaa
     3481 gccgacaaat tgcgtaatca gaagcgccac gatcaggctc ttcggatcga agccgagggc
     3541 catgccgtag tccagcgcca tgcgtgcgat ggtataaacg ccatcgatgt aaagccagta
     3601 agccagcaag aataaccaga cttcgcgcaa atttctcaca tgccgaaagg tggcggcgaa
     3661 ctggcgcagg ccttcccgca ccacgcggca aacgccgtgc gtggaagggc ccgcaggttc
     3721 tctgacgaag accagaagcg ggacggaaaa cacgacccac cacgcggcga ccatcacaaa
     3781 tgaaacgcgc accgcttgcg tactgtcggc aagtccgaaa cgttctggcc agagtgtcat
     3841 tgcgacgttc agtgcaaaca acaacccgcc ccccagatat cccagcccaa acccaagcgc
     3901 ggatacatag tgcaaatgtt ttccctgggc gactgaaacg atcagtgcgt cataga
//
LOCUS       DATMZQ010000327         3685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1517556, whole genome shotgun sequence.
ACCESSION   DATMZQ010000327 DATMZQ010000000
VERSION     DATMZQ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517556"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VN496_10435"
     CDS             <1..283
                     /locus_tag="VN496_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXF17413.1"
                     /translation="GAKGEGQGRVPAVWGLNSFNAGAGLNNVQKLAGFIWANMPMGNE
                     RSLTYQEALDVAAFINLQYRAFDPREGRLKKLAERIYHRLTLIFGKDNP"
     gene            309..1805
                     /locus_tag="VN496_10440"
     CDS             309..1805
                     /locus_tag="VN496_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXF17414.1"
                     /translation="MSLLREMLPDLSWWRSADWRREAIAGWTGALLVIPQAITFAYLS
                     GVAPEYGLYCAVFVALLSSLFGNSPMVGGPNTAVAILLGLTVLPFAGRGSPLFTELVL
                     VLSLVVGLIQILIWLLRGAELFRYFSPAAITGIKAGVGVLLITSALEGGLGLSPMTTN
                     FFYEKFYIALVSWQDLVNPYAATVSGVTIAAGLLLKKNWPRSYIIGAVLIGTLVGAGI
                     YGLLGPVQSQLELLGHVTLRGLPLNVPRIGPQHWLFLEQVVPQAMAIAVLGLAQSLVI
                     ARDLKARAARDLDLHKEVFAQGISNALSPFFSAFAGSGSFNRTSVALEMGARTPASGI
                     VAAFAVVVIAWALGPLLVWLPMPVIAGVMTLVGIGMIQGKEIRPLLNRVDGPVFLITV
                     LAIIFVSLEIGILVAVAVSVGFFVASASKVKLAVSREGDKEHIVVSGNLFYASLDHLA
                     KQLRSDPSTRTILDLGRVPYCDASALALFESIRRERQRCGGQLDVVAA"
     gene            1984..3591
                     /locus_tag="VN496_10445"
     CDS             1984..3591
                     /locus_tag="VN496_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17415.1"
                     /translation="MKHIALMALRVFLVLGMPLVAQAHEDESIPTGAPDKLGAVMFPV
                     SCSPAAQEQFNRGLAMLHSFFYPEAAKTFTKATELDPSCAMGHWGVAMSWWYPLWYPP
                     TRDSFNRGKAALEKAVMTGAPTERERAYIAALGEFYGDFEKADNKTRTLAYEKAMGAL
                     VQKFPEDLEAATFYALALQATANPNDKTYANQLKSAGILEKIFAEYPEHPGVAHYLIH
                     AYDYPELAPRALKAARLYGAIAPSMPHALHMPSHTYIALGLWQDSIESNLAASAAARK
                     LGWIPEELHSKDYLVYAYLQLAQSASAKDMLQKIAEVKIDESARTLPVDYAVAASPAR
                     YVLEQQRWEEAARLQPQPSRFPATQALTHFARAIGAAHLGATSEAEKEIAQITQIRDG
                     LVQSKQDYWVKQVEVQRQAAQAWLAWASGDNAQAVEQMRSAVALEESTYKHPITPGQL
                     LPARELMGDLLMAIGQPGQALTEYEASLRSNPNRFNGIFGAARSAELAGFGSKAADYY
                     RKLLDLGQTAESERKEIRQAKLFLSNR"
BASE COUNT          706 a         1081 c         1150 g          748 t
ORIGIN      
        1 tggcgcaaaa ggcgaaggtc agggccgggt tcctgccgta tggggcctga actcctttaa
       61 cgctggcgcg ggactcaaca acgtccagaa actggccgga ttcatctggg ccaacatgcc
      121 gatgggcaac gagcgcagtc tgacttacca ggaggcgctg gacgtggctg cgttcatcaa
      181 tctgcagtat cgcgcgttcg acccgcgtga agggcggctc aagaagctgg cggagaggat
      241 ataccaccgt cttacgttga tattcgggaa ggacaatcca tgacggcgcc ggcgttgccg
      301 ggactgccat gagcctgctg cgcgaaatgc tgcccgatct ctcgtggtgg cgatcagccg
      361 actggcggcg cgaagcgatt gcaggatgga ccggagcgct tctggttatt ccccaggcca
      421 tcacgtttgc ctacctctcg ggcgtcgcgc ccgaatacgg actgtactgc gcggtattcg
      481 tagccttgct gtcgagtcta ttcggcaact caccgatggt cggcggcccc aataccgcag
      541 tggcgatcct gctgggcctg accgtgctgc cctttgccgg tcgcggcagc ccgttgttta
      601 cggagttggt cctggtactg agcctggtgg tcgggctgat ccagatcctc atctggttgc
      661 tgcgcggtgc ggagctcttc cgctacttca gtccggcggc gatcaccggc atcaaggcgg
      721 gcgttggcgt actgctgatc acctcggcgt tggaaggcgg gctgggcctg tcgccgatga
      781 cgacgaattt cttctatgaa aagttctaca ttgcgttggt gtcgtggcaa gacctggtca
      841 atccgtacgc agcaaccgtc agcggtgtca ccatcgcggc gggcctgctg ttgaagaaaa
      901 actggcctcg cagttacatc atcggcgctg tgctgatcgg aaccctggtt ggcgcaggga
      961 tctatgggct gcttggaccc gtgcaatctc aactggaact gctggggcat gtcaccctgc
     1021 gcggcttgcc tttgaacgtg ccgcgaattg gaccgcagca ttggctgttc ctggagcagg
     1081 ttgtgccgca ggccatggcg atcgcggtac ttggcctcgc gcagtcgctg gtgatcgcgc
     1141 gcgatctgaa agcgcgcgcg gcgagagatc tcgacctgca caaggaggtc tttgcgcaag
     1201 gcatctcgaa cgcattgagt ccgtttttct cggccttcgc gggttccggc agtttcaatc
     1261 ggaccagcgt tgcgttggaa atgggcgcac gcacacctgc ctcgggcatc gtcgcggcct
     1321 ttgccgtggt cgtgatcgca tgggcgctgg ggccgcttct ggtctggttg ccgatgccgg
     1381 tcatcgccgg cgtcatgacg ctcgtgggca tcggcatgat ccagggcaaa gaaatccggc
     1441 cgttgctgaa ccgcgtagac ggcccggtat tcctgatcac cgtactggcg atcattttcg
     1501 tgagcctgga gatcggcatc ctcgttgccg tggcagtctc ggtgggtttc ttcgttgcca
     1561 gcgcgtccaa agtgaaattg gcggtttcgc gcgaaggcga caaagagcac atcgtcgtca
     1621 gcggcaatct cttctacgca tcgctcgatc atcttgccaa gcaactacga agcgatccat
     1681 ccacgcgcac catcctcgat ctgggtcgtg ttccctattg cgatgcgtcg gcgctggctc
     1741 ttttcgaatc aatccggcgg gaacgacagc gctgcggtgg ccaactggat gtcgtcgccg
     1801 cttgataaat cgtcccgcgc atgactccgt cgcgcaggcc gagtagtatg ggccgggctg
     1861 cccgtcgcac gcatgactcc cggcgcggcg gaatgtggca ctgttgtttg gaggtatatc
     1921 gatagaatgg tagatcgcgt atccgcttct tcccgggcgt gctcacctga ctaaggaatc
     1981 gagatgaaac atatcgcatt gatggcgttg cgcgttttcc tggtgttggg tatgccgctg
     2041 gttgcgcagg cgcacgagga cgaatcaata ccgaccggcg cgccggacaa actgggtgcg
     2101 gtcatgtttc cggtttcctg cagccccgct gcccaggaac agttcaaccg cggcttggcg
     2161 atgctgcatt cctttttcta ccccgaggcg gcgaagacct tcaccaaggc gaccgaactg
     2221 gatccctctt gcgcaatggg tcactgggga gtcgccatga gttggtggta tccgctgtgg
     2281 tatcccccaa cccgggattc gtttaaccga ggcaaagccg ctctggagaa ggcagtcatg
     2341 accggggcgc ccacggaacg cgaacgtgcc tacattgcgg cgctcggcga gttctatggc
     2401 gacttcgaga aggcggacaa caaaacgcgc acccttgcct atgaaaaggc catgggcgca
     2461 ctggtccaga agtttcccga agacctggag gccgcgacgt tctacgctct tgcgcttcag
     2521 gctactgcga atccaaacga caagacgtac gcaaatcagc tcaagagcgc cggtatcctc
     2581 gagaagatat tcgccgagta tccagaacat cccggcgttg cacattatct gatccatgca
     2641 tacgattatc cggagctggc accacgggcg ctcaaggccg cgcgcctcta tggtgccatc
     2701 gcgccgagca tgccgcacgc gttgcacatg ccatcccaca cctatattgc gcttggcctg
     2761 tggcaggact cgatcgagtc caatctcgcc gccagcgccg cggcccgtaa gctaggctgg
     2821 attccggaag aacttcacag caaggactat ctggtgtacg cgtacctgca gcttgcccag
     2881 tccgcatcgg ccaaagacat gttgcagaaa atcgccgagg tcaagatcga cgagagcgca
     2941 cgcaccctgc cggtcgatta cgccgttgct gcctctccgg cgcggtatgt actcgaacag
     3001 cagcgctggg aagaggcggc cagactccag ccgcagccga gtcgctttcc tgcgacccag
     3061 gcgttgacgc attttgcgcg cgcgattggg gcagcgcatc ttggtgcgac gagcgaggcg
     3121 gaaaaggaaa tcgcgcaaat cacgcagatt cgcgacgggc tcgtgcaatc gaaacaggat
     3181 tactgggtca agcaggtcga ggtgcagcgc caggcggcgc aggcgtggct ggcatgggcg
     3241 agtggtgaca acgcgcaggc agtcgagcag atgcgcagtg ctgtcgcgct cgaagaatcc
     3301 acctataaac acccgatcac accgggacag ctactgccag cgcgagaact gatgggagac
     3361 ctgctcatgg cgatcggtca gcccggtcaa gcgctgacag agtacgaggc ttcgttacgc
     3421 tccaacccga atcgcttcaa cgggattttt ggcgccgcac gctcggccga attggcgggg
     3481 ttcggaagca aggccgcgga ctactaccgc aaactgctgg accttggaca gacggcggaa
     3541 agcgaacgca aggagatccg ccaggcgaag cttttcctgt cgaacaggtg atcgcaggaa
     3601 tatctccggt ggcatgggca aaaatttgga tcagattgcc agcggacaat tttctttcgg
     3661 tagtaccacc gacatgcagg ccccg
//
LOCUS       DATMZQ010000328         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3375065, whole genome shotgun sequence.
ACCESSION   DATMZQ010000328 DATMZQ010000000
VERSION     DATMZQ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3375065"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..535)
                     /locus_tag="VN496_10450"
     CDS             complement(164..535)
                     /locus_tag="VN496_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXF17416.1"
                     /translation="MTIKNELYGDVLVLSPEGRIDHANSEEFRREVGPFIDRCKKDGQ
                     TIVLDLNGVDYISSAGLRCFMLAEKQAHAQGGTIVVAAMQPVVQEIFEISRFTLVFET
                     FATVGDALSRFSPAAFKAYQA"
     gene            complement(676..1899)
                     /locus_tag="VN496_10455"
     CDS             complement(676..1899)
                     /locus_tag="VN496_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXF17417.1"
                     /translation="MKSHSSNMKTWTRREILRAGLTSIGAGAMPLLFSRAMAAEAKPS
                     TRILVVLELSGGNDGLNTLVPYHDDAYYRHRPKIGIPPAKLRKIDDRYGFNAGMAGFE
                     RLYKDGRMAIVHGCGYAQPSFSHFTSMAYWHTGAPNRGEEYGWVGRVADAIDRKSTPN
                     FIVNIDEVQSLAVRAREHVPVVFDDPEKFGRKGLYQERALLDGVPDMERGSSPSKEYL
                     IEVAKSAREASQLVREAWSSYKTPVDYGIVPVQLPKVASLIAAGMPTRLYYTSYRHNA
                     FDTHVQQSDLHQRLLTYVSDAVQGFMKDMDRIGRGQDVTVLVFSEFGRRVPENTSLGT
                     DHGTANHMYIIGKAVTGGHYGIPVSLTNLDEGDNLIFTTDFRRVYATVIGGWLGCRTT
                     DQLLSGNFESFSVFS"
     gene            complement(1958..>3399)
                     /locus_tag="VN496_10460"
     CDS             complement(1958..>3399)
                     /locus_tag="VN496_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HXF17418.1"
                     /translation="IRVKPTGNRRLQPVANKFFYWLRASRLETNRVAYWWANRMLATQ
                     RPLEEKMTLFWHGYFATSEEKVRDYRKMLKQNELFRAQGAGNFRDLLIAVAQDPAMLA
                     FLDAGVNVKGAPNENFAREIMEMFSMGVGNYSEADIREAARAFTGWNFVDLDFVVTPE
                     KHDASVKTFFGKQGHFNGIDIIDLILQQPVAGEHVAGKIYRFFVRDDLPPELRAELGI
                     RLRKSNYEIAPFLETLFLSRDFYSTASMAVHIKSPVELVISTYRKMELKEVPGVPDFN
                     EVTEALGQKLFFPPTVAGWANGRSWITPGLLLARGNFVYDTLFPDINFLPPDRYPLTD
                     YKIGTVNEKLALGLDVTTATKPDAKEVTSMSMQADRDEDFNTRLASFRGWQMALQKVK
                     PIPRATAQVDLSAMVLAAGCKNARQAADYLIARFLSVQVNAQVHERIAKFLEGELGTA
                     DITAAASFLEEPLRSTLHVILSLPEYQLG"
BASE COUNT          810 a          952 c          870 g          767 t
ORIGIN      
        1 aaaaaaaaag atcgtcgttc aaaaaggagc gtagtttctc cggtgaatcg actcctttgg
       61 agatagccag ggaaagtgcc gactcgatct tgcgacggat atcggcagag gtaagggcga
      121 ctggaatgga accgcgagtc ccccagaagc ggaccagcat cctctaggcc tgataggcct
      181 tgaacgccgc gggcgaaaat cttgacagtg catcgcccac ggtggcgaaa gtttcgaaaa
      241 ccagtgtgaa gcgactgatc tcgaaaattt cctgcaccac cggctgcatg gcagctacca
      301 ctatcgtgcc gccttgcgca tgcgcttgct tctccgcgag cataaagcag cgcagtcctg
      361 cactgctgat gtaatccacg ccattgagat ccagtactat cgtctgccca tccttcttgc
      421 accgatcgat gaaagggccc acttctctgc gaaactcttc gctattggca tgatcgatcc
      481 gcccctcggg cgaaagcacg agcacatcgc cgtaaagctc atttttgatt gtcattcagt
      541 cttcccgtcc ggacaataac caccaacccg catgccagct gatgatttac cgattcagca
      601 ggttgcctca caggggcaaa gcaattcagc gaaggccgta ttaaacctga aaatacagca
      661 tgtcggtgtt caagatcagg aaaagaccga aaaactttca aagtttccgg acaacaattg
      721 atcggtcgtc cggcaaccaa gccaaccgcc aattacggtg gcataaactc gccgaaaatc
      781 cgtggtgaaa atcaggttgt caccctcgtc cagattagtc aggctaaccg gtatcccgta
      841 gtgccctccc gtcaccgcct tgccgattat atacatgtgg tttgcggttc catgatcggt
      901 gccgagactg gtattttccg gcacgcgacg gccgaactcc gagaagacaa gtaccgtcac
      961 atcctggcca cggccaatac ggtccatatc cttcatgaaa ccctggaccg catcggagac
     1021 gtaagtcaga agtcgctgat gcagatccga ttgctgcaca tgggtgtcga aagcgttgtg
     1081 acgatatgac gtgtaataga ggcgagtcgg catacctgcc gcaatgagtg atgcaacttt
     1141 agggagttgt accggcacaa tgccgtagtc cactggagtc ttgtagctgg accaggcttc
     1201 acgtaccagc tgggaagctt cccgggcgct ttttgccacc tcgatcaaat actcttttga
     1261 gggactggag cctcgctcca tgtctggcac gccatccagc agggcacgct cctggtatag
     1321 ccctttgcgc ccgaatttct cgggatcatc gaatactacc gggacatgct cgcgggcccg
     1381 cacagcgagg gattgcacct cgtcgatatt gactatgaaa tttggagttg attttctgtc
     1441 gattgcgtca gctacccgac ccacccagcc atactcctcg ccgcggttcg gtgcaccagt
     1501 gtgccaatag gccatcgaag taaagtggga gaacgacggt tgcgcatagc cgcacccgtg
     1561 cacgatcgcc atcctgccat ctttgtacag ccgctcgaat ccggccatac cggcgttgaa
     1621 gccatagcga tcgtcaatct tgcgtaattt cgctggagga attccgattt tcgggcggtg
     1681 acgatagtaa gcgtcatcgt gataaggaac cagtgtattc agtccgtcgt tgccaccaga
     1741 caattcaaga acgaccaaga tacgcgtaga gggcttcgcc tccgcagcca ttgcacgcga
     1801 aaacagcaga ggcatcgcac ccgcgccgat acttgtcagt ccagcgcgca gtatctcgcg
     1861 ccgcgtccag gttttcatgt tcgaagaatg gcttttcatg atgtgttttt ccgagcaatt
     1921 gcggacaaag tcgagatcca gtgaccgcga agagtattca tccgagctgg tattcgggca
     1981 aagaaagaat cacatggagc gtgctgcgca gcggctcttc tagaaagctc gcagcagccg
     2041 ttatatcagc agtgccaagt tctccttcca ggaactttgc aatgcgctcg tgcacttgag
     2101 cattcacttg caccgacaag aatcgcgcta tcagataatc agctgcctgc cgtgcgttct
     2161 tgcaccccgc ggccaggacc atggcagaca ggtcaacttg ggccgttgcg cgcgggatag
     2221 gcttaacctt ctgcaatgcc atctgccagc cgcggaagct cgcgagccgc gtattgaaat
     2281 cctcatcgcg atccgcctgc atcgacatgg aagtaacttc cttggcatcc ggcttggtag
     2341 cggtagtcac atcaagaccc agcgccaatt tctcgttaac agtcccgatc ttgtaatcgg
     2401 tcaatggata gcggtcaggc ggcaaaaaat taatatccgg gaaaagtgtg tcgtatacaa
     2461 aatttccgcg ggccaacaac aggcctggcg tgatccagct tctcccattt gcccagccag
     2521 ctacagtagg tgggaaaaac aacttttgcc caagggcttc cgtaacctca ttgaagtcgg
     2581 gtacgccggg cacttccttc aattccattt tcctgtaggt ggaaataacc agttccacgg
     2641 gtgattttat atggaccgcc atagaggccg tactatagaa atcgcgcgac agaaacaagg
     2701 tttccaggaa gggtgcaatt tcgtagttcg attttcgaag ccggatcccg agttccgcac
     2761 gcaactccgg cggcaagtcg tcacgcacga aaaaacggta aatcttcccg gcaacatgct
     2821 cgccggctac tggttgctgc aaaatcaggt caatgatatc gatgccattg aagtgacctt
     2881 gcttgccgaa aaaagtcttg actgaagcat cgtgtttttc gggcgttacg acgaaatcga
     2941 gatcgacgaa attccatccg gtaaaggcgc gcgctgcttc acgtatatcg gcttcgctgt
     3001 aattgccgac acccatggag aacatttcca tgatttcacg cgcgaagttt tcgttgggtg
     3061 cgcccttcac attgaccccg gcatcgagga atgcaagcat tgccgggtcc tgcgcaactg
     3121 caatcagcaa atcacgaaag ttacctgccc cctgcgcgcg gaacagctcg ttctgcttga
     3181 gcattttgcg atagtcacgg accttttctt cgctggtggc gaaatagcca tgccagaaca
     3241 gcgtcatctt ctcttccaga ggacgttgag tggcaagcat gcggttcgcc caccaatatg
     3301 cgacccggtt tgtttccagc cggctcgctc tgagccagta aaaaaatttg ttagccactg
     3361 gctgcaaccg gcggttgccc gttggcttca ccctgatac
//
LOCUS       DATMZQ010000329         9685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3375579, whole genome shotgun sequence.
ACCESSION   DATMZQ010000329 DATMZQ010000000
VERSION     DATMZQ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3375579"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VN496_10465"
     CDS             <1..1138
                     /locus_tag="VN496_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXF17419.1"
                     /translation="KPEHHSRRLPEANGPWSLGLELEIPLVAQGKRAAQAERGAFLAD
                     AADLDLAHAVWLTRSRVRDQYLDLRGSREALVSLDAQLAARQEMIGLVNRRVEAGMLS
                     ARELAAERVAYSQIEQSRNQELTKQQRANAALAAALGMPMEVLTSMALRFDAPLPEPL
                     ELDAGTLRGMALRNRLDVHRKLLEFGAADADVKFAVAAQNPDITFGPGYMWDQGDNIW
                     SLAVGISLPPAARTRAMIRETQARRELAAEQFAAVQLQVIGLVASTGAQYRLARERAS
                     AAERQLQTQREQEFRIERQFAAGSADRMQRLAARIETITAEAVLQSAQVAQRQALAQM
                     EDAVQRPLLGDLALPPVPGPRTSDMDNGMANHSRHQDRPQASRL"
     gene            1135..2196
                     /locus_tag="VN496_10470"
     CDS             1135..2196
                     /locus_tag="VN496_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17420.1"
                     /translation="MKGRLAYAIIALMGLVIAALSWALVYYSRDELGTQTEKRDDRVT
                     SASVAGIESGRAIVRLSAQSQAAAGIVVQPLKSATHESALEAYGIVANLQPLAEWRGR
                     YLSAAAEARAARATLAAAESEYRRMQLLFQDDRNVSEQAMQAALARYRAEQLRVSAAD
                     QAAASIRDGLRGAWGESVTQWAVNPDSQTMRSLLQQTSFLVQLVVPNQLPRGALRNRI
                     AIAPAIARENVRRATFVGASPQIDAAFPGETYFYIVNGGGLRAGMRVVARFGMGGSAT
                     GVIVPSAAVVWHAGKAWAYVKDDDDSFARFEVLTADEMEGGWFNTAGFDDDDEVVVSG
                     AQLLLSEELKYQIRNENED"
     gene            2289..5399
                     /locus_tag="VN496_10475"
     CDS             2289..5399
                     /locus_tag="VN496_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF17421.1"
                     /translation="MISTIVRQSIRYSGVVVALALTIVVYGLFTLSRAHLDVFPEFSP
                     SQVVIQTEAPGLSAELVEKQVSQPIENALSGVSGLDNLRSQSIPGLSVLHVFFQEGSD
                     IYRNRQVVSERLGSVVAQLPKGIVPVMTPLTSSASTVLGLGVTSSQRSLMELRTLVDW
                     TIRPHLLAVRGVAEVNVFGGDVRQWQIQVDPEKMHRFGMALDDIVFAARRATGVAGAG
                     FVKTPNQHLLLQVDVQPDAVTDVAHAVLAYRDGRAVRIGDVADVTEGAAPSIGAAAID
                     GTPGVFMLVQGQLGSNTHAVTQDLETAVAEIRPLLEKDGIKLYPRLFRPANFIETAVR
                     NVQRDILIGSLLVVVVLFLFLFNTRTAFICATAIPVSLLAAVLVLDFFNVTFNIMVMG
                     GLAIALGEVVDDAIIDTENIFRRLRLNRSLSTPRPVSEVVLAASIEVRSSVVYATFIV
                     ALVFVPLLTLGGVAGKLFAPLGLAYIFAILASLLIAVTLTPALCYLLLAGAELKADDP
                     PLVRWMKPKYLFVLGRIERFPGRVIAVTAIVIAIGIAFLPLFAGQFIPALREGHYILH
                     MSAVPGTSEAESLRIGRRVSQAIGSIKGVQSVAQWVGRAKNGADTFGTHYSEFEVEIG
                     PLPGKEQVRILEEIRQTLSGKRGGGFPGVTFAVNTFLTERIEETITGYAAPFVVNLYG
                     SSLDLLDRDALALASVLAGVPGARDVQIQAQPGAPQLSIRLRHERVAALGLQPLAVLE
                     TVQTAYEGTQAAQIFRGNQVIDLVVVLDPAHRDRIHDIAALPLRNPDGMLVRLGDIAD
                     IQIISGRYKILHDSGRRLQTVTADVVGRDIEQFERDARARIAAAGTLSEGNYAVFAGT
                     AQAQVQARSDLLLHAALAAIGIFLLLYIAFNNLHNLLITSINLPFALIGGVIAVVASG
                     GWMSLGSLVGFVTLFGITLRNSIMLVSHYQHLVQVERMPWNADTALRGAAERLPSILM
                     TALVTALGLMPLAVGSGEPGREIEGPMATIIVGGLATSTLLNLLVLPAILLHFGRFKS
                     SAAGRA"
     gene            5478..5873
                     /gene="trxA"
                     /locus_tag="VN496_10480"
     CDS             5478..5873
                     /gene="trxA"
                     /locus_tag="VN496_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXF17422.1"
                     /translation="MSTIALTKDNFEQVVTGNDTVIVDFWAPWCGPCRSFAPVFEATS
                     EKHEGVVFAKVNTEEEPELAGHFEIRSIPTLMVFREKVLLFAQPGALPASGLEAVIQK
                     VKAVDMAEVHRDIATQEAAAGAEKANTPS"
     gene            5883..6293
                     /locus_tag="VN496_10485"
     CDS             5883..6293
                     /locus_tag="VN496_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17423.1"
                     /translation="MAPAFLDETSFDSFVSAHDTAVIGFIQQNTDAAKFSALADAVAA
                     KYPGVAFAQVGASSRTLFETFGIGRTATAIFRERIGMYLEPGFPAAELLDRMLLSITA
                     LDMEHVRGELEQEKAARESLAVHRVCPATRRGKL"
     gene            complement(6300..7232)
                     /locus_tag="VN496_10490"
     CDS             complement(6300..7232)
                     /locus_tag="VN496_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXF17424.1"
                     /translation="MRISLDALLVLDSIARNGSFAAAGEELHRVPSAITYTIQKLEQD
                     LDVQLFDRSGHRARLTEAGEALLREGRHLLRTATDLECRVKHVATGWESELRIAVDDL
                     VPIERLFPLLKDFYREQSGTRIRLSTEVLGGCWDALIGGRADMVIGAPGDAPPEGLLA
                     VEPIGSFEFVFVVSPDHPLATAKEPLAAQEIRQHRAISAADTSRTLPPRTSGLLDGQD
                     SLTVPTIRAKAAAQIAGLGAVYLPMLWAAPHIAAGRLVKKDIEGSKALTHVYLAWHPR
                     QAGKAGKWFIDRLRDKSVQQYVLGESGSYPAATG"
     gene            7428..8627
                     /locus_tag="VN496_10495"
     CDS             7428..8627
                     /locus_tag="VN496_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF17425.1"
                     /translation="MHTPTTRNVTILTVLVTVALLAGCGSKSDATAAAQKSEPPKTDS
                     AKPADGKPASPPPAAAAAKPGLPVKAEPVQIIRVSDDVSAVGSLLAEESVIIRPEIDG
                     RIVGLHFQEGQAVSAGARLVTIDSTEYEAQANAQRADLKTEEQRLVRTKELHEQKFIS
                     KDALDVQVGNVARLKAHVEEAESRVAKTVIRAPFSGILGLRQVSPGAYVKAGADIVRL
                     ENVSSIKVDFRIPENYLSKIRPNQEIAVKLDAYPGEEFKGRVYAVEPVVDERTRTIAM
                     RARIPNKGTKLKPGMFVRVAVTLDSRQNAVVIPEQAIWPQGKDSFVYRVVDGKAALTK
                     VAIGNRAPGSVEILKGLAANDMVITDGQMKLKDGAPVSVIALPPSVPPVVPGAAPAAP
                     GAPAAKS"
     gene            8661..>9685
                     /locus_tag="VN496_10500"
     CDS             8661..>9685
                     /locus_tag="VN496_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF17426.1"
                     /translation="MILSEISIKRPVLATVMSLVIVLMGFLSYSRMAVREYPNIDPPI
                     VSVRTVYKGATAQVIESAVTQPLEDSLSGIEGIKTIKSNSREEVSQITITFLTTREVD
                     AAAADVRDRVARIRKQLPATIDEPVVSKIEADAQAIIWIAVTSDRQTPMEITDFADRY
                     LTDPLKALPGVSSVIIGGERKYAMRIWLDRERLAAQGLTVQDVENALNNQNVESPGGR
                     LESTQREFTVQPRTDLRSPEDFNNMIISTAGYPIRLKDVGRAIPGPYENRKIVRVSGN
                     EAIGLGIVKQSTANTLEVARGVKEVLPRLQRTLPPGMKAWIAVDTSVFIEESIKAVYE
                     TMIEALIL"
BASE COUNT         1874 a         2888 c         3014 g         1909 t
ORIGIN      
        1 gaagcccgag caccacagcc gtaggcttcc cgaggccaac gggccatggt cgctgggtct
       61 cgaactggaa attcctcttg tggcgcaagg caagcgcgcg gctcaggctg agcgcggcgc
      121 cttccttgcc gatgcggcgg atctcgacct tgcccatgcc gtctggctta cccgcagtcg
      181 cgtccgcgat cagtacctcg acctgcgtgg aagtcgcgag gcgctcgtct cgctggatgc
      241 gcaactcgcg gcgcgccagg agatgatcgg cctcgtgaat cgccgggttg aagcgggcat
      301 gctgtccgcg cgcgagctag cggccgaacg ggtcgcttat tcgcagatcg agcaatcgcg
      361 caaccaggaa ctgacgaagc agcaacgtgc aaacgctgcc ctcgcggccg cccttggcat
      421 gccaatggaa gtgctgacta gcatggcgtt gcgcttcgat gcacccctgc ccgagccgtt
      481 ggagctggat gcgggcacgc tgcgcggcat ggcgttgcgc aatcgcctgg atgtgcatcg
      541 caagctgctg gaatttggcg ccgcggacgc ggatgtcaag ttcgcggtgg cagcacaaaa
      601 tccggacatc accttcgggc ctggctatat gtgggatcag ggtgacaaca tctggtcgct
      661 cgcggtcggc atctcccttc cgccggcggc ccgcacgcgc gccatgattc gtgaaacgca
      721 agcccggcgg gaactcgcag cagagcaatt cgcggccgtt caattgcagg ttattggtct
      781 ggtcgcgagt accggcgcgc agtaccgtct cgcacgcgag cgggcgagcg ctgcggagcg
      841 ccagctgcaa acgcagcgtg aacaggagtt ccgcattgaa cggcaattcg cggccggatc
      901 agccgatcgg atgcaacgcc ttgcagcgcg tattgaaacc attaccgcgg aagccgtcct
      961 gcagtcggcg caggtggctc agcgccaggc gctggcgcaa atggaagacg ccgtacagcg
     1021 tccgctactg ggcgatttgg cgctgccgcc tgtgccggga ccgcgcacgt cggacatgga
     1081 taatggaatg gcgaaccata gccgtcacca ggacaggcct caggcttccc ggttatgaag
     1141 gggcgtttgg catacgcgat catcgcgctg atgggattgg tgatcgctgc gctatcctgg
     1201 gcattggtgt actactcacg cgacgaactc ggtacgcaga cggaaaaacg cgatgatcgg
     1261 gtcacgtccg cgtcggtcgc tggcattgag tcggggcgag caatcgtgcg tttgtcggct
     1321 cagagccagg ccgccgctgg catcgtggtg caaccgttga agtcggcgac gcatgaatcc
     1381 gcgttggagg catacggcat tgtggcgaat ttgcagccgc tggcggagtg gcgcggccgc
     1441 tatctgtccg ccgcggccga ggccagggca gcgcgtgcga ctctggctgc cgcggaatcg
     1501 gaataccggc gcatgcaact gctgtttcaa gatgaccgca atgtctctga gcaggccatg
     1561 caagctgcgc tggcgcgcta tcgcgccgag cagttgcgcg tgagcgcggc cgatcaggcc
     1621 gctgcaagca tccgcgacgg gctccgcggc gcatggggag aaagcgtaac gcagtgggct
     1681 gtcaatcccg actcgcagac tatgcggtcg ttacttcaac agacatcgtt tctggtgcag
     1741 cttgttgtcc cgaaccagct accccgtggg gcgctgcgca accggatagc gattgcaccg
     1801 gcaatcgcgc gcgaaaatgt gcggcgagcc acctttgttg gcgcgtcccc gcagatcgat
     1861 gcggcatttc cgggggaaac ttatttttac atcgtaaatg gcggcggcct gcgcgccggc
     1921 atgcgtgtcg tggcacgctt cggcatgggc ggttcagcga ccggtgtgat tgtgccttcg
     1981 gcggccgtgg tctggcatgc aggcaaggcg tgggcatacg tcaaagatga cgacgatagt
     2041 ttcgcgcgct tcgaggttct gacagcagat gaaatggagg gtggctggtt caacaccgca
     2101 ggcttcgatg acgatgatga agtcgtagtc agtggtgcgc agttgctgct ttctgaagaa
     2161 ctcaaatatc aaattcgcaa tgaaaatgaa gactgacccg tccattgcgc gcagagtccg
     2221 tcccagaaag ctagaccttg ctgggatcag accggcatga gcggtgttgc ttcaggggca
     2281 ggtcgctgtt gatttccaca atcgtccgcc agtccattcg ctattccggc gtggtcgttg
     2341 cgctagcgct gacgatcgtg gtttacggct tgtttacgtt atcgcgcgcg catctggacg
     2401 tcttccccga gttctcgcca agccaggtcg tcattcagac cgaggccccg gggctttccg
     2461 ccgaactggt ggaaaagcag gtctcccagc ccattgaaaa tgcactttcc ggcgtatccg
     2521 ggctcgataa tctgcgctcg caatcaattc cggggctgtc cgtcctgcat gtttttttcc
     2581 aggaaggctc ggacatctat cgcaaccgtc aggtcgtcag cgagcgtctc gggtcggtgg
     2641 ttgcgcagct gcccaaggga atcgtcccgg tcatgacgcc gctgacgtcc tcggccagca
     2701 cggttctggg tctgggcgtc acgtcgtcgc agcgcagcct catggaattg cgcacgctgg
     2761 tcgactggac cattcgtccg catttgctgg cagtgcgcgg cgtggccgaa gtcaatgtat
     2821 tcggtggaga cgtgcgccag tggcagatcc aggtcgaccc cgaaaaaatg catcgcttcg
     2881 ggatggcgct ggacgacatc gtgttcgccg cgcgccgggc aaccggtgtc gcgggcgctg
     2941 gattcgtcaa aacgccaaac cagcacctcc ttctgcaagt cgatgtgcaa cccgatgcgg
     3001 tgacggatgt cgcgcacgcc gtacttgcct accgagatgg ccgtgccgtg cgcatcggcg
     3061 atgtcgctga cgtaacggaa ggcgcagctc cctccatcgg cgcggccgcg atagacggta
     3121 cgccgggcgt gttcatgctg gttcagggcc agcttggttc gaacacgcat gcggtcacgc
     3181 aggacctcga aaccgcagtg gccgaaatca ggccattgct tgaaaaagat gggatcaagc
     3241 tctatccacg gctgttccgt ccggctaatt tcatcgagac ggcagtgcgc aatgtccagc
     3301 gtgacattct tatcggttcg ctgctggtag tggtcgtgct gttcctgttc ctgttcaata
     3361 cgcgtacggc tttcatctgc gccacggcga tcccggtttc cctgctggcc gcggtactgg
     3421 tcctcgattt ttttaacgtc acattcaaca tcatggtaat gggcgggctc gcgattgcgc
     3481 tgggcgaggt tgtggacgac gcaatcattg ataccgagaa tatttttcgc cggctgcgtc
     3541 ttaatcgcag tttgtcgacc ccccgaccgg tgtcggaagt ggtactggcg gcatccattg
     3601 aagtgcgcag ttcagtggtc tatgcgacct tcattgtcgc tctcgtcttc gtgccgctgc
     3661 tgaccttggg cggcgtggcg ggcaaactgt ttgcgccact cggtttggcg tacattttcg
     3721 caattctcgc gtcactgctg attgccgtga cgctgacgcc ggcgttatgt tacctgctgc
     3781 ttgcgggcgc ggaactcaag gcggacgatc cgccgttggt tcgctggatg aagccaaagt
     3841 atttatttgt actcgggcgc attgagcgtt ttcccgggcg tgtaatcgct gttaccgcta
     3901 tcgtcatcgc aatcggcatc gcatttttgc cgctgttcgc agggcagttt attccggcct
     3961 tgcgcgaagg ccattacatc ctgcacatgt ccgcggtgcc cggcacatcg gaagcggaat
     4021 cgctgcgcat cgggcgccgg gtgtcgcagg caatcggatc catcaaggga gtgcagtcgg
     4081 ttgcgcaatg ggtcggccgt gccaagaacg gcgctgatac gttcggtacg cactatagcg
     4141 agttcgaagt cgagatcggg ccgttgcccg gcaaggaaca agtgcgcatc ctggaagaga
     4201 ttcgccaaac gctttccggc aaacgcggcg gaggctttcc gggcgtgaca tttgccgtca
     4261 atactttcct gaccgagcgt atcgaagaaa ccatcaccgg ctacgcggcg cccttcgtcg
     4321 taaacctgta cgggtcgtcc ctggacctgc ttgaccgcga cgcgctggcg ctggcctcgg
     4381 tgctcgccgg agtgcccgga gcgcgtgatg tgcagattca ggcgcagccc ggcgcgccgc
     4441 aattatcgat acggttgcgc cacgaacgtg ttgcggcgct cggactgcag ccgcttgccg
     4501 tgctggagac ggttcagacg gcatatgaag gcacacaggc ggcacagatt ttccggggca
     4561 atcaggtcat cgatctggtc gtagttctcg atcccgcgca ccgcgatcgc atccacgaca
     4621 ttgccgcgct gccattgcgc aacccggacg gcatgctggt ccggctcggc gatattgccg
     4681 acatccagat catcagcggg cgctacaaaa tcctgcatga ttcgggacgg cgcctccaaa
     4741 ccgttacggc agacgtcgtg ggccgcgaca tcgaacagtt cgagcgcgat gcccgtgccc
     4801 gcatagctgc ggcaggcaca ctctcagagg gcaattacgc tgtattcgcc ggcacggcgc
     4861 aggcgcaggt acaggcccgt tccgacctgc tgctgcacgc cgcgctcgcc gcgatcggca
     4921 tctttctgct gctctacatc gccttcaata atctgcacaa cctgctaatc acgtccatca
     4981 acctgccgtt cgcgctgatc ggcggagtaa tcgccgtggt ggccagcggc ggctggatgt
     5041 cgctcggctc gctggtcggc tttgtgacgc tgttcggcat cacgctgcgc aactcgatca
     5101 tgctggtatc gcactatcag catctggtgc aagttgaacg gatgccgtgg aacgccgata
     5161 ccgctttgcg tggcgccgcc gagcgcctgc cttcaatcct gatgactgcg ctggtcaccg
     5221 cgctggggct gatgccactg gcggtgggta gcggcgaacc gggacgcgaa atcgagggac
     5281 ccatggccac cattattgtg ggtgggctgg caacttcgac gctgttgaat ttgctggtgc
     5341 ttccagccat cctgctgcac tttggccggt tcaagtcgag tgcggcgggg cgggcttgat
     5401 ctgcaggaag cagcctccac ataccggcga tacgccaaca gcttgaagcg cgcatgggca
     5461 acaaggagag cccagtcatg tcgacaattg cactgaccaa ggacaatttt gaacaagtgg
     5521 tgaccggcaa cgatacggtt atcgtggatt tttgggcgcc gtggtgcggc ccctgccgca
     5581 gcttcgcccc ggtcttcgaa gcgacttccg agaagcacga aggggtggtg ttcgccaagg
     5641 tcaataccga ggaagaaccg gagctagccg ggcacttcga aattcgttcc attccgacgc
     5701 tgatggtgtt ccgcgaaaaa gtgctgttgt ttgcacagcc gggcgcacta ccggcttcag
     5761 ggctggaggc ggtgatccag aaagtgaaag ccgtcgacat ggcggaggtg catcgcgaca
     5821 tcgcgacgca ggaagccgcc gcaggggccg aaaaggccaa taccccgagc tgaaaaacgc
     5881 gtatggcgcc agcgttcctc gacgaaacca gttttgacag cttcgtgtcc gctcatgaca
     5941 cagcagtaat tggattcatc caacagaaca ctgacgcagc aaaattttcg gcgcttgcag
     6001 atgcagtagc ggccaaatat cccggcgttg cgtttgcaca ggtcggggcc agcagccgaa
     6061 cgctgttcga gacgttcggc atcggccgga cggctaccgc gatttttcgc gaacgcatcg
     6121 gcatgtattt ggagcctggg ttcccggcgg ccgaactgct ggaccgcatg ctcctttcga
     6181 tcacggcgct ggacatggaa catgttcgcg gcgaactcga gcaggagaaa gccgcgcggg
     6241 aatccttggc cgtgcatcgc gtatgtccgg ctacgcggcg tggcaagctc tgaatgtatt
     6301 cagcccgtcg ctgccggata actgcccgat tcgccaagca cgtattgctg aactgatttg
     6361 tcgcgcagcc ggtcgatgaa ccatttgccg gccttcccgg cctgccgggg atgccaggcg
     6421 agatagacgt gagtcagcgc cttcgagcct tcgatatcct ttttcaccaa ccgccctgcg
     6481 gcgatatgcg gggctgccca cagcatcggc agataaacgg cgcccagtcc ggcaatctgc
     6541 gcggccgcct tagcacgaat ggtcggcaca gtaagcgagt cctgaccatc cagtaatccg
     6601 gatgtgcgcg gcggcagcgt acgagaggta tctgcggccg aaatggcccg atgttgccgg
     6661 atctcctgcg cggcaagtgg ctccttggca gtggcgagtg gatggtctgg cgagaccacg
     6721 aagacaaact caaagctgcc tatgggttcg accgccagca ggccttccgg cggcgcatcc
     6781 cccggcgccc caatcaccat gtcggcgcga ccgccaatga gggcgtccca gcaaccgccc
     6841 aacacttcag tactcaaccg gatgcgcgtg ccggattgct cgcggtaaaa atccttcaga
     6901 agcggaaaca ggcgctcgat tggcaccaag tcatccacgg cgatgcgcaa ctcgctttcc
     6961 catccggtgg caacgtgttt cacgcgacat tccagatcgg ttgcagtacg caataagtgg
     7021 cgcccttccc gaagcagggc ctcgcccgct tcggtcaggc gcgcccggtg cccgctgcga
     7081 tcgaacaact ggacgtcgag atcctgctcc agcttctgta tcgtgtaggt aatagccgag
     7141 ggcacgcggt gcaattcttc cccggcagca gcaaaactgc cgttacgggc aatggaatcc
     7201 agaaccagca atgcgtccag tgaaattctc atggtggccg ctgaaaaact cggttatcat
     7261 taaaaaataa ctgactagca agtcagcaaa tgctgtcgaa cgggtccgca cattgacaaa
     7321 cctctccgac ggtctactgc cagccgagat attccgctgg ctaaaatttt ttcacttgtc
     7381 gtagcggctg ggtgcgggcc gtcgaccttg aattctaaag ggatgccgtg catacgccga
     7441 ccactcgtaa tgtaacgatt ctcacggttc tggtcacggt ggcgctgctt gccggttgcg
     7501 gctcgaagag cgacgcgacc gctgcggcac agaaatcgga accgcccaag actgattcag
     7561 ccaagcccgc ggacggcaag ccagcttcgc cgcccccggc cgcagccgcc gccaaaccgg
     7621 gattgcccgt caaggccgag ccggtgcaga tcatacgggt ctccgacgat gtttccgcgg
     7681 tcggttcact gctggcagag gagtcggtga ttatccggcc cgagatcgat ggccgtatcg
     7741 tcggtttgca tttccaggaa ggtcaggccg ttagtgcggg tgcccgactt gtgaccatcg
     7801 atagcaccga gtacgaagca caggccaacg cccagcgtgc cgacctgaag accgaggaac
     7861 agcgcctggt acgcaccaag gaactgcacg aacaaaagtt catcagcaag gacgcgctgg
     7921 atgttcaggt cggtaacgtg gcgcgcctga aagcccatgt cgaagaggcc gagtctcgcg
     7981 tggcaaagac cgtaatccgg gcaccttttt ccggcatcct gggcttgcgc caggtcagtc
     8041 ctggcgctta cgtcaaagcg ggcgccgata tcgtgcgtct tgagaatgtc agctccatca
     8101 aggtcgactt ccgcattccc gaaaattacc tttccaagat tcgtcccaat caggaaatcg
     8161 cggtgaaact cgatgcctac cccggagagg aattcaaggg gcgtgtttac gcggttgagc
     8221 cggtggtgga cgagcgcacg cgcaccatcg ccatgcgcgc acgcattccg aacaaaggca
     8281 ccaagctcaa gccgggcatg tttgttcgcg tggccgttac gctggatagc cgccaaaacg
     8341 cggtggtgat tcccgagcag gcgatctggc cgcagggcaa agacagcttt gtgtaccgcg
     8401 tggtggacgg caaggccgcg ctgaccaagg ttgccatcgg caatcgcgca ccggggtcgg
     8461 tggaaattct gaaaggcctg gcagccaacg acatggtcat taccgacggc cagatgaagc
     8521 tgaaggacgg cgctccggta tccgtgattg ccttgccgcc gagtgtcccc cccgtggtcc
     8581 ctggcgcagc gccggccgct cccggcgcgc cggcagccaa gagctgagcg tcacgcaata
     8641 attcgcttag gagcgcagcg atgattcttt ccgagatttc gatcaagcgg ccggttctgg
     8701 cgacggtcat gagcctggtc atcgtgctca tgggcttcct gtcctattcg cgcatggccg
     8761 tgcgtgaata tcccaatatc gatcctccga tcgtttccgt gcgcaccgtg tacaagggcg
     8821 cgacggcgca ggttatcgaa agcgcggtca cgcagccctt ggaggattcg ctgtccggca
     8881 tcgaaggcat caagaccatc aagtccaaca gccgcgaaga agtcagccag attacgatta
     8941 cgttcctgac tacgcgggaa gtagacgcgg ctgcggcaga tgttcgtgac cgtgtcgcgc
     9001 ggattcgcaa gcagcttccg gcgacgattg atgagccggt tgtatcgaag atcgaagccg
     9061 atgcgcaagc catcatctgg atcgcagtca ccagcgatcg ccaaactccg atggagatta
     9121 ccgatttcgc ggatcgctac ctgaccgatc cgctcaaggc gctgcccggc gtgtcgtccg
     9181 tcatcatcgg gggcgagcga aaatacgcga tgcgcatctg gctcgaccgc gagcgcctcg
     9241 cagcgcaggg gttgaccgtc caggacgtcg agaacgcgct caacaaccag aacgttgaat
     9301 caccgggcgg acgcctcgag agcacgcagc gcgagtttac cgtccagccg cgcaccgatc
     9361 tgcgctcgcc cgaagatttc aacaacatga ttatctcgac ggcgggctat ccgatccgcc
     9421 tgaaggatgt gggtcgcgca atccctgggc cttatgagaa ccgcaagatc gtgcgggtga
     9481 gcggaaacga ggcgatcgga ctcggtatcg tcaagcaatc gactgcaaac acactcgaag
     9541 tcgcgcgcgg ggtgaaggaa gtgctgccgc gcctgcaaag aaccttgccc cccggcatga
     9601 aagcctggat cgcagtcgac acatcggtct tcatcgagga atcaatcaag gcggtgtacg
     9661 aaaccatgat cgaagccctg atcct
//
LOCUS       DATMZQ010000330         2879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4970385, whole genome shotgun sequence.
ACCESSION   DATMZQ010000330 DATMZQ010000000
VERSION     DATMZQ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4970385"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..2053
                     /locus_tag="VN496_10505"
     CDS             311..2053
                     /locus_tag="VN496_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF17427.1"
                     /translation="MRLGPFERSPRDRRKTDTLQEGEARFRALVQNSFDIITIHDSNG
                     VTLYESPAASRVLGYPPGTLIGKTPFETLHPKDVGRAREAFEALIKGEVAVAPVVLRF
                     RHADGSWIHLEALGNNLLDHPGIRGIVLTSRDVTERTRAEERIQYLANYDVLTGLPNR
                     SLMQDRLTQVIAHAHRNRLRVALVHIDLDRFKVVNETLGHYVGDVLLKQAAERMRKST
                     NGADTVARVGGDEFTIIFPNVTSLQALSASAEIMLDDLAKPFPGDGQELFVSASVGIS
                     LYPDDAGSVDELIKHADAAMSTAKQLGRNNFQFFTPGMNQEAQDRMLIEGGLRNAIQR
                     EELGLLFQPKIDLATRQMFGAEALLRWKHPKLGMISPSRFVPVAEESGLVGQIGEWVL
                     HTACRQIREWQDAGYSLQVAVNVSARQFQEYDVAELVKDILRDTGVLAQNLEIELTES
                     AVMNDAESSIVTLERLAALGVQIAIDDFGTGYSSLSYLKRLPLDVLKIDQSFVRDISS
                     DPNDAAIVRAIITLARSLGIKVIAEGVENEAQLAFLNAYGCHYAQGYLFGKPLTAAQL
                     GKLIATGALEHTIA"
     gene            2318..>2879
                     /locus_tag="VN496_10510"
     CDS             2318..>2879
                     /locus_tag="VN496_10510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXF17428.1"
                     /translation="MAEKILAFAGSLRSGSFNRKLIRIAAEAARERGAIVDEIHLGDI
                     PMPLYDADVEREHGLPANAKLFKQLLVEHRGVLIGSPEYNTGMSAVLKNAIDWASRPE
                     PGEPSYLAFKGKVGGIMSTSPGNFGGVRSLGTLRGILSYLGMIVVPTQLAIPRANAAF
                     DPEGKLTDETYAKTVRSIGDEVVAFLR"
BASE COUNT          607 a          810 c          865 g          597 t
ORIGIN      
        1 gcttggccgt gctgctcgac ggggtggtca aggaatacga gaaccgcttc gggacactta
       61 atatcgatgc aactccgccg ccggccaaat aggggggtga ccggagacct cgatcggaaa
      121 cttagagaga acggcttcgt cgcactggaa attgttcttt aaatgcagta taaacaagcc
      181 acaataataa gcagcttaca ataaagttta gatagtccgg gcaatcacac atgtccggtt
      241 tgcagggggt gatcgtgaaa ttttgcaaaa ctagcgtccg ccggcacaag ttgcctgggc
      301 ggggatagtc atgcggctgg ggccattcga gcgttcgccc cgcgatcgcc gcaagacgga
      361 caccctgcag gagggcgagg cgcgctttcg cgcgctggtg caaaactcgt tcgacatcat
      421 caccattcat gactccaacg gggtgactct gtacgagtcg ccagcggcgt cacgcgtgct
      481 gggctacccc ccaggaacac tgattggcaa gactccattt gaaacactgc acccaaaaga
      541 tgtcggtcgt gcgcgcgaag cctttgaagc gctgatcaag ggcgaggtgg cggtagcgcc
      601 ggtggttctt cggtttcggc atgcggacgg ctcgtggatc catctcgagg ctctgggaaa
      661 caacctgctg gatcatccag gcatccgcgg catcgtgctt acctctcgcg acgtgaccga
      721 gcggacgcgc gcggaagagc ggattcaata tctcgccaac tacgatgtgc tcaccgggtt
      781 gccaaaccgg tcgctgatgc aagaccgcct gacccaagtc atcgcacatg cgcatcgcaa
      841 tcgcctgcgt gtggcgctcg tgcacatcga cctggaccgg ttcaaggtgg tgaacgaaac
      901 gctgggccat tatgtcggcg acgtattgct gaagcaggcc gccgaacgta tgcgcaagtc
      961 gaccaatggg gcggataccg tcgcccgggt agggggagac gaattcacaa tcatatttcc
     1021 gaatgtgacc agtctgcagg ccttgtcggc ttccgcggaa atcatgctgg atgaccttgc
     1081 caagccgttt cccggcgacg gccaagaact gttcgtttcc gcgtcggttg gcatcagtct
     1141 ctatccggat gacgccggca gcgtcgatga actcatcaaa cacgcggacg ccgcaatgtc
     1201 cactgcgaag caactggggc gtaataattt ccagttcttt accccgggca tgaaccagga
     1261 agcccaggat cgcatgctga tcgagggagg gctgcgcaac gccatccagc gtgaagaact
     1321 gggcttgctc ttccagccca aaatcgatct ggccacgcgc caaatgtttg gagcggaggc
     1381 cttgctgcgc tggaagcatc ccaagctagg catgatttcg ccgtcgcgtt tcgtgccagt
     1441 ggccgaggaa tcgggactgg tgggtcagat cggggaatgg gtattgcata cggcctgccg
     1501 tcagatacgc gagtggcagg atgccggtta ttccttgcaa gttgccgtca atgtctcggc
     1561 acgccagttc caggaatacg atgtcgctga gctggtcaag gatatcctgc gcgataccgg
     1621 cgtgctggcg caaaacctcg aaatcgaatt aaccgaaagc gcggtcatga acgatgcgga
     1681 atcttcgatc gtgacgctcg aacggctggc tgcgcttggg gttcagatcg caatcgacga
     1741 tttcggcacc ggttattcga gcctgtccta tctgaagcgg ctgccactcg atgtgctgaa
     1801 gattgaccaa tctttcgtac gcgatatttc gtcggacccc aatgatgccg ccattgtgcg
     1861 ggcgatcatc acgctggctc gcagcctcgg catcaaagtc atcgccgaag gcgtggagaa
     1921 cgaggcgcaa ctcgcgtttc tgaatgcgta tggctgccac tatgcgcaag gatatttgtt
     1981 cggcaaaccg ctgaccgcgg cgcaactggg caagctgatc gctaccggcg cactcgaaca
     2041 taccatcgct taaggcggca caccgctctt ggcagcacgg agcaaccgga tgtcatcggg
     2101 cgcgttccgg gtttatctca atcttttgtc atagcgtgct ctgctagaat cgcttgtgct
     2161 tggggcccgc gtggcggcat gccagcagtt atgccatttt cgcttccagg cgcctttcca
     2221 aggtcttcga tagttaatca tgcaggatgc cgagcggcgc ctgcccatgc tgcctggcga
     2281 aggtccgatt ccgacgtaac ctgaagggcc acgcttaatg gcagaaaaaa ttcttgcgtt
     2341 tgcgggcagc ttgcgcagcg gatcgttcaa tcgcaagctg attcgcatcg cggcagaagc
     2401 cgcgcgcgag cgcggcgcga ttgtcgacga aatccatctg ggagacattc caatgccgct
     2461 ttacgatgcg gacgtcgagc gcgagcacgg gctgccggca aatgcaaagc tgttcaaaca
     2521 gctgctggtc gagcatcgcg gcgttttgat tggttcgccg gaatacaaca ccgggatgag
     2581 tgcggtgctg aagaatgcca tcgattgggc ctcccgcccc gaacccggcg aaccctccta
     2641 tcttgcgttc aaaggcaaag tgggcggcat tatgtcgacc tcacctggaa atttcggtgg
     2701 cgtgcggagt ctcggcacgc tgcgcgggat attgtcctat ctcggcatga tcgtcgtgcc
     2761 gacgcaactg gctatcccgc gggccaacgc cgcgtttgat ccggagggta agctcaccga
     2821 tgagacctac gcaaagaccg tacggtccat tggtgacgaa gtcgttgcgt tcctgcgta
//
LOCUS       DATMZQ010000331         7215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3034485, whole genome shotgun sequence.
ACCESSION   DATMZQ010000331 DATMZQ010000000
VERSION     DATMZQ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034485"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1188)
                     /locus_tag="VN496_10515"
     CDS             complement(202..1188)
                     /locus_tag="VN496_10515"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17429.1"
                     /translation="MKSRVAAWLLVVSSVAIGWTGQSWAEQKTARVGILTFFAFKDDP
                     TLNFWFKPFRRALVEQGWTEGKNLSFEYRSAQSDPSKFEAAASELARLNVDIILAVGA
                     PAVHAAYAATRSIPIVANDFTNDPVAEGYVKSYGKPDGNLTGTFLDAPDFAGKWFELL
                     KVIVPKLSRVCVLWDPSPGNNHLQAVRSVANSLAVKLKIIEVRTPEDIGRAFTSLRGC
                     PQAMIILPSPLTYVQSARLAELAAKHRLPATSMARAFADAGGVVAYGPDLVSTFQSNA
                     AMVDKILRGAKPGQLPVERPTKFHLVINLKAAKILGITIPATVLQQADEVIQ"
     gene            complement(1236..2219)
                     /locus_tag="VN496_10520"
     CDS             complement(1236..2219)
                     /locus_tag="VN496_10520"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17430.1"
                     /translation="MKKQVILLLAVGLTMMGWWSGAGWAQAKLPRIGILTYAGTTDAA
                     AKQWLEPFWRKLAELGWIEGRQVLFEVRRAGGDSSLVAEAARELVRLKVDVIFADSAP
                     SVRAAHAASTTIPIVGADFTNDPVAEGYVENYGRPGKNITGVFLDAPDFAGKWLELLQ
                     GIVPNLSRAVVLWDPSPGSAHLQSVKDIARSLRLQLQVIEVREPVDIDRAFSLLRGRP
                     QALIVLPSPTLFLQGERIAKLALEHRLPATSMSLPFAEEGGALAYGPEGDAAYGACAV
                     LVAKILAGTKPGDLPVERPTKIALVVNLKSAKALGLSVPQSILLRADKVIR"
     gene            complement(2261..3292)
                     /locus_tag="VN496_10525"
     CDS             complement(2261..3292)
                     /locus_tag="VN496_10525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17431.1"
                     /translation="MKQAFACNKHLRTREQWAAGVLAIAAALLTSFGWTIEAVGQGNV
                     KQVGILAVDVDVNGEVAKQWWKPFQRTLADQGWIEGKNVAFVFRKPSGSSPEFTDAAN
                     ELVRLKVDVIWAISTPAVRAAYAATRTVPIVALDFTTDPVSQGYIESYARPGGNLTGV
                     FLDAPEFAGKWVELLKAIVPGLSRVAVLWDPSPGPAHRSAVERATLSFGVKAQVIEVR
                     KPADIDRAFASMRGHPQALIILPSPLLYFQNNKVAALALKHRLLATSLFRLFVQEGGT
                     LAYGPDQAWASARDAVLVAKILNGSKAADLPVERPEKFQLVVNLKTARAIGITIPQSI
                     LVNADEVIR"
     gene            3553..4215
                     /locus_tag="VN496_10530"
     CDS             3553..4215
                     /locus_tag="VN496_10530"
                     /inference="COORDINATES: protein motif:HMM:NF021885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HXF17432.1"
                     /translation="MEDIEHPNRRDFLALATTALAAVSVAALPAASVSAATTSGTDFT
                     RWLDSIGGKHKVVLDMREPNGGMALAWAWVFLLTGQQAYAVPESDLGVALVLRHNGIP
                     IAFNDSAWKKYKLGEVFKIDDPETGAPAVRNPYYLKMNEPFLPDMAMQKLIDRGVRVI
                     ACDMAIHFYSGIVAKQTGLKHEDIKADWNSAVLPGIAHAPSGVVACQGAVARGCTYLF
                     AG"
     gene            4329..5144
                     /locus_tag="VN496_10535"
     CDS             4329..5144
                     /locus_tag="VN496_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF17433.1"
                     /translation="MKKPCIALVCSLVLLGCSAAAVARPTIDELKARAVLDDYESNVV
                     FGYNIVMETNRYAGRYAGNALNCTSCHLKGGTQPDGFPLNVGGVYPKWRSKNGIRNGL
                     GLRIRDCFLYSLNGVMPPEESAEVQAVAAYINYLSEGEVIGRPPKGSGVPTLPDTGAD
                     PNPPRGQTVYIRQCAVCHGVNGEGNGAIPPTWGLRSYNAGAGLGNVQRLAGFIWANMP
                     KGKERSLTHQEALDVAAFINLQHRPFDPREGSLKKLVEVIYQRLSGFTGKRNP"
     gene            5170..6669
                     /locus_tag="VN496_10540"
     CDS             5170..6669
                     /locus_tag="VN496_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXF17434.1"
                     /translation="MSLLREALPDFSWWRAVDTRREAIAGVTGAALVIPQAITFAYLV
                     GLAPEHGLYCAVFVGLLASLFGNSAMVGGPNTAVSILLGLTVLPFAGRGSPLFTELVL
                     VLSLMVGLIQLCIWLLRGAAVFRYFSPAAIVGIKVGVGVLLVTSALEGALGLSPMTTK
                     FFYEKFYIAIGSWQDLVNPYAAIISAITIGSGLLLKTSWPRSYIIGAVVIGSLVGGAL
                     YGWLGPVQSQLELLGHVTLHALPLNMPRLGPQHWLFVEQVVPQAAAIAVLGLSQSLVI
                     ARDLKATVAHGLNLHKEVFAQGIANVLSPFFSTFAGSGSFNRTSVAHEMGARTPLAGI
                     IAAFAVVLIAWTIGPLLTWLPMPVIAGVMALVGIGMVQGNQIAPLRNRIDGTVFLLTL
                     LVVVFVGLEYGILVAVLASVGFFVASASRVKLSISHDAGKQHIAVTGNLFYASLDSLS
                     DCLRADPTAYTVLDLSRVPYCDASATEMIETIQRDRLRHGGQLLVTAGA"
     gene            complement(6700..>7215)
                     /locus_tag="VN496_10545"
     CDS             complement(6700..>7215)
                     /locus_tag="VN496_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXF17435.1"
                     /translation="VMSVRLEEPAPEEPRPEPPKKQKPKPERKIKQTPVVVPKPLPNE
                     VQSKIVLPKVEPKPKPEPKPKEEEKPPEPVPEPKPEAQPLPTVFRDVKPVKKIKPKYP
                     PDAEAKHIEGRVRVRLTVEVNGSVSAARILAADPPGVFDAAVMEAVVQYIFKRDGTSY
                     EADQEIVFKLE"
BASE COUNT         1485 a         2087 c         2047 g         1596 t
ORIGIN      
        1 cagttcgcac tggtgcagaa acaattcgaa cgtactgaac gcatacaaga gcgtgcggag
       61 aaactgcagg acaagagcag ggaaatggtt ggggcggccc gcaagatcct ggtagtcatc
      121 gtgccgatcg ttgtcgcgct aatcgtatac gtgtcgtttt tgatcttcag acggtagcgc
      181 gcggtactca caacacaatg attattgaat tacctcatcg gcttgttgca agacggtcgc
      241 tggaatggtg atgccgagaa tcttcgcagc cttcagattg atcacgagat ggaacttggt
      301 aggccgctcc accggtaact ggcccggttt ggcaccgcgc aatatcttgt ctaccatcgc
      361 ggcattgctc tggaacgtgg aaactaagtc cgggccgtaa gccacaacgc cgcctgcatc
      421 ggcgaatgcc cgcgccatcg acgttgcggg caaacgatgc ttggcagcaa gctctgccag
      481 gcgcgcgctt tgtacatacg ttagcggcga aggcagaata atcattgcct gcgggcatcc
      541 ccgtaacgag gtgaaagccc tgccgatgtc ttccggcgtg cgcacctcga taatctttag
      601 ctttacagcc aaagagttcg cgacactgcg taccgcttgc aggtgattgt tgcccgggct
      661 gggatcccac aatacgcaga cgcgggacaa ttttgggaca attactttca atagctcaaa
      721 ccatttgccg gcaaagtcgg gcgcgtccag gaacgtccct gtcaggttcc catccggctt
      781 gccgtagctt ttaacatagc cttcagcaac cggatcgttc gtgaaatcat ttgcgacaat
      841 tggaatgcta cgagtagccg cgtaggcagc gtgaactgcg ggcgcaccga ccgcaaggat
      901 gatatctaca ttcaatcgtg caagttcact ggcggcagcc tcgaatttcg acggatcgct
      961 ttgcgcacta cgatattcga acgacaagtt cttgccttcg gtccaaccct gctcgaccag
     1021 cgcgcgacga aacggcttaa accagaaatt cagcgtcgga tcatccttga acgcaaaaaa
     1081 cgtgagaatg cccactcgcg cggtcttttg ctcggcccag ctttgtcccg tccatccgat
     1141 ggcaaccgac gagacaacca gcagccaggc ggcaacacga ctcttcatac cgtctcgggt
     1201 cggtgaaccg tgcttgcaaa gtgtcatggt catgattagc ggattacctt atcggctcgc
     1261 aacaggatcg actgcgggac ggacaacccc agcgccttgg cggacttgag gtttacgaca
     1321 agcgcaatct ttgtcggtcg ctccacggga agatccccgg gttttgtgcc agcaagaatc
     1381 ttggcgacaa gcacggcgca ggccccgtac gccgcgtccc cctcaggccc gtaagcgagt
     1441 gcgccaccct cttcggcaaa tggcagactc attgatgtag ctggaagccg gtgctcgaga
     1501 gccaatttcg cgattcgctc gccctgcagg aacagggtcg gcgaaggcag cacaatcagc
     1561 gcctggggcc gtccgcgtaa caaggaaaat gcccgatcga tgtccaccgg ctcccttacc
     1621 tcgatcactt ggagctgaag acgcaatgat cgggcgatat ccttcacgct ctgtaagtgc
     1681 gcagagccag ggctcggatc ccagaggaca actgcgcgtg acaagttggg gacgatcccc
     1741 tgcagcaatt caagccattt acctgcgaaa tctggcgcgt cgaggaatac accggttatg
     1801 ttttttcctg gccgaccata gttttcgaca taaccttcgg cgaccgggtc gtttgtgaaa
     1861 tcggcaccga cgattgggat cgtcgtgctt gcagcgtgag cggcacgcac agaaggtgcg
     1921 ctgtccgcaa aaatcacatc caccttcaat cgcacaagct ccctggccgc ttcggcaacc
     1981 agtgagctat ccccgcctgc acgccggact tcaaacaaga cttggcgacc ttcgatccat
     2041 ccaagctcgg caagcttgcg ccaaaacggc tccaaccatt gcttcgctgc cgcatcggtc
     2101 gtgcccgcgt aggttagaat tccgatgcgc ggaagtttcg cttgcgccca gccagcaccg
     2161 ctccaccatc ccatcatcgt caagccaact gcgaggagca ggatcacctg tttcttcatg
     2221 ctcttttggg ccagcttatg gaataaggga aagactttca tcaccgaatg acctcatcgg
     2281 cgttcacgag aattgattgt ggaatcgtta tgccaatcgc cctggcggtt ttgagattaa
     2341 caacgagctg gaacttctct ggccgctcca cgggcagatc agcagctttg cttccattga
     2401 ggatcttggc aacgagaaca gcatcgcgcg cgctcgccca cgcttggtca ggtccataag
     2461 ccagcgtgcc gccttcttga acgaagagac gaaacagcga tgtcgccagc aatcgatgct
     2521 tcagcgccaa cgctgcgact ttgttgttct gaaaatagag cagaggggac ggcaggatga
     2581 taagcgcctg tggatgcccc cgcatggaag cgaaagcccg gtcgatgtcc gccggcttgc
     2641 gcacctcaat aacctgggct ttcacaccaa aagacagggt tgcgcgctcg acggcgctcc
     2701 ggtgagcagg gcctggactc gggtcccaca acactgcgac tctggacagg ccgggaacaa
     2761 tagcctttag taattcaacc cactttccgg cgaattccgg agcgtcgaga aagacaccgg
     2821 ttagatttcc gccaggccgt gcgtagctct cgatgtagcc ttgactaacc gggtcggtcg
     2881 taaaatccaa cgcgacgatc gggacagtgc gtgtggcagc ataagcagca cgtacggcgg
     2941 gcgtacttat cgcccagatt acgtcgacct tgagacgaac cagttcattt gctgcgtcag
     3001 taaactcagg agaactgcct gacggtttac ggaatacaaa cgcgacattc ttgccttcga
     3061 tccagccctg gtcggcaagt gtccgttgga atggcttcca ccactgtttt gcaacctctc
     3121 catttacatc tacgtcaacg gcaagaatgc caacctgctt tacatttccc tgacccacag
     3181 cttcaatcgt ccagccgaag ctcgttagca aggctgctgc gatggcgagc acgccagccg
     3241 cccattgctc tctcgtgcgc aggtgcttat tgcaggcgaa tgcttgcttc atcgggtcac
     3301 ctctctcctg acccgtaatg atgcctttat tatgcaaatc tgcacgataa ctttatccgg
     3361 atgggcgcga atcccactat ttcacaattt gtggggaagt tgtatgcgca ttgaatagct
     3421 gcgtgtttgt tcttggtgtt aggataacct gcagcaggga ttgaagttca tgggatggca
     3481 tccttgcctg caaacatccg caccgctcag aggtcttttg attcactcgt ttactcaccc
     3541 aaggagactg agatggaaga catcgaacac ccaaatcgcc gagatttcct cgcgcttgcg
     3601 accaccgccc ttgcagccgt cagtgtggcc gcattgcccg cggcaagtgt gagcgctgcc
     3661 acgacatctg gcaccgactt tacgcgctgg ctcgacagca tcggcggaaa acataaggtt
     3721 gtgctggaca tgcgagagcc aaacggcggc atggcgttgg catgggcctg ggtatttctc
     3781 ctgactggcc agcaggctta tgccgtacca gagagcgatc tgggggtcgc cctcgtgctg
     3841 cggcacaatg gcataccgat tgcattcaac gactccgcgt ggaaaaaata caaacttggc
     3901 gaggtattca agatagatga tccggagacg ggtgcgcccg ctgtgcgtaa tccctattac
     3961 ctgaaaatga acgagccttt cctcccagac atggcaatgc aaaagttgat cgaccgcggt
     4021 gtgagggtaa tcgcgtgcga tatggcgatt cacttctaca gcggtattgt tgcgaagcag
     4081 acaggtttga agcacgagga catcaaggct gactggaatt cagccgtgct cccaggtatc
     4141 gcgcatgcgc cttctggcgt agtggcgtgc cagggcgcgg tagcacgagg ctgcacctac
     4201 cttttcgccg ggtagaattt gcacgaagat gcgccgtagc gagcacgacg ccgggtaaag
     4261 gcatcgtgct ccttggttca tccggcgcag gccatctacg gcgggtgtgg ttgcatcgaa
     4321 caagcccaat gaaaaaaccc tgtatcgcac tcgtttgcag cttggtcctg cttggttgtt
     4381 cggcggcagc cgtcgcgcga cccacaatcg atgagttgaa ggcgcgtgcc gtactggatg
     4441 actatgagag caacgtcgta ttcggctaca acatcgtgat ggagaccaac cggtacgcgg
     4501 ggcgttacgc cggtaacgcg ctgaactgca ccagttgcca tctcaaaggt ggcacacagc
     4561 cggacggatt tccgttgaac gtcggcgggg tctatccgaa atggagatcg aagaacggca
     4621 tacgcaacgg gcttgggctc cgcatccgcg actgctttct gtacagcctg aacggcgtaa
     4681 tgccgccgga ggaatccgcc gaggttcagg ctgtcgcggc gtacataaac tacctgtctg
     4741 agggcgaagt gatcggccgc ccaccgaagg gctcgggtgt tccgaccttg ccggataccg
     4801 gcgcggatcc gaatccgccg cgcggacaaa cggtgtacat ccgccaatgc gcggtctgcc
     4861 acggggtcaa tggcgaaggc aacggcgcaa tcccgccaac gtgggggctg aggtcctaca
     4921 acgcgggcgc gggattaggt aatgtccaac gccttgctgg attcatttgg gccaacatgc
     4981 caaagggcaa agagcgcagc ctcacccatc aagaggcgct cgatgtggcg gcatttatca
     5041 acctccagca ccggccattc gatccacgcg aaggctctct gaagaaactg gtggaagtca
     5101 tttatcaacg cctgtccggt tttaccggga agcgcaaccc gtgagaccac gggcatcacc
     5161 gggcgcgcaa tgagcctgct gcgcgaagca ctgccggatt tttcctggtg gcgcgcggtc
     5221 gatacgcgtc gcgaagcgat agcgggagtc actggcgcgg cactggttat tccgcaagca
     5281 attacgttcg cttacctggt tggtctcgcg cctgagcatg gtctgtactg cgctgtgttt
     5341 gtgggattac tggccagtct gtttggcaat tccgcgatgg tcggtggccc gaataccgct
     5401 gtttcaattc tgcttggtct gacggtgctg ccattcgccg ggcgcggcag cccgctgttc
     5461 acggaactcg tcctggtgct gagcctcatg gttggactga ttcagctttg tatctggctg
     5521 ctgcgcgggg ccgcggtatt tcgctacttc agtccggcgg cgatcgtggg aatcaaagtg
     5581 ggagtcggcg ttttgctggt gacctcggcg cttgaaggag cgctcgggtt atcgccgatg
     5641 acgacgaaat ttttctatga aaagttttac attgcgatcg gttcgtggca ggacctcgtt
     5701 aatccctatg cggcaatcat tagcgccatc accatcggct caggcttgct gttgaagaca
     5761 agttggccgc gcagctacat catcggcgcg gtagtaatcg gcagcctggt cggtggagca
     5821 ctgtacggct ggctcggccc ggtgcagtca cagttggaac tgcttggcca cgtcaccttg
     5881 cacgccctgc cactcaatat gccgcgactg gggccgcagc attggctgtt cgtcgagcaa
     5941 gttgtgcccc aggcagcggc catcgccgtg ctgggccttt cgcaatccct ggtcatcgct
     6001 cgcgatctga aagcgaccgt cgcccacggt ctaaatctgc acaaggaagt tttcgcacaa
     6061 ggtattgcca acgtattaag tcccttcttt tccacttttg caggctcggg cagtttcaac
     6121 cgcaccagtg ttgctcacga gatgggagcg cgtacgcccc tcgccggtat catcgcagcc
     6181 ttcgcagtag tgctcatcgc atggacaatc ggaccgcttt taacgtggct gcccatgccg
     6241 gtcatcgccg gcgtcatggc gctcgtcgga atcggcatgg tccagggcaa ccagatcgcg
     6301 ccattgcgca accgcatcga cggcacggta ttcctgctaa ccctgctggt ggttgttttc
     6361 gtgggcctgg agtacgggat tctggtcgcg gtgcttgctt cagtgggctt tttcgtcgcc
     6421 agtgcctcac gcgtgaagtt atcgatttcg cacgatgctg gtaaacagca tatcgccgta
     6481 acaggcaatt tgttttacgc gtccctcgac agtctttcgg attgcctgcg cgccgatcca
     6541 accgcataca ccgtcctgga tctgagtcga gtgccctact gcgatgcctc cgctacggaa
     6601 atgattgaga ccatccagcg cgacaggctg cgacacggcg ggcaactgct ggtcacggca
     6661 ggcgcatagc gctggtttcc gccagacgat tgatcagcgt cactccagct tgaagacgat
     6721 ttcctgatcg gcctcgtaac tcgtgccgtc gcgtttgaag atgtattgaa ccaccgcctc
     6781 catgactgcg gcgtcgaata cgcccggtgg atccgcagcc agaatcctgg cagcgctcac
     6841 actgccgttc acttcaaccg tcagccgcac acgcaccctg ccttcaatat gcttggcttc
     6901 cgcgtctggc ggatacttcg gttttatttt tttaaccggc ttaacgtcac ggaacacggt
     6961 cggcagcggc tgcgcctctg gctttggttc aggtaccggt tcgggtggct tttcttcttc
     7021 ctttggcttc ggctcaggtt tcggcttggg ctcgaccttg ggcaacacga ttttcgactg
     7081 cacctcattc ggcagcggct tcggaacgac gacgggcgtt tgcttgatct tgcgctcggg
     7141 ttttggtttc tgcttcttcg gcggctcggg tcgcggctct tcgggcgccg gttcttccag
     7201 gcgtaccgac atcac
//
LOCUS       DATMZQ010000332         3630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2209069, whole genome shotgun sequence.
ACCESSION   DATMZQ010000332 DATMZQ010000000
VERSION     DATMZQ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209069"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VN496_10550"
     CDS             complement(<1..812)
                     /locus_tag="VN496_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17436.1"
                     /translation="MSIADSASANGLLSTPLVLPAPLAGALAALVLVIVLVLVRRSEG
                     AIVSRLLILVSVIAVCILVTTAILDRLEINDRTAEQRALLQRDAQLSMSAITSGSLAC
                     LDGVAGEQVENACEQAVFADAQSTAKAVAYISARLSLLADVAVTARGDRDFLAAFATV
                     RRAIELDRYGIAAHVLSVRDACTAERCTALAMFEDTGALKANLKVRAFDTYVTRYASA
                     WNKREPLADKEPQTSSVTPAPVANAAEPSVAHPLDPKYDFPSSASIPAVSIM"
     gene            complement(822..1784)
                     /locus_tag="VN496_10555"
     CDS             complement(822..1784)
                     /locus_tag="VN496_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXF17437.1"
                     /translation="MRAFLRLAAVVFAILQVWPTAAQNYPSRPVKFIVPFGAGGPADV
                     FARVLAQHLSEETKQSFIVEDRPGAGSIIGTDAVAKSAPDGYTLLVMSNTHTTNESLL
                     PNKPFQLMRDFVPVATINYSDLLMVVHPSVPAKTVKEFIELAKKDPGKLNYASSGPGT
                     PYHMAGELFKAMSQTDIVHVPHKASGDARNSVVSGTVQMMFDAVTTMTELAKAGQVRA
                     LGTTGLTRSQLTPDLPTIAETVPGYEATIWLGVMAPKGTPKEIINFLNTNINKVINRP
                     DVKEAWLKQGAIPLIKTPDEFEAFLVKDIEKWANVVKVSGAKIQ"
     gene            complement(1836..2171)
                     /locus_tag="VN496_10560"
     CDS             complement(1836..2171)
                     /locus_tag="VN496_10560"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXF17438.1"
                     /translation="MPIYITQGRYTREAVRGMIVKPEDRADAVARILSKIGGRLLGYY
                     LTFGEYDFLTIAEAPNDVQMAAVLLAAGSGGGVTGLKTTVALTSVEAKGAFAAASDLA
                     PGFKSAGGT"
     gene            complement(2265..2696)
                     /locus_tag="VN496_10565"
     CDS             complement(2265..2696)
                     /locus_tag="VN496_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17439.1"
                     /translation="MSKHSAKTAGRAAKTTSRTEKKSAKSVRVTAKKTSARLPVGKAT
                     RPARTATPAIASAKPTKMIRTSSPPVQPTAMHELPLRLHKYQVGETVYYTSPTFGRAA
                     ATGSYIVVKLLPSEGDDYQYRIKSSGEAFERVARESQLDRV"
     gene            2920..3126
                     /locus_tag="VN496_10570"
     CDS             2920..3126
                     /locus_tag="VN496_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17440.1"
                     /translation="MRVLIGIILGVIITVGGAYLYDSRHTVTSSDSATSAQRPMVNWD
                     VVSNKWDHLTARARAEWSRLAAKT"
     gene            complement(3144..3380)
                     /locus_tag="VN496_10575"
     CDS             complement(3144..3380)
                     /locus_tag="VN496_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17441.1"
                     /translation="MHYRVYSGPKGSPDLSPLEKSQLLFKEFTVLDDALSWARHLEGT
                     GRIPLLIEGDDGTRMDRRAIINALRVGIREQLGD"
BASE COUNT          740 a         1079 c         1040 g          771 t
ORIGIN      
        1 ctcatgatgc tgaccgccgg aatcgaggca gacgaaggga aatcgtattt cggatctagt
       61 ggatgggcca ctgaaggctc ggcggcgtta gcgaccggcg ccggcgtcac tgaggacgtc
      121 tgcggctctt tatcggctaa aggctcccgt ttgttccaag cggatgcata ccgggtgaca
      181 taggtgtcga aggcgcgaac cttcagattt gccttgagcg ctccggtatc ctcaaacatg
      241 gccaaggctg tacagcgctc tgcggtgcat gcgtcgcgca cagagaggac atgagcggcg
      301 atcccatagc gatcgagttc aatggcacgg cgcacagtgg caaacgcggc caggaaatca
      361 cgatcgccgc gtgcggtcac agcgacgtcg gcaagcaacg acagccgcgc gctgatgtag
      421 gctactgcct tggccgtgct ctgggcgtca gcaaacaccg cttgttcgca ggcgttctca
      481 acctgttcac cggcgacacc atcaaggcag gcgagcgaac cagaggttat agcgctcatg
      541 ctgagctgcg catcgcgttg caggagcgct cgctgctcgg ctgtccggtc gtttatctcc
      601 agtcggtcga ggatggcggt tgtcaccaaa atgcagaccg cgataactga gaccagaatc
      661 agcaggcgcg aaacgatggc gccttccgag cggcgcacca acaccagcac gatcaccagc
      721 accaatgccg ccaacgcacc ggccaaggga gccggcagca ccagcggcgt tgaaagcagc
      781 ccgttcgcag acgcgctatc ggcgatcgac atgggttcca gctattgaat cttcgctccc
      841 gagactttta ccacattcgc ccacttttcg atgtccttta cgaggaacgc ttcaaattca
      901 tcgggcgtct tgatgagcgg tatcgctccc tgcttgagcc acgcttcctt cacatcgggc
      961 cggttaatga ccttgttgat attggtgttg aggaaattga tgatctcttt gggcgtgcct
     1021 ttcggcgcca ttacaccaag ccagatcgtc gcttcatagc cgggtaccgt ttcggcaatc
     1081 gtcggaaggt ccggggttaa ctgcgagcgt gtaagacccg tcgttccgag tgcacgcacc
     1141 tgcccggcct tggcaagttc cgtcatggtc gtcacggcat cgaacatcat ctgcacagtc
     1201 ccggaaacca cgctgttgcg cgcatcgccg gaagccttgt ggggaacgtg cacgatgtcg
     1261 gtttggctca tcgctttgaa gagctcacct gccatgtgat agggcgtgcc tggaccggaa
     1321 gaggcgtaat tgagcttgcc cggatccttc ttcgccagtt ctatgaattc cttcacggtt
     1381 tttgccggaa cggatggatg aaccaccata agcaggtccg agtagttgat tgttgctacg
     1441 ggaacaaagt cacgcatcaa ctggaacggc ttgttgggaa gcaaagactc attggtcgtg
     1501 tgggtgtttg acatcaccag aagcgtgtac ccgtcaggcg cagactttgc gaccgcatcg
     1561 gtgcctataa tcgaacctgc accgggccga tcctcgacaa tgaatgactg cttggtttcc
     1621 tccgaaaggt gctgagcaag cacgcgagcg aatacgtcgg ccggcccacc cgctccaaag
     1681 ggaacgatga atttaacggg tcggctcgga tagttctgtg ccgcagtcgg ccagacctgc
     1741 aagatcgcaa acaccactgc agccagacgc aaaaaggcgc gcataaatcc tcccctcaaa
     1801 agtttattcg ttgactacgt cgtagttttt tctcgtcatg tcccgccggc ggatttaaac
     1861 cctggcgcaa gatcgctcgc cgcggcaaat gctcctttgg cctcgaccga cgtcagcgca
     1921 acagtcgttt tcaagcccgt cacacccccg ccacttcctg cggccaatag cacggcggcc
     1981 atctgaacgt cgttgggagc ttcggcaatg gtcagaaaat cgtattcgcc aaatgtgagg
     2041 tagtaaccca atagccgccc accgattttc gacaagatcc gtgcaacggc atcggctcgg
     2101 tcctccggtt ttacgatcat cccccttacg gcttcgcggg tgtaacgccc ctgcgtgatg
     2161 taaatcggca tgcccggcct ccttcgcgca gcaaactgca tcgttccggt catcatatgg
     2221 aacggccacg gcaaaatagc gacaggaggt cccgcagcag atggtcaaac gcgatcgagc
     2281 tggctctctc tggccacgcg ttcgaacgcc tcgccggaac tcttgatccg gtactgatag
     2341 tcgtcaccct cggacggcag gagcttgacc acgatatagc ttccggtggc cgcagcgcgc
     2401 ccgaacgtcg gcgacgtgta gtagacagtc tcgccaacct ggtacttatg caaacgcagc
     2461 ggcagctcgt gcatagctgt tggctgcacc ggcgggctag atgtgcggat catttttgtg
     2521 ggttttgccg aggcaatagc cggcgtggcc gtacgtgctg gccgcgtggc cttgccgacc
     2581 ggcagacgag ccgatgtctt ttttgccgtc acccgaacgc tcttcgcgct ctttttttcg
     2641 gtccggctcg tcgttttggc cgcccgcccg gcggttttgg ctgaatgctt gctcataatt
     2701 tcaatatagc agctaatttt gtttgcgggc aaagtttttt ggcgtagaag cttaatgttc
     2761 tcaatgagtt ataacacaac atgggtcggt acaaggttaa catcgcgtgc cccaaaatgc
     2821 acctgcttgg ccgtcacaat tgcctgcgga cccctggcaa cggagaggga acccaccgtc
     2881 gccagcggcg tttgaggtcg ggaaccagga gttttcatca tgcgtgtcct tatcggcatc
     2941 atccttggcg tcataatcac ggttggcggc gcttaccttt atgactcacg ccatacggtg
     3001 acgagttccg acagcgcgac cagcgcgcaa cgtccgatgg tgaattggga tgttgtcagc
     3061 aacaaatggg accacctcac cgcgcgggcg cgtgccgagt ggagccggct cgccgccaag
     3121 acctgaacga ttgcccgggc tgactagtca cccagttgct cacggatccc gacccgcaac
     3181 gcattgatga tggcgcgccg gtccatacgc gtgccgtcgt cgccctcgat caatagcggg
     3241 atgcggcccg ttccttccag atggcgcgcc cacgatagag cgtcatctag aaccgtgaac
     3301 tccttgaaca acaattgcga tttctcaagg ggcgacaaat cgggcgaacc cttggggcca
     3361 ctgtagacac ggtagtgcat cgcgaacctc ttggctttgc cacccacccc attgttccgg
     3421 actaacgcct gacaggcatg tggggttcat catcccatat tgcatgggtc gccgtgctat
     3481 atgtcccccc gtcaaggggg gttgcccatg cgagcggcgt actatgagcg taacggcact
     3541 gcgcgcgagg tcttacaggt cggcgagatc gaaacgcctc acgcgggacc cggcgaagtc
     3601 cgcgtcaagc ttgccgcctc cggcgtgaac
//
LOCUS       DATMZQ010000333         3416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3037186, whole genome shotgun sequence.
ACCESSION   DATMZQ010000333 DATMZQ010000000
VERSION     DATMZQ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3037186"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="dapB"
                     /locus_tag="VN496_10580"
     CDS             <1..798
                     /gene="dapB"
                     /locus_tag="VN496_10580"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXF17442.1"
                     /translation="QQIAVAGSSGRMGRALIEAIAQSADFRLKAALELAGSDCLGRDA
                     GELVGKPCGIPVTADLGKVLPGCDVLVDFTRPEGTLHHVELCRRLGIRMVIGTTGFQS
                     AQKAVVVDAAKTIPIVMAPNMSIGVNLTFKLAELAAKVLNEDYDVEIIEAHHRLKIDA
                     PSGTALRLGEIIAAALGRDLAKDAVFGREGVTGERKQSTIGFATVRGGDIVGDHTVLF
                     AGSGERVEITHKAGSRATFAHGALRAARYLAGKTSGLYDMQDVLGLK"
     gene            931..2076
                     /gene="carA"
                     /locus_tag="VN496_10585"
     CDS             931..2076
                     /gene="carA"
                     /locus_tag="VN496_10585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXF17443.1"
                     /translation="MPALDCQPAILVLADGTVFRGVSTGAQAHTSGEVVFNTAMTGYQ
                     EILTDPSYCRQIVTLTYPHIGNVGVNPEDTESGKVFAAGLVVRDVPIRASNFRQSQHL
                     SAYLMDHGIPAISGIDTRKLTRILRDKGAQSGCLMSGKVDEAFALEQARAFPGLAGMD
                     LAKVVSCKKPYQWSESEWALGKGYRTEQNSRYHVVAYDYGVKRNILRMLSGRGCKLTV
                     LPAQSSAEDALALKPDGVFLSNGPGDPQPCEYAIRAIGKLVNAGLPTFGICLGHQLLG
                     LASGARTVKMKFGHHGANHPVQDVESGRVMITSQNHGFAVDAATLPHNVKVTHVSLFD
                     GSLQGFSRTDRPAFCFQGHPEASPGPHDIGYLFDRFIGLMEQHKAHR"
     gene            2090..>3416
                     /gene="carB"
                     /locus_tag="VN496_10590"
     CDS             2090..>3416
                     /gene="carB"
                     /locus_tag="VN496_10590"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXF17444.1"
                     /translation="MPKRTDIKGVLIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPDMADVTYIEPINWQTIEKIIAVERPQAILPTMGGQTALNCALDL
                     AKHGVLEAYGVELIGASKDAIDKAEDREKFKQAMTRIGLASPRSSIAHSLEEALQVQS
                     MVGFPTIIRPSFTLGGTGGGIAYNKEEFLAICERGLDASPTHELLIEESVIGWKEFEM
                     EVVRDRKDNCIIICSIENLDPMGIHTGDSITVAPAQTLTDKEYQIMRDASIACLREIG
                     VDTGGSNVQFGIHPKDGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNDITGGTTPASFEPTIDYVVTKIPRFAFEKFPQADSRLTTQMKSVGEVMAIGRTFQ
                     ESMQKALRGLETGIDGFDEKTNDRELIEAELADAGPDRMLFVADAFRNGM"
BASE COUNT          752 a          945 c         1029 g          690 t
ORIGIN      
        1 cagcagattg cagttgccgg tagttccggg cgcatggggc gggcgttgat cgaggcgatc
       61 gcgcagtcgg cggattttcg tctgaaggcg gcactcgaat tggccggtag tgattgtctc
      121 ggcagagatg ctggtgaatt ggtgggaaag ccgtgcggaa tccccgtcac tgcagatctc
      181 ggcaaagtgc tgcctggctg tgatgtgctg gtcgatttca ctcggcccga aggtacgttg
      241 catcacgttg aactgtgccg caggctaggc atcaggatgg tcatcggcac aaccggtttc
      301 cagtctgcgc aaaaggccgt ggtggttgat gccgcgaaaa ccattccgat cgtaatggca
      361 ccgaacatga gcatcggcgt caacctgacc ttcaaactgg ccgaactggc ggcaaaggtg
      421 ctgaacgaag actacgacgt cgagatcatc gaagcgcatc atcgactcaa aatcgatgcg
      481 ccgtccggta ccgcgctgcg cctgggcgag atcatcgctg ccgcgcttgg gcgcgatctc
      541 gcgaaagacg ctgttttcgg ccgcgagggt gtcaccggcg agcgtaagca gtccaccatc
      601 ggattcgcaa cggtgcgggg cggagacatt gtaggcgatc acaccgtact gtttgccgga
      661 agcggcgagc gagtggaaat tacacacaag gctggaagcc gtgcgacatt cgcacacggg
      721 gccctgcgcg ccgcgcgtta cctcgccggc aaaacctctg gtctttacga catgcaggat
      781 gtactgggcc tgaaatgatc cgctgagccg gaccggccgc attgaatgat cgtggtcgat
      841 ccactataat cttgggccaa gaaagcggga ggggcgtgcg cctctcccgc tttccatgtg
      901 tagtttcgaa acgcactccc aggagccctc ttgcctgccc tcgattgtca gcccgccata
      961 ctcgtgctcg cagatggcac ggtatttcgt ggcgtgtcaa ccggtgcaca ggcgcatacg
     1021 tccggtgagg tggtgttcaa caccgcaatg accggatatc aggaaatcct gactgatcca
     1081 tcctattgtc ggcaaatcgt tacgctgacg tacccccaca tcggcaatgt cggcgtgaat
     1141 ccagaagaca ccgagtccgg gaaagttttt gcggccggac tggttgtgcg cgacgtcccg
     1201 atccgcgcca gcaattttcg ccaaagccag cacctatccg cctatttgat ggatcacggc
     1261 atcccggcaa tcagcggcat cgatacgcga aaacttacgc gaatattgcg ggacaaaggc
     1321 gcgcagagcg gttgtctgat gagcggaaag gtggacgaag cgttcgcgct ggagcaggcg
     1381 cgcgcgtttc ccggccttgc cggcatggac ctcgcaaaag tcgtcagttg caaaaagccc
     1441 taccaatgga gcgaatcgga atgggcgctg ggcaagggct atcgcacgga gcagaactcg
     1501 cgatatcacg tcgtggctta cgactacggc gtgaagcgca acatcctgcg tatgctttca
     1561 ggccggggct gcaagctgac cgtgctgccg gcgcaaagct ccgctgaaga tgcgctggcg
     1621 ctgaaaccgg acggtgtttt tctttccaac ggtccgggcg atccgcaacc gtgcgagtac
     1681 gcgattcgtg ccattggcaa actcgtgaat gcgggcttgc cgacatttgg tatctgttta
     1741 gggcaccagc tcctgggcct cgccagcggc gcacgcacgg tgaagatgaa gttcggccat
     1801 catggcgcta atcatccggt gcaggatgtt gaaagcggcc gcgtgatgat taccagccag
     1861 aatcacggat tcgccgtcga tgcggcaacc ttgccgcaca atgtgaaagt tacccatgtc
     1921 tcgctgttcg acggcagcct gcagggtttc agccgcactg acagaccggc gttctgtttt
     1981 caggggcatc cagaagccag ccccggcccc cacgacattg gttacctgtt cgatcgtttt
     2041 attggcttga tggaacagca caaggcgcat cgctgagcca tacagacaca tgccaaagcg
     2101 aaccgacata aagggcgtgc tcatcatcgg cgcgggtccg atcgtcatcg gccaagcttg
     2161 cgaattcgat tattccggcg cgcaggcctg caaggcatta cgtgaagagg ggtacagggt
     2221 gatcctggtg aattccaatc cagcgaccat catgactgat ccggatatgg cggatgtcac
     2281 ctacatcgag ccgatcaact ggcaaacgat tgaaaaaatc atcgcggtgg agcggcccca
     2341 ggccatattg ccgaccatgg gcggccagac cgcgctgaat tgcgcgctcg atctggcgaa
     2401 acatggcgtt ctggaagctt acggcgtgga attaatcgga gcttccaaag acgccattga
     2461 caaggcagag gatcgtgaga agttcaaaca ggcaatgacc cgaattggcc tggctagtcc
     2521 gcgttcgtcc atcgcgcaca gtctcgaaga ggcgttgcag gtgcaatcca tggtcggttt
     2581 cccgaccatt atccggcctt catttacgct aggcggaacc ggtggcggga tcgcttacaa
     2641 caaggaagaa ttcctggcta tctgcgaacg cgggctggat gcgtcgccta cgcacgagct
     2701 tcttatagag gaatctgtca tcggatggaa agaattcgag atggaggtgg tgcgcgaccg
     2761 caaggacaac tgcatcatca tctgctccat cgaaaacctc gatccgatgg gtatccacac
     2821 cggtgactcg atcactgttg cgccagcgca gaccctgacg gataaggaat atcagatcat
     2881 gcgcgatgca tcgattgcat gcttgcgcga aatcggcgtg gacactggcg ggtcgaatgt
     2941 gcagtttgga atccatccga aggatgggcg catggtagtg atcgagatga atcctcgtgt
     3001 ctcccgttcc tcagcgcttg cttcgaaggc aaccgggttc cccatcgcca aggtggcggc
     3061 caagctggca gtcgggtaca ccttggatga actcaggaac gacatcacgg gcggcactac
     3121 gcctgcctca ttcgagccga ccattgacta tgtcgttacc aagattccgc gttttgcatt
     3181 cgagaaattt ccgcaggccg attcacgtct gaccacgcag atgaaatccg taggcgaagt
     3241 catggccatt ggccgaactt tccaggagtc gatgcaaaaa gcactgcgtg gactcgaaac
     3301 gggcattgat ggattcgacg aaaagacaaa cgaccgagag ttgatcgaag ccgagttggc
     3361 cgatgccggg ccggatcgca tgctgttcgt tgcagatgca ttccgcaacg gcatgg
//
LOCUS       DATMZQ010000334        12142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_275881, whole genome shotgun sequence.
ACCESSION   DATMZQ010000334 DATMZQ010000000
VERSION     DATMZQ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275881"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VN496_10595"
     CDS             <1..862
                     /locus_tag="VN496_10595"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HXF17445.1"
                     /translation="HPRACMADYWTTDWLNRSDIFAPLRAVGAGLPKIGWPNPELLNA
                     LADNAGRVVNAQGLRVRFVTQQNKSNDFREGFEQRAFLKGEVQHRPLDWHDLFNALVW
                     MTFPTSKAVINARHYELLAGESVAGESAEPLDPLQVGRRPPERDALTLFDEDGIVVAS
                     SDAGLLELLRAFRWKELFWNRRELVQAHMRFFVFGHALYQKALAPFIGMTGKAVLLHV
                     PPDFAQYSLQCQTTSADRLLAAHIWDRTHMRQGRELAPLPVLGVPGWWPDNERESFYD
                     NARYFRSGRR"
     gene            complement(879..1943)
                     /locus_tag="VN496_10600"
     CDS             complement(879..1943)
                     /locus_tag="VN496_10600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXF17446.1"
                     /translation="MLTKTNIGLTIEHDELKGFSRTIAEIEWLLLCLVLVYLLAVGPP
                     YEGATAINTALFFFAAFILGLHYVHFYKLENRSKLMFETWVMIVFITWVVWYTGQINS
                     PLLSLYLLPIIASALIFGKVPTTVQAVAIIGCYMFFSYNAKTKTLLTMPFWGELLAIS
                     APMILVAYITTMLSADIRFAVDKIKQVSDTDELTGTYNMRAFSSVLQRTFRQSVRYGH
                     PFSVVMIDSDNLKQVNDKHGHESGNRLLQHLVRSIREQLRGSDVMARFGGDEFILLLP
                     ETANKGALEMAERIRKAVENSRFDVRSDDSHVTVSIGVASYPDDGPNLEVIVEKADKA
                     MYRAKQNGRNRVSAYEKDSV"
     gene            2385..2807
                     /locus_tag="VN496_10605"
     CDS             2385..2807
                     /locus_tag="VN496_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HXF17447.1"
                     /translation="MKALKGSKTEKSLKDAFAGESQANRRYLYFAAKADVEGQNDVAS
                     IFRSTAEGETGHAHGHLEYLESVGDPATGLPIGRSRDNLKAAIAGETHEYTDMYPGMA
                     KNARDEGFEEIADWFETLAKAERSHANRFQKALDALTD"
     gene            2892..4235
                     /locus_tag="VN496_10610"
     CDS             2892..4235
                     /locus_tag="VN496_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HXF17448.1"
                     /translation="MTVREGSLEAPTRHPIDWQSAEFYDEEKAHAEMHRIFDICHGCR
                     RCVNLCTAFPTLFDLIDEGKTGELDGVDKGDYGKVVDKCYLCDMCYMSKCPYVPPHPW
                     NVDFPHVMLRAKAVKFKKGETVLRDRLLTSTDAVGKLASIPVVVQMVNAANKARSART
                     LMQSALGVHKDRVLPEYTSAHFRKTARPQSSFAVKDGKNAPGKAAIFSTCYVNYNEPG
                     LGHDLLKILEHNEVPAIVVQKEACCGMPKLELGDLATVAKLKQANIPVLANLAREGYA
                     ILTAVPSCTLMFKQELPLMFPHDEDVKAVQEAMFDPFEYLVMRNRDGLLKTDFTTPLG
                     KISYHIPCHLRVQNMGQKTRELLELVPRTEINTVERCAGHDGTWGVKTEFFADSMKIG
                     RPVFRQMASTEPDYVSSDCAIAGRHIAQGIADGGLAQRPTEKKHPLTLLRMAYGI"
     gene            4258..4845
                     /locus_tag="VN496_10615"
     CDS             4258..4845
                     /locus_tag="VN496_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HXF17449.1"
                     /translation="MGQITAESLMSLEQYAKNRKEFRAKVLAHKKDRTVRLGEHVTLV
                     FEDELTMRYQIQEMLRVERIFEQDGIRDELDSYNPLVPDGSNWKATMLIEYPDSAERR
                     IALSKLIGIEDRVWVEVEGQARIFAIADEDIDRENNDKTSAVHFLRFELPAVVKQALR
                     AGAGLSVGVDHPNYSALIGKAGPATVRCLLKDLVF"
     gene            4938..6143
                     /locus_tag="VN496_10620"
     CDS             4938..6143
                     /locus_tag="VN496_10620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXF17450.1"
                     /translation="MLESRMIGMLENRLKRAGLPISMTLWNGDTIESGPGANLSLTVK
                     SPQALMSLANPSLGGIARAYVEGQLDLDGDIRQTIRAGEGLVSGQSTTYQNRPKTWKW
                     WRHTRPADRKNVQHHYDVGNDFYGLWLDRNRVYSCAYYKTPEDTLDQAQEQKLEHICL
                     KLDLKPGERFLDIGCGWGGLILWATKNHKVSALGITLSEEQHAYATQKIREFGLESRC
                     EVRLMDYRDVEEGSPFDKIASVGMFEHVGKRNLNAYFAKISRLLKPGGMVMNHGITTN
                     SLEDAELGSGIGEFVDEYVFPGGELVHVSRVLSEMSQQGLEPWDAECLRPHYARTLWE
                     WVRRLESRQDEARRIVGEKLYRIWRIYMAGSAHAFDRGWISIFQILGTKSLSDGSVSY
                     PLTRNHVYR"
     gene            6262..6729
                     /locus_tag="VN496_10625"
     CDS             6262..6729
                     /locus_tag="VN496_10625"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HXF17451.1"
                     /translation="MTKDIGGVPEEKEFKEVDSEFPAYPPDSGLLEFYPRQKSQNRFY
                     VDPNTLSIGPDRVIRYSLVVKSPSGALTVSYEGLRCKTSEYKLYGFGVTAGEWTRSRN
                     SEWHPVPKTAGDFRFALYKDYFCDLEAIAGRNERDLIANLAGNPLNNVTDKNR"
     gene            complement(6790..7908)
                     /gene="proB"
                     /locus_tag="VN496_10630"
     CDS             complement(6790..7908)
                     /gene="proB"
                     /locus_tag="VN496_10630"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXF17452.1"
                     /translation="MKSVAANAVRLVVKVGSSLVTNEGQGLDHAALARWAEQIAELTR
                     RKKEVVLVSSGAVAAGMQRLGWKRRPNALYELQAAAAVGQMGLVEAYESCFRKHGLHT
                     AQVLLTHEDLADRKRYLNARSTLRTLLDLGVIPVINENDTVATDEIRFGDNDTLAALV
                     TNLIEADALLILTDQPGLYTKDPRRNNDATLVTDARAGDAQLEKMAGGIGSHLGSGGM
                     LTKVLAAKRAARSGASTVIASGREPDVLIRLASGERIGTQLVAETMTLAARKQWMADH
                     LQVRGKLKLDDGAVRALRSEGKSLLPIGVVEAAGDFERGEMVSCLDSRDCEIARGLVN
                     YSSVETRKILKRPSHEIEARLGYIDEPELIHRDNLVLL"
     gene            complement(7905..8987)
                     /gene="obgE"
                     /locus_tag="VN496_10635"
     CDS             complement(7905..8987)
                     /gene="obgE"
                     /locus_tag="VN496_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXF17453.1"
                     /translation="MKFIDEAIIEVHAGKGGDGSASFRREKFIPRGGPDGGDGGRGGS
                     ILAVADRNINTLVEFRYARIHRARNGEKGRGSDCYGKAAEDVILRFPVGTVVTDLESG
                     EVLADLSVHGQTAVLAKGGQGGLGNIHFKSSTNRSPRQFTPGTLGENRRLKLELKVLA
                     DVGLLGMPNAGKSTYIRSVSAARPKVADYPFTTLHPNLGVVRVDENRSFVIADIPGLI
                     EGASEGAGLGHQFLRHLQRTRLLLHLVDLAPFDVDADPAKDARAIVEELRKYDEALFQ
                     KPRWLVLNKVDLIETQDRDSRIKAFLSAYARLVSEPEKTFIISALTGEGGRELTFAIM
                     EHLERYRRPDAETEIADGRTEDRPAP"
     gene            complement(9140..9397)
                     /gene="rpmA"
                     /locus_tag="VN496_10640"
     CDS             complement(9140..9397)
                     /gene="rpmA"
                     /locus_tag="VN496_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXF17454.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKRYGGEVIPAGSIIVRQRGTQ
                     VHPGENVGIGKDHTLFAKITGTVTFVTKGPFKKKTVSVLPA"
     gene            complement(9400..9717)
                     /gene="rplU"
                     /locus_tag="VN496_10645"
     CDS             complement(9400..9717)
                     /gene="rplU"
                     /locus_tag="VN496_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXF17455.1"
                     /translation="MYAVIKTGGKQYRVSPGEKIKVEQLPADIGSQVALDVLMIADGE
                     KVSIGTPVLAGASVQATVVSHGRHDKVHIFKMRRRKHYQRHGGHRQNYTEIEIGAMSA
                     QGN"
     gene            9919..10887
                     /gene="ispB"
                     /locus_tag="VN496_10650"
     CDS             9919..10887
                     /gene="ispB"
                     /locus_tag="VN496_10650"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HXF17456.1"
                     /translation="MGPEIINSLIAEDLKQVDAVISHRLSSDVVLIRQVADYIIASGG
                     KRLRPALLILSARACGYQGTHHHELAAVVEFIHTATLLHDDVVDDSHMRRGRATANSL
                     FGNAAAVLVGDFLYSRAFQMMVGVRSMRIMEVLADATNNIAEGEVMQLLHVRNANVNE
                     QDYLRVIRAKTAKLFEAAGRLGAILAAVPPAQENALASYGMHLGTAFQLVDDILDYSG
                     DLMQTGKNLGDDLAEGKTTLPIIHAMRHGSSEQETLIRNALVSGGLSEFEPVMEAMRE
                     TGALDYTRMQAEASARHARDSIAVLTDSKYKDSLLELATFAVTRKY"
     gene            10898..10974
                     /locus_tag="VN496_10655"
     tRNA            10898..10974
                     /locus_tag="VN496_10655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10932..10934,aa:Pro,seq:cgg)
     gene            11001..11273
                     /locus_tag="VN496_10660"
     CDS             11001..11273
                     /locus_tag="VN496_10660"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HXF17457.1"
                     /translation="MAKFLLVIAVFIVVYLLLRNVANKPGAASARDTASNGSAAKKGA
                     SKLAEDMVRCGICGVHLPTSEAITSRGDFFCSKEHLQLAVRQRDSH"
     gene            11326..>12142
                     /locus_tag="VN496_10665"
     CDS             11326..>12142
                     /locus_tag="VN496_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXF17458.1"
                     /translation="MDRRQYGDAFWRSLFYFNVYRLAAALVLLAAVLALGGEVPFGSN
                     SRHAYLYSNVCYIVFSVMAFGAVLSRKPYFTGQLAVQICADILFLTTMMHFSGGISSG
                     LGLLLLVSLAAVGLISRGRLTLFFAALATIAILGEHTYEVVFLNGSISQYAQAGLLSI
                     GFFATGWLAHSLAKRAVAIEAIAAQREIDLANLAQVNQLVIQDMQDGVLVVDERGVIR
                     QINSRAEAILGPLRRSNREVLLQDYAPTVAARLEQWRRDPGAGFDPLRTVQTNK"
BASE COUNT         2622 a         3345 c         3452 g         2723 t
ORIGIN      
        1 gcatccgcgc gcttgcatgg ccgactactg gaccaccgac tggttgaatc gctcggacat
       61 ctttgcgcca ctacgcgcgg ttggtgccgg ccttcccaaa atcggctggc ccaatcccga
      121 gcttctgaat gcactggcgg ataatgccgg aagagtggtg aatgcacagg gtctgcgcgt
      181 ccggtttgtc acgcagcaaa acaaatcgaa cgacttccgg gaaggttttg agcagcgtgc
      241 gttccttaaa ggtgaagtgc agcaccgccc gctggactgg cacgacctgt tcaacgcttt
      301 ggtgtggatg acattcccga ccagcaaagc ggtgattaat gcacggcact atgaattgtt
      361 ggctggcgaa tcggttgcgg gggaatcagc ggaaccgttg gaccctttac aagttggccg
      421 tcggccgccc gagcgtgacg ccttgaccct gtttgacgaa gacggcattg tcgttgcatc
      481 aagtgacgca gggttgcttg aactgctgcg cgcatttcgc tggaaagaat tattctggaa
      541 tcggcgcgag cttgtccaag cgcacatgcg ctttttcgtg tttggccatg cgctttacca
      601 aaaagccctg gcaccgttca tcggcatgac cggcaaggcg gtgttgttgc acgtcccgcc
      661 ggattttgcg cagtactcat tgcagtgcca gaccaccagc gcagaccgcc tgcttgcggc
      721 ccatatatgg gaccgcacgc atatgcgcca aggacgtgag ttggcgccgc tgccggtgct
      781 cggtgtgccg ggatggtggc cggacaatga gcgggaaagc ttttacgaca acgctcgcta
      841 tttccgttcg ggtcgccgct agctcgtggt tggccgcatc agactgaatc tttttcgtag
      901 gcactgacgc gattgcggcc gttctgcttc gcccgataca tcgctttgtc cgccttttcg
      961 acgatgactt ccaggttcgg tccatcatcg gggtagcttg caaccccaat gcttacggtg
     1021 acatggctgt catcgctgcg cacgtcgaat cgcgaatttt cgacggcctt gcggatacgt
     1081 tccgccattt ccagcgcgcc tttattggca gtttcaggca gcaacaagat gaactcgtcg
     1141 cccccaaacc gcgccatgac gtcggatcct ctcaactgct cgcgtatgct gcgcaccagg
     1201 tgctgcagca atctgtttcc tgattcatgt ccgtgcttgt cgttcacctg cttcagattg
     1261 tcggaatcaa tcatcactac ggaaaaggga tgaccatagc gaaccgactg acgaaatgtc
     1321 ctttgcagca cggaagaaaa tgcgcgcatg ttataagtgc cggtcagttc gtcggtgtcc
     1381 gacacctgct ttatcttgtc gacggcgaaa cggatgtcgg cagacagcat cgtggtgata
     1441 taggcaacca ggatcatggg cgcggaaatg gccagcagct cgccccagaa cggcatggtc
     1501 agcagtgttt tggtcttcgc gttgtaggaa aaaaacatgt aacagccgat gatcgccact
     1561 gcctgtaccg tagtcggcac tttgccgaaa atcagtgcgc tggcgatgat gggcaacagg
     1621 tacaagctga gcagcgggct gttgatctgc ccggtatacc agaccaccca ggtgatgaat
     1681 acgatcatca cccaggtctc aaacatcagt ttcgaacggt tttcgagctt gtagaaatgc
     1741 acatagtgca agcccagaat aaaagcggcg aagaagaata gcgcggtatt aattgcagtg
     1801 gcgccctcgt aaggcggccc aacggccagc aggtaaacca gcacgagaca gagcagcaac
     1861 cattcgattt ccgcaatggt cctcgaaaag cccttgagtt catcatgttc tatggtcaag
     1921 ccgatgtttg ttttggtaag catcgggccg ccggtgtgcg gagcttctcg ttcgccgttc
     1981 cacatggttg tttactttcg cgcaagtggg gtaagactaa acggtttcac tggccagtac
     2041 cagaaggttg acctatgcgt tcggtctaaa taaaacgcag tcttgataac tatgcttaca
     2101 ccagtaaagg cgcgtagtcc cagggcagtt gactgctgaa tctagccagc gatgcattgc
     2161 gcagcgctcc tggaatgcca atcgattgcg cccgcctagc cttcatcggg ttggaaaatt
     2221 caaatgcttg agcaaaccgc tcgcttgcgg tacgccggtg cgcaagttga cttggtcagc
     2281 ggcaatccat acacttaatt tacagattag attaagtcta attgcaaaga gcggcttcga
     2341 ggttggggtt cgccgcctaa tccatcataa aaggagagac catcatgaag gcattgaaag
     2401 gttcgaagac ggaaaaaagc ctgaaggacg catttgcagg ggagtcgcag gcaaatcgcc
     2461 gctaccttta tttcgcagcg aaggcagatg tggaaggaca gaacgatgtc gcgtccattt
     2521 tccgctccac cgcggaagga gaaactggtc atgcgcacgg gcacctggaa tatctggaat
     2581 cggtaggtga tccggcgacc ggcctgccga tcggtcgttc gcgagacaat ctcaaagctg
     2641 ctatcgcagg cgaaactcac gaatacaccg acatgtatcc aggcatggcc aagaacgccc
     2701 gcgatgaggg cttcgaggaa attgcggact ggtttgagac gctcgccaag gcagaacgtt
     2761 cgcatgccaa tcgattccag aaagcactgg atgcgctgac cgactgacgc cgccagttgt
     2821 tacacaaggg gagagaagac agcaaaatct tgtctccttt ttcttatgcc agttcgtatc
     2881 tatccgccta aatgacagtc cgcgaaggtt cactggaagc gccgacccgg catccgatcg
     2941 actggcagtc cgccgagttc tacgatgaag aaaaggcgca tgccgagatg catcgcattt
     3001 tcgatatctg ccacggatgc cggcggtgcg tgaatctttg cacggcgttc ccgaccctgt
     3061 tcgatctcat cgatgaaggc aagaccggcg aactagacgg cgtcgacaaa ggcgattacg
     3121 gcaaagtcgt cgacaaatgt tatttgtgcg acatgtgcta catgtccaag tgcccctacg
     3181 ttccgccgca cccctggaat gtggactttc cacatgtgat gctgcgcgcg aaagcagtca
     3241 aattcaaaaa aggcgaaacc gtactgcgcg acagactgct gaccagcacg gatgcggtag
     3301 gcaaactggc ctccatcccg gtagtggtgc aaatggtcaa tgccgccaac aaggcgcgct
     3361 ccgctcgcac gctaatgcaa tcagccttgg gcgtgcataa ggatcgggtg ctgccggaat
     3421 acaccagcgc gcattttcga aaaaccgccc ggccgcagtc atcgtttgcc gtcaaggatg
     3481 gcaaaaatgc cccgggcaag gcagccatct tttccacctg ctacgttaac tacaatgagc
     3541 ccggactcgg tcatgacctt ctgaagatcc tcgagcacaa cgaagtgccc gccatcgtgg
     3601 tgcaaaaaga ggcctgttgc ggtatgccca aactggaatt gggtgatctg gcaacggtgg
     3661 cgaagctgaa gcaggcgaat atccctgtgc tcgccaatct cgcgcgcgag ggctatgcca
     3721 tcctgaccgc tgtcccgtcc tgcacgttga tgttcaagca ggagttgccg ctgatgttcc
     3781 cgcatgatga ggatgtaaaa gcggtgcagg aagccatgtt cgatccgttt gagtatctgg
     3841 tcatgcgcaa cagagatgga ttgctgaaaa ctgattttac gacgcctctc ggcaagattt
     3901 catatcacat cccgtgtcat ttgcgcgtgc aaaacatggg gcagaagacg cgtgaattgc
     3961 tggaactcgt gccgcggacg gaaatcaata ccgtggaacg ttgtgctggc cacgacggca
     4021 cttggggcgt caagacggag ttcttcgctg attccatgaa aatcggacgc ccggtattcc
     4081 gtcaaatggc ctcaacggaa ccagactacg taagctcgga ttgcgcgata gcgggcagac
     4141 atatagccca agggatagcg gatggcggcc tcgcgcaacg tccgaccgaa aaaaaacatc
     4201 cgctgaccct gctccgaatg gcctacggca tttgaagcac gccatacgag aatccgaatg
     4261 ggccagatta cagcagaaag cctgatgtct ctggagcagt acgccaagaa ccgcaaggaa
     4321 ttccgcgcaa aggtgcttgc gcacaagaag gatcgtaccg ttcgacttgg cgagcacgtg
     4381 accctggtgt tcgaagatga gctgaccatg cgttaccaga tccaggagat gttgcgagtg
     4441 gagcgcattt tcgagcagga cggcatacgg gacgagcttg attcgtataa tccactggtg
     4501 ccggacggca gcaactggaa agcgacgatg ctaattgaat atcccgatag tgcagagcgc
     4561 cggatcgccc tttcgaagtt gatcgggatc gaagatcgag tgtgggttga agtggaaggc
     4621 caggctcgga ttttcgcaat cgcggatgaa gacatcgacc gggaaaataa tgataagacg
     4681 tccgctgtgc atttccttcg atttgaactg cctgcggtcg taaagcaggc cctgcgcgcg
     4741 ggagcgggac tttccgtcgg agtggatcat cccaactatt cggcattgat aggcaaggca
     4801 gggcccgcga cggttcgctg cctgctgaaa gatctggttt tctgaaccag ttttcgggtt
     4861 cgtcggtcta ccagtacaat aaccccgagc tatggggtgg ctgttcggta tttgaaaccg
     4921 gaatttcgag gatcgcaatg ctggaatcgc gcatgatcgg tatgcttgag aaccgcctga
     4981 agcgcgcggg gcttcctatc agcatgacgc tctggaacgg cgataccatc gagtctggcc
     5041 cgggcgcgaa tctcagcctg acggttaaat ctccccaggc tttgatgtct cttgcgaacc
     5101 cgagccttgg agggattgca cgggcctatg tcgagggtca gctcgatctg gacggtgaca
     5161 tcaggcagac catccgggcg ggcgaaggcc tggtgtctgg gcaatcgacg acctaccaga
     5221 atcggccgaa gacgtggaaa tggtggcggc atacccgtcc tgcggaccgg aaaaacgttc
     5281 agcatcatta cgacgttggg aatgatttct atggcttgtg gctagaccgc aaccgggtct
     5341 actcatgcgc ttattacaag acgccggaag atacgctcga ccaggcccaa gagcagaaac
     5401 tggagcatat ctgcctcaag ctcgatctca agcccggcga gcgattcctt gatatcggct
     5461 gtggctgggg cgggctgatt ctgtgggcaa ccaaaaacca caaagtcagc gcgctcggca
     5521 tcacattgtc cgaagaacaa catgcgtacg ccacgcaaaa aatacgggaa ttcggattgg
     5581 aaagccgttg cgaagttcgc ttgatggatt accgcgacgt ggaagaggga agtccgttcg
     5641 acaaaatagc gagcgtgggc atgttcgaac acgtcggaaa gcgcaatttg aacgcctatt
     5701 tcgccaagat ctcccgcttg ctgaagccgg gtggcatggt catgaaccat ggcatcacca
     5761 cgaattctct ggaagatgcc gagcttggca gcggcattgg cgaattcgtg gacgagtatg
     5821 tattccccgg cggcgagctg gtgcatgttt cgcgcgtact ttctgaaatg tcccagcagg
     5881 gtttggagcc ttgggatgcc gagtgcctca ggccgcatta tgcgagaacg ctttgggaat
     5941 gggtccgcag actggagtca aggcaagacg aagcccgcag aattgttggt gagaagcttt
     6001 accgcatctg gcgcatttat atggccggct ctgcccacgc attcgatcgc ggctggattt
     6061 caattttcca gatactcgga actaaatcct tgtcggacgg ttcggtgtcc tacccgttga
     6121 cgcgcaacca tgtttacaga tgaacttggt tagtagttcc agccggcaat gcggttgacc
     6181 gtttttgccg tggtgcttgt attcgcgcta gcgggatgcg ctggttccac tgctccgagc
     6241 ggaaagtcgt cgcaacgtgg catgacgaaa gatatcggtg gcgtgcccga ggaaaaggaa
     6301 ttcaaggaag tcgattccga gtttccagcg tatccccctg attccggtct tctggaattt
     6361 tatccgagac aaaaatctca gaaccgtttt tatgttgacc cgaatacgtt atcgatcggg
     6421 ccggaccggg tcattcgata tagcctcgta gtcaagtccc catccggggc gctgactgtc
     6481 agttacgaag gcctgcgctg caagacttcc gagtacaagc tgtatggatt cggcgtcacg
     6541 gccggagaat ggaccaggtc ccgcaactca gagtggcacc ccgtccccaa aacagccggc
     6601 gatttccgtt ttgcgctgta caaggactac ttctgtgacc tggaggcaat tgccggacgc
     6661 aacgaaagag accttattgc caacctggcg ggcaatccac tcaacaacgt taccgacaag
     6721 aatcgatgat tacctgtaaa gcggcatagc tcactggtgt gcgccgtaat cgtaacgtgg
     6781 gtctatattt caaagcagga ccagattatc gcgatggatc agttcgggtt cgtcgatata
     6841 gcctagccgg gcttcaattt catgactggg acgcttcagg attttacgcg tctccacaga
     6901 gctgtaattg accaggccgc gtgcgatttc acaatcgcgt gagtccaggc aactcaccat
     6961 ttcaccgcgc tcgaaatcgc cggccgcttc tacaaccccg attggcagca ggcttttgcc
     7021 ttcggaccga agcgctcgta ccgcgccatc gtcgagtttg agtttgccac gcacctgaag
     7081 atgatccgcc atccattgct tgcgtgcagc gagcgtcatg gtttccgcga ccagttgcgt
     7141 accgatgcgt tcgccggaag ccagtctgat taatacgtcg ggctcacgtc cggaggcgat
     7201 gacggtcgat gcgccgctac gcgcagcgcg tttggcagcc agaactttgg tgagcattcc
     7261 cccgctgccg aggtggctgc cgatgccgcc cgccattttc tccagttgcg catctcctgc
     7321 ccgcgcatca gtgacaagtg tcgcatcgtt attgcgacga ggatctttgg tataaagacc
     7381 tggctggtcg gtcagaatca gcagcgcatc ggcttcgatc aggtttgtaa ccagcgctgc
     7441 taatgtgtca ttgtccccga accggatttc atcggtggcg accgtatcgt tttcgttgat
     7501 gacaggaatg acgccgaggt cgagcaacgt tcgcaaagtc gaacgggcgt taaggtagcg
     7561 tttgcggtct gcgagatctt catgggtcag caggacctgt gcggtatgca gcccatgttt
     7621 gcgaaagcag gattcgtaag cttccaccag gcccatctga ccgaccgccg ccgctgcctg
     7681 caattcataa agtgcatttg gccgccgctt ccatcccagg cgctgcattc ccgctgccac
     7741 ggcaccgctg gataccagaa ccacttcttt cttgcgtcgg gtcaattcgg cgatttgctc
     7801 ggcccagcgt gcgagggcgg catggtcgag tccctgtcct tcgttcgtga ccagactgct
     7861 accaaccttg acgacaaggc gcaccgcatt tgcggcgacg gatttcatgg tgcgggtctg
     7921 tcttcggtgc ggccgtccgc gatttccgtt tcggcgtctg gcctgcgata gcgttccaga
     7981 tgttccatga tcgcaaacgt caattcgcgt cctccttcac cagtgagcgc cgaaatgata
     8041 aacgttttct caggttcgga aaccaggcgc gcataagctg acagaaacgc tttgattctg
     8101 ctgtcacggt cttgtgtttc aatgaggtct accttgttca ataccagcca gcgcggcttc
     8161 tggaatagcg cctcatcgta tttgcgcagc tcttcgacga ttgcgcgcgc gtcttttgcc
     8221 ggatcggcgt ccacatcgaa aggcgccaga tcgacaaggt gcaatagcag ccgggttcgt
     8281 tgcagatgac gcaaaaactg atggccaagc cccgcgccct cggaagcacc ctcaatcagt
     8341 ccgggtatgt ctgcaatcac aaagctgcgg ttttcgtcca cacgcacgac gccgagattc
     8401 ggatgcagcg tggtaaaggg atagtccgcg accttgggtc gggccgcaga gaccgagcgg
     8461 atatacgtgg attttccggc gttgggcatg ccgagcaagc cgacatccgc cagaaccttc
     8521 agctcgagtt tcaaacggcg gttctctcca agcgtcccgg gcgtgaattg gcggggtgag
     8581 cgattggtgc tggacttgaa atggatgttg ccaaggcctc cttgaccgcc tttggccagc
     8641 acagcggttt gaccgtgaac agacaggtcc gcgagcactt cgccggactc caggtcggtg
     8701 acgacggtac caaccggaaa acgcaaaatg acatcttccg ctgccttgcc gtagcaatcc
     8761 gacccacggc ccttttcgcc gttacgtgcg cgatgaatgc gggcataccg aaactcgacc
     8821 agcgtgttga tgttgcggtc ggctacggcg agaatggaac cgccacgacc cccatcgccg
     8881 ccgtcgggac cgcctctcgg gatgaacttt tcccgccgga aactggcgct tccatcgccc
     8941 cctttcccgg catgcacttc gatgatggct tcgtcgatga atttcatgat caaaccaatt
     9001 tagccacagt ggttcgtttt caacagcgct ttctcgggag agcatcccga tcggggaaca
     9061 cgatcgtgtg gcgaccgctc aaccgggaat aaaaaagccc taccggatcc gatagggctt
     9121 cttgctggca gcggaaaact caggccggta gaacgctgac agtctttttc ttgaacgggc
     9181 ccttggtaac aaaagtcacc gtcccggtga ttttggcgaa caaggtgtga tccttaccga
     9241 tcccgacatt ctctcctgga tgcacctgtg tgccgcgctg gcgaacgatg atgctgcctg
     9301 ccgggattac ttcaccgcca taacgcttca cgcccagccg ctttgattga gagtcacgtc
     9361 cgttgcggga actgcctcct gcttttttgt gtgccatatt tagtttcctt gtgccgacat
     9421 cgcgccgatc tcgatctcgg tgtaattctg gcggtgccca ccgtggcgct gataatgttt
     9481 gcgtctgcgc attttgaaga tatggacttt gtcgtgccga ccgtgggaca ccactgttgc
     9541 ttgaacggat gcgcccgcca ggacgggagt cccgatcgat actttctcac catcggcgat
     9601 catcagcacg tccagcgcca cttggctgcc gatatcagca ggtaattgtt ccacttttat
     9661 tttttcgcct ggagacaccc ggtattgctt gccgccggtt ttgatgaccg catacatgat
     9721 ttggagccct cggtagcctt ttgaggaaag ccgggcatac tacaaaaaaa gaaaggctta
     9781 gtaaagccaa attggaaaag ccggcacggc cggaattttg cgcgaggctg cttgggtttc
     9841 cggctggttt ccttgacacg gccccgacac gcttcctacc atcgtctgtt ttgactttcg
     9901 catggtattc attcgcaatt gggtcctgaa atcatcaaca gccttatcgc cgaagacctg
     9961 aagcaggtcg atgccgtcat tagccatcgc cttagctctg acgtcgtgct aatccggcaa
    10021 gtggctgact acatcatagc gagcggcggg aagcgactgc gcccggcgct gctcatcttg
    10081 tctgcgagag cgtgtggtta tcagggaaca catcatcacg agcttgcagc ggtcgtcgaa
    10141 ttcattcaca cggctacatt actgcacgat gatgtcgttg acgattccca catgcgccgt
    10201 ggacgcgcga ccgcgaattc gctgttcggc aatgccgcgg cggtgctggt tggtgatttt
    10261 ctttattccc gcgcctttca aatgatggtc ggcgtgcgaa gcatgcgcat catggaagtg
    10321 ctggccgatg caaccaacaa catcgccgaa ggcgaagtca tgcagttgct gcatgttcgc
    10381 aatgccaacg taaacgagca ggattacctg cgggtgattc gtgcaaaaac tgccaaattg
    10441 ttcgaagcgg ccggccggct gggtgccatt ctggccgccg tcccgccggc acaagagaac
    10501 gcgctggcaa gctatgggat gcatttgggc acggcgttcc agctggtgga tgatatcctc
    10561 gactattccg gtgatctcat gcagaccggc aaaaatctcg gcgacgatct tgcagagggc
    10621 aagactactc tgcccataat tcatgcgatg cgccacggca gttcggagca ggaaacgctc
    10681 attcgcaatg cgctggtcag cggtggttta agcgaattcg aacccgtcat ggaagccatg
    10741 cgcgagaccg gggcgctcga ttacactcgc atgcaggccg aggcttccgc acgccacgcg
    10801 cgcgactcaa tcgcggtgct tactgattca aagtacaagg atagtttgct agaattggcg
    10861 acctttgcgg tgacccgcaa gtactgaaac tcaccgtcgg ggtgtagctc agcctggtag
    10921 agtactgcgt tcgggacgca ggagtcggag gttcgaatcc tctcaccccg accattccaa
    10981 cgtctgcgtg cgaggttcgc gtggcaaaat tcctcctcgt catcgctgta ttcatcgtgg
    11041 tttacctgct tttgcggaat gtcgcaaaca agccaggtgc ggcttctgca cgggacaccg
    11101 cttccaatgg cagcgccgcg aaaaagggtg catcgaaact cgccgaagat atggttcgtt
    11161 gcggaatttg cggtgtgcat ctccccacca gcgaggctat cacttcccgg ggcgacttct
    11221 tttgcagcaa agaacacttg cagctagccg tccggcaacg cgacagccat tagccgccgt
    11281 cgccggccga tgagcagtat tcctttttca cccaagactt tctccgtgga ccgtcggcag
    11341 tacggcgacg cgttctggcg atcgctgttc tatttcaacg tttatcgttt ggctgcggcg
    11401 ctggttttgc tggctgccgt cctggccctc ggcggtgaag tcccgttcgg ctccaacagc
    11461 cggcatgcct atctttacag caacgtctgc tacatcgttt tcagcgtaat ggcatttggc
    11521 gccgtactgt cgcgcaagcc ttatttcacc ggccagcttg cggtacagat atgcgcggac
    11581 atcttgttcc tgacgacgat gatgcatttc agcggcggca tcagcagcgg cctgggcctg
    11641 cttctgctgg tctcgcttgc ggcggttgga ctgatcagtc gcggccgtct cacgcttttc
    11701 tttgcggcac tggcaaccat tgcgatactt ggagaacaca cttatgaagt agtgttcctc
    11761 aacggttcca tttcacagta tgcccaggct ggcttgctga gcattggctt ttttgcaacc
    11821 ggctggctgg cgcattcact tgccaagcgc gcagttgcca ttgaggccat cgcggcgcag
    11881 cgcgaaatcg atctcgccaa tctggcgcag gtgaatcagt tggtgattca ggatatgcag
    11941 gatggcgtgc tggtggttga tgagcgcggc gtgattcgcc agatcaacag ccgcgccgag
    12001 gcgatccttg gtccgttgcg ccgttccaac cgggaagttc tattgcagga ctatgcgcct
    12061 accgtcgcgg cccggctgga acaatggcgg cgcgatcccg gtgccggatt cgatccgcta
    12121 cgcaccgtac agacgaacaa ac
//
LOCUS       DATMZQ010000335         3987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2211684, whole genome shotgun sequence.
ACCESSION   DATMZQ010000335 DATMZQ010000000
VERSION     DATMZQ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211684"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VN496_10670"
     CDS             <1..712
                     /locus_tag="VN496_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF17459.1"
                     /translation="PEERLAAWRAVLVCPTASVRSQSKQHRPQDIVFPQQITEAVWRC
                     GFNARSSFGAHSYFAARAGGNLLVDSPRYSAELVKWFENAGGLAHILLSHQDDVADAD
                     QYAKQFGARVWIHREDRSVAPYATDLLEGVSTRVIASGVVAIPVPGHTRGSVVYLLDD
                     RVLFAGDSLAWSAREQDLVAFRDACWYSWSELTASLGKLLDYSFEWLLPGHGWPVHLA
                     RHEMNARLRALIGRMREA"
     gene            760..2319
                     /locus_tag="VN496_10675"
     CDS             760..2319
                     /locus_tag="VN496_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXF17460.1"
                     /translation="MRELEQLLLLFGAAVILAAAARRVGAPYPVFLALGGALLAFVPG
                     APSITFPPELALALFVAPVLLDAAYDASLRDLRENWGPVTGLVVFAVGFTAAAVAVVA
                     HTLVPSLPWAAAIALGAVVAPPDAVAATAVLRQLRPPQRLLTILEGESLLNDASALLI
                     YRLAIGAVAAGSFSIAAVAPVFLIAVVGSVAAGLALGWLVFRLIDRVRHVPTAIILQF
                     ISTFSVWLFAENIGLSGVLTTVCYAITLARTAPERTPASIRIPSYAVWDTVIFTLNIL
                     AFIFIGLQIRPIFESLQPSDRFGYFIVAGAVLLTVIVVRFAWHMPFNAVIRWRQRRSG
                     FHPPRPMLRPTIGSGLVISWAGMRGIVTLAAALALPAAFPYRDLIVLTAFSVVLGTLT
                     IQGLTLKPLLRALDLHDNDPIGAEFSAARERALQAALNSLDGNRSPLAEFIRHEFTSR
                     LANEQRDADSRDAAGSAHSELYRGAVRTARQAVIAMRDNEEIGDDAFHRIEQELDWLE
                     MAGSRNGPEKN"
     gene            complement(2339..3097)
                     /locus_tag="VN496_10680"
     CDS             complement(2339..3097)
                     /locus_tag="VN496_10680"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXF17461.1"
                     /translation="MHVAREIQQVREWIDGARKVVVLTGAGISTESGIPDFRGPQGVW
                     TKNPQAEKLSNIHYYLSDAEVRKESWKARLAHPAWTAQPNDGHRALAALERRGKLHAL
                     ITQNIDELHQMSGNSPETVIEVHGTVRKVVCMVCGMTAPMQRALDRVRAGEPDPPCRD
                     CGGILKSATISFGQALVPEVIARAMHAAAEADLFLSVGTSLQVYPIAGAVEVAQAAGA
                     KIVIVNAEPTPFDETADAVFREPISTVLPRMLQP"
     gene            complement(3153..3698)
                     /locus_tag="VN496_10685"
     CDS             complement(3153..3698)
                     /locus_tag="VN496_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17462.1"
                     /translation="MRQLLAGILLLIGSAASFAAETDTNGSGGVPGLSHSLTVELQNF
                     LRQRNIDVDQLSAGEMVSVMVDWYRLTPVKKVQGDVAGDALVYRYGGWSEGCATAFKL
                     SLLRRVTVRDASGADDERLAGITMMFEPSAQAELMPFSAVASDANSIGVFVTAVESSP
                     AFQLLAGATPMGVMVESGGVR"
BASE COUNT          710 a         1242 c         1192 g          843 t
ORIGIN      
        1 gccggaagag cggcttgcgg catggcgagc ggtactcgtg tgtccgacag cgtccgtacg
       61 cagccaaagt aaacaacacc ggccgcaaga catcgtcttt ccgcagcaga tcactgaggc
      121 cgtgtggcgg tgcgggttca acgcaaggtc gtctttcggc gcgcactcat acttcgccgc
      181 gcgcgcaggc ggcaacctgc tggtagactc gccgcgctat tctgcggagc tcgtgaagtg
      241 gttcgagaac gccggcggcc tcgcgcatat cctcttatcg catcaggacg acgtcgcgga
      301 cgccgaccaa tacgcaaagc agtttggcgc gcgcgtctgg atccatcgcg aggaccgctc
      361 ggtggcaccc tatgcaaccg atcttctgga aggcgtctcc acgcgcgtta tcgcgtccgg
      421 tgtcgtcgcg attccagtgc cgggacatac acgcggcagc gtcgtgtacc tgctggacga
      481 tcgcgtgctg ttcgccggtg actcgcttgc ctggagcgca cgcgaacagg atctcgtcgc
      541 atttcgcgat gcgtgttggt attcctggtc tgagctcacc gcatcgctcg gcaagcttct
      601 cgactacagc ttcgagtggc tgctgcctgg ccacggttgg ccggtgcact tggccaggca
      661 cgagatgaat gcgcggttgc gtgccttgat cgggcgcatg cgagaggcct agcccggccc
      721 aattgtccgc tggttccgca tgtgacctaa tatcgtcata tgcgcgagct cgaacaactc
      781 ctgctgctgt tcggcgcggc cgtgatcctc gctgctgcag cacgccgtgt cggagcgcct
      841 tatccggtat ttctggcgct cggcggtgcg ctgctcgcat tcgttccagg cgcaccctcg
      901 attactttcc cgcccgagct tgcattggcg ctcttcgtcg cgcccgtatt gcttgatgcg
      961 gcttacgacg cgtccctcag ggatctcagg gagaactggg ggccggtcac cggactggtc
     1021 gtatttgcgg ttgggttcac tgctgcagcg gtcgcggttg tggctcacac gctggtgccg
     1081 tcgttgccat gggcagcggc gattgcgctc ggcgcagtcg tcgctccacc cgatgccgtt
     1141 gcagcaacgg cagtgctgcg gcaattgcgg ccgccgcagc gcctgctgac catactcgaa
     1201 ggcgagagcc tgctgaatga cgcgagtgca ctgttaatct accggctcgc cattggagct
     1261 gtagccgccg gaagtttctc catcgctgca gtggcgccgg tcttcctgat cgccgtggtc
     1321 ggcagtgtag cggcaggact ggcactcggc tggctcgtct tccggttgat cgatcgcgtg
     1381 cggcacgtac cgactgcgat catcctgcaa ttcatcagca cgtttagcgt gtggctgttc
     1441 gccgagaaca tcggactctc aggcgtgctg accaccgtgt gctacgccat taccctggcc
     1501 cgcacggcgc cggagcggac gcccgcttcg attcgcatac cgtcgtacgc cgtgtgggat
     1561 acagtcattt ttacgttgaa catcctggca ttcatcttca tcggcctgca gatccggcca
     1621 atatttgaaa gcctgcaacc atcggaccgt ttcggatatt tcatcgtcgc gggcgcggtt
     1681 ttgcttacag tcatcgttgt acgctttgcc tggcacatgc ctttcaatgc agtcatcagg
     1741 tggcgccagc gccgcagcgg ttttcatccg ccgcggccga tgttaaggcc aacgatcgga
     1801 agtggactgg tcatctcatg ggcgggcatg cgcggcatcg tcactctggc agcagcactg
     1861 gcgctgcctg ccgcatttcc ttatcgcgac ctgatcgtgc tgacggcgtt ctcggtggtg
     1921 cttggcacgc tgacgatcca gggtttgacg ctcaagccgc ttctacgtgc gctcgacctg
     1981 catgacaacg atccaatcgg cgctgaattc agtgcggcgc gtgaacgcgc cctacaggca
     2041 gcgctcaaca gtctggatgg caaccgatcg ccgctcgcgg agttcatccg ccacgagttc
     2101 acctcgcgac tggcaaatga acaacgcgac gcggactcga gggacgccgc tggttccgca
     2161 cacagcgaac tctatcgcgg cgcggttcgg acggcgcggc aagccgttat cgcgatgcgt
     2221 gacaacgaag agatcggaga cgatgccttt catcgcatcg aacaggaatt ggactggctc
     2281 gagatggcag gcagcagaaa tgggccggaa aagaactgaa ctcgaaagtc agacgtactc
     2341 agggctgcag cattcttggt agtacggtgc tgatcggctc gcgaaacacg gcatcggcgg
     2401 tctcgtcgaa gggcgtcggt tcggcgttca cgatcacgat cttcgcgccc gctgcttgcg
     2461 caacttcaac tgcgccggcg atcgggtaga cctgcaggct ggtaccgact gacaagaaca
     2521 ggtccgcttc cgcggcggca tgcatcgcac gtgcgattac ctcggggacc agcgcttgac
     2581 caaaggaaat ggtggcgctc ttcaggattc cgccgcagtc cctgcagggt ggatccggtt
     2641 cccccgccct gacgcgatcc agtgcccttt gcatcggtgc tgtcataccg cacaccatgc
     2701 agacaacttt acgcactgtg ccgtggactt cgataactgt ttctggtgag tttccggaca
     2761 tctgatgcag ctcatcgatg ttctgcgtga tgagtgcatg cagcttgccg cggcgttcca
     2821 gcgctgcgag tgcgcggtgt ccatcgttcg gttgtgcagt ccaggcgggg tgcgcgagcc
     2881 gcgccttcca ggattccttg cgcacctcgg cgtcggacag gtagtagtgg atgttggaga
     2941 gcttctcggc ctgcggattt ttggtccata ccccctgagg cccgcgaaaa tccggaatgc
     3001 cggattcagt ggagatgcct gcgccggtca gcacgacaac ttttcgtgcg ccatcgatcc
     3061 actcccggac ttgctgtatt tccctggcaa cgtgcatgca ggacctgcta aatggttacc
     3121 gctgtggacg cgggaataca gctccgggct gcctaacgca ccccgccgct ttcgaccatc
     3181 acgcccatcg gcgtggcgcc cgcgagcagc tggaaggccg gtgagctctc gacagcggtg
     3241 acgaaaacac caatcgaatt cgcatcggaa gcgaccgcgc tgaacggcat caattccgcc
     3301 tgggcgctcg gctcgaacat catcgtgatg ccggcgaggc gttcgtcatc cgcgccgctt
     3361 gcatccctta cggtgacccg acgcaacaag ctcaacttga atgcggttgc gcagccttca
     3421 gaccagccgc catagcgata caccaatgcg tcgccggcca catcgccttg cactttcttc
     3481 accggcgtaa gccgatacca gtcgaccatc acgctaacca tttcgccggc gctcagttga
     3541 tcaacatcga tgttgcgctg gcgcagaaaa ttctgcaatt ccacggtcag actgtgcgaa
     3601 agacctggca ccccgccgct gccgttggta tcagtctccg cggcgaaact cgcagccgac
     3661 ccgatcagca gcaaaatgcc agccagtaac tgcctcatgt tcttccgccg cctccacgct
     3721 tgttgcacgc agaccagttt aagcatttat gcgggcttct gcctacaagc ttctcgtcgg
     3781 attggtagcg tgcttttgca ttgcttgttt catgctctgg cacggaattg aaaagcgagc
     3841 cagaaccggc gtcgtgaagc tcaattccga aactgcacga cgttggatta catttccgac
     3901 cggacgcgat cagaacatcg caagaaagat gaccagcggt ctgcaaggat tagcgtcgat
     3961 ccctcgtccg cctccgcggc ggagacg
//
LOCUS       DATMZQ010000336         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2692823, whole genome shotgun sequence.
ACCESSION   DATMZQ010000336 DATMZQ010000000
VERSION     DATMZQ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692823"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VN496_10690"
     CDS             <1..278
                     /locus_tag="VN496_10690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HXF17463.1"
                     /translation="SVKPFLDDDEHGIFATRAPKRPNPIGLSVVKLVARNEASLDIEN
                     VDILDGTPLLDLKPYLPQFDAPDNVRIGWFGGRLGRISQARSDDRFG"
     gene            complement(356..739)
                     /gene="crcB"
                     /locus_tag="VN496_10695"
     CDS             complement(356..739)
                     /gene="crcB"
                     /locus_tag="VN496_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXF17464.1"
                     /translation="MGGSGFLAVGVGAAVGAWLRWWLGVALNAVNPNLPYGTLAANLI
                     GGYLVGVAVEYFSQQGGVPLELRLLVITGFLGGLTTFSSFSAEAVGLLMQSRYGWAIA
                     LITTHLFGSILMTILGIFSVRAVSM"
     gene            complement(784..1155)
                     /locus_tag="VN496_10700"
     CDS             complement(784..1155)
                     /locus_tag="VN496_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17465.1"
                     /translation="MRNIILLCMALAMGACANAPRLSTDQQFEAYRMQLRGERDTGKI
                     TVVEEQEKLRDHFWQVYGKDPNSIGHFAFSVSLMRSVQAGSFPLEDADALIAAKEAEI
                     FAMKMASRQRQADCTYDCPWN"
     gene            complement(1215..>2688)
                     /locus_tag="VN496_10705"
     CDS             complement(1215..>2688)
                     /locus_tag="VN496_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF17466.1"
                     /translation="AGTRIEDSGVIFTRVEDEIRRTIDPTDLDVVIDNIGIPPSTNLA
                     YSDNVTLSSGDGEILVSLKPEHKTATKEYIRRLRQVLPNQFPECTFYFQPGDMMNQIL
                     NFGLPAPIDVKIIGGDYKNFQLAKQIEARMKRIPGAVDVHVHQIMNQPALRVEVDRTR
                     AAQLGVSQREIAENFLIASSSSVVVTPNYWNDPKTGRPYQVVVVQPHETALNSVEAVL
                     NIPLSGKGQSPTQTLGNVATVKHVELPAIINHVNTELAFDVYANVQGRDLGAVASDVR
                     KVVDEFIPKAREQKNNTRIEIAGQAESMYDAFTRMGIGLIAAVLLVYFIMVINFQSWT
                     DPFIIITALPGAFCGIIWMLFLTGTSFSVPSLMGAIMSVGVATANSILMVSFANEQLR
                     NGMSPIDAALEAGSTRLRPVLMTAAAMVIGMAPMASGLGEGGEQNAPLGRAVIGGLIL
                     ATVATLFFVPVVFSLLRRHGVPKPKFADEEGGLATEGVRR"
BASE COUNT          590 a          814 c          747 g          537 t
ORIGIN      
        1 tgtcggtcaa gccatttctc gatgatgatg aacacggcat attcgcaacc cgcgcaccga
       61 agcggccgaa tccgatcgga ttgtccgtgg tcaaactggt cgcgcggaac gaagccagtc
      121 tcgatattga aaacgtggac atcctggacg gcacgccgct actcgacctc aagccttacc
      181 tgccgcaatt cgacgcgccc gataacgtgc gtatcggctg gttcggcggc cggctaggcc
      241 gaataagtca agctcgttca gacgaccggt tcggctgatc tgtgattcga ccgcgcgcta
      301 gtgcagctcg aagtatccgg ctgccggccg caagtcaaag caggacttgg tgggcttaca
      361 tgctcaccgc gcgcacggag aaaataccaa ggatggtcat gaggatcgaa ccgaatagat
      421 gggtcgtgat caaagcgatg gcccatccat agcgactctg catgagcaat ccgaccgctt
      481 cggccgagaa ggaagaaaag gtcgtcagcc ctcccaaaaa gccggtgatg accagaagac
      541 gcagttccag cggaactccc ccctgctggg agaaatactc gacggcgact cccaccagat
      601 acccgccgat cagattggcg gccagcgtgc cataaggcag attgggattg actgcgttaa
      661 gcgccacccc cagccaccag cgcagccatg cgccaaccgc ggcgccgact ccgacggcca
      721 aaaaaccgga tcctcccatt tgccattccc caggtcctca aatggaagat gataatccga
      781 gactcaattc cacgggcaat cgtatgtgca gtccgcctga cgctggcgcg atgccatttt
      841 catcgcgaag atttcggcct ccttcgcagc tatcaaagca tcggcatcct ccagcgggaa
      901 actgccggcc tggacgctac gcatcagcga aacggaaaaa gcgaaatgac cgatgctgtt
      961 ggggtccttg ccatacacct gccaaaagtg atcacgcagt ttttcctgct cttccaccac
     1021 ggtaatcttg cccgtatcgc gctctccccg tagctgcatc cgatacgctt cgaattgctg
     1081 atcggtagaa agacggggag cgtttgcaca cgcgcccatt gccaatgcca tgcacagcag
     1141 aatgatgttt ctcatgattg ttcccttgtg aaaacgattg cagattgaaa aaacgacagc
     1201 acaccccaaa ttccttatcg ccggaccccc tcggtcgcga gacctccctc ttcatccgca
     1261 aacttcggct tcggcacgcc atggcgccgc aacaggctga aaacgactgg cacaaagaac
     1321 agcgtggcca ccgttgccag aatcagaccc ccgataacgg ctctgcccaa cggtgcgttc
     1381 tgctcgccgc cttcgccgag cccactggcc atcggcgcca tgccgatcac catggcggct
     1441 gcggtcatca gcacgggacg cagccgcgtc gagccggctt cgagcgcagc gtcgatcggg
     1501 ctcattccgt tgcgcaactg ctcgttggcg aacgacacca tcaagatgct gttcgcagta
     1561 gccacgccga cactcatgat cgcgcccatc agggacggca cgctgaaact ggttcccgtc
     1621 aggaacagca tccagataat tccgcagaat gcaccgggca gcgcggtgat gatgatgaat
     1681 ggatcggtcc aggactggaa attgatgacc atgatgaaat acaccagcag cacggcggca
     1741 atcaggccga tgcccatgcg cgtgaaagcg tcgtacatgc tttccgcctg gccggcgatt
     1801 tcaatgcgcg tgttgttctt ctgctcgcgg gccttgggaa tgaattcatc caccactttg
     1861 cggacatcgg acgccaccgc accgagatcc ctgccctgca cgttggcata cacatcgaac
     1921 gcgagttcgg tgttgacgtg attgatgatc gcgggcagtt ccacatgctt gaccgtcgcg
     1981 acgttgccca acgtctgcgt tggcgactgg cctttgccgg acagcgggat gttgagcact
     2041 gcttcgactg aattgagtgc cgtctcatgc ggctgaacca cgacgacctg atagggccgg
     2101 ccggtcttcg ggtcgttcca atagttcggc gtaacgacaa cgctggacga cgaggcgatc
     2161 aggaagtttt ccgcgatctc gcgttgtgag acgccgagtt gcgccgcgcg cgtgcgatcc
     2221 acttcgacgc gcaaagccgg ttggttcatg atctgatgca cgtgcacatc aacggcgcca
     2281 gggatacgct tcatgcgcgc ttcgatctgc ttcgccagct ggaaattctt gtagtcgccg
     2341 ccgatgatct tgacgtcgat cggggccggc aacccgaaat tgagaatctg gttcatcata
     2401 tcgcccggct ggaagtaaaa agtgcactcc gggaattgat tcggcaacac ctggcgcagc
     2461 cggcgtatgt attctttcgt agcggtcttg tgttccggct ttagcgagac caggatttca
     2521 ccgtcgcccg aagacaaggt gacattgtcg ctgtacgcaa gatttgtgga cggaggaata
     2581 ccgatgttgt caatgaccac gtccaggtct gtggggtcga tcgtacggcg gatctcgtcc
     2641 tctacccggg tgaaaatcac gccggaatcc tctattcggg taccggcc
//
LOCUS       DATMZQ010000337         6689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3040593, whole genome shotgun sequence.
ACCESSION   DATMZQ010000337 DATMZQ010000000
VERSION     DATMZQ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040593"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VN496_10710"
     CDS             complement(<1..427)
                     /locus_tag="VN496_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXF17467.1"
                     /translation="MTALLDEKYAGQMCGTVRPLRETMTSTALAYESIRSLADRIASG
                     RLSPVRVAEELLQRIDVLDGRLRSFIRVTPERALAQARAAESAMKGGASLGILHGIPY
                     AAKDLFDVKGFPTTAGTRLLANSIAGQDSAAVQKLQSAGM"
     gene            complement(589..1134)
                     /locus_tag="VN496_10715"
     CDS             complement(589..1134)
                     /locus_tag="VN496_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXF17468.1"
                     /translation="MTRDAVPLKLAVPVNHTDHIEGPETARVTVVQYGDFECPMCGQA
                     FPAVKMLLNRFAQRMRFVFRHFPLAESHPNAERAAEAAEAAGAQHKFWPMHDLLFQYQ
                     SHLKPKDLHRYAEQLELDLVRFDFELSDQVYRQRVNEHVDSGKRSGVRSMPAFFVNGI
                     FVDVSFGLDHLHDAIHAKLQA"
     gene            1693..2703
                     /locus_tag="VN496_10720"
     CDS             1693..2703
                     /locus_tag="VN496_10720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXF17469.1"
                     /translation="MIPFSILDLSPIPKGANAAVALRNTLELAQHAEKLGYRRYWLAE
                     HHNMPGIASAATSIVIAHVAGGTRTIRVGSGGIMLPNHSPLVVAEQFGTLASLYPGRI
                     DLGLGRAPGTDMLTARALRRDMEDSVEQFPQDVQELQRYFADSTERQSIRAVPGAGLN
                     VPIWLLGSSLFSAQLAAMLGLPFAFASHFAPDLMRQAVEIYRNRFQPSDQLDRPYVML
                     AANTLAADTDAQARYQFTSQQQSFINLRRGAPGQVPPPIDDIDAYWSPAEKAMVERSL
                     AISFVGSIASVERGLRTFTDQLKPDELMITAHIFDQAARLRSLELVAGVRERLNITQT
                     ES"
     gene            complement(2717..3457)
                     /locus_tag="VN496_10725"
     CDS             complement(2717..3457)
                     /locus_tag="VN496_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17470.1"
                     /translation="MGRKAAIVVGVGAELGLGAALCRRFAEGGHHLFVAGRSSDKIEQ
                     VARAIVSKGGSAEAFVMDATREQDVIRLFDHAMAPRADLDALDLVAYNAGNNRKQDFR
                     ELPAETFEEFWRIGCFGGFLVGREAARRLVPLGSGTVLFTGASGSLRGKPGFAQFAAA
                     KAGLRMLAQSMAREFGPQGIHVAHVVIDGGIDGERLRRNRPEVIQQRGQDGLLSVDAI
                     AETYWQLHCQARSAWTQEIDLRPYKESF"
     gene            complement(3500..4912)
                     /locus_tag="VN496_10730"
     CDS             complement(3500..4912)
                     /locus_tag="VN496_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXF17471.1"
                     /translation="MSVPASAALDVDVRRAALNPRTRLLLEGRIVPTLLRMAWPNILV
                     MGAQAATGLIETWFVSRLGIDALAGMALVFPGFMMMQMLSAGAVGGGISSAIARALGS
                     ARRDDANALVMHAIVVNVALGLLFSVLFIAFGPQLYRAMGGEAASLQAALDYSNVVFA
                     GAVLVWLLNALASALRGTGNMLVPSIAICIGVILLIPLSPLLIFGLGPIPALGIAGGG
                     IAVLLTTALTAAGMAWYIWAGRSVVQPVLVRLRKVLFMDILRVGAIGSINTLQTTLIF
                     ALATALVGAAGGPDAVAGYGTAARLEYLLIPLVFGLGAPLVALVGTNIGAGQQSRALR
                     IALTGGAIAFALTEAIGLAAALWPHAWLELFGHETRMLETGSAYLHIVGPAFGFFGLG
                     LSLYFASQGAGRLLWPLIGSFVRLVIAIGGGWLVLRWTGSVDWLFAAMAFALVVYGIL
                     ITRTIAAGAWFGRDSSKLSR"
     gene            complement(4909..5466)
                     /locus_tag="VN496_10735"
     CDS             complement(4909..5466)
                     /locus_tag="VN496_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXF17472.1"
                     /translation="MKHKSFGKMQCPVARSLERVGEWWSMLIIRDALHGLTRFDQFQE
                     SLGIAPNMLTRRLGALVKAGLLEKRRYSERPPRDEYVLTERGRDFQPVIVAMQAWGNK
                     YFAPEGASVLLVHAKTGAPADPVLVDRKTGRPLAGPEYVIAPGPAAGEATRRKLESSA
                     WGRDTVAAPSSPFDRHRIRKSRGRL"
     gene            5586..6629
                     /locus_tag="VN496_10740"
     CDS             5586..6629
                     /locus_tag="VN496_10740"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXF17473.1"
                     /translation="MITRLTDLLGIELPIIQAPMAGAQASAMAVAVSNAGGLGSIGCA
                     TLGSEALRKELTALKAQTSRPFNVNFFCHVPPVEDAVRESAWRDKLAPYYQKYGIAAG
                     SIPAGPGRAPFTAEAADLLEDIRPAVVSFHFGLPATDLLARVKSLGAKILGCATTLKE
                     ALWLEARGIDAIIAQGVEAGGHRGNFLSDDLTTQVGTFALVPQIVRTVKLPVIAAGGI
                     ADAQGVRAVMALGAAAAQMGTAYLLCPEATISAIHRAALKSEAARHTALTNIFTGRPA
                     RGIMNYAMKELGPINHAAPAFPLATSSIAPLRAKAESAGSGDFTPLWSGQNATGCKEV
                     PAAQLTRELAGGL"
BASE COUNT         1363 a         2075 c         1999 g         1252 t
ORIGIN      
        1 ccatgccggc ggactggagt ttttgcacgg ctgccgagtc ctgcccggca atgctgttgg
       61 ccagcagacg cgtgcctgcc gtcgtgggaa aacctttcac gtcgaacaga tctttcgctg
      121 cgtagggaat cccgtgcagg attccaagac tcgcgccgcc cttcatcgcg ctttcggccg
      181 cacgcgcctg cgccaacgcg cgttcgggcg ttacgcgaat gaaggatcgc aggcgcccgt
      241 cgagcacatc gatccgctgg agaagttcct cggcgactcg tactggactc aggcgaccgc
      301 tggcaatgcg atccgccagg ctcctgatcg actcgtatgc aagtgcggta ctggtcatgg
      361 tctccctcaa gggtcgcact gtaccgcaca tctgtccggc gtacttttcg tcaagcaatg
      421 cggtcataaa aagcagaacc ctgttgctgg agatgtccgc aggttcttcg agtgcgcgat
      481 cccgcgcgcg acacgatggc gggcggggtg ggacgcggta ctcgtcacca gaaaatgggt
      541 tcgcgggact cgcagcaaac tggccgcgcg gaatccgtgc ttgcctggct aagcttgcag
      601 cttcgcgtga attgcgtcgt gcagatggtc gagtccgaac gagacatcga cgaatatgcc
      661 attgacaaaa aaggccggca tgctgcgtac gccgctgcgc ttgccggaat caacgtgctc
      721 attgacgcgt tgacggtaca cctggtctga gagttcgaag tcgaaacgca ccaggtccag
      781 ctcgagttgc tccgcgtagc gatgcaagtc cttcggtttg aggtgtgact gatactggaa
      841 cagcaggtca tgcatcggcc agaatttatg ctgggcgccg gcggcctcgg cggcctcggc
      901 tgcccgttcg gcgtttggat gcgactccgc caggggaaag tggcggaata cgaagcgcat
      961 gcgttgtgcg aagcggttca gcagcatctt cactgccggg aaagcctgac cgcacatcgg
     1021 acattcgaag tctccgtatt gcacgaccgt gacccgggcc gtttcaggcc cttcgatatg
     1081 atccgtatgg ttcacgggga ctgcaagctt caggggaacg gcgtctcggg tcatcctatc
     1141 ctcacgctga aatgtgaaac tagttggcca taatagggca cagtccgcca ccgcaactgt
     1201 tcagcagaat tggtcttgcg gtgtgcgcgc gaagttccat tgtgacgcag agcgcgtgcc
     1261 ggtgggcgcg atgcctgaaa tgcgatctgc aatcgcgcac ttcacagcga atggggggtt
     1321 ggattaccat gcgccgtcgc ccgttgcctg ctctgtggac ctatacccgc ctggcgatgc
     1381 gcgcgaatac ttttcaaagg agaacatcat gagcaagagt caggacgcaa agaagaacct
     1441 caagaaggcg ccgaccagga caccgaagga aaagaaagaa gccaagcggc tcaaaaaagc
     1501 agagaagaag cattgatcgc ttgacctgtc ttgccgggcg ctggctcgag ttgccgtgcc
     1561 cggtcgtgct accttccaat gccgcgcgct tgaacccgcc ggatcaatac cggtctcacc
     1621 ggtgcgcgcg ccaccaacgc gcttcccatg gcgcacgata ccgtacgagt ctagtgccca
     1681 tgttgtgttg ccatgattcc cttttccata cttgatcttt ctcccattcc caaaggcgcc
     1741 aatgccgcgg tcgctttgcg caatacgctg gaactcgcgc agcatgcgga aaagctggga
     1801 tacaggcgtt actggcttgc ggagcatcac aacatgccag gcatcgccag cgcagcgacc
     1861 tcgatcgtaa tcgcccacgt cgctggcgga acccgcacta tccgcgtggg ttcgggtgga
     1921 atcatgttgc caaaccattc gccattggtt gtcgcggaac aattcggcac gctggcgtcg
     1981 ctgtatccgg gtcgcatcga tcttggcctc ggccgcgcgc caggcaccga catgctgacc
     2041 gcgcgcgccc tgcgtcgtga catggaggac agcgtcgagc agtttccaca ggatgtgcag
     2101 gaactgcagc gttattttgc tgactccacg gagcggcaat ctatccgcgc tgttccgggt
     2161 gccggactta acgtccccat ctggctgctg ggttcaagtt tgttcagcgc gcaacttgca
     2221 gccatgctgg gattgccgtt cgcatttgcc tcgcatttcg cgcccgattt gatgcgccag
     2281 gcagtcgaaa tctatcgcaa ccggttccag ccctccgacc agcttgaccg gccttacgtc
     2341 atgctggcgg cgaacacgct tgccgcggac acggatgcgc aggcgcgcta ccagtttact
     2401 tcgcagcaac agtcgttcat caatctcagg cgcggcgcgc cgggccaggt gcctccgcca
     2461 atagatgaca tcgatgcgta ctggtctcct gcggaaaagg ccatggtgga acgttcgctc
     2521 gcaatatcgt tcgtcggctc catcgctagc gtcgaacgcg ggctccgcac cttcactgac
     2581 caattgaagc cagacgagct catgattacc gcgcacattt ttgatcaggc cgcgcggtta
     2641 cgctcactcg aactcgttgc aggagtgcgc gaacgattga atattacgca gactgaatcc
     2701 tgaatctccg tggcaatcag aaactttctt tgtaaggccg caggtcgatt tcctgtgtcc
     2761 atgcagagcg cgcctggcaa tgtagctgcc agtaggtttc cgcaatggcg tccacgctga
     2821 gcaggccgtc ctggccacgc tgctggatga cttccggccg gttgcgccgc aggcgctcgc
     2881 cgtcaatgcc gccgtcgatc acgacatgcg cgacgtggat gccctgtggg ccaaattcgc
     2941 gcgccatgct ttgcgccagc atgcgcagtc ctgctttggc tgcggcgaac tgggcgaaac
     3001 ctggtttgcc gcgcaagctg ccggaggcgc cggtgaacag caccgtgccg ctgccgagcg
     3061 gcacaaggcg ccgggcggct tcccggccga ccaggaatcc gccgaagcag ccgatgcgcc
     3121 agaattcctc gaacgtctcc gccggcagtt cacgaaagtc ctgcttgcga ttgttgcctg
     3181 cgttgtaagc gacgagatcg agcgcatcca aatcggcgcg cggcgccata gcatgatcga
     3241 acagccggat gacgtcttgt tcacgagtgg cgtccataac gaatgcttcc gcgctgccgc
     3301 ctttcgacac gatcgcacgc gcgacttgct ctattttgtc ggagctgcgc ccagcgacga
     3361 ataggtgatg gccgccctca gcaaaacgac ggcatagtgc ggcccccaat ccgagttccg
     3421 caccgacgcc gactacgatt gccgctttcc tacccattgt ggagtcctat gatgtgctgc
     3481 agccgtattg cgcatccggt cagcgcgata gcttggagct gtcccggccg aaccatgcgc
     3541 ctgccgcgat tgtcctcgtg atgaggatgc cgtatacgac caaagcaaat gccatcgcgg
     3601 cgaataacca gtcaacggag ccggtccaac gcagcaccag ccaaccacca ccgatcgcga
     3661 tgaccagccg gacgaaactg ccaatcaagg gccacagcaa ccggcccgca ccttgcgaag
     3721 caaaataaag ggacagtcca agtccgaaga aaccgaaggc aggtccgaca atgtgcagat
     3781 aggccgatcc ggtttccagc atacgcgtct cgtggccaaa taattcaagc caggcatgcg
     3841 gccatagggc ggctgccagg ccgatcgcct cggtgagcgc gaatgcaatc gcacctccgg
     3901 tcagtgcaat acgcagtgca cgcgactgct ggcccgcgcc aatgttcgtg cccacgagtg
     3961 caaccaacgg agcacccaac ccgaatacca gtggaatcaa caaatactcc agacgggcag
     4021 ctgtgccgta gccggcgact gcatcaggcc cgccggctgc gcccaccagc gcggtggcca
     4081 gtgcaaaaat aagcgtggtt tgcaatgtgt tgatggagcc gattgcgccc acgcgcagga
     4141 tgtccatgaa caatactttg cgcaggcgca cgagaaccgg ctgtaccacc gagcggccgg
     4201 cccagatgta ccacgccatg ccggccgcag ttagcgccgt agtgagtagc actgcgatac
     4261 cgcccccggc gatgccgagc gcagggatcg gaccgagccc gaagatcaac aagggcgata
     4321 gcggaatcag cagtatgacg ccgatgcata tcgcgatcga tgggaccagc atattgcctg
     4381 tgccgcgcaa cgcactggcc agtgcattca acagccacac cagcactgcc cctgcgaata
     4441 ccacattcga ataatccagc gcggcctgca gcgacgcagc ttcgcctccc attgcgcgat
     4501 agagttgcgg gccgaaagcg atgaacagga cgctgaacag caagccgagc gcgacattaa
     4561 ccacaatggc atgcatcacc agcgcgtttg catcgtcgcg ccgcgctgac ccgagggcgc
     4621 gcgcgatggc ggacgagata ccgccaccga ccgcccccgc cgacagcatc tgcatcatca
     4681 tgaagccggg gaaaaccagc gccatgccgg caagtgcgtc aatgcccagg cgcgatacaa
     4741 accaggtttc gataaggcct gttgctgcct gcgcccccat gacgaggata ttgggccacg
     4801 ccatgcgcaa caaggtcggc acgatgcgcc cttccagcaa caggcgggtg cgtggattga
     4861 gcgctgcgcg ccggacatct acgtccagcg ctgcggatgc cgggacactc acaggcgccc
     4921 ccgggatttg cgaatacgat gccggtcgaa cggcgacgaa ggggcggcga ccgtgtcgcg
     4981 accccacgcg ctcgattcca gtttgcgccg ggttgcttcc cccgcggccg gtccaggcgc
     5041 gatgacatat tccgggcccg ccagcggacg tccggtcttg cgatcgacca gcactggatc
     5101 cgccggcgcg ccagtcttcg catgcacgag cagcacgctc gccccttcgg gcgcgaaata
     5161 cttgttgccc caggcttgca tggcaacaat gaccggctga aaatcgcggc cgcgctcggt
     5221 cagaacatat tcgtctcgcg gcggacgttc gctgtagcgg cgcttttcca gcaaacccgc
     5281 cttgaccagt gcgccgagcc gccgggtcag catattgggc gcgatgccga gactttcctg
     5341 gaactgatcg aagcgcgtta atccatgcaa agcatcgcga atgatcaaca tgctccacca
     5401 ttcacccacc cgctccagac tgcgggccac cggacactgc atcttgccga agcttttgtg
     5461 tttcatgatc gcatgctagc ccagtaactt gcattatgca agtaactagc ctccggcatt
     5521 gaaccggcaa gccttgggga tgcactaaac tgtttcctgc ggcccatgcg cgacaaggcg
     5581 gaaaaatgat taccaggcta acggatctgc tcggcatcga gttgccgatc attcaggcac
     5641 cgatggcggg tgcacaggcc agcgccatgg cagtggccgt gtcaaacgcg ggaggactcg
     5701 gttcgattgg ctgcgcaacg ctcggcagcg aggcgttgcg caaagaactg actgctctca
     5761 aggcgcagac tagccgtccc tttaacgtta acttcttttg ccatgttccg cccgtggaag
     5821 acgcggtgcg ggaatcggcg tggcgcgaca agcttgcgcc ctattaccaa aaatatggca
     5881 tcgctgcagg cagcatccct gccggtccgg gtcgtgctcc tttcaccgcg gaagcggccg
     5941 atttgctgga ggacatcagg ccggccgtcg tgagctttca tttcgggctg ccggccacgg
     6001 acttgctggc gcgcgtgaaa agcctgggcg caaaaatact cggctgtgca acgacgctca
     6061 aggaagcgct ttggctggag gcgcgtggca tcgatgcaat cattgctcag ggcgtggaag
     6121 cgggcggaca tcgcggcaat ttcctgtcgg acgacttgac cacgcaagtc ggcacgtttg
     6181 ccctggtgcc gcagatcgtg cgcaccgtca aattgccagt cattgcagcc ggcggcattg
     6241 cggatgcgca aggcgtcagg gccgtcatgg cgctcggtgc tgccgctgcg cagatgggaa
     6301 cggcgtatct gctttgtccg gaagccacca tcagcgcgat tcatcgtgcg gcgctgaaaa
     6361 gcgaagcagc acgccatacg gcgctgacga acatttttac gggccgcccg gcgcgcggca
     6421 tcatgaatta tgcaatgaag gaactcggcc cgattaatca cgctgcgccg gcgtttccac
     6481 tggcgacttc ttctatcgcc ccattgcgtg caaaagcaga aagcgcaggt tcaggagatt
     6541 tcacgccgct gtggtcaggc cagaatgcaa ccggctgcaa ggaagtgccg gctgcgcagc
     6601 tcacacggga actcgcggga ggactgtagc ctgacgcaat cgcgtcaggc tagcaattgc
     6661 tggtggcggt cattcaaacg cgttggtca
//
LOCUS       DATMZQ010000338         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_831894, whole genome shotgun sequence.
ACCESSION   DATMZQ010000338 DATMZQ010000000
VERSION     DATMZQ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831894"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VN496_10745"
     CDS             <1..508
                     /locus_tag="VN496_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17474.1"
                     /translation="VPERHNAVKVLLFLDVGGSMEGYVKVCEELFSAARVEFKHLEYF
                     YFHNFLYESVWRDNRRRNAERTPTFQILHKYAHDYKLVLVGDASMSPYEIGYPGGSVE
                     HHNEEAGAVWLQRMLNVYHHAVWLNPTPEDRWSYYESIGMVSKLMGNRMYPLTLQGLE
                     TAMRTLTR"
     gene            complement(531..956)
                     /locus_tag="VN496_10750"
     CDS             complement(531..956)
                     /locus_tag="VN496_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXF17475.1"
                     /translation="MKNTLKAGLTHTFKFTIPPNKTVPFLYPESDLFRAMPDVLATGF
                     MVGLLEWACVEAIRPYLDWPAEQTLGTLVNFSHEAATPPGMTVTVRVKLSEINGRKLR
                     FDIEAHDGIDLISRGSHERVVVDAARFREKMAQKATRIP"
     gene            complement(1064..>2692)
                     /locus_tag="VN496_10755"
     CDS             complement(1064..>2692)
                     /locus_tag="VN496_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXF17476.1"
                     /translation="KWQDPRIPKKLLTLEVLTEWLAARVSLPYFHIDPFKIDFATVTK
                     VMSNAYAERYKILPVRVTGTEAVIATCEPFVREWEAELARILRLEVKRVIANPQDINN
                     FLVEFYNLARSVKSATELHGAALSDITNFEQLVQLGSSGKLDANDQHIVRLVDWLLQY
                     AFDEGASDIHVEPRRDVANVRFRIDGMLHQVYQIPTPVVSAMTSRIKILGRMDVVEKR
                     RPQDGRIKTRTSGGEEIELRLSTMPTAFGEKLVMRIFNPDVMVKGFKDLGFSDEDRDR
                     WQKAVTNPHGIVLVTGPTGSGKTTTLYSTLKQLSTSDVNVCTVEDPIEMVEPVFNQMQ
                     VQQGINLDFASGIRTLMRQDPDIIMVGEIRDLETADMAIQAALTGHLVLSTLHTNDAP
                     SAVTRLLDLGVPPYLIQSTILGVMAQRLVRTLCPHCREAGPIETDTWNMLIAPWQAEK
                     PQQVYYHKGCLDCRMTGYSGRVGIYEILLMTAGQRPLITGDTDASLIREQAYRDSMKP
                     LRISGALKVAAGITTVEEVLKVAPPLSGDRRKKT"
BASE COUNT          561 a          747 c          794 g          590 t
ORIGIN      
        1 ggtgcccgag cggcacaacg cggtgaaggt gctgctgttt ctggacgtcg gcggatcgat
       61 ggaaggctac gtcaaggttt gcgaggaact gttttcggcg gcccgcgtgg aattcaagca
      121 cctggaatac ttctactttc acaatttcct gtatgaatcg gtgtggcgcg acaaccgccg
      181 ccgcaatgcc gaacgcacgc ccacgtttca gatactgcac aagtacgcgc acgactacaa
      241 actggtcttg gtcggggatg cttcgatgag tccctatgag atcggctatc cgggcggaag
      301 cgtggagcat cataacgagg aagcgggcgc agtgtggctg cagcgcatgc tgaatgttta
      361 ccatcatgca gtctggctca atccgacgcc ggaggatcgc tggagctatt atgaatcgat
      421 cggcatggtc agcaaactga tgggcaatcg gatgtatccc cttacgctgc aggggctgga
      481 aaccgcaatg cggacgctga cccgctgagg caagcgcgtg aaacgcagca ctatgggatg
      541 cgagttgcct tttgcgccat tttctcgcgg aaccgcgctg cgtcgacgac aactctttcg
      601 tgagatccgc gcgaaataag atcgattccg tcgtgtgctt ctatgtcaaa gcgaagtttg
      661 cggccattga tttcgctgag cttaacgcgg acggtcacag tcatcccggg cggcgtagcc
      721 gcctcatgtg aaaaattaac cagggttcca agcgtctgtt cggcgggcca atcgaggtat
      781 ggacggatgg cttcgacgca ggcccattcg agcagcccga ccatgaatcc agtcgccagt
      841 acgtcgggca tggcgcgaaa caggtccgat tccggataca gaaaaggcac cgtcttattg
      901 ggcgggatgg tgaacttgaa ggtgtgggtc aggcccgcct tgagcgtatt tttcatgtta
      961 tgggtccgca ggaaagaggc acatttgcct gaaagctgac tggcaggaat accatcctgc
     1021 gccactacgc gataataaat agtccagtta gctgatacag cgatcaggtt ttcttcctgc
     1081 gatctccgct tagcggaggt gcgaccttca gcacctcttc caccgtcgta atgccggcag
     1141 caactttaag ggcgccggaa attctcaacg gtttcatgct atcccggtac gcttgctctc
     1201 gaatgagaga cgcgtcggtg tcgccagtaa tgagaggacg ttgaccagca gtcatgagca
     1261 ggatttcata gatgccgact cgcccggaat aaccggtcat gcggcagtcc agacaccctt
     1321 tgtggtaata cacctgttgc ggcttttccg cctgccacgg tgcaatcagc atgttccagg
     1381 tgtcggtctc gattggaccc gcttcgcggc aatgcgggca cagcgtgcgc accaggcgct
     1441 gtgccattac gcccaggatg gtcgactgga tcagatacgg cggcacgcca agatcgagca
     1501 ggcgcgtcac ggccgagggc gcatcgttgg tatgcagcgt agacagcacc aggtgaccgg
     1561 tcagcgctgc ttgaatcgcc atgtcggcag tctccagatc gcgtatttcc ccgaccatga
     1621 tgatgtccgg atcctggcgc atcaacgtgc ggatgccaga agcgaaatcg agattgatcc
     1681 cctgctgcac ttgcatctga ttgaagaccg gttccaccat ttcaatcgga tcttcgaccg
     1741 tgcagacgtt cacgtcgctg gtagagagct gcttcagcgt ggaataaagc gtcgtcgtct
     1801 tgcccgaccc ggtcgggccg gtcaccagca cgatgccgtg cggattagtg accgcttttt
     1861 gccagcggtc gcgatcctca tccgagaatc caagatcctt gaatcccttg accatgacat
     1921 cgggattgaa aatccgcatc accagctttt cgccaaaggc ggtcggcatg gtggaaaggc
     1981 gcagctcgat ttcctcgcct ccgctggttc gcgttttgat gcggccgtcc tgaggacgcc
     2041 gtttttcgac gacatccatg cggccaagga ttttgatgcg gctggtcatg gccgagacca
     2101 ccggcgtcgg aatctggtag acctggtgca gcatgccatc gatgcggaac cgtacatttg
     2161 cgacgtctcg ccgcggttca acgtgaatat cgctggcgcc ttcatcgaac gcatattgca
     2221 gcaaccagtc aaccaggcga acgatatgct ggtcgttggc gtctagcttg ccgctgctgc
     2281 ccagctggac cagctgttcg aagttggtga tatcggataa tgctgcgcca tgcagttccg
     2341 tcgcgctctt cactgagcgc gccagattgt agaactcgac caggaagttg ttgatgtcct
     2401 gcggattcgc gatgacgcgc ttgacctcga ggcgcaggat gcgcgcaagt tccgcctccc
     2461 actcgcgaac gaaaggttcg cacgtggcga tgaccgcctc cgtgccggtc acccgcacgg
     2521 ggagaatctt gtagcgctcc gcgtacgcgt tagacatcac cttggtaacg gtggcaaagt
     2581 cgatcttgaa cggatcgata tggaaataag gcagtgaaac gcgcgccgcc agccattcgg
     2641 taagcacctc gagggtcaat aattttttcg ggatgcgcgg gtcttgccat tt
//
LOCUS       DATMZQ010000339         9509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_624997, whole genome shotgun sequence.
ACCESSION   DATMZQ010000339 DATMZQ010000000
VERSION     DATMZQ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624997"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..685)
                     /locus_tag="VN496_10760"
     CDS             complement(32..685)
                     /locus_tag="VN496_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HXF17477.1"
                     /translation="MNLEQARINMVESQIRTWEVLDQTVLDLLLTIRREEFVPDRYRE
                     LAFADLELPLGYGEVMLAPKMEARMAQELDIRKTDKILEIGTGSGYVTALLAKLGGQI
                     VSVERIPELSQSATRKLAGHALQNVQLHVGDGCEGWPSQAPYDAMLLTGSVPLLSESF
                     RQQLAIGGRLLAVIGESPVMTAHLITRVSAHAFNSIGLFETSIPPLKNIKQPERFVF"
     regulatory      751..849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            891..2762
                     /gene="thiC"
                     /locus_tag="VN496_10765"
     CDS             891..2762
                     /gene="thiC"
                     /locus_tag="VN496_10765"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXF17478.1"
                     /translation="MNANPKFLSATAHVDEAAVKPLPRSRKVYLPGSRTDIQVPMREI
                     TQGDTPASFGGEANPALMVYDTSGPYTDPRATIDIRNGLQPMRERWIESRSDTEIMAG
                     PTSIYGRSRLDDPKLAQMRFNLHRTPRRARFGMNVSQMHYARKGIVTPEMEFIAIREN
                     QRRDALPEIITRQHAGEAFGAAMPRLITAEFVRDEVARGRAIIPANINHPESEPMIIG
                     RNFLVKINANIGNSALGSSIQEEVEKMTWAIRWGGDTVMDLSTGKHIHETREWIVRNS
                     PVPIGTVPIYQALEKVDGRAEELNWEIFKDTLIEQAEQGVDYFTIHAGVRLAYIPLTA
                     GRMTGIVSRGGSILAKWCLAHHKENFLYTHFEDICEIMKSYDVSFSLGDGLRPGSIAD
                     ANDEAQLSELRTLGELTNVAWRHDVQVMIEGPGHVPMHLIKENMDLQLKLCHEAPFYT
                     LGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKDDVKDGIIAYKI
                     AAHAADLAKGHPGAQIRDNALSKARFEFRWDDQFNIGLDPDKAKQFHDETLPQEGAKL
                     AHFCSMCGPHFCSMKITQDVREYAATLGVTDAEALEKGMADKAAEFVAAGAELYRKA"
     gene            2824..3648
                     /locus_tag="VN496_10770"
     CDS             2824..3648
                     /locus_tag="VN496_10770"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXF17479.1"
                     /translation="MDLPLMLKALILGVVEGITEFLPVSSTGHLILVGDLLDFNTEKG
                     KLFEIVIQCGAILAIVWEYRSRLASVVSRLGRDANAQRFAMNLLVAFLPVAILGLLFG
                     KAIKEVLFRPVPVAVAFIVGGLIILWAERRKHEIRVFSVDAMTSLDALKVGLAQTLAL
                     IPGTSRAGATIIGGLFFGLSRQAATEFSFFLAIPVLFAASGYELFKHRSTLSTADLGI
                     LSIGMVAAFVSAFFCVRWLLRYISRHDFTVFAWYRIAFGIVILATAYTGLVNWTVD"
     gene            complement(3678..4130)
                     /locus_tag="VN496_10775"
     CDS             complement(3678..4130)
                     /locus_tag="VN496_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXF17480.1"
                     /translation="MDDISKILNKAHERGQQLGLPYAGALLPAEAHAVMKAVPGAHLV
                     DVRTRAEWDFVGRVPGSLLIEWQTYPNSQPNPSFLEELRARAGESDVLMFLCRSGARS
                     HSAASAATNIGYSKSYNILQGFEGDKDPNGHRASVGGWQFAGLPWVQS"
     gene            4426..5835
                     /gene="glnA"
                     /locus_tag="VN496_10780"
     CDS             4426..5835
                     /gene="glnA"
                     /locus_tag="VN496_10780"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXF17481.1"
                     /translation="MAVADVFKTIKESDVKFVDLRFTDTRGKEQHVSVPAHAFGQDKF
                     DDGHAFDGSSIAGWKGIQASDMLLMPDPDSARLDPFSDEVTLNITCDVIEPSDGKGYD
                     RDPRSIAKRAEAYLKSSGIGDVAYFGPEPEFFIFDGVTWNIDMSGCFVKISSEEAPWS
                     TGRDFEGGNMGHRPPVKGGYFPVPPVDSLQDIRNAICLALEQQGVEVEVHHHEVAAPG
                     QCEIGTKFNSLVKRADWLQILKYTVWNVSHSYGKTATFMPKPIVGDNGSGMHVHQSVW
                     KGGQNLFAGNGYAGLSDFALYYIGGVIKHAKALNAITNPGTNSYKRLVPGFEAPINLA
                     YSAKNRSASCRIPHVASPKGRRVEVRFPDPTANPYLAFAALLMAGLDGVQNKIHPGDP
                     IDKNLYDLPPEEAAKVPHPCHSLDQALESLEADHAFLTKGGVFSEDMISAYIELKMEE
                     VTRFRMTTHPVEFDMYYSS"
     gene            6041..6490
                     /locus_tag="VN496_10785"
     CDS             6041..6490
                     /locus_tag="VN496_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXF17482.1"
                     /translation="MLLHPAAWAETCKYVDADGRVIYSNTPTSPPKGATKVKCFEDPK
                     AVAPTARNPETKPASPEGRAKAPRVSEDTQKSRDDDRRRILEQELADETKQLAQAKEQ
                     LVAQEAIRNGNERNYERFLERVQPYRDAVATHERNLEALKREIANLK"
     gene            6594..7649
                     /gene="glnL"
                     /locus_tag="VN496_10790"
     CDS             6594..7649
                     /gene="glnL"
                     /locus_tag="VN496_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HXF17483.1"
                     /translation="MAPLTFTGLDALATAVVFLDLQHVVRYVNPAAENLLAISAKNLV
                     GVPLDKVIADPELLIAAVNYAKENNCSYTQHELPLITPAHIRIEISCTVTPTEMGEFT
                     GFLMELSPLHQQLRIAREERLMDQTTQTRELIRNLAHEIKNPLGALRGAAQLLERELE
                     RPELHEYTQVIMKEADRLQSLMDRLLTPHRLPHVTHFSVHEALERVRSLLVAEFPDGI
                     TVHQDYDVSLPGLMADREQIIQALFNIARNGAQAMQGKGTLILRTRIARRVTLAKKMH
                     RLAILISIVDDGPGIPAELMDKIFFPLVSGREGGHGIGLTIAQTFINQHHGTIEVESR
                     PGETCFTILLPVDHAHG"
     gene            7707..9110
                     /gene="ntrC"
                     /locus_tag="VN496_10795"
     CDS             7707..9110
                     /gene="ntrC"
                     /locus_tag="VN496_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HXF17484.1"
                     /translation="MKPVWIIDDDRSIRWVFEKALSREDVLFKTFASAQEAMQALDSS
                     TPQVVVSDIRMPGSSGLELLQKMKEKHPALPVIIMTAYSDLESAVAAFQGGAFEYLPK
                     PFDVDHAVDLIRRAIEESLRQDDVAEVPQDSPEILGQGASMQEVFRAIGRLTQSHATV
                     LINGESGTGKELVARALHRHSPRASKPFIAINTAAIPKDLLESELFGHERGAFTGAQA
                     MRRGRFEQAEGGTLFLDEIGDMPPDLQTRLLRVLSDGQFYRVGGHQPIKANVRIIAAT
                     HQDLESRVKSGLFREDLFHRLNVIRLRLPALRERRDDIPLLARHFLQKSARELGVEPK
                     KMSEAAMKYLMAMDLPGNVRQLENLCHWLTVMAPGINVDVNDLPPELRDEVPQASAEN
                     WIAALEREADASLKRGESAILDELTRQFEKALITRALAHTGGRRIEASLLLGMGRNTL
                     TRKIQELGLTEEASRDK"
     gene            complement(9118..>9509)
                     /locus_tag="VN496_10800"
     CDS             complement(9118..>9509)
                     /locus_tag="VN496_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXF17485.1"
                     /translation="SFSKWLREESHKYSRIAVLGDFNIAPEDRDVHDPLAWQGQVLCS
                     DAERTAFSELIAVGFKDSFRLFEQPERLFSWWDYRMNAFRRKMGLRIDHILLSDGLSK
                     ECKSSGIDIAMRTNERPSDHAPVVATF"
BASE COUNT         2216 a         2584 c         2642 g         2067 t
ORIGIN      
        1 aaccttagcc gcacgcttgc gacgatcgcc tctagaaaac aaagcgctct ggctgcttga
       61 tatttttcag cggcgggatt gatgtttcga acaggccaat cgaattgaaa gcgtgcgccg
      121 agacgcgtgt aattagatga gcagtcataa ccggcgattc accaattacc gcaagcagcc
      181 ggcctccgat cgctagttgc tgccggaacg attcggatag cagcggaaca gaaccagtca
      241 ggagcattgc atcgtagggc gcttgtgagg gccaaccttc acagccgtcc ccgacgtgca
      301 attgaacgtt ctgcagtgca tggcctgcga gctttcgagt ggcggactga ctcaactcag
      361 ggatgcgctc cacggagaca atctgacctc ccagctttgc cagcagcgcg gtcacatacc
      421 cgctgcccgt tccgatttcg aggatcttat cggtctttcg tatatccagc tcctgcgcca
      481 tgcgtgcttc catcttgggc gccagcatca cttcgccgta acccagcggc agttcaaggt
      541 cagcaaaagc gagttcacgg tatctgtccg gaacaaattc ctcgcgcctg attgtcagca
      601 agagatccag aacggtctgg tcgaggactt cccaagtgcg tatttggctc tcaaccatgt
      661 tgatgcgggc ctgttccaga ttcattcgtg tgtgagctgg atggaggaat tttgaagctt
      721 gcaattatca cacaattcaa ttagctttcc tgactgcgtt gggggtcctg cgcacgaact
      781 gcgcgggtga gacaaaccct ttggacctgt acggataatg ccgatgcagg gaagcgcgtt
      841 gaagttttgg ccgcgctgtc ctgtaactcc ccagctatca ggagcgcatc atgaatgcca
      901 atcccaaatt tttaagtgcg actgcccatg tcgatgaggc cgcggtaaaa ccgctgccac
      961 gttcacgcaa ggtttaccta ccaggttcac ggaccgacat ccaggtgccc atgcgggaaa
     1021 ttacgcaggg cgatacaccc gcgagcttcg gtggagaagc caatcccgcg ctaatggttt
     1081 acgacacgtc gggtccgtat accgatccgc gcgcaacaat cgatattcgc aacggcctgc
     1141 agccgatgcg cgagcgctgg attgagtcac gttccgacac cgaaataatg gcaggaccaa
     1201 cgtcaattta cgggcggtcg cgtctggatg atccgaaact ggcgcagatg cgcttcaatc
     1261 ttcatcgcac gccccgtcgc gcccgcttcg gcatgaacgt cagccagatg cactatgcac
     1321 gcaagggcat cgtcacaccg gaaatggaat tcattgcgat acgcgagaat cagcgtcgcg
     1381 acgcattgcc tgaaatcatc acgcgacagc atgccggtga agcattcggt gccgccatgc
     1441 caaggcttat cactgcggaa ttcgtgcgcg acgaagtagc ccggggccgg gccattattc
     1501 cagcaaacat aaatcatccg gaatccgagc caatgatcat tgggcggaat tttctggtga
     1561 agatcaatgc aaacattggt aactcggcgc tgggttcgag cattcaggaa gaagtcgaaa
     1621 aaatgacgtg ggcgatacgc tggggcggcg acacggtcat ggatctttct accggaaagc
     1681 acattcatga aacacgcgaa tggatcgtgc gcaattctcc tgtcccgata ggcaccgtac
     1741 cgatttatca ggcgctcgaa aaggtcgatg gacgcgccga agaactcaat tgggaaatat
     1801 tcaaggacac gctgatagaa caggcggaac agggcgtgga ttacttcacc attcacgccg
     1861 gcgtgagact ggcatatatc ccgctcacgg caggacgcat gaccggaatt gtgtcgcgcg
     1921 gcgggtccat tcttgccaag tggtgcctcg cgcaccataa agagaatttc ctgtatacgc
     1981 atttcgagga catctgcgag attatgaaat cgtatgacgt gtcgttctcc cttggagatg
     2041 gattgaggcc gggttcaatt gcggatgcca atgatgaagc gcaactgagt gagttgcgca
     2101 cgcttggcga actaaccaat gttgcctgga gacatgacgt tcaggtaatg atcgaagggc
     2161 ccgggcacgt gccgatgcat ctcatcaaag agaacatgga cctgcaactc aagctgtgcc
     2221 acgaggcgcc cttttacacg ttggggcccc ttacgaccga cattgcgccg ggatacgacc
     2281 acattacttc agcgattggc gcagcgatga tcggctggta tgggacggca atgctgtgct
     2341 atgtcacgcc aaaggagcat ctgggtttac cggataagga tgatgtcaag gacggaatca
     2401 ttgcctacaa gatcgccgct catgccgcag acctggcaaa gggccatccc ggcgcccaga
     2461 tacgtgataa cgcgctgtcg aaagcgcgat tcgagtttcg ctgggacgac cagttcaata
     2521 tcggactgga tccggacaag gcgaagcaat tccacgatga aaccttgccc caggaaggtg
     2581 ccaagctggc ccatttctgt tccatgtgcg ggccacattt ttgttccatg aaaatcaccc
     2641 aagatgtgcg cgaatacgct gccacactag gggtaactga cgccgaggcg ctagaaaaag
     2701 gaatggccga caaggcagcg gaattcgttg cagccggcgc agaactgtac cggaaggcat
     2761 gagcaaccgg gttcggcccg acggtggcgg tcgtcactgg gttgcaatat ctcgtgaggt
     2821 tcaatggacc tgccgttgat gctcaaggcg ctcattcttg gcgtcgtgga aggaatcacc
     2881 gaatttttgc ccgtatccag cacgggtcat ttaatcctgg taggtgacct tctcgacttc
     2941 aacacggaga aaggcaagct tttcgagatc gtgattcagt gtggcgccat tctggccatc
     3001 gtttgggaat atcggtccag acttgcctct gtcgtctcaa gattaggccg tgacgccaac
     3061 gcacagcgct ttgcgatgaa cctgctggtt gcgtttcttc cggtcgctat cctaggcctg
     3121 ctattcggca aggccatcaa ggaagtactg ttcaggccag ttccggtagc cgtggcattt
     3181 attgttggcg gcttgattat cctgtgggcg gaacgccgca agcatgagat ccgggtcttc
     3241 agtgtggatg caatgacttc attggacgcg cttaaggtag gccttgcgca aacgttggcg
     3301 ctcatacccg gcacgtcccg ggctggggcg accattatcg ggggattgtt tttcggcctc
     3361 tcaaggcagg ccgccaccga attttccttc tttcttgcca tcccggtttt gttcgccgcc
     3421 tccggctacg agttgttcaa gcatcgcagc acgctttcta cagcggatct cggaatactg
     3481 tcgattggaa tggtcgctgc gttcgtatca gcgttctttt gcgtgcgctg gctactgcgc
     3541 tatatcagcc gacatgattt caccgtattc gcctggtatc gcatcgcatt cggtatcgtg
     3601 atcctggcca ctgcctacac tgggctcgtg aactggacgg tggattgaga ccaagaactt
     3661 tgtatttgcg tgttcgatca gctctgtacc cacggcaatc cggcgaactg ccagcccccc
     3721 actgaggcgc gatgaccgtt cgggtcttta tcgccttcaa aaccctggag tatgttgtaa
     3781 ctttttgaat atccaatgtt ggtggctgca gaagctgcag aatgagagcg tgcgccgctt
     3841 cgacaaagga acatgagcac gtcactttcc ccggcccgcg ctctgagttc ctccagaaac
     3901 gatgggttcg gctgagaatt cggataagtt tgccattcga tcaacagact tccaggcacc
     3961 ctgccgacaa agtcccactc ggcacgagtc cgtacatcga ccaagtgtgc gccggggacg
     4021 gctttcataa ccgcgtgggc ttcggcgggc agtaaggcgc cagcgtaggg cagccccaat
     4081 tgttgccctc tctcgtgagc tttattcaat atttttgata tatcgtccat accactcggg
     4141 cctcttaatt aacaatgcac aggtctgctg cataatccgc tgctcgtgtc gagagcgggc
     4201 aaattatgga cctggccaaa tttatcttgc caagacacat gccttgcact aatttcgggc
     4261 acccacgggc gttaggcttt tatttggtgc acaacaacgc taccaaaaca gtctaactct
     4321 ttgtgtgcca gtggtttatc ggatattttc gctgggcatg gttcatgctg acactgtggc
     4381 tcgccgaaaa ttcctcgcgc ttacttccca actcaggaga gctatatggc ggttgccgac
     4441 gtatttaaaa caattaaaga aagcgacgtg aagttcgttg acctgcggtt taccgacacc
     4501 cggggcaagg aacagcacgt ttccgtcccc gcgcacgcct ttggtcagga caaattcgat
     4561 gatggtcatg ccttcgatgg ctcttctatc gcgggctgga aaggcattca agcctccgac
     4621 atgctgctga tgcccgatcc cgattcggcg cgtctggacc ccttttccga cgaggtcacg
     4681 ctgaacatca cttgcgatgt gatcgaacca tcagacggca agggctacga ccgcgatccg
     4741 cgttcaatcg ccaagcgggc tgaggcttat cttaaatcca gcggcatagg agacgtcgcc
     4801 tattttggtc ccgaaccgga gttcttcata ttcgacggcg taacctggaa catcgacatg
     4861 tcaggttgct ttgtgaaaat ttcttccgaa gaagcaccct ggtccaccgg cagggatttt
     4921 gaaggcggaa atatgggcca tcgccctccc gtcaaaggcg ggtattttcc ggtcccccca
     4981 gtagattcgt tgcaggatat ccgtaatgcg atctgccttg cactcgaaca acaaggcgta
     5041 gaggtggaag tacatcatca tgaagtggca gcacccggcc agtgcgaaat cggtaccaag
     5101 tttaattctc tggtaaagcg tgccgactgg ctacagatcc ttaaatacac tgtctggaat
     5161 gtctcgcact cttacggcaa aacggcgacc ttcatgccaa aaccgattgt cggcgacaac
     5221 ggatccggca tgcatgtcca tcagtcggtg tggaagggcg gacagaatct gtttgcgggc
     5281 aacggctatg caggcctgtc ggattttgcg ttgtattaca tcggcggcgt gatcaaacac
     5341 gcaaaagcgc tgaacgcaat caccaaccca ggaacgaatt cgtataagcg ccttgtaccc
     5401 gggtttgaag cacccataaa tttggcgtat tcggcgaaga accggtccgc gtcctgtcgc
     5461 attccacacg ttgccagccc gaaggggcgg cgtgtcgaag tgcgttttcc cgatccgaca
     5521 gcaaaccctt atctggcttt tgcggccttg ctcatggcag gacttgacgg cgtacagaac
     5581 aaaattcatc cgggggaccc gatcgacaag aacttgtatg acttgccgcc agaagaagcc
     5641 gccaaggtcc ctcatccatg tcattcgctg gatcaggcgc tggaaagcct ggaagccgat
     5701 cacgccttcc tgaccaaagg gggcgttttt tccgaagata tgatcagcgc ctacattgaa
     5761 ctcaagatgg aagaggtgac gcgcttcagg atgacgacgc atcccgtgga atttgatatg
     5821 tactacagtt catagacagc attaaggacg aaaaagggcg ggaattcccg ccctttttcg
     5881 tttgcagtgg caaatctcac cggggtgcgc gcattgataa tggaaaataa ctgccttaga
     5941 cttcgacacg gactcgtgaa gcttcgccgc gcgtccttct tcgtccagct ggcaggggtt
     6001 acagatgcag ttccgccgac taacagcatt cttcgcagtg atgttgctgc atccggccgc
     6061 ttgggcggag acgtgcaaat acgtggatgc cgatggccgg gtcatctatt cgaacacacc
     6121 aaccagtccg ccgaaaggcg cgaccaaggt gaaatgcttc gaagacccaa aagcagtggc
     6181 gcctaccgcg cgaaacccgg agaccaaacc tgcttccccg gaaggcaggg ccaaagctcc
     6241 acgcgtaagc gaagacacgc agaagtcgcg cgacgacgac cggcgtcgca tactggagca
     6301 ggaactcgcg gacgagacca agcaacttgc gcaagcgaag gaacaactgg tcgcgcaaga
     6361 agccatccgc aatggaaacg aacgcaacta cgagcggttt ctggagcgcg tgcagcctta
     6421 tcgggatgct gtggcgactc acgaacgcaa tctcgaggcg ctgaagcgcg aaatagccaa
     6481 tctgaaatag gctgcaggct tgttatcgcc cctgttttct acacgggcac aaaatccaaa
     6541 cagtgtggtg taaatcttgc ggagctagcg cttgcctggg gcattgcgac gttatggctc
     6601 ccttgacctt taccggactc gatgcgcttg cgacggcagt ggtgtttctg gatctccagc
     6661 atgtcgtgcg ctatgtgaat ccagcagccg aaaatttgct ggcgattagt gcgaaaaatt
     6721 tggtcggcgt gcctttggac aaagtgatag cggatcccga gttattgata gcggccgtca
     6781 actatgcgaa ggagaacaat tgcagctaca cgcagcacga attgcctctg ataacgccgg
     6841 cgcacattcg gatcgaaatc tcgtgcacag ttacgccgac cgagatgggt gagttcactg
     6901 gttttttaat ggaattgagc ccgctgcatc aacagctgcg cattgcacgt gaagagcggc
     6961 tgatggatca aaccacgcag acacgggaat tgatccgcaa tctcgcgcac gaaataaaaa
     7021 atccgcttgg ggcgctacgc ggagcggcac aactgctcga acgggaactc gagcggccag
     7081 aactgcacga atacacgcaa gtcatcatga aagaggcgga cagactccag tccctcatgg
     7141 atcgactgct gacgccgcat cgcttgccgc atgtcacgca ttttagcgtc catgaggccc
     7201 tggagcgcgt gcgtagtctg ctagtggcgg aattcccgga cggaataacc gttcaccagg
     7261 actacgacgt aagcctgcca gggttaatgg ctgatcggga acaaattatt caagcgttat
     7321 ttaacatcgc gcgcaatggc gcgcaggcga tgcaaggcaa gggcactttg atcttgcgga
     7381 cccgtatcgc cagacgcgtt actttggcga agaagatgca caggcttgcc atactaataa
     7441 gcatcgtcga cgacggaccc ggcatccctg ctgaactcat ggacaagatc ttttttccat
     7501 tggtctcggg tcgcgaaggc ggacacggta tcggattgac catcgcccag acgtttatca
     7561 atcagcatca cgggaccatt gaagtggaaa gccggccggg tgaaacatgc ttcaccatcc
     7621 tgttgccagt ggaccacgcc catggatgac attgcttccg ggtgcgacat ttgcgactcg
     7681 ccaccaacca aaattgacca tcgaatatga aacctgtctg gatcatcgac gacgatcgct
     7741 caatccgctg ggtgtttgaa aaagcactct cacgcgaaga tgtgctattc aagacgtttg
     7801 cttccgctca ggaagcaatg caggcgctgg actcctccac cccgcaagtc gtggtaagcg
     7861 atattcgaat gccgggcagt tcggggctgg aactgctgca gaaaatgaaa gaaaagcatc
     7921 cagctctgcc ggtcattatc atgacggcat attccgatct agagagtgcc gtcgcggcct
     7981 tccagggcgg ggccttcgag tatttgccca agcccttcga tgtggaccat gccgtggatt
     8041 tgatccggcg agccatagag gaaagtctgc ggcaggatga cgttgcggaa gtgccgcagg
     8101 attccccgga aatcctgggc cagggtgcct ccatgcaaga ggtgttccgc gccatcggcc
     8161 ggctcaccca atcgcatgcg acggtactga tcaatggtga atcgggaacg ggtaaggaat
     8221 tggtcgcgcg cgcgctgcac cggcatagtc cgcgagccag caagcctttc atcgctataa
     8281 acacggccgc gattccgaaa gatctgttgg aatctgaatt atttggccat gagcgcggcg
     8341 cttttactgg tgcacaggca atgcgtcgcg gacgttttga acaggctgaa ggcgggacac
     8401 tgttcctgga tgagataggc gacatgcccc ccgaccttca aacgcggctg ttacgggtgc
     8461 tttcggacgg gcaattttac cgagtgggtg gccatcagcc gatcaaggca aacgtacgaa
     8521 tcatcgcggc cacgcaccag gatttggaga gccgggtcaa aagcggatta ttccgtgagg
     8581 atctttttca ccgactgaat gtaatccggc tgcgattgcc tgctctgcgc gagcgccggg
     8641 acgacattcc gctgcttgcg cggcatttcc ttcagaagag tgcgcgcgag ctcggcgtgg
     8701 aacccaaaaa aatgtccgag gcggccatga aatatctgat ggcgatggac ctgccgggta
     8761 acgtgcgcca actcgagaat ctctgccatt ggctcacggt catggcaccc gggataaatg
     8821 tcgatgtcaa cgacctgccg ccggaattgc gagacgaggt tccgcaagcg tccgccgaaa
     8881 actggattgc ggcacttgag cgcgaagcgg atgcttcgtt gaaacgagga gagagcgcaa
     8941 tccttgatga actgacgcgt caattcgaaa aagctttgat tacgcgtgca ttggcacaca
     9001 ccggtggacg acggatcgag gcgtcgctac tgctcggcat ggggcgcaat acgctgaccc
     9061 ggaagatcca ggaactcggt ttgaccgagg aagcaagcag agacaaatga tgccggtcta
     9121 gaacgtggcc acgaccggcg catggtcaga cgggcgctcg tttgttcgca tcgcaatatc
     9181 tatcccactt gatttgcact ccttcgaaag tccatcggat agcagaatgt gatcaatgcg
     9241 cagacccatc ttgcggcgga atgcgttcat acgataatcc caccacgaaa ataaccgctc
     9301 gggctgctcg aataatcgaa agctatcttt gaaaccgaca gcgatgagct cactgaaggc
     9361 agtccgctca gcatcactgc acaatacctg gccctgccat gccagcggat cgtgtacatc
     9421 gcggtcctcc ggcgcgatat tgaaatcacc taacacggca atgcgggaat atttgtgcga
     9481 ctcttcccgc agccacttcg aaaacgagt
//
LOCUS       DATMZQ010000340         5107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2006410, whole genome shotgun sequence.
ACCESSION   DATMZQ010000340 DATMZQ010000000
VERSION     DATMZQ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006410"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VN496_10805"
     CDS             <1..595
                     /locus_tag="VN496_10805"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HXF17486.1"
                     /translation="GFRRSGAFTYRPYCDHCRACVPVRIDAKDFTANRSQRRAHARHA
                     ALEARMLELKYHPEHYSLYLRYQSHRHSGGGMDHDSREQYRHFLLQSNVRSSLVEFRE
                     DGKLRMVSIVDRLEDGLSSVYTFFEPDIAGASLGTFNVLWQIDLCRRLRLPFLYLGYW
                     ISQSRKMAYKNNFQPLEGLIDGQWRRLPRDAASPAAV"
     gene            642..1679
                     /locus_tag="VN496_10810"
     CDS             642..1679
                     /locus_tag="VN496_10810"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXF17487.1"
                     /translation="MNLYQLIRPLLFSLEPENAHRFTMRLLEFAHQTKSIRLVAGERP
                     IVSPRSVMGLSFPNPVGLAAGLDKNGEHIDALAALGFGFIEIGTVTPRPQPGNPRPRM
                     FRLIRDRAIINRMGFNNKGVDRVLNNISRTRYRGILGINIGKNFDTPLERAIDDYVYC
                     LRKVFTAASYVTVNISSPNTPNLRQLQDEGELDRLLEALVTERGRLTQLHGRRVPLAV
                     KIAPDLTQRDIATMASLFVKHGIDGVIATNTTVSREGVSQVRDADQPGGLSGAPLKTR
                     STKIVAQLAVVLAGRVPIIAAGGILHAADALEKMAAGASLVQVYSGLVYEGPALIRNI
                     TRALAASNSTQ"
     gene            1777..>5107
                     /locus_tag="VN496_10815"
     CDS             1777..>5107
                     /locus_tag="VN496_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF17488.1"
                     /translation="MNRIADLPTTPQLKLRFDVRLEDLYRRDGLLKVDAAFVDFLGRV
                     DPALSQQLELLRQQDPIALTRKQESQFLIDVAVHLDNFIAALFNIEDQVVALAQRHSE
                     LAPLYSCKRLFVQRKAANAYKAEVAAGFDGALLEAQLVTWFGTEFSELEFARQITAWQ
                     KDEAANAEKLAVALRYAAWAVHTAQGRQKHHAGVLFKVPKKLDFLRLVPVETRSENGV
                     AVHTLSHLRRRHGFALTDPGTDLIGALDETNYCIWCHEQEKDSCSHGLKEKPVAAGTT
                     PPFRNNVFGVPLNGCPLEERISEFQKLKSEGVSIGALAMITLDNPMAAATGHRICNDC
                     MKSCIYQKQEPVDIPQAETRTLKDVLELPWGFEIYSLLTRWNPLDLRRPVPRAPSGRR
                     VLIAGMGPAGFTVAHHLLNDGHTVVGVDGLKIEPLPMQLSGIDLTGARAAFEPIHDVA
                     TLYESLDDRVMAGFGGVAEYGITVRWDKNFLKVIRVLLQRREQFALFGGVRFGGTMEV
                     EDAFSLGFDHVVLAAGAGRPTVLDIPNGLAPGVRTASDFLMALQLTGAAKNNSIANMQ
                     LRLPVVVIGGGLTAIDTATESLAYYPVQVEKFLQRYELLCAEGNEAGVRGAWTLPERQ
                     VADEFLAHARAIRSERARAAAAGREAQILELLKSWGGVTLAYRKRLVDSPSYTLNHEE
                     VEKALEEGIRFAENVTPAAVQVDEFGYASGVAMHATGTNEDGSTRPFDVTLPARTVLV
                     AAGTQPNTVLAREDPRNFTLDGRYFQACDEDGNPVKPEKSAKPDAPRVLLSRRDDGRF
                     VSFLGDLHPSFFGNVVKAMGSAKLGYPVISRVLANVRPASTASDEVFVAQLNEQLRAT
                     VHEVIRLTPNIVEVVVHAPQAARKFKPGQFYRLQNFESLSRTVDDTRLAMEGLALTGA
                     WVDPKRGLVSTIVLEMGGSSDLCALLTPGEPVILMGPTGSATEIPSNQTVTLVGGGLG
                     NAVLFSIGQALRAAGSQVLYFAGYKKIIDRYKIKEIEAAADVVVWCCDEAPGFAPRRP
                     QDRNFVGNIVQAMTAYASGKLGEQRIAFSDTDRIIAIGSDRMMAAVAAARHGVLAAHL
                     KPHHFAIGSINSPMQCMMKEIC"
BASE COUNT         1041 a         1501 c         1532 g         1033 t
ORIGIN      
        1 cggttttcgc cgcagcggcg cctttactta tcgcccttac tgcgatcact gccgcgcctg
       61 cgtaccggtg cgcatagacg caaaggactt taccgccaac cggtcacaac gccgcgcaca
      121 tgcccgccat gctgccctgg aagcacgcat gctcgagctg aaataccacc cggaacacta
      181 ctcgctttac ttgcgctacc aatcgcatcg ccatagcggc ggcggcatgg accatgacag
      241 tcgcgagcag taccgccact ttctcctgca aagtaatgtg cgttcgagcc tggtcgagtt
      301 ccgtgaagac ggaaaattgc gcatggtgag catcgtcgac cggcttgaag acggtttatc
      361 ttcggtctac acctttttcg aaccggacat tgccggcgcc agtctcggca cctttaacgt
      421 actgtggcag atcgatttgt gtcggcggct gcggctgccc tttctttacc tcggctactg
      481 gatttcacag agccgcaaga tggcctacaa gaacaacttc cagccgctcg aagggttgat
      541 cgatggccag tggcggcgac tgccccggga cgcggcatcc cccgcggctg tctgaggtcg
      601 cgcgcggcgt ccgtcgcgaa cgcccggata cgggcgcagc tttgaacctc taccaactca
      661 tccggccatt actgttttca ctggaaccgg agaatgcgca ccggttcacg atgcgcctgc
      721 tggaatttgc gcaccagaca aagtcgattc ggctggtcgc tggtgagcgc ccgatcgtgt
      781 cgccgcgaag cgtgatgggc ctatcatttc ccaatcccgt cggactggct gcaggcctgg
      841 acaagaacgg tgagcacatc gacgcgctgg ccgcacttgg ctttggcttc atcgagatcg
      901 gcacggttac cccgcggccg cagcccggca atcccaggcc gcgcatgttt cgcctcatca
      961 gggatcgcgc cattatcaac cgcatgggct tcaataacaa aggggtcgac cgcgtgctca
     1021 acaacatctc ccggacgcgc tatcgcggaa ttctggggat taatatcggc aaaaacttcg
     1081 acacgccact ggaacgcgcg attgacgatt acgtgtattg cttgcgcaag gtctttacgg
     1141 cggcaagtta cgtcacggtc aacatttcgt ccccgaacac tccgaatttg cgccagttgc
     1201 aggacgaagg tgaactcgat cgcctgctcg aggcgcttgt cactgaacgc ggccggctga
     1261 cccagcttca cggcagacgt gttccgcttg ccgtaaaaat tgcgcccgac ctgacgcaac
     1321 gggatattgc gaccatggct tcgctattcg tcaaacatgg aatagacgga gtgatcgcca
     1381 caaatacgac ggtgtcgcgc gaaggcgttt cacaagtgcg ggacgcggac cagccgggcg
     1441 gcctcagcgg ggcaccgctc aaaacccggt ccacgaaaat cgtcgcgcaa ctggctgttg
     1501 ttctcgctgg ccgcgtgccg atcattgccg caggcggcat cctgcacgcg gccgatgcac
     1561 tcgagaaaat ggccgcaggc gcaagccttg tgcaggtgta cagcggactg gtctacgaag
     1621 gtcctgccct gatccggaac attacccgtg cgctggccgc cagcaacagc acgcagtgaa
     1681 cttcattatt accatagtat ccgcctgcat aagagcggtg ccgccgcgga gttataattt
     1741 tgggtttgac cgaggcgctc caatcaagct ggctgcatga accgtatcgc tgaccttccc
     1801 accactcccc agctgaagct gcgatttgat gtgcggctgg aggaccttta tcgccgagac
     1861 ggacttttga aagtggatgc ggcgttcgtc gattttctcg ggcgcgtgga tccggcgctg
     1921 agccaacagc ttgaattact ccgccagcag gacccgatcg cgcttacacg caagcaggaa
     1981 tcgcaattcc tgatcgacgt agcagttcat ctggataatt tcatcgcagc actgtttaac
     2041 attgaagatc aggtggtcgc gctggcccag cgccactctg aactcgcccc gctctattcg
     2101 tgcaagcggc tgttcgtgca acgcaaagcg gccaatgcgt acaaagcgga agtcgcggcc
     2161 ggtttcgacg gtgccctgct cgaagcccag ctcgtcacct ggttcggcac tgagttcagt
     2221 gaattggagt ttgcccgtca gatcactgcg tggcagaagg atgaagccgc gaacgctgaa
     2281 aaactcgctg tcgcgttgcg ctacgcggcg tgggccgtac acacagcgca aggacggcaa
     2341 aagcatcatg ccggcgtgct tttcaaggta ccgaagaaac tcgactttct gcgcctcgtg
     2401 ccagtagaaa cgcgcagtga aaacggggtg gcggtgcata cgctgtccca cctgcgccgt
     2461 cgccatggat tcgcgctgac ggatccgggc acggacctta ttggtgcgct ggacgaaacc
     2521 aactactgca tctggtgcca cgagcaggaa aaggattcgt gttcgcatgg cttgaaggaa
     2581 aagccagtgg ctgccggcac cacgccccct ttccggaaca acgtgttcgg cgtgccgctg
     2641 aatgggtgtc cgctggaaga gcgcatctcg gaattccaga aactcaaaag cgaaggcgtg
     2701 tccatcggcg ccctggcgat gatcacgctg gacaatccga tggccgcggc gacaggccac
     2761 cgcatctgca acgactgcat gaagtcctgt atctaccaaa agcaggagcc ggtcgacatc
     2821 ccgcaagcgg agacgcgcac gctgaaggat gtgctggagc tgccgtgggg ctttgaaatt
     2881 tactcactgt tgacgcgttg gaacccgctt gatcttagaa ggccggttcc gcgcgcgcca
     2941 agcggtcgac gtgtcctgat tgccggcatg ggccccgctg gcttcactgt ggctcatcat
     3001 cttctcaacg acgggcacac ggtcgttggc gtggatggct tgaagatcga accgctgccg
     3061 atgcaattgt cgggaataga cctcaccgga gcccgagcag cattcgagcc gatccatgat
     3121 gttgcaacat tgtatgaatc gctggatgac cgcgtcatgg cgggcttcgg tggggtcgct
     3181 gaatacggca ttacggtgcg ctgggacaag aattttctga aagtcatccg tgtgctgttg
     3241 cagcgccgtg agcagttcgc gttgtttggc ggcgttcgtt tcggtggaac gatggaagtg
     3301 gaagacgcct tttcgcttgg cttcgatcat gtcgtgctcg ctgcaggcgc gggacgaccg
     3361 actgtgctcg atattccgaa tggcctggcg cccggcgtac gcaccgcatc agactttctg
     3421 atggcgttgc agctcaccgg tgcggcgaag aacaattcca ttgccaacat gcagctgcgg
     3481 ttgccggtcg tcgtgattgg cggcggcctg accgccatcg atactgcaac cgaatcgctg
     3541 gcttattacc cagtgcaagt cgagaaattc ctgcaacgct acgaattgct atgcgcagaa
     3601 ggaaacgaag caggtgtgcg cggcgcctgg accttgccgg agcgccaggt cgcggacgaa
     3661 tttctggcgc acgcgcgcgc gatacgatca gaacgcgcga gggccgctgc ggcgggtcgt
     3721 gaagcgcaga ttctggaatt gttgaaatcg tggggcggcg taacgttggc gtaccgcaaa
     3781 cggctggtgg atagtccgtc ctatacgctc aaccacgagg aggtagagaa agccctggag
     3841 gaaggcatcc gcttcgctga gaatgttacg ccagcagcag tccaggtcga tgaattcggt
     3901 tacgccagcg gcgtcgccat gcatgcgacc ggcaccaacg aagacggcag tacaaggccg
     3961 ttcgacgtca cactgcccgc gcgtaccgtg ctcgtggctg cgggcacgca acccaatacg
     4021 gtgctggcgc gcgaagatcc gcgcaatttc actttggatg gccgatattt ccaggcttgt
     4081 gatgaggacg ggaatcccgt caagccggag aaaagcgcca aaccggacgc gccgcgggtg
     4141 ctgttatcca gacgcgacga cggccgcttt gtgagttttc ttggcgactt gcatccgtct
     4201 ttttttggga acgtagtgaa agccatgggg agtgccaagc taggctatcc ggtcatcagc
     4261 cgcgtactgg ccaatgtgcg gcccgcctcg accgcgtcag acgaagtatt cgtcgcacag
     4321 ctgaacgagc aactccgggc tacggtgcat gaggtgattc gcctcacacc gaacatcgtc
     4381 gaagtcgtgg ttcacgcgcc gcaagccgca cgcaaattca agcccggaca attctatcgg
     4441 ttgcagaatt tcgaaagcct ttcccgcacg gtcgacgaca cgcggctggc gatggaaggt
     4501 ctggcgctta ccggcgcatg ggttgatccg aagcggggcc tggtctccac gatcgtactg
     4561 gaaatgggcg gctcatctga cctgtgcgct ttgctgacgc cgggtgaacc ggtgattttg
     4621 atgggcccaa ccggttccgc caccgagatt ccctccaacc agaccgtgac cctggtcggt
     4681 ggtggcctgg gcaatgccgt gctgttttcg attggccagg cattgcgcgc cgccgggtcg
     4741 caagtgctgt atttcgccgg ttacaaaaaa atcatcgacc gctacaaaat caaagaaatc
     4801 gaggcggccg ccgacgtggt ggtctggtgc tgcgatgaag cgcctggctt tgcgccgcgg
     4861 cgcccgcaag atcgcaattt cgttggcaat atcgtgcagg ccatgacggc gtacgccagc
     4921 ggcaagctgg gcgaacaacg cattgcattc agtgacaccg atcgaatcat tgcgatcggt
     4981 tccgaccgca tgatggctgc agtggccgca gcgcgccacg gcgtgctggc cgctcatcta
     5041 aaaccgcatc attttgcgat tggttcgatc aattcgccga tgcaatgcat gatgaaggaa
     5101 atctgcg
//
LOCUS       DATMZQ010000341         2961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3042047, whole genome shotgun sequence.
ACCESSION   DATMZQ010000341 DATMZQ010000000
VERSION     DATMZQ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042047"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VN496_10820"
     CDS             complement(<1..427)
                     /locus_tag="VN496_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXF17489.1"
                     /translation="MSNFGLGISLFGAMLTLMVLRVPISVAMFVPGAVGYWLLAGDAA
                     LLNHLKGAIYARFSSYDLSVIPLFILMGQFATQGGLSRSLFKFASSLIGHLRGGVAMA
                     AIVACALFGAVCGSSVATAATLAQVALPEMRRLNYSGRLA"
     gene            complement(424..930)
                     /locus_tag="VN496_10825"
     CDS             complement(424..930)
                     /locus_tag="VN496_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXF17490.1"
                     /translation="MSMSATPAAGRPGLMEKLAAFSALVGGAILTGITVMAVVSITLR
                     SLGFQPIQGDFELVQVVLAGCIALMLPWCQLQGGNLTVDFFTVRLQRKKQRALDAVGS
                     LLFAIVMAVLTWRTWVGAIDVYASHESTMILGFPLWIGYAAMIPGLLLTTLSALHTAR
                     MAWKEAKS"
     gene            complement(1048..1932)
                     /locus_tag="VN496_10830"
     CDS             complement(1048..1932)
                     /locus_tag="VN496_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17491.1"
                     /translation="MGTLNLMVYRHSAFYSPLIAGIAGGFFAAEGFEPSYAVMAPGKT
                     VGEMLVSGTIHVAQTAVSGAWPYLEKSERPPYVNFAQINQRDGFLIASRNPVPEFGWA
                     KLTTGKFMFAHGGQPQAMLAYAMHKKGVSLDKTSGIDAGGPQKSMNAFRGGQGDWYHE
                     QAPYPQQLHHEGLADVVASVGEAIGPVAFSSLAALPRWLSSPDAIRFTRAYRKSRAWV
                     QSAPAATVALTLQSFFPDIAPDAMIAAIHDYQKLGCWEGTIEIDPAHYETALDVFSYS
                     RLITKRHAYDKVVVPPPA"
     gene            1989..2387
                     /locus_tag="VN496_10835"
     CDS             1989..2387
                     /locus_tag="VN496_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17492.1"
                     /translation="MSDSRRRGIFRIIRSRQDALNVVTDTSIVFLLVAAFLAGLYVME
                     GERILLHVGLFGFLGVMLWRFKSPIAAITLLLESIMWLFVTVALFVDTGELNWIYVTI
                     TAIVILTSIRAAEATLKLIGRYAAENRTED"
     gene            complement(2484..>2961)
                     /locus_tag="VN496_10840"
     CDS             complement(2484..>2961)
                     /locus_tag="VN496_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF17493.1"
                     /translation="GFASSPASLLGDAFGVLGGIFWAATTVWIRATGLTRISAAKVLF
                     YQLAVSAATLPAASWLMGEPGVIDVTPMAVASILYQGVIVAFATYLAWFWLLTRYLAG
                     RLAVFGFLTPLFGVVLGVLLLGEPLRPAFLGALAMVGMGIWLVNKRTERVKSRTEL"
BASE COUNT          632 a          864 c          896 g          569 t
ORIGIN      
        1 tggcaaggcg gcccgagtag ttgagccggc gcatctcggg cagcgcgact tgcgcaagcg
       61 ttgcggcggt ggcgaccgac gagccgcaca ccgcgccgaa caacgcgcag gccacgatcg
      121 cggccatcgc cacgccgccg cgcaaatgcc cgatcaacga gctggcgaac ttgaacagcg
      181 aacggctcaa tcccccctgg gtcgcgaact gccccatcag gataaatagc ggaatgaccg
      241 acagatcgta gctcgagaag cgcgcataga tggcgccctt gagatggttg agcagggctg
      301 catcgcccgc gagcagccag tatccgaccg cgccaggcac aaacatcgcg accgaaatcg
      361 gcacgcgcaa caccatgagc gtcagcatgg cgccgaacag cgaaatgccg agtccgaagt
      421 tgctcatgac tttgcttctt tccaggccat gcgcgcggtg tgcaaagccg ataatgtggt
      481 gagcagcagc cccgggatca ttgccgcgta gccaatccaa agcggaaagc cgaggatcat
      541 ggtgctttca tggctggcat agacatcgat cgcaccgacc caggtgcgcc atgtcagcac
      601 ggccatgaca attgcgaaca acagtgagcc gaccgcgtcc agcgcgcgct gttttttacg
      661 ttgcagccgt acggtgaaga aatcgacggt gagattgccg ccctgcaact ggcaccacgg
      721 cagcatcaag gcgatgcagc ctgccaggac gacctgcacc agctcgaagt caccctggat
      781 gggctggaag ccaagcgaac gcagggtgat cgataccact gccatcactg taatgccggt
      841 caggattgcg ccgcccacca gcgcggagaa cgcggcgagc ttttccatca ggcctggcct
      901 gccagcggcc ggtgtcgcgc tcatgctcat gacgggttga atcgggccgg cagccggttc
      961 gttgagtagg attgctttta ttatcgggcg gccaactgtt ggatgtccaa tattcctggc
     1021 cgctgcaccg gcgacggcga ttcgctgcta cgccggcggc ggtaccacta ctttgtcata
     1081 tgcgtggcgc tttgtaatga gcctggaata ggaaaatacg tccagcgcgg tttcgtagtg
     1141 cgccggatca atttctatcg tgccttccca gcagccgagc ttctgatagt cgtgtatcgc
     1201 agcgatcatt gcgtccggag cgatgtcggg gaagaacgat tgcagggtca gtgcgaccgt
     1261 cgcggccggc gcgctctgca cccatgcgcg tgacttgcga taggcacgcg taaaacggat
     1321 tgcgtcgggc gatgacagcc agcgcggcag agcggcaagg ctgctgaaag ccaccggtcc
     1381 gattgcttcc cccacggagg ccacgacgtc ggccagccct tcgtggtgca actgttgcgg
     1441 ataaggcgcc tgttcgtggt accagtcccc ctggccgccg cgaaaggcat tcattgattt
     1501 ttgtggaccg cccgcgtcga tgcctgaagt cttgtcgagc gacacgcctt tcttgtgcat
     1561 ggcataagcc agcatcgcct gcggctggcc gccgtgcgcg aacatgaatt tgccggtcgt
     1621 cagtttggcc cagccgaatt ccggcacggg attgcgcgaa gcgatcagga atccgtcgcg
     1681 ctggttgatc tgggcgaagt tgacgtacgg cggccgttcg cttttttcca gataaggcca
     1741 cgcgccggag actgccgttt gcgcgacatg gatcgttcct gaaacgagca tttctccgac
     1801 ggttttgccc ggagccatga cggcgtagct gggctcgaag ccctcagcag caaaaaaacc
     1861 gccggcgata ccggcgatga gcgggctgta gaacgcagag tggcgataca ccatcagatt
     1921 gagagttccc attgcgacca ttatgattcg ttccggcaat ttgtgccgaa gcattcggga
     1981 gaaacgactt gtcggacagc aggcgcaggg gcatcttcag aatcatccgc agccggcagg
     2041 atgcgttgaa tgtggtgacc gacacgtcga tcgtgttcct gctggttgct gcttttcttg
     2101 cgggccttta tgtcatggaa ggcgaacgca tcctgctcca cgtcgggttg ttcggatttc
     2161 tgggggtaat gctgtggcgc ttcaagagcc cgattgcagc cattacgctg ctgctcgaat
     2221 cgatcatgtg gctgttcgtg acggtggcgc tgtttgtcga tacgggcgaa ctgaactgga
     2281 tttacgtaac gatcaccgcg attgtgatct tgaccagcat tcgcgccgcc gaggcgacgc
     2341 tcaagcttat cggacgctat gcggccgaga ataggactga ggactgaggt atgaggactg
     2401 agtgaagtaa aaagcaggac cgagtaaagg cgaggacggt cttttgtgat tttatttttt
     2461 tgcttttact cagtcctcag tcctcatagc tcagtcctgc ttttcactcg ctcagtcctt
     2521 ttattaacta accatatccc catcccgacc attgcgagcg cgccaagaaa agccgggcgc
     2581 agcggttcgc cgagaagcaa cacgcccaag accacgccaa acaacggcgt gaggaatccg
     2641 aacacagcca gacgtcctgc cagataacgg gtcagcagcc agaaccaagc gagataggtt
     2701 gcgaaggcga caatgacgcc ctggtaaaga atgcttgcca cggccattgg cgtgacatcg
     2761 atcacgccgg gttcacccat cagccacgat gcagcgggca acgtggccgc ggacacggcg
     2821 agttgataaa acagcacctt tgccgcactg atgcgtgtca gaccagtagc acgaatccaa
     2881 acggtggtcg ctgcccagaa aatcccgccc aggacgccga atgcatcgcc cagcagcgac
     2941 gcaggcgatg acgcgaagcc a
//
LOCUS       DATMZQ010000342        15442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3386401, whole genome shotgun sequence.
ACCESSION   DATMZQ010000342 DATMZQ010000000
VERSION     DATMZQ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3386401"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VN496_10845"
     CDS             <1..505
                     /locus_tag="VN496_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HXF17494.1"
                     /translation="YQGIVSIEGDSIAQVLEHYMAKSEQLETRLWLASDSSQAAGMLL
                     QKLPATLSEDADAWNRATRLGETIKPEELLSLPAPEIIRRLYHQEDVRVFEPRPVAFR
                     CSCSHDRVTSMLRMLGHDEVRSIIEERGNVEVDCEFCGRHYVFDAVDAEQVFAADVIL
                     PVEKTRH"
     gene            646..1041
                     /locus_tag="VN496_10850"
     CDS             646..1041
                     /locus_tag="VN496_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXF17495.1"
                     /translation="MSESDTRVRIDKWLWAARFYKTRSLAADAVDSGKAKLNGERVKP
                     AKMLKTGDTLNIRNGLLSWEVTVLLLSDRRGSAMVASTLYSESDDTRRQREQQSAMLK
                     AERQANPFPGGRPTKRARRQIIRFIREQE"
     gene            1204..2379
                     /locus_tag="VN496_10855"
     CDS             1204..2379
                     /locus_tag="VN496_10855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXF17496.1"
                     /translation="MDDVVIVAALRTGVGKFGGTIGKIPATELGAKVITALLARTGVG
                     PELVSEVIMGQVLTAAAGQNPARQASIMAGVPNTVPAMTINKVCGSGLKATHLAAQAI
                     KAGDADIVIAGGQESMTLAPHALNGVRDGLRMMDGKLVDTMIIDGLWDVYNQYHMGVT
                     AENVAKKWNVSRQEQDEFAAASQQKAEAAQKAGRFRDEIVPVTISTRKGDVLFDTDEY
                     VRHGTTVEALASLKPAFSNSGTVTAGNASGLNDGAAAVMMMSTAKAKELGLKPLARIK
                     AYSSAGVDPSIMGIGPIPASRLCLKKAGWTAKDLDLLEINEAFAAQAIAVNRDMGWDT
                     AKVNVNGGAIAIGHPIGASGCRILVTLIHEMIKRDAKKGLASLCIGGGMGVALAIER"
     gene            2395..3582
                     /locus_tag="VN496_10860"
     CDS             2395..3582
                     /locus_tag="VN496_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HXF17497.1"
                     /translation="MNNGREVVVISGVRTAIGDFGGALKDFAPTELGARCVREVLTRA
                     KVAPDEVDHVVFGNVIHTETKDMYLSRVCAIHGGIPKEVPAMTLNRLCGSGLQAIVSA
                     AQFVALGDADVAVAGGAESMSRANYWLPAARWGSKMGDQIVVDSLTGALSDPFDALHM
                     GVTAENVAAKYGVTRAQQDQFSVESHLRAANAIRSGYFKQQILPIEIKDRKGTKLFDT
                     DEHVRSDATVDNLGKLKTVFKKDGTVTAGNASGINDGAAAVVLMERKRAEKRGLQPMA
                     RLVAYGHAGVEPTLMGLGPIPAVHNAMKKAGLTVDDMDVIESNEAFAAQACAVAKDLG
                     FDAKKVNPNGGAVALGHPIGATGCIISIKALYELQRIKGKYALVTMCIGGGQGIAAIY
                     ERL"
     gene            complement(3676..4407)
                     /locus_tag="VN496_10865"
     CDS             complement(3676..4407)
                     /locus_tag="VN496_10865"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HXF17498.1"
                     /translation="MEQILKVLLDAAKSMLHPIILVIVLVPMMIALALWIGIGWTYWD
                     TWSSGIRNALIAHMPSDWMSLEAARVASWLAGALVVAILTPVVILTALLIATVFAMPV
                     LVRHVAQSNFPDLQLRRGGTITGSMWNAVTAIGIFLLLWVATLPLWLLGPLAAPLPVV
                     LSAYLNQRLFRYDALSEHATAQEMKRVFARANKRLFLLGLITGLLYFIPPFNVLAPVY
                     AALAFIHLCLTELATLRASESRVPQ"
     gene            4593..5123
                     /locus_tag="VN496_10870"
     CDS             4593..5123
                     /locus_tag="VN496_10870"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXF17499.1"
                     /translation="MYNVPEQLLATNKDTLETMVEFASIAAAGAEKLLDAQMKIAKAV
                     FADAVKHAKTVVAAKDIQELTEIQTSFVQPSSERAAAYGRTLYSVVAETQSELSKFFE
                     VRFADANKNLTTALDKAMKSAPAGSDVAVAAVKSAMAAANQAYDAFSKATKQVTEATE
                     ATMMTATGTTTKKKAA"
     gene            5244..6905
                     /gene="pgi"
                     /locus_tag="VN496_10875"
     CDS             5244..6905
                     /gene="pgi"
                     /locus_tag="VN496_10875"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXF17500.1"
                     /translation="MHLIERTAWKALEAHARDTADVAILDLFAEDPHRAASFSIEACG
                     MLFDYSKNPLSAETLRRLFALAQDAGVPAQINAMFDGRRINITEKRAVLHTALRNRSN
                     RQILVGAEDVMPGIHAMLQRMRGMADRIREGIARGATGRTFTDVVNIGIGGSDLGPLM
                     VTTALRPYASKTLRAHFVSNVDGAHLAATLSGLNPETTLFIISSKTFTTQETLVNARS
                     ARQWLLTKLAGTVPAEVIVEHHFAAVSTNLDATAEFGIIPERVFGFWDWVGGRYSVWS
                     AIGLPVALAIGFDRFEEFLSGACQMDEHFRTTPLERNLPVLAALIGIWHIDFLDHRTQ
                     AVLPYAQDLVHLPAFLQQLDMESNGKRVDRSGAPVNYLTGPVIWGAPGTNGQHAFFQH
                     LHQGTQITPADFIVAVHPADDMPGHHDILLANCIAQTEALLRGKNVDEVHAELAAREM
                     QGEEVQRLVPHRIFPGNRPSNTIILDRLDPNSLGALIAFYEHKVFVQGAIWNINSFDQ
                     WGVELGKQLAGDILQDLTAGQVSDRHDPSTRMLIRHVLQRSKQQK"
     gene            complement(6947..7534)
                     /gene="orn"
                     /locus_tag="VN496_10880"
     CDS             complement(6947..7534)
                     /gene="orn"
                     /locus_tag="VN496_10880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HXF17501.1"
                     /translation="MTQRKFERTPTIMAQDPGNLIWIDMEMSGLSPDNDRVLEVAIVV
                     TDGQLNVVAEGPVQVVHQADAVFERMDSWNKATHKKSGLIDRAKAALKNEAHVEAELL
                     AFLSQHVPSNASPMCGNSVCQDRRFLARHMPKLEEYFHYRNLDVSTLKELAKRWKPGI
                     MSGFTKHGKHEALADIHESIEELKYYKLHLLNLND"
     gene            7567..8817
                     /locus_tag="VN496_10885"
     CDS             7567..8817
                     /locus_tag="VN496_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXF17502.1"
                     /translation="MNVFAILFLAALLMSTALRLWLAARHVRHIQAHRDTVPSAFATE
                     IDLAAHQKAADYSRAKTGLNMKALIFDAAVLLVLTFGGGLQAIDDLAGRVFSGAILHG
                     VAFVAMLTIIVSALELPFDLYRTFGIEGRFGFNKMTLGMFFGDLAKQAVVGAALGLPL
                     LFAVLWLMSGMGELWWLYVWIVAVAFMLFVQFIAPSVIAPLFNKFVPMQEGELKTRIE
                     NLIARCGFTSKGLFVMDGSKRSSHGNAYFTGFGNSKRIVFFDTLLSRLDIAEIEAVLA
                     HELGHFRMRHVIKRLVLMAVFSLVFFFLLGQLKSQEWFYQGLHVNYPATDAMALTLFF
                     MVMPAFTFLLQPVLSMYSRKHEFEADQYAAKFTPARDLVHALVKLYKDNASTLTPDPL
                     HSAFYDSHPPAAIRIARLEQMSHA"
     gene            complement(9000..9122)
                     /locus_tag="VN496_10890"
     CDS             complement(9000..9122)
                     /locus_tag="VN496_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17503.1"
                     /translation="MALIALVIYETVASLLRLRLFGGFRDWKIQAMAFIAAMVS"
     gene            9219..10115
                     /gene="rsgA"
                     /locus_tag="VN496_10895"
     CDS             9219..10115
                     /gene="rsgA"
                     /locus_tag="VN496_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXF17504.1"
                     /translation="MSAPHTLHHGLVVGAFGRQYLVELAGSEILSCFPRGKRSTLACG
                     DRVEIIRTAPGQGVVEAIDPRSALLYRSDQFRQKLIASNVTQVVIVLAVVPNFYEELL
                     NRCIAAAETQGLSVIIVLNKFDLAGENTGALARLTLYSDLGYRVLPLAAKRDIAPLRP
                     TLKGHASVLVGQSGMGKSTIINALIPGAGAITADISQALDSGRHTTTHARLYHLDDGA
                     DLIDSPGMQEFGLHHVTQQDLAQAFIEFRPLLGQCRFHNCRHLVEPACTIARAQEQGE
                     IDPRRLETYRKLVREIDSGKRF"
     gene            complement(10119..11045)
                     /locus_tag="VN496_10900"
     CDS             complement(10119..11045)
                     /locus_tag="VN496_10900"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HXF17505.1"
                     /translation="MSFLALLFTLLIEQVRPLTPRNAVHRAYLRYVNSLGNHFNAGER
                     KQGMAAWMLAVLPWVFVSMVIHYFLRDINIAAAWVWNVAVLYLTMGFRQFSHAFTEIV
                     EALREGNLDRARILLTEWRGESASDYDDTEIAKVAIEQGLIDSHRHVFGVIFWFLILP
                     GPSGAVLYRLSGMLEQKWGLRSAADYGDFGKFALQTFHYLDWIPVRLTAISFAIAGDF
                     EDAVYCWRSQATKWLDPEQGVVLASGAGALGVRLGETLHQHGTVSFRPDLGLGDDPDV
                     DTMTSAVGMIWRALVIWMFILALITVARWAGI"
     gene            complement(11133..11594)
                     /locus_tag="VN496_10905"
     CDS             complement(11133..11594)
                     /locus_tag="VN496_10905"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXF17506.1"
                     /translation="MKALELKIPPPIVALCVALLMWLTTRVIAPFDVPHGLRLGAALA
                     LGCVGLVLDLAGLFLFVQAKTAINPLKPGSATSLVSTGIYRATRNPMYLGLLLILLGW
                     ATFLANGLAYLIAPLFVLYINRFQIDPEERVLLQKFGPSFSAYKTKVRRWL"
     gene            11792..12259
                     /locus_tag="VN496_10910"
     CDS             11792..12259
                     /locus_tag="VN496_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HXF17507.1"
                     /translation="MQAAWIVSANAGRARFFSQANATEPLQEIHDMVNTAARLRTGET
                     ESDALGQRAASKSRHSVGAPTQPSGYEPHQSPADHQTEVFAKTVATFLSHAHQEGRFK
                     RLSLVASPDFLGVLRKLLEPTLASAVKLEINKDYTHFNARQLHERIEAHEAKR"
     gene            complement(12295..12921)
                     /locus_tag="VN496_10915"
     CDS             complement(12295..12921)
                     /locus_tag="VN496_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17508.1"
                     /translation="MKLAVFGPTGRTGRLILEQALATGHEVTAFTRHPEAVPARQGLT
                     VVAGSTNAPALLAQAIAGRDAVLCALGGHPLRRRENVCSTAMRAIVAVMQDCKVRRIV
                     AMSTFGAGETRPHVGWFARTVLFGSILRSEVADKEAMEATLSGTTLDWTVVRVGLLTD
                     DPQRMVWRAADDGSIRGMGKIARADVAAFMLAQLHHSPWVRRSPVIMY"
     gene            complement(13146..14420)
                     /locus_tag="VN496_10920"
     CDS             complement(13146..14420)
                     /locus_tag="VN496_10920"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF17509.1"
                     /translation="MKIVRRLVGSLGLLCTVNIAAAGDLPSAMPEEMGFSSQRLARIG
                     PVIQGEIEKGQYPGAVILVARKGKIIYFEGFGQLDPTSARPMAKDAIFRLYSMTKPYV
                     SVAAMMLMEEGRLRVTDAVSKYIPALSKLEVSVASTDPYTGAVKYTNVPLDREITIQD
                     LLRHTSGFVYGAFTSHPMVKELYAKEGVDWKEVTPAEQIDRLAKVPLAHQPGTTFEYG
                     LSTDVLGRVIEAITGMTLGDFLQERIFTPLAMSDSAFIVPREKRDRLAQPFAIDRATK
                     NPINLLDVTVQQKNDAGGAGTVGTASDYARFLQMMINGGQLDGVRLLSRTTVAYMTSD
                     HLGDAKTSGATTLPAGTGFGLGFAVRRQTGLFEVTGSAGEYFWAGAAGTGFYVDPKEE
                     LICVWMTQSQPGMPRRYDRYLFKQMVYQAIVD"
     gene            complement(14552..>15442)
                     /locus_tag="VN496_10925"
     CDS             complement(14552..>15442)
                     /locus_tag="VN496_10925"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF17510.1"
                     /translation="YVFNRGASTMQTSYQSASMSKMIATVIILRQVELGVLSLASRPQ
                     DFIRTWPISGSDPLAGMTLTQLLSLTSGLSTDVGCMDSATANFETCVNTIASSNIGNG
                     MAAGQQFSYADAHHQVAALMAVKARGMAGWQDLFAEFQTQTGLFPNTTFDIPSASNPR
                     IAGGMHITAGDYMDFLAKLKSGALLNASSMSDMLADHIVSLPIKFSPIFSGLNGGPGL
                     GEDWHYGFGLWQECQSTQYNCVPGSRVSSPGHFGGYPFWDRDKSYTGVVMRQGTSSTV
                     INGIKIERTVRPDVERWAAC"
BASE COUNT         3489 a         4401 c         4347 g         3205 t
ORIGIN      
        1 gtatcaaggc atcgtcagca tagaaggcga cagtatcgcg caggtgctcg agcattacat
       61 ggcgaaatcg gagcaacttg aaacccggct gtggctagcg tccgactcca gccaggccgc
      121 cgggatgctg ttgcagaaac tgcccgcgac actgagcgaa gatgcggacg cttggaaccg
      181 cgccactcgg ctgggcgaaa caatcaagcc ggaggaattg ctcagcttac cggctcccga
      241 gatcattcgt cgcctatatc atcaggaaga tgtccgggta ttcgagccgc ggccagtggc
      301 atttcgctgc tcatgctcgc atgaccgcgt tacctcgatg ttgcgcatgc ttggacacga
      361 cgaagtgcgt tccatcatcg aagaacgcgg taacgtggag gtcgactgcg aattctgcgg
      421 acgccactat gtttttgacg ccgtcgatgc cgaacaggta ttcgcggcgg atgtcatcct
      481 gccggtagag aaaaccaggc attgataatc cactccaatg agtgaatcac agacccgtgt
      541 acgcattgac aagtggctat gggcgggcgc tccggactcg ttggcgacgg gtcgcccgtt
      601 tgcgggcagg ggcgcttcgg ggccgccagc cgagcgctta ctgagatgag tgaatcagat
      661 acgcgtgtac gcatcgacaa gtggctatgg gcggcccgct tctacaaaac ccgcagcctg
      721 gcagccgacg cggtagattc cggcaaggcg aagctcaatg gcgaacgcgt gaaaccggca
      781 aagatgctga agaccggaga tacgctgaac attcgcaatg gacttttaag ctgggaggtg
      841 accgtgcttt tgctgagtga ccggcgcggt tccgccatgg tcgcgtccac actgtattcg
      901 gaatcagacg acacccgaag gcaacgggaa caacagagcg caatgctcaa ggctgagcgc
      961 caggcaaacc cgttccctgg cggacgtccg accaaacggg cccgccggca gatcatccga
     1021 ttcatacgcg aacaggagtg atcgtgtccc tatatttgca ataccgacgt cgggtttcaa
     1081 ataacggttt ttcctcgaca ggtttcaaaa cccgggcgta aactgcgccc tccgtcgtgc
     1141 tgcctctgaa gacaatctcc ggccacgatg ctgtcctgga tattcatgta ggagaatttc
     1201 aagatggatg acgttgtaat cgtcgctgca ttgcgcactg gtgtcgggaa atttggtggc
     1261 acgataggca agatccccgc aactgaactc ggtgcgaaag taatcaccgc attgcttgct
     1321 aggacgggcg taggaccgga actggtgagc gaggtaataa tgggccaggt gcttacagca
     1381 gccgcgggcc aaaatcccgc ccgacaggcg tcgatcatgg caggggtccc gaatacggtt
     1441 cccgcaatga cgattaacaa agtgtgcgga tcgggactca aggcgacgca tctggccgcg
     1501 caagcaatca aggccggtga cgccgatatc gttattgcag gcggacagga gagcatgaca
     1561 cttgcgcctc acgcgctcaa tggagtgcgt gacgggttgc gaatgatgga tggcaagctc
     1621 gtcgacacga tgatcattga tggtctttgg gacgtctaca accaatacca catgggtgtc
     1681 acggcggaga atgtcgcgaa gaaatggaat gtatcgcgac aggaacagga cgagttcgcc
     1741 gcggcttcac agcaaaaagc agaggcggca cagaaggcgg gccgtttccg ggatgaaatt
     1801 gttcctgtga ccatttccac ccgcaaaggc gatgtccttt tcgacaccga tgaatacgtg
     1861 cggcacggaa ctaccgtgga ggcgctggct tcgctgaagc cagcatttag taatagcgga
     1921 actgttacgg caggcaatgc ttccggccta aacgatggcg cagcggcagt aatgatgatg
     1981 agcaccgcga aagcaaagga actgggacta aagccgcttg cgcgcatcaa agcctattca
     2041 tcggccggcg ttgatccaag catcatgggc atcgggccga tacccgcgtc caggctatgc
     2101 ctgaaaaagg ccggatggac ggcgaaagat ctggacctcc tcgaaatcaa tgaagctttt
     2161 gcggcacaag ctatcgccgt gaatcgcgac atgggctggg atactgcgaa agtcaatgtg
     2221 aacggcggcg caattgcgat cgggcatccc atcggagcgt ccggctgccg catcctcgtc
     2281 acgctgattc acgaaatgat caagcgcgat gcgaaaaaag gtctggcgtc gctatgcata
     2341 ggcggcggca tgggggttgc tctggcaatt gaacgctaga aatcaaggat tatcatgaac
     2401 aacggacgtg aagtcgtagt gatctcggga gtcagaaccg ccatcggcga tttcggcgga
     2461 gcactaaagg atttcgcacc cacggaattg ggtgcacgct gcgtgcgtga agtgctgacc
     2521 cgagccaaag tcgcaccgga tgaagtggac catgtcgttt tcggtaacgt cattcatacc
     2581 gaaacaaaag atatgtacct gtcgcgcgtc tgcgcgattc acggtggaat cccgaaagag
     2641 gttcccgcga tgacattgaa ccgactgtgc ggcagcggat tgcaggcgat tgtcagtgct
     2701 gcgcaattcg ttgcccttgg ggatgcggac gttgcagttg caggcggtgc tgaatccatg
     2761 agccgagcca attactggtt acccgcggcg cggtggggat cgaagatggg ggaccagatc
     2821 gtggtcgact cactcaccgg ggcgctgtct gatccgttcg atgccctgca catgggcgtc
     2881 actgcggaaa acgtggccgc gaagtatggc gtcacgcggg cccagcaaga ccagttttcc
     2941 gttgaaagcc atctccgggc cgctaatgcg atccgcagcg ggtacttcaa gcagcagatc
     3001 ctgcctatag aaatcaagga ccgcaaaggc accaagctat tcgataccga cgaacacgtg
     3061 cgcagcgatg cgactgtcga caatctgggc aaactgaaga cagtcttcaa aaaagacggc
     3121 acggtgactg caggaaatgc ttccggcatt aacgacggcg cagcggcggt ggttttgatg
     3181 gagcgcaaac gcgcggaaaa acggggtttg cagccgatgg cgcgcctggt tgcttacggg
     3241 cacgctggcg ttgaaccgac cctgatgggt cttggcccga tcccagcagt tcataacgcc
     3301 atgaaaaaag caggccttac tgtggatgac atggacgtga tcgagtctaa tgaagcgttc
     3361 gctgcgcaag cttgcgctgt ggccaaggat cttggcttcg acgcaaaaaa ggttaatccg
     3421 aacggaggcg cggttgcgtt gggtcacccg ataggggcta ccgggtgcat catttcgatc
     3481 aaagcactgt atgagctgca gcgcatcaag ggaaaatacg cgctggtcac catgtgcatc
     3541 ggcggtggac aagggattgc tgcaatatac gaaagactgt agatagcatt ttcctcgtta
     3601 cgcgacgtcc tgcgccgcgc tttggttttt caccacgtcc ttatctcgcg tgctctttgt
     3661 cacggttcta gaaacttatt ggggaacgcg agactccgaa gcccgaagtg tcgccagctc
     3721 tgtgaggcac aggtgaataa aagccagcgc tgcatagacc ggtgctagca cattaaacgg
     3781 cggaatgaaa tacagcagcc cggttatcaa gcctagtagg aacaagcgtt tgttggcacg
     3841 cgcgaatacc cgcttcatct cttgtgcagt agcatgctct gacaaagcgt cataacgaaa
     3901 cagccgttgg ttaagatagg ccgataagac gacaggcaaa ggagcggcca gcggcccgag
     3961 cagccacagt ggcagcgtcg caacccatag cagcaaaaat atgccaatgg ctgtcaccgc
     4021 attccacata ctgccggtaa tcgtgccgcc gcggcgcaac tggaggtctg gaaaattgct
     4081 ttgcgcaaca tgtcggacaa gaacgggcat ggcaaaaacc gttgcgatta gaagcgcagt
     4141 caagataaca accggggtca atatcgcaac gacaagcgca cctgccaacc acgaagccac
     4201 cctggcggct tccagggaca tccagtcgga tggcatgtga gctatcagcg cgttccgtat
     4261 gccagagctc caagtgtccc agtaggtcca tccaatgccg atccagagcg ccaatgcaat
     4321 catcatggga accagcacga taaccagaat gatgggatgc aacatggact tagcggcatc
     4381 caacagtacc tttaatattt gttccaccag agcctctcgc tattggatag gctatttaac
     4441 tgactgtttg taggaaattt cttacgagcc gggaccgcag gaccaaaagc aaaattcttg
     4501 ttgacttcgc tataaagccc gatagaatgg cgacgcttat tgcagtgcaa caacattaca
     4561 taccccaacc ctgcctgcct gaaggagaag tcatgtacaa cgtacctgaa caactgttag
     4621 caacgaacaa agacaccttg gaaacaatgg tggaatttgc cagcatcgca gcggcgggtg
     4681 ccgaaaagct gctggacgca caaatgaaaa ttgcaaaagc tgtatttgcg gatgccgtaa
     4741 agcatgccaa aacagttgtg gccgccaagg acatccagga actgaccgaa atccagacat
     4801 catttgtaca gccgtcttcc gaaagagctg ctgcctatgg ccgcaccttg tattcggttg
     4861 tcgctgagac ccaatccgaa ctgagcaagt tcttcgaagt gcgtttcgcc gatgccaaca
     4921 agaacttgac cacagcattg gacaaagcca tgaaaagcgc tccggccgga tcggatgtcg
     4981 cagtagcagc ggtcaagtca gcaatggctg ccgctaacca ggcgtacgac gcattcagca
     5041 aggcaaccaa gcaagttacc gaagccaccg aagccacgat gatgacagcc accggcacga
     5101 caacgaagaa aaaggccgct taagcagcca gcaccgcgct ccttacattg aaggcccacg
     5161 gatcgatccg tgggcctttt tgtttttgga cgctctctat agaatcagac tagttgtcgc
     5221 agctcccgcc aggtcgtcat tgcatgcatc tcatcgaacg aaccgcatgg aaggcgttag
     5281 aagcgcacgc ccgcgacacc gcggacgtcg ccattcttga tttgttcgcc gaagacccgc
     5341 accgcgccgc atcgttttca atcgaggctt gcggcatgct attcgattat tcgaagaatc
     5401 cgctgtcggc tgaaaccctg cgacggctgt ttgcattggc gcaggacgcc ggtgttccgg
     5461 cacaaatcaa tgcaatgttc gacggccggc gcatcaacat taccgagaag cgcgctgtgt
     5521 tgcataccgc cttacgcaac agatcaaacc gccagattct ggtaggcgca gaggacgtca
     5581 tgccgggcat tcatgccatg ctgcagcgca tgcgtggcat ggccgatcgt atccgggaag
     5641 gcattgcgcg gggcgcaacc ggccgaacct ttactgatgt ggtcaatatc ggcatcgggg
     5701 gctcggatct ggggccgtta atggtaacga cagccttgcg cccttacgct tccaagaccc
     5761 tgcgtgcgca ttttgtgtct aacgtggacg gcgcgcacct ggccgcaacg ctttcgggcc
     5821 tgaatccgga aacgacgctc ttcatcattt cgtcaaaaac ctttacgaca caagagacgc
     5881 ttgttaatgc cagatcggca cgacagtggt tgcttactaa attggccgga acagtccctg
     5941 ctgaagtcat cgtcgagcac catttcgcgg ccgtttcgac aaacctggat gccactgccg
     6001 aatttggcat catcccagag cgcgtgtttg gattttggga ctgggtgggt ggccgctact
     6061 cggtctggtc agccattggt ctgccagttg cgcttgcaat cgggttcgac cgtttcgagg
     6121 aatttctcag cggcgcctgt caaatggacg agcacttccg gaccacgccg cttgagcgca
     6181 acttgccggt gttggccgcg ctgatcggta tttggcacat cgacttcctt gaccaccgca
     6241 cccaggcggt gctgccttat gctcaggatc tcgtacatct tccggctttc ctgcagcaac
     6301 tcgacatgga aagcaatggc aagcgcgtgg atcgctcggg ggcgccggta aattacctta
     6361 cgggaccggt catctggggt gcacccggga ccaatggaca gcatgcgttc ttccagcatc
     6421 tgcaccaagg gacgcagatc accccggctg attttattgt ggctgtccac cccgcggacg
     6481 acatgcccgg tcatcatgac atcctgctgg cgaactgcat tgcccagacg gaagcgctgt
     6541 tgcgcggaaa aaacgttgat gaagtccatg ccgagctcgc cgcccgcgag atgcagggtg
     6601 aagaagtgca acgattggta ccccatcgca tatttccggg caaccgtccc agcaatacca
     6661 tcatcctcga tcgactcgac ccgaattcgc tcggtgcctt gatcgcgttt tatgaacaca
     6721 aggtttttgt gcagggcgcg atctggaata tcaattcatt cgatcaatgg ggcgtggaac
     6781 taggtaagca acttgccggt gacattctcc aggatctcac ggcaggacag gtctcagacc
     6841 gacatgatcc ttcgaccagg atgttgatcc gccacgtact gcagcggagc aaacagcaaa
     6901 aatagagtcg gatacccggc agtacgcata tttgccagaa ggactctcag tcgttcagat
     6961 taagcaagtg caatttgtag tactttagtt cctcgatcga ttcatggatg tcagccaacg
     7021 cctcgtgttt tccgtgcttg gtgaatcctg acatgatccc gggcttccaa cgcttggcca
     7081 gttccttcag cgtggagaca tcaaggttgc ggtagtgaaa atattcttcc agcttaggca
     7141 tgtgccgggc gaggaagcgg cggtcctggc acaccgagtt accgcacatc ggcgaggcgt
     7201 ttgacggaac atgctgtgac aggaacgcga gcaattccgc ctccacgtga gcctcattct
     7261 ttagcgccgc cttggcacga tcgatcaggc cggacttctt gtgcgtcgct ttgttccagg
     7321 agtccatgcg ctcgaatacc gcgtcggcct gatggacgac ttgtaccggg ccttccgcca
     7381 cgacgttcag ttgcccatca gtcacgacga ttgccacttc gagtacccgg tcattgtcag
     7441 gactaagccc gctcatttcc atgtcgatcc agatcaggtt gccgggatcc tgtgccataa
     7501 tcgtcggagt tctctcgaat tttcgctgcg tcattttgtc acagatccaa accccaaccg
     7561 acctccatga acgttttcgc gatccttttt cttgcagcat tgctgatgtc cacggctctg
     7621 cgtctatggt tggctgcacg acatgtgcgc cacatccagg cccatcgcga tacggttcca
     7681 tccgcgttcg cgaccgaaat agatctggcg gcccatcaga aggcagctga ctacagtcgc
     7741 gccaaaacgg gcctgaacat gaaagcgctc atctttgatg cggccgtgtt gcttgtgctg
     7801 acttttggtg gcgggctgca ggcgattgat gaccttgcgg gccgtgtttt ttccggcgcc
     7861 attttgcacg gtgttgcctt tgtcgcgatg ttgacgatca tcgtgtctgc gctggagttg
     7921 ccctttgatc tctatcgcac tttcggcatc gagggccgct ttggcttcaa caaaatgacg
     7981 ttgggcatgt tcttcggcga ccttgccaaa caagccgtcg tcggtgccgc gcttggattg
     8041 ccgctgctgt ttgccgtgct gtggctgatg tccggtatgg gagaactgtg gtggttatat
     8101 gtctggatcg tggcggtcgc cttcatgctg ttcgtccaat tcattgcccc gagtgttatt
     8161 gcgccgctgt tcaacaaatt cgtcccgatg caagagggag aactaaaaac acgtatcgag
     8221 aatctcatcg cgcgatgcgg gttcacctcc aaaggtttgt tcgtgatgga tggctcgaaa
     8281 cgaagcagcc atggcaacgc ttatttcacc ggtttcggca actccaagcg catcgtcttt
     8341 tttgatacgc tgctgtcgcg tctggacatt gccgaaatcg aagccgtgct ggctcacgag
     8401 cttgggcatt ttcgcatgcg ccacgtgatc aagcggctgg tattgatggc cgtgttttca
     8461 ctggtcttct tctttctgct cgggcagctc aagagccagg aatggttcta tcagggactg
     8521 cacgtgaact atccggccac agatgcgatg gcactgacgt tgtttttcat ggtaatgccg
     8581 gccttcacgt ttttgctgca gcccgtgctt tcgatgtatt cccgcaagca tgaattcgaa
     8641 gcggatcagt acgccgcaaa atttacgccg gctcgtgatc tggttcatgc attggtaaaa
     8701 ctttacaagg acaacgcctc caccctgact ccagacccgc tgcattctgc cttttacgat
     8761 tcccatccgc cggcggcgat tcggatcgct cggctcgagc agatgagcca cgcctgaaga
     8821 gcacgctatt cgggtgattc tcggggaatg cggcatcagt gaaccgctcg aagtccggca
     8881 cgcaagaaat gcaaccctgt gaaggccgcg tcatccgcag gagcgagtgg aacacgtccg
     8941 ccattccgga tggcagtacc aggcggctca ggtgcaaagc gtttcgagct cagaaattat
     9001 tatgagacca tggccgcaat gaatgccatc gcctggattt tccaatcgcg gaagccgcca
     9061 aacaatcgca gtcgcaacaa cgaagcgacg gtttcataga tgaccaatgc aatcaatgcc
     9121 atctctgaaa acggcttcgt ctgtgcgtgc gatcaggata gctgcactgt tcgctgtcta
     9181 acgtcaccgc tgaagtctgg catggcgatg aataactttt gagcgcgccg cacacgctgc
     9241 atcatggtct ggtcgtcggc gcatttggcc ggcaatatct ggtcgaactc gccggttcag
     9301 aaatcctcag ctgtttcccg cgcggcaagc gcagcacgct cgcgtgcggc gaccgtgtgg
     9361 aaatcatccg tacagcgccg ggccagggtg tggtagaagc catagacccg cgttcggcgt
     9421 tgctgtatcg atcggatcag ttccgccaga aactgatcgc atccaatgta acgcaggtcg
     9481 ttatcgtcct tgccgtggtt ccgaattttt atgaagaatt gctgaatcgc tgcatagcgg
     9541 ccgccgagac gcaggggcta tcagtcatca tcgtactgaa caagttcgac ctggctggag
     9601 aaaatacggg tgctcttgct cgccttacgc tgtattccga cctcggctat cgcgtcctgc
     9661 cgcttgcggc caagcgtgac atcgcgccac tgcgtcccac actgaaaggc catgcgagcg
     9721 tgctggtggg acaatcgggc atggggaaat caaccatcat taatgcgctg attcccgggg
     9781 ccggcgccat aacggcagat atttcgcaag cgctcgactc cggacgccat accacgactc
     9841 acgcgcggct gtatcacttg gacgatggcg cggatctgat cgattctccc ggcatgcagg
     9901 agttcggttt gcatcatgtc acgcaacagg acctggcgca ggcgttcatc gagttcaggc
     9961 cgttgctcgg ccaatgccgc tttcacaatt gcaggcacct cgttgaaccc gcctgcacca
    10021 tcgcgcgcgc gcaggagcaa ggcgaaatag atccgcggcg gcttgaaacc taccgcaagc
    10081 tggtgcggga aatcgattcc ggaaagcggt tctagcgctt agatacccgc ccaacgcgct
    10141 acggttatca acgcgaggat gaacatccag atcacgagcg cgcgccagat catgcctacc
    10201 gcactggtca tggtatccac gtccgggtcg tctcccagcc cgagatcggg acggaaactc
    10261 accgtgccat gctgatgcaa cgtctcgccc aggcgaacgc cgagagcacc cgccccgctc
    10321 gccaacacta cgccttgttc cggatccagc catttggtgg cctgcgatcg ccagcaatac
    10381 acggcgtctt caaaatcgcc ggcgatggca aagctgattg cggtaagccg cacgggaatc
    10441 cagtcgaggt agtggaatgt ctgcaaagca aatttgccaa aatcaccgta gtcggccgcg
    10501 gagcgcaggc cccacttctg ttcaagcatt ccgctaaggc ggtacagcac cgcaccgctc
    10561 ggacccggca ggataaggaa ccagaaaata acgccgaaca catggcgatg cgaatcgatc
    10621 aagccctgtt caatcgcgac cttcgcgatt tccgtatcgt cgtagtcgga cgcagactca
    10681 ccgcgccact cagtcaataa aatacgggcc cggtccaggt tgccctcgcg caatgcctct
    10741 acgatttcgg tgaacgcgtg gctgaactgg cgaaagccca ttgtcagata aagcaccgca
    10801 acgttccata cccatgccgc cgcaatattg atgtcccgga gaaaataatg gatgaccatc
    10861 gacacgaaca cccacggaag gaccgcaagc atccaggccg ccatgccttg cttccgttca
    10921 ccggcattga aatggttacc aagcgaattg acatagcgca aatacgcacg gtgcacagcg
    10981 ttgcgcggcg ttagcgggcg gacctgttcg atcagaagcg taaacagcag ggcaagaaag
    11041 gacattgctg aacccgcgtt ttttgattag tgacatctta ccggatcatg gcttccaggc
    11101 cgcctccaca atgatccacg gcccggcact acctatagcc agcggcgaac ctttgtcttg
    11161 taggcagaga aactcgggcc gaatttctgc aacagcacgc gttcttcagg atcgatctga
    11221 aagcggttga tgtagagcac gaatagcggc gcaatcaaat atgccagccc attggccagg
    11281 aatgtcgccc acccgagcaa tataagcaga aggccgagat acatcggatt gcgcgtcgcg
    11341 cggtagattc ccgtgctaac cagcgacgtt gcggagccag gcttcagcgg attaatggcc
    11401 gttttggctt gcacgaataa aaacaggccg gccaggtcca gcacaagacc cacacaaccg
    11461 agcgccagcg ctgcaccgag gcgcagccca tgcggcacat caaaaggcgc aatcacccga
    11521 gtcgtcagcc acatcagcag cgccacgcaa agcgcgacga tgggcggcgg gattttgagc
    11581 tcaagggctt tcactggaac aaacgccttt catcgaggcc atcctgcgtt gttccgcgcg
    11641 cgtgcaatcc agagcaagag ccattcgacg atggcattga gctagtccct gctacccctg
    11701 atctggcttt caatggcatt tcgcctgaac aacggaacac tttcgtggtc gagacatcga
    11761 acgatttcca actctatatt gagaggccaa catgcaagcc gcctggatcg tgtcagcgaa
    11821 tgcagggcgt gcgcggttct tttctcaagc aaacgcgacg gaacctttgc aagaaatcca
    11881 cgatatggta aacactgccg cccgtctgcg cacgggggaa accgagtccg atgcgctggg
    11941 ccagcgtgct gcttcaaaaa gccggcacag cgtcggggcc ccaacacagc cgagcggtta
    12001 tgagccccac caatcgcccg ccgaccacca gacagaagtg ttcgcgaaga cggtagcgac
    12061 ctttctctcg catgcgcatc aggaaggccg gttcaagcgg ctgtcgctcg ttgcctctcc
    12121 ggacttcctg ggtgtgttgc gcaaactgct ggagcccacc ctggcatcgg cggtaaagct
    12181 ggagatcaac aaggactaca cccacttcaa cgcccggcaa ttgcatgagc gtatcgaggc
    12241 gcatgaggca aaacgctagc gcgcccttcg gactcgccgt gcgtttcggc agccctagta
    12301 catgatcacc gggcttcgcc ggacccaggg gctgtgatgc aactgtgcaa gcatgaaagc
    12361 ggcaacatcc gctctcgcaa tttttcccat tccccgaatt gatccatcgt ccgctgcccg
    12421 ccagaccatc cgctgcggat catcagtcag caacccgacg cgcaccaccg tccagtccag
    12481 cgtggttccg gatagcgtcg cttccatcgc ttccttgtct gccacttcgc tgcgcaatat
    12541 cgaaccgaac agcacggttc gcgcgaacca accgacatgc ggacgcgttt cacccgctcc
    12601 gaacgttgac atggcaacga ttcgccgaac cttgcagtcc tgcattaccg ccacgatggc
    12661 tcgcattgca gtcgagcaga cgttctcacg tcggcgcaac gggtgcccgc cgagcgcaca
    12721 cagtacagca tcgcgcccgg cgatcgcttg cgccagcaaa gcgggcgcat tagtactacc
    12781 ggcgaccacc gtgagcccct ggcgcgccgg caccgcttcc ggatggcgag tgaatgcggt
    12841 cacttcgtga cctgtcgcaa gcgcctgctc gaggatgagc cggccggtgc ggccggtggg
    12901 accaaagaca gcgagtttca ttcattgtcc gaaagaacga ttggaatcct agtcggcttg
    12961 catccccgat gcatcattag cgcaatgcgg gacgcttttc atgccgcctc attacttgtt
    13021 gcagacggcg accaaatcgt ccgcttacaa ggaattggtc aacaagcgtt cctactggca
    13081 gccgtggact ccgccattca agggccggct gccggttcgg acgcaacaaa ctttgtcgcg
    13141 gctgctcagt caacgatggc ctgatatacc atttgcttga acagatagcg gtcgtagcgg
    13201 cgcggcatgc caggctggct ttgcgtcatc cacacgcaga tgagttcttc tttcgggtcg
    13261 acgtaaaatc cggttccggc agcacctgcc cagaagtatt cgccggcgga gcccgtgact
    13321 tcgaacaggc ccgtttggcg ccgtaccgca aagccgaggc cgaagccggt gccggccggc
    13381 aacgtggtgg caccggacgt tttggcatcg ccgaggtgat cggacgtcat gtacgccacc
    13441 gtggtacgac tgagcaaccg cacgccatcg agctgaccgc cgttgatcat catctgcaag
    13501 aaccgcgcat aatccgatgc ggtgcccaca gtgcccgctc caccggcatc gttcttttgc
    13561 tggaccgtga catcgagcag attgattgga tttttggtcg cgcgatcaat cgcgaacggc
    13621 tgtgccagac ggtctcgctt ctcgcgcggc acaataaatg cggaatcgct cattgcgagg
    13681 ggcgtgaaaa tccgctcttg caggaaatcg ccaagcgtca tgccggttat cgcttcgatc
    13741 acgcggccga gaacatcggt ggacaggccg tattcgaaag tcgtgcccgg ctgatgagca
    13801 agcggaactt ttgcaaggcg gtcaatctgc tctgccggtg tcacctcttt ccagtcgacg
    13861 ccttctttgg catataactc cttcaccatg ggatgcgatg tgaaagcccc gtacacaaat
    13921 cccgatgtat gccgcaaaag atcctgaatc gtaatttccc gatcgagcgg aacgttcgta
    13981 tatttcacgg cgccggtgta cggatctgtt gatgcgacac tgacttcgag cttcgataag
    14041 gccggaatat attttgaaac ggcgtccgtc acgcgcaagc gtccttcttc catcagcatc
    14101 atggccgcca cagacacgta aggcttggtc atcgaataga ggcgaaagat ggcatctttg
    14161 gccatcggcc tggcgctcgt tggatccagc tggccaaaac cctcgaaata aatgattttt
    14221 cctttgcgcg cgaccagaat gacagcgccc ggatattgcc ccttctcaat ctcgccctga
    14281 atgaccgggc caatgcgggc gaggcgctgg gaagagaatc ccatttcttc gggcattgcc
    14341 gatggcaaat cgcccgccgc tgcaatattt acggtgcaca acaaaccaag gctgccgacc
    14401 aaccggcgaa cgatcttcat tgcttatctc cctgtacggc ccttgaacga cggcccttga
    14461 atgtaggcgt tgtcccgcgc gttggcaatg gaaccttcac tatttggcag gcgctcatcg
    14521 cggcttgcag aaccgtacga cgggagtcgg ctcaacaagc ggcccagcgc tcgacgtcag
    14581 ggcgcacggt tcgctcgatc ttgatgccat tgatcacggt ggaggaggta ccttggcgca
    14641 tgacaactcc cgtataggat ttgtcacggt cccaaaacgg gtagcccccg aagtggccgg
    14701 gactcgatac cctgcttccg ggaacgcagt tgtattgcgt gctttgacat tcctgccaga
    14761 gcccgaaacc atagtgccag tcttcgccca gaccgggacc tccatttagc cccgagaata
    14821 tcggcgagaa ttttatcggg agtgagacga tgtgatccgc cagcatatcg ctcatggacg
    14881 atgcgttcag caaagcaccg cttttcagct tggcgagaaa atccatgtag tcgccggcag
    14941 tgatgtgcat gccgcccgca atgcgcggat tgcttgctga cgggatatca aacgtggtgt
    15001 tgggaaagag tccggtctga gtctggaatt cggcgaacag atcctgccag cccgccatgc
    15061 cgcgcgcctt tacggccatc agcgccgcga cctggtgatg cgcgtcggcg tacgagaact
    15121 gctggccagc cgccatgcca ttgccgatgt tgctgctggc gatggtattg acgcacgttt
    15181 cgaaattagc cgtcgccgaa tccatgcatc ccacatcggt tgacaagccg gatgtgaggc
    15241 tcagcaactg tgttagtgtc atgcccgcca gcggatcact gccggaaatg ggccatgtgc
    15301 gaataaaatc ctggggtctt gaagcgagcg acagaacgcc cagctccacc tggcgcaaaa
    15361 tgatgacggt cgcaatcatt tttgacatcg atgcggactg ataggaggtc tgcatcgttg
    15421 atgcaccgcg gttgaacaca ta
//
LOCUS       DATMZQ010000343         5805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1316605, whole genome shotgun sequence.
ACCESSION   DATMZQ010000343 DATMZQ010000000
VERSION     DATMZQ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316605"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1186
                     /locus_tag="VN496_10930"
     CDS             83..1186
                     /locus_tag="VN496_10930"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXF17511.1"
                     /translation="MSQLAHTAPVDLKPPIEPMLAKLADALPAGENYLYEPKWDGFRA
                     IVFREGADVFIQSRDLRPLDRYFPELHDDLLKQLPQGCVLDGEIVIATARGLDFDALQ
                     LRLHPAASRVAKLAQQTPSAFVAFDALAIAGESLMPKPQSERRTALERLLAKVKPPVY
                     LTPMTQDRSIALEWLARFEGAGLDGVVAKPARLPYQPGQRAMIKVKHARTADCVVAGY
                     RVYKSSDDAVGSLLLGLYDDQNNLHHVGVTSSFTMPVRQQLMKELAPWRANALANHPW
                     RSWAQATEGSTRMPGAQSRWSAGKDLSWEPLRAELVCEVKYDHLQGDRFRHAATFLRW
                     RSDKPAADCRYDQLAVTRPYELAKVFEPTQQAR"
     gene            complement(1131..2186)
                     /gene="ligD"
                     /locus_tag="VN496_10935"
     CDS             complement(1131..2186)
                     /gene="ligD"
                     /locus_tag="VN496_10935"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXF17512.1"
                     /translation="MAKATHELVRIDGVEIPVSNPQKVLFPEAGYTKLDVVRYYMEVS
                     SGALRGAGGRPNMMVRYPNGVNGEFFYQKRAPASRPEWIDVVEIKFPSGRSADEIVPR
                     HAAALVWMANLGCLELHPHPVRANDLEHPDELRVDLDPVPGIEWPQVREVAYVCQHVL
                     QEFGLVGWPKTSGKRGLHIYVRIKSRWTYDQVRRAALAFGREVERRCPDIATTKWWKE
                     ERKGVFIDYNQNAKDRTIAAAYSIRPTADARVSAPLAWEELRDAVPADFTLKTMPARF
                     RKLGDRHENIDREKGSLDSLLELSARQERDGEGDAPWPPQYKKRAGEPIRAQPSRRRI
                     SAPAASARRLSPVRKGA"
     gene            complement(2224..4434)
                     /locus_tag="VN496_10940"
     CDS             complement(2224..4434)
                     /locus_tag="VN496_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXF17513.1"
                     /translation="MIHTGLIGRPIDRVDGPYKVTGRARYAAEFFPPDVAYGVLVLST
                     VPSGQVRIDTGTVRSMPGVLGVLTHDNVASLPQKGRSAYGPPAGRTMSLLQDDEVHYN
                     GEPVAVVVAETFEQATEAAAKLDVTYVTRAAQLSFDAAKSVAHKPEKLARGTEPDLNW
                     GDVDAGLRAADVVIENVYTTPMEHHNPIEPHGTVAHWENDRLTLYDATQYVSGVRQTV
                     SKTLGIEVEKVRVIDPYVGGGFGCKGSTWSHVVLAALAAKQVQRPVKLVLSRPQMFGP
                     VGGRPQTEQRLVLGAARDGKLTGLRHAVISHTSVMEDFAEACTNPTRSLYACANGAVT
                     QRLAKLNVGVPTFQRAPGESTGSFAIESALDELAYALGMDPLDLRLRNYAEAEPSTGK
                     PWSSKQLRACYSLAAERFGWARRTREPRSMRDGRWLVGWGMATATYPGHRMTASAVAR
                     MLPGGNVLVQSGTQDLGTGTYTIMTQIAAQSLGVAVERVRFVLGDTALPQAPVSGGSM
                     TAASVGPAVQNACVALRDKLIALSVADPASPLHGRAAVDATIENGWIVAREGSARESV
                     SDLVGRQRSGVEARGDAKPGEEEKQFASRSFGAVFAEVRVDEQTAMLQVQRISAVYSV
                     GKVMNEKTARSQLEGGIVWGLGMALFEHSVLDPRYGRFVNANLAEYHVPVNADVRKIE
                     VDFVTEDDQVFNPLGGRGIGEIGITGVPGAVANAVFHATGKRIRDLPITLDKLI"
     gene            complement(4431..5417)
                     /locus_tag="VN496_10945"
     CDS             complement(4431..5417)
                     /locus_tag="VN496_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXF17514.1"
                     /translation="MREFEYERAEQIEEAIAAALAPQTKYLAGGTNLLDLMKGDVERP
                     VRIVDINRLPLAQIVQYPNGGVRIGAMVRNSDVADHPYIREHYPLLSQALLAGASAQL
                     RNMASVGGNLLQRTRCYYFYDTAFPSCNKRDPGTGCAARDGFNRIHAIFGASEHCVAT
                     HPSDMAVALAALDAVVEVRGHDGTRRIPIDQFYRLPEDIPAIDTTLAPGELILAVELP
                     PSRFSAHSHYLKVRDRSSYAFALVSVAAALHQQDGKVIDARIVLGGVAHKPWRVKNAE
                     RQLIGSPLDGGAIEAAARASVEGAQPLQDNAFKIDLARRAVVQALQVAEASS"
     gene            complement(5430..>5805)
                     /locus_tag="VN496_10950"
     CDS             complement(5430..>5805)
                     /locus_tag="VN496_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXF17515.1"
                     /translation="GACTVLVNGRRVNSCLSLAVSHDGDEVVTIEGLGVPEAMHPLQR
                     AFVQHDAYQCGYCTPGQVCSAVGLLAEANAGAASAVTPDVRVNGPARLTDSEIRERMS
                     GNLCRCGAYVNIVAAIRSIAQS"
BASE COUNT         1122 a         1816 c         1734 g         1133 t
ORIGIN      
        1 taaacattac gcatagcgag cgggtcaacg atggctagtg aactgcacat ttgctttgcc
       61 gcgcggtcga aggtgcatga gcatgagcca attggcacac accgccccgg tagacctaaa
      121 gccgccaata gaaccgatgc tggccaagct cgccgatgcg ctgccagctg gcgaaaatta
      181 tctgtacgaa cccaagtggg atggtttccg cgccattgtg tttcgcgaag gcgcagatgt
      241 attcatccag agccgggatt tgcgcccgct cgatcgttac tttccggagt tgcacgacga
      301 cttgctgaaa cagctgccgc aaggctgcgt actcgacggc gagatcgtca tcgctacggc
      361 gcgcggactt gatttcgatg cattgcagct gcgcttgcat cctgccgcat cgcgcgtagc
      421 gaaactcgcg caacaaacac cctcggcatt cgtggcattc gatgcgctgg ccatcgcggg
      481 agaaagcctg atgcccaagc cgcaatcgga acgacgaaca gcactggaac gattgctggc
      541 aaaggtaaaa ccgccggtgt atctgacgcc gatgactcag gatcgcagca ttgcgctcga
      601 gtggcttgcg agatttgaag gtgccggact ggacggcgtc gttgccaagc cggccagact
      661 gccctatcag ccaggacagc gtgccatgat caaggtgaag cacgcacgca cggcagactg
      721 cgtagtggcc ggctaccgcg tatacaaaag cagtgatgac gcggtcggtt ctttattgct
      781 cgggctttat gacgatcaga ataatttgca tcatgttgga gtgacctctt cgttcacgat
      841 gccagtacgc cagcaactca tgaaggaact tgcgccctgg cgcgcaaatg cgctggcaaa
      901 tcacccgtgg cgcagctggg cacaagcgac agagggatcc actcgcatgc ccggcgcgca
      961 aagccgctgg agcgcgggca aggatctgtc gtgggaaccg ttgcgtgcgg aactcgtgtg
     1021 cgaagtgaag tacgatcatt tgcagggaga tcgcttccgg catgcagcaa cttttctgcg
     1081 ctggcggtcc gacaagcctg ccgcagattg ccgttacgat cagcttgcgg ttacgcgccc
     1141 ttacgaactg gcgaaagtct tcgagccgac gcagcaggcg cgctgatgcg ccggcgagaa
     1201 ggctgcgcgc gtatcggctc tccagcgcgt ttcttgtact gcggcggcca tggcgcatca
     1261 ccttcgccgt cgcgctcttg gcgcgcggac agttccagaa ggctgtcgag cgatcccttt
     1321 tcacggtcga tgttttcgtg ccggtcgccc agttttcgaa agcgcgcggg cattgttttc
     1381 agcgtgaaat cggccggcac ggcgtccctt aattcttccc aagcgagcgg cgcggagacg
     1441 cgcgcgtctg cagtaggccg tatggaataa gccgcagcaa tagtccggtc cttggcattc
     1501 tggttgtagt cgatgaacac ccccttgcgt tcctccttcc accatttggt ggtcgcaata
     1561 tcgggacaac ggcgctccac ttcccggcca aaagccagcg ctgcgcggcg cacctggtca
     1621 taggtccagc ggctcttgat ccgcacatag atatggagcc cgcgcttgcc cgatgttttt
     1681 ggccagccca cgaggccgaa ctcctgcaac acgtgctggc acacgtatgc cacctcacgc
     1741 acctgaggcc attcgattcc aggtacgggg tccaggtcaa cccgtaactc atccggatgt
     1801 tcgaggtcgt tcgcgcgcac cggatgcgga tgcaattcga ggcagcccag attggccatc
     1861 caaaccagtg cagcagcatg acggggcacg atttcatcgg cactgcgtcc ggacggaaat
     1921 tttatttcga caacgtcgat ccactcgggc cgggacgccg gtgcacgctt ctgatagaaa
     1981 aattcaccgt ttacgccgtt ggggtagcgc accatcatgt tcggccgtcc gccagcccca
     2041 cgcagcgcgc cagaggaaac ctccatgtag tagcgcacca catccagctt ggtgtaaccg
     2101 gcttccggga aaagcacttt ctgcggattc gaaaccggta tttccacacc gtcgatgcgt
     2161 accagttcgt gcgttgcttt tgccatgggc atgcgaccag caatgcgtat gccgcgtacg
     2221 gcgctagatc aacttgtcca gcgtaatcgg caagtcgcgg atacgcttcc cggtagcatg
     2281 aaaaacggca tttgcgactg cgcccggtac gcctgtaatg ccgatctcgc caatgccgcg
     2341 cccgccgagc ggattgaaca cctggtcatc ttctgttacg aaatcaactt caatcttcct
     2401 gacatcagca ttgacgggca catggtactc cgccagattc gcgttcacga atcggccgta
     2461 acgcgggtcg agcacgctgt gttcgaacag cgccatgccc agtccccaaa caatgccgcc
     2521 ctcaagctgg ctgcgtgccg tcttttcgtt catgaccttg ccgacactat agaccgcgct
     2581 gatgcgctgc acctgcagca tggcggtctg ttcatcgacc cgtacttcgg cgaatacagc
     2641 gccaaacgaa cggctggcaa actgcttctc ctcttcgccc ggtttagcat cgccccgggc
     2701 ttcaacgccg gagcgctgcc ggccgacaag atcgcttacc gattcgcggg cggagccctc
     2761 gcgcgctacg atccagccgt tttcgatcgt cgcatccacg gcggcgcgcc cgtgcagcgg
     2821 cgacgcggga tctgcgaccg atagtgcaat cagtttgtcg cgcaacgcaa cgcaggcatt
     2881 ttgaactgca ggtcctacgc tggcagcggt catggaacca ccggaaactg gcgcctgcgg
     2941 caacgcggtg tcgcccaata caaaacgcac gcgttccaca gccaccccga gcgattgcgc
     3001 agcgatctgc gtcatgatcg tataggttcc agtcccgagg tcctgcgtgc cggattgcac
     3061 caggacattt ccccccggca gcatgcgggc caccgctgaa gcggtcatgc gatgtccggg
     3121 ataggtggca gtggccatgc cccagccgac cagccagcgg ccgtcgcgca tggagcgtgg
     3181 ttcgcgcgtg cggcgcgccc atccgaaacg ctccgccgca agcgagtagc aggcccgcag
     3241 ttgtttgctg gaccaaggtt tgcccgtgga aggttccgct tccgcgtaat tacgcagccg
     3301 caaatccaaa ggatccatgc cgagcgcgta agcaagctcg tcgagcgcac tttcgattgc
     3361 aaaactgccg gtcgattctc ccggcgcacg ctggaaggtg ggtacgccga cattcaactt
     3421 cgccagccgt tgcgtgaccg ctccgtttgc acaagcgtaa agtgaccgcg tcggattggt
     3481 gcaggcctcg gcgaaatctt ccatgacgga agtgtgcgag ataacagcgt ggcgcaaccc
     3541 cgtaagtttg ccatcgcgcg cagcgccgag cacaagtcgc tgctcggtct gtggccgccc
     3601 accaactggt ccgaacattt gcggccgcga cagcaccagc ttgaccgggc gttgcacctg
     3661 cttcgccgcc agagcagcga gcacaacatg cgaccacgtt gatcctttgc agccaaaacc
     3721 gccgccgacg tacgggtcga tcacgcgaac cttttcgacc tcgataccca gggttttgga
     3781 caccgtctga cgtacgcccg acacatactg cgtggcgtcg tagagggtga gccgatcgtt
     3841 ttcccaatgc gcgaccgtgc cgtgcggctc aatgggattg tggtgctcca tcggggtcgt
     3901 ataaacattc tcgatcacga cgtcggccgc tcgcaggccg gcatccacat cgccccagtt
     3961 cagatccggt tccgtgccgc gtgcaagttt ctccggcttg tgcgcaaccg attttgcggc
     4021 gtcaaagcta agttgcgcgg cccgggtgac atacgtgacg tccagctttg cagccgcttc
     4081 ggtcgcctgc tcgaaggttt ccgccaccac gacggccacc ggttcgccgt tgtagtgcac
     4141 ttcgtcgtcc tgcagcaagg acatggtccg gccggcgggc gggccgtatg cggagcggcc
     4201 tttttgcggc agtgacgcaa cgttgtcgtg ggtcagcaca ccgagcacgc cgggcatgct
     4261 gcgaactgtg ccggtgtcga tgcgaacctg gccgctcggc acggtgctca gcaccagcac
     4321 accgtacgcc acatcgggcg ggaaaaactc ggccgcgtaa cgcgcgcgtc cggtgacctt
     4381 gtacggccca tcgacccggt cgatcggccg gccgatcagg ccggtgtgaa tcatgatgaa
     4441 gcctccgcga cctgcagcgc ctgcaccact gcacggcggg caagatcgat tttgaaagcg
     4501 ttatcctgca gcggttgcgc gccctccacg cttgcgcgcg ccgcggcttc gattgcgccg
     4561 ccgtccagcg ggctaccgat cagctggcgt tccgcgtttt tcacgcgcca cggcttatgg
     4621 gctacgccac cgagcacgat gcgagcatca atcactttgc cgtcctgctg gtggagcgcc
     4681 gccgcaaccg acaccagcgc gaatgcgtag ctggaccggt cgcgcacctt caggtaatgc
     4741 gagtgcgcgg agaaccgcga aggcggaagc tcaactgcaa gaatgagttc accgggcgca
     4801 agcgtggtat cgattgccgg aatgtcttcc ggaagacgat agaactggtc gatcgggatc
     4861 cggcgcgtcc cgtcgtggcc acgtacttcg acgacggcgt ccaaagcagc cagagcgacc
     4921 gccatatcag aaggatgcgt ggctacgcag tgctcgcttg cgccgaatat ggcgtgtatg
     4981 cgattgaatc cgtcgcgtgc tgcacagccg gttcccgggt cgcgtttgtt gcaggatggg
     5041 aaagccgtgt cataaaagta gtagcaacgc gtacgttgca gcagattgcc gccaacgctc
     5101 gccatgttgc gcagttgagc ggacgcaccg gccaatagcg cttgcgacag cagcggataa
     5161 tgttcgcgaa tgtagggatg atctgccacg tcgctatttc gcaccatggc gccgatgcgc
     5221 acgccgccat tgggatattg gacgatttgc gcaagtggca atcgattgat gtcgacgatg
     5281 cggaccggtc gctcgacgtc gcctttcatg aggtccagca aattggtacc gccggccaga
     5341 tattttgttt gcggagccag agcggccgcg attgcctctt caatttgttc ggctcgttca
     5401 tactcaaact cgcgcatttc gcggctcctt tagctctgag cgatgctgcg aatggcggcg
     5461 acgatgttca cataagcgcc gcagcggcac aggttcccgc tcatgcgctc gcgtatttcg
     5521 ctatctgtaa gtcgcgccgg accgttgacg cgcacatccg gcgttaccgc gctggcggcg
     5581 cccgcgttgg cctcggccag caggccgacg gccgagcaca cctggcccgg cgtgcagtat
     5641 ccgcactggt aggcatcgtg ctgcacgaaa gcccgttgca gcggatgcat cgcttccgga
     5701 acgccaagcc cttcgatggt cacgacctca tcaccatcgt gcgaaacggc cagggaaaga
     5761 cacgaattga cgcgccggcc gttgacgagc accgtgcagg cgccg
//
LOCUS       DATMZQ010000344         4219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_833278, whole genome shotgun sequence.
ACCESSION   DATMZQ010000344 DATMZQ010000000
VERSION     DATMZQ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833278"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VN496_10955"
     CDS             <1..627
                     /locus_tag="VN496_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HXF17516.1"
                     /translation="LRAAGTAHELEIAMVMYEDNATNINTVAKDVDPAARNRIIALGR
                     LDSMFKVYERLQQDAWVGILGDRILPGGEQMPADFLGQTAYFPTAPYRIALMLRRPVL
                     FMTGLYRGGNRYELHFERLFDAQAVTRTARVPAVAQALDRYAQRLEHFCRHAPYNWFN
                     FYDFWHAGPAQGDSAAGGEYLEQLAGDAQGTDRLYIDQPVSGSRSYRN"
     gene            640..1821
                     /locus_tag="VN496_10960"
     CDS             640..1821
                     /locus_tag="VN496_10960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF17517.1"
                     /translation="MPIISQRSGIDERETLFPPMLLPLFDDVFIASFDLCEEFVARTA
                     HAVLTSTGLAEACGDGATIQQALSRAGLDIGIARVPVAWLFALLASRGCLGMSRDTKG
                     DVLYRVEQSVPALEPDEILQAQQALDPRCLPSYQIASLAAAHYPAVLRGETSGEQALF
                     GADGISAWVKYFSNDNPLYAISNAIGALAAREALAAENAVILEIGGGLGSGADAFLTR
                     LRNGPGQIVQYRFTEISPLFLKRAQRTLSAKHPACRFLFAPLDIDRPFAEQGVKAGTQ
                     TLVYGVNVLHVARDLAATLSELKQALAPGGMLVMSECMRPLTGQPLHVEFVFNLLSSF
                     RDPMLVPGWRPNGGFLTPEQWRAALAANGFTDIHIFPDIASIRDFYPSFSVAAVVAQR
                     Q"
     gene            1818..2579
                     /locus_tag="VN496_10965"
     CDS             1818..2579
                     /locus_tag="VN496_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17518.1"
                     /translation="MNTALAIVARDAYFQGHFPGRPVLPAVVQLMLVLEALVREGQQP
                     AALQKITFVRLREIVLPGEQLDLVSRQMEHGRTRFDLTRKGRIVTNGELVFGNAAGAL
                     HGRKLVPAARAAALPSLDELLPHRAPMRLVTSIVGESADGLDCAACVPADCALVHEGR
                     ASILAGVEAAAQAAAAWEAVRRSREAKSAGPRIGYLVAMRDVELSAQDIPAQRPFCVG
                     VRLEAAAPPLTHYRFSVSLDGSALAQGLFATYLVE"
     gene            complement(2595..>4219)
                     /locus_tag="VN496_10970"
     CDS             complement(2595..>4219)
                     /locus_tag="VN496_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXF17519.1"
                     /translation="SRAGIKRDAARFSVLGMLLVSALLLYVYRSFRVLALTLVPVASG
                     ALAGIAAVSVVLGSVHGVTLGFGVTLIGEAVDYAIYLFTNTAPGSTPKATLQRIWPTL
                     RLGMLTSAFGFGAMLFSGFPGLTQLGVFSIAGLVVAVFVTRWVLPELLPAGYYIKTAP
                     GFGASLMRVLAYARRLRLALLASVIIAIAGLAIRGSGVWDDELSTLSPVPRPVQQLDQ
                     QMRTDLGAPDVGPLIVVTADSEQAALQTAEQVGTTLYALQAEGVLAGFDSPAFYLPSD
                     AAQRARQQAIPDARVLERNLSIAMQRLPFRSGTFAPFVEDAQAARHQPALTAQDLAGS
                     GLGLKLQGLLAQRQGRWFAMMPLRKVADTKRIETALANYDRSQVVLLDMKRELDALYH
                     GYRLRALGYALLGVVAIAVLLVVMLRSVRRTWDVLVPLAAAVLVTSAILVASGVRLNI
                     FHLVALLLVVGVGSNYTLFFERGSPGAGDPQRTATSVLFCNLSTIAGFGILAFASTPV
                     LSAIGSTVAIGAALSLAFAVILTAGSGPPARK"
BASE COUNT          852 a         1315 c         1275 g          777 t
ORIGIN      
        1 ctgcgcgccg ccggcactgc gcacgagctc gaaattgcga tggtcatgta cgaggacaac
       61 gcaacaaaca tcaatacggt agcgaaggac gttgatcccg ccgctcgaaa tcgcatcatc
      121 gcgctcgggc gcctggattc catgttcaag gtctacgagc ggttacagca ggacgcgtgg
      181 gtcggcatcc tgggcgatcg catcttaccc ggcggtgaac aaatgcccgc agattttctg
      241 ggacagacgg cttactttcc gactgcgccc tatcgcattg cgttgatgct gcgccggccg
      301 gtgttgttca tgaccgggtt gtaccgcggg ggcaaccgtt atgaactcca tttcgaaaga
      361 ttgttcgacg cgcaggccgt cactcgaacc gcgcgcgtcc ccgccgtagc gcaggcgctg
      421 gaccgctatg cgcagcggct cgaacacttc tgccggcatg ccccatacaa ctggtttaat
      481 ttctacgact tctggcacgc cggtccggcg cagggagact ctgccgccgg tggcgaatac
      541 ctggaacagc ttgccggcga tgcgcaggga acggatcgtc tgtacatcga tcagccggtg
      601 tccggttcgc gctcatacag gaattagctt cgagcgccga tgccgatcat ttcgcaacgc
      661 agcggcatcg acgagcgtga aactttgttt ccgcccatgc tgttgccgtt gttcgatgat
      721 gttttcatcg cttccttcga cttgtgtgag gaatttgtcg cgcgcaccgc ccacgcagta
      781 ctaacgtcca ccggactggc tgaggcatgc ggggatggtg cgaccatcca gcaagcgctg
      841 tcgcgcgcag gattggacat cggcatagca cgcgttcctg tggcgtggtt gttcgcgctg
      901 ctcgcctcgc gaggttgcct tggcatgagc cgcgacacaa agggcgatgt gctatatcgc
      961 gtggagcaat ctgtgcccgc acttgaaccc gatgaaattt tgcaagcgca gcaggctctg
     1021 gatccacgct gcctgccgag ctaccagata gcgtcactgg ctgccgccca ttatccagcg
     1081 gtactgcgtg gcgagaccag tggcgagcaa gcgctgttcg gggccgatgg catcagcgca
     1141 tgggtcaaat atttttccaa cgataatccg ctctacgcga tcagcaatgc gatcggcgcg
     1201 ttggcggcac gcgaagcact agctgcagag aacgccgtca tcctcgagat cggcggagga
     1261 ctcggcagcg gcgcggacgc gtttctcacc cggctgcgca acgggccagg acagatcgtg
     1321 caataccgtt tcaccgaaat ttctcctctg ttcctgaagc gcgcacagcg tacccttagc
     1381 gcaaagcatc ctgcctgtcg attcttgttc gcgccgctgg atatcgatcg acccttcgcc
     1441 gaacaaggcg tcaaggccgg tacccagacg ctggtttacg gcgtgaatgt actgcacgtc
     1501 gcccgtgatc tggcggcaac acttagtgaa ctgaagcagg cgctggcacc cggtggcatg
     1561 ctcgtgatgt cggaatgcat gagacccctc acgggacagc cgctgcacgt ggagttcgta
     1621 tttaatttat tgagttcatt ccgggatccg atgctggttc cggggtggcg accgaacggg
     1681 ggcttcctga cgccggaaca atggcgcgcg gcgctggcag caaacgggtt caccgatatt
     1741 catatctttc cggatattgc atccattcgc gacttctatc cgtcgttttc cgttgcggcc
     1801 gtggtagcgc agcgccaatg aatacagcgc tcgccatcgt cgccagggac gcttacttcc
     1861 agggacactt tccggggcgc ccggtcttgc ccgccgtggt gcagcttatg ctggtactcg
     1921 aggcgcttgt gcgcgaaggt cagcagccgg ctgcactaca gaagatcacg ttcgtgcgcc
     1981 tgcgcgaaat cgtactgccc ggcgaacaac tcgatctcgt ttcgcgccag atggagcacg
     2041 ggcgcacgcg ttttgatctc acacgcaaag gccgcatcgt caccaacggg gaactggtgt
     2101 ttggaaacgc ggccggcgct ctgcatggca ggaagctcgt tccggcagcg cgagccgccg
     2161 cattgccatc cctggatgag ttgttgcccc accgggcgcc aatgcgattg gtcacatcga
     2221 ttgttggcga aagcgccgat ggactggact gcgcggcgtg cgtcccggcc gactgcgcgc
     2281 tggttcacga aggccgtgca tctatcctgg ccggtgttga agcggcggcc caggctgctg
     2341 cagcttggga ggcggtgcgc cgctcgcggg aagcgaagag cgctggaccg cgcatagggt
     2401 acctcgtcgc aatgcgcgat gtggaattgt cggcgcaaga cattcccgca caacggccgt
     2461 tttgcgttgg cgtgcgccta gaagcggcag cgccgccgct cacgcattat cgattctcgg
     2521 tgtcgctcga tgggtcggca cttgcacaag gcctgttcgc tacttatctg gtcgagtaat
     2581 cgcaagcatc cagtttactt tcgagccggc ggccccgaac cagcagtcag gataacggcg
     2641 aatgcaaggc tgagcgcagc accaatcgcc accgtagatc caatggcgct caacacaggt
     2701 gtgctggcaa aggccagaat cccgaaacca gcgatagttg acaggttgca gaacagtacc
     2761 gacgtggcag tccgctgagg atcgccagcg cccgggctgc ctcgctcgaa gaacagcgtg
     2821 tagttcgagc cgacgccgac cacgagcaga agcgccacca gatggaaaat attcagccgc
     2881 acaccgctcg caaccaggat cgcgctggtg accagcactg cagcggcaag cggcacaagc
     2941 acatcccagg tccgccgcac cgagcgcagc atcactacga gcagcaccgc gatggccacc
     3001 acgccgagca gcgcatagcc cagcgcgcgc agtcgataac cgtgatacaa agcatcgagt
     3061 tcccgcttca tgtccagcaa tacgacttgc gatcgatcat aattggccaa ggcggtttca
     3121 atccgcttcg tgtcggccac tttgcgcagg ggcatcatcg caaaccagcg gccctggcgt
     3181 tgcgcaagca aaccttgcag tttcaggcca agcccgctgc cggccagatc ctgtgccgtc
     3241 agcgccggct gatgtcttgc cgcttgcgca tcttcgacga acggcgcgaa ggttccggaa
     3301 cggaacggca ggcgctgcat ggcgatcgat aaattacgct ccaacacgcg ggcgtcgggt
     3361 atagcctgct ggcgggcacg ctgggccgca tcgctcggca ggtaaaacgc tggcgagtca
     3421 aatcccgcga gaacgccctc ggcttgcagg gcataaagtg tcgtaccgac ctgctctgcc
     3481 gtctgcagcg ccgcctgctc ggagtccgcc gtcaccacga tcagcggacc gacatcgggc
     3541 gcgcccaaat ccgtacgcat ctgctgatca agctgttgca ccggccgcgg caccggactc
     3601 agggtggata gttcgtcgtc ccacacgccg gatcctcgga tcgccaggcc ggcgattgca
     3661 atgatgaccg acgcgagcaa agcaagtcgc agacggcgcg catatgccag cacgcgcatc
     3721 aatgacgcac cgaacccggg tgcggttttg atgtaataac ccgcaggcag caattcgggc
     3781 aatacccagc gcgtcacgaa taccgcaaca acgagtcccg cgattgagaa cacgccaagt
     3841 tgcgtaaggc ccgggaaccc ggagaacagc attgcgccaa agccaaaggc cgacgtcagc
     3901 atgcccagcc gaagagtcgg ccagatgcgc tgcaaagtcg ccttcggcgt gctgcccggg
     3961 gcggtattgg tgaacaggta tatggcatag tccaccgctt ctccgatcag cgtaacgccg
     4021 aaccccagcg tgacgccatg caccgatccc agaacaacgc tgaccgcggc aatgccagcc
     4081 agtgctccac ttgccactgg caccaaggta agggccagca cgcgaaagct ccgatagaca
     4141 tacagcagga gagcgctgac cagcagcatg ccgagcacag aaaaacgcgc ggcatcgcgc
     4201 ttgatgccgg cacggctct
//
LOCUS       DATMZQ010000345         9537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3043646, whole genome shotgun sequence.
ACCESSION   DATMZQ010000345 DATMZQ010000000
VERSION     DATMZQ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043646"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1119)
                     /locus_tag="VN496_10975"
     CDS             complement(151..1119)
                     /locus_tag="VN496_10975"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXF17520.1"
                     /translation="MKTTFLDFEQPIAELEARIEELRYVQDDSAVDISEEISRLQKKS
                     STLTRDIYGKLSAWQISQVARHPQRPYSLDYFQNLFADFEELQGDRLFAEDPSIIGGL
                     ARFGSQSVMVIGHQKGRDTKDKLYRNFGMPKPEGYRKALRLMKLAEKFGIPVMTFVDT
                     PGAYPGVGAEERGQSEAIGRNLNAMAELRVPIVVTIIGEGGSGGALAIAVGDVTLMLQ
                     YAIYSVISPEGCASILWKSADKAPDAAETLGITANRLKTLGLVDRVVAEPPGGAHRDP
                     TTIMQTLRKSLMDALSEAQEKPIDALLETRFERLMAYGKFREAAVR"
     gene            complement(1168..1443)
                     /locus_tag="VN496_10980"
     CDS             complement(1168..1443)
                     /locus_tag="VN496_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF17521.1"
                     /translation="MKWLLMLLPFFPLPLLAGEEAVRLKEAPGKNLVEANCSMCHSLD
                     YIPMNSPFLDRKGWEASVGKMIKVMGAPVAEADVQNIVDYLVSQYGK"
     gene            complement(1452..2681)
                     /locus_tag="VN496_10985"
     CDS             complement(1452..2681)
                     /locus_tag="VN496_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXF17522.1"
                     /translation="MRSRRQFLKASAGSLLLAGANRWAPSADAANEIEPASLPAGTLE
                     TAQFGSLTGKLPLIKKSFRPPNFETPVNYFNEVFTPNNAFFVRYHLAGIPELDAAQWK
                     LTIAGDSVEKPFEIGYEKLTTDFDVVEVAAVCMCSGNRRGFSQPHVPGVQWGHGAIGN
                     AKWRGVRLRDLLTKAGLKKDAVEIVFDGADSAVLEKTPDFVKSLPAWKAMDENTLVAF
                     AMNGERLPHWNGFPARMVVPGWTATYWMKHLTSITAVPQPFKGFWMNPAYRIPKGKFP
                     VIDRFVSQETETNTPITEMVVNSLITSPNAGTKLRQGQSTTVMGVAWDGGYGIAMVEV
                     SEDNGKSWQAAQLGPDQGRYSWRQWSHRYQPSRAGRQTVLARASNRAGTTQTFELIFN
                     PAGYHNNVVQRVDMDVI"
     gene            complement(2706..4388)
                     /gene="ggt"
                     /locus_tag="VN496_10990"
     CDS             complement(2706..4388)
                     /gene="ggt"
                     /locus_tag="VN496_10990"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXF17523.1"
                     /translation="MIKINKMFGCLLTVALMVTGASRAVAAEQCGIASAHELATNAGC
                     EILRSGGNAFDAAVAVAAALAVVEPFASGIGGGGFFLLHRASDNVDVFIDARETAPAR
                     ATPDFFVDNTGHAKQKAALEGPTAAGIPGEPAALDWVARRYGKLSLARTLAPAIRLAK
                     QGFAADARYAAASRMQESLLQSNGAAARIFLDHGKAAAAGFLVIQPQLADTLHMIGAE
                     GGTSFYRGDFARRLVTQVQTAGGLWELSDLENYRVIERAPVKFTYRGASITTSPLPSS
                     GGLVLAQSLFILESMPMSRVSESERAHYIVEAMRRGYQDRARFMGDPGFFAVPVKKLA
                     SREYALARGASIDPQRATPSSVLAQDSAAVEEGADTSHFSIIDAAGNRVAATVSVNGP
                     FGSGFVAGDTGVILNNHMDDFVLVPGVPNLYKLVGSKANAIQPGKRPLSSMSPTFVED
                     DRGVLVLGTPGGSRIISMVLESILDYVDRAEFDLKRLVATPRFHHQYLPDRIEIEPGS
                     FSDDWIEALKDKGHVVEPGKRKWGNMQAVFRDRKTGRIETASDPRGQSGVLF"
     gene            complement(4449..7586)
                     /locus_tag="VN496_10995"
     CDS             complement(4449..7586)
                     /locus_tag="VN496_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF17524.1"
                     /translation="MKRFNLSEWALEHQSLVLFFMLALFAAGLFSYFRLGQAEDPEFT
                     FKIMVVRALWPGASPVQMEQQVTERLEKKLQETPYLDYVRSYTKAGEATIFVALKDAT
                     PPREVGGIWYQVRKKIEDIRYTLPAGTVGPFFNDEFGDVFGNIYAFTGDGYTYSELKD
                     YVEEVRKEILRVPAVAKAEIIGDQDQKIYVETSHRKLATLGIDPLSIFNVLREQNAMT
                     PAGAIDTRSDRIFLRISGSFDSVESIREIGIQANGRLFRLGDIAHVYRGYVDPPSQKV
                     RYMGQDAIALAVSMAKGSDILQLGRDLKDAFARINRDLPVGIEVHQYSNQPEVVKRSV
                     DEFMRTLLEAVIIVLAVSFFSLGFRTGLVVALSIPLVLAVTFLMMKLYGIDLQRISLG
                     ALIISLGLLVDDAIISVEMMATKMEQGMERTKAAGFAYTSTAFPMLTGTLITAAAFTP
                     VGFAKSSAGEYTFSIFAVVTIALLVSWIVAVLFTPYLGYKLLPERLHGAPMFSAFQRF
                     IHAPLHRWRPALIPAPAAKPGGDVYDSRFYRRFRALVDWCVNYRKTVILITVAVFALS
                     IFGFRFVQNQFFPSSNRPELIVDLWLPQGASFQATEVQVKRLEQKLKGDENVSNFVAY
                     VGNGSPRFYLPLDQQLVHPNFAQFVIVTRNNNAREVLYERINRLLESDFTMLRGRVNR
                     LENGPPVGYPVQFRVIGNDIATVRKIAFEVASVVRAHPNSQNVHLDWNEQVKDIRLEI
                     DQNKARVIGVSSQGLSTVLNSILTGYSITQYREKDELIEVLARAETQERLDPGKLAEI
                     NVPTQSGKWIPLAQIANIEYGFEEGIIWRRNRQATVTVRADIRGNVQAPVISTQINPK
                     LDTIRAHLPEYYRIEMAGAIEESAKGENSIVAVMPIMVITVLTLLMIQLQSFQKTFMV
                     LLTAPLGLIGVAFILLTWNVPFGFVANLGVIALFGMIMRNSVILVDQIEQDIKAGHPQ
                     WNAIVDATVRRFRPIVLTALAAVLAMIPLSRSNFWGPMAVAIMGGLVSATVLTLLFVP
                     ALYAAWFRVRRPTAA"
     gene            complement(7583..8689)
                     /locus_tag="VN496_11000"
     CDS             complement(7583..8689)
                     /locus_tag="VN496_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF17525.1"
                     /translation="MSLLWRPHAPAILHIVSFIAVVCGCSEKQQAAPEPRLVSAIRVS
                     SGSGTNDVAYSGEIRPRYESNLSFRVPGKIVARNVEVGSIVAKGNPLARLDPEDQNLN
                     TRSAESQQAAARSEYEQSKAELERYTDLYQKQFISKAEFDRHQNAFNVAKARLEQSQA
                     QLAVSKNQTGYTELSADHAGVVTAILVEVGQVVAAGQTVMKIARTDDREVSINVPENR
                     LAELSTARQIDISLWSDPSRIYKGKLREVSPSADPVTRTYAAKISVLNADGAMKLGMT
                     ANVYLRGLQRTSSMLLPATALFQENGHAAVWVVDKSGKVSLVRVEISEYVDDRVGVIS
                     GLSPGDLIVRAGVHKLYPGEKVRVHEEPVTEAGK"
     gene            complement(8747..9445)
                     /locus_tag="VN496_11005"
     CDS             complement(8747..9445)
                     /locus_tag="VN496_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXF17526.1"
                     /translation="MRALIAAMLVVIAIAGCTRGELVSFRGVTEGEVIRLAAPVAGNL
                     SSLDVRLGATVTQGARLFSQADAGEISAHADAAKRLDETEHIWGKRSGSEADEIARLK
                     AEVAQTEWKLLLKSASAPVSGVVTETLYSKGDWVPAGAPVIAILPTDRIKVNFEVPLA
                     VAAHLQNGRAVTLLCAQCSTPVRATITYVSPFATAESASSGASRELHYRVEARPLPDQ
                     AALLKPGQSITVNL"
BASE COUNT         2033 a         2832 c         2588 g         2084 t
ORIGIN      
        1 cgatggcgac atgcgttccg cttacggcgt gcgcgtccaa cacaccgtcg acgattgccg
       61 caagttcctc attcatgatt attgggagtc aaagtacgat aaccgcgctt gtgcgggcta
      121 tcggcaagaa taatgagcga gcgcgacgtt tcagcgcacc gccgcttccc tgaactttcc
      181 atacgccatc aggcgctcga agcgcgtttc cagcagtgca tcgatcggct tttcctgagc
      241 ttcggaaagc gcgtccatga gcgatttgcg caaagtctgc atgatggtcg tcggatcccg
      301 atgagcgcca ccgggaggtt cagcgaccac gcggtcgacc agaccaaggg tttttaagcg
      361 gttggccgtg atgccgaggg tttccgcagc atccggggcc ttatccgcac ttttccacaa
      421 gatcgacgca catccctcgg gagaaatcac tgagtagatc gcatattgca gcatgagcgt
      481 tacgtcacca actgcgatag cgagcgcgcc ccccgacccg ccttctccga taatcgtgac
      541 gacaattggc acacgcagtt ccgccattgc gttcagattg cgtccgatcg cctcggattg
      601 tccgcgttcc tccgctccaa cgcccggata agcacccggc gtgtcgacga aggtcatcac
      661 cgggatgccg aatttctccg cgagcttcat tagccgcagg gctttgcgat acccctcggg
      721 cttcggcatg ccgaaattac ggtacaactt atccttggtg tcgcgccctt tttgatggcc
      781 gattaccatt actgattggc tgccaaatcg tgccaatccg ccgatgatgg agggatcttc
      841 ggcaaacaaa cggtcgcctt gcagttcttc gaagtccgcg aacaaattct ggaaatagtc
      901 gagggaatag ggccgctgtg gatgacgcgc gacttgcgaa atctgccacg cagaaagctt
      961 tccgtaaatg tcccgcgtca gcgtgctgct ctttttttgc agccgcgaaa tctcctccga
     1021 gatatcaact gcagaatcat cctgcacata gcgcagttct tctatccgcg cctccagctc
     1081 cgcgatcggc tgctcgaaat cgagaaaagt tgttttcatg gcactgaatt gtatcgacag
     1141 gtcgacgcgc gcatttgctg caatcgctta tttaccgtat tgggacacca gatagtcgac
     1201 gatgttttgc acgtctgctt ccgccacagg ggctcccatc accttgatca tcttgccgac
     1261 tgatgcttcc catcccttgc gatccaggaa aggagagttc attggaatat agtcaaggct
     1321 atggcacatc gagcaattgg cctcgacgag attcttcccc ggcgcttcct tgaggcggac
     1381 cgcttcttct cccgccagaa gcgggagcgg gaaaaacggg agcagcatta gcagccactt
     1441 catttaacct cctagatcac atccatatcg acgcgctgaa ccacattgtt gtggtatccg
     1501 gcgggattga aaatcagttc gaatgtctgg gtggtaccgg cgcggttgct cgcccgcgcc
     1561 agcacggttt gccggccggc acgagagggc tggtagcgat gggaccattg gcgccaggaa
     1621 tagcggccct gatcaggccc caattgcgcc gcctgccaag acttcccgtt atcctccgac
     1681 acctcgacca tcgcgatgcc atacccacca tcccaggcga cgcccatcac ggtagtggac
     1741 tgtccctgcc gaagttttgt gccggcgttc ggactggtaa taagcgagtt gactaccatc
     1801 tcggtgattg gtgtgttggt ctcggtttcc tggctcacga atcggtcgat caccgggaat
     1861 ttgcctttgg ggatgcggta ggccgggttc atccagaagc ctttgaacgg ctggggtaca
     1921 gcagtgatgg aggtcaggtg cttcatccag tacgttgcgg tccagccagg cacgaccata
     1981 cgcgccggaa agccgttcca atgcggcagc ctttcaccat tcattgcaaa cgcaaccagc
     2041 gtattttcat ccatcgcctt ccacgccggc aggcttttca cgaaatcggg agtcttctcc
     2101 aacacggcgc tgtccgcgcc atcaaatacg atttcaaccg catccttctt caatcccgcc
     2161 ttggtcagca aatcgcgcaa gcgtacgccg cgccatttcg cattgccaat ggcgccatgg
     2221 ccccattgca ccccggggac atgcggttgc gaaaatccgc gtcgatttcc ggaacacatg
     2281 cacaccgcgg caacttcgac gacatcgaaa tcagtcgtca gtttttcata accgatctcg
     2341 aagggctttt ctaccgagtc gccagcaatg gtcaatttcc attgtgcagc atcaagttct
     2401 ggaatgccgg ccagatgata gcgaacaaaa aacgcattgt tgggcgtaaa aacttcgttg
     2461 aagtaattga caggtgtttc aaagttcggc gggcggaacg atttcttgat cagaggtaat
     2521 ttgccggtca ggctgccgaa ttgggcggtt tcgagtgtgc cggccggcaa tgacgccggt
     2581 tcgatctcat ttgctgcatc cgcggatggc gcccagcgat ttgcaccggc cagcagcagg
     2641 ctgcccgcag atgccttcag aaactgccgt ctgcttctca tcgctttttc cttgctccga
     2701 acggcctaga acaacacgcc gctctgcccg cgagggtcac tggcggtttc gattcgtcct
     2761 gtttttctgt cccggaatac ggcttgcatg ttaccccact tgcgcttgcc gggctccacg
     2821 acatgccctt tgtccttgag cgcttcgatc cagtcgtccg aaaaactgcc cggttcaatt
     2881 tcgatccggt caggcagata ttggtgatgg aagcgcggcg tagccaccag acgcttgaga
     2941 tcgaattccg ctcgatccac ataatcaaga atgctttcca aaaccatcga aatgatgcgc
     3001 gaaccgccgg gtgtgccgag caccagtaca cctcgatcgt cctccacaaa tgtcggggac
     3061 atggacgata acgggcgctt gcccggctga atggcgttgg ctttgctgcc caccaacttg
     3121 tacaggttcg gtacgccagg cacgagcaca aaatcgtcca tatggttatt cagaatcacg
     3181 cccgtatctc cagcgacgaa gccagagccg aatggtccgt tcaccgacac ggttgctgca
     3241 acgcggttcc cggccgcatc aatgatggag aaatgcgaag tgtccgcgcc ttcctcgact
     3301 gccgcagagt cttgcgccaa cacgctgctc ggcgttgccc gctgcggatc gatggaagcg
     3361 ccgcgcgcta atgcgtattc acgcgaagcc agttttttga ctggcacggc aaagaaacca
     3421 ggatcgccca tgaagcgcgc gcggtcctgg taaccgcgcc gcatcgcctc gacgatatag
     3481 tgcgcgcgtt cgctttcgga aacgcgggac atcggcatcg attccagaat gaacagactt
     3541 tgcgccaata ccagtccacc tgatgacggc aacggtgagg tcgtgatgct ggcgccgcga
     3601 tatgtgaatt tcacaggcgc tcgctcgatc acgcggtagt tttcgagatc ggataattcc
     3661 cacaaaccgc cggccgtctg tacctgcgtc accagtctgc gcgcgaaatc gcctcgatag
     3721 aagcttgttc cgccttcggc gccgatcatg tgcagcgtgt ctgcgagttg cggctggata
     3781 acgaggaagc cggccgcagc cgccttgcca tgatccagaa agatgcgcgc ggctgcgcca
     3841 ttactctgca agagcgattc ctgcatccgg cttgcggcag catagcgggc gtccgcggca
     3901 aacccctgtt tcgcgagccg gatggccggc gccagtgtgc gtgcaaggga cagtttgccg
     3961 tagcgcctgg cgacccagtc gagtgccgcc ggttcaccgg gaatgccggc cgcggtcggg
     4021 ccctcgagcg ccgctttttg tttggcatgt ccggtattgt cgacaaagaa atcaggcgtt
     4081 gcgcgagcag gcgccgtttc acgagcgtcg atgaatacat caacgttatc gcttgcccgg
     4141 tgcaacagga aaaatccacc gccaccgatg ccggatgcga atggctccac caccgccagc
     4201 gcggctgcga ccgcgacggc agcatcgaag gcattgcccc cagaacgcag tatctcgcag
     4261 ccggcattgg tcgccagttc atgcgcggac gcgatcccgc actgctccgc ggcaacggcg
     4321 cgcgatgcgc ccgtgaccat cagtgcgacg gtcagcaaac atccaaacat cttattgatt
     4381 ttgatcatgt gcactctaca agaattgaaa tggcatttca ggctgcttgc cgcaaaagca
     4441 gctacgcatc aagctgcggt gggccttctg acacgaaacc atgctgcgta aagggctggc
     4501 acaaacaaca gtgtcagcac ggtcgcggac accaggccac ccatgatggc gacggccatg
     4561 gggccccaga aattgctgcg tgacagggga atcatcgcca acacggccgc aagcgcggtg
     4621 agcacgatcg gtctgaatcg acgcaccgtg gcgtccacaa tggcattcca ttggggatgg
     4681 cccgccttga tatcctgctc gatctgatca acgagtatca ccgagttacg catgatcatg
     4741 ccgaacaagg ctatgacgcc aagattggcg acaaagccga atggcacgtt ccatgtcagc
     4801 agaatgaatg ccacgccgat cagcccgagc ggcgcagtca gcagcaccat gaatgtcttc
     4861 tggaaacttt gcagctggat catcagcagc gtcaggaccg tgatcaccat gatcggcatg
     4921 acggcaacaa tcgaattctc tcctttggca ctttcctcga tcgcgcccgc catctcgata
     4981 cggtaatact ctggcaagtg tgcgcgaatc gtgtccagct tcggattgat ctgcgtcgat
     5041 atcaccggcg cttgcacatt gccacgaatg tccgcgcgca ctgtcaccgt tgcctgcctg
     5101 ttacggcgcc agataatgcc ttcttcaaag ccatattcga tgttcgcaat ctgcgcgagc
     5161 ggaatccact ttccggattg ggtcggcacg tttatttcgg caagtttgcc gggatcgagc
     5221 cgctcctgtg tttctgcgcg cgcgagcacc tcaatcaatt cgtctttctc gcggtactgg
     5281 gtgatgctgt agcctgtgag tatcgaattg agcaccgtgg acagtccttg cgaacttaca
     5341 ccaatgacgc gagccttgtt ctggtcgatc tcaagacgga tgtctttcac ttgctcgttc
     5401 cagtccagat gcacattctg cgaattggga tgggcgcgta cgacggatgc gacttcgaaa
     5461 gcgatcttgc gtacggttgc gatgtcattg ccaatcacgc ggaactgcac gggataacca
     5521 accggtggac cgttttccag ccggttcacg cgcccgcgca gcatcgtaaa atcactttcc
     5581 agcaggcgat tgatccgttc gtacagcacc tcacgcgcat tattgttcct ggtcacgatc
     5641 acgaactgcg cgaagttcgg atgaacgagt tgctggtcga gtggaagata aaagcggggg
     5701 ctgccgttgc caacatacgc gacaaagtta cttacgtttt cgtcgccttt gagcttttgc
     5761 tccaaccgct tcacctgaac ctcggtcgct tgaaaagaag cgccttgcgg caaccagaga
     5821 tcgacgatca attcgggccg gttcgatgac ggaaaaaact ggttctgcac gaagcgaaag
     5881 ccgaatatcg acaatgcaaa taccgcgacc gtgatgagga tcacggtctt gcggtaattc
     5941 acgcaccagt ccaccagcgc ccggaagcgc cgataaaacc ggctgtcgta cacatccccg
     6001 ccgggcttcg ctgcgggtgc cggtataagc gccggacgcc accggtgcag aggagcatga
     6061 atgaagcgct ggaatgcgga aaacattggc gcaccgtgca ggcgttccgg caacagcttg
     6121 tagccaagat agggcgtaaa caataccgcc acaatccagg atacgagcaa cgcgatggtc
     6181 acgaccgcga aaattgaaaa cgtatattcg ccggccgacg atttggcaaa tccgaccggg
     6241 gtaaatgcgg ccgcggttat taatgtaccg gtaagcatcg ggaaggcggt tgaggtatag
     6301 gcaaagcctg cggctttcgt ccgctccatg ccctgctcca ttttggtcgc catcatttcc
     6361 accgaaatga tagcgtcgtc caccagcaag cccagcgaga taatcagtgc acccagcgaa
     6421 atgcgctgca ggtcgatgcc gtagagcttc atcatcagaa aggtcacggc gagaaccagg
     6481 ggaatagaca aggcgacgac gaggccggta cgaaagccca gactgaagaa actcaccgcc
     6541 agaacgataa tgaccgcttc aagcagcgtt ctcatgaatt cgtcgaccga tcgtttcaca
     6601 acctccggct ggttggaata ctggtgcact tcgatcccga ccggcagatc gcggttgatg
     6661 cgcgcaaatg cgtccttgag atcccgtccg agttgcagga tgtcactgcc cttggccatc
     6721 gatacggcca gcgcaatcgc gtcttgcccc atgtagcgta ctttttgcga aggcggatcc
     6781 acatagccgc gatacacgtg cgcgatatct cccagcctga acagccgccc gttcgcctgg
     6841 atgccgattt cacggatact ttctaccgaa tcgaaactcc ctgaaatacg caggaatatt
     6901 cgatccgaac gagtatcgat tgcacctgcc ggcgtcattg cattttgctc gcgcagcaca
     6961 ttgaagattg aaagcggatc aatgcccagc gtggcgagct ttcgatgaga cgtctcgacg
     7021 tagattttct gatcctgatc gccgatgatt tccgcttttg ccacagccgg cacgcgcagg
     7081 atctccttgc ggacctcttc gacgtaatcc ttcagttccg aatacgtgta accgtcgccg
     7141 gtaaatgcgt atatgttgcc aaacacatcg ccgaattcat cgttgaagaa cgggccgaca
     7201 gtgccagcag gcaacgtata gcggatatcc tcgatttttt ttcggacctg ataccagatt
     7261 ccaccgactt ccctcggggg cgtagcatcc ttcagcgcaa cgaaaatcgt cgcttcacca
     7321 gccttggtat aggagcgtac gtaatccaga tagggcgtct cctgcagttt tttttccagg
     7381 cgctcggtca cttgctgctc catctggacc ggcgaagcgc ccggccacag cgcccggact
     7441 accatgatct tgaacgtgaa ctcagggtcc tccgcctgac caagcctgaa ataggaaaac
     7501 aatccggcag caaacaatgc cagcataaaa aaaagcacca acgactggtg ctccaatgcc
     7561 cactctgaca agttgaatcg cttcactttc cggcctctgt taccggctcc tcatgtacgc
     7621 gcactttttc tcctggatag agtttgtgaa caccggcgcg tacgatcaaa tctcccgggc
     7681 tcagtccgga tatgacgcct acccggtcat cgacatattc cgaaatttcg acacgcacga
     7741 gggagacttt ccctgatttg tcgactaccc agaccgccgc atgcccgttt tcctgaaaca
     7801 gcgccgtggc gggcagcagc atcgaggagg tgcgctgcaa gccccgcaaa tacacattcg
     7861 cggtcatgcc gagtttcatg gcgccgtcgg cattgagcac gctaatcttg gccgcgtagg
     7921 tgcgcgtcac cgggtctgcc gacggactaa cttcccgcag ctttcctttg tagattcttg
     7981 acggatccga ccagagcgag atatcgattt gcctggcggt actcaattcg gccaggcggt
     8041 tctcgggaac gttgatactg acttcccgat cgtccgtgcg cgcaatcttc attactgtct
     8101 ggccggcagc aacgacttgg cccacctcga ccaatatcgc ggtcaccaca ccagcgtgat
     8161 cggccgatag ttcggtgtaa ccggtttgat ttttcgacac ggcgagctgg gcctgtgact
     8221 gttccagtct cgccttggcc acgttgaagg cgttctgatg ccgatcgaat tccgccttgc
     8281 tgataaattg cttctggtac agatccgtat agcgttcgag ctcggctttg ctttgttcgt
     8341 attcggaccg ggccgcggct tgctgggatt ctgcgctacg tgtgttgaga ttttgatctt
     8401 cggggtcaag ccgcgcgagc ggatttccct tggctacgat gctccctacc tcgacattac
     8461 gggccacgat cttgccgggc acccgaaagc tcagattgct ctcgtaacgg ggccggatct
     8521 ctccggaata cgcaacatca ttggtgccgc tccccgaact gactcgtatg gcactgacaa
     8581 ggcggggttc gggggctgct tgctgctttt cgctgcaccc gcaaacgacc gcaatgaacg
     8641 atacgatgtg cagaatggca ggtgcatgcg ggcgccaaag tagactcatt ttggcgggcc
     8701 gctcacgcca tgcaacttgt gtcaccagcc gtaaacggga cggctgtcac aagttaacgg
     8761 tgatcgactg accgggcttt aataatgctg cctgatcagg cagcgggcga gcttcaaccc
     8821 ggtagtgcaa ttcccgagat gcgcccgatg aagcagattc tgcggtggca aagggagaaa
     8881 cataagtaat cgttgcgcgc actggagtgc tgcactgtgc gcagagtaag gtcacagcgc
     8941 ggccgttttg taaatgcgcg gccaccgcca atggcacttc gaaattgacc tttatccgat
     9001 cggtcggaag gatggcgatc accggcgcgc cggcgggaac ccaatcgccc ttggaataaa
     9061 gtgtttcggt taccactccg cttaccggcg cactggctga cttcagcagc aatttccatt
     9121 cggtctgtgc aacttcggct ttaagcctgg caatctcgtc cgcttcggat ccgctgcgtt
     9181 ttccccaaat gtgctcagtc tcgtccaagc gcttggctgc gtcggcatgc gcagagattt
     9241 ccccagcgtc cgcctgcgaa aataatctgg cgccctgggt caccgttgcg cccagcctga
     9301 catcaaggga agacaaatta cctgccacgg gcgctgccag ccggattact tctccctccg
     9361 taacgccgcg gaaagacacc agctctccac gcgtgcatcc tgcaatagca attaccacca
     9421 gcatcgcggc aatcagggct cgcatcgcac ttatccgtgc agtgtcggtc acttcgcgtc
     9481 ttccttgagc aggccgttga tgtcggccag atcggcttca gccagtttgc ctacggc
//
LOCUS       DATMZQ010000346         6805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3388753, whole genome shotgun sequence.
ACCESSION   DATMZQ010000346 DATMZQ010000000
VERSION     DATMZQ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388753"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..414
                     /locus_tag="VN496_11010"
     CDS             223..414
                     /locus_tag="VN496_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17527.1"
                     /translation="MTFRELSARILEPASMLLMILGIVALCQPWIEVFHRYGVTITLI
                     GLVGFSVFSKIKPLPGEEA"
     gene            414..1556
                     /locus_tag="VN496_11015"
     CDS             414..1556
                     /locus_tag="VN496_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXF17528.1"
                     /translation="MRLRMGMVGGGLDAMAGQLHRRAAALDSRVELVAGALSSTPERS
                     LAAGKAWGLARSYGTWQEMLQCEAKLPAGERIEFVTVATPNHLHARISTAFAESGFHV
                     ACDKPIAISLEEARHLCRVVDRSGIVFLLTHNYTGYPMVKQARHMIATGELGKLVKVL
                     VDYPQGWLLQPLENQGHKGAAWRTDPSMSGAAGCLADIGSHAQHMMHTVTGLEIEALC
                     AELGHLHGRKVDDDISLLMRLRGGARGVLTASQVMAGEEGGPNFRIYGTHGALEWKQL
                     EPESLLYRRPGAPVHILRRGEPYLCDTARRASRLPPGHPEGYYEAFGNLYRNFADAII
                     ARREGVRIEEDFPTAVDGARTMAFIETALESNAAGGQWMRVKDHAH"
     gene            1621..2730
                     /locus_tag="VN496_11020"
     CDS             1621..2730
                     /locus_tag="VN496_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17529.1"
                     /translation="MADIVLRDVEKYFGDNYIIRKLNLEIRHREFVVLLGPSGCGKTT
                     TLRAIAGLEEIDSGDILIDGKPVQALHAADRDIAFVFQLYALYPHLTSYENIAFPLRA
                     MNESRETVDKRVREAAKALKIEHLLSQRPGALSGGDLQRVAIGRALVRRPKALLMDEP
                     IGALDAKLREEMRTELKRLHMENGATTVYVTHDQVEAMSMADKIAIMNDGVLQQVGTP
                     TEVYSHPANLFVAQFIGSPIMNVVKAAARLGDDSTRVTFGENDAEQDFPKQLHHFLAE
                     KSQAGEELTIGVRPEGVLVSRSQSAGYLPVEAHIIEPLGPFDIVDLKFGTQMLRARTA
                     SGYVAKAGDTVWAKLDETQTHFFSTRSGESLHIRL"
     gene            2736..3818
                     /locus_tag="VN496_11025"
     CDS             2736..3818
                     /locus_tag="VN496_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17530.1"
                     /translation="MAHVTLKGISKKFRSHTALSGLELDIASGEFFVLLGPTGAGKTT
                     TLRLIAGLDHPDAGSIAIDEQNVMGWSAAERDVALVFQYYSLYPHYTVRQNLEFPLKS
                     KVRRMSQAQIDERVAKAAKTLRIGQLLERKTDRLSGGEMQRVSIGRAIVREPKVFLMD
                     EPLSNLDAKLREILRAELKDLQMKLGATFLYVTHDQVEAMSMGDKIGVLNQGRLIQVG
                     TPQQIYNRPRDVFVATFVGSPAMNLLPATVSNGRAVVSPGELEVPINHAGNGQAGKLS
                     GQVTLGIRSEDVTVGMNEAVEARVHGVENQGVEKIVTLRAGGCLIKGTVPARMELRID
                     SAIRFSFKQEKMHFFATESGNNLYYG"
     gene            3829..5103
                     /locus_tag="VN496_11030"
     CDS             3829..5103
                     /locus_tag="VN496_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF17531.1"
                     /translation="MAKILFKNVNVLDCSGVPPFAGQVLVEGNRIKAVAPQGSTIPSE
                     GARVVDGGGATLMPGLIESHSHLSFLDTPDLEGLGFVPPEEHILRTVRNAKKMLDQGF
                     TACNSAAAAKPRLDVVLRNAINAGDFPGPRTLAASPEMTVTSGLGDVRLMHMHRETFA
                     VICDGAEEFRKMSRLMVREGVDTLKINPSGDEFIPHARAHQTVMTEAEIAAVCDVGHA
                     HGKRMAAHARSAQSVKLCLKHGVAVIYHATLVDEEACDALEAAKDWCFVAPTLGINYT
                     TLNEAAKWGITPQVAESMGMRIELDAGIKNMKELKKRGVRILPGGDYGFAWNPIGTNA
                     RDIEHFVNLLGFSPMDAILSATKLGGEIMMAGKELGQVKPGYLADMILVDGNPVANVK
                     LLQDANKLLMIMKDGQFHKEPVVAAQAQRQIA"
     gene            5241..6353
                     /locus_tag="VN496_11035"
     CDS             5241..6353
                     /locus_tag="VN496_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17532.1"
                     /translation="MNGFPGELAAERFRALFACLAALFLLCIAGCGGGHSSSSTATRT
                     WQLGFSPVPPRLTTQAVLGGIDLWSQRAEIAAIHEELPWTDLLGGVLTPDDILDRDKV
                     QLVNYMRGKGLKLYFMGELTDGLSRGEEAPQLRALGRSIKEPAVQQAYRDYVLAVARK
                     LRPEIIGLVAETNLIRAAATDPLLYPAVVQAANAAASDLRAAGNTATLLYSVQVETAW
                     GKLNPAAPNYVGVEQDFADFPFAQTLGLSSYPYFGFAQPEDIPANYYSRLLNGRSMPT
                     MVVEGGWTSAAAGSIQSSPQIQARYIARHAQLLDMVGARGLIQLLFADIDLATFPQPY
                     PPNLPLFVNIGLTDSNFAAKPALGAWDELHARRLIP"
     gene            6378..6608
                     /locus_tag="VN496_11040"
     CDS             6378..6608
                     /locus_tag="VN496_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF17533.1"
                     /translation="MKQPLVLLPGLLCDAALWEPQTSDLADVAEFFVADFTSQHSIGE
                     MAASALRAAPCEKFALAGLSMGGYVAQEIMRQ"
     gene            6618..>6805
                     /locus_tag="VN496_11045"
     CDS             6618..>6805
                     /locus_tag="VN496_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17534.1"
                     /translation="MTKLALLDTRSRAELPEETERRRELMQLAQSGRNFTPVTNRMLP
                     FLLHASRIDEAPLVEVIR"
BASE COUNT         1465 a         1924 c         2086 g         1330 t
ORIGIN      
        1 aaggaaatga agggccgtga ataacatgaa tatcgtaaat gtcgtgaatg tcgtaacagt
       61 cctcatgcga aatggccttg ggagactagt tagagggctt ttgaaattcg cggcatttac
      121 ggtattcacg atattcacgg catttatggg cgtagcccgg ttatggcgaa gcctgaacac
      181 cacgcgtgat ccccttgggg acccggtagt tacggcattc acatgacttt ccgcgaactc
      241 agcgcccgca tcctcgagcc ggcgtccatg cttctgatga ttctcggcat cgttgcgctg
      301 tgccagccat ggatagaggt gtttcaccgc tatggcgtga cgatcacatt gattggtctg
      361 gtcgggtttt cggtattctc gaaaatcaag ccgttacccg gggaagaagc gtgatgcgct
      421 tgaggatggg catggtcggg ggaggcctgg acgcgatggc ggggcaatta catcgtcgcg
      481 ctgccgcgct ggacagtcgc gtcgaactgg tggcgggtgc attgtcctcc acgccggagc
      541 gctcgctcgc cgcagggaaa gcatgggggc tggcgcgaag ctacggcact tggcaggaaa
      601 tgctgcaatg tgaagcaaag ctgccggccg gggagcgcat cgaatttgtt acagtcgcta
      661 cgccgaacca cttgcacgca cgcatttcca ccgcgtttgc tgaaagcggg tttcatgttg
      721 cctgtgacaa accgatcgcc atttctctcg aagaagcccg gcatttgtgc cgcgtagtgg
      781 accgtagcgg catcgtgttt ctgttgacgc ataactacac cggctacccg atggtgaagc
      841 aggcgcgcca tatgatcgcc accggagaat tgggcaagct cgtcaaggtg ctggtggact
      901 atccgcaagg atggctgttg cagccgctgg agaatcaggg acacaagggt gcggcatggc
      961 gcacggatcc ttcgatgtca ggcgcggcag gatgcctggc cgatatcggc tcgcatgctc
     1021 agcacatgat gcataccgtg acggggctgg aaattgaagc gttgtgcgcg gaactcggac
     1081 acctgcacgg ccgcaaggtg gacgacgaca tcagcctgct gatgcgcctg cgcggcggtg
     1141 cgcgcggagt actgacagcg tctcaagtca tggcgggcga ggaaggcggt ccgaatttcc
     1201 gcatttatgg cacgcacggc gcattggagt ggaaacagct ggaacccgaa tcgttgcttt
     1261 atcgaaggcc gggagcgccg gtgcacatcc tgcgccgcgg cgagccctac ctgtgtgata
     1321 ccgccaggcg cgccagccgc ctgccgccgg gacatcccga aggctattac gaggcgttcg
     1381 gcaatctgta ccggaatttt gcggatgcga tcatcgcgcg ccgggaagga gtccgcattg
     1441 aagaggattt tccgacggcg gtcgatggcg cacgtaccat ggcgttcatt gaaaccgcgc
     1501 tggaaagcaa cgccgccggc ggccaatgga tgcgcgtgaa agaccacgcg cactagcggg
     1561 taacgctgta tatattcacg acattcacga cattcacgat attcacggca ttcacgaaca
     1621 atggccgata tagttttacg ggacgtcgaa aaatatttcg gcgacaacta catcatcaga
     1681 aaactcaatc tggagatccg gcaccgcgag ttcgtggtgc tgctgggacc ttccggttgc
     1741 ggcaagacga ccacgctacg tgccattgcg ggcctggaag aaatcgacag cggcgacata
     1801 ctgatcgatg gcaaacccgt gcaggcactg catgccgccg accgcgacat tgctttcgta
     1861 tttcagctct atgccctcta tccgcacctg acgtcgtacg agaacatcgc ctttcccctg
     1921 cgcgccatga acgaatcgag ggaaaccgtg gacaagcgcg tacgtgaggc ggccaaggcg
     1981 cttaagatcg aacacctgct cagccagcgg ccgggtgcgc tgtccggcgg cgacttgcaa
     2041 cgggtagcga ttggccgggc gctggtgcgg cggcccaagg cgctgctgat ggacgagccg
     2101 atcggcgcgc tggatgccaa attgcgcgaa gaaatgcgca cggaactcaa gcgcctgcac
     2161 atggaaaatg gcgcgaccac ggtttacgtg acgcacgacc aggtcgaggc catgtcgatg
     2221 gcggacaaga tcgccatcat gaacgacggc gtgctgcagc aggttgggac cccgaccgag
     2281 gtttacagcc atccggccaa tctgttcgtt gcacaattca tcggcagccc gatcatgaac
     2341 gtggtcaagg cggccgcgcg cctcggagac gacagtacgc gggtgacatt cggcgaaaac
     2401 gatgccgagc aggatttccc gaaacaactt caccattttc tggcagagaa gagccaggcc
     2461 ggtgaagaac tgaccatcgg tgtgcgtccc gaaggcgtgc tggtatcgcg cagccaaagt
     2521 gccggctatc tgccggtgga ggcgcacatc atcgaaccgc tcggcccgtt cgatatcgtt
     2581 gacctcaagt tcggcacgca gatgctgcgc gcccgcacgg cctccggcta cgttgccaag
     2641 gccggtgaca ccgtgtgggc gaagctggat gaaacacaaa cccatttctt cagtacccgc
     2701 agcggcgaat cgctgcatat ccgcttgtag gcgacatggc gcacgtcact ctcaagggca
     2761 tcagcaagaa atttcgtagc cacaccgcgc tgagcggact ggaactggac atcgccagcg
     2821 gcgaattttt cgtcctgctc ggacccaccg gggcgggaaa gaccacgacg ctgcgcctta
     2881 tcgccggatt ggatcatccc gatgccggca gcattgccat cgatgagcag aacgtgatgg
     2941 gatggagcgc ggcagagcgc gacgtggcgc tggtgttcca gtattactcg ctgtatccgc
     3001 actacaccgt gcgccagaac ctcgagtttc ccctgaaatc caaggtgcgg cggatgtctc
     3061 aagcgcagat cgacgagcgc gtggccaaag cggcgaagac cctgcgcatc ggccagttgc
     3121 tggaacgcaa gaccgaccgg ctgtcgggcg gtgaaatgca gcgagtctcc attggccgcg
     3181 ccattgtgcg cgaacccaag gtgttcctga tggatgaacc attgtccaac ctggatgcga
     3241 agctgcgcga aatcttgcgc gcggaactga aggatctgca gatgaaactc ggtgcaactt
     3301 ttctttacgt cacgcacgac caggtggaag caatgtcgat gggcgacaag atcggtgtgc
     3361 tcaatcaggg ccgccttatc caggtcggta cgccgcagca gatctacaat cggccaaggg
     3421 atgtattcgt tgcgacgttt gtcggatcgc cagccatgaa tttgctgcct gcgacggtca
     3481 gcaacgggcg cgcggtggtg tcgccgggtg agctggaagt gccgataaac catgccggta
     3541 acggacaggc gggaaagctg tccggccaag tcacactggg catccgttcg gaagatgtca
     3601 ccgtagggat gaacgaagca gttgaagccc gtgtccatgg ggtagagaat cagggggtgg
     3661 agaaaatcgt aaccctgcga gcaggaggtt gtctgatcaa aggcaccgta cccgcgcgca
     3721 tggaattgcg tatcgattcg gccataagat tttcgttcaa gcaggaaaaa atgcattttt
     3781 tcgctaccga aagcggcaac aatctgtatt acggttgagg aggaagtcat ggctaagatt
     3841 ctgttcaaga atgtcaatgt actggactgc agcggcgtgc cgccattcgc gggccaggta
     3901 ttggtagaag gcaatcgcat caaagcggtc gcgccgcaag gctcgaccat cccaagcgag
     3961 ggggcgcgcg tggtcgacgg cggcggtgcc acgttgatgc ccggtttgat cgaatcgcac
     4021 tcccacttat cttttttgga tacgccggat ctcgagggac tcggtttcgt tccgcccgaa
     4081 gaacatatct tgcgcacggt ccggaacgcc aaaaaaatgc tcgaccaagg attcactgct
     4141 tgcaacagcg cggcagcggc caaacctaga ctcgatgtcg tattgcgcaa tgcgatcaac
     4201 gccggggact tcccgggacc gcgcacgctg gcggccagtc cggaaatgac tgttacatca
     4261 ggcctcggcg acgtgcgcct gatgcacatg caccgtgaaa cttttgctgt catctgcgac
     4321 ggcgcggaag aattccgcaa aatgtcgcga ctgatggttc gcgaaggcgt agatacactg
     4381 aaaatcaatc catctggcga cgagttcata ccgcatgcgc gggcgcacca aaccgtgatg
     4441 acagaagccg agattgccgc ggtttgcgac gtagggcacg cgcacggcaa gcgcatggcc
     4501 gcccatgcgc gcagtgcgca gtcggtcaag ctatgcctga agcatggcgt agcagtgatc
     4561 tatcacgcga ctctggtcga cgaagaagcc tgtgatgcac tggaagcggc aaaagactgg
     4621 tgctttgttg cgccgacatt gggcatcaac tacaccacgc tgaacgaggc ggccaaatgg
     4681 ggcatcaccc cgcaggttgc cgaaagcatg ggtatgcgca tcgaactcga tgccggtatt
     4741 aaaaacatga aggaacttaa aaagcgcggc gtccgcatcc tgccaggcgg cgattacgga
     4801 ttcgcctgga atccgatcgg caccaacgcg cgcgacatcg agcacttcgt caatctgctg
     4861 ggtttttctc ccatggatgc gattttgtcg gccacgaagc tcggtggtga aatcatgatg
     4921 gcgggcaagg aactcggtca ggtgaaaccg ggctatcttg ccgacatgat cctggtggac
     4981 ggcaatccgg tggccaacgt gaagctgctg caggatgcca acaagctgct catgatcatg
     5041 aaggacggtc aattccataa agagccggtc gtcgccgcgc aggcgcaacg gcagatagcg
     5101 tagtcaagaa aaagaaaaaa caataaaccg tcaaaggatc gcgcccgcat gcgggcgctt
     5161 ttttttgcgg ctgaacaagc gcgccggcag gttagccggt cgacgggcat aatctcgatt
     5221 tcaagaatac atgcggacat atgaacggct tccctggtga gttggcggcg gaaagattcc
     5281 gtgcgctgtt tgcttgtttg gccgcactgt ttctgttgtg tatcgctgga tgcggtggcg
     5341 ggcattcgtc gtcctccact gcaacgcgca cctggcaatt gggcttttcc ccggttccac
     5401 cgcgactaac aacccaggca gtgctgggcg gtatcgatct ctggagccag cgcgccgaga
     5461 tcgcggccat tcacgaggaa ttgccgtgga cggatctgct gggtggagtt ttaacgcccg
     5521 atgacatcct tgaccgcgac aaggtccagc tggtgaatta catgcgcggc aaaggcctca
     5581 agctctattt catgggcgag ttgacggacg gcctatcgcg cggcgaggag gcaccccagt
     5641 tgcgcgcgct cgggcgcagc atcaaggagc ctgcggtgca gcaggcatat cgcgactacg
     5701 tgctggcggt ggcgcgcaag cttcgtccgg aaatcattgg cctcgtcgca gagaccaatc
     5761 tgatccgcgc tgccgccacg gatcccttgc tctatccggc ggtcgtgcaa gccgccaacg
     5821 cggctgcgtc agatctgcgc gcagccggaa acacggcaac cctgctgtac agcgtgcagg
     5881 tagagacagc ctggggcaaa ttgaatcccg ctgctccaaa ttacgtgggc gtcgaacagg
     5941 atttcgccga ttttccattt gcgcaaacac tcggattgtc gtcgtatcct tacttcggat
     6001 tcgcgcagcc cgaagacatc ccggccaact actacagccg actgcttaac ggacgttcga
     6061 tgccgacgat ggtggtcgag ggtggctgga ccagtgctgc cgcaggcagc atccagtcgt
     6121 ctccgcagat acaagcccgt tacatcgcgc gccatgccca actgctcgat atggtcggcg
     6181 cacgcggcct gattcaactg ttgtttgccg atatcgacct tgcaacgttt ccgcaaccgt
     6241 atccgccgaa tctgccgctg ttcgtcaata ttggattgac cgatagcaac ttcgctgcca
     6301 agcccgcgct gggggcgtgg gatgagttgc acgcgcggcg cctgataccc tagattcccc
     6361 ggcaaaggca tcactccatg aagcagcctc tggtccttct tcccggtttg ctgtgcgacg
     6421 ccgcgttgtg ggaaccgcaa acatcagatc ttgccgatgt cgcagaattt ttcgttgcgg
     6481 acttcaccag ccagcacagc atcggtgaga tggctgcatc ggctctgcgc gcggctccgt
     6541 gcgaaaaatt cgcgctcgca ggcctgtcga tgggtgggta tgttgcccag gaaataatgc
     6601 gtcagtagcc gcagcgggtg accaagctgg cgttgcttga tacccgttcg cgcgccgaat
     6661 tgcccgagga aaccgaacgc cggcgcgaac tcatgcaact cgcgcagagc ggccgcaact
     6721 ttacgccggt gaccaaccgc atgctgcctt tcctgctgca tgcctcgcgc atcgacgaag
     6781 ctccgctcgt cgaagtcatt cgcga
//
LOCUS       DATMZQ010000347        14424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2010078, whole genome shotgun sequence.
ACCESSION   DATMZQ010000347 DATMZQ010000000
VERSION     DATMZQ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010078"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VN496_11050"
     CDS             <1..605
                     /locus_tag="VN496_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HXF17535.1"
                     /translation="LRPNGAAGLLVSHIGAFRDYEFSKAWVWEHQALTRARFVAGDTQ
                     IGAGFERIRTEVLCRPRDLASLAAEIQSMRDKMRDAHPNASGLFDLKHDRGGIVDVEF
                     VVQYLVLGHAHRHPELTGNIGNLALLKLAASLGLLPEELAAGGHAAYREFRQLQHAMR
                     LQGERYARVDAAAVAHHALCVQRVWQFVFASVVPTPGQSE"
     gene            complement(593..1879)
                     /locus_tag="VN496_11055"
     CDS             complement(593..1879)
                     /locus_tag="VN496_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXF17536.1"
                     /translation="MVELSKRAMPSRRGLISRLGAAAIDENDSDEVRLRKTLLLFASG
                     LMNMAAILWLMIYWSMGLKLPTTIPLGFQIASALILAIYLRTRNFDFFRMAQLGLFLF
                     FPFVIQWAIGSFVSSSGIALLALLAPVGAMVCYGPRESIPWFIAYVVLTVLSGVFDFF
                     LGGGDYGIPTKTVAVFFVMNFTIISTIVYLLLRHFVQERDRFEGKLAEQHLLVRTERE
                     KSEKLLHSILPAHIAERLKHQPGNIADGFADVCVMFADLVNFTRLAEEFTPIQVVSFL
                     DEVFTRFDQLTERHGLDKIKTIGDAYMVVGGLQESQRNYADHVADMALDIIKLTRSDP
                     QMSRLGVQLHIGIATGPAIAGVIGATRFIYDVWGDTVNLASRLTAEADPDSILVDKTT
                     FRRLGYRYACEEPREIEIKGKGTTATYRLTGKLYSD"
     gene            2060..2980
                     /locus_tag="VN496_11060"
     CDS             2060..2980
                     /locus_tag="VN496_11060"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HXF17537.1"
                     /translation="MSMADRDGVIWYDGKLVPWRDANTHVLTHSLHYGLAVFEGLRAY
                     KTKSGTAIFRLKEHTERMFNSAHIYMMKIPYDRETLMEAQKEVVRANKLDSCYVRPIA
                     FYGSEKMGVSPKGARVHVAIAAWPWGAYLGADGIEKGIRVKTSSYARHHVNVSMCRAK
                     YSGTYANSILANMEATEHGYDEGLLLDVDGFVAEGAGENLFMVKNNRIFEPELNSALI
                     GITRDSVIVLAGDLGYTVDAKRITRDDLYIADEVFFTGTAAEVTPVREVDGRTVGAGK
                     RGPLTEKLQGMFFDVVNGRTEKYRHWLHYV"
     gene            2987..3193
                     /locus_tag="VN496_11065"
     CDS             2987..3193
                     /locus_tag="VN496_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HXF17538.1"
                     /translation="MEPAAANSNRLKVVEITASDLPLHCPLPSQALWNSHPRVFLPIG
                     GDGEALCPYCGTRYVVRGATKAGH"
     gene            3208..4221
                     /gene="waaF"
                     /locus_tag="VN496_11070"
     CDS             3208..4221
                     /gene="waaF"
                     /locus_tag="VN496_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HXF17539.1"
                     /translation="MSRVLIVAPSWVGDALLSQPLFTLLKGSEPEAIIDVLAPGWALP
                     VFRRMPEVNDIIESPFAHGELALRKRLRLGRSLRDRHYARAYVLPNSFKSALVPLFAR
                     IPQRIGFIGEARRGLLTDARRLDEHVLPRMVEQFAGLALPPGEHITAELPQPRLQVQP
                     AESRRLFAELGLDAPARLVCLCPGAEYGPAKRWPPEYFGELAAALAADGIAVWVIGSA
                     KEREIGETIRAGSGGTAVNLCGRTTLDQAVVLLARADMVVSNDSGLMHVAAALDRPMI
                     ALYGSSSPRFTPPLSGKARVVKIDVPCSPCFKRVCPLGHFDCMMKLTPGRVLAEIQSH
                     LHD"
     gene            4214..4639
                     /locus_tag="VN496_11075"
     CDS             4214..4639
                     /locus_tag="VN496_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXF17540.1"
                     /translation="MISRTYATPQDAELAFYRAFELADLVEMMAVWAEEEEIICVHPG
                     GGRHVGIVEVRESWRQIFSQGPRLRFALSGSHVVAGRTLSVHSVYENVSVAGNPRPAN
                     PVLSTNIYLLTNRGWRMMVHHASALAAAPAPQETPSVLH"
     gene            4639..5613
                     /locus_tag="VN496_11080"
     CDS             4639..5613
                     /locus_tag="VN496_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXF17541.1"
                     /translation="MPRYQAPWWLPGGHLQTLYPYFFLKPQAPPYRRERWETPDGDFI
                     DLDWVIQEQNQSSPLVALFHGLEGGSGSHYATALMHAVMRNQWRGVVVHFRGCSGEPN
                     RLPRAYHSGDADEIAWILQRLRRLDAAFPLFAAGVSLGGNALLKWLGREQTAAREMID
                     AAAAVSAPLDLMTAGDRLGAGLNRLYGLHFLQTLKPKSLAKLERFPGLYDGQVVSKAR
                     TLREFDNVVTAPLHGYRDTDDYWQRASSKPDLRGIAVPTLIVHARNDPFLPGPHLPTA
                     SDVSQSVTLDFPEHGGHTGFASGPFPGNLDWLPQRLLAFFSTRISAHR"
     gene            5695..7071
                     /locus_tag="VN496_11085"
     CDS             5695..7071
                     /locus_tag="VN496_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXF17542.1"
                     /translation="MSLPASEIFKAYDIRGVVGKTLTPQVTEMVGRAVGSQARELGQS
                     RLCIGRDGRLSGPDLSEALARGVQSAGVDVIDIGRVTTPMLYFATHHLQTGSGVMVTG
                     SHNPPDYNGLKMMIAGETLSGEAIQGLRRRIESGKFAAGTGGYSQTQVAEAYLQRITG
                     DIKLKRKMRIAVDCGNGVPGAFAPELFRRLGCEVEELFCDVDGTFPNHHPDPSQPENL
                     EDLIAALRKGSAEIGLAFDGDGDRLGVVTKQGKVIFPDRQLMLFAADVLKRNPGAEIL
                     FDVKCTRNLAPWIRQHGGKPTMWKTGHSLIKAKMKETGALLAGEMSGHIFFKERWYGF
                     DDGAYAGARLLEFLSSVQDIKSTFDSLPDSINTPELQIRLAEGENYALIDQLQKNANF
                     SSAREVIKIDGLRVEYADGFGLARSSNTTPVVVLRFEADNDNALKRIQEEFRGVLQKA
                     APEAKLPF"
     gene            complement(7068..7610)
                     /locus_tag="VN496_11090"
     CDS             complement(7068..7610)
                     /locus_tag="VN496_11090"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF17543.1"
                     /translation="MNKLTKWTAAMLLVASALPAFGDAQDLTIFHTITINAPADEVWA
                     VIGDFGGIQRWAPGTESSRLVLHDRNETGAIRLLRRRGDGTQVTEKLLDYDPYNRRMA
                     YTYVDGVVRAADYYSEVAVKDAGSGRSIVEWRGRFKRLAYWTDQPPAGQDDKAALDFL
                     NGAYKSGLDNLKKLLEQQQR"
     gene            complement(7642..10260)
                     /gene="alaS"
                     /locus_tag="VN496_11095"
     CDS             complement(7642..10260)
                     /gene="alaS"
                     /locus_tag="VN496_11095"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041492808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXF17544.1"
                     /translation="MKSSEIRTRFLQYFARQGHTIVASSPLVPANDPTLLFTNAGMVQ
                     FKDVFLGHDKRPYVRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIRFAWELLTREYGLDSKRLWVTVYETDDEAYDIWANEIKVPRERIARIGDKPGGQ
                     KFQSDNFWQMGDTGPCGPCTEIFYDHGPGIPGGPPGSVDADGDRYIEIWNLVFMQFNR
                     DDSGTMHPLPKPSVDTGMGLERIAAVLQGVHSNYEIDLFQDLIRAAAKVTGAKDLNSS
                     SLRVIADHIRACSFLITDGVIPGNEGRGYVLRRIIRRAIRHGYKLGKTKPFFHKLVAE
                     LSRVMGDAYPELTTAQGRVTDVLKQEEERFAETLENGMKVLENALHREDRMLDGETVF
                     QLYDTFGFPVDLTADIARERGVMIDHAGFEEAMQRQRERARAASKFTVHSSVDYSGRA
                     TEFHGYETLRLEAHVIALYRDGSQVQSVSAGEDAVVVLDRTPFYAESGGQVGDQGEIT
                     SGNGGFHVADTRKIQAEVFGHHGKVTAGRLSVGDAVTAQVDATNRALAAYNHSATHLM
                     HAALRKVLGEHVTQKGSLVDANRTRFDFSHHAPLTAQQISEVEDLVNREIRENHPVET
                     RRMKYDDAIKAGAMALFGEKYGDEVRVLSMGDFSTELCGGTHVRRTGDIGFFKIVSES
                     GVASGIRRVEAVTAKGAVAWVHDQETRLNQAAQVLKVQPQELTQKLGQILDNVKSLEK
                     ELSRLKSKLANTQGDDLADQAVAVGASRVLAARLDGADARSLREAMDKLKDKLKSAAI
                     VLGAIEGDRVTLIAGVTSDLTAKVKAGELVNFVAQQVGGKGGGRPDMAQAGGTEPAKL
                     RMALDSVPDWVRQRIA"
     gene            complement(10349..10795)
                     /gene="recX"
                     /locus_tag="VN496_11100"
     CDS             complement(10349..10795)
                     /gene="recX"
                     /locus_tag="VN496_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HXF17545.1"
                     /translation="MPAPLPGSLRARALKALARREHSRDELEAKLRPFAESPDDLGPL
                     LDDLEKHGWLSEARFVEHVTTVRRRRFGAARILRELRDKGVSEDALAALQPQLKNSEI
                     EAARTVWRKKFGKLPATLAERAKQSRFLASRGFSAEIVHQVLCNDE"
     gene            complement(10804..11823)
                     /gene="recA"
                     /locus_tag="VN496_11105"
     CDS             complement(10804..11823)
                     /gene="recA"
                     /locus_tag="VN496_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXF17546.1"
                     /translation="MDENRSKALAAALSQIEKQFGKGSIMRLGEKDIAKDVEVVSTGS
                     LGLDLALGIGGLPRGRIVEIYGPESSGKTTLTLSVIAQMQKLGGTAAFIDAEHALDPQ
                     YAQKLGCNVSELLISQPDNGEQALEIADMLVRSGSVDVVVVDSVAALTPKAEIEGEMG
                     EPQMGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRIDIRRIGSIKKGEEVVGNETRAKVVKNKVAPPFRQAEFDILYGEGISLEGELIE
                     LGVLYKMIEKSGAWYSYGKDRIGQGKDNTREFLKEHPDVAKEIENKIRSHVAEKPLSP
                     VLEPE"
     gene            complement(12079..12573)
                     /locus_tag="VN496_11110"
     CDS             complement(12079..12573)
                     /locus_tag="VN496_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HXF17547.1"
                     /translation="MDEEIYELAERVGETLQAYKFMMSTAESCTGGWIGQAVTMVPGS
                     SGWFDRGFITYTNDAKHDMLGVRHATLSQFGAVSEQTVLEMVAGALARSRAQVAVAVS
                     GIAGPDGGSISKPVGTVLLAWGQKGVSTRAQAMHFPGDRDAVRRQSVIAALDGILALL
                     HPQS"
     gene            complement(12592..13095)
                     /locus_tag="VN496_11115"
     CDS             complement(12592..13095)
                     /locus_tag="VN496_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HXF17548.1"
                     /translation="MTTFAESPARPTVRFVYSHPAHFVSFGFGVGLIPVAPGTFGTLL
                     AFPLYTWLVYRLGEPVFALLMLLLFALGVWACQVTGKALRVHDHSGMVWDETVAFLIV
                     LFFTPASLVWQAFAFLAFRLFDILKPAPIRYVDRTVRNGLGVMFDDLLAAFYTLLCLA
                     GCKLLFG"
     gene            complement(13079..14062)
                     /gene="thiL"
                     /locus_tag="VN496_11120"
     CDS             complement(13079..14062)
                     /gene="thiL"
                     /locus_tag="VN496_11120"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXF17549.1"
                     /translation="MTRPAASRPALSEFDVISRYFTRAPRHTELGIGDDAALLRVTAG
                     SELAASTDMLVEGVHFFADVDPQALGHKALAVNLSDLAAMGAMPRWAMLALAIPEADT
                     QWLEAFSRGFFALADKHGVDLIGGDTTRGPRNICVQIMGEVASGQALRRDGAKIGDDI
                     WVSGELGAAAAAVAHRRGEMNLPPAVFEQCRVRLDWPAPRVALGLELSALANSAIDVS
                     DGLVGDLAHICERSGVGAQIHSAAVPCAAVLQPLRHQPSVTRAMLTGGDDYELCFTAS
                     PSRAAEITALSATLEIELTRIGSIVDGNKVSVLDASGGPLSVEDNGFDHFR"
     gene            complement(14096..>14424)
                     /locus_tag="VN496_11125"
     CDS             complement(14096..>14424)
                     /locus_tag="VN496_11125"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXF17550.1"
                     /translation="AGCFAIVLEAIPDRVAQVITQKLTIPTIGIGAGPHCDGQNLVLH
                     DMVGLFDRFTPKFVKRYANCWDVIGKALAEFQDDVHKGQFPLAEHSFTMKEDEYQAFL
                     KKLDHS"
BASE COUNT         2932 a         4345 c         4129 g         3018 t
ORIGIN      
        1 gcctgcggcc caacggcgcc gccggattgc tggtcagcca catcggcgca ttccgcgatt
       61 acgaatttag caaggcctgg gtttgggaac atcaggcgct tacgcgcgcg cgctttgtcg
      121 cgggtgatac tcaaatcggc gcaggctttg agcgtattcg tacggaagtt ctgtgccggc
      181 cgcgtgacct cgcgtcgctt gctgccgaaa tccagtccat gcgcgacaag atgcgcgacg
      241 cacacccgaa tgccagcgga ctgttcgatc tcaagcatga tcgcggcggc atcgtcgatg
      301 tggaattcgt ggtgcagtac ctggtgctcg gccatgcgca ccggcatccg gaactcacgg
      361 gcaatatagg aaatcttgcc ctgttgaagc tcgcggcgag tctgggtttg ctcccggaag
      421 aactcgccgc aggcgggcat gctgcgtacc gggaattccg gcagctccag catgcaatgc
      481 ggctgcaggg cgaacgctat gcgcgtgtgg acgcagccgc cgttgcgcat catgcgctgt
      541 gtgtgcagcg ggtttggcag ttcgtctttg ctagcgtcgt gccgacgccg ggtcaatcgg
      601 aatagagttt gccggtcagc cggtaggtgg ccgtggtgcc tttgcccttg atctcgattt
      661 ccctgggctc ttcgcaggca tagcggtatc cgagacggcg gaatgtggtt ttgtccacca
      721 ggatggagtc gggatcggct tcagccgtca gccggctagc cagattcacg gtatcacccc
      781 agacgtcata gatgaatcgc gtggcaccaa tcacgccggc gatcgcagga ccggttgcga
      841 taccgatatg aagttgcacc ccgagacggc tcatctgcgg atcggatcgg gtcaacttga
      901 tgatgtccag cgccatgtcg gcgacgtgat ccgcataatt gcgctggctc tcctgcagtc
      961 cgccgaccac catgtacgca tcgccgatgg tcttgatttt gtccaaccca tgacgttcgg
     1021 ttaactggtc aaagcgtgta aatacttcgt ccaaaaacga aacgacctga atcggcgtga
     1081 attcctcggc cagcctggtg aaattcacca ggtccgcgaa catgacgcaa acgtcggcga
     1141 atccgtcagc aatgttgcct ggctgatgtt tcaaacgttc cgcgatatgg gccggcagga
     1201 tactgtgcag cagtttttcg gatttctcgc gctcggtgcg caccagcagg tgctgttcgg
     1261 cgagcttgcc ttcgaagcga tcgcgctcct gcacaaagtg tctcagcaac aaataaacga
     1321 tagtcgagat gatggtgaaa ttcatcacga agaaaaccgc aaccgttttt gtcggaatgc
     1381 cgtaatctcc gccgcccagg aagaaatcaa acacgccgga caataccgtc aataccacat
     1441 acgcgatgaa ccacggtatt gactcgcgcg ggccgtagca gaccatcgct cccacggggg
     1501 cgagcaaggc aagcagcgcg atcccggacg aacttacgaa actgccgatc gcccactgga
     1561 tgacaaaggg aaaaaacagg aacaggccaa gctgcgccat gcggaaaaaa tcgaaattcc
     1621 gtgtgcgcag ataaatcgcc aggatcaggg cggatgcaat ctggaacccc aatggaatag
     1681 tggttggaag cttcaatccc atcgaccagt aaatcatcag ccacaggatc gccgccatgt
     1741 tcatgaggcc gctggcaaac agcaacaacg ttttgcgcag cctcacttca tcgctgtcgt
     1801 tttcatcgat cgccgctgcg ccgagtcgcg aaatcagccc tcgacgggac ggcatggcgc
     1861 gtttggacaa ttcaaccatg ttcagtgatc aggggactcg ggctttgaaa acgttgttct
     1921 tcggcagccg caatgggttg gcgggtcagc gcgaagagta atgcatgccg gctcgttccg
     1981 gcaaggcggc ctgccataag cgcgcataaa ttgcgccggg ctgcgctaga attacgactt
     2041 tttccaggag cgaagcaaca tgtcgatggc ggaccgggac ggtgttatct ggtatgacgg
     2101 aaaattggtc ccgtggcgcg atgccaacac acatgtactc acccattcgt tgcactatgg
     2161 actagctgta ttcgaggggc tgcgcgccta caaaaccaaa agcggaaccg cgatcttccg
     2221 gctgaaggag cacaccgaac gcatgttcaa ctcggcgcac atctacatga tgaagatccc
     2281 ttacgatcgc gagacgctca tggaggcgca gaaggaagtc gtgcgcgcca acaaactcga
     2341 ttcctgttac gtgaggccga ttgcgttcta tggttccgag aagatgggcg tgtcgccaaa
     2401 aggcgccaga gtgcatgttg ccattgctgc ctggccatgg ggcgcttatt tgggcgcgga
     2461 tgggatcgag aaaggcatcc gcgtgaagac ttcgtcgtac gcccgccatc atgtcaacgt
     2521 ttcgatgtgc cgcgcgaagt attcgggcac gtacgctaat tcaatcctgg cgaacatgga
     2581 agcaaccgag catggctacg atgaaggcct tttgctcgat gtggacggtt tcgtcgccga
     2641 aggagccgga gagaacctat tcatggtgaa gaacaaccgc atcttcgagc ccgaactcaa
     2701 ttcggcactg attggcatta cgcgcgactc tgtcattgtg ctggctggcg acctaggcta
     2761 cacggtcgat gccaagcgca ttacccgcga tgatctatat attgccgacg aggtattctt
     2821 cacgggaacg gcggccgaag tcacgccagt ccgcgaagtg gacggtcgca ccgtcggcgc
     2881 cggaaagcgc ggcccgctca ccgagaagtt gcaaggtatg tttttcgatg tcgtcaacgg
     2941 ccgcactgaa aagtatcgcc actggctgca ctacgtctga agcgccatgg aacccgcagc
     3001 cgccaacagc aaccgactta aagtagtgga aatcacggcg tccgaccttc cgctgcactg
     3061 tccgctgccg tcgcaggcct tgtggaactc gcatccgcgc gtattcctgc ccatcggggg
     3121 cgatggtgaa gccttgtgtc cctattgtgg cacgcgctac gtcgtgcggg gtgcgacaaa
     3181 agcgggccac taaacttgcg gcatagtatg agcagggtcc tgatcgttgc accttcctgg
     3241 gtcggcgatg cgctgttgtc gcagccgctg ttcacactcc tcaaaggctc agagcctgaa
     3301 gcgataattg atgtgttggc ccccggctgg gcgttgccgg tttttcggcg catgccggaa
     3361 gtgaatgaca tcatcgaaag tccgtttgcg cacggcgagc tcgcgttgcg aaaacggctg
     3421 cgactcggac gaagcttgcg cgacaggcat tacgcgcgtg catatgttct gcccaattcg
     3481 ttcaagtctg ccttggtgcc gttgtttgca cgcattccgc agcgcattgg cttcatcggt
     3541 gaagcgcgcc gtggcctgtt gactgatgcg cgccgcctgg acgaacacgt gctgccccgc
     3601 atggtggaac agtttgcggg attggcgttg ccgcctggcg aacatatcac ggcagaactg
     3661 ccgcagccgc gattgcaggt ccagccagca gagtcccgtc gtctgttcgc cgagcttggg
     3721 ctggatgcgc cggcccggtt ggtttgtcta tgtccgggtg ccgaatacgg ccctgccaag
     3781 cgctggccgc ccgaatattt cggcgaactc gcggcggctc tcgccgcgga tggcattgcc
     3841 gtttgggtta tcgggtccgc caaggaacgc gaaatcggtg aaacaatccg cgcgggcagt
     3901 ggcggcacgg cagtgaattt gtgtggacgc accacgctcg atcaggcggt cgtgcttcta
     3961 gctcgcgccg atatggttgt ctccaacgat tccggcctca tgcatgtggc tgcggcgctt
     4021 gaccggccga tgatcgcgct gtacggctcc agttcaccgc gattcacgcc cccgttgtcc
     4081 gggaaagcgc gcgtcgtgaa aatcgatgtt ccgtgcagcc cctgcttcaa gcgcgtctgt
     4141 ccgctgggac atttcgattg tatgatgaag ctcacgccgg gccgcgtgct cgctgagatc
     4201 cagagtcatc tgcatgatta gcagaactta cgccacgccg caggatgcgg agctggcctt
     4261 ttaccgcgca ttcgagcttg ccgacctggt ggaaatgatg gcggtatggg cagaggaaga
     4321 agagatcatc tgcgtccatc ccggaggagg gcgtcacgtc ggaatcgttg aagtgcgcga
     4381 atcctggcgc cagatttttt cccagggacc tcgtctgcgt ttcgcactgt ccggcagtca
     4441 tgtagttgcc ggacgcacat tgtcggtaca cagcgtgtac gaaaatgtaa gcgtcgcagg
     4501 caatccgcgt ccggcgaatc ctgtgttatc cacgaacatc tacctgctca ctaatcgcgg
     4561 ctggcgcatg atggtccatc atgcttcagc gcttgccgcc gcacccgcgc cgcaggaaac
     4621 cccgtccgta ctgcactgat gccgcgctac caagcgccct ggtggttgcc gggtggtcat
     4681 ttacagaccc tctacccgta tttttttctg aaaccccagg caccgcctta tcgccgcgaa
     4741 cgctgggaaa ccccggatgg cgacttcatc gacctcgatt gggtgatcca ggagcagaac
     4801 cagtccagtc ctctggtcgc gctgtttcat ggtcttgaag gtggatcggg cagccactac
     4861 gcgactgcct tgatgcacgc cgtaatgcgt aaccagtggc gcggcgtcgt cgtccatttc
     4921 cgcggctgca gcggggagcc caaccgtttg ccacgcgcct atcactccgg cgacgccgat
     4981 gaaatcgcat ggatactgca gcgcctgcga aggctcgatg cggcgtttcc attgttcgcg
     5041 gccggcgtct cgcttggcgg caacgcgctg ctgaagtggc ttggacgcga acaaaccgct
     5101 gcgcgcgaga tgatcgatgc cgccgcagcg gtgtccgcac cactcgacct gatgaccgcc
     5161 ggagaccgct tgggcgcagg cttgaatcgg ctctacggcc tgcatttttt gcaaacactg
     5221 aagccgaaaa gcctcgcgaa gctcgaacgc tttccgggtc tgtacgatgg ccaagtggtg
     5281 agcaaggcgc gaaccctgcg cgagtttgac aacgtcgtaa ccgcacccct gcatggatac
     5341 cgcgacaccg acgactattg gcagcgcgca agcagcaaac cagatcttcg cggcattgcc
     5401 gtgccgacac tgatcgtgca cgcgcgcaac gatccttttc tgcccggccc gcatcttcca
     5461 accgccagcg acgtttctca gagcgttaca ctggattttc cagagcacgg cggccatacc
     5521 ggattcgcct cggggccctt tcccggcaat ctcgactggc tgccgcagcg gctacttgcg
     5581 tttttctcga cacgcatttc agcgcaccgc tgacgtaatc gctctgagcg cgaggccgcc
     5641 acggcagtcg tcggtgtggg ataatcggcg gcaaatcgac cttcggtgcc tttcatgtca
     5701 ttgcctgctt ctgaaatttt caaggcctac gatatccgcg gtgtagtcgg caagacgctc
     5761 acaccgcaag taactgagat ggtcggtcgt gcagtaggct cacaggcgcg cgaactcggc
     5821 caatcgcgcc tttgcatcgg acgcgatggc cggttgtccg gtccggattt gtcggaagcg
     5881 ctggcgcgag gcgtccaatc ggcaggagtc gatgtgattg acattggccg ggtgacaacg
     5941 ccgatgctct atttcgccac gcatcatttg cagaccggct ccggcgtgat ggtcaccggc
     6001 agccacaacc cgccggacta caacggcttg aagatgatga ttgccgggga aacgctgtcc
     6061 ggcgaagcaa ttcaaggcct gcgccggcgc atcgagtcag gcaaatttgc ggctggcact
     6121 ggaggctaca gccagacgca agtcgccgag gcttatctgc agcgcattac gggtgacatc
     6181 aagcttaaac gcaaaatgcg tattgccgtg gattgcggaa acggcgtgcc gggtgctttc
     6241 gccccggaac tgtttcgccg gttgggttgc gaggttgagg agctgttctg cgatgttgat
     6301 ggcacatttc cgaatcatca tcccgatcct tcgcaaccgg aaaaccttga ggatctgatc
     6361 gctgcgttac gcaaggggtc cgcagaaatc gggcttgcct tcgatggcga cggcgaccgg
     6421 cttggcgtgg tcaccaagca gggcaaagtc atctttcccg atcgtcaact gatgctgttc
     6481 gctgcggatg tactgaagcg taatcccggc gccgaaatac tgttcgacgt gaagtgcaca
     6541 cgcaatcttg cgccgtggat ccgccaacac ggcggcaaac ccacgatgtg gaagaccggg
     6601 cattcgctga tcaaagccaa gatgaaggaa accggtgccc tgctggcggg cgaaatgagc
     6661 ggccacattt tcttcaagga acgctggtat ggctttgatg acggcgccta tgccggcgct
     6721 cgcctgctgg aattcctaag cagcgtgcaa gatatcaagt ccacttttga ttcacttccc
     6781 gattcgatca atacgccgga attgcagatc aggcttgccg agggcgaaaa ttatgcattg
     6841 atcgaccagc tgcagaagaa cgcaaacttc tccagcgcgc gcgaggtcat caaaatcgac
     6901 ggcttgcgtg ttgaatatgc cgatggcttt gggctcgccc gctcttccaa taccactccg
     6961 gttgtcgtgt tgcgcttcga ggcagacaat gacaatgcac tcaaacgcat tcaggaggaa
     7021 ttccggggag tactgcagaa ggctgctccc gaggcgaaat tgccgttcta gcgctgctgc
     7081 tgttcgagca gcttcttcag attatcgagt ccgcttttgt aggcgccgtt gaggaaatcc
     7141 agcgccgcct tgtcgtcctg tcccgcgggt ggttgatctg tccagtaagc aagccgcttg
     7201 aaccttccgc gccactcaac gatggaacgg ccgctgccgg catccttgac cgccacctcg
     7261 gagtaatagt cggccgccct caccacgccg tcgacatagg tgtaagccat gcgccggttg
     7321 tatggatcgt agtcgagcag cttctcggtg acctgggtgc catcaccgcg acgacgcagc
     7381 aggcggatcg cgccggtctc gttgcggtcg tgcagcacca ggcggctcga ttccgtgccc
     7441 ggcgcccagc gctggatgcc gccaaaatcg ccgatcaccg cccagacctc gtccgccgga
     7501 gcattgatcg tgatcgtatg gaaaatggtc aggtcctgcg catcaccgaa cgcaggcaat
     7561 gcgctcgcca caagcaacat ggccgccgtc cacttcgtca gtttgttcat gtgtcctccg
     7621 ttgagggcta ttcgccgggt gttatgcgat tctttgtctg acccagtcgg gtacgctatc
     7681 gagcgccatg cgcaacttcg ccggctccgt accccctgcc tgcgccatgt ccggcctgcc
     7741 accgcctttg cccccgactt gctgggcaac gaagttgacc aattcacccg cctttacttt
     7801 cgcagtcaaa tcggaggtca cacccgcaat cagagtcacc cggtcgcctt caattgcgcc
     7861 gagcacgatg gccgcggatt tcagcttgtc cttcagcttg tccatggctt cacgcaacga
     7921 cctggcatct gcgccgtcca gccttgcggc cagcactctt gacgcaccga cggccaccgc
     7981 ctgatcggca agatcatcgc cttgtgtgtt ggcgagcttg gacttcagcc gcgacaattc
     8041 cttctccagg gatttcacgt tgtccaggat ctgtcccagt ttttgcgtca attcctgcgg
     8101 ctgcaccttc agcacctggg ccgcctggtt gagccgggtt tcctggtcgt gcacccaggc
     8161 cacggcaccc ttggccgtca ccgcttctac gcgccggatg ccggaagcca cgcctgactc
     8221 ggacacgatc ttgaaaaaac cgatgtcacc cgtacggcgc acatgcgtgc cgccgcacag
     8281 ctcagtggaa aaatctccca tgctcagtac gcgcacttcg tcaccatatt tttcgccaaa
     8341 cagcgccatc gcaccggcct tgatcgcgtc atcgtatttc atgcgcctgg tttcgaccgg
     8401 gtggttttcg cgaatctccc ggttgaccag atcttccact tcgctgatct gctgcgcagt
     8461 caacggcgcg tgatgcgaaa aatcgaaccg ggtacggttg gcgtcgacca gcgatccctt
     8521 ctgggtcaca tgttccccga gcacctttct gagcgcggca tgcatgaggt gcgtagcgga
     8581 atggttgtat gcagccaatg cgcgattcgt ggcgtccacc tgcgccgtga ccgcatcgcc
     8641 gacgctcaac cggcctgccg tcaccttgcc gtgatgcccg aatacttcgg cctggatttt
     8701 ccgggtatcg gcgacgtgga agccgccatt tccggacgtg atttccccct ggtcgccaac
     8761 ttgcccaccg gactctgcat agaacggtgt gcggtcgagc acgaccactg catcttcgcc
     8821 cgcgctgact gactgcacct gcgagccgtc tctgtacagc gctattacat gtgcttccag
     8881 gcgcagcgtc tcgtagccgt ggaattcggt cgcgcgaccg gaatagtcta cgctggagtg
     8941 cacggtgaac ttcgatgccg ctcgggcgcg ttcgcgctgt cgctgcatgg cttcttcaaa
     9001 accggcgtgg tcgatcatga cgccgcgctc gcgcgcgatg tctgcggtga gatcgaccgg
     9061 gaagccgaac gtatcgtaca gctgaaatac agtttcgcca tccagcattc tgtcttcgcg
     9121 atgcaatgcg ttctcgagca ccttcatgcc gttttccagc gtttcggcga agcgctcttc
     9181 ctcctgtttc agcacatccg ttacgcgccc ctgagccgtc gtcagctcgg ggtaggcgtc
     9241 gcccatcacg cgggaaagtt cggcaaccag cttgtggaag aaaggtttgg tcttcccaag
     9301 cttgtaacca tggcgaattg cccggcggat aatccggcga agcacataac cccggccttc
     9361 gtttcctgga atgaccccgt cggtaatcag gaatgagcag gctcggatat ggtccgcaat
     9421 gacgcgcagc gaactggagt tcagatcctt ggccccggtt actttcgcgg ccgccctgat
     9481 gagatcctgg aacaggtcga tttcgtaatt gctatggact ccctgcaaca cagccgcaat
     9541 gcgctccagg cccatgccgg tatcgactga cggcttgggc agtgggtgca tggtgccaga
     9601 atcgtcacga ttgaactgca tgaacacgag attccagatt tcgatataac ggtcgccgtc
     9661 ggcatcgacc gatccaggag gaccgccggg aataccgggc ccatggtcgt agaaaatctc
     9721 ggtgcacggg ccgcagggac cggtatcgcc catctgccag aaattatcgc tctggaattt
     9781 ctgaccgcct ggcttgtcac cgatgcgcgc aatgcgctcc ctcggaacct tgatttcgtt
     9841 ggcccagatg tcataagcct catcatccgt ctcgtacacc gtgacccaca ggcgctttga
     9901 atcgagcccg tattccctgg tcagcagttc ccaggcgaag cggatggcgt cgcgtttgaa
     9961 ataatcgccg aaactgaaat tgccgagcat ttcgaaaaac gtgtgatgcc gggcggtata
    10021 gccgacgttc tcaagatcat tatgtttgcc gcctgcgcga acgcaacgct gagacgtggt
    10081 cgcgcgcaca taaggacgct tgtcgtgccc tagaaataca tccttgaact gcaccatacc
    10141 ggcattggtg aacaacagcg tcgggtcgtt ggccggtacc agcgggctgg acgcgacaat
    10201 ggtatggcct tgccgggcga agtactggag gaaccgcgtg cggatttcgc tgcttttcat
    10261 tgcgtgagga ccaaagattc aaacggccgc gctccaatgc gcggcacata cgtaacgaat
    10321 tattgcatta cagacagcgg aggggctact actcgtcgtt gcacagcacc tgatgcacga
    10381 tttccgccga aaaaccgcgg ctggcgagaa agcgggactg cttcgctcgc tccgccagcg
    10441 ttgcaggaag cttgccgaat ttctttcgcc aaaccgtccg cgcggcttct atttcactgt
    10501 ttttcagctg cggctgcaac gccgcgagag cgtcctcgga cacgcccttg tcgcgcaatt
    10561 cacgcaaaat gcgggccgcg ccgaacctgc gtctgcgcac ggtcgtcacg tgctccacga
    10621 aacgagcctc ggacaaccac ccgtgctttt ccagatcgtc gagcaaaggc cccagatcgt
    10681 ccgggctttc ggcgaaaggc cgcagcttag cctccagctc atcgcgcgaa tgttcgcgcc
    10741 gcgccagtgc tttgagtgcg cgtgcgcgca aactgccagg caacggcgca ggcaatcctc
    10801 gcgttactcc ggttccaaca caggcgataa gggtttttcc gccacgtgcg atcggatctt
    10861 gttctctatc tccttcgcca cgtcaggatg ttccttcaga aactcgcgcg tgttgtcttt
    10921 accctggccg atacggtcct tgccatagct gtaccacgcc ccggactttt cgatcatctt
    10981 gtagagcaca cccagctcaa tcagttcgcc ctccagcgaa ataccttcac cgtacagaat
    11041 atcgaattca gcctggcgga acggcggcgc caccttgttc ttgactacct tggcacgggt
    11101 ttcgtttccg accacttctt cacccttctt gatggagccg atgcggcgga tgtctatgcg
    11161 gacggacgca tagaacttca acgcattgcc gccagtcgtg gtttccggat tgccgaacat
    11221 gacgccgatt ttcatgcgaa tctggttgat gaatatcacc agcgtgttgg agcgctttat
    11281 attggcggtc aatttgcgca gcgcctggga catcagccgc gcctgcagac ccatttgcgg
    11341 ttcccccatt tcgccttcta tttccgcctt cggcgtcagg gcggccaccg agtccacaac
    11401 gacgacgtcc actgacccgg agcgcaccag catgtcggca atttccagag cctgttcgcc
    11461 gttgtcgggt tgcgaaatca gaagttccga aacattgcat ccgagtttct gcgcatactg
    11521 gggatcgagc gcatgctccg cgtcaatgaa agcagctgta ccgccgagtt tctgcatctg
    11581 ggcgatgacc gacagcgtca gcgtggtttt gcctgaagac tccggcccgt aaatttcgac
    11641 aatgcgtcca cgcggaagac cgccgatacc gagcgcgagg tccaggccaa gcgagccggt
    11701 ggaaacgact tccacgtcct tggcgatgtc cttctcgcca agacgcatga tcgagccctt
    11761 gccgaattgt ttttcaatct gcgagagggc tgcggcgagc gctttgctcc gattttcgtc
    11821 catgcacgtt tcctctgtga tcaacataga attattgcat taaagctgaa ttgaatcgcc
    11881 gccatggaag ccacctctct aaagtcgctc gctgatttga cgcatccgga ttcgcttata
    11941 aatgccctgc actggcgcac acacttttgc accggtcgaa ttatgatact gtaattatat
    12001 acagtcatgc aaaccgtttg cacggccacg ctgcaaggtg ctgatccgta taggaacgtg
    12061 aaaaatcgta acggagcttc aggactgcgg atggagcagc gcgagtattc cgtccaaggc
    12121 tgcgatgacg ctctggcggc gcacggcgtc ccggtcgccc ggaaaatgca tcgcctgcgc
    12181 ccgggtgctg acgccctttt gtccccacgc cagcaatacc gtgccaaccg gcttgctgat
    12241 ggagccacca tcgggaccgg caatgccgct gactgcgacg gcaacctgcg cgcggcttct
    12301 ggccagtgcg ccagccacca tttcgagcac ggtttgctcg gatacggcac caaattggct
    12361 caatgttgcg tgccgcacgc caagcatgtc gtgcttcgcg tcattggtgt aagtaataaa
    12421 gccacggtcg aaccaaccgg aactgccggg caccatcgtg accgcctgac cgatccagcc
    12481 gccagtgcag gactcggcag tcgacatcat gaacttgtac gcctgcaacg tctcgccgac
    12541 ccgttccgcc aactcgtaaa tttcctcgtc catgggctag ctgaaaatga attatccgaa
    12601 caataatttg cagcctgcca ggcaaagcag tgtataaaac gcggcaagca agtcatcgaa
    12661 catgacaccc aatccattgc ggacagttcg atcaacatac cggatggggg caggcttcag
    12721 aatatcgaac agccggaacg cgagaaacgc aaatgcctgc cacacgaggg aagccggcgt
    12781 aaagaacaat acgatcagga acgcaactgt ctcgtcccag accatgccgc tgtgatcgtg
    12841 cacgcggagt gccttgccgg tgacctggca tgcccacaca ccgagcgcaa acagcagcag
    12901 catcagcaat gcaaataccg gctcacctag ccggtatacc agccaggtat acagcgggaa
    12961 tgccagcaac gtaccgaatg tgccgggtgc aaccggaatc agcccaacac cgaaaccgaa
    13021 agaaacaaaa tgcgccgggt gcgaatacac gaaacgcact gtgggccgtg ccggagactc
    13081 agcgaaagtg gtcaaatccg ttatcctcta cggacagcgg gccgcccgat gcatcgagca
    13141 cgctgacctt gttgccgtcg acaatgctgc ctatgcgtgt cagctcgatt tccagagttg
    13201 ccgatagcgc cgttatttcg gcggcacgcg acggcgaggc agtaaagcac agttcgtagt
    13261 catcgccacc cgtgagcatt gcccgggtaa cggaaggctg atggcgcaaa ggctgaagga
    13321 ctgcagcaca cggcacggcg gcggaatgaa tctgggcacc gacacctgaa cgctcgcaga
    13381 tgtgcgccag atcgcccacc aatccatccg aaacatcgat tgcgctattg gcgagggcac
    13441 tgagttctaa ccccagtgca acgcgcggcg ccggccagtc caaccgcaca cggcattgtt
    13501 cgaatacggc cggtggcaga ttcatttcac cacgccggtg tgcgacggcc gccgctgcgg
    13561 cacccagttc accggacacc cagatatcgt caccgatttt cgctccgtcc cgacgcagag
    13621 cctggccgct ggcgacctcg cccatgatct gcacgcagat gttgcgcggc ccgcgtgttg
    13681 tgtcgccgcc gatcaaatca acgccgtgct tgtcggcaag cgcaaaaaag ccgcgcgaga
    13741 atgcttccag ccattgtgtg tcagcttcgg gaatggcaag cgccaacatg gcccatctgg
    13801 gcatggcgcc cattgccgca agatccgata aattgaccgc cagcgcctta tgaccaagtg
    13861 cctgtggatc cacatctgca aaaaaatgca cgccttccac caacatgtca gtcgatgcag
    13921 ccaattcgct gcccgcagtc acccgcagca acgctgcatc gtcgccgatg cccagttcgg
    13981 tgtgacgggg cgcgcgcgtg aaatagcgcg agatcacatc gaattccgac aacgctgggc
    14041 ggctggcggc aggcctggtc atctgcggaa aatactatga aaggcgacgt gccgatcagc
    14101 tgtggtcgag ttttttcaga aacgcctgat actcatcttc cttcatggtg aagctgtgtt
    14161 ccgcgagcgg gaactgtccc ttgtgaacat cgtcctgaaa ttctgcgagc gctttgccaa
    14221 tgacatccca gcagttcgcg taacgcttga cgaatttagg tgtgaagcgg tcgaacagtc
    14281 cgaccatatc gtgcagcacc agattttggc cgtcgcagtg cggtcctgcc ccgatgccga
    14341 tggtcggtat agtcagtttt tgcgtgatga cctgtgcgac ccgatccgga atcgcctcga
    14401 gcacgatcgc gaagcagcct gcct
//
LOCUS       DATMZQ010000348         4289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3595301, whole genome shotgun sequence.
ACCESSION   DATMZQ010000348 DATMZQ010000000
VERSION     DATMZQ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3595301"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..374
                     /locus_tag="VN496_11130"
     CDS             51..374
                     /locus_tag="VN496_11130"
                     /inference="COORDINATES: protein motif:HMM:NF027450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HXF17551.1"
                     /translation="MERMTFYAQSVLCRCKMLLEYFGEAVEWDRCGVCDNCERPVVAV
                     TREPAPSKPAAWADLARGERVSVPRYGRGIVDAAAGDRVTVSFPNGEPRGFLRPYVSR
                     VSRRH"
     gene            complement(398..1297)
                     /locus_tag="VN496_11135"
     CDS             complement(398..1297)
                     /locus_tag="VN496_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17552.1"
                     /translation="MAISLKPLDQQVIVITGASSGIGLAAAEEAAEQGAKLVLASRSE
                     ATLATIVSEINSTVSEAIYVVADVGDRAQVEKIADAAIDHFGRIDTWINDAGGSIYGR
                     LDVVSDKDNRRLFDTNFWGVVNGSLVALAHLRQHGGALINIGSEVSEAVIPLQGMYSA
                     SKHAVKGFTDALRIEVQELDKAPVSITLIQPTAVDTPFPQHAKNYMDREPKLPTPQID
                     PHEVARAIMKAATEPTRDIKVGAMSKINTAVQNLLPAMAQKIEAKQAGRQHYDEPPRN
                     PEGALFKPGGTGQVYGSGGAAHH"
     gene            1547..1747
                     /locus_tag="VN496_11140"
     CDS             1547..1747
                     /locus_tag="VN496_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17553.1"
                     /translation="MSTNGQKTPPHDPEDDDPRAGVEADEATGQIPVGPVRKQVLPPD
                     EQGSSAAHEPFGAEDHERKEKR"
     gene            complement(1777..2517)
                     /locus_tag="VN496_11145"
     CDS             complement(1777..2517)
                     /locus_tag="VN496_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXF17554.1"
                     /translation="MKIATWNVNGIRARQAQFCEWVERDRPDVVCLQELKAQPAQIPQ
                     QCHHIDYHVYWHGLRAYSGVSLHVRKGLFEQEPVFSHPDFDMETRIVQATIGRLVLAS
                     VYVPNGGKDYPAKLAFMTQLAAWAKRLHDEGLELVLCGDMNITRTDLDVHPRERKPGI
                     IGQREEERALFEALLGEHLVDVGRKLDPDNPNLFTWWAPWRNMRQRNIGWRLDYILAC
                     PSIADRAAQCLVLAEVGTSDHAPVMMTM"
     gene            2767..3738
                     /locus_tag="VN496_11150"
     CDS             2767..3738
                     /locus_tag="VN496_11150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXF17555.1"
                     /translation="MESALLAQMVSSSDILDARILLVDDNEENLHLLDQILRMAGYTA
                     IDLTTNPREVCDLHRKNDYALILLDLQMPELNGFEVMEGLKQIELEAGSYLPVLAITA
                     NPDHKLRALEAGAKDFISKPFDPPELVRRVYNMLEVRLLHQETRKHGKMLESLALQDP
                     LTGLANRRLLVDRMSMAVAQARRNKAHMAVVFMDLDGFKQINDTLGHGAGDTLLKTVA
                     RRLRGTVREEDTVARLGGDEFVMSLPHIHGEEDAARMAAKIIDAVSQPLQIEGRTVRV
                     TTSAGVGIFPENGKDADTLMKAADGALYEAKRAGKNNYRIASKPDLR"
     gene            complement(3778..>4289)
                     /locus_tag="VN496_11155"
     CDS             complement(3778..>4289)
                     /locus_tag="VN496_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF17556.1"
                     /translation="FTGTIYEILSAPVSAVEIVLAYVGAAATKSVILGLVILATATLF
                     VPLHIAHPVWMIAFLVMTAAAFSLFGFIIGIWAKGFEQLQFIPMLIITPLTFLGGAFY
                     SIDMLPPAWRTVSLFNPIVYLISGFRWSFYETADVGVGLSLTMTLLFFALCLGAVVWI
                     FRTGYRLKT"
BASE COUNT          899 a         1222 c         1284 g          884 t
ORIGIN      
        1 cgtgtgagtg ggcaccggcc gcgtagcagc tggaacaggt tgcgcgaggt ctggaacgaa
       61 tgacgtttta tgcgcagagt gtgctgtgcc gatgcaaaat gctcttggaa tattttggcg
      121 aagcagtgga atgggatcgc tgtggcgtgt gcgataactg cgagcgtccc gtcgtcgcgg
      181 tcacgcgcga accggcgcca tcaaagcctg cggcgtgggc cgatcttgca cgcggcgaac
      241 gcgtcagcgt gccaaggtac gggcgcggca ttgtggacgc tgccgccggc gaccgggtaa
      301 ctgtcagttt cccgaacggc gaaccccggg gcttcctgcg tccttatgtt tcccgcgtct
      361 cgcgccgcca ttgactcaac tggctgacgc tgagtcgtca atgatgtgct gcaccgccgc
      421 tgccgtacac ctgacccgtg ccgcccggct tgaatagtgc gccttcggga ttgcggggcg
      481 gttcatcata atgttgccgg ccggcttgct ttgcctcgat tttttgcgcc atcgcaggca
      541 acaggttctg gacggcagtg ttgatttttg acattgctcc caccttgata tcgcgcgttg
      601 gttcggtggc tgccttcata attgcgcgcg caacctcatg cgggtcgatt tgcggcgtcg
      661 gcaatttcgg ttcccggtcc atgtagttct tcgcatgctg cgggaaagga gtatcaaccg
      721 cggtcggttg aatcagcgtt atggacaccg gcgccttgtc caattcctgc acttcgatac
      781 gcagtgcatc ggtgaaccct ttcactgcgt gctttgaagc tgaatacatg ccctgcaacg
      841 ggatgactgc ttcggacact tcgctgccga tgttgatgag cgcgccgcca tgctggcgta
      901 aatgggcaag agcaaccagc gaaccgttga ccacgcccca gaaattggta tcgaacagac
      961 gcctgttgtc cttatcggac accacatcca gccgcccgta aatggagcca cccgcatcgt
     1021 ttatccaggt gtcgatgcgc ccaaagtgat cgatggcggc atcggcgatt ttctcgacct
     1081 gtgcgcgatc gccgacgtcg gctaccacgt agatcgcctc gctcacggtg gagttgatct
     1141 ccgaaacgat cgtggcgagg gttgcttcgc tgcgcgaggc gagtaccagt tttgcgcctt
     1201 gctcggcggc ctcttctgct gcggccaagc caatgccgct tgacgcgccc gtgatgacta
     1261 tgacttgctg gtcgaggggt ttcaatgaaa tagccatgag tattgtcctg attcagtcgt
     1321 ttgctggaaa aatgggcgag cgttgttcgc gcttgtccgc tgcgttgttg tgctcagatg
     1381 accgcgccgt tcgcgcatag gttcccggcc aacgacggac gcgtgaaatc gcgcgctatt
     1441 cagcgcacca gcggcccgat atgagcgctg aaattcttat tactcttttt ggcaccgagc
     1501 ttgctttgtt gccttcaatc gaacgaactg aaagcagagc atcgcgatgt caactaatgg
     1561 gcagaaaaca cctccgcacg atcccgagga cgatgaccct cgcgccgggg tagaggctga
     1621 cgaagcaacc gggcaaatcc cggtaggtcc ggtacgcaag caggtgcttc cgccagatga
     1681 acaggggtct tcggctgcgc acgagccgtt cggcgctgag gaccacgagc gcaaagaaaa
     1741 acgctgaact cgacgttgaa gacgggcagg actctgctac atggtcatca tcactggcgc
     1801 atggtcactg gttccaactt ctgccagcac taggcactgc gcagcgcgat ctgcaatgga
     1861 tgggcaggcc agaatgtaat ccagccgcca tccgatattg cgttgtcgca tgttgcgcca
     1921 gggcgcccac caggtgaaca gattgggatt gtccgggtcg agcttgcggc caacgtccac
     1981 aagatgctcc ccgagcaagg cctcaaacaa ggcgcgctcc tcctcccgtt ggccaatgat
     2041 gccgggcttg cgttctctgg gatgcacgtc aagatcagtg cgggtgatgt tcatatcacc
     2101 gcatagcacc agttcaaggc cctcgtcatg caaccgcttt gcccaggcgg cgagctgagt
     2161 catgaaggcc agcttggccg gataatcctt gccaccgttg ggcacgtaca ccgaggcgag
     2221 cacaagcctg cctatggtgg cctgtacgat gcgggtttcc atgtcgaaat cgggatggct
     2281 gaataccggc tcttgttcga acagcccttt cctgacatgc agcgacaccc ccgagtaagc
     2341 gcgcagtccg tgccagtaga cgtgatagtc gatatggtgg cattgctgcg gaatctgtgc
     2401 cggctgggct ttcaattcct gcaggcacac cacatcagga cggtctcgct ccacccattc
     2461 gcaaaattgg gcttggcggg cgcgaatacc gtttacgttc caggtggcga tcttcatcta
     2521 aggcgaaata aacaacgaga ccggttgcgc ttgatgtgct cctgtcgccc gacagcacac
     2581 tggccgaaat tggccagggc cctaaccgca agcatgccgg tcggaccatg gattgtcgca
     2641 ttgagcatgg caggccacgc gtaactttgg gatattttcc gggttccggc ggaactttct
     2701 gaggcgctgg cggcccatct gtgtgccttc ccggaatggg cctggaaaac gcctttcacg
     2761 ttaacgatgg agagtgcttt actggcgcag atggtgagtt cctccgacat tctcgatgcc
     2821 cgtattcttc ttgtagacga taacgaagaa aacctgcatt tgctggatca aatacttcgc
     2881 atggcgggtt ataccgccat cgatttgacg acaaatccac gcgaagtttg cgacttgcat
     2941 cgcaagaatg actacgcgct gattctgctc gacctgcaaa tgccggagct gaacggtttc
     3001 gaggtcatgg agggattgaa gcaaatcgaa ctggaggcgg gcagctatct gccggtactc
     3061 gccatcaccg ccaatcccga ccacaagttg cgcgccctcg aagcgggcgc caaggatttc
     3121 atcagcaaac cattcgatcc tcccgaactg gtcaggcgcg tgtacaacat gctcgaggtc
     3181 cggctgctgc atcaggagac ccgcaagcac ggcaagatgc tggagtctct ggcgttgcag
     3241 gatccgctta cgggcctggc gaaccggcga ctgttggtgg atcgcatgtc gatggcggtg
     3301 gcgcaggcca gaaggaacaa ggcccacatg gcggtggtct tcatggatct ggacggattc
     3361 aagcagatca acgacaccct gggccacggc gccggagaca cgttgctaaa aaccgtcgcg
     3421 cgtcggcttc gcggcacggt acgggaagaa gatactgtgg cccgcctcgg aggtgatgag
     3481 ttcgtgatgt cgctgccgca tatccatggc gaggaagatg cagcccggat ggcggcaaag
     3541 atcatcgacg cggtctccca accattgcaa atcgaaggtc gtaccgtgcg cgtcacgacc
     3601 agcgcgggcg ttggcatttt tccggagaac ggtaaagatg ctgacacgct catgaaggcc
     3661 gcggacggcg cgctgtacga agccaagcga gcgggcaaga acaactatcg gatcgcaagt
     3721 aaaccggact tacgctgacc caccggattc agcggctcgt cttgccgaag cggcatctca
     3781 tgtcttcagc cggtagccgg ttctgaaaat ccatactacg gcaccgaggc agagcgcaaa
     3841 gaacaatagg gtcatcgtaa ggctcagccc cacgccgacg tcggccgtct cgtagaaact
     3901 ccagcggaag ccgctgatca gatagacgat cgggttgaac agactgaccg tgcgccatgc
     3961 tggcggcagc atgtcgatcg agtagaaggc gccgcccaga aacgtcaacg gcgtgatgat
     4021 tagcattggg atgaactgta gctgttcgaa gcccttggcc catatgccga tgatgaaacc
     4081 aaacaaactg aaggccgccg cagtcatgac cagaaacgcg atcatccaga ccgggtgcgc
     4141 aatgtgcaaa ggcacgaaca aagtggcagt ggccaggatg accagcccga gaataactga
     4201 tttggtcgcg gccgcgccga cgtaggcgag cacaatctcc accgccgata cgggcgcgga
     4261 caggatctcg tatatcgtgc cggtaaatt
//
LOCUS       DATMZQ010000349         6813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4284940, whole genome shotgun sequence.
ACCESSION   DATMZQ010000349 DATMZQ010000000
VERSION     DATMZQ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4284940"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..755)
                     /locus_tag="VN496_11160"
     CDS             complement(207..755)
                     /locus_tag="VN496_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17557.1"
                     /translation="MNKPILPAIAVPSVRWIVFAASLLIAGAACYTAKVHAAEPFNRG
                     TVSGSLFIGSGHALDQTYTTLGVGLGYMVSDGLMAGVTGEAWFGNDPSIYKLTPELRY
                     TFTKVERVKPYVGVFVSRMFFSNTFDDRNTYGGRAGIYLPFSSNAAANLGVVYEKLSD
                     CDEATYRSCSQTYPEVGVLVSF"
     gene            917..2710
                     /locus_tag="VN496_11165"
     CDS             917..2710
                     /locus_tag="VN496_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXF17558.1"
                     /translation="MNSKAQSFFSRQVSSSRMAANLRALSILMQFAKPYRRRMLFFAA
                     ALIVSAGCFLVIGQGLKQVVDRGFSHGDPAALNQGLFFLLGVIVVMAGATWVRFYLIT
                     WLGERVIADIRRTVFSHLLNLSPAWFEQTRTGEMISRLTTDTALLEQIIGSSLSMALR
                     NALLGAGALAMLMLTSWKLTLLVLLTVPVVIVPIVLFGRRVRKLARASQDRVADIGAY
                     VDEALHEIRTLQAYGHEDTDRELFARRIEAAFDTARRRIRVRAWLISAVIVLVFAGIG
                     VILWIGGHDVLAGKLTAGELSAFVFYAAMVAAAAAALSEVMGDVQRGAGAAERLIEIL
                     DTKPEIVVPDNALPLPHPVVGSVELDAVIFHYPSRPAIAALQDFSLRIAPGEKLALVG
                     PSGAGKSTVFQLLLRFYDPQHGTIRIDGVDIRRVDPRRLRERIALVAQEPAIFAASVT
                     ENVRYGRPAASDSDVKQACHAAFATEFIDQLPHGFDTYLGERGVRLSGGQRQRLAIAR
                     AVLSDRPILLLDEATSALDSESERMVQAALQRLMESRTTLIIAHRLSTVKSVDRIAVI
                     DGGRLIGEGTHEALLASNPLYARLAALQFGA"
     gene            complement(2792..3301)
                     /locus_tag="VN496_11170"
     CDS             complement(2792..3301)
                     /locus_tag="VN496_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXF17559.1"
                     /translation="MNRIALAALMLLAVCAYATAATLEGRVVGVSDGDTVTVLTSENR
                     QFRIRLSGIDAPEKKQAFGTHAKETLARQLFGQAVVIEWSKTDRYGRILGKIEVGGVD
                     ANLEQLRAGSAWVYTQYLRELAPADRVLYLDAQRQAQSEHRGLWRDSEPEPPWQWRHE
                     LRAHHRHGS"
     gene            3440..4525
                     /locus_tag="VN496_11175"
     CDS             3440..4525
                     /locus_tag="VN496_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HXF17560.1"
                     /translation="MSSRLFTPFEMRGVTLPNRIVVSPMCQYMAEEGSANDWHLMHLG
                     QFAMGAGGLVITEATHVSPVGRITPRCLGLYSDDNERTLGHVVDFCRKYGVVRMGIQL
                     AHAGRKGSTHTPFQGGKPLNSNEGAWTTVAPSPLPYAPGWHVPEPLSESGLADTKQQF
                     VESARRALRIGFDLIELHSAHGYLLHQFLSPISNQRTDRYGGALENRMRFPLEVFEAV
                     RAVWPQDRVLGVRVSATDWIEGGWTPEETVVYARELKNRGCDFIDASSGALDPRQQIP
                     FGPGYNVPFAEKIKKFSGIATMAVGLITKPAQAEEIISSGKADLIAIARAAMDDPRWA
                     WHAARELGDEAAYAPNYVRASPAVWKP"
     gene            4557..5426
                     /locus_tag="VN496_11180"
     CDS             4557..5426
                     /locus_tag="VN496_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXF17561.1"
                     /translation="MSAIAQVMQGRPRDLGGLQVRRILPYGGGRMVGPFIFLDHMGPV
                     QFAPGAGIDVRPHPHVCLATVTYLFEGEIEHRDSLGVVQTIRPGEVNWMTAGNGIAHS
                     ERTGATLRAQGHKLHGLQSWVALPVADEETLPAFNHFSTSQLPVREQDGVVVRLIAGD
                     AFGARSPVPVFSSMFYADVVMQAGASVTVPDEHAQRAAYIVEGEIEIERRKFGEGALL
                     LFEEGEPARLRASGASRVAVLGGAPLDGERHIWWNFVASSPERIERAKRDWANGMFAR
                     IPGETEFIPLPPS"
     gene            5541..6179
                     /locus_tag="VN496_11185"
     CDS             5541..6179
                     /locus_tag="VN496_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXF17562.1"
                     /translation="MKLYFDPISVNCRKVVAGFGLMGVDYESAHVDYFKAEQKAPAYL
                     QINPNGAIPALVDGEMQLWESNAILQYAADKNEAFGYYPKDLKMRADINRWLLWEAAH
                     WFPSNYVYLVENVVKPLLKAQPDKAIIDKEAPNWHRLAGILDARLAKHKWLCGDKVTL
                     ADIAVAAPLHLHPYQKVPLDAHPNLKRWMTQGIEQLPCWKATDAAKLLGLAA"
     gene            6228..>6813
                     /locus_tag="VN496_11190"
     CDS             6228..>6813
                     /locus_tag="VN496_11190"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HXF17563.1"
                     /translation="MRRDKSVRASFNYTRDDGTVPEVYFYEPPAGTVVHPPGTDAREM
                     AITDGWSRADGFSLDREGFALKEFRNPFDRFDDDEAVRSQFYESVAEFVRSSVGAKRV
                     MVFDHTIRSKVNEQQQTAEHTTTQRAPVMIVHCDYTPVSGPLRVRQLLPGEAEGLLKR
                     RVAFFNFWKPLKRRVEEKPLAMCEVTSATQDDMIK"
BASE COUNT         1328 a         1998 c         2089 g         1398 t
ORIGIN      
        1 acagatatca aatcgtcacg tattgaagcc taagacggct tcgatgatca cgaagttccg
       61 tgttcagtga tttcccgaag tgcaagcggg tggcgggtgg caagtaccgc ttggcgtttg
      121 ccgacagccg cacgaatgta atcacgcgcc tgcacgcaac gtgcccgccc gattagtatc
      181 ccagtcacat gcggcgggcg actgcgtcag aaggaaacca atacacccac ctcgggatag
      241 gtctgggaac aactccgata ggttgcctcg tcgcaatcgg aaagtttttc gtaaaccacg
      301 ccgagattcg ctgcagcatt cgaactgaac ggcagataaa tgccggcgcg cccgccatag
      361 gtattgcggt cgtcgaaggt gttgctgaag aacatgcggg aaacgaatac gccgacataa
      421 ggcttcaccc gttccacctt cgtgaaggtg tagcgcagtt ccggcgtgag cttgtagata
      481 ctcggatcgt tgccaaacca ggcttctccg gtgacgcccg ccatcaggcc gtccgatacc
      541 atataaccga ggccaacacc caacgtcgta tacgtctggt caagcgcgtg accgctgccg
      601 atgaaaagcg atccggaaac agtgccacgg ttgaatggtt cagccgcgtg aaccttggcg
      661 gtgtagcagg ctgcccccgc gatcaacaac gaagcggcaa aaacaatcca gcgtacggaa
      721 gggaccgcaa tcgcgggcaa tattggtttg ttcatgattc ccccaatgtt ttcggctaca
      781 ttgccgtgac ttcacgcaga agctacatca tacaagctcc gtgcgcgtga gcgaaacgct
      841 aaacgcgcct tacgtcgcgt tagttcagcc cacatgtcgc tcctggatcc ggctggggca
      901 attcgaagat agaaacatga acagcaaagc gcaaagtttt ttttcccggc aggtgtcgtc
      961 ctcgcgcatg gccgccaacc tccgcgccct gtcgattctc atgcagtttg ccaagcctta
     1021 ccggcgccgc atgctgtttt ttgcagctgc cctgattgtg tccgccggct gtttcctggt
     1081 gattggccaa ggtctcaaac aagtggtcga ccgcggattt tcacatggcg atcctgcggc
     1141 gctgaatcag ggactgttct ttctgctggg tgtgattgtc gtcatggctg gcgccacgtg
     1201 ggtgcgcttc tatttgatca catggctggg agagcgcgtg attgcggata tccgccgcac
     1261 ggttttctcg catttgctta atttgtcgcc ggcatggttc gagcagacgc gcaccggtga
     1321 aatgatctcg cggctgacga ccgataccgc gctgctggag caaatcatcg gctcgagcct
     1381 gtcgatggcg ttgcgcaacg cgctgcttgg cgcgggggca ctggccatgc tgatgttgac
     1441 cagctggaag ctgacgctgc tggtgttgct gaccgtgcca gtggtcattg ttccgatcgt
     1501 gctgttcggt cgcagggtgc gcaaactcgc gcgcgccagc caggatcgcg tcgccgatat
     1561 tggcgcctac gtcgacgaag cactgcatga aatacgcacg ctgcaggcat atggacacga
     1621 ggatactgac cgcgagctgt tcgcgcggcg tatcgaggcg gcgttcgaca ctgcgcgcag
     1681 gcgtatccgc gtacgcgcct ggctgatctc tgcggtgatc gtcctggtgt tcgccggcat
     1741 cggcgtaatc ctctggattg gcggtcatga cgtgctcgca ggcaagctca ccgcgggaga
     1801 attgtccgcg ttcgttttct atgcggcgat ggtggctgcc gcagcggccg cattaagtga
     1861 agtcatgggc gatgtgcaac ggggcgcggg cgcggctgaa agactgatcg agattctgga
     1921 tacgaagccc gagattgttg taccggataa cgctcttcct ttgccgcatc cggtggtggg
     1981 atcggtggag ctggatgccg tgattttcca ctacccttcg cgcccggcca tcgcggcatt
     2041 gcaggacttt agcctgcgca tcgcgccggg agaaaagctg gcgctggtcg gtccttccgg
     2101 cgcaggcaag tcgaccgtgt tccagcttct tctacgcttt tatgatccac agcacggcac
     2161 cattcgcatc gacggcgtcg atatccggcg cgtagatccc cggcggctgc gggaacgcat
     2221 tgcgctggta gcgcaagagc ctgccatttt cgcagcaagc gttacggaga acgtgcgcta
     2281 tgggcggcca gccgcatccg atagcgacgt gaagcaggcc tgccatgcgg cattcgcaac
     2341 cgagttcatc gatcagttgc cgcacgggtt cgatacttac ctgggagagc gcggcgtgcg
     2401 tttgtccggc ggccagcgtc agcggctcgc cattgcacgc gctgtcctgt ccgaccgccc
     2461 gatcctgttg ctcgatgagg cgacatccgc gctggactcg gaatcggagc gcatggtgca
     2521 agctgcgctg cagcggctga tggaatcgag aacgacgctc atcattgcgc atcgcctgtc
     2581 gacggttaaa agcgtggatc gcattgcggt catcgacggc ggacgattga tcggggaagg
     2641 gacgcacgag gcattactgg cctcgaaccc gttgtatgcc cggcttgcgg cattgcagtt
     2701 cggtgcctga gaacctttgc accgaaaaac cagggacggc tcgctttaac cggctgacgc
     2761 gaagaccgcg tatggcttcg tggtttgaag gctagcttcc gtgccggtga tgcgcgcgca
     2821 gttcatgtcg ccattgccag ggcggttccg gttcgctgtc gcgccataac ccgcgatgct
     2881 cgctttgcgc ctggcgctgg gcgtccagat aaagtacgcg gtctgcaggc gccagctcac
     2941 gcaggtactg cgtgtagacc cacgcagaac ccgcgcgaag ttgttcaaga tttgcatcga
     3001 cgccaccgac ttcgattttg cccaggatgc ggccgtaacg gtcagtcttg gaccattcga
     3061 ttacgaccgc ctggccgaat agctgcctgg cgagcgtttc cttggcgtgc gtgccgaagg
     3121 cctgcttttt ttccggggca tcgattcccg atagcctgat ccgaaactgc cggttctcgg
     3181 atgtcagtac tgtgacggta tcgccatcac ttacgccgac cacccgacct tcaagcgtgg
     3241 ccgctgtagc ataagcgcat accgccagca gcatcagcgc tgcaagcgca atcctgttca
     3301 tcgtttcccg gcctgtgcgt accggtagat ctatagttgt gtgcgcagta tgacactgtc
     3361 ccgaccatcg cgctgtagcc tacggcaggc atgctgctag aatccggggt actttccact
     3421 cctgcaaatc gcgtcccata tgagttccag actgttcacg cccttcgaga tgcggggcgt
     3481 tacgttgccc aatcgcatcg tggtctcccc gatgtgccaa tacatggccg aggagggatc
     3541 ggcgaatgat tggcatctga tgcacctcgg tcaattcgcc atgggcgcgg gaggcctggt
     3601 catcaccgaa gccacgcatg tatcgcccgt cggtcgcatc acgccgcgtt gtctcgggct
     3661 ttatagcgat gacaacgaaa gaaccctcgg acatgtggtg gatttctgcc gcaagtacgg
     3721 cgtggtccgc atgggcattc agctcgcgca cgccggacgc aagggatcga cccatactcc
     3781 attccagggc ggcaaaccgc ttaacagcaa cgaaggcgcg tggactaccg tcgcgccgtc
     3841 cccgttgcca tatgcacccg gttggcacgt gcctgagccg ctgagtgaaa gcggcctcgc
     3901 cgataccaaa caacagtttg tcgaatccgc caggcgtgcg ttgcgcatcg gcttcgatct
     3961 cattgaactg cactccgcgc acggctatct gctgcaccag tttctgtctc cgatatcgaa
     4021 tcagcgcacc gaccgctacg gtggcgctct ggaaaatcgc atgcgttttc cgctcgaagt
     4081 gttcgaagcc gtgcgtgcgg tctggccgca ggaccgtgtg ctcggagtgc gtgtgtcggc
     4141 caccgactgg atcgaaggcg gctggacacc ggaggagacc gtggtatatg cccgggaact
     4201 taaaaatcgt ggctgcgatt tcatcgatgc gagttccggc gcactcgatc cacgccagca
     4261 gattccattt ggacccggct acaacgttcc cttcgccgag aaaatcaaaa aattttccgg
     4321 catcgcgacc atggcggtgg gcttgatcac aaagccggca caggcggaag aaatcatcag
     4381 cagcggcaag gcggatctta ttgcgatcgc gcgcgcggcc atggatgatc cgcgctgggc
     4441 ctggcacgcg gcgcgcgagc tgggtgacga agcggcctat gccccgaact atgtgcgggc
     4501 aagtcccgcc gtgtggaagc cctagccacg caggacggga tcagcaactg ctccacatga
     4561 gtgcgatcgc gcaagtcatg caaggccgcc cgcgcgatct cggcggcttg caggtcaggc
     4621 gcatcctgcc ctatggcggc ggccgcatgg tgggcccttt tatttttctg gatcacatgg
     4681 ggccggtgca gtttgcgcca ggcgccggga tcgatgtgcg accgcacccg catgtttgcc
     4741 tcgctaccgt cacatatctg ttcgaaggcg aaatagagca ccgcgacagt ctcggcgtgg
     4801 tccagaccat tcgtccgggc gaggtcaact ggatgacggc cggcaacggc atagcacact
     4861 cggaacgcac cggggccacg ctgcgcgcac agggccacaa attgcacgga ttgcaatcct
     4921 gggtggcatt gccagtggca gatgaagaaa cgctgcctgc gtttaatcat ttctcgacct
     4981 cgcaattgcc cgtgcgcgaa caggatggcg tagtggtgcg actgattgcc ggagacgctt
     5041 ttggggcgcg ctcccccgta ccggtgttct ccagcatgtt ctatgcagac gtcgtcatgc
     5101 aggccggtgc cagcgttacc gtgccggatg agcacgccca gcgggctgct tacatcgtgg
     5161 aaggagaaat tgagattgag cgtcgcaaat tcggcgaagg ggctttgctg ctgtttgaag
     5221 aaggcgagcc ggcccggctg cgcgcttcgg gtgccagccg ggttgcagtg ctcggcggag
     5281 cgccgcttga tggcgagcgc catatctggt ggaacttcgt ggcaagttcg ccagaacgca
     5341 tagagcgcgc aaagcgggat tgggcgaacg gcatgtttgc ccgcataccg ggtgaaacgg
     5401 agtttattcc gctgccgcct tcttgacccg gccggaccag cggatcgacg cccggacacc
     5461 ggccgtcggc gatgtgcgaa tctttcgacg caacgcgcta tagtatccag gcaggtcaaa
     5521 cctataacgg ggggtacacc atgaaactct atttcgatcc aatcagtgtg aattgccgca
     5581 aggttgtcgc cgggttcgga ctcatgggcg tggattacga atcggcgcat gtcgattact
     5641 tcaaggctga gcaaaaagcg cctgcttacc tgcagatcaa tcccaatggc gccattcccg
     5701 cgctggttga tggcgaaatg cagctatggg aatccaacgc catcctgcag tacgcggcgg
     5761 acaagaatga agctttcggt tattacccga aagacctcaa aatgcgcgcc gatatcaatc
     5821 gctggctgct gtgggaagcg gcccactggt ttccgagtaa ctatgtctac ctcgttgaaa
     5881 acgtcgtcaa gccgctgctg aaggcgcaac ccgacaaggc catcatcgac aaggaagcac
     5941 cgaactggca tcgccttgcg gggatcctgg atgcgcgtct cgccaagcac aaatggctgt
     6001 gcggagacaa ggtgaccctg gctgacatcg ccgtcgctgc accgttgcat ctgcatccct
     6061 atcagaaagt gccgctcgat gcgcacccca acttgaagcg ctggatgacg cagggcatcg
     6121 agcagctgcc atgctggaag gcaacggacg cggcgaagct gctcgggctc gcggcctgac
     6181 gcgaagtcgg cgcgggcgca tcttccgtta tccacaagga catcatcatg cggcgggaca
     6241 agtcggtgcg tgcctccttc aattacacgc gggacgatgg cacggttccc gaagtctatt
     6301 tctacgagcc gcccgcgggc accgtggtgc atccgcccgg cactgatgca cgtgagatgg
     6361 cgataacgga tgggtggtct cgcgccgatg gcttttcact cgaccgggaa gggtttgcgc
     6421 taaaggagtt tcgcaatccg ttcgaccgct tcgacgacga tgaagcggtc cgcagccagt
     6481 tctacgaatc ggttgcggag tttgtgcggt cttcggtcgg cgcgaagcgc gtgatggtgt
     6541 tcgaccacac catccgctcc aaggtcaacg agcagcagca gaccgcggag cacaccacga
     6601 ctcagcgcgc accggtgatg atcgtgcatt gcgattacac cccggtgtct ggtcccttgc
     6661 gcgtgcgcca gctgctgcct ggcgaagccg aggggctgct gaagcgtcgc gtcgcgttct
     6721 ttaatttctg gaagccgctg aagcgccggg tggaagagaa accgctggcg atgtgtgaag
     6781 tgacttcggc cacccaggac gacatgatca aga
//
LOCUS       DATMZQ010000350        11414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4977639, whole genome shotgun sequence.
ACCESSION   DATMZQ010000350 DATMZQ010000000
VERSION     DATMZQ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4977639"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VN496_11195"
     CDS             complement(<1..1319)
                     /locus_tag="VN496_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXF17564.1"
                     /translation="MSVSKHPALITASVGLASFLYSVDWTIAAVALPHMQGTFSATQD
                     QIAWVITSYIVASALSIPAAGWLSLRFGRKRVFMWAVIVFLVASVACGAADSLAVEVF
                     SRIVQGLGGAFLIPLSHAIILDTYPPHEQGKAMALWGMASVMGSFIGPTVGGYVTEYM
                     SWRYIFYINIPFGILALAGAAVFLPETKVDPGRRLDWFGFLSLSLGIGSLQLMLDRGG
                     QLDWFESWEIITESCLAVLGLYMFNVHCLTAKQPFLDPRLLAQRNFFLGLVFAFIYGF
                     IATPPMVLMPAFLDHVRGYPIDTIGLLQAPRGFGLFAAMMIGARITGRIDPRVPIAFG
                     LLCMAFSSWEMASWTADVGVWPLVWTNFMQGIGGGIILVPIQVIAFPALEPHRRTEAT
                     AVYNLVRSLGASIGVSGALALLVRTSSVMHSQLAEHATHFNRALQAQG"
     gene            complement(1326..2453)
                     /locus_tag="VN496_11200"
     CDS             complement(1326..2453)
                     /locus_tag="VN496_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXF17565.1"
                     /translation="MSETAQKAVIVVHRAVRIALLVIVPLLAVATGLYLFATGGQEVE
                     TDNAYVKANIVPISAAVTGRVIEVFAHDNERVEAGAVLFRMDPEPYLIAVAGARAQLE
                     VVKTDVQSLRAEYRATLQEAAEARSRIEFLAKQMERQERLKEKGMTRGDTYDEARHNL
                     EAAKRRLDSIREKTNRVVAELSGSPNTPVERLPRYAEAQSAYDAAMLDLARAQVKAPF
                     AGVVSNMRLQVGEYVEKGAPMFSLIETGALWIEANYKETQLTHMTVGQPAKVVADAYP
                     DHEWPASVEAIASATGAEFAVLPPQNATGNWVKVVQRIPVRIKVEQPSGHPQLRAGMT
                     VTVTVDTGRPRGLPRTVQKMVDNGWLPHFLVPASALASTRR"
     gene            complement(2455..2931)
                     /locus_tag="VN496_11205"
     CDS             complement(2455..2931)
                     /locus_tag="VN496_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXF17566.1"
                     /translation="MIREDLSRNFGFILNDVARLMRTTFDRRVKSLGLTRSQWWVLNH
                     LFRNNGVTQSELADILEVEKATLGRLLDRMEQKGWVRREGHAGDRRAKRVFLTKEVEP
                     AIKTMRAAAAELRRDALSGLSASQQNGFVDALLAIKANLSRPDNGANGNGVKNRKR"
     gene            3063..4280
                     /locus_tag="VN496_11210"
     CDS             3063..4280
                     /locus_tag="VN496_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXF17567.1"
                     /translation="MKITAVETLNLAEFPNVLWVQVHTDEGAVGLGETFYAVAPVAEH
                     IHQTCAAYLLGKNPLEIDKHSRQFLNTYLGFNSVGVEMRAASAIDIALWDLFGQVTGQ
                     PIYQLLGGASRDHVRVYNTCAGYQYVRAKPTQGTENFGLPAKVDKKNRPYEDLMAFKT
                     DAGELAKSLLEMGISGMKIWPFDEAAEASNGSYISNADLKKAMRPFEQIRKAVGDKMD
                     IHVEFHSMWNLPAAIRIAEALEQFDPFWYEDPIKMNNLDAVADYAHRTNVWVTASETL
                     GTRWGFRELFEKQAVSVCMLDVGWTGGLSESKKIATMAEAYQLPVAPHDCTGPILLTA
                     SVHLSMNCPNTLVQEMVRAFYYDWYGQLVTELPPVKDGYITAPKGPGLGTKLQPSVLK
                     RKDLTRRISKLGK"
     gene            4321..4635
                     /locus_tag="VN496_11215"
     CDS             4321..4635
                     /locus_tag="VN496_11215"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXF17568.1"
                     /translation="MNTDPRVLVLAPGDNVAIAKSDIPAGTTLQVMDVTVTVKATMET
                     GHKFAFKRVAKGEGITKYGAPIGVATQDIAPGDSMHIHNVTSDYIPTYTLDEGHQFFK
                     EH"
     gene            4638..5834
                     /locus_tag="VN496_11220"
     CDS             4638..5834
                     /locus_tag="VN496_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXF17569.1"
                     /translation="MINAYLRADGRKGIRNTLVVAYLVECAHHVSREIYYPFREQGVH
                     LIGFPGCFPNVYSHKMMERLCTHPNVGGVLLVSLGCEGFNKGKLQETVRKSGRPVKLI
                     EIQSTGGTRKTIAEGKAWIEQTLQQLASTPRANMALDELIVGTICGGSDATSGLTANP
                     AMGRAFDFLVEQNATCIFEETGELIGMEHWMAKRAENPVLGDEIVKTVAKAAKYYATY
                     GHASIGPGNADGGLTTIEEKSLGAYAKSGQSVISGIIKPGDVPIRGGLYLLDVVPDGE
                     PLFGFPNISDNAEIAELMACGSHLSLFSTGRGSVVGSAIAPVIKVCANPHTYKRMSDD
                     MDVDAGRILENRATLDEVGREIYDLIIRVANGEQTKSEELGHQEFILTYKSFEPIGPA
                     CFPSAA"
     gene            5863..6258
                     /locus_tag="VN496_11225"
     CDS             5863..6258
                     /locus_tag="VN496_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXF17570.1"
                     /translation="MSIVPLYASILALLFMVLSIRTVQMRRRFKIGIGDAGNNAMLRA
                     MRVHSNFAEYVPLSLLLIFFVEMAGASLLLVHGLAWCLLLGRASHAFGVSQIRENYRF
                     RVFGITMTFAVLIAASFYLLYSYASRSVA"
     gene            complement(6352..6558)
                     /locus_tag="VN496_11230"
     CDS             complement(6352..6558)
                     /locus_tag="VN496_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17571.1"
                     /translation="MKKRTLNDGPERLRKLWSAYEYPGRLVRRDIVRLGRILRLAYVA
                     SIGRKAYVPDPDESEQYLWAERRE"
     gene            6801..7151
                     /locus_tag="VN496_11235"
     CDS             6801..7151
                     /locus_tag="VN496_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HXF17572.1"
                     /translation="MGWLITKYFLTAAVVVVVSEVAKRSDKLGGFVAALPLVTVIALI
                     WLYVERQPTEKIANHAWYTFWYVVPTLPMFLAFPVLLPRLGFWPSLLACVVITTVCFG
                     LFALTLRRFGINLL"
     gene            7495..8112
                     /locus_tag="VN496_11240"
     CDS             7495..8112
                     /locus_tag="VN496_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17573.1"
                     /translation="MAVSIALGGCAAATNKNEPDLAAQLHDAEQLYEEQGNAVRAEML
                     IWETMEAYRSKGDQLGLAEAYRAYGFFLRAQAVEGQWIEHYKTAGFLDPSVTFDIRYE
                     KSIQYLERARTIYTAYGRYDALTNVNLNIGFTYEVLGNPEAACWAFDNSISSNREGLE
                     RHAKLQIRLPKGYANYEDFLEPHRKRAGCEALKLHRRKQPSVMTA"
     gene            8303..9169
                     /locus_tag="VN496_11245"
     CDS             8303..9169
                     /locus_tag="VN496_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17574.1"
                     /translation="MATIVHEGPGNERRTSPRRNGDHTVLHRSANLIDVQGMSVSWGG
                     VWGGVLIGMGLLMLLTALGLALGVSAVDPNSDTQSLGTPAAIWAAISLLLALYIGGMA
                     STRIGAIFDKTTGMFEGTLVWVLSLLLIVYLAGSGIGLVAGGAFKLVGGATQAIGSMM
                     TGGTPQISEANMNQIVQHLREPQTSRMLASATGMPEEKVRTNLAQIADKADAAKDNPA
                     QAATEVRQSVQDMIQKARTDGSLAQAAEKAKGAASKTAWITFAALVLSLLAAVLGAMT
                     GRRAAALTVGRE"
     gene            9364..10458
                     /locus_tag="VN496_11250"
     CDS             9364..10458
                     /locus_tag="VN496_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17575.1"
                     /translation="MRRAHCAATLTLLFSLAMTQVQAADTPEFRVDPFWPKPLPNNWI
                     LGQIGGIAIDRHDHVWVYQRPRTLTDDEKGAAVDPPTSKCCRPAPPVLEFDPAGNLLQ
                     AWGGEGTGYDWPRSEHGIFIDASEHVWLAGNDKENDAQVLEFTREGKFVKQIGEARHT
                     EGSNSTRYLGRPALAIVDDAAHEVYVADGYGNKRVVVFDSRTGEYRRHWGAYGAKPDD
                     ADPGKYDPGAPLANQFRNPVHCVRISHDGLVYVCDRVNDRYQVFRKDGSFVAEFRLEP
                     NTRFVGSVWDIGFSPDAGQKYLFICDGTNNEIHILLRETGKEVGAFGRQGRNAGEFHW
                     VHTMAIDSHGNIFTGDVDTGKRVQRFERIQ"
     gene            10469..>11414
                     /locus_tag="VN496_11255"
     CDS             10469..>11414
                     /locus_tag="VN496_11255"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HXF17576.1"
                     /translation="MTTRRKPPFRADHVGSFLRPRALLEAREQNKNGSLSRAELRKIE
                     DAAIRDIVRFQEVLGLKGITDGEFRRTYFHVDFLEQLQGVETRGGIPVSFRSAGGNVD
                     FSPPVMQVTGPLRHVRPIQLADFQFLKSVTEKMPKVTIPSPTMLHFRGGREAISKQSY
                     PELEPAFYDDVARCYRAELQSLAEAGCRYVQMDDTNLAYLCDPKMRNGARQRGDDPDE
                     LPRRYARLINAAIRERPADMTVCVHLCRGNFRSAWVAEGGYEPVAEVLFNELAVDGYF
                     LEYDDARSGTFAPLRFVPKGKTVVLGLVSTKVGDLESKD"
BASE COUNT         2418 a         3334 c         3357 g         2305 t
ORIGIN      
        1 ccttgcgcct gcaaggcgcg attgaaatgc gtcgcgtgct ccgccagctg ggaatgcatc
       61 acgctgctgg tccggaccaa caaggccagc gcgcccgaca caccgatact tgctccaagg
      121 ctgcgcacga ggttgtagac ggcggtggcc tccgtgcgcc ggtgcggttc gagtgccgga
      181 aaagcgatga cctgaatcgg tacgagaatg atgcccccgc cgatgccttg catgaaattg
      241 gtccacacca agggccatac accgacgtcg gctgtccagc ttgccatttc ccagcttgaa
      301 aacgccatgc acaacaatcc gaatgcaatc ggcacgcgcg gatcgatgcg cccggtaatg
      361 cgcgcgccaa tcatcatggc agcgaacagg ccaaagccgc gcggcgcctg cagcaggcca
      421 atggtatcga tcgggtagcc gcgcacgtga tccagaaacg ccggcatcag gaccattggc
      481 ggcgtggcga tgaaaccgta gatgaacgcg aacacgagtc cgagaaaaaa attgcgctgt
      541 gccagcagtc gcggatcgag gaaaggctgc tttgccgtca ggcaatgcac gttgaacatg
      601 tagagaccca gcaccgcgag acaactttcg gtgatgattt cccaggactc gaaccagtcc
      661 agctggccgc cgcgatcgag catgagctgc agtgagccga tgcccagtga caggctcaga
      721 aagccaaacc agtccagccg ccgaccggga tcgaccttgg tctcgggcag aaaaaccgcc
      781 gcaccggcca gcgccagaat gccgaacgga atgttgatgt agaaaatgta acgccagctc
      841 atgtactcgg taacgtagcc gcccaccgtg ggcccgatga aagagcccat taccgatgcc
      901 atgccccaaa gtgccatcgc cttgccctgt tcgtgcggcg gataggtgtc gagaatgatc
      961 gcgtgcgata gcggaatcag aaatgccccg cccagcccct gcacaatgcg cgaaaagact
     1021 tccactgcga gggagtccgc ggcgccacag gcgaccgatg cgaccaggaa tacgatcacc
     1081 gcccacatga aaacccgttt tcggccgaag cgcaggctca gccagcccgc agcaggaatg
     1141 gaaagcgcgg atgccacgat atatgacgtg atgacccagg cgatctgatc ctgcgtggcg
     1201 gagaacgtac cctgcatatg cggcagtgcc accgcggcga tggtccagtc caccgaatac
     1261 aaaaagctcg ccagcccgac cgaagcagta atcagggcgg gatgcttcga tacgctcacg
     1321 atcgattagc gacgcgtgct ggccagtgcg gaagccggca ccaggaagtg cggcagccag
     1381 ccgttgtcca ccattttctg cacggtgcgc ggcaggcccc ggggccggcc cgtgtcgacc
     1441 gtcacggtca ccgtcatgcc agcgcgaagc tgaggatgac cggacggctg ttcgaccttg
     1501 atgcgcaccg gtatgcgctg cacgaccttg acccagttgc cggtcgcgtt ttgtggcggc
     1561 aaaacggcaa attcagcacc cgtggccgat gcgattgctt ctacgctcgc cggccattca
     1621 tgatccgggt aggcatcggc taccactttt gccggctggc cgacggtcat atgcgtcagt
     1681 tgcgtttcct tgtaattggc ttcaatccac agcgctccgg tttcgatcaa gctgaacatc
     1741 ggcgcgccct tctcgacgta ctcgccgacc tgcagcctca tgttgctcac cacgccggcg
     1801 aacggcgctt tgacctgcgc gcgcgccagg tcaagcatcg ccgcgtcgta agcggattgc
     1861 gcctcggcat agcgcggcag gcgttcgacc ggcgtattgg gactgccgga taattcagcg
     1921 actacgcggt tggttttttc gcggatgctg tcgagcctgc gctttgcagc ttcgagattg
     1981 tggcgcgcct catcgtaggt atcgccgcgc gtcatgccct tttctttcag gcgctcctgg
     2041 cgttccatct gtttggcgag gaattcgatg cggctgcggg cctcggccgc ttcctgcaac
     2101 gtcgcacggt attcggcacg cagggattga acgtcggtct ttacgacctc caattgggcg
     2161 cgtgcgccgg caaccgcgat cagatagggt tccgggtcca tgcggaacag gaccgcgccc
     2221 gcctccaccc tctcgttgtc atgcgcgaag acttcgatga cccggccggt cacggcggcg
     2281 ctgatcggga cgatgtttgc cttgacatag gcgttatcag tttcgacttc ctggccaccg
     2341 gtggcgaaaa gatacagccc ggttgccacg gccagcagtg gcacgataac cagcagcgca
     2401 atgcgcaccg cacgatgcac gacgataacc gccttctggg ctgtctccga catatcagcg
     2461 cttgcgattt ttcacgccgt tgccattggc gccgttgtcc ggtctggaca aattcgcctt
     2521 gatggcgagc agcgcatcga cgaagccgtt ttgctgcgac gctgataatc cggacagcgc
     2581 gtcgcgccgc aattccgctg ccgcagcgcg catggttttg attgcgggct cgacttcctt
     2641 ggtcaggaac acgcgcttgg cgcgccggtc accggcatgg ccctcgcgcc ggacccagcc
     2701 tttttgttcc atgcgatcca gcaggcggcc caaggtcgcc ttctcgactt ccagaatgtc
     2761 cgccaattcc gactgcgtaa cgccgttatt gcgaaacaga tgattcagga cccaccactg
     2821 cgagcgtgtc aggccgagcg atttcacacg ccggtcgaac gtggtgcgca tcagccgggc
     2881 cacatcgttc aggatgaagc cgaaattgcg ggacaggtct tcacgaatca tgaccttgga
     2941 ttctaccgaa aaactggtcg cctagcttac tatatgccag gttatcatcg cgggaccggc
     3001 cggccgtcgc cttggcggac ccggctgcta gaattgcgcc atcaaagcgg aggaccgtaa
     3061 taatgaaaat aacagccgtc gaaacgctca acctggcgga atttccgaat gtactgtggg
     3121 tgcaggtgca taccgacgag ggcgccgtcg gcctcggaga aaccttttat gcggtggcgc
     3181 cggtggccga acatatccac cagacttgcg ccgcgtacct gctgggcaag aatccgctcg
     3241 aaatcgacaa gcacagccgg cagtttctga atacctacct cgggttcaat agcgtgggcg
     3301 tggagatgcg cgccgcatcc gcgatcgaca tcgcgctatg ggacctgttt ggccaggtca
     3361 ccggccagcc catctaccag ctgctgggcg gcgcctcgcg cgatcacgtg cgcgtgtaca
     3421 acacctgcgc gggctaccag tacgttcgag cgaaaccgac gcagggcacc gagaacttcg
     3481 gccttccggc gaaagtcgac aagaagaacc ggccgtatga ggatctcatg gcgttcaaga
     3541 ccgacgccgg cgagctcgcg aagtcgctgc tggagatggg catcagcggc atgaaaatct
     3601 ggccgtttga cgaagccgcg gaagcgtcca atggcagcta tatttcaaat gcggatctca
     3661 agaaagcaat gcggccgttc gagcagatcc gcaaggcagt cggcgacaag atggatatcc
     3721 acgtcgagtt tcactcgatg tggaacctgc ctgctgcaat ccggattgcg gaagcactag
     3781 aacagttcga cccgttctgg tacgaggatc cgatcaagat gaacaatctc gatgcagttg
     3841 ccgactacgc gcaccggacc aacgtctggg tcacggcctc cgagacgctg gggacgcgtt
     3901 ggggcttccg cgaactgttt gaaaagcagg ccgtgtcggt ttgcatgctt gatgtcggct
     3961 ggaccggtgg cttgtcggag tccaagaaaa tcgccacgat ggcagaagct taccagctgc
     4021 ccgtggcgcc gcatgactgc accggtccca tcctgttgac ggcctcggtg cacctgtcga
     4081 tgaattgccc gaacacgctg gtgcaggaga tggtgcgcgc gttctattat gactggtacg
     4141 gtcagctcgt cactgaactg ccgccggtga aggacggcta tatcactgct ccaaaaggcc
     4201 cggggctcgg gacgaagctg cagccatccg tgctgaagcg caaagacctg acccgcagaa
     4261 tttcaaagct gggaaaatga gcgaatcgcg aaatgccacg aaccatatat gaacacgacg
     4321 atgaacactg atccgcgcgt acttgtgctg gcccctggag acaatgtagc gatcgccaaa
     4381 tccgacatcc cggctggtac gacgctgcag gtcatggacg ttacggtgac ggtgaaggca
     4441 acaatggaaa ccggccataa gtttgcgttc aagagagtcg ccaagggtga aggcattacc
     4501 aaatatggcg cgccaatcgg cgtggcaacc caagacatcg cgccgggcga ttccatgcac
     4561 atccacaatg tcaccagcga ctacattcca acctacaccc tcgatgaggg ccaccagttc
     4621 ttcaaggagc attgatcatg attaatgcct atcttcgtgc cgacggccgc aagggcatcc
     4681 gcaacacgct cgtggttgcg tacctggtcg aatgcgcgca ccacgtctcg cgggaaattt
     4741 actatccgtt tcgcgaacaa ggcgtgcatc tgattggctt tccgggctgc ttcccgaacg
     4801 tgtattcgca caagatgatg gagcgccttt gcacgcaccc gaatgtgggc ggcgtactgc
     4861 tggtgtccct gggttgcgaa ggcttcaaca agggaaagct gcaggaaacc gtgcgcaaat
     4921 ccggtcgtcc ggtgaagttg atcgaaatcc agagcaccgg cggcacgcgc aagaccatcg
     4981 ccgaaggcaa ggcgtggatc gaacaaacct tgcagcagct ggcaagcacg ccacgcgcca
     5041 acatggcact ggacgaactc atcgtcggta cgatttgcgg tggatccgat gcgaccagcg
     5101 gcctgaccgc caatccggca atgggtcgcg cgttcgactt cctggtggag cagaacgcga
     5161 cctgtatttt tgaggaaacc ggggaactga tcggcatgga gcactggatg gcgaagcgag
     5221 ccgaaaatcc agtgctcggt gacgaaatcg tcaagaccgt tgcgaaggcc gcgaagtact
     5281 acgcgaccta tggccatgcc agcattgggc cgggcaatgc cgatggcggg ctgaccacca
     5341 tcgaggaaaa atcgctcggc gcctatgcca agtcaggcca gtcggtcatc tcgggaatca
     5401 tcaagccggg tgacgtgccg atccgcggcg gattgtattt gctggatgtc gtgccggacg
     5461 gcgaaccgct gttcggattc ccgaacatca gcgacaacgc cgagattgcc gaactgatgg
     5521 cgtgcggcag ccatttgagt cttttctcca ccggccgcgg ctccgtagtg gggtccgcta
     5581 tcgccccggt catcaaggtc tgcgccaatc cgcatacgta caaacgcatg tcagatgata
     5641 tggatgtcga cgccggccgc atcctcgaaa accgcgccac gctcgatgaa gtcggccgcg
     5701 agatttacga cctgatcatc cgggtcgcta acggcgagca gaccaaatca gaagaactcg
     5761 gacatcagga atttattctg acctacaaaa gcttcgaacc cattggaccg gcctgctttc
     5821 cttcggccgc gtaaccgtag caactggtgg agtgtgtaga cgatgagtat cgtccccctg
     5881 tatgcatcga tactggccct gctgttcatg gtgctcagca ttcgaacggt tcaaatgcgt
     5941 cgtcgcttca agatcgggat cggcgacgcc ggcaataacg caatgctgcg tgccatgcgg
     6001 gtgcattcca atttcgcgga atatgtaccc ttgagtctgc tgctgatctt ttttgtagag
     6061 atggcgggcg ccagcctgct gctcgtgcac ggactggcgt ggtgtctcct tttgggccgg
     6121 gcatcgcacg cgttcggcgt cagccagatc agggaaaact atcgctttcg tgtatttggt
     6181 ataacgatga cgttcgctgt cctgatcgcc gcgtcttttt acctgctgta ttcctatgcg
     6241 agcaggagcg tagcgtagcg tcggcatcaa aacattcatt cggatgacct atctagcgtt
     6301 gcacgggtcc agctcaatgc cgcagtcgtt tttcgccgag ccgtttgctt tttactcgcg
     6361 ccgttcggcc cacaaatatt gttcgctttc gtccggatcc gggacatagg ccttgcggcc
     6421 gatcgacgca acataagcaa gtctgagtat tcttccgagg cgaacgatgt cgcgacgcac
     6481 cagccggccc ggatattcat acgccgacca aagctttctc aagcgctcgg gaccgtcatt
     6541 cagtgtgcgt tttttcatgg tgacactccg ctcaatttgg tgttgcctgc aacttccata
     6601 tcgcatgccg tgtgccaatc gcgggtgtcg aaaaagtggc atcgccttgc gctcgaaatc
     6661 catccattcc ggtagatata caggtgccgg aaaaccggca aatggacctg acgaactatc
     6721 aacacgcggg ttggcgcaga atgtcaatgt tcgccggcct acattgcagt tattcgcacc
     6781 ggacatgatt ggatgaacag gtgggttggc tcattaccaa gtattttctg actgcggccg
     6841 tcgtggtcgt ggtttcagag gttgccaagc gcagcgacaa actgggcggc tttgtcgccg
     6901 cactgccgct ggtaaccgtc atcgcgctca tttggctgta cgtggaaagg caaccgacgg
     6961 agaagattgc caatcacgcc tggtatacct tctggtatgt ggtgccgacc ttgccgatgt
     7021 ttcttgcctt tcccgtattg ctgccgcgtt tggggttctg gccctcgttg ttggcctgcg
     7081 ttgtaatcac caccgtatgt tttggcctat ttgcgcttac gctgcgccgc ttcggcatta
     7141 acttgctgta gctacccccg gcaacatcgc gacacagact gaacgccttc ctggcgtttc
     7201 gcaaacggcg cgcctttgct tgtcgatgaa acgcgtccta ctccatacct aaaccctgtc
     7261 ggcgacacag caaaagccgt aagcaacgaa gcggcttgta acttttaccg actgaattca
     7321 aatcagttct ccgaacccgc gaaagtgatt tgcaaacgcg cgcggactcc gtaatatcgg
     7381 gcggcaatat ttcttccgca aaccgcggtt tggcggcacg gatatcagta cttctttaag
     7441 ttcagtaaag gagggacgac cttgcgctgt tttgaaatct tttgcgtttt tgttatggct
     7501 gtatcgattg cgctaggcgg ctgcgcggca gccaccaaca agaatgaacc ggacttggcc
     7561 gcgcaactgc acgatgccga acagctctat gaagaacagg gtaacgcggt gagggccgaa
     7621 atgctgatct gggaaaccat ggaggcctat cgaagcaaag gcgatcaatt gggcctggcg
     7681 gaggcatatc gagcttacgg cttttttctg cgcgcacagg ctgtcgaagg ccagtggatc
     7741 gagcactaca agacggcggg ctttctggac ccatccgtca cattcgatat ccgctatgag
     7801 aaatccatcc agtatctgga acgagcacgc accatttata ccgcctacgg gcgctatgac
     7861 gcgttgacca acgttaacct caatattggc tttacctatg aggtcttagg caacccggaa
     7921 gccgcttgct gggcattcga caacagcatc agcagtaatc gcgaaggtct cgagcggcac
     7981 gctaaacttc agataaggct ccccaagggt tacgcaaact acgaagactt tcttgagccc
     8041 caccgcaaac gcgccggatg cgaggcgttg aaactgcacc gcaggaaaca gcccagcgtt
     8101 atgaccgcgt gatcgaaatc accttcgctt gattatcggg tccacgcaat atttgccttc
     8161 atccttgtaa ttttttactc cttccagact acgttcggcg taacacgtgt tgcgacaccc
     8221 gcgcgttagc catgcgcacc tcaaatgata cgtaggcgcg aaccttgcat cggtcgccct
     8281 atattcgtct tgcgaggcaa tcatggctac tatcgtgcac gaaggacccg gtaacgaacg
     8341 tcgaacttcc ccacggcgca atggagacca caccgttttg caccggtccg ctaatctgat
     8401 cgacgtccag ggcatgagcg tttcctgggg cggcgtctgg ggtggggtac tgatcggaat
     8461 gggactgctg atgctgctca ccgccctggg actggcgctg ggcgtctccg cagtggatcc
     8521 caactcggat acgcaatcac tcggaacacc ggccgcaata tgggcggcca tatcgctgct
     8581 gctggcgctg tacattggcg gcatggcttc cacacgcatt ggcgcgatat tcgacaaaac
     8641 caccggaatg tttgagggca cactggtttg ggtgctcagc ctgctgctga ttgtctatct
     8701 ggcggggagc ggcattgggc tggttgctgg tggcgcattc aagctggttg gcggcgcgac
     8761 gcaggcaatc ggttcaatga tgaccggagg aacgcctcaa atttccgagg ccaacatgaa
     8821 ccagattgtt cagcatttgc gtgagccgca aacctcgcgc atgctggcct cggctaccgg
     8881 catgcccgag gagaaggtcc gcacgaacct ggcccagatc gccgacaagg cggacgccgc
     8941 gaaggacaat ccggcgcagg ccgccacgga ggttcgccaa agcgtgcagg acatgattca
     9001 aaaggcacgc actgatggat cgctcgctca ggcagccgaa aaggccaagg gcgctgccag
     9061 caagacggca tggattacgt tcgccgcgct ggtgttgtct cttctggccg cagtgctggg
     9121 ggccatgacg ggacgccggg cggctgcgct gacggttggc cgcgaatagc tgcgcacatc
     9181 ccttgaaaca gtagagtaat agcgtttcac cgaagggcgg gcctgatggc ccgccctttt
     9241 tattggatgg tagactccgt cgcgtcactc cctcgttcga ctggcaagcg ttccaggctt
     9301 acgtcagctg tgcgcggctt tcacgccgta cgaacgataa cccgatcgcc ttacggaggt
     9361 gcaatgagac gagctcactg cgcagcaaca ctgacgcttc tgttcagctt ggccatgaca
     9421 caggtgcaag cggctgatac gcccgaattc cgcgtcgatc cattctggcc aaagccgctg
     9481 ccgaacaact ggattctggg acagataggc ggcatcgcaa tcgaccgtca cgatcatgtc
     9541 tgggtgtacc agcggccgcg cacgctgacc gatgacgaaa aaggcgccgc agttgatccg
     9601 ccgacttcca agtgctgcag gccggctccg ccggtgctgg aattcgatcc ggccggcaat
     9661 ctgcttcagg cgtggggagg cgaaggcacg gggtacgatt ggccgcgcag tgagcatggg
     9721 atctttatcg atgccagcga acacgtctgg ctcgccggca acgataagga aaacgacgcc
     9781 caggtgctgg agttcacgcg cgaaggaaaa ttcgtgaagc aaatcggcga agcgcgccat
     9841 accgaaggca gcaacagcac gcgctatcta ggccgccccg cattggcgat cgtggatgat
     9901 gcggcccacg aggtttatgt cgcggacggt tatggcaaca agcgcgtcgt ggtgttcgac
     9961 tcgcgcaccg gcgaataccg gcgccattgg ggcgcctatg gtgccaagcc cgatgacgca
    10021 gatcccggca agtacgaccc gggtgcaccg ctggcgaatc agttccgtaa tcctgtgcat
    10081 tgcgtgcgca tttcgcacga cgggctcgtg tatgtctgcg atcgagtcaa cgaccgttac
    10141 caggtattcc gcaaggacgg cagttttgtt gcggaattcc ggctcgaacc gaatacgcgt
    10201 tttgtgggct ccgtctggga tattggcttc tcgccggatg cgggacagaa atacctgttc
    10261 atctgtgacg gtaccaataa cgaaatccat attctgttgc gcgaaactgg caaggaggtc
    10321 ggggcgttcg ggcgccaggg tcgcaacgcc ggtgaattcc actgggtgca taccatggcc
    10381 atcgattcgc acggcaacat ctttaccgga gacgtcgata ccggaaaacg cgtacagcgt
    10441 ttcgaacgga ttcagtagcc gatcaaccat gacgacacgc cgaaagccgc cgtttcgcgc
    10501 cgaccacgtc ggcagtttcc tgcgtcccag ggcgcttctc gaggctagag aacaaaacaa
    10561 aaacggcagt ctgtcgcgtg ctgaattgcg caagatcgag gatgccgcga tccgtgacat
    10621 cgtccgcttc caggaagtcc tgggactcaa aggcattacc gacggcgaat tccggcgcac
    10681 ctattttcat gtcgacttcc tcgagcaact gcagggcgtg gaaaccaggg gtggcatccc
    10741 ggtgagcttc cgcagtgctg gcgggaacgt tgatttttct cccccggtca tgcaagtgac
    10801 tggcccgctg cgccatgtgc gcccaattca gctggctgat ttccagttcc tgaagtctgt
    10861 cactgagaaa atgccgaaag tcaccattcc ttcgccaacc atgctgcatt tccgcggtgg
    10921 gcgagaagct atcagcaagc agtcttatcc ggaactggag cctgctttct acgatgacgt
    10981 ggcgcgctgc tatcgcgcgg aactgcaatc actcgctgag gccggttgcc gctatgtgca
    11041 gatggacgat acaaatctcg cttacctgtg cgacccgaaa atgcgcaatg gcgcgcgcca
    11101 gcgcggcgac gatcctgatg aactgcctcg ccgctatgcg cgcctgatta atgcggcaat
    11161 cagagagcgc cccgccgaca tgacggtatg cgtgcatttg tgccgcggca atttccgcag
    11221 tgcctgggtc gcagagggtg gttacgaacc agtggcggag gtattgttca atgaactcgc
    11281 cgtggacgga tatttcctgg aatacgacga tgcgcgttca gggacctttg ctccattgcg
    11341 attcgtgccg aaaggcaaga ccgtcgtgct cgggctggtg agcaccaaag tgggggacct
    11401 ggaatcgaag gatg
//
LOCUS       DATMZQ010000351        20534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1525947, whole genome shotgun sequence.
ACCESSION   DATMZQ010000351 DATMZQ010000000
VERSION     DATMZQ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525947"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VN496_11260"
     CDS             <1..1401
                     /locus_tag="VN496_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXF17577.1"
                     /translation="LARFEVPEEFKPLLPHLLYQPDKGCIEYKALEQAAALAHLTIPR
                     LLERSGAFASPRDYHFGRFLFEYFPGGTSFDPGLVAMPPGALPLSPAECFSIDDATTT
                     EIDDAFSVTKLANGNWLVGVHIAAPALGIAPGSPLDREAAKRLSTVYFPGDKITMLPE
                     NAIAQYTLSEGGDCPALSMYIEVTPQFEVVAHRSVVERVHIAANLRFDELETEFNEQQ
                     IASGQISHRFGEQLMVLWHLAARLERQRGKDEVANARSDYNFHVENDRISIVARRRGS
                     PVDKVVSELMILVNTQWGGELAQNGIPALYRAQSGGKVKMSSVPAPHQGLGVPQYIWA
                     SSPLRRYADLVNQRQLLSLISNEAVPYPAGDERLLVILRDFELAYDAYAEFQRGMERY
                     WCLRWLIQEDISLTAAEVLREELVRIERIPLVVRVPSMPATSPGTRVELVLSNIDLLD
                     LTVHCEFKQTLEALSR"
     gene            1456..2382
                     /locus_tag="VN496_11265"
     CDS             1456..2382
                     /locus_tag="VN496_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXF17578.1"
                     /translation="MPDTSISALPAPGSLAAGLAISPPPASGDMLPITLAISVVLHAV
                     VLLITFAPPDFDSSKFAPHLDVVLVNSKSVSRPLKADALAQANLDGGGNTDEDRRAKT
                     HLPKLNDVEPARDVQLSSRQVRRLEQEAQRLLLLSKGTHATSDSTQPAPELPREALLE
                     DPAAEQQRLRIAQLEAQIAKDWDEYQKMPRRKFIGARTEGVVYAEYVDRWRQRVEQVG
                     TQHFPDEARQRKLYGSLVMTVHIRADGSVEKVEIDRSSGYRLLDSAALQAVDLAGPFP
                     PFPAAVRKEWDILSISRTFSYTRSDLEVLVNP"
     gene            2379..3206
                     /gene="aroE"
                     /locus_tag="VN496_11270"
     CDS             2379..3206
                     /gene="aroE"
                     /locus_tag="VN496_11270"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXF17579.1"
                     /translation="MTDRYAVVGNPVAHSKSPEIHAAFARQTGQDIEYSKLLAPPGGF
                     RSTIEAFRADGGRGVNVTVPFKLEAFALADDLSQFARNAQAANLLQFDGGRIRAHNTD
                     GIGLLRDIVHNLGFGIADKRVLLMGAGGAARGVLTPLLEYGPRILTIANRTIDKALRL
                     AETFRHLPAASSSVLSGIGYGELVGHHFDLVINATSSSLNGELPPLPADLFAPDALAY
                     DMMYGKTLTPFLAFSAARGAKYSDGLGMLVEQAAESFLHWRGVRPQTAPVIELLRNS"
     gene            3203..3907
                     /gene="mtgA"
                     /locus_tag="VN496_11275"
     CDS             3203..3907
                     /gene="mtgA"
                     /locus_tag="VN496_11275"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXF17580.1"
                     /translation="MSAMRRAWRWPARIMLVLIAAVVVYQFWIMGSVVWWVWFNPSTS
                     AFMADRLEALQLHDPDAVLHHQWVPYEHISINLKRALIAAEDAKFLDHDGFDWEGIQK
                     AYEKNLKKGRVVAGGSTISQQLAKNLFLSGRRTPWRKAEEALITMMLENMMSKRRIFE
                     IYLNIIEWGNGVFGAQAAAHYYYGTSASNLTAEQAARLAAMVPKPRYYDKVRDTPLLE
                     RQTAIILERMWQVAVP"
     gene            3932..4717
                     /gene="lgt"
                     /locus_tag="VN496_11280"
     CDS             3932..4717
                     /gene="lgt"
                     /locus_tag="VN496_11280"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXF17581.1"
                     /translation="MLIHPQFDPVLVHLGPLAIRWYGLMYLLAFLLVLVLGRYRIRTQ
                     PWRDVSVRDLDDMLFMGVVGVVVGGRLGYILFYKLADYMKEPLHIFYVWEGGMSFHGG
                     FLGVILAMLWFARSRGKRWLALTDFIAPLVPLGLGAGRLGNFINAELWGRPTDVPWAM
                     IFPNIDNVPRHPSQLYEFGLEGLVLFGLLWWFSSKPRPTGAASGLFLLGYGLLRFVVE
                     YSREPDGFLGLLALGFSMGQWLSLPMAITGMALLAWAYRKKTS"
     gene            complement(4710..5474)
                     /locus_tag="VN496_11285"
     CDS             complement(4710..5474)
                     /locus_tag="VN496_11285"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HXF17582.1"
                     /translation="MKISPPLGYEQITPLLKSHKVLLPRAEETPAVFHRLHVMPLSLA
                     EFEPACRDYPIVFLTNDGGKNFSAVLVLGMQVNQNLFILTDGTWDRRVYLPAYVRRYP
                     FCMSRVSVAGEPQKERIVCIEKSALQESGEPLYDTRGEALAQWILLEKLIFDFEQDLV
                     RSESLCQLLVEMKLLEPFTMKAEVDGFTMQLEGMHRIARAALEGLPGDQLRRLMSAGA
                     MEKIYSQLLSLDNFRRLLNRRSFFAVKRPEKRRELN"
     gene            5553..5628
                     /locus_tag="VN496_11290"
     tRNA            5553..5628
                     /locus_tag="VN496_11290"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5586..5588,aa:Ala,seq:cgc)
     gene            complement(5647..6489)
                     /locus_tag="VN496_11295"
     CDS             complement(5647..6489)
                     /locus_tag="VN496_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HXF17583.1"
                     /translation="MREPARWPIHGVDFTSRPRRKKPITVASGSRTSRTFRLDAVNEI
                     IDWPGFECWLRRPGPWIAGFDFPFGLPREAVDDLGLPHSYPQLVDHCRAQGREAFRAL
                     LDKYRESRPVGKRYAHRAADHPAGSHSPLKLVNPPVALMFLEGVPRLMDAGLTLPGLH
                     QADSRRVALETYPGFSAREMVRGSYKSDALAKQTPARRQRRTRLVRLLTESGNPFEFV
                     LTAPTSLLSSLVSDPTGDRLDAVLCAMQAAWAWERREKNYGLPADVDVLEGWIAMVPR
                     PPAG"
     gene            complement(6498..6845)
                     /locus_tag="VN496_11300"
     CDS             complement(6498..6845)
                     /locus_tag="VN496_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17584.1"
                     /translation="MAQQKIEPRSVISNLEALLAKGKESALLRYSLGNEYLKLREQLV
                     AIVHLKRALQLDPEYSAAWRLLGTALVDAGILNEALDSYRRGIEVAQRRGDQQAAKEM
                     TIFARRLEKKLSP"
     gene            complement(6849..8735)
                     /locus_tag="VN496_11305"
     CDS             complement(6849..8735)
                     /locus_tag="VN496_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXF17585.1"
                     /translation="MSTNGGQIEKAGVWPLAIAAIGVVFGDIGTSPLYAMREAFNGSH
                     PVAANHDNVMGVLSLITWSLIIVVTFKYVIFMMRADNRGEGGIMALLALVLRSTSQDA
                     RGRWILMALGLFGAALFYGDGVITPAISVLSAVEGLEVATPALTPFVIPVTILVLIGL
                     FLFQQKGTEMVGALFGPITIVWFAVLALLGVINIVKYPDVLTAFFPSYAVHFFVANSF
                     GGFLVLGAVVLAITGTEALYADMGHFGKRPIRLAWSLYVLPALLLNYFGQGALILHDQ
                     AAVQHPFYHMAPDWALYPLVILATMATVIASQAVISGTYSLTRQAIQLGYCPRLEVTH
                     TSESEIGQIYMPWINWVLLAAVIGLVLGFESSSNLAGAYGIAVTGTMAIDTVLAFVLM
                     RKFWRWPLWVAVPLLLAFLTIDLSFFSANAIKLFHGGWFPIFVAIALFVLLITWKRGR
                     KLLMERLSPGAIPIEPFIHSVTAHPPTRVPGTAVFLTAAAEGVPHALLHNLNHNKVLH
                     ERIVLLTVTVSDIPHIGDEDRIEVVPLGTEFYRVIVHYGFKDDPDVPRALELAAAKGL
                     KFRMMETSFFVSRQTLIPTVGPGMALWQEKLFALMSRNASSATTFFKIPANRVVELGT
                     RIEL"
     gene            8982..9362
                     /locus_tag="VN496_11310"
     CDS             8982..9362
                     /locus_tag="VN496_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXF17586.1"
                     /translation="MTKQAIHTPDAPLAIGTYSQAIRVGAVVYLSGQIGLDPSTMQLV
                     DGFDAQAHRIFLNLRAVAAAAGLNLDDAVKMTVFMTDLANFGRLNEIMAHYLKQPYPS
                     RSAVGVAQLPRGALLEIEAVLVQS"
     gene            9558..11594
                     /gene="recG"
                     /locus_tag="VN496_11315"
     CDS             9558..11594
                     /gene="recG"
                     /locus_tag="VN496_11315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXF17587.1"
                     /translation="MQFAPPAICAKLAKIGIYQLSDLVTHLPLRYEDETRIVPIHDAV
                     SGESVQIEGTVVDTTIKFRPGRQLISEVDDGSGRLVMRFFNFYPSQRNALAPGTKVRV
                     MGEIRQGFFGAEMVHPRFRVVRGEMPLPKSLTPVYSTTAGLGQDVLRKLAVRALEQSD
                     LGDALPVPVRRKLQLLPLDEAIRLLHNPTPDVDADTLLDGTHAAWRRVKFDELLAQQL
                     SMRLHHDRRNRARAHPLLQHSKLSRILLAGLSFSLTKAQVRVLTEIRTDLTRPHPMQR
                     LLQGDVGSGKTVVAALASLQAIESGYQAAVMAPTEILAEQHYRKFTAWLAPLDIDVVW
                     LASSLKKKERESALSAIASGKAALAVGTHALFQDQVTFNKLGLAIVDEQHRFGVHQRL
                     ALRNKGSRAGQALQPHQLMMSATPIPRTLAMSYYADLDVSVIDELPPGRTPVMTRLLS
                     DTRREQVIERLHGHFELGRQAYWVCPLVEESEALQLQTAISTYEGLVGKFPGFRIGLV
                     HGRLTQQQKSDVMEAFQSGKINLLVATTVIEVGVDVPNASLMIIENAERMGLSQLHQL
                     RGRVGRGVLASDCILLYQNPLSQNARDRLRIIFENTDGFEIARHDLRLRGPGELLGAR
                     QSGVPMLRFADLDKDMDLLEVARELAPQLLRDYPANAERHMRRWLGGRQELLKV"
     gene            11661..12533
                     /gene="ubiA"
                     /locus_tag="VN496_11320"
     CDS             11661..12533
                     /gene="ubiA"
                     /locus_tag="VN496_11320"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HXF17588.1"
                     /translation="MTLAERLDAYEKLIRLDKPAGTLLLLWPTLWALWLSSRGHPDWS
                     LVLIFVVGTVLMRSAGCAINDYADRDFDGKVARTRDRPLATGQIMPIEALMVALVLIL
                     VSFALVVQLNQLTIKLSVVAVIIAAIYPFTKRFFAVPQAVLGLAFSFGIPMAFAAHTN
                     HVPWQAWVLLLANVFWTLAYDTEYAMVDREDDLKVGIRTSAITFGRFDIAFVMACHAL
                     FLLTMLWVGKLFRPGWHFYVGVGVAAVLVAFQYGMIRTRKPNDCFRAFGQNNWVGAVI
                     FAGIVASFYLQKHI"
     gene            12595..13368
                     /locus_tag="VN496_11325"
     CDS             12595..13368
                     /locus_tag="VN496_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17589.1"
                     /translation="MDLGLNRRAVLITGGSKGIGLACAHAFLREGATIAIASRDDANL
                     KAAGAQLGKVVLIKADLTKSEEAAHMVRQAEAALGPIDVLINSAGAAKRYPPEDLNAQ
                     AWHAAMDAKYFTYVHAMDAVLPSMRARKRGNVVNIIGTGGKVASSIHLPGGAANSALM
                     LASVGLAAVYGKFGVRINAINPGATLTNRIREALELEAATKGTTVQQALEQGQARVPL
                     GRYADPMEIASVALFLASDQAAYVTGAVIPMDGGVNPII"
     gene            13372..14835
                     /gene="ubiD"
                     /locus_tag="VN496_11330"
     CDS             13372..14835
                     /gene="ubiD"
                     /locus_tag="VN496_11330"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HXF17590.1"
                     /translation="MRYRDLRDFIRQLESHGELARITAPVSPNLEMTEICDRVLKAQG
                     PALLFEQPKGYSMPVLANLFGTPKRVAMGMGEDSVGALREVGKLLAYLKEPEPPSGLK
                     DAWSKLPVLRQVMSMAPKVVDDAPCHQIVLEGKDVDLARLPVQTCWPGDAGPLITWGL
                     TVTRGPRKARQNLGIYRQQVIGRDKVIMRWLAHRGGALDFRDHCESNPGQAFPVAVAL
                     GADPATILASVTPVPDALSEYQFAGLLRGSKTELVNCIGSDLQVPATSEIVLEGVIRP
                     GETALEGPFGDHTGYYNEQENFPVFTIERITQRREPIYHSTYTGKPPDEPSVLGVALN
                     EVFVPLLIKQFPEIVDFYLPPEGCSYRIAVVSMRKQYAGHAKRVMFGIWSYLRQFMYT
                     KFIIVVDDDIDVRDWKEVIWAVTTRVDPARDLLIAENTPIDYLDFASPVPGLGSKMGL
                     DATNKWPGETARNWGVPIEMNVAVKRHIDDIWFSLGL"
     gene            15049..15366
                     /locus_tag="VN496_11335"
     CDS             15049..15366
                     /locus_tag="VN496_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF17591.1"
                     /translation="MNKVVIIAAAVGLVLSSGAHAVDAEKAKALAQSKNCLACHAIDK
                     KLVGPAYTEVSKKYKGNKDAEAMLIKKVINGGGGVWGTIPMPPNPVKEDEAKVLVDWV
                     LSL"
     gene            15574..16776
                     /locus_tag="VN496_11340"
     CDS             15574..16776
                     /locus_tag="VN496_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF17592.1"
                     /translation="MGVVIIAAAAFGLTGCGKKEEPAAGTNKAASVQRVVKIGHAAPL
                     TGGIAHLGKDNENGVRLAVDEANAKALTIGGETVSFEMLSEDDEGKENKGPIIAQKFV
                     DAKVAGVVGHLNSGVTIPASAVYNQAGIPMISGSATNPKLTEQGFKVTFRTVGRDDQQ
                     GPAIASYLVSQHKPKSVAVIDDATAYGEGLANEVEKTLKAAGVNVLPREKGTQETRDW
                     KAILTKLKGKSPDAVFYGGMDSGAGPLLKQGRELGVKAVFAFGDGACTDKMKELAGQA
                     SEGLVCSQAGIPVQAASKAFLEAFKKKFNSDPLIYAPFTYDAANLLIAAMQVANSVDP
                     AKYLPSLAAIKYDGASGHIEFDAKGDRKDAEMTIFTMKNGTITPVAIIKGGQSVKFED
                     FVKGGATK"
     gene            16921..17907
                     /locus_tag="VN496_11345"
     CDS             16921..17907
                     /locus_tag="VN496_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17593.1"
                     /translation="MDIFLQQLINGLTLGSVYAIVALGYTMVYGIVQLINFAHGEVVM
                     VGAMVALWVISALAGTALPPLLIVSIGVLCAVPICMAVGFTMERIAYRPLRTAPRLAP
                     LITAIGISIILQQVAMIVFSRNPLGFPQIMDNPIFELAGAHISTVQIAIILGSVLMMA
                     GLTVLVYRTRLGIAMRATSENPQIAGLMGINADRVISATFVIGAGLAAVAGVMVGTYY
                     GIAHYTMGALLGLKAFSAAVLGGIGNLAGAMLGGILLGVVEALGAGYIGEIMDLCHLS
                     SISDTLAERCARDGHVILFGSNYQDVFAFLVLIAVLVFRPSGLLGERISDRA"
     gene            17911..19002
                     /locus_tag="VN496_11350"
     CDS             17911..19002
                     /locus_tag="VN496_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17594.1"
                     /translation="MTSWIARFKQLKSHRAAPWLGIALIAAALAVLPFVLASVGTAWV
                     RITNLAILFALLSLGLNIVVGFAGLLDLGYIAFYAVGAYCYALLASPHFGLHLPFWII
                     LPIGAALACLFGVLLGAPTLKLRGDYLAIVTLGFGEIVRVFLNNLSQPVNITNGPQGV
                     TLIDPFSIDGVNFNRTDTLVGLAFSGPIKYYYLLLLVLMAAIVINLRLQNSRLGRAWV
                     AIREDEVAAQAMGINTRNVKLLAFAMGASFGGIAGGIFAATQGFISPESFVFVESIMV
                     LSMVVLGGMGNVWGVVLGAILLSFVPEILRYTVEPAQKAVFGRLLIEPEVIRMLLFGL
                     ALVLMMLYRPAGLWPSKRREREFSETDHG"
     gene            19002..19775
                     /locus_tag="VN496_11355"
     CDS             19002..19775
                     /locus_tag="VN496_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17595.1"
                     /translation="MQTAPLLAIHRIEKRFGGLIALADVSLTINRNEIYGLIGPNGAG
                     KTTLFNVLTGLYVAEQGAFQFDGMDLMGLKPHRVAQYGIARTFQNIRLFGNMTAMENV
                     MVGRHVRTRAGVIGAILRTSRARKEEAAIRARSRELLRYVGIARHAGMLSRNLSYGDQ
                     RRLEIARALATEPKLLALDEPAAGMNATETVELRQLLETIRNDGVTILLIEHDVKLVM
                     GLCDRVAVLDYGRKIAEGEPLQVQRNPDVIRAYLGGALP"
     gene            19772..20482
                     /locus_tag="VN496_11360"
     CDS             19772..20482
                     /locus_tag="VN496_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17596.1"
                     /translation="MNTLLDVKALKVAYGGINAVKGVDLTVGAGEMVALIGANGAGKT
                     TTLKAICGLLPHAGVVKLRGENVNSCSAYQLVARGLVLVPEGRGVFGRLTVHENLQIG
                     AYMRRDYATIRSDLERIYGLFPHLAQRRTQTGGTLSGGEQQMLAIGRALMSRPKLLLL
                     DEPSMGLAPLMVEKIFDTVRMVSREGVTVLLVEQNAKLALEASNRGYVMESGLITLSD
                     GAPALLNNPRVRTAYLGE"
BASE COUNT         4299 a         5713 c         6088 g         4434 t
ORIGIN      
        1 cttgcgcgct ttgaagttcc cgaggagttc aagccgttgc tgccgcacct cctttaccag
       61 cctgataaag gatgcattga atacaaggcg ctcgaacaag ccgctgcgct ggcgcacctg
      121 accattccga ggctgctgga aagaagcggt gcgtttgctt cccctcgcga ctaccacttc
      181 ggacgttttc tgttcgagta ttttccgggt ggcacgtcat tcgatccagg tctggtcgcg
      241 atgccgcccg gcgcgctacc gctatcgccg gctgagtgtt tcagcatcga tgatgccacc
      301 acgacggaaa tcgacgacgc tttttcggtc acgaagctcg cgaacggcaa ctggctggtg
      361 ggcgtgcata ttgcggcgcc tgcgttgggc attgcgcccg gatcgccgct cgaccgcgaa
      421 gccgccaaac ggctgtccac cgtatatttt cccggcgaca agatcaccat gctgccagag
      481 aacgccatcg cgcaatacac gctgtcggaa ggtggcgatt gtccggccct gtccatgtac
      541 atcgaagtga cgccgcagtt cgaggttgtc gcacaccgca gcgttgtaga acgcgtgcat
      601 attgcggcga acttgcgttt cgacgaactc gagactgaat tcaacgagca gcaaatcgcg
      661 agcgggcaga tatcccatcg ttttggcgag cagctgatgg tgctgtggca tctggccgcc
      721 aggctggagc gccagcgcgg caaagatgaa gtggccaatg cccgatccga ttacaacttt
      781 cacgtggaaa acgatcgcat cagcatcgtg gctcgccggc gaggctctcc ggtcgataaa
      841 gtggtttcgg aattgatgat cctggtcaac acgcaatggg gcggcgagct ggcacagaac
      901 gggattccgg cgctgtatcg tgcgcaatcc ggcggcaagg tcaagatgtc gagcgtgcct
      961 gcaccgcacc agggccttgg tgtacctcaa tacatctggg ccagctcgcc tctgcgtcgt
     1021 tatgcggatc tggtcaacca gcgccagttg ttgtcgctga tcagcaacga agctgtgccc
     1081 tatcctgccg gggatgagcg cttgctcgtc atcctgcgcg atttcgaact ggcctatgac
     1141 gcctacgcgg aattccagcg cggcatggaa cgctactggt gcctgcgctg gctgattcag
     1201 gaagatatca gcttgactgc ggcggaggtg ttgcgtgagg aattggtgcg aatcgagcgt
     1261 attccgctag tggtgcgtgt tccatcaatg ccggccacat cgccaggcac ccgcgtggag
     1321 ctcgtgcttt ccaacatcga cctgctcgat ttgacagtgc attgcgagtt caagcagacc
     1381 ctcgaagcgc tatcgcgctg acagttgcgg cttagttatt tagaattgac gctggctcaa
     1441 tcatccaagg ctcaattgcc ggatacgtcg atcagcgcac ttcccgcacc gggcagccta
     1501 gccgctggcc ttgccatttc tccgccaccc gcttcgggtg acatgctgcc gataactctg
     1561 gcgatctcgg tcgtcctgca cgcagtcgtt ttgctgatca cgtttgcacc gcccgatttc
     1621 gacagctcta aatttgcgcc gcatcttgat gttgtgctgg tcaattcaaa atcagtttcc
     1681 aggccgttga aggcggacgc gctggcgcaa gccaatctgg atggaggcgg aaataccgat
     1741 gaagatcggc gcgccaagac gcatttgcca aagctgaatg atgtcgaacc ggcacgcgac
     1801 gtccagctca gttcaaggca ggtacgccgc ctggaacaag aggcgcaacg cctgctgctg
     1861 ctgtcgaaag gcacgcatgc aacttctgat tccacccagc cagcgccaga gttgccgcgt
     1921 gaggcacttc tggaagaccc ggcagccgag caacagcggc tgcgcatcgc acaattggaa
     1981 gcacagatcg ccaaggactg ggacgaatac cagaaaatgc cccggcgcaa gttcatcggc
     2041 gcgcgcacag aaggcgtggt ctacgcggaa tacgtcgacc ggtggcgcca gcgcgtggag
     2101 caggtcggca cgcaacattt cccggatgaa gccagacagc gcaaactgta cggctcgctg
     2161 gtcatgacgg ttcacatacg ggccgatggc agcgtggaaa aggtagaaat cgatcgctcg
     2221 tctggctacc gcctgctcga ttcggctgcg ctgcaagccg tggatcttgc cgggcccttt
     2281 ccgccctttc ccgccgcggt gcgcaaggaa tgggacatcc tcagcatatc gcgcactttt
     2341 agctatacac gcagcgacct tgaagtgctt gtgaatccat gacggatcgt tatgccgtcg
     2401 taggcaatcc ggtggcgcat agtaaatcgc ctgaaatcca cgcggcattt gcgcgccaga
     2461 ccgggcagga catcgagtac tcgaagttgc ttgctccacc aggcggattc cgctcgacca
     2521 tcgaagcatt tcgtgcggac ggaggccgag gcgtcaatgt cactgtcccg ttcaagctgg
     2581 aagcattcgc cctggcagac gatctatcgc aatttgcaag aaatgcacag gcggccaact
     2641 tgcttcagtt cgacggcgga cgaattcgcg cgcacaacac cgatggcatt ggcttgctgc
     2701 gcgatatcgt acataacctc ggttttggga ttgcggacaa acgcgtgctg ctgatgggag
     2761 cgggcggcgc tgcgcgcggc gtgcttacgc cgttgctgga gtacggacca cgcattctta
     2821 cgatcgcaaa ccggacgata gacaaggcgt tacgccttgc agagacattc cgccaccttc
     2881 cggccgcgtc atccagtgtg ctgagcggga tcggctatgg cgaactggtg ggtcatcatt
     2941 ttgacctggt aatcaacgcc acttcatcca gtttgaacgg cgaactgccg ccgctgcccg
     3001 ccgacctgtt tgccccggac gcgctcgcgt acgacatgat gtatggcaag accctgacgc
     3061 cttttctggc gttttccgcc gcacgcggcg caaaatattc tgacggactc ggcatgctcg
     3121 tagagcaggc cgcagaatct ttcctgcact ggcgcggcgt gcgtccccaa actgcgcctg
     3181 tcatcgaact gttgcgcaat tcatgagcgc gatgagacgc gcctggcgtt ggcccgcacg
     3241 gattatgctg gtgctgattg ctgcggtcgt ggtctatcag ttctggatca tgggcagtgt
     3301 cgtgtggtgg gtctggttca atccgtccac cagtgcgttc atggcagatc gccttgaggc
     3361 tctgcagctg catgatccgg atgcggtatt gcatcatcaa tgggtaccct atgagcacat
     3421 atcaatcaat ctgaagcgcg ccctgattgc ggcggaagat gccaagttcc tggatcatga
     3481 cggcttcgac tgggagggaa tccagaaagc ctacgaaaag aacctgaaga aaggccgtgt
     3541 agtcgcgggc ggctcgacca tcagccagca actcgccaag aacctcttcc tctccggcag
     3601 gcggacgccg tggcgcaagg cagaggaagc gctaattacg atgatgctgg aaaacatgat
     3661 gtcaaagcgc cgcatcttcg aaatctattt gaatatcatc gaatggggca atggcgtgtt
     3721 cggtgcgcag gctgcagcgc attattatta cggcaccagc gcgagcaatc tgacggccga
     3781 acaagcggca cggttggcgg cgatggtgcc gaaaccacgt tactacgaca aagtgcgtga
     3841 cacaccgctg ctggaacggc aaacggctat catccttgag cgcatgtggc aagtcgccgt
     3901 cccgtagccg ggcgcactat aatttcgcgc catgctgatc catccgcagt tcgatcccgt
     3961 ccttgtacac ctcggtcccc ttgccattcg ctggtacggg ctcatgtatt tgctggcatt
     4021 cctgctggta ctggtcctcg gacgctatcg cattcgtacg cagccctggc gcgacgtgag
     4081 cgtgcgcgat ctggacgata tgttgttcat gggcgtggtg ggcgtcgtgg ttggtggacg
     4141 gctgggatat atcctgttct acaagctcgc ggactacatg aaggagccgt tgcatatttt
     4201 ttatgtctgg gaaggcggca tgtcatttca cggcggcttt ctcggtgtaa tccttgcgat
     4261 gctttggttt gcgcgttctc gcggaaaacg ctggcttgcg ctgacggact ttattgcgcc
     4321 gctggtgccg cttgggctgg gtgccgggcg tctgggtaat ttcatcaatg cggaattgtg
     4381 gggacgcccg accgatgtgc cgtgggcgat gatctttcca aacatcgaca atgtgccacg
     4441 tcacccttcg cagctttacg agttcggcct ggaggggctg gtattgttcg gactgctctg
     4501 gtggttttcg agcaaaccgc ggcccacagg cgcggcgtcg ggtctgttct tgcttggtta
     4561 cggattactg cgcttcgtgg ttgagtacag tcgcgaaccc gatggttttc tcgggctgct
     4621 cgcgcttgga ttttcgatgg gacagtggct tagcctcccg atggcaataa cggggatggc
     4681 gttgttggcc tgggcgtatc gcaaaaaaac tagttgagct cgcgtcgctt ctcgggccgt
     4741 ttgactgcaa aaaaactgcg ccggtttagc aagcggcgga aattgtccag tgacagcagc
     4801 tgtgaataga ttttttccat cgcgccggcg gacatcagcc gccgcagctg atccccaggc
     4861 agcccctcca gcgcagcgcg agcaatccgg tgcatgccct ccagttgcat ggtgaaaccg
     4921 tccacctccg ccttcatggt gaagggttcg agcagtttca tttccacaag cagctggcaa
     4981 agcgactctg aacgcacgag gtcctgctca aaatcgaata tcagcttttc cagcaggatc
     5041 cactgcgcca gtgcttcgcc cctggtgtcg tacagcggct cgcccgactc ctgcaacgcg
     5101 cttttctcaa tgcagacgat gcgttccttc tgcggttctc ccgcgacgga gacccggctc
     5161 atacagaaag gatagcgccg cacgtaggcc ggcagatata cgcgtcggtc ccaggtgccg
     5221 tcagtgagga tgaacagatt ctggttgact tgcattccca gaaccagcac ggcactgaag
     5281 ttcttgccgc cgtcgttggt cagaaacaca attggataat cgcggcaagc cggttcaaat
     5341 tcggcgaggc tcaaaggcat gacgtgcagg cggtgaaaca ccgccggcgt ttcctcggcg
     5401 cgcggtagca aaaccttatg acttttaagc aacggcgtga tctgctcata gcccagcggg
     5461 ggactgattt tcattggtgg ttgcgtccga tttcgttgct tctgttttca gtataataat
     5521 gccgcattac cgtaacacgg atgcgcttat tcggggacgt agctcagctg ggagagcgtc
     5581 gcgttcgcaa tgcgaaggtc gggagttcga tcctcctcgt ctccaccacc gaagcatcag
     5641 accagttcaa ccggcgggtg gtcgcggcac cattgcaatc catccttcaa gtacatccac
     5701 gtcagccggc agcccgtaat ttttttcgcg acgttcccac gcccacgcag cctgcatggc
     5761 acacagcacc gcatccagcc gatcaccggt cgggtcgctg accagtgaac tgagaagact
     5821 agtgggcgcg gtcaatacaa attcaaacgg attgcccgat tcagtaagca ggcgcaccag
     5881 gcgggttcgt ctttgccggc gggctggcgt ctgcttggct aacgcatcgc ttttgtatga
     5941 tccccggacc atttcacggg ccgaaaaacc gggataagtt tccagtgcga cgcgtcgcga
     6001 atccgcctga tgcaagcccg ggagtgtcag gccggcatcc atcagccgcg gtacgccttc
     6061 caaaaacatc aaagcgaccg gcggattcac cagcttgagc ggactgtgag accccgcagg
     6121 atgatctgcg gcgcgatgcg cataacgttt tccgaccggc cggctttctc gatatttatc
     6181 cagcaaggca cggaatgctt cgcgtccctg ggcacggcag tggtccacca gttgcggata
     6241 gctgtgcgga agaccaagat cgtcgactgc ttcgcgcggt aagccaaacg ggaaatcgaa
     6301 gccagcaatc caggggcctg gacgtcgcag ccagcattcg aaaccgggcc agtcgataat
     6361 ttcgttcacg gcgtcgagcc gaaacgtgcg gcttgtccgg ctgccgctgg caacagtaat
     6421 cggcttcttc cggcgtggac ggctggtgaa gtccacaccg tggattggcc agcgcgccgg
     6481 ttcacgcata atgcgattca gggcgaaagt tttttttcga gtcggcgggc gaagatcgtc
     6541 atttctttcg ccgcctgctg gtctccgcgc cgctgagcca cttcgatgcc gcgtcggtag
     6601 ctatccaatg cctcgttcag aatccccgca tccacaagcg ccgtgcccag cagcctccat
     6661 gccgcggagt attctggatc gagctggagt gcgcgcttca gatgcacgat ggcgaccaat
     6721 tgttcgcgca gctttagata ctcgttgcca aggctatatc tgagcaatgc cgactccttg
     6781 ccttttgcaa gcagcgcttc cagattggaa atcactgagc gcggctcgat cttctgttgt
     6841 gccatggcct acaactcgat tcgtgtgccg agttcaacta ccctattggc ggggatcttg
     6901 aagaacgtag tcgcacttga agcattgcgt gacatgagcg caaaaagttt ttcctgccat
     6961 agcgccatgc cggggccaac tgttggtatc agcgtctgcc ggctaacgaa aaacgacgtc
     7021 tccatcatgc gaaatttcaa tcccttcgcg gcagccaatt cgagtgcgcg agggacatcg
     7081 ggatcgtcct tgaaaccgta atgcacgatg acgcggtaga actctgtacc cagtggcacg
     7141 acttctatgc ggtcttcgtc ccctatgtgc gggatatcgc tgacagttac cgtgagcagc
     7201 acgatgcgct cgtgaagcac tttattatga ttgagattgt gcagtagcgc gtgtggcacg
     7261 ccttccgcgg ctgctgtaag gaagacggcc gtgcccggta cccgcgtcgg tggatgcgcg
     7321 gtgacgctat gaatgaaggg ttcgattgga atggcgcccg gagaaagccg ttccatgagc
     7381 agtttgcgac cgcgcttcca ggtaatcaaa agtacgaata aagcgatggc gacaaaaatc
     7441 ggaaaccacc cgccatggaa tagcttgatc gcgtttgcgc tgaagaatga caaatcaatg
     7501 gtcagaaatg ccagcagtaa cggcacggcg acccacaagg gccagcgcca gaatttacgc
     7561 atcagcacga acgcgagcac tgtgtcgatc gccatggtgc ccgtcactgc gatcccgtac
     7621 gcgcctgcga gattactgga agattcgaaa cccagcacca ggccgattac cgctgcaagc
     7681 agtacccagt tgatccaggg catgtatatt tgcccgattt cgctctcgga ggtatgcgta
     7741 acttccagcc gcggacagta gccgagctgt atggcctggc gggtcagcga gtaagttccg
     7801 gaaatcactg cttgtgatgc gattacagtc gccatggtgg cgaggataac caggggatac
     7861 agcgcccaat ccggagccat gtggtaaaag ggatgctgca ccgctgcctg atcgtggaga
     7921 atcagcgcgc cttgaccaaa gtaattcaga agcaaggcgg gcaacacgta gagtgaccac
     7981 gccagcctga tcggcctttt accgaagtgt cccatgtcgg catacaaagc ttcggtgcca
     8041 gtgattgcca ataccaccgc gccgagcaca aggaagccgc cgaatgagtt ggcgacgaag
     8101 aaatgtacgg cgtacgacgg aaaaaaggcg gtcagcacat ccggatattt cacaatgttt
     8161 atgacgccta gcagagcgag cactgcgaac cagacaatcg taatggggcc aaacaacgcg
     8221 ccaaccattt cggtgccttt ctgctggaac agaaacaggc ctatcagtac caaaatggtg
     8281 accggaataa cgaaaggcgt gagtgcaggc gtagcaactt ccaaaccttc aacggccgaa
     8341 agcaccgaga tcgccggcgt gatgactccg tcgccgtaaa acaaagctgc gccaaacagc
     8401 ccaagcgcca tcaggatcca gcgtccccgc gcatcctggc tggtggaacg caagaccagc
     8461 gcaagcaacg ccatgatgcc gccctcgccg cgattgtctg cgcgcatcat gaatatcacg
     8521 tacttgaaag tcacaacgat gatcagcgac catgtaataa gggacaagac acccattacg
     8581 ttgtcatggt ttgctgcaac cgggtggctg ccgttgaatg cttcccgcat ggcgtacaag
     8641 gggctggtgc cgatatcgcc aaacaccacg ccaatggctg cgatcgccag cggccacacc
     8701 ccggcttttt cgatctggcc gccattggtg ctcattgtta ttcttaattc ctcattggcc
     8761 acagtttgat ctcgcttggc gaaggcatgg gattgccggc ggtgcagtca cttagctggt
     8821 cgttgtcggg caggataaag gtgcaacgat ttcacgccgt gacggtgaaa ccgccgcttg
     8881 ctgttcggtt atcatttaag gacgctacgc cagcccgatg gatgattggt cggtggccga
     8941 ccctcttatt gtggcgcaac attctatacc tggagcaggt tgtgaccaaa caggcaatcc
     9001 acaccccgga tgcgccgctg gcgatcggaa cctattccca ggcgatacgc gttggcgctg
     9061 ttgtctatct ttcggggcag atcggcctgg atccttctac gatgcaactg gtggacggct
     9121 tcgacgcgca agcgcatcgc attttcctga atctgcgagc cgttgcggcc gctgcaggcc
     9181 tgaacctgga tgatgcagta aaaatgaccg tgttcatgac cgatcttgca aattttggcc
     9241 gtttgaacga aatcatggcg cactatttga agcagccgta tccatcgcgg tcggcagtcg
     9301 gagtcgcgca attgccacgc ggagcgctgc ttgaaataga ggccgttctg gtgcaatcgt
     9361 gaggcgcaag cggtgacgca tgtgcagtgc cgtcgagacc gtttcacgcc tctgttacaa
     9421 acagcgattg ttgcgtgaaa caggccgtcc ttcagaaggg caagcgaccc aatgggcgca
     9481 acagtaaacg acgcgcgagc atcgcttccg ccaaagcagg cacttctcgc gcggcaagcg
     9541 ctgcagcaca tccgtcgctg caattcgccc caccggcgat ctgcgcaaag ctggcgaaga
     9601 tcggtattta tcagctgtct gatctggtca cgcatctgcc attgcggtac gaggacgaga
     9661 cccgcatcgt tcccatccac gacgctgttt caggggagtc cgtgcaaatt gaaggtacgg
     9721 tggttgatac caccatcaaa ttccgaccag gacgccaact gatttcggag gtggacgacg
     9781 gcagcggccg gctggtgatg cggtttttca atttctatcc aagtcagcgg aatgcgctgg
     9841 cgcctggaac caaagtgcgc gtcatgggcg aaatccgcca ggggttcttt ggcgcggaaa
     9901 tggtccatcc gcgttttcgc gtcgtgcgcg gcgaaatgcc gctgcccaaa tcgctgacac
     9961 cggtctattc aacaactgcc ggtctcggac aggatgttct tcgcaagctc gctgtacgcg
    10021 ccctggagca atccgatctt ggagacgcgc tgcctgtgcc ggtgcgcagg aaattgcagc
    10081 ttcttccgct cgatgaggcc atccggctgc tgcacaaccc tacgcccgac gttgatgcgg
    10141 acacgctcct ggacggtacc catgcggctt ggcggcgcgt gaaattcgac gagctgttgg
    10201 cacagcaact atcgatgcga cttcaccacg accgccgaaa tcgcgcgcgc gcgcatcccc
    10261 tgctgcagca ttccaaactc tccaggattc tgctcgccgg tctgtcgttc tcactaacca
    10321 aagcacaagt gcgcgtgctc actgaaatcc gcacggatct gacccggccc cacccgatgc
    10381 agcggctgct tcagggcgat gtcggtagcg gcaaaacagt cgtcgcggca ctggcctcgc
    10441 tgcaggccat tgaatccggg tatcaggccg cggtcatggc gccgaccgaa atacttgctg
    10501 aacagcacta ccgaaagttc acggcctggc ttgccccgct cgatatcgat gtggtctggc
    10561 tcgccagcag cctcaaaaaa aaagagcggg aatccgcgct gtcggcaatc gcctccggca
    10621 aagccgcact tgcagtaggc acgcacgcgc tgttccagga tcaggtcaca ttcaataaac
    10681 tcggacttgc aatcgtggac gagcagcacc ggttcggcgt gcatcagcga ttggcactgc
    10741 gcaataaggg cagtcgcgcc ggtcaagcac ttcagccgca ccagctaatg atgagcgcca
    10801 ctcccatccc gcgcacgctg gcaatgagct attacgccga tctcgatgtg tcggtcatcg
    10861 atgaactgcc tcccggacgc acgccggtca tgacgcgcct gttgtccgat acgcgccggg
    10921 agcaggtgat cgagcggctg catggtcatt ttgaacttgg gaggcaggct tactgggttt
    10981 gtcccctggt tgaagaatcg gaagcgttgc agctgcaaac cgcaattagt acctatgagg
    11041 ggctggtggg caaatttccg gggttcagaa taggcttggt ccatggacgt cttacgcagc
    11101 agcagaaatc cgacgtcatg gaagcttttc agtcaggcaa gatcaatctg ctggtggcga
    11161 ccacggtcat cgaagtgggg gttgacgtgc ctaacgcatc cctgatgatc atcgaaaatg
    11221 ccgaacgcat gggcttgtct cagctacacc aactgcgggg tcgcgtgggg cggggagtcc
    11281 ttgcgagcga ttgcattctg ctctaccaga acccgctgtc gcaaaatgcg cgtgaccgac
    11341 tgcgcattat ctttgaaaat acagatggct ttgagattgc acggcacgat ctccgcctgc
    11401 gcggccccgg cgaattgctg ggcgcgcgcc aaagcggcgt acccatgttg cggttcgcgg
    11461 acttggacaa agacatggat ctgctcgagg tcgcacgcga actggcgccg cagttgctgc
    11521 gcgactaccc ggccaacgcc gagcggcata tgcggcgctg gctcggcggg cgacaggaac
    11581 tgctcaaggt ataggaagaa gctcggtctc tatgcgatca tgcagtcgtt aatgacaaat
    11641 gacagtgacc accgattgcg atgactttag cagagcgcct ggatgcctat gagaaactga
    11701 tccgtctgga caagccggct ggcacattgc tcctgctctg gcctacgttg tgggcgttgt
    11761 ggctgtcctc gcggggacat cctgactgga gccttgtgtt gatattcgtc gtcggtaccg
    11821 tgctgatgcg ctcggcgggc tgcgccatca atgattacgc ggaccgcgat ttcgatggca
    11881 aggtcgcgag aacccgggac cgtcccttgg ccactggtca gatcatgcca atcgaagcat
    11941 tgatggtggc gctggtactg attctggttt ccttcgcgct ggtcgtgcag ctgaatcaac
    12001 tgaccatcaa attgtccgtc gtcgccgtca taatcgcagc gatctatccg ttcacaaaac
    12061 ggttctttgc ggtgccgcaa gccgtgttgg gactcgcgtt cagttttggc atcccgatgg
    12121 cctttgctgc acacaccaat catgtcccgt ggcaagcttg ggtgctcttg cttgccaacg
    12181 tgttctggac gcttgcgtac gataccgaat atgccatggt ggaccgggaa gacgatctga
    12241 aagtcgggat tcgcacttct gcgattacct tcggccgctt tgatatcgcc ttcgtgatgg
    12301 cgtgccatgc gctgtttttg ctgaccatgc tttgggtcgg caagctgttc cggccgggct
    12361 ggcattttta cgtcggtgtc ggtgtggcag cggtgctggt cgcatttcag tacggcatga
    12421 ttcggactcg caagccgaac gattgctttc gcgcgttcgg gcagaataat tgggtgggcg
    12481 ccgttatctt tgctggcata gtcgccagct tctatctgca aaagcatatt tgaaccgctc
    12541 ggctggtgcc gcatcgcggc ttgagcaatg gcgtgaataa tctaacggct gaccatggat
    12601 ctcggattaa atcgtcgtgc ggtgttgatt accggaggaa gcaaaggaat agggctggct
    12661 tgcgctcatg cattcctgcg ggaaggcgca acaatcgcaa tcgcctcgcg cgatgacgca
    12721 aatcttaaag cggccggtgc acaactcgga aaagtcgtcc tgatcaaggc cgatctaacg
    12781 aagagcgagg aagcagcgca tatggtccgg caagcggaag cagcgctcgg tccgattgat
    12841 gtactgatca acagtgccgg agcggcgaag cgctatccgc ccgaagatct caatgcgcaa
    12901 gcctggcatg ccgcaatgga cgcgaagtac ttcacgtacg tccatgcgat ggatgccgtg
    12961 ctgccctcga tgcgcgcgcg caagcgcggc aacgtagtca acatcattgg caccgggggc
    13021 aaagtggcgt cgtcaataca tctgccaggc ggagcggcaa attcagcgtt gatgcttgca
    13081 tcggtcggtc tggcggctgt gtacgggaaa tttggcgtgc gtatcaatgc gatcaacccc
    13141 ggtgcaacgc tcaccaaccg catccgcgaa gcgctggaac tggaggcagc aaccaaaggc
    13201 acgacggtac aacaggcact cgagcaaggc caggcgcgcg tgccgttggg gcgctacgcc
    13261 gaccccatgg aaattgccag cgttgcgctg ttcctggcct cagaccaggc tgcatacgta
    13321 acgggcgcgg tcattccgat ggacggtggc gttaatccga ttatttgagc catgcgttac
    13381 cgggatctcc gcgatttcat caggcaactc gaaagccacg gagaactcgc acggattacg
    13441 gcaccggtct cgccaaatct cgagatgacc gaaatttgtg acagggtact gaaagcccag
    13501 ggacccgctc tattgttcga gcaacccaag gggtattcca tgcccgtttt ggcaaatttg
    13561 tttggcacgc caaagcgggt ggcgatgggc atgggagagg attccgtcgg cgcattgcgg
    13621 gaggtcggca agctgctcgc ttatttgaag gagccagagc cgccgtccgg tttgaaggac
    13681 gcctggagca aattaccggt gctgcggcaa gtgatgtcta tggcgccaaa ggtagttgac
    13741 gatgcgccgt gccatcagat cgtgctggaa ggcaaagatg tggatctggc ccggctgcca
    13801 gtgcaaacgt gttggccggg ggacgctggc ccgctgataa cctggggtct gacggtcacc
    13861 cgtggacccc ggaaagcgcg tcaaaatctg ggcatttatc gtcagcaggt aatcggtcgc
    13921 gacaaagtga tcatgcgctg gctcgcacat cgcggcggcg cgctcgattt tcgggatcat
    13981 tgcgaatcca atccgggcca ggcgtttccg gttgcagtag cgctaggcgc agacccggct
    14041 accatacttg cgtccgttac gcccgtgcct gatgctttgt cggagtacca gttcgccgga
    14101 ctattgcgcg gctcgaaaac cgaactggtg aattgcattg gctccgatct ccaggtgccg
    14161 gcgacaagcg agatcgtgct ggaaggcgtt attcggccag gcgaaaccgc gctcgagggg
    14221 ccgtttggcg accacactgg ttactacaac gaacaggaga attttccggt atttaccatc
    14281 gaacgcatta cccaacgccg cgaaccgatt tatcacagca cttacaccgg gaaaccgccg
    14341 gacgagccgt ctgtgctcgg tgtcgcgctg aacgaagtat tcgtgccgct gctgatcaag
    14401 caatttcccg agatagtgga cttctatctg ccgccggaag gctgctcgta ccgcattgcg
    14461 gtggtcagca tgcgcaagca atatgccgga catgccaagc gcgttatgtt tggcatctgg
    14521 tcgtacctgc gccagttcat gtataccaag ttcatcatcg tggtggacga tgacattgac
    14581 gtgcgcgact ggaaagaagt gatctgggct gtgaccacac gtgtggaccc ggcgcgcgat
    14641 ctgctcatcg cggaaaatac gccgatcgac tatctggatt ttgcaagccc ggtcccgggg
    14701 cttggctcaa aaatgggact cgatgcgacc aacaagtggc ccggcgaaac tgcccgcaac
    14761 tggggcgtac ccattgagat gaatgtggcg gttaaacgcc acatcgatga catctggttt
    14821 agcctcggtc tatgagagcg gccgttcacg cccggaaaag ccgccccgcc aatgcgtaca
    14881 ggatattgtc aacgcttatc ccagccgtag cgttatttgc gcgtggtcac ttgagcggtg
    14941 caacaggctg cgaacctcgg acttggatca atgtctgagt tctcaaagca gcccaaagcc
    15001 gatagactgt ctcgcgttct aaacccactt tgtagaaagg cgctcgtaat gaataaggtc
    15061 gtgataatcg cagcggcagt aggtttggtg ctctcctctg gcgcgcatgc ggttgatgcg
    15121 gaaaaagcaa aggcgctggc gcagtccaag aactgtctgg cctgtcatgc gatcgacaag
    15181 aagcttgtcg gtcctgcata taccgaggtg tcaaagaagt acaaaggcaa caaggacgcc
    15241 gaggccatgc tgatcaagaa agtgatcaac ggcggcggtg gcgtgtgggg cacgatcccg
    15301 atgccaccaa acccggtaaa ggaagacgag gcgaaggtgc tcgtcgattg ggtcttgtcg
    15361 ttgtaacagc aactggatca tcacaaaagg ccggcgtcag ccggcctttt gctttccgct
    15421 gctcgctaat acaagatgat gaattcacgg ctcaaattga caatgctcaa aatgctggcg
    15481 gataatcccg cgggtcatcg ggatatcggc cacaacaaac caaggggact tacatcaatg
    15541 aatgctaccg gcgtgaagaa aatatgcaga tggatgggcg tagtcattat cgccgcagca
    15601 gcattcggtc ttacgggttg tgggaagaag gaggaacctg ctgcgggtac caacaaggca
    15661 gcgtccgtgc agcgagtagt caagatcgga cacgccgcac ccttgactgg cggcattgct
    15721 cacttgggca aggataacga aaacggcgtg cgcctcgcgg tcgatgaggc caacgccaag
    15781 gcgttaacga taggcggtga aacggtctct ttcgagatgc tgagtgaaga cgacgaagga
    15841 aaggaaaata agggacccat catcgcgcaa aagtttgtgg acgcaaaagt agccggcgta
    15901 gtgggccacc tgaactctgg cgtcaccatt ccagcgtcgg cggtttacaa ccaggccggc
    15961 attcccatga tcagcggctc ggcgaccaat cctaaactga ccgagcaggg attcaaggtt
    16021 acgttccgta ccgtgggccg agatgatcag caagggccgg cgattgccag ctatctcgtc
    16081 tcccaacaca aaccgaaatc agtcgcggtc atcgacgatg cgactgctta tggcgagggg
    16141 cttgcgaatg aggtcgagaa gaccttgaag gcagccggtg tcaatgttct gccgcgcgaa
    16201 aaaggcaccc aggaaacgcg ggactggaaa gctattttga ccaagctgaa aggtaaaagc
    16261 ccggatgcgg ttttctacgg cggcatggat tcgggcgctg gtccgttgct gaagcaaggg
    16321 cgtgagctcg gcgtcaaagc cgtattcgct ttcggcgatg gagcatgtac ggacaagatg
    16381 aaggaactcg ccggccaggc ctctgaaggt ttggtctgct cgcaggcggg cattccggtg
    16441 caggcggcca gcaaggcctt tcttgaagca ttcaagaaaa aatttaattc tgatccgttg
    16501 atttacgcgc ccttcactta cgatgcagcc aacctgctca ttgcggccat gcaggtggcc
    16561 aattcagtcg atcccgccaa atatcttccc tcgcttgccg caatcaagta tgacggtgca
    16621 agtggccata tcgaattcga tgcaaagggc gaccgcaagg atgcggaaat gactattttc
    16681 acgatgaaga acggcaccat tacgccagtc gcaattatca agggtggcca gtccgtcaag
    16741 ttcgaggatt tcgtcaaggg cggagccacg aagtaaacgg tgaacgaacg aatggagaaa
    16801 acggcacctg cgggtgctgt ttttattcgt cgatccacac tgcgcgccgc tgtacctcaa
    16861 gagtcagtgc ggaacattgc tccggacaac gttcccgcgt gaatcccacg ggctaagatc
    16921 gtggacatct tcctgcagca attgatcaat ggtcttactc tgggcagcgt gtacgcgatc
    16981 gttgcgctgg gttacacaat ggtctacggc attgtgcaat tgatcaattt tgcacatggc
    17041 gaagtggtga tggtgggcgc catggttgca ctgtgggtca tttccgcact ggctggcact
    17101 gcattgccac cgctgctgat tgtctccatc ggcgtactgt gcgccgtacc gatttgcatg
    17161 gcggtgggtt ttacaatgga gcgcatcgct tatcgccctt tgcgaactgc tccgcggctg
    17221 gcgccgttga ttacggcgat tggcatctcg attattctgc agcaggttgc catgatcgtc
    17281 ttcagccgca atccgctggg atttccccaa atcatggata acccgatatt cgaactagcc
    17341 ggcgcacata tcagcaccgt gcagattgcc attatcttgg gttcagtact catgatggct
    17401 ggcttgacgg tgttggtcta tcgcacccga ctcggaattg ccatgcgtgc aacctcggaa
    17461 aatccgcaaa ttgctggctt gatgggaatc aatgccgacc gcgtcatttc ggcgactttt
    17521 gtcattggtg cgggactggc agcagttgcc ggcgtcatgg tcggaaccta ttacggtata
    17581 gcgcactaca ccatgggcgc attgcttggc ctgaaagcat tttcggccgc cgtacttggc
    17641 ggcattggca atcttgcagg cgcgatgctc ggcggcatcc tgctcggcgt ggtagaggcg
    17701 cttggcgccg gttacatcgg cgaaattatg gacctgtgcc acctcagcag catctcggat
    17761 acattggccg agcgctgcgc tcgggacgga cacgtaatcc tttttggcag caactatcag
    17821 gatgtttttg ccttcctggt gctcattgcc gtattggtat tccgcccttc gggtttgctg
    17881 ggcgagcgca tttcggaccg ggcctgacgc gtgacttcct ggatcgcccg cttcaaacaa
    17941 ctaaagtccc accgggccgc accatggctc ggcatcgccc tcattgcggc ggcattggcc
    18001 gttcttccat tcgtgctcgc ttccgtgggg acagcgtggg tgcgcattac caatcttgcc
    18061 attctgttcg cgctgctttc ccttggactc aacattgtcg ttggtttcgc cggactgctg
    18121 gatctcggct atatcgcgtt ttatgcggtc ggcgcttact gctatgcgtt gctcgcgtca
    18181 cctcacttcg ggttgcacct gccgttctgg atcatccttc cgatcggagc ggcgctggcc
    18241 tgcttgttcg gggtgttgct cggcgcgccc acccttaaat tgcgcggcga ttatctcgca
    18301 atcgtcacgc tcggatttgg cgagattgtg cgtgtgtttc tgaacaacct gtcccagccc
    18361 gtgaacatca caaatggccc acaaggcgtc acgctcatcg acccctttag catcgacggc
    18421 gtgaatttta accgcactga tacgctcgtc ggcctcgcat tcagcggacc gatcaagtac
    18481 tactacctgc tgctgctggt gctgatggct gcaattgtca tcaatctgcg gctgcagaat
    18541 tcgcgtctgg ggcgtgcctg ggtggcgatt cgcgaagacg aagttgctgc gcaagcgatg
    18601 ggaatcaata cgcgcaatgt caagttgctg gcatttgcga tgggtgcaag cttcggtggc
    18661 atcgccggcg gcatattcgc ggcaacgcaa ggcttcatca gtcccgagag tttcgttttt
    18721 gtggagtcga tcatggtgct ctccatggtc gtgctcggcg gaatgggcaa tgtatggggc
    18781 gtcgtgctgg gcgcaatttt gctttcgttc gtgccagaaa tattgcgcta caccgtagag
    18841 cccgcgcaaa aggccgtgtt cgggcgcctg ctgatcgagc ccgaagtgat cagaatgctc
    18901 ctgtttggtt tggctctggt gctgatgatg ctttaccgcc cggcagggct gtggccctcc
    18961 aagaggcgcg aacgcgagtt ctcggagacg gaccacggct gatgcagact gcgccattgc
    19021 tggcaattca tcgcatcgaa aagcgtttcg gcggattgat tgcgctggcc gacgtttcgt
    19081 taacgatcaa ccgtaacgaa atctatggtt tgatcggacc aaacggcgct ggcaaaacca
    19141 cgctgttcaa tgtgctgacc ggtctctatg tggcggagca gggcgcattc cagttcgatg
    19201 gcatggacct gatgggactc aagcctcatc gcgtggcgca atacggcatt gcgcgaacct
    19261 tccagaacat tcgcctgttc gggaacatga cagccatgga aaacgtaatg gtcggccggc
    19321 acgtgcgcac gcgcgcgggc gtcattggtg cgatattgcg cactagccgt gcccgcaagg
    19381 aggaagccgc gattcgcgct cgctcgcgag aactgttgcg ttacgttggc atcgcacggc
    19441 atgcaggcat gttgtcgaga aatctttcgt atggggatca gcgccggctg gagatcgcac
    19501 gcgcgttggc aacagaacca aagctgctag cactcgatga acctgctgcg ggaatgaacg
    19561 ctacggaaac cgtggaattg cgccagctac tggaaaccat tcgcaatgat ggtgtgacca
    19621 ttcttctaat cgagcatgac gtcaagctgg tgatgggcct ctgcgatcgc gtcgcggtcc
    19681 tggattatgg gcgcaagatt gccgaaggcg agcctctgca agtccaacgc aatccggatg
    19741 tcatccgcgc ttaccttggt ggagcgttgc cttgaacacg ctgcttgacg tcaaggcact
    19801 gaaagtggcg tatggcggaa tcaacgcggt caagggtgtg gacttgacgg tgggcgcggg
    19861 ggaaatggtt gcattgatcg gggcaaacgg cgccggcaag accacaacgt taaaagcgat
    19921 ttgcggattg ctgccgcacg caggggtggt aaagctgcgc ggtgaaaacg taaatagctg
    19981 ttcggcatat cagctggtcg caaggggtct tgttctggtt ccggaaggcc gcggtgtttt
    20041 tggccggttg actgtgcacg aaaatctaca gatcggcgcc tatatgcgcc gggactacgc
    20101 gacgattcgc tcagaccttg agcgcattta cggtctgttt ccgcaccttg cgcaaaggcg
    20161 tacccagact ggtggtaccc tttccggtgg agagcaacag atgctcgcca tcggccgcgc
    20221 gttgatgagt cggccgaagt tgctgttgct ggatgagccg agcatgggac tcgcgccgct
    20281 gatggtggaa aagattttcg atacagttcg gatggtgtcc cgggaaggcg ttacggtatt
    20341 gctggtggag caaaatgcaa agcttgcgct ggaagcaagc aaccgcggtt acgtaatgga
    20401 gagcggtctg attacactgt ccgatggagc gccggcactg ctcaacaatc cgcgcgtaag
    20461 gacagcctat ttgggcgagt gaagggttcg tggcgttgct tggccaactt gcgagggagt
    20521 acatgttcac cgaa
//
LOCUS       DATMZQ010000352         7528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3392778, whole genome shotgun sequence.
ACCESSION   DATMZQ010000352 DATMZQ010000000
VERSION     DATMZQ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392778"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VN496_11365"
     CDS             complement(<1..977)
                     /locus_tag="VN496_11365"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF17597.1"
                     /translation="MSAIAQSSPASLARDVDALFRRATDAGDVPGVVAMAIAGDSTLY
                     EGAFGTRRLGVAAAMDIDTVGWIASLTKPLTSVAAMQLVEQQRLQLDEPAGRWVRELA
                     HVQVLEGFDASGTPRLRAPRRPVTLRQLLTHTAGYGYGAWSAPLARYMELMNLPGTAT
                     CEHAALRLPLLFDPGERWNYGINIEWVGKIIEGASGMTLGAYLRENLFGPLGMDSSGF
                     RITPSMRKRLARLHERDAHGVLVAGDYEVPQSPEFELGGGGLYSTARDYVAFMRMILN
                     RGVGNGARILKAETVDLMCRNQIGNLRVERMVSTNAARSNDAEFFPGVP"
     gene            complement(974..1756)
                     /locus_tag="VN496_11370"
     CDS             complement(974..1756)
                     /locus_tag="VN496_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXF17598.1"
                     /translation="MSKGRLRVEREGPVGRLVLDNPERRNAIGAEMWRAMPKAIAEFH
                     ADSSIRCIVIRGEGIAAFAAGADISEFEGNRASESDVKEYDAATSAAHHAIETVPKPV
                     IALIHGFCVGGGLAVALSCDLRYADASSRFAVPAARLGLGYGIHGTGRLVATVGHAHA
                     REIMFSARRYDAQEALRIGLVNRVLPDNELDTYVARTVTELAANAPLTMAASKAVINA
                     LVEPRGDFSAAEASVAHCMNSQDYIEGRRAFMEKRAPSFKGK"
     gene            complement(1814..2989)
                     /locus_tag="VN496_11375"
     CDS             complement(1814..2989)
                     /locus_tag="VN496_11375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXF17599.1"
                     /translation="MDTEALSHLRVIDLTRVRAGPTCVKQFADWGADVIKIEMPAQDG
                     EDILGPRTGSDFHNLHRNKRSLTLNLKHPRGVEVLKRLAKISDVLVENYRPDVKHKLG
                     IDYDTLAKENPQLIYASISGFGQDGPYRDRAGFDQVAQGMGGLMWVTGLPGQGPVRAG
                     IPVADLSAGLFAALGILIALQERSRSGKGQWVQSSLLTSMIAMMDFQAARWLVEGDVP
                     AQAGNDHPTSMPTSVYPTEDGFVNIAAAGNPIFARMCQAFGDSALAKHADYVTAQLRS
                     RNRIALNAAIAVHTRRYPSAVLIELLAKAGVPCGPIYKLNEVFQDDQVKHLGMAVEVP
                     IPDGGEMTLVGPAIKLSRTPARMKRTVGPAGEHNHDILREIGYGDGDIAALRSANVI"
     gene            complement(3017..3871)
                     /locus_tag="VN496_11380"
     CDS             complement(3017..3871)
                     /locus_tag="VN496_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF17600.1"
                     /translation="MPFLTTDDGVKLHYEETGSGTPIVFVHEFAGDWRTWEPQLHHFS
                     RRYRCIAYNARGYPPSDVPADVDRYSQQRARDDIRAVLDALNIDRAHVVGNSMGGFAT
                     LHFGMTDSPRALSLVVAGCGYGAHPSLYAQFQEQSRELAKSMLQHGMAHVAATYGHGP
                     SRLQLRDKDPRGFAQFVRQFSEHSAQGSANTMLGYQARRPSLYDLAAEMARITAPTLI
                     IAGDEDDAVLEPSVMMKRTIPTAGLAVLPKSGHMTNLEDPALFNRLLEDFFHQVEAGR
                     WPTRILPS"
     gene            complement(3932..6145)
                     /locus_tag="VN496_11385"
     CDS             complement(3932..6145)
                     /locus_tag="VN496_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXF17601.1"
                     /translation="MCLGLFSACAHFSPGGATTPAKSAPQGVPYAGFSLGQDTEGNPV
                     ILDVIAGPAALAGLRPGDKVELVAGEKMDAARLLEIIHSASPGKRLPLRVLRDGRPLD
                     IDFIIGDWQQWATPAAYPPRVPFTEPPARSMPVWLDAIEAKVASMAPALMPERERLQK
                     MLGELSRQDTGYNSLLLSRQALADPGTLIAWEHRLVDILQPAGQAREHVVPVLCEILM
                     LDCSAIHSGLPVTATSAEESGLSAFAQNIAAANRRVREAFKTASPRDQLFRDVRYLLE
                     ETATRRTLIEQSDAMKGIRAMQASMQIDFAALLDAFNGVIIAASHVPDASQPTPRKIP
                     AALAAMVDGQILDFAEVDGGYIVVGGAGPNHYRMDLLYSVIDIGGDDQYTWGDGVPLE
                     TQIVIDVAGNDRYEARLGGPGAGWLGVSVLLDMAGDDLYVSTLGGCGAGAFGFGLLFD
                     AAGADTYRCDAWSTGAGIYGAGVLIDAGDDWDTYVSHSMSQGIGGPAGVGLLLDFGGD
                     DLYRANGPVQSSYGTPTTYMSFSQGVGFGIRPYDHGGFGALLDFAGNDRYEGGEFSQG
                     GGYFWGAGILHDSSGNDMYYGGRYTQGFAAHQAAGLFSDLAGDDVYWAMRAAAQGAAW
                     DQSVAMMFDGAGNDFYRAESLAQGAAAQQSSAWLFDAGGNDVYWSSGDSQGVAGDNSY
                     HHVAQDPVYSTGVLLDGGGDDRYSSGLANGEMRIRHTGSPANGRGNNGIAIDEQP"
     gene            6310..6996
                     /locus_tag="VN496_11390"
     CDS             6310..6996
                     /locus_tag="VN496_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HXF17602.1"
                     /translation="MKSNLRSKVDRAFLEKDGVRQAVRDAGHGHLLMSDHDVAASLAA
                     TLGLHPKEKPVWVFGYGSLIWNPLMEYAERRPARIHGFHRGFYLWSKINRGSPEIPGL
                     VLGLDKGGSCQGMAYRLHDDKLDSELALLWRREMLMGTYRPRWVSAELEGRTVRAIAF
                     VVDRKKPGYTGRLRDEQIVSIALKASGHYGSCADYLVQTAKSLEAAGIADQRLSRLAR
                     MVEARSARVG"
     gene            complement(7005..7397)
                     /locus_tag="VN496_11395"
     CDS             complement(7005..7397)
                     /locus_tag="VN496_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17603.1"
                     /translation="MFFTLWASQVNSARDEPSVGLPCPECGVIYDIREVTRERASARN
                     EPSNPAPVGPMIRFSPGDKADPRPHVDVLGSRSMREAMVEKYYVVVVRFDDNRWQRVE
                     LPDASGLKIGDRIHVHQNRIEPDDTEPR"
BASE COUNT         1518 a         2384 c         2151 g         1475 t
ORIGIN      
        1 ttcggcacgc cggggaaaaa ttctgcgtcg ttggagcgcg cagcattcgt gctcaccatg
       61 cgctccacgc gcaaattgcc gatctggtta cgacacatca ggtcaaccgt ttccgctttc
      121 agaatgcgtg cgccattgcc aacgccccga ttcaggatca tgcgcataaa ggccacataa
      181 tcccgtgcag tggaatacag tcctccacca cccagttcga attccggcga ctgcggcact
      241 tcgtagtctc ccgcgaccag gacgccatgt gcatcgcgct catgcaatcg cgccagacgc
      301 ttgcgcatcg atggcgtaat ccgaaagccg gagctgtcca tgccaagcgg cccgaacagg
      361 ttctcccgca aataagcgcc gagcgtcatg ccgctcgcgc cttcgatgat cttgccgacc
      421 cattcgatgt tgatgccgta gttccaacgc tcacccggat cgaacagcag cggcagccgc
      481 aatgcggcgt gctcgcaagt tgctgtgcca ggcaggttca tcaactccat gtagcgtgca
      541 agaggcgcac tccatgcgcc atatccataa ccggctgtat gggtcagcaa ctgccgtagc
      601 gtaaccggcc gtctcggggc tcgcaggcgc ggcgtccctg acgcgtcgaa accttccagt
      661 acctgcacgt gggcaagttc gcgcacccag cgcccggccg gttcatcaag ttggaggcgc
      721 tgctgctcca ccagctgcat ggctgcaacg ctggtaagcg gtttggtcaa cgacgcaatc
      781 catcccacgg tgtcgatgtc catggctgct gccaccccaa gccgacgggt accgaaagcg
      841 ccttcgtaca gcgtactgtc tccggcaatc gccattgcga ctacgccggg cacatcgccg
      901 gcatccgtcg cccgtcggaa cagagcatcg acgtcccgcg caagcgaagc tggactgctc
      961 tgcgcgatgg cgctcatttt cctttgaacg aaggagcgcg cttttccatg aatgctcttc
     1021 gcccttcaat gtagtcctgg ctgttcatgc aatgcgcaac cgatgcttcg gcggccgaga
     1081 aatcgccgcg cggttcgacc agtgcattga tgaccgcctt ggacgcagcc atggtcagcg
     1141 gcgcattggc ggccagttcc gttactgttc ttgccacgta agtgtccagt tcattatccg
     1201 gcaacacgcg gttgaccaaa ccgattctga gggcttcctg agcatcatag cggcgtgcac
     1261 tgaacatgat ttcgcgcgcg tgggcatgcc cgaccgtcgc gaccagccgg ccagttccat
     1321 gaatgccata accgagtccc agccgtgcgg ccggcaccgc gaagcggctg gatgcatccg
     1381 cgtaacgcag atcgcacgac agcgcgaccg ccagtccacc gccgacacaa aaaccatgga
     1441 tcaacgcgat caccggtttc ggcacggttt cgatggcgtg atgtgcggcc gaagtcgccg
     1501 catcgtattc cttcacgtca ctttcgctcg cgcggttgcc ttcaaattcg gaaatgtccg
     1561 ctccggcggc gaacgcagct atcccttcgc cgcggatcac gatgcaacga atggagctat
     1621 cggcgtgaaa ttctgcgatg gcttttggca tcgcccgcca catttccgcg ccgatcgcat
     1681 tgcgtcgttc gggattgtcc agtaccagac ggcctaccgg cccctcgcgt tccacgcgca
     1741 gcctgccctt gctcatgctt tctccttcgc ttctttgttt cgccgctcag gattcaatgc
     1801 gctgcgctgc tgctcagatc acgttcgcac tgcgcaacgc cgcgatatcc ccatcgccat
     1861 aaccgatctc gcgcaggatg tcgtggttgt gttcgcctgc gggaccaacc gtgcgcttca
     1921 tgcgcgccgg agtgcgggac agcttgattg cgggaccaac cagcgtcatc tccccgccgt
     1981 ccggaatagg tacctctacc gccatgccca gatgtttcac ctggtcatcc tggaacactt
     2041 cgttgagctt gtagatcggt ccgcacggta caccggcttt cgccagcaat tctatgagta
     2101 ccgccgaagg atagcgccgc gtatggacgg caattgccgc gttgagcgcg atgcgattgc
     2161 gcgaacgcaa ttgggctgtc acataatccg catgtttcgc caaagccgaa tcaccaaatg
     2221 cctggcacat gcgcgcgaat atcggattgc cggccgccgc gatattgacg aaaccgtcct
     2281 cggtcggata aacgctggtc ggcatcgacg tcggatggtc gttacccgcc tgtgccggca
     2341 cgtcaccctc gaccagccag cgcgcggcct ggaagtccat catcgcgatc atcgaagtca
     2401 acagcgaact ctgcacccac tggccttttc cggaacggct gcgctcctgc aaggcaatca
     2461 ggatgccgag cgcggcgaac aatcccgcgc tcaagtcagc caccgggatg ccggcacgca
     2521 cgggaccctg cccaggcaaa cccgtgaccc acatcagtcc gcccatgccc tgcgccacct
     2581 ggtcgaatcc cgcccgatcg cgataaggtc cgtcctggcc gaatcccgaa atgctggcat
     2641 agatcagctg cggattctct ttggcaagcg tgtcgtaatc gatgccgagc ttgtgcttca
     2701 cgtccgggcg ataattttcg accagcacat ccgagatctt cgcaagccgc ttgagaactt
     2761 ccacgccgcg tggatgtttt agattcagtg tcagcgagcg cttgttgcga tgcaggttat
     2821 gaaaatcgga gccagtgcgg ggaccgagaa tatcctcgcc gtcctgcgcc ggcatctcga
     2881 tcttgatgac atccgcgccc cagtccgcaa actgctttac gcatgttgga ccggcgcgca
     2941 cgcgcgttaa atcgataacg cgcagatggg agagagcttc agtgtccacg gcagcgctga
     3001 ttgatgaaaa ataatttcag ctcggcagga ttcgggttgg ccagcgaccc gcctcgacct
     3061 ggtggaagaa atcttccagc aatcgattga acaatgccgg atcttcgagg ttcgtcatat
     3121 gaccgctctt cggcaacacg gcgagtccgg ccgtcggaat agttcgtttc atcatcacgg
     3181 aaggctccag caccgcatcg tcttcatcac ccgcgatgat taaggtgggc gccgtaatgc
     3241 gcgccatttc agccgccagg tcgtaaaggg acgggcggcg cgcctggtag cccagcatgg
     3301 tattcgccga gccttgcgct gaatgttcgg aaaactggcg cacgaactgc gcgaaccccc
     3361 gaggatcctt gtctctaagc tgcagtcgcg atggaccatg cccgtatgtc gcagcaacgt
     3421 gcgccatgcc gtgctgaagc atggattttg caagttcccg ggattgctcc tgaaattgcg
     3481 catagaggga cggatgcgcg ccatagccgc aacccgcaac cacaagagac agcgcacgcg
     3541 gcgaatcggt catgccgaag tgcagcgtcg cgaaaccgcc catcgaattg ccgaccacat
     3601 gcgcgcgatc gatgttcaac gcatccagta ccgcgcggat atcgtctcgc gcacgctgct
     3661 gggaatagcg atcaacgtcg gccggcacat cggatggagg atagccgcgc gcgttatagg
     3721 caatgcagcg atagcggcgt gagaaatgat gcaattgcgg ttcccaggtg cgccagtcgc
     3781 ccgcaaactc gtgcacgaaa acgatcggcg tacctgaacc ggtctcctca tagtgcagct
     3841 tgacgccatc gtccgtggtc agaaaaggca tttgttagcg gtcctggctg aatggcgcag
     3901 tctagcgcac tgggccccca ggtccagaac gtcacggctg ttcatcgatg gcgattccat
     3961 tgttgccacg tccgtttgcc ggactgccgg tatgccggat gcgcatttct ccgttggcaa
     4021 gcccgcttga atagcgatcg tcaccaccgc catcgagcag cacgccagta ctgtacacag
     4081 ggtcctgcgc cacatggtga taggaattat caccggccac gccctgcgaa tcgccgctgg
     4141 accagtaaac gtcgttgccg cccgcgtcga acaaccaggc gcttgactgt tgtgccgccg
     4201 ctccctgcgc cagcgattcg gcacgataaa aatcattgcc tgcgccatcg aacatcatcg
     4261 ctactgactg atcccaggct gcgccctgcg cagccgcacg catggcccaa taaacatcgt
     4321 cgcccgcgag atcggaaaac agtccggcag cctgatgtgc ggcaaagcct tgcgtatagc
     4381 gtccgccata gtacatatcg ttgccacttg aatcgtgcag gatgcctgcg ccccagaaat
     4441 agccgccgcc ttggctgaat tcccctccct cgtagcgatc gttgccggcg aagtccagca
     4501 aggcgccgaa tccaccgtgg tcgtacgggc ggatgccgaa gcccacaccc tggctgaaac
     4561 tcatgtatgt cgtcggtgtg ccataagaag actgcaccgg tccgttggcc cggtacagat
     4621 catcgccgcc aaaatccagc agcagtccaa ctcccgctgg cccgccgatg ccctgactca
     4681 tgctgtgcga cacataggtg tcccagtcgt cgccggcatc gatcagcact cccgctccgt
     4741 aaatgcccgc gccggtcgac caggcatcgc agcgataagt atccgcaccc gccgcatcga
     4801 acaacaagcc aaagccaaag gcgccggcac cgcaaccacc gagcgtgctg acgtacaggt
     4861 cgtcccccgc catatccagc aacaccgaca ccccgagcca cccggcgccc ggcccgccaa
     4921 gccgtgcttc gtagcgatcg ttgccggcta cgtcgatcac gatctgcgtt tccagcggaa
     4981 cgccgtcacc ccaggtgtat tgatcatctc ctccaatatc gatgaccgaa tacagcaggt
     5041 ccatgcggta atgatttggg cctgctccac caacgacgat atatccgcca tcgacttcag
     5101 caaagtccaa aatttggccg tcgaccattg ctgccaaggc cgccggaatt ttgcgtggtg
     5161 tgggctggga agcatccggc acatgtgacg cggcgatgat taccccattg aacgcgtcca
     5221 gcagcgccgc aaagtcgatc tgcatgctgg cctgcatggc acgaatgccc ttcattgcgt
     5281 ccgattgctc gatcaaggtg cgcctggtcg ccgtctcttc cagcagatag cgcacgtcac
     5341 gaaacaattg atcgcgcggg gacgctgtct tgaacgcttc tcgcacccgc cggttcgctg
     5401 ccgcaatatt ttgagcaaat gcgctgagtc cgctttcttc tgccgaagtg gcagtgaccg
     5461 gcaaaccgga atgaatcgcg ctgcaatcga gcatcaggat ttcacatagc acaggcacga
     5521 cgtgttcgcg cgcctgccct gccggttgca atatgtccac gagccgatgt tcccatgcga
     5581 tgagcgtgcc gggatcggcc agcgcctgtc ggctcagaag cagggagttg tatcccgtgt
     5641 cctgccgcga aagctcgccc agcattttct gcagacgttc gcgttccggc atcagtgcgg
     5701 gagccatgga cgcgactttc gcttcgatgg cgtccagcca gactggcatg ctgcgcgcag
     5761 gcggttcggt aaagggcacg cgcggcggat aggcggctgg tgtggcccac tgctgccagt
     5821 ctccaatgat gaaatcgata tccaatgggc ggccgtcacg cagcacgcgc aacggtagtc
     5881 gcttgccggg cgatgcagaa tggatgattt ccagcaggcg cgctgcatcc atcttttcgc
     5941 ctgccaccaa ttcaactttg tctcccggcc gcaggccagc cagtgcagca ggcccggcaa
     6001 tgacatccag gatcacgggg ttgccctcgg tgtcctggcc gagcgagaac ccggcatacg
     6061 gcacaccttg cggtgcggac ttggcaggtg tcgttgcgcc gcctgggctg aaatgcgcgc
     6121 aggccgaaaa taaccccaga cacacggcgc acacggctag ctgaagcaac cgcgccgcgc
     6181 gacaccttcg cgccggttca atgctcgctg ctgctttcgg tctggtcgcg cgcgggctgg
     6241 ctggcatggg cacaaacatt agcagatgtt gttgcggcca gcgcctgtgg caacatgcag
     6301 actgtgacaa tgaagtccaa tctgcgatcg aaagtcgatc gcgcctttct ggagaaagac
     6361 ggtgtgcgcc aggcggtgcg ggatgccgga catgggcatc tgctcatgtc cgaccatgac
     6421 gtggctgcct cgctcgccgc tacgctcggg ttgcatccta aagaaaagcc ggtctgggta
     6481 ttcggctatg gctcgctgat ctggaacccg ctgatggaat acgccgaacg caggccggcg
     6541 cgcattcacg ggtttcatcg cggcttttat ctgtggtcga aaatcaatcg cggctcgccg
     6601 gaaatacctg gactggtgct gggactcgat aaaggcggct catgccaggg tatggcttat
     6661 cgactgcatg atgacaagtt ggacagcgaa ctggcattgc tgtggcgccg ggaaatgttg
     6721 atgggcactt atcggccacg ctgggtcagc gccgagcttg aaggccggac cgtgcgcgcg
     6781 attgcgtttg tggtggaccg caaaaagccc ggctacaccg gccggttacg cgatgaacag
     6841 atcgtctcca ttgcgcttaa agcctcgggt cactatggct cttgcgcgga ttatttggtg
     6901 cagacggcaa aaagtctgga ggccgctggc atcgcggatc agcggctatc gcgactggcc
     6961 agaatggtag aagccagaag cgcccgcgtg ggatgaaagt ggtcttagcg cggctccgtg
     7021 tcatccggtt caatccggtt ctgatgaacg tgaatgcggt cgccgatctt caacccgctt
     7081 gcatcaggga gttccacgcg ctgccaacga ttgtcgtcaa aacgcacgac cacgacatag
     7141 tacttctcca ccatggcttc gcgcatggac cggctgccga gcacatcgac atgcgggcgc
     7201 ggatcggctt tgtcgccagg cgaaaaacga atcatcggtc cgaccggcgc cggattgctc
     7261 ggctcgttgc gtgccgaggc ccgctcgcgc gtgacttccc tgatgtcata gatcacgccg
     7321 cattccgggc acggaaggcc caccgagggc tcgtcgcgtg cgctgttgac ttgactcgcc
     7381 cataaggtga aaaacatcag cgcgacaaac ttgaggaagg caatacccgg gctgcgagcg
     7441 gtatgcatag gactaaagcc agtgaaccgg aatcaacttt gacctgtaca caataaaaga
     7501 ctggcagcac atccgccagt accaaata
//
LOCUS       DATMZQ010000353         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3048726, whole genome shotgun sequence.
ACCESSION   DATMZQ010000353 DATMZQ010000000
VERSION     DATMZQ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048726"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="lnt"
                     /locus_tag="VN496_11400"
     CDS             <1..896
                     /gene="lnt"
                     /locus_tag="VN496_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXF17604.1"
                     /translation="AGALLKRHQWTQPLPGEPTTVTLLQGNIPQELKWRPERAIATLQ
                     TYLELVRSTDSKLILLPETALPMLNTDIPPDYLRALTDRTRLNGGDMLFGVPEQDASG
                     RYYNSVMSLGTEPAQVYRKHHLVPFGDFFPLQPVLGWIMRLLHIPMSDFSHGPAVQRP
                     IQVAGQKVAVNICYEDAFGEEIIRQLPEATVLANFTNDAWWGDTIASRQHLQIAQMRA
                     LETGRPMLRATNTGVTAIIDPTGHIVATAPEFTTTKLSGEVRGYQGSTPYVAIGNAGV
                     LLLAGLMVLVQWLIARVRHLR"
     gene            complement(916..1398)
                     /locus_tag="VN496_11405"
     CDS             complement(916..1398)
                     /locus_tag="VN496_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HXF17605.1"
                     /translation="MMFRRVAVIVALCVAAVSMHASAESSAPWGQAKSVEKTYSKQKV
                     VYDVSVKTVAQLENVLDRASHLSVLNDADPFDSKIVIVLHGDEIGFFAIANFAKYRDL
                     MTRAQSLTVGGTIDIRMCRIAARRRGFDARDIHGFVTMVPMADAEIVDLQKQGFAYMQ
                     "
     gene            1491..2435
                     /gene="glyQ"
                     /locus_tag="VN496_11410"
     CDS             1491..2435
                     /gene="glyQ"
                     /locus_tag="VN496_11410"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXF17606.1"
                     /translation="MLNFQEVILALQRYWSERGCAILQPYDMEVGAGTSHTATFLRAI
                     GPEPWRAAYVQPSRRPKDGRYGENPNRMQHYYQFQVVLKPSPLDIIDLYLGSLRALGI
                     DPQAHDIRFVEDDWENPTLGAWGLGWEVWLDGMEVTQFTYFQQVGGLDCKPITGEITY
                     GIERLAMYLQNVENVFDLVWTSGLTYRDVYQQNEVEQSTYNFEQSNTAILFQHFNDYE
                     AEATRLMAAQLALPAYEMVLKAAHTFNLLDARGAISVTERATYIGRIRNLARSVSQAY
                     FDSRLRSGFPMSKPEQLQRLGVDVPAMKAALLEKERAA"
     gene            2432..>2838
                     /locus_tag="VN496_11415"
     CDS             2432..>2838
                     /locus_tag="VN496_11415"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXF17607.1"
                     /translation="MSANLVVELFTEELPPKALKRLGEAFAGGIAESLRQSGLLDATS
                     KAKSFATPRRLAVYLTSVLPKGSDRPLEQKLMPVAVARKQDGTWSDALRKKLAAMGHE
                     GLANAPVNSTVGADSLAIKPDGKANSVYLRTVSL"
BASE COUNT          626 a          813 c          796 g          603 t
ORIGIN      
        1 tcgcgggagc ccttctcaaa cgccaccaat ggacccaacc gctgccgggg gagccgacca
       61 ctgtgacgct gctgcaagga aatattccgc aggaactcaa atggcggccg gaacgcgcaa
      121 tcgccacgct gcaaacctat ctggagttag tacgctcgac cgacagcaag ttgattctgt
      181 tgccagagac tgcactgccc atgctcaata ccgacatacc gcccgactat ctcagggcgt
      241 taaccgaccg tacaagactg aacggcggcg acatgctgtt tggtgttccg gaacaggatg
      301 cgtccggacg ctattacaac agcgtcatga gcttgggcac agagccggct caggtctacc
      361 gcaagcacca tcttgtgccc ttcggcgatt tttttcctct gcaaccggtg ctgggctgga
      421 tcatgcggct acttcacatt ccgatgtcgg acttttctca cggtcccgcc gtgcagagac
      481 cgatacaggt ggcgggtcag aaagttgcgg tgaatatttg ctacgaggac gcgttcggag
      541 aggagatcat ccgccagttg cctgaagcca ccgtgctggc aaacttcacc aatgacgcct
      601 ggtggggtga cacgatcgct tcccggcaac atttgcagat cgcgcaaatg cgcgcattgg
      661 aaacgggccg gccaatgttg cgagcaacta acaccggcgt gacggccatc atcgatccga
      721 ctgggcacat cgtcgcaacg gccccggaat ttaccacgac gaagctaagc ggcgaggtgc
      781 ggggctatca gggcagcacg ccttacgtcg caatcggcaa tgcgggcgtt ttgctgctgg
      841 caggcctcat ggtgctggtc cagtggctta ttgcgcgcgt gcggcatttg cgctaatcca
      901 atcgcggccg ctctttcact gcatgtaggc aaagccctgc ttttgcaggt cgacaatttc
      961 cgcatccgcc atcggtacca tggtcacgaa gccgtggatg tcacgcgcat caaagccccg
     1021 gcgccgtgcc gcaatcctgc acatgcgaat atcgatggtg ccgccaaccg taagactttg
     1081 cgcccgcgtc atcaggtccc tgtactttgc gaaattggca attgcaaaaa aaccaatctc
     1141 gtctccatgc agcacaataa caatcttgct gtcgaatgga tccgcatcgt tcagcacgga
     1201 caagtgactg gctctgtcga gtacgttttc cagctgtgcc acggttttaa ccgatacgtc
     1261 gtacacgact ttttgcttgc tgtaggtttt ttccacgctt ttcgcttgtc cccaaggcgc
     1321 ggaactctct gcggaagcat gcatggaaac ggcggccacg cacagtgcaa caataacagc
     1381 gacccgtcgg aacatcattg cttcatcctc gttaaaacag acggttttga gtacaattct
     1441 gcctttcttt cgggacgttc aagtcctgaa ttcgtggctg aatttcatcg atgttgaatt
     1501 ttcaggaagt cattctagcg ctgcagcgat actggagtga gcgcggatgc gccatcctgc
     1561 aaccttacga catggaagtc ggtgcaggaa cttcgcacac cgcgacgttt ctgcgggcca
     1621 ttggaccgga accatggcgt gctgcatacg tgcagccatc acgccgtccg aaggacggcc
     1681 gctatggcga aaatccaaat cggatgcagc actactacca gtttcaggta gtactcaaac
     1741 cctctccgct cgatatcatc gatctgtatc tgggatcgct gcgcgcgctc ggcatcgatc
     1801 cgcaggcgca tgatattcgc tttgtggagg acgactggga gaatccgacc cttggcgcct
     1861 ggggcctggg ctgggaagta tggctcgatg gcatggaagt cacccagttc acttatttcc
     1921 agcaggtagg cgggctggat tgcaagccga tcacaggtga aatcacttac ggcatcgaac
     1981 gccttgcgat gtatctgcaa aacgtggaaa acgtgttcga tctggtttgg acatcagggc
     2041 taacgtatcg cgacgtgtat cagcagaatg aggtcgagca gtcgacttac aacttcgaac
     2101 agtcgaacac ggcgatcctg ttccagcatt tcaatgatta cgaggccgag gcgacgcgcc
     2161 tcatggctgc gcaactcgcg ctgcctgctt acgaaatggt cttgaaagcc gcgcatacgt
     2221 tcaatttgct cgatgcgcgg ggcgcgattt ctgtcaccga gcgcgcaacg tacatcggcc
     2281 gtatacgcaa tcttgcgcgc agcgtgtccc aggcttattt cgattcccgg ctgcgcagcg
     2341 gatttcccat gagcaagcca gagcaactgc aaaggctcgg tgttgacgtt ccggcaatga
     2401 aagcggctct gctggaaaaa gaacgggcag catgagcgcc aacctggtgg tcgaactatt
     2461 caccgaagaa cttcctccga aagccctgaa gcgccttggc gaagcatttg caggtggaat
     2521 agccgagagt ctgagacaaa gcggtttact ggacgcgact tcgaaagcga aatcctttgc
     2581 cacgccacgc cgcctggcgg tgtacctgac atccgtcctg ccgaagggct cggaccggcc
     2641 gctggaacaa aaactcatgc cggtcgcggt tgcgcgcaag caggatggaa cctggagcga
     2701 tgcgctacgc aaaaagcttg cggccatggg acacgagggg ctggcgaatg cgccggtcaa
     2761 ttccaccgtt ggggctgact cgcttgccat caagccggac ggcaaggcca acagcgttta
     2821 tttacggacg gtctccct
//
LOCUS       DATMZQ010000354         4766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2014999, whole genome shotgun sequence.
ACCESSION   DATMZQ010000354 DATMZQ010000000
VERSION     DATMZQ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014999"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3775)
                     /gene="hrpA"
                     /locus_tag="VN496_11420"
     CDS             complement(<1..3775)
                     /gene="hrpA"
                     /locus_tag="VN496_11420"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF008348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HXF17608.1"
                     /translation="MLRERASLRGRLRRLEDGLRRGKDVSVELARLLADAKSSEAQVR
                     ERQEKLPRVTYPEELPISAKRAEIAQAVKHHQVVIVCGETGSGKTTQLPKICLELGRG
                     LHGMIGHTQPRRIAARSVAARVAAELHTPLGQAVGFQVRFTDKTSADNYIKVMTDGIL
                     LAQTQTDRWLSQYDTLIIDEAHERSLNIDFLLGYIKQLLPRRPELKLIITSATIDAGR
                     FSAHFGNAPIIEVSGRLYPVDIWYRPFDEDDEDEHEIDLPEAIVHAVDEVTARTTSGD
                     VLVFLPGEREIRDAAEALRKQHPQGIEILPLFARLSAQDQERIFAPGSAARRIVLATN
                     VAETSLTVPGIRFVVDSGLARINRYSYRNKVELLQIEKISRASANQRAGRCGRVMNGI
                     CVRLYGEEDFGARTEFTDPEILRSSLASVILRMGALKLGDIEDFPFVDAPASRMIADG
                     YQLLTELGAVDEARTLTPVGQQLAKLPIDPRVARMLVAAKQEGCLKEVLIIAAALSVQ
                     DPRERPMDKQEAVTQAHAEFVDERSDFLAYISIWKWFEDALVHKKSNRRLVLDCHERF
                     LSYLRLREWRELHEQLNALIGEMGWRLNEKESRYEEIHRALLAGLLGNIGFKSEEAGI
                     YLGARQIKFVLSQGSGLKKKGPKWVMAAELTETTRLFARCVAAIEPEWIERLGGHLAK
                     RSYREPHWSKDSGSVIAYEQVTVYGLVVVPKRRVQYGPIEPAKARELFIRGALVAGHY
                     PGQPEFLLHNRALIEDVQQLEHKSRRHDVLVDDEAIFAFYAERIPEGIYSTVDFEKWR
                     RTAEGSDPKLLFLSRDYLMRHAAEQITEELFPDALTVGMQTFDLSYRFDPGHALDGVT
                     MTVPLHLLNQVDERRCEWLVPGLLRDKITYLVKGLPKGLRKHLVPVPQVVTACMEVLE
                     PDSTSLTAALSQALFKKTGVDIPLDAWDIGDLPPHLLMNFRVVDENEHEIASGRDLAA
                     LRNQLGVKARRTFSETASSAFERKGLTTWDLDELPEQVQVNRGRGKLIGYPAIVDEGK
                     TVGLSLLDTEAEADAATRRGLRRLFQLALPEHIKSLARSLPGFNEMQLRYALLDEQPG
                     GRPDKSALADRLRDELVTAICDRAFFVEHEPVRNRKQFDTRVAKAKTRLMDVAQEICR
                     IAGEILVEHQSLRAKLNQPQYSAWPRALADIRAQLRELLPPGFLANVAFDRLKHYPRY
                     LRAVAMRLDKIASNPERDANWQQQLARYWQAYQTRLVDDRARGVRDPKLEDMRW"
     gene            complement(4043..>4766)
                     /locus_tag="VN496_11425"
     CDS             complement(4043..>4766)
                     /locus_tag="VN496_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXF17609.1"
                     /translation="LPFSPSLDQPGVFARSVADAAFLASNLTAQRTVITPEVRPLRNA
                     PKLAAVRSPVWYLAQPEQRLQFESDIGRLRDAGASVDVLEISGAFDGAQRIHRTIMLY
                     EAARVAKSAREIWRDGFSDYLNAALDEGENIREPDYRDALKIRTQLQQSLAEFFDRGY
                     SAIMTPPAAGEAPPTLDNTGDPRFCTIWTLIGVPAITIPTGKGPHGLPLGLQLVGAAE
                     EENYLLATAAWCERYSPFTGLV"
BASE COUNT          980 a         1484 c         1323 g          979 t
ORIGIN      
        1 tccagcgcat gtcttccagt ttcggatcgc gcacgccgcg cgcgcggtcg tccaccagac
       61 gcgtctgata tgcttgccag tagcgagcca gttgttgctg ccagttggca tcgcgttccg
      121 gattggatgc gatcttgtcc aatcgcatcg cgactgcgcg caaataacgc ggataatgct
      181 tcagccggtc gaacgccaca ttcgccagaa atccgggcgg cagcagctcg cgcagttggg
      241 cgcggatatc ggccagcgcg cgtggccatg ccgaatattg cggctgattt aatttcgcgc
      301 gcaagctttg atgttcaacc agaatttctc cggcgatgcg gcaaatctcc tgcgcgacgt
      361 ccatcagacg tgtcttggcc tttgcgacgc gcgtatcaaa ctgcttgcgg ttgcgcactg
      421 gttcgtgctc gacaaaaaat gcccgatcgc aaatggctgt caccagttcg tcgcgcagcc
      481 gatcagccag cgccgacttg tccggccggc cgccgggttg ttcatcgagc aacgcgtaac
      541 gcagctgcat ctcattgaat cccggcaggc tgcgtgcaag cgatttgatg tgttccggca
      601 aggcgagctg gaacagccgg cgcaatcccc ggcgcgtcgc ggcatcggcc tccgcttccg
      661 tatccagcaa tgacagaccc accgtcttgc cctcgtccac tatggccgga taaccgatca
      721 acttgcctct gccgcgattg acttgcacct gctcgggcag ttcgtcgaga tcccaggtcg
      781 ttaaaccttt gcgctcaaaa gctgagcttg cggtttcgct gaatgtccgg cgcgctttca
      841 cgcccagctg attgcgcagc gccgccaaat cccggccgct tgcgatctcg tgttcgtttt
      901 catcaaccac acggaaattc atcagcagat gcggcggtag atccccgata tcccaggcgt
      961 ccagaggaat gtctacgccg gtctttttga atagtgcctg cgacaaggcc gcggtcagcg
     1021 aagtactgtc aggctccaga acttccatgc aggcagtcac cacctgcggc acgggcacaa
     1081 gatgtttgcg caagcccttg ggcaagccct ttaccaggta ggtgatcttg tcgcgcagta
     1141 aaccaggtac caaccattcg caacggcgtt cgtccacctg attcaataaa tgcagcggca
     1201 cggtcatggt tacaccgtcc agggcatggc ctggatcgaa tcgataggac aggtcgaagg
     1261 tttgcatgcc caccgtaaga gcatccggaa atagctcctc ggtgatctgc tcggcagcgt
     1321 gacgcatcag ataatcgcgg ctcaggaaca gcagtttcgg atcgctgccc tcagccgtac
     1381 gccgccattt ttcgaaatcc acagtgctgt agatgccctc gggaatgcgt tcggcataga
     1441 aggcgaatat cgcttcatcg tcgaccagca catcgtggcg ccgggattta tgctccagtt
     1501 gctgcacgtc ttcaatgaga gcccgattgt gcagcagaaa ttccggctgc cccggatagt
     1561 gacccgccac cagagcgccg cggatgaata actcacgcgc ttttgccggt tcgatcggac
     1621 catactgcac gcgccgtttc ggcaccacca ccaggccgta tacggtgact tgctcgtagg
     1681 caatcacaga accgctgtcc tttgaccagt gtggctcgcg atagctgcgc ttcgcgagat
     1741 gcccacccag acgctcaatc cattccggct cgatggccgc cacacatcgg gcaaacaagc
     1801 gcgtggtttc cgtcagttca gctgccatga cccacttggg acctttcttc ttcagcccgg
     1861 agccctggct cagcacgaac ttgatctggc gcgcgcccag ataaatgccg gcctcttcgg
     1921 atttgaagcc gatgttcccg agcaagcctg ccagcaaggc acgatgaatt tcctcatagc
     1981 gcgactcttt ttcgttcagc cgccatccca tttcgccaat cagcgcgttt agctgctcgt
     2041 gtagttcccg ccactcccgc agccgcaggt aggaaagaaa acgctcatgg cagtccagca
     2101 ccagcctgcg attggacttt ttgtgaacca gtgcgtcttc gaaccatttc cagatgctta
     2161 tataggccaa gaaatcggag cgctcatcga cgaattcggc atgcgcctgg gtaacagcct
     2221 cctgcttgtc cattggccgc tcgcgcggat cctgcaccga cagtgcggcc gcgatgatca
     2281 atacttcctt caaacagcct tcctgcttcg cagctaccag catgcgtgca acgcgcgggt
     2341 caatcggcag tttggctagt tgctgcccca ccggggtcag cgttcgcgct tcatcgacgg
     2401 caccaagttc agtcaacagc tggtagccat cagcaatcat gcgggaagcc ggcgcatcga
     2461 caaacggaaa atcttcgatg tcgcccaact tcaacgcgcc catgcgcagg atgaccgacg
     2521 caagcgaaga gcgcaggatt tccggatcgg taaactccgt gcgtgcgccg aagtcttctt
     2581 cgccatacag tcgcacgcaa attccgttca tgacgcggcc gcaacgcccg gcgcgctgat
     2641 tggccgaggc ccgcgaaatc ttttctatct gcagcaattc gaccttgttg cggtagctgt
     2701 agcgattgat gcgtgccagt cccgagtcca ccacaaaacg gatcccggga acggtcagtg
     2761 aagtttccgc cacattggtg gcgagcacga tgcgccgtgc cgcactgccg ggtgcaaaaa
     2821 tacgctcctg gtcctgcgcg gataatcttg caaacagcgg caggatctct atcccctgcg
     2881 gatgctgctt gcgcaacgct tcggctgcat cacggatttc gcgctcgcca ggcagaaaca
     2941 ccagcacgtc acccgaagtt gttctggcgg tcacctcgtc gacggcatga acgatggcct
     3001 ccgggaggtc aatctcgtgc tcgtcctcgt cgtcttcatc aaaaggccga taccagatgt
     3061 ccaccggata caggcggccg gacacttcga tgatcggcgc attgccaaaa tgtgcagaga
     3121 accgccccgc atcaatagtg gcggaggtga taatgagctt cagttccgga cgccgcggca
     3181 ataattgttt gatatagccc aacagaaaat cgatgttcag actccgctcg tgtgcctcgt
     3241 cgataatcag ggtgtcgtac tgcgacagcc agcgatcggt ctgtgtctgg gcaagcagga
     3301 tgccgtcggt catgactttg atgtagttat cggcactagt cttgtcagtg aaacgaactt
     3361 ggaaaccaac tgcttggccc aacggggtat gcaactccgc ggcgacccgt gcggcgacgg
     3421 acctcgcagc aatccggcgc ggttgcgtgt gcccgatcat gccgtgcaga ccgcggccca
     3481 gctccaggca gatttttggc aattgcgtcg tcttaccaga cccggtctca ccgcatacaa
     3541 ttaccacctg gtggtgtttc accgcctgcg cgatctccgc gcgcttggcg ctgatgggca
     3601 attcttccgg atacgtcacc cggggcaatt tttcctggcg ctcacgcacc tgggcttctg
     3661 agctttttgc gtcggctagc agtcttgcga gttccaccga cacatctttg cctcgacgca
     3721 agccatcttc cagccgtcta aggcggccac gcaaagaagc ccgctcgcgc agcatgcttc
     3781 gttcaacaca tgcgcggaga gcctgccaat cgactatggc gggagaaaaa tcaggcggga
     3841 ccggattgga attcatacgg gacacgcaat agcaaccaga acccaaagcg ggaaaaaaat
     3901 ggtaacggac ccatgccgtg tttcaggcgc cgagcaggtt cgatcagcag aacagtccaa
     3961 ggtttctggc gcacccggct atccgagata cgcggtcgtg ctggcggcca gccagccgcc
     4021 tccatgccgc tgggaatcgc aactagacca gcccggtgaa tggtgaataa cgctcgcacc
     4081 acgctgccgt tgcaagcagg tagttctctt cttccgccgc gccgaccagc tgcagtccaa
     4141 gcggcagacc gtgcggcccc ttgcccgtcg gaatagtaat ggccggaacg ccgatcagtg
     4201 tccagatggt gcaaaagcgc ggatcgccag tattgtccaa ggtagggggc gcttcccccg
     4261 cggccggcgg cgtcatgatg gccgaatagc cacgatcgaa gaactccgca aggctctgct
     4321 gcaattgcgt gcgtattttc agagcgtcac ggtagtccgg ctcacggatg ttctcgcctt
     4381 catcgagtgc cgcgttcagg taatccgaga aaccgtcgcg ccagatctca cgcgcactct
     4441 tcgccacccg ggccgcttca tacagcatga tcgtgcgatg gattctttgc gcgccatcga
     4501 atgcgccgct gatttccagc acatcaaccg acgcgcctgc gtcgcgcaga cggccgatgt
     4561 cgctttcgaa ctgcaaacgc tgctccggct gggccagata ccagaccgga gagcgcaccg
     4621 cggctaactt cggcgcatta cgcaaaggcc tcacttccgg cgtaatgacc gtacgctgcg
     4681 cagtgagatt cgaagcaaga aaagctgcat cggccacgga gcgtgcgaac acacccggct
     4741 gatccaagct tggtgaaaat ggcagg
//
LOCUS       DATMZQ010000355         6409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4085621, whole genome shotgun sequence.
ACCESSION   DATMZQ010000355 DATMZQ010000000
VERSION     DATMZQ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4085621"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VN496_11430"
     CDS             <1..418
                     /locus_tag="VN496_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17610.1"
                     /translation="TPVPQLPPPSSARHVPIEVRHWHQDERDLGKRNGRDPITVVILS
                     MARFDAMGVDQNSLTFGAKGNEKSLFRCRKEAIDINRDRRPDLVCYFKPDVANFQPGD
                     LNGVLKGKTKKGEMIEGTAALKIFSRKHRRFYHHHK"
     gene            complement(501..2036)
                     /gene="murJ"
                     /locus_tag="VN496_11435"
     CDS             complement(501..2036)
                     /gene="murJ"
                     /locus_tag="VN496_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXF17611.1"
                     /translation="MNLLKALATISSMTLVSRILGFVRDAVIARLFGAGLYTDAFFVA
                     FRLPNLLRRLFAEGAFAQAFVPILGEYRAARTVDETRALIDHVATLLAAALIVVSAVG
                     IVAAPLIIYVTAPGFASSADKFAITVQLLRITFPYIFFISLTSLAGGILNTWSRFSVP
                     AFTPTLLNVSFIVFALWLAPYFDPPVLALAWAVFAGGVLQLAFQLPFLARIGMLPRVR
                     FNLRDPGVWRIVTLMGPAVFGVSIGQISLLINTIFASFLVTGSVSWLYYADRLMEFPT
                     GLLGVALGTILLPSLTRSFADHTGEEYSKLLDWGLRLTFLLALPCAVALALLAVPLIA
                     TLFYHGAFNGQDVLMTRNALVAYSVGLLGLILVKVLAPGFYARQNIRTPVKIAIITLV
                     ATQLMNLAFIGPLRHAGLALAISLGACINAGMLFYQLRKHNIFKPQKGWFEFLLKVGL
                     ALVLMGAALKWAAGAESAWLTDTLQWRVTRLTGLVFLGIAIYFGSLWLFGFRLRDFAK
                     RAV"
     gene            2226..2489
                     /gene="rpsT"
                     /locus_tag="VN496_11440"
     CDS             2226..2489
                     /gene="rpsT"
                     /locus_tag="VN496_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXF17612.1"
                     /translation="MANIASAKKRARQAEKRRAHNVGQRTEVRSAIKNVKKAIAAGDK
                     KAAQAQFQLSQSRIDSIADKRIVHKNTVSRQKSRLAAAIKAMS"
     gene            complement(2566..2892)
                     /locus_tag="VN496_11445"
     CDS             complement(2566..2892)
                     /locus_tag="VN496_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HXF17613.1"
                     /translation="MSGPPIVELIIVGLKGNGEIFQPGDWAERLCGCMSLFGDSQRNS
                     YSPYTKPVSASGVNCLVVDRRLEHMSPEAFQFLMTFCRDNELQIREGRQEIRQSLQVQ
                     KPPKVA"
     gene            3293..4486
                     /locus_tag="VN496_11450"
     CDS             3293..4486
                     /locus_tag="VN496_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXF17614.1"
                     /translation="MMQHVMKTYARQRVAFSRGNGPWLWDLQGNRYLDCVSGVAVNGL
                     GHAHPRLVEAVSAQAARAMHVSNLYEIPEQEELAARLCALAGMDSAFFCNSGCEANEA
                     AIKLARLFGHQQGIESPAIVVMENAFHGRTIATLSATGSRKVQAGFEPLLTGFVRVPY
                     NDVAALMQVAEVNPNIVAVLVEPVQGESGIQIPEDNYIPRLRKICDEHGWLLMLDEVQ
                     TGVARTGRWFAHQHLDVKPDVMSLAKGLGNGFPIGACLARGPAATVFTPGKHGSTFGG
                     NPLACVCALTTLSIIEDEDLVQRVAKIGQQILDGIAKNLAGAPGVKQVRGKGLMIGIE
                     LDRPCADLVTQGLENGLVINVTAENVVRLLPPLNLQDSEVLLLIDGVSRLIRQFSHAS
                     VALAS"
     gene            4494..5420
                     /gene="argF"
                     /locus_tag="VN496_11455"
     CDS             4494..5420
                     /gene="argF"
                     /locus_tag="VN496_11455"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXF17615.1"
                     /translation="MQTPRHFLQFRDFSRDEFAHLFERTQWIKQQFKSYKTYRPLFDR
                     TLVMIFEKQSTRTRLSFEAGMHQLGGSAIFLSTRDSQLGRGEPVEDAAQVISRMCDVV
                     MMRTFDQEIIKRFADHSRVPVVNGLTNEYHPCQILADIHTYIEHRGPIQGKTVAWIGD
                     SNNVCNTWLQAAEVLDFNVHVSTPPGYEVEPERAGLYGTDHYEEFTDPMEAVRGAHLV
                     TTDVWTSMGFEAENEERKRDFEQWQVDAEMMKGAASDALFMHCLPAHRGEEVAAAVID
                     GPQSVVWDEAENRLHTQKALMEYLVLGKVASI"
     gene            5585..6097
                     /locus_tag="VN496_11460"
     CDS             5585..6097
                     /locus_tag="VN496_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17616.1"
                     /translation="MIRLSLSSIALVLSLAIPGALFGADDDVSWIADKNGCKVANPFP
                     QPGETITWNGQCKDGKADGEGLLQWYVGGKLADRYEGSLKEGWAEGKGTLTRTEGGRY
                     SGEWKHSLQDGNGRYEASDGSWYEGQWKDGQPHGHGQYRTPDGRTVVGEWVNGVYEGD
                     MDSDDNPNKT"
     gene            6113..>6409
                     /locus_tag="VN496_11465"
     CDS             6113..>6409
                     /locus_tag="VN496_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HXF17617.1"
                     /translation="MKKKNEEKPIKKVVLAYSGGLDTSVILKWLQDTYHCEVVTFTAD
                     IGQGGEVEPARRKAKKFGVSQIYVEDLREEFVRDFVFPMFRANALYEGEYLLGTS"
BASE COUNT         1629 a         1694 c         1820 g         1266 t
ORIGIN      
        1 gactcctgtg ccgcagctac caccgccttc ttcggccagg catgtgccga tcgaagtcag
       61 gcactggcat caggacgagc gtgaccttgg caagcgcaat ggtcgggacc ccattacggt
      121 cgtcatattg tcgatggctc gctttgacgc gatgggagtg gatcagaact cactgacgtt
      181 cggcgccaag ggtaacgaga agagcctgtt ccgttgccgc aaggaagcca tagacatcaa
      241 tcgtgatcgt cgtccggatc tggtgtgcta cttcaagccc gatgtcgcca acttccaacc
      301 aggcgatctg aacggtgtgc tcaagggtaa gaccaagaag ggtgaaatga ttgaaggcac
      361 tgcagcgctg aaaatctttt caagaaagca ccgcaggttc taccatcatc acaagtagaa
      421 attaattgcg tagcttgccc ccgcctcgag cgggggtttt ttttggcagt gtcgctgcga
      481 tattccaaac taaccagcaa tcacaccgca cgctttgcga aatcacgcaa gcggaagccg
      541 aatagccaaa ggctcccaaa ataaatggcg ataccaagaa acaccagccc agtcaggcgc
      601 gtgactcgcc actggagtgt atccgtcaac cacgccgatt ccgcaccggc cgcccacttc
      661 agagctgcgc ccattaagac caatgccaat ccgactttga gcaggaattc gaaccagcct
      721 ttctggggct tgaaaatgtt gtgcttgcgc agttgataaa aaagcatccc ggcgttgatg
      781 caagcgccca atgatatcgc cagtgccagc cctgcatggc gcaacggtcc aataaaagca
      841 agattcatca attgcgttgc aaccagcgta atgatggcta tcttcaccgg ggtgcgaata
      901 ttctgccggg cgtagaaacc cggggccagc accttgacca gaatcagtcc aagcagcccg
      961 acactgtatg ccaccagtgc attgcgcgtc atgagtacat cctggccatt gaatgcgccg
     1021 tgatagaaca acgttgcaat gagcggtacc gcaagcaacg ccagtgcaac cgcgcacggc
     1081 aacgccagaa ggaaagtgag acgaagtccc caatcgagca gctttgaata ctcttcgccc
     1141 gtgtggtcgg caaagcttcg tgtcaggctt ggcaagagaa ttgtcccgag cgcgacgcct
     1201 agcaatccag tcgggaattc catcagacga tccgcgtagt aaagccatga gacgctgccc
     1261 gttacaagaa atgacgcaaa aattgtattg atgagcaggc ttatttgccc aatggatacg
     1321 ccgaacaccg cgggccccat caatgtcacg atcctccaca cacccggatc ccgtaaattg
     1381 aaacggactc gcggcagcat gcctatcctg gcaagaaacg gaagttgaaa agccaattgc
     1441 agaacacccc ccgcaaacac tgcccaggcg agcgccagaa ccggaggatc gaaataaggt
     1501 gcaagccata gcgcaaatac aatgaatgaa acgttcagca gcgtgggcgt gaacgcgggc
     1561 acggaaaacc ggctccaggt gttcaggatc ccaccggcga gtgaagtcag tgatataaaa
     1621 aaaatgtatg gaaaagtaat tcgcaacagc tgcacggtga ttgcaaactt gtcggccgag
     1681 gacgcaaacc cgggggccgt gacataaatt atcaacgggg cagcgactat gccgacagcg
     1741 gacacaacga tcaatgcggc ggcgagcagc gtggcaacat ggtcgataag ggcacgcgtc
     1801 tcgtccacgg ttctggcagc tcggtattcg cccagaatcg gcacgaaagc ttgcgcaaac
     1861 gcgccctcgg caaacagcct cctgaggaga ttgggaagcc ggaatgcaac gaaaaaagca
     1921 tcggtataga gccctgcgcc aaacaatcgg gcaattacgg catcgcgcac gaatcccagg
     1981 atccgcgaca ccagggtcat gctgctgatc gtcgccaggg ctttaagcaa attcatgaca
     2041 gcgtgggagt tgcgaaatgg acgcgattgt aaccgagtga catccaccga ctggctcagg
     2101 gctcgatccg gttgtcccgt cgatagacca tgagttgcgg acgttcaata ctattcgttg
     2161 caaatgcagc ggcgggacag taccatagcg cccttttccg gggacccggt ccaggactta
     2221 actcaatggc aaatatagcc tcagcaaaga aacgcgcccg ccaagccgag aagcgccgtg
     2281 cccacaacgt cggtcagcgc accgaagtgc gctcggcaat caaaaatgta aagaaagcga
     2341 tcgcagccgg cgataaaaaa gcagctcaag cacaattcca gctatcccag agccgtattg
     2401 attccatcgc ggacaaacgg attgtgcaca agaatacagt atcgaggcaa aaaagccgcc
     2461 tggctgccgc cataaaggca atgtcctgat tgattcgcat ctgttcgaat gaaaaggccc
     2521 gcgcaagcgg gccttttgtt ttgtgacttt gtgacgaccg gaccgtcagg cgaccttggg
     2581 aggtttttgt acctgcagtg attgacgaat ctcttggcgt ccctcgcgga tttgtagttc
     2641 attgtcccgg cagaacgtca tcaaaaactg gaaagcttcc gggctcatgt gctcgaggcg
     2701 gcgatcgacc accagacaat ttactcccga tgcgcttacc ggcttggtgt aaggcgaata
     2761 gctattgcgc tgactatcac caaacaacga catgcagccg caaagcctct cggcccagtc
     2821 gccgggctgg aaaatttctc cattgccctt gaggccaacg ataatcagtt caacgattgg
     2881 aggaccggac atatcaatct ctcgcttcgt taacatgcta gcatattttg cagtgcacaa
     2941 aagacgttta tctcactctt aaagagcgca ttctaaagtt ctgattggag gactgtacgc
     3001 gtcgcagaac ccaaccgaat ttcggacaaa aatacggccg ccgggtgcaa catcagcccg
     3061 ctcgaaaata gtggctgggc gctgtaggcg agacccggga tagtgtgacg acctaggcat
     3121 gtgcgtgcct cagttcagcg acttccagag attgcggcac cgtaatactt gcttcggcgg
     3181 gagattcgga taaatatatg aagacgaaac ttcaagcatt tcaatggcaa gcacggcggc
     3241 tttttgccgc cgtgttgttt ttggaggcgg ggaaacggtg cggatgcaaa acatgatgca
     3301 acacgtaatg aaaacctatg cgcggcagag agtggctttc tctcggggca acgggccatg
     3361 gctttgggat ctgcaaggca accgctatct tgactgcgtc agcggggtgg ccgtgaatgg
     3421 acttgggcat gctcacccgc ggctggtcga ggccgtgagc gcccaggcag cgcgtgcgat
     3481 gcatgtttca aacctgtacg agattcccga acaggaggaa cttgccgcac gcttatgcgc
     3541 gcttgccggg atggacagcg ccttcttttg caattcggga tgcgaggcga acgaagccgc
     3601 tatcaagctg gctcgtcttt tcgggcacca acaaggaatc gaatctcctg ccatcgtggt
     3661 catggaaaat gcattccacg ggcgcaccat agctacgctg tcggccaccg gaagtcgtaa
     3721 ggtgcaagcc gggttcgagc cccttttgac ggggtttgta cgggttccct acaacgatgt
     3781 cgcggcgctg atgcaggtcg ccgaagtgaa tccgaacatt gtcgcggtgc tcgtggaacc
     3841 ggtgcagggc gaaagcggca tccagatacc cgaagacaac tacattccgc gactgcgcaa
     3901 gatttgcgat gaacatggct ggttactgat gcttgatgaa gtccagaccg gggtggcaag
     3961 gaccggcaga tggtttgccc atcagcatct ggacgtcaaa ccggacgtta tgtccctggc
     4021 aaaaggactc ggcaacggat tcccaatagg cgcttgtctg gctcgcggtc ctgccgccac
     4081 cgtatttact ccggggaagc acggttctac attcggcggt aacccgctgg cgtgtgtgtg
     4141 cgcgctcacg acgctgtcca ttatcgagga cgaggacctc gtccaacggg ttgccaaaat
     4201 cggccagcaa atcctggatg gcattgcaaa gaaccttgcc ggagctcctg gcgtgaagca
     4261 ggtccgtggc aaaggtctga tgattggcat cgaactggat cgcccctgcg cagatctggt
     4321 gacccaagga ttggaaaacg gccttgtgat taacgtaaca gcagaaaatg tcgttcgcct
     4381 gctgccgcca ttgaacctgc aagattcaga agtgctactg ctcatcgacg gtgtttccag
     4441 gttgatcaga cagttttcgc atgccagcgt cgcgctagcc agctgacttt gacatgcaga
     4501 caccgcggca ttttttgcag ttcagggact tttcgcgcga tgagttcgct catttgttcg
     4561 agcgtacgca atggatcaaa cagcagttca agtcctacaa gacctatcgt ccattgttcg
     4621 atcggacttt agtcatgatt tttgagaagc agagcacccg cacacgattg tcgttcgaag
     4681 ctggcatgca ccagctgggc ggctctgcga tattcctgag taccagagat tcgcagctcg
     4741 gccgcggaga accagttgaa gacgctgccc aagtcatatc gcgtatgtgc gatgtggtca
     4801 tgatgcgaac attcgatcag gaaataatta aacgctttgc cgaccattcc agggttccgg
     4861 tggtgaatgg cctcaccaac gagtaccacc catgccaaat cctcgcagac atccatacct
     4921 atatcgaaca tcgcggtccc attcagggca aaaccgttgc atggatcggt gattcgaaca
     4981 acgtgtgcaa tacgtggctg caggcggcag aggtattgga tttcaatgtc catgtgtcta
     5041 ccccaccggg ctacgaagtt gaaccggaga gagcaggcct ttacggcaca gaccactatg
     5101 aggaatttac cgacccgatg gaggcggtca ggggcgcaca cctggttacc accgacgtct
     5161 ggacaagcat gggatttgag gccgaaaacg aagaacgaaa gcgggacttt gagcagtggc
     5221 aggtggatgc cgagatgatg aagggagcgg ccagcgatgc cttgttcatg cattgccttc
     5281 cggcgcaccg gggcgaagag gtcgctgcgg cagtgattga tgggccgcaa agcgtcgttt
     5341 gggacgaagc cgaaaacagg ctgcacaccc aaaaagcact catggaatat ctggtcctcg
     5401 gaaaagtggc gtccatttag gccattggcc aaacgtatca gcgcggtgat cgaagctgac
     5461 gggaaagaac gcatttgata cagccgctgt cgaaagcgcg taggacaact acatacgagg
     5521 tctacggtgt ggtggcaaac ggtgccgatg tctcaacagt gaatttaatc tcgaaggagc
     5581 caacatgatt cgattgagtc tctccagtat tgcgctggtt ctatccctcg caatcccagg
     5641 cgccctattc ggcgcggacg acgatgtttc ctggatcgct gacaaaaacg gttgcaaagt
     5701 ggccaaccct ttcccgcagc cgggcgagac aattacgtgg aacggccaat gcaaagacgg
     5761 caaggcggac ggcgaggggt tgctgcaatg gtacgtcggc gggaagcttg cggaccggta
     5821 tgagggatcg ctgaaagaag gttgggcaga aggcaagggt accctgacca ggactgaagg
     5881 tggccgctat tccggtgaat ggaagcacag tttgcaggac gggaatgggc gctacgaggc
     5941 gtcagacgga agctggtacg agggtcaatg gaaagacggc cagccccatg gccacgggca
     6001 gtatcgcacg ccagacggcc gcacagtcgt cggagaatgg gtaaacgggg tttatgaagg
     6061 cgacatggat tccgacgaca atcctaacaa gacgtgaatg gaacaaggaa acgtgaagaa
     6121 aaaaaacgaa gaaaagccga tcaaaaaagt agtgctggct tattcgggcg gactggacac
     6181 atcggtcatc ctgaagtggc tgcaggacac ctatcactgc gaagtcgtga cattcaccgc
     6241 cgacatagga cagggcggtg aagtcgaacc ggcccgccgc aaagcgaaaa aatttggtgt
     6301 aagccagatt tatgtcgaag acctgcgcga agaattcgtc cgcgacttcg ttttcccgat
     6361 gttcagggcg aatgcgctgt acgaagggga atatctgctc ggtacttca
//
LOCUS       DATMZQ010000356         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4979173, whole genome shotgun sequence.
ACCESSION   DATMZQ010000356 DATMZQ010000000
VERSION     DATMZQ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4979173"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..388)
                     /locus_tag="VN496_11470"
     CDS             complement(20..388)
                     /locus_tag="VN496_11470"
                     /inference="COORDINATES: protein motif:HMM:NF027655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HXF17618.1"
                     /translation="MVIRSRSRREMRFDQVRQGQRWLPLIALVLLPLAAPALSDVSQP
                     PPEMLAQAEPTAPVKKPPPAETAPNNKGVVQLLNQVEALNGELSRLRGQIEVLTNDIN
                     NAQKRQRDMYVDLDTRLRRI"
     gene            complement(468..1136)
                     /gene="pal"
                     /locus_tag="VN496_11475"
     CDS             complement(468..1136)
                     /gene="pal"
                     /locus_tag="VN496_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXF17619.1"
                     /translation="MRTSLFAMSLLFLLAACASQGQKSADIEQRDLFDEQQAQQKQAA
                     AQEADKQAKVAKEQEELKRQLDEQQRRQPSQPTDQAIVKPLPATGVGGSQLKGDPWAQ
                     LKEPGSPLAKRSVYYDYNKYDIKEEYAPVIESHAKFLMEHSDLKIAVQGNCDDRGSRE
                     YNLALGQRRAESVKRAMVLSGVGDKQIETVSFGAEKPKALGQDEDSWAKNRRADIVYP
                     NEPQ"
     gene            complement(1136..>2196)
                     /gene="tolB"
                     /locus_tag="VN496_11480"
     CDS             complement(1136..>2196)
                     /gene="tolB"
                     /locus_tag="VN496_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXF17620.1"
                     /translation="EPSEINYRYWKSDGTETMVIGTVVEKSDGNAEVRFRLMDIAKQS
                     QLLGFSYTVKVPQLRFTAHKIADLIYEKMTGDPGVFATKICYVTKGDNRFELQVADAD
                     GFNSDFILAHREPIISPQWSPDGSRIAYVSFERRKPIVFVHNLLDGTRTVLANFEGTN
                     SAPSWSPDGKRLAIVLTKDGTSNLYVIGMDGSGLVRLTSNQSIDTEPSFSPDGKYILF
                     TSDRAGGPQIYRLRADGQGEAERMTFEGSYNVTPRYSPDGKSFIFIHRNEGHFNVAMQ
                     DIASRQMQILTGGGFDQSPTFAPNGKMILYASEVKGRGILAAVSSDGRIKQKITAQSG
                     DIREPAWGPLPNDRRKEQ"
BASE COUNT          414 a          629 c          596 g          557 t
ORIGIN      
        1 tcttgcctgt gttgagctgc taaatgcgcc gtaatcgcgt atccagatcg acgtacatgt
       61 cgcgctgacg tttttgtgca ttgtttatat cattcgtcag cacttctatt tgaccacgca
      121 accggctcag ttcgccgttc agcgcctcta cctgattcag tagctgcact acgcctttat
      181 tgttcggtgc ggtttcagct ggcggcggct ttttaacagg cgcggtcggt tctgcctggg
      241 ccaacatttc tggcggtggc tggcttacat ccgacaatgc cggtgccgca agtggcagca
      301 acaccaatgc aatcaggggc agccatcgct gcccctgcct gacttggtcg aaccgcatct
      361 cgcgcctcga ccgagatcgg atcaccatgg cttttcctta gaaacaccgg ataagaaaaa
      421 agcgcgtcgc gaacgcgacg cgccgagact acgcggacga aaagccatta ctgcggctcg
      481 ttcggataga cgatgtccgc acgccggttt ttggcccagc tgtcctcgtc ctggcccaaa
      541 gctttaggtt tctcggcacc aaaactgact gtctctattt gcttgtcccc gacgccggac
      601 agaaccatgg ctcgtttcac gctctcggcg cggcgctgtc cgagggcaag gttatattcg
      661 cgactgccgc ggtcatcaca attgccttgt accgcaattt tgaggtcgct atgctccatc
      721 aggaacttcg cgtgcgattc gattactggc gcgtattctt ccttgatgtc gtatttgttg
      781 taatcgtaat agacgctgcg ctttgcaagc gggctgcccg gctccttgag ttgcgcccag
      841 ggatctccct tgagctgcga accgcctacg ccggtcgctg gcaacggttt cacgattgcc
      901 tggtcggttg gctggctcgg ctggcggcgc tgctgttcat caagctggcg cttgagttct
      961 tcctgctcct tggcgacctt tgcctgcttg tctgcctcct gcgctgctgc ctgcttctgt
     1021 tgcgcttgct gctcatcgaa aagatcccgt tgctcgatgt cggcactttt ttgtccctgg
     1081 ctcgcacacg cggcaagcaa gaaaagcaag gacattgcaa ataaactagt tctcattact
     1141 gctcctttcg gcgatcgttc ggaagaggtc cccacgcagg ttcacgaatg tctccggact
     1201 gcgcggtaat tttttgcttg atccgtccat cgctcgacac cgcggctaat ataccacgcc
     1261 ccttcacctc ggacgcgtac aggatcatct tgccattggg tgcaaacgtc ggcgattgat
     1321 cgaacccgcc gccagtgaga atttgcatct ggcgagacgc gatgtcctgc atggcgacgt
     1381 tgaagtgccc ttcgttgcga tgaatgaaaa tgaatgattt cccatccgga ctgtagcgtg
     1441 gcgttacgtt atagctgcct tcaaaagtca tccgttccgc ttcgccctga ccgtcggcgc
     1501 gcaaacggta aatttgcggg ccgcctgcgc gatcggacgt gaacagaata tattttccgt
     1561 cgggggaaaa actgggctcg gtatcgatgg actgattgct ggtcaggcga accaatccgc
     1621 tgccgtccat cccgatgacg tacaaatttg atgtgccgtc cttggtcagt acgattgcaa
     1681 ggcgcttgcc gtccggggac catgacggcg cggagttcgt gccttcgaag ttggcgagta
     1741 ccgtacgcgt tccgtccagc agattgtgaa cgaacacgat cggcttacgc cgctcgaagg
     1801 aaacatatgc aatccggctt ccgtcaggtg accattgtgg tgaaatgatc ggctccctgt
     1861 gcgcgaggat gaagtcggaa ttgaatccgt ccgcatccgc gacctgcaac tcgaagcggt
     1921 tgtcgccttt ggtcacgtaa cagatcttgg tcgcaaacac tcctggatcc ccggtcatct
     1981 tttcgtagat gagatccgcg atcttgtgcg ccgtgaaacg cagttgcggc actttgacgg
     2041 tgtaggaaaa gccgagcaat tgggactgct tggcgatatc catcaaccgg aaccgcactt
     2101 cggcattgcc gtcgcttttt tcgaccacag tgccgatgac catggtttcg gtgccgtcac
     2161 tcttccaata gcgataattg atttcggatg gttctg
//
LOCUS       DATMZQ010000357         8874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2216729, whole genome shotgun sequence.
ACCESSION   DATMZQ010000357 DATMZQ010000000
VERSION     DATMZQ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216729"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VN496_11485"
     CDS             complement(<1..931)
                     /locus_tag="VN496_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17621.1"
                     /translation="MAQAKFRVGITKDNIGPSGQPLFAVSALTILRDAGIDYAFLPEY
                     EAELTPATAAKYDALAVMLAKVTRHTVSGSDRKLKLIARFGVGYDMVDVAACTENGVI
                     IAITPDGVRRPVATSELTFILMLAQKVLVKDRLTRAGRWNERANHMGPGLSGRVLGSI
                     GVGNIGSELFRLTAPLGMKHIACDPYVTQESVAPLGVTLVDLDSLFRESDFVCVNCPL
                     NGETTKLVGAREFSLMKRTAYFINTARGPIVDIDALYDAMKDGTVLAAGLDVLPQEPA
                     NPQSRLIAAWQSNEDWIRHRLVLTPHSAFYTPES"
     gene            complement(970..1767)
                     /locus_tag="VN496_11490"
     CDS             complement(970..1767)
                     /locus_tag="VN496_11490"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXF17622.1"
                     /translation="MRTNPVKGKLLKGEPVFGAFGWEFLVPGLPQIVKSCGAEFLLID
                     MEHAGTSYETLKDQAALCRGLDLVPMARVPANQYHYISRALDLGVMGIMAPMVGSADE
                     ARHIVSCTRYPPAGRRGAAFGFASDDYVGGSVADKIKVANERTLVMCLIETAEGIDNV
                     EEIAAVPGVDVLWLGHFDLTNFLGIPAQFDHPKYVSAIAKMTTAAQKHGKVLACATGD
                     DQWSRDYWDKGFRLFAMGIDSMLLQGALKQGLGTLEALKGTVTAKRK"
     gene            complement(1785..2807)
                     /locus_tag="VN496_11495"
     CDS             complement(1785..2807)
                     /locus_tag="VN496_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17623.1"
                     /translation="MSTKFKIGISRDAITANGTIFGEEAFNVLKDSAVEWEFLPDKVS
                     EIPPEYAAEYDAICLMSAKVTRNTLSGSNRKLKLIARFGVGYDTVDVAACTDQGVLLT
                     ITPDGVRRPVAASVIGFVLALAHRMFLKDRLTREGRWAEKGNYLGSGLMGRTLGVIGV
                     GNIGREIFKLAQPFGMKLLGCDPNVPQSSVASLGVTMVDKDTLLRQADYVSINTLLDD
                     STRGLIGAREFGLMKPSAYFINTARGPVVVESALIDALKNHRIAGAAIDVFEQEPVSA
                     DNPLLKMDNVIVAPHAICHTDECMSLLGQSAFKAAVDLAHGRKPHLIVDTAALRHPAW
                     TGKFHA"
     gene            complement(2896..3651)
                     /locus_tag="VN496_11500"
     CDS             complement(2896..3651)
                     /locus_tag="VN496_11500"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXF17624.1"
                     /translation="MFDLSGKVALVSGASRGLGWAMAQSLATAGAHVILNARDASAVK
                     DRVRELTAKGLKSEAAAFDVTDHAAGEASIQGAVRKHGRFDILVANAGIQHRKPITEF
                     EAADFQRVIDTNLTAVWALAKEAAKVMIPARRGRIIMTGSVSAILARPTISAYIASKG
                     AVHALTRELAIELAPHNITANAIAPGYFATEMNTALIENKEFNDWVCKRTPAGRWGGL
                     HEIGPVAVFLASDEASFVNGHILVTDGAFSAAM"
     gene            complement(3754..4518)
                     /locus_tag="VN496_11505"
     CDS             complement(3754..4518)
                     /locus_tag="VN496_11505"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXF17625.1"
                     /translation="MEDLKNKVVLITGASTGIGAAAAIAFGKNGAKVAVHYNKSKAEA
                     EKVAAQVEKAGGRAMLVGGDVTQSAVCREIVDKAVAGYGRIDVLINNAGGLVQRAPIE
                     TITDQLFDEVVNLNVRSAMICTSAAAQYMRKQGSGGSIINVTSVAARHGGGPGASLYA
                     GSKGFVSTITHGLAKELVKDKIRVNAVAPGVIATPFHDRYSTPQMLEGFKATIPMNRL
                     GTAEECAGAFLFLASEQLSGYMTGQILEVNGGQYMP"
     gene            complement(4598..5572)
                     /locus_tag="VN496_11510"
     CDS             complement(4598..5572)
                     /locus_tag="VN496_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXF17626.1"
                     /translation="MAKAIRFHKTGGPEVLQLDDVEVGDPADGQARVRNTAIGLNFID
                     TYHRSGLYPMPLPSGIGLEAAGVVEDVGKGVTSVKAGDRVGYAGGPPGAYSQSRLIPA
                     DRLVKVPEGISDNEVAAMMLKGLTVWYLLRRVYRVEKGETVLFHAAAGGVGLIACQWL
                     KALGVTVIGTVGSDDKARIAKAHGCDHTIVYTRENFVERVKEITGSRKVPVVYDSVGK
                     DTFMGSLDCLQPRGLLCIFGNGSGPVSAFDLNLLAAKGSLYVTRPSLMVYTARRQDLE
                     AGAKELFDVVKSGKVKIEINQTYRLQDAAQAHRDLEARKTTGSTVLLP"
     gene            complement(5733..6986)
                     /gene="phaZ"
                     /locus_tag="VN496_11515"
     CDS             complement(5733..6986)
                     /gene="phaZ"
                     /locus_tag="VN496_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HXF17627.1"
                     /translation="MIYQLHEFHKTVMAPAVVWADTTRHFFTNPLSPLAYTPVSRTIS
                     ANSEMFVRLLRRYPKPEWGIDEVISNGERVTVTREIAIDKPFCQLLHFRKSLDVAQPK
                     LLIVAPLSGHYATLLRDTVRTALVDHDVWITDWADAKMVPLAAGPFHLDDYVDYICEF
                     IRALSPQLNVMSVCQPTVPVLGAVSLMASQNDPAVPKAMIMMGGPIDTRRNPTSVNDF
                     AAGRPHSWFSDRVIMRVPGNYPGFMRKVYPGFLQHASFLAMNPDRHMNAHRQFYNHLI
                     RGDGDSADAHRNFYDEYNAVLDMPAEYYLDTIKVVFQEFQLPKGNWSVRGSLVRPETI
                     RKTALFTIEGELDDISGNGQTEAAQTLARNIPAEKRKHLLAKGLGHYGIFSGRKFRET
                     VYPQIRNFIRLMSQREADLKLVRMR"
     gene            complement(7117..8100)
                     /locus_tag="VN496_11520"
     CDS             complement(7117..8100)
                     /locus_tag="VN496_11520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HXF17628.1"
                     /translation="MKTDAPYAGLTPDVILNAIDSAGFRTDGRLAALNSYENRVYQVG
                     LEDAAPVIAKFYRPARWTDAQILEEHAFSIELAQREIPAVAPLVARNETLHTFGGFRF
                     TLYPRRGGRAPELDNADTLEWLGRFIGRIHAVGAIGPFIERPAIDIETFGEEPGRYLI
                     EGGWIPDDLRDAYASVLDQALCGVHDCFERAGTVRCLRLHGDCHPGNVLWTDDGPHFV
                     DFDDARMGPAMQDLWMLLSGDRAAMTRQLSEVLSGYEDFAELDRREIGLTEALRTLRL
                     VHYSAWLARRWQDPAFPAAFPWFHSTKYWQDQILQLREQVAAMQEPALGPL"
     gene            8255..>8874
                     /locus_tag="VN496_11525"
     CDS             8255..>8874
                     /locus_tag="VN496_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXF17629.1"
                     /translation="MEANASDADIKSRISALITEEFMIASDTPGIQLYVRNKRPPQSA
                     TAKDRVLLYVHGATYPASTAFDLALEGMSWMDYIAAQGWDVYLLDLRGYGRSTRPAQM
                     EQPPEANAPIVTTDVAVRDVATAVDAIMKRRNVPTVSLLGWSWGTSIMAAYTANHNES
                     VNKLVLYAPLWLRTTPGVIGGDGPLGAYRTVTKENAKKRWLNGVPE"
BASE COUNT         1815 a         2702 c         2448 g         1909 t
ORIGIN      
        1 tgctctccgg tgtgtagaag gccgagtgcg gtgtcagcac caaccggtgg cggatccagt
       61 cctcattgct ctgccaggcg gcaatcaggc gggattgcgg gttggccggc tcctgcggta
      121 gcacatcaag cccggcggca agcacggtgc cgtccttcat cgcatcatag agcgcatcga
      181 tatcgacgat cggtccgcgc gccgtattga tgaaatacgc ggttctcttc atcaatgaaa
      241 actctcgcgc gccaacgagt ttggtggtct cgccattgag cggacaattg acgcagacaa
      301 agtccgattc gcgaaataga ctatccaggt ccaccagtgt cacgccaagc ggagccactg
      361 attcctgggt cacgtaagga tcgcaggcga tgtgcttcat gcccaaaggc gctgtcagcc
      421 ggaacagttc cgagccgata ttgcccacgc cgatggagcc cagcacccgg ccggaaaggc
      481 cgggacccat atggttcgcc cgctcgttcc agcgacctgc gcgcgtcagg cgatccttga
      541 ccagcacttt ctgcgcgagc atgaggatga acgtcaactc ggacgtcgcg accgggcggc
      601 gcacgccatc aggcgtgatg gcgatgatta cgccgttctc ggtgcatgcc gccacgtcga
      661 ccatgtcgta gcccacgcca aagcgggcga tcagcttcag cttgcgatct gagccggaaa
      721 cagtgtggcg cgtcactttg gccagcatca cggccagcgc atcatacttt gcggcagtcg
      781 ctggggtcag ttccgcttcg tattccggca agaacgcata gtcgatgccc gcatcccgca
      841 agatcgtcag cgccgatact gcaaagagcg gctggccgct gggcccgata ttgtccttcg
      901 tgatgccgac gcgaaacttt gcctgcgcca taggttcgtc tctccactat cgcgagactg
      961 ccggtgcaat tacttccgct tggccgtcac cgttcccttc agcgcttcca gtgttcccag
     1021 tccttgcttc aatgccccct gcagcagcat ggaatcaatg cccatggcga acagccggaa
     1081 acccttgtcc caatagtcac ggctccattg atcgtcgcct gtcgcacacg cgagcacctt
     1141 gccgtgcttc tgggcagcag tggtcatttt cgcaatggcg ctgacatatt tcggatgatc
     1201 gaactgtgcg ggaatgccga ggaaattcgt cagatcaaag tggcctagcc aaagcacgtc
     1261 gacacctggc accgccgcta tctcctcgac gttgtcaatt ccttccgcgg tctcgataag
     1321 gcacatgacc agcgtccgct cattggctac cttgattttg tcggcaacgc tgccgccgac
     1381 atagtcgtcc gacgcaaatc cgaaagccgc cccgcgtcgt ccggccggtg gatagcgtgt
     1441 gcatgaaacg atgtggcgcg cctcgtcggc cgatccaacc attggcgcca tgatgcccat
     1501 gacaccgaga tcgagcgcgc gtgaaatata gtgatactga ttcgccggca ctcttgccat
     1561 cggcaccagg tcgagccccc ggcagagtgc agcctgatcc ttgagcgttt catagctcgt
     1621 accggcatgc tccatgtcga tcagcagaaa ttccgcgccg caggacttca cgatctgcgg
     1681 tagaccgggc accaggaatt cccaaccgaa cgctccgaaa accggctcac ccttcagcag
     1741 ctttcctttt acgggattgg ttctcatttt tcgctccggg gccgtcaagc gtggaatttt
     1801 cccgtccacg ccggatgccg caacgccgcg gtatcgacga tgagatgggg cttgcggcca
     1861 tgcgccaggt caaccgccgc tttgaacgca ctctgaccga gcagactcat gcattcgtcg
     1921 gtatggcaga tggcatgtgg tgccactatc acgttatcca ttttgagcag cggattgtcc
     1981 gcagaaaccg gctcctgctc gaacacatcg atggcggcgc cggcgatgcg atggtttttc
     2041 aacgcatcga tgagtgcaga ttccacaacc actggcccgc gtgcggtgtt gatgaaatac
     2101 gcactgggtt tcatcagtcc gaattcccgg gcgccgatca aaccacgcgt gctgtcgtcg
     2161 agcagggtat tgattgatac gtaatccgcc tgcctgagca acgtatcctt gtccaccatc
     2221 gtaacgccca gcgaagcgac ggaactttgc ggcacgttcg gatcgcaacc cagcagcttc
     2281 atgccgaatg gctgggctaa cttgaatatt tccctgccga tattgccaac tccgatcacc
     2341 cccagcgtgc gccccatcag cccgctaccc agataattgc ctttttccgc ccagcgcccc
     2401 tcgcgcgtaa gcctgtcctt gagaaacatc cgatgcgcga gcgccagcac aaaaccgatc
     2461 acactggctg ccaccggacg tcgcacgcca tccggggtga tggtcaataa aacgccttga
     2521 tcggtgcacg cggcgacatc gaccgtatcg tagccgacac caaagcgcgc gatcagcttg
     2581 agcttgcgat tgctacccga caaagtgttg cgcgtgactt tcgcggacat caggcatatg
     2641 gcatcgtatt cagcagcgta ctcgggggga atctcggaga ccttgtctgg aaggaattcc
     2701 cattccacgg cggaatcctt caggacgttg aaagcctctt cgccaaaaat agtcccgttc
     2761 gcggtgatcg catcgcgcga gattccaatc ttgaacttgg tggacatggc atctccctat
     2821 ctctctttat ggacagcggt cctcgcggcg aggtgaagca cgcatctcgt taaagtgcta
     2881 ttcaaagcac tggttttaca tcgccgcgct gaaggcgcca tctgttacca gaatgtggcc
     2941 gttgacgaat gaggcttcgt ccgatgccag aaagaccgcc accggaccga tttcatgcag
     3001 cccgccccag cgccctgccg gcgtgcgttt gcaaacccag tcgttgaact ccttgttctc
     3061 gatcagggcg gtattcattt ccgttgcaaa ataacccggc gcaattgcgt tcgccgtgat
     3121 gttgtgcggc gcgagttcga tggccagttc gcgggtcagc gcgtgtacgg ctcctttgct
     3181 tgcgatataa gctgaaatag ttggacgggc aagaatcgcg ctgaccgatc cggtcatgat
     3241 gatacggccc cggcgtgccg ggatcatgac tttcgccgct tccttggcaa gcgcccagac
     3301 ggccgtcagg ttcgtgtcaa tgacacgctg aaagtcggcg gcctcgaact ccgtaatggg
     3361 cttgcggtgt tgaatgcccg cattggccac aagaatgtcg aagcgaccgt gttttcggac
     3421 agcgccctga atactggctt cccctgcagc gtggtcggtg acatcgaagg cagccgcttc
     3481 gcttttgagg cctttggcgg tcagttccct gacccggtct ttcaccgcgg atgcgtcgcg
     3541 cgcattcagg atcacatgcg cgccggcggt tgccagcgat tgcgccattg cccaacccag
     3601 gccgcgcgaa gcgccgctga ccagggcaac cttgccgcta agatcaaaca tcgtgcgtcc
     3661 tccaaatcct gttgttattg ttcgttctgt gcgctggtga agatgtttac gtcccatcac
     3721 gcttcatgtc ggccacgctg gcacggcgcg cagtcagggc atgtactgcc cgccattgac
     3781 ctccaggatc tgcccggtca tgtaaccgct gagttgctcg gaagccaaaa acagaaatgc
     3841 gccggcgcat tcttccgcgg ttccgagacg attcatcgga atggtcgctt tgaagccctc
     3901 gagcatttgc ggcgtcgaat agcggtcatg gaacggagtc gcgattactc caggtgcaac
     3961 tgcgttgacc cggatcttgt ccttcaccag ttcctttgcc agtccgtgcg tgatggtgct
     4021 gacaaaaccc ttcgacccgg cgtagagcga tgcgcctggc ccgccgccat ggcgtgcggc
     4081 gaccgaagtc acattgataa tcgatccgcc gctaccttgt ttgcgcatgt actgcgcagc
     4141 ggcgctggtg cagatcattg ccgaccgcac gttcaggttg acaacttcgt cgaacaactg
     4201 gtcggtgatc gtctcaatcg gcgcacgctg caccagaccg cccgcattgt tgatcaacac
     4261 atcgatgcgg ccgtagccag ctactgcctt gtccactatc tccctgcaga cagcggattg
     4321 cgttacgtcg ccgccgacca gcatcgccct gccgccagcc ttttccactt gcgccgcgac
     4381 tttttcggct tccgctttgc tcttgttgta atgcacggcc accttggcac cattctttcc
     4441 aaaggcaatc gcggcggccg cgccgatgcc cgtgctggct ccagtaataa gcacgacctt
     4501 gttcttcaga tcttcgatca ctgctgccct ctccggaacg gttacggcgt tacccgatta
     4561 gtaatgaaat atatttttgt cgttgggcct ttgttcgtca gggcagaagc acggtggaac
     4621 ccgtcgtctt gcgtgcttcc aggtcgcgat gcgcttgcgc agcatcctgc aatcggtagg
     4681 tctggttgat ttcgattttg accttgcccg acttcacgac atcgaacagt tccttcgcgc
     4741 cggcttccag gtcctggcgg cgggccgtat aaaccatcag cgaaggccgc gtcacataga
     4801 gtgaaccttt cgcagccagc agattgaggt cgaaagccga caccggacct gagccgttgc
     4861 cgaaaatgca cagcaggccg cgtggctgca ggcagtccag tgaacccatg aaggtgtctt
     4921 tgccaacgga gtcgtacacc accggcactt tccggctgcc ggtaatttct ttcacgcgct
     4981 cgacaaaatt ttctcttgtg tagacgatgg tatggtcgca tccgtgcgct ttggcgattc
     5041 tggctttgtc atccgagcct accgtgccta ttacggtcac gcccagcgcc ttcagccatt
     5101 gacaggcgat gagtcccaca ccgcccgccg cggcatgaaa caaaacggtt tcgcctttct
     5161 ctacccgata gactcgacga agcaaatacc acaccgtgag ccctttgagc atcattgctg
     5221 caacttcgtt gtccgatatt ccttcgggca ccttcaccag gcggtcggcg ggaatcaggc
     5281 gcgattgcga gtacgctcca ggcggccctc cggcgtaacc cacgcgatcg ccagccttga
     5341 cactcgtcac gcctttgccg acgtcttcca ctacgccggc agcttccagg ccgatgccgc
     5401 tcggaagagg catcggatac aggccagagc ggtgataggt gtcgatgaag ttcaggccga
     5461 tcgcggtatt gcggacccgc gcttgtccgt ccgccggatc gcctacttcg acatcatcga
     5521 gctggaggac ttccggcccg ccagtcttgt ggaaacgtat cgcctttgcc atgtctgctc
     5581 ccggatattt gtatttttaa aatggatgcc gctatcggcc agggccgatt catcttggtg
     5641 ctggcaccgg aataccggtc aatatgtcct gtaactggtt gtctatgcaa tccatgctgc
     5701 gttcccacac cggacaggac ggcagctgca gcttatctca ttcgcaccag cttcagatct
     5761 gcctcacgtt gggacatgag tcgaatgaaa ttgcggatct gcgggtaaac cgtttcgcgg
     5821 aatttgcgcc cgctgaaaat gccgtaatgg ccgagccctt tcgccagcag atgtttgcgc
     5881 ttttccgcag gaatgttacg cgctagcgtt tgagcggctt ccgtctggcc attgcccgaa
     5941 atatcgtcca attcaccttc aatggtaaac agcgcagtct tgcggatggt ctcaggccgc
     6001 accagggagc ctcgcaccga ccaattcccc ttcggcaact ggaattcctg gaatacgacc
     6061 ttgatggtgt ccaggtagta ctccgccggc atatccagaa cagcattgta ttcgtcgtag
     6121 aagttgcgat gtgcgtcagc gctatcgccg tcgccgcgta tcaggtgatt gtagaattgc
     6181 ctgtgcgcat tcatgtgacg gtcaggattc atcgccagaa aactcgcatg ctgcaaaaac
     6241 ccgggataga ccttgcgcat gaatccaggg taattccccg gcacgcgcat gatcacgcgg
     6301 tcggaaaacc aggaatgcgg ccgtcccgcc gcaaaatcgt tcaccgaagt gggattgcgg
     6361 cgtgtatcga tcggtccacc catcatgatc atcgccttgg gcacggccgg atcgttttgc
     6421 gaagccatca aggacaccgc gcccagcacc ggtacagtcg gctggcatac cgacatcaca
     6481 ttcagctgtg gcgaaagcgc tctgatgaac tcgcagatat agtcgacata atcgtcgagg
     6541 tgaaatggac cggctgccaa cggcaccatc tttgcatccg cccagtccgt aatccagaca
     6601 tcgtggtcga ccagcgcagt gcgcactgta tcgcgcaaca gggttgcata gtgaccggac
     6661 agcggcgcga caatcaacaa cttgggctgc gcaacgtcaa gcgacttgcg aaaatgcagc
     6721 agttggcaga acggcttgtc gattgcgatt tcgcgcgtaa cagtcacgcg ttctccattg
     6781 gaaatcactt catcgatgcc ccattcgggt ttcggatagc gccgcaggag ccgcacgaac
     6841 atttcactat tggcggatat tgtgcgcgac accggcgtgt aagccagcgg actcaacgga
     6901 ttggtaaaaa agtggcgggt ggtatcggcc cagacaacgg cgggggccat cactgttttg
     6961 tggaactcat gcagctgata aatcattggg cctcgttttc ggagttcggc aaagcaaaac
     7021 cgatctctca aatatggaac gctgtaaaaa ccggctcatc gactgtaaag actccgtcat
     7081 ggagcgtcaa gaaaccattg atggcctgta catgcgttac aacggcccga gcgcaggttc
     7141 ctgcatcgca gccacctgct ctcgaagctg caggatctgg tcctgccagt atttcgtact
     7201 atggaaccat gggaacgctg cggggaatgc cggatcctgc caccgccgtg ccagccaagc
     7261 cgaataatga accagccgca aggtgcgcaa tgcttccgtt aggccgattt cgcgccgatc
     7321 gagttccgca aagtcctcat agcccgaaag gacctcagag agttgtctcg tcatcgcagc
     7381 ccggtcgccc gatagcagca tccacaggtc ttgcatcgca ggtcccatgc gcgcgtcgtc
     7441 aaaatcaaca aagtgcgggc catcgtcagt ccacagcaca tttcccggat ggcagtcgcc
     7501 gtgcagccgt aggcaacgga cagtcccggc acgttcaaaa cagtcatgca cgccgcacag
     7561 tgcctgatca aggacactgg cataggcatc gcgcaagtcg tctggaatcc agccgccttc
     7621 gatcagatat cggcccggct cttctccgaa agtctcgatg tcgatcgctg gtcgctcgat
     7681 aaagggcccg atcgccccta ccgcatggat ccggccgatg aaccttccca gccactcgag
     7741 ggtatcagcg ttgtccaatt cgggcgcgcg acctccgcgg cgcgggtaca acgtgaaacg
     7801 aaatccccca aaggtatgca gcgtctcatt tcgagcgacc aatggcgcga ctgccggaat
     7861 ctcacgctgt gcaagctcta tggagaaagc atgctcttcg agtatctgtg cgtcggtcca
     7921 gcgcgcagga cggtagaatt tggctattac gggcgccgca tcctcaagcc cgacctgata
     7981 gaccctgttc tcataactgt tcaacgcagc caggcgtcca tcggtgcgga atccggccga
     8041 gtcaatcgca ttcaaaatca catcgggggt caaacccgca tagggcgcgt ccgttttcat
     8101 attgcgattg taacgacatg acgtacgcaa tctctttcgt taaagtgttg ttgccggaac
     8161 ccaccgcatc cactagattc agagaggtgc tcccttgcac accgccatcc gcgtttgcgt
     8221 tattgccatt gcgtttttaa ttggaggcgt ttgcatggag gccaacgcca gtgacgcaga
     8281 catcaagtcc agaatctccg cactgatcac cgaagaattc atgatcgcgt ccgatacgcc
     8341 gggaatccag ctgtacgtgc ggaacaaacg tccgccacag tcagcgacgg cgaaagatcg
     8401 ggtgctgctg tacgtgcacg gcgcgactta tccggcatcg acagcctttg accttgccct
     8461 ggaaggcatg tcgtggatgg attacatcgc cgcacagggc tgggatgtgt atctgctcga
     8521 cctgcgcggc tacggccgtt ccacgcggcc ggctcaaatg gagcagccgc ccgaggctaa
     8581 tgcgccgatc gtgaccaccg acgttgcagt tcgtgatgtg gcgaccgcgg tcgacgctat
     8641 catgaagcgg cgtaatgtac cgacagtcag tttgctcggc tggtcctggg ggacttccat
     8701 catggccgcg tacacagcga accataacga gtcagtcaat aaactcgtgc tgtatgcgcc
     8761 gctttggttg cgtaccacgc cgggcgtgat cggcggcgat ggaccgctcg gcgcctatcg
     8821 caccgtaacc aaggaaaacg ccaaaaagcg ctggctgaac ggtgtgccag agga
//
LOCUS       DATMZQ010000358        20041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4086406, whole genome shotgun sequence.
ACCESSION   DATMZQ010000358 DATMZQ010000000
VERSION     DATMZQ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4086406"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VN496_11530"
     CDS             <1..663
                     /locus_tag="VN496_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HXF17630.1"
                     /translation="KADVSSLVKASVSGEALLPPVRQWIERRGITVHQCYGTADLGLI
                     AYETEPMGGLVVDEGVIVEIVRPGTGDPVAAGDVGEVVVTSLCMEYPLIRFATGDLSA
                     VAPGVSACGRTNMRIKGWMGRADQTTKVKGMFVHPSQVDQVVKRHAEILKARLSVEHD
                     AEGNDCMRLRCEVRDRPDGLSKAIADSVRDVCKLRGEVEFADIGSLPNDGKIIEDLRK
                     YD"
     gene            714..1301
                     /locus_tag="VN496_11535"
     CDS             714..1301
                     /locus_tag="VN496_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348104.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HXF17631.1"
                     /translation="MSNGPISKYPVPKLQELPEDIRARMLEVQQKAGFIPNVFATLAH
                     RPEEFRAFFAYHDALMLKQSGLSKAEKEMIVVATSGLSQCPYCVIAHGAILRIYAKDP
                     QLADLVAIHHRKADLTPRQSAMLDFACKLSRTPEAVTQTDHDMLRNHGFSAEDIWDIG
                     GITAFFALSNRMAHLIDMQPNDEFYLMGRLPPAAK"
     gene            1433..4093
                     /gene="aceE"
                     /locus_tag="VN496_11540"
     CDS             1433..4093
                     /gene="aceE"
                     /locus_tag="VN496_11540"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXF17632.1"
                     /translation="MAALPDDDPQETNEWLDALDGVLQHEGPERAHYILEQLIEKARR
                     SGAYLPYSANTAYINTIPPGQEERSPGDHELEHRIRSYVRWNAAAMVLRANKHSSELG
                     GHIASFASAATLYDVGFNHFWHAPSANHGGDMILVQGHSAPGVYARAFMLGRLSREQM
                     DNFRQEVDGKGLSSYPHPWLMPDFWQFPTVSMGLGPIMAIYQARFMKYLQDRGLADTT
                     GRKVWCFLGDGEVDEPESMGAIGVAAREKLDNLIFVVNCNLQRLDGPVRGNGKIIQEL
                     EADFRGAGWNVIKVIWGSYWDPLLMKDKNGTLVKRMEEAVDGEYQTFKSKDGAYVRKH
                     FFGKYPELLEMVANMSDDDIWRLNRGGHDPHKIYAAYASAVKTEGQPTVILAKTIKGY
                     GMGEAGEAQNITHQQKKMGTTSLRAFRDRFNLPVADDKLEDMPYLTFEEGSAELDYMR
                     KHREALGGYLPARRRKGDTLDIPPLSALEPLLKSSEERESSTTMAFVRILNILVKDKS
                     IGKRVVPIVPDESRTFGMEGMFRQLAIWSSVGQLYTPQDADQLMFYKEDKHGQILQEG
                     INEPGAMSSWIAAATSYSNHGVTMIPFYIYYSMFGFQRVGDLAWAAGDQRARGFMLGG
                     TSGRTTLNGEGLQHEDGHSHILSSTIPNCISYDPTYAYEVAIIIQDGLRRMFKEQEDV
                     FYYITLLNENYVQPAMPKGMEDGVIKGMYRLSEGKGKKDAPRVQLLGSGAILREVVAA
                     QDLLEKDFGIAGDVWSCPSFNELRREGLAVQRWNLLHPEEKPRETYVGRCLNSAPGPV
                     VAATDYIKLFADQIRPFIYKHYVVLGTDGYGRSDYRRQLRKFFEVDRYYVAVAALKAL
                     ADEGSIPASTVTKAIKKYGLDPDKPNPATV"
     gene            4225..5547
                     /gene="aceF"
                     /locus_tag="VN496_11545"
     CDS             4225..5547
                     /gene="aceF"
                     /locus_tag="VN496_11545"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HXF17633.1"
                     /translation="MGSLRDVLVPDIGDFKNIPVIEVLVKPGDSVKAEDSLITLESDK
                     ATMEVPAPFAGVVKELKIKTGDKVSEGAAILSLEVSEAGAAQPSTAPAAPQKPAPAPA
                     PVAPTQMAPPASLAPPTSQLQAAAAPATINEEGFSRAHASPSVRRFARELGVDLGRVK
                     GSGPKERILKEDVAGFVKSALAQTSAGGGGGPSLQLGLPAWPQVDFAKFGPVENLALS
                     RIRKISGPALHRNWVSIPHVTQNDEADITELEAFRKSMADEAQKQGVKLTPLVFIMKA
                     VVAALKNHPEFNASLAPSGDALIVKRYYHIGVAVDTPGGLVVPVVRNVDQKGILQLAK
                     ELAETSTKAREGKLGPADMQGGTFSISSLGGIGGSHFTPIINAPEIAILGVSKSAMKP
                     VWKDGQFVPRLIIPLSLSYDHRAVDGAQGARFITNLTTILSDLRRVLL"
     gene            5611..6282
                     /gene="nadD"
                     /locus_tag="VN496_11550"
     CDS             5611..6282
                     /gene="nadD"
                     /locus_tag="VN496_11550"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXF17634.1"
                     /translation="MNSASPIGVLGGTFDPVHMAHLRVAEEFADAIELAKVRFIPTGT
                     PPHRGIPRVAGAHRLEMVRLAINGNPRFEADDREIRREGVCYTFDTLNELRAELGTRP
                     LCLLMGSDAFSTLTSWHRWEELFDLAHIVIAHRPGLSAQLLQSSLPGPLRKIYLQRLS
                     ASTRILRRDSAGAVYVMQVTALDIAATQIRGLLAREGSARYLVPEAVLDYIDRNSLYK
                     ERDAR"
     gene            6272..6658
                     /gene="rsfS"
                     /locus_tag="VN496_11555"
     CDS             6272..6658
                     /gene="rsfS"
                     /locus_tag="VN496_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXF17635.1"
                     /translation="MHVEAVKQAAVEALEEIKGKDITVLDVRKMTSLFDYMIVATAES
                     ARQTKALARNVQEKLKHLGVRVQGLEGEKTGEWVLVDLGDIIVHVMQPAVRAYYNLEQ
                     LWGGRPPSPHSTTWRNVPGAEPPAAL"
     gene            6674..7156
                     /gene="rlmH"
                     /locus_tag="VN496_11560"
     CDS             6674..7156
                     /gene="rlmH"
                     /locus_tag="VN496_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXF17636.1"
                     /translation="MKLHILAVGNKPPDWVAAGFAEYARRMPRGARIELTEIKPAPRG
                     QQAASPALVARILKAEKSRLVAAIPADCVRIALDEHGKPLRTADLARKLAAWIEGGRD
                     VAFAIGSADGLDPDFKANADLLLSVSAMTLPHAMVRVLLAEQLYRAMSMIQNHPYHRE
                     "
     gene            7176..7823
                     /locus_tag="VN496_11565"
     CDS             7176..7823
                     /locus_tag="VN496_11565"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXF17637.1"
                     /translation="MNELTNRTPVPALEKHIYLASRSARRRDLLKQMGVNFEMLLLRE
                     GAGRDADFDEAQIANESPREYVMRLARLKAEAGWLRLEQRRLMRHPVLAGDTTVAFNN
                     IILAKPKDREDAVAMLKQLSGTTHEVYTAVAVKFHDALQEALSVTEVRMRVLTDDEIR
                     RYVASGDPMDKAGAYGIQGKASMFIESINGSYSGVVGLPMYETSQLLTHFGFQLL"
     gene            7820..9274
                     /gene="rng"
                     /locus_tag="VN496_11570"
     CDS             7820..9274
                     /gene="rng"
                     /locus_tag="VN496_11570"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HXF17638.1"
                     /translation="MNEEILINVTPQETRVAVMQQGVAQELHVERSASRGIVGNIYVG
                     EVSRVLPGMQSAFIQIGLDRAAFLHVADIWSHRQNQEETRPIERILQDGQRLLVQVIK
                     DPIGTKGARLSTQISIAGRLLVYLPQESHIGISQRIEDENERALLREKLQHLVPPEER
                     GGYIVRTMAETASEKELAADVGYLRKLWSDIEEKSRAIAAPALLYQDLSLSQRVLRDF
                     VNDESGRILVDSRETYLRMQAFATEYTLSFVDRIHHYTGDRPLFDLYGVEEEIERALA
                     RRVPLKSGGYLIIDQTEALTTIDVNTGGFVGGRTFDDTIFKTNLEAAQVIARQLRLRN
                     LGGIIIIDFIDMDNEVHRNAVISEFKKALLPDRTRMTVNGFTQLGLVEMTRKRTRESL
                     AHILCEPCPTCQARGEIKTAQTVCYEILRELVRESRQFNAREFRILASQKVIDIFLDE
                     ESQSLAMLEEFIAKPIALQVESIYTQEQYDIVLM"
     gene            complement(9325..10749)
                     /locus_tag="VN496_11575"
     CDS             complement(9325..10749)
                     /locus_tag="VN496_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17639.1"
                     /translation="MVQERLSSNIPARLDRLPWSAWHWRVVIALGIAWMLDGLEVTLV
                     GSIGSVLERPDTLGLTAEQIGWAGSGYVGGAVIGALFFGRLVDSAGRKRLFILTLAVY
                     VAATLASAFSYNFTTFLLCRLFTGFGIGGEYAAINSAIDELIPARVRGRVSLAINGSF
                     WLGAAVGAGLSLVLLDTRLFHADVGWRIGFGLGALLAIAILLVRRDVPESPRWLLIRG
                     RRDDAERAVAGIELAVARQHGSLQPPDRTLSIVVRPQPSLSEFAHLLLRRYPNRTLLG
                     LSLMVSQAFFYNAIFFTYALVLTRFQGVSASQVGRYIFPFAIANFLGPVLLGPLFDRF
                     GRRRMIALTYGLSGVALAATGFGFMQGWLNAVTQTVCWSGVFFLASAAASSAYLTVSE
                     VFPLEMRALSISAFYAVGTGIGGFIAPAFFGMLIATGSRDSVFIGYVVGALLVVAAAA
                     VALRHGVDAERKSLEEVAAPISAA"
     gene            complement(10876..12042)
                     /locus_tag="VN496_11580"
     CDS             complement(10876..12042)
                     /locus_tag="VN496_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HXF17640.1"
                     /translation="MPLLRDISLSAVVAGFVTVLVGFTSSGVLVFQAAHAVGAGSTEI
                     ASWMWALGLGMGVTCMALSLRYRMPVVTAWSTPGAAMLITSAAGISLAQATGAFLVSA
                     LMITLAGFSGWFERAMNRIPVSIASGMLAGVLLRFGLELFVVMKAQFVMAFAMFAAYL
                     IMRRKHPRYAVIAAFALGIAIAVAQGTLHLESVTFELATPVWVTPAFSLSALVGVAVP
                     LFVVTMASQNVPGVAVIRASGYPIPISPVVGWTGLANVLLAPFGAFAINLAAITAAIC
                     MGSEAQEDPKRRYVASMAAGFFYLLIGLFGASVGALFAAFPHELVAAIAGLALIGTIG
                     NGLSAAMAAEQEREAALITFLVTASGVTLLGIGSAFWGLLAGAVTLGVLRTRRA"
     gene            complement(12155..14095)
                     /locus_tag="VN496_11585"
     CDS             complement(12155..14095)
                     /locus_tag="VN496_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HXF17641.1"
                     /translation="MTSDLENQVTAYQQWRDELRDCIQSYQNWLEDHGHADIQRSLRI
                     YDLVESLRNDRMILAFLAEFSRGKTELINAMFFSDFKQRLLPSDVGRTTMCPTEIFFD
                     SAEEPYIKLLPIESRKGDESIAALKHKPVEWVKIKLNLASQEEMATAMSSLAQVKSVT
                     VEEASLLGLLDETDFVTTTVVVKQGGKVEIPCWRHALVNYPHPLLKKGLVILDTPGLN
                     ALGTEPELTLSMIPSAHAVLFLLAMDTGVTKSDLDVWQKYVQNYVTRRIAVLNKIDLM
                     WDDLKSDDQIEIGIERQLIETSRILGLPRDHVIALSAQKGLIGRIRGDAALLARSRIE
                     VLERLLAEEVIPSKQQILRAAVSREIGNMLETSLQSATAQLISTRGELQEMSRLSGKN
                     RDLAKLLLAKLEEDKGHYMRHMESYKRSSTTVFKQGNVLLGTLAEERLDEMMSRSLAS
                     IEGSWTTAGLMRSMQALFDTFSRQAEKILEFATETRNYVDGVYGEFHKLYGFPKMTPP
                     ALNLERHILRMHALHQNTERFCKDPVNVMTEKHFLVRRFYSGLVSHARELFTQLRLEL
                     DAWVNSALTPLSVQLKEHQKLLERRVENLRKVAGDINTLQERVRQLEQQKLQFGKQVE
                     ELMQIRDVLQRDPAQIKVAKVA"
     gene            complement(14092..14400)
                     /locus_tag="VN496_11590"
     CDS             complement(14092..14400)
                     /locus_tag="VN496_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HXF17642.1"
                     /translation="MPDTWYRYHPDRGLLTLNLHVQPNARRTEFAGLHGGHLKVRLNA
                     PAVEGKANECLLRFLADKFDLPGGRVMIRRGSHGRTKIVEIYGQGATLMARVKQLLHS
                     "
     gene            complement(14415..14987)
                     /locus_tag="VN496_11595"
     CDS             complement(14415..14987)
                     /locus_tag="VN496_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXF17643.1"
                     /translation="MNQVLIFLLETFLGLFSLALLMRFYLQTVRAPARNPLSQFLAAL
                     TDFIVRPTRRVVPGLWGYDLSTLLLAWVTELVLVSSVLALKGYQVGPGVGSAVVGLML
                     LSTLNLLKLFIYILMVAVFAQAILSFVAPRGPATSVLASLTRPFLSVFQRRVAPVGGV
                     DLSPLFVLVICQILLMWPVSALEQAITRML"
     gene            complement(15018..15797)
                     /gene="proC"
                     /locus_tag="VN496_11600"
     CDS             complement(15018..15797)
                     /gene="proC"
                     /locus_tag="VN496_11600"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXF17644.1"
                     /translation="MASALIGGMLQRGFKPGELRAIDVMQPARQRLEQRFGVHASAVL
                     SSEIAEDVVLLAVKPQQMREMATELRAYLRGQLVISIAAGIRTYDLARWLGGHDRIVR
                     AMPNMPALIGSGVSGLFAAANVGQADRDCAQDILGAVGGVVWVADEPAIDAVTAVSGS
                     GPAYVFYFIEALEQAALELGLNETQAHELAVQTFAGAARLADGSDDPAAVLRARVTSK
                     AGTTEAALRSMETNQVKSKIVEAVHAAARRSSQLGDDFGKP"
     gene            15681..15923
                     /locus_tag="VN496_11605"
     CDS             15681..15923
                     /locus_tag="VN496_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17645.1"
                     /translation="MHAKSLFQALARWLHYINGAKLAGLETPLQHPAYERGGHVAAAY
                     EKYAHRFKAQLWPTGRAARKTLRTAIALFTSFPPSS"
     gene            complement(15898..16437)
                     /locus_tag="VN496_11610"
     CDS             complement(15898..16437)
                     /locus_tag="VN496_11610"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF17646.1"
                     /translation="MNRRKMVIAAALLLALPGMAWSAKPGYLHVTESVDINAPVAKVW
                     GLVKDWNGLHKWHPAFSNTEIRSGANNKVGAVRTLTMADGGAKFDEELDGFSDKKKKY
                     SYRIITDSPLPVADYSSTIQVKSGKTKDTSVLIWKGKFKRKVADNPPAGQDDAGVKKA
                     ISGAYQAGLQNVKKMAETK"
     gene            complement(16567..17472)
                     /locus_tag="VN496_11615"
     CDS             complement(16567..17472)
                     /locus_tag="VN496_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXF17647.1"
                     /translation="MTTIAARLQAVRSRIRVAALGAGRSPDEIGLIAVSKTFSPAAVA
                     EACAAGQFAFGENYLQEALGKIQALSQTRPIGVAPGQEERGPQEGDMRSAGVASPRPL
                     LLPLQWHDAPGQKERGPQEGDMRSSAGVASPQAGPLLLPLEWHFIGPIQSNKTRQIAE
                     HFTWVHAVDRIKIAQRLAAARPATQGPLQICLQVNVSGEGSKSGVRPEEAVALAQALS
                     QLPGLQLRGLMAIPEPTDDIVVQRARFRQVRQLKDEINRQGISLDTLSMGMSDDLEAA
                     IAEGATLVRVGRAIFGERVPFTPDC"
     gene            17583..18623
                     /locus_tag="VN496_11620"
     CDS             17583..18623
                     /locus_tag="VN496_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXF17648.1"
                     /translation="MDIAELLAFVVKNKASDLHLSAGLPPMIRVHGDIRRINLPPMEH
                     KDVHAMVYDIMNDGQRKFYEENLECDFSFAIPNLARFRVNAFVQNRGAGAVMRTIPSK
                     VLTLEELKAPVIFKEIAQYPRGVVLVTGPTGSGKSTTLAAMVNDINENEMGHILTVED
                     PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDIILVGELRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTVDRIIDVFPAAEKEMVRAMLSESLRAVISQTLCKTKDGTG
                     RAAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGQGLGMQTLDQNLTELIKRNIITKEE
                     ARGKAANKDTFN"
     gene            18663..19799
                     /locus_tag="VN496_11625"
     CDS             18663..19799
                     /locus_tag="VN496_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXF17649.1"
                     /translation="MERDQAVKFIHELLRAMVAKRASDLFITAGFPPAFKLDGKMTPV
                     SNQSLTPQHTQELARSLMNDKQAQEFEASKECNFAINPPGIGRFRVNAFVQQARVGIV
                     LRTITTAIPKMEDLGLPPVLKDVAMTKRGLVILVGGTGSGKSTTLAAMIGYRNENSHG
                     HIITIEDPIEYVHDHKNCLVTQREVGVDTDSWESALKNTLRQAPDVILIGEVRERETM
                     EHAIAFAETGHLCMCTLHANSANQALDRIINFFPEERRPQLLMDLSLNLKGLVSQRLI
                     PLKETKGRAAAIEILLNSPLISDLIFKGEVHEIKEIMKKSREMGMQTFDQALFDLYEG
                     GKIGYEDAMRNADSMNELRLQIKLHGKEMRDRDLTTGVDHLNIV"
     gene            complement(19852..>20041)
                     /locus_tag="VN496_11630"
     CDS             complement(19852..>20041)
                     /locus_tag="VN496_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HXF17650.1"
                     /translation="KQKFAGWRAYLFTADLRLPKLIGLSPSRRTPLYNGALECRLYEF
                     KMVSGAMRRQKKGPELDA"
BASE COUNT         4455 a         5929 c         5765 g         3892 t
ORIGIN      
        1 aaagccgatg tcagcagcct tgttaaggca agcgtgtcgg gtgaggcttt gttgccgccg
       61 gtgcggcaat ggatcgagcg tcgcggcatt acggttcacc aatgctacgg gactgctgac
      121 cttgggctca ttgcgtatga gaccgaaccc atgggcgggc tggtggtcga cgaaggcgtc
      181 attgtagaaa tcgtgcggcc gggaaccggc gacccggtgg ctgcaggaga cgtgggcgaa
      241 gtagtggtga caagtttgtg catggagtat ccgctgatcc gctttgctac cggagatttg
      301 tcggctgtcg cgccaggcgt tagtgcgtgc ggacgcacca atatgcgcat caagggatgg
      361 atggggcgcg ccgaccagac taccaaagta aaagggatgt ttgtccaccc gtcccaagtt
      421 gaccaagtcg tcaaacgcca tgccgagata ctcaaagcgc ggctgtcggt tgaacatgat
      481 gccgagggca acgattgcat gcggttgcgc tgcgaagtgc gtgaccggcc cgacggactg
      541 tcgaaggcaa tcgccgacag cgtgcgtgat gtctgcaagc tgcgcgggga ggtggaattt
      601 gcggacatcg gctcattacc caacgacggc aagataatcg aagacctgcg taaatacgat
      661 tgaacctggt gccgcgcgcc gcggttagcc actcaaaatt tcacggagta gcaatgtcga
      721 acggcccaat cagcaaatat ccggttccga aactgcaaga gttgcctgaa gacattcgcg
      781 ccaggatgct ggaggtgcag cagaaggctg gcttcattcc aaacgtgttc gcgaccctgg
      841 cccaccggcc ggaggagttt cgcgccttct tcgcatatca cgacgcgctg atgctgaagc
      901 aatccgggtt gtccaaggcc gagaaagaaa tgatcgtcgt tgccaccagc gggcttagcc
      961 agtgtccgta ctgcgtgatc gctcacggcg caattctgcg catttatgca aaggacccac
     1021 aactcgcgga tttggtggca atccatcatc gcaaggctga cctgacgccg cgccagagcg
     1081 ccatgctgga ttttgcgtgc aagctttcgc gcacgcccga agccgtgacc caaaccgacc
     1141 acgatatgtt gcgcaaccac ggattcagtg cagaagatat ctgggacatc ggaggcatca
     1201 cggctttttt cgccctgtcg aaccgaatgg ctcacttgat cgacatgcaa ccgaacgacg
     1261 aattttattt gatgggtcgg ctccctcctg cggccaaatg acgcagcgca acgccctacg
     1321 ctcagaaaag ccgctctgtt ctgcggtgct ataatttttt aaacaacggg aaccgcgcca
     1381 caaacgcttc agtccctcga atcatcagtt gtaaccttat caaggtagag ccatggcagc
     1441 acttccggat gacgatccgc aagaaacaaa cgagtggctg gacgcgctcg atggagtttt
     1501 gcagcatgaa ggtcccgagc gggcccacta cattctcgag caactcatag aaaaagcgcg
     1561 gcgttccggc gcgtatcttc cgtatagcgc caataccgcg tacatcaaca cgattccgcc
     1621 ggggcaggaa gagcgttccc cgggcgacca tgaactcgaa caccgcattc gatcgtacgt
     1681 ccgctggaac gccgccgcga tggtgctcag ggcgaacaag cactcatcgg aactcggtgg
     1741 ccatatcgcg agctttgcgt cggccgcaac gctgtatgac gtaggattca atcatttctg
     1801 gcacgcgccg tccgcaaatc atggtggcga catgattctc gtgcagggcc actcggcgcc
     1861 gggcgtctac gcacgcgcgt tcatgctcgg gcggctgtct cgcgagcaaa tggacaactt
     1921 tcgccaggaa gtagatggca aggggctgtc ttcctatcca cacccatggc tgatgcccga
     1981 tttctggcaa ttcccgacgg tgtcgatggg attgggtccg ataatggcga tctatcaggc
     2041 gcgcttcatg aagtatctgc aggatcgcgg cctcgccgac accacgggcc gcaaagtgtg
     2101 gtgcttcctc ggcgacggcg aagttgacga gccggaatcc atgggcgcga ttggcgtggc
     2161 cgcgcgcgag aaactggaca acctgatttt cgtcgtgaac tgtaacctgc agcgcctgga
     2221 tggtccggtg cgcggcaatg gcaaaatcat ccaggaactc gaagcggatt tccgcggcgc
     2281 tggttggaat gtgatcaagg ttatctgggg ctcgtattgg gatccgctgc tgatgaagga
     2341 caaaaacggc acgctggtca agcgcatgga ggaagccgtc gacggcgagt accagacgtt
     2401 taaatccaag gatggcgcct acgtgcgcaa gcatttcttc ggcaagtatc cggagctgct
     2461 ggaaatggtc gccaatatgt cggatgacga tatctggcgc ctgaatcgcg gcgggcatga
     2521 cccgcacaag atctatgctg cctacgcatc tgcggtgaag accgaaggcc agcccacggt
     2581 aatcctcgcc aagaccatca agggctatgg aatgggcgag gctggcgaag cgcaaaacat
     2641 cacgcaccag cagaaaaaaa tgggtacgac ttcgctgcgc gcgtttcgcg atcgcttcaa
     2701 cctgccggtg gccgacgaca aactcgaaga catgccttat ctcacgttcg aggaaggctc
     2761 ggccgaactg gactacatgc gcaagcaccg cgaagcgctg ggcggctatc tgccagcgcg
     2821 cagacgcaaa ggcgacacgc tcgacatccc gccgttgtcg gccttggaac cgctgctgaa
     2881 atcctcggaa gaacgtgaat cctcgaccac catggcgttc gtgcgcattc tgaatattct
     2941 ggtcaaggat aaaagcatcg gcaagcgtgt ggtccctatc gtgccggatg agtcgcgcac
     3001 cttcggcatg gaaggcatgt ttcgccaatt ggcaatctgg tcttcggtcg gccagctgta
     3061 tacgccgcag gacgccgacc aattgatgtt ctacaaggag gacaaacacg gtcagatttt
     3121 gcaggaaggc atcaacgagc cgggcgccat gtcatcgtgg atcgctgccg cgacgtctta
     3181 ctcaaaccac ggcgtgacca tgattccgtt ctacatctat tattcgatgt tcggattcca
     3241 gcgcgtgggt gacttggcct gggcggccgg cgatcagcgc gcccggggct ttatgctggg
     3301 cgggacatcc gggcggacta cgctgaacgg cgaaggcctg cagcacgaag acggccacag
     3361 ccacatcctg tcatcgacga ttccgaattg catatcgtac gacccgacct acgcttatga
     3421 agtagccatc atcattcagg acggcctgcg ccgcatgttc aaggagcagg aggacgtttt
     3481 ttattacatc acgctgctga acgaaaatta tgtccagccc gctatgccca aagggatgga
     3541 agatggcgtt atcaagggca tgtaccggct ttcggaaggc aagggcaaga aagatgctcc
     3601 gcgcgtgcaa ctgctcggca gtggtgccat cctgcgcgaa gttgtggccg cgcaggatct
     3661 gctggagaag gactttggca tcgcaggcga tgtctggagc tgccccagct tcaacgaatt
     3721 gcggcgcgag gggcttgcag tgcagcgctg gaacctgctg caccccgaag aaaagccgcg
     3781 cgaaacttat gtcggccgtt gcctgaactc cgcgcccggc cccgtcgtcg cagcaaccga
     3841 ctatatcaaa ttgtttgccg atcagatccg accattcatc tacaagcatt acgtggtact
     3901 cggcacggat ggctatggac gatcagatta tcgccggcag ctgcgcaaat ttttcgaggt
     3961 ggaccgctac tatgttgccg ttgcagcctt gaaagcgctg gccgacgaag gaagcattcc
     4021 tgcttccacg gtcaccaagg caataaagaa atacggtctc gatcccgaca agccaaatcc
     4081 cgcgacggta tgaaacgaca aaatgcagaa cgggcatgtg gagacttgga gcatgcgcga
     4141 tcgtgcagtt atcctcccat ttcgcttttg cgtttactca cccgtcatta ttcatccagt
     4201 ccaacgagca gaataaatta aaacatgggc agcctcagag acgtcctcgt ccccgacatc
     4261 ggtgacttca aaaacatccc ggtcattgaa gtcctggtca aaccaggaga cagcgtcaag
     4321 gcagaggact cgctgatcac gctcgagtcc gacaaggcga ccatggaagt gccggccccg
     4381 ttcgccggtg tggtcaagga attgaaaatc aaaacgggcg ataaggtctc ggaaggggca
     4441 gccattttaa gtttggaagt cagcgaagcc ggcgctgcgc agccgagtac ggcccctgcc
     4501 gcgccgcaga agcctgcgcc cgctcctgcg ccggttgcgc ccacccagat ggctcctccg
     4561 gcatccctcg ctccgcccac atcccaactg caagcggctg ccgcacccgc gaccatcaac
     4621 gaagaaggct tttcgcgtgc acatgccagc ccgtcggtgc ggcgctttgc tcgagagctg
     4681 ggcgttgacc ttggccgcgt caaaggaagc gggccgaagg aacgcattct caaggaggat
     4741 gtcgccggct tcgtaaagtc tgcactggcg caaacgtcag ctggaggcgg tggcggcccg
     4801 agtttgcagc tgggacttcc ggcctggccc caggtcgact tcgcaaaatt cggtcccgtg
     4861 gaaaatctgg cgctgtcgcg catccggaaa atctccggtc ccgccctgca tcgcaactgg
     4921 gtttccattc cgcatgtcac gcagaatgat gaagcggata tcacagagct ggaggcgttt
     4981 cgcaagagca tggcggatga agcgcagaag cagggggtca agttaacccc gctcgtgttc
     5041 atcatgaaag ccgttgtcgc cgcgctgaag aaccacccgg aattcaacgc ctcgctggcc
     5101 ccatcgggcg atgcgttgat tgtgaagcgc tattaccata tcggcgtggc agtagatacg
     5161 ccgggcggtt tggtggttcc cgttgtacgc aacgtcgatc agaagggcat tctgcaattg
     5221 gcgaaggagc tcgccgaaac cagcaccaag gcccgcgaag ggaagcttgg cccggctgac
     5281 atgcagggtg gcacgttttc catatcgagc ctgggcggca tcggcggaag ccacttcacg
     5341 cctattatca atgcacccga aatcgccatt ctcggggtgt cgaaatcggc catgaagcct
     5401 gtctggaagg atgggcaatt cgtgccgcgg ctgatcattc cattatcact ctcctatgat
     5461 catcgggcgg tggacggcgc ccagggtgcg cgcttcatta ccaatctgac caccatcctg
     5521 tcggacctcc ggcgcgtgct gttgtgatgc atgcggccgc acagcgggcg caacgccctt
     5581 gaccgattat cggtcaccgt tcagcgacga ttgaattccg cctcccccat cggcgtattg
     5641 ggcgggactt tcgatcccgt ccacatggcc catctacgcg tggccgagga gttcgccgac
     5701 gctattgagc tggccaaagt gcgattcatt ccgaccggca cgccacccca ccgtggcatc
     5761 ccgcgtgtgg ccggggcgca ccgcctggaa atggtgcgcc tggcgatcaa tggcaatccg
     5821 cgtttcgaag cagacgatcg ggaaatccgc cgggaaggcg tctgctatac attcgatacg
     5881 ctcaatgagc taagggctga acttggaaca cgtccactct gtctcttgat gggatcagat
     5941 gcgttttcta cgctgacaag ctggcatcgc tgggaggaat tattcgacct tgcccatata
     6001 gtaatcgcgc atcggccagg gttgtcggcg cagctgctgc agtcgtctct gcccgggccg
     6061 cttcgcaaga tttatttgca gcggttgtcc gcgtcgaccc gcatcctgcg gcgggacagc
     6121 gcgggagccg tttacgtcat gcaagtcacg gcgctggata tcgcggcaac ccaaatccgc
     6181 ggactgctcg ccagggaagg cagtgcgcgc tatctggtcc cggaagccgt tctcgattac
     6241 attgaccgca atagtctata caaggaacgc gatgcacgtt gaagccgtga aacaagccgc
     6301 cgtcgaggcg ctggaggaaa tcaaggggaa ggacattacc gtcctggatg tgaggaaaat
     6361 gacctccctg ttcgattaca tgatcgtagc gacagcagaa tcagcgcgac agacaaaagc
     6421 gctagcaagg aacgttcagg agaaactcaa gcatctgggc gtacgcgtgc agggactgga
     6481 aggggaaaag accggtgaat gggtgctggt ggacctgggc gacataatcg tgcatgttat
     6541 gcagccggcg gtacgggctt actacaacct ggagcaatta tggggaggca ggccgccatc
     6601 gccgcattcc accacctgga gaaatgtccc tggcgctgaa ccgcctgcgg cgctgtagtc
     6661 ctgaagcagc caggtgaagc tgcatattct ggcagttggc aacaaaccgc cggattgggt
     6721 cgcggccggt ttcgccgaat atgcccggcg catgccccgc ggagcgcgca ttgaacttac
     6781 cgaaatcaaa cccgccccgc gcggccagca ggcggcgtcc cccgctctgg ttgcccgtat
     6841 cctgaaggcg gaaaaatccc gtctggttgc tgccattccg gccgactgtg taagaatagc
     6901 gctggacgaa cacggcaaac cgttgcggac cgccgacttg gcccgcaaac tggctgcgtg
     6961 gatagaggga ggaagggacg tcgctttcgc gattggcagc gcggacgggt tggacccgga
     7021 cttcaaagct aatgccgatt tgctgttgag tgtatcggca atgacgctgc cgcatgccat
     7081 ggtgcgggtg ttgctagccg agcagcttta tcgcgccatg tcgatgattc agaatcaccc
     7141 ttatcaccgg gaatagcgaa taacgccaat ccggcgtgaa cgaacttaca aaccgaacac
     7201 ccgtgcccgc gctggaaaaa cacatatacc ttgcttcgcg cagtgcgcgg cgccgcgacc
     7261 tgctcaagca aatgggcgtg aacttcgaaa tgctgcttct gcgcgagggt gcgggccggg
     7321 atgcggattt cgatgaagcg cagatcgcca acgaatcccc gcgcgaatat gtcatgcggc
     7381 tcgcccggct taaagcagaa gcgggatggc tgcgtctgga acagcggcga ctgatgcgcc
     7441 atccggtgct ggcaggcgat acaacggtag ctttcaacaa catcatcttg gccaagccca
     7501 aggaccgcga agatgcggtg gccatgctga agcagctctc cgggacgacg cacgaggtct
     7561 ataccgccgt ggcagtcaaa ttccacgatg cgctgcagga ggcgctctca gtgaccgaag
     7621 tgcgcatgcg agtgctgacc gacgacgaaa tcagacgcta tgttgccagc ggcgacccga
     7681 tggacaaggc cggtgcatac ggcatccagg gcaaggcttc gatgttcatc gaatccatca
     7741 atggcagcta cagcggcgtg gttggattgc cgatgtacga aacttcgcaa ctgctgacac
     7801 atttcggctt tcagttgctg tgaacgaaga aatactcatc aatgtcaccc cgcaggaaac
     7861 ccgggtcgcg gtgatgcagc agggtgtcgc ccaggaattg cacgtcgagc gcagcgccag
     7921 ccgcggcatc gtcggcaata tctatgttgg ggaagtcagc cgcgtattac ccggtatgca
     7981 atcggcattc atccagatcg gcctggatcg tgccgcgttc ctgcacgtcg ccgacatctg
     8041 gtcgcaccgc cagaaccagg aagagaccag gccgatcgag cgcattttgc aagacgggca
     8101 acggctgctg gtgcaggtta tcaaggatcc gattggcacc aaaggcgctc gactgtcgac
     8161 gcagatcagt atcgccggcc gcctgctggt ttatttgccg caggaatcgc acatcggcat
     8221 atcgcagcgc atcgaagacg aaaacgagcg agcgctgctg cgcgaaaaac tgcagcacct
     8281 ggttccaccg gaagagcgcg ggggctatat cgtgcgcacc atggccgaaa ccgcaagtga
     8341 gaaggaactt gccgcggacg tcggttattt gcgcaagcta tggtccgata tcgaggagaa
     8401 gtcgcgcgcc attgccgcgc cggctctgct ctatcaggac ctgagccttt cgcagcgggt
     8461 actgcgcgat ttcgtgaacg acgaaagcgg gcgcattctg gtggattcgc gtgaaacgta
     8521 cttgcgcatg caggcatttg caaccgagta cacactgtct tttgtcgatc gcattcatca
     8581 ctacaccggc gacaggccgt tgttcgatct ctacggcgtc gaagaggaaa tagaacgcgc
     8641 gcttgcgcgc cgcgtgccgc tcaagtccgg cggttatttg attatcgacc agaccgaggc
     8701 cctgacgacc atcgatgtca acacgggcgg tttcgtcggc ggacggacct tcgatgacac
     8761 catcttcaag accaacctgg aggccgcgca agtgatcgcg cgccagctgc ggttgcgcaa
     8821 cctgggcggc atcatcatca tcgatttcat cgacatggat aacgaagtac accgcaatgc
     8881 ggtcatttca gaattcaaga aagctttgct gcccgatcgc acgcgcatga cagtcaacgg
     8941 ctttacgcag ctcggactgg ttgaaatgac cagaaaacgc acgcgcgaga gtctggcgca
     9001 tatcttgtgc gaaccgtgcc caacctgcca ggcgcgcggg gagatcaaga cagcgcaaac
     9061 cgtctgctat gagatcctgc gcgagctggt gcgcgaatcg cgccagttca acgcgcgcga
     9121 attccgtatt ctggcttcac agaaggtgat cgacatattt cttgacgagg aatcgcagag
     9181 cctggcgatg ctggaagaat ttatcgccaa gccgattgcc ctgcaggtgg aaagcatcta
     9241 cacccaggaa cagtacgata tcgtgctgat gtagtggtgt tcggcgcagc cgcagaggcc
     9301 acgttgccgt ctgtttccgg gcgcttaagc tgcactaatc ggcgcagcga cttcctctag
     9361 cgatttacgt tccgcatcga cgccatggcg cagtgcgact gccgcagcag caaccacgag
     9421 taatgcacca acgacgtagc cgatgaacac gctgtcgcgg ctaccggtcg caatcagcat
     9481 gccaaagaaa gccggcgcaa taaagccgcc gatccccgta ccgaccgcgt aaaaggccga
     9541 aatggacaac gccctcattt ccagcgggaa cacttcgctt acggtgaggt aagccgaact
     9601 cgccgcggca gatgccagga agaatacccc cgaccagcat acggtttgcg tcacggcatt
     9661 gagccaaccc tgcatgaagc caaatccggt cgcggccaag gcgacgccgg acagtccata
     9721 ggtcagcgca atcattctgc gtcgtccgaa ccgatcgaat agcggaccga gcagcacggg
     9781 accgaggaaa ttcgcaatcg cgaacgggaa aatatagcgg cccacctgcg acgccgatac
     9841 gccttgaaaa cgcgtcagga ccagcgcgta agtaaaaaat atcgcgttgt agaagaacgc
     9901 ctgcgacacc atcaatgaca ggccaagcag tgtgcgattc ggatagcggc gcagcaacag
     9961 atgagcaaac tcgctcagcg acggctgtgg ccttacgact atggaaagcg ttctgtcggg
    10021 cggttgcaga gagccatgct gcctggcaac ggccagctca atgccggcaa ctgctcgttc
    10081 agcatcgtcc cggcggccac ggatcaacaa ccagcgcggg ctttcgggca cgtcacggcg
    10141 taccagcaga atcgcaatcg cgagcagtgc gccgagccca aagccgattc gccagcccac
    10201 atccgcatgg aacagcctcg tgtcgagcaa caccaggctc agcccggcgc caacagcggc
    10261 gccgagccag aagctgccgt tgattgcgag actgacccgt ccacgcacgc gtgccggaat
    10321 cagctcatcg attgccgaat tgatcgccgc atattcgccg ccgatgccaa atccagtgaa
    10381 caatcggcac aacaggaacg tcgtgaagtt gtacgaaaag gcgcttgcca gagtggccgc
    10441 cacatagacc gcgagtgtaa gaataaacag ccgcttccgg cccgcagaat cgacgagtcg
    10501 cccgaaaaac aaggcgccga tgacggcacc gccgacataa cccgagccgg cccagccaat
    10561 ttgctcagcc gtcaggccta gcgtgtcggg tcgttccagc acgctgccga tcgaccctac
    10621 cagggtcact tccagcccat cgagcatcca ggcgattccg agcgcaatca ccacgcgcca
    10681 gtgccatgct gaccagggta agcgatcaag ccgggcggga atgttgctgc tcaggcgttc
    10741 ctgcaccatg ttcgctccca caacgaatcc ggcgaattcc cggaatgaga gcgtggcgaa
    10801 tgtggtagtc ccaacagggc gcatcgctcc gaagcggttc gacttgcgac cgcacgatag
    10861 gtttcttacg atcgatcagg ctcgccgcgt gcgcagcacg ccaagcgtca cggcacctgc
    10921 aagcagcccc caaaacgccg aaccgattcc cagcaatgtc acgccggacg cggtcaccag
    10981 aaaggtaatg agcgcagcct cgcgttcttg ttctgccgcc attgcagccg acagaccgtt
    11041 accaatggtc ccgatcaacg caaggcccgc aatggctgca acgagctcat ggggaaacgc
    11101 cgcaaacagc gcgccgacac tcgcgccgaa caggccaatc agcagataaa agaaacccgc
    11161 agccatggac gcgacgtagc ggcgtttggg atcttcttgc gcttcgcttc ccatgcagat
    11221 cgccgctgtg atggcggcca ggttgattgc aaatgcgccg aatggcgcta acagcacatt
    11281 cgccaggccg gtccaaccca cgacgggcga tattggtatc ggataaccgg acgcgcggat
    11341 caccgctaca cccgggacat tttgtgaggc catggtcacg acaaacaacg gcactgccac
    11401 gccgacgagc gcgctaagtg aaaaagcagg cgtcacccaa accggcgtgg ccaattcgaa
    11461 cgtcaccgat tccagatgca acgtgccttg cgcaacagca atcgcgatac cgagcgcaaa
    11521 cgccgcaatc accgcgtagc gcggatgctt gcgccgcatg atcagatacg ccgcgaacat
    11581 cgcaaacgcc atcacgaact gcgctttcat gacaacaaac aattccagtc cgaaacgcaa
    11641 cagcacgccg gccagcatgc cggacgcaat ggaaaccggg atgcgattca tggcgcgttc
    11701 gaaccatcct gagaatcctg ccaaggtgat catcagcgca gacaccagaa acgcgccagt
    11761 ggcctgggcc agcgagattc ccgccgcgga tgtaatgagc atcgctgcgc cgggcgttga
    11821 ccatgctgta acaaccggca tgcgatagcg caaggacaaa gccatgcagg tcacgcccat
    11881 gccgaggccg agagcccaca tccaggaagc gatttcggtg cttcctgccc caacggcgtg
    11941 tgccgcctga aatacgagga ctccagaact cgtgaagcca accagcacgg tcacaaagcc
    12001 cgctacgacc gcagagaggg agatgtcccg tagcagtggc atatcagccc aaatttttgt
    12061 tttgtgcgcg agcgcgcatc atcagaggat ttggacacga ccggtgccgg atgactccaa
    12121 ccatgtccgc gcaactactc gttgccaggc ggattcaagc cactttggcg acttttattt
    12181 gtgcaggatc gcgctgcaag acatcgcgaa tctgcatgag ttcttccacc tgcttgccaa
    12241 actgcagctt ttgctgctcg agctgccgga cgcgttcctg cagcgtattg atgtcgcctg
    12301 cgaccttgcg caggttctca accctgcgct ccagcaactt ctgatgttcc tttagttgca
    12361 cggaaagcgg cgtcaaggcg gaattgaccc aggcatccag ttccaggcgc aattgagtga
    12421 acaattcacg cgcatgtgaa accagtccac tgtaaaaacg gcgaacgaga aaatgcttct
    12481 cggtcatcac attgaccgga tctttgcaga aacgctcggt attctggtgc agcgcatgca
    12541 tgcgcaggat atgacgctcg agattcagcg ccggtggtgt catttttgga aagccgtaca
    12601 gtttgtgaaa ctcgccgtag acgccatcga cgtagttgcg cgtttcggtg gcgaattcca
    12661 gaattttttc ggcctgccgt gaaaatgtat cgaataaggc ctgcatgctg cgcatcagtc
    12721 ccgccgtagt ccaactcccc tcgattgatg caaggctgcg gctcatcatt tcgtcgagac
    12781 gctcttcggc cagcgtgccg agcagcacgt taccctgctt gaacactgtt gtgctggagc
    12841 gcttgtagct ttccatgtgc cgcatgtaat ggcctttgtc ttcttcgagc ttggcaagca
    12901 gcagcttcgc gagatcgcgg ttcttcccgg atagtctgga catctcctgc aactcgcctc
    12961 gcgtactgat gagttgcgct gtggcgctct gcagggacgt ttccagcata ttgccaattt
    13021 cgcgcgacac ggcagcgcgc aatatttgct gtttcgacgg aatgacctct tcggccagca
    13081 gccgctcaag cacttcgatc ctgctccttg ccaacagcgc cgcatcgccg cgtatacgtc
    13141 caatcaaacc cttttgcgca gacaatgcga tgacatgatc gcgcggcaaa cccaatattc
    13201 tggaagtctc gatcagctgg cgttcaatcc cgatttcaat ctggtcgtcg cttttgagat
    13261 cgtcccacat cagatcaatc ttgttgagca cggcaatccg gcgggtcaca tagttctgca
    13321 cgtacttttg ccagacgtcg agatcggact tggtcacgcc ggtgtccatt gccagcaaga
    13381 acagtacggc atgtgcactc gggatcatgg atagcgtcag ctcgggttcg gtgccgagcg
    13441 cgttcaggcc cggcgtatcg agaatgacca ggcctttttt cagcagcgga tgcggatagt
    13501 tgaccaaggc gtggcgccag catggaattt cgaccttgcc gccctgcttc accaccaccg
    13561 tggtcgtcac gaaatcggtt tcatccagca ggccgagcag gctcgcctct tccacggtta
    13621 cggacttcac ctgcgcgagg ctcgacatcg ccgtcgccat ctcttcctga gaggcgaggt
    13681 ttagcttgat cttgacccac tccaccggct tgtgcttcaa cgccgcgatg ctttcgtcgc
    13741 ctttgcgcga ctcaatcggc aacagcttga tatagggttc ttccgccgaa tcgaagaaga
    13801 tctccgtcgg gcacatggtc gtgcggccca cgtctgacgg cagcagccgt tgcttgaaat
    13861 ccgagaagaa catcgcgttg atcaactcgg tctttccgcg cgaaaattcc gcaaggaaag
    13921 cgaggatcat gcggtcgttg cgcaggcttt cgaccaggtc ataaatgcgc agactgcgct
    13981 ggatgtcggc gtggccatgg tcctccagcc agttctggta ggactgaatg caatcgcgca
    14041 attcgtcgcg ccattgctgg tatgccgtta cctgattttc gagatcgctg gtcatgagtg
    14101 cagcagttgt ttcacccgcg ccatgagggt tgcgccctgg ccgtagattt cgacaatttt
    14161 ggtgcgcccg tgactgccac ggcgaatcat aacccgcccg cccggcagat caaacttgtc
    14221 cgcaaggaat cggagcaggc attcgtttgc cttgccttcg acagccggcg cgttaagccg
    14281 caccttgagg tgtccgccat gaagtcccgc gaactcggtt cgcctggcat tgggttgcac
    14341 atgcaaattg agggtcagca ggccacgatc cggatgatat cgataccagg tatcgggcac
    14401 ggtaaagtca acaatcagag catgcgggtg atcgcctgtt ccagcgcact caccggccac
    14461 atcagcaata tctggcagat caccagcacg aacagcggtg aaagatccac tccgcccacg
    14521 ggcgcgacac gacgttggaa tacggacagg aaaggtcggg tcaagctggc cagaaccgag
    14581 gtcgccggtc cacgcggggc aacgaaagac aagatggcct gcgcgaacac cgccaccatg
    14641 agtatgtaga tgaacagctt cagcagattc agcgttgaca acagcataag tccgaccacg
    14701 gccgaaccca cacctggccc gacctggtag cctttcaaag cgagcacact gctgaccaga
    14761 actaattccg tgacccaggc cagcaacagc gtggaaaggt catatcccca caagcccgga
    14821 acgacacgcc gagtcggccg cacgatgaaa tccgtcagtg cggcgaggaa ctgcgacaat
    14881 gggttgcggg cgggcgcacg taccgtttgc aagtaaaaac gcatgagcag agccaacgaa
    14941 aacaggccaa ggaatgtttc cagcaggaat atcaggacct ggttcatgtc gtcggccttc
    15001 gtgatgtgtt tgcgttttca gggtttgcca aagtcgtcgc caagctggct cgatcgacgg
    15061 gctgcggcat gcaccgcttc cacgatcttg ctcttgacct gattcgtttc catggaacgc
    15121 aatgctgctt ccgtagtgcc ggccttcgag gtcacgcgcg cacgcagaac ggctgccggg
    15181 tcgtcactac cgtccgccag cctggcagcg ccagcaaaag tctgaacggc cagctcgtgc
    15241 gcctgtgtct cattgaggcc caactcgagc gcggcctgtt ccagcgcctc aatgaaataa
    15301 aaaacataag ccggtccgct gccggagacc gcggtaaccg catcgatagc cggctcatca
    15361 gccacccaca ccacgccgcc aaccgcgccg agaatatctt gtgcgcagtc cctgtccgcc
    15421 tgaccgacat tggcagcggc gaataatccg gacacaccgg atccaattag cgccggcata
    15481 ttgggcatgg cgcgcacgat gcggtcgtga cctccaagcc agcgcgccaa atcgtaagtg
    15541 cggattccgg ccgcaatgct gatgaccaat tgcccgcgca agtaagctcg cagttccgtt
    15601 gccatctccc gcatttgctg cggtttgacc gccagcagca cgacatcctc cgcaatttca
    15661 ctgctgagca ctgcgctcgc atgcacgcca aatcgctgtt ccaagcgctg gcgcgctggc
    15721 tgcattacat caatggcgcg aagctcgccg ggcttgaaac cccgctgcaa catcccgcct
    15781 atgagcgcgg aggccatgtt gccgccgcct atgaaaagta tgcgcatcgg tttaaagcgc
    15841 agttgtggcc aacgggacgt gcggcgcgga agacactccg caccgcgatc gctttattta
    15901 cttcgtttcc gccatcttct tgacgttctg caagccagcc tgatacgcac cgctgatggc
    15961 tttctttacg ccggcatcat cctgtcccgc cgggggatta tcggccacct tgcgtttgaa
    16021 tttacctttc cagatcagca cggacgtgtc tttggttttc ccggatttga cctggatagt
    16081 agaggaataa tcggccaccg gcagagggga atccgtgatg atccggtagc tgtacttctt
    16141 cttcttgtcg ctgaatccgt ccagttcttc atcgaacttg gctccgccgt cggccatggt
    16201 cagcgtacgc actgcgccga ctttattatt cgcgccggat cttatttcgg tattcgagaa
    16261 agctggatgc catttatgca gaccgttcca gtccttgacc aggccccaga ccttggcaac
    16321 aggcgcattg atatcaacgc tttcggtcac atgcaaatat cccggctttg ccgaccatgc
    16381 cattcccggc aacgccagca gcagagccgc cgctatgacc atcttccttc tgttcattac
    16441 ttttctccct tgttcacgtg agtacgacgt tgatcccggc cggcgtgccg gtcgggctgt
    16501 tcaagcggat accgctgctc ggcgtccggc gggccagtat aggtgcgctc cgccgtcgcg
    16561 ggcaaatcag caatcaggcg taaacggcac tcgctctcca aagatcgcac gcccgacgcg
    16621 gaccagggta gcgccttcag caatggcggc ttccaaatcg tcggacatgc ccatcgacaa
    16681 ggtatccagg ctgatgccct gacggttgat ttcgtcctta agttgccgca cctgccggaa
    16741 gcgcgcccgc tgcacgacaa tatcgtcggt cggctcgggg atcgccatta gacccctcaa
    16801 ctgcaaaccc ggcagctggc tcagggcctg agccagcgcg accgcttctt cggggcggac
    16861 gccgctcttg ctgccttcac cgctgacatt gacctggagg caaatctgca gcggcccctg
    16921 agtggcaggg cgtgccgcgg caagccgttg ggcaatcttg atacggtcca ccgcatgcac
    16981 ccaggtgaag tgttccgcaa tttgtcgcgt tttgttgctc tgaatcggac cgatgaagtg
    17041 ccactccaaa gggaggagca ggggccccgc ttgcggggat gcgaccccgg cggaggagcg
    17101 catgtcgccc tcttgcggtc ctcgttcctt ctgtccaggc gcgtcatgcc actgcaaagg
    17161 gaggagcagg ggcctcgggg atgcgacccc ggcggagcgc atgtcgccct cttgcggtcc
    17221 tcgttcctcc tgtccgggcg caacgccgat cggccgggtt tgagacaaag cctgtatttt
    17281 gccgagtgct tcctgcaaat agttctctcc aaaggcgaat tggccagcgg cgcacgcttc
    17341 tgccacggcg gccggagaga aagtcttgct aacggcaatc agcccaatct cgtcgggtga
    17401 tctgccggcg cccagagccg caacgcggat acggctgcgt acggcttgca agcgggccgc
    17461 tattgttgtc ataattctct tgcattcttc attgtaacca tcgaatgcag cgagcatttt
    17521 tgttctgagc cgaagccgcc ggcataggtt ggtcccattg tttggtcgcc ggagaaccgt
    17581 tgatggacat tgctgagctc ctcgcgtttg tggtcaagaa caaggcatcc gacttgcatc
    17641 tttccgccgg tcttcctccg atgatcaggg tgcatggcga catcaggcgc atcaatttgc
    17701 cgcctatgga gcacaaggat gtgcatgcca tggtttacga catcatgaac gatggccagc
    17761 gcaagttcta tgaagagaac ctcgaatgcg acttctcttt tgcgattcca aacctggccc
    17821 gttttcgcgt caatgccttt gtccagaacc gcggagccgg cgcagtaatg cggaccatcc
    17881 cttccaaggt gctgactctg gaggaactca aggctcccgt catcttcaag gaaatcgccc
    17941 agtacccgcg cggcgtggtg ctcgtgaccg gccccaccgg ttcgggaaag tccaccacac
    18001 tcgcggccat ggtcaacgat atcaacgaaa acgagatggg tcatattctg acggtggaag
    18061 accctatcga gtttgtgcac gaaagcaaaa aatgcctcat caaccagcgt gaagtcggcc
    18121 cgcatacgtt gtcgttcaac aatgccctgc gcagtgcact gcgtgaggac ccggacatca
    18181 tcctggtggg cgaattgcga gaccttgaaa ccatccgcct cgcgttgact gctgccgaaa
    18241 ccggccatct ggtattcggg acgctgcata ccagctcagc cgccaagacg gtggaccgta
    18301 tcatcgacgt atttccggca gcggagaagg aaatggtgcg ggcaatgctg tcggaatcct
    18361 tgcgtgcggt catctcccag acactgtgca agaccaaaga cggcacgggc cgcgctgccg
    18421 cgcatgagat catgatcggc accccggcca ttcgtaacct gatccgcgaa gcgaaaattg
    18481 cgcaaatgta ttccgcaatc cagaccggcc agggcctcgg aatgcagacg ctggaccaga
    18541 acctgactga gctcatcaag cgcaacatca tcacgaaaga agaagcacgc ggcaaagccg
    18601 ctaacaagga cacgttcaac tgacagggac ggcaggcttc acgaatatca ccaggagcag
    18661 agatggaacg agatcaggcc gtcaaattca tacatgaact gttgcgtgcc atggtggcga
    18721 aacgggcgtc ggacctgttt attacggcag gatttccgcc ggctttcaaa ctcgacggaa
    18781 aaatgacgcc ggtatccaat cagagcctga caccccagca cacccaggaa ttggcgcgct
    18841 cgctgatgaa tgacaagcag gcgcaggaat tcgaggccag caaagaatgc aatttcgcca
    18901 tcaacccgcc cggcatcgga cgcttccggg tcaacgcttt tgtgcagcag gcccgggtcg
    18961 ggatcgtgct gagaacgatc acgacggcca ttccaaaaat ggaagatcta gggctgccgc
    19021 ccgtgctcaa agatgtggcg atgaccaagc gcgggctcgt catcctggtg ggtggcaccg
    19081 gttccggtaa atccaccaca ttggccgcaa tgatcggcta ccgaaatgag aacagccacg
    19141 gccatatcat cacgatcgaa gacccaatcg aatacgtgca cgatcacaag aactgcctgg
    19201 tgacccagcg tgaagtcggt gtcgataccg attcgtggga atccgcactg aagaacacct
    19261 tgcgccaggc gcccgacgtt attctcatcg gcgaagttcg agagcgcgag accatggaac
    19321 acgcgatcgc ctttgccgag accgggcatt tatgcatgtg cacgctgcat gccaacagcg
    19381 caaaccaggc attggaccgc atcatcaatt ttttcccgga agaacgccgc ccgcaattgc
    19441 tgatggacct gtcgctgaac ttgaagggcc tggtgtccca gcgattgatt ccactcaagg
    19501 aaacaaaggg ccgtgcagcg gcaatcgaaa ttcttttaaa ttctccgctg atatccgacc
    19561 tcatcttcaa aggggaggtc catgagatca aggaaataat gaagaagtcc agggaaatgg
    19621 gcatgcagac cttcgatcag gcgttgttcg atttgtacga gggcggcaaa atcggctacg
    19681 aagacgccat gcgcaacgcg gactcaatga acgaactgcg cctgcagatc aagctgcacg
    19741 gcaaggaaat gcgcgatcgc gacctcacca ccggcgtgga tcatctgaac attgtgtaac
    19801 gcgaaactgc ggtcgcagcc agaacgcggt taacggctga tacagcccgt ctcaggcgtc
    19861 caactccggc cccttctttt ggcgtcgcat cgcgcccgat accattttga attcgtatag
    19921 ccggcactcg agcgcgccgt tgtacagtgg cgtgcgcctg gacggcgaca ggccaatcag
    19981 tttcggcagg cgcagatcgg ccgtgaacag ataggcacgc cagccagcaa acttctgctt
    20041 g
//
LOCUS       DATMZQ010000359         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3050809, whole genome shotgun sequence.
ACCESSION   DATMZQ010000359 DATMZQ010000000
VERSION     DATMZQ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050809"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VN496_11635"
     CDS             <1..1292
                     /locus_tag="VN496_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17651.1"
                     /translation="NALTHVHNLEIRPSAMTRKFASGDVDPEKIGQQLKVGTVLTGHY
                     LQQDRSMRVTLEAIQVKDNKVIWQSTLTVPAAQLITLQDQLTKKVRRELPLAIGAGGG
                     TTDTTSVPANPDAYDLYLRSVPVDHDPKPNKEGIAMLERAVGMDPSYAPAWEALGRRY
                     YFDAIYSGGGEEGYKRSNAAYERALALEPDRVSAAGFLAQNHVEQGLLQQAYGEVRDL
                     VRRRPENAVAHFSLSYILRYTGMLDEAQRECDKALTIDPGNYNFRSCSFAFFEAGNTH
                     RAKEYLALDPGSEWSNAILVSVLLREGKTQEAREALKQMTDNPTWMKAFLEACMDGKP
                     AAETDKMAEQIEASLLSEKDSELKYHQAGLLAYCGKKPIALRFLQKAVAENYCAYSGL
                     QSDPLLVSLRSEPDFKTLVANAGGCQKKVKESTPGAR"
     gene            1432..>2676
                     /locus_tag="VN496_11640"
     CDS             1432..>2676
                     /locus_tag="VN496_11640"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXF17652.1"
                     /translation="MGGFASRRLAATFLCLVALPLSAKAPAFSNRAGQPLAQDTGISG
                     LKDMLRRLQTTARLMQTVAHPDDEDGGMLTLESRGKGASVLLLTLNRGEGGQNKIGSN
                     LFDVLGVLRTLELLGSGQYYGVEQRFTRVADFGFSKNPQETFDKWNGHDVALGDMVRV
                     IRTFRPDVLVSRFSGTERDGHGNHEASGILTKEAFRAAADPKRFPEQIAEGLLPWQAK
                     KLYSGNVCGFFASTCAAANYTVKLNTGKPDAVIGSSPIEFAMQGLRHQLSQGAANWTV
                     EPGDRYTYYKLVDSVSAPKLDADGHETDFFDGLDTTLLGLATRLGAEESVVPFLRPAL
                     VELDVHIKQASQAVEKDPQTATDPLLKGLQLIRKITAQLQGSRLSPAAGRELMTNLRA
                     KELQFQAAANLALGLVLDATVDS"
BASE COUNT          654 a          691 c          776 g          555 t
ORIGIN      
        1 caaatgcgct cacccacgtc cataacctgg aaatccggcc atcggcgatg acacgcaagt
       61 tcgcttctgg cgatgtggat ccggagaaga tcggccagca attgaaagtg ggaacggtgt
      121 tgacaggtca ctacctgcag caagaccgtt cgatgcgcgt gacgctggaa gccattcagg
      181 tgaaggacaa caaggtcatc tggcagtcaa cgcttaccgt gcccgccgca caattgatca
      241 ccctgcaaga tcagctcacg aaaaaggtcc ggcgtgaatt gccgcttgcg atcggcgctg
      301 gcggcggcac aaccgatacc acgagcgtgc cggcgaaccc cgacgcttac gatttgtatc
      361 ttcgaagcgt ccccgtagat catgatccga aaccgaataa agaaggtatc gccatgctgg
      421 aacgggcggt aggaatggat cccagctatg cgccggcatg ggaagcactg ggtcgacggt
      481 actattttga cgcaatctat tcgggcggtg gtgaggaggg atataagcga tccaatgccg
      541 cttatgagcg tgccttggcg cttgaacctg atcgggtatc agcggccggg tttcttgcgc
      601 aaaatcatgt tgagcaaggg ttgctgcagc aggcgtatgg cgaggtacgc gatctggtga
      661 ggcgtcgccc ggagaacgcg gtcgcgcatt tctccctttc ttatatcttg cgatatacgg
      721 ggatgctcga cgaggcgcaa cgtgaatgtg acaaggctct aaccattgat cccggaaact
      781 acaacttccg ttcctgttcg tttgcattct tcgaggccgg gaatacgcat cgtgcgaagg
      841 aatatttggc attggatccg ggttcggaat ggtccaacgc aattctagtt tcggtgctat
      901 tgagggaagg caagactcaa gaagcgcggg aagcactgaa gcagatgaca gacaatccga
      961 cgtggatgaa ggcatttctg gaagcttgca tggacggaaa gcccgcggca gagaccgata
     1021 agatggccga acaaatcgaa gcgagcctgc tttctgaaaa agactcggag ctgaaatatc
     1081 atcaggcagg actgctggcg tactgcggaa aaaagccgat cgcgctgcgg tttctgcaga
     1141 aggccgtggc cgaaaactac tgcgcatact ccggccttca aagcgatccg ttgcttgtaa
     1201 gcctgcggag cgaaccggat ttcaagaccc tggtagccaa tgctggtggg tgccagaaga
     1261 aagtaaagga gtcgacgccg ggggcacggt gaccgagcat ttctcccttg ctaacgtgcg
     1321 gcagtgcagc cagtccaagt gtaagatagc cgcgtcataa aaatcttgtc tcccagtcct
     1381 caggaaagcc atctgattga tatgcaatca tttataaggg taaggcaatc ggtgggtgga
     1441 ttcgcctcgc gccgactagc cgcgacattt ctttgcctgg tcgcgctccc cctcagcgcc
     1501 aaagcaccag ccttttcaaa tagggccggg cagccactcg cacaagatac aggcatttcg
     1561 ggattgaagg acatgttgcg acgactccaa accactgccc ggttaatgca gacggtcgct
     1621 catccggatg atgaagatgg cggaatgcta acgcttgagt cacgcggcaa aggcgcaagc
     1681 gtgttattgc tcacactcaa tcgtggcgag ggtggccaaa ataaaatcgg cagcaatttg
     1741 tttgatgtgc tcggagtact tcggacgctc gagctgttgg gatcaggcca atattacgga
     1801 gtagaacaaa gatttactcg cgtggctgac tttggatttt cgaagaatcc gcaagagaca
     1861 ttcgacaaat ggaacggcca tgacgtggct ctaggcgaca tggtgcgggt gattcgtaca
     1921 tttcgccccg acgtacttgt ctctcgtttc agcggcacgg agcgtgatgg gcatggcaac
     1981 cacgaagcct cgggcatttt aacgaaagaa gcgttccgag cggccgccga tccaaagaga
     2041 tttcccgaac aaattgcaga aggtctgctg ccatggcaag caaaaaagct ctactcaggc
     2101 aacgtttgcg gattctttgc gtccacctgc gccgcagcca attacacggt taagttgaac
     2161 acgggcaaac ccgatgcagt gatcggcagt tcaccgattg agttcgcgat gcaaggacta
     2221 cgtcatcaac tttcgcaagg ggctgcaaat tggacggttg agcccggcga ccgatacacg
     2281 tattacaagc tggtcgattc agtctccgca ccgaagcttg atgcggatgg gcacgaaaca
     2341 gatttctttg acggcttgga tacgacgtta ctcggtctcg caacgcggct cggtgcagaa
     2401 gaatcggtag ttcccttctt gcggcccgcg ctcgtcgaac tagacgtgca catcaaacaa
     2461 gccagtcagg cggtagagaa agacccccag accgcgactg atccgctgct gaaggggttg
     2521 caactgatcc gcaaaatcac tgcccagctc cagggctctc gcctgagccc agcggcgggc
     2581 cgcgaattga tgacgaattt gcgagcgaag gaattacagt ttcaagctgc tgcaaatcta
     2641 gcgttgggtc tagtgctcga cgcgaccgta gatagt
//
LOCUS       DATMZQ010000360         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_289204, whole genome shotgun sequence.
ACCESSION   DATMZQ010000360 DATMZQ010000000
VERSION     DATMZQ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289204"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1186)
                     /locus_tag="VN496_11645"
     CDS             complement(263..1186)
                     /locus_tag="VN496_11645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXF17653.1"
                     /translation="MHSVKQPVLFALLASCLILAVPGYAAEPKAKETADTKPAPKTEN
                     KADAKQKNVAIVNGIGIPRSRLEFVAKSQLAQAQSQQGQPGQPPLQDTPEFRDNLREI
                     LITREILYQEAVKRKLDKNPDYQTQLDLAKQQIILAVLVDDMSKKLTPTDADLRKEYE
                     RVKSERSATDAGKEYKSRHILLKDEETAKQVIADLDAGGDFATIAKEKSEDPGSKEQG
                     GDLEWSTAQAYVQPFGEALQSLKKGERTKEPVKTNFGYHIIELEDVRTVPFPEFDQVK
                     PQIQQQLATKLRDDYIAELRAKAKVQKIDPK"
     gene            complement(1247..1528)
                     /locus_tag="VN496_11650"
     CDS             complement(1247..1528)
                     /locus_tag="VN496_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HXF17654.1"
                     /translation="MNTEQLIRERLAQLAPESLEVFDDSHEHVGHVGAKEGGGHYQLV
                     IVSGQFAGKNVVARHRLIYEALSDLMRTRIHALAIRAYAPEEMGTSAKG"
     gene            complement(1531..1833)
                     /locus_tag="VN496_11655"
     CDS             complement(1531..1833)
                     /locus_tag="VN496_11655"
                     /inference="COORDINATES: protein motif:HMM:NF008473.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXF17655.1"
                     /translation="MLYAIIGEDVPNSQAKRAAARPAHLKRVDELIGAGKLVLGGAFP
                     AIDSIDPGPAGMTGSLIVAEFNSLEEAKAWINADPYVTEGVFAKVIVKPYRKVFPN"
     gene            complement(1833..2372)
                     /locus_tag="VN496_11660"
     CDS             complement(1833..2372)
                     /locus_tag="VN496_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HXF17656.1"
                     /translation="MKFLFDIFPVLLFFVAFKFWGIYTATAVAIGATMCQVAWSWLRH
                     KKVDTMLWVSLSIIVVFGGATLWLHDETFIKWKPTVLYWLFGVILLGSELFFGRNLIR
                     VMMAKQLTLPATVWRTLNLSWAGFFLLMGAANLYVAFHFPTETWVNFKLFGGTGLMLV
                     FIVVQGLMLSRHIEHEEKK"
     gene            2484..2960
                     /locus_tag="VN496_11665"
     CDS             2484..2960
                     /locus_tag="VN496_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXF17657.1"
                     /translation="MAQVKLVNYAEASPEVRAVFDDIMQTRNTDYINNIWRALASNPP
                     VLKRFWSQMKEVMVKPSRLDPLTKELIYLAVSVNNNCEYCITSHTFAARKHGMDDAML
                     TEFNEIVALANAGNRLTMGLQVEPDERFKPKHPQNAWSVKGKPASGKKPKSPPARS"
     gene            2964..3626
                     /locus_tag="VN496_11670"
     CDS             2964..3626
                     /locus_tag="VN496_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXF17658.1"
                     /translation="MEFERVVRERYSCRQYQKTPVARESIRRILELAQQTPSWCNCQP
                     WQVLLVTGEALEQFRLALHAHAAQGLPARPDFPFPTRYEGLYRERRKICGVQLYQALG
                     IGREDKSAAAQQSLENFRFFGAPHVALITTEQDLGVYGAVDCGLYVANFMLAAQNFGV
                     ASIAQAALASYPDFVRGHFGLPVHRKLVCGISFGYAEPVHPINSYRTARATEQEATIW
                     VD"
     gene            complement(3623..4063)
                     /locus_tag="VN496_11675"
     CDS             complement(3623..4063)
                     /locus_tag="VN496_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HXF17659.1"
                     /translation="MRLVLDTNVWVDWLVFDDWSIAPLKAAQRARQVQIIANEPCLEE
                     LARVLAYPEFALTGAQRADCFEAAGRVIVRHDAPKQSRHKALPRCSDPDDQKFVELAH
                     AARAGWLVTRDKALLQLKPRLHAAGFRVCSPAEWSTAFLLPMEY"
     gene            complement(4070..4897)
                     /locus_tag="VN496_11680"
     CDS             complement(4070..4897)
                     /locus_tag="VN496_11680"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXF17660.1"
                     /translation="MSTSAKVTSSASLRHTTVVNEPIARSAGGPAGTTLEKVRVSKLM
                     SERGLCSRREADEYIERGWVYVDGVRVDELGTRIDPAQNITLDKRAQNISRITVLLNK
                     PIGYVSGQAEQGYRPAITLIVPDSRFAGDNALPRFEREHLKGLAPAGRLDIDSTGLLV
                     FTQDGRIAKLLIGEDSTIEKEYLVRVEGALDGAGLKLLNEGLSLDGVPLKPAKVSWQN
                     RDQLRFILREGKKRQIRRMCELVGLRVIGLKRVRIGRIVLGDLPAGQWRYLHDHEKF"
     gene            complement(4990..>5350)
                     /gene="acnA"
                     /locus_tag="VN496_11685"
     CDS             complement(4990..>5350)
                     /gene="acnA"
                     /locus_tag="VN496_11685"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXF17661.1"
                     /translation="SSRDWAAKGTQLLGVRAVVAKSFERIHRSNLVGMGVLPLQFKGG
                     DSVDALQIHGDETFDISGLGGEIRPQQDVTLVIRRPDGSTREVKLLLRIDTPIEVDYY
                     RHGGILPYVLRELVAAA"
BASE COUNT         1085 a         1571 c         1520 g         1174 t
ORIGIN      
        1 acggatcgcc agaaggcatc accggactta ctacgccgga tggacgcttt acggtcgtca
       61 tgccgcatcc tgagcgcgtg ttccgcacca tccagaattc ctggcatccg gcatcctggc
      121 gggaagacag tccatggatg cgaatatttc gtaatgcccg gcgctgggtc gattgaaaac
      181 ctgacgcgac tgacgcaata acaaacagta caaacagaaa agggcgctgc gtgcgccctt
      241 tttgctgata tcgcaacagc aattacttgg gatcgatctt ctgaaccttc gccttcgcgc
      301 gcaactctgc aatgtaatcg tcgcgcagct tggtcgccag ttgctgctgg atctgcggct
      361 tgacctggtc aaactcgggg aaaggcacgg tcctcacatc ttccagctcg ataatgtgat
      421 atccaaaatt ggtcttgacc ggctccttgg tgcgctcgcc tttcttcaag gactggagcg
      481 cttccccgaa aggctggaca taggcttgcg ccgtgctcca ttccaggtct ccgccttgtt
      541 ccttgctgcc agggtcttcg gatttttcct tcgcgatggt cgcgaaatcg ccgcccgcat
      601 ccagatcggc gatgacttgc ttggcggtct cttcgtcttt caacaggata tgccgtgatt
      661 tgtattcctt gcccgcatcg gtggcgctac gctcgctttt cacccgctcg tattccttgc
      721 gcaagtccgc atccgttggc gtcagtttct tgctcatgtc atcgaccagc acagccagga
      781 taatttgctg cttggcgaga tcgagctgcg tctgataatc agggtttttg tccagcttgc
      841 gcttgacggc ttcctggtaa agaatttccc gggtgatgag gatttcgcgc aggttatcgc
      901 ggaattccgg tgtgtcctgt aaaggcggtt ggccgggctg gccctgctgg gattgagctt
      961 gggccaattg ggatttggcg acgaactcca ggcgcgagcg cggaatgcca atgccattga
     1021 cgatcgccac gttcttctgc ttggcgtccg ctttgttttc cgttttgggt gccggcttgg
     1081 tatccgcagt ttcttttgcc tttggttctg ccgcatatcc tggcacagcc aggataaggc
     1141 agctagccag tagcgcgaac aggacgggtt gtttgaccga gtgcattgca gttcctttgg
     1201 tgacgggagt tgaactctgg aatgggtttg tgctcttctt cagcgttcaa cccttggctg
     1261 aagttcccat ttcttccggg gcgtaggcgc gaatcgcaag cgcatggata cgggtacgca
     1321 tcaggtccga caatgcctcg tatatcagcc ggtggcgtgc aacgacgttc ttgcccgcga
     1381 actgcccgga tacgatcacg agctgatagt gtccgccgcc ttccttcgcg ccgacatggc
     1441 cgacgtgctc gtggctatcg tcgaagactt caagcgactc cggagcaagt tgcgcgagcc
     1501 gctcgcgaat caactgttcg gtgttcatag tcagttcggg aataccttcc gatacggctt
     1561 tacgatcacc ttggcgaata caccctcagt aacgtaaggg tcggcgttga tccatgcctt
     1621 cgcttcttcg agcgaattga attccgcgac aatcaggctg ccggtcatgc cggccgggcc
     1681 tgggtcaatg ctgtcaatcg ccggaaaggc accacccagg acgagtttgc ccgcaccgat
     1741 cagttcgtca acacgcttca ggtgcgccgg tctcgccgcg gcgcgcttgg cctggctgtt
     1801 tgggacgtcc tcgccaatga tggcatacag cattatttct tttcctcgtg ttcgatatgc
     1861 cggctcagca tcaatccctg taccacgatg aataccagca tcaagcccgt tccaccgaac
     1921 aacttgaaat taacccaggt ttcagtcggg aaatggaaag cgacatacaa attggcggcg
     1981 cccatgagca ggaagaaacc cgcccaactc agattcaggg tacgccagac tgtggccggc
     2041 aaagtaagct gctttgccat catgacccgg atcaggtttc taccgaaaaa caattctgaa
     2101 ccgagcagga tgaccccgaa aagccagtat agcaccgtcg gtttccactt gatgaaggtc
     2161 tcatcgtgca accacagcgt agcgccgccg aagaccacga tgatggacag gctaacccac
     2221 agcatggtgt cgactttctt gtggcgcagc caggaccatg ccacctggca cattgtggcg
     2281 ccaatggcga cggctgttgc cgtgtagatg ccccaaaact tgaaggccac gaaaaacagc
     2341 agcaccggga aaatatcgaa caggaacttc atgatggtca ggggaaagag gcgggcatta
     2401 tggactcacg gtggtacggt gtccaacggg catcgggtaa gatggccgcc tcgtttcgag
     2461 tcgcattcgc ggagggagtt tcaatggcgc aggtaaaact ggtgaactat gccgaagcat
     2521 cgcctgaagt gcgggcagtg ttcgacgaca tcatgcaaac gcgcaacacc gattacatca
     2581 acaacatctg gcgcgcactg gcttccaatc cgccggtgct gaagcggttc tggtcgcaga
     2641 tgaaagaagt catggtgaag ccctcgcggc tggaccctct caccaaggaa ctgatctatc
     2701 tggcggtgag cgtgaacaac aattgcgaat attgcatcac gtcgcatacg tttgcggcac
     2761 gcaagcacgg catggacgat gcaatgctga ccgagttcaa cgagatcgtg gcgcttgcca
     2821 atgccggcaa ccggctcacc atgggtctgc aggtcgaacc ggatgaacgc ttcaagccaa
     2881 aacatccgca gaatgcgtgg tcggtcaagg gcaaaccggc gagcggcaag aaaccgaagt
     2941 ctccgcctgc gcggagctag cggatggaat tcgagagggt tgtccgggaa cgctacagct
     3001 gccgtcaata ccagaagacg ccggttgcgc gggaaagcat ccgtcgcata ctcgagctgg
     3061 cgcagcaaac accttcctgg tgtaactgcc agccttggca agttctgctc gtgaccgggg
     3121 aagcgctgga gcagttccgg ctcgcactgc acgctcacgc ggcccaagga ctgccggctc
     3181 ggcccgattt tccttttccc acgcgctatg aaggtctgta ccgcgaacgg cgcaagattt
     3241 gcggtgtgca gctataccag gcgctcggta tcggccgtga agacaagtcg gctgctgcgc
     3301 agcagtcgct ggaaaatttt cgctttttcg gcgcgccgca cgttgcactg attaccaccg
     3361 agcaggatct gggcgtgtac ggtgccgtgg actgcggact gtacgtggcg aatttcatgc
     3421 tggccgcgca gaatttcggc gtggcttcaa ttgcgcaggc tgcgctggct tcgtatccgg
     3481 atttcgtacg tggccacttc ggccttccag ttcaccgtaa attggtttgc ggcatttcat
     3541 tcggctatgc ggagcccgtg catccgatca acagctaccg taccgcacgc gctaccgaac
     3601 aggaagcaac catctgggta gactagtact ccattggtag caggaatgcg gtcgaccact
     3661 ccgcaggcga gcagacgcga aagccggctg cgtgcaatcg cggcttcagt tgaagcagcg
     3721 ccttgtcgcg ggtgacaagc caccccgcac gtgccgcatg tgccagttcg acaaatttct
     3781 ggtcatcggg atcgctgcag cgaggtaacg ccttgtgtcg ggattgcttg ggcgcatcgt
     3841 gcctgacgat gactcgcccg gcagcttcga aacaatcagc gcgctgtgcg ccggtcagcg
     3901 cgaattccgg ataagccaac acgcgggcca gctcttcaag gcacggttcg ttggcaatga
     3961 tctgaacttg ccttgcccgc tgtgctgcct ttaaaggtgc gatggaccaa tcatcaaaca
     4021 ccagccagtc gacccaaaca ttggtatcca gtaccagccg catggccgtt cagaattttt
     4081 cgtgatcgtg caggtaacgc cactgccccg caggcaaatc gcccagtacg atgcgaccga
     4141 tacgcacgcg cttcaggccg ataacacgca agccgaccaa ctcgcacatg cgccgaatct
     4201 gccgcttttt tccttcgcgc aggatgaagc gcaattgatc ccggttctgc cagctgactt
     4261 ttgcgggttt caacgggaca ccgtccagac tcagaccttc attgagcagc tttaggcctg
     4321 cgccatcaag cgcgccctca acccggacca gatactcttt ctcgatcgtg gaatcttcgc
     4381 cgatcagcag tttcgcaatg cgtccgtcct gcgtgaacac gagtagtccc gtggaatcaa
     4441 tgtcgagccg gcctgccggg gctaatcctt tcagatgctc tcgttcaaac cgcggcaagg
     4501 cgttgtcgcc ggcaaaccgg ctgtccggca cgatcaaagt aatggccgga cggtatcctt
     4561 gctctgcctg gccggacaca taaccgatcg gtttattcag cagcaccgta atgcgcgaaa
     4621 tgttctgggc gcgtttatcg agcgtaatgt tttgtgccgg atcgatgcgg gtgccaagct
     4681 cgtccacgcg cacgccgtcg acgtataccc agccgcgctc gatgtattcg tcggcttccc
     4741 ggcgggaaca cagtccgcgc tccgacatga gcttggacac ccggactttc tcaagcgtgg
     4801 tacccgcggg cccgcctgcg gaacgtgcaa ttggttcatt tacgactgtg gtatggcgca
     4861 atgaagccga cgatgtgact ttggcagagg tcgacatgcg tgaccgcgcg cgccgtgccc
     4921 tcgtcgatgg actggcgtcc ggccggctgt gggtgcctgc cggcttgcgc agaatgctgc
     4981 gtccgccggt caagccgcgg cgaccagttc gcgcagcacg taaggcagaa ttccaccgtg
     5041 ccggtaataa tccacttcga tcggcgtatc gatgcgcaga agcaatttca cttcacgcgt
     5101 cgatccgtcc ggccgcctga tgaccagggt cacatcctgc tgcggacgga tttcgccacc
     5161 caggccgctg atgtcgaacg tttcgtctcc gtggatctgc agcgcatcca cgctgtcgcc
     5221 ccctttgaac tgaagcggca gcacacccat tccaaccaga ttggagcggt gtatgcgctc
     5281 gaagctcttg gccaccacag ccctgacacc cagcaattgg gttcctttgg ccgcccagtc
     5341 gcgtgatgat
//
LOCUS       DATMZQ010000361         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4087275, whole genome shotgun sequence.
ACCESSION   DATMZQ010000361 DATMZQ010000000
VERSION     DATMZQ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4087275"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VN496_11690"
     CDS             complement(<1..615)
                     /locus_tag="VN496_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXF17662.1"
                     /translation="MSAVRNPKHSGDFHVADLGLADWGRKEIRIAETEMPGLMAIREE
                     YAATRALRGARISGSLHMTIQTAVLIETLQALGAEVRWASCNIYSTQDHAAAAIASAG
                     TAVFAVKGETLAEYWDYTHRIFEWPDGMHSNMILDDGGDATMLLHLGTRAETDASALH
                     NPGSEEESALFAAIKAKLKQDPTWYSTRLDKIRGVTEETTTGVHR"
     regulatory      complement(671..744)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(812..1993)
                     /gene="metK"
                     /locus_tag="VN496_11695"
     CDS             complement(812..1993)
                     /gene="metK"
                     /locus_tag="VN496_11695"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXF17663.1"
                     /translation="MRDFLFTSESVSEGHPDKVADQISDAVLDAILTQDKFARVAAET
                     LVNTGLVLMAGEITTHAVVDFQQVARDTIKRIGYDNTDYGIDYRGCAVLVAYDKQSPD
                     IARGVDKAQDDPLNQGAGDQGLMFGFACDETPVLMPLPIYLAHRLVERQSELRRDGRL
                     PWLRPDAKSQVTVRYVDGKPDAVDTVVLSTQHAPEISLEKIREAVIEDIIKPVLPKNL
                     IKGEVKYLVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAGLATKCQIQVSYAIGVAKPTSVMVTTFGTGRIDDEKIAQLIQK
                     HFDLRPKGILAMLDLLRPIYQKTAAYGHFGRDEPEFSWENLDRVETLKADAGIRATAV
                     A"
     gene            2082..>2805
                     /locus_tag="VN496_11700"
     CDS             2082..>2805
                     /locus_tag="VN496_11700"
                     /inference="COORDINATES: protein motif:HMM:NF006487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXF17664.1"
                     /translation="MLIRLLRQLAKLPLSWLHVVGGALGWLVYHVSDDYAKRLRENLS
                     QSGVWQDQKDYERILRRNIFESGKAGTELIAVWFRPVASAVQLIVRAEPIGLVEAAEK
                     RGRGVIYLTPHLGCFDAAALWAAQRRPITVLYRPPKIKQLQPLIEAGRGRDRVQLAPA
                     NLGGVRLLLKALKRGEAVGILPDQVPAEGEGVWADFFARPAYTMTLAGKLADATGATI
                     LMAAAVRLQQGAGFVIHFTQFNG"
BASE COUNT          583 a          820 c          798 g          604 t
ORIGIN      
        1 gcggtgcaca ccggtcgttg tctcttcggt gacgcccctg atcttgtcca ggcgcgtgga
       61 ataccaggtc ggatcctgct tgagcttggc cttgatggcc gcaaacaatg cgctttcttc
      121 ctcgctaccc gggttatgca gcgcggacgc gtcagtctcc gcgcgcgtgc ccagatgcag
      181 caacatcgtt gcgtcgccgc catcgtccag aatcatgttc gaatgcatgc catctggcca
      241 ttcgaaaata cgatgggtgt aatcccagta ctctgccagc gtctcgcctt tgacagcaaa
      301 caccgccgtg ccggccgagg cgatggccgc ggcggcatgg tcctgtgtcg aataaatatt
      361 gcaggaagcc cagcgcacct cggcgcccaa cgcctgcagc gtctcgatca agacggcagt
      421 ctgaatggtc atgtgcaaag acccgctgat gcgagcgccg cgtagcgcgc gggtcgccgc
      481 atattcttcg cggatcgcca tcaggccagg catttctgtt tcagcaatgc ggatttcttt
      541 gcggccccaa tcggccaggc ccaggtcggc gacatggaaa tcaccggagt gcttcggatt
      601 acgtacagca gacatcgaca ttctcctttg acagagcgcc gaaatgagaa cggcgctcac
      661 aaccagttga gggttgtgag cgccgttggt tcgtttgctg agcctggcct tccattgcga
      721 aaggtcgcag cgctcctcag cgaaaggcca ttataagcca gcgcgccggc ctcgaacagc
      781 ggccgctgtg ctgttcctta cggttcagag actaggcgac ggccgttgcc ctgatgccag
      841 cgtcggcttt caacgtctct accctgtcca ggttctccca cgaaaactcg ggctcgtccc
      901 ggccaaaatg gccgtaggcc gcagtttttt ggtagatcgg acgcagcaga tcgagcattg
      961 ccaggatacc tttcgggcgc agatcgaaat gcttctgtat taattgcgca attttttcgt
     1021 cgtcgatcct tccggtaccg aacgtcgtga ccattacgct ggtcggcttt gccaccccga
     1081 tggcataaga cacctggatc tggcacttgg tggcgaggcc tgcggccacg atatttttcg
     1141 ccacgtaccg cgcagcatag gctgcggacc gatccacctt ggatggatcc ttgccggaaa
     1201 atgcaccacc gccatgaggc gcagcgccgc cgtaggtatc gacgatgatt ttgcggccag
     1261 tcagcccgca gtcaccttgc ggtccaccaa caacgaatcg accggtgggg tttaccagat
     1321 acttgacttc ccctttgatc agattcttcg gcagtaccgg cttgataatg tcttcgatca
     1381 ccgcttcgcg aattttttcc agtgaaattt ccggtgcatg ctgcgtggaa agcactacgg
     1441 tatcgacagc atccggcttt ccatccacat aacgaacggt gacctgcgat tttgcgtccg
     1501 ggcgcaacca gggcaggcgt ccatcgcgac gcaattccga ttggcgttca accaggcggt
     1561 gcgccagata aattggcagc ggcatcagta ccggcgtttc atcgcaggca aaaccgaaca
     1621 tcaggccctg gtcacctgca ccctgattca acggatcgtc ctgcgccttg tccaccccgc
     1681 gcgcaatatc aggcgattgc ttgtcgtaag caaccaaaac cgcacagccc cggtaatcta
     1741 tgccgtaatc ggtattgtcg tagccgatac gcttgatcgt gtcacgcgct acttgctgga
     1801 aatcgactac ggcatgcgtt gtaatttctc ccgccatgag cacgagaccg gtattgacca
     1861 acgtctcggc tgcgactcgg gcaaatttgt cctgagttaa aatggcatcc agcacggcat
     1921 ccgaaatttg atccgcgact ttatccggat ggccttcaga gaccgactcg gatgtgaaca
     1981 ggaaatctct cattcgaaaa gtctgggcga gttcatgagg ggcggtcagg atagcaaaaa
     2041 gccatcaaaa gaacaaaacg gacgatccaa caagcaagca catgctcata aggctgctgc
     2101 ggcaactggc aaagctgccg ctctcctggc ttcatgtggt gggcggcgca ctgggctggc
     2161 tcgtctacca tgtttcagat gattatgcga aacggctgcg cgaaaatcta tcgcaaagcg
     2221 gtgtctggca ggatcaaaag gactatgagc gaatcctgcg gcggaatata ttcgaatcgg
     2281 gcaaggcagg gaccgaactg attgcggttt ggttccgtcc ggtcgcatcc gcggtacaac
     2341 tgatcgtacg cgctgaaccg atcggactag tggaggcggc cgaaaagcgc ggtcgcggcg
     2401 tcatttacct cacgccgcat cttggatgct tcgatgccgc cgcgttatgg gcggcgcagc
     2461 ggcgaccgat caccgtgctc tatcggccgc ccaagataaa acagcttcaa ccgttgatcg
     2521 aggcgggacg cgggcgagac cgggtgcagc tcgcgcctgc aaatcttggt ggtgtgaggt
     2581 tgctgttgaa agcgctcaag cgcggtgaag cggtcggcat tcttccggat caggtgcccg
     2641 cagaaggaga aggcgtgtgg gccgattttt ttgcgcgccc cgcctacacg atgacattgg
     2701 caggcaaact ggctgacgcc actggcgcca ctattctgat ggccgccgca gtccgccttc
     2761 aacagggcgc aggttttgtg attcacttca cgcaatttaa cggcg
//
LOCUS       DATMZQ010000362         3506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2704275, whole genome shotgun sequence.
ACCESSION   DATMZQ010000362 DATMZQ010000000
VERSION     DATMZQ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704275"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VN496_11705"
     CDS             complement(<1..300)
                     /locus_tag="VN496_11705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF17665.1"
                     /translation="MKRLRHPSPYLLLTLTALFWAANWVIGRAMRNDMPPIAMGFWRW
                     SIALLILLPVAAPELRRKWNIVRSNWLTLTLLGLLGAVAFNTLIYVGLQYTEATNG"
     gene            complement(515..1915)
                     /locus_tag="VN496_11710"
     CDS             complement(515..1915)
                     /locus_tag="VN496_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXF17666.1"
                     /translation="MSFSDLGLAPELLRAIADQGYTEATPVQAKAIPAILDGQDVMAA
                     AQTGTGKTAGFTLPMLQRLKEFANTSVSPARHPVRALILTPTRELAAQVQDSVRTYGK
                     YIALRSAVVYGGVHIDPQIAELRAGVEILVATPGRLLDHLGQKTVNLSQVQFLVLDEA
                     DRMLDMGFLPDIKRIISVLPVKRQSLLFSATYSDDIRGLAKQFLIDPISIEVARRNSA
                     TETVTHLVYKVESAAKRDLLTHLIKSRDMRQVLVFCNTKIGANRLAYKLDKDGVGAAA
                     IHSDRTQGERMLALAEFKEGKTKVLVATDIAARGLDIEQLPFVINFDLPGSPEDYVHR
                     IGRTGRAGMTGEAISLVSDQELDDLAAIEKLLKLKLPVQDAGRFERERASVPAQAGAR
                     RRERGAAPRHDDHRRSERERRPSAASTDPLFSKPYTPSSPQTPLVQPAASTRGGPKKQ
                     IAALLLPPVPAEKQDA"
     gene            complement(2082..3245)
                     /locus_tag="VN496_11715"
     CDS             complement(2082..3245)
                     /locus_tag="VN496_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXF17667.1"
                     /translation="MTKANRFDKILGIGMLAALVIGCLLVLLPFVTALLMAAILTYST
                     WPVYVRLREAVGGRRSLAAGLMMLGACIVLIAPFVFVAMSLADSIAELVEASRKAFEH
                     GPPELPAWLSGLPLVGENLARYWNTLTHDGTRLLDELMGLIAPARSVLLAGGGLLFAG
                     LLQLGLSVLVAFFLYRDGEAAAARIERMAVRIGGARGTHLLHVAGHTVVSVVYGILGT
                     ALSQGLVAGIGFVIAGVPGATLLGMATFFLSVVPVGPPLVWVPAALWLFFYGSTGWAI
                     FLAVWGVLAISMVDNVLKPIIISKGSNLPFMLGFLGVLGGAATLGFIGIFIGPTLLAV
                     GYRMFSEWVDSEERASQPAPDVSAARELPVAPAASASAEPLRENDRFRALSKK"
     gene            complement(3282..>3506)
                     /locus_tag="VN496_11720"
     CDS             complement(3282..>3506)
                     /locus_tag="VN496_11720"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17668.1"
                     /translation="FKQARELDEMPARIQALETEQAQLDARLADPLLYSKPASEIRQL
                     QQRHAAIEEELTACLRRWEELEALRDHAGA"
BASE COUNT          748 a         1047 c         1030 g          681 t
ORIGIN      
        1 gccgttggtc gcttcggtgt actgcagtcc aacatagatc aaggtattga aggccactgc
       61 ccccagaagc ccaagcaatg tgagggtcag ccagtttgag cgcacgatat tccatttgcg
      121 gcgcaactct ggagcagcca ccggaagcag gatcaacagc gcaatgctcc agcgccagaa
      181 tcccattgca atcggcggca tgtcattgcg cattgcacgc ccgatgaccc agttggcagc
      241 ccagaacagg gctgtcaagg tcagcagcag ataaggagat ggatgacgca agcgtttcac
      301 gaagaccgtg aatatcgtaa aggccgtgaa tgtcgtgaat gcgtgactcg aaaaactcta
      361 cttcaattga acaatggttc ctgcagtcag ggcctacaga gattggatca accgccaatc
      421 tggttgtgca gtggaattac gaattcacga tattcacgat attcacgata tttatggtcg
      481 gagaccggta tcccgcgttc gcggggcacg gcctttatgc gtcctgcttt tccgcaggca
      541 ctggaggcag gagtagcgct gcaatctgtt tcttcggtcc gccgcgcgtg gatgcagcag
      601 gttgaaccag cggtgtttgg ggagaggacg gagtgtatgg tttggagaac agcggatcgg
      661 tgcttgcagc agaagggcga cgttcacgtt cacttcgtcg atggtcgtcg tggcgaggcg
      721 cggcgccccg ctcgcgtcgg cgcgcgccag cttgtgccgg cacggaagcg cgttcccgct
      781 caaatcggcc agcatcctgc accggcagtt tgagtttgag caatttttcg attgcggcaa
      841 gatcgtccag ttcctggtcg gacaccagcg agatcgcctc gccggtcatg ccggcacgac
      901 cggtgcggcc aatacgatgc acataatctt ccggcgaacc aggaagatcg aagttaatca
      961 cgaaagggag ttgctcgatg tccaggccgc gtgccgcaat atcggttgcg accaggacct
     1021 tcgtcttgcc ttccttgaac tccgccagcg caagcatgcg ctcgccctgg gtacgatcgc
     1081 tatgaattgc agcagcgccg acgccatcct tgtccagctt gtaggccagg cggtttgcgc
     1141 caatcttggt attgcagaac accagtacct ggcgcatgtc gcgcgacttg atcaggtgcg
     1201 tcagcagatc acgcttcgct gcggattcga ccttgtagac cagatgcgtg accgtctcgg
     1261 tggctgaatt gcggcgcgcc acttcgatgc tgattggatc gatcaaaaac tgttttgcca
     1321 gcccgcggat atcgtccgag tacgttgctg aaaacagcag gctctggcgt ttgaccggca
     1381 gtaccgaaat gatgcgcttg atatccggca aaaatcccat gtcgagcatt cgatccgcct
     1441 cgtccagtac caggaactgt acctgcgata ggttcacggt tttttggcca aggtgatcga
     1501 gtaaccgccc cggcgtcgcg accagaattt ccacgccggc gcgcagctcg gcgatctgcg
     1561 gatcaatgtg cacgccaccg tataccacgg cagagcgcaa cgcgatgtac ttgccgtacg
     1621 tcctgacgct atcctgaacc tgtgcggcca gctcgcgagt gggcgtaagt atcagcgcgc
     1681 gcaccggatg gcgcgcaggc gaaacactgg tgttggcaaa ctctttcagg cgctgcagca
     1741 ttggcagcgt aaaaccggcg gtcttgcctg tgccggtctg ggcagcggcc atcacgtcct
     1801 ggccgtccag gatggctgga atcgccttgg cctggaccgg cgtggcttcg gtataacctt
     1861 gatctgcaat tgcacgcaac aactctgggg cgagccccag atcggaaaac gacattgact
     1921 ttcaactcct gcgccgccca ccttaaggca gcaccgttag attggatgat tagtgccggc
     1981 gaatgccgca cctgaattga ccgcgcatgc tacagcataa gtcgcgaatc tgaaagatga
     2041 tttggcttgg cgcaattcca cccatcgact tggttctctg gttatttttt ggacaatgca
     2101 cgaaatcgat cgttctcccg cagcggttcc gcactggcgg aggcagccgg cgcgaccgga
     2161 agctcgcggg cggcggacac gtccggtgca ggttgggagg cgcgttcctc gctgtctacc
     2221 cattcactga acatgcggta gcccacggca agcaaggtcg gaccaatgaa aattccgata
     2281 aagcccagcg tggcggcccc gccgagcacg ccaagaaagc cgagcatgaa tggcaggttg
     2341 ctgcctttgc tgataatgat tggcttcagc acgttgtcca ccatgctgat cgcgagcacg
     2401 ccccataccg ccagaaaaat cgcccaaccg gtggaaccat agaagaacag ccagagcgcg
     2461 gccggcaccc acaccagggg cggaccgact ggcaccaccg acaggaaaaa cgtcgccatc
     2521 ccgagcaacg tggcacccgg tacgcccgcg atgacgaatc ctattccggc gaccagcccc
     2581 tgcgacagcg ccgtgcccag aatgccatag accacgctga cgaccgtatg ccccgccaca
     2641 tggagcaggt gcgttccgcg agcacctccg atgcggacag ccatgcgctc gatgcgcgcg
     2701 gcggcggcct ctccatcgcg gtacagaaaa aaagctacca gtaccgacag gcccaattgc
     2761 agcaagccgg caaacaacaa tccgcccccg gcgagcaaca ctgatctggc cggagcgatc
     2821 aagcccatca gctcgtccag caggcgcgtg ccgtcatgcg taagcgtgtt ccagtagcgg
     2881 gcgagatttt cacccaccag tggcaggccg ctcagccatg ccggcagctc gggcggtccg
     2941 tgttcgaagg ctttgcgaga tgcttcaacc agctccgcga tgctatcggc aaggctcatg
     3001 gccacgaaca cgaatggcgc gatcagaacg atgcatgcgc ccagcatcat cagacccgct
     3061 gccaatgatc tgcggcctcc tacggcttcg cgcagccgca catagaccgg ccaggtggaa
     3121 taggtcaata tcgccgccat caacagcgcg gtgacaaatg gcagcagtac gagcaggcag
     3181 cctatgacca gcgcggccag catgccgata ccgagaatct tgtcgaagcg gttggccttg
     3241 gtcatcgaag caaggttacg ttaagctgag tcatcgcgac atcaagcgcc tgcgtggtca
     3301 cgcagggctt cgagttcttc ccagcggcgc aggcaggcag tgagttcttc ttcaatcgcc
     3361 gcatggcgct gctgcaattg ccggatttca cttgctggct tgctgtacag caacggatcg
     3421 gcgaggcggg cgtcaagctg tgcctgttct gtttccagag cctggatgcg tgccggcatt
     3481 tcgtcaagct cccgggcctg cttgaa
//
LOCUS       DATMZQ010000363         3731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2017570, whole genome shotgun sequence.
ACCESSION   DATMZQ010000363 DATMZQ010000000
VERSION     DATMZQ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017570"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VN496_11725"
     CDS             <1..711
                     /locus_tag="VN496_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXF17669.1"
                     /translation="DPQAHLSYVCGSSVGNADDSIFSFYQSIKPLTQLIRPATGGWLV
                     IPAHVELSKVDTQFGKGPNILNRLNSGIVKENLNTGRPIVLDCCPVLGVLSLSAIFAS
                     DRVLIPVSTDYLAVHSVFQVEKTLRALEHVLKKRVMRRYVMTRFDSRRNMSHQIYDEL
                     KERLGEDVCDTRITENVSIAESPASKKDVFAHSPDSRGALDYEALLEELVQVGFIERR
                     EREVITESPVRDFLRSPV"
     gene            complement(683..943)
                     /locus_tag="VN496_11730"
     CDS             complement(683..943)
                     /locus_tag="VN496_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17670.1"
                     /translation="MSAPIPPAPQQMLKFGFRIRTRSGLPVENLVVHARDQAEAERKI
                     IQMYQHCEIIECKQMVSPGREEGTDLEGIISLISRQANEENP"
     gene            complement(946..1470)
                     /locus_tag="VN496_11735"
     CDS             complement(946..1470)
                     /locus_tag="VN496_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HXF17671.1"
                     /translation="MIYSLGDRKVITRGDYWVADNATVIGSVVLEHNASVWFNSVVRG
                     DNDLITIGDGSNIQDSCVLHTDAGVRLTLGRNVTVGHQVMLHGCTIGEGSLIGIKSTI
                     LNHAKIGRHCLIGAHTLITEGKEIPDRSLVVGSPGKVVRQLTDTEVEQLLENALHYVT
                     NFQRFRLDLNVQPG"
     gene            1586..2680
                     /locus_tag="VN496_11740"
     CDS             1586..2680
                     /locus_tag="VN496_11740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HXF17672.1"
                     /translation="MSAVTAVKTSGETDIAKVLGADADKLLSHVCKGIPKESLHLPGP
                     DFVERVFVPSDRTPPVIRSLQQMFDHGRLSGTGYLSILPVDQGIEHSAGASFAKNPAY
                     FDPENIVRLAIEGGCNAVASTLGVLGSCSRKYAHRIPFLLKVNHNEFLTYPNKYDQIL
                     FGSIKQARDMGAVAVGATIYFGSPESARQIVEVAKAFEMAHERGMATVLWCYLRNPAF
                     KSDKDYHVSADLTGQANHLGVTIQADIIKQKLPENNGGYNAISSKENPYGKVDKRMYT
                     ELATDHPIDLTRYQVANCYMGRAGLINSGGASGENDMAEAVRTAVINKRAGGMGLISG
                     RKAFQRPLKEGIAILNAIQDVYLSRQVTIA"
     gene            3123..>3731
                     /locus_tag="VN496_11745"
     CDS             3123..>3731
                     /locus_tag="VN496_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17673.1"
                     /translation="MQHLQSLLIWPDRILLSLLVLLVIAVPFLYAARSPMHALIKSAS
                     RALSNPLRLGARWLADASIRLRNRNRQVLFAHGSQEVRQTVEREFERVTTLVARDLEG
                     YPVLQRKLMDEIVRIEEDYKKTGEVPLPPPEWVKAIESLAKIKASGDGVVERILVEIR
                     NSLGGIYDKVMSEYRGAYQDRHRILKGFLPFWRSVSQTLTQVD"
BASE COUNT          819 a         1030 c         1041 g          841 t
ORIGIN      
        1 gatccgcaag cccatctgtc atatgtttgc ggcagtagcg tcggcaatgc cgatgattcg
       61 attttcagtt tctaccagag catcaagccg ctgacacagc tgatacgtcc cgcaactggc
      121 ggctggctgg tcattcccgc acacgtcgag ctgtccaagg tcgataccca attcggcaaa
      181 gggcccaaca tccttaaccg actgaacagc ggaatcgtca aggaaaacct gaacaccggc
      241 cgccccatcg tgctggactg ttgcccggtt ctcggcgtgc tgagcctgag cgccatcttc
      301 gcttccgatc gcgtgctgat tccggtttca acggattatc tcgccgtgca tagcgtgttc
      361 caggttgaga agaccttgcg ggcgctcgaa cacgtgctca agaagcgcgt gatgcgccgc
      421 tacgtcatga cgcgttttga cagtcgtcga aacatgtcgc accagattta tgatgaactc
      481 aaagagaggc tcggcgagga cgtttgcgat acgcgcatta ccgagaatgt cagcatagcg
      541 gagagccccg cgtcgaaaaa agacgtcttc gcgcattcac ccgacagccg cggcgcgctg
      601 gattacgagg cgttgctgga agagctggtg caggtgggct ttatcgaaag gcgcgaacgc
      661 gaagtgatca cggagtcgcc tgtcagggat tttcttcgtt cgcctgtcta gaaatcagcg
      721 agatgatgcc ttcgaggtcg gtgccttcct cgcgacccgg tgaaaccatt tgcttgcact
      781 cgatgatctc gcagtgctga tacatctgga taatcttgcg ttctgcttcg gcctgatcgc
      841 gggcatggac caccaggttt tccaccggca agccgctgcg agtgcggatc cgaaatccga
      901 acttcagcat ctgctgtggc gcaggcggta tcggtgcgga catgactatc ccggttggac
      961 gttcaaatcc agccgaaagc gctgaaaatt ggtgacgtag tgcaaggcat tctccaggag
     1021 ctgctccact tccgtatcgg tgagttgccg caccactttc ccgggcgaac ccaccaccag
     1081 tgaccgatcc gggatctcct tgccttcggt gatcagtgtg tgggcgccga tgaggcagtg
     1141 tctgccgatc ttggcatggt tcaggatggt cgatttgatg ccaatcaggc tgccttctcc
     1201 tatggtgcaa ccgtgaagca tgacctgatg tccgaccgtc acattcctgc cgagcgtaag
     1261 ccggacgcca gcgtcggtgt ggagcacgca actatcctga atgttcgagc cgtccccaat
     1321 ggtgatgagg tcattgtctc cccgcactac actgttgaac cagacgctgg cattgtgctc
     1381 caacaccacg gagccgatga ccgtagcgtt atccgcaacc caatagtcgc cgcgagtaat
     1441 gacttttctg tcgcccagag aataaatcat gctgttttta ttgcgggact ggatgagtca
     1501 ttataaggga cgcttaacgc cagcaccgcg ctggaagata atactgccga gataaatact
     1561 gttcaccgtt caggagatcc ccaatatgtc cgctgtcaca gccgtcaaaa caagtggtga
     1621 aaccgatatc gccaaggtgc ttggtgccga tgccgataaa ctgttgagcc atgtctgcaa
     1681 gggcataccg aaggaatcgc tgcacttgcc cgggcccgat ttcgtcgaac gtgtgtttgt
     1741 gccgtcggat cgcaccccgc cggtcattcg cagtctgcag cagatgttcg accacggccg
     1801 gctcagcgga accggttact tgtccatttt gcctgtggat cagggtatcg agcactcggc
     1861 cggtgcgtcg tttgctaaaa atccggctta tttcgatccg gaaaacattg tccggctggc
     1921 catcgagggc ggctgcaatg cggtcgcctc gactctcggc gtgctgggtt cgtgctcgcg
     1981 caaatatgca caccggattc cttttttgct caaggtaaat cacaacgagt tcctgaccta
     2041 tcccaacaaa tacgaccaga tcctgttcgg aagcatcaag caggcccgcg acatgggggc
     2101 agtcgccgtc ggtgccacga tttatttcgg gtcaccggaa tccgcgcggc aaatcgtcga
     2161 agtcgcgaaa gcattcgaaa tggcgcatga acgtggaatg gcaacggtgc tgtggtgtta
     2221 cctgcgcaat cccgctttca aatccgataa ggactatcac gtgtctgctg acttgacggg
     2281 ccaggccaac catttgggcg ttacgattca agccgacatc atcaagcaga agttgccgga
     2341 gaataacggt ggctacaatg caattagcag caaggaaaat ccgtacggta aagtcgacaa
     2401 gcgcatgtac acggaacttg ccactgacca tccaatcgac ctaacacgct atcaggttgc
     2461 gaactgctac atgggccgcg cggggctcat caactcgggc ggcgcatcgg gcgagaacga
     2521 tatggctgaa gctgtgcgca ccgccgtgat caacaagcgt gctggcggca tgggtctcat
     2581 ctcggggcgc aaggctttcc agcggccgtt gaaggaaggt atcgcgatcc tgaatgcgat
     2641 ccaggatgtc tatctgtcac ggcaagtgac tatcgcctga agtttaaatc tttactgcaa
     2701 taaaggcggc cgctcggccg cctttttatt ttgaaatcga aaagcatctt gcgcgtttgg
     2761 ttgcgatttt gtctcacgat tagaccgcaa aggcatcgca tgtctctctt tttgttaacc
     2821 cgcatatcag agcctaatcg cagctgctcg atttgatacg ttgccgtgcc aggtgatgca
     2881 tcgcgcaaaa atacgcaaat ttcggcaccg ctcgtcgctt catagcgaca tgttcttgta
     2941 gagggaaatc gctcttccgc cgtgcgccgc caattaacca cgcaataaca gggccgtaac
     3001 cacatcgcat atatgacgca caacttattg tgcaactgcg ttgctgctgg cccgcgcgtt
     3061 gcacatacca actgggtaaa tgggttcttt tgccgatgta aaactaccct aaggggggag
     3121 aaatgcagca tctacaatct ctattgattt ggccggaccg catactgttg tcattgctgg
     3181 tcctgctggt gattgccgtg ccgtttttgt atgcggctcg ttcaccaatg catgcgttga
     3241 tcaaaagcgc atcgcgggcg ctatccaatc cgctgcggct gggcgcgcgc tggctcgccg
     3301 atgcttccat tcgactgcgt aatcggaatc gtcaagtgct gtttgcgcac ggcagtcagg
     3361 aagtaaggca gaccgtagaa cgcgaattcg aacgtgttac cacgctggtc gcgcgcgatc
     3421 tcgaaggcta cccggttctg cagcgcaagc tcatggacga gatcgtccgt atcgaagagg
     3481 actacaagaa aaccggggaa gtgccgttgc cgccaccgga gtgggtgaaa gcgatcgaat
     3541 cgctcgccaa gatcaaggcg tccggcgacg gcgtggtcga acggatcctc gtggaaattc
     3601 gtaattcgtt gggtggcatc tacgacaaag tgatgtcgga ataccgcgga gcctatcagg
     3661 acaggcaccg catcctgaag ggcttcctgc cgttctggcg ttcagtcagc cagacgctca
     3721 ctcaggtgga t
//
LOCUS       DATMZQ010000364         5152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3396675, whole genome shotgun sequence.
ACCESSION   DATMZQ010000364 DATMZQ010000000
VERSION     DATMZQ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3396675"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VN496_11750"
     CDS             complement(<1..318)
                     /locus_tag="VN496_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17674.1"
                     /translation="MIFRPVILMLLAALTLSAQTLPRLADGKPNLQGIWQVRNQASVD
                     LQDHAARTGVPAGKSVVEGGTIPYQPMAAAKKLENFANRQTADPLAKCFLPGVPRVMY
                     LEYP"
     gene            446..1765
                     /gene="mgtE"
                     /locus_tag="VN496_11755"
     CDS             446..1765
                     /gene="mgtE"
                     /locus_tag="VN496_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXF17675.1"
                     /translation="MDTELQITSNLLESWPALTPEERVEAFERIPRAEADDFFLELPP
                     RDQAALILALQVGERRIWMRLLAPDDATDVIQEAPEEDRASLIAMLDESARREVAALM
                     AYQEDDAGGLMSPRFARLRPEMTIDEAIAYLRRQAQSGLVETVHYSYVLDSEQRLLGV
                     VTFRRLFSGQRDQIVRDIMRTEFTKASEEMDQEQLAQLFTEKHLLAIPIVDASGHMKG
                     IVTVDDIVDVVSEEASEDIQKIGGTEALDGPYLEVGFLQMVRKRGGWLAALFLGEMLT
                     ATAMGHFEKEIATATVLALFIPLIISSGGNSGSQATTLVIRAMALGEVRLRDWWRVVH
                     REIASGFTLGCMLGTIGYVRIILWQSIHWYDYGPHYQLVALSVAFSLVGVVLFGTLAG
                     STLPFILRLLKLDPASASAPFVATLVDVTGLIIYFTVASIILHGTLL"
     gene            2060..3031
                     /locus_tag="VN496_11760"
     CDS             2060..3031
                     /locus_tag="VN496_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF17676.1"
                     /translation="MSRGVEIRELMKNFVLTRWSGLRRRVSDVVAVDGISLSVPPGQS
                     VAFIGPNGAGKSTTIKMLTGILHPTSGEVSVLGFIPWRDRRRLAHQIGAVFGQRSQLW
                     YHLPPGDTFDLLSRIYSLKREEFVRTRNVLIERFNLGSFMNTPVRKLSLGQRMRAEVA
                     ASLLHRPKVLFLDEPTIGLDVIARQELRNLICEWNRNDGTTIFLTSHDAGDIEHVAER
                     VIVINHGQVVVDDNVAAVRRKYLGAKILRVQFHEVPRPVLLPGVTVLCSSEYEWKLQI
                     DTAITPVRRVTSAVLDAGSVADIAVEDPPLEEVIARIYCESRREVPS"
     gene            3028..3825
                     /locus_tag="VN496_11765"
     CDS             3028..3825
                     /locus_tag="VN496_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17677.1"
                     /translation="MKQAEKYLWIGCTAARSNLAYAGEVIYRSTFIAVILYIFMRLWT
                     VVYAGAGSQRIGGLTRPQMLWYLVITEAILMSTPRVAAEVDDDVRTGRLAVHLIRPLS
                     YVCGHLSRAMGDRVVRFGINLAVGAVIAFILVGPIPMSLPGLAMFLAVLPAAFLLDFL
                     GMFLIGLCAFWLESTSGLVLIYSRAVMMFGGMFLPLEIYPAAMQPALRVLPFASMVSA
                     PGRMFVNPSFRHFGEAIVIQGCAVVVFAIVVAMVQSIALKRLFANGG"
     gene            3827..4627
                     /locus_tag="VN496_11770"
     CDS             3827..4627
                     /locus_tag="VN496_11770"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HXF17678.1"
                     /translation="MWRSVLDYLHLAVAYMRINIRAQLEYRGAFFSQIAAMFINDGVW
                     VVFWMFFFTRFPVLHGWNLKDVLSLWAITTAGYGLAYGVMGNAHRRLAPAIVQGELDL
                     WLLHPRAVLPHLLLGSTIPSAWGDALFGYVVYLGFIRPDAARAMMFVALSVVVAVVFV
                     GLGVMTGSLSFYVGNASTLSDQWRDTSITFATYPPTLFEGKVKLLLFTLIPAGFISYL
                     PVEALRSLSIANLFVAFTGALAVLAAGVAMFYHGLRRYESGNLISING"
     gene            complement(4642..>5152)
                     /locus_tag="VN496_11775"
     CDS             complement(4642..>5152)
                     /locus_tag="VN496_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17679.1"
                     /translation="HYGRERIGADRELDSWAFAYDFSVPLQSRIRLRGEGFVGSNLIP
                     FQGGVLQGVAVFNPGGGAPIQFNKIGSGGGWGELIIRATSDNKNVFYVGVGTDDPRDR
                     KLLSNSTRAKNTVSWASYFRKLTDNVTLAFEWSNWQFRTKTFTSGLPTGNGPGGRGNV
                     FNLALAYQF"
BASE COUNT          967 a         1409 c         1542 g         1234 t
ORIGIN      
        1 cggatattcc aaatacatga cgcgcgggac gccgggcaag aagcatttcg ccagcggatc
       61 ggcggtctgc cggttcgcga agttctccag tttctttgcc gcagccatcg gctgataggg
      121 aatcgtgccg ccctcgacca cactcttgcc tgccggcact cccgttcttg cggcatggtc
      181 ctgcagatcg accgacgcct ggttgcgaac ctgccaaatt ccctgcagat tcggtttgcc
      241 atccgccaac ctaggcaggg tctgcgccga cagcgtaagt gccgccaaca gcattaggat
      301 taccggcctg aaaatcatat gcatctcctc aaattgctct cgcgattcga ccatgaaacg
      361 cgcaacttgg tcaagcaact ctcttgaatc actgtagctg tgagatcgag ccctcacctt
      421 ataatcaatt tgcggtagaa gagtcatgga tactgaactt caaatcacca gcaatttgct
      481 ggagtcctgg ccggcgctga cacccgaaga acgcgtggaa gctttcgagc gcattccgcg
      541 cgccgaggcc gatgactttt ttctggaatt gccgccccgc gatcaggccg ctctcatcct
      601 ggctttgcag gtcggtgaac ggcggatctg gatgcggctg ctggctccgg acgacgccac
      661 cgacgtgatc caggaagcac cggaggaaga tcgcgcgtct ctcattgcga tgctcgacga
      721 atccgcgcgc cgcgaagtcg ctgcattgat ggcctatcag gaagacgatg ccggtggatt
      781 gatgagtccg cgattcgcac gtctgcgacc ggagatgacc atcgatgaag cgatcgcgta
      841 cctgcggcgg caggcgcagt cgggtctggt ggaaaccgtt cactactcct acgtgctcga
      901 ttcggaacag cgtctgctgg gagtcgtgac gttccgccga ttgttctccg gccagcgaga
      961 tcagattgtc cgcgacatca tgcgcacgga attcacgaag gcatccgaag agatggatca
     1021 ggaacaactc gcacagcttt tcaccgagaa acatctcctc gcgattccga tcgtggatgc
     1081 ttcgggccac atgaagggta ttgtcaccgt tgacgacatc gtcgatgttg tgtcggaaga
     1141 agccagcgaa gatattcaga aaatcggcgg caccgaggca ctggatggtc catatctcga
     1201 agtgggattc ctgcaaatgg tccgcaagcg cggtggctgg ctggccgctc tgttcctcgg
     1261 ggaaatgctg acggcgactg cgatggggca cttcgagaaa gaaattgcca cggcgacagt
     1321 tctggcgctg ttcatcccgc tgatcatcag cagcggtgga aactccggat ctcaggccac
     1381 gacgctggtc atccgggcga tggcgcttgg agaagtccgc ttgagagact ggtggcgtgt
     1441 ggtgcaccgc gaaattgctt cgggctttac gctcggttgt atgttgggga ccatcggtta
     1501 cgttcgaatc attctctggc agtcgattca ctggtacgac tacggtcctc actatcagct
     1561 cgtggcattg tcggttgcct tcagtctggt cggcgtcgtt ctattcggta ctcttgccgg
     1621 atccacgctg ccattcatcc tgcggttgct gaaactggat ccggccagcg catccgctcc
     1681 atttgtagcc acgcttgtcg atgtgaccgg cctcatcatc tatttcacgg ttgcgagcat
     1741 cattcttcac ggcactctgc tttagttgcc accgcgagga atttgttttc agccctgccg
     1801 ttttgggttt aaactacctg ccttatgttc ggacgaacca cattcgcttt ctcaacgccg
     1861 catcttcctg taaaggatcg accggtcggc atgtgcgcgc ttgccatgtc gattggacgc
     1921 tggccaacca tctgagaaag cgggagcggc cttcccgctc ccgaacctcg aagaaatttc
     1981 aaagtaacaa ttagggtttt tgggtaagcc gctcccgggg aatgttgcgt cctctaacgc
     2041 ttgtcgttta ggaggtccca tgagtcgtgg agtggaaatt cgagaactga tgaagaattt
     2101 tgtcctgacg cggtggagcg gtttgcggcg aagggtgtct gacgtcgtcg ccgtcgatgg
     2161 tatcagcctc agcgtgcctc cgggccagtc ggtcgcattc atcggaccca atggcgctgg
     2221 aaaatccacg accatcaaga tgcttaccgg aatcctgcat ccgacctccg gcgaagtctc
     2281 ggttcttggg ttcattccgt ggcgtgaccg gcgcaggctg gcgcatcaaa tcggcgcggt
     2341 gttcgggcag cggtcgcaat tgtggtatca cctgccgccc ggagacacgt ttgacttgct
     2401 gtcgcgtatc tacagcctca agcgcgagga gttcgttcga actcgcaacg tgctgatcga
     2461 gcgattcaat ttggggtcgt ttatgaacac gccggtgcgc aaactctcgc tcggtcagcg
     2521 tatgagagcg gaagttgcgg caagcctgtt gcaccgtccg aaagtgttgt tcctggacga
     2581 gccgaccatc gggctcgatg tgatcgcccg gcaggaattg cgcaatctga tttgcgaatg
     2641 gaatcgcaac gatggcacga cgatctttct gaccagtcac gatgccggcg atatcgagca
     2701 cgtggccgag cgcgtgatcg tgatcaatca cggccaggtg gtcgtcgacg acaatgtggc
     2761 ggccgtgcgg cggaagtatc tcggcgcgaa gattctgcgt gtgcaattcc atgaggtgcc
     2821 ccggcccgtg ctgttgcccg gtgttacggt gctttgcagt tccgaatatg aatggaaact
     2881 gcagatcgat acggccatca ctccggtgag gcgcgtcacc tccgccgttc tcgatgccgg
     2941 atccgtagcg gacattgccg ttgaagaccc gccgctggag gaagtaatcg ctcgaattta
     3001 ttgcgaaagc agaagggaag tcccgtcatg aagcaagcgg aaaagtattt gtggatcggc
     3061 tgcaccgcgg cgagatcgaa tctggcttat gccggagaag tgatttatcg cagtacgttt
     3121 attgcggtca ttctctacat cttcatgcgg ctgtggaccg tcgtctatgc gggtgcgggt
     3181 tcgcagcgca tcggaggcct gacgcgcccg cagatgctgt ggtacctcgt catcaccgaa
     3241 gcgatactca tgtcgacgcc tcgtgtcgca gccgaagtgg atgacgatgt gaggacagga
     3301 cggctggcgg ttcacctcat acgcccactc tcttacgttt gcgggcacct gagccgggcc
     3361 atgggcgatc gtgtggtccg atttgggatc aatcttgctg tgggtgcggt catcgcgttc
     3421 atcctggtag gcccgatacc catgagcctg ccgggactgg caatgttcct ggccgttctg
     3481 cccgcggcat tcctgctgga tttcctggga atgttcctga tcggcctttg cgcgttctgg
     3541 ctcgagagta cctccggact tgtcctgatt tattcgcgcg cggtcatgat gttcggcggc
     3601 atgttcctgc cgttggaaat ctatccggcc gcgatgcagc cggcgctccg ggtgctgccc
     3661 tttgccagca tggtgagcgc gcccggacgc atgtttgtga atccgagttt ccggcacttc
     3721 ggcgaagcca tcgtgatcca gggctgcgcg gttgtggtct ttgccatagt ggtggcaatg
     3781 gttcagtcca ttgcgctgaa acgcctgttt gccaatggag gttgagatgt ggagatcggt
     3841 tctggattat ttgcacttag ccgttgcgta tatgcgcatc aacatccgcg cccaactgga
     3901 ataccgcgga gcattttttt ctcagatcgc cgcgatgttt atcaatgatg gcgtatgggt
     3961 ggtgttctgg atgtttttct ttacgcggtt tccggtgctg cacggctgga atctgaagga
     4021 tgtcctgtcg ctttgggcta taacgacggc ggggtatggc ctggcgtacg gcgtgatggg
     4081 gaatgcgcat cggcgtctgg cccctgccat cgttcagggt gagctggatc tgtggttgct
     4141 gcatccgcgc gcggtgctgc cgcatctgct gcttggcagc acgattccca gcgcatgggg
     4201 cgatgctctg ttcgggtatg tggtctacct gggtttcatc agacccgatg ctgcgcgcgc
     4261 gatgatgttt gttgcactgt ccgttgtggt tgcagtggta tttgtggggt tgggcgtgat
     4321 gaccggcagt ttaagtttct acgtgggcaa tgcgagcacg ctttcggacc agtggcggga
     4381 cacctcgatc acgtttgcga cgtatcctcc gacgctgttc gaaggaaagg tgaagctgct
     4441 cctgttcaca ctgataccgg ctggatttat cagttacctg ccggttgagg cgctgcgatc
     4501 cttgtcgatt gcaaatctgt ttgtcgcatt cacaggagct ctggcggtgc ttgccgccgg
     4561 tgttgccatg ttctatcacg gcctgcggcg gtacgaatcc gggaacttga tttcgataaa
     4621 cgggtgaaca aggccagatc tctagaactg atatgccagg gccagattga agacattgcc
     4681 gcggccgcca ggaccgtttc ctgtgggaag accgctggta aaggttttgg tcctgaactg
     4741 ccagttcgac cactcgaatg ccagagtcac gttatccgtc aacttgcgga aatagctggc
     4801 ccacgatacg gtatttttcg ctcgggtcga gttcgacaac agcttgcgat ctcgcggatc
     4861 gtcggttcca acgccgacat agaacacgtt tttattgtcg ctggtagctc ggatgatcag
     4921 ttcgccccag ccaccgccac tgccgatctt gttgaattgg atcggtgctc caccgcccgg
     4981 attaaaaacc gcgacgcctt gaagcacccc gccctgaaac ggtatcaggt tactgccgac
     5041 gaagccttcg ccgcgaagtc gaatgcgtga ttgcagtggc acgctgaaat cgtaggcaaa
     5101 tgcccaggaa tcgagctctc tgtcggcgcc gattcgttca cggccatagt gg
//
LOCUS       DATMZQ010000365         4746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4980510, whole genome shotgun sequence.
ACCESSION   DATMZQ010000365 DATMZQ010000000
VERSION     DATMZQ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4980510"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VN496_11780"
     CDS             complement(<1..1492)
                     /locus_tag="VN496_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXF17680.1"
                     /translation="MTDLQRQMDFDADVETRVLLLAPTARDGEITCRILANAGMVCCP
                     VRNLHRLSEELRIGAGAVLLTDEAFSATGAEELRAVLDSQPPWSEVPMVVMMPGALDS
                     AAAQRMMKALRNATILERPALMRSLVSAMQGAIRGRIRQYQIRDQVDAIRQAEQKARE
                     LQLQLELAVEASELGTFHCEMPQGRIVWNDRCKTHFWLSPDAEEITFEVFYSLLHPDD
                     RERTRQAVEETLSKGKQYDVEFRTVSADGRIRWVRAAGRTFYDMTGNAVRFDGTTRDI
                     TQEKLGAEERRMLLDSERAARQEAERVSAVKDEFLATLSHELRTPLNAIFGWTQLLMA
                     GKDDPQTLSHALGVIDRNVRLQTQLIEDLLDVSRIISGKIRLDIQKVDVAEVINAALE
                     SVRPTADAKGIRLEKKLGHLRDMLSGDSGRLQQVLWNLLNNAIKFTDTGGTVSITAER
                     VNCDVEISVRDTGHGISPEFLPNLFARFSQADSSTQRKYGGLGLGLS"
     gene            complement(1494..2969)
                     /locus_tag="VN496_11785"
     CDS             complement(1494..2969)
                     /locus_tag="VN496_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HXF17681.1"
                     /translation="MTDSKGLERCSTGIAGLDDILGGGLPRRRLYLVEGDPGAGKTTL
                     SLQFLRDGAKMGEKCLYVTLSETREELAAGAHSHGWTLDGIDVVEMIADEAELDSEAQ
                     VTMYSQSEVELATMIEKILDGVAKVNPQRVVFDSLSELRLLAQSSLRYRRQILALKSF
                     FAGRQCTVVLLDDHTSEGSDLQLQSIAHGVISLEQHAPLYGAARRRLRVVKLRGSDFR
                     GGFHDFSIRQGGLLVYPRLVAAEHGKSFTRGPIKSGIQELDNLLGGGPDRGTSTLIMG
                     PAGTGKSTIAIQYAVAAAQRGDHASIFAFDESLETLEARMNALGIKFTEGRNAAQISL
                     RQVDPAELSPGEFVHLVRESVERDHAKLIVIDSLNGYLLAMPDEHYLINQLHELLTYL
                     GRRGVTTIMVAAQHGLLGSNMVAPVDTSYLADMVVLLRFFEYAGKVKKAVSVLKKRSG
                     PHEETIRELRFSNEGIALSEPLQQFRGILTGVPVETAPRGD"
     gene            3219..4652
                     /locus_tag="VN496_11790"
     CDS             3219..4652
                     /locus_tag="VN496_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF17682.1"
                     /translation="MSGFAEYEQYDALGLAELVRRNEVTPGELLDAAIERVEARNPAV
                     NAVTMKLYDYGRKAIADGLPDGPFRGVPFLMKDLTSPVAGVPMTRGSKYFADTPPSAI
                     DSEHVRRLKKAGLVIFGRTNTCELGLSLTCEPQLHGPTRNPWNLAHISGGSSGGAAAA
                     VAARMLPIAHASDGFGSIRAPAACCGLVGLKPTRSRNTFAPFAGEGLGGVSTEHAVSL
                     SIRDSAALLDATRGAGPGDPYVAPPPSRPYLQEAGADPGKLKIAWTSAAPNGATIDAA
                     CLRVLAQTVRLCEQLGHDVEECDPKIDREAVVPTFLTLAAANTVVNLSTHPTAGRPAR
                     DSEVERITRLTAKMGESISAADYVRATQMAHRLGRQMATFHTGWDVLLTPGLSSAAVK
                     LGWLDMMMDDLQEYWRRVFEFSPFTVWFNLTGQPALMLPLDQTLGGMPLAVQAVARFG
                     DEATLIRLGAQLEAARPWFARKPAIAA"
BASE COUNT          945 a         1422 c         1384 g          995 t
ORIGIN      
        1 tcgagagtcc cagtcccagt ccgccgtact tgcgttgcgt cgaactatcc gcctggctga
       61 agcgcgcgaa caggttcgga agaaattccg gactgatacc gtggcccgtg tcgcgaacgc
      121 tgatttcgac atcacaatta acgcgctcgg cagttatgga taccgtccca ccggtatcgg
      181 taaatttaat ggcattgttc agcagattcc agagcacctg ctgcagccgg ccagaatcgc
      241 cgctcagcat atctcgcaga tgccccagtt tcttttccag ccggatgcct tttgcatcgg
      301 cggtcggcct tacggattca agtgctgcgt tgatcacttc cgccacatcg actttctgga
      361 tatcgagccg tatttttccg gaaatgatcc ggctgacatc gagcagatct tcaatcagct
      421 gcgtttgcag gcgcacattg cgatcgatca cgcccagcgc atgcgaaagt gtctgcgggt
      481 cgtccttgcc ggccatcaac aattgcgtcc agccaaagat ggcgttgagc ggggtccgga
      541 gttcatgact caacgttgcc agaaattcat ccttcactgc gcttacccgc tcggcttcct
      601 ggcgcgcagc gcgttcgctg tccaacagca tgcgccgctc ttccgcgcca agcttttcct
      661 gtgtgatatc gcgggtcgta ccgtcgaagc ggactgcgtt gccggtcatg tcgtagaagg
      721 ttctgccggc agcgcgcacc cagcgtatgc gtccatcggc ggatacggtc ctgaattcga
      781 cgtcgtattg cttgcccttg gacagggtct cttcgacggc ttgacgggta cgttcacgat
      841 catcgggatg cagcagggaa taaaaaacct caaaagtgat ttcttcggcg tccggactga
      901 gccagaaatg ggttttgcag cgatcgttcc aaacaattct gccctgcggc atctcgcagt
      961 gaaaggtgcc cagttctgag gcctccacgg ccaattcgag ttgcaattgc agttcgcggg
     1021 ctttttgttc agcttgccgg atggcatcca cctgatcgcg gatctggtac tggcggattc
     1081 gaccgcgaat cgccccttgc atggcgctga ccagcgaacg catgagcgcg ggccgctcca
     1141 atatcgttgc gttgcgcagc gccttcatca tgcgctgggc cgcggccgag tccagcgccc
     1201 cgggcatcat gaccaccatc ggcacttctg accacggtgg ctgactatcc aggacagccc
     1261 gcagctcttc cgcaccggtc gccgaaaatg cctcgtcggt gagaagcact gcacccgcgc
     1321 cgatgcgcag ttcttcgctt aaccgatgca gatttctgac cgggcaacag accatgccgg
     1381 cgttggccag gatccggcag gtaatttcgc catcccgcgc agtcggagca agcaataaca
     1441 cccgcgtttc gacgtccgca tcgaaatcca tctgccgctg caggtctgtc acgctagtca
     1501 ccgcgaggag ctgtttcaac cgggacaccc gtgaggatgc cgcggaactg ctgcagtggt
     1561 tcgcttagcg cgataccttc attgctaaag cgcagctccc gaatcgtttc ttcgtgcgga
     1621 ccactacgtt ttttcagaac cgagacggcc ttcttgactt tgccggcata ttcgaaaaaa
     1681 cgcagcagca cgaccatgtc cgccagatag ctggtatcga ccggcgctac catgttggat
     1741 cccaggagcc cgtgctgggc tgcgaccatg atggtcgtga cgccgcgccg gcccagatat
     1801 gtcaacaatt cgtgaagctg gttgatcagg taatgctcat ccggcatggc gaggagatag
     1861 ccgttcaagc tgtcgatgac gatgagtttt gcatggtccc gttcaacgct ctcgcgcacc
     1921 aggtgcacga attctcctgg cgacagctca gccggatcga cctgacgcaa ggaaatctgc
     1981 gctgcgttgc gtccttccgt aaatttgatc cccagtgcat tcatgcgcgc ctccaaagtt
     2041 tcaaggcttt cgtcgaaggc aaatatgctg gcgtgatcgc cgcgctgggc tgcggccacc
     2101 gcatactgaa tggcaatcgt ggattttccc gtgccggcag gacccataat taatgtgctg
     2161 gtaccgcgat cgggcccacc tccaagcaaa ttatcgagtt cctgaattcc acttttgatg
     2221 gggcccctcg tgaaagactt cccgtgctcg gcagcgacca accgcggata gaccagcagc
     2281 ccgccttgcc tgatggaaaa atcgtggaag ccgccgcgga aatccgaacc gcgcaatttc
     2341 acgacccgca gtcgtctgcg tgccgctccg tacagcggcg cgtgttgttc cagcgagata
     2401 acgccgtgtg caatgctctg cagctgcaag tcggagcctt ccgaagtatg gtcatcgagc
     2461 aataccaccg tgcattggcg ccccgcgaaa aatgatttga gcgcgaggat ctgacgccgg
     2521 taacgcaagg agctttgcgc aagcaggcgc aattccgaca gcgaatcgaa caccactcgc
     2581 tgcggattca ccttcgccac gccatccaag atcttttcaa tcatggtcgc gagttcaact
     2641 tccgactgcg agtacatggt gacctgcgct tcgctgtcca gctcggcctc gtccgcgatc
     2701 atttcgacca cgtcgatgcc gtcaagcgtc cagccgtgcg aatgcgcacc agcagccagc
     2761 tcttcccggg tttccgacag cgtgacgtaa aggcattttt cgcccatctt cgcgccgtcg
     2821 cgcagaaatt gcagtgacaa ggtggtcttg ccggccccgg gatcgccttc caccagatag
     2881 aggcgacggc gcggcagccc accgccgaga atatcgtcga gcccggcgat tcccgtgctg
     2941 caacgctcca gtcctttgct gtccgtcatg tgaatgggcc ctcccggcag gattctggtt
     3001 tctggctatt ttatagattt gttttgatca gctacggagc agccaaggtt gaagggacga
     3061 ggctcgttga ccccgtatca ttggcaaaag tttccggacg ttatcgggac agcgggacaa
     3121 ccaggaacca tggcgtcgtg gaccgcctgc gtaaaggccg ctcagttgct tttgcgcggc
     3181 cgattggtta ggttcgacgt ttacgcgggg tccttgcaat gagcggtttc gcagaatacg
     3241 aacaatacga tgcgttggga ctggcggaac tcgtacgccg caacgaagtg acccccggtg
     3301 aattgctcga cgcggccatc gagcgcgtgg aagcgcgcaa cccggccgtc aatgcagtca
     3361 cgatgaaact atacgactac ggccgaaagg cgattgccga tggtctgccc gatgggccgt
     3421 ttcgcggcgt tccgttccta atgaaagacc ttacatcgcc ggtagccggc gtgccgatga
     3481 cgcgcggttc aaaatatttt gccgatacac cgccttcagc catcgacagc gaacatgtaa
     3541 ggcgcctgaa aaaagcgggg ctggtgattt tcgggcgcac gaatacgtgc gaactcggcc
     3601 tttcgctgac gtgtgaaccg cagttgcacg ggccgacccg aaatccgtgg aaccttgccc
     3661 acatttccgg cggttccagc ggtggcgctg cggccgctgt ggccgctcgc atgctgccga
     3721 tcgcgcacgc gtcggatggc tttggctcga tccgcgcgcc ggctgcctgc tgtgggctgg
     3781 tcggactcaa gccgactcgc agccgcaaca ccttcgctcc gtttgcaggg gagggattgg
     3841 gtggcgtctc aaccgaacac gcagtgtcgc tcagcatacg cgacagcgcg gcgttgctgg
     3901 atgcaacgcg cggtgccggg ccgggagacc cctatgtcgc gccaccgccc tcgcgcccgt
     3961 atctgcagga agccggcgca gatccgggga aattaaaaat cgcatggacg agtgcagcgc
     4021 caaacggcgc gaccatcgat gcggcttgcc tgcgcgtgct tgcgcaaacc gtcaggcttt
     4081 gcgagcagct tggacatgac gtggaggaat gtgatccgaa aatagatcga gaggcagtcg
     4141 tgccgacatt cctcacgctg gccgctgcca acactgtcgt caatctttcc acccatccga
     4201 cagcgggtcg gccggcacgc gacagtgaag tggaacgcat tacgaggttg acggccaaaa
     4261 tgggggaaag cataagcgca gcggattacg tccgcgccac gcaaatggca catcggctgg
     4321 gacggcaaat ggcaacattc cataccgggt gggatgtatt gttgacaccg ggcctcagct
     4381 cggctgccgt caagctgggc tggctcgaca tgatgatgga cgacctgcag gaatactggc
     4441 gccgtgtgtt cgagttctcg ccgttcacag tctggttcaa cttaacgggt caaccggcac
     4501 tgatgttgcc gctcgatcaa acgctaggcg gtatgccgct tgcggtgcaa gcggtagcgc
     4561 gtttcgggga tgaggcgaca ttgattcgcc tgggtgcgca acttgaagcg gccaggccgt
     4621 ggttcgcccg caagccggca attgccgcgt gatgcaacgt tgatgcagtg aggcattcat
     4681 aatggacgca gaaaagccgg atctcaaagc gggcatttca ctggaccagc taccggatgg
     4741 tggctt
//
LOCUS       DATMZQ010000366        13387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_290654, whole genome shotgun sequence.
ACCESSION   DATMZQ010000366 DATMZQ010000000
VERSION     DATMZQ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290654"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VN496_11795"
     CDS             <1..116
                     /locus_tag="VN496_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXF17683.1"
                     /translation="AGGVWSLPPTLRFGDQDRDKYLAIGESDRMTLKFVKP"
     gene            120..1379
                     /locus_tag="VN496_11800"
     CDS             120..1379
                     /locus_tag="VN496_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXF17684.1"
                     /translation="MNDSLRLIVQVDAAAAALEAKVIGWRRDFHQHPELSNRETRTAG
                     VVTDHLRRLGLKVETGVAHTGVVGLIDSGKPGPVIALRADMDALPVTEQVDVPFASKA
                     RATYNGQDVGVMHACGHDCHVAILMGVAEVLAAAKDSLKGKVKFIFQPAEEGPPAGEE
                     GGAALMIKQGVLENPKPDVIFGLHVFAGIEAGRIAYRPGPTMASADRIRVVVNGRQTH
                     AALPWRGVDPIVISSQIVLGLQTIISRQVDVSLEPAVLSIGAIRGGIRDNIIPDTVEM
                     LGTVRTFDEAMRADIRARIRNTVEMIAKSAGGSAHVEFDSAYPVTVNDVPLTERMVPT
                     LERVAGKSNVFLGQKITGYEDFSHYQQRIPGFFYFVGITPSGIDRKQSAPNHSPHFFV
                     DESALLVGLRSLVHLTLDYMASTTQPG"
     gene            1416..1904
                     /locus_tag="VN496_11805"
     CDS             1416..1904
                     /locus_tag="VN496_11805"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF17685.1"
                     /translation="MTRNRKLISFLLFLAAVFIPATLLRADTDEKGFIRVLPENIEWK
                     DAPGYNGVKMAVIAGDPSKPGVYVVRVKFPPGLMSRPHFHPDDRYAVVLKGTWWTGTG
                     DTFDPASTVPVPAGGFMKHPAGGHHFDGAKDEEVILQLIGYGPGGTTMIHPEEGHTGP
                     SR"
     gene            1985..3661
                     /locus_tag="VN496_11810"
     CDS             1985..3661
                     /locus_tag="VN496_11810"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXF17686.1"
                     /translation="MLSDIEIAQNARMQRISVLARERLGIAEENLEPYGHFKAKLSLE
                     YLDTLRDRADGKLILVTAISPTPAGEGKTTTTVGLGDALNRIGKKALIALREPSLGPV
                     FGMKGGAAGGGYAQVVPMEDINLHFTGDFNAIQLANNLLAALLDNHVHHGNELGIDVR
                     RVSWKRVLDMNDRALRDIVVSLGGPGNGYPRQDGFDIVVASEVMAIFCLATSLADLKQ
                     RLGNIVVAYTREQSPVRARDLAAHGAMTVLLKDALKPNLVQTLENTPAILHGGPFANI
                     AHGCNSVIATRAGLKLADYVVTEAGFGADLGAEKFVDIKCRKSGLRPDACVIVATLRA
                     LKYHGGADLKDLNRENLAALEKGLANLERHISNVRTQFGLPCVVSINQFTHDTDAEIA
                     LLKHRISTPDTPVVLAQHWARGGAGAEELARAVVRTIDKGVSGFKYVYEDSATLWDKV
                     RAVATKIYGAADVTADAKVKAQIQKLQEDGYGHYPICIAKTQYSFSTDASARGAPSGH
                     IINVREVRLAAGAEFVVMVCGDIMTMPGLPRVPAATKIDLTADGKVVGLF"
     gene            3715..5499
                     /locus_tag="VN496_11815"
     CDS             3715..5499
                     /locus_tag="VN496_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF17687.1"
                     /translation="MRSEFKATTALSVQRGETSAVQDLDWLAQNIPCQAACPAGTDIP
                     GYLEAIYHGRFADAYAINLRDNVFPAVLGRVCSRPCEHACRHGWEGNGEPVAICFSKR
                     SAADFGNSAPVHLQPSAATGKTVAIVGSGVAGLAAARDLALAGHRITVFEKHSAPGGM
                     LNQGIPAFRLPRTVIAREIQQVIATGVEIRCRVEVGKDISLAELSARFDAVIMAAGTL
                     RPNILELPGKELQGIEHGLSFLLHANEFGRRDIGARVIVIGGGYTAMDCARTARRLGA
                     EVSVVYRRSRDDMVVLPGEVEELIEEGGALLPNYSPVAFLGETGKAASVRFMATRNGA
                     PGRDGRHLPIDVPGSELSLRADTVIMATGQFPLTEWIREPLKGKLVGQDGWLASGTTH
                     ETPEENIFAAGDFALGATTLIQAIGHARECARKVDRWLTGAAPLRARAKLGPIFQSKV
                     PGGHDTNRTPQMNVIPIHVMPTLPVSARTLSVEVETGFSPATASEEASRCYLCHYKFE
                     IIDEKCVLCDECLKVKPVEGCIVEIASLLRDGEGRITGYTRIEQGKTDSLYYNRLWID
                     QSQCVRCGRCEAVCPVNAITIQKVSLHA"
     gene            5712..6251
                     /locus_tag="VN496_11820"
     CDS             5712..6251
                     /locus_tag="VN496_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXF17688.1"
                     /translation="MAGVPSRWLMVEEAISYYARRMVAWSLGDPVATYRGGICRLTGL
                     RSELSTHSIIAIKGAVDFVKDHHTEPHLSNTSLFFRDRHMCAYCGGQYKERDLSRDHV
                     IPVYLNGRDRWMNVVTACRSCNIRKGGRTPEEAHMPLLYTPYVPNRYEHLILQNRRIL
                     QDQMEYLMAKVPRYSRLYV"
     gene            6379..7218
                     /locus_tag="VN496_11825"
     CDS             6379..7218
                     /locus_tag="VN496_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXF17689.1"
                     /translation="MQRFVLTAILAILALTGCQSNPVTGRKQLMLVSEDTAINESKQA
                     YVAMLQPLAQQHKIDTNPATVARVHEITGRLIAQAVKYRPETEKWDWSVKVIDDPKTV
                     NAWCMAGGKMAIYTGLIQQIQPTDDELAQVMGHEISHALAKHTQERMSRAMAMQVGLG
                     AVALSQQNSKYGNLALTGAQAAAVVGLELPNSRTAESEADRIGIELAAKAGYDPHAAV
                     TLWQKMAKVGGGDPNSRNDFLSTHPAPVKRMETLAELVPQMMPYYADKSPRPMYQLHA
                     SNP"
     gene            7577..8953
                     /locus_tag="VN496_11830"
     CDS             7577..8953
                     /locus_tag="VN496_11830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXF17690.1"
                     /translation="MNSIRGYFTGRAISSSSASVSSWRNLLRLSAIGFSVIAALQCGT
                     CFAASAQKDPALRALAEAIEQVRAKYVGVVDEKKMVSDAIRGIVQGLDPYSDYLDPSS
                     YSELRQDNSGRFGGLGLEVAMDGGAARVVATIENSPASRAGLQPGDLVTGIDGVSLEG
                     FTLDQTIRRARGEPDTSVALTVLRKADAQASVITLKRAIIQSRSVTSALLGSGYGYIR
                     ISQFTQRTAESTVTELAQLYRKGGGNLKGLLIDLRDNPGGLLRSAVAVSSIFLPKDSL
                     VVYTEAAHAESRMRLKSSEDQYQPKGRDEVLKQMPDLKTLPLVVLVNSGSASASEIMA
                     GALQDNRRATVVGTQTFGKGSVQVLVPLEDGAALKLTTAYYFTPNGKRIQGKGVTPDT
                     LLDQLAIDKVAGIKPAVHGTTPADTISLACGYSAADVPVADAVVGSDCQLLRAIELLR
                     LLPVLTHS"
     gene            complement(9059..13000)
                     /locus_tag="VN496_11835"
     CDS             complement(9059..13000)
                     /locus_tag="VN496_11835"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXF17691.1"
                     /translation="MTLLAIPRALRRIAIRMLWLFATLTLIIVAGFLYVTFVGISVDA
                     SFLRNTIAQTFSDNIGRSVRFEGPMEMEISAKPKLRVGGLHVANPPGFGDEDFASLGE
                     ARLALDLWPLLFKRRLHIEELAGSDVHARLQVHRDGSNNWTFRRPLPEAAKSPQPAAT
                     SSMRTDQALALLDIRKISLEKLRVRYTGADNVQHFFSLHALKAHSPSDEAFTLSLNGA
                     IEKEFPYRLEFTGGKLADLATSKPWPVSFTLSFLSSTLAVNGSFSGARGEFIFGLGTE
                     NLLEFQRLFQVKLPDVGASGIAGSVVFDPNRISLTNLAGAMGKTTLVGALDVDNAGPK
                     PKVSGSLVLPTLDLRPFLGDKSPAPSAEPPHSLADVYRNLSTATFNLRQMNNVDLDLR
                     LGVGRWLSLPGEVKDVTLQLKLADGMLQAPLNASVAGVVLSGNVEADATADPPQFKIA
                     LGTRDSELGGLAELLAGVRGVKGHLGRFDVRLAANGDQGNELVRSLDVALDLNNGSLS
                     YGNSAGERPVAFTLGKFAVRLPAGKELTGDMRGTLLNHPFSAQLRGGVLESMMLNARA
                     PLDFRLHSTDVRARINGVLQLPEPSSGPELAFEFSAPHAGALASWFGFKPGAEAPASL
                     SGKASLRTSRWQVRDLLFSLGRTTLSADLSQEVVAGKPLVKIRLNADQVDVKELEALI
                     PQAEQKKDQPERPVLDIPLLPQGIDLTDADVNVNVKRLAGTAVEVRDISFDGRVREGY
                     MYPSPFAVKVDDTGFNGAVLIDLRSAEPTAGLWLYAGNLDVGKLLRKLGVVQNLDAIF
                     SEFGVNLVARSSRLGDMLARSELLGSIGGGRIVLRDPNTHAEARITLDKGELRADPGA
                     QLKLTLKGGLDDVPVSIALETARAYELADPKLPLPFKLDVEAADTGVTLSGNVARPVG
                     SELELALDASGQRFDSLNKLARISLPPWGPWSASGKFRMSPRGYEVNGMRLQVGASVL
                     EGSGRVETQSGRPGIDVVLRAPVVQLDDFKLGDWSPIEKKPDDKTVAPTAEAVRSKTA
                     AASDQAQKLLSRETLRRQDVRLKVEVNQVLSGNDKLGSGKLEAKLENGRADIGPVVVD
                     VPGGGAKLWLGYEPTDQDVKVDMHVDVQKFDYGVLARRIKPESDLHGTFSLKMDVDSR
                     ALYLSDTLRHGSGRIEFAVWPQNMHSGIFDLWAVNVLVALVPAVDPGKASKVNCAIGR
                     FDLKDGKLVERSIILDTSRMRVNGTGTANFSDEAFKVRMSPQAKTAQFLSLETPIEVG
                     GTFSNFKIGVSPGDIVGTVGRLATSILWVPLQKLAGKKIPTDGADVCYPSF"
     gene            complement(13106..>13387)
                     /locus_tag="VN496_11840"
     CDS             complement(13106..>13387)
                     /locus_tag="VN496_11840"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HXF17692.1"
                     /translation="PVATLLGWNTRTRDFGAPDLCDLLGSTIPLPASRIDGKSRNDPR
                     PSLEELYGTHDSYVRKIADAAKKLQAARLMLPEDVDMIVQEAENSDVLR"
BASE COUNT         2785 a         3913 c         3962 g         2727 t
ORIGIN      
        1 atgcgggcgg cgtgtggagt ttgccgccga cgcttcggtt tggcgaccag gaccgcgata
       61 aataccttgc gatcggagaa tccgatcgca tgacccttaa atttgtaaag ccctgaccca
      121 tgaatgattc attgcgttta atcgtgcaag ttgatgccgc tgctgctgcg cttgaagcca
      181 aagtaatcgg ctggcgccgc gattttcatc agcatcccga actgtcgaat cgggaaacgc
      241 gcaccgccgg ggtcgtgacc gaccatttgc gccggctcgg gctcaaggtt gagaccgggg
      301 ttgcgcatac cggcgtggtc ggcttgatcg actcaggcaa gccgggtccg gtgatcgcgc
      361 tgcgcgcgga catggatgcc ctgcccgtga ctgagcaggt tgatgtgccg ttcgcgtcga
      421 aagcgcgggc gacctacaac ggacaggacg tcggcgtcat gcatgcctgc ggccatgatt
      481 gccatgttgc gattctcatg ggagtagccg aagttctggc cgcagcaaag gacagcctta
      541 aaggcaaggt caaatttatt ttccagccgg ctgaagaagg cccgccggct ggcgaagaag
      601 gtggcgccgc gctgatgatc aagcagggcg tgctggagaa tcccaagccc gatgtcattt
      661 tcggtctgca cgtgtttgcc ggcatcgagg cgggacgcat tgcgtatcga cctggcccca
      721 cgatggcgag cgcggacagg atacgcgtgg tcgtcaacgg ccggcagacg catgcggcat
      781 tgccatggcg cggcgtggac ccgattgtga tctcctcgca gatcgtgctg ggcttgcaga
      841 caatcatcag caggcaggtg gatgtatcgc tagagcctgc cgtgctgagc atcggcgcca
      901 ttcgcggcgg catacgggac aacattattc cggacacagt ggaaatgctc ggcacggtaa
      961 ggacttttga tgaagcgatg cgcgctgata tccgcgcgcg catacgcaat actgtggaga
     1021 tgatcgccaa aagcgccgga ggatcggcgc acgtcgaatt cgatagtgcc tacccggtca
     1081 ccgtgaacga cgtgccgctg accgagcgca tggtgccgac gctggagcgg gtggcaggca
     1141 agagcaatgt gttcctgggc cagaagatca caggctacga ggatttttcg cattatcagc
     1201 aaagaatccc gggcttcttc tactttgtcg gcattacgcc cagcgggatt gatcgcaagc
     1261 aatcggcgcc caaccattca ccgcatttct tcgtcgatga atctgcattg ctggttggct
     1321 tgagatctct cgtgcatcta acgctcgatt acatggcatc gactacccag ccgggttaaa
     1381 atccgcgcca acgaccatac gagccggagg atattgtgac ccgtaaccgc aagctgattt
     1441 cattcctgct gtttctggcg gcagtcttca tacctgcgac gctgctgcgt gcggacaccg
     1501 atgaaaaggg ttttatccgg gttctgccgg aaaatatcga atggaaagat gcacccgggt
     1561 acaacggggt caagatggct gtgattgcgg gagatccgtc caagcctggc gtgtacgttg
     1621 tccgtgtgaa gtttccgccg gggctgatgt cgcgtccgca tttccatccc gatgatcgtt
     1681 atgccgtggt actcaaggga acctggtgga caggcaccgg ggacactttc gatcccgcct
     1741 cgactgttcc ggtaccggcc ggcggcttca tgaagcaccc ggccggcggt catcatttcg
     1801 acggcgccaa ggatgaggaa gtcattctgc agttgatcgg ctatggcccg ggaggcacga
     1861 ccatgattca cccggaggag ggtcataccg gcccatccag ataatttctg gcaatgctca
     1921 ccaagcggca gagctctctg ccgctttttc gtttccaaac gcatggataa acacaaggcg
     1981 caccgtgctt tccgatattg aaatagccca gaacgcccgg atgcaacgca tcagcgtgct
     2041 ggcgcgcgag cgtcttggca tcgcggaaga aaatctcgaa ccgtacggcc acttcaaagc
     2101 caagctttca ctggaatatc tcgacaccct gagggatcgc gctgatggca agctgatcct
     2161 ggtaacggcg atcagtccaa caccggccgg cgaaggcaag actacgacca cggtcgggct
     2221 gggcgacgcg ttgaatcgca tcggcaaaaa ggctttgatt gcgctgcgcg aacccagtct
     2281 tgggccggta ttcggcatga aaggcggcgc cgcgggcggt gggtacgcgc aagtggtacc
     2341 catggaagat atcaatcttc atttcacggg cgacttcaac gcgattcagc ttgccaataa
     2401 tctgctggcg gcactgctgg acaatcatgt ccatcatggc aacgaactcg gcatcgacgt
     2461 gcgtcgggtt agctggaagc gtgtgctgga catgaatgat cgtgccttga gggacatcgt
     2521 tgtgtccctc ggcgggccgg gcaatggtta ccccaggcag gatggcttcg acatcgttgt
     2581 ggcatccgaa gtcatggcga tcttctgcct ggcgacttcg ttggccgatc tcaaacaacg
     2641 actcggcaat atcgttgtcg cgtacaccag ggagcaatcg ccggtccgtg cgcgcgacct
     2701 tgcggcgcat ggcgccatga ccgtgttgct caaagatgcg ctcaagccca acctggtgca
     2761 aacgctggaa aatacgccgg ccattctgca cggcgggcca ttcgccaaca ttgctcacgg
     2821 ctgcaattca gtcattgcca cacgagccgg cctcaaactg gcggactatg tcgtgacgga
     2881 ggccggtttc ggtgccgatc tcggcgcgga aaaatttgtc gatatcaaat gccgcaaatc
     2941 gggacttcgc cccgacgcct gcgtcatcgt tgccactttg cgcgcgctga aatatcacgg
     3001 cggtgcagat ctcaaggact tgaaccgcga gaacctggct gctttggaga agggcctggc
     3061 gaacctggag cgtcacatca gcaacgtgcg gactcagttc gggctgccct gcgtggtatc
     3121 gattaaccaa tttacgcacg acaccgacgc cgaaatcgca cttttgaagc acaggatttc
     3181 cacgccggac acgccggtcg tcctggcaca gcactgggcc cggggcggcg ccggagcgga
     3241 agaactcgcg cgtgcagtcg tccggaccat cgacaaaggt gtttccggat tcaaatatgt
     3301 ctacgaggat tctgcgacgt tgtgggacaa ggtgcgcgca gttgctacaa aaatttacgg
     3361 cgcggcggat gtgactgcgg acgcaaaggt caaggcgcag atccagaagt tgcaggagga
     3421 cgggtatggg cactatccaa tctgcatcgc caaaacgcag tattcgtttt ccaccgatgc
     3481 atcagcgcgc ggagcgccgt ccgggcacat catcaatgtg cgcgaagtac gccttgcggc
     3541 cggtgcggag tttgttgtga tggtctgcgg tgacatcatg accatgcctg ggttgcccag
     3601 ggtgccggcc gcgacgaaga tcgatctgac agcggatggc aaggtagtcg gactgttcta
     3661 gcctgaaaat gatgagtatc gtccaggcag caagccagcg cgatagtcgc cgcaatgcgc
     3721 tctgaattca aagccacaac ggcgctatcc gtccagcgtg gcgaaacatc ggccgtgcaa
     3781 gatctggact ggctcgcgca gaatatcccc tgccaggcag cgtgtcccgc cggtaccgat
     3841 attccgggct acctcgaagc gatctaccat ggacgctttg ccgacgccta cgcgatcaat
     3901 ttacgagaca acgtatttcc cgccgtgctg ggcagggtgt gttcacggcc ttgcgaacat
     3961 gcatgccggc atgggtggga aggcaatggc gaaccggtag ccatttgctt ttcgaagcga
     4021 tcagctgctg attttggcaa cagcgcgcca gtgcatttgc aacccagtgc tgccactgga
     4081 aaaactgttg ccattgtcgg ttcgggtgtc gctggccttg cagcggcgcg cgatcttgcg
     4141 ctcgccggcc atcggattac cgtgttcgag aaacacagtg cgccaggagg catgctgaac
     4201 cagggcatcc cagcgtttcg cctgccaaga accgttatcg cccgtgaaat ccagcaagtg
     4261 attgccaccg gcgttgagat ccgctgccgc gtagaggtag gcaaggacat ttcgcttgcc
     4321 gaactgtcag cgcgatttga cgccgtcatc atggcggcgg gtactttgcg cccaaatatc
     4381 ctggagctgc caggcaagga attgcaaggc atcgaacacg gcctttcgtt tctgctgcat
     4441 gccaacgaat ttggccggcg cgatatcggc gctcgggtca ttgtgatcgg cggcggctac
     4501 acggccatgg attgcgcgcg cacggcgcgg cgcctgggtg cggaggtcag cgtggtctat
     4561 cggcgttcgc gcgacgacat ggttgtgctg ccaggggaag tggaagaatt aatcgaagag
     4621 ggcggtgctc tgctaccgaa ctattcgccg gtagcgtttc tcggtgaaac tggcaaagcg
     4681 gccagcgtgc gcttcatggc tacccgtaat ggagcgcccg gccgggatgg caggcacttg
     4741 ccgatcgatg ttccaggctc ggaactgtcc ttgcgtgccg ataccgtaat tatggccacc
     4801 ggtcagtttc cattgaccga gtggatccgc gagccgctga aaggcaaatt ggttggacag
     4861 gacggctggc tggcgagcgg caccacgcac gagacgccgg aagaaaacat ctttgcagca
     4921 ggcgattttg cgctgggtgc gacgactctg atccaggcca tcggacatgc gcgggaatgt
     4981 gcccgcaaag tagatcgctg gctaactggc gctgcgcctt tgcgcgcacg tgccaaactc
     5041 ggcccgatat ttcagtcgaa ggtccccggt ggccatgaca ccaatcggac cccgcaaatg
     5101 aatgtcattc caatccatgt aatgccgaca ctgccggtct ccgcgcggac gctgagcgtt
     5161 gaagtggaaa ccgggttttc ccctgcaacg gcaagcgaag aagcgtcccg ctgttacctg
     5221 tgtcactaca agtttgaaat catcgacgag aaatgcgtcc tttgtgacga atgcctcaag
     5281 gtgaagccag tggagggctg catcgtggag atcgcttcgc tgctgcgcga tggcgaagga
     5341 cggatcaccg gctatacccg catcgaacag ggcaagacgg actcgctgta ttacaaccgg
     5401 ctctggattg accaaagcca atgcgtccgc tgcgggcgct gtgaagcagt ctgtcccgtc
     5461 aatgccatta ccatccagaa ggtctcgctg cacgcctgac gtacccggca gaccggcgcg
     5521 caaccggcct tgccgtcccg gctggcttcc aaaatcgcat ttcttgcggc tatactgcgt
     5581 ttgaaggccg tgtggcagcc ccaatatgcg gttccgggcg accgccggtg aagagccgaa
     5641 gaccgttggt tgtccgcgct tcggcatgac ttgtcaggag agcggagttg gccctggttc
     5701 ttgcggtcga tatggctggc gttcccagcc ggtggttgat ggtcgaggaa gccatttcgt
     5761 actatgcgcg gcgcatggtc gcatggtcgc ttggcgatcc ggtcgcgacc taccgcgggg
     5821 gcatttgccg ccttaccggc ctgcgctccg aactgtccac acacagcatc attgcgatca
     5881 aaggcgcggt cgattttgtc aaagaccacc acaccgaacc ccatctgtcg aatacgtcac
     5941 tctttttccg cgatcggcac atgtgtgcgt attgcggggg tcaatacaag gaacgcgatc
     6001 tgtccagaga tcacgtgatt ccggtttacc tgaatggacg cgatcgctgg atgaacgtcg
     6061 tgaccgcgtg ccgctcctgc aatatccgca agggcgggcg cacgcctgaa gaagcacaca
     6121 tgccgctgct gtatacgcct tacgtgccca atcgctacga acatttgatc ctgcaaaatc
     6181 gccggattct gcaggaccag atggaatatc tgatggcgaa ggtgccgagg tacagccgct
     6241 tgtacgtctg attcgctcaa atgcactgaa aagccggctc cagccggctt tttcttttgt
     6301 gcaatgcaca tgccaggcgg attatgctgc tgaacaaaag ccgggttgcc gaatccaacg
     6361 aagcacgagg agaaaaccat gcagcgattt gttcttaccg ccattcttgc gattctggca
     6421 ctcacgggtt gccagtccaa tccggttacg ggccgcaaac aattaatgtt ggtctccgaa
     6481 gacaccgcga tcaacgagtc caaacaagcc tatgtcgcca tgctgcaacc gctggcccag
     6541 cagcacaaga tcgacaccaa tcccgccaca gttgcgcgag tgcacgaaat caccggccgg
     6601 ctgattgcac aggcagtgaa ataccggccg gaaaccgaga aatgggattg gagcgtcaag
     6661 gtaatcgatg acccgaagac cgtgaatgcc tggtgcatgg ctggaggcaa gatggccatc
     6721 tacacgggac tcatccagca aatccagccg accgacgatg aactggcaca ggtcatgggt
     6781 catgaaattt cacatgcact ggcgaagcac acgcaggaac gaatgtcacg tgccatggcc
     6841 atgcaagtag ggctgggcgc agtggccctg tcacagcaga acagcaagta cgggaatctg
     6901 gcgctcactg gtgcccaggc tgccgcggtc gtcgggttgg aactgccaaa cagccgcact
     6961 gcagagtcgg aagccgatcg catcggaata gaactcgccg ccaaggcggg ttatgacccg
     7021 cacgccgccg ttaccctgtg gcaaaaaatg gctaaggtgg gcggcggcga tccaaattcc
     7081 cgcaatgact ttctgtccac gcatcccgcg cccgtaaagc gcatggaaac cctggctgaa
     7141 ctggtcccgc agatgatgcc ttactatgct gacaaatcac ctcgtccgat gtatcagttg
     7201 catgcatcca acccttgaaa ccagggctca ttgatcggat tgcgcgcatc atcagatagc
     7261 gcctgacgag cgtctcaatt tttaacgcct gcaaatcacg ggcggcaata acaggcgcgc
     7321 tgagtcggcc gcgcgatttg cacggcatcg atcgcactca ttcgagcgcg cgacaattac
     7381 cgcaggcacc gggcgctaga aaaaatttcg cgctttcaaa ttcgcagcgc ccgcgaaaaa
     7441 ttcgacggta cagtctcttg taattcttgc aacttgcatc ctgtcgccgc agtgagacgg
     7501 gtacggaaaa atcagtagag ctttcgctgc attcccgtaa gatacggcct acaaaacatg
     7561 cgggagacaa aacaagttga attccatacg ggggtatttc accgggcgcg caatcagcag
     7621 cagcagcgca tcggtctcca gctggcgcaa cttactgcgc ctttcggcga tcggtttttc
     7681 ggtcattgca gcgcttcagt gcggcacctg ttttgccgca tctgcgcaaa aagatccagc
     7741 gcttcgcgca ctcgccgagg ccatcgagca agtgagggca aagtatgtcg gtgttgtcga
     7801 cgagaaaaaa atggtgtcgg acgccattcg cggcattgtc cagggtctgg atccttattc
     7861 ggattatctg gatccatcgt cctacagtga gctgcgccag gataacagcg gaaggtttgg
     7921 cggcctaggt ctcgaagtcg cgatggatgg cggtgcggcg cgcgttgttg ctaccatcga
     7981 gaactcgccg gcgtctcgcg cgggattgca gcccggagat cttgttaccg ggatagacgg
     8041 cgtaagcctc gagggtttta cgctggacca gaccatccgc cgcgcacgcg gcgaaccgga
     8101 caccagcgtt gcgcttaccg tgctacgcaa ggcagacgcg caggccagcg ttattacgct
     8161 caagcgtgcg atcatccaaa gtcgcagcgt gacttccgcg ctactgggct cgggttacgg
     8221 gtatatccgg atcagtcaat tcacgcagcg tactgccgaa tcgaccgtta cagagctggc
     8281 gcagctctat cgcaagggag gcggcaatct gaaaggattg ctcatcgacc tgcgcgacaa
     8341 tcccggcggc ctcctgcgat ccgcagtggc cgtatcctcc atattcctgc cgaaggattc
     8401 tctggtcgtg tacaccgagg ctgcgcacgc ggagtcccgc atgcgtttga aatccagtga
     8461 agatcaatat cagccaaaag gccgggatga agtgttgaag cagatgcccg atctaaaaac
     8521 gctgcctttg gtggtgctgg tcaattccgg gtcagcgtcc gcatctgaaa tcatggcggg
     8581 cgccttgcag gacaatcgtc gggccacagt cgtgggcacg cagactttcg gcaaagggtc
     8641 ggtccaggtt ctcgtgccac ttgaagatgg cgccgcattg aagctcacca cggcatacta
     8701 cttcacgccc aatggcaagc gaatccaggg caagggcgta acgccagaca cgctgctgga
     8761 tcaactggcg atcgacaaag tggccggaat caaacccgca gtgcatggaa ccacgcccgc
     8821 ggacacgatc agtcttgctt gcgggtattc tgcggcagat gtaccggttg ccgatgcagt
     8881 ggtcggcagc gactgccagt tgctgcgggc gattgaacta ctgcgtctgc tcccggtact
     8941 cacgcacagt tgagctgttc ctgaattgac gcaaggatgg acgaagcagg gcgcagcaga
     9001 tgcgcccttt ttcttgtgta ttccctcaat gcgagttcgg ggaagctgtc gtcgagggct
     9061 aaaacgacgg gtagcagacg tcagcgccgt cggtggggat ctttttgccg gcaagctttt
     9121 gcagcggcac ccagagtatg gacgtcgcga ggcggccaac cgttcccacg atgtcgcctg
     9181 gggacacacc gattttgaaa ttactgaacg tcccacccac ctcgattggc gtttccaaac
     9241 tcagaaactg cgctgttttg gcctgcgggc tcatacgaac tttgaaagct tcgtccgaaa
     9301 agttagctgt gccggtcccg ttgacgcgca ttctgctggt atccagaatg atgctccgct
     9361 caaccagctt gccgtccttc aagtcaaagc gcccgatggc gcaattcacc ttggatgctt
     9421 tgccaggatc gactgcaggc accaaagcca ccagaacatt gaccgcccac aagtcgaaaa
     9481 ttccggaatg catgttttgc ggccagacgg cgaattcgat gcgtccgcta ccatggcgca
     9541 gcgtatccga cagataaagt gcccgggaat cgacgtccat cttcagactg aatgtgccgt
     9601 gcagatcgct ctcgggcttg atgcggcgcg cgagtacgcc atagtcgaac ttttgtacat
     9661 cgacgtgcat atcaaccttc acatcctggt cggtaggttc atatcccagc cacagtttgg
     9721 cgccaccgcc gggcacatcg accaccaccg gtccgatgtc cgcgcgcccg ttctccagct
     9781 tggcttccag tttgccgcta cccagcttgt cgttgccaga cagcacctga ttgacttcga
     9841 ctttaaggcg cacatcctgc cggcgcagcg tttcgcggct gagtaatttc tgcgcctgat
     9901 cactcgccgc ggcggtcttg ctgcggactg cttcggcagt gggggccacg gtcttgtcat
     9961 cgggtttctt ttctatcgga gaccaatcgc cgagtttgaa gtcgtccaac tggacgaccg
    10021 gagccctcag cacgacatcg atgccgggac gcccgctctg tgtctccaca cgtccactgc
    10081 cctcgagaac gcttgcgccg acttgcagtc gcataccgtt gacttcatag ccgcgtggcg
    10141 acatgcgaaa cttgccagat gccgaccagg gcccccaggg tggcagggat atgcgcgcaa
    10201 gtttattcag gctatcgaaa cgctggccac tcgcatccag cgccagctcg agctcgctgc
    10261 ccacgggccg ggcaacgttg ccggacaggg tcacgcccgt atccgccgct tcgacgtcca
    10321 gtttgaacgg cagcggtagt tttggatccg caagctcata ggcccgggca gtttccagcg
    10381 caatcgacac gggcacgtcg tcgagtccgc ctttcagggt caacttgagt tgagcgcccg
    10441 ggtcggcgcg caattcccct ttgtcgagcg tgattctggc ctctgcgtgc gtgttcgggt
    10501 cgcgcagcac gatgcggcct ccaccgatcg aacccagcaa ctcggagcgt gccagcatgt
    10561 ctccgagccg gctggaacgt gcgaccaggt tcacgccgaa ttcgctgaat atggcgtcca
    10621 gattttgcac cacgccgagt ttgcgcagaa gcttgccaac atcgaggttc cctgcataaa
    10681 gccataagcc tgccgtcggc tctgcgcttc gcaggtcgat cagtaccgcg ccattgaagc
    10741 cggtatcgtc aactttgaca gcaaacgggg acgggtacat atagccctcg cggacgcgac
    10801 catcgaaaga aatgtcgcgc acttccaccg ccgtgccggc aaggcgcttc acgttcacat
    10861 tcacgtccgc atcagtcaga tcgataccct gcggcaacag cgggatgtcc agaaccggac
    10921 gttccggctg gtccttcttt tgttccgctt gcggaataag cgcctcgagt tccttgacat
    10981 cgacctggtc cgcgtttagt cgtattttga ccagcggttt gcctgccacg acctcctgcg
    11041 agagatctgc gctcagcgtc gtccggccaa ggctgaaaag caggtcgcgc acctgccagc
    11101 gcgatgtgcg caagcttgct ttcccggaca gtgaagccgg tgcttcggcg ccgggcttga
    11161 aaccgaacca acttgccaac gctccggcat gcggcgctga aaactcgaaa gccaattccg
    11221 gcccgctgct cggctccgga agctgcagta cgccgttaat gcgtgcgcgt acgtcggtgg
    11281 agtgcaacct gaagtccagc ggcgcgcgcg cgttcagcat cattgactcc agtaccccgc
    11341 cgcgtaattg cgcgctgaac ggatggttca aaagcgttcc gcgcatatcg cctgtcagtt
    11401 ccttgccggc cggcaggcga acagcgaact tgccaagggt gaacgccacg ggtcgttcgc
    11461 ctgcgctgtt gccgtagctg aggctcccgt tgttgagatc cagcgcgaca tcgaggctgc
    11521 gcaccagttc gttgccctga tcgccattgg cagccaaccg gacatcaaag cgtccgagat
    11581 gtcctttgac gccacgcacg ccagccaaca attcggccaa tccgccaagc tccgaatcgc
    11641 gtgtgcccag cgcaattttg aattgtggtg gatcggcagt cgcgtccgct tcaacattgc
    11701 cagacaacac gacgccagcc actgatgcat tcaggggtgc ctgcaacatc ccgtccgcga
    11761 gtttcaactg caacgtgaca tcttttacct cgcctggcag gcttagccat cggcctacgc
    11821 cgagcctcag gtcaagatcc acgttattca tttgcctgag gttgaacgtc gcggtcgaca
    11881 gattgcgata aacatccgcc aggctgtgcg gcggctcagc gctcggcgcc ggcgacttgt
    11941 ctccgaggaa cgggcgcagg tccagagtcg gtagtaccag cgaacctgac actttcggtt
    12001 tgggcccggc gttgtcgaca tccagggcgc cgaccagcgt cgtcttgccc atcgcgccgg
    12061 ccaggttcgt caaactaatc cggttcggat cgaaaacaac tgacccggcg ataccgctcg
    12121 cgccaacgtc tggcagcttg acctgaaaca gacgctggaa ctcgagcaga ttctcggttc
    12181 cgagaccgaa aataaattca ccgcgcgcgc cactaaagct gccgttcacc gccagcgttg
    12241 aactgaggaa acttagcgtg aaggaaacgg gccatggttt gctggttgcc aggtcggcga
    12301 gcttgccacc ggtgaactca agccgatatg gaaactcctt ctcgattgcg ccgttcaggc
    12361 tcagcgtaaa cgcttcgtca gaaggcgagt gcgctttgag cgcatgcaga ctgaagaagt
    12421 gttgcacatt gtctgcgccg gtgtagcgca ctcgcaattt ttccagcgat attttgcgta
    12481 tgtcgagcag cgcaagagcc tggtccgtgc gcatggatga ggtcgcggcc ggctgcgggg
    12541 atttggccgc ctcgggtagc gggcgccgga aagtccagtt gttgctgccg tcgcggtgga
    12601 cctgcaaacg ggcatgcaca tcactgccgg ccagttcctc gatatgcagc cgtctcttga
    12661 acagcagcgg ccaaaggtcg agtgccagac gcgcttcgcc caggctcgca aaatcctcgt
    12721 caccgaatcc cggagggttg gcgacgtgca ggccaccgac gcgcaatttc ggtttcgccg
    12781 aaatttccat ttccattggg ccttcgaacc gcacgctgcg gccgatgttg tcgctgaacg
    12841 tctgtgcaat ggtgttgcgg aggaatgacg catccaccga aatgccgaca aaagtgacat
    12901 agagaaaacc ggccacgatg atcagggtga gcgtggcgaa tagccacagc atgcggatcg
    12961 caatacggcg cagcgcacgt ggaatggcca gcagcgtcat gatttactcg atgacgcagt
    13021 cttcggaccc gcacgcgttc tggctcgcat tctgcgggtg gcccttgaag cgtacacacc
    13081 tgggcacggg cggctacccg ggcaactaac gcaacacatc cgagttttcg gcctcctgca
    13141 cgatcatgtc cacgtcttca ggcagcatca agcgcgcggc ttgcagcttt tttgcggcat
    13201 cggcgatctt gcgcacatag ctatcgtgag ttccatacaa ttcttccagg gacggccgtg
    13261 gatcattcct gctttttccg tcgatgcggg atgcaggcaa tggtatggtc gatccgagca
    13321 gatcgcacag gtcgggtgca ccgaaatccc tggtgcgcgt gttccaaccc agcagcgtgg
    13381 caaccgg
//
LOCUS       DATMZQ010000367         5149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3053784, whole genome shotgun sequence.
ACCESSION   DATMZQ010000367 DATMZQ010000000
VERSION     DATMZQ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053784"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VN496_11845"
     CDS             <1..961
                     /locus_tag="VN496_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HXF17693.1"
                     /translation="PYVLIEGMAICGIAVGATRGYIYVRSEYPHACNILDDAITLARD
                     QHYLGDNILGSDKSFELEVRKGAGAYICGEETSLLESLEGKRGMVRFKPPLPAISGLF
                     GKPTVINNVITLASVPIIFAKGADHYRNYGLGRSRGTLPIQLAGNLKHCGLVEKAFGV
                     TLRELLYDYGGGSASGRPLRAVQVGGPLGAYLPEQQFDTPLDYEAFAAIGAMVGHGGI
                     VAFDDTVDMASMARYAMEFCAIESCGKCTPCRIGSTRGVEVIDHIVAGDEREKHIALL
                     EDLCDTMINGSLCALGGMTPFPVQSALKYFPNDFHVAPKRKVA"
     gene            988..3837
                     /gene="fdhF"
                     /locus_tag="VN496_11850"
     CDS             988..3837
                     /gene="fdhF"
                     /locus_tag="VN496_11850"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HXF17694.1"
                     /translation="MSLQTTPDYGTPKRISEKLVTLEIDGIEVSVPEGTSVMGAAMDA
                     GVRVPKLCATDSLEAFGSCRLCLVEIEGRKGYPASCTTTVAPGMKVRTQTPKLADLRR
                     GVMELYISDHPLDCLTCSANGNCELQDMAGVVGLREVRYGYEGENHLKTPKDTSNPYF
                     TFDPSKCIVCSRCVRACEEVQGTFALTIDGRGFASMVSPSENQPFMESECVSCGACVQ
                     ACPTATLMEKSVIEKGQADHSVTTTCAYCGVGCSFNAEMKGAEVVRMVPNKDGKANRG
                     HSCVKGRFAFGYATHPDRITKPMIRAKISDPWREVSWDEAISHAASEFKRIQGKHGRG
                     SIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKTTLGESAGTQD
                     FDSVMKSDVIMVIGANPTDGHPVFASRMKRRLREGAKLIVADPRTIDMVRTAHVEASY
                     HLQLRPGTNVALINSLAHVIVSEGLTKDDFIAARCDAKEFAKWKTFILESRNSPEEME
                     KTTGVPAAQVRGAARLYATGGNAAIYYGLGVTEHSQGSTMVIGIANIAMATGNIGHEG
                     VGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSDDKVRGQFEKQWGVKLDNEPGLRIPN
                     MFDASIDGSFKGLYIQGEDIAQSDPDTQHVTHALEALECVVVQDIFLNETAKFAHVFL
                     PGSSFLEKNGTFTNAERRISRVRKVMPPLAGKEDWEVTVALSNAFGYPMTYKHPSEIM
                     DEIALLTPTFKGVTYDKIDELESIQWPCNDAAPDGTPVMHVDEFVRGKGRFVVTEYVP
                     TEERTSGKYPLILTTGRILSQYNVGAQTRRTANNLWHSEDRLEIHPHDAEQRGIKDGD
                     WVGITSRAGETVLRSTITERVQPGVVYTTFHFPESGANVITTDNSDWATNCPEYKVTA
                     VQVSRVTQPSAWQKHFREFTETQLELMPQREASGA"
     gene            3839..4681
                     /gene="fdhD"
                     /locus_tag="VN496_11855"
     CDS             3839..4681
                     /gene="fdhD"
                     /locus_tag="VN496_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXF17695.1"
                     /translation="MTRRAFDLATAQRRSVRAVRLDTDSTCAVVEQIAEEAPIALVYN
                     GVPHVVVMATPIDLENLALGFSLTEAVIESASELGGIEVVPEPTGYSIYMSVPPERVM
                     AIEQRRRNMTARTGCGLCGAQTIEQAMRDVPKVKSGLRIARRAVARAMKLLPSRQALN
                     AATGATHAAAWANGDGELQLIREDVGRHNALDKLIGALATANIDTAQGFCVITSRASY
                     EMVQKAAMAGIGLLAAVSAPTALAVRIARESGVTLAGFVRGERGVVYADNAYRLTTDD
                     DWKT"
     gene            4678..4887
                     /locus_tag="VN496_11860"
     CDS             4678..4887
                     /locus_tag="VN496_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HXF17696.1"
                     /translation="MNIEHLVRMANDIGNFFKSEPDHSTAVDSVALHLKRFWDPRMRK
                     EIVAYVDNGGAGLLELVREAVMKLR"
     gene            complement(4919..>5149)
                     /locus_tag="VN496_11865"
     CDS             complement(4919..>5149)
                     /locus_tag="VN496_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17697.1"
                     /translation="LLVRAAAPKNATGRVYGVVYSGLDIGLSGAPLMFGLLMDAHHPG
                     WVFMGIGAFQALSLIAVMGVGESAAKRQIQAA"
BASE COUNT         1118 a         1456 c         1564 g         1011 t
ORIGIN      
        1 cccgtacgtg ctgatcgaag gtatggcgat ctgcggcatt gcggtcggag ccacccgcgg
       61 ttacatctat gtgcgttcgg aatacccgca cgcctgcaac atcctggacg atgccattac
      121 gctcgcgagg gaccagcact atcttggcga taacatcctg ggcagtgaca agtcgttcga
      181 gctggaagta cgcaaaggcg ccggcgctta catttgcggc gaagaaacat ccctgctgga
      241 aagccttgag ggcaagcgcg gcatggtgcg tttcaagccg ccattgccag cgatatccgg
      301 attgttcggc aagccgacgg tgatcaacaa cgtcattacg ctcgcttccg tgccgatcat
      361 ttttgcgaaa ggcgccgatc actatcggaa ttatggcctg gggcggtcgc gcggcacgtt
      421 gcccatccag cttgccggca acctgaaaca ttgcggtttg gtggaaaagg cgttcggagt
      481 gactttgcgc gagctgcttt acgactatgg cgggggttcg gcgagcgggc gccccctgcg
      541 tgccgtgcag gttggtggtc cgcttggagc ttacctgcca gaacagcagt tcgatacgcc
      601 attggactac gaggcattcg ccgcgattgg cgcaatggtc ggccacgggg gcatcgtcgc
      661 gttcgacgac accgtcgaca tggccagcat ggcgcgttac gccatggaat tctgcgccat
      721 cgaatcctgc ggcaaatgca cgccatgccg cattggatcg acccgcggtg tggaagtgat
      781 cgaccatatc gttgccggag atgagcgcga aaaacatatc gcgctgctgg aagacctgtg
      841 cgacacgatg attaacggtt cgctgtgtgc gttgggtggc atgacaccat ttccggtaca
      901 gagcgcactc aaatattttc cgaatgattt tcacgtggcg cctaaacgca aggttgcgtg
      961 acgctacggt tttggtagga gataacgatg tccctgcaaa caacacccga ttacggcact
     1021 ccgaagcgga tatcggaaaa acttgtcacg ctggagatcg acggcattga agtgagcgtg
     1081 ccggaaggaa cctcggtaat gggcgctgcc atggacgcgg gtgtccgcgt gcccaagcta
     1141 tgcgcgaccg acagcctcga agctttcgga tcatgccgcc tttgcctggt cgagatcgaa
     1201 ggccgaaaag gctatcccgc ctcctgtacg accacggttg ctccgggaat gaaggtgcgt
     1261 acgcagaccc cgaagctggc ggacctgcga cgtggtgtca tggagctgta catttccgac
     1321 catccgctgg actgcctgac ctgttcggcg aacggcaatt gcgaattgca ggacatggcc
     1381 ggcgtagtgg ggttgcgcga ggtgcgttat ggctacgagg gtgagaatca cctgaaaacg
     1441 ccgaaggaca cctccaatcc ctacttcaca tttgatccga gcaaatgcat cgtctgctcg
     1501 cgctgcgtgc gggcttgcga ggaagtgcag ggcacatttg cgctcaccat cgatgggcgt
     1561 ggattcgctt ccatggtctc gccaagcgaa aaccagccgt tcatggaatc ggaatgcgtg
     1621 tcttgcggcg catgtgtcca ggcgtgcccg accgcgactc tgatggagaa atccgtcatc
     1681 gagaaaggtc aggcggatca cagtgtcacc acgacttgcg cttattgcgg cgtaggctgc
     1741 tccttcaacg cggaaatgaa gggcgcagag gtcgtgcgca tggttcccaa caaggatggc
     1801 aaggccaatc gcgggcactc ttgcgtaaaa ggacgtttcg cgtttggcta tgccacgcat
     1861 ccggaccgca ttaccaagcc catgatccgc gccaaaatca gcgacccgtg gcgcgaagtt
     1921 tcctgggacg aggcgatcag ccatgccgcg tcggaattca agcgcatcca gggaaagcac
     1981 ggtcgcggct cgatcggtgg cattacttcc tcccgttgca ccaacgaaga aacttacctt
     2041 gtccagaagc tggtgcgcgc cgcattcggc aacaacaacg tcgatacctg cgcacgtgtc
     2101 tgccattctc caaccggtta cgggctcaaa accacgttag gcgaatccgc aggaacgcag
     2161 gacttcgatt cggtcatgaa gtcggacgtc atcatggtca tcggcgccaa tccgacggac
     2221 ggccatccgg tatttgcttc gcgcatgaag cgccggctac gcgaaggcgc gaagctgatc
     2281 gtcgctgacc cgcgcaccat cgatatggtt cgcaccgcgc atgttgaagc ttcatatcac
     2341 ctgcaactgc gtccgggcac caacgtggcg ctgatcaatt ccttggcaca cgtcatcgtg
     2401 agcgaaggtc tcacgaagga cgatttcatt gctgcacgct gcgatgcaaa ggaatttgcc
     2461 aagtggaaga cgtttatcct cgagtcgcgc aattctcctg aagaaatgga aaagactacc
     2521 ggcgttccgg cggctcaggt tcgcggcgca gccaggcttt atgcgacggg aggcaacgcg
     2581 gcgatttact acggtttggg cgtgactgag catagccagg gctcgaccat ggtcatcggc
     2641 attgcgaaca tcgccatggc gaccggcaac atcggacacg aaggagtcgg cgtgaatccg
     2701 ttgcgcggtc aaaacaacgt gcagggttcc tgcgatatgg gttcgttccc gcacgaattg
     2761 ccgggctatc ggcatgtttc cgacgacaag gtccgtggtc aattcgagaa gcaatggggt
     2821 gtgaagctcg acaatgaacc gggactgcgc attccgaaca tgttcgatgc atccatcgac
     2881 ggcagtttta agggcttgta cattcaaggc gaggacatcg cgcagtctga tccggatacc
     2941 cagcacgtaa cgcacgcgct tgaggccctg gagtgcgtgg tggtacagga tattttcctg
     3001 aacgagactg cgaaattcgc gcacgtgttt ttgccaggct cttcgttcct cgaaaagaac
     3061 gggaccttta ccaatgccga acgtcgcatt tcgcgcgtgc gcaaggttat gccgccattg
     3121 gccggcaagg aagactggga ggtgacggtc gccttgtcca acgcattcgg ttacccgatg
     3181 acgtacaagc atccatccga aatcatggac gaaatcgcgt tgctgacgcc aacgtttaag
     3241 ggcgtgacct acgacaagat cgatgaactg gagagcattc aatggccctg caacgacgct
     3301 gccccggacg gtacgccggt gatgcatgtg gacgaatttg tgcgtggcaa aggccggttc
     3361 gtggtcaccg aatacgtccc gacggaagaa cgcactagcg gcaagtatcc gttgatcctg
     3421 accaccggtc ggattctttc gcagtacaac gtgggcgctc aaacgcgccg cacggcaaat
     3481 aatctttggc atagcgagga ccggctggag attcatccgc atgatgccga gcagcgcggc
     3541 atcaaggacg gggactgggt gggcattacc agccgcgcag gagagacggt attgcgttcc
     3601 accattaccg agcgggtcca accgggtgtg gtctacacca cgtttcattt tccggagtcc
     3661 ggcgcaaacg tcatcaccac ggacaattcc gattgggcga cgaactgccc cgaatacaaa
     3721 gttactgctg ttcaggtcag ccgcgtgaca cagccctccg cctggcaaaa acacttccgg
     3781 gaattcactg agacccaact ggaattgatg ccgcagcggg aagcaagcgg ggcctgacgt
     3841 gacgcgcagg gcgtttgacc tagcgaccgc gcagcgccgc tcagtacgcg ctgtgcgact
     3901 ggatacagat tcgacttgcg ccgttgtcga acaaattgcc gaagaggcac cgatcgcgct
     3961 ggtctacaac ggcgtgccgc atgtcgtcgt gatggcgacg ccgatcgatc tcgaaaacct
     4021 tgcgctcggg ttcagcctta ctgaagccgt gatcgaatcg gcttccgaac tgggcggcat
     4081 cgaagtcgtt cccgagccga ccggatactc catttacatg agcgttccac ccgaacgggt
     4141 catggcgatt gaacaacgtc gtcgcaacat gacggcccgc accgggtgcg ggctatgtgg
     4201 tgcacaaacc atcgaacagg ccatgcgcga cgtgccgaag gtcaaatccg gcctgcgcat
     4261 cgcgcggcgg gcggtagctc gagccatgaa gctgctgcct tccagacaag cactgaacgc
     4321 agcgactggt gcaacgcacg cggcagcgtg ggcgaatggc gacggcgaac tgcaattgat
     4381 tcgcgaggat gttgggcgcc ataacgcgct ggacaaactc attggcgcac tggcgaccgc
     4441 gaatatcgac accgcacagg ggttttgcgt gattaccagc cgcgcaagtt atgagatggt
     4501 gcagaaagcc gccatggccg gcatcggtct gcttgcggcg gtgtcagcgc cgaccgctct
     4561 ggccgtacgt atcgctcgtg aatcgggagt tactctggca ggctttgtgc ggggagagcg
     4621 cggcgtagtc tatgccgata atgcgtaccg cctgacgacg gacgacgact ggaaaacatg
     4681 aatattgagc atctggttcg catggcgaac gacattggca atttcttcaa gtccgagccg
     4741 gatcacagta ctgccgtcga cagtgtggcg ctgcatctga agcgattctg ggacccgcgg
     4801 atgcgaaagg aaatcgttgc ttacgtcgac aacggcggtg cgggattgct ggaactggtg
     4861 cgcgaagcgg tgatgaagct gcgctgaaaa gcgaggacga tatccgtccc aagttcactt
     4921 acgccgcctg tatttgccgt ttggcggcgc tttcgcccac tcccatcacc gcaatcaggg
     4981 acagcgcctg aaaagcgcca atgcccatga acacccagcc aggatgatgt gcgtccatga
     5041 gcaggccaaa catcagtggg gcgccggaaa gcccgatgtc caggccggaa tacaccacgc
     5101 cgtacacgcg gccggtcgca ttcttaggcg cggctgctct taccagcag
//
LOCUS       DATMZQ010000368         4392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_838927, whole genome shotgun sequence.
ACCESSION   DATMZQ010000368 DATMZQ010000000
VERSION     DATMZQ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838927"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VN496_11870"
     CDS             <1..308
                     /locus_tag="VN496_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXF17698.1"
                     /translation="RCPWGKKAFTGYLGEERAAWKEYDASELVRRGRFAGTILVDQGL
                     ADKFLPEQLFPEAFESACRDAGQPLTLRMQDGYDHGYFFIATFMEDHLRHHAEQLKG"
     gene            312..1175
                     /locus_tag="VN496_11875"
     CDS             312..1175
                     /locus_tag="VN496_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXF17699.1"
                     /translation="MQKLLVYQSLWAMERRRPDGKEWDLAEKLNMIHAAGFDGCGVRF
                     FDYQYAREVTAFLRAHNMTWQAQCYPQKVDDLKPILEHVRELGADHINLQPDVRPYSI
                     QECIPYIEGWRRLAHDASIPVHIETHRDRMTTDLFFTMQLLDCFPDLRLTGDLSHYVV
                     GREFAWPISEVNHGHMRRIIDNCWGFHGRVASREQVQVQISFAHHKEWLDLFMGWWEY
                     GFRSWRRRAPKDATLTFLCELGPKEYAMTGADGYELSDRWQEALMLKDLVRQLWQRIE
                     HEESSPAARAA"
     gene            1240..1932
                     /locus_tag="VN496_11880"
     CDS             1240..1932
                     /locus_tag="VN496_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXF17700.1"
                     /translation="MADEHMIMAGRLLAAVLAGGLIGLERSFRGRPAGFRTHTLVCLA
                     STVLMLITVYEWQWIQLHAADSIRMDPTRMAQGIMTGIGFLGAGVIFKEGITVRGLTT
                     AASIWITAAIGILIGIGFYFPATATTALALFVLSIFRWIESRMRTQNYARLSIAFDRG
                     GVLDEPALRAELAQHGFDAAHLSYRVGESGNVFEYRMMIQTTRPENFGTLAASLATNR
                     LVKQFRLAPTGD"
     gene            complement(1982..2764)
                     /locus_tag="VN496_11885"
     CDS             complement(1982..2764)
                     /locus_tag="VN496_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXF17701.1"
                     /translation="MSTRNSTPDARYSAPALEKGLDVLELLAAESRALNLQDIARNLE
                     RSPNELFRMIDVLVRRGYLAREPDATYRLTLRLFELAHRHHPVDRLLDCAVPYMHELA
                     RWTGQSNHLCVHHDGRLVVLARAESPEPMSYSVRLGSHFPFHDDRVSARVLSAFQQGA
                     RREQFLAELAGSSRNTARRHKIAKRLEQIAKRGYEEGPSDTISGVVDICFPVFDHFSV
                     VASLNIVYMRHRDAKVSVPAARQRLSQCAQLISRALGWASQT"
     gene            2978..4057
                     /locus_tag="VN496_11890"
     CDS             2978..4057
                     /locus_tag="VN496_11890"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXF17702.1"
                     /translation="MRTLKQLKRTVAALGCLAAMSTFAADPSTFPAPTGDPAYVPAGA
                     KLELIFDGGCALTEGVAAGHDGMIYFSDITFTKFCKDPSGKYLQAGNIWKYNPKNGET
                     TIFRSPSGMSNGIKFDRDGNMIAALGADYGGRMLVRTDMKTGKTIILSGLYNGRPYNA
                     LNDVTIDEKGRIYFSDPRYLGHEPIYQDGYAVYRLDTDGTVTRVATDCGKCNGVLISP
                     DQKTLYIIGNDNGWFEFQNLKEGEKTLQGHHQLQAYDVAADGSLSNRRVLIDYTARDK
                     PCSGPDGMVADAEGHIWLASRCEYRPGVQVLDAQGKELAYISTGKELPTNVGFGRGAD
                     SNLLYLTSGKSLYKIRVGKKGYQLP"
     gene            4134..>4392
                     /locus_tag="VN496_11895"
     CDS             4134..>4392
                     /locus_tag="VN496_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17703.1"
                     /translation="MSSVQRAVTGKGNVLTEAQVEAFQKNGYHYPVRALSAPEADACR
                     QTIENFEFEHGLVMKTPYRNKPHMVFKWANRMIRDPRIVDAV"
BASE COUNT          973 a         1219 c         1306 g          894 t
ORIGIN      
        1 tgcggtgccc atggggaaaa aaagcgttca ccggttatct cggcgaagaa cgcgcggcat
       61 ggaaggaata cgacgccagc gaacttgtca ggcgcggtcg atttgccgga accattctcg
      121 tggatcaggg gctagccgac aaatttctgc cggagcaact ctttcccgaa gcatttgagt
      181 cggcgtgccg agacgcggga caaccgttaa cgctacgcat gcaggatggc tacgatcacg
      241 gctatttttt tattgcgacg ttcatggaag atcatctccg gcatcatgca gaacagttga
      301 aagggtgaat catgcaaaag ctgcttgtct accagtcgct atgggccatg gagcgccgcc
      361 gtccggatgg aaaagaatgg gacctggcgg aaaaactgaa catgatccat gccgccggtt
      421 ttgatggctg cggcgttcga ttcttcgact accagtacgc cagggaagtc accgctttcc
      481 tccgtgccca caacatgacc tggcaggcgc agtgttatcc acagaaggtt gatgatctga
      541 aacccatcct cgagcatgtg agggaactgg gtgccgacca catcaacctg cagcccgatg
      601 tgcggcccta ttcgatacag gaatgcatac cgtacatcga gggctggcgc cggcttgcgc
      661 acgacgccag cattccggtg cacatcgaaa cacaccggga ccggatgacc accgatctgt
      721 tcttcaccat gcagctgctg gattgctttc cggatcttcg gctgactggc gatttgtcac
      781 actatgtcgt gggccgcgaa ttcgcctggc cgatcagcga agtcaaccat gggcacatgc
      841 ggcgcatcat cgacaattgc tggggtttcc atggccgggt tgccagccgt gagcaagtgc
      901 aggtacagat cagcttcgcc caccacaagg agtggctcga tctgtttatg ggttggtggg
      961 aatacggctt ccgcagctgg cgcaggcgtg cgccgaaaga tgcgacgctg acatttctgt
     1021 gcgaactggg gccaaaggaa tacgccatga ctggcgccga tggctatgaa ttgtccgatc
     1081 gttggcagga ggccttgatg ctgaaggacc tggtgcggca actttggcag cgcatcgagc
     1141 atgaggaatc cagccctgcc gcgcgagcgg cgtgacttaa acatgatgta atgcggtggc
     1201 tttcaccgtt gcggtgaagc ttcatcaatg ggaacggcaa tggctgacga gcacatgatc
     1261 atggcgggca ggcttttagc cgccgtttta gctggaggac tgatcgggct cgagcggagt
     1321 tttcgcggac gcccggccgg cttccggacc catactctgg tctgcctggc ttcgaccgtc
     1381 ctgatgctga tcaccgttta cgagtggcaa tggatccaat tgcatgctgc cgacagcatt
     1441 cgcatggatc cgacgcgcat ggcccagggc atcatgaccg gcatcggatt cctgggcgcg
     1501 ggggttatct tcaaggaggg catcacggtg cgcggtctga ccaccgctgc ttcgatctgg
     1561 attacggctg cgattggcat tctgatcggc atcggctttt attttccggc gaccgcgacc
     1621 acggcgctgg ccttgttcgt actgtcgata ttccgctgga tcgagtcccg catgcggacg
     1681 caaaactatg cacgcctcag catagctttc gaccgcggcg gtgtgctgga tgaaccggca
     1741 ttgcgggcgg aactggcaca gcatggattc gatgcggcgc acttgagtta tcgggtcggt
     1801 gagagtggca acgtttttga ataccggatg atgatccaga ccacgcgccc ggagaatttc
     1861 ggcacgcttg cagccagtct tgcgaccaat cgactggtga agcaattccg gcttgcacct
     1921 accggcgatt gaactgcacg ccatagtctc gcatcgcgat gtgatcccga tattgcccca
     1981 atcaagtttg ggacgcccaa cccagggccc gggaaatgag ctgcgcacac tgactcaatc
     2041 gctgtcgtgc agcgggcact gaaactttgg catcgcgatg gcgcatgtag acgatgttca
     2101 gcgaagcgac aacgctgaaa tgatcgaaaa ccgggaaaca gatatccact acgccgctga
     2161 tggtgtcgct cggaccttcc tcatagccgc gttttgcaat ctgctccaat cgcttggcga
     2221 tcttgtgtcg gcgcgccgtg ttgcggcttg atcccgccag ctcggcaagg aactgttcgc
     2281 gtcgagcccc ctgctggaag gcggaaagca cgcgagcgga tacccggtca tcgtgaaaag
     2341 ggaagtgcga gccgagccgc actgaatagc tcatcggctc cggcgattca gcccgcgcaa
     2401 gcaccacgag gcggccatcg tggtgtacgc acaagtgatt cgattggcct gtccagcggg
     2461 caagctcatg catgtagggg accgcgcaat ccagcagccg gtccaccggg tgatgccgat
     2521 gggccagctc gaataaacgc aaggtaaggc gataagtcgc atctggttct cgggccagat
     2581 agccgcggcg caccaggacg tcaatcatgc ggaacagttc gtttggcgaa cgctctaaat
     2641 tgcgtgcaat atcctgcagg ttcagcgcgc gtgattcggc cgccagcagt tccagcacat
     2701 ccaggccctt ttccagagcc ggagcggaat agcgcgcatc aggagtggaa tttcgtgttg
     2761 acaagtggtg cgccggtttt tatatttgaa atggcatttt atatatgaag cattgctgga
     2821 aagtaaattg tgcagtgcac atgccacact ggcgtcaaag gtagttgtcc tgctccaatc
     2881 cacgcgaacg gcccgcatgc ccataaacga tgcgaaagcc tcgtagtcca taacagaacg
     2941 agtggctaaa cattcgatag atatccggag gaaaaaaatg agaaccctga aacagctcaa
     3001 acgcaccgtc gccgcgctcg gctgcctggc tgcgatgtcg acttttgcag ccgatccttc
     3061 aacctttccg gccccgaccg gcgatcccgc atacgtgccg gccggtgcga aactggagtt
     3121 gatctttgat ggtggctgcg cgctgacgga aggtgtcgcc gcagggcacg atgggatgat
     3181 ttatttcagc gatattacct tcaccaaatt ttgcaaggac ccgtccggca agtatcttca
     3241 ggcgggaaac atctggaagt acaaccccaa gaacggcgaa acgaccatct tccgcagccc
     3301 atccggcatg tccaatggca tcaagttcga ccgcgacggc aacatgatcg cggcgctggg
     3361 cgcggattac ggcggccgca tgctggtcag gaccgacatg aagaccggca agacaatcat
     3421 cctgtccggc ctgtataacg ggcggcctta taacgcactc aacgacgtca ccatcgacga
     3481 gaagggccgc atctactttt ccgatccgcg ctatcttggc cacgaaccga tttaccagga
     3541 tggctacgcg gtgtaccggc tcgataccga tggtaccgtg acgcgcgtag cgaccgactg
     3601 tggcaagtgc aatggcgtac tgatttcacc cgaccagaag acgctttata tcatcggcaa
     3661 cgacaacggc tggttcgaat tccagaacct gaaggagggt gagaaaacgt tgcagggcca
     3721 ccatcaattg caggcctacg atgtggcggc cgatggtagt ctaagcaacc ggcgcgtcct
     3781 gattgactac accgcgcgcg acaaaccgtg cagtggtcct gacggcatgg ttgcggatgc
     3841 ggaaggccat atctggctgg catcgcgctg cgagtaccgg ccgggcgttc aagtgctcga
     3901 tgcccagggc aaggagctcg cctacatttc caccggcaag gaactgccca ccaacgtcgg
     3961 atttggtcgg ggagccgact cgaatttgct gtatctgacg tcgggcaaaa gcctttataa
     4021 aatccgggtg ggcaagaaag gctatcagct gccctgaggc ttcggaaaga tcgtaagcgg
     4081 tcgcgggggc ggtaacgcct ctgcgacttt tttcttaatg aaggagaagt atcatgagca
     4141 gcgtgcagcg agccgtcacc ggcaaaggaa atgtcctgac cgaggcgcaa gtggaagcgt
     4201 tccagaaaaa cggctaccac tatcccgtgc gcgcgctttc cgcaccggag gccgatgcct
     4261 gcaggcaaac aatagagaac tttgaattcg agcacgggct ggtcatgaag acgccctatc
     4321 gcaacaagcc ccacatggtg ttcaaatggg ctaaccggat gattcgcgat ccgcgcatcg
     4381 tggatgctgt gg
//
LOCUS       DATMZQ010000369         8350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2219151, whole genome shotgun sequence.
ACCESSION   DATMZQ010000369 DATMZQ010000000
VERSION     DATMZQ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219151"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="VN496_11900"
     CDS             complement(<1..1732)
                     /locus_tag="VN496_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXF17704.1"
                     /translation="MDAYWRAANYLSVGQIYLCDNPLLRQPLKLDHIKPRLLGHWGTT
                     PGLNFMYVHLNRVITQQDLNVIYVTGPGHGGPGLVANTYLEGTYSELYPNVSQNEAGI
                     KKLFKQFSFPGGIPSHAAPQVPGSINEGGELGYSLAHAYGAAFDNPDLLVACVVGDGE
                     AETGPLATGWHSNKFLNPVHDGAVLPILHLNGYKIAGPTLLARIPHPELEALLRGYGY
                     RPYFVEGDEPARMHRLMAQTLDTVVAEIHAIQRDARENGFRSRPRWPMIVLRSPKGWT
                     GPLAVDGKAVEGTFRSHQVPLAELATNAGHLEILERWMKSYRPDELFDVDGRFRAELA
                     ALAPKGDRRMGANPHANGGLLLRDLKLPDFRDYAVRITVPGDVAAESTRIQGQFMRDV
                     FKQNADARNFRVFSPDETASNRWNAVFEVTDRCSTADIVPGDENVAPDGRVMEMLSEH
                     QCEGWLEGYLLTGRHGFLSSYEAFIHIIDSMFNQHAKWLDVAGQIPWRRPIASLNYLL
                     SSHVWRQDHNGFSHQDPGFIDIAMNKSARVVRVYFPPDANSLLSVTDHCLRSRNYVNV
                     IVAGKQPEAQW"
     gene            2075..3892
                     /locus_tag="VN496_11905"
     CDS             2075..3892
                     /locus_tag="VN496_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HXF17705.1"
                     /translation="MKNHPVRYSVFLSNPEAHLFEVHCSIEDPDPMGQKFSLPTWIPG
                     SYLIREFAKHVVRIGALGNRRKLRMVKLDKNTWQVEPADGPVVVIMEVYAWDLSVRGA
                     HFDTTHAFFNGPALFLQVHGHAETPVLVDIQPPKGTKYRNWRIATAMRRKGARPYGFG
                     TYEAANYDELIDHPVEIGTFELATFKAAGVQHDVAVTGRHRGDLRRLVKDLKKLCETQ
                     IAFFGTPAPMDRYVFLITTVGDGYGGLEHRSSTALLCARNDLPQPNVVTASGGYRTLL
                     GLASHEYFHTWNVKRIKPAAFTPYDLDRENYTTLLWAFEGFTSYYDDLLLVRAGLISH
                     ATYLDTLARNITSLLRLGGRKRQTIAESSFDAWIKYYRSDENTPNAGVSYYLKGSLVA
                     LCLDLLIRAQTGRRKSLDHVMRGLWKRHGMTGIGVPEDGIEQVAEQITGLKLTRFFNQ
                     ALHTTADLPLQKLLKTVGVEIRQRPARSGMDRRDQMSRKERQASRGVALGVRTGNDGG
                     ELKLSQVFTGSAAQKAGLAAGDIIIAIDGLRVTPDNVDAHLGRYRAGETIPVHAFRRD
                     ELLVLPVKLEAAKLDAWSLSVVGTSKETVRARKSWLGSA"
     gene            complement(3906..4760)
                     /locus_tag="VN496_11910"
     CDS             complement(3906..4760)
                     /locus_tag="VN496_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6662 family protein"
                     /protein_id="HXF17706.1"
                     /translation="MASARQKFVPRALTPAVAMLGAMLFASMAISPAAADERFFTYSQ
                     DADVIPKGGTEFEQWLTLREGHPGGDRQFDQYLWDFREEIEHGFTDRLSGSLYLNFNQ
                     NQIVARQPGLTDSSDFSFKGVSGELKYQLLNPNTQPIGLALYFEPTYSGSEREIEYKL
                     IVSKNIADKWVLAANATFEQEWEDEDGGTEKESVAEFTLGAAYRITPNWSVGLEGRYH
                     AVYEGSSLNDHLGTGIFVGPNVHYATPKWWATLTFLPQGSGHPSDGGINRTEHQTFET
                     RLIFGINL"
     gene            complement(4918..5667)
                     /locus_tag="VN496_11915"
     CDS             complement(4918..5667)
                     /locus_tag="VN496_11915"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXF17707.1"
                     /translation="MPGVSITQEAGVRYLQFGAHWIQGAMRVNRPWSLELEYTRDMMF
                     PLLLRPQTWPRSVLLIGLGAASLTKFLHRYRPGARLDVVEIDPLVVMAAWQFFGLPAE
                     SRRLRIAVDDGYRFVATARRRYDLILVDGFDAKVSAGDLDSPVFYRNCHARLTAQGIL
                     ATNLVSRRGKPKASIDRIGRIFRGGLVAFPRNEANTIVLAKAGDSVRVTRKALFSAAR
                     TLKESSGLDLTGTITGLLNEYSEDDLVFRAR"
     gene            5945..8206
                     /locus_tag="VN496_11920"
     CDS             5945..8206
                     /locus_tag="VN496_11920"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HXF17708.1"
                     /translation="MRLSLRFVLPLLAVLAAVAYGVTQLADQLTLRWFVRDLDARTVV
                     IAKAVDESLRDLVPAGQGKRIVKLFESMVRDERLLAMGFCNEQGELAYKTFTFPAAVT
                     CRTLQKLTPGAGTVLQLPQGSLHAAISPILGTEGNELGKLVLLHDMGFIEHRSADTRR
                     YVFYLFAALGVVISLVTVAVAQISWWGWMKGMRVMLTGEWLAMPDSGPAEMRPVARDL
                     QALIRDLGRDRRMRDESQISWNRDALRTILREDLRGDEFLIVSNREPYIHVRAPDNSI
                     EVQRPASGLITALEPVMLACSGTWIAHGSGSADRDVVDRKDHIAVPPGKETYDIRRVW
                     LSEAEENGYYFGFANEGLWPLCHIAHTRPVFRPLDWEAYVEVNRKFARVIIEEARTED
                     PVVLVQDYHFALLPAMIRERLPDATIIAFWHIPWPNPEAFGICPWADQILAGMLGSSI
                     LGFHTRFHCNNFLDTVDRFLEARVDRETNGVTHMGMRTLVKRYPISIEWPLSGLQGLP
                     SVKECAEWLRQRHGLAPELRLAVGVDRLDYTKGILERFLALERLFELEPEWVGKLTLL
                     QIGAPSRATIPEYRHFLEDVRDEAARINERFSREGYAPIILRMENVGAKEVYRYYRGA
                     DVCVVSSLHDGMNLVAKEFVAARDDEQGVLILSEFTGASRELAEALIINPYNMDQFAS
                     ALRVALTMPAGEQRDRMRSMRSLIQEFNVYRWAGRMLLDASVMRRRTRVLRHGTDVSY
                     GKRLLRRAREVAS"
BASE COUNT         1739 a         2414 c         2435 g         1762 t
ORIGIN      
        1 gccactgtgc ttcgggctgc ttgccggcga caatcacatt tacatagtta cggctgcgca
       61 ggcaatgatc cgtcaccgac aacaacgagt tggcatccgg cggaaaataa acgcgcacca
      121 cgcgcgctga cttgttcatc gcgatgtcga tgaaaccggg atcctggtgg ctgaacccgt
      181 tgtgatcctg tcgccatacg tgagacgaga gcagatagtt cagggaggca atcggccgcc
      241 gccatggaat ctgtcccgcc acatcaagcc atttcgcatg ctgattgaac attgaatcga
      301 tgatgtggat aaacgcttcg taactcgaca gaaaaccatg gcggccggtg agcagatagc
      361 cctccagcca gccctcgcac tgatgctcgc tcaacatttc catcacgcgc ccatccgggg
      421 cgacgttctc atcacccgga acaatgtccg cggttgaaca gcgatcggtc acctcgaaca
      481 cggcattcca ccggttcgac gcggtttcat cgggactaaa gacgcggaaa ttacgtgcat
      541 cggcgttttg cttgaacacg tcgcgcatga actgaccctg aatgcgcgtt gattcggccg
      601 ccacatcccc tggaaccgta atgcgcacag catagtcgcg gaaatccggc aactttagat
      661 cgcgcagtag aagaccgcca ttggcgtggg ggttggcacc catcctgcgg tcgcctttgg
      721 gtgccagcgc ggcaagctcc gctctgaatc gtccgtcaac atcaaacagc tcgtcgggac
      781 gatagctttt catccagcgt tccagaattt ccagatggcc tgcattggta gcgagttccg
      841 cgagcggtac ctgatgagaa cgaaatgtgc cttccactgc tttgccatcc acggccaacg
      901 gtcccgtcca gccttttggc gagcgcagca cgatcatcgg ccagcgcggg cggcttctaa
      961 atccgttttc tcgcgcatcg cgctggatcg catgaatttc cgccacgaca gtatccaggg
     1021 tctgcgccat cagccggtgc atcctggccg gttcgtctcc ttccacaaag tacggccggt
     1081 aaccgtagcc gcgcaacaac gcttccagct cggggtgcgg aatgcgcgcg agcaatgttg
     1141 gaccggcgat cttgtagcca ttcagatgca ggatgggcag cacggcaccg tcgtgcaccg
     1201 ggttcagaaa cttgtttgaa tgccaaccgg tcgcaagcgg tccggtttcc gcttcaccat
     1261 cgccaacgac gcaggccacc agcaagtccg gattgtcgaa tgcagcgcca tacgcatgcg
     1321 ccagcgagta gccaagctcg ccgccttcat tgatggagcc aggcacttgg ggtgccgcgt
     1381 gactgggaat tccgccggga aaggaaaatt gcttgaacag ttttttgatg ccggcttcat
     1441 tttgcgacac attggggtac agctcgctat aggtcccttc caggtatgtg ttcgcgacca
     1501 ggcctggtcc gccatggccc ggcccggtaa cgtagattac gttcaaatcc tgctgggtga
     1561 tgacgcggtt cagatgaaca tacataaaat tcaatcctgg cgttgtgccc cagtggccaa
     1621 gcagtcgcgg cttgatgtgg tcaagcttca ggggctgccg taacaacggg ttgtcgcaca
     1681 ggtagatttg cccgaccgac aggtaattgg cggcccgcca gtaggcgtcc attttttgca
     1741 gcaactggtc cggaagcgcg tctgaagata cgccctcggg gacctctttc accacgacat
     1801 tgcctgcgtc gtccaattta cgctcccaac aaaaacggat ggcggtgcgg gcgccgtgct
     1861 tatcgcacta tggacctgcc acgtcaggta cggacctcct gagcggagtg aagtttcgga
     1921 acatgcgacg ctttgcgcaa tacaggcaac tgtggcgctg gttcttgatc gggcggacaa
     1981 agcactgttc gggaagctcg gtacggcttc acgcagcttg acgaaattga ttaagcccgc
     2041 cataaagccg gacgcacatt tttcgccgcc cccgatgaaa aatcaccctg tccgctactc
     2101 ggttttcctg agcaatccgg aagctcacct gttcgaagtc cattgcagca tcgaggatcc
     2161 ggatccaatg gggcagaaat tctcgctgcc aacctggatt ccaggcagtt atctgatccg
     2221 tgaatttgcc aagcacgttg tgcgcatcgg cgcactaggc aatcgcagga aattgcgcat
     2281 ggtaaagctg gacaaaaaca cgtggcaggt tgaaccggcg gatggtccgg tggttgtcat
     2341 catggaggtc tatgcctggg acttgtcggt gcgcggtgct cattttgata cgacgcacgc
     2401 tttcttcaac ggcccggcat tgtttcttca ggttcacggc catgcagaga cgcctgtgct
     2461 ggtcgatatc cagccaccca aagggacgaa gtaccgcaac tggcgcatcg cgacagcaat
     2521 gcggcgcaaa ggcgcccggc cttacggatt cggcacttac gaagcggcca actacgatga
     2581 actcatcgac catccggtgg aaattggcac gtttgaactc gcaacattca aggccgccgg
     2641 cgtgcagcac gacgtcgccg tcaccggccg gcatcgcggc gatctgcgac gtttggtgaa
     2701 agacctgaag aagctgtgtg aaacgcaaat cgcatttttt ggtacaccgg ccccaatgga
     2761 ccggtatgtg tttttgatta caacggtcgg cgacggttat ggcggcctcg aacatcgttc
     2821 ttccacggcg ctgctgtgtg cacgcaacga tctgccgcaa ccgaacgtcg tgaccgcgag
     2881 cggcggctac cggacgttgc tgggcctcgc gagccacgag tactttcata cctggaacgt
     2941 gaagcgcatt aaacccgccg catttactcc ttatgatctg gaccgcgaaa actacaccac
     3001 gctgctatgg gccttcgaag ggttcacgtc gtattacgac gacttgttgc tggtgcgggc
     3061 gggactgatt tcgcatgcaa cctatctcga cacactggcg cgcaacatta cttcattgtt
     3121 gcgtctcggc ggccgcaaaa ggcagaccat tgcggaatcc agctttgatg cgtggatcaa
     3181 gtattaccgc agcgatgaga acacgccgaa tgctggcgtg agctattacc tcaaaggcag
     3241 tctggtggcg ttgtgcctgg acttattgat ccgtgcgcag accggcaggc gcaaatcatt
     3301 ggaccacgtg atgcgcggac tgtggaaacg ccacggcatg acaggcattg gcgttcccga
     3361 ggacgggatc gaacaggtgg cagagcagat caccggactc aagctcacgc gtttcttcaa
     3421 ccaggccttg cacacgacgg ccgacctgcc actgcaaaaa ctgctcaaga ctgtaggcgt
     3481 ggagatacgg caacgcccgg cccgctccgg catggaccgg cgcgaccaaa tgtcacgcaa
     3541 ggagcggcaa gcctcgcgcg gcgtcgcgct cggcgtacgc accggcaacg atggcggtga
     3601 actgaaactg agccaggtct ttacgggaag tgcggcgcag aaagccggcc ttgccgcggg
     3661 cgatatcatc atcgccatcg atggcttgcg ggtgacgccg gacaatgtgg atgctcacct
     3721 tggccgttac cgggccggcg aaaccattcc ggtgcacgcg ttccgccgcg acgagttgct
     3781 cgtgttgccg gtcaagcttg aggcggcgaa actggacgcg tggtccctga gcgtcgtggg
     3841 aacctccaaa gaaactgtcc gggcgcgcaa aagctggttg ggaagcgcct gatgattgcc
     3901 gaacgctaga gattgatgcc gaaaattagc cgggtctcga aagtctgatg ctccgtccga
     3961 ttgatgccgc cgtcgctggg atgtcccgaa ccctgcggca gaaacgtaag cgtggcccac
     4021 cacttgggtg ttgcataatg aacatttggg ccaacgaata tgccggtacc aaggtgatcg
     4081 ttgagtgatg agccttcgta taccgcgtga tagcgcccct ctagcccgac ggaccaattc
     4141 ggcgttatgc gatatgcagc accgagtgta aattccgcga ccgattcttt ttccgtgccg
     4201 ccgtcttcat cttcccattc ctgctcgaat gtggcattag ccgcgagcac ccatttgtcc
     4261 gcaatattct tggaaacgat cagcttgtat tcgatctctc tttcgctgcc actgtaggtg
     4321 ggctcgaaat acagcgccag gccgatcggt tgggtgttcg ggttcagcaa ttggtacttc
     4381 agctcgcccg atacgccctt gaatgaaaaa tcgcttgaat cggttaggcc aggctggcgc
     4441 gccacgatct gattctggtt gaagttcagg tacagcgagc cgctcaaccg atcggtgaat
     4501 ccgtgttcta tttcttcgcg gaaatcccaa agatattgat cgaattgacg gtcgccgccg
     4561 ggatggcctt cgcgcagggt cagccattgc tcgaactcgg tgccgccttt tggaattaca
     4621 tcggcatcct gactgtaggt aaagaaccgc tcatccgcag ctgccggcga tatagccatc
     4681 gatgcgaaca acattgcgcc gagcatagca acagcgggcg ttaaagcgcg gggaacaaac
     4741 ttctgccttg cggaagccat gcgaatctct cctgaatgag tggacgacac aaacgcgagg
     4801 cagatactaa taggaatgag tcgcattaag caaatatatt cgttaataat ttgattcaac
     4861 gcacaaagtt gtcggtcaag gcgccccagc aaggtctttg ggttggcaca agccggctca
     4921 gcgagcccga aataccagat cgtcctctga gtattcgttg agcagtccgg tgatcgtgcc
     4981 ggtcaaatcc aggccgctgc tttccttaag cgtgcgggcg gcgctgaaca gcgctttcct
     5041 tgtgacgcgc actgaatcgc ccgctttggc cagcacgatt gtgtttgctt cattgcgcgg
     5101 gaatgcgacc aggccgcccc ggaaaattct gccaatgcga tcgatgctcg cctttggttt
     5161 cccgcgccgg ctcaccagat tggtcgccag aattccctgc gcggtaagcc gcgcatggca
     5221 attgcggtag aacacgggcg aatcgaggtc gcccgcactg actttcgcat cgaagccatc
     5281 gaccaatatc aggtcgtacc ggcggcgtgc cgtcgccacg aatctgtagc cgtcgtcgac
     5341 cgctatgcgc aggcggcgac tctctgccgg gagtccgaag aattgccatg cagccatcac
     5401 caccagggga tctatttcca cgacatcgag ccgcgccccc ggccggtaac ggtgcaggaa
     5461 tttagtaagc gacgcagccc caagaccgat caacagcacg ctacgcggcc atgtttgcgg
     5521 tcgaagcaat aatgggaaca tcatgtcgcg cgtgtactcg agttcaagcg accacgggcg
     5581 gtttacgcgc atcgcgccct ggatccagtg cgcgccgaac tgcaggtaac gcacgcctgc
     5641 ttcctgtgtg atgctgacac caggcatatc agccgcggtt caggactttg agaaggaagt
     5701 tcacagcaga agcggaatcc aaaatgcaag ggatcgcgat cttaccaaac gcgtgagcgt
     5761 gagaaggttt gaaggtctgt atttttcgag gcccagtatt tgtattgtcg gctgtgccct
     5821 ggcagcaaat cacggaactt gtgcctttgc tgcactgtct gccaccttac ttaggttgtt
     5881 tccgagcgcc ggccctgtgg tccgcgcttg cttgcgcgca gagtttgctc gattggaggt
     5941 gcaaatgaga ctttcactgc gtttcgtcct cccgttgttg gctgtgctgg ctgctgtcgc
     6001 ttacggcgtc acgcagcttg ccgatcagct caccctgcgg tggttcgtgc gtgacctcga
     6061 tgcgcgcacc gttgtcatcg caaaagcggt agacgaatcg ctgcgtgatc ttgttcctgc
     6121 cggacagggc aagcgcatcg tcaagctatt cgaaagcatg gtgcgcgacg agcgtctgct
     6181 tgccatgggt ttttgcaacg agcaaggcga attggcctac aaaaccttca cttttcctgc
     6241 cgcagtaact tgccgcacgc ttcaaaagct cactcccggt gccggcacag tgctgcaatt
     6301 accgcaaggc tcgttgcatg cagccatttc gccgatcctt ggcacagaag gcaacgaact
     6361 gggcaagctt gtgctgctac atgacatggg gtttatcgaa catcgcagcg cggacacgcg
     6421 gcgttatgtg ttttacctgt tcgctgcgct gggcgttgtg atttccctgg tgacggtcgc
     6481 cgtggcacag atcagctggt ggggctggat gaagggtatg cgcgtgatgc ttaccggaga
     6541 atggctggcg atgccggatt cgggaccagc ggaaatgcgt ccggtcgcga gagacctgca
     6601 ggcgctgata cgcgatctcg gacgtgaccg ccgcatgcgc gatgagagtc agatcagctg
     6661 gaaccgcgat gcattgcgta ccatcctgcg ggaagacctg cgcggtgacg agttcctgat
     6721 tgtctccaat cgcgagcctt atatccatgt gcgggcgcct gacaattcga ttgaggtaca
     6781 gcgtccagcg agcgggctca tcacggcgct cgaaccggtc atgctggcat gctcgggcac
     6841 ctggatcgcg cacggcagcg gatcggcgga ccgcgatgtc gtggacagaa aggaccacat
     6901 cgccgtgccg cccggcaagg agacttatga catccgcagg gtatggctca gtgaggccga
     6961 agagaacggc tattacttcg gcttcgcgaa cgaaggattg tggccgctat gccatatcgc
     7021 gcacacgcgc ccggtatttc gtccgcttga ctgggaagcc tatgtagagg tcaaccgcaa
     7081 gttcgctcgc gtgatcattg aggaagcgcg cacggaagac ccggtagtgc tggtgcagga
     7141 ttatcatttt gcgctgttgc cggcaatgat tcgcgaacgc ctgcccgatg ccaccatcat
     7201 agcgttctgg catattccat ggccgaaccc ggaggcattc ggaatctgcc cgtgggcaga
     7261 ccagatcctc gcaggcatgc tcggcagcag cattctggga ttccacaccc gcttccactg
     7321 caataacttc ctcgacacag tcgaccgctt cctcgaagcg cgtgtggatc gcgagaccaa
     7381 cggtgtcacg cacatgggca tgcgcacgct ggtcaagcgc tatccgattt ccattgaatg
     7441 gccgctgtca gggctgcaag gcctgccctc cgtcaaggaa tgtgcggaat ggttgcgcca
     7501 gcgccatggc ctcgctccgg aactcaggct cgcggttggc gtggacagac tcgactatac
     7561 caagggcatt ctggagcgct ttctggcact ggagcgcctg tttgagttgg aacccgagtg
     7621 ggtgggcaag ctgacactgc tgcagatcgg cgcgccgagc cgcgcgacca ttccggaata
     7681 ccgccatttc ctcgaagacg tgcgcgatga agcggcccgc atcaatgagc gcttcagcag
     7741 agagggctat gcacccatca ttctgcgcat ggaaaacgtc ggcgccaaag aagtctaccg
     7801 ttattaccgc ggggcggacg tctgcgtcgt cagcagcctg catgacggca tgaatctcgt
     7861 cgccaaggaa ttcgtcgccg cacgcgatga cgaacagggc gtactgatac tcagcgagtt
     7921 taccggtgct tcgcgcgagc ttgctgaagc gctgatcatc aatccgtaca acatggacca
     7981 gttcgcttcc gcgttgcggg tggcgttgac tatgccggcc ggcgagcagc gcgatcgcat
     8041 gcgcagcatg cgttctctga ttcaggaatt caatgtctac cgctgggcag gccgcatgct
     8101 gctggacgct tcagtcatgc gccggcgcac ccgcgtgttg cgccatggca cagacgtttc
     8161 ctacggcaag cgcttgcttc ggcgcgcacg ggaagtagca tcatgaacaa ccgaaatgcg
     8221 ctgctcgatc acgagcgcgt gccgcaattt gccgacaact gggccctctt tctggatgta
     8281 gatggcacgt tgctcgatat cgcccaaaat ccggacggag ttacggtttc aagcgatctg
     8341 tactccctgc
//
LOCUS       DATMZQ010000370         8462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2021868, whole genome shotgun sequence.
ACCESSION   DATMZQ010000370 DATMZQ010000000
VERSION     DATMZQ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021868"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VN496_11925"
     CDS             <1..233
                     /locus_tag="VN496_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17709.1"
                     /translation="TRASTIQRARVMKHYLQAVAPACTEKISQQTDAMHGVVSASQRL
                     IEDPNGYRSFCENGIDCRFVLGITEKSRVLRI"
     gene            complement(251..1387)
                     /locus_tag="VN496_11930"
     CDS             complement(251..1387)
                     /locus_tag="VN496_11930"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF17710.1"
                     /translation="MNKQLLGGLSAAAFLRRHWQKKPLLVRNAIPHFDGIQDFGALVE
                     FASRQDCESRLVVRHGTRWTVEHGPFHRKRFSRLPARNWTLLVQGLESMLPAARKLLS
                     NFDFIPYSRLDDVMVSYAAPGGGVGPHFDSYDVFLLQGPGQRRWRVSGQRDMALVEGA
                     PLKLLKRFLPEGECLVSAGDMLYLPPACAHEGIAVDCCFTYSIGFRAPSHQELTSQFL
                     VHLEDKAQATGRYSDPDLLPQAHPARLGSSMVAKVERLLANTRWQQRDVAEFLGIYLT
                     EPKARVVFKPPHQRLSEAAFSMAAKKRGVELALPSSMLYSRATHFMNGESYTTVKAVP
                     VLRRLADTRRLAPLQVPIDGAAMDLLYRWYHAGYIVLSQPGRDD"
     gene            1479..1949
                     /locus_tag="VN496_11935"
     CDS             1479..1949
                     /locus_tag="VN496_11935"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXF17711.1"
                     /translation="MLKVGEVAPTFDLPDGDMNVVSLSAYLGVNNVVLYFYPKDDTPG
                     CTREAIDFSDREDDFLDLDTVVLGVSMDDCMSHGAFRDKHGLAVKLLADTEGEVCEKY
                     GVLQEKKVEGKGERRGILRSTFVIDKSGALRNVLYGVNARGHAADVLNLVKELK"
     gene            1952..2431
                     /locus_tag="VN496_11940"
     CDS             1952..2431
                     /locus_tag="VN496_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXF17712.1"
                     /translation="MQISKDTVVSLNYELSDLKGKVIEKTQAPITYLHGGYDGIFPLV
                     EVALQGKSIGDKCNVTLKPDDAFGEYEKDMVRTEPKSLFPENISVGMQFEGAIEGSDD
                     YTVYTVTEIQGEKIVVDGNHPLAGQTLVFSGIVTGVRPATHEELHHGHVHGPDGHHH"
     gene            complement(2454..3266)
                     /locus_tag="VN496_11945"
     CDS             complement(2454..3266)
                     /locus_tag="VN496_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF17713.1"
                     /translation="MTHWKPVYAVLEREVVRMFRQRTRLVSAMVRPLIWLFVIGAGFG
                     TVLQEAGGLSYQHFLVPGVLGMTMLFGAMLAALTMVYDKESGVMRMLVMAPFAHYWIV
                     IAKMLSAALAALLQAVLLVALLAMLRYVDSGTRFALLALALLMTSAACAGIGMLVAAF
                     TRTLDNFAAIMNFVIFPVFFLSGSLYPVSRLPAVLKSVALMNPYTYGVDLLKHSVLGA
                     IPSGMGADFAISTDVVVLAGFTGAALLIASWRFSRESAYEPMIRILSGKRDA"
     gene            complement(3263..4198)
                     /locus_tag="VN496_11950"
     CDS             complement(3263..4198)
                     /locus_tag="VN496_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF17714.1"
                     /translation="MNPAPATSLAVNARGLVKRYGDVLAVDGLDLQIPAGQFFGLLGP
                     NGSGKTSTIHMLATLIRPSGGSASVFEHDVMEDAVKTRASIGLVFQESALDRTLSVGE
                     NLRFAGLLYNLPVHIIEERSAGLLELFGLADKRHRPVGSLSGGMRRALDIVRGVIHHP
                     RILFLDEPTIGLDLPNRRKIWRFIEDLRARTGMTVLLTTHYLEEADTCDKVAFIRNGK
                     LVSSGTPKEMIARLGRYIVEIESSELETLAKQLAPSLGLCLRDGNVAQFRYADEDLTP
                     IASLQSQYGTHIRALRWRRPNLNDVFLWIAEGKAG"
     gene            complement(4213..5361)
                     /gene="pqqE"
                     /locus_tag="VN496_11955"
     CDS             complement(4213..5361)
                     /gene="pqqE"
                     /locus_tag="VN496_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HXF17715.1"
                     /translation="MEKAGSVPSKPGNPLWLLAEVTYKCPLHCVFCYNPVDYARYGAE
                     LSTEDWLRVLRQGRELGATQLGFSGGEPLMRDDLEIMVSEARKLGYYSNLITSGIGLN
                     EKRIAAFKEGGLDHIQLSFQDSTREMNDFLSSTKTFELKQKVAKLVKKYDYPMVLNCV
                     LHRHNIDHVQQILEMAEAMDAEYVELANTQYYGWALLNRDQLLPTRDQLKRAEEVTNK
                     FRERVGNKMRIFFVVPDYYEERPKACMNGWGALFLTVTADGLALPCHEARMLPGLNFP
                     NVRDHELGWIWYESPGFNAYRGDSWMKEPCRSCPDKVKDFGGCRCQAYMLTGDATNTD
                     PVCGLSPLHNVVTEAVDKAQSPTAPRVDVKPIVFRDDKNSRALSKTPA"
     gene            complement(5333..5614)
                     /gene="pqqD"
                     /locus_tag="VN496_11960"
     CDS             complement(5333..5614)
                     /gene="pqqD"
                     /locus_tag="VN496_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HXF17716.1"
                     /translation="MADAAFTEDTVFELQRHFRFQWEPAQQGFVLLYPEGMVKLPGSS
                     GEIMKRIDGKLSVRGIVEDLQTAFPGTDLRGDVIGFLEHVYGKGWIRPK"
     gene            complement(5607..6326)
                     /gene="pqqC"
                     /locus_tag="VN496_11965"
     CDS             complement(5607..6326)
                     /gene="pqqC"
                     /locus_tag="VN496_11965"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HXF17717.1"
                     /translation="MGAADNLPWTKPEFENELRAKGKRYHIYHPFHIAMNSGKCTREQ
                     VQGWVYNRFYYQISIPIKDAAILANMPDREERRKWIMRIIDHDGMEGQEGGIEAWLRL
                     AEACGLKREDVTSLQYVLPGVRFAVDAYVNFARRAPWQEAACSSLTELFAPEIHQKRL
                     DSWPQHYPWIEPEGYAYFRKRLSEARRDVEHGLQVTLEYFKTRAEQQRALGILQFKLD
                     VLWSMLDAMQINYGIGAAAHG"
     gene            complement(6338..7258)
                     /gene="pqqB"
                     /locus_tag="VN496_11970"
     CDS             complement(6338..7258)
                     /gene="pqqB"
                     /locus_tag="VN496_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HXF17718.1"
                     /translation="MSIRVRVLGASAGGGFPQWNCNCRNCDGVRKGTLRATARTQSSI
                     AISGDGMNWILFNASPDLLTQYKGFAELQPARSIRDTAIRSVVLMDAQIDHTTGLLML
                     REGRPLEIYCTDMVRDDLSTGNPLFKILEHYCGVNWHEIPIRQGSSFGIIGAEGLAFT
                     AVASKSNAPPYSPHRDKPIAGDNIGMRIVDPQSGKVLFYAPGLGEIESHLKPFLSEAD
                     VLMVDGTFWTDDEMIRLGLSRKHARDIGHLPQSGTGGMIEVLKPLKASRKILIHINNT
                     NPILDEDSPERRQLTAAGIEVAYDGLDLTL"
     gene            complement(7349..7420)
                     /gene="pqqA"
                     /locus_tag="VN496_11975"
     CDS             complement(7349..7420)
                     /gene="pqqA"
                     /locus_tag="VN496_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HXF17719.1"
                     /translation="MWSKPEYTEMRFGFEVTMYIANR"
     gene            7638..>8462
                     /locus_tag="VN496_11980"
     CDS             7638..>8462
                     /locus_tag="VN496_11980"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXF17720.1"
                     /translation="MSDAIFDSGVYYSSPTDWYRERPFKVAFAVSVVVHAVLIASLPG
                     LRSVPVDSPPVLQVEIAQTLPPEIPLTEQAPPVPPRQVEPKPELQLRRPERPREPPLQ
                     RAYPERPVAPIADIVEAPRPEVTPEFVRPKVEPPPAPVEQAKPEPQIEPPPAPIARIE
                     LPPQPAVERATDPVPDFKSNKVPDARPVPREPHVVAPQTKAPDASRERASTDLYRQLI
                     SAQIRKYQDYPPIAKRRRWEGTTIVELHFTAEGKVGDILVSEKSGHDVLDEAAVRMV"
BASE COUNT         1907 a         2371 c         2346 g         1838 t
ORIGIN      
        1 aaacgagggc aagcacgatc cagcgtgcgc gtgtcatgaa gcactatctg caggctgttg
       61 cgccggcttg cacggaaaaa atttcgcagc agactgacgc gatgcacgga gttgtatccg
      121 cttcccagcg tttgatcgaa gatccgaatg gttaccgaag cttttgcgag aacggtatcg
      181 attgcagatt cgtactcggc attaccgaaa agtcgcgtgt gctcaggatt tgaacgctgt
      241 tgcatgggaa tcaatcgtcc ctgcctggtt gggacagcac aatataaccg gcgtgatacc
      301 agcggtaaag caagtccatt gccgcgccgt ctatcggaac ttgcagtggc gcaagccggc
      361 gcgtatcagc cagccgccgc aacacaggta ctgcctttac tgttgtatac gattcaccgt
      421 tcatgaaatg ggttgcgcgg ctgtacagca tcgacgaagg caatgccaat tccacgccac
      481 gttttttggc ggccatggaa aaggcagcct cagataacct ttggtgcggt ggcttgaaca
      541 caacgcgcgc ttttggttcg gtcaggtaaa tgcccagaaa ttcggccacg tcgcgctgtt
      601 gccaccgcgt gtttgccagc aggcgctcca ctttggcgac catgctggac ccgagccgtg
      661 ccggatgtgc ctgtggcaac agatccggat cgctgtagcg gccggtggct tgtgctttat
      721 cctcgaggtg aacgaggaac tgggatgtca gttcctggtg cgacggcgca cgaaaaccga
      781 tcgaatacgt aaagcagcaa tcgaccgcga tgccctcgtg agcacaggcc ggtggcagat
      841 aaagcatatc gcccgcggag acaaggcatt caccttcggg taaaaaccgc ttcagaagtt
      901 tcagtggcgc gccttcgacc aaagccatgt cgcgctggcc gctgactctc cagcggcgct
      961 ggcccggtcc ttgcaacaaa aaaacatcgt aagagtcgaa atgtggcccc acgccgcctc
     1021 ctggcgcagc gtaactcacc atcacatcat cgagccggct gtatggaatg aaatcaaaat
     1081 tggatagcaa cttgcgcgca gcgggtaaca tgctctccag gccttggacg agcaatgtcc
     1141 aattgcgtgc cgggaggcgt gagaatcgct tgcgatggaa gggaccgtgc tcgacggtcc
     1201 agcgagtgcc atggcgtacg accaaccggg attcgcagtc ttggcgggat gcgaactcca
     1261 ccagtgcgcc gaaatcctgg attccgtcaa aatgtggaat ggcgttgcgc acgagcaggg
     1321 gctttttttg ccaatgtcga cgcagaaacg cagcagcgga taggccgccc aaaagctgtt
     1381 tgttcacgtt gttcattgta tcggcgtgcg ctatgcgatg ccctattcgc gtttttgaaa
     1441 tcggttagaa tccaccccgg atgcgatcgg agttgtacat gctcaaagta ggtgaagtcg
     1501 ctcccacgtt cgaccttccc gacggcgata tgaatgtggt gagcttgagt gcctatctcg
     1561 gcgtcaataa cgtggtgctg tatttttatc ccaaggacga tacgccgggg tgcacgaggg
     1621 aggctatcga tttcagcgac cgggaggatg attttctgga cctggatacc gtcgtgctcg
     1681 gtgtgagcat ggacgactgc atgagccatg gtgcctttcg cgataagcac ggccttgccg
     1741 tgaaattgct cgcggacacg gaaggcgagg tctgcgaaaa atacggggta cttcaggaaa
     1801 agaaagtgga gggcaaaggt gagcggcgcg gcatcctgcg ttcgacattc gtcatcgata
     1861 agagcggcgc attgcgcaac gtgctttatg gcgtcaatgc gcgcggacac gcagccgatg
     1921 ttttaaacct cgtaaaggag ttgaagtgag catgcagatc agcaaagaca cggtggtgtc
     1981 tctgaactat gaattgagcg atctcaaagg gaaggtgatc gaaaaaaccc aggctcccat
     2041 cacctatttg catggtggat acgacggtat attccccttg gtcgaggtag cgctccaggg
     2101 caaaagtatc ggtgataaat gcaacgtaac gctgaagccg gacgacgctt ttggcgaata
     2161 cgaaaaagat atggtccgaa ccgagccgaa aagtcttttc cccgagaata tttctgtggg
     2221 catgcaattt gaaggggcga ttgaaggctc agatgattac accgtctaca ccgtcaccga
     2281 gatccagggt gagaagatcg tggtcgacgg caatcacccg ttggccgggc aaacgctcgt
     2341 gttttccggc attgttacgg gagttcgtcc ggcaacgcac gaagaactgc accatggcca
     2401 tgtccatggc ccggatggtc accaccacta ggcattagcg cttcagaaca gcgtcaggcg
     2461 tcgcgcttgc cggaaagaat gcggatcatt ggttcatagg cgctttcgcg cgagaaacgc
     2521 cagctcgcaa ttaacagtgc ggcgcccgtg aatcccgcca gcacgacaac atcagtcgat
     2581 atcgcaaaat cggctcccat cccggacgga attgcgccta gaacggaatg cttcagcagg
     2641 tcgacgccat aagtataagg attcatcagg gcaactgatt tcagcacagc gggaagacgg
     2701 cttaccggat acagcgaacc cgataaaaag aataccggga agatcacgaa attcatgatg
     2761 gccgcaaaat tgtccagtgt gcgggtaaat gctgccacca gcatgccgat gccggcgcac
     2821 gcagctgaag tcatcagtaa tgcaagcgcc agcaaggcga agcgggtgcc cgaatccacg
     2881 taccgcagca tcgcgagcaa cgccactagc aatactgcct gaagcaaagc agccagcgct
     2941 gcggacagca tctttgcgat aacgatccag taatgcgcaa agggcgccat gaccagcatg
     3001 cgcatgacgc cggattcctt gtcgtacacc atggtgagcg ccgcaagcat ggcaccaaac
     3061 agcatcgtca tacccagtac gcccggcacc aggaaatgct ggtagctcaa accgcccgct
     3121 tcctgcaaga ccgtaccaaa gccagctcca atcacaaaca gccagatcag cggacgaacc
     3181 atcgcggaca ccagtcgcgt gcgctggcgg aacatgcgta ctacctcacg ttcgagtacc
     3241 gcgtagaccg gcttccagtg tgtcatcctg ctttgccctc cgcgatccac aggaacacat
     3301 cgttcagatt gggtcgtctc cagcgcaatg cgcggatgtg agtgccatac tgggattgca
     3361 agctagcgat cggagttaga tcttcgtctg cgtaccgaaa ttgggctaca ttcccgtcgc
     3421 gcaggcaaag tccgaggctc ggcgccagtt gcttcgcgag ggtttcgagc tccgaacttt
     3481 cgatttcaac gatgtaacgg cccaaccgag cgatcatttc tttcggggtg ccgctactga
     3541 ccagtttccc gtttctgatg aatgctactt tatcgcaagt gtccgcttcc tcaaggtaat
     3601 gggtcgtcag caggacagtc atgccggtcc gcgcgcgcag atcttcgatg aagcgccata
     3661 tcttgcggcg attcggtaaa tcaaggccaa tcgtgggttc gtccaggaat aaaattctag
     3721 gatgatgaat gacgccgcgc acgatatcga gtgcccggcg catgccgcct gacaggcttc
     3781 ccaccggccg gtgtcgcttg tcggcaagtc cgaataattc cagcagaccg gcggaacgct
     3841 cttctattat atggaccggc aggttgtaga gcaggccggc aaagcgcaga ttctcgccaa
     3901 ccgacagggt tcgatcgagc gccgattcct ggaagacgag cccgattgat gcccgtgtct
     3961 tcaccgcatc ctccatgaca tcgtgttcga acacgctcgc gctgccgccg ctggggcgaa
     4021 tcagcgtggc gagcatgtgg atggttgacg tttttcccga gccgttgggg ccgagcaagc
     4081 cgaaaaactg ccctgcggga atttgaagat cgaggccgtc tacggcgagt acatcgccgt
     4141 aacgcttgac aaggccgcgg gcgtttacgg ccagcgacgt agcgggcgcc gggttcatgg
     4201 ccgcaaggga cgctaagccg gtgtcttgct caaggcgcga gaattcttgt cgtcgcggaa
     4261 gacgatcggt ttgacatcca ctcttggcgc cgttggtgat tgcgccttgt cgacagcctc
     4321 ggtgaccaca ttgtgcagtg gagacaagcc gcataccgga tcggtattcg ttgcatcacc
     4381 ggtcagcatg taagcctgac aacggcaccc accgaagtcc ttcaccttgt ccggacaaga
     4441 ccggcaaggc tccttcatcc acgagtcacc ccgataggcg ttgaatcccg gagactcgta
     4501 ccagatccag cctaattcgt gatcacgcac atttggaaaa ttcaaccctg gaagcatgcg
     4561 cgcctcatgg cagggcagcg caaggccgtc ggcagtaacg gtcagaaata gtgcacccca
     4621 gccgttcatg caggctttgg ggcgttcctc atagtagtcc ggaacgacga agaaaatgcg
     4681 catcttgttg ccgactcgtt cacggaattt gttagtcact tcttcggcac gtttaagctg
     4741 gtcgcgtgtg ggcagcaact ggtcacggtt gagcagggcc cagccatagt attgggtgtt
     4801 cgcgagctcc acgtactcgg catccatcgc ttcagccatt tccagtattt gctgaacgtg
     4861 gtcgatattg tggcggtgca agacacagtt cagcaccatc ggatagtcgt atttcttgac
     4921 taatttcgcg actttttgtt tgagctcgaa ggtcttggtg cttgacagga aatcgttcat
     4981 ttcacgggtc gaatcctgga acgacagctg aatatggtcc agcccgcctt ctttgaaggc
     5041 cgcaatgcgc ttctcattga gtccaatccc ggaagtaatc agatttgagt aatagcccaa
     5101 tttgcgagcc tcggaaacca tgatctccaa atcatcgcgc atcagcggct cgccgccgga
     5161 gaaaccaagc tgcgtggcgc cgagttcccg gccctggcgt aacacgcgca accagtcttc
     5221 ggttgacaac tctgcgccgt agcgcgcgta atcaaccggg ttgtagcaga acacgcagtg
     5281 cagcgggcac ttgtaggtta cttcagccag cagccagagc ggattacccg gcttacttgg
     5341 gacggatcca gccttttcca tagacatgct ccaggaatcc gatgacgtcc ccgcgcaggt
     5401 ccgtaccggg aaatgcggtt tgcaaatctt cgacaatgcc gcgcacgctg agtttgccgt
     5461 cgattcgctt cataatctca ccagaactgc ccggcagctt gaccataccc tccgggtaca
     5521 gcagcacgaa accctgttgg gcaggttccc actgaaagcg aaagtgccgc tgcaattcga
     5581 agaccgtgtc ttcggtgaat gctgcgtcag ccatgtgccg ctgcgccgat gccatagttg
     5641 atttgcatgg cgtcgagcat gctccacagt acgtcaagct tgaactgcag gatgcccagc
     5701 gcacgttgct gctcggcccg ggtcttgaag tactcgagcg tgacttgcaa cccgtgttcc
     5761 acgtcgcgac gagcctcgga caaacgttta cggaagtagg cgtagccttc cggttctatc
     5821 cacggatagt gctgtggcca gctgtccaag cgtttctggt gaatttccgg cgcgaatagc
     5881 tcggtaagag aagagcaggc tgcctcttgc cacggagcgc gtcgagcgaa attgacatat
     5941 gcatcgacag cgaagcgcac accgggcaac acatattgga gcgaagtgac atcctctcgc
     6001 ttcaagccgc atgcctcggc tagtctcagc caagcctcga ttccgccttc ctggccctcc
     6061 attccatcgt ggtcgataat gcgcatgatc catttgcggc gctcttcacg gtctggcatg
     6121 ttggccagaa tggcggcgtc cttgatcgga atgctgatct gataatagaa acggttgtaa
     6181 acccaccctt gcacctgctc cctggtgcat ttgccgctgt tcatggcaat gtggaacgga
     6241 tggtagatgt ggtagcgctt gcccttggca cgcaattcat tttcgaattc tggcttggtc
     6301 cacggtaaat tgtctgcggc acccatttga atctccgtca aagcgttaaa tccaggccgt
     6361 cgtatgcgac ttcgatgccc gccgcggtta gctgcctgcg ttcgggcgaa tcttcgtcga
     6421 gaatggggtt ggtgttattg atatggatta agatcttgcg cgatgctttc agtggtttga
     6481 gtacttctat catgccgcca gttcccgact ggggcaaatg gccgatatcg cgagcgtgct
     6541 tcctggacaa acccagtcga atcatttcgt cgtccgtcca aaatgtgcca tcgaccataa
     6601 gaacgtccgc ctcgcttaaa aaaggtttca gatgggattc gatttccccg aggccgggag
     6661 catagaacag tacttttccg gactggggat ccacaatacg catgccaatg ttgtctcccg
     6721 caattggctt gtcacggtgc ggtgaataag gcggcgcatt gcttttcgat gcgacggcgg
     6781 tgaaggcgag tccctctgcg ccgattattc cgaaactgct gccttgcctg atcgggattt
     6841 cgtgccaatt gactccgcaa taatgctcaa gaattttgaa aagcggattt ccggtgctga
     6901 gatcatcgcg aaccatgtcc gtgcaataga tctccagggg acgcccttca cgcaacatca
     6961 gcaaaccagt ggtatggtcg atttgcgcat ccatgagcac gacggaccgg attgccgtat
     7021 cccggattga ccttgcgggt tgcaattctg caaatccttt gtattgcgtc agcaggtcgg
     7081 gtgaagcatt gaacaaaatc cagttcattc catcgccgct gattgcaata gaagattgtg
     7141 ttctcgctgt cgcgcgtaac gttcctttcc ggacaccgtc gcagttccgg caattgcaat
     7201 tccattgcgg gaatccgccc cccgcggatg cacccaggac tcttactcgt atggacatcg
     7261 gttcggccga tcgtttgggt gttttacttt cgccagaaaa gacaaaagcc ccgtaatggg
     7321 gcttttgtcc aagcccagcg ctgatgaatt agcggttggc gatgtacatc gtcacttcaa
     7381 agccgaaacg catttcggtg tactcaggct tgctccacat agttttctcc aaagagttag
     7441 ttagattgac attgttgctc gaatcattga acccggacgc cgcaatgaca tatcgggaac
     7501 cagtccaacg agtccaaagc attttccgga atgtttaccg ctatcaccct agaaaaatca
     7561 ccaattcgat atcatgattt tcattagttt gacgggcaaa ctttacttac ctcaacgggt
     7621 tgcactaacc ttcagccatg tccgatgcca tcttcgacag cggcgtttat tattcatcgc
     7681 caaccgattg gtaccgcgaa cgcccattca aagtggcctt tgcagtgtcg gtggtcgttc
     7741 atgccgttct gattgcctct ttgcctggtc tgcgttccgt gcccgtcgac tccccgccgg
     7801 tgttgcaagt cgaaatcgcg cagacactac cgcccgaaat tccccttact gaacaagcgc
     7861 cgcccgttcc acccagacag gtggagccca agcccgaact ccaactgcgg cgcccggaac
     7921 ggccgcgcga gccgccacta cagagggcat accctgaacg cccggtcgct cctatcgccg
     7981 atatcgttga agcgcctcgt cccgaagtta ctccggagtt tgtgcgaccg aaagtcgaac
     8041 cgccacccgc gccggttgag caagcaaagc ccgaaccgca aatcgagccc ccgcctgcgc
     8101 cgatcgctcg aatcgagctg ccgccccagc cggccgttga gcgcgcaacg gacccggtcc
     8161 cggatttcaa gagcaataag gttccggatg ccagaccggt gcctcgtgaa cctcatgtcg
     8221 tagcgccaca gacgaaggcg ccggacgcca gtcgggaacg cgcctccacc gacctttaca
     8281 gacaattgat ctctgcgcag atccgcaaat atcaggatta tccgcccatc gcgaaacgcc
     8341 gacggtggga ggggacaaca attgtcgagt tgcacttcac tgcagaaggc aaggtgggcg
     8401 atatcctcgt ttccgaaaag agcggacatg acgttctcga tgaagcagct gtaaggatgg
     8461 tc
//
LOCUS       DATMZQ010000371         4846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1331756, whole genome shotgun sequence.
ACCESSION   DATMZQ010000371 DATMZQ010000000
VERSION     DATMZQ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331756"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="VN496_11985"
     CDS             <1..1794
                     /locus_tag="VN496_11985"
                     /inference="COORDINATES: protein motif:HMM:NF010178.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HXF17721.1"
                     /translation="TKSFFGTYARSLRLLSKERRAVALLAVTNLVAATLFFFEPILFG
                     RLIDVLAGAATHSSAEIWHASLMTLGAWAAVGLSGLLANFVLALSADRLAHRKRLALT
                     VSYFEHVLALSFEYHSDKHSARLLRIMLRATTYLFGFWLAFFREHLGTLITLIVLLPL
                     SMLLNWRLGLLLGLFMAAFTIFTAFSIRRMETAQAAVEAADSELTARVGDAFGNVRLI
                     QSYVFRAAETMALAQLTRRILGAQYPILAYWAGINVLTRAASTITIIGIFAMGTWLVF
                     KAQSSVGQIVTFMGFAALLIGRLEQVGRFISQMFLEMPSVAAFFDVMDTPPVIRDRPG
                     AIELHRVRGQVQFENVSFCYVPDRPAISEISFEAAPGSMVALVGATGAGKTTTASLLT
                     RLHDPGVGSIRIDGIDIRDVTVDSLRHNIGIVFQESALLHRSIAENIRIGRPTASDEE
                     VLQAARSAEAHEFIMRQPLGYDTTVGERGASLSGGERQRIAIARALLKDPPILILDEA
                     TSALDSITEASVQRALQVLMRGRTTFVIAHRLSTVRSADFILVFSRGKIVERGTHQIL
                     IKHNGPYAQLVRAQMEAGASPITTAQGAPGE"
     gene            complement(1847..2050)
                     /locus_tag="VN496_11990"
     CDS             complement(1847..2050)
                     /locus_tag="VN496_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXF17722.1"
                     /translation="MASGTVKWFNDAKGFGFITPDDGGKDLFAHFSAIQGNGFKSLKE
                     NQKVTFDKTTGPKGDQASNIRPA"
     gene            2396..2656
                     /locus_tag="VN496_11995"
     CDS             2396..2656
                     /locus_tag="VN496_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HXF17723.1"
                     /translation="MPAACRHLNQIRDVQPSANGCEDCLKIGDTWVHLRICLECGHVG
                     CCDSSKNKHATRHFQKTDHPIIQSFEPGESWRWCYVDQVFME"
     gene            complement(2675..3640)
                     /locus_tag="VN496_12000"
     CDS             complement(2675..3640)
                     /locus_tag="VN496_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17724.1"
                     /translation="MKGTLLFVSAFALLLVGHAVRANPDDALPVWAYPVNPPGLKPAP
                     DDGSLKRVPNSNAAYTMTQIRDLFSPPDWHPSSHPAMPDVVGRGSKPGQFPCGFCHLP
                     NGLGRPENSSLAGLSAEYIAQQVADFRSGARRSSEPASLPVNLMVAVAKAVSDEDAKI
                     AAAYFAGLKPVPWIRVVETDTVPKTKVGGWMLIADGSGAMEPIGQRIIEMPEDLERTE
                     LRDSASGFIAYVPVGSLQRGEALTASGNGKTTPCAICHGADLKGLGPVPGLAGRSPSY
                     IVRQLYDIKHGTRDGQWTALMKSVVSGLSQEDMVSIAAYTASLKP"
     gene            3897..>4846
                     /locus_tag="VN496_12005"
     CDS             3897..>4846
                     /locus_tag="VN496_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF17725.1"
                     /translation="MRYRLLGRTGLLVSEICLGTMTYGGKGRWQPIGQLALGDVEGQI
                     KTAVEAGMNFIDTADVYSEGDSESLVGQALKNLKLPREELVIATKVRIRMGPGPNQVG
                     LSRGHIMDGVNASLRRLQLDHIDLYQIHGQDVATPMEETLRALEDLIRSGKVRYIGLS
                     NHAAWQIMKAVSISQHRGWNRFESVQAYYSLAGRDLEREIVPLALDQGLGILVWSPLA
                     GGLLSGKFAENDKGPQGARRTNFDFPPIDRPRAFRCVEAMRPIAQTHGVSVARIALAW
                     LLHQPAVASVIVGAKTREQLADNIAATDVRLSEAELKSLD"
BASE COUNT         1029 a         1395 c         1361 g         1061 t
ORIGIN      
        1 acgaaatctt tttttggaac ttatgcgcgc tctttgcggc tgttatcaaa agagcggcgg
       61 gctgtcgcac tgctcgccgt tactaacctg gtggccgcaa cgctgttctt cttcgaaccg
      121 atactgtttg gacggctgat cgacgtgctg gcgggtgcag ccacgcattc cagcgcagaa
      181 atctggcatg ccagcctgat gaccctcggc gcatgggcgg cagtgggcct cagtggcctg
      241 ctcgccaatt tcgtgctggc cctgagcgcc gaccggctcg cgcaccgtaa acgtctcgca
      301 ttgactgtaa gttatttcga gcatgtgctc gctttatcat tcgagtatca ctcggacaag
      361 cactccgcac gcctgttgcg catcatgctg cgggccacaa catatctgtt tggtttttgg
      421 ctcgcgtttt ttcgcgagca cctgggtacg ctgatcacac tgatcgtatt gcttccgcta
      481 tcgatgttac tgaattggcg gctcggcctg ctgctgggac tcttcatggc agcattcact
      541 atattcactg cgtttagcat ccgcaggatg gaaacggcgc aagccgctgt tgaagcggcg
      601 gactccgaac tcaccgcccg ggtcggggat gcttttggaa acgtgcggtt aatccaaagc
      661 tatgttttcc gtgccgccga gacaatggcg ttggcgcaac tgacccggcg catcctcggc
      721 gcgcagtacc cgattctggc ttattgggca ggcatcaatg tgctgacgcg cgccgcatcg
      781 accatcacca taatcggcat ttttgcgatg ggcacctggc tggtgttcaa agcgcaaagc
      841 agcgtgggtc agattgtcac cttcatggga ttcgctgcat tactgatagg acggcttgag
      901 caagtcgggc gtttcatcag ccagatgttc ctggaaatgc ccagcgtcgc cgcgttcttc
      961 gacgtcatgg atacgccacc ggtaatccgg gatcggccag gggccatcga gcttcatcgt
     1021 gtgcgaggcc aagtccaatt cgaaaatgtc tctttctgtt atgtacccga tcgcccggca
     1081 atcagtgaga tcagttttga agctgcaccc ggcagcatgg tagcgctcgt gggcgccacg
     1141 ggcgccggaa aaacgaccac tgcttccctg ttgacccgtt tgcatgatcc aggcgttggc
     1201 tccatccgca tcgatggaat cgacattcgc gacgtgaccg tggattcctt gcgccacaac
     1261 atcggcattg tttttcagga aagtgctttg ctgcacagat cgatcgcgga aaacatcagg
     1321 atcgggcgac caactgcgag cgacgaagaa gtgctgcagg cagcacgatc cgcagaagct
     1381 catgagttca tcatgcgaca gccgcttggc tacgatacga cggtcgggga acgcggcgct
     1441 tcactcagcg gcggagagcg ccagcgcatt gccatcgcgc gcgcactgct gaaggacccg
     1501 ccgatcctga ttctcgatga ggcaaccagc gcgctggatt ccattaccga agccagcgtg
     1561 cagcgggcac tccaggtcct gatgcgtggg cgaaccacgt tcgtcatcgc acaccgtctt
     1621 tcgaccgtcc gcagcgccga tttcattctg gttttcagtc gtggaaaaat cgtcgaaagg
     1681 ggcacgcatc agatactgat aaagcacaac gggccttacg ctcaactggt acgcgcgcag
     1741 atggaagccg gtgccagtcc aatcaccacg gcgcaaggcg caccgggcga atagccaaaa
     1801 aaaagcccgg gaatccccgg gctttttgca tgcctgctcc gaccgcttac gcggggcgga
     1861 tattagatgc ctgatccccc ttgggcccgg tggtcttgtc gaacgtgacc ttttgatttt
     1921 ccttgagcga tttgaaaccg ttgccctgga tcgcggagaa atgcgcgaac aaatccttac
     1981 cgccatcgtc cggggtaatg aacccaaaac cttttgcgtc gttaaaccac ttgaccgtac
     2041 ctgatgccat cttcgttcgt ccttgaaaaa aattagtaat aagtaattcg ccgggaggcg
     2101 gcagaccaag aggagaaatt aaggagatct gaggagtacc gttggaacgg gctgcgacgg
     2161 accagcaatt cagcgcttgg gggtttctgc gccttcctta tacgcctttt tccaatgtcc
     2221 agcaaagggc ggattggacc ggcaggacgc ttgttttgta tgacatatac ggcacatttt
     2281 atgaccctgg tctgcggcgt tttcgtctta ccgacacgaa caaccccggg agccggccac
     2341 cgcaaaaaac gaatcctgcg tactattgga gaccttcaac cttctaacca caccaatgcc
     2401 agccgcttgc cgccatctca atcaaattcg tgacgtacag ccctccgcca atggttgcga
     2461 ggattgcctg aaaattggcg atacatgggt tcacctgcgc atctgcctcg aatgcggtca
     2521 cgtgggttgt tgcgattcat ccaagaacaa acacgcgacc agacatttcc agaagacgga
     2581 tcatccgatc atccagtctt ttgaacccgg agaatcgtgg cgctggtgct atgtggacca
     2641 ggtcttcatg gaatagcgcg gtgaacttgc ttggtcacgg tttgagcgac gcggtatacg
     2701 ccgcgatcga caccatatct tcctggctca gtccactgac gacagacttc atcaacgccg
     2761 tccattgccc gtcacgcgtg ccgtgcttga tatcgtacaa ctggcgaacg atatagcttg
     2821 gcgagcgccc ggcaagtccg ggcaccgggc caagaccttt caaatccgcg ccgtggcaaa
     2881 tggcgcacgg cgtggtcttg ccatttccgc ttgccgtcaa ggcttcacct ctctgcagac
     2941 tgccgactgg cacgtaagca atgaagcccg atgcagagtc gcgcaattcc gtgcgctcca
     3001 ggtcttccgg catttcgatg atgcgctgtc cgataggctc catggcgcca ctaccgtcgg
     3061 cgatcaacat ccagccgcca actttggttt tgggcaccgt gtcagtctca acgacccgga
     3121 tccagggcac cggtttcagt ccggcaaaat atgcagcggc aatttttgcg tcttcatcgc
     3181 tgacggcttt cgccacggca accatcaaat tgaccggcag gctcgccggc tctgaacttc
     3241 tcctcgcacc gctgcgaaaa tcagccactt gctgggcaat atattccgcg gacaacccgg
     3301 caaggcttga attttccggt cggccgagcc cgttcggcag gtgacagaaa ccacaaggga
     3361 actggcccgg cttgctaccg cgtccaacga catccggcat agccgggtgc gaactggggt
     3421 gccaatccgg cggactgaac agatcccgta tctgcgtcat cgtataagcc gcgttactat
     3481 ttggcacccg cttgagtgaa ccgtcgtcgg gcgccggctt gagtcccggt gggttgaccg
     3541 gataagccca aacgggtagc gcatcgtccg gattggctcg gaccgcgtga ccgaccagca
     3601 gcaaagcgaa tgcggaaacg aacagcaaag tgcccttcat ggctccctcc aattattttt
     3661 atgaccttct gccttcgatg cattataagc acacccgtgc aagcgcgaga ggcgacacaa
     3721 attaacgcca tgaccaggcg ccgcgtctag tcgttaccgg ccttgccgat caggcgggta
     3781 gcgaatccga cttttggtct attaccccgc atgcgcatta aattgttatc ttgtaaaccc
     3841 gttatggcac gctgttacgc gaagccactc gccaactaaa gatttccgga ggattcatgc
     3901 gctaccgact attgggccgt accggccttc tggtttcaga aatttgcctc ggcaccatga
     3961 cgtatggcgg aaaaggacgc tggcagccga tcggacaact tgcccttggc gacgtcgaag
     4021 gccagatcaa aaccgctgtc gaggccggca tgaatttcat cgataccgcg gacgtgtatt
     4081 cggaaggaga ctcggaaagt ctcgtgggcc aggcgttgaa gaacctgaag ctgccacgcg
     4141 aagagctcgt tatcgcgacc aaagtgcgta tccgcatggg accggggcca aaccaggtcg
     4201 gattgtcgcg cggacatatc atggacggag tcaatgcgag tctgcggcgg ctgcagctgg
     4261 atcatatcga tctctatcag attcacggtc aggatgtggc cacgccgatg gaggaaacac
     4321 tgcgggccct tgaagacctg atccgttcag gcaaggttcg ctatatcggg ttgagcaatc
     4381 atgctgcctg gcaaataatg aaagcggttt ccatttcgca gcatcgcggc tggaatcgtt
     4441 tcgaaagcgt gcaagcgtat tactcgcttg ccggtcggga tctcgaacgc gaaatcgtgc
     4501 cgctggcgct ggatcagggc ctgggcattc tggtatggag tccgttggcg ggaggcctgc
     4561 tgtctgggaa attcgccgaa aacgacaagg gacctcaagg cgcaagacgc accaatttcg
     4621 attttccgcc aatcgacagg cctcgcgcgt ttcgttgcgt tgaggcgatg cgcccgattg
     4681 cgcagactca tggcgtctcc gttgcacgta tcgcgctggc ttggttgctg catcagccgg
     4741 cggtagcaag cgtgatcgtt ggagcaaaga ccagggaaca gctcgcagac aacattgcgg
     4801 ctaccgacgt gcgcctgtcc gaagcagagt tgaaatccct cgacga
//
LOCUS       DATMZQ010000372         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2711957, whole genome shotgun sequence.
ACCESSION   DATMZQ010000372 DATMZQ010000000
VERSION     DATMZQ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711957"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..460
                     /locus_tag="VN496_12010"
     CDS             110..460
                     /locus_tag="VN496_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17726.1"
                     /translation="MHEIKLADFRNRRVAIWVQNRWLVYVCLVVCLLLLTLAGALNVP
                     AQLAWTAGTLACGLIAFTVTRVVRGLHVLYRCPICGVLPYRTLNEYKCGGLGPARANF
                     MSPRSCPQCGTQIR"
     gene            511..2193
                     /locus_tag="VN496_12015"
     CDS             511..2193
                     /locus_tag="VN496_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXF17727.1"
                     /translation="MDRSPLTPTLSGHTAFMKLLAQEGVTHLFGNPGTTELAIMEAMS
                     QQSDIAYVLGLQESVVVGMADGYARASNQLAACNVHVAPGLGNAMGALYNAKFYGSPV
                     LITAGQQEQGHGLMEPMLYDPLVPIAQPMVKWAVDVARVQDLPRIVHRAVKLALTPPT
                     GPVFISLPGDILDAQAPLDLGYPTRVDAEVRPSDAALARLADRLLAARNPVLIAGHEL
                     ATRNALQEAAQLAEILGAAVWQQTVPYAAHFLSEHPAFVGQLTRSQTQVRAWLQPHDL
                     VVCLGADVLRMSVHNPIDALPDGMPIVQISERDWELGKNYPTDIAINANVKQSLLALN
                     SVMRARMTAAQKSAAAARLAEIAKNNWSVKRARACEDAAKLESTRPMDPKVLMMRIAE
                     ALPRNVVVVEEGLTSTFPLLNFLPLRDHQGFYGLASGGIGFAMAGAVGISIALPDRPV
                     VAIVGDGSAMYTIQALWTAAHLKLPITYVIPNNRSYRILKERLVSFRKTDKFVGMDMD
                     DPAIDFVSLAQSMGVKAQRITDQQDVGPALRECISSGGPRLLDVSVANGFGR"
     gene            complement(2269..>2504)
                     /locus_tag="VN496_12020"
     CDS             complement(2269..>2504)
                     /locus_tag="VN496_12020"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HXF17728.1"
                     /translation="LLVNGFGGTPLMELYILYHAAHGFLTKASVRVVRSLVGNYVTSL
                     DMAGGSVTVTMLDSELKRLWDAPVHTAALRWGV"
BASE COUNT          493 a          756 c          731 g          524 t
ORIGIN      
        1 gtcacggcaa accttcgcca atacggtgct gtgggcaaag gcgccccaag cttgagcatc
       61 gacaaccaac gccgcacaat gccatctcgt cgcagtgtag ggtttcggca tgcacgaaat
      121 caagctagcg gattttagga atcgccgcgt cgccatctgg gttcaaaacc gctggctggt
      181 atacgtttgc ctggtcgtct gcctgttgct tttgactctc gccggtgctt tgaacgtgcc
      241 ggcgcaattg gcatggaccg cgggtacgct ggcctgtggt cttatcgcat ttaccgttac
      301 gcgcgttgtt cgcgggctgc acgtgctata tcgctgtccg atttgcgggg tgctgcccta
      361 tcgcacgctc aacgaataca agtgcggagg ccttggcccg gcacgcgcaa attttatgag
      421 tccgcgcagc tgtccgcagt gcggcacgca aatacgctga ttttctagcg cggatctttc
      481 gccaactcct tccttccaag cccagccaca atggaccgat ctcctcttac acccacgctc
      541 agcggacaca ccgccttcat gaaattgctc gcgcaggaag gcgtgactca tttgttcggc
      601 aatccaggca ccacagaact cgccatcatg gaagccatgt cgcagcaatc cgatattgct
      661 tacgtactgg gcttgcagga atcggtggtg gtcgggatgg ccgatggcta tgcccgggcc
      721 tcgaaccagc tcgctgcctg taacgtgcat gttgcccccg ggcttggcaa tgccatgggt
      781 gcgctctaca acgcgaaatt ctatggttcc ccggtactga tcactgcggg tcagcaggaa
      841 cagggccacg gattgatgga accgatgctc tacgacccgc tggtgccaat cgcgcaaccc
      901 atggtgaagt gggctgtcga cgttgctcgc gtgcaggact tgccgcgcat cgtgcatcgc
      961 gccgtgaaac tggcgttgac tcctccgacc ggcccggtgt tcatcagcct gccgggcgat
     1021 attctggatg cgcaagcgcc gctggacctt ggctatccca cgcgcgtgga tgcggaagtt
     1081 cgtccctcgg acgcggcgct cgcccgcctg gcagatcgac tgctcgcggc ccgcaatcca
     1141 gtattgattg ccggccatga gctcgccacg cgcaatgccc tgcaagaagc cgcacaactg
     1201 gccgaaatcc tgggcgcggc cgtgtggcag cagacggtgc cgtatgccgc gcatttcttg
     1261 tccgaacatc ccgcgttcgt gggccagctg acccgcagcc agacgcaggt gcgcgcctgg
     1321 ctgcaaccgc atgatctcgt ggtgtgcttg ggcgccgatg tcttgcgcat gtcggtgcat
     1381 aacccaatcg atgcgctgcc agatggcatg ccgatcgtgc agatttccga acgcgattgg
     1441 gaactcggca aaaattatcc gacggacatc gcgatcaacg ccaatgtgaa acagagtttg
     1501 ctagcgctaa attcggtcat gcgcgcgcgc atgactgccg ctcagaaatc ggcggccgca
     1561 gcgcggctcg ccgaaattgc caagaacaac tggagcgtga agcgcgcacg agcatgcgag
     1621 gatgcggcga aactcgaatc cacacggccg atggatccta aagtcctgat gatgcgcatt
     1681 gctgaagcgc tgccgcgcaa cgtcgtcgtc gtcgaagaag gactcacctc gacgtttccg
     1741 ctgcttaatt ttctgccgct gcgcgatcat cagggtttct atgggctcgc atccggcggc
     1801 atcggctttg ccatggcggg tgctgttgga atcagcatcg ctttgccgga ccgccctgtc
     1861 gttgcgatcg tcggtgatgg cagcgcgatg tacaccatcc aggcactgtg gaccgcagcc
     1921 cacctgaagc tgccgatcac ctatgtcatt ccgaacaatc gcagttaccg catcctgaag
     1981 gagcggcttg tatccttccg caagaccgac aagttcgtcg gcatggacat ggacgatccg
     2041 gccatcgatt tcgtctcact cgcacaatcc atgggcgtga aggcgcagcg catcacggat
     2101 caacaggatg tcggcccggc cttgcgagag tgcatttcca gcggtggtcc aaggttgctg
     2161 gatgtaagcg tggcgaatgg gtttggacga tgacgacgtg gatccgtgct gagtactcag
     2221 tgccggagat tgcatgtttg tggagtcaca attgatcata caatggcttc aaactcccca
     2281 gcgcagcgct gcagtatgca ctggtgcatc ccagaggcgt ttgagttcgc tgtcgagcat
     2341 ggtcacagtc accgagccgc ccgccatatc gagcgaggtc acgtagttcc caaccaacga
     2401 cctcactacg cggacgctag ctttggtcag aaatccatgc gccgcgtgat acaagatata
     2461 cagctccatc agcggtgtgc cgccgaagcc gttcacaagc aaga
//
LOCUS       DATMZQ010000373        16243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_644168, whole genome shotgun sequence.
ACCESSION   DATMZQ010000373 DATMZQ010000000
VERSION     DATMZQ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644168"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VN496_12025"
     CDS             <1..563
                     /locus_tag="VN496_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXF17729.1"
                     /translation="IEVDTPMVWRGPMVTQALEQLLRDTQWKDLDYLVVDLPPGTGDI
                     QLTLSQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVGVPILGLVENMSVHICSNCGY
                     EEHIFGEGGAARMSMDYKVNVLGSLPLDIKIREQADSGKPTVVAEPDSRSAQIYKEIA
                     RKVAARIAELAKDHSGAFPKIVIQNT"
     gene            677..1249
                     /gene="dcd"
                     /locus_tag="VN496_12030"
     CDS             677..1249
                     /gene="dcd"
                     /locus_tag="VN496_12030"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HXF17730.1"
                     /translation="MSIKSDNWIRRMAAEHRMIEPFEPGQVREINGRRIVSYGTSSYG
                     YDIRCSRDFKVFTNINSTTVDPKNFDEKSFVDVSGDVCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPFEPEWEGFVTLEFSNTTPLPAKIYANEGVAQVIFF
                     ESDPDDVCETSYRDRGGKYQGQQGVTLPKI"
     gene            1335..3590
                     /locus_tag="VN496_12035"
     CDS             1335..3590
                     /locus_tag="VN496_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HXF17731.1"
                     /translation="MKFRFPVVIIDEDYRSENTSGLGVRALAKALEDEGMEVLGVTSY
                     GDLSSFAQQQSRASAFILSIDDEEFGAGSEEETNTALKTLRAFVKELRFRNADIPIYL
                     YGETRTSRHIPNDILRELHGFIHMFEDTPEFVARHIIREARAYLDGLSPPFFRALVHY
                     AQDGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHSGPV
                     AASERNAARIFNADHCFFVTNGTSTSNKVVWHANVAPDDIVVVDRNCHVSILHAITMT
                     GAIPVFLTPTRNHLGIIGPIALDEFRPENIQKKIEANPFARAVKRKKPRILTITQSTY
                     DGVVYNVEMLKDTLNSSIDTLHFDEAWLPHATFHEFYRDMHAIGNNRPRTKDAIIYAT
                     HSTHKLLAGISQASQILVQESQTRKLDRHAFNEAFLMHTSTSPQYAIIASCDVAAAMM
                     EPPGGTALVEESIIEALDFRRAMRKVDEEFGKDWWFKVWGPEKLAREGMGSRDDWILK
                     SNEKWHGFGNLAPGFNMLDPIKATVITPGLDVSGKFAKTGIPAAIVTRYLAEHGVIVE
                     KTGLYSFFIMFTIGITKGRWNTLVTALQQFKDDYDKNQPLWRILPEFCQAHPRYERLG
                     MRDLCEQIHGMYKANDVARLTTEMYLSDMQPSMKPSDAFAAMAHREIDRVEIDRLQGR
                     VTSVLLTPYPPGIPLLVPGERFNDTIVHYLQFAREFNRSFPGFETDIHGLVEENSGGE
                     LRYFVDCVRQT"
     gene            complement(3669..4160)
                     /locus_tag="VN496_12040"
     CDS             complement(3669..4160)
                     /locus_tag="VN496_12040"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HXF17732.1"
                     /translation="MPTPRISIIVAMARNGVIGANNALPWHLSADLKRFRALTMGHHI
                     VMGRRTFESIGRVLPGRISVVVTRNPEFRHAGVLTAPSLSEAIERSSDDTEIFIIGGA
                     AIFRDALPVATRIHATELALDYEGDTFFPAFDRTQWRETSAEPFSEGELRGRFVTYDR
                     KST"
     gene            complement(4191..4985)
                     /locus_tag="VN496_12045"
     CDS             complement(4191..4985)
                     /locus_tag="VN496_12045"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXF17733.1"
                     /translation="MRQYLDLMRHILEHGNRKQDRTGTGTLSVFGPQLRFDLQAGFPL
                     LTTKKVHIKSIIHELLWFLKGETNTRYLREHGVTIWDEWADANGELGPVYGYQWRSWP
                     APDGRHIDQISKVIEEIKRNPDSRRLLVSAWNVADLDKMALMPCHAFFQFYVANGKLS
                     CQLYQRSADLFLGVPFNIASYALLTLMVAQVCGLRPGEFVHALGDTHLYSNHIEQARE
                     QLGREPRRLPVMKLNPQVTNIFDFKYEDFTLEAYDPYPAIKAPVAV"
     gene            5102..6259
                     /gene="aroC"
                     /locus_tag="VN496_12050"
     CDS             5102..6259
                     /gene="aroC"
                     /locus_tag="VN496_12050"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXF17734.1"
                     /translation="MSGNTIGKLFCVTSFGESHGPAIGCVVDGCPPGLLLTPADIQKD
                     LDRRKPGTSRHVTQRHESDTVDILSGVFEGRTTGTPIALLIRNEDQRSKDYGNIAETF
                     RPGHADYTYWQKYGIRDYRGGGRQSARETAVRVAAAAIARKWLAEHFGVTIRGYMSQL
                     GTIRIAFKHWEAVNGNIFFSPDPDIVPQLEQFMDRLRKSGESCGAKITAVAQNVPVGW
                     GEPVYDKLDADIAGAMMGINAVKGVEIGSGFASVEQKGTEHSDEMTPQGFLSNNAGGI
                     LGGISTGQDIVVNIAVKPTSSIRLPRRSIDKAGAPTIVETTGRHDPCVGIRATPIAEA
                     MLALVLMDHALRHRAQNADVRTSTPRIASQAPATAGRAQAVAANNPDPDEA"
     gene            6272..6814
                     /locus_tag="VN496_12055"
     CDS             6272..6814
                     /locus_tag="VN496_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17735.1"
                     /translation="MSWLERVGEINAQEWGPVGTAALVASSLLVGLVCYLANAGERWV
                     YILDTANLAFHEAGHPLFGLLFGSRMTVYGGTLGQLVFPTVAIVMFWQRRETVSFALC
                     AAWLFENFWNIARYMADARAHMLPLVGGGEHDWTEIFSRWHMLQRDTSVATVVSVLAW
                     IGIGVTWGWLAWHWWRDRNE"
     gene            6822..7982
                     /locus_tag="VN496_12060"
     CDS             6822..7982
                     /locus_tag="VN496_12060"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17736.1"
                     /translation="MLRGSFYQRLSAFYFSYFAFIGAFAPYFALYLKSLGYSASEIGL
                     LLAVNPVARIFGPNFWGWLSDHYRARSKLIRGTAVGTAVVFIALFFNHGFAWMFIALM
                     LLNVFWCGVLPLAESATLNLVGSKVGAYGRVRLWGSVGFVAVVIAAGYLLDVFGVRAL
                     APLVLSVLTLMAISTFFLPRDREPSHHAEHTSILIILRRPEVIALMTGFFLMQIAIAP
                     YNAFYSIHLVEAGYTKKAVGWLWSLGVLAEIGLFMLLPRLMRAYSLNQILMFSFGCAF
                     VRLLMIGWGVGSLAVLMAAQLLHAITFGAFHSAGVAMMHQIFRGRNQARGQAIYTSLG
                     YGLGGSLGTLMAGYSWDTLGAPWTFTFAAIAALLALIVVASRPVSLPVNAKV"
     gene            8044..9453
                     /gene="leuC"
                     /locus_tag="VN496_12065"
     CDS             8044..9453
                     /gene="leuC"
                     /locus_tag="VN496_12065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXF17737.1"
                     /translation="MNPQTLYDKLWASHLVRQEPDGTALIYIDRHLVHEVTSPQAFEG
                     LKLAGRKPWRIDSVVATADHNTPTQHWDQGIKDPISRLQVETLDANMREFGAKVYFPF
                     LDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQCL
                     VQKKMKTMLIEVDGALPRGVTPKDVALAVIGSIGTAGGSGYAIEFGGSTIRAMSMEGR
                     MTLCNMAIEAGARAGMIAVDQTTIEYLRGRTFAPKGELWDKATAYWKTLRSDENARFD
                     RVVQLDAREIKPQVTWGTSPEMVVPVDGRVPDPDREKDETKREGMERALTYMGLKPNT
                     QITDIRIDKVFIGSCTNSRIEDLRAAAHIVRGRRVASNVRLAMVVPGSGLVKAQAEKE
                     GLDKIFLAAGFEWRQPGCSMCLAMNDDRLEPGERCASTSNRNFEGRQGSGGRTHLVSP
                     AMAAGAAIAGHFVDVRRGG"
     gene            9453..10094
                     /gene="leuD"
                     /locus_tag="VN496_12070"
     CDS             9453..10094
                     /gene="leuD"
                     /locus_tag="VN496_12070"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXF17738.1"
                     /translation="MRKFTRLEGLVAPLDRANVDTDAIIPKQFLKSIKRSGFGVNLFD
                     AWRYLDRGEPGMDNGSRKRNPDFVLNQSRYQGASILLARKNFGCGSSREHAPWALDDY
                     GFRALIAPSYADIFYNNCFKNGLLPIVLDEKHVDRLFDDVAAFPGFRLVLDLGAQTVS
                     YADGSQVFGFEVDGFRKHCLLNGLDDIGLTLTHIDEIKTFEAKRRLEQPWLFS"
     gene            10164..11234
                     /gene="leuB"
                     /locus_tag="VN496_12075"
     CDS             10164..11234
                     /gene="leuB"
                     /locus_tag="VN496_12075"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXF17739.1"
                     /translation="MKIAVLPGDGIGPEIVKQALKVLRHLARDGLKIEIEQAPIGGAG
                     YDAAQDPLPQKTLALAKASDAVLLGAVGGPKYDALPRAMRPEQGLLRIRKELGLFANL
                     RPALLYEELARASSLKPEIVAGLDIMILRELTGDIYFGQPRGTRQTESGEREGFDTMR
                     YSESEIRRIATIGFGIARQRGRKLCSVDKANVLDTSILWREVVAEVGKSFPDVALSHM
                     YVDNAAMQLVRAPKQFDVILTGNMFGDILSDEASMLTGSIGMLPSASLNADNKGLYEP
                     IHGSAPDIAGRDIANPLATILSLAMMMRYSFRNEEAAARIESAVKRVLKQGLRTADIY
                     EAGTTKVGTEEMGNAVSTALQS"
     gene            11273..12391
                     /gene="asd"
                     /locus_tag="VN496_12080"
     CDS             11273..12391
                     /gene="asd"
                     /locus_tag="VN496_12080"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXF17740.1"
                     /translation="MTTVGLVGWRGMVGSVLMQRMQQERDFDLVDPVFFTTSQVGTEG
                     PRIGKAVTVLKDARSIKALKPMDVIVSCQGGDYTNEIYPRLRAEGWSGYWVDAASALR
                     MQKDAVIILDPVNRDVIDAALDSGIKNFIGGNCTVSLMLMGMHGLFKAGVVEWITAMT
                     YQAASGAGAQNMRELLSQMGQLHATAHSLLDDPQSSILDIDRKVADAQCARNFPTEHF
                     GVPLAGSLIPWIDKDLGNGQSKEEWKAQAEGNKILGREADPIPMDGICVRIGAMRCHS
                     QALTIKLRRDLPLEQIEGLLGTANRWTKVIPNNKEDSNRELTPAAVTGTLNVPVGRLR
                     KLPMGGEYLAAFTVGDQLLWGAAEPLRRLLRILLERRA"
     gene            12621..15260
                     /locus_tag="VN496_12085"
     CDS             12621..15260
                     /locus_tag="VN496_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HXF17741.1"
                     /translation="MSALGEPLKAEVELVAEKNEIGSLAVRLANREAFERAGLTYTSL
                     VAGIKATVEKRGSGEPYVQLVSTQPITEPFVDLLIELTWSSGRISREFTALLDPPAVI
                     AERQKQNAAAPEVRAAPKTQQADAVAVPPPSETTKPEVPKPEVSQPEAEKTEAPQATS
                     SAQPVPETAPAPPPASAPPAPVETIGGSQPTLFGEGSALPSGAHSAAMADAYGPVRSG
                     DTLGKIALATKPPEFTLEQMLVLLTRNNPRAFSGKNMNRLKTGKILHLPTSDELASLS
                     PAEASKEVKIQARDWRAYREQLAASAAQSMPADQQAEQSAAGKVGASTQEKAASPAEQ
                     PKEVLKLSKNEPSAGVPGSDAKASARIRALEEEVVAREKAVKESDSRVAKLEKQITDL
                     KALLEMKSKSMADQQKPPGTPAAGAQAPDKSAIQAPAAPTPEATPAATAPVTPTPPSP
                     GPKNESAPAPPQPQPLPPKPAAPKTLPAPPHEASLMDQVMDQPAYLGAAIGVLIIIGA
                     IAVRAVKRRRESKSNAEESGPARAVATSSPEAYAESTYSGSQDSTAEVTKKHSEEVDP
                     VAEAEIFLAYGRDTQAEELLKEALENAPARHEIRLKLLQIYANRKDAKSFEKVARDLQ
                     QATGGTGAIWEQAVALGYQLDPDNARYASGKSAVSNATPIAGPSTAENVDFNIGSDDA
                     AMVTPTDIDLGDTDNAFARTQIIDPADPGQDTATSLEPVATTMDFNVDLPPVEQAAME
                     NVQAAQTAHSSGIDFDIDLNALTSGPDTGKPATSQSIEPGIDFDMSGPALETPTEPRS
                     AGPDIDLSGISLDLGTDTAPTGSPGGKDDHWYDVQTKFDLAKAYQEMGDKDGAREILK
                     EVLAEGDAEQKTAANSVLSTLDS"
     gene            15362..16171
                     /gene="truA"
                     /locus_tag="VN496_12090"
                     /pseudo
     CDS             15362..16171
                     /gene="truA"
                     /locus_tag="VN496_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522025.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
BASE COUNT         3503 a         4539 c         4841 g         3360 t
ORIGIN      
        1 tgatcgaggt cgatacgccg atggtctggc gcggaccgat ggttacgcaa gcgctcgaac
       61 agctgctgcg ggatacgcag tggaaggatc tggattatct ggtggtcgac ctgccgcccg
      121 gcaccggcga tattcaacta accctttcgc agaaggttcc agtgacaggc gcggttatcg
      181 tcaccacgcc gcaggacatt gcccttatcg acgcacgaaa agggctgaag atgttcgaga
      241 aggtcggcgt gccgatcctg gggctggtgg agaatatgag cgttcatata tgctcgaatt
      301 gcggctacga agaacatatc tttggagaag gcggcgcggc gcgcatgagc atggactaca
      361 aagtcaatgt gctgggcagc cttccgttgg acatcaagat tcgtgaacag gcagattccg
      421 gcaagccaac agtggttgcc gaaccggaca gccgctcggc acagatctat aaggagattg
      481 cccgcaaggt cgctgcgcgc attgccgaac tcgcgaagga tcatagcggc gcgtttccaa
      541 agatcgtcat tcagaatacg tgaggcgatg gacatgactg caaagggcgc ggagtgaacg
      601 agcatcggat tcgatcctgg cccttccgcg ttgcctgtcc tccttcgttg ctccaccctt
      661 actcctatgc ccagccatga gtatcaaatc cgacaattgg atccgccgga tggcagccga
      721 gcaccgcatg atcgaaccat tcgaaccggg gcaggtcagg gagatcaatg gccggaggat
      781 cgtgtcttac ggaacctcca gttacggtta tgacatccgc tgttcgaggg atttcaaggt
      841 ttttacgaac atcaattcga ccacggtcga cccgaagaac ttcgacgaga agtccttcgt
      901 cgatgtcagc ggggacgtct gtattattcc gccgaattcc ttcgcgctgg cgcggacagt
      961 ggagtatttc cggatcccgc gtaatgtcct gacgatttgc cttggcaagt caacctatgc
     1021 gcgttgcggc atcatcgtaa atgtgacgcc gttcgagccc gaatgggaag gcttcgtgac
     1081 gctggagttc tcgaacacta caccgctgcc ggccaagatc tacgccaacg aaggcgtggc
     1141 gcaagtcatc ttttttgaat ccgacccgga tgacgtctgt gagacttctt accgcgaccg
     1201 cggtggcaag taccagggtc aacagggcgt tacgctcccc aagatctgaa caactacccg
     1261 ccagtggcga cgccacatct cgcaatgtcc gcccaccggc cttcagcacc ggtgtttccc
     1321 gcagggagat ccgcatgaaa tttcgctttc cggtcgtcat catcgatgaa gattaccgct
     1381 cggagaacac cagtggcctg ggagtccgcg cgcttgccaa ggcgctcgaa gacgaaggca
     1441 tggaagtgtt gggcgtcacc agttacggcg acctgtcgtc tttcgcgcaa cagcaaagcc
     1501 gcgcctccgc attcatactt tcaatcgacg acgaagaatt cggtgcgggc tcggaggagg
     1561 agaccaacac ggccctgaaa acgctgcgtg cgttcgtgaa ggagttgcgt ttcaggaacg
     1621 ccgacattcc gatttacctg tacggcgaga cgcgcacttc gcgacacatc ccgaacgaca
     1681 tcctgcgcga gctgcacggc ttcatccaca tgttcgaaga cacgccggaa ttcgtcgcac
     1741 gccacatcat ccgcgaagcg cgggcttacc tggatggatt gtcgccgccg ttttttcgcg
     1801 ccctggtgca ctacgcccag gacggttcct attcatggca ctgccccgga cactcgggcg
     1861 gagtcgcctt cctgaagagt ccggtcggtc agatgttcca ccagtttttc ggagaaaaca
     1921 tgctgcgcgc cgacgtttgt aatgcagtgg atgaactcgg ccagctgctg gaccatagcg
     1981 gaccggtggc tgcctcggag cgcaatgccg cgcgcatttt caatgcggat cactgtttct
     2041 tcgtgaccaa cggcacatcg acgtccaata aagtggtttg gcatgcaaac gtggcgccgg
     2101 acgatatcgt ggtagtggac cgcaactgcc atgtgtccat tctgcatgct attacgatga
     2161 ccggcgccat cccggttttt ctcacgccga ctagaaacca cctcggcatc atcggaccga
     2221 ttgcgctgga tgaattcagg cctgaaaaca tccagaagaa gatcgaggcg aatccgtttg
     2281 cgcgcgcggt aaaacgcaag aagccacgca tcctgacgat tacgcagagc acctatgacg
     2341 gcgtggttta caacgtcgaa atgctcaagg acacgctgaa ttcgtcgatc gacacgctac
     2401 atttcgacga agcctggctg ccgcacgcga cgttccacga gttttaccgg gatatgcatg
     2461 caatcggcaa taaccggccg cgcaccaagg acgccatcat ttacgcgacc cactccacac
     2521 acaaacttct ggccgggatc tcccaggcgt cgcagatcct ggtgcaggaa tcgcagaccc
     2581 gcaagctgga ccgccatgca ttcaacgaag catttttaat gcatacgtca acttcgccac
     2641 agtacgcgat catcgcctcc tgcgatgtgg cggcggccat gatggaacca ccgggcggca
     2701 ctgcgctggt ggaggaatca attatagagg cgcttgattt ccggcgcgcc atgcgcaagg
     2761 tagatgaaga attcggcaag gactggtggt tcaaggtttg ggggccggag aaactggctc
     2821 gggagggcat gggatcgcgc gatgactgga tcctcaaatc gaacgaaaag tggcacggct
     2881 tcggcaatct ggcgcctggg ttcaacatgc tcgacccgat caaggcgacg gtcatcactc
     2941 cggggctgga cgtgtccggc aaattcgcca aaaccggcat cccggcggca atcgtgacgc
     3001 gctacctggc cgagcatggc gtcatcgtgg aaaaaaccgg cctgtattct tttttcatca
     3061 tgttcaccat cggtatcacg aagggccgct ggaacacact ggtgaccgcg ctgcagcagt
     3121 tcaaggacga ttacgacaag aaccagccgc tgtggcgcat cttgccggag ttttgccagg
     3181 ctcatccgcg ctatgagagg ttgggcatgc gtgatttatg cgagcagatc catggcatgt
     3241 acaaggcgaa cgacgtggcg cgcctcacca cagaaatgta tctgtccgac atgcaaccct
     3301 cgatgaaacc ttccgacgcc tttgcagcca tggcgcaccg ggaaatcgat cgcgtcgaga
     3361 tcgaccggct gcagggtcgc gtaaccagtg tgctgctcac gccttatcct ccgggaatcc
     3421 cactgcttgt ccccggagag cgtttcaacg atacgatcgt gcattacctt cagttcgcgc
     3481 gcgaattcaa ccgctcgttc cccggtttcg agacagatat ccatggtctg gtcgaggaga
     3541 attccggcgg cgaactgcgc tatttcgtcg attgcgtgcg ccagacctga ggtgtttggc
     3601 gcaactccct gcgacatctg tcgtggttag tgagagtacg ccgcccgcgt actgcgcgga
     3661 ctaactggtc aagttgattt tctgtcgtag gtcacaaaac ggccacgcag ctccccttcc
     3721 gaaaaaggct ccgcgctcgt ctcccgccac tgggtgcgat caaaagccgg aaagaacgta
     3781 tctccctcgt agtcgagcgc gagttccgtg gcgtgtatgc gggtcgccac aggcagggcg
     3841 tcacggaaga ttgctgctcc gccgatgata aagatttcag tatcgtcact gctgcgctcg
     3901 attgcttcac tcaggcttgg agccgtcaaa acacctgcat gtcggaactc tggattgcgc
     3961 gtaacgacca cactgatcct gcccggtaat acgcggccga tggattcaaa cgttctgcgg
     4021 cccatgacga tgtggtgtcc catggtaagg gccctgaagc gtttgagatc ggcagaaagg
     4081 tgccacggca gcgcattgtt ggcgccaatg acgccgttgc gcgccatcgc aacaatgatc
     4141 gaaatgcggg gtgtgggcat ggcctggttt gatcgccgac cagcgaccgc tcacacagca
     4201 accggcgcct tgatcgcagg gtacgggtcg taggcttcga gcgtgaagtc ttcgtatttg
     4261 aaatcgaaga tattggtgac ctgcggattg agtttcatca ccggcagtct gcgcggttcg
     4321 cgcccaagtt gctcgcgagc ttgttcgatg tggttggaat agagatgtgt atccccaagc
     4381 gcatgcacga attcgccggg ccgcagacca cacacctgcg ccaccatcaa ggtgagtaag
     4441 gcataggacg cgatgttgaa gggcacgccg agaaacagat cggcgctgcg ctggtagagt
     4501 tgacaggaca gtttgccgtt ggccacgtaa aactggaaaa aggcatggca cggcatcagc
     4561 gccattttgt ctagatcagc gacgttccag gcggaaacca gcaaacgtct cgagtctgga
     4621 ttgcgcttga tctcctcgat caccttggaa atctgatcga tatgtcgtcc atccggtgcc
     4681 ggccaggagc gccattggta gccataaacg gggcccagtt cgccattggc atcagcccat
     4741 tcatcccaga tcgtgacgcc gtgctcacgc agatagcgcg tgttggtttc gcctttcagg
     4801 aaccacagca attcgtgaat gatcgacttg atgtgcactt tcttggtggt gagcagcgga
     4861 aagcctgcct gcaaatcgaa acgcagctgt ggcccgaaca cactcagcgt tccggttccc
     4921 gtacggtcct gcttgcggtt tccgtgctcg aggatatggc gcatgaggtc gaggtactgt
     4981 cgcattgcgc tggccgtgat cgagttggca aaagcggaag tttagtgcac ctgccgggcg
     5041 gatggaatga agcagcgaac ccctatgata aactcaaaaa acttttttct ggctcaacgt
     5101 catgtcgggc aacaccatcg gcaagctgtt ctgtgtcact tcgtttgggg aaagccacgg
     5161 gccggccatc ggctgcgtcg tggatggctg tccgcccggg ttgctgctta caccagccga
     5221 tatccagaaa gacctcgatc gccgcaagcc ggggacatcg cggcatgtga cccagcggca
     5281 tgaatccgat acggtggaca tactgtccgg cgtgttcgaa ggtcgcacga ccggaacgcc
     5341 gatcgccctg ctgatccgca acgaagatca acgcagcaaa gactacggca atatcgcgga
     5401 aacttttcgt cccgggcatg ccgattacac ctactggcaa aaatacggca tacgtgatta
     5461 tcggggaggg ggacggcagt cggcgcgtga aaccgccgtc agggttgccg ctgccgcgat
     5521 tgcacgaaaa tggctcgcag aacatttcgg tgtaacgatc cgcggctaca tgtcccagct
     5581 tggaacgatt cgcattgcgt tcaagcattg ggaagcggtg aacggcaaca tttttttttc
     5641 gccggatccg gatattgtcc cgcagttgga gcaattcatg gaccgtctgc gcaagtcggg
     5701 cgaatcgtgc ggcgcaaaaa taaccgcggt tgcgcaaaac gttccggttg gctggggaga
     5761 gccggtgtac gacaagctgg atgcggatat tgccggcgcg atgatgggaa tcaatgcggt
     5821 gaagggcgtg gaaatcggct ccggatttgc gtcagtggaa cagaagggca ctgagcacag
     5881 cgacgaaatg acgccccaag gatttttgtc caacaacgcg ggcggcatac tgggcggcat
     5941 ttccactggg caggacattg tcgtcaatat cgccgtaaaa cccacgtcca gtatcagact
     6001 gccgcgccgt tccatcgata aagccggtgc tccgacgatc gtcgaaacca ccggccgcca
     6061 tgatccttgc gtgggcatcc gcgctacgcc gatcgcagaa gccatgctgg cgttggtgct
     6121 gatggatcat gctctgcgcc accgggcgca aaacgctgat gttcgtacct ccacgccgcg
     6181 aattgcttcg caggcacccg cgaccgccgg gcgtgcgcaa gcggtggcag ccaacaaccc
     6241 cgaccccgac gaagcctgat cgaaatcatc tgtgtcctgg ctggagcggg taggtgaaat
     6301 caatgcacag gagtggggtc cggtcgggac cgccgctttg gtcgcgtctt cccttctggt
     6361 aggtctggtc tgctatctgg ccaatgctgg cgagcgttgg gtatacatac tggacaccgc
     6421 gaacctggct tttcatgagg ccggacaccc gttgttcggt ctgttgtttg gcagccgcat
     6481 gactgtctac ggcggcacac tcgggcagtt ggtgtttccc acagttgcca tagtgatgtt
     6541 ctggcagcgc cgtgagacgg tttccttcgc attatgcgcg gcctggttgt tcgaaaattt
     6601 ttggaacatc gcacggtaca tggctgacgc acgcgcgcac atgctgccgt tagtcggcgg
     6661 cggcgaacac gactggacgg aaattttttc tcgctggcac atgctgcaac gggataccag
     6721 tgtcgcgact gtggtttccg tcctggcctg gatcgggata ggtgtaacct ggggctggtt
     6781 ggcatggcac tggtggcggg accgaaacga gtaaaggcaa tatgctgcgc ggttccttct
     6841 atcagcggct ctccgctttt tatttttcct atttcgcctt tatcggcgca ttcgcgccgt
     6901 atttcgcgct ttacctgaag tccctgggct attcggcatc cgaaatcggc ttgctgcttg
     6961 cagtcaaccc ggtcgcacgt atcttcggac cgaatttctg gggatggctg tccgaccatt
     7021 accgtgcgcg cagcaagttg atacggggta ccgcagtggg cacggccgtg gttttcatcg
     7081 cgctgttttt caatcacggg tttgcgtgga tgttcattgc cctgatgctg ctaaatgtct
     7141 tctggtgtgg agtgctcccg ctggcggaat ccgcgacctt gaacctcgtg ggatcaaaag
     7201 tgggggcgta tggcagggta cggctgtggg gatcagtcgg gttcgtcgcc gtggtcattg
     7261 ctgccggcta tttgcttgat gtgtttgggg tccgcgctct tgcgccgctg gtgctgtcag
     7321 tgctcaccct catggcgatc agcacttttt ttcttccacg tgaccgtgaa ccttcgcacc
     7381 atgcggaaca cacctcgatt ctgattattc taaggcgacc cgaagtgatt gcgctgatga
     7441 cgggcttctt cctgatgcag atagcgatcg ctccctataa cgcgttctat tccattcacc
     7501 tggttgaggc gggctatacg aagaaagcgg tcggatggtt gtggtcgcta ggcgtcctcg
     7561 cggaaatcgg gctgttcatg ctgctgccgc gtttgatgcg tgcctatagc cttaaccaga
     7621 tcttgatgtt cagttttggc tgcgcctttg tgcgcctgct gatgattggt tggggcgtcg
     7681 ggtcgctagc ggtgctcatg gcagcgcagt tgctgcacgc catcacgttc ggcgcatttc
     7741 actccgcagg tgtcgcgatg atgcatcaga ttttccgcgg tcgtaatcag gctcgcggcc
     7801 aggctattta taccagcctg ggatacggac tcggtggttc gctcggtacg ctgatggccg
     7861 gctattcatg ggacacgctg ggtgcgccat ggacctttac cttcgcggcc attgccgcac
     7921 tgctagcctt aattgtggtg gcgtcaaggc cagtttcctt gccagtcaac gcgaaggtct
     7981 agcgcgcttc gcgccgtcgg ggcgttgcct tgtgtcataa tttctgcttg catcaaaaca
     8041 cttatgaatc cgcaaactct ctacgacaag ctctgggcaa gccatttggt tcggcaggaa
     8101 ccggatggca ccgcgcttat ctatatcgac cgccatctgg tgcacgaagt caccagcccg
     8161 caggcctttg aagggctgaa gctcgccgga cgcaagccct ggcgcatcga ttcggtggtt
     8221 gccactgcag atcacaacac gccaacgcag cactgggatc agggaatcaa agacccgatt
     8281 tcacgcctgc aggttgaaac gctcgatgcc aacatgcggg agttcggggc gaaggtttat
     8341 ttcccgttcc tggacaagcg tcaaggcatc gtgcatgtaa ttggaccgga gcagggcgca
     8401 accctgccgg gcatgaccgt tgtttgcggg gattcgcaca ccagcacgca cggcgccttt
     8461 ggctgcctgg cgcacggcat cggcacgtcg gaagtggaac atgttcttgc cacacagtgc
     8521 ctggtgcaga agaaaatgaa gaccatgctg atcgaagtcg atggcgcgct tccccgcggc
     8581 gtaacgccga aggatgtggc gcttgccgta attggcagca ttggcacggc cggcggaagt
     8641 ggctatgcga tcgaatttgg cggcagcacc attcgtgcaa tgtcgatgga aggccgtatg
     8701 actttgtgca acatggcgat tgaggccggt gcgcgcgctg gaatgattgc agtagaccag
     8761 acaaccatcg aatacctgcg cggccgcacg ttcgcgccga aaggggaact ctgggacaaa
     8821 gcaacagcgt attggaaaac gctgcgcagc gacgagaatg cacggttcga cagggtggtg
     8881 caattggatg cgcgggagat caaacctcag gttacctggg gcacttctcc ggagatggtt
     8941 gtgccggtgg acggccgtgt gccggatccg gaccgggaga aggacgaaac gaagcgcgaa
     9001 ggcatggagc gggcgctgac ctacatgggc ctcaaaccta atacgcaaat taccgatatc
     9061 cggattgaca aggtcttcat cggttcctgc accaattcgc gcattgaaga tttgcgggct
     9121 gctgctcaca ttgtgcgcgg ccggcgtgtt gcttctaacg tacgcctcgc gatggtggta
     9181 ccgggttctg gcctcgtgaa ggctcaggcc gaaaaggaag gtctcgacaa aatcttcctg
     9241 gctgcgggct tcgagtggcg ccagcccggg tgctccatgt gcctggccat gaacgatgac
     9301 cggctcgaac ccggcgagcg ctgtgcgtca acgtcgaacc gtaacttcga aggcaggcaa
     9361 gggtccggag gccgtacgca tcttgttagc ccggccatgg ctgcaggtgc ggcgattgcc
     9421 ggccatttcg tcgacgtgcg gagaggcggg taatgcgcaa gttcacgcga ttggaaggcc
     9481 tggtcgcgcc gctggatcga gccaatgtcg ataccgacgc gattatcccc aagcagtttt
     9541 taaagtcgat caagcgctcg ggttttggcg ttaatctgtt tgatgcctgg cgctacctgg
     9601 atcgcggtga accgggcatg gataacggca gtcgcaagcg caacccggac ttcgtcctca
     9661 accagtcacg ctaccagggc gccagcattt tgctggcgcg aaagaacttc ggctgtggtt
     9721 cctcccgcga gcatgcgccg tgggcactgg acgactacgg ctttcgcgcg cttattgcac
     9781 cgagttacgc cgatattttc tataacaatt gtttcaagaa tggattgttg ccgatcgtac
     9841 tggacgaaaa gcatgtggac cggctatttg atgatgtcgc tgcctttccc ggattccgac
     9901 tcgtcctcga tctgggcgca caaacggttt catatgccga tggcagccag gtattcggtt
     9961 tcgaagtgga tggtttccgc aaacactgcc tgctgaatgg gctcgacgac attggtctga
    10021 ccctgacgca tatagacgaa ataaaaacgt tcgaggctaa acgccgtttg gaacaaccct
    10081 ggctgttcag ttgacggatt agaattgaaa acagcggccg gggcagaaca tcatgccccg
    10141 gtcgtttttt gagagaacca aaaatgaaaa ttgcggtgtt gcctggtgat ggaatcgggc
    10201 cggaaattgt gaagcaggcg ctcaaggtgc tgaggcacct ggcacgtgat ggcctgaaaa
    10261 tcgaaatcga acaggcgccg ataggcggag ccggctatga cgctgcacag gatccgctgc
    10321 cgcaaaagac gctcgcgttg gcgaaggcat cggacgccgt gttgctcggt gctgtcggag
    10381 ggccgaaata cgatgcgctg cccagggcga tgcgtccgga acagggcctg ctgcgcatcc
    10441 gcaaggaact cggattgttt gccaatttgc gccccgcatt gctatacgaa gaactggccc
    10501 gcgcatcgtc gctgaagccc gaaatcgtag cggggctcga tatcatgatc ctgcgcgaac
    10561 tgactggtga tatttatttt ggccaacctc gcggcacccg ccagacggaa agcggcgagc
    10621 gagagggctt cgataccatg agatatagtg aatcagagat ccgtcgcatt gccactattg
    10681 ggttcggcat cgcgcgccag cgcggccgaa agttgtgctc ggtggacaag gcaaacgtac
    10741 tcgataccag catcctgtgg cgcgaagtag tggccgaagt tggaaaaagt ttccccgacg
    10801 tcgcactctc gcacatgtat gtggacaacg cagccatgca gctggtgcgc gcgcccaaac
    10861 agtttgacgt aattctgacc ggaaacatgt tcggcgatat tctttccgat gaggcctcga
    10921 tgctgaccgg ctccatcggc atgttgccgt ctgcctcgct gaacgccgac aacaaggggc
    10981 tgtacgagcc gattcacggt tcggccccgg acatcgccgg gcgtgacatt gccaatcctc
    11041 tggccacgat cctgtcgttg gcgatgatga tgcgctatag cttccgcaac gaagaggctg
    11101 cagcgcgcat cgagtcggcc gtcaagcgcg tgctcaagca gggcctgcgt acggccgaca
    11161 tctatgaggc cggtacaacc aaggtcggta cggaagaaat gggtaatgcc gtgtccacgg
    11221 cattgcagtc atgagccaaa acggcagcta atgttttctg acggaaattg aaatgacaac
    11281 agttggactg gtgggttggc gtggcatggt gggatcggtg ctgatgcagc gcatgcagca
    11341 ggaacgggat ttcgatcttg tcgatccggt gttcttcacg acctcccaag ttgggactga
    11401 aggtccccgg atcggcaaag ccgttaccgt gttgaaggac gcgcgcagca tcaaggctct
    11461 caagccgatg gatgtgattg tttcctgcca aggtggcgac tataccaatg agatttatcc
    11521 gaggcttcga gcagaaggtt ggagcggcta ctgggtagac gctgcttcgg cgctgcgcat
    11581 gcaaaaggat gcggtaatca tccttgatcc ggttaaccgc gatgtgatcg atgcagcgct
    11641 tgactcgggc atcaagaact tcatcggcgg caattgcaca gtaagcctta tgctgatggg
    11701 gatgcatggt ctgttcaagg ctggtgtcgt ggaatggatt acggcgatga cgtatcaggc
    11761 cgcgtccggt gcaggggcgc aaaatatgcg cgaattgttg tcccaaatgg gtcagctgca
    11821 tgcaacggca cattccttgt tggacgatcc gcaatcctcc attctcgaca tcgatcgtaa
    11881 agtggcggat gcgcagtgcg cgcgtaattt tccgaccgag cattttgggg tgccgctcgc
    11941 gggcagcctg atcccctgga ttgacaagga tctgggcaat ggccagagca aggaggaatg
    12001 gaaagctcag gcggaaggca acaagatcct tgggcgcgaa gcagatccga ttccaatgga
    12061 cggtatctgc gtgcgcatag gcgcaatgcg ctgtcacagc caggcattga ccatcaaatt
    12121 acggcgagat ttgcctctcg agcagatcga aggcctgctg gggactgcca atcgctggac
    12181 aaaagtgatt ccgaacaata aagaagattc caaccgtgaa ctcaccccgg ctgcagttac
    12241 cggaacgtta aatgtcccgg tggggcgcct gcgcaagctc ccaatgggcg gcgaatatct
    12301 cgcggctttt accgtaggag atcagctgct gtggggggct gcggagcccc ttcgccgcct
    12361 gttacggata ctgcttgagc gccgcgctta gccacggcgt ttttgcaatg gagcccaaaa
    12421 gccgtgaaac cttgcgcaat gtgaggcgaa agatgagctt gcatctctaa atccatgatg
    12481 ttattttaat tacttcgtac aaaaaacgat tcagggtgag cgctttgggc attacgtctc
    12541 ttcgctcgtg gttaatcgcc gcgtcgttgc tttcgatctg cgccgtatcc cacgcagcgg
    12601 gtctcggaaa gctacgtgtc atgtcagcgc ttggcgaacc gcttaaagcg gaggtcgaac
    12661 tcgtcgctga gaaaaatgaa atcggttcgc tggccgtgcg actggcgaat cgagaagcgt
    12721 tcgagcgcgc cggtcttacc tatacaagtc tggttgcggg catcaaggca accgtggaaa
    12781 agcgcggtag cggcgaacct tacgtgcagc tggtatcaac tcagccgatc accgagccat
    12841 ttgtcgattt gttgattgaa ttgacatggt cctccggtcg tatcagtcgc gaattcacgg
    12901 cacttctgga tccgccggca gttatagctg aacgccaaaa gcagaatgcg gcagcgcctg
    12961 aagtccgtgc cgcccccaag acacaacaag ccgatgctgt ggcggttccc ccgccgtcgg
    13021 aaaccacgaa acccgaagtg ccgaagcccg aggtgtcgca accggaagcc gagaagacag
    13081 aagcgccgca ggccacgtct tcagcgcagc cggtgccgga gacagctcca gcgccaccgc
    13141 ccgcctccgc accaccagcc ccggttgaaa ccataggcgg ttcccagccg acgttgtttg
    13201 gcgaaggaag tgccttgcca tctggggcgc attctgccgc catggcagat gcctacgggc
    13261 cggtcaggag tggagatact ctcggaaaga ttgctctggc cacgaagccg ccggaattta
    13321 cgctcgaaca aatgctcgtt ctgctcacgc gcaataatcc gcgcgcgttc tccgggaaaa
    13381 acatgaaccg gctgaagacc gggaagattc ttcatttgcc gacctctgac gagctggcaa
    13441 gcttgagccc ggcagaagcc agcaaggaag taaaaataca ggcacgcgat tggcgtgcct
    13501 accgcgagca attggccgcc tcggcagcgc aaagcatgcc ggcggaccag caggcggaac
    13561 aatctgccgc aggcaaggta ggcgcgagca ctcaagaaaa agctgcgtct ccagctgaac
    13621 agcccaagga agttttgaaa ctgtcgaaaa atgaaccttc ggccggtgtg ccgggcagcg
    13681 atgcaaaggc gtcagcccgt attcgagctc tcgaagaaga ggtagtggcg cgcgaaaagg
    13741 ccgttaaaga atccgacagc cgcgtggcca agctggaaaa acaaataacg gatctgaagg
    13801 ctttgctgga aatgaaaagc aaaagcatgg cggatcagca aaaaccacct ggcacgccag
    13861 ctgctggagc ccaggcgcca gacaaaagcg cgattcaagc gcccgctgcg ccgacacccg
    13921 aggcaacgcc tgcagcgacg gcgcctgtca cgccgacgcc accctcccca gggcctaaaa
    13981 acgagagtgc tccggcgcct ccacaacccc agccgttgcc gccgaaacca gctgctccaa
    14041 aaacgctgcc cgctccgccg catgaagcca gtctgatgga ccaggtgatg gatcagcctg
    14101 cttatctcgg cgcggcgatc ggtgtgctga tcattattgg ggccattgcc gtacgcgccg
    14161 tgaagcggcg aagggaatcg aagtccaacg ccgaagaatc tggccctgcg agggctgtcg
    14221 ccacaagctc gccagaggca tacgctgagt cgacgtactc cggcagccag gactccaccg
    14281 ccgaagtcac caaaaaacat tccgaggaag ttgatccggt agctgaagcg gaaatcttcc
    14341 ttgcctacgg tcgcgatacg caagcagaag aactgctcaa ggaggcactg gagaacgctc
    14401 ctgcccggca tgagattcgt ctcaagctgc ttcagattta cgccaatcga aaagatgcca
    14461 aatcgttcga aaaagtagcg cgtgacctgc aacaggctac tgggggaacc ggcgcaattt
    14521 gggaacaggc ggtcgctctt ggctaccaat tggatccgga caacgcgcga tatgcttcgg
    14581 gaaaatccgc agtgtccaac gcgacgccaa tcgccggccc ttccactgcc gaaaacgttg
    14641 atttcaacat cggttccgac gatgcggcga tggtcacccc gactgacata gacctgggcg
    14701 acacggataa tgcattcgcc cggacgcaaa tcatcgatcc tgccgatccc ggtcaggaca
    14761 cggctacatc gctcgaaccg gtcgcgacga cgatggattt caatgtcgac ttaccgccag
    14821 tggagcaagc ggccatggaa aatgtgcagg ccgcccagac tgcgcattca tccgggattg
    14881 atttcgacat cgatctaaat gcgctaacca gcggtccgga taccggtaaa cccgcaacat
    14941 cccaatccat cgaacccggg attgattttg acatgagcgg ccccgcgctc gagacgccca
    15001 ctgagccacg ttcggccgga ccggacatcg atttgtccgg aatcagtctg gatcttggaa
    15061 ccgatacggc gccaacgggt tcgccaggcg gcaaagatga tcattggtac gacgtgcaaa
    15121 ccaagttcga cttggccaag gcataccagg aaatgggcga taaggatggc gcgcgcgaga
    15181 ttctgaagga agttctggca gaaggcgatg cagaacaaaa aactgccgcc aattctgtgc
    15241 tgtctaccct cgactcctga ttgcagcccg aaagggcatt tgccgccgcc accgcttgcg
    15301 gcacaattca atttaatgcc caattgcagt gacccaatca cgggtccctg cggactattc
    15361 tttgagaatc gcgctcggtg tggaatatga cggtaccgcg ttctgcggtt ggcagagcca
    15421 gccgcaggcg tgcggcgtgc aggatgcgct tgaacgcggt ttggctggat tatcgggcga
    15481 gcgcgtaagt acggcttgtg ctgggcgtac tgatgctgga gtccatgcgc tgggccaggt
    15541 tgtccatttc gataccgcgg cagtgcgacc attgaatgcc tgggttcgcg gcaccaacgc
    15601 gctgctgcca gcggccatgt cggtgacctg ggcgaagccg gtaacggaag aatttcatgc
    15661 gcggttttca gcgacagggc gacgctacgt gtattggctg ctgaacaggc cacagcgtcc
    15721 aggtcttttg cataatcgtg tttgatggtt ccatcagacg ttggacatcg aagccatgcg
    15781 acgggcggcc gccttgatcg tcggaagaca cgattacagc gccttccggg cagctgaatg
    15841 ccaggcaaag acaccgtgga aagagcttcg ggaactcaac atatcgcaac ggggcgatat
    15901 gctgcgcttt gaatttgcag cagacgcttt tttgcagcac atggtgcgca acatcgtcgg
    15961 cagcctcgtc tacatcggcg cgggacgcca gccgttccaa tggctgcatg agctattgca
    16021 ggcccgggac cgcacccgcg ccgcgccacc attttcccca gccggcctgt accttgcagc
    16081 ggtacagtac gacagcatgt gggctctacc gccacaaact gacctgcgcg ctgtcgagga
    16141 cacgatgctg gaaggcgcgc actcccaata aggcccgcaa tggcgaccgc cgttaagatc
    16201 tgtggcatta cccgcgtcga ggacgcgctc gtcgcggcga aaa
//
LOCUS       DATMZQ010000374         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3405008, whole genome shotgun sequence.
ACCESSION   DATMZQ010000374 DATMZQ010000000
VERSION     DATMZQ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405008"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..400
                     /locus_tag="VN496_12095"
     CDS             29..400
                     /locus_tag="VN496_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF17742.1"
                     /translation="MAAHTPDWNGENALKPLSSRYVKMEQLAWRKTAFPGVEIKLLLE
                     DKETGLTTNLTRMAPGAVLPLHEHVGVEQTYVLEGRLVDSEGEVKAGEYVWRPAGSRH
                     VASAPEGALLLGMFLQPNRFL"
     gene            495..995
                     /locus_tag="VN496_12100"
     CDS             495..995
                     /locus_tag="VN496_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17743.1"
                     /translation="MIRAGSSIPEEHGIHDIYGRSPVPPQGHDIHGLCGIYAFSPSSI
                     DDNGIPLALDAAPGAPDHHQVQTDRRCALMQIKPIIAAAGVIALMLLGYPAVAAEAAR
                     QSGTAIEQDPEARLGDQHAMKAGRPGTNVAVHNTRPRNDRHKDARKCLNAGNNAQISS
                     CAHKYR"
     gene            complement(1028..>2381)
                     /locus_tag="VN496_12105"
     CDS             complement(1028..>2381)
                     /locus_tag="VN496_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF17744.1"
                     /translation="DLVGMDDIKAEVAQIKDQYQRRAAYAEYGIDKPFNVMFSGPAGT
                     GKTKLASYLAKELRLPILFHSAANLETGFVAGGSNTLNRIMEMAKRRRRCIVFLDEAQ
                     DLFMKRGGRRKFDDDTANTLLSVLDGVRSKQAGEIIWIVASNFNSETMQMDPAMLRRF
                     QMKVDFRMPNREERLAIIDHYLSQRADKVLHDLDLRHLVEVTEGRSPADLETIVNQAG
                     ITAVQVGDMIGEPTLMQAAERMLVGNVNANTTKERERDRRIIAVHEWGHFLVDLEHER
                     KVAGDNWDQILADMKTIKISLKANPRNNALGFVFHKQGINLLKTKADIEHDVRVLLGG
                     MANEELFFGEDGTTNGAHNDITRVTQLLHHAVGEMGMYRKTRLNFGALSPDKSKTGLV
                     DEETRGIMEVQSERLYAETKAMLARHKQLTEHLVSKLLAAGEMSLNDALSEIRRFEAA
                     "
BASE COUNT          465 a          741 c          683 g          492 t
ORIGIN      
        1 gactcatccg caactgaaag tcatcgccat ggctgcccat acccctgact ggaacggcga
       61 gaacgcactc aagccgctct cgtcccgcta cgtgaagatg gagcaactcg cgtggcgcaa
      121 gaccgcattc cccggagtgg aaatcaagct gttgcttgaa gacaaggaga ccggcttgac
      181 gaccaacctt acgcgcatgg cgccgggtgc cgttctgccg ctgcacgagc acgtcggggt
      241 tgaacagacc tacgtgctgg aaggcaggct ggtggacagc gaaggcgagg ttaaggccgg
      301 cgaatatgtc tggcgtcccg ccggcagcag gcacgtcgct tcagcccccg aaggcgcctt
      361 gctgctgggc atgtttctgc agccgaatcg gtttctttga agccgtgaat atcgtgaata
      421 ccgtgaatat cgtcaaggtc gtaaattccg cgaaccgcgg taaacagagg caatcgtaag
      481 gcagtacacc atgcgtgatt cgagccggca gcagtatccc cgaagagcac ggcattcacg
      541 acatttatgg gcgcagcccg gtacccccgc aggggcacga tattcacggc ctttgcggca
      601 tttacgcctt ttccccgtcg tccatcgacg acaacggcat accgttagcc cttgacgcag
      661 cccccggtgc gccggatcat catcaagtgc aaaccgacag gagatgcgcg cttatgcaga
      721 tcaaacccat cattgccgca gcgggcgtga ttgcgcttat gctgcttgga taccccgccg
      781 tggcagccga agcagccagg caatccggca cagcaatcga gcaggatccg gaagcgagac
      841 tgggcgacca gcacgcgatg aaagccggca ggccgggcac caatgtcgcg gtccacaata
      901 cgcggccgcg caacgatcgt cacaaggatg cccgcaaatg cctcaacgcc ggcaacaacg
      961 cccagatctc cagctgcgcg cacaaatacc gctgaccacc gcactttcgc caccgccaga
     1021 aatcacgtca ggccgcctcg aagcggcgaa tctccgacag cgcatcattc aggctcatct
     1081 cgccagcggc gagtagcttg ctgaccagat gttcggtgag ctgcttgtgc cgggccagca
     1141 tcgccttggt ctcggcgtac agccgctcac tctgtacttc catgatgccg cgcgtttctt
     1201 cgtccaccaa tccggtctta ctcttgtccg gagaaagtgc gccgaagttc agacgcgttt
     1261 tgcggtacat gcccatctct ccgactgcgt gatgcaggag ctgcgtcacg cgcgtgatgt
     1321 cgttgtgcgc accgttagtc gtgccgtcct cgccaaaaaa caattcctca tttgccatgc
     1381 cgcccagcag cacgcgcacg tcgtgttcga tgtcggcctt ggtcttcagc agattgattc
     1441 cctgcttgtg gaatacaaag cccaatgcgt tgttgcgcgg gttggccttc agtgagatct
     1501 tgatggtctt catgtccgcg aggatctggt cccagttgtc gcccgccacc ttgcgttcgt
     1561 gttccaggtc caccaggaaa tgtccccact cgtgcacggc aatgatgcga cggtcgcgct
     1621 cgcgctcctt ggtcgtgttg gcgttgacgt tgcccacgag catgcgttcg gcggcctgca
     1681 tcaaggtcgg ctcgccaatc atgtcgccca cctgcacggc ggtgatgccg gcctgattga
     1741 cgatcgtctc gagatcggcc gggctgcggc cttcggtcac ttccaccaga tggcgcaaat
     1801 cgaggtcgtg caataccttg tcggcacgct ggctgagata gtgatcgatg atggccaacc
     1861 gctcttcgcg attcggcatg cgaaaatcca ctttcatctg aaaccggcgc aacatggccg
     1921 ggtccatctg catggtttcg ctgttgaagt tgctggccac gatccagata atttcgccag
     1981 cctgcttgct gcgtacgccg tcaagcacgg ataacagcgt gtttgcagta tcgtcatcga
     2041 acttgcgccg gccgccgcgc ttcatgaaca ggtcctgcgc ttcgtcgagg aatacgatgc
     2101 agcgcctgcg gcgcttggcc atctccatga tgcggttcag cgtgttcgaa ccgcccgcga
     2161 caaatccggt ttccaggttg gccgcggagt ggaacaggat cggcagccgc agttccttgg
     2221 cgagatagct ggcaagtttc gtttttccag ttccggctgg gccgctgaac atcacgttga
     2281 acggcttgtc gatgccgtac tcggcatagg ccgcgcggcg ctgatactga tccttgatct
     2341 gcgcgacttc ggccttgatg tcatccatgc cgaccagatc a
//
LOCUS       DATMZQ010000375         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2713654, whole genome shotgun sequence.
ACCESSION   DATMZQ010000375 DATMZQ010000000
VERSION     DATMZQ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713654"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VN496_12110"
     CDS             <1..1233
                     /locus_tag="VN496_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17745.1"
                     /translation="ITEQLIKSGHKVIGAVRDPASQARTPVGEQTEYIAADFCRDLDP
                     TVWVPRLKGVDIVINAVGIIRETGVQTFAAIHALGPQALFAACQQAGIRRIVQVSALG
                     ADERATSAFHVSKRGADEFLLRLPLSAAVVQPSLVYGEEGASSRLFRRLASMPVILLP
                     GYGEQRIQPIHVHDAALAVTALAISPFTGRMPLVGPQPISLREYLAQLRTAMRLGVPY
                     FVPVPPAVLTGVSAVGAWRWGVPDEAMLSMLERGNTADPAPLAALLGRSPRAVHQFIP
                     AHAAAAVGLAARLSWLAVLLRLSIALVWIVSGVLSFGVYPVHESYALLAQAGIAGAWA
                     VAALYAGAALDLLLGMATLMVSHRRWLWVLQASAIIAYTAIITLRLPAFWLHPFGPVL
                     KNLPMLMAVWIMYELDRR"
     gene            1233..1697
                     /locus_tag="VN496_12115"
     CDS             1233..1697
                     /locus_tag="VN496_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HXF17746.1"
                     /translation="MDYQVVKWLHVLSSTLLFGTGLGSAYYMFFASRTRDPRVIAVVV
                     RHVVVADWLFTATTVVFQPLSGFYLAYLAGFPLHSRWIVWSIVLYLVAGACWLPVVWM
                     QIRMRDMAVRATQQEQALPAQYERYLKRWVLLGIPAFTALVVVFYLMVAKPV"
     gene            complement(1684..2139)
                     /locus_tag="VN496_12120"
     CDS             complement(1684..2139)
                     /locus_tag="VN496_12120"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HXF17747.1"
                     /translation="MSRRSELQSTNRHSEMAGFSGYSRFAKWVSQASGGGAAFCIAVA
                     LVFTWVITGPVFHFSDTWQLMMNTCSSIITLLMVFLIQNTLNRDSTALQIKLDELIRA
                     LEGAQNAMIGLENLDDEQLNRLAQKYADIADRARQESDTRGRHRTARPV"
BASE COUNT          462 a          784 c          793 g          601 t
ORIGIN      
        1 atcacggagc aactgatcaa gtcagggcac aaggtcattg gcgcagttcg cgatccggca
       61 tcacaagcgc gcactcctgt tggagagcaa accgagtata tcgcggcaga tttttgccgc
      121 gacctggatc ccacggtctg ggtgccgcgt cttaaaggtg ttgacattgt catcaacgcc
      181 gtcgggatta ttcgcgagac gggtgtgcag acgtttgcgg cgattcatgc cttgggcccc
      241 caggcgctgt ttgccgcctg ccaacaggcc ggcattcgcc gcatcgtgca ggtgtcagcg
      301 ctgggcgctg atgaacgcgc gaccagcgcg ttccatgtga gtaaacgcgg tgccgacgag
      361 ttcctcctgc gattgccgct ttcggccgcc gtcgttcagc cttcgctcgt gtatggagag
      421 gaaggcgcaa gttcgcgcct gttccggcgt ttggcgagca tgccggtaat cctgctgccg
      481 ggttacggcg aacagcgcat tcagccgatc catgtgcacg acgccgcgct tgccgttact
      541 gcgctggcga tatcgccgtt cacgggccgc atgccgctgg tcgggccgca gccgatttcg
      601 ctgcgcgaat atctcgcgca attacgcacc gccatgcgac tcggcgttcc gtatttcgtc
      661 cccgtcccac ctgcagtcct taccggcgtt tctgcggtgg gtgcctggcg ctggggcgtg
      721 cctgacgaag cgatgctgag catgctggag cgcggtaata cggcggatcc cgcgccgctc
      781 gctgcgctgc tcggccgcag tccgcgcgca gttcaccagt tcattccagc gcacgcagcc
      841 gccgcggtcg ggcttgcggc acgtctttcc tggctcgcag tgttgctgag actcagcatt
      901 gcactcgtat ggatcgtaag cggcgtgctt tccttcggcg tttaccccgt gcatgaaagc
      961 tacgcattgc tcgcgcaagc cggcattgca ggcgcatggg ctgtggccgc gctctacgcc
     1021 ggcgcggcac tggatctgct gctaggcatg gcaacactaa tggtgtcgca tcgtcgctgg
     1081 ctgtgggtgc tgcaggccag tgcgatcatt gcctacacag cgatcattac cctcaggctg
     1141 cccgcgttct ggctgcatcc gtttggtccg gtgctgaaga acctgcccat gctgatggcg
     1201 gtctggatca tgtacgagct ggaccggcgt tgatggacta ccaggtcgtc aagtggcttc
     1261 atgtgctgtc ttcgacgttg ctgttcggca ccggtctggg ttcggcctac tacatgtttt
     1321 ttgcgagccg cacgcgtgat ccccgagtca ttgcggtggt ggtacgccat gttgtcgtcg
     1381 ctgactggct gtttactgca accaccgtcg tgttccagcc gctgagcggc ttttaccttg
     1441 cttatttggc aggcttcccg ctccacagcc gctggatcgt ctggtccatc gtgctttacc
     1501 tggtcgcggg ggcgtgctgg ttgcctgtgg tctggatgca gatacgcatg cgcgacatgg
     1561 cggtacgcgc gacacagcag gaacaggctc tgccggccca atatgaacgt tatctgaagc
     1621 gctgggtgtt gcttggcatt ccggctttta cggccctggt cgtggtgttc tatttgatgg
     1681 ttgctaaacc ggtctagcgg tgcggtgacg gccgcgcgtg tcgctttcct gccttgcccg
     1741 gtcggcgata tccgcatact tttgcgccaa tcggttcaat tgctcatcgt ccaggttctc
     1801 cagcccgatc atcgcgttct gcgcgccttc caacgcacga atcagttcgt cgagcttgat
     1861 ttgcagggcc gtactgtccc ggttcaacgt gttttgaatc aggaacacca tcagcagcgt
     1921 gatgatggaa ctgcaagtgt tcatcatcag ctgccaggta tcgctgaagt ggaacaccgg
     1981 gccggtgatc acccatgtga ataccagcgc cacggcaata cagaatgcgg cgccgccgcc
     2041 gctcgcctgc gacacccact tggcaaatct ggagtatccc gaaaatcccg ccatctcact
     2101 gtgccgattg gtggactgca attccgaccg ccggctcatg tgttggttcg gcgcgcggtt
     2161 ggcggtgttg cgtaaacgcc acgcgcgacc ggtcgtgctt atgtgcaata ccgcacaaaa
     2221 ctgtgcgcta ccgcacagtt ccgagtcggc atgcgtagga cactgtagtc gttcgatgcc
     2281 gcaagcgctg gtgtgagcgg caaactagcc caagcaggcg caatgcgatg gctcggatca
     2341 agataattcg gtggcaacgc gcggagggtt tacctgacag cgatgtagga aaactcctac
     2401 gtcgaccaaa aaaatttctg acttcgcgcc cttgttcggc tgaaagaaac acgttactgt
     2461 ttttgaaatt acaaaaagct gaacaagtgc atgacatcac acaccgcacc ttgcgaagtt
     2521 cggcactttc caaagtcatg accgctcccg tcactcgcag catgcgtttg gcgaagcgga
     2581 cgttgcgtcc gtacgcaatc gagaacgcca tcggagagct tgagagatga gaaacgccca
//
LOCUS       DATMZQ010000376         6812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4097454, whole genome shotgun sequence.
ACCESSION   DATMZQ010000376 DATMZQ010000000
VERSION     DATMZQ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4097454"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN496_12125"
     CDS             <1..270
                     /locus_tag="VN496_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17748.1"
                     /translation="VSDPAFTTFTCRCDIHDIYGSHGSYGREPGYGLAPVSLWGIRAI
                     TGPDIHDIHDIHDIHGIHDIHGIHDIHGIHDSHDIHDIHGISNDS"
     gene            260..2248
                     /locus_tag="VN496_12130"
     CDS             260..2248
                     /locus_tag="VN496_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXF17749.1"
                     /translation="MIPELGHFSLIVALCIALTQAALPLLGAARGNTVWMSIARPAAQ
                     GQFVFVAFAFGCLAYAFVSNDFSVLNVATNSNSSLPWFYRFAATWGSHEGSLLLWVLM
                     LSVWMVAVSAFSRHLPSEMVARILGVMALISIGFLLFMLFTSNPFQRLLPAAADGNDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAFAFAIAALLGGKLDATWARWSRPWTTMAWIFL
                     TVGIALGSAWAYYELGWGGWWLWDPVENASFMPWLVGTALMHSLAVTEKRGGFRVWTV
                     LLAIIAFSLSLMGTFLVRSGVLTSVHAFATDPTRGIFILIFLVIVIGGSLALFAWRAP
                     KVGLGGSFEFVSRESALLANNVLLLVAAASVMLGTLYPLFLDALGLGKLSVGPPYFES
                     VFVPLMVPAVFLMGIGPIAKWKQAALPELATRLKWAFIIALVTALLLPFTLGKWTPMI
                     AFGLLLALWVILATLLNLKQRVAQLRRTSGLGALTQLRAIPLGYYGMLVAHLGVAVFI
                     IGVTLVKGYETEKDVRMQVGDTVEVGGYTFRLDGLAEVQGPNYEATRGSVAVLKNGRT
                     LRTLYPEKRFYRVQQMPMTEAAIDTGVARDLYVSLGEPVEGGAWVVRVYHKPFVDWIW
                     GGAFIMALGGILAISDRRYRLARSAGRDAAASAKIVTA"
     gene            2346..2867
                     /locus_tag="VN496_12135"
     CDS             2346..2867
                     /locus_tag="VN496_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HXF17750.1"
                     /translation="MKRFLPLLIFLVLVLFLAVGLSLNPREVPSPLIGKAAPAFRLPL
                     LQDPGRSFGTEDLKGKVWILNVWASWCTACLEEHPILVEFSKANVLPIYGLNYKDKHE
                     DALKWLSEHGNPYAMSIEDGDGRVGIDYGVYGVPETYLIDGNGVIRYKRVGPVTPEIL
                     QNKILPMAKQFRG"
     gene            2864..3328
                     /locus_tag="VN496_12140"
     CDS             2864..3328
                     /locus_tag="VN496_12140"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HXF17751.1"
                     /translation="MKIAGALLLLMLYGIGLAREAPSAADDPALENRVMKLTSDLRCL
                     VCQNQSLADSHADLAIDLKNQVRSQMRAGKSDAEIRNYMVARYGDFVLYDPPFKASTL
                     LLWAGPFALLVLGLFVLAVYLRRRRRLVETELTPSEHARAQALLHGESEKSQ"
     gene            3325..4539
                     /gene="ccmI"
                     /locus_tag="VN496_12145"
     CDS             3325..4539
                     /gene="ccmI"
                     /locus_tag="VN496_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HXF17752.1"
                     /translation="MIAFAVTAFFLLIAALLIVLWPLLRRSTSPVLTPERSNLDIHRI
                     QLAELKNDLEAGTLASDRYQQAMTELERRVLEDSGAKAITPRSLSSGKTVPMIVGLFI
                     PVTAIALYLYLGNLQGIGAPRHPAADLSSVTAEQFQDMTAKLAARLQQNPNDAEGWKM
                     LGRAYRAMERYAEASQAYKRAVALAPENADLLADYAESLALQAGRSLAGEPTRLLDRA
                     LKLDPANAKVLTLSGSAAFERNDYKTAIQRWEAVLKQPGLGGELTQALQKGIEESKQR
                     MSGKPVTAETAGKERVTGVVRLDGALNGKVQPDDTVFVFARAASGPRMPLAIVKIKVA
                     DLPYKFSFDDSSAMTQAMKLSRFNDVVIGARISKSGSATPSAGDLEGTSGKVKAGSDG
                     IQVLIDRPVAGS"
     gene            4536..5558
                     /gene="mltB"
                     /locus_tag="VN496_12150"
     CDS             4536..5558
                     /gene="mltB"
                     /locus_tag="VN496_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HXF17753.1"
                     /translation="MTRRLLLACLLFMGGTFATLAEAPSKIRLKPEVEKFITQMVQQH
                     EFNKSYLQQLFAQLKSNDGVVKAINAPATAKPWYEFKGIFITPTRTTAGVAFWNDNVE
                     QLKRARDIYGVPEEIIVAIVGVETIYGKRTGSFKVIDALYTLGFEMPERATYFRSEME
                     QFLLLTRENGLDPLAVKGSFAGAIGMPQFMPTSYRRFAVDFDSDGKIDLWNSVPDVIG
                     SVANYLHFFGWTAGQPVAVPARLSGNDYAGIVEKGFKPHLTLAQMLPKGVEATEDVSG
                     ELIAGLFGLELEQGQEYWLAFSNFYAITRYNRSKNYAMAVHLLAQAIARERELQNAPP
                     VSAAKS"
     gene            5586..6191
                     /locus_tag="VN496_12155"
     CDS             5586..6191
                     /locus_tag="VN496_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HXF17754.1"
                     /translation="MPKSEYSTLDHLLTAVRQCRACADQLPFGPRPVLRAAPSARMLI
                     VGQAPGLRVHRSGIPWDDASGKTLRTWIGIGQEQFYDESRIAIIPIGYCYPGRGKGGD
                     LPPRRECAQLWLTQLLAKLPHIELTLLIGLHAQRRFLAKRRKATLTQTVRAWREYQPA
                     YLPLPHPSARNTPWFQRNPWFKAELLPALRKRWNQNCGDLT"
     gene            complement(6207..>6812)
                     /locus_tag="VN496_12160"
     CDS             complement(6207..>6812)
                     /locus_tag="VN496_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448512.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXF17755.1"
                     /translation="QIFTRIGASDDLAGGRSTFMVEMTEAANILHNATNLSLVLMDEV
                     GRGTSTFDGMALAMAIARHLVQATRSYTLFATHYFELTRLADEYREVANVHLDAVEHK
                     DTIVFLHAVAEGPASQSYGIQVAQLAGLPAAVVRAARTQLARLDEEAASRAPQGDLFS
                     VPAPKESPPHPALEALKQMDPDALSPKEALELIYRLKKIAD"
BASE COUNT         1324 a         1882 c         2081 g         1525 t
ORIGIN      
        1 gtaagcgatc cggcgtttac gacattcacg tgtcgttgcg atattcacga tatttacggc
       61 agtcacggca gttatgggcg agagcctggt tatggactcg ccccggtatc cctttgggga
      121 atccgggcga taaccggacc cgatattcac gatattcacg atattcacga tattcacggc
      181 attcacgata ttcacggcat tcacgacatt cacggcattc acgacagtca cgatattcac
      241 gacattcacg gcatttcaaa tgattcctga actggggcat ttctcgctga tcgtcgcgct
      301 ctgcatcgcg ctcacgcagg cggcacttcc gctgctcggc gccgcccgcg gcaacacagt
      361 ctggatgagc attgcgcggc cggcggcgca gggccaattc gtgttcgtcg cgtttgcgtt
      421 cggctgcctg gcatatgcgt tcgtcagcaa tgatttctca gttctgaatg ttgctacgaa
      481 ttccaattcc agcctgccgt ggttttaccg ttttgccgcg acgtggggca gccacgaagg
      541 atcattgctg ctgtgggtac tcatgctttc cgtctggatg gttgctgtca gcgccttcag
      601 ccggcatctt ccgtcggaaa tggttgcgcg cattctgggt gtgatggctc tgatcagcat
      661 cggattcctg ctgttcatgc tgttcacatc gaatccattc cagcgtttat tgccagcagc
      721 tgccgacggc aacgatctga acccgctgtt gcaggacccg ggcatggtga ttcatccgcc
      781 gatgctgtac atgggatacg tcggattttc ggtggcattc gcctttgcga tcgcggcgtt
      841 gctcggcggc aagcttgatg cgacgtgggc gcgttggtcg cgtccatgga caaccatggc
      901 gtggatcttt ctgacagtgg gtatcgcgct cggcagcgcc tgggcctact acgagctcgg
      961 gtggggtggc tggtggttat gggatccggt tgaaaatgca tccttcatgc cctggctggt
     1021 gggcacggcg ctgatgcact cgttggcggt gaccgaaaag cggggcggtt tcagggtgtg
     1081 gaccgtgctg ctggccatca ttgcattctc actatcgctg atgggaacct tcctggttcg
     1141 ctctggcgtg ctgacttcgg tgcacgcgtt cgcaaccgat ccgacgcgcg gcatattcat
     1201 tctgatattt ttggtcatcg ttatcggcgg ctcgctggcg ctgttcgcgt ggcgggcacc
     1261 gaaagtggga ctgggcggga gtttcgagtt cgtgtcgcgc gaatcggctt tgctcgccaa
     1321 caatgtgctg ctgctggtcg ccgcggcatc cgtcatgttg ggaacgttgt atccgctttt
     1381 cctggacgcg ctcggactgg gaaaattgtc tgtcggaccc ccttatttcg agtcggtatt
     1441 cgtgccgctg atggttccgg ctgttttttt gatgggtatc ggcccgattg caaaatggaa
     1501 gcaggcggct ttgccggaat tggccacgcg cctgaaatgg gcgttcatca ttgcgctggt
     1561 taccgcgctg ctgttgccat ttacgttggg caaatggacg ccgatgatcg ctttcggcct
     1621 gctgctcgcc ttgtgggtga tactggcgac tttgctaaac ctcaagcaac gggttgcgca
     1681 gttgcgccgc accagcgggc tcggggcctt aacgcagctg cgggcgattc cacttggcta
     1741 ttacggcatg ttggtggccc acctcggtgt cgcggtcttc atcatcggcg tcacgttggt
     1801 aaaaggctac gagaccgaaa aagatgtgcg catgcaagtt ggtgataccg tggaggtcgg
     1861 cggctacact ttccggttgg acgggctggc ggaagtgcaa ggtccgaatt atgaggcgac
     1921 gcgcggttcg gtggccgtgc tgaagaacgg taggacgctg cgcaccctgt atcctgaaaa
     1981 gcgcttctat cgggttcagc agatgccgat gactgaagct gccatcgata ccggcgtagc
     2041 gcgcgatctg tatgtttccc ttggtgagcc ggtcgaaggc ggggcgtggg tcgtgcgcgt
     2101 gtatcacaag ccgttcgtgg attggatctg gggcggtgcc tttatcatgg cgcttggcgg
     2161 cattctcgct atcagtgacc ggcgttaccg cctcgcgcgc agcgcagggc gtgatgccgc
     2221 cgcaagcgcg aaaatcgtaa ccgcgtgatc agcgcacctg ttgtgatcag cctgaaagct
     2281 acgggtcaaa tctttcctgc cttgccgccg cggcatttgt atcgacagga ccattactct
     2341 cttccatgaa acgttttctg ccgctgctca tcttcctggt gctcgtcctg tttcttgcgg
     2401 taggcttaag cttgaatcca cgggaagtgc cttccccgct gattggcaag gcagcgcctg
     2461 ccttccgcct gccgctgttg caggaccccg gccgcagctt tggcacggaa gatctcaagg
     2521 gcaaagtttg gatactcaat gtctgggcgt cctggtgcac ggcctgtctg gaggagcatc
     2581 cgattctggt agagttttcg aaagccaatg tgctgcccat ctatggcctc aactacaaag
     2641 ataaacacga agatgcattg aagtggctaa gtgaacacgg caacccgtat gccatgtcca
     2701 tagaagacgg cgacgggcgt gttggcattg actacggcgt atatggcgtg ccggaaacct
     2761 atctcatcga cggcaatggc gtgattcgct ataagcgcgt cggtccggtc accccagaaa
     2821 tactccagaa caaaatcctg cctatggcga agcaattccg cggatgaaaa tcgctggcgc
     2881 gttgctgttg ctgatgctgt atggaattgg tctggcccgc gaagcaccca gcgcagccga
     2941 tgatcccgcg ctggaaaacc gcgtgatgaa gttgacgtcc gatctgcggt gcctggtttg
     3001 ccaaaaccag tccctggccg attcgcatgc cgatctcgcc atcgatctga aaaaccaggt
     3061 gcgcagtcag atgcgggccg gcaagagcga cgccgaaatt cgcaattaca tggtggcgcg
     3121 ttacggggat ttcgtactct acgacccgcc atttaaggcg agcacgctat tgctgtgggc
     3181 ggggcccttc gcgctgctgg ttttgggcct gtttgtgctt gcggtctact tgcgccggcg
     3241 ccgcaggctg gtcgagacgg aattgacgcc gagcgagcac gcgcgcgcgc aagccctgct
     3301 gcatggcgag tccgaaaaat cgcaatgatt gcattcgctg tcaccgcgtt tttcctgttg
     3361 attgccgcgc tgctcattgt gctatggcct ctgctgaggc gctcgacgtc gcctgttctg
     3421 acaccggaac gttccaatct cgacattcac cgcattcagc ttgccgagct caaaaatgac
     3481 cttgaagcgg gcacattggc gtcggatcgt taccagcaag ccatgactga actggagcgc
     3541 cgcgtgctgg aggacagtgg cgccaaggcg attacaccgc gatccttgtc ctcgggcaaa
     3601 accgtgccga tgatcgtcgg cctgtttatt cccgtaaccg ccattgcgtt gtacctgtat
     3661 ctcggtaatc tgcaaggcat cggcgcgcca cggcatcctg ccgcggatct gtcctcggtt
     3721 accgcagaac aatttcagga catgacggcc aaactggcgg cgcggctgca acagaacccg
     3781 aatgatgcag aaggttggaa aatgcttgga cgcgcttacc gggccatgga gcgctatgcc
     3841 gaggccagcc aagcgtacaa acgggcagtc gcgctggcgc cggaaaatgc cgatctgctg
     3901 gctgactatg cggagtctct ggcgttgcag gccggacgat cgttggcggg tgagccgacg
     3961 cgattgctgg atcgcgcgct gaagctcgac cctgccaacg caaaagtgct gactttgtcg
     4021 ggcagcgcgg cgttcgagcg caacgactac aaaactgcca tccagcgctg ggaggcggtt
     4081 ctgaaacaac cgggtctggg aggtgaattg acgcaggcgc tacagaaagg gatcgaggaa
     4141 tcgaagcaac gcatgtcggg caagccggtc acggccgaga cggctgggaa agagcgtgtc
     4201 accggtgtgg tgcgactcga tggcgcgctg aatggtaaag tgcagccaga cgatactgta
     4261 ttcgtttttg cgcgtgccgc atcggggccg cgcatgccgc tggcgatcgt caagatcaaa
     4321 gtggcagacc tgccctataa attttctttt gacgactcct cagcaatgac ccaagccatg
     4381 aaattatccc gcttcaacga cgtagtgatc ggcgcgcgca tctcgaagtc gggcagtgcg
     4441 acgccttccg cgggtgatct ggaaggaacg agcggcaagg tcaaagccgg cagtgacggg
     4501 attcaagtgc tgatcgatcg tccggtcgcc ggatcatgac gcgccgtctt ctactggcct
     4561 gcctgttgtt catgggggga acctttgcga cgctggccga ggcgccgagc aagatcaggc
     4621 tcaagccaga ggtggagaaa ttcataaccc agatggtgca gcagcacgaa ttcaacaagt
     4681 cgtatttgca acagctattt gcgcaactga aaagtaacga cggcgtggtg aaggcgatca
     4741 atgcgccggc taccgccaaa ccctggtacg aattcaaagg cattttcatc acgccaaccc
     4801 gtaccaccgc cggtgtggcc ttctggaatg acaatgttga gcaattgaaa cgcgcgcgcg
     4861 atatctatgg cgtgccggaa gaaattattg ttgcgatcgt tggcgtggaa accatctatg
     4921 gcaagcgcac gggttcgttc aaggttatcg acgcgctgta cacgctgggt ttcgaaatgc
     4981 cggaacgcgc gacctacttc cgcagcgaga tggaacagtt tctgctcctt acgcgggaga
     5041 acgggctgga tccattagcg gtgaaaggat catttgccgg cgccatcggc atgccgcagt
     5101 tcatgccaac cagttatcgg cgttttgcag tcgacttcga cagcgacggc aagatcgact
     5161 tatggaacag cgttcccgat gtcattggca gcgtcgcaaa ttacctgcat ttcttcggtt
     5221 ggaccgcagg acagccggta gcggtgccgg cgcggctcag cggcaatgac tacgcgggta
     5281 ttgttgaaaa gggattcaaa ccgcatctga cgcttgcgca gatgctgccg aaaggcgtgg
     5341 aggcaacgga ggacgtgtcc ggcgaattga tcgccggact atttggcctg gaactcgagc
     5401 aagggcagga atactggctg gcattcagta atttctatgc gatcacgcgg tacaaccgca
     5461 gtaaaaatta tgcgatggcg gtccatctgc tggcgcaggc aatcgcgcgc gagcgcgaat
     5521 tgcagaatgc gccgccggtg tcggcggcga aatcctgagc gtctttcatc gggaggcgat
     5581 gattgctgcc caaaagcgaa tattccaccc tggatcattt gctgacggca gtccgtcaat
     5641 gccgcgcgtg cgcggaccag ttgcctttcg ggccacgccc ggtgttgcgc gccgccccat
     5701 ccgcgcgcat gctgatcgtg gggcaagcgc ctggcctgcg cgtgcatcgc agcggaattc
     5761 cgtgggatga cgccagtggt aaaacgctgc ggacgtggat cggtattggg caagaacagt
     5821 tttacgatga atccagaatc gccatcatcc ccattggcta ttgctatccg ggaaggggta
     5881 agggcggtga tttgccgccg cgcagggaat gcgcacagtt gtggctgacc caattgctgg
     5941 cgaagcttcc acatattgag ttgacgctgc tgattggctt gcatgcgcag cggcgcttcc
     6001 ttgcaaagcg acgcaaggca acgctaacgc agacggttcg ggcctggcgc gaatatcagc
     6061 ccgcttactt gccgttgcca cacccttcag cccgcaacac cccgtggttt caacgcaacc
     6121 cgtggttcaa agccgaactg ctgccggcat tgcgcaagcg gtggaatcag aattgcggtg
     6181 acttaacttg acggcggccc gtacattcag tccgcgattt tcttcagtcg ataaatcagt
     6241 tcaagtgctt ccttgggtga tagcgcatcg ggatccattt gctttagcgc ttcgagtgcc
     6301 gggtgcggcg gcgattcctt tggtgccggc acgctaaata aatcgccctg cggggcgcgg
     6361 ctggcggctt cttcgtcgag tcgcgccagt tgcgttctgg ccgcacgcac aaccgcagca
     6421 gggaggccag ctagctgagc gacctggatg ccgtaacttt gagaagccgg gccttccgca
     6481 acggcatgca ggaaaacaat ggtgtctttg tgctctactg cgtccagatg cacgttggcc
     6541 acctcacggt attcatcggc caggcgcgtc agctcaaagt aatgggtagc aaacagcgtg
     6601 taactgcggg tagcttgcac cagatgccgg gcaatggcca ttgccagggc catgccgtcg
     6661 aacgtcgagg tccctcggcc cacttcgtcc atcagcacca acgacagatt ggtcgcattg
     6721 tgcagaatgt ttgcggcctc cgtcatttcc accatgaatg tcgagcgtcc gcccgccaga
     6781 tcatcggaag cgccgatgcg cgtgaagatc tg
//
LOCUS       DATMZQ010000377         3183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1533195, whole genome shotgun sequence.
ACCESSION   DATMZQ010000377 DATMZQ010000000
VERSION     DATMZQ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533195"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VN496_12165"
     CDS             <1..1007
                     /locus_tag="VN496_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HXF17756.1"
                     /translation="QLRSALTLIDDDVARLSDALAHLALQHGKTSMPGRTWLQHAVPI
                     TFGLKIAGALSAVERHRMRLRELRARVLVIQFGGAAGTLASLGARGVEVCNALATELK
                     LANPDMPWHAHRDRVAEVATALGLLTGTLGKIAKDISLLMQTETAEVTAPDTPGKGGS
                     SSMPQKRNPVDAASVLSAAIRVPALVSVMLSAMVQEHERGLGNWHAEWETLPEICMLC
                     SGALAHVIQVIEGLQVNAQRMRQNLHETGGLIMAEAASAALARKIGRAAAHDLIEAAS
                     RRALEQKASLLEVLMDDAEVRQHFSAGELERLLDASGYLGAAEAFVGRVLAARSGEHK
                     "
     gene            1079..2221
                     /locus_tag="VN496_12170"
     CDS             1079..2221
                     /locus_tag="VN496_12170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXF17757.1"
                     /translation="MKIANIADLRELAWRRLPRVLFDYIDGGAQDEVTLRANQEDFGR
                     ITLVQRVLRDVSKRDHSVTVLGQKYDLPLILSPTGMTGLFWPNGALEAARAASAAGAG
                     FCLSSMSTSTVEDIAKVVKMPSWFQLYVMRDRGMSRELIERAKAAGCSALVLTVDLAL
                     QGQRDRDVHNGLTIPPTLRISNLLNFTKRPGWVWRYLMGPKVLPASLVKPGQNTLFTI
                     AGYVNSQFDQSVTWEDIEWAKSIWGGPLAIKGILDPSDAKLAVEHGVDAVIVSNHGGR
                     QLDSVRSAISALPDVVDAVAGRAEVLLDGGVRRGTDVLKAIALGAKACMIGRPYMYGL
                     AAKGRPGVTQALELLRNEIDLALALTGNTSLSSLDASSILQARNAM"
     gene            complement(2239..>3183)
                     /locus_tag="VN496_12175"
     CDS             complement(2239..>3183)
                     /locus_tag="VN496_12175"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXF17758.1"
                     /translation="QPLGITLTPDGKKLYVSNNFANSVSVVDANSNQPITTVATERGP
                     VGLAASPDNTRVYVTNKDSGSVSVIDVASDTILGNPITVDSQPVSIAISPDGQRAYVA
                     NQGSNFVTEIGSTRTLTIAKAGTGFGTVTSLPAGILCGASCQARFAANTAVQLAAVAG
                     TNSEFSGWSADCVNGNVTLNVSRTCTVIFTSVVQPGSGGNSSGGGSSGGSSDCFIATA
                     AYGTAMAEEVMTLRRFRDDYLIRSGAGREFVQLYYRFSPPVAAYIRDRDSLRAATRIA
                     LWPVVVVIKQPGAVGLPIALVWLVFRIKKRLSERADVR"
BASE COUNT          636 a          927 c          989 g          631 t
ORIGIN      
        1 tgcagttgcg cagcgcgctg acgctgatcg atgacgacgt cgcgcgcctg tcggatgcgt
       61 tagcccatct cgcgcttcaa cacggcaaga cttcgatgcc tggacgcacc tggctccagc
      121 acgcggtacc gattaccttc gggctgaaaa tcgcgggcgc gttgagcgcg gtggaacgac
      181 atcgaatgcg gctgcgtgag ctgcgcgccc gcgttctggt gatccagttc ggaggcgccg
      241 ccggaacgct ggcttccctc ggtgcgcggg gggtcgaggt ttgcaatgca ctggccaccg
      301 aactgaagct ggcgaatccg gacatgccgt ggcatgcgca tcgtgatcgt gtcgcggaag
      361 tcgcgacggc cctcggcttg ctgacgggca cgctcggcaa gattgcgaag gacatttcgt
      421 tgctcatgca gaccgagacc gccgaagtca cggcgcctga cacgccaggc aagggcggtt
      481 cttcgagcat gccgcagaag cgcaatccgg tcgatgccgc gagcgtgctg agcgcggcca
      541 tcagagtgcc ggcgctggta tcggtgatgt tgtcagccat ggtgcaggaa cacgaacgtg
      601 gtctgggaaa ctggcacgcg gaatgggaga cactgcccga aatctgcatg ctttgttccg
      661 gtgcgttggc gcatgtgatc caggtgattg aagggctgca ggtcaacgcg caacgcatgc
      721 gtcagaacct gcatgaaacc ggcggcttga tcatggccga agcagcatcc gccgccctgg
      781 cacggaagat cggccgtgca gccgcacacg acctcattga ggcggcatcg cgacgtgcgc
      841 tcgaacagaa agcctcttta ctcgaagtgc tgatggacga cgcggaagtg cgccagcatt
      901 tttccgcagg cgaactggaa cgcttgctcg atgcttccgg ctatctgggc gcagccgaag
      961 cattcgtcgg ccgggtgctg gccgcacgtt ccggagagca caaataacta atggacgcac
     1021 gggagagtgc tgacaaatcg taacctcggc catcagtcca taacaagaaa gacatcgcat
     1081 gaaaatcgca aacatcgcgg acctgcgtga gttggcgtgg cgccgtctgc cgcgcgtgct
     1141 gttcgactat atcgacggcg gagcgcagga cgaagtcacg ctgcgtgcca accaggaaga
     1201 tttcggacgc atcacgcttg tgcagcgcgt gctgcgggac gtatcgaagc gcgatcattc
     1261 ggtcaccgta ctcggccaga agtacgattt gccgctgata ctttctccca ccgggatgac
     1321 cgggctgttc tggcccaacg gcgcattgga ggcagcgcgc gcggcaagcg ctgccggtgc
     1381 gggattctgt ttgagttcga tgtccacctc cactgtcgaa gacattgcca aggtggtcaa
     1441 gatgccaagc tggttccagc tctatgtcat gcgcgatcga ggcatgtcac gcgagttgat
     1501 cgagcgcgcc aaggccgctg gctgttcggc tctggtgttg acggtagatc tggcgttgca
     1561 aggccagcgc gatcgcgacg tgcataatgg cctgacgatt ccgcccacgc tgcgtatctc
     1621 aaacctgctg aatttcacaa agcgtccggg ctgggtgtgg cgctatctga tgggcccgaa
     1681 agttctgccg gcgagcctgg tcaagcccgg acagaacacg ctgttcacca tcgccgggta
     1741 tgtcaactcg cagttcgatc agtcggtgac gtgggaggac atcgaatggg cgaaatccat
     1801 ctggggcggg ccgctcgcta tcaaaggcat actggatccc tccgatgcaa agcttgctgt
     1861 cgagcacggc gtcgacgctg tcatcgtatc gaaccatggt ggaagacagc tggacagcgt
     1921 gcgctccgcg atttcggcac tgccggacgt ggtggatgcc gtggcgggcc gcgcagaagt
     1981 gttgctggat ggcggcgtgc gccgcggtac cgatgtacta aaggccatcg cgctgggtgc
     2041 gaaagcctgt atgatcggca ggccttatat gtatggactc gctgccaaag gccggccggg
     2101 ggtgacccag gcgttggaac tgctgcgcaa cgaaattgat ctggcgctgg ccttgactgg
     2161 caacacctcg ttgtcgagcc tcgacgcatc ttcgattctc caagcacgaa acgcaatgta
     2221 gtcgccagtg ccgtgaaatt aacgaacatc ggctcgctca gacagcctct tcttgatacg
     2281 gaagaccagc cacactaatg cgattggcaa gccgactgcc ccgggttgct tgatgacaac
     2341 gacgactggc caaagagcta ttctggtcgc ggcgcgcaac gaatcgcgat cgcgtatgta
     2401 ggccgcgaca ggaggagaaa agcggtaata caactggacg aattcgcgtc cggcgccgct
     2461 cctgatcaga tagtcgtcac ggaagcggcg caatgtcatc acttcttcgg ccatagcagt
     2521 gccgtatgcg gccgtcgcga taaagcagtc gctgctacca ccactgctcc cgccgccgct
     2581 gctatttccc ccgctgcccg gttgcaccac cgaagtaaag attaccgtgc aggtccggct
     2641 gacattcagc gtaacgttgc cattcacaca atccgcgctc catcccgaga actctgaatt
     2701 ggtccccgcg actgccgcta actgcaccgc agtattcgcc gcaaatcggg cctgacaaga
     2761 agcgccgcat aggatacccg ctggcaagga agtaactgtg ccgaagccgg tgccggcctt
     2821 cgcaatggtc aatgtcctcg tgctgccgat ctcagtgaca aagttgctgc cttgattggc
     2881 gacatacgcg cgttgaccgt ccggactgat ggcaatgctc acaggttggc tgtcgacggt
     2941 tatcgggttg ccgagaatcg tatcgcttgc cacatcgatt acgcttaccg aaccgctgtc
     3001 tttgttcgtg acatataccc gggtattgtc cgggcttgcc gccagcccga cggggccacg
     3061 ttccgttgcg accgtcgtta taggctggtt cgaattcgca tcgactacgc tcactgagtt
     3121 cgcaaaatta ttggacacgt agagtttttt gccgtccggg gtcaatgtga taccgagcgg
     3181 ctg
//
LOCUS       DATMZQ010000378         4942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2716141, whole genome shotgun sequence.
ACCESSION   DATMZQ010000378 DATMZQ010000000
VERSION     DATMZQ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716141"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..795)
                     /locus_tag="VN496_12180"
     CDS             complement(217..795)
                     /locus_tag="VN496_12180"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HXF17759.1"
                     /translation="MRTPEEIEAEIDRTRDRMDATLTELEFRLTPRELIRDGINSLSG
                     LQASRYVGKYAGKMMHLGRRYPKPAAIAGASLAGLYLAGRRFKNRTSEDQTSAARLSQ
                     AMDVARETMQDTKKTLAKTAGAAKDKVVGATSDAMERASDIADSASKRLRQAGTSVQS
                     VTRERPVAAGAVALALAAAVLMSIPSVRRRLY"
     gene            complement(806..1228)
                     /locus_tag="VN496_12185"
     CDS             complement(806..1228)
                     /locus_tag="VN496_12185"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXF17760.1"
                     /translation="MESLGSTTREYHRRPLVGLISDLWRESATLLRDEAELAKSEVSE
                     KMSELGSGIGSLALAGAVLFGGFLLILFAAVGLIAKVLPDEQAQWLAPLLVGVVVLFI
                     GWMLLSAGRNKLKGGNLTPSRTVRSVQRNAEVVKEHIR"
     gene            complement(1557..1736)
                     /locus_tag="VN496_12190"
     CDS             complement(1557..1736)
                     /locus_tag="VN496_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17761.1"
                     /translation="MKSLAQAWQRKRRLFLSGFCTTHALTWCHPRSAAGYRQSLDHAF
                     GQEELIVRAYACRRL"
     gene            1737..2372
                     /locus_tag="VN496_12195"
     CDS             1737..2372
                     /locus_tag="VN496_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXF17762.1"
                     /translation="MARASDKPRKHFSMIRGMHLADLFTLANAACGVAGVFLAMAYVG
                     SHSLAHFFAAACLTPLAFGFDVIDGRIARWRHTHSALGRELDSLADVISFGVAPAALG
                     YAAGLQGAWDWAALVYFVCCGVSRLARYNVTAEQLSGTAGKVAYFEGTPIPTSIVLTG
                     VLALAAWNDRLGQNILWGVWSLGGAELHPLSLLFVVSGTLMISKTLHIPKL"
     gene            2576..3511
                     /locus_tag="VN496_12200"
     CDS             2576..3511
                     /locus_tag="VN496_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17763.1"
                     /translation="MQQRTNVGTEARKPIPPSNEGGWKEPQPPFPEQHQDSPGLESKL
                     EPLPRYQAEDYRAAGKLKGKVALITGGDSGIGRAVAVLFAREGATVAINYLPQEETDA
                     QTTRSAVEAYGGECMLVAGDLTQKEFCVRLVEETVRRFGKIDILVSNAAHQQRKDDLA
                     QLTDDELETTFTTNIYAYIRLARAALAHMKPGGVIIATSSETGLLGSEKLPDYSATKG
                     AINAFTKALAQALIQRQIRVNAVAPGPVWTPLNPSDAGLAAQNVAKFGQKNPMGRPAQ
                     PEELAPAYVFLASNADSSYITGTVLQVMGGETTGG"
     gene            3973..4398
                     /locus_tag="VN496_12205"
     CDS             3973..4398
                     /locus_tag="VN496_12205"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXF17764.1"
                     /translation="MVKRAWAVWKGGIKDGGGTISTETGVLKEAPYGFKSRFENGKGT
                     NPEELIGAAHAGCFSMALSLMLGTAGMTPEKIETQAEVSLDKAGEGFEITASHLTVRA
                     KIPGANPAQFQEIANKAKAGCPVSKVLKAKITMDARLET"
     gene            complement(4409..>4942)
                     /locus_tag="VN496_12210"
     CDS             complement(4409..>4942)
                     /locus_tag="VN496_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF17765.1"
                     /translation="LTEMHGGTVAATSSGEGQGATFTVKLPVGAPLGSGQGAVPAIAA
                     RTRSDVPPRKLRGVKVLVVDDEPDAREMIKRFLDACEAEPMLAGSADEALELMERFRP
                     DVIVSDIGMPKKDGYSFMRSIRHRGINIPAVALTAFARAEDRVRSIQAGYQTHLPKPF
                     EPGELLALIASLSGRYE"
BASE COUNT         1008 a         1435 c         1471 g         1028 t
ORIGIN      
        1 ctgttcgatg ccagcgcgat gttcgccgga cagtgcgagc gacagatcga tatcgccaat
       61 ctcagcagcg aaggtgtcgt taacggggaa aactaccatg gtcgtccgcc attgccgaaa
      121 gccttcacgc tatagcggta accgatatct tgtcaattgc gcactgcgcc ggccgtcctg
      181 tggcgaactg gcagtgcgcc ctcaaaccat tggaagtcag tagagccttc tgcgcacgga
      241 agggatgctc atgagcacgg cagccgccaa ggctagcgcg acggcgcctg cggcgacggg
      301 ccgctcgcgc gtgacgctct gcacactggt gcctgcttga cgtaaccgct tgcttgcgct
      361 gtctgcaata tcgcttgctc gctccattgc atcggacgtt gcaccaacaa ctttgtcctt
      421 cgcggcaccg gcagttttgg cgagcgtttt cttggtgtcc tgcatcgtct cccgggcgac
      481 atccatcgcc tgggacaggc gggcagcgga agtttgatct tccgatgttc ggtttttgaa
      541 gcggcgtccc gccaaataaa gaccggcgag gctggcgccg gcgatggctg cgggcttcgg
      601 atagcgccgg ccaagatgca tcattttccc ggcatatttg ccaacatacc ggcttgcctg
      661 gagcccggac agggaattta ttccgtcgcg aatgagttcg cgtggtgtaa gacgaaactc
      721 gagctcggtc agcgttgcat ccatccgatc gcgggtgcgg tctatctcag cttctatttc
      781 ttcgggcgtt ctcatttttg gatccttatc tgatgtgctc tttgacgacc tcggcattac
      841 gttgtaccga gcggacggtc cgggatggag tcagattgcc acccttcaac ttgttccgac
      901 cggcggatag cagcatccag ccaatgaaca gaacaacgac gcccaccagc aacggtgcca
      961 gccactgggc ttgctcgtcg ggcagcacct ttgcaatcaa acctacggcg gcaaacaaaa
     1021 tcaaaagaaa gcccccaaac aatacggcgc cggctaatgc aagggagccg atgccgctcc
     1081 ctagctcgga cattttttcc gaaacttctg acttcgccag ttctgcctcg tcccgtagca
     1141 gcgtagccga ctcgcgccac aaatctgaaa tgagcccgac caatgggcgc ctgtggtact
     1201 cccgggttgt tgaccccaaa ctttccatcg ctcgctcctt cgcaattcgg tcacgtctcc
     1261 aatgatgccg tgcggcgcgc gcagcacggg ataccagtcc gaggcaatat gcgtgccgaa
     1321 ttttatacgc tgctcgcggg cgccagtcgt ggcgttttga cgcgcgaaat gccggtcagc
     1381 caatggctta ttgcattatc ggcacgcaaa agtgcccgat gtctatggtg cttcacggca
     1441 cttgggccgc ccggatggca gactttggca gcggctcgtg taatttttgc cgccgactgg
     1501 ctgaaccgca cacacgacag tgctctattg caagcccgat gtataaagac tggtctttaa
     1561 agccggcggc atgcgtaggc tcggacgatg agctcttcct gtccgaacgc atggtccagg
     1621 ctctgccggt agccggctgc actgcgcgga tgacaccagg tgagcgcgtg cgtggtgcag
     1681 aaaccggaca gaaacaagcg gcgcttgcgc tgccaggctt gggctagact cttcatatgg
     1741 cacgcgcaag cgacaagccg cgcaaacatt tcagcatgat ccgcggcatg cacctcgccg
     1801 atctctttac gctcgccaat gctgcctgtg gcgtggctgg agtgttcctg gcgatggcct
     1861 atgtcggcag tcactcgctg gcgcattttt ttgccgcggc atgtctcacg ccgctagcat
     1921 tcggcttcga tgttatcgat ggccgcattg cacgctggcg gcatacgcat tctgcgctgg
     1981 ggcgcgaact ggattcgctg gcggatgtga tttcatttgg cgttgccccg gctgcgctcg
     2041 gttacgcggc gggactccag ggtgcatggg actgggcggc gctggtctac ttcgtatgct
     2101 gcggagtaag ccgactggcg cgctataacg tgaccgcgga acaactttcg ggaaccgctg
     2161 gcaaagtcgc ctacttcgaa gggactccca ttcctaccag cattgtcttg actggcgtgt
     2221 tggcgctggc cgcctggaat gatcgccttg ggcaaaacat tttgtggggc gtctggtcgc
     2281 ttggaggagc ggaactgcat ccgctgtcct tgttgtttgt cgtatcggga acgctgatga
     2341 tcagcaagac cttgcatatt ccgaaactct gacgatgcgc gtcccgacgc cctgaaaact
     2401 cggatttccg gtaattcata ccggcctttg taaagttgca tgagacaaag cgccgccagt
     2461 gtgccgctga cgccgatctc ccgcgcgcag ccgcagcgct gttctggcaa cgttcttgct
     2521 gaccgctgtg caaacaccgc gcaatgtcat tcggcgcata tcagaggaga acgtcatgca
     2581 gcaacggaca aatgtcggca ctgaagcaag aaagcctatc ccaccttcca acgagggtgg
     2641 ctggaaagaa ccccaacctc ctttcccgga gcagcatcag gacagtccgg gactggagtc
     2701 gaagctcgag ccactgccgc gctatcaggc ggaagactac cgggcggccg ggaagctgaa
     2761 aggaaaagtc gcactcatta ccggtggtga ttccggaatc ggtcgggcag tggcggtgtt
     2821 gttcgcgcgc gagggcgcga cggttgccat caattatctt ccccaggaag aaacagacgc
     2881 gcagaccacc cggtctgcag tcgaggcgta cgggggcgaa tgcatgctag tcgcaggcga
     2941 tcttacacag aaagaattct gtgtgcggct cgtggaggaa acggtccggc ggtttggcaa
     3001 gatcgacatt ctggtgtcga acgctgcaca ccagcagcgc aaggatgatc ttgcgcaatt
     3061 gacggacgac gaacttgaga cgacgttcac tacgaacatc tatgcgtata tccggctggc
     3121 gcgcgcagcg ctggcccata tgaagcccgg tggcgtcatc attgcgacga gctcagagac
     3181 gggactgctg gggtccgaga aactccccga ctattctgcg accaagggcg cgatcaacgc
     3241 gttcaccaag gcccttgcac aagcgctgat tcagcggcag attcgcgtca acgccgttgc
     3301 accgggcccg gtatggacgc ctcttaatcc ctcggatgcc ggcctggctg cgcagaatgt
     3361 cgcaaaattt ggccagaaaa atccgatggg ccggcccgcg cagcccgagg aactggcacc
     3421 ggcatatgtc ttcctggctt ccaatgcgga ttcttcctac atcacgggaa cggtattgca
     3481 agtgatgggg ggtgaaacga cgggtggatg atgggcagcg ttcaccgctc gagcgttcga
     3541 cgcgtgcgat gcaaggccag atggtggtgc ccatgaaagt aaaacgcccc gtcgcttgcg
     3601 cgattggggg cgtttttttg ccaggcgttg ccggcctaac tgcttgccag actcctgaac
     3661 gttgcggtag atccagttcc agagtactgc cggccggtgc tgccttgtag gtctctcacg
     3721 ggatcacctc ctttcaaagt tgcgaggcgc tagtttagca aaacaagccg ggatcacaag
     3781 caacgcagtc aagaaaaccg atcgggattg gttccgtaaa aaccgcgctc accatgaggc
     3841 gatatgcgcg ggcgcggaac ttcatggcag atcattcgct cttcctctat ggcttcttcc
     3901 ttggtttcga ccaacggtcg aagcatcggt taacggttag tagagttcaa ttcatcaccg
     3961 ggagaaatca tgatcgtcaa gagggcgtgg gcagtctgga aaggcggcat caaggacggt
     4021 ggcggcacca tctccacgga aacgggcgtg ctgaaagaag cgccttacgg tttcaaatcg
     4081 cgtttcgaaa acggcaaagg caccaatccg gaggaactca tcggcgcggc gcatgccggg
     4141 tgcttttcca tggcgttgtc gctgatgctc ggcacagcgg gcatgacgcc agagaaaatt
     4201 gagacgcagg ctgaagtctc actcgataag gccggcgagg gcttcgaaat taccgcaagt
     4261 catctaactg tcagggcaaa gattccgggt gccaatccag cgcaatttca ggagatcgcg
     4321 aacaaagcca aggctggctg cccggtatcg aaagtgctga aggcaaaaat caccatggat
     4381 gccagactgg aaacctgact ggttcagact attcgtagcg tccgctcagg ctggcaatga
     4441 gcgccaggag ttcgccgggc tcgaacggtt tcggcagatg cgtctggtag cctgcctgaa
     4501 ttgagcgcac acggtcttcc gcccgtgcaa aggcggtcag cgccacggcc gggatattga
     4561 tgccgcgatg gcgaatcgag cgcatgaagc tatagccgtc cttttttggc attccgatgt
     4621 cactgacgat cacatcgggt ctgaacctct ccatgagctc gagcgcttcg tcggccgacc
     4681 cggcaagcat gggttcagct tcgcaggcgt cgaggaaccg cttgatcatc tcgcgcgcat
     4741 ccggttcgtc atcgacgacg agcaccttca cgccgcgcag cttgcgaggc ggtacgtcgg
     4801 atctggttct cgcagcaatc gccggcacag cgccctgacc cgatcccagg ggcgcgccca
     4861 cgggaagctt taccgtgaaa gtcgcaccct gaccttcgcc actgctggtt gccgcaaccg
     4921 tgccgccgtg catttccgtg ag
//
LOCUS       DATMZQ010000379         3929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2029882, whole genome shotgun sequence.
ACCESSION   DATMZQ010000379 DATMZQ010000000
VERSION     DATMZQ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029882"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VN496_12215"
     CDS             <1..1136
                     /locus_tag="VN496_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HXF17766.1"
                     /translation="SLEGLRDFSVIATAPQRRLAIKTFVSPISDGLIREAVLRELKRG
                     GQVYFLHNDIDSIRRMEERLATLVPEARIRIAHGQMPERELEHVMRDFYQARFNVLLC
                     TTIIETGIDIPTANTIIMNRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPDEDAVGAQ
                     ARKRLEAIQMMEELGSGFYLAMHDLEIRGAGEVLGESQSGEMQEVGFALYAEMLNAAV
                     KSLKAGKEPELSANFNLGTEINLHTPALLPETYCADIHERLVLYKRLANCETLEELEA
                     MQEELVDRFGLPPEPAKCLLETHRLRIGSKALGVTRIDAGPEALILQFVPNPPVDSSK
                     IIRLVQSRKNFKLSGPDRLRIDATMPDVAARTAQVRSIFKELA"
     gene            complement(1176..1499)
                     /locus_tag="VN496_12220"
     CDS             complement(1176..1499)
                     /locus_tag="VN496_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HXF17767.1"
                     /translation="MSDWIFVADAESIAPGHYETVDLDGSIAAVFNIDGQFYAIEDVC
                     THDGTVLTSGTPIEGDEIVCPRHGARFCVKTGEALSPPAYEPTATFPVKVEAGKVYIR
                     DDRWD"
     gene            complement(1569..2075)
                     /locus_tag="VN496_12225"
     CDS             complement(1569..2075)
                     /locus_tag="VN496_12225"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXF17768.1"
                     /translation="MTNGLARSFENADQAQRDELAKKLFAIPHVTNVFYVDNWITVTQ
                     DGEADWQALLREVATPIREAPAAEKTSEEFAKAAAWLAEGNGGADLSESDKLKLEQIN
                     ELLDEEVRPYLQGDGGDLYVVGLEGSTLRVHYQGACGSCPSSISGTLAGIEGLVKRID
                     PAMEVIAV"
     gene            complement(2143..2622)
                     /locus_tag="VN496_12230"
     CDS             complement(2143..2622)
                     /locus_tag="VN496_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HXF17769.1"
                     /translation="MDHKNLYQEVILDHNRKPRNWGRLVDASHRAEGLNPLCGDHIWL
                     SVKVDDGKVGSISFEGESCAICKASSSMMTTNVKGKSIEEAEELIREFRDMATGKLDT
                     GSQPNHLGRLSVFAGVRDLPSRVKCAILPWHTLHAALNSESTVSTEGDRDPVATAER"
     gene            complement(2635..3897)
                     /locus_tag="VN496_12235"
     CDS             complement(2635..3897)
                     /locus_tag="VN496_12235"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXF17770.1"
                     /translation="MSSSSNVLQAARPVPTFDVERIRAEFPILKLKVNGKALAYLDNA
                     ASSQMPQPVIDRFVRYQTAEHANIHRGVHYLSETATAAYEAGRVKIQRFINAREAREV
                     VYTRGTTTSINLVMHGYGRQFIGANDEIIVSQLEHHSNIVPWQMLCEEKGARLKVIQC
                     NDAGELLLDDYARLFNSHTKLVAITHVSNALGTINPIREMVATAHQRGVPVLVDGAQA
                     VPHMKVDVQDLDCDFYAFSGHKMCGPTGIGVLYAKASLLEKMKPFEGGGDMILSVTFE
                     KTTYNVIPHKFEAGTPPIAAGICLGATVDYLEAVGMDRIAAHEQDLLAYANEAFAELK
                     GIRIVGTATHKAGVLSFTVKGVHPHDVGTILNEQGVAVRTGHHCAQPVMQRFGIPATA
                     RASFYLYNTFAEVDALVAGIHKVQKLFS"
BASE COUNT          830 a         1141 c         1071 g          887 t
ORIGIN      
        1 tgtcgctgga gggcctgcgt gatttttcag ttatcgctac tgcgccacaa cgccgtctgg
       61 ccatcaagac tttcgtatcg ccgatcagtg atggcctaat ccgggaggcg gtgttgcggg
      121 agctgaaacg tggcggccaa gtgtacttcc tgcacaacga tatcgattcc atccgccgca
      181 tggaagaacg ccttgcgacg ttggtgccgg aagcccggat ccgtattgcc cacgggcaga
      241 tgccagagcg tgaactggaa cacgtcatgc gcgacttcta tcaggcccgc ttcaacgtgt
      301 tgctatgtac aaccatcatc gaaaccggca tcgacattcc gaccgccaat acgatcatca
      361 tgaatcgcgc agacaaattc ggcctggcgc agctgcatca gcttcgagga cgcgtcggac
      421 ggtcccacca tcaggcttac gcctatttac tgacgccgga cgaagatgca gtcggcgcac
      481 aggcgaggaa gcggctcgag gccatacaaa tgatggagga gctcggctcg ggattctatc
      541 tggcgatgca cgatctggaa attcgcggcg caggagaagt gctgggcgaa tcccaaagcg
      601 gcgaaatgca ggaagtagga ttcgcgctct acgcggaaat gctcaatgcg gccgtcaagt
      661 cgcttaaggc tggcaaggag ccggaactgt cagccaattt caatcttggc accgagatta
      721 atctgcatac gccggcgttg cttcccgaga cctactgtgc cgatattcac gagcgcctgg
      781 tgttgtataa gcgccttgcc aattgcgaaa cgctggaaga gctggaggcc atgcaggaag
      841 aactggtaga ccgcttcggc cttccgccag agccggcaaa atgcttgctg gaaacgcacc
      901 gtcttcgtat cggatccaag gctttgggtg tcacccgcat agatgccggt ccggaagcgc
      961 tcatcctgca atttgtccca aatccaccgg ttgattcatc gaaaatcatc cgcttggtgc
     1021 aatcgcgcaa gaacttcaag ctcagtgggc cagatcgtct gcgcatagat gccaccatgc
     1081 cagacgtagc cgcgcgtacc gctcaggtgc gatcaatatt caaggaactt gcttgaagca
     1141 gtccagtttg gcaccgcagc tatacggcga cggtatcaat cccatctatc gtctctgata
     1201 tagaccttcc ctgcctcaac cttgacggga aacgtggcgg tgggctcgta agctggggga
     1261 gaaagcgctt caccggtttt cacacaaaaa cgcgcgccat gtcgcggaca gacgatctca
     1321 tccccttcga tcggtgtgcc gctggttaac accgtgccgt cgtgagtgca gacatcttcg
     1381 atcgcataaa actggccgtc gatattaaat accgcagcta tacttccgtc cagatcgacg
     1441 gtctcatagt gtccgggcgc gatactttca gcgtccgcga caaaaatcca gtccgacata
     1501 aaacaatgct tgtggctggt gatggatgat tttcagatac gattactgcc aactcaccag
     1561 tgcagcgctc acaccgcaat cacttccatg gccgggtcaa tgcgcttgac caggccttcg
     1621 atgccggcaa gcgtgcccga tatcgaactg ggacatgagc cgcaagcacc ctggtaatgc
     1681 acgcgcaggg tgcttccctc aaggccgact acgtacaggt cgccgccatc gccttgtaga
     1741 tagggccgca cttcttcatc aagcagttcg ttgatctgct caagtttgag cttgtcgctt
     1801 tcggacaggt ccgccccgcc attcccctcc gcaagccagg cggcagcttt ggcgaattcc
     1861 tccgatgtct tttcggcagc cggcgcttcg cggatcggcg ttgcgacctc gcgtagcaat
     1921 gcttgccaat ccgcttcgcc gtcctgagtc accgtaatcc agttgtcgac gtagaacaca
     1981 ttggtcacgt ggggtatcgc aaacagtttt ttggccagct cgtcgcgctg ggcctggtcc
     2041 gcgttctcga acgagcgcgc aagtccattc gtcagggctc cttaagcaca aacttgcgag
     2101 cgttcgggtt cggcgtgtac tcgaggtcgg cgatcttagg catcagcgtt ccgcagtcgc
     2161 gaccggatcc cgatcgcctt cggtcgatac ggttgattca gaattaagtg cggcgtgcag
     2221 ggtatgccac ggcaaaatgg cgcactttac tcgcgacgga aggtctctca caccggcaaa
     2281 tacgctcaag cgtcccaggt gattgggctg gctgccagta tcgagtttgc ctgtggccat
     2341 gtcccgaaat tcgcgaatca gttcctcggc ctcttcgata ctcttgcctt ttacattggt
     2401 ggtcatcatg gacgaagaag ccttgcatat tgcacagctc tctccctcga atgaaatgct
     2461 gcctaccttg ccgtcatcga ctttcaccga cagccagata tgatctccgc aaagcggatt
     2521 caggccttct gcccgatggc ttgcatccac gagcctgccc cagttacggg gctttctgtt
     2581 gtgatcgagg atcacttcct gatagagatt cttgtggtcc attctggcag caagtcagct
     2641 aaataatttc tgcaccttat ggattccagc cacgagcgca tccacttccg caaacgtgtt
     2701 gtaaagataa aaagacgccc gcgcggtggc cggtatgccg aaacgctgca ttaccggctg
     2761 cgcgcagtga tgaccggtgc gaactgccac gccctgttca ttgagaatgg tgcccacatc
     2821 atgcggatga acccctttta cggtaaagga caggacacca gccttgtgcg tcgccgtgcc
     2881 gacgatgcgt atgcctttca gttcggcgaa tgcctcattc gcgtaggcaa gaaggtcctg
     2941 ctcatgggcg gcaatgcgat ccatgcccac ggcctccaga taatcgaccg tcgccccaag
     3001 gcaaatgccc gcagcaatcg gtggcgttcc ggcttcaaat ttatgcggga ttacgttgta
     3061 ggtcgtcttt tcgaatgtaa ccgaaagaat catgtcgccg ccgccttcga acggtttcat
     3121 cttttcaagc aacgatgcct ttgcgtagag caccccaatg ccagtcggcc cgcacatctt
     3181 gtggccggaa aaagcataga agtcgcaatc cagatcctgc acgtccacct tcatatgggg
     3241 aaccgcctgc gcgccatcga ccagcaccgg cacaccgcgt tgatgcgccg tggctaccat
     3301 ttccctgatc ggattgatgg tgcccagcgc attggagacg tgagtaattg ccacgagttt
     3361 ggtatggctg ttgaagagcc tggcgtagtc gtcgagcagt agttcgcccg cgtcgttgca
     3421 ctggatcacc tttaaccgtg cgcctttttc ttcgcacagc atctgccaag gcacgatatt
     3481 ggaatggtgc tctagctggg aaacgatgat ttcatcgttc gccccgatga actgccgtcc
     3541 gtagccgtgc atcaccaggt tgatggacgt cgttgtgccg cgcgtataaa cgacttcccg
     3601 cgcttcccgc gcattgatga agcgctggat tttgacgcgc ccggcttcat aagccgcagt
     3661 cgcggtctca gacaaatagt gcacgccccg atgaatattt gcgtgttccg cggtttgata
     3721 gcgaacgaag cgatcgatca ccggctgtgg catctggcta ctagcggcgt tgtccaggta
     3781 cgccagcgcc ttgccgttga ccttgagctt caatattggg aactccgcac ggatacgctc
     3841 gacatcgaac gtggggactg ggcgcgccgc ctgaagtaca ttgctgctcg aactcatgcg
     3901 gtgcctttca tgtgtgtctg ttcaagaac
//
LOCUS       DATMZQ010000380        11952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1534375, whole genome shotgun sequence.
ACCESSION   DATMZQ010000380 DATMZQ010000000
VERSION     DATMZQ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534375"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VN496_12240"
     CDS             complement(<1..553)
                     /locus_tag="VN496_12240"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXF17771.1"
                     /translation="MSKDLSGTGIVVTRPAAQSDTLARLIRDAGGEPILFPALEVQPL
                     DGVFTEPVLESLASIDLVIFISPNAVRMSMPHILKRGGLTPRTKIAAVGPASAAELRK
                     YGLGKIMLSQKGFDSEALLEELSALPLDRTRVLIVRGQGGRALLGDTLRARGACVDYL
                     ECYRRLKPPNDLLKVCSSLGGTGV"
     gene            complement(550..1485)
                     /gene="hemC"
                     /locus_tag="VN496_12245"
     CDS             complement(550..1485)
                     /gene="hemC"
                     /locus_tag="VN496_12245"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXF17772.1"
                     /translation="MNHPSKIVIATRKSALAMWQARHTAARLKKLYPDVITELLGMTT
                     EGDRKLDTPLAALGGKGLFIKELEVALETGRADIAVHSMKDVPMNLPAGFNIAAISER
                     EDPRDALVSNHYRDLESMPAGSVVGTSSLRRETQLRARCRHLRVEPLRGNVQTRLRLL
                     DEGRYAGIILAAAGLKRLGLSSRITLILEPAYSLPAVGQGALGIECRAERKDMMDLLT
                     PLNHADTRWCVEAERAFSRALAGSCTVPLGGFAEISGTNVRLRGFVGSIDGSCLLFEE
                     LTWPRSGVPEELGSAVAQRLIARGARDILAGAQVS"
     gene            complement(1661..2131)
                     /locus_tag="VN496_12250"
     CDS             complement(1661..2131)
                     /locus_tag="VN496_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HXF17773.1"
                     /translation="MAADLGIEEKDRIKIVEALSRLLADSYTLYLKTHNFHWNVTGPM
                     FQTLHLMFQTQYTELWTNIDLIAERIRSLDAPAPGTYAQFAKLASIQETAGVPKAQDM
                     IRILLEGHEAVTRTARKAFPAAEKGGDQPTMDLLTQRMEVHEKTAWMLRSLLEK"
     gene            complement(2231..3001)
                     /locus_tag="VN496_12255"
     CDS             complement(2231..3001)
                     /locus_tag="VN496_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXF17774.1"
                     /translation="MTHPQALKVVIADDETPARSRVRDLLEDCASSFPIELVGEAASG
                     RELLALLGKASADVVLLDIRMPEMDGLEAAQHLLRLPEPPSIIFTTAYNDHALQAFEL
                     HAVDYLVKPIRLRRLHDALSRARAITPLRLDVLQRIAAEPRKHISVPERGKIMLIDVS
                     DIRYLRAEAKYVTVRTAVREHLVEESLSRLEQEFSEQFVRIHRSCLVAKAHIMGFEKD
                     SIEAGESRWLVLLNGIDERLPVSRRQQHIIKEFGRNLQ"
     gene            complement(2998..4011)
                     /locus_tag="VN496_12260"
     CDS             complement(2998..4011)
                     /locus_tag="VN496_12260"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXF17775.1"
                     /translation="MASINQNSVGTLLPDFRNLGVMLRVLMIVTGLTLVAAILKASNW
                     AAVIQQWIGITAIVQPIAILSVLLLGAANSWLHKLEYRSAVSIIVLAETAITVLVLRF
                     GALVLDIEMDDAGRNALLAAATTIMLIGYFNLRSRALSPALAEARLQALQARIRPHFL
                     FNSINAVLSLIRAEPKRAEAALEDMADLFRVLMADNRKLAPLENEVELCRQYLALEQL
                     RLGPRLRIDWHVDKMPKDALTPPLVLQPLLENAVYHGIEPSIEPGVVSVNIYHVRDQV
                     HMVLRNPYRKDGSHHAGNKMALGNIRERLALHFDAEATLQARVGDSEYEVHITIPYVR
                     AER"
     gene            4084..5490
                     /gene="argH"
                     /locus_tag="VN496_12265"
     CDS             4084..5490
                     /gene="argH"
                     /locus_tag="VN496_12265"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXF17776.1"
                     /translation="MQQQDVGKNNAGQTWSGRFDEPVAELVKRYTASIGFDTRLAEYD
                     IRASLAHARMLQVVGVLSQSDLADIERGMESIMTELGEGKFPWSIDLEDVHLNIEQRL
                     TDLVGDAGKRLHTGRSRNDQVATDIRLYLRAAIDETRALVIALQRRLLDLADLHVDTL
                     MPGFTHLQVAQPVSLAHHLMAYYEMLSRDASRLVDCRKRLNQLPLGAGALAGTTFPIN
                     RALVAKELGFDGICENSLDAVSDRDFAIEFCAAAALIMTHLSRLSEELILWMNPRFGF
                     IRLADRFCTGSSMMPQKKNPDVPELVRGKTGRINGHLVALLTVMKGQPLAYNKDNQED
                     KEPLFDTVDTLRATLSIYADMVDGISVDKQAMRQAAMEGFATATDLADYLVKKGMPFR
                     EAHEAVSRAVRRAVERGVDLAELGIEELKRFSSLIGADVIERLSLEGSLAARDHIGGT
                     APVQVRAAIARARAQIKG"
     gene            5652..6935
                     /locus_tag="VN496_12270"
     CDS             5652..6935
                     /locus_tag="VN496_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF17777.1"
                     /translation="MNNSRKSWMVTLLATVLTAISIGCNAQDKPIRIGLVTFLSGPAA
                     GPFGVPAKLAAEAIVDSLNAGKVPAPYATKGFGGTPLELVIIDEAGGATKQVAELRNL
                     VERQEVDFVIGYIGSGDCLAVAPVAEELKKITVLFDCGTPRIFEDASYKYVFRTRPHA
                     TMDAVAGAMYLLENRPAMKSYGGINQNYAYGQDSWADFTAVMGTLKPDAVITTSQMPK
                     FGAGQYGPEISSLLQSNSDVIHSSLWGGDLEAFMLQATPRNLFSRSQLILVAGEPYLH
                     RLPGSIPDGTIVGARGASGVYAQKSPLNDWLRQIYKERADIYPNYPAYSVAQAFLGLK
                     TAYEKAKSAGATGTDDIIKVFEYETFESPSGKVMMKLGKGHQAVAGTAYGTTRTVNGQ
                     ITVGNVKYYPVERVQPPEGIKSIDWIKAGMKPGKW"
     gene            6952..7848
                     /locus_tag="VN496_12275"
     CDS             6952..7848
                     /locus_tag="VN496_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17778.1"
                     /translation="MGQLVAVLLDGLIYASWLFIIAVGLTLIYGVMKILNIAHGSLYA
                     LGAYSAASLGGYWLKQGHPPMGSYAIMLAAAIVVGLVAGPVIERGLLRFMYGKDEIVL
                     VLVTYAVFLILEDFVKLVWGVDPYFIAQPYGLLGSFDIANLTYPNYNLLLFCAAVFLG
                     VVLAWVLTQTRQGKMLAAVIHDREMSAAMGINVGRVYFITFTVGAILGALGGALTAPM
                     ISVQPGIGAETIVLAFAVVVIGGLGSLPGAALAALLVGLVRSAAVHYRPELDLFSIYL
                     VMAVVLIVRPKGLFSLQEARKI"
     gene            7845..8786
                     /locus_tag="VN496_12280"
     CDS             7845..8786
                     /locus_tag="VN496_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF17779.1"
                     /translation="MKRDPTIAILAAASLILLVIGPILPRWAMFLINVSLCYGIVVLG
                     MMLLMRTGVVSFGHGLYYCLGAYAAGTLDQVYRISDMSVMLLAAILVTALVSAAIGLL
                     LRKYRDIFFAMLSLAFSMILYGLLVKTSALGSSDGFNIAASKIVGITVAGTAGERYVR
                     YALAVVCAFLAGMFMHKYLNSHMGRLALAIRDNELRVEYMGASVRNVIHVNYIISAVL
                     AGIGGALVAIAVGHIDPEMAYWTTSGEFVFVGILSGTGSILAPFLGAFIFETIRSFAY
                     EYSPNTWQMALGITMLVVIMFLPGGLWSLFMRKGKAA"
     gene            8788..9573
                     /locus_tag="VN496_12285"
     CDS             8788..9573
                     /locus_tag="VN496_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF17780.1"
                     /translation="MAIVLEARGLNKSFGAVAAASDINVAVEQDAVVGLIGSNGAGKT
                     TFINMVTGYLKPSSGTITFGGRDITSLSPRQVTRLGICRSFQIPQLFNTLSVYENLLV
                     GVGIVVSGGKEWSRASDHAGTPADAVDGILERFNLMEYRDKLAGVLPEGIRKLLDIAM
                     AMVVKPEILLLDEPTSGVSADEKFSLMDMVMEAVRAQKVTVLFVEHDMEIISRYTQRV
                     LAFYEGRIIADGLPAVVLGDTEVRKYVTGETLHRESGAHHVAH"
     gene            9560..10210
                     /locus_tag="VN496_12290"
     CDS             9560..10210
                     /locus_tag="VN496_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF17781.1"
                     /translation="MLRIEGLDVSIASVGILRNVSMGVPTGKFIGLIGRNGAGKTTLM
                     RSIMGILQPRRGSIQFNGIPVGKNTPVHQRARNGIGYMPEDRRLVPSLTVEENVLIPA
                     WSAGIADPRARLADIYRMIPEVEAFSGRKALQLSGGQQKLVALARALMCGTKLLLLDE
                     PFEGVAPVLARRLAEVIAGLKQQGLSIVLTESDLSHSRELLDVIYSIDRGQVSIVP"
     gene            complement(10503..>11952)
                     /locus_tag="VN496_12295"
     CDS             complement(10503..>11952)
                     /locus_tag="VN496_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HXF17782.1"
                     /translation="ALERELGMPVVETVAVRRGGADALIEAIQIHMSDHPTAAGAWKA
                     PDIEDVASTQREVRRILAATVQEPLDELKMDDALDRIVLHPFWGYVILALTLFMMFQA
                     VFSWAQLPMDMIKSAVQAIGAMLKAWLPAGTLQSLLADGVVAGAGSVLVFLPQILILF
                     LFILALEDSGYLPRAAFMLDRLMGTVGLSGRSFIPLLSSFACAVPGIMSTRNIANWRD
                     RLTTIMIAPLTTCSARLPVYALIIGAFIPQRTILGFLNLQGVVLFVLYLAGIVSAMAV
                     AWTMKVAAGRKMLHPLMMELPSYRWPHLKNLFIGLVERAKIFLTRVGTIILSLTILLW
                     FLSSFPAPPVDASGPAIQYSLAGMLGRALETVLAPIGFNWQISIALIPGLAAREVAVG
                     ALGTVYALSATGDDVAAQLTPMIAQSWPLATAFSLLAWYVFAPQCLSTLSIVRRETNS
                     IRYPLIMAGYLFALAYLASFITYRIALVLVGS"
BASE COUNT         2631 a         3335 c         3365 g         2621 t
ORIGIN      
        1 tgacgccagt cccacccaag ctcgaacaga ctttcagcag gtcgttaggc ggcttcaatc
       61 gacgatagca ttccagataa tccacgcaag cgccgcgagc gcgcaaagtg tcaccgagca
      121 aggctcgccc accctgcccc cggacgatca acacacgcgt tcggtccagc ggcaaagcag
      181 agagttcctc aagcaaggcc tcgctatcga agcctttctg cgacaacatg atctttccca
      241 gtccatactt tctcaactct gcggcgcttg cgggtccaac cgcggcaatt ttggttcgcg
      301 gagtcaatcc gccgcgcttc agaatatgcg gcatcgacat gcgtacggca ttcgggctga
      361 tgaaaatcac cagatcgata gacgccaggg actcgaggac aggttcagta aatactccat
      421 ccagcggctg gacttccagc gccggaaaca aaatcggctc tccgccagca tcgcgaatca
      481 ggcgcgcgag tgtgtcggat tgcgccgctg gccgggtgac gacaatcccg gtgcccgaca
      541 gatccttgct catgaaacct gcgctccagc cagtatgtcg cgcgcgcctc ttgcaatgag
      601 ccgctgcgca acggcacttc caagttcctc tggtacgccc gatctgggcc aagttagctc
      661 ttcaaaaagc aggcagctgc catctatact gcccacgaaa ccacgcaggc gaacgttcgt
      721 gccgctgatc tctgcgaagc cgcccaaggg caccgtacaa ctgccggcca gcgctctgct
      781 aaacgcacgt tccgcctcta cgcaccatcg cgtatcggca tgattcagcg gggttagcaa
      841 gtccatcatg tccttgcgtt cggcacggca ctcgataccg agcgcgccct ggccgactgc
      901 tggaaggctg taggccggtt caagaatcaa cgttattcgc gaggacagac cgaggcgttt
      961 gagcccagca gctgccagaa taatgccagc gtaccggccc tcatccagca agcgcaaccg
     1021 agtctgtaca ttgccgcgca aaggttccac gcgcaaatgc cggcaacgtg cccgcagttg
     1081 cgtttcgcgc ctgagacttg atgttccaac aacgcttccc gctggcatgg attccagatc
     1141 tcgatagtga ttcgatacta gagcatcgcg tggatcttcc cgctcgctga tcgcagcaat
     1201 gttaaagccc gccggcaaat tcatcggcac atctttcatt gaatgaaccg cgatgtcggc
     1261 ccgccccgtc tcgagcgcaa cctccagctc cttgatgaac aatcctttgc cgcctagcgc
     1321 cgcaagcggc gtgtcgagct tgcgatcgcc ttccgtggtc atcccgagca actcagtaat
     1381 gacgtccgga tacagttttt tcagcctggc tgccgtatga cgggcctgcc acatcgccaa
     1441 ggcactcttg cgtgtggcaa tcacgatttt cgatggatgg ttcaagacgg aagaaagagt
     1501 tttactgcct tagaaaacgt tacgactcag ggttcagctg cagccccgcc tggcaccgtc
     1561 aatcgtgatt aacggtcacg cttgacggtc gcgtatcgat ccatatttat tgcgccgccg
     1621 ttgcaccgct gattgcgtcg gcggcttcac ttgagacttg ttatttctcc aacaagctcc
     1681 tcaacatcca ggcggttttt tcatgtactt ccatacgctg agtcaacaaa tccatggttg
     1741 gctgatcgcc tcctttttct gctgcgggaa acgctttgcg tgcggtacgg gtaacggcct
     1801 cgtggccttc cagcaaaatc cgaatcatgt cctgcgcctt ggggacaccc gccgtttcct
     1861 ggatggatgc cagcttcgcg aactgtgcat aggtgccggg cgcgggcgca tcgagtgatc
     1921 gaatgcgttc tgcgatcaga tcgatgttag tccacagctc cgtgtattgc gtctggaaca
     1981 tcaggtgcag cgtctggaac atcgggccgg tcacattcca gtgaaaatta tgcgtcttca
     2041 aatacagcgt gtagctgtcc gccaacagcc gggacagagc ctcgacaatc tttatgcggt
     2101 ccttctcttc gataccaaga tcagcagcca tgtactttcc ttgtttagtg ttatgtcgat
     2161 ggaaccgttc gcagaatcat aggccaattc cgcgcagcct tcaggcggac cgccttctca
     2221 atgcactgcc ctattgcagg tttcgtccaa attccttgat gatgtgctgt tgtcgccggc
     2281 tcaccggtag tctttcgtct attccattga gcagcaccaa ccagcgtgat tcgccggctt
     2341 cgatggaatc cttctcgaag cccatgatat gggccttggc caccagacaa ctacgatgaa
     2401 tacggacgaa ctgttcgctg aactcctgtt cgaggcgaga gagcgactct tccaccagat
     2461 gttcgcgcac agcagtgcgc acagtcacat atttggcctc ggcccttaaa tatcggatat
     2521 cggacacgtc tatcagcatg atcttgcctc gttcaggcac tgagatatgt ttgcgcggct
     2581 cggcggcgat gcgctgaagg acgtcgaggc gtaacggcgt gatggcccgc gctctggata
     2641 acgcatcgtg caagcgccgt aatcgtatgg gcttgaccag gtaatcgacc gcgtgaagtt
     2701 caaaagcctg caaggcatga tcgttatagg cagtcgtaaa aattatcgat ggaggttccg
     2761 gaagtcttag caaatgctgc gcggcttcaa gtccatccat ttctggcatg cgaatgtcga
     2821 gcaacacgac atccgcggac gctttgccga gcaacgccag caactcgcgt ccgctcgcag
     2881 cttcgccgac gagttcgatg ggaaagctgc tggcgcaatc ctcgagaaga tcccgcacgc
     2941 ggcttcgggc gggcgtttca tcatccgcta tgacgacttt caaagcctga ggatgtgtca
     3001 ccgctctgcc ctgacgtacg gaattgtaat atgcacttca tactcgctgt ctccaactcg
     3061 cgcctgcaag gttgcctctg catcaaagtg cagggcaagc ctctctctga tattgccaag
     3121 ggccatcttg tttccggcat ggtggctgcc gtctttgcga taggggttgc gcaagaccat
     3181 atgtacttgg tcgcgcacat ggtaaatatt gacactgact acacccggtt cgatggatgg
     3241 ttctatgccg tggtagacgg cattttccag gagcggctgc aacacaagag ggggcgtaag
     3301 tgcatccttc ggcatcttgt ccacatgcca gtcaatgcga aggcgcggac ccagacgcaa
     3361 ttgttcaagt gcaagatact gacgacacaa ttcgacttcg ttctccaatg gcgccagctt
     3421 tcgattgtct gccatcagaa cgcggaaaag atcagccata tcttccagtg ccgcctccgc
     3481 gcgtttcggt tcagcgcgta tcaacgacaa cactgcgttg atactattga atagaaagtg
     3541 ggggcgaata cgagcctgca gcgcctgaag cctggcttcc gccaatgccg gggacagtgc
     3601 gcgactacgc agattgaaat agcctatcag catgatcgta gttgcagccg cgagaagcgc
     3661 attacgtcct gcatcgtcca tttcaatgtc cagaactagt gccccgaagc gcagcaccag
     3721 cacggtaatg gcggtctcgg ccaatacgat tattgagact gccgatcgat actccagctt
     3781 atgcagccag gaatttgccg cgccaagcag caacactgaa agaattgcga tcggctgcac
     3841 gattgctgta ataccgatcc attgctgtat cacggcggcc caattggacg cttttaatat
     3901 cgccgcaaca agagtcaacc ccgtcacaat catcagcaca cgcaacatga cgccgagatt
     3961 gcgaaaatca ggcaacaaag taccgaccga gttttgattt atacttgcca tctaaaaagc
     4021 gtgctgaaac gctcggcaaa acaattgttt aacgcattcc attgtgaacg gggcagaccg
     4081 tcaatgcaac agcaggacgt gggtaaaaac aacgctggcc agacatggtc tggccggttc
     4141 gatgaaccgg ttgcggaact ggtcaagcgc tatacagcat cgatcggctt tgatacaagg
     4201 ctcgcggaat atgacattcg ggcgtctctt gcccatgcgc gcatgctgca ggtggtaggc
     4261 gtactgtccc aatcggatct cgccgatatc gagcgcggta tggaaagcat aatgactgag
     4321 ctaggcgaag ggaagtttcc ctggtcgatt gatcttgaag atgttcacct caacatcgaa
     4381 caacgtttga ccgatctggt cggggatgca ggtaagcgat tgcataccgg ccgctcgcgg
     4441 aacgaccagg tagcaaccga catccggctt tacttgcgcg ccgcaattga tgaaacgcgt
     4501 gcgctggtta tcgcgttgca aaggcgactg ctcgatctgg ccgatctgca tgtggatacg
     4561 ctgatgcctg gttttacgca tctgcaggtc gcacaaccgg tgagtcttgc gcatcacctc
     4621 atggcctatt atgaaatgct gagccgtgac gcatcacggc tggtggattg cagaaagcgg
     4681 ctcaatcaac tcccgctagg tgccggcgca ctcgctggta ccacattccc gatcaaccgc
     4741 gcgcttgtgg ccaaagaatt gggcttcgat ggtatctgcg aaaactctct cgatgcggta
     4801 tcggaccggg acttcgccat cgaattttgc gctgccgccg ctctgatcat gacgcacttg
     4861 tcccggcttt ccgaggaatt gatcttgtgg atgaacccgc ggttcggctt tatccgtttg
     4921 gctgatcgat tctgcacggg ttcttccatg atgccgcaga aaaagaatcc ggatgtgcca
     4981 gagctcgtgc gtggcaagac aggccggatc aacggacacc tggtcgcttt gcttaccgtc
     5041 atgaagggac agccgctggc ctacaacaaa gacaatcagg aagataagga accgttattc
     5101 gataccgtag atacgctgcg cgcgacgttg agcatctatg cggacatggt agacggtatc
     5161 agcgtggata agcaggcgat gcgccaagcc gcaatggaag gctttgcgac ggccaccgac
     5221 ctggccgact atctggttaa aaaaggaatg ccttttcgcg aagcgcatga agcagtctcg
     5281 cgggccgtgc gtcgtgccgt tgagcgaggc gtcgatcttg cagaattggg tattgaagaa
     5341 ttgaagcgtt tctcgagctt gatcggggcc gatgtcattg aaaggctatc cctggaaggc
     5401 tcgctggcgg cacgcgacca tatcggcggt acggcgcccg tccaggtacg tgctgccatt
     5461 gctcgggccc gcgcgcaaat caaggggtag ccgccagtta tgcggccttc atcgttgggc
     5521 ggtcctacga cctatgacga attgctgtgt aaggtcaaaa gctgctagat tccgactgga
     5581 tttgtatcgc ttgggaaggc agcgtcactc gctcgagtgc tcgctgaaaa ctaaaaattc
     5641 ttggggggcg catgaacaat agtcggaagt cctggatggt aacgctgctt gcgacagtgc
     5701 tgactgccat atcaattggc tgcaatgcac aggacaagcc aatacgcata ggtttggtga
     5761 ctttcctgtc gggtccggca gcgggtccgt ttggtgtacc cgcgaaactc gctgctgaag
     5821 caattgtgga ttcactaaat gcgggaaagg ttccagcacc ttatgccacc aagggcttcg
     5881 gcggtacccc gcttgagcta gtcataatcg atgaggcagg tggcgccacc aagcaggtag
     5941 ctgaactacg caatctggtt gagcggcaag aagtcgattt tgtgatcggt tacatcggca
     6001 gtggcgattg tttggcggtg gctccggtag ccgaagaact gaagaaaatc acggttctgt
     6061 tcgattgcgg aacgccgcgt atatttgaag atgccagcta caagtacgtg ttccggaccc
     6121 gcccgcacgc caccatggat gccgtagccg gcgcaatgta cctgctggaa aaccggccgg
     6181 ccatgaaaag ctatggcggc atcaatcaga attacgccta cggtcaagat tcgtgggcgg
     6241 acttcactgc ggtcatgggc acgctgaaac ccgacgcggt aatcacgaca tcccagatgc
     6301 caaagttcgg agccggacaa tatggtcccg agatttcctc gttgttacaa agtaactccg
     6361 atgtcattca ttcgagcctg tgggggggag acctggaagc cttcatgttg caggccacgc
     6421 cacgcaattt attctcgcgt agccagctca ttctggtggc cggtgaacct tatttgcata
     6481 gattgcccgg cagcattcct gacggcacca ttgtcggggc gcggggcgcc agtggcgttt
     6541 atgcgcaaaa aagccccctg aacgactggt tgcgccaaat ctataaagag cgcgcagaca
     6601 tatacccaaa ctatccggcc tacagcgtcg cgcaagcatt tctgggactg aagactgctt
     6661 atgaaaaagc gaaatcagca ggcgcaaccg gtactgatga catcatcaag gtctttgaat
     6721 acgagacgtt cgaatcgcca agcggcaagg tgatgatgaa gctcgggaag ggccatcaag
     6781 cggtagcggg tacggcgtat ggcacaacgc gcacggtaaa tggccagatc acggtaggga
     6841 atgttaaata ttatcccgtg gaacgtgtgc agccgcccga ggggatcaaa agcatcgatt
     6901 ggatcaaggc aggaatgaag cccggcaagt ggtaaggcct cctagcggac catgggacaa
     6961 ctcgttgccg tcctgcttga tgggttgatc tacgcgtcct ggcttttcat catcgccgtc
     7021 ggcctgacgc tgatctacgg cgtgatgaaa atactcaata ttgcgcacgg cagcctttat
     7081 gcgctcggtg cctacagtgc ggcatcgctc ggcggctact ggctcaagca gggtcatccg
     7141 ccaatgggga gctacgccat catgctggcg gcggccatcg tggtgggtct ggtcgccggc
     7201 ccggtaatcg agcggggatt gctgcgtttc atgtacggca aagacgaaat cgtgctggtt
     7261 ctggtcacct atgcggtatt tctgattctg gaagatttcg tcaagctggt atggggagtc
     7321 gatccctatt tcatcgcgca accctacggg ctattgggta gcttcgatat cgcaaatctg
     7381 acttacccga attacaacct gctgctgttc tgtgccgccg tcttccttgg tgtggtactc
     7441 gcttgggtac tgacccagac tcgccagggc aaaatgctgg ccgctgtaat tcatgatcgt
     7501 gagatgtccg cagccatggg catcaacgtc ggtcgggttt acttcattac cttcacggtc
     7561 ggcgccatac tcggcgcgct cggcggtgca ctgactgcac cgatgatttc ggtgcaaccc
     7621 ggtatcggcg ccgaaacaat cgtcttggca ttcgcggtgg tcgtgattgg cgggctggga
     7681 agtctgcccg gcgcggcgct tgcagctttg cttgtcgggc tcgtacgctc tgccgccgtg
     7741 cactaccgac ccgagctgga cttattcagc atttatctgg tcatggcggt cgtactgatc
     7801 gtccgcccca aaggcctgtt cagcttgcag gaagcgcgca agatatgaag cgagatccga
     7861 ctattgcaat actcgccgca gcatcactga tcctgctggt tatcgggccc atcctgccgc
     7921 gctgggcgat gtttctcatc aatgtgtcgt tgtgctatgg cattgtcgta ctcggcatga
     7981 tgctgctcat gcgcacgggt gtggtgtcgt ttggacacgg tctgtactac tgcctgggcg
     8041 cctatgccgc gggcacgctt gaccaggtgt acaggatctc ggatatgtcc gtaatgctgc
     8101 tcgccgcaat cttggtcacc gctttggtgt ccgccgcgat tggactgctg cttcgcaagt
     8161 acagggacat tttcttcgcg atgctgtcgc tcgccttttc catgattctt tacggtttgc
     8221 tggtcaagac ctccgctctt ggatcgagtg acggtttcaa catcgcggcg tcaaaaatcg
     8281 tgggcatcac ggttgcggga acagctggag aacgatacgt tcgctatgcc ctggcagtgg
     8341 tatgtgcctt ccttgcgggc atgttcatgc acaagtacct caattcccac atgggacggt
     8401 tggctctggc gatcagggac aacgagttgc gcgtggaata catgggcgcg tccgttcgca
     8461 atgtcatcca cgtgaactat ataatttccg ccgtgctggc cggtatcggt ggtgcgcttg
     8521 tggcaatcgc cgtgggccat attgatcccg aaatggccta ctggaccacg tcgggcgagt
     8581 tcgtcttcgt gggcattttg tccgggacgg gtagcatatt ggcgccgttt ttaggtgcct
     8641 ttatctttga gaccatccgc agcttcgcgt atgaatattc gccgaacacc tggcagatgg
     8701 cgctgggtat tacgatgctt gtcgtaatta tgttcctgcc tgggggccta tggtccctgt
     8761 tcatgcgcaa gggcaaggca gcctgacatg gcaatcgtcc tggaagcgcg cggtctcaac
     8821 aaaagttttg gggcagtggc cgccgccagc gatatcaacg ttgcagtcga gcaggatgcc
     8881 gtggtcggat tgatcggcag caatggggcc ggaaagacca cattcatcaa catggtgact
     8941 ggttatctca agcccagctc cggcaccatt accttcggtg gccgcgacat cacatcgttg
     9001 agtccccgtc aggttacgcg gctgggcatc tgccggtcat ttcagatccc gcaactgttc
     9061 aatacgctgt ccgtctacga gaatcttttg gttggcgtcg ggattgttgt atccggagga
     9121 aaggagtggt cgcgggccag cgaccatgcg ggcacgccgg cagatgccgt agacggcata
     9181 ctggagcgct tcaacttgat ggaatatcgg gacaaactgg ccggcgttct gccggaggga
     9241 attcgcaaac tgctcgatat cgcgatggct atggtagtca agcccgagat cctgctgctc
     9301 gacgaaccaa catccggcgt gtcagcagac gagaagttca gccttatgga tatggtaatg
     9361 gaagcagtgc gcgcacaaaa agttactgtg ctgttcgtcg aacacgacat ggaaataatt
     9421 agccgctata cgcagcgcgt actcgctttc tacgaagggc gcatcattgc cgacgggctg
     9481 ccggccgtgg tgctgggcga cacggaagtg cgcaagtatg tcaccggcga aacgcttcac
     9541 cgcgagtccg gagcgcatca tgttgcgcat tgaaggtctc gacgtatcca ttgcatcagt
     9601 cggcattctg cgcaatgtga gcatgggagt tcccaccgga aaattcattg gactcatcgg
     9661 tcgcaacggc gccggcaaga ccactttgat gcggtccata atgggtatat tgcagccccg
     9721 ccgcggaagc atccagttca acggtatacc ggttggaaaa aacacgcccg tgcaccaacg
     9781 tgctcgcaat gggattggct acatgccgga agaccgtcgt ctggttccct ctcttacggt
     9841 cgaggaaaac gtgctgattc ccgcttggtc cgctggcatt gccgacccgc gtgcgcggct
     9901 agccgatatt taccggatga tccccgaggt ggaggcgttc tccgggcgca aggcattgca
     9961 actttccggt gggcagcaaa aactagtggc gctggcacgg gcactgatgt gcggcaccaa
    10021 actgttgtta ttggacgaac cgttcgaagg ggtggcaccc gtgctggcga ggcggctcgc
    10081 ggaagtcatt gcgggcctga agcagcaggg tttgtcgatt gtgttaacag agtcggatct
    10141 ctcgcactct cgcgagttgt tggacgtgat ctattcgatc gatcgcggcc aagtctctat
    10201 cgtaccttga gtaggcgccg cgcagtgcta gccgcatcca aacgcatgaa actagcgtcg
    10261 atgtagccgt attggcttcg ctattgaatt gggtttcggg tcagtttcgc atcccttgca
    10321 actgctacat cccacatcgc ctgcatcgtt ctgcagacgc tcgagtcgag ctcgctgaga
    10381 accgggcaca acgttaagca ggcaggcgat cagccagcgg cgggcagccg ccggcatgaa
    10441 cagccacgcg gcatacaaga gtgcggtagt gacaattgcg tacgtaatga aattttgcat
    10501 gatcaactgc ccaccagtac aagcgcgatc cggtacgtaa taaaagaagc gaggtaagcg
    10561 agcgcaaaaa ggtaaccggc catgatcagt ggatagcgta tcgaattggt ttcacgccgc
    10621 acaatggaca gcgtagagag gcactggggc gcaaagacgt accatgccag cagtgaaaaa
    10681 gccgttgcca gtggccaact ctgcgcaatc attggcgtta gctgcgccgc gacgtcatcc
    10741 ccggtcgccg acaaggcata taccgttcct aaggcgccga ctgctacctc acgtgcggca
    10801 agaccaggta tgagcgcgat cgaaatctgc cagttaaaac caatcggcgc cagaacggtt
    10861 tccaatgctc gcccaagcat gccggcaagg ctgtactgga tcgccggtcc ggacgcatcc
    10921 actggcggag ccgggaacga tgacaagaac cacagaagga tagtcagcga aaggataatg
    10981 gtgccgaccc gggttagaaa aatctttgcc cgctccacga ggccgatgaa caggttcttt
    11041 agatgcggcc accgatacga gggcaattcc atcatcaagg ggtgcagcat tttgcgtccg
    11101 gccgcaacct tcatggtcca ggctaccgcc atcgcgctca caatgcccgc cagatacaga
    11161 acaaacagga ctacgccctg gaggttcaag aagcctaaaa tggtcctttg tggaataaaa
    11221 gcaccgatga taagtgcgta aaccggcagc cgcgccgaac atgttgtgag gggcgcaatc
    11281 ataatggtgg tcagtcgatc gcgccaattc gcaatgttgc gcgtagacat gatgccggga
    11341 accgcgcacg cgaagctcga tagaagcggt ataaaggagc ggccagaaag cccaactgtc
    11401 cccatcagcc gatcaagcat gaatgcggcc cgcggcaaat aacccgaatc ctcgagagcc
    11461 aggataaaca gaaacagaat cagaatttgc ggcaggaata ccagaacgct gcccgcaccc
    11521 gccactacac cgtccgcaag caggctttgt agcgttccag cgggcaacca tgcttttagc
    11581 attgcgccga tggcctgaac tgccgacttg atcatatcca tcggcaactg cgcccagctg
    11641 aacaccgcct ggaacatcat gaacaacgtc aaagccagaa tcacgtagcc ccagaacgga
    11701 tgcaggacaa tccggtcgag cgcatcatcc atttttaatt cgtccagcgg ttcctgtacg
    11761 gtggccgcta gaatgcgccg gacctcgcgc tgagtactcg ccacgtcttc gatatccggg
    11821 gccttccatg cacctgcggc cgtcggatgg tctgacatat gaatctggat ggcctcgatc
    11881 agagcgtctg caccgccgcg ccgcaccgcc acggtttcca cgaccggcat gccaagctct
    11941 cgctccagtg ca
//
LOCUS       DATMZQ010000381         6845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3409215, whole genome shotgun sequence.
ACCESSION   DATMZQ010000381 DATMZQ010000000
VERSION     DATMZQ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3409215"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VN496_12300"
     CDS             complement(<1..743)
                     /locus_tag="VN496_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXF17783.1"
                     /translation="MALETYRQKRNFSITPEPKGRKPKVKKNALAFVIQKHAASRLHY
                     DFRLELNGVLLSWAVPKGPSLDPNDKRLAMHVEDHPIEYGEFEGIIPPKQYGGGTVLL
                     WDKGTWLPKEDPAAGYAKGKLKFDLHGEKLQGGWTLVRSRGSKYAGDKAWLLIKENDE
                     FARRGADALIVEDRPKSVATGRSIEEIAAERDRVWESNKSVQENVKSGAVAKKKKRKI
                     DASSVEGAVPRQIPDFIEPQLATLVKTAP"
     gene            complement(753..1574)
                     /locus_tag="VN496_12305"
     CDS             complement(753..1574)
                     /locus_tag="VN496_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF17784.1"
                     /translation="MRSTYTVKEAQKILGIPLSAIRKFVGNGFVSPTRGSRREYRFSF
                     HDLVVLRMAKALFDASLSPRRIGVCLKRLRKQLPDTLPLAGLRISAMGKDVIVKERDA
                     RWRAEDGQYLLALDVSESHGAVSFSQPPPSRDQTDWFARAFELEALDPAQSMVAYQKA
                     IEQDPSFAPAYANLGRLLHAAGRGNEAETVYLEGETACPDDPIVLFNFALLKEDQERN
                     EEAIGLYKRALAADPAMGDAHYNLGLLYQSLRRQRDALRHFSAFRKLSAGSNSTE"
     gene            complement(1587..2453)
                     /locus_tag="VN496_12310"
     CDS             complement(1587..2453)
                     /locus_tag="VN496_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXF17785.1"
                     /translation="MAARSIASLTVSFGLVSIPVKLYSATQARASISFNLLHAKDGSR
                     LRQQYVCIKEDVVVERDEMIKGYEFAKDQYVTFTADELKALEEESTGTVEITEFVPDE
                     KIDPIYYDKAYYLAPDKGGAKPYALLSEGMRSTGRCALARWAARGKQYLVQLRPVADG
                     LMMQQLLYSDEVRPMSEIEIQHSPVKEAELKLAVQLIDQISADEFNPKAYEDDVKKRI
                     EAAVEKKISGKEISVSPAAPTGKGAQVIDLMEALRASLNKGTPKPEQAERKPPKRAAA
                     AARPAKAKAARR"
     gene            complement(2565..4025)
                     /locus_tag="VN496_12315"
     CDS             complement(2565..4025)
                     /locus_tag="VN496_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXF17786.1"
                     /translation="MITLAVMSATLIQVLDTTIVNVALPHMAGELGATTDQISWVLTS
                     YLVSSAIVMPLTGYFSDTRGRKRFLLVCIAGFVAASALCGISQSLAEIVGFRLLQGVF
                     GAALVPLSQAIMTEAYPVEERGKAMAIWGLGVMVGPVLGPTLGGWLTEYASWRWTFYI
                     NVPIGALSFFLASQYVHNAPRKSRRMDWAGLALLGSGVAGLQYMLDRGTTQDWFAAID
                     IRVAAALAIVGISGFIWHSLRRGENALFDLHIFRDRNFAMACLVMAALGLGLFGGLVL
                     QPILLEGLLDYPIVTTGFVMAPRGLATAMSMIVVGRLVNRADARLLVGSGMLISAAGS
                     YAMTWYSLDVSTMWVIWPAFLQGMGLGLIFVPLSTIAYATLDRARMAEAAGIYSLVRT
                     IGAAAGISVVTTLMTRQAQVIWIQLGGHVTQYNSALLEYLRPLHLSPTDPHAIALVAA
                     ELARQAQMGAMLAVFKVTTWSFLGMLPLVFLLRRSR"
     gene            complement(4043..4480)
                     /locus_tag="VN496_12320"
     CDS             complement(4043..4480)
                     /locus_tag="VN496_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXF17787.1"
                     /translation="MFHRVLLCYDGTREGRNALRQGADVVICMRSEVYLLAISRNLVS
                     TAIPEGVTPELCAQEEERADALLQEGIAWLRERGVAAEGSLEYGNAADCIAATAQRID
                     ADLIVLGHKPRGRLARWWSESEEQSLLERVSCSILVATASGAE"
     gene            complement(4484..5542)
                     /locus_tag="VN496_12325"
     CDS             complement(4484..5542)
                     /locus_tag="VN496_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXF17788.1"
                     /translation="MASSAIDDSATPAHSAPVLVPHRLGRKQLKWIIAGIVLVVLAIA
                     AIAYWREISRYVSTDDGYVQANQVEVTAQISGPVIKVYVRDQQAVKVGDPLFDIDPAN
                     YELALQKAQAQLELARQSVSQESASVASAQALLAQRRAEAANARRNWNRNQKLMRSGF
                     VSPQGAENSRTELATAEAAVKAAEANVAQAKSALGRLGDQNANVQSAITAVRQAQLDL
                     ERTRLTSPVNGVVANLTLQPGNTVQPGVPVFVVISDEEYWIDANFKETELVDIHPGQK
                     AIISSDVYPDRVFHGVVESLSGGSGTAFSLLPPQNATGNWVKVTQRVPVRIKVEDPDP
                     QHPLRIGTTATVKVVKSG"
     gene            5854..6504
                     /locus_tag="VN496_12330"
     CDS             5854..6504
                     /locus_tag="VN496_12330"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HXF17789.1"
                     /translation="MRLSVGILFALLLSVMDCMGETWLRQSVCWPGTAVERPSAMEDH
                     EEKLSLRQAAIFLLEECRTILPGIQALFGFQLIAIFNDGFDTKLSSSEQDLHLAAISL
                     VAVAVVLIMAPAAYHRQTGPMKVNARFITVASRAALLAMIALMLGLTLDFYVVAQVVL
                     GERKWSLLLAALLCGLIVTVWFMLPRTRALRRYASAGDHLHEPAKDQRGASTHHRD"
BASE COUNT         1381 a         2041 c         1914 g         1509 t
ORIGIN      
        1 tccggcgctg ttttaaccag cgtcgcgagc tggggctcga tgaaatccgg aatctgacgc
       61 ggcaccgcgc cttcaacgct ggaagcatca atcttgcgtt tctttttttt tgctaccgct
      121 ccgcttttga cgttttcctg aaccgacttg ttggactccc atacgcggtc gcgctctgcc
      181 gcaatctctt cgatgctgcg tccggtagcg acgctcttgg gtctgtcctc cacaatgagt
      241 gcgtccgccc cgcgcctggc aaattcgtcg ttctccttga tcagcagcca ggccttgtcg
      301 ccggcatact tgctgccgcg gctccgaacc agcgtccagc cgccttgcaa cttttccccg
      361 tggagatcga atttgagttt tcctttcgca taaccggcag cgggatcttc cttcggcagc
      421 caggttccct tgtcccacag caataccgtt ccgccaccat actgcttggg cgggatgatg
      481 ccttcgaatt ccccgtattc gattggatga tcttcgacat gcattgcgag ccgcttgtca
      541 ttgggatcga ggctcggtcc tttgggtact gcccagctca gcagcacacc gttcaactcc
      601 aaccgaaaat catagtgaag acggctggcg gcgtgcttct gtataacaaa agcgagcgcg
      661 ttttttttaa ccttcggctt ccgccctttt ggctccggcg tgatactgaa gttgcgtttc
      721 tgacgatagg tttccagcgc cacgacttaa ccttactccg tggagtttga acccgcggaa
      781 agtttgcgaa aggcactgaa gtgccgaagc gcatcgcgct ggcgccgcag ggattggtag
      841 agcagaccga ggttgtagtg ggcatccccc atggcggggt cggcggcgag cgcacgtttg
      901 taaagcccga tggcttcctc gttacgctcc tggtcttcct tcagcagtgc gaaattgaac
      961 aacacgatcg ggtcgtcggg gcaagcggtc tcgccctcga ggtacacggt ttctgcctcg
     1021 ttgccgcgtc ccgctgcgtg cagcagccgg ccgagattgg catacgcggg cgcaaaggat
     1081 ggatcctgtt cgatagcttt ctgataggcg accatcgatt gcgctggatc gagagcttct
     1141 agctcaaacg cacgcgcgaa ccaatcagtc tggtctcggg aaggaggcgg ctgggagaac
     1201 gatacggcac catggctttc cgatacgtcg agcgccagca ggtattggcc gtcctcggcg
     1261 cgccagcggg catcgcgttc tttcacgatc acatccttgc ccatcgcgct gatgcgaagc
     1321 cccgccaaag gtagcgtgtc cggcaattgt ttgcgcagcc gcttcaggca aacaccgata
     1381 cggcgtggcg acaagctcgc atcgaacagt gctttcgcca tgcgcagcac caccagatcg
     1441 tgaaaactga accgatattc ccgtcgggag ccccgcgtcg gcgagacaaa cccatttccc
     1501 acaaacttgc gaatggccga cagcgggatg ccaagtattt tttgcgcttc ctttaccgtg
     1561 taggtcgatc gcatccccga tgccagttag cgacgcgccg cctttgcttt ggcgggccgg
     1621 gctgccgcgg ccgcccgttt cggaggttta cgctcggctt gctcgggttt tggcgtccct
     1681 ttgttcaggc tggcgcgcaa tgcttccatc agatcgatga cctgagcacc cttgccggtg
     1741 ggagcggccg gcgacactga aatttccttg cccgatatct ttttctcgac ggcggcttca
     1801 atgcgcttct tgacatcgtc ttcgtacgcc ttgggattga attcatccgc ggagatctga
     1861 tcgatcagct gcaccgcaag cttgagttct gcctctttca cgggcgaatg ctggatttcg
     1921 atttcggaca tgggccggac ttcatccgaa taaagcagtt gctgcatcat gagaccatcg
     1981 gccaccggcc gcagctgcac gagatattgt ttcccgcgtg cggcccagcg tgcgagcgcg
     2041 caacggcctg tgctgcgcat gccttcgctc agcaatgcat atggttttgc cccgcccttg
     2101 tcgggagcca ggtaatacgc cttgtcgtaa tagatgggat cgattttttc atccggtacg
     2161 aattcggtta tttcgaccgt gcctgtggat tcctcttcga gagccttcag ctcgtccgcg
     2221 gtgaaagtca cgtactggtc cttggcaaac tcatagccct tgatcatctc gtcgcgctcg
     2281 acaaccacgt cttccttgat gcaaacgtat tgctggcgca agcgcgagcc gtccttggcg
     2341 tgcagcagat tgaaggagat gctcgcccgc gcctgcgtgg ccgagtagag cttgaccggt
     2401 atcgaaacca ggccaaacga aaccgttaaa gacgcaatgg atcgggccgc catgatttcc
     2461 tcacattgat tagtacttca taagcttagc actggttctt aaacttgaca cgcatatgct
     2521 gacggttaga catcattctg ttgcgaattg ctcaccttgt ccgtttaccg gctgcggcgc
     2581 aaaaggaaca cgagaggcag catgccgaga aagctccatg ttgtaacctt aaagaccgcc
     2641 agcatcgcgc ccatttgtgc ctgcctggcg agttccgccg cgaccagggc gatggcgtgc
     2701 ggatcggttg gcgacagatg cagcggacgc agatactcca ggagcgccga attgtattgg
     2761 gttacatggc cgcccagctg gatccagatg acctgcgcct gccgtgtcat caaagtggtc
     2821 acgaccgaaa ttcccgcagc tgcgccgatg gtacgcacaa gactataaat gcctgctgct
     2881 tctgccatgc gggcccggtc cagtgtcgca taggcaatcg tggacagcgg cacgaaaatc
     2941 agccccagtc ccataccctg caggaatgca ggccagatga cccacatcgt gcttacatcc
     3001 agggaatacc aggtcatcgc ataagaaccg gcggcgctga tcagcatgcc gcttcctacc
     3061 aacaggcggg catccgcccg gttcaccaga cgcccgacga caatcatgct catggctgtt
     3121 gcgaggccgc gcggtgccat gacaaaaccg gtggtcacga tcgggtaatc gagcagccct
     3181 tccagcagaa tcggctgcaa gacaagcccg ccgaacaggc ccaatccaag ggcggccatg
     3241 accaggcagg ccatcgcaaa attccggtcg cggaaaatat gcaggtcgaa cagcgcgttt
     3301 tcgccgcggc gcagactgtg ccaaatgaag ccggaaattc ctacgatggc aagtgcagcc
     3361 gcgacgcgaa tgtcgatggc cgcaaaccag tcctgcgtgg tgccgcggtc cagcatatat
     3421 tgaagcccgg ccaccccgct gccgagcaag gccaatcccg cccagtccat gcgcctgctt
     3481 ttgcgtggcg cattgtggac atattgcgag gcgaggaaaa atgatagcgc gccgatcggc
     3541 acgttgatgt aaaaggtcca gcgccagctg gcgtattcgg tcagccatcc gccgagggtt
     3601 ggaccgagca ctggtccaac catgactccc aatccccaga tcgccatggc tttgccacgt
     3661 tcttcaacgg gataagcttc agtcatgatc gcctgcgaca atggcaccag tgcggcaccg
     3721 aaaacgcctt gcagcagccg gaatccgaca atctcggcca gcgattgaga gatgccgcag
     3781 agcgccgatg cggcaacgaa gccggcgatg caaacgagca ggaaacgctt gcgtccacgc
     3841 gtgtcggaaa agtaacccgt cagcggcatg acgatcgccg aggaaaccag gtaactggta
     3901 agcacccagc tgatctggtc agtggtcgcc cccaattctc cagccatgtg cggcaaagcg
     3961 acgttcacaa tggtggtatc gaggacctgg atcagcgtcg ccgacatgac ggcgagcgtg
     4021 atcatcagac gctcgccgcg catcattccg caccggaagc cgtcgcgacc aggatgctgc
     4081 aggatacgcg ctcgagcaac gactgctctt ctgactcgct ccaccagcgt gcgaggcgtc
     4141 cgcgcggttt gtggcccagc acgatcaagt ccgcatcgat gcgttgcgct gttgccgcga
     4201 tgcaatccgc ggcatttcca tattcgagcg atccttcggc tgctacgccg cgctcgcgca
     4261 gccaggcaat gccttcctgg agcagcgcgt ccgcgcgctc ctcttcctgc gcgcatagtt
     4321 ccggcgtgac gccctcgggt attgcggtcg aaacgagatt gcgcgaaata gccagcaagt
     4381 aaacttccga gcgcatgcaa atgacaacat ccgcgccttg ccgcaatgcg ttgcgtcctt
     4441 cgcgcgtgcc gtcatagcaa agtagcaccc gatgaaacat ggatcaccct gatttgacca
     4501 ctttcacggt tgcggtggtg ccaatgcgca gcggatgctg agggtcgggg tcttcgactt
     4561 taatgcgcac cgggacacgt tgcgtaacct tgacccagtt accggtcgcg ttttgtggcg
     4621 gcaacaggga gaacgccgtg ccgctaccgc cgctcaggct ttccacgacc ccatgaaata
     4681 cgcgatccgg ataaacgtcg gagctgatga ttgctttctg gccgggatgt atgtcgacaa
     4741 gctcggtttc cttgaagtta gcgtcgatcc agtattcctc atccgagatc accacgaaaa
     4801 ctggcacgcc gggttgcact gtattgcctg gctgcagggt caaattggcg accacgccgt
     4861 tcacggggga ggtcagccgc gttcgttcga ggtccagttg cgcctggcgt accgcggtaa
     4921 tcgcggactg cacgtttgca ttttgatcac ctaggcgacc gagcgcactt ttagcctggg
     4981 cgacgtttgc ctctgctgcc ttgaccgcag cttcggccgt ggccaattcg gtgcgggagt
     5041 tttccgcacc ctgcggcgat acaaaaccgc ttctcatgag tttctggttc cgattccagt
     5101 tgcgccgcgc attcgccgcc tcggcgcggc gttgcgccag caaggcttgc gcggaagcca
     5161 cgctggcgct ttcctgggag accgactggc gcgcaagctc aagttgtgct tgcgccttct
     5221 gcagcgccaa ctcgtaattt gcgggatcga tgtcgaacag cgggtctccg acttttactg
     5281 cctgttgatc ccgcacatac acttttatta ccggacccga aatttgcgcg gtgacttcca
     5341 cctgattggc ctgcacatag ccgtcatccg tgctgacata gcgcgaaatt tcacgccaat
     5401 acgcgattgc cgctatggcg agaaccacca gcacgatgcc cgcaatgatc catttgagct
     5461 gcttgcgtcc gagccgatgg ggaacgagta cgggcgcgga atgagcaggc gttgccgagt
     5521 catcgattgc ggaagatgcc atgcacccgg gacagcatgc gtagcgtttc gttcggacag
     5581 tgctgcctga accgctggcg actacggcgc gcgacgatcg ccatagggcg aatggatcag
     5641 tccacggttt gaccgcgtcc gcgctcaggc gcgcattgca tacgaatatg cacaaagaat
     5701 acttgcttcc tctattccgg tctgccgcac gtcgttgctg caaagtcgca cggcgcgatc
     5761 gatgacgatg tgctttttac cgatcgctgc agcggtttcg taaaaattac ttcggcgaac
     5821 aaccgaactt cggttgccct gctggcaacc cgcatgcgct tatccgtcgg catattgttt
     5881 gcgttgctgc tttcggtgat ggattgcatg ggcgaaactt ggctccggca gtccgtttgc
     5941 tggcccggga ctgccgtgga gcgaccatca gcaatggaag atcatgagga aaaattgtcg
     6001 ctgcgccagg cggccatttt tttgctggag gaatgcagaa ccatactgcc cggcatccag
     6061 gctttgttcg gctttcagct gatagcaata ttcaatgatg gctttgacac gaagctttct
     6121 tcatctgaac aggatcttca tcttgccgcc atttcgctgg tcgctgtcgc agtcgtgctg
     6181 atcatggctc cggccgctta ccatcggcag accgggccaa tgaaagtaaa tgcaaggttc
     6241 attaccgtgg cgagccgcgc cgccttgctg gccatgattg cactcatgct gggattgaca
     6301 ctcgatttct atgtcgttgc acaggtcgtt cttggcgaaa gaaaatggtc gctgttgctt
     6361 gccgcgctgc tatgcggcct tatcgttacc gtatggttca tgttgcccag aacgcgtgca
     6421 cttcgccggt atgccagcgc cggggaccat ctgcatgaac ctgcgaagga ccaacgaggt
     6481 gcttcaacgc atcatcggga ctaagcctgc gaccagattg cctggacacc agcgcgatga
     6541 attttgagcc cgtaccaaga caaagatata aatcggttcg cattttttac aaatgcaccc
     6601 gtaatcttgt accgctatct cgtacagggc aggttccgcc gctacgcaat taattcaaat
     6661 aattcataat tcaaataatt caaaaatgag tcatttagcg caggcaaatt tccttggcat
     6721 aagcattgca cgcccttcca cttccgacgg ctaaccgagc cgtctcatca tcacaaaaga
     6781 ggagatgcgg caatgaacaa actgctagcg gcactactcg tcagcgcgat ggcactgagc
     6841 acccc
//
LOCUS       DATMZQ010000382         5059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_648744, whole genome shotgun sequence.
ACCESSION   DATMZQ010000382 DATMZQ010000000
VERSION     DATMZQ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648744"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VN496_12335"
     CDS             complement(<1..264)
                     /locus_tag="VN496_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HXF17790.1"
                     /translation="MENIIRNEARRDFLKVSGSVAGGLALGFYLPGKHNVAVADAGGG
                     YASPNAWVKIGADNASTIMVARSEMGQDVYTSMPMLVAEELEVD"
     gene            complement(279..728)
                     /locus_tag="VN496_12340"
     CDS             complement(279..728)
                     /locus_tag="VN496_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXF17791.1"
                     /translation="MKISFNVNGKSTSVDVAPDTPLLWVLRDTLGMTGTKFGCGKALC
                     GACTVHLNGQAVRSCQTPVGSVSGQKVTTIEGLGGNHKVQKAWVALDVPQCGYCQPGQ
                     MMSASALLASNKNPSDEDIDAFMSGNICRCGTYPRIRAAIKQAAKMA"
     gene            complement(985..2217)
                     /locus_tag="VN496_12345"
     CDS             complement(985..2217)
                     /locus_tag="VN496_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF17792.1"
                     /translation="MNFEYSDKVKELQKKVSDFMQQHVMPQEAPFLEEIAQNRAKGNA
                     WIPTRIIEELKPKARAAGLWNLWQPKDHGGSLTNLEYAPLCEIMGRSSWAPEVFNCSA
                     PDTGNMEVLLRYGSPEQQKQWLIPLQNGEIRSAFLMTEPEVASSDATNIQCRIERQGD
                     AFIINGRKWWSSGAGDPRCKIFIVMGKSNPDSADRYKQQSMILVPADTKGVKIIRSLS
                     VFGYDDAPHGHMEVELDNVRVPASSMLLGEGRGFEIAQGRLGPGRIHHCMRIIGQAER
                     ALELMCKRLKSRVAFGKPVIEHNVWHERIAESRVMIEQARLLTLKAAYMMDTAGNKEA
                     KAEIAMIKVIAPNMAQQIMDWAIQAHGGGGVSQDFPLAMMYSHNRTLRLADGPDEVHR
                     NQIARLELKKWDHAKAHR"
     gene            complement(2438..2887)
                     /locus_tag="VN496_12350"
     CDS             complement(2438..2887)
                     /locus_tag="VN496_12350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXF17793.1"
                     /translation="MRQDFRFVHRLRVRWAEVDRQGIVFNGHYLTYFDIGVTEYYRAL
                     GYPYPSGLGLQGTDLYVRTAELEYHASAEYDDELDICVRVERLGRSSFDFRVEIYRTD
                     QCLVSGKLVYVNADPVARKSVRLPDFLRRAIKSFEHKAPVELENAKA"
     gene            complement(2898..3683)
                     /locus_tag="VN496_12355"
     CDS             complement(2898..3683)
                     /locus_tag="VN496_12355"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HXF17794.1"
                     /translation="MAERFPAIFVSHGAPTLPIERSAAREFLADLGSELGRPAAILVI
                     SAHWESGEAAISSTVQPETIHDFFGFPQELYRMVYPAPGAPQLASRAAALLQQDGIAA
                     KLHPSRGLDHGAWVPLMLMYPEADIAATQLSVQPEQGTNSHFELGEALRPLRDEGILI
                     LASGGATHNLYEFGRHRRDAAPPEWVTAFQEWLALTIERGNKSEFLGYRTNGPHAARN
                     HPSEEHFLPLFVAAGAGTSGTVGKRIHRSHSYGILAMDAYRFD"
     gene            complement(3676..4137)
                     /locus_tag="VN496_12360"
     CDS             complement(3676..4137)
                     /locus_tag="VN496_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXF17795.1"
                     /translation="MARAVFNSLDDLAGKIGVEVVVSDWLEVTQDRIQQFADATGDHQ
                     WIHVDVERARRESPFGAPIAHGFLTMSLLSHFLNESLEFGKSKMGVNYGFNRLRFTAP
                     VKVGSRLRARFTLKDFQRIEGGVQMVWDIVMESEGETKPVLVAEWVGRRYG"
     gene            4232..>5059
                     /gene="gnd"
                     /locus_tag="VN496_12365"
     CDS             4232..>5059
                     /gene="gnd"
                     /locus_tag="VN496_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXF17796.1"
                     /translation="MERKMQIGMIGLGRMGANMARRLARGGVHVGAFDVSPLARSALA
                     RESGVTPHAELSSLVAAMPAPRVVWTMLPSGEVTEQALQTLATLLATGDVLVDGGNAN
                     YKDSQRRATWLHERSIGFVDAGVSGGVWGLENGYALMLGGSKEAVAHVEPFVKLLAPA
                     ADRGWIHCGPAGSGHYVKMIHNGIEYGLMQAYAEGISMMKAKSEFDLDLAAITEMWRH
                     GSVVRSWLLDLTADFLRKGQALETIEPIVADSGEGRWTAIEAIELGVPAPVMSLALMA
                     "
BASE COUNT         1056 a         1471 c         1427 g         1105 t
ORIGIN      
        1 gtcgacttcg agctcttcgg ccacgagcat tggcatcgag gtatagacgt cctgccccat
       61 ttcggaacgc gcaaccatga tggtgctagc gttgtcggcg ccgattttga cccaggcatt
      121 cgggctggcg taaccaccgc ccgcgtcggc aaccgcgaca ttatgtttgc ctggcaagta
      181 gaacccgagc gccaatccgc cggcgacgga accgctgact ttcaggaagt cgcggcgagc
      241 ttcatttctg atgatatttt ccatttgtca tttcctcctt aggccatctt cgcagcttgc
      301 ttgatcgctg cgcgaatacg aggatacgtt ccgcaacggc agatattgcc ggacatgaac
      361 gcatcgatat cttcgtcgga gggatttttg ttggaagcaa gcagcgcgga tgccgacatc
      421 atttgaccgg gctggcagta cccgcattgc ggcacgtcca gggcgaccca cgccttctgc
      481 actttgtgat tgccaccgag gccttcgatg gtcgttactt tctggccgga cactgaaccg
      541 actggcgtct ggcaggagcg cacggcctgg ccgttcaaat gcacggtaca ggcaccgcaa
      601 agcgccttgc cgcaaccgaa tttggtgccg gtcatgccca gggtgtcgcg aagtacccag
      661 agcaacggcg tatccggcgc gacatccacg ctcgtgcttt ttccatttac gttgaacgag
      721 attttcattg caaagctccc cctttggtga ggaatgaagt tatgtatgag atagagacgg
      781 gcgtaacacg gagatagtta cgccggccaa aatacacgaa tcaaaagtgc gttacaactc
      841 tttggcttac atagtttcaa gcggcggcaa caggcctcga catcaacttt tgctttggtg
      901 cgtaaacccg cggcagcatt cacgattgca aggagcccct ggttaacgtg ctgcatagca
      961 atattcagcg aagaaacccg ctagtcaacg atgagccttt gcgtggtccc atttcttcag
     1021 ttccagacgc gcaatctggt tacggtggac ctcatcgggc ccgtctgcca ggcgcagtgt
     1081 acgattgtgg ctgtacatca tcgccagcgg aaaatcctga ctcacgccac cgcccccatg
     1141 tgcctgaata gcccaatcca tgatctgctg tgccatgttc ggcgcaatga ccttgatcat
     1201 cgctatttcg gcttttgctt ccttgttgcc agccgtgtcc atcatatagg ctgcctttaa
     1261 agtcagaagc cgggcttgtt cgatcatgac gcgggactcg gctatgcgct cgtgccagac
     1321 gttgtgttca ataaccggct tgccgaatgc cacgcggctc ttgagccgtt tacacatcaa
     1381 ttccagcgct cgctcggcct gaccgatgat acgcatgcaa tgatggatgc gcccggggcc
     1441 caatctgcct tgcgcgattt cgaagccgcg tccttcgccc agcaacatgc tggaagccgg
     1501 cacccgcaca ttgtcgagct ctacttccat gtggccatgc ggtgcatcgt catacccgaa
     1561 cacgctcagc gaacggatga ttttgacccc tttggtatcg gccggcacca ggatcatcga
     1621 ctgctgcttg tagcgatcgg cactgtctgg attggacttg cccatgacaa taaaaatctt
     1681 gcagcgcgga tcacctgcac cggaagacca ccacttgcgg ccattgatga taaatgcatc
     1741 gccttggcgc tcgatgcggc attgaatgtt ggtcgcatcc gaggaagcga cctctggctc
     1801 ggtcatcagg aaggcagatc gtatctcgcc gttttgaagc gggatcagcc actgcttctg
     1861 ctgctccggc gacccataac gcagcagaac ttccatgttt ccggtatcgg gtgccgaaca
     1921 gttgaacacc tcgggagccc agctgctgcg ccccatgatt tcgcacagcg gcgcgtactc
     1981 cagattcgtc aatgaaccgc catggtcctt cggctgccac aggttccata accccgccgc
     2041 gcgcgccttc ggcttgagtt cttcgatgat gcgcgttggg atccaggcat tgcccttggc
     2101 gcggttttgc gctatttcct caaggaacgg cgcctcttgc ggcatcacat gctgttgcat
     2161 gaaatcggaa actttttttt gcagttcttt cactttgtcc gagtattcga agttcatgtc
     2221 tgttccttgc gttggggttc ggaatagcgt tagaggcgaa catcgcccga atgcaaatcg
     2281 tgtgcggtta taaacgtgtc agacgatcaa ctggcaccgt tgcgtccccg gcgcgcgact
     2341 ggcgcaccaa gccgctcttt agcttcccgg tgaagatcga ggcctgcgta gccaaagggg
     2401 ctgcgctagc tccattatcg cactgcaggc gaacacttca tgctttggcg ttctccagtt
     2461 caaccggcgc cttatgctca aatgacttta tggccctgcg caaaaaatcc ggcagacgga
     2521 ccgacttgcg cgcaaccgga tctgcattga cgtataccag cttgccgctc accaggcatt
     2581 gatcggtccg atagatttct acccgaaagt cgaagctgga ccgcccaagg cgctcgaccc
     2641 gtacgcaaat atcgagctca tcgtcatact cagctgatgc gtggtattcc agctcagccg
     2701 tgcgaacata gaggtcagtg ccttgtaatc ccaggcctga cgggtaggga taaccaagcg
     2761 cacggtaata ttccgtgaca ccgatatcga agtaggtcag atagtggccg ttgaatacga
     2821 tgccctgccg gtccacttct gcccagcgta cgcgcaggcg gtgtacgaat ctgaaatcct
     2881 gtctcatacg cggcaattca gtcgaagcgg tacgcgtcca tcgcgaggat gccgtaacta
     2941 tggctgcgat gaatgcgctt tcccacggtc cctgatgtgc ctgccccagc cgcaacaaat
     3001 agcgggagaa agtgctcctc gctgggatgg ttccgcgccg catgggggcc gtttgtgcga
     3061 tacccgagaa attcactctt gttaccgcgt tcaatagtaa gcgccagcca ttcctgaaaa
     3121 gcagtaaccc attctggcgg tgccgcgtcg cgccgatggc ggccaaactc gtaaagatta
     3181 tgagtcgcac cacctgacgc gagtatcagg atgccttcat cgcgtaacgg tctcagcgcc
     3241 tcacccaatt caaaatggga attcgtgccc tgctccggtt gcaccgataa ttgggtagcc
     3301 gcaatgtcgg cctccgggta catcagcatt aacgggaccc atgccccgtg gtccagtccg
     3361 cgagaaggat gcagtttggc tgcaatgccg tcctgctgaa gcaacgcagc cgcgcgcgat
     3421 gccagttgtg gggccccggg cgccggatac accatgcggt acaactcctg cggaaaaccg
     3481 aagaaatcgt gaatcgtctc cggctgaacc gtggaactga tggcggcttc accgctttcc
     3541 cagtgcgcag agatgaccag tatcgctgca ggcctgccaa gttcgcttcc cagatccgcg
     3601 aggaattctc gggcggcact gcgctcaatg ggcaacgtcg gagccccgtg tgaaacaaaa
     3661 atcgcgggga accgctcagc catagcgccg acccacccac tcggcaacca gcacaggttt
     3721 ggtctcgcct tcactttcca tgacaatatc ccaaaccatc tgtacgcctc cttcaatccg
     3781 ctgaaaatct ttcagcgtga agcgggcgcg caggcgagag ccaaccttta ccggcgcggt
     3841 gaagcgcaat cggttgaatc cataattgac acccattttc gatttgccaa actcgagcga
     3901 ctcattgagg aaatggctca gcagcgacat tgtcaaaaac ccgtgggcga taggcgctcc
     3961 gaaaggcgat tcacgccgtg cgcgttcaac gtcgacatgg atccactgat gatctcctgt
     4021 cgcgtccgcg aattgctgaa tgcgatcttg tgtgacttcg agccagtcgc ttacaacgac
     4081 ttccacgccg attttgcctg ccaggtcatc caggctgttg aacactgctc gcgccatccc
     4141 cgcccccttt gggcggcaat gtaccggatt caaggtacgc gcgcattgcg cgtcgcgatt
     4201 cgagccggga caatgatccc tcgggatgac gatggagaga aaaatgcaga tcggcatgat
     4261 tggcttgggc cgcatgggcg cgaatatggc gcggcgcctg gcacgtggcg gcgtgcatgt
     4321 gggcgcgttt gacgtaagcc cgctggcgcg tagcgcgctc gcacgggaga gcggggttac
     4381 gcctcatgca gagctgagct cgctggtcgc cgcaatgccg gccccgcgtg tcgtatggac
     4441 catgctgcca tccggtgagg tgaccgagca ggctctacag acgttggcaa ccctgcttgc
     4501 aacgggagat gtgctggtgg acggcggtaa cgccaactac aaagattccc agcgacgcgc
     4561 cacgtggttg catgaacgca gtattggctt cgtcgatgct ggcgtgtccg gtggggtctg
     4621 gggcctggaa aacggatacg ccctgatgct gggaggaagc aaagaagcgg tcgcccatgt
     4681 tgaaccattt gtgaaactgc tggcgcccgc ggcagatcgt ggatggattc attgcgggcc
     4741 ggccggttcc gggcattacg tcaagatgat ccacaacggc atcgaatacg gactgatgca
     4801 agcctatgcc gaaggaattt caatgatgaa agcaaagtcg gaatttgatc tggacctcgc
     4861 cgcaatcacg gaaatgtggc gtcacggcag tgtagtgcgc tcatggctac tggatctgac
     4921 agccgatttc ctgaggaagg gacaggcgct tgagacgatc gagcccattg ttgccgactc
     4981 gggagaaggt cgctggacgg cgatcgaagc gatcgaactc ggcgtgccag ccccggttat
     5041 gagcttggcg ctgatggcg
//
LOCUS       DATMZQ010000383        14575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2718573, whole genome shotgun sequence.
ACCESSION   DATMZQ010000383 DATMZQ010000000
VERSION     DATMZQ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14575
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718573"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VN496_12370"
     CDS             <1..771
                     /locus_tag="VN496_12370"
                     /inference="COORDINATES: protein motif:HMM:NF018535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HXF17797.1"
                     /translation="TKELDANDAAAVTVLMGGAARTRLVPTDYLCDDLYPAFRHAARN
                     WVVGVQSGVLPDLRDQHNRLGTRLLAHGLTPSPPTNARDDLPAVLQEILPAGIDLHRM
                     DGVLFGRSQTSPNAFFGLIDGTPVGALTVWTLAGECLALADERPGEPVTVFLDCPGQA
                     PTFQDELVMLSEYVAHLALVLSTLRRNGHRVLLQVLGDAAGGIYVALAAPATRVLALP
                     GANVQVLPPAAIVRVLRRRSPSPDVEDYLRAGVVDTII"
     gene            779..2293
                     /locus_tag="VN496_12375"
     CDS             779..2293
                     /locus_tag="VN496_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HXF17798.1"
                     /translation="MNQNFFAAVAAAIGCDAEQVLLETDDGKLYTRGDILRESARLAG
                     LLCDAGAVRGDRIAVQVDKSPAALSLYLACLRAGLVYLPLNIAYQRAELAYFLQDARP
                     RVVVCRPESSPLLGELSAGRAEVFTLDHQGRGSLTDAAASLPHEFKTADCATDDLAVI
                     IYTSGTTGRSKGAMVTHRNLLSNARALVDYWGFSDRDVLLHALPVFHIHGLFVANHCA
                     LLSGAKLLWHRKFDPVLVLRDLARATVMMGVPTFYTRLLAHPGFGRDACESVRLFISG
                     SAPLQLETFKEFKERSGHSILERYGMSEAGMICSNPLHGERRAGSVGLPLPGVSVRIA
                     QDYDIPVAPGAVGGIQIKGENVFAGYWENPDKTRLEFTDDGYFRTGDLGAFDSDGYLS
                     IVGRAKDLIISGGYNVYPKEIEIALDALPGVMESAVFGIPHADFGEAVAAVLVPKQGV
                     LLDTTAMIVELRKNLANYKVPKQILLVEELPRNAMGKVQKALLRERYAKQITAG"
     gene            complement(2345..3142)
                     /locus_tag="VN496_12380"
     CDS             complement(2345..3142)
                     /locus_tag="VN496_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXF17799.1"
                     /translation="MDLWWMVYLLMGLFVGFFAGLLGIGGGLMLVTLMVYLFTLQGFP
                     ADRLLHLALGTSIASIVFTSLASLRAHQKHGAVRWDILRAAVPGLVVGTLLGAVVADQ
                     LKSKYLAIFFVIFVYYSAVQMFANVKPKPTRQLPDKPGMTVAALVIGIVSSLVGVGGG
                     VMTIPFMSLCNVPMRQAIGTSAALGLPIAIAGTVGFIVTGLGKDHLPALSVGYVYLPA
                     LIGIVLGTFITVPWGARLTHRMPVSTLKKTFAVILFILATRMLWSLF"
     gene            complement(3152..3607)
                     /locus_tag="VN496_12385"
     CDS             complement(3152..3607)
                     /locus_tag="VN496_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXF17800.1"
                     /translation="MNYWLMKSEPSVFGIDDLAQAPGKTAAWWGVRNYQARNFMRDQM
                     RIGDRVFFYHSSCPEPGIAGIARVSKRAYPDETQFDRTSEYFDPKASRDHPRWFNVDV
                     TLVRKTRVIGLPELRSHPQLASMRVLQRGNRLSITPVDPNEAAFVMKLF"
     gene            complement(3906..4220)
                     /locus_tag="VN496_12390"
     CDS             complement(3906..4220)
                     /locus_tag="VN496_12390"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXF17801.1"
                     /translation="MSAQTKGGLDVNIMGREFRIACQEDEQKDLLKAIEYLNIKMREI
                     RDAGKVIGVERVAIMAALNITHEFLSSRMDGGFDVGEFKRRIFRMQTTIDQAMNGQDE
                     LF"
     gene            complement(4217..4417)
                     /locus_tag="VN496_12395"
     CDS             complement(4217..4417)
                     /locus_tag="VN496_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17802.1"
                     /translation="MEAELTALDDKISQLIKLTQKLREDNSQLRQNLTSVQSENKRLA
                     DKVNIAKTRLETLLTQIPDGAE"
     regulatory      4602..4833
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4970..7036
                     /locus_tag="VN496_12400"
     CDS             4970..7036
                     /locus_tag="VN496_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXF17803.1"
                     /translation="MLAGALVSTNASAQKSQELVAEEVVVTATRFDDLSVDKPLSITT
                     ITSDEIRRSPARTVPELLSYQVGIGGRDLFGNNAAQATVDMRGFGATAAQNTLVLLDG
                     RKMNDIDLSGVQWSALPLDSIDRIEIIRGSGAVQYGDGAVGGVINIITRKPGKDGPQA
                     TIAAGVGSFDTYQARASGELGSDNAGLRLGGAYYDSNGYRDNNRNIQSTASAEGRWKG
                     SIGDISLRFGGASENLRLPGARTVQPSIGLNELETDRTGTSTPKDYALRDDNVLQFDW
                     SRADGAISEVVGVSYRKKEQKSYFDFNGFPDYREIDLDVLALTPRMRFDHSQLGLNGN
                     FVVGFDWYTWNYDLALSASPTTIAQPVHKVDARQENRAIYVLESLKPTPRMTLTGGAR
                     VEWFDIDASDTFDATAPNPTFINGGSPSGNQSDRQFAWEVGLRQALTEQWSGYTRATR
                     AYRFATVDETYETTALFEQSFQFLRPQTSITYEVGVDWRASMTDARIGVFQTDVKDEI
                     HLDAFTSGIGNTNLPPSRRRGVEFEMGVQPAERVDLRLTYTYTDARFLEGEFPGGPFS
                     ITNVNIVGKTVPLVPRHKATLNGNWGITPSTNLSGAVVYVSEQFMDNDEANNLGVKIP
                     GYTVVDFKLTQQVGAARFGVAVNNALNEKYFNYAVRSQFTPDRYNAYPLPERTFMAFV
                     EYKFGK"
     gene            7121..8899
                     /gene="msbA"
                     /locus_tag="VN496_12405"
     CDS             7121..8899
                     /gene="msbA"
                     /locus_tag="VN496_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HXF17804.1"
                     /translation="MQEMTGTELYFRLLRYVKPYWGVFALSILGMLITAATEVALPVA
                     VKPFLDGTFVKKDPFLVTWVPVLMVLLYVIRGAGAYIGTYASTWVGNKVVMDLRDQMF
                     RRMLALPLGYFHDNTTGNLISRFTFDAAQVTGAATNVVTVLVKDSVTILGLLTYLLYI
                     DWKLTLISLIMVPPIAVVVRYFNVRLRNMSRKTQEAMGDITQVLQETIECNKVVKIFG
                     GQAYEAKRFADTSNRLRGFTMKQTAAAAANVPIVQLLAAIAVAVVVYYATVQAQSDAT
                     TVGGFVSFLAAMLLLTPPLKRLTGVAEYLQRGLAAAESVFALLDEKPETDTGKIPLQR
                     ARGDIAFRQVNFRYARAEHSALRNVTLSISAGETIALVGSSGGGKTTFANMVPRFYHP
                     DSGTITIDGHDIEALSLADLRRNIALVSQEVALFNDTVSANIAYGQAAGASRHAIERA
                     AEAAHALEFIQAMPHGFDTLIGENGVRLSGGQRQRLAIARTVLKDAPILILDEATSAL
                     DSESEKHVQAALEQLMQGRTTIVIAHRLSTIENADRIVVLDNGEIAQIGTHAQLLAEG
                     GIYAKLYRIQYAAGGTQPEAELHSVA"
     gene            9047..9250
                     /locus_tag="VN496_12410"
     CDS             9047..9250
                     /locus_tag="VN496_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXF17805.1"
                     /translation="MAVCPYNVFRIATVTADLRRGLSLRGKLKGYAHRWQQAFAANAD
                     ACHACGLCVAACPEKAITLVRSI"
     gene            9915..10526
                     /locus_tag="VN496_12415"
     CDS             9915..10526
                     /locus_tag="VN496_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXF17806.1"
                     /translation="MMRRVEQLPTSPVRRAAAALVKVIPMLVGATLLSACQHGPTVFD
                     DPSAIAVLAATPGSAVHGVVTFIRAGEATLVNANLSGFKPNSTHGMHIHVIGDCSAHD
                     ASSTGEHFNPQSSRHGAPGAAEHHAGDLGNITADAVGNVYATFKITEGSFGTGDDSII
                     GRGLVVHADRDDLNSQPAGNSGVRLACGSITRNPDRMTVAKSG"
     gene            complement(10536..13247)
                     /gene="polA"
                     /locus_tag="VN496_12420"
     CDS             complement(10536..13247)
                     /gene="polA"
                     /locus_tag="VN496_12420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXF17807.1"
                     /translation="MKTLVLVDGSSYLYRAFHALPDLRTRQGEPTGAVKGVLSMLQKL
                     RNETSADYIACVFDAKGKTFRDDWYPQYKATRATMPDDLARQIEPLHDSVRAMGWPLL
                     MVDGVEADDVIGTLAKQAEARGVHTIISTSDKDITQLVNPMVSLVNTMSSESLDEKGV
                     EQKFGIKPAQMVDYLTLIGDSVDNIPGVDKVGPKTAVKWLLLYGSLDEVIRHADEIPG
                     AVGENLRKALPWLPTAKKLLSIKCDVPLPVDVEQLAPQPHNVPELRRLFEKLEFRGLL
                     RELQDKALGTADQDTKETAVSRPHTHQRTYETILTDEQLRAWIAKIKPAPLVSLETET
                     TTLDPMTTHLVGIALSIEAHGGAYIPVGHAYAGAPMQLSREHVLTALKDWLEDEGARK
                     LGQNSKYDRHVFANCGIKLAGVEHDITLQSYVLESHRPHDMDNLASRHLNMTTTTYDD
                     VTGKGAARISFEQVELTRAAEYAAEDADVALQLHQVLHPQIEQDAKLLRIYREIEMPV
                     SQVLFAMERHGVLLDAQLLEQQSRELGIKLMEIEQSVHLQAGQPFNLNSPKQLQEILF
                     ERQGLPVIKKTPSGVPSTDEDVLQQLALDHPLPKLILEYRGLSKLKSTYTDKLPRMVN
                     AETGRVHTNYAQAVAVTGRLSSNEPNLQNIPIRTAEGRRIREAFVAAPGSVIVSADYS
                     QVELRIMAHLSQDKGLLHAFAAGEDVHRHTASEIFGVDSAAVTSEQRRYAKVINFGLI
                     YGMSAFGLATQLGIERAAAQQYMERYFLRYPGVRNYMERTRETARKLGYVETVFGRRL
                     YLPDINASNGPRRQGSERAAINAPMQGTAADLIKLAMIAVHRWISSTGRKSRLVMQVH
                     DELVLEVPEAEIDVIKTGLPQLMNSVATLDVPLAVDLGVGPNWDKAH"
     gene            13272..14000
                     /locus_tag="VN496_12425"
     CDS             13272..14000
                     /locus_tag="VN496_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXF17808.1"
                     /translation="MSEKQKVPVPLAPEFAEKAWSARESWRVFGIMAEFVEATERLAH
                     IRPAVSVFGSARTRPDHGYYALGEAIGRKLSDAGFAVISGGGPGIMESVNKGAYAGRS
                     LSVGLNIQLPHEQSANAYQDVSQTFRHFFARKVMFAKFACAYVVLPGGFGTLDELGEI
                     LTLVQTGKIRRIPIILVGGAFWRGLLDWIADRMVSEAMINPEDMDLIQMIDDPGYVVD
                     AIFDHYEKRGFELTAAEREAQLNL"
     gene            14010..>14575
                     /locus_tag="VN496_12430"
     CDS             14010..>14575
                     /locus_tag="VN496_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXF17809.1"
                     /translation="MSVFTTVTREQLSAWLKNYSIGVPTSLEGILSGIENTNYFVTTT
                     HGRFVLTLFEKLTRNELPFYLNLMAHLSSHGLPCPKPIANLQNQLLGELNGKPAAIVS
                     CLEGQPVTDPAPLHCARVGEVLAEMHLAGRTYQGQLDNLRGAEWWTACAPEIYPFLKP
                     EDAELLKSEIHYQQRQNRKGLPRGVVHA"
BASE COUNT         3108 a         4068 c         4149 g         3250 t
ORIGIN      
        1 accaaagagc tagatgcgaa tgatgctgct gctgtcaccg tcctaatggg gggagcggcc
       61 cgcacgcgcc tggtgccgac agattatttg tgtgacgact tgtaccctgc gtttcgccac
      121 gccgcgcgta actgggtggt cggtgttcaa tctggcgtgc taccggacct gcgcgatcag
      181 cacaaccgtc ttggcacgcg tctgcttgcg cacggtctga cgccgtcacc gcccactaac
      241 gcgcgcgatg atctaccagc ggtactccag gaaatcctgc cggcagggat tgatttgcac
      301 cgaatggacg gtgtgctttt cggtcgtagc cagacttcgc caaacgcatt ttttgggctt
      361 atcgacggca cgccggtcgg tgcattgact gtctggacgc ttgcgggcga atgccttgcg
      421 cttgctgatg aacggccagg cgagccggta acggtttttt tggattgccc gggccaggca
      481 cccacttttc aggacgaact cgtcatgttg tccgaatatg tggcgcatct ggcgctggtc
      541 ctttcaacct tacggcgcaa cggccaccgc gtactgcttc aagtattggg agatgccgcc
      601 ggcggaattt acgttgcctt ggcggcgcct gctacgcgcg tacttgcatt gccgggtgcc
      661 aacgtacagg tcctgccgcc ggctgctatc gtgcgtgtgc tgcgtcggcg cagtccgtcc
      721 cctgatgtgg aagattacct tcgcgcgggc gtagtcgaca cgattatatg agctgccgtt
      781 gaaccagaat ttttttgctg ctgtggcagc tgccatcgga tgcgacgcgg aacaggtatt
      841 gctggaaacc gatgacggca agctctacac gcgtggcgat attcttcgtg aatcggctcg
      901 tcttgccggc ttgctgtgtg atgccggcgc ggtgcgaggc gatcgcattg ccgtgcaggt
      961 ggataagtct ccggctgcgc tttctctcta cctggcttgc ttacgtgccg ggctggtcta
     1021 tctgccactg aacatcgcgt atcagcgtgc cgagctggcc tatttccttc aggatgcgag
     1081 accccgggtg gtcgtgtgtc gaccggaatc atcgccattg ctaggcgagc tttccgcggg
     1141 acgagcggag gtttttacgc tggatcatca ggggcgcggc tctcttacgg acgccgccgc
     1201 ctcgttgccg cacgaattca aaacggctga ctgtgcgact gatgatttgg ccgtcatcat
     1261 ctatacctcg ggcacgaccg ggcgctccaa gggagcgatg gtgactcatc gcaacctgct
     1321 ttcgaacgcg cgcgcgttgg tggattattg gggattcagc gatcgtgacg tgctcttgca
     1381 cgccttgcct gtgttccata tccatggatt gttcgtcgca aaccattgcg cgttgttgtc
     1441 cggcgccaag ctgctgtggc atcgcaagtt cgatccggtg ctcgtattgc gggatttggc
     1501 acgcgcgacc gtcatgatgg gcgtacctac gttctacacg agattgctgg cgcatccggg
     1561 attcggtcgc gatgcgtgcg aaagcgtgcg tttgttcatt tccggttctg caccgcttca
     1621 actcgagacg ttcaaggaat tcaaggaacg cagcgggcac tccattctgg aacgctatgg
     1681 catgtccgag gccggcatga tctgttccaa tccgctgcac ggagaacgcc gcgcaggcag
     1741 tgtcggtttg ccattgcctg gtgtatcggt aaggatcgcg caagactacg acatcccggt
     1801 cgcaccgggt gcagtcggcg gaatacagat caaaggggag aacgtattcg ccggttactg
     1861 ggagaatccc gacaagaccc ggcttgaatt taccgatgac ggatattttc gcaccggaga
     1921 tcttggcgca ttcgatagcg acggatattt gtcgatcgta ggccgtgcca aggatctgat
     1981 catcagtggg ggatataacg tttaccccaa ggaaattgaa attgcgcttg atgcgctgcc
     2041 aggcgttatg gagtctgccg tttttggcat tccgcatgct gattttggcg aggccgttgc
     2101 ggcggtgctg gtgccaaagc aaggagtgct gcttgacacg acagccatga ttgtcgagct
     2161 aaggaaaaac ctcgctaatt ataaagtgcc gaagcagata cttctggtcg aggagttgcc
     2221 gcgcaacgcg atgggcaaag tgcaaaaagc cttgttgcga gagcgttatg ccaagcagat
     2281 cacggcagga taaatattgg gcgatgaacg aacggaccgg caatagtttg ttatcgcgag
     2341 cctatcaaaa tagcgaccac aacatcctgg tggcaaggat aaacagaata acggcaaagg
     2401 tctttttcaa ggtactgacc ggcatgcggt gggttagcct tgcgccccat ggcaccgtaa
     2461 tgaacgtacc cagcacgata ccgatcaggg caggcaaata gacatatccc acgctaaggg
     2521 ccggtaagtg gtctttgccc aatcccgtca cgatgaagcc cactgtaccg gcgatggcta
     2581 tcggcaggcc gagcgccgct gaggtaccga tggcctgacg catcggaacg ttacaaaggc
     2641 tcatgaaagg aatggtcatt accccaccgc cgacgcctac caggctggaa actatgccga
     2701 tgacgagcgc tgctactgtc attcccggtt tatcgggcag ttgccgtgtc ggcttcggct
     2761 tcacgttggc gaacatctgt actgcagagt aatagacaaa aattacgaaa aatatggcga
     2821 gatatttgga tttcagttga tcggcgacga cagcgccaag caaagtaccc acaaccaacc
     2881 ctggaactgc tgcgcgcaaa atgtcccagc gcactgctcc atgcttctgg tgagcccgca
     2941 aactggcaag cgacgtgaag acgatgctcg caatcgaagt ccctagtgca aggtgcaaca
     3001 ggcgatctgc tggaaagccc tgcaaggtga ataaatacac catcagtgtg accagcatta
     3061 agccgccgcc gataccgagc agccctgcga agaagcccac gaacagtccc atcaacagat
     3121 agaccatcca ccacaaatcc atggcggccg cctagaacaa tttcatgaca aacgctgcct
     3181 cgtttggatc aaccggtgta atcgatagcc gattgccccg ctgcagcact cgcatcgatg
     3241 ccaattgcgg atgggagcgc aactcgggaa gaccaatcac ccgtgtcttt ctgaccagcg
     3301 tcacatcgac attgaaccaa cgcggatggt cgcgggacgc cttcggatcg aagtattcgc
     3361 tggtacggtc gaattgggtt tcgtctggat aggcccgctt tgaaacgcgt gcaatgcctg
     3421 caatgccggg ctctgggcaa ctcgaatggt aaaaaaatac gcgatccccg atgcgcattt
     3481 ggtcgcgcat gaaattacga gcctgatagt tgcgcacgcc ccaccatgca gcagttttcc
     3541 ccggagcctg agccagatca tcaatgccga atacactcgg ctcggatttc atcagccaat
     3601 aattcatgtt tcagtgacgc aaaaaaaagc tccggggtcc ggaacttttg gtatggcaaa
     3661 tggcgcaagg catagggcgc gaagccacct cggtacgatt gtgactgcgc aagatgggga
     3721 gccccccgcg attcccgtca ggccggcatc ttgaacctag ggtccaaggc ggtcgcattc
     3781 cgagcacatc aggcgcttcc gattgggacg cgcacaacag cgctacttta ggcagcaacc
     3841 aagtcgatta acattggttc aaagaattac aacctgacga acgccgcagg ggacattgac
     3901 tcgcatcaga acaactcatc ctgaccattc atcgcctggt caatcgttgt ctgcatgcga
     3961 aagattctac gcttaaattc gccgacatca aacccgccgt ccatcctgct ggaaaggaat
     4021 tcgtgtgtaa tattcaacgc cgccatgatg gctacgcgtt ccacgccgat gactttgccg
     4081 gcatctcgaa tttcgcgcat cttgatattc aggtactcga tagccttcag caagtctttt
     4141 tgttcgtctt cctggcacgc aatacgaaac tcgcgtccca tgatattcac gtcgaggccg
     4201 cctttggtct gggcactcat tctgcgccat ccgggatctg ggttaacaaa gtctccaggc
     4261 gcgtcttcgc gatgtttact ttatctgcga ggcgcttgtt ctcgctttga acagaagtca
     4321 aattctgccg cagctgggag ttatcctcgc ggagcttctg ggtcagcttg atgagctgac
     4381 tgatcttgtc atcgagcgcg gtaagttcag cttccatgag gacactataa ttatgaaaac
     4441 cgtgaaaagt caactgctaa cgcatttttt tgaaagtgct ttggcatatg tggccgatcc
     4501 ctgccctccc tcggcttaga gcggtctgcc agcgatccgt tgtccgtgcc agacatgtaa
     4561 ttgacattaa tcccgatgtt gggattccgg ggatcggcta gaatcccggc acgttctcgg
     4621 gtgccccgaa cagttccagt aaggggtcaa acgggaaaca ggtgagcgac tttaaaatct
     4681 tttaggcgca attcctgtgc tgcccccgca acggtaagcg actggtgggg cgtatcaaga
     4741 tgccactggg gaggccggga actttccgtc cccttgggaa ggcgatacgt ccaagcgcaa
     4801 gcccggatac cggcccctgg atgcaacgga gatccgcgtg gggcgggcat tccggtgtcg
     4861 gcatgtcctt caccctgctt tccctgtggc tgaactccct ttttaaattc gcgcatacgg
     4921 cgggtttgcg catcaagggg cagccatgaa actgatgcgg gtacacctgt tgttggccgg
     4981 cgcgctagtg agcaccaacg cctctgcgca aaaatcgcag gagcttgttg ctgaagaggt
     5041 ggtggtgacg gcgacaagat tcgacgatct ttctgtcgat aaaccactca gcataaccac
     5101 catcacttcc gatgagatcc ggcgatcgcc ggctcgcacc gtaccggaac tgctgtccta
     5161 ccaggtcggc atcggcggcc gcgacctttt cggcaacaac gctgcgcagg cgactgtcga
     5221 catgcgcggg ttcggcgcga ccgccgctca gaacacgctg gtgctgctcg acggcagaaa
     5281 aatgaacgat atcgaccttt cgggcgtgca gtggtcagca ttaccactgg attccatcga
     5341 ccgtatagaa atcatccgcg gcagcggcgc cgtacagtat ggggacggcg cggtcggcgg
     5401 ggttataaac atcatcacgc gcaagccggg aaaggatggc ccgcaggcga cgattgcggc
     5461 tggcgtcggc agtttcgaca cctaccaggc tcgagcgagc ggcgaactgg ggagcgataa
     5521 cgctggcctg cgcctaggtg gcgcctacta cgattccaat ggctaccgcg acaacaatcg
     5581 taatatccag tcgaccgctt cggccgaagg acgctggaag ggttcgatcg gagacatctc
     5641 actgcgtttt ggcggtgcta gcgagaatct gcgcctgccg ggcgcgcgca ccgtccaacc
     5701 gagcatcgga ctcaacgaac ttgagaccga ccgcacagga acgtcgacgc cgaaggacta
     5761 tgcgttgcgg gacgataacg tgctgcaatt cgattggtcg cgcgcggatg gtgcaatctc
     5821 cgaggtggtg ggcgtgtcct atcgcaagaa ggaacagaaa tcgtattttg acttcaacgg
     5881 atttcccgac taccgcgaaa tcgatctcga cgttcttgca ttgacaccgc ggatgcgttt
     5941 cgatcattca caactcgggc tgaacggcaa ctttgtcgtc ggcttcgatt ggtatacgtg
     6001 gaactacgat ctcgccctgt ccgcctcgcc gaccaccatc gcccaacctg tacataaggt
     6061 cgatgcccgg caggaaaacc gtgcgattta tgttctggaa tcgcttaaac cgacgcctcg
     6121 catgacgctg accgggggag cccgcgtcga atggtttgat atcgatgcga gcgatacctt
     6181 cgacgcaacg gcaccaaatc caacttttat taatggcgga tcgcccagcg gcaaccagag
     6241 cgaccgccag ttcgcatggg aagtcgggct ccggcaggca ctgaccgaac aatggtcggg
     6301 atacactcgc gcaacgcgag cctatcgttt tgcgactgtg gacgagacct acgagaccac
     6361 cgcgcttttc gagcagtcgt tccagttcct gcggccgcag acgtcgatca cctacgaagt
     6421 gggtgtcgat tggcgcgcgt cgatgacaga cgcacgtatc ggagtctttc agaccgacgt
     6481 gaaggatgag attcacctgg atgcttttac ttcaggaatc ggcaatacga accttccgcc
     6541 ctcacggcgt cgcggcgtcg aattcgaaat gggtgtgcaa ccagcggaga gggtcgatct
     6601 caggctcacc tatacctaca cggacgctag attcctcgaa ggtgaattcc ccggtggtcc
     6661 cttttcaatc accaacgtta atatcgtcgg caaaacggtg ccgctggtgc cgcggcacaa
     6721 ggcaaccctc aatggcaact ggggcatcac cccatcgacg aatctgtcgg gagcggtcgt
     6781 ttatgtgagc gagcaattca tggacaacga cgaggcgaac aacctcggtg tgaagattcc
     6841 cggctacacc gtggtcgatt tcaagctcac gcagcaagtc ggtgcagcgc ggtttggcgt
     6901 ggccgtgaac aatgcgctca atgaaaagta cttcaactat gcggtccgtt cacagttcac
     6961 gccggaccgc tacaacgctt atccactgcc cgaacgcacg tttatggcat tcgtggaata
     7021 caagttcgga aaataacctc agttccggga catctgtatt gactgggttc ccaagacggt
     7081 cgcctgtccg ttactgcccg gatttgttat tgtgcgggcc atgcaagaaa tgaccggcac
     7141 cgagctttat ttccgtttgc tccgttatgt caaaccatac tggggcgttt tcgcgctgtc
     7201 cattttgggt atgctgatta cggcggccac ggaagttgcg cttccggttg ccgtcaaacc
     7261 ttttctggat ggaaccttcg tcaagaagga cccgtttcta gtcacgtggg tcccggtatt
     7321 aatggtgttg ctgtatgtga ttcgaggcgc cggcgcctac ataggaacct atgccagcac
     7381 gtgggtcggc aataaagtgg tgatggattt gcgcgaccag atgttccgcc gcatgcttgc
     7441 actcccgctt ggctattttc acgacaatac aacgggcaac ttgatctcac gctttacttt
     7501 cgatgcagcc caggtaactg gtgccgcaac caatgtggtc acggtgctgg tcaaggattc
     7561 ggtcacgata ctgggactgc tgacctacct gttgtacata gactggaagc tcacgctgat
     7621 atctttgatc atggtgcccc cgattgcagt tgtggttcgc tattttaatg tccgattacg
     7681 caacatgagc cgcaagacgc aggaagcgat gggggacatt acccaagttc ttcaggaaac
     7741 catcgaatgc aataaagtcg tcaagatttt cggtggacag gcttacgaag caaaacgatt
     7801 cgcggatacg agcaacaggt tgcgcgggtt caccatgaaa cagactgctg ccgcggctgc
     7861 caatgtgccg atcgtgcaat tgctcgcagc catagccgtt gccgttgtgg tttattacgc
     7921 taccgtgcag gcacagtcgg atgccacgac agtcggcgga tttgtgtcct ttctggccgc
     7981 gatgttgctg ctgaccccgc ctctgaaaag gctcacgggc gtcgctgaat atctccagcg
     8041 cggccttgcg gccgctgaaa gcgtgttcgc gctgctggat gaaaagcccg aaacagatac
     8101 gggcaaaatt ccactgcagc gcgcccgggg cgacattgca tttcgccagg tcaatttccg
     8161 ctatgcgcgc gcagagcaca gcgcgttgag gaatgtcacg ctgtcaatta gcgcgggaga
     8221 gaccattgcg ctggttggat catcgggagg cggcaagacc acgttcgcca atatggttcc
     8281 acgtttctac catcccgact ccggtaccat tacgattgac ggacatgata tcgaagcttt
     8341 atcgctggcg gacctgcggc gcaatattgc attggtaagc caggaagtcg ctctcttcaa
     8401 cgatacagtg tcggctaaca ttgcctatgg tcaggcggcc ggagccagcc gccacgccat
     8461 tgaacgcgcc gcagaagcgg cgcatgctct ggagttcata caggcgatgc ctcacggctt
     8521 tgatacgctg atcggtgaaa atggcgttag attgtcgggt ggtcaacgcc agcggctggc
     8581 gatagcacgg accgtgctga aggatgcgcc catcctgatc ctggacgaag ccacatcggc
     8641 tctcgactcg gaatccgaga agcatgtgca ggcggcgctc gaacaattga tgcagggccg
     8701 gaccacgatc gtgatcgcac accggctgtc gactatcgaa aatgcggatc ggatagtggt
     8761 gctcgataat ggcgaaatcg cccagatagg cacccatgcc caactgctcg cggaaggtgg
     8821 aatctacgcc aaactctatc gcatccaata cgccgcgggc ggtacgcagc cagaggccga
     8881 attgcattca gttgcttgat gctggtgcgc aactgacagg gcatcgaaga ctgcggtctc
     8941 acagtggaat gttgcggtga ctattcagga aacacagtgt gtccaagcgt ccgggtccgt
     9001 caagcccgtc attgatcgga accgttgcga aggcaaacag gcgtgcgtgg cggtctgccc
     9061 ttacaacgtg ttccgcatag cgaccgtgac tgcagacttg cgccgtgggc tgagtctgcg
     9121 cgggaagttg aaggggtacg cccaccgctg gcaacaagcc tttgctgcga atgctgacgc
     9181 ctgccatgca tgcgggctat gcgttgcggc gtgtcccgag aaggcaatta cgctggttcg
     9241 ttcgatctag cggcgtccgc gtcgattacc cctgcttctc ggcccattac tccaggtcaa
     9301 caaatggcac gccgcttgcc agttcttcgg aaagcgccct atcggccgct taatcgaagg
     9361 agaacatcat gggcaaatat tttctggctt ggttactggg cgtaccgttt ttcgtattgg
     9421 tgatcctgta tctattcttt cactgagggc gttgcctcat tgatggaaat tcacggatag
     9481 catggaccag gcgcggggcc tttacgtaaa acttgcagga cagctgtaac tggtacaagc
     9541 aattttctat ctcggggagg taatttactc aagcagcctc ggcacatcgg cggcgcgcgg
     9601 tggcacaacc attgcaaacg acaagattgt agtaagtgcg tcgctaagta tagaatcgag
     9661 gagaacagac tcatgcatct tgaacacaag cctcggcccg cattccgcat cgttcgcggt
     9721 tcatcaggtc tttgggaagt tattgaagaa ggaattcgtg aagcgcttgg gttattcaga
     9781 gctccgcagg cggccttgtc ttatgcctgc catctggcgg ccatgcgcga ggggtccgtg
     9841 gtaatggtat tcgacagaat cccgatgagc acacgagcga acgtcgtcga ccatcgcggc
     9901 cctaggcgct tgcaatgatg cgtcgcgtgg agcaactacc cacaagcccc gtcaggcggg
     9961 ctgccgcggc gctggtcaaa gtcataccca tgctcgttgg ggcaacgcta ctgagtgcat
    10021 gccagcatgg ccctactgtt ttcgatgatc cgtccgcaat agctgtgctt gcggctacgc
    10081 ctggcagtgc cgtccatgga gtggtgacat tcattcgggc tggtgaggca actctggtaa
    10141 atgcgaactt atccggattt aagcccaaca gtacgcacgg catgcacata cacgtaattg
    10201 gcgactgctc tgcgcacgat gccagcagca cgggagagca tttcaacccc caatcgtccc
    10261 ggcatggtgc gccaggcgcg gccgagcatc acgccggcga cttgggaaac attactgccg
    10321 atgcagtcgg aaacgtctac gcgacgttca agatcaccga aggcagcttc ggaacgggag
    10381 atgacagcat tatcggccgc ggcctggtag tgcatgccga cagggatgac cttaattcgc
    10441 agccggctgg aaactccggc gtccgacttg catgtggcag cattacgcgc aatccagacc
    10501 gaatgaccgt tgcgaaaagc gggtaatcgc gactcttagt gtgccttgtc ccagttgggc
    10561 cccacgccga ggtcgacagc caggggaaca tccagggttg caacgctgtt cattaattgc
    10621 ggcagcccgg tcttgatcac atcaatttcc gcctccggca cttcaagcac cagttcgtcg
    10681 tgcacttgca tcacgagcct gctcttccgt ccggtagacg aaatccagcg atgcacggca
    10741 atcatggcga gcttgatcag atccgctgcg gtcccctgca tgggagcatt gattgcggca
    10801 cgctcggagc cctgacgccg tgggccattg cttgcattga tgtccggcaa gtagagcctg
    10861 cgcccgaata cggtttccac ataacccagc ttgcgggccg tttcgcgtgt ccgttccatg
    10921 tagttcctaa caccgggata gcgcaaaaaa tagcgttcca tatattgctg ggctgccgct
    10981 cgttcgatgc cgagctgcgt agcaagccca aatgccgaca tgccatagat caggccgaag
    11041 ttaatgactt tggcgtagcg acgctgctcg ctggtcacgg cggccgaatc aacaccaaaa
    11101 atttctgacg cggtgtgtct gtgcacgtcc tctccggcag cgaaggcgtg cagtaatcct
    11161 ttgtcctgtg acaggtgcgc catgatgcgc aattccactt gcgaatagtc ggctgaaaca
    11221 atgaccgagc ccggtgctgc cacgaacgct tcgcggatgc ggcggccttc cgcagtgcga
    11281 atgggaatgt tctgaagatt gggttcgtta ctggagagcc gtcccgtcac cgccactgcc
    11341 tgggcataat tggtgtgcac acggccggtc tcggcgttta ccatccgcgg cagtttgtct
    11401 gtataggtgg acttcagttt cgacaagccg cggtattcca agatcagctt cggcaaggga
    11461 tgatccagcg ccaactgctg caatacgtcc tcgtctgtcg acggtacgcc gctcggcgtt
    11521 tttttgatca ctggcagtcc ctgccgctcg aacaaaatct cctgcaattg tttcggagaa
    11581 ttcagattga acggctggcc cgcctgcagg tgcacgcttt gctcgatttc catcagcttg
    11641 ataccgagct cgcgactctg ctgttcgagc aattgcgcgt caagcagtac gccatggcgt
    11701 tccatggcga acaggacttg ggaaacgggc atctcgattt cgcgatagat gcgcaacagc
    11761 ttcgcatcct gctcgatctg cgggtgcagc acctggtgca actgcaatgc tacgtcggca
    11821 tcttccgccg cgtactcggc ggcacgagtc aattcgactt gctcaaatga aatccgcgct
    11881 gcccctttgc cggtaacatc atcgtaagtg gtcgtcgtca tgttcagatg acggcttgca
    11941 aggttatcca tgtcatgcgg acgatgactt tccagcacgt aagactgcag cgttatgtcg
    12001 tgttctaccc cggcgagttt tatcccgcag ttggcgaaga catgcctgtc gtacttcgaa
    12061 ttctgtccca gcttccgcgc tccttcgtct tccagccaat ccttcaaggc ggtcaggaca
    12121 tgttcgcggg aaagctgcat cggcgcaccg gcgtacgcat gaccgaccgg aatgtaggcg
    12181 ccgccatggg cttcaattga gagcgcgatg ccgaccagat gcgtcgtcat tggatcgagg
    12241 gtggttgttt cagtctccag cgatacgagc ggagcaggct tgattttcgc gatccacgcg
    12301 cgcaattgct catcggtaag aatggtctca taggtccgct ggtgagtgtg cggacgtgaa
    12361 acagccgttt ctttcgtatc ctgatcggcc gttcccagcg ctttatcctg aagctctcgc
    12421 aacaatcccc tgaactccag cttctcgaac agacgcctga gttcaggtac gttgtgcggc
    12481 tggggtgcca gctgctctac gtcaaccggc aacggaacgt cgcatttgat gctgagcagt
    12541 ttttttgcgg tgggcagcca tggcagcgcc ttgcgcaggt tctcgccgac tgcacccggg
    12601 atttcgtcgg catggcggat gacttcatcg agcgagccgt acaacagcag ccactttacg
    12661 gccgtctttg ggccgacttt atcgacgccc gggatgttgt ccaccgaatc gccaatcagc
    12721 gtcagatagt cgaccatctg cgccggcttg atgccaaact tctgctcgac gcccttttca
    12781 tcaagcgact cgcttgacat cgtattgacc aaactcacca tcggattgac aagctgtgta
    12841 atgtccttgt cgctggttga aatgatggtg tgcacgcccc gtgcttcagc ctgtttcgcc
    12901 aaggtgccga ttacatcgtc cgcctccacg ccatccacca tgagcagcgg ccaccccatg
    12961 gcgcggacac tatcgtgcag aggctcgatt tgtctcgcca aatcatccgg catggtcgcc
    13021 cgcgtggcct tgtattgcgg ataccaatcg tcgcggaagg tctttccttt tgcgtcgaat
    13081 acgcaggcta tataatccgc cgacgtttcg tttcttagtt tttgcagcat gctaagcacg
    13141 cctttcactg caccagtcgg ttcaccttgc cgggtgcgca agtcgggaag tgcgtgaaat
    13201 gcgcgataga ggtaggaaga tccatccacc agtactagcg ttttcattgg cgatcagcgg
    13261 gaaacatcaa catgagcgaa aaacagaagg tgccggttcc gctcgctcca gaatttgccg
    13321 agaaggcatg gtctgcgcgg gaatcatggc gagtgtttgg aattatggca gagtttgtgg
    13381 aagcgaccga gcgcctggcc catattcgcc cggcagtcag cgtattcggc agcgcgcgga
    13441 ccaggcccga tcatggctat tacgcgctcg gagaagccat cggccgcaag ctctccgacg
    13501 cggggtttgc cgtcatttcc ggcggtgggc cgggcatcat ggaatcagtc aacaagggcg
    13561 cgtacgccgg acgatcgctt tccgtaggtc tgaatatcca attgccccat gaacagagcg
    13621 ctaatgcata tcaggacgtc agccagacat tccggcattt ctttgcgcgc aaggtcatgt
    13681 tcgcaaaatt tgcgtgcgca tacgtcgtgc tacccggtgg attcggcacg ctggatgaac
    13741 tcggcgaaat cctgacgctc gttcaaactg ggaaaatccg ccgcatcccg atcattctgg
    13801 taggcggggc tttttggcgt ggcttgctgg attggatcgc agaccgcatg gtctcggaag
    13861 ccatgatcaa tccggaagat atggatttga tccagatgat tgacgatccg ggctatgtcg
    13921 tcgacgcgat attcgaccac tacgagaagc gcgggtttga gttgaccgcg gccgaacgcg
    13981 aagcgcagct caatctatag agccatttga tgtcagtttt tacgaccgtt acccgggaac
    14041 agctttccgc gtggctgaag aactactcta tcggcgttcc gacgagcctg gaaggcatcc
    14101 tgtctggaat tgaaaacact aattactttg tcaccaccac gcatggccgc ttcgtactca
    14161 cactgttcga aaagctgact cgcaatgaat tgccgttcta tctgaatctg atggcacatc
    14221 tgtccagcca cgggttgccg tgcccgaagc cgatcgcgaa tctgcaaaac cagttgcttg
    14281 gggaactcaa cggcaagcct gccgccatcg tatcttgcct ggaaggtcaa ccggtgacgg
    14341 atcccgctcc cctgcattgc gcaagggttg gcgaagttct ggcggaaatg cacctggctg
    14401 gaagaaccta ccaaggtcaa ttggataact tgcgaggcgc cgaatggtgg actgcgtgtg
    14461 cgcctgaaat ttatcctttt ctcaaacccg aagatgccga attgctcaag tccgaaatcc
    14521 actaccagca aagacagaac cggaaagggc tcccgcgtgg ggtggtgcat gccga
//
LOCUS       DATMZQ010000384         8441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1535113, whole genome shotgun sequence.
ACCESSION   DATMZQ010000384 DATMZQ010000000
VERSION     DATMZQ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535113"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2791)
                     /gene="treY"
                     /locus_tag="VN496_12435"
     CDS             complement(<1..2791)
                     /gene="treY"
                     /locus_tag="VN496_12435"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HXF17810.1"
                     /translation="MPEARHAEAYSPEHMNGERPQRARPTAETKRRQTFSPATRIPRA
                     TYRLQLHGGFRLRDAMTIIPYLAELGISHVYCSPYLRARPGSLHGYDIVDHQTLNPEI
                     GDADDLKAFVAALRAHGMAQMIDMVPNHMGVLGGDNAWWLDVLENGQASLYADYFDID
                     WRPANPDLINRVLVPVLGDHYGLVLERGEITLGFDAGTGSFSIHYYDSRFPIDPAEYP
                     HILAHVLGAASISGIPGTALGELKTVTTALSALPPRNSIAPEERPARHGAKEHCKRRL
                     AEIVAAYSEMGSAIDAALRSLNGVPGQPASFDLLHQLLEAQAYRLAFWRVAADEINYR
                     RFFDINELAALRVEDEAVFQATHKLVFELVAAGQVDGLRIDHPDGLFDPASYFQRLRE
                     TTAALAQQRPDNAGHEPPYVTIEKIIAPFETIPETWAVDGTTGYRFANLVNGLFVDTA
                     VQAPFTRIYQAFTAQRASFEEVAADCKRLVLHGALASELTMLSSLLSRIARADRHTRD
                     YTLNSLRQALTEVIIAFPVYRTYIADEVTAEDRRYIEWAVERARRRIGPAHNSIFDFI
                     KSALLAEAAGPEAAQVRYFARKFQQVTAPVMAKGVEDTSFYIYNRLLSLNDVGGDPAT
                     FGYSVSAFHGASADRAAKWPHTMLATSTHDNKRSEDVRMRIDVLSELPAAWRLKLRRW
                     SRLNRSKKRRVDDQLAPARNDEYLLYQTLIGSFPVDEGSDGLDDYCVRIERYLLKAVR
                     EAKTHTSWVNHNEEYELAVTQFVRALLSGTGRNLFLEDLREAVRPLIWLGMLNSLSMV
                     LIKFTSPGVPDCYQGNEIWDFSLVDPDNRRPVDYEVRRRLLARTDSPDEIFSHMRDGR
                     AKLHFTRRLLGLRAEVPELFMHGGYTALRVHGQQAAHVLAYARRHGRACAVAVAGRLF
                     ATLGVKENQLPCGDSI"
     gene            complement(2784..4607)
                     /gene="treZ"
                     /locus_tag="VN496_12440"
     CDS             complement(2784..4607)
                     /gene="treZ"
                     /locus_tag="VN496_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXF17811.1"
                     /translation="MKRHHHMPFGAEFDGHGAVRFKLWAPQASEVRLDIVHTQGKTNA
                     PMLPTGEGWFALTLAGIEHGTRYSFCIDQRISVPDPASRYNPQDVHGASAVVDPHAFE
                     WPDASWRGRPWEEAVIYELHVGAFTQAGTFNAAIERLNYLVRLGVTAVELMPVADFPG
                     KRNWGYDGVLPFAPDSSYGSPEDLKSLVAAAHARGLMILLDVVYNHFGPEGNYLHVYA
                     PQFFNPAHATPWGAAINFDGERSRVVRDFFIHNALYWLEEFNFDGLRLDAVHAIADDS
                     HPDIVDELAAAVRAGPGHHREVHLVLENDRNEAARLIRERGQPRYATAQWNDDVHHAF
                     HVLATGERDGYYRDYAQKPLWWLGRALAQGFGYQGEISEHRGGRARGELSAGLPPSAF
                     VNFIQNHDQIGNRALGERLSALAPDPVQRLETICLLLAPSIPLLFMGEEFSASSPFLF
                     FCDFGPELAHKVVQGRREEFAAFERFRTPQAQAGIPDPGTEQTFLASKLNWSELVKAE
                     HARWHTLISDLLELRRRYLVPHMAGSTHEARFAVDGAGLCVDWTLGDRSRLHLRANFS
                     DAAWANVAAAPGDILFAAPAGTLPGPLPGWSALWTLEPIRA"
     gene            complement(4604..6745)
                     /gene="glgX"
                     /locus_tag="VN496_12445"
     CDS             complement(4604..6745)
                     /gene="glgX"
                     /locus_tag="VN496_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXF17812.1"
                     /translation="MPARPVTAVWPGRPHPRGAHWDGEGVNFALFSEFAQKVELCLFD
                     ARGRSEIQRITLKERTDEIWHCYLPEARPGMLYGYRVHGPYQPENGHRFNPHKLLLDP
                     YAHSMDGALQWSDAHFGYTIGHKRADLSFDRRDDAAGMPKCKVIDSAFSWGEERRPDI
                     PWNDMVVYEVHVGGFTRRHPEVPPALRGTFAGLACPPVIDHLRRLGVTTIELMPVHAF
                     VDDRRLVERGLRNYWGYNTIGFFAPEKRYSASGKVAEFKTMVKTLHSAGMEVILDVVY
                     NHTAEGNECGPTLCFRGIDNAAYYRLVADDKRYYQDYTGCGNTLNMRNPRVLQLIMDS
                     LRYWVTEMHVDGFRFDLAASLARELHEVDRLGAFFDILRQDPVLSEVKLIAEPWDLGE
                     GGYQVGNFPVGWAEWNDQYRDTMRAYWKGDGGVIGDFARRLTGSSDLYGRSGRSPCAS
                     VNFITAHDGFTLHDLVSYNDKHNEANGEENRDGNSNNLSWNCGVEGPTEDAAINALRE
                     RQKRNFLATLMLSQGVPMLLGGDEMGRTQGGNNNAYCQDNEISWIDWNLDGTRRNLLE
                     FASRMIATRREHPIFRRRGFFLGLPVGDSASKDIVWLKPDGTEMSEHEWNTDFARCLG
                     VYLAGDSLGETDMRGRPVRDASFLLLFNAHHDTVPFCLPSAATSPREWFAQIDTAFNT
                     GKPQHGTLRSGEIYPLQGRSLALLRQISRVE"
     gene            complement(6793..7590)
                     /locus_tag="VN496_12450"
     CDS             complement(6793..7590)
                     /locus_tag="VN496_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXF17813.1"
                     /translation="MNQPESFHGQPSEVAGRLSLSSRHLGSEVRFASYNIHLGIGRDG
                     EFRPARIAAVINEIGADVVALQEISLGTPGFDMLRYLSDACGLTAIAGPTLVTASGQF
                     GNAMLTGLRPGEVHHWDLSMARCEPRAAIDAHLRHGSHLFRVIATHLGLRPAERRDQI
                     QRLLKIIERDRVYPTVLLGDVNEWFLWGRPLRWMHRHFQRTPAPATFPAGRPLFALDR
                     IWVEPRRALKKIAVHVSENARMASDHLPIVATLDFGPFENLRQTRTA"
     gene            complement(7737..>8441)
                     /locus_tag="VN496_12455"
     CDS             complement(7737..>8441)
                     /locus_tag="VN496_12455"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXF17814.1"
                     /translation="FAPNMAPSKSGFRWGIALMTIVVLAGLLLAWRFTPLAQLATPDN
                     IIGWAHDFAGRWWAPLLVILAYTPACFVMFPRPLITLFAIVAFGTKIGVVYAVIGVML
                     SAEMTYLAGRLFDRGTVRRVAGEKLNRVSEVLRRRGLLAVTALRLVPAGPFAVVGLVA
                     GAIRIKLWHYTVGTAIGLVPGTLATLMIGDQLEAGIQDPSTINYWPVVGAVVVLAAAA
                     FAGRRWFLNNKTVHAQ"
BASE COUNT         1724 a         2574 c         2507 g         1636 t
ORIGIN      
        1 agatcgagtc gccacaaggc agctggtttt ccttgacgcc gagcgtggcg aacagccgtc
       61 ccgcgacagc gaccgcgcat gcacgtccat ggcggcgcgc ataggcaagg acatgcgccg
      121 cttgttgacc gtgcacccgc agtgcggtgt agccgccgtg catgaacagt tcgggaacct
      181 cggcacgtaa cccaagcagt cgtctcgtga aatgcagttt ggcgcggcca tcgcgcatat
      241 ggctgaagat ctcatcggga gaatccgtgc gagccagcaa tcgccggcgc acctcgtagt
      301 cgaccggacg acgattgtcc ggatcgacca gcgaaaaatc ccagatctca ttgccctggt
      361 agcagtccgg cacccccggc gaggtgaact tgatcaggac catcgacagg ctgttcagca
      421 tgcccagcca gatcagcgga cgcaccgctt cgcgcaaatc ctccaggaac agattgcgcc
      481 cagtgccgga gagcagcgca cgcacgaact gggttaccgc cagttcgtac tcttcgttat
      541 ggttcaccca gctggtatgg gtttttgcct ctcgcactgc cttcagcagg taacgctcga
      601 tgcgcacgca atagtcgtcg agtccgtcag agccttcatc gacaggaaag ctgccaatca
      661 gcgtctggta gagcaggtat tcgtcattcc gggccggtgc aagctggtca tctaccctgc
      721 gctttttgct gcgattgagc cggctccagc gccgcaactt caggcgccac gctgccggaa
      781 gctcggacag cacatcgatg cgcatgcgca catcctcgga acgcttgttg tcatgcgtgg
      841 atgttgcaag catcgtatgc ggccatttgg ccgcgcggtc ggcgctcgcg ccgtgaaagg
      901 cactgacact gtaaccaaat gtcgctggat cgccgcccac gtcgttgagt gacagcagcc
      961 ggttgtaaat atagaaactc gtgtcttcca cgccttttgc cattacgggc gcggtcacct
     1021 gttgaaactt gcgcgcaaag tagcgcacct gcgcagcttc aggcccagcc gcttcggcca
     1081 gcagtgctga tttgatgaaa tcgaaaatgc tgttgtgagc cgggccgatg cgccggcgcg
     1141 cacgctcaac tgcccattcg atgtagcgcc ggtcctccgc ggtgacctcg tcagcaatat
     1201 aagtgcggta gaccggaaac gcgataatga cttccgtcag cgcctggcgc agggaattca
     1261 acgtgtagtc ccgggtgtgg cgatcggcgc gcgcaatgcg cgacagcaga ctgctcagca
     1321 tggtcagttc gctggccagt gccccgtgca acaccagccg cttgcagtct gctgcaacct
     1381 cttcaaacga tgcgcgctgt gcggtaaacg cctggtatat gcgcgtaaac ggcgcctgca
     1441 ccgcggtatc gacgaataac ccattaacca ggttagcgaa acgataacca gtcgtgccat
     1501 cgaccgccca cgtttccgga atcgtttcga agggcgcaat gattttttcg attgtgacat
     1561 agggaggctc atggccggcg ttgtccggcc tttgctgcgc gagcgcggca gtggtttcgc
     1621 gcaatcgctg gaaatagctc gctggatcaa acaggccatc aggatgatcg atgcgcagcc
     1681 cgtccacctg gccagccgcc acgagttcga acaccagctt gtgggttgcc tggaaaaccg
     1741 cttcgtcttc cacgcgcaag gcggcaagct cgttgatgtc gaaaaaacgc cggtaattta
     1801 tttcatcggc ggcaacgcgc cagaacgcca accggtaagc ttgtgcttcg agcaactggt
     1861 gcaggagatc gaagctggcc ggttggcccg ggacgccgtt caaggaacgc agcgctgcgt
     1921 cgattgcgct acccatctcg ctgtacgctg caacgatttc tgcaaggcgt cgtttgcagt
     1981 gttcctttgc gccgtggcgc gcaggtcttt cttccggagc gatgctattg cgcggcggca
     2041 gagcgctcag cgcagtagtc acggttttca actcgccgag cgccgtgcca gggatgccgg
     2101 aaatcgatgc ggcgcccagt acatgagcca gtatgtgcgg atactcggct ggatcaatgg
     2161 gaaaccggct atcgtagtaa tggatgctga agctgcccgt gcccgcgtcg aagccaagcg
     2221 tgatctcgcc tcgctcgagc acaagaccgt aatgatctcc gagcaccggc accagcacgc
     2281 ggttgatcag atcgggattc gccgggcgcc agtcgatgtc gaagtagtcc gcataaagcg
     2341 aagcctggcc gttttccagg acgtcgagcc accaggcatt gtccccgcca agaacaccca
     2401 tatggttggg caccatatcg atcatctgcg ccatgccgtg cgcgcgcagc gcggcaacaa
     2461 aagccttgag gtcgtccgcg tcgccgattt ccggattcag tgtctggtga tcgacaatgt
     2521 cgtagccgtg caggctgcct ggacgggcgc gaagatacgg cgaacagtaa acatggctga
     2581 tgccaagctc ggccagatac gggatgatcg tcatcgcgtc gcgcaaacga aagccgccat
     2641 gtagttgcag ccggtacgtc gcgcgcggaa tccgcgtcgc tggcgaaaag gtctgccggc
     2701 gtttcgtttc cgcggtgggc cgcgcgcgct gcggccgctc gccgttcatg tgctcgggcg
     2761 aataggcctc cgcatgtctg gcctcaggca cgaataggct ccagcgtcca taacgcagac
     2821 cagccgggaa gcggaccggg caacgttcct gctggcgcgg caaagagtat gtctcctggc
     2881 gcagctgcga catttgccca tgcggcatca gaaaaattgg cacgcaggtg cagccgggag
     2941 cgatccccta gcgtccaatc tacgcacagg cctgcgccat cgaccgcaaa gcgtgcttca
     3001 tgcgtagaac cggccatatg gggcacaagg taacggcgcc gcaactcaag cagatcgctg
     3061 atcagcgtgt gccatcgggc atgctctgct ttcacaagct cgctccaatt gagcttggac
     3121 gccagaaagg tttgttccgt tccggggtcc gggatacccg cctgggcttg cggcgtgcgg
     3181 aagcgctcga atgcagcaaa ctcctcgcgc cgtccctgca cgaccttgtg cgccaactcg
     3241 ggaccgaagt cgcagaaaaa caaaaacggc gaactggccg agaattcctc gcccatgaac
     3301 aacaatggta tcgatggggc aagcagcaga cagatcgttt ccaggcgctg caccgggtca
     3361 ggtgccaacg cgctgagccg ttctcccagc gcgcggttgc cgatctgatc atggttctga
     3421 atgaagttca cgaaggcgct tggtggcaag cccgctgaaa gttctccgcg cgcgcgcccg
     3481 ccacgatgct ctgaaatttc tccctggtag ccaaatccct gcgccagtgc gcgccccagc
     3541 caccataacg gcttctgcgc ataatcgcgg taatagccgt cgcgctcacc cgtagccaga
     3601 acgtggaatg catggtgaac atcgtcattc cattgcgcgg tcgcgtagcg cggctggcct
     3661 cgctcgcgta tcaggcgcgc ggcctcattg cggtcgtttt cgagcaccag gtgcacttct
     3721 cggtggtgtc caggccccgc gcgcacggcc gctgccagct catcaacgat atcgggatgc
     3781 gagtcgtctg cgatggcatg caccgcgtcc agacgcaagc catcgaaatt gaattcttca
     3841 agccagtaga gcgcgttgtg gatgaagaag tcgcgaacta cacgggagcg ttcgccatca
     3901 aaattaatcg ccgcgcccca cggggtggcg tgcgcaggat taaaaaactg cggtgcgtag
     3961 acgtgcaggt agttcccttc cggaccgaaa tggttataga ccacgtccag caaaatcatg
     4021 agtccacgcg catgcgccgc ggccaccagg cttttgaggt cctccggcga gccgtacgag
     4081 gaatcaggcg cgaagggcag cacgccgtcg tagccccagt tgcgcttgcc cggaaagtcc
     4141 gccactggca tgagttccac cgcagtgaca cccagccgca ccaggtaatt gagccgttca
     4201 attgccgcgt tgaacgttcc tgcttgcgtg aaggcgccga cgtgcagttc gtagataact
     4261 gcttcttccc agggacgccc gcgccaggac gcatctggcc actcaaaagc atgtggatca
     4321 actactgcgc ttgctccgtg cacgtcctgc ggattgtagc gagacgcagg atccggcacg
     4381 ctgatgcgct gatcgataca gaaggaatag cgcgtgccgt gttcgatccc ggcaagtgtc
     4441 aatgcaaacc agccttcgcc ggtcgggagc atgggcgcgt tcgttttgcc ctgtgtatga
     4501 acaatatcca gccttacctc ggaggcctgg ggcgcccaga gcttgaaacg caccgcaccg
     4561 tggccgtcga attcggcacc gaacggcatg tgatggtggc gtttcattcc actcgtgaaa
     4621 tctggcgcaa caaagcgagc gagcgaccct gcaacggata aatttcgccg ctacgcaacg
     4681 tgccatgctg tggcttgccg gtattgaagg cggtatcgat ctgcgcgaac cattccctgg
     4741 gtgaagttgc tgcgctgggg agacaaaatg gaaccgtatc gtgatgcgca ttgaacagca
     4801 gcaggaagct ggcgtcccgc accggccgcc cccgcatgtc ggtttctccc agggaatcac
     4861 cggccagata gacgccaaga cagcgtgcga aatcggtgtt ccattcgtgc tcgctcattt
     4921 ccgtcccatc cggcttgagc cagacgatgt ctttgctcgc tgaatctccg accggcagtc
     4981 cgagaaaaaa tccgcgccgc cggaatatcg ggtgttcgcg ccgcgtggcg atcatgcggg
     5041 atgcaaattc cagcagattg cgtcgcgtcc catcgaggtt ccagtcgatc cacgaaattt
     5101 cgttgtcctg acaataggca ttgttgttac cgccctgcgt gcgccccatt tcatcgccgc
     5161 ccagcaacat cggcacgccc tgggatagca tcagcgttgc cagaaaattg cgcttctggc
     5221 gttcccgcaa tgcattgatc gcggcgtcct cagtgggtcc ttccacgcca caattccacg
     5281 aaaggttatt gctgttgccg tcgcgattct cttcgccgtt ggcctcgttg tgcttgtcgt
     5341 tgtaggacac caggtcgtgc aaagtgaagc cgtcgtgagc cgtgatgaaa ttgacgctgg
     5401 cgcaaggact gcggccgctg cggccgtaca gatcgctcga tccggtcaac cggcgcgcaa
     5461 aatcaccaat cacgccaccg tcgcccttcc agtaagcgcg catggtgtcg cgatactggt
     5521 cgttccattc cgcccaaccc accggaaagt taccgacctg atagccaccc tcgccgagat
     5581 cccagggctc cgctatcagc ttgacctcag agagcaccgg atcctggcgc agaatgtcga
     5641 agaaagcccc aagccgatcg acttcgtgca actcgcgcgc cagcgatgcc gcgagatcga
     5701 aacggaatcc gtccacgtgc atttcggtga cccagtaacg cagggaatcc atgatcaact
     5761 gcagcacgcg cgggttgcgc atgttgagcg tattgccgca gccggtgtag tcctggtagt
     5821 aacgcttgtc gtccgcgacc agccgatagt aggccgcgtt gtcgatgccg cggaaacaca
     5881 acgttggtcc gcactcgtta ccctcggcgg tatggttata gacgacatcg aggattactt
     5941 ccatgcctgc gctgtgcagg gtcttgacca tggttttgaa ttcggcgacc ttgcctgatg
     6001 cgctgtagcg tttttcgggc gcaaaaaaac cgatcgtgtt gtaaccccag taattccgca
     6061 gcccacgctc aaccagccga cggtcgtcga cgaaggcatg caccggcatc agttcgatgg
     6121 tggtcacacc cagccggcgc agatgatcga tgaccggggg gcaagccagt ccagcgaagg
     6181 tgccgcgcag tgccggcggt acttcgggat gacggcgcgt aaacccaccg acgtgcacct
     6241 cgtagaccac catgtcattc cagggaatat ccggccggcg ctcctcgccc cagctgaagg
     6301 cgctgtcgat taccttgcac ttcggcatgc cggctgcgtc atcgcgccgg tcgaatgaaa
     6361 gatccgcgcg cttgtggccg atggtgtagc cgaaatgtgc atcgctccac tgcaaagcgc
     6421 catccatgct gtgtgcataa ggatccagca gcagcttgtg cgggttgaac cgatggccgt
     6481 tttccggctg atagggtccg tggacgcggt atccgtacag catgccggga cgcgcctcgg
     6541 gcaaatagca gtgccatatc tcgtcggtac gttctttcaa ggtgatgcgc tggatttcgc
     6601 tgcggccgcg cgcgtcgaac aggcaaagct cgaccttttg cgcaaattcg gaaaacaacg
     6661 cgaaattgac accttcgccg tcccaatgag cgccacgcgg atgcggacgc ccgggccata
     6721 cggcagttac cgggcgcgcc ggcatttttg aacgggtgct tcgattcgca atcgtgccgg
     6781 tattggtatc cattacgcgg tgcgggtttg tcgcaggttt tcgaaaggcc cgaagtcaag
     6841 cgttgcaacg atgggcaaat ggtccgacgc catgcgggcg ttttcactga cgtgtaccgc
     6901 aatctttttt agtgcgcggc gcggttcgac ccagatgcga tcaagcgcaa acagtggccg
     6961 gccggccgga aaagttgcag gtgccggcgt acgttggaag tgccggtgca tccagcgcag
     7021 cggcctgccc cacaggaacc attcattcac gtcacccagc agcacggtcg gataaacgcg
     7081 atccctttcg atgatcttca gcaatctctg tatttgatcg cgacgctcgg ccggacgcag
     7141 gcccaggtgc gtggcaatga cgcgaaacag atggctgccg tgcctcaagt gcgcgtcgat
     7201 tgcggccctc ggctcgcacc tggccatgct caggtcccag tggtgcactt ctccgggccg
     7261 taacccggtg agcatggcat tgccaaattg tccggatgca gtaaccaacg tcggcccggc
     7321 aatagccgta aggccgcagg catcgctcaa atagcgcaac atgtcgaagc ccggcgtgcc
     7381 gagcgaaatt tcctgtagcg caaccacgtc agcaccgatt tcgttgatca cggccgcaat
     7441 acgggcgggc ctgaactcgc catcgcgtcc aatgccaaga tgaatgttgt aggacgcaaa
     7501 cctgacctcg cttccgaggt ggcggctgct caaagacaac cgacctgcga cctcggaagg
     7561 ctgtccatgg aacgactcgg gttggttcaa cgcaaactca cgggcacgaa tgttcacgtg
     7621 gcaggcactc ggcaatcagc gatggtcgcc gagcccccgc tgttttgttt gccgtggcga
     7681 aattctgtgc gaggcttcca tggatttggc gcgagtatcc ggaagttcag attgcatcat
     7741 tgggcgtgca cggttttgtt atttaaaaac catctgcgcc ccgcaaatgc ggctgcggcg
     7801 agcacaacga cggcgccgac aacgggccag tagttgatcg tgctggggtc ctggatgcca
     7861 gcttccagct ggtccccgat catcaaggtg gcgagcgtgc ccggtaccag gccgatagcg
     7921 gtgccgaccg tgtagtgcca gagtttgatg cgtatcgcac cggcaaccag ccccaccact
     7981 gcaaaaggcc ccgccggcac caggcgcaaa gcggtgactg ccagcaggcc tctacgccgc
     8041 agcacttcac tcacgcgatt gagtttttct ccggctaccc ggcgaacggt accgcgatcg
     8101 aacagtcttc ccgcaagata agtcatctcc gcggacagca ttacgccgat caccgcatag
     8161 acgacgccaa ttttggtgcc gaaagcaaca atggcgaaca gcgtgatgag tgggcgcgga
     8221 aacattacaa agcaggccgg cgtgtaagcc aggatgacca gcagcggcgc ccaccatcgg
     8281 cccgcaaaat cgtgtgccca gccgatgatg ttgtcggggg tggcaagttg cgcaagcggc
     8341 gtgaagcgcc aagccagcag caagcccgcc agcaccacga tcgtcatcag ggctatgccc
     8401 cagcgaaatc cggatttgga cggcgccatg ttaggcgcaa a
//
LOCUS       DATMZQ010000385         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4642872, whole genome shotgun sequence.
ACCESSION   DATMZQ010000385 DATMZQ010000000
VERSION     DATMZQ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642872"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..928
                     /locus_tag="VN496_12460"
     CDS             257..928
                     /locus_tag="VN496_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17815.1"
                     /translation="MKRRFPVVILAATTMLACTNASADTSIGLSAGTLGGGVELAHAF
                     SERLGIRIGANGLNHDSTETYQSVNYDSQLKLATGELLFDWFPFANNFRISAGAMYNG
                     NKLTLTGKPSPGGTYTINGRTYSAANIGTLDGSVDFSNAAPYFGLGYGRPIGKGFSLT
                     ADLGVLFQGSPRSTLTASCGPGTPAATCTQLQSDVAAEQAQLDDDLHKYQYYPVVSIG
                     LAYTF"
     gene            complement(1000..2661)
                     /gene="ettA"
                     /locus_tag="VN496_12465"
     CDS             complement(1000..2661)
                     /gene="ettA"
                     /locus_tag="VN496_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HXF17816.1"
                     /translation="MAQYVYTMNRVGKVVPPKRIILKDISLSFFPGAKIGMLGLNGSG
                     KSSLLKIMAGEDREIEGEAIPMPNLRIGYLPQEPQLDPKQNVRQAVEGGLEEVLQAKQ
                     KLDEIYAAYAEPDADFDKLATEQAKYEAILATSGSDTEHQLEIAADALRLPPWDAKID
                     KLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLQKFPGTVVAVTHDR
                     YFLDNAAEWILELDRGRGIPWKGNYSSWLEQKEQRLEVEAKQESARIKAMQKELEWVR
                     SNPKARQAKSKARIARFEELSSHEHQKQNETQEIFIPVAERLGDSVIEFKGVSKGYGD
                     RLLIDKLSFSIPPGAIVGIIGPNGAGKSTLFRMITGKEKPDSGEIVIGSTVKLAFVDQ
                     TRDALDNKKSVWEAISNGQDILTVGKFEMPSRAYAGRFNFKGSDQQKIVGDLSGGERG
                     RLHLATTLISGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVLVISHDRWFLDRIAT
                     HILACEGESQWTFFAGNYQEYEADKKKRLGEEAAKPKRIRFKPLK"
BASE COUNT          553 a          798 c          780 g          627 t
ORIGIN      
        1 taacccaggc cattgcggta gccacaaccg taaaggtaac ggcacgcaca caactgccaa
       61 gaacgggaaa tcgttgcaag tagcgcatgg ctgtttttct ttcgatacgg gattattgtc
      121 ctccgggcgt aaagatggaa taaaggcacg gcgtttgttt gagacaattt cccgactgcg
      181 tgtaagaatg accgcacgaa cggtttttgg cgcatgccaa cgttcttacc gtctccaatt
      241 gaagacagga gttgttatga aacgacgctt tccagttgtc atccttgccg ccacaaccat
      301 gctggcttgc acgaatgcga gtgccgacac gagcatcggg ctgagcgccg gaacgcttgg
      361 cggcggcgta gaactggcac acgcttttag cgaaaggctt gggatccgca tcggagcgaa
      421 cggcctgaac catgacagca ccgaaaccta tcagtcggtc aactatgact cccaactcaa
      481 actggcaacc ggcgagttgc ttttcgactg gtttccgttt gcgaacaact ttcgcattag
      541 cgcgggagcc atgtacaacg gcaacaaact tacgctgact ggcaagccgt cgccgggagg
      601 aacctacaca atcaacggca ggacgtacag cgcggcaaac attggcacac tggatggaag
      661 tgtcgatttc agtaacgcgg caccctattt cggtctcggt tatggacggc ccatcggcaa
      721 ggggttttct ctgaccgccg atctcggcgt gctattccag ggctcgcccc gttcgaccct
      781 gactgcgagt tgcgggccgg ggacaccggc ggcgacctgt acccaacttc agagtgacgt
      841 cgcggcggag caggcacaac tcgacgatga cctgcacaag tatcagtact atccggtcgt
      901 gtcgatcggg cttgcttaca cgttctgata gttgcgcgca ggttgtatta aaaagcaggc
      961 ccgccgggcc tgcttttttt tggcagcgag gctgcgcaat tacttcagcg gtttgaaacg
     1021 aatgcgcttg ggcttggcgg cttcttcgcc gagccgcttc ttcttgtcgg cttcatattc
     1081 ctggtaattg ccggcaaaga atgtccactg cgattcgcct tcgcaggcga ggatgtgcgt
     1141 tgcgatccgg tccaggaacc agcggtcgtg cgagatcacc agcacgctgc cagcgaattc
     1201 cagcagcgca tcttccagcg cgcgcaaggt ttccacgtcc agatcgttgg acggttcgtc
     1261 cagcaataac acgttgccgc cggatatcag tgtagtcgcg agatgcaggc ggccgcgctc
     1321 gccaccggac aagtcgccga cgattttttg ctggtcggag cccttgaagt tgaagcgtcc
     1381 cgcgtaggct cgcgaaggca tctcaaactt gccgacggtc agaatgtcct gtccgtttga
     1441 gattgcctcc cagacggatt tcttgttgtc cagtgcatca cgggtctggt cgacgaaggc
     1501 aagcttcacg gtcgaaccaa tcacgatttc gccgctgtcc ggcttttcct tgcccgtgat
     1561 catgcggaac aaggtcgatt tgccggcacc gttcgggccg atgatgccaa cgatcgcacc
     1621 gggcggaata ctgaaggaga gcttgtcgat caacaggcga tcgccatacc ctttcgatac
     1681 ccctttgaac tcgatcacgc tatcgcccag ccgctccgcg accggaatga aaatttcctg
     1741 agtctcgttc tgcttctggt gctcatgcga ggacagttcc tcgaaccgcg caatgcgcgc
     1801 cttgctttta gcctggcgtg ctttcggatt tgatctcacc cattccagtt ctttttgcat
     1861 tgccttgatg cgggcagatt cctgcttggc ctcaacctcc aggcgttgct ctttctgttc
     1921 cagccaggag ctgtaattgc ccttccacgg aatgccgcgg ccgcgatcga gttcaagaat
     1981 ccattccgcc gcgttgtcga gaaaataacg gtcgtgagtc actgccacca cggtaccggg
     2041 aaatttctgc aggaactgct ccagccagtc cacgctttcc gcatcgaggt ggttggttgg
     2101 ttcatcgagc aggagcatgt ccggtttcga aagcagcaaa cggcacagcg cgacgcggcg
     2161 tttctctccg cctgagagct tgtcgatctt ggcatcccat ggcggcaggc gcagcgcatc
     2221 ggctgcgatc tcgagctgat gttccgtatc gctaccactg gtagcaagga tcgcttcgta
     2281 cttggcctgc tcggtggcga gcttgtcgaa gtcggcatcc ggctccgcat acgcggcata
     2341 aatttcgtcc agcttctgct tcgcttgcag cacttcttcc aaaccacctt caacggcctg
     2401 gcgtacgttc tgcttcggat cgagctgcgg ctcctggggc aggtagccaa tgcgcagatt
     2461 cggcatcgga atcgcttcgc cttcgatctc cctgtcctca cccgccatga ttttcagcag
     2521 gctggatttg cccgagccat tcagtccgag catgccgatc ttggcgccag ggaaaaacga
     2581 gagggaaata tccttgagga tgatacgttt gggcgggacg accttgccca cgcgattcat
     2641 cgtgtaaacg tattgagcca tgggtcgaat gaagcctgtg gtcgttcggg ccgcaattat
     2701 cgcaggctga gcggggagcc gccggcgccc ggtcagaatc gggcaagggt caagcccg
//
LOCUS       DATMZQ010000386         4336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4642900, whole genome shotgun sequence.
ACCESSION   DATMZQ010000386 DATMZQ010000000
VERSION     DATMZQ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642900"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VN496_12470"
     CDS             <1..493
                     /locus_tag="VN496_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXF17817.1"
                     /translation="GADFGRSFSLTDHTGKQRTLQDFRGKVVVVFFGFTHCPDVCPTT
                     LAELANAIRKLGPAGEQVQVLMVTVDPARDTPELLAQYVTAFDPRFLALRGTTDETSR
                     VAKEFKVIFQKVAGPAPQNYSMDHSAGSYIFDRQGRLRLYVAYGRGADVFAHDIDLLL
                     KQP"
     gene            complement(542..1402)
                     /gene="rpoH"
                     /locus_tag="VN496_12475"
     CDS             complement(542..1402)
                     /gene="rpoH"
                     /locus_tag="VN496_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HXF17818.1"
                     /translation="MTNAMALSIPFPSTAGSLDTYIHAVNRFPLLSQEEETALARRLR
                     DEDDVESARRLVLSHLRVVVSIARGYLGYGLPHADLIQEGNVGLMKAVKRFDPDRGVR
                     LVSFAVHWIRAEIHEYILRNWRMVKVATTKAQRKLFFNLRSLKRSHATMTPDEVDAIA
                     QQLNVKSGDVVEMETRMGGHDIALEPLSDDEEENFSPIAYLTDATAEPAAALEHEQSE
                     RARSKGLETALTSLDARSRRIIEARWLREEDGATLHDLAGEFGVSAERIRQIEGQAMK
                     KIRGMMVSAE"
     gene            complement(1494..2399)
                     /gene="ftsX"
                     /locus_tag="VN496_12480"
     CDS             complement(1494..2399)
                     /gene="ftsX"
                     /locus_tag="VN496_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXF17819.1"
                     /translation="MNWLLVHLDTLRESLARLVRHPIASALNIIVIGIALSMPAGFYL
                     GLSNLQTFSRQLSSDPQVSIFMNVDASPVEVAAVEQRLKANQDVGRVVFITRDQALVH
                     LKRSAGLSDVLANLGRNPLPDAFVVTARSNDPATLEALHDEARTWPKVEHVQLDAAWA
                     RRLDAALNIGRMLVTLLAVLLAVALVAVTFNTIRLQILTRRDEIEVSKLIGATHAFIR
                     RPFLYFGAMQGLAGGFAAWLIVAVAVLVLNVQLADLAGLYGSMFRLEWPDAGYTMGLL
                     GFSAALGWLGAWLSVSRHLWQMRPL"
     gene            complement(2396..3049)
                     /gene="ftsE"
                     /locus_tag="VN496_12485"
     CDS             complement(2396..3049)
                     /gene="ftsE"
                     /locus_tag="VN496_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXF17820.1"
                     /translation="MIKVSHVAKRYPGGYEALRDVSFSVDAGEMVFITGHSGAGKSTL
                     LKLITAIERPTAGSVFINNQNISAMKRGMIPFFRRNFGLIFQDHKLLFDRSVFENVAL
                     PLSITGFPHKEIAKRTRAALDKVGLLGREKARPITLSGGEQQRLCIARAIVNRPAILL
                     ADEPTGNLDSAYAGEIIGMFKSFNQVGVTVVIATHDSSMIAQLGPRVLRLEHGRIIA"
     gene            complement(3073..4035)
                     /gene="ftsY"
                     /locus_tag="VN496_12490"
     CDS             complement(3073..4035)
                     /gene="ftsY"
                     /locus_tag="VN496_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXF17821.1"
                     /translation="MLSFFKRDKVDNTQSDSGTGWTAKLRQGLAATRARLSAQLVGVF
                     GAGRAIDETLFEEMESVLLSCDVGVEATTYLLDTARAKARSQGLSDASQIREVLKQAL
                     LELLTPLEQPLDISHAKPFVILIAGVNGAGKTTSIGKLARFFQMHGQSVVLAAGDTFR
                     AAAREQLKVWGERNDVTVIAQEGGDTAAVIYDAIQSAKAKNTDIVIADTAGRLPTQLH
                     LMDEIRKVKRVIAKAIPDAPHEILLVLDANNGQNAVAQVKAFDAALGVTGLILTKLDG
                     TAKGGAIAAIARHKPIALRFIGVGEAIDDLRPFSAQQFVDALFE"
     gene            4138..>4336
                     /locus_tag="VN496_12495"
     CDS             4138..>4336
                     /locus_tag="VN496_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXF17822.1"
                     /translation="MLTDNLAVRSALAALMLVCVSISVRAETVAQRTLPNGLNMLVKE
                     DHRSPVIVTMVWYRAGSMDEVS"
BASE COUNT          939 a         1259 c         1166 g          972 t
ORIGIN      
        1 gggtgcagat ttcggcagat ctttttcgct gaccgaccac accggcaaac aacgcacgtt
       61 gcaggatttc cgcggcaaag tggtagttgt attttttggc tttactcact gcccggatgt
      121 ttgtccgacg acgttagccg agttggcgaa tgccatcagg aagcttggtc ccgctggaga
      181 acaggtacaa gtgctgatgg tcactgtcga tcctgcgcgc gacacgccgg agctgctagc
      241 ccaatatgtg acggcgtttg atccaagatt cctcgccttg cgagggacaa ccgacgagac
      301 atcgcgagtc gccaaggaat tcaaagtcat cttccagaaa gttgccggtc cggcgccgca
      361 aaactattcg atggaccact cggccggcag ctacattttc gaccgtcagg ggcgcttgcg
      421 gttgtatgtg gcctacggtc gtggagcgga tgtctttgcg cacgacatcg atttgttgct
      481 caagcaacct tgataaaaag aaaggcccca taaggggcct tctctacaga tttgcaatac
      541 cttactcggc agaaaccatc atgccgcgta tctttttcat ggcctgaccc tcgatctggc
      601 ggatgcgttc tgctgagacg ccgaattcac cggccaagtc gtgtagtgtt gccccgtctt
      661 cttcgcgcaa ccaacgcgcc tcgatgatcc tgcggctgcg cgcgtcaagc gatgtaagcg
      721 cagtttccag acccttgctg cgagcgcgct ccgactgctc gtgttccagc gccgcagccg
      781 gctcagccgt tgcatcggtc agataagcga tcggcgaaaa gttctcttcc tcatcatcgc
      841 tcagtggttc cagagcgata tcatgcccgc ccatccgggt ttccatttcg actacatcgc
      901 cggacttgac gttcagttgc tgagcaattg catcaacctc gtctggcgtc atggtggcat
      961 ggcttcgctt caggctccgg agattgaaga atagcttgcg ttgtgccttg gtcgtggcca
     1021 cctttaccat gcgccagttc ctcaggatat attcgtgtat ttcagcgcga atccaatgca
     1081 ccgcgaatga caccagacgc acaccgcgat cggggtcaaa gcgctttacc gctttcatca
     1141 gcccgacgtt accctcctgg atcaggtcgg catgcggtaa tccataaccc agatagccgc
     1201 gagcaatcga cacgacaacg cgcaagtgag acagcacgag ccggcgtgcg gattccacat
     1261 cgtcttcatc gcgaagccga cgcgccaatg ccgtttcctc ttcctggctt agcagcggaa
     1321 agcgatttac ggcatggata taagtatcca ggctgcccgc cgttgagggg aacgggatcg
     1381 aaagcgccat agcattagtc atcagacaaa tcctccttta ccaaatatta gcactcgcaa
     1441 cgtaagagtg ccaatcacct cataagttcg cgtacgctgg cacagaagcc gcgctacagt
     1501 ggccgcattt gccataagtg gcgactcact gatagccagg cgccaagcca tcccagggcg
     1561 gctgaaaatc ccaacaatcc catggtgtaa ccagcgtccg gccactccag ccggaacatg
     1621 gaaccataga gtcccgccag gtcagcgagt tgcacgttta gcaccagcac cgcaacggcc
     1681 acgatcagcc atgcggcgaa gccaccggcc aagccttgca tagcgccgaa atacaggaac
     1741 ggccgccgaa tgaaggcgtg ggttgcgcca atcagtttgg agacctcgat ttcatcgcgg
     1801 cgcgtgagga tctgtaaccg tatcgtattg aacgtcaccg caaccagggc tacagccagt
     1861 aataccgcca gcaacgtgac cagcatacga ccgatgttga gggcggcatc cagtcttcgt
     1921 gcccaagccg catccagttg cacatgttcg accttcggcc aggttctcgc ctcatcatgc
     1981 agcgcttcca gcgtggcggg gtcgttgctg cgggcggtga caacaaatgc atccggcaat
     2041 ggattgcgtc ccagattagc caggacgtcc gagagccccg cgctgcgctt gaggtggaca
     2101 agggcctggt ctcgcgttat gaaaacaaca cgccctacgt cctgattggc tttcaatcgc
     2161 tgttccaccg cggcgacctc taccgggcta gcgtcgacat tcatgaagat actgacttgc
     2221 ggatcactgg agagttgcct ggaaaatgtc tgcagattgc tcaatccaag atagaacccc
     2281 gcgggcatgg acaaggcgat cccgattacg atgatattca gcgctgatgc gatcggatgg
     2341 cgtaccaatc gtgccaggct ttctcttaac gtatcgagat ggaccagaag ccagttcatg
     2401 caatgatccg gccgtgctcc agacgaagca cacgaggccc gagctgcgcg atcatgctgg
     2461 aatcatgcgt tgcgatcacg acggtgacac cgacctgatt gaacgacttg aacatgccaa
     2521 taatttctcc ggcgtaggcc gaatccaggt ttcccgtcgg ttcatcggcg agcaaaatgg
     2581 ctggacgatt gactatggcg cgggcaatgc acagtcgctg ttgctctcct ccggacaaag
     2641 tgattggcct ggctttctcg cgtcccagca ggccgacctt gtccagtgcc gcccgcgtgc
     2701 gtttcgctat ttccttgtgc ggaaagccgg taatcgaaag gggcagtgcc acgttttcaa
     2761 aaacgctgcg atcaaacagc agtttgtgat cctgaaaaat caatccgaaa tttcgccgga
     2821 aaaacggaat catgccccgc ttcatagcgg aaatgttctg gttattgata aaaacgctgc
     2881 cggcggtagg ccgttcgatc gcggttatca gcttaagaag ggtacttttc ccggctcctg
     2941 aatgtccggt aataaaaacc atctcgcccg catccacgga aaaacttaca tcgcgcaaag
     3001 cctcgtaacc gccgggatag cgcttcgcga cgtggctcac tttgatcatc gtgcccttga
     3061 tttgcgaggc actcactcga acaacgcgtc cacaaattgt tgtgcggaaa acggtcgcag
     3121 atcgtcgatg gcctccccca cgccgataaa acgcaacgca atgggcttat ggcgcgctat
     3181 ggcggcgatg gcaccgccct tagccgtgcc gtccaatttg gtcaatatga gtcccgtcac
     3241 ccctagcgcg gcgtcgaatg cctttacttg agccactgcg ttctgaccgt tgtttgcgtc
     3301 gaggaccagc aatatttcgt gcggcgcatc cggaattgcc ttggcaatga cacgttttac
     3361 tttgcgaatt tcgtccatca ggtgcaattg cgtaggcagg cggcctgcgg tatcggcaat
     3421 cacaatatcc gtgttcttcg ccttggccga ctggattgcg tcgtatatca ccgccgctgt
     3481 atcgccgcct tcctgagcta tgacggttac gtcattgcgc tcaccccaca ccttcaattg
     3541 ctctcgtgca gcggctcgga aggtatctcc ggctgccagc acgacggact gaccgtgcat
     3601 ctgaaaaaag cgagctagtt tgccgatgga agtggttttg cccgctccat tgacaccggc
     3661 gatcaggata acaaagggct tcgcatgcga tatatccagc ggctgttcca gcggtgtcaa
     3721 tagttcgagc aacgcctgtt tgagtacttc gcgaatttgc gaagcgtcgc tcaacccttg
     3781 ggagcgcgcc ttggcccgtg cggtgtcgag aaggtaggtg gtcgcttcca ccccaacgtc
     3841 acaggacagc aggacagact ccatttcttc gaacagcgtt tcatcgatcg cgcgccctgc
     3901 gccgaacacg ccgaccagct gtgcagacag gcgcgctcga gtggcagcca gtccttgccg
     3961 cagcttggcg gtccagccgg tgccagagtc gctctgggtg ttatcgacct tgtcacgctt
     4021 gaagaaactt aacatactgt cccgctgtca tagccgacgc gtttgggtct ttgcatttag
     4081 tcaattctat tctgattttc ttcacttcca ttctacaaat ttccggttct acaaagcatg
     4141 ttgaccgata acttggcggt tcgatccgct cttgcagcat tgatgttggt ttgcgtcagc
     4201 atcagcgtgc gcgctgaaac agtcgcgcag cgaacgttgc ccaatggact caacatgctg
     4261 gtgaaggaag accaccgttc tccggtcatt gttaccatgg tgtggtatcg cgcggggagc
     4321 atggatgaag tcagca
//
LOCUS       DATMZQ010000387         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1536500, whole genome shotgun sequence.
ACCESSION   DATMZQ010000387 DATMZQ010000000
VERSION     DATMZQ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536500"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VN496_12500"
     CDS             <1..568
                     /locus_tag="VN496_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HXF17823.1"
                     /translation="GDGALTFFDWVKERSHLFRGVTVGTALNDGAFHFNRLGTFLERA
                     DNTARILDVKYHVLLPKVEDVGGAVDYYQWAAVLRSVSAFESYRKVYRDVITPLRVAE
                     LLILRRDMPRSLHACMEESYEIFQTITTPYSGEALRRAGELHAGLRFGRIDVIFSSGL
                     HEYLIEFLNRLQELGDEVNRSFFNVAYA"
     gene            625..1359
                     /locus_tag="VN496_12505"
     CDS             625..1359
                     /locus_tag="VN496_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HXF17824.1"
                     /translation="MTYCVAIKLDSGMLFASDSRTNAGVDHIATFSKMRIFEQKDERM
                     FVILSSGNLAVTQGVMNILDRHKHFEPDRQSIWNVASMFDVATLVGDALRDIQRRDGP
                     YLAQNNIDFSATLLLGGQVRGEEPRLFNIYAQGNFIEALSDTIYFQVGESKYGKPILD
                     RVLSSATPYREAAKCALISFDSTIRSNISVGLPIDLAWYPRDALRMGVQKRIGEGDAY
                     FSMIRQSWGSGMRQLFSELPNPDWLS"
     gene            1385..>2575
                     /locus_tag="VN496_12510"
     CDS             1385..>2575
                     /locus_tag="VN496_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXF17825.1"
                     /translation="MSIRVALTHETTYHFDRPVALSPHEIRLRPAPHCRTPINSYSLS
                     VTPKHFINWQQDPFGNWLARLVFPAKATELKVKVDLVADMTVINPFDFFVESYAEAFP
                     FEYPQDLLKELQPFLETEAAGALMSGWLAAFRREVLTGKLAIVDMLVAANSRLQRDIG
                     YIVRMEPGIQPCEETLALRRGSCRDSAWLLVQIFRHLGLAARFASGYLIQLTADVKAL
                     DGPSGPDKDFTDLHAWTEVYIPGAGWVGLDPTSGLFAGEGHIPLACTPAPSSAAPVIG
                     YTDVSEVEFGFHMSVSRVHEDPRVTLPYSEQRWADIDALARKVDSQLAAWDVRLTMGG
                     EPTFVSIDDMDGAEWNTEAQGERKRELAGELWLRLHERFCPGGLLHEGQGKWYPGEPL
                     PRWAL"
BASE COUNT          494 a          748 c          786 g          547 t
ORIGIN      
        1 cggcgatggc gccttgacgt ttttcgactg ggtcaaggaa cgatcgcatc tgtttcgcgg
       61 cgtaaccgtt ggcactgcgc tgaatgatgg cgcgtttcac ttcaaccggc tcggaacgtt
      121 tctggagcgc gccgacaata cggcccgcat tctggatgtg aagtatcacg tgctgctgcc
      181 caaggtggag gatgtcggcg gcgccgtgga ttattaccaa tgggcggcag tgctgcgttc
      241 ggtgtcggcc ttcgaatctt atcgcaaggt ctaccgcgac gtgatcacgc cgctgcgtgt
      301 tgccgagctg ctgatcctgc gacgcgacat gccgcgttcg ctgcatgcgt gcatggaaga
      361 gtcttatgag atcttccaga ccatcacgac gccttattcg ggtgaggcgc tgcgccgcgc
      421 cggcgaactg catgccggat tgcgcttcgg ccgtatcgat gtcattttca gcagcggctt
      481 gcacgagtat ctgatcgaat tcctgaaccg gctgcaggag ctcggcgacg aggtaaaccg
      541 cagcttcttc aatgtcgcct acgcctgaac cggacgcgtt ttgcggctgc gttatgatgc
      601 gcttcccgcc ttgatggaac cttcatgact tactgcgtcg caatcaagct cgattcgggc
      661 atgttgttcg cctcggattc acgcaccaac gccggcgtgg accacatcgc caccttcagc
      721 aagatgagaa ttttcgagca gaaggacgag cgcatgttcg tcattctgtc ttccggaaac
      781 ctggcggtca cgcaaggagt gatgaacata ctggaccggc acaaacactt cgagccggac
      841 cggcagtcca tctggaacgt cgccagcatg tttgacgttg ccaccctggt tggggatgca
      901 ctgcgtgaca tccagcgccg cgatgggccg tatcttgcgc agaacaatat tgatttcagc
      961 gcgacattgc tgctcggtgg ccaggtcagg ggcgaggagc cgcgcctgtt caacatttac
     1021 gcccaaggca actttatcga ggcgttgtcc gatacgatct acttccaggt gggcgagagt
     1081 aaatacggca aacctattct ggaccgtgta ttgagcagcg cgacgccgta ccgtgaagcc
     1141 gccaaatgcg cgttgatctc gtttgattcc accattcgca gcaacatctc ggtgggcttg
     1201 ccaatcgatc tggcctggta tccgcgcgac gcgctgcgca tgggggtgca gaagcgcatc
     1261 ggtgaagggg acgcgtattt ttccatgatt cgccaaagct ggggcagtgg catgcggcag
     1321 ctgttcagcg aattgccaaa cccggattgg ctttcatgac tgaccgcggg gcgagcttag
     1381 tctcatgagc attcgcgtcg cgctcacgca cgagacgact taccactttg accggcccgt
     1441 cgcactgtca ccccatgaga tccgcctgcg cccggcgccc cattgccgca cgccaatcaa
     1501 cagctattcg ttgagcgtaa cgccgaagca tttcatcaac tggcagcagg atccattcgg
     1561 caattggctg gcgcggctgg tgttcccggc caaggccaca gaactgaagg tcaaagtcga
     1621 cctggtggcc gacatgaccg tcatcaatcc tttcgacttt tttgtcgaaa gttacgccga
     1681 agcgtttccg ttcgagtacc cgcaggattt gctgaaagag ctgcagccat ttctggaaac
     1741 agaagcagcc ggcgcactga tgtccggatg gctggcggct tttcgccgcg aggtgctgac
     1801 cggaaaactc gccatcgttg atatgctggt tgcggccaac tcacgtctgc agcgcgatat
     1861 tggctatatc gtgcgcatgg aaccgggcat ccagccctgc gaagaaacgc tggcgctgcg
     1921 ccgtggctcc tgccgcgatt cggcctggct gctggtccaa atctttcgcc acttaggact
     1981 tgcggcccgc ttcgcatccg gctatctcat ccagctcacg gcggacgtca aagcgcttga
     2041 tgggccgagc ggacccgaca aggacttcac cgacctgcat gcgtggacgg aagtgtacat
     2101 acccggtgcg ggttgggtcg ggctcgatcc gacgtcaggc ttgtttgccg gcgaaggaca
     2161 catcccgctt gcatgcacgc ccgcgccctc gagtgccgcg cctgtcattg gctacaccga
     2221 tgtcagtgaa gtggaattcg gatttcacat gagcgtgagc cgcgtgcacg aagatccgcg
     2281 cgtcacattg ccgtatagcg agcagcgttg ggccgacatc gatgcgcttg cgcgcaaggt
     2341 tgacagccag cttgctgcgt gggacgtgcg cctgacaatg ggaggagaac cgaccttcgt
     2401 gtcgatcgat gacatggacg gcgcggagtg gaacaccgaa gcgcaaggcg agcgcaagcg
     2461 cgaactggcg ggtgaattgt ggctgcgtct gcacgaacga ttttgcccgg gtggcctgct
     2521 gcatgaaggc cagggcaagt ggtatccggg cgaaccgctg ccgcgctggg cgctc
//
LOCUS       DATMZQ010000388         2814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4643242, whole genome shotgun sequence.
ACCESSION   DATMZQ010000388 DATMZQ010000000
VERSION     DATMZQ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4643242"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VN496_12515"
     CDS             <1..181
                     /locus_tag="VN496_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17826.1"
                     /translation="WKLTGAGHVWPGGAGDYLARMLGSQKILGPPTDVIDANTRIWQF
                     VSPYHSSFVRQQIPD"
     gene            complement(190..909)
                     /locus_tag="VN496_12520"
     CDS             complement(190..909)
                     /locus_tag="VN496_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17827.1"
                     /translation="MSEQFAGQTVLVAGGTGGLGRAVSLMFLQQGARVAVTYQKQDEW
                     DDLEEAAKHHAKRLERHMVDVTNESAVSQLVATIVAQRGALDVLVNTVGGYAGGAPVW
                     ETQSDVLDHMLTLNLRSGFSLARAVIPAMLKRQHGAIVNVAAKAAIEHPAGAGAYAAS
                     KAAALALLDSLAADVRGKGVRVNTILPSIIDTPANRSAMPKADFAKWPKPEEIARVVL
                     FLCSEDARVIHGAAIPVYGDS"
     gene            complement(902..1855)
                     /locus_tag="VN496_12525"
     CDS             complement(902..1855)
                     /locus_tag="VN496_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HXF17828.1"
                     /translation="MQSHNKQQPMASGRVTDDTWPALPLDAWRDTYATLHMWTQVVGK
                     ICLALTPRSNHFWNVAFHLTSCGLATPVLTVGERALTLTFDFVSHRLILQCSDSRSES
                     IPLEPRTVAEFYRMVIDTLHRMGVHVRIWTVPVEIPDPIRFEADNVHRSYDRAQANAF
                     WRALVAMKPVFEGFRCGFVGKCSPVHFFWGSFDLAVTRFSGRRAPERKGADAITREAY
                     SHEVISHGFWPGSGAVQQPAFYAYAAPEPTGLREAVVRPAAAFYSKELSEFILPYEAV
                     RSAQSPAADLAAFLESTYERAATLARWNRAELERSTEPAGE"
     gene            complement(1878..>2814)
                     /locus_tag="VN496_12530"
     CDS             complement(1878..>2814)
                     /locus_tag="VN496_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF17829.1"
                     /translation="IELVLNARVTSIDVQKKRVVLGDGRSYQFDAMLIATGADPVRLP
                     VPGATDAQIYYLRTFADSRAIIARAMSATRVVVAGASFIGLEVAASLRARGIEVHIVA
                     PDRTPLERVMGSQVGTFVRELHEQHGVVFHLGQTISRVDGRKVSLSDASVLDADFLVL
                     GVGVRPSTTLAEQAGLALDRGVVVDEYLQTSAPGVFAAGDIARWPDPHSGERIRVEHW
                     VVAERQGQVAARNILGRRERFDAVPFFWSQHYDVSINYVGHAENWDAVEIDGSLQAKD
                     CAVTYKRGKRALAVATIFRDAQSLKAEVAMERNGK"
BASE COUNT          562 a          907 c          800 g          545 t
ORIGIN      
        1 gtggaagttg accggcgccg gccatgtctg gcctggcggc gcgggcgatt acctggccag
       61 aatgctcggc tcgcagaaga ttctcggccc tcctaccgat gtcatagatg ccaatacgcg
      121 gatatggcaa ttcgtctcgc cctaccatag cagcttcgtc cggcagcaaa tacccgactg
      181 acctgtggct tagctgtcgc cgtataccgg gatggccgcc ccgtgaatga cgcgcgcgtc
      241 ctcgctgcac aggaacagaa ccacgcgcgc gatctcctcc ggtttcggcc acttcgcgaa
      301 gtccgccttc ggcattgcac tgcggttggc tggcgtatcg atgatgcttg gcagaatcgt
      361 gttgacccgc acgcctttgc cccggacatc ggcagccagt gaatcgagca acgccaacgc
      421 ggcagccttc gaagcggcgt atgcgccggc accggcggga tgttcaatcg ccgccttggc
      481 cgccacgttg acgattgctc cgtgttggcg tttgagcatt gcaggaatca cggcgcgtgc
      541 cagcgagaaa ccggaacgca ggtttaacgt gagcatgtga tccagaacat cgctttgggt
      601 ttcccagact ggtgcgccac cggcgtaccc gccgacggta ttgaccaata catccagcgc
      661 gccgcgttgc gcgacaatcg tagcgaccag ctggctaact gctgattcat tcgtcacatc
      721 gaccatgtgc ctttcaagcc gtttcgcgtg atgcttggct gcctcttcca aatcatccca
      781 ctcgtcctgc ttttggtaag ttacggcaac ccgggcaccc tgctgcaaga acatcaggct
      841 gaccgcgcgg cccaaaccgc cggtgccacc ggcaaccagc acggtctgtc ctgcgaattg
      901 ttcactcacc tgccggctcc gtacttcgtt ccagctcggc gcgattccat cgtgcgagtg
      961 ttgctgcgcg ctcataggta gattccagaa atgccgctaa atcggccgcg ggggattgcg
     1021 cgctgcgcac ggcttcgtat ggcaggatga actcagacaa ttccttgctg tagaaggccg
     1081 ccgccggacg caccacggct tccctgagcc cggtgggctc cggcgccgca tacgcgtaga
     1141 acgcgggttg ctgaaccgcc ccgcttccgg gccagaagcc gtgactgatg acttcatggg
     1201 agtaggcctc gcgcgtaatc gcgtctgcgc ctttgcgctc cggcgcacgg cgaccggaga
     1261 aacgcgtcac agcaagatca aaactacccc aaaagaaatg caccgggctg cattttccaa
     1321 caaagccaca gcggaaacct tcgaacaccg gcttcatcgc caccagcgcg cgccagaatg
     1381 cgttcgcctg cgcgcgatca taagaccgat gaacattatc cgcttcgaag cggatcggat
     1441 cgggtatttc aaccggaacg gtccagatgc gaacgtgcac gcccatccgg tgcagcgtat
     1501 cgatcaccat gcgataaaac tcggctacgg tacgtggctc cagtggaatg gactcactgc
     1561 gactgtcgga gcattgcagg atcagtcgat gtgaaacgaa gtcgaaggtc agcgtcaagg
     1621 cacgctcgcc gacggtcagc accggtgtcg caaggccgca agaagtcagg tggaacgcca
     1681 cattccagaa gtgattggag cgtggcgtca acgcgaggca gattttgccc acgacctgtg
     1741 tccacatatg caacgtcgca taagtatcgc gccaggcgtc tagcggcagt gccggccagg
     1801 tatcgtccgt cacacggccg ctggccatcg gttgctgctt gttgtggctt tgcatatcga
     1861 acccgattcc ccaacaccta cttgccgttt cgctccatgg ccacttccgc tttcaggctt
     1921 tgcgcgtcgc gaaatatcgt cgccacggca agcgcgcgct tgccgcgctt atacgtgacc
     1981 gcgcagtcct tcgcctgcag tgaaccgtct atttcgacgg catcccaatt ttctgcatgg
     2041 ccgacataat taattgaaac gtcgtaatgc tggctccaga agaatggcac agcgtcgaaa
     2101 cgctcacgcc ggcccaggat gtttctggcc gcgacttgtc cctgacgttc cgccaccacc
     2161 caatgttcga cgcgaatgcg ttcgcctgaa tgcggatccg gccagcgcgc aatgtcgccg
     2221 gccgcgaata cgccgggcgc gctggtctgc agatattcat cgaccacgac gccgcgatcc
     2281 agggccaggc cggcctgctc ggccagcgtg gtcgaaggtc ggacgcccac gccaaggacg
     2341 aggaaatccg catccagcac gctcgcatcg ctcaacgata cttttcggcc atccactcgg
     2401 ctgatagtct gccccagatg aaacaccacg ccatgctgct cgtgcagttc gcgcacaaaa
     2461 gtgccgactt gcgatcccat tacgcgctcc aatggcgtcc ggtcgggcgc gacgatatgc
     2521 acttcaatgc cacgagcgcg cagcgaggcg gccacctcaa ggccgatgaa acttgccccc
     2581 gcgacgacaa ccctggtcgc cgacatcgcg cgggcgataa tggctcgcga atcggcgaac
     2641 gtccgtaaat aatatatttg cgcatcggtt gcgccgggaa ccggcaggcg caccgggtcc
     2701 gcaccggtag cgatcagcat tgcgtcaaac tggtagctgc gcccgtcgcc cagtacaacg
     2761 cgttttttct ggacatcgat cgatgtcaca cgcgcgttca gtaccagttc gata
//
LOCUS       DATMZQ010000389         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_652624, whole genome shotgun sequence.
ACCESSION   DATMZQ010000389 DATMZQ010000000
VERSION     DATMZQ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652624"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VN496_12535"
     CDS             complement(<1..318)
                     /locus_tag="VN496_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXF17830.1"
                     /translation="MQVSSNRFKQAASEQLANQGVQKALRNAKGKLVVARSRSILELD
                     NFEEIREAAARIRDFGLQHMDVLLEEWEKRAEAAGTIVHWAESPQQVNQLVLEIARKH
                     AVRK"
     gene            complement(334..1092)
                     /locus_tag="VN496_12540"
     CDS             complement(334..1092)
                     /locus_tag="VN496_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXF17831.1"
                     /translation="MKVGLFVTCLVDLMRPSIGFASLRLLELAGYEVVVPAGQTCCGQ
                     PGYNSGDRAAAQALAKKLIDEFAGCEYVVVPSGSCAGMIRTHYPELLTEMPELNAAVE
                     SLARKTYELTDFLVNVARIASVPGQFKGSVTYHDSCSGLREMNVKEQPRALLSKVNGL
                     SLREMQQPEQCCGFGGTFAVKYGEISTRIADLKCADVQSVAADALVLGDLGCMLNIEG
                     RLRRRGDQDTRVWHVAEVLAGMADDVDTNSHARA"
     gene            1261..2040
                     /locus_tag="VN496_12545"
     CDS             1261..2040
                     /locus_tag="VN496_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXF17832.1"
                     /translation="MAEPNGKTSIQVIERMMDLLDALARNEEPVNLKRLANATSLHPS
                     TAHRILNMMVEFRMVDRIEPGTYRLGIRLLELGNLVKSRINLRKEALPFMRLLHQELH
                     ETVNLSVRQGDEMVYVERLLSDRTAMRVVHLIGARAPLHVTAAGKIFLLEDGAEKCRE
                     YAQRTGLEIFTKNTIRDLAALAKELERVRKLGYALDNEEAENGVSCIGAGIYDDEGRL
                     VAGLSLSAPSKRMDKGWCSKVKHVADEISRALGHLPGAKAA"
     gene            complement(2067..>2835)
                     /locus_tag="VN496_12550"
     CDS             complement(2067..>2835)
                     /locus_tag="VN496_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXF17833.1"
                     /translation="AALRLRSGAVLVLAQDTGLPLFAKNVDQIMPIASITKLMTAMVL
                     LDSGLPLLESIDIDKSDVDRRKGSRSKLKVGTSLMRAEMLKLALMASENRAAAALARN
                     FPGGTEAFVAAMNQKAVEIGMVNTHFEDPTGLTPDNVSTAEDLAKMVAAAHGYPLIRE
                     YTTSDSFKIDSEDRRRHRLLSYVNSNRLVKSRQWDIGLSKTGYISEAGRCLVMQAMIA
                     DRPVIIVLLDSWGKLSRIADANRIKRWIEADLLRISG"
BASE COUNT          598 a          826 c          777 g          634 t
ORIGIN      
        1 ttttcgcacg gcgtgcttgc gggcaatttc aagcaccaat tggttaacct gctgtggcga
       61 ttcggcccag tgcacgattg ttccggctgc ttcggccctt ttttcccact cttccagcaa
      121 cacatccatg tgctggaggc cgaagtcccg aatacgcgcc gctgcttcgc ggatctcttc
      181 aaaattgtcc agctcaagaa tggaacggga acgcgcaacc accagtttgc ctttggcatt
      241 gcgcaatgct ttctgtaccc cttgattggc gagttgctcg ctggcggctt gtttgaatcg
      301 attcgatgaa acctgcatga tggtgtcccg gggtcaagcc ctagcgtgtg agttcgtgtc
      361 cacatcgtcc gccatgccgg cgagaacttc ggcaacgtgc cacacacggg tgtcctggtc
      421 gccgcggcgg cgaagccggc cttcgatatt cagcatgcag ccgaggtccc ccagcaccag
      481 tgcgtcggca gctaccgatt gcacatcggc gcatttgaga tcggctatgc gagtggagat
      541 ctccccgtat ttcactgcga acgtgccacc aaatccgcaa cactgttctg gttgttgcat
      601 ttcccggagg ctgaggccat tcactttgga aagcagtgcg cgtggctgct ctttcacgtt
      661 catttcacgc agcccggaac aggagtcgtg ataagtcact gagcccttga actgtcccgg
      721 tacgctggct atccgcgcta cattcaccag aaaatccgtc aattcgtaag tcttgcgcgc
      781 gagcgattcc acggccgcgt tcaactcggg catctctgtg agcaactccg gatagtgcgt
      841 gcgtatcatg ccggcgcaag acccggatgg cactacaacg tattcgcaac ccgcaaattc
      901 atcgatcagt ttttttgcta gcgcttgtgc cgctgctcgg tcgcccgagt tataaccggg
      961 ctgtccacag catgtctggc ccgccggaac gaccacctcg tatccggcca gttccagcaa
     1021 tctcaatgac gcaaacccaa tgctggggcg catcaggtcc accaggcaag ttacaaacaa
     1081 accaactttc atcggacttt caacggttgg gtgcgcgctc tgctatcaga ggcttcttga
     1141 gcacggaagt gcggcgaaga aaggcgcgga tcatagcatg gggcgctttt tgcaccgggc
     1201 cggttgtgac tccgtatccc agcatgtaaa attgccgcat cgcaccattg gacgcatgac
     1261 atggctgaac cgaacggcaa gacgtcaata caggtcatcg aacgcatgat ggacctgctg
     1321 gatgctttgg cccgcaatga agaaccggtg aacctgaagc gtcttgccaa tgccacgagc
     1381 ctgcaccctt ccaccgcgca ccgtatcctc aacatgatgg tggaattccg gatggtggac
     1441 cgcatcgagc cgggcaccta ccggcttgga ataaggctgc ttgaactcgg taaccttgtg
     1501 aagtcccgca ttaacctgcg aaaagaggcg cttccgttca tgcgtctcct gcaccaggaa
     1561 ctgcacgaga cagtcaatct gtccgttcgt cagggagacg agatggtcta tgtagagcgg
     1621 ctgctttccg atcgcacggc catgcgggta gtccatctga tcggtgcgcg tgcgcccctt
     1681 cacgtaaccg ccgctggaaa aatcttcctg cttgaagatg gcgctgaaaa atgccgtgaa
     1741 tatgctcagc ggaccgggct tgaaattttc accaagaata cgattcgcga tctcgctgca
     1801 ttggccaagg aacttgaacg cgtccgaaag ctgggctatg cgcttgataa cgaggaagcc
     1861 gagaacggag tttcctgcat cggcgccggc atttacgacg acgaaggccg ccttgtcgct
     1921 ggattatcgc tttcagcgcc ttccaagcgc atggacaagg gctggtgttc caaggtcaag
     1981 catgtggccg acgaaatctc acgcgcacta gggcatctgc cgggtgcaaa agccgcatga
     2041 caatttggga agccgcttac ttacgatcaa ccggatatgc gcagcaggtc tgcctctatc
     2101 cagcgtttga tgcggtttgc gtcagcaatc cgggagagtt tgccccatga atcgagcagt
     2161 acgatgatca cgggcctgtc tgcaatcatc gcttgcatca ccaggcaccg ccccgcttcg
     2221 ctgatatagc cggtcttcga cagtccgatg tcccattgac ggctcttcac caaccggttt
     2281 gagttgacgt acgacagcaa gcgatgtcgg cgcctgtctt cagaatcgat cttgaatgaa
     2341 tcggaggtcg tgtattcgcg aatgagcggg tagccgtgtg ccgccgcaac catcttggcc
     2401 aagtcttcgg cggtagaaac gttgtctggg gttaacccgg tcggatcctc aaaatgggta
     2461 ttgaccatgc cgatttcgac agctttttga ttcatcgcgg ccacaaaggc ctcggtgccg
     2521 cctgggaaat tacgcgctag cgctgctgca gcacgatttt cggacgccat gagagcgagt
     2581 ttcagcattt cggcacgcat caggctcgtt cctactttga gcttggaccg tgaccccttc
     2641 cggcggtcga catcgctctt gtctatatct atggattcca gcagcggcaa gcccgaatcc
     2701 agcagcacca tcgcagtcat caacttggta atggacgcga tcggcataat ttgatccacg
     2761 ttcttggcga acagcggcaa cccggtgtcc tgcgcaagta cgagcaccgc gccagaacga
     2821 agccgcaacg cggcg
//
LOCUS       DATMZQ010000390         4423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2035618, whole genome shotgun sequence.
ACCESSION   DATMZQ010000390 DATMZQ010000000
VERSION     DATMZQ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035618"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VN496_12555"
     CDS             complement(<1..363)
                     /locus_tag="VN496_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXF17834.1"
                     /translation="MNDQRASFKSMKESTRADWDIIASHHRPYAATLPDRVIAHLKLL
                     GGDTGGFAVDRLQHSLLTATLAHKGGEDEEYVVCALLHDIGDTLASWNHPDVGAAIIK
                     PFVSEANHWMVEKHGIFQG"
     gene            complement(534..779)
                     /locus_tag="VN496_12560"
     CDS             complement(534..779)
                     /locus_tag="VN496_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17835.1"
                     /translation="MDRKLVTAALATTAAALFATTAAIGTASAEDVQVKCYGANACKG
                     HGECKTSMNSCKGKNACKGKSFEMMTEKACIEKVGRA"
     gene            1063..1992
                     /locus_tag="VN496_12565"
     CDS             1063..1992
                     /locus_tag="VN496_12565"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HXF17836.1"
                     /translation="MSLPVIGAPMFIVGVPALVIEQCISGVIGSFPALNARPAEMLET
                     WLLQIESALAAHRKAEPGSKIAPYAVNQIIHQSNDRLEHDLDACIRHEVPIIITSLRA
                     PDDVVKKVHAYGGVVFHDVISIRHAQKALEAGVDGLILVCAGAGGHAGALSPFALLRE
                     VRRFYSGPVALSGSITDGAGILAAEAMGADFAYIGTRFIATREANAVDLYKQMILESK
                     AADVVYTPFFTGVHGNYLKPSIVRAGLDPDNLAPADKISMNFGGGLTKAWKDIWGVGQ
                     GVGNIDDLPPARELVARLHLEYAAARSRLQLAV"
     gene            complement(2034..3665)
                     /locus_tag="VN496_12570"
     CDS             complement(2034..3665)
                     /locus_tag="VN496_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HXF17837.1"
                     /translation="MNEVTKEVLAERVEQRRTFAIISHPDAGKTTLTEKLLLFAGAIH
                     IAGSVKARKATRYATSDWMEIEKQRGISVASSVMQFEYRGKVINLLDTPGHKDFSEDT
                     YRVLTAVDAALMVIDAANGVEAQTLRLLEVCRSRGTPIITFINKMDREVREPLDLIDE
                     IEKALGMSVVPFSWPIGMGKSFRGVYDLAQNRIRVFRPGEDRLDREEEVVTGLDDPSL
                     PPRFGDEFVHARNDMELVQGATPAFSRAEFLAGRQTPAFFGSAINNFGVREVLDALVD
                     IAPPPQPRLALQGPVDPHDPGFSAVVFKIQANMDPAHRDRVVFMRVCSGHFQRGMRLK
                     IHRTGKEIRPNNVVSFLSQRRELLEEAYAGDIIGIPTHGGIQLGDTLTDGLELQFTGL
                     PFFAPELFHIVEVVNPLKTKQLRTGLLQLGEEGAIQVFRPLRNTMMLLGAIGSLQFDV
                     AAHRLKHEYGAEARMSPAPYTCARWVSSDDPVAFAKFVEANAGRLARDAADALAVLTN
                     TTFEMKVIQDRWPDIRFHKSREHAGLLADHIADIV"
     gene            3867..>4423
                     /locus_tag="VN496_12575"
     CDS             3867..>4423
                     /locus_tag="VN496_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXF17838.1"
                     /translation="MAAISIDSQRLWKSLMDLARIGATDKGGVRRLALTKLDGEARDL
                     FIRWCKEAGCSIAIDGIGNIFARRPGCNDALPPVITGSHIDTQPSGGKFDGNYGVMSG
                     LEVVRALNDHKIRTEAPIEVAVWTNEEGSRFTPVMMGSGVYVGAFPLESTLQRSDLEG
                     VTVGQALQQIGYAGPEKIGGRPVGA"
BASE COUNT          864 a         1287 c         1330 g          942 t
ORIGIN      
        1 gccctggaag atgccgtgct tctcgaccat ccagtgattc gcttcggata cgaacggctt
       61 tatgatggcc gccccgacat ccggatggtt ccatgatgcc agcgtgtcgc cgatgtcgtg
      121 cagcagggca cagacgacat attcctcgtc ttctcctcct ttgtgtgcaa gggtcgcagt
      181 cagcagcgaa tgttgcaggc gatcgacggc gaatccacca gtgtctccgc ccaataattt
      241 cagatgcgcg atgacgcgat cgggcaacgt tgcggcgtaa ggccggtgat gggaagcgat
      301 gatgtcccag tccgccctgg tgctctcctt cattgatttg aaactggccc gctgatcgtt
      361 catgttgccc attactgtct ccggaaattg gctgcgtttt cgaaacgaca gtatagcgcc
      421 ggccaaatga cgcagcgctc gtagcatggg gcggtgcagt catgcactat cttctctctg
      481 caaaaaacag agcagggcgc ccgaaggcgc ccccatggtt caaagcggcc tgcttaagcg
      541 cgtccgacct tctcgatgca ggctttttcg gtcatcatct cgaagctctt gcccttgcag
      601 gcgttcttgc ctttacagct gttcatggaa gtcttgcact cgccatgacc cttgcaggca
      661 ttggcgccat aacacttcac ttgcacatct tcggcgcttg ccgtgccgat tgcggccgtt
      721 gtggcgaaca gggcggcggc agtggttgcg agtgctgcgg taaccagttt gcgatccatt
      781 ttgctgactc tcctcaattg ttgcgttagc tcggcccggg acgtggttac ccacgcatgc
      841 cggatgtcat ccgcatcgcc atggtggcgc gcgtaagcga tttatcccgc ggttgcaccg
      901 ggatgcgcgc tcaatgcatc tacggcccca actgccggct ggattcatat taattgcgcg
      961 cggctgcaac caatccttga ctgtgtgaca accggcaggc aagttacgcg gcggattcga
     1021 ttggagagaa ctgtgtcact cgtgaatgaa ctgaagtccc agatgtctct gcccgtgatt
     1081 ggggcgccga tgtttatcgt gggcgtcccg gctttggtca tcgagcagtg catcagcggg
     1141 gtgatcggtt ctttcccggc gctaaacgcc cgtcccgccg aaatgctcga gacctggctg
     1201 ctgcagattg aaagcgcgct ggctgcgcac cgcaaggccg agccgggaag caaaattgca
     1261 ccctatgccg taaaccagat catccatcaa tccaatgaca ggcttgagca cgatctggac
     1321 gcttgcattc gtcatgaagt gccgatcatc attaccagcc tgcgcgcacc ggatgatgtc
     1381 gtaaaaaaag tgcacgcata cggcggcgtg gtattccatg atgtgatcag catccgtcat
     1441 gcgcaaaagg cgcttgaagc gggtgtggat ggactgatac tggtgtgcgc tggagcaggc
     1501 ggccacgccg gcgcactgag tccttttgca ttattgcggg aagtgcggcg tttctattcc
     1561 ggacctgtcg ccctgtcggg atcgattacc gatggcgcag gcatcctggc ggcggaagca
     1621 atgggggcgg acttcgccta tatcggcacg cgcttcattg cgacgcgaga agccaatgcc
     1681 gtggacctct acaaacagat gattctggag agcaaggcgg ccgatgtggt ctacacgcca
     1741 tttttcaccg gcgtgcatgg aaactatctg aagccgagca tcgtacgtgc aggcctcgat
     1801 ccggacaatc tggctccggc ggacaagata tcgatgaatt tcggtggcgg cttaaccaaa
     1861 gcctggaagg acatctgggg cgtcggtcag ggcgtgggca atatcgatga tttgcctcct
     1921 gcgcgcgagc tcgtcgcacg cctgcatttg gaatacgctg ctgctcgttc acgtctccaa
     1981 ctggccgtat aaagaccagc cgccgttttt ggcggacgga tttaaagacg cagctagacg
     2041 atgtccgcga tgtgatccgc cagcagcccg gcgtgttcgc ggctcttgtg gaagcggatg
     2101 tccggccagc ggtcctggat cactttcatt tcgaacgtcg tattggtcag taccgcaagg
     2161 gcatcggcgg catcgcgcgc caggcggccg gcatttgctt ccacgaattt tgcgaacgca
     2221 accggatcgt ccgacgatac ccagcgcgcg cacgtatacg gcgcgggtga catgcgggct
     2281 tccgcgccat attcgtgctt gagccggtgc gccgccacgt cgaactgcaa gctgccgatc
     2341 gcgcccagca gcatcatcgt attgcgaagc gggcgaaata cctggatcgc tccctcctcg
     2401 ccaagttgta gcagtccggt gcgcagttgc ttggtcttca gcggattcac cacctcgacg
     2461 atgtggaaca gctccggcgc gaaaaacggc aggccggtga actgcaattc gaggccgtcg
     2521 gtcaacgtat cgcccaattg aatgccgccg tgagtcggga tgccgataat gtcgccggcg
     2581 taggcttcct ccagcaattc gcggcgctgc gacaggaacg acacgacatt gttgggccgg
     2641 atctccttgc cggtgcggtg tatcttcagg cgcatgccgc gctggaaatg ccccgaacat
     2701 acacgcatga acaccacgcg atcgcggtgt gccggatcca tgttagcctg gatcttaaaa
     2761 acgacggcgg agaatcccgg atcgtgcgga tccaccgggc cctgcaaggc caggcgcggc
     2821 tgcggcggtg gcgcgatgtc cactagcgca tcgagcactt cacgtacgcc gaaattattg
     2881 atggccgagc cgaagaaggc tggtgtttgc cgcccggcca ggaattctgc tcgtgagaac
     2941 gctggcgtgg cgccctgcac cagttccatg tcgttacgtg catgcacgaa ttcatccccg
     3001 aagcggggtg gcagtgaggg atcgtccagt ccggtgacga cctcttcttc gcgatccagc
     3061 cggtcttcgc caggacgaaa tacgcggatg cgattttgcg caaggtcata cacgccgcga
     3121 aaggatttgc ccatgccgat tggccaggaa aacggcacga ccgacatgcc gagcgccttt
     3181 tcaatttcat cgatcaggtc caagggctcg cgtacttcgc ggtccatttt gttgatgaac
     3241 gtaatgatcg gcgtgccgcg cgaacggcac acttcgagca ggcgcagcgt ctgtgcctcc
     3301 acaccgttgg cggcatcgat taccatcaat gctgcgtcca ctgcggtcag cacgcgataa
     3361 gtgtcctcgg aaaagtcctt gtgtccgggg gtgtccagca agttgattac cttgccgcga
     3421 tactcaaatt gcatcacgga actggccacc gaaatcccgc gctgcttttc gatttccatc
     3481 cagtccgagg tggcgtagcg cgtggccttg cgtgccttta cgctaccggc gatatggatg
     3541 gcgccggcaa acagcagcaa tttttccgtc aacgtggttt tgccggcgtc cgggtgcgaa
     3601 atgatcgcga acgtccggcg ctgttcgacg cgctctgcca gcacttcttt tgttacttcg
     3661 ttcatgaagg cttcaaggga tgcctgtttg tcgcacgtgg agacgtgcgc agccaccgac
     3721 ccctgcaaaa ggggcgcatt ttacgggctt ggcttgaacg cgtccataat ctgtccagcc
     3781 agttttagaa cctggtatcg ggacgaacgc atccgaacaa ccgcaatccg gcagggcctg
     3841 gagtggctca tttgaaggaa ccagacatgg ctgcaatatc gattgattcc caacggttgt
     3901 ggaagtccct gatggacctg gctcgcatcg gcgccaccga caaaggcggc gtgcgccggc
     3961 tcgcgctcac caagctggac ggcgaagcgc gcgacctttt cattcgctgg tgcaaggaag
     4021 caggctgcag catcgcgatt gatgggatcg gcaacatctt tgcgcggcgg cccggatgca
     4081 acgacgcgct gccgccagtt attaccggga gccacatcga cacccagcct tccggcggga
     4141 agttcgacgg caactatggc gtgatgtccg ggctcgaggt ggtccgcgcg ctgaacgatc
     4201 acaaaatccg cactgaagcg ccgatcgaag tcgcggtgtg gacgaatgag gaaggctcgc
     4261 gctttacgcc ggtgatgatg ggatcaggcg tttacgtcgg tgcgtttccg ctggaatcaa
     4321 ctttgcagcg ctctgacctc gaaggcgtga ccgtcggcca ggcgctccaa caaatcggct
     4381 atgccgggcc ggaaaaaatc ggcggccggc cggtcggcgc cta
//
LOCUS       DATMZQ010000391         5163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2226552, whole genome shotgun sequence.
ACCESSION   DATMZQ010000391 DATMZQ010000000
VERSION     DATMZQ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226552"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VN496_12580"
     CDS             <1..859
                     /locus_tag="VN496_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17839.1"
                     /translation="VYTVGEYVGLAFLSPMLFWILGAFGWRTLFFTVGLAGILFGLFW
                     WRTYREPHESKNVNQSELDYIAAGGGIVTPKSTTKFEWHLIAKLLRFRQLIGICIGQF
                     AGNSTLVFFLTWFPTYLATERHMGWLKIGFFTVLPFIAASIGVLFGGWWSDWMLRRKR
                     SANMARKLPIIIGLLLASVIVTANYVESDHVVIAILSLAFFAQGMAALGWTLVSDIAP
                     KGLLGLTGGIFNFAANLAGIVTPLVIGFIVAATGSFFGALAFIAVVALIGAFSYIFIV
                     GDIRRIELD"
     gene            944..1714
                     /locus_tag="VN496_12585"
     CDS             944..1714
                     /locus_tag="VN496_12585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17840.1"
                     /translation="MTRVANKVVVLTGAGSGIGLAAAKLFAREGARLMLAGRNGAALA
                     EAVKTVGEDKAAYMVADVSQPEDNLRLIGAAEQRFGGIDSFIANAGVEGATLPIADYP
                     LDVFDKVIAINVRGVFLGLKYAIPALKKRAGGSIIITSSIGGIKARGQGNSAYIASKH
                     AEIGLMRAASVECAPFNIRVNCVLPGPTDTRMMRNIEEGRSPGAPDKARDIILAGLPL
                     RRYGTADEIARLMLFLISDESSICTGGVYAADGGLSAI"
     gene            1826..2407
                     /locus_tag="VN496_12590"
     CDS             1826..2407
                     /locus_tag="VN496_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HXF17841.1"
                     /translation="MNLQNTSRLVGALLSVFVSLVIISPAHAGEYTDTINMFKKAAES
                     GTFFQNGYGYAVFPTVGKGGIGIGGAFGKGRVYKKGKYIGDTSVSQLSVGLQFGGQAY
                     SQIIFFEDERSLQEFTSGNFEFGAEASAVAITAAAGAKTSTTGTSVNASGNKNDATVA
                     GQYQKGMATFTVAKGGLMYEATIGGQKFSYKAR"
     gene            2571..3446
                     /locus_tag="VN496_12595"
     CDS             2571..3446
                     /locus_tag="VN496_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXF17842.1"
                     /translation="MTRKVQLPIRICTAFTALLASFLLSGAPAHAQETQGVQRMYVLN
                     CGESETNDLSAWSPDVNIGKHWEFSDSCYLIRHAKGLLLWESGVSDAIAAMPDGFVVG
                     NGRLTLHVRKTLRSQLQELGISPADVTHLGFSHFHGDHVGNANLFTAATLYIQQAEYD
                     AAFGPDPSKFGFNPAFYNKLRDSTVMKLDGDHDVFGDGSVMIYSTPGHTPGHQSLLVR
                     LRHTGPVLLSGDAVHFRENWQNRRVPARNFNKEQSLASMDKLAAILVREHATLWINHD
                     KEQTDTLAHAPAFLE"
     gene            3581..4804
                     /locus_tag="VN496_12600"
     CDS             3581..4804
                     /locus_tag="VN496_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF17843.1"
                     /translation="MPSLSLSASAEKSHSPPPTLQWTESGREYSARWRSERRTPPPKR
                     VLPVDDTITADAAYRLACEGTALLWRGDFHNATQLLHAMARRFDKTPARKRKKKLQAQ
                     SHAATPSDAFHQYRQVQSQRTRVLGSLLIPFNADCSIALRRAPDVRKACIEAWGPVEG
                     AAEATVASLRELLGIIGAHEWREKGVEIRALGSPPNNRIHPHYGVFSPVRGEYVDLVA
                     QAALPLTGQEPTVAFDIGTGTGVLAAVLARRQVDVVIATDHDPRALACARENITRLGL
                     EAQVRVVHADLFPEGHASLIVCNPPWLPARPGSRLESTVYDEDSRMLLGFINGLAAHL
                     EAAGEGWLILSDLAEHLALRSRAALLAAIERAGLRIIGRIDTKARHPKAADASDPLHA
                     ARAAEVTSLWRLARA"
     gene            4883..>5163
                     /locus_tag="VN496_12605"
     CDS             4883..>5163
                     /locus_tag="VN496_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXF17844.1"
                     /translation="MSEILSPEGILAQEFHTLPELIRLHAAQRPHHIALRQDERTLDY
                     RSLDELADRVAATLQRAGLGPGRTIAICAAMSIEYAAVFIGALRAGIAVA"
BASE COUNT         1043 a         1462 c         1559 g         1099 t
ORIGIN      
        1 ggtttacacc gtgggggaat atgtcggcct cgccttcctg agtccgatgt tgttctggat
       61 actcggggcc ttcggctggc gcaccctgtt cttcaccgtg ggcctggccg gcattctatt
      121 tggcttgttc tggtggcgca cttaccgcga accgcatgaa agcaagaacg tgaaccagtc
      181 tgaactggac tatatcgccg cgggcggcgg cattgtcacg ccgaagtcca cgacgaaatt
      241 cgagtggcac ctgattgcaa agctattgcg gtttcggcag ttgatcggca tctgtattgg
      301 gcaattcgcc ggcaactcca ccctcgtgtt cttcctgacg tggttcccga cttacctcgc
      361 cacggagcgc cacatggggt ggctgaagat tggtttcttt actgtgctgc cgtttatcgc
      421 cgcttccatc ggggtactgt ttggtggctg gtggtcagat tggatgctta ggcgcaaacg
      481 gtcggcgaat atggcgcgca agctgcccat catcatcggc ctgttgctgg catcagtgat
      541 cgtcaccgca aactatgtcg aaagcgacca tgtcgtgatc gccattcttt cgctggcgtt
      601 ttttgctcag ggcatggcag cgctgggctg gacgctggtt tcagacatcg cgcccaaggg
      661 cttgctcggg ctgactggcg gaattttcaa cttcgcagcc aatctcgctg gcatcgttac
      721 gccgctggtg attggtttta ttgtcgcggc caccggatcg ttcttcggag cgctggcatt
      781 tatcgccgtg gtggctttga tcggtgcctt ttcctacatt ttcatcgttg gcgatatacg
      841 ccgtatagaa ttggactagc tgccggactc atggacaccg gctttggcgg tatccaggcc
      901 gtgatccgat caatgaagcg cgacagcatc gtgtgagaac cccatgacaa gagttgccaa
      961 caaagtggtg gtgctgactg gtgcaggtag cggcatcgga ctggcggccg ccaaattgtt
     1021 cgcgcgggaa ggtgccaggc tgatgctggc cggacgcaat ggagcagcac ttgccgaggc
     1081 ggtgaagact gtaggcgagg acaaggccgc atacatggtg gccgacgtat cgcagcccga
     1141 agataattta aggctcattg gtgcggccga acagagattc ggcggcatcg acagcttcat
     1201 tgccaacgcc ggcgtcgaag gcgcgacgtt accgatagct gattacccgc tcgatgtgtt
     1261 cgataaggta atcgccatca atgtgcgcgg cgtattcctt ggcctcaagt acgcgattcc
     1321 cgcgctcaaa aaacgggcag gtggcagcat catcatcaca tcgtcgattg gtgggatcaa
     1381 ggcacgcggc cagggcaact cggcctatat cgccagcaag cacgccgaaa tcggtttgat
     1441 gcgggccgcg agcgtggaat gcgctccgtt taatatccgc gtaaattgcg tactgccggg
     1501 tccaaccgac acccgcatga tgcgcaacat cgaggaaggc cgttctccgg gagcgccgga
     1561 caaagcccgc gatatcattc tcgcgggact gccgctcagg cgctacggta ctgccgacga
     1621 aattgccagg ctgatgttgt tcctcatctc cgacgaatcc agtatctgta ccggtggggt
     1681 gtacgccgcc gacggtggat tgtcggcgat ctagcgagtt ggacgggcgt cgataggccg
     1741 caaggtgcgc acgcgttaca ctggctcatt gatttatctt atcgcccgcg atcgacaaca
     1801 aaacttcagg aagggacaaa aaatcatgaa tttgcaaaat acaagcagac tggtcggcgc
     1861 gcttctctct gtgttcgttt ccctggtgat catttcgccg gcccatgccg gggagtatac
     1921 ggacaccatc aacatgttca agaaagcggc cgagagcggc acgttcttcc agaacggcta
     1981 tggctacgcc gtgttcccta ccgttggtaa aggtggcatc ggaattggcg gcgcgttcgg
     2041 caagggccgg gtgtacaaga agggcaagta catcggcgac acgtccgtat cacagctatc
     2101 ggtggggttg cagtttggcg ggcaggccta tagtcaaatc atatttttcg aagatgaacg
     2161 ctcgctgcag gaatttacga gtggcaactt cgagttcggc gccgaggctt ctgccgttgc
     2221 tatcaccgct gcagcgggcg ccaagacaag caccacaggg acatcggtga acgcgagcgg
     2281 taacaaaaac gatgctacgg tcgccggcca ataccagaaa ggtatggcga cattcaccgt
     2341 ggcgaagggt ggattgatgt acgaagcgac catcggcggg caaaagttca gctataaggc
     2401 acgctgacgg cttgctccag cgtcgacgat agcgatgcga tcgcaaggcc ggtcgatatc
     2461 gcagaacacc gaccggtaac tttggtgcct ggcttgttga ggttggggct gacgtgcagg
     2521 ccgtaattcg tgtcgagcgc gtaactaact gatcggccgg agaagaaatc atgaccagaa
     2581 aagtgcaatt accgattcga atctgcaccg ccttcactgc cttgcttgca agtttcctgc
     2641 tgagcggtgc acccgcgcat gcacaagaga cacagggagt gcagcgcatg tacgtgctga
     2701 attgcggcga gagcgagacc aacgatcttt cggcctggtc gccggacgtc aatatcggca
     2761 agcattggga gttcagcgac agctgctatc tgatacgcca tgcgaaagga ttattgctgt
     2821 gggaatccgg cgtctcggat gcaattgcgg caatgccgga cggttttgtg gtcggcaacg
     2881 gcaggctgac cttgcatgtg cgcaagactt tacgctcgca attgcaggaa cttggcatct
     2941 cgccggcaga cgtcacacat ctgggttttt cccatttcca cggcgaccat gtcggcaatg
     3001 ccaatttgtt taccgcagca acactctaca tccagcaggc cgagtacgat gcagcctttg
     3061 gccccgaccc ttccaaattc gggtttaatc ccgccttcta caacaagttg cgcgacagca
     3121 cggtaatgaa gctggacggt gaccacgatg tttttggcga cggctcagtg atgatttatt
     3181 cgacacccgg ccatacgccg gggcatcaat cgctgctggt gcggctgcgc cacaccgggc
     3241 cggtcctgct gtctggcgat gcggtgcatt tccgggagaa ctggcagaac cgccgcgtgc
     3301 cggcacgtaa tttcaacaag gaacagagcc tcgcatccat ggataagctg gcggccatcc
     3361 tggtcaggga acacgcaacc ttgtggatta accacgacaa ggagcagact gacacgctcg
     3421 cgcatgctcc ggcgtttctc gagtgaggga gcagctcgtc cggcgcggct aagttcgctg
     3481 ttgccgtaag agggcggacc cgtatctctg cgtgcaatgg caatccgctg cgcttgttca
     3541 tgaaagagca ttgcgggttt acaggctgcc tggaaattga atgcccagtt tgtctttgtc
     3601 ggccagtgct gaaaagtccc acagtccgcc gccaacgctt cagtggaccg aatcaggccg
     3661 ggaatattca gcgcgctggc gttcggaacg ccgaacgccg ccaccgaagc gcgtgcttcc
     3721 ggtggacgac accattacgg cagatgcggc gtaccggttg gcctgtgaag ggacggcatt
     3781 gctctggcgt ggagatttcc acaacgccac acagttgctg cacgcaatgg cgcgccgctt
     3841 cgataaaacg ccagcacgca agcgaaaaaa aaagctgcaa gcgcaatctc atgcggcaac
     3901 tccctccgac gcctttcatc aatatcggca ggttcagtcg cagcgcacgc gtgtcctcgg
     3961 ttcgctgctg attccattta atgcagattg cagtatcgca ttgcgccgag ctccggatgt
     4021 gcgcaaagcc tgcatcgaag cgtggggccc ggtcgaaggg gctgcagagg cgacagtggc
     4081 ctcgttgcgc gaactgctgg gaataatcgg cgcccatgag tggcgggaaa aaggtgtcga
     4141 aatccgcgct ctcggcagcc ctccaaacaa ccgtattcat ccacattacg gcgtcttttc
     4201 tccggtgcgc ggcgaatatg tggaccttgt cgcacaggct gccttgcccc tgaccgggca
     4261 ggaacccact gtcgcttttg acattggcac gggcaccggc gtgctggcgg ctgtgttggc
     4321 acgccgacag gttgatgtgg tgatagccac cgatcacgat ccccgtgcgc tggcctgcgc
     4381 gcgcgagaac attacccgac ttggcctgga ggcccaagtg cgcgtcgtcc acgcagacct
     4441 tttcccggaa ggccatgcgt ctctcatcgt gtgcaatcca ccgtggctgc cagcgcggcc
     4501 aggttcgcgg ctcgagtcca cggtctatga cgaagacagc cgcatgctgc tcggttttat
     4561 caatggactg gccgcgcatc tggaggctgc tggcgagggc tggctgatct tgtcggacct
     4621 tgccgaacat ctcgcattgc gttcgcgcgc cgcgctgctg gcggcaatcg agcgcgccgg
     4681 gttgcgcatt atcggacgaa tcgataccaa ggcacgccac cccaaggccg cagatgcgag
     4741 tgatccgttg catgccgcgc gtgcggctga agtcacttcg ctctggcgcc tcgcgcgcgc
     4801 ttagtgcgat ggcttggaca tctgaacggc gttcccgata tcatccgcac gacttcaata
     4861 catgactgcc cgtgactcct gaatgagtga aattttgagc ccggaaggaa ttcttgcgca
     4921 ggagtttcac acgctgccag agctcatccg cttgcatgct gcgcagcggc cgcaccacat
     4981 cgcactgaga caggacgagc gcacgctgga ctatcgatcg ctggatgaac tcgcggaccg
     5041 cgtcgcggcc acgctgcaac gtgccggctt gggccctggc cgcaccatcg caatttgcgc
     5101 cgcgatgtca atcgaatacg ctgcggtatt catcggtgcg ctgcgcgcgg gtatcgcagt
     5161 ggc
//
LOCUS       DATMZQ010000392         5357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4298130, whole genome shotgun sequence.
ACCESSION   DATMZQ010000392 DATMZQ010000000
VERSION     DATMZQ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4298130"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="VN496_12610"
     CDS             complement(<1..2087)
                     /locus_tag="VN496_12610"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXF17845.1"
                     /translation="MSAGALPRVARTSTFAPSINDWRKTLANGRANLKTSFAAKPDAG
                     KSLQNQCALVDAALQGIWTETGQSDELALIAVGGYGRGLLYPYSDVDVLILLPGEIEE
                     TVRGQVEALVGVLWDIGLEIGHSVRTIEQCQDEARRDITVQTTLLESRLVTGSRKQYT
                     RFKNAIFSDLDVDYFVEAKLLEQQQRHTRFNDTAYNLEPNVKESPGGLRDLQNILWIS
                     RAAGLGHTWQDLAREKILTREEVRQLRRHEAMLAVLRVRLHYLTNRREDRLLFDFQSA
                     LAKEYGLADTPARRASEQLMQQFYTTAKAVQLLNTIVLQNLGKRIFPVASTQPRELSE
                     RFRINSELLEASHGLFQRQPSAILEAFLLLQDHPELKGFAVPTMRALWHARDQIDAAF
                     RRNPENRALFMQILRRPTGITHALRRMNQYGVLGRYLPAFGRIVGRMQHDLFHVYTVD
                     EHILMVVRNLRRFTVPEMAHEYPLCSRLISEFDRIEVLYLAGLFHDIAKGRGGDHSIL
                     GMADASSFCRAHGVSKEDTALVTWLVEQHLMMSATAQKQDLSDPDVIRAFANKVQNQR
                     RLIALYLLTVADIRGTSPKVWNAWKAKLLEDLFHLTRRLLDPGQMPADHTLQARQERV
                     LVKLRAYAIADEASRRFWQKLDDSYFLRHDEPEIVWHTRLLNYRADAAVPVVKARLSP
                     VGEGLQVMIYAPD"
     gene            complement(2088..2888)
                     /gene="map"
                     /locus_tag="VN496_12615"
     CDS             complement(2088..2888)
                     /gene="map"
                     /locus_tag="VN496_12615"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXF17846.1"
                     /translation="MMSVPIKTPEEIEKMRVAGRLAAEVLDFIAPHVRPNVTTEELDA
                     LCHDYMVNVQHTIPAPLNYAPPGYQPYPKSICTSVNHVVCHGVPGAKKLKAGDVLNID
                     ITVIKDGFHGDTSRMFFVGEPSIQARRLAQITYECMWRGIDAVKPGAYLGDIGEVIQA
                     HAEEHGYSIVREFCGHGIGRNFHEEPQVLHYGHAGTGIRLQPGMVFTVEPMINAGKAG
                     IRQLADGWTVVTKDHSLSAQWEHTVLVSDTGFEVLTVSPGTPPPPATQ"
     gene            complement(3173..3664)
                     /locus_tag="VN496_12620"
     CDS             complement(3173..3664)
                     /locus_tag="VN496_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17847.1"
                     /translation="MKTIKDSYGTKHAAEANASRHPTDFPSEEDILTFAPEGWTDYIR
                     QLGTDFIAQVSIYNEVADLVDALNEQSTEAEPAPNPIGHSRIDWLAGPRVLLLENADL
                     GVCWMFWVGSEWTPMDSASGTHGSHVKRRVLQLADVLACIKIMSPGAYSEHLEWEMER
                     SKH"
     gene            3892..3967
                     /locus_tag="VN496_12625"
     tRNA            3892..3967
                     /locus_tag="VN496_12625"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3925..3927,aa:Asn,seq:gtt)
     gene            complement(4245..4577)
                     /locus_tag="VN496_12630"
     CDS             complement(4245..4577)
                     /locus_tag="VN496_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17848.1"
                     /translation="MALSLELERWINSWTNRRNGEVGILLALCKQHERCRNTPQTRRH
                     MPADHRKNAPDKSDPTRKTGQNNAKPKNPADARHDKSQQAENRKELGVGEDHKTKDME
                     QKNRGTFP"
BASE COUNT         1094 a         1517 c         1568 g         1178 t
ORIGIN      
        1 tcgtccggtg cgtaaatcat gacctggagg ccctctccga ccggagacag ccgcgctttt
       61 acgacaggaa ccgctgcatc ggcacgatag ttcaacagcc gcgtgtgcca tacgatctcc
      121 ggctcgtcat ggcgcaggaa atagctgtcg tcaagttttt gccagaatcg gcgcgatgcc
      181 tcgtccgcga tggcgtaggc gcgcagcttc accagcacac gctcctgacg agcctgcagc
      241 gtgtggtccg ccggcatctg tccgggatcg agcagccggc gagtcaagtg aaacaagtcc
      301 tcgagtaatt tggctttcca cgcattccac actttggggc tggtaccgcg tatgtccgct
      361 acggtcagta aatacaacgc gatcagcctg cgctggttct gaactttgtt ggcaaatgca
      421 cgaatcacat cgggatccga cagatcctgc ttttgtgccg tggccgacat catcaagtgt
      481 tgctcgacca gccaggttac cagcgcggta tcttctttcg atacgccgtg cgcccggcaa
      541 aaactcgagg catcagccat accgaggatg gaatgatcgc cgccccggcc tttcgcgata
      601 tcgtgaaaca gacccgcgag atacaagacc tcgatacggt cgaattccga gatcagccgg
      661 ctgcacaatg gatattcgtg cgccatctcc ggcacggtga agcggcgcag attgcgcacg
      721 accatcagga tgtgctcatc aacggtgtaa acatggaaca gatcatgttg catgcgcccg
      781 acgatgcggc caaacgctgg caggtagcgg cccagaacgc cgtattgatt catccggcgc
      841 agcgcgtgcg taataccggt agggcgtcgc aagatctgca tgaacagcgc gcgattttcc
      901 gggttgcgtc tgaaagcggc atcgatctga tcgcgcgcgt gccacaaggc gcgcatggtc
      961 ggaacagcaa agcctttgag ctccggatgg tcttgcagca acagaaaggc ttccagaatc
     1021 gccgagggtt ggcgctggaa caaaccgtga ctggcctcga gcagttcgga attgatacga
     1081 aagcgctcgc tcaattcacg cggctgggtg gacgccacgg gaaagatgcg ctttccgagg
     1141 ttctgcagca cgatggtatt gagcaactgg accgcttttg cggtggtata gaactgttgc
     1201 attagctgtt cgctggcgcg cctggccggc gtatccgcca aaccgtattc cttggccagc
     1261 gctgactgga aatcgaacag caggcgatct tcgcgccgat tcgtcagata gtgcaagcga
     1321 acgcgtagca cggcgagcat ggcttcgtga cgcctcagct ggcgcacttc ttcacgtgtc
     1381 aggatcttct cgcgcgcgag gtcctgccag gtgtgtccga gacctgcggc gcggctgatc
     1441 cacagaatgt tttgcaagtc acgcagtccg ccagggcttt ccttgacatt gggttcgagg
     1501 ttatacgccg tatcgttgaa ccgcgtgtgc cgctgctgtt gttccagcag ttttgcttcg
     1561 acgaaataat cgacatcgag atcgctgaaa atggcattct tgaaacgcgt gtactgcttg
     1621 cggctgccgg tgacgagccg cgattcgagc agcgtggtct ggacggtgat gtcgcgccgt
     1681 gcttcgtcct ggcattgctc gatggtgcgc acgctatggc cgatttccag accgatgtcc
     1741 catagcacgc caaccaaagc ctcaacttgc ccgcgtacgg tttcttctat ttcgcctggc
     1801 agcaggatca gcacgtcgac gtcggaatac ggatagagca ggccacggcc gtagcccccg
     1861 acggcgatca acgcgagttc atcggattgg ccggtttcag tccagatgcc ctgcagagcc
     1921 gcgtcgacca gtgcgcattg gttttgcagg gacttgcccg catcgggctt tgcggcgaac
     1981 gaagttttga ggttggcgcg gccgtttgcg agtgtcttgc gccagtcatt gatactgggc
     2041 gcaaacgtgc tggtccgcgc gacgcgcgga agggccccgg ccgacatcta ttgcgtcgcc
     2101 ggcggcggtg gcgttcccgg cgagacagtc aatacttcga agcctgtgtc ggagacgagc
     2161 acggtgtgtt cccactgcgc cgacagggaa tgatctttag tgaccacggt ccagccgtcc
     2221 gcaagctggc gaatacccgc cttgccggca ttgatcatgg gctccacggt gaacaccatg
     2281 cctggttgca gccggatgcc agtcccggca tggccgtaat gcagaacttg cggctcttca
     2341 tgaaaattcc tcccgatgcc gtgaccgcaa aactcacgca cgatggagta gccgtgttct
     2401 tcagcgtggg cctggataac ctcgccgatg tccccgaggt aggcgccggg tttcacggcg
     2461 tcgattccac gccacatgca ttcgtaagtt atttgtgcga gccggcgcgc ttgtatggag
     2521 ggttcgccca caaaaaacat gcggctggtg tcaccatgaa agccgtcctt tatgacggtg
     2581 atatcgatat tcagaacatc gcccgctttg agctttttcg ctcccggcac gccatgacag
     2641 acgacatggt taaccgacgt gcaaatggat ttcggatatg gctggtagcc gggcggcgca
     2701 tagttcagcg gcgccggtat ggtgtgctga acattgacca tgtagtcatg gcacagcgca
     2761 tcgagctctt cggtcgtgac attgggccgg acatgcgggg cgataaaatc gagtacctcg
     2821 gccgcgaggc ggcctgctac acgcattttc tcgatttctt caggcgtttt gatggggacg
     2881 gacatgatta acaaatcgtt aagccgctaa tgataacacc cgggtccgac tgcttcggtg
     2941 gtctgggtac tcagcaaatg aagctccatc ccctctaatt caaccatcac cacaggcggg
     3001 aattgtttga tcgatgcacg atggcaagcc gcacgctgcg aaggtaagcc ttccggtatt
     3061 ggctccgttt agcgcacagc aggaggagtt gcggggaagt tgcgggaccg gcatacggag
     3121 cagctgccca tgcagcggct cgttggaggg acaacagcaa agcagtggac agctagtgct
     3181 tcgagcgttc catctcccat tccaggtgtt cggaatacgc accgggcgac attatcttta
     3241 tgcacgccag tacatcagcc aactggagca cgcgacgctt gacgtgcgag ccgtgcgtac
     3301 cgctggcgga atccatgggc gtccactcgc taccgaccca gaacatccag caaacgccca
     3361 ggtcagcgtt ctcgagcaac agcacgcgag gccctgccag ccagtcaatg cggctgtgcc
     3421 cgattggatt tggtgctggc tcggcttcag tggattgttc gttgagggca tccaccagat
     3481 ctgcgacttc attgtatatg ctgacctgcg caataaagtc agtgccaagc tggcggatat
     3541 agtcggtcca gccctcgggc gcgaaggtga gtatgtcttc ctcggacgga aagtcggtcg
     3601 gatgccggct tgcattggct tcagcggcat gcttcgtgcc gtaactgtct ttgatcgtct
     3661 tcacgccact gccttcggag cgcgttgcgc gcgacctgac cggcgaagca atgcagggct
     3721 ataacgatga agaggtaaga ccccgacgct gctccggata taaatcccgc ctgccaaagc
     3781 gtcccggaat agcatcgtcg gcgtttgcga cgcatcccgg tatcgggtaa tatgcgcgcc
     3841 tttcaagcgc tggacgagtg aagggaaacc cagcgtctgg cagaaaagta ttccctgata
     3901 gctcagtcgg tagagcgacg gactgttaat ccgcaggtcc ctggttcgag tccaggtcgg
     3961 ggagccaaca ccttcgaatg atagaaacac gtcctgtccc gggcattgtt cgccgcattg
     4021 ctttgcacca ccttcctggc ggcctcaagt gatcggcata ctcacgcagc ttacttagct
     4081 tgcgcccgtt ccagattgat gtggaatagt ccgaaaggac gaatagcaag cggcaaggac
     4141 tgtaagcacc ccgtgaattc gatgttgcgc cggttgagct catgctttgc ggtatgtggg
     4201 cgaatggaac tggggaagcc gccgcgccgc gcaaaaactg cgttctacgg aaaagtaccg
     4261 cgattctttt gttccatgtc ctttgttttg tgatcctcgc ctacccctag ttcctttcga
     4321 ttttccgctt gctgtgactt gtcgtggcgc gcgtcagcag ggtttttcgg tttggcattg
     4381 ttctgcccgg tttttcgagt tgggtcggac ttgtccggcg cattcttacg atggtcggct
     4441 ggcatatgtc tccttgtctg tggggtgttg cggcagcgtt cgtgctgctt gcaaagtgca
     4501 aggaggatgc caacttcgcc gttccgcctg ttagtccagg agttaatcca gcgctccagt
     4561 tcaagtgaca gtgccattgg ctggatctga aatttaccga tctgtgtaca aattactgcg
     4621 gcgatgcctc tccagcaaaa cgtataagaa ccgcaagtcg gctgcggaga tatgcaaaac
     4681 agccttttca tccgctgcgc caatggcaca acgcttgcat cagaacatta cccagatccc
     4741 gcgcaggtcg cgacatctct tgggcaatcg cggcatccca gacaagatcc ccaatgaagg
     4801 cgcctcgtgg gccgtgaatt gccggcaggt cgactcactc tcaaatgcaa aaagtcgcgc
     4861 agtggcgctt cgaaagagtt gccgagcaca gctggtactg ggagcgtatc ggagctgagt
     4921 ctggtacgag aatcgggccc ttttcgagct tcaccgcctg cctcatggat gcccggcaaa
     4981 agggattcaa ccccgggatg ccggaaagaa ggcggattcc aagagatccg cagccgcctt
     5041 tgttcagccg ctgaagctgc cgaaaaaaag ggcaccctcg cggggtgccc ttcaacattc
     5101 atcattgggg tgactacacg ttgcttggcc ccttcgggct gttagcaggg gttggccacc
     5161 acctagaaga gcgtaaggtt atctcctacg cttgtcagaa ttctcctgca ttcgaggcaa
     5221 attctcaatg gcacgcgcct ggtccgtccg gactatggcc aaggtacacg acgctctctg
     5281 caccgtcaca tatgtaacgc tgaacggtca accgactacc tttgcgatag cgcctgtaac
     5341 atggtctatg ttgcggg
//
LOCUS       DATMZQ010000393         5729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3608512, whole genome shotgun sequence.
ACCESSION   DATMZQ010000393 DATMZQ010000000
VERSION     DATMZQ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608512"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1194
                     /locus_tag="VN496_12635"
     CDS             88..1194
                     /locus_tag="VN496_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXF17849.1"
                     /translation="MAEQIDCAVIGAGVVGLAIARELAMAGREVIVLEAADAIGTHTS
                     SRNSEVIHAGLYYPKGSLKARFCVTGKGLLYEYCSVHGVPHSNLGKIVVAVTADEVTI
                     LKSYVEKAQQNGVTDLRWLSVEALRELEPAVECVAGFHSPSTGIIDSHALMLAYQGDA
                     ESCGASVVFKSRVESGEVTGEGIVLNVSGEESMSIACQSVVNSAGLFAQDVARSVQGI
                     PKSSIPPQYFAKAHYYTLSGKAPFRRLVYPVASHAHLGVHVTVDMAGQIRFGPDVSWV
                     NGVDYTFDVSREPLFYEAIRKYYPALKQGQLQPGYTGIRPKVSGPKEPAADFIIQGPK
                     HHGVAGLVNLYGIESPGLTASLAIAQHVKQLLQG"
     gene            1222..2529
                     /gene="paaK"
                     /locus_tag="VN496_12640"
     CDS             1222..2529
                     /gene="paaK"
                     /locus_tag="VN496_12640"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HXF17850.1"
                     /translation="MGAMDQLEPIEKAGVDELRTLQLERLRWSLLHAYENVSHYRKAF
                     DAAGAHPSDLKQLSDLERFPFTTKTELRENYPFGMFAVPMNDIVRVHASSGTTGKPTV
                     VGYTKKDIGMWAAVMARSLRAAGVKSSDIVHIAYGYGLFTGGLGAHYGAEALGATVIP
                     SGGGMTERQVRLICDFKPDVIMCTPSYLLVIADEFARQGLDPAQSSPRIGIFGAEPWS
                     ESMQAELERCFGIVALDLYGLSEVIGPGVAAECIESRDGLTIWEDHFYPEIIDPRTGA
                     VLPEGATGELVFTSLTKIGMPVIRYRTRDLTRLLPGTARTMRRMARVTGRSDDMLIIR
                     GVNVFPSQVEEILVQQSALSPHYVLEVTRDGHMDELDVLVERRADIPGPLAATARRSI
                     EQEVERMIKGLIGVTTKVRVAEPQTLPRSQGKAARVIDKRPRN"
     gene            2540..3946
                     /locus_tag="VN496_12645"
     CDS             2540..3946
                     /locus_tag="VN496_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXF17851.1"
                     /translation="MITSSGLTPAEPLLKVNLENEAVAGWLASSPRDVGDFSGDCIRY
                     SRFWQASATLLGRLPAKPERNAQQAEAAKSIHGLAREARARFLSAHSGALYDRLTRSR
                     TRFVRVEQLVYDAAELVPGLTPTQAQVDGELALMQRDKEGHEIDQGILISHVLADQSA
                     GTHLCHAMLLPRPEAIERLAEFQANGRIDLGAAEVFRNGKAAYVIQKNPRHLNAEDDS
                     TIDAAEIAVDLAILDPQSQIGVLRGDYVQGGKYDGRRMLGSGINLTHLYYGKIPYIWY
                     LQRDLGIVNKFFRGVAREDVIPDDITGETNEKPWIAVVEQFAIGGHCQYLLTMDYVLA
                     ARGAFMTLPARKEGIIPGAANMRMPRFTGERIARQAILYGLRLECDSTEGRLMCDEIV
                     APEEMDAAIERVVKNFTGSGIVSAASNRRAFRVTQEPLDLFRRYFATYAREQAYCHFS
                     PALIENLETHWNAKTRKL"
     gene            4119..4244
                     /locus_tag="VN496_12650"
     CDS             4119..4244
                     /locus_tag="VN496_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17852.1"
                     /translation="MTAAIMIIGPLSVVIGCLYWVMSLGKAQATLNADLWPKISK"
     gene            complement(4314..>5729)
                     /locus_tag="VN496_12655"
     CDS             complement(4314..>5729)
                     /locus_tag="VN496_12655"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXF17853.1"
                     /translation="RDQTNHAELAFDGEGKILGLRVDIVGNIGAHVSGPGAVIVVAVG
                     PKVITGVYHVPTLHLRGTAVMTNTNVVGAYRGAGRPEAIYLLERLMDQAAAEMKMDPA
                     ELRRRNFIQPSQMPYKTPMGEKFDSGNFPHMLDRILQQADWKGYAARREESRKRGKLR
                     GRALSTFLEWTGVVHEESVNLHVEADGRVLVYTAMQAMGQGIETSYVQILSETLAIDP
                     ERIVIVQGDSDIGQGIGSMGSRSLYIGGSAMLTASKQAIEKGRELAGDALEAAGQDIE
                     YRDGSFKVAGTDLGIGLFELAGQQPEKRIAISTLQKVDGPSWPNSCHVCEVEIDADTG
                     VTQIVRYTTVDDVGRVINPLIVAGQVHGGIAQAVGQALLENAHYDPQTGQLITGSLQD
                     YCLPRADDLPNFSTFTDETTPCKINPLGAKGVGELGTVGGTPTVMNAVIDALRPLGVT
                     QIEMPATPEKVWRAISQAKAA"
BASE COUNT         1186 a         1717 c         1659 g         1167 t
ORIGIN      
        1 cggtgctcca gtaatcggcc atgcaatcgc gcggaggcgt tcgggtaaca tgtgtccgtc
       61 ccagtatagc ttccgaaaca tcacatcgtg gcagaacaaa ttgattgtgc cgtgattggc
      121 gcgggcgtgg ttggtcttgc catcgcgcgc gaactcgcca tggccggacg cgaagtcatc
      181 gtgcttgaag ccgccgacgc cattggcacc cataccagct cacgcaattc cgaagtcatc
      241 catgccggtc tgtattaccc gaaaggctcg cttaaagcgc gcttttgcgt gaccggaaag
      301 ggtctgctct acgagtactg ctccgtgcac ggcgtgcccc atagcaatct cggaaaaata
      361 gtggtcgcag tgacggcgga tgaagtcact atcttgaaga gctacgtcga gaaggcgcaa
      421 cagaatggcg tcaccgatct ccgctggctg tcagtcgaag ccttgcgcga actcgaaccc
      481 gccgtggaat gtgtggccgg attccattcg ccttctacag gaattatcga cagccacgcc
      541 ctcatgctcg cctatcaggg cgatgcagaa agctgcggcg cgtctgtcgt gttcaaaagt
      601 cgcgtagagt ctggcgaagt cactggtgag ggcatcgttc tgaacgtatc cggagaagag
      661 tcgatgagca tcgcctgcca gagcgtggtg aattcagcag gacttttcgc gcaggacgtt
      721 gcccgctccg tccagggaat accgaaatcc agcataccac cgcagtattt cgccaaagcc
      781 cactattaca cgctgtccgg caaggctccc ttccggcgtc tggtttatcc ggtcgcgagt
      841 cacgcgcatc tgggcgtaca cgttacggtg gacatggccg gacagatccg cttcggcccc
      901 gatgtgtcct gggttaacgg cgtcgattac acattcgatg tgtcgcgcga accgctcttc
      961 tacgaggcca tccgcaagta ctatccggcg ctgaagcagg gccaactgca accggggtac
     1021 acgggtatcc ggcccaaggt ttccggtcca aaggagccgg cggccgattt catcattcag
     1081 gggccaaagc atcatggagt cgcgggactg gtcaatctat acggcatcga atcgcccggg
     1141 ctcaccgcat cacttgcaat cgcgcagcac gtcaagcaat tgctgcaggg ttaaactggc
     1201 acaccaaggc ggagaaaagt tatgggagca atggaccaac tggagcccat tgaaaaggcg
     1261 ggtgtggacg aactgcgcac gctgcaactt gagcgccttc gctggagcct gctgcacgcc
     1321 tacgaaaatg tgtcccacta ccgcaaggcg ttcgatgcgg caggcgctca tccatccgat
     1381 ctgaaacagc tttccgatct cgaaagattt ccgtttacga ccaagacgga gttgcgcgag
     1441 aactacccgt ttggcatgtt cgcggtgccg atgaatgaca tcgttcgcgt acacgcctcc
     1501 agcggcacca ccggcaagcc tacggtggtc ggttacacca aaaaagacat cgggatgtgg
     1561 gcggccgtga tggcgcgctc gctgcgcgcg gcaggggtca aatccagcga catcgttcat
     1621 atcgcgtatg gctatggact attcaccggc ggacttggcg cgcactatgg agcggaggcc
     1681 cttggcgcca cggtgattcc gtcgggtggc ggcatgacgg aacgccaggt caggctgatt
     1741 tgcgatttca agccggacgt catcatgtgc acgccctcgt atcttctggt gattgcggat
     1801 gagttcgcgc gccagggact ggacccggca caatcctcgc caaggatcgg catttttggc
     1861 gcggaaccgt ggtccgaaag catgcaggcc gaactcgagc gctgcttcgg gatcgtggca
     1921 ctggacttgt atggcctgtc ggaagtcatc ggtccgggcg ttgcagcaga atgtattgag
     1981 tcgcgcgatg ggctcaccat ctgggaagac catttctatc ctgaaatcat cgacccgcgc
     2041 actggcgcag tacttccgga aggcgcaacc ggcgaactcg tattcacctc gctgaccaaa
     2101 atcggcatgc cagtgattcg ctatcgcacc cgcgatctca cgcgtttgtt gccaggaacc
     2161 gcacgtacca tgcggcgtat ggcgcgcgtc accggtcgca gcgatgacat gctaattatc
     2221 cgcggcgtca acgtctttcc gtcccaggtc gaggaaatcc tcgtgcagca gtcagcgcta
     2281 tcgccgcact atgtgctcga agtcacccgc gacggacata tggatgagct ggacgtcctg
     2341 gtggagcgcc gtgcggatat cccggggccg cttgccgcca ccgcaaggcg ctctatcgaa
     2401 caggaagtgg agcgcatgat caagggcctg attggtgtca ccacgaaggt acgtgtggcc
     2461 gagccgcaga ccctgccccg ctcccagggc aaggccgcac gcgtcatcga caagcgaccc
     2521 cggaattgaa ggaaatcaca tgatcacatc ttccggactg acgccagccg aaccattgct
     2581 caaggtaaac ctggagaacg aagccgtcgc aggctggctt gcttcatcac cgcgcgatgt
     2641 cggtgacttt tccggagact gcatacgcta ttcgcgattc tggcaggcca gcgcaacctt
     2701 gcttggccga ttgccggcca aaccggaacg caacgcgcag caggcagaag cggccaagtc
     2761 gattcatggt ctggcccgcg aagcacgcgc gcgttttctg tctgcgcact ccggcgcgct
     2821 gtacgaccgc ctgacgcgca gcaggacgcg ctttgttcgt gtagaacaac ttgtctacga
     2881 tgcagcggaa ctcgtcccgg gattaacacc cacgcaagcg caagtggatg gcgaactggc
     2941 gctcatgcag cgcgataagg aaggccatga aatcgatcag ggaattttga tttcccatgt
     3001 gcttgccgac caaagcgccg gaacacacct ctgccatgcc atgcttttgc cgcgcccgga
     3061 ggcaatcgag cggcttgcgg aatttcaggc caatggccgg attgatctcg gtgccgcgga
     3121 agtcttccgc aacggaaagg ccgcgtacgt cattcagaaa aacccgcggc atctgaacgc
     3181 cgaagacgac agtaccatcg acgcggcgga aatcgcagtc gatctcgcca ttctcgatcc
     3241 gcaaagccag atcggcgtgc tacgcggaga ctacgtgcag ggtggaaaat atgatggccg
     3301 gcgcatgctc ggcagcggaa tcaatctaac gcatctgtac tacggcaaga ttccctacat
     3361 ctggtatttg cagcgcgacc ttggcatcgt aaacaaattt ttccgcggcg tggcccgcga
     3421 agacgtgata ccggatgaca ttaccggtga gactaacgaa aaaccctgga tcgccgtcgt
     3481 ggagcaattc gcaatcggtg gacattgcca gtatctcctg acaatggatt acgtactggc
     3541 ggcgcgcgga gcgttcatga cgctgccggc acgcaaggag ggcatcattc cgggagcagc
     3601 caacatgcgg atgccgcgct ttactggcga gcgcatcgcg cggcaggcga ttctgtatgg
     3661 gctgcggctc gaatgcgata gtacggaggg ccggctcatg tgcgatgaga ttgtagcgcc
     3721 cgaagaaatg gacgccgcaa tagagcgggt ggtgaaaaat ttcaccggat cgggcatcgt
     3781 cagcgcggca tcaaaccgcc gcgcgtttcg ggtcacccag gagccgctgg acttgtttcg
     3841 ccgttacttc gccacctacg cacgggaaca ggcttactgc cacttcagtc cggcattgat
     3901 cgagaacctg gaaacccact ggaacgccaa aactcgcaag ctttgagcta atcggttcct
     3961 tcgttcgcca ctatccatgg ccgttggctg atcactctgc ctgccgatcg ggtatcatgc
     4021 gcgccgccgc gattcttatt cggacccgag ccacgggctc aagcttagtc cgcgcacaaa
     4081 tagcggcgtc ctttacaaca atcaggagat aacaaagcat gaccgctgca atcatgatta
     4141 ttggaccgct ttccgtcgtc attggctgcc tttactgggt gatgtcgctc ggtaaggcgc
     4201 aagcaacact gaatgcggac ctttggccca agatcagcaa gtaagcgctg acgccaaaag
     4261 caaaagcccc gaagctccgg ggcttttttt tgcgtgtcag cgttggttcc gacctaagcg
     4321 gccttggcct gactgatcgc ccgccatacc ttttcgggcg tagccggcat ttcgatctgt
     4381 gtcacgccaa gtgggcgcaa ggcgtcaatg acagcattca tgactgtcgg agtaccaccc
     4441 accgtgccaa gctcgcctac ccccttcgcg cccagcgggt tgattttgca aggcgtggtc
     4501 tcgtcggtga aggtactgaa attcggcaaa tcgtccgcgc ggggcaaaca gtaatcctgc
     4561 agggagccgg taatgagttg gcccgtctgc ggatcgtaat gcgcgttttc caatagagcc
     4621 tggcccaccg cctgcgcgat gccgccgtgg acctgacccg caacgattag cggattgatt
     4681 acgcgaccca cgtcatccac cgtcgtatag cgcacgatct gcgtaacgcc ggtatcggcg
     4741 tcgatctcca cttcgcagac atggcaactg ttcggccagg agggtccatc gactttttgc
     4801 aaagtcgaaa ttgcaatgcg tttttccggt tgctggcccg ccagctcgaa cagtccgatg
     4861 ccgaggtcgg taccggccac cttgaagctt ccgtcacggt attcgatgtc ctgacccgct
     4921 gcttccagcg cgtcacctgc cagttcacgg cctttttcga tggcttgctt cgaggcggtc
     4981 agcatggccg atccaccaat gtagagggag cggctgccca tgctgccgat accctgcccg
     5041 atgtcgctgt cgccttgaac aatgacgatt ctttcgggat cgatggccag cgtttcggac
     5101 agaatctgca cgtaactggt ctcgatgccc tgccccatgg cctgcatcgc ggtatagacc
     5161 agcacgcgtc catcggcctc gacatgcagg ttcacgcttt cctcatgcac cacaccggtc
     5221 cactcgagga acgtcgacag tgcgcgaccg cgcagcttgc cgcgcttgcg cgattcctcg
     5281 cgccgtgcgg catagccttt ccagtcggcc tgctgcagga ttcgatccag catatgcggg
     5341 aaattgccgc tgtcgaattt ctcccccatc ggtgtcttgt agggcatttg cgagggctgg
     5401 ataaagttgc gtcggcgcaa ttcggccgga tccattttca tttccgccgc cgcctggtcc
     5461 atcagccgct ccagcagata aatcgcttcc ggccgtcccg cgccgcggta ggcgccgacg
     5521 acattagtat tggtcatcac cgccgtgccg cgcaaatgca gtgttggaac gtgatagacg
     5581 ccagtgataa ctttcggccc gactgcgacc acgattacag cacccggtcc actgacatgc
     5641 gctccgatat tgccaacgat gtcgacccgc aagcccagaa tcttgccttc gccgtcgaat
     5701 gccagttcgg catgattggt ctggtcgcg
//
LOCUS       DATMZQ010000394         3359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_310156, whole genome shotgun sequence.
ACCESSION   DATMZQ010000394 DATMZQ010000000
VERSION     DATMZQ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310156"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VN496_12660"
     CDS             complement(<1..329)
                     /locus_tag="VN496_12660"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HXF17854.1"
                     /translation="MSWLIAGLALFFGVHVFSSMRSARGRLIAKLGEGPYKGSYALLS
                     LAGFGLIVAGMAKAPFIELWDPPTWGRYAALWLMPVALVLIAAAYIPGNLKRFTAHPM
                     LWGITLWA"
     gene            complement(354..929)
                     /locus_tag="VN496_12665"
     CDS             complement(354..929)
                     /locus_tag="VN496_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17855.1"
                     /translation="MKPQVLLLIVLGLLIGPGYYAFCEHVSGRSGQSFALTERGNRWP
                     LPDGSVLRLRGGQAYKPLPLELTPEQNNYRLRFTFNVTQPDSSGATNEYQVSLMQGDS
                     GVAERSIKVKGRGNVSVALEPLQIYYPGSYLLVLEEVGTPPLAVSGVGLQVDTGVEKP
                     KMWVAWSGLVLLVFGIGMVLRDVVAQAHKRR"
     gene            complement(1003..1821)
                     /locus_tag="VN496_12670"
     CDS             complement(1003..1821)
                     /locus_tag="VN496_12670"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXF17856.1"
                     /translation="MGNNLDAAQIAKYRKDGYVFPVPALTSGEATEVRTGIEDFERRS
                     GLVAGHVIRNKGHLKLTRLYDLVFHSRVLDAVESILGPDILCWGSSLFVKEGNDPGFI
                     AWHQDSYYWGLEPDDVVSAWIALYPSTVENGAMRVIPGSQTMPSLSHRKSDAQSTNML
                     FTHEELADDVDESKAADITLAQGDMSLHHIKILHGSPPNRSSGRRMGYAIRYVAPHVK
                     QRGDMNSATLVRGADRYGYFAKDPVPARDMDPDVVAFVDRRYGGTPLGATATIR"
     gene            1937..3178
                     /locus_tag="VN496_12675"
     CDS             1937..3178
                     /locus_tag="VN496_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HXF17857.1"
                     /translation="METTILLIFGVVYLGMILGGLPFLSLDRTGIALLGAIALIGAGA
                     INLEEAWQAVHVPTLILLFAFMVVSAQLRMGGFYTWVTHQTGHLPLSPPKLLAALIAI
                     VAALSAVFSNDVVCLAMAPVLIDVCLARRLNPIPFLLALACGANVGSALTLIGNPQNM
                     LIGERLKLSFSGYLLEAAIPVVLGLFVVWGVIVLSSRRAWALAAEEAATDALPAHLEP
                     APRVDRWQTAKGLSVAAVVFILFLFAPVPREVVALTAAGVLMMSRRLHSRKMLGLVDW
                     QLLVLFIGLFVVNHGLEKTGLIHQFVGRMAALGVDLHEPLPLFVASFALSNIVSNVPA
                     VMLLLPVATHELAGPLLALASTLAGNLLIVGSIANIIVVDAAHRRGLTIDWKRHARIG
                     IPVTLATMAISWLYLWLRVLR"
BASE COUNT          603 a         1014 c         1022 g          720 t
ORIGIN      
        1 gcccacagcg tgattcccca tagcatcgga tgcgccgtga accgtttcag attgccggga
       61 atgtaggccg cggcgatcaa taccagtgcc acgggcatga gccagagcgc ggcatagcgt
      121 ccccatgtgg gagggtccca cagctcaatg aatggcgcct tggccatgcc ggcaacgatc
      181 aatccgaaac cggccagtga aagcagggca taggagccct tgtagggacc ttcgccaagt
      241 tttgcgatca gccgcccgcg cgcggagcgc atgctcgaga acacatgcac gccgaaaaac
      301 agcgccagac ctgcaatcag ccaagacatg atttcaccct ccccccattt gtgtcagcgg
      361 cgcttgtgcg cctgcgcgac gacgtcgcgc aacaccatgc cgatgccgaa aacgagcaat
      421 accagtccgc tccatgcgac ccacattttc ggtttttcca cgcccgtatc gacctgcagg
      481 cccacgccgg aaaccgccag cggcggcgta ccgacttctt ccagcacgag cagatagctg
      541 ccgggatagt aaatctgaag cggttccagc gccacgctga cattgccccg gcccttgacc
      601 ttgatgctgc gctcggccac accgctgtcc ccctgcatca ggctgacttg atattcattc
      661 gttgcgccgc tggagtcggg ctgcgtgaca ttgaaggtga agcgcagccg gtaattgttt
      721 tgctcgggtg tgagttccag cggcagcggc ttgtaggcct gtcccccgcg cagcctcaat
      781 actgaaccgt ccggcagtgg ccagcgattg ccgcgctcgg taagcgcaaa actctgcccg
      841 gagcggccgc tgacatgttc gcaaaacgcg taatagccgg ggccgatcag cagccccagc
      901 acgatcagaa gcaggacctg cggtttcacc cggcggcttc tttgcggatt gtcactgaca
      961 tggccaggat tatcggccgt actttgttag acgtcgatca aatcacctga tggtggctgt
     1021 agcgcccaac ggcgtgccgc cgtaacgtcg atcgacgaac gcgacaacat cggggtccat
     1081 gtcgcgcgcg ggcaccggat cctttgcaaa ataaccatag cggtctgcgc cacgcaccag
     1141 ggttgcggaa ttcatgtcgc cgcgctgttt cacatggggc gcgacatagc ggatcgcata
     1201 gcccatgcga cggccgctgg accggttggg cggcgagccg tgcagaatct tgatgtggtg
     1261 cagcgacatg tctccttgcg cgagcgtgat gtccgccgct ttggattcgt caacgtcgtc
     1321 ggcaagttct tcgtgcgtga acaacatatt cgtgctttgc gcgtcggact ttcggtgcga
     1381 cagggaaggc atggtttggc tgccgggaat gacgcgcatc gcgccattct ccacggtgct
     1441 gggatacagg gcgatccagg cggagactac gtcgtccggt tccaaccccc aatagtacga
     1501 atcctggtgc caggcgatga aaccgggatc attgccttcc ttgacgaaaa gactcgaccc
     1561 ccagcacaga atgtccgggc cgaggatgga ttccacggcg tcgagtaccc tggaatggaa
     1621 gaccaggtcg tagaggcggg tgagtttgag gtggcccttg ttgcggatga catgcccggc
     1681 aaccagtccg ctgcggcgct cgaaatcctc gatgccggtg cgcacctcgg tggcctcgcc
     1741 ggaggtcagc gcgggtacgg ggaaaacgta gccatccttg cgatacttcg cgatctgcgc
     1801 cgcgtccaga ttattaccca cacgtcctcc ttttttcaaa tgaacgcggc attgtcgcat
     1861 gcgcgagcgt gacgatgccc gcaagcccgt tcggatgacg tagactgccg cgggcttcga
     1921 aactgacggc gacaagatgg aaaccacgat tctgctgatc ttcggcgtgg tctacctggg
     1981 catgatcctc ggcgggctgc cttttctgtc gctcgaccga accggcatag cgttgctcgg
     2041 tgccattgcg ttgatcggag ccggggcgat caacctggaa gaagcgtggc aggcggtcca
     2101 tgttcccacg ctgatcctgc tgttcgcctt catggtcgtg tccgcacaac tgcgcatggg
     2161 cggcttctat acctgggtga cgcaccagac cgggcatctt cctttgtcgc cgcccaaact
     2221 gctcgcggcg ctgatcgcca tcgtcgccgc actgtcggcg gtgttcagca acgatgtcgt
     2281 ctgtctggcc atggctccgg tgctgatcga tgtttgcctc gcgcggcgac tgaatcccat
     2341 tcccttcctt ttggcgctgg cgtgcggtgc caacgttggg tcggcgctga cgctaatcgg
     2401 caatccgcag aacatgctga tcggtgagcg cctgaaactc tccttttccg gctatttgct
     2461 ggaagcggcg ataccggtag tgctgggact gttcgtggtg tggggagtga ttgtcctgag
     2521 cagtcgcagg gcctgggcgt tggctgcgga ggaagcagcg accgatgcgc ttcccgccca
     2581 tctcgaaccg gctccgcggg tcgatcgatg gcaaacggcg aaaggcctct cggttgcagc
     2641 ggtggtgttc atactgtttc tcttcgctcc cgtgccgcgc gaggttgtcg ccctcaccgc
     2701 ggcgggtgtg ctgatgatga gccgccgtct tcattccagg aaaatgctcg gtctggtgga
     2761 ctggcaactg ctggtcctat ttatcgggtt gttcgtcgtc aaccatgggc tggaaaaaac
     2821 cggcctgatc catcaattcg tcggccgcat ggcagcactc ggcgtggatt tgcacgaacc
     2881 tttgccgctt ttcgttgcaa gtttcgccct gtcgaacatc gtgtccaacg ttccagccgt
     2941 catgttgttg ctgccggtcg cgacccacga attggccggg ccgctgctgg cgctggcgag
     3001 caccttggcc ggaaacttgc tcatcgttgg tagcatcgcc aatatcatcg tggtggacgc
     3061 ggcgcacaga aggggattga ccatcgactg gaaacgtcat gcccggatcg gcattccggt
     3121 gactttggcc acgatggcga tttcctggct ctatctgtgg ttacgggtac tgcgatgaag
     3181 gctcaacgta ttgggttccc ggacgttaaa gctgccatga agtccgcttt cctttcgctg
     3241 ctgttcctgt tgccgtccct cgctcttgcc gcggatgtgc aatccgtgga attgcaccac
     3301 gagctcgtat tgcctccagg cgtcaaagtc gtcgcgttga cgctggatgc ctgcggtgg
//
LOCUS       DATMZQ010000395         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4645407, whole genome shotgun sequence.
ACCESSION   DATMZQ010000395 DATMZQ010000000
VERSION     DATMZQ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4645407"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..76
                     /locus_tag="VN496_12680"
     tRNA            1..76
                     /locus_tag="VN496_12680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="VN496_12685"
     tRNA            174..247
                     /locus_tag="VN496_12685"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Cys,seq:gca)
     gene            364..454
                     /locus_tag="VN496_12690"
     tRNA            364..454
                     /locus_tag="VN496_12690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:398..400,aa:Leu,seq:taa)
     gene            985..1914
                     /locus_tag="VN496_12695"
     CDS             985..1914
                     /locus_tag="VN496_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXF17858.1"
                     /translation="MKNIVSFCIASLLLISSGADALAQITERRVDIYSEGIRLSGTVF
                     HLQSLDGKKLPAVILSHGWGGTASMLRPQAEQFADAGYFVLAFDYRGWGDSDSRWVRD
                     EAAAAGSGDRRELREVVHPLDQATDLLNAVHWAMGESMVDTNRVGLWGTSFSGGLVAY
                     AAARDPRIKAIVSQVAWFGTPRNQMPPDVLSKAESDATRRARGEIGYPTPGVQEVGNL
                     RGGPARESFLLYAPIDDIPAIKQCAILIIDAEKEELFDLRKQGELAFQRATGPKKRVV
                     IPGVSHYDIYGKSREQATMLAIEWLDAHLKSAM"
     gene            complement(1945..2238)
                     /locus_tag="VN496_12700"
     CDS             complement(1945..2238)
                     /locus_tag="VN496_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17859.1"
                     /translation="MERRKKVVQKRRGMWEAIRALLKSDNIEAGNKVLLGIYKKGWAA
                     NQKGQHRFTNPYSSNIPYRDEWNRGWMECSRIIKRRSTDAFEGQFPRNYSVLK"
BASE COUNT          704 a          796 c          797 g          683 t
ORIGIN      
        1 gcgggaatag ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgagc
       61 ctcgtttccc gctccaaagc aaaggggaaa acgctcaata gcgttttccc ttttttagtt
      121 ttcggcgact ccgttcgcca ggtgcactac cagacgacta gtgcgcgcac cccggcgggg
      181 tggcagagtg gtcatgcagc ggcctgcaaa gccgtgtacg ccggttcgat tccgaccccc
      241 gcctccaaat cgctaatgac attccgctga atgatggtct ttagtgccgc cagctttgtg
      301 agttgaccag aggcactacc ggcgctcgcg cgaagggaaa cttacgtaaa attcaccgct
      361 tctgcccgga tggtgaaatt ggtagacaca agggacttaa aatccctcgg ccctttaaca
      421 ggccatgccg gttcgacccc ggctccgggc accaatcaat tgctgttgca tcgtttgcac
      481 aacaggatta gcaggctgaa caaacgaaag gtgattatct gcatttgatg agcatgttgc
      541 tgctacggta agccccgtat tgagtcgcgg gggtagaacc ctgcttcgcg tctcccgcga
      601 ctatgtcgta tccgctatca ccgcaaagtt cttccgcctt ggtgtagcaa tttccccaac
      661 tctgcgcagt cccggagcaa ttgatactgt ggcctatgga gccatctggc aaacggatgt
      721 cgtttaccgt agtgcagccg gcaaagccgg ccataatcac caggaaaagt atgcgcattg
      781 tgatgtgaat tgccgtcctt cattctttgc catgtcaaac tctcccacag ttagcacgac
      841 aatagtgaca atccacaatt gtccgcacta tataaccccc aattgccatg tagagttgca
      901 gtctatcgtg tcctattgat ttcccgatct gaaatcctcg agccagttag agccacgctg
      961 gaatacacac aggagcctgc aaggatgaag aacatcgtat cgttttgcat cgcatcgctg
     1021 ctgttgatct cgagcggcgc agacgcgctt gcgcaaatca cggaacgcag agttgacatc
     1081 tacagcgaag gcattcgctt gagcggcacg gttttccatc ttcagtcact cgatggcaaa
     1141 aaactgcccg ccgtcatctt gtcccacggc tggggtggca cggcatcgat gttacggcca
     1201 caggcagagc aattcgcgga cgctggctac ttcgtactgg cgttcgacta tcggggctgg
     1261 ggagatagcg attcacgctg ggtacgggac gaagccgcgg cagccggatc gggcgacaga
     1321 agagaacttc gcgaagtggt gcatccgctg gatcaggcca ccgatctatt gaatgctgtg
     1381 cactgggcga tgggcgaatc gatggtcgat accaaccgcg tcggcttgtg gggcacaagt
     1441 ttttcgggtg gccttgtcgc atatgcagca gcgcgtgatc cccgcatcaa ggcaatcgtc
     1501 agccaggtag cctggtttgg gacgccgcgt aaccagatgc cgccagatgt cttgtccaaa
     1561 gcggagtcgg atgcgacccg ccgagctcgc ggagagatcg gctacccgac acccggtgtc
     1621 caggaggtcg gcaacttgcg tggcgggccg gcacgggaaa gctttctgct ctacgcaccg
     1681 atcgacgaca ttccagccat caagcaatgt gccattttga tcatcgatgc ggagaaagag
     1741 gaacttttcg acctgcggaa gcagggcgaa ctagccttcc agcgggcgac cggaccaaag
     1801 aagcgcgtgg tgattcctgg cgtcagccac tacgatatct acggaaaatc gcgtgagcag
     1861 gccacgatgc ttgcaatcga atggctggac gcgcacctca agagtgctat gtaaaagcgc
     1921 agtccagcga tcacgacaat cgcattattt cagcacgctg tagttgcgcg gaaattgacc
     1981 ttcgaacgca tctgttgagc gccgcttgat gatcctgcta cattccatcc aaccccggtt
     2041 ccattcgtcc cggtaaggaa tattgcttga atagggattg gtaaatcggt gttggccctt
     2101 ttgatttgca gcccagcctt tcttataaat accaagcaga actttgtttc ccgcttcaat
     2161 attatcgctc ttgagcaagg ccctgattgc ttcccacata cccctgcgct tctgtactac
     2221 ctttttacgc ctttccataa actcctccac gtttgatggg ttttctattt atttgccata
     2281 gagtaaccag gattccaggt aactgccaag gctatcctaa tccgacttgt tcttgcgttc
     2341 ctagtgataa ccgtcggcta ccctcatccg gaacgttgat cccggagagg acgcctggtg
     2401 gaagcattgt ttcggcaatc tacgcaagcg agattgccag agccgaacca ccctggatac
     2461 tgtcaagaga cggcgccgca cacttgtgat accgtgcaac cgcgtccgcg gaatagagaa
     2521 gaaaaaaggg gaggcgggtt aatagctggg gaggcgggtt tctcgtagcc cgttaagcgc
     2581 gttccgcgga cgcaccatcc catatattcc agacaaaaaa aaccgcgatg cagagcaaag
     2641 cggttttagg cccaagccaa aagcgaaggg aggcgacact tttggccgga tgaaaatgaa
     2701 accaatagag gtcgatgtac gggaagatta tttcccttac ttaagtcttt ggatttgcaa
     2761 aatagggcag gattgccatg cttcgcgcga cgctcgatct tttaactaga tcgttttgtc
     2821 acggaaggag taaggatagc taccgcaagt ccattctgca acgagcttta caaaaaatcg
     2881 tcacactgcg catggtgctc gctgacggaa taccaaaaat aatctaaaag aaacaaattg
     2941 gaatctcgga cagtgctata tgtgcttgag gtttgggttg
//
LOCUS       DATMZQ010000396         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3418340, whole genome shotgun sequence.
ACCESSION   DATMZQ010000396 DATMZQ010000000
VERSION     DATMZQ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418340"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..956)
                     /locus_tag="VN496_12705"
     CDS             complement(171..956)
                     /locus_tag="VN496_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXF17860.1"
                     /translation="MLKLYEHPLSAYAMKVKIALNEKNIEYQTAIPDGMAVGKAGGEF
                     VDANPRAEVPALIDDNFSVFDSTVILEYIEDKWPDPPLLPKSPAERARVRMIEDVMDT
                     QYEQNNWGTFEVLRYQRASGTLADTLTAYAKNNIEQLQGWLERQLGNRHWMNGEAFGW
                     GDVSVIPYLNRSAGYGYAPRKGSRLLAWFERVNQRESVAKVRAQMQAVLDKMPDLGQM
                     LKSGQVVRQYRDHRLEWMIAAGGISVVLEGMQKGTIRFSRNVA"
     gene            complement(1012..1986)
                     /locus_tag="VN496_12710"
     CDS             complement(1012..1986)
                     /locus_tag="VN496_12710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HXF17861.1"
                     /translation="MKAVLCREWGLPGKLVVEDVPSPKPATGQVVVSVKACGVNFPDT
                     LIIQGKYQFKPEMPFSPGGEVAGIVKETGEGVTRVKVGDRVIAFNTWGGFAEEMVVDA
                     DRTIAMPSSMEFVPASAFVLTYGTSYHALKDRADIKAGETLLVLGAAGGVGLAAIQLG
                     KAMGARVIAAASSDVKLNVCRDNGADEVINYGSEDLRARVKTLTAGKGVDVIYDPVGG
                     PYSEPALRDMAWKGRFLVVGFAAGDIPKVPLNLTLLKGCSIVGVFWGAFTRNEPERNR
                     ENNWELMAMYAAEKIKPHIHATYPLERAAEALNEVLNKRVTGKVVLVT"
BASE COUNT          420 a          682 c          533 g          511 t
ORIGIN      
        1 cgggttgggc ctttattgaa ggaactcatg tacgcgtgat cgcactgaaa agaaattcgt
       61 atagatttgg acaagtcctc cagcacttgg cttccatcag aacgcctcgt tcaaccgaca
      121 gacactcgcg gaccgaaatt atgcgatctg cgcggttctc tggtggtcga ctaagctacg
      181 ttgcggctga aacggatcgt ccccttttgc attccttcca gcacaaccga gatgccgccg
      241 gccgcgatca tccattccag ccggtggtcg cgatactggc gcactacttg tccgcttttc
      301 agcatttggc ccagatcggg catcttgtcg agcaccgcct gcatctgcgc tctgactttg
      361 gccacgcttt cgcgctgatt cacgcgctca aaccaggcaa gcagccggga tcccttgcgc
      421 ggcgcataac cgtacccagc cgaacgattc aaatagggaa tgactgaaac gtctccccaa
      481 ccgaaagcct cgccattcat ccagtgtcga ttgccgagct ggcgctcgag ccagccctgt
      541 aattgttcga tattgttttt cgcatacgca gtcaatgtgt ctgccagcgt cccgctcgca
      601 cgctggtaac gaagcacttc gaacgttccc caattgttct gctcgtattg tgtatccatg
      661 acatcttcga tcatgcggac ccgtgcgcgt tccgccgggg atttgggcaa caggggcgga
      721 tcgggccatt tgtcttctat gtactccagt atcacggtgg aatcgaacac gctgaagttg
      781 tcatcgatca gcgcagggac ttctgcgcgt ggattcgcat ccacgaattc gccgccggcc
      841 tttcccaccg ccattccgtc tggaatcgca gtttggtatt cgatattttt ttcgttcaac
      901 gcaatcttga ccttcatcgc gtacgccgac agcggatgtt cgtaaagctt cagcatttcc
      961 ctctccttaa cattcgtgaa gcgtggcgcg cccgacgcac cttgacgaca tttatgtgac
     1021 cagaacgacc ttaccggtta cacgcttgtt cagcacttca ttgagcgctt cggctgctcg
     1081 ctccagcgga taagtggcgt ggatgtgcgg ttttattttt tccgcagcgt acatggccat
     1141 caattcccag ttgttttcgc gattgcgctc gggctcattc cgggtaaatg cgccccagaa
     1201 cacaccgacg atcgagcagc ccttcaacaa cgtaagattt agcgggactt tgggaatgtc
     1261 gcccgccgca aaaccgacta ccaggaagcg gcccttccac gccatgtccc gcagcgccgg
     1321 ctctgaatag ggcccgccca ccggatcata gatgacatcg acgcctttgc ctgccgtcag
     1381 cgtttttacc cgcgcgcgca aatcttcaga tccataattg atgacctcat ccgcgccatt
     1441 atccctgcac acattcaatt tcacgtcgct ggaggcagcc gcaatgacgc gcgcccccat
     1501 cgccttgccg agttgaatcg cagccagccc gacaccgccc gccgccccaa gcacgagcag
     1561 ggtttcgccc gccttgatgt ccgcccggtc cttcaatgcg tgatacgagg tcccgtaggt
     1621 cagcacgaag gccgatgccg gaacgaactc catgcttgac ggcattgcaa tggtgcgatc
     1681 cgcatcgacc accatctctt ctgcgaagcc gccccaggta ttgaaagcga tgacccgatc
     1741 gccgaccttg actcgcgtta cgccctcgcc cgtttccttg acgatgccgg ccacttctcc
     1801 accgggagaa aaaggcatct cgggtttgaa ctgatatttg ccctggatga tcagcgtatc
     1861 cggaaaattt acgccgcatg ctttcacgct gacaacgacc tgaccagttg ccggctttgg
     1921 cgaaggcaca tcctcgacaa ccagcttccc gggcaatccc cattccctgc atagaacagc
     1981 tttcatggtt ctccagatcg gtgcagtgcg gtttaggctt tgattccgcc tttgatcatt
     2041 ttttcgacct gctgccagcc agcctcggcc atcggccgcg cgcgcttgcc ttgttccagt
     2101 gcgtgtgcgc tggcagccgt tccatctttt accctgccca tgatgc
//
LOCUS       DATMZQ010000397         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3074918, whole genome shotgun sequence.
ACCESSION   DATMZQ010000397 DATMZQ010000000
VERSION     DATMZQ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074918"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VN496_12715"
     CDS             <1..258
                     /locus_tag="VN496_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXF17862.1"
                     /translation="SDADIILVDDVLFTGRTIRAAMNELFDYGRPKSIRLAALIDRGG
                     RQLPIAAQFVGDEYAVAEGEQIELTKNEKDELSLVLNRARA"
     gene            255..1211
                     /locus_tag="VN496_12720"
     CDS             255..1211
                     /locus_tag="VN496_12720"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXF17863.1"
                     /translation="MTSYNLQLNAQGELQHLLTIEGLPQEIIVRILDTAHSFLSVGER
                     EIKKVPLLRGKAVFNLFFEASTRTRTTFEIAAKRLSADVINLNVGTSSQSKGETLLDT
                     VANLSAMHADMFVVRHSQSGAAHLIARHVAPYIHVINAGDGRHAHPTQGLLDMFTIRY
                     YKKEFTSLRVAIVGDVLHSRVARSQIHALATLGVPEVRVIGPRTLLPSNVSDLGVQVH
                     HDMESGLKDVDVIIMLRLQNERMNGALLPSAQEFFKYYGLTPAKLAHAKPDAIVMHPG
                     PMNRGIEIESSVADGQQSVILPQVTFGIAVRMAVMSILAGNP"
     gene            1208..2482
                     /locus_tag="VN496_12725"
     CDS             1208..2482
                     /locus_tag="VN496_12725"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXF17864.1"
                     /translation="MKIQIKGGRVIDPGTGVDRTQDVFIAAGKVVAIGEGPSGFQANR
                     VIDASGMVVCPGLVDLSARLREPGYEYKATLESEMAAAVAGGVTSLVCPPDTDPPLDE
                     PGLVEMLKHRARNLNQARVYPLGALTQGLKGERITEMAELLDAGCIAFSQADVPLGDN
                     EVLLRAMQYASTFGYQVWLRPRDAALGRNGVAHDGQVATRLGLAPIPSIAETVALSTI
                     LLLVRQTGAKVHLCRLSTADGIDMVRQAKSLGIKVSCDVSANHVHLSEMDIGYFDSNC
                     HLVPPLRSMRDRDAVRRGVLDGTVDAICSDHTPVDEDAKQLPFGESEPGATGLELLLA
                     LTLKWADEAKLSLASALTKVTSEPARILGIEAGTIIAGARADLCVFDPQARWTVQPAI
                     LKSQGKNTPFTGYEMRGKVRFTLVEGNVVFES"
     gene            2583..>2801
                     /locus_tag="VN496_12730"
     CDS             2583..>2801
                     /locus_tag="VN496_12730"
                     /inference="COORDINATES: protein motif:HMM:NF040252.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HXF17865.1"
                     /translation="MNPLLDFSGLPRFAEFKAEAVTPAIDQLLAECRTLVGLLSNQDT
                     EPDWEGFVEPLDDANERLRRAWGQVSHMN"
BASE COUNT          625 a          747 c          821 g          608 t
ORIGIN      
        1 tccgatgccg atatcatcct ggtggacgat gttttgttta ccggacgtac catacgcgcg
       61 gcgatgaatg aattgtttga ctacggcaga cccaagagca tacgactggc cgccttgatc
      121 gatcgcggag gacgccaact acccatcgcg gcacagtttg tgggcgatga atatgctgtg
      181 gctgaaggcg agcagatcga gttgacgaag aacgaaaagg acgagcttag tttggtgctg
      241 aaccgggccc gggcatgacg agctacaacc tgcagttgaa cgcacagggc gaactccagc
      301 atctgctcac catcgaaggc ttgccgcagg aaatcatcgt acgcattctt gatactgcgc
      361 attccttcct ctcggtcggg gaacgtgaaa tcaaaaaggt tcccttattg cgcggcaagg
      421 cggtgttcaa tcttttcttc gaggcatcga ccagaacgcg caccactttt gaaatcgccg
      481 caaagcgtct ttcggcagac gtgatcaatc tgaacgttgg tacatcttcc caaagcaagg
      541 gcgagacact attggatacc gtggcaaacc tgtctgcaat gcatgccgac atgtttgtcg
      601 tgaggcattc gcagagcggc gctgcacatc tcatcgcaag acacgtggcg ccttatattc
      661 atgtgattaa tgccggcgac ggtcgccatg cgcatcctac gcagggcttg ctggatatgt
      721 tcaccatccg ttattacaag aaggaattta catccttgcg agtggctatc gttggcgatg
      781 tgctgcactc aagggtagcg cgatcgcaga ttcacgcgct ggctacgctc ggtgtaccgg
      841 aagtgcgagt gatcggacca cgtaccttgt tgccttccaa cgtctcggat ttgggggtgc
      901 aggtccacca tgacatggaa agtggattga aggacgtcga tgtcattatc atgctgcgcc
      961 tgcagaacga acgcatgaac ggtgctttgc tgccgtccgc gcaagaattt ttcaagtact
     1021 acggactgac tccagccaag ctcgcgcacg cgaaaccgga tgcaatcgtg atgcacccgg
     1081 gtccgatgaa ccgcggtatc gaaatcgaat cgagcgtggc agacggacag cagtcggtaa
     1141 ttcttccgca agttactttt ggcatcgcgg tacgcatggc ggtcatgtcc attctggcgg
     1201 gaaatccgtg aagatccaga tcaaaggcgg ccgggtcatc gatccgggca ctggggtcga
     1261 tcgcacacag gacgtcttta tcgcagctgg gaaagtggtg gcaattgggg aagggccgag
     1321 cggatttcag gctaaccgcg tcatcgatgc aagcggaatg gtagtttgcc ccgggcttgt
     1381 ggatctctcc gcgagattgc gagaaccggg ctacgagtac aaggcaaccc ttgaaagcga
     1441 gatggcagca gcggtggccg gcggtgtaac ttcgctggtc tgtccccccg acacggatcc
     1501 tcccctggat gagccgggac tggtcgagat gctcaagcat cgggcccgca atctgaatca
     1561 ggcccgcgtg tatccgcttg gcgcattgac gcaaggtctc aagggcgaac gcatcactga
     1621 aatggcagaa ttgctcgatg ccggctgcat tgccttttcg caagccgatg tgccgctcgg
     1681 cgataatgaa gtattgctgc gcgccatgca gtatgcctct acttttggct atcaagtctg
     1741 gctgcgaccg cgcgatgccg cgctcggcag gaatggtgtc gcgcacgacg gccaggtagc
     1801 gacccggctc ggacttgcac cgataccatc catagcagag acggtggcgc tatccacgat
     1861 tcttttgttg gtgcgccaga ccggcgccaa agtgcattta tgcaggctgt cgacggctga
     1921 tggaatcgat atggtccgcc aggcgaagtc cctcggcatc aaagtatcat gtgacgtttc
     1981 ggcaaatcat gtgcacctga gcgaaatgga tatcggctat ttcgattcaa attgccatct
     2041 ggttcctcca ctgcgttcga tgcgcgatcg cgacgccgtg cgtcgcggag tgctcgatgg
     2101 aacggtggat gccatatgct cggaccacac gccggtggac gaagacgcca agcaattgcc
     2161 attcggcgaa tccgaacctg gagcgactgg ccttgaacta ttgctcgcac tgactttgaa
     2221 atgggctgat gaagccaagc tgtctttggc atccgcgctg acaaaagtca cgagcgaacc
     2281 tgcccggata ctgggcatcg aagccggaac aatcattgca ggcgcgcgag cagatttgtg
     2341 tgtctttgat ccgcaagctc gctggacggt ccagccggct atcctgaaga gccaaggaaa
     2401 aaatacgccc tttaccggat atgagatgcg cggcaaggtt agattcacgc tcgtggaagg
     2461 caacgttgtg tttgaaagct gaacagcgtg ggccccgaca ttttggtaaa tggcggaagt
     2521 ggttgaaatt caaattgtcg aacaccagtt aaggctttgt cgcgcacgct cctcttgcca
     2581 acatgaatcc attactcgat ttttccggtc tcccgcgctt cgcggaattc aaagccgaag
     2641 cggtgacgcc agccatcgac cagctgctgg ctgaatgcag aaccctggtc gggcttctga
     2701 gtaaccagga caccgagcct gactgggagg ggttcgtcga accactggac gatgcgaacg
     2761 agcgtttacg ccgtgcctgg ggccaggtgt ctcacatgaa t
//
LOCUS       DATMZQ010000398         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4991569, whole genome shotgun sequence.
ACCESSION   DATMZQ010000398 DATMZQ010000000
VERSION     DATMZQ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4991569"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VN496_12735"
     CDS             <1..469
                     /locus_tag="VN496_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF17866.1"
                     /translation="LDRGAQVLMSGLDYERAVLAGGPLGIMQACMDIVMPYVHQREQF
                     GQPIGEFQLMQGKIADMYTTLNACKAYVYAVAKACDRGQTARKDAAGAILYAAEKATW
                     MAGEAIQALGGNGYINEYATGRLWRDAKLYEIGAGTSEIRRMLIGRELFRETS"
     gene            477..2243
                     /gene="aceK"
                     /locus_tag="VN496_12740"
     CDS             477..2243
                     /gene="aceK"
                     /locus_tag="VN496_12740"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HXF17867.1"
                     /translation="MTTIAADTHEEVAFLIAQGTLDGFNRHYRLFRETSRYAKSLFEG
                     GQWAEIQRISRERIQFYDRRITETVQYLQRRFHTDQLPDAIWQQVKQHYIGLLTNHKQ
                     PELAESFFNSVSCRILHRNYYHNDFLFVRPVLSTEHINSDPPTYRSYYPARDGVRATI
                     AQVLRDFGLRNPFRDLNRDSRWLLRAVREHLGRPILLEANHQIQVLTSLFFRNKGTYA
                     VGKIVNGSHEVPFAIPILRNGDGSLYLDTILLEAEHLAILFGFSRAYFMVDMEVPSGY
                     VQFLRSLLPNKPAAEIYTVLGLQKHGKTLFYRDFLHHLKHSSDCFVIAPGIRGLVMLV
                     FTLPSYPYVFKVIKDVISPPKEVTRQQVKNKYLLVKYHDRVGRMADTLEYTNVAFPAD
                     RFAPELVAELRRLAPSMIEEEGDTLIIKHVYIERRMIPLNMYVDRTSGEQLAHAVREY
                     GDAIRQLAAANIFPGDMLFKNFGVTRFGRVVFYDYDEIAYMTDCNFRHMPQARTPEDE
                     IAAEPWYPVGADDIFPEEFAAFLLGDSRVREVFMEHHRELLDADFWTATQRRIAGGHV
                     EDVFPYPETLRFRRRFAPGAMP"
     gene            2259..>3417
                     /locus_tag="VN496_12745"
     CDS             2259..>3417
                     /locus_tag="VN496_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXF17868.1"
                     /translation="MKTDPIVIIAAARTPMGALQGDLKDFAAPQLGAAAVRAAVERSG
                     VNAEQVQEVIMGCVLPAGQGQAPARQASLGAGLPLTAGCTTVNKMCGSGMKAAMFAHD
                     LLSAGTNDIMVAGGMESMSNAPYLLPKARTGYRMGHQQVIDHMFLDGLEDAYDKGRLM
                     GSFAEDCATKFGFTRDQQDGFAIASLERAQKANRDGSFAWETVPIAVKAGKDERFIEH
                     DEQPFKANFDKIPLLKPAFRKDGTVTAANSSSISDGAAALVMMRRSTADKLALKPLAM
                     VVGHATHAQESALFTTAPVGAMRKLFDRTGWTPDQVDLYEINEAFAVVTMAAMREHKL
                     PHEKVNVHGGACALGHPIGASGARIVVTLIGALRKRGLRRGVASLCIGGGEA"
BASE COUNT          740 a          986 c          999 g          692 t
ORIGIN      
        1 gcttgaccgc ggagcccaag tgctgatgag cggactggac tacgagcgcg cggtgctggc
       61 cgggggaccg ctaggaatta tgcaggcctg tatggacatc gttatgccct acgtgcatca
      121 gcgtgaacaa ttcggacagc caatcggtga gtttcagctc atgcaaggca agatcgccga
      181 tatgtacacc acgctgaatg cctgcaaggc ctatgtctat gcagtggcca aggcgtgcga
      241 tcgcggccaa accgcccgca aggacgcggc gggcgccatt ctgtacgcag ccgaaaaagc
      301 cacgtggatg gcgggcgagg cgatccaggc actcgggggc aacggctata tcaacgaata
      361 tgccaccgga cggctgtggc gcgacgcaaa actctacgaa atcggcgccg ggacttccga
      421 gatcaggcgc atgctgattg gccgagaact ttttcgggaa accagttgag cgtttgatga
      481 ctacgattgc agccgatacg cacgaggaag ttgccttcct gatcgcacaa gggacgcttg
      541 acggtttcaa tcgccattac cgcttattcc gtgaaaccag tcgctatgcg aaatcgctgt
      601 tcgaaggcgg acaatgggcc gaaatccagc gcatttcgcg cgagcgcatc cagttttacg
      661 accggcgcat aaccgagact gttcaatacc tgcaacggcg cttccacacc gaccagcttc
      721 cggacgcgat ctggcagcaa gtaaaacagc actacatcgg cctgctgact aaccacaagc
      781 aacccgagct agccgagagt tttttcaatt cggtgagttg ccgcattctc caccggaatt
      841 attatcacaa cgattttttg tttgtacgtc ctgtcctgtc gacagaacat atcaattcgg
      901 acccgcccac ttaccgaagc tattatccag cccgggatgg cgtgcgcgcc accattgcgc
      961 aagtgcttag agatttcggc ttgcgcaatc cattccgcga cctaaaccgc gattcccgct
     1021 ggctgctgcg ggccgtgcgc gaacatcttg gcagacctat tctgcttgag gcaaatcacc
     1081 agatccaggt gctgacgtcg ctgttctttc gtaacaaggg cacttacgcc gtcggcaaga
     1141 ttgtcaatgg cagccacgaa gtccccttcg cgatccccat cctgcgcaat ggtgatggca
     1201 gcctgtatct cgacaccatc ctgctggagg cggagcatct ggccatcctg ttcggcttca
     1261 gccgcgcata ctttatggtc gatatggaag ttccgtcggg ctatgtccag ttcctcagga
     1321 gcttgctgcc gaacaaaccg gcggcggaga tctacaccgt actgggcctg cagaaacacg
     1381 gtaaaacgct tttctatcgc gattttttgc accacctgaa acattccagc gactgctttg
     1441 taattgcgcc cggcatacgc ggtctcgtta tgctggtttt caccctcccg tcctacccct
     1501 atgtgttcaa ggtcatcaag gacgtcattt cgccgccgaa ggaggtcacg cgccagcaag
     1561 tcaaaaacaa gtacctgctg gtcaagtatc acgatcgcgt tgggcggatg gcggatacgc
     1621 tcgaatatac aaacgtggca tttccggccg accgttttgc gccggaactt gtcgcggagt
     1681 tgcgcagact ggcgccctcg atgattgaag aggaaggcga taccctgatc atcaaacacg
     1741 tttatatcga gcggcgcatg attccgctga acatgtatgt cgatcgtacg agcggcgagc
     1801 agctcgcgca cgccgtgcgc gagtacggcg atgccatccg gcagcttgcg gcagccaata
     1861 tttttccggg cgacatgctg ttcaaaaatt ttggcgtgac tcgcttcggt cgcgtggtgt
     1921 tctatgatta cgacgagatc gcctacatga ccgactgcaa tttccgacac atgccgcagg
     1981 cgcgcactcc cgaggacgag atcgcagccg agccgtggta cccggttgga gctgacgata
     2041 tttttccgga agaattcgct gcattcctgc tgggagattc aagagtacgc gaggtattta
     2101 tggaacatca tcgcgagctg ctcgacgcag acttctggac ggccacgcaa cgacgaatcg
     2161 ccggcgggca tgtcgaagac gtattccctt accccgaaac gctgcgcttc cggcggcgtt
     2221 tcgctcccgg cgccatgccg taacgaggag actgaatcat gaaaaccgat ccgatcgtaa
     2281 tcattgcggc tgcgcgcacc ccgatgggag cgctgcaagg tgacctcaag gatttcgccg
     2341 ccccgcagct tggtgcagcg gctgtgcgtg cagccgttga gcgttccgga gtaaacgccg
     2401 agcaagttca ggaagtcatc atggggtgcg tgcttcctgc ggggcaagga caggccccgg
     2461 cgcgccaggc ctcgctcgga gccggactcc cgctgaccgc gggttgcacc acagtcaaca
     2521 agatgtgcgg atccggaatg aaggcggcca tgtttgcgca cgatcttctc agcgcaggca
     2581 ccaatgacat catggtcgcc gggggcatgg aaagcatgag taacgcgcca tatctgctac
     2641 cgaaggcgcg caccggctac cgtatgggac accagcaagt catcgatcat atgtttctgg
     2701 acgggctgga ggatgcctac gacaaaggtc gcctgatggg cagcttcgcc gaggactgcg
     2761 ccacaaaatt cggctttacg cgcgaccaac aggacggttt tgcaattgct tcgctggagc
     2821 gcgcacagaa agcaaacagg gatggctcat tcgcgtggga gaccgtgccg attgcggtta
     2881 aagcgggcaa agacgagcgc ttcatcgagc atgatgagca gcccttcaaa gccaacttcg
     2941 acaagattcc cctgctcaag cccgcgtttc gcaaggatgg tactgtcacg gccgcgaatt
     3001 ccagttccat ttccgatggt gccgcggcac tggtgatgat gcgtcgttcg acggcagaca
     3061 agctcgcgct caagccgctg gcgatggtcg ttggacacgc gacgcatgcg caggaatcgg
     3121 cactgttcac caccgcgccg gtgggcgcga tgcgcaaact gttcgacagg acgggctgga
     3181 cacccgacca ggtggatctc tacgagatca acgaagcctt cgccgtggtc accatggccg
     3241 cgatgaggga acataagttg ccgcatgaga aagtcaatgt gcatggtggc gcttgcgcgc
     3301 tgggacaccc gatcggggca tcgggcgcgc gcattgtagt cacattgatt ggcgcgctga
     3361 gaaagcgtgg tctcagacgc ggcgtcgcgt cactttgcat cggtggcggg gaagcca
//
LOCUS       DATMZQ010000399         8698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3077695, whole genome shotgun sequence.
ACCESSION   DATMZQ010000399 DATMZQ010000000
VERSION     DATMZQ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3077695"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VN496_12750"
     CDS             <1..258
                     /locus_tag="VN496_12750"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17869.1"
                     /translation="ALFAEILLALETVLRRFSTEGFASFSEEWNALHAYQDKMVRMRM
                     PDKSEIEGRVQNIGDDGALLLHTRAGLRKFYGGEMSLRGIA"
     gene            289..1017
                     /locus_tag="VN496_12755"
     CDS             289..1017
                     /locus_tag="VN496_12755"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXF17870.1"
                     /translation="MILAIDAGNSRVKWATHDGGKFIRDGWVAYAEFDKLDGQWAGLP
                     PPSSIVIGNVAGDDISKKLSALCARWKVEPRWACSVRSQAGVFNRYEQPSQLGVDRWA
                     ALIGARGVCSGACVVANAGTAMTIDALGKQGEFIGGIVVPGFDLMHEALAANTARLSA
                     ERGTFFDFPRTTRDAITSGAIQALCGAVERMRNAMLLAGHEDPELIVSGGAGEIVARH
                     IGRPVRVIDKLVLEGLVRIAMEQP"
     gene            1014..1673
                     /locus_tag="VN496_12760"
     CDS             1014..1673
                     /locus_tag="VN496_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXF17871.1"
                     /translation="MKLIFFLLLAANLALAVVIFVREGLPNPDAQLVSQQFNADKIQI
                     LPPQLVSAPAAVAIAPARPAGVCLQWGNFGAAELPKAQAALDGLLFADRARKTDVPVL
                     TSYWVFIGPLRSRAELEKKTSELNKLGIIEFLPIVETGRWRFALSLGVFRTEEGAKNY
                     LEALRGKGVRSAQYGEREQRITQTAFLFRDLSETQKGQLTSLKASYPGSDVRSVDCPP
                     V"
     gene            complement(1680..3407)
                     /locus_tag="VN496_12765"
     CDS             complement(1680..3407)
                     /locus_tag="VN496_12765"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXF17872.1"
                     /translation="MFARFTFLRDTWRLIKPYWKSEERFIAWLLLAAVIILTLAMVYM
                     NVLFNDWYNSFYNALQEKDKTGFWKLMGRFCILATIYIVMAVYEFYLNQMLQIRWRRW
                     LTDNYLSEWLSDRAYYRLQLSGNPADNPDQRISEDMKFFVDQSLELSLGFLNAIVTLG
                     SFVGILWGLSGPLEISLNGNPVSVQGYMVWAALFYAIVGTWLTHQIGKKLIGLNFDQQ
                     RFEADFRFSLVRFRENAEGVALYKGEPDEFVGFRARFASVVDNWWRIMKRQKVLNFFT
                     IGYNQAAIIFPFLVAGNRYFAGTIQLGGLMQISNAFGHVQSSLSWFITAYPTFATWKA
                     TSDRLIGFHYAIERARAQARDESGAERIVGQQSDLVLENVSVALPDARPLVAASSLDV
                     KQGEHLLIRGPSGSGKSTLFRALAGIWPYSSGRIRLPKDGRILFLPQKPYLPIGTLKQ
                     VTVYPSVDITLPDEEIKNALSEVGLAQLRDRLDEQQNWSMQLSPGEQQRIAFARALLQ
                     QPDWLFLDEATSSLDEEAEQKLYRLLQEKLPRTTVISIGHRSTLQAFHKRSIEMKMDS
                     AGLRVLAPA"
     gene            complement(3430..3915)
                     /locus_tag="VN496_12770"
     CDS             complement(3430..3915)
                     /locus_tag="VN496_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXF17873.1"
                     /translation="MHAPPTFERKIVPPDELAARLRPIDRPLVFTNGVFDVLHRGHVT
                     YLAESRALGASLVLALNSDSSVKRLGKGPDRPVNTLADRLAVVAALESVSMVTWFDAD
                     TPLSLILNCRPDILVKGGDWPIEKIVGATEVQAWGGEVRSIAFQFPNSTTALLSRIRN
                     S"
     gene            complement(4172..4744)
                     /locus_tag="VN496_12775"
     CDS             complement(4172..4744)
                     /locus_tag="VN496_12775"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXF17874.1"
                     /translation="MRSTIHLVILASLIPLLQSCVPLVVGAGAAAGVMMAEDRRTNAT
                     MLEDQAIELKAGNRISEKYKDLGNISVTSFNRYVLLTGQVPTEEIKQDLSIVTLEVPS
                     VRNVQNEIVVSGVSSTTSRASDTLLTSEVKGRLAQNKDVGASHVKVVSENGTVFLMGL
                     VTRAEADAASQTAATTSGAQRVVKVFEYLD"
     gene            complement(4745..5335)
                     /locus_tag="VN496_12780"
     CDS             complement(4745..5335)
                     /locus_tag="VN496_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HXF17875.1"
                     /translation="MDLISRVSQHFTDSAQLKIKAKDVLAGPLVDAAERMVQCLMKEG
                     KILSCGNGGSAADAQHFAAELLNRFEKERSPLAAMALSTDTSTITSIANDYHYNEIFS
                     KQVRALGHPDDILLAISTSGNSPNVMEAIHAAHEREMDVVALTGRNGGKIATLLGPED
                     VHICVPAERTARIQEVHLLCLHCMCDAIDFLLLGAD"
     gene            complement(5402..5746)
                     /locus_tag="VN496_12785"
     CDS             complement(5402..5746)
                     /locus_tag="VN496_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXF17876.1"
                     /translation="MSERGAQAEEAAANFLTKRGLRLLERNYRCRFGEIDLVMSDGHT
                     TVFVEVRYRRNNTFGGSVESVTAAKRKKLLRAARHYMAAQKEFPDCRFDAVLLTGDTH
                     EIQWIENAFGED"
     gene            complement(5743..6840)
                     /locus_tag="VN496_12790"
     CDS             complement(5743..6840)
                     /locus_tag="VN496_12790"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HXF17877.1"
                     /translation="MQLRLAFRMWVRSSLYWWLIALATGFAGAQESTEVIVREPHIAV
                     ILPLQSNSYGKHAESVRLGILNAAANGPSGRLKVKIYATSEDPQQILTAYQRATSLGA
                     RAVIGPLTRNGVTSLAQSGIVSVPTLALNIPDADVSLPRDLYVYGLQIETEARQLAQF
                     ALRLGGTKAFIIVGDTTLAIRISQAFSEEWKKIGGAVTGQFGYTTDSAALTRLREQII
                     NANADIVFTSLDGARARFVRSYIGTAIPIYATSMVFTSNLDTLANFDLEGIRFLDMPW
                     LLQPDHPAVISYARPTAQRAGLDQERFYALGIDAYRLAQELLRPYESMEPLDGVTGTI
                     TLNNLHQFTRTLVPAQFSQGSAKMLEALPPR"
     gene            6856..7707
                     /gene="rsmI"
                     /locus_tag="VN496_12795"
     CDS             6856..7707
                     /gene="rsmI"
                     /locus_tag="VN496_12795"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXF17878.1"
                     /translation="MAEPTLYVVATPIGNLQDITLRALATLKTVDVIAAEDTRVSRKL
                     LSHFGIKTELISSHQHNEQRAARQLIEFLSKGKSVALISDAGTPAISDPGAVAVEQAR
                     AAGFRVVPIPGPSAITAAIAAGGLQWSSFCFHGFLPARKRERETALASLRLQRGLQVF
                     YEAPHRVVECVISMAAVLGADRRIGIARELTKLFEEIHVSRLDETVLWLQEDDNHRRG
                     EFVLMVEGYMAETTSENLSSNRALEILLRQLPLKQAVGLAAEITGVKKNALYQQALKL
                     KSQNDEQ"
     gene            7732..>8698
                     /gene="pyrC"
                     /locus_tag="VN496_12800"
     CDS             7732..>8698
                     /gene="pyrC"
                     /locus_tag="VN496_12800"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXF17879.1"
                     /translation="MNRITITKPDDWHLHLRDGDAMKSVVADSARRFGRAIVMPNLKP
                     PVTTVAEAREYRNRILSTLPPRSDFQPLMTLYLTDNTSAAEIHKAKASGIVSAVKYYP
                     AGATTNSDLGVTDLGRCVAAMKAMAEAGMPLLVHGEVVDPKVDVFDREKAFIEKTMMP
                     LIQQLPELRVVMEHITTGNAVKFILSAPDNVAATITAHHLLMSRNDMFKGGIHPHHYC
                     LPLLKREEHKMALVKAAISGNPKFFLGTDSAPHARHSKESACGCAGIYTAHAALELYA
                     EVFDAAGSLDKLEAFASFFGADFYGVERNRTTITLVRSAWTVPEEI"
BASE COUNT         1961 a         2329 c         2453 g         1955 t
ORIGIN      
        1 gccctgttcg ccgaaattct gctcgctctg gagaccgtat tgcgccggtt ctccacggaa
       61 ggatttgctt ctttcagcga ggagtggaac gcactgcatg cctatcaaga taaaatggta
      121 cgcatgagga tgccggacaa gtccgagata gaaggccggg tgcaaaatat cggcgacgac
      181 ggcgcactgt tgttgcatac gcgggccggg ctgcgcaaat tttacggtgg cgaaatgagt
      241 ctgcgcggca ttgcttgaga cggtcttcga taacacatca agccaaattt gatactagcg
      301 atcgatgccg gcaatagccg ggtgaagtgg gcgactcacg atggcggtaa gtttatccgc
      361 gacggctggg tggcgtacgc cgagttcgat aagcttgatg ggcagtgggc cgggctgccg
      421 ccgccgtcct ccatcgtgat cggcaatgtg gctggggatg acatcagcaa aaagctaagc
      481 gcgctgtgcg cgcgttggaa ggttgagcca cgttgggcat gcagcgtgcg ttcgcaggca
      541 ggcgtattta accgatacga acaaccatcg caacttggag tggaccgctg ggcggcattg
      601 ataggcgccc gtggggtttg ttccggtgct tgcgtcgttg ccaatgctgg caccgcgatg
      661 accatcgacg cgttgggaaa acagggcgag tttatcggtg gcattgtcgt tccaggtttt
      721 gacttgatgc acgaggcgct tgcagccaat actgcccggc tctcggcaga acgcggaacg
      781 ttcttcgatt tcccacgcac aaccagagac gcgattacca gcggagcgat tcaggcgcta
      841 tgcggtgcgg tcgaacgcat gcgtaacgcc atgctgttgg ccgggcacga ggatcctgag
      901 ttaatagtca gcggcggcgc cggcgaaatc gttgcacgcc atattgggcg accggtgcgc
      961 gtaatagaca agcttgtact ggagggactg gtgcgtattg cgatggagca gccgtgaagt
     1021 tgatcttttt cctgctgctt gctgcaaacc tggcgctcgc ggtggttatc ttcgtgcgcg
     1081 aagggctgcc gaatccggat gcgcaactcg taagccagca gtttaatgcc gacaaaatac
     1141 aaatcttgcc gccgcaattg gtatctgcgc cggccgccgt tgctattgca ccggcaagac
     1201 cagcgggcgt atgcctgcaa tggggtaatt ttggtgccgc tgaactgccc aaggcacagg
     1261 ccgcactcga tggattgttg ttcgcagatc gcgcacgcaa gaccgacgtg ccggtattga
     1321 caagttactg ggtgttcatc ggcccactcc gttccagggc ggaattggaa aagaagacaa
     1381 gcgaattaaa caaattggga atcattgaat ttttgccaat agtagaaacg ggccgatggc
     1441 gcttcgcact ttccctgggc gtttttcgga ccgaagaagg cgccaaaaac taccttgaag
     1501 ccctgcgagg caaaggcgtg cgatcggcac agtatggtga acgagaacaa agaatcacgc
     1561 agactgcctt tctgtttcgc gatctgagcg aaacgcaaaa gggtcaattg acgagcctga
     1621 aagcctcgta tcccggttct gatgtgcgca gcgtggattg tcctccggtc taagtcggtc
     1681 tacgcgggtg cgagcaccct taagcccgca ctatccatct tcatttcaat cgagcgctta
     1741 tggaaagcct gcagcgtcga tcgatggcca atgctaatca cggttgtacg cggcagcttt
     1801 tcctgaagta ggcggtagag tttttgctcc gcctcctcat ccagggagga tgtcgcttcg
     1861 tcgagaaaca gccagtccgg ttgttgcagc aaggcgcgcg cgaaggcgat gcgctgttgc
     1921 tctcccgggg aaagctgcat ggaccagttc tgctgttcgt ccaggcggtc acgcaactgc
     1981 gccagcccga cttcgctgag cgcgttctta atctcttcgt cggggagggt gatatcgacc
     2041 gagggataga cggttacttg cttcagggtg cctatgggca ggtagggttt ttgcggaagg
     2101 aaaaggatgc ggccgtcttt gggaaggcga atccgaccgc tgctgtatgg ccagattccg
     2161 gcaagtgcac ggaacagtgt gctcttgccc gatccggacg gcccgcgtat gagcaagtgt
     2221 tcgccctgtt tgacgtccag gctggatgcg gccacaagcg gccttgcatc cggcaaggcg
     2281 accgaaacat tttcaagcac cagatccgat tgctgtccca cgatgcgctc tgcgccagac
     2341 tcatcgcgcg cttgcgcccg tgccctttcg attgcgtagt gaaatccgat caaacgatcg
     2401 gaagtcgctt tccaggtcgc aaaggtcgga tatgcggtga tgaaccatga cagagaactt
     2461 tgcacatgac cgaacgcgtt ggatatctgc atcagcccgc caagctggat ggttccggcg
     2521 aaatagcggt tgccggcaac aaggaacgga aagatgattg ctgcctggtt atagcctatg
     2581 gtgaaaaagt tcagaacttt ctgccgcttc atgatgcgcc accagttgtc gacgacgctt
     2641 gcgaagcggg cgcggaaacc aacaaattcg tccggctctc ccttatacaa tgccacgcct
     2701 tccgcatttt cccggaaacg aaccagacta aaccggaagt cggcttcgaa gcgctgctgg
     2761 tcgaagttca ggccgatgag tttttttccg atttggtggg tcagccacgt gccgacgatg
     2821 gcataaaaca gcgcggccca aaccatgtag ccctgaacgg acacaggatt gccgttgagc
     2881 gaaatttcca gcggtcctga caatccccaa agaataccca cgaaggagcc gagcgtgact
     2941 atcgcattca agaatccaag cgacaattcc agggactgat cgacaaaaaa tttcatgtcc
     3001 tccgaaatcc gctgatccgg gttgtcggcc ggattgccgc tcagttgcaa ccggtagtac
     3061 gctcgatcgc tcagccattc cgaaagatag ttgtcagtaa gccaacgccg ccagcgaatc
     3121 tgcagcatct ggttcagata gaactcgtaa acggccatga caatgtagat ggtcgccaaa
     3181 atgcagaatc ggcccatcag tttccagaac ccggtcttgt ctttctcctg aagcgcgtta
     3241 tagaaggagt tgtaccaatc gttgaacagc acattcatat aaaccatcgc cagggtcaat
     3301 atgatgacag cggcaagcag cagccaggcg atgaagcgtt cctcagattt ccagtacggt
     3361 ttgatcaggc gccatgtatc gcgtagaaaa gtaaagcggg caaacatgaa tagttcctcg
     3421 tggaagcaat caggagttac gaatgcggga aaggagggcg gtagttgaat ttgggaactg
     3481 gaaggcaatg gatcgcactt ctccacccca ggcttggact tctgttgctc ccacaatctt
     3541 ttcaataggc caatctccgc ccttcaccag aatatccggg cggcagttca gtatcagcga
     3601 taacggcgtg tctgcatcaa accaggtcac catgctcacg ctttccagcg ctgcgacgac
     3661 ggctagtctg tctgccaaag tatttaccgg gcggtccggc ccctttccca gccgcttgac
     3721 cgaggaatcc gagttcagtg ccagaaccag acttgcgccc aatgcgcggg attcggcgag
     3781 gtaagtgaca tgcccacgat gcagcacatc aaagaccccg ttggtgaaca ccagcggccg
     3841 gtcgattgga cgcagccgag ctgccaactc atccggcgga acgatctttc tttcgaaggt
     3901 tggtggggca tgcattggag tgcccgcaat catacagggc tggacggagg gtgccagtgc
     3961 cagcccgctg ggaaaagttc ttacgtgatt gcgccgaatc cgaaaccgcg ctgaagtctt
     4021 ggatgatcag cgcggtggaa tcagactcat aagactgctc ggtttcgaca gtgaatcacg
     4081 aaacagctcg caaactaatg ttggagactg tacgccgcct gattacccca gaatgtaagc
     4141 gttcaataga ggctgttggg cgaccgaatc atcagtcaag gtactcgaat accttgacga
     4201 ctcgttgtgc tccactcgtg gtagcggcag tctgcgatgc ggcatcggcc tccgctcgcg
     4261 tcaccaggcc catcaggaat acggtgccgt tttcagagac caccttgacg tggctagcgc
     4321 ccacgtcctt gttttgcgca agacgcccct tcacttcgct cgtcagcagc gtatcgctcg
     4381 ctctcgatgt tgtggagctc acaccgctga caacaatttc gttttggaca ttgcgcacgc
     4441 tcggcacttc aagtgtcacg atcgatagat cttgcttgat ctcttccgtc gggacttgtc
     4501 ccgtcaacag aacgtagcga ttgaaactgg taacgctgat attgcctaga tccttgtatt
     4561 tttcgcttat acgattcccg gcctttagtt caatggcctg atcttccagc atcgttgcgt
     4621 ttgtgcgccg gtcttctgcc atcatgactc cggcagctgc gccggcgccc accaccaatg
     4681 gcacgcagga ttgaaggagg gggataagcg aagcaagtat taccagatgt atcgttgatc
     4741 tcattcagtc tgctcctagc aacagaaaat cgatcgcatc gcacatgcaa tgcaggcaca
     4801 gcagatggac ttcctggatg cgcgcagtgc gctctgcagg tacgcaaatg tgtacatcct
     4861 caggtccaag caaagtggca atcttgcctc cgttccgtcc cgttagcgcg accacgtcca
     4921 tttcgcgttc gtgcgctgcg tgaattgctt ccatcacatt gggtgaatta ccgctggtcg
     4981 agattgcgag caggatgtca tccggatgac ctagagcacg aacctgcttg gaaaatatct
     5041 cgttgtagtg gtaatcgttc gcaatcgaag taattgtgga ggtatccgtg ctcagggcca
     5101 tagccgcaag cggcgaacgt tccttttcga accggttcag caattctgcg gcgaaatgct
     5161 gtgcatctgc ggccgatccg ccgttaccac aagagagaat cttgccttct ttcatcaggc
     5221 actggaccat gcgctccgcg gcgtcgacaa gtggcccggc gagaacatcc tttgccttga
     5281 tcttgagttg ggcgctgtcg gtaaaatgtt gactgacgcg gctgatcaaa tccattggag
     5341 gaattctata aattgaatgg ggcgaattat tgcacaagct ttgtcgacac aaagctgcaa
     5401 attaatcttc accgaatgcg ttttcgatcc attgaatctc atgagtgtcg ccagtgagca
     5461 acacggcatc gaagcggcag tcgggaaatt ccttttgggc cgccatatag tgccgcgccg
     5521 cgcgcagcaa tttttttcgt ttggccgccg ttacactttc aaccgagcca ccgaacgtat
     5581 tgttgcgccg ataacggact tcaacaaaca cggttgtgtg cccgtcgctc atgaccagat
     5641 cgatttcgcc gaagcggcag cgatagtttc gctccagcaa tcttagcccg cgcttggtta
     5701 aaaaatttgc ggcggcttcc tcggcctgcg cgccgcgctc gctcatcgag gtggaagtgc
     5761 ttccagcatc tttgcgctgc cctgggagaa ctgtgccggt accaacgtgc gagtaaattg
     5821 atgcaaatta ttcagagtga tcgttccggt caccccatcc aacggttcca tcgattcata
     5881 cgggcgcaac agttcctggg cgagccggta ggcatcgatt ccgagcgcgt agaatcgttc
     5941 ctgatccagc cccgcacgct gtgcagtagg gcgcgcgtaa ctgattactg cgggatgatc
     6001 aggctgcagc agccatggca tgtccaggaa acgtatgcct tccaggtcga agtttgcaag
     6061 cgtgtccaag ttggacgtga ataccattga cgtggcgtaa atggggatgg ctgtaccaat
     6121 ataggagcgt acaaatcgag cgcgggcgcc atcaagtgat gtaaaaacga tgtccgcatt
     6181 ggcgttgatg atttgttcgc gcaagcgcgt aagtgcggct gaatccgtcg tatagccgaa
     6241 ctggccagta actgccccgc ctattttctt ccattcttct gaaaacgcct gtgatatgcg
     6301 gatcgctaat gtcgtatcgc ccacgataat gaaagcttta gtgccgccaa ggcgcaacgc
     6361 gaactgcgct aactgtcgcg cttccgtttc gatttgcagg ccataaacat ataggtccct
     6421 tggcagcgat acgtctgcat caggaatgtt cagtgccagc gttggaacgc tcacaatgcc
     6481 gctttgcgcc aatgacgtca cgccgtttcg cgtcaacgga ccaatgaccg cgcgtgctcc
     6541 aagcgatgtg gctcgctggt aagccgtcag aatttgttgc gggtcctcgc tggtagcata
     6601 gattttgacc tttaacctgc ctgacgggcc gttggcggcg gcattcagga tcccaagtcg
     6661 aactgattcg gcgtgcttcc cgtacgaatt ggactgcagc ggtagtatta ctgcgatgtg
     6721 aggctcgcgt acgatgactt cggtactttc ttgcgcgccc gcgaaccctg tggctaaggc
     6781 gattagccac cagtatagtg atgagcgaac ccacatacga aaggccaggc gaagttgcat
     6841 agcgaaaaga cggaagtggc cgagccgaca ttatatgtgg tggctacccc gatcggaaat
     6901 cttcaggaca ttaccctgcg cgctttggca acgctcaaga cggtcgatgt tattgcagcg
     6961 gaggatacgc gcgtttctcg caagctcttg agccattttg ggataaaaac ggagttgatt
     7021 tcgtcccatc aacataacga gcagcgagcg gcacgtcagt tgatcgaatt cctgtcgaag
     7081 ggaaaatccg ttgcccttat ttccgatgca ggcacgcctg ccataagcga tccgggagct
     7141 gttgccgtgg agcaggcgcg agctgccggt tttcgagtag tccccattcc ggggcccagc
     7201 gcaataacgg ccgctattgc ggcaggcgga ctgcaatggt cgtcattctg tttccacggc
     7261 tttctgcctg cgcgtaagcg tgagcgcgag acagcgcttg cctcacttcg gttacagcgc
     7321 gggctccagg tgttttatga ggctccccat cgcgtggtcg agtgtgtaat cagcatggct
     7381 gccgtcttgg gcgccgatcg ccgtattggt attgcgcgcg agctcacgaa actatttgaa
     7441 gagatccacg tgtcccgcct ggacgagacg gtgctctggt tgcaggaaga cgataaccat
     7501 cgtcgcggcg agttcgtcct catggtggaa ggttatatgg cggaaacaac atcagagaac
     7561 ctgagctcga atcgtgcgct ggaaattttg ctccgccaat tgccgctgaa gcaggcagtc
     7621 gggttggctg cagaaatcac aggcgtgaag aaaaatgcgc tgtaccagca agcgctgaaa
     7681 ctcaagagcc aaaatgatga acaatagagc ggccaatttg caagtgccta tatgaaccgc
     7741 ataaccatta ccaagccgga cgattggcat ctgcacctcc gtgacggaga tgcgatgaaa
     7801 tcggtcgttg ccgattcagc aagacgcttt ggtcgtgcca tcgtaatgcc aaacctcaaa
     7861 cccccggtga ctacggtcgc cgaagccagg gaatatcgca atcggatctt gtcgactttg
     7921 ccaccgcgga gtgattttca accattgatg acgctgtacc ttactgacaa caccagcgcg
     7981 gccgaaattc acaaggcaaa agccagcggt atcgtcagcg cggtcaagta ctatcccgcc
     8041 ggggcaacga cgaattccga tctgggcgtg actgatctcg gtagatgcgt cgcggcaatg
     8101 aaggccatgg ctgaggccgg aatgcctctt cttgtgcacg gcgaagttgt tgacccgaag
     8161 gtcgacgtat tcgatcgtga aaaagccttc atcgaaaaaa ccatgatgcc tcttattcaa
     8221 caattgccgg agcttcgagt tgtgatggaa cacatcacga ctggcaacgc ggtcaagttt
     8281 attctgagcg cgccggacaa tgtggctgcc accattacag cgcatcatct tctgatgagc
     8341 cgtaacgata tgttcaaagg aggcattcat cctcaccact actgcctgcc actcttgaag
     8401 cgcgaagagc acaagatggc gctggtcaag gctgccatct ccggcaatcc aaaatttttt
     8461 cttgggaccg acagcgcccc tcatgcacga catagcaaag aatccgcttg cggatgcgct
     8521 ggcatatata ccgcacatgc cgcccttgag ttgtacgccg aagtgtttga tgctgccggc
     8581 agcctggata aactcgaggc gtttgccagt ttttttggtg cggatttcta cggtgtggaa
     8641 agaaatcgga caacgatcac gcttgtccgc agcgcctgga ccgtgcctga ggaaattc
//
LOCUS       DATMZQ010000400        13614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1353648, whole genome shotgun sequence.
ACCESSION   DATMZQ010000400 DATMZQ010000000
VERSION     DATMZQ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353648"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="VN496_12805"
     CDS             complement(<1..1748)
                     /locus_tag="VN496_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF17880.1"
                     /translation="MKPTDINVPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYGDAY
                     MVNWNSNVFPGILGRTCDRPCEPACRRGRVEEQNQPKPEPVAICRLKRVAADNKGDVR
                     SQLPKAPAQKNGKRVACIGAGPASLAVARDLAPLGYEVVVFDQDPKAGGMIRTQIPRF
                     RLPEEVIDEEVGYVLGTGVIFRGGVRIDSLKRILSEGFDAIFVGSGAPRGRDLELPGR
                     MEAAKNIHIGIDWLSSVSFGHVDAIGRRVIVLGGGNTAMDCCRTSRRLGGNDVKVIVR
                     SGFEEMKASPWEKEDAMHEGIPILNYLVPKSFQHRNGRLTGMVFEKVRAMFDEQGRRK
                     LIPTGEADQLFECDDVLIAVGQENSFPWIERDAGIAFDKSGMPVVDKTTFQSSHPRVF
                     FGGDAAFGPKNIIWAVAHGRDAAISIDQLCNGADVHMRPAPRVNVTSQKMGIHEWSYD
                     NDISSELRHKVPLKDQAIALKDIKVEVELGFDVKLAFAEAQRCLNCDVQTVFSPPLCI
                     ECDACVDICPMDCITFTANGEEQELRGRLNAPAETLSQSLYVSDVLKTGRIMAKDEDV
                     CLHCGLCAERCPTGAW"
     gene            complement(1903..3144)
                     /locus_tag="VN496_12810"
     CDS             complement(1903..3144)
                     /locus_tag="VN496_12810"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HXF17881.1"
                     /translation="MHLIQPFAGLRPTDGHAADVAAPPYDVVSTEEARARANGKPWSF
                     LHISKPEIDLPPGTDPYAPEVYAKAADTLQRMLLHKILERDTQPCYYVYRLLMDGHAQ
                     TGVVGAASIAHYDSNRIRKHEFTRPDKEDDRVRQIEALNAQTGPVLLAYPESPEVDAL
                     LVRAAASEPVADVVAETGVRHTVWRIAQSSIINRLTMAFDAMPAIYIADGHHRSAAAS
                     RVAASRRAVNPRHDGTESYNFFLTVAFPHHHMRILPYNRVVTDLNRLSRQEFLSKLGQ
                     SFSLHAAPKAVVPAKPGEFGLYLPAQWYRLNVHPAVVPARDPVERLDVSLLANLVMAP
                     VLAITDPRRDKRIDFIGGIRGLSELEQRVDSGSMAAAFSMYPTRMEDLMSVADAGEIM
                     PPKSTWFEPKLADGLVSHVLD"
     gene            complement(3225..4703)
                     /locus_tag="VN496_12815"
     CDS             complement(3225..4703)
                     /locus_tag="VN496_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXF17882.1"
                     /translation="MNANVARLAVDDSATFQNLIGGKWLDASDGHMLDMVSPSDGRAF
                     AKIARGTEADIHAAVQAARQAFEEGAWGKMTAVERGRLMTKLGEAILAHHEELSQLEG
                     RDCGKPMKQARADITAAARYFEFYGGAADKFHGDTLPFLDGYTVAILREPKGVTGHII
                     PWNYPAQMFGRSLAPALAMGNAVVMKPAEDACLTPVRIGQLAQAVGFPPGALNIVTGI
                     GEEAGAALASHPGIDFLSFTGSPEVGTLVQKAAAANHIGCTLELGGKSPQIVFEDADF
                     DATVPVVVNAIVQNAGQTCSAGSRVLVQRSIYDQFVAQVVARFKLLRVGSHSMDLDCG
                     PLISSNQRERVHGFIDRAKKAGIPVLAQAELAEGLPAGGFYVAPVLFGPVPRDNALAC
                     DEVFGPVLSIIPFDDEVDAVKLANSTSFGLVAGVWTRDGARQMRVARKMRCGQVFVNG
                     YGAGGGIELPFGGVKKSGHGREKGMEALYEFSQTKTIVFKHG"
     gene            complement(4715..5392)
                     /locus_tag="VN496_12820"
     CDS             complement(4715..5392)
                     /locus_tag="VN496_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXF17883.1"
                     /translation="MQLRNMCRLIVLILAASLGLSACQTTDPYTGEQKINSASKGAAI
                     GVAAGAVVGAISGSDAQERRKRAMIGAGIGGLAGAGVGAYMDKQEDDLRKQMEGTGVS
                     VTRQGDNITLNMPGNITFKTGSAELSPSFPKVLDGVVMVLKKYEKTIIEVAGHTDDVG
                     KAEYNQALSERRAHTVTNYLVAKGVKTERTISIGAGENHPVASNANETGRAANRRVEL
                     SLLPLKQ"
     gene            complement(5578..6015)
                     /locus_tag="VN496_12825"
     CDS             complement(5578..6015)
                     /locus_tag="VN496_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HXF17884.1"
                     /translation="MKNNMLAVLRAILVIAAVVMAQTNARADNTIKTVYHLQDDRVAT
                     LAMNNIANHVSADPGVKIVVVALSTGVRAFTFGAQDAGGRPYAEWVEQLKANGVEFRI
                     CQNSMKALKLTQKDLIENVSTVPSGVAEIARLQAKEGYVYIRP"
     gene            complement(6044..6646)
                     /locus_tag="VN496_12830"
     CDS             complement(6044..6646)
                     /locus_tag="VN496_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXF17885.1"
                     /translation="MDTVLFDLGAVLIDWNPRYLYRPLFKGDEQAMEHFLAEIAPPWW
                     NLEIDAGKTFDQAVAERAALHPAHSELIALWRDGWETMLRDEIPGSVAILRDLRSKGH
                     RLHALTNWSAETFPIARRRFEFLQWFEHIVVSGEVGLAKPDPRIFGLAIERCALDPGR
                     TVFIDDSPRNVEAGRNAGLHALHFTDPDRLRVDLMQLGML"
     gene            complement(6647..7372)
                     /locus_tag="VN496_12835"
     CDS             complement(6647..7372)
                     /locus_tag="VN496_12835"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HXF17886.1"
                     /translation="MKPAPIAPQILDQLRRISTATLTSQLFKLGFRNTYLNGVKPLNP
                     TLRMAGEAVTVRFVPAREDLATFEAIGKPGYPQRKAIDEILPGQVLVLDCRNIDSAAA
                     GGEILMSRLQARGASGAVTDGAMRDYVSIQKLGFAVFAKNMAATAHIHKHVAVDINVP
                     IGCAEVLVMPGDIMVGDDEGVVCIPRHMAEQVAKSGLEMEELETFVLEKVQAGAPISG
                     TYPPSGPVQAEYEAWKKKRASGR"
     gene            complement(7471..8361)
                     /locus_tag="VN496_12840"
     CDS             complement(7471..8361)
                     /locus_tag="VN496_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXF17887.1"
                     /translation="MPVIMRLPFLLMGLAICVSAAADDLVGKMGDVELRSSELMLILD
                     AQAPDARRQLATDPAALERLVRSELMRKAVLHEAKQKGWDKRKELEPMIERARDQVIV
                     SSFVANVAKPGDGYPSEEEIKQFYESNKAQLVAPAQFQLAQIFLPAPEGTDKAKADEA
                     KKKIGELSAKLGKSGTDFAKLAKENSAHKESAEKGGELGWVSEEQMVPEIRRAVSGMA
                     KGEVSPPIKSAAGWHLIKLLDKKAAGTRALSDIRPNLVIAMRNRKAQDMERTYLEALS
                     IKLPPTINQIELGKLQAGLR"
     gene            complement(8529..8933)
                     /locus_tag="VN496_12845"
     CDS             complement(8529..8933)
                     /locus_tag="VN496_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HXF17888.1"
                     /translation="MAAKKKTAKKSAKKSVKKAAPKKAAAKKTAKKAAKKPAKKAAPK
                     KKASGVKRKPNAAFMKAMTPSASLAAVIGSAPLPRTEVTKKLWAYIKKNDLQDSKNRR
                     NINADDKLKDVFGGKKTVSMFEMTKLVAGHLK"
     gene            complement(9291..10154)
                     /locus_tag="VN496_12850"
     CDS             complement(9291..10154)
                     /locus_tag="VN496_12850"
                     /inference="COORDINATES: protein motif:HMM:NF027655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HXF17889.1"
                     /translation="MKQLIAYVYVVLALAGCATPDQLRQTEAQTAEQGYAVKSLRAGQ
                     DSNASLIKEVRAELSQSQQSMHALEASVVETQARADTAQATSKELLASVVAAREEQRR
                     QLDQNGAAFAELRRRNAEMDAKLQVQQRALEQSATASVDATRRLNEIDTKLQGVTRRA
                     AALETKSGNAQVNDVILTQQITALSKEVAETRALINSEGLLQLMREVEDLRRNSASLR
                     GSIEELQKSQADAAVQIRNFYVDLDTRIRLLKQSPGQQAVKQNSPAAQSVPAGPTQDA
                     GAAPLVQPYRQ"
     gene            complement(10168..11097)
                     /gene="pal"
                     /locus_tag="VN496_12855"
     CDS             complement(10168..11097)
                     /gene="pal"
                     /locus_tag="VN496_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXF17890.1"
                     /translation="MVDASLFSITRNIFMILVCTALASCATTQRSSSGAGGQTAAPIS
                     EAGVGTQEGATADAATKSLQPSADNPGSALPPAGKPATSSTESSAAGGTGQPKTASAN
                     AGAEEAQLKRQLAEQEAEISKLRTTQETEAAQREQQQAQGGDPNGSSPGGKVAAPAAV
                     TPEQVGKPETAAAHRDDDIAIFPAGEKSGQAAVESLAPAPAADARSIFFEYDQSTIPK
                     HYDTMLLQNAAYLKASPRVSFEVQGNCDERGSREYNLALGARRAQAVKRALELAGADG
                     GRIQVVSFGAEKPIANGKDEESYSKNRRVDIVY"
     gene            complement(11312..13417)
                     /locus_tag="VN496_12860"
     CDS             complement(11312..13417)
                     /locus_tag="VN496_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXF17891.1"
                     /translation="MNDVLRDALRTVLNPKSVAVIGASENENKIGGRPLLYLARHGFK
                     GRVYPINPKRAEIQGFKAYPSLADLPDVPEAAIVALAGDAAIAAVEQCAQLGVKTAVV
                     MSSGFGETDAVAGKAKEARMRDAARAAGMRVVGPNSQGLANFGTGAILSFSTMYIEAP
                     PLDGPIAVVSQSGAMSVVPYGLLRAQGLGVRHSHATGNDADVTVSELASVVAEDPDLK
                     LLLLYLESIDDSFFLAETARIAHARNLPIIALKSGRTLAGQEAAKSHTGALANEDRTV
                     DAFLAKQGIWRARDTAELVASAQMYLKGWKPKGRRLVAISNSGAVCVMAADAASTLGM
                     PLAKLADETRAGLGKILPGFATTTNPIDITAALLSNSGLFSQILPVLARDSAADAFLI
                     GIPVAGQGYDVDAFARDSANFAKATGKPLVAAIPQSNIAAHFKAYGLPVFPTEYEAVA
                     ALNQFLSHGELMQAWKPAASPQRYAPPTGPATMLNEAESLTLLATAGVPVVKFRLCHN
                     AEEAVQALAQLGGRVALKGCSADVAHKSELGLVHLGADQAGQVRAAFTEIKKALLKHG
                     ARFDGVIVAQMAQGRRELMIGARVDPVFGPVVLVGDGGKYVEAMPDIQLLIAPFCAAE
                     VKQAFARLRIAPLLAGVRGEAALDVDAFCSAVLSVSRLMTDRQAGIANLDLNPILIGA
                     EGEGCIALDAVVYVQRQNH"
BASE COUNT         2616 a         4152 c         3772 g         3074 t
ORIGIN      
        1 tcccaagcgc cggtcggaca gcgctcggca cacaacccgc aatgcagaca gacatcttcg
       61 tccttggcca tgatacggcc ggttttcagc acgtcagata catacaaact ctgcgacagc
      121 gtttcggccg gtgcgttcag ccggccgcgt agttcttgct cttcaccgtt ggcagtaaac
      181 gtgatgcaat ccatcggaca gatatccaca caggcatcgc attctatgca aagcggcggc
      241 gaaaacaccg tctgcacatc gcagttgaga cagcgttgag cttcagcgaa cgccagtttc
      301 acatcgaaac ccagttcaac ttccaccttg atatctttga gcgcgatggc ctggtccttc
      361 agcggcacct tgtggcgcag ctcactggaa atatcgttgt catagctcca ctcgtgaatg
      421 cccatttttt ggctggtcac attcacccgc ggagcaggcc gcatgtgcac atctgcacca
      481 ttgcatagct ggtcgatcga aatggctgca tcgcgcccat gcgcgaccgc ccagatgatg
      541 ttttttggac cgaatgccgc gtcgccaccg aaaaagaccc ttgggtgtga cgactggaaa
      601 gtggtcttgt cgacgaccgg catgccggat ttatcgaatg cgatgcctgc atcgcgctca
      661 atccacggga atgagttttc ctgcccgacc gctatgagca catcatcgca ttcgaacagt
      721 tggtcggctt cgccggtcgg tatgagcttg cggcggccct gttcatcaaa catcgctctg
      781 actttttcaa acaccatccc ggtcagccgg ccattgcggt gctggaacga ttttggcacc
      841 aggtagttca ggatcgggat gccttcatgc atcgcatctt ccttctccca ggggctggct
      901 ttcatttctt cgaacccgct gcgcacgatg accttgacgt cgttgccgcc caggcgccgt
      961 gaagtccggc agcaatccat tgccgtattc ccaccgccca gcacgatcac gcgcctgcca
     1021 atggcgtcga catgaccgaa tgagacagaa gatagccaat cgatgccgat gtggatgttc
     1081 tttgcagctt ccattcgccc tggcaactcc aggtcccggc cgcgtggcgc tcccgaacca
     1141 acgaatatgg cgtcgaagcc ctcggacaga atccttttca ggctgtcgat gcgtacgccg
     1201 ccgcggaata tgacgccggt accgagcaca taaccgactt cctcgtcgat cacctcctcg
     1261 ggcaagcgga agcgagggat ctgagtgcgg atcatgccgc cggctttcgg gtcctggtcg
     1321 aataccacaa cctcgtatcc gagcggcgcg agatccctcg ccacagcgag agatgccggc
     1381 ccggcaccga tgcaggccac gcgcttaccg ttcttctgcg ctggggcctt tggcaattga
     1441 ctgcggacat ctcccttgtt gtcggccgcc acgcgcttca aacggcaaat agcaaccggc
     1501 tccggcttgg gctgattctg ctcttcgact cttccgcgcc gacatgccgg ctcacaaggc
     1561 ctatcgcagg tacgtccgag aatgccgggg aacacattgg aattccagtt caccatgtag
     1621 gcatcgccat agcggccggc agcgatcagc ctgatgtatt ctggaacggg ggtgtgggcc
     1681 ggacatgccc actgacaatc gacgacctta tgaaaatagt cgggtacgtt gatatcggtg
     1741 ggcttcaaaa aaaatttcct ccgcctgggc gccttactgc gaattgttat agcccgcgga
     1801 tcccaagcat ttccgacgcg gaacacctgc aagaagcggg tcttgactgc ccaattggtg
     1861 cgtagtgtaa cgctttgtta cccctcgcgt tgacaggccg tactaatcca gcacatgcga
     1921 caccaaccca tcggccaact tcggctcgaa ccaagtcgat tttggcggca tgatttcccc
     1981 tgcatccgcg accgacatga gatcttccat gcgcgtcggg tacattgaaa aagccgctgc
     2041 catgctaccg ctatctaccc gctgctccaa ttccgacaaa ccccggatgc cgccgataaa
     2101 atcaatgcgc ttgtcgcggc gcggatcggt tatggccaac acgggcgcca tgaccaaatt
     2161 cgccagcagg ctgacatcca gccgttcgac cggatctctg gccggcacga cagccgggtg
     2221 gacattcaag cgataccact gcgccggtag gtaaagtccg aattcacccg gctttgccgg
     2281 tacaactgcc ttgggggccg cgtgcagcga aaaactttgg ccaagtttcg acaagaactc
     2341 ctggcggcta agcctgttca ggtcggtcac cacgcggttg tatggcagga tgcgcatatg
     2401 atggtgcgga aacgctaccg tcaggaaaaa attatagctt tcggttccat catgtcgcgg
     2461 atttaccgca cggcgcgatg cggccacgcg tgacgcggct gcggagcgat ggtggccgtc
     2521 cgcgatatag atcgcgggca tggcgtcgaa tgccatagtc aaacgattaa ttatcgacga
     2581 ctgcgcgata cgccaaaccg tatggcgcac tccggtctcc gcaacgacat ccgcgactgg
     2641 ctcactggcc gccgcgcgaa ccagcaacgc atctacctcg ggtgattcgg gataggcaag
     2701 caaaaccggg cccgtttgcg cgttaagcgc ttcgatctgc cgaacccggt cgtcctcttt
     2761 atcgggtctt gtgaactcgt gtttgcggat gcgattgctg tcgtaatggg ctatggaagc
     2821 tgccccaacc acaccggttt gagcatgtcc atccatcagc aagcggtaaa cgtagtagca
     2881 cggttgcgta tcacgctcga gtatcttgtg cagcaacatg cgctgcaggg tatccgcggc
     2941 cttggcatag acttccggcg cataaggatc ggtgccaggc ggcagatcga tttcaggttt
     3001 ggaaatgtgc agaaaactcc acggcttccc attggcgcgt gcgcgagctt cctcggtgga
     3061 aaccacgtca tacggtggcg ccgcgacatc ggccgcgtgt ccgtctgtgg gtcgcaatcc
     3121 ggcgaaaggc tgaataagat gcaaaccgct cctccttcga aaaaacggcc tgagattatc
     3181 aggccgtttt gagacgatca ggcacgatgg gccgacaggt acaatcaccc gtgcttgaat
     3241 acgatcgtct tggtctggga aaactcatac aacgcttcca tgcctttttc cctgccgtga
     3301 ccacttttct tgacacctcc gaacggcagt tcaatacctc cgcctgcgcc atagccgttc
     3361 acgaagactt ggccacaacg catttttcgc gctacccgca tttgtctggc cccgtcgcgt
     3421 gtccacactc cagccaccaa accgaatgag gtcgagttag cgagtttcac cgcgtccact
     3481 tcatcgtcga acgggatgat ggagagcaca ggcccgaaca cttcatcgca agcgagcgca
     3541 ttatcgcgcg gcactggccc gaacaatacc ggcgcaacgt agaagccgcc ggcgggcaag
     3601 ccttcagcaa gttctgcttg tgcaagcact ggaatgccag cctttttggc acgatcgatg
     3661 aagccgtgca cgcgttcgcg ttggttactg gagatcaacg gcccgcaatc caggtccatc
     3721 gagtgactgc cgactcgcaa cagtttgaag cgggcgacga cctgcgccac gaactgatcg
     3781 taaatcgatc tctgcacgag cacgcgactt cccgccgaac aggtctgccc ggcgttctgc
     3841 acgatcgcgt tcacaaccac cggcacggtg gcgtcgaaat cggcatcctc gaacacgatc
     3901 tgcggcgatt tgccgcccag ttcaagcgtg cagccgatat gatttgcggc ggcggccttc
     3961 tgcaccagcg tgcccacttc aggcgagccg gtaaacgaca ggaaatcgat tccgggatgg
     4021 cttgccagcg ccgcacccgc ttcttctccg ataccggtaa cgatattcag cgcaccgggt
     4081 ggaaagccga ccgcctgcgc gagttgtccg attcgtacgg gtgtgaggca cgcgtcttct
     4141 gcaggtttca tgactactgc attgcccatg gcaagcgcag gtgcaaggct gcgcccaaac
     4201 atctgcgcag gataattcca gggaataata tgtccggtga cgccctttgg ttcgcgcagg
     4261 attgcgaccg tgtagccatc gaggaacggg agagtgtcgc cgtggaattt atcagccgct
     4321 ccgccgtaga actcgaaata gcgggcagcc gcggtaatgt cggcgcgcgc ctgcttcatt
     4381 ggcttgccgc aatcgcgccc ttcgagctgg gagagttcct catggtgtgc gaggattgct
     4441 tctccaagtt tcgtcatcag gcggccacgt tcgactgctg tcatcttgcc ccaggcacct
     4501 tcctcgaatg cctgccgtgc ggcttgcacc gctgcatgga tatcggcttc cgtgccgcgt
     4561 gctatttttg cgaatgcgcg cccatcgctg ggactcacca tgtccagcat gtggccgtcg
     4621 gacgcgtcca gccatttgcc accgatcagg ttctggaaag tcgcactatc atccacggcc
     4681 agccgcgcaa cattggcatt cattgcagtt tcctttactg tttaagtggc aagagcgaga
     4741 gttcgactct ccggttcgcc gcgcgccccg tctcgtttgc gttgctcgct accggatggt
     4801 tttcgcccgc acctatgctg atggtgcgtt ctgtcttgac tccttttgcg accaggtagt
     4861 tcgttaccgt gtgagcacgc cgctcggaga gtgcctggtt atattccgcc ttgccgacat
     4921 cgtccgtatg gccagcaact tcaatgatag tcttttcata tttcttgaga accatgacga
     4981 cgccgtccag cacttttgga aacgacgggc tcaattcggc gctaccggtc ttgaacgtaa
     5041 tgttgcccgg catattcagc gtgatgttat cgccttggcg tgtcaccgaa acgccggtac
     5101 cttccatttg cttgcgtaaa tcgtcttcct gcttatccat gtaggcaccg acgcccgcgc
     5161 ctgccagccc cccgatgccc gcgccgatca ttgcgcgctt cctgcgctcc tgtgcgtcgc
     5221 tgccgctgat cgcgccgacc actgcgccgg cggcaacgcc aatggctgcc cctttgcttg
     5281 ccgaatttat tttttgctct cccgtgtacg ggtcggtggt ttgacaggcg ctcaatccaa
     5341 gacttgcggc gagaatcagg acgatcaatc ggcacatatt acgaagctgc atcagtcgaa
     5401 ttcctttcat gaagcgttag tggattttct gggaagctcg gagcgaatgg cgtctcgagc
     5461 ggggcgcaat tctaccaaca caggaggtgc tgtttggtgt cgcctgcgtc gggaaccagc
     5521 gacactatcc gagcgcagcc gattgccagc gagaaccggc tctttgcccg cgatagacta
     5581 cggccggatg tagacgtatc cctctttggc ctgcaggcgc gcgatctcgg ccacgccgga
     5641 agggacggta ctgacgtttt cgatgaggtc tttttgcgtc aacttcaggg ctttcatgct
     5701 gttctggcaa atgcgaaact caacgccgtt ggcttttagc tgctctaccc actctgcgta
     5761 aggccgccca cccgcgtcct gcgcaccaaa tgtgaaggcg cgcactcccg tggacaaagc
     5821 caccacaaca atcttgaccc cgggatctgc gctgacatga ttggcaatgt tgttcatcgc
     5881 cagcgtagcg acccggtcgt cttgaaggtg atacaccgtt ttgatggtat tgtccgcgcg
     5941 cgcattggtc tgggccatca cgacggctgc gatcaccaaa atcgcgcgca agacagccag
     6001 catattgttt ttcattgagt gcatctcctt ggatggggtc cagctatagc ataccgagct
     6061 gcataaggtc cactcgcaac cggtcgggat cggtgaaatg caacgcatgc aagccggcat
     6121 tccggccggc ctccacatta cgcgggctgt catcgatgaa taccgtgcgt cccgggtcaa
     6181 gtgcgcagcg ttcaatggcc aagccaaaaa tgcgcggatc gggttttgcc agtcccacct
     6241 cgccggacac tacgatatgc tcgaaccatt gcaaaaattc gaatcgtcgg cgtgcgatcg
     6301 ggaatgtttc ggccgaccag ttcgtcagag catgcagccg atggcccttg ctgcgcagat
     6361 cgcgcagaat ggccacgctt cccgggattt catcgcgcag catggtttcc cagccgtcgc
     6421 gccacagggc aattaattcc gaatgtgccg ggtgcagggc ggcccgctcg gcaaccgcct
     6481 gatcaaaagt cttcccggcg tcaatctcga ggttccacca gggaggcgct atctctgcca
     6541 gaaagtgttc catcgcctgc tcgtcgccct tgaacagcgg ccggtacaga tagcgcggat
     6601 tccagtcgat cagcactgcg cccaaatcga acagtacggt atccattcaa cgccccgatg
     6661 cgcgcttttt cttccatgcc tcgtattcgg cctgcaccgg gccgctcggt ggataggtac
     6721 cgctgattgg cgcgcccgcc tgaacttttt caagcacaaa agtttccaac tcttccatct
     6781 ccagcccgct tttcgcgacc tgctctgcca tatggcgcgg aatacagacc actccttcgt
     6841 cgtcaccaac catgatgtct cccggcatca ccagcacttc ggcacatccg atcggcacgt
     6901 tgatatcgac cgcgacgtgc ttgtggatgt gtgcggttgc ggccatgttt ttggcgaaaa
     6961 ccgcaaagcc gagcttttgt atggagacgt agtcacgcat ggcgccgtcg gtcacagcac
     7021 cagaagcgcc ccgggcctgc aggcgtgaca tcaaaatctc gccgccggca gccgcgctat
     7081 cgatattgcg gcagtcgagc accagcacct gtccgggaag tatttcatca atggccttgc
     7141 gttgcgggta tccgggtttg ccgatcgcct cgaacgttgc caggtcctcg cgcgccggta
     7201 cgaatcgcac ggtcaccgct tcacccgcca tgcgcagggt tggattgagc ggtttcactc
     7261 cgttcaggta agtattacga aagcccaact tgaacaactg ggaggtcaag gttgccgtag
     7321 aaatgcgccg taactgatcg agaatttgcg gagcaatcgg agcgggtttc atgatttttc
     7381 ctgcctgatc tgaggtcgta tcctacccca gaccgcattg cgggctctat gccccgctag
     7441 tcaagagagc cgccacgacc ccgacacggt tcatcgcaag ccggcttgca gcttgcccaa
     7501 ttcgatttgg ttaatggtgg gaggaagttt gatggacaaa gcttccagat aggtgcgctc
     7561 catgtcctgc gccttgcggt tgcgcatcgc gattacaaga ttgggccgga tgtcggaaag
     7621 cgcgcgagtc cccgcggcct tcttgtccaa aagcttgatc aaatgccagc cggcagccga
     7681 ttttatcggc ggactcactt cgcccttcgc catgccagac acggcgcggc ggatttcggg
     7741 gaccatctgt tcttcgctta cccaacccag ctcgccaccc ttttccgcgc tctccttgtg
     7801 cgcagaattc tcttttgcca actttgcgaa atcggttccg gacttcccca gttttgcgct
     7861 caattcgcca atcttctttt tggcttcgtc agctttggcc ttatcagtcc cctctggtgc
     7921 cggcagaaag atctgcgcaa gttggaactg ggccggggcg accaattgcg ccttgttgga
     7981 ctcgtagaat tgcttgattt cctcttcgga cggatagccg tcgcctggtt tggcgacgtt
     8041 ggcgacaaac gaacttacga tgacctggtc acgagctctc tcgatcatcg gctccaattc
     8101 cttgcgcttg tcccagcctt tctgctttgc ttcatgcagc acggctttgc gcatgagttc
     8161 gctgcgcacc agtctttcca atgccgccgg atctgtcgcc aactggcgac gggcatccgg
     8221 cgcttgcgca tcgaggatga gcatcaactc ggaactgcgc aattccacat cgcccatttt
     8281 cccgacgaga tcatcagcag ccgcgctaac gcaaattgca agccccatca gtaaaaatgg
     8341 caggcgcatg attacaggca tgggttaact ccaagtccgg atggaccggt aagacaggaa
     8401 tctgaccccg gaggtttccg cgggcttcgg ggagaatgtg acccggattc ggtatccaag
     8461 gcgccacatc tgggtcggca gccggaccag tctcgctggt ccggttcgcc gaaaatcaca
     8521 acgcaagact acttgaggtg cccagcgacc agcttggtca tctcgaacat cgatacggtt
     8581 tttttaccgc cgaagacgtc cttcagcttg tcatcggcat tgatgttgcg tcggttcttg
     8641 ctgtcctgca ggtcgttttt cttgatgtac gcccacagtt tcttggtgac ttccgtgcgc
     8701 ggcagcggag cgctgccaat tacggcggcg agcgacgcac tgggcgtcat tgccttcatg
     8761 aaagccgcat tgggcttgcg cttcacgcct gatgccttct ttttgggagc agctttcttt
     8821 gcgggtttct tggcagcctt ctttgcggtt ttttttgcag cagccttctt cggggcagct
     8881 ttctttacgg atttctttgc ggatttctta gcggtcttct tctttgcggc catcgtgatg
     8941 cctccaattg tagtttgctc gtatctcaat ttgcgttgcc ttgccagaag caaaggcacg
     9001 gcgagcagag aatgcccaga ggaaactgcg ttgtcaatgg aagaatgcac gtttctcata
     9061 gggaagcgcc gatgaattgt gaagtgcgca acatggattg ccgcactccg ctgccccagc
     9121 gccgagtgtc tttagactcg gctcgccgcg tgaatcaccg tagtcttgcg gagatatgcc
     9181 gatgccattt caacagcata tcggcggggt agcagttgca gcagacaaac cgtcgtgcct
     9241 gctgcgcggc cttggtggaa ccaaagcctg acgttaggga ccaggcatca ttactgcctg
     9301 tacggctgta ccagcggcgc tgctccagcg tcctgcgtcg gtcccgccgg aacggactgc
     9361 gccgcaggtg aattctgctt taccgcctgc tgtcccgggc tttgcttcaa cagccggatg
     9421 cgcgtgtcca ggtctacata gaaattcctg atttggaccg cggcatccgc ttgtgatttc
     9481 tgcagttcct cgattgatcc gcgcaaagag gccgaattgc ggcgcaaatc ttccacttcg
     9541 cgcatcagct gcaacagccc ttccgagttg atgagcgcac gggtttccgc aacctccttg
     9601 ctgagagccg tgatctgttg cgtcaaaatc acgtcgttga cttgggcatt tccgcttttg
     9661 gtctcgagcg cggcagcgcg ccgcgtaacg ccctgcaact tggtgtctat ctcgtttagg
     9721 cgccgggttg catcaacgga ggcggtggca ctttgttcca aggcgcgctg ctggacttgc
     9781 agcttcgcgt ccatctcggc gttccttcgc cggagttcag caaatgcagc accgttctga
     9841 tcgagttgac ggcgttgctc ctcacgcgcc gctaccacgc tggcaagcaa ttcctttgac
     9901 gtggcctggg cagtgtccgc gcgtgcctgc gtttcgacta cggaagcttc cagggcatgc
     9961 attgattgct gggattgcga tagctccgca cgcacttcct ttatcaggct tgcgttggag
    10021 tcctggcctg cacgcagcga tttgactgcg taaccctgtt cggccgtttg ggcttccgtc
    10081 tgacgcaatt gatccggagt tgcgcaaccg gcgagtgcca ggacaacata cacataggcg
    10141 ataagctgct tcatgtgctt tgcccgccta atagacaatg tctacgcgac gattcttgct
    10201 atagctttct tcatctttgc cgttcgcgat cggtttttca gcgccgaaac tgacgacttg
    10261 aatgcgcccg ccatccgcgc cggccagttc cagcgcgcgt ttgaccgctt gagcgcgcct
    10321 cgcaccaaga gcgaggttgt actcccggct gccacgctcg tcgcaatttc cctggacttc
    10381 aaaacttacg cgcgggctag ccttcagata agcagcgttc tgcaacaaca tggtgtcgta
    10441 gtgttttggg atggtggact ggtcatactc gaagaaaatg ctgcgcgcat cggccgccgg
    10501 agctggagcc aggctttcaa ccgcggcttg cccgctcttc tccccggccg gaaatatcgc
    10561 tatgtcgtca tcccggtgag cggctgccgt ctcgggtttg ccaacctgct ctggtgtcac
    10621 agccgccggt gcagcaacct tgccgcccgg cgaactgccg ttcgggtccc cgccctgcgc
    10681 ttgttgctgt tccctctgcg ctgcttcagt ttcctgcgtt gtgcgaagct tgctgatttc
    10741 cgcctcttgt tcggcaagct gccgtttcaa ttgcgcttcc tcggcaccgg cgtttgcgga
    10801 tgcggttttc ggctggccgg taccgccggc cgctgaggac tccgtactcg aggtggccgg
    10861 ctttcctgct gggggcagcg cacttcccgg gttatccgca ctcggttgca atgactttgt
    10921 tgccgcatcc gcggtggcgc cttcctgggt accgaccccc gcctctgata ttggcgcggc
    10981 agtctggcct cctgcaccac tgctgcttct ttgtgtcgtc gcacagctcg ccaaagcagt
    11041 gcaaacaaga atcatgaaaa tatttctggt aatgctgaac aatgatgcat cgaccatcta
    11101 tctaccctcc tagggctcaa gcagtaaaag tttcatccac caccagaatg ctcactacaa
    11161 attgtttcac ccatcgcatt tgcatgcaat gtaaaaagca tggttttgtg cttgcataaa
    11221 tggtccgccg gtcgacgcgc ttaaccagcg tagttggaag aagaggaata ctgcaggcct
    11281 cgcagggcgg atactttcgc cgccagcggc gctagtggtt ttgcctttgc acatagacaa
    11341 ccgcatcgag cgcgatgcag ccttctccct ctgcgccgat gagaatcgga ttcaaatcca
    11401 aattagctat gccggcctgc cggtccgtca ttagtcggct tactgaaagc accgcactgc
    11461 agaatgcgtc gacatcgagc gctgcctccc cgcgcacacc ggcaagcaga ggagcaatac
    11521 gcaggcgcgc gaatgcctgt ttgacttccg cagcgcaaaa aggcgcaatc agcaactgga
    11581 tgtccggcat ggcttccaca tatttgccgc catcccccac cagaacgacc gggccgaaca
    11641 ccggatcgac tcgcgcaccg atcatcagct cgcggcggcc ctgggccatc tgcgcaacga
    11701 tgacgccgtc gaaacgagcc ccgtgcttca aaagagcctt ctttatttct gtgaatgcgg
    11761 ctcgcacttg accggcttgg tcggctccca ggtgtaccag ccccaattcg cttttgtgcg
    11821 cgacatcggc ggaacagcct ttcagagcta cccggccgcc taattgtgca agcgcctgta
    11881 ccgcctcttc tgcgttgtgg cacaacctga atttcacgac tggcacgcct gcagtcgcga
    11941 gcaaggtaag actctcggct tcgtttagca ttgtggccgg ccccgtcgga ggagcgtatc
    12001 gctgagggga cgccgctggt ttccaagctt gcatcagctc tccgtgcgac agaaactggt
    12061 ttagcgccgc aaccgcttcg tattcagtcg gaaacacggg taacccatac gccttgaagt
    12121 gagccgcaat gttggactgc gggattgcag cgaccagcgg cttgccagtc gcttttgcga
    12181 aattcgccga atcgcgggcg aaagcatcaa cgtcgtaacc ctgccccgcc accggaatgc
    12241 caatcaggaa agcatctgcg gcggaatctc gcgcaagtac gggcaggatc tgagagaaca
    12301 gcccgctgtt gctgagcagg gcagctgtaa tatcgattgg attggttgtg gtggcgaatc
    12361 ccgggagaat cttgccgaga ccggcacgcg tctcatcagc aagcttggcc agtggcatgc
    12421 cgagcgtgct ggcggcgtcg gctgccatga cgcaaaccgc tccggaattg ctgattgcca
    12481 ccagcctgcg gcctttcggc ttccagcctt tcaagtacat ctgcgcactt gccaccagtt
    12541 cggcggtgtc gcgggcgcgc catatcccct gcttggcgag gaaggcgtcc actgttctgt
    12601 cctcgttcgc caacgcgccg gtgtgggact ttgcggcttc ctggccagcg agggtgcggc
    12661 ccgacttcaa cgcaatgatg ggcagattgc gtgcgtgtgc gatgcgggcc gtctcggcca
    12721 ggaagaatga atcgtcaatg ctttcgagat aaagcagcaa cagcttcaga tcggggtcct
    12781 cggccaccac ggacgccagt tccgacacag taacgtctgc gtcattgcca gtggcgtgtg
    12841 aatggcgtac accgagtccc tgcgcccgca gcaaaccgta cggcacgacg ctcatcgcac
    12901 ccgactggct gaccacggcg atcgggccgt ccagcggcgg cgcttcgatg tacatggtcg
    12961 aaaacgacaa gatggcgccg gtgccgaagt tagccagtcc ttgagaattc ggaccaacga
    13021 cccgcatgcc tgcagctcgc gctgcgtcac gcatgcgcgc ttcttttgcc tttccggcaa
    13081 ctgcatctgt ttcaccgaag ccggaactca tcactaccgc agtctttacc ccgagttgtg
    13141 cgcactgttc gaccgctgcg atcgcggcat cgccggccag cgctacgatt gcggcctctg
    13201 gcacgtcggg taaatcggcc agcgaaggat aggctttgaa gccctggatt tcggcacgct
    13261 tgggatttat tggataaacc ctacccttga agccgtggcg ggcaagatag agcagtgggc
    13321 gtccgcctat cttgttttcg ttttccgacg caccaatgac tgcgacggac ttcgggttca
    13381 gtacggtgcg caaggcgtcg cgcaggacgt cgttcatatc acggcctcca gatcaattta
    13441 cgcttgttgt tcctggcccg gcggactcca cgacgcgcta cccgcaaccg ggcgccaggg
    13501 cgctaacgtg tccgcgcgtc ccgcctatct tgttttcgtt ttccgacgca ccaatgactg
    13561 cgacggactt cgggttcagt acggtgcgca aggcgtcgcg caggacgtcg ttca
//
LOCUS       DATMZQ010000401         4193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3080017, whole genome shotgun sequence.
ACCESSION   DATMZQ010000401 DATMZQ010000000
VERSION     DATMZQ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080017"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1014
                     /locus_tag="VN496_12865"
     CDS             154..1014
                     /locus_tag="VN496_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HXF17892.1"
                     /translation="MRNLNEANLTDAVLAKLEGAKDPRFKQIMESLVRHLHAFIRETE
                     LTEQEWMTGIQFLTATGQRCDDKRQEYILLSDTLGVSMLVDAINHRKPGDATETTVLG
                     PFYVNGAKAMPMWADIAAGAPGDPAYVSGRVVDVNNRPIAGAMLDVWQTDGEGVYDVQ
                     RPGGNEHYARGRFTTGADGRYGFKTVKPVSYPIPTDGPVGKMLLGMGRHPYRPAHVHV
                     IASAPGYERVATHLFVEGDAYLDSDAVFGVKHSLVVNFKAHPAGPAPDGKKSNLPYSS
                     AEFDFKLVPA"
     gene            1014..1379
                     /locus_tag="VN496_12870"
     CDS             1014..1379
                     /locus_tag="VN496_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXF17893.1"
                     /translation="MQEWRTWPFAPEPGTLLCRLPEIPDNGAREVVFGEGYDCFRVLL
                     LRSGARVSAYRNRCAHVHIPLNYEEQLFHVLDGEVLMCAHHGAMYRIADGVCFDGPCE
                     GASLTPIPIAIRGDDIVLG"
     gene            complement(1439..2701)
                     /locus_tag="VN496_12875"
     CDS             complement(1439..2701)
                     /locus_tag="VN496_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17894.1"
                     /translation="MTFKRITAGHGVLAMLCMMYLITYVDRVNVSTAAAAFKGELGLS
                     NMQVGFVFSAFAYPYLVFQVVGGWFGDRFGARRTLFVCGVVWASATILTGFASGLVSL
                     LAARVLLGLGEGATFPAATRAMSNWSAPSQRGFAQGITHAFARIGNAITPPIVAWLMV
                     AMTWRGSFFVLGAISMVWAIVWIFYFRDDPKDHPRITQGEIDKLPPYSGNRAGRPVPW
                     SRLARRMAPVTLVYFCYGWTLWLFLSWIPIYFLQNYKMDLKHSALFASGVFFAGVFGD
                     TAGGLLSDWLLRKTGRRQIARRNLVVFGMSGAMLSLLPLLLTQDVVVAAACLSLGFFF
                     AELTIGPMWAIPMDIAPQYSGVASGLMNVGSAFAAIVSPVVAGLVIDMTGNWTLPFIG
                     SMVLMALGAVLAFWMHPEQPLVMEDNAP"
     gene            complement(2730..3617)
                     /locus_tag="VN496_12880"
     CDS             complement(2730..3617)
                     /locus_tag="VN496_12880"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF17895.1"
                     /translation="MSIEKPRVGCIGAGVLGAAIMRRLIDCGFAPAVWNRDRAKLSSL
                     VAAGAKEAGSAQDIARDNVFVITCVSDGAALEQVVFGARGIAAGGAPGKVLVDMSTCA
                     AAHTQDMAKRLTAQCGMAWLDAPISGGAPAALEGRMAIMVGGQPDIFERARPLWDALS
                     ARATLMGPNGAGQSTKMINQVLVSTGLAVLAEACAFAERAGIDAAKIPQALAGGRADS
                     RQLQEMFPKMVKSDFAITGRVSLMLKDLELIHDLARHVGAPMPVTAGVTESFRKMVGD
                     GLGDRDNTELVNFYRGRTP"
     gene            complement(3639..>4193)
                     /locus_tag="VN496_12885"
     CDS             complement(3639..>4193)
                     /locus_tag="VN496_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXF17896.1"
                     /translation="AGSVLARGIADGPDEDFEALPLTMLGALSQATRSIRIVTPYFLP
                     DPTLIDALRVAALRGVRVEVLLPARGNLRFVEWAATAQLAQIVRWGCRVYLSPPPFDH
                     SKLFLVDGLWCLIGSANWDPRSLRLNFEYVVECYSEELVSRITQVSDAKIAAAQLLTI
                     EALESRSLPVKLRDGIVRLAQPYL"
BASE COUNT          922 a         1290 c         1242 g          739 t
ORIGIN      
        1 ctgctcgatg cgtggctgac cgacaccctg gccgtcgcac ccaaggcgca aatgatttac
       61 ttccaggacg acccgaaagc gcgtgcgaaa ctgattcgtt ttatcgagtc gctgaagtga
      121 tttgatcaag aagcgtgatc cggagattca cccatgcgta acctgaacga agcgaacctc
      181 accgacgcgg tactggcaaa attagaaggc gcaaaagatc cgcgcttcaa gcagatcatg
      241 gaaagcctgg tccggcactt gcacgcgttt attcgcgaaa ccgagttgac ggaacaggaa
      301 tggatgaccg gaatccagtt tctcaccgca accggtcagc gctgcgacga caagcgccag
      361 gagtacatcc tgctgtcaga cacgctcggc gtatcgatgc tggtggacgc aatcaatcac
      421 cgcaagccgg gcgatgccac ggaaactacg gtgctggggc cgttctatgt aaacggcgcg
      481 aaagccatgc cgatgtgggc agacatcgcc gccggcgctc ccggcgaccc ggcgtatgta
      541 tccggccgcg ttgtcgacgt caacaatcgg ccgattgccg gcgcaatgct cgatgtatgg
      601 caaactgacg gcgaaggtgt ctacgacgtt cagcggccag gcggcaacga acattacgcg
      661 cgtggccgtt ttaccactgg agcggacggc cgctatggtt tcaaaaccgt gaagccggtg
      721 agctatccca ttccgactga cggcccggtc ggcaaaatgc tgctcggaat ggggcgccat
      781 ccgtatcggc cggctcatgt gcatgtcatc gcgagcgcac cgggatacga gcgcgtggcg
      841 acccacctgt tcgtcgaagg cgatgcgtac ctggattcgg acgcggtgtt cggggtcaaa
      901 cattcgctgg tagtcaactt caaagcgcat ccagcaggcc ccgcgccgga cgggaaaaaa
      961 tccaacctgc cgtactcgag cgcagagttt gatttcaagc tggtcccggc ttgatgcagg
     1021 agtggcgtac ctggccgttc gcgccggaac ccggcacgct actctgcagg ttgcccgaga
     1081 tcccggataa cggtgcaaga gaagtggtgt tcggcgaggg ctatgactgc tttcgcgtat
     1141 tgctgctgcg ctccggcgcc cgcgtcagtg cctatcgcaa ccgttgtgca cacgttcata
     1201 ttccgctgaa ctacgaagag caattgttcc atgtgctcga tggagaagtg ctgatgtgcg
     1261 cgcatcatgg cgcgatgtac cgcatcgccg acggggtctg cttcgatggc ccgtgcgaag
     1321 gcgcgagcct tacgccgatt ccgattgcaa tcaggggcga tgacatcgtg cttggctgat
     1381 gctgctctac gcgttagcgc atcaggtatg ccggcgactt cacttcggac cgtgtgggct
     1441 agggtgcatt gtcttccatg acaagcggct gctcaggatg catccagaat gcaagcacgg
     1501 cacccagtgc catcagaacc atcgaaccga tgaacggcaa cgtccagttg cccgtcatgt
     1561 cgataaccaa acctgcgacc actggagaaa caatggctgc aaaagcggag ccgacattca
     1621 tcaagccact ggcaacgcct gaatactgcg gagcgatgtc catcggaatc gcccacatcg
     1681 gtccaatggt gagctccgcg aaaaagaagc cgaggctcag gcatgccgca gcgacaacga
     1741 catcctgcgt aagcagcaga ggcaataacg acagcatggc gccggacatg ccgaacacca
     1801 cgagattgcg gcgtgcgatc tgccggcgcc ccgtcttgcg aagcagccag tcgctcaaga
     1861 gcccgcctgc cgtgtcgccg aacacgccgg cgaaaaagac gcctgaagca aacaatgccg
     1921 aatgcttcag gtccatcttg tagttctgca ggaaatagat cgggatccag ctcagaaaca
     1981 accacagcgt ccagccgtaa cagaaataca ccagggtcac cggcgccatg cggcgggcaa
     2041 gacgcgacca gggcaccggg cgacccgcgc ggttgcccga gtacggcgga agcttgtcga
     2101 tctcaccttg cgtgatgcgt ggatggtcct tgggatcgtc gcggaaatag aatatccaga
     2161 caatcgccca caccatgctg atcgcgccaa gcacaaaaaa agagccgcgc caggtcatcg
     2221 caaccattag ccaggcaacg atgggtggcg tgattgcatt gccgatgcgc gcgaacgcat
     2281 gtgtgatgcc ctgcgcgaaa ccacgttgtg atggcgcgct ccagttcgac atggcacgcg
     2341 tcgcggccgg aaatgtggcg ccttcgccaa gacccaacag cacgcgcgcc gcgagcagcg
     2401 acaccagtcc gcttgcgaat ccggtcaaga tcgtggcgct cgcccaaact acgccgcaca
     2461 cgaacaaggt gcgccgggca ccgaagcggt cgccgaacca gccgccaacg acctggaaca
     2521 ccagataggg atacgcaaag gcggaaaaca cgaatccgac ctgcatgttg ctcaagccga
     2581 gttcgccttt gaacgccgcc gcagcagtgc tgacgttaac acggtcgacg tacgtgatga
     2641 ggtacatcat gcacagcatg gcgagcacgc cgtggccggc cgtgattctt ttgaaagtca
     2701 tggcatcgcg cttgaaccag cctggaattt cagggcgtcc tgccgcgata aaaattaacc
     2761 agttcggtat tgtcgcgatc gccgagtcca tcgcccacca tcttgcgaaa cgactccgta
     2821 acgccagcgg tgacaggcat gggagcgccg acgtggcggg caagatcgtg gatcagttcc
     2881 agatctttca gcattagcga cacgcgcccc gtgatcgcga aatccgactt caccatcttt
     2941 ggaaacattt cctgcaactg cctggagtcc gcgcgcccgc ctgcaagtgc ctgtggaatt
     3001 tttgcagcat cgatgccggc acgctcggca aacgcgcacg cctccgcgag cacggcaagg
     3061 ccggtggaaa ccagcacctg gttgatcatc ttggtggatt gtcctgcgcc gttcggtccc
     3121 atcagcgtgg ctcgcgctga cagtgcatcc cacagcggac gtgcacgctc gaagatatcc
     3181 ggctggccgc cgaccatgat tgccatgcgt ccctcaagag ccgctggcgc acctccggaa
     3241 atcggggcgt ccagccaggc catgccgcat tgcgcagtca ggcgttttgc catgtcctgc
     3301 gtgtgcgctg cggcgcacgt tgacatgtcg acgagcacct tgccgggcgc acctcccgcg
     3361 gcgattccgc gcgcgccgaa caccacttgt tccagcgcgg caccgtcact gacgcacgtg
     3421 atgacgaaca cattgtcccg cgcaatatcc tgcgcgctgc cggcttcctt tgctccggca
     3481 gcaaccaggg aagacagctt agcccggtcg cgattccata cggcgggcgc aaagccgcaa
     3541 tcgatgaggc gccgcatgat cgcagcaccc agaacgccgg ctccaatgca gccgacgcgc
     3601 ggtttttcga tgctcattgt ttttctcctg agtatgcctt aaagataggg ctgcgccagc
     3661 cgaacgatgc catcgcgcaa tttgacgggc aggctcctgc tttccagagc ctctatggtc
     3721 agtaattgcg cggcggcgat cttcgcatcc gacacttgag tgatgcggga aaccagttcc
     3781 tccgagtagc actcgaccac gtactcgaaa ttcaggcgca ggctgcgtgg atcccagttt
     3841 gccgagccga tgaggcacca taggccgtcc accagaaata atttggaatg gtcgaaaggc
     3901 ggaggcgaca aatatacccg gcaaccccag cggacaatct gcgcgagttg cgccgtggcg
     3961 gcccattcga cgaagcgcag attgccgcgc gccggcaaca agacctcaac acgcacgccg
     4021 cgcaatgccg caacacgcag tgcatcgatc agcgtcggat caggcaggaa gtagggcgtc
     4081 acgatgcgaa tcgagcgcgt tgcctgggac agggcgccca gcatggtcag cggcaacgct
     4141 tcgaaatctt catcggggcc atcggcgatg ccgcgcgcga gcacagaacc ggc
//
LOCUS       DATMZQ010000402         5226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3081101, whole genome shotgun sequence.
ACCESSION   DATMZQ010000402 DATMZQ010000000
VERSION     DATMZQ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3081101"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VN496_12890"
     CDS             <1..804
                     /locus_tag="VN496_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXF17897.1"
                     /translation="RAEAEVEKALAEYRFDHVAGVIYKFVWDEYCDWYVEIAKVQMLA
                     GTEAQQRASRRTLLRVLEATLRLAHPIIPFITEELWQRVAPVAGRHPGGEASIMVQPY
                     PAWQPGRIDEQAEAWIGRLKSLVEACRTLRGEMGLSPAQRIPLLATGDADSLVAFFPY
                     LQALAKLSHTRIVDTLPDTEAPIAVVGQTHLMLEIKVDVAAEIARIEKEVARLESEIT
                     KARNKLSNAGFTERAPAAVVAQERDRLVAFEDTVAKLQDQVGRLKRKAA"
     gene            831..2072
                     /locus_tag="VN496_12895"
     CDS             831..2072
                     /locus_tag="VN496_12895"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17898.1"
                     /translation="MPALRAFRHRNFRLFYGGQAISLVGTWIQQIALSWLVYRTTGSA
                     FLLGLVTFCAQIPMLIFLPLAGLISDRHDRRKVMVFAYVLASLQALTLAVLTLRDAIR
                     VWHILLLGFSYGVIMAFETPARQSLISQMVASRDDLPNAIALNSVLMNSGRLIGPSIA
                     GLLLVFIGEGWCFLINAASFMAIVASVLMMELAPRHASTLRGSMAQDLAIAARYAWGT
                     APIRLFLVLVALISLTASPYTVLMPIFARDIFGGDAHTLGFLVGSAGLGAVIGTIFLS
                     TRPHIYGLSRLVPFTSASAGIALMLVGMSTNYLLSLAFMAILGFGIIVTAASVNMMLQ
                     SLVDEDKRGRIVSFYAMAFLGMAPVGGLMAGSLASQIGAPATAMVNGTCCLLGTLVLM
                     RARPAVWDDLHSLLSSVQKNR"
     gene            complement(2182..3321)
                     /locus_tag="VN496_12900"
     CDS             complement(2182..3321)
                     /locus_tag="VN496_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXF17899.1"
                     /translation="MAVASAFIAPAAMADVTISGAINVGIEVGKSSAGSAPGAQSLTQ
                     TSLGATYSHVDLESVDDIGGGNKVIFHYQMNLAGDSPGAMGNTGGITNRNSFLGIAGG
                     WGAFKMGVNENVYERMMYTADPLDGAAGPAGNLNILGTPGGGFIFENNVNNCTATGCP
                     DFYRRTQQTIWYESPKFAGFDFEVDYTLSAFKNQNQNPQIFSIGGKWQPEGAPFYIDA
                     AYERHNDLFGTVAIGDTVIATSTSKDTGVQVGGGWQLGDLGLHLRYEQLKYETTGGLG
                     GDNEYKRNAYWFAVKYNLPSGYIGAEIGIADDGKLNGSDVSDTGARMFGIGYFHNLSK
                     QSQLQFIYGRMDNKDQATYGFAGTPNGAAPGSDHDVLHVGIKHTF"
     gene            complement(3617..4450)
                     /gene="trpC"
                     /locus_tag="VN496_12905"
     CDS             complement(3617..4450)
                     /gene="trpC"
                     /locus_tag="VN496_12905"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXF17900.1"
                     /translation="MSDILNKIINIKHDEIAAAKRKRALTSLRADAENAAVPRDFLGA
                     LRERIAADIPAVIAEIKKASPSKGVLRADFDPVAIAKSYARYGAACLSVLTDVQFFQG
                     KPEYLRQARDACELPVLRKDFMIDPYQIYESRTLGADAILLIVAALDDDAMMALEQVA
                     LDLRMAVLVEVHDARELERALRLRTPLLGINNRNLRTFETHLETTLDLVASIPPDKLV
                     VTESGILSSRDVDLMRAAGVGAFLVGEAFMRANDPGEALSTLFRFAPVEHGRVNPEKI
                     P"
     gene            complement(4456..>5226)
                     /gene="trpD"
                     /locus_tag="VN496_12910"
     CDS             complement(4456..>5226)
                     /gene="trpD"
                     /locus_tag="VN496_12910"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXF17901.1"
                     /translation="SAHTYNISTAAVFVAAAAGAKVAKHGGRSVSSKSGSADILEALG
                     VNLNLAPDQVAQCIQSTGVGFMFAPNHHSAMKHAAPVRRELGVRTLFNILGPLTNPAG
                     ARNQLIGVFHPDLVGILARVLQRLGSRHVMVVHGTEGLDEISISGDTLIGELKDDQIL
                     EYKVNPQQFGIPPGNLSDIQVRDSTASKEMLVSALSNEPGPARDILALNAGAAIYVAG
                     LADTHEAGVQKALGVLAAGGAKEKLDEFVTFTRKLKSG"
BASE COUNT         1080 a         1469 c         1456 g         1221 t
ORIGIN      
        1 agagccgagg cggaagtgga gaaagcattg gcggaatacc gcttcgacca cgtcgccggt
       61 gtgatttata agtttgtctg ggacgaatac tgtgattggt acgtcgaaat cgcaaaagtg
      121 caaatgctgg ctggcaccga agctcaacag cgcgcttccc gacggacttt actacgcgta
      181 ctggaagcaa cactacgcct ggcacatccc atcatcccgt tcatcaccga ggaactatgg
      241 caaagagtgg cccctgttgc tggtcgtcat cccggcgggg aagcctcaat catggtgcag
      301 ccatatcccg cctggcagcc cgggcgaatt gacgaacagg cagaagcgtg gattgggcgg
      361 ctcaaatcgc ttgtggaagc atgccggacg ctgcgtggtg aaatgggtct ttcgccggcg
      421 cagcgcatac cgctgctggc cactggtgac gccgattctc tggtcgcttt ttttccttac
      481 ctgcaagctc tggcgaaact ctcccatacc agaatcgttg acacacttcc ggatacagag
      541 gccccaatag ctgtggttgg gcaaacgcac ctgatgcttg aaattaaggt cgacgttgct
      601 gcggaaatcg cgcgcattga aaaagaagtc gcgagattgg aaagcgagat caccaaagcg
      661 agaaacaagc tatccaatgc cggtttcaca gagcgcgcac ctgccgcggt ggtggcgcag
      721 gaacgggacc ggcttgtggc tttcgaagat acggtagcga aactccagga tcaagtgggc
      781 aggctgaaac gtaaggcggc ttgactgtcg tgcattggcg cttggcacgc gtgcctgcac
      841 tgcgagcgtt ccggcatcgc aacttcaggc tattctacgg cggccaagcc atctcgctgg
      901 tcggaacctg gatacaacag atcgcattgt cctggctggt gtatcgcacc accggctccg
      961 ccttcctgct cgggctggtt acgttctgcg cccaaatacc gatgctcatt ttcctgccgc
     1021 tggctggact gatcagcgat cggcatgatc gtcgcaaggt aatggtgttt gcctatgtac
     1081 ttgcgtcact gcaagcattg accttggcag tgctcacact gagggacgca atccgtgtgt
     1141 ggcacatttt gctgctgggc ttttcgtatg gcgtcatcat ggcctttgag acaccagcgc
     1201 ggcaatcttt gatcagccag atggttgctt cgcgggatga tctgccaaac gccatcgcgt
     1261 tgaattcagt gctgatgaac agcggtcgtt taatcgggcc gtcaattgcc gggttgttgc
     1321 tcgtgttcat cggcgaaggc tggtgtttcc tgatcaacgc cgccagtttc atggcaatag
     1381 ttgcttcggt cctcatgatg gagttggcgc caaggcacgc gtccacctta cgtggctcaa
     1441 tggctcagga tcttgccatc gcggcgcgct atgcctgggg caccgcgccg atccggcttt
     1501 ttttggttct ggttgccctg atcagtttga ccgcctctcc ttacactgtg cttatgccga
     1561 tcttcgcgcg agatattttt ggtggcgatg cccatacgtt gggctttctg gtcggcagcg
     1621 cgggattggg cgcggtcatc ggaaccattt ttctaagtac gcgcccacac atttacggac
     1681 taagcaggct ggttccattt acttctgcgt cggccggcat tgcactcatg ttggtcggga
     1741 tgtcgacgaa ctacttgctg tcgttggcgt tcatggccat tctcggattt ggcatcatcg
     1801 tcacggctgc ctcggtgaat atgatgctgc agagcctggt agatgaagac aaacgcggac
     1861 gcattgtgag cttctacgcc atggctttcc tgggcatggc gccagtgggc ggtttgatgg
     1921 ctgggtcgct cgccagccaa attggcgctc cagccacggc catggtcaat ggcacttgct
     1981 gcttgctggg tacgttggtt ctaatgcggg cgcgccctgc ggtttgggac gatctgcaca
     2041 gcttgctgtc cagcgtccag aaaaaccgct gaccagcgtt tgccatcgtt taggctcacg
     2101 ccacagctct agttttcggc aataaaaaac gggcgcccga aggcgcccgt tgcattgcat
     2161 aacagcttgg gtcggtccag tttagaacgt gtgcttgata ccaacgtgca acacgtcgtg
     2221 gtcggaacca ggagcggcgc catttggtgt gcccgcaaat ccataggtag cctggtcttt
     2281 gttatccatg cgcccataga tgaactgcaa ttgcgattgt ttggacaggt tatgaaagta
     2341 gccgatacca aacatcctgg cgccggtatc gctcacgtcg ctgccattga gcttcccatc
     2401 gtctgcaata ccaatttcag caccgatata gccggacgga aggttgtact tgactgcgaa
     2461 ccagtaagca ttgcgcttgt attcattgtc accgcccagg cccccggttg tttcgtactt
     2521 cagctgttca tagcgcaggt gcaagcccaa gtcaccaagt tgccagccgc caccgacttg
     2581 aacacccgtg tccttggatg tggaagtcgc gatgacggtg tcgccgattg caacggtacc
     2641 aaacaggtcg ttatgccgtt cgtaggcggc gtcgatgtag aacggagcgc cttccggctg
     2701 ccacttgccg ccgatggaga aaatctgcgg gttctggttc tggttcttga acgcgctgag
     2761 cgtgtaatca acttcgaagt caaagccggc aaacttcggg ctttcgtacc aaatggtctg
     2821 ttgcgtacgg cgatagaaat ccgggcagcc ggttgcggta cagttattta cgttattctc
     2881 gaaaataaac ccgccgccag gagtaccgag gatgttcagg ttaccggctg gacctgctgc
     2941 accgtccaac ggatccgccg tgtacatcat gcgctcatac acgttttcat tgacacccat
     3001 cttgaacgcg ccccaaccgc cggcaatacc caagaagctg ttgcggttag tgatcccgcc
     3061 cgtattgccc atggctcctg gagaatcacc cgccaagttc atctgataat gaaaaatcac
     3121 tttgttgccg ccgccgatat catctaccga ctctaggtcc acgtgcgagt atgtggcgcc
     3181 gagtgaagtt tgggtcaggc tctgtgctcc aggcgctgaa cctgcacttg acttaccaac
     3241 ctctatacca acattgatag caccgctaat ggtgacatcc gccatcgcgg ccggtgcgat
     3301 aaacgcgctc gctaccgcca ctgcaattag actcttcttc atgcgatctc cttttaatgg
     3361 ggcataagcc agtcgggcgc gttaactcgc ccttttgcgg cgccatgttt ttggcgctgc
     3421 ggattcaacc tctcccggga aagaaagaag ttgccgcgat tctgccaaaa cgcaacagct
     3481 cgcggcaagg aattcccctc tgagatagcc cgtttttgtg gcttttgttg tgcgattgac
     3541 acactgcggt gcacaaaaag cggcttttga gtgctgaagc tctagctaaa tgcttacagc
     3601 tacttatcgc gcgaagctaa ggaattttct ccggattgac ccgaccatgc tcgactggcg
     3661 caaatcgaaa cagagtcgat agcgcttcac caggatcgtt tgctcgcata aaagcctcgc
     3721 ccaccagaaa agcgccaacg cctgctgcgc gcatgagatc gacatcgcgc gatgaaagaa
     3781 tgccgctttc ggtaacgacc agcttgtctg gcgggatgct cgctaccaaa tcgagtgtcg
     3841 tttcgagatg ggtttcgaaa gtgcgcaagt tgcgattgtt gatacccagc aacggcgtgc
     3901 gaagccgcag agcgcgttcc aactcgcgcg catcgtgcac ttccacgagg accgccatgc
     3961 ggaggtccag cgcgacctgt tccagtgcca tcatggcgtc atcgtccagt gcggcgacga
     4021 tcagcaagat tgcatccgca cccaaagttc gagattcata aatctggtac ggatcaatca
     4081 tgaaatcctt tcgcagtacc ggcagttcac aggcgtcgcg cgcttggcgc aaatattctg
     4141 gtttgccttg gaagaactgc acatccgtga ggactgacag gcaagcggcg ccgtatcgcg
     4201 cataggactt cgcaatcgct acgggatcga aatcggcgcg cagaacgccc ttgctcggac
     4261 tggccttttt gatttcggca atgaccgccg gtatatcggc cgcaatccgc tcgcgcagtg
     4321 cgcccagaaa atcacggggt acggccgcat tttccgcgtc ggctcgcagc gatgtaaggg
     4381 cgcgtttgcg cttggctgca gcgatttcgt catgttttat gttaattatt ttgttcagga
     4441 tgtcggacat ggcagtcaac ccgacttgag tttccgggtg aacgtgacga attcatcgag
     4501 cttttccttg gcgccgccgg ctgcaagcac accaagcgcc ttttgcactc cagcttcatg
     4561 cgtgtcagcc agcccggcga cataaatcgc cgctccagca ttcagtgcca ggatatcgcg
     4621 cgccggacca ggctcgttgg aaagcgcaga caccagcatt tcctttgatg cggtgctatc
     4681 gcggacctgg atgtcggaca agttgcccgg tgggattcca aattgctgcg gatttacttt
     4741 gtactcgagt atctgatcat ccttcagttc gccaatcagg gtatcgccgg aaattgaaat
     4801 ctcgtccaat ccttcggtgc cgtgcaccac catgacatgc ctgctgccca gtcgctggag
     4861 cacccgcgcc aggatgccga ccagatcagg gtggaatacc ccgatcaact gattacgggc
     4921 ccccgcggga ttagtcagcg ggccgaggat attgaacagc gtacgaacgc ctaattcacg
     4981 gcgcaccggt gcggcatgtt tcatcgcgct gtggtgattg ggcgcaaaca tgaatcccac
     5041 gcccgtactt tgtatgcact gagccacctg atccggcgca agattcaaat tcaccccgag
     5101 tgcctcgaga atatcggcac taccggattt gctggatacg gatcgccccc catgcttggc
     5161 gacttttgcg ccagccgcag cggcaacgaa cacggccgcc gtcgaaatgt tgtaagtgtg
     5221 tgcgct
//
LOCUS       DATMZQ010000403         3894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3081852, whole genome shotgun sequence.
ACCESSION   DATMZQ010000403 DATMZQ010000000
VERSION     DATMZQ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3081852"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3845)
                     /locus_tag="VN496_12915"
     CDS             complement(<1..3845)
                     /locus_tag="VN496_12915"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXF17902.1"
                     /translation="MRCTFKLRSDVVSLAGDTLAGDQTFEFNTGGPAILEMLPFEGNN
                     IDEEQIFLLGLDAPATDQSVLENAYCDVRGVTERIPVRVIGGEERKALLDSRRDFVDR
                     WLRLLFKDGREIIMDGRPFGRGSRLDQFLNGDAGAKLPLVALQCQRRLPNNGQVSLVW
                     GKGIMSDSGIPTGQDQSLAYKVRDTFQARFTCDRVNKDAQCLPILPMRLAFSAPIPRA
                     TAQMIVLKVQGKTIAAGLPDANKAPEFVDEVFFSGPFPERSQFALDLPPDVTDDAGRK
                     LANQKRFPLLVRTDEYPPLAKFAARFGIIEAKGDGMMPVTLRNLDAVLEARMAHTGRQ
                     GDAAGNVTEKADAAINWLKNKLDEAKQSDAGVPGSYSRVADGDVMAMLEWMKRLRKME
                     NERWRYDQKTNKDVLQYRLGQNSIFAEGDRVTRLQVPRPDGARSYEVVGIPLKKPGFY
                     VVELASPRLGAALLNTRAPYYVQSAALVTNLAVHFKWGRENALVWVTALDSGVPVPKA
                     QVAIQDCSGRVFFRGQTDVLGRARVTAALPVRESLPACVHEYDKQLIVSARLADDLSF
                     VMSEWNEGIARWRFNLRQGGWNGPYLVTTVFDRTLLRAGETVNMKHYYRRHEAKGFSF
                     VPADNLPKSITIMHQGSAQKYEIPVKWDTRNIAESSWQIPAGAKKGTYLVQMTDTLDV
                     RPGARGTTRSAGSFRVEDFRVPLMKAEIFGPKAMPVNAQSIDVDVHLRYLAGGMAAYA
                     PVKVRAVVRPKNVAFADYDGFVFANGKVVEGPEQPSGAQWFSGEYELGDADTDEPIPA
                     QPVNGVRPLKTVELALDSAGAARVRLPGLPVADTPQEVNAELEYADPNGEVLTASTRV
                     PLWPAKLLLGIKPDAWTLSKDELKFQVLALDLTGKPIKNQQVDVQLYARTLFSHRKRL
                     IGGFYGYESGAEVNKVDSTCQGKTDDKGRLFCAVKPPVSGNIVMGAVAKDREGHLAYA
                     NASAWVAGTQAWWFDQSNDDRIDLIPERKHYEPGQTAQFQVRMPFRQATALITVEREG
                     VIDSFVQPLSGEAPVIKVPLKGNYAPNVFVSALVVRGRVSDVQPTALVDLGKPAFKMG
                     VAEINVGWRAHELTVKVSADKETYKVREKARVSVQVTQRAGGKPPRAGEVALVAVDEG
                     LLELLPNDSWKLLEAMMQPRGIEVDTSTAAMQVVGKRHYGRKALVQGGGGGHQAAREL
                     FDTLLLWKGRVALDDKGRARVEIPLNDSLTSFRIIAVADAGLGLFGTGQTSIRATQDL
                     MLMSGLPPLVREGDRFDAR"
BASE COUNT          732 a         1253 c         1103 g          806 t
ORIGIN      
        1 aagcgcgcat cgaagcgatc gccttctcgc accagcggcg gcaggcccga catcagcatc
       61 aggtcctgcg tggcccggat ggaggtctgg ccggtgccga acaatcccag acccgcatcc
      121 gccacggcaa tgatgcgaaa cgaggtcagt gaatcgttca gcggaatttc gacacgcgcc
      181 cggcccttgt cgtcgagcgc gaccctgcct ttccacagca gcaacgtgtc gaacagttcg
      241 cgcgccgcct gatgaccgcc gccgcctcct tgtaccagcg ccttgcggcc atagtggcgc
      301 ttgccaacga cttgcatggc ggcagtcgag gtatccactt caatgccacg cggctgcatc
      361 atggcttcga gcagtttcca ggaatcgttc ggcaatagtt ccagcagacc ttcgtccacg
      421 gccacgagcg ctacttcacc ggctctgggt ggtttgccgc cagctcgctg cgttacttgc
      481 actgacacgc gtgccttttc gcgcacctta taagtttcct tgtccgcgct gaccttgact
      541 gtgagttcat gcgcccgcca gccgacgttg atttccgcca cgcccatctt gaaggccggc
      601 ttgcccagat ccaccagcgc agtaggctga acatcggaca cacggccgcg gacgacgagc
      661 gcggatacaa atacgttcgg cgcgtagttg cccttcagcg gcactttgat taccggcgcc
      721 tcgccagaca gtggctgcac gaaggaatcg atcacgcctt cacgttccac cgtgatcagc
      781 gcggtagcct ggcgaaacgg catgcgcacc tggaactgcg ccgtctgtcc cggttcgtag
      841 tgtttgcgtt cggggatcag gtcgatgcga tcatcgttgc tttgatcgaa ccaccacgcc
      901 tgcgtgcccg ccacccaggc cgaggcattc gcgtaggcaa gatggccctc gcggtccttc
      961 gcaaccgcgc ccatgacgat attgccggac accggcggct tgaccgcgca gaacaaacgg
     1021 cccttgtcat cggtcttgcc ctggcaggtg gagtcgactt tattcacctc cgcaccgctc
     1081 tcgtagccgt agaagccgcc aatcaaccgc ttgcggtgtg aaaacagcgt acgcgcgtac
     1141 agttgcacat cgacttgctg gttcttgatc ggcttgcccg tcagatcgag tgccagcacc
     1201 tggaatttca gttcatcttt ggaaagcgtc cacgcatcgg gtttgatgcc gagcagtaat
     1261 ttagccggcc aaaggggcac gcgggtagag gccgtcagta cttcgccgtt cggatcggcg
     1321 tactcaagtt ccgcgttgac ttcctgcggc gtgtccgcga ccggcagacc cggcaacctg
     1381 acgcgcgccg cgcccgcgct atccagtgcg agttcgacgg tcttaagcgg gcgcacgcca
     1441 ttgaccggct gtgccggaat cggttcgtcc gtgtccgcat cgcccaactc gtattcgccg
     1501 ctgaaccact gcgcgcccga aggttgctcc ggaccttcca ccaccttgcc gttggcaaat
     1561 acaaaaccgt cgtaatcggc aaacgcgaca tttttcggcc gcaccactgc acgtaccttg
     1621 acgggcgcgt aggccgccat cccgcctgcc aggtagcgca gatgcacgtc gacatcgatg
     1681 ctctgcgcat tcaccggcat tgccttgggt ccgaagattt ccgctttcat cagcggcaca
     1741 cgaaaatctt cgacgcggaa acttcccgcc gagcgcgtcg tgccgcgtgc gcctggacgc
     1801 acgtcgagcg tgtcggtcat ttgtaccagg tatgtgccct ttttcgcgcc cgctggtatc
     1861 tgccagctgc tttcggcaat gttgcgcgtg tcccatttca ccgggatttc gtatttctgc
     1921 gcggaaccct gatgcatgat cgtgatgctt tttggcaggt tgtccgcggg aacaaacgaa
     1981 aagcctttgg cctcgtgcct gcgatagtag tgtttcatgt tgacggtttc gccggcgcgc
     2041 aacaacgtgc ggtcgaacac cgtggtcacg agataaggcc cgttccagcc gccctggcgc
     2101 aagttaaagc gccagcgcgc aatgccctcg ttccactccg acatgacaaa agacaaatca
     2161 tccgcgagcc gcgcggagac gatcagctgc ttgtcatatt cgtgtacgca ggcgggcaga
     2221 ctctcccgga ccggcaacgc ggccgtgaca cgcgcgcgtc ccagcacatc ggtctggccg
     2281 cgaaagaaca cgcggccgga acaatcctgt atcgccactt gcgccttcgg caccggcacg
     2341 ccgctatcca gcgccgtcac ccacaccagc gcattttcac ggccccattt gaaatgcact
     2401 gcaagattcg tgaccagtgc cgcggattgg acgtaatacg gagcgcgggt gttcagcaac
     2461 gcggcgccca atcgcgggct ggcaagttca accacataga agcctggctt cttcagcggt
     2521 atgccgacga cttcatagga gcgcgcgccg tcgggccgcg gcacctggag gcgcgtgacc
     2581 cggtcgcctt cggcaaaaat ggagttctga cccagccggt actgcaggac atccttgttg
     2641 gtcttctggt cgtagcgcca gcgttcgttc tccatttttc gcaagcgctt catccattcc
     2701 agcatggcca tcacgtctcc gtcagccacg cgcgaataac tgccgggcac gccagcgtcg
     2761 ctttgcttgg cttcgtcgag cttgttcttc agccagttga tcgccgcatc ggctttttcg
     2821 gtaacgttgc ccgcggcatc gccttgcctc ccggtatgcg ccatgcgcgc ttcgagcacc
     2881 gcatccagat tacgcagtgt caccggcatc atgccatcgc cctttgcttc gatgatgccg
     2941 aagcgcgcgg caaacttcgc aagcggcggg tattcatcgg tgcgcaccaa taagggaaag
     3001 cgtttctggt tggccagttt gcgtccggcg tcatcggtca catcgggggg caagtccagc
     3061 gcaaactggc tgcgttcggg aaatggccca ctgaaaaata cttcgtcgac gaactcaggc
     3121 gccttgttcg catcgggcaa tcccgcagcg atggtcttgc cttgcacctt gagcacgatc
     3181 atctgtgccg tagcgcgggg tatcggtgcg gaaaatgcga gccgcatggg caggatcggc
     3241 aaacactggg catccttgtt cactcgatcg caggtgaacc gcgcctggaa tgtgtcacgt
     3301 accttatatg cgagcgactg gtcctggccg gtgggaatcc cgctatcgga catgatgcct
     3361 ttgccccaca ccaggctgac ttgcccgttg ttgggcaggc ggcgctggca ttgaagtgcg
     3421 acgagcggaa gtttggcgcc ggcgtcaccg ttcaaaaact gatccaggcg cgaccccctg
     3481 ccgaacggcc tgccgtccat gataatttcg cggccgtcct tgaacagtaa gcgcaaccag
     3541 cgatcgacaa aatcacggcg ggagtccagc agcgccttgc gttcttcgcc gccgatcacg
     3601 cgcactggta tgcgctcggt tacgccacgt acgtcgcaat acgcattttc cagaacggat
     3661 tgatccgtcg ccggagcatc gagccccagc aggaaaatct gctcttcatc gatattgttt
     3721 ccctcgaacg gcaacatctc cagtattgcc ggtccgccag tattgaattc aaacgtctga
     3781 tcaccagcga gcgtgtctcc ggcaagcgac acaacatcgg atctcagttt gaaagtgcag
     3841 cgcataccgg ccggcagatc atgtgcgaag tcaaaaaccc aatttttctg gtcg
//
LOCUS       DATMZQ010000404         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4119921, whole genome shotgun sequence.
ACCESSION   DATMZQ010000404 DATMZQ010000000
VERSION     DATMZQ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4119921"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..608
                     /locus_tag="VN496_12920"
     CDS             228..608
                     /locus_tag="VN496_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXF17903.1"
                     /translation="MQKQSGFTLIELVVVIVILGILAAVALPRFIDLSTQAGAAGAQG
                     VAGALSSATSINYAAKKAGNASGVTVSSCLTALNTLTPTSMPTGYTLGAGTACTALDG
                     GTATCPVTNTASGQVASATIICVP"
     gene            678..1187
                     /locus_tag="VN496_12925"
     CDS             678..1187
                     /locus_tag="VN496_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXF17904.1"
                     /translation="MVPPAPSRFIAITRSRAGFTLIELVVVIIVVGILAVVVIPRINT
                     KTFDTAGFYQEALASVRYAQKEAVAKRRVTCVGLTATSITVRFAKNTSSFTCDTELTS
                     PRGVNPFTVTARAGVTLASAPAIATLYFDPLGRPFDALNSASPQRTLTISGDGTQSFV
                     IEPETGYVH"
     gene            1177..1770
                     /locus_tag="VN496_12930"
     CDS             1177..1770
                     /locus_tag="VN496_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXF17905.1"
                     /translation="MSIEHCAVNRLVALRRAQAGISLVELIIFVIIVSIGVAGILTVF
                     NLTVQKSADPQIRRQMLAVAEALLEEVQFKSFTICDPDDPNASLPTTATSADCTGGTG
                     GANDESKLPLGPEGGETRGIFDNVSDYNGLTLAPFSDINGTVIAGYSATVSVAQQQLE
                     ASIPLAASLRITVTVTHGSDSLSLSGYRLRYAPRSLP"
     gene            1767..2576
                     /locus_tag="VN496_12935"
     CDS             1767..2576
                     /locus_tag="VN496_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXF17906.1"
                     /translation="MTGRWKWQTMSGPNRGAIRGFTLTEAIMVIVITGILASMVAVFI
                     QKPVQGFFDTTRRAALVDAADTILRRLSRDVREALPNSVRVSAGTALEFLHVRSAGRY
                     REQGPGDVLDFGTNDTSFAVLGPTVNVQSGDSIVVYNLGVAGANAYQGTNISLAVAPF
                     GASLINVAIAGFAFPFSSSAKRFQVVDTPVSYICSGTQMRRYSGYAIVSAQPVPPGGT
                     GSLIASQLSGCSFAYATGASEREALVTVSLALTDTGETVSLIYQIHVNNAP"
     gene            2596..3024
                     /locus_tag="VN496_12940"
     CDS             2596..3024
                     /locus_tag="VN496_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshP"
                     /protein_id="HXF17907.1"
                     /translation="MPSAIFLIVLLAILGAMVVTVSGLQHTASARDLMGARTLQAARA
                     GIDWGAYQVLQQAAALGAGVCPASTAIAMPAGTDLTGLTVNVTCSLSNADEGGRTAAG
                     GNRLEFYVITATACNQPPCPNTTNPGSAYVERQLQASIGR"
     gene            3025..>4082
                     /locus_tag="VN496_12945"
     CDS             3025..>4082
                     /locus_tag="VN496_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17908.1"
                     /translation="MEDLDAVNKATMQHEHGKIWLAARRVRITTVASVLLITACLLAS
                     NAQAAARFARSSGNWSTLTWSATSCAAGTGAALPTAADDVTICNGFTVTVNVSPTVQS
                     ISIPSGATLTTLQITSAQTLTVTGNLTAVAGTGSGDHRQILLSNNAVLNVNGNVTLNG
                     SGNDNRFALLQISGATATIGGNLVIDGLGGSDRARVTLANTSTLTVNGDITLNTGGLL
                     DNGNTTSSVIFLRGDFDHANADNDYVSTGGIFQVDGAGPQTFGGGAGITTTFYRLVIN
                     KASGDLTLGHNIIVAGAAASSLLVLTQGRIVTAANSVGVGAAGATSTTISGASTASYI
                     VGNLRRYIPTGAQPGVMFP"
BASE COUNT          845 a         1184 c         1190 g          863 t
ORIGIN      
        1 aacgatcaag tgactctttc ggcaggtggt gcggcggcag gctctaccat cacgattgac
       61 attaacggcg ccacgaccgc ggcgaactgc agagtttcat atacctccgc gctcgctaat
      121 gcgtcgccaa ctattgcctt accaatagcg ccggattgct cttgagaatg cagctgcaat
      181 cagtattagt cttaaacgag ttcttatagt ctagacagga gatgggaatg caaaagcaaa
      241 gcggttttac gttgattgaa ctggtagtgg tgatcgtcat cctcggcatc ttggctgcgg
      301 tagcgttgcc aagatttatc gatttgtcta cccaagccgg tgcggctggc gcccaaggcg
      361 tggcaggcgc gctatcgtcg gcaaccagca ttaactacgc tgcgaagaaa gccgggaacg
      421 cttcaggcgt gacggttagt agctgcttga cggcactaaa tacgttgaca ccgacgtcaa
      481 tgccgaccgg ctatacgctc ggcgctggaa ctgcttgcac agctcttgat ggtggcacgg
      541 ctacttgccc ggtgaccaac acagcgtcgg ggcaagttgc cagcgcgaca attatttgcg
      601 tgccctgagt tcaaattaac aagacgcttt gcgaaagcgc gaggcgaatc gcctcgcgct
      661 tctcttgtga tcctccaatg gtgccaccag cgcctagcag gttcatcgca ataacccgca
      721 gccgtgcggg cttcaccctg atcgaacttg tcgtggtgat catcgtcgtc ggcatcctgg
      781 ctgtcgtcgt gataccccgc atcaacacca aaacttttga caccgctggt ttttaccagg
      841 aggcgctagc ctcggtacgc tacgcgcaaa aggaagcagt tgcaaaacgc cgggtcacct
      901 gcgttggcct tacggcaact tccatcacag tccgttttgc aaagaacaca agttctttca
      961 cttgcgatac cgaactgacc agcccccgcg gggtaaatcc gtttaccgtc acagcacgtg
     1021 cgggcgtcac actcgcaagc gcgcccgcga tcgcgacgct ctatttcgat ccgctcggac
     1081 ggccttttga cgcgcttaat tcggccagtc cgcaacggac tttgactatt tctggcgacg
     1141 gtacgcaaag cttcgttatc gagccggaaa ccgggtatgt ccattgaaca ttgcgcggtg
     1201 aacagactgg tcgccctgcg ccgcgcgcaa gccggaatat cgttggtcga actcatcatt
     1261 ttcgtcatca tcgtcagtat tggcgtggca ggcatcctga cggtgttcaa tttgacggtg
     1321 caaaagagcg ccgatccgca gattcgacgg caaatgctcg cggtggccga agccctgctg
     1381 gaagaggttc aattcaaatc tttcacgatc tgtgatccgg atgacccgaa cgcttcgctg
     1441 ccgactacgg cgacttccgc cgactgtacc ggcggcaccg gaggcgccaa tgacgaaagc
     1501 aagcttccgc tcgggccgga aggcggggaa acccggggca tctttgacaa cgtcagcgat
     1561 tacaacgggc tcactctcgc acctttctcc gacatcaatg gcacggtgat cgctggctac
     1621 tcggctacgg tcagcgttgc ccagcagcag cttgaggcca gtattccgct ggccgcgtcg
     1681 ctgcgcataa cagtcacagt cacgcatggt agcgattcgc tttcgttgag cggatatcga
     1741 ctgcgctatg cgccaagatc gctgccatga ccggacgctg gaaatggcaa acgatgtcgg
     1801 gccccaaccg gggcgcgatc cggggtttta cattgaccga ggccatcatg gtcattgtca
     1861 ttaccgggat tctggccagc atggttgccg tcttcatcca gaaaccagtg caaggtttct
     1921 tcgatacgac gcggcgtgcc gcgctggtgg acgcggcaga taccattttg cggcgactaa
     1981 gccgtgacgt gcgagaggca ttgccaaata gcgtgcgggt cagcgctggc acagcgctcg
     2041 agtttctgca tgtccgatcc gccgggcgtt atcgggaaca aggtcccgga gatgttctgg
     2101 acttcggtac gaacgacacc tcatttgcgg tgttggggcc aactgtcaat gtgcagtcgg
     2161 gcgattccat cgtggtatac aaccttggtg ttgccggcgc gaatgcgtac caagggacaa
     2221 atataagtct cgcggtcgcg cctttcggcg catcgttgat caatgttgca attgccggct
     2281 tcgcattccc tttttcctca tcggcaaagc gcttccaggt ggtcgatacg ccggtgagct
     2341 acatctgttc cggaacccag atgcggcgct attccggcta tgcgattgtg tctgcacagc
     2401 cagtgccgcc cgggggcacc ggctccctga ttgcatccca gctctctggg tgcagcttcg
     2461 cctatgcgac cggtgcgagc gaacgagaag cgctggttac agtgagcctg gcgctcacgg
     2521 acaccggcga aaccgtcagc cttatttatc agatacacgt gaacaatgcc ccgtaacttc
     2581 tcagcgggat ttgccatgcc cagcgcaatt tttctgattg tattgctggc gatcttgggt
     2641 gcaatggtcg tcaccgtgtc tgggctccag cacactgcct cagcgcgcga cttgatgggg
     2701 gccagaacgc tgcaggctgc acgggcgggc atcgactggg gcgcctatca ggtactgcag
     2761 caggccgccg ccttgggtgc tggagtctgt cccgcttcga ccgccatcgc catgccggca
     2821 ggtacggacc tgaccggact aacggtgaat gtgacctgct cgcttagtaa tgccgatgaa
     2881 ggtggccgaa ccgccgccgg cggaaatcgg ttggagttct atgtcattac cgctactgca
     2941 tgcaaccagc cgccgtgccc gaatacgacc aatccagggt cggcctatgt ggaacgacag
     3001 ttgcaggcgt ctatcggacg atgagtggag gaccttgacg cggtgaacaa ggcgacgatg
     3061 caacacgaac acggcaagat ttggttggct gcacgacgcg tgcgtataac cacggttgcg
     3121 tccgtgttgc tgatcacagc gtgcttgctg gcatcgaacg cgcaggcggc cgcgcgcttt
     3181 gcacgcagct ccggtaactg gagcaccctc acctggtcgg ctaccagctg tgcggcaggg
     3241 acgggagcgg ctttgccgac cgcagccgac gacgtgacga tttgcaatgg cttcacggtc
     3301 actgtgaacg tatctccgac ggttcaatcc atcagcattc ccagcggtgc cacccttacg
     3361 accttacaga tcacgtcggc gcaaacgttg acagtgacag gaaatctaac ggctgtagca
     3421 ggtaccggca gcggcgacca cagacaaatc ctgctgtcca ataacgcggt ccttaacgta
     3481 aatggcaacg taaccctgaa tggcagcggc aacgataacc gctttgcgtt gctgcagata
     3541 agcggcgcaa ccgccacgat cgggggaaac ctggtgatag acggccttgg tggaagcgat
     3601 cgggcacggg tgactctggc caatacatcg acccttacgg tgaacggcga tatcacgctg
     3661 aacaccggtg ggttactcga caacggcaat accacaagca gcgtgatttt cctgcggggg
     3721 gatttcgatc acgccaacgc agacaacgac tatgtcagta cgggcggcat tttccaggtg
     3781 gacggcgctg gcccgcaaac tttcggtggc ggtgccggca ttacgacgac tttctacaga
     3841 ttggtcatta acaaggcatc gggtgacctg actctcggcc ataacatcat cgttgcaggc
     3901 gccgccgcca gttccttgct ggtgctgacg caggggcgca tcgttacagc ggcgaactct
     3961 gttggcgtcg gggccgcggg cgcaacgtcc acgactatca gcggggccag tacggcaagc
     4021 tatatcgtgg gcaatttgcg caggtatatt ccaaccggtg cgcaacccgg cgtcatgttc
     4081 cc
//
LOCUS       DATMZQ010000405         6779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2050459, whole genome shotgun sequence.
ACCESSION   DATMZQ010000405 DATMZQ010000000
VERSION     DATMZQ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050459"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VN496_12950"
     CDS             <1..900
                     /locus_tag="VN496_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HXF17909.1"
                     /translation="WFTFSYIPVAHMVWFWPGPDAYTAANVVDAQNAKAGLLWQWGAL
                     DFAGGTVVHINAGIAGIVGAYVMGKRIGFGKEAMSPHNLTMTMIGASLLWFGWFGFNA
                     GSALEANNSAALAFMNTYLATACAVLSWMFAEWLFKGKPSMLGAASGAVAGLVAITPA
                     AGNVGIPGAFVIGLAAGVVCLWGVNGLKKLLGADDTLDVFGVHAVGGILGALLTGVFN
                     APSLGGPGFVNNWFEMKPGFESIAAQFWIQAKAVGVTVVWSAVVAFIAYKIADLVTGL
                     RVPEEQEREGLDITSHGESAYHM"
     gene            1206..2156
                     /gene="gshB"
                     /locus_tag="VN496_12955"
     CDS             1206..2156
                     /gene="gshB"
                     /locus_tag="VN496_12955"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HXF17910.1"
                     /translation="MKIAFVLDPLETIKTYKDSSYAMMREAASRGHSLAVLHQENLVL
                     LDGQTVAYVRDLVLLPDEAQWFRMSEPRVVRLDTFDAVMMRKDPPFDMEYIYSTYLLE
                     LAQRQGACVVNDPRAVRDHNEKLAIAEFPQFTVPYLVTREESLIREFLAKYGDIIVKP
                     LDGMGGTSVFRLHQKDHNIGVVIETVTHYGRRTVMAQRYIPEIVKGDKRVLIVDGEPV
                     PYCLARIPKQGETRGNLAAGGTGVAQPLSPRDLEIARALGPRLKAAGLLLVGLDVIGD
                     YLTEVNVTSPTCMREIADQTGCDPAAMMIDALEKLSSARQ"
     gene            2251..2667
                     /locus_tag="VN496_12960"
     CDS             2251..2667
                     /locus_tag="VN496_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HXF17911.1"
                     /translation="MVGILIITHGALGESLIHSATHVMGARPMQLAHIGVGMADDPQQ
                     VLPRAIALLQNLDQGGGVLVLSDVYGATPSNIAGRLLTPGKIEGVAGVNLPMLVRALT
                     YRNEPLKTVVAKAVSGGIEGVMQIPIVQVRDAKTGH"
     gene            2648..2917
                     /locus_tag="VN496_12965"
     CDS             2648..2917
                     /locus_tag="VN496_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXF17912.1"
                     /translation="MQKQDIEIVNKLGLHARASAKLTQLASRFASEVWISRDGRKVNA
                     KSIMGVMMLAAAQGSLVSIETEGPDETQAMAALVGLINEKFGEGE"
     gene            2988..4712
                     /gene="ptsP"
                     /locus_tag="VN496_12970"
     CDS             2988..4712
                     /gene="ptsP"
                     /locus_tag="VN496_12970"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXF17913.1"
                     /translation="MSFTIHGIGVSGGIAIGHAQLVSHAQLEVAHYDISRSRVADEKA
                     RFDAAVVTARAELASLGEHIPAGAPAEFEAFLNVHLMILDDPTLSETPKQLIETLQCN
                     AEWALKLQMDALIEQFESIEDGYLRDRKTDVIQVVERILKVLMGRPGEVLARNAPEEN
                     SILVAHDLSPADVIQFRSHRFASFITDLGGATSHTAIIARSLNIPSVVALHHARTLIR
                     ENEVLLVDGSNGVVIVNPDKTVLAEYKLRQEQWELERLKLKRLKRTKTTTLDGTAVEL
                     QANIELPDDVADAKENGADGVGLFRTEFLYLNRGDQPSEDEQFEAYRRVVQEMEGRQV
                     TIRTFDLGADKHVDAAERVAPNPALGLRAIRLCLAEPQMFHRQLRAILKASHYGKVRI
                     LIPMLSSANELNQTMHHIEEAKLSLDREGIAFDKRIPVGGMIEIPAAALSLSTFTRTL
                     DFLSIGTNDLIQYMLAIDRADDTVAHLYDPLHPAVLNLVAHIIRSANRADVPVTVCGE
                     MAGDVRLTRVLLGFGLRQFSMHPAHLLSVKQQVLKSNLQEVIVFTQKMLRTDDSDKVE
                     QLLARMNR"
     gene            4781..5842
                     /gene="holA"
                     /locus_tag="VN496_12975"
     CDS             4781..5842
                     /gene="holA"
                     /locus_tag="VN496_12975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXF17914.1"
                     /translation="MRIDSEQLSQHLSRGLKNLYVIFGEELLLALEAADRIRAKALEQ
                     DFTERRVLIADTGFDWSELAMASSSLSLFAPRTILDLRIPNGKPGKDGSDGLQQLATS
                     LSADTLILITLPALDRQAQASKWFTALEKAGVAVHAAAVKRERLGQWLAGRLAQQDQQ
                     ADEAIIQFLVDCVEGNLMAAHQEVQKLSLLFPKGALPGEEVRNAVVDVARFNVFDIGA
                     ALLKGDRVHYVRMLDGLRAEGTAAPLVLWAISEEARTMAKVKAAMSSGQPLAQALRDT
                     RVWGARQDLMPGALRRLEQSQLVKALRDAADIDRMIKGLRDGDVWDALLQLGLDLMAP
                     MRQGRAAKNRGKIGADIPE"
     gene            5848..>6779
                     /locus_tag="VN496_12980"
     CDS             5848..>6779
                     /locus_tag="VN496_12980"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXF17915.1"
                     /translation="MDIQSYMHSIGRAARNASRAMAKADSGAKNHALMTIAQAAERDV
                     ARLLDANRKDIEAARAKGLDSALLDRLALTPKSVSDMAEGLRQIAQLPDPVGEISDMR
                     YRPSGIQVGRMRVPLGVIGIIYEARPNVTADAAGLCLKSGNAAILRGGSEAIHSNHAI
                     GACVHEGLGAAGLPESAVQVIETTDRAAVGELITMKDYVDIIVPRGGKGLIERISSES
                     RIPVLKHLDGVCHVYVDDRADLDKALRIADNAKTQRYGTCNTMETLLVHQAIAARFLA
                     PLAKIYLEKGVELRGDDAARKIVADMTPAVDEDW"
BASE COUNT         1480 a         1898 c         2048 g         1353 t
ORIGIN      
        1 tggttcacct tctcttacat tccggtcgcg cacatggttt ggttctggcc cggcccggac
       61 gcgtataccg cggccaatgt ggtcgatgcg cagaacgcaa aggccggact gctatggcag
      121 tggggtgctc ttgattttgc cggcggtacg gtagtgcaca tcaacgccgg tattgccggc
      181 atcgttggcg catacgtgat gggcaagcgg ataggcttcg gcaaggaagc aatgtctccg
      241 cataatctca cgatgacaat gatcggcgca tcgctgttgt ggttcggctg gtttggattc
      301 aatgccggct cggcactgga agccaacaat tccgcagcac tcgccttcat gaacacctac
      361 ctggcaaccg cttgtgccgt gctgtcatgg atgtttgccg agtggttatt caaaggcaag
      421 ccttccatgc tcggcgccgc atcgggcgca gtggccggac tggtcgccat taccccggct
      481 gcaggcaacg tcggtatccc cggcgcattc gtgatcggcc tcgctgccgg tgtcgtgtgc
      541 ctgtggggcg taaacggctt gaagaagttg ctgggcgcgg acgacacgct ggacgttttt
      601 ggggtgcacg ccgtgggcgg tattctcgga gcgctgctaa ccggtgtatt caatgcgccg
      661 tcgctgggtg ggccgggctt cgtgaacaac tggttcgaaa tgaagcccgg ttttgaaagc
      721 atagcggccc agttctggat tcaggctaag gcggtgggag taaccgtcgt ctggtccgca
      781 gtagtagcct tcatcgcgta caaaatagcc gatctggtta cgggcttacg cgtgccggaa
      841 gaacaggaac gtgaaggcct tgatatcacc tcccacggcg agtcggcgta ccacatgtaa
      901 atctcctccg gtaagttcgc ttacctttca cgggcgcctt cgggcgcccg ttttttatga
      961 aggaatgcag ctatgccaac gatggtgtca aaatattcct gcatccacgc tcgcttcacc
     1021 gtatgtgcag ccgcaatccg cgttatgaac agcccctctg ctacaatctg ccgctgccat
     1081 ttttggacgt gcccgagcat gccgccgagg cgggaaggac agtaattgat ccgcgcggca
     1141 gaggcaccta acgcactagc agtctgccgc tgcgctttct gtgcagtagc acttgcagat
     1201 accacatgaa aatcgccttt gtccttgatc cgctcgagac catcaaaacc tataaggact
     1261 cgtcctacgc gatgatgcgt gaggctgcgt cgcgtggcca ctcactggcg gtattgcatc
     1321 aggaaaatct ggtgttgctc gatggacaaa cggttgccta tgtacgggac cttgtgctgc
     1381 tgccggatga agcacagtgg ttccgaatga gcgagccgcg cgtggtgagg ctggatacat
     1441 tcgacgcggt gatgatgcgc aaggatcccc cattcgacat ggaatatatc tacagcacgt
     1501 atctgcttga attggcccag cggcaaggcg cctgtgtagt gaatgatccg cgcgccgtgc
     1561 gcgatcacaa tgaaaagctg gcaatcgctg aatttccgca gttcacggtt ccctacctgg
     1621 tcacacgaga agaaagtcta atccgggaat ttcttgcaaa atacggcgac atcatcgtca
     1681 agccgctgga tggaatggga ggaacgtcgg tatttcgcct gcatcagaaa gatcacaaca
     1741 tcggcgtcgt gatcgaaacc gtgacgcatt atggaaggcg aacggtcatg gcccaacggt
     1801 acatcccgga gatcgtcaaa ggcgacaagc gtgtgctgat tgtcgatggt gaaccggtgc
     1861 cttactgcct cgcccgcatc ccgaagcaag gcgagacgcg cggaaacctg gctgctggag
     1921 gcaccggtgt tgcccagccg ctttccccgc gcgacctgga gattgcgcga gcgcttggtc
     1981 ctcgcctgaa agcagcaggc ctgttgctgg taggcctgga tgtgatcggc gattatctga
     2041 cggaggtcaa tgtgaccagt ccgacctgca tgcgcgaaat tgccgatcag accggctgcg
     2101 atccggcagc aatgatgatt gatgcgttgg aaaaattgag cagcgcccgg cagtgagtcg
     2161 cacggcatct gccgctggtt tcaatcgtgc tgaaagctcc ggcaaaggta gaattcagga
     2221 cttgcccggt gtaaacatgc tcaagcgtac atggttggaa tactgattat cacccacggc
     2281 gcgctgggcg aaagcctgat ccacagcgca acccacgtca tgggcgccag gccgatgcaa
     2341 ctggcacata ttggcgtagg catggcagat gatcctcaac aggtgctgcc gcgagccatt
     2401 gccctgttgc agaacctgga tcaaggcggt ggcgtccttg tgctttccga tgtgtacggc
     2461 gcaacaccca gtaatatcgc gggcagatta ctcacacccg ggaaaataga aggtgtcgcg
     2521 ggagtgaacc tgccgatgct tgtgcgcgca ctgacttatc gcaatgaacc gctgaaaaca
     2581 gtcgtggcaa aggccgtctc gggcggcatt gaaggggtca tgcagattcc tattgtccag
     2641 gtgcgcgatg caaaaacagg acattgaaat cgtgaacaag cttgggctgc acgcccgggc
     2701 gtccgccaag ctgacccagt tggcgagccg gtttgcatcg gaagtctgga tttcgcgcga
     2761 cggccgcaaa gtcaatgcca aaagcatcat gggcgtgatg atgttagcag cagcacaggg
     2821 ctcgctggtg tccatagaaa cggaaggtcc cgacgaaacg caagccatgg cggcgttggt
     2881 cggattgatc aacgaaaagt tcggtgaagg ggaatagcac ggagacgaag gacgaggagc
     2941 gtggttcgag cgggcatgcc attcccgccc agccctcggc tgctttgatg agtttcacca
     3001 tacacggcat cggcgtatcg gggggcatcg ccattggcca tgcgcagctc gtgtcccatg
     3061 cgcagctcga ggtcgctcac tacgatattt caaggtcacg cgtcgcggac gaaaaggcac
     3121 gctttgacgc ggccgtggtc accgcacggg ccgaacttgc tagcctcggt gaacatattc
     3181 cggccggggc gccggccgaa tttgaggcgt tcctgaacgt gcacctcatg atccttgacg
     3241 acccgactct ctcggaaacg cccaagcaac tgatcgaaac gcttcagtgc aacgcggaat
     3301 gggccctcaa gctgcagatg gatgctttga tcgaacaatt tgaaagcatt gaagacggct
     3361 atttgcgcga tcgcaaaacc gatgtcatcc aggtagtgga gcggatcctg aaggtcctta
     3421 tggggcggcc cggcgaagtc ctcgcgcgca acgctccgga agaaaacagc atcctggtcg
     3481 cgcacgatct gtcgcctgcc gatgtcattc aattccgcag ccaccgcttc gcgagtttca
     3541 tcacggatct tggcggcgca acttcgcata cggcgattat cgcgcgcagc ctcaacattc
     3601 catcggtagt cgcgttgcat catgcacgca cactgattcg tgaaaatgaa gtcctgctgg
     3661 tcgatggcag caacggcgtc gttatcgtca atccggacaa aacggtgctg gccgagtaca
     3721 aactgcgcca ggaacaatgg gaactcgagc gtttaaagct caaacgcctg aaacggacga
     3781 agaccaccac cctggatggc accgctgtcg aactgcaggc gaacatagaa ttaccggatg
     3841 atgtggccga cgcaaaggag aacggggccg acggtgtggg actgttccgt actgaattcc
     3901 tgtacctgaa tcgcggcgac cagcccagcg aggatgaaca attcgaagct taccgacgcg
     3961 tcgtgcagga gatggaagga cgccaggtca cgatccgcac ttttgacctg ggagctgata
     4021 aacatgtcga tgccgccgaa cgtgtagcgc ccaaccctgc actcggatta cgtgccatcc
     4081 ggctatgcct ggccgagccg cagatgtttc accggcaatt gcgcgccatc ctgaaggctt
     4141 cccattacgg caaggttcgc atcctgatcc cgatgctgtc ctccgcaaat gagctgaacc
     4201 agacgatgca tcacatcgag gaagcgaaac tcagtctcga tcgggaaggc attgcttttg
     4261 acaagaggat tccggttggc ggcatgatag aaattccggc tgcggccctg tcgctctcta
     4321 cttttacccg cacgctcgat tttctgtcca ttggcaccaa tgacctgatc cagtacatgc
     4381 tggccataga tcgcgcggac gatacggtgg cccatctgta tgatccattg catccagcgg
     4441 tgctgaacct ggttgcgcat atcattcgca gcgccaaccg cgccgacgtg cccgtaacgg
     4501 tgtgcggcga aatggccggc gacgtgcgac taacgcgggt gcttttgggt ttcggcttgc
     4561 ggcaattctc aatgcaccct gcgcacctgt tatcagtgaa gcagcaagta ctcaaatcga
     4621 atctgcagga agttatcgtc tttacgcaga agatgctcag gaccgatgac tctgacaagg
     4681 tcgagcaact tctggcgcgg atgaatcgtt gatgcatcgg catttacgac tttggcggca
     4741 ctgctgccaa gcttaacttc gccgccaatc ggcagcagca atgcgcattg actccgaaca
     4801 gctttcccag catctgtccc gtggactgaa aaatctttac gtcattttcg gcgaggagct
     4861 gctgctggcg ctggaagcgg ccgatcgcat tcgtgcaaaa gccctggagc aagatttcac
     4921 ggaacgccgc gtcctgatcg cagacaccgg cttcgactgg agcgaactgg ccatggccag
     4981 cagcagtctt tccctttttg cgcctagaac gatcctcgat ttgcgcatcc ccaacgggaa
     5041 gcccggcaag gatggcagtg acggcttgca acaactggcc acctcgctca gtgctgatac
     5101 gctgatcctg atcacgctgc ccgccctgga tcgacaggcg caggcatcga aatggtttac
     5161 agcgctggaa aaggcaggtg tggcggtgca cgcggctgcg gtcaaacgcg agcgtctagg
     5221 ccaatggctc gcagggcgcc tggcgcaaca agatcagcaa gctgacgaag ccatcattca
     5281 attcctggtc gattgtgtcg aaggcaattt gatggccgcg catcaggagg tacaaaagct
     5341 gtccttgctg tttccgaaag gtgctctgcc aggagaagaa gtaaggaatg ctgtcgtcga
     5401 cgtggcgcgg ttcaacgtat tcgacatcgg tgcagcattg ctgaagggcg atcgcgtgca
     5461 ctatgtgcga atgctggatg gcctgcgtgc cgaggggact gccgcaccgc tggttttatg
     5521 ggcaatctca gaagaggcgc gcacgatggc gaaggtcaag gcggccatgt catccggcca
     5581 gccgcttgca caagccttac gcgataccag agtgtggggc gcccgccagg acctgatgcc
     5641 aggcgccttg cgtcgcctgg agcaatcgca attggtcaag gcattgcgtg acgcggcgga
     5701 catagatcga atgatcaagg gactgcgcga cggcgacgtg tgggacgcgc tactgcagct
     5761 cggactggac ctgatggcac cgatgcggca aggccgcgca gcgaagaata ggggtaaaat
     5821 cggcgccgat attccggaat gaatcacatg gacatccagt cctacatgca ttcgatcggc
     5881 agggcggcac gcaacgcatc gcgggccatg gcaaaggccg actcgggtgc gaagaatcac
     5941 gcgctaatga cgatcgcgca ggccgccgag cgcgatgtgg cacgtttgct ggatgccaac
     6001 cgcaaggata ttgaggcagc ccgagccaaa ggcctcgatt ccgcgctgct cgaccggctg
     6061 gcgctgactc cgaaaagtgt gtccgacatg gcagaaggac tgcgccagat cgcgcagttg
     6121 cccgaccccg taggtgaaat ttcggacatg agataccggc cgagcggcat acaagttgga
     6181 cgcatgcgcg tgccgctggg cgtgatcggc atcatctacg aagcgcggcc gaatgtaacc
     6241 gcggacgccg cagggctgtg cctgaagtcg ggcaacgcgg ctatcctgcg cggcggatcc
     6301 gaggccattc attcgaacca tgcgatcggc gcttgcgtgc acgaaggcct gggcgcagca
     6361 ggattgcccg aaagcgccgt gcaggtgatt gaaacaaccg atcgtgcagc cgtcggcgaa
     6421 ctcattacca tgaaagatta cgtcgacatc atcgtaccgc gcggcggcaa ggggctcatc
     6481 gagcgcatct ccagcgagtc gcgcatcccg gtgctcaaac atctggacgg agtgtgccat
     6541 gtctatgtcg acgaccgtgc cgatctggac aaggcgctcc gcatcgccga caacgccaag
     6601 acccagcgct atgggacctg caacaccatg gaaacactcc tggtacacca ggcgattgca
     6661 gcgcgctttc tggcgccgct ggccaaaata tacctggaga agggcgtcga actgcgcggg
     6721 gacgacgcag cgaggaaaat tgtggctgat atgacgccgg ctgtcgatga agactggta
//
LOCUS       DATMZQ010000406         4330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1545384, whole genome shotgun sequence.
ACCESSION   DATMZQ010000406 DATMZQ010000000
VERSION     DATMZQ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545384"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1106
                     /gene="denD"
                     /locus_tag="VN496_12985"
     CDS             129..1106
                     /gene="denD"
                     /locus_tag="VN496_12985"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HXF17916.1"
                     /translation="MKVFITGGGGFLGYRLALRLLDRKTLTGPDGKSEVISEIKVFDA
                     AFAANLDRRLQYIQADITDADAVAQALDRDTACIFHLAAVVSAHAEAEFDVGYRVNLD
                     GTRNLLEACRDLDRPPRLVFASSLAVFGGTLPAIVDDRCTPMPQTSYGVQKVIGEYLV
                     NDYSRKGFIDGRSLRLPTIVVRPGKPNLASTSFASGMFREPLNGVVCEVPVQDNIEMW
                     ILSPGKVLDAFIHANELPESAWGTNRAVTLPGITFSLKQGVAALRRIAGDEVANRITF
                     KPVDRINNMVKTFPARFDPRRALDMGFTADSDIDAIIKDYIQGERIKVA"
     gene            1302..1844
                     /locus_tag="VN496_12990"
     CDS             1302..1844
                     /locus_tag="VN496_12990"
                     /inference="COORDINATES: protein motif:HMM:NF045536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070088 - polyhydroxyalkanoate granule
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate granule-associated phasin"
                     /protein_id="HXF17917.1"
                     /translation="MNQTSNVTVLSAPILAWTDLALISAQMLVTSAQVIGHRTSRIVI
                     AGATPDPQDQREFTMMSEEKTAAALKSVQAMAQGMFRLGQQLAVMTGRQMLASVPMMM
                     SLAASATPQQSIARQTNLARAGLASAAEANHRIAASIPRIASKGLKPIHTKATANRKR
                     LHKHAVARAMNKTRAHHSGR"
     gene            2078..2545
                     /locus_tag="VN496_12995"
     CDS             2078..2545
                     /locus_tag="VN496_12995"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXF17918.1"
                     /translation="MMLALVRFANILIAALVAGGMFMIWFGYNPATLSPSTYVEQQQN
                     AIRELNVLMPVLGGITIVLTLVSAFMQRSDNKAFVLLLVASAFLILSGVLTHFGNQPI
                     NTIVLSWEIARPPANWTELRDQWWGFHRLRTFCGCVALVLITWASVYPGRRQS"
     gene            2720..4087
                     /locus_tag="VN496_13000"
     CDS             2720..4087
                     /locus_tag="VN496_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXF17919.1"
                     /translation="MTTVHIGCGAGFAGDRFDAAVPVVAALAHAKGERFLIYEVLAER
                     TLAIAQRMRRENPEQGYSPFLDTYLPLVLADCRRHGIRIVTNMGAANPLGAARRVQTL
                     AQQLGVQRIKIAAVTGDDLLATMSQDELRREPVIEGIALGKRDIVAANAYLGARSVAD
                     ALATGADVVLVGRTTDAALSLGPLLHVHGWNPDDLDRLAQGTVCGHLLECGAQVSGAY
                     FPDPGLKDVPDLARVGFPIAEVEADGSLIITKPPGTGGLVSRATVIEQLLYEVHDPHN
                     YLTPDVVLDLGEVEVEQIATNRVSVHGARGKPPPATLKATVCVDAGWLGEGEISYAGP
                     NALGRAELAASVVSARCRAIGITDPVRVEVLGTQAIFDSDARARRARVTPDETGEYRM
                     RAAVRTATRKSAQQVADEVLSLYCSGPAGGAGVRQHIGAQVATASILIDRARVKACVH
                     LVEQA"
     gene            4084..>4330
                     /locus_tag="VN496_13005"
     CDS             4084..>4330
                     /locus_tag="VN496_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17920.1"
                     /translation="MNFIDVPLHAVAHARAGDKGNRSNISVIPYLPGAFAYLVEQVTE
                     VRVLAVFAHKGATAVKRYVLPKLPAMNLVIDNALEGGV"
BASE COUNT          903 a         1232 c         1323 g          872 t
ORIGIN      
        1 ctgattgctg aagcgtcaac ctgatgctgt cgccgtacca gcgcgcgctg tgggattacg
       61 acgggtaccg ggaacgtttg caaaaacgcc cgactcttaa aaattaacac agatcgagga
      121 gagcacgcat gaaagtattc atcacgggag gaggcggctt cctcggatac cggcttgcgc
      181 tgcggctgct cgatcgcaag acattgaccg gaccggatgg caagtccgag gttatttccg
      241 aaatcaaagt atttgacgcc gcgtttgccg ccaatctcga taggcgactg cagtatatcc
      301 aggccgatat cacggatgct gatgcagtgg cgcaggcgct ggatcgcgat accgcttgca
      361 ttttccatct cgccgcggtg gtcagtgccc atgcggaagc ggagttcgac gtcggatatc
      421 gcgtaaacct cgatggcacg cgcaacttgc tggaggcatg ccgcgatctc gataggccac
      481 cgcggctggt atttgccagt tcgctggccg tattcggcgg cacgttgcca gcgatcgtcg
      541 atgatcgctg cacgccgatg ccacagactt cctatggcgt ccagaaagtg attggcgaat
      601 atctcgtcaa tgattactcg cgcaaggggt tcatcgatgg gcgcagcctg cgcctgccta
      661 ccatcgtcgt gcgtcccggc aagccaaacc ttgcatccac ttcgttcgca agcggcatgt
      721 tccgggaacc gctgaatggc gtggtatgcg aagtgccggt tcaggacaac atcgagatgt
      781 ggatcctgtc gccgggcaag gtgctggacg cgttcattca cgccaacgag ttgccggaat
      841 ctgcatgggg cacaaatcgc gcggtgactt tgccgggcat cacattttcc ctgaagcagg
      901 gtgtcgcggc gcttcgccgc attgccggtg acgaagtggc aaacaggatt acgttcaagc
      961 ctgttgatcg catcaataac atggtcaaga ccttcccggc acgcttcgac ccgcggcggg
     1021 cgctggacat gggattcaca gctgacagcg atatcgatgc catcatcaag gattacattc
     1081 aaggcgagcg catcaaagtt gcttagcggt ccggtggagc agcgtggacc agtgattgtg
     1141 attttgcagg cagtgtagta aatgagctac ctgcggcaag cgggataacg acgcaacccc
     1201 gaaattattt cttttgtgga ctgatttttc acctctgcaa aacttttccg aagattgcct
     1261 ttcgaagcgt gttccatcaa gtcatgagaa ggggacagaa aatgaatcag accagcaatg
     1321 tgacggtgtt atccgctccg attctggcgt ggaccgatct ggcattgata tcggcccaga
     1381 tgctggtgac ctcggcgcag gtcattggtc accgtaccag ccggatcgtg attgcgggcg
     1441 ccacgcccga tccgcaggat cagcgggaat tcaccatgat gagtgaagaa aaaactgccg
     1501 cagcactaaa gtcggtgcaa gccatggccc agggcatgtt caggttgggc cagcagcttg
     1561 ccgtcatgac cggcaggcaa atgcttgcca gcgtgcccat gatgatgtcg cttgccgcaa
     1621 gcgccacgcc acagcagtcc attgcacggc aaacgaatct ggcgcgagcg ggcctggcca
     1681 gtgcagcgga agcaaaccat cgaattgcgg catcgattcc ccgcatcgca agcaagggct
     1741 tgaaaccaat ccacaccaaa gcgactgcga accgcaagcg cctgcacaag catgcagtag
     1801 cccgggccat gaataaaacg cgcgcccatc actccggtcg ctagtcgaag tccgggtcaa
     1861 agcatcccgc agccctttcg agggctgctt ttttttgccc ggcttcacgg ttgtgaatac
     1921 cgccggcacc acatttccac acggaagtgc caaaacgcca aacattctgt atcttgcgcc
     1981 tatcgtaggc aggccggctg ctttctagag cgtggtcggc cgtgagaacc tgcgcgctta
     2041 aaagtagcgc tacggagaga ttcagcagga gaaaatgatg atgctcgcgc ttgtccggtt
     2101 tgccaatatc ttgattgcgg ccctggtcgc tggaggcatg ttcatgatct ggtttggcta
     2161 caatccggcg acattgtccc cttctaccta cgtggagcag cagcagaacg cgatccggga
     2221 attgaacgtg ctcatgccgg tgcttggtgg cattaccatc gtgcttaccc tggtttcagc
     2281 gttcatgcag cgaagcgaca acaaggcgtt cgttttgctg ctggttgcat ccgcattcct
     2341 gatcctcagc ggcgtgctca cgcatttcgg caatcagcct atcaatacaa tcgtgctctc
     2401 gtgggaaatc gcaaggccgc cagccaactg gactgagctg cgcgaccaat ggtggggatt
     2461 tcacaggctg aggacgttct gcggttgcgt cgcactggtg cttatcacat gggccagcgt
     2521 ctacccggga cgcaggcaaa gctgatcccg cgcagtcttg ccaatgcagg acggtgcggt
     2581 ttattcaaaa agcataaaac catgagggcg ccgaaccgga ttgcggcagg aaaagttcga
     2641 gagcgcgagc gtctcgggta aggtattgcc aatgaaaggc cgttggtgac tttgatcgcc
     2701 tgattagccg ggaaactctg tgacgactgt ccatatcggc tgcggtgccg gatttgcagg
     2761 cgaccggttt gacgcggcgg taccggtggt cgcggcgctg gcacatgcca agggcgagcg
     2821 gtttctgatc tacgaggtgc tggccgagcg cacactcgcg attgcacagc gaatgcgacg
     2881 cgaaaatccg gagcaaggtt attcgccgtt cctggacacg tatcttccgc tcgtactcgc
     2941 cgactgtcga cggcacggca ttcgcatcgt aaccaacatg ggtgccgcaa atccgctcgg
     3001 tgcagcacgc cgcgtgcaga cgctcgcgca gcaactcggc gtccagcgaa tcaaaatagc
     3061 cgctgttact ggagacgatc tgctggctac tatgtcccag gacgaattgc gtcgtgaacc
     3121 agtcatcgaa ggaattgcgc tcggtaagcg tgacatcgtg gccgccaacg cgtacctcgg
     3181 cgcacgaagc gtcgctgatg cgctcgcgac gggtgctgac gtggtgctgg tcggacgtac
     3241 cacggatgcg gcattgtcgc tcgggccgct gctgcacgtg cacggctgga atccggatga
     3301 cctggaccgg cttgcgcagg gaaccgtatg cggccatctg ctcgagtgcg gtgcacaggt
     3361 cagcggagcg tattttcccg atcccggatt gaaagacgtg cccgatctgg cgcgcgtcgg
     3421 atttccgatc gcggaagtcg aggcggacgg ctcgctgatt atcaccaagc cgcccggcac
     3481 cggcggcctg gtgagccgcg cgacagtgat cgagcaattg ctctacgagg tacacgatcc
     3541 gcacaattat ctgacgccgg atgtggtgct tgatctcggc gaagtggaag tcgaacaaat
     3601 agcgacaaac cgcgtgtcgg tacatggtgc acgcggcaag cctccgcctg ccaccctgaa
     3661 agcaacggtc tgtgtggatg ccggctggct gggggaaggc gaaatttcgt acgccggacc
     3721 caatgcgttg ggccgcgcgg aactcgccgc aagcgtggta agcgcccgtt gccgtgcgat
     3781 cggcatcacc gatccggtgc gggtggaagt gctcggtacg caagccatct tcgacagcga
     3841 cgctcgcgcc cggcgcgccc gcgtcacgcc ggatgaaacc ggcgaataca ggatgcgtgc
     3901 cgccgtgcgg acagcgaccc gcaagtccgc acagcaggtc gccgacgagg tgctgagcct
     3961 gtattgttcg gggccggcag gcggcgcggg cgtacgccag cacattggtg cgcaagtcgc
     4021 gacggcgtcc atcttgatcg accgcgcacg cgtgaaggcc tgcgtgcacc tggtggaaca
     4081 ggcatgaact tcatcgacgt tccactgcac gcggtagcac acgcccgtgc tggcgacaaa
     4141 ggcaatcgct cgaacatcag cgtcattcca tatctgcctg gcgcctttgc gtaccttgtg
     4201 gaacaggtca cggaagttcg tgtgcttgct gtgttcgcgc acaagggcgc cactgcggta
     4261 aagcgctatg tgctgccgaa attaccggcg atgaatcttg tgatcgacaa cgcgttggag
     4321 ggtggcgtca
//
LOCUS       DATMZQ010000407         4371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1545471, whole genome shotgun sequence.
ACCESSION   DATMZQ010000407 DATMZQ010000000
VERSION     DATMZQ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545471"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..791
                     /gene="urtE"
                     /locus_tag="VN496_13010"
     CDS             102..791
                     /gene="urtE"
                     /locus_tag="VN496_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010112035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HXF17921.1"
                     /translation="MLEVRNLNVYYGDSHVIRNVEFSVDDRETVAIMGRNGMGKSTLL
                     KSLIGVLPARSGQVTLDGDTMLGKKSYQRVAAGMAYVPQGRMIFPYLSVEENILAGME
                     NLKDKTVPPFVYETFPVLHEMRNRKGGNLSGGQQQQLAISRALVSNPKLLILDEPTEG
                     IQPSIIKDIARTLNKLKAERNFSLVVSEQVLSFALEIADRFLIIERGEFAYEATRDAV
                     DTAKIHAFLTV"
     gene            913..1953
                     /locus_tag="VN496_13015"
     CDS             913..1953
                     /locus_tag="VN496_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic amidase"
                     /protein_id="HXF17922.1"
                     /translation="MMHGDISSSKDGVGVAVVNYKMPRLHTKKEVLDNARKIGEMIKG
                     MKVGLPGMDLVIFPEYSTHGIMYDSKEMYDTASSCPGEETDIFAAACREAKVWGVFSL
                     TGERHEEHPNKAPYNTLILMNDKGEIVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLK
                     VSLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVIMAKAMAWANNVYVAVA
                     NAAGFDGVYSYFGHSAIIGFDGRTLGECGEEDYGIQYAALSKSLIRDARKNMQSENHL
                     FKLLHRGYTGLINSHEGNKGVAACPYDFYKKWITDPDATREMVEAITRTTVGTDEAPI
                     PGIPNKKEPAHR"
     gene            2098..2934
                     /locus_tag="VN496_13020"
     CDS             2098..2934
                     /locus_tag="VN496_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HXF17923.1"
                     /translation="MNPQDSDNLTPANGIVRQYTIDAEPYYRAIADEVPLFEAAYAVR
                     MPMMLKGPTGCGKTRFVEYMAWKLGKPLITLACNEDMTASDLVGRYLLDAQGTHWQDG
                     PLTLAVRYGAICYLDEVVEARQDTTVVIHPLTDARRVLPLEKKNELVHAHPDFQLVIS
                     YNPGYQSILKDLKQSTKQRFGALDFSYPTRDVEIEIVTHESGVGPETAAKLVNIAERA
                     RNLKGHGLEEGISTRMLIYAGSLIGQGVEPVAACHVSLIRPITDDADIRDALGATIRA
                     CF"
     gene            3077..>4371
                     /locus_tag="VN496_13025"
     CDS             3077..>4371
                     /locus_tag="VN496_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXF17924.1"
                     /translation="MAKLEEYSELLTSLGPHAREMLESSWLEATRSFTETGLTHYLEG
                     ARELATAGVGWSVVLAYLRETPGVAREVGEAAAFGAVDSALAVYAHTGAATAELVFST
                     SNIAARRLRDGGLFVAYLDFITELASLAPVGVAPILQRLDRLLEQLTLDGLRRWALLG
                     VQSHMRDPNAQARYFTLDSEEGRQLLQAEGEQTVFPEVERRLSLYLRALWGRNLKLRP
                     VIHSKGAAAGRRSTVDGWTIRMPQSYGGFGGQTGLALYRAAAAHAAAHIVLTTQRFPV
                     GSLKPLQVALVSLIEDARVEQLVMREFPGLRGLWKAFHVAQSGGAMTSGSLMARLARA
                     LIDENFEDDNPWVVKGRRLFFEQKEYWHEQSMSRALGGLLGNDLGQMRVQFNFKTYVV
                     EPAYRDDNQGIWDFGDLGESSPDDEDVVMQGARLADDHS"
BASE COUNT          950 a         1215 c         1333 g          873 t
ORIGIN      
        1 tgtgaccggt cttcctggat ctttgcggcg atcagtgccg gtgtgctgcg cgttttttcc
       61 gaaactcatg cgctggaatg ccggtggctg aggcgaaagc aatgcttgaa gtgcgcaact
      121 tgaacgtcta ttacggagac agccacgtca ttcgtaatgt tgaattcagc gttgacgaca
      181 gagaaacagt cgcgatcatg ggtcgcaatg ggatggggaa gagcacgttg ctgaagtcat
      241 tgataggtgt attgccggcg cgcagcgggc aagtcacgct tgacggcgac accatgctgg
      301 gtaagaaaag ttatcagcgc gtcgctgcgg ggatggcgta tgttccacag ggacgcatga
      361 tttttcccta cctgagcgtc gaggaaaaca tattggccgg aatggaaaat ctcaaggaca
      421 agaccgtgcc gccgttcgtc tatgaaacgt tccccgtgct gcatgaaatg cgcaaccgga
      481 agggcgggaa cctgtctggt ggccagcagc agcaactggc gatttcacgc gctttggtga
      541 gcaatccaaa gctgcttatt ttggacgagc cgaccgaagg tattcagccg tccattataa
      601 aggacatcgc gcgaacgttg aataagctta aggcggaacg caatttttcc cttgtggtct
      661 cagagcaggt cctgtcgttc gcgctcgaaa ttgcggatcg cttcctgatt atcgagcggg
      721 gagaatttgc ctacgaggct acccgggacg cggtggatac cgcaaagatt catgcctttc
      781 tgacggtttg aaacatcgga aggtccgcaa caacgaagac gggttgaagc ccgcagaaag
      841 aatcgcgcag cctacggtct gcgcaaacga gtcagcttca aacgagccaa tgatgacaag
      901 gagacaagaa tcatgatgca cggtgatatt tcaagcagca aggatggagt cggcgtagcg
      961 gtcgtcaact acaagatgcc gcggctgcac acgaagaaag aagtgctgga caacgccagg
     1021 aagatcggtg aaatgatcaa gggcatgaag gtcggcctgc cgggcatgga tctcgtcatt
     1081 tttccggaat acagcaccca tggaatcatg tacgacagca aggaaatgta cgataccgct
     1141 tcgagttgtc ctggcgagga aaccgacatc tttgccgccg catgccgcga agccaaggtc
     1201 tggggcgtgt tctccctgac cggtgaacgg cacgaggaac atccgaacaa ggccccctac
     1261 aacacgctca ttctcatgaa cgacaagggt gagatcgtgc agaagtaccg caagatcatg
     1321 ccgtgggtgc cgatcgaagg ctggtatccc ggcaactgca cctacgtttc cgaaggtccg
     1381 aaaggcctta aggtttcgct gattatttgc gatgacggca actaccccga aatttggcgt
     1441 gactgcgcca tgaagggcgc cgaactgatc gtgcgctgtc aaggctacat gtatccggca
     1501 aaggaccagc aagtcatcat ggcgaaagcc atggcctggg ccaacaacgt ttacgttgcg
     1561 gtagcgaacg cggccggttt cgatggcgtg tattcgtact tcggccactc cgcgatcatc
     1621 ggtttcgatg gtcgcacgct cggcgaatgc ggcgaagagg actacggcat ccagtatgcg
     1681 gcactgtcca agtcactgat ccgcgatgcg cgaaaaaaca tgcaatccga aaaccatctg
     1741 ttcaagctgc tgcaccgcgg ttacaccggc ctgatcaact cgcacgaggg caacaaaggc
     1801 gtggcggcat gcccgtacga tttctataag aagtggatta ccgatccgga tgccacccgc
     1861 gaaatggtgg aagccattac gcgcaccacg gtcggcacgg acgaagcgcc tatccccgga
     1921 attcccaaca agaaggaacc tgcgcaccgg taacatccgc tgcaaaccaa ctggccgggc
     1981 tggcgtctta cgcgagtccg gtcaggtgct attcaaatca aggcaccatc ggcgcggcat
     2041 tcggttccaa tcaagaagac atgacatctg cgtgcaacgc cgcgaaggaa agcaaccatg
     2101 aatccgcagg acagcgataa cctgacgccc gccaacggca tcgtgcgtca atacaccata
     2161 gacgcggagc cgtattaccg cgcgattgcc gatgaagtgc ctttgttcga agcggcatac
     2221 gccgtacgca tgcccatgat gctgaaaggg ccgaccggct gcggtaagac gcgtttcgtg
     2281 gaatacatgg cctggaagct cggcaagccg ctgatcacgc tcgcgtgtaa cgaagatatg
     2341 accgcatccg atctggtggg ccgctatctg ttggacgcgc aaggtacgca ttggcaggat
     2401 ggcccgctaa ccctggcggt gcgctatggc gcgatttgct atctcgacga agtcgtggag
     2461 gcacgccagg acaccacggt cgtgattcac ccgctgaccg atgcgcgccg cgtgctgccg
     2521 ctggaaaaaa agaacgagct ggtgcatgcg catccggatt tccagctggt aatttcctac
     2581 aaccccgggt atcagagcat cttgaaggat ctgaagcagt ccaccaagca gcgctttggc
     2641 gcgctggatt tcagctatcc gacgcgcgac gtggaaattg agattgtcac ccacgaatcg
     2701 ggtgtcgggc cggaaaccgc tgccaagctg gtgaacatcg ccgagcgcgc ccgcaatctg
     2761 aagggccacg gtctggaaga aggaatatcc acccgcatgc tgatttatgc aggctcgctg
     2821 atcggccagg gtgtcgaacc ggtggcggcc tgccatgtct cattgatccg gcctatcacg
     2881 gacgatgcgg atattcgcga tgccttgggc gcgacgattc gtgcctgctt ttgaattttg
     2941 gctcggtaca aagtaaggcg ccttcgaata gtacaaaatg gccatgcgag ttggatgctt
     3001 cctgccccaa ccacaattgt gattctcccc cgtttccttc gtgttctccg tggttcagtc
     3061 tggaattgca tgaatcatgg cgaagcttga ggagtacagc gagctactta cgtcgctcgg
     3121 gccccacgcg cgggaaatgc tggaatcgag ctggctcgaa gcgacccgct cgttcaccga
     3181 aaccggcctg actcattatc tggaaggtgc tcgcgagctg gcgacagccg gcgtcgggtg
     3241 gagcgtcgtg ctggcgtatc tgcgtgagac gcctggcgtt gcacgcgaag tcggcgaggc
     3301 ggccgccttt ggcgcggtcg attcagcgct cgcggtgtac gcgcacaccg gtgctgccac
     3361 cgcggagctg gtgttctcca ccagtaatat cgccgcgcgc aggttgcgcg atggcgggct
     3421 gtttgtcgcg tatctggatt tcatcactga actggcgagc cttgccccgg tcggtgtcgc
     3481 cccgatcctg cagcggctgg atcgcctgct cgaacagttg accctggacg gattgcgccg
     3541 ctgggccctg ctaggcgtgc agtcccacat gcgcgatccc aatgcgcagg cgcgctactt
     3601 cacactcgat agtgaagaag gccgccaact gttgcaggcg gaaggcgaac agaccgtgtt
     3661 ccccgaagtc gagcgccggt tgtcgctcta cctgcgcgcc ttgtggggtc gcaacctgaa
     3721 gttgaggcct gtcatccact ccaaaggagc cgccgcggga cggcgctcta ccgtcgatgg
     3781 ctggaccatc cgcatgccgc agtcctacgg cggctttggc ggccaaactg gtctcgcgct
     3841 gtatcgcgcc gcggcggctc atgccgctgc ccacattgtt ctaaccacgc agcgctttcc
     3901 cgtgggcagt ctcaagccgt tgcaggtggc gctggtttct ctgatcgagg atgcacgcgt
     3961 cgagcagctt gtcatgcgcg aatttcccgg cctgcgcgga ttgtggaagg cctttcacgt
     4021 ggcgcagtct gggggggcca tgacatcggg ttccctgatg gcgcgcctgg cgcgggcact
     4081 gatcgacgag aacttcgaag atgacaatcc gtgggtcgtc aaaggccggc gcctgttctt
     4141 cgagcagaag gagtattggc acgagcaatc catgagccgc gcactcggtg gattgctcgg
     4201 caatgacctg ggccagatgc gggtgcagtt caacttcaag acttacgtgg tggaaccggc
     4261 gtaccgggac gacaatcaag ggatctggga tttcggcgac cttggagagt cctcgccgga
     4321 cgacgaagat gttgtgatgc agggcgcgcg gctggctgac gaccattcca a
//
LOCUS       DATMZQ010000408         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4123390, whole genome shotgun sequence.
ACCESSION   DATMZQ010000408 DATMZQ010000000
VERSION     DATMZQ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4123390"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VN496_13030"
     CDS             <1..856
                     /locus_tag="VN496_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17925.1"
                     /translation="TASVKAGPLRRHDQELLSGVTPRIDGDDFSLGISGRRGLLQYFL
                     FKNTNVLYDQPQVHVLPSRASATFPETMTWTLDHPIATSEGNGDLTILSQGHYSNLIG
                     SYRTTISREGDITISADFSYAGPDALTKEIGFQFDVPLALDRLSWQRKGDWTWYPEDH
                     IGALSGDVQAHSGKPAFTVPTWPFAEDDSPMGSNMYRSTKRNIISAMVRDEAGHGWMI
                     RSDGSQHLRASVGTDRISVFVSDWYGGSTADMGEYVENYGDGKMLHTGERIHTTLRLS
                     PLRGGDAP"
     gene            875..1915
                     /locus_tag="VN496_13035"
     CDS             875..1915
                     /locus_tag="VN496_13035"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXF17926.1"
                     /translation="MFRIVELLSLAAYSVWGGQKRTPNLRTHSQTTRGDPATTMLRLN
                     GITPVMATCFREDETIDDDSMRKQIDFAIEAGAAAICGPGFGAEFYKLSDEERFHFVD
                     VLVEHTRKRVPAIAATSSGSTYSTIEFSKYAEKIGADCVMVTAPRTTPLPAPEIISYY
                     SRLCDSLKIPVMLQDADFTGAGLPAKVFVDLAHKHPNFRFAKLEVTLPGRKSAEIVEQ
                     TKGQVQIIYGLGGIAMIDGLAHGASAVMPGAACLEVYVRVYELYTQGKKDRAEALFQR
                     LIPYLAFALQHLELAIYIEKRVMHKRVILPNSRMRQPSLSFDSVYQEQIDRLVNSAVA
                     LCEECRVATVKK"
BASE COUNT          465 a          577 c          608 g          459 t
ORIGIN      
        1 aactgcctcg gtgaaagcag ggccactgcg ccggcatgat caggaactgc tgtccggcgt
       61 tactccgcgc attgatggag acgacttttc actggggatt tccgggcggc gtggattact
      121 gcagtatttt cttttcaaga acacgaacgt tctttatgac cagccccagg ttcatgtact
      181 tccatcccgt gcttccgcca cgttccccga gactatgaca tggacactcg accaccccat
      241 tgcgacctcc gagggcaatg gcgacctgac aatcctgagc cagggacatt attccaacct
      301 gatcgggagc tatcgaacca ctatctcgcg ggagggagac atcacgattt cagcggactt
      361 ctcctatgca ggacccgatg cgttgacgaa agaaatcggg ttccaattcg acgtgcccct
      421 ggctcttgat cgcttgtcgt ggcagcggaa gggcgactgg acctggtatc ccgaagacca
      481 tatcggcgcg ctgtcagggg atgtccaggc acatagcgga aagccggcat tcaccgttcc
      541 cacgtggcct tttgccgagg atgattcgcc catgggcagc aatatgtatc gcagcactaa
      601 acggaacata atttcggcga tggtaagaga tgaagctggt cacgggtgga tgatccgctc
      661 cgatgggtcg cagcacctac gcgcgtcggt gggcacggac cgcattagcg tgtttgtgag
      721 cgattggtac gggggaagca cggccgatat gggggaatac gtagagaatt acggcgacgg
      781 caagatgttg cacaccggcg agcgcattca cactacattg cggctcagcc cgttgcgggg
      841 cggcgacgca ccatagtatg tacggattcg gaacatgttc cgaattgttg aattattgtc
      901 tctcgccgcg tatagtgtct ggggcggcca gaagcgaacg ccgaatcttc gcacccattc
      961 acagaccacc aggggagatc cagcaacgac catgttaaga ctcaacggca ttacgccagt
     1021 gatggcaact tgcttccgag aagacgaaac cattgacgac gactcgatgc gcaaacagat
     1081 cgatttcgca atcgaggcag gagcggcagc aatttgtggt cctggatttg gcgctgagtt
     1141 ttataagctg tctgacgagg aaagattcca cttcgtggac gtattggttg agcatacccg
     1201 caagcgggtg ccggcgattg cggccaccag ttcgggctcg acctacagca cgatcgagtt
     1261 ctccaagtat gcggagaaga ttggcgccga ctgcgtgatg gtgaccgctc cgcgtaccac
     1321 gcctcttccc gcgccggaaa tcatcagcta ctacagccgg ctgtgcgatt cgctgaagat
     1381 cccggtcatg ctgcaggatg cggacttcac tggcgctggg cttccggcca aagtgttcgt
     1441 tgacctggca cacaagcatc cgaatttccg ttttgccaaa ctcgaggtca ctctgccggg
     1501 acgaaagtca gccgagattg ttgagcagac caaggggcaa gtacagatca tttacggact
     1561 aggcggcatc gcgatgatcg atgggctggc gcatggcgcg tcagcagtaa tgccgggagc
     1621 ggcctgcctt gaagtttacg tccgcgttta tgaactttat actcaaggaa agaaagaccg
     1681 agccgaggca ctgtttcagc gcctgatccc ttatcttgca tttgccttac agcacctgga
     1741 actcgccatt tatattgaga agcgggtcat gcacaagcgc gtgatactgc ccaactcccg
     1801 gatgcgccaa ccgtcgctct cgttcgattc tgtttatcag gagcagatcg accggctggt
     1861 aaattccgcg gtcgcgttat gcgaagaatg ccgggtcgca accgtcaaaa aatagatcga
     1921 cgctgtaagg agtcctcatt gcgctcattg tttattgatg ccattcttgc gctctgcttg
     1981 ctggcgccgc cgccaagtgc ttcggcgaaa cagaaaacgc tccgcatcac atttgtagac
     2041 gtggaagggg gtcaggcgac actgctggtg gcgccttccg gcgcttccat gctggtcgac
     2101 accggctgg
//
LOCUS       DATMZQ010000409         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1363197, whole genome shotgun sequence.
ACCESSION   DATMZQ010000409 DATMZQ010000000
VERSION     DATMZQ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363197"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VN496_13040"
     CDS             <1..293
                     /locus_tag="VN496_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HXF17927.1"
                     /translation="GFWGYFFPLSVTMSTSMCYELIEWGAAAILGGDVGQAYLGTQGD
                     VWDAQKDMAFAGSGALIAMLVTVGMNVRFERDFVGESNNSLRVKPARPLRKD"
     gene            complement(321..1307)
                     /locus_tag="VN496_13045"
     CDS             complement(321..1307)
                     /locus_tag="VN496_13045"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXF17928.1"
                     /translation="MSEDQIEHAARILREGGLVAFPTETVYGLGADASNPAAIRKLFA
                     AKGRPADHPVIVHVANTSDLKHWTAEVPRGAWVLAERFWPGPLTMVFKRANQVSDLVT
                     GGQDTVGLRVPSHPLAQRLLRAFGGGIAAPSANRFGHVSPTTAQHVRDEFGDTVDLVI
                     DGGPCDVGIESTIVDMSHGKPAVLRPGRITAQQIADALSVPPLPDSAIGRPRVSGSLN
                     SHYAPRLPLTILQQEEIEDYLRARASTPVAVLSRRGRPADSKAVLWQVAPETADAYAR
                     LLYDTLRRLDVSGCRMILVEALPELPEWAAVRDRVGRAATPEPVASTTTTRG"
     gene            complement(1325..2212)
                     /locus_tag="VN496_13050"
     CDS             complement(1325..2212)
                     /locus_tag="VN496_13050"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXF17929.1"
                     /translation="MSTHKPLYETSLKSLKFMHRGKVRDLYEIDADRMLIIQTDRLSA
                     FDVVLPTPIPNKGRVLTAMSNFWFRKLGHVLPNHLLDVAPESVVAPAERAQVEGRAFV
                     VRKLKPLPIEAIVRGYITGSGWKDYQKTQMICGIPLPAGLQEAQQLPQPLFTPSTKAA
                     LGAHDENISFAQMERLIDPALAGRVRDKTIQLYSEAAAYAATRRIIIADTKFEFGQDR
                     AGALYLIDEALTPDSSRFWPKDQYRVGTSPPSFDKQFVRDWLEARKWNKQPPAPDLPD
                     DVLRKTSEKYEEALRLLTG"
     gene            complement(2238..2759)
                     /gene="purE"
                     /locus_tag="VN496_13055"
     CDS             complement(2238..2759)
                     /gene="purE"
                     /locus_tag="VN496_13055"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXF17930.1"
                     /translation="MSNKTVSDSRKHATVAIVMGSTSDWDVMQHAARQLKDFGVPFEA
                     RALSAHRTPHLLADFVEQQVAGGTKCFIAGAGGAAHLAGVVAALTILPVLAVPIPSKH
                     LGGLDSLLSMVQMPKGIPVATFAIGDGGAANAGLFAVSMLALTDTQLSGRLQAFRVKQ
                     SDAVKAATLPELS"
BASE COUNT          565 a          828 c          810 g          585 t
ORIGIN      
        1 gcggcttctg gggctatttc tttccattga gcgtcaccat gtccacatcc atgtgttacg
       61 aattaatcga gtggggcgcg gcagcgattt tgggcggcga tgtcggtcag gcctaccttg
      121 gtacacaagg ggacgtctgg gatgcgcaaa aagatatggc atttgccggt agcggcgcac
      181 tgatcgccat gctggttacc gtcggcatga acgtccggtt cgagcgggat tttgtgggcg
      241 aatcgaataa cagtttgcgc gtcaaacctg cccgaccgct ccgcaaagat taagctccgc
      301 gggaaacgat tgtcttgtaa tcagccacgc gtggtggttg tcgacgcaac cggttccggg
      361 gttgcggctc tccccacccg gtcacgcacg gctgcccatt caggcagttc aggcaacgct
      421 tccacgagga tcattcggca gccggacaca tccaaacggc gcaacgtatc gtagagcagg
      481 cgcgcgtatg catcggcggt ttccggtgcg acttgccaca aaaccgcttt cgaatccgcc
      541 ggccgtccgc ggcgcgacag cacggcgacc ggtgtgctgg cgcgggccct taaataatct
      601 tcgatctctt cctgctgtag gatcgtgagc ggcagtcgcg gcgcatagtg cgaattcagc
      661 gagcccgaca cgcgcgggcg cccgattgca gagtccggca ggggcggtac gcttaacgca
      721 tcggcaattt gctgggcggt gatacggccc gggcgcaata ccgcgggttt tccatggctc
      781 atatcgacaa tggtcgattc gattccgacg tcgcacgggc caccatcgat cacgaggtcc
      841 accgtgtccc caaattcgtc acgcacgtgc tgcgccgtcg taggagaaac atgcccaaac
      901 cggtttgcag acggggcggc aatgccgccg ccgaacgctc tcagcaatcg ctgcgcaagc
      961 ggatgcgaag gaacacgcag gcctacggtg tcctgtcctc ccgtaaccag atcactgacc
     1021 tggttggctc gcttaaatac catggtcagt ggacccggcc agaacctttc cgcgaggacc
     1081 catgcgccac gcggaacctc cgccgtccaa tgtttcagat cactggtatt ggcgacatgc
     1141 acgatcaccg ggtggtcggc aggacgcccc ttcgccgcaa acagtttccg gatcgctgcc
     1201 ggattcgacg catcggcacc cagcccgtat accgtctcgg ttggaaaagc caccagcccg
     1261 ccttcgcgca ggatgcgcgc agcgtgctcg atctggtctt cactcatgac gtccggtttg
     1321 cgttttaacc cgttaacagg cgcagcgcct cttcatattt ttcgctggtt ttgcgcaaca
     1381 cgtcatcggg caaatcgggc gcgggcggct gcttgttcca tttgcgtgcc tccagccaat
     1441 cgcgcacaaa ctgcttgtcg aaggagggcg gactcgttcc aacacgatat tggtccttcg
     1501 gccagaaacg agaagagtcc ggcgtcaatg cttcatcgat cagatacaac gccccggccc
     1561 tgtcctgccc gaactcgaac ttggtgtccg cgataatgat gcggcgcgtt gcggcgtatg
     1621 ccgcggcttc agagtagagc tggatggtct tgtcccgtac ccggccagcc agcgcagggt
     1681 cgatcaaccg ttccatttgg gcaaacgaaa tattttcgtc gtgagcgccg agagcagctt
     1741 tggttgaagg cgtgaaaagc ggctgtggca attgctgcgc ctcctgaaga cccgcaggca
     1801 acgggatgcc gcagatcatt tgtgttttct ggtagtcctt ccagcctgaa cccgttatgt
     1861 aaccgcgcac aattgcctcg attggcagcg gcttgagctt ccgcaccacg aatgcgcgac
     1921 cttccacctg tgcgcgttcc gccggcgcca ccacggattc gggcgccaca tcgagaagat
     1981 ggttcggtag tacatgaccc aatttacgga accagaaatt ggacatcgcc gtcagtacgc
     2041 gtcctttatt gggtattggc gtaggcaaca caacgtcgaa cgcggaaagt cgatcggtct
     2101 ggataatcaa catgcggtct gcatcaatct cgtagagatc ccgcaccttc ccgcgatgca
     2161 tgaatttgag cgacttgaga ctggtttcgt agaggggctt atgcgtactc atggttggcg
     2221 tccggcacgc gtgaaattca ggataattcc ggcagggtcg ctgcttttac cgcatcggat
     2281 tgcttcaccc ggaacgcttg caggcggccg gatagttgcg tatccgttag cgcaagcata
     2341 gagaccgcaa acaatcccgc gttggcagcg ccgccatcgc cgattgcaaa ggttgctaca
     2401 ggtatgccct ttggcatctg caccatcgac agcaacgagt ccagaccgcc gagatgtttg
     2461 gaaggaatcg gcacagcaag cacaggcaaa atggtcagtg ccgcaaccac accggcaaga
     2521 tgggccgcgc caccggctcc ggcaatgaag cacttcgtac caccggcgac ctgctgctcg
     2581 acaaaatcag ccagcagatg cggcgtacgg tgcgcggata aggcgcgtgc ctcgaatgga
     2641 acgccgaagt ccttgagttg acgtgctgca tgctgcatta catcccagtc gctggtgctt
     2701 cccatgacaa tagcgaccgt cgcatgtttc ctgctatcag atacagtttt gtttgacatt
     2761 gtagagctcc tgcctggttt acgatgcg
//
LOCUS       DATMZQ010000410         4528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3617500, whole genome shotgun sequence.
ACCESSION   DATMZQ010000410 DATMZQ010000000
VERSION     DATMZQ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617500"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /locus_tag="VN496_13060"
     CDS             <1..1949
                     /locus_tag="VN496_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HXF17931.1"
                     /translation="TLALAELYPWLRSQDAEAAPLRELTTVSGTSAVTLHNLTGSVKR
                     PAELRYDLTIEPKQITATYDGLPGPVSIMGGTVRVDPKNIKIDHLDVSLLDAKATLAA
                     TIANYQGKQLQATASASGGSIGQQSMGWVWTRTGAPQRLLPKTPLRFPTAQVTWGPQR
                     ALDAKVALQMEGERSVAVELSWQPDALAVRRVHIKDALSNAMLSFELKERLLQASFSG
                     LLSADSVASLFNEQGQYPGVIQGDMRFTLDLARQGRARAEGHLGAQGLDLTGLLAQPV
                     VIEQLALDADGATLHIRQAAVNWAQQLINISGDVKRGEHGAVIDAQIDSAGIVLDALL
                     PQKETAEQGPEAKTPEPPAAQKGEFFPTLWPLPVVGQIKIRSGFIQYQKHRIEPLSAA
                     MTLEENRAGINVDEAISCGISLPLTVEVSPQQVTASARIRAANQELGETMRCLSDERV
                     LITGTFDAQVDLTTSGKREDLLRNLKGNVHAEARNGRIMKFGLIGNILSVKAVTSLFG
                     KDGPRFDSEGFTYRKLVTRARIEGGKIFIDEGALDSDALGLAATGSVSLLDRQSKLTV
                     LVAPFSRVDRIMRKIPIVGYIFGGLLTTLPVGVSGDIRDPLVVPLGPSAVTSQLVGVF
                     TRTLEVPEKLLAPVTGGSQQKKQP"
     gene            complement(1963..2976)
                     /locus_tag="VN496_13065"
     CDS             complement(1963..2976)
                     /locus_tag="VN496_13065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXF17932.1"
                     /translation="MKAMIITDAWSPQVNGVVTTLGKTGEGLKRLGHEVRFVTPQLFK
                     SIPCPTYPEIRLSLMPYGGVARQIMEFSPDAIHIATEGPLGMAARRYCLKNGMPFTSA
                     FHTRFAEYVHARIRVPVDLTYRWLRNFHAPSQALMVPTPSVQRELRSRGFNNTVAWTR
                     GVDTVTFSPGARDGLAGRRPIFLYVGRVAVEKNVEAFLKLNLPGSKWVVGEGPMRAHL
                     ERKYPDVYFAGIKTPAELARIYRAADVFVFPSLTDTFGLVLLEALACGTPVAAFPVLG
                     PIDVIGDSGAGVIHDDLRVACMQALEISREKARAYAERFSWQISVEQFVRHLVPMKLL
                     SAA"
     gene            complement(2973..3758)
                     /locus_tag="VN496_13070"
                     /pseudo
     CDS             complement(2973..3758)
                     /locus_tag="VN496_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
     gene            4372..>4528
                     /locus_tag="VN496_13075"
     CDS             4372..>4528
                     /locus_tag="VN496_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXF17933.1"
                     /translation="MMAEQTVDYALFALDAAGMVATWNRGAQRIKGYAPEEIIGRHFS
                     VFYTREAI"
BASE COUNT          990 a         1271 c         1343 g          924 t
ORIGIN      
        1 tcacgcttgc actggcggaa ttgtatccct ggctgcgatc gcaagacgca gaggccgcac
       61 cactgcgaga actgacgacg gtatcaggaa cgtctgcagt tacgctgcac aatctgactg
      121 gcagtgtaaa acggcccgca gagcttagat atgacctaac catcgagccc aaacagatca
      181 ctgcgacgta cgacggccta ccggggccag tgagcattat gggcggaacc gttcgcgtcg
      241 atccgaagaa catcaagatt gatcatctcg acgtctcatt gctggacgct aaagctacgc
      301 tcgccgcgac gatcgcgaat tatcaaggca aacaactgca ggcgactgcg agcgcttccg
      361 gcggttcgat tggacagcag tcgatggggt gggtgtggac gcgcaccgga gcaccgcagc
      421 gcctgctgcc gaaaaccccg ttgcgatttc cgacagccca ggtgacctgg ggtccgcagc
      481 gcgcattgga tgcgaaggtg gcgttgcaaa tggaaggcga gcgcagcgta gcagtggagc
      541 tttcgtggca acccgatgcg ctcgcggtgc gacgcgttca cataaaggat gcactgagca
      601 acgcgatgct ctcgttcgag ctcaaggagc gcctgttgca agcttccttc tccggcttgc
      661 tgagcgccga cagcgttgcg tcgttgttca atgagcaggg gcagtatccc ggtgtgatac
      721 agggcgacat gcgcttcacc ctggacttgg cgcgccaggg acgcgcgcgt gcggaaggtc
      781 atctcggcgc acagggactt gatctgaccg gcctgctcgc gcagccggtg gtaattgagc
      841 agctcgcact ggacgcagac ggggccaccc tgcatatccg gcaagcagca gtgaactggg
      901 cgcagcaact gatcaatatc agcggcgacg tgaaacgggg agaacacggt gccgttatcg
      961 atgcacaaat tgattcggct ggcattgtgc tggatgcact gctgccgcaa aaggagactg
     1021 cagagcaggg gccagaggca aaaacacccg aacctccggc cgcccagaag ggagaatttt
     1081 ttccgacgct gtggccgctg ccggtggttg ggcaaatcaa aatacgttcc ggatttattc
     1141 aataccagaa gcatcgtatt gaacccttgt ccgcggccat gacactggaa gaaaaccgtg
     1201 cggggatcaa cgtggatgaa gcaatctcgt gcggtatttc gcttccgctg acggtggagg
     1261 tttcgccgca acaagtcacg gcttcagctc gcattcgcgc agccaaccag gagctcggag
     1321 aaaccatgcg ttgcttgagc gacgagcgtg tactgattac tggcacgttc gacgctcaag
     1381 ttgacctgac gaccagcggc aagcgcgaag atttgctgcg gaatctgaag ggcaacgtgc
     1441 acgccgaggc acgtaacggt cgtatcatga aattcggcct gatcggcaat attctttcgg
     1501 taaaggccgt caccagcctg tttggcaagg acggaccgcg cttcgacagt gaaggtttca
     1561 cttaccgcaa gctggtgaca cgcgcacgta tcgagggcgg aaagatcttc atcgacgagg
     1621 gagcgctgga cagcgatgca cttggcctcg ccgccaccgg gtcggtcagc ttgctggatc
     1681 ggcagtccaa gctcaccgtg ctggtggcac ccttcagccg ggttgaccgc atcatgcgca
     1741 agattccgat tgtgggctat atctttggag gcttgttgac cacgcttccg gttggtgtca
     1801 gcggagacat tcgtgatccg ctggtggtcc cgctaggccc gagtgcggtc acgtcgcaac
     1861 tggttggcgt tttcaccaga acactggagg ttccggagaa gctgcttgca ccggtgaccg
     1921 gcggttcgca gcaaaaaaaa caaccatgag tttcggtcgc cgctatgcgg ctgacagcag
     1981 tttcatcggc acaagatgcc ggacaaactg ctcaacggag atctgccagg aaaatcgctc
     2041 cgcgtaggcg cgcgcctttt cccgcgagat ttccaaggcc tgcatgcagg cgacgcgcag
     2101 atcatcgtgg atcacccccg caccgctgtc accaattacg tcgatcggcc ccagcaccgg
     2161 gaaagccgcc accggcgttc cgcacgccag ggcttccagc aggacgagtc cgaacgtgtc
     2221 ggtcaggctg ggaaatacaa aaacatcggc cgcgcgatag attctcgcca attccgcggg
     2281 cgtcttgata cccgcaaagt agacatcggg atacttgcgc tccagatggg ctcgcatcgg
     2341 tccttcgccc accacccatt tgctgcctgg caagttcagt ttcagaaagg cttcgacgtt
     2401 tttttccact gcgacacggc cgacataaag gaaaattgga cgccgtcccg cgaggccatc
     2461 gcgcgcgccg ggagaaaacg ttacggtgtc cacgccgcgc gtccatgcga cggtattgtt
     2521 gaatccacgc gaccgcaatt cacgctgaac gcttggagtc ggaaccatga gtgcctgcga
     2581 aggtgcatga aaattgcgca gccaccggta cgtcaggtcg accgggacgc gtatccgtgc
     2641 atgcacatac tctgcaaagc gcgtgtgaaa tgccgaagtg aacggcatgc cgttctttag
     2701 acagtagcgg cgcgcagcca tgccaagcgg accttccgtg gcgatatgga tggcatcagg
     2761 agaaaattcc ataatctgcc gggcgacgcc gccataaggc atgaggctaa ggcggatttc
     2821 cgggtaggtg gggcacggaa tggatttgaa cagctgcgga gtcacgaacc tcacctcgtg
     2881 accgagtcgc ttcaagcctt caccagtttt accgagtgta gtgaccacgc cgttcacttg
     2941 tggagaccat gcgtctgtga tgatcattgc cttcatgcct cgatgtactc ctcttgtcgt
     3001 tcgatgatcg aattggaagg tgcggtccgg tgcaggcgct gccactggac aatttttagt
     3061 tcgccctcgt aggtttctac cagcgcggac aggctttcta cccagtcgcc gtcgttgcag
     3121 tagaggatgc cgtcgatgct cttgatctgc gctttgtgga tgtgtccgca aatcacgccg
     3181 tgaaaaccgc gctggcgcgc ttcccgggct acggcgtctt cgaacgccgt catgaaattc
     3241 accgcatttt tggccttgtg cttcaggtat tgcgaaagcg accaataacc caggtcgagc
     3301 cgcgcacgag cacggttgaa ccaatggttc aatttcagga tcagcacgta gagttgatcg
     3361 ccaagcaacg ccagccactt ggcttgctgg accaccgcat cgtagagatc gccgtgtacg
     3421 actagaaatt tgcggccgtc ggccatggtg tggacggctt catcgcatat ttcaatgtca
     3481 ccgaatgcca ggccgaggaa ctgacgcagg acttcgtcgt ggttaccggc gatgtaggtg
     3541 acttttgtgc cgcggcgcgc tttgcgcaaa actttctgga cgacgtcgtt gtgcgcctgc
     3601 ggccaatacc agccgcgctt cagctgccag ccgtcgatga tatcgccgac aagatacagt
     3661 tggtccgatt cgtggtgccg tagaaaatcg agcaggtatt cggcctggca gccgggagtt
     3721 cccaaatgga tatcagagat ccagatcgaa cgataccgct gtacgcctgg tgcgggcgga
     3781 gtgccgaaac tcagaacgtc agtctctgtg ccatgatggc gcgcattcaa tcacgcccat
     3841 gtgacactgg tattgcaagc acgtgctctg tgtgcaaaag cctgggcggt attccgcgga
     3901 cccgccttgt tcgtcattcg caacacttgc gaagcatgga aggcccggtt gatggaccaa
     3961 gcttgccggg ctagaattgc gcgttttgcg aagcccggcg cgacgaaaca gttgcgcgct
     4021 ggttcgacaa ttggggaagt cgagtagccc gtgattcccg tctgacgtat cggcttgaat
     4081 ctgactcctg gctgatagca aacggcgttt acgctgaaca aaacgaggtc tgctgcttct
     4141 aagcccattc gaccgcctgg tatatgcgct gtcctcagtg aaacaactga aggccgcggc
     4201 gagtggagtg gctacagatt tgaacaccgt tccattacaa caatattgcg tgctcccacg
     4261 gtggggcgta gattccccgc ctacgagtgt tggcgacaaa aaaacaaaca ataaccaagt
     4321 ttaggcatgg ctgagaacaa caataccgcg ataagccccg ccatctatca aatgatggct
     4381 gaacagacgg tggattatgc gttattcgcg cttgacgccg cgggtatggt agcgacatgg
     4441 aaccgcggtg ctcagcgcat caaaggctac gccccagagg aaatcatcgg gcgccatttt
     4501 tccgttttct atactcgcga ggcgatcg
//
LOCUS       DATMZQ010000411         3431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_328661, whole genome shotgun sequence.
ACCESSION   DATMZQ010000411 DATMZQ010000000
VERSION     DATMZQ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328661"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VN496_13080"
     CDS             <1..309
                     /locus_tag="VN496_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HXF17934.1"
                     /translation="GVIALQTALRQPRRLAGAMSLSAYLPLVHKIEQERNAANNDMPV
                     FLGHGTSDNVVPLVLGKASRDQLIKMGYDVDWHQYPMAHSVCAEELEDLSGWFNRVLS
                     "
     gene            complement(329..1771)
                     /locus_tag="VN496_13085"
     CDS             complement(329..1771)
                     /locus_tag="VN496_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17935.1"
                     /translation="MSMQDSVKVLGEFGSRLGVAQRRRPELKLGAKIELVDRADRGAV
                     ELLQALETWYLKDLNADAGERTHVWDAISQYCTQLERAYLYLARQFQTYNHGWAEVRD
                     WIPVVVARAIRASSLRVKWQLMRYLPIEKDIWQTLARLWCFAEDKGMEQARVMVYEDK
                     STLAQEFLRPMMLAISAADSLPALEVDIAFRVIGHFSDRFELQRHPSKACNFFVDIDR
                     WTPPERYTSLAVMRSGARFFGAGRVMQQIDDLISKVAAGEILPKDVNLETVDDMGAVI
                     HVLEHLGRHWWERRPERRTERRRSLSQIGVVYGFDEIMGRLSSEDTTDLLPPVETWNV
                     ENESDDGYGAVLPMGQGDWLQVGRFIALKPVDTRIWEIGIVRRLGAQEAGTRNIGIQL
                     LARGARPITLQRRTDTQQTWQALLLPAHSGGDASTREVNLLLPVGTFLPDASMQMEVY
                     STRYVLEPRVLLEHSQEFEMARYRIVQRVQ"
     gene            complement(1842..3377)
                     /locus_tag="VN496_13090"
     CDS             complement(1842..3377)
                     /locus_tag="VN496_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17936.1"
                     /translation="MLKWIKGADSEHPLADDKEARQYIAELPPLDPYKSLQELCYWLD
                     MLEGADEIKLTRVLEIVDLVDLTARNYQRKLSQDYLGSSARLQKFQEIRIWNAVSTFW
                     KALANAYHLCVVRYQGKAGGSGAVKPQMPMVVGRALRALTLQLKWQLLRYGPVEPRIW
                     LQMGKLFAYAEQSEFATTPVIVYPGGSGESTVQREFLKGLMLNISSTDSLLPAKLEIA
                     DRVVARFAESFVISRQPAKGCHYYFDLGAGKLPARVMSRLQPTPGVRFFGPGNAAQQL
                     EKLIGTIQADGAVPSHINLGGSFDTESVLETLRHLARYWAPSPPARGEERRKAVSRIS
                     VVHDFDEILSTVSGETQDLSFDSSVELWTVENESDNGYGAIVPLASADWLRVGTLLGV
                     RLEDGASWGVGIVRRFAAYDEKQRYVGIQVLTKGATVVKLATVNSAPSEGESALLLPS
                     NNNETSGASEINLLLRPGTFSLQKSMEMRVYERTYLLVPRKLIEAGYDFDMTRFRVMQ
                     RAS"
BASE COUNT          699 a         1074 c          947 g          711 t
ORIGIN      
        1 ggcgtgatcg cactgcaaac cgctctgcgc cagccgcgcc gtctagcggg ggcgatgtcg
       61 ctctcggctt acctgccgct cgttcacaaa atcgaacagg aacgcaatgc cgcaaataac
      121 gacatgccgg tgttccttgg ccatggcacc tcggacaacg tcgtgccgct cgtactgggc
      181 aaggcatccc gcgaccaatt gatcaagatg ggatacgacg tcgactggca ccagtacccg
      241 atggcacact cggtttgcgc ggaagaactc gaggatttga gcggatggtt caaccgcgtg
      301 ctgagttgaa ggcccgaatt ctgtctcgct attggacacg ttgcacgata cggtagcgcg
      361 ccatttcgaa ttcctggctg tgctccagca agacacgcgg ttcgagtacg tagcgcgtgc
      421 tgtagacctc catctgcata ctggcatcgg gcaggaacgt acccaccggc aataacaaat
      481 tcacttctct cgtgctcgcg tcaccgccgg aatgcgcggg caacagcagt gcttgccagg
      541 tttgctgcgt gtcggttctg cgctgtagcg taattggccg cgcgccgcgg gcaagcaatt
      601 gaatgccgat gttacgggtg cccgcttcct gcgcgccgag ccggcgcaca atgccgatct
      661 cccaaatgcg cgtatcgacc ggtttcaagg caatgaacct gccaacctgc aaccagtcgc
      721 cttgtcccat cggcagcacg gcaccgtaac cgtcgtcgct ctcgttctcc acgttccacg
      781 tttcaaccgg cggcagcaag tccgtcgtat cttcggaaga caggcgcccc atgatttcgt
      841 cgaatccgta cacgacgcct atttgcgaca gtgagcgtcg gcgttccgtg cgccgttccg
      901 gccgccgctc ccaccaatgc cgcccgagat gctcgagcac atgaatcact gcccccatat
      961 catcgaccgt ttcgaggttc acgtccttgg gcaggatttc accagctgcc acttttgaga
     1021 tgaggtcgtc gatttgctgc atgacccggc cggcaccgaa aaatcgcgcg cccgagcgca
     1081 ttacagcgag cgaggtgtag cgctccggag gcgtccagcg atcgatgtcg acgaaaaaat
     1141 tgcaagcctt ggatggatgc cgctgcagtt cgaatcgatc cgaaaaatgt ccgatgacgc
     1201 gaaatgcaat atcgacttcc agcgctggca ggctgtccgc ggccgagatc gccagcatca
     1261 tcggtcgcag gaactcctgc gccagagtgg acttgtcctc gtagaccatt acgcgcgcct
     1321 gctccatgcc tttgtcttcg gcgaagcacc acaatctggc aagggtctgc cagatgtctt
     1381 tttcaatcgg cagatagcgc atgagctgcc acttaacgcg caatgagctt gcgcggattg
     1441 cgcgcgccac gactaccgga atccagtcac ggacttcggc ccagccatgg ttgtaggtct
     1501 ggaattgccg cgccaggtac aggtatgccc gttccagctg cgtgcaatat tgcgaaatag
     1561 cgtcccagac gtgggttcgc tcgccggcat cggcattaag gtctttcaaa taccaggtct
     1621 ccaatgcctg cagcaactcc accgcaccgc ggtccgcacg gtccaccagc tctatcttgg
     1681 cgccgagctt cagttcgggg cgccgccgct gcgcgacgcc gagcctggag ccaaactcac
     1741 ccagcacctt gacgctatcc tgcattgaca ttgaagctag tgagagacca gaaaacaagc
     1801 atgaacggta ttcttttcca ggatagacac gcgcattgcg ctcagcttgc gcgctgcatg
     1861 acccggaagc gcgtcatatc gaaatcgtat ccggcttcaa tcaactttcg cggcaccagc
     1921 aggtacgtac gctcgtacac gcgcatttcc atgcttttct gcaacgagaa agtacctggc
     1981 cggagcagca gattaatttc cgacgcgccg ctggtttcgt tgttgttcga tggcagcagc
     2041 agcgcgcttt ccccctcgga cggagcgcta ttgacggtag caagctttac tacggtggcg
     2101 cctttggtca ggacctgaat gccaacgtag cgctgtttct cgtcataggc ggcaaacctg
     2161 cgcacgatgc cgacccccca ggacgccccg tcctccaacc tcacgcccaa cagcgtaccc
     2221 acgcgcagcc agtcggcgct ggcaagcggc acgatcgctc catagccgtt atcgctctcg
     2281 ttttcgaccg tccacaactc gaccgaactg tcgaaggaca ggtcctgagt ttcgccggaa
     2341 acggtagaca aaatttcatc gaaatcgtga accacgctga tgcgcgaaac tgctttgcgt
     2401 ctctcttcgc cgcgcgcagg aggagagggc gcccaatagc gcgccaggtg acgcaaggtt
     2461 tccagaactg attcggtgtc gaacgatccg ccgagattga tgtgcgacgg gacagcacca
     2521 tcagcctgaa tcgttccaat gagtttttcc agttgctgtg ctgcatttcc aggaccgaag
     2581 aaccgcacgc cgggcgttgg ttgcaggcgc gacatcacgc gcgccggcaa cttgcccgcc
     2641 ccgagatcga aataataatg acagcccttc gcgggctgcc ggctgattac gaaagattcg
     2701 gcgaaccgcg cgacgactcg gtccgcgatt tcgagcttgg ctggcagcag gctgtcggta
     2761 gaggagatat tcagcatcag gcccttgagg aattctcgtt gcacggtgga ctctccagag
     2821 ccccccgggt aaacgatgac cggcgtggtg gcaaattcgg attgttctgc ataagcgaac
     2881 agcttgccca tctgcagcca gattctcggc tcgaccggtc cgtaccgcag cagctgccac
     2941 ttcaattgca acgtcaacgc ccttagcgcc cgccccacca ccatgggcat ctgcggcttg
     3001 actgcacccg acccgccggc tttgccctgg tagcgcacca cgcacaaatg gtacgcattg
     3061 gcgagcgctt tccagaatgt ggacactgca ttccagatgc gtatttcctg gaatttctgc
     3121 agccgtgcgc tgctgcccag ataatcctgc gacagtttgc gttggtaatt cctggccgtc
     3181 agatcgacca gatcgacgat ttccagcacg cgcgtcaact tgatttcatc cgcgccttcc
     3241 agcatatcga gccagtagca cagttcctgg agcgatttgt aaggatccag cggcggcagt
     3301 tccgcgatgt actggcgtgc ctccttgtcg tcggccagcg gatgttccga atcggcaccc
     3361 ttgatccact tgagcatgca aaagtcccct tatggtccgg aggttatcgg ccggagcgga
     3421 ttatatcccg c
//
LOCUS       DATMZQ010000412         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_675279, whole genome shotgun sequence.
ACCESSION   DATMZQ010000412 DATMZQ010000000
VERSION     DATMZQ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675279"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2285
                     /locus_tag="VN496_13095"
     CDS             <1..2285
                     /locus_tag="VN496_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF17937.1"
                     /translation="AELPKMQAGLPPDLVTKIATDQSIFVRAAVDGVINEAILAGCLT
                     ALMILLFLGSWRSTLIIAVTIPLAILTSLIALYALGETINIMTLGGLALAVGILVDDA
                     TVAIENISQHLESGKPLDQAILDGAQQIAIPTFVSTLAICIVFVPMFLLTGVARYLFV
                     PMAEAVVFAMLASYVFSRTLVPTLAKYLLRAGLLDEHGHPRQSRNPFVRFHHAFEQGF
                     EGVRDNYHGFLERCMHHQKFFMVVFLAGCLLSGALLPFVGRDFFPSVDAGQFKLHVRA
                     KTGTRIEETARLCDLVENEIRRIIPPHELATIIDNIGLPLSGTNMSYNMSGTLGPADA
                     DIQVSLNAGHSPTERYMRQLRQRLPEQFPGLTFYFLPADIVSQILNFGLPAPIDVQVV
                     GRNLDANRAFTLGLYQKLKQVDGIADLRVQQPFDAPRMNIEVDRTLAQQAGFSQKDVA
                     SNLLIALSGSFQTSPSFWLNPKNGVSYNIITQTPQYNIDSLQALANIPISGPSSTRSS
                     ILGDLATVTRGSERAVGSHWNVQPVMDIFGATQGRDLGAVADDVNKIVEGSMKDLPKG
                     SRLIVRGQIETMLSSFQGLLYGLIFAIVMVYLLMVVNFQSWLDPFIIITALPAALAGI
                     VWMLFATGTTVSVPALTGAIMAMGIATANSILVVSFARERLQAGHDSVTASLEAGFSR
                     FRPVLMTALAMIIGMIPMSLGLGEGGEQNAPLGRAVIGGLLLATVATLFFVPVVFAFI
                     HGFIARRRDAAERRQSPQPA"
BASE COUNT          467 a          690 c          710 g          543 t
ORIGIN      
        1 aagcggaatt accgaagatg caggccggcc tgccgcccga tctggtgacc aagatagcaa
       61 ctgaccaatc gatattcgta cgcgcggccg tggatggggt cattaatgaa gcgattctcg
      121 ccgggtgcct gaccgcgctg atgattttgc ttttcctggg cagctggcgc agcacgctga
      181 tcatcgcggt gaccattccg ctcgccatcc tgacctcgct cattgcgctc tacgcgcttg
      241 gcgagaccat caacatcatg acgcttggcg ggctggcgct cgcagtcggt attctggttg
      301 acgatgcaac ggtggccatc gagaacatca gccagcatct ggagagcggc aagccgttgg
      361 atcaggcgat tctggacggc gcgcagcaga tcgccattcc gacatttgta tccacactgg
      421 cgatctgtat cgtattcgtg ccaatgttcc tgctcaccgg cgtggcgcga tacctgtttg
      481 tgccaatggc ggaggcggtt gtgttcgcca tgctggcctc atacgtcttc tcacggacac
      541 tggtccccac gctggccaaa tatctgctgc gcgcagggct gctcgatgaa cacggccatc
      601 cgcggcaaag ccgcaatccg ttcgtgcgtt ttcaccacgc ctttgagcag ggttttgaag
      661 gtgtccgcga caactatcac ggctttctgg aacgctgcat gcaccaccag aaatttttca
      721 tggttgtgtt tcttgccggc tgtttgctct ccggcgcgtt gttgccattc gtcggccgcg
      781 attttttccc gagcgtggat gcggggcagt tcaagcttca tgtacgcgcc aagactggca
      841 cccgtatcga ggagaccgcg cgtctgtgcg atctggtgga aaacgaaata cgcaggatca
      901 tcccgcctca tgaacttgcc accattatcg acaatatcgg gctgccgctc tccggcacca
      961 acatgtcata caacatgtcc ggcacgcttg ggccggccga tgccgacatt caggtttcgc
     1021 tgaatgcggg gcacagtccg accgagcgct acatgaggca actgcggcag cgcctgccgg
     1081 aacaatttcc cggtttgacc ttttatttct tgcccgccga tatcgtaagt cagattctga
     1141 attttggcct gcctgcgccg atcgacgtgc aagtggtcgg gcgcaatctc gacgccaacc
     1201 gcgcgttcac attaggtctg tatcagaaac tcaagcaggt ggacggcatt gcggacctgc
     1261 gtgtgcagca gcctttcgat gcgccaagaa tgaacatcga agtggacagg acgctggcgc
     1321 agcaggctgg attctcccag aaggacgttg cctcgaacct gctgattgcg ttgagcggaa
     1381 gttttcagac cagcccgtcg ttctggctca atccaaaaaa cggagtgagc tacaacatca
     1441 ttacgcagac gccgcaatac aacatcgatt ccctgcaggc gctggcgaac attccgattt
     1501 ccggtcccag ttccacgcgc tccagcattc tgggcgatct cgcgaccgtg acgcgcggtt
     1561 ccgagcgtgc agtcggcagc cactggaatg tgcaaccggt aatggacatc ttcggagcga
     1621 cccaagggcg cgatctgggt gctgtagccg acgatgtgaa caagatcgtt gaaggcagca
     1681 tgaaagattt gccgaagggc tcgcgcctga tcgtgcgtgg tcagattgaa accatgctgt
     1741 cctcattcca ggggttgctg tacggtttga tatttgcaat tgtcatggtt tacttgttga
     1801 tggtcgtgaa ttttcagtcc tggttggatc ctttcatcat catcaccgcc ttgccagcgg
     1861 cgctggcagg cattgtctgg atgctgtttg cgacaggcac tactgtcagc gtgccagcgc
     1921 tgacaggggc catcatggca atgggtatcg caacagcgaa cagtattctg gtggtcagtt
     1981 tcgcgcggga acgcctgcag gccggccatg attccgtgac agcttcgctt gaagccgggt
     2041 tcagccggtt ccgtccggta ctcatgacgg cacttgccat gatcatcggc atgatcccga
     2101 tgtcgctcgg cttgggtgag ggcggcgagc aaaatgcgcc gctgggccgg gccgtcatcg
     2161 gcggcctgct gctcgccacg gttgccaccc tgtttttcgt gccggtggta ttcgcattca
     2221 ttcatggctt catagcgcgc cgccgcgacg ccgcggagcg ccggcaaagc ccgcaaccgg
     2281 cataagtttt caaaggtaga catgacagtg caagagccag gttataagga caagcaaatt
     2341 ggcagcgaac gcacagcggg tgccttgcgc ccggtagccg gtcgtagagt atcgcgccgg
     2401 tggtgggtgc
//
LOCUS       DATMZQ010000413        13036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2746868, whole genome shotgun sequence.
ACCESSION   DATMZQ010000413 DATMZQ010000000
VERSION     DATMZQ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746868"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..819
                     /locus_tag="VN496_13100"
     CDS             148..819
                     /locus_tag="VN496_13100"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HXF17938.1"
                     /translation="MLELYTSEGCNSCPPADRWLSSLPARGLTSERLIPLAFHVDYWD
                     QLGWPDRMAKEQFSARQRTQSQRNGNTVVYTPQFLLNGIDYRIDPRIEQRINKLNGLP
                     PAAELVARQHQTSSGFELEIDARLMGKYEDPVQTFVAITEDRLESAIKAGENQGKLLH
                     HDFVVRELWKPIAKDPLGDVHWRSATMLAADWKRADLSLVVFVQNQRNGEVLQALKTP
                     LCAGK"
     gene            1071..2657
                     /locus_tag="VN496_13105"
     CDS             1071..2657
                     /locus_tag="VN496_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HXF17939.1"
                     /translation="MSEFNWDFPYSSRRMPVLARNIVATSQPLAAQAGLRMLFRGGNA
                     IDAAIATAAALTVVEPVMNGIGSDAFALIWNGQSLHGLNASGRAPKAWTPDRFAGRER
                     MPTEGWDSVTVPGVVSAWAALSRKFGKLDFASLFEPAIDYARDGFPVSPVVSMQWASQ
                     IERLKDQPGFAQAFARDGQAPKAGQIFRFPDQAETLTEIARTGGESFYRGDLARRIAK
                     FSAECGGALGMEDLAAHSAEWVEPVRQRYRGYCVHEIPPNGQGIAALIGLGILEHFDM
                     GSITRDSVESLHLQIEAMKLGFADVYRHVADANAMRFNASNMLDPAYLKSRAQLVRRD
                     RASQAVAGTPPSGTIYLTTADASGMMVSYIQSNYMGFGSGVVVPGTGISMQNRGYGFS
                     LAPGHPNVVGGGKRPFHTIIPAFLTRDNEPVMSFGVMGADMQPQGHMQMVVRIIDYRQ
                     NPQAAADAPRWKIMLDGGLLLEHAVSDEVARGLAQLGHRVVRASLGSTEFGAAQLIYR
                     LGDIYVGASEPRRDGQAVGF"
     gene            complement(2759..4444)
                     /locus_tag="VN496_13110"
     CDS             complement(2759..4444)
                     /locus_tag="VN496_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXF17940.1"
                     /translation="MIQNMPLRSGGRILVDQLAVHGADTAFCVPGESFLDVLNAFYDA
                     RERMRLVVCRQEGGAAFMAEAYGKLTGRPGLCFVTRGPGATNASIGVHTAYQDSTPMI
                     LFIGQVGSDVVEREAFQEMDYRRMFGQMAKWVAQIDRADRIPEYVSHAFHTAVSGRPG
                     PVVLSLPEDMLVSQAAAMDAAPYTKVKAHPGVADMDRLRELLDQARRPLVILGGGDWS
                     LSACANIRSFIESNNLPATCSFRRQDLLDNRHPNYCGDVGIGINPALAERVRTADLIL
                     AVGARLGEMTSGGYTLFAIPRPLQRIIHIHPSAEELGRVYQANLMINSGMQEFAEAAL
                     GIPPVDSSQWRDWTHQAREDYVRNLKHGSMPGALDLGEVMAFLRKRLPENAIITNGAG
                     NYSGWIQRFYQYTGFRTQLAPTNGAMGYGVPAGIAAKIVHPERMVVSFSGDGCFTMNG
                     QELATAMQYELPLLFIVVNNAMYGTIRMHQERAYPARVYATALKNPDFAALAKAYGLH
                     GEVVAKTAEFEPAFERAMASNKPALLELRIDPEAITTRTTLTAVREQAIKAGH"
     gene            4620..6332
                     /locus_tag="VN496_13115"
     CDS             4620..6332
                     /locus_tag="VN496_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HXF17941.1"
                     /translation="MAWSQIYDPMNNAVLSTALAALPVIVLLGGLAFFRLSAHVAALL
                     GLAAALIIAIFVYGMPAEMAGASAAYGAFFGLLPIGWIVLNIIFLYQLTKDKGYFQIL
                     QDSIASVTEDRRLQLLLIAFAFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLSL
                     IANTAPVAYGALGTPIIALSAVTGLDLLQLSAMVGRQLPFFSLLVPFWLIWAFAGWRG
                     MREIWPAILVTGVSFAIPQFLISNFHGPWLVDVGAAIISMACLTLFLRVWKPKTIWRT
                     TALKGHREEGSAAAGAQPVQARKHDRAAVMRAWTPWIVLSVFVFLWGIPQVKILLNGG
                     DAKAKIEYAWNGYTKIDVPVPGLNDMVQKVPPVVAKPSTEKAVYSLNWLSATGSGILI
                     AALIAGFYMRYSFTDLLRKYWQTIVLVRFSLLTIAAMLALGFTTRYSGTDATMGLAFA
                     HTGALYPFFGTLLGWLGVALTGSDTASNVLFGGLQKITSEQLGLNPNLMAAANSSGGV
                     MGKMIDAQSIVVASTATRWYGHEGDILRYVFSHSIVLAMLVGGLVMLQAYVFPFMQVM
                     PIAP"
     gene            complement(6390..8471)
                     /gene="pnp"
                     /locus_tag="VN496_13120"
     CDS             complement(6390..8471)
                     /gene="pnp"
                     /locus_tag="VN496_13120"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXF17942.1"
                     /translation="MNYGRHSLTLETGEIARQAHGSVVVNMDDTVVLVTVVGRKDVKP
                     GQDFFPLTVDYQERTYAAGKIPGGFFKREGRPSEKEVLTSRLIDRPIRPLFPEGFYNE
                     IQVIATVVSSNNEVDSDIPAMIGASAALAISGIPFDGPIGAARVGYLNGEYVLNPTAT
                     ELKTSQLNLVVAGTSQAVLMVESEALELPEEVMLGAVVFGHQQMQLVINAINELADEV
                     GKPLWELPPPQKESALVERIAAIAEAELRQAYSLRQKQARTEKIDAVRGKVLTDAAPD
                     NSDTAQVNLVKSIFSDIEARIVRTQILNGEPRIDGRDTRTVRPITIRAGVLPRAHGSA
                     LFTRGETQALAVATLGTARDEQIIDALQGEYSERFMLHYNFPPYSTGETGRVGTPKRR
                     EVGHGRLAKRALLAVLPAPEEFSYSMRVVSEITESNGSSSMASVCGGCLALMDAGVPL
                     KAHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTENGVTALQMDIKIQGIT
                     KEIMNVALHQAKEGRLHILSIMRETLSTARSELSAYAPRMITMKIKPEKIRDVIGKGG
                     AVIRAITEETGTTIDINDDGTVTIACVSSEGGEAAKRRIEEITAEVEVGRIYDGTVLK
                     LLDFGAIVSVLPGKDGLLHISQIANERVNSVADYLKEGQAVRVKVLEADEKGRLRLSM
                     KAVAEEQKAAS"
     gene            complement(8635..8904)
                     /gene="rpsO"
                     /locus_tag="VN496_13125"
     CDS             complement(8635..8904)
                     /gene="rpsO"
                     /locus_tag="VN496_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXF17943.1"
                     /translation="MSISTSQKAQILQDHQRAKADTGSPEVQVALLTARINDLTGHFK
                     DHVKDHHSRRGLLRMVSRRRKLLDYLRRSDRDRYRTLLDRLGLRK"
     gene            complement(8975..9883)
                     /gene="truB"
                     /locus_tag="VN496_13130"
     CDS             complement(8975..9883)
                     /gene="truB"
                     /locus_tag="VN496_13130"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXF17944.1"
                     /translation="MDHGVLLLDKPAGLSSNVALQRVRRLFSWSKAGHTGTLDPLATG
                     LLPICLGEATKFSHALLDADKAYEASIRLGLVSSTGDAEGILEACAEPNFTASRLENV
                     LASFIGVTEQVPPMHSALKKHGTPLYKYARAGKTVVREPRKIIIKRLQAIDVTSHTIT
                     ITVDCSKGTYIRVLAEDIGKALGCGGYLGSLRRTGIRDLTVTNAIDLESLTEMNESSR
                     RARLLPVDRLVEFLPALHLPAETTRRIINGLGVPSAGGGPGVFRLYDLAGNFIGIGES
                     TNDGLLAAKRLVSQVRKPSEKPAALD"
     gene            complement(9913..10287)
                     /gene="rbfA"
                     /locus_tag="VN496_13135"
     CDS             complement(9913..10287)
                     /gene="rbfA"
                     /locus_tag="VN496_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXF17945.1"
                     /translation="MAKNPSRLARIADQIQKDLAELIRTQVKDPRVVMLTLTGVEISS
                     DYHYAKVFFTTLGGPESVARTIGGLEHASGFLRSQLAKGLKLRIVPELRFIYDESVER
                     GVRISTLIDDAIASHPGEEGKP"
     gene            complement(10360..12936)
                     /gene="infB"
                     /locus_tag="VN496_13140"
     CDS             complement(10360..12936)
                     /gene="infB"
                     /locus_tag="VN496_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXF17946.1"
                     /translation="MAQMNVAQFAKELGLASAMLLEQLQAAGIGKSLADDTVLTEHDK
                     TQLLDYLRRVHGAKDTKNKITLTRRQTSEIKKSDSTGKARTIQVEVKKKRVLVKRDTA
                     SETAENVVHDVDLAPPPAPATPATPPNVNAEQTALREEEARKQAELIARQTAEAAQKQ
                     QRKRKSTKEAEPPVVQAEPVAVAPVAKAAEEPKKITTEGTLHKPAAKPSEKAAKPKPA
                     KKPDAWKDESAKRRGLKTRGDVTGGAQSWHTRSGRGGRHKTETVAPEQTFTAPDPVIR
                     EVLVPETITVAALAQKMSVKAAEVIKVLMKLGSMVTINQVIDQETAMIVVQEMGHIAK
                     PAKLDDPESFLADEGEKHDVVAEPRAPVVTVMGHVDHGKTSLLDYIRRTRVASGEAGG
                     ITQHIGAYHVNTPRGMITFLDTPGHEAFTAMRARGAKVTDLVVLVVAADDGVMPQTLE
                     AVNHAKAAKVPVLVAVNKIDKPEANPEKIRQELAAHEVVPEEWGGDAMFQDVSAKTGQ
                     GIDELLERILLQAEVMELKAPKDAPARGVVIESKLDKGRGPVATILVQSGTLKRGDVV
                     LAGAVFGRVRAMLDEAGHQIQEGGPSIPVEIQGLSDVPLAGEEMIALGDERKAREIAL
                     FRQGKFREVKLAKQQTAKLEGVYDQMTTGQAKALSLIIKADVQGSYEALSQSLEKLST
                     AEVKVNIVHAGVGAITESDVNLALASNAVIIGFNTRADATARKLVAAHGIDLRYYNII
                     YEAVDEVKSALSGMLAPEKKENILGLVEIRQVFKISKVGTVAGCYVLEGLIRRGSLIR
                     VLRDNVVAHTGELDSLKRFKDDVREVKAGFECGLSVKNFDDLKVGDQVEAYEVVEVAR
                     SL"
BASE COUNT         2636 a         3678 c         3600 g         3122 t
ORIGIN      
        1 aaacctttac ggccaggaag ctcttcatcc atgaaaccaa ctcgcctatt gcgcattgtt
       61 ttactcatcg taggttcttg tgtcgaaccg cttgcctttt cggcaaacgc gccgtgcgag
      121 gcgcgcagtt cggccagtcg ggtagcggtg ctcgaacttt atacctctga aggttgcaac
      181 agctgccccc ctgctgatcg ctggctgagc tcattgccgg ctcgtggatt gacttccgag
      241 cgcctgatac cgctggcctt tcatgtcgat tactgggatc agctgggctg gcccgatcgc
      301 atggcaaaag agcagttcag cgcacgtcag cgcacacagt cacagcgcaa tggcaatacc
      361 gtcgtctaca caccacagtt cctgcttaat ggcattgatt accgaatcga ccctcgcatc
      421 gagcaacgga tcaacaaact caacgggctg ccgcctgccg cagaactggt cgcgcgtcag
      481 catcagacct cttcaggatt tgaacttgag attgatgcac ggctgatggg caaatacgaa
      541 gacccggtac agacatttgt tgcaatcact gaagaccgcc tggagagcgc aatcaaggcc
      601 ggagagaatc aaggcaaact acttcatcat gactttgttg ttcgcgaact ctggaagccg
      661 atagcgaaag atcccttggg tgacgtgcat tggagatcag caacgatgct ggctgcagat
      721 tggaagcgcg ctgatctgtc acttgttgtt ttcgtacaga atcagcgaaa tggcgaagtt
      781 ctgcaagcgc tgaagacgcc attgtgtgcc ggtaaatgac accgacggtg cactggggtt
      841 tgcgaatacc ggaaggcctg tgaaagaata gcgccgccca tcgatcaatt cctggttgca
      901 ctgcacgggt cacgcctgca tacgcccgcc aatcacaaca acacagggaa tacggacggg
      961 ctcaagcatg gacggatgcg cggcggacat agcgaagctt gccattgcgg ctgtcgcatg
     1021 cgatccgcca ctgcgaataa gaatcaggca ggccgaacag gagtgaagta atgagtgaat
     1081 tcaactggga ctttccgtat tcgtcccgtc gtatgcccgt gttggcacga aatatcgtgg
     1141 ctacttctca acctctggct gcccaggcag gccttcgcat gctgtttcga gggggcaatg
     1201 caattgatgc cgctattgca actgctgccg ctctgacggt cgttgagccg gtcatgaatg
     1261 gcatcggctc ggatgcgttt gcgctgatat ggaacgggca atcattgcat ggactgaatg
     1321 catcaggccg tgcgccaaaa gcttggactc ctgaccggtt cgccgggcgg gagcgcatgc
     1381 caacggaagg atgggacagt gtcacggtac ccggggtggt gtctgcatgg gcggctttat
     1441 cccgcaaatt cggcaagctt gatttcgcat ccctgttcga gccggccatt gactatgccc
     1501 gcgatggttt tcccgtctca ccggtggtgt ccatgcaatg ggcatcgcag atcgagcggc
     1561 tgaaagatca gcctggcttt gcgcaggcgt tcgcgcgtga tggccaggcg ccaaaggcag
     1621 gccagatatt tcgttttccg gatcaggcgg agaccctgac ggagatagcg cgcaccggcg
     1681 gcgaatcgtt ctatcgcgga gatctggcga ggagaattgc aaagttcagt gccgagtgtg
     1741 gcggggcgct cggtatggaa gacttggccg ctcatagcgc ggagtgggtt gaacccgtgc
     1801 gccagcgata tcgaggttac tgcgtgcacg agataccgcc caatggtcag ggtatcgccg
     1861 cgctgatcgg attgggcatt ctcgagcatt tcgatatggg cagtatcacg cgggattctg
     1921 tcgaaagctt gcacctgcag atcgaggcaa tgaaactcgg attcgcggat gtgtaccgcc
     1981 atgtggcgga tgcaaacgca atgcgcttca atgcatccaa catgctagac ccggcatacc
     2041 tgaaatcgcg cgcgcaattg gtacggcggg atcgtgccag tcaggcggtt gccggcactc
     2101 cgccctcggg caccatttat ctcaccactg cggatgccag tgggatgatg gtttcgtaca
     2161 ttcagtccaa ctacatggga ttcggatcgg gcgtcgtagt gccgggcacg ggaatcagca
     2221 tgcagaatcg cggatacgga ttttcgctgg ctcccggaca tcccaacgtc gtaggcggtg
     2281 gaaagcggcc ctttcatacg atcattccgg cattccttac acgcgataat gagcccgtca
     2341 tgagttttgg cgtgatgggt gccgatatgc aaccgcaggg acatatgcag atggtggtcc
     2401 gcatcatcga ttacaggcaa aatccgcaag ccgcggccga tgcgccgcgt tggaaaatca
     2461 tgctcgatgg cgggttgctg ctggagcatg ctgtttctga tgaagtggcg cgagggctgg
     2521 cacagctggg tcaccgggtt gtgcgtgcgt cgcttggcag tacggaattt ggcgcagcac
     2581 agctaatcta tcggctgggc gatatttacg tcggggcttc agaacccagg cgcgatggac
     2641 aggcggttgg cttttagtca acgggatcgc ccgtcggtgt aacggttgcc cgcttcacgg
     2701 tggaacttcc tgtgcggaca aagcgatcag acattcgaaa ccgggttgag caatttaatt
     2761 agtgaccggc cttgatagct tgctcgcgta cggcggtcag tgtagtgcgc gttgtaattg
     2821 cttccggatc gatacgcaat tccagcaacg ctggtttatt gctggccatt gcacgctcga
     2881 aagccggctc gaactcggcg gtcttggcta caacttcgcc gtgtagccca taagctttag
     2941 caagcgctgc gaaatcagga tttttcagcg cggtcgcata gacccgggcc ggataggccc
     3001 gctcctgatg catgcgtatg gtcccgtaca tcgcattatt cacgacgata aaaagcagcg
     3061 gtaattcata ttgcatggcc gtagccaact cctgcccgtt catcgtgaag cagccatcac
     3121 ctgaaaaact gacgaccatg cgctccgggt gaacgatttt tgcggcaatg cccgcgggca
     3181 cgccgtaacc catggcgccg ttggtaggcg ctagttgggt gcgaaagccg gtgtattgat
     3241 aaaagcgctg tatccacccg gaataattgc cggcgccatt ggtaatgatc gcgttttcag
     3301 gcagacgttt gcgcaggaat gccatcactt ctccaagatc cagtgcgccc ggcattgatc
     3361 catgctttag attcctgacg taatcctcac gtgcttgatg tgtccaatcg cgccactgcg
     3421 aagaatcgac aggcggaatg ccaagcgcgg cttcggcaaa ttcctgcatg cccgagttga
     3481 tcatgagatt tgcctggtaa acccgtccga gttcttccgc tgacggatga atatggataa
     3541 tgcgctgtag cgggcgggga atggcaaaca gcgtgtagcc accgctcgtc atttctccga
     3601 gacgagctcc gacagcgaga atcagatcgg cggtgcgcac ccgttccgcc aacgcgggat
     3661 tgattccaat tccgacatcg ccgcaatagt tcggatgccg gttgtccagc agatcctgtc
     3721 tgcggaaact gcacgtcgct ggcagattgt ttgattcgat gaagcttcga atattggcgc
     3781 aggcactcaa cgaccagtcc ccgccgccca aaatcaccag cggtctgcgc gcctggtcca
     3841 gtaattcccg cagccgatcc atgtctgcaa cgccggggtg cgctttaact ttcgtgtagg
     3901 gtgctgcgtc catcgccgcg gcctggctta ccagcatatc ctcgggcaat gagagcacga
     3961 ctggacctgg tcttcccgaa actgccgtat gaaaagcgtg gctcacatat tctggaattc
     4021 tgtccgcccg atcgatttga gcaacccatt ttgccatttg gccaaacatg cggcggtaat
     4081 ccatctcctg gaaggcttcg cgctcaacca cgtcactgcc aacctgcccg atgaacagaa
     4141 tcatgggcgt agagtcctga tacgccgtat gcacgccgat acttgcattt gttgcaccag
     4201 gccctcttgt gacgaagcac agtccgggac gaccggtaag ctttccgtat gcttcagcca
     4261 taaaagctgc accgccttct tgccggcata cgaccaaacg catacgttcg cgggcgtcgt
     4321 aaaaggcgtt cagcacatcc agaaagctct cccccggtac acagaaagcc gtgtccgcgc
     4381 catgaaccgc aagctgatcc accagaattc gtccgccgct tctcaatggc atgttttgaa
     4441 tcatgcatgc tctcctctgc tcacgcgctg aattatacgc atgcatcaat tcttgccgtg
     4501 cttcgcgaat gacattttgc aggttccgtt tggatataat ggcgcgcgtc tcgatatcaa
     4561 aaaacgcccg tcttcggtct gcttctggac cgtacgggac acataaggga ggtatgctca
     4621 tggcctggtc gcagatctac gatccgatga acaatgccgt gctcagcacg gcacttgccg
     4681 cactacctgt catagtcctt ctcggcggtc tcgcgttctt ccggttgtcc gcgcacgtgg
     4741 cagccctgtt gggattggct gcggcgctca tcattgcgat ctttgtttat ggcatgcccg
     4801 cagaaatggc cggtgcatcg gctgcctatg gtgcattttt tggattgctg ccgatcggct
     4861 ggatcgtact gaacataata tttttgtatc agctcacgaa ggacaagggc tatttccaga
     4921 ttttgcaaga cagcattgcc agtgtcaccg aagaccgccg tctgcaactc ctgctcatcg
     4981 cgtttgcatt cggtgctttt tttgaaggag ccgccggctt cgggacgccg gtcgccgtga
     5041 ccggtgccat tctgatcggc ctgggatttt ccccattggc tgcatccggt ctttctctca
     5101 ttgccaatac ggcgccggtt gcctatggcg cgctgggcac gccgattatt gcgctgtccg
     5161 ctgttaccgg gctggacctg ctgcagttat cagcgatggt cggccgccaa ttgccgttct
     5221 tttcgctgct ggtgccattt tggctgatct gggccttcgc gggctggcgt ggcatgagag
     5281 aaatctggcc ggccattttg gtaaccggtg tcagcttcgc gatcccgcaa ttcctcattt
     5341 cgaattttca tggcccgtgg ttggtggatg tcggcgcagc cattatttca atggcatgcc
     5401 tgacactatt tctgcgtgta tggaaaccga aaaccatatg gagaaccacg gcactcaagg
     5461 gacacaggga agaaggctct gctgctgctg gcgctcaacc cgtgcaagcc agaaaacatg
     5521 atcgtgcggc ggtaatgcgg gcctggacgc cgtggatcgt cttgtcagtg ttcgttttcc
     5581 tgtggggaat accgcaagta aagattctgc tcaacggcgg cgatgcaaag gcgaaaatag
     5641 aatatgcgtg gaacggctac accaagatcg acgtcccggt tccggggctc aatgacatgg
     5701 tgcagaaagt gcctccggta gtcgcgaagc cttccaccga aaaagcggtt tacagcctga
     5761 actggttatc tgcaaccgga tccggaattc tgattgcggc actgattgct ggcttttata
     5821 tgcgctattc gttcacggat ctgctgcgca agtactggca gacaatcgtg ctggtgagat
     5881 tttcactgct gacgattgcc gcaatgctgg ccctgggctt tacgacccgc tattcgggaa
     5941 cggatgccac catgggattg gcgtttgctc ataccggggc gctgtatccg ttcttcggca
     6001 cattactggg atggctcggg gtggcgttga ccggatccga tacggcttcc aacgtgctgt
     6061 ttggcgggtt gcaaaaaatc acgtcggaac agcttggcct gaatccgaat ctcatggcgg
     6121 cggcaaatag ctccggcggc gtcatgggta agatgatcga tgcgcagtcc attgtcgtgg
     6181 catcgacggc cactcgctgg tacgggcatg aaggcgacat actgcggtac gtgttctccc
     6241 acagcatcgt gctcgccatg ctcgttggcg ggctggtcat gctgcaggca tatgtatttc
     6301 cgttcatgca ggtaatgccc atcgctccat aacgggtgaa cgtaaaaaaa agcccaccat
     6361 caggtgggct tttttgttac tggaccggct caactggctg ctttttgttc ttcggcaacc
     6421 gccttcatgg acagtcgcag ccggcctttt tcgtccgctt cgagtacttt gacgcgcaca
     6481 gcctgacctt ccttcaaata atcggcaacg ctattgaccc gttcattggc aatctgcgaa
     6541 atgtgcagga gtccatcttt accgggcagg acgctgacaa ttgcaccgaa gtccaggagc
     6601 tttaataccg tgccgtcata aatgcgcccg acttcgacct ccgccgtaat ttcctcgatg
     6661 cggcgtttgg ctgcttcgcc gccctctgac gatacgcacg caatagtcac ggtgccatcg
     6721 tcattgatat cgatcgttgt tcctgtttct tcagtaatgg ctctaatgac tgcaccgccc
     6781 ttgccgatca cgtcccgaat tttttcgggt ttgatcttca tggttatcat gcgcggcgcg
     6841 tacgcagaca attcggaacg cgctgtagac agcgtctctc tcatgatact caggatgtgc
     6901 aggcgacctt ccttggcctg gtgcaaggcg acgttcatga tctctttggt aatgccctgg
     6961 attttgatgt ccatctgcag ggcggtaacg ccgttctccg taccggctac cttgaaatcc
     7021 atgtcaccaa ggtgatcttc atcgccaaga atatctgtaa gcacagcaaa gcgattgcct
     7081 tccttgatca gtcccatcgc aatacccgcg acgtgcgctt tgagcggcac gcccgcatcc
     7141 atcagcgcca ggcaaccacc gcacactgag gccatcgaac ttgaaccatt ggattccgtg
     7201 atttccgaca cgacgcgcat cgaataagaa aattcctcgg gcgccggcaa taccgctagc
     7261 aacgcgcgct tggcaaggcg cccatgcccc acctcgcgac gcttcggagt gcctacgcgg
     7321 ccggtttcac cggtcgaata aggagggaaa ttgtagtgga gcatgaaccg ctcggaatat
     7381 tcgccctgga gggcatcgat gatttgctca tcgcgggcgg tccctaaagt agctacggcc
     7441 agcgcctggg tttccccgcg ggtaaagagc gcggagccat gcgcgcgggg aagtacgcca
     7501 gcacgaatgg tgatcggcct gactgtgcgt gtgtcgcggc catcgatgcg cggttcgcca
     7561 ttaaggatct gtgtgcgtac gatacgggct tcaatatcgc tgaagattga cttaaccagg
     7621 ttgacctgag cggtatccga gttgtccggt gctgcgtccg tcaatacctt gccgcgaacg
     7681 gcatcgatct tttcagtgcg cgcctgtttt tgccgaagac tgtaagcctg gcgcaattca
     7741 gcttcggcta ttgcggcgat ccgttcaacc aatgcagatt ctttttgcgg gggcggcaat
     7801 tcccagagcg gcttgccgac ctcgtcggcc agttcgttga tggcgttgat caccagctgc
     7861 atttgctggt gaccgaagac cacggcaccc agcatcacct cttcaggcag ttccagcgcc
     7921 tcggattcga ccatcaatac cgcctggctg gtacctgcta caaccagatt gagctgcgaa
     7981 gttttcagtt cggttgcagt cggattgaga acatattcgc cgttcaaata tcctacacgc
     8041 gccgccccga tgggaccatc gaaaggaatg ccggatatgg ccaacgccgc cgacgctcca
     8101 atcatggctg gaatatcaga atctacttca ttgttggaag atacaacggt cgcgatgacc
     8161 tgaatttcgt tgtaaaaacc ttcggggaac agcggacgaa tcgggcgatc aatcaagcgg
     8221 gacgttagga cttctttttc cgacggacgg ccttctcgtt tgaagaatcc gcccgggatc
     8281 ttgcctgcgg catacgttct ttcctgataa tccaccgtca gagggaagaa atcctgaccc
     8341 ggcttgacat cctttctgcc aacgacggtt accagtacca cggtatcgtc catgttcacc
     8401 actacggagc cgtgcgcctg ccgcgcgatt tctccggtct cgagcgtcaa agaatggcgc
     8461 ccgtagttca tggtttttct ggtgacgttc aagagaattc cttttgcatg ccgcaacgcc
     8521 ggggagcggt tcgctccggc cagcaagtgt ttcgatatga tggagccgac ttcattgtct
     8581 gctccttaaa ttactcggac tgaaaccgtc cgggacatgc gccgagattt tcgactactt
     8641 gcgcagtccg aggcggtcca gcaaggtgcg gtaacgatcc cggtccgagc gtcgcaggta
     8701 atccagcagt ttgcggcggc ggctcaccat tctcaacagc ccacggcgag aatgatggtc
     8761 cttcacgtga tctttgaaat gacctgttag atcgttaatt cgcgcagtaa gtaacgcgac
     8821 ttgtacttcc ggggaaccgg tgtcggcctt tgcccgttga tggtcctgca gaatctgcgc
     8881 cttttggctc gtcgaaatcg acatcaccca gtctccgaaa gaaagggcgg tattttacct
     8941 tcggactccc ggagtatcca agtattttag gggcttaatc gagcgccgcc ggcttttcgg
     9001 agggttttcg cacctgtgat accaggcgct tcgcggccag caatccgtcg ttagtgcttt
     9061 cgccgatccc gatgaagttt cccgccaaat catagagtct gaagaccccc ggaccaccac
     9121 cggcgctcgg gacgccaagg ccattaatga tgcgccgggt cgtttccgcc gggagatgca
     9181 aagcaggaag aaattcaacc agccggtcta ccggcaatag ccgggcacgg cgggaagatt
     9241 cattcatctc ggtcaagctt tccagatcta tggcattggt cacagtcaga tcgcggattc
     9301 ctgtgcggcg cagagaccct agataaccgc cacaccccaa tgccttgcca atgtcttcgg
     9361 ccagtacacg gatgtaggtt cccttgctgc agtctaccgt tatggtgatc gtatggcttg
     9421 taacgtcaat agcttgcagt cgctttataa taatctttct aggttctcgg accaccgttt
     9481 tgccggcgcg agcgtacttg tagagcggcg tgccatgctt ttttagcgcg ctatgcatgg
     9541 gcggaacttg ttcggttacg ccgataaatg aggcaagcac attttccagt cggctggcag
     9601 taaagtttgg ttctgcgcaa gcctccagga ttccctcggc gtctcccgtc gaacttacaa
     9661 ggccaagccg tatggacgct tcatatgcct tgtccgcgtc aagtaacgcg tgggaaaact
     9721 tggtggcttc acccaaacaa ataggcaata agcccgtcgc cagagggtcc agtgttcccg
     9781 tatgtccagc cttggaccac gagaacaagc gacgcacgcg ttgcagagca acgttagatg
     9841 acaggccggc gggtttgtcg agcaatagca cgccgtgatc catggccttt cagattggtc
     9901 ccgacaggat tgtcatggct tgccttcttc gccggggtgc gatgcgattg catcgtcgat
     9961 caatgtagaa atccgtacgc cccgctccac tgattcgtca tagatgaagc gaagctccgg
    10021 aactattcga agttttagtc ctttagcgag ctgcgatcgc aagaatccgg aagcatgctc
    10081 taatccgcct atggtcctgg cgacgctttc cggaccgccg agagtcgtaa aaaatacctt
    10141 ggcgtagtga taatcgctgc tgatctccac tccagtgagg gtcagcatga cgactctcgg
    10201 atccttgact tgcgtgcgaa tcagctcggc aagatctttc tgaatctggt ctgcgatacg
    10261 ggcaagcctg gaaggatttt ttgccatctg ctgaaccgtt cagaagtgaa agcttgacgc
    10321 ggcatgattt tcgatgccgc cgctgacatg caatcggcgt tacaggctgc gggctacttc
    10381 gaccacttcg taagcttcca cttgatcgcc aaccttcaaa tcatcgaagt tctttacgga
    10441 aagaccacac tcgaatcccg ctttaacttc ccgcacgtcg tccttgaatc gtttcaatga
    10501 atcgagctct ccggtgtgcg ccacgacatt gtccctgagc acgcggatga gggaaccgcg
    10561 tcggatcaag ccttcaagaa catagcaccc tgccaccgtc ccaaccttcg aaatcttgaa
    10621 cacttgacga atctctacca gaccgagaat gttttccttc ttttcggggg caagcatgcc
    10681 cgaaagcgcg gatttcactt catccacggc ttcataaatg atgttgtaat agcgaaggtc
    10741 gatgccatgg gctgcaacca attttctggc cgtcgcatcc gccctggtgt tgaagccaat
    10801 gatgactgca ttggaagcca aagccagatt gacgtctgat tcagtaatcg cgccgactcc
    10861 cgcgtgcacg atgtttacct tgacctctgc agtggacaat ttttcgagcg actgggacaa
    10921 ggcctcgtag gaaccctgca cgtccgcctt gatgatcaag gacaatgctt tagcttgacc
    10981 agtcgtcatc tggtcgtaca cgccttcaag cttcgccgtt tgctgcttgg ccagcttgac
    11041 ctcacggaac ttcccttgac ggaacagcgc gatttcccgc gccttgcgtt catccccgag
    11101 agcgatcatt tcttcgcctg ccagaggaac gtctgacagc ccttgaatct caaccgggat
    11161 ggacggcccg ccttcctgta tctgatgacc ggcctcatcc agcatggcgc gcacgcgtcc
    11221 aaataccgcg ccggcgagca cgacgtcgcc acgctttagc gtgccggatt gaaccagaat
    11281 ggtcgctacc gggccgcgcc ctttatcgag ctttgattca atcacgacgc cgcgagcggg
    11341 agcatctttc ggcgccttca attccatgac ttccgcttgc aacaatatgc gctcaaggag
    11401 ttcgtcgatg ccttggccgg ttttcgcaga cacgtcctga aacatggcat cgccgcccca
    11461 ttcctccggt acaacttcgt gcgcggccag ttcctgcctg attttctctg gattagcttc
    11521 cggtttatcg atcttgttga ccgctacgag caccggtacc ttggcggctt tggcgtgatt
    11581 gactgcttcg agcgtctgcg gcattacgcc gtcatcggct gcaacaacca aaaccaccaa
    11641 atcggtaacc ttggctccgc gcgcacgcat cgctgtaaaa gcctcgtgac ctggcgtatc
    11701 cagaaacgtg atcatgccgc gtggcgtgtt cacgtggtag gcgccaatgt gttgcgtgat
    11761 gccgccggcc tctccgctgg ctacgcgagt gcgtcgaatg tagtcgagca gtgatgtctt
    11821 gccatggtcg acgtggccca tcaccgttac gactggggct cgcggttcag cgacgacatc
    11881 gtgtttttcg ccttcgtcag ccagaaatga ttcgggatcg tcgagcttcg ccggtttggc
    11941 aatgtgcccc atctcctgaa ccacaatcat cgccgtttcc tggtcgatca cctggttgat
    12001 ggtgaccatg ctgcccagct tcatcagcac cttgataact tccgctgctt tcacagacat
    12061 cttctgcgca agggccgcga ccgtaatggt ctcgggtacc aacacttcgc gaataacggg
    12121 atccggcgct gtaaaagttt gctctggagc gacggtttcg gtcttgtgcc ggcctcctcg
    12181 acccgagcgg gtatgccagc tctgcgctcc tccggtcacg tccccgcgtg tcttgagccc
    12241 ccttcttttt gccgattcat ccttccaggc atccggcttc ttcgctggtt ttggcttggc
    12301 cgccttttcg ctgggcttgg ccgctggctt gtgcagagta ccttcagtgg ttattttttt
    12361 tggctcttcc gcagccttgg cgactggtgc aacagcgact ggctctgctt gtacgaccgg
    12421 tggttcggct tccttggtag atttgcgctt gcgctgttgc ttctgcgcgg cttctgcggt
    12481 ttgccgggca atcaattctg cctgcttgcg cgcctcttcc tcccttagtg ccgtttgttc
    12541 ggcgttgaca ttgggtggcg tagccggtgt agcgggcgcc ggaggaggcg ccagatccac
    12601 atcatgaaca acgttctctg cggtttccga ggcggtatcg cgttttacga ggacgcgttt
    12661 cttttttacc tcgacctgga tcgtacgtgc cttgccggtg ctgtctgatt tcttgatctc
    12721 gctggtttgc cggcgggtca gggtaatctt gtttttcgtg tccttggcac cgtggacacg
    12781 gcgcagataa tccagcaact gggtcttatc gtgttcggtg agaactgtat cgtcggcaag
    12841 cgatttgccg atacccgcgg cctgcaattg ctcgaggagc atcgcggatg ccaatcccaa
    12901 ttcctttgca aactgagcaa cgttcatttg cgccataagt tgttacccct gacttgcagc
    12961 aaaccacggt gcgcgcgccg tcataatcaa ttgtttggcg cgctccgagt caatgccagt
    13021 caattcgatc aaatca
//
LOCUS       DATMZQ010000414         2913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_331434, whole genome shotgun sequence.
ACCESSION   DATMZQ010000414 DATMZQ010000000
VERSION     DATMZQ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331434"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /gene="rpsD"
                     /locus_tag="VN496_13145"
     CDS             complement(<1..360)
                     /gene="rpsD"
                     /locus_tag="VN496_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXF17947.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKGEKCFSDKCSIERRAYAPGQHG
                     QKNLRRSDYGTQLREKQKIRRIYGMLERQFRNTYKLAARAKEVTGEALLQMLESRLDT
                     VAYRMGFGSSRTEARQVV"
     gene            complement(378..776)
                     /gene="rpsK"
                     /locus_tag="VN496_13150"
     CDS             complement(378..776)
                     /gene="rpsK"
                     /locus_tag="VN496_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXF17948.1"
                     /translation="MAKATAPAARVRKKVKKNVAEGIAHIHASFNNTIVTITDRQGNA
                     LSWATSGAAGFKGSRKSTPFAAQIAAEQAGRAAQECGVKNLEVRIKGPGPGRESAVRA
                     LNSVGFKITSISDVTPVPHNGCRPPKKRRI"
     gene            complement(804..1193)
                     /gene="rpsM"
                     /locus_tag="VN496_13155"
     CDS             complement(804..1193)
                     /gene="rpsM"
                     /locus_tag="VN496_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXF17949.1"
                     /translation="MARIAGVNIPNHQHAVIALTSIYGIGRARSRAICAAAGIKGSIK
                     MKDLSDNDMEKLREQVAKFTVEGDLRREVTMSIKRLMDLGCYRGTRHRRGLPARGQRT
                     RTNARTRKGPRKAIQRSGGATPAPAAK"
     gene            complement(1237..1350)
                     /gene="rpmJ"
                     /locus_tag="VN496_13160"
     CDS             complement(1237..1350)
                     /gene="rpmJ"
                     /locus_tag="VN496_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXF17950.1"
                     /translation="MRVQASVKKMCRNCKIVRRNRVVRVICTDPRHKQRQG"
     gene            complement(1396..1614)
                     /gene="infA"
                     /locus_tag="VN496_13165"
     CDS             complement(1396..1614)
                     /gene="infA"
                     /locus_tag="VN496_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXF17951.1"
                     /translation="MAKEETIQMQGEILETLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVDLTPYDLTRARIVFRAK"
     gene            complement(1624..>2913)
                     /gene="secY"
                     /locus_tag="VN496_13170"
     CDS             complement(1624..>2913)
                     /gene="secY"
                     /locus_tag="VN496_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXF17952.1"
                     /translation="KFGDLKRRLLFLLGALIVYRIGAHVPVPGIDPVQLAELFKAQKG
                     GILDMFNMFSGGALSRFTIFALGIMPYISASIIMQLLTVVSPTLEALKKEGEAGRRKI
                     TQYTRYGTLFLATFQGLGIAIALESQRGLVIDPGLAFRFTTVVTLVTGTMFLMWLGEQ
                     ITERGIGNGISLIIFAGIAAGFPRAIGGTLELARTGAFSIPLVLLLLVGAILVTGFVV
                     FVERGQRKILVNYAKRQVGRKVYGGQSSHLPLKLNMSGVIPPIFASSIILFPATLAGW
                     FGSNESMTWLRDISGTLHPGQPIYVLLYASAIIFFCFFYTALVFNPKETAENLKKGGA
                     FVPGIRPGDQTAKYIEKITMRLTLAGAIYVTLVCLLPEFLIVKWNVPFYFGGTSLLII
                     VVVTMDFMAQVQAYVMSHQYESLLKKANFKGGMFPTR"
BASE COUNT          559 a          909 c          813 g          632 t
ORIGIN      
        1 cacgacctgg cgcgcctccg tgcgtgatga gccgaaaccc atgcgatagg cgacggtgtc
       61 cagacggctc tccagcatct gcaggagcgc ctcgccggtg acttccttgg cgcgcgcggc
      121 cagcttgtag gtgttgcgga attggcgctc gagcattccg taaatacggc ggatcttctg
      181 cttttcccgc agttgcgtgc cgtagtcgga acggcgcagg tttttctgtc cgtgctggcc
      241 aggagcatac gcgcggcgct cgatcgagca cttgtcgctg aagcatttct cgcctttcag
      301 gaacagcttt tcgccttcgc ggcggcactg tcggcacttc gggtctagat tccttgccac
      361 agtttatctc cttgggatca gatacggcgc ttcttgggcg gacggcagcc gttgtgcgga
      421 accggcgtga catccgagat gctggtgatc ttgaacccga ccgaattcaa tgcacgaacc
      481 gcggattcgc ggcccgggcc ggggcccttg atgcgcactt ccagattctt tacgccgcac
      541 tcctgtgcgg cacgacccgc ttgttctgcc gcaatctggg ctgcgaacgg ggtggacttg
      601 cgtgaaccct tgaaacccgc agccccggag gttgcccagg acagcgcatt tccctgacga
      661 tcggtgatcg tgacaatggt gttgttgaag gacgcgtgaa tgtgggcgat gccttctgcc
      721 acgttcttct tgaccttctt gcggaccctt gctgccgggg cggttgcttt tgccatgttg
      781 gagaatccct gagctctgtt cgtttacttc gctgctggtg ccggagtcgc gccgccgctg
      841 cgctgaatcg ccttgcgcgg tcccttgcgt gtgcgcgcgt tggtgcgggt acgctgaccg
      901 cgcgccggca aaccgcggcg atgccttgtg ccgcggtagc agcccaggtc catcaaccgc
      961 tttatgctca tcgtaacctc gcggcgcagg tctccctcga cggtgaactt cgcaacctgc
     1021 tcgcgcagct tctccatgtc gttgtccgac agatccttca tcttgatcga acccttgatg
     1081 ccggcggcgg cgcaaatggc gcgcgagcgg gcgcggccga tgccgtagat cgaggtcagc
     1141 gcgattaccg catgctgatg gttgggaatg tttacaccgg cgatacgagc catatgtcgt
     1201 cctgcgtcct gtaagttata ggtgcgtttg cgccggttaa ccctggcgtt gcttgtgccg
     1261 cggatccgtg cagatcacgc gcacaacgcg attgcgccgg acaatcttgc agttccggca
     1321 catcttcttg accgatgctt gtactctcat tttctgcttc ctctgtctgc cattcgtggc
     1381 gccctgtgcc cggccctact tcgcgcggaa aacgatacgc gcgcgtgtca aatcgtaagg
     1441 cgtcaaatcc accgtgactt tgtcgcccgg caagatccgg atgtagtgca tccgcatttt
     1501 tccggaaatg tgtcccagta ccacatggcc gttctccagc ttcacgcgaa acgtcgcatt
     1561 aggcagggtt tccagaattt ctccctgcat ctgaatggtt tcttccttgg ccatgctcct
     1621 ccgctaccga gtcgggaaca tcccgccctt gaagtttgcc ttcttgagca ggctctcgta
     1681 ctggtgcgac ataacgtacg cctgcacctg agccatgaaa tccatggtca cgaccacaat
     1741 gatcagcaga ctcgtaccgc cgaaatagaa cggtacattc catttcacga tcaaaaactc
     1801 cggcaacagg cacaccagcg tgacgtaaat tgcaccggcc agggtcagcc gcatggtgat
     1861 tttctcgatg tactttgcgg tctgatcacc ggggcggatt cccggaacaa acgcaccgcc
     1921 tttcttcagg ttctctgcgg tttccttggg attgaacacc agggccgtgt agaagaagca
     1981 aaagaaaata atcgccgatg catacaacaa tacgtaaatc ggctggccgg gatgcaacgt
     2041 gccactgatg tcgcgcagcc aggtcatgct ctcgttgctg ccaaaccagc cggccagcgt
     2101 ggccgggaac aagataatgc tggaggcaaa tatcggcgga atcacgcccg acatattcag
     2161 cttcaacggc agatgcgaac tctgcccgcc atatactttt cttccaacct gccgcttcgc
     2221 gtagttcacc agtatcttgc gctggccacg ctccacgaac accacgaaac cggtcaccag
     2281 tatcgcgccc accaacagga gcagaaccag aggaattgaa aatgcccccg tgcgcgccag
     2341 ctccagcgtg ccgccgatcg cccgcggaaa gcctgccgca atcccggcga aaatgatcag
     2401 cgaaatcccg ttgcctatgc cccgctcggt gatctgctca cccagccaca tcaggaacat
     2461 tgttcccgtt accagtgtca ccaccgtcgt gaaacggaac gcgagtcccg gatcgatcac
     2521 cagcccgcgc tgcgattcca gcgcgatcgc gattcccaac ccctggaaag tcgccaggaa
     2581 cagcgtgccg tagcgcgtgt actgcgtaat cttgcggcgg cctgcctcgc cttccttttt
     2641 cagcgcttcg agcgtcggcg aaaccacggt cagcagctgc atgataatcg aggcggaaat
     2701 ataaggcatg atccccagcg cgaagatggt gaatcgcgac agcgcaccac ccgagaacat
     2761 gttgaacatg tccaggatgc cgcccttctg cgccttgaac agttctgcga gctgtaccgg
     2821 gtcgatcccg ggcaccggaa cgtgcgcgcc gatgcggtac acgatcaacg cgcccagcag
     2881 aaacagcaaa cggcgcttga gatcgccgaa ttt
//
LOCUS       DATMZQ010000415         4806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2748227, whole genome shotgun sequence.
ACCESSION   DATMZQ010000415 DATMZQ010000000
VERSION     DATMZQ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748227"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VN496_13175"
     CDS             complement(<1..625)
                     /locus_tag="VN496_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXF17953.1"
                     /translation="MFFLIRVLLRLLLRVRVLGDLAPFAHADRLLIVANHHSRLDAAI
                     LALFLPRDPVVIVPPDEAGTAFRRWLLVRVRHEILDLNHPLNMKRVLRLLRSGIPVVL
                     FPEGRIVRNGAVMKVYPVPALIALKSGASVLPVHIETAGQQFSRSGPGSLLGRWLPRI
                     TLRVFSATRIDGGAAGTARRRRAHATRRLAAVMQTMAVQSHACKPIFE"
     gene            931..1875
                     /locus_tag="VN496_13180"
     CDS             931..1875
                     /locus_tag="VN496_13180"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXF17954.1"
                     /translation="MDMIVPRELLLPAPRSTNSPETEYFFSEQLSALYHAEQHAMWLR
                     WNPGPRPNFNTGLLQDLDRYCQFVTHSGGAVQHKGERMPLEYAVLASKVPGVFNLGGD
                     LCLFARLIDAQDRQGLLEYGRACVKVLYRNYQGHGLPITTISLVQGECLGGGFEAALS
                     SDLIIAERQARFGFPEILFNLFPGMGAYSFLERKVGKQATERLISTGKLYSADDMLAQ
                     GVIDLVVDQGCGETEVANLIKSRGRNRKALAGIAAARRCVHKLDYEELLGVVEVWVDT
                     ALSLTGRDLKLMQRLVSRQNELRPSDHQHDIIMARQLH"
     gene            1937..3907
                     /locus_tag="VN496_13185"
     CDS             1937..3907
                     /locus_tag="VN496_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF17955.1"
                     /translation="MAQDLSEYTIHEAAARLGIPLQKLRRWDGQGVLVARRTDGGHRR
                     YAREIIDGLAGSAGVTPNDKADSAKPETGRMEDQLAAARRTLKEKRRIIQLLLESESR
                     YRDLVETSHDLIWTTDALGRFTYLNNACVDLFGVRPKDLLGGCFFEYEARPQHVSNRR
                     FLSALRKSGEVKNYLTHLISTDGSDRWVGINARVTHDEEGRIVGLRGTARNVTEQHRA
                     ALEIERLATHDALTGLPNRVSLQKALEAALADGEKGSVALLDVDHFKYINDNFGHRTG
                     DQLLIAIGGLLKDLVKEDEGVQVFRLGGDEFAMHLPNALRSDAAKVGERMLSALRRYK
                     FPVPGQGCLSTLTGSVGVATYPFHGADVATLLANVDIAMYQAKDNGRNRVRLHDQNPD
                     DLRSTHKRVHWARELREILDDNRVVLYSQPVIRLADRTPVHHEVLVRVVDRSGGIVLP
                     GEFIEVAESLGMAQEIDLRVVSKVIEVLKGCRGQKTRYFVNLSRTSISDPQWVRRFHN
                     MLAAAPIDHSQLVFEITETAAMSSVDVTQEFIAQMKHLNCRFALDDFGAGFSSFYFLK
                     RFEVDYLKIDGSFVRELASDSASRLFVRALCDVARGLNKQVIAEWVEDTAVMAILVEM
                     GVQYGQGFLFARPMPFPATVSEERQQRKIKRA"
     gene            complement(3926..4474)
                     /locus_tag="VN496_13190"
     CDS             complement(3926..4474)
                     /locus_tag="VN496_13190"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXF17956.1"
                     /translation="MNSDFTEKKITSRAAYRGKLLKVNEDKVSLPDGGTALREYVLHP
                     GAAIILPLFDDGTVLLERQFRYPLGRHFYELPAGKLEPNEPPIETAKRELLEETGYQA
                     AEWRELGRFHPCVGYSDEQIDFFLARKLEFKSTKLDEGEFLETLRVPLADTIDWIRRG
                     RISDTKTILGLFWAEKLINENW"
     gene            complement(4471..>4806)
                     /locus_tag="VN496_13195"
     CDS             complement(4471..>4806)
                     /locus_tag="VN496_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXF17957.1"
                     /translation="YAFVMLLLMFAMTGIPPTVGFWAKLFVLQAALATGYVWLVVTAV
                     LFSVIGAFYYLRVVKLMYFDAPTDTHAIEPYADVRAVMSVNGLAIIVLGLTPGALLAL
                     CQRAIQLSL"
BASE COUNT         1100 a         1327 c         1349 g         1030 t
ORIGIN      
        1 attcgaatat cggtttgcag gcatgcgact gcacggccat tgtttgcata actgccgcaa
       61 gccgacgcgt tgcatgggcc cgccggcgtc gcgcagtgcc ggccgcgcca ccatcaatcc
      121 gggtcgcgga aaagacccgc aacgttattc gcgggagcca acgacctagg agcgatccgg
      181 gaccggatct gctgaactgt tgccctgctg tttctatgtg caccggcagc acgcttgcgc
      241 cactcttcaa tgcaatcaga gccggtaccg ggtagacctt catgacagcg ccgttccgaa
      301 caatgcggcc ttccggaaaa agtaccactg gaattccgct tcgcagcaga cgaagcacgc
      361 gcttcatgtt caacggatgg ttcagatcca ggatctcatg gcggactctg accagcaacc
      421 agcggcggaa cgcggttccg gcttcatcgg gtggcacgat aaccacggga tcgcgcggca
      481 ggaacaacgc aagtatggcg gcgtccaggc gagagtgatg attcgcgacg atcagcagcc
      541 ggtcggcatg ggcaaacggt gccagatccc ccaatacacg aacgcgcaga agcaaacgaa
      601 gcaacacgcg tatcaaaaaa aacattcgca ctccaaattc tcgaaaatga aaaacgaaat
      661 ccgatgtttg tgtcttatta ttgaatcagt tacgaacatg tcgtcccgtt cgaaaatcgg
      721 caaatttcac gctgtatttg tcgccttaga atcggtattt aatccaacac attgactaga
      781 cagctaaaaa gttaaaccta tcagagcgtt agatttgctt tttacatgcg tttggggcta
      841 aagtccacac tggaatactg ccttttccgg gggaaacacg aagcggcggt atctgcatca
      901 cctcatacca tttgcaacag gagaattaaa atggacatga ttgtcccgcg ggaactattg
      961 cttcctgccc cacgctctac taactcccct gaaaccgaat atttcttctc cgagcaattg
     1021 tctgcgcttt atcacgccga gcagcatgcg atgtggcttc gatggaaccc cgggccgcga
     1081 cccaacttta ataccggtct tcttcaagac cttgaccgat attgccaatt cgtgacccat
     1141 agcggaggag ccgtccagca caagggcgaa cgcatgccac tggagtatgc ggtactagcg
     1201 tcaaaagtgc ctggcgtctt caacctggga ggagatctgt gcctgttcgc gcgcctgatc
     1261 gatgcacagg atcgacaagg gctactcgaa tacggccgcg cctgcgtaaa agtgctctac
     1321 cggaattatc agggacacgg cttacccatt acgaccattt ccttggttca gggcgaatgt
     1381 ctcggaggag gattcgaagc cgccctttct tcagacctga ttatcgccga gcgacaggcg
     1441 cgctttggct ttccggaaat cctgttcaac ctgtttccgg ggatgggcgc ctactctttc
     1501 ctggagcgca aggttggcaa gcaggcgacc gaacggctga tatcgactgg caaactgtat
     1561 agtgccgatg atatgctggc gcagggagtg atagatctgg tcgtggacca gggttgcggc
     1621 gagactgaag tagccaattt gattaagtcg cggggtcgca atcgtaaggc gttggcgggt
     1681 attgcggctg caaggcgttg cgtccacaag ttggactacg aggaattgct tggtgtagtg
     1741 gaagtttggg tcgataccgc gcttagcctg actggccggg atctcaagct tatgcaacgc
     1801 ctggtgtccc gtcagaacga gttgcgccca agcgaccacc agcacgacat catcatggca
     1861 cgacagttac actgatttct ccggtgcggg tcatgcgccg gcccgatcaa ctgggagaac
     1921 gcggcagcag gcgtatatgg cgcaagatct ttccgagtat acgattcatg aagcggctgc
     1981 acgcttgggc atcccgctgc agaaattgcg gcgatgggat ggacaagggg tactggtagc
     2041 ccgccggaca gacggaggcc accggcgcta tgcgcgcgag atcatagacg gactcgccgg
     2101 ctcggcgggc gtaacgccta acgacaaggc cgattctgcc aagcccgaaa cgggacggat
     2161 ggaagatcag ttagccgccg cccgccgtac attgaaggag aagcggcgga tcatccagtt
     2221 gttgctggag agcgagtccc gctaccggga tctggtcgaa acttctcacg atctgatttg
     2281 gaccacagac gcgctgggcc gcttcacata cctgaacaac gcctgcgtcg atttgttcgg
     2341 tgtgcgacca aaggacttgc tgggtggctg cttctttgaa tatgaagccc gaccccagca
     2401 cgtgtcgaat cgccggtttc tttcagcgtt gcgcaaaagc ggcgaagtaa agaattatct
     2461 cacccacctg atttctacag atggttcgga tcgctgggtc ggcatcaacg cccgcgttac
     2521 gcacgacgaa gagggacgga ttgtcgggtt gcgtggtacc gcgcgcaatg tcaccgaaca
     2581 acatcgcgca gcattggaaa ttgaacgttt ggcgactcat gatgcgctga ccgggttgcc
     2641 caaccgggtt tccctgcaga aagcgctgga ggcggctttg gccgacggcg aaaaaggctc
     2701 ggtcgcactg ctcgacgtgg atcatttcaa atatatcaat gacaatttcg ggcaccgcac
     2761 cggggatcag ttgttgattg cgatcggagg gttgctaaag gacctggtaa aagaagacga
     2821 aggcgtccag gtgttccggt tgggcggaga cgaattcgcg atgcacctgc ccaatgcgtt
     2881 acgcagcgac gccgccaagg tcggtgagcg catgctatcg gccctgcgcc gctacaaatt
     2941 tcccgtgcct ggccaaggct gtctttcaac gcttactggt tcagtcggcg tggccaccta
     3001 ccctttccat ggcgctgacg tggcgacgct tcttgcgaac gttgacatcg cgatgtatca
     3061 ggcaaaggac aacggtcgca accgtgtccg gctacacgac caaaatccgg atgatttgcg
     3121 cagcactcac aagcgcgtgc actgggcacg cgagttacgc gagatcctgg acgacaatcg
     3181 ggtcgtgctc tattcgcagc cggtgatccg gctcgcagat cgaacgcccg tacaccatga
     3241 ggttctggtg cgcgtagtgg accgcagtgg cggcattgtg ctgcccggag aattcatcga
     3301 agtcgccgaa tcgctcggaa tggcgcagga aatcgatctg cgcgtagtga gcaaagttat
     3361 tgaagtattg aagggctgtc gcggccaaaa aacgcgctat tttgttaatt tgtcccgcac
     3421 cagcatttcc gatccacagt gggtgcggcg cttccacaat atgctggctg ctgcgcccat
     3481 agaccactcc cagctggtat ttgagatcac tgaaacggcg gccatgtcga gtgtcgatgt
     3541 gacccaggaa ttcatcgccc aaatgaagca tctgaactgc cgttttgcgc tggacgattt
     3601 cggtgccggc ttcagttctt tttattttct caagcgtttc gaagtggatt acctgaagat
     3661 cgatggcagt tttgtgcgcg aactcgcttc agacagtgcc agccggctgt ttgtgcgagc
     3721 cctttgcgac gtggcgcgcg ggctgaataa acaagtcatc gcagagtggg tagaggatac
     3781 agctgtcatg gctatcctcg tggaaatggg cgttcaatat ggccagggct tcttgtttgc
     3841 ccgcccgatg ccatttccag ctacagtttc ggaagagcgt caacaacgca aaatcaagcg
     3901 cgcctgaatt ccgctgcgct gcagtctacc aattctcatt gattagtttc tcggcccaga
     3961 acaagccgag aatggtcttg gtatcactga tgcggccgcg acgaatccag tcgatcgtgt
     4021 cggccagtgg cacgcggagc gtttctaaaa attctccttc gtccagtttt gtgctcttga
     4081 attcaagctt gcgcgccaga aaaaaatcta tctgttcgtc cgagtaacca acgcaggggt
     4141 gaaaccgccc caattcccgc cattcggctg cttgataccc ggtctcctcc agcagttccc
     4201 gcttggccgt ttcaatgggc ggctcattcg gctctaactt gcctgccggc aactcataga
     4261 aatgcctgcc cagcggatac cgaaattgac gctccaacag caccgtacca tcatcgaata
     4321 acggcaggat gattgctgcg cccggatgca agacatattc gcgtaaggcg gtaccgccat
     4381 ccggcaggct taccttgtcc tcatttactt tgagcagctt gccgcgatag gccgcccgcg
     4441 aggtaatttt cttttcagtg aaatctgaat tcacagggat agctggatag cgcgctggca
     4501 tagggcgagt aaggcgccgg gcgtaagtcc cagcacaatg atcgcaagcc cgttgaccga
     4561 catgacggca cggacatcgg catacggttc aatcgcatgc gtgtcggtag gcgcgtcgaa
     4621 atacatcagc ttgaccacgc gcaaataata gaacgcccca atgaccgaaa aaaggaccgc
     4681 agtcaccacc agccacacgt atcccgtggc cagtgccgct tgcagcacga acaatttcgc
     4741 ccagaagccc accgtaggcg gtatgccagt catcgcaaac atgagcaaca gcatcacgaa
     4801 ggcgta
//
LOCUS       DATMZQ010000416         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1549590, whole genome shotgun sequence.
ACCESSION   DATMZQ010000416 DATMZQ010000000
VERSION     DATMZQ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549590"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1053
                     /locus_tag="VN496_13200"
     CDS             274..1053
                     /locus_tag="VN496_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HXF17958.1"
                     /translation="MEPAISPLLFAALLFVGMLGLLEIGRRVGIRRKPKESEGERASL
                     GTIEGAMFALFGLVVAFTFSGAASRFNEKRTLIAEEANTIETAYLRLQLLSQEARPEL
                     QELFRGYVDSRLQTYRKLPNMEAAKIEMARSKKFQEEIWTKAIAATRLPNSHPDVGKL
                     LLPALNDMIDITTIRAMALQTHPPNIIYALLFSLGLICSLLAGYRMAIGQHRSWLHIL
                     SFAVITVIIVYVIIDVEYPRAGLIRLQAFDQLLVNVREGMK"
     gene            complement(1498..>2927)
                     /locus_tag="VN496_13205"
     CDS             complement(1498..>2927)
                     /locus_tag="VN496_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17959.1"
                     /translation="NEIFYEGGRYWRTLALARLSMLPFFAFGVAIVFFWGRRIDGNVA
                     GLLGVFFFTSLPAILAFAGFAYTDMPVGVLIAGSAFMFVRWLDLPSMQNSILFGVLTA
                     LALLSKFTALLFLPACWVAIVLCWIGTNQRREWRLYFRQAVWIVLTFFVIFWGGYRFS
                     VAPLKTVFLNPGQDIHALHVPQAVKRLLYFAVDKPIPAPAFFKGVTASIRSGAVLGRP
                     SYLLGEIRQTGWWYFFIVAIGVKTPLAFLLLGVLGLFLSLMRGLKERNWQLAAPGACI
                     VAILVATFPVRVNYGVRHILCIYPFLSVMAAYGAAKIYEQRERWGLQGRVALAGLLAW
                     HLLVTSHVHPDYVTYFNELAGRQPQKILMWGCDYDCGQDVARLARLLGERKIQHVTLG
                     VFGNNDFKRSGLPPFESLDPYQRPKGWVAASVRIVETGDAFLGGSHRDAFQWLSACKP
                     LARAGETIYLYEVPCGSSDSVVSNP"
BASE COUNT          767 a          787 c          760 g          613 t
ORIGIN      
        1 ttctacctca tagaacactg cgccgtatcg aagccaccgt gccaaaatga gaggcagcct
       61 gcttccgaaa catcgattaa cgcgaccacc gggattttac atcttcaagt cgattggcga
      121 taaatcacca cttcaccaaa ctagcttggc ccggacgccg ttttccaggt agttggtgta
      181 agcgccagtt aacacccttt gggaccggat gaacacgccc gatccacaag aatcgttatc
      241 cttgggtaaa attgggccgg atcggagagt cagatggaac ctgctattag ccctttactt
      301 tttgcggcac tgctcttcgt cggtatgctt ggcctgctgg aaatcggccg tcgtgttggc
      361 atcaggcgca agccgaaaga gtcggaaggc gagcgggcca gtctgggtac catcgagggc
      421 gcgatgtttg ccctgtttgg attggtggtg gcgtttacgt tctcgggtgc ggcatcgcgt
      481 ttcaatgaga aacggacgct aatcgccgag gaagctaaca ccatcgaaac cgcatatctc
      541 cggttgcagt tattgtcgca agaagcgcgg cccgaactgc aagagttgtt tcgaggatat
      601 gttgactctc gattgcaaac ctatcgcaag ctgccaaaca tggaggccgc taagatcgag
      661 atggctcgat ccaaaaagtt ccaggaagag atttggacaa aagcaattgc cgctacacgg
      721 cttccgaact cccatcctga cgtaggtaag ctcctgctgc ccgcgctaaa cgacatgatc
      781 gacattacta cgattcgtgc gatggcgctg caaacccacc cgccaaacat catctatgca
      841 ctgttgttca gcctcggtct tatttgctcc ctgctggctg ggtatcgaat ggcaattggc
      901 cagcaccgga gttggctcca tatcctgagt ttcgcggtca tcacggtcat catcgtatat
      961 gtgatcattg atgttgaata cccacgggcc ggtttgatcc gcctccaagc gtttgatcaa
     1021 ctgttggtca atgttcgcga aggcatgaag tgaagagaac gggatgcgag ctaaaccatg
     1081 gctcgtaaaa agttagcgtc gatcggtgaa acatcgccat ttcaccaact acttaggctc
     1141 gccgccagat agttcgtgta atcgactgtt aacaacaatt gaacgccaaa agtcccgttc
     1201 gggtaggagc atagtattca tactgttgta gtgatgcttc gccgagccgt attaaaggcc
     1261 cttctggcaa agtaagcgcc aaaaaccagc agtcacacaa caaagccaaa cttcattgag
     1321 ctggatgatt gcagtggata ttcggcgaga atgctccgga agactggacg aaaaaaatca
     1381 gcgctggcaa gcccaagggg ttagcaactg aagagatgcg gggatcgatt tcatgtgtat
     1441 ccggaaagat aggcccaacg atttcgagac cgttactttg cctttccggc aactcaatca
     1501 gggattactt actacactgt cgctggagcc acaagggacc tcgtagagat agatcgtctc
     1561 tcccgcacgt gccaacggtt tacaggccga cagccattga aaagcgtcac gatgagagcc
     1621 acctaggaac gcgtctccag tctcaactat tcgcacgctt gctgcgaccc atcctttggg
     1681 tcgctgatag ggatccaggc tctcgaatgg tggaaggcca gaccgtttga aatcattatt
     1741 tccgaagacg ccgagtgtta catgctgaat cttgcgctcg ccgagcagtc gcgccagtcg
     1801 cgccacatcc tgaccgcaat cgtagtcaca accccacatc aatatttttt gtggttgcct
     1861 cccggccaat tcgttgaagt aagtcacgta gtccggatgt acatgggaag ttaccagtag
     1921 gtgccacgct agcagtccgg ccagcgctac gcggccttgc aagccccatc tctcgcgctg
     1981 ctcgtagatt ttcgccgcac cgtatgctgc catcacggac agaaagggat agatgcagag
     2041 tatgtgtctg accccgtagt tcactctcac gggaaaggtc gcaacaagaa tcgcgactat
     2101 gcacgcaccc ggcgcggcta gttgccaatt gcgttctttt aaaccgcgca tcaggcttag
     2161 gaataagcca agaactccca acagaaggaa tgccagcggc gtcttaacac cgatcgctac
     2221 gatgaagaag taccaccatc ccgtctgacg aatctcgcct aggaggtacg atgggcgccc
     2281 taacacggca ccgctgcgta tactcgcagt tactccctta aaaaacgctg gggctggtat
     2341 gggcttatca accgcaaagt aaaggaggcg cttgacggcc tggggcacgt ggagggcgtg
     2401 gatgtcctgc ccggggttta ggaaaacagt cttcagagga gcgacggaaa acctgtaccc
     2461 gccccaaaag atcacgaaga atgtaaggac tatccagacc gcctgccgga aatagagacg
     2521 ccattcgcga cgctgattag tacctatcca acaaaggaca atcgcgaccc aacaagcggg
     2581 caaaaacagc agtgcggtga atttcgataa caaagcgagt gcagtgagaa ccccgaaaag
     2641 tatcgagttc tgcattgacg gaagatccag ccaacgcacg aacatgaacg cgctccccgc
     2701 aatgagaacg ccaaccggca tatcggtata tgcaaagcct gcaaaggcca aaatagccgg
     2761 caagctggta aagaagaaga caccgagcaa acctgcaacg ttgccgtcga tcctgcgacc
     2821 ccaaaagaaa acaatggcga cgccgaatgc aaagaatgga agcatgctga ggcgagccaa
     2881 cgcaagggtg cgccaatacc tgcccccctc atagaaaatc tcattgc
//
LOCUS       DATMZQ010000417         4026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2062351, whole genome shotgun sequence.
ACCESSION   DATMZQ010000417 DATMZQ010000000
VERSION     DATMZQ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062351"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VN496_13210"
     CDS             complement(<1..273)
                     /locus_tag="VN496_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HXF17960.1"
                     /translation="MKRVLLIKLTSMGDLIHALPALSDATLAHPQIEFDWAIDENFQE
                     IARWHPAVKDVIPTNHRAWREAIMHPSTMGSVSDVISRLKAKQYDLV"
     gene            complement(251..913)
                     /locus_tag="VN496_13215"
     CDS             complement(251..913)
                     /locus_tag="VN496_13215"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXF17961.1"
                     /translation="MAPPHSWKVINADALKKAAVIAEPYPHLIIDNFIVPEALADVVK
                     SFPVVPKRGSVPLDAVSSSGTFAALMEEMHSTELRELIGERLGMDLSDKPAMITLRGH
                     TDTKDGDIHTDSKSKLVTLLLYLNPGWTASGGRLRLLYNNRDLTPYAAEISPEAGHCV
                     IFKVTPDCWHGHEIYIGERRSVQLNYVTSDEARRGHLSRHRFSAFLKRLFSRKNEESP
                     VN"
     gene            1108..1617
                     /locus_tag="VN496_13220"
     CDS             1108..1617
                     /locus_tag="VN496_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXF17962.1"
                     /translation="MDVGKKFTLFAIVLALTALAAGIYAGWSRPIAPADINFIGVKGD
                     RFHMSSFKGKVVLVNFWATSCTVCIGEMPKMVQTYQKYRDQGLETVAVAMSYDPPDRV
                     FAYAQQNKLPFHVTLDIFGTAMHAFGGIRGTPTTFLVDRRGNIVKRFEGEPDFAELGE
                     LIEKELLKA"
     gene            complement(1659..2633)
                     /gene="lipA"
                     /locus_tag="VN496_13225"
     CDS             complement(1659..2633)
                     /gene="lipA"
                     /locus_tag="VN496_13225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXF17963.1"
                     /translation="MAGKTWRSTSVTTPTKSERQTGEQKTGRNPVKIVPIIPLKKPDW
                     IRVKAPGSPRFQEIKQALREHNLHTVCEEASCPNIGECFGKGTATFMILGDLCTRRCP
                     FCDVAHGRPLAPDSQEPGNLARTIAAMKLKYVVITSVDRDDLRDGGAQHFAECIRAVR
                     KMSSQTRIEILVPDFRGRMTIALDRLFAHPPDVMNHNLETVPRLYKQARPGADYLHSL
                     KLLRDFKQVCPDIPTKSGLMLGLGETDAEILQVMRDLREHDVEMLTLGQYLQPTRGHL
                     PVLRYVEPARFEQFEQEALAMGFRHAACGPLVRSSYHADQQAHEAGVS"
     gene            complement(2600..3196)
                     /gene="lipB"
                     /locus_tag="VN496_13230"
     CDS             complement(2600..3196)
                     /gene="lipB"
                     /locus_tag="VN496_13230"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXF17964.1"
                     /translation="MRRLGLRDYESVWQQMKAFTLTRDATTPDEFWQVEHPPVYTLGV
                     SAKPEHLPRTDNGITLVKTDRGGQITYHGPGQVVVYTLLDLRRRHLGVRALVRKLEQA
                     VIELLRDYGLEATGRDNAPGVYVAEAKVAALGLRVRNGCCYHGLSLNVDMDLTPFSTI
                     NPCGYPALEVTQLRALGIRESAPIIADRLLEKLGAQLQ"
     gene            complement(3348..3611)
                     /locus_tag="VN496_13235"
     CDS             complement(3348..3611)
                     /locus_tag="VN496_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HXF17965.1"
                     /translation="MPPESLIEYPTDFPIKIMGRRQPRLVQSIVEVVQRHAPDFDAST
                     VEMRTSKKNNYLSVTCTVLATSREQLDALYRDLCDHPSVVMVL"
     gene            complement(3589..>4026)
                     /locus_tag="VN496_13240"
     CDS             complement(3589..>4026)
                     /locus_tag="VN496_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXF17966.1"
                     /translation="ISLLPNVLLRQQAVEAGCVETVLLRDGFMTEGAASNIFVVNDGI
                     LLAPPKNHLILPGITYDVVLELARANGFRHEVREIPESELRGAEEIWLTSSTKEVLAV
                     TQLDDKPVGKGKPGHVFSRMYALYQDYKNQVMRMAEHATRVID"
BASE COUNT          864 a         1111 c         1142 g          909 t
ORIGIN      
        1 caccaggtcg tactgcttgg ctttgagccg ggaaataaca tccgagacag accccatggt
       61 cgacgggtgc atgatggctt cgcgccacgc ccgatggttg gtcgggatga catcctttac
      121 cgcggggtgc cagcgggcaa tttcctggaa gttttcatcg attgcccaat cgaattcgat
      181 ctgcgggtga gcaagcgtcg cgtcactcag cgccgggagc gcgtggatca gatcccccat
      241 ggacgtcagc ttaattaaca ggactctctt catttttcct tgaaaagagt ctcttgagga
      301 acgcgctgaa acgatgccgc gacaggtgac cccgacgcgc ctcgtcggac gtgacatagt
      361 tgagttgtac cgaccgccgt tcgccaatgt aaatctcgtg cccgtgccag caatcgggtg
      421 tgaccttgaa aatcacgcaa tgccctgcct caggcgaaat ttctgcagca tatggagtga
      481 ggtccctgtt gttgtacagg agtcgcagcc tgccgccgga agccgtccat cccggattta
      541 agtagagcag cagggtgacg agcttggact tcgaatcggt gtgaatgtca ccatccttgg
      601 tatccgtgtg accgcgcaat gtaatcatcg ccggcttgtc gctgagatcc ataccgaggc
      661 gctcaccgat taactcgcgc aattcggtgc tatgcatttc ctccatcagc gctgcaaagg
      721 tacccgagct ggacacggca tcgagcggca cgcttccccg cttgggaaca acgggaaagc
      781 tcttgaccac gtccgcgagc gcttccggaa caatgaagtt gtcgatgatg agatgtggat
      841 acggttccgc gatgacagcc gcctttttta gggcatcggc gttgatgact ttccaggaat
      901 ggggcggggc caaagggatt cctttctgat tacgaaatca gcgagagccg tattttacgc
      961 cctgcatcga ttgctttcgg cctctcatgc gggtcaggaa ccttggcgct aagccgcgta
     1021 tcctactgag acatgaaacg cgaccgtgga aatgccgcgt agaattgcgg gtcgaatact
     1081 gcaaatgtag gtgaaaaggc atcaacgatg gacgtaggaa agaaattcac gctgttcgcc
     1141 attgttctgg cactgaccgc tctggcagcg ggcatctatg ccggatggag ccgccccata
     1201 gcgccggccg atataaattt tatcggtgtc aaaggcgacc ggttccatat gtcgtccttc
     1261 aaaggcaagg tcgttctggt caatttctgg gcgacgtcct gcacagtttg tatcggtgaa
     1321 atgccgaaga tggtgcaaac ctatcagaag taccgtgatc aggggctcga aacggttgcg
     1381 gtcgcgatgt cctatgatcc gcccgaccgc gtgtttgctt atgcgcaaca gaacaaattg
     1441 ccgttccacg tgactctgga tattttcggc acggccatgc acgcctttgg cggcatacgc
     1501 gggaccccga ctacttttct ggtagatcgc cgtggcaata tcgtgaaacg gttcgaaggc
     1561 gaaccggatt tcgcggaact cggcgaactg attgagaaag agctgctgaa agcttaaaca
     1621 tgtccggaac gggacatgcg gcttgacttt taccgagatc agcttacgcc agcttcgtga
     1681 gcctgctggt cagcatgata gctcgaacgc acaagcggcc cgcacgcggc gtgacggaac
     1741 cccatggcca gggcttcctg ctcaaattgc tcaaagcgcg cgggctcgac gtagcgcagc
     1801 accggcaaat gaccgcgcgt aggttgcaga tactgcccta acgtaagcat ctcgacgtca
     1861 tgttcacgaa gatcgcgcat gacttgcagg atctccgcgt cggtttcgcc cagccccagc
     1921 atcaatcctg atttggtcgg aatgtccgga caaacttgct taaagtcccg cagtagcttg
     1981 agcgaatgca ggtagtcggc tccagggcga gcctgtttgt acagccgcgg gacggtttca
     2041 aggttatggt tcattacgtc cggcggatgg gcaaacaacc tgtccagggc gattgtcatg
     2101 cggccgcgaa aatcaggcac gagaatctca atgcgcgttt gcgaagacat ttttcgcacg
     2161 gcgcggatgc actcggcaaa atgttgcgcg ccgccgtcgc gcaggtcgtc ccggtcgacg
     2221 ctggtgatca cgacatactt gagtttcatc gccgcaatgg tccgcgcgag attgcccggc
     2281 tcttgcgagt cgggcgccaa cggccggcca tgggcgacat cgcaaaatgg acagcggcgc
     2341 gtacacaagt cgccgaggat catgaaggtt gccgtgcctt tgccgaagca ttcgccaatg
     2401 ttgggacatg aagcttcttc gcacaccgtg tgcaaattat gttcgcgtaa ggcttgcttg
     2461 atctcctgaa agcgcggact acccggtgct ttgacgcgta tccaatccgg ctttttgagt
     2521 ggaatgattg gaacgatctt gaccggattg cggccagtct tttgttcacc ggtctgtcgt
     2581 tcgcttttgg taggtgtagt cactgaagtt gagcgccaag tttttccagc aatctgtccg
     2641 caatgattgg agcggattca cgaatgccca gcgcgcgaag ttgggtaact tcgagtgcag
     2701 ggtaaccgca ggggttgatg gtactgaagg gggtcaggtc catgtcgacg ttcaggctga
     2761 gcccgtgata gcagcagcca ttgcgtaccc gcagtcccag ggcggccact ttcgcctcag
     2821 caacatatac gcctggtgca ttgtcgcgac cagttgcttc caagccataa tcacgcagca
     2881 attctatcac cgcctgttcc agcttccgca cgagagcgcg cacgccaaga tggcgacgcc
     2941 gcagatcgag cagcgtgtag accaccacct gccccggtcc gtgataggta atctgaccgc
     3001 cgcgatcagt cttgacgagc gtgatgccat tgtccgtgcg tggcaggtgc tcaggctttg
     3061 ccgagacgcc aagcgtatac accggcggat gctcgacttg ccagaactca tcgggcgtcg
     3121 ttgcatcgcg cgtgagcgta aaagctttca tttgctgcca tacggactcg tagtcgcgca
     3181 agccgagccg cctgatggtg aacggtgaac ggtaaacggc aaacggtaaa actgagtcag
     3241 ccggcttggt aactggctct gcctgcggag aattggctat ttcgttgttc acgaccgtac
     3301 cttttcatcg aaaccagtca ctgttaaccg atcaccgatc accgatttta gagcaccatg
     3361 accacggaag gatgatcgca aagatcgcga tacagggcgt cgagctgttc acgagacgtt
     3421 gcgagtaccg tgcacgtgac gctcaaatag ttgttcttct tgctggtgcg catttccacc
     3481 gtcgatgcat cgaaatcggg tgcatggcgc tgcaccactt ccacgatgct ttgcaccagc
     3541 ctgggctggc gccttcccat gatcttgatg ggaaagtcgg tgggatattc aatcaatgac
     3601 tctggtggca tgttcagcca ttcgcatgac ctggttcttg taatcctggt aaagcgcata
     3661 cattctggag aacacgtgac ccggcttacc cttgccgacc ggcttgtcat ccagctgcgt
     3721 tactgcgagc acttccttgg tggaagaagt cagccatatc tcctctgcac cgcgcaattc
     3781 ggattccgga atttctcgta cctcatggcg aaaaccattc gcgcgcgcca attcgagtac
     3841 tacgtcatag gtgatgcccg gcaatatcag gtggtttttg ggcggagcca acagaattcc
     3901 gtcattgacc acgaaaatat tgctagccgc cccctcggtc atgaaaccgt ctcgtagcag
     3961 caccgtttcg acgcacccgg cttccaccgc ttgctggcgc aacagtacat tgggcagtaa
     4021 cgagat
//
LOCUS       DATMZQ010000418        12705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4309823, whole genome shotgun sequence.
ACCESSION   DATMZQ010000418 DATMZQ010000000
VERSION     DATMZQ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4309823"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VN496_13245"
     CDS             complement(<1..837)
                     /locus_tag="VN496_13245"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HXF17967.1"
                     /translation="MPRNLVPPIAPRSPVVNEVHGERRVDDYHWLRNKGSKAVTAYLN
                     AENAYTDAMMKPARALQATLYREMVARIKETDVEVPYRDGDYFYYSRTRKGKQYRILC
                     RRHGSVDAPEQIVLDMNREAKGHKFYSLGAFDVSPDGTLLAFSSDTTGFRQYTLQFKD
                     LRDDRILPDRIEKVRSIAWASDNRTLFFVREDEAKRAYRVYRHLLGSSHTQLIYEESD
                     ARFSVSVSRSRSNEYVFLTSHSATASEVRSLRTDEPQGDFALILARRPEHEYYVDHGH
                     GKF"
     gene            947..1126
                     /locus_tag="VN496_13250"
     CDS             947..1126
                     /locus_tag="VN496_13250"
                     /inference="COORDINATES: protein motif:HMM:NF023369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HXF17968.1"
                     /translation="MQNYESEVTQFIRDFLKKHPEVVEKQRKARATWWDHPQDFKERS
                     RLEAAELPKKSYEYY"
     gene            complement(1147..2181)
                     /locus_tag="VN496_13255"
     CDS             complement(1147..2181)
                     /locus_tag="VN496_13255"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXF17969.1"
                     /translation="MTTSTVLRGVLAAFFLMSGWSSFALADVVNLKNGDRLTGTIVRK
                     EADELVLQTSYAGELKIRWQDVQQLISEQPINLYLDDGRHFKGTTAIGADGQMTIQPA
                     GDVSAAQFDVAQVRWINPSPDVSGEGTKWQGRFNVGAAETSGNTSTEKLHVDAEMIAR
                     TLKNRYTARLEVNHGKSQGEVNESNSLGYLKYDRFLSPKWYAYLNGSAERDRFKDIEL
                     RSVLGAGSGYQFFETPRTNLALEAGISNVHTDFILAPDENYPAARWALKYEQFVGGTK
                     LQFFHRQEVLVGIADVNRTFFRSMTGLRMPVLEKVDATLQYNLDWDNNPAPGRVSADR
                     ALLFTLGYQF"
     gene            2399..3070
                     /locus_tag="VN496_13260"
     CDS             2399..3070
                     /locus_tag="VN496_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17970.1"
                     /translation="MLCRVIFAAVLWQALLGVAVAEEAIPIEQEPRHHMKFANEHVRF
                     FDVELEPGYESRYHWHRSDGVFVNISSSPTIAQDLGKEPVRRGERAIGETYFIDYGAH
                     PKAHRVTNPGSAPYHVVDTEILHGCDATAPWSEGPNQTLLIENPRVFVTRLILHPGES
                     TELYAPCGVLVAVSAGTVNLQSTAGVESVTLQPAGFRWRQQNDTVKLTNTGNFVFHGV
                     DIRLK"
     gene            3089..4294
                     /locus_tag="VN496_13265"
     CDS             3089..4294
                     /locus_tag="VN496_13265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17971.1"
                     /translation="MNNNRKNVFALAACQSLLVMNNAMMISVGTLAADALAQNKMLVT
                     LPATGYIIGGGLTTLPISLFMKRHGRRAGFTIGCIFGMIGATLAAAAMLSHNFWMLCL
                     AALVSGVYTGSGGFYRFAAADMASAGNRSKAISLVLAGGIIGGIFGPESSKVTKDLLD
                     TVFAGSFVSLVALALIALLIVRRLDLPNPSIEEQHGSTRPLAEIMRQPVFIVALLGGI
                     TAYGVMNLLMAATPLAMKMCSHPYGAAMLVIEWHIIAMFAPAFGTGWLIVRLGTLPVM
                     FLGVLLKVAAIAIAVSSTAVPAFWSSMVLIGVGWCFLFVGGTTLLTEACAPAEKAKTQ
                     GINDMLIYVTMASTSLTSGAILYGFGWNTLNYTALPLLCITAIAILWLATIRRSARLR
                     SAAVGVSLP"
     gene            4291..5316
                     /locus_tag="VN496_13270"
     CDS             4291..5316
                     /locus_tag="VN496_13270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXF17972.1"
                     /translation="MSLRLSILDQSPIISGGSSADALRATVALAQRADELGYHRYWLA
                     EHHNMRGLADPCPEILLGHVASATQRIRVGTGGVMLPYYSPLKIAEVFRMHEALHPHR
                     IDLGIGRAPGGDRMTANAMNAHAFDDMEGFPRQVVEVVGWLDRTLPEEHPYASVQAMP
                     SGLSSPEVWLLGSSDYSGALAAYLGLRFAFAHFINAQGGDAVARAYRTDFRPSLRESM
                     PCAMVCVFALCAQTDVEAQRLAASIDHRRLLMAIGREAPIATIQEAHAYPYTERDRAI
                     IERERARAVIGTPDKVKARLIEIADQFSADELMVITITGDYASRMRSYELLAAEFALD
                     PAARLVA"
     gene            5313..6512
                     /locus_tag="VN496_13275"
     CDS             5313..6512
                     /locus_tag="VN496_13275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF17973.1"
                     /translation="MKEDVPAGERKLVLVLAAIQFTHIMDFMIVMPLGPQLMRVMLIS
                     PQQFGLLVSVYTLTAAVAALGVAFYTDRFDRRTTLLFLYAGFLVSTLLCGIAPGYGAL
                     LVARAFAGAFGGVAGATVHSIIGDVVPEKRRGAATGLIMSSFALASIIGVPIGLVLAA
                     HFSWRWPFLFLVAVSSLVLIVSWRVIQPMRGHIVDGVSHRPLAQMKAVLGTSNHLRAF
                     AFMFALMFAGFPVIPFISPYMVANVGLQETDLPYLYLFAGLATAFSSRYIGKLADRHG
                     KRQIFTLIGLISIAPLLITTNLPRVPVPLAICASVIFMVFVSGRFVPAMALVISSVQP
                     HLRGGFMSINSAIQQLGLGAASLLAGSIIGRGEGGMLTHFWLVGLLAVAATLLAIALA
                     WRVKFVA"
     gene            6557..6934
                     /locus_tag="VN496_13280"
     CDS             6557..6934
                     /locus_tag="VN496_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXF17974.1"
                     /translation="MKSETPAKATDIETLRKLNQDYISSVSASDVRRFEELLADDFLN
                     TNADGSFVNRAQFLAQIAKPMTVSNFQCEDVLIRVMGDFAIIHGRTTYRKADGQSGAG
                     RYTDMWALRDGRWLCVAAHVMRG"
     gene            7031..7642
                     /locus_tag="VN496_13285"
     CDS             7031..7642
                     /locus_tag="VN496_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17975.1"
                     /translation="MLDAGNFPSIREYGLMSASRLIGATEGSSRHASRSHRPESQRLA
                     SGILIRNQKPMPQKALARCLQDGLAPEDWFELLNSKVFFWVDAGRLNRQRRACGTSTQ
                     IVLTVDAARLLARYGAQATVTPINTGNAMRAAAPRNRTTFVPYQRWIKEGWAHEKLGR
                     AATRASSHKPVELTIDDAVPDIMDYVTAVVTLRAGEPFRPGSR"
     gene            complement(7639..8544)
                     /locus_tag="VN496_13290"
     CDS             complement(7639..8544)
                     /locus_tag="VN496_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXF17976.1"
                     /translation="MSKASSLRAVLYALGANTGILIAKGIAAAATGSTAMLAEAIHSA
                     ADCGNQVLLLRGMNEAKREPDAKHPLGYGKVVYFWAFLVAVMLFTLGGLFSVYEGWHK
                     LHQTEPISSPIIAIIVLSVSIVLEAFSLAGCVREIRKVSRGMSLWRFFHVSRSSELII
                     VLGEDIAALAGLSLALLAILLAIATGNPMFDALGSMAVGVLLIVVAVLLSLEIKGLIT
                     GESTEVETEAAIRAFIAARVEVAELFNLLTLQMGDDIMLAVKARMVETGSAAALIEAI
                     NRVEADLRAAFPAVRWCFFEPDTKN"
     gene            complement(8613..9434)
                     /gene="eutC"
                     /locus_tag="VN496_13295"
     CDS             complement(8613..9434)
                     /gene="eutC"
                     /locus_tag="VN496_13295"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HXF17977.1"
                     /translation="MSREPVAPGAIAAVQADPWNALRRFTPARIGLGRAGGSIPTSQL
                     LDFQLAHARARDAVHCEFDIEAFSRKLRETKIECLKLASAAVDRHTYIQRPDLGRILD
                     DTSKALLEHRTPAQAWDAAFLVADGLSARAVERHALPVIHMIMPHLEREGWRIAPCCL
                     VAQGRVAIGDEIGALLPAQLTVMLVGERPGLSSPDSLGAYLTWNPLPGRSNAERNCVS
                     NIRPQGLSYAAAAFKLLHLMRTSRAARASGIALKENAVLPDAIELARLFPHAIDG"
     gene            complement(9438..10814)
                     /locus_tag="VN496_13300"
     CDS             complement(9438..10814)
                     /locus_tag="VN496_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HXF17978.1"
                     /translation="MAGYSCTVGEQRYRFPDLKTLLAKATPARSGDRLAGLAASSEEE
                     RVAAQMALADTPLQNFLAEAVVPYEQDEVTRLIIDSHDAPAFAPIANLTVGGLRDWLL
                     DYDTDSAKLAELAPGVTPEMAAAVSKIMRIQDMVLVARKCIVTARFRDTLGLQGRLSN
                     RLQPNHPTDDPKGIAASILDGLLYGCGDAVIGINPATDNVDQVIRLLVMLDEIIQTYR
                     IPTQSCVLTHVTNTLRAIEQRAPVDLVFQSIAGTEAANASFGLNLALLGEANQAARQL
                     GRGTLGSNVMYFETGQGSALSANAHHGVDQQTCEARAYGVARAFSPLLVNSVVGFIGP
                     EYLNGREIVRAGVEDHFCGKLLGLPMGIDVCYTNHADADQDDMDTLLTLLGTAGCNFI
                     MGVPGADDIMLNYQSTSFHDALYLRQLLGLRPAPEFEAWLEHAGIIENGTRLCEVAAG
                     HPLLGLVR"
     gene            complement(10910..12418)
                     /gene="iaaH"
                     /locus_tag="VN496_13305"
     CDS             complement(10910..12418)
                     /gene="iaaH"
                     /locus_tag="VN496_13305"
                     /inference="COORDINATES: protein motif:HMM:NF005688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indoleacetamide hydrolase"
                     /protein_id="HXF17979.1"
                     /translation="MTKSVNSCFWFGGWLFSALLLLTQFAAIADESIDYHSLSLTQAA
                     QLIRGKKISSTELVEALLQQAESARGLNAFITLDREGALKAARAADAAQQQGRIFGPL
                     HGVPLVIKDNIHVANLPNTAGTPALRNFVPRENAPVVQALLDAGAIVLGKANMHELAF
                     GATSNNATFGAVHNPYDPARFPGGSSGGTAAAIAAGIAPGGLGTDTGGSVRIPAALTG
                     LVGLRPTSGRYSLEGVTPFSHTRDTVGPLARNVGDVVLLDQVISGDMSSVQPADIKKV
                     RLGVARNAFYSNLDPEVARIMARTLEQLRSAGAELVEVDLDDFAATSGKIGATVGYFE
                     VKSDLTAYLSRYQTGVSLNELAENIASPDVKELFDNNVLGEKAPTAAAYQEAMQRYRP
                     RLQQLYAQAFSGGRIDALIFPTTPMPAQPIENSTEVTLNGKKMSTLLVFVANTRPINN
                     AGIAGLSIPMGMTVDGLPVGVEIDGPAGSDRRLLAIGLQMEKILGSVPSPRR"
     gene            complement(12415..>12705)
                     /locus_tag="VN496_13310"
     CDS             complement(12415..>12705)
                     /locus_tag="VN496_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXF17980.1"
                     /translation="YRMSFTNFSNGHSHSFGGQYLELVPNERIRHTDKFDDPNLPGDM
                     RTTISLKQVFCGTDLSVVQEGIPEAIPPEACYLGWQESLTLLAQLIEAEIPG"
BASE COUNT         2534 a         3829 c         3705 g         2637 t
ORIGIN      
        1 gaacttgccg tggccgtggt cgacgtaata ttcatgttcc ggccggcgcg cgaggatcaa
       61 cgcaaagtcc ccttgcggct cgtcggtacg caagctgcgg acttcggacg ccgtggcgct
      121 gtgggatgtc aggaacacgt attcgttgct tcgactgcgc gaaacggaaa ccgagaatcg
      181 cgcatcgctt tcctcgtaaa tcaattgagt gtgcgagctt cccaacaaat ggcgatagac
      241 tcgatacgct cgcttggcct catcttccct gacaaagaac agcgtgcgat tgtcggatgc
      301 ccacgcgatc gagcgcactt tctcgatgcg atctggcaat atccggtcgt cccgcagatc
      361 cttgaactga agcgtgtact ggcggaaccc tgttgtatcc gacgagaatg caagcagggt
      421 tccatcgggg ctgacgtcga aggcacccag cgaatagaac ttgtgtccct tcgcttcccg
      481 gttcatgtcg agcacaatct gctccggcgc atcgacgctg ccgtggcgac ggcacaagat
      541 gcgatactgc ttgcccttgc gcgtgcgcga atagtagaag taatcgccat cgcggtaggg
      601 cacttcgacg tcggtctcct tgatacgcgc caccatttca cgatacaacg ttgcctgcag
      661 cgcacgcgca ggtttcatca tggcatccgt gtaggcgttc tcggcgttga gataagccgt
      721 aacggccttg ctgcctttgt tgcgtaacca atgatagtca tccacgcgcc gttcgccgtg
      781 tacttcattg acgaccggcg agcgcggggc gattggcggt actaaatttc tgggcactgc
      841 tggactcctg gtggataatc gggaaaactg acctgccagg ttataaaata tggggcggtc
      901 gaaggaaata tccaattcta gccgctcagt ttagaggtaa agcagcatgc agaattacga
      961 atcggaagtg acccaattca ttcgggattt cctaaagaaa catcctgaag tcgtggagaa
     1021 gcaaagaaaa gcccgggcca catggtggga tcatccgcag gatttcaagg aacgcagccg
     1081 ccttgaagcg gccgaactgc ccaagaaaag ctacgagtac tactagaaat ccttcccccg
     1141 gccggcctaa aactggtaac ccagggtaaa cagcagggca cgatcggcac tgaccctgcc
     1201 cggggcaggg ttgttatccc aatcaaggtt gtactgcaag gttgcatcga ccttctccaa
     1261 aaccggcatg cgcagtccgg tcatggatct gaaaaaagtc cggttgacat ctgcgatgcc
     1321 aaccaacact tcttggcgat gaaaaaactg cagcttggtg ccgcccacaa actgttcgta
     1381 tttaagcgcc cagcgtgcgg caggatagtt ttcgtccgga gccagaatga aatcagtatg
     1441 cacattgctg atacccgctt ccagtgccag attggtgcgc ggcgtctcga agaactgata
     1501 accagaaccc gcgcccagga cagagcgtag ttcgatgtcc ttgaatcggt cacgttccgc
     1561 actgccgttc agatacgcat accatttggg gctcaggaac cggtcatatt tcagatagcc
     1621 gagcgagttc gactcgttta cctccccctg gcttttaccg tggttgacct ccaaccttgc
     1681 ggtgtacctg ttcttcagcg ttcgcgcgat catctccgca tcgacatgca gtttctctgt
     1741 actcgtattg ccggaagtct ctgcagctcc gacgttgaat ctgccttgcc acttggttcc
     1801 ttcgcctgaa acatccgggg acggattgat ccagcggacc tgcgccacgt cgaattgcgc
     1861 ggcagagaca tcgccggctg gctgtatggt catttgtccg tcggcgccaa tcgcggttgt
     1921 tcccttgaag tgtcggccgt cgtccagata gagattgatt ggctgttcgc tgattagctg
     1981 ctggacgtcc tgccagcgga ttttaagttc acccgcgtat gaagtttgca acaccagctc
     2041 atccgcctcc ttgcggacaa tggttcccgt taaccgatcg ccgtttttca aattgacaac
     2101 atcagccagc gcaaaactgg accagcccga cattaaaaag aatgcagcca gcacgccgcg
     2161 cagaacagtg ctcgtggtca attgttgtgc tcccgggtta ttcgttgttc tgcaggcacc
     2221 ataaggcgat gcccgccata ggggcgggcc gctaacgaac agtgatgcct ttaacgggcc
     2281 ggagttccct tgaagataac gtcatgcaaa acgcctgccc tggcgtcgaa caaagtccca
     2341 cgcaaacctt tctggcagaa tgtcgcgctt ggccgcaaac ttttccagga gtcgttgcat
     2401 gctgtgtcgt gtcatctttg ctgctgtgct gtggcaagcc ctattgggcg tcgcggtggc
     2461 tgaagaagcc attccgatcg aacaggagcc gcgccaccac atgaagtttg ccaacgagca
     2521 cgtgcgcttc ttcgatgtgg aactggagcc cggatacgaa tcgcgctatc actggcatcg
     2581 cagtgacggt gtcttcgtca acatttcgtc ctcgccgacc attgcgcagg acttgggcaa
     2641 agagccggtt cggcgcggtg aacgggcgat cggcgaaact tactttatcg attatggggc
     2701 gcatcccaaa gcccatcgcg tcaccaatcc tggcagcgca ccgtatcacg tcgtcgatac
     2761 cgaaatcctt cacggttgcg acgctacagc gccctggtcc gagggcccga accagacgct
     2821 gctgattgaa aatccgcggg tattcgtcac gcggctgatc ttgcatcccg gcgagagcac
     2881 cgagctttac gcgccatgcg gcgtgctcgt tgcagtctca gctggaactg tcaacttgca
     2941 aagcacggca ggcgtcgaaa gcgtaaccct gcaaccggcc ggattcagat ggcgccagca
     3001 gaacgacacg gtgaagctga ccaataccgg gaacttcgta ttccacggcg tcgacatccg
     3061 gctcaagtag ccgaggagtc gatttcggat gaacaacaac cgcaagaacg tcttcgcgct
     3121 ggcagcatgc cagtcgttac tggtcatgaa caacgcgatg atgatttcag ttgggacgct
     3181 ggctgcggat gcactggcgc aaaataaaat gctggtcacg ctacccgcca cggggtacat
     3241 catcggcggc ggtctcacga ccctccccat ctcgctgttc atgaagcggc acgggcgccg
     3301 tgccggattt accatcggct gcatattcgg catgatcggc gcaacgctgg ccgcggcggc
     3361 catgctgtcg cacaatttct ggatgctgtg cctcgcagcg ctggtgtccg gggtatacac
     3421 cgggtcgggc ggcttctatc gtttcgctgc tgcggacatg gcttcggccg gcaaccgaag
     3481 caaggccatt tcgctggttc tggcgggcgg catcatcggc gggatattcg gtcccgaaag
     3541 cagcaaggtc accaaagatc tgctcgatac ggtattcgcg ggctcgttcg tttcactggt
     3601 ggcactggcg ctgattgcct tgttgatcgt gcgccgtctc gatttgccga atccctcaat
     3661 cgaagagcag catggcagca cccgcccgct ggctgaaatc atgcgccagc cggtgttcat
     3721 tgtcgccttg ctcggcggca ttaccgccta tggcgtgatg aacctgctga tggccgctac
     3781 accgctggcg atgaagatgt gttcccaccc gtatggcgca gccatgctgg tcatcgagtg
     3841 gcacatcatc gccatgttcg cgccggcatt tggcaccggt tggctgatcg tgcgcctcgg
     3901 cacgttgccg gtcatgtttc tcggcgtgct gctaaaagtt gccgcgattg cgatcgccgt
     3961 ttccagcact gccgtgccgg ccttttggtc gtcgatggtg ctgatcggcg tgggctggtg
     4021 cttcctgttt gtgggcggta ccaccttgct gaccgaagct tgcgcgccgg ctgaaaaagc
     4081 caagacgcaa ggcatcaatg acatgctgat ctacgtgacc atggcctcga cttccctgac
     4141 ctcgggagcc attctttacg gattcggctg gaacacgctg aattacaccg cgctgccatt
     4201 actttgcatc accgcgatcg cgattctgtg gctggcaacg atccggcgca gcgcgcggtt
     4261 gcggtccgct gcagtcggcg tgtcgttacc gtgagcttgc gactcagcat cctcgatcaa
     4321 tcgccgatca tcagtggcgg ctcttccgct gacgcgctgc gcgcaaccgt tgcgctcgcg
     4381 cagcgcgccg atgaacttgg gtaccaccgc tactggctcg cggaacatca caacatgcgc
     4441 ggccttgccg acccgtgtcc ggaaatcctg ctcggccatg tcgcgtcggc cacacagcgt
     4501 attcgcgtcg gcacaggcgg cgtcatgctg ccctactaca gcccgctgaa aatcgctgaa
     4561 gtattccgca tgcacgaggc actgcatccg caccgcattg acttgggcat cggccgggcg
     4621 cccggtggcg atcgcatgac cgccaacgcg atgaatgcgc acgccttcga tgacatggaa
     4681 ggcttcccaa ggcaggtcgt ggaagtagtc ggttggctcg accgcacatt gccggaagaa
     4741 cacccgtacg ccagcgtgca ggccatgcca tcaggcttaa gttctcccga agtatggctg
     4801 cttggctcct cggattacag cggcgcgctg gccgcctatc tgggattgcg tttcgcattc
     4861 gcgcatttca tcaacgcgca gggaggcgac gcagtagcgc gtgcttatcg cacggatttc
     4921 cgtccatcgc tacgggaatc catgccttgt gcaatggtct gcgtgttcgc gctctgcgcg
     4981 cagacggatg tcgaagcgca gcggcttgca gccagcattg accaccgccg cctgttgatg
     5041 gcgatcgggc gggaagcgcc gattgccacg atccaggaag cgcatgcgta tccgtacacc
     5101 gagcgcgacc gcgcgattat tgaacgagaa cgcgcacgcg ccgttatcgg cacgccagac
     5161 aaggtaaaag cgcgcctgat cgagattgcc gatcaatttt ccgcggatga actcatggta
     5221 atcaccatca ccggcgatta tgcgagccgc atgagatcct atgaactact ggcagccgag
     5281 tttgctcttg atccggccgc aaggttggtc gcatgaagga agatgttccg gccggcgaac
     5341 gcaagctggt cctcgttctt gccgccatcc agttcacgca catcatggat ttcatgatcg
     5401 tgatgccgct gggtccgcaa ttgatgcgcg tcatgctgat ttcgccccag caattcggcc
     5461 tgctggtttc cgtctatacc ttgacggcgg cagttgcagc gctcggcgtc gctttctaca
     5521 cggaccgttt cgaccgccgc accacgctgc tgtttcttta cgcggggttc ctggtgtcga
     5581 cgctgctgtg cggcatcgca cccggttacg gcgcgttgct ggtagcacgc gcgtttgccg
     5641 gggcgttcgg cggtgtggcc ggcgcaaccg ttcattccat catcggcgac gtggttccgg
     5701 aaaaacgccg cggtgcggcg accggtctga tcatgtcttc gttcgcgttg gcttcgatta
     5761 tcggcgtgcc gatcggattg gtgcttgcgg cacacttttc ctggcgctgg ccgttcctgt
     5821 ttctggtcgc cgtgtcgtcg ctggtattga tcgtaagctg gagagtcatt caacccatgc
     5881 ggggccacat tgtggacggc gtttcgcacc ggcccttggc gcagatgaag gcggtactcg
     5941 gtacttcgaa ccatttgcgc gcattcgctt tcatgtttgc gctgatgttc gccggattcc
     6001 cggtgattcc gttcatcagc ccgtacatgg tcgccaatgt cggactgcag gaaaccgacc
     6061 tgccttacct ctatctgttc gcagggctcg ccaccgcatt ttcctcccgc tacatcggca
     6121 agctcgcgga ccggcacggc aagcggcaga tttttacgct gatcggcctc atctcgatcg
     6181 ctccgctgct gatcacgacg aatcttccgc gcgtcccggt cccactggcg atctgcgcat
     6241 cggtcatatt catggtgttc gtgtccggac gtttcgtgcc ggccatggcg ttggtgattt
     6301 caagcgtaca gccgcacttg cgcggtggct tcatgagcat caattcggcc atccagcaac
     6361 tcggactcgg tgcagcctcg ctgcttgccg ggtccatcat cggtcgtggc gagggtggca
     6421 tgctgacgca tttctggctg gtgggacttc tcgccgtggc tgcaacgttg cttgcgatag
     6481 cactcgcgtg gcgagtgaaa ttcgtagcgt agggctcgtt agaattcatg catcacaaca
     6541 ggaacgggag catccaatga aatcagaaac gcccgcgaaa gcaaccgaca tcgaaacact
     6601 gcgcaaactc aaccaggatt acatcagttc ggtatcggca tccgacgtac gacggtttga
     6661 agagctgctg gcggacgatt tcctcaatac caacgccgac ggctccttcg taaaccgcgc
     6721 ccaattcctc gcgcagattg cgaaaccgat gacggtatcg aacttccagt gcgaagacgt
     6781 gctgatccgg gtgatgggcg atttcgcgat cattcacgga cgcaccacgt accggaaggc
     6841 cgacggccag tcgggtgcgg gccgctacac cgacatgtgg gcattgcggg acgggcgctg
     6901 gctttgcgtc gccgcccatg tcatgcgcgg ttgaatgctg cctgatcacg accaatctga
     6961 acggttttat gctggattga cgtaacgtct gagagccatc atgccccgcg ggatacggac
     7021 tgcgtttcac atgctggatg cgggcaactt cccgtcaatc cgcgagtacg gtctgatgag
     7081 cgcctctcgc ctgatcggtg cgaccgaggg cagtagccgg cacgcgtcac gatcgcatcg
     7141 acctgaaagc cagcgccttg cctccggcat cctgatccgc aaccagaaac cgatgccgca
     7201 aaaagctttg gcgcgctgcc tgcaggatgg cctggcgccc gaagactggt tcgaactgct
     7261 caactcaaaa gtctttttct gggtggacgc cggcaggctg aatcgccagc gccgcgcgtg
     7321 cgggacatcg acgcagatcg tccttaccgt ggacgcggcg cggctgctgg ccaggtacgg
     7381 tgcgcaggcc acggtcacac cgatcaatac cggcaatgcg atgcgcgccg ctgcacccag
     7441 gaatcgcacg acgttcgtcc cgtaccagcg atggatcaag gagggctggg cccacgaaaa
     7501 gctgggtcgg gcagcaacgc gcgcgtcgag tcataaaccc gtggaactga ccatcgatga
     7561 cgcggtgccc gacatcatgg actacgtgac cgccgtcgtc accctgcgcg ccggcgaacc
     7621 gttccggccc ggctcgcgct agttcttggt gtccggctcg aagaagcacc agcgcaccgc
     7681 gggaaacgcg gcccgcagat cggcttcgac gcgattgatg gcttcgatca gcgctgctgc
     7741 gctgccggtt tcgaccatgc gcgccttgac ggctagcatg atgtcgtcgc ccatttgcag
     7801 cgtcagcaga ttgaatagtt cggcgacttc gacgcgcgcc gcgataaaag cgcggattgc
     7861 cgcctcggtt tcgacctcgg tgctctcccc ggtaatcagc ccctttattt ccagggacag
     7921 cagcacggcc accacgatca gcaatacgcc aacggccatg ctgcccagag catcaaacat
     7981 gggattgccg gttgcaattg ccagcaatat cgcgagcagc gccagcgaaa gtccagccaa
     8041 tgcggcaatg tcttcgccca acacgatgat gagttcgcta ctgcgcgaca cgtgaaagaa
     8101 tcgccagagc gacatgcccc tcgagacctt gcggatttcc cgtacgcagc cggcaagcga
     8161 aaacgcctcc agtacgatcg aaacgctcag tacgataatg gcgatgatcg gactggaaat
     8221 cggctcggtc tgatgcagtt tgtgccaccc ttcatagacc gaaaacaagc cgcccaacgt
     8281 gaatagcatc acggcaacca gaaacgccca gaagtaaacg accttgccat agccaagcgg
     8341 atgcttggca tccggttcgc gctttgcctc attcatgcca cgcaacaaca acacctggtt
     8401 gccgcaatcc gccgcggaat ggattgcttc ggccagcatg gccgtcgacc cggtcgccgc
     8461 cgcagcaatg cctttcgcga tcagaatccc ggtattcgcg cccagcgcgt agaggaccgc
     8521 gcgcagtgac gatgctttag acatgattgc ttattgacaa agttatggga aagtttggac
     8581 gcgaagggtg ctcgcaaaag tgctgaaacg cctcaaccgt cgatggcatg gggaaacaat
     8641 cgggcaagtt caatcgcatc cggcagcacc gcattttctt tcagcgcaat accggaggct
     8701 ctcgcagcac gtgaggtccg catgaggtgc aggagtttga acgcagcagc agcgtagctc
     8761 aagccttgcg ggcggatatt cgaaacacaa ttgcgctccg cattgctgcg tcccggcagt
     8821 ggattccagg tcagatatgc gcccaggctg tcgggcgaac tcagtccggg gcgttcgccg
     8881 accagcatga cggtcagctg ggccggcaat agcgcaccaa tttcgtcgcc gatcgcgacc
     8941 cgcccctggg ccaccaggca gcagggcgca atgcgccagc cctctcgctc gagatggggc
     9001 atgatcatgt ggatcaccgg gagggcatgg cgctcgacag cgcgcgcaga taatccgtcc
     9061 gcaaccagga acgccgcatc ccatgcctga gcaggcgtcc ggtgctccag caacgccttg
     9121 gaagtgtcat cgaggatgcg tcccaagtct ggtcgctgga tgtacgtgtg ccggtcgacc
     9181 gctgcactgg caagtttcag gcattctatt tttgtttcgc gcagtttccg gctgaacgcc
     9241 tcgatatcga actcgcaatg taccgcatca cgtgcacgcg catgcgcgag ctggaaatcc
     9301 agtaactggc tggtggggat gctgccgccg gcacgaccca ggccgatgcg tgccggcgtg
     9361 aagcggcgca gcgcgttcca cggatcagcc tgaacggcgg cgatggctcc tggcgcgaca
     9421 ggctccctgg acatggctca gcgcaccagg cccagcagtg gatgtccggc cgcaacctcg
     9481 catagccgcg tgccgttctc gataatgccc gcgtgttcca gccacgcttc gaactccggc
     9541 gctggtctaa gacccagcag ttggcgcaag tagagcgcat cgtgaaaaga cgtgctctga
     9601 tagttcagca tgatgtcatc ggcgcccgga acgcccatga tgaaattgca gccggccgta
     9661 ccgagcaagg tcagcaaagt atccatgtca tcctgatcgg cgtccgcatg gttcgtgtag
     9721 cacacatcga tgcccatcgg caggccgagc agctttccgc agaagtggtc ctccacgccg
     9781 gcacgcacga tttcgcgccc gttcagatat tccggcccga tgaagccgac gacgctgttc
     9841 accagcagtg gcgaaaacgc gcgtgccacg ccataggcgc gcgcctcgca agtctgctga
     9901 tcgacgccat gatgcgcatt ggctgacagc gcgctgccct gcccggtctc gaaatacatg
     9961 acattgctgc ccagcgtgcc ccggcccagt tggcgcgccg cctgattcgc ttcacccagc
    10021 agcgcgagat tgagaccaaa gctggcattc gcggcttcgg tcccggcaat cgactggaac
    10081 accagatcca ccggtgcgcg ctgttcgatg gcgcgcaacg tattggtgac atgcgtcagg
    10141 acacaagact gcgtcggaat gcggtaagtc tgaataatct catccagcat gacgagcagc
    10201 cggatgacct gatcgacatt gtccgtggcg ggattgatgc cgatcacggc atccccgcaa
    10261 ccatacagca gcccatcgag aatgctggcg gcaatacctt tggggtcatc cgtcgggtga
    10321 ttcggctgca ggcggttcga caaccttccc tgcagcccga gcgtgtcgcg aaaccgcgcg
    10381 gtaacgatgc atttgcgcgc caccagaacc atgtcctgga tgcgcatgat tttcgaaacg
    10441 gccgctgcca tttcaggcgt aacgccggga gcaagctccg cgagtttggc agagtcggta
    10501 tcgtaatcga gcagccagtc gcgcagaccg ccgactgtga gattggcaat gggtgcaaag
    10561 gccggcgcat cgtgcgaatc gatgatgaga cgcgtgactt cgtcttgttc gtaaggcacg
    10621 acggcttcag cgagaaagtt ttgcagcggg gtatctgcca aggccatttg cgcggcaacg
    10681 cgctcttctt cactacttgc tgccaggccc gcaagccggt cgcctgaacg cgcaggcgtg
    10741 gctttcgcca acaaggtttt gagatcgggg aaacgataac gctgctcacc aaccgtgcat
    10801 gaatagccgg ccataccgga ggtttaccag aatcggatcg cgaacgacgt gaccatgata
    10861 acccagcgca gcggcagagc ttgccgcccc gcataggcta tacggatgct catcgcctgg
    10921 gcgacggtac cgagcccagg attttttcca tctgcaatcc gatcgccagc aggcgccggt
    10981 cactgccggc cggcccatcg atctccaccc cgactggcaa accatctacg gtcatgccca
    11041 tcggaatgct aagccctgca atgcccgcgt tgttgatggg gcgcgtattg gccacgaaca
    11101 ccagcagggt cgacattttc ttgccgttca gcgtgacctc ggtggaattc tcgattggtt
    11161 gcgccggcat cggtgtggtc ggaaaaataa gcgcatcgat cctgccaccg gaaaatgcct
    11221 gcgcgtacag ttgctggagc ctgggacggt accgttgcat ggcctcctgg tacgccgcgg
    11281 cggtcggcgc cttttcgccg agcacattgt tgtcgaacag ctctttcacg tcgggactcg
    11341 caatgttttc cgccagttcg ttcagtgata ctccggtctg atacctggac agataagcag
    11401 tcagatcaga cttgacttcg aaataaccaa cggtcgcgcc aattttgccg ctggttgcag
    11461 cgaagtcatc cagatcgacc tccacgagtt cggcgcctgc gctgcgcaat tgctccaggg
    11521 tgcgcgccat gatgcgagcg acttccggat cgaggttgga ataaaacgcg ttgcgcgcaa
    11581 cgccaagacg tacctttttg atgtctgctg gctgcacgga agacatgtcg cccgaaatca
    11641 cctggtccag aagcacgaca tcgccaacat tgcgggccag tggaccgacc gtgtcgcgtg
    11701 tatgcgaaaa aggcgtgacc ccttcgaggc tgtagcgtcc tgaagtcggg cgcagaccaa
    11761 cgagccccgt aagcgccgcc gggatgcgca ccgaaccccc ggtatcggtg ccgagcccac
    11821 cgggcgcgat tccggcggcg atcgcggctg ccgtgccgcc gctcgatcca ccgggaaacc
    11881 gcgcaggatc ataaggatta tggacggcgc cgaatgttgc attgttgctg gtcgcgccaa
    11941 atgcgagctc gtgcatgttg gctttgccga gcacgatcgc tcctgcgtcg agcaacgcct
    12001 ggaccaccgg cgcattctcg cgcggaacga agttgcgcaa cgcgggagtc cctgccgtat
    12061 tgggaagatt ggccacgtgg atgttgtcct tgatcaccag tggcacacca tgcaaaggtc
    12121 cgaagattct gccctgctgt tgcgcagcgt cggcggcgcg cgcggcttta agcgcgcctt
    12181 cgcgatccag cgtgataaag gcattcaggc cgcgcgcgct ttctgcttgt tgcagcaacg
    12241 cctcgaccag ttccgtgctg ctgatctttt ttccccggat cagctgtgcg gcttgcgtga
    12301 gtgacaagct gtgataatcg atggactcat ctgcaatcgc agcaaactgg gtcagcaaaa
    12361 gcagcgctga aaacagccag ccaccgaacc aaaagcagct attcacagac tttgtcaccc
    12421 cggaatctcc gcctcgatca gttgtgcgag caaggtcagt gactcttgcc agccgagata
    12481 acaggcctcg ggcggaatgg cttccggtat gccttcctgc acgacgctca ggtctgttcc
    12541 gcagaacacc tgcttcaatg aaatcgtggt ccgcatgtct ccgggcaagt tcggatcgtc
    12601 aaacttgtcg gtgtgccgga tgcgttcgtt tggcaccagt tcgagatact gtccgccgaa
    12661 ggaatggcta tggccgttgc tgaaattggt gaaggacatc ctata
//
LOCUS       DATMZQ010000419         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2750013, whole genome shotgun sequence.
ACCESSION   DATMZQ010000419 DATMZQ010000000
VERSION     DATMZQ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2750013"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VN496_13315"
     CDS             <1..1019
                     /locus_tag="VN496_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HXF17981.1"
                     /translation="TDLGEYIIQQFGETPSHIIAPAVHKSRDDIADLFARKHNRPRLN
                     DITALTHEAREVLRPHFLDADMGISGANFLVAETGSTMIVTNEGNGRMTTTLPRVHVA
                     ITGIEKIVPTLEDIALLLRLLTRSATGQSISNYLSFTTGPKRARDMEGPTEFHVIIVD
                     AGRTRVLASEIREALRCIRCGACMNHCPVYQNIGGHAYGWVYPGPIGSILTPAYVGIE
                     NAIDLPNASTLCNQCGVVCPVKIPLPDLMRKLREQQVRRKLRPWSERFGLTLWAWAAT
                     RPLVYGVLARMAARIGKGLGGRHGLLRKLPGPGSEWTSGRDLPAPRGKTFRELYARRN
                     RSAR"
     gene            1016..1666
                     /locus_tag="VN496_13320"
     CDS             1016..1666
                     /locus_tag="VN496_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HXF17982.1"
                     /translation="MSARENILRRIREANGREAGETAGRRIAIRKKLDDRSRGPLPTM
                     AWEPVPHFKERCIAMMSTVSEVESLDGVPHALASYLKANQLPATGVCWAEFAALNWAG
                     SGLTIEARPSHAEDKLGITGVFCALAENGTLMLLSGETTPATTSLLPETHVAVVPFSR
                     IVRTMEDGWDLLRRERGVLPRQVNFISGPSRTADIEMTLVLGAHGPFRVHVILVTR"
     gene            1804..>2484
                     /locus_tag="VN496_13325"
     CDS             1804..>2484
                     /locus_tag="VN496_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HXF17983.1"
                     /translation="MMILGIPFEFVLFAITLLGVGLFHRHTLNVAVAGLAAVIAYKLT
                     VTGFKNGAGLEGLAAHLGHEWVMLANLFALLVGFALLAGHFHDSRAPDVLPRYLPDDW
                     KGGFVLLLMVFVLSSFLDNIAAAVIGGTIAAAVFRGKVHIGYLVAIVAASNAGGSGSV
                     IGDTTTTMMWIAGVGPTSVLHAYVAAVVAVLFFGVIAARQQQRYAPIAPDPAVGIRVD
                     FVRLGIVVF"
BASE COUNT          496 a          671 c          741 g          576 t
ORIGIN      
        1 agaccgacct tggcgagtac atcatccagc agttcggtga gacgccatcg cacatcatcg
       61 cccccgccgt acacaaatca agggacgaca ttgctgatct ttttgccaga aagcacaacc
      121 gaccccgact caacgatata acggcgctga ctcatgaagc cagggaagtt ttgcgaccgc
      181 acttccttga tgcggacatg ggcatcagcg gagcgaattt tctggttgcc gaaacaggtt
      241 cgaccatgat cgtcaccaat gagggtaatg gccgcatgac cactaccctg ccgcgggttc
      301 acgtggcgat caccgggatc gaaaagattg tgccgaccct ggaggacatt gcgctgcttc
      361 tccgattgct gacccgttca gccaccggcc aatccatcag caattacctt tccttcacga
      421 ccggaccgaa acgggcacgt gatatggagg gtccgacgga atttcacgtc atcatcgtcg
      481 acgcgggacg caccagggtg ctcgcctcgg agattcgcga ggcattgcgt tgtatccgct
      541 gtggtgcttg catgaaccac tgtccggttt atcagaacat cggcggtcat gcgtacggtt
      601 gggtgtatcc cggacccatc ggatcgattc ttacgccggc ctatgtcggc atcgagaatg
      661 cgatcgatct gccaaatgct tctacactgt gcaatcagtg tggcgtggta tgtccggtaa
      721 agattccttt gcctgatttg atgcgcaagc tgcgcgaaca acaggtacgc agaaaattgc
      781 ggccatggtc cgagcggttc ggcctgacac tttgggcctg ggcggctacg cggccgctgg
      841 tctacggcgt actcgcgcgc atggcagccc gaatcggcaa agggctgggc gggcgccatg
      901 gattgttgcg aaaactgcca ggtcctggat ccgaatggac ctccggtcga gatcttcctg
      961 ctccacgagg aaaaacattt cgcgaacttt atgcacgtcg caatcggagc gctcgatgag
     1021 cgcgcgggaa aatattctta gacggattcg tgaagccaat gggcgcgaag ccggagagac
     1081 ggcaggacga agaatcgcca tccggaagaa gctggatgat cgtagccggg gaccattgcc
     1141 taccatggct tgggaaccgg tcccgcactt caaagagcgc tgcatcgcaa tgatgtccac
     1201 cgtgagcgag gtggaatcgc ttgatggcgt tccgcatgcg cttgcgtcgt atttgaaagc
     1261 caaccaactg cccgcaacag gtgtgtgttg ggcggaattt gcggctctga attgggctgg
     1321 ttcggggctc acgatagaag cgcgcccgag ccatgccgag gacaaactgg gcataacggg
     1381 tgtgttttgt gcgctggctg aaaatggcac attgatgttg ttatccggtg aaaccacgcc
     1441 tgcgaccacg agtctgctac cagaaacaca cgtggccgtg gtcccgttct ctcgcatcgt
     1501 ccgcacgatg gaggacggtt gggacttatt gcgccgtgag cgaggcgtgt tgccacgcca
     1561 ggtgaatttc atatccggcc catcgcgtac tgctgatatc gaaatgacgc tggtactcgg
     1621 agcgcacgga ccgttccggg ttcatgtgat attggttacc cgatagatcc gaaagaatac
     1681 agcaggcgtg cggcatctgc ggcagtgcgg aatgcaataa atttgcacgc attggctcgt
     1741 cccggcttca tggcgaaata ttgttttctc agatttgcac caggcgcccg cttaccataa
     1801 cgaatgatga ttcttggcat tccgtttgag ttcgttttat tcgcgataac gttgctgggc
     1861 gttggtctat ttcatcgtca tacgctcaac gtagcggtgg ctggcctggc ggcagtgatc
     1921 gcctacaagc ttaccgtcac cggtttcaag aacggcgccg ggcttgaagg cctagcagcg
     1981 catctgggcc atgaatgggt gatgctcgca aatctgttcg cattgctggt cggattcgcg
     2041 ctgttggctg gccattttca cgatagtcgc gcgccggatg ttttgccgcg ctacctgccg
     2101 gatgactgga agggcggttt cgtgctgctg ctcatggttt tcgtgctatc cagctttctg
     2161 gacaatatcg cagccgccgt tattggcggc acaattgctg ccgcggtatt ccgcggcaaa
     2221 gttcatattg gttatctggt cgcgatcgtg gctgcatcca atgcgggggg ttctggcagc
     2281 gtgattggcg ataccaccac caccatgatg tggatagcgg gcgtgggccc gacttcggtt
     2341 ttgcatgcct acgtcgcggc tgtagtcgcc gtgctctttt ttggtgtaat cgcagcgcgc
     2401 cagcagcagc gctatgcccc aattgcgcca gacccggcgg taggcatccg ggtggatttt
     2461 gtccgtcttg gtatcgtggt tttt
//
LOCUS       DATMZQ010000420        12853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4133418, whole genome shotgun sequence.
ACCESSION   DATMZQ010000420 DATMZQ010000000
VERSION     DATMZQ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133418"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VN496_13330"
     CDS             <1..310
                     /locus_tag="VN496_13330"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXF17984.1"
                     /translation="VERKQLKLPEKNPRQKIKDHCRIRYNKPSMMQHIEQGRRTEIDA
                     LNGALVREARALGVAVPYNEAIVAVVKGLEKSRRQALHEPPRDYKKLEKEAEKEAGKT
                     "
     gene            492..1043
                     /locus_tag="VN496_13335"
     CDS             492..1043
                     /locus_tag="VN496_13335"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HXF17985.1"
                     /translation="MNPEPAGVLGSIWIRPTGNALDSIKHAVRIACENAGGPPVQPHV
                     SLMGGIEITTDIATKLKTLAARIRGFEIRLGKIQWRGEYYRSLFVSVELSDELMAAHL
                     AAHEEFGRLPSAPFEPHVSLLYGDFDETFKKELAKQLGDRLDVRFTAGALDLVNACEK
                     VPIAQWETLHQAMLRSAPIKEKT"
     gene            complement(1063..1377)
                     /locus_tag="VN496_13340"
     CDS             complement(1063..1377)
                     /locus_tag="VN496_13340"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HXF17986.1"
                     /translation="MIVKFDSEAGTFIMNGDVAVQLLRMMGHSGTVPSAILPRDIPGA
                     LTRLKAALHADRGHSDAPTGNDEDEQKELVVSLRQRAFPLIDLLTRAAAKGTEVMWSS
                     QT"
     gene            1562..3919
                     /locus_tag="VN496_13345"
     CDS             1562..3919
                     /locus_tag="VN496_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXF17987.1"
                     /translation="MASPREIEPILLNVPGHIHTFLERLRLLAAQYPFALLLASVIAV
                     LLVALFLYVIIRKLRAGRPWLLGSAASSRAEMSKDGDQYRTLLQNSPIPTLICDTRRN
                     RLLEVNDALVREYGYTREELLKMTLFDLGLPQDKERVSATLNSLGSNSGELGTWPHRT
                     KSGAEVQAEMVTFRMRYGRRQACVVIAKDVTRRKRTHEVMWQDLERYGLASRATNDAL
                     IDWDLVSDALWRSENYAQIFGTGEGSSAPNMAAWFEHIHPDDRARVHEKLLATIKSDA
                     SHWSDEFRLVRGDHKITHAFARGYISRADDGSARRLNAVLQDITERKEQEERTRFLAD
                     HDELTELPNRSLFRQSLNQALQRAARSGKMLSILFFDLDRFKNINDSLGHDAGDEVLR
                     AVAERLTACVRKIDMVARFGGDEFAVLTEGLTAEDQAGTVARKILEVLSKPMILAGRQ
                     YRPAASIGISTYPTDGRDAQALLKNADIAMYRAKEEGRGTFQFYSEQLNTHSVQRLEF
                     ESNLTSALNNREFVLHYQPKVDLATGRITGLEALIRWVSPQLGFVPPGDFISIAEETG
                     LIVPLGRWVAQTACVQNAAWQKAGLPQLRIAINISARQMSDTGLVEFISDTVSKTGLT
                     VQSLELEITESAVMSNQEHAEKVLNRLKALGLHLTMDDFGTGYSSLAYLKRFPFDSVK
                     IDQSFVRGIPQSKDDEAIVEAIIAMAHSLQLEVVAEGVETREQYEFLQSRGCDQIQGF
                     YFSKPIPANEVVMLLYKAMTRDSSATVPAGEPKLGAAKAATSSKA"
     gene            4126..4716
                     /locus_tag="VN496_13350"
     CDS             4126..4716
                     /locus_tag="VN496_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXF17988.1"
                     /translation="MTENSLAQPSSKKELFVAFTVLALQGFGGVLAVAQRVLCEQKRW
                     LSRDQFVEILSLAQILPGPNVCNVALMIGDRFFGWRGAFAALAGMMAVPLVIVLFFTA
                     LYSQYALNPAVAGALRGMGAVSAGLIIGTGLKLVGALRANPMKTPICAALLVAAFAAV
                     ALLRWPLVWVILALGVGACALAWMRLKPRVAAVSRS"
     gene            4713..5240
                     /locus_tag="VN496_13355"
     CDS             4713..5240
                     /locus_tag="VN496_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXF17989.1"
                     /translation="MILSGALLALFGHFLVLSLLSIGGAITVAPDMHRVMVDQMGLLS
                     DAQFNASIAIAQASPGPNVLFVAVMGYQAAGLAGVAATLFGIMLPSTTLALAAARWGH
                     ARREWRAVQAFKAGMAPIVIGLLFSTGWILSAQTPGWHHIIPTIVAALLVWRTRVHLL
                     LLVGAGAFVGAMGWV"
     gene            5441..6166
                     /locus_tag="VN496_13360"
     CDS             5441..6166
                     /locus_tag="VN496_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXF17990.1"
                     /translation="MRYELYYWPSIPGRGEFVRLALEEAGVTYIDVARRSGRSGGVPA
                     MERFLDDAKLAGAPFAPPFLRAGKLIVAQTANILLYLGPRHDLVPRSEAGRVWVNQLQ
                     LTIADFVVEIHDVHHPIAASLYYEQQKEEARRRSKYFRTERIPKFLSYFEQALSAGGR
                     RRHYLAGATRSYADLSMFQLVAGLRYAFPRTMQELEPDCPGLVGLHDRIAERPNIAAY
                     LASPRRIAFNQDGIFRYYPELDG"
     gene            6221..7012
                     /locus_tag="VN496_13365"
     CDS             6221..7012
                     /locus_tag="VN496_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXF17991.1"
                     /translation="MNDKARKLKSVEAGNLRGRVSPEEWEVRCELAAAYQLAAQFRWT
                     DLIFTHFTARVPGSPHILINPYGLMFDEITASSIVKIDHEGNIVDDATGLGINYAGFV
                     IHGCVHEARPEINCVIHTHTRAGVAVSVQNHGLLPLSQHALRTYGMLSYHDYEGIALE
                     LDERARMAADLGPTSKAMILRNHGLLTLGDSVGEAFELMYYLDTACQIQIDALAGGRV
                     EARLIDEKTARKGFDQFKGPGGAEVNKAWQALLRMLDRKGVNYRN"
     gene            complement(7070..7423)
                     /locus_tag="VN496_13370"
     CDS             complement(7070..7423)
                     /locus_tag="VN496_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF17992.1"
                     /translation="MSTVQVDRWVEIARMVFPNGAQITHPPAGESVWFKVRWKVGTDP
                     KRPHKPAKGVNVYFTREFCKAYERLDSGKQSDWDNKVRDMLASKYKEFNPDHNAPHGT
                     PVPVESWFVTPGTVR"
     gene            7537..8358
                     /locus_tag="VN496_13375"
     CDS             7537..8358
                     /locus_tag="VN496_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXF17993.1"
                     /translation="MKNDSLLYEIAPEVMWTIVIVSAVILANITISAVLRGRGWLSRE
                     TKLRASVFWRNFSLLVAFIALIFIWRVELRAAALSLAALSVALVIAGKELFTSVLGYV
                     HRTTSGSFTFGDVIEINNIKGEVIDQTLLSTTVLEMSEEHLFTGRVVQFPNSFYVSNA
                     MKNYSRLGDYQLGMVTIPLAGGTDVETARRILSEVATAVCSEFVAPAHAALRELEGEQ
                     FIVMPSAEPRVSIRLGDGGKTTLLLRFPCPASQRTRTEQELLTRYLAGMRSAAAS"
     gene            8378..9319
                     /locus_tag="VN496_13380"
     CDS             8378..9319
                     /locus_tag="VN496_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXF17994.1"
                     /translation="MSAAAAARKQIICVGHAALDDVFAVDSWPANSGKIAAHGFTRSG
                     GGMAANAASAIARLGGEAVFWGPTGEDPVADAIRESLIDEGVDVHGLRRFAGRTSSHS
                     AVIVDQRGERLVVGYRGSALEVSADWLPLEQIRCAAALLADVRWPDGALVALHAARNA
                     GIPAVLDAEIAPVQTIELLAAAASHVVFSERGLEACCGRDSAAGLRGMLEHGALMAAV
                     TRGAAGVDWVEATGPGKLLHCPAFPVDTVDTLGAGDVFHGAYTLALAEGRLIGEAIRF
                     AAAAAAIKCSRPGGRSGTPNRSEVETFLASAGMKPRD"
     gene            complement(9316..10080)
                     /locus_tag="VN496_13385"
     CDS             complement(9316..10080)
                     /locus_tag="VN496_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF17995.1"
                     /translation="MDAFALDGKVAIVTGSTRGIGRSIAEHLAKAGAKVVISSRKAPA
                     CEEVANSIRAAGGDAIAIPANIGDKTQLEALVTQTRSQCGKIDVLVCNAASNPYYGSM
                     ANMPDDAFNKILQNNIVSNHWLANLVLPEMARRKDGVVIIVSSIGGLKGTSVLGGYAI
                     SKAADMQLARNLAMEWGPQNIRINCIAPGLVKTDFARALWENPEILQRYESQTPLRRI
                     GEPDDIGGIAVFLASRAAAFVTGQTIVADGGVTIAG"
     gene            complement(10145..10684)
                     /locus_tag="VN496_13390"
     CDS             complement(10145..10684)
                     /locus_tag="VN496_13390"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF17996.1"
                     /translation="MKALKALASLIIAALAAPASAAEAPEMSIEHSIVINAPPEAVWQ
                     VVSDFNGLPRWLPVIVESRLILGKNREVGAIRELTRANGTKVQEKLIAYEPWTMSLGY
                     TYIGGQVGATDYFPTITVSDDGGGKSKVVWKARFKRVAYWTDEPPPGQDDATPLNGLN
                     KVYEMGLENLKKVIEEGQQ"
     gene            10929..11777
                     /locus_tag="VN496_13395"
     CDS             10929..11777
                     /locus_tag="VN496_13395"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXF17997.1"
                     /translation="MSPVLSAEELAHYQQRGYVLPRYRLPDPLLQSLRKSLDQLLAAY
                     TDVAQEDLANPHMLPPTSGPDMNPFMAAARHPAILDMVEQIIGPDIVLWIGRILCKPP
                     VKGREVPWHQDGEYWPMRPLATCSVWIAIDPVSVANGCMRFIPGSHQRKELYKHHVSD
                     RKDLVLNLELDSNQFDEREAVNVELEPGQISLHDVRMIHGSLANTSGQRRAALILRYM
                     PGTSHYDRSLRDQRRDKSPFNIYDQPLWLMRGEDKSRKNDLLHGHDIWKRRYASGLGV
                     SSGSAG"
     gene            11863..>12853
                     /locus_tag="VN496_13400"
     CDS             11863..>12853
                     /locus_tag="VN496_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXF17998.1"
                     /translation="MNIANFLVRSARTFPDRPAVSLGDTVWIGYGDLMRRVSAMAAGF
                     RNSLALVPGDRVALAMANCPQYIEIWYAAWYAGLTTVPMNSKLHPKELEFILRDSGAR
                     ICFASSGLTSAVEGLRGELPALERVLDVGSSDYTALYRAHPGIAMHQTAPEGIAWLFY
                     TSGTTGRPKGVMITHRNVLSMSLAYFAAVDTIGPRDCVLHAAPMSHGSGQYNFPHMLV
                     GANQVIPDSGHFDAAEIFDLCRCWTGVTMFAAPTMVHRLVAHASKHAPALDGLKTIVY
                     GGGPMYLQGILNALDTLGPRFAQIYGQGESPMTITALSKAQITDRNQPRYEQRL"
BASE COUNT         2593 a         3647 c         3861 g         2752 t
ORIGIN      
        1 ggtggagcgc aagcaactca agctcccgga gaaaaaccca cgtcagaaaa tcaaggacca
       61 ttgccgtatc cgctacaaca agccgtcgat gatgcagcac atcgagcagg gccgtcgtac
      121 cgaaattgat gcccttaacg gcgctctggt gcgagaggca agggcgctag gcgtcgccgt
      181 tccttacaac gaagcaattg tggcagtggt gaaagggctg gagaaaagcc gtcgtcaggc
      241 gttgcatgaa ccgccccgtg actacaagaa gctcgagaaa gaagcagaaa aggaggcagg
      301 caagacctga caaataagct ggagcctttt ctgcaaaaac gatggttgtg ccacctgatc
      361 ttccaacgtg ccgcaacgaa tggtttcccg gtccgggcgg actatcgctt gctctatagc
      421 gcttgaagac cggatttaat ccccgtccgc tgtgtccaaa cggtttgtcg cttcttgtac
      481 aggagagcat ggtgaaccct gaacccgcag gagtactcgg ctccatttgg atacgtccca
      541 ctggaaacgc gctggactcg atcaaacatg cagttcgcat tgcgtgcgaa aacgccggag
      601 gcccgcccgt gcagccccat gtctctctga tggggggcat tgaaataaca acggatattg
      661 caacgaaact caaaacgctt gctgccagaa tccggggttt tgaaatcagg ctcggaaaaa
      721 tacaatggcg cggcgagtat tatcgttcac tgttcgttag cgtagagctg agcgacgaac
      781 tcatggcggc ccatcttgcg gctcacgagg aattcggacg gctgccgtcc gctccatttg
      841 agccgcatgt cagcctgctc tatggcgatt tcgatgaaac cttcaagaaa gaactcgcaa
      901 aacaattggg cgaccgcctc gatgtccggt ttacggctgg cgcgctcgat ctggtgaacg
      961 cctgtgaaaa ggttccgatc gcacaatggg aaacactgca tcaggcgatg ttgcggtccg
     1021 cgcccattaa ggagaagacc taagccgatg cagtcgggct tttcaggttt ggctgctcca
     1081 catcacttcc gtccccttgg cggcagcgcg ggtcagcaga tcgatcagcg ggaacgcccg
     1141 ctgccttagc gaaaccacta gttccttttg ctcgtcttcg tcgttgccag ttggcgcgtc
     1201 tgaatgtccg cggtcggcat gtagtgctgc cttgagccgg gtaagcgcgc cgggaatatc
     1261 acgaggcagt atcgcactgg gcacggtacc actgtggccc atcatcctga gcaactgcac
     1321 ggcgacgtcg ccgttcatga tgaaggtgcc tgcttcgctg tcgaacttta cgatcatgaa
     1381 gcacctccga ataagattga atcggcgtaa gtttagtttg cgcttccagc ataaagtttc
     1441 cgcgcatcat tcgctcggct actgctcgaa gcgaacccaa ccgccaaatt ctctgcgtcg
     1501 gagcggcagt ttatagggta aattacgcac ccgggccgga ctcattcggg agtgcgaatc
     1561 tgtggcttcg ccgcgggaaa ttgaaccgat actgctaaat gtaccgggac atatccacac
     1621 ctttctggaa cgcctgcgtt tactggccgc tcaatatcct ttcgcgctgc tcctcgccag
     1681 cgtaatcgct gttttgctgg tcgctctttt tttatacgta atcattcgca aactgcgtgc
     1741 tggccggcca tggcttttgg ggagcgcagc gtcctcccgt gctgaaatgt caaaggatgg
     1801 cgaccaatac cggacgctgc ttcagaacag ccccattccc acgttgattt gcgatacgcg
     1861 ccgcaaccgc ctgctcgagg tcaacgatgc cctggtgcgc gagtacggat atacgcgaga
     1921 ggaattactg aaaatgacgc tttttgacct gggtttgccg caggataaag agcgcgtttc
     1981 ggcaacactg aacagcctgg gatctaattc aggtgaactg ggcacgtggc cgcatcggac
     2041 gaaaagcggt gccgaggtcc aggccgagat ggtgacgttc cgaatgcgct atgggcggcg
     2101 acaggcgtgc gtagtcatcg ccaaagatgt tacccgccgc aagcgcacgc acgaagtgat
     2161 gtggcaagat ctggaacggt atgggctggc ttcgagggcg accaacgacg cgctgatcga
     2221 ttgggatctg gtgagcgatg cgttatggcg aagcgaaaac tatgcgcaga tattcggcac
     2281 cggggagggg agttccgcgc caaacatggc agcatggttc gagcacattc atccggacga
     2341 ccgtgcacgc gtgcacgaaa aactgctggc gacaataaag tcggatgcca gccattggag
     2401 cgatgagttt cggctcgtac gcggtgatca caagattacg catgcatttg cccgcggcta
     2461 tataagccgc gcagacgatg gcagtgcgcg cagattgaac gccgtgctgc aagacattac
     2521 cgaacgcaag gagcaggaag agcgcacgcg gttcctcgcc gatcacgacg aattgaccga
     2581 attgccaaat cgcagcctgt ttcgtcaatc gttgaaccag gcgctgcagc gcgccgcgcg
     2641 cagcggcaag atgctgtcga tcctgttctt cgaccttgac cgtttcaaga atatcaacga
     2701 cagccttggt catgacgcag gtgacgaggt cttgcgcgca gtggcggagc ggctgaccgc
     2761 ctgtgtgcga aaaatagata tggtggcgcg tttcgggggg gacgagttcg ccgtcctgac
     2821 cgaaggcctc acggcggaag accaggcagg cacggtggca cgcaagattc ttgaagtgct
     2881 gtcgaagccg atgatcctcg ccggccggca atatcgtccg gcagccagta ttgggatcag
     2941 tacctatccg accgacggac gcgatgctca ggcgctgcta aagaatgccg acatcgcgat
     3001 gtatcgcgcc aaggaagaag gcaggggcac gttccagttt tattccgaac aactgaacac
     3061 gcattcggtg cagcgcctgg aattcgagtc gaacctgacc agcgcgctaa acaatcgcga
     3121 attcgtcctg cactaccagc ccaaggtcga ccttgcgacc gggcgcatca ccggactgga
     3181 ggcactgata cgctgggtca gtccgcaact cgggttcgtg cctccgggcg acttcatttc
     3241 cattgccgaa gagaccggcc tgatcgttcc gctgggccgc tgggtggcgc agacggcctg
     3301 cgtgcagaat gccgcatggc agaaagcggg cttgccgcaa ttgcgcattg caatcaatat
     3361 ttccgcgcga cagatgtccg acaccggttt ggtcgaattc atttccgata ccgtcagtaa
     3421 aaccggcctg acggtgcagt cactggagct cgagatcacc gaaagcgcag tcatgagcaa
     3481 tcaggagcac gcggaaaaag tcttgaaccg actgaaagcg ctggggcttc atctcacgat
     3541 ggacgatttt ggcacgggct attcctccct ggcctatctc aaacgctttc cgtttgacag
     3601 cgtcaagatc gatcagtctt ttgtgcgagg cattccgcaa agcaaggatg acgaagccat
     3661 tgttgaggca atcattgcca tggcgcatag cctgcaactg gaagtggttg ccgagggggt
     3721 cgaaaccagg gaacagtacg agtttttgca gtcgcgtggg tgcgatcaaa tccagggctt
     3781 ttacttcagc aagccgattc cggccaacga agtcgtcatg cttctctaca aggccatgac
     3841 tcgcgattcc tccgctaccg tacctgccgg tgagccaaag ctcggggcgg ccaaagctgc
     3901 gacatcgagc aaggcttaag caccatcaaa ctgaggttca agccggggcc gcgaatgttg
     3961 cgtagtcacc tcagatgaag cggcgcgagt ttgtccgcga cctcctgaca tagggctgga
     4021 ccctacccgg tcacattcgg tatggcattt ccttcgcaat tacggcggcc gtatttatgg
     4081 ttcccggtat gatccccgat tgagatccat gcctccacga cagcaatgac tgaaaattcg
     4141 cttgcgcaac cttcatcgaa gaaagaattg ttcgtggctt ttacggtgct tgcattgcag
     4201 ggatttggcg gcgtgctggc cgtagcccag cgtgtcctgt gcgagcagaa gcggtggctc
     4261 agcagggatc agttcgtcga aatactgtca ctcgcgcaga tacttcccgg ccccaacgtg
     4321 tgcaatgtcg cattgatgat cggtgaccgc ttttttggct ggcgcggtgc ttttgccgcc
     4381 ctggccggca tgatggcggt gccgctcgta attgtgctgt tcttcacggc tttgtatagt
     4441 caatacgcac tgaatcccgc ggttgccgga gcgctgcgcg ggatgggcgc cgtatccgcc
     4501 ggcctgatta tcggcaccgg cctgaaactg gtcggggcat tgcgcgcaaa tccgatgaag
     4561 actccgatct gcgcagccct tcttgttgcc gcatttgcgg cggtcgcgtt gctgcggtgg
     4621 ccgttggttt gggtcattct cgcactcggc gtgggcgcat gtgcattggc ctggatgcgg
     4681 ctcaagccgc gggttgccgc ggtaagccgg tcatgatcct ttccggcgca ttgcttgcgc
     4741 tgttcggtca ctttttggtt ttgtcgctgc tgtccatcgg cggggccatt acagtagcgc
     4801 ccgacatgca tcgggtgatg gtcgaccaga tgggcttgct gagcgatgcg cagttcaacg
     4861 catccattgc catcgcgcag gcgtcaccgg gaccaaacgt cttattcgtg gctgtcatgg
     4921 gctatcaggc ggcgggtctg gccggggtgg cggctacgct gttcggcatc atgttgccct
     4981 ctacgacttt ggcattggcg gcggctcgct ggggccatgc acgacgcgaa tggcgagcgg
     5041 tgcaagcgtt caaggccggc atggccccga tcgtcatcgg attgctgttt tcgaccgggt
     5101 ggatcctgag tgcgcagacg ccgggctggc accatatcat cccgaccatc gtggcggcat
     5161 tgttggtgtg gcgtacgcgc gtgcatctgc tgctgctcgt cggcgcgggg gcgttcgtcg
     5221 gggcgatggg ctgggtgtaa aagaccgtga cggtcgtgac acgaaagcac aagaacgctg
     5281 tgagaacaca cgcgtaagaa ccaagatgcg gtgatagtgt tacggcaaag caggcttgtg
     5341 acttatgctc ttacgtcgtc acgtcgtcac gtcgtcacgt cgtcacgtcg tcacgatagt
     5401 cacgattgtc acggccgtca cggccttatg gagaatatcg atgcgctacg agctttatta
     5461 ctggccttcc attccgggac gcggagaatt cgtccggctg gcactcgagg aggcaggcgt
     5521 gacttacatc gacgttgcgc gccgttcggg ccgcagtggc ggtgtgccag cgatggagcg
     5581 ttttctcgat gacgcaaagc tggccggcgc gccgtttgcg ccgcctttcc tgcgggcggg
     5641 caagctcatc gtcgcccaga ccgccaatat cctgctttac cttggaccgc gtcatgacct
     5701 cgtgccgcga agcgaagcag ggcgcgtgtg ggtcaaccaa ttgcagttga ccatcgcgga
     5761 tttcgtggtc gagatccacg atgtgcacca tcccatcgcg gcttcgcttt actatgaaca
     5821 gcagaaggaa gaagcgcgcc ggcgcagcaa atatttccgg acggagcgca taccgaaatt
     5881 tttgagctat ttcgaacagg ctttatctgc cggcggcagg cggaggcatt acctcgctgg
     5941 cgcgacccga tcgtatgccg acctgtccat gtttcagttg gtggccggat tgcgatacgc
     6001 gtttccccga accatgcaag aactggaacc agactgtccc gggctggtcg gattgcatga
     6061 ccggatcgct gagcgtccca atatcgcggc atatctggcc tcgccgcggc gcattgcatt
     6121 taaccaggat gggatctttc ggtattaccc ggaactagat gggtgatcgc tacagcagcg
     6181 atgggaccag gcgtatgatt gcgacaggag gaatatgcac atgaacgata aagccaggaa
     6241 gctcaaatcc gtcgaggccg gaaatctgcg tggccgcgtc agcccggaag aatgggaagt
     6301 acggtgcgag ctggcggcag cctaccagct ggccgcgcag tttcgctgga cggacctgat
     6361 attcacgcac ttcacggcgc gcgtgcccgg ttcgccgcac atcctgatca atccctacgg
     6421 attgatgttc gacgagatca ccgcatcgag cattgtgaaa atcgatcatg agggcaatat
     6481 cgttgacgat gccaccgggc tggggatcaa ctacgccggc ttcgtcattc atggctgtgt
     6541 gcatgaggcc cgtccggaga tcaactgtgt gattcatacg cacacccgtg ccggagtcgc
     6601 ggtatcggtg cagaaccacg ggttgctgcc actcagccag catgcgctac gcacgtacgg
     6661 catgctgagc tatcacgact atgaaggcat cgcgctggag ctggacgaga gggcgcgaat
     6721 ggcggccgat ctcggaccta cttccaaggc catgatcctg cgcaatcacg ggttgctgac
     6781 gttgggcgat tctgtcggag aagcatttga actgatgtac taccttgaca ctgcgtgcca
     6841 aatccagatc gatgcactgg ccggtggacg ggtcgaagcg cgcctgatcg atgagaagac
     6901 cgcccgcaag gggttcgatc agttcaaggg accgggcggt gccgaggtca ataaggcctg
     6961 gcaggcactt ttgcgcatgc ttgaccgcaa gggcgtcaac tatagaaact agcaggtcgc
     7021 ggtcacgggc gacatatctg tcacgccatc gtaacggcgt gctccgagtt cagcgtaccg
     7081 tgccgggagt aacgaaccag gactctaccg gcaccggggt accgtgcggt gcgttatggt
     7141 ccgggttgaa ttccttgtat ttgctggcga gcatgtcgcg caccttatta tcccaatcgg
     7201 attgctttcc cgaatcgagc cgttcatagg ccttgcagaa ttcgcgagtg aaatagacgt
     7261 tcacgccctt ggctggcttg tgcggccgct tcgggtcggt gccgactttc catcgcactt
     7321 taaaccagac cgattcgcct gctggcgggt gcgtaatctg ggcgccattg ggaaatacca
     7381 tccgggcgat ttccacccat cgatcgactt gaaccgtgct catttgggga cttcattcgc
     7441 gcgcatggcg gataagaccg cgcagtattg ctcgggttcg acagttgccc tgacaggcgg
     7501 ccttctatac attcgcgtca ccgcgtgccg aaaaccatga aaaacgactc gctcctgtat
     7561 gaaatagcgc cggaagtgat gtggaccatc gtcatcgtat cggcggtgat ccttgccaac
     7621 atcaccatat ccgccgtatt gcgcggccgc ggctggcttt ccagggaaac caaactgcgc
     7681 gcgtcagtgt tctggcgcaa tttttcgctg ctagtcgctt tcatcgcgct cattttcatc
     7741 tggcgtgttg agctgcgtgc cgccgccctg tcgcttgccg cgttgagtgt cgcgctggtc
     7801 attgcaggca aggaactgtt tacgtccgtg ctgggatacg tccaccgcac cacgtccggg
     7861 agtttcactt ttggcgatgt catcgagatc aacaatatca aaggcgaagt tattgaccaa
     7921 acgctgttat ccacaaccgt gttggaaatg agcgaggagc acctgttcac cggcagagtg
     7981 gtccagtttc caaacagctt ctatgtctcc aacgcgatga agaattattc ccggctaggc
     8041 gattatcaac ttggcatggt gaccatccca ctggccggcg ggacggatgt ggaaacggcc
     8101 cgtcgcatcc tctccgaggt tgcaaccgcg gtgtgttcag agttcgtggc gcccgcgcat
     8161 gcggcactac gcgaattgga aggcgaacag tttatcgtga tgccgtcggc cgaacctcga
     8221 gtcagtatcc ggctgggcga tggcggaaag accacccttc tgctccggtt tccgtgtccc
     8281 gcgagtcagc gcacgcgcac ggagcaggag ttgctgacca gatacctggc cggcatgcgt
     8341 tcggcggcgg cgtcctgatc catacttggc tggcgcgatg agcgccgcgg cggcggcgcg
     8401 caaacaaatt atctgcgtag ggcacgccgc gcttgatgat gtgtttgccg tcgactcctg
     8461 gcccgctaac agcggcaaaa ttgcggcgca cggtttcacg cgaagcggtg gcggcatggc
     8521 ggccaatgct gcatcggcca tcgcacggct cggcggcgaa gccgtattct ggggacccac
     8581 gggcgaagac ccggttgcgg atgcgattcg ggaatcgttg attgacgagg gcgtcgacgt
     8641 gcacggcctg cggcgcttcg cgggcagaac gtcctctcat tcagcagtga tcgtcgacca
     8701 gcgcggggag cgcctggtcg tgggctatcg cggcagtgca ctggaggtgt ccgcagattg
     8761 gctgccgctc gagcagatac ggtgcgccgc ggcgttgctt gcggatgtgc gctggcccga
     8821 cggtgcgttg gtggcattgc atgctgctcg caatgccggc atcccggccg tcctggatgc
     8881 cgaaatagct ccggtgcaaa ccattgagct gcttgccgca gccgccagcc acgtcgtttt
     8941 ttccgagcgt ggcctggaag cctgttgtgg ccgcgatagc gctgcaggat tgcgcggcat
     9001 gctggaacac ggcgcgctca tggctgctgt gacgcgaggc gcagccggcg tcgactgggt
     9061 tgaagcgact ggccctggga aactcctgca ctgtccggcg tttccggtcg atacagtcga
     9121 tacgctcggc gcaggagatg tatttcacgg tgcctatacg ctggccctgg cagaaggccg
     9181 cctgatcggc gaggcgatcc gttttgcggc agcagccgcc gcaatcaaat gcagccggcc
     9241 cggaggccgt agtggaactc cgaaccgatc ggaagtcgaa acgtttttgg ctagcgccgg
     9301 tatgaaacca cgcgactaac cagcgatggt tacgccacca tccgcgacaa tggtttggcc
     9361 agtcacgaag gccgctgcgc gcgaggccag aaacaccgca atgccgccga tgtcatccgg
     9421 ctcgccgatg cgtcgcaacg gcgtctgcga ttcataacgc tggagtatct cggggttttc
     9481 ccacaaggcg cgtgcgaaat ctgtcttcac caaacccggt gcaatgcaat tgatgcggat
     9541 gttttgagga ccccattcca ttgcaaggtt gcgcgccagc tgcatgtcgg cggccttgga
     9601 aatggcgtag ccgccgagca ctgaggtgcc tttgagtccg ccaatcgagg aaacgatgat
     9661 gacaacgcca tcttttctcc gggccatttc gggcagcacc agattggcca gccagtgatt
     9721 ggagacgata ttgttctgca gaatcttgtt gaacgcatcg tctggcatgt ttgccatgga
     9781 cccgtagtac ggattgctgg ccgcattgca taccagcaca tcgattttcc cgcattggct
     9841 gcgcgtttga gtcacgagcg cttccagttg ggttttgtcg ccgatatttg caggaatcgc
     9901 gatggcgtcg ccccccgccg ctcggatgct gtttgcgact tcttcgcagg ccggtgcctt
     9961 ccggctcgag atcacgactt ttgcgcccgc tttggcaaga tgctctgcaa tggagcggcc
    10021 gatgccgcgc gtggagccgg taacgattgc tactttgccg tcaagtgcga atgcgtccat
    10081 aaagcttctc cgttgcaaaa aaatggccag ccgcggttgg cgatcgatgc cggaaatgcc
    10141 gcgcctactg ctggccttcc tcgattactt tcttcagatt ctcgaggccc atctcgtaga
    10201 ctttattcag tccgttgagt ggcgtggcat cgtcttgtcc gggtggtggc tcatcggtcc
    10261 agtaggcaac gcgcttgaag cgggctttcc agaccacctt gcttttgcca ccgccatcgt
    10321 cggaaaccgt gatcgtcggg aagtaatcgg tcgcccccac ttgaccaccg atgtaggtgt
    10381 accccaacga catagtccac ggttcatatg cgatcagctt ttcctgcacc ttcgttccat
    10441 tggcgcgggt gagttctcgg atagcaccga cctcgcggtt cttgccaaga atgagccggc
    10501 tttccacgat gaccggcagc cagcgcggca ggccattgaa gtcgctgacg acctgccaga
    10561 cggcttcagg cggtgcattg atcacgatgg aatgctcaat gctcatttct ggtgcttcgg
    10621 cagcagacgc gggtgcggcc agagccgcga tgatcagtga ggcaagtgcc tttaacgctt
    10681 tcatgtggtt ctccctgttg ttcagcatga ctatgcgaac tgttttttaa tgtcccggga
    10741 cgacggtttc gcatgacaac tcaaacctgt tcgcatccag cggggacaga gcctaccgca
    10801 ttaaacagag cgccgcgacc ccgttcgaaa tgacgaacaa ttgatttaga aagccgacag
    10861 aattgggccg gccttatcgg cgcgggagct tgtgctagca tcattctcct ttcactgacg
    10921 gaaacgatat gagtccggta ttgagcgccg aagaactcgc acactatcag caacgcggtt
    10981 acgttttgcc gcgctaccgg ctacccgatc cactgttgca aagcctgcgc aagagtctgg
    11041 accaactgct tgccgcttac accgatgttg cgcaggagga cctggccaat ccgcacatgc
    11101 ttccgcccac cagcggcccc gacatgaatc ccttcatggc ggcggcgcgc cacccggcca
    11161 tactggacat ggtggaacaa attattgggc cggacatcgt gttgtggatc ggccggattt
    11221 tgtgcaagcc gcccgtcaag ggcagggaag taccctggca tcaggatggc gaatactggc
    11281 cgatgcggcc gctggcaacg tgctcggtgt ggatcgccat cgatccggtt tcagtggcga
    11341 acggctgcat gcgcttcatc cccggctcgc atcagcgcaa ggagctgtac aaacaccatg
    11401 tcagcgatcg caaggacctg gtgctcaatc ttgaacttga ttcgaaccag tttgatgaac
    11461 gcgaagcggt caacgttgaa cttgagccgg gccagatatc gcttcatgac gtgcgcatga
    11521 ttcatggttc gcttgccaat acttcgggcc agcgtcgcgc ggctttgatc ctgcgctaca
    11581 tgccggggac ttcgcattac gaccggtcat tacgcgacca gcgccgcgac aaatcgccct
    11641 tcaatattta tgatcagccg ctatggctga tgcgtggcga agacaagtcg cgcaagaacg
    11701 atcttctgca cggacacgac atctggaaac gtcgttacgc cagcggacta ggcgtaagtt
    11761 ccggaagcgc gggatgaagg ggaccgcgcc aatcgtgacg gggtgacggt cgtaagacct
    11821 gaagccgcgc agttacgata gttaggctag ctacggtctc aaatgaacat cgccaatttc
    11881 ctggtgcgtt ccgcgcgcac gtttcccgat cgcccggcgg tatcgctcgg cgacacggta
    11941 tggataggct atggcgacct catgcggcga gtgtccgcga tggccgccgg attccgtaac
    12001 tcgcttgcac tggttccagg cgatcgggtc gcgcttgcga tggcaaattg tccgcaatac
    12061 atcgaaatct ggtatgcggc ctggtacgca ggactgacga ccgtaccgat gaattcaaag
    12121 ctgcatccaa aagagctcga attcatcctg cgcgattcgg gtgccaggat ctgcttcgct
    12181 tcttccgggc tcacttcggc agtagagggc ctgcgcggcg aactgccggc cctcgagcgc
    12241 gtacttgatg tgggatcgtc cgactatacg gcgctgtacc gggcgcaccc cggtatcgcc
    12301 atgcatcaga cggcccccga agggattgcg tggctgtttt acaccagcgg cactaccggc
    12361 cggccgaagg gcgtgatgat cacgcatcgc aacgttttgt ccatgtcact agcctatttt
    12421 gctgccgtcg ataccatcgg cccgcgtgat tgcgtgctgc atgccgcacc gatgtcgcac
    12481 ggctccggac agtacaattt tccgcatatg ctggtgggcg cgaatcaggt tattcctgat
    12541 agcggtcatt tcgatgctgc agagatattt gacttatgca ggtgctggac cggcgtgaca
    12601 atgttcgctg cgcccaccat ggtgcatcgc ctggttgcgc acgcaagcaa gcacgcgccc
    12661 gcattggatg gcctcaagac gatcgtgtac ggcggggggc cgatgtactt gcagggaatc
    12721 ctaaatgcgc tcgataccct ggggccgcgc ttcgcgcaaa tctacggcca gggcgaatca
    12781 cccatgacca ttacagcgct gtccaaagca cagatcacgg atcgcaatca accacgctat
    12841 gagcagcggc tgg
//
LOCUS       DATMZQ010000421         2766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1551834, whole genome shotgun sequence.
ACCESSION   DATMZQ010000421 DATMZQ010000000
VERSION     DATMZQ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551834"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VN496_13405"
     CDS             complement(<1..399)
                     /locus_tag="VN496_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF17999.1"
                     /translation="MISAGIASFNRHGVLAIYRFELSRFKRTLWQSLVTPVITTSLYF
                     VVFGAAIGARMNEVSGVHYGAFIVPGLIMLSLFTESLNNASFGIYLPKFTGTIYEILS
                     APVSAVEIVLAYVGAAATKSVILGLVILATA"
     gene            complement(396..1322)
                     /locus_tag="VN496_13410"
     CDS             complement(396..1322)
                     /locus_tag="VN496_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18000.1"
                     /translation="MAPVISVAKLTKKYASGLQALNNIDLEIRGGEIFALLGPNGAGK
                     TTLINIICGLVTPTSGTVLTDGHDIVREYRASRAKIGLVPQELHTDTFETVWNTVRFS
                     RGLFGRAPDAVHLEKVLRDLSLWEKRNAKIMTLSGGMKRRLLIAKALAHEPEILFLDE
                     PTAGVDVELRRDMWRLVRRLRDSGVTIILTTHYIDEAEEMADRIGVINKGELILVEEK
                     TELMKKLGKKRLTLQLQEPMAAVPAGLAQWRVTLQDGGNALVYDFDARDEGTGIPMLL
                     RRVSELGIAFKDLQTQQSSLEDIFVSLVHQRR"
     gene            complement(1441..2019)
                     /locus_tag="VN496_13415"
     CDS             complement(1441..2019)
                     /locus_tag="VN496_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXF18001.1"
                     /translation="MPFRKTILVAVLAGAIAPGVATADGYTIDPQHTYPSIAFSHMGI
                     SVWRGKFNKTHGSIMLDRAAKSGSADIVIDVASINFGLDAMDEKARSDDFFDVSRFPT
                     ATYKGAIKFNGDKPASVDGKITIRGITRPVVLHIELFNCIVHPMLKREVCGAEAQGEL
                     NWSEYGMKMSQYGQGDAGKVQLRIQAEAIKND"
     gene            complement(2065..2478)
                     /locus_tag="VN496_13420"
     CDS             complement(2065..2478)
                     /locus_tag="VN496_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF18002.1"
                     /translation="MLIQTYLSFDGRCEEAIEFYRRKVGAEVTMLMRYKEAPEPAPPG
                     MLPPGAENKIMHAALRIGDTTIMASDGNCKGKPNFQGFSLSLSVPNEVEAERLFAALG
                     EGGQVQMPMSKTFFAPRFGMVADRFGVSWMVIVAT"
     gene            complement(2574..>2766)
                     /locus_tag="VN496_13425"
     CDS             complement(2574..>2766)
                     /locus_tag="VN496_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18003.1"
                     /translation="FNPHTRHAWDAGAAVMVAAPSCIVADALTKVAALAGPACAPLLA
                     RFGAHARWDTELHRDVVVN"
BASE COUNT          547 a          830 c          771 g          618 t
ORIGIN      
        1 ggcagtggcc aggatcacca gcccgagaat gaccgacttg gtggcggccg cgccgacgta
       61 ggcaagcaca atctcgaccg ccgatacggg cgctgaaagg atctcgtata tcgtgccggt
      121 aaatttgggc aggtatatac cgaacgaagc attattcagg ctttcggtaa acagtgacag
      181 catgatcagg cccggcacga tgaatgcgcc atagtgcacg ccgctgactt cattcatgcg
      241 cgcgccaatg gcagccccga acaccacgaa atagagcgaa gtcgttatta ccggcgtcac
      301 cagactttgc catagcgtgc gtttgaagcg cgacagctca aagcggtaaa tggccagaac
      361 gccatgacgg ttgaatgagg caatgccggc gctaatcatc tgcgctgatg gaccaggctc
      421 acgaaaatat cttccagtga gctttgttgg gtctgcagat ccttgaacgc aatgccaagc
      481 tcgctcaccc ggcgtagcag catcgggatg ccggttcctt cgtcacgagc atcgaagtcg
      541 tagactaatg cattaccgcc gtcttgcaga gtcacccgcc actgcgccag tcccgcagga
      601 acggccgcca tcggttcctg caactgcaag gtaagccgct tcttgccgag ctttttcatg
      661 agctcggttt tttcttcgac cagaatcaac tcacctttgt tgatgactcc gatgcggtcc
      721 gccatttctt cggcctcatc gatgtaatgc gtggtcagga tgatggttac gccgctgtca
      781 cgtagtcgcc gcaccaggcg ccacatatcg cggcgcaatt caacatcgac accggctgtc
      841 ggttcatcga gaaaaaggat ctcgggctcg tgcgccagcg ccttcgcgat cagcaaacgg
      901 cgcttcatgc caccggacaa ggtcatgatt ttggcgttgc gcttttccca taacgacagg
      961 tcccgcagca ccttttcaag atgcactgca tccggcgcgc gcccgaaaag tccgcggctg
     1021 aagcggaccg tgttccatac cgtctcaaag gtatcggtgt gcaattcctg gggcaccagg
     1081 ccgattttcg cgcgcgacgc ccggtactcc cgcacgatgt catggccatc agtcagcact
     1141 gttccactcg tcggcgtcac caggccgcag attatgttga tgagcgtggt cttgccggcg
     1201 ccattcgggc cgagcaaagc gaatatctct ccgccgcgaa tttcaagatc gatattgttc
     1261 aacgcctgca ggccggacgc gtatttcttc gtcagcttcg cgacgctgat aacgggcgcc
     1321 atacgggaga ccgcgctggc ttgcctgcct ttggattgcg cgcttgtttc tactagatcc
     1381 atgattagcc ctgatcactg gcgcaacatg ttggccgttg tcctgcgttc acccggcaat
     1441 tcagtcattc ttgattgctt cggcctgaat gcgtaactga accttgccgg catcgccttg
     1501 cccatactgg ctcatcttca tgccgtattc ggaccagttg agttcgcctt gcgcttcggc
     1561 cccgcagacc tccctcttca gcatgggatg gacaatgcag ttgaacagtt caatgtgcaa
     1621 tacgaccggg cgcgtgatgc cccggatggt tatctttcca tcgacgctcg ccggcttatc
     1681 gccgttgaat ttgattgcac ccttgtaggt cgcggtggga aaccggctga catcgaaaaa
     1741 atcatccgag cgtgcttttt cgtccatcgc gtccaggccg aaattgatgc tcgccacatc
     1801 gattacgatg tccgcgctgc cgctcttcgc tgcccggtcg agcatgatgc tgccgtgcgt
     1861 cttgttgaat ttgccgcgcc agaccgaaat tcccatgtgc gagaacgcaa tgctcggata
     1921 cgtgtgctgc ggatcgatgg tgtagccgtc ggccgttgca acgccagggg ctattgcacc
     1981 ggccagtact gcaacaagga tggtctttct gaatggcatg agttgtcctc cgtggtttgg
     2041 ggtattgctg aatcgccccg gcgctcaggt cgctacgatg accatccatg acacgccaaa
     2101 gcggtcggcg accatgccga aacgtggcgc aaaaaaagtc ttgctcatgg gcatctggac
     2161 ctggcctccc tctcccaacg cggcgaacag tcgttcagct tcgacttcat tcggcaccga
     2221 cagcgacagc gaaaacccct ggaaatttgg cttgcctttg cagttgccat cggaagccat
     2281 gatcgtcgtg tcgccgatgc gcagtgccgc gtgcatgatc ttgttttctg cccctggcgg
     2341 cagcatgcct ggcggtgcgg gctcgggtgc ctccttgtag cgcatcagca tcgtaacttc
     2401 agcgccaact ttgcgccggt agaattcgat cgcctcctcg caacggccgt cgaaagacag
     2461 atacgtttga atcagcatgg tgtcttgccc tctgatttga ttgaacggct gccgccgcgg
     2521 tgaatccagt gcctttgttt ggtggcagat ccagcatctg gttcccgacc cgcctaattt
     2581 acaactacat cacgatgcaa ctcggtgtcc caccgggcgt gggcgccgaa acgggccagc
     2641 agtggcgcgc atgcggggcc cgcaagggca gcgaccttcg tcaatgcatc agccacgatg
     2701 caactcggcg cagcaaccat cacggcggcg ccggcgtccc aggcgtggcg cgtgtgggga
     2761 ttgaag
//
LOCUS       DATMZQ010000422         4364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3101236, whole genome shotgun sequence.
ACCESSION   DATMZQ010000422 DATMZQ010000000
VERSION     DATMZQ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101236"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VN496_13430"
     CDS             complement(<1..493)
                     /locus_tag="VN496_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF18004.1"
                     /translation="MPKKPPQLAKLTRPRLYKAVARERLFHLLDQKREHPVVWIVGPP
                     GAGKTTLAASYLEEAGVPAIWYQVDPGDSDPATFFYYLKQGVERLAIPKAKSLRLLTS
                     EYLSDVPGFARRFIRTVFSQLPEDALLVFDNYQEIASDSSLHVAFSSALTEIPPGGNI
                     LVLS"
     gene            complement(620..1774)
                     /locus_tag="VN496_13435"
     CDS             complement(620..1774)
                     /locus_tag="VN496_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HXF18005.1"
                     /translation="MAAKPTPFSLHVGDDVLDDLKTRLARVRWPDEVPDNHWKYGTDL
                     PYLKALVAYWRDQYDWRKHEAQLNRFTQFKVKLGGIDLHFIREEGKGRNPMPLLLSHG
                     WPGSVHEFHKIIPMLTDPARFGGDPADAFTVIAPSLPGYTLSFTPNQQRFSIEQIADV
                     FAELMTDVLGYQRFAAQGGDWGGFVTSRLGYAYPERLAGIHINLLSLRRDTAPPQRPS
                     AEEQRYFAERAHWMKEEVGYTWIQGTKPQTLAYGLSDSPVGLAAWIVEKFHTWSDHRG
                     DLDAYLGRDDLLTNIMLYWITGAIGSSFWPYYARMHGPWPIPDGARIGVPMGYAEFPK
                     EILRPPRSLAEKMYSDIRRWSKMPKGGHFAALEQPELLAQEIREFFRPLR"
     gene            complement(1911..2492)
                     /locus_tag="VN496_13440"
     CDS             complement(1911..2492)
                     /locus_tag="VN496_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18006.1"
                     /translation="MAAALGSLALLFSANIVAEEISPPAVPKPGIQEGMYSTYADDRS
                     VISQSSIGDASAATLSAAELMPALVSALDQLSKYHRLVALPEIIQVSHQRIEEIVCHA
                     KCGALAAYRPGEGIYIDERLKPETDLFARSILLHELVHYAQEMSGEHGDMRPCERWYH
                     REQEAYAIQKKFLFIAGSPTRVGYSAAKSTCDD"
     gene            complement(2563..3420)
                     /locus_tag="VN496_13445"
     CDS             complement(2563..3420)
                     /locus_tag="VN496_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF18007.1"
                     /translation="MPGMRQQTIRANGIRVNTWVGGEGDPVVLLHGYPQTAQMWRKVA
                     PVLLNRFTVVCPDLRGYGDSDKPRDGYDKRSMAKDVVETMKAFGFARFALVGHDRGAR
                     VSHRLAMDYPEAVKNLTVLDIVPTHTVFRDAGKDLAAAYWHWFFFQLPDLPEIMIANS
                     AEAFLRNMFRALCFRQDAIEEPMFAEYLRAFTLPGTIRGGLEDYRAAATVDIADDERD
                     LGKKLSCPVLAIWGEQGKMHTLFDVLKTWREKADDVRGHALMCGHFIPEEAPEDLLRD
                     LVPFLSHGR"
     gene            complement(3559..>4364)
                     /locus_tag="VN496_13450"
     CDS             complement(3559..>4364)
                     /locus_tag="VN496_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXF18008.1"
                     /translation="MADPGYPCNRHFVRTMEGEPIGIPVGADTAYQLTANLVEKHWSP
                     KTAAVLIASPSNPTGTLIDHGELQRIHAIVKAHGGVLIVDEIYHGLTYGKRDRSALEF
                     ADDIFIINSFSKYFGMTGWRIGWMVAPEQYIEPVDKLAQNLFISPSDLAQQAALAAFH
                     PDTVALLEQRRDAFRAQRDFLLPQLRSLGFDIPVTPEGAFYIYANCERHTSDSYQFCW
                     DLLEKAGVAIAPGLDFGDHRAPQHVRFSYPKSIPVLQDGVERLRKYLGK"
BASE COUNT          943 a         1259 c         1247 g          915 t
ORIGIN      
        1 ggcttagtac caatatgttt ccgccaggag gtatttccgt gagcgcggat gaaaatgcca
       61 catgcaaact tgagtctgat gcaatttcct gatagttatc gaagacgagc aacgcatcct
      121 ctgggagctg tgaaaaaacc gtcctaatga atctgcgtgc aaaacctggc acatcagaca
      181 aatattccga agtaagcagc cggagagatt ttgcttttgg tattgcaagg cgttcgacgc
      241 cttgcttcaa ataatagaaa aaagtggccg gatcgctatc accgggatcc acctgatacc
      301 aaattgcagg cacaccggct tcttccagat aactcgccgc aagcgtcgtc ttgcccgctc
      361 ctggtggccc gacaatccag accaccgggt gttcgcgttt ctgatcgagc agatgaaaga
      421 gtctttcccg ggcgacggct ttataaaggc gaggtcgggt gagcttggcg agctgtggcg
      481 gctttttcgg cattcccgct ccagcagaaa aaacacgatg acagcgccat tgttgcgcga
      541 tccgccgcaa aagaaaacgc ccggtttgtc agctgagcga cagctctcag cgagtcttat
      601 cccagccttt gccgaaggct cacctcagcg gccgaaaaaa ctcccgaatc tcctgcgcca
      661 gcaattccgg ctgctccagc gccgcaaagt ggccgccctt cggcatcttc gaccagcggc
      721 ggatgtcgct gtacatcttt tccgccagcg atcggggcgg gcgcaggatc tccttcggaa
      781 actccgcgta gcccatgggt acgccgatgc gggcgccatc ggggatgggc cacggcccgt
      841 gcatgcgagc gtaatacggc cagaacgaag agccgatcgc gccggttatc cagtagagca
      901 tgatgttcgt caacaggtcg tcgcggccga gataagcatc gaggtcgccg cggtgatcgc
      961 tccacgtatg aaacttttca acaatccacg cagccagccc taccggcgaa tcgctcaacc
     1021 catacgccag cgtctgtggc ttggtgccct gaatccaggt atagccgact tcttctttca
     1081 tccagtgcgc gcgctctgca aaatagcgtt gttcttctgc gctgggacgt tggggaggtg
     1141 ccgtatcgcg ccgaaggctc aacaaattga tatggatgcc ggccagtcgc tcgggatacg
     1201 cgtatccaag cctcgatgtg acgaacccgc cccagtcgcc gccttgagcc gcgaagcgct
     1261 ggtaacccag cacgtcagtc atgagttcgg caaacacatc agcaatctgc tcgatggaga
     1321 agcgttgctg attgggtgtg aaagacaacg tatagccggg caaagatggc gcaatgacgg
     1381 tgaaggcatc cgcgggatcg ccgccgaaac gcgccggatc ggtcagcatc ggaatgatct
     1441 tatggaattc gtgcaccgag cccggccagc cgtgcgagag cagcagtggc atgggattgc
     1501 gtcccttgcc ttcttcgcga atgaagtgca aatcgatgcc gcctagcttg accttgaact
     1561 gagtgaatcg gttcagctgc gcttcatgct tgcgccagtc gtactgatca cgccagtacg
     1621 cgacgagcgc tttcagatac ggcaggtcgg tgccgtactt ccaatggttg tcgggcacct
     1681 cgtctggcca acgtactcgc gccagtcgcg ttttgagatc gtcgagcaca tcgtcgccga
     1741 catgcaagga aaagggcgtt ggtttggcgg ccatgtcgtt ctccagggaa gccgcattgt
     1801 tcgctactcg cgcgacagcg tccaatatat tgccgaaggc gcactgatag ccgccgtgaa
     1861 tcaggcttta cgctttagag gaacggcacc ctcggacagc gattgatcgc tcaatcgtcg
     1921 caggtggatt tggctgcgga gtacccgacg cgcgtggggc tgccggcaat gaacaggaat
     1981 tttttctgga tagcataggc ctcctgctcg cggtggtacc agcgctcgca gggcctcatg
     2041 tcgccgtgct ccccgctcat ctcttgcgcg tagtgcacga gctcatgcaa caggatcgag
     2101 cgggcgaaca agtctgtttc cggtttgagg cgttcatcga tatagatgcc ttctccgggg
     2161 cggtacgcag caagagcgcc acacttggcg tggcacacta tttcttcgat acgctggtgc
     2221 gaaacctgga tgatttcggg caatgccacc agccggtggt atttggatag ctggtccagc
     2281 gcggacacca gggccggcat cagctctgcc gccgaaagtg ttgccgcgct cgcgtctcca
     2341 atggaagatt gggatatgac gctgcgatca tccgcatagg tactgtacat tccctcctgg
     2401 atgcccggct tcggcacggc gggcgggctg atttcttccg caacgatatt ggccgaaaac
     2461 agcagcgcaa ggctgcccaa tgctgccgcc atcagattca agcgggacgc tggccggggc
     2521 tgccaatgtt tgccccgcat gcaagacaat ggatccattg tgtcagcgac cgtggctcag
     2581 gaaagggacc agatcgcgca acaaatcctc gggcgcttct tccggaatga aatggccgca
     2641 catcaacgcg tgcccgcgca catcgtcagc tttttcacgc catgtcttga gcacatcgaa
     2701 taacgtatgc attttgcctt gctcccccca tatggccagt accgggcagg aaagtttctt
     2761 cccgaggtcg cgttcgtcgt cggcaatgtc aaccgtggcg gcggcacgat agtcctccag
     2821 gccgccgcgt atcgttcccg gcaaggtaaa ggctcgcagg tattccgcaa acatcggctc
     2881 ttcgatcgca tcctgccgaa aacaaagggc acggaacatg ttgcgtagaa acgcctctgc
     2941 gctgttggcg atcatgattt ccggcaggtc cggcagctga aagaagaacc agtgccagta
     3001 agcggccgcg agatcttttc cagcatcgcg aaataccgta tgggtcggta cgatatccag
     3061 cacggtcaga tttttgacgg cttcaggata atccatggcc aaacgatgtg aaacgcgcgc
     3121 gccacgatcg tgcccgacca gcgcgaaacg cgcaaagccg aacgccttca tggtttccac
     3181 gacgtccttc gccatgctgc gcttgtcgta gccatcgcgc ggtttatcgg aatcgccata
     3241 gccgcgcaaa tcgggacata ccacggtgaa gcggttcagc agcaccggcg cgaccttgcg
     3301 ccacatctgc gccgtttgtg gataaccatg cagcaacaca acgggatcgc cttcgccgcc
     3361 gacccacgtg ttgacgcgga ttccgttggc gcggatggtc tgttgcctca tgccaggcat
     3421 gaagttcata agagtccgtg acgatcgtaa cgacgtgacg acgaaacgac gtaaggttgt
     3481 ggctttgcgc ttgtcataga gttagctctt tgtatgcttg taaaagcgtt accgcttccc
     3541 tcaacgtcgt cacgggcatt attttccaag gtacttgcgc aaccgctcga ctccatcctg
     3601 caacaccgga atcgattttg gataggaaaa gcgcacatgc tgcggggcgc gatggtcgcc
     3661 gaaatccaat cctggagcaa tggcgacgcc ggccttttca agcaggtccc aacagaactg
     3721 atagctatcg gatgtgtgcc gctcacaatt tgcatagata tagaaggcac cttccggcgt
     3781 aaccggtatg tcgaagccta gactgcgcaa ctgcggcaac aggaaatcgc gctgagcgcg
     3841 aaacgcgtcg cgcctttgct ccagcagcgc aacggtgtcg ggatgaaacg cggcgagtgc
     3901 ggcctgctgt gccaggtcgg aaggagaaat gaacagattc tgcgccagtt tgtcgaccgg
     3961 ctcgatgtac tgttccggcg cgaccatcca gccgatgcgc caccccgtca tgccgaaata
     4021 tttggagaaa ctattgatga taaaaatatc gtcggcaaat tccagtgcgc tccggtcgcg
     4081 cttgccataa gtcaggccat ggtaaatctc atcgacaatc agcacgccgc catgcgcctt
     4141 gacgatggca tggatacgct gcagttcgcc gtgatcgatc aacgtgccgg tcggattcga
     4201 cggcgatgca atcagtaccg cggcggtttt tggactccag tgtttttcca cgagattcgc
     4261 agtgagctga tacgccgtat ccgcgccaac tggaatgccg atgggctcgc cttccatggt
     4321 gcgcacgaaa tggcgattgc acgggtagcc cggatcggcc atca
//
LOCUS       DATMZQ010000423         6718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2241401, whole genome shotgun sequence.
ACCESSION   DATMZQ010000423 DATMZQ010000000
VERSION     DATMZQ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241401"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /locus_tag="VN496_13455"
     CDS             <1..2068
                     /locus_tag="VN496_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HXF18009.1"
                     /translation="EIISACLAILERPEISLDELIELVPGPDFPTKGIIYGTAGVKEG
                     YRTGRGRIVIRARTHVEELEKGNRSAIVIDELPYQVNKSSLLMKIGEMVREKRLDGLS
                     EIRDESDKSGMRVVLELKRGEVPEVILNNLYKETQMQDTFGMNMVALVENQPRLLNLK
                     EILDAFLRHRREVITRRTVFELRKARDRGHVLEGLAVALSNVDEIIALIKAAAAPAEA
                     KRELMTRTWRSQLVESMLARAGVQEARPEGISAEFGLQPDGYRLSEVQSQRILEMQLQ
                     RLTGLEQEKIVNEYREVMDTIADLLDILARPERITVIIRDELSKTREEFGDERRSEIV
                     MNAQDLSMEDLIAPEDVVVTLSHSGYVKAQPLADYRAQKRGGRGRQATATKENDFIDN
                     LFIANTHDYVLCFSSHGQLYWIKVYNVPQGGPASRGKPIVNLLPLTEGEKITAILPVR
                     EFDENKYIFMATANGTVKKTPLSDFSRPRPSGIIAVDLDEGDYLIGVALTEGRHDIML
                     FSDAGKAVRFDEQDVRPMGRVSRGVRGMRLSGGQKVISLLVAENESVSVLTATENGFG
                     KRTPITEYTRHGRGSQGMIAIQTTARNGNVVAATLVDNSDDIMLITTGGVLIRMRVAE
                     IREMSRATQGVTLINLGPEEKLAGLQKIVDSEDLGEALDAVAEPVDEDTGPGAEDHQS
                     GPDSTE"
     gene            2119..3201
                     /gene="serC"
                     /locus_tag="VN496_13460"
     CDS             2119..3201
                     /gene="serC"
                     /locus_tag="VN496_13460"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HXF18010.1"
                     /translation="MARVFNFSAGPAVLPQPVLEKARDEMLDWHGSGMCVMEMSHRGK
                     EFISIHAHAEATLRELLAVPNNYKVLFLQGGAIAENAIVPMNLLRGLDVADYINTGEW
                     SKKSIKEAKKYCNVNVAASSEDKNFSYIPHRETWRLSKNAAYVHICSNETIGGVEYHW
                     VPETGEIPLVADMSSNILSRPVDVSAYGLIYAGAQKNIGPAGLTIVIVRDDLIGQASP
                     FTPSAFDYRQQAENDSMLNTPPTYAIYIAGLVFDWLKQQGGLAQVEKHNMAKAALLYN
                     YLDASGLFVSPVDKRDRSRMNVPFKLRQESLDEQFLKGAQQRGMLQLKGHRSVGGMRA
                     SIYNAMPIEGVKTLVGYMEEFEKSHG"
     gene            3194..4375
                     /locus_tag="VN496_13465"
     CDS             3194..4375
                     /locus_tag="VN496_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXF18011.1"
                     /translation="MAELMSILTLNAISPSGLQRFPSERYSVGAQVQTPAAILVRSQD
                     MRTMRIAESVHAIGRAGAGTNNIPVQEMSKRGIPVFNAPGANANAVKELVLAGMLIAA
                     RNLVPAIRFTDGLSGSDEEIEKKVEDGKKNFAGVELPQHVLGVIGLGKVGSLVADAAI
                     KLGMEVFGYDPEITVDAAWSLPSQVRKAHSIEEVLRSSDFVTLHVPLLDGTRNLIDAK
                     RIGLMKSAAVLLNFSRDGIVDNTAVLDALDAAKLKAYVCDFPGARLKGHPAVIATPHL
                     GASTQEAEENCAIMVVDQVRDYLENGNIRNAVNFPDVVMSRESPYRLGISNANVPNMV
                     GQISTTLAQAGLNIHNMLNKSKKDMAYTLVDVDSAVQRNVIEKIAAIAGVLAVRYLPA
                     A"
     gene            4380..5447
                     /gene="pheA"
                     /locus_tag="VN496_13470"
     CDS             4380..5447
                     /gene="pheA"
                     /locus_tag="VN496_13470"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXF18012.1"
                     /translation="MADKLRQSRERIDALDLEILKLVNERASTAHAIGKLKDDGIVYR
                     PEREAQVLRRLAEANLGPLSGKVVMHLFTEIISACRALEDAFSVASLGPKGTFSEEAV
                     VKHFGGQSPVVLCASIDEVFRSVETGSVGYGVVPIENSTEGAIGRTLDLLLSTQLRLC
                     GEVLLPVHQNLLSKGTDIAAVTRIFSHAQSLAQCNRWLAQHCPRAERVPVTSNAEAAK
                     MAQEADFSAAIAGRSAAAHYGLAVLADNIEDEPNNTTRFVVLSAQEPAPSGNDKTSLV
                     MSTRNVPGAIHELLTPLANNGVSMTRLESRPSRTGLWEYVFFVDMEGHQNEEHVARAL
                     KEMRELAAFLKILGSYPIAVQ"
     gene            5444..6568
                     /gene="hisC"
                     /locus_tag="VN496_13475"
     CDS             5444..6568
                     /gene="hisC"
                     /locus_tag="VN496_13475"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXF18013.1"
                     /translation="MSPVTAEQLVDVCERAPAYVRAIAPYQPGKPISELAREMGLQES
                     SIVKLASNENPRGASPKAIAAMRIAIGATGRYPDGNAYALKDALTKRYGVAHDRIVLG
                     NGSNDVLEMAARAFLSPKASAVYSEHAFVVYSLATQAVGATGIVVPAHNFGHDLDAMS
                     KAIRSDTRVVFIANPNNPTGTWLPPEQIHGFLTKTPADVLVVLDEAYNEYLDPDLRSN
                     AVAWSEEFPNLVVSRTFSKAYGLAGLRVGYGFSHPGVIDLMNRVRQPFNVSDIGQVGA
                     AAALFDDEFVQQSTLLNRHGMKALTEAFTSLGLSWIPSHGNFVCVKVGKAAEVFQRLL
                     KQGVIVRPIVSYGMPEHLRVSIGTESENARFLAALAVSIQ"
BASE COUNT         1547 a         1766 c         1986 g         1419 t
ORIGIN      
        1 cgagattata agcgcctgcc tcgccattct ggagcgtccg gaaataagtc tggacgaact
       61 catagaactc gtgcccggcc cggacttccc caccaaggga atcatctatg gcaccgctgg
      121 cgtaaaagag ggttatcgca cgggacgcgg ccgtatcgtt atccgcgccc gcacacatgt
      181 ggaagaactg gagaagggca atcgttcggc cattgttatc gacgaattgc cttatcaggt
      241 caacaaatca tctttgttga tgaagatcgg cgagatggtg cgggaaaagc gcctggatgg
      301 cttatctgaa attcgcgatg agtcggataa atccggtatg cgagtagtgc tggaactgaa
      361 acgcggcgag gtccccgaag tcatcctgaa taatctgtat aaagaaacgc agatgcagga
      421 taccttcggc atgaatatgg tggcactggt tgaaaaccag cctcgcctgc ttaacctgaa
      481 ggaaatactg gacgcttttc tgcgccaccg tcgcgaggtc attacgcgcc gcacggtatt
      541 cgaactcaga aaggcgcgtg accgcggtca tgtgctcgaa ggcctggcag tcgcgctatc
      601 gaacgtcgac gaaattattg cgctcatcaa ggcggcggcc gcgccagcag aagcaaaacg
      661 agaattgatg acgcgcacgt ggcgttccca gcttgtcgaa tcgatgcttg cgcgtgcggg
      721 cgtacaggaa gcgcgtccgg aagggatttc cgcagaattt ggtttgcagc cggacggata
      781 ccggctctcc gaagtacagt cacagcgcat tctcgaaatg cagctccagc gtctcacggg
      841 gctggaacag gaaaaaatcg tcaacgaata ccgcgaggtc atggatacca tcgcagactt
      901 gctggatatc ctggccaggc cggagcgtat taccgtgatt atcagggacg aactctccaa
      961 gacccgggaa gagttcggtg atgaacggcg tagcgaaata gtcatgaatg cacaggacct
     1021 gagtatggag gacctgattg cgccggaaga tgtcgtagtg acgctttccc atagcggata
     1081 tgtcaaggcg cagccgctcg ctgattatcg cgcgcagaag cgcggcggcc ggggcaggca
     1141 ggctacggct accaaggaaa acgatttcat tgacaacctg ttcattgcca atacgcacga
     1201 ttatgttctt tgtttctcca gccacgggca gctttactgg atcaaggtct ataacgtccc
     1261 gcagggcggt ccggccagcc gcggcaagcc gattgtcaat ctgcttccgc taacggaagg
     1321 cgaaaagatc acggccatac tcccggtgcg cgaattcgac gagaacaaat acattttcat
     1381 ggccacggcg aatggcacag tgaaaaagac gccgttgtca gatttttccc gtccgcggcc
     1441 ctccggcatc attgcagtag accttgacga gggcgattac ctgatcggag ttgccctcac
     1501 cgagggccgc cacgacatca tgttgttctc agacgcaggc aaggcggttc gtttcgatga
     1561 gcaggatgtg cggcccatgg gacgcgtatc gcgcggcgtg cgcgggatga gactttcggg
     1621 cggacagaag gtgatatcgc tcctggtcgc tgaaaatgag tcggtttcgg ttttgaccgc
     1681 gacggagaac ggatttggga agcgtacccc catcacggag tacacgcgac acggccgggg
     1741 gtcgcaaggc atgatcgcga ttcagaccac cgcccgtaat ggcaacgtcg tcgcggctac
     1801 gctggtggat aacagtgacg acatcatgct gattaccact ggcggtgtgc tgattcggat
     1861 gcgggtcgcg gagattcgtg aaatgagtcg cgcaacgcag ggcgttacat tgatcaatct
     1921 tgggcctgaa gagaaactgg ccggattgca aaaaatcgtc gatagcgaag atctcgggga
     1981 agctttggat gcggttgccg agcctgtcga tgaagataca ggcccgggtg ctgaagatca
     2041 tcagtccggc ccggatagta ccgaataaca atatcgatgc cgcgtgcatt gacgcggatt
     2101 tcagtaagcg gagcaacggt ggcaagagta ttcaatttca gcgcgggacc cgcggttttg
     2161 ccgcaacctg tattggaaaa agcgcgtgac gaaatgctcg attggcacgg atcgggtatg
     2221 tgcgtgatgg agatgagtca tcgcggcaaa gaatttattt ctatccacgc ccacgccgaa
     2281 gccaccttgc gcgagctgct ggctgttcca aacaattaca aggtgctatt cctgcagggc
     2341 ggagcgattg ccgaaaacgc cattgtgccc atgaaccttt tgcgtggtct tgacgtcgcg
     2401 gactatatca atacaggtga gtggtccaag aaatctatca aggaggcaaa aaaatattgc
     2461 aatgtgaacg tggctgcgtc gagcgaagat aaaaattttt catatatccc gcaccgcgaa
     2521 acatggcggc tcagcaagaa tgccgcctat gtccacatat gctcaaacga gacaatcgga
     2581 ggggtggaat atcactgggt tccggaaacc ggcgaaattc cgctcgtggc cgacatgtcc
     2641 tcaaacatat tgtctcgacc ggtagatgtt tccgcctacg gattaattta cgctggggca
     2701 cagaagaaca ttggcccggc aggcctgacg atagtcatcg tccgtgatga tttgatcggc
     2761 caggcatcac ccttcacgcc ttcggctttt gattaccgac aacaagctga aaatgactcg
     2821 atgctgaata cgccgcccac ttatgcgatc tatatcgcag ggctggtatt cgattggctc
     2881 aaacagcagg gtggtctggc gcaggtcgaa aagcacaaca tggcgaaggc ggcactgctg
     2941 tacaactatc tggacgcgag cggtttgttc gtcagtccgg tcgacaagcg ggatcgctcg
     3001 cgcatgaatg tgccattcaa gcttcgtcaa gaatcgctgg acgagcaatt tttgaagggc
     3061 gcccagcaac gcgggatgct gcagctcaag ggccatcggt cggtcggcgg gatgcgcgcc
     3121 tccatttaca atgccatgcc aatcgaaggc gtcaagactc tggtcggata tatggaagaa
     3181 tttgagaaaa gccatggctg aactgatgtc cattcttacc ctgaatgcca tttcaccgtc
     3241 cggcctgcaa cgtttcccgt ccgagcgcta ttcggtgggc gctcaagtcc agacgccggc
     3301 ggctatcctg gtgcgctcgc aggatatgag aactatgcgg attgccgagt cggtacacgc
     3361 gattggcagg gccggtgccg gcacgaacaa tatcccggtg caggaaatga gcaagcgcgg
     3421 aatcccggta ttcaatgcac cgggcgctaa tgccaacgcg gtgaaggagc tggttttggc
     3481 cggtatgctg attgctgcgc gcaatctcgt tccggccatc cgttttacgg acggattgtc
     3541 aggcagcgac gaagagattg agaaaaaagt cgaagacggg aagaagaatt tcgcaggcgt
     3601 cgaacttcca cagcatgttc ttggcgtcat cggactcggt aaggtaggca gcctggtcgc
     3661 ggatgcggcg atcaagcttg gtatggaagt gttcggttat gaccccgaga ttacggtgga
     3721 tgcggcctgg agtcttcctt cacaggtcag aaaagcacac agcattgaag aagtgctccg
     3781 cagcagtgat ttcgtgaccc tgcacgtgcc cctgctggat ggaacgcgca acctgataga
     3841 cgccaagcgc atcgggctga tgaaatcggc ggcagtgctg ctgaattttt cgcgcgatgg
     3901 cattgtcgac aatactgcag tgctcgatgc actcgatgct gcaaagctca aagcctatgt
     3961 gtgcgatttt cccggcgccc gcctgaaggg acatccggca gtaattgcga caccgcacct
     4021 gggagcctca actcaggaag cggaggagaa ttgcgcaatc atggtcgttg accaggtgcg
     4081 agactatctg gaaaacggca atattcgcaa tgcagttaat ttcccggatg tggtcatgtc
     4141 ccgggaatca ccgtatcggc tcggaatttc caatgcgaat gtgcccaata tggtgggcca
     4201 gatttcaacg acgctcgcgc aggccgggct gaatatccat aacatgctga ataagtcaaa
     4261 aaaggacatg gcttacacgc tggtagacgt cgatagcgcc gtgcagcgca atgtcataga
     4321 aaaaattgcc gcgatcgcag gcgtgctggc ggtccgttac cttccagcgg cctaagtaaa
     4381 tggccgataa gctgaggcag tcccgcgagc gtatcgatgc cctggacctg gaaatcctga
     4441 agctcgtgaa tgagcgagct tcgacggccc atgctatcgg caagctaaag gacgacggta
     4501 tcgtctaccg accggaacgc gaggcgcaag tgcttcggcg gcttgcggag gcgaatcttg
     4561 gcccgttgtc gggcaaggtc gtcatgcacc tgtttacgga aatcatatcc gcgtgtcgtg
     4621 cgctggaaga tgcgttctcg gtggcgagcc tgggaccgaa gggcacattc agcgaagaag
     4681 cggtcgtcaa gcattttggt ggccaatccc cggtcgttct gtgcgcctcg attgacgaag
     4741 tattccgttc cgtggaaacg ggaagcgtcg ggtatggggt cgtgcccatt gagaattcga
     4801 ctgaaggggc gattggccga acgctggatc tgctgttgtc cacgcaactg cgcttgtgcg
     4861 gcgaagtgct gttgccggtc catcagaatc tgctttcgaa aggcacggac attgccgccg
     4921 ttacccgtat cttttcccat gcgcagagcc ttgcccagtg caaccgctgg ctcgcacagc
     4981 actgtccgcg cgcagaacgg gtaccggtta ccagcaatgc ggaggccgcg aaaatggcgc
     5041 aggaagccga tttttccgca gccatcgcgg gcagatccgc tgctgcacac tatggtctgg
     5101 cggtgctggc cgacaatatc gaagatgagc caaacaatac gacccgattc gtcgtgctat
     5161 ccgctcagga gccagcacca tcgggcaacg acaagacatc cctggtcatg tccacacgta
     5221 acgtacccgg agccatccac gaattgctga ccccgctggc aaataacgga gtcagcatga
     5281 cgcggttgga atcacgtccg tcccgcaccg gattgtggga gtacgttttc tttgttgaca
     5341 tggaaggcca tcaaaacgag gaacatgtgg cgcgtgccct caaggagatg agggaattgg
     5401 ccgccttcct caaaattctg ggctcgtatc ccatcgccgt ccaatgagcc ccgtcaccgc
     5461 agagcagctc gtggacgttt gcgaacgtgc accagcgtat gtccgcgcaa tcgcacctta
     5521 ccagcccggc aagccgatct ctgaactcgc tcgcgagatg ggtctgcagg agagttccat
     5581 cgtaaaactg gcgtcgaacg aaaatccccg aggagccagt ccgaaagcga ttgctgccat
     5641 gcgcatcgcg attggtgcta cgggacgtta tccagacggc aacgcttatg cacttaagga
     5701 tgcgcttacg aagcggtatg gcgtggcgca cgatcgcatc gtgctgggca acggttccaa
     5761 tgacgtgctc gagatggctg cgcgcgcttt cctctcgccc aaagcgtcgg ccgtgtattc
     5821 ggaacacgcg tttgttgttt attcattggc gacccaggcg gtgggtgcga ccggcattgt
     5881 ggtgcccgca cataattttg gtcacgacct cgatgcgatg tccaaggcga tccgaagcga
     5941 tacgcgcgtg gtgtttatcg ccaatccgaa caatccgacc ggaacctggt tgccaccgga
     6001 gcagatccac gggttcctga ccaaaacccc agcggacgta ctggtcgtgc tggacgaagc
     6061 ctataacgaa tatctggacc ccgacttacg cagcaatgcg gttgcctgga gcgaagaatt
     6121 tccaaacctc gtcgtatctc gcaccttttc gaaggcgtac gggcttgccg gattgcgggt
     6181 gggttatgga ttttcccatc caggtgtcat tgatttgatg aaccgggtac gtcagccgtt
     6241 taatgtaagc gatattgggc aggttggcgc cgccgccgca ttattcgacg atgaattcgt
     6301 ccagcaaagt acgttgctca accggcatgg gatgaaagca cttaccgaag cattcaccag
     6361 tctgggcttg tcctggattc cgtcgcatgg caatttcgta tgcgtcaagg tcggaaaagc
     6421 cgcagaagtt ttccagcggt tgctcaagca gggtgtcatt gtgcggccta tcgtctccta
     6481 cgggatgccg gagcacctgc gcgtttcaat cgggaccgag tccgaaaatg cccgctttct
     6541 tgcggcgctt gcagtatcca ttcagtgaag tcgatcgcat cgtcctgaat aagcgcgcat
     6601 cagtcttgaa gaaattcaat tgcgacaaac tggttgtaat cggcgtcgga cttattggcg
     6661 gttcggtagc aaccgcactg aggcgtgcca acagcgtgcg gcgcattgtt ggagtggg
//
LOCUS       DATMZQ010000424         7335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_685663, whole genome shotgun sequence.
ACCESSION   DATMZQ010000424 DATMZQ010000000
VERSION     DATMZQ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685663"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VN496_13480"
     CDS             complement(<1..854)
                     /locus_tag="VN496_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXF18014.1"
                     /translation="MSLASEADYDVVIIGSGVGGGAIALSLAKTGARVLILERGERLP
                     REAENWSPEAVFVEQRYRTRETWYDWDGQPFRPGQFYFVGGHTKVYGTAMFRFRERDF
                     EGIEHAEGTTVPWPIRYADLEPWYGEAEQLFGVHGEAGEDPTEPPRSTPYPHAPIPHE
                     PIIAKAFERMRALGLHPFHMPSAVDLHAGGSCIRCGTCDAFPCRIGAKGDAETRLIDP
                     ALKHANVALQTGSEVTRLVADDNGKRIVGAHYRRNGATASVSARLFVLSAGAINSAAL
                     LLRSADTR"
     gene            complement(851..1966)
                     /locus_tag="VN496_13485"
     CDS             complement(851..1966)
                     /locus_tag="VN496_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18015.1"
                     /translation="MAALDLINVEKSFGSNKILHDISVSLAHGEFLVLVGPSGCGKST
                     LMNIIAGLEEPSGGQLRLDGRDITPLAPADRNISMVFQSYALYPNMTVAKNIEFPLAM
                     RKVEKSKRAERVKSVARLLQIEHLLDRKPRQLSGGQRQRVAIGRALAREPQLYLFDEP
                     LSNLDAQLRLDMRTEIKKLHHRLGATIVYVTHDQIEAMTLATRIAVMKGGVLQQLGSP
                     YEVYNQPANMFVAGFMGSPRMNLIKSRLAQANGSLALEINNADHPLVRIPLPAPSAAL
                     SAYAGRDVFAGIRAEAVRLARDGMAPTATECMFDARVEVIEPTGADTLVVLDLGGKEF
                     TVRLEPDVQLKPGQDARFLVELSKLVCFDADTERRIA"
     gene            complement(1972..2841)
                     /locus_tag="VN496_13490"
     CDS             complement(1972..2841)
                     /locus_tag="VN496_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXF18016.1"
                     /translation="MRDHRASGIPLERILLYAVLFFLAVMFLLPVYVMVCNSLKPLDE
                     IRSGNLMSLPVQWTVDPWFTAWSTAQIGVQPTGLRPYFYNSFLLVIPAVVISTLIGAL
                     NGYILTQFRFRGANALFAAMLFSVFIPFQIVLIPMAKTLGILGMAGTVKGLIFVNSVY
                     GIGFTTLFFRNYYAAFPQELVRSARIDGADFFGVLFRILLPSSAPIIVVTVIWQFTNI
                     WNEFLFGASFSDYSSFPITVALNNLVNSSTGVKEYNVHFAGAFIAAIPTLIVYLLSGK
                     YFVRGLMAGSVKG"
     gene            complement(2834..3700)
                     /locus_tag="VN496_13495"
     CDS             complement(2834..3700)
                     /locus_tag="VN496_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXF18017.1"
                     /translation="MRAGALPKLLLSPSVLLVLVCVYGYIMFTLYLSFTTSMMMPSYE
                     WAGTTNYQRLFVLENWRVSLGNLWIFALLYIGVAIALGLGFAIMIDQHIRAEGAFRSI
                     FLYPMALSFIVTGTAWKWLLDPGVGLEHTLRDLGWQSFTFGWIKDSDMAIYCVVIAAV
                     WQTTGFVMALFLAGMRGVDAEQVSAARVDGARSWQIYLHIIIPQLGPVFVSAFVILAH
                     MAIKSYDLIIALTNGGPGRSTWLPSVFMYQYSFTRNEMAVGAASAVMMLAGIAVVVLP
                     YLYSEMRKVKNA"
     gene            complement(3770..5011)
                     /locus_tag="VN496_13500"
     CDS             complement(3770..5011)
                     /locus_tag="VN496_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18018.1"
                     /translation="MHRKIIATVIAAAFGCAGMASAADSVEVLHWWTSGGEAAALNVL
                     KTNLEKQGVTWNDMPVAGGGGEAAMTAVRARVTAGNPPTAVQMLGFDIQDWGKQGVVA
                     DLNSVAGKEGWDKTVPQALQRFSKYNGKWIAAPVNVHSTNWVWANKEVLAKAGVTEPK
                     TWDEFIAAAEKIQKAGFIAIAHGGQPWQDATIFDGVAISTGGVDYYRKAFIDLDQKAL
                     NSPTTEKVFQRMSQVRKLVDKDFSGRDWNVASGMVISGKAGFQMMGDWAKGEFINAKK
                     VPGKDFLCFRTPGTQGVVSFNSDQFVMFKVGADKTAAQNKLASAIMEPGFQSAFNVVK
                     GSVPARTDVPDTAFDACGKKGMKDLAEANKNNTLVGSMAHGHAVPASIKNAIYDVVTR
                     HFNGQLDDKKAVAELAAAVKK"
     gene            complement(5165..5953)
                     /locus_tag="VN496_13505"
     CDS             complement(5165..5953)
                     /locus_tag="VN496_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXF18019.1"
                     /translation="MPESLASQVKFHIDGAIATVTLDRAEKLHALTPGMIETLADIAT
                     QVDGDVAIRCVILTAAGDKAFCVGADINAWAGLDPLDMWRRWIRRGHQVFDQWARLRV
                     PVIAAINGHAFGGGLELAAIADIRIAAGSANFGLPEATIATCPGWSGTQRLAALIGAS
                     RVKYLALTGQRIDATEAWRLGIVHEHVAASQVMQRARELAAGICNLAPVSVQLTKQII
                     DAGQGMGLSGALEAMAGALAATTADAREGVASFREKRAPVYRGE"
     gene            complement(5946..>7335)
                     /locus_tag="VN496_13510"
     CDS             complement(5946..>7335)
                     /locus_tag="VN496_13510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXF18020.1"
                     /translation="IDHLARPGMLKTVLAGSYPSGPSSLPMPEIWRQIVEDEIAAYNL
                     PSGILFDMHRDAAAKRPGVLTKVGLDTFVDPARQGGAMNKRASTQPVVKKVTFAEEEW
                     LFFPAIAPRVAILRATSADERGNLSFEHEGAFLGAMDQALAARNNGGVIIAQVKRVVG
                     SGSLKPQMVHVPGTLVDYIVVDPEQMQTTQTFYDPAISGEITRPENSFELQDWGPEKV
                     IARRAAMELTSGSAVNLGFGISANVPRVLLEEGLHGEVTWVIEQGAVGGVPLLGFAFG
                     CAANAEAIVSSPDQFTYFQGGGFDVALLSFLQIDRDGSVNVSKLGAKPYLTAGCGGFV
                     DITAHAKRIVFSGFFTAGAKLEVGDGRLTILQEGRSKKLIEAVEHVTFSGRMARQRGQ
                     HITYVTERCVIDLTGDGLTVREIAPGIDLKRDVLDQAAFALKVDPQLKPMDAALFRNA
                     PLNRKLKAPAYA"
BASE COUNT         1472 a         2203 c         2175 g         1485 t
ORIGIN      
        1 ttgcgcgtgt cggcagagcg cagcagcagg gccgcgctgt tgatggcgcc ggcggacagc
       61 acgaacagcc gcgcgctgac cgacgccgtt gccccattcc tgcggtagtg cgcgccgacg
      121 atgcgtttac cattgtcgtc ggcaacaagc cgggtgactt cgctgccggt ctgcaatgca
      181 acgttcgcat gcttcaatgc cggatcgatc agcctggtct cggcgtcccc ctttgcacca
      241 atccgacagg ggaaggcatc gcatgtgccg cagcggatgc aactaccgcc ggcatgcaga
      301 tcgaccgcgg aaggcatgtg aaaggggtgc aggcccagtg cacgcatgcg ctcgaatgcc
      361 ttcgcgataa tgggttcgtg cggaatcggc gcatgcggat agggcgtgga gcgcggcggt
      421 tcagtcgggt cctcgcctgc ctcgccgtgc acgccaaaca attgctcggc ttcgccgtac
      481 cagggttcca gatcggcgta acggataggc cacggcacgg tagtgccttc ggcatgctcg
      541 ataccctcga aatcgcgctc gcgaaaacgg aacattgccg tgccgtagac cttggtatgg
      601 ccgccgacga agtaaaactg tccggggcgg aagggttggc cgtcccagtc ataccaggtt
      661 tcacgagtgc ggtagcgctg ctcgacgaat accgcctccg ggctccagtt ctcggcttcc
      721 ctgggcaggc gttcgccccg ttccagaatc aagactcttg cgccggtctt cgccagtgag
      781 agcgcgatcg ctccgccacc tacgccggag ccgatgatga cgacgtcgta gtccgcctct
      841 gacgccaggc tcaagcgatg cgcctttcgg tgtcggcatc gaagcacacc agtttgctga
      901 gttccaccag gaaacgcgca tcctgtcccg gcttcagctg cacatccggc tcgagtcgca
      961 cggtaaattc cttgccgccg agatcgagca ccacgagtgt gtccgcgccg gtcggttcga
     1021 taacctcgac gcgggcatcg aacatgcatt cggtcgcagt cggcgccatt ccgtcgcgcg
     1081 cgagtctgac ggcctccgca cggatgcccg caaaaacatc cctgcccgcg tatgcactca
     1141 atgcggcgga cggcgcaggc agcggaatgc gtaccagcgg gtggtctgca ttgttaattt
     1201 ccagcgccag gctgccgttt gcctgtgcga gccgcgattt gatcaaattc atgcgcgggg
     1261 aacccatgaa tcctgccacg aacatattcg cgggctggtt gtagacttcg tacgggctgc
     1321 cgagctgctg caacacgcct cccttcatga ccgcgatgcg tgtggcgagc gtcattgctt
     1381 cgatctggtc atgcgttacg tagacgatgg tggcgcccag tctgtggtgc aatttcttga
     1441 tttccgtgcg catgtcgagg cgcaactgcg cgtccaggtt ggacagcggc tcatcgaaca
     1501 gatagagctg gggctcacgc gccagcgcgc gtccaatggc aacgcgctgg cgctgtccgc
     1561 cggaaagctg gcgcggcttg cggtccagca gatgttcaat ctgcagcagc cgggccacgc
     1621 ttttcacgcg ttccgcgcgc ttgctcttct ccactttacg catggcgagc ggaaattcga
     1681 tgttcttggc caccgtcatg ttgggataga gcgcataaga ctggaacacc atcgaaatgt
     1741 tgcgatcggc tggagccagc ggggtgatgt cgcgtccatc cagccgaagt tgtccgccgc
     1801 tgggttcctc cagccccgcg ataatgttca tcagcgtgct tttgccgcag ccggacggtc
     1861 ccacgagcac caggaattcg ccgtgggcga ggcttaccga aatgtcgtga agaatcttgt
     1921 tggagccgaa gctcttctcc acattgatga gatcgagtgc tgccatttaa gtcatccttt
     1981 gaccgaacct gccatcaagc cgcgaacgaa atatttgccg gacaggagat agacgatcaa
     2041 ggtcgggatg gcggcgatga aggcgccggc aaaatgaacg ttgtactcct tgacgccggt
     2101 ggagctgttg accaggttgt tgagcgcaac cgtgatcggg aaagaactgt aatcgctgaa
     2161 agaagcgccg aacagaaatt cgttccagat gttggtgaac tgccagatga cggtcacgac
     2221 gataattgga gcgctgctgg gcagcagaat gcgaaacagc acgccgaaga aatccgcgcc
     2281 gtcgatcctt gccgagcgca ccagctcctg cggaaaagcc gcgtaatagt tgcggaagaa
     2341 cagcgtggtg aaacctatcc catagaccga gttgacgaaa atcagcccct ttaccgtgcc
     2401 ggccatgccg aggataccaa gcgtcttcgc catcggaatc agcacgatct ggaacgggat
     2461 gaataccgag aacagcatgg cggcgaacag cgcattcgcg ccacgaaagc gaaactgggt
     2521 gaggatgtag ccgttcagtg caccaatcaa cgtggaaatg actacggctg gaatgaccag
     2581 aagaaatgaa ttgtagaaat acggcctgag cccggttggt tgcacgccga tctgggcggt
     2641 gctccaggcg gtgaaccagg gatccaccgt ccactgcacc ggcagggaca tcagattgcc
     2701 gctgcgtatt tcatcgagcg gctttagcga attgcacacc atgacgtaca cgggcagcag
     2761 aaacatcacg gcgagaaaaa acagaaccgc ataaagcagg atgcgctcca ggggaatacc
     2821 ggacgcgcgg tggtcacgca ttttttacct tgcgcatttc actgtagaga tacggtagta
     2881 ccaccactgc gatgccagcc agcatcatta cggcgctggc ggctccgact gccatttcgt
     2941 tgcgggtgaa actgtactgg tacatgaata ctgatggcag ccaggtggag cggcccggtc
     3001 cgccattggt cagcgcaatg atcaggtcat aagacttgat cgccatgtgg gcgagaatca
     3061 cgaacgccga tacgaacacc ggtccgagct gcggaatgat gatgtgcaga tagatttgcc
     3121 aggagcgcgc accgtcgact cgggcggcgc tgacctgctc tgcgtcaact ccgcgcatcc
     3181 cggcgagaaa cagtgccatg acaaaaccgg tggtttgcca cacagcggcg atgacgacgc
     3241 aatagatcgc catgtcgctg tccttgatcc agccgaaggt gaaactctgc cagcccaggt
     3301 cgcgcagagt gtgctcgagt ccgacgcccg gatcgagcag ccatttccac gccgtgccgg
     3361 tgacgatgaa cgacagcgcc atcggataaa ggaaaatcga acggaacgcg ccttcggccc
     3421 tgatgtgctg gtcgatcatg atcgcgaaac caagtccgag cgcgatggca acgccgatgt
     3481 agagcagcgc aaagatccac agattgccga gtgagactcg ccagttctcc aaaacaaaaa
     3541 gccgttggta attcgtcgtg ccagcccatt cgtaggacgg catcatcatc gatgtcgtga
     3601 acgacaggta aagcgtgaac atgatgtacc cgtatacgca taccagcacg agcagcacac
     3661 tcgggctgag cagaagtttg ggcagggcgc ccgcgcgcat gggtcagatt ctcgcaggtc
     3721 cgggcggcac ggcaggtgct gcccggagcc tttgcaaaat gccgctacat tacttcttga
     3781 ctgcagctgc cagttcggca accgccttct tgtcgtcgag ttggccgttg aaatggcggg
     3841 tcacgacgtc atagatggcg ttcttgatcg aggcggggac ggcgtgtcca tgcgccatcg
     3901 agccaaccag cgtgttgttt ttgttcgctt cggccagatc tttcatgcct ttcttgccac
     3961 aggcatcgaa tgccgtgtcg ggtacgtcag tgcgcgccgg gacggaccct ttcacaacat
     4021 tgaacgctga ctgaaagcca ggttccatga tggccgacgc cagtttgttt tgggcagcgg
     4081 ttttgtctgc tccgactttg aacatcacga actgatcgga attgaacgaa accacgcctt
     4141 gcgtaccggg cgtgcggaag cagaggaagt ccttgcccgg cactttttta gcattgatga
     4201 attcaccctt ggcccaatcc cccatcatct ggaagccggc cttgccgctg atgaccatgc
     4261 cggaggcaac gttccagtcc cggccggaga aatccttgtc gaccagcttg cgcacttgcg
     4321 acatgcgctg gaacactttt tccgtcgtcg gcgagttgag cgctttctgg tcaagatcga
     4381 tgaacgcctt gcggtaatag tccacgccgc cagtggaaat cgccacgcca tcgaatatgg
     4441 ttgcgtcctg ccatggttgg ccgccatgtg caattgcaat gaatccggct ttctgtatct
     4501 tttccgcggc cgcaataaat tcgtcccagg tcttgggttc cgtgacgccc gctttggcga
     4561 gaacttcttt gttggcccaa acccagttgg tcgagtgcac attcaccggc gcggcaatcc
     4621 acttgccgtt gtatttggag aatctctgca atgcctgcgg cactgtttta tcccaccctt
     4681 ctttgccggc gacggaattc aagtccgcga ccacaccctg tttgccccag tcctgaatgt
     4741 cgaacccaag catctgcact gcagtcgggg gattgccggc cgtgacacgg gcgcgtactg
     4801 cggtcatggc tgcttcgccg ccgccgcctg ccaccggcat gtcgttccag gtgacaccct
     4861 gcttttcgag attggtcttc agcacattca gagcggccgc ctctcctccg gacgtccacc
     4921 agtgcagtac ttcgactgaa tcagccgcgg atgccatacc ggcgcaacca aatgcagcgg
     4981 caatcaccgt ggcaattatt ttcctatgca tgttctcctc ctgtttaagt cttgtatgtc
     5041 cgattgcggc cggacaattg ccgtttattg agcgaccaca gccctcagtg tgccaaatta
     5101 ccccgttggc caccatcgct gctgtatgca tctggagtaa acagtgcggc ggtcttgctt
     5161 catgtcactc gccccgatag accggcgcgc gtttttcacg aaaactcgcc acgccttcgc
     5221 gggcgtccgc tgtcgtagcg gcaagggcgc ctgccatggc ttccagtgcg ccggacaatc
     5281 ccattccctg gccggcgtcg atgatctgct ttgtcaactg cactgacacg ggcgcaaggt
     5341 tgcaaatgcc ggccgcaagt tcgcgagccc gctgcatcac ctgcgaggcc gctacatgtt
     5401 catgcacgat ccccagacgc caggcctcgg ttgcgtcgat gcgctgccca gtcagagcaa
     5461 gatacttcac ccggctggcg ccaatcagcg cggccagccg ttgtgtgcct gaccagcccg
     5521 ggcaagtagc aatggtcgcc tccggcagtc cgaagtttgc cgacccggcc gcgatgcgga
     5581 tgtccgcgat ggcggcaagc tcgagtccgc cgccaaatgc atgcccattg attgctgcaa
     5641 tgaccggcac gcgcaggcgc gcccactggt caaaaacctg gtgcccgcgc ctgatccagc
     5701 ggcgccacat gtcaagggga tccaggcccg cccaggcatt aatgtctgcg ccgacgcaga
     5761 acgccttgtc gccagcagcg gtcagaatga cgcaacggat ggcgacgtcg ccgtcaactt
     5821 gcgtggcgat atcggccagc gtttcgatca ttcccggcgt gagcgcatgc agcttctccg
     5881 cgcgatcgag cgtgacggtc gcaatcgctc catcgatgtg gaatttcacc tggctggcca
     5941 aagattcagg cataagcagg cgctttcagt ttgcggttca gcggcgcatt gcggaatagc
     6001 gcggcgtcca ttggtttgag ctgcgggtcg actttcagcg caaaagctgc ctggtcgagc
     6061 acgtcccgtt tcaaatctat gcccggtgcg atctctcgca ccgtcaaacc atcccccgtc
     6121 agatcgatga cgcaacgttc ggtgacgtag gtgatgtgct gtccccgctg gcgcgccatc
     6181 ctgccgctga aagtcacgtg ctcgacagct tcgatcagct tcttgctgcg accttcctgc
     6241 aagattgtca gtcggccgtc gccgacttcg agtttcgcgc ccgcggtgaa gaaaccggaa
     6301 aatacaatcc gcttggcatg ggcggtgatg tcgacgaatc cgccgcagcc cgcagtcagg
     6361 taaggtttgg cgccgagctt ggaaacattg acgctgccgt cgcgatcgat ctgcagaaag
     6421 gacaacagcg cgacatcgaa tccgccaccc tggaaataag tgaactgatc gggtgaagag
     6481 acaatggcct cggcattggc cgcgcagccg aaggcgaatc cgagcaatgg cacgccgccg
     6541 actgcgccct gctcgatgac ccaggtgact tcgccgtgca ggccctcctc gagcagaacg
     6601 cgcggcacgt tggcggaaat cccgaagccg aggttcacag cggaaccgga ggtgagctcc
     6661 atcgcggcgc gcctggcgat gactttttcg ggaccccagt cttgcagttc aaaactgttt
     6721 tcgggccggg taatctcgcc gctgatggct gggtcgtaaa aggtctgcgt ggtctgcatc
     6781 tgctcagggt cgaccacgat gtagtccacc aacgtgccgg gcacgtgcac catttgtggc
     6841 ttgagcgagc cagaacccac tacgcgtttg acttgcgcga tgatcacgcc gccgttgttg
     6901 cgggcagcaa gcgcctggtc cattgcaccg aggaacgcac cctcgtgctc gaaggacagg
     6961 ttaccgcgct catccgcgct ggtggcacgc agtatcgcaa cgcgcggtgc gatcgccgga
     7021 aaaaacaacc attcctcttc ggcgaacgtt acttttttca ccaccggttg cgtcgatgca
     7081 cgtttgttca tcgcgccgcc ctggcgcgcg ggatcgacga aggtgtcgag tcctactttg
     7141 gtgagcacgc cgggacgttt tgctgctgca tcccggtgca tgtcgaacag gatgccgctc
     7201 gggaggttgt atgcggcgat ttcatcttcg acgatctggc gccagatttc cggcatcggc
     7261 agggaggaag gcccgctcgg gtaggagcct gccagcactg tctttagcat tccgggacgg
     7321 gccagatgat cgatg
//
LOCUS       DATMZQ010000425         5587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4139159, whole genome shotgun sequence.
ACCESSION   DATMZQ010000425 DATMZQ010000000
VERSION     DATMZQ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4139159"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..939)
                     /locus_tag="VN496_13515"
     CDS             complement(19..939)
                     /locus_tag="VN496_13515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HXF18021.1"
                     /translation="MTTAQRTPRFIAAGEALTDLIREEEKIWVARAGGAPWNVARVLA
                     RLGIPSAFAGSVSRDAFGEELAALSRDAGLDIRFLQQYDKPPLLAIVQELSPPNYFFL
                     GVDSADLAFNPAALPQDWLRSVEWMHCGGISLTREPLSSRLIDLLHAAKGAGAHISYD
                     PNFRNLMTARYDATLARVAGLADVIKVSDEDIAGLFRTDDRQLAMAKLKSLNPSAAIL
                     LTLGAQGAELHAQGQILRQASVQVKVADTVGAGDASMGGLLFSLMTHRASDWRTHLRW
                     AVAAGCAACMQSGAVPPPLPTIESLLATMQ"
     gene            complement(936..2105)
                     /locus_tag="VN496_13520"
     CDS             complement(936..2105)
                     /locus_tag="VN496_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXF18022.1"
                     /translation="MQLTLPDDHGALHAYRLSHASSPFATVAPTRWNRSAYAAAHVVV
                     DPLQPCDPWDGPPIIDWEATLRFREHLWRLGFKVAEAMDTAQRGMGLDWPGARELIRR
                     SVAHAKTIPGADLACGVGTDQLVANERTTIADVMKAYETQIEAVEAAGGRIILMASRA
                     LAAVAKSADDYSRIYGELLRQCKEKVVLHWLGEMFDPALKGYWGTNDIAAAMDTVLGI
                     VRENAQKIEGIKISLLDARWEVAMRSNLPAGVIMFTGDDFNYDTLIAGDGKHHSHALL
                     GIFDPIAHVAAAALSKLASGDVEGYKKSIVPTIPLSREIFRAPTRHYKAGVIFIAWLN
                     GHQSHFVAAGGLQAARSVRHYTKVFELADRCGALIDPDLAVARMRSFLQVHAGIS"
     gene            complement(2115..3725)
                     /locus_tag="VN496_13525"
     CDS             complement(2115..3725)
                     /locus_tag="VN496_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXF18023.1"
                     /translation="MTETGYDYIITGGGSAGCVLANRLSADPSVKVLLLEAGGSDRHP
                     FFHLPAGFAKMTWGIGAWGWSTVPQAHLKDRVLRFTQAKVIGGGSSINAQIYTRGAPA
                     DYDAWDRDAGARGWSYRDVLPYFKRAENNERYANEYHGYGGPLGVSNPISPLPICEAF
                     FQAGQELGIPFNPDFNGAHQEGLGYYQLNQSNARRSSTSVAYLKPIRNRRNLTVRLRT
                     HALRVVVEHGRATGVEIANSGSASPELIRAVREVIVTSGAIGSPKLLMQSGIGPAAHL
                     GSVGVKVVHDLPGVGGNLQDHLDLFVIGECTGDHTYDKYNRPHHAALGGLQYLLWKKG
                     PVASSLYETGGFWYAEKGARSPDIQFHLGLGSGIEAGVAKMRNPGVTLNSAYLRPRSR
                     GTVRLASVDPNAAPLIDPNYWAEPRDRELAIEGLRLAREILHQPALKRFVLGERLPGD
                     ALTSDAELFDYACAHAKTDHHPVGTCRIGPANDSASVVGFDLRVMGLDGLRVADASVM
                     PFIPSSNTNAPTIMVAEKAADHILGRLA"
     gene            complement(3803..4573)
                     /locus_tag="VN496_13530"
     CDS             complement(3803..4573)
                     /locus_tag="VN496_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HXF18024.1"
                     /translation="MAQSRPVALVTGARRGIGAAIALALARTGFDVTITDIVQDDDVQ
                     ATLAALRASGAKAVFIRSDLADVADHGRVVDEAVAKCGPIDCLVNNAGVPSPARGDLL
                     EISPLAFDQVLQINLRGTFFFTQQVARHMAMTAARNPRSIVTISSVSAELASPERGEY
                     CMSKAGLAMLTKLFALRLARHGIAVFEVRPGVIRTPMTATISEKYDQRIADGLVPMGR
                     WGEPGDIGAAVAGLAGGNFQFATGSVIDVDGGLAIPRL"
     gene            complement(4575..>5587)
                     /locus_tag="VN496_13535"
     CDS             complement(4575..>5587)
                     /locus_tag="VN496_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXF18025.1"
                     /translation="LGLHPFHMPSAVDLHAGGSCIRCGTCDAFPCRIGAKGDAETRLI
                     DPALKHANVALQTGSEVTRLVADDSGKRIVAAHYRKNGATESVSARLFVLSAGAINSA
                     ALLLRSADTRNPNGLANSSGVVGRYYMNHNCTGIMAIKPFQVNQTRFPKTLAVNDFYF
                     ASKADDKPLGNLQLLGKIQEPMLRSAMPHAPKRLRGWLARHSVDWYVMSEDLAQHDSE
                     VRLRNDGAIELRWKRSNLRAHRHFVKVAKKLLHDIGFPIVLSQPFGTDTPSHQCGTAR
                     FGTDPKASALDPFCRAWDHDNLYVVDASFFPSSAALNPALTIAAQALRVGSHLKARLH
                     EF"
BASE COUNT         1100 a         1720 c         1699 g         1068 t
ORIGIN      
        1 cggaaatccg gcatgcggtt attgcattgt cgccagtagg ctttcaattg tgggcaacgg
       61 gggcggcacg gcgccggact gcatgcatgc cgcgcatcct gctgcgaccg cccagcgaag
      121 gtgggttcgc cagtctgaag cgcgatgggt cataaggctg aacagcaatc cgcccatgct
      181 ggcgtcgcca gcgcccaccg tatcggctac tttcacctgc acagaagcct gccgcaaaat
      241 ctgtccctgc gcatgcagtt cagcgccttg cgcgccgagc gttagcagga tagccgcact
      301 cggattgagc gatttgagtt tcgccatcgc aagttgccgg tcgtcagttc ggaacagacc
      361 tgctatatcc tcatcggata ccttgattac atcagccagg ccggcaacac gggccagcgt
      421 tgcatcatag cgggccgtca tcagattgcg aaagttcggg tcgtagctga tgtgcgcgcc
      481 ggcgcccttt gccgcgtgca gcaaatcgat caaccggctg ctcagcggtt cgcgagtcaa
      541 gctgataccg ccacaatgca tccattcaac cgaacgcagc cagtcctgcg gaagcgctgc
      601 cggattgaac gcgagatcag cgctgtcgac gcccagaaaa aaatagttcg gaggagacag
      661 ttcctgaacg atggccagca gcggcggttt gtcgtattgc tgcaggaatc ggatatccaa
      721 accggcatcc cggctcaagg ccgccagttc ttcaccaaaa gcgtcacgac tcacgctacc
      781 cgcaaaggcg ctcggtatgc cgagtcgagc caggacacgc gcgacattcc agggtgcgcc
      841 tcccgctcgt gccacccaga ttttttcttc ctcccggatt aaatcggtca acgcttcccc
      901 ggcggctatg aagcgcggcg tgcgctgcgc tgtggtcatg agatgccggc gtgcacctga
      961 aggaaactgc gcatgcgcgc gaccgcaagg tcgggatcga tcagtgcgcc acagcggtcc
     1021 gcgagttcga acacctttgt gtaatgccgc acggagcgag ccgcttgcag tccacccgcg
     1081 gcgacgaaat gcgactggtg gccgttcagc cacgcgatga agataacccc agccttgtaa
     1141 tggcgggtag gcgcacggaa tatttcgcgc gagagcggaa tggtcgggac aatgctcttt
     1201 ttgtaacctt cgacgtcgcc gctggcgagc ttgctcagtg cggctgcggc cacgtgtgcg
     1261 atcggatcaa aaatccccag cagcgcatgt gaatgatgtt tgccgtctcc ggcgatcagc
     1321 gtgtcgtagt tgaaatcgtc gccagtgaac atgatcacgc cggctggcag atttgagcgc
     1381 atggcgactt cccaccgggc gtcgagcagg gaaatcttga tgccttctat cttctgcgca
     1441 ttctcgcgca cgatgccaag caccgtgtcc atggctgcgg caatatcatt tgtgccccag
     1501 tagcccttca gtgccgggtc gaacatctct cccagccaat gaagcactac tttttccttg
     1561 cattggcgca gcagttctcc gtaaatacgc gagtaatcgt ccgcgctttt cgccacagcc
     1621 gccaatgcac ggctcgccat gaggatgatg cgtccaccag ccgcttccac tgcttcgatt
     1681 tgtgtctcat aagctttcat cacgtctgcg atcgtggtgc gctcgttggc aaccagctga
     1741 tctgtaccca cgccgcaggc aaggtccgcg ccgggaatgg ttttagcatg cgcgacgctg
     1801 cggcggatga gttcgcgcgc tccgggccaa tccagaccca tgccgcgctg cgccgtgtcc
     1861 atggcctcgg cgactttgaa gcccaggcgc cacaaatgtt cacggaagcg cagcgtcgct
     1921 tcccagtcga ttatgggcgg accgtcccag ggatcgcagg gctggagcgg atcgaccacg
     1981 acatgcgcag cggcgtaagc gcttcggttc catcgcgttg gcgcgactgt ggcgaacggc
     2041 gaggatgcat ggctcagacg gtaggcatgc aaggcgccat gatcatcggg caaagtcagt
     2101 tgcatggcga ttccttaagc gaggcgtccg agaatatggt ccgcggcctt ctcggcaacc
     2161 atgatggtgg gcgcattggt attggaagaa gggatgaaag gcattaccga ggcgtccgcg
     2221 acgcgcagcc catcgagacc cattacacgc agatcgaaac cgaccacgct ggcagaatcg
     2281 ttggcgggtc cgatgcggca agtccctacc ggatggtgat cggtcttggc gtgcgcgcag
     2341 gcgtagtcga aaagttcggc atcgctggtt agtgcatcgc caggcaggcg ttcgcccagg
     2401 acgaaccgtt tcagcgcagg ttggtgcagg atctcgcgtg ccagacgcaa cccttcgatc
     2461 gccagttcgc ggtcgcgcgg ttcagcccaa tagttgggat cgattagcgg tgcggcattc
     2521 ggatcgacgc tggccaaacg cacggtgccg cgcgagcgcg ggcgcaagta agcgctattg
     2581 agcgtaacgc caggattgcg catcttcgcg accccggcct cgatgccgga accgagaccc
     2641 agatgaaatt ggatatcggg tgagcgcgcg cctttctccg cataccagaa gccgccggtt
     2701 tcgtacaggc tggaggccac cggtcccttt ttccacagca gatactgaag accgcccaac
     2761 gcggcgtgat gcggccggtt gtacttgtcg taggtgtgat cgcccgtgca ctcgccaatg
     2821 acaaaaagat cgagatggtc ctgcagattg ccgcccaccc cgggcaggtc atggaccact
     2881 ttcacgccca ccgagccaag atgggcagcc ggtccgatgc ccgactgcat cagcagtttc
     2941 ggcgagccta tcgcgcccga agtcacaatg acttcgcgaa cggcacggat cagttccggc
     3001 gacgcagagc cagagtttgc aatctcaacg ccagtcgcgc ggccatgctc cacgaccacg
     3061 cgcaaagcat gcgtccgcaa gcgcacagtc agattgcgcc ggttgcggat cggcttcagg
     3121 tacgccaccg aagtcgatga gcggcgcgca ttcgactggt tgagctggta ataaccgagg
     3181 ccttcctgat gcgcgccgtt gaaatccggg ttgaacggaa tgccgagttc ctgtcctgcc
     3241 tgaaagaacg cctcgcagat cggcagcgga ctgatcggat tcgatacgcc cagtggcccg
     3301 ccgtaaccgt ggtactcatt ggcataacgt tcgttgtttt cggcccgctt gaaataaggc
     3361 agcacatccc gatatgacca gccgcgtgca ccggcatcgc gatcccaggc gtcgtagtcg
     3421 gcgggcgcgc cgcgcgtata gatctgcgcg ttgatcgacg aaccgccgcc gatgaccttc
     3481 gcctgtgtga accgcagcac gcgatccttc agatgcgcct ggggcaccgt cgaccagccc
     3541 caggcaccga tgccccaggt catcttggca aatcccgctg gcaggtgaaa gaaagggtgg
     3601 cggtccgaac cacccgcctc cagcagcagt actttcaccg acggatcagc actgagacgg
     3661 tttgcgagca cgcatcccgc tgagccgccg ccggtaatga tgtagtcgta tccggtctca
     3721 gtcatgggtg ccatcttcac catctgtgcc gggatagaac caatccagct tcatgagccg
     3781 cttacgcaga ggaggcacag tgtcaaagtc tcggaatggc caggccacca tcgacatcga
     3841 tgaccgagcc ggtcgcgaat tggaaatttc cgccggccag gcctgctact gcggcgccga
     3901 tgtccccggg ctcaccccag cgacccatgg gaacgagccc gtcggcaatg cgctggtcgt
     3961 atttttccga aatcgtcgcc gtcattggcg tgcgaatcac accgggacgc acttcgaaca
     4021 ctgcgatgcc gtgacgcgcc agtctcagcg cgaacagttt cgtcagcatg gccagccctg
     4081 cctttgacat gcagtattcg ccgcgctcgg gtgacgcgag ttctgcgcta accgaggaaa
     4141 tcgtgacaat cgaacgcgga ttgcgcgcgg ccgtcatcgc catgtgccga gcgacctgct
     4201 gggtaaaaaa gaacgttcca cgcaaattaa tttgcagcac ctggtcgaat gcaagcggac
     4261 tgatttccag cagatcgccg cgcgccggcg acggcacgcc ggcattgttg accagacaat
     4321 cgatcgggcc gcacttcgcg accgcctcat ccaccacccg gccatgatcc gccacgtccg
     4381 ccagatcgct gcgtatgaaa accgcttttg cgccggacgc acgcaacgcg gctagcgttg
     4441 cctgcacatc atcgtcctgc acgatatcgg tgatcgtcac atcgaaaccg gtacgcgcca
     4501 gtgccagtgc aatggcggcg ccgatgccgc gacgggcgcc ggtgaccaaa gcaactggac
     4561 gggattgggc catgtcagaa ctcgtgcagg cgcgccttca ggtggctgcc gacgcgcagc
     4621 gcctgggctg caatcgtcag cgccggattt agcgcggcag aggagggaaa gaagctcgcg
     4681 tcgaccacat acagattatc gtggtcccag gcgcggcaaa acggatccag tgctgaagcc
     4741 ttcgggtccg tgccgaagcg ggcggtgccg cactggtgcg acggtgtgtc agtgccgaat
     4801 ggctgcgaca gcacgatcgg aaagcctatg tcgtgcagca gcttctttgc gactttgacg
     4861 aagtggcggt gagcccgcag attgctgcgc ttccagcgca gctcgatagc gccatcgttg
     4921 cgcaacctga cttcgctatc atgctgcgcc aggtcttcgg acatcacgta ccaatcgacg
     4981 ctgtggcgcg ccagccagcc gcgcagccgt ttcggtgcgt gcggcatcgc actgcgcagc
     5041 atgggttcct ggattttccc cagcaattgc agattgccga gcggtttatc gtctgctttg
     5101 cttgcgaagt agaaatcgtt caccgcgagc gtttttggaa agcgcgtttg gttcacctgg
     5161 aagggtttga tggccatgat cccggtgcag ttgtggttca tgtagtagcg tccaaccact
     5221 cccgaggaat tcgccaggcc gttcggattg cgcgtgtcgg cagaacgcag cagcagggct
     5281 gcgctgttga tggcgcccgc ggacagcacg aacagccgcg cgctgaccga ctccgtggcg
     5341 ccgttcttgc ggtagtgcgc ggcgacaatg cgcttgccgc tgtcgtcggc gacgagcctg
     5401 gtgacttcgc tcccggtctg caaagcaacg tttgcatgct tcaatgccgg atcgataagc
     5461 ctggtctcgg cgtccccctt tgcaccaatc cgacagggaa aggcatcgca tgtgccgcag
     5521 cggatgcaac tgccgccggc atgcagatcg accgcggaag gcatgtggaa gggatgcagg
     5581 cccagtg
//
LOCUS       DATMZQ010000426        16756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4661239, whole genome shotgun sequence.
ACCESSION   DATMZQ010000426 DATMZQ010000000
VERSION     DATMZQ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16756
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4661239"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1513
                     /gene="murD"
                     /locus_tag="VN496_13540"
     CDS             131..1513
                     /gene="murD"
                     /locus_tag="VN496_13540"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXF18026.1"
                     /translation="MPIELTNRRALVLGLGASGLSMARWLSKRGAVVRVADSRDSPPF
                     ASTLRGELPAVELQTGAFRFESLDDIDLMAVSPGVPLAEPVIQRAVERGVEVVGDIEL
                     FARIRSEFPGSRVIAITGSNGKSTVTEMVGAMVRAAKIRTLVAGNIGLPILDAVLEVA
                     AGSRRPPDVFVLELSSFQLESTRTLNPDVAAVLNLSEDHLDRYSGMLDYATAKSRIFI
                     GAGIQVINRQDAWAKNMIGGGRPVLTFGLDEPWVDGNWGLRQMNDDLWLAEGRANLMK
                     VSELRVEGLHNAGNALAALALCRAIGLPYEPILDALFTFEGLPHRVHKVAEIKGISFY
                     DDSKGTNVGATVAALMGIAAKVVLIAGGEGKGQDFSPLRKAVTEKARSVVLIGRDRDK
                     IAAALEGSGVPIINAENMRDAVRVGFSLAQIGDAVMLSPACASFDMFSNYEHRAKVFI
                     DEVQALETGP"
     gene            1510..2682
                     /gene="ftsW"
                     /locus_tag="VN496_13545"
     CDS             1510..2682
                     /gene="ftsW"
                     /locus_tag="VN496_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXF18027.1"
                     /translation="MNGSLSLGVAAKSTSIAEFDYILISIAGLLLAMGLIMVYSSSVA
                     IAEGGRYTGNQATYYLVRHGIFLFVGIVAGFICFQFPLNFWEQAAPYLFCVGVALLMV
                     VLIPGLGREVNGSQRWLSLGLFNLQPSELMKLFAVLYAADYTVRKAALMHSFRKGFLP
                     MLVVMLLTGGLLLQEPDFGAFAVISAIAMGILFLGGMNWKLFATLILLLLLGFVVLIW
                     TSPYRLQRVTGFMDPWSDPFGKGYQLSHALIAFGRGEWFGVGLGASVEKLFYLPEAHT
                     DFLLAVIAEELGFCGVLTVMVLFGVMIWKAFAIGNQAARMERCFPGLVAQGIALWLGV
                     QTFINMGVNMGVLPTKGLTLPLLSFGGSGIVANCCALAVLLRVDYENRKMMMGYSL"
     gene            2822..3880
                     /gene="murG"
                     /locus_tag="VN496_13550"
     CDS             2822..3880
                     /gene="murG"
                     /locus_tag="VN496_13550"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXF18028.1"
                     /translation="MRTILIMAGGTGGHIFPALAVADQMRDSDWRVVWLGSKTGMESR
                     LVPQYGFEMEWVRFSGLRGKGILRALLLPINLLIAFAQSARAIFRVRPDVVLGMGGYI
                     SFPGGLMAALLARPLILHEQNSVAGLANKVLARFADRVLVAFPNALRNAEWTGNPVRR
                     DITQLVTPERRYRDRTGNLNVLVVGGSLGAQVLNETVPRALALMPPSGRPNVTHQAGE
                     QHLAMLEREYRLAGVSATALAFIEDMSSAYASADLVICRAGALTISEIAVAGVASVLV
                     PFAFAVDDHQTFNARYLSDRSAALLISQADFTARKLADVLLRCTRAKLLEMAIKAREL
                     GKPDAAQIVAQRCMAIAA"
     gene            3877..5274
                     /gene="murC"
                     /locus_tag="VN496_13555"
     CDS             3877..5274
                     /gene="murC"
                     /locus_tag="VN496_13555"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXF18029.1"
                     /translation="MKHKVKHVHFIGIGGAGMSGIAEVMLNLGFEVSGSDISENAATL
                     RLKNQGAKISIGHDASNVSGAHAVVTSAAVRQDNPEVAAARAKRIPVVPRAMMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAQAGMDPTFVIGGRLEAAGSHAKLGTGEFIVVE
                     ADESDASFLYLQPVLAVVTNIDADHMETYGHDFGRLRHAFLEFIEHLPFYGMAVLCAD
                     DANVKSLLPIITKQVTTYGIDEGAQVRAVEIHADAARMCFRVVRRNGNLKPAADLNIV
                     LNLPGLHNVRNALAAIAVGMEIGASDEAIVKALAEFRGVGRRFQRYGEVPLPQGSFTL
                     IDDYGHHPAEMAATISAARGAFPNRRLVLAFQPHRYTRTRDCFEDFVKVLSSVDALLL
                     TEVYPAGEAPIVAADGRALARAVRVAGKIEPVFVEQIVDLPAAILTKTTSGDVVITMG
                     AGSIGTVPGKLAKGN"
     gene            5290..6213
                     /gene="murB"
                     /locus_tag="VN496_13560"
     CDS             5290..6213
                     /gene="murB"
                     /locus_tag="VN496_13560"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXF18030.1"
                     /translation="MTDLIQIETAGLRGVYRRNEPMHTHVTWRAGGMADRAYTPADLD
                     DLGLFLRRLPQQEPILFVGLGSNLLIRDGGFRGTVVFTHRALAHLALESDSNPPEVYA
                     EAGVATPKVARFAANNNLEHAEFLAGIPGTVGGVLAMNAGCYGSETWDVVTRVRTMDR
                     AGKLHVRARREYEVAYRSVSPLSAPDEFFVAAWFGFARGDGEKARQRIRELLAKRIAT
                     QPLGEPNAGSVFRNPPHDYAARLIEASGLKGWRIGAAEVSSVHANFIVNKGGAIARDM
                     EQLISLVQNTVKHETGVTLEPEVRIVGEAWK"
     gene            6218..7132
                     /locus_tag="VN496_13565"
     CDS             6218..7132
                     /locus_tag="VN496_13565"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXF18031.1"
                     /translation="MNIKIGKVAVLMGGRSSEREISLLSGQAVLGALRQRGVDAHAFD
                     PKERELGDLKREKFERVFIALHGRYGEDGTVQGALELLGIPYSGSGVMASALAMDKWR
                     TKRVWLAEGIPVPSDLLVNESSDWDAVAKTLGLPLIIKPVHEGSTIGLTKVLTIKELP
                     AAYALAVKHDRMVLAEEFVDGRELTASILRETALPLIRIEAPQGNYDYQNKYFSDKTK
                     YHCPAGIASELEKTILEVALRAFNVLGCEGWGRADLILRADNTFSFLEMNTSPGMTSH
                     SLVPMAARQAGIAFPDLVLRILEAAHVG"
     gene            7122..7841
                     /locus_tag="VN496_13570"
     CDS             7122..7841
                     /locus_tag="VN496_13570"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HXF18032.1"
                     /translation="MWDDVATLRLSSNVLFALAAALVLYGAVDYVVHLKIYPLRDVRV
                     TGDISHVTREQVEAVLSRELRGNFFTVDLAHARQAFKKLPWVRAVNVRRQWPDRLDFE
                     VEEYRPIARWGSTALVSAEGEVFEAAVNSRLPVLQGPDGTAAEIVTRLQTFGKVLEPS
                     GRRVARITLSARRAWVLEFDDGMVLELGRDNLEARLSGFISAYGQTVARLPERAAYVD
                     LRYANGFAVRAVGLKSNKKKS"
     gene            7849..9093
                     /gene="ftsA"
                     /locus_tag="VN496_13575"
     CDS             7849..9093
                     /gene="ftsA"
                     /locus_tag="VN496_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXF18033.1"
                     /translation="MALSGRKENKNLIVGLDIGTSKIAAIVAEMKTEGGFEVVGMGSS
                     PSRGLKKGVVVNIESTVNAIQRALEEAELMADCKIRDVWTGIAGSHIRSFNSHGMVAI
                     KDREVAQSDVDRVVETAKAIPIPTDQQILHVLNQEFVIDGQEDVREPLGMSGVRLEVK
                     VHIVTGAVSAAQNIMKCVRRCGLEVNDLILQPLASSMAVLSEDEKDLGVCLVDIGGGT
                     SDIAVFTQGAIRHTAVIPIAGDQITNDIAMALRTPTKEAEEIKRRYGCALRELADPQE
                     MVEVPGVGERGSKKMSRQTLAEVVEPRVEELYSLVQAELRRSGFEELLSSGIVITGGS
                     AGMQGMVELGEEVFHMPVRLGLPSYAGGLAEVIRNPRYSTGVGLLMAGISQHQRHQIE
                     KLQSGSIKQIVGRMKKWFETNF"
     gene            9151..10305
                     /gene="ftsZ"
                     /locus_tag="VN496_13580"
     CDS             9151..10305
                     /gene="ftsZ"
                     /locus_tag="VN496_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXF18034.1"
                     /translation="MMFEIVDTQPQEAVIKVVGVGGCGGNAVDHMINEGVDGVEFICA
                     NTDSQALKRNKAKTLLQLGPNVTKGLGAGANPDIGREAAMEDRERIIELIEGADMLFL
                     TAGMGGGTGTGAAPIVAEAARELGILTVAVVTKPFAFEGKRQKIAQQGLEQLCRHVDS
                     LIVIPNDKLMQVLGEDVSMLDAFKAANTVLHGAVAGIAEVIKCPGLVNVDFADVRTVM
                     SEMGMAMMGSAVAAGIERAHSAAEQAVASPLLEDVNLAGARGVLVNITAGSSLKMKEV
                     HEVMNTIKGFTAEDATVIVGTVIDETMEDRLRVTMVATGLGGAVNRPQQKPLTMVKTG
                     TDGVPVEVNYGDLEAPAVMRRRSRDAAVAAMKQSGVDMLDIPAFLRKQAD"
     gene            10447..11358
                     /gene="lpxC"
                     /locus_tag="VN496_13585"
     CDS             10447..11358
                     /gene="lpxC"
                     /locus_tag="VN496_13585"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXF18035.1"
                     /translation="MIKQRTLKNIVRATGVGLHTGEKVYLSLRPAVANSGIIFRRIDL
                     DPVVEIKADPYAVGDTRLSSCLERDGARVQTVEHLMSALAGLGIDNLYVDLTSGEIPI
                     MDGSASPFVFLLQSAGIEEQPAAKKFIRIKKPVEVKQGDKWVRFDPYNGFRVDFSIDF
                     RHPVFAHSNKRVVVDFEHTSYIKEVSRARTFGFMHDVENMRAQGLALGGSLDNAIVMD
                     DYRVLNADGLRYEDEFVKHKALDAIGDLYLLGHPIIGAYSAYKSGHALNNALLRQLMD
                     DAACWEFVSFLKAEDAPAFLRMQMQVA"
     gene            complement(11402..11866)
                     /locus_tag="VN496_13590"
     CDS             complement(11402..11866)
                     /locus_tag="VN496_13590"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HXF18036.1"
                     /translation="MSDTRVGVLLEKLPELQHLKRQLTDLAALQRLLVDILPGNFSTS
                     VNVACIKGGELVLATDNGAVASKLRQIAPRTLDLLRRQGYEITGIDIQVQVRMRDKPL
                     PGKQLLLGPNACSSVDLLVQRLEASPLKDALKRLGCRGGPTATAKAATGAKT"
     gene            11893..12810
                     /locus_tag="VN496_13595"
     CDS             11893..12810
                     /locus_tag="VN496_13595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXF18037.1"
                     /translation="MNIILVSNKLTQARSFTLSAPQMVFLGLLFGLVIVSLAVLLNYF
                     SLRYAVTNQSPYLQSLLEAVQAEQTRKTQSYLRESLNTMAARLGEMQAKLLRLDTLGE
                     RLTKLAGIKPQEFMFEQTPGRGGAVSSIPAQEFSMNELLDKVNHLAIQVDDRTDKLGV
                     LESMLTLSHAKKQLVPTQLPVSTGWYSSNFGWRIDPFTGWNAFHEGMDFMAPEGTAIT
                     SAAGGVVVYSGLHPQYGNMIEIDHGNGLVTRYAHASKLIAKVGDVVLRGQKISEVGNT
                     GRSTGTHLHFEVRQWGAPQNPARFLQVPG"
     gene            12887..15607
                     /gene="secA"
                     /locus_tag="VN496_13600"
     CDS             12887..15607
                     /gene="secA"
                     /locus_tag="VN496_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXF18038.1"
                     /translation="MINSILKKIFGSRNDRLLRKYAHAVTAINLLEPQMQQLSDAELR
                     ARTDVFRQRATDGESLDDLLVEAFATVREASRRVLNMRHFDVQLLGGIALHNGKIAEM
                     RTGEGKTLVATLPAYLNTLSGKGMHVVTVNDYLASRDAEWMGKLYRFLGLSVGVNLSQ
                     MEHELKQQAYAADITYGTNSEFGFDYLRDNMVFQASERVQRGLTYAIVDEVDSILIDE
                     ARTPLIISGQAEDSTELYYRMNEIVPKLVRVQHDGGPGDFTVDEKAHQVLLTESGHER
                     AEELLTRAGLLPENGSLYDAANINLMHHLYAGLRAHSLFHRDQHYVAQNNEITIVDEF
                     TGRLTPGRRWSDGLHQAIEAKEGVRIQRENQTLASITYQNYFRMYRKLAGMTGTADTE
                     AYEFQQIYGLETVIMPTHMPMVREDRLDQVYRTAQEKYQAVIKDISNCYQNGQPVLVG
                     TTSIENSELLSKLLEKEKLPHQVLNAKQHAREAEIIAQAGRPKMVTIATNMAGRGTDI
                     VLGGNPEPEINKVRNDASLPEQGKETRVAEMREQWKKLHEDVIQAGGLHIVGTERHES
                     RRVDNQLRGRSGRQGDPGSSRFYLSLEDPLLRIFASDRVAAIMDRLKMPEGEAIEHPW
                     VTRAIENAQRKVEARNFDIRKQLLEYDDVANDQRKVIYQQRNELLEATDISDTIRSMR
                     EGAFDAVISVHIPPGSVDEQWDVPGLEKTLAGEFSLRLPIKEWLNNEADLDEEHLRAR
                     IISAASAYYEEKFASVEPQKVRDYERAIMLNFLDSHWREHLSALDHLRQGIHLRGYAQ
                     KNPKQEYKREAFELFSMMLDTVRAEVTRQLMIVQIQTAQEVEVAVEKQEQAELKNLQY
                     HHNVDQDEALEQANADVKVQPFIRGQKVGRNDPCPCGSGKKYKQCHGKLG"
     gene            15662..>16756
                     /gene="argJ"
                     /locus_tag="VN496_13605"
     CDS             15662..>16756
                     /gene="argJ"
                     /locus_tag="VN496_13605"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXF18039.1"
                     /translation="MAVNLATPNPSDLLAIHGIRLGVTEAGIRKPNRKDLLLITLAAG
                     TTVAGVFTRNRFCAAPVSVAKENLKRSKIRALIVNTGCANAGTGADGIERARQVCKNV
                     STLIGCNPEEVLPFSTGVIMESLPIDRIALGLPRCVDGLGAGNWAEAATAIMTTDTVA
                     KAFSRRTDIGGSMVSVTGIAKGSGMIRPNMATMLGFIATDADIEQNLLQQITKFAADR
                     TFNCVTVDGDTSTNDALVVMATGQNKSPRITKSDSTEYNLLRAAITEIASLLAQALVR
                     DGEGASKFITINVAGGRTETECRSVGYAIAHSPLVKTAFFASDPNLGRILAAIGNACI
                     DELDVSKVDLYLDDVNVAKSGERAQAYREED"
BASE COUNT         3942 a         4145 c         4904 g         3765 t
ORIGIN      
        1 aaacagcttc ccattcgggc cgcagattcc agagcgaatc aagcgggttc ggaaattgtc
       61 cgaacagaac ttaatgaaga tgtcggtttg ttgcgcctcg ctctgatcga ggccgtcatg
      121 aaagaagcgc gtgccgatcg aactgactaa taggcgagct ttggtgctcg gtctaggggc
      181 aagcggacta tcgatggccc gctggctgag caaacgcggg gccgtggttc gcgttgctga
      241 tagtcgtgat tcgccgccat tcgcctcaac cttgcgcgga gaactcccgg ctgtcgaact
      301 gcagacgggg gcatttcgct tcgagagcct ggatgatatc gaccttatgg cggtcagtcc
      361 tggagtgccc ctggccgagc cggtcattca acgagcagta gagcgaggag tcgaagtagt
      421 cggcgacata gagttgttcg cgagaatcag aagcgagttt cctggcagcc gggtgattgc
      481 cattaccggg tctaacggaa aaagcacagt aacggaaatg gtcggcgcga tggtacgtgc
      541 cgcaaagatc aggacactgg ttgccgggaa tatcggtttg cccattctgg atgcggtgtt
      601 ggaagtcgca gccggtagcc gtcgtcctcc ggatgtattc gtgcttgaac tgtctagctt
      661 ccaactggag tcaacgcgta ccttgaatcc cgatgtagcc gccgtgctga acctgagcga
      721 ggaccatttg gatcgctatt cgggcatgct cgattatgca acggcgaaat cacgcatatt
      781 catcggcgcc ggaatccagg tcataaacag acaagatgcg tgggcaaaga acatgatcgg
      841 cgggggacgt ccggttttga ccttcggctt ggatgagcct tgggtcgacg gcaattgggg
      901 acttcgtcaa atgaatgatg acctctggtt ggcggaaggc agagcgaatc tgatgaaggt
      961 ctccgaactc agagtagagg gcttgcacaa tgctggcaat gctttggccg ctttggcgct
     1021 atgccgcgca attggccttc cttacgagcc gattcttgat gctctcttca cgttcgaggg
     1081 tttgccacac cgggttcata aagttgcaga gatcaagggc atatctttct acgacgattc
     1141 caaggggact aatgtaggcg ctactgtagc cgctctcatg ggaatcgccg cgaaagttgt
     1201 gctgattgcg ggcggagagg gcaaagggca ggacttctcg ccgttacgca aggcggttac
     1261 cgagaaagca agaagtgtcg tgcttattgg acgagaccgc gacaagatcg ccgccgccct
     1321 ggaaggctct ggcgtcccaa tcataaatgc tgagaatatg cgcgacgcag tacgcgtcgg
     1381 attttcactt gcgcaaatcg gcgatgcggt gatgttgtct ccagcgtgcg ccagcttcga
     1441 catgttcagc aactacgaac atcgcgccaa ggtttttatc gacgaagtcc aggcattgga
     1501 gaccgggcct tgaacggatc cctgagcctc ggcgtcgccg caaaatccac ttcaattgcg
     1561 gaattcgatt acatcctcat cagtatcgcg ggccttctcc ttgccatggg attgatcatg
     1621 gtctattcat cttcggtcgc aatcgccgaa ggcggccgct ataccggcaa ccaggcaacc
     1681 tactacctcg tacgacacgg gatattcctg ttcgtcggaa tcgtggccgg cttcatatgc
     1741 ttccagttcc cattaaattt ctgggaacag gccgcaccct acttgttctg cgttggtgtc
     1801 gcgttgctga tggtcgttct tatcccaggg ctgggacgcg aagtaaatgg cagccaaaga
     1861 tggctttcgc ttggcctgtt caatctgcaa ccttcagaac taatgaaatt attcgcggtg
     1921 ttgtatgccg ccgactacac ggtccgcaaa gcagcactga tgcacagctt tcgcaagggc
     1981 tttttgccga tgctggttgt aatgcttctt acaggcgggt tgttgttgca agagcccgat
     2041 ttcggggcgt ttgccgtgat cagcgctatt gccatgggca tccttttttt gggcggcatg
     2101 aattggaaat tatttgcaac gttaatcttg cttctgctgt taggttttgt agttctgatc
     2161 tggacgtcgc catatcgcct gcagcgggtt accggattta tggacccatg gtcggatccc
     2221 ttcggcaagg ggtatcagtt gtcccacgcc ctcattgcct tcggccgggg agagtggttc
     2281 ggcgttgggc tgggcgcgag cgtagaaaag cttttttatt tgccggaagc gcatacggat
     2341 tttctgcttg ccgtcatagc cgaagaactg ggtttctgcg gggtcttaac ggtaatggtt
     2401 ttattcggcg taatgatatg gaaagcattc gctattggta atcaggcggc acgcatggag
     2461 cgctgcttcc cgggactggt agcccaggga atagccttgt ggcttggagt ccagacgttc
     2521 ataaacatgg gtgtgaacat gggggtactg ccgaccaaag gtttgacttt gcctctactg
     2581 tcattcggcg gcagcggcat cgtagccaat tgttgtgcgc ttgccgttct gctgcgagtc
     2641 gattatgaaa acaggaaaat gatgatgggc tattcgcttt gagtccaaca gtccgcagtt
     2701 ctttctgcaa ggcgattcta tattccgtca tcgccggtat gcgcaaccgc gacgcggcca
     2761 cgccgtcgct tcccggacca gccggccaca gggccgggca gtacaccgct cgggcggcca
     2821 catgagaacg atccttatca tggcgggtgg caccggaggc catatttttc cggcactcgc
     2881 ggttgccgat caaatgcgcg acagcgattg gagagtcgtt tggttgggct caaaaacggg
     2941 catggagtca aggctggttc cgcagtacgg ctttgaaatg gaatgggttc gtttctccgg
     3001 tctgcgcggc aaaggcattc tgcgcgcact gttgctgcca attaacttgt tgatcgcatt
     3061 cgcacagagc gcccgcgcta ttttccgggt gcggcccgat gtcgtacttg gtatgggagg
     3121 ctatatctcc tttcccggcg ggttgatggc cgcactgctg gcgcgtccat tgattttgca
     3181 tgagcaaaat tctgtagccg ggcttgccaa caaggtgctg gcacggttcg cagatcgcgt
     3241 cttggttgcc tttccgaatg cgttgcgcaa tgcggaatgg accggcaatc cggtgcgccg
     3301 tgacatcacg caattggtta cgcccgaacg gcggtacaga gaccgcactg gaaatctcaa
     3361 tgtgttggtt gtcggaggaa gcctcggcgc acaggttctg aatgaaactg tcccacgcgc
     3421 gctggcgctg atgccgccct caggcaggcc aaatgtgaca catcaggcgg gcgagcagca
     3481 tcttgctatg ttagagcggg aatatcggct tgctggagtt agtgctacag cgttggcgtt
     3541 tattgaggat atgtccagtg cctatgcatc ggcggacctg gtcatctgcc gagcgggcgc
     3601 tctgacgatt tccgagatcg ccgtcgcagg agttgcgagc gtgctggttc ctttcgcatt
     3661 cgcggtcgac gaccaccaga ccttcaacgc ccgctatcta tcggaccgct ccgcagcgct
     3721 tttgatttct caagccgact ttaccgctcg caagctggcc gacgtgctgc tccgctgtac
     3781 aagggcaaaa ctgctcgaga tggcgataaa agcccgtgaa ttgggcaagc cagatgcagc
     3841 gcaaattgtc gcgcaacgtt gtatggccat cgccgcatga aacacaaggt aaaacatgtc
     3901 cacttcatcg gaattggtgg cgcgggaatg agcggaatcg cagaagtgat gctcaacctg
     3961 ggattcgaag tcagcggttc tgatatttca gaaaatgccg ccacgctacg actcaagaac
     4021 cagggagcca aaatttcgat tggccatgat gcgagcaatg tgagcggagc gcatgccgta
     4081 gtgacgtcgg ccgcggtcag gcaggacaat ccagaagtgg ctgctgcccg cgcgaagcga
     4141 ataccggtcg tgccgagagc catgatgctg gcggagttaa tgcggttgaa gcagggcatt
     4201 gcaatcgccg gaacgcatgg caaaacgacc accaccagcc tcgtagccag cgtgctggcg
     4261 caggcaggaa tggatccaac cttcgttatc ggcggccgcc ttgaggccgc cggatcccac
     4321 gcgaagcttg ggaccggcga gttcatcgtg gtggaggccg atgaatcaga tgcctccttc
     4381 ctttatttgc agccagttct tgcggtcgtc acgaatattg acgcagacca catggaaact
     4441 tacgggcatg actttggccg cctgcggcat gcgtttctgg aatttatcga acacttgcct
     4501 ttttacggaa tggcagttct ttgcgcggat gacgccaatg tcaaaagctt gttgccgatt
     4561 atcaccaagc aagtgacgac atatggtatc gacgaaggtg cacaggttcg tgccgtggag
     4621 attcatgccg acgccgcccg catgtgcttc cgtgtggtac gtagaaacgg caatttgaaa
     4681 cctgccgctg atctgaacat cgtgctgaac ctgcctggtt tacataacgt tcgtaatgcg
     4741 cttgccgcaa ttgcagtcgg aatggaaatt ggcgcttcag acgaagctat cgtaaaagcg
     4801 ctcgccgaat ttcgcggcgt cgggaggcgt tttcagcgct atggcgaggt ccccctcccc
     4861 caaggaagtt tcacgctcat tgatgactat ggccatcatc cggcggagat ggccgcgaca
     4921 atttctgctg cgcgcggcgc tttcccgaac cggcgactag tgctggcctt ccaacctcac
     4981 cgctacacgc gaacgcggga ttgtttcgag gattttgtca aggttctgtc gtccgtcgac
     5041 gcgctcttgc ttaccgaagt ctatccggcg ggggaagcgc cgatcgtggc tgcggatggt
     5101 cgcgcgctag cgcgtgccgt tcgtgtagcc ggaaaaatcg aaccggtatt cgtggaacaa
     5161 attgtggatc ttccggccgc gatcctgacg aaaacaacca gcggtgacgt agtcatcacg
     5221 atgggagccg gatccattgg aacggtgccc ggaaaacttg caaaaggaaa ttgagttaga
     5281 agcatggaca tgacggacct tatacagata gagacggcgg gactgcgagg cgtatacagg
     5341 cgcaacgagc cgatgcatac gcacgtcact tggcgagccg gcggaatggc tgatcgcgcc
     5401 tacacgcctg ccgacctcga cgatctcggc ttgtttttgc gccgtctgcc gcaacaggag
     5461 ccaatattgt tcgttggcct tggcagcaat ttgttgatcc gggatggagg ttttcgcggg
     5521 acagtcgtgt tcacacatcg cgcacttgca catcttgctc ttgagagcga ttccaatccg
     5581 cctgaagttt atgcggaagc gggtgttgcg acaccgaaag tcgcgcgttt cgccgcaaat
     5641 aacaacttgg aacatgcgga atttttggcg ggcattcccg gtactgttgg cggtgtattg
     5701 gcgatgaatg ccggctgcta tggatccgaa acttgggatg tggtcacgcg ggtgcgaacc
     5761 atggaccgcg ccgggaagtt gcatgttaga gcacgacgcg aatatgaagt tgcttatcgc
     5821 agtgtatcgc cgctgagcgc gcccgacgaa ttctttgtcg ccgcatggtt tggctttgct
     5881 cgaggggatg gagagaaagc gcgtcaacgg atcagggaat tgctggccaa gcgaatcgcc
     5941 acgcaaccgc tgggtgaacc gaatgcaggc tcggtctttc gcaatccacc gcacgactat
     6001 gctgcgcgtt tgatcgaggc aagcggcctc aaaggatggc gcataggtgc agctgaagtg
     6061 tcttcagtcc acgcaaattt catcgtcaat aaaggcgggg cgattgcccg ggacatggag
     6121 caattgattt ctcttgttca gaacaccgta aaacacgaaa ccggggtaac gctggagcct
     6181 gaagtacgga tagttggtga agcatggaag tgaaagcatg aacatcaaga ttggaaaggt
     6241 ggccgtgttg atgggtggcc gctcatccga aagagagata tcgctgctat ccggacaggc
     6301 ggtgctgggc gcattgcgtc agcgcggcgt ggatgcccat gctttcgacc cgaaagagcg
     6361 tgaattaggc gatctcaagc gagaaaaatt cgaacgcgtt ttcatcgccc tgcatggtcg
     6421 ctacggagaa gacggtaccg ttcagggtgc actcgaactg cttggcattc cctatagcgg
     6481 gagcggcgta atggcgagcg ctctcgcaat ggacaaatgg cgcactaagc gcgtttggct
     6541 agccgagggg attcctgtcc ccagcgattt actggtgaat gaatcctcag attgggatgc
     6601 cgtcgcaaag acgctggggt tgccgctcat catcaagccg gttcacgagg gatcaaccat
     6661 tggcctgacc aaagttctca cgattaaaga attgcccgct gcgtacgcgc tggcggtgaa
     6721 gcatgaccgc atggtacttg cagaagaatt cgtggatggt cgggaattga ctgcatcgat
     6781 cctgcgagaa accgctctgc cattgatacg catcgaggca ccgcaaggaa attacgatta
     6841 tcagaataaa tattttagcg ataagaccaa atatcactgt ccggccggga tcgcatcgga
     6901 gctggaaaaa accattctgg aagttgcgct gcgcgcgttt aacgttctcg gatgtgaagg
     6961 gtggggccgc gccgatctaa tcctgcgagc cgacaatacc ttctcctttt tggagatgaa
     7021 cacttctcct ggaatgacga gccatagtct ggtcccgatg gcagcgcgac aagcgggcat
     7081 cgcgtttcct gaccttgtgc tccggattct cgaggctgcg catgtgggat gacgttgcga
     7141 cattgaggct cagttcaaat gttctcttcg ccctggctgc agcactggtg ctctacggtg
     7201 cggtggatta tgtagtccac ctaaagatct atcccctgcg ggatgtccgc gttacgggag
     7261 atatctcgca cgtgacacgc gaacaagtcg aagccgtttt gtctcgtgaa ctgcgcggca
     7321 atttctttac ggtcgacctt gcgcacgcac gccaggcttt taaaaaattg ccgtgggtac
     7381 gtgcggtaaa tgtgcgccgc cagtggccgg accgacttga cttcgaggtc gaggaatata
     7441 gaccaattgc gcggtggggc tcaactgcac tcgtcagcgc agaaggcgag gtgttcgaag
     7501 ctgcggtcaa ctcgaggttg ccggtactgc agggaccgga tggaacggcg gcggaaatcg
     7561 tgacgcggct ccagacgttt gggaaggtgt tggaaccgtc aggacgacga gtagcgcgaa
     7621 tcaccctgtc ggcgcgacgt gcgtgggttc tggaatttga tgatggcatg gtgcttgagc
     7681 ttgggcgcga caacctggaa gccaggcttt ccggctttat atcagcgtac ggacaaacgg
     7741 tagctcgtct gcccgaacgc gcggcgtatg tggaccttcg ctacgcgaat ggatttgcgg
     7801 tgcgtgccgt gggcctcaag tcgaataaga aaaagagtta aaggaaaaat ggcacttagc
     7861 ggaagaaaag aaaacaagaa tcttatcgtt ggattggata tcgggacatc gaagatcgct
     7921 gccatcgtag ctgagatgaa aactgaagga gggttcgaag tcgttggcat gggaagttcg
     7981 ccgtcgcgtg gattgaaaaa gggtgtggtc gtcaatattg agtctaccgt caatgcgatt
     8041 caacgggcgc tcgaagaagc cgagctgatg gctgattgca agatacgtga tgtctggacg
     8101 ggcatcgcag gcagtcatat tcggagtttc aattctcatg gcatggtggc aatcaaggac
     8161 cgcgaggtcg cgcaaagcga tgtggaccgc gtcgtggaga cggccaaagc aatacctatc
     8221 cctacggatc agcagatcct tcacgtactg aatcaggaat tcgtgattga cggccaggag
     8281 gatgtccgcg agccgcttgg aatgagcgga gtacggctcg aggtgaaagt gcacattgtg
     8341 accggtgcag tttccgccgc tcaaaacatc atgaaatgcg taaggcgttg cggcctcgaa
     8401 gtcaatgatc tcatccttca accattggcg tcatccatgg ctgtcctttc cgaggacgag
     8461 aaagatctcg gtgtgtgcct cgttgacatc ggtggcggaa catcggacat cgccgtgttc
     8521 acgcaaggag cgataaggca tacggctgta attccgatcg ccggagacca gattacgaac
     8581 gatatagcga tggcgttgcg gacgccgaca aaggaggccg aggagataaa gcgacgctat
     8641 ggatgcgcgc tacgcgaact cgccgatccg caagaaatgg ttgaggtgcc gggcgttggc
     8701 gagcgtggct ccaagaaaat gtcacgtcag acattagctg aagtcgttga gcctcgcgtc
     8761 gaggaactct actcgctggt gcaggccgag ctgcgccgca gtggcttcga agaactgctt
     8821 tcctctggca tcgtcattac cggcggcagt gccggtatgc agggaatggt ggagcttggc
     8881 gaagaagttt ttcacatgcc tgtgcgtctc ggattgccaa gctacgcagg tggactagcc
     8941 gaagtaatcc gcaatccgcg ctattcgaca ggcgtaggac ttttgatggc gggcatcagc
     9001 cagcatcagc ggcatcagat cgaaaaactg caaagtggat caatcaagca aattgtaggg
     9061 cgcatgaaaa aatggttcga gacaaatttt tgaagggaag tgtcaggaag aagttcagtt
     9121 tttacgtttt ataactacta caaggagcaa ctgatgtttg aaattgtaga tacgcaacct
     9181 caggaagcag taatcaaggt cgtcggcgtt ggtgggtgcg gtggcaacgc ggtagatcac
     9241 atgattaatg aaggagtgga tggcgtggaa ttcatatgcg ccaatacaga ttcccaggcg
     9301 ttgaagcgga acaaggccaa aacgctcctt caactgggcc ccaacgtaac caagggatta
     9361 ggggcgggtg cgaatccgga catcgggcgg gaagcggcaa tggaagaccg ggaacgcatc
     9421 atcgaactta tcgaaggcgc cgatatgttg ttccttacgg cgggcatggg tggcggaacc
     9481 ggcacgggcg cggcaccaat tgtggcggaa gcagcacgtg aactcggaat tttaacggtt
     9541 gccgttgtaa ccaagccctt tgcgtttgag ggaaaacgtc aaaagatcgc gcagcaagga
     9601 ctggaacaac tttgccgtca cgtggattcg cttatagtga ttccaaacga caaactcatg
     9661 caggttctgg gtgaagacgt cagcatgctg gatgcattca aagcagcaaa taccgttttg
     9721 cacggtgcag ttgccggcat tgcagaggtc atcaaatgcc caggattggt caatgtggat
     9781 tttgccgatg ttcgcacggt catgtcagag atgggcatgg cgatgatggg ctcggcagtg
     9841 gccgctggaa tagaaagagc acattcagcg gctgagcaag ctgtcgcgag cccgctattg
     9901 gaagatgtaa atctagctgg ggctcgcggc gtactcgtaa atattacggc tggttcaagc
     9961 ctcaaaatga aggaagttca tgaagtcatg aacaccatca aaggatttac ggcggaagat
    10021 gcgacggtga ttgtcggtac tgtcattgat gaaacaatgg aagatcgctt gcgggtcacc
    10081 atggtagcta ccgggttagg tggcgcagtc aaccggccgc agcaaaaacc gttgacaatg
    10141 gtgaagactg gaaccgacgg cgtaccggtg gaagtaaact acggagatct ggaggctccg
    10201 gccgtaatgc gccggcgctc gcgagacgct gctgtggccg ccatgaaaca gtccggagtc
    10261 gacatgctgg atattccggc tttcttgcgc aagcaagctg attagaggaa ttgggcttga
    10321 gtgctgcggc tgcgacgcaa cgccttttgt tgtggcgcag cgcaaatgcc tatgataatc
    10381 taatcagtcg cttagtcctt tgatagcacc aaagcgtaaa ccatcaatat tgctcgagcg
    10441 catggcatga tcaaacaacg cacgctgaag aacatcgttc gagcaacagg ggttggctta
    10501 catacaggcg agaaggttta tctgtcctta cggccggcag tcgccaacag cggcattatc
    10561 tttcgccgca tcgacctcga cccagtagtc gagatcaagg cagatccgta cgcggtcggg
    10621 gatacccgtt tatcgtcttg cctggagcgg gatggtgcgc gcgtccagac tgtagagcat
    10681 ttgatgtcag ctttggccgg actgggcatc gataatctct acgtcgactt gacctcgggc
    10741 gagataccga taatggacgg atcggcgtcc ccttttgttt ttctcttgca atctgcgggg
    10801 atcgaagagc agccggcagc caaaaagttc attcgcatca aaaaaccagt cgaagtaaag
    10861 cagggcgaca agtgggtgag attcgatcct tacaatggat ttagagtcga tttcagcatc
    10921 gatttccggc atccggtatt cgctcattcg aacaagcgtg tcgttgttga tttcgagcat
    10981 acttcgtata taaaagaagt aagccgcgcc aggacattcg gctttatgca cgacgttgaa
    11041 aatatgcgtg cgcaagggct ggctcttgga ggcagcctgg ataatgccat cgtgatggat
    11101 gactatcggg tcttaaacgc agatggtttg cgttacgagg atgaatttgt gaagcacaag
    11161 gcgctggatg cgattggcga cctgtacttg ctgggtcacc caatcatagg ggcctattcc
    11221 gcgtacaaat caggccatgc tttgaataat gcattgttgc gccagttgat ggacgacgcc
    11281 gcgtgttggg agttcgttag cttcctgaaa gcggaggacg ctcccgcatt cttgcgcatg
    11341 cagatgcagg ttgcctaggg caaccctgta acgacctcct gaagcgcgtc cagcgatctt
    11401 cctaagtttt cgcccctgtt gcagccttgg ccgtagcagt tggtccgccg cggcacccca
    11461 accgcttcaa agcatccttc aaaggtgagg cctcaaggcg ctgaaccagc aaatcgactg
    11521 aggaacaggc attgggaccg agcaaaagtt gttttccggg caatggctta tcgcgcattc
    11581 ttacttgcac ttgtatgtca attccagtaa tctcataacc ctgtcggcgt aacaaatcca
    11641 aagttcgggg agcgatctgc ctcaatttgg aagcgacagc tccgttatcg gtggcaagaa
    11701 ctaactcgcc gcctttaatg catgcaacgt ttacgctagt agaaaagttg cccggcagaa
    11761 tatcgaccag gagcctttgg agggcggcca ggtccgttaa ctgccgtttc agatgctgca
    11821 actcgggaag tttctctagc agtacgccaa cgcgcgtatc ggacattgtt tcacgagaca
    11881 tgaggggaac agatgaacat tattctagtc tccaacaagt tgacccaggc tcgcagtttt
    11941 acattaagcg cgcctcaaat ggttttcctg ggcttgctat ttggcttggt cattgtctcg
    12001 ctggcggtgc tgctcaatta tttctcgctt cgttacgctg tgaccaatca aagtccatat
    12061 cttcagtcgt tactggaggc tgtacaggcg gagcagaccc ggaagaccca atcttatcta
    12121 cgagaaagcc ttaatactat ggcggcgcgg ctcggggaga tgcaagccaa actgttgcgg
    12181 cttgataccc tcggggaacg gctcaccaag ttggctggta tcaaacctca agaattcatg
    12241 ttcgagcaga caccggggcg cggcggtgcg gtgtcgtcca ttccagcaca agagttttcc
    12301 atgaatgaac tgctcgacaa ggtcaatcat ctggcaatac aagtcgacga tcgcactgac
    12361 aagctgggag tgcttgaatc catgctgacg ctgtctcatg ccaagaagca gctggtaccg
    12421 acccagttgc cggtgtcaac cggttggtac tcttcaaatt ttggctggcg gattgatcct
    12481 tttacgggat ggaatgcgtt ccacgagggc atggatttta tggctccgga aggcacggca
    12541 attacatccg ctgctggagg cgtagtggtg tactcgggcc ttcaccctca atatggtaat
    12601 atgatcgaaa tagaccacgg caacggtctc gtcacacgat acgcgcacgc atcgaagctc
    12661 attgcgaaag tcggagatgt ggtcctgcgc gggcaaaaga tatcagaagt gggtaatacc
    12721 ggccgttcaa ccggaacgca tttgcatttc gaagtgcgcc aatggggagc tccacagaac
    12781 ccggcgcgct ttcttcaggt gccgggttga actgaaaaaa aagtaatttc cagagagggg
    12841 tgggggcggg cgtccccacc cctcttgctt taaggacatc ggaagcatga tcaacagcat
    12901 tctcaaaaaa atatttggaa gccggaatga tcgcctgttg cggaagtacg cgcatgcggt
    12961 cactgccatc aatttgctag agccgcaaat gcagcagttg agtgatgcag aacttcgtgc
    13021 acgcactgat gtattcagac aaagggcaac cgatggcgaa tcgctggacg atttgctggt
    13081 tgaggccttt gccacggtac gcgaagcgag tcgacgtgtc ctgaacatgc ggcatttcga
    13141 cgtgcagttg cttggcggta tagccttgca caacggcaaa atcgccgaaa tgcgtactgg
    13201 ggaaggcaag actcttgtcg cgactttgcc cgcctacctg aacaccctgt cgggcaaagg
    13261 catgcatgtc gtcaccgtca acgattatct tgcgagccgg gatgcagagt ggatgggaaa
    13321 actatatcgt tttctcggtt tatcggtggg cgtaaatctc tcacaaatgg agcatgaact
    13381 gaagcagcaa gcctacgctg cggacattac gtacggaacg aacagtgaat tcggattcga
    13441 ctatctgcgg gacaacatgg tttttcaagc gtctgaacgg gttcagcgcg ggcttactta
    13501 cgcaatcgtc gatgaggtgg attccatctt gattgatgag gcgcggacgc cactgatcat
    13561 ttccggacag gccgaggata gtactgaact ctattaccgc atgaacgaaa ttgttcccaa
    13621 gctggttcgt gtgcagcatg acggcgggcc cggggatttc acggttgatg aaaaagcaca
    13681 tcaagttttg cttacggaat ccggacacga gcgggccgaa gagctgctga cgcgcgccgg
    13741 attattgcct gaaaatggca gcctgtacga tgctgcaaat atcaatttga tgcatcattt
    13801 gtatgctggt ttgcgtgcgc attccttgtt ccatcgcgat caacactatg ttgctcaaaa
    13861 caacgaaatc actatagtcg atgaatttac cggccggcta acgcctggtc ggcgatggtc
    13921 ggacggattg catcaggcga tcgaggccaa agaaggtgta cgcatacagc gggaaaatca
    13981 gacgttggcc tcgattacct accagaatta tttccggatg taccggaaac tcgcaggaat
    14041 gaccggcaca gcggatacgg aagcctacga gtttcagcag atttacggac tcgagacggt
    14101 catcatgccg acacacatgc ccatggtccg tgaagaccgt ctcgaccagg tctatcgtac
    14161 ggcacaggaa aagtaccagg cggtcataaa ggacataagt aattgctatc agaatggtca
    14221 gcccgttttg gtcggcacga catcgattga gaattccgaa ctcctgtcca agctgctgga
    14281 aaaggagaag cttccccatc aggtcttaaa tgcaaaacag cacgcgcgag aagctgaaat
    14341 aatcgcacag gctggccgtc caaaaatggt tactatcgcg acgaatatgg ctggtcgtgg
    14401 cactgacata gtactgggcg gaaatcccga gcctgagatc aacaaggtcc gtaatgacgc
    14461 aagtctgcct gagcagggaa aagaaacgcg cgtagcggaa atgcgtgagc aatggaaaaa
    14521 actgcatgaa gacgtcattc aggccggtgg gcttcacatt gttggcacgg agcgccatga
    14581 gtcccggcga gtcgataacc aactgcgcgg tcgatccggg cgccagggag atccaggctc
    14641 aagccgattt tatctttcgc tggaagaccc gctactgagg atatttgcct cggatcgtgt
    14701 ggcagcaatc atggaccgtc tcaaaatgcc ggagggcgag gccattgagc atccctgggt
    14761 tacacgagca atagagaatg cacagaggaa ggtggaggcg cgtaatttcg atattcgcaa
    14821 gcaattgctt gaatacgacg atgtggcaaa cgatcagcgc aaagtgattt accagcaacg
    14881 aaatgaatta ctggaggcga cggatatctc ggataccatc cgttcaatgc gagagggcgc
    14941 ctttgatgca gtgatttctg tccatattcc gcctggaagt gtagatgaac aatgggacgt
    15001 gccgggtctc gaaaagactc ttgccgggga attcagtttg cggctgccga ttaaggaatg
    15061 gttaaacaat gaagctgatc tcgatgaaga acatctccgc gccaggatca taagcgcggc
    15121 cagcgcatat tacgaagaaa aatttgccag tgttgagccg caaaaggtac gtgattacga
    15181 gcgcgcaatc atgctcaatt ttttggacag tcattggcgc gagcatctct ctgcactcga
    15241 tcacctgcgg cagggtatcc acttgcgtgg atatgcacag aagaatccca agcaagaata
    15301 caaacgtgag gctttcgagt tgttttcgat gatgctggat actgttcgag ccgaggttac
    15361 gcgccaatta atgatcgtgc agattcagac ggcgcaagag gtagaagtcg ccgttgagaa
    15421 acaagaacaa gcagaactaa aaaacctgca gtaccaccac aatgtcgacc aggacgaggc
    15481 gctcgaacaa gccaatgccg atgtaaaggt gcaacctttc attcgtggtc aaaaggtagg
    15541 gcgaaatgat ccttgcccct gtggttccgg caaaaagtac aaacagtgcc acgggaaact
    15601 gggctgatcc agtgttagtc gcgcttggtt tacctgatga aaaaaagcac gggctttgcc
    15661 gatggcggta aaccttgcaa ctcccaatcc atctgacctc ttagcgatcc acggcatacg
    15721 acttggcgta actgaagctg gaattcgcaa gcccaaccgt aaggacctgc tattgataac
    15781 gctggcggct ggaaccacgg tggccggagt atttacgcga aatcgttttt gcgcggcccc
    15841 tgtctctgtt gctaaggaaa acttgaagcg ttccaaaatc agggcgctaa tagttaatac
    15901 cggatgtgcc aatgctggca ccggcgccga tgggatcgag agagccaggc aagtatgcaa
    15961 aaacgtatcg acgttgattg gctgcaaccc ggaggaagtt ttgccatttt ccaccggggt
    16021 catcatggaa tccctgccca ttgaccgaat cgctctcggc ctcccgcggt gcgtcgacgg
    16081 attgggcgcc ggcaattggg cggaagctgc gacggcgata atgaccactg atactgtcgc
    16141 aaaagcgttt tccagacgga ccgatatcgg cggcagcatg gtttcagtca ctggtatcgc
    16201 caaaggttcc gggatgattc ggcccaacat ggccaccatg ctggggttca tagcaactga
    16261 tgcagatatt gaacaaaacc tgctgcagca aataacaaag ttcgcagcgg accgtacgtt
    16321 caattgcgtg acagttgatg gcgacacgtc caccaacgac gctttggttg tgatggccac
    16381 cggacaaaat aaatcaccac gtataacgaa atcggactcg accgaatata atttgctacg
    16441 cgccgcaata acagaaatcg cgtccctgct cgcgcaggca cttgtgcgag atggagaagg
    16501 tgcgtccaag ttcataacga taaatgtcgc tggaggaagg actgaaacgg aatgccgtag
    16561 cgttggctac gcaatagccc attcaccgct tgtcaagaca gctttttttg catccgaccc
    16621 taacctaggg cgcattcttg ctgcaatcgg aaacgcttgc attgatgagt tggatgtttc
    16681 gaaagtagat ctctatctgg atgacgtaaa cgtcgcaaaa tccggggagc gggcgcaggc
    16741 ttatcgagaa gaagat
//
LOCUS       DATMZQ010000427         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3449416, whole genome shotgun sequence.
ACCESSION   DATMZQ010000427 DATMZQ010000000
VERSION     DATMZQ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449416"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..336)
                     /locus_tag="VN496_13610"
     CDS             complement(55..336)
                     /locus_tag="VN496_13610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXF18040.1"
                     /translation="MAEVGTKLIFENDRVRVWEFTLRPGEGIEAHKHDNDYFFYPIEG
                     ATLDVTRASGTTRLTLKTGEVYFRKGGDTHAARNIDDHRYHEVLVELKA"
     gene            453..1529
                     /locus_tag="VN496_13615"
     CDS             453..1529
                     /locus_tag="VN496_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXF18041.1"
                     /translation="MSALEEKTFHLLILAVSIAFALVLQPLYGAIVWGSILAIVFAPL
                     YRRMLHVMRQKRSLAAAVTVALVLVLVILPLIIVVALLVREVTGAYSRIESGELDIVG
                     FFRRMFDLLPSWMADLLERFGLTDFGAVWERSIGTLLKGSQFVAAQAFVLGHNMLDFG
                     VSLFVMLYLLFFLLRDGQELSLRIRNAIPLPADQKVALISRFTIVIRATVKGNIFVAA
                     LQGALGGLIFWVMGIHAAVLWAVVMAFLSLLPAIGAALVWLPVAIYMVMTGDIWQGIG
                     LTAYGVLVIGLVDNFLRPILVGKDTKLPDYVVLISTLGGLTFFGINGFVLGPVIAAMF
                     MAVWSIFGASRQRAEEEHSASGNG"
     gene            complement(1633..3111)
                     /locus_tag="VN496_13620"
     CDS             complement(1633..3111)
                     /locus_tag="VN496_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXF18042.1"
                     /translation="MNTVDQTHRARTDVRRFADRAFSRAAGAALIEGNSIRLLRDAAQ
                     NYPAWLAAIGAARHHILFENYIITEDTVGERFAEAFIAKARDGVSVRLIYDWMGSFGR
                     ASSRFWNRLQTGGVQVRCYNPPRFDSPFGWLSRDHRKMLAVDGEVGFITGLCVGKSWE
                     GVPEKNLEPWRDTGVQVRGPAVAEIESAFEQVWAMTGGEDAFKRDASTDNRALAESGD
                     VAMRIVASAPATAGMFRLDQLIAALARERLWMTDAYYGGTPSYVEGLRAAARDGVDVR
                     LLVPNATDIPLLKPLSRSGYRPLLEGGVRLFEWNGTMLHAKTAVADGRWARVGSTNLN
                     IASWFGNCELDAVVEDEAFAHQMEQMYIEDLANSTEVVLDARRRVRAPGEPHHVQRRL
                     ASGRGSTGRAAAGAVRLGNAVGAAMINRRALEPVETRMLIPAALLLFALSVLLARFPL
                     LLIYPLLFILVWLATALLYKGFRAYRAARRARSGQTGERTAA"
     gene            complement(3209..>3609)
                     /locus_tag="VN496_13625"
     CDS             complement(3209..>3609)
                     /locus_tag="VN496_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HXF18043.1"
                     /translation="VYGTRTTGAENDIEQATNLARNMVTRWGMSDALGMVQLAPRQNP
                     YLPGALGADKPFSEETARVVDAEVLRIINECHEDALRLLREHRKPLNALVQALLVGDT
                     LGEQEILAVTGLPPAPALETQPLAVAAQQA"
BASE COUNT          662 a         1104 c         1060 g          783 t
ORIGIN      
        1 taatccggca gcgcgcgctg gtacggcgac agcatcaagt gacgctgtca gcaatcaggc
       61 tttgagctcg accaatactt catgatagcg atgatcgtcg atgttccgtg ccgcgtgcgt
      121 atcgccgcct ttgcggaaat aaacctcacc ggtcttcaag gtcagtcgcg tggtcccgct
      181 agcgcgtgta acgtccagcg tggcgccctc gatcgggtaa aagaaatagt cgttgtcgtg
      241 cttatgcgct tctatgcctt cccccggtcg cagcgtgaac tcccataccc gcacccgatc
      301 attttcgaat atcagtttgg tcccgacctc tgccataaac atttcctccg ttgcgatgcg
      361 agcgtgcggc actgacgcct gagccaaact gtccattggc ctaccgtgcg ttcagcgtac
      421 actggcatca ccgaacacac tatcgcggca tcgtgagcgc actcgaagaa aaaacgtttc
      481 acctgctcat tcttgcggta tcaattgcgt ttgcgctggt tttgcagccg ctctacggcg
      541 ccattgtttg gggcagcatt ctggcgatcg tgtttgcgcc attgtatcgg cggatgttgc
      601 acgtcatgcg gcaaaagcgc tcgctggcgg ccgctgtcac tgtggcactg gtgctcgtcc
      661 tcgttatcct gccattgatt atcgtcgtgg cgttgctggt gcgcgaggtg accggcgcct
      721 actcccggat tgaaagcggg gaactggaca tcgtcggctt cttccgccgc atgttcgatc
      781 ttttgccttc atggatggcg gatctgctgg agcgcttcgg attgaccgat ttcggcgctg
      841 tatgggagcg atccattggg accctgctca aaggcagcca gttcgtggcg gcacaagcgt
      901 ttgtgcttgg ccataacatg ctggacttcg gtgtcagctt gtttgtcatg ttgtatctgc
      961 ttttttttct tttgcgcgat ggccaagaac tgtccctgcg tatccggaat gccattccat
     1021 taccggcaga ccagaaggtc gcgctcataa gccggttcac tatcgtcatc cgcgctacgg
     1081 taaagggcaa catattcgtg gccgcgctgc agggtgcgct cggcggactg atattctggg
     1141 tgatgggtat ccatgcggcg gtattgtggg cggtggtcat ggcgttcctg tctttgctgc
     1201 ccgccatagg cgcggcactg gtctggttgc cggtggcgat ctatatggtc atgaccggtg
     1261 acatctggca gggaatcggg ctgaccgcgt acggagttct ggtcatcggc ctggtcgaca
     1321 acttcctgcg cccaattctg gtcggcaagg acaccaaatt gccagactat gtggtgctga
     1381 tctccacgct gggcggcctt acgtttttcg gcattaacgg cttcgtgctg gggccggtta
     1441 tcgcggccat gttcatggca gtgtggtcga tattcggcgc atcacggcag cgtgctgaag
     1501 aagagcactc cgcttctggt aacgggtgaa attttgacgt ccgccgaact cactgctgcg
     1561 cggtcttgaa atccaacgta aaacgcacgc atggattgaa gccgtcacca ccggccatat
     1621 cggcctgcgg actcaggcag cggttctctc gcccgtctgg ccggaccgtg cacgacgggc
     1681 cgcacggtat gccctgaagc ccttgtacaa cagcgcggtc gcaagccaaa ccaatatgaa
     1741 cagcagcgga tagatcagca acaacgggaa gcgcgccaac aacacgctca aggcgaacag
     1801 caaaagtgcg gcaggaatca gcatgcgcgt ctcgacgggc tccagcgcgc gtcggttgat
     1861 catggcggcg ccgacggcgt tgccgaggcg taccgcgccg gccgccgcac gccccgtact
     1921 gccgcgtccc gaggcaagcc ggcgttgtac atgatgcggc tcgccgggcg cgcgcacgcg
     1981 ccggcgcgcg tcgaggacga cttcagtgga gttcgccaga tcttcgatat acatctgctc
     2041 catctggtgc gcgaacgctt catcttcgac cacggcatcc aattcgcagt taccgaacca
     2101 gctcgctatg ttcaagttgg tggagccgac ccgcgcccaa cggccgtccg caaccgctgt
     2161 cttcgcatgc aacatcgtgc cgttccattc gaacaaacgc acgccgcctt ccagcagcgg
     2221 gcgatagccg gatctggaaa gcggtttcag cagagggatg tccgtcgcat tcggcaccag
     2281 caagcgcacg tcaacgccgt ccctggcggc ggctcgcaag ccttccacgt atgatggcgt
     2341 gccaccgtaa tatgcgtcgg tcatccacaa tcgctcgcgt gccagcgcgg cgataagctg
     2401 atccaaacga aacatgccgg ccgttgccgg tgcgctggcg acgatgcgca ttgctacatc
     2461 ccctgattcg gcaagcgccc ggttgtccgt cgatgcgtcg cgtttgaatg cgtcctcgcc
     2521 gccggtcatg gcccagactt gttcaaatgc cgattcgatt tcggcaactg ccggcccacg
     2581 cacctgcacg ccggtatcgc gccaaggctc cagattcttt tcgggtactc cttcccatga
     2641 tttgcccaca cacaggccag tgatgaaacc aacttcgcca tccaccgcca gcatcttgcg
     2701 gtgatcgcgc gagagccagc caaacggcga gtcgaatcgc ggcgggttat agcagcgcac
     2761 ttgcacaccg ccagtttgca ggcgattcca gaaacgactc gaggctcggc caaaactgcc
     2821 catccagtca taaatgagac gcacgctgac gccgtcacgg gctttggcta tgaacgcctc
     2881 ggcgaaccgt tctccgaccg tgtcctctgt gatgatgtag ttctcgaaca gaatgtggtg
     2941 ccgtgccgcg ccgatcgccg ccagccaggc ggggtagttt tgcgcagcat ccctgagcag
     3001 ccggatgctg ttgccctcga tcaacgcagc cccggcagcg cgtgaaaacg cgcggtcggc
     3061 gaaccggcgc acatccgtgc gggctctgtg ggtctggtcg actgtattca ttcggaatat
     3121 ttgtccgcgc gatattagcc cgtatgttaa tccagacaag ccattgcgcg gtctcgcaac
     3181 gccagcgatt gcggccgata cgccaaactc acgcctgttg agcggcaacc gcgagcggtt
     3241 gcgtctccag agccggggcg gggggaagtc cggtcacagc caggatctcc tgttcgccaa
     3301 gcgtatcgcc gaccagcagc gcctgcacta aggcgttaag tggcttgcga tgctcgcgca
     3361 gcaggcgcag ggcgtcctcg tggcattcat taatgatcct gagcacttca gcgtccacta
     3421 cgcgcgcggt ctcttcgctg aatggcttgt ctgcgccgag cgcaccggga agatatgggt
     3481 tctggcgggg tgcaagctgc accatgccga gtgcatcgct cataccccag cgcgtgacca
     3541 tattgcgggc aagattcgtt gcctgctcga tgtcgttctc cgctccggtt gtcctggttc
     3601 catacacga
//
LOCUS       DATMZQ010000428        10244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4141512, whole genome shotgun sequence.
ACCESSION   DATMZQ010000428 DATMZQ010000000
VERSION     DATMZQ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4141512"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VN496_13630"
     CDS             <1..852
                     /locus_tag="VN496_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXF18044.1"
                     /translation="WGYSWITSKIGLDYASPMDVVGLRLELGILTLFIGLIWTGRPLK
                     PQHWQALLMIGLIQTGAFLLLNTWALSEGGPGKTSVLVFTMPFWVLVFAWPMLQERIQ
                     GLQWVAVALAILGLLFVLEPWQMHTSLFSKTLAVIAGMCWAIGVVYAKRLHNHAKVEP
                     LAFTFWQMIIGLMPVIACQLMFERPPIVWSNTFVAVTMFNGVMATGIGWLAWLYVLHR
                     LPAGTTSLSSLGIPVIAIISSWIQLGEQPGRSELVGMLLIGAALALLSYLSIRRHEEP
                     EPMTGQE"
     gene            1022..1885
                     /locus_tag="VN496_13635"
     CDS             1022..1885
                     /locus_tag="VN496_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF18045.1"
                     /translation="MKPSSSTYLSIRGLRYHVRTWGDARAPKMFMLHGWMDVSASFQF
                     VVDELRHDWHVIAPDWRGFGLSEWSREGYWFPDYIADLDLLLEHFQPESPAYVVGHSM
                     GGNVANLYAGIRPERIGKLVLAEGFGLPPTKPENAPLRYAKWLEENRDPPTLRPYTSF
                     EEVAERLKKNNSRLSDERARFLAPHWAARHADGSVHLQADPLHKMVNPVLYRIEEAMA
                     CWKRIVAPVLWLWGDGQWMLKWMKGDKALLDSYRACYRSLHEQTIAQAGHMMHHDQPA
                     EVAREIESFLY"
     gene            1981..2427
                     /locus_tag="VN496_13640"
     CDS             1981..2427
                     /locus_tag="VN496_13640"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXF18046.1"
                     /translation="MKNTFLNRPLATILLAALTLVSLPAFAGSVEDVHEAEERRYKAM
                     IDMNPEALADSLADEFFYHQPNGKSNDKEGYIKQMMTGAVKIFSAERYDVKVNVYGDV
                     ATAMGGTRLDIELGGERRKTDLRYLNVWLKRDGRWQLAKRQSAFKQ"
     gene            complement(2488..3666)
                     /locus_tag="VN496_13645"
     CDS             complement(2488..3666)
                     /locus_tag="VN496_13645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF18047.1"
                     /translation="MKGVVWPLVVTMAIQAVVSLVVYTPPVLAPIAQADIGLPASAVG
                     IVTALIYLAATFTAFAAGGLIHRFGSLRASQMSLGMCAAGIAMMASANAWLVSLGALV
                     IGTGYGVVTPASSAILAERTPEGMRAFVFSLKQTGVPIGGALAGALIPLLIAGFGWKQ
                     AALIAGLCCLVLLIVVQPYRADVDFAAHGPRPAAPGHFLEPYRLIMAHPRLRELAFAS
                     FAYSGMQMCMGSFLVVFLTDRISLSMGAAGAALSTAMLAGSMGRIFWGVAADNWLSPR
                     TVLGSLGAVMAVSAFITASMAASWPYAAMLIVCFCYGASAIGWNGVYLSEVVRMAPPG
                     KAGSATGASLAMTYAGVVFLPTLFWLIVHVSGSYAAAFTAAGVFSLWRGSFFFKRSKH
                     "
     gene            complement(3684..4970)
                     /locus_tag="VN496_13650"
     CDS             complement(3684..4970)
                     /locus_tag="VN496_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HXF18048.1"
                     /translation="MADREFGFGTLCLHAGQIPDAATGSRAVPIYQTTSFVFDSAEHA
                     ASLFNLQTFGNVYSRISNPTVAVFEERMAALEGGRAALAAASGQAAQMCALLTILREG
                     DEIISSSTLYGGTYSQFDVTFRQFGIHTVFVNADDPENFRKAITPKTRAIYAETMGNP
                     QINVLDIEAVAQIARQAGVPLIIDNTFASPYLCQPIKFGADIVIHSATKFIGGHGTTM
                     GGVVVESGKFPWGNGKYPMMTEPSRGYHGVIFHETFGDFGYTMKCRMETMRTLGPVLS
                     PFSAWILLQGLETLHVRVERHVQNAVAVAKFLRDHPRVSWVNYPGLPDSKYHALAQKY
                     LPKGSGSIMTFGIKGGLEAGVKFIESLQFFSHLANVGDAKTLVIHPASTTHRQLSEED
                     LLKAGVGPDMIRLSIGIEDIDDILWDLDQALAQSKA"
     gene            complement(4972..5169)
                     /locus_tag="VN496_13655"
     CDS             complement(4972..5169)
                     /locus_tag="VN496_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18049.1"
                     /translation="MSKSEAKRESKNDRATTFDPFTDHRVLTPQAKTTAQHQLSITSR
                     PAFVLSTIYTIHRSLITVHRF"
     gene            complement(5198..5677)
                     /locus_tag="VN496_13660"
     CDS             complement(5198..5677)
                     /locus_tag="VN496_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXF18050.1"
                     /translation="MSDTTDINTLRRILRECKTIAVVGLSANWYRPSFFAAKYMQEHG
                     YRIIPVNPAYPEVLGEKCYASLKDIPQKVDIVDCFRKSTEIEPIADDAIAIGARVLWM
                     QLGVMNAKAADKARKAGLQVVQDRCVKIEHGRLFGGLNWVGVNTKVISSKRPRWLPY"
     gene            complement(5740..6774)
                     /locus_tag="VN496_13665"
     CDS             complement(5740..6774)
                     /locus_tag="VN496_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXF18051.1"
                     /translation="MMRLICSIVLAIMLSGCTSLGYYYQAFEGQMQIWHRSRPIEQVI
                     ADEHTPAQVKQRLALVLRIREFASAELALPDNGSYRNYADLQRPFALWNVFATTEFSV
                     TPEEWCFPFAGCVGYRGYFSQKAAEQFADSLKQEGLDVFVSGVPAYSTLGWFDDPVLN
                     TVVRYPEAELARLIFHELAHQVVYVPGDTMFNESFAAAVEQEGVERWLTRTDDKAEQA
                     AFDLQQQRRATFIELVLRYRDELQALYDSATPDTEKRQRKAAIFAAMKSEYLSVKSSW
                     DGYAGYDRFFAEPLNNARLVPVATYSELVPGFRRLLASNNGDFKRFFAAVKELGKMPE
                     EQRHAKLKET"
     gene            complement(6771..7415)
                     /locus_tag="VN496_13670"
     CDS             complement(6771..7415)
                     /locus_tag="VN496_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXF18052.1"
                     /translation="MTASNRGVVVLVHGLWMNGFAMLPLARRLERCGFQVKRHGYKSV
                     SRGLHENARGLATACENVQRSSNARLHLVGHSLGGVLIMTMLEEHPEIQVHRVVLIGT
                     PYAGSAAAHGLRQFGWSRGALGHTIEDWLRRPREPVPPGLELGVIAGTLSFGLGRLFV
                     HLPGANDGVVLLSETRVPGATESIELSISHSAMLVSPAVARATCTFLKSGSFKT"
     gene            complement(7402..8325)
                     /locus_tag="VN496_13675"
     CDS             complement(7402..8325)
                     /locus_tag="VN496_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXF18053.1"
                     /translation="MLLNQRAVIVGGGTMGADVAAIFAANGVSVDLVQRPGKTRDSLL
                     ARVTRAVRELGAAKMDVAVHDALPDVPWHQAGIVIESVVEDLSLKRKIFSELDALVPA
                     GIPLTSNSSSFPISQIGAGLKGKGRMLGLHFFMPAHLVPLVEVIRAEATELVVAQEVF
                     DLMQKLHRKPVMVAKDIPGFLANRMQHALMREAWSLIDRGIATPEDVDIAVRYGFGFR
                     YVAAGPILQKEMSGLDVNFLASSTVFPDLCNDADPAAILAAKVKAGHIGMKSGKGFYD
                     WPADKIAAEKTRYQTALKRGLEILKADDDSE"
     gene            8409..9146
                     /locus_tag="VN496_13680"
     CDS             8409..9146
                     /locus_tag="VN496_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXF18054.1"
                     /translation="MPFPLETLIAVPLIGLLAYIILGISGFGSALVSIPLLAHFLPLQ
                     SVVPLVVVVDFLATVSNGLRFREHVEVAELKLLIPAVIVGILSGVTLLATLPKHAALL
                     MLGGFITSYGAYRLVTKPAATPVSRWWGIPTGLAGGLIGGLFGVGGPIYATYMAARIH
                     DPAKMRATLSAVFSSSTGLRLALYLLSGLLLQSEVWWAFVLLLPAMPVGLVVGHRLHA
                     NLSRQQISAFVSLLLVASGLSLLWKAL"
     gene            9286..9846
                     /locus_tag="VN496_13685"
     CDS             9286..9846
                     /locus_tag="VN496_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HXF18055.1"
                     /translation="MNQQTFGSLCAHLFGLALLASTTFAPAAEPEWSNPVIKGYGKVV
                     SLPNAGMQPDKNAEYKVVFNLTAAGDPDKINASLDRVARAVNIFASAGVPPSHLHMVV
                     VIHGPATPIVLDNAHYQEKFKLDNPNIGLIDELGKVGVKVVVCGQALAGNKFAQEWVY
                     PKVEHTLSAITDLVILEKSGYVLFPM"
     gene            9878..>10244
                     /locus_tag="VN496_13690"
     CDS             9878..>10244
                     /locus_tag="VN496_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18056.1"
                     /translation="MKPQILFIALLTALFALAAKSNDSIDPPWTPSPANGKNFTIPGI
                     DNVPDLYGDINDPQLVIFFAGNQYMVVHDLLQAFQAVHPEVERIFVETLPPGVLVEQI
                     EQGALIVGNMRVSLRADIYA"
BASE COUNT         2195 a         2961 c         2918 g         2170 t
ORIGIN      
        1 tggggataca gctggatcac atccaaaatc ggtttggatt acgcaagccc gatggatgtc
       61 gtggggctgc gcctggaact cggtatcctg accttgttca tcggattgat ctggaccggc
      121 cggccgctca agccgcaaca ttggcaggcc ctgctgatga tcggcctcat acagaccggc
      181 gctttcctgt tgctcaacac gtgggctctt tccgaaggcg ggcccggcaa gacatcggta
      241 ttggtgttta ccatgccgtt ctgggtgctg gtatttgcat ggcccatgct gcaagagcgc
      301 atccaggggc tgcaatgggt agcagtcgcg ttagccatac tgggattgct gttcgtcctg
      361 gaaccctggc aaatgcacac ctcgctattc agcaaaaccc tggcggtcat agcgggaatg
      421 tgctgggcca ttggagtggt ctatgcaaag cgcctgcata accacgctaa agtcgagccg
      481 ctggcattca cgttctggca gatgatcatc ggtctgatgc cggtcattgc ctgtcagctc
      541 atgttcgagc gtccgccgat cgtgtggtcc aatacttttg tcgccgttac gatgttcaac
      601 ggcgtcatgg cgaccggcat cggctggctg gcgtggctgt atgtgttgca ccggcttccc
      661 gccggcacca ccagcctgtc gtcgctcggt atcccggtca tcgcgatcat ttcgtcctgg
      721 atacaactcg gcgagcagcc cggccgttcc gaactcgttg gcatgctgct gatcggcgct
      781 gcgctggcac tgctgtccta cctgagcatt cgccgtcatg aagagcccga gcctatgacc
      841 ggacaggagt gagacaagcc gtgaatatcg tgaatgtcgt gaatggcgtg aatgccgtaa
      901 ctgggtggtt accgccgtca cgatcgttat gggcgttagc ccggttatgt gcgttagcac
      961 ggtaccctag tggggatccc cttgcggggc acgtcgtcac gttagtcacg gaatttccgc
     1021 ggtgaaacca agctcatcca cttatctgtc catccgggga ctgcgctacc acgtgcggac
     1081 atggggcgat gcgcgcgcgc cgaagatgtt catgctgcat ggctggatgg acgtatcggc
     1141 atcgttccag ttcgtcgtcg atgaattaag acatgattgg catgtcatcg cgccggattg
     1201 gcgcggcttt ggattgtcgg aatggagtcg cgagggttac tggttccccg actacatcgc
     1261 ggacctggat ctgttgctcg agcatttcca gccggagtcc cctgcgtatg tcgtaggtca
     1321 cagcatgggc ggaaacgtcg ccaatcttta tgcagggatt cggccggaac gtatcggaaa
     1381 gctggtcctt gcggaaggct tcggactgcc gccaaccaaa cctgagaatg caccgttgcg
     1441 ctacgcgaaa tggctggaag agaaccgtga tccgcccacg ttgcggccct acaccagctt
     1501 tgaagaagtt gcagagcgcc tcaagaaaaa caattcccgc ctaagcgatg agcgcgccag
     1561 attcctggct ccgcactggg cagcccggca cgccgacggc agcgttcatc tgcaagctga
     1621 tccgctgcac aaaatggtga acccggtgct gtatcggatc gaagaagcga tggcgtgctg
     1681 gaagcgcatc gtggcaccgg tgctctggct atggggcgat ggacaatgga tgctgaaatg
     1741 gatgaaaggc gacaaggcgc tgctggacag ttaccgcgcc tgctatcggt cgctgcacga
     1801 gcagaccatc gcccaggccg ggcacatgat gcaccatgat cagccggctg aagtcgcccg
     1861 cgagatcgaa tcgtttctat actaaccatt agtccgttta tttggtggca taagacgcca
     1921 ttgccctttt gctgaagcat tacaatcgga agccgttgcc atcatgaggg aggggatgac
     1981 atgaaaaata cgtttttgaa tcgcccgctc gcgacgatct tgcttgcggc gctgacgttg
     2041 gtctcgttgc ctgccttcgc cggttcggtt gaagacgttc acgaagcaga ggaaaggcgc
     2101 tacaaggcca tgatcgacat gaatccagag gctctggccg actcgctggc cgatgagttt
     2161 ttttaccatc agccgaatgg aaagagcaac gacaaggaag gctacatcaa gcaaatgatg
     2221 acgggcgcgg tgaaaatatt ctctgcggaa cgctacgatg tgaaggtgaa tgtttatggc
     2281 gatgttgcga cggccatggg tggcacacgg ctcgacatcg aactgggcgg agaacgccgc
     2341 aaaacggact tgcgctattt gaatgtgtgg ctcaagcgcg acggccggtg gcaactcgcc
     2401 aagcggcaat ctgcattcaa gcaataagcg ttgtctggcg caatcctcga cggccatggc
     2461 ctcgcacgga tagagcaaca ccgggtatca gtgcttgctg cgcttaaaga agaacgagcc
     2521 gcgccaaagc gaaaacacgc ccgcggcggt aaacgccgcg gcgtaactgc cactgacgtg
     2581 cacgatgagc cagaacagcg tcggcagaaa caccacgcca gcgtaggtca tcgccaggga
     2641 agcacccgtc gctgatcccg ctttgcccgg cggcgccatg cgaaccactt cggaaagata
     2701 aacgccgttc cagccgattg cgcttgcgcc atagcagaag cacacgatca gcatggcggc
     2761 gtaaggccag gacgcggcca tcgaggcagt tatgaacgcc gacaccgcca tgactgcgcc
     2821 aagcgagccg agcaccgtgc gcggcgataa ccaattatcc gcggcaacgc cccagaagat
     2881 gcgccccatg ctgcccgcca gcattgccgt agacaacgcc gctccagcag ctcccatcga
     2941 caagctgatg cgatccgtca gaaataccac cagaaacgat cccatgcaca tttgcatgcc
     3001 cgaataagca aacgacgcga aggccagttc acgcagtcgc gggtgcgcca tgatgagccg
     3061 gtacggttcc aggaagtgcc ctggcgcagc aggcctgggc ccatgagccg cgaaatccac
     3121 atccgcgcgg tacggctgca cgacaatcaa caggacaaga caacacagac cggcaatcaa
     3181 agcggcctgc ttccaaccga atccagcgat cagcagtgga atcaaggcac ccgccaaagc
     3241 acccccgatt gggacgcccg tctgcttcaa cgaaaaaaca aaagctcgca tgccctccgg
     3301 cgtgcgttcg gccaggatgg ctgaacttgc cggcgtgacc acgccgtatc ctgtgccaat
     3361 cacgagtgcg ccaagtgaaa ccaaccaggc atttgcactt gccatcattg cgattcccgc
     3421 agcgcacatg ccaagcgaca tctggctggc ccgcaaagaa ccgaatcggt gaatgaggcc
     3481 gcctgccgcg aacgcagtaa aagtcgcggc cagatagatc agcgctgtaa caatcccgac
     3541 cgcgctggcc ggcaatccaa tatcagcctg tgcaatgggt gccaggactg gtggcgtgta
     3601 aaccaccagt gaaaccacag cctggatcgc catggtgaca accaatggcc agacgacgcc
     3661 tttcatccaa aacggttaag ggtctaagct ttggactgcg cgagcgcctg atcgagatcc
     3721 cacaggatat cgtcgatatc ttcgatgccg atcgacagac ggatcatgtc cgggccgacg
     3781 ccggccttga gcaggtcttc ttcggacagc tggcggtgcg tcgtcgaagc gggatgaatg
     3841 accaatgtct tggcatcgcc gacattagcc agatgcgaaa aaaactgcag gctttcgatg
     3901 aacttcaccc cagcctccag tccgcctttg atgccgaaag tcatgatcga accgctgccc
     3961 ttcggcaggt atttttgggc cagcgcatga tatttgctgt ccggcaggcc cgggtaattg
     4021 acccacgaca cgcgcggatg gtcgcgcagg aacttcgcca ccgccacggc attttgcaca
     4081 tgacgctcga cgcgcacgtg cagcgtttcc aatccctgca acagaatcca ggcgctgaaa
     4141 ggcgaaagaa ccggtccaag agtgcgcatg gtttccatgc ggcatttcat ggtgtagccg
     4201 aaatcaccga atgtctcgtg aaaaatgacg ccgtggtagc cgcgcgacgg ttcggtcatc
     4261 atcggatatt tgccgtttcc ccatggaaac tttccggact cgaccacgac cccgcccatg
     4321 gtcgtgccat gcccaccgat gaacttggtg gcggaatgaa tgacgatgtc ggcgccaaac
     4381 ttgatcggct ggcacaggta cggcgacgcg aacgtattgt cgatgattaa cgggacgccc
     4441 gcctgcctgg caatctgtgc cacggcttcg atatcgagca cattaatctg cggattgccc
     4501 atggtctctg cgtaaattgc ccgcgtcttc ggtgtaatcg ccttgcggaa attttcagga
     4561 tcgtccgcat tcacaaaaac ggtatggatg ccgaattggc gaaacgttac gtcgaactgt
     4621 gaatacgtgc cgccgtacag cgtgctggaa gaaatgattt cgtcgccttc gcgcaggatg
     4681 gtcagcagcg cgcacatctg cgcggcctgc ccggaagcgg cggcgagcgc ggcccgcccg
     4741 ccttccagcg ccgccatgcg ttcctcgaac accgccaccg ttggattgga tatgcgtgaa
     4801 taaacgttgc cgaacgtttg cagattaaac aggctggccg catgttcggc ggaatcgaac
     4861 acgaacgatg tggtctggta gatcggcact gcgcgtgagc cagtggccgc gtccggaatt
     4921 tgccccgcgt gcaggcacaa cgtgccgaac ccgaattctc tatctgccat attaaaaccg
     4981 gtgaacggtg atgagtgaac ggtgaatagt gtaaatagtg ctcagaacaa atgccgggcg
     5041 gctcgtaatg gacaattgat gctgcgcagt agtttttgct tgcggcgtaa gtacacggtg
     5101 atcagtaaac gggtcaaaag ttgtggcgcg gtcgtttttg ctttcgcgtt ttgcttcgct
     5161 tttgctcatc accgttcacc attcaccgtt cactgcatca atacggcagc cacctcggcc
     5221 gtttggagga aatgactttt gtgttcacgc ccacccagtt gaggccgccg aatagacggc
     5281 catgctcgat tttcacgcag cggtcctgca cgacctggag gccggccttg cgcgctttgt
     5341 cagccgcctt tgcattcata acgcccagtt gcatccacag gacccgcgcg ccgattgcta
     5401 tcgcatcgtc agcgatcggc tcgatttcgg tactcttccg aaagcagtcg actatgtcca
     5461 ctttctgcgg aatgtctttc agggacgcat agcacttttc gcccaggact tccggatatg
     5521 ccggattgac cgggataatg cggtagccgt gttcctgcat gtactttgca gcgaagaagc
     5581 tgggccggta ccagttggcc gaaagtccaa ccacggcaat ggtcttgcac tccctgagaa
     5641 tgcgacgcaa ggtattgatg tcggtggtgt cgctcatacg gaaagtccgg aaatgtggct
     5701 gtgcgaaaga gcggcagttt atcaccagcg ctaccgtctt caggtctcct tcagtttcgc
     5761 atgccgctgt tcttcaggca ttttgcccag ctccttcacc gccgcaaaaa aacgcttgaa
     5821 atctccgtta ttgctggcca ggaggcggcg gaagccgggt accagctcgg agtatgtcgc
     5881 caccggcaca agccgggcgt tgttgagtgg ctcggcaaaa aagcgatcgt agccagcata
     5941 accatcccat gaagatttca cgctcaggta ctcggacttc atcgccgcga aaatcgcagc
     6001 ctttcgttgt cttttttcgg tatccggggt tgcactgtcg tatagcgcct gaagttcatc
     6061 ccggtatctc agcaccaact cgataaaagt ggcacggcgc tgttgttgca agtcaaaagc
     6121 ggcttgctca gctttgtcat ccgttcgcgt cagccatcgt tcaacgcctt cctgttcgac
     6181 agccgcagca aaagactcgt tgaacatggt atcgcccgga acatagacca cctggtgcgc
     6241 cagttcgtgg aaaatcagcc gggccagttc agcttccgga tagcggacga cagtattgag
     6301 cacgggatcg tcgaaccaac ccagcgtgga gtaggccgga acccctgaca caaaaacatc
     6361 cagaccttct tgcttcaggc tgtcggcgaa ttgttcggcg gctttctggc tgaaataccc
     6421 gcgatagcca acgcatcccg caaacggaaa gcaccactcc tcaggcgtga ccgagaactc
     6481 tgtcgtcgcg aaaacattcc agagggcaaa cggccgctgc aggtcggcgt agtttcgata
     6541 gctgccgttg tcgggcagcg ccaactctgc ggacgcaaat tcccggatgc gcagcaccag
     6601 cgcgaggcgt tgcttcactt gcgcaggcgt atgttcatcg gcgatgactt gttcgatggg
     6661 gcggctgcga tgccagatct gcatttgccc ttcgaaggcc tgatagtaat agccgaggct
     6721 ggtgcaaccg gaaagcatga tggcaagcac gatcgagcaa ataagccgca tcatgttttg
     6781 aaacttccgc ttttcaaaaa tgtacacgtg gcgcgcgcaa ctgcgggcga caccagcatg
     6841 gcgctatggc tgatactcag ttcgatggac tccgtcgctc cgggtacccg tgtttcactg
     6901 agcaacacca ccccgtcatt cgctcctggc agatgcacaa acaatcggcc gagaccgaat
     6961 gaaagagtgc ccgcgatcac gcccaattcg aggcccggtg gaacaggctc gcgcggccgg
     7021 cgcaaccagt cttcgatggt atggcccaat gcgccccggc tccagccaaa ctgtcggagg
     7081 ccgtgcgctg cggcgcttcc cgcataggga gtgccgatca gcacgacccg atgcacctgg
     7141 atttccggat gctcctccag catggtcatg atcaatacgc cgccaagact gtggcccacc
     7201 agatgcaatc gtgcgttgct gcttctttgc acgttttcgc acgccgttgc tagcccacgc
     7261 gcattctcat gcaagccacg agaaacagac ttatagccgt ggcgttttac ctggaaaccg
     7321 caacgttcga ggcggcgcgc caaaggcagc atggcaaaac cgttcatcca caagccatga
     7381 acaagcacca ccacgccacg gttactcgct gtcatcgtcg gctttaagaa tctccaagcc
     7441 acgcttcaac gcggtctggt agcgggtttt ctcggctgca attttgtcgg ccggccagtc
     7501 gtaaaaacct ttgcccgatt tcatcccgat gtggccggct ttcactttcg cggccagaat
     7561 cgccgccgga tcggcgtcgt tgcaaagatc cggaaacacc gtgctcgagg ccagaaaatt
     7621 cacatcgagc ccggacattt ctttttgcag gattggccct gcagcaacat aacggaatcc
     7681 gaagccatat cgcaccgcga tatcgacgtc ttcgggcgtt gcaataccgc ggtcgatcag
     7741 cgaccacgct tcgcgcatga gggcatgctg catgcgattg gccagaaatc cgggaatatc
     7801 cttggcgacc atcaccggtt tgcggtgcag cttctgcatc aaatcgaata cttcttgtgc
     7861 caccacgagc tccgtagcct cggctcgaat cacctcgacc agcggcacca gatgtgcggg
     7921 catgaagaag tgcagtccca gcatgcgacc cttgccttta agtcccgctc cgatctggct
     7981 aatcggaaag ctggacgaat tgctcgtgag cggaataccg gcagggacaa gcgcgtcaag
     8041 ttcgctgaag atcttgcgtt tgagcgacaa atcttcgaca accgattcta tgacgatgcc
     8101 ggcctggtgc catggcacgt cgggcaacgc gtcgtgcacc gcaacgtcca ttttcgcggc
     8161 gccgagttca cgtactgcgc gggttacccg cgctagcagg gaatcacgcg tcttacccgg
     8221 gcgctgcacg aggtccacgc tcacaccatt cgccgcgaaa atcgcggcga cgtcagcgcc
     8281 catggtccca cccccaacga tcacggctcg ctgattgaga agcacggcgt gtctcctgtt
     8341 catcgattcg aggcggcatt ataaatgcgc gggagtgcta gacttcgcgc cttcttttgc
     8401 cgaccaacat gccttttccg cttgaaacgc tgattgccgt tccgctgatc ggactgctgg
     8461 cctatatcat cctcggcatt tcgggctttg gttcggcgct cgtttccatt ccgctgctgg
     8521 cgcatttcct gccactgcaa tcggtggtgc cattggtcgt ggtggtcgat tttctggcta
     8581 ccgtgtccaa cggcctgcgt tttcgggaac atgtcgaagt ggctgaactg aaattgctga
     8641 ttcctgccgt aatcgtcggc atcctgtcgg gagtgacgct gcttgcgaca ctgccaaagc
     8701 atgccgcgct gctgatgctg ggcggattta ttacgagcta cggcgcatac cggcttgtca
     8761 ccaaacccgc cgctacaccg gtttcgcgct ggtggggtat cccgaccggc ctggcgggtg
     8821 ggctgattgg tggattgttc ggggtcggtg gtccgattta cgctacgtac atggcagcgc
     8881 gcattcatga tccggcaaaa atgcgtgcga cgttgtccgc cgttttttcc tccagcaccg
     8941 ggctgcggct ggcgttgtat ctgctctccg ggttgttgct tcaatcagag gtctggtggg
     9001 cctttgtgct tctgctgccc gcaatgcccg ttggactcgt cgtcggccac cgcctgcatg
     9061 cgaatctttc ccgccagcag atcagcgcat tcgtcagcct gctgctggta gcgagcggtt
     9121 tgtcattact gtggaaggcg ctatgaaccg tgctctgaaa tacagcaagg tcaactgtat
     9181 cggttaaggt gcgttggtaa tcggcaggca gaggcgctgt ctcgcatcgc tatcattgcc
     9241 atttagaagt ggacagtcgt tatttgatat caaggagaac tcaatatgaa tcagcaaact
     9301 tttggatcac tgtgcgcgca tctgttcgga cttgctctgc tggcctccac aacgtttgcg
     9361 ccggctgcgg agccggaatg gagcaatccg gtcatcaaag gttacggcaa ggtcgtgtcc
     9421 ctgcctaacg ccggaatgca accggacaag aatgcggaat acaaggtcgt gttcaacctg
     9481 accgccgccg gcgatccgga caaaatcaac gcatcgctcg atcgggtcgc acgcgcggta
     9541 aacatctttg cttcggccgg cgtgccgccc tcgcatctgc atatggtcgt cgtcattcat
     9601 ggacccgcta ctccaatcgt gctggacaat gcgcactacc aggagaaatt caagctggac
     9661 aacccgaaca tcgggctgat cgatgaattg gggaaagtgg gcgtaaaagt agtcgtatgc
     9721 gggcaggccc tggcaggcaa caagttcgcg caggaatggg tttatccgaa agtcgagcac
     9781 acgctatcgg cgatcaccga tctcgtgatt cttgaaaaga gcggatacgt attgtttccg
     9841 atgtgaacaa gtgcgcggta aatatgcggc aaatataatg aagccgcaga tactgttcat
     9901 tgcgttgctg acagcgttgt ttgctctcgc ggcgaagagc aatgacagca tcgatccgcc
     9961 gtggacgccg tcaccggcaa acgggaagaa ttttacgatc ccgggcatcg acaacgtccc
    10021 ggatctttac ggcgacatca atgatccgca gctggttatt ttcttcgcgg gcaatcaata
    10081 catggtggtg cacgacctgc tgcaggcatt ccaggcagtg catcctgaag tcgaacgcat
    10141 ttttgtggag accttgccgc ccggcgtcct ggtcgagcag attgaacaag gcgcgttaat
    10201 cgtcggcaac atgcgggttt cactacgcgc ggacatctac gctg
//
LOCUS       DATMZQ010000429         6244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4662690, whole genome shotgun sequence.
ACCESSION   DATMZQ010000429 DATMZQ010000000
VERSION     DATMZQ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4662690"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VN496_13695"
     CDS             <1..203
                     /locus_tag="VN496_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18057.1"
                     /translation="SKQQAKDNPAVHLFCARYQMAYLLIQLLVYGDTTVGFLRQLSYL
                     RRPYVVCELQVEWNNRGDVDRV"
     gene            complement(352..1833)
                     /gene="zwf"
                     /locus_tag="VN496_13700"
     CDS             complement(352..1833)
                     /gene="zwf"
                     /locus_tag="VN496_13700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXF18058.1"
                     /translation="MSEEDALYPCSFVIFGATGNLAATKLLPALYQLELAKRLPDVTN
                     FVVIGRRPWKESDWASHMEQALRKKVGKQFTPECFGRFAARFSYIRGDLQEPEAYKRL
                     LDELGKPKTGVCSNIVFYLAVKPEDFGVIVENLARVGLNRPRGLHRIVVEKPFGEDIS
                     SAQQLNQLLHRYFDEQQVYRIDHYLAKESVQNLLVFRFANLMIEPLWNRNFVDHVQIT
                     VAEQVGIETRADYYDRIGALRDMLQNHLMQLLTLVAMEPPAALEADTLRDEKVKVLRS
                     IRPIAADAVQNHAVRGQYGPGVLDGRQVPGYTAEAGVPPGSATPTYVAAKFFIDNWRW
                     RGVPFYLRTGKRLATQLSVIALRFRHPPQQLFRETPLENMTPNWIILSLQPNESMRLE
                     IHAKQPGLGMHTRVIQMNAGYRQDHEPQPDAYEALLADIIRGDATNFIRFDEVEWAWR
                     VVDPILKHWIEHDNSTYPYSAGSWGPQEADRLLASGQAWRDHI"
     gene            2015..2656
                     /locus_tag="VN496_13705"
     CDS             2015..2656
                     /locus_tag="VN496_13705"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HXF18059.1"
                     /translation="MNYLAHLYLSAPTEDAWLGSLLGDFVKGPLDQRYSQHITDAIAL
                     HRRIDSFTDAHPVVLRSKSRISAVRRRYAGIMIDMFYDHFLASNWSEFHDEPIKVFTA
                     RIYEILRVRRAILPDRLNTMAPRMAQSDWLASYADIDTIHSALDRMGRRFKRENRLLD
                     SADELREHYAELESDFRIFLPQVVQFSQQIYMPDRLSSDTPPEPGPDTRDGGE"
     gene            complement(2601..2903)
                     /locus_tag="VN496_13710"
     CDS             complement(2601..2903)
                     /locus_tag="VN496_13710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF18060.1"
                     /translation="MPAQTSFAVIHNVAAKRFEVNVDGKIAFSKYLLVAEKMIIEHTE
                     VPSELEGRGIAGTIVQTALEYARAQRLKVMPLCPFAASFIHRHPEYQDLVLEGYRY"
     gene            complement(2927..4330)
                     /locus_tag="VN496_13715"
     CDS             complement(2927..4330)
                     /locus_tag="VN496_13715"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXF18061.1"
                     /translation="MREELSFIPASELVRMIKQKIVSPVEVMRQTIARAEALNPRLNA
                     ICTPTFEAAMEAAAEAEIAVMRGHPLGILHGVPTSIKDLAFTRGVRTMAGSHIYRDRI
                     PDFDHAHVERLRKAGAISIGKTTVSEFGWSGVSNSPLTGITHNPWQHGMNAGASSAGA
                     AVCAAAGIGPIHQGSDGAGSIRMPAAFCGVYGIKPSHGRVPYWPTPANGLISHVGPLT
                     RTVTDAALMLQAMAGPDDRDMTSLESEPEDFAGRLDEGIGQLRIGYSPDLGYLKVDAE
                     VSTLVGTAVAAFEELGCHVDEIQPGWDNPIEMEHLHFSANFASRLEPLLEKWHDQMDP
                     GLVALVKHGMQFSAAEYCRLDEKRLALYDKVRSLFERYDLLLTPTLSVAAFPAERLMP
                     QHWEQHPWDWMRWAGFSYPFNLTWLPAASCPCGFTKSGLPVGLQIVAGRHRDLRVLQA
                     SRAFERIRPWAQHRPPC"
     gene            complement(4371..5426)
                     /locus_tag="VN496_13720"
     CDS             complement(4371..5426)
                     /locus_tag="VN496_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HXF18062.1"
                     /translation="MLSYDVVEWGKPLQKAEKQTPQPTGTEVLLKLKYCGVCHSDVHI
                     RDGYFNLGGGKRFYMSERGMHPPVTLGHEPFGTVIAAGVDAKNVPIGQDRLVCPWIGC
                     GKCARCLEGMDNHCMAPRWIGIQRPGGYADHLLVPHAKYLVDASGIDAMWAATLSCSG
                     LTTYSAVAQLKPIPGEEWVAVFGAGGLGLMAIEMLKALGHSRIVVVDIDDDKLAVARK
                     EGAAATVNGRDPEAAKKLLEAAGGPLYGAVDLVGAADTATLALGALRKGGKLIVVGLY
                     GGEIPVSLVLVVQRAWRIQGSSVGNVAELNEVIALARAGKIKPIPIEKRPLSEVSRTL
                     DQLKAGTVVGRVVAAIS"
     gene            complement(5413..5943)
                     /locus_tag="VN496_13725"
     CDS             complement(5413..5943)
                     /locus_tag="VN496_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXF18063.1"
                     /translation="MISDDIRAITSYHNKVRADVGVGPLQWSGDLAGYAQQWASHLAA
                     TTCSMAHRTQQKYGENLFQGTAGYYPAVDADKAWENEKKKLLRRHPDRSELATGGALH
                     ANGMARHDDFGLRPVDMRPNCHRGLQLCTARQLPWSSALLSPVTPAFALRHTARCRIQ
                     PHRVQAKTKGERNAEL"
BASE COUNT         1283 a         1851 c         1742 g         1368 t
ORIGIN      
        1 agagcaagca acaagcaaaa gacaatccag cagtgcattt gttctgcgcc aggtatcaaa
       61 tggcatattt actgatccag ttgttggtgt atggcgacac aaccgttgga tttttgcgtc
      121 aactcagcta tctcagacga ccgtatgtcg tctgtgagct acaggtcgaa tggaataatc
      181 gcggagatgt agatcgcgtg taactgctgg agatcgcctc atggaaacgt gcacagcgac
      241 atttgattgc gcctgcagat caggcgaagt ttaattgggc tgtgcgacgc tttagtgatc
      301 cagtactttt gttatggcgc agtggtgcgt tcgacaaaaa tttgtgagcg ctcagatatg
      361 atcgcgccat gcttggccgc tggccaacag ccggtcggcc tcctgtggtc cccaactacc
      421 cgcggagtac gggtaagtgg agttgtcatg ctcgatccaa tgcttgagga tcgggtccac
      481 cacccgccat gcccactcca cctcgtcgaa gcggatgaag ttggtagcat cgccacggat
      541 gatatcggcg agcagcgcct catatgcatc aggctgcggc tcgtgatctt gtctgtagcc
      601 tgcgttcatc tggattacgc gcgtgtgcat tccgagcccc ggctgtttgg catggatttc
      661 cagacgcatg ctttcgttgg gctgcagact cagaataatc cagttgggtg tcatgttttc
      721 caatggtgtt tcgcgaaaca actgttgcgg cggatggcga aatctcagtg cgatgacaga
      781 taattgagtc gccagccgct tgccggttcg taaataaaaa gggaccccgc gccagcgcca
      841 attgtcgatg aagaatttgg cagcgacata ggtcggggtc gcagatccgg gcggcacgcc
      901 ggcttcagcg gtataaccgg gcacttgtcg accatccaga acgcctgggc cgtattggcc
      961 ccgcacggca tggttctgta cggcgtcggc ggcgatcgga cgaatcgaac gcaacacctt
     1021 cactttttca tcgcgcagcg tgtccgcttc caacgctgcc ggcggctcca tggcaaccag
     1081 cgtaagcaac tgcataaggt ggttttgcag catatcgcgc aacgcgccga tcctgtcata
     1141 gtagtcagca cgcgtttcaa taccgacctg ctccgcaact gtgatctgga cgtgatcgac
     1201 gaaattacga ttccacagtg gttcaatcat caggttggca aaacggaaaa ccagcagatt
     1261 ctgcacgctc tccttggcga ggtagtgatc aatgcgatag acctgttgct catcgaagta
     1321 acgatgcaat aactgattaa gctgctgcgc actgctgatg tcttcgccga agggtttttc
     1381 caccacgatt cgatgcagcc ctctcggccg attcaggccg accctcgcca ggttctctac
     1441 gataacgccg aagtcctcgg gctttacggc aagataaaag acaatgttgg agcagacgcc
     1501 cgtttttggc ttgcccagct catcgagaag tcgtttgtaa gcttccggct cctgcaaatc
     1561 gccgcgaatg taactgaagc gagcggcaaa ccgcccaaag cattccggcg taaattgctt
     1621 gccgactttt tttcgcaacg cctgctccat atgggatgcc caatcggact ccttccacgg
     1681 acgccgccct attacgacaa agttagtgac atccgggagc cgcttggcga gttccagttg
     1741 gtacaacgcc gggagcaatt tcgttgcggc aagattcccc gtggccccga agatgacaaa
     1801 actacagggg tataaggcgt cttcctcact catatttttg cctgatgggt aactagacaa
     1861 agcatttgtt tttacccgta gccgaagctg ataatgcggt tcgacgcgca aaccttttcg
     1921 gcattcctat aatgccaatg gacgaccaca ccgttcggag gtcctttatc tcgaactcag
     1981 gcggacggga ctcgtcaacc agcacaccgt cactatgaat tacctggcac atctctacct
     2041 ttcggcgccc acggaagatg cctggctcgg cagcctgctg ggcgatttcg tgaaggggcc
     2101 gctggaccaa cgttacagtc agcacatcac cgacgccatc gcgctacacc gaagaataga
     2161 cagctttacc gacgcacatc ccgtggtctt gcgcagcaag tcgcggatca gcgccgtccg
     2221 gcgccgctac gccggcatca tgatcgacat gttctacgat catttcctgg cgagcaactg
     2281 gagcgagttt cacgacgaac cgatcaaggt atttaccgcc cggatatatg agattcttcg
     2341 cgtgcggcgc gcaatactgc cggatcgact gaacaccatg gcaccacgga tggcgcagtc
     2401 ggattggctc gcgtcctatg ccgacatcga caccatccac agtgcgcttg atcgcatggg
     2461 ccgccggttc aagcgagaaa accggttgct cgactccgcc gacgaacttc gggaacatta
     2521 cgctgaactc gaatccgatt tccggatatt cctgccgcag gtcgttcagt tctcacaaca
     2581 gatttacatg cccgacaggc tcagtagcga tacccctcca gaaccaggtc ctgatactcg
     2641 ggatggcggt gaatgaagct ggcggcgaac gggcagagcg gcattacttt caggcgttgc
     2701 gcgcgcgcgt attcaagcgc ggtctgcaca atcgtgcccg cgatgcctcg gccttccagt
     2761 tcactgggga cctcggtgtg ttcgatgatc atcttttctg cgaccagcaa atatttcgag
     2821 aacgcgattt tgccatccac attgacttcg aagcgcttgg cggcgacgtt atggatcacg
     2881 gcgaaactgg tttgtgccgg catggctgtt tagtccaggt agccgatcaa cagggcggac
     2941 gatgctgcgc ccagggtcga atgcgctcga acgcacggga ggcttgcagc acgcgcagat
     3001 cccgatggcg gccggccacg atttgcaaac caaccggcaa gccggatttg gtaaacccgc
     3061 aaggacagct cgcagcggga agccaggtga gattgaaggg ataactgaag cccgcccaac
     3121 gcatccagtc ccacggatgc tgctcccagt gctgcggcat gagacgctcg gcgggaaatg
     3181 cagccactga cagtgtgggc gtgagcaaca gatcataacg ctcgaacagt gaccggacct
     3241 tgtcgtacag cgcaaggcgt ttttcgtcga gacggcagta ttcggcggca ctgaactgca
     3301 tcccgtgctt gaccaatgcc acgagtccgg gatccatttg atcgtgccat ttctcgagca
     3361 atggctccag gcggcttgcg aagttcgcag aaaaatgcag gtgttccatt tcgattggat
     3421 tgtcccaccc cggctgaatt tcatcgacgt ggcagcccag ttcctcgaaa gcggccaccg
     3481 cagtgccgac cagtgtggat acttccgcat ccactttgag gtaacccaga tccgggctgt
     3541 acccaattct gagttgcccg attccttcat cgagacgtcc ggcaaaatcc tccggctctg
     3601 actccaggct ggtcatgtcc cgatcgtccg gtcctgccat tgcctgcaac atcagcgccg
     3661 catcagtgac ggtgcgggtt agcggtccaa catgcgagat cagcccattg gcgggcgtgg
     3721 gccagtacgg tacacggccg tgcgacggct tgatgccata tacgccgcaa aatgcggctg
     3781 gcattcgaat cgatcctgcg ccatccgaac cttgatggat tggcccgatg cccgctgccg
     3841 cacacactgc cgcacctgcg ctggaagcgc cggcattcat accatgctgc cagggattgt
     3901 gcgttatgcc agtcagcggg ctattgctca cgccgctcca gccaaattcc gaaacggtgg
     3961 ttttgccaat ggagattgca ccggccttgc gcagacgctc aacatgcgca tgatcgaaat
     4021 ccggaatgcg atcgcggtag atatgcgagc ccgccatggt gcgcacccca cgcgtaaagg
     4081 ccaaatcctt gatgctcgtc ggtacaccgt gaagaatgcc taaagggtgg ccgcgcatga
     4141 ccgcaatttc agcttcggct gccgcctcca tcgccgcctc gaatgtcggt gtgcagatcg
     4201 cgttaagccg cggatttagc gcttctgccc gcgcaatggt ctgccgcatg acttccaccg
     4261 gagacacgat cttctgtttg atcatgcgaa ccagttcgct cgccggaatg aatgacagtt
     4321 cttctctcat tgccagggct ccatcaactg cgctttagcc ggcgatacgc tcaactgatc
     4381 gcagcgacaa cccgtccgac cacggtcccc gctttgagtt gatccagcgt gcggctcact
     4441 tccgacagcg ggcgcttttc gattggaatc ggtttgatct tgcctgctcg tgccagcgcg
     4501 atgacttcat ttaactctgc aacgttgccg acgctggacc cctggattcg ccaggcacgc
     4561 tgcacgacaa gaaccagcga gaccggaatt tcaccgccat agagtccgac cacgatcagt
     4621 ttcccgcctt tgcgcaatgc gcccaatgcg agggtcgccg tgtctgctgc accgaccaga
     4681 tcgactgcgc cataaagcgg cccgccagcg gcctcgagca gcttcttcgc ggcttcagga
     4741 tcacgtccat tcaccgtcgc ggccgccccc tccttgcgcg caacggccag cttgtcgtcg
     4801 tcgatatcga cgacgacgat acgcgagtgg ccgagggcct tcagcatttc gatcgccatc
     4861 aggccgaggc cgccggcccc aaatacggct acccattctt cacctggaat gggctttagc
     4921 tgcgcaacgg cggaataagt agtcagtccc gagcaggaca aggtggccgc ccacatggca
     4981 tcgatgccgc tcgcatcgac cagatacttc gcatggggca ccagcagatg gtcggcgtag
     5041 ccgcccggcc gctggatgcc aatccagcgc ggggccatgc aatggttgtc catgccctcg
     5101 agacaacgcg cgcacttgcc gcagccaatc caggggcaca ccaggcgatc ttgtccaatg
     5161 ggtacgtttt tggcatcgac acccgcggcg atgacggtgc caaagggttc atggccaagc
     5221 gtaaccggcg gatgcattcc gcgctcactc atgtagaagc gtttgcctcc tccgagattg
     5281 aaataaccat cgcggatatg cacgtcgctg tggcagaccc cgcaatactt cagcttcaac
     5341 agcacttcgg taccggtcgg ctgcggcgtt tgcttctcgg ctttctgcag cggtttgccc
     5401 cactccacga cgtcataact cagcattgcg ttcccctttt gtttttgctt gcactctatg
     5461 cggctgaatc ctacaccgag cagtgtgcct cagtgcaaac gccggtgtta cggggcttag
     5521 tagggccgac gaccaaggta gttgcctggc ggtgcatagt tgcaaaccac gatgacagtt
     5581 tggccgcatg tcgactggcc gcaacccaaa gtcgtcgtgt cgcgccatac catttgcgtg
     5641 tagtgccccg ccggttgcca gttcgcttct gtcaggatgc cgccggagta attttttttt
     5701 ctcgttttcc cacgccttgt ccgcatcgac cgcagggtaa tagccggctg taccttgaaa
     5761 tagattttcg ccatattttt gctgggtgcg gtgcgccatg ctgcaggtcg ttgcggcaag
     5821 gtggcttgcc cattgttgcg catagcccgc gagatcgccg gaccactgca gtggcccaac
     5881 cccgacatcg gcgcgcactt tgttgtgata cgaagtgatg gcccggatat cgtcggagat
     5941 catattgctg cctgggttgc ttttcgctga atcagccggc tgggctacgg ggacagcggc
     6001 agatttcggc gggtctccgg tgcagcttgc ggttagcagg aatgctaatg caagtccggt
     6061 gtgatcgggc ctcttcatgc ggtcgtgctc atcgggtgcg atacttgtcc atgaccgcgt
     6121 tgaggcgagc gttcgatggg gcttgctgcg tatagccgaa ggttccgctt tcgcgcagtt
     6181 cacccatcga gtgccgcacg aagccgagca ccgcggccga caagctcccg cctatgctca
     6241 cacg
//
LOCUS       DATMZQ010000430         8968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4662705, whole genome shotgun sequence.
ACCESSION   DATMZQ010000430 DATMZQ010000000
VERSION     DATMZQ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4662705"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VN496_13730"
     CDS             <1..1007
                     /locus_tag="VN496_13730"
                     /inference="COORDINATES: protein motif:HMM:NF044767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HXF18064.1"
                     /translation="LFSPRLTGQLYRLTRGVPRVINVLCDRALLGAYVQGKERVDAAT
                     LAQAAREVFHQTDDRRNVLRALIATLILTAGVAVAFTLHRQDMNDARMSIPVRGSVKP
                     AASPSPATAEPTGASVVAAAATSTSALADSLEWRAGEGLASNRAQAFAVLLKAWGQAF
                     DQSADPCKQAEGAGLRCHSARGGLDELRELNRPAVLRMRNAAGEEFAATLMALGEKTA
                     SFALGDQSATISLDTLASQWSGHYTLLWRMPPPQVRDSIRPGERGAAVQWLAGQLALV
                     RGLSAPASTDPVFNEELQREVKKFQLVHGLIPDGSVGPQTLMHLASWGDPTAPKLSSK
                     "
     gene            1023..1799
                     /locus_tag="VN496_13735"
     CDS             1023..1799
                     /locus_tag="VN496_13735"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HXF18065.1"
                     /translation="MSFILDALRKSDQQRQRGSAPTLLTAQAAAIGTKRPSLMAYGAL
                     GLVLLAAGIVIGWLRPWQEQVAPVPPSPPKPISTPSRPEPETAQRPSAAPSAPAPQAA
                     VPDKSPSPGADAIPKSSGQQEAGTPASAQTVKTVPEQRTKQATEKPRAPLSQAATISS
                     RPPQPAQVPAVNADGSSGASEQPPPWMTELPVEVQRDLPAMHVMVHAYSARPTDRLVG
                     INNKLLHEGDEVAPGLKLEQITPEGMILNFKGQVFRRGVH"
     gene            complement(1867..2292)
                     /locus_tag="VN496_13740"
     CDS             complement(1867..2292)
                     /locus_tag="VN496_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18066.1"
                     /translation="MIRARCHHFGAMILLGLTSTALACGYCIEDKIAVVYDYGVVTHA
                     LSRHHQVVFFAVEGTIVPNEKLRRALKTAVESLSGVDKGSTRVSGAPAAVSAAFDPTR
                     VSAAAMEQALSRKLASHGLSITTLRVMAGQSALTPAGKP"
     gene            complement(2289..3719)
                     /locus_tag="VN496_13745"
     CDS             complement(2289..3719)
                     /locus_tag="VN496_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXF18067.1"
                     /translation="MACTALVLGLAACNQSAPPDRYDQAKMDLKPLPSKMSSERHTGA
                     FSSGVALSTAPDIKPVEASPVKTIQLDTTHKIIEIAPGIRFSGWTFGDQVPGPVIRAR
                     VGDKIKFSMTNRSDEVAPGLQMMIPPMMHSMDFHAAMVSPQDKYHSIAPGQTITFEFT
                     LNYPGVFMYHCGTPMILEHIASGMYGAIVVEPREGYPTKVDREYVVIQSEFYPKLDPQ
                     KRSIDGAPLYVLDGERLRAASPGYTVFNGVHNGMVKKPLAAKPGERVRLFVLNVGPSK
                     TSSFHVVGTIFDRVWLDGNPDNQLRGMQTVLLGSSSGAIVEFVIPEAGQYIMVDHHFA
                     NASQGAIGLIDAGGPPEEKGEIEHHNIPATRTPGDPEAVKGKLDFESKCLACHSIGQG
                     RKLGPDLAGVTKRRSNEWLTKWLKTPEKMLEVDADAKAMLKEAGGLPMPNQNLSDTEV
                     QQYIKYFKWADEQNATGGHDAKEGKR"
     gene            complement(3937..4590)
                     /locus_tag="VN496_13750"
     CDS             complement(3937..4590)
                     /locus_tag="VN496_13750"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXF18068.1"
                     /translation="MLLFSKTAGFRHDSIPAGIDAIHMLGERNGFRVDASEDAGAFTD
                     ANLARYRAVIFLNTTGEILEADQRAAFERFIQHGGGFVGIHAASDTGYQWPWYGGLVG
                     TYFQRHPAIQPATLKVVDASHASTRHLPITWKRTDEWYDFRHALDPDITVLIRIEEAT
                     YRGGRMGADHPIAWHHQYDGGRGWYTAIGHTVESYREPLVLEHLSGGILWAAGALPE"
     gene            complement(4695..4877)
                     /locus_tag="VN496_13755"
     CDS             complement(4695..4877)
                     /locus_tag="VN496_13755"
                     /inference="COORDINATES: protein motif:HMM:NF026900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm17 family immunity protein"
                     /protein_id="HXF18069.1"
                     /translation="MVAYLAGIFVLVSVLADWNRFFAHPRARFFVDILGRGGARLFYA
                     VLGSGLLFLGFCLQKP"
     gene            complement(5018..5464)
                     /locus_tag="VN496_13760"
     CDS             complement(5018..5464)
                     /locus_tag="VN496_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXF18070.1"
                     /translation="MANIIRRQDPFAGGTVSALSVRDPLDFITPLFGRVFGSVDNAGP
                     RLDVKESEQGYVLEMEIPGVDKNAITVSVYNDTVTVEAEVRQENDANTEYKWLHRERN
                     FGKISRTVALPEEVDEDAANARFENGVLQLTLPKKRSSAAKRLAVQ"
     gene            5691..6164
                     /locus_tag="VN496_13765"
     CDS             5691..6164
                     /locus_tag="VN496_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXF18071.1"
                     /translation="MDPLRTIARAWEELTEGWRQLLSRNSSALTHFVSGARKDPKAGS
                     APDFPQWGLLAAETWETAGTLIVRIEVPGMDKDDFDVSIHGNTLRIRGEKRSEDNHHE
                     RKYHLMERAYGRFERNFSLPQNIDRDKAEVSYRNGILTVILAKTEAIPPKQLPLQ"
     gene            complement(6184..6582)
                     /locus_tag="VN496_13770"
     CDS             complement(6184..6582)
                     /locus_tag="VN496_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF18072.1"
                     /translation="MPLLARLLITGTLLSVLSGALPMLSSSRAFGDELKNPFAGDPAA
                     AAEGKKLFLKTGCYACHGHEAEGAVGPDLTDDEWIYQPTDAMIFNTISRGRTGTVMAP
                     FGDQLKPDEIWKIVEWLRWKNRERKTSETK"
     gene            complement(6651..8348)
                     /locus_tag="VN496_13775"
     CDS             complement(6651..8348)
                     /locus_tag="VN496_13775"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXF18073.1"
                     /translation="MKPLSRHSIVACFITAFATLSLFSATALHAEVTNDMLLNAAKES
                     DNWRMVGRDYAGSRFSPLKQVNTKNIKRLVPKWTFSLGTLDAQNTTPLVSDGVMYVTA
                     AHGRTYAVDARTGTMIWQYAHQLPEDVGKAMCCDIGNRGAALYHDKVFVSTPDAHVVA
                     LDMKTGKKVWDVALGDYKLSYTMTVAPLIVKGNVVVGLSGAEYPTRLFIEALDAETGK
                     QAWRRYTIPAPGEPGSESWGADADNLKYGGASAWITGSYDPNTNLIYWGTGNPNPDWD
                     GVGREGDNLYSNSTLALNADTGEIQFHFQYTPHDVWDFDGVNENILADFDGEKVWLHG
                     DRNGYLYKIDRTNGKFRYGKEISKVNWSTGFSAEGRPIVNQEKVPTYDYEAKDICPAS
                     EGGKWWNPMSYSPEKKMVFVPSREICTDIKSAKGERAEGKPHWGIGSIKWNKGHGQLV
                     AFDARSGEKKWTVKAPSPFMSGVLTTGGSLVFAGTPEGEFKAYDQDTGKELWSYQTGS
                     GIVGSPISFSIGGKQYIAVPSGFGGWTGWATIGGGGAPHLKDSRKGGYVTVFGLFDE"
     gene            8571..>8968
                     /locus_tag="VN496_13780"
     CDS             8571..>8968
                     /locus_tag="VN496_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18074.1"
                     /translation="MRSSAMQYAKAALFGTVMLWSAGHGLCAADSSVPAIGAGELGGV
                     VKGGQGPEAGVWVIAETTDLPTRFAKIVVTDERGRYLIPDLPQATYNVWVRGYGLVDS
                     PRTRSVPGKRLDLAAVAAPSPAAAAQYFPA"
BASE COUNT         1787 a         2707 c         2611 g         1863 t
ORIGIN      
        1 cgttattttc cccgcgattg acggggcaac tctatcgtct cacgcgcggc gttccacgcg
       61 tgatcaacgt actatgtgat cgcgctctcc tgggcgctta cgtgcaaggc aaggaacgcg
      121 tcgatgccgc tacgctagcg caagcggcgc gtgaagtgtt tcaccagacg gacgaccgcc
      181 gcaatgtttt gcgcgcactc atcgctacct tgattctcac ggccggtgtc gccgtggcat
      241 ttaccctgca tcggcaggat atgaatgatg cgcgcatgtc catcccggta cgcggcagcg
      301 tgaagccggc ggcgtctccg agtcctgcga cggccgagcc aaccggcgca tcagtcgtgg
      361 cggccgctgc gacatccacg tcggcgttgg ccgattcgct ggagtggcgg gccggcgagg
      421 ggctcgcctc gaaccgtgcg caggcttttg cggtgctgct gaaggcgtgg ggtcaggcat
      481 tcgatcaaag tgcggacccc tgcaagcaag cagaaggcgc cggccttcgc tgccacagcg
      541 cgcgcggagg cttggacgag ttacgcgaac tgaaccggcc agccgtgctg cgaatgcgca
      601 acgctgccgg tgaagaattt gccgctacgt tgatggcgct tggcgagaag actgcgagct
      661 tcgcactagg cgaccaaagc gcaacgattt cgctcgatac cctggcatcg caatggtcag
      721 gtcattacac actgctatgg cgcatgccgc cgccacaagt gcgcgacagt attcgcccgg
      781 gtgaacgcgg tgccgccgtg caatggctcg cgggtcagct cgcccttgtg cgcgggctgt
      841 cagcgcctgc gtccaccgat ccggttttca atgaggaact gcagcgcgaa gtgaaaaaat
      901 ttcaactcgt acacggtctg attccggatg gcagcgtggg tccccaaaca ctgatgcacc
      961 tggcgagctg gggcgaccca acggcgccga agctttcttc aaagtagagc cgacaagcaa
     1021 gcatgtcctt cattctcgat gcgctacgca aatcggacca gcagcgccag cgcggcagcg
     1081 cgccaacatt gcttacagcg caggccgcag ccatcgggac caagcgacca tcgctgatgg
     1141 catatggggc gctcggcttg gtactgttgg cggccggcat cgtcattggc tggctgcggc
     1201 cgtggcagga gcaggtagca cctgtgccgc cctcaccgcc caagccgatc tcgacaccct
     1261 ctcggcctga acccgaaaca gcgcagcgtc ccagcgcagc gccatccgcg cccgctccac
     1321 aagctgccgt tccggacaaa tcaccatcgc ccggagctga tgcgattcca aagtcgagcg
     1381 gccagcagga agcggggacg ccagcatccg cgcaaacggt taaaacggtt cctgagcaga
     1441 ggacgaagca ggcgaccgag aagccgcgcg caccgctgtc gcaggcggcc acaatatcaa
     1501 gcagaccgcc acagccggcg caggtgccag ctgttaatgc agatggctcg agcggcgctt
     1561 cagaacaacc gccgccctgg atgactgaac tgccagtcga agtccagcgg gacctgccag
     1621 ccatgcacgt catggtgcat gcctattccg cacggccgac ggaccggctg gtcgggatca
     1681 ataacaagct gttgcatgaa ggcgatgaag tcgcgccagg tctaaagctg gaacagatca
     1741 cgcccgaagg catgatcctg aacttcaagg gccaggtttt ccgccgcggc gtgcactagg
     1801 gcaaaacgac tcccgccggt gcgttgatcg aaatcgaatt ggcagcaagc cgtgccaacc
     1861 tgtatgtcat ggtttgcccg ccggcgtcaa cgccgactgc ccagccataa ccctgagcgt
     1921 tgtaatggac aatccatgcg acgcaagctt gcggcttagt gcttgctcca tcgctgcggc
     1981 tgacacgcgc gtcggatcga aggctgcaga cactgccgcg ggagcgccgg atacgcgggt
     2041 actgcctttg tctactccgc taagggattc caccgccgtt ttcagcgcgc gccgcaattt
     2101 ttcattcggg acgatcgttc cttctaccgc gaagaacacg acctgatggt gacgtgagag
     2161 tgcgtgcgtc accacgccgt agtcataaac caccgcaatc ttgtcttcga tgcagtagcc
     2221 gcacgccaaa gccgtggatg tgaggcccag cagaatcatt gcgccgaagt gatggcaccg
     2281 cgcgcgaatc accgtttgcc ctcctttgcg tcgtgcccgc cggtggcgtt ttgctcatct
     2341 gcccacttga aatatttgat gtactgctgg acttccgtgt cgctcagatt ctgattcggc
     2401 atgggcagtc caccggcttc cttcagcatc gcctttgcat ccgcgtccac ctccagcatc
     2461 ttttccggcg tcttgagcca ctttgtcagc cattcattgc tgcggcgctt ggtcacgccc
     2521 gccaaatccg ggccgagctt gcgcccctgg ccgatcgaat ggcaggccag gcatttactc
     2581 tcaaaatcga gcttgccttt gaccgcttcg gggtcaccgg gtgtgcgcgt ggcgggaata
     2641 ttgtgatgtt cgatctcgcc cttctcttcc ggtggtccgc ctgcgtcgat cagaccgatg
     2701 gcaccctgtg aggcattcgc gaaatggtgg tcgaccatga tgtactggcc tgcttcaggg
     2761 atgacgaact caacaatcgc cccgctggat gaaccaagca gcacggtttg catgccgcgc
     2821 aactgattgt ccggattacc gtccagccag acgcgatcga agatggtgcc caccacatgg
     2881 aagctggatg tcttgctggg gccgacatta agcacgaaca gacgcacgcg ctcacctggc
     2941 tttgccgcca gcggcttctt caccatgccg ttgtgcacgc cattgaatac ggtatagccc
     3001 ggcgaggcag cgcgcaggcg ctcgccgtcc agcacataca gcggggcgcc atcgatgctg
     3061 cgtttttgcg ggtccagctt cggatagaac tcgctctgga tgaccacgta ctcgcgatcg
     3121 actttggttg gatagccttc gcgcggctcc accacaattg cgccatacat gcccgaagca
     3181 atgtgctcca gaatcattgg cgtgccgcag tggtacatga acacgcccgg gtagttcagc
     3241 gtgaactcga acgtgatggt ctgccccgga gcgatggaat gatatttgtc ctgcggagaa
     3301 accatcgccg cgtgaaaatc catggagtgc atcatcggcg ggatcatcat ctgcaagcca
     3361 ggcgcgactt cgtcactgcg gttggtcatg ctgaatttga tcttgtcgcc tacgcgcgcg
     3421 cggatcaccg gtccggggac ctgatcgccg aaggtccagc cgctgaaccg gatgccaggc
     3481 gcaatctcga ttattttgtg cgtggtatcg agttgaatgg ttttcaccgg cgacgcttcc
     3541 acaggcttta tatcgggagc cgtactaagg gcaacgcctg aggagaaagc cccggtgtgc
     3601 ctctcgctgg acattttgga aggcagcggc ttcaaatcca ttttcgcctg atcgtaacga
     3661 tccggaggcg cactttggtt gcatgcggcc agtcccagta ccagagccgt gcaagccatg
     3721 ctgtgcagtg acaaaaatgc tggtctttgg aaacgcatgt ggatcgatac tttcatgctg
     3781 gttctccccg tcgtctctgc tcaaaggtgc gcagcgtgtt tatcattgtt tggcgaaata
     3841 cctgtgccag attttcgtgg gtgacagttg aactgcagct ccaggagatg agatttgtaa
     3901 ttcgttgcct tgcgttccgt gatatgccgg cacttgctat tcgggcaaag ctccggcggc
     3961 ccatagaatg ccgccggaca aatgctcgag gacaagcggt tcacggtagg attcgacggt
     4021 atggccaatt gcggtgtacc agccgcgccc gccatcatat tgatgatgcc acgctatcgg
     4081 atggtcagcg cccatccggc cgccacgata cgttgcctct tcgatacgta tcaaaacggt
     4141 gatgtctgga tcgagtgcgt ggcggaagtc ataccactcg tccgttctct tccaggtgat
     4201 gggcagatgg cgagtcgacg cgtgagacgc atcgaccacc ttcagcgttg caggctgaat
     4261 cgccggatgc cgctggaaat aggttcccac cagtccgcca taccacggcc actggtaccc
     4321 ggtgtcgctt gcggcatgga tgccgacgaa tccgccgccg tgctgaatga accgttcgaa
     4381 cgccgcgcgc tggtcggctt ccagaatttc cccggtcgtg ttcaggaaaa tcacggcccg
     4441 gtaccgcgcc agattggcat cggtaaatgc gccagcgtct tcgcttgcgt ccacgcgaaa
     4501 accatttcgt tcgcctagca tgtggatggc atcgatgcct gccggaatgg aatcgtggcg
     4561 gaagcctgca gtcttcgaga aaagaagcac agcgaaaccg cgttctggtt cggcagattg
     4621 cgaatgagct tgccctaata cagctagtag aacaccggcc atggcagaga atgtttttgc
     4681 agcgtccatg cgattcaagg tttctgaaga caaaagccca gaaacaatag tccactcccc
     4741 agcacggcgt aaaacaggcg tgcgccaccg cgtcccagaa tatcgacgaa gaacctggcg
     4801 cgcggatgtg cgaagaaccg gttccagtcg gccaggacgg acacgagcac gaaaattccc
     4861 gccagatacg cgaccactgc caaggctttg cctcaccgac gcaattcagc gccatgagca
     4921 taccgcattg gttggactcc cgagcacgcg cagcgccgcg ggaacgatgg gcaaaaagaa
     4981 gcccgccgga cgacgggctg gtagatgcgg ggagtgctta ctgaacggcc agacgtttgg
     5041 ctgcgctgga acgcttcttg ggaagggtca actgaagtac accgttttcg aatcttgcat
     5101 ttgcggcgtc ctcgtccact tcctcaggca gcgcaaccgt tctcgatatc tttccgaaat
     5161 tgcgttcgcg atgcagccac ttgtactccg tgttcgcatc gttttcctga cgcacttcgg
     5221 cttccacggt aaccgtgtcg ttgtatacgc tgaccgtgat cgcgttcttg tccacccccg
     5281 gaatttccat ttccagcaca tacccctgct cgctttcctt gacatccagg cgcgggccgg
     5341 cattgtcaac gctgccaaac acgcgaccga acagcggcgt aatgaagtcg agcggatccc
     5401 tgacgctcag ggcggaaaca gtcccgccag cgaaggggtc ctggcgacga ataatatttg
     5461 ccatgattga tttctccaat accagatgta gtcagaattc ggcaggcgtt caacctgctc
     5521 cgtcaaaata agggcggcca tcggtttttc aacagccgca gcaccgaccg aatactccag
     5581 tgcccgcgcc ggacgccttc tgatcgtttc cgcttgagtg ggcgcttgaa ttccggcgag
     5641 tccggacaaa tatcttttgt tatcgcacgg acgaccgact ggggcggacc atggatccac
     5701 tcagaaccat agcgagggcc tgggaagaac tcaccgaggg ctggcgccag ttattgagtc
     5761 gaaacagcag tgctctgact cactttgtca gcggtgccag aaaagacccg aaagccggtt
     5821 cagcaccgga ttttccgcaa tggggcttgc tcgctgccga aacctgggaa acagctggca
     5881 cgctgatcgt ccgcatcgaa gtacccggca tggacaagga cgatttcgat gtctccattc
     5941 acggcaacac actgcgcatc cgtggcgaaa agcggtccga ggataaccat catgagcgca
     6001 aatatcatct gatggagcgc gcttatggcc gtttcgagcg caatttctcg ctgccgcaga
     6061 acatcgaccg ggacaaagcc gaagtatcgt accggaacgg cattctgacg gtgatactgg
     6121 cgaagacgga agcgattccg ccaaagcagt tgccgcttca gtaaatgtca gagaagcggc
     6181 gccctacttt gtttcggacg ttttgcgctc acgatttttc cagcgcagcc attccacgat
     6241 tttccagatc tcgtcgggct tgagctgatc gccgaaaggc gccatcaccg tgccggtcct
     6301 gccgcggctg atcgtattga agatcatggc gtcggtcggt tggtagatcc attcatcgtc
     6361 tgtcaggtcg ggaccgaccg ccccctcggc ttcatgtccg tggcatgcat agcaacccgt
     6421 tttcaaaaac aactttttgc cttccgcggc tgctgccgga tcgccggcga acggattttt
     6481 cagctcatca ccgaaggcgc ggcttgacga cagcatcggc agcgcgccgc tcaggacact
     6541 gagcagtgtc cccgtgatca acaagcgggc aagcagtggc attggtgtat gcgtcgaaga
     6601 cgaaattgcg ggaggccgca gtttgctgcc cctcccgcca cggctttgga ttattcgtcg
     6661 aacagaccga acaccgtcac gtagccgcct ttgcggctgt ctttcagatg cggcgctccg
     6721 cctccgccga ttgtggccca gccagtccag ccgccgaaac ccgagggcac cgcaatgtac
     6781 tgcttgccgc cgatggaaaa gctgatcggg ctgccgacaa tgcccgagcc ggtttggtag
     6841 gaccagagtt ccttgccggt atcctggtcg taagccttga actcgccttc cggcgtaccc
     6901 gcgaatacaa ggctgccgcc ggtggtcaat acgccgctca tgaagggcga cggtgccttg
     6961 acggtccact tcttctcacc gcttctcgca tcgaaggcaa ccaactgacc gtgtcccttg
     7021 ttccatttga tgctgccaat gccccagtgt ggcttgcctt cggcgcgctc gccttttgcg
     7081 ctcttgatgt cggtgcagat ttcccggctc ggcacgaaca ccattttctt ttccgggctg
     7141 tagctcatcg ggttccacca cttgccgcct tcggacgccg ggcaaatatc cttggcctcg
     7201 tagtcgtagg tgggcacttt ctcctgattg acgatcggac gtccttcggc cgagaagccg
     7261 gtcgaccagt tcaccttcga gatttccttg ccgtacctga attttccgtt ggtgcggtca
     7321 atcttgtaca gatagccgtt gcggtcgccg tgcagccaca ctttttcgcc gtcgaaatcg
     7381 gcgaggatgt tttcattgac gccatcgaag tcccatacgt cgtgcggcgt gtattgaaaa
     7441 tggaactgga tttcgccggt atccgcgttt aatgccagcg tggaattgga gtacaggtta
     7501 tcgccttcgc gtccgacccc gtcccaatcc ggattcggat tgccggtgcc ccagtaaata
     7561 agattggtgt tcggatcata ggaaccggtg atccaggctg acgcgccgcc atacttcaga
     7621 ttgtcggcat cggcgcccca gctttccgaa cccggctccc ccggcgcggg gatggtgtaa
     7681 cgtcgccacg cctgcttgcc cgtctctgca tccagagctt caatgaacag gcgggtcgga
     7741 tattccgcac ccgacaatcc gaccacaacg ttgcctttga cgatgagcgg agcgacagtc
     7801 atcgtgtaag acagcttgta atcgccgaga gccacgtccc acactttctt gcctgtcttc
     7861 atgtcgagcg cgaccacatg cgcgtccggc gtcgatacga aaaccttgtc gtgatacagc
     7921 gcggcgccgc gattgccgat atcgcagcac atggccttgc ccacgtcttc cggcaactga
     7981 tgcgcgtact gccagatcat ggttcccgtc ctggcatcaa cggcataggt ccggccatgc
     8041 gcggccgtca cgtacatcac gccgtcactg accagcggcg tcgtattctg cgcgtccagc
     8101 gtgcccagcg aaaaagtcca cttcggaacc aggcgtttga tatttttggt attgacctgc
     8161 ttcaacggac tgaagcgcga gcccgcatag tcccgtccaa ccatgcgcca gttgtcggat
     8221 tccttggccg cgttcagcag catgtcgttc gtgacttcgg cgtgaagggc ggttgcgctg
     8281 aagagcgata aggtcgcaaa agccgtgatg aagcatgcaa ctatagaatg ccgtgaaagt
     8341 ggtttcatga attccccttg agagatggcg gatgcagtgc tgcggcggct cgcagttttc
     8401 gtccgtgcga tggcgccgca cagatactac cgtctttgaa aatgggcgta atgcttttcc
     8461 gccgcgcgcc gatatcgggc gccgtgtggc accgaacagg tgcactcgcg gcgcggcaaa
     8521 tcatcttaaa ctggcggctg gctagaagaa gaaccaaagg agcaggcgag atgagaagct
     8581 cagcgatgca atacgccaag gcggctttgt ttggcacggt catgttgtgg agtgccggac
     8641 acggcttgtg cgccgccgat agttcagtgc ccgcaatcgg cgccggcgaa ctgggcggcg
     8701 tggtgaaagg cgggcagggt ccggaagccg gtgtctgggt catcgcggag acgaccgacc
     8761 tgccgacccg tttcgcgaaa atcgtggtca ccgacgagcg cggtcgctat ctgatcccgg
     8821 atctgccgca agcgacttac aacgtatggg tgcgcggcta tgggctggtc gactccccga
     8881 gaacgcgcag cgttccgggc aaacgcctgg atctcgcagc tgtggccgca cccagtcctg
     8941 cagcagcggc gcagtacttt ccagcgat
//
LOCUS       DATMZQ010000431         2929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5009388, whole genome shotgun sequence.
ACCESSION   DATMZQ010000431 DATMZQ010000000
VERSION     DATMZQ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5009388"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VN496_13785"
     CDS             complement(<1..1104)
                     /locus_tag="VN496_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF18075.1"
                     /translation="MWIVRLALGRPYTFVVIAMLIVLIGVVSILRMPKDIFPEISEPI
                     VTLIWQYVGIPPDAFEKQITIFSEQQISTTVSNVKRIESHTINGKNVIRIYFQPSVRI
                     DQAVSQITATSQTIIRRMPPGQQPPQIVQYDASSVPIMQIALGSDTLSEQQLYDQGLW
                     LVRNEVVPVPGATVPLPYGGRPRLVMIDADPVRMHAKGLTVKDINDALNRQNVNLPTG
                     STQIGSRDYILSINNSPEKISELGLLPVKSVGGTMVYINDVADVRDGYNEQTSLVRHD
                     GRKSALITVLKSGGASTLDIIREVRQRLSNVTLPEGLSLSFLFDQSVFVASAMHAVVV
                     EGLMAAALTGLLILLFLGSWRSTVVVVTSIPLAI"
     gene            1352..2551
                     /locus_tag="VN496_13790"
     CDS             1352..2551
                     /locus_tag="VN496_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF18076.1"
                     /translation="MRGQISGIALVIGLAAVMAGLLAIGLWPKWNALKLAQAEAGKDD
                     LPAVMYVAAKRGGQKSDIVLPASLIAMQETTIYARTNGYLKRWLVDIGDQVKAGQVLA
                     EIEAPEVDRELDQSHAARAQAKANLDLARVTAERYGSLLKEEAVSPQEVDEKYGVHQA
                     RKADYAAAEANLRRLEQMKSFQRVTAPFSGTIVARNVDVGSLIQAGSASSSGWLFKLS
                     QTDTIRVHAAVPQSSMRMIKPGMTVDVVVPELGERAFAAKVMRNAGAFDAATRTMVVE
                     MHIPNRDGPLLPGMYAQAKVSLVNAEPNLQIPINALMIGGDGTRVAVVDTSGVIHIKP
                     VKLGRDFGKEVEILTGLADNDRVINNPRDHLLEGMKVRATEMQVHGEKKAPAPPTAAP
                     ATEKSKS"
     gene            2561..>2929
                     /locus_tag="VN496_13795"
     CDS             2561..>2929
                     /locus_tag="VN496_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HXF18077.1"
                     /translation="MGTRRVAAIAFAMLVTGCTHVGPDYVRPKATFPPAFIEPGPWKE
                     ATPSDESIRGKWWEIYADPVLNRLEEQARSSSPRVQAAAARVAQARAVLGFNQAGAYP
                     TLDFSPDASRYRVSGNRPDQP"
BASE COUNT          649 a          826 c          858 g          596 t
ORIGIN      
        1 gatcgccagc ggaatcgatg tgaccaccac taccgtggaa cgccagctgc cgaggaacag
       61 cagaatcaac agaccggtca aggctgccgc catcaatcct tcgaccacga ccgcatgcat
      121 ggcactggcg acaaatacgg actgatcgaa caggaaagac agcgacaagc cttccggcag
      181 cgtcacattg gacaagcgct gacggacttc acgaatgata tcgagagtgg atgcaccgcc
      241 gctcttgagt accgtgatca gcgcagactt gcgaccgtca tggcgtacta gactggtctg
      301 ctcgttgtaa ccatcccgca catcagcgac gtcgttgatg tagaccatgg tgccgccgac
      361 gcttttcact ggcagcaatc cgagttcgct gatcttttcg gggctgttgt tgatgctcag
      421 gatgtagtcg cgcgaaccga tctgcgtact gccggtcggc aggtttacgt tctgcctgtt
      481 gagcgcgtcg ttgatgtcct tgaccgtgag gcccttggcg tgcatgcgca ccgggtcggc
      541 atcgatcatc accagtctgg ggcgtccgcc atatggcagg ggcacagtcg ccccgggaac
      601 cggtaccacc tcgttgcgga ccagccacaa gccctgatcg taaagctgct gttcgctcag
      661 cgtgtcgctg cccaacgcga tctgcatgat cggaacgctc gacgcgtcgt actgcacgat
      721 ctgtggtggt tgctggccag gcggcatgcg ccggatgatc gtctgcgaag tcgcagtgat
      781 ctgactgact gcctggtcga tccgcactga aggctggaag tagatgcgga tgacgttctt
      841 gccgttgatc gtgtgggact cgatccgttt gacgttactg acagtcgtgc tgatctgctg
      901 ctctgaaaaa atcgtgatct gcttctcgaa agcatcgggt gggatgccga cgtactgcca
      961 gatcaacgtg acgatcggtt cggagatttc gggaaaaata tccttcggca tgcgaaggat
     1021 ggatacgacg ccgatcaata cgatcaacat cgcgatgacc acgaaggtat agggccggcc
     1081 aagcgccagc ctgacaatcc acaacgctct ctcccctgtc agcgttttgt tgcaacaaca
     1141 ttactttaat gttacaagtt tatgcgtggc cgttacaaga aaatctgata taaatcagat
     1201 atttcaaact gaaacctata tttcaaaatg aaatattttg cgatgcaacc gaacagaaac
     1261 tgtcgggagg aacgcacaaa tgaggacgtt ttcacgaatg ctttttgcac actgcaacat
     1321 ctcctatggg cgtcccattg gccttcgaaa catgcgcggc caaatctcgg gtatcgccct
     1381 tgtcattggg ctcgctgctg tcatggccgg tcttctcgcg atcggtctgt ggccgaaatg
     1441 gaatgcgttg aagctcgcgc aggccgaagc cggaaaggat gaccttccgg ccgtgatgta
     1501 tgtggccgcc aagcgcggag gccagaagtc cgacatcgtg ctgcccgcaa gcctcatcgc
     1561 aatgcaggaa acgactatct atgcgcgaac gaacggttat ctaaagcgct ggctggtcga
     1621 catcggcgat caggtcaagg ctggacaggt gctggccgag atcgaggcgc cggaagtcga
     1681 tcgcgagttg gaccagtcgc atgccgctcg tgcgcaagcc aaggccaatc tcgatcttgc
     1741 gcgagtcacc gcggagcgct atgggtcgct gctaaaggag gaagctgttt cgccgcagga
     1801 ggtcgacgag aaatacggcg ttcaccaggc gcgtaaggct gactatgccg ccgcggaagc
     1861 caatctgcgg cggttggaac aaatgaaatc gttccagcgc gtcactgcac ctttctccgg
     1921 gaccatcgtc gcacgcaatg tcgatgtcgg ttcattgatc caggccggca gcgcctcatc
     1981 ttctggctgg ttattcaaac tttcgcaaac ggacacgatt cgggtgcatg cagccgttcc
     2041 acagagcagc atgcgcatga tcaaacccgg catgaccgtt gacgtggttg tacccgaact
     2101 cggcgaacgt gccttcgcgg caaaggtgat gcgcaacgcg ggcgctttcg atgcggccac
     2161 gcgaacgatg gtggtcgaaa tgcatattcc aaatcgcgac gggccgctgt tgccaggcat
     2221 gtacgcacag gcgaaggtga gccttgtcaa tgccgagccc aatttgcaga ttccgatcaa
     2281 tgcgctgatg atcgggggag acggcactcg cgtcgcggtt gtggatacca gcggtgtaat
     2341 ccacataaag ccggtcaagc tcggtagaga ctttggcaag gaggtggaaa tcctgaccgg
     2401 cctggcggac aacgaccgcg taatcaacaa tccgcgcgac cacctcctcg aaggcatgaa
     2461 ggtacgcgca acggaaatgc aggttcatgg ggagaagaaa gcaccggcac cgccgacggc
     2521 tgctcccgct accgaaaaat ccaaatcctg acccgccgtg atgggaacgc gccgtgtagc
     2581 tgcgatcgct tttgcaatgc tcgttacggg ttgcacgcac gtcggccccg actacgtacg
     2641 tccgaaagcc acatttccac ccgcgttcat tgagcccggg ccgtggaagg aggccacgcc
     2701 gagcgatgaa tccattcgcg gaaaatggtg ggaaatttat gccgatccgg tactcaacag
     2761 gctggaagag caagccaggt cgtctagccc tcgcgtgcaa gcagccgcgg cgagagtggc
     2821 gcaggctcgc gccgtgctgg gtttcaacca ggcaggggct tatcccacgc tcgatttttc
     2881 tccagacgcg tcgcgttatc gcgtatcggg caatcgtccc gaccagcct
//
LOCUS       DATMZQ010000432        10847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4667550, whole genome shotgun sequence.
ACCESSION   DATMZQ010000432 DATMZQ010000000
VERSION     DATMZQ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4667550"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1221
                     /locus_tag="VN496_13800"
     CDS             247..1221
                     /locus_tag="VN496_13800"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXF18078.1"
                     /translation="MAEQGNWAFPKEVQPRQDELRFELDPVLDAVVQLRSEVPDDAFT
                     ATILGTERAGNGIVIGDDGLVLTIGYLITEARSLWLTTNAGIAVAGHALAYDQVTGFG
                     LVQPLGRLKASPLRRGSTSACAIGDSVIVISHGGPAHALKAKIIARREFSGYWEYLLD
                     EALFTAPAHPQWGGAAVVDMDGRLLGIGSLLVEEAYEGKQAQGNMIVPIDLLEPILDD
                     MTKLGRPARAPRPWLGMYAAETDGHIIVGGLATDGPADRAGIRQGDLVLEVAGQRVAS
                     LADLFRKVWGLGPAGTEIPMTLAREGSQLSVRLQSIDRNDMLKKPLLH"
     gene            complement(1222..3003)
                     /locus_tag="VN496_13805"
     CDS             complement(1222..3003)
                     /locus_tag="VN496_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXF18079.1"
                     /translation="MAAFVLASDVLVVLCSVVWRYFLHDPVDWAEEIARALMGMQVFL
                     GAATTLARAQHIGIDSFRALFPASWRPLLVQLCHWIIVVVAIALFASSCSLVLDSSGQ
                     TTPIGLPQWIYVGPIVIGSALMLVFGIAAALAGPRKGVWGTLAVAAMIAALVWIWNTA
                     MPAQAVTPLMLLATGFVLSIVAGLPIAFALAFSALLYFLADPSLPMLVYSQQVLSGMD
                     HFVLLAIPFFVLAGLLMEANGMSSRLIELLLRMMGRVRGGLSLIIITATAFFSGVSGS
                     KLADVAAVGGIIMPAVRRTRQDPNDAAGLLAASAVMAETIPPCINMIIMGFVANISIG
                     GLFVAGLVPAAFMALSLALVAFAFGSRIDPDAAFEQRRNIVPLVGGALVGLVMIAMIG
                     KGVTSGIATSTEVSAFAVIYALFVGAAAFRELSVRSVLLLFMRSASMAGSILFIVAAA
                     SSVSFALTIQQIPQQLSGFMMDLGHRFGANGFVLASTLLMICFGAILEGAPALIIFGP
                     LLTPIALQLGVSPLHFGTVMVVAMGFGLFSPPVGLGLFATCSITGTEIHQVIRPMMKY
                     LAVLFLALLALVFVPAFSLWLPARFGF"
     gene            complement(3115..4143)
                     /locus_tag="VN496_13810"
     CDS             complement(3115..4143)
                     /locus_tag="VN496_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXF18080.1"
                     /translation="MTTSGSRRRFLAAGTASAALFSAGIPIAAKAQASVPLRISSSLT
                     ADENSAHYIWFQRFADNLRAGTGSRIAPDFFPNSQLGKEADVVQQVKVGSIDIMLTGS
                     SIWATVAPEFGMLDLGYIFDSYAHMARALDGGVGGNLEELLQKRTACIVLGWGSHFAA
                     RSVYTKAPITSRAALKGVKLRVLPTSAFIDTFKLMGAVPTPIPFNELYTAVQTGVVEG
                     FEHDASTVLATKLYEVTRHCFLTEHLFPPMIAVIGKRGFDKIPADIRPAFLRAAQEAS
                     LYQRAVAGEKGRSAFDKLAKLGLSFHAMAKDERSALRTEMETRLWPAFAEQYPETKPL
                     FKAIAAAR"
     gene            complement(4172..5428)
                     /locus_tag="VN496_13815"
     CDS             complement(4172..5428)
                     /locus_tag="VN496_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF18081.1"
                     /translation="MENKRNNRQVARGGVDTSYAWTRLVLALLLSTIGGVGMWSVIVA
                     LPAVQAEFGVARGDASLAYTLTMLGFGAGGIVLGRMTDRRGIVPPVMVGIVCLIFGYA
                     AAASASTLWQYALAHGLLIGFGSSATFSPLLADTSHWFERRRGIAVSIIASGNYLAGT
                     LWPPIVQHFIESNGWRPTFLGIAIFCAATMFPLVLALRKPVPSTDEPVTPATSDHANR
                     SRPLGLSPQSLQTLLIVAGVCCCVAMSMPQVHLVAYCGDLGYGAARGAQMLSLLLGCG
                     IVSRLVFGMVSDRIGGLRTLLIGSTMQCFALFLFLPFDGLASLYVISALFGLFQGGIV
                     PSYAMIIREYFSPKEAGTRIGIVLTATMVGMALGGWMSGIIFDVTGSYRMAFLNGIAW
                     NLVNMAIALMLLVRSRRSPVRASVGA"
     gene            5582..6082
                     /locus_tag="VN496_13820"
     CDS             5582..6082
                     /locus_tag="VN496_13820"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXF18082.1"
                     /translation="MKKLSTAIACGLMLAQLNAFAFEAVARVVAVSPIMQQVNQPSQQ
                     CWTETQPVTQAAPQQHNYVGAIVGGVAGGLLGAQVGRGNGRVAGAAVGAGVGAVAGDA
                     VANSSNGTVTTMTPVQRCRQVEKVQSVTTGYMVTYEYDGQRFSTRLPYNPGNQLRVNV
                     AVTPHQ"
     gene            6186..6956
                     /locus_tag="VN496_13825"
     CDS             6186..6956
                     /locus_tag="VN496_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18083.1"
                     /translation="MKLEGSVCIVTGSATGTGAACAIHLAKKGCRVVVNYTKSEKEAV
                     QTLDSCKAAGTDAIMVKADVAADMDCRALAKAALDKWGRIDALINNAGITKFAAAADL
                     DGLDAGDFQRLYAVNVIGPYQMIRACAPVMKRQGKGAVVNISSISGVMGIGSSTAYVA
                     SKGALNAMTLALARSLAPEIRVNAVCPGLIETRWHLSRFSKEDYAKFKAGYEKTVPLA
                     KAASPEDVAEVAVWLIEGADLVTGESILVDAGLHLGKF"
     gene            7154..7489
                     /locus_tag="VN496_13830"
     CDS             7154..7489
                     /locus_tag="VN496_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18084.1"
                     /translation="MRILIPALAAASILMISLPSYSADDVKSGGTSTKPARALDDGAV
                     KSGGDTNKPGRAVEDNSVKAAGATSKPGRAVEEGTVKSGGDTTKPARAVEGDKKAAKK
                     KKKKKVETQ"
     gene            complement(7615..8238)
                     /locus_tag="VN496_13835"
     CDS             complement(7615..8238)
                     /locus_tag="VN496_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF18085.1"
                     /translation="MKILHADDHSLFREGLGFFLKLLHAQAVALEASNLQSALDKLAL
                     EWPVDLLLLDLEMPGMNGLEGFYTIRRRYPNLPVAIVSGVNDARIIRQLIDGGARGYI
                     PKLAKSEQLMDALRRILQGEVYLPEALFIPASGSAAGGQMSALTSRQQQILPLLAEGM
                     PNKQIADVLGVTEGTIKQHLKDLFRRLEADNRTQAVKQAQRLGWLKK"
     gene            complement(8309..9430)
                     /locus_tag="VN496_13840"
     CDS             complement(8309..9430)
                     /locus_tag="VN496_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXF18086.1"
                     /translation="MKQAVMTLPAERREATLLIVDDKPQNLRLISDFLAEQGFELMLT
                     RSGAQALEKIQRSMPDLVLLDVTMPEMDGFEVCRRLKIEPRTASVPVIFMTALDDTAH
                     KVQGFRLGAVDYITKPIQREELLARIQHHLQLHTLQKELRAKSDDLAQKNAELEAYAH
                     TIAHSLKTPLAAACRFLEILQKFKSDGLAAEQRDLLQKSSSALAMTGEAVDALLLLST
                     VAQQNVEMHPVDMNSVVVQVLSQLADLQSRTGAQVKLPDTWPSALGYEPWVGEVWLNL
                     VSNALKYGGSPAQVELGGASEGSQVRFWVRDNGQTLSEHERRRVFIPFTRLHQERAPG
                     HGLGLATVQRILSRLGGNVGVRPGPQLGNEFFFELPAAP"
     gene            complement(9399..>10847)
                     /locus_tag="VN496_13845"
     CDS             complement(9399..>10847)
                     /locus_tag="VN496_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF18087.1"
                     /translation="QRKALLAARAEAEAANRSKDQFLANMSHELRTPLHAILGYADLA
                     GGGTMPDQSRREALATIASSGRHLLSLINDLLDLSRIRSGHLELNPAPVQLAALFEEI
                     AAMVRVEAHRKGLDFFLEIAAGLPDTVEADGKRLRQILLNLLGNAIKFTDSGGVTLSV
                     QCVPKEIGSVELRVSVRDTGAGISPKDTSRIFAPFEQAEEGHKQESGVGLGLAITREL
                     ARLMGGDVGVDSQLGRGSQFRFTVKLPIAQTQHDVASLDERIVGYSGVRRSILVVDDQ
                     EENRRLLKQLLEPLGFEVMLADGGQEALTRARAHRPDLIVMDLRMPGMDGMEAARAIR
                     NTPGLEHIAIVAASASSADLDRAEADPVTFIECLRKPFRTSELLDAIQASLALNWRYL
                     EAELPAAGPAKEDNGETVLPPRAALEQLLDLARMGKLVRVEQMASDLKNQDARYASFS
                     QRLYTLARGFEEERLVAMLEDCLETSRDDVAG"
BASE COUNT         2439 a         3212 c         3113 g         2083 t
ORIGIN      
        1 gctgtcacac cagatcggcg cttttctcgg cgcatggctg ggaggcctcg cggtcgtcaa
       61 tcttggcgac tacacggtta tgtggtatct cgatgcggga ctggcggcgc tcgccgcgct
      121 tgtgaatgtg ccgatccgtg aagcggtcat cgtaccgaag ctgcagcccg tctgaacctt
      181 tacgctttcg gcgaatcgcg taacatgccc gctggtcttt gaggcggacg caattcgaca
      241 cgacgtatgg cagaacaggg caattgggct tttccaaaag aagtgcaacc gcggcaggac
      301 gaactccgtt tcgagcttga tcccgtgctc gatgccgtgg ttcagctgcg ctcggaagtc
      361 cccgacgatg cgtttacagc gaccattctg gggaccgaac gggcgggcaa cggtatcgtc
      421 atcggtgatg atggcctcgt gttgacaatc ggttatctga tcaccgaagc gcgaagcctt
      481 tggcttacga ccaatgcagg cattgcagtg gcgggccacg cgcttgcata cgaccaggtc
      541 accggatttg gattggtgca gccgctcgga cgcctgaagg catccccgct tcgtcgtggc
      601 agtaccagcg cttgtgccat cggcgacagc gtgatcgtaa tcagtcacgg cgggcccgcg
      661 cacgcgctaa aagcaaaaat cattgcgcgg cgcgagtttt ccggctattg ggaatacctg
      721 ctcgatgaag cactgttcac cgccccggct catccgcaat ggggcggcgc ggcggtcgtg
      781 gacatggatg ggcggttgct tggcatcggg tccttgctgg tggaggaggc ctacgagggc
      841 aagcaggcac aaggaaacat gatcgtgccg atcgatctgt tggaaccgat cctggatgac
      901 atgaccaaac tcgggcgccc ggcacgtgcg ccgcgtccct ggctgggaat gtatgcagcg
      961 gaaacggacg gccatatcat tgtgggcgga ttggccactg atggtcccgc ggatcgcgcc
     1021 ggcattcgtc aaggcgatct ggtgctggaa gtcgctggcc agcgcgttgc gtcgcttgcc
     1081 gacctgttcc gcaaggtctg gggtctcggt cctgccggta cggaaattcc catgaccttg
     1141 gctcgcgaag gctcgcagct cagcgtgcga ctgcagtcga tcgatcgcaa cgacatgctg
     1201 aaaaaacctc tgctgcacta gctaaaaacc gaaccgcgca ggcaaccaca gcgaaaatgc
     1261 tggtacgaat accagcgcca gcagcgcaag gaacagcacg gccagatact tcatcatcgg
     1321 ccggatcacc tgatggatct cggtcccggt aatcgagcat gtggcgaaaa ggcccaagcc
     1381 gaccggcggg gaaaacaacc cgaatcccat cgcgaccacc atgaccgtgc cgaagtgcag
     1441 cggcgataca cccagttgca gcgcaatcgg cgttagcagc ggcccaaaga tgatcaatgc
     1501 cggggccccc tccagaatgg ccccgaagca gatcatcagc aacgtcgatg ccagtacgaa
     1561 cccattggca ccaaagcggt gaccaaggtc catcatgaag cccgacagtt gctgcggaat
     1621 ctgctggatt gtcagcgcga aggacacgct ggaagcggca gcaacaatga acaggatgct
     1681 gcctgccatt gaggccgaac gcatgaacaa tagcagcacg ctgcgcacgc tgagttcccg
     1741 gaacgcggcc gcaccgacga atagcgcata gatgaccgca aaggcggaca cttcggtcga
     1801 agtcgcgatg ccggacgtaa cgccctttcc gatcatcgcg atcatcacca agccgaccaa
     1861 cgcccccccg accaggggca cgatgtttcg gcgctgttcg aaggccgcat cgggatcgat
     1921 gcgcgagccg aacgcaaagg ccacgagcgc cagcgacaat gccatgaacg cagcgggcac
     1981 caggccggca acgaatagtc cgccgatgga aatgttcgct acgaagccca tgatgatcat
     2041 gttgatgcat ggcggaatgg tttccgccat gaccgccgag gcagccagca gtccggcagc
     2101 atcgttcgga tcctgccggg tgcggcgcac ggcgggcatg atgatgccgc caactgccgc
     2161 gacatcagcg agcttcgacc cggagacacc ggaaaaaaaa gcggtcgcgg taatgatgat
     2221 caggctcaac ccgccgcgca cacgccccat catgcgcaac agcagttcga tcagcctcga
     2281 cgacatgccg ttcgcttcca tcagcaggcc cgccagcaca aaaaacggga tcgcaagcaa
     2341 tacgaaatga tccatgccgg acagcacttg ctgtgaatac accaacatgg gcagcgatgg
     2401 gtccgccaga aaatacagca gcgcggagaa tgccaaagca aaggcaatcg gcaggccggc
     2461 cacaatgctg agcacgaaac cggttgccaa tagcatcaag ggtgtaaccg cctgtgcagg
     2521 catggcagtg ttccatatcc agaccagcgc ggcgatcatc gctgccacgg ccagcgtgcc
     2581 ccacacgcct tttcgcggac ccgccagcgc cgcagctatt ccaaacacca gcatcagcgc
     2641 gctgccaatg actattggac cgacatagat ccattgcggc aggccaatcg gtgtagtctg
     2701 accgctcgaa tcgagtacta gcgagcacga cgatgcgaac agcgcaatgg ccaccacaac
     2761 gatgatccag tgacacagct gcaccagcaa aggacgccac gaagcgggaa acagggcgcg
     2821 aaaactatcg atgccaatgt gctgcgcgcg tgccagcgtc gtcgctgccc ccagaaagac
     2881 ttgcatgccc atcagtgcac gtgcgatttc ttccgcccaa tccaccggat catgcaaaaa
     2941 atagcgccat acaaccgaac acagcaccac cagaacatcg ctggcaagca cgaaggccgc
     3001 catgatttcc gttgtgacca gcagtcgatc caggaagcgg tgtacgccgc ggacatgggt
     3061 aaactgggaa gcataagcgc ccggctgttt catgcttcgc gtatggctgc gctgttatcg
     3121 cgccgcggcg atcgccttga acaacggttt tgtctcaggg tactgctcgg caaatgcggg
     3181 ccagagtcgc gtttccatct ccgtgcgcaa cgctgaacgt tcatcttttg ccatggcatg
     3241 gaagctcaag ccgagcttcg caagtttgtc gaacgccgat cgtccttttt ccccagccac
     3301 ggcgcgttgg tacaggctgg cttcctgcgc agcccgcaga aatgccggcc ggatgtccgc
     3361 cgggatctta tcgaatcccc gcttgccgat gactgcaatc attggcggga acaagtgctc
     3421 ggtcagaaag caatgccttg tcacctcata gagtttggtc gccaacacgg tggaagcatc
     3481 atgttcgaaa ccctcgacca cgccagtctg cacggcggta tagagttcgt tgaacgggat
     3541 gggcgttggc accgcgccca tgagtttgaa ggtatcgatg aaggcacttg tcggcaagac
     3601 gcgcagcttt acgcccttta gcgcagctct cgacgtgatc ggtgctttgg tatagacact
     3661 gcgcgccgca aaatgggacc cccatccgag cacgatgcaa gcggtgcgct tttgcagcag
     3721 ctcctcgagg ttgccaccga cgccgccgtc cagcgcccta gccatatgtg cgtaactgtc
     3781 aaagatgtag cctaggtcaa gcatgccgaa ttccggtgct accgtggccc agatggacga
     3841 acctgtcagc atgatgtcga tggagccgac tttgacttgt tgtacaacgt cggcttcctt
     3901 gccgagctgg ctgtttggaa aaaaatccgg cgcgatgcgc gaacctgtgc cagccctcaa
     3961 attgtcagcg aagcgttgga accagatgta atgcgcggaa ttctcgtccg ctgtcaaaga
     4021 tgaggagata cgcaatggca ctgatgcttg cgctttagca gcaattggaa tgccagccga
     4081 aaacagcgcg gctgatgcag taccggcggc caggaaacgg cgacgcgaac ccgatgtggt
     4141 cattattttc ccccccggaa tatgcttgcg atcaggcgcc caccgaggct ctgaccggag
     4201 aacgcctgct cctgaccagc agcatcagag cgattgccat gttcaccaga ttccacgcga
     4261 ttccattcaa gaacgccatt ctgtacgatc cggttacgtc gaaaattatg ccggacatcc
     4321 agccgcccaa tgccatgccg accatcgttg cggtaagtac gatgccgatg cgcgtaccag
     4381 cctccttcgg cgagaaatac tcccggatga tcatcgcgta ggaaggcacg atgccgccct
     4441 ggaatagccc gaacaaagcg gagatcacgt atagcgaagc cagtccgtcg aaaggcagga
     4501 acaggaacag tgcgaagcat tgcatggtcg atccgatcag taacgttcgc agcccgccaa
     4561 tgcggtcgga gaccatgccg aacacgaggc gactgacaat cccgcatccc agcagtagcg
     4621 acagcatttg cgctccccga gccgcaccgt aaccgaggtc cccacagtac gctaccaaat
     4681 ggacctgcgg catagacatc gcgacgcaac agcatacgcc ggcgacgata agcagagtct
     4741 gtagactttg aggcgataac cccaacgggc ggcttcgatt tgcatggtcg gaagtcgccg
     4801 gggtgacagg ctcatcggtg gacggtaccg gcttgcgtaa ggcaagcacc agcggaaaca
     4861 tggttgcggc acaaaagatc gcgatgccaa ggaatgtcgg tcgccagccg ttgctttcga
     4921 tgaagtgctg gacgattgga ggccatagtg tgcctgcaag atagttgcca ctggcgatga
     4981 tggacactgc gataccgcgg cgccgctcga accaatggga tgtatctgcc agcagtggcg
     5041 agaaggtcgc agaactgccg aaaccgatca gcaggccatg cgcaagcgcg tattgccaga
     5101 gtgtcgaagc cgaggccgca gcggcgtaac cgaagatcag acacacgatg ccgaccatca
     5161 ccggtggaac gatgccccgc cggtctgtca tgcgacccag aacaattccg ccagcgccga
     5221 atccaagcat ggtcagcgtg tacgccagcg aagcatcccc gcgcgcgacc ccgaattcgg
     5281 cctgtacggc tggcaatgcc acgatgacag accacattcc gacgccgccg atcgtggaca
     5341 gcaacaatgc cagtacaagg cgcgtccatg cgtatgaagt gtccactcca ccgcgcgcga
     5401 cctgcctgtt gtttcttttg ttttccaccg cgctggattg tgcctcaatc ttcaaacatg
     5461 tgattcacat tactccgcaa gctctccaac actacgttac aaatttgctt caaccgctga
     5521 tggattattt gcgttatcaa caatgtgatg cattcaagcc aggaaaggta cgggtaatct
     5581 tatgaagaaa ctgtcaactg ccattgcctg cgggctcatg ctggcgcaac tgaacgcttt
     5641 cgcgtttgag gccgtcgcca gggtagtcgc ggtatcgccg attatgcagc aggtaaatca
     5701 accgtcgcag caatgctgga cggaaacgca accggtgacg caggcggcgc cacagcagca
     5761 caattatgtt ggcgcaatcg tcggcggtgt tgccggcggc ttgctgggcg cccaggtggg
     5821 tcgcggcaac ggtcgagtcg ccggcgcagc ggtcggggcc ggagtcggcg ccgtggccgg
     5881 agacgctgtg gcaaatagct ccaatggcac ggttacgacc atgacgccag tgcagcgctg
     5941 ccggcaggtg gagaaggtcc agtccgtaac cacgggttat atggtgacct acgagtacga
     6001 cggacaacgc ttttctacgc ggctacccta taaccccggc aaccagcttc gggtaaatgt
     6061 ggccgtcacg ccgcaccagt aaggtccgtt cgaataccag acaacaagaa actggttttt
     6121 ggctcttccg ggcctgaacg gcgacttctg gtagcatcgc cgctcgcgaa ccggaggggg
     6181 aaacaatgaa gctcgaagga tcggtgtgca ttgtcaccgg ctcggccacc ggtaccggag
     6241 cggcctgcgc gattcacctg gccaagaaag gatgtcgcgt tgtcgtcaac tacaccaaaa
     6301 gcgaaaaaga ggcggtgcaa acgcttgact cctgcaaggc ggccggaacg gatgccatca
     6361 tggtcaaggc agacgttgcg gccgacatgg actgtcgcgc gctcgcgaaa gcggccctcg
     6421 acaagtgggg acgtatcgat gcactgatca ataacgcggg cattaccaag ttcgctgctg
     6481 cagcagatct cgatggcctg gatgctggcg acttccaacg gctctacgcg gtgaacgtga
     6541 ttgggcccta ccagatgatt cgcgcttgcg cgcccgtaat gaagcgccag ggtaaaggcg
     6601 cggtcgtgaa catctcttcc atatcgggcg tcatgggaat aggttcatct actgcctacg
     6661 ttgcgtccaa gggcgcactc aatgcaatga ctttggcact cgcgcgttcg ctggcgcccg
     6721 aaatacgcgt taacgcggtt tgtccgggat tgattgaaac acgttggcat ctatcgcgct
     6781 tcagcaagga agactatgct aaattcaagg ccggttatga aaaaacggtg ccgcttgcaa
     6841 aggccgccag ccctgaggat gtcgccgaag tcgctgtctg gctgatcgaa ggggcggatc
     6901 ttgtgacggg agaatccata ctcgtggacg ccggactcca cttgggcaag ttctaagccg
     6961 aaaccagaag cagccaacgc aactatcacc aaggtgatag ctactgactc gatcggggaa
     7021 ttgcgatatt ccggaccttt ggccaatatg aactctggaa gcatgcctgc ttccggggcc
     7081 atcgagatac agcaagttgg ccgcgtttag cacctgccgg caggcaacgg cagaaaatga
     7141 caaggagatt tgcatgcgta tattgattcc cgcgctggca gcagccagca tcctgatgat
     7201 ctcgctgcca agctattcgg cagacgatgt gaaatcaggt ggcacttcca ccaagcccgc
     7261 tcgtgctctc gatgatggcg cggtgaagtc cggtggcgac accaacaagc ccggccgtgc
     7321 tgttgaagac aattcggtca aggcagctgg cgccacgagc aagccaggcc gtgcagttga
     7381 agaaggcacc gtaaagtccg gtggtgatac caccaaaccg gctcgcgctg tcgaaggcga
     7441 caagaaggcc gccaagaaga agaaaaagaa gaaagtagaa acgcagtaaa acagtactgc
     7501 atccttcgcg atcagtacga aaagaaggca ggttaccctg ccttcttttt tttgtggctg
     7561 gatatccgaa gctagcaatt agcttataac cccatccgca tttttgagcg ggatttattt
     7621 ttttaaccag ccaaggcgct gcgcctgctt gaccgcttga gtgcggttat ccgcctccag
     7681 acgccggaag agatctttca aatgctgttt tatcgtgcct tccgtgacgc ccagcacatc
     7741 ggcaatctgc ttgttcggca tgccttctgc cagtagcggc agaatttgct gttgccgcga
     7801 cgtcagcgcc gacatctggc cgcctgccgc agacccggaa gccggaatga acagcgcctc
     7861 cggcaaataa acctcgcctt gcaaaatgcg acgcagtgca tccatcagtt gttcactttt
     7921 ggctaacttc ggaatgtagc cccgcgcgcc gccgtctatc aactgtcgaa tgatccgtgc
     7981 gtcgttgaca ccggaaacga tggcgaccgg caaattcggg taacgccgtc gaatggtgta
     8041 aaagccttcc agcccattca tgcccggcat ttccaaatca agcaacagca gatcgacagg
     8101 ccactcgagc gcaagcttgt ccagcgctga ttgaagattg cttgcctcga gggccacggc
     8161 ttgcgcatgc aacagcttga gaaaaaatcc aagaccttcg cgaaacaggg aatgatcatc
     8221 agcatgcagg attttcatgt gtgcgtcagc gcatcgggtg gtaacttcaa tcgtgcgttt
     8281 tccaactgcg atttccctgg acttctaatt acggtgcggc cggcagttca aagaaaaatt
     8341 cgttgccgag ttgcgggccc ggtcggacgc cgacatttcc ccccagtctg gacaagatgc
     8401 gttgaacggt ggcaagtccg aggccgtggc cgggtgcgcg ctcctggtgc aggcgcgtga
     8461 agggaatgaa tacgcgcctg cgttcgtgtt ccgaaagcgt ctgcccattg tcccggaccc
     8521 agaagcgcac ttgcgaccct tccgacgcac cacccagttc aacctgtgcc ggcgagcctc
     8581 cgtatttcag cgcattgctt accagattca gccacacttc accgacccat ggctcatagc
     8641 ccaacgctga aggccaagtg tccggtaatt tgacttgagc tcccgtacgt gactgcagat
     8701 cggcgagttg gctcagtacc tggacgacca cgctgttcat gtccacggga tgcatttcga
     8761 cgttctgttg cgcaacggtg gaaagcaaca gcaacgcatc aaccgcctcg cccgtcattg
     8821 cgagcgcact ggaggacttt tgcaacaaat ctcgctgctc ggccgccaag ccgtctgact
     8881 tgaatttctg gagaatctcc aggaatcggc aagcggccgc aagcggtgtc ttgagactat
     8941 gcgcaatcgt atgtgcatag gcctccaatt ccgcgttctt ctgcgcgaga tcatcgctct
     9001 tggcacgtag ttctttctgt agagtatgta attgcaaatg gtgctgaatc ctggccaaca
     9061 gctcttcacg ctggatcggc ttggtgatgt aatcgacggc acccaacctg aagccttgca
     9121 ccttgtgcgc agtatcgtcc aatgccgtca tgaaaatgac cggcacactg gcagtgcgcg
     9181 gctcaatctt gagacgccgg catacttcga agccgtccat ctccggcatg gttacatcga
     9241 gcagtacgag gtctggcatc gaccgttgga ttttttccag tgcctgcgcg ccgctccgag
     9301 tgagcatcag ttcgaacccc tgttccgcga gaaagtccga tatcagccgg agattctgcg
     9361 gcttgtcgtc gacgatcaac aacgtcgcct cccggcgctc agccggcaac gtcatcacgg
     9421 cttgtttcaa ggcaatcctc cagcatcgcg accagccgct cttcctcgaa gccccgtgcc
     9481 agcgtgtaca agcgctgcga gaacgacgcg taacgagcgt cctggttttt tagatccgat
     9541 gccatttgct ccacacgcac caacttaccc atgcgcgcta aatcgagaag ttgctccaat
     9601 gcggcgcggg gcggcaaaac ggtttcgccg ttgtcctctt tcgctggacc agcggcgggc
     9661 agttccgctt ccagataacg ccagttcaac gcaagcgagg cctggatggc atccagcaac
     9721 tcgctcgttc gaaaaggctt gcgcaagcac tcgatgaaag taaccggatc ggcttccgca
     9781 cggtcgaggt ctgcgctgct ggccgaggca gcgacaatgg caatatgctc cagccctggc
     9841 gtgttgcgga tcgcacgtgc ggcttccatg ccatccatcc cgggcatgcg caaatccatc
     9901 acgatcagat caggacgatg tgcgcgagca cgcgtcagag cctcctggcc gccatcagcc
     9961 agcatcacct cgaatccaag cggttcgagc agctgcttta acaggcgacg gttttcttcc
    10021 tggtcgtcca cgaccaggat ggaccgccgc acgccactgt agccaacgat gcgttcgtca
    10081 agtgaagcta catcgtgctg cgtttgagcg atgggaagct ttaccgtgaa acggaactga
    10141 ctgccgcgcc ccaactggct gtcgactcca acatctccac ccatcaggcg cgccagttca
    10201 cgggtaatgg caagtccgag cccgacgccg gactcctgct tgtggccctc ttctgcctgt
    10261 tcaaatggcg caaaaatgcg cgacgtatct ttcggcgaaa tgccggcacc ggtgtccctc
    10321 acggatacgc gcagttcaac actaccaatt tcctttggta cacactgcac gcttaaagtc
    10381 acccctccgg aatcggtgaa tttgatggcg ttgcctaaca gattaagcag gatctggcgc
    10441 aagcgcttgc cgtctgcctc taccgtgtcc ggaagtccag ccgcgatttc cagaaaaaaa
    10501 tccaggcctt tccggtgcgc ttctacacgg accattgcgg cgatttcttc gaacagggcg
    10561 gccagttgaa ccggagccgg attcagttcc aggtgcccgg agcgaatgcg agacagatca
    10621 agcaggtcgt tgatcagaga aagcagatgc cggccactgc tggcaatcgt agcgagggct
    10681 tcccgccgcg actggtccgg catcgtgccg ccaccggcga gatcggcgta gccgagaatg
    10741 gcatgcagcg gtgtgcgcag ttcatggctc atgttggcaa ggaactggtc tttggagcga
    10801 ttcgcagcct ccgcttcagc gcgggcagcc agcagggcct tgcgctg
//
LOCUS       DATMZQ010000433         5742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1558525, whole genome shotgun sequence.
ACCESSION   DATMZQ010000433 DATMZQ010000000
VERSION     DATMZQ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558525"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /gene="otsB"
                     /locus_tag="VN496_13850"
     CDS             <1..632
                     /gene="otsB"
                     /locus_tag="VN496_13850"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HXF18088.1"
                     /translation="LLERAHRLNGRAVALISGRNLSDLDRLFGPMHFPAAGQHGLERR
                     DSMGRITRATDRLNRISRAACALEDQARVREGILVEHKGLSLALHYRSAPELREWAEF
                     TMRELLNRLGPEFQLIAGKMVYEVKPGGRDKGTAIADFMLEPPFVGRVPVFIGDDSTD
                     EDGFTVVNLVGGHSIKVGHGTSLAQWHLCDAHSVRRWLAAYTEFLNEGN"
     gene            635..2422
                     /locus_tag="VN496_13855"
     CDS             635..2422
                     /locus_tag="VN496_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXF18089.1"
                     /translation="MSDLNLALIGNCNIGALINAQAEIVWGCFPRFDSDALFCSLLQG
                     KHEPDEIGLWGIDILDHARTEQQYVPDTPILVTTLHDKQGGAVELTDFAPRFQQHGRI
                     FCPMMLVRRVKRIAGSPRLRLRMRPASNWGAQRASVTYGSNHIRYITSGVVLRLTTDA
                     SITALLQETPFFLDEEITLLLGPDETVPAGVREVGRRFEDETLNYWNDWVRNLAVPFE
                     WQEAVIRAAISLKLNAFEDTGAIIAAMTTSIPEALDSGRNWDYRYCWLRDAYFVVNAL
                     NRLSTTQTMERYLGYIINVAAGAPNGRLQPVYRIDGHPALHEETVTTLSGYRGMGPVR
                     LGNLAYLQVQNDVYGSAILAATHVFFDRRMNRPGNADLFRRLERLGDIAQHVFSEPDA
                     GLWELRGTSRVHTFSSVMCWVACDRLARIAAQIGLADRARHWRAHADRMHREICTRAW
                     NEKRGILGASFGGDGLDASLLLLPELHFISADDPRFRATMAQIEKELKQGDFVFRYGE
                     EDDFGVPQNAFLVCTFWYIDALAATGRKDEARELFKTMMARRNPHGLLSEHIDPKTGE
                     QWGNFVQTYSMVGLINSAVKLSLPWEEAF"
     gene            2532..3584
                     /locus_tag="VN496_13860"
     CDS             2532..3584
                     /locus_tag="VN496_13860"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF18090.1"
                     /translation="MPESKLEQAVQFAIDHETPWSREIGKKWGIHHEDPAPWNRLFGP
                     VHARGPVSGTMLLEGKTAISWGEPARADLTFSIAKTYLALIAGIAYDRGLLPDVDEPV
                     RARVAGAGFDEGRNAQVTWAHLLQQTSEWQGACFDIPDQVDRYRVLQFQGGTPQGKKG
                     DPRPLQKPGSFWEYNDVRINQLSLALLHLFRRPLPEVFHEAITQPIGASDAWRWVGYD
                     NSWIELDGKRVQSVPGGTHWGGGVSIGSLDQARIGQLLLDDGMANGERIVSAEWIQRM
                     RAPCEIAPFYGYLVWLNHARRIFPSVPASSYFAIGAGASFTWIEPQRRMVLVVRWINA
                     DHADEFFGRILQSLDA"
     gene            3696..4616
                     /locus_tag="VN496_13865"
     CDS             3696..4616
                     /locus_tag="VN496_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HXF18091.1"
                     /translation="MGSRVPLIEPVVTLDLDNWRPQIAPELSEHLAMSLEKGKVVCLP
                     NMRFQVSAAEQKFLSPVWLSGKRKNISLEGDSVKGANGSPEAMSALAAMIRRYSEQAT
                     DLVTRLFPKYREYLKQARTSFRPGVVNDTPSSWRKDDSRLHVDAFPSRPNHGRRILRV
                     FTNVNNQGVARVWRVGEPFETAARHFMPTIPGQLPGSARLLHALRITKAARSRYDHIM
                     LHLHDGMKADLDYQKNSPQQEVHFEPGTTWVCFSDQVLHAAMSGQYMFEQTIHLPIAA
                     QYHPELSPLSTLEKITNRMLVERDSLAEVR"
     gene            4700..>5742
                     /locus_tag="VN496_13870"
     CDS             4700..>5742
                     /locus_tag="VN496_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXF18092.1"
                     /translation="MQPWDISLFSIFHVLLAGTVSTHIVLTKPDVRAAIGWAGLVWLA
                     PVVGSALYALFGINRIRREAGRMRRGRVFFWIQEPRPFALLPYEVTLPQAVPQESRPL
                     ATLVGAITKSPLVPGNAVDTLVNGDEAYPAMLDAIDNAQSSVALATYIFDRGLVADKF
                     IDALERAMRRGVAVRVLVDGVGARYSHPQIVPELRKHGITAARFLAPAIPWRHPYFNL
                     RNHRKLMVVDGAVGFCGGMNIRDRCLLALKTKDKEQDIHFRIRGPVVSQLMTALAFDW
                     HFVTKEQLAGPAWFPVVPPVGSVLARGIADGPDEDFEALPLTMLGALSQATRSIRIVT
                     PYFLPDPTLIDALR"
BASE COUNT         1147 a         1674 c         1744 g         1177 t
ORIGIN      
        1 ccctgctcga acgggcccat cggttgaacg gccgcgccgt ggcgttgatc agcggacgca
       61 atctgtccga tctggaccgg ctgttcggac cgatgcattt cccggctgcg ggccagcacg
      121 gcctggaacg ccgggacagc atgggacgca tcacacgtgc caccgaccgg ctcaatcgca
      181 tcagccgcgc agcatgcgca ctcgaagatc aggcacgcgt gcgcgaaggg attctggtcg
      241 aacataaggg actcagcctg gcgctgcact atcggagcgc tcccgaattg cgtgagtggg
      301 cggaattcac catgcgtgaa ttgcttaacc gtctcggccc cgagttccag ctgatcgccg
      361 gcaagatggt ttatgaagtg aagcccggcg ggcgcgacaa gggaacggcc attgccgatt
      421 tcatgctgga gccgcctttc gtcgggcgcg tgccagtctt catcggcgac gattcgaccg
      481 atgaagatgg cttcaccgtg gtcaacctcg tcggcggcca ttccatcaaa gtaggccacg
      541 gtacttcgct tgcacagtgg catctttgcg atgcgcacag cgtgcggcgt tggctggcgg
      601 cctatacaga gtttttgaat gagggaaact gatcgtgagc gacctgaact tggcattgat
      661 cggcaattgc aacattggcg cactgatcaa tgcccaggcc gaaatcgtct ggggttgttt
      721 cccgcgcttc gatagcgacg ctttgttctg cagcttgctg cagggcaaac atgaaccaga
      781 cgaaataggc ctctggggca tcgacatcct ggaccacgcg cgtactgaac agcagtatgt
      841 gcccgatacg ccaatactgg ttacgacgtt gcacgacaag caaggcggcg ctgtcgaact
      901 gacggatttt gcaccgcgct tccagcagca cggccgcata ttctgtccga tgatgctggt
      961 gcggcgagta aaacgcatcg ccggcagccc acggctgcga ttgcgtatgc gtcctgcgtc
     1021 gaactggggc gcgcagcgcg catccgtgac ttatggcagc aatcatatcc gctacatcac
     1081 gtccggtgtg gtgctgcgcc tgacgacgga tgcttcgatt actgcgctgc tgcaggagac
     1141 tccatttttt ctggatgagg aaatcacgct gctgcttggt cctgacgaaa ccgtgccggc
     1201 cggcgtgcgt gaagtcggcc ggcgcttcga agatgaaacc ctcaactact ggaatgattg
     1261 ggtacgcaat ctggccgtgc catttgaatg gcaggaagcc gtgattcgag ccgcgatcag
     1321 cctgaaactg aatgcgttcg aggataccgg cgccatcatc gccgccatga cgacttcgat
     1381 tccggaagcg ctcgacagcg gccgcaactg ggactatcgc tattgctggc tgcgggacgc
     1441 gtatttcgtc gtcaatgcgc tgaaccgcct gagcacgaca cagaccatgg aacgctatct
     1501 cggctacatc atcaatgtgg ccgcaggcgc gccaaacggc cgcctgcagc ccgtgtaccg
     1561 gatcgatggc catcctgccc tgcatgagga gaccgtgacg acgctcagcg gctatcgcgg
     1621 catgggaccg gtgcgcctgg gcaatctggc ttacctgcaa gtccagaatg acgtgtacgg
     1681 ctcggccatt cttgcggcaa cgcacgtgtt cttcgatcgg cgcatgaacc gccccggcaa
     1741 cgccgatctg ttccggcgac tcgaacgtct gggagacatt gcgcagcatg tcttcagcga
     1801 gcccgacgcc ggcttgtggg agttgcgcgg gacgtcgcgg gtgcatacgt tttccagcgt
     1861 catgtgctgg gtggcctgcg accgcctggc gcgcatcgcc gctcagatcg ggttggcgga
     1921 tcgcgcccgt cactggcgcg cacatgcgga tcgcatgcat cgcgagatct gcacgcgcgc
     1981 ctggaacgag aagcgcggca ttctgggtgc atcattcggc ggcgacggtc tggacgccag
     2041 cttgctgctg ctgccggaat tgcacttcat ctcagccgat gatccacggt tccgggcgac
     2101 gatggcgcaa attgaaaagg aactgaagca aggtgacttc gtctttcgct atggtgagga
     2161 ggacgatttc ggtgtgccgc agaacgcttt tctggtgtgc actttttggt atatcgatgc
     2221 gcttgcggcg accgggcgca aggacgaggc acgcgagttg ttcaagacca tgatggcgcg
     2281 ccgcaatccg catggactgc tgtccgaaca catcgatccc aaaaccggcg agcaatgggg
     2341 caatttcgta cagacctaca gcatggtcgg actgatcaac tcggcagtga aactcagctt
     2401 gccctgggaa gaggcgtttt agccggatat tattggcggc ggcaataaag ttgctggttg
     2461 cgcgctacgg tgcaacaacg tcagaatcgg ctgataatcg tctccaatcg atcggccatc
     2521 atcgtccctt catgcccgaa agcaaactag aacaggctgt gcaattcgcg atcgatcatg
     2581 aaacgccatg gagccgcgag atcgggaaga aatggggcat tcatcatgag gaccccgcgc
     2641 cgtggaaccg cttgtttggt cccgtgcatg cgcgcggccc ggtatccggc acgatgctgc
     2701 tggagggcaa aaccgcaatt tcctggggag aaccggctcg cgccgatctg acgttcagca
     2761 tcgccaaaac ctatctcgcc cttatcgcag gaattgcata cgatcgcggg ctgttgccgg
     2821 atgtcgacga accagtccgg gcgcgcgtcg cgggagcggg attcgatgag ggccgcaacg
     2881 cccaggtgac atgggctcat ctgctgcaac aaaccagcga atggcagggt gcatgcttcg
     2941 atattccgga tcaggtcgat cggtaccggg tactgcagtt tcaaggcggc acgccgcagg
     3001 ggaaaaaagg agacccgcgg ccgctgcaga agccgggctc attctgggaa tacaacgacg
     3061 tgcgcatcaa tcagttgtcg ctcgcgttgc tgcacctgtt ccggcgtccg ctgccggaag
     3121 tgttccacga ggcgatcacg cagcccatcg gcgccagcga tgcatggcgc tgggtggggt
     3181 acgataattc gtggatcgaa ctcgatggca agcgcgtgca atcggttccc ggtggcacgc
     3241 actggggcgg cggcgtctcc atcggcagcc tggatcaggc gcgcattggg cagttgctgc
     3301 tcgatgatgg aatggccaac ggtgaacgga ttgtttcggc agagtggatc cagcgcatgc
     3361 gcgcgccgtg cgaaatcgca ccgttctacg ggtatctggt ctggctcaat cacgcgcggc
     3421 gcatatttcc aagcgtgccg gcatcgagct actttgcaat tggcgccggc gcctccttca
     3481 cctggataga accgcagcgc cgcatggtgc tggtggtgcg ctggatcaat gcggatcacg
     3541 ccgatgaatt cttcggccgc atcttgcagt cgctggatgc ttgagaacta ttcggcacca
     3601 tgcccgtttg ccaggcagct gtcatcgtct ttgaatgaaa tagaatacgt tacctcgcgg
     3661 ctgtacaaag ccggttgaaa cgacaggagc attccatggg atcgcgtgta ccgttgatcg
     3721 agccggtggt gactctcgac ctggataact ggcgcccgca aatagcgccg gaactcagcg
     3781 agcatttggc gatgtctctg gagaaaggca aagtagtatg cctgccgaat atgcgatttc
     3841 aggtgtcggc ggcagagcag aaatttttgt ctccggtctg gctcagcggc aaacgcaaga
     3901 acatcagcct ggaaggcgat agcgtaaagg gcgcaaacgg ttcccccgaa gcaatgtcgg
     3961 ccctggcggc catgatccgg cgttactcgg aacaggccac cgatctggtc acgcggctgt
     4021 ttccgaagta ccgcgaatat ctgaagcagg cgaggaccag ctttcggcct ggcgtcgtga
     4081 acgacacacc gtcttcatgg cgcaaggatg attcgcgctt gcacgtcgac gcgtttccgt
     4141 cccgcccgaa ccatggcaga agaattctgc gcgtattcac gaacgtcaac aatcagggcg
     4201 tggcgcgcgt gtggcgcgtg ggcgaaccat tcgaaacggc cgcgcggcat ttcatgccga
     4261 ccattcccgg ccaattgcca gggtcagcaa ggttgcttca tgcattaaga atcaccaaag
     4321 ctgcacgcag caggtacgat cacatcatgc tgcatttgca tgacggcatg aaagcggatc
     4381 tggattacca gaagaattcg ccgcaacagg aagtgcattt tgaaccgggg accacctggg
     4441 tctgtttctc cgaccaggtc ctgcatgccg caatgtcggg acaatacatg ttcgagcaga
     4501 ctatccattt gccgattgcg gcccagtatc atccggaatt gtctccgctc agtaccctgg
     4561 aaaaaatcac gaaccgcatg ctcgttgagc gcgattcact ggccgaagtc cgctagcaaa
     4621 agatcagacc tgtctctcag cacctgccgt ctcccactga tccatccgaa acgacaatag
     4681 ctgcggcgga atgatcaaca tgcaaccctg ggacatcagc cttttcagca ttttccatgt
     4741 gctgctcgcg gggacggtat cgacccacat cgtgctgacc aagccggatg tacgcgccgc
     4801 cattggctgg gcaggtctgg tgtggcttgc accggtcgtc ggctctgccc tctacgcatt
     4861 attcggcata aaccggattc gtcgtgaagc gggtcgcatg cgccgtggac gcgttttctt
     4921 ctggatacag gagccgcgac ctttcgcgct cctgccctac gaagtcactt tgccgcaggc
     4981 cgttccgcaa gaatcccggc cgcttgccac cctggtcgga gccattacca agtcgccgct
     5041 ggtcccgggc aacgcggttg ataccctggt caatggtgac gaggcgtatc cggccatgct
     5101 cgatgcaatc gacaacgcgc agtccagcgt cgcactggcg acctatattt tcgaccgcgg
     5161 tctggtcgcc gacaaattca tcgatgcatt ggaacgcgcg atgcggcgag gcgttgccgt
     5221 gcgagtgtta gtcgatggcg ttggtgcccg ctacagccat ccacagattg ttccggaact
     5281 gcgcaagcat ggcattacgg cagcgcgctt tctcgctccc gccattccgt ggcgccatcc
     5341 gtatttcaat ttacgaaatc atcgcaagct tatggttgtg gatggggccg ttggcttttg
     5401 cgggggaatg aatattcgcg atcgctgcct gctggccctg aagaccaagg ataaggagca
     5461 ggacattcat ttccggatca ggggccctgt ggtaagccag ttgatgaccg cgctggcgtt
     5521 cgactggcac ttcgtcacca aggagcaact cgccgggccg gcatggtttc cggttgtgcc
     5581 gccggtcggt tctgtgctcg cgcgcggcat cgccgatggc cccgatgaag atttcgaagc
     5641 gttgccgcta accatgctgg gcgccctgtc ccaggcaacg cgctcgattc gcatcgtgac
     5701 gccctacttc ctgcctgatc cgacgctgat cgatgcactg cg
//
LOCUS       DATMZQ010000434         9993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_873779, whole genome shotgun sequence.
ACCESSION   DATMZQ010000434 DATMZQ010000000
VERSION     DATMZQ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873779"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VN496_13875"
     CDS             complement(<1..635)
                     /locus_tag="VN496_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXF18093.1"
                     /translation="MELTKRLEKCYAAAVHDVLRAMGHAQCVLPHTIKPLDPAKKLAG
                     EIYTVSGYIDQTRDAHDTLVQWTGLLSRAPSGKVMVCQPNTHMVALMGELSAETLHHK
                     GVLGYVVDGGCRDTDFILQLGFPVFCSFNTPADIVGRWVPDRFGEPVTIGNVTVCTGD
                     WLLADRDGVVIIPGKIAEQVVAKTEEVLLTENKVRSAILGGMDPQQAYLKFG"
     gene            961..4887
                     /locus_tag="VN496_13880"
     CDS             961..4887
                     /locus_tag="VN496_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HXF18094.1"
                     /translation="MPAQFQDPESGSDTSQLTPFDSLLAQCRDLVCERLSLALAGMLD
                     KAEETLSALANETRDVPSKKLYAQTRDKALAQREPIEKQFRTRYLREFQQRSNRVKRI
                     GDSFAEIDLSSLELELIGKEDLDESLKFNAMAARLRQYCDEELIALDQRVGVLLGDAS
                     LQTEDNPFTPQAICDAYKHTCRQIDPNVDVRMVLLRLFDDHVADEIRAIYKAVNALLV
                     QNSILPVIRLSAKKEAGKPAPSSRPAADLAKTTSPATEAPKANAPVAEAAKPILPAEP
                     RAADGEQDLFSILKNLRAASMTAMPASAAGGAAGYGHGTGVVPGHGTGGPGHAVAAGG
                     AAGQSGSMPVGVNQTVLQGAELMGLLTRIQLGDATATPGNLLPMAHAGSGGMGMENVL
                     RELKNTSVGTGMNQLDMLTLDIMSLVFDQLFDDPKVPVGVKGLIGRLQIPMLKVAIAD
                     KTFFSRKTHPARQLLDTLGDISLRLPADFNTSSPLFGNMEIILQELIDSFESNMDVFD
                     VARKRVETLIAEEDQRVQKETQSAASRIEQNERLGLAKTVAQAEIKARVRTANVPIEV
                     LEFLIEQWVQVLLVVHVVDGEDSETWKTALETMDLLIWSVEPKQTVDERRELAVIVPE
                     VLQRISSGLRKVDIEDAVRKAFFADLMALHTGIIGKPDVEQKPPAQQVSAVTERAIAD
                     AGIPVGSAAVDSTAAALSQSPAPETVSEATTALPPLEQPASVPPAQMAATPAPHAEPV
                     VAIQTAETAPPSAPDAVPEVTKEAATPQPAAEVSFDTLAAADVKPDSPLAAPTGTLPD
                     LEFIPVPLIPAPSADVAAIPVAQSATMPGLDFAVPAATQAPEAAPESVSSATGKSAFV
                     PDLEFILAPVANAAPTGQPTPLSLDIDLTDLTEGAAASGIALPPLPELNLVAQAISAQ
                     PEPTEPSALFAAPATSSGTAAVENAKAFTSSAPKPIDDALLSPGGQTSKPATSSAAAP
                     GAQPAAKSPAAPVSPAAGPTGGPGVVLPSAAKPGFSPIAPANAAPSVKPMAATPANAA
                     PSAKPMTVPATAASGLKPMAAPANTAPGVKPMTVPLSQTPRAPTASPTVPARPAPAAA
                     PARPAVPQSPATAKPGAPPQRPQPMRPGATPAMKSAVAPAARPVSSAPSGAGTAARAG
                     ASAGRPPATPPSRDDSRLVDALDFTASITVQNPFGGGKVQVDDLDFTVKPAASAASTA
                     APKVEAVPAELPAKLNLGTWVGIRDKDDKTMRRSAKLSFISPLKTRYLFVDKQGKTTL
                     DCSRGELARRFKLGEIVIMEKVPDVPLFDRITEGLVGKLGGTKPQR"
     gene            5006..6643
                     /locus_tag="VN496_13885"
     CDS             5006..6643
                     /locus_tag="VN496_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18095.1"
                     /translation="MLRVFVCFAVVGTAALSAAAEEAAKDHDHHTGHALGTVDFAVSC
                     SAQAQTEFNHAVALLHHMTYPQARQAFERVATIDPMCAMAHWGIAMTLFQPLWPTRPQ
                     RDSLQHGWEEVLRAKALQPPTRREKLFVESAEAFFLDPASPDYWLRIRRWEQALAKIH
                     AASPDDSEGAVFYALALLATAPSNVLDRSHSDRAAAILLRVYKTNPDHPGAMHYLVHA
                     NDAPGRERESLDITRKYEALAPRNPHALHMPTHIYTRLGDWDGVIRGNLLAADAALEF
                     PAGDRGQFVWDEFPHAIEYLIYGYLQKGADAAAVAQLQRLWSTERLEPSFKTAFHLAS
                     TRARYALERRAWSEAISIAPRQPANLDWDRFAWPEAIGCFARGLAAAHVGKFNEVHDQ
                     MSRLRALEDATRAAGEELFASNIQALRLELSAWQAHSEGRQEASVALMIEASELEAST
                     PKHAVTPAPTFPADELLGDLLMEQEQAAAALTAYQRSLERYPRRFNSLLGAARAARTI
                     PDEALARNFYQQLLALAEGGMRYPAVLEANSYLGRKR"
     gene            6680..7477
                     /locus_tag="VN496_13890"
     CDS             6680..7477
                     /locus_tag="VN496_13890"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXF18096.1"
                     /translation="MNTLDGCIALVTGAGRGIGAAIALRYARAGASVAVADLDEATAQ
                     AIAEQVRALGRAADAFAVDVREPAQSQAMVARVAQRFGRLDVLVNNAGVIRVRSLLDT
                     TPEDWDFIQSVNARGLFFALQAGARQMLRQSPLGPGRPSGKVINIASIAGRGGRAMLG
                     AYAASKATAINITQTAAMEFAPHLTVNSICPGAVGTDMWKQIDREWSEHEQRPLGSVW
                     QERTRNIPMRRAETPDDITGMAVFLASAEADYITGQSYNVDGGLVTS"
     gene            7482..7952
                     /locus_tag="VN496_13895"
     CDS             7482..7952
                     /locus_tag="VN496_13895"
                     /inference="COORDINATES: protein motif:HMM:NF042776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6714 family protein"
                     /protein_id="HXF18097.1"
                     /translation="MTPPDMQALVALVREAFSSVILEDGIGLREAQGIDDYADEQTCA
                     AYRASDEKLDWSRIPIDELNACYSSLSFFDAKGMRFHLPAFLIAELSGEYHQDMSLQL
                     AYLNDYSIAQYGLLSPAQRAAVRAYLQFILRDPRYGFSHPEVRRALDEYWTDRI"
     gene            7979..8803
                     /locus_tag="VN496_13900"
     CDS             7979..8803
                     /locus_tag="VN496_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HXF18098.1"
                     /translation="MQHLSRRTFIRNAALLAIARTVAGTGLATSLNARSQTGKAGEDI
                     KALFFDVFGTLVDWRTGVTRESRTILEPLGYKLDWVAFADAWRAEYQPGMEEIRSGKL
                     PFSKLDVIHRGMLDRIRPRFGLQTLTESVLHELNLVWHRLDAWPDVPAALRRLRRHFL
                     LAPVSNGNISLMVDLARRNDFRWDAILGAEIAGDYKPKPHVYLAAADALGLAPANCMM
                     VAAHSGDLKAAGGAGLHTAHVVRSDEFGPNTGESAPKAPVDYAVRSLDELADAFSI"
     gene            8838..9689
                     /locus_tag="VN496_13905"
     CDS             8838..9689
                     /locus_tag="VN496_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF18099.1"
                     /translation="MSFVRLLALTSLAMIAFAGNSLLCRFALRQTSIDPASFTTVRLV
                     SGAITLWLVVRFKRRNDGAGGGTWRSAMALFAYAAGFSFAYETLTAATGALLLFGAVQ
                     ATMIGHAVWTGEKPGGRQLLGVALALAGLIGLLLPGISAPPLNGTLLMLCSGAAWGIY
                     SLRGKGTGDATTVTSGNFLRAAAIGIGLSVLTLKHASVDAAGIGYAVASGALASGIGY
                     AIWYTALPWLGSTTAATVQLSVPMIAALGGVVLLGEHVTLRLALASVAILGGIAAVTL
                     ARPARNR"
     gene            9769..>9993
                     /locus_tag="VN496_13910"
     CDS             9769..>9993
                     /locus_tag="VN496_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18100.1"
                     /translation="MAHNLHRTFLLIALLLAFAQESFAVDENNPGHSAALGREAVAVL
                     TDYLKIDTTNPPGNEIRAAQFFKALFDREGI"
BASE COUNT         2044 a         2978 c         3026 g         1945 t
ORIGIN      
        1 ccgaatttca aatacgcttg ctgagggtcc atgccgccca ggatcgcgct tcgcactttg
       61 ttctcggtca gcagaacttc ctctgttttg gctaccactt gttcggcaat tttgccggga
      121 atgatcacga cgccatcgcg gtcggcgagc agccagtctc cggtgcacac tgtgacgttg
      181 ccgatggtaa ccggctcacc aaagcggtcc ggcacccagc ggcccacgat atcagcgggc
      241 gtgttgaagc tgcagaagac cgggaagccc aattgcagaa taaaatccgt atcgcggcaa
      301 ccgccatcaa ccacatagcc gagcacccct ttatggtgca gcgtctccgc agatagttca
      361 cccatgagtg cgaccatgtg cgtattcggc tggcatacca ttaccttgcc ggatggcgcc
      421 cgggagagca gtcccgtcca ctggaccaat gtgtcatgcg cgtcgcgcgt ctgatcgata
      481 taaccgctca ccgtataaat ctcgcccgcg agtttctttg cgggatcaag cggcttgatg
      541 gtatgcggca acacgcattg ggcgtgcccc attgcgcgca aaacatcgtg tactgccgcg
      601 gcgtagcatt tttcgagtct tttcgtaagt tccatgtctg gtctttcctg agcggcatgc
      661 aatcttcagg ctactgtggc cggtgttaac cccagcatag gtgcgccgat tcagtaacgc
      721 aaggcgggcc gcaacctgac tcgtgaagcc ggttgcggca tgatgcggta gcatgtgttt
      781 cgcgctccgg atcgtgcctt tttcgcaatt tcatcaggca gattcgaggt ttttttattg
      841 ttgtcaaaag ggcacaatgc gtcattgtcg atccattttc agcgtaatca accggctgag
      901 ctgtcccgcg attgcgggat ctgcacaggg cgctgcgatg gacgatagcc attaaagatt
      961 atgcccgctc aatttcaaga tccggaatca ggttccgata cgtcccagct gaccccgttc
     1021 gacagccttc tggcgcagtg tcgtgacttg gtctgcgagc gcctgagcct cgcactggcg
     1081 ggcatgctcg acaaggccga agagactctt tcggctcttg caaatgaaac gcgggatgtg
     1141 ccttcaaaaa aactgtatgc gcaaaccagg gacaaggccc tcgcccaacg cgagccgatc
     1201 gaaaaacagt tccggacgcg ctacttacgg gaattccagc aacgcagcaa tcgcgtaaaa
     1261 aggataggcg acagttttgc ggaaatagat ctgtcatcgc tggagctgga gctgattggc
     1321 aaggaagacc tcgacgagtc gctcaaattc aatgcaatgg ccgccaggct gcggcagtac
     1381 tgcgacgagg aactgatcgc actcgatcaa cgggtaggcg tccttttggg cgatgccagt
     1441 ctgcagacgg aagacaatcc ctttacgccg caggcgattt gcgatgcgta caagcatacg
     1501 tgccgccaaa tcgatcccaa cgttgacgta cgcatggtgc tgctaaggtt gttcgatgac
     1561 catgttgccg atgagatccg agccatttac aaagccgtga atgccttgct cgtacagaat
     1621 tcgatcttgc ctgttatacg cctgtcggcg aagaaggaag ccggcaagcc agcgcccagt
     1681 tcacgacctg cagccgactt ggcgaaaacg acatcgccag ccaccgaggc gcctaaagca
     1741 aatgcaccgg tcgctgaagc ggccaagccc atcttgccgg ccgaaccaag agccgccgat
     1801 ggtgaacagg accttttttc aatactgaaa aacttgcgtg ccgctagcat gaccgcgatg
     1861 cctgcatccg ccgctggcgg ggcggccggc tatggccacg gcaccggcgt agttcccggc
     1921 cacggcacgg gcggccccgg ccatgctgtc gccgccggtg gagcagcagg ccaatcgggg
     1981 tcaatgccag taggcgtgaa ccagacggtg ctgcaaggtg cggaactgat gggcttactc
     2041 acgcgcatcc agcttggtga cgcgaccgca acgcccggaa accttctgcc aatggcgcat
     2101 gccggttctg gcggcatggg catggaaaat gtgctgcgcg aattgaaaaa caccagcgtg
     2161 ggcaccggca tgaatcagct cgacatgctc acgctggaca tcatgtccct ggtgttcgat
     2221 cagctgttcg atgatccgaa agttccggtt ggcgtaaagg gactcatagg ccggctgcag
     2281 attccgatgc tgaaagtcgc gattgccgac aagacattct tttccagaaa aacccatccg
     2341 gcacggcaac tgctcgatac gctgggcgac atttcgttgc gcctgcctgc ggacttcaac
     2401 acatcaagtc cgctgttcgg caatatggaa atcattctgc aggagctaat cgatagcttc
     2461 gaaagcaaca tggatgtctt cgatgtcgcg cgcaaacggg tcgagacgct tattgcggaa
     2521 gaagaccagc gggtacagaa agaaacacag tcagcggcca gccgcatcga gcaaaacgaa
     2581 aggcttggtc tcgccaaaac ggtagcgcaa gctgaaatca aggctcgtgt gcgcaccgcc
     2641 aacgtgccga tcgaggtact ggagtttctg atcgaacagt gggttcaggt attgctcgtc
     2701 gtgcatgttg ttgacggcga ggatagtgaa acctggaaga cagcgctgga aaccatggat
     2761 ctgctcatct ggagcgtgga gccgaagcaa acggttgacg agcggcgcga acttgcagtg
     2821 atagtacccg aagtactcca gcgtatctcg tccggacttc gcaaagttga tatcgaggac
     2881 gcggtgcgca aggcattctt tgcggacttg atggcgcttc acaccggaat aatcggcaag
     2941 cccgatgtgg aacaaaaacc gcccgcgcag caggtaagcg ctgtgacgga acgcgcgatc
     3001 gcggatgcgg gaatcccggt cggatccgca gctgtggatt ccacagcagc cgcactgtcg
     3061 caatctcctg cgccagaaac tgtatccgaa gcgacgacgg cattgccgcc gctcgagcaa
     3121 ccggcatccg tgccgccggc gcaaatggcg gcaacccccg cgccgcacgc ggagccggtt
     3181 gttgcgatac aaacggcgga aaccgcgccg ccgtctgcgc ccgatgctgt gccagaagtc
     3241 acgaaggaag cggcgacacc gcaaccggct gcggaagttt cgtttgatac acttgccgca
     3301 gcggacgtga agccggattc accgcttgct gcaccaacgg gaactttgcc ggatctggaa
     3361 tttattcccg tgcccctgat accggcgccg agcgcggacg ttgcggcaat tcccgttgcg
     3421 caatccgcta cgatgcctgg cctcgatttc gcggtcccgg cagccaccca ggcgccagag
     3481 gctgcgcccg aatctgtttc gtctgccacg ggtaaatcgg ctttcgtacc cgatctggaa
     3541 tttattttgg cgcccgttgc aaatgcggcg cccaccggtc agccgactcc attgagcctg
     3601 gacatagatc ttaccgatct aaccgagggt gcggctgcat ccgggattgc gctcccgccc
     3661 ttgccggaac tgaacctggt tgcacaagcg atctcagccc aacccgaacc aaccgagcca
     3721 tccgctttat ttgccgcccc ggccacatcg tctggcaccg ctgcagtaga gaacgcgaaa
     3781 gcgtttacat catccgcgcc aaaaccaata gatgacgctt tgcttagccc gggagggcaa
     3841 acgagcaagc cggcaacaag ttctgccgct gcgccgggtg cacagcctgc ggccaaatcg
     3901 ccagccgcac cggtgtcacc ggctgctgga cctaccggcg gcccgggcgt ggtgctgcct
     3961 tcagcggcaa aaccggggtt ttcaccgata gcgccggcta atgctgcacc gagtgtgaaa
     4021 ccgatggccg ccaccccggc taatgctgca ccgagcgcaa agccgatgac cgtcccggca
     4081 accgctgcat cgggcttgaa accgatggcc gccccggcta acacagcacc aggcgtgaaa
     4141 ccgatgaccg tgccgctctc tcaaacgcca cgcgctccca ctgcatctcc aacagttccc
     4201 gcgcggcctg cgcccgcggc agcgcccgcg aggccagctg ttccacaatc gccggccact
     4261 gccaagcccg gtgcgccgcc ccagcgtccg caaccgatgc gtccgggcgc tacgccggca
     4321 atgaaatccg cagtagcgcc agcggcgaga ccggtatcat cggcaccatc cggtgccgga
     4381 accgctgctc gcgcaggagc atccgcaggc cggccgcccg ctacgccgcc atcccgggac
     4441 gattccaggc tggtggacgc gctggacttc accgccagta tcaccgtcca gaatccgttc
     4501 ggaggcggca aggttcaggt cgatgatctc gacttcacgg tcaagccggc cgcgagcgct
     4561 gccagcacag ccgcccccaa agtggaagca gttcctgcgg aacttccggc caagctgaat
     4621 ctggggacgt gggtcggaat tcgcgacaag gatgacaaaa ccatgcgcag gtccgcgaaa
     4681 ttatccttca tcagcccgct caaaacccgc tacctgttcg tcgacaaaca gggaaaaacc
     4741 acgctcgact gttcgcgcgg agagcttgca cgacgattca agcttggcga gatcgtcatc
     4801 atggaaaagg tgccggatgt gccgctgttc gatcgcatca cggagggcct ggtcggcaag
     4861 ctgggcggca ccaaaccgca acgttgaggc cggcagatac cggttcagtc acaattcgag
     4921 attgaaaccg gtgcagatac tttttagctg tgccgcggag ttgtgcgaaa actgtaaatc
     4981 gtagcggggt gatgcagtgg gacgggtgct tcgtgtattt gtttgcttcg cggttgtcgg
     5041 cactgctgcc ttatccgcag ccgccgaaga agcggccaaa gatcacgatc atcacacagg
     5101 ccatgcgttg ggcacggttg atttcgctgt cagttgctcg gctcaggcgc agacggaatt
     5161 caaccatgct gtcgcgctgc tgcatcatat gacctatccg caagcgcgcc aggcgttcga
     5221 acgcgtcgca acgatagacc caatgtgcgc gatggcccac tggggcatcg cgatgacgct
     5281 gtttcagccg ctctggccga cgcgcccgca gcgcgacagc ttgcagcacg gctgggaaga
     5341 ggtgctgaga gccaaggcgc tgcagccgcc gacccggcgc gaaaaactgt tcgtggaatc
     5401 agccgaggcg ttttttctgg atccagcatc accggattac tggctgcgca tccgccgctg
     5461 ggaacaggcc ctggcaaaaa tccatgcggc ttcccccgat gactccgagg gcgccgtatt
     5521 ctacgcgttg gctcttctgg cgaccgcgcc ttccaacgta ctcgatcgca gccattcaga
     5581 ccgggccgcc gcaatcctgc tgcgcgttta taaaaccaat ccggaccacc cgggcgccat
     5641 gcactatctg gtacatgcca atgatgcgcc gggacgcgaa cgcgagtcgc tggacattac
     5701 gcgcaagtac gaagcgcttg cgccgcgcaa tccgcacgca ctgcatatgc caacgcatat
     5761 ctatacgcgc ctgggagatt gggacggcgt tatccggggc aacctgctgg ccgcggatgc
     5821 ggccctggaa tttccagcgg gggatcgcgg ccaatttgta tgggatgagt ttccgcacgc
     5881 aatcgaatat cttatttacg gttaccttca aaaaggtgcg gatgccgctg ccgtggcgca
     5941 gctgcagcgg ctgtggtcga cggaacgtct ggagccgagc ttcaagaccg cgtttcatct
     6001 ggcgtccacg cgggcgcgct atgcactgga gcggcgcgcg tggagcgagg ccatttcaat
     6061 tgctccacgg caacccgcga atctcgattg ggatcgcttt gcctggcccg aggccattgg
     6121 ctgctttgcc cgcggactgg ccgcggcgca tgtcgggaaa ttcaatgaag tgcacgacca
     6181 gatgtcgcgc cttcgtgcgc tggaggacgc aacgcgcgca gcgggcgaag agttgttcgc
     6241 cagcaatatc caggcgctgc gtctggagct aagcgcatgg caggcgcaca gcgaaggaag
     6301 gcaggaggcc agcgtagcgc tgatgatcga ggcatccgaa ctggaagctt caacgcccaa
     6361 gcatgccgtt actccggccc cgacatttcc cgccgatgaa ctgctgggag acctgctcat
     6421 ggagcaagag caggccgccg ccgcgctgac ggcgtaccag cgctcgctag aacgctatcc
     6481 gcggcgcttc aacagcctgc tgggcgctgc ccgggcggcg cgaaccatcc cggatgaagc
     6541 acttgcccgc aatttctatc agcagcttct tgcgttggcc gaaggcggaa tgcgctatcc
     6601 ggcggtgctg gaagcgaaca gctatcttgg ccggaagcgt tgaacacggc tatcgaattt
     6661 caataccagg gctcgcatca tgaacacact cgacggatgc atcgcgctgg tgaccggcgc
     6721 gggacgcgga atcggcgctg caatcgcgct gcgctatgcg cgggcaggcg cgagcgtcgc
     6781 cgtcgcggac ctggatgaag ccaccgcgca agcgatcgcg gaacaagttc gcgcgctggg
     6841 tcgcgccgcg gatgcttttg cggtggatgt gcgtgaaccg gcgcaaagcc aggccatggt
     6901 cgcgcgcgtg gcgcagcgct ttgggcggct cgatgtgctg gtcaacaacg cgggcgtgat
     6961 ccgtgtgcgc tcgctcctcg acaccacgcc ggaggattgg gatttcatcc agtcagtcaa
     7021 tgcgcgcggg ctatttttcg cgcttcaggc gggcgcgcgc cagatgcttc ggcaatcgcc
     7081 gcttggaccc gggcgcccga gcggcaaagt aatcaacatt gcgtcgatcg ccgggcgcgg
     7141 aggccgggcg atgctgggtg cctatgccgc cagcaaggcc actgcgatca atatcaccca
     7201 gactgccgca atggaatttg cgccacacct gacggtaaac agcatttgcc ccggggcggt
     7261 tggaaccgac atgtggaaac agattgaccg cgagtggagc gagcatgaac agcgcccgct
     7321 tggctcggtg tggcaggagc gaacaaggaa tatcccgatg aggcgcgcgg aaaccccgga
     7381 cgatatcacc gggatggcgg tattccttgc gagcgccgag gccgactaca tcacgggaca
     7441 gtcgtacaac gtggatggcg ggctggttac gagctgaaag catgactccg cctgatatgc
     7501 aggcgcttgt tgccctcgtc agagaggctt tttccagcgt gatcctggaa gacggcatcg
     7561 gactgcgcga ggcgcaaggc atagacgact acgcggatga gcagacctgc gccgcctatc
     7621 gcgcgagcga tgaaaagctg gattggagcc gcataccgat cgatgaattg aacgcctgct
     7681 atagcagcct cagtttcttc gatgcgaaag gcatgagatt tcacttgcct gcatttctga
     7741 tcgcggaact gagcggcgaa tatcatcagg atatgtcgct gcagctggct tatctgaatg
     7801 attactccat tgcgcaatac ggcctgctgt cacccgctca gcgcgctgct gtccgggcgt
     7861 acttgcagtt cattctgcgc gatccgcgtt atgggttttc gcatccggag gtcaggcgag
     7921 cactggacga gtactggacc gatcggattt aaatgggtga aaaaagggag gaaccacgat
     7981 gcaacatttg tcccgtcgca cattcattcg caatgccgcg ctgctcgcga tcgcgcgcac
     8041 cgtggctggg accggccttg caacatcgct gaacgcacgg tcgcaaacag gcaaagccgg
     8101 tgaagacatc aaagccctgt tcttcgacgt attcggaacc ctggtggact ggcgtacggg
     8161 cgtgacgcgc gagtcgcgca cgatcctgga gccgctcggc tataaactgg actgggttgc
     8221 attcgcggat gcctggcgcg cggagtatca gcccggaatg gaggaaatac gctcgggaaa
     8281 attgccgttt tcgaaactcg atgttatcca tcggggaatg ctggatcgca ttcggccccg
     8341 gtttggcttg caaaccctca cggaatcggt gctgcatgaa ttgaatcttg tttggcatcg
     8401 cctggatgcc tggccggacg tgccagccgc attgcggcgc ctgagaagac attttttgct
     8461 tgcgccggta tcgaatggca acatctcact gatggtcgat cttgcgcgcc ggaatgattt
     8521 tcgttgggat gcaatcctgg gtgccgaaat cgcgggcgac tacaagccaa agccgcacgt
     8581 ctatctggcg gcagcggatg cgctcggact ggcgccggca aactgcatga tggttgccgc
     8641 acatagcgga gacctgaagg cggcgggagg cgccggcctg catacagcac atgtagtgcg
     8701 ttccgatgaa ttcggaccca acaccgggga gagcgcaccc aaagcgccgg ttgattatgc
     8761 agtgcgttcg ctggatgagc ttgctgatgc gttttcgatt tgaccggcga taaccttccg
     8821 cggcgccgtt aaccgcaatg tcctttgtcc gcctgcttgc tctgacgtcg ctagccatga
     8881 ttgccttcgc gggcaactca ctgctgtgcc ggttcgcgct tcggcaaacc agcattgacc
     8941 ccgccagctt cacgaccgtg cgattggttt caggtgccat aacgctgtgg ctggtggtgc
     9001 ggttcaagcg ccgcaatgac ggtgccggtg ggggtacttg gcgatccgcc atggctttgt
     9061 ttgcgtacgc agccggattt tccttcgcct atgaaaccct gactgcggcg accggcgcgc
     9121 tgctgctttt cggggccgtt caggcgacga tgatcggaca tgccgtgtgg accggggaaa
     9181 agccgggcgg gcggcaactg ctcggcgttg cactcgcgct cgcaggcctt attggtcttt
     9241 tgctgcctgg catttccgcg ccgccattaa acggcacgtt gttgatgctc tgcagtggcg
     9301 cggcgtgggg tatttattct ttgcgcggca aggggaccgg cgatgccacc acggtcacat
     9361 ccggaaattt tctgcgtgca gccgccatcg ggatcggatt gagcgtattg acgctaaagc
     9421 atgcgtccgt cgatgcggct ggcattgggt acgccgttgc gtccggcgca ctggcgtccg
     9481 gaatcggtta tgcaatctgg tacaccgcgt taccgtggct tggaagtacc accgcggcaa
     9541 ccgtccagct cagtgttccg atgatcgccg cgctcggcgg cgtggtattg ctcggtgagc
     9601 acgtgacctt gcggctggcg ttggcctcgg ttgcgatttt gggtggaatt gcggcggtca
     9661 cgctggccag acccgcgcgc aatcggtaac ttagatccgc ttgctgtccg gatgaaaatg
     9721 ccggcaacaa taacggcgtg ctgacaaacc ccttcaggag caatttacat ggcacacaac
     9781 ctccaccgaa cattcctgct gatagcgctg ctgctcgcgt tcgcacagga gtcttttgct
     9841 gtggacgaga acaatccggg acattccgca gccttgggcc gggaagcagt tgccgtactg
     9901 accgactatc tgaaaatcga caccaccaat ccgccgggaa atgaaatcag ggctgcgcaa
     9961 ttcttcaagg cactctttga ccgcgaaggc ata
//
LOCUS       DATMZQ010000435         5878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_876229, whole genome shotgun sequence.
ACCESSION   DATMZQ010000435 DATMZQ010000000
VERSION     DATMZQ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876229"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VN496_13915"
     CDS             <1..525
                     /locus_tag="VN496_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HXF18101.1"
                     /translation="AGFSEDYPQDEQEVVRPEGDAWLRYGNNLLPIGFQPRDASSPVF
                     AYPYAKSRQSLLQLAQVDKPHASHGHKMEFVNPATGGPAMPTMGAYIQYLPRGFSGRP
                     YRSTDGTVFSVIEGAGRVVVEGETFAFSARDTFVVPSWQRYTLHAMEGCMLFSYSDRP
                     VQRALGIWREQQLQ"
     gene            588..1283
                     /locus_tag="VN496_13920"
     CDS             588..1283
                     /locus_tag="VN496_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXF18102.1"
                     /translation="MNASELLFEIGAVPSVPVVGRKERFPVHRIYCVGRNYAAHAREM
                     GKDPDKEPPFFFLKPADAVLPGGGKMHYPPGTQNLHHEIEMVVALRGEGRNIPAAKAL
                     ELVFGYAVGLDMTRRDLQFKARDMGRPWDFGKAFDESAPISAIHPVSSNGHHSTGKIW
                     LKVNDEMRQEADLSEMIWNVPDTIAFLSEYYLLKPGDLIFTGTPAGVGAVKKGDRMLG
                     GVEGLGELSVTVE"
     gene            1300..2190
                     /locus_tag="VN496_13925"
     CDS             1300..2190
                     /locus_tag="VN496_13925"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXF18103.1"
                     /translation="METPSPGDTWLEVALNGPWTRKHQPRIPVTIAEIVEDGIACVEA
                     GAAIVHAHAYDEATGQQTQNADVYARIIDGIRSKVDAIVYPTIPAAGLGSQHMQNAQQ
                     RFAHIEELARRGLIEWAAVDPGSTNFALYDDLLEDKSGFVYLNPEEDIRHGLKLARRF
                     GLHPSYAIYEPGFMRLGASLHWRESCPAPIYRLMFSSGYTFGFPPEDYALTAYLKLLD
                     QVAPGAKWMLAGLQVDVTPMIPRAIVEGGHVRVGLEDAPWGSDKINVRWVEEAALRIE
                     NAGGKLATAQEIRAALSPDE"
     gene            2361..3641
                     /locus_tag="VN496_13930"
     CDS             2361..3641
                     /locus_tag="VN496_13930"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HXF18104.1"
                     /translation="MRRATVILKSSLLRALLSLGFWSLLAADVGAAGNATYPRLMGMN
                     IGAKNYQDARYQQDLSRLDVVILGFYKGWQPAGYAPTPSAAMSKVVQEIKARHPGILV
                     GQYTVMNEVYGDPADVATADLRAKLDANKWWLLNASGRKVQWTDQYNAWETNFTGWAA
                     PDAEGRRWPEWLAERNYSVFFRDVPEFDIVYLDNIMGIARVRGSWKQDGHDARPDDED
                     VEQAHRAGHVAHWKRIRQLQPKALLIGNADNDLSNDQWRNQLDGAFLEGLMGEHWSIE
                     KRRGWGKMMERYRAALENTREPKIVGFNVSGAPDDYRFFRYAYTSCLLDDGYFSFTDK
                     SRGFSSVPWFDEYDYKLGHAQSAPPLEPWHEDIWRRDFENGVVLLNPTSEAKTVTLEP
                     GMRRLAGKQDAAVNDGSAVTRLTLRSKDGIVLRR"
     gene            3784..4764
                     /gene="pdxA"
                     /locus_tag="VN496_13935"
     CDS             3784..4764
                     /gene="pdxA"
                     /locus_tag="VN496_13935"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXF18105.1"
                     /translation="MTAKIGITMGDPSGIGPEIIVKTLAQMSPAVRSLSVVVGNLAIL
                     KRADKLLGTGLKFINGLDARNGEVPVADVATKDQDKIVDGKVSAAGGEAAYRYVEAAV
                     RLALDHKIEVIVTAPLNKAAMHSAGHHFDGHTELLAHLTGAKSSFMLLASDKLNAIHV
                     TTHQSLKNAAERPTIERVVATICAGYEHFRQLGIDHPRIAVAGLNPHSGEGGIFGREE
                     IERIQPAIEIARKQGIDVKGPISGDTVFYRALKGEFDLVVAQYHDQGHIPTKLIAFDE
                     TVNVSLGLPIRRTSVDHGTAHDIAWTGKANNVNMQSAIAYARKMADHIAA"
     gene            4868..>5878
                     /locus_tag="VN496_13940"
     CDS             4868..>5878
                     /locus_tag="VN496_13940"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HXF18106.1"
                     /translation="MAAQPRVLIVADDLTGAMDSAGPFAALGVETWVVAMPMRCDPAS
                     LKSARVISVNTETRHLPATQAAARVREIVRHLDCAGYDVVVKKIDSTLRGNVVAETLA
                     LMEASARREAIVAAAFPAQGRTVHNGRVHVDGKPLAETAFARDALSPPPLMPLQDVFA
                     AAKDGLRVKSAGPADAFDGATDIWIADSASDADLATIVKKFVSRTGRVLIVGSAGLTQ
                     ALASACFDEQPRPVRLHNISGTIVFAVGSRAARSAEQVEALAAEAHTGIVLAPNGRLL
                     SGKLPAAANIVLKATQGDAGNESNAECVAAELAKHAIEIASRTQAQALVATGGDTAIA
                     ILA"
BASE COUNT         1219 a         1662 c         1812 g         1185 t
ORIGIN      
        1 gccggtttct ccgaagacta tccgcaggac gaacaggagg tcgttcgtcc tgaaggcgat
       61 gcctggctgc ggtatggaaa caatctgctg cccatcggct ttcagccccg ggatgcgagt
      121 tcgccggtgt ttgcctaccc ctatgcgaag tcgcggcaaa gcctgctgca gttggcgcag
      181 gtcgataagc cacatgcaag ccatggccac aagatggagt tcgtcaatcc cgctaccggc
      241 ggcccggcca tgccgaccat gggcgcttat atacagtact tgccgagagg gttcagcggc
      301 cggccttacc gcagcaccga tggcaccgtg ttttcggtga tcgaaggcgc gggtcgggtg
      361 gtggtcgaag gcgaaacctt tgcattttcg gctcgcgaca cgtttgttgt gccatcgtgg
      421 cagcgttata ccctgcacgc catggaaggt tgcatgctat tcagctattc cgaccggccg
      481 gtgcagcgcg cattgggtat ctggcgggag caacaactgc agtgaccggc gacgctgccg
      541 gttctatcaa tgcgtttcca gacttaacct gttaccaata aaaaatcatg aatgcatccg
      601 aattgctgtt cgagattggc gccgtgccat cagtacccgt cgtgggacgc aaggagcgtt
      661 ttccggtcca ccgcatttac tgcgtcgggc gcaactatgc agcgcatgcg cgcgaaatgg
      721 gcaaggatcc ggacaaggaa ccgccatttt tctttctgaa gccagcggat gcggtgttgc
      781 cgggcggcgg caaaatgcac tacccgccgg gcacacagaa tctgcatcac gaaatcgaga
      841 tggtcgtcgc gctccgcggg gagggccgca atattccagc cgccaaggcg ctcgaactgg
      901 tattcggcta tgcggtgggg cttgatatga ctcgtcgcga cctgcaattc aaggcacgcg
      961 acatgggtcg cccctgggat tttggcaagg ctttcgatga atcggcgcct atctcggcca
     1021 tccatccggt ttcgagcaac ggtcatcatt cgaccggaaa gatctggttg aaggtcaacg
     1081 atgaaatgcg tcaggaagcg gacctgtccg agatgatctg gaatgttccg gacacgatcg
     1141 ctttcctgtc cgaatattac ttgctcaaac caggcgacct gatttttacc ggcacccctg
     1201 ccggtgtggg ggctgtgaaa aaaggcgacc gcatgctggg tggcgtcgag ggactgggtg
     1261 aattatccgt gaccgtcgag tagccggtcg acgatcagca tggaaacacc cagcccgggc
     1321 gatacgtggc tcgaagtcgc gctcaatgga ccgtggacgc gtaagcacca gccacggatt
     1381 ccggtcacca ttgccgaaat tgttgaagac ggtattgcct gcgtcgaagc cggagccgcc
     1441 attgttcatg cgcatgccta cgacgaagcg accggacagc agacgcagaa cgcggacgtt
     1501 tacgcgcgca tcatcgatgg cattcgcagc aaggttgacg ccattgtgta tccgaccatt
     1561 cccgcagctg gcctcggatc gcagcacatg cagaacgcgc agcagcgttt tgcacatatc
     1621 gaagaactgg cgcgccgggg cttgatcgaa tgggcggcgg ttgatcccgg gtcgaccaat
     1681 tttgcgctgt acgacgatct tctggaagac aaatccggat tcgtctattt gaaccccgag
     1741 gaagacattc gccacgggct caagctcgcg aggcgctttg gcttgcatcc aagttatgcc
     1801 atctacgagc ccgggttcat gcggctgggt gccagcctgc actggcgtga aagctgcccg
     1861 gccccaattt atcggctgat gttttcgagc ggctacacct ttggctttcc ccctgaagac
     1921 tacgcgctca cggcatacct gaaattgctg gatcaagtgg cgcccggcgc gaaatggatg
     1981 ctcgcggggt tacaggtgga cgttacgcca atgatcccgc gtgcgatcgt cgaaggcggg
     2041 catgtgcgtg tggggctgga agatgcgccc tggggctcgg acaagatcaa tgtgcgatgg
     2101 gtggaggagg ccgcgctgcg aattgaaaac gctggaggaa aactcgctac ggcgcaggaa
     2161 atccgcgccg ccctcagccc ggacgagtaa tgcacgcggg tgctccaagc agactatgcg
     2221 gtaagggttg caacatcatg actgtttttg atggcctgac catactgctt agagtggctg
     2281 ccggctagca gccaggctat tgaagccgcc atattccata acgcaaaaat agtttaggtc
     2341 gcgcaattcc ttggcaggaa atgcgtcgcg caaccgtgat attgaaatcg tctctgcttc
     2401 gtgcactgtt gtcgctcggt ttctggagcc tgctggcagc ggacgttggc gcagcgggca
     2461 acgcaactta tccgcgcctg atgggcatga atatcggtgc caagaactat caggatgcgc
     2521 gctatcagca agatctctcg cgtctggacg tggtgatcct cggcttctac aaaggctggc
     2581 agccagcagg ctatgcacct acgccgtccg cggcgatgag caaggtcgtg caggaaatca
     2641 aggcacgcca tcccggtatt ctggtcggtc aatacacggt aatgaacgag gtttacggcg
     2701 acccggccga tgtcgcgaca gccgatctgc gcgcgaagct ggacgccaac aaatggtggc
     2761 tgcttaacgc ttcggggcga aaagtgcagt ggaccgatca atataacgcg tgggagacca
     2821 acttcaccgg ctgggccgca cccgatgcag aaggccggcg ctggccagag tggttggccg
     2881 agcgcaacta ctcggtattt tttcgggacg tgccggaatt cgatatcgtg tacctcgaca
     2941 atatcatggg catcgcgcgg gtccgcggca gttggaaaca ggacggccac gatgcccgtc
     3001 cggacgatga agatgttgaa caagcgcatc gtgccgggca cgtcgcccat tggaaacgaa
     3061 tacgccagct tcagcctaaa gcgctgctga tcggcaatgc cgataacgat ctgtcgaacg
     3121 accagtggcg caatcagctc gatggtgctt ttctggaagg cctgatgggc gagcactggt
     3181 cgatcgaaaa gcgccgtgga tggggcaaga tgatggagcg ctatcgtgcc gcgttggaga
     3241 atacgcgcga accgaaaatc gtcggcttca acgtatcggg cgcccccgat gactatcgtt
     3301 tttttcgcta cgcgtatacc tcctgcctgc tggacgacgg ttacttttcc ttcacggaca
     3361 aatccagggg attcagcagc gtcccgtggt ttgatgagta cgactacaag ctcggccatg
     3421 cgcagtcggc gccgccgctt gaaccctggc atgaagacat ctggcgccgg gattttgaga
     3481 atggcgtcgt gctgctcaac cctaccagcg aggccaaaac ggtaacgctg gaaccgggga
     3541 tgcggcggct ggccggcaaa caggatgcag ccgttaacga cggttcggcg gtcacgcgcc
     3601 tgaccttgcg atcgaaggat ggcatcgtgc tgcgccgttg aaccgccgtt tccatctctg
     3661 cctttatcca tcatttcgcg ccgttgtggc gctgctgatc tttgcggctg cttgagctga
     3721 cgcagcggac taccatggtg cgtcggctat aatttggtcc attgtttcaa tcacgaggca
     3781 ggcatgacgg caaagattgg catcaccatg ggcgatcctt cgggaatcgg gccggaaatt
     3841 atcgtgaaga cgctcgcgca gatgagtcct gcagtgcgct cgctttccgt ggtcgttggc
     3901 aatctggcta ttttgaaacg cgccgacaaa ctgctgggca ccggcctgaa gttcatcaat
     3961 ggactggatg cgaggaacgg cgaagtgccg gtcgcggacg tcgctaccaa ggatcaggac
     4021 aaaatcgtag acggaaaggt ttcagctgca ggcggcgagg ctgcttatcg ttacgtcgaa
     4081 gcggcggtca ggcttgcgct ggaccacaag atcgaagtca tcgtgactgc accgctcaac
     4141 aaggcggcca tgcattcggc cggtcatcat ttcgacggtc acaccgagct gttggctcac
     4201 ctgacagggg cgaaatcttc tttcatgctg ctggcgagcg acaagctcaa tgcgatccat
     4261 gtgacaacgc accagtcgct caagaatgcc gccgaacgtc ccaccatcga aagagtggtt
     4321 gccacgattt gcgcaggata tgagcatttt cggcaactgg gcatcgacca tccccgcatc
     4381 gcggttgcag gcctgaaccc gcatagcggg gaaggcggca ttttcggccg cgaggaaatt
     4441 gagcgcatcc agcccgcaat cgaaatcgcg cgcaagcaag gcatcgatgt caaaggcccg
     4501 atttcggggg acaccgtttt ttaccgggca ctcaaaggcg agttcgatct ggtggtcgcg
     4561 caataccacg accagggtca cattccaacc aagctgattg cgttcgatga aaccgtaaac
     4621 gtcagcctcg gcctgccgat tcgccgcacg tcggtggacc atggcacggc gcacgatatc
     4681 gcctggaccg gcaaggcgaa taacgtgaat atgcagtcgg ccatcgctta cgcgcgcaaa
     4741 atggcagacc acattgcggc gtgaagccgg gatagcaccc gctcctggcg ttaccccttc
     4801 gacttattaa tcttcctgat aagctgcaag ccatggctat gcttcttgca tcgtctgcaa
     4861 aacctgaatg gcagcgcagc cccgggttct gattgtcgcc gatgacctga ccggcgcgat
     4921 ggacagtgcc gggccctttg cggcgctcgg tgtcgagacc tgggttgtgg cgatgccgat
     4981 gcgctgcgac cctgcttccc tgaagtccgc acgggttatc tccgttaata ccgagacaag
     5041 gcatcttcct gccacacagg cggccgctcg cgtgcgcgag attgtccggc atctggactg
     5101 cgccggttac gacgttgtgg tcaagaaaat agactcgacc ctgagaggca atgtcgtggc
     5161 cgaaacactg gcgctgatgg aagccagcgc ccggcgcgag gcgatcgtgg cagcggcatt
     5221 tccggcgcag ggacgcaccg tgcacaacgg ccgcgtgcat gttgacggca agccgcttgc
     5281 cgagacggcg ttcgcgcggg atgcgctgtc gccgccgccc ctgatgccgt tgcaggacgt
     5341 gttcgccgcc gccaaagacg ggctgcgggt gaaatccgct gggccggcag acgcattcga
     5401 tggagcaaca gatatctgga tagcggacag tgccagcgat gcggatcttg cgacgatcgt
     5461 aaagaagttt gtgtcgcgca cgggccgcgt gttgattgtt ggatctgctg gtctcacgca
     5521 ggctcttgca tcggcttgct tcgatgagca gcctcggccc gtccggctgc ataacatcag
     5581 cggcacgatc gtgtttgcgg tcggctcgcg ggccgcacgt agtgcggagc aagtcgaagc
     5641 actggccgca gaagcgcaca ccgggattgt gcttgcaccc aacggtcgtt tgctttcggg
     5701 gaaattgccc gcagcggcaa atattgtgct caaagccacg caaggcgatg cgggcaatga
     5761 gtccaatgcc gagtgtgtcg ccgccgaact ggcgaagcat gcaattgaaa tcgcaagccg
     5821 cacgcaagct caggcgttgg ttgcaaccgg tggcgacacc gccatcgcga ttctggcc
//
LOCUS       DATMZQ010000436         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_876403, whole genome shotgun sequence.
ACCESSION   DATMZQ010000436 DATMZQ010000000
VERSION     DATMZQ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876403"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VN496_13945"
     CDS             <1..335
                     /locus_tag="VN496_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HXF18107.1"
                     /translation="MPIQRFGSMLISKGQFNQYMHLLRDNFMAANLSNVMCRSLVSID
                     WQGYLYDCDFNQMLGLALPEQLVDGARPHLRDLLTRDLAGRSIRVAEHCYGCTAGQGS
                     SCGGALHD"
     gene            341..2506
                     /locus_tag="VN496_13950"
     CDS             341..2506
                     /locus_tag="VN496_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXF18108.1"
                     /translation="MHLKKILLVIAVAVLVIAFFALDLHHILTLDGLKASRERFETLR
                     SASPVLVTLAFFGFYVIVTAFTLPGAAVMTLAAGALFGLWWGTVIVSFASSVGATLAF
                     LGSRYLLRDTVQKRFGDRLKAVNEGIAKEGALYLLTLRLVPLFPFFLVNLLMGLTSIP
                     TRTYYWVSQSGMLAGTLVYVNAGTQLAQIDSLSGILSPGLLLSFALLGVFPWISQRFI
                     NYVKRRRVYARWRRPAKYDRNLVVIGAGAAGLVSAYIAAAVKAKVTLIEAHKMGGDCL
                     NYGCVPSKALIRSATLVHQIQHGERYGLEQAQPQFSFRQLMARVHAVIRAIEPHDSVE
                     RYTALGVEVLQGYARIVDPWTVEVALNGGGTQRLTTRSIVIAAGARPLVPPMPGLDDV
                     GYVTSDTLWDEFAKLDAPPARLVVLGGGPVGCELAQAFARLGSKVTQIELAARIMIRE
                     DMEISELARASLTRDGVHVMTGHKAVRCEHESERKFIVVEDGKQEHRVEFDVLLCAVG
                     RAARLTGYGLEALGIQTQRTVQTNDYLETIYPNIFAAGDVAGPYQFTHTAAHQAWYAS
                     VNALFGDFKKFKADYSVVPWATFIDPEVARVGLNEQEAREKGIAYEVTRYGLDELDRA
                     IADGEAHGFVKVLTVPGKDRILGATIVGAHAADLLAEFVLAMKHGLGLNKILGTIHTY
                     PTMAEGNKYAAGEWKRAHAPQRLLRWLEKFHGWKRGARS"
     gene            2503..3309
                     /locus_tag="VN496_13955"
     CDS             2503..3309
                     /locus_tag="VN496_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HXF18109.1"
                     /translation="MTRVIAAVLLALLWSGAQAATFDQSTWDAMLKAHVSSIRNGQST
                     QVDYAAFARDRSQLKQYLAGVSGVTQAEFEHWEPPAQLAFLINAYNAFTIDLVLTGYP
                     DVASIKDLGSLLRSPWKKRFIPLLGDTRSLDDIEHGLIRGSGRYNEPRVHFALNCASI
                     GCPALRPEAFSGERLDAQLEDAATAFLSDRTRNRLEGKALQVSSIFKWYRGDFEKGWR
                     GADNLAGFFLLYRQPLGLDDNAANRLKAGEIKIDFLDYDWRLNGKTTRTP"
BASE COUNT          644 a          989 c         1034 g          722 t
ORIGIN      
        1 acatgccgat ccagcgcttc ggctcgatgc tgatctcgaa ggggcagttc aatcaataca
       61 tgcacctctt gcgtgacaat ttcatggctg ccaatctttc caacgtcatg tgtcgcagcc
      121 tggtcagcat cgactggcag ggttatctct atgattgcga cttcaatcag atgttgggcc
      181 tcgcgctgcc ggagcagctt gttgacggcg cgcgtcccca tctgcgcgat ttactgacca
      241 gggatctggc ggggcgatcc atccgcgttg ccgagcactg ctacggctgt accgcagggc
      301 aaggcagcag ctgcggcggc gcgcttcacg actaaagcgg ttgcacttga aaaaaattct
      361 gctggtgatt gcagtcgccg ttttggttat cgcgtttttt gcgctcgatc tgcaccacat
      421 tctcaccctc gatggcctga aggcaagccg tgaacgcttt gaaacattgc gttccgcatc
      481 gccggtcctg gtcacgctgg ctttcttcgg cttctatgtg atcgtcacgg cgtttaccct
      541 gccgggcgcg gccgtcatga ctctggcggc gggcgcgttg tttggtttgt ggtggggaac
      601 cgtgattgtt tcttttgcat cgagcgtcgg ggcaaccctc gctttcctag gctcgcgcta
      661 cttgctgcgc gacacggtgc agaagcggtt tggtgaccgt ctcaaagccg tcaatgaggg
      721 aattgccaag gaaggcgcgc tgtacctgct gacgttgcgt ctggtgccgc tgtttccgtt
      781 cttcctcgtc aatctgctga tggggctgac ctcgattccg actcgcactt actattgggt
      841 tagccagtct ggaatgctcg ctggaaccct ggtgtatgtg aatgccggca cgcaattggc
      901 gcaaatcgac agcctgtccg gcatcctttc accggggctg ctgttgtctt ttgcgctgct
      961 cggcgtgttt ccatggatct cccagcgctt cattaattat gtgaaacgcc gtcgcgtata
     1021 cgcacgctgg cggcgtccag caaagtacga ccgcaatctt gtcgtaatcg gcgccggcgc
     1081 agccggtctg gtcagcgcgt atatcgctgc tgccgtgaaa gctaaagtca cgctgattga
     1141 agcacacaag atgggcggcg actgcctgaa ttacggctgc gtgcccagca aggcactgat
     1201 ccgcagcgcc acactggtcc atcagataca gcatggtgag cgctatggat tggaacaggc
     1261 gcaaccgcag ttcagtttcc gccagctcat ggcgcgcgtg catgccgtca tccgtgccat
     1321 cgagccgcat gacagcgtgg aacgctacac ggcgctcggc gtggaagtgc tgcaaggcta
     1381 cgcgcggatc gtcgatccgt ggacggtcga ggttgcgctg aacggcggtg gcacgcagcg
     1441 cctgaccacg cgcagtattg tcattgccgc tggtgcgcgt cctctggtcc cgcccatgcc
     1501 cggtcttgac gacgtcggct acgtgacgag cgacacgctc tgggacgaat tcgccaagct
     1561 ggatgcaccg cctgcacggc tcgtcgtact cggcggcggt ccggtggggt gcgaactcgc
     1621 gcaggcgttt gccaggcttg gctcgaaagt cacgcagatc gaattggcgg cacgcatcat
     1681 gatccgcgaa gacatggaaa tctcggaact cgcgcgcgct tcgctgacgc gtgacggcgt
     1741 gcacgtaatg accgggcaca aggccgtgcg ctgtgaacac gagtcagagc gcaagttcat
     1801 cgtagtcgaa gacggcaagc aggaacatcg ggtcgaattc gatgtgctgc tctgtgctgt
     1861 tgggcgcgcg gcgcggctca cgggttacgg acttgaggca ttgggcatcc agacacagcg
     1921 cacagtgcag accaacgact accttgaaac gatatacccg aatatctttg ctgccggaga
     1981 tgtcgccggg ccgtatcagt ttacgcacac cgcggcacat caggcctggt atgcctcggt
     2041 gaacgcgctg tttggcgact tcaagaaatt caaggccgat tactcggttg tgccttgggc
     2101 gactttcatt gatccggaag tcgcccgcgt gggactcaac gaacaggagg cgcgcgaaaa
     2161 gggcattgca tatgaagtca cgcgctacgg cctggatgaa ctggaccggg ctattgctga
     2221 cggcgaggcg cacggcttcg tgaaggtttt gaccgtgccg ggcaaggatc gcattctggg
     2281 cgccaccatc gtcggcgcgc acgcagccga cctgcttgcg gagttcgtgc tggccatgaa
     2341 acacggactc gggctcaaca agatcctggg cacgattcac acctatccga ccatggcaga
     2401 gggtaacaaa tacgctgccg gcgaatggaa gcgcgcgcac gcgccacagc ggctgttgcg
     2461 ttggctggaa aaattccacg ggtggaaacg cggagcgcga tcgtgacacg cgtgatcgcc
     2521 gccgtgctgt tggcgttgct gtggtccggc gcgcaggccg ctaccttcga ccaatcaaca
     2581 tgggacgcca tgctcaaagc gcatgtcagc tccatacgca atggccagtc aacccaggtt
     2641 gactatgccg cgtttgccag ggatcgcagc cagctcaagc agtatctggc cggcgtgtcg
     2701 ggcgtgaccc aggctgaatt cgaacattgg gagccccccg cgcagctggc gtttctgatc
     2761 aacgcctaca acgcttttac aatcgatctg gtattgaccg gctacccgga tgtcgcttcg
     2821 atcaaggatc tcggctcgct gttgcgctca ccgtggaaaa agcgttttat tccattgctg
     2881 ggcgataccc ggtcgctgga tgatatcgag catggactga tccgcgggtc cggccgctac
     2941 aacgaaccgc gtgtgcattt cgcgctgaat tgcgcgagca tcggctgccc ggcgcttcgg
     3001 cccgaagcat tcagcggtga acggctcgat gcccaattgg aagacgcggc caccgccttt
     3061 ctgtcggatc gcacgcgcaa tcgcctggag ggcaaagcgc tgcaggtctc cagcattttc
     3121 aaatggtatc gcggtgactt cgagaagggc tggcgcggag ccgataatct cgcaggattt
     3181 ttcctgcttt atcggcagcc gctcgggctc gacgacaacg cagccaatcg cctcaaggcc
     3241 ggtgaaatca aaattgattt tctggactac gactggcgat tgaacggaaa aacgacgcgc
     3301 acgccatgaa cctgtccgtg atcgtgccga tcctcaatga agcacaggcg ctggcaagcc
     3361 tgctcgatca actggcgcgc ctcgcgcat
//
LOCUS       DATMZQ010000437         6755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5012832, whole genome shotgun sequence.
ACCESSION   DATMZQ010000437 DATMZQ010000000
VERSION     DATMZQ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5012832"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..143
                     /locus_tag="VN496_13960"
     CDS             9..143
                     /locus_tag="VN496_13960"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HXF18110.1"
                     /translation="MPAAGLPKNIAVGDTVAFEFHQTGDGAYELTSITPLPAATGGKK
                     "
     gene            140..3316
                     /locus_tag="VN496_13965"
     CDS             140..3316
                     /locus_tag="VN496_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF18111.1"
                     /translation="MIAGLIRWSIANRFLVLLATLMVLVWGVWALSKTPLDAIPDLSD
                     VQVIIRTTYPGQAPQIVENQITYPLTTTMLSVPGAKTVRGYSFFGDSFVYILFDDGTD
                     PYWARSRVLEYLNQVQSRLPPQAKTALGPDATGVGWIYEYALVDRSGKMDLSQLRALQ
                     DWFLKYELKAVENVAEVASIGGMVRQYQIVLDPDKLRAYNIAHGKVIEAVRRANQETG
                     GSVLELGEAEYMVRASGYLQTLDDFRKIPLMSTDAGVSVRLGDVAQVQIGPEMRRGIA
                     ELNGEGEVAGGVIVMRSGKNALQTINAVKAKLDTLKPGLPPGVEIVPVYDRSSLIKRA
                     VANLREKLIEEFIVVAIVCVVFLFHIRSAFVAIVSLPLGILIAFIIMYYQGVNANIMS
                     LGGIAIAVGAMVDAAVVMIENAHKHIEAWNHAHPGEHLNGVAHWQVIGDAAAEVGPAL
                     FFSLLIIVLSFVPVFSLQAQEGRLFSPLAFTKTYAMAAAAGLAVTLIPVLMGYLIRGR
                     IPEERRNPLNRFFIAAYRPVLNGVLRYPMATLVIALLVTLLSVWPLSRLGAEFMPPLD
                     EGDLLYMPSALPGISAGKVAEVLQQTDRMIKSVPEVASVFGKAGRAETATDPAPLEML
                     ETTIQFKPRAEWRPGMTTAKLVEELDRIVKVPGLTNIWVPPIRNRIDMLATGIKSPVG
                     VKVAGTSLGEIDRIAAEIERVVRRVPGVSSALAERVTGGRYVDVDINRDAATRYGLNI
                     ADVQSIVAAAIGGDNIGETVEGLQRFPINLRYPRETRDSVEKLRQLSILTERGAQIRL
                     GDVANIRISDGPPMIKSENARLSGWIYVDIRGRDLSSAVREMQQAVTQEVKLPAGYSI
                     SWSGQFEFLERAASRLKIVVPGTLLIIFVLLYLTFQRIAEAVLIMSALPFALAGGVWL
                     LWILGHHVSVASAVGFIALAGVAAEFGVIMLLYLQRAWEARLLAGKMTETDLLDAIRE
                     GAVLRVRPKAMTVAVIIGGLVPIMIGTGTGSEVMQRIAAPMVGGMVTAPLLSMLVLPA
                     AYYLVHRRRAVNRSGQPAALINAKRKEN"
     gene            complement(3338..3679)
                     /locus_tag="VN496_13970"
     CDS             complement(3338..3679)
                     /locus_tag="VN496_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18112.1"
                     /translation="MALLLTGQQTVFAQTDVEADPAHHKLLFENEQVRVVRAIFGAGE
                     KSTAPFDAKDVVVVRVKGSGPITINIPYGAPITADSMEPGDVAWAPAGRIQPENKTDQ
                     RLEFIVIEPKH"
     gene            complement(3793..4704)
                     /locus_tag="VN496_13975"
     CDS             complement(3793..4704)
                     /locus_tag="VN496_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXF18113.1"
                     /translation="MTILVGTASWTDKSLIESGKFYPRDATTPEARLRYYARQFPLVE
                     IDSSYYGIPLPATAQLWAERSPPGFVFNVKAFRLFTGHQTLPKVFPRDIQEELGLPLT
                     KNIYYEDIPGEIRRELWNRFFDALDPLRRTGKLGAVHFQFAPWITSGGEPRKHVEHCV
                     STMNGLLMAVEFRNSSWWTEKTRDSTLAFEREHRVVNVVVDGPQGFGSSVPSVWEVTS
                     PELAIVRMHGRNAETWEKKGLKASSDRFNYDYSEEELRELAAKVHALAAQVPTVHAIM
                     NNNYQDQGQRNAKTLMSALQNLQPQSG"
     gene            4925..5458
                     /locus_tag="VN496_13980"
     CDS             4925..5458
                     /locus_tag="VN496_13980"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF18114.1"
                     /translation="MNNFARAILLVLVWFATQTLGADGNELSARQSIRINAAPETVWA
                     VVGNFNGTPRWLPLVVKSEIVLGTNNRVGAIRLVTRRNGTKVTERLLDRDPHDMHMAY
                     TYVDGAVMATDYSPVLSVKDAGDGTSIVEWSAHFKPLAYSNEATSAQDERALTDFFDG
                     VYKTGLDNLKRVVESGE"
     gene            5495..6736
                     /locus_tag="VN496_13985"
     CDS             5495..6736
                     /locus_tag="VN496_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXF18115.1"
                     /translation="MKRTMWSKLVCTIAMSLLGTWAMAADTIKIAHIDPLSGPFALVG
                     DSLSRLLQAAIDEVNSKGGVLNGTKLELVNFDSKGSPQESVLMLKQMIDSGIHYVSLG
                     SGSHIAHAMIESINKHNSRNPDQAVVLLNIAAQDPLLTNEKCSFWHFRWEANTDMRVN
                     ALTDFIVKQKGTPAVYLINQDYAFGQAISRAAKEMLAAKRPDVRVVGDDLHPLGKVKD
                     FAPYVAKIKASGADMVLTGNWGPDLSLLIKASKDAGLDVVYYTLNANNVGVLGSIGKA
                     GGDHIRQILTWHANVADNKLEKFANDYKKRFSEDMWVNLPKLQIDMLAKAIDTAQSAD
                     PIKVAMALHGMKFQSETGEAWMRMEDHQLIVPQYIATFTKTGGAVKYDAEGTGDGWKT
                     DVRIEGKDMAMPTSCKMEVPR"
BASE COUNT         1430 a         1826 c         2016 g         1483 t
ORIGIN      
        1 gcttcggcat tccggccgca ggtctgccaa aaaatattgc ggtcggcgat accgttgcgt
       61 tcgagttcca ccagaccggg gacggagctt atgaactgac ttccataaca cctttgccgg
      121 cggctacggg tggaaaaaaa tgattgccgg tctgattcgt tggtctatcg ccaaccggtt
      181 tttggtgcta cttgcgacac tcatggttct ggtatggggc gtctgggcct tgtcgaaaac
      241 gccgcttgac gccattcccg acctttcgga tgtgcaagtc atcatccgta ccacgtatcc
      301 gggacaggca ccgcagattg tcgagaacca gatcacgtat ccgctgacga ctacgatgct
      361 ttccgttccg ggagcaaaaa ctgtccgcgg ttattcgttc tttggcgact ctttcgtcta
      421 catcctgttc gacgatggca cggacccgta ctgggcgcgc tcgcgcgtgc tcgaatatct
      481 gaatcaggtg cagtctcgtc tgccgcctca ggctaagacg gcactggggc cggatgcaac
      541 cggagttggt tggatttacg aatatgcgct ggtagatcgc agcggcaaga tggatttgtc
      601 gcaactgcgt gctttgcagg actggtttct gaagtatgaa ttaaaggcag tggaaaatgt
      661 ggcagaagtc gccagcattg gcggcatggt tcgccaatac cagatcgtgc tcgatccgga
      721 caaactgcgc gcgtacaaca ttgcgcacgg caaagttatc gaggcggtcc gccgtgcgaa
      781 tcaggaaacg ggtggttcag tgctggagct cggcgaggct gagtacatgg tccgcgcatc
      841 cggctatctg cagacactcg acgatttccg caaaattccg ttgatgagta ctgatgccgg
      901 tgtgtcggtg cgcctgggag acgttgcgca agttcagatc ggtccggaaa tgcgccgagg
      961 cattgcggaa ttgaacggcg agggtgaagt ggcgggcggc gtgatcgtga tgcgctccgg
     1021 taaaaatgca ctccagacca tcaatgccgt aaaagcgaag ctcgacacgt tgaagccggg
     1081 attacccccc ggcgtcgaaa tcgttccggt ctacgaccgg tccagcctga tcaagcgtgc
     1141 agtggccaat cttcgggaaa agctgatcga ggaatttatc gtcgttgcta tcgtttgcgt
     1201 ggtttttctg tttcatattc gctcagcatt cgtggcgatt gtttcgcttc cgctgggcat
     1261 cctcattgca ttcatcatca tgtactacca gggggtgaac gcaaatatca tgtcgctagg
     1321 cggtattgcg attgccgtgg gcgccatggt ggacgctgct gtggtcatga tcgaaaatgc
     1381 acacaagcat atcgaggcct ggaaccatgc gcacccgggc gagcatctga acggggtggc
     1441 ccactggcag gtgatcggcg acgcggccgc cgaagtcgga ccggcactct tcttctcgtt
     1501 gctgatcatc gtgctgtcct ttgttcctgt cttcagcttg caagcgcagg aaggccggct
     1561 attttccccg cttgcattta ccaagactta cgcgatggcg gcagcagccg gacttgccgt
     1621 cacactcatt ccggtgctga tgggttatct gatccgtgga cgcatacccg aagaacgacg
     1681 caaccctctc aaccgattct tcatagcggc ttatcggccg gttttgaacg gggtgctgcg
     1741 ctatccgatg gcgacattgg tcatcgcgct cttggtcacc ttgctcagcg tttggccgtt
     1801 gagccgcctg ggcgcagaat tcatgcctcc gctcgacgag ggcgatttgc tttacatgcc
     1861 gtccgcattg cccggtatct ctgccggcaa ggtggccgag gtcttgcagc agaccgaccg
     1921 catgatcaaa agcgtgccgg aagttgcgag cgtattcggc aaggctggcc gtgccgagac
     1981 tgcgaccgac ccggcgccac tggaaatgct ggagaccacg atccagttca agccgcgggc
     2041 tgagtggcgg ccgggcatga cgaccgccaa gctggtcgag gaactcgatc gaatcgtgaa
     2101 agtgccgggg ctcaccaata tctgggtgcc gcccattcgt aaccggatcg acatgctggc
     2161 gaccggtatc aaaagtccgg tcggcgtgaa agtcgccgga accagcctgg gggagatcga
     2221 tcgcatcgct gcagagatag agcgggttgt aaggcgagtg ccgggcgttt cttccgcact
     2281 cgcggaacgg gtgactggcg gccgctacgt cgacgtagac atcaaccgtg atgcggctac
     2341 gcgctacggc ttgaatatcg cggacgtaca gagcatcgtc gcagcggcga tcgggggcga
     2401 caacatcggt gaaaccgtag aaggacttca gcgctttccg attaatctgc gctatccccg
     2461 cgagacacgc gattcggtcg agaaactaag acagctgtcg atattgacgg agcgcggcgc
     2521 gcaaatccgc ctgggcgacg tcgccaatat ccgcatcagt gacggaccgc cgatgatcaa
     2581 aagcgagaac gcgcgccttt cgggctggat ctatgtggac atccgaggcc gtgatctgag
     2641 ctccgcggtc cgggaaatgc agcaggccgt cacgcaggaa gtcaagctgc cggccggcta
     2701 ttctatttcc tggtcggggc agttcgaatt cctggagcgt gccgcatcca ggctcaagat
     2761 cgttgtgccc ggcacgctct tgataatctt tgtactgctt tacctgactt ttcagagaat
     2821 cgccgaggca gtcctgatca tgtcagcgct tccgttcgcc ctcgcaggcg gcgtctggct
     2881 gctatggatc ctcggccatc atgtttccgt cgcgagcgct gtagggttta ttgcgctcgc
     2941 cggtgtcgcg gcggaatttg gcgtcatcat gctgttgtat ctccagcgtg cctgggaagc
     3001 gcggctgctg gccggaaaaa tgacggaaac ggacctgctg gatgcgattc gagaaggtgc
     3061 ggtactgcgt gtgcgaccga aagccatgac tgtggcggtg atcatcggcg gcctggtgcc
     3121 gatcatgatt ggcaccggca ccgggtcgga agtgatgcag cgcattgcgg cgccgatggt
     3181 gggcggaatg gtcaccgcac cgctattatc catgctggta ttgccggcgg cttattacct
     3241 ggtgcatcgg cggcgcgccg tgaatcgtag cgggcaacca gctgcattga taaacgccaa
     3301 acgcaaggag aactgactgc cttgccgtgg aaccgcgtta atgctttggt tcgattacga
     3361 tgaattcaag tcgctgatcc gttttgttct caggctgaat ccttcctgca ggagcccaag
     3421 ccacatctcc aggctccatt gaatccgcgg taatgggcgc accatatgga atattgatgg
     3481 ttatcgggcc acttcccttc actctgacta ccaccacgtc tttagcatcg aacggcgcgg
     3541 ttgatttttc tcccgcgccg aaaatggcgc gcacgactct tacctgttca ttctcgaaaa
     3601 gcaatttgtg gtgggcagga tccgcttcca catcggtctg cgcaaaaacg gtttgttgcc
     3661 cagtcagcaa caaagccacg acgcttggta cgatcaaccg ggacataaag cgcatcattt
     3721 gcctccttgt gcagaatttt gctcgttcgt tgctgatggg tgacccggat agcatagtcc
     3781 aaaagcggat gattaaccgg attggggttg caggttctgc agagcgctca tgagcgtctt
     3841 tgcgttgcgc tgcccctgat cctgatagtt gttattcatg atggcgtgga ccgttggcac
     3901 ctgtgctgcc agcgcgtgga ccttggcggc gagttcgcgc agttcctcct cactgtagtc
     3961 atagttgaat cggtcggacg aagctttcag ccctttcttc tcccatgtct ctgcattccg
     4021 gccatgcatc ctgacgatgg cgagctcagg agacgtcact tcccagaccg acggcacgct
     4081 gctgccaaat ccctgaggtc cgtcaacaac cacgttgacg acgcgatgct cacgttcgaa
     4141 agccagggtc gaatcgcgtg tcttttcggt ccaccagctg ctgttcctga attccacagc
     4201 catcaacagt ccattcatgg tcgagacgca atgctccacg tgcttccggg gctctccacc
     4261 ggatgtgatc cagggcgcaa attgaaaatg cacggcgcct aattttccgg tccgccgcag
     4321 cgggtccagc gcgtcaaaga agcggttcca tagttcgcgc cggatttccc ccgggatgtc
     4381 ctcataatag atgttcttcg tcagaggcag gcccagctct tcctgaatgt cccgcgggaa
     4441 aaccttcggc agtgtttgat gaccggtaaa aagccggaac gctttgacgt tgaatacaaa
     4501 gccggggggc gagcgctcgg cccacaactg cgcagtcgca ggaagcggaa tgccgtagta
     4561 actggagtct atctccacga gggggaactg ccgggcgtaa tatctgagcc gcgcctctgg
     4621 cgtcgtcgca tcgcggggat agaacttgcc cgattcgata aggctcttgt cggtccagga
     4681 tgccgtgcct accaggatag tcatcgtgtc atcaacgttg gaactcgtga tctggcaaat
     4741 gtagcagcgc gtctgctatt tgtcgtactg accctgttct ggcggaggtg ttcagaacaa
     4801 cgcaagggtc aggcaatgct tcagcaggct ctcgcctgtg cattcgtctc ggaggatagt
     4861 aggcgccgcg cccgatttgg aatatcgtcg caattgggcg aataatcata aaaggggagg
     4921 gaatatgaat aacttcgcta gagcaatatt gctggttttg gtttggttcg ctacccaaac
     4981 tcttggagcc gacggtaatg aactcagtgc gcgtcaatca atccggatca acgcggcgcc
     5041 agagacggta tgggcagtcg tgggaaactt caacggaaca ccacgctggc tgccgttggt
     5101 cgtgaaaagc gaaatcgtgc tgggcacgaa caatcgtgtc ggcgctatcc ggttggttac
     5161 acggcgaaac ggcaccaagg tcacggagcg gttgctggat cgcgatccac acgacatgca
     5221 tatggcttac acctatgtgg atggcgcagt catggcgacc gactattctc ccgtcttgag
     5281 cgtcaaggac gcgggggatg gaaccagcat cgttgaatgg agcgcgcatt tcaagccttt
     5341 ggcctattcg aacgaggcga cttctgcaca ggacgaacgc gcattgaccg acttcttcga
     5401 tggcgtctat aagactgggc tggacaactt gaagcgcgtg gtggagagcg gcgagtagtt
     5461 cttcagatct ggaattgcgg aaaggaatac gaatatgaaa cgcaccatgt ggtcgaagct
     5521 cgtgtgcacc atcgcgatgt cgcttcttgg gacctgggca atggctgccg ataccatcaa
     5581 gattgcgcat atcgatccat tgagcggtcc gtttgcgctg gtgggcgaca gcctcagccg
     5641 gttgctgcag gctgcgatcg acgaagtcaa ttcaaagggc ggtgtactca acggcacgaa
     5701 gctcgagctg gtgaacttcg acagcaaagg cagcccgcag gaaagtgtgc tgatgctgaa
     5761 acagatgatc gattcgggga tccactacgt ttcccttggg tccgggtcgc acatcgcaca
     5821 tgccatgatc gaatccatca acaagcacaa cagccgcaat cctgatcagg cggtggtgct
     5881 gttgaacatt gccgcgcagg atccgttact caccaacgag aaatgcagct tctggcattt
     5941 tcgctgggaa gccaacaccg acatgcgtgt aaacgccttg accgacttta ttgtgaagca
     6001 aaagggcaca cccgcggttt acctgatcaa ccaggattac gcgttcggac aggccatcag
     6061 ccgcgctgca aaggaaatgc tggcagcgaa gcggcccgat gtcagagtcg tgggcgacga
     6121 cctgcatccc ttgggtaagg tgaaggattt tgcgccgtac gtcgccaaga tcaaggcgtc
     6181 gggggcggac atggttctga caggcaactg ggggccggat ctttccctcc tgatcaaggc
     6241 aagcaaggac gccggccttg acgtcgtcta ctacacgctg aacgcgaaca atgttggcgt
     6301 gctgggctcg attggaaagg cgggtggtga ccacatccgc caaattttga cctggcatgc
     6361 gaatgtagcg gataacaagc tggagaaatt tgccaacgac tacaagaagc gtttcagcga
     6421 agacatgtgg gtcaatctgc cgaaactgca gatcgacatg cttgcgaagg ctatcgacac
     6481 agctcagtcc gccgatccaa tcaaggtcgc catggcgctg cacggcatga aattccagag
     6541 tgaaaccggc gaggcgtgga tgcggatgga agatcatcaa ttgattgttc cccagtacat
     6601 cgccacattt accaagaccg ggggcgccgt aaagtacgac gcggaaggaa ccggtgacgg
     6661 ctggaagacc gatgtgcgaa tcgagggcaa ggatatggcc atgcctacga gctgcaagat
     6721 ggaggtaccg cgctagccag aactgctttc ttctg
//
LOCUS       DATMZQ010000438         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3637979, whole genome shotgun sequence.
ACCESSION   DATMZQ010000438 DATMZQ010000000
VERSION     DATMZQ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637979"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..294)
                     /locus_tag="VN496_13990"
     CDS             complement(58..294)
                     /locus_tag="VN496_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18116.1"
                     /translation="MLDVSRLNRSFVRMDRAASRLTMGIVTASLIIGSSIVMTVKTES
                     TWLGLPLFGLMGFLGAVICGIWLLISIVRSGRSD"
     gene            530..817
                     /locus_tag="VN496_13995"
     CDS             530..817
                     /locus_tag="VN496_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18117.1"
                     /translation="MYHAVVWIDHREAHVESFTRDASETSYVKALGNPRKIHHRGGSV
                     EGGKAPVNLQFFALVGDTLNGAREALNMRAAVSGQRIECSSNRDPVTFNPS"
     gene            814..1524
                     /locus_tag="VN496_14000"
     CDS             814..1524
                     /locus_tag="VN496_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXF18118.1"
                     /translation="MTVTWMILGAALLTGLLGGVHCAGMCGGIVTALVGRSAGNTRPW
                     AMHLAYNAGRIVSYAFAGAAAGAAGSLGLMLDAWLPVQVILYALANILLVALGLYLAG
                     LRSPITRLEHLGMGLWRRIQPLTRHFLPADSVPKALMLGMLWGWLPCGLVYTVLFTAL
                     LSGNAVNGAILMLAFGLGTLPNLMAAAVFLQRSRSLLAGRMARMLSGATVMAFGVYGI
                     AHAGSLAAQIRGGLLCIG"
     gene            1680..2276
                     /locus_tag="VN496_14005"
     CDS             1680..2276
                     /locus_tag="VN496_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF18119.1"
                     /translation="MIAEDPAFWRAGAISSTLVMLVVLGFLTVDSLATIRPGGANVPA
                     YTVINKEIRYEFDVESNRYRPVIGAEQLLFGRNYSAQEAEETMRLGKLVIQSRNCMNC
                     HTFFGNGAYYAPDLTKAWLDPAWQQMWMPMTQSSTREDAMAIFLMNPEKFPTWSRQMP
                     NLNITRKEAEALVAYLKWMSAVETNGFPIHFGHIQTNR"
     gene            2286..>2635
                     /locus_tag="VN496_14010"
     CDS             2286..>2635
                     /locus_tag="VN496_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXF18120.1"
                     /translation="MFRSQQLALRYFAAAMTLFGIMIVAGLLASTHYVQDGFMLNKLD
                     FSITKTLHIDTMIIWLLMGFIGAVYWFLPIELEREVEGIKLAEIMFWIFCAAVAVVAA
                     VFIFVQYGPATELSL"
BASE COUNT          507 a          778 c          800 g          550 t
ORIGIN      
        1 tgggaggaaa atggaagtgc agcgcacatc caggctaggc gcgtcgaacc aaagggacta
       61 atcgctgcgg ccgctgcgta cgatcgaaat cagcagccat atgccgcaga tgaccgcacc
      121 gagaaaaccc atcagtccga acaacggcaa gccgagccat gtcgattctg tttttaccgt
      181 catcacgatg gaagagccga tgatgagcga ggccgtgacg atacccattg tcagacggct
      241 tgccgcccgg tccatgcgca cgaaggagcg gttcaggcgc gaaacgtcca acatcgctaa
      301 gccctgttat tcatggaatt tgccgaactt atgtacagat tgtacggcag ggggacgggc
      361 agccgttgat ccggctcaat gcgctttgtc gcttcgactt gatccggatc aacatgcaga
      421 caacccggcg cgggaagaat tcgtgtgtga gttttaagcg attgccgttc ccgacgcggc
      481 ggcgatcagc gtcaatgcaa tcgtccatgt gtgaggaaac cgccaatgcg tgtaccacgc
      541 cgtagtctgg atcgaccatc gtgaggccca cgtcgagagc ttcacgcgcg acgcaagcga
      601 aacctcctac gtcaaggccc ttggcaaccc gcgcaagatc catcaccgcg gcggttcggt
      661 cgaaggaggc aaggcgcccg tgaaccttca attctttgcg ctcgtgggcg acacgttgaa
      721 cggggcgcgg gaggccctga acatgcgcgc cgctgtttcc gggcagcgga tcgaatgctc
      781 atccaaccgc gatcccgtca cgtttaatcc ctcatgacgg tcacctggat gattctgggc
      841 gcggctctgt tgaccggatt gctcggaggt gttcattgtg ctggcatgtg cggcggcatc
      901 gtcacggcgc tggtgggacg atcggccgga aacaccaggc cttgggccat gcatctcgcg
      961 tacaacgccg gacgtatcgt cagctatgcg ttcgcgggcg cggcggcagg ggccgcgggc
     1021 agtctcggcc tgatgctcga cgcatggctg ccggtacagg tcatactcta tgcgctggcg
     1081 aatattctcc ttgtcgcgct gggtctgtat ctggccggcc tccgcagccc catcacgcgc
     1141 ctggaacatc tgggcatggg actgtggcgg cgcattcagc cgctaacgcg ccacttcctg
     1201 cccgcggatt ccgtacccaa ggcgctgatg ctcggcatgc tttggggctg gttgccgtgc
     1261 ggcctcgtct atacggtttt gttcacggcc ctgttgagcg gcaacgcggt caacggcgcg
     1321 atcctgatgc tggcctttgg cctgggcacc ttgcctaacc tgatggcggc cgctgtcttc
     1381 ctacaaaggt cgcgctcgct gcttgccggc agaatggcgc gcatgttgtc cggggcaacc
     1441 gtgatggcat ttggcgtcta cggcatcgcg cacgccggca gcctggccgc gcagatcaga
     1501 ggcggattgc tctgtattgg ctaagcgcga tgccggggca tgggcggtcg cgcgaattgc
     1561 attcccttga tctatatcaa atccgctcac gcaattcatc cgaccatggc gcaacgtctg
     1621 gcatgggccg gataggctgc ccggatttat ccatcagcat ttgaggataa ggaggatcta
     1681 tgatcgctga agaccccgcg ttctggcgcg ccggtgccat cagctcgacg ctcgtcatgc
     1741 tggtcgtgct tggctttctg acagtggata gcctcgccac catccgtcct ggtggcgcca
     1801 atgttcccgc ctacaccgtc atcaacaagg aaatacgtta cgagttcgac gtcgagtcca
     1861 atcgctatcg gccggtgatc ggtgcggagc aattgctgtt cgggcgcaac tattccgcgc
     1921 aggaagccga ggagaccatg cgcctcggta agctcgtgat ccagagccgg aactgcatga
     1981 actgccatac gttcttcggc aacggcgcct attacgctcc ggacctcacg aaggcgtggc
     2041 tggatcctgc ctggcagcag atgtggatgc cgatgacgca gtcgtcgacc cgcgaggacg
     2101 ccatggcgat tttcctgatg aacccggaaa aattccctac gtggagccgg cagatgccca
     2161 acctgaacat cacgcgcaag gaagctgaag cgctcgtcgc gtatctcaaa tggatgtcgg
     2221 cggtggagac caacggcttc ccgattcatt tcggccacat ccagaccaat cgataggagg
     2281 acgacatgtt ccggtcccag cagttggcgc tcaggtactt cgcggcggcg atgacgctgt
     2341 tcggcatcat gatcgtggcc gggctgcttg catcgaccca ttatgttcag gacgggttca
     2401 tgctgaacaa actggacttc agcataacca agacgctgca catcgacacc atgatcatct
     2461 ggctgctgat gggcttcatt ggcgcggtgt actggttcct gcctatcgaa ctggagcgcg
     2521 aggtcgaggg catcaaactt gccgagatca tgttctggat cttttgcgcg gcggtagccg
     2581 tggtcgctgc cgttttcatt ttcgtccaat acggcccggc aaccgaattg tcgct
//
LOCUS       DATMZQ010000439         8405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_878042, whole genome shotgun sequence.
ACCESSION   DATMZQ010000439 DATMZQ010000000
VERSION     DATMZQ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878042"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..565
                     /gene="tpx"
                     /locus_tag="VN496_14015"
     CDS             65..565
                     /gene="tpx"
                     /locus_tag="VN496_14015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HXF18121.1"
                     /translation="MTTVTLKGKPVTVSGSLPKKGETALDFSLANKDLADVTLASYAG
                     KRKVLNIVPSLDTPTCQQSTRVFNQKASSVKDSIVLVISADLPFAMSRFCSAEGLNNV
                     VTLSTFRCKDFHAKYGVDIVDGPLRGLAARAVVVLDQTNKVVHSELVPDIANEPDYEA
                     ALASLK"
     gene            641..1024
                     /locus_tag="VN496_14020"
     CDS             641..1024
                     /locus_tag="VN496_14020"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HXF18122.1"
                     /translation="MKSTQQESPHKGKTGLRRIGNAVLYSFAGLAAAYRHEDAFRQEL
                     LLACLLIPIALILPASGPGKALMIASVLLVLIVELLNSAVEATVDRVSLDHHLLAKRA
                     KDIGSAAVMISLANVIAVWSLVLLG"
     gene            1170..3725
                     /locus_tag="VN496_14025"
     CDS             1170..3725
                     /locus_tag="VN496_14025"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXF18123.1"
                     /translation="MKKTALWKADWFLGSVVALVLFLASTSDLVQGLERLAYDLGVRA
                     SNHAPSARIAVIAIDDQSVANIGRWPWSREVHAQMITMLKQAGAKVIGYASFFFEPQI
                     DPGLAYIERLTQLYQALPPPQQQSLLLFSDVLGEAETALNNDRKLAAAITEAGNVVLP
                     MLFVIGEPRGNPDRSLPDYVLKNQITKVLDRIGAQGNNIFPVPTVQVSVPIHEVAAGA
                     AVIGHLNALPDVDGSTRTEPLVVRYYDQYFPSFSLMLAAISLNLSPRDIEMRLGEGIK
                     FGKGSIATDPYLQMHNYFYKDRDGKPAFAPDSFYDVISGRIPAEKYRDKVVLVGATAS
                     GVGTYQVTPISPAMPPVITLAHAVSSLLQEDFFTTPTWGFWVEKIAFVSVALYIILLL
                     PRLNAGMGFTFSVAVLALLIGTHFFLMAAKLMWIQLMASAVLLVIGHMLLTTKRFLMT
                     ERGKARADLESAESNRMLGLAFQGQGQLDIAFDKFKKVPLEDSVMDNMYNLALDFERK
                     RQFNKAQTVYEYMSTYNPNFRDLNQKLDRARKMSETIILGAGGPRTNASTLLLDAAAV
                     EKPMLGRYQVEKELGKGAMGVVYLGKDPKIGRVVAIKTMALAQEFEAEELEEVKQRFF
                     REAETAGRLNHPNIVTIYDAGEEHDLAYIAMEFLKGSDLVPQTKQAALLALPTTLSII
                     ARVAEALSYAHAQNVVHRDVKPANIMYDAQTDSVKVTDFGIARITDSSKTKTGMVLGT
                     PSYMSPEQLGGEKIEGQSDLFSLGVTLYQLACGSLPFQGDSMTQLMYKIANEPPTDIR
                     TFNPALPKCLVEIIGKALAKKVTERYQNGEQFAQALRACAVLFHTVDVARQEY"
     gene            3727..4551
                     /locus_tag="VN496_14030"
     CDS             3727..4551
                     /locus_tag="VN496_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXF18124.1"
                     /translation="MSLMDSLEIAKATHTGMVRSHNEDSIATDTEIGLVVLADGMGGY
                     NAGEVASGIATALISSEIREAMLNEAPAKIDTATGSSGATTLLRATIAKANASIFQSA
                     NSQPQYAGMGTTLVTALLFNSQLTIAHIGDSRCYRYRSGKLEQITRDHSLLQEQIDSG
                     FLTREAAKRSQNKNLVTRALGIEPTVEAEIHTYPAQEGDIYLVCSDGLNDMVEDDDIQ
                     MTLDALGANLELAAEQLVQMANDNGGRDNISVVLIKVKKDFPANEGLFAKFFSWFK"
     gene            4563..5384
                     /locus_tag="VN496_14035"
     CDS             4563..5384
                     /locus_tag="VN496_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXF18125.1"
                     /translation="MAKLILSLDGQLIKEFTLSKERTTIGRKPHNDIQIDNLAISGEH
                     AVIMTILNDSFLEDLGSTNGTLVNGQPVKKHFLQGNDIIEIGKYKLKYVNETPAIMSK
                     ADFEKTMVLRSPAASAPKPDAKALTDTQTNLEVPTKSSEMTASTPSTATPPADATLPG
                     IAAGAKPDPVTVAVAQSASTSSVTTPGGGTIQILSGPNAGRELSLAKPLTTLGKPGVQ
                     VAVIAKRPQGYFITHVEGAQFPVLNGKTLDSQAHQLGDHDIIELASVKMEFFLKP"
     gene            5488..7701
                     /locus_tag="VN496_14040"
     CDS             5488..7701
                     /locus_tag="VN496_14040"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXF18126.1"
                     /translation="MRIALGLVVVLFFAGHAARYYAIPFIDRLENIVYDARLRLTMAN
                     SVDPRIVIVDIDEKSLAVEGRWPWRRDKLAAMLDQLFDRYKVSIVGFDIVFAERDESS
                     GLGILRELGQRELKGDAHFQAVLNAVGPKLEFDRVFAEKLKNRAIVLGYYFSSVKSAD
                     GKGRSAGALPKPVLPAGTFKGKNIQFTNWVGYGANLEEFQQAARSAGHFNPWPDEDGV
                     IRRVPILAEFDGAYYESLSMAVVRLALGLPPVLPGFPEGKSGSKNYPGLEWIDLGPLR
                     IPVDNSVTALVPYRGKEGSFKYVSATDVLHGSTPLDELKNKIMLIGTTAPGLYDLRTA
                     PVASVYPGVEIHANLIAGMLDGSIKQRPPYVLGAEVLLLLFSGVAMALVLPLLNPLRA
                     TLLTIVVVAAVFAANVLIWTEGNLVLPLASGLLMLVMLFALNMSYGFFVESRAKRQIT
                     GLFGQYVPPELVDEMSKDPEAFSMEAESRELSVLFTDVRGFTTISEGLDPKELSRLMN
                     EFLTPLTRIIYKHRGTIDKYMGDCIMAFWGAPLDDPQHARNAVLAGLEMHRVLAGLQP
                     HFKQKGLPSIQIGVGVNSGRMSVGNMGSEIRLAYTVMGDAVNLASRLEGITKQYGIKM
                     IVGEATRAELPDLLFRELDRVKVKGKETSVAIYEPVGLPVEIEKPLQDELKLWGQALK
                     LYRIRDWDMAELQLINLTKMSPGSALYALFLDRIAHLRVNPPEEGWDGSWTFEKK"
     gene            7704..>8405
                     /locus_tag="VN496_14045"
     CDS             7704..>8405
                     /locus_tag="VN496_14045"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HXF18127.1"
                     /translation="MQIRILGCSGGIGGNLRTTSMLIDNDVLVDAGTGVGDLAITDLL
                     KIDHVFLTHSHLDHITSLPFLVDTIGPMRETPITVHCTEATRKILQEHIFNWKVWPDF
                     TEIPSKSSPCLCYSTFAVGDIVGLGGRKIRVLPANHVVPAVGFQFDSGEASLVFTGDT
                     TINDALWDEVNKISNLRYLVIETAFPNMERELAIASKHLCPSMLAEELAKLRRPTRVF
                     ITHLKPGAGTLTMQEV"
BASE COUNT         2056 a         2085 c         2273 g         1991 t
ORIGIN      
        1 aaattattca ccgcaaccaa acgcttgacc ataaaccgac ctttttattc agaaagggca
       61 ctacatgacg actgtaacgc tgaagggaaa gccagttacc gtgtcaggca gtctgccaaa
      121 gaagggcgaa accgctttgg atttctcact ggccaacaag gaccttgctg atgtaacgct
      181 tgcaagttat gccggcaagc gaaaagtcct aaacattgtt cccagccttg acacgcccac
      241 ttgccagcaa tccactcggg tattcaacca aaaggcgtct agtgtcaaag actccatcgt
      301 gctcgtcatt tcggctgatc tcccgttcgc aatgtcacga ttctgtagcg cagaggggct
      361 caacaatgtt gttacgttat ctactttccg ctgcaaggat ttccatgcca agtacggtgt
      421 ggacattgtg gatggaccgc ttaggggatt ggccgcacgc gccgttgtcg tgcttgacca
      481 aaccaataag gtcgtgcact cagaactagt gccggacatc gcaaacgagc cagattacga
      541 agcagccctt gcttcactaa agtgagcggg tcctgttgca aataccacga agcttcattt
      601 aacgccacgg ctctcgcctg ggggaatgat ggaaaagatt ttgaaatcga ctcaacaaga
      661 gagccctcat aaaggaaaaa ccgggctgcg ccgcataggt aatgcggtgc tttattcgtt
      721 cgcaggactt gcggcggcgt accggcacga agatgcattc aggcaggaac tcctgcttgc
      781 ctgtttgctt attccaattg cattgattct tcctgcgagc gggccgggca aagccctcat
      841 gattgcgtca gtgctgctgg tgttgatcgt ggagctgttg aattcagcgg tcgaagcaac
      901 ggtcgaccgg gtttctctgg atcatcattt gctcgctaaa cgggctaagg atatcggtag
      961 cgctgcagtc atgatttcgc tggcgaatgt catcgctgtg tggtcgctgg tactactggg
     1021 atgatcacca aaggaaacag ttgagtctat ttcgcccgaa agttaaaaaa atcatatact
     1081 tttggccgaa atatcgtaca atctctagct taaacgcctt aattagctag aaatcaaggg
     1141 cttcaggcat ccaatcaggg gtgggtggca tgaagaagac ggctctttgg aaagccgatt
     1201 ggttccttgg atcagtcgta gcactggtct tgtttttggc atcgaccagc gacctcgtcc
     1261 agggacttga acgcttggca tatgacctgg gcgtccgggc ttccaatcac gcgccttccg
     1321 ccaggattgc agtgatagcc attgacgatc aaagcgtcgc gaatatcggt cgctggccct
     1381 ggtcgcgcga ggttcatgcg cagatgatca caatgcttaa gcaagccggt gcgaaggtaa
     1441 tcggctatgc gtcgttcttt ttcgagcccc agatagaccc gggactcgca tacattgaga
     1501 ggctgacgca actctatcaa gcccttcctc ccccacaaca acaaagtttg cttctatttt
     1561 ctgacgtcct tggagaagcc gagactgcgc tgaacaatga tagaaagctc gcggcagcca
     1621 ttactgaagc gggcaatgtt gtattgccaa tgttgttcgt gatcggcgag cctcgcggca
     1681 atccagacag gtcacttccc gattatgttt taaagaacca gattaccaag gtcctggatc
     1741 gtatcggagc gcagggcaat aacatctttc ctgttcccac cgtccaagtc tcagtaccga
     1801 ttcacgaagt agcggctggg gccgctgtaa tcgggcattt gaacgcattg ccggatgtag
     1861 acggcagcac acgtaccgaa ccccttgtcg tacgttatta cgatcaatat tttccttcgt
     1921 tttccttgat gctggcggcc atcagcctga atctcagccc tcgggacatt gaaatgcgat
     1981 tgggcgaggg gatcaaattc gggaaaggtt ccatcgctac ggatccgtat ttgcagatgc
     2041 acaactactt ctacaaagac agggatggca agccggcatt tgcaccggac tccttttacg
     2101 atgtgatatc gggaagaatt cctgcggaga aatatcgcga taaagtggta ttggtaggag
     2161 ccaccgcgtc aggcgttggt acataccagg taactccaat atcgcctgct atgccgcctg
     2221 tgattacatt ggcgcacgcg gtatcgagtc tgcttcagga agatttcttt actactccga
     2281 cctggggttt ctgggtcgaa aaaattgctt tcgtctcggt tgcgctctat atcattctcc
     2341 tgctgccgcg cctgaatgcc ggaatggggt ttacctttag cgtggcggtg ttagcgctgc
     2401 tcataggcac ccacttcttt ttgatggcag ctaaactcat gtggattcag ctaatggcat
     2461 cggcagtatt gctggtgatt ggccacatgc tgttgacgac gaagcgattc ctgatgactg
     2521 agcgcggaaa ggccagagca gaccttgaat cagctgaatc aaaccgtatg cttggccttg
     2581 cattccaagg ccaaggtcag ctcgacatcg cgttcgataa gttcaagaaa gtcccgcttg
     2641 aagattccgt gatggacaac atgtacaacc tcgctcttga ttttgagcgc aaacgccaat
     2701 tcaataaggc gcagacggtc tacgagtaca tgtcaactta caatcccaat tttcgcgatc
     2761 tgaatcagaa gctcgatcgt gcacgcaaga tgtcagagac cataatcctt ggcgcgggtg
     2821 gccctcgcac caatgcaagc actcttttgt tggacgccgc tgcggtggag aagccgatgc
     2881 ttggtcgcta ccaagttgaa aaggaacttg ggaaaggtgc catgggtgtg gtctaccttg
     2941 gcaaggaccc gaagattggc cgtgtggttg caatcaagac catggctttg gcacaggagt
     3001 tcgaggccga ggaacttgaa gaagtgaagc agcgtttctt tcgggaggcg gagaccgccg
     3061 gtcggctgaa tcatccaaat atcgttacga tctacgacgc tggcgaagaa cacgatctag
     3121 cgtatatcgc gatggaattt ttgaaaggca gcgatcttgt gccgcaaacc aaacaagcgg
     3181 ctcttctggc cttacctaca acgctctcaa ttattgcccg cgtcgcagag gcactttcat
     3241 atgcgcacgc gcagaacgtg gtacatcgag acgtcaagcc ggcaaatata atgtatgacg
     3301 cgcaaacaga ttccgtgaaa gtgacggatt tcggaattgc acggatcaca gattcttcca
     3361 aaacgaagac cggtatggtg cttggtacac cgtcatacat gtctccggaa cagctgggcg
     3421 gggaaaaaat agaaggccaa tctgatttat tttcgctggg cgttacactt tatcaacttg
     3481 catgcggcag cttgccattc cagggtgatt caatgacaca actcatgtac aagatagcca
     3541 atgagccgcc gacagacatc cgcacgttta atcctgcctt gccgaagtgt ctggtcgaaa
     3601 taataggcaa agcgctcgcc aaaaaagtga ccgaacgcta tcaaaacggt gaacagttcg
     3661 cgcaagcact ccgcgcctgc gcggttctgt tccataccgt cgacgtggct cgtcaagaat
     3721 actgacatga gcttgatgga ttctctggag attgcaaaag caacgcacac ggggatggtc
     3781 cgttcgcata acgaagatag cattgcaacg gacactgaaa ttggcctcgt ggttctggca
     3841 gacggtatgg ggggttataa cgcaggcgag gtggcaagcg gaattgcaac agctctgatt
     3901 tccagcgaaa tacgggaagc catgttgaac gaggcacccg ctaagatcga tactgcgacg
     3961 ggttcgtccg gtgccacaac gttgcttcgc gcgaccatcg ccaaggctaa tgcgtcgata
     4021 ttccagtctg caaacagcca gccacaatat gctggcatgg ggactacgct ggttaccgcg
     4081 ttgttattta acagtcagct caccattgcg cacattggtg attcgcgttg ctaccgctac
     4141 cgatctggca agcttgaaca gataacccgg gaccattcgc ttttacagga gcaaatcgat
     4201 agcggtttct tgacgaggga agcggcgaag cgctcgcaga acaagaatct ggttacgcgg
     4261 gcccttggca ttgaacctac cgtagaggct gaaattcaca cctacccggc acaggaggga
     4321 gatatttacc tggtgtgctc cgatgggcta aatgacatgg ttgaggacga cgatatccag
     4381 atgactctcg acgcactcgg cgctaatctg gaactggcgg cggagcaact ggtgcagatg
     4441 gcaaacgaca acggcgggcg agataatatc tccgtggtac tgataaaggt gaaaaaggac
     4501 tttccggcga acgagggact gtttgcgaag ttcttttcct ggttcaaata aaggaagcgg
     4561 ctatggcgaa gttgatattg agtctggatg gtcagctgat aaaggaattt acgctgagca
     4621 aagagcgtac gaccatcggt cgcaaaccgc acaacgatat tcaaatcgat aatttagcta
     4681 tcagcggtga acacgcagta attatgacta tcctcaacga ctccttcctc gaggatctgg
     4741 gcagtaccaa tggcactttg gttaacggcc agccggtgaa aaagcacttc ttgcagggca
     4801 acgacattat cgagattggc aagtacaagc ttaaatacgt taatgagacg cctgcaatca
     4861 tgagcaaggc cgattttgag aagaccatgg tgttgcgatc tccagccgca tcggcgccga
     4921 agccggacgc caaagccctc acagacaccc aaacgaatct cgaagtgccc acaaaatcga
     4981 gtgaaatgac ggccagtacg ccgtccacag ctacgccgcc tgctgacgcg acgttgcccg
     5041 gcattgcagc aggcgcgaag cccgatccgg tcactgttgc ggtagcacag tcggcgtcga
     5101 cttcaagtgt gacgacacca ggaggcggaa ccattcaaat tttgtcgggc cccaacgccg
     5161 gtagggaact gtcgctcgct aagccgctca ccacactggg caagcccggt gttcaagttg
     5221 cggttatcgc aaaaaggcct caagggtatt tcattaccca tgtagaagga gcccagtttc
     5281 cggtgctcaa cggtaaaacg ctcgactctc aggcacatca gcttggcgat cacgacatca
     5341 tcgagttagc cagtgtgaaa atggagtttt tcctgaagcc ctagagttgt tggcgccctt
     5401 actcgtatga gccgctaggg ccagtcagag ctgcggcgcg cgatctgcgc aaaattggac
     5461 aagagaggtg gcttgaagaa acacttggtg cgcatcgcgc tcggccttgt ggtggtcctt
     5521 ttctttgccg gccatgctgc gcggtattac gccatcccgt tcatcgatcg ccttgagaac
     5581 attgtttatg acgctcgttt gcggctgaca atggcgaaca gtgtggatcc gcggatcgta
     5641 atagtcgata ttgatgagaa aagccttgcc gttgagggcc gatggccttg gcgacgcgac
     5701 aaattggccg cgatgcttga tcaacttttc gatcgttaca aagtcagcat agtgggcttc
     5761 gatatcgttt ttgcagagcg ggacgagagt tccggtctcg gcatcttgcg ggagctcggg
     5821 caaagggagt tgaagggtga tgctcacttt caggcggtgc tcaatgctgt gggtccgaag
     5881 ttggaattcg atcgcgtttt tgcagaaaag ctcaagaatc gcgcaatcgt ccttggatac
     5941 tatttttcca gtgttaagtc ggcggatgga aaagggcgat ccgccggcgc tcttcccaag
     6001 cccgttcttc cagcgggcac atttaaaggc aaaaatattc agttcacaaa ttgggttggc
     6061 tatggggcca atctggaaga atttcagcag gccgcccgca gcgcgggaca cttcaatcct
     6121 tggcctgacg aggatggcgt gatacgccgc gttcccatac tcgctgaatt cgacggcgct
     6181 tactacgaat ccctatcgat ggcagtcgtc cgcctggcac tgggattacc gccggtttta
     6241 cccggctttc cggaagggaa atccggatcc aaaaattatc cggggctcga atggattgat
     6301 ctcggaccgt tgcgtattcc ggtcgataat tccgttacgg cactcgttcc ctatcgaggc
     6361 aaggaaggca gtttcaagta tgtttcagcg accgatgtgc tgcatggcag tacgccgctg
     6421 gatgaactga agaacaaaat catgctgatt ggcacaacag ctccaggctt gtacgacctt
     6481 cgtacggcgc cggttgcaag cgtatatcct ggggtggaaa ttcatgcgaa cttaatagcc
     6541 gggatgctcg atggcagtat caaacaacgg cctccgtatg tattgggcgc cgaagtattg
     6601 ctgttattgt tttccggagt agctatggcg ctcgtcctgc cgttgctcaa tccattgcgc
     6661 gctaccttat tgacaatcgt ggtggtcgct gcagtattcg cggcaaatgt tctgatctgg
     6721 acggaaggaa atcttgtgct accccttgcc tccggattgt tgatgctggt catgctgttt
     6781 gcgctaaaca tgtcgtatgg tttttttgtg gaatcccggg caaaacggca gataaccggc
     6841 ctgtttggtc agtatgttcc tcccgaactg gttgacgaga tgagtaaaga tcccgaagcg
     6901 ttctcaatgg aagctgagag ccgggaactg tcagttctct ttactgacgt gcgaggcttc
     6961 accaccatct ccgagggctt ggaccccaag gaattgtcaa ggctgatgaa tgaatttctt
     7021 acgcctttaa cgcggatcat ctataagcac cgcggaacga tcgacaagta catgggcgat
     7081 tgcatcatgg ctttttgggg tgccccgctc gatgatccgc agcacgctcg caatgccgtg
     7141 ttggctggtc tggaaatgca tcgggtgctg gccggtctcc agccgcattt caaacaaaaa
     7201 ggtttgccat cgatccagat tggcgtgggt gtgaacagcg gtcgtatgag cgttggcaac
     7261 atggggtctg aaattcgttt ggcatatacc gtcatgggag atgcggtgaa cctggcgtca
     7321 cgactggagg gaatcaccaa gcagtatgga atcaagatga ttgtaggaga agctactcgt
     7381 gccgaattgc ccgacctgct gtttcgcgaa ctcgacagag taaaagtcaa gggtaaggaa
     7441 acatccgtgg caatctacga gccagttggt ttgccagtcg aaattgaaaa accgctgcag
     7501 gatgagttga aactctgggg tcaggcgctc aagctttacc ggatacgcga ttgggatatg
     7561 gcagaattac aactgatcaa cttaacgaag atgagtccgg gtagtgcact ttacgcgttg
     7621 ttcctcgacc gtattgcgca tctgcgcgtc aaccctcccg aagaaggctg ggacggttcg
     7681 tggacatttg agaaaaaata gcgatgcaga tcagaatact tggttgtagt ggcggcattg
     7741 gcggaaatct gcgcacaacc tcaatgctca ttgacaacga tgtactggtt gatgcaggca
     7801 ccggggtggg agatcttgcc attaccgacc tgctgaagat agatcacgtc ttcctgacgc
     7861 actctcatct cgatcacatt acttcattgc catttcttgt cgacacgatc gggccaatgc
     7921 gggaaacgcc aatcaccgta cattgcaccg aagctacgcg caaaattctc caggaacaca
     7981 ttttcaattg gaaagtctgg ccggatttta ctgaaatacc gagcaagtca tctccttgtc
     8041 tgtgctattc aacatttgct gttggagata tcgtcggact tggcggccgc aagattagag
     8101 tgctgcctgc aaatcacgta gtgcctgccg tggggtttca gttcgacagc ggtgaagcga
     8161 gcctcgtatt taccggagat accacgataa acgatgcttt gtgggatgaa gtaaacaaga
     8221 tttcaaacct tcgctatttg gttattgaaa ccgcgttccc gaatatggaa agggaattag
     8281 cgatcgcatc caaacacctc tgtcccagca tgctcgcgga agagttggca aagttaagac
     8341 gaccgacaag agtatttatt acgcatctca aaccgggggc cgggactctg acgatgcagg
     8401 aggta
//
LOCUS       DATMZQ010000440         9253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_880016, whole genome shotgun sequence.
ACCESSION   DATMZQ010000440 DATMZQ010000000
VERSION     DATMZQ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880016"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN496_14050"
     CDS             <1..275
                     /locus_tag="VN496_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXF18128.1"
                     /translation="YLERIDGAQKGSSRIAEEKGRAAILLKRPTKAIEDNIAEGRIVM
                     MGLPGAVPLEGGVPIVKDGQFLGAIGVSGVQSFQDGVIARAGVAAL"
     gene            complement(337..1539)
                     /locus_tag="VN496_14055"
     CDS             complement(337..1539)
                     /locus_tag="VN496_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18129.1"
                     /translation="MPRAPSLIIALATMIAVHGAQAEKHYGPGVSDSEIKIGQTMPYS
                     GPLSAFGTLGRAEAAYFDMVNAQGGVNGRKIKLLSLDDGYSPPKTIEQTRKLVESDEV
                     LLLFSSFGTPPNTAAMKYLNAKQVPQLFIAATGMKWSDPHNFPWTMAFLPSQKSGTAG
                     YVRYLLKVRPDAKIGVLYQNDDFGKDYVKALRDHLRDKAERMIVAEASYESTDPSVDS
                     QIATLKGAGADTFFSFTSPKFSAQAIRKAHDIGWKPLYFIPYSATSVSAVLQPAGLEK
                     SVGVISSAYVKDPTDPQWKADPATRDWLAWMKKYFADGDLAEIYNVYAYTNAQLLVQV
                     LKRCGDDLSRENIMKQAASLQNLELPMLLPGVRINTSATDYEPVKQLQLMRFDGKAWV
                     RFGEITSP"
     gene            complement(1589..2788)
                     /locus_tag="VN496_14060"
     CDS             complement(1589..2788)
                     /locus_tag="VN496_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18130.1"
                     /translation="MAIVNRVLLAFVVAMVAQSGHAEKKYGPGASDGEIRIGNTNPYS
                     GPASAYGTIGRAESAYFRMINDQGGINGRKINFISLDDGYSPPRTVEQVRKLVEQEQV
                     LFLAGTLGTPPNSAIHKYVNVNGVPHIFVNTGATKWGDPKNFPWTMGFNLNYQAEGHI
                     YAQYILDNKPDAKIAILFQNDDYGKDYVRGLKDGLGDKAKMVVAEASYEVTDPTIDSQ
                     IVTLKASGADTFYNVTTPKFAAQAIRKVYDMGWKPLHILNNVSNSVGSVLTQAGLEKS
                     VGVVSATYFKDPVDPQWQKDAEFKSYSDWFSKYYPEGDIKDVFNVAGYLIAQGVVHVL
                     KACGDDLSRENVMKQMASIRDLQMPMMLPGVKWNTSATDYYLIESAQLARFDGKEWVR
                     FGKIIGR"
     gene            complement(2816..4021)
                     /locus_tag="VN496_14065"
     CDS             complement(2816..4021)
                     /locus_tag="VN496_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18131.1"
                     /translation="MNLIHRVLVAALVVLAMPQLSHAQKKYGPGVTDTEIKLGQTMPY
                     SGPASAYGAIGKAELAYFEMINEQGGINGRKVNLLSLDDGYSPPRAVEQVRKLVEEDQ
                     VLALFQTLGTPSNSAIHKYVNAKKVPHLFIATGATKWGDPKNFPWTIGFQPSYQIEAK
                     IYATYILKNKPDAKIAVLYQNDDYGKDYVKGLKDGLGDKTGMIVAEASYEVSDPTVDS
                     QIVTLKSSGADVMFTIATPKFAAQAIRKVHDMGWKPLHFVNNVASSVGAVLSPAGLDK
                     SVGLITALYMKDPTDPQWEKDAGMRKWREFMKKYMPGADQADFNYVYGYGAAQLLTMV
                     LKACGDDLTRENLMKQATNLQDVTLDVTLPGIKYNTGPNDYFLVEQLQLARFDGKRWV
                     LFGEVLGHQ"
     gene            complement(4100..5302)
                     /locus_tag="VN496_14070"
     CDS             complement(4100..5302)
                     /locus_tag="VN496_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18132.1"
                     /translation="MQKSRLWLVACLAFLSASTATAEKKYDPGATDNEIKIGQTMPYS
                     GPASAYGTIGRAEAAYFKMINEQGGINGRKINFISLDDGYSPPKTVEQTRRLVEEEQV
                     LLIFQSLGTAMNSAIQKYMNAKNVPQLFVATGATKWGDPEHFPWTMGWQPNYQTETHI
                     YAKYLLQNKRDAKIAVLFQNDDYGKDYLKGLHDGLGQQTEKLIVREASYEATDPTVDS
                     QIVTLQGSGANVLFIFATPKFAAQAIRKTYDIGWKPMTFVNNVSTSVATVLQPAGLEK
                     STGLITALYFKDPTDPQWANDPSLKLWHDWMKKYYPEGDIKDGSNIYAYAAAQTMAHV
                     LKQCGDDLSHENIMRQAARLKDLEIPVLLPGIRINTGPADFYPIEQVQLARFDGKRWV
                     LFGEVIGK"
     gene            complement(5337..6359)
                     /locus_tag="VN496_14075"
     CDS             complement(5337..6359)
                     /locus_tag="VN496_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF18133.1"
                     /translation="MSTLSTKATTADFVASRFAGMSLRAWKYLGFAMLLAGAVALPFL
                     VSNYRVFQFNLVLIYAIVLLGLNILTGYNGQISLGHGAFYAIGAYSAAILMDHFSWPY
                     WATLPVAGLVCFCFGFLFGLPALRLRGHYLALATFALAVAMPQILKYKHIEQWTGGVQ
                     GIVIMKPAAPFGLKITPDQWLYLFTLAILLLAFAAGWNLLRGRVGRALVAIRDHPVAA
                     EAMGVHSALYKTLAFGVSALFTGVAGALGAIVVQFVAPDSFAAGLSINFLIGIVIGGV
                     ASISGAFYGALFIQFVPNFADQISKAAPWAIYGVFLLAFVYVMPTGVAGFVRLAWNRI
                     RRASGR"
     gene            complement(6399..7271)
                     /locus_tag="VN496_14080"
     CDS             complement(6399..7271)
                     /locus_tag="VN496_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF18134.1"
                     /translation="MEAFLHQVFSGLASGGIYASLALALVMIYQSTNHINFAQGEMAM
                     FSTYLAWSMINSGMPYWWAFLLTVLISFVTGIVIERIIIRPVEHAPVLSIITVFVALL
                     VIFNSVAGWIFTYTIKTFPSPFPQQPLFGNRYISSHELGSIGITLLVLVALFLFFRFT
                     PLGLAMRAVAQNPQSSRLVGIRVGWMLALGWGLAAAVGAVAGMLVAPIVYLEPNMMSG
                     VLLYAFAAALVGGIDNPFGAVVGGFMVGVLENLIGAYVIGTELKLTVALVLIVGVLLF
                     KPSGMFGKVFVSRV"
     gene            complement(7290..8000)
                     /locus_tag="VN496_14085"
     CDS             complement(7290..8000)
                     /locus_tag="VN496_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18135.1"
                     /translation="MPLLEVTNLAAGYNIADVLRGVSFAVEQGGITAVLGANGAGKTT
                     LLRAVSGMLRRRGSIRFDGRPIETMATEDIARLGVAHVPDGRGTFLDLTTEENLRIGA
                     YTRKDRAAVARDLERVFTYFPRLKERRYQQAGTLSGGEQQMLAVSRALMLRPRLLLLD
                     EPSFGLAPLVVQDIFSIMRAINAEEGVSVLLVEQNAGLALDLADNAYVLETGQVVMSG
                     TTDAVRSDEAVRRAYLGY"
     gene            complement(8000..8830)
                     /locus_tag="VN496_14090"
     CDS             complement(8000..8830)
                     /locus_tag="VN496_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18136.1"
                     /translation="MERSLSAQEQTDPARREVAASAEALLRVTNITVRFGGIVALEDV
                     SFDVMPGQIAGVIGPNGAGKTTLFNCLSRLYPFQAGGILFQNRSLLEVPRHRIAEFGI
                     GRTFQNLALFRTMNVLQNIMVGAHSWSRGGFLANALRLPIVAREEAHVANEAGELLRL
                     LELESVAHNRVADLPFGTQKRVELGRALASRPKLLLLDEPAGGLNHEEVASLSALIRR
                     IRDQLGVTVLLVEHHMGLVMQLSDKVVALNFGKKIAEGLPADVQRNADVIRAYLGKTA
                     "
     gene            9028..>9253
                     /locus_tag="VN496_14095"
     CDS             9028..>9253
                     /locus_tag="VN496_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18137.1"
                     /translation="MSNIAGWQKAFTHNDDLASGAEGRPLGERPVQPDHYQFWPAHLP
                     REMPLPETSLYRNLEATAARFPDKPVVQYYG"
BASE COUNT         1988 a         2746 c         2514 g         2005 t
ORIGIN      
        1 tgtacctcga acggatcgac ggcgctcaga aaggctcgag ccggattgcc gaggaaaaag
       61 gccgagccgc gattctgcta aagcggccga ccaaagccat cgaggacaat attgcagaag
      121 gtcgaatcgt catgatggga ctgcccggtg cagtaccact ggaaggcggc gtgccaatcg
      181 tgaaggatgg ccagttcctc ggcgcgatcg gcgtgtctgg ggtgcagtcg tttcaggatg
      241 gcgtcattgc acgcgcaggc gtggccgcgc tttaaccggc tcagcggctg atgtaatgcg
      301 actgattgtc ggcgtaatcg acgctatccg cgaccatcag gggctggtta tctctccgaa
      361 ccttacccat gctttgccgt cgaagcgcat cagctgaagt tgctttaccg gctcgtaatc
      421 ggtagcgctc gtattgatcc tgacccctgg caacagcatc ggcagttcca gattttgcag
      481 gctggcggcc tgcttcatga tgttttcgcg tgaaagatcg tcgccgcatc gttttagcac
      541 ctgtaccagc aactgtgcat tggtgtaggc atagacgtta taaatttcgg cgagatcgcc
      601 gtcagcgaaa tactttttca tccaggccag ccaatcccgc gtcgccgggt cagccttcca
      661 ctgcggatca gtcggatctt tgacgtaggc ggacgaaatc acgcccaccg atttctccag
      721 tccggccggc tgcagcacgg cgctgaccga ggtcgcgctg tacggaatga aataaagcgg
      781 tttccaaccg atgtcgtgcg ccttgcgtat ggcctgcgcc gagaatttcg gtgaagtgaa
      841 actgaagaac gtgtctgcac cggcgccttt caacgttgcg atctgggaat ccaccgatgg
      901 atcggtcgat tcgtatgatg cttccgcgac aatcatgcgc tccgccttgt cgcgcaggtg
      961 atcgcgcaac gccttgacat aatccttgcc gaaatcgtca ttctggtaga gcacgccgat
     1021 ttttgcgtcc ggccgcactt tcagcagata gcggacatag cccgctgtgc cggatttctg
     1081 gctcggcaga aaggccatgg tccacggaaa attatgcggg tcgctccact tcatgccggt
     1141 ggcggcgatg aataattgcg gcacctgctt tgcattgaga tatttcatgg ccgcagtgtt
     1201 cggcggcgtc ccgaacgaac taaacaacag cagcacctcg tccgactcga ccagcttgcg
     1261 cgtctgttcg atggttttcg gtggactgta gccgtcgtcg agcgaaagaa gcttgatctt
     1321 gcgcccgttg actcctcctt gcgcgttgac catgtcgaag taggcagctt cagcgcggcc
     1381 cagggtaccg aacgcagaca acgggccgct gtagggcatg gtctgcccga tcttgatctc
     1441 gctgtctgac acacccggtc cgtaatgttt ttctgcctgg gcaccatgca ccgcgatcat
     1501 cgtcgcgagc gcaataatga gactcggagc gcgaggcata gacgctgcgc cggcatccga
     1561 ccttttgcct ggtgtaccag gcggcatgct aacgcccgat aatcttgcca aagcgcaccc
     1621 actccttgcc gtcgaagcgc gcaagctgcg cggattcgat caggtaataa tccgtagcac
     1681 tggtgttcca cttgacgccc ggcaacatca taggcatctg cagatccctg atcgaggcca
     1741 tctgtttcat gacattctcg cggctcaaat catcgccaca ggccttgaga acgtgcacca
     1801 cgccctgggc gatcagataa ccggcaacgt taaagacatc cttgatatct ccttccggat
     1861 aatacttact gaaccagtcg gagtaagact tgaactcggc gtctttttgc cattgcggat
     1921 ccaccgggtc cttgaagtaa gtggcgctga ccactcccac cgatttttcc agacctgcct
     1981 gtgtgagcac ggaacccacc gaattcgaca cattgttgag aatgtgcagc ggtttccatc
     2041 ccatgtcgta gactttgcgg atcgcctggg ccgcaaactt tggcgtggtc acgttgtaga
     2101 acgtgtccgc gcccgacgcc ttgagggtga cgatctgcga gtcgatggtc ggatcggtga
     2161 cctcgtagct cgcctcggcg acgaccattt tcgccttgtc acccagtccg tccttcagcc
     2221 ccctgacgta gtccttgcca tagtcgtcat tctggaaaag gatcgctatc ttcgcgtcag
     2281 gcttgttatc gaggatgtat tgcgcgtaga tgtgaccctc ggcctgataa ttgagattga
     2341 agcccatggt ccagggaaaa ttcttcggat cgccccattt ggtggcgccg gtgttgacaa
     2401 agatgtgtgg caccccgttg acattgacgt acttgtggat cgcggaattg ggcggcgtac
     2461 ccagagtgcc cgccagaaac agcacttgtt cctgttcgac cagtttgcgc acctgttcca
     2521 cggtgcgtgg cgggctgtaa ccgtcatcga gactgatgaa attaatcttg cggccattga
     2581 ttccgccctg gtcattgatc atcctgaaat aggcgctctc ggcgcgtccg atcgtgccgt
     2641 aggcagaggc tgggccgcta tagggattag tgttgccaat ccgtatttcg ccgtcgcttg
     2701 ccccaggacc atatttcttt tcggcatgac ctgactgcgc aaccatcgcc acaacaaaag
     2761 cgagcagtac acgattgaca attgccatgt tcatctcccc gtcgtagtga cctatctatt
     2821 ggtggccgag gacttcccca aatagcaccc agcgcttgcc gtcaaagcgc gccaactgca
     2881 actgctccac cagaaaataa tcgttcgggc cggtgttgta tttgatcccg ggaagagtca
     2941 cgtccagcgt gacgtcttgc agattggtcg cctgcttcat caggttttcg cgagtcaggt
     3001 catcgccgca ggctttcagt accatcgtca agagttgggc agcgccatat ccgtaaacgt
     3061 agttgaagtc cgcctggtcc gctccgggca tgtacttttt catgaactca cgccacttcc
     3121 gcataccggc gtctttctcc cactgcggat ccgtgggatc tttcatatag agcgccgtga
     3181 tcaaccctac cgacttgtcc agacccgcgg gcgaaagcac cgcccctacc gacgaagcga
     3241 cattattgac gaagtgcaag ggcttccagc ccatgtcgtg gaccttgcga atcgcctgtg
     3301 ccgcgaactt gggcgtcgcg atggtaaaca tcacgtccgc gccggacgat ttcagcgtca
     3361 cgatctggga atccaccgtg ggatcgctga cttcatagct tgcctcggcc acgatcatcc
     3421 ctgttttgtc acccaagcca tctttcaatc ccttcacgta atccttgccg taatcatcgt
     3481 tctgatacag gaccgcaatt ttggcgtcag gcttgttctt cagaatgtac gtagcgtaga
     3541 tctttgcttc gatctgataa ctgggctgga agccgattgt ccagggaaaa tttttcgggt
     3601 ccccccactt cgttgcaccc gttgcaatga acagatgggg cacttttttg gcattgacgt
     3661 atttgtggat ggctgaattg gaaggcgttc ccaaggtctg gaacagcgcc agcacttgat
     3721 cttcctcgac cagcttgcgt acctgctcga ccgcccgggg tggcgaataa ccgtcatcca
     3781 ggctaagcag gttcaccttg cgcccattga ttccgccctg ctcgttgatc atctcgaaat
     3841 aagccaattc cgccttgcca attgcgccgt aagccgaggc agggccgctg taaggcatgg
     3901 tttgcccgag cttgatttcg gtgtccgtta cacccgggcc atattttttt tgcgcatggc
     3961 tcaactgcgg catggccagc acaaccaggg ccgcaaccaa cactcgatgg atcaggttca
     4021 ttcgttttcc ttatctagat taattcagtg acaagccgac tgcttatcgc ggcattatcg
     4081 cttctgcgtc gacggcaatc tacttgccga taacctcgcc aaacaacacc catcgtttgc
     4141 catcaaaccg cgccagctgt acctgctcga tcggatagaa gtccgcgggg ccggtgttga
     4201 tcctgatccc tggcaggagc accggaattt ccaaatcctt caaccgcgcc gcttgtcgca
     4261 tgatgttttc gtgggaaagg tcatcaccgc attgcttgag aacatgagcc atggtttgcg
     4321 cagccgcata ggcgtatatg ttggagccgt ctttgatgtc accttccgga taatatttct
     4381 tcatccagtc gtgccaaagc ttcagggacg gatcgtttgc ccactgggga tcggttggat
     4441 ccttgaagta cagcgccgtt atcagcccgg tcgatttttc cagtccagcg ggctgcagga
     4501 cagtagccac cgacgtcgat acattgttga cgaaggtcat cggcttccag ccgatgtcgt
     4561 aagtcttgcg gatcgcctgg gcagcaaact ttggggtggc aaatatgaaa agcacgttgg
     4621 cacccgaacc ctgcagggtg acgatctgcg agtccaccgt cgggtccgtg gcttcgtagc
     4681 ttgcttccct gacgatcaat ttctcggtct gttggcccag cccgtcgtgc aggcctttca
     4741 ggtaatcctt gccatagtca tcgttctgga acagcactgc gattttcgcg tcgcgtttgt
     4801 tctgcagcag atattttgcg tagatatggg tctcggtctg gtagttcggt tgccagccca
     4861 tggtccacgg aaagtgctcc ggatctcccc atttggtggc gccggtggcg acgaacagtt
     4921 gcggcacgtt cttggcgttc atgtacttct ggatcgccga gttcattgcc gtccccagac
     4981 tttgaaaaat cagcaatacc tgttcttcct ccacgagcct gcgcgtctgc tccaccgttt
     5041 ttggcgggct gtacccatcg tcgagcgaaa tgaagttgat ttttcggcca ttgatcccgc
     5101 cttgctcgtt aatcattttg aaataggcag cctctgctct gccaatcgtg ccgtaagcgg
     5161 atgcaggacc gctgtagggc atcgtttggc cgatttttat ttcattgtcg gtcgcgcctg
     5221 gatcatattt tttctccgcc gtcgcggtcg acgcagatag aaacgccaaa catgctacta
     5281 accaaagccg gcttttctgc atgacgtcct ccgctcctgg taaatggcct gatttgttat
     5341 cttccgctgg cgcgccttat ccggttccat gccaaccgca caaacccggc gactccggtt
     5401 ggcatcacat agacaaacgc cagcaggaac acaccataga tcgcccacgg agccgctttc
     5461 gagatctggt cggcgaagtt cggtacgaac tggataaaca atgcgccata gaatgctccg
     5521 gagatcgatg cgacgccacc gatcacgatg ccaatcagaa aattgatcga taagcctgcc
     5581 gcaaaactgt caggtgccac gaactggacc acgattgccc ccagcgcgcc cgcgacgccc
     5641 gtgaacagcg cactcacgcc aaaggcgagc gtcttgtaga gcgcgctgtg aacacccatc
     5701 gcctccgccg cgacaggatg atctcgtatg gccaccagtg cgcgtccgac gcgtccccgc
     5761 aacagattcc agcccgccgc gaatgccagt agcaaaatcg ccagcgtgaa caggtaaagc
     5821 cactgatctg gcgttatctt caacccgaaa ggcgcggccg gcttcatgat cacaatgccc
     5881 tgtacgccac cggtccattg ctcgatgtgt ttgtatttga ggatctgcgg cattgccact
     5941 gccagcgcaa aagtcgccag cgccaggtaa tgtccgcgca agcgcagtgc cggtaaccca
     6001 aacagaaagc cgaagcaaaa gcacaccagc cccgctaccg gcaaagtggc ccagtacggc
     6061 caactgaagt ggtccatgag gattgcggcc gaatacgcgc cgatggcgta aaacgcgccg
     6121 tgccccaggg agatctgacc gttgtagccg gtcaggatat tcagacccag caaaacgatc
     6181 gcgtagatga gtacgaggtt gaactggaat acccggtagt tgctgaccag gaaaggcagg
     6241 gcaactgccc cggcgagtag catcgcaaag ccgaggtact tccaggcgcg tagcgacatc
     6301 cccgcaaagc gggaggcgac aaaatcggct gtggttgctt tcgtgctcaa ggtgctcata
     6361 aggcgcagac catgtgatca gcaacaagtc gtcgagcatt acacacggga aacgaatact
     6421 ttgccgaaca tgccgctcgg cttgaacagc agcacgccga caatcaaaac cagcgcgacc
     6481 gtgagcttca gttcggtgcc gatgacgtaa gcaccgatca aattctccag caccccgacc
     6541 atgaatccgc ccaccaccgc gccgaacgga ttgtcgatgc cgcccaccaa tgcggccgcg
     6601 aatgcatata acagaacgcc ggacatcatg ttaggttcca aatagacgat gggtgccacc
     6661 agcatgccgg cgactgcacc gaccgcggca gccagccccc atcccagggc caacatccat
     6721 cccacgcgaa tgccaaccag ccggctggac tgcgggtttt gcgcgaccgc acgcatcgcc
     6781 agtcccagtg gcgtgaagcg aaaaaacagg aacagcgcca cgagaaccag cagcgtgatg
     6841 ccgatcgaac ccagttcatg cgaggaaatg tagcggttgc cgaatagcgg ttgctgcgga
     6901 aaaggacttg ggaatgtttt gatcgtgtag gtgaatatcc agcccgccac actgttgaag
     6961 ataaccagaa gcgcaacaaa tacggtaatg atggaaagta ccggtgcatg ctccacgggc
     7021 cggataatga tgcgctcaat gacaatgccg gttacaaatg aaatcagtac ggttagtaaa
     7081 aaggcccacc aatacggcat gccgctgttg atcatgctcc acgccaggta agtcgagaac
     7141 atggccattt cgccctgggc gaaattaatg tgattggtcg actggtagat catgaccaat
     7201 gccaaagcga gactggcata tattccacca ctggccagac cggaaaacac ctgatgcagg
     7261 aacgcttcca tggcttctct tagaacggtt cagtaaccca ggtaggcgcg ccgcaccgct
     7321 tcgtcggagc gcaccgcatc ggtcgtgcct gacatgacga cttgtccagt ctccagcaca
     7381 tacgcgttat cggccagatc cagggccagc cctgcgttct gctcgaccag cagaacgctc
     7441 acgccttcct cggcattgat ggcgcgcatg atcgaaaaaa tgtcctggac caccagcggc
     7501 gccaaaccga atgacggctc gtccagcaac agcaagcgcg ggcgcaacat caaggcgcgc
     7561 gacaccgcca gcatttgctg ttcgcctccg gaaagcgtgc cggcctgctg gtagcggcgc
     7621 tctttcaagc gcgggaaata cgtaaacacg cgttccaggt cgcgggctac tgcagcgcga
     7681 tctttgcgcg tatacgcgcc gatgcgcaga ttttcttccg tggtcagatc gaggaacgtt
     7741 cctcgtccgt ctggaacatg cgccacgccg agacgggcaa tgtcttccgt ggccatggtt
     7801 tcaatagggc ggccgtcgaa gcgaatggag ccgcgccgcc tcaacatgcc gctgaccgca
     7861 cgcagcaaag tcgttttgcc agccccgttt gcccccagca ctgcggtgat gcctccttgc
     7921 tccacggcaa agctcacgcc gcgcaaaaca tccgctatgt tgtagccggc agccaggttg
     7981 gtgacttcga gtagcggcat caggctgttt tcccgaggta ggcgcgaata acatcggcgt
     8041 tgcgctgcac atcggcgggc aagccctcgg ctatcttttt gccgaaattg agcgcaacca
     8101 ccttgtcgga aagctgcatg accagaccca tgtggtgctc aaccagcaat accgtgacgc
     8161 caagctgatc ccggatgcgt cgaatcagtg cggacagcga cgccacttct tcgtggttca
     8221 ggccgccagc tggctcatcg agcaacagca acttcggccg gctcgccaat gcacggccaa
     8281 gttccacgcg cttttgcgta ccgaaaggca gatccgccac ccggttgtgc gccacggatt
     8341 cgagttccag caatctgagc agctcaccag cctcattcgc gacatgcgcc tcttcgcgcg
     8401 cgacgattgg caggcgcaac gcatttgcca gaaaaccgcc gcggctccaa ctgtgtgcgc
     8461 cgaccatgat gttttgcaga acattcatgg tccggaacag tgcgaggttc tggaaagtcc
     8521 gtccaatgcc gaattcagcg atgcggtggc gcggcacctc gagcagcgag cggttctgga
     8581 acagaatgcc gccggcctga aacggataca gacgcgacag acagttgaac aacgtggttt
     8641 ttccagcgcc gttgggacct atcacgccgg caatctggcc cggcatcaca tcgaaggaca
     8701 cgtcctccag tgccacgatg ccaccaaagc gcaccgtgat attagtcacg cgcagtaatg
     8761 cctctgcgct cgcggcaact tcgcgccggg cagggtcggt ttgctcttgt gcgctcaaac
     8821 tgcgttccaa cattcctccc agatttatgg atcgcgccga tcgaactccc cggcgatact
     8881 ggcataactt gtaggcgccg gtaaagttcg atgcgttcgc tgcagcgcaa tgcgaaccag
     8941 cgttcactgc gtcgaacatg gttcgcactc cgggcagcaa atattgcact tcacattcga
     9001 cagcacgcat tgacctgagc ggcgggcatg tccaacatag ccggctggca aaaggcattc
     9061 acacataacg acgacctggc cagtggcgcc gaaggcaggc cactaggaga gagacccgtg
     9121 caacccgatc attaccaatt ctggcccgct catttgccga gggaaatgcc gttgccggaa
     9181 acaagtctgt atcgaaacct cgaggccacc gcggcgcgct tcccggacaa acccgtggtg
     9241 cagtactacg gaa
//
LOCUS       DATMZQ010000441         2021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4686412, whole genome shotgun sequence.
ACCESSION   DATMZQ010000441 DATMZQ010000000
VERSION     DATMZQ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4686412"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="ubiB"
                     /locus_tag="VN496_14100"
     CDS             complement(<1..1216)
                     /gene="ubiB"
                     /locus_tag="VN496_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HXF18138.1"
                     /translation="MHLLRLFKILRVSLRYGLDEFILGHERVRMLRPLLSAALFWRHL
                     GRPRGERLRLALEALGPIFVKFGQVLSTRRDLIPLDIADELAKLQDQVPPFPVEQVYR
                     TIERTYRLPVEQVFEHFDAVATASASVAQVHFAILPGGREVAVKVLRPGIARVIVEDV
                     ALLNVAASLMEKLWADGRRLKPREVVAEFERHLRDELDLLREAANASQLRRNFDNSSL
                     LLVPEVFWDFCGTDVMVMERMSGTPISQVERLRKQGVDVPKLARSGVEIFFTQVFRDG
                     FFHADMHPGNILVAADGRYIALDFGIMGTLNEVDKNYLAQNFLAFFRRDYRSVAQIHL
                     ESGWIPADTRIEEFETAIRAVCEPIFDKPLKEISFGKVLLRLFQTSRRFNIEVQPQLV
                     LLQKTLLNIEGLG"
     gene            complement(1229..1834)
                     /locus_tag="VN496_14105"
     CDS             complement(1229..1834)
                     /locus_tag="VN496_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol-binding protein"
                     /protein_id="HXF18139.1"
                     /translation="MSAINSETMLSRPFSAFVNHLLGSAEWARGSLAPHAGKIAVFDI
                     FPVRVAVAVDAEGMLRPVEAGVAPSVTIRLTPAIALKIMAEGGAAWRNAEVDGDTEFA
                     SALSRIAANIDWDFEEDLSRVFGDIAAHRLATAARAAAAWPKQATTSIATGLAEYLSE
                     EKHLLVTPLRAGEFVRDVDELRDAVERLDKRIEHLQRRADG"
     gene            complement(1815..>2021)
                     /locus_tag="VN496_14110"
     CDS             complement(1815..>2021)
                     /locus_tag="VN496_14110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF18140.1"
                     /translation="DAYSFRVLPLLGKLVAGDADSYRYLVESIRKHPGQEELKQLMQQ
                     AGCSRVDYYNLTAGVVALHVGHKF"
BASE COUNT          431 a          636 c          557 g          397 t
ORIGIN      
        1 ggccgaggcc ttcaatgttc aatagcgttt tctgcagcaa aacgagctgc ggttgcactt
       61 cgatgttgaa cctccgcgac gtctggaaca ggcgcagcaa tactttgcca aacgatattt
      121 ccttgagtgg tttgtcgaag atcggttcgc acactgcgcg gatcgccgtt tcaaattcct
      181 caatccgcgt atccgctgga atccatccgg actccagatg gatttgtgcg acgctgcggt
      241 aatcacgccg gaagaaggcc agaaaattct gcgcgagata gtttttgtcg acttcgttca
      301 atgtgcccat gatgccgaag tcgagtgcga tgtagcgacc atctgctgcc acgagaatgt
      361 ttccgggatg catatcggca tggaaaaacc cgtcgcggaa tacttgcgta aagaatatct
      421 ccacaccgga ccgcgccagc ttcggcacat cgacgccttg cttgcgcaag cgttccacct
      481 ggctgatggg cgtacccgac atgcgctcca tgaccatgac atccgtgccg caaaagtccc
      541 agaacacctc tggaacgagc agcagcgatg aattgtcgaa attgcgccgg agctggctgg
      601 cattggctgc ctcgcgcagc aaatccaatt cgtcccgcag atgcctttcg aactctgcca
      661 cgacttctct cggcttgagt cgccgcccat cagcccagag cttttccatc aggctggctg
      721 caacgttcag caacgcaacg tcctcgacga tcacacgcgc gatgccggga cgcaacacct
      781 tgacggcaac ttcacgcccg cccggaagga ttgcaaagtg tacctgcgct acggaagcgc
      841 ttgccgttgc gaccgcgtcg aagtgctcga atacctgttc gaccggaagg cgataagtac
      901 gctcgatggt gcgatagacc tgctctacgg gaaacggggg aacctgatcc tgcaacttgg
      961 ccagctcgtc ggcgatgtcc agcggaatca aatcacgtcg cgtggacagt acctggccga
     1021 atttgacgaa tatgggcccg agcgcctcca gtgccagtcg cagccgctcg ccacgcgggc
     1081 gcccaagatg gcgccagaac agcgccgccg acaacaaggg acgcagcatg cgcacacgtt
     1141 catgaccgag gataaattcg tcgagtccgt agcgcagtga tacacgcagg attttgaaca
     1201 gcctcagcag gtgcattcgg cagaccgttc agccgtcggc acgccgctgc agatgctcga
     1261 tgcgcttgtc gagccgctct accgcatcgc gcagctcatc gacgtcccgc acgaattctc
     1321 ccgcacgcag gggcgtgacg agcaggtgtt tttcctcgct gaggtattcc gccaggccag
     1381 tcgcgatact ggttgttgcc tgcttcggcc aagccgcggc cgcgcgtgct gctgtggcca
     1441 ggcgatgcgc cgcaatgtcg ccgaacaccc ggctcaaatc ttcttcgaag tcccagtcaa
     1501 tattggcagc gatcctggac agcgccgatg caaactcggt gtcgccgtct acttctgcat
     1561 tgcgccaggc tgctccgccc tcggccataa tcttcagcgc gatcgccggt gtcaggcgaa
     1621 tagtcaccga cggggctacc cctgcttcga ccggacgcag catgccttcg gcatcaacgg
     1681 ccacggcaac acgcacggga aagatgtcaa acaccgcaat tttgcctgca tgcggcgcca
     1741 gactcccacg cgcccattcc gcactgccca gcaaatggtt tacgaatgcg ctgaacggcc
     1801 gggacaacat cgtctcagaa tttatggccg acatgcagcg ccacgacccc cgcagtcaga
     1861 ttgtaatagt caacgcggct gcagccagcc tgttgcatca gctgcttcag ctcttcctgg
     1921 cctggatgct tgcgaatgga ttcgacaaga taacggtagc tgtccgcgtc tccggcgacc
     1981 agcttgccaa gcagcggcag cacccgaaac gaataagcgt c
//
LOCUS       DATMZQ010000442         4046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3653272, whole genome shotgun sequence.
ACCESSION   DATMZQ010000442 DATMZQ010000000
VERSION     DATMZQ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3653272"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2309
                     /locus_tag="VN496_14115"
     CDS             <1..2309
                     /locus_tag="VN496_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXF18141.1"
                     /translation="ARVIAGRVARFHQSDLIKARLAFLEAKEAVLKKAVSTPPRPAYY
                     CSGCPHNTSTKVPEGSFALAGIGCHVMATAIYPEHTKTTCQMGGEGAAWIGQAPFSKV
                     PHVFANLGDGTYFHSGHLAIRAALAANVNITYKILYNDAVAMTGGQPVDGQISVAVIA
                     QQMAAEGVKRIAVVTEELSRYEDRSQLPANVTVNDRREMETVQRELRDIPGVSVLIYD
                     QTCAAEKRRRRKKGEYPKATQRVFINEAVCEGCGDCSVQSNCVSILPLETEFGRKRAI
                     DQSSCNQDYSCLKGFCPSFVTIEGGVPKKSRAAKQALPAELEALPEVSVPELGQAPYN
                     ILITGIGGTGVITIGALLGMAAHLESKGTSALDMTGMSQKNGAVTSHVRIARAPEDIR
                     AQRIATGEADLILGCDMLTAGAHDAIAKTRPGRTVAVINTHQQPPGQFTQNPDWQFPA
                     EQIRALLEESVSGRCHFIDATSLATALLGDSIATNLFMLGYAFQKGLIPVKEQSILKA
                     IELNGVAIDANMQAFTWGRRAAVNLQEVQTIATPAQPLIMQLPQNLETLIRRRVAFLS
                     DYQNRSYAKRYESLVTKVRQTESGLGSGDALSKAVARYFFKLMAYKDEYEVARLYTNG
                     QFDKRLSESFEGDFKVTYNLAPPLFAKRDAHGHLVKARYGSWMKTAFRLLAKLRGLRG
                     TAFDVFGYSEERKLERALIREYEALVTGMLPNLSPQNYRIAVELAALPERIRGFGHVK
                     QRHMADVNVERERLLQQFRNGNGVGLAA"
     gene            2407..4046
                     /locus_tag="VN496_14120"
                     /pseudo
     CDS             2407..4046
                     /locus_tag="VN496_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549638.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog.; frameshifted; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
BASE COUNT          907 a         1183 c         1180 g          776 t
ORIGIN      
        1 tcgcgcgcgt aatcgccggg cgtgttgcgc gctttcacca aagcgacctc attaaggcgc
       61 gcctggcttt tttggaggcc aaggaagcgg tattgaaaaa agcggtgagc acaccgccgc
      121 gtccggctta ttactgttcg ggttgtccgc acaatacctc caccaaggtg ccggaaggca
      181 gcttcgcatt ggccggaatc ggctgccacg tgatggcgac cgcgatctat cccgagcaca
      241 ccaaaaccac gtgtcagatg ggcggcgagg gagccgcctg gatcggccag gcaccctttt
      301 caaaagtgcc gcatgtattc gcgaacctgg gcgacgggac ttattttcat tccggccatc
      361 tcgcgatacg ggccgcgctc gcggccaacg tcaacatcac gtacaagatc ctctacaacg
      421 atgctgtcgc catgaccgga ggccagccgg tggatggcca gatttccgtt gcggtgatcg
      481 cgcaacagat ggctgcggaa ggcgtcaagc gcatcgccgt agtgacagaa gagttgtcgc
      541 gctacgagga ccggtcgcag ttgccggcga atgtcacggt caacgaccgc agggaaatgg
      601 aaaccgtgca gcgcgaattg cgcgacattc ctggtgtttc ggtattgatt tacgaccaga
      661 cttgcgctgc ggaaaagcgg cgtcgccgca aaaagggcga gtatcccaaa gccacccaac
      721 gcgtgttcat caatgaagcg gtctgcgaag gttgcgggga ttgcagcgtg caatcaaatt
      781 gcgtttcgat cctgcccctg gaaacggaat tcggacgtaa gcgggcgatc gatcagtcgt
      841 cctgtaatca ggactattcc tgcctgaaag ggttttgtcc gagcttcgtc accattgaag
      901 gcggtgtgcc gaaaaagtcg cgcgcggcaa agcaggcgct tcccgcagaa ttggaggcgc
      961 tgcccgaggt atccgtcccg gaacttgggc aggcaccgta taacatcctg atcactggta
     1021 tcggaggcac cggcgtcatt acgatcggcg ccctgctggg gatggcggcc cacctggaga
     1081 gcaagggcac gtctgcgctc gacatgactg gcatgtcgca gaagaacggc gcggtcacat
     1141 cgcacgtgcg catcgcgcgc gcacccgaag atattcgggc gcagcgcatc gcaacgggtg
     1201 aagcagatct tattttggga tgcgatatgc tgaccgctgg tgcgcacgat gccatcgcca
     1261 agacgcggcc cggtcgcacg gtagcggtca tcaataccca ccagcagccg cccgggcagt
     1321 tcacgcagaa tccagattgg cagtttccgg ccgaacagat tcgcgcgctg cttgaagaat
     1381 ccgtcagcgg ccgttgccat tttatcgacg ccacgtcgct cgcaactgcg ttattgggag
     1441 attcgatcgc gaccaatctg ttcatgttgg gttacgcatt tcagaagggg ctcatcccgg
     1501 tgaaggagca atccatcctt aaggcgatcg agctcaacgg cgtcgcaatc gacgccaata
     1561 tgcaggcatt tacctggggt cggcgcgctg ccgtcaatct gcaagaagtg cagaccatcg
     1621 ccactcctgc tcagccgctg atcatgcaac ttccacagaa tctggaaacc ttgatccgcc
     1681 gtcgcgtcgc tttcctgagc gattatcaaa accgttccta cgccaagcgt tacgagagtc
     1741 tggtcaccaa ggtacgccag accgaaagtg gtctggggtc cggagacgcc ttaagcaaag
     1801 cagtggccag atattttttc aagcttatgg catacaagga cgagtacgag gtcgcgcggc
     1861 tttacaccaa cgggcaattt gacaaacggc tcagcgaatc tttcgaaggt gatttcaagg
     1921 tcacttataa ccttgccccg ccgttgttcg ccaagcgcga cgcgcacggt catctcgtaa
     1981 aagctcgtta cggctcgtgg atgaagactg cattcaggct gctggcgaag ctcagaggcc
     2041 tgcgagggac tgcgtttgac gtgttcggct acagcgaaga aagaaagctg gagcgcgcac
     2101 tgattcgcga atacgaagcg ctcgtcaccg ggatgctacc caatctgtca cctcaaaact
     2161 atcggatagc ggtggaactt gcggcgctgc cagagcgcat tcgcggcttt ggccatgtca
     2221 aacagcgcca catggccgac gttaatgtag agcgcgagag gttgctgcag caattcagaa
     2281 atggcaacgg tgtcgggctg gctgcatgaa gagaggtaaa cccggccgat ttgcgttaac
     2341 gttcgaaaga taccaaacga ctaccctgga atatccgggg tggcgaaaga tcgggagccc
     2401 agaaccatga ccgacctaag cgagcagccg gcggaccaca aaacccgggc tccttacaag
     2461 atccgcttcg ttaccgcagc cagcctgttc gacggtcacg acgcctcgat caatatcatg
     2521 cgccgcatcc tgcaggccag cggcgtcgag gtcattcatc ttggccataa tcgctcggtc
     2581 gccgaaatcg tcaatgccgc aattcaggaa gacgtgcaag gcattgcgat ttcctcctat
     2641 cagggtgggc acgtcgagta tttcaaatac acgctcgacc tgctgcgcga acgcggcggt
     2701 gaagacatca gggtttttgg cggcggtggc ggcgtcattg ttcccgcgga aattgaagag
     2761 ttgcatcggt acggcgtagc ccgcatctac tctcctgacg acggcgcaaa aatgggactg
     2821 cagggcatga tcagcgacat gatccgcact gcggatcgca accttgccgc cgtcttgccg
     2881 gacagccttg gcgaactgtt caacggcaat tctcgcgcat tggcgcgcat cattaccgga
     2941 cttgaaaacg ccacactgcc ggagccgttg cgcaagcaac tcaccgcaca cgcggtccgg
     3001 aacgaccggg taccggtgct gggtatcacc ggcaccggcg gctccggcaa atcatcgctg
     3061 accgacgaac tgatccggcg tttcaggatc gatcaggccg acgctcttaa gatcgcggtg
     3121 attgcggtcg atccgtcgcg gcgtaaaaca ggtggtgctt tgctgggcga tcgcatacgc
     3181 atgaactcca tcaaccatgc caatatctac atgcgttcgc tggcgacacg cgacgctggc
     3241 agtgaaatag ccgcctgcct cccagaagtc gtggcggcct gcaaatgcgc cgggttcgat
     3301 cttgtgctgg tcgagacttc aggtatcgga cagggcgatg ccgcgattgt cccgcatgtc
     3361 gatttttcgc tgtgcgtcat gacgtacagc gaaaaatcga catgctggat ttcgcggact
     3421 ttgtcgccat caacaagttc gaccgaaggg gcgcgcaaga tgcgctgagg gatgtgcgaa
     3481 aacagtttca gcgcaaccgc ggcgcattta acacgagccc tgatacgctt ccggtattcg
     3541 gcaccatcgc gtcgcgcttc aacgacgacg gcgtgactgc gctctaccag accatcgccg
     3601 cagaactcaa aacccgcggg ctcaacctca cgcagggaca gctcaagact gtcgctgcga
     3661 aatcctcgtc cggtcagaat gtaatcgttc cgcccgcgcg ctcacgctat cttgcggaga
     3721 ttgctgaaac cgtgcgcgct taccacaccg aagtcacgcg gcaaacgaaa actgcgcgcg
     3781 aacgccaaca gctgcgtgaa gcaaaacgaa tgctggaggc cgccaggaag ccttctgccg
     3841 acctcgatgc gctggccgcc gagcgcgata ccgcacttaa cgccgaagcg aaggcattgt
     3901 tagcgcagtg gccgcagatc aagcaggctt actccggcga tgagcaggtt accaaagtac
     3961 gtgaccgcga aatccgcacg acgctcagga ccactacgct ttccggcacg aagataccca
     4021 aagtatcgct gcccacattc gaagat
//
LOCUS       DATMZQ010000443         4534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_897599, whole genome shotgun sequence.
ACCESSION   DATMZQ010000443 DATMZQ010000000
VERSION     DATMZQ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897599"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VN496_14125"
     CDS             <1..584
                     /locus_tag="VN496_14125"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXF18142.1"
                     /translation="AADRLGMAESLLALLATCKVAVRGPKPTEALRASHVRIDLMALE
                     PYTTAQVLESVRGCDLTGARVLVQRYGETNLGLQAALEARRARVVEVPTYRWALPADT
                     RPLERLIDGLAQGQVDAAIFTSASQVRNLFAIAAQRDVSALLRESLNTVLVASIGPVC
                     SHALRAFGVRVSLEATPPKMGPLVEMLTTAWPK"
     gene            complement(625..1182)
                     /gene="greB"
                     /locus_tag="VN496_14130"
     CDS             complement(625..1182)
                     /gene="greB"
                     /locus_tag="VN496_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HXF18143.1"
                     /translation="MNKAFTKENDEEDEDSDPGTPPLPTGLKNYITPAGFKRLKDEAL
                     HLLDKERPELVKVIHWAASNGDRSENADYIYGKRRLREIDRRIRFLTKRLDAAVVVDP
                     AAREPTDQVFFGATVTLADGSGAQKLYSIVGIDEADVSRGRVSWISPLAKALIKAREG
                     ETVVVKTPAGDERLEIVGVAYKAID"
     gene            1500..1805
                     /locus_tag="VN496_14135"
     CDS             1500..1805
                     /locus_tag="VN496_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18144.1"
                     /translation="MDISNRRDSLAMDQWNVVYIPQSRVFEASNRTFLGLLHAENSID
                     FDPTTEYPVYSSAAKLGDGIHYFFSPVASNRFRALLKFWSAVLMEASPDMTLLRRIL"
     gene            complement(2197..2424)
                     /locus_tag="VN496_14140"
     CDS             complement(2197..2424)
                     /locus_tag="VN496_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18145.1"
                     /translation="MNSISWRVVPKGKVWYWEREDTFTVLRTGPFSSYTACAADANWN
                     GFELDKLPEVAQRHPDRKYKPKSTASQPRAN"
     gene            2934..3461
                     /locus_tag="VN496_14145"
     CDS             2934..3461
                     /locus_tag="VN496_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXF18146.1"
                     /translation="MAIKVGDKLPPGTLWEFIEEETPGCSIGPNAFKVEEITPGKRIA
                     VLGLPGAFTPTCSAKHLPGFVRLADEFRRKKVDEIWCLSVNDAFVMGAWGKEQGAKGK
                     VRMMADGSAQYSKALGLEFDLTARGMGVRSQRYSMLVVDGVVKALNVDQPGKFEVSDA
                     ETLLKQAEKVTQFLP"
     gene            complement(3529..4461)
                     /locus_tag="VN496_14150"
     CDS             complement(3529..4461)
                     /locus_tag="VN496_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXF18147.1"
                     /translation="MATPRIILHHFEKSPFSEKVRVVFGIKQIAWSSVLIPRIMPRPD
                     LMPMTGGYRRTPTMQIGADIFCDTQIILRELERRFPVPTLLPGSNAGLGWMLGMWSDR
                     SFFQNTVNLVFGTLADQVPKEFREDREQLRGQQFDVQAMKAAIPQMRDQLRAHVDWIE
                     AQLNDGRTWLLGNAPSLADVDAYMNVWYVRGNLPDAVRLFEAFPNMLAWEQRVKNIGH
                     GLRGPELTSAEALQIARDASPQTASAPDLGDLNGRKPGDRVQVTPDDYGKIPVEGTIV
                     SLSAQHIAIRRNDELAGEVVVHFPRAGFLVTPLG"
BASE COUNT          952 a         1211 c         1333 g         1038 t
ORIGIN      
        1 aggcggccga caggctgggc atggcagagt cattgctggc tctgttggcg acttgcaaag
       61 ttgcagtacg gggaccgaag cccacggaag cactgcgtgc tagccacgtg cgcattgatc
      121 ttatggcgct cgagccttac accaccgcgc aagtgctgga gtcggttcgt ggttgcgatt
      181 tgaccggagc gcgcgtgctg gtacagcgat acggtgaaac caatcttggg ctccaggcgg
      241 cgctcgaggc gcggcgagcg cgggttgttg aggtgccgac ctaccgctgg gcattgccag
      301 ccgatactcg gccattggag cgtctgatcg atggcctcgc gcagggacaa gtggatgcgg
      361 ctatatttac cagtgcatcg caggtgcgca atttgtttgc aatcgccgca cagcgcgacg
      421 tttctgcact cctgcgcgag agcctgaaca cggtgttggt tgcatccatc ggcccggttt
      481 gcagtcacgc gcttcgggca ttcggcgttc gcgtcagcct ggaagccact ccgcccaaaa
      541 tgggaccgct ggtggaaatg ttgactacgg catggccgaa ataaattaac tggtgaattg
      601 aacgatacag tggcggtaag tcgatcaatc gattgctttg tacgccacgc ctacaatctc
      661 cagcctttcg tccccggcgg gggtcttgac gaccaccgtc tcgccttccc gcgctttgat
      721 cagcgctttt gcgagcggcg atatccacga gacacgtccg cgggatacat ccgcttcgtc
      781 gattccgacg atgctgtaaa gcttttgcgc tccgctgccg tcggccagtg ttactgtcgc
      841 cccaaaaaaa acttgatccg taggctcgcg ggcagccggg tccaccacga ccgccgcatc
      901 aaggcgcttg gtcaggaaac ggatgcggcg gtcgatttcg cgcaaacgtc gcttgccata
      961 gatgtaatcg gcattttccg agcggtcccc gttcgaagct gcccagtgga tgactttcac
     1021 gagttcgggg cgctccttgt cgagaagatg cagcgcttcg tccttcagcc gcttgaatcc
     1081 agcgggcgtg atgtagtttt tcagtccggt cggtaagggc ggtgtgccag gatcactgtc
     1141 ctcatcttct tcgtcgttct ctttcgtgaa tgccttgttc atgttgcaat gtggcttgag
     1201 tgtggttagt catgacgagt tgtgcaattg gattgtaatt cgtcagcatc attctgcggc
     1261 gtctgctgat gtgcggaaat acgaagaacg tccgggagcc aacgagtcat ctgttctggt
     1321 cgtccggcat cgttagctaa gttcgcccac ttccgtggac gatgtctttc cgcgaaaggg
     1381 gacgaccgcc gttattggaa taggcacgcc attcaagaac cttgatatag tctgtgcgtg
     1441 agcgcacaga attcgcagct gtcctatgat attcggttgg agtgacctga agctcatcaa
     1501 tggatatatc aaaccgccgt gacagtttgg cgatggacca gtggaatgtc gtctacattc
     1561 cacagagccg ggttttcgag gcatccaacc gaacttttct cggcttgctg cacgccgaga
     1621 actcgatcga cttcgatcca acgacagaat acccagtcta tagttcggct gccaagctag
     1681 gcgacggcat ccactatttc ttctcgccgg tagcatcaaa tcgattccgg gctttgctaa
     1741 agttctggag cgcggttttg atggaagcat caccggatat gacgcttctg cggcgaatcc
     1801 tttaagacca tcggcgcttc ggtcaagtag gggggacctt gatggcgggt tactggcgat
     1861 tcatcgagct acaacgaggt caatgggctt gggagctaca agagccggat ggttctatat
     1921 gcagggtagg tccatttgca agccttgatg cgtgcatgag gaacgccagg gagtacggct
     1981 tccgagagag cgacttggac aggagacagt atcagcgcca ggcagaatca cgcatccgtg
     2041 cttaagttca gattcaggtt ccggctagca ggcacctgaa gtcgagtccc caaaaaaagt
     2101 gaaaggcccg cgacgtcacg ggccttcact ctcctcattt cttcgaatat cagcggtgca
     2161 atgtggtctc ggtcgagcgg taagcactcc aatcaactag tttgctcgcg gctgactcgc
     2221 tgtgcttttc ggtttgtatt tccggtctgg atggcgctgg gcgacttcag gtaacttgtc
     2281 cagttcaaaa ccgttccagt tcgcatcggc cgcgcaagcc gtataagaag agaacggacc
     2341 cgtgcgaaga acagtgaagg tatcttcgcg ttcccaatac cagaccttgc ctttggggac
     2401 aactctccag gaaatgctgt tcatttgatt cccccgtatg ctttgacagt tgtgacggtg
     2461 cccgatattt tcgggtctat tgacccccaa gatgtttggt ggacgcctat ttttatatgg
     2521 tctttttata tggtcctttt ttatatgctc cgttgcgccg tccaccatgc gacacgcgag
     2581 catcataaag aagccgcgat ggaaacatca gcgtgaaggc cgctgatact ggtgtaggag
     2641 ttttactaca tccgcttcgc cccgtcgtcc tacgtgtgac ccatattagt tgctggcccg
     2701 ttagcgaaag aaagcacatt ggtcgggcgg accgacaaag tgcagcaaca gttagaactc
     2761 gaagcacaat ggtactccca tgggttcgac gatggcgtga aggcgaaagc gagtggagat
     2821 acaggctgac atggaggcgc gtggaatcag ccgtcaattc aaaaggatta caatcgcgct
     2881 gacaattgtc taatctcaat ggaagacttc aaccagatgt ggaggatttc ggaatggcta
     2941 tcaaggtagg agacaaactt ccccccggca cgctgtggga attcatcgaa gaggaaacgc
     3001 cgggttgcag catcggcccg aacgcgttca aggtcgagga aatcacgccc ggaaagcgga
     3061 ttgccgtatt gggactgccc ggggctttta cgccgacgtg ttccgccaag catctgccag
     3121 gcttcgtacg gctcgccgat gaatttcgca ggaaaaaagt cgacgaaatc tggtgcctgt
     3181 cggtgaacga cgcttttgtc atgggcgcat ggggcaagga acagggtgcc aaaggcaaag
     3241 tacgcatgat ggcagacggc agcgcacagt attccaaggc gcttggtctg gaattcgatt
     3301 taacggcgcg cggcatgggt gttcgctcgc agcgctattc catgctggtg gtcgacggcg
     3361 ttgtcaaggc gctgaacgtg gaccaacccg gcaagttcga agtcagcgat gcggagacgt
     3421 tgctgaagca ggcggagaag gttacgcaat tccttccctg aacgggccaa tgcatcaagt
     3481 aaggacggca ccagccgtcc ttcttgtggc gcctttggct cgtctggttt atcccagtgg
     3541 cgtaaccaga aatccggcgc gtggaaaatg cacaacgact tcgccggcaa gttcatcgtt
     3601 gcggcggatg gcgatgtgct gagccgacaa cgatacgatg gttccttcca ccggaatctt
     3661 gccatagtca tccggcgtca cttgtactcg atcaccgggc ttgcgtccat taaggtctcc
     3721 gaggtccggt gcactggcgg tttgcggcga agcatcccgc gcaatctgca gtgcttcggc
     3781 ggaagtaagt tcaggtccgc gtaagccatg gccgatattc tttacgcgtt gttcccaggc
     3841 gagcatgttc ggaaaggcct cgaacagccg tactgcatcc ggcaaattgc cgcgtacgta
     3901 ccagacgttc atgtaggcat cgacatcggc caggctcggc gcattgccca acaaccaggt
     3961 acggccgtcg tttagttgcg cttcgatcca gtccacatgc gcgcgcaact ggtcgcgcat
     4021 ctgcgggatc gctgctttca tggcctggac gtcgaattgc tggccgcgca attgctcgcg
     4081 atcttcgcga aactccttcg gtacttgatc ggcgagcgtg ccgaacacca gattcacggt
     4141 attttgaaaa aagctgcggt cgctccacat gccgagcatc catcccagtc cggcgttgga
     4201 acccgggagc agcgtcggca cagggaagcg gcgctcgagt tcgcgcagaa tgatctgcgt
     4261 gtcgcaaaag atgtcggcgc cgatttgcat ggtcggcgtg cgccggtagc cgcctgtcat
     4321 gggcatgagg tcgggacgcg gcatgatgcg cgggatcagc accgagctcc aggcgatctg
     4381 tttgatgccg aacacgacac gtactttttc cgagaacggc gatttttcga aatggtgcag
     4441 aattatgcgt ggcgtcgcca tggagctgcc ttcagccgag aaaggaaata aggttatcga
     4501 gcacctcgtc cggcttctcc gccatcaagg catg
//
LOCUS       DATMZQ010000444         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5023319, whole genome shotgun sequence.
ACCESSION   DATMZQ010000444 DATMZQ010000000
VERSION     DATMZQ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5023319"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VN496_14155"
     CDS             <1..1144
                     /locus_tag="VN496_14155"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXF18148.1"
                     /translation="IEKNACPTVGACGGMFTANTMSTSFEALGMSLMQSSTMASPDAE
                     KADSAAESAKVLVAAIKKNIRPRDIITRESIENVVTLVMATGGSTNAVLHYLAIASAA
                     EVPWTIDDFERVRRKVPVLCDLKPSGRYVATDFHKAGGAPQVLKMLLANGLLHGDCMT
                     ITGRTIAEELSNIPAEPRTDQDVIRPFTNPMYKQGHLAILKGNLAPEGCVAKITGLKN
                     PSITGPARVFDSEPEVMAAIMDRKIKPGDVVVIRYEGPKGGPGMQEMLAPTSALTGQG
                     LGDSVGLLTDGRFSGATWGMVVGHVAPEAYVGGVIALVKEGDSITIDAHKQLIQLNVS
                     EQELAKRRAAWTQPKLRFTRGLLAKYAYLVSSATKGAVTDSFDPKR"
     gene            complement(1168..1860)
                     /locus_tag="VN496_14160"
     CDS             complement(1168..1860)
                     /locus_tag="VN496_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18149.1"
                     /translation="MTPRPAMPISEVVSLSQGSADQAINRVSASKTVYALRGSDFGKL
                     ADAGVPPAVLDYLQQSFVNDVDLLTRYWVLGESLGGCVSCYPQPVDLATLGSGGNGMA
                     DASGLGRYYTYGKPEGLPDWVTAFPGYPNAPGLTIGEVEQMVKEGTPSPDVAARIRAS
                     RLHDIIGTQGLGKISTHFAAGLTGSQLARLHQDGASDEVLDALQQKFLAEYIEFARIR
                     YQSWGKGSNPMH"
     gene            complement(2002..2388)
                     /locus_tag="VN496_14165"
     CDS             complement(2002..2388)
                     /locus_tag="VN496_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18150.1"
                     /translation="MNKVTRKLWMRQGLTAIVVIFWCLGLAGCAGIERRPPPSVDQIV
                     QMSKDGKPADEIIRELQETRAVYPLTASQIVKLHDQGVPESVLDYMQNVYADSIRWDA
                     RMQYESTYWWHNCFYCYPHGVIVVPR"
     gene            complement(2481..2789)
                     /locus_tag="VN496_14170"
     CDS             complement(2481..2789)
                     /locus_tag="VN496_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXF18151.1"
                     /translation="MLAEPAGVIACGNHHGYRVCEERFGQHCREATMDLTMEEAAVVR
                     RKVEMLRVEHRDLDEVIERLSHDQDLDELRLRRLKRRKLLLKDQIALLESRLVPDIPA
                     "
     gene            2953..>3466
                     /locus_tag="VN496_14175"
     CDS             2953..>3466
                     /locus_tag="VN496_14175"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXF18152.1"
                     /translation="MDDITMPQGIYPMLYAFFDPHGGLDSHATRRQVGAFVCNGAHGV
                     AVLGLATEVNKLGDIERRQLVEWVAEELNGRLPLAVTINAPTVQAQVEFAKFAQSLGA
                     SWLILQPPPERNVAEDFFIRFFGSVADRVDLPIAVQNAPEYLGVGLSPEGVNTLHRNH
                     PNFRILKGEGP"
BASE COUNT          752 a          980 c         1005 g          729 t
ORIGIN      
        1 gatcgagaaa aatgcttgtc cgaccgttgg cgcctgtggt gggatgttta ccgcgaatac
       61 catgtcaacg tcgttcgaag cgctcggcat gagtctgatg caatcttcga ccatggcctc
      121 gcccgatgct gaaaaggccg actctgcggc ggagtccgcg aaagtgcttg ttgctgccat
      181 caagaagaac atacgtcccc gcgacatcat cacccgggaa tccatcgaaa acgtggtgac
      241 cctggtgatg gccaccggcg gttcgaccaa tgcagtgctg cactatctgg cgattgcttc
      301 ggcggccgaa gtgccatgga ccatcgatga tttcgagcgg gtgcgtcgca aggtgcccgt
      361 gctttgtgat ctgaaacctt cgggacgtta tgtcgctacg gatttccaca aggccggcgg
      421 tgctccacag gttcttaaaa tgctgctggc gaacgggttg cttcacggcg actgcatgac
      481 aattaccggc cgcaccatcg ccgaggagtt gtccaatatc ccggcagaac cgcgcactga
      541 tcaggatgtg atccgtcctt tcaccaaccc gatgtacaag cagggacatc tcgccattct
      601 gaaaggaaat ctggccccag aaggatgcgt tgccaagatc accggtctga aaaacccctc
      661 cattacggga ccggctcggg tattcgattc ggagcccgag gtcatggcgg ccatcatgga
      721 tcgcaagatc aagcccggag acgtggtcgt gatccgatac gaaggaccga agggtgggcc
      781 cggcatgcag gaaatgctcg cacccacttc agcgctcaca ggacaaggtt tgggcgactc
      841 agtagggttg ctcaccgacg gccgtttctc cggggcaacc tggggcatgg tcgttggcca
      901 cgtggcaccc gaagcctatg tcggcggtgt catcgcgcta gtcaaggaag gcgactccat
      961 taccattgac gcccacaagc aacttatcca gttgaacgtt tcagaacagg aactggcgaa
     1021 gcgccgcgcg gcatggaccc agccgaaact ccgcttcacc cgcgggctgc tggcaaagta
     1081 cgcttacctg gtgtccagtg cgaccaaggg cgcggtaact gattcgttcg acccaaagag
     1141 ataatccaaa tcgctgcggg cgcgtgtcta atgcatggga ttcgatccct tgccccagct
     1201 ttgatagcgg atgcgagcga attcaatgta ttcagcgaga aatttttgct gtaaagcatc
     1261 gagtacttcg tcgctggcgc cgtcctgatg caatcgcgcc agttgcgatc cagtgagacc
     1321 ggcagcgaaa tgcgtgctga tcttgccaag gccttgcgtg ccgataatgt cgtgcaggcg
     1381 cgaagcgcgg atgcgcgctg ccacatcggg tgacggcgtg ccttctttca ccatttgttc
     1441 aacctcgccg atcgtcagcc cgggtgcatt gggataaccg gggaaagcgg tcacccaatc
     1501 gggcaatcct tccggtttgc catacgtgta atagcgaccc aggccgctcg catcggccat
     1561 gccgttgccg ccgctgccga gggtcgccaa gtccaccggc tgcggataac acgatacgca
     1621 acccccgagg gattcgccaa gtacccaata acgggtgagc agatcgacat cattgacaaa
     1681 agactgttgc aaatagtcca gcacagccgg cggcacaccc gcgtcagcca gcttgccgaa
     1741 atccgacccg cgcaaggcat aaacggtctt cgatgccgaa acacggttaa tcgcctgatc
     1801 agcactaccc tggctcaggg acaccacttc cgaaattggc atcgccggcc gtggagtcat
     1861 gatggagcag gcggaggata caagtgcgca tgccaggagg gacgctctga agagcagact
     1921 acgggacggg tagttgacag ccattttgac tccgggtggg taacagcgca tcggatcgac
     1981 acgccctaaa cctgttaacg attaacgcgg cacaacgatg acaccatgcg gatagcaata
     2041 gaagcagttg tgccaccaat aggtactttc gtattgcatg cgggcatccc atcggatgct
     2101 atcagcgtaa acgttctgca tatagtcgag cacggattcg ggaacacctt ggtcgtgcag
     2161 cttgacgatc tgggaagcgg tgagtggata aaccgcgcgt gtctcctgca actcgcgaat
     2221 gatttcgtca gccggtttgc cgtccttcga catttgcacg atttgatcca cagaaggcgg
     2281 cggacgtcgt tcaatgccgg cgcatccagc caaacccaga caccagaata tgacgactat
     2341 cgctgtcagg ccctgtcgca tccacagctt cctggttact ttgttcacgc atagaccttt
     2401 gcatgaggtg ggggttcatc caattctcga ttgccgtgtt acgtagccgc gcgatattgg
     2461 cctcgcgtcg tgtgaactgc ttaggccggg atgtcaggaa cgaggcggct ttccaggagt
     2521 gcaatctgat ctttcagcag gagcttgcgc cgcttcaggc gccgcagtcg cagttcgtcc
     2581 agatcctggt cgtgcgacag tcgttctatt acttcatcca gatcgcggtg ttcgacccgt
     2641 agcatttcca cttttctgcg gactactgcg gcttcctcca tcgtcaggtc catcgtcgcc
     2701 tcgcggcaat gttgcccgaa ccgttcttcg caaacccgat agccgtgatg atttccgcag
     2761 gcgataacgc ccgcgggctc ggcaagcacc gtgttgcaaa tgtaattgcc acaaggcaac
     2821 ggaacaccgg aagattgctc atgtacgaag atgtgcggcg ggcgacatgc aaaaatggta
     2881 caccgttttg gcggctggat cggcaatttg cgggtttgcg gcgcgatcgt gacggtggga
     2941 agggagaccg aagtggatga cattacaatg ccgcaaggca tctatccgat gctctacgcc
     3001 tttttcgacc cgcatggagg gttggacagc catgcaacgc gccggcaagt tggcgccttc
     3061 gtttgcaatg gtgctcacgg cgttgctgtg ctgggccttg ccacagaagt gaataaactt
     3121 ggcgatatcg agcgcaggca attggtggaa tgggtagcag aggaactgaa cggccgtttg
     3181 ccgctggctg taacgatcaa tgctccgacc gtacaggcac aggtagagtt tgcgaaattc
     3241 gcacagtcac ttggcgcatc gtggctgatc ctgcagccgc cacccgagcg caatgttgca
     3301 gaagattttt tcatacgatt tttcggttcg gtcgccgatc gcgtcgattt gccgatcgct
     3361 gtccagaacg cgccggagta tctcggtgtc gggctcagcc cggaaggcgt gaacacgctg
     3421 catcgaaatc atcccaattt ccgcatcctc aaaggtgagg gcccgg
//
LOCUS       DATMZQ010000445         7041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_900132, whole genome shotgun sequence.
ACCESSION   DATMZQ010000445 DATMZQ010000000
VERSION     DATMZQ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900132"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VN496_14180"
     CDS             complement(<1..741)
                     /locus_tag="VN496_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXF18153.1"
                     /translation="MNTSTRFRLVFIALLFLNLASCGDTATLPVQAGMGKDPALPPPH
                     HTWLPTMHVATAKGWPEGGKPSAAAGLQVNAYAMGLDHPRWLYVLPNGDVLVAETNAP
                     PKPEDRKGVKGWVMKKVMAKAGAGTPSANRITLLRDRDGDGIAETRSVFLQGLHSPFG
                     MVLVGDALYVANSDSIMRFHYAADATQITDAGQKIVDLPAGPINHHWTKNVIASADGK
                     RLYVTVGSNSNVAENGMAAEENRAAILEV"
     gene            972..1493
                     /locus_tag="VN496_14185"
     CDS             972..1493
                     /locus_tag="VN496_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HXF18154.1"
                     /translation="MNKLFALGTVLVSSAICWSAHAAAPNDAQIAAIVVAANQVDINA
                     GQLAKSKAQDKEVKAFADRMVTDHTGVNKSAVDLVTKLKVKPEENETSHALKANGADT
                     LKRLKALKGKEFDKAYVDNEVTYHETVLDALDKTLIPNASNEELKSLLVKVRPAFVAH
                     LDHAKHIQATLDH"
     gene            1486..1845
                     /locus_tag="VN496_14190"
     CDS             1486..1845
                     /locus_tag="VN496_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HXF18155.1"
                     /translation="MTSMARPMAAVRILPVVLLAASFCMGADTGRPAAASSNVHTVTI
                     EAMRYLPDRLTVHAGETVVWVNQDLFPHTVTEVNGRFDSHEIQPNASWRYVAKKPGAF
                     DYVCTLHPSMKGALVVK"
     gene            1995..2435
                     /locus_tag="VN496_14195"
     CDS             1995..2435
                     /locus_tag="VN496_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HXF18156.1"
                     /translation="MRTPGSIAGHPIHPMLVTIPIGLWIFSLVCDLIHAGGGTTAWAT
                     VALFTMGGGIVGALAAALFGFIDLLSLPAGPRKTALAHMAINLAVVALYAVNFALRLR
                     AETSEPGKLIWLSVIAIALLGISGWLGGKLVYEHGIGVDTKAIQ"
     gene            2542..3849
                     /locus_tag="VN496_14200"
     CDS             2542..3849
                     /locus_tag="VN496_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HXF18157.1"
                     /translation="MAAPGFIFSTGIENSIPTISDGTIRVDEMEKCGHYSHWRTDFRL
                     VSELGIRFLRYGPPLHKTFRGPDLYDWSFSDDAFNELRRLDLVPIVDLCHFGVPDWIG
                     NFQNPDFPALFADYAYAFARRYPWVQLYTPVNEMSICARFSALYGWWNEQLRDDRSFV
                     NALKHIVKANVLAMHKIISARPDAIFIQSESTEYFHAENPNAIRPAEIMNSRRYLSLD
                     LNYGVRVDSEMYELLMDNGMTRDEYHFFLNNSLKHHCIMGNDYYVTNEHRVASDGATC
                     ASGELFGYAIITKQYYDRYRLPVMHTETNLVQGPVGKEAVNWLWKEWANVLRVRNDGV
                     PIVGFTWYSLTDQVDWDSALRENNGNVNALGLFDLERNIRPVGTAYKTLIESWRAILP
                     TQSVCLQVPVVLPSEHGDKWALEKRIQARQLGPSGAEAPKNAE"
     gene            3881..4600
                     /locus_tag="VN496_14205"
     CDS             3881..4600
                     /locus_tag="VN496_14205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18158.1"
                     /translation="MAIVTGAASGIGLAAAKRLGQEGARLTLVDLRAAPLQEAVAITA
                     QAGAADVWTSVCDVSIESQVEATVNGTIQRYGHLDVVVNNAGLMSFKPLEEFTGTEWN
                     TILGVDLLGAFFFIKHAFRHMKPGGAIVNIASIHAVQTTPLVAPYAAAKAALLSLTRS
                     AALEGRAKGIRTNAILPGAIETPMLNQNPNVRSGVEKIDPTELGNADNIAAAIAYLGS
                     EEAAFVQGAGLIIDGGRLSAL"
     gene            complement(4930..5430)
                     /locus_tag="VN496_14210"
     CDS             complement(4930..5430)
                     /locus_tag="VN496_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18159.1"
                     /translation="MPKTTKFLARAAACLSLLMHLPAGHAYLIGEGDRGTVQIGPYVY
                     HFVDNTDHNQWPRLFGLEYESGSHWLVGGVVFRNSYYQDATYIYGGKRWFVDAPDNHV
                     YLKLTAGPVYGYKPPYDDKLPINHDGFGFGVAPALGYQFKRASVQLVAPGFSVIAITF
                     GYDLWN"
     gene            5936..6424
                     /locus_tag="VN496_14215"
     CDS             5936..6424
                     /locus_tag="VN496_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18160.1"
                     /translation="MNQLSGRGIISHQKSETDDSEFVEVSGNNVLSNAGMSSFGGAML
                     GGRWESITPDIVYITVSYASSANGEPIVIEFDELRINVNGSISKFKPAAPGIISMRLA
                     YLQGMLAADQCTVQLIRGTDVTEGDFTIDSDGGKRTAKADLQRFYDKVRATKADRLAK
                     QI"
     gene            6652..>7041
                     /locus_tag="VN496_14220"
     CDS             6652..>7041
                     /locus_tag="VN496_14220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXF18161.1"
                     /translation="MAPLFESVPPKLYGGTERVVSFLTEELVRQGHAVTLFASGDSVT
                     AARLHACSPQSLRLGLDSGDPFVSQLAHIVLMQEVISQAPLFDIIHFHLDHVHLPAIA
                     GRGLPVVSTLHGRMDLPLLPEIYDHCRD"
BASE COUNT         1592 a         1990 c         1964 g         1495 t
ORIGIN      
        1 aacctccaga atcgctgccc gattttcctc cgctgccatg ccgttttctg cgacgttgct
       61 gttggaccca acggtgacat acagacgctt gccatccgcg ctggcaatga cgttcttggt
      121 ccagtggtga ttgatcggac ccgctggcaa gtcgacaatc ttttggcccg catcggtaat
      181 ttgcgtggcg tcggctgcgt aatgaaagcg catgatcgaa tccgagttgg ccacatagag
      241 cgcatcgccc accagcacca tgccaaacgg cgaatgcagg ccctgcagga acacgcttcg
      301 ggtttccgcg atgccatcac catcccggtc gcgcaacagc gtgatgcggt ttgcgcttgg
      361 cgtgccggcg ccggccttcg ccatgacttt cttcattacc catcccttta cgcctttgcg
      421 atcttccggc ttcggaggcg cattggtttc cgcgaccagc acatcgccgt tgggcagaac
      481 gtataaccag cgcggatggt cgagacccat cgcataggcg ttcacctgta aaccagcggc
      541 cgcactcggc ttgccgcctt ccggccatcc cttggcggtg gcaacgtgca tggtcggcag
      601 ccaggtgtgg tgtggcggcg gcagtgccgg atcttttccc atgccggcct gaaccgggag
      661 cgttgcggta tcgccgcagc tcgcaaggtt caggaacaag agcgcgatga atacgaggcg
      721 gaacctggtc gaggtgttca tgagcaatct ccttgtcgtt ggcagtgaat gccattgaac
      781 ttaagcgatt gactgggcca gtcagtcata gttctcctgg ccgatggagc ctcgtcaact
      841 ccaggcaaga tcgggaacgc agacaaagcg tccttcatcc aatgccgtgc gaaggacagc
      901 ggcgtaacga aggctctgcc tgcgtcgggt tcttgcaccc ggtcatcctt caatcttgaa
      961 aaggtgtttc catgaataaa ttgtttgctt taggaaccgt gcttgtttcg tcggcaattt
     1021 gctggtctgc gcatgccgcc gcaccgaacg atgcgcagat cgccgcgatt gtggtggccg
     1081 caaaccaggt cgatatcaac gccggccaac tggcgaaatc gaaagcccag gacaaggaag
     1141 taaaagcctt tgcagaccga atggtcaccg accatacggg cgtcaataaa tccgccgtag
     1201 acctggtgac gaagctgaaa gtgaaaccgg aggaaaacga gaccagccac gctttgaaag
     1261 ccaacggcgc ggacaccctg aaaagactca aggcgctcaa gggaaaagag tttgacaagg
     1321 cttacgtcga caacgaggtg acctaccacg agaccgtgct ggacgccctg gacaaaacgc
     1381 ttatccccaa tgccagcaac gaagaactca agtcgctatt ggtaaaagtg cgccccgctt
     1441 tcgttgcgca cctcgatcat gcaaagcaca tccaggcgac gttggatcac tagcatggcg
     1501 cgccccatgg ccgcggtaag gatattgccg gtcgtcctgc tcgcggcttc cttttgcatg
     1561 ggcgcggaca ccggcaggcc cgcggctgca agcagcaacg ttcacaccgt tacgatcgag
     1621 gcaatgaggt atcttccgga ccgtctgaca gtgcatgcgg gggaaaccgt agtctgggtc
     1681 aatcaagatc tgtttcccca taccgtgacg gaagtaaacg ggcgttttga ctcgcacgaa
     1741 atccagccaa acgcctcctg gcgatacgtg gctaaaaagc cgggtgcctt cgattacgtc
     1801 tgcaccttgc atccatccat gaagggcgcg ctggtggtca agtaatcggc cacgcttcac
     1861 ggagccgcaa ttgggtggca ggccagtgaa ccggcatgcc ctcgatagtg tccggcgggt
     1921 gcacttcctt ccaactggac tcgccggaga acttccgctc cgagcggtac tcagatggta
     1981 tgaaggagca gccgatgcgc acacccggca gcattgccgg ccatccaatc caccccatgc
     2041 tcgtgacaat accgatcggg ctgtggatct tctcgcttgt gtgcgatctc attcacgccg
     2101 gcggcggcac gacggcatgg gcaacggtcg cattgttcac catgggtggg ggcattgtcg
     2161 gcgcattggc tgcggccctg ttcggcttta tagacctgct ttcgctgccg gccggccccc
     2221 ggaaaaccgc cttggctcat atggcgatca acctcgcggt agtcgcgctg tatgcagtaa
     2281 attttgcgct acgcctgcgc gctgaaacgt ccgaaccggg caagctcatc tggctttctg
     2341 tcatcgcaat cgcgttgttg ggaatttcgg gatggctggg tggcaaattg gtttatgagc
     2401 acggcatcgg agtggacacc aaagccatcc agtgactttt catgctcgct tgatgtggtc
     2461 tcgagctggc tcaaccgcat caatgtctga ccctttgcag gctcctgctt gcgctcatcg
     2521 atcgtcccga atccataaag aatggccgct ccaggattta ttttctcaac cggaattgaa
     2581 aacagtattc cgaccatttc ggatgggacc attcgcgtcg atgagatgga gaaatgcggg
     2641 cactattccc attggcgcac cgacttccgc ctggtaagcg aactcggcat ccggtttttg
     2701 cgctatgggc caccgctgca caagacgttt cgtggcccag acctctacga ttggtctttc
     2761 tccgatgacg ccttcaatga actgcgtcgt ctcgaccttg ttccgatcgt cgatttatgc
     2821 catttcggcg tgccggactg gatcggcaac ttccagaacc cggacttccc tgcgcttttt
     2881 gccgattatg cttacgcgtt tgcacgtcgt tacccctggg ttcagcttta cacccccgtc
     2941 aatgaaatgt cgatttgcgc gcgcttctct gcgctgtacg gctggtggaa tgaacaactc
     3001 cgcgacgacc gatcgttcgt caatgcgctc aagcatatcg tcaaggccaa cgtgctggcc
     3061 atgcacaaaa tcatatccgc ccggcccgac gcaattttca ttcaaagtga atcaacggaa
     3121 tattttcatg cagaaaaccc gaatgccata agacccgccg aaatcatgaa ttcgaggcgc
     3181 tatctctcgc tcgacctgaa ctacggcgtg cgcgtcgact cggaaatgta cgaattgctg
     3241 atggataacg gaatgacgcg cgacgaatac catttcttcc tgaacaacag cctgaagcat
     3301 cactgcatca tgggtaatga ctattacgtc acgaacgagc accgtgtcgc atcggatggt
     3361 gccacttgcg cgtcaggtga actgttcggc tacgccatca tcaccaagca gtattacgac
     3421 cgttatcgct tgcccgtcat gcataccgaa accaatcttg tgcaaggccc ggtcggcaaa
     3481 gaagcggtca actggctatg gaaagaatgg gccaatgttc tgcgcgtgcg caacgacggc
     3541 gttcccatcg ttggatttac ctggtattcg ttgaccgacc aggttgactg ggattccgcg
     3601 ctgcgagaga acaacggcaa tgtcaatgca ctcgggttgt tcgatctcga acgcaacatc
     3661 cggccggtag ggactgctta caagacgctg attgaatcct ggcgcgcgat attgccgaca
     3721 caaagcgtat gcctgcaagt accagttgtc ttgcccagtg agcacggcga caaatgggcg
     3781 ctcgaaaagc gcatccaggc acggcaactc ggacccagcg gagcagaagc gccgaaaaat
     3841 gccgaataac aatctatcca tgcggcggta tgagaacaaa gtggccattg ttactggtgc
     3901 cgcgagcggc atcggtttgg ctgcggcgaa acgcctcgga caggaaggtg cccgtctgac
     3961 gctggttgat ttgcgagcgg cgccgttgca ggaagccgtt gcgatcacgg cgcaggccgg
     4021 tgccgctgac gtttggacta gcgtgtgtga tgtttcaatc gaatcgcagg ttgaggccac
     4081 cgtgaacgga accatccagc gctacggaca cctcgacgtg gtagtcaaca atgccggact
     4141 gatgtcattc aagcctctcg aggaatttac cggcaccgaa tggaacacga ttctgggcgt
     4201 tgatttgctg ggcgcgttct tcttcatcaa gcacgccttc aggcatatga aacccggcgg
     4261 cgcgattgtc aacatcgcaa gcattcacgc ggtccagacc acgccgctcg tggcgcctta
     4321 tgccgcagcg aaggccgcgc tgctgtcgct tacccgctca gcagcactgg aaggcagagc
     4381 caaggggata cgcaccaacg ccattcttcc cggtgcgatc gaaacgccga tgctgaatca
     4441 gaatcccaac gtcagatccg gcgttgaaaa aatcgacccg accgagttgg gtaatgccga
     4501 caacatagct gccgccattg cgtacctcgg ctcggaagag gcggcgttcg tgcaaggagc
     4561 cggtctgatt atcgacggcg gaagattgag tgcgctatag gacacttttg cgcagacgca
     4621 taagccagtg agtatgttcg agcgccggag cataaaaaag cctcctggct cagggaggtg
     4681 gagccaggag gcttacagcc gagccgcgac tagcgacggc ggccgggccg gggggaaacc
     4741 tctcatttca cgcggcagca gcttgccgct gcatccaaag caactatata tgccgggatg
     4801 acggctaaat aatactatat atagtggccc agtcaagtca tttgccaaaa tcttttccgt
     4861 gctccccagc ctttgggagc ttcgttctgg acggtgggac aggtcaaatc gaggcgcgag
     4921 cggtgtggat caattccaca ggtcgtagcc gaatgtaatc gcaataaccg aaaaacccgg
     4981 cgcaacgagc tgcacacttg cgcgcttgaa ctggtagcca agcgcgggcg cgactccaaa
     5041 accaaaacca tcatggttaa tcggcagctt atcgtcatag ggtggtttgt acccatagac
     5101 cggccccgcc gtcagcttga ggtaaacatg gttatcgggc gcgtcgacga accaccgctt
     5161 cccgccataa atgtacgtgg cgtcctgata ataggaattc ctgaagacaa ctccgccaac
     5221 gagccaatgg ctgccggact cgtactcgag tccaaacagc cggggccact ggttatggtc
     5281 ggtattgtcg acaaagtgat agacgtaggg cccgatttgt acagtgccgc gatccccttc
     5341 cccgatcaga tatgcatgac cggcgggcag atgcatgagc aacgacaggc acgctgcggc
     5401 gcgcgccaga aactttgttg tttttggcat gatcttcctt tttcgctgtg cactaaagca
     5461 caagcagtgc tgcgcaaaat acgacttcgg atggtctatt cagcgcacat ttgtgcaaac
     5521 gggttggcga aaagagacgt cacaattagc gaacgtggcc aaatgcacgc ctacagccgg
     5581 taaacagact gtcggtcaac ttgcggaaaa gatggccggc acccagaaag gcgccaacgc
     5641 gacaaggagc gtaaccaacg tcccaaccac ataagtcaat tcgtccaggg cattcacgac
     5701 ttcggcaaca gcacaactta ttccatcgca ctaagccccc gattggtgag aatttaagcg
     5761 ccaaggtctc ccgaactcac cgcgatggca ctttgaacag gaagagttac agcaaattgt
     5821 agaaactttt atttgggatt gggaggcggc gcacttccca aaaggaaacg aaatggttcg
     5881 gttcatttta tccatagcaa ttatcacgtg cctcctcgtg ggctgcgcag ctgtgatgaa
     5941 ccagttgtcc ggtcgcggaa tcatcagcca tcaaaaatca gagaccgacg acagcgagtt
     6001 cgtggaagtg tcggggaaca acgtgctgag caacgcggga atgtcgagtt tcggaggtgc
     6061 gatgctggga ggacgatggg agtccatcac gcccgacatc gtctatatca ctgtgagtta
     6121 tgcatccagc gcgaatggcg aaccgatcgt catcgaattc gatgaactgc ggatcaacgt
     6181 aaatggaagc atttccaaat tcaagccggc cgcgccagga attatcagca tgcggcttgc
     6241 gtatctgcag ggcatgcttg cagctgatca atgcacggtc caactcatcc gcggaacaga
     6301 cgttaccgaa ggtgacttca cgatcgattc tgacggcggc aagcgcacag cgaaagcaga
     6361 cctccaacgc ttttacgata aagttcgcgc cacaaaagct gaccgcctgg caaagcagat
     6421 ttagttcaga gggcctttga gtcgcagcgc acctcgagcc ctgcatccca atctgacagg
     6481 cttctagccc ggttatctga caatgcgctg taaaagcgcg tccaagtgtc aggaactgtt
     6541 gcacgtgtca gcgatctgaa actgggtggt ccctgttctc agcaaagtga cgaagtggcc
     6601 gttagcccct tacctgtagg aaagctgaaa agcgcgctga atatagcgat tgtggcgccg
     6661 ctgtttgaaa gcgttccacc caagctctac ggcggaactg aacgggttgt ttcttttttg
     6721 actgaagaat tggttcgcca gggacacgct gtcacgctgt tcgcgtccgg tgattcggtg
     6781 actgcggcgc ggttgcatgc ctgctcgcct caatctctgc ggctcggatt ggacagcggc
     6841 gacccttttg tctcgcaact ggcccatatc gttctgatgc aggaagtcat cagccaggcg
     6901 cccctgttcg atattatcca tttccatctg gaccacgtgc atttgccagc catcgcgggg
     6961 cgagggttgc cggtagtaag taccctgcac ggacgcatgg atctgccgct gctgcccgag
     7021 atctacgatc attgcaggga c
//
LOCUS       DATMZQ010000446         8257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5024058, whole genome shotgun sequence.
ACCESSION   DATMZQ010000446 DATMZQ010000000
VERSION     DATMZQ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5024058"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VN496_14225"
     CDS             <1..819
                     /locus_tag="VN496_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF18162.1"
                     /translation="PAGLFLANLAVLGFSWLSGDQFLQWGWRIPFFLSIFLIAIGLYI
                     RLNILETPTFARLLAENRIERAPMVEVIKRHPREIILSALARMAEQAPFYIFTAFIFT
                     YATLQLKTSRDFILTALLAASVFSFFSVPFSGWLSDRIGRKNMYMIGAVTTGVFGFAY
                     FAMLDTMIPQWIFIAIFISLIPHDMMYGPQAALIAESFTGRLRYSGASLGYQLASIIA
                     GGPAPLIAAALFAHYHSGYAIAAYIAACAIISLLATAMLRDYTNKDISREYDGK"
     gene            841..1650
                     /locus_tag="VN496_14230"
     CDS             841..1650
                     /locus_tag="VN496_14230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF18163.1"
                     /translation="MTDFEAIKRQQQHTWALGDFAMIGWNTVYPGELLCEAVDLRAGE
                     RVLDVATGSGNTALSAARRNCDATGIDYVPALIERARQRAAVEHLSAKFEVADCEEIP
                     FADANFDVVLSVYGSMFAPDAQRAAGELLRVCRSGGRIGMANWTPEGFWGQTFALVAQ
                     YLPPPAGVRAPAEWGTEKRLTELFGQAVAATRVARRSALFRFRSSAHWIEVFSRYFGP
                     IIRVRETLDDKRRKDFFLELDATLNRFNVSGDDTLVVSADYLEVVMTKRLS"
     gene            1796..2689
                     /locus_tag="VN496_14235"
     CDS             1796..2689
                     /locus_tag="VN496_14235"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF18164.1"
                     /translation="MAQQTFGFIGVGRMGGLMSARLLDAGHQVVIYDVAEEAVAVLEK
                     RGARRAASAAAVAGLAETVFLSLPNPEIVQRAVLDHAGIIEGAKVKHVVDFSTIGPRT
                     AGLVAKGLATRNVIYVDAPVSGGLKGAREGTLAVMVSCPKAIYAQLEAILKTFGKPFH
                     LGENAGQAQTMKLANNLLAAAAMALTSEAMVMGVKAGLDPKVMLDVLNAGSGRNSASQ
                     DKFPKSVLPGTFDFGFATGLSYKDVRLCIDEAEALGVPMVCGAAVRQMLAVTNAMYGP
                     DSDFTSMCKVVESWAGVEVRS"
     gene            2730..4124
                     /locus_tag="VN496_14240"
     CDS             2730..4124
                     /locus_tag="VN496_14240"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXF18165.1"
                     /translation="MSIATQAMNKPLITEAHEPLAARMARSVVAFSGDNLPAEVKAKV
                     KLCLMDLIGCAFESRALPWSRQAQSIAERASNGGTGATIIGAPGAFAFGDAAFVNAVM
                     GHGLVREDMHAGSISHLGIAVLPALLALSQYRRSTGRDFMAAAVAGYEAGGQVGRAAM
                     DAEVAAIHRPTGITGPIAAAAAGARLLGFSVEQATSAIALGANTTAGFNQWAHTGGSE
                     MWFQAGFAARNAVAAVRLAESGAFTSPTALDGEAGLFAALKKRSASSRVQLFSSAPEI
                     LSVYHKPVPACNFAQTASQAALRLARNGTAAERIASITIRVPRAGALYPGCDYTGPFA
                     HVLQAKMSIQYNVAAALLEGGVSEKNFKLLNDARLHKLLGLMNLEIDDQMTRDYPGKQ
                     GGEVELRDAGGAVQRLRLEDVINATPTEVRERFRSATEEAVGKARAQEIEQAIETLDR
                     SDDAASLACLLNAR"
     gene            4192..5151
                     /locus_tag="VN496_14245"
     CDS             4192..5151
                     /locus_tag="VN496_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF18166.1"
                     /translation="MNIAQFLENFLQSLIAGLLTGSIYGLMCVGLSVIFGVMRVINFA
                     HGDFMMLGMYAAYYAFGLLGIHALFGSAAGPYVAALLAGPVIFLAGVLAHRLLIGKVT
                     GMRGSVLQSEGHYAQLILTLGLALILQNGGLYLFGSTPVSIATPLASNAWPVGPLYAD
                     KIEVFVNQGQMVAAVIAVVVVVGFVLVMNRSRLGKSLRAAADNPEAATYMGIDVERSH
                     RLAFGFGVGITAIGGGLLASGTSFQPYVGLEYVIVMYAGVVLGGLGSVPGAFFGGLTI
                     GLVQQLSTLFLPNQLQNTAIFVFFLFIVLLRPQGLFGAALRRT"
     gene            5153..6919
                     /locus_tag="VN496_14250"
     CDS             5153..6919
                     /locus_tag="VN496_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXF18167.1"
                     /translation="MLDVNWRRQTLLIGAFAVAYGVLAAFVEHSYYLLILAVVPVWAV
                     MGLAWNLLSGYSGFVSFGHAAFFGLGAYFVALAQIHWGLSPWLGIPGAAVVGACAGLV
                     VGIPTFRLRGHYFALAMLAYPLALLYVFEWLGYQELALPMQRDHAFAYMQFENQHLYV
                     WLALAMMVGAMLLTGRVERSRFGMALVAIREDEAAAEAAGIRTLAWKLKAITLSGALA
                     GAAGGFYAVVLLVVTPPAVFGMLVSAQALIVAMFGGVATLWGPVIGAVVLVPLSEILH
                     AQLGNIIPGIQGVVYGVAIVAVILLAPEGVFWKVKDMLVRRRERSVPELAAAIVADVV
                     VLPVNRHRSDGPIAMEVRSVSKAFGGLQAVRDVSIRITRGEILGIIGPNGAGKTTLFN
                     LLNGFIATDQGDILFEGVDIAGRRPYEICAAGVGRTFQVVRPFRRMSVLENVVIGAYV
                     HCATDAQARATAEAALAQVGLQYQQHKSASMLTNKELRLLEIARALAGQPRLLLLDET
                     LAGLGAAEVDEIIAVVRKLATQGITIVIIEHTMQAMVRLVDRFVVLNEGALLAEGLPQ
                     DITRNSAVIDAYLGKRWRAANA"
     gene            6912..7619
                     /locus_tag="VN496_14255"
     CDS             6912..7619
                     /locus_tag="VN496_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18168.1"
                     /translation="MLEIENVSAGYGAVPVLNSVSLRVKRGEFAAIVGPNGAGKTTLF
                     KTISGIVRPTSGNIRFDGHDLLAIAPSARAHLGIAHVPEGRQIFPSLTVLENLEMGAY
                     TPAGRHAWKENLEKVHQWFPVLAQRASARAGELSGGQQQMLAIARGLASSPKLLMLDE
                     PSMGLAPTAADEIFERIVAIHSGSGLTVLLVEQRVAEALHYADRGYVLEGGRVVLEGS
                     HDELKSNDRIRQAYLGM"
     gene            7653..>8257
                     /locus_tag="VN496_14260"
     CDS             7653..>8257
                     /locus_tag="VN496_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18169.1"
                     /translation="MGKTIMFAQKRVLGLLVAAALCTALDSGAQTKEVEVGLIAPMSG
                     PYARQGQVMKMAADMAIETINQQGGIKALGGAKLKLEIFDAGDSVEKAKNAAQRMVAD
                     APNLVVATGAYLSSFTLAVTEVTERAQLPVLTFSYSDLITSRGFKYVFQTSATGVQQA
                     EGSLPALIDMAQKASGQRPKTVGIVMDNTAASVSFVKPMRD"
BASE COUNT         1508 a         2348 c         2682 g         1719 t
ORIGIN      
        1 cctgccggac tgtttctcgc caatctcgcg gtgctgggct tcagctggct gtcgggggat
       61 cagttcctgc agtggggatg gcgcatacca ttcttcctca gcattttcct gattgcgatc
      121 ggactctata ttcgcctgaa cattttggag acaccgacat ttgcgcggct gctggcagag
      181 aaccggatcg aacgcgcgcc gatggtcgaa gtcatcaagc gccatccgcg cgaaattatt
      241 ttgtcggcct tggcccgcat ggcagagcag gcaccgttct atatctttac tgcgtttatt
      301 tttacgtatg ccaccctgca gctaaagacc tcacgggatt tcatcctgac tgcgcttctg
      361 gccgcctcgg tattttcatt cttcagcgtg ccattcagcg gctggctgtc cgaccgcatc
      421 ggccgcaaga acatgtacat gatcggtgct gtcaccaccg gcgtgttcgg attcgcctac
      481 ttcgccatgc tggacaccat gataccgcaa tggattttca tcgcgatttt catctcgctg
      541 atcccgcacg acatgatgta tggcccgcag gccgcattga ttgcagaatc gttcaccggc
      601 cggttgcgat acagcggcgc gtcgctcggc tatcagctcg cctccatcat tgccggtggc
      661 ccggcgccgt tgatcgccgc cgcgttgttc gcgcattacc actcgggcta tgcaattgcg
      721 gcctacattg ccgcgtgcgc gatcatcagc ctgctcgcaa cggccatgct gcgcgactac
      781 accaacaagg atatctcccg ggaatacgat ggcaaatgat ctggcaggaa ggcggcatgc
      841 atgactgatt tcgaagcaat caagcgccag caacagcaca cctgggcgct gggtgatttc
      901 gcgatgatcg gctggaacac ggtttatccg ggcgagctgc tgtgcgaggc ggtggacctg
      961 cgcgccggcg aaagagtgct cgacgtggcg actggcagcg gcaacacggc gttgtccgcg
     1021 gcacgccgca attgcgacgc gactggcatc gattatgtgc cggcgttgat tgagcgcgcg
     1081 cgtcagcgtg cagcggtgga gcatttgtcc gcgaaatttg aagtcgccga ctgcgaggaa
     1141 attccgtttg ccgacgcgaa tttcgacgtc gtgctgtccg tctacggctc gatgttcgcg
     1201 cccgatgcac agcgggctgc gggcgagctg ttgcgcgtat gccgctcggg tggccgcatc
     1261 ggcatggcga actggacacc ggagggcttc tgggggcaga cctttgcgct cgtggcgcaa
     1321 tatcttccgc cgccagcggg ggtgcgagca ccagccgaat ggggaacgga aaaacgtctc
     1381 acggaattgt tcggccaagc ggtcgcagcc actcgcgtgg cacgacgcag cgccctgttt
     1441 cgcttccgca gcagtgcgca ctggatcgag gtgttcagcc ggtattttgg cccgatcatc
     1501 cgcgtgcggg aaacgctgga cgataagcgt cgcaaggatt ttttcctgga actggacgcg
     1561 acgctgaacc ggttcaatgt atcgggtgat gacacgctcg tggtgtcggc ggactatctc
     1621 gaagttgtca tgaccaagcg cctctcttga tgcaggtcgg gcggacgccg gctccggatg
     1681 ttgcgccgcg gcgcccgtgt tatgtcagga ataaccagtc tggtatgccg gcagcgggga
     1741 tgcttgccgc tgcgcatccc gttaacatgg cgcacattca tcgtgagggc ttgccatggc
     1801 acaacagacg tttggattca ttggagtcgg ccgcatgggc gggctcatgt cggcgcgctt
     1861 gctggacgcc ggccatcagg tggtcatcta cgacgtagcc gaggaagcag tggcagtgct
     1921 ggagaaaagg ggcgctcggc gtgcagcgtc cgctgcggca gtcgccggcc tggcggagac
     1981 ggtattcctg agtctgccga atcccgagat cgtgcagcgc gcggtccttg accacgcagg
     2041 aatcattgaa ggcgccaagg tcaaacacgt ggtggatttt tccaccattg gtccgcgcac
     2101 agcgggtctg gttgccaagg ggcttgccac acgcaacgtc atctacgtcg atgcgccggt
     2161 cagtggcggg ctcaagggcg cgcgcgaggg cacgctcgcc gtcatggtgt cctgcccgaa
     2221 ggcgatctat gcgcagctgg aagccatcct gaagacattc ggcaaaccgt ttcatctggg
     2281 cgaaaacgca gggcaggccc aaaccatgaa gctcgccaat aatctgctcg cggccgctgc
     2341 gatggcattg acatccgagg cgatggtcat gggtgtgaag gccggactcg atccgaaagt
     2401 catgctggac gtattgaatg ccggatcagg acgcaacagc gcttcccagg acaaatttcc
     2461 gaagtcagtg ctgccgggaa cgttcgattt tggcttcgct accggacttt cctataagga
     2521 tgtgcgcctt tgcatcgacg aagctgaagc gctcggcgtg ccgatggtct gtggcgccgc
     2581 cgtgcgccag atgcttgccg tgaccaatgc aatgtacggc ccggattcgg atttcacgtc
     2641 gatgtgcaaa gtggtcgagt cgtgggctgg cgtcgaagtg cgcagctgat gcgcgccgga
     2701 caacgctaaa cttcaacagg agatctgaca tgtcaattgc gacgcaagcg atgaacaagc
     2761 cgctgatcac ggaagcgcac gaaccgctcg ctgcacgcat ggcgcgcagc gttgttgcgt
     2821 tctccgggga caatttgcca gccgaagtga aggccaaggt gaagctgtgc ctgatggacc
     2881 tgattggttg cgcatttgaa tcgcgcgcac tgccctggag ccgtcaggct cagagcatcg
     2941 ctgaacgcgc aagcaatggc ggcactggtg cgaccatcat tggagcgccc ggtgcctttg
     3001 cctttggcga tgccgcgttc gtgaatgcag tcatgggcca tggcctggtg cgggaagaca
     3061 tgcatgccgg aagcatcagc catctgggta ttgcagtatt gcctgcactg ctggcgctgt
     3121 cgcaatatcg ccgcagcacc gggcgcgatt tcatggccgc cgcggtggcc ggctatgagg
     3181 cgggcgggca agtcggcaga gcggccatgg atgcagaagt tgccgcgatt catcgtccga
     3241 ccggaattac cggcccgatc gcggcggctg cagcaggtgc gcgcctgctc ggattcagcg
     3301 ttgagcaggc aaccagtgcg attgcgcttg gcgccaatac aacggccgga ttcaatcaat
     3361 gggcccacac cggcggcagc gaaatgtggt tccaggctgg ttttgccgcg cgcaatgccg
     3421 tagccgccgt ccggctggcg gaaagcggcg ccttcacttc cccgacggcg ctggacggcg
     3481 aagccggact gtttgccgcg ctgaaaaagc gttcggcgtc ctcccgggtg cagttattct
     3541 caagcgcgcc cgagatcctg tccgtctatc acaagccggt gccggcatgt aatttcgcac
     3601 agactgccag ccaggctgcg ctgcggctcg cgcgcaacgg aacggcggcg gagcgcattg
     3661 catccatcac gatccgcgtg ccgcgcgccg gcgcactgta cccgggttgc gattacacgg
     3721 gaccgtttgc gcatgtcctg caggccaaga tgagcatcca gtacaacgtc gccgcggcat
     3781 tgctggaagg cggcgtatcg gaaaagaatt tcaaactgct caacgacgcg cgactgcaca
     3841 aactgctcgg gctgatgaac ctggagatcg atgaccagat gacaagggat tatccaggca
     3901 agcagggcgg cgaagtggaa ctgcgcgacg ccggcggtgc cgtgcagcgc ctgcgactgg
     3961 aagatgtcat caacgccacc cccacggagg ttcgtgagcg tttccggtcg gcaaccgaag
     4021 aagcggtggg caaggcgcgt gcgcaggaaa tcgaacaggc aatcgaaaca ttggaccgca
     4081 gcgacgatgc cgcgagcctg gcgtgcctgt tgaacgcgcg atgaccgtat tgcgcgcttt
     4141 gactagggtt cggcgaaggg tcgctgcgcg agcaaccttc ggcagagtgt cgtgaacata
     4201 gcgcaatttc tcgagaattt tctgcagtcg ttgattgcag gattgctgac gggctcgatc
     4261 tacggcctga tgtgcgtggg gctgagcgtc atcttcggcg tcatgcgcgt catcaatttt
     4321 gcgcacggcg atttcatgat gctcggcatg tacgcagctt attatgcgtt tggcttgctc
     4381 gggatccatg ccttgtttgg cagcgcggct ggaccttatg tggccgcatt gctggcgggc
     4441 ccggtgattt ttctggcggg tgtactggcg caccggctgc tgattggcaa agtcaccggc
     4501 atgcgcggga gtgttctgca gtccgaaggc cattatgcgc aattgatact tacgctgggg
     4561 ctcgcgctga ttctgcagaa cggcggcctc tatctgttcg gctccactcc ggtttcgatt
     4621 gccacgccgc tggcatccaa tgcctggccg gtgggaccgc tgtacgcaga caaaatcgaa
     4681 gtcttcgtca atcagggaca gatggtcgca gcggtcatcg ctgttgtagt ggtagttggg
     4741 tttgtgctgg tcatgaatcg ttcgcgcctc ggcaagtcct tgcgggcggc cgcggacaat
     4801 ccggaagcgg ccacgtacat gggcatcgat gtggagcgct cgcaccggct cgcgttcggc
     4861 ttcggcgtgg gcatcacggc cataggcggc ggcttgctgg catcgggtac ttcttttcag
     4921 ccttatgtcg gcctcgagta cgtcatcgtc atgtacgccg gcgtggtact gggcggcctg
     4981 ggcagcgtgc ccggcgcttt cttcggcggg ctgaccatcg gcctggtgca gcagctgtct
     5041 acgctgttcc tgccaaatca actgcagaac acggcaattt tcgtgttctt cctgttcatc
     5101 gtgctgctgc gtccccaggg attgttcggt gcggcgttga ggcgcacctg acatgctcga
     5161 tgttaactgg cgccgccaga cgctgctgat cggagcgttt gctgttgcgt atggcgtact
     5221 ggcggcgttc gtcgagcatt cctattacct tctgattctg gccgtggtcc cggtctgggc
     5281 cgtcatgggt ctggcgtgga acctgttgag cggctacagc ggattcgtct cgtttggcca
     5341 tgccgcattt ttcgggttgg gcgcctattt cgtcgccctg gcgcaaattc actgggggct
     5401 ttcgccctgg ctcggcatac ccggcgccgc agtggtcggt gcatgtgccg gactggtggt
     5461 cggtattccg accttccgcc tgcgcggtca ctattttgca cttgccatgc tggcctatcc
     5521 gctggcgctg ctttatgtgt ttgagtggct cggttatcag gaactcgcac tgcccatgca
     5581 gcgcgaccat gcgttcgcct acatgcaatt tgaaaatcaa catttgtatg tgtggctggc
     5641 gctggcaatg atggtcgggg caatgctgtt gaccggccgg gtcgaacgct cgcgtttcgg
     5701 catggcgctg gttgcgatcc gcgaggatga agcggccgca gaagccgccg gcattcgcac
     5761 gctggcctgg aaactcaaag cgatcacgct gagcggcgcg ctggccggtg cggcaggggg
     5821 cttttatgcc gtagtgctcc tggtggttac gccacccgca gtgttcggca tgctggtttc
     5881 ggcacaggcg ctgattgtcg ccatgttcgg cggcgtagcg acgttatggg gaccggtcat
     5941 aggtgccgtc gtgctggtgc cgttgagcga aatcctgcat gcgcaattgg gcaatatcat
     6001 tccgggcatt cagggcgtgg tctatggcgt tgcgatcgtg gcggtgattc tgctggcgcc
     6061 ggaaggcgtg ttctggaaag tcaaagatat gctcgtgcgc cgccgcgaac gttcggtacc
     6121 cgagctggca gctgcgattg ttgccgatgt tgtggtgttg ccggtcaaca gacaccgttc
     6181 tgacggaccg attgcaatgg aagtgcgcag tgtctcgaaa gccttcggcg gcttgcaggc
     6241 ggtgcgcgac gtcagcattc gcatcacgcg cggcgaaatc ctcggcatca ttggaccgaa
     6301 tggagccggc aagaccacgt tgttcaatct tctgaacgga ttcattgcca ccgaccaggg
     6361 agacattctg ttcgaagggg tcgatatcgc tggccggcga ccctatgaga tctgtgccgc
     6421 gggcgtgggg cggacgttcc aggtcgtgcg tcctttccgg cgcatgagcg tgctggagaa
     6481 cgtcgtgatt ggcgcgtacg tgcattgcgc cactgacgcg caggcgcggg cgaccgccga
     6541 agccgcgctg gcacaggttg gcttgcagta ccagcaacac aaatccgcga gcatgctgac
     6601 gaacaaggag ctgcgcctgc tggagatcgc aagggccctt gccggacagc cgcggctgct
     6661 gctgctggat gaaacactgg cgggcctggg ggcagcagaa gtggatgaga tcatcgctgt
     6721 ggtgcgcaag ctggcgacgc aaggcattac gattgtcatc atcgaacaca ccatgcaggc
     6781 catggtgcgg ctggtggatc gttttgtcgt gctgaatgaa ggcgcgttgc tggcggaagg
     6841 actgccgcaa gacatcacac gcaacagcgc ggtgatcgat gcctatctcg gcaagcgctg
     6901 gagagccgcc aatgcttgaa atcgaaaacg tgagcgcggg ctatggagcc gtgccggtac
     6961 tgaactcggt cagcttgcgc gtgaagcgcg gcgaattcgc cgcgatagtg ggccccaacg
     7021 gcgccggcaa gacgacgttg ttcaaaacca tatccggcat cgtgcggccg acttcgggca
     7081 acatccgttt cgacggccac gatctgctgg cgattgcacc ttcggcgcgg gcacatctcg
     7141 ggattgcgca cgttccggag ggacgccaga tattcccgtc gttgacggtg ctggaaaatc
     7201 tcgagatggg agcgtatacg ccagccggac gccatgcctg gaaagaaaac ctggagaaag
     7261 tccatcagtg gtttccggtg ctggcgcagc gcgcatccgc gcgcgcgggc gagttgtccg
     7321 gaggacaaca gcaaatgctg gccattgcgc gcggactcgc gtcatcgccg aagctgctga
     7381 tgctcgatga gccttcgatg ggtctcgcgc caacggccgc cgacgagatc ttcgaacgca
     7441 tcgttgccat ccattccggc tccggtctta ccgtgctgct ggtggaacag cgcgtggccg
     7501 aagcgctgca ttacgccgat cgcggttacg tactggaggg cggccgcgtc gtgctcgaag
     7561 gcagccatga cgaactgaaa agcaacgacc ggatacgcca ggcgtatctc ggcatgtagc
     7621 aagatctacc tacacaaacc gggagaatca tcatgggcaa gacaatcatg ttcgcgcaaa
     7681 agcgtgtgct ggggctgttg gtggcggctg cgctgtgcac ggcgctcgat agtggcgcgc
     7741 agacgaaaga agtggaagtc ggactgatcg cgccgatgtc cgggccgtat gcgcgccagg
     7801 gccaggtcat gaagatggcg gccgatatgg cgatcgaaac catcaaccag caaggaggta
     7861 tcaaagccct cggcggcgcg aaactcaagc tcgagatttt cgatgccggt gacagtgttg
     7921 aaaaagcgaa gaatgccgcg cagcgcatgg tggcggatgc gccgaatctg gttgtggcca
     7981 ccggagccta tcttagttct ttcacgctgg ccgtcactga agtcaccgag cgcgcgcaat
     8041 tgccggtgct gacgttttcc tattccgatt tgattacgtc gcgcggtttc aagtatgtgt
     8101 tccagacatc cgcgaccggc gtgcagcagg ctgaaggatc gctgccggca ttgatcgaca
     8161 tggcgcagaa ggcctccggc cagcggccaa agacggtcgg cattgtcatg gacaacaccg
     8221 ctgcatccgt atcgttcgtc aaaccgatgc gggacca
//
LOCUS       DATMZQ010000447         4247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4703417, whole genome shotgun sequence.
ACCESSION   DATMZQ010000447 DATMZQ010000000
VERSION     DATMZQ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4703417"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VN496_14265"
     CDS             complement(<1..541)
                     /locus_tag="VN496_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXF18170.1"
                     /translation="MIYLNGEFMPIEQARIPVLDRGFIFGDGVYEVIPVYSRLPFRLA
                     QHLQRLQHSLNSISLANPHDDPSWKRLIERLVQLNEPEDQSIYIHITRGPAKRDHAFP
                     ATITPTVFVMSNPLTTPPLRQVKDGVCAITAVDNRWWRCDIKAISLLPNVLLRQQAVE
                     AGCAETVLLRDGFVTEGAAS"
     gene            complement(538..1695)
                     /locus_tag="VN496_14270"
     CDS             complement(538..1695)
                     /locus_tag="VN496_14270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXF18171.1"
                     /translation="MIKFLALIAFLSGSLAFAEGLPGSSAPVPSLSSRAYLLIDFQSR
                     QTLAARNPDDRIEPASLTKLLTAYVVFDALRQKQITLVQPFTVSQKAWRAPGSRMFLT
                     INESVPVEALLNGMIVQSGNDAAIALAEGISGSEEAFVEAMNREAARMGLKNSHFVNA
                     TGLPNAQHFSTAADMATLSRAIIDEFPQYFAMYKVREYRHNNITQYNRNRLLGRDPFV
                     DGLKTGYTTSAGYCMVASAQRNERRLIAVVIDAGSENGRAVDAQKLLNYGFEAFDTIR
                     LYSAGQPVHSLPVWKGNSNELHSGLASDYFVTLPRGFSERLKATMESMQPLMAPIRIG
                     DRVGTLKLTLDGKPFDEQTVIALENVGIANVFIRTWHSLRLLYRKFSGAFA"
     gene            1792..2277
                     /locus_tag="VN496_14275"
     CDS             1792..2277
                     /locus_tag="VN496_14275"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HXF18172.1"
                     /translation="MTTFFPKQSVQRMLILLCLCVGTPAQAAELVKILDPWARATVPG
                     QKVGGVYMEIVARENLRLTGIRTTAAESAQVHVMKMENGMMRMRAVPFLQLPAGKPVK
                     LTPGGYHIMLYDLRRSLVSGQELSLELTFEDAAKQQYRVPVKVIIRDREAGVSYGHEA
                     Q"
     gene            complement(2278..3063)
                     /locus_tag="VN496_14280"
     CDS             complement(2278..3063)
                     /locus_tag="VN496_14280"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXF18173.1"
                     /translation="MKQLAAIESLLQAGILAGDGAIEGEIEGSSDEFRRIRLGIYRDA
                     YRLRLLEVLGKDYPVSREYLGDESFDAAALEYLDAYPSTFRNVRWFGGQLAEFLGSTL
                     PYSENPLLAELAHFEWTLGLAFDAADSQSVSFEDVSTVPAHLWPEMRFLPPAALHLVE
                     LTTNAVAIWKATGDKGRIPVQVSAEPVTWVIWRKDDTPLFRSLEADEAWALKALLAGA
                     SFEEICGGLCQWFPEDKAAARAAGTLRRWVDEGWIAKLSFVNG"
     gene            complement(3060..3893)
                     /locus_tag="VN496_14285"
     CDS             complement(3060..3893)
                     /locus_tag="VN496_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXF18174.1"
                     /translation="MASVQGFGLGLRTEHYQPILESHPRVDWLEAVTENYLVPGGKPL
                     HYLDRIRADYPVALHGVSLSIGSADPLDYGYLKALKHLMQRTEPGWISDHLCWTGIAG
                     KNLHDLLPIPYTEEAVGHIAARVRQVQDFLGSQILLENVSSYLAFRGSEMSEWHFLAE
                     IARRADCHILLDVNNVYVSAFNHGFDAIEYLDGIPIDRVRQIHLAGHRHCGTHIIDTH
                     DADIIDPVWELYASALRRFGNVATMIERDDQIPPLAELVSELNHAREIAERTLPAKVA
                     A"
BASE COUNT          971 a         1182 c         1181 g          913 t
ORIGIN      
        1 tgctagcggc tccctcagtc acgaaaccgt ctcgcagcag caccgtttca gcgcatcccg
       61 cttcgactgc ctgctggcgc agcagcacat ttggcaataa cgagattgcc ttgatatcgc
      121 aacgccacca gcgattgtct accgcagtga tcgcgcatac tccatctttt acttgccgca
      181 gcggaggtgt cgtcagcgga ttgctcatga cgaatacagt cggtgtgata gtggctggaa
      241 aggcatggtc ccgcttcgcc ggtccacggg tgatatgtat gtagatcgat tgatcctcag
      301 gttcgttcag ctgcacgagg cgttctatca ggcgtttcca gctgggatca tcgtgcggat
      361 ttgcgagcga gatactgttc agactgtgct gaaggcgttg cagatgttgg gccagccgga
      421 aaggaaggcg cgaatagacc ggaataacct cgtaaacgcc gtcgccaaaa ataaagcctc
      481 ggtcaagcac cggaatgcgt gcctgctcga tcggcatgaa ctcgccgttc aggtagatca
      541 tgcaaatgct ccagaaaatt tccgatagag caggcgtaag ctatgccagg ttcggataaa
      601 aacatttgcg atgccaacgt tttccagcgc tatgaccgtt tgttcgtcaa acggcttgcc
      661 atcaagagtc agctttagag tgccaacccg gtccccaatt cgtatcggtg ccatgagcgg
      721 ttgcatgctt tccatcgtgg ccttgagccg ctcggaaaac cccctcggca aggtgacaaa
      781 gtaatcgctg gccagcccgg agtgcagttc gttgctattg cctttccata ccggcagcga
      841 atgaacaggc tgccctgcac tgtagagcct tatcgtatcg aaagcctcga agccgtaatt
      901 caatagtttc tgtgcatcga ccgcccggcc attttccgag ccggcgtcga taaccacggc
      961 gatgaggcgc cgctcatttc gctgtgcaga ggccaccatg caatagccgg cgcttgttgt
     1021 atagccggtc ttcagtccgt caacgaaagg atcgcgtccg agtagccggt tgcggttgta
     1081 ctgcgttatg ttgttgtggc ggtactcgcg caccttgtac attgcaaagt actgtggaaa
     1141 ttcgtcgatg attgcacgcg ataatgttgc catgtctgcg gcagtggaaa aatgttgggc
     1201 gttcggcagg ccagtggcgt ttacaaagtg ggaatttttc agtcccatcc gggcggcttc
     1261 acggttcatc gcctccacga aggcttcttc gcttcccgag atgccctcgg ccaacgctat
     1321 tgccgcatcg ttgcccgatt gtacgatcat gccattgagc aacgcctcga ccggcacgct
     1381 ttcgttgatg gtcaggaaca tgcgcgaccc cggcgcacgc caggctttct gtgaaacggt
     1441 gaacggctgt accagcgtga tttgcttctg cctcaatgca tcgaatacca cgtacgcggt
     1501 cagcagcttg gtcagcgatg ccggttcgat gcgatcatcc ggattccggg ctgcgagcgt
     1561 ttgccggctc tgaaaatcga tcaggagata agcccttgaa ctcagcgacg gtactggagc
     1621 gctggagcca ggcaaacctt cggcgaacgc caaggagccc gacagaaagg cgattaaggc
     1681 aaggaatttg atcatcgtga agcggccaaa ttatacagga cgtatgtggc ctgaccgttg
     1741 ccgcgctggt tgacctctgg aacaatgcgc caagaccatc ggtacttcgt catgacaact
     1801 ttttttccca agcaaagcgt acagcgaatg ttgatactgc tctgcctgtg cgttggaact
     1861 cctgcgcaag ctgctgaact ggtaaagatc ctggacccgt gggcgcgtgc tacggtgccg
     1921 ggccagaaag tgggtggcgt ctacatggag attgtcgcac gagaaaattt gcgcctgacc
     1981 gggataagga ctacagcagc ggaatccgcg caagtgcacg tgatgaaaat ggaaaacggc
     2041 atgatgagga tgcgggccgt tccatttctg caattgcccg caggtaagcc agtcaagctc
     2101 acgccgggcg gctaccacat catgctgtac gatctgcggc gctcactggt atcggggcaa
     2161 gaactctcgc ttgaactgac cttcgaagat gcggcgaaac aacagtaccg ggtcccggtg
     2221 aaagtgatca ttcgtgaccg ggaagcggga gtgagttacg ggcacgaggc ccaatagtta
     2281 accgttcacg aagctaagtt ttgcaatcca gccttcatct acccagcggc gaagcgtgcc
     2341 ggcggcacgc gccgctgcct tgtcttcggg aaaccattgg cacagaccgc cgcagatttc
     2401 ctcaaagctt gcaccagcaa gcagtgcttt aagtgcccac gcttcatccg cctccagcga
     2461 tcgaaacaac ggcgtatcgt ccttgcgcca aatcacccag gtaactggct cagcggacac
     2521 ctgtactggt attcgtcctt tatcgccggt tgctttccag atggccacgg cattcgtggt
     2581 gagctccacg agatgcaagg ctgccggtgg caaaaagcgc atctccggcc ataagtgcgc
     2641 tgggactgtt gacacatctt cgaagctgac agactggctg tctgcagcgt cgaaagcgag
     2701 tcccaaagtc cattcaaaat gcgccagctc agcgagcagg ggattttccg aatacggaag
     2761 cgtcgatcca agaaattccg ccagctggcc gccaaaccag cgcacgttgc gaaaagtaga
     2821 aggataggcg tccaaatatt caagcgcagc agcgtcgaac gattcatcgc caagatattc
     2881 gcgcgaaacc gggtagtctt tgcccagcac ttcgagcagt cgaagccgat aggcatcacg
     2941 atatatgccg agcctgatgc ggcggaattc atcgcttgag ccctcgattt cgccttctat
     3001 agcgccgtca ccagccagaa tgccggcctg cagcagcgac tcgattgcgg caagctgctt
     3061 cacgcggcca ccttggcagg cagcgttctt tccgcaattt cgcgcgcatg gtttaattcg
     3121 ctcaccagtt cagcaagtgg ggggatctga tcgtcgcgtt caatcatggt ggcgacattg
     3181 ccaaaacggc gcagcgcgct cgcatacagt tcccataccg gatcgatgat gtcggcgtca
     3241 tgcgtatcga tgatatgcgt accgcaatgg cggtgtcccg ccaaatggat ttgccggact
     3301 cggtcaatag ggatgccgtc gaggtattca atcgcatcga agccgtgatt gaacgcgctg
     3361 acgtagacgt tgttgacatc aagcaatatg tgacaatcgg ctcggcgggc aatctcggcc
     3421 aggaaatgcc actcgctcat ctctgatccc cggaatgcca gataactgga aacgttctcc
     3481 agcagaatct ggcttcccag aaaatcctgc acttggcgaa cacgagcagc aatgtgcccc
     3541 actgcttctt ccgtatacgg aatcggtaac aaatcgtgca gattcttgcc cgcgatcccg
     3601 gtccagcaga ggtgatcaga aatccaacct ggctccgtgc gctgcatcag gtgtttgagc
     3661 gccttcaggt acccatagtc gagaggatcg gcactgccga tagaaagcga tacgccatgc
     3721 agggctaccg gatagtcagc gcgtatgcga tcgaggtagt gcagtggctt gcctccgggc
     3781 accagatagt tctcggttac tgcttcaagc cagtccacac gtggatggct ttcgaggatc
     3841 ggctggtagt gttcggtgcg caggccaagc ccgaaccctt gtacagatgc catagaaacc
     3901 ccaaggaaca atttaagaac gaagtcataa ccgtggaact aaccttgtcg cccgctgcgg
     3961 tctcatgcag cgagcgagta aaggcagcct gtcacggccg tgcaaggaat caacccgatc
     4021 cccgcacgca aacatacgta acaaaccaga tgttggatgc cgccgggtat cgggggagct
     4081 ggccttgaaa attcatgttc cggccccaaa agacgcgcgc aagtcgctag ccgattggct
     4141 aataacggct cttgaaagct tgccaagcgc gcctatatag cgtttggggc accggttgcc
     4201 caccctaatt taaaggagac acaaatcatg gccaatatga ctcgcta
//
LOCUS       DATMZQ010000448         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4355694, whole genome shotgun sequence.
ACCESSION   DATMZQ010000448 DATMZQ010000000
VERSION     DATMZQ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4355694"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VN496_14290"
     CDS             complement(<1..185)
                     /locus_tag="VN496_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXF18175.1"
                     /translation="MAKIAVFNQKGGVGKTTTSLNLTAALARRGYNPLSIDLDPQAHL
                     SYVCGSTVGNADDSIFS"
     gene            490..1485
                     /locus_tag="VN496_14295"
     CDS             490..1485
                     /locus_tag="VN496_14295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXF18176.1"
                     /translation="MSNDSYLAALTRMGLLQPGELPAITPLTGGVSSDIARVDLKTGP
                     ICIKRALAKLKVKMDWEAPVERNRYEIDWMRTAAGIEPLAVPKVLGEDRETGTFAMEF
                     LDEARYPVWKNQLRDGLIVDDTARQVGERISSIHAHTAGHIDVAQNFSTDHIFFPIRL
                     EPYLSATAKKHPDCAERLESLVRVTGATKKALVHGDVSPKNILAGLDGAVFLDAECAW
                     YGDPAFDLAFCLNHLLLKCVWKPQWQSRYLESFDTLAASYLANVSWESRDDIEERAAH
                     LLPGLLLGRIDGRSPVEYINTEAQREQVRRVARALLMRPVDSLAGVRESWQRRLV"
     gene            1520..2809
                     /gene="eno"
                     /locus_tag="VN496_14300"
     CDS             1520..2809
                     /gene="eno"
                     /locus_tag="VN496_14300"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXF18177.1"
                     /translation="MTDTTITKIHGRQVWDSRGRPTVEVEVHVSGGAVGRAIAPAGAS
                     RGANEAVDLRDGGQAFGGMGVTRAVAHVNNEISGLLLGRNALRQAEIDDELIACDGTP
                     NKSRLGGNAMIATSMAVLHAVAGAHRVPLYAYLAQGKSVRMPLPEIQIFGGGAHAGRR
                     VDIQDFMVMALAAHSFSEAAAITAEIYRAAGELMHDAGKLQGVADEGGFWPVFDCNED
                     ALTMLMRAIERAGYRPGTDVGISLDIAASEFGRDGRYRIEGRDLDSDGLCEMLLGWLD
                     RYPIVSIEDPLAEDDAEGMKKFTAAAGHKVQIIGDDFLVTNANRVRAAADAGACNAVL
                     IKPNQAGTVTETKAALDAAKRVGWGTIVSARSGETEDVTIVHLAAGWDAGQLKVGSFA
                     RSERMSKWNEALRIEESLAGKTSFAGRDALPSKHVNR"
BASE COUNT          606 a          833 c          915 g          560 t
ORIGIN      
        1 aaactgaaaa ttgaatcatc ggcattgccc acggtactgc cgcaaacgta tgacaaatgg
       61 gcttgcggat cgaggtcaat ggacaaaggg ttgtagcccc gcctggccag cgcggccgtg
      121 aggttcagtg aagtcgtcgt cttgccgact ccgccctttt gattgaaaac cgcgatcttg
      181 gccatggcag accgccgaaa cgcgaattgc cgcacggctg aagaagtcac aatactgatg
      241 gaatcagttg gttgtcaatg aactttcaag tgactttgat ttgtcgtcgg catttcgata
      301 ctgtgcgcgc tcgcggatgc tgcggatccg tgacctattg cggcgcccat gcgcgctcgt
      361 tcttacagcg agccggcgtg ttcagaaggt gtgataaagt ccagtcccgc tgtcgtgccc
      421 gagagatgcg cttcaatcac atgcgcgaaa ccgcagcgtg cagcgatgtc gcggtctttc
      481 cagataataa tgagcaacga ctcctacctt gctgcgctca cgcgcatggg gttgcttcag
      541 cccggtgaac ttcccgccat tacgcctctc accggcggtg tgtcctcgga tatcgcgcgg
      601 gttgacctga aaaccggacc catctgtatc aagcgagcgc ttgcaaagct gaaggtcaaa
      661 atggactggg aagcgccggt cgaacgcaac cgctatgaga tcgactggat gcgcactgcg
      721 gccggcattg aaccgcttgc cgtaccaaaa gtgctcggag aggatcgcga aaccggcacg
      781 tttgccatgg aattcctgga tgaggcgcgc tatccggtct ggaagaacca gctgcgtgac
      841 ggcctgatag tcgacgacac cgcccgccag gttggtgagc gcatttcatc gattcacgcg
      901 cacacggcgg gccacatcga tgtcgcgcaa aatttttcta ctgaccatat cttttttccg
      961 atcaggctcg agccttatct ttcggccact gccaagaagc atcccgactg cgctgaacgg
     1021 ctggagagcc tggtgcgagt taccggcgcc acgaaaaaag cgctcgtgca tggcgatgtc
     1081 agcccgaaaa acattctggc cggccttgac ggagcagtgt tcctggacgc cgagtgcgcg
     1141 tggtatggcg atccagcatt cgatctcgcg ttttgcctga atcatctgct gctgaagtgc
     1201 gtctggaaac cgcagtggca gtcgcgttat ctggaaagct ttgacacgct ggcggcaagc
     1261 taccttgcca acgtcagctg ggaatcgcga gacgacatcg aagaacgtgc cgcgcatctg
     1321 ttgcccggcc tgctgcttgg tcgcatcgac ggcaggtcgc cggtggagta catcaacacc
     1381 gaggcacagc gcgagcaggt gcgccgcgta gcacgggcgc tgctgatgcg gccggttgac
     1441 agccttgctg gcgtacgcga gtcctggcaa cgccggctcg tgtgagaacc gattcagcat
     1501 tacagaagcg aaagtgttca tgaccgacac cacgattacg aaaatccatg gccggcaagt
     1561 ttgggattcg cgcggccggc ccacggttga agtcgaagtg catgtctcgg gcggggcagt
     1621 cggacgcgcg atcgcacccg ccggcgcatc acgtggagca aatgaagcgg tcgacttgcg
     1681 cgatggcggc caagccttcg gcgggatggg cgtgacgaga gcggtcgcgc acgtcaacaa
     1741 cgaaatatcc ggcttgctgc tcgggcgcaa tgcgcttcgc caggccgaga tcgacgacga
     1801 actgattgcc tgcgacggca cgccaaacaa gtccagactt ggcggcaacg ccatgattgc
     1861 cacgtcgatg gctgttctgc acgccgtggc cggcgcgcat cgcgtgccgc tgtacgctta
     1921 cctggcacag ggcaagagtg tgcgcatgcc gcttcccgag atacagatct ttggcggggg
     1981 tgcgcatgct ggacggcgtg tcgacatcca ggatttcatg gtgatggcgc tggccgcgca
     2041 cagtttcagc gaagcggccg ccatcacggc cgaaatttat cgggctgccg gcgagttgat
     2101 gcatgacgcg ggcaagctgc aaggcgtcgc tgacgagggc ggtttctggc ccgtgttcga
     2161 ctgcaacgag gacgccctca ccatgcttat gcgtgcgatc gagcgggccg gttaccgtcc
     2221 gggcacggac gtgggcatat cgctcgacat cgcagcatca gaattcgggc gggacggccg
     2281 ctatcggatc gaagggcgtg acctggacag cgacggcttg tgcgaaatgt tgctcgggtg
     2341 gctggaccgt taccccatcg tctcgatcga agacccgctg gcggaagacg acgccgaagg
     2401 catgaaaaaa ttcaccgccg cagcggggca caaggtgcag atcattggcg acgatttttt
     2461 ggtcaccaat gccaatcggg taagggcggc agctgatgct ggtgcctgca acgccgtgct
     2521 tatcaagccc aaccaggccg gcacggtaac ggagaccaag gcggcgctgg atgcggcaaa
     2581 gcgtgtcggc tggggcacca tcgtttcggc gcgctccggt gagaccgagg acgtgactat
     2641 cgtgcatctc gcggcgggtt gggacgccgg acaactgaag gtcggttcgt ttgcgcgctc
     2701 cgaacgcatg tctaaatgga acgaggcgct acgcattgag gagtcgctcg ccggcaaaac
     2761 cagcttcgcc ggacgcgacg cgctgccaag caagcacgtg aataggtgaa tgtcgtaacg
     2821 atgctgaata acgaaaaagc aagactatta ctctcatcac actgttcgcg acagagacgg
     2881 cgctccccct gtccacgttt caggcaatga cgac
//
LOCUS       DATMZQ010000449         4664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_904646, whole genome shotgun sequence.
ACCESSION   DATMZQ010000449 DATMZQ010000000
VERSION     DATMZQ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904646"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VN496_14305"
     CDS             <1..379
                     /locus_tag="VN496_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18178.1"
                     /translation="AVESRAPEEIVLWRIPVGEGAIARIDDRTLTLPQGIIGFEYLQI
                     DHDLLGYHQLIPLAFLGAERLELTHSAPEKWFLETLWKRAQGYSERGLSVRSRVEQFS
                     VIPNQQYQLVERDKKVFFRPASQ"
     gene            complement(405..1142)
                     /locus_tag="VN496_14310"
     CDS             complement(405..1142)
                     /locus_tag="VN496_14310"
                     /inference="COORDINATES: protein motif:HMM:NF006118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18179.1"
                     /translation="MKIAGSVALVTGSNRGIGRAYVKTLFEMDAKKIYAAARDISGVS
                     DLVKTYPGKVVALTLDVTNEAAVKAVAAKCADVSLLINNAGANGSSGALAAGSIEAAY
                     REMMVNYFGVLFLSRAFGPVLKLNGGGAIINMLSLVSRINIPALGTYSASKAAAWSLT
                     QAVRAELAKQETLVVGIFPGAVDTDMARGIDLPKLAPDDVAAIALKAVESGMEDVDVG
                     DMAQGVAAGLLANPKKVEKEFAAYLPG"
     gene            1579..3156
                     /locus_tag="VN496_14315"
     CDS             1579..3156
                     /locus_tag="VN496_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18180.1"
                     /translation="MAAESASEGHLPSFAELQDAGAIIGEVRIINNNIFNLDDPKENN
                     FLFRLANNLHIVTRPEVIRRSLLFSSGERLSIQKIEESERVLRRNRYLYDVEIRPAAY
                     HEGIVDIEVQTRDTWTIQPGLQYSRSGGSNSTALTLRENNLLGTGTSIGYAKTSDVDR
                     NGNAFSLAQHNLFGSWTSIDLTLAKYDDGDHRSFEFGRPFYALDTRWATGVSALHDER
                     IESVYVNGDIVGQYRHIKDASEVFGGWSNGLVDGWTRRYSVGLNYLDDVYPSDPTLAA
                     PPAVPPDQRLVSPFVRYEIIQDDFEKVKNRDKIQRAEFFAIGFNSTIQVGRALTSLGS
                     TRDLWLYSATATRGMHLERGNDLLGSASVGGQYGDGHGERQTLGGSVRYFVPHFKRAV
                     FYVAASGDTIRNPEPQDVLLLGGENGLRGYPLRYQSGEHRALLTIEERVYTDWYPVRL
                     FRVGGAVFYDVGRAWGGPTQNPVNPGWLSDVGFGLRILNDRAAFGNVLHIDLAFPLNP
                     DPNIKSVQFLVRSYLNF"
     gene            3297..4538
                     /locus_tag="VN496_14320"
     CDS             3297..4538
                     /locus_tag="VN496_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXF18181.1"
                     /translation="MAGQWIDIKTAQEGTFRGYLALPASGKGPGIVLCQEIFGINAYI
                     REVADYYAEEGYVVLAPDLFWRLEKDVDLGYSEAEWKKAFDLLQRMDVDKAMEDVTAA
                     VRTLRTRVEVSGKVGALGFCLGGRLAYLAAARSGVDCAVGYYGVTIEKYLNEAARIKV
                     PMVLHFASEDKYAPPEAVEKIRNAFSGRKDVEIYSYPGVDHAFARTGGMHYDRPASMM
                     AHSRSIALFRKVMGPAYDLSALWEQHTMHEFGTRDVDATMKTMVAQPYVNHVPTMTGG
                     VGHDHLKRFYKYHFIPTTPKDTRLVPISRTIGADRLVDEMLFCFTHDIEIDWMLPGVA
                     PTGKYVEVPLVAIVNFRGDKLYHEHIYWDQASVLVQIGLLDPKKSGLPVAGAETARKL
                     VDENVPSNRLMKNWSSSEGKR"
BASE COUNT          990 a         1328 c         1355 g          991 t
ORIGIN      
        1 cgcggtcgaa tcccgggcgc cggaagagat cgtcttgtgg cgcatcccgg tcggcgaagg
       61 cgccattgca cgcatcgatg atcgtacgct gacgttgcca caaggcatca tcggcttcga
      121 atatctgcag atcgaccacg atctgctggg ttatcaccaa ctgattcccc tcgcgtttct
      181 gggcgccgag cgtttggagt tgacccattc cgcaccggaa aagtggttcc tcgagacttt
      241 gtggaaacgc gcacaaggtt attctgagcg cggcctttcg gtacgtagcc gtgtggaaca
      301 atttagtgtc attccgaacc agcaatacca actggtcgag cgcgacaaga aagtcttttt
      361 ccgtccggcc tcgcaataac atcgcaaaga ccggcgcgga gtgttcaacc gggcaggtag
      421 gcagcaaact ccttttccac ttttttggga ttggccagca gcccggcggc tacgccttgc
      481 gccatatcgc ccacatcgac gtcttccatg ccggattcca cggctttgag cgcaattgcg
      541 gcaacgtcgt ccggcgcaag tttgggcagg tcaatgccgc gtgccatgtc cgtatccaca
      601 gcgcccggga aaatgccgac aaccaaggtc tcttgttttg ccagttccgc ccgcacggct
      661 tgcgtaaggc tccaggctgc ggctttcgat gcggagtagg taccgagcgc aggaatgttg
      721 atgcgcgaca cgagcgaaag catgttgatg atggcaccgc ccccattgag tttcagaacc
      781 ggaccgaacg cacgcgacag aaatagcacg ccgaagtaat tcaccatcat ctcgcggtaa
      841 gccgcctcga tcgagccagc cgccaatgcg ccgctagacc cgttggcgcc ggcgttattg
      901 atcagcagcg agacatccgc acatttcgcg gcaacagctt tgacggctgc ttcattggtg
      961 acgtcaagcg taagtgcaac gaccttgccc gggtacgtct taaccagatc gcttacgccg
     1021 ctaatatcgc gggcggcggc atagattttt tttgcatcca tctcgaacag cgttttgacg
     1081 tatgcgcggc ctatgcctcg attcgaaccg gttaccagcg cgactgaccc tgcaatcttc
     1141 atgttgcctc tctccttctg tttggaattt gcagctagtg actattgcag ctgctctagt
     1201 tttcgcctgt tcgccaaggt gggcatcgaa accgggcaac gcatggcatt gcaaggcgca
     1261 agtgtaagcc tggacaaggc agacggaaag ccttgcgcca ccgccgcgat gtcaatcgtt
     1321 gcgtgcgcgc attgtcgcac cgggcgcggt agcgttcact ttcggcatgg aatgccgatt
     1381 tgctattcca acatgattca atgttccggc acactgacag catgggagag cgcgtaccag
     1441 caccgggaca gacaggggcg acatctgctt aattgctatc ccacaccacg gctgttacag
     1501 cgagattgcg ttgctttgcg aaaacgcgcg ctgcgatggt tgatttgcgc gaccgcctgg
     1561 tgcctttgca cgcatgcgat ggctgccgaa tcggcttcgg aagggcactt gccttcgttt
     1621 gcggaattgc aggatgccgg cgccatcatt ggcgaagtgc gcatcatcaa caataacatt
     1681 ttcaatctgg acgatcccaa ggaaaacaat ttcctgttcc ggctcgccaa caatctgcat
     1741 atcgtcaccc gcccagaggt gattcgccgg tcattactgt tcagtagcgg cgagcgcctg
     1801 tccatacaga aaatagagga gtcggaacga gtgctgagaa ggaaccgcta tctctacgac
     1861 gtagagattc gacccgctgc gtatcacgaa ggcatcgtcg acatcgaagt ccagacgcgc
     1921 gacacctgga caattcagcc cggtttgcaa tacagccgct ccggcggttc gaattcgacc
     1981 gctcttacgc tcagggaaaa caatcttctg ggcaccggta cttcgatcgg ctatgcgaaa
     2041 acttcggatg tcgaccgcaa cggcaacgcc ttttccctgg cgcagcacaa cctgttcggc
     2101 agctggacca gcatcgacct gacattggcc aaatatgatg atggcgacca tcggtcgttc
     2161 gagttcggcc ggcctttcta cgctctcgat acgcgctggg ccacgggcgt gtcagctttg
     2221 catgacgagc gcatcgaatc agtctatgtc aatggcgata ttgtcggcca atatcgccac
     2281 atcaaggatg ccagcgaagt gttcggaggc tggtcaaacg gcctggttga tggctggacc
     2341 cggcgatatt cggtcggcct caactatctg gatgacgtct acccgagtga tcccacactg
     2401 gcagctcctc ctgccgtgcc accggaccag aggctggtgt cgcccttcgt gcgctacgag
     2461 atcattcagg acgacttcga aaaggtgaag aaccgggaca aaatccagcg tgcggaattc
     2521 tttgctattg gattcaattc cacgatacaa gtgggccgcg cgctgaccag cctgggttcc
     2581 acccgcgacc tctggctcta ttcagccacg gcgactcgcg ggatgcatct ggagcgcggc
     2641 aatgacttgc tcggttcagc aagcgtcggg ggtcagtacg gagacgggca cggagaacga
     2701 caaacgttgg gcggctcggt tcggtacttc gtgcctcact tcaagcgcgc cgttttctat
     2761 gtcgccgcgt cgggcgatac gatacgcaat cccgaaccgc aggatgtctt gttgctgggc
     2821 ggtgaaaatg gtttgcgcgg ctatcccctg cgctaccaaa gcggcgagca ccgggcactg
     2881 ctcaccatcg aagaacgcgt ctacacggat tggtatccgg ttcgattgtt tcgcgtcggt
     2941 ggcgcggtat tctatgacgt cggccgggcg tggggtggac cgacccagaa tccggtgaac
     3001 ccgggctggc tcagcgatgt gggtttcggc ttgcgcatcc tcaatgaccg cgccgcgttt
     3061 ggcaatgtgc tgcatatcga cctcgccttt ccactcaatc ccgatccgaa catcaagtca
     3121 gtgcaattcc tggtcaggag ctatctgaat ttctagcagt gttgactcgg cacatgtttt
     3181 cgccaggcaa tacacccgtg gtcgtttgac agcgcaagcc tgcacgcggt aaaaggtcag
     3241 ccagtttttg cgtgcttcaa tgcgccgcaa cagtcaaatt acatccgggg agcgacatgg
     3301 caggacagtg gatcgatatc aagacagcgc aggaaggtac attcagagga tatcttgctc
     3361 tgcccgcatc aggcaaaggc cccggcatcg tgctgtgcca ggagattttc ggcatcaatg
     3421 cgtacatccg cgaggtcgcc gattactacg ccgaggaagg gtacgtggtg ctcgcaccgg
     3481 atctgttctg gcggttggag aaggacgtcg atctcgggta ctcggaagct gagtggaaaa
     3541 aagcgttcga tcttctgcag cgcatggacg tcgacaaggc catggaagac gtcacggctg
     3601 cagtacgcac cttgcggacg cgggtagaag tgtccggcaa ggtcggcgcc ctcggattct
     3661 gtctcggagg caggctcgcc tatctggcgg ccgcgcgctc cggtgtggat tgcgcagtcg
     3721 gctactatgg cgtaacgatc gagaagtatc tcaacgaagc ggccagaatc aaggttccga
     3781 tggtgttgca cttcgccagc gaagacaaat acgcgccacc ggaagcagtg gaaaaaatca
     3841 ggaatgcgtt ttcaggccgc aaggatgttg agatttatag ctacccgggt gtcgaccatg
     3901 cattcgcgcg cacgggcggc atgcactatg accggccagc ttccatgatg gcgcactcac
     3961 gttcaatcgc attattccgc aaggtcatgg ggcccgccta cgacctgtcc gcgctgtggg
     4021 aacagcacac aatgcatgaa ttcggcacac gcgatgtcga cgcaacgatg aagaccatgg
     4081 tggcgcagcc ctacgtgaat cacgtaccca ccatgacggg cggcgttggc cacgatcatc
     4141 tgaagcgctt ctacaagtac cacttcattc cgacgacgcc gaaagacacc cggctggtgc
     4201 caatctcacg cactatcggg gcagatcgcc tggtcgacga aatgctgttc tgtttcacgc
     4261 acgacatcga aattgattgg atgctgccgg gtgtcgcgcc gaccggcaag tatgtcgagg
     4321 tcccgctcgt ggcaatcgtg aactttcgtg gcgacaagct atatcacgaa catatctact
     4381 gggaccaggc ttccgtgctg gtgcagattg gactgctcga cccgaagaag agtgggctgc
     4441 cggttgccgg cgcggagacc gcgcgcaagc tggtggacga gaatgttccc tccaataggt
     4501 tgatgaagaa ttggagctcg agcgagggca agcgctgaag gttcgaaggc gcgaaccaag
     4561 taccgccggg cagccggcgt agagtatcag gacttcgtgc tactgctttt cggcaggcag
     4621 ttgctgtagc gcgcggaaca tctcctgttg tatgacgggc atca
//
LOCUS       DATMZQ010000450         5852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3671301, whole genome shotgun sequence.
ACCESSION   DATMZQ010000450 DATMZQ010000000
VERSION     DATMZQ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671301"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VN496_14325"
     CDS             complement(<1..829)
                     /locus_tag="VN496_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXF18182.1"
                     /translation="MKITDLKIHVLKSQLTEPFAFSQGWVKQRSATLVEVFTDGGITG
                     WGEAFAQGLEPPEIAAAVIENALKPLVIGADPLDMEVLWHRMYHITRDYGRKGSVIAG
                     ISAIDIALWDIAGKFHGLPVYKLFGGAFRTRVQPYATGFYRIKGQGEARRLGEEALRH
                     FEAGFSLMKIKLGYGVEDDVACMKEIGRAIQGKGITLMVDTNHAYGRAEALKLGRALE
                     EYDLRWYEEPVVPEDVPGYVEMRSKLSMPIAGGENEHTLYGFRELFGESAVDIAQPDL
                     "
     gene            950..1390
                     /locus_tag="VN496_14330"
     CDS             950..1390
                     /locus_tag="VN496_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXF18183.1"
                     /translation="MYRKILLPTDGSALCDSAAQKGIDFAKFAGASVVAFHAIPATSY
                     LIYTEAGPSDVLAEQFEREAKMRGQQLVDSLEHCARQAGVPCETLLATNDHPWEGIIA
                     AANEKGCDLIFMASHGRRGFTAMLLGSETARVLTHTKIPVMVYR"
     gene            complement(1442..1762)
                     /locus_tag="VN496_14335"
     CDS             complement(1442..1762)
                     /locus_tag="VN496_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18184.1"
                     /translation="MKKSILLLALLWSGLVLAEDDGDDLLVLMEKPATAPIALEHIDD
                     VRMRWDGSVFCIAKRNSSAPPDPQRAAFTAVKTYLEAYPAERYRPRRYLIIQGLRAAY
                     PCQQ"
     gene            complement(1802..1877)
                     /locus_tag="VN496_14340"
     tRNA            complement(1802..1877)
                     /locus_tag="VN496_14340"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1842..1844),aa:Thr,seq:cgt)
     gene            complement(1932..2714)
                     /locus_tag="VN496_14345"
     CDS             complement(1932..2714)
                     /locus_tag="VN496_14345"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXF18185.1"
                     /translation="MSYENIIVETRGKVGLVTLNRPKALNALSPELMHEIAAALDTFE
                     ADENIGCMVLTGNEKAFAAGADIKAMKQKSYMDVYKEDFISAQWERVTHCRKPVIAAV
                     AGYALGGGCELAMMCDFIIAADNAKFGQPEINLGIIPGAGGTQRLPRFVGKSKAMEMV
                     LLGQARLMDAAEAERVGLVSRVVPLAQLMDEVLKAANKICELSLPVVMMAKESVNRAY
                     ETTLAEGVRFERRLFHSAFAIEDQKEGMAAFIEKRKPVFKNR"
     gene            2792..3580
                     /gene="paaG"
                     /locus_tag="VN496_14350"
     CDS             2792..3580
                     /gene="paaG"
                     /locus_tag="VN496_14350"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HXF18186.1"
                     /translation="MKFDAILLDIAEGIATITLNRPDKLNAFTSEMHADLKQAMQIIR
                     DDPSVRVLMLTGAGRGFCAGQDLSERTMSKEQTGDVGNSLEKNYNPLLKQLRALPYPV
                     VCAVNGVAAGAGCNLALACDIVIAARSASFIQAFSRIGLIPDAGGTYTLPRLVGTARA
                     MAAAMLAEKIDAERAMQWGMIWNCVDDAQLMSEAKALARQLAGQATRALGLTKRAIYQ
                     SANHSFEQQLDLERDLQREAAKSEDFREGVTAFKEKRAARFTGR"
     gene            complement(3590..4009)
                     /locus_tag="VN496_14355"
     CDS             complement(3590..4009)
                     /locus_tag="VN496_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXF18187.1"
                     /translation="MQPGQYTRDNLAEKLRGHGINPTHQRIEIAYALFSRCEHLSADQ
                     IMAIVNERQSETSKATVYNTLNLFLEKKLIREVIVDPNKVFYDPNTAAHYHMYDVQSG
                     QLTDIDATDIRITGLPSLPAGMVTEGMDIIIRVRPTD"
     gene            complement(4062..4730)
                     /locus_tag="VN496_14360"
     CDS             complement(4062..4730)
                     /locus_tag="VN496_14360"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXF18188.1"
                     /translation="MHALLMLSLSKNSLNGSVFTQPVHEPLRVRIEKLSESQHASPIL
                     INKKKAVVHKETAATPPVTPAVPEQVQTSLPQPGVSVAETLYLRPIPSRANSALLETG
                     EFHRSSELTEQPQTIRMQIPEYPQPAKEQSLSGWVLVMLLVDENGKVLETAAVDSSEV
                     FGEFRQDIAQEMRESSFIPGKLDGQAVKTLVFAMVGFDSKALSGIDVPSASTPQLTDQ
                     PKTH"
     gene            5061..5486
                     /locus_tag="VN496_14365"
     CDS             5061..5486
                     /locus_tag="VN496_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HXF18189.1"
                     /translation="MKKPGGFTLIEILIAVAIVGILAAIALPSYNQYVARGKLTEAFS
                     NLLAIRTQSEQWFQDNRTYAGMPCSTTNARYFTYACTGLSATTYTITATGVAATDVEG
                     LGFSIDQSNARSSVATGAAATKGWVGNAACWIVRKNGSC"
     gene            5486..>5852
                     /locus_tag="VN496_14370"
     CDS             5486..>5852
                     /locus_tag="VN496_14370"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXF18190.1"
                     /translation="MRARSAAGMTLIELLIGFVIVGVLLALGVPSFNAWLQNLQLRNA
                     ADSIFNGLQLARASAVQRNKSVTFTMAGPGSDWTVTIDTPSALETATVQTRSATEGTP
                     NAVIATTAPAISFNGLGATN"
BASE COUNT         1254 a         1691 c         1622 g         1285 t
ORIGIN      
        1 caaggtcagg ttgtgcaatg tccacggcgc tctccccgaa caattcacgg aagccgtaca
       61 aggtatgttc gttttcccca ccggcgatgg gcatcgatag cttcgagcgc atttcgacgt
      121 aacctggcac atcttcagga acaaccggct cttcgtacca gcgcaggtcg tattcctcca
      181 gcgcgcgccc cagcttcagt gcctctgcgc gcccgtaggc gtgattggta tcgaccatca
      241 gcgtgatccc tttgccctgg atagcgcggc ctatttcttt catgcacgcc acgtcatctt
      301 ctacaccgta cccgagcttt attttcatta gcgagaaacc cgcttcgaag tgccggagcg
      361 cttcttcgcc cagtcggcgt gcctcgccct gccctttgat gcgataaaaa ccggtcgcgt
      421 agggttgtac gcgcgtacga aacgcgccac cgaacagctt gtataccggt aacccatgga
      481 attttcccgc gatgtcccac aacgcaatat cgatggcgct gatgccggct attactgaac
      541 ccttgcgccc gtaatcgcgg gtgatgtggt acatgcggtg ccaaagcact tccatatcca
      601 gcggatcggc gccgatgacc aaaggcttca atgcattctc gataacagca gcagcaattt
      661 ctggtggctc cagtccctgc gcgaaggctt cgccccagcc ggtgatgccg ccatccgtga
      721 acacctcgac gagagtggcc gaacgctgct tcacccagcc ttgcgaaaac gcgaacggtt
      781 cggtcagctg gctttttagg acgtggatct tcagatcggt tattttcact gacggtgtcc
      841 ccagattatg tgcagcgatt cccattgatt tggcgggcgc gataattaac cataacgtcc
      901 cggaacggtc tataactgta aggcttgtcc tgccaaacgg agggtcgaaa tgtatcggaa
      961 aatcctgcta ccgacagacg gctcggcatt atgtgattcc gccgcgcaaa aaggcatcga
     1021 ttttgcgaag ttcgccggcg ctagcgtggt ggcctttcat gccatccccg ccacgtctta
     1081 cctgatctat acggaagccg ggccgagcga cgtccttgcg gagcaattcg agcgcgaagc
     1141 caaaatgcgc ggccagcaac tggtcgacag ccttgaacat tgtgcgcgcc aggcgggagt
     1201 gccctgcgaa acgttactcg caactaatga tcacccttgg gaaggcatca tcgcggccgc
     1261 caacgaaaag ggttgcgatc ttattttcat ggcgtctcac gggcgccgcg gcttcaccgc
     1321 gatgctgctg ggcagcgaaa cggcgcgggt gctgacccac accaaaatcc ccgtgatggt
     1381 gtatcgctaa ggtcgcttgc agaccggcat tcgattcgcg ctcttgggga ttacggagaa
     1441 gttattgttg acacgggtac gcggcacgta gcccctggat aatcaggtaa cggcgcgggc
     1501 gatagcgttc cgcgggatag gcctccaggt aggttttcac ggcggtgaac gccgcgcgct
     1561 gcggatcggg cggcgcactg gaattgcgtt tggcgatgca gaacacactg ccgtcccagc
     1621 gcatcctgac atcgtcgatg tgctcaagcg caattggtgc tgtcgcaggc ttctccatca
     1681 ataccagcag atcgtcgcca tcgtcttcgg cgagcacgag cccggaccac agcagcgcaa
     1741 gcagtaaaat actttttttc atgcaggact gcgcttgcta atgcgggatg tgacgtgttg
     1801 atggtgccgc tggccggagt cgaactggcg accttcgcat tacgaatgcg ctgctctacc
     1861 aactgagcta cagcggcgtg ggcgcgcatt atacggctgc gccgaccctg cgggcaaacg
     1921 cgatccgctg cttagcgatt cttgaaaacg ggcttgcgct tttctatgaa agcggccatt
     1981 ccttccttct ggtcctcgat cgcaaaggcc gaatgaaaca ggcgtcgctc gaagcgcacg
     2041 ccttcggcga gtgtggtttc gtacgcgcgg ttgaccgatt ctttggccat catcacaacc
     2101 ggcagcgaca attcgcaaat tttgttcgcc gccttcaata cctcatccat cagttgtgcg
     2161 agcggcacga cccggctgac aaggcctacc cgctcggctt ccgccgcatc catgagtcgc
     2221 gcctgcccaa gcagcaccat ttccatggct tttgattttc cgacgaaccg cggcagccgc
     2281 tgggtgccac ccgcgccggg aataatcccg aggttgattt cgggctggcc gaatttggca
     2341 ttgtccgccg caataataaa atcgcacatc atggccaact cgcatcctcc gccgagcgca
     2401 tacccggcga cggcagcgat caccggcttg cggcagtgag tgacgcgctc ccactgggcc
     2461 gaaatgaaat cttccttgta cacgtccata tagcttttct gcttcatcgc tttgatatcc
     2521 gcaccggccg caaacgcctt ctcgttgccg gtcaagacca tgcagccgat gttctcgtct
     2581 gcttcgaatg tatccagcgc tgcagcaatc tcatgcatga gttccggaga aagggcgttc
     2641 agcgccttgg gccggttcag cgtcaccaat ccgactttgc cacgggtttc gacgatgata
     2701 ttttcatacg acatgactgg tccttgttgg tgagttttat aattccgccc acgcaccgca
     2761 cccgcggacg aaaacgagaa agggaaaagc agtgaaattc gatgccattt tattggatat
     2821 cgccgaggga atcgctacga tcacattgaa ccggccggat aagctaaacg ctttcacgag
     2881 tgaaatgcat gcggatctca agcaggccat gcaaattatc cgggatgatc ccagcgtgcg
     2941 cgtgctaatg ctgaccggtg ctggtcgcgg attttgcgcg ggacaggacc tttccgagcg
     3001 cacgatgagc aaagaacaaa caggcgacgt tggcaattcg ctggaaaaaa attacaaccc
     3061 attgctgaag caactgcgcg ctctgcccta tccggtggtc tgcgccgtca acggtgtagc
     3121 ggcaggcgct ggctgtaacc ttgcattggc ttgcgacatc gtcatcgctg ccagatcggc
     3181 aagcttcatt caagcattca gccggatcgg actgatacca gatgccggcg gcacttatac
     3241 attgccgcgc ctggtgggca cagcgcgcgc gatggcagct gccatgcttg cagagaaaat
     3301 agatgcggaa cgggcgatgc aatggggaat gatctggaac tgcgtggacg atgcacaact
     3361 gatgagtgaa gcaaaagccc tggcgcggca actcgccggg caagcgacca gggcgctggg
     3421 actgaccaag cgcgcgatct accaatccgc caatcattcg ttcgagcagc agttggatct
     3481 cgaacgtgat ttgcagcgag aagccgcgaa aagcgaagat tttcgtgagg gcgtaactgc
     3541 gttcaaggaa aaacgagcgg ccagattcac cggccgttga ctattgcgct taatcggtgg
     3601 gccgaacccg gatgatgata tccatgccct cggtcaccat gccggccggc agagaaggca
     3661 aacccgtaat tcggatgtcc gtcgcatcga tgtcggtgag ctgacctgac tgcacgtcat
     3721 acatatgata gtgcgcagcg gtattgggat cgtaaaatac tttgttggga tcgacgatca
     3781 cttcgcggat gagcttcttt tcgaggaata gattaagcgt gttgtagacc gtggctttcg
     3841 atgtctcgga ctggcgctcg ttaacgatag ccatgatctg atcggccgaa agatgctcgc
     3901 agcgcgaaaa caacgcatag gcgatttcaa tgcgctggtg cgtcgggttg attccgtgtc
     3961 cgcgcaactt ttccgccaga ttatcccggg tgtactgacc tggttgcatg gcgaaattat
     4021 aggataggcc ttcggccact gtctaacccg tgttcgcagc gtcagtgcgt tttaggttga
     4081 tcagttaatt gcggcgttga ggcagacgga acgtcaatgc ctgacaaggc cttcgaatcg
     4141 aatccaacca tggcgaaaac cagcgtcttg accgcctggc cgtcgagctt gcccggaata
     4201 aaggaagact ctcgcatttc ctgagcaata tcttggcgga attcaccgaa cacctcgctg
     4261 gagtcgaccg cagccgtttc cagcaccttc ccgttttcgt ccaccagcaa catgacgagg
     4321 acccagccag acaagctttg ctcctttgcg ggctgcggat actccggaat ttgcatgcgg
     4381 attgtttgag gttgttcggt gagctctgac gagcgatgaa attcaccagt ttccagcagt
     4441 gcgctgttcg ctcgactcgg tatcggacgc aaatataagg tctcggcaac ggagactcct
     4501 ggctgaggca acgaggtctg cacctgttcc ggaacggctg gggtcactgg cggggttgca
     4561 gccgtctctt tatggaccac cgcctttttc ttgttgatca agatcggcga tgcgtgttgc
     4621 gattcagata atttttcaat gcggacacgc agcggctcgt gtacaggctg cgtgaacacg
     4681 ctgccattga gcgaattttt cgatagcgac aacatcagca gcgcgtgcaa taacgcggat
     4741 acaacaagcg ccatgcccag ccgccgatcc ggtgcaacag gccaataacc cagaatgttt
     4801 gtgctcgcca tcgctgcttt ttacttacat cagcgattta aatcaatccc cgagcgctcg
     4861 atctgctctc tgccgcgttc agtatcttta gcgcgaaaat tgttcagtac tgccgcaatt
     4921 tgtccgctca cagaaatgcc aatggcgtgc gcaattcgcg ttgaatgccg ttcattctgc
     4981 caaagcgacc tcttgtcgga agccgatggc ataggcattc gccggtcgct actctgcgtc
     5041 ctacctaaca ggaggaacgc atgaaaaagc cgggcggatt cacgttgatt gaaatcttga
     5101 tcgctgttgc catcgttgga attctggccg cgattgcact gccgtcctat aaccaatacg
     5161 tggcccgcgg caagctgacc gaggcttttt caaacctgct cgcaattcgc acgcagtcgg
     5221 aacaatggtt tcaggacaac cgcacgtatg ccggtatgcc ctgctcgacg acgaacgcga
     5281 gatatttcac ttacgcctgc actggcctgt ccgccactac ttatacgatc accgcaaccg
     5341 gcgttgccgc aacggacgtc gagggactgg gcttttccat agaccaaagc aatgcccgct
     5401 cgagcgttgc caccggagcg gcggctacca aaggatgggt cggcaacgcc gcttgctgga
     5461 tcgtcagaaa gaacgggagc tgctgatgcg cgctcgttca gcggccggca tgacactcat
     5521 cgagttgtta atcggcttcg tcattgtcgg cgttctgctg gccctcggcg tgccgagctt
     5581 caatgcctgg ctgcaaaacc tgcagcttcg caatgcagcc gactcgattt tcaacggact
     5641 gcagttggcc cgcgccagtg cggtacagcg taataaatca gtgacgttca ccatggctgg
     5701 cccgggttct gactggacgg tgaccatcga tacgccatcc gcgctcgaga ctgccacagt
     5761 gcaaacgcgc agcgctacag aaggcacccc aaacgcagtc atcgcgacta ccgcgccggc
     5821 catcagcttc aacgggctcg gggcgaccaa tc
//
LOCUS       DATMZQ010000451         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4362883, whole genome shotgun sequence.
ACCESSION   DATMZQ010000451 DATMZQ010000000
VERSION     DATMZQ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4362883"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VN496_14375"
     CDS             complement(<1..384)
                     /locus_tag="VN496_14375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXF18191.1"
                     /translation="MMKLKCLAAVALAACAALSPAAEPGEAAPALSGTFFSGQPFDLA
                     QMRGKVVLINFYSSYCSFCAYEIGNLENFYEEHHDQGFEVIVVGIDRPEDRPRVQRML
                     GIYGLPGTMADELAQSGFERKYPTPT"
     gene            464..1501
                     /locus_tag="VN496_14380"
     CDS             464..1501
                     /locus_tag="VN496_14380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF18192.1"
                     /translation="MAEATTGISADAQVARIFEWRRGFNAIHLIDLGNHLGLFRTLAQ
                     TPGLSGSELAGKLNLHAPYVEVWCKTAYGLEMLDTDEKGKYRLAPHFDSILASPGHPR
                     YLGGYVRLGTEVAADDFRRCRDAFKSGKAKPFQGRGEHFNQAIAESTQGLQIVTAKKI
                     LPALADLSQRLGAGGAILEIGCGTGNFLLQAAKAFPGARLVGVDIDDESLELARDKIQ
                     QAGLTGRISIHREKVDDAAGAFDAVVMIEVLHEIAPGIRPAVVGAAARTLKPGGWMVI
                     IDETYPTTLEEARRPEFRFPLQTGFEELIWGNVIPTREEQEKLLRDAGLKGEINRELI
                     GEGFTVLTVRR"
     gene            complement(1541..2608)
                     /locus_tag="VN496_14385"
     CDS             complement(1541..2608)
                     /locus_tag="VN496_14385"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXF18193.1"
                     /translation="MQKILKPAFVSMLFMLACIGSARAHTGGMTGFATITISDKLVRY
                     NLTLSDVPPGPLAEQMRLGKPGVTPDFKPLISAISEKIHFTSDGSSCRATDGRIVPPT
                     TDSVSLTGTVDFICSAEISKLTIRDDMSEIMGSSQHTLAAIVWAGGSQQFAFGSDARE
                     TTVAVSEAAKASRGAGSFFPLGIEHILTGYDHLLFLLALMLRGGGLWSLLKIITAFTI
                     AHSITLALAALDVVVLPGAFVESVIALSIAYVALENLLPRYAVSRRWAVSFLFGLVHG
                     FGFSSVLREIGLPKENLVLSLLNFNLGVEAGQLTVVLLAVPILMRLRSRKWEPSMVAT
                     VSGVILAVGLALFIDRAFLGG"
     gene            complement(2719..3327)
                     /locus_tag="VN496_14390"
     CDS             complement(2719..3327)
                     /locus_tag="VN496_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18194.1"
                     /translation="MNVAKANPGRRKLLAVWLLLAVLLITIVLVERHDRRVSETPKTG
                     RDERLLIPVDIKELGAIEIAHGGTLHRFERDATGAWFYHGIHTPAQPEHAHTADAVMS
                     ARIEKAFAGLGRARMERQFPLNLQADEFGVTRPEMFIMVYLPKQLQPLSRYAIGTVAP
                     DKVSRYVLPVGSSWVVTIADFQIDNLLSLIKAVSSGAAPAKK"
     gene            complement(3324..>3893)
                     /locus_tag="VN496_14395"
     CDS             complement(3324..>3893)
                     /locus_tag="VN496_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18195.1"
                     /translation="HEITRDLPLTFFPGARSLSPTPERVPGTAVRPVINSSKTSFGGT
                     NRERAEFNKEKDKTGPLTLMVAVNRRPEFVASTESVTRPNPAIPLAPQNQDAKSAASK
                     SSRVAIIGDSDFATNSFFHILGNGTLFLNTVNYLAAQENLIGLEPRTYDLPRVDLSNR
                     QMKATFFLSIILIPALMAVVGIAVWWRRR"
BASE COUNT          881 a         1076 c         1121 g          815 t
ORIGIN      
        1 agttggcgtc ggatacttgc gttcaaaacc gctttgcgca agttcgtccg ccattgtgcc
       61 cggcaatcca tagatgccga gcatgcgttg tacacgaggc cgatcctcag gcctgtcgat
      121 gccgacgaca ataacctcga acccttggtc gtgatgttcc tcataaaagt tttcaaggtt
      181 gccgatttcg tatgcgcaga aactgcaata actggaatag aagttaatca gcaccacttt
      241 gccgcgcatt tgcgccaaat cgaacggctg tcccgaaaaa aacgtgccac tcaatgccgg
      301 cgccgcttcg cccggttccg cagccgggga caatgcggcg catgctgcga gagcaacggc
      361 ggccaggcac ttcagtttca tcatgcagtg aataacgctg gtcaaataaa ctaattgcac
      421 cagtatactt tgactccatt tccactgata aaaagggcac gccatggccg aggcgacaac
      481 aggaataagc gcggacgcgc aagtggctcg catctttgag tggcgccgcg ggtttaatgc
      541 catccacctt atcgacctgg gtaatcatct gggattgttt cggacactgg cacagacgcc
      601 gggactgtcc ggcagtgaat tggcaggaaa gctaaacctg cacgcgccct acgtggaagt
      661 ctggtgcaag acggcttacg ggctggaaat gctcgataca gacgagaagg gaaaataccg
      721 tctcgcaccg catttcgatt caatccttgc gagtccgggc catccccgct atttgggcgg
      781 ttacgtgcgc ttgggtaccg aagtagccgc ggatgatttt cggcgttgcc gggacgcttt
      841 caaatccggc aaagcgaaac cattccaggg caggggagag catttcaatc aggctatcgc
      901 ggaatccacg cagggcctgc agatcgtcac ggccaaaaaa atattgcctg cgcttgctga
      961 tctgtcgcag cgccttggtg ccggcggcgc cattctcgaa attggctgcg gtaccggcaa
     1021 ttttctgttg caggctgcga aagcgtttcc aggagcgcgc ctggtcgggg ttgatatcga
     1081 cgacgaaagt cttgagctgg cgcgggacaa gatacagcag gctggcctca ccgggcgaat
     1141 ttcaatacac agagagaagg ttgacgacgc cgcaggcgcc ttcgatgcgg tcgtcatgat
     1201 cgaagtgctg catgaaattg cgcctggcat tcgccccgct gtagttggtg cggccgcgcg
     1261 gacgctgaag ccaggcggct ggatggtgat cattgatgaa acctatccga ctaccctgga
     1321 agaggcgcgg cgtcccgaat tccgctttcc actgcagacc ggctttgagg agttgatctg
     1381 gggcaacgta attcccactc gggaagaaca ggaaaagctg ctgcgcgatg cgggactgaa
     1441 aggtgaaatt aatcgtgaac tgatcggcga gggcttcacg gtgctgacgg tacggcggtg
     1501 atgcttcagc ggtgaatgtt cagcggtgaa tgtcaagcag ctaaccgccc agaaaggctc
     1561 ggtcaataaa cagtgccagt cccaccgcga gaatcacgcc ggagacggtt gctaccatgg
     1621 agggttccca ttttctggat ctgaggcgca tcaggatcgg aactgcaagc agcaccaccg
     1681 tcaattgtcc cgcttcaacg ccgagattga agttcagcag cgacaaaacc aggttttcct
     1741 tcggcaatcc gatttctcgc aataccgagg aaaacccaaa tccgtgcacc aacccaaaca
     1801 gaaagcttac ggcccaacgt cgcgacaccg catagcgcgg caacaggttc tccaaagcga
     1861 cgtatgcgat cgagagtgca atgacggatt caacaaacgc ccctggcaat acgaccacat
     1921 ccagtgccgc aagcgccagc gttatgctgt gggcaatggt gaacgcggta atgatcttca
     1981 gcagcgacca cagtccgccg ccgcgcaaca tgagtgcgag caggaacagc aagtgatcgt
     2041 agccggtcag gatgtgttct attccgaggg gaaagaagct gccggctcct cgggaggcct
     2101 tagctgcctc ggatactgcg acggtggtct cacgcgcgtc actgccaaag gcaaattgct
     2161 gactgcctcc tgcccatacg atagcggcaa gcgtgtgctg gctactgccc ataatttcgg
     2221 acatgtcgtc acgtatggtt agcttgctta tttcagccga acagatgaaa tccactgtgc
     2281 cagtcaggct gactgagtca gtggtcggcg gcactatgcg gccatctgtg gcgcgacagg
     2341 acgatccatc gctggtgaaa tgaattttct cgctgatggc gctgatcagc ggtttgaaat
     2401 caggtgtgac gcccggcttg ccaagccgca tctgttcggc aagcgggcca ggcggtacgt
     2461 ccgacaaggt aaggttgtaa cgcaccagct tgtcgctgat ggtgatagtg gcgaacccgg
     2521 tcatgcctcc ggtatgggca cgtgcactcc cgatgcaggc aagcataaac agcatgctga
     2581 cgaatgccgg ttttaatatt ttctgcacgg tagggtgttt tatgtttccg caatgggcaa
     2641 acgcagcggg atgccgccgc aaaaaatgct tccctccatt gcacccattc gcccgccggc
     2701 cattgctgct tcgcaaagct acttcttcgc gggcgcggcg ccgctactga ctgctttgat
     2761 gagagacagc agattatcga tctgaaaatc cgcaatggtc actacccagg aactgccgac
     2821 tggcaaaacg tagcggctta ctttgtctgg cgccaccgtc cctatcgcgt aacgggacaa
     2881 tggctgcaat tgcttcggca agtaaaccat gatgaacatt tccggccgcg tcacgccaaa
     2941 ttcgtctgcc tgaaggttga gcggaaattg ccgttccatg cgcgcgcgcc caagcccggc
     3001 aaacgctttt tcgatgcgtg cggacattac cgcatctgca gtgtgcgcat gttccggttg
     3061 tgctggagta tggatgccat ggtagaacca tgcgcctgtc gcatcccgtt cgaaccggtg
     3121 cagagtcccc ccatgagcta tttcgatggc gccaagttcc ttgatatcca ccggtatcag
     3181 caggcgctca tcgcgcccgg tcttcggagt ttccgacacg cggcgatcat gacgttccac
     3241 caaaacaatc gtaatcaaca gcaccgccag caacaaccat acggcaagaa gcttgcgacg
     3301 gcctgggttc gcctttgcga cgttcaacgc cgcctccacc acaccgcgat gccgacaact
     3361 gccatcagtg cggggatgag aatgatcgac aggaagaacg tggctttcat ctgccgattg
     3421 ctgagatcga ctcgtggcag gtcgtaagtg cgcggttcaa gaccgataag gttttcctgg
     3481 gcggcaaggt agttcaccgt gttcaggaac aatgtgccgt taccgagaat gtggaaaaaa
     3541 gagttggttg caaaatccga atcgccgatt atggccacac gggaggactt ggatgccgcg
     3601 gacttggcgt cctggttctg aggtgcaagc gggatcgcgg ggtttgggcg tgtcacgctt
     3661 tcggtagagg caacaaactc cgggcgccgg ttcacagcca ccatcagcgt aagtggccca
     3721 gtcttgtcct tttctttgtt gaattccgcg cgctcccgat tcgtgccgcc gaaactggtc
     3781 ttggaggaat tgattacggg acgcaccgct gtcccgggta cacgttctgg cgtcggtgac
     3841 aacgaccgcg cgcctggaaa aaacgtgagg ggcaaatcgc gggtaatctc atg
//
LOCUS       DATMZQ010000452         3230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4712142, whole genome shotgun sequence.
ACCESSION   DATMZQ010000452 DATMZQ010000000
VERSION     DATMZQ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4712142"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VN496_14400"
     CDS             complement(<1..213)
                     /locus_tag="VN496_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18196.1"
                     /translation="MKLRSLSTRMALVFTVMFIVVQAAVLVLVDTVSSRIARDRNTEE
                     LHIGARVLERLLDQNRQSLLQAAEVLS"
     gene            638..982
                     /locus_tag="VN496_14405"
     CDS             638..982
                     /locus_tag="VN496_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18197.1"
                     /translation="MVELLKSFGDQSVAKDRPKITAAQQALQERKVMTMNSLTHGAYY
                     NGVLDNMTTTARWHADKRRFILWETNMGEPKLNAAPHVADLGVGARFAPLSRQESDGA
                     SHISDFAFETTR"
     gene            complement(1221..1757)
                     /locus_tag="VN496_14410"
     CDS             complement(1221..1757)
                     /locus_tag="VN496_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18198.1"
                     /translation="MEIALITDSRLTSLGKKVRQIAGALVLGTLLLGFASGKSHAQAQ
                     PTALPQGQEQAPAGNFESVVRWINSRFTPAEIQKMSPDDFRIEPHACSCSDKPDPHFP
                     YLIVLFTTPKGDLVARPDGHENMTKFTPLAVRKGADYCMVDSEEQCYGSFANVCEFTD
                     FRYGSMLEQVFPTCKTAD"
     gene            2392..2979
                     /locus_tag="VN496_14415"
     CDS             2392..2979
                     /locus_tag="VN496_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXF18199.1"
                     /translation="MEKANPEHDQDSDPPRTAVSPDRPASGGRPTAVMRFVLRSADVR
                     EGETMSNTQVFHGMGCTGQNVSPALEWSGAPAATKSFAVTLYDPDAPTGSGWWHWVMY
                     NIPASVSKLPPGAGDPTREWLPGVTHGNTDFGKPGYGGPCPPVGDAPHHYIFTVHALD
                     VEQLDVPANATAALVGFNMHHHLLGRATLTGRYGR"
BASE COUNT          696 a          964 c          917 g          653 t
ORIGIN      
        1 cgagaggact tctgcggcct gcagcaaact ctgacgattt tggtccagca gacgttccag
       61 cacgcgagcg ccaatgtgca attcctcggt attgcgatca cgcgcaatcc tggagctgac
      121 ggtatcgacc agcaccagta cggcggcctg caccacgata aacattaccg tgaagaccag
      181 ggccatgcgt gtgcttagac tgcgtaactt catcccctac ctctgctatt tataggaccc
      241 tgctgggccc ggcggggaat tgtcagacct tctcacagct cgaatgcgga tgacgatatt
      301 ccgtcgactg catcggatat cggcattcga ttggcggcgc gattgcgttc cgccgcatag
      361 cgtggaaagc gctgcgatcc tccccaggat cccatctgca tatgagcaag gagggggcca
      421 gcttcgcgat cgccgctcta ccgcactaca gcaaacactt cagcggggtg tgatgcatca
      481 gcgtgctggt cgtttggggc tgccgttggg cggcagaacc gtttgtcagg cgatttcgcc
      541 cattcggcgg atatggctgt tcatgacgtc caaataggga ttgaatagca gcataccggt
      601 cgtgtccggg caagggaggc ttgcgaaagg attcaatatg gttgaactgt taaagtcgtt
      661 cggcgaccag tcggtcgcca aagataggcc caaaattact gcggcgcagc aggccttgca
      721 ggaacgcaaa gtaatgacga tgaattcgct gacgcatggt gcgtactaca acggtgtgct
      781 ggacaacatg acaaccacgg cgcgctggca cgcggacaag cgacgcttca tcctttggga
      841 aaccaatatg ggcgaaccta aattgaacgc ggcgccgcat gttgcggacc ttggcgttgg
      901 cgcgcgcttc gctccacttt cccggcaaga gtccgacggc gcatcacata ttagcgactt
      961 cgcgtttgaa accacgcgct gaacctgcga acaaaccaga aaagcaaacg cagattcccg
     1021 ccaccgcaag tcgacacggg catgaagtcg cgcccagaga tttcgcggtg ataccagatt
     1081 cgtgaacgct agcacgctag aattgcgtgc ttttattttc tagtctcgcc gcttttccga
     1141 aaacgcgtcc atcaaagtgc aacgtgcggg cagaacccgc cggcattcgt cccgctcacg
     1201 gcgcccgcac tatccgcgtc tcaatctgcc gtcttgcacg tcgggaatac ctgttcgagc
     1261 atcgagccat agcggaaatc ggtgaactcg cagacgttcg cgaatgaccc gtagcactgc
     1321 tcttccgaat ccaccatgca atagtccgct cccttgcgta ccgccaacgg ggtgaacttg
     1381 gtcatgttct cgtgaccatc aggcctcgca accagatcgc ccttgggggt ggtgaacagc
     1441 acgatgaggt aagggaaatg tggatcgggt ttatccgagc aactgcaggc gtgaggttca
     1501 atgcgaaaat cgtccggcga cattttctgg atttctgccg gcgtaaacct gctgttgatc
     1561 catcgcacga cgctttcgaa attgccggcc ggcgcctgct cctggccttg cggcaatgct
     1621 gtcggttgcg cttgagcgtg cgacttgcca gatgcaaaac ccagcagcag cgtcccgagc
     1681 acgagcgctc cggcaatttg acgtactttt tttcccagac tggtcaggcg ggaatccgtt
     1741 atcaatgcta tttccatgct tactcctatt ttctactaga ctccctgctg aatcaggctg
     1801 cccgatagca aacgcagtcg taccgatttt ccgattccgc gcaaatcctt ttgcagtatc
     1861 cggaccggga acacttgcgc cgttcgcgac agttccatac aaattgcatg cccgctggcc
     1921 aatcgtacgc cgttggcata tcaaaacgtg gactgataac aggggcttgc ggcatggata
     1981 accgtcccgc acgtagattc ttgcccgggt ttggactacg tcactggcgt gctgcgcaaa
     2041 caaattccaa ccagcacggt ccacacaggc cgaaagcctg ccgtccatcg cctatggcct
     2101 ggggccaagt tttgctgggg gctggatcgc acgcaccaaa tcgagtgggc ttttgctaac
     2161 acgtgagcaa atccaatcag cggtccgccg caacggaaga atctgcagtc ttgctgcaag
     2221 acttacaggg attgagcgtg tgcttctcgt agtggctgag tggaattgga gttcgcctcc
     2281 gcgtttcccc gcaggcttcg tggatcgggt gcaccggcag cgccccggca gtgaaaagca
     2341 gtgcttgcat gttctttgca cgatgcgctg aatcgccaaa ggagcccgat tatggaaaaa
     2401 gccaatcccg aacatgatca ggatagtgac cctccgcgca cggccgtgtc ccccgataga
     2461 cccgcctctg gcggcaggcc gaccgccgta atgcgtttcg tcctgcgcag cgcggatgtg
     2521 cgtgaaggag aaacaatgag caatacgcaa gtctttcacg gcatgggctg caccggacag
     2581 aatgtttccc cggcactcga gtggagcggc gcgccggccg ccacaaaaag ctttgcggtt
     2641 acgctctatg atccagatgc gccgaccggc agcggctggt ggcactgggt catgtataac
     2701 attccggcga gcgtgagcaa gcttccaccg ggtgccggag acccgacgcg cgagtggttg
     2761 cccggggtga cacacggcaa tactgatttc ggcaagccgg gatacggcgg accgtgtccg
     2821 cccgttggcg atgcgcccca ccactacatc tttaccgtgc acgcgctcga cgtggaacaa
     2881 ctcgacgtgc cagccaatgc cacagcggcc ctggtcggtt tcaacatgca tcatcacctg
     2941 cttggcagag cgaccctgac cggcagatac gggcgctaac catggcagca cgcaagagct
     3001 ctgcggccgg caaacgcagg ccgcgccgtc cggcaaagcg ctggtccgcg ggcgtaaccg
     3061 gctccagcaa tgcgctcgat ctggaaccgg gcgtattcaa gcacgatagc ccgaaagcaa
     3121 tcgcgcgagc gctcagcgga agccagtcac cgccgcaaag ctgccgtacc gctcggccat
     3181 gtccatgctg acgttctaca tcaaccgtgc tggaaccaat cttccgaaga
//
LOCUS       DATMZQ010000453         5018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4712151, whole genome shotgun sequence.
ACCESSION   DATMZQ010000453 DATMZQ010000000
VERSION     DATMZQ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4712151"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VN496_14420"
     CDS             <1..265
                     /locus_tag="VN496_14420"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18200.1"
                     /translation="PYPGAFTTVAGKNVRVLKTLLQPARHASFASPTLFVDGDACMAQ
                     CADGSVLRLVELELGGRRLAPDDITSALGPGPLPLLSKTMSLT"
     gene            262..1305
                     /locus_tag="VN496_14425"
     CDS             262..1305
                     /locus_tag="VN496_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HXF18201.1"
                     /translation="MKKILILGVNGFIGHHLSRRILDSTDWSVFGMDMQSERVATLME
                     HERFHFFEGDITINKEWIEYHIRKCDTVLPLVAIATPATYVKEPLRVFELDFEANLPI
                     VRHAVKYGKRVIFPSTSEVYGMSRDSEFDPDRSELVYGPINKPRWIYACAKQLMDRVI
                     AAYGQQEGLDYTLFRPFNWIGAGLDSIHTAKEGSSRVITQFFGHIVRGESIKLVDGGT
                     QKRAFTYIDDGIDALMKIIENKGGIASGRIYNVGNPKNNYSVRELAKMMIDLALLYPE
                     YRDAAKKVTTIETTSDAYYGNGYQDVQNRVPKIDNTRKDLGWEPKIDMDTALKNIFDA
                     YRSHVADARSLMD"
     gene            1380..2294
                     /locus_tag="VN496_14430"
     CDS             1380..2294
                     /locus_tag="VN496_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXF18202.1"
                     /translation="MKLALKIDVDTYRGTRHGVPHLVEILKERQAGATFLFSLGPDHT
                     GRAITRVFRNGFLGKVRRTSVVSHYGIKTLLYGTLLPGPDIGRECAQIMRDTRDTGFE
                     VGIHTWDHVLWQDNVARSDAQWTQRQMELACERFQEIFSEPARIHGAAGWQMNRHAYR
                     LTQRLGFSFCSDTRGSEPFVPVYQGEIIACPQLPTTLPTLDELIGVDGVTSRNVVDRL
                     LELTREPHPAGHVYTLHAELEGMKLAPIFENLIDGWQDQGHELVSLGSYLESLASNVL
                     PRHEIAVGPVPGRSGTLALQGREFLAGA"
     gene            2396..2863
                     /locus_tag="VN496_14435"
     CDS             2396..2863
                     /locus_tag="VN496_14435"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXF18203.1"
                     /translation="METSLVGLKVPDFESASTGTATFKLSAHLGSPLVLYFYPKDNTP
                     GCTTEGQQFRELHPEFTRLGCAIYGVSRDSVKSHEGFKSKMDFPFELLSDPDEAVCAL
                     FGVMKMKNMYGRQVRGIERSTFVIDSRGTLCKEWRGVKVPGHAQEVLEFVRSL"
     gene            2972..4402
                     /locus_tag="VN496_14440"
     CDS             2972..4402
                     /locus_tag="VN496_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXF18204.1"
                     /translation="MTQSRASAPTPVSTLRPRNKLFVLDTNVLMHDPTSLFRFEEHDI
                     YLPMVTLEELDANKKGMSEVARNARQVSRFLDEIVSKSVDDISVGIPLEVSGAKEATG
                     RLFLQTESINGELPARLATGKADNQIIGVVKHLQETLDKRHVILVSKDINMRIKARAL
                     GFAAEDYFNDKVLEDTDLLYTGVRELPADFWDQHGKEMESWQQEGRTYYRVHGPLCDS
                     LMVNEFVFLEGEKPFYAQVREQSGKTAILQTIRDYAHQKNNTWGIVARNREQNFALNL
                     LMNPDVDFVTLLGQAGTGKTLMTLAAGLMQTLEDKVYSEIIMTRVTVPVGEDIGFLPG
                     TEEEKMTPWMGALDDNLDVLNKTDDEAGEWGRAATRDLIRSRIKVKSLNFMRGRTFIN
                     KFLIIDEAQNLTPKQMKTLITRAGPGTKVVCLGNISQIDTPYLTEGSSGLTYVVDRFK
                     GWTHSGHVTLMRGERSRLADRAAEVL"
     gene            4506..>5018
                     /locus_tag="VN496_14445"
     CDS             4506..>5018
                     /locus_tag="VN496_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18205.1"
                     /translation="MKRVLRPILPHTAWQAIAALAGFFTLVGAMGAPADLWRLTVKSR
                     QGEPFAATATLQALPDEHISEACLSLGSESDAAGKKVPLLTRARLQFDAANGTVDIST
                     DEPVTVPALSLVLRVQCPGSPLYVRRFSASIPPAVNAQARIPAVERDMSKGFHLALLP
                     GDTVESIAAVL"
BASE COUNT         1123 a         1424 c         1432 g         1039 t
ORIGIN      
        1 accctatccg ggtgccttca ccaccgtggc aggaaagaac gtgcgcgttc tgaaaacact
       61 attgcaacct gcccggcacg ccagctttgc ttcacccacg ctattcgtcg acggagacgc
      121 ttgcatggcg caatgtgcgg acggcagtgt tttgcgcctc gtcgaactgg aactgggcgg
      181 acggcggctc gcgccagacg atattacttc cgccttaggc cccggccctc ttcctttgct
      241 ttccaaaaca atgagcctta cgtgaaaaaa atcctgatac tcggcgtgaa cggtttcatt
      301 ggccatcatc tgtcccggcg cattctcgat tccacggatt ggtccgtgtt cggcatggac
      361 atgcagagcg agcgcgtggc aacgctgatg gagcatgaac gctttcactt cttcgaaggc
      421 gacatcacca tcaacaagga atggatcgaa tatcacatcc gcaaatgcga caccgtgctg
      481 ccgttggtcg ccattgccac gccagcgaca tacgtgaagg aaccgctgcg ggtattcgag
      541 ctggatttcg aagcgaatct gcccatcgtc cgacatgcgg tgaagtatgg aaagcgcgtg
      601 atttttccct cgacatccga agtgtacgga atgagccgtg acagcgaatt cgatcccgac
      661 cgttccgaac tggtgtacgg cccgatcaac aaaccgcgct ggatctatgc ttgtgccaag
      721 caactcatgg atcgggtcat cgcagcctac ggtcagcagg aagggctcga ttacacgctg
      781 tttcgcccgt ttaactggat cggcgccgga ctggacagca ttcacaccgc caaggaaggc
      841 agttcacgcg tgatcacgca gttcttcggc catatcgtgc gcggtgaatc catcaagctc
      901 gtggacggcg gcacacaaaa acgcgcattc acttatatcg atgatggaat cgatgccttg
      961 atgaagataa tcgaaaacaa aggcggtatt gccagcggca ggatctataa cgttggcaat
     1021 ccgaaaaata attattcggt tcgtgagctg gccaaaatga tgatcgatct tgcgctgctg
     1081 tacccggagt acagggacgc ggcaaagaaa gtcacgacaa tcgagacgac ctccgatgcc
     1141 tactatggca acggctatca ggatgtgcag aaccgggttc cgaagatcga caacacgcgc
     1201 aaggacctgg gctgggagcc aaaaatcgac atggatacgg cgctcaagaa tatcttcgac
     1261 gcctaccgca gccacgtcgc tgatgcacgc agtctgatgg attaacgcgg gcttgccaga
     1321 acagtgctgc cacgacaaag caacagtttg ctcgccttta gtccgacaga tttatcttca
     1381 tgaagctcgc gctcaaaata gacgtcgata cttatcgcgg aacgcgccac ggcgtaccgc
     1441 atctggtgga gatactcaaa gagcgccagg cgggggcaac attcctgttt tcgctcggtc
     1501 cagaccatac tggcagagcg attacgcgcg tatttcgaaa tggttttctg ggcaaagtgc
     1561 gccgcacttc cgtggtaagc cattacggta tcaaaaccct gctctacggc acgctgctgc
     1621 cgggaccgga catcgggcgt gaatgcgcgc agatcatgcg cgatacgcgg gatacgggat
     1681 tcgaagtcgg catccacacc tgggatcacg tattgtggca ggacaatgtg gctcgctcag
     1741 atgcgcaatg gacgcagcgg cagatggaac tcgcctgcga acgctttcag gaaatcttca
     1801 gcgaaccggc gcgcatccat ggcgccgctg gctggcagat gaatcggcat gcataccggc
     1861 tcacgcaacg gctcggtttc agtttctgtt cggatacgcg tggcagcgaa ccgttcgtgc
     1921 cggtctatca gggcgaaatc attgcgtgtc cacagctgcc gaccacgctg ccaacgctgg
     1981 atgaattgat cggcgtggat ggtgtaacga gccgcaatgt tgtcgaccgg ctgctggaac
     2041 tgacccggga accgcatccc gcgggccacg tctacacgct acatgcagaa ctggaaggca
     2101 tgaaactcgc cccgatcttc gagaacttga tcgacggctg gcaagaccag gggcatgagc
     2161 tggtttcgct gggttcctat ctcgagtcgc tcgccagcaa tgtactgccc cggcatgaga
     2221 tcgccgttgg tccggtgccg gggcgcagcg gcacattggc gttgcaagga agagagtttc
     2281 ttgccggcgc ctgaatttct tagctggcct cggacaattg gcatcggtgg tccatgattt
     2341 ctttttgcgg cgctgttgca ttcccatagt gtttcggtaa ccatagcttc aggtaatgga
     2401 aacttcgctg gtcggcctca aggttcccga tttcgaatct gcttcgacgg gcactgcaac
     2461 gttcaagctc tcagcacatc tgggaagccc gctggttctc tacttctacc cgaaagacaa
     2521 caccccaggc tgtactaccg aaggccagca gttccgtgaa ctgcacccgg aattcacacg
     2581 gcttggctgc gccatctatg gcgtttcgcg cgacagcgtc aaatcccatg aagggttcaa
     2641 gtccaaaatg gactttcctt ttgaattgct gtccgacccg gatgaggctg tctgcgccct
     2701 gttcggagtc atgaaaatga agaacatgta cggccggcaa gtcaggggca tcgagcgcag
     2761 cactttcgtg attgatagcc gcggcacgtt atgcaaagaa tggcgcggtg tgaaagtgcc
     2821 cggccatgcg caggaggttc tcgaattcgt aagatccctt taaccaacag cacaggagcg
     2881 tgacaatgcc cagaaggagt aatgccttca ttcactcccg cattcaagtc attccgtctt
     2941 cgggtttttc gttcttaatc ggagcccagg catgacccaa tccagagcca gcgctcccac
     3001 gcccgtcagc acgcttcgtc cccgcaacaa gctgtttgtc ctggatacca acgttctgat
     3061 gcacgatccc acgagcctgt tccgcttcga ggaacacgac atctatctgc cgatggtgac
     3121 gctcgaggag ttggacgcca acaagaaagg catgtctgaa gtcgcccgca atgcgcgcca
     3181 ggtgagccgt tttctcgatg aaattgtttc caagtcggtg gatgatattt cggttggcat
     3241 cccgctggag gtctccggcg ccaaggaggc gacgggacgg ctgttcctgc aaaccgaatc
     3301 gatcaacggc gagttgcctg ctcgcctggc caccggcaag gccgataacc agatcatcgg
     3361 cgtcgtcaag cacttgcagg agacgctgga caagcgccat gtcatcctgg tcagcaaaga
     3421 catcaacatg cggatcaagg cgcgcgcctt gggctttgcc gcagaggatt atttcaatga
     3481 caaggtgctg gaagacaccg acttgctgta caccggcgta cgcgaactgc cggccgattt
     3541 ttgggatcag catggcaagg aaatggaatc ctggcagcaa gagggccgta cctattatcg
     3601 cgtgcacgga ccgctgtgcg acagcctgat ggtgaacgag tttgtgttcc tcgaaggcga
     3661 aaaaccgttc tacgcccagg tgcgggagca aagcgggaag accgcgatcc tgcaaaccat
     3721 tcgagactat gcgcaccaga aaaataacac ctggggaatc gttgcccgca atcgcgaaca
     3781 aaattttgcg cttaacctgc ttatgaatcc ggatgtggat ttcgtgaccc tgctcggtca
     3841 ggccggaacc ggcaagacac tcatgacgct ggccgctggg ttgatgcaga cactggaaga
     3901 caaggtctat tccgaaatca tcatgacgcg cgtcacagtg ccggtcggcg aagacatcgg
     3961 cttcctgccc ggcacggagg aagaaaaaat gacgccgtgg atgggagcgc tggatgacaa
     4021 cctcgacgtg ctgaacaaga ccgacgatga ggcgggcgaa tggggccgcg ctgccacgcg
     4081 cgacctgatc cggtcgcgca tcaaggtcaa atcactcaat ttcatgcgcg gccggacatt
     4141 catcaacaag ttcctcatca tcgatgaggc gcagaatctg acgcccaagc agatgaagac
     4201 gctgattacg cgcgccggtc ccggaaccaa agtggtctgc ctcggcaata tttcgcaaat
     4261 cgacacgcct tatttgaccg agggtagttc aggcctgact tacgtggtag accgcttcaa
     4321 aggctggaca cacagcggcc acgttacgct gatgcggggc gaacgctcgc gcctggcgga
     4381 tcgcgcggcc gaagttctgt agcccatatt ggccgcgctt cgagggcgtt gcgccagcgc
     4441 ttcgatcggg ctaaactctc ggataccaca tatggtgctt atgcactgaa accctacaac
     4501 atttagtgaa gcgcgtgctt agaccgatcc tcccgcacac ggcgtggcag gcgattgcgg
     4561 ccttggctgg ctttttcacc ctcgtcggcg ccatgggagc gccggcggac ttgtggcgcc
     4621 tgacggtcaa gtcacgccag ggcgaaccgt ttgcagcgac ggcgacactg caggcgctgc
     4681 cggatgagca tatttcagag gcttgcttgt cgctgggttc cgagagcgac gcagcgggaa
     4741 agaaagtccc gctactcacc agggccaggc tccagttcga cgcggccaat ggcacggtcg
     4801 atatcagtac tgatgaaccc gtcacggtgc ccgcattatc cctggtgctg cgagttcaat
     4861 gtccaggctc tccgctttac gttcggcgtt tttccgcatc gataccgcct gccgtcaacg
     4921 cacaggcgcg tatccccgcc gttgaacgcg acatgagtaa agggtttcat ctggccctgt
     4981 tgccgggcga taccgtggaa agtattgctg cggtgctg
//
LOCUS       DATMZQ010000454         7406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_915045, whole genome shotgun sequence.
ACCESSION   DATMZQ010000454 DATMZQ010000000
VERSION     DATMZQ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915045"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..379
                     /gene="rpmH"
                     /locus_tag="VN496_14450"
     CDS             245..379
                     /gene="rpmH"
                     /locus_tag="VN496_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXF18206.1"
                     /translation="MKRTYQPSKTRRARTHGFLERMKTRGGRAVINARRSKGRARLAV
                     "
     gene            720..929
                     /gene="yidD"
                     /locus_tag="VN496_14455"
     CDS             720..929
                     /gene="yidD"
                     /locus_tag="VN496_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXF18207.1"
                     /translation="MRALLLTLIRLYQLTISPLTGNSCRFYPSCSEYAKFAIEKHGAI
                     SGSWLATKRILRCHPWHPGGADPVP"
     gene            945..2597
                     /gene="yidC"
                     /locus_tag="VN496_14460"
     CDS             945..2597
                     /gene="yidC"
                     /locus_tag="VN496_14460"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXF18208.1"
                     /translation="MDSLRLFAAMVLAVSVFLLFDAWNKERSPQSPGSEQSAQRSDSS
                     MPSPTAPPSVVAPTGPGNPSANNSSDANRAPITVRTDVLIAEIDPVGGTLRRLEFTQH
                     RDKLDKSRNFVLFQKDAVHTYLAQSGLIGQDLPNHTTPYSSPAMNYELHEGSNELTVS
                     LTALGTKAVGVTKTLTFHRSSYLIDEKYQIANRGAAEIKPFAYFQLVRDGTPAAGDSK
                     MVPTYTGAAVYTDESKFHKIAFSDIDKGKNDYPKHSNDGWIAMVQHYFLAALLPSGSV
                     SREFYTKSLGGGLYTVGVILPAGAIAPGASESIATRLYAGPQEQDKLAELAPGLNLTV
                     DYGWLTVIATPLFWVLSAIQKWTGNWGVSIILLTVIIKLIFFPLSAASYRSMAKMRVM
                     APKMQKLKEQYGDDRQRLHQAMIEMYKTEKINPLGGCLPIVVQIPVFIALYWVLLASV
                     ELRNAPFIWWIRDLSAQDPYYILPVLMGATMIIQTYLNPTPPDPIQAKVMKIMPVAFS
                     VFFFFFPAGLVLYWLVNNILSIAQQWQITRMIERSKSAHVAR"
     gene            2584..3924
                     /gene="mnmE"
                     /locus_tag="VN496_14465"
     CDS             2584..3924
                     /gene="mnmE"
                     /locus_tag="VN496_14465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXF18209.1"
                     /translation="MWHADTIAAIATPPGRAGIGVVRVSGPNTRDIAAGVLNRELRTR
                     VATLCDFFAEDFAPLDRGIAIFYAAPHSYTGEDVLELQGHGGLAVMQLILRRCFALGA
                     RPARPGEFTQRAYLNDKLDLAQAESVADLIDASSEAAARGAMRSLAGEFSGHVGRLRD
                     AIIELRSLIEATLDFPEEELDVLDRSGAREKLRRLQHEVATLLAQAEQGRILREGAKV
                     VLIGRPNVGKSSLLNRLAEEEVAIVTEIPGTTRDPLRHELAIEGVPVHVVDTAGLREA
                     TDTVEKIGISRAWLEIERADLALLIVDAGAGVGQADLDILAELPKKIHIIIILNKIDI
                     LGIQPSQVIGPDRTEIWLSAKSGEGVNLLRGAILRCIGWQSTAEGTFIARERHLQALR
                     DAAMCLNRAALQAGSLELLAEELRLAQQVFTDLTGKFTADDLLGEIFSRFCIGK"
     gene            3928..4692
                     /locus_tag="VN496_14470"
     CDS             3928..4692
                     /locus_tag="VN496_14470"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HXF18210.1"
                     /translation="MIELAALCTFAFLAGLVDAIAGGGGLIQVPALLAVYPTAPPSLL
                     LGTNKFSSITGTAIATLRYSFSVSIHWKTVLPAAAVAGLGAVAGAQTVRLIDPVLLRP
                     VLLTLLSGMVVHTLMSPTLGTEAVKDARIHRESRTFYLASAIFGFYDGFFGPGAGSLM
                     MFSLVRWFGYDFVGAAATTKVLNLVTNFGALALFVWTGNVIYAIALPMAACNVVGGFL
                     GAHFAIRRGSSFIRTIFLAVSVVLIGKVLADFYRSL"
     gene            4762..6687
                     /gene="mnmG"
                     /locus_tag="VN496_14475"
     CDS             4762..6687
                     /gene="mnmG"
                     /locus_tag="VN496_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXF18211.1"
                     /translation="MQNSNAFDVIVVGGGHAGTEAALASARAGCRTLLLTHNIETIGQ
                     MSCNPSIGGIGKGHLVKEIDALGGSMACATDHAGIQFRILNSSRGPAVRATRAQADRV
                     LYRQAVRKRIESQAHLVVFQQAVDDLIIDRNRVAGVMTELGIAFFARAVILTTGTFLS
                     GLIHVGLKNYAAGRAGDPPAIRLARRLRELALPVGRLKTGTPPRIDGRTIDYSVTTEQ
                     PGDNPVPVFSFLGLAAQHPTQLPCWITHTNEKTHEIIKEGLDRSPMFTGAIEGVGPRY
                     CPSIEDKITRFADKASHQIFLEPEGLTTHEVYPNGISTSLPFDVQIELVHSIRGLENA
                     RLTRPGYAIEYDYFDPRGLRSSLETKAIDGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     LSVQDKEPWCPRRDEAYLGVLVDDLITRGVTEPYRMFTSRAEYRLSLREDNADTRLTE
                     IGRKMGLVDDRRWHAFQSKREAIGVEHERLKSTRLNARLLSDEEMEQRFGQKLERDYT
                     FEELLRRPGVQYRHLQSLPGAVSPLLDEKVMEQVEIEIKYKGYIERQRDEVSGQKQFE
                     MTRLPEDLDYRSVRGLSIEVQQKLNKQRPETLGQAARISGITPAAISLLLVHLKRGLS
                     DSRRNKPALSGQAGTSL"
     gene            6726..7325
                     /gene="rsmG"
                     /locus_tag="VN496_14480"
     CDS             6726..7325
                     /gene="rsmG"
                     /locus_tag="VN496_14480"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXF18212.1"
                     /translation="MSLVLPGASIDRLTKYLQLLVKWNRVYNLTALRDEASLVTHHVL
                     DSLAVVEHLPGGNVVDVGSGAGLPGVPIAIACPGRAVTLIDSNNKKSAFLKQVIAELD
                     LSHVNVVTNRVEAYQPPELFSTVISRAFANLAAFVTLAGHLRAADGVMIAMKGLDPGE
                     EVARVPPSWKIISTLPLEVPQLGASRHLVFLRPAAIAPN"
BASE COUNT         1636 a         1948 c         2134 g         1688 t
ORIGIN      
        1 tatactgttg cgctggaagg gctttttcga aatggcgaag gcaatgattc cagaagctgt
       61 ccatcggcgc aaagatcggg attgtgatgc agggtgcggc cgttgggcca cttcgcacgt
      121 gctggcgaat tttaccagca agcagttgag ttatccacag accggcaata gttgacaagc
      181 gtgccataaa acccgtgtaa tacgcggctt tttctcgccc gatattcgcc ggagcacaaa
      241 atttatgaag cgcacctacc aaccctccaa gacacgtcgc gcgcgaacac acggctttct
      301 agagcgcatg aagacacgcg gcggaagggc tgttatcaat gctcgcaggt cgaaagggcg
      361 ggcacgtctg gcggtctgaa gcgggcgcgt cttcggctgc ctcgcaagtc ggcgttgtgc
      421 ggcaaaggcg agtttaccgg caagttcagc gcaagacggg aggggaaatt tttcgttgtc
      481 ttgagcaggc gtacctcttc gggggaacag gcccggctcg ggatagtcgt cggcagacag
      541 actgcgcctc gttcggtagc gcgttcgctg atcaagcgta ctgttcgaga agtatttcgc
      601 aaaatacgca atcagctggg ttcagttgat ttcgttgtac gagtccacaa gcccgcgggg
      661 ccgcctgatc tagccgctgc tcgccatgaa ctcgaaaggc ttctgcgaga aacttgctga
      721 tgcgagcact gctcttaaca ctgattcgct tgtaccaact tacgatcagt ccattgaccg
      781 gcaatagttg ccgcttctat ccaagttgtt ccgaatatgc caaattcgcg atcgaaaagc
      841 atggcgcaat cagcggaagc tggttggcga ccaaacgcat cctccggtgt catccgtggc
      901 acccgggcgg agccgatccc gtgccttgac aagaaattat cctgatggac agcttgcgtc
      961 tatttgccgc gatggtgctc gcggtttcgg tcttcttatt atttgatgcg tggaataagg
     1021 agcgaagccc ccaatcaccg gggtccgagc agtccgcaca gcggtcggac tcgtcaatgc
     1081 cttcccctac tgcgccgccg tccgttgttg cgccgaccgg gcccgggaat ccctcagcca
     1141 acaactcatc cgatgccaac cgcgccccga ttacagtacg aaccgatgtt ctgatcgcgg
     1201 agattgaccc cgtgggcggg acgcttcgca gactcgagtt tactcagcac agggataagc
     1261 tggacaaatc gcgcaatttc gtcctgttcc agaaagacgc ggtacacacc tacctggcac
     1321 agagcgggct catcgggcaa gatctgccga accacacaac tccttacagt tctcccgcaa
     1381 tgaactacga gttgcacgag gggagcaatg aactgaccgt cagtttgacg gccctcggca
     1441 ctaaagcagt gggcgtcaca aagacgctga cgtttcatcg aagcagctat cttatcgacg
     1501 aaaaatatca gatcgccaac cgcggcgctg ccgaaatcaa gccgttcgcg tattttcaac
     1561 tggtgcgtga cggcaccccg gcggccgggg attccaaaat ggttccgacc tatacgggtg
     1621 cggctgttta tacagatgaa tcgaaatttc ataagatcgc attttcggac atcgacaaag
     1681 gcaagaacga ctatcccaag cattcaaacg atggttggat cgcaatggtc cagcattact
     1741 tcctggccgc tttgttgccg tctggaagtg tgtcgagaga gttctacacc aagtcgctag
     1801 gcggggggct ctataccgta ggcgttatcc tgccagcggg agctattgcg ccgggcgcga
     1861 gcgaatccat agcgacgcgt ctttacgctg gtccacaaga gcaggacaaa cttgccgaac
     1921 ttgcgcccgg cctgaatctt acggtggact acggttggct gaccgtcatt gccactccat
     1981 tattctgggt gctctccgcc attcaaaaat ggaccggcaa ttggggagtc tcgatcatat
     2041 tgctgaccgt gattatcaag ctaatatttt tcccgctatc ggcagcgagc tatcggtcca
     2101 tggccaagat gagggtgatg gcaccgaaaa tgcaaaaact gaaggaacaa tacggggacg
     2161 accgccagcg tttgcatcag gcaatgatag agatgtacaa gacggaaaaa ataaacccgc
     2221 ttggcggctg cctgcccatt gtggttcaaa ttccggtgtt catcgcgctt tactgggtgc
     2281 tgctggcaag cgtggaatta agaaacgcgc cgtttatctg gtggataagg gatctttcgg
     2341 cccaggaccc ctactacatt ttgccggtat tgatgggcgc aaccatgatt attcaaacgt
     2401 acttgaatcc cacgcctccg gacccgatcc aggcaaaggt catgaagatc atgccggttg
     2461 ctttcagcgt gttctttttc ttcttccccg ccggactggt gctttactgg ttggtgaata
     2521 acattctgtc cattgcacag cagtggcaga ttacacgcat gatagagcgg tcgaaatccg
     2581 ctcatgtggc acgctgacac gattgcagcg atagccacgc ctcccggtcg tgccggcatt
     2641 ggcgtggtac gtgtctccgg cccgaacacc cgagatattg ccgcaggtgt tctgaatcgt
     2701 gagctgcgta cacgagtcgc cacgttatgc gatttttttg cggaagattt cgcacccctt
     2761 gatcgaggta tcgccatttt ttacgctgcg ccccattcct atacgggcga agacgtgctc
     2821 gagttacagg gccacggcgg ccttgccgtt atgcagctta tccttagaag atgttttgcc
     2881 cttggcgcgc gtcccgcgag gccgggggag ttcacgcagc gggcatatct gaacgacaag
     2941 ctggatctgg ctcaggcgga gagcgttgcc gatttaattg acgccagttc ggaagccgca
     3001 gcacggggtg caatgcggtc gctcgcaggg gagttctctg gtcacgttgg tcgcttacgc
     3061 gacgcaatca ttgagctgcg tagcttgatt gaagcaaccc tggattttcc tgaagaggaa
     3121 cttgacgttc tggaccgcag tggcgcacgc gagaaactgc gtcgactgca gcacgaggtg
     3181 gcgactttgc tggctcaggc ggagcaaggc aggatcttgc gcgaaggtgc caaagtcgta
     3241 ttgatcgggc ggcccaatgt cggcaagtcc agcttgctta atcgcctggc cgaggaggaa
     3301 gtcgcgatcg tcaccgaaat tcccggaacg acacgggatc ctttacggca tgagctggca
     3361 attgaaggag tgccggtgca tgtcgtagat acggctgggc ttcgcgaagc aacggatacc
     3421 gtcgagaaga ttggcattag cagggcctgg cttgaaatcg aacgtgcaga cttggcgctg
     3481 ttgattgtgg acgctggtgc cggcgtgggc caggccgatc tcgatattct cgcggagctt
     3541 ccaaaaaaaa tacatataat catcatctta aacaagatag atatattggg catccagcca
     3601 agccaggtta ttggaccgga ccgcacggaa atatggttat cagcaaaatc cggtgaaggg
     3661 gtcaatctac tgcggggggc tattttgcga tgcatcggct ggcaatctac tgcggagggc
     3721 acgttcattg cccgtgaacg tcatcttcaa gcactgcgtg acgcggcaat gtgcttgaat
     3781 cgcgctgcgc tgcaagcggg ctcgctcgaa ctgctggctg aggagttgcg tttggcccaa
     3841 caggtattca cagatctgac tggcaagttc acggcagacg atttgctggg ggaaatcttt
     3901 tcgcggttct gtattggcaa gtaaagcgtg atcgaactcg ccgcgctttg cacgttcgca
     3961 ttcctggccg ggcttgtgga tgctatcgcg gggggaggag gactcataca ggttcctgcc
     4021 ttgctcgcgg tgtaccctac tgcgccgcct tccctgcttc ttgggaccaa caagttttcg
     4081 tcaataacgg gaactgcgat agcgacgctg cgctatagct tttccgtttc catccactgg
     4141 aagacggtac ttcctgcagc tgctgttgcc ggtcttggcg cagtagcagg cgcacaaacc
     4201 gtgaggctga ttgatccggt tctattgcga ccggttttgt tgaccttact gagcggcatg
     4261 gtggtccata cgctgatgag cccaacactg ggtactgaag cagttaagga cgcgcgtata
     4321 caccgcgaaa gcaggacttt ttacctcgcc agtgcgatat ttgggttcta cgatggattt
     4381 tttggtccgg gtgctggcag cctaatgatg ttttcattgg tccggtggtt cggttatgac
     4441 tttgtcgggg cggcagcgac aaccaaagtc ctgaatctcg tcacgaactt cggggcgcta
     4501 gccttgttcg tttggaccgg gaatgttatc tatgcgattg cattgccaat ggctgcctgc
     4561 aacgttgttg gtggttttct gggcgcgcat tttgcgatac gccggggcag cagcttcatt
     4621 cgcaccatat tccttgccgt atccgtggtt ctgatcggca aggtgctcgc ggatttctat
     4681 agatctttgt agcttatgtt tcacgtggaa cgtcatcgga gagttccttt ccggtatcat
     4741 tcgccacccc gaaagtctgt tatgcaaaac tccaacgcat ttgatgtgat cgtggtaggt
     4801 ggtggacatg ctggcaccga ggctgccttg gcgtctgccc gtgccggctg ccgaacatta
     4861 ctgctaaccc acaacatcga gacgattggc caaatgtctt gcaatccttc cattggtggc
     4921 attggcaaag ggcatttagt gaaggaaata gatgccttgg ggggcagcat ggcctgcgcg
     4981 acggaccatg ccgggataca gtttcgcatc ctgaattcga gtaggggccc cgccgtccgc
     5041 gcgacgcgcg cacaggcaga tcgggtgctc tatcggcagg cggttcgcaa gcgcatagaa
     5101 agtcaggctc atctggtcgt ttttcaacaa gcggttgacg acctcataat cgacaggaat
     5161 cgcgttgccg gtgttatgac agagttgggt atcgcgtttt tcgcccgcgc ggtgattctg
     5221 acgacaggca ccttcctttc gggtctgatt catgtcggcc tcaaaaatta tgcggcgggt
     5281 cgagctgggg atcctcccgc aattcgcctg gcgcgtcggc tgcgcgaatt ggctcttccg
     5341 gttggcagat tgaagacggg cacgccgccg cgcatcgatg ggcggaccat tgactactca
     5401 gtgacaaccg aacaaccggg tgacaatccg gtaccggtgt tttcgtttct tggtctggcc
     5461 gcgcagcatc caacgcaact gccgtgttgg atcactcaca ccaacgaaaa aacacacgag
     5521 attatcaagg aaggtcttga ccgatcccca atgttcactg gcgcgataga gggagtaggg
     5581 ccgcgctact gtccgtcaat tgaagacaag attacccgct tcgcagataa agcgtcgcat
     5641 caaattttcc tcgaaccaga gggacttacg acgcatgaag tctatcctaa cggcatttcc
     5701 accagcctgc cgttcgacgt tcaaatagag ttggttcatt cgatcagggg tctcgagaac
     5761 gcgcgcctga cccggccggg ctacgcaatc gagtatgact atttcgaccc ccggggtttg
     5821 agatcgtcgc ttgaaaccaa agcaatagac ggtctgtttt tcgccggaca gatcaatggc
     5881 accaccggct atgaagaagc ggccgcgcaa ggcctcctgg cgggactgaa tgcggcactg
     5941 tcggtacagg acaaggaacc ttggtgtccg cgtcgcgatg aggcctacct tggcgtgctg
     6001 gtcgacgatt tgataacgcg cggcgttacg gagccttacc gtatgtttac gagccgagcc
     6061 gagtatcggc taagcctgcg cgaagacaat gcggatacgc gactaactga aattggaagg
     6121 aaaatgggtc tggtcgacga cagacgctgg catgcgttcc aatccaaacg cgaggctatt
     6181 ggagtggagc atgaacggct caagtccacg cggctaaatg caagactgct gtcggatgag
     6241 gaaatggaac aacgctttgg acagaaactg gagcgagact atacctttga ggaattattg
     6301 cggcggcctg gtgtgcagta tcgacatctg caatcactgc cgggcgctgt ttcaccgctg
     6361 ctggatgaga aagttatgga gcaggtcgag attgagatca agtacaaggg atatattgaa
     6421 cgccaacgcg atgaggtaag cgggcagaaa cagtttgaga tgacccgtct cccggaggat
     6481 ctcgactacc gttccgttcg gggcttgtcc atcgaggtgc aacaaaaact taataaacag
     6541 cgcccggaaa cgctggggca agcggccagg atctcgggaa taacgcctgc cgccatatcg
     6601 ttgcttcttg tgcatctgaa acgcggcctc agcgattcac gccgcaacaa gcctgcactt
     6661 tccggccaag ctggaactag cctttgaatc aaatcgtttc tatgttacgg caaggcattg
     6721 ccgatatgag tctggtattg cccggcgcgt ccatagaccg actgacaaaa tatctccagt
     6781 tgttggtcaa atggaatcgg gtgtacaacc tcacagcgct gcgggacgag gccagcctgg
     6841 tcactcacca tgtgctggac agccttgccg ttgttgaaca tttgccgggt ggaaatgtag
     6901 tcgatgtcgg tagcggcgcg ggtcttccgg gtgtgccgat tgcgatcgca tgtcccggcc
     6961 gagcggtcac gttgatcgat tcaaacaaca agaagagcgc gttcctaaag caagtcattg
     7021 cagaactaga cctgtcacac gtcaacgtag tgacaaacag agtggaggcg tatcaacccc
     7081 ctgaattatt cagtaccgtc atctcgcgtg cattcgcgaa cttggcggct ttcgtgacat
     7141 tggccgggca tctccgcgcg gcagacggtg tgatgatcgc aatgaaggga cttgatccgg
     7201 gagaagaggt ggcacgggta ccgccttcgt ggaaaattat ttcgacgttg cccctggaag
     7261 tacctcagtt gggagcgtcc cgtcacctcg tttttctgcg accagccgct atagcgccga
     7321 attgactcaa atggcacgtg tgttagcggt cactaaccag aaaggcggag tcggaaagac
     7381 tacgacaacg atcaacttgg ccgcca
//
LOCUS       DATMZQ010000455         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1578465, whole genome shotgun sequence.
ACCESSION   DATMZQ010000455 DATMZQ010000000
VERSION     DATMZQ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578465"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1112
                     /locus_tag="VN496_14485"
     CDS             114..1112
                     /locus_tag="VN496_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXF18213.1"
                     /translation="MHAKMRAMQLDAVSRTLVLHQIDRPIAGPGQVSIKVAACGVCRT
                     DLHVVDGDLPDTKSDVIPGHEIVGTIEAIGPGVDLLTLGERVGVPWLGWTCGECNYCL
                     SGRENLCDRARFTGYTLNGGYADHAVADARFCLKLPANYSDEEAAPLLCAGLIGYRAL
                     RAAGDARRIGIYGFGAAAHIITQVARHEGRELYAFTRPGDVTAQNFALQLGAAWAGSS
                     TEPAPNLLDAAIIFAPVGSLIPVALTAIVKSGIVVCAGIHMSDIPSFPYALLWGERRI
                     VSVANLTRADGEAFLALAPSVPVRSVTVQYPLAHANSALKSLRAGTLSGAAVLTME"
     gene            1203..1859
                     /locus_tag="VN496_14490"
     CDS             1203..1859
                     /locus_tag="VN496_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXF18214.1"
                     /translation="MLFESRADAARQLADRLSRYRGRNPLVLAIPRGAVGMGRSLADE
                     LEGELDVVLVRKLRSPFSAEFAIGAIDESGWTYITTEDAARSGADAAYLEREKAFQLG
                     TLRKRRAQYTPARQPIDPKGRVVICVDDGLATGATMIAALHAVGAKQPQRLVCAVPVA
                     PPETLERIRPLADEVVCVAVPEEFYAVGQFYRHFPQVEDEEVIDLLAQSQQKYAAGRP
                     "
     gene            complement(1941..2330)
                     /locus_tag="VN496_14495"
     CDS             complement(1941..2330)
                     /locus_tag="VN496_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HXF18215.1"
                     /translation="MLTREQLTQLRTMIDERHMALEAELHSDAERSREETYGELAGPV
                     ADAGDSASASLISDVGNAELRRDLQELRALEAARQRIDDGTYGSCVDCGQDIGFKRLL
                     AQPAALRCLSHQQAYEKTHVQAPKPTL"
BASE COUNT          476 a          776 c          815 g          529 t
ORIGIN      
        1 catttgagca ttggatcagg ttgggatgga tgactcgccg ccgcaccagg ctgccataac
       61 ttttcacttc tccgcaacgc cggcgcattc cagtcggacg cgcatgtaat gcgatgcacg
      121 caaagatgcg ggcgatgcaa ctggatgctg tcagccgcac attggtcctg caccagatcg
      181 accgaccgat tgcgggccca gggcaagtat cgatcaaagt agccgcctgc ggcgtatgca
      241 gaaccgattt gcatgttgtc gatggcgatt tgccggatac caagtctgac gtcattcctg
      301 gacatgaaat agtcggcacc atcgaagcga ttggaccagg cgttgacttg ctgacacttg
      361 gcgagcgggt tggcgttccg tggctgggat ggacctgcgg cgagtgcaac tattgcctga
      421 gtggccgtga aaacctgtgc gatcgcgccc gcttcaccgg ctacaccctg aatggtggct
      481 acgcggacca tgccgttgcc gatgcgcgct tttgtttgaa actgcccgca aattattccg
      541 acgaagaagc ggcaccgttg ctgtgtgccg gactgatcgg ctaccgggcg ctgcgcgccg
      601 ccggagatgc ccggcgcatc ggcatttatg ggttcggcgc agcggcccac atcattacgc
      661 aggtcgcacg acatgagggc cgcgaactct atgcgtttac gcgcccggga gacgtaactg
      721 cgcaaaactt tgccctgcag ctcggcgcgg cgtgggccgg atcgagcact gagccagcac
      781 ccaatctgct cgatgcggcg attatttttg ctccggtcgg cagcctgata ccggtggcgc
      841 tgaccgcaat tgtgaagagc ggcatcgtgg tgtgcgcggg aattcatatg agcgatatcc
      901 cgtcgtttcc gtatgcgctg ctgtggggcg agcggcgcat cgtctccgtc gcgaatctga
      961 cccgcgcaga tggcgaggct tttctagccc tggcgccgtc cgtcccggta cgcagcgtca
     1021 cggtccaata tccattggcc catgcaaaca gcgcgctcaa gtccctgcgc gctggcacgc
     1081 tgagcggggc agcggttctt acgatggaat gacaatgccg acggtctgcg cgtattggcg
     1141 cgggtttgat cttgcgcaaa gccgcagccg ttgccttggc agatcatgct tggcctatga
     1201 cgatcctgtt tgagtcccgc gcagatgccg cgcgccaatt ggccgaccgg ctttcccgct
     1261 atcggggccg aaatccactg gtgcttgcga tcccgcgcgg cgcggttgga atggggcgct
     1321 cgctcgcgga tgaactggaa ggcgaactgg atgtggtgct ggtgcgcaaa ctgcgctcgc
     1381 ccttcagcgc ggaattcgcc attggtgcaa tagacgagtc gggttggacc tacattacaa
     1441 ccgaagacgc tgcgcgttcc ggcgccgatg cggcatacct cgagcgcgag aaggcctttc
     1501 agctcgggac tttgcgtaag cggcgtgccc aatatacgcc tgctcgccag ccgatcgacc
     1561 cgaaaggtcg cgtcgtaatt tgcgtcgatg atgggctcgc cactggtgcg accatgatcg
     1621 cggctttgca tgcggttggc gcgaagcagc cgcagcgcct cgtgtgcgcg gtgccggtcg
     1681 ccccaccgga aaccctggag cgcatccggc cgctggccga cgaagtggtg tgcgtcgctg
     1741 tgcccgaaga gttctatgca gtaggccagt tctaccggca ttttccccaa gtcgaggacg
     1801 aggaagtgat cgatctgctg gcccagtcgc aacagaaata cgcagcaggc cggccatgag
     1861 cagcgccaat cgggaagcgc caaccacgct cgcggccgac aatgcgacgc tcgaaaatcg
     1921 ccgagcgggt gcttggtttc ctagagtgtc ggcttggggg cttggacgtg ggtcttctcg
     1981 tacgcctgct gatgtgaaag gcaacgcaat gccgccggtt gcgcaagcag cctcttgaat
     2041 ccaatgtcct gaccacagtc cacgcaggac ccgtaagtac cgtcgtcgat ccgctgccgt
     2101 gccgcctcca atgcacgcaa ctcctgcaag tcgcgcctca attcagcatt gccaacatcc
     2161 gagatgagac ttgcgctggc gctatcgccg gcgtcggcga ccgggccagc aagctcgcca
     2221 tacgtctctt ctcgcgaacg ttcagcgtcg ctgtgcagct ctgcttccag tgccatgtgg
     2281 cgttcatcga tcattgtccg caactgcgtc aactgctctc gtgtgagcac gtcttgctcc
     2341 tttcgcgttt caaggggggg ccgccgggtc gggcgcttga tccaggtcaa actcttgcgg
     2401 cgcgtttgag cctaaaaagc aggcgtgggg aagagtaata cgttgcatgg gccaggagca
     2461 atgcctcttg ttgcccaatc ggaaaaagga cctgtcggat cggggaggcg actgtacgcc
     2521 ttgcgaacac tgatgattga ccaaaccctc gaggaacagc gcaggctggt ccaggcgctg
     2581 ctgtctgctg actgct
//
LOCUS       DATMZQ010000456         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1579184, whole genome shotgun sequence.
ACCESSION   DATMZQ010000456 DATMZQ010000000
VERSION     DATMZQ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579184"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="dnaX"
                     /locus_tag="VN496_14500"
     CDS             complement(<1..1124)
                     /gene="dnaX"
                     /locus_tag="VN496_14500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXF18216.1"
                     /translation="MTLALARKWRPKNFTELVGQEHVVRAISNALSQNRLHHAYLLTG
                     TRGVGKTTLARIIAKALNCETGITATPCGKCAACVEIDGGRFVDLIELDAASNTQVDN
                     MRELLENALYSPASGRFKVYIIDEVHMLSRSAFNAMLKTLEEPPAHVKFILATTDPQK
                     IPVTVLSRCLQFNLKQIPLPQIRGQLENVLGKEGVPFDVPALNLLARAAQGSMRDALS
                     LLDQAIAHGAGRVEEAAVRDMLGAVDRGYLFAILHALTRSDGPGLLAEAERMSARSLS
                     LETALQDLATLLHRIALAQTVPDALASDEPERDALIALAGKFAPEDVQLLYQIAIRGR
                     DELGLAPDEFAGFTMTLMRMLAFVPVEAAGSVPTGVSNRP"
     gene            complement(1252..1350)
                     /gene="ffs"
                     /locus_tag="VN496_14505"
     ncRNA           complement(1252..1350)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN496_14505"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1370..>2530)
                     /locus_tag="VN496_14510"
     CDS             complement(1370..>2530)
                     /locus_tag="VN496_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXF18217.1"
                     /translation="RNTKDNTPAVVHMEVVPGDTVEVKIAAKGGGSENKSKFVMLNPS
                     DSIVDWVLKTVPTMGAGWCPPGMLGIGIGGSAEKAMVLAKEALMEPIDMHELKQRGPK
                     NRIEELRVELCEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASLPIALIPNCAATRH
                     VHFTLDGSGAARLEPPNLDRWPDVQWTPAASSRRIDLAKVTREQIASWKPGESLLLNG
                     KILTGRDAAHKRIQDLLSKGERLPAGVDFTNRFIYYVGPVDAVRDEVVGPAGPTTATR
                     MDKFTETMLGQTGLLGMIGKGERGPVAIEGIRKHKAVYLMAVGGAAYLVSKAIKSSRV
                     VAFADLGMEAIYEFEVKDMPVTVAVDVNGTSVHQTGPREWQSKIGKIPVAVL"
BASE COUNT          494 a          814 c          689 g          533 t
ORIGIN      
        1 tgcggccgat ttgatacgcc ggtaggaacg ctgcctgcag cctccaccgg aacaaaggcc
       61 aacatgcgca tcaaagtcat ggtaaagccg gcgaattcat cgggcgcgag gcccagttcg
      121 tccctcccgc ggatggcaat ctggtagagc aattgcacgt cctccggggc gaacttgccg
      181 gccagcgcaa tcagtgcgtc gcgctctggc tcgtcgctgg caagtgcatc gggcacagtc
      241 tgcgccagcg cgatcctgtg cagcaacgtc gcgagatcct gcagcgcggt ctccagcgaa
      301 aggctgcgcg cactcatgcg ctccgcttcg gcaagcaatc cgggaccatc actgcgagtt
      361 aacgcatgca gtatcgcgaa cagatagcct cgatccacgg cgcccaacat gtctcgcacc
      421 gcggcctctt ccactcggcc cgccccgtgc gcgattgcct ggtccagcag ggacagtgca
      481 tcccgcatgc tgccttgtgc cgcgcgagcc aacaggttca gcgccgggac atcgaatggc
      541 acgccttcct tgccgagcac attttccagc tggccgcgga tttgcggcaa tggaatctgc
      601 ttgaggttga actgcagaca gcgtgacagc acagtcaccg gaatcttctg cggatcagtc
      661 gtcgccagaa tgaatttgac gtgcgccggc ggttcttcca gcgttttcag catcgcgttg
      721 aacgccgagc gcgagagcat atgcacttcg tcgatgatgt agaccttgaa gcgcccgctg
      781 gcaggcgagt acagcgcgtt ctccagcaac tcgcgcatgt tatcgacctg cgtgttcgat
      841 gcggcatcga gttcaatcag atcgacgaaa cgcccgccat ctatttcgac acacgccgcg
      901 cacttgccgc acggcgttgc ggtaatgccg gtttcgcagt tcagcgcctt ggcaataatc
      961 cgcgccagcg tggttttacc cacaccgcgg gtgccggtca gcaggtaggc atggtgcagc
     1021 cggttctgcg acagcgcatt gctgatcgcg cgcaccacgt gttcctggcc gaccaactca
     1081 gtgaaatttt tcgggcgcca tttacgcgcc agcgctagcg tcacggcgtt ctccggccac
     1141 cggtcgattc cgcggcggca ctgccgttga ggcaaccgct caatgattca cccaggttca
     1201 tccaatacaa aatatcatcc ccctcaacca attcgccaat ttcaaaaatg aggtggcgag
     1261 ccttaccccc ggcacttgca gaaaatagct gtggctgctt ccttccggac ctgaccaggt
     1321 tcactaccat gcaatgcggg gaggcccgcc attgaaaaaa agtgcagttc tacaggactg
     1381 cgaccggtat cttgcctatc ttcgattgcc attcgcgcgg gccggtttgg tgcaccgacg
     1441 tgccatttac atcaaccgca acggtcaccg gcatatcctt gacttcaaat tcgtagatcg
     1501 cttccatgcc gaggtccgca aacgcgacca cgcgggagga tttgatcgct ttcgatacca
     1561 ggtaagcagc accgccgacc gccattaaat aaactgcctt gtgcttgcgt ataccctcga
     1621 tcgccacggg accgcgctcg cccttgccga tcatcccgag cagacccgtt tgtcccagca
     1681 tggtttccgt aaacttgtcc atgcgcgtcg ctgtcgtagg tccggccggg ccgaccactt
     1741 catcgcgcac cgcatcaacc ggccccacgt aataaatgaa ccggttcgtg aaatcaacac
     1801 ccgcgggaag ccgctcgcct ttgcttagca agtcctgaat ccgcttatga gctgcatccc
     1861 ggccggtcaa tattttgcca ttcagcaaca gcgactcacc cggcttccag ctcgcgatct
     1921 gttcacgcgt aaccttggcc agatcgatac ggcgtgatga tgccgccggc gtccattgga
     1981 cgtcaggcca ccggtcgaga ttcggtggtt ccagccgggc ggcgccgctg ccatcgagcg
     2041 tgaagtgcac atggcgggtt gccgcgcagt tcggaatcaa ggctatcggc agcgaagcgg
     2101 catgcgtggg atagtcgagg atttttacgt ccagcacggt ggtcaagccg ccgagccctt
     2161 gcgcgccaat acccagcgca ttgacctttt cacacagttc gacacgcaac tcttcgatcc
     2221 tgttcttcgg accgcgctgc ttcaattcat gcatgtcgat cggttccatc agcgcttcct
     2281 tggccaggac catggctttc tcggccgagc ctccgatacc gataccgagc atgccgggcg
     2341 gacaccagcc cgcgcccatg gttggcacgg ttttcagcac ccagtcgacg atactgtcgg
     2401 aagggttcag catcacaaac tttgatttgt tctctgagcc tccccctttc gcagcaattt
     2461 tgacttcaac cgtgtcaccg ggcacgactt ccatatggac aaccgccggg gtgttgtctt
     2521 tggtgtttct
//
LOCUS       DATMZQ010000457         4185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1579640, whole genome shotgun sequence.
ACCESSION   DATMZQ010000457 DATMZQ010000000
VERSION     DATMZQ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579640"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VN496_14515"
     CDS             <1..514
                     /locus_tag="VN496_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HXF18218.1"
                     /translation="RSHLLHATIGALKASGLVCICVSRGDSISASPQLRALAVDDVET
                     LNASNQRAFFNLYNQLRERAGIVLAAGNAPPAQLKLRPELMTRLAWGLIYQVQALTDE
                     EKAQALKRHAASRSFDLPDGVIDYLLRHMSRDLPSLMGMLDALDRHSLENKRPITLPL
                     LKELLRPEAN"
     gene            709..1365
                     /locus_tag="VN496_14520"
     CDS             709..1365
                     /locus_tag="VN496_14520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXF18219.1"
                     /translation="MRLALFDLDNTLLAGDSDFEWAQFLIEKEVLDREVYEARNRDFY
                     EAYKAGTLDIHEFLDFQLKPLARHPRKLLEQWHGEFMQKKIMPIITRKARDLVDSHRD
                     DARVVITATNRFVTAPIAREFGIENLIATEPEERAGEFTGRVKGVPSFREGKVERLRT
                     WMREHGIQWDRVEETWFYSDSLNDLPLLSLVSHPVAVDPDETLKEHALMHDWRVISLR
                     "
     gene            1396..2376
                     /locus_tag="VN496_14525"
     CDS             1396..2376
                     /locus_tag="VN496_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXF18220.1"
                     /translation="MSRALAVLLLALVATSAVLAGVAVGSVPIPLDAIVLALIHPGQD
                     AVSEIVWRLRLPRVLAAFCCGALLALAGTLMQVLLRNPIADPYVFGISGGAALGVMFG
                     MLLGVGAFMQTGLGLIGALLALILVFGLSLRAGNWNPYRLLLTGVVIAAGFNALLSLI
                     LVLAPQTAIKGMLYWLMGDLGYAGDPLPALLVLALLVAVVMPFTRAMNVLAIGRLKAR
                     SLGVAVSGIEAVLFGVASVAAVTAVLIGGTVGFIGLIVPHLVRLLGLADHRSLLPSAV
                     LAGGGFLTIADTIARSAWAPLQFPVGVLTALLGVPILLLLLSGKNKDAAH"
     gene            2363..3151
                     /locus_tag="VN496_14530"
     CDS             2363..3151
                     /locus_tag="VN496_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18221.1"
                     /translation="MLRIEDLRLGYPGRTLVEKLSAEFRPGELWAVLGRNGSGKTTLL
                     HALAALLQPLAGRVMLGETMVSALPRRMLARHIGLLLQEESTEFWGSVQDYVLLGRYP
                     HAAGVFGYSEDDQRIAQCELEAAHLGELRQRAYASLSGGERQRARAAALFAQRPVIYM
                     LDEPLQHLDLPHQVAVLERLSAQAQERGAIVIMALHDLIFAARYCSRFLLLYGDGRYV
                     LGTSDHTMTANLLSELYAFPLTGISNDGEQLFLPARQARKGPHV"
     gene            3191..>4185
                     /gene="pcnB"
                     /locus_tag="VN496_14535"
     CDS             3191..>4185
                     /gene="pcnB"
                     /locus_tag="VN496_14535"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HXF18222.1"
                     /translation="MIRKLLRRVFSRGSSSSAGLQVIPRSKHGLTKERISASARRIAT
                     GLQQKGFAAYVVGGAVRDLLLGLEPKDFDMATDATPEQVRSVFRRSRIIGRRFRLVHV
                     MFGEDTVEVSTFRARQGDDQEDQRADEHGRIVRDNVFGTQEEDATRRDFTINALFYDP
                     TTEQILDFCGGLDDLKARRLRMIGEPDQRYREDPVRMLRAVRLSAKLGLKIEFETRAP
                     IKELAPLLANVPSSRLFEEMMKLLFSGHATACLLQLRQEGLHHGLLPMLDVILEQPMG
                     ERFVMMALKSTDERVRAQKPVSPGFLFAALLWHEVLAAWKQAQEEGLNPLPALFKA"
BASE COUNT          811 a         1132 c         1241 g         1001 t
ORIGIN      
        1 acggtcgcat ttgttgcatg ccactattgg tgcgctgaag gcgtccggcc tggtttgcat
       61 ttgcgtgtcc cgtggcgatt cgatttctgc atcgccgcaa ttgcgcgcac ttgcagtgga
      121 cgatgtcgaa acgctgaatg ccagtaatca acgcgcgttt ttcaatttgt acaatcagct
      181 gcgggaacgt gccggcatcg tcttggctgc tggcaacgct ccgccagcgc aactgaagtt
      241 gcgtccggaa ctaatgacgc gattggcttg ggggctgata tatcaggttc aggcactcac
      301 cgacgaggag aaagcgcaag cgctcaagcg ccatgctgcc tcacgctcat tcgatcttcc
      361 tgatggagtg atcgattatt tattgcgcca tatgagccgc gacctgcctt ctttgatggg
      421 tatgctggac gcgctggacc ggcactcgct cgaaaacaag cggcctatca cattaccact
      481 gctgaaggaa ttgctgcggc ctgaagccaa ttgaacccgg tcggtgcacg gaccaggaac
      541 ggttttttgc ggcctttaag ttatactgct tctgcttgcc tcaatactcc cgccgctctt
      601 gttcaacatc attactgtgc ttttgcattg ttgattcaaa aaacacgccc aggttcggcg
      661 agcccgacct acagccacaa aaggctaaat cttcctctgc ccaagatcat gcgtcttgca
      721 ttattcgacc tggataacac gttgctcgct ggagacagcg atttcgagtg ggcgcaattt
      781 ctgattgaga aggaagtgct cgaccgagaa gtttacgaag cgcgcaatcg tgatttttac
      841 gaagcgtaca aggccggcac gctggatatc cacgaatttc tggactttca attgaagccg
      901 ctggcacgcc atccgcgcaa actgctcgag cagtggcatg gcgaattcat gcagaaaaaa
      961 ataatgccca tcatcacgcg taaggcccgt gacctggttg acagccatcg cgatgatgcc
     1021 cgggtggtaa ttactgccac aaaccgtttc gttaccgcgc caatcgcgcg tgaattcggt
     1081 atagagaacc ttatcgcgac cgaaccggag gaacgcgccg gtgaatttac cggccgcgtg
     1141 aaaggcgtac cgtcattccg tgaaggcaag gtggagcgac tgaggacgtg gatgagagag
     1201 cacggaatac aatgggatcg tgtcgaggaa acctggtttt atagcgattc tctgaacgac
     1261 ctgccgctgc tgtcgctggt cagccatcca gtagcggtag atccggacga gacgctcaag
     1321 gagcatgcgt taatgcacga ctggcgtgtt atcagcctgc gttaagggat cgggtggcgc
     1381 gggccggaac gaataatgtc gcgcgccctg gcagttttgt tacttgcgtt ggttgccaca
     1441 tctgcagtgt tggctggcgt ggccgttggc agcgttccca tccctcttga cgcaattgtc
     1501 ttggctttga tccatccggg gcaggatgca gtttccgaaa tcgtgtggcg actgcgcttg
     1561 ccacgcgtcc tggccgcctt ttgctgtgga gcgttgcttg cgctggcggg cacgctgatg
     1621 caagtcctgc tgcgcaatcc gatcgccgat ccttacgttt ttggcatatc cggtggcgca
     1681 gcactgggcg tcatgtttgg catgttgctt ggtgttggcg cattcatgca gactggactg
     1741 ggattgattg gcgcactgtt ggcgttaatc ctcgtgtttg gcctgagctt gcgcgctgga
     1801 aattggaatc cttatcggct gctattgacc ggtgtggtaa tcgcggctgg gttcaatgca
     1861 ctgctcagcc tgattctcgt cctggcgcct cagactgcaa tcaaaggaat gttgtactgg
     1921 ctgatggggg accttggata cgccggcgat cctttgccgg cgcttttggt tttggcgcta
     1981 ttggttgcag tggtcatgcc gtttacccgt gccatgaatg tgttggccat cggacggttg
     2041 aaggcgcgct cactgggagt agcggtgagt ggcatcgaag cagtcctatt cggtgtggct
     2101 tccgtcgccg ctgtgactgc ggtactgatt ggaggcaccg tggggtttat cggcctgatc
     2161 gttcctcatc ttgtgcgctt gcttggcctg gcggatcatc gctcactgct tccgtcggct
     2221 gtcctggctg ggggcggctt tctgactatt gcggacacga ttgctcgcag tgcatgggca
     2281 cctctgcagt tcccggtcgg ggttctaacg gcgctgttgg gcgtacccat tcttcttctg
     2341 ctcctctcgg gaaaaaacaa ggatgctgcg cattgaggat ttgcggctgg gctatccagg
     2401 ccgcacgctg gtcgaaaaat tgtcggccga attccgcccc ggggaactgt gggcggtact
     2461 aggacgcaac ggcagcggca aaacaaccct gttgcatgca cttgctgcat tgttgcaacc
     2521 gcttgctggc cgcgtaatgc tcggtgaaac gatggtcagt gccttgccga ggcgcatgtt
     2581 ggcccgccac atcgggttac ttctgcagga agaaagcact gaattttggg gtagcgtcca
     2641 ggattacgtt ttgctgggtc gttacccaca tgccgctgga gttttcggat attccgagga
     2701 cgatcagcgc atcgcgcaat gcgaacttga agcggctcat ctcggtgagc tgcgacagcg
     2761 ggcctacgct tctctctcgg gcggtgaacg ccagcgcgca cgagctgctg cgttgtttgc
     2821 gcaacgccct gtgatttata tgcttgatga gcctctgcaa catcttgacc tgccgcacca
     2881 ggttgcggtg ctggaacggc tatcggcaca agcgcaagag cgtggtgcga tcgtcatcat
     2941 ggcattgcac gatctgattt ttgccgcgcg ctattgcagc cgtttcctgc ttctttatgg
     3001 ggatgggcgc tacgttcttg gaacttcaga ccacacaatg actgccaatt tactcagtga
     3061 actgtacgcc tttcccttga ccggaataag caatgacggt gagcaactgt tcctgccagc
     3121 ccggcaagcg cgcaaagggc cgcatgtata atcgaagctt ttacgtccca gcggaattcc
     3181 gcagtacttg atgattcgga aacttcttcg ccgggtattt tcccgcgggt cttcgtcatc
     3241 cgccggtctt caagtcatcc cgcgcagcaa gcatggcctg acaaaggaaa ggatcagtgc
     3301 cagcgcacgg cgcattgcca cggggttgca gcaaaaaggt ttcgccgcct atgttgtcgg
     3361 cggtgcggtc cgcgatctgc tgcttggact ggaaccgaag gatttcgata tggcaacgga
     3421 cgccacacca gaacaggtgc gaagcgtatt tcggcggtcg cgcatcattg gacggcggtt
     3481 ccggctggtt cacgtaatgt tcggcgaaga taccgtggaa gtatcgacct tccgcgcccg
     3541 ccagggcgac gatcaggagg atcagcgcgc cgatgagcat ggccgcattg tccgcgacaa
     3601 cgtgttcggc acccaggaag aggatgccac ccggagggac ttcacgatca atgccctgtt
     3661 ctacgatcct acaacggagc agatccttga tttctgtggc gggctcgatg acttgaaagc
     3721 ccgccgcttg cgcatgatcg gggagccaga ccaacgctat cgcgaagatc cggtgcgcat
     3781 gctacgtgcc gtgcggcttt ccgcaaaact cgggttgaaa atagaattcg aaacccgcgc
     3841 tccgatcaag gaactcgcgc cgctgcttgc gaacgttccg tcttcgcgac tgttcgaaga
     3901 gatgatgaag ttgctctttt caggacacgc cactgcctgt cttctgcagc tacggcagga
     3961 agggctccat catggccttt tgcccatgct cgatgtgatc ctcgagcagc cgatgggaga
     4021 gcgcttcgtc atgatggcgc ttaaaagcac cgatgagcgc gtgcgtgcgc aaaaaccggt
     4081 atcgcccggt ttcctgtttg ccgccctgct ttggcatgaa gtgctcgccg cgtggaagca
     4141 ggcacaggag gaggggctca atccattgcc ggcgctgttc aaggc
//
LOCUS       DATMZQ010000458         4255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1579697, whole genome shotgun sequence.
ACCESSION   DATMZQ010000458 DATMZQ010000000
VERSION     DATMZQ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579697"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VN496_14540"
     CDS             complement(<1..464)
                     /locus_tag="VN496_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXF18223.1"
                     /translation="MTRVCVFCGSNPGLRADYGIAVQGLAIALVRSGFDLVYGGGNVG
                     LMGQLADAMLHAGGQVIGVIPRALCDKEVAHHGVTELRIVETMHQRKAMMNDLSDAFI
                     ALPGGYGTLDEFFEILTWAQLGIHAKPCGLLNVAGYYDSLLAMLDRAVAEGF"
     gene            complement(466..987)
                     /locus_tag="VN496_14545"
     CDS             complement(466..987)
                     /locus_tag="VN496_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18224.1"
                     /translation="MIANRSLALALATLCLTAMAQTTAQDVRKQWPDLRYLDPGQVRE
                     LPAPVRNDLEKLGCRIPKFLKWDARHNVIQGQFFKAGQHDWAVLCETRDKSGILLYPG
                     GAATGLPMLYAQPIDPARMIHTVSPFVLQKRAIRDHPETPPPSFDHDGIEDGAVQKTG
                     RVLYFQDDEWTEL"
     gene            complement(1052..1768)
                     /locus_tag="VN496_14550"
     CDS             complement(1052..1768)
                     /locus_tag="VN496_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18225.1"
                     /translation="MLELKNVASYYGSVQALRRVSLRVEQGEVVTLLGANGAGKSTTL
                     RTISGLLRPTNGEVWFQGQPIHGMSTEKIVRLGISHVPEGRRIFPGLTVRENIMLGTS
                     NRGRLHKKQMDEEVAEMFEIFPVIKPFEHALGWTLSGGQQQMLAVARGLMAKPKLLLL
                     DEPSLGLAPVVVQQLFATIRVIHARGTTILLVEQNANMALSVANRGYVLETGEMKVEG
                     SPRELLDNEQVRAAYLGGHG"
     gene            complement(1761..3560)
                     /locus_tag="VN496_14555"
     CDS             complement(1761..3560)
                     /locus_tag="VN496_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXF18226.1"
                     /translation="MSTQILEIPRKRRLSLWPLLVFAVAIAVPLLPYYANVSHFVLSM
                     FMQATTYAIAVLGMVVVLGYTGQINLAQAAFFGFGAYGVALGTVSYGLSFWTALALGI
                     GIAGLAGGILGLTTLRLGGHYLAMITISFQQIFDLVAVNWIEVTHGPDGIAGIGRPSL
                     FGHELADDRAYLLLCSAVLYAMMAAVWYLPQTRLGRAMRGVRENELAAEVVGVHTLRT
                     KVIAFTLCAALGGVGGGLYAGGFAYISPDNFNFQRAVEFLSMVLLGGAQSPFGGALGT
                     TLLILLPEWLKEMPPSLQFIKDVYLAIYGLAVILIMVFMPEGIWGLLRRSWMKLSRDR
                     AVDTSGIKPLNLDIAVTEATPLLRLEGLHKHFGGLRAVDGIDLQVARGTVHALIGPNG
                     SGKTTTLNVVNGIYKPTGGRIIVDGHDVTPMSPHQRAGYGVGRTFQNIRLFPSMSALE
                     NVIVGAQRDNNPIEPGAEALHARAMSALQFVGLEGMANAIVRGLPYGHQRLVEIARAL
                     AGHPKLLLLDEPAAGLNQTEKMELVELLKKLRSGHGLTVFLIDHDMGLVEKVSDRITV
                     LNFGKKIAEGTPQEVLRHPDVIAAYLGDSNAAA"
     gene            complement(3557..>4255)
                     /locus_tag="VN496_14560"
     CDS             complement(3557..>4255)
                     /locus_tag="VN496_14560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF18227.1"
                     /translation="MPYWLSFLLALIGMGVFGLVFNYGVYFPLRNRGFLPVIISTLGA
                     SIFLQNSVLALFGAQPKRMDNVIPGGIQIGSVFLDSQYLVILGVTMVMVTFQYFFFEH
                     TMLGKKLQATSQDKDMARLLGIPVALMIAITFVYSAALGGLAGVLVGPVLFVSIGMGS
                     IIALKAFAATIIGGFGDVKGAIVGGLFIGVIESFAAFYISVPYKDAYAFLMLFLVLIF
                     RPQGFFGEKVSEKA"
BASE COUNT          919 a         1312 c         1192 g          832 t
ORIGIN      
        1 aaaaaacctt cggcgactgc gcggtccagc attgccagca ggctgtcgta atatcctgcc
       61 acattcagca gcccgcacgg ctttgcgtgg ataccaagtt gcgcccaagt cagaatttcg
      121 aagaattcgt ccagcgtacc gtagccgccc ggtagtgcga tgaaggcatc ggacaaatca
      181 ttcatcatgg ctttgcgctg atgcatggtc tcgacgatgc gcagttcggt gaccccgtgg
      241 tgtgcaacct ccttgtcaca caatgctctt gggatcacgc caatgacctg gcctccggca
      301 tgcaacattg catctgcaag ctggcccatg aggccgacat ttccgccgcc gtagaccaga
      361 tcaaatccgg agcgaaccaa agcaattgcc agtccttgca cggcgatccc gtagtcagcg
      421 cgcagcccgg gattggagcc gcaaaataca cacacgcgtg tcacttcaca attccgtcca
      481 ttcgtcgtcc tgaaagtaaa gtacgcggcc agtcttctga acggcgccgt cttcaatgcc
      541 gtcgtgatca aaggatggcg gcggtgtttc gggatgatcg cgtatcgcgc gtttttgcaa
      601 gacgaacggg ctcacggtat gaatcatgcg ggcgggatca atcggttgcg catacaacat
      661 cggaagcccc gtggctgcgc ctccgggata aagcaggatt ccgctcttat cccgcgtctc
      721 gcacaatacg gcccagtcat gctgtcccgc tttgaagaat tgcccctgga tgacgttatg
      781 tcgcgcgtcc cacttcagga attttggaat gcggcagccg agcttttcga gatcgttacg
      841 cactggggca ggcagttcac gaacctggcc gggatctagg taacgcaggt ccggccactg
      901 cttgcgcacg tcctgtgccg tagtttgtgc catggccgta aggcaaagcg tggcgagcgc
      961 cagagcgagc gatctattgg caatcacggc gtttctcctg tgcaaccgcg gaggatccag
     1021 tgtaatgccg cgcaaaaaac tgcgtcgccg ttcagccgtg gccgccgaga tacgccgccc
     1081 gaacctgctc gttgtccagc agttcccggg gcgaaccttc cactttcatt tcgccggttt
     1141 caagcacgta gccccgattg gcaacggata acgccatgtt cgcattctgc tcgaccagca
     1201 gaatggtcgt gccccgcgca tggatgaccc ggatggtcgc aaatagctgc tgcacgacga
     1261 ccggcgcaag gccaagcgag ggttcgtcaa gcaacaataa ctttggtttg gccatgagcc
     1321 cgcgcgccac cgcgagcatt tgctgctggc cgcccgaaag cgtccagccc aaagcatgct
     1381 cgaatggttt gatgaccgga aagatctcga acatttcggc gacttcttca tccatttgct
     1441 ttttgtgcag ccgcccgcgg ttcgatgtgc cgagcatgat gttctcgcgc acggtgaggc
     1501 ccgggaaaat cctgcgccct tccgggacgt gcgaaatgcc cagccgcact attttttcag
     1561 tcgacatccc gtggatgggc tgtccctgga accatacttc gccgttcgtc ggcctcagta
     1621 gcccggaaat ggtacgcaat gtcgtgcttt ttccggcgcc gttggcgccg agtagcgtga
     1681 cgacttcgcc ttgttcgacc cgcaggctta cgcgcctgag cgcctgcacg ctgccgtagt
     1741 agctggcaac gttttttagc tcaagcagcg gcattgctgt ctcccaggta cgcggcaatt
     1801 acgtccgggt gccggagcac ttcctgcggc gtgccttcgg cgatcttctt gccgaaattc
     1861 agtacggtaa tgcggtcgga taccttttcc accagcccca tgtcatggtc gatcaaaaac
     1921 accgtcaatc cgtggccgct gcgcaatttc ttcagcagtt ccacgagttc cattttctcg
     1981 gtctggttca atccggcagc aggttcgtcc agcagcagca gcttcggatg cccagcgagc
     2041 gcgcgcgcaa tttcaaccag gcgttgatga ccgtacggca gcccgcgcac gatggcattt
     2101 gccatgcctt ccagtcctac gaattgcagc gccgacatcg cgcgcgcgtg cagcgcttcg
     2161 gcgccgggtt caatcggatt attgtcacgc tgcgcgccca caatcacgtt ttccagtgcc
     2221 gacatgctcg ggaacagccg tatgttctgg aatgtgcggc caacgccata tccggcgcgc
     2281 tgatgcggcg acatcggcgt gacatcgtgg ccgtcgacga tgatgcggcc gccggtcggt
     2341 ttataaatgc cattaacaac gttcagtgtg gtcgtcttgc cggagccgtt cggaccaatc
     2401 aaggcatgca ccgttccgcg tgcgacttgc aggtcgatac cgtcgaccgc gcgcaggccg
     2461 ccaaaatgtt tatgcaggcc ttcgagccgg agcagcggcg tcgcttcggt cactgcgata
     2521 tcaaggttca ggggcttgat gccagacgta tcaaccgctc tatctcgact cagcttcatc
     2581 cagcttctgc gcagcagtcc ccagatgccc tcgggcatga ataccataat cagaatgacg
     2641 gccaatccgt agatcgcgag atacacatcc ttgatgaatt gcaggctcgg cggcatttcc
     2701 ttcagccatt ccggcagcaa aatcagcagc gtggttccga gcgcaccacc aaacggcgac
     2761 tgcgctccgc cgagcagcac catcgataga aactccaccg cccgctggaa gttgaaattg
     2821 tccgggctga tgtacgcaaa gcccccggca tagagcccgc ctccgacgcc accgagcgcc
     2881 gcgcacagcg tgaaagcaat gactttcgtt ctcaacgtat gcacgccaac gacttccgca
     2941 gcgagttcgt tttcgcgcac gccacgcatc gcacgcccga gccgcgtctg cggtagatac
     3001 cagaccgcgg ccatcatcgc gtagagcacc gctgagcaca gcagcagata ggcgcggtcg
     3061 tccgcaagct cgtgtccgaa cagcgacggg cggccgatgc cggcaatgcc gtccggccca
     3121 tgcgttacct cgatccagtt gaccgccacc aagtcaaaaa tctgctggaa gctgatggta
     3181 atcatggcca gataatgccc gccgaggcgt agcgtggtca ggcccagaat accgccggca
     3241 agtccggcga tgccgatgcc gagcgccagc gcagtccaaa acgacagacc gtagctgacg
     3301 gtgcccaatg ccacgccata cgcaccaaaa ccgaagaacg cggcctgggc aagattaatc
     3361 tggccggtgt aaccgagcac caccaccatg ccgagcactg cgattgcata cgtcgttgcc
     3421 tgcatgaaca tcgacaacac gaaatggctg acattggcgt agtaaggcag caacggcact
     3481 gcgatggcga ctgcaaagac gagcaacggc cacagcgaca gtcggcgctt gcgcgggatt
     3541 tccaggattt gggtgctcat gctttttcgg acaccttctc gccgaagaag ccctgcggcc
     3601 ggaatatcaa taccaggaac agcatcagga aggcgtatgc atccttatag gggaccgata
     3661 tgtagaacgc agcgaagctt tcgatgaccc cgatgaacaa gccccccacg atggcaccct
     3721 tgacatcgcc gaaaccgccg atgattgtgg cggcgaatgc cttgagcgcg atgatcgatc
     3781 ccatgccgat cgatacgaac agcaccgggc ccaccagcac gccggccagt ccgccgagcg
     3841 cggcgctata aacaaaagtg atggcgatca tgagcgcgac cgggatgccg agcagccgcg
     3901 ccatgtcctt gtcctgcgac gtcgcctgca gtttcttgcc gagcatcgtg tgttcgaaga
     3961 aaaaatactg gaatgtcacc atgaccatcg tcacgccaag gatgaccaaa tactggctat
     4021 ccagaaacac ggagccgatc tggatgccgc cgggaatcac attgtccatg cgtttcggct
     4081 gcgcgccgaa cagtgcgagc acactgttct gcagaaagat cgatgcaccg agcgtggata
     4141 tgatgactgg cagaaaaccg cgattgcgca gcggaaagta cacgccgtaa ttgaagacca
     4201 ggccgaacac gcccatgccg atcagggcca gcaggaaaga cagccaatac ggcat
//
LOCUS       DATMZQ010000459         4031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2269568, whole genome shotgun sequence.
ACCESSION   DATMZQ010000459 DATMZQ010000000
VERSION     DATMZQ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269568"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VN496_14565"
     CDS             <1..265
                     /locus_tag="VN496_14565"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HXF18228.1"
                     /translation="YARAFGTVGQDATLTLYRKAAEAATRSGLGINAGHDLNRNNLPL
                     FLSSVPGVLEVSIGHALISDAIEFGMISTVALYLAAIRQGGST"
     gene            262..639
                     /gene="acpS"
                     /locus_tag="VN496_14570"
     CDS             262..639
                     /gene="acpS"
                     /locus_tag="VN496_14570"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXF18229.1"
                     /translation="MIVGVGIDIVDVSRIERLLKQYGGQFAGRILCGTEREGFDASTR
                     QAWFLANRFAAKEALSKALGTGLRYPVTLQAISVTNDSLGKPMFGFHGPLPQYLASRG
                     VRHSHLGLSHEKGMACAMVVLEQ"
     gene            641..1723
                     /gene="nagZ"
                     /locus_tag="VN496_14575"
     CDS             641..1723
                     /gene="nagZ"
                     /locus_tag="VN496_14575"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HXF18230.1"
                     /translation="MTTRTTSLGLVMLDIEGKTLNDADRKRLSHPLTGGVILFSRNYE
                     SPRQLEELADEIHRLRNPPLLIAVDHEGGRVQRFREGFTRLPPMRSIGSLWNQHPLRA
                     RHLARDAGYILASELRAHGVDLSFTPVLDLDFGNSAVIGDRAFHAEPHAVAELAGALV
                     EGLTLAGMGSCGKHFPGHGFVAADSHTEVPVDSRTYEQIEAADLIPFRALMAHGLSAV
                     MPAHVIYPAVDDRPAGFSKKWLAQILRTEMKFEGVIFSDDLSMEGARVAGDIVGRAEA
                     ALDAGCDMVLVCNRPDAADELLRRLQRKMSPASLLRVARLHGRASPPTMTALKATEIY
                     GRAVRAIGAIGQESGDLLAGNIGERG"
     gene            1716..2678
                     /locus_tag="VN496_14580"
     CDS             1716..2678
                     /locus_tag="VN496_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXF18231.1"
                     /translation="MAKHTHDHGHQGTRQTLLFALAITLAYALVEVVGGAIFHSLALM
                     SDAGHMVSDALALGLAAFASWLGSRPSGSRHSYGFARAEVVAAFVNGLAMLLVVVLIA
                     VEAISRLLHPEPVSGFGVMLVAIFGLVANLFVAFLIGQGERNLNTRAVLLHIVGDLIG
                     SVAAITSGAVIYYTGWQPIDALLSLVIAMLILGSTVRLLLDALHVLMEGVPVGLTIED
                     VGRALGELRGVLAVHDLHIWNISSGQKALSAHVDLENLDSWAALLESARQMLRHRFGI
                     AHVTLQPEPAGGINPGYRARVKILPHQREHVRDDGKRRRHSDEH"
     gene            2685..3329
                     /locus_tag="VN496_14585"
     CDS             2685..3329
                     /locus_tag="VN496_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXF18232.1"
                     /translation="MTAFCVECRRCGRLAGFLDDVRAEYPHYHARPVPPFGDASPRLL
                     IVGLAPGMHGANRSGRPFTGDHAGVLLYQTLHKFGFADRPASISSDDGLSLIGCRITN
                     AVKCLPPENKPTGDEVRRCNTYLASEIASLEKNSVLLALGGIAHKAVLMAAAEKTSAH
                     SFAHGARHRLHNGLMLFDSYHCSRYNTQTRRLTASMFEQVFAEIRRSIEHPVIQ"
     gene            3378..>4031
                     /locus_tag="VN496_14590"
     CDS             3378..>4031
                     /locus_tag="VN496_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXF18233.1"
                     /translation="MAFDPGEIIANLPHLPGVYRMLNAADEVLYVGKARDLKKRVASY
                     FQKTASLSPRIQLMVGQVQGLETTVARSEAEALLLENNFIKSLTPRYNILYRDDKSYP
                     YLVLTAHRFPRLGFHRGSLDKTNRYFGPFSNAGAVRESIRLMQKIFRLRTCEDTVFAN
                     RSRPCLLYQIKRCTGPCAGLIDDANYAEDVRSAELFLQGREDEAVSRLEERMRRAADE
                     "
BASE COUNT          806 a         1115 c         1205 g          905 t
ORIGIN      
        1 ctatgcacgt gcttttggga cggttggcca ggacgctacg ctcacccttt accgcaaagc
       61 cgccgaggcc gccacacgta gcggtttggg tatcaacgcc ggacacgatc tgaatcgcaa
      121 caacttgccg ctattcctct ctagcgtacc cggtgttctt gaggtatcca tcggccacgc
      181 gctcatctcc gatgccattg aattcggaat gatctccact gtcgcgcttt atcttgcggc
      241 gatccgccag ggaggaagca cgtgatcgtc ggggtcggca tcgatatcgt ggatgtaagc
      301 cgcattgaac ggctgttgaa gcaatacggt ggtcaattcg ccggacggat tctgtgcggg
      361 acggaacgcg aaggattcga cgccagtacc cggcaggcct ggttccttgc gaaccgcttt
      421 gccgccaaag aggctttgtc aaaagccttg ggcaccggct tgcgttatcc ggtaacgctg
      481 caagcgatca gtgtcaccaa cgacagtctc ggcaagccga tgttcggatt tcacggcccg
      541 ttgccgcagt atctcgcgag ccgcggtgtg cggcattctc acctcggctt gtctcacgaa
      601 aaaggaatgg catgcgcaat ggtcgtattg gagcaatgac atgacgacgc gcactacgtc
      661 cctgggtctg gttatgctcg acatcgaagg caagacgctc aacgatgccg accgaaaaag
      721 gctgagccat ccgctcactg gcggcgtaat cctgttttcc agaaactatg aatctccgcg
      781 ccagctcgag gagttggcgg acgaaataca tcgcctgcgc aatcctcctt tgctcatcgc
      841 agttgaccat gaaggtgggc gtgtgcagcg ctttcgagag ggcttcacgc ggctgccgcc
      901 gatgcgctca attgggagtc tctggaatca gcatccgctg cgtgcccggc acttggcgcg
      961 cgacgcagga tacattctcg catcagaatt acgggctcac ggagtggatt taagcttcac
     1021 tccggtgctg gacctcgatt ttggcaatag cgctgtcata ggcgaccgtg ccttccatgc
     1081 tgagccgcat gccgtggccg aactggccgg cgcattggtg gagggactta cccttgcggg
     1141 tatgggttcc tgcggaaagc attttccagg tcacggtttt gtcgccgcgg attcgcatac
     1201 cgaggtgccg gtcgatagcc gcacttatga acaaattgag gccgccgatc tgatcccgtt
     1261 ccgtgcattg atggcccatg ggttgtccgc tgtgatgccg gctcatgtca tttacccggc
     1321 tgtagacgat cgtcccgcgg gtttttcgaa gaaatggctc gcgcagatac tgcgtactga
     1381 aatgaagttt gagggtgtca tattcagcga cgatcttagt atggaagggg ctcgcgtagc
     1441 cggggacatc gtcgggcgtg cggaagcagc gctggatgcc ggctgcgaca tggtgctggt
     1501 ctgtaatcgc ccggatgcgg ccgacgaact gttgcgccgt ttacaacgaa aaatgtctcc
     1561 tgcatcgctc ttaagggtgg cgcgccttca cgggcgcgcg tccccgccga ccatgaccgc
     1621 gctcaaggca acagaaatct acgggcgggc cgtgcgcgcc atcggagcaa ttggtcagga
     1681 aagcggcgac ctgctggctg gaaacattgg ggaacgtggc taaacatact cacgatcatg
     1741 ggcatcaggg tacgcggcag acgctgctct ttgccttagc catcacgctg gcctacgcgc
     1801 tggttgaagt tgttggcggg gcaatttttc attcactggc gttgatgagc gatgcagggc
     1861 atatggtttc cgacgcactc gcgctgggac tggcggcgtt cgcgtcgtgg ctgggcagcc
     1921 gtccatccgg cagccggcat tcgtatggct tcgcacgggc ggaagtggtc gctgcgttcg
     1981 tgaacgggct tgccatgctg ctggtcgttg tcctgatcgc ggtggaagcc atttccagat
     2041 tgctccatcc cgagcccgtt tccggcttcg gcgtcatgct cgtggcaata tttgggctag
     2101 tggcaaacct gttcgtcgca tttctcatcg gccaggggga acgcaatctc aacacgcggg
     2161 cggtactgct ccatatagtc ggagacctta tcggttccgt tgccgcaatt acatccggag
     2221 cagtaattta ttacaccggt tggcaaccga ttgacgcgct gttgtccctg gtcattgcga
     2281 tgctgattct gggatcgacg gtgagactat tgctggatgc tttgcacgtt ctgatggaag
     2341 gcgtgcctgt cggtctgacc atagaagatg tcggtagagc gcttggagaa ctgcgtggcg
     2401 tgctcgcggt gcatgacctg catatctgga atatttcttc tggccagaag gcactctccg
     2461 cccacgttga cctggaaaac ctggatagtt gggcggcttt gctggaatcg gcaaggcaaa
     2521 tgttgcgaca ccgcttcggc attgcgcatg taacgctgca gccggaacca gccgggggta
     2581 tcaatccagg ctatcgcgca cgcgtaaaaa tcttgccgca tcaacgagag catgttcgtg
     2641 atgacggtaa gcggcggcga cacagcgatg aacattagcc ggccatgacc gcgttctgtg
     2701 tcgaatgtcg gcgctgcggc cggctggcag gcttcctgga cgacgtgcgc gcagagtacc
     2761 cgcattatca tgcacgcccg gtcccgccgt ttggcgatgc ctcaccccga ttgttgattg
     2821 tcgggctcgc gccgggcatg catggcgcca atcggtcagg tcggcctttt accggggatc
     2881 atgccggcgt gctgctgtat cagacgttgc acaaatttgg ttttgccgac cggccagcct
     2941 ccatatcgag cgacgacggc ctcagcctga tcggctgtcg cattaccaat gcggtgaaat
     3001 gtctgcctcc ggaaaacaaa ccgaccgggg atgaggtcag acgctgcaat acgtacctcg
     3061 caagcgagat cgcaagcctg gaaaagaatt cggttttgct cgcacttggc ggtattgcgc
     3121 ataaggcggt attgatggct gctgcggaaa aaacctctgc tcacagtttc gcgcatggag
     3181 ccagacatcg actccataac ggtctcatgc tgttcgatag ttatcattgc agccgataca
     3241 atacgcagac acgtcggctg accgcatcga tgttcgaaca ggtcttcgcc gaaataaggc
     3301 gttccatcga acacccggtc atccaatagt gtcccatgaa gaatcgtgat ccggttgcgt
     3361 catcagcgac agggcgaatg gctttcgatc caggcgaaat aatcgcaaac cttccgcatt
     3421 tgccaggcgt ttatcgaatg ttgaatgcgg ccgatgaggt gctgtacgtc ggcaaggcgc
     3481 gtgacctcaa aaagcgcgtc gcttcctatt ttcagaaaac ggcttccctc tcgccgcgaa
     3541 ttcaattgat ggtcggccag gtacagggac tggaaaccac ggtagcgcgt tccgaggcag
     3601 aggcgctgct gctcgaaaat aacttcatca aaagtttaac tccacgctac aacatacttt
     3661 acagggacga taagtcttat ccgtacctgg ttctgacggc ccatcgtttt ccgcgtctgg
     3721 gattccatcg cggaagcctc gataaaacca accggtactt tgggccgttt tcgaacgcgg
     3781 gcgcggttcg tgaaagcatc cggttgatgc agaaaatctt ccggctgcgt acctgcgaag
     3841 acacggtatt tgccaatcgt tcccgtccct gcctgctgta ccagatcaag cgctgtaccg
     3901 gtccttgcgc ggggttgatc gatgatgcca attacgccga agatgtcagg agcgccgagt
     3961 tattcctgca gggcagggaa gatgaggccg taagtcgcct ggaggagcgc atgcggcggg
     4021 cggccgatga g
//
LOCUS       DATMZQ010000460         6277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_922578, whole genome shotgun sequence.
ACCESSION   DATMZQ010000460 DATMZQ010000000
VERSION     DATMZQ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922578"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VN496_14595"
     CDS             <1..725
                     /locus_tag="VN496_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXF18234.1"
                     /translation="FTSDLLPADILGVSIFDREVSAFKFHPGPIFTQVILADEINRAT
                     PKAQSALLEAMEEHQVTTEGQTRILPEPFFVIATQNPSHQVGTFPLPESQLDRFLMRI
                     HLGYPDAAAERALLEGRERRDMISELGAALATGDLLELQYKARLIFTAPALLDYVQAL
                     IRYTRQSSDFVNGLSPRAGLAVVNAARAWAFMDDRREVIPEDVQAVLPSVAGHRLSSI
                     TGYSSFSSSQLAERLIAEVPIP"
     gene            734..1750
                     /locus_tag="VN496_14600"
     CDS             734..1750
                     /locus_tag="VN496_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXF18235.1"
                     /translation="MSTPGHDRSIAVPPHSRFRKLKTSSSLRDRVSDWIFPPKGPEIS
                     PIVLGQRRVYILPTGAGLMFGVTILVMLIGSINYNLSLGYVLVFLLSGNGMVSMLHTW
                     RNLARISLRPGKADPVFAGQRAAFRVYIENTSSLPRTSLGVQLTGTEPEFHDVARGSS
                     KLIELKVSTANRGLLYPGRLRIFTTYPVGLFYAWAIVDLGMHCLVYPQPEAGRCILPL
                     ATTSAGEGQAMRAGEEDFAGLRTFRAGDSPRRIAWKAFARTEVLLSKQFSGGAAAEIW
                     LDFRDLPVMEIEAKLSRLTRWVIESEAAGIRYGLRLPGTELDPDSGVPHRERCLQALA
                     LFKS"
     gene            1747..3702
                     /locus_tag="VN496_14605"
     CDS             1747..3702
                     /locus_tag="VN496_14605"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HXF18236.1"
                     /translation="MIARDLSPINLAAILGSLALVIGPHSLHLPGWIPALAAVVLLAR
                     FYFGWRRMKLPNKWLLIALALSSTAAIALSYHTLYGRDVGVALLTVMIALKVMEMTKP
                     RDTMVVVLLAYFLVVTNFFYSQSIPTAFYMLLVVWIITATMIGLQHQAEQPAVVPVLR
                     QAAIMILQGIPVMLTLFVLFPRVQGPLWGLPQINYSAKSGLGESMTPGDLSSLSLSDA
                     VAFRVLFDQPPAAPEQFYWRGPVLWNYDGRTWRAGNYASAGAPYIQRLGPQLRYTVTM
                     ESHDRNWMFAIDMPTSVPDGAYLTTDYQLLSRRIVRDRLRYDMTSSPRYRVGFDESPD
                     ALRLAQRLPSGVSPRARALVAQWRAESIRDRDIVVRALKMFREQPFVYTLSPVPLDAD
                     PVDQFLFETRSGFCEHYASAFTFLMRAAGIPARVVTGYLGGEVNPLDGYVVVRQADAH
                     AWSEVWLPGEGWVRIDPTAAVSPLRVERGLAEALPAIDRPLLARGTFEWLKQARYAWD
                     AAANNWNQWVLGYNPDRQMRFLGQMGLSNANWQNMVIILTVISGSIMLALGLALLLRL
                     ESRRPDPVQRLYLAYCDALAKRGAARHPSEGPRDFAARVSMQFPEIKASAQKVTALYV
                     ALRYGTGEHSGKLEEMREAVRKVRQRA"
     gene            complement(3784..4977)
                     /locus_tag="VN496_14610"
     CDS             complement(3784..4977)
                     /locus_tag="VN496_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXF18237.1"
                     /translation="MTPLSTLHMVSYRIPAALTLVLVAGCGSALTAAPVIERSPNANA
                     AVKPATTAALPSPPQQQPVTPTPAPPQQQTVTPTPPPSPQQKPATQTPAQPASSPVPV
                     TPPKNDKPSNSTPPQHAADEPTTKAYREGDWRPEYHTVRKGDTLYSIALDYGQDYREL
                     AAWNNLADPALIKIDQRLRLFAPDSVGESTNPQPVALEVPLQVTVPLFSEPKARKLPY
                     SEQALAQLKLGTPKPEALAATPSPSVAVVAKAPVVRTTTPPTPGTSQGEPPVSDARMI
                     WEWPAQGKVLYGFGEGPIKKGVGIEGRTGQPVMAAAPGKVVYSGSGLRGYGKLIIVKH
                     NESYLSVYAHNSQILVKEGQTVAKGQKIAEIGNTDSDRIVLHFEIRRLGKPIDPLQYL
                     PERAS"
     gene            complement(4974..5606)
                     /locus_tag="VN496_14615"
     CDS             complement(4974..5606)
                     /locus_tag="VN496_14615"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXF18238.1"
                     /translation="MTSQRTRMRMIERLRDQGVKDEVVLKAMTEIPRHIFVDEALASR
                     AYDDLALPIGFGQTISNPFTVARMVELARGGRELVKILEIGTGCGYQAAVLSRVAREV
                     FSVERLAGLISKSRVRLRGLGLRNIKLKHGDGHAGLKEHAPFDAIVMSAAATHLPEML
                     AEQLGEGGRMVLPLGTREQRLVLVVRHADGFEQSVLDEVKFVPLIAGVSA"
     gene            complement(5673..>6277)
                     /gene="surE"
                     /locus_tag="VN496_14620"
     CDS             complement(5673..>6277)
                     /gene="surE"
                     /locus_tag="VN496_14620"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXF18239.1"
                     /translation="LIVRQSHNGFRYVNGTPTDCVHLAVTGLLDEIPDIVISGINHGA
                     NMGDDTIYSGTVAAATEGFLLGIPSIAISLASKTARNYETAARIACELFERFRDRPLC
                     PMLLNVNVPDLAYDQLEGTEVTRLGRRHKAEGVVKSTNPRGETVYWVGAAGDAQDAGS
                     GTDFHAVAHRKVSVTPLQIDLTQYNSLDRINTWLVEPALT"
BASE COUNT         1250 a         1766 c         1786 g         1475 t
ORIGIN      
        1 agttcaccag cgacttattg cccgccgata tcctcggcgt atcgatattc gaccgggaag
       61 tttcggcgtt caagttccat cctggcccga tttttacgca agtaattctt gccgacgaaa
      121 taaaccgtgc aacaccgaag gcacaaagcg cactcctgga agcaatggag gaacatcagg
      181 tcaccacgga aggccagaca cgaatcctgc ctgagccgtt cttcgtgata gctacgcaaa
      241 atccctctca tcaagtgggc acgttcccgt tgcccgaatc acagctggac cgtttcctga
      301 tgcgcattca tcttggatat ccggatgcgg cggcggaacg cgctcttttg gaaggccggg
      361 aacgccgcga tatgatttcc gagcttgggg cggcgctggc aaccggtgat ttgctggaat
      421 tgcagtacaa ggcgaggctt atttttactg cccctgcttt gttggattat gttcaggcgc
      481 tcattcgcta tacgaggcaa tcatctgatt tcgtgaacgg gctttcgccg cgcgccggtt
      541 tggcagtagt caatgccgct cgggcctggg cattcatgga cgatcgccgt gaggtcattc
      601 ctgaagatgt ccaggccgtg ttgcccagtg tagcgggtca tcggttgtcg tccatcacag
      661 ggtattcttc gttcagcagc agtcagctcg ctgagcgtct gattgctgaa gtgccgattc
      721 catagcacta cacgtgagca ctccaggcca tgacagatca atagcagtcc cgccgcattc
      781 ccgtttccgc aaactcaaaa cttcatcatc gttgcgcgac cgcgtaagcg actggatatt
      841 tccgcccaag ggtccggaaa tcagtcccat agtgctcgga cagcggcggg tttatatcct
      901 gcccaccggc gccggactca tgtttggcgt gaccatcctg gttatgctga tcggctcaat
      961 aaactataac ttgagccttg gctatgtgct ggtgttcctt ctgtctggaa acggcatggt
     1021 gtccatgctt catacatggc gcaatttggc acgcatatcg ctgcggcctg gaaaagcaga
     1081 ccctgttttt gcgggccaac gcgcagcgtt tcgtgtttac atcgagaata cttcttcttt
     1141 gcccaggacg agcctgggcg tccagcttac cgggacagaa cctgagtttc atgatgttgc
     1201 ccgcggaagc tcaaagctaa tcgaattgaa agtgtccact gcaaatcggg ggctactgta
     1261 tcccggtcgc ctacgcatat ttacgactta cccggttggc ctgttttacg catgggctat
     1321 cgtcgatctc ggcatgcatt gccttgtcta cccgcaaccg gaagctggcc gatgcatatt
     1381 gcctttggcc acgacgtcag cgggcgaagg tcaagcaatg cgcgcaggag aagaagactt
     1441 tgccggcctg cgtactttcc gtgccggcga ctccccgcgt cgaattgcct ggaaggcctt
     1501 cgcgcgcacc gaagtcttat tgtccaagca gttctcagga ggagcggccg cggaaatttg
     1561 gctggacttc cgcgacctcc ctgtcatgga aatcgaagcg aagctatcga ggctcaccag
     1621 atgggtaatc gaatcggaag ccgcgggaat acgatacggg ttgcgtctgc ccgggactga
     1681 actcgatccg gattcaggcg ttccgcatcg agaacgctgt ctgcaggcgc ttgcgctatt
     1741 caaatcgtga tagcgcgcga tctgagcccg attaatttgg ccgcgattct gggatcgctc
     1801 gcgctggtca ttggtccaca ttcccttcac cttccaggct ggatccctgc cctggccgct
     1861 gtggtgttgc tggcgcgctt ctattttggt tggcggcgga tgaagctgcc gaataaatgg
     1921 ctgctcattg cgttggcact gagcagtacg gctgccattg cgctgtcgta ccacacgctc
     1981 tatggccgtg acgtgggagt ggcgttgctt acggtcatga tagcgctcaa ggtcatggag
     2041 atgaccaagc cgcgcgacac gatggtcgtg gtgctgttgg cctatttcct tgtagtgacc
     2101 aacttcttct attcgcagtc gattccgaca gcgttctaca tgttgcttgt ggtctggatc
     2161 ataacggcca ccatgattgg attgcagcac caggctgagc agccggcagt ggtaccggtg
     2221 ctgcgccagg cagcaatcat gattctgcag ggcattccag tcatgctcac gcttttcgtg
     2281 ttgtttccgc gcgtccaggg acccctctgg ggattgcccc aaatcaatta ctcagccaag
     2341 agcggtctcg gggagagcat gacacccggc gacctaagct cactgagtct ttccgacgcg
     2401 gtcgccttcc gcgtgctctt tgaccagccg ccggctgcgc cagagcaatt ttactggcgc
     2461 gggccagtgc tctggaatta tgacgggcgt acatggcgcg ccggaaacta tgcgagcgcg
     2521 ggggcgccgt atattcaacg cctcgggccg cagctacgct atacggtcac gatggagtcg
     2581 cacgacagaa actggatgtt cgcgattgat atgccaacca gcgtgccgga tggagcttat
     2641 ctgaccacgg actatcagtt gctatcgcga cgcattgttc gcgaccgctt gcgctatgac
     2701 atgacgtcct cgccccgcta tcgagtcggc tttgacgaaa gtcccgatgc cttacgtctc
     2761 gcgcaacgct tgccttcggg agtttcgccg cgcgcgcgcg cgttggtggc gcaatggcgt
     2821 gccgaaagca tacgcgatcg tgacattgtc gttcgcgcgc tgaagatgtt tcgcgaacag
     2881 ccgttcgttt atacgctttc gccggtgccg ttggacgccg accccgtgga tcaatttctt
     2941 tttgaaacgc gcagtggctt ctgcgagcac tacgcatccg ctttcacctt cctcatgaga
     3001 gctgcgggca ttcctgcgcg ggtggtgacc ggttacctgg gaggcgaggt caatccgttg
     3061 gacggatacg tcgtggtgcg acaggctgat gcccatgcct ggtctgaggt ctggttgcct
     3121 ggcgagggct gggtcaggat agatcccact gcagccgtat cgccgttacg cgtcgagcgc
     3181 ggactggcgg aagcactacc ggcgattgac aggccattac tggcccgcgg tacctttgaa
     3241 tggctaaagc aggcaaggta tgcgtgggat gcagccgcaa acaactggaa tcaatgggtg
     3301 cttggctaca atccggatcg ccagatgcgt tttctcggac agatggggct tagcaacgcg
     3361 aactggcaaa acatggtgat cattctgacc gtgatcagtg gctccatcat gcttgcgttg
     3421 ggtctggcgc tgctattgcg actcgaaagc cgacgtccgg atcccgtgca gcgcctctat
     3481 ttggcctatt gcgatgcgct ggcaaagcgc ggcgccgccc gccatccaag cgaagggccc
     3541 cgggattttg ccgccagagt gtcgatgcaa tttcccgaaa tcaaggcatc agcgcaaaag
     3601 gtaaccgctc tgtacgttgc gcttcgctac ggaacaggcg agcacagcgg caaacttgaa
     3661 gagatgcgcg aagcggtgag aaaggtcagg cagcgcgcct gatcctggcg tcaatcaagg
     3721 ctggcttccg gcgccgtacc ccataagaca tccgccactc cttgtgaaca gagcaacttt
     3781 gtttcacgag gcgcgctcgg gaagatattg aagcggatca attggcttgc caagacggcg
     3841 gatctcgaaa tgcaagacaa tacgatcact gtcggtattg ccgatttcag caatcttttg
     3901 ccccttggcg accgtctgtc cttccttgac cagtatctgg ctgttatggg catacacgct
     3961 gagataagac tcattgtgtt tgacgatgat gagcttgccg taaccacgta gtccacttcc
     4021 gctatatacc accttacccg gcgctgctgc catgacgggc tggcctgttc gaccttctat
     4081 tccgactcct tttttgattg gtccctcgcc aaatccgtac aatactttcc cttgcgctgg
     4141 ccactcccaa atcatcctgg cgtcgctgac cgggggctcg ccttgcgaag ttccgggggt
     4201 aggcggagtt gttgtgcgga caacgggtgc tttggcaacc accgctacgc tgggagatgg
     4261 agtagctgca agcgcctccg gctttggcgt gccaagcttt aattgcgcca gcgcctgctc
     4321 cgagtaaggc agctttcgtg ctttgggttc cgaaaaaagc ggtaccgtca cttgcagtgg
     4381 tacctcgagt gcaaccggct gagggttagt tgattctccg acgctgtctg gagcaaacag
     4441 cctcagacgt tgatcgattt ttatcagcgc cggatctgcc agattgttcc aggccgccag
     4501 ttctcgataa tcctgaccat agtcgagcgc tatgctatac agcgtatctc ccttgcggac
     4561 ggtgtgatat tcgggtcgcc agtcaccttc acgataggct ttggttgtcg gctcgtccgc
     4621 cgcgtgctgt ggtggtgttg agttggatgg cttgtcattt tttggcggag tcaccggtac
     4681 cggtgaactc gccggctgtg caggcgtctg cgtcgcaggt ttctgctgag gagatggtgg
     4741 cggcgtaggc gtaacagttt gctgttgcgg tggtgctggc gtaggagtga caggttgctg
     4801 ttgcggtggt gaaggcaatg cagccgtggt tgccggtttg acagcagcat tggcattcgg
     4861 cgaacgctct attaccggcg cggcagtcag ggccgagccg cagccagcga caagtaccag
     4921 tgtcagcgct gctggaattc gataactaac catgtgtagt gttgaaagcg gcgtcacgcc
     4981 gatactccgg cgatcaacgg cacaaatttc acttcatcca aaactgattg ttcaaaaccg
     5041 tccgcgtggc gtactacaag taccaggcgc tgttctcgcg tacctaacgg caaaaccatc
     5101 cgccctcctt cccccagctg ttctgcgagc atctccggca aatgagtcgc cgcggcagac
     5161 attactattg cgtcaaacgg agcatgctct ttgagtcccg catgcccgtc accatgtttc
     5221 agttttatat tgcgaaggcc gagtccacgc aaacgtactc tggacttcga aataagcccg
     5281 gcgagccgtt ccacagaaaa cacttcgcgc gccacacgtg acaataccgc tgcttggtaa
     5341 ccgcatccag ttccgatttc caggatcttg accagttcgc gaccaccacg tgcgagttca
     5401 accatgcgcg ccacggtaaa aggattggaa atggtctggc cgaaaccgat cggcagtgcc
     5461 aggtcgtcat aagcgcggct tgcaagcgcc tcatcaacga aaatatggcg cggtatttca
     5521 gtcatggctt tcaaaacgac ttcgtccttc acgccctggt cgcgcaagcg ttcaatcatc
     5581 cgcatgcgcg tgcgctgtga agtcatgcct atgccggcat tcctgttatt catctgccag
     5641 gagcacttcc caccctgcgt actgatgcac cctcatgtga gtgcgggttc cacgagccag
     5701 gtgttaattc gatcgaggct gttgtactgg gtgagatcaa tttgtagcgg cgttaccgag
     5761 accttgcgat gggcaactgc atgaaaatcc gtacctgaac ccgcatcctg cgcatcacca
     5821 gcagcgccga cccaatagac tgtttcgccg cgcggattcg tcgactttac gactccctcg
     5881 gctttatgcc gccgaccaag tctcgtcact tcggtgcctt caagttgatc ataggcaaga
     5941 tcaggcacgt tgacattgag cagcattgga cagaggggtc tatcgcgaaa gcgctcgaat
     6001 aattcgcagg caatgcgagc tgcggtctca tagttgcgtg cggttttgct ggcaagggag
     6061 atcgcaatgg acgggatgcc caatagaaat ccttccgttg cggccgccac ggtacccgaa
     6121 taaatggtgt cgtcgcccat atttgcaccg tgattgatgc ccgatatcac gatatccggg
     6181 atctcgtcga gaagtccagt caccgccaaa tgcacgcagt ccgtcggcgt gccattgacg
     6241 tagcggaaac cattgtggga ctgccttaca attaaag
//
LOCUS       DATMZQ010000461         3345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4381924, whole genome shotgun sequence.
ACCESSION   DATMZQ010000461 DATMZQ010000000
VERSION     DATMZQ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4381924"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VN496_14625"
     CDS             <1..486
                     /locus_tag="VN496_14625"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXF18240.1"
                     /translation="IYEGTTGIQANDLVGRKIAYEKGSTINILLAEMRAVDAKLAKVG
                     HPGLVVIRRALGEGVAALSEATDWLLSTFPNNMKAASAGAVPFLKLFGTVAGGWQMAR
                     AALIAKSRLDENAEDYDFYRAKIGTARFYAEHILPLAAAYKHEIVHGSTSVLALEEAQ
                     F"
     gene            659..3151
                     /locus_tag="VN496_14630"
     CDS             659..3151
                     /locus_tag="VN496_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXF18241.1"
                     /translation="MNAILWFIAAASVLWILAFLRAGTATWAITIGLYLVALTQWSGA
                     GGSTKAALWTAFLIVAALLMVRQLRRALISDPLLNWFRKALPQVSQTEQEALDAGTVW
                     WDGELFSGQPDWNKLFGTPKPRLTPDEQAFLDGPVEEVCRLCNEWEISHELNDLPPHV
                     WQFVKDRGFMGMIIPRQYGGLGFSAYAHSQVVQKLATRSATACVSVMVPNSLGPAELL
                     LHYGTPEQKNYYLPRLAQGLETPCFALTGPEAGSDAGSIPDFGIVCKGLHDGREVLGI
                     RVTWEKRYITLGPVATLLGLAFKLYDPEKLLGKELDIGITLALIPTKHPGVNIGRRHL
                     PMNASFMNGPNWGRDVFIPLDWVIGGREFAGQGWRMLMECLAAGRSISLPSSSAGAAK
                     LALLTTSAYSRVRSQFKIPIGKFEGIEEVLARMAGNTYAIDAVRTMTAGAVDLGEKPS
                     VISAIVKYHCTERSRQVINDAMDVHGGKGICLGPNNYLGGAYQQIPIGITGEGANILT
                     RSMMIFGQGAIRCHPYLLKEIAATRNPDIEQAGSQFDAAFWQHVRFVACNAARSLWHG
                     ITAARLINVPGALELRSYIRQISRLCASFAFASDITMLLLGGDLKRREKLSARLGDIL
                     SQLYIVSTVLKRYEDDGRPTADLPLIKWAIEDCLTRIQDAFYGVFLNFPSRILGFILR
                     VSIFPWGKSFRAPRDYLGHQIARLLLEISPTRERLTSNVFILRHEEDPVGRLELAMQL
                     AAAGDAAEAKVRSAVKAGVIDGMTEPERIASAIEKRIITTAEAVQFQRFSALRRACIM
                     VDDFPHDIGRSQAARESASITALAAPSQKTAA"
BASE COUNT          740 a          894 c          973 g          738 t
ORIGIN      
        1 atctacgaag gcaccacagg aattcaagcg aacgacttgg tcggccggaa gatcgcttac
       61 gaaaagggtt caaccataaa tatcctgttg gctgagatgc gggctgtcga tgcaaaactc
      121 gccaaagttg gccaccctgg cctggtggtg attcgccgcg cgctcgggga aggcgtggcg
      181 gcgttatctg aagcgaccga ctggctactt tctacttttc cgaacaacat gaaagcggca
      241 tccgccggcg ccgttccctt tctgaaactt tttggcaccg ttgcgggggg gtggcaaatg
      301 gcgcgtgctg ctttgatagc caagagcagg ctcgatgaaa atgcagagga ctacgatttc
      361 tatcgggcca aaatcggaac cgcgcgattt tatgccgaac atatcctgcc gcttgcggcc
      421 gcgtacaagc atgagatcgt gcatggttca actagcgtac tcgcactgga agaggctcaa
      481 ttttgaggga agcatcaaag cggcgtgatg acgatgctaa cgtcgctgga aaagaaagaa
      541 ttcttccacg gagcgaccgg caattcgcag tttcgttggt ccgccagtcc agaaatgcat
      601 gcaacgttcg gcaacgtcgc atgttaaagc caaccgattt ggaaatagga gaaacagcat
      661 gaacgctatt ctttggttca tcgccgctgc cagcgtgtta tggatcctgg cgtttctccg
      721 tgccggtact gcgacgtggg ctatcaccat cggtctctat ctcgttgcct tgacgcaatg
      781 gtctggcgca ggcggctcga cgaaggcggc attatggacc gcattcctca tcgtggccgc
      841 acttctgatg gttcgtcaac tgcggcgcgc ccttattagt gatccgcttc ttaactggtt
      901 tcgaaaggcc cttccgcagg tatcgcaaac cgaacaggaa gcgcttgatg cggggaccgt
      961 atggtgggat ggcgaattat tcagcggcca accggactgg aacaaattat tcggaacgcc
     1021 aaaaccccgc ctcactcccg acgagcaggc ctttcttgac gggccggtgg aggaggtatg
     1081 ccgcctttgt aacgaatggg aaatttctca cgagctcaat gatctgccgc cgcatgtatg
     1141 gcaatttgtc aaggatcgcg gcttcatggg aatgatcatt ccaaggcagt acggcggcct
     1201 ggggttctct gcatatgcgc actcccaagt tgtacagaag ctcgcgacgc gcagcgcgac
     1261 cgcttgtgta tcggtcatgg tcccgaactc gctcgggcca gcggaacttt tgctgcatta
     1321 cgggacgcct gaacaaaaaa attactatct cccacgtctg gcgcagggcc tggagacccc
     1381 ttgctttgca ttgactggtc cggaagccgg ctcggatgct ggttcaattc ccgatttcgg
     1441 catcgtatgc aagggattgc acgatgggcg cgaggtgttg ggcatccgcg tgacgtggga
     1501 aaaacgatac ataacgttgg gcccggtcgc aacgctgctc ggtcttgcat tcaaattgta
     1561 cgatcccgaa aaattgctgg ggaaggaact ggacatcggc attacgctcg cattgatacc
     1621 gacgaagcac cccggtgtga acatcggccg ccgacacttg ccgatgaatg cttcattcat
     1681 gaatggtcct aactggggca gggatgtttt cattccgctt gactgggtaa taggcgggcg
     1741 cgaattcgcg ggtcagggct ggcgcatgtt gatggagtgc cttgcggccg gacgctcaat
     1801 ttcccttcct tcatcgagcg ccggtgcggc gaaactggca ttacttacca ccagcgcgta
     1861 cagcagagtg cgcagccaat tcaagattcc tattggaaag ttcgaaggta ttgaggaagt
     1921 cctggcgagg atggcgggca atacctatgc gatcgacgcc gttcgcacca tgacggcagg
     1981 tgcggtggac cttggcgaaa agccctctgt catttctgca atcgtcaaat accactgcac
     2041 cgagcgcagc cgccaggtca ttaacgacgc gatggatgtg cacggcggca aaggcatttg
     2101 tcttggaccg aacaattacc ttggcggcgc gtatcagcaa ataccgatcg gaattaccgg
     2161 cgaaggtgca aacatcctca cccgtagcat gatgatattc ggccaaggtg ccatcaggtg
     2221 ccatccgtac ttgctaaagg aaatcgcagc gacgcgcaat ccggatatcg agcaggctgg
     2281 aagccaattt gacgctgctt tctggcagca tgtgcgcttc gttgcatgta acgcggcccg
     2341 atcgctgtgg catggaatta ccgctgcccg cctgatcaat gttccgggcg ctctggagtt
     2401 gcgcagctac atacggcaaa tttcaaggct atgcgcgagc tttgcttttg cctccgacat
     2461 aacgatgttg ctgctgggag gcgacctgaa gcggcgtgag aaattatcgg ctcgtctcgg
     2521 cgatatcctg agccaacttt atatcgtttc tacggtgctg aaacgatatg aagatgacgg
     2581 gcggccaacg gccgacttgc cattgatcaa atgggcgatt gaggactgct tgactcgtat
     2641 tcaggatgcg ttttatggcg tctttctgaa ttttccgagt cggatcttgg gattcatact
     2701 gcgcgtgtcg attttcccgt ggggaaaatc attcagggca cctcgcgact atctgggaca
     2761 ccaaatcgca agattactgc tggaaatctc accgacccgc gaacgcctga cctccaacgt
     2821 tttcatattg cggcatgaag aggatccggt gggccgtctt gaactggcca tgcaattggc
     2881 tgccgccggt gacgcggcgg aagccaaagt gcgaagtgcc gtcaaggcgg gcgtaatcga
     2941 cgggatgacc gagccagagc gcattgcttc ggccatcgaa aagcgaatca ttaccaccgc
     3001 agaggctgtc cagttccagc gcttcagcgc gctgcgccgt gcctgcatta tggtggacga
     3061 tttccctcac gatatcggcc ggtcacaggc ggcacgtgag tccgccagca taacggcgct
     3121 ggcagcccca tcacaaaaga ctgccgcatg agcggctgca gtaaatctct tactggcgga
     3181 gtgacatagc aacgattgtc ctcaaccggc cgcacgtgtt gaacgctatg gacgcagaga
     3241 tgatgcgtga aatgcgccgc atcacagaag cggttgaacg ggatgcctct gttcgcgcag
     3301 cgatcctcgc gagcgtacac ggggcagttg cagggaccgg cttca
//
LOCUS       DATMZQ010000462        11212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3693959, whole genome shotgun sequence.
ACCESSION   DATMZQ010000462 DATMZQ010000000
VERSION     DATMZQ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693959"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1203
                     /locus_tag="VN496_14635"
     CDS             400..1203
                     /locus_tag="VN496_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXF18242.1"
                     /translation="MQHITSRHNALYKQARKLTGSARERHKQGRIVMDGTRLIRAYAD
                     QFGLNDSMLFVSQHGATHPDIQQILKTRSAQQTYSLSDALFGEIAQVVTPEGIIAIAD
                     TPQVPPKTSDDFRVLLEGVQDPGNLGGLLRTAAAAGATSADLTNGCADAWSPRSLRGG
                     MGAQFVLCMRERTTLATSLADFHGTVIGTSARAEKAIYDIDLSGPVMMIFGNEGKGLP
                     DEVLSMAHQLARVPMAGPIESLNVAAAAAICCFERLRQTRVSAGKSFRR"
     gene            complement(1941..2717)
                     /locus_tag="VN496_14640"
     CDS             complement(1941..2717)
                     /locus_tag="VN496_14640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXF18243.1"
                     /translation="MFVDSHCHINFPDLAADIAGVLGAMSANKVSHALCVSVNLESFP
                     EVLGLAERFPNIFASVGVHPDDRSGEEPDEARLTSLARHPKVVAIGETGLDYYRVEGD
                     TGWQQERFCTHIRAARQVGKPLIVHTREAAGDTLRIMREETAAEVGGVMHCFTETAEV
                     AYQAMDMNFMISFSGIVTFRNAKALKEVARDVPLDYLLIETDSPYLAPIPFRGKTNQP
                     AYVRFVAEEIGRLKGISTEAVAEATTRNFFRLFRSATKVS"
     gene            complement(2754..3758)
                     /gene="holB"
                     /locus_tag="VN496_14645"
     CDS             complement(2754..3758)
                     /gene="holB"
                     /locus_tag="VN496_14645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXF18244.1"
                     /translation="MENLTYPWVAPLLARLPRSATALPRALLLTGRRGLGKRATALFL
                     AQGLLCEAHRDKLEACGTCPSCLLYRVGNHPDLRMLEIGFEDETQAFADDELQPAKKQ
                     SRHISVDKVRTLLDFVTITAHRGGAKVVCIMPVESMLPAAANALLKILEEPPGNTFFL
                     LVSHQPERLLATIRSRCFQVAFGLPDRQPALDWLREQGIEEAELALAQGGYAPLAAID
                     RAGDEQFWLQRKVLLDALGSGSFDALRVAEYAQDIDGALAATLLSQWVYDIAAVQSGA
                     SVRYHLDYTSTLQKLAGSLSAYELMKWYDAIVRFGRIAEHPLNKRLAMEDLLAGFPNQ
                     "
     gene            complement(3746..4378)
                     /gene="tmk"
                     /locus_tag="VN496_14650"
     CDS             complement(3746..4378)
                     /gene="tmk"
                     /locus_tag="VN496_14650"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXF18245.1"
                     /translation="MKRGLFITLEGIDGAGKSTHMAWSANYLQECGLSVISTREPGGT
                     RLGEELREVLLNAKEPIHPETEALLMFAARRQHLEEVIMPALRRGDCVLCDRFTDASF
                     AYQGGGRGVDHAKLAALEAWVHPGLEPDLTVLFDVSAEVSHERVARIKSPDRFEREDA
                     QFFTRVRAAYLERLKESPDRFLRVDGAGAIAQIQAELAKQFTLTLSKWKT"
     gene            complement(4375..5367)
                     /gene="mltG"
                     /locus_tag="VN496_14655"
     CDS             complement(4375..5367)
                     /gene="mltG"
                     /locus_tag="VN496_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXF18246.1"
                     /translation="MRLLRAGLLACVLLAGCAAAWLTYFAFHAIDVPSNVRTFNVDHG
                     RSLRSISDQLAQAGLVSDQWSFQALARLMGAAGEIKAGSYEVGPRIAPYQLLEKIVKG
                     EFAQAEVKFIEGWTFAQLRSALDTHPAIKHDSAGLSDAQILQRLAIDKSSPEGLFFPD
                     TYYFAVASSDLALLKQAYLRMQSKVQTLWGQRAAGLPLRNEYEALIFASIVEKETGRS
                     DERQLVSAVFVNRLRRGMRLQTDPTIIYGLGAAFDGNLHRRDLVNDTKYNTYTRYGLP
                     PTPIAMPGEASIWAALNPAKSSVLYFVGRGDGSHEFSSNLADHNRAVNKFQLHR"
     gene            complement(5496..6740)
                     /gene="fabF"
                     /locus_tag="VN496_14660"
     CDS             complement(5496..6740)
                     /gene="fabF"
                     /locus_tag="VN496_14660"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXF18247.1"
                     /translation="MSRRRVVVTGLGTVCPVGNSVKEAWSNIQAGKSGVTRITRFDAS
                     PFASQIAGEVKGFDIGTVLTPKEARRVDLFIHYGIAAAAEAIKDAGLEAHPKDAERVG
                     INVGSGIGGLSLIEETHKAMLEGGPRKISPFFIPGAIINMISGNLSIMYGFKGPCLAM
                     VTACTTANHCIGDSGRLIEYGDADVMVAGGSESTVCALGIGGFSAARALSTRNDDPAT
                     ASRPWDTDRDGFVLGEGAGVVVLEELEHAKARGARIYCELVGYGVSADAHHITAPCED
                     GAGAARGMMNAMRNAGVNPTEVDYINAHGTSTPLGDLAETIAVKRCFGDHAKKLAISS
                     TKSSTGHLLGAAGGVEAVFTIMAMNDKVAPPTINMFNQDPECDLDYVPNTARQMKIKV
                     SLSNSFGFGGTNGTLVLRTFDQ"
     gene            complement(6775..7011)
                     /gene="acpP"
                     /locus_tag="VN496_14665"
     CDS             complement(6775..7011)
                     /gene="acpP"
                     /locus_tag="VN496_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXF18248.1"
                     /translation="MESVEQRVKKIVAEQLGVNEADVKIESTFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKITSVQQAIDYIKAHLN"
     gene            complement(7125..7865)
                     /gene="fabG"
                     /locus_tag="VN496_14670"
     CDS             complement(7125..7865)
                     /gene="fabG"
                     /locus_tag="VN496_14670"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXF18249.1"
                     /translation="MLDGKVVLITGASRGIGQAIALGLAEAGATIAGTATSESGAAGI
                     STYLRDAGFKGNGYKLNVNNGAETQAVFAAIESQHGSVAILVNNAGITRDNLLLRMKD
                     TEWDEIMETNLKSVFRLSKLVIRSMMKAHFGRIINITSVVGSMGNAGQVNYAAAKAGV
                     VGFSKSLAKEVGSRNITVNCIAPGFIDTDMTRALPEEQKKALLGHIPLGRFGQAGEIA
                     AAVAFLCSDGAAYVTGSTLHVNGGMYMD"
     gene            complement(7875..8807)
                     /gene="fabD"
                     /locus_tag="VN496_14675"
     CDS             complement(7875..8807)
                     /gene="fabD"
                     /locus_tag="VN496_14675"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016723251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXF18250.1"
                     /translation="MKAAFVFPGQGSQSVGMMRSFADLAMVRETFSEASDVVGDDLWK
                     LVQEGPADALSLTINTQPVMLAADTALYRAWKSHYGTLPIIAAGHSLGEYGALVAGDA
                     MDFAEAVSLVRYRAQAMQEAVPENVGAMAAILGLEDAEVEDACAEASEPGQTVEAANF
                     NSPAQVVIAGHKGAVERAMDIAKGKGAKRAIMLPMSVTSHCSLMRPAAERMRERLKSV
                     AIRMSTVPVLHNATVRSASSVQDLRDALVRQLYQPVRWVDTIRAMLSAGATHIVECGP
                     GKVLAGLNKRIAPETPVISFADESAFQAALIALK"
     gene            complement(8810..9766)
                     /locus_tag="VN496_14680"
     CDS             complement(8810..9766)
                     /locus_tag="VN496_14680"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HXF18251.1"
                     /translation="MFSRIVGTGSYLPDRIVTNDDLARTLDTSDEWIRTRTGICSRHV
                     AADGQLASDLALPAAQRALKMAGLDAAEIDLIIVATTTPDMIFPSTACILQAKLEAAG
                     CPAFDVQAVCSGFVYALSIADLFVRAGKAKKVLIVGTEVYSRILDWSDRGTCVLFGDG
                     AGAVIVVASDKPGIIESRLHADGRHKDMLCVPGSVNGGKVWGSPFVHMDGGAVFKFAV
                     RVFEEGANEVLQAAGLTISDVDWFIPHQANIRIMEATARKLGLPKEKVIVTVHHHGNT
                     SAASIPLALDEAVKDGRIVDGQMLLLEGVGGGFTWGAALLRW"
     gene            complement(9790..10779)
                     /gene="plsX"
                     /locus_tag="VN496_14685"
                     /pseudo
     CDS             complement(9790..10779)
                     /gene="plsX"
                     /locus_tag="VN496_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
     gene            complement(10920..11099)
                     /gene="rpmF"
                     /locus_tag="VN496_14690"
     CDS             complement(10920..11099)
                     /gene="rpmF"
                     /locus_tag="VN496_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXF18252.1"
                     /translation="MAVQQNKKSASKRGMHRSHDFLTNPTLAVEPTTGETHLRHHISP
                     NGFYRGKKVVKTKGE"
BASE COUNT         2499 a         3258 c         2906 g         2549 t
ORIGIN      
        1 ttacttaagt ctttggattt gcaaaatagg gcaggattgc catgcttcgc gcgacgctcg
       61 atcttttaac tagatcgctt tgtcacggaa ggagtaagga tagctaccgc aagtacattc
      121 tgcaacgagc tttacaaaaa atcgtcacac tgcgaatggt gcacgctgac ggaataccaa
      181 gaataatcta aaagaaacaa attggaatct cggacagtgc tatatgtgct tgaggtttgg
      241 gttgttccga aacgcacggt agttcatgag caaaagtgcc agccatcgtc ttttaactcg
      301 tatttagccg ttatcgcttg gtcttgcctc acccgtcgct tgaacgcagt tgcgcaattg
      361 ttcagcagaa tgagattcga tttcgccgcg agagtataga tgcagcacat aacctcacga
      421 cacaacgcgc tctacaagca ggcacgcaag ctcacaggct cagcacgcga gcgacacaaa
      481 cagggtcgga ttgttatgga cggcacgcgc ttgatcaggg cgtatgccga tcaattcggc
      541 ttgaacgatt ccatgctgtt cgtaagccag cacggtgcga cgcatccgga catccaacag
      601 attctgaaga cgcgttctgc tcagcagacc tactcgctct ccgatgctct tttcggagaa
      661 atcgcgcaag ttgtcacacc agaaggcatc atcgccatcg cggacacgcc gcaagtcccg
      721 cccaaaacat ccgacgattt tcgtgtactg ctggaaggcg tacaggaccc tggaaacctg
      781 ggcggtctgc tcaggactgc ggcggccgca ggtgctactt ctgctgacct gaccaatggg
      841 tgtgccgatg cgtggtcgcc aaggtcgcta agaggcggca tgggtgcgca gttcgtgctc
      901 tgcatgaggg aacgtacgac gcttgccact tcactggcag attttcacgg caccgtgatc
      961 ggcaccagcg cgcgcgcgga aaaagcaatt tatgacattg atctgtccgg cccagtaatg
     1021 atgattttcg gcaacgaagg caaaggatta ccggatgaag tactgtctat ggctcatcaa
     1081 ctcgcgcgtg tgccgatggc cggtcctatc gagtctctaa acgtggccgc tgccgcagcg
     1141 atctgttgct ttgaaaggct acgccagacg cgggtgtcag cgggcaagtc gttcaggcgg
     1201 tgaggggatg ccctagcgct caactgcgca ggcctttatc gttgtactcg gacggccttc
     1261 gaaggtcccg tagccgtcaa attatttttt ccccgcaatg tagatcagtg ggctcctgaa
     1321 ccggaatacc gtcccaggca gcgataaatc cctggagttg ttccgacgtc cccggcgaaa
     1381 agtaatacca atcgccgcca tcggggaagc tcttccggac aacatggcat tttagtaccc
     1441 ccccccaagc tgcgaaagct ctttgaaaag cagattttgg agcccgacgc cgcctgcccg
     1501 cttgggcaag tggagtttga tgcagtgcca tcccatgttt gaaggtcttt gttatcgtaa
     1561 tcgggtggga gattgtactt ggaggggcaa actatcgcat gtgtgtcaac gcactgaaac
     1621 cacgcgtaac tttgttgttg agggtgctgg ataactggaa gtcccaaccg tttttgcacg
     1681 cttgattttt cgcaacgtcc cacgatttta aagtcaacaa ggccgaccca agaattccgt
     1741 aagggtgtcg cgcgaacttt gcgcctacag gcctccacgg gcctttttgt attctgctat
     1801 agcgcttgaa gaattaccga gatgatgctg aaagggcaga agttagggcc aattcgaatg
     1861 gccgaaacag acgctatctt aaagtatttt attaatttaa cataatatac attatgcgca
     1921 ttttaaacct acttggttca tcaagaaacc ttcgttgcgc tccgaaacaa tctgaagaaa
     1981 ttgcgtgtag ttgcttcagc tacggcttca gtcgagattc ccttgagccg gccaatttcc
     2041 tcggcgacga agcgtacata agccggttga ttggtcttgc cccgaaatgg aatgggcgcc
     2101 aggtacggtg aatcagtttc gatcaggaga taatccaaag gcacgtcgcg cgcgacttcc
     2161 ttgagtgcct tcgcattcct gaacgtcacg attccggaaa atgaaatcat gaagttcata
     2221 tccatggctt gataagcgac ctcggcggtt tccgtgaagc aatgcataac gccaccgact
     2281 tccgcagccg tttcctcgcg catgatgcgc agcgtgtcac ccgcggcctc ccgcgtatgc
     2341 acgatcaatg gtttcccgac ttgcctggca gcgcgaatgt gcgtgcaaaa gcgctcttgc
     2401 tgccaaccgg tatcgccttc aacgcggtaa taatccaggc ccgtttcacc gatggcgacg
     2461 accttggggt gcctggccaa gcttgttagc ctggcctcat caggctcttc tcccgaccgg
     2521 tcgtcgggat gtacaccaac cgatgcaaat atgttgggaa agcgttcggc gaggccgagc
     2581 acctcaggaa acgattcaag attcactgaa acacataggg cgtgactaac cttgttcgca
     2641 gacatcgcgc caagtacgcc ggcgatatcg gcagccagat ccgggaaatt tatgtggcag
     2701 tgggaatcga cgaacaattc aatagcccca gggacagcaa tgcagtcgcc agtttattga
     2761 tttgggaaac cagcgagaag atcttccatg gccagccgct tgttgagggg atgctctgct
     2821 atgcggccaa atcgaacgat ggcgtcatac cacttcatga gttcatatgc agagaggcta
     2881 cccgctagtt tctgcagggt tgatgtgtaa tcgaggtgat aacgcacgct tgctcccgat
     2941 tgaacggccg cgatgtcata aacccattga gacagcaacg tcgccgcgag cgcaccatcg
     3001 atgtcctgcg catactccgc aacgcgcaat gcatcgaacg agccactgcc aagcgcgtcc
     3061 agcaaaacct tccgttgcag ccaaaattgc tcatccccgg cccgatcaat tgcggccagc
     3121 ggagcatacc ctccctgggc cagcgccagt tccgcctcct ctatcccctg ctctctcaac
     3181 caatccaacg ccggctgccg atcgggaagg ccaaacgcga cctggaaaca gcggcttcgt
     3241 attgtggcca gcaagcgctc gggctgatgg ctcaccaaca agaaaaacgt gtttcctggc
     3301 ggttcttcga gtattttgag taaggcgttg gcggcagctg gcaacatgga ttcaaccggc
     3361 ataatgcata caacctttgc gccaccgcga tgcgccgtga tggtcacaaa atccagcagg
     3421 gttcgaactt tgtccaccga gatgtgacgg ctctgtttct ttgccggttg cagttcgtca
     3481 tcagcgaaag cctgagtttc atcttcgaat ccgatttcga gcatgcgtaa atcaggatgg
     3541 ttcccgaccc gataaagaag gcacgacggg cacgtcccgc acgcttccaa tttgtcccta
     3601 tgcgcttcgc ataacaaacc ctgcgcaagg aaaagcgcgg tagcacgctt gcccaaccct
     3661 cgtcttcctg tcaaaagcaa cgcccggggc agcgctgtcg cgctgcgcgg taacctcgcg
     3721 agcaacggcg cgacccaggg ataagtcagg ttttccattt cgacaacgtc agcgtgaatt
     3781 gcttcgccag ttctgcctga atttgcgcaa tggcaccagc gccatcgacg cgcaagaatc
     3841 tgtcgggaga ctctttcaat cgttcaaggt acgccgcgcg aactcgggta aaaaattgcg
     3901 catcttcgcg ttcaaaccgg tcgggagatt tgatacgggc gacccgctcg tgacttactt
     3961 ccgcactgac atcgaacagc actgtaagat ccggttccag accgggatgc acccaggcct
     4021 ccagcgcagc tagtttggca tggtcgacgc ctcgtccgcc gccctgatag gcaaagctgg
     4081 catccgtgaa gcgatcacag agcacacaat ctccccgccg caaagccggc atgattacct
     4141 cttccagatg ttgccgccta gcggcaaaca tcagcagggc ctccgtttca ggatgaatcg
     4201 gctccttcgc gtttaacaac acctcacgca gttcttcgcc cagcctcgta ccgccgggtt
     4261 ctcgcgtgga aatcacggaa agcccacatt cctgcagata attggcgctc catgccatgt
     4321 gggtactttt acccgcgcca tcgataccct cgagggtaat gaacaatccg cgcttcaacg
     4381 gtgaagctgg aacttgttca cggcgcggtt gtggtccgcg agattggatg agaactcatg
     4441 gctaccgtcc ccacgcccta cgaaatacag taccgagctt ttcgcggggt tcagtgctgc
     4501 ccagatggat gcctctcccg gcatcgcaat gggcgttggg ggaaggccat atcgcgtgta
     4561 ggtgttgtat ttcgtgtcat ttaccagatc acggcgatgc aaattaccat cgaacgcagc
     4621 acccaagcca taaatgatgg ttggatcggt ctgcagccgc atgccgcgtc gtagccggtt
     4681 gacgaatact gccgagacca gttgcctttc gtcgctgcgg ccagtctctt tttcaacgat
     4741 ggatgcaaat atcaacgctt catattcatt tcttaagggc aaccctgcgg cgcgctgtcc
     4801 ccacagagtt tgcactttcg attgcatgcg gagataagcc tgcttcagca gtgcgaggtc
     4861 gctggatgcc acggcgaaat agtaggtatc cgggaaaaac aatccttcgg gggacgactt
     4921 atctatggcg agccgctgca atatctgtgc atctgacagg ccggcgctgt catgttttat
     4981 agccgggtgt gtatccagag cgcttcgcag ctgagcaaac gtccagcctt cgatgaattt
     5041 gacttcggct tgcgcaaact cgcctttgac gattttttcc aatagctgat agggtgcaat
     5101 gcgtggccct acttcgtaac ttcccgcttt gatttctcct gcggcaccca tgaggcgggc
     5161 gagggcctgg aaactccact ggtccgaaac cagtcctgct tgtgcaagct ggtctgaaat
     5221 actgcggaga ctcctcccat ggtcgacatt gaaggtccgc acatttgacg gaacatcaat
     5281 cgcatgaaaa gcgaaatacg ttagccaggc agccgcgcaa cccgctagca gaacgcacgc
     5341 cagcaggcct gcacgcaaga gccgcatgaa tgtccatctg ggtgccggca ggaagagatt
     5401 tcgatttgcc atgctaacaa cgtcaccacg aaaccatgat tgttactcta acgcgccaga
     5461 ttggccgaat ccccggtggg aattgcggca tccgtctatt ggtcgaatgt ccgcagcacc
     5521 agcgtgccgt tggtaccacc aaatccgaag gaattagaca aggaaacttt gattttcatc
     5581 tgcctggcgg tattcggcac gtaatcaaga tcacattccg gatcctgatt gaacatgttg
     5641 atcgttggcg gagccacttt gtcattcatg gccatgatcg taaatacggc ttcgacgccg
     5701 ccagctgcac ccagcaagtg gccagtgctg gacttggtgg aactaatggc cagttttttt
     5761 gcgtgatctc cgaaacagcg cttcaccgcg attgtttccg cgagatcacc cagcggcgtg
     5821 gaggtgccgt gcgcattaat atagtccact tccgttggat taaccccggc attgcgcatt
     5881 gcattcatca tgccgcgcgc agccccggca ccgtcttcac agggagccgt gatgtgatgc
     5941 gcgtccgcac tgacgccata gccgacgagt tcgcaataaa ttcgtgcccc gcgcgctttc
     6001 gcgtgttcca gttcctcaag aacaaccaca ccggcaccct cgccaagaac gaatccatcc
     6061 cggtcggtat cccacggccg gctggccgta gcaggatcgt cattgcgcgt gctgagtgcc
     6121 cgcgccgctg aaaacccgcc tatgccaagc gcgcagactg tcgattcgga accgcccgcg
     6181 accatgacat cagcatcgcc atactcgatg agacgaccgg aatccccgat gcaatgatta
     6241 gccgtggtgc aagctgtaac catcgcgaga cacgggccct tgaagccata catgatcgac
     6301 agattcccgg aaatcatgtt gataatggcg ccaggaatga aaaacggaga aatctttcgc
     6361 ggcccgccct ccagcatggc tttgtgtgtt tcttcgatca gggataaccc cccgataccg
     6421 gacccaacgt tgatgccgac tcgttcagcg tctttcggat gagcttccag tccggcatct
     6481 ttgatggctt cagccgcggc ggcgattccg taatggatga acaggtcgac ccggcgcgcc
     6541 tccttcggcg tcaatactgt gccgatgtcg aatcctttca cctcgcctgc aatttgggat
     6601 gcaaacggtg atgcatcaaa tcgggtaatc cgtgtcactc cggacttacc tgcctgaatg
     6661 ttgctccacg cttccttgac gctgtttccg accgggcaga cagtacccaa accggttacg
     6721 acaactcggc gtctagacaa cggaactcca ggttggaaag aatatcggga acgttcagtt
     6781 taggtgtgcc ttgatgtagt cgatcgcctg ctggacagac gtgatctttt ccgcttcttc
     6841 atcggggatc tcgcactcga actcctcttc aagtgccatg actaactcta cggtgtcgag
     6901 tgaatcggcc ccgaggtcat caacaaaagt tgattctatc ttcacatcag cctcattcac
     6961 gcctagctgc tcggcaacga ttttcttgac gcgctgttct acggattcca ttaatgtccc
     7021 ttcccttttg cttgaagatt ctattgcgtg acttccgtac cttgttaagg cgcctgcagc
     7081 aaaaacacgc ggcacattct accaaagttg catgccgtac ggcatcaatc catatacatg
     7141 ccaccattga catgcagggt gcttcccgtc acatacgcag ctccatcgga gcagaggaag
     7201 gctaccgcag cagcaatctc cccggcctgt ccaaagcggc ctagcgggat atgccccagc
     7261 aaagctttct tttgctcttc aggcaatgca cgcgtcatgt cggtatctat gaatcctggg
     7321 gcgatacagt ttaccgtgat gttgcggcta cccacttcct tcgccaagga tttgctaaat
     7381 ccgacgacac cagcttttgc ggccgcgtag ttcacttggc ccgcgttgcc cattgatccg
     7441 actacggaag tgatattaat gatgcgacca aaatgggctt tcatcatcga gcgtataacc
     7501 agcttactca aacggaatac cgacttgagg ttggtctcca ttatttcgtc ccactccgtg
     7561 tccttcatgc gcaacagcaa attgtcccgg gtgattccgg cattatttac cagaattgcc
     7621 acggaaccat gctgggactc gatcgcagca aatacagctt gggtttccgc gccattattg
     7681 acgttgagtt tgtacccgtt acctttgaag ccggcgtccc gaagataggt tgatatgcca
     7741 gctgcgccgc tttcgctcgt tgcagttcct gcgatcgttg caccggcttc ggcgaggccg
     7801 agcgctatcg cctggcctat tcccctgctt gctccggtta tgagcacgac tttgccatcg
     7861 agcattggta tcccctactt cagcgcaatc aatgctgcct gaaaagccga ttcgtctgcg
     7921 aatgaaataa ccggtgtttc cggcgctatt cttttgttca gtcctgccaa aacttttccc
     7981 ggaccgcatt cgactatatg tgttgcgccg gcggataaca ttgcgcgaat cgtatcgacc
     8041 cagcgtaccg gctgataaag ttgccgcacc aaggcatctc gaagatcctg tactgagctg
     8101 gcgctccgga cagtggcatt atgaagtact ggaacggtcg acatcctgat agcgacgctc
     8161 tttagccttt ctcgcatgcg ctcggccgca ggccgcatca gactgcaatg cgaagttacg
     8221 ctcataggaa gcattatcgc tcgcttggct ccctttccct tggcaatgtc catagcgcgt
     8281 tcgaccgccc ctttatgtcc ggcgatcact acttgagccg gagaattgaa atttgcagct
     8341 tcaacggttt ggcctggctc gctggcttct gcgcaagcat cctcaacctc ggcatcctcc
     8401 aggccaagaa tcgccgccat tgcgccaaca ttctcgggta ccgcttcctg catcgcctgt
     8461 gctctataac gcaccagcga taccgcctcg gcgaagtcca tcgcatcccc cgccaccaag
     8521 gcgccatatt cgccgaggct atggcctgca gcgattattg gcaaagtccc gtaatgagac
     8581 ttccacgccc gatagagcgc tgtgtcagcc gccagcatta caggttgagt attgatcgtc
     8641 agactgagcg catcggccgg cccctcctga accagcttcc agaggtcgtc gccgaccaca
     8701 tcggaagcct cggagaaggt ttcccgcacc atggccagat ccgcaaagga tctcatcatg
     8761 ccaaccgatt gcgatccctg ccccggaaat acaaacgctg ctttcatatt taccagcgca
     8821 gcagcgcagc gccccaggta aagccaccac ctacgccttc cagcagcagc atctgaccgt
     8881 ccacgatgcg accgtccttc accgcttcgt ccagcgcaag gggaatggat gcagcagatg
     8941 tattgccgtg atgatgcacg gttacgatga ctttttcttt cggcaggcca agcttcctgg
     9001 cagtcgcttc catgatcctg atattggcct gatgcggaat aaaccagtca acatcggaga
     9061 ttgttaagcc cgccgcctgc agaacctcat tggccccttc ttcaaagacg cgaactgcaa
     9121 atttgaacac ggcgccgcca tccatgtgca cgaaaggcga accccatact tttccgccgt
     9181 ttacgctgcc cggtacacag agcatgtcct tgtggcggcc atcggcatgc agtctgcttt
     9241 caataattcc gggtttgtcg ctggccacca caatcaccgc gccagccccg tctccaaaca
     9301 atacgcaggt gccgcggtcg ctccagtcca aaatgcgcga gtacacttca gttccgacga
     9361 tcagtacttt tttcgctttg ccggcacgga cgaacaggtc cgcaatgctg agcgcataga
     9421 cgaacccgct gcagaccgcc tgcacatcga acgcgggaca accggccgcc tcgagcttcg
     9481 cctgcaggat gcacgcggta ctcggaaaga tcatgtctgg tgtcgtggtt gcaacgatga
     9541 tcaggtcaat ctccgccgca tccaggccag ccatcttcaa tgctcgttgc gccgccggaa
     9601 gcgccagatc gctggcaagc tggccgtcag ctgccacgtg acggctgcag ataccggtgc
     9661 gcgttcgaat ccattcatcg gaggtgtcca gcgtgcgggc gagatcgtca ttggtcacga
     9721 tccggtcagg caaataactt cctgtgccca caatacgaga gaacatcagg cggccctttg
     9781 atggatggtc gccaggcggt cgcttatgcc acgaagaaca tcgtggcggg cttcttcgta
     9841 cgcccgctgg atcgcaaaac gaaacgcgaa actatctgca gagccatggc tcttgaccac
     9901 aatgcccttg agaccgagca gactcgcgcc attgtatcgc cggtgatcga cgcgccgacg
     9961 aaaagcattg atcaccggca aggccaccgc acccgtgatt ttcgtgaata gattgcgctg
    10021 gaattcttca cgcagatagg ctgcgagcat ttgcgccagg ccctcggatg ttttgagcgc
    10081 gacattaccg acaaatccat cgcacacgat gacatcggtg gtgcccttgt aaatatccgt
    10141 gccttctatg tttccgtaga aattaagtcc gctttcccgc aacaagtccg ccgcctgctt
    10201 gactacttca ttgcccttta tttcttcttc cccgatattg agcaggccca ctgatgggcg
    10261 gtcgatatga tccacagcgc tgacaagaga agagcccatt acaccgaact gcagcagatg
    10321 attcgcgctg cagtcaacgt ttgcgcccag atcgagcaca caggtatgac ctcgtagcgt
    10381 tggcagaaag gacgcaattg ccggccggtc gatgcccggc agcatcttca gcacgaatcg
    10441 ggaaatggcc atcagcgcgc cggtgtttcc ggcgctgacg cacgcctgcg catcctgaat
    10501 tttcagcgca tcaatggcga tccgcatgga ggattcgcgt ttcccacgca aggcagatgc
    10561 aggcgcttca tccatggcga tggtctcggg tgctgaccgg atctggatac ggtcccggat
    10621 ggaagccgct gtgcccttta gttcggattc gagaatctca ggcaaaccga ccagaaggat
    10681 gcgcgtgtct tccgtctctg ccagaaatga aatcgcagcc ggcaccgtca cgtgcgcgcc
    10741 gtaatcgcca cccatgcaat caattgctac ggtagtcaat gcttattgga ccggtttatc
    10801 gcgacaacag cggacgatgc tcgcatgcaa tgcgcgtatt ttgcaccaga cagacctgga
    10861 aaccgggata tgacagggag cacagctgcg cgatatgggc agccaaagcc agcgagtcgc
    10921 tattcgccct tggtcttgac aacttttttg ccgcgataaa atccgttcgg actgatgtga
    10981 tgccgcagat gcgtttcgcc cgtagttggt tctactgcga gggtcggatt agtcaggaaa
    11041 tcgtgcgacc ggtgcatacc gcgcttggac gccgatttct tgttctgctg aactgccatg
    11101 ggttgctcct tataatctaa tcgtctaccg gcccttcagc gctgaaagcg aactgaaagg
    11161 ggacttcatt tcgttcccat cattcgcatc ttgtaatggc gaacaatccc ct
//
LOCUS       DATMZQ010000463         5274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2276484, whole genome shotgun sequence.
ACCESSION   DATMZQ010000463 DATMZQ010000000
VERSION     DATMZQ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276484"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VN496_14695"
     CDS             <1..317
                     /locus_tag="VN496_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HXF18253.1"
                     /translation="GLHTDDNRSVAVHNGRYGPYVKHGKTNATIPPSIDPSKITLEEA
                     LELLATKTAKAGKPKAGKQRKNAAKIQKDVNSSSTRKAAKPAIGARKKKRVAATGKRK
                     TG"
     gene            complement(324..2722)
                     /gene="gyrB"
                     /locus_tag="VN496_14700"
                     /pseudo
     CDS             complement(324..2722)
                     /gene="gyrB"
                     /locus_tag="VN496_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
     gene            complement(2744..3850)
                     /gene="dnaN"
                     /locus_tag="VN496_14705"
     CDS             complement(2744..3850)
                     /gene="dnaN"
                     /locus_tag="VN496_14705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXF18254.1"
                     /translation="MKILDIDKDVLLKPLQAVSGIVERRHTLPILSNVMLEVRGMDLD
                     LVATDLEIQISTSTKMDHAGSDHAVTVSARKLQDILRALPEKTLVTLDAQDGRLQVKA
                     GKSKFNLQTLPSQDFPRLAQGGSAMASVKMPQKVFRGLLSLVQYGMAQQDIRYYLNGL
                     LLVAEGSRLTMVATDGHRLAYASTALGGEVEKSEVIVPRKAILELAKLLQDVEEEVEI
                     QFLANQVRFIFSGIDVISKVVDGKFPDYTRVIPTNYKKHFSIGRVLLQQALQRASILS
                     NEKFRGVRWVLTPNSLRIICSNAEQEEAEEELNIDYVGDSLDIGFNVIYLLDVLNNLD
                     TDEIDCAVGDANSSMLISVAGNKDFKYVVMPMRI"
     gene            complement(4027..>5274)
                     /gene="dnaA"
                     /locus_tag="VN496_14710"
     CDS             complement(4027..>5274)
                     /gene="dnaA"
                     /locus_tag="VN496_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXF18255.1"
                     /translation="RLDGENVVVSAPNRFVLEWVRDKFLADIEKLSAEYLRKRVEVVI
                     NLGDKSVQVPDEAVEVSEAPNGAPPPKPKIREQTRLNPSFTFDSFVTGKANQLARAAA
                     LQVSEHPGVAYNPLFVYGGVGLGKTHLIQAIGNLLLEFNPAANVRYIHAEQYVSDVVR
                     AYQHKAFDGFKKYYHSLDLLLIDDIQFFNGKNRTQEEFFYAFNALIEAHKQVIITCDT
                     YPKEIAGIEDRLISRFGWGLTVAIEPPELEMRVAILLKKAEADRVILAEDVAFFIANH
                     IQSNVRELEGGLKRVLAYAKFANAKVSLDLAREALKDLLAVQIRQVSIDNIQRIVADY
                     YKIKVSDMYSKKRSRTVARPRQMAMALAKELTQLSLPDIGDAFGGRDHTTVLHACRKI
                     VDLRASNPEISRDFVALLQTLRG"
BASE COUNT         1246 a         1412 c         1289 g         1327 t
ORIGIN      
        1 tgggactaca taccgatgac aaccggtccg tcgctgtaca taacggacgc tacggcccct
       61 atgtgaaaca cggcaagacc aatgccacga tcccgccgtc catcgatcca agcaagatta
      121 cgcttgaaga agcgctcgag ttactcgcga caaaaacggc gaaggccggg aaacccaagg
      181 ctggaaagca acgaaagaac gcagcaaaaa ttcagaaaga tgttaattca tccagtacgc
      241 gcaaagctgc taaaccggcc attggcgcac gcaagaaaaa gcgagttgcc gcaactggta
      301 aacgcaagac tggttaacac gcattaaacg tccaggttac ggaccccgag agcgttcgat
      361 tcgatgaagg cgcgccgcgg ttctaccatg tcgcccatga gcgtcgtgaa gatttcatcg
      421 gcggtgatcg catcttcgat ttgcgtccgt aacagacgtc gtgacgcagg atccatcgta
      481 gtttcccaca actgctccgg gttcatttca cccagtcctt tgtatcgctg aatagcgacg
      541 cctttcttca cttcctcaag cagccaatcc aatgcctcct tgaagtcggc cacgcggtgt
      601 tcatgctcac cgcgcttgat gaaggcgcct tggctgatga gcccctgcag tacatgggaa
      661 gtttgtacta tctgagcata gtcaccactg ccaagaaaat cgcggtctaa atagccgact
      721 ctcaaatttc cgtgttgcat ccgtttgatg gccagtacct gcgtctcgtt tttcgagtcg
      781 taactacgct caatgaccag gcccggagcg cggcacaatt gcatgagctg ctgtgccgaa
      841 tggtcggccg cctccgcatt atccaaactg atctgagggt ccctgagcag ggcggacagt
      901 acaaccggat cgattcgctt tcccagccgc tctattaccg cctcggcgag aatgtaagag
      961 cgcgcaactt cttccagcgc gtcattcgcc agcgggcccc ggtctcctcc aggatgaagc
     1021 actgcgtctt tcagggccaa tcgcaacaaa tattctttta gctcgtgatc gtcctttagg
     1081 taacgctctt ccttgccatg tttggccttg tagagcgggg gttgcgcaat gtagatatgg
     1141 cctcgctcca ccagatcggg catctgccta tagaaaaatg tcagcaaaag cgtgcggatg
     1201 tgcgacccat caacatctgc atcggtcatg atgatgatac ggtgatagcg cagcttttca
     1261 ggtttgtagt catccttacc gatgctcgta cccagcgccg taatcaaggt tgcgatttcc
     1321 tgggacgata ccaatttgtc gaatctggcg cgttcgacgt tcagaatctt tcctttcagc
     1381 ggcaagatag cctgaaactt gcgatcgcga ccttgtttgg cagatccgcc tgcggaatcg
     1441 ccttcgacaa gataaagctc acagagcgac ggatctttct cctggcaatc tgcgagtttt
     1501 ccgggtagac cgaaaccttc aagcacaccc ttgcggcgag tcatctcgcg cgcctttcgg
     1561 gccgcctccc tggcacgtgc cgcatcaatg attttgctac gattacttta gcatccgctg
     1621 gcttttccag cagccaatcg tgaagcgtct tcgaaatcgc atcttcaacg accgggcgca
     1681 cttccgaaga aaccagcttg tccttggtct gcgatgaaaa tttcggatcg ggcaacttca
     1741 ccgacagaat acacgtaagc ccctcgcgca tatcatcgcc agtcgtttca actttggctt
     1801 tcttggcaag ctcgttggat tcgatgtact ggttcaacgt gcgcgtcatg gccgcgcgca
     1861 aagccgtcaa atgcgtaccg ccatcgcgtt gtggaatatt gttggtgaag cacaggaccg
     1921 attcctgata tgaatcgttc cactgcattg cgacctcgac accgataccg agctgttcga
     1981 acacggcatg gaaaatattc gaatgcagaa cagtcttgga cctgttgatg tactccacga
     2041 atcccctgac acctcccgaa aaagcgaaaa cttcttcctt tccagtccgc tgatcctgta
     2101 attcgatacg cacaccgttg ttcaggaaag acagttcgcg gagccgcttt gccagtatct
     2161 cgtagtggaa ttcgatgttt ccaaaaactt ccgggctggg atgaaaatgg atttctgtgc
     2221 cgcggcgctc tgtggttccg attaccgtca agggcgccag gggatctcca ttgcgaaact
     2281 ccattgaatg cacttgccca tcgcgccgga tggtcagttt tagggtatcg gaaagggcat
     2341 tgacaacgga cacaccgacc ccgtgcagac cgccggaaat tttgtagcta ttgctatcga
     2401 acttgccgcc ggcatgcaga accgtcatga caatttcggc tgtcgatcta cccgcgtcat
     2461 cttccggatg aagccctgtt ggaatgccgc ggccattgtc gagcacgctt accgaattgt
     2521 cggtgtggat tacgaccttt atttcgtcac agtggccggc tagcgcttcg tcgatcgaat
     2581 tatcgaggac ttcaaagacc atgtgatgta atccggtgcc gtcactcgta tcaccgatgt
     2641 acataccggg tcgcttgcga actgcctcca ggccctttag tactttgata ctgtcggaat
     2701 tatattcaga tgatgaagac attaaaaaaa tctatggtta tggtcagatc cgcatgggca
     2761 tcacgacgta cttgaagtct ttgttgcctg ccaccgaaat cagcatgctg ctgttagcat
     2821 ctccaacggc gcagtcaatt tcatcggtat ccaggttgtt taacacatcg agcaaataaa
     2881 tgacattgaa cccgatatcg agcgagtcgc cgacataatc aatgttcaat tcctcttcgg
     2941 cttcctcttg ttccgcatta ctgcaaataa tcctgagact gttcggagtc agtacccagc
     3001 gtaccccgcg gaacttttcg tttgacaata tcgaggcgcg ctgtagcgcc tgctgcaata
     3061 aaacacggcc aatcgaaaaa tgcttcttgt aattggttgg tataactctc gtgtaatccg
     3121 ggaacttgcc gtcaacgact ttggaaatta catctattcc ggaaaatatg aaccgcacct
     3181 gatttgccag aaactggatt tccacttcct cttcgacatc ttgcaggagc ttggccaatt
     3241 caaggatcgc ttttcgtgga acgatcactt ccgatttttc gacttctccc ccaagcgcag
     3301 ttgatgcata agcgagccga tgtccgtcgg ttgctaccat agtcaaccgc gaaccctcgg
     3361 caaccaacag caaaccgttc agataatatc gaatgtcctg ttgtgccata ccgtattgca
     3421 ccagggacag cagcccgcga aatacctttt gcggcatttt cacgctggcc attgcagaac
     3481 cgccttgggc cagccgtggg aaatcctgcg aaggcagcgt ttgcaaattg aatttgctct
     3541 tgccagcttt tacttgcagg cggccgtcct gagcgtcgag cgtcacgaga gttttctcgg
     3601 gaagtgcacg caagatgtct tgcaattttc gtgcggaaac cgttacggcg tgatcggacc
     3661 ctgcgtggtc catttttgtt gacgtcgaaa tttgaatttc aagatccgtg gccaccagat
     3721 cgagatccat tcccctgact tccaacatga cgttggaaag aatcggcaat gtatggcgac
     3781 gttcgacaat tcctgaaacc gcctggagcg gcttcagcaa cacatccttg tctatatcca
     3841 agattttcat tctttaaacc ttgataacaa taatagagtt gggcaagtcg gttgattcaa
     3901 gcgtaaatgc catattaagc atgcagatcg cgcaatgtat aagtgaagtt atgccagtgg
     3961 atggattgtg gatgaatacc gtttcacccg cttttgacta gttttcaaca atgcatcccg
     4021 cggttatcaa ccgcggaggg tttgcagcag cgcaacgaaa tcacgtgata tttccggatt
     4081 actggcacga aggtcaacga ttttccggca tgcatgcaac actgtcgtat gatcgcggcc
     4141 gccaaaagca tctccaatat cgggaaggct tagttgggtc aattccttgg cgagagccat
     4201 tgccatttgc ctcgggcggg cgaccgttcg agaccttttt ttggaataca tatcggatac
     4261 cttgattttg tagtaatccg cgacaatgcg ctgaatgtta tctattgaaa cctgccttat
     4321 ttgcacggcc aaaagatctt tcagcgcttc tcgtgcaaga tccagggaaa ccttggcgtt
     4381 tgcgaatttt gcgtaggcga gcacccgctt gagtcccccc tcaagttccc gaacgtttga
     4441 ctggatgtga ttggcgatga aaaatgcgac atcctccgca aggatcacgc ggtcagcctc
     4501 tgcctttttc aatagtatgg ccactctcat ctccaattca ggcggttcaa tagcgaccgt
     4561 taatccccaa ccgaagcgtg aaatcaaacg gtcttcgatg ccggcgatct cttttggata
     4621 tgtgtcgcac gtaatgatga cttgtttgtg tgcttcgata agcgcattga aggcataaaa
     4681 gaattcttcc tgcgtgcgat tttttccatt gaagaactgg atgtcatcga tcagcagcag
     4741 atcgagtgaa tgatagtatt tcttgaagcc gtcaaaggcc ttgtgctgat aggctcgcac
     4801 cacgtccgag acatactgtt ccgcatggat atatcggacg ttggccgcag gattgaattc
     4861 gagcaacaag ttaccaatgg cctggatcaa atgcgtcttg ccgagtccga cgccgccata
     4921 aacaaaaagc gggttatagg ctacgccagg atgttccgaa acctgcaatg ccgccgcacg
     4981 tgcgagctga tttgccttcc cggtaacgaa cgagtcgaat gtaaaagacg ggttgagccg
     5041 agtttgttca cgaatttttg gtttgggcgg tggtgccccg ttcggggcct cactaacttc
     5101 aacagcttcg tccggcacct gcacggactt gtcacccaga ttgatcacga cttcgacccg
     5161 tttgcgcagg tattcagcag agagtttttc gatgtcggct aaaaatttgt ccctgaccca
     5221 ttccaatacg aatcggttcg gggccgatac cactacgttc tcgccgtcta gacg
//
LOCUS       DATMZQ010000464         2998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_933467, whole genome shotgun sequence.
ACCESSION   DATMZQ010000464 DATMZQ010000000
VERSION     DATMZQ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933467"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VN496_14715"
     CDS             <1..959
                     /locus_tag="VN496_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF18256.1"
                     /translation="PGNLQAYSDTPIYARTPGYLKRWHADIGKRVKKGELLAEIDAPE
                     LDDQLQQAKADYATAQANYQFAQSTAVRWEALLKTDSVSRQEADEKRNDMAARKAVMD
                     AARYNVQRLEKLQSYKRIYAPFDGVITARNVDVGMLIDAGGGSAKEMFHIASTTRLRA
                     FVNVPQSYARDVGPGMTAELTLSEFPGRRFKGTFARSSQSIDVATRTLLSEVDVDNAS
                     GELMPGSYAQVHLRLSSAHDGALIVPVNTLLFRPESVMVAVLQNDHAVLTPIKIGRDF
                     GSEVEVVSGLSANDSIILNPSDSLVSGAQVRVVQEPAPKAKG"
     gene            1001..2482
                     /locus_tag="VN496_14720"
     CDS             1001..2482
                     /locus_tag="VN496_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXF18257.1"
                     /translation="MRVGVATLMLGMAAACTVGPDYVKPEVTVPAGYKEAAADGIAWK
                     VAEPGDRTGRGKWWSVYEDPLLNALQDVVTTSNQDLRIAQSRYTQARALAAAANSNYL
                     PTVTAGASVIRFRNSASSFGATSTNIGPATAYRLPLEVSWELDLWGRVRRTVESSEAS
                     AQAVAADLETVRLSLQAELALDYFLLRSLDSERQLLERTLIAYRKAYDLTVSRHVGGA
                     ASDADVAQAETQLKTAEAQAVNLRVQRAQLEHAIAVLIGKAPAEFSMPQAGLESLPPS
                     VPLVMPSELLERRPDISSAERQMASANAQIGVAKAAFYPTISIGASAGFEGAKGSDWF
                     NWPSRFWSVGPSAALILFDGNRRRSVSEQAQAAYEGTVATYRQTVLNAFQDVEDNLAE
                     LAILAEEAGIQDAAVKASRRSVAQTSNRYEAGAASYLEVVVAQTFALTNERAAVDISR
                     RRMLASVRLIKALGGNWSVADLPSGDGKVAMKKDNQPVQTQTK"
     gene            complement(2506..>2998)
                     /locus_tag="VN496_14725"
     CDS             complement(2506..>2998)
                     /locus_tag="VN496_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXF18258.1"
                     /translation="ATLPDRVLAHLKLLGGDTGGFAVDRLQHSLLTATLAHKGGEDEE
                     YVVCALLHDIGDTLATWNHPDVGAAIIKPFVSEANHWMVEKHGIFQGYYFFKHIGLDG
                     NMREQFRGHPHFERTARFCEIYDNPAFDATLECAPLEFFEPMLRRVFAAPKNSIYKDA
                     CQA"
BASE COUNT          611 a          868 c          929 g          590 t
ORIGIN      
        1 ttccggggaa cctccaggcg tattccgaca cgccgattta cgccagaacg ccgggctatc
       61 tgaagcgctg gcacgccgac atcgggaagc gcgtaaagaa aggtgagcta ttggccgaaa
      121 tcgacgcgcc agaactggat gaccagctcc agcaggcgaa ggcggactac gccaccgcgc
      181 aggcgaacta tcaattcgcg caatccactg ccgtacgttg ggaggcattg ctgaagactg
      241 attcggtgtc gaggcaggaa gccgacgaaa agcgcaacga catggcggcg cgcaaagcgg
      301 ttatggatgc cgcgcgttac aacgtccagc ggctcgaaaa actgcaatcg tacaaacgca
      361 tctatgcgcc gttcgatggg gtgatcactg cgcgcaacgt cgatgttggc atgctcatcg
      421 atgccggcgg tggatctgcg aaggagatgt tccatatcgc ttccaccacg cgcttgcgcg
      481 cgtttgtcaa tgttccgcag tcctacgcgc gcgacgtggg ccctggcatg acagccgaat
      541 tgacactctc ggaatttccc ggacggcgct tcaaaggcac attcgcccgc agttcacagt
      601 ccatcgacgt ggcgacccgc acgctacttt ctgaagtcga cgtcgataac gcttccggtg
      661 aactgatgcc cggatcgtat gctcaggtgc atttgaggct gtcctccgcg cacgacggag
      721 cgctgatcgt gccagtgaat acattgctgt tccggcccga aagtgtgatg gtcgccgtac
      781 tgcagaacga tcatgccgtg ctgacgccca tcaagattgg ccgggacttc ggctccgagg
      841 ttgaagtcgt ctccggcttg agcgcgaatg actccatcat tttgaatccg tcagattccc
      901 tcgtttccgg agcccaggta cgcgtcgtgc aagagccggc accgaaagcc aagggataag
      961 gaacatgtgc cgcgtcgtga actgcgggaa aatgcacgcc atgcgcgttg gcgtagccac
     1021 gctaatgctt ggcatggcag cggcctgcac agtgggtccg gattacgtca agcccgaagt
     1081 aacggttccg gccggttaca aggaggcggc cgcagacggg atcgcctgga aagtggccga
     1141 acccggagat cgcaccggcc gcggaaagtg gtggagcgtt tatgaagatc cgctgctgaa
     1201 cgcgctgcag gatgtcgtga ccacgtcgaa tcaggacctc cggatagcgc aatcgcgcta
     1261 tacgcaggcg cgtgctctcg ccgcagcagc caatagcaat tatctgccga ccgtgaccgc
     1321 cggtgccagc gtgatccggt ttcgtaattc ggccagcagc ttcggcgcca catcgaccaa
     1381 tatcggcccg gccacggcat atcgtcttcc actggaagtt tcctgggagc tcgatctatg
     1441 ggggcgtgtg cggcgtacgg ttgaatctag tgaagccagc gcgcaggccg ttgccgccga
     1501 tctcgaaacg gtgcggttga gtctgcaggc tgagctcgca ctcgattatt ttctgctgcg
     1561 cagcctcgac agcgaacggc aattgctgga gcgcacgctg attgcgtatc gcaaggccta
     1621 tgacttgacc gtcagccgtc atgtaggagg cgccgcatcc gatgccgatg ttgcccaggc
     1681 agaaacgcag ctgaagactg ccgaggcgca ggcagttaat ttgcgtgtgc agcgtgctca
     1741 gctcgagcac gcaatcgcgg tcctgattgg caaagccccg gctgaatttt caatgccaca
     1801 ggcggggctg gaatcgctgc cacccagcgt gccgctcgtg atgccgtcgg aattgcttga
     1861 gcggcgtccg gatatttcct ccgcggaacg gcaaatggca tcggccaatg cgcagatcgg
     1921 tgttgcaaag gcggccttct atccgactat ctcgatcggt gcaagcgccg ggttcgaggg
     1981 ggccaagggg tccgactggt tcaactggcc aagccgcttc tggtcggtcg gcccttctgc
     2041 tgccttgatc ctgttcgacg gcaaccgccg acgctcggta tcggaacagg cgcaggcggc
     2101 ctatgagggc acggttgcca cgtatcggca aactgtgctg aatgctttcc aggacgtgga
     2161 ggacaatctg gcggaactgg ccattctggc cgaagaagcc ggcatccagg acgccgcggt
     2221 gaaagcgtcg cgacgttcgg ttgcgcagac ttcgaatcgt tatgaggctg gcgcagcgag
     2281 ttacctcgaa gtcgtggtcg cgcagacgtt tgcgctgacc aatgaacgcg cggcggtgga
     2341 tatttcgcga cggcgcatgt tggccagcgt gcgcctgatc aaggcgctgg gtggtaactg
     2401 gagcgtggcg gatctcccct ccggcgacgg caaagttgcc atgaaaaaag acaaccagcc
     2461 ggttcaaacc cagacgaagt aggtcagttg ctttttcgtt gcgcgctacg cctggcacgc
     2521 atccttgtaa atggaattct tcggcgccgc gaacactcga cgcaacatcg gctcgaaaaa
     2581 ttcaagcggc gcgcattcca gcgtggcatc gaacgcgggg ttgtcataga tctcgcaaaa
     2641 gcgcgcggtg cgctcgaaat gcggatggcc gcggaattgc tctcgcatgt ttccatccag
     2701 cccgatgtgc ttgaagaagt agtagccctg gaagatgccg tgcttctcga ccatccagtg
     2761 attcgcttcg gatacgaacg gctttatgat ggccgccccg acatcaggat ggttccatgt
     2821 tgccagcgtg tcgccgatgt catgcagcaa tgcacagacg acatattcct cgtcttcccc
     2881 tcctttgtgt gcaagggtcg cagtcagcag cgaatgttgc aggcgatcga cggcaaagcc
     2941 gccggtatct ccccccagca atttcagatg tgcgagcacg cgatcgggca gcgtggcg
//
LOCUS       DATMZQ010000465         3369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3695229, whole genome shotgun sequence.
ACCESSION   DATMZQ010000465 DATMZQ010000000
VERSION     DATMZQ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695229"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VN496_14730"
     CDS             complement(<1..406)
                     /locus_tag="VN496_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18259.1"
                     /translation="MRALSSQAQSIGLFFGSRIGPAPDLMAASKFWIHHTARVDPAPY
                     IEAAERIAAGRLDVFALRDLEVGTPPLWNRDPKSGVEAPCSFGKLLDYRNPQLVGDIK
                     YLWEPNRHLHLVTLAQAYVLKHDPLYLQVIRQH"
     gene            complement(1179..2285)
                     /locus_tag="VN496_14735"
     CDS             complement(1179..2285)
                     /locus_tag="VN496_14735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXF18260.1"
                     /translation="MITGSTMGKHCVVVLGPSLHAVSGVSTHVRMLISSGLARDYDLR
                     HFRVGSEGRRENALQKVMRFALSPAHLALFLLQTGAEVVHLNASLDAKGFWRDLAYWI
                     IARLLGRRVVNQIHGGAMPQEFFHGNASLTWILRRFLVSSDVVTVLSSAELAAYRAFD
                     PRIRVHLVPNAIDPVGQADQARSYNTDQPLKLLYVGRILRAKGLFELIDALAELKRTG
                     RDFRLSIAGSGPDQAELMAAAQRHSLLDRVQFLGGVFASAKARLWLTSDLFVFPTHRE
                     GLPYSLLEAMAAGCVPITTAVAAIPDVMRDGEHGMFVPVRDAPALAAAIATLDDHRER
                     LIQMGEASRRRVLEQYTVARLADDFRRLYSGCLS"
     gene            complement(2298..>3369)
                     /locus_tag="VN496_14740"
     CDS             complement(2298..>3369)
                     /locus_tag="VN496_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HXF18261.1"
                     /translation="EFSELRHARKVARRYGTEHHELTVGFRDFAEELPLLAWIYDEPM
                     GEPPGIPTYFMCRAAKKHVTVMLCGEGADEQFGGYSKYIFDQFSSKLDWMPSAIRGSL
                     LRAMASGLPFGGRRLRSMAEILAISDMPSRFASWYGGFDTRHQTRVLSDKMQNEIGDG
                     GLAHAFHEIVNACDSKNALDRFLYCDIHSRLVDDILVKSDRMSMGASIEARVPFLDHK
                     VVEFAARLPQNMKIDGLRSKIVLKRLGERYLPHEIMYRRKVGFTVPLSRWFTGPWRGL
                     ITDVLLSDRCLDRGYYNADVVRSIVDDHIERRIDREQGIWIMLALEVWHRLFVDDDGS
                     EAAVDRIKEDFVCSLGSLQAAA"
BASE COUNT          704 a         1012 c          957 g          696 t
ORIGIN      
        1 ggtgttggcg aatgacctgg agatagagcg gatcgtgttt gagcacgtat gcctgcgcca
       61 gcgtaaccag gtgtagatgc ctgttgggtt cccataggta cttgatatcc cctaccagct
      121 gagggttgcg gtagtcgagc agcttgccga agctgcaggg cgcctctacg ccggacttcg
      181 gatcacggtt ccagagtggc ggcgtaccaa cctcgagatc gcgcagcgca aagacatcga
      241 gcctgcccgc agcgatgcgc tcggcagcct cgatgtacgg cgccgggtct acccttgccg
      301 tgtgatggat ccagaacttc gacgcggcca taagatcggg ggcgggcccg attctcgacc
      361 cgaagaacaa tccgatgctc tgcgcttgcg atgatagggc gcgcatcagc cgatagggta
      421 tctccgccgg cgtcatgcag cgcaggcgat taaagtgcca tacccaccag tcccttcccc
      481 aggccgcaga actatgctta agacgatgtc caatgtctaa gaaatcgtgt gcgggcatct
      541 tgtctcgaac aactctctga attgaagcgg cgccactgca attgcccgcg aacttaagat
      601 gggatcgccg ccagaattct ttcaaccgtg cactcgtgag cgagcgcttc agtctggcaa
      661 ttcagccggg gttcgctctt atgttgacgt tgcgctgcag actcggaatg gaatttcgcg
      721 ggcgccacga cgcctacgaa aagcaaggcg tctggattca cgaagaatgg cgttccaatg
      781 gacttggttg atctgcgcgt cacctgcttg tcccgggcag tggaaggttc ggtggattga
      841 gcacggcttc cagtatgcaa gcgcccgcat ttccagcctt ggattgtcgt tgccggccga
      901 cgtgatccga ttggattaat gcatgatcgc agggcgccgt gccaaagcaa tgcgttgccc
      961 gttcggtttg ttgatcgcat cgttcacctt aaaaaaaatt acgcgccagc tttgtgccca
     1021 agagtttccg gctggttgag cgttgtgcag ctatcacgat gcgggttcat tccagaacgg
     1081 atgatcccgt ccatgccgct ccgcttcatc agactctgcc atcttgcggc gacaaacatc
     1141 cgagcactca gccgaacccc atcgacgcaa tccgcgtttc aacttaggca gccgctgtat
     1201 agcctgcgga aatcatccgc cagtcgtgcc accgtatatt gctccagcac tcgcctacgt
     1261 gatgcttccc ccatttgaat caggcgttcg cgatggtcat ccagagtagc tattgcggcc
     1321 gcgagcgccg gtgcatccct cactggtacg aacataccgt gctccccgtc gcgcatcaca
     1381 tccggaatcg cggcgacggc ggtagtgatg ggaacgcagc ctgccgccat ggcctccagc
     1441 aacgaataag ggagcccctc cctgtgggtc ggaaaaacaa acagatcgga cgtgagccaa
     1501 agtcgggcct ttgccgaggc gaacacgccg cctagaaatt gcacgcgatc gagcaggctg
     1561 tgcctttgcg cagcagccat cagttccgcc tgatcaggac cagaccctgc gatcgacaac
     1621 ctgaaatctc gcccggtgcg tttcagctct gccaaagcgt cgatcagttc gaacaatccc
     1681 ttggcgcgca ggatccgacc gacataaagc agcttcaatg gttggtcggt attgtatgag
     1741 cgtgcctgat cggcttgccc gactgggtcg attgcgttgg gaaccaaatg gactctgatg
     1801 cgtggatcga acgcgcggta ggcagccagt tccgcactgg agagtacggt gactacgtca
     1861 ctcgatacca ggaatcgccg caagatccag gtcagtgagg catttccgtg aaaaaactcc
     1921 tggggcatcg ccccgccgtg gatctggttc acgacgcgac ggccgagcaa cctggcaatg
     1981 atccagtacg ccagatcgcg ccaaaaacct ttcgcatcca aagaagcgtt gagatggacg
     2041 acctcggcgc cggtctggag caggaacagc gcgagatgcg cggggctaag cgcgaagcgc
     2101 atcaccttct gcagtgcatt ttcccggcgc ccctcgctgc caactctaaa atgccgcaga
     2161 tcgtagtcac gcgccagccc ggaagaaatc agcatccgta cgtgggtcga cacgccgctg
     2221 acagcatgca gtgaaggacc taacacgact acgcagtgct tgcccatggt cgatccagtg
     2281 atcatgcgtc gcgcaatcta tgccgctgct tgcagcgagc ccagggagca cacgaaatct
     2341 tccttgatcc tgtccaccgc cgcttcactg ccgtcatcgt ccacgaataa acggtgccac
     2401 acttccagcg cgagcattat ccagattccc tgttcgcggt cgatgcggcg ctcgatatgg
     2461 tcgtccacta tgctgcgcac cacgtcggcg ttgtagtagc cgcggtcaag gcatcggtcg
     2521 gaaagcagta catcggtgat caatccgcgc cacggtccag tgaaccagcg actgagcgga
     2581 acggtgaatc ccaccttgcg gcggtacatg atctcgtggg ggaggtatcg ttcgccaagc
     2641 cgctttagca cgatcttcga acgcaatccg tctatcttca tattttgcgg caggcgcgcg
     2701 gcgaattcga ccaccttgtg gtcgaggaac ggcacgcgag cctcgatgct ggctcccatg
     2761 ctcattcggt cgcttttgac caggatatca tccaccagtc gcgagtgaat gtcgcaatag
     2821 agaaaacggt ccagggcatt tttgctgtcg cacgcattga cgatctcgtg gaatgcatgg
     2881 gccaatccac cgtccccgat ttcgttttgc atcttgtccg acagcacgcg ggtttgatgc
     2941 cgcgtgtcga aaccaccgta ccacgatgcg aagcgcgacg gcatgtcgga aatcgcgagg
     3001 atctctgcca tgctgcgcag gcgccggcca ccaaatggca agccgctggc catggcccgc
     3061 agcagggaac cgcgaatcgc acttggcatc cagtcgagct tgctggagaa ctgatcgaat
     3121 atgtacttgg aatagccgcc gaattgttcg tcggcccctt ctccgcatag catgacagtc
     3181 acatgcttct tggccgcccg gcacatgaaa taggtcggta ttcccggagg ctctcccatc
     3241 ggctcgtcgt atatccaggc cagcagtggc aattcctctg caaaatccct gaagccaacg
     3301 gtgagctcgt gatgctcggt gccgtaacgt cgcgccacct tgcgtgcgtg ccggagctcg
     3361 ctgaattcc
//
LOCUS       DATMZQ010000466         7364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5040709, whole genome shotgun sequence.
ACCESSION   DATMZQ010000466 DATMZQ010000000
VERSION     DATMZQ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5040709"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /gene="tadA"
                     /locus_tag="VN496_14745"
     CDS             <1..318
                     /gene="tadA"
                     /locus_tag="VN496_14745"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HXF18262.1"
                     /translation="DPSAHAEILALRAAASALENYRLTNCTLYVTLEPCAMCAGAIQH
                     ARISRLVFGASDPKTGACGSVVNLFAEPLLNHHTQVQGGLLAKDCGELLSTFFRQRRM
                     APQ"
     gene            315..806
                     /locus_tag="VN496_14750"
     CDS             315..806
                     /locus_tag="VN496_14750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXF18263.1"
                     /translation="MKSIRISIQDQLLEFNEDGKPVRRYPVSTAKNGAGEKYGSFCTP
                     RGRHIIRAMIGAGEPLNTVFVKRRPTGEIFDDALGARFPGRDWMLTRLLWLSGCEPGF
                     NRLGNVDTMRRYIYIHGSPDSAVMGIPGSIGCVRMHNHDLLELFDMVQPGTPVEILEF
                     RAS"
     gene            885..1625
                     /gene="fabG"
                     /locus_tag="VN496_14755"
     CDS             885..1625
                     /gene="fabG"
                     /locus_tag="VN496_14755"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXF18264.1"
                     /translation="MRLKDKVAIITGSARGIGHATALKFAAEGARVVVCDLGSQAIDA
                     VVAQIIAAGGKAIGFTVDVTDKAGIAAMVKGVMDAYGRIDVLVNNAGIVDDAMLRKMS
                     DEQFERVIDINLKGTYNCARAVVDIMISQNAGVILNASSVVGIYGNFGQTNYAASKFG
                     VIGMAKTWARELGRKGIRANAVCPGFVETTILKSIPAKVMQAMIDRVPLGRLAKPEEI
                     ANTYAFLASDEASYINGAVIEVSGGATV"
     gene            1645..2076
                     /locus_tag="VN496_14760"
     CDS             1645..2076
                     /locus_tag="VN496_14760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF18265.1"
                     /translation="MKTSADIAVRLACWNSDREILSKIREEVFVKEQSVPADLEQDEF
                     DVKSQHVVALVGGVPIGTGRLLPNGHIGRMAVLRDWRRMGVGAAMLRALMNIARGLGL
                     RRVILNAQLHALPFYVHHGFQPVGDEFMDAGIPHRRMERDL"
     gene            complement(2073..2876)
                     /locus_tag="VN496_14765"
     CDS             complement(2073..2876)
                     /locus_tag="VN496_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HXF18266.1"
                     /translation="MCLILIAYQTDARYRLVVAANRDEFFRRPTAPADYWPDAAHILG
                     GRDLEKGGTWMGVATDGRWAAVTNFRDGSNARPGARSRGELVADYLRDPALPGTYADS
                     VRHGARDYAGFNLVLGNHQHLHYYSNQQTATMMLGPGVYGLSNGLLDTPWPKVERGKS
                     ALSGAIASSQGPDGLVESLLAVLSDRSIAPDDALPNTGIGKDWERRLSASFITAPGYG
                     TRASTVLLLTGDGEIHFRERCFGERAELLEDRRFRFVPSLTDSSVRPAS"
     gene            complement(2876..3151)
                     /locus_tag="VN496_14770"
     CDS             complement(2876..3151)
                     /locus_tag="VN496_14770"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HXF18267.1"
                     /translation="MALSYYIYYRVARPAQAEALVRDVQAELKTRCGIAGRLVRKRDD
                     ATTWMEIYEGVRDAAAFEHCLALSVQAAHFQNVLEPASARHMECFED"
     gene            complement(3157..4146)
                     /locus_tag="VN496_14775"
     CDS             complement(3157..4146)
                     /locus_tag="VN496_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18268.1"
                     /translation="MGRLMDWNDIVKQNAGSVPHFGEPREQYAAARSNSIVTPLRQFE
                     LLRFHGSDAKAFLQGQLTIDLDEVTSEQAQFGGYCNPKGRLIANFLLFVASSGYLMQV
                     PREVAGDLATRLRKYVLRSQVSIEHERGQELLGLAGPQAVTLLEEKQSAPPSRRMAVG
                     IRAHRSVIRLPGNQFVVVAASRDMSALWDELAERAVPAGEQCWAWLQIEAGIPWITQA
                     TREQFLPQMVRLDEIGGVSFNKGCFPGQEIVARTRYLGDVKRKLKLGHTRGEVQSGDA
                     LFSADQQTGTVLNAAPVPDAGCALLAVVSQPDTGQTRTADGESIVWSEAFQPD"
     gene            4193..4651
                     /locus_tag="VN496_14780"
     CDS             4193..4651
                     /locus_tag="VN496_14780"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HXF18269.1"
                     /translation="MHVPRLSLQIKASRQLAIALVLLHSAAIACVVWFLPRAWLSAGA
                     AIAILASLVFHLRRDALQLSGNSVTAFTLKEGAQCVLTFRNGGTLSGRIEGFSFTAPL
                     LTVIQVRPATRWGGRAAILMPDSASQQELRQVRVWLRHGAQLDMADSRNS"
     gene            complement(4637..6208)
                     /gene="nadB"
                     /locus_tag="VN496_14785"
     CDS             complement(4637..6208)
                     /gene="nadB"
                     /locus_tag="VN496_14785"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HXF18270.1"
                     /translation="MKRFDVAIVGSGLAGLTVALHLADHRKVGIFTKRELLDGASNWA
                     QGGIAAVLAKDDSPGDHIRDTEIAGAGLCDAATTRYVVEHGRQAIEWLIGQGVPFTRD
                     TSDLGYHLTREGGHSHRRVIHAADATGHAVQSTLEGKIKAHPNITVLEQYMAVDLITA
                     EKLGLAEKRCVGVYCLDRQSGRVETIAADQVVLATGGAGKVYLYTTNPDTATGDGIAM
                     GWRAGCRIANMEFIQFHPTCLYHPHAKSFLISEAVRGEGGILRLPDGTRFMPAHDQRA
                     ELAPRDVVARAIDFEMKKRGLDCVYLDISHKPAEFLREHFPNIYSRCLELGIDISRQP
                     IPVVPAAHYTCGGIQVDRDARTDLAGLYAVGETAHTGLHGANRLASNSLLECLVFGQT
                     AATHILQQAKLPSTALPRWDESRVTDADEEIVISHNWHELRRFMWDYVGIVRTNKRLE
                     RARHRISLLTDEIQEYYSNFKVSADLLDLRNLVTTADLIVRSAMRRHESRGLHFSLDY
                     PGLLSKAEDTVLRIA"
     gene            6426..7025
                     /gene="rpoE"
                     /locus_tag="VN496_14790"
     CDS             6426..7025
                     /gene="rpoE"
                     /locus_tag="VN496_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HXF18271.1"
                     /translation="MGDREVDQALVERAQRGDKQAFELLVAKYQRKLGRLLSRFIRDP
                     AEVEDVAQEAFIKAYRALPSFRGDSAFYTWLYRIGINTAKNYLVAMGRRAPTTTEYDS
                     EEAENFEDGDQLRDLNTPEAELMTKQIASTVNQTMEELPEELRTAITLREIEGMSYED
                     IANVMNCPIGTVRSRIFRARETIAERLRPLLDTSKEKRW"
     gene            7028..>7364
                     /locus_tag="VN496_14795"
     CDS             7028..>7364
                     /locus_tag="VN496_14795"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HXF18272.1"
                     /translation="MEKISALMDGEIGGAEAAVQIKRLKEESQFRAAWDTYHVIGDTL
                     RGEKLWLSQRFTESFSQRLAQEPTVLAPQVRSPLQTAMRRFALPVAASVGGMMLVAWL
                     ALFNNPFAPP"
BASE COUNT         1540 a         2122 c         2162 g         1540 t
ORIGIN      
        1 gatccgtcgg cccatgcgga aatcctggcc ctgcgtgccg ctgcctcagc gctggaaaac
       61 taccgcctca cgaattgcac gttgtatgta accctggaac cgtgtgccat gtgcgccggg
      121 gccatccagc atgcgcgcat ctcgcgttta gtattcggcg catccgatcc aaagaccggg
      181 gcttgtggca gcgtggtgaa cctgtttgcg gaacccttgc tgaaccatca cacacaagtt
      241 cagggcggcc tgttggcaaa agattgcggc gaactgcttt cgactttctt ccggcagcgc
      301 aggatggcgc cccaatgaag tcgatccgca tcagcattca ggaccaactg ctggagttca
      361 atgaggacgg caagccggtc aggcgctatc cggtgtccac cgcgaaaaac ggcgcaggcg
      421 aaaagtacgg cagtttctgt accccgcggg gccgccatat catcagagcc atgatcgggg
      481 cgggtgaacc gctgaacacg gtttttgtaa aacgccgacc gaccggtgaa atcttcgatg
      541 acgcactagg tgcccgcttt cccggtcggg actggatgct aacgcggctc ctttggctat
      601 cggggtgtga gccggggttc aacaggctgg gcaatgtcga taccatgcgt cgatacatat
      661 acattcacgg cagtcccgat tctgctgtca tgggaatccc tggttcgatc ggctgtgtac
      721 gaatgcacaa tcacgacctt ctggaattgt tcgacatggt ccagccgggc actccggtag
      781 agatcctgga attccgagcg tcctgaccgt tacgttcggt tgtgcatcgc agcgcaattg
      841 ggtacagtgc cgccggccca tgcgggcgaa aatggagttg tccgatgagg ctgaaggata
      901 aggttgcaat tatcacagga tcggcgcgcg gtattggaca cgccaccgca ctcaagtttg
      961 ctgccgaggg cgcccgcgtg gtcgtttgcg acctcggaag ccaggcaatc gacgcagtcg
     1021 tcgcccagat cattgccgcg ggtggcaaag ctatcggctt tacggtggac gtcaccgaca
     1081 aggcgggcat tgccgccatg gtcaaaggtg tgatggacgc ttacgggcgc atcgatgtgc
     1141 tggtaaacaa tgccggcatc gtcgacgatg cgatgctacg caagatgtct gatgaacaat
     1201 tcgagcgagt tatcgatatc aacctgaagg gcacctataa ctgtgcccgg gcggtagtcg
     1261 atatcatgat cagccagaac gccggcgtga tcctgaatgc gtcttcggta gttggcattt
     1321 acggcaattt cggacagacc aattatgctg caagcaaatt cggcgtcatt ggaatggcga
     1381 agacctgggc cagagaattg ggcagaaagg gcatccgtgc caacgcggtt tgtccggggt
     1441 tcgtcgaaac caccatactg aaatcgatcc ccgcaaaggt catgcaggca atgatagacc
     1501 gggtgccact tggtcgcctg gcgaaaccgg aagagattgc caacacgtat gccttcctcg
     1561 cttcggacga ggccagctac atcaacggtg ccgtaatcga agtcagcggc ggcgctacgg
     1621 tctagaggcg gctaggcggg gccgatgaaa acctctgccg acattgcggt tcggctcgca
     1681 tgctggaaca gcgaccgtga aatcctcagc aaaattcgcg aagaagtctt cgtcaaggaa
     1741 caatccgtac ctgccgatct ggaacaagat gagttcgacg tcaaatcaca gcatgtcgtt
     1801 gcgcttgtag gcggcgtacc gatcggcacc ggacggctgc ttcccaatgg gcatatcgga
     1861 cgcatggccg tgctgcgcga ctggcgtcgc atgggcgttg gcgcagccat gttaagggcg
     1921 ctcatgaata tagcgcgtgg cctgggcttg cgccgcgtga tccttaacgc ccagctacac
     1981 gcgttgccgt tttacgttca tcacggcttc cagccggtgg gcgatgagtt catggatgcg
     2041 ggcatcccgc atcgacgaat ggagcgcgat ctctagctgg ctggccgtac tgagctatcc
     2101 gttagactgg gcacaaaacg gaagcgcctg tcctcgagca attcggcccg ctcgccaaag
     2161 caacgctcac ggaaatgaat ttcgccgtcg cccgtaagta gcagcaccgt ggatgcgcgc
     2221 gtgccgtatc ccggtgcagt gatgaatgac gcggacaatc ggcgctccca gtccttgccg
     2281 attccggtat tcggtaacgc atcatccggt gcaatgctgc ggtccgaaag cacagcaagc
     2341 aaactttcaa ctaaaccgtc gggtccctgg ctgctcgcaa tagcaccact caaggctgat
     2401 ttgccgcgct ccacttttgg ccaaggcgta tcgagaagcc cgttgctcaa tccatagaca
     2461 cctgggccaa gcatcatcgt ggccgtttgc tgattggagt aatagtgcag gtgctggtgg
     2521 ttgcccaaca caagattaaa gccggcatag tcgcgcgcgc cgtgtcgaac tgaatccgca
     2581 taggttcccg gtaaagccgg atcgcgcaaa tagtctgcga ccaattcccc acgagatctt
     2641 gcgccaggcc tggcgttgct tccgtcgcga aaattggtaa ccgccgccca gcgcccgtcc
     2701 gttgcgacgc ccatccaggt gccgcctttt tcgagatcgc ggccaccaag aatatgggcc
     2761 gcgtcgggcc agtaatctgc cggcgcagtg ggtcgccgga agaactcgtc gcgattggca
     2821 gcaactacca gtcgatagcg cgcatcggtc tgatatgcga tcaggatgag gcacatcagt
     2881 cttcaaaaca ttccatgtgc cgcgcactgg cgggttccag cacgttttgg aaatgcgcgg
     2941 cttgcacaga caacgccagg cagtgctcga atgcagcggc gtcccgcacg ccttcgtaga
     3001 tctccatcca ggtggtcgcg tcgtcacgtt tccggaccag ccgcccggct ataccgcaac
     3061 gcgtcttcaa ctcggcctgg acgtcacgca ccaacgcttc tgcctgggcg ggccgggcaa
     3121 cgcgatagta gatgtaatag gacagtgcca agcgcttcag tcgggttgga atgcttccga
     3181 ccacactatg gattcaccat ctgctgttcg ggtttgtccc gtatccggtt gcgacactac
     3241 ggcaagaagc gcacatccgg catcaggaac gggcgctgcg ttaaggactg ttccagtttg
     3301 ctgatcggcg ctaaacagtg catcccccga ctgaacctcg ccacgcgtgt ggcccagctt
     3361 gagcttgcgc tttacgtcgc cgagatagcg ggtccgcgcc acgatctcct ggcccggaaa
     3421 gcaacccttg ttaaagctca caccgccaat ctcatccaat ctaaccattt ggggcagaaa
     3481 ctgttctcgg gttgcttgcg tgatccaggg tatgccggcc tcgatctgca accatgccca
     3541 gcattgctca ccggcgggca cggcgcgttc tgccagttcg tcccacagcg cggacatgtc
     3601 tcgcgatgca gctaccacca cgaattgatt acccggcagg cgtatcacgc ttcgatgtgc
     3661 gcgaatgccc accgccatgc gccgggacgg cggcgccgac tgtttctcct ccaataacgt
     3721 aacagcctgt ggaccggcca gacccagcaa ttcctgtccg cgttcatgct cgatgctgac
     3781 ttgggatcgc agcacgtact tacggagtct agtcgccaga tcaccggcaa cttctcgcgg
     3841 cacttgcatc aagtagccgg acgatgcgac gaacagcaaa aaattggcga tcaggcgccc
     3901 tttgggattg caatagccgc cgaactgcgc ctgttcgctc gtcacctcat cgagatcgat
     3961 ggtcagttgc ccttgcagaa atgctttcgc gtctgaacca tggaatcgaa gcagctcgaa
     4021 ctgcctcagg ggagtcacga tcgagttcga gcgagccgcg gcatactgtt cgcgcggctc
     4081 cccgaaatga ggcaccgaac cggcgttttg cttcacgata tcattccagt ccattaacct
     4141 gcccatggct ttcccggtcc tttggaaacg attatataag ggcgtgaaac cgatgcatgt
     4201 accgcgttta tccctgcaga ttaaggcttc ccggcagttg gcgattgcac ttgttctgct
     4261 gcattcggca gcaattgcct gcgtggtgtg gtttctgccc cgagcgtggc tcagcgcagg
     4321 cgcggccatc gctatccttg ccagcctggt attccatctg cgacgggatg ctctgcagct
     4381 ttccggcaac tcggtaaccg cttttacgct caaagaaggc gcgcaatgcg tccttacctt
     4441 caggaatggc ggcacattaa gcggccgcat agaagggttc agtttcactg caccgttgct
     4501 gacggtaatc caggttcgtc ctgccactcg ctggggcggg cgagcagcga ttttgatgcc
     4561 ggacagtgct tcgcaacagg aactgcggca agtcagggtt tggttgcggc acggcgcgca
     4621 actcgacatg gccgattcac gcaattctta gtaccgtgtc ctctgccttc gacagcaagc
     4681 caggatagtc caggctgaaa tggaggcctc gactttcgtg tctgcgcatg gcactgcgca
     4741 cgatcaagtc cgctgtggtg acgaggttgc gcagatcaag caagtcggcg ctgaccttga
     4801 agttcgagta atactcctga atttcgtccg tcaacaggct tattcggtga cgtgcccgtt
     4861 cgaggcgctt gttggtgcga acgatgccga cgtaatccca catgaaacgg cgcagttcat
     4921 gccaattgtg agatatgacg atttcctcat cggcatcggt cacccggctt tcatcccacc
     4981 ggggcaacgc cgtcgatggc aatttggctt gctgcaggat gtgggttgca gcggtttgtc
     5041 caaacacgag gcattcgagt agcgaattgc ttgccagacg gttcgcgccg tgcaggccgg
     5101 tgtgcgcagt ttcacccacc gcgtataagc ccgccaggtc ggttcgcgcg tcgcgatcga
     5161 cttgtatgcc tccgcaggta tagtgcgcgg ctggaaccac aggtatcggc tgccgtgaga
     5221 tatcgattcc gagttccaga catcggctat agatgttcgg aaaatgttcg cgcaggaatt
     5281 ctgccggctt atggctgatg tccagataga cacaatccaa gccgcgcttt ttcatctcaa
     5341 aatcgattgc ccgggcgacc acatcgcgcg gagccagttc cgcgcgttgg tcgtgcgcag
     5401 gcatgaagcg ggttccatcc ggaagcctca ggatgccgcc ttcgccccgc accgcctctg
     5461 agatcaggaa cgatttggca tgcgggtgat aaaggcaggt cgggtgaaac tgaatgaatt
     5521 ccatgttcgc gatgcggcaa ccggcgcgcc agcccatggc gattccgtcg ccggtcgccg
     5581 tgtctggatt ggtcgtatac agatacactt tcccggcccc gcccgttgcc agcacgacct
     5641 gatccgccgc gattgtttca acccgtcccg actggcgatc gaggcaatag acgccaacgc
     5701 agcgcttctc cgccagcccg agcttttctg cggtgatcaa gtcgacggcc atatactgct
     5761 caaggacggt aatattggga tgcgccttga tcttgccctc gagcgtactt tggacagcgt
     5821 ggccagtcgc gtccgccgcg tggattacac gtctgtggct gtgtccgccc tcacgggtga
     5881 ggtgatagcc cagatcgctg gtgtcccggg taaacggcac accctgccca atgagccatt
     5941 cgatagcctg ccggccgtgt tcaacgacgt agcgggtagt tgcggcatca caaagccccg
     6001 cgcccgctat ttccgtatcg cgaatatgat ctccggggct gtcatccttg gccaggactg
     6061 cggcaattcc gccttgcgcc cagttcgaag cgccgtccag cagttcacgc ttggtaaaga
     6121 tacccacctt gcgatggtcg gcaagatgca gcgcaaccgt gaggccggcc agcccactgc
     6181 cgacgatcgc aacgtcgaaa cgcttcatgg gaatctcgtt agttggaagc gcggcagtgt
     6241 accgcaatgc cgcagccgaa aatggtcagg caagccgcaa tttgtctcag tgaactaatg
     6301 cagtcgtgct ctgtctgtgt atgcggatct cgatgcgatt tcccgaatag gtgtcgacgg
     6361 gtatacttgg cgccttttgc gacgcagtgt gatcaataat cggaatattc gggaattgca
     6421 gctagatggg cgaccgtgaa gttgaccaag cgctggtcga gcgggcgcaa cgcggagaca
     6481 agcaggcctt cgagttgctc gtcgcgaagt accagcgcaa gcttggacga ctgctttccc
     6541 ggttcatacg cgatcctgca gaagtggagg acgttgccca ggaagctttc atcaaggctt
     6601 accgagcact gccttcgttc cgcggagaca gtgctttcta cacatggctg tacaggatag
     6661 ggattaacac cgccaaaaat tatctggtgg cgatggggcg gcgcgcgccg acaaccacgg
     6721 agtacgatag cgaggaagcg gaaaatttcg aggacggcga tcagcttcgg gacctcaata
     6781 ccccggaagc ggagctgatg acaaagcaaa tcgcctcgac agtgaaccag accatggaag
     6841 agttgcctga agaactgagg acagcgatta cgttgcgaga gattgaaggc atgagctatg
     6901 aagacatcgc gaacgtaatg aattgtccga tcgggacagt aaggtcgcgc attttcaggg
     6961 cgcgggaaac catcgcggaa cgcctgcggc ccctgttgga caccagtaag gaaaagaggt
     7021 ggtagacgtg gaaaaaatct ctgcattgat ggacggcgaa atcgggggcg cagaagccgc
     7081 tgtccagatc aaacggctta aggaagaatc gcagtttcgc gccgcctggg atacttacca
     7141 tgtgattggg gatacgctgc gcggcgaaaa gctctggctg tcgcaacgct tcaccgagag
     7201 tttcagccag cgtcttgcgc aggaaccgac cgttctggcg ccgcaggtcc gtagcccgct
     7261 acaaacagcc atgcgcaggt tcgcgttgcc tgtagcggct tcggttgggg gcatgatgct
     7321 ggttgcatgg ctcgctttat tcaataatcc gttcgcaccg ccca
//
LOCUS       DATMZQ010000467         4263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3699442, whole genome shotgun sequence.
ACCESSION   DATMZQ010000467 DATMZQ010000000
VERSION     DATMZQ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699442"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..753)
                     /gene="thpR"
                     /locus_tag="VN496_14800"
     CDS             complement(115..753)
                     /gene="thpR"
                     /locus_tag="VN496_14800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXF18273.1"
                     /translation="MRASTSKRRQPRDSAFPGAAKVSRPKRLRCSNEGKSRLAGSKHR
                     VFFALCPDDPVRAQIAQAARRMHRVTQGRRIPDEELHLTLAFIGAVSEERLEHLLKPP
                     SEIFPPAFLLTLDDWGLWTRKGIGWTGPSVVPASLRRLAEKLDSWLRAEGFDLERRTF
                     AAHVTLVRHARPVALPTFGTSIHWHVGDIALMNSAVTADSARYTVLGKYSLL"
     gene            complement(644..1123)
                     /gene="ispF"
                     /locus_tag="VN496_14805"
     CDS             complement(644..1123)
                     /gene="ispF"
                     /locus_tag="VN496_14805"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXF18274.1"
                     /translation="MRIGQGFDVHALVPGRALIIGGVTIAFEKGLQGHSDADVLVHAI
                     CDALLGAAALGDIGRHFPDTDPRYKGVDSRVLLREVARLLRDGGFRIVNIDSTIIAQA
                     PKMAPHIPAMISNLGIDLGIERACINIKAKTTEGLGFPGRGEGIAAEAIALLERGKI"
     gene            complement(1120..1821)
                     /gene="ispD"
                     /locus_tag="VN496_14810"
     CDS             complement(1120..1821)
                     /gene="ispD"
                     /locus_tag="VN496_14810"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXF18275.1"
                     /translation="MPRYFAVIPAAGTGTRMGGAFPKQYLAIAGRPLLYYAVARLCAH
                     AAIENVWVILAPGDSRFQTYDWQPFTGKLEPLYIGGDSRGATVLNALERLSGQSRSDD
                     WVLVHDAARPCLTARMLNRLIDEVGEDPSGGLLAVPVADTLKRASEDSRSLRTESRSG
                     LWQAQTPQMFRYDLLLRALRSCDLVHATDESAAVEALGFRPKLIQSDATNLKVTHEQD
                     FALAELILNHFAGQT"
     gene            2028..>4263
                     /locus_tag="VN496_14815"
     CDS             2028..>4263
                     /locus_tag="VN496_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXF18276.1"
                     /translation="MRLPDFSLMPGARTTLPLLHDSADALALATLLRNSGPMLILTSN
                     AQDAERLRTELAFFAQELRIFLLPDWETLPYDNFSPHQDLISERLATLYQITHKAFDV
                     AIVPVTTALYRLPPASFIAGHTFFLKQGSRLGAESLRKQLVLAGYAHVTQVVSPGEFC
                     FRGGVIDLFPMGSPLPYRIDLFDDEVDSIRTFDVDTQRSIYKVNDVRLLPAREFPSDD
                     AARTAFRVRFREKFEGDPSKSRVYKDVSQGFLPAGIEYYLPLFFDQTATLFDYLPPET
                     QICLHHAVADAVRQFWSDTQSRYNLLRGDPDRPLLPPADLFMREDEFFGCLKPYSRIE
                     LGAPGPITDSASAQATNALPAIAVDRRAANPLTLLHAFCESFDGRVLLVAETLGRRET
                     MLALLHEYQMQPEVCANWAAFEQSTDTLMLTVGPLHAGFVDPDRRIAIVTENELYPAQ
                     VRTRATRERRIAAEGMLRDLSEVKVGDPVVHSQHGIGRYLGLATMDLGEGATEFLTLE
                     YDAGDKLYVPVSQLAVISRYSGAAEESAPLHKLGSGQWEKARRRAAAQVRDTAAELLN
                     LYAQRALRQGHPFSLKAHDYEAFREDFPFEETADQAAAIDAVLADMQSGRPMDRLICG
                     DVGFGKTEVALRAAFVAVADGKQVAVLVPTTLLAEQHFQTFSDRFADWPVKIAELSRF
                     RSAKDTGTALAGLAEGTVDIAIGTHKLLSRGVKFKNLGLAIVDEEHRFGVRQKEQLKA
                     LRAEV"
BASE COUNT          873 a         1276 c         1250 g          864 t
ORIGIN      
        1 cgtcgagcgc acagcccaca ttcacggtgg cactgtacag ctgttgcgcc gctcaggcgg
       61 tggattggaa gcgcgagttg aactgcccat cgtgcctgca aagggcgagg cgaatcaaag
      121 caaagagtat ttgcccagca cagtgtagcg ggcgctgtct gctgtcaccg ccgagttcat
      181 cagcgcaatg tcgcccacat gccaatggat cgatgtgcca aacgtaggca gtgctacggg
      241 cctggcgtgc cggaccagag tgacatgggc tgcaaaagtg cgccgttcca gatcaaatcc
      301 ttcagcacgc agccagctat ccagtttttc ggccaatcga cgcagcgatg ctggaacgac
      361 ggaagggccc gtccagccga tgcccttgcg cgtccacaat ccccaatcgt ccagtgtcaa
      421 cagaaatgca ggcgggaaga tttccgaagg aggcttaagc aaatgctcca ggcgctcctc
      481 gctaaccgcg ccgatgaacg ccagggtaag gtgaagctct tcgtccggta tgcgacggcc
      541 ctgtgtcact cggtgcatgc ggcgggccgc ttgcgcgatc tgagcgcgca cgggatcgtc
      601 cggacacaga gcgaaaaaga ccctgtgctt gcttcccgcc aacctagatt ttccctcgtt
      661 cgagcagcgc aatcgcttcg gccgcgatac cttcgccgcg cccgggaaag ccgagtccct
      721 cggttgtctt cgctttgatg ttgatgcacg cacgctctat gccgaggtca atgccaaggt
      781 tagaaatcat ggccggaata tgcggcgcca tctttggcgc ctgcgcgatg atggtcgagt
      841 cgatattgac gatgcggaag ccgccgtccc gtagcagcct cgccacttcg cgcagcaaca
      901 ccctgctgtc gacacccttg tatcgcggat cggtatctgg aaagtgacgg ccgatgtcgc
      961 caagcgctgc tgccccgagc aaggcatcgc agatcgcgtg cacgaggaca tccgcgtcgg
     1021 aatgaccttg caaacctttc tcaaacgcga tggtgacgcc tccgataatc agtgctcggc
     1081 ccgggaccag cgcgtgcaca tcgaatccct gaccaattct catgtttgac ccgcgaagtg
     1141 gttcaatatc agttctgcca atgcgaaatc ctgctcatgt gtaaccttca ggttggtcgc
     1201 gtcggactgt atgagctttg gacggaaacc aagggcttct acagcggcgg attcatccgt
     1261 tgcgtgaacc aggtcgcagg accgtagtgc ccgcaggagc agatcatagc gaaacatctg
     1321 cggggtctgg gcctgccaca atccgctcct tgattcggtg cgaagggaac gggaatcttc
     1381 actggctcgc ttaagcgtat cggctaccgg cacggccaga agcccgccgg atggatcttc
     1441 gccaacttcg tcgatcaacc ggttcagcat gcgcgcagtc aggcacgggc gtgccgcatc
     1501 atgcaccaat acccagtcat cagaacgcga ctgcccggaa agtcgctcaa gggcattgag
     1561 caccgtcgcg ccacgggaat cgcctccgat gtacagtggc tccagtttgc cggtgaacgg
     1621 ctgccagtca tacgtctgga atcgactgtc gcccggcgcc agtatgaccc aaacgttttc
     1681 aatcgcggca tgtgcgcata ggcgcgcaac ggcgtaatac agaagcgggc gtcctgcgat
     1741 agcaagatat tgcttgggaa aggctccgcc cattctagtg ccggtaccgg ctgcgggaat
     1801 aacggcgaaa tagcgcggca taagagtcag gtgaggggcg cgaattcttg gctatcggaa
     1861 gagtccctgc cgcgccacgg cgggccattg ccggcgatct taattcatct tcgcgcgctt
     1921 ggaaaacaat atcagcgcag cctccggcca ggtcgcctca tacgcgtgtt cggaaggaag
     1981 cgagatcggc ttccactata attgcgactc atatccaggc cgtaacaatg cgactgcccg
     2041 atttctccct gatgccggga gcccgcacca cccttccact gctgcacgat tccgccgatg
     2101 cgctggcgct tgccacgctt ctgcgtaact cggggccgat gctgattctg acgtcgaatg
     2161 cccaagacgc ggagcgatta cgtacggaac tcgcattctt tgcgcaggag ttgcgcattt
     2221 tcctgttgcc ggactgggaa accctgccct acgacaattt ctcgccgcac caggatctca
     2281 tttcggagcg gcttgccacg ctctaccaga tcacacacaa agccttcgac gtagcgatcg
     2341 tcccggttac cacggctctg taccgattgc cgccggcaag ttttattgca ggacacacgt
     2401 ttttcttgaa gcaggggtcc aggcttgggg cagaatccct gcgaaagcaa ctagtcctgg
     2461 ccggatatgc gcatgtcacg caagtggttt cgccaggcga attctgcttt cggggcggtg
     2521 tcatcgacct gtttccgatg ggcagtccgt taccttaccg gatcgacctg ttcgatgacg
     2581 aagtggatag cattcgaacg ttcgatgtcg atacccagcg cagcatctat aaagtcaacg
     2641 acgtgcgttt gctgccggcg cgtgagtttc cgtcggacga cgctgcccga acggcattcc
     2701 gtgtacgctt tcgagaaaaa ttcgagggcg acccctccaa gagccgcgtc tacaaggatg
     2761 tcagccaggg gtttcttccc gcaggcatcg agtattacct gccacttttc ttcgatcaaa
     2821 ccgcaacgct gttcgactat ttgcctccgg aaacccagat ttgtttgcat catgcagttg
     2881 cagacgcggt tcggcagttc tggagcgaca cgcaatcgcg ctacaacctg ttgcggggcg
     2941 atccggaccg gccgttgctg cccccggccg atcttttcat gcgcgaggac gaatttttcg
     3001 gctgcctgaa accgtattcc cgcatcgagc ttggcgcacc aggacccata accgatagcg
     3061 cgtcggcaca agcaacgaac gcgctgcctg cgatcgcggt cgatcgccgg gcggccaatc
     3121 cactcacgct tttgcacgcg ttttgcgaaa gcttcgatgg ccgtgtgctg ctagtcgccg
     3181 agacgcttgg ccgacgcgaa acaatgcttg cccttttaca tgagtatcag atgcaaccgg
     3241 aggtctgtgc caactgggcg gcgttcgagc aatcaacgga tacgctgatg ctgacagtcg
     3301 gcccgctgca tgctggattc gtcgatcctg acagacgcat cgcgattgtt accgagaacg
     3361 agctgtaccc agcgcaggtc cgcacgcgcg cgacacgtga gcgccggatc gcggcggagg
     3421 gcatgctgcg ggacctttcc gaggttaagg ttggcgatcc ggtcgtgcat tcgcaacatg
     3481 gcatcggccg ctatctgggc ctggcaacca tggacctcgg agaaggcgcg acggaatttt
     3541 tgaccctgga atacgatgcc ggcgacaagc tgtatgttcc agtttcgcaa ttagccgtta
     3601 tcagccgtta cagcggcgca gccgaggaat ctgcaccgtt gcacaagctt ggcagcggtc
     3661 aatgggagaa agcccgccga cgcgccgcag cgcaggtcag ggatacggct gccgaactgc
     3721 tcaacctgta tgcgcagcgt gcgttacgtc aaggtcaccc cttctcactt aaagcgcacg
     3781 attacgaagc gttccgggaa gacttcccgt tcgaagagac ggccgatcag gcggcggcaa
     3841 tcgacgccgt acttgcagac atgcaaagcg ggcgcccgat ggaccggttg atttgcggag
     3901 acgtcggttt tggcaaaact gaagtggcct tgcgcgcagc tttcgtcgct gttgccgatg
     3961 gcaagcaggt agcggtcctc gtgccgacaa ccttgctcgc cgaacagcat ttccagactt
     4021 tctcggatcg cttcgcggac tggcccgtca agattgcgga gctttcccgg tttcgctccg
     4081 cgaaggacac gggcactgca ttggctggcc tggcggaggg cacagtggac atcgcgatcg
     4141 gcacgcataa actgctctca cgcggcgtga agttcaagaa cctgggcttg gccattgtcg
     4201 acgaagagca ccgtttcggc gtgcgccaga aagaacaact caaggcattg cgcgccgagg
     4261 tgg
//
LOCUS       DATMZQ010000468        13719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4390331, whole genome shotgun sequence.
ACCESSION   DATMZQ010000468 DATMZQ010000000
VERSION     DATMZQ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4390331"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VN496_14820"
     CDS             <1..676
                     /locus_tag="VN496_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXF18277.1"
                     /translation="LTVVDPKIVPLSAYLGVCGMPGATAWMGLLEYCAPKAGETVLVS
                     AATGAVGSVVGQLAKLQGCRAVGIAGGAKKCEYAVKELGFDACVDYRAGKLIDDLKAA
                     CPKGVDNYFENVGGEVMDAAFRVLNPFSRVALCGMISDYNATEAYGTKMMRALLVNRV
                     KLQGFIVFDRQDLYARAVAQLVNWVSQGKIKYHESIADGLRNAPKAFIGLLKGQNLGK
                     QLVKLV"
     gene            complement(731..1246)
                     /locus_tag="VN496_14825"
     CDS             complement(731..1246)
                     /locus_tag="VN496_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HXF18278.1"
                     /translation="MKAFQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAKMSEAI
                     LAASACRTSITETVQSASSLPIAGLWGCESSSTTAQVSKYVTSIKTSSKGEIRVQVTG
                     INATNVDGNAIILQPSNAPTTAVAPAVGDAVASWLCGPDPANAKDISKFLPGSCRAAI
                     TVYSGTFASST"
     gene            complement(1595..2446)
                     /locus_tag="VN496_14830"
     CDS             complement(1595..2446)
                     /locus_tag="VN496_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18279.1"
                     /translation="MPSLLKSLRIVLAVIASLVLVSRAQAQSQSGNNDGDVINWWYSA
                     TFGTGVYTSGSRSVAVLQVPFAHALKNVDEDNYGLKFKVSTTLGFYDYSVGSAINGNI
                     PDRISTFSVLPGFEWELPMSQRWVLRPFADAGFGQELTGHQSASIFDFGVKSRFLLAQ
                     NRGVEFVLVNALTSAGYRQSGESTNTFGYFATGLDITIPTDRSAFGRPLYFGFTPVYF
                     YYFNKFSLGEFNDRQNRIREEFELAMSVVTRKPWSLSFFDFDRVGLAIRSSGDVVGVS
                     LFTSLPF"
     gene            complement(2465..3736)
                     /locus_tag="VN496_14835"
     CDS             complement(2465..3736)
                     /locus_tag="VN496_14835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF18280.1"
                     /translation="MAATLFNNVSILDCTGAQPFNGCVLVEGNLITAVVPHAVSLTVP
                     GATAVDGAGRTLMPGLVEAHSHLTFTDVAQSVELGFIPPEEHMLKTAANARCMLDAGF
                     TSCFSAAAAKPRLDIVLRNAIDSGDLPGPRTLAASPEMTVAAGLGDVRLLHMYRENFA
                     VVCNGPEEFRRFSREMVREGVDTLKINVSGDAGTPASPADKTVMNEAEVAAVCDVAQA
                     HGKRVAAHARSAASVSMCLRHGVEVIYHATLLDDVARSQIEAQRERVFVAPVLGNLYT
                     TLHEAAPWGISREIAEERGIAFELEQGIHNMKLLHLQGVRVLIGGDYGFAWNPIGTDA
                     REISHFVDMLEFSPMDAILAATRLGGQIMGMGDRLGQIRPGYLADLLLVNGDPIKNVA
                     ILRQRESIEAIMRDGIFWKTPATGYRDYTTV"
     gene            complement(3751..4500)
                     /locus_tag="VN496_14840"
     CDS             complement(3751..4500)
                     /locus_tag="VN496_14840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18281.1"
                     /translation="MSKLKGKVAWITGAGSGIGLAGAQALAEAGATVVLSGRRKEVLA
                     PEAEKIRIKGGAAEVEALDVSEAKAVKKVADGILSRHGRIDILVNSAGLNTPNRYWKN
                     QTVDGWREVIGINLDGSFYTIHAVLPAMRARKDGLIINVSSWAGVFHPKLTGAAYNGS
                     KHAVTAMTETINMEECANGIRACAVCPAEVATPIMDRRPVPPSAADRAKMLQPEDLGN
                     TIRFIAELPAHACINQLIISPTWNRMYIENL"
     gene            complement(4516..5493)
                     /locus_tag="VN496_14845"
     CDS             complement(4516..5493)
                     /locus_tag="VN496_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HXF18282.1"
                     /translation="MALDPQIEIILGLARQANLPEIWQITPDQGREQYLTRVNKLKFT
                     EPIFRTEDRRIAGPGSDLAIRIFTPRELKTSEKLPVLMWFHGGGFVIGSIDTHDSVCR
                     MLANRADCLVVAVDYRLAPESKFPAAVEDCEAALKWTALHAAEFGGDATCIAVGGDSA
                     GANLATVIAILARDAAHPKLAFQLLIYPCTAPEPETASHHKFKEGYVLTRKSITWFYR
                     QYLRSGRDVKDFRYAPLVHDDLAGLPPCLIIVAGYDPLRDEGIEYAKKLIEAENRVRL
                     SNYEGMVHGFYLMGGAVDAARQAVDESAQALRQAFDNTRGIDRNLAKTA"
     gene            complement(5502..5903)
                     /locus_tag="VN496_14850"
     CDS             complement(5502..5903)
                     /locus_tag="VN496_14850"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXF18283.1"
                     /translation="MATQKAGFHIHVPFVEHLGVRIVHMEPGIVRLRLDPRPELENSW
                     GSMHGGVLMTLLDVALASAGRSLDEECNGALTVEMKVNFIAAATGAMLAEGRAQWAGR
                     SLLFSEGELRSEDGTLLAKATGTFKLLYPKE"
     gene            complement(5916..6692)
                     /locus_tag="VN496_14855"
     CDS             complement(5916..6692)
                     /locus_tag="VN496_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18284.1"
                     /translation="MKVRELFDLSGKVALITGGSRGLGLQMAEALGEMGARVIVTARK
                     AAELEEAKAHLKATGIDALTIVNDLQNTDSVKPMIEQVLKSHGHIDVLLNNAGAAWGA
                     PAEDHPLEAWYKVINLNLTAVFLMCQSVGKLSMIPRKYGRIINLASIAGLVGTDPRMM
                     PTIAYNASKGGVVNLTRSLAVEWAHHGVTVNAIAPGVFPSKMSQGMIDKAEQVILEIT
                     PMKRLGNEDDLKGLAVLLASDASAYITGQIIAVDGGLVAM"
     gene            complement(6749..8554)
                     /locus_tag="VN496_14860"
     CDS             complement(6749..8554)
                     /locus_tag="VN496_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXF18285.1"
                     /translation="MSKLLNRRDVEFMLYELLDVESLTQRQRFAEHTRETFDAALDTA
                     AQIAEEKFAPHNRKADLNEPRFENGRVNIIPEVAEALQAFRKAGFMAAAQDFEVGGMQ
                     LPWTVAQACFAWFNAANISTAGYPFLTIANANLIRSFGSPAQKQMYLPALLSGRFFGT
                     MCLSEPQAGSSLSDIRTTAHLNQDGSYRMVGNKMWISAGEHDLSENIVHLVLARIHGA
                     PAGVKGISLFIVPKYRVNDDGTLGKRNDIALAGLNHKMGFRGTTNCVLNFGEQGGAYA
                     EIVGKPNEGLAYMFQMMNEARIGVGMGATVLGYTGYLHALAYARERPQGRLPGGKSAQ
                     LPPVKIIQHADVRRMLLAQKAYVEGALALCLYCSFLVDESRTAEDAKSRRNAELLLDI
                     LTPIAKAWPSQYCLEANSLAIQVHGGYGYTREYPVEQFYRDNRLNPIHEGTNGIQAID
                     LLGRKAGMHDGAAVQSLARQISSAVNEALACESAELQRCGALLNAALDRVMSVTRDLL
                     CVIARGEVDSGLANASVYLDLVGHVVIAWMWMKQALVAVKRVGTADETSRNFYLGKLQ
                     ACSFFFRWELPKTNQWADLLDSIDPTCLDMNSEWF"
     gene            complement(8592..9767)
                     /locus_tag="VN496_14865"
     CDS             complement(8592..9767)
                     /locus_tag="VN496_14865"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXF18286.1"
                     /translation="MDAVIVSTARTPLCKSWKGALNMTHGATMGGHVVKAALERAKLD
                     PAEVEDVIMGCANPEGATGQNIARQIALRAGMPVTVSGMTVNRFCSSGLQTVALAAQR
                     ILAGEANIFVAGGVESISCVQNEANKHMLRENWLEQHKPEIYWPMLATAEMVSKRYKI
                     GREAQDRYGVQSQQRAAAARAAGKTKDEIVPISTTMKVIDKNTGQESTRQVTASEDEG
                     IRPDTTYEGVSQIKPAIEGGVIAAGNASQFSDGASAQVVMSDRVAAGRGLKPMGIFRG
                     FAVAGCEPDEMGIGPVYAIPKLLKQTGKKIGDIGLWELNEAFAVQVIYCRDKLGIPDD
                     RLNVNGGSIAVGHPYGVTGSRLIGHALIEGKRRGATYVVVTMCIGGGMGAAGLFEVV"
     gene            complement(9841..11937)
                     /locus_tag="VN496_14870"
     CDS             complement(9841..11937)
                     /locus_tag="VN496_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXF18287.1"
                     /translation="MSLVDYQKQGSIGVITLNNPPVNALSVNKGVVQGILDAFKEGDH
                     DHHVRAFVIIGGGRNFSGGADISEFGKGYDPGKAMLPDVLGYMDTVSKPIVAAISGPT
                     MGGGLELALACHYRVALTGAQIALPEVKLGILPGAGGTQRAPRLMGVAKALEFMVSGD
                     PISSEMAKDTGLVDEVVTGDLRQAAIAYANRLLKDNRGVRRTSQLSPALDQPAESFFQ
                     AARERIAKNWRGYPAPLAIAACVQAAVTQPFAKGISFERQQFEKLVKSDESRALRHLF
                     FAERQVSKIPDVPEDTPTHDIKSAALIGAGTMGGGIAMNFANAGIAVKMLELNQEALD
                     KGLGTVRKNYAATVSKGRLTQEAMDKRMSLFTGVTRYEDIKNADIVIEAVFEDMAVKK
                     QVFQKLDQVCKPGAILATNTSTLDVNEIAAMTSRPQDVLGLHFFSPANVMKLLEVVRA
                     RKTSKEVLASAMKLSKAIKKVGVVAGVCDGFIGNRMLHGYFREAGFLLEEGALPQQVD
                     KVIEDFGFAMGPFRVGDLAGLDVGWYIRKRQAATRPAHLRYSKVADQICELGRFGQKT
                     GAGWYRYEAGNRNPIPDPIVEELIVEASREAGIERRQITDQEILERCIFALVNEGAEI
                     LEEGIALRASDIDIVYIYGYGFPRYRGGPMFYADTIGLDKVLASVQAYHSAHGEYWKP
                     AVLLERLAAEGKKFNQ"
     gene            complement(12052..12747)
                     /locus_tag="VN496_14875"
     CDS             complement(12052..12747)
                     /locus_tag="VN496_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18288.1"
                     /translation="MLEVNNLHAYYGKSHILHGVHLKVGAGEIVSLLGRNGVGRSTTI
                     KSIMGQVNSTGSVKFKGEEINGQKTYMVARRGLGYVPENREIFPSLTVKQNLLLGMKR
                     ARQRGRWSVDDMYALFPRLKERAEAPAGVMSGGEQQMLTLCRTLMGDPDLVMIDEPTE
                     GLAPKIVEQVARLFEEIKNRGVSILLVEQKLTIAMKISQRLYVMGHGKIVFEGTPDGL
                     RANEAVRKEWLEV"
     gene            complement(12786..13538)
                     /locus_tag="VN496_14880"
     CDS             complement(12786..13538)
                     /locus_tag="VN496_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18289.1"
                     /translation="MPVAALRLHDVRKSFGRTSIINGVNLSIEPGERHAIIGPNGAGK
                     STLFNLISGRFGVTSGRIDLHGEDITGLEPEIINRKGLARSFQITNIFPRMSVYENVR
                     CAVLWSQGYKYSFWHHVDALEAVRERTSDIVERIGMAKRRDRLAGVLTYAEQRALEIG
                     ITIAGGANVVLLDEPTAGMSRSETDEAVELIRKVTEGRTLIMVEHDMSVVFNLADRIS
                     VLVYGEVIASGTPPEIRGNSAVQEAYLGTEQH"
BASE COUNT         2918 a         4045 c         3717 g         3039 t
ORIGIN      
        1 cctgaccgtg gtggatccaa agatcgtgcc gttgtccgca tatctgggcg tgtgcggcat
       61 gcccggcgca accgcatgga tgggcttgct ggaatactgc gcaccaaaag ccggagaaac
      121 ggttctggtg tccgcggcta ctggagcggt cggcagcgtc gtcggccagc tcgcgaaact
      181 gcaaggttgc cgtgcggtgg gcattgccgg cggtgcgaaa aaatgtgaat acgcagtgaa
      241 ggaactgggg ttcgacgcgt gcgtcgatta cagggcaggc aaattgatcg acgatctgaa
      301 agctgcgtgc ccgaaaggcg tggacaatta cttcgagaat gtcggcgggg aagtcatgga
      361 cgccgcattc cgggtgctga atcctttttc ccgggtcgcg ctatgcggca tgatttcaga
      421 ctacaacgcc acggaagcct atggcacgaa gatgatgcga gcgctgctgg taaaccgggt
      481 caagcttcaa gggttcatcg tgttcgaccg acaggatctt tacgccaggg cagtggctca
      541 gctggtgaat tgggtttcac agggcaagat caaatatcac gaaagcatcg ccgacggact
      601 acgaaatgct ccgaaggctt ttatcggatt gctcaaagga caaaatctgg gcaaacagct
      661 tgtcaaactt gtgtagccaa aaaaaagaag ggccaacagg cccttcttta aagctacacg
      721 agcatttcga tcaggtgctg gaagcaaagg taccgctata gaccgtaatc gcggcacggc
      781 aggagcccgg caagaacttc gaaatatctt tcgcgttcgc cggatccgga ccgcacaacc
      841 agctcgccac ggcatcacca actgccggag caaccgcggt ggtgggggca ttggagggct
      901 gcaggatgat ggcattgccg tccacgttgg tggcattgat gccggtgact tgcacgcgga
      961 tttcaccttt ggaactggtc ttgattgacg tcacgtactt cgagacctga gcggttgtcg
     1021 agctagactc gcaaccccac agtccagcga taggcagcga gctcgcggat tggacggttt
     1081 ccgtgatact ggtacggcat gccgacgcgg ccagaatggc ttccgacatc ttggcgcggg
     1141 cggtgtagtc ctggtatgcc ggcaacgcga ccgctgccag aataccgatg atcgcaacga
     1201 cgatcatcag ttcgataagc gtaaagcctt tctgaaatgc tttcattggc aacttccttg
     1261 tctgtgatta agtgaaacgc accacattgg gagcgaatgg cgagttcatg cagtcatagc
     1321 gcaatagcca tgcccaatgg ctcaatgcct caaaaaaaaa gtgcatttgt tgtctgccgg
     1381 atcagaacag cattattaac agcatcttac tgcgccgccc ggcttacggc gcagccgagc
     1441 tcgcctggcc cacggaaacc gcacggcgca tttcggaaaa gcaataaggg cacattcgcg
     1501 gctctttaaa cccattagga gcactgcttt cagcagacac catgctgtcg gcatgagtgc
     1561 gctctctctg agcccgcgga gaaccgtatt cgcctcagaa cggcagcgaa gtaaacaggc
     1621 tcacgccgac cacgtctccg ctgctgcgta tcgcgagccc gacgcgatca aaatcgaaaa
     1681 acgacagtga ccagggcttg cgggttacaa cgctcatggc gagttcaaac tcttcccgta
     1741 tccggttctg ccgatcgtta aactcaccca ggctgaactt gttgaagtaa tagaagtaaa
     1801 cgggcgtgaa accgaagtac agtggccgcc cgaacgcgct gcggtcggtc ggaatggtaa
     1861 tgtcgaggcc tgtcgcaaaa tagccgaatg tattggtcga ctcaccgctc tggcgatagc
     1921 cggccgacgt caacgcattc accaatacga attcaactcc cctgttttgc gcgagcagaa
     1981 agcggctttt caccccaaaa tcgaagatcg aagccgattg gtgacccgtc aattcctgcc
     2041 cgaagcccgc atcggcaaat ggccgcagta cccagcgctg gctcatcggt aattcccatt
     2101 cgaaaccggg caaaacgcta aaagtgctta tccggtctgg aatattgccg ttgatggccg
     2161 aaccgacgct gtagtcgtag aaacccaagg tcgtcgacac tttgaactta agtccataat
     2221 tgtcctcgtc tacgttcttc aacgcatgtg caaacggaac ctgcaggaca gccactgatc
     2281 gactgcccga agtatagaca ccagtgccaa aagtggcgga gtaccaccag ttgataacgt
     2341 ctccgtcatt gttgccagat tgggattgcg cctgcgcgcg ggaaaccagc accaaggacg
     2401 cgatcacggc caacacaatt cgcaatgact taagcaacga aggcatcccg gaaccgagct
     2461 acgcctatac cgtggtgtaa tcccgatatc cagtggcggg tgttttccag aaaatcccgt
     2521 ctcgcatgat ggcttcgatg gattcccgct gccgcaggat ggcgacattt ttgataggat
     2581 ccccgttgac cagcaacaga tccgcaaggt agccggggcg aatctgcccg agccggtcgc
     2641 ccatacccat gatctggccg cctaggcgcg tggccgccaa gatcgcatcc attggcgaaa
     2701 attccagcat gtcaacaaaa tgactgattt cgcgggcatc cgttccaatg ggattccatg
     2761 caaaaccata gtcgccgcca atcagcacgc gcactccctg caggtgcaac agtttcatgt
     2821 tgtgaatgcc ctgttcaagc tcgaatgcga tgccgcgttc ctcggcgatc tcccggctga
     2881 ttccccatgg agcggcctca tgcaatgtgg tgtaaagatt cccaagaacc ggtgcgacga
     2941 acacccgctc gcgttgcgct tcaatctgac tacgggctac atcgtcgagc aatgtggcgt
     3001 gatagatgac ctctacaccg tgacgcaagc acatgctgac ggacgcagca ctgcgcgcat
     3061 gggcagccac gcgcttaccg tgcgcctgtg ccacgtcgca taccgccgcc acttcggctt
     3121 cgttcataac ggtcttatcg gccggtgaag caggtgtgcc tgcgtcaccg gaaacattga
     3181 tcttcagcgt atcaacgcct tcacgcacca tctcgcgtga aaaacggcgg aattcttccg
     3241 gaccattgca aaccacggcg aagttttcgc gatacatatg caagagccga acatcgccca
     3301 gtccagccgc gaccgtcatt tccggactgg ccgccagcgt tctgggcccc ggtaaatcgc
     3361 cactgtcgat ggcattgcgc aatacgatgt ccagccttgg tttggcggcc gctgcactaa
     3421 agcaactggt gaatcctgca tccagcatgc accgcgcatt ggccgcggtc ttcagcatgt
     3481 gttcttcggg aggaatgaaa ccaagttcga ccgattgggc tacgtcggta aaggtgaggt
     3541 gggaatgagc ctcgacaagg ccaggcatca atgtacgacc tgctccgtcc acggccgtcg
     3601 cgccaggcac ggtaagcgaa acggcgtgcg gcacgacggc cgtaatcaga ttcccttcaa
     3661 cgagaacgca tccgttgaaa ggctgtgcgc ccgtgcagtc caggatgctg acattgttga
     3721 acagcgtcgc tgccatgaag cacagtcaca tcacaggttc tcgatgtaca tccgattcca
     3781 ggtcggactg atgatcaatt gattgataca ggcgtgcgcg ggcaattcgg cgatgaagcg
     3841 gatggtattg cccagatctt ccggctgaag catttttgcg cgatcagccg cactcggtgg
     3901 gaccgggcgc cggtccatga tcggcgtcgc cacttcagcg gggcaaacgg cgcaggcgcg
     3961 aatgccgttt gcgcactctt ccatgttgat cgtctcagtc atcgctgtga cggcatgctt
     4021 gctgccgttg taagcagcac cggtcaattt gggatggaaa acgccggccc aggaagacac
     4081 gttgatgatc agaccatctt tcctcgcgcg cattgccggc agtaccgcgt gaatggtgta
     4141 aaaagagcca tccaggttaa tcccgatcac ctctctccag ccatccacgg tctggttctt
     4201 ccagtaacgg ttaggtgtgt taagccccgc actattcacc aatatatcga tgcgtccatg
     4261 gcgggagaga atgccatcgg cgactttctt cacggccttc gcttcggaca cgtccagtgc
     4321 ttcgacttcc gctgcgcctc ccttgatgcg aattttttcc gcttccggcg ccagcacttc
     4381 cttgcgccga ccggataaca caacggtggc accggcttcg gcgagcgctt gtgcaccggc
     4441 cagaccaatg ccgcttccgg cgccagtgat ccatgcgact ttccccttga gtttgctcat
     4501 gtcccgctcc tgtcgttacg ccgttttcgc cagattgcga tcgatcccgc gcgtgttgtc
     4561 gaacgcctgc cgcaacgcct gcgccgattc atccactgcc tgtcgtgctg catctaccgc
     4621 accacccatc aaatagaaac cgtgaaccat tccttcgtaa ttggaaaggc gcacgcggtt
     4681 ttcagcttca atcagctttt tggcgtattc gatgccttca tcgcgcagcg gatcatatcc
     4741 ggcaacaatg atcaggcacg gcggcagccc agcaagatcg tcgtgcacca ggggcgcata
     4801 acgaaaatct tttacgtccc ggccactgcg caaatactgg cggtagaacc acgtaatcga
     4861 tttgcgggta aggacatagc cttccttgaa tttgtgatgc gaggctgttt ccggctccgg
     4921 tgcggtacag ggataaatca gcaactgaaa cgcaagtttc ggatgagcag cgtcacgcgc
     4981 gaggatcgca atcactgtgg ccaagtttgc gcccgcactg tcgccaccga ctgcaatgca
     5041 ggtcgcatcc ccgccaaatt cggcagcgtg cagtgcggtc catttcagtg ccgcctcgca
     5101 gtcttctact gcggccggga atttcgattc gggcgccaga cgatagtcga ctgcgacgac
     5161 gaggcagtcg gcgcggttgg cgagcatgcg gcaaaccgaa tcatgggtat cgattgaacc
     5221 gatgacaaaa ccgcctccgt ggaaccacat cagaacagga agcttttcgg atgttttcag
     5281 ttcccgcggt gtgaagatac gaatagcgag gtcactgccg ggccccgcaa tacgccggtc
     5341 ctcggtgcgg aagattggtt ccgtgaattt gagcttattg acgcgggtga gatactgttc
     5401 acgtccctgg tcgggggtga tttgccagat ctcgggcagg ttcgcttgcc tggccagtcc
     5461 gaggattatt tcgatttgcg gatcgagtgc catgtagctc tctattcttt tggatataac
     5521 agtttgaatg tcccggtcgc cttggcaagc aaggtaccgt cctcactgcg caattcgcct
     5581 tccgaaaaca gcagcgaccg gcccgcccac tgggcgcggc cttcggccag catggcgccc
     5641 gttgctgcgg cgataaaatt tactttcatt tccacggtca gggcgccatt gcattcctcg
     5701 tccaacgaac gccccgcact agccagagct acgtcaagca gcgtcatcaa caccccgccg
     5761 tgcatggaac cccaactgtt ctccaattca gggcgtggat cgagacgaag cctgactatg
     5821 ccaggttcca tgtgcacaat gcgaacaccc agatgctcga caaacggcac gtggatgtga
     5881 aagccggctt tttgcgttgc catcgaggtc attagctaca ttgcgacaag gccaccatcg
     5941 accgcaatga tctgcccggt aatataggcg gatgcgtcag atgccaatag cactgcaagg
     6001 cccttcaggt cgtcctcgtt accaaggcgc ttcatcggtg ttatttccag aatcacctgt
     6061 tcggccttat cgatcatccc ttgcgacatt tttgaaggaa acacacccgg tgcaatggca
     6121 ttgaccgtga cgccgtgatg ggcccattcc acggccagcg agcgcgtaag atttacgacc
     6181 ccgcctttgc tggcgttata ggcaatggtc ggcatcatgc gcgggtccgt acccaccagg
     6241 cccgcaattg aagctagatt aatgatgcga ccgtacttgc gcggaatcat gctcagtttg
     6301 ccgacgctct ggcacatcaa aaatacagcc gtaaggttga ggttgatgac cttgtaccac
     6361 gcctccagcg gatggtcctc ggccggcgca ccccatgctg ctccggcatt attcagcagc
     6421 acatcgatgt gtccgtgact tttcaatacc tgctcaatca tcggcttcac tgaatcggta
     6481 ttctgcagat cgttcacgat ggtcagtgca tcgatcccgg tggccttgag atgcgctttt
     6541 gcttcctcca gttcagccgc tttgcgcgcg gtaacgatga cccttgcccc catttcaccg
     6601 agggcttcgg ccatctgcaa tccgagtccg cgagagccgc cggtgatcaa agctactttt
     6661 ccggacaggt cgaacagttc ccttaccttc atgtgccgct ccgtatttgt cagttcgttc
     6721 cgcagccgcg caaattgcgc ggtatggcct agaaccattc actattcata tcgaggcagg
     6781 tcggatcgat gctatcgagc aaatcggccc attgattggt ttttggcaat tcccagcgga
     6841 agaagaagga acacgcttgc agtttgccaa ggtagaaatt ccggctggtc tcatcagctg
     6901 tgccaacccg cttcaccgcc acaagagctt gcttcatcca catccaggcg atgaccacgt
     6961 gtccgaccaa atccagatag accgaagcat tggctaatcc cgagtccact tctccacgcg
     7021 cgatcacgca gagcaggtcc cgcgttaccg acatgacgcg atccagagca gcattcagca
     7081 gagcgccgca gcgctgcaat tcggcggatt cgcaggcaag cgcctcgttc accgcgctgc
     7141 tgatttgccg cgcgagcgat tggaccgcag ctccgtcatg cataccggct ttgcgtccca
     7201 ggaggtcaat tgcctggatc ccgtttgtac cttcgtgaat tggattgagg cgattatcgc
     7261 ggtagaactg ttcgaccgga tactcacgcg tgtagccgta gccgccatgc acctgaatcg
     7321 ccaggctgtt ggcttccagg cagtattgcg agggccacgc tttcgcgatt ggcgtcagaa
     7381 tgtccagcag cagttctgcg ttgcgccggc tctttgcatc ttcagcggtg cggctttcat
     7441 cgaccaggaa ggagcagtac aggcaaagcg ccaaggctcc ttcaacatac gccttttgcg
     7501 cgagcagcat gcgcctgaca tcggcgtgct gaatgatttt aaccggtggc aattgtgcgc
     7561 ttttaccgcc tggcagcctg ccttgcggcc gttcgcgcgc ataagcgagc gcatgtagat
     7621 agccggtata gcccaggaca gtcgctccca tgccgactcc gatgcgcgcc tcgttcatca
     7681 tctggaacat gtaagcaagg ccttcattcg gcttgccaac aatttccgcg tacgcgccgc
     7741 cttgctctcc gaaattgaga acgcaattgg ttgtgccgcg gaatcccatc ttgtgattga
     7801 gcccggcgag cgcgatatcg ttgcgcttgc ccagagtacc gtcatcattg acccggtact
     7861 tcggcacgat gaatagcgag atgcctttga cgcccgctgg cgcaccgtgg atgcgcgcca
     7921 acacgagatg cacgatgttt tccgatagat catgttcgcc cgcggaaatc cacatcttgt
     7981 ttccgaccat gcggtagctg ccatcctggt taagatgcgc cgtcgtgcgg atatcggaca
     8041 acgaagaacc ggcttgcggt tcggacaggc acattgtccc aaagaaccgg ccggacaata
     8101 gcgcaggcag atacatttgt ttttgcgccg gtgacccaaa gctgcggatc aggttggcgt
     8161 tggcaatagt caggaaagga taacctgcgg tagagatatt ggcggcgttg aaccaggcaa
     8221 agcaggcttg cgcgaccgtc cagggcagtt gcatgccgcc cacttcgaaa tcctgcgccg
     8281 ctgccatgaa tcccgctttc cgaaaagcct gcaacgcttc tgcgacctcc ggaatgatgt
     8341 tcactcgccc gttttcaaac cgaggctcat tcagatcggc cttgcggttg tgcggtgcga
     8401 atttttcttc cgcaatttgt gcagcagtat cgagcgccgc atcgaacgtt tcgcgcgtat
     8461 gctcggcgaa gcgttgccgc tgtgtcagcg actcaacatc gagcaattca taaagcatga
     8521 actccacatc gcggcggttc agcagtttag acacccaccc acctcaccgt cgaaatgaag
     8581 ccagctgtca attaaactac ttcgaacaaa ccagcagcac ccatgcctcc gccgatgcac
     8641 atggttacaa cgacgtacgt tgcgccgcgg cgtttgccct cgatcaatgc gtgtccgatg
     8701 aggcggctac cagtcacccc atacggatgg cccactgcaa tcgaaccgcc gttgacgttc
     8761 agccggtcat cggggatacc aagcttgtcg cgacagtaaa tcacttgcac agcaaacgcc
     8821 tcattcaatt cccacaggcc gatgtcgccg attttcttgc cagtctgctt gagcaatttc
     8881 gggatggcat agactggccc gatgcccatt tcatcgggct cgcatccggc cacggcgaag
     8941 ccgcgaaaga tccccatagg ctttagtccc ctgcctgccg ccacgcgatc gctcatgacc
     9001 acttgcgctg atgcaccgtc ggaaaattga ctggcattgc cggccgcaat aacgcctccc
     9061 tcaatagccg gcttgatctg ggacacgccc tcgtaagtgg tgtcgggccg gataccctca
     9121 tcttcgctgg cggtgacctg ccgcgtggat tcctggccag tgttcttgtc aatgactttc
     9181 atcgttgtgg aaatcggaac gatctcgtct tttgtcttgc ctgccgctcg ggcagcggcg
     9241 gcacgctgct ggctttgtac gccatagcga tcctgcgctt cacgtccgat cttgtatcgt
     9301 ttcgagacca tctcagcagt cgccagcatg ggccagtaga tttccggctt gtgctgctcg
     9361 agccagtttt ctctcagcat gtgtttgttg gcctcgttct ggacacaaga tatggattcg
     9421 acgccgccgg cgacaaagat gttcgcttcg cctgcaagaa tgcgctgagc cgccaaagcc
     9481 accgtctgca gtccggacga gcaaaagcga ttgaccgtca tgcccgatac ggttaccggc
     9541 atgccagcgc gcagggcgat ctggcgcgcg atattttgac cggttgcgcc ttccggattg
     9601 gcgcagccca tgatgacgtc ttcaacctcg gccggatcga gtttcgcgcg ctcaagtgcg
     9661 gccttgacga catgtccgcc catggttgca ccatgggtca tattgagcgc gcctttccag
     9721 cttttgcaaa gcggtgtacg tgcggtggaa acaatcactg cgtccatgta attactcctt
     9781 cgcaaatgga tgaatcaaag ccttgctccg gcaagcatga cggcttcaaa acatgtttcg
     9841 ctattgattg aacttcttgc cttcggcggc gagtctttcg agcagcaccg cgggtttcca
     9901 gtattcgcca tgtgcggaat gataggcctg cacggatgcc agcactttgt ccaacccaat
     9961 cgtatcggcg tagaacatcg gccccccgcg atagcgcgga aaaccataac cgtagatata
    10021 aacaatatcg atatcactgg ctcgcaatgc aatgccttcc tccaagattt ccgcgccttc
    10081 attgacgagg gcgaaaatgc agcgttcaag aatttcctgg tcggtaattt gccgtcgctc
    10141 gataccggcc tccctggaag cttcgacgat cagttcttcg acaattggat cgggaatcgg
    10201 attgcgattg ccggcctcgt agcgatacca tcccgcgccc gtcttctgcc cgaagcggcc
    10261 gagttcgcag atctgatcgg ctactttcga atagcgcaga tgcgcgggcc gcgtggctgc
    10321 ctgacgcttg cgtatatacc agcccacgtc gagcccagcc aggtcaccga cgcgaaaagg
    10381 gcccatggca aaaccaaagt cctctattac cttgtccacc tgctgaggca gtgccccttc
    10441 ttcgagcaaa aaacccgcct cgcggaaata tccgtgcagc atgcggtttc cgatgaaacc
    10501 gtcgcagacg ccagcaacca cgccgacctt cttgatagct ttggatagct tcatggcgct
    10561 ggcgagtact tcttttgatg tttttctggc acgaaccact tccagcaact tcatgacgtt
    10621 ggcagggctg aaaaaatgca ggccaagcac atcttgtggg cgcgaggtca ttgccgcgat
    10681 ctcgttgaca tccagcgtcg aggtattggt ggcgaggatc gcacccggct tgcacacctg
    10741 gtcaagtttt tggaatacct gctttttgac ggccatgtcc tcgaacaccg cctcgatgac
    10801 gatgtccgcg ttcttgatat cttcgtatcg cgtcacgccg gtgaacaggc tcatgcgctt
    10861 gtccatggct tcctgcgtca accggccctt ggacaccgtg gctgcgtaat tcttgcgtac
    10921 cgtgccgagc cctttatcga gtgcttcctg gttcaactca agcattttga ccgcgatgcc
    10981 ggcattggca aaattcatcg cgatgcctcc gcccatggtg ccggctccaa tcagcgctgc
    11041 cgattttata tcgtgtgttg gcgtgtcctc tggcacgtcg ggaattttgc tgacctggcg
    11101 ctccgcgaag aacagatgcc gtaatgcgcg tgactcatcg ctctttacta gcttttcaaa
    11161 ttgctgacgc tcgaatgaaa ttcccttggc aaatggctgt gtcacggcag cctggacaca
    11221 tgcggcaata gccagtggcg cgggatagcc gcgccagttc ttcgcaatgc gctcgcgtgc
    11281 agcctggaaa aaagactccg caggctgatc cagtgcagga ctgagctggc tggttcgtcg
    11341 caccccgcga ttgtccttca gcagtcgatt tgcatacgcg atggcagcct ggcgaagatc
    11401 accggtgaca acctcgtcca caagtccagt gtccttagcc atctcgctgg agattggatc
    11461 gccggaaacc atgaactcca aagccttcgc cacgcccatc agtcgcggag cgcgctgggt
    11521 gccgccagcc ccgggcagga tgcccagctt gacctctggt aaagcgattt gcgcgccggt
    11581 caaggccaca cggtagtggc acgccagcgc caattccagg ccgcccccca tggttgggcc
    11641 ggaaattgcg gcaacgattg gcttgcttac ggtgtccata tagccaagga cgtcgggcaa
    11701 cattgctttg ccgggatcgt agcccttgcc aaattcgctg atgtcggcac cgccagaaaa
    11761 attccgtccg ccaccaataa ttacgaatgc gcgcacgtga tggtcatgat cgccttcctt
    11821 gaaagcatcc aaaatgcctt gtaccacgcc cttgttcacc gacagcgcgt tgaccggcgg
    11881 gttgttcagc gtgatgacgc caatgctgcc ctgcttctga taatcgacta gagacatgat
    11941 gagaatcctg ttagtaagcg acgctacgga tgaaattgtg cagtgataga aacatgtctc
    12001 gtttcttttc ttgctgctcc tgtgctccgt gagcgctgtg tcagattgaa gtcagacttc
    12061 cagccattcc ttgcgaaccg cttcgttggc acgcaacccg tccggtgtgc cctcgaacac
    12121 gatcttgcca tggcccatga cgtagagccg ctgggatatt ttcatggcga tcgtaagttt
    12181 ctgctcgacc agcaggatcg atacgccgcg attcttgatc tcctcgaaca agcgggcgac
    12241 ctgctcgact atttttggcg ccagcccttc cgtgggctca tcgatcatga ccagatcggg
    12301 atcccccatc aaggtgcggc acaaagtcag catctgctgc tccccgccag acatcacccc
    12361 ggccggtgct tcggcgcgct ccttcagccg gggaaacaag gcgtacatat cgtcaacgct
    12421 ccaccggcct cgctgtcgcg cgcgcttcat cccaagcaga agattttgtt tgaccgtgag
    12481 cgagggaaaa atctcgcggt tttccggcac gtagccaagg cccctgcggg cgaccatata
    12541 ggttttttgg ccgttgattt cctcgccctt gaattttacc gagccggtcg agttgacctg
    12601 acccatgatg gatttgatcg tggtggaacg gccgacgccg ttgcggccaa gcaaactaac
    12661 aatttcgccg gcgccgactt tcaaatgcac accatgcaaa atgtggcttt tgccgtagta
    12721 ggcatgcaga ttattaactt ccagcattgg tcgagacctt ggggatttac cgcagctagc
    12781 ccgcgctaat gttgttcggt gccaaggtaa gcctcttgga ccgcgctgtt gccgcgaatt
    12841 tccggaggcg ttccactggc gatgacttct ccgtacacga gcaccgaaat ccggtctgcc
    12901 agattgaaga cgacgctcat gtcatgctcg accatgatca atgtgcgccc ttcagtcacc
    12961 ttgcgaatca gttcgactgc ttcatcagtt tcactgcggc tcatgcccgc cgtcggttca
    13021 tcgagtagga cgacgttagc acctcccgct atggttatgc ctatttcaag cgcgcgctgc
    13081 tccgcatagg tcagcacacc ggccaggcgg tcgcggcgtt ttgccatgcc aatgcgttcg
    13141 acgatgtcgc tcgtgcgctc gcgcaccgct tccagcgcgt ccacgtggtg ccagaacgaa
    13201 tatttatagc cttgcgacca tagcactgcg cagcgtacgt tctcgtacac ggacatgcgc
    13261 ggaaaaatat tcgtaatctg gaaactgcgc gcaaggcctt tgcggttgat gatctccggt
    13321 tccagtccgg taatgtcttc gccatgcaga tcaatccgac ctgaagtcac tccgaaacgc
    13381 ccgctgatga ggttgaacag cgtggacttg cctgcaccat tgggaccgat gatggcatgc
    13441 cgttctcctg gctcgatgct caggttgacg ccattgatga tggaggtccg tccaaagctt
    13501 ttgcgaacat cgtgcaatcg cagcgcggcg acgggcattg ctaaaccgct cccttcttct
    13561 ggagatccaa ggtcagatca tcccaggttc ggctgacgaa cgtccttgcg ccataaacca
    13621 gcgcaatgcc gatgaggaaa acggccgcgc aaccgatcca gtagcgagct tgcatgggat
    13681 caagggccag accgaaatag gagaacgcct tgctggacg
//
LOCUS       DATMZQ010000469         9440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_939928, whole genome shotgun sequence.
ACCESSION   DATMZQ010000469 DATMZQ010000000
VERSION     DATMZQ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939928"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VN496_14885"
     CDS             complement(<1..480)
                     /locus_tag="VN496_14885"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXF18290.1"
                     /translation="MNNLRAVARAALVLCLLLFAANAHAHSMVKGVGDLYAGLLHVLT
                     ALEHVLPFIALSLLAGQRGIKIPVEGVLIVFPVALMLGAATALWVPPLRGLAFFNIAS
                     AIVLGGLVAAAWPLPRMVFYGLCIIFGISHGFANGEAISEGTKVYLFILGVGLAGLAV
                     "
     gene            complement(488..1108)
                     /locus_tag="VN496_14890"
     CDS             complement(488..1108)
                     /locus_tag="VN496_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXF18291.1"
                     /translation="MNAEPCTLVRPAQWFLIAYFLAFPTLVFAHVGQGDVSGGFVAGL
                     EHPVMGLDHVVAMVAVGIWGAQLRAPAIWVLPVTFPLVMSFGGVLGGLGVPIPGIEIG
                     IAVSAIVLGAMVAFAARPPLWAAAIIVGIFAIFHGYAHGAELPESANAISYAMGFVVA
                     TGCLHALGILIGVINKWRAGTRVLRAGGVLIALCGVYFLVSHFRGG"
     gene            complement(1175..3211)
                     /locus_tag="VN496_14895"
     CDS             complement(1175..3211)
                     /locus_tag="VN496_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXF18292.1"
                     /translation="MAAAFGAMALPGLLVAAEGDITAPEVEVVGTYETGVGTSDAASE
                     GTVTARRIETRPALRPGEVLEYIPGLIVTQHSGDGKANQYFLRGFNLDHGTDFSITAA
                     GMPVNMPTHAHGQGYADLNFLIPELVSGIDYRKGPYYAEVGDFSSAGAADIHYATSLP
                     HSIGSVTLGEHDYKRALLAGSPNVGGGTLLYGIELMGQDGPWDNPENFRKLNTVLRYS
                     RGDKADGIAITGMAYKANWDSTDQIPQRAVASGLLDRFGAVDPSDGGETSRYSLSYEQ
                     HHDFAGGRAMVDVFAIRSSLTLWSNFTFFLDDPVNGDQFEQHENRIVLGVHPRAEFYG
                     KLAGMDSIFKIGLQARRDDIDPVALYHTVERQIIGTTRSDTVVESSVGLYADETLQVA
                     DKFRMILGVRGDRYDFDVNSDIPQNSGRVSDSIVSPKLSFIFGPWSKTEYFINYGTGF
                     HSNDARGTTITVDPSNPSVPAEKVTPLVRSFGSEVGLRSELVPHLQTSLSLWQLRLES
                     ELLFTGDAGTTEPSRPTKRTGIEWSNHYRANSWLLLDLDLSVSRARFADQDPAGDEVP
                     GSIEQVASFGVTVDSFGPWYGMLQYRYFGPRPLIEDNSVRSQSTQVTNLRVGYKIDPK
                     WRVHLDVFNLFDREDHDIDYFYASQLRGEPAPVDDIHFHPIEPRTLRFTLTGYF"
     gene            3429..3887
                     /gene="nikR"
                     /locus_tag="VN496_14900"
     CDS             3429..3887
                     /gene="nikR"
                     /locus_tag="VN496_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HXF18293.1"
                     /translation="MDRFTISLNEDLARQFDELIGQKGYQNRSEAVRDMLRDRLESAR
                     LDKNESPYCIASLSYVYNHHARDLTERLTDLQHQHHHLVLSSMHVHLDHDNCMETVIL
                     RGATGEVSRFANELMAVREVRHGKVNLVPVEMDDHSHRPHQYHVHTRPRT"
     gene            3988..5139
                     /locus_tag="VN496_14905"
     CDS             3988..5139
                     /locus_tag="VN496_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HXF18294.1"
                     /translation="MASQPLLFSPITLRGITLKNRVVISPMCQYSAHEGFADDWHLVH
                     LGKMATGGAGLIFTEAAAVVPEGRITYGDLGIWSDAHIAPLKRITQFIKANGAVPAIQ
                     LAHAGRKASMQRPWHGNGPINDTDRARGEEAWSIVAPSPIAMDTGWLVPHELSIDDIR
                     GVQARWREAAERSLSAGFEVLEMHSAHGYLSHEFLSPLSNKRTDRYGGDLHGRMRYTL
                     ELAEVLRDAWPLDKPLFVRISSVDGIEGGWTLEDTVTLTKELGVRGVDVIDCSSGGLL
                     GSATAARIKRNPGFQVPFADRIRKEAGIKTMAVGLILDAMQAEQILEDGQADLIAIGR
                     QSLYNPNWALHAEVALGAQGEYASWPVQYGWWLDKRQHILTDEAKRKIA"
     gene            5247..5630
                     /locus_tag="VN496_14910"
     CDS             5247..5630
                     /locus_tag="VN496_14910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF18295.1"
                     /translation="MAIDGMNHFTVLTKDLDATRDFYIGILGLQEGYRPPLGFPGMWL
                     YAGAQPILHVVAGRPLPDPPAGVLDHMAFSASDLGAVTSKLKEKGIKYDLRKQPDSGV
                     WQLFCFDPSGARVELDFSAKETALA"
     gene            complement(5670..6575)
                     /locus_tag="VN496_14915"
     CDS             complement(5670..6575)
                     /locus_tag="VN496_14915"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXF18296.1"
                     /translation="MDRLTAMGLFVRIVETGSFSAVAREMNMTQPTVSKQLTALERQL
                     KTRLLNRSTRQLSLTEAGSAYFESSKRILDTVTEAEANLGVLQTQLSGVLRINSSIGL
                     GQMYLGPLVLKFQSMHPGLMIDLAYADRFVDLVEEGVDVAIRIGRLMDSGLSARRIGT
                     SERCTVATAGYLKSKGTPKTPQDLVNHNCILYAYLSTGSEWIFNGKDGEIRVKVSGTL
                     RANNGEAIKQAVMADLGIAISPTWLIQEDLKAGRVQEILRDFSPAPAEINAVYPSARH
                     VSAKVRAFTEFLKAEFEKIPALRVR"
     gene            6705..7103
                     /locus_tag="VN496_14920"
     CDS             6705..7103
                     /locus_tag="VN496_14920"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HXF18297.1"
                     /translation="MNIIKTLVAGLLLLASTAFAEEWSFVAQQGNEIVFVDRTSIFEV
                     GDLKKARVLRSFSTVQTIGDTAFPHKSEILLYAVRCEDRSLGFEQWTMTAGEIGTGAT
                     VWAGQIEQPALYKDPSDPTVLGMIASVCNS"
     gene            complement(7156..7422)
                     /locus_tag="VN496_14925"
     CDS             complement(7156..7422)
                     /locus_tag="VN496_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18298.1"
                     /translation="MHDSKGFVIVATIAGVAFGSPASAQQDAAQKATEGSIDHWIEYY
                     RGQQSKSIAPPRQETVPNPTPPESANSVRAPREPGEPAASTTPK"
     gene            complement(7398..7733)
                     /locus_tag="VN496_14930"
     CDS             complement(7398..7733)
                     /locus_tag="VN496_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18299.1"
                     /translation="MSKSFLWLCALLVSVPVHADRISRMNQTELCTYTAQLKVAAYYF
                     FEQGKPREEVNIKWHGDETQNEMEFVNRTVEEAYAWLAKWKENNTELLSAQSFGDRVY
                     LACMTQKDL"
     gene            7896..8288
                     /locus_tag="VN496_14935"
     CDS             7896..8288
                     /locus_tag="VN496_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18300.1"
                     /translation="MNRRIIRAIGLFALGSACAVPALAHDLTLKECNEGGEFIRNAAL
                     ARDNGMTRAFFVDKLEEDLMLIRAFPPQLRWFAQDASDEKLLTQAVLQVFDAPLKPEQ
                     HEAAFIMNCTEAAASIEDDQVDKSGDSI"
     gene            complement(8371..>9440)
                     /locus_tag="VN496_14940"
     CDS             complement(8371..>9440)
                     /locus_tag="VN496_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXF18301.1"
                     /translation="PGAHFEGTDKTIHEVEIFLGVFIGAVTFTGSIVAFGKLQGTISG
                     KPLTLPGRHWLNLVAIIASVWAGYLFLTAPGTEAGLIPLLIMTAIALFLGVHLVAAIG
                     GADMPVVVSMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRNFV
                     AVIMGGFGTASGSASAVPQGEVIATTAEEVAGLLSEAKEVVIVPGYGMAVSQAQGVVS
                     EITRRLRAKKINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDLPHADVV
                     LVIGANDIVNPGALEDPNSPIAGMPVLEVWKAKTVVVFKRSMATGYAGVDNPLFYKEN
                     TRMLFGDAKKMLDGILQHLQV"
BASE COUNT         2084 a         2742 c         2685 g         1929 t
ORIGIN      
        1 taccgcaagt cctgcgagcc ctaccccaag aatgaacaag tacaccttgg taccctccga
       61 tatggcttcg ccgttggcga atccgtggct gatgccgaag ataatgcaga gtccatagaa
      121 caccattcgc ggcagcggcc acgcagcggc gaccaaaccg cccagtacga tagcagaagc
      181 gatattgaaa aaagccagtc cccgcaaagg gggcacccat agtgcggtcg ctgcaccgag
      241 cattagcgca accggaaaga cgatcaacac gccctctacg ggaattttta ttccgcgctg
      301 tcctgccagc aggctcaggg caatgaaagg caggacatgt tcgagggccg tcaagacatg
      361 caacaagccg gcgtaaagat ctccaacgcc cttgaccatg gaatgcgcat gcgcattggc
      421 tgcaaacaac agcaggcaca gcacgagagc cgctcgcgcg accgcgcgca aattattcat
      481 tcggtggtca cccgcccctg aagtgcgaaa cgaggaagta aacgccgcac agcgcaatca
      541 gcacaccacc cgcgcgtagc accctggtcc ccgctcgcca cttgttgatc acgccgatca
      601 ggatgcctag agcatgcaga catccggtcg cgaccacaaa gcccattgca taagaaatcg
      661 cattggcaga ttccggaagt tctgcgccat gcgcgtatcc atggaagatc gcgaaaatac
      721 ccacaatgat tgccgctgcc cacaaaggcg gacgggccgc gaatgcgacc attgcaccca
      781 gcacgatcgc ggaaacggca atgccgatct caatgccggg tatcggtacg ccgagaccgc
      841 ccagcacgcc cccgaatgac atgaccagcg gaaaagtaac aggcaatacc catattgccg
      901 gggcacgcaa ctgcgctccc caaataccga cggccaccat tgccaccaca tgatccaggc
      961 ccatgaccgg gtgctcgaga ccggcaacaa aaccaccgga cacgtccccc tgcccaacgt
     1021 gtgcaaaaac gagcgtcggg aaagccagga aataagcgat caaaaaccac tgcgccggac
     1081 gaacaagcgt gcatggttct gcgttcacgt aatggacctt acaaggatat aaaaggaggc
     1141 ttgggtgcgg cgaaacggcg agcgcgccat gttgtcagaa atagccggtc aaggtaaatc
     1201 gcagggtgcg tggctcgatg ggatgaaaat ggatgtcatc cactggcgca ggctcgccgc
     1261 gcagctggga cgcatagaaa taatcgatgt catggtcctc gcgatcgaac aagttgaata
     1321 catcaaggtg cacacgccac ttcggatcga ttttgtagcc cacacgcagg ttggtgacct
     1381 gcgtcgattg cgaacgcacc gagttatcct cgatcagcgg acgtggtccg aaataacggt
     1441 attgcagcat gccataccaa ggcccgaatg aatccaccgt gactccaaag gaagccacct
     1501 gttcaatgga gccgggcacc tcgtcgccgg ccggatcctg gtccgcaaac cttgcccgcg
     1561 agacggagag atcaaggtcc agcaacagcc atgaatttgc ccggtagtgg ttgctccatt
     1621 cgattccggt ccgcttggtg ggccggcttg gttcggtcgt gccggcgtca cccgtaaaaa
     1681 gcagttccga ttccaatcgc aattgccaga gtgagagcga cgtttgcaga tgcggtacaa
     1741 gttcggagcg caagccgact tccgaaccaa acgatcgcac gagcggtgtg accttttcgg
     1801 ccggaaccga aggattgctc gggtcaaccg taatggtcgt gccgcgtgcg tcgttgctgt
     1861 ggaagccagt cccatagttg atgaagtact cggtctttga ccacggtccg aaaataaagc
     1921 tgagtttcgg cgaaacaatc gaatcgctca cccggccgga gttctgcggt atgtcgctgt
     1981 tcacatcgaa gtcgtagcgg tcgccgcgca cgccgaggat catgcggaat ttatcagcga
     2041 cttgcagcgt ttcgtcggcg tagaggccga cgctgctttc cacgactgta tcggagcgtg
     2101 ttgtacctat tatctggcgc tcaacggtgt gatacagcgc gacgggatca atgtcgtcgc
     2161 gccgcgcctg caggcctatt ttgaatatgc tgtccatgcc ggcgagtttg ccgtagaact
     2221 ctgcgcgcgg atgcacgccg agcacaatgc gattttcgtg ctgctcgaac tggtcgccat
     2281 tcacggggtc atccaggaaa aatgtgaagt tggaccacaa cgtcaacgat gaacgaatcg
     2341 cgaacacatc taccatggcg cgcccgcctg cgaaatcgtg atgctgctcg taggaaaggg
     2401 agtaacgcga agtttcgccc ccatcactcg gatcgacggc gccgaagcga tcaagcaatc
     2461 cactcgcgac tgcccgttgc ggaatctggt ccgtggaatc ccagtttgct ttgtatgcca
     2521 tgcctgtgat ggcaattccg tctgccttgt cgccacggct gtaccgcaac acggtattga
     2581 gtttcctgaa attctcggga ttgtcccatg gaccatcctg acccatgagt tcgatgccat
     2641 aaagcagcgt gcccccgccg acgttcgggg aacctgccag cagcgcgcgc ttgtagtcat
     2701 gctcgccaag cgttacggaa ccaatggaat gcggaagcga agtcgcgtaa tgaatgtcag
     2761 ccgcgcctgc ggaactgaag tcaccgacct cggcgtagta cggtcccttg cggtaatcga
     2821 tgccagaaac gagctccgga atcagaaagt tcagatcggc ataaccctgg ccgtgcgcat
     2881 gtgttggcat attgaccggc atgccggcgg cagtgatgga aaaatcggtg ccgtgatcaa
     2941 ggttgaagcc gcgcaggaaa tattgattgg ccttaccgtc tccgctgtgc tgcgtaacga
     3001 tcaggcccgg aatatattcc agcacttcgc cgggacgcag cgcgggacgg gtctcgattc
     3061 gccgcgccgt cactgtgcct tcactcgcgg catcggaggt cccgaccccg gtctcgtagg
     3121 tgccgaccac ttccacttcc ggggcggtga tgtcgccttc ggcggctaca agcaggcctg
     3181 gcaacgccat ggcaccgaac gcagccgcca tggcacacgc cgccaattgc ctggttggat
     3241 gacgcgtatc ccccgaagaa ctccaacaag ccattgttat tatttgtttt aggaagtaag
     3301 ctgtatttcg cttttttcat aatatagggt cgcaagcgga ttatgtaaag cgtggcaatt
     3361 ggggattctc cggtcatcct atttttttcg gtaacatctc acgcggggac gacaggagga
     3421 gtttttggat ggatcgcttc acgatttcgc ttaacgaaga tcttgccagg cagtttgatg
     3481 aattgatcgg acagaagggt taccagaacc ggtccgaagc cgtccgcgac atgctgagag
     3541 accgactcga atcggcgcgg ctcgacaaaa acgaatcgcc gtattgcatc gcttcgctgt
     3601 cgtacgtcta taaccaccac gcgcgcgatc tgaccgaacg cctcacggac cttcagcatc
     3661 agcatcatca tctcgtacta tcgtcgatgc atgtccacct cgaccacgac aactgcatgg
     3721 aaacggtaat cctgcgcggg gcgaccggcg aagtcagcag attcgcgaac gagttgatgg
     3781 cggtgcgcga ggtgcgtcat gggaaagtta atttggtccc ggttgaaatg gatgaccatt
     3841 ctcaccggcc gcatcaatat cacgtgcata cccggcccag aacctgattc ggcgtgctgt
     3901 agcccgcata ccattacgga aacaaagcag ctattccgta catcccgttg tcccaggtac
     3961 gccacccatc gcatcaaaag gaatccaatg gcatcgcaac cgcttctgtt ctctccgatc
     4021 acgctgcgcg gcattacgct taagaatcgc gtcgtcatat ccccgatgtg ccaatacagc
     4081 gcgcatgaag gttttgccga tgactggcat ctcgtgcatc tgggcaagat ggccaccggc
     4141 ggggccgggt tgatctttac ggaagccgcc gcagtggtac cggaaggccg cattacctac
     4201 ggcgatctgg gcatctggtc ggacgcgcac attgcgccgc tgaaacgcat aacgcaattc
     4261 atcaaggcca atggcgcagt accggccatc cagctcgctc atgccggccg caaggcttcc
     4321 atgcaaaggc cctggcatgg caatggtccg atcaacgaca ccgaccgcgc gcgcggagag
     4381 gaggcctgga gcattgtcgc gccgagcccg attgcaatgg ataccggctg gctggtgccg
     4441 cacgaactga gcatagacga catccgcggc gtgcaagcgc gctggcgtga agccgccgag
     4501 cgatcgctct cggccggttt tgaagtcctg gaaatgcatt cggcgcacgg ttatctcagc
     4561 catgaatttc tgtccccgct ctcgaacaag cggaccgacc gctatggcgg cgatctgcat
     4621 ggccgcatgc gctatacctt ggaactggca gaggtattgc gcgacgcctg gccgctcgac
     4681 aaaccactgt tcgtacgcat ctcatctgtc gatgggatcg agggcggatg gacgcttgag
     4741 gacacagtga cgctgacaaa agaattgggc gtgcgcggcg tcgacgtgat cgattgttcg
     4801 tctggcggcc tgcttggatc ggccaccgcc gcacgcatca agcgtaatcc tggcttccag
     4861 gtgcccttcg cggatcgaat tagaaaagaa gccggcatca agaccatggc agtgggtctg
     4921 atcttggacg caatgcaggc ggagcaaata ctggaggatg gccaggccga tttgattgcc
     4981 atcggccgcc agtcgcttta caacccgaat tgggcgctgc acgcggaagt agcgctgggc
     5041 gctcagggag aatatgcgtc ctggccggtg cagtacggct ggtggctgga caaacggcag
     5101 cacatcctca cggatgaggc gaaacgaaag atcgcgtaat gatgggaata gcatggcggt
     5161 atgagtgtcg taaaatgata taacccacga cagtcacgag tagttgcgca attacggcag
     5221 tcacggcttt ctcacggagt ttcgccatgg ccattgatgg aatgaatcac tttacggtgc
     5281 tcactaaaga tctcgatgcg acgcgcgact tttatatcgg catcctgggc cttcaggaag
     5341 gatatcgccc gccgctcggt tttcccggca tgtggctcta tgccggcgcg cagcccattc
     5401 tgcatgtggt ggccggacgt ccgctgcccg atcctccggc gggggtgctc gatcacatgg
     5461 ccttttccgc ctcggatctt ggcgctgtca cttcgaaact gaaggagaaa ggcatcaagt
     5521 acgatttgcg caaacagccg gattccgggg tctggcaatt gttttgtttc gatccaagcg
     5581 gcgccagggt ggagctggat ttttctgcga aggaaaccgc gctcgcctga ccgcaaagat
     5641 tggcgcgcta ttgcaggggc ctgccggcgc tagcgcacgc gaagcgccgg aattttttcg
     5701 aattcggctt tcagaaattc cgtaaaagcg cgcaccttgg ccgatacgtg ccgcgcggaa
     5761 ggatagaccg cgttgatctc ggcaggagcc ggggaaaagt cgcgcaggat ctcctgcacc
     5821 ctgcccgctt taagatcctc ctggatcagc caggtcgggc tgatggcaat gccaaggtcg
     5881 gccatcactg cctgcttgat tgcttcaccg ttattggcgc gcaaagtgcc cgaaaccttg
     5941 acgcgaattt caccatcctt cccgttgaag atccattcgc tgccggtcga aagatacgca
     6001 tacaggatgc aattatggtt gaccaggtcc tggggcgtct ttggcgtgcc cttgctcttc
     6061 agatagcccg cggtcgctac cgtgcaccgc tccgacgttc cgatgcgccg cgccgaaagg
     6121 ccggagtcca tgaggcgccc gatgcggatc gcgacatcga cgccttcctc aacaaggtcc
     6181 acaaagcgat ccgcgtaggc aaggtcgatc atcaggccgg gatgcatcga ctggaacttc
     6241 aaaaccagcg gacccaggta catctggccc aggccgatgg acgaattgat ccgtagtacc
     6301 ccgctgagct gggtttgcag tacgccaaga ttcgcctccg cctcggtcac cgtgtcgaga
     6361 atgcgcttcg aactttcgaa gtaagcggag ccggcttcgg tgagcgatag ctggcgtgtg
     6421 cttcgattca gcagcctcgt tttgagttgg cgttccagcg ccgtgagttg cttgctgacg
     6481 gtaggctggg tcatgttcat ctcgcgcgcc accgccgaga agctccccgt ctcaacgata
     6541 cgcacgaaca atcccattgc agtgagtcga tccatcgttc cccgatcatg ccaaagcggc
     6601 atgaatcata tgcgacttgc cggtctatcc attgcgcatg ttaaagccta catttgctgc
     6661 catgcaacaa tccagttgcg ggccggaatc tgaaagggaa cgtcatgaac ataataaaga
     6721 cactagttgc ggggctattg ctcctggcat cgacggcgtt cgccgaagaa tggagttttg
     6781 tggcacaaca aggcaacgag atcgtattcg tcgacaggac cagcattttc gaggtgggcg
     6841 atctcaaaaa ggcacgcgtg ctgcgcagct tcagtaccgt gcaaaccatc ggcgacacgg
     6901 cttttccgca caaatccgaa attttgttat acgcagtgcg ctgcgaagac cgcagtctgg
     6961 ggtttgagca atggaccatg acagccggag aaatcgggac cggggccacg gtatgggccg
     7021 gacagatcga gcagccggca ctgtacaagg atccgagtga tccgacagtc ctcggtatga
     7081 ttgcgtccgt atgcaacagt tgatgtcgta gtcgggtctg attcaactgg aggtgcgcgt
     7141 cgcgcacctc ctttttcatt tgggtgtggt gctcgcggcc ggttcacccg gctcccttgg
     7201 cgcacgaact gaattggctg actcgggtgg cgtagggttc ggtaccgttt cttgccgcgg
     7261 cggcgctatc gacttggatt gctggcccct gtagtactcg atccagtgat cgatgcttcc
     7321 ttccgtggct ttctgtgcgg catcctgctg cgccgacgcg ggactcccga atgcaacgcc
     7381 ggcaatcgtc gcgacgatca caaatccttt tgagtcatgc atgccagata caccctgtct
     7441 ccgaatgact gggccgacaa caattcggtg ttattttctt tccactttgc cagccaggca
     7501 taggcttcct cgaccgtccg gttaacgaac tccatttcat tctgcgtttc atccccgtgc
     7561 catttgatgt tgacctcttc acgcggcttg ccttgctcga agaagtaata ggctgccact
     7621 ttgagttgcg cggtataggt acacaattcc gtctgattca tgcggctgat ccgatcggcg
     7681 tgaaccggta cggataccag caatgcgcat agccacaaga aacttttgct cattgcaaac
     7741 ctcttaaaag tgagggtgcc cgacgctgcg gcgcaacgcg aaaatacgat agcggccgca
     7801 gggcaagggc tacaattgga ttgcccggcg gtaggaaaac tggctgcagc atgttttatg
     7861 accggccgtc ggctgagtta gcgatggtga ccacgatgaa tagacggatc attcgcgcaa
     7921 ttggtctttt cgccctgggc agtgcttgcg ccgttccggc gttggcccac gatcttacgc
     7981 tgaaggagtg taacgaaggc ggtgagttca tccgcaacgc tgcgctcgca cgcgataacg
     8041 gcatgacgcg tgcctttttc gttgacaagc tggaagaaga cctgatgctg atccgcgctt
     8101 ttccgccgca gctgcgctgg ttcgcgcagg atgccagcga tgaaaaactg ctcacccagg
     8161 cggtattgca agtgttcgat gcgccgttga agccagagca gcacgaagcg gcattcatca
     8221 tgaactgcac ggaagccgca gcgtccatcg aagacgatca ggtcgacaaa tcgggcgaca
     8281 gcatttgagg tgtgactccg cgcgctgacg tttgctgaaa tagccaggcc ttcccttgca
     8341 ccaggcccaa tgaatccgca gcgcggggcc ctacacctgt agatgctgca ggatgccgtc
     8401 cagcattttc ttggcatcac cgaacaacat gcgggtgttc tccttgtaga aaagcggatt
     8461 atctacgccg gcatagcctg ttgccatgct gcgcttgaac accacgacag ttttggcttt
     8521 ccatacctcc agcaccggca tgcccgcgat gggactattc ggatcttcca gtgcgccggg
     8581 attcacgatg tcattggcgc cgatcaccag gaccacatct gcgtgcggca agtcgtcatt
     8641 gatctcgtcc atctcaagca cgatgtcgta gggcactttg gcttctgcga gcagcacatt
     8701 catgtgcccc ggcaagcggc ctgcgacagg atgaatgccg aagcgcacat taattttctt
     8761 tgcgcgcagg cgcctggtga tttcgctaac cacgccctgc gcctgggaga cggccatgcc
     8821 gtatccgggc acgatgacca cctctttcgc ctcgctcagc agacccgcta cttcttccgc
     8881 agtcgtcgcg atcacttcgc cttgaggtac tgcggacgcg gatccacttg cggtgccgaa
     8941 gccgcccatg atgaccgcaa cgaagttacg gttcattgca cggcacatga tgtaggacag
     9001 aatcgctccg ctggatccga ccagcgcgcc ggtaacgatc agcaggtcat tggacaacat
     9061 gaaaccggta gccgcggcag cccacccgga atagctgttc agcatcgaca cgactaccgg
     9121 catatcggcg ccgccgatcg cggccaccag atgcacgccc aggaacagcg cgatcgcggt
     9181 catgatcagc aaaggaatga ggcctgcttc cgttcccggt gcagtcaaga ataaatatcc
     9241 ggcccacacg gacgcgatga tggccaccag attcagccag tgacgtccgg gcaacgtcag
     9301 cggtttgccg gaaatggtgc cctgtaattt tccgaatgcg acgatggacc cggtaaacgt
     9361 caccgcaccg atgaacacgc ccagaaaaat ttcaacctcg tgaatggtct tgtccgtacc
     9421 ttcgaaatgc gcgcccggat
//
LOCUS       DATMZQ010000470        13004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1591115, whole genome shotgun sequence.
ACCESSION   DATMZQ010000470 DATMZQ010000000
VERSION     DATMZQ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591115"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1074
                     /locus_tag="VN496_14945"
     CDS             187..1074
                     /locus_tag="VN496_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXF18302.1"
                     /translation="MIHGWPESWYSWRHQIGPVSEAGYRVVVPDVRGYGGSDAPAAIE
                     AYDMENMIADVLGLIDHFSEQQAILFGHDWGAPIVWNTTALHADRVRAVAALSVPYAP
                     RGKISSIELWKQIYAGRFFYQLYFQQPGIAEAEFEADVRTSLRKIYYAGSGDSPRGMF
                     RADKRPDANMLGDLPHPATFPDWLTEQDLDYYAAEFKKSGFRGPLNRYRNQQRDWQRL
                     TALSGARISRPACFIAGRRDGVLKFVPDFDMVENMKRWTDDLRICEIMEGAGHWIQQE
                     RPAQVTQLLLSFLAGLKKD"
     gene            1206..1550
                     /locus_tag="VN496_14950"
     CDS             1206..1550
                     /locus_tag="VN496_14950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXF18303.1"
                     /translation="MASSKTASGLEIEELVIGEGNVAASGQNVTVHYTGWLTNGSKFD
                     SSKDRNDPFRFGLGQGQVIRGWDEGVQGMKVGGKRKLTIPPELGYGARGAGGVIPPNS
                     TLVFEVELLGVK"
     gene            complement(1582..2892)
                     /locus_tag="VN496_14955"
     CDS             complement(1582..2892)
                     /locus_tag="VN496_14955"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXF18304.1"
                     /translation="MFEAKIRDLSRRDLWLTVIPVALLVLGVFVVALRFLEPAPPDSI
                     VLSTGPVDSGYHMFALRYRDILARDDVHLELRSSAGSQENIGRLLDDQSAVEVGFLQG
                     GSAYAANAPELVSLGSVYYEPLWVFYRGPEVHDFGGLRGKKLAIGPEASGSRALALQL
                     LAVNAAVMPPTMLLPDDGKQANEMLLRGKLDAVFIVGPPESPLVEQLALAPGIRLLDL
                     KRAEAYTRRFPSLTKLTLPQGVFDFVNNVPAHDVTLLSSTANLLAVDSLHPALAYLLM
                     RAASEVHGSAGLLRKAGEFPAPMNSEFPLSAEAQRYYKSGPPFLQRYLPYWAAVMVDR
                     LWLTLLPLLALLIPLGRIVPAIYRWRVRSRIYRWYAKLKELELELDDQSTPEQLRALL
                     ARLNEIERAVNRINTPLAYTDNLYAFRQNVNLVRQSVRDRLAAA"
     gene            complement(3179..3739)
                     /locus_tag="VN496_14960"
     CDS             complement(3179..3739)
                     /locus_tag="VN496_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18305.1"
                     /translation="MPTPRNPEDDFLDDPAKDDEADEEEEVVAGGRCPICEMLAGECD
                     HLVASIDLTYAEFVAGAIFAQERVILDLLERLVASEPDALKAAGAGPVLEQAATLIRG
                     EMDEGATAGDAVSIHYPQVMAALSYILQEEEDVTVTGTDAASDDDSSVVNFWAQEPEW
                     IVERVIERFQGLVEEIDEENDRRGVG"
     gene            complement(3975..4253)
                     /locus_tag="VN496_14965"
     CDS             complement(3975..4253)
                     /locus_tag="VN496_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXF18306.1"
                     /translation="MWFVYMVRCADQSLYTGIAKDVARRLAEHNSSGMLGAHYTRSRR
                     PVVLVYTEPARSRSVAAKREYEIKQLTRQAKDQLVRQSRLRIPAAQSG"
     gene            complement(4334..5017)
                     /locus_tag="VN496_14970"
     CDS             complement(4334..5017)
                     /locus_tag="VN496_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HXF18307.1"
                     /translation="MTDSNVPLLTPLEIRVLGVLAEKQLTTPDYYPLSLNGLVAGCNQ
                     KTSRDPVMEASEAEVQEALDSLKERKLVTNSWGASRRVVRYSHNLPQVLGTDSGTTAL
                     LVTLMLRGAQTTGEMRTACERLYRFADLSSVDAYLSEMVSRPNGPLVVKLAKQPGSRE
                     HRWAHLLGGPVDAQNASSAVALEAAGDESRTALQGEVAVLRDEVAALRRAVEELKSEV
                     ARTKGRPEP"
     gene            5125..6102
                     /locus_tag="VN496_14975"
     CDS             5125..6102
                     /locus_tag="VN496_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXF18308.1"
                     /translation="MNTENCAPPGSPGVCIDRRLFIKMAAALACTPLLDRVHASGLVR
                     PAAVPGGFAVVQLGPARMAPVVHFNGDRVFVSGTQTGWFAIVGIPLDAQPGRAAPLFI
                     EDPAGKSRVTYFTIGVKHYETEYLSMRSDYVDLEPENLARCETERSHLQRVLRTYSDA
                     SPSSLLLVPPCQGTRTSTFGLVRFFNGQSRGAHNGMDIAAPAGKPVFAAASGEVVDAG
                     DYFFSGRTVIVSHGRGFITLYAHLSEVKVRARKRVTAGQKIGKIGITGRATGPHLHFG
                     VYLNAAAVDPALFLLTDPRTQHLYSPSRRAPNSGEPFGAPLRETVIKQL"
     gene            6154..7023
                     /locus_tag="VN496_14980"
     CDS             6154..7023
                     /locus_tag="VN496_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXF18309.1"
                     /translation="MIAQYNPDKFESVLRRHFLQLVTAVSSLWIFHAQAGSLQKAAAV
                     PGGVAVVKIGSAPKPPTARVNGNRVLVTGSETEWLAVVGIALEARAGDKLALAVEFAD
                     GKTATLQVAIGTKQYATQRLTVKPGQVELSPEDLARYETERMHLGEIRKTFTEAAPDS
                     LELMQPCEGPRANTFGQRRIFNGQSRNPHNGMDIAAADGSPVLAAGEAKVIDVGNYFF
                     SGNTVILDHGSGLLTLYAHLSSVEVAPGDRVGTGARIGRVGATGRVTGPHLHFSVFLN
                     SVAVDPGIFLADM"
     gene            complement(7053..11585)
                     /locus_tag="VN496_14985"
     CDS             complement(7053..11585)
                     /locus_tag="VN496_14985"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HXF18310.1"
                     /translation="MTIQQRRLVALVDFAQHSLRTRGRVVSDVTSHDSFLLYEPQARE
                     IPGLLEGRRMDADDETWLRVPHPPGPQVPPQAESPWLSPWLNVGATVSAAPQLATEID
                     GAALITAGTHRDITGPDAASPAVDSHVCVSFQDYEFRNEVEAQYARYLEASWRPWADA
                     ESQRRRLANLYVGLFTLHQELAGALVEGQLELVWGMALCTARKAGSAISYPLITQTME
                     LSFNPETQAAELRPRDIAPRVEIDVFFHADTPAWSNLEKEAEQFLITTRDTLSPFAPA
                     SIEPLLDLARRTFESAAQQTGEPNQCEISSSWVLFARPRSSVAAVQDLERFRDQLSLL
                     DNESSLPQAVAALVTEPSSLVSPAALPAYRGVTAAYHAGAAQSTDAQDLFFPKPFNDE
                     QARIVQMLEISDGVVVQGPPGTGKTHTIANIICHWLANGRRVLVTSMRDPALAVLREL
                     LPHEIRPLAIPLLSADQDGVDQFERSIRKIAAEVQSLDTVTAARQVEGLVEMIDGFHG
                     KLKRIDTDLGRWAKLNVSRIDLGGETIDPQDAAREVMQGAGQYEWIPDGLGVGPQYIA
                     RFSAEDIARLREARRQLGEDIQYAGSALPAAEDFPDALALVRAHDDLQRYAKVTREAR
                     SSELAVPAGSGTESLLGARALAEQIARVKSLRDELTGLRLPWSEEAIARMRSGEPKRL
                     FDLLDALAADMDRAAAQRAQLLARPVIVPEAADTDAQFHQAVINLAEGRRPFGLIGLG
                     RPEAKKLLDMVRVNGSRPADMADWKAVLGHVELQKTRRALTARWNALATDIGFQTVLS
                     ADARGRLSAESQLVHYEKLKLLAREEAQLSRMAMELFPAWARAATAHQNSAVLSEVEL
                     ALAYHLTKSQLSQVSTVVNGLHRALEGKQGKIISRMREFLAGTLGNPAVDESALLEAW
                     NMLGAELSRLRSLAAPLAVVTVVTANIAASGAPKLAQKLRLMGTGFDDKLSTATMLRD
                     WRLRRLATHLQAIDAHDELKKLSAARVEVELDLARAYEDLVVKRSWLKLAENATPDVR
                     AALQAYLNAIQHLGKGTGKRAFRYRRDARAASAQAQHAVPCWIMPHHRVSESLPAQLG
                     CFDLVIIDEASQSDLSALPVLLRARKILVVGDDKQVAPQTVGIEEERINTLMTRYLAE
                     QVPLYAAQMSPERSIYDLAKVVFAHSGVMLKEHFRCVAPIIEYSKREFYEHELYPLRL
                     ANSSERLDPPLLDYLVGDARREGAINRAEIEFIVKDVRAFVADPKMQSRSIGIVSLLG
                     EEQALQIWQRLTEVLSPDTLRRHAVTCGDARQFQGRERDIMYLSMVCAPNDIGAALSR
                     DTAAQRFNVAASRARDRMILVRSVELDHLSESDRLRRGLIAHFARPFAEDPVRVTDLR
                     TLCESRVERDLFDWLNGEGYHVTPQVTVGAYRIDMVVEGRDDTRLAVECDGDKYQGPQ
                     QWVEDMRRQRTLQRVGWVFWRCFAGALLRRRQAILDDLRRTLAANGIEPTGQGSWGRR
                     RITETKRVRAPDSVTV"
     gene            complement(11816..>13004)
                     /locus_tag="VN496_14990"
     CDS             complement(11816..>13004)
                     /locus_tag="VN496_14990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HXF18311.1"
                     /translation="DEAEIRGHRRTYIGALPGNIIQAVRRAESRSGVLMLDEIDKLGA
                     GGFHGDPSSALLEVLDPEQNARFRDNYLGVDFDLSKIMFIATANVLDTIPGPLRDRME
                     IIQLPGYTAEEKLEIARRYLVRRQLEANGLKPEQVTLTDAAIRAIINDYTREAGVRNL
                     EREIGSVLRSAAMKIAEGKAQSVTLDAADLHAILGAPRFENEVAQRTSTPGVATGLAW
                     TPVGGDILFIEATRVPGSGKMILTGQLGDVMKESAQAALTLAKMYVGESLDKSDLHVH
                     VPAGATPKDGPSAGVAMFLALISLLAGKPVRSDVAMTGEISLRGLVLPIGGVKEKVLA
                     ALRAGIKTVMLPKRNEKDLEDVPSSAREKLEFVWLDNVEDAVRCAMGIEVKQLFAQQE
                     KAA"
BASE COUNT         2600 a         4011 c         3781 g         2612 t
ORIGIN      
        1 ctcccgatcc gatcatgtaa atacgcatgg ctcctccggt ctttttttat gttcaatcgt
       61 tctatactgc gagctccaag cttatcgcaa ttccgtccga tgccaacttt gcccgcgttc
      121 cagcgtatta gaaccaacgg ggtgaacctg cgctgtgcgg ttgaaggaaa gggaccgctg
      181 gtgctcatga ttcatggctg gccggagtcc tggtattcgt ggaggcacca gatcggaccg
      241 gtaagcgagg ccggttatcg cgtagtggtg ccggatgttc gcggctatgg cggcagcgac
      301 gcgcccgccg cgatcgaggc ttacgacatg gaaaacatga tcgcggatgt ccttggactc
      361 atcgatcatt tctcggagca gcaagccatt ctgtttggtc acgactgggg tgcgcccatc
      421 gtctggaata cgaccgcatt gcatgccgat cgcgttcgcg ccgtcgccgc tctctcggtc
      481 ccttatgcac ctcgcggcaa gatttcatct atcgagctct ggaagcagat ttacgctgga
      541 cgatttttct atcagctcta tttccagcag cccggcatcg cggaggctga attcgaagcc
      601 gacgtgcgaa cctcattgcg caagatctat tacgccggtt ccggcgattc gcccagaggc
      661 atgttccgcg ccgacaagcg gcccgacgcg aatatgctgg gcgacctacc ccatcccgcg
      721 acatttccgg actggctcac tgaacaagac ctcgactact acgcagcgga atttaaaaag
      781 agtggattcc gtggtccact gaaccgctat cgcaaccagc agcgcgattg gcaacggctg
      841 acggcactca gtggcgcacg catcagccgg ccagcctgct ttatcgccgg ccggcgcgat
      901 ggcgtgctca aatttgttcc ggattttgat atggtggaaa acatgaagcg ctggacggat
      961 gatcttcgca tctgcgaaat catggaaggc gccggacact ggatacaaca ggaacgaccg
     1021 gcccaggtca cgcaactgtt gctgtcgttc cttgccgggt taaaaaagga ctgagcgatg
     1081 aggccctgct tcgcaggata ccgggcgctg cccacaactg gtacaccttg cgcgatcccc
     1141 ttgggggcag tcctgtcttt caagactcgc tacaatgtcc acctgctaat cattcggtgg
     1201 attccatggc atcgagcaaa actgcaagtg ggctggagat agaggaactg gtcatcggcg
     1261 aaggtaacgt ggcagcctcc ggtcaaaacg tgactgtgca ttacacaggc tggcttacca
     1321 acggcagcaa attcgattcg agcaaggacc gcaacgatcc attcagattc ggcctcgggc
     1381 agggtcaggt tattcgcgga tgggatgagg gagtacaggg catgaaggtc ggcggcaaac
     1441 gcaaactgac cattccgccg gagctcggct acggcgcgcg cggagccggt ggtgtgatcc
     1501 cgccaaattc cacgttggtg ttcgaggtgg aactgctcgg cgtcaaataa tcgcacggcg
     1561 tgcgcctgcg acggttccgg ttcaagcggc ggcaagtcgg tctcgcacgc tttgccgcac
     1621 gagatttacg ttctggcgaa acgcgtaaag attgtcggtg taagcgagtg gcgtattaat
     1681 gcgattgact gcgcgttcaa tctcgttcag ccgcgcaagc agtgccctca attgctccgg
     1741 agtggattgg tcatccagct cgagctccaa ttccttgagt ttcgcatacc atcgatagat
     1801 gcgcgagcgc acgcgccagc gatagatagc aggaacgatg cggccaagcg gtatcagcaa
     1861 tgccagcagc ggcaacaaag tcagccataa gcggtcaacc atcacagccg cccaataagg
     1921 caaatagcgc tgtagaaacg gcggtcccga cttgtagtag cgctgcgctt cggcactgag
     1981 cggaaattcg gaattcatcg gcgcgggaaa ctcgcctgcc tttcgtaaca agcctgcgct
     2041 gccatggact tcactggcgg cgcgcatcag cagataagcc aaagcaggat gcaaactatc
     2101 cacggcaagc aggtttgcgg tggatgacaa aagtgtcacg tcgtgcgccg gcacattatt
     2161 cacgaaatca aacacgcctt gcggcaaagt cagtttcgtc agagacggaa aacggcgcgt
     2221 ataggcttca gcccgcttca ggtccagcag cctgatcccg ggtgccaggg cgagctgctc
     2281 cacgagcggt gattctggcg ggccaacgat gaaaacagcg tccagcttgc cccgcaacaa
     2341 catctcgttt gcctgcttgc cgtcatcggg caacagcatc gtgggcggca tcacggcagc
     2401 gtttaccgcc agcaactgca acgccagcgc gcgactgccg cttgcttcag gaccgatggc
     2461 gagttttttg ccgcgcaatc cgccgaaatc gtgcacttcg ggtccgcggt agaaaaccca
     2521 taacggctca taataaacac tccccagcga cacaagttcg ggggcatttg cggcgtacgc
     2581 ggagccgccc tgcagaaatc cgacttcgac cgcggattga tcatccagca gccggccgat
     2641 gttctcctgc gagccggccg aggaccgcag ttcgagatgg acatcgtcgc gcgccaggat
     2701 atctcgatag cgcagggcaa acatgtgata accgctgtct acgggaccgg tcgaaagcac
     2761 gattgaatcc ggaggggccg gttccaggaa acgcaacgcc accacgaata cgcccagcac
     2821 cagcagcgct acaggaatga cagtcagcca gagatcacgg cggctcaggt cgcggatttt
     2881 ggcctcaaac attttttggt tacgcaacag cgggcgcaac gcccatcgcg cttgatagag
     2941 agcaaaattg tgtgcgggag ggcaccaagt ctacacgtca aacaacagag cgtgccgatg
     3001 gtcgatattc acgggtcggg agacgccagc taagtgcctg cagtccgctg caaaactgca
     3061 cgcacggcac tcggaagcaa caccaaatac gcaaagagga tgcggcgggc aagaagcagc
     3121 tcgaccgctc tggaatgata caagcccaaa acgccgtgtt gcccagtcgg accttacgct
     3181 aaccgacccc gcgccggtcg ttttcttcat cgatttcttc aaccaggccc tggaagcgtt
     3241 cgatgacccg ctccacaatc cattcaggtt cctgcgccca gaagttcact accgaggaat
     3301 cgtcgtcgga ggccgcgtcg gtgcctgtca cagtgacatc ctcttcctcc tgcaggatat
     3361 agctgagcgc ggccatgact tgcggataat gaatggacac cgcatctccg gcggtggcgc
     3421 cctcatccat ctcgccacga atcagggttg cggcttgttc gagtaccggg ccggcgcccg
     3481 ccgcctttag cgcatccggt tcactggcga ccagacgctc aagcaagtcc aggatgaccc
     3541 gttcctgcgc aaagatggcc cctgccacga attcggcata ggtcaggtca attgacgcca
     3601 ccaggtggtc acattctcca gccagcattt cgcaaatcgg acagcgcccg ccagcgacaa
     3661 cttcttcctc ttcgtccgct tcatcgtcct tggctggatc gtccaggaaa tcatcctcag
     3721 gattacgtgg agtgggcata gtctgtctcc ctgttaagcg agccgagctg ggaccggaca
     3781 gaagccggat ctcccagtgg cgctggcaac ggcggcgtgt ctatcggagg cggttcgcgt
     3841 accggcctcg gatcgtccgt ggtcggcggt ccgacgggcg gattgtcgtg ccgcggatct
     3901 cccggcggca actcgggtct cggtctgggc ataatagtct gcagaccagc cggtggcgtt
     3961 caagttcgcg cgcactagcc ggattgagcg gccggaattc taagtctgga ttgacggacc
     4021 agctggtctt ttgcctgcct ggtgagctgc tttatctcgt attcgcgttt cgctgccacg
     4081 gaacgcgacc gcgccggttc cgtgtacacc agtaccacgg gacggcgact tcgcgtgtaa
     4141 tgcgcaccca gcatgccgct tgaattatgt tccgcaagcc gccgggccac gtccttggca
     4201 atgccggtat acaggctctg atccgcacag cgaaccatat agacaaacca catggcgtaa
     4261 gggggagcga ctcagggtcc cggggcaaca gccgggaaac gcgcatagcc gaagctcgag
     4321 aatttcgccg ccatcatggt tctggccggc cttttgtcct ggctacttca ctttttagtt
     4381 cctccaccgc gcggcgcagc gcggccactt cgtcgcgtag aacagccact tcgccctgaa
     4441 gcgccgttcg gctttcgtcg cccgctgcct cgagggcgac agccgaactt gcgttctgcg
     4501 catccacggg accacccagt aaatgcgccc agcgatgctc ccgtgagccc ggttgtttcg
     4561 caagtttgac caccaatggt ccattcggac gcgaaaccat ctcactcaaa taagcgtcaa
     4621 cgctagacag atctgcgaag cgataaagcc gctcgcatgc cgtacgcatc tcgcctgtgg
     4681 tctgcgctcc tcgcagcatc agagtaacga gcaacgcggt ggtgcccgag tcggtaccga
     4741 gcacttgagg aagattatga gaatagcgca ccaccctgcg ggacgcgccc cagctgttag
     4801 tgaccagctt gcgttccttc aggctatcca gtgcctcttg tacctctgcc tcgctggctt
     4861 ccatgaccgg atcacggctg gtcttttggt tacaccctgc taccagccca ttcagggaca
     4921 gcgggtagta gtccggcgtc gtcaactgtt tttctgccag cacccccaga acgcggattt
     4981 ccaatggagt cagcaacggc acgttcgagt cagtcatgtc gcggagcctt gggacgcgga
     5041 agcagaaatt tacacgagaa aagcagccag cagtgcctta gccagccatg cccgcatgaa
     5101 tcctcatccc gcgtgggcgt tccaatgaac actgaaaact gtgccccccc ggggagtccc
     5161 ggagtttgca tcgatcgccg gctcttcatc aaaatggcag ccgcgctggc ctgcacgccg
     5221 ctgcttgatc gagtccatgc gagcggcctt gtgcgccctg ccgcagtccc cggcggtttt
     5281 gctgtagtac agcttggtcc ggcccgcatg gcgccggtgg tgcatttcaa tggcgaccgc
     5341 gtattcgttt ccggaacgca aaccggttgg ttcgccattg tcggcattcc acttgacgcg
     5401 cagccgggcc gcgccgctcc tctcttcatt gaagatcctg ccggcaaatc gcgcgtcact
     5461 tatttcacga taggcgtaaa gcattacgaa acggaatacc tcagcatgag atccgattat
     5521 gtcgatctcg agccagaaaa ccttgcccgc tgcgaaaccg agcgatcgca cctgcaacgc
     5581 gtgctgcgaa cctattcgga cgcgtcgcct tcctcgttgc tgctggttcc cccctgccaa
     5641 ggaacacgca ccagcacgtt tggcctagtt cgctttttca atggacagtc aaggggcgcg
     5701 cacaacggca tggatatcgc ggcgccagcc ggcaagccgg tattcgccgc ggcatctggc
     5761 gaagtcgtcg atgccggaga ttatttcttt tctggacgaa cggtcatcgt cagtcacggc
     5821 cgcgggttca ttaccctgta cgctcacctg agcgaagtca aggtcagagc ccgcaagcgc
     5881 gttaccgcgg gtcagaagat cggcaaaatc ggcatcaccg ggagagccac cggaccgcat
     5941 ttgcacttcg gcgtgtatct gaatgcagct gccgtggacc cggccctttt cctgctcacc
     6001 gacccacgca cgcaacacct gtattcgccg agcaggcggg cgcccaattc tggagaaccg
     6061 tttggcgcac cgttgcggga aacggtcatc aaacaactct aacctgccga gcaagtttgg
     6121 aagcggagtg cgcgcccggc ttgcatcccc ggtatgatcg cgcaatacaa tcctgacaaa
     6181 ttcgaatccg tgcttcggcg ccatttcctg caactcgtta cggccgtctc gtcgctgtgg
     6241 atattccacg cgcaagcggg ttcactgcag aaagccgcgg cggtaccggg aggagtggcc
     6301 gttgtcaaaa tcggctccgc tccgaagcca ccgacggccc gggtcaatgg caacagggtg
     6361 ttggtcactg gcagtgaaac cgaatggctt gcagtcgtgg gcattgcgct cgaagcccgg
     6421 gctggcgaca aactggcact cgcagtggag tttgctgacg gcaagaccgc gactctgcaa
     6481 gttgcgattg gaacgaagca atatgcgacg cagcgcctga cggtcaagcc gggtcaagtg
     6541 gaactttctc cagaggacct ggcgcgctac gaaaccgagc gcatgcatct tggagaaatc
     6601 cgcaagacct ttaccgaagc cgcacccgat tcgctggagc tcatgcagcc gtgcgaaggg
     6661 ccgcgtgcca acacgttcgg ccagcgccgg atcttcaacg gccaatcgcg caatccgcac
     6721 aacggaatgg acattgctgc ggcggatggc tcacccgtgt tggctgcggg tgaagcgaag
     6781 gttatcgacg tggggaatta ttttttctcc ggaaacaccg tcatcctgga ccacggcagc
     6841 ggcctactta cgctctatgc gcacttaagt tcggtagaag tcgctccggg ggaccgggtc
     6901 ggcacgggag cgcgcattgg gcgcgtcggc gccactggcc gtgtcaccgg ccctcacctg
     6961 catttcagcg tttttctgaa ttcggtggcc gtcgatccgg ggatttttct ggcagacatg
     7021 tagcagttgc tgctcgcgtt cgtgtccgca actcacacgg tcacgctatc gggcgcccgc
     7081 acgcgtttcg tttcggttat gcgtctgcgt ccccagctgc cctgcccggt aggctcaatg
     7141 ccattggccg ccagcgtgcg gcgcaggtca tcgagtatgg cctgacggcg gcgcaatagc
     7201 gccccggcga agcaacgcca aaacacccat cccacgcgct gcagcgtgcg ctggcgccgc
     7261 atatcctcca cccactgttg cggtccctgg tatttgtccc catcacactc gactgccaga
     7321 cgggtgtcat cgcggccttc caccaccata tcgatccgat atgctccgac ggtaacctgg
     7381 ggcgtcacgt ggtaaccctc accgttgagc cagtcgaaca agtcccgctc gacgcgcgat
     7441 tcgcacagcg tgcgaaggtc ggtaacccgg accgggtctt ccgcgaatgg ccgggcgaaa
     7501 tgggcgatga gtccgcggcg cagccggtcc gattcggaaa gatgatcaag ctccaccgag
     7561 cgcaccagaa tcatgcgatc gcgcgcacgc gacgcggcaa cattgaagcg ctgtgcggcc
     7621 gtgtcgcggg acaatgcagc cccgatgtcg ttcggcgcgc acaccatcga caggtacatg
     7681 atgtcgcgct cgcggccctg gaattggcgc gcatcaccgc acgtaaccgc gtgcctgcgt
     7741 agcgtatcgg ggctcagcac ttcggtcagg cgctgccaga tttgcagagc ctgttcttcg
     7801 cccagcaggg aaacaatgcc aatcgagcgc gactgcattt ttggatcagc cacgaaggcc
     7861 ctgacgtcct tgacgatgaa ttcgatttcc gcccggttga tggcgccctc gcgacgcgcg
     7921 tcgccgacga gataatcgag caggggcgga tcgagccgct cggaagagtt ggcaagccgc
     7981 aagggataca gttcatgctc atagaattcg cgcttggaat actcgatgat cggcgcgaca
     8041 caacgaaaat gttccttcaa cattacgccg ctgtgcgcga acaccacctt ggcaaggtcg
     8101 taaatggagc gctccggtga catctgcgcg gcatacaaag gaacctgttc cgctagatag
     8161 cgggtcatca aagtattaat gcgttcttcc tcgatgccta ccgtctgcgg agcaacctgc
     8221 ttatcgtctc cgacgacaag aatttttcgc gcgcgcagga ggaccggcaa tgcagaaaga
     8281 tcggattgcg atgcttcgtc aatgattacg agatcgaagc aaccgagttg cgccggcagc
     8341 gattctgaaa cgcggtgatg cggcatgatc cagcaaggca ccgcgtgctg tgcctgcgcc
     8401 gacgcggccc gcgcatctcg gcgatagcga aaggcgcgtt tgccggtgcc tttgccgagg
     8461 tgctggatgg cgttcagata ggcctgcaat gcggcacgca cgtcaggagt ggcattttcg
     8521 gcaagtttca gccagctgcg ctttacgacc aagtcttcat aagcgcgcgc cagatcgagt
     8581 tccacttcca cgcgcgcagc ggaaagcttt ttcagctcgt catgcgcatc gatcgcctgc
     8641 aaatgcgtcg ccaatcgtcg caggcgccag tcccgcagca tggtggcggt agacagcttg
     8701 tcgtcgaaac cggtacccat cagccgcagt ttctgcgcga gctttggtgc gccggacgcc
     8761 gcgatattgg ccgtaacgac cgtcaccacg gcaagcggcg cggccagcga tcgcaatctg
     8821 gaaagttcgg cgcccagcat attccacgct tccagcagcg cggattcatc gacagccgga
     8881 ttgccaaggg tgccggccag aaattcgcgc atgcgactga tgatttttcc ctgcttgcct
     8941 tccagcgcgc gatgcagacc gttcaccaca gtggagacct ggctcagttg ggattttgtc
     9001 aggtggtacg caagcgcgag ctccacttcg gacagcaccg cgctattctg atgggccgta
     9061 gctgcgcgcg cccacgcggg gaacagttcc attgccatgc gcgacaactg cgcttcctcg
     9121 cgcgccagca gcttcagttt ttcatagtgc acgagttgcg attctgcgga cagtcttccc
     9181 ctggcgtcgg ccgaaagaac ggtctggaat ccaatgtccg tggctagcgc attccatcgc
     9241 gcagtcaggg cacgccttgt tttctgcaac tcgacatgtc caaggacggc cttccaatcc
     9301 gccatgtcag caggccgcga accgttgacg cggaccatgt cgagcagttt tttcgcctcg
     9361 ggtcgaccca acccgattag accgaacggg cgccttcctt ccgcaagatt gatgactgcc
     9421 tgatgaaatt gcgcgtccgt gtccgcggcc tcgggcacga ttaccggtcg agcgagcagc
     9481 tgggcgcgct gtgcggcggc gcggtccatg tccgcagcaa gtgcgtccag caaatcgaac
     9541 aaccgtttcg gctccccact gcgcatacgc gcgatcgctt cttcgctcca tggcagtcgc
     9601 aatcccgtca gttcgtcgcg caaggattta acgcgcgcga tctgttcggc gagtgcacga
     9661 gcccccagca gggattcggt acccgagccg gccggtaccg caagttcgct tgagcgtgcc
     9721 tcgcgcgtga cttttgcata gcgttgcaga tcatcatgcg cgcgtaccaa tgccagagcg
     9781 tccggaaaat cctccgctgc cggcaacgca ctgccggcgt actggatatc ctcccccagc
     9841 tgacggcgcg cttcgcgcag ccgcgcaata tcttccgcgg aaaaccgggc aatgtactgc
     9901 ggccccacgc ccagcccgtc cggaatccat tcgtactgtc ctgcgccctg catgacttcg
     9961 cgggcggcat cctgcgggtc aattgtttct ccgccaagat cgatgcgcga aacattgagc
    10021 ttggcccagc ggcccaaatc tgtatcgatg cgcttcagct tgccatggaa gccatcgatc
    10081 atttccacca gaccctccac ctgccgtgct gcggtgaccg tatcgaggct ttgcacttct
    10141 gccgctattt ttcggatcga gcgctcgaac tggtcgaccc catcctggtc ggcggacagc
    10201 agcggaatcg ccagtggtcg aatttcgtga ggcaagagct cacgtaacac cgccagcgcc
    10261 gggtcgcgca tggaggtcac cagcacgcgg cgtccgttgg caagccaatg gcagatgatg
    10321 ttcgcgatcg tgtgcgtctt gcccgtgccg ggcggacctt gcaccaccac accatcggaa
    10381 atctcgagca tctgaactat gcgcgcctgc tcatcgttga acggcttggg gaagaacagg
    10441 tcttgcgcat cggtactctg cgccgccccg gcgtggtatg cggcggttac tccgcgatac
    10501 gcgggcagcg cggcaggtga gaccaggctc gacggttctg tcaccaaagc tgcaacggct
    10561 tgcggcaacg agctctcatt gtcgagcaat gaaagctggt cgcgaaaacg ctcgaggtcc
    10621 tgtacggcgg caacgctgct gcgcggtcgc gcgaacaata cccagctact gctgatttcg
    10681 cactggtttg gttcaccggt ttgctgtgcc gccgattcga atgtgcgacg ggcaaggtcg
    10741 agcaacggtt cgatgctcgc aggtgcgaag ggcgacaaag tatcccgcgt cgttatcagg
    10801 aactgctcgg cttctttttc cagattcgac caggcgggag tatcggcgtg aaaaaaaaca
    10861 tcaatttcca cccttggcgc aatatcgcgg gggcgtaact ccgctgcctg cgtttcagga
    10921 ttgaaactca gttccatcgt ctgcgtgatc agcggatagc tgattgccga gcccgctttg
    10981 cgggcagtac acaaagccat gccccagacc aattccagct ggccctccac cagcgcgccg
    11041 gccagctcct gatgcagcgt aaaaagaccg acgtagagat ttgcaaggcg ccggcgctgc
    11101 gattccgcgt ccgcccaggg ccgccatgag gcctccagat aacgtgcata ctgcgcctcg
    11161 acttcgttgc gaaattcgta atcctggaag ctcacgcaca catgcgaatc cacggccggg
    11221 gaagcggcat ccggcccagt gatatcacga tgagttcctg cggtaatcag cgccgcgccg
    11281 tcgatctccg tcgcgagttg cggggccgcg ctcaccgtgg caccaacatt cagccagggc
    11341 gaaagccaag ggctttctgc ctgcggcggg acttgcgggc ccggtggatg aggtacccgc
    11401 agccacgtct cgtcgtctgc atccatgcgc cgaccttcaa gcaggccggg aatttcgcgg
    11461 gcttgtggct cgtacagcag gaaactatcg tggctggtga cgtcggacac cacgcgaccg
    11521 cgcgtgcgca acgaatgttg tgcaaagtct acgagcgcga cgaggcgccg ttgctgaatg
    11581 gtcacggccg ttgctacggt tcttgtatta cgttctgtgt ttctgcgtgt ggcgctatcg
    11641 tactgcactc aaggcgcaat cgatagcacc acgcgcaaaa gcagcgtgca cgcgcttgcg
    11701 cgttgaacgg cgcatcaaga gactatgcgg cgtgaaggac ttagctggcg cgccgcaagt
    11761 gaatgcttaa aaaacaggac tgagcgatcg ctcagtcctg tttcttgtat tgcttttatg
    11821 cggccttttc ctgctgtgcg aatagttgtt tcacttcaat gcccatcgcg cagcgcacgg
    11881 cgtcttcgac gttgtccaac catacgaact cgagcttctc gcgggcgctg gacggaacat
    11941 cttccagatc tttttcgttg cgcttgggca gcatcacggt cttgattccg gctcgcagcg
    12001 cggccagcac tttttccttg acaccaccga ttggcaacac gagaccgcgc agcgatatct
    12061 cgccagtcat ggcaacatcg gaacgcaccg gtttgccagc gagcagggaa atcaacgcca
    12121 ggaacatggc tacgccggca ctcggaccgt ctttcggcgt ggcgcccgcc ggaacgtgca
    12181 catgcagatc cgacttgtcc agcgactcgc ccacgtacat ctttgccagg gtgagcgccg
    12241 cttgggccga ttccttcatc acgtcgccga gctgcccggt caggatcatc ttgccggaac
    12301 ctggcacgcg ggtcgcctcg atgaacagga tgtcaccacc gaccggcgtc cacgccagcc
    12361 cggttgccac gcccggtgtg gaagtgcgtt gcgctacttc gttctcgaat ctgggggccc
    12421 ccagaatggc atgcaaatcc gccgcgtcca aggtcacgct ctgcgctttt ccctcggcga
    12481 ttttcatggc tgcggagcgc aggaccgagc cgatctcccg ctcgagatta cgcacgccgg
    12541 cttcgcgcgt gtaatcgttg ataatggcgc ggatggcagc atccgtaagc gtcacctgtt
    12601 ctggcttcag cccgtttgct tcgagttgac gcctgaccag ataacgccgt gcgatctcga
    12661 gtttttcctc ggccgtatag cccggcagct ggatgatttc catacgatcg cgcagcggtc
    12721 ccggtatggt gtcgagcaca ttggcggtag caatgaacat gattttcgac aagtcgaagt
    12781 ccacacccag atagttgtcg cggaacctcg cgttctgctc cggatcgagt acttccagca
    12841 acgcgctgga cggatcgcca tgaaagccgc ctgcacccaa cttgtcgatt tcgtcgagca
    12901 tcagcacgcc gctgcgcgat tccgcgcggc gcactgcctg gatgatgttg cccggcaagg
    12961 caccgatata cgtgcgccga tgaccgcgaa tctccgcttc atcg
//
LOCUS       DATMZQ010000471         7360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4397984, whole genome shotgun sequence.
ACCESSION   DATMZQ010000471 DATMZQ010000000
VERSION     DATMZQ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4397984"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VN496_14995"
     CDS             complement(<1..479)
                     /locus_tag="VN496_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18312.1"
                     /translation="MTKQFSTATHCVAALIVLGFTQPILADDHEFEAPLVACLSNGTA
                     IGGVNSCGKIWKLKSGKAELKAGKLEVEVKGLVLNDASVGQYNGSPDGVDAVAAAVIC
                     HGPSGASVAAQLEPVALSKTGDAKVEGKVSLPKGCVGPVIVLRERYEGKIGGWLAGTG
                     "
     gene            734..1150
                     /locus_tag="VN496_15000"
     CDS             734..1150
                     /locus_tag="VN496_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF18313.1"
                     /translation="MPVMPFHLAFPVTSLDKARAFYGGLLGCPEGRSSAEWVDFDFHG
                     HQIVAHLSPEAGHRDSNRVDGDNVPVRHFGLVLPMVAWQKLADKLTAAGVKFIIEPHI
                     RFKGQPGEQATMFFLDPCGNAIEFKAFDNPAMLFAK"
     gene            1828..4248
                     /locus_tag="VN496_15005"
     CDS             1828..4248
                     /locus_tag="VN496_15005"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HXF18314.1"
                     /translation="MQGKFPALATRRDYYMALAYLVRDQLLHRWVSTAEAYTRQQSRT
                     VAYLSAEFLLGPHLGNNLVNLGIYAQVEQAVAELGLDLGELLEQEEEPGLGNGGLGRL
                     AACFLDSLATLEIPAMAYGIRYEFGIFEQEIVDGSQVEKTDKWLRFGTPWDIARPEWA
                     VEVRLGGRTETYTDVQGRPRSRWLPAQTVIGVPYDTPILGYHTNTANTLRLWRAEAPE
                     SFDFAVFNRGDYYGAVDRKIVSENITKVLYPNDEAVRGKELRLEQQFFFVSCALQDML
                     RIMRGQKIPLQRFHEKFAVQLNDTHPAIAVAELMRLLVDENAMEWDQAWAVSCNTFAY
                     TNHTLLPEALERWPLGLFGKMLPRHLEIIYEINARFLDEARIRFLGDEARIARMSLID
                     ESGERYVRMAHLACVGSHAINGVAGLHSELLKQDVLADFHMLWPDKFSNKTNGVTPRR
                     WLALSNPKLAQLITDAIGDSWVKDLAQLRQLERLAEDGQFRSKWRDVKQDNKRQFAAL
                     MHGKTGVTIDPDSMFDVQVKRIHEYKRQHLNVLNVIAHYLRLKAGQAADEPPRTYIFG
                     GKAAPGYFMAKLIIRFINAVGDVLNRDPAAREHMRVVFLPNFNVSSGQRVYPAADLSE
                     QISTAGKEASGTGNMKFQMNGALTIGTLDGANIEIREEVGLENFFLFGMTTEEVAQRW
                     VTGYRPADCYEADAELREVIDLVRAGFFSRGDTELFRPLLDNLLLHDPYLVLADFRAY
                     VDCQREAGRAYADRQQWSRRSILNTARSGKFSSDRTIREYSDEIWRVKSVGIKLLSRD
                     DVKGKFLQ"
     gene            complement(4297..4971)
                     /locus_tag="VN496_15010"
     CDS             complement(4297..4971)
                     /locus_tag="VN496_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXF18315.1"
                     /translation="MFLELQKFDTPTIANAVEIAQGKRSINGFTRQTLIAASPKLPSI
                     VGYAKTARIRCSTPYDPAQRRKNQVAYYEHVAQGERPTIAVVQDIDEQPGIGAFWGEV
                     NTHVHWGLGCIGAVTNGSMRDLAMMHPEFRCLAGCLSPSHAWVQVVDFGGPVEVFGMT
                     VTDGDIVHADLHGAIVIPRDALAKMPAAIDLMARREKVILDAAKAPGFGMAALRDALA
                     ASDQIK"
     gene            complement(5285..6445)
                     /locus_tag="VN496_15015"
     CDS             complement(5285..6445)
                     /locus_tag="VN496_15015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXF18316.1"
                     /translation="MDFTPTPEQLAITEGIAKVCAKFDAQYWLERDRDGGFPEDFYRA
                     VAQGGWLGIAMPSEYGGAGLGITEAALMMQAIAESGAGFSGASAVHMNIFGLNPVVVF
                     GTTEQKGRWLPPMIRGEQKACFAVTEPDAGLNTASITTAAQRKGDQYIVRGRKIWIST
                     AQVADKMLILARTSSGDAKPTDGLSLFYTDLDRSFVEAREIDKMGRKAVDSNLLFIED
                     LPVPASDRIGAEGSGFEYILHGLNPERILIAAEAIGLGRAALRRGVAYAKERIVFDRP
                     IGQNQAIQHPLARCWMELEAAHLMMCKAAALYDKGEACGAESNAAKYLAAEACYRACE
                     QALLTHGGMGYAREYHIERYLREAMIPRLAPVSPQLILCFIAEKVLGLPKSY"
     gene            complement(6473..>7360)
                     /locus_tag="VN496_15020"
     CDS             complement(6473..>7360)
                     /locus_tag="VN496_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXF18317.1"
                     /translation="MARLGLSYEELSALNPEILYVGAYGYSQNGPYAAKAAYDDLIQG
                     ISGVPWLVAQTGAAPNYAPVNLADRLTGLHAVYAVTAALFQRSRTGRGESIEVPMFES
                     VAHFVLGDHLAGFTWDPAEGPGGYQRLLARKPYPTSDGYLCVLVYNDKQWKSFASVIG
                     RPELLQDSRFATQASRAANIRDIYAFLTQLFRTRTTAEWMALLESVDIPVARMNSIED
                     VVQDHHLLKSGFFTIEDHPSEGELRAMRTPTNWSESQPAVPRPAPCLGEHSAEVLREA
                     GYSEVEIAELVRKGVTKVA"
BASE COUNT         1477 a         2189 c         2156 g         1538 t
ORIGIN      
        1 ccggtaccag ccagccagcc gccgatcttg ccctcgtaac gctcgcgcaa aacaatcacg
       61 gggccgacgc aacccttggg tagcgatact ttgccttcga ccttcgcatc gccagtcttg
      121 ctgagcgcta ccggctccag ctgtgccgcg acgctcgccc cggacggacc atggcaaatc
      181 accgccgccg ccacggcatc gactccatcc ggcgaaccgt tgtactgccc gaccgaggcg
      241 tcgttcagca ccaggccttt gacctcgacc tcaagcttgc cggctttcaa ttcggctttg
      301 ccggatttca gcttccagat ttttccgcag gaattgaccc cgccaatggc cgtgccattg
      361 gacaaacagg ctaccagcgg cgcttcgaac tcatggtcat cggcaagaat gggctgcgtg
      421 aaccccagca ctataagggc tgccacgcaa tgtgtggcgg tagagaattg cttggtcatg
      481 actcaaattt cctttctttt tttgttgact gttattgaac agcggaagtg ctccctggat
      541 ctcctgcggg tgtgcgcgag ccatgatcgt ccagcattcg ctcaagcacc cagcgtcata
      601 gtatacgcac acgggattac acatcagtta cgccagcaat cgccgatgtg gtgtcggcgc
      661 aatcgagcca tcgccaccga tcacggtgcc gccgtactgt aaactgtgat cgcatttttt
      721 caggagcagc ttcatgccgg tcatgccgtt tcatctggcc tttcccgtca ccagtctgga
      781 caaggcgcga gccttctatg gcgggctgct gggttgcccg gaaggccgct cctccgcgga
      841 gtgggtcgac ttcgattttc atggacacca gatcgtcgcg catctttccc ccgaggcggg
      901 acatcgggac agcaataggg ttgatggcga caacgtgccg gtgcgccatt tcggtctggt
      961 gctgccgatg gtggcctggc aaaagctggc cgacaagctc actgcggcgg gcgtcaagtt
     1021 catcatcgag cctcacatcc gcttcaaggg ccagcccggc gagcaagcga ccatgttttt
     1081 cctcgatccc tgcggcaatg cgatcgagtt caaggcattc gacaatcctg cgatgctgtt
     1141 cgccaagtga gctgcgggtg tgtgccgtcc gcacctagac cggaatcatg ccgcgatttg
     1201 cgaggcccaa gccgttagcc gtgtgcagcg aatgtcgtgc gattaccgat ttgcatgaac
     1261 aggtgaatca tcgctgcagc cggaccgtga atggccggcg ttgcgcgggc atttatcaca
     1321 gcaatctttc cttcgtgtgg gataaatgtc agccttgcaa ggcgacaggt cgtatcggaa
     1381 gacaggtctg tgtcgaatgc aatggcatgg ggtggcattt gcacgactga gttgttcacg
     1441 atcggcaagc gagcaacgtc tttcagtgca acttcgacaa acgtccttaa tccataccgg
     1501 cacattcgca gtgcaccgtc cgcgtgcatc agcgaatgaa attgtgcggc gacggtgcat
     1561 gcgcacgagc tgccgtgtta gcgcaggttc atacccacca agggccccgc cggacgtggc
     1621 tctcgcgagt gtctctcgcg ggtggcactg gcccggcgca tgcggaagcg ccgcgcatgg
     1681 tttttgcggc gaggactgtg caacgccaac agcggggagg aagaaatgaa agcaggcgtg
     1741 acgaaaatcg gggcggcact taaagaagaa catagccggc cgggcaagga aacgctgaag
     1801 cgcgaattcc tggacagcct gttctacatg cagggcaagt ttcctgcgct cgccacccgg
     1861 cgagactact acatggcact ggcttacctg gtgcgtgacc agttgctgca tcgctgggtc
     1921 agcactgcag aggcttacac acggcagcaa tctcgtaccg tcgcgtatct ctcggcagaa
     1981 tttctgctgg gtcctcatct gggcaacaac ctggtcaact tgggcatata cgcgcaggtc
     2041 gagcaggctg ttgcagaact gggactcgac ctgggtgaat tgctggagca ggaagaagag
     2101 cctggacttg gcaatggcgg cctcggccgt ttggccgctt gctttctcga ttccctggcg
     2161 acgctcgaaa ttccggcaat ggcctatggc atccgttatg agttcgggat cttcgaacag
     2221 gaaatcgtcg acggcagcca ggtagaaaaa accgacaagt ggttgcggtt cggcacgccc
     2281 tgggatatcg cgcgtccgga atgggccgtg gaagttcgtc tcggtgggcg caccgagacg
     2341 tatacggatg tgcagggtcg gccgcgatcg cgttggttgc cggcgcagac tgtgatcggc
     2401 gtaccctacg acacgccgat cctcggctat cacaccaaca cggcaaacac cttgcgcttg
     2461 tggcgcgccg aagcacccga gtccttcgat ttcgcagtgt tcaatcgcgg ggactattac
     2521 ggcgcagtgg accgcaagat cgtctcggag aacatcacca aggtgctcta tccgaacgat
     2581 gaagcggtaa ggggcaagga gctgcgcctt gaacagcagt ttttcttcgt ttcgtgcgcc
     2641 ttgcaggaca tgctgcgcat catgcgcggg caaaagatcc cgctgcagcg cttccacgaa
     2701 aagttcgccg tacagctaaa tgacacccat cctgcgattg cagtggccga gctcatgcgc
     2761 ctgctggtgg acgagaacgc catggaatgg gaccaggcct gggcggtatc gtgcaacaca
     2821 tttgcctaca ccaaccacac gcttctgcca gaagcgctcg agcggtggcc gctgggactg
     2881 ttcggcaaaa tgctgccgcg gcatctggag atcatctatg aaatcaacgc gcgttttctg
     2941 gacgaagcca ggatccgctt cctcggcgac gaggcacgca tcgctcgcat gtcgctgatc
     3001 gatgaaagcg gtgagcgtta cgtgcgcatg gcccaccttg cgtgcgtcgg cagccatgcc
     3061 atcaacggcg tcgcgggcct gcattcggaa ctgctgaagc aggatgtgct ggctgatttc
     3121 catatgcttt ggcccgacaa gttcagcaac aagaccaatg gcgtgacacc gcgccgctgg
     3181 ctggccttgt ccaacccgaa gctcgcgcaa ctcattaccg atgcgattgg agacagctgg
     3241 gtcaaggacc tcgcgcaact gcgccagctg gagcgccttg ccgaagacgg tcagttccgc
     3301 agcaaatggc gcgacgtcaa gcaggacaac aaacgccagt tcgccgcgtt gatgcacggc
     3361 aagaccggtg tgacgatcga tcccgattcg atgttcgacg tgcaagtcaa gcgcatccat
     3421 gagtacaaac gccagcacct gaatgtgctg aacgtcatcg cgcattattt gcggctcaag
     3481 gccggacagg cagccgatga gccgccgcgc acttacatct tcggcggcaa ggccgcaccc
     3541 ggttatttca tggccaaact gatcatccgt ttcatcaatg ccgtgggcga cgtgctgaat
     3601 cgggatccag ccgctcgcga gcacatgcgc gtggtgttcc tgccgaattt caacgtcagc
     3661 agcgggcagc gcgtttatcc ggctgccgat ctttccgagc aaatctccac tgccggcaag
     3721 gaagcgtcag gaacaggcaa tatgaagttc cagatgaacg gcgcgctaac cattggcacg
     3781 ctcgacggcg cgaacattga aatccgtgaa gaagtcgggc tagaaaattt tttcctgttt
     3841 ggcatgacga ccgaggaagt ggcgcagcga tgggtcactg gctaccgtcc tgcagattgt
     3901 tatgaagccg acgcggagct gcgcgaagtg atcgacctcg tgagagcagg ttttttttcg
     3961 cgcggcgata cggaactgtt tcgcccgttg ctcgataatc tgctgctgca tgacccttat
     4021 ctcgtgctgg cggatttcag ggcttacgtg gactgccaac gcgaagccgg acgcgcctac
     4081 gcagaccggc aacagtggtc gcgcaggtca atcctgaata ccgcgcgcag cggcaagttt
     4141 tcctccgacc gcactattcg cgaatacagc gacgaaattt ggcgcgtaaa gtcagttggg
     4201 atcaaactac tgtcccgcga cgatgtgaag ggcaagtttt tgcagtagcg cgttgaagat
     4261 ccgacccgcg atgggtcgag cgtcgttctg cgtttgctac ttgatttgat cggaagcagc
     4321 gagggcgtcg cgcaatgcgg ccatgccgaa gcccggggct tttgccgcat ccagaataac
     4381 cttctcccgc cgtgccatca ggtcaattgc cgccggcatt tttgccagtg cgtcgcgcgg
     4441 gatgacgatt gccccatgca ggtccgcatg cacgatgtcg ccgtccgtca ccgtcatgcc
     4501 gaaaacctct accgggccac cgaagtcgac cacctgcacc caggcatggc tcggtgacag
     4561 gcagcccgcg aggcaccgga attccggatg catcatggcg agatcgcgca tcgacccgtt
     4621 ggtgactgcg ccgatacacc ccagtcccca atgcacatgg gtgttcactt cgccccagaa
     4681 agcgccgatc ccaggttgtt cgtcgatgtc ctgaacaacc gcgatggtcg gccgttcgcc
     4741 ctgcgccaca tgctcataat aggcaacctg atttttacgc cgctgtgcag gatcgtaagg
     4801 cgtcgaacaa cgaatccttg ctgtttttgc gtagccgacg atggatggca atttcggtga
     4861 cgccgcgatc agcgtctgcc gcgtgaagcc attgatcgaa cgcttgccct gcgcgatttc
     4921 cacggcattg gcgatggtcg gggtgtcgaa cttctgcaat tccaggaaca attcgcgagg
     4981 ggtcattgcc attctcttca gagttggggg ggttgataca actgtgtacg ggacggcaag
     5041 tttatcgccc gcgccgacat catgcttgtg tatagaattc cggcgcgcga ccgttgtaaa
     5101 ctgcggggca gtgtggcgct tccaacagcc ctgctgttat tcgtttcggc agtctgctca
     5161 acgggcgctg ccggaccgga tggagatgac acacccatgc tgcttcatct gacgttatct
     5221 cgattggcgc cctcattgtg atcatcaatt cggcaatggt attgcttggc cgctattgat
     5281 cccttcagta agacttgggc aggccaagaa ccttttcggc gatgaagcac agaatgagct
     5341 gtggactgac gggcgccagc cgcgggatca tggcttcgcg cagatagcgt tcgatatgat
     5401 attccctggc ataccccatg cccccatgcg tgagcaacgc ttgttcacat gcgcggtagc
     5461 aggcctcggc cgcgaggtat ttggcggcgt tcgattccgc accacaggct tctcccttat
     5521 cgtatagcgc ggctgccttg cacatcatga gatgcgctgc ttcgagttcc atccagcagc
     5581 gcgccagtgg atgctggatc gcctggtttt gaccgattgg ccgatcgaac acaatgcgct
     5641 ctttcgcata agccaccccg cgcctgaggg ccgcgcgccc tagcccgatt gcttcggcgg
     5701 cgatcaggat gcgttccgga ttcagcccgt gcaggatgta ctcgaagccg ctgccttccg
     5761 cgccaatgcg atcggatgcc ggaacgggca gatcctcgat aaagagcaga ttcgaatcca
     5821 ccgccttgcg gcccatcttg tcgatctcgc gcgcctcgac gaagcttcgg tcgaggtcgg
     5881 tatagaacaa cgacagccca tcggttggtt tggcgtctcc cgacgatgtg cgcgcgagga
     5941 tcagcatttt gtctgcaacc tgcgcggtcg atatccagat tttgcgcccg cgcacaatat
     6001 actgatcgcc cttgcgctgg gccgcggtgg taatgcttgc ggtattcagg ccggcgtccg
     6061 gttccgtcac cgcgaaacag gctttctgtt cgccgcggat catcgggggc aaccagcgac
     6121 ctttctgttc cgtcgtgccg aatacgacca ccggattaag gccgaagata ttcatatgca
     6181 cggctgatgc gccggaaaaa ccggcacccg attccgcgat cgcctgcatc atcaacgccg
     6241 cctcggtgat tccgaggccg gcgccgccat attcagatgg catcgcaatg ccaagccatc
     6301 ctccttgcgc cactgcccga taaaagtctt ccgggaagcc gccgtcgcga tcgcgctcga
     6361 gccagtattg cgcatcgaac tttgcacaga ctttggcaat cccctcggta atcgcgagct
     6421 gttccggtgt cggagtaaag tccataagtc gcaaaaagcc ctggcggcta catcacgcta
     6481 ctttggttac gcccttgcgg accaattcgg ctatctcgac ttcgctgtag ccggcttccc
     6541 gcaacacctc ggcggaatgc tcgcccagac acggggcagg acgcgggact gcaggctgcg
     6601 actcggacca attggtcggt gtgcgcatcg cgcgcagttc accttcgctc ggatgatcct
     6661 cgatggtgaa aaagccgctt ttcaggagat gatggtcctg cacaacatct tcgatcgagt
     6721 tcatgcgcgc gaccggaatg tcgacgcttt ccagcaatgc catccattct gcagtagtgc
     6781 gcgttctgaa cagttgcgtt aaaaacgcat agatatcgcg gatgtttgcc gcgcggctgg
     6841 cctgcgtagc gaagcgcgaa tcctgcagca actccggccg gccgatcacg cttgcgaaac
     6901 ttttccactg tttgtcgttg tagaccagca cgcacaagta gccatcgctc gtggggtacg
     6961 gtttgcgcgc cagcagccgc tgatagccgc ccggcccttc cgccggatcc caggtaaatc
     7021 cggcaaggtg gtcgcccagc acaaaatgcg caaccgactc gaacatcggc acttcgatgg
     7081 attcgccacg gccggtgcgg ctgcgctgga agagtgcagc ggtgactgcg tatacggcat
     7141 gcaggccggt gagccggtcc gcgagattga ctggcgcata gtttggcgcg gcgccagtct
     7201 gggccaccag ccaaggcacg ccggaaatgc cctggatcaa atcgtcatag gctgctttgg
     7261 cagcgtaggg accgttctgt gaatagccgt acgctccaac gtagagaatc tcaggattga
     7321 gcgcgctcaa ttcttcgtac gagaggccca gccgcgccat
//
LOCUS       DATMZQ010000472        10827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4747010, whole genome shotgun sequence.
ACCESSION   DATMZQ010000472 DATMZQ010000000
VERSION     DATMZQ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4747010"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VN496_15025"
     CDS             <1..474
                     /locus_tag="VN496_15025"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HXF18318.1"
                     /translation="QMRRSGLVLLLATVALFQGCAGSPTVSEQATVRADNRVASSATG
                     YAKEMLGKPYRYAGDTPAGFDCSGLVKYSYGRAGIAMPRDTQSQREISVLVSVRNLRA
                     GDLLFFDQEGKKTSHVGIYIGNGRFIHAPSSGGKVRTDSLNSEFWKKHFVEARRI"
     gene            501..1601
                     /locus_tag="VN496_15030"
     CDS             501..1601
                     /locus_tag="VN496_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18319.1"
                     /translation="MTRKSSTGVAVLMATLSLHAGAAGPDHVDITWMSIANIHYQIGP
                     VGILTDGYITRIPEKEFHGGGGGYAYTRHPWKPDVPAVARVLDAMGGPDSVKLLLTGH
                     SHWDHSFDTATWSRLTGAPIIGSQTTCFEVMAENLPAERCRAVYGLEKIEIAPGVTMR
                     VVRWNHSGDPAKNWEQHDPVELEAIPRQDPATGGLKAGVAEDFPNGGGNRGFLFTVDG
                     PQGRYSWFYHNSASAVDIDVPIIIKGVDYGAPIENLKRAMRDAGLDSVDLWIGTGGLP
                     VAQLVVPVLKPKAHIPIHWDGLWESFEGGMPWAFSDPPDEDFLAKSGVKLIKPGQYMD
                     KWRLDATGLRAVANSQVKRALGFADVQQLRAR"
     gene            complement(1645..2055)
                     /gene="mscL"
                     /locus_tag="VN496_15035"
     CDS             complement(1645..2055)
                     /gene="mscL"
                     /locus_tag="VN496_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXF18320.1"
                     /translation="MSIASEFKEFALKGNVMDLAVGIIIGAAFGKIVDSVVNDLIMPL
                     IGKLTGGLDFSNYYIGLNGQPSGLPLADAKKAGAVFAYGNFITVLLYFIILAFIIFVM
                     VKQMNRLKRQAPATPETTPEDVLLLRQIRDSLQK"
     gene            2374..3876
                     /locus_tag="VN496_15040"
     CDS             2374..3876
                     /locus_tag="VN496_15040"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXF18321.1"
                     /translation="MFSKLKARKWLIAALVPAVFGVYYEFALPGHTTVQAAQPGSARA
                     STSSTQARLPDFTRIVEENGPSVVNVSVSRSQPSLAQGQRPGVPGLAPDDPLQEFFRR
                     FGQPGQPGQPGEQGQPAEPEIQRGLGSGFIISSDGLILTNAHVVADATEITVKLTDRR
                     EFEGKVLGVDKRTDIAVLKIQAKNLPAVRLGKASDLKVGEWVAAIGSPFGFDNTITSG
                     IVSAKARSLPDENYVPFIQTDVAVNPGNSGGPLFNMDGEVVGVNSQIFSRTGGFMGLS
                     FAIPIDVALQVKDDLVQNGKVTRGRIGVQIQDVNQALANSFGLPKPQGALISGVEPGS
                     PADKAGLKSGDVVLGVNGKQVNQLSELSGAIAATKPGSNARLQIWREGKSRDVDVKVA
                     ELLEQKTAAANGAKNAPGETSAKLGLTLRELAPEERAQLKTEGGLVVQSATGAAARAG
                     IQPGDVILALNDTPVKSVEQLKSLVQKSDKTVALLIQRETNRMYVPLQIG"
     gene            4193..5716
                     /locus_tag="VN496_15045"
     CDS             4193..5716
                     /locus_tag="VN496_15045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXF18322.1"
                     /translation="MPDNTREQARDNSDLSPELIEFVRALQAALIDKRVRQQLQPIDN
                     ERPSKLESAFSAQQAKQAIRPELASHPQLLAAVVDSTDVGIVSETTNGVLLSWNKGAE
                     HTFGYSAAEMVGNSRACLIPEFLKAEDAKLTRQVIDGKAALNRETVWQHKDGKFVDVS
                     VSFAPVVEHDVVTRLSMVVSDITERKRMERALMESERKQRSRAAELKIVLDAVPAAVC
                     IANDAECKSITGNRMAYDLLRLPQGSNLASELADNDKSPSFRIFRNGRVLEAHELPIQ
                     MATFRGIEVRDFEQDVVFDDGTIRHLIGNAAPLRDAAGKVYGAVGAFIDITTRRNMEE
                     QIRRMAHFDSLTNLPNRILLMDRLEQALAISQRNHSKTSVIFMDLDHFKKINDNLGHH
                     VGDMLLQQVAERIRGALREVDTVSRLGGDEFVMVLPELRHTEDATTIADKLLRALDED
                     FFITGEKIRISPSLGISIYPDNASDAGTLIRLADKAMYHAKQAGRNTYRFYDEAMKS"
     gene            complement(5742..7442)
                     /locus_tag="VN496_15050"
     CDS             complement(5742..7442)
                     /locus_tag="VN496_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXF18323.1"
                     /translation="MADDNAKRGIRRNLTNYGDPDFAAYLRRSFARSMGYSKDLLGRP
                     IVGIAQSASGFNNCHRYVPELVEAVKRGVLKSGGLPIDFPTISLGEVFLSPTSLMFRN
                     LMAIDVEEMVRAQPMDAVVLIGGCDKTVPAQLMGAISADVPAIQLVTGPMMAMPFRGE
                     RLGACTDCRRFWGKYRAGEVSDASIDEIEGNLATTAGTCAVMGTASTMACVAEALGMS
                     LPDTAAIPAVHADRIRAAEATGVCAMRMIEAKGPRPSEIVTERSVENALRVLLAIGGS
                     TNAIIHLTAIAGRRGVKVSLERLNRMSDETPVLIDLKPTGSHYMQDFHAAGGMSALLA
                     ELKDLLHLDCQTVSGETLGDRLARPVAFIDRGVIRARSEPISPVGGLVALFGSLAPGG
                     AILKRAAADEGLFEKEGRAVVFESLEDLSARIDTPELDVTKDDFLILKNAGPRSASGM
                     PEAGYLPIPRKLAQKGLKDMVRISDARMSGTAFGTVILHITPDAASGGPLALVQSGDR
                     IRLSVKHRKLDLLVSDAEIANRRSKLTASPLPARGYARLYQQHILQADAGCDFDFLRA
                     "
     gene            7509..8201
                     /locus_tag="VN496_15055"
     CDS             7509..8201
                     /locus_tag="VN496_15055"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXF18324.1"
                     /translation="MRTLAIILLLGLLGPAYAESPHPDFSHSRISLDARASEEIANDV
                     MRATLFIEMEDADAGKLAQKINRATNDALKLAKTFTGLHAKTAGYSTYPVTEKNKIVR
                     WRSRSEISMQSEDFPRMTDAISQLQALMQLGAVSFSVSPAARASVEETLTQAAITQFL
                     HKAETATRGFGGSNFVVTEATIATEGANLPSPRPMAVLRSASAAQDNAPAFEAGSSQI
                     TVIVNGSIVIPR"
     gene            complement(8209..8976)
                     /locus_tag="VN496_15060"
     CDS             complement(8209..8976)
                     /locus_tag="VN496_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18325.1"
                     /translation="MAKGLRIAVLLFVLATVAVSAWQKRTLTTEWKRTLDVVVYPING
                     DGRETTSAYIARLTASSFYPIVEFARREAQRYEVGLASPVSVKLGPLVNSIPPDTPPG
                     HATPGIMLWSLRLRFWAWRNDSFAGPKPDVRVFAVYFDPAFTQRLEHSVGLKEGLIGI
                     ARVFAASHMTEENNVIIAHEILHTFGATDKYDPATTQPVYPDGYAAPGQSPLYPQQFA
                     EIMGGRIPMSQAQSETPQALDFAIVGEKTAAEIHWRH"
     gene            complement(8987..10159)
                     /locus_tag="VN496_15065"
     CDS             complement(8987..10159)
                     /locus_tag="VN496_15065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXF18326.1"
                     /translation="MSEAVIIEAVRTPFGRRGGALREYRPDALLAHTLDSLIERARVD
                     PRKVEDVVDGTVTLAGEQGANPARLAVLLSKLPVEVPGVSLNRMCGSSQQAVHFAAQT
                     IAAGDMDYVIGCGVESMSRVPMFLDIGLGGPVSGFEALNPELFNRHELIHQGESAERV
                     ADKWKLSRQAVDEFAKESHRRAALAQKSGLNHELIPVNGVSPEGKPIAMDRDEGVRAQ
                     IDEAKMNSLKPAFRPEGKGVVTAANSSQISDGASAVLVASARAARADGLRPKARFRSR
                     VAVGSDPTLQLTGVIPATRLALSKAGLTIQDLDWIEINEAFATVVLSWAQELKPDMAK
                     VNPWGGAIAHGHPLGATGAGLMAKLLAGLEATGGTLGLQVMCIGHGQATATVIERI"
     gene            complement(10387..>10827)
                     /locus_tag="VN496_15070"
     CDS             complement(10387..>10827)
                     /locus_tag="VN496_15070"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HXF18327.1"
                     /translation="GSLAEAVAATSFAIEGVAGDIARKVTAGFEAYRGQPGVDMGPKT
                     YKWFREHAHYDDDHPKIAMEIVKRYATTERMQTKVMLAAKRSIQLLHNALLTGYQAYS
                     PSESSEDTAETELRVMDRRAQHIRLAFPDRRFGERRGFKLRKVA"
BASE COUNT         2340 a         3178 c         3088 g         2221 t
ORIGIN      
        1 cagatgaggc gcagcgggct ggttttgctg ctggccacgg tcgcattgtt tcagggttgc
       61 gccggttcac ccaccgtatc ggagcaggca accgtacgag cggacaaccg ggtcgcatca
      121 agcgccaccg gatatgcgaa ggaaatgctc ggcaaaccgt atcgatacgc cggggacacc
      181 ccggcaggtt ttgattgcag tgggctggtc aaatacagtt atggccgagc cggaattgcg
      241 atgccgcggg atacgcagtc tcagcgggaa atatcagtgc tggtgtccgt gcgcaatttg
      301 cgtgcaggcg atctgttgtt cttcgaccag gaaggcaaaa agacatcgca tgtcggcatc
      361 tatatcggca atggccgctt cattcatgcg ccgtctagcg gcggcaaagt ccgcaccgac
      421 agcctgaatt cggagttctg gaaaaaacac ttcgtcgaag cgcgccggat ctgacaagaa
      481 tttcgctgaa gggattttcg atgactcgca aatcttctac gggtgttgcc gttctgatgg
      541 cgaccctgtc tttgcacgcc ggcgcggctg ggccggatca tgtcgacatt acgtggatgt
      601 cgatcgccaa tatccattac cagataggac cggtcggaat actcacagac ggctacatca
      661 cccgcatacc ggaaaaagaa tttcacggag gcggcggtgg atacgcttac acacgtcatc
      721 cctggaagcc cgatgtccct gctgttgcac gcgtgctgga tgcaatgggc ggccccgaca
      781 gcgtcaagct gctgctgacg ggccatagcc actgggatca ttcctttgat acggcgacct
      841 ggtccaggct gacgggcgcg ccgatcatcg gctcgcagac cacctgcttt gaagtcatgg
      901 cagagaatct gcctgctgaa cgatgccgtg cagtgtacgg actggaaaag attgagatcg
      961 cgccaggtgt caccatgcgc gtggtgcgct ggaaccacag cggtgatccc gcgaagaact
     1021 gggaacaaca tgacccagtc gaattggagg ccatcccccg ccaggatcct gcaactggag
     1081 gcctgaaagc tggcgtggcg gaggactttc ccaatggcgg cggcaatcgc ggatttttgt
     1141 ttactgtgga cggtccgcag ggccgctaca gttggttcta ccataattcg gcaagcgcag
     1201 tggacatcga cgtgccgatc atcatcaaag gagtcgacta cggggcaccg attgaaaatt
     1261 tgaagcgggc gatgcgcgat gccgggctgg attcggtcga tctctggatt ggaaccggtg
     1321 gtctgccggt tgcacaactc gtggtgccgg tcctcaagcc caaagcccat attccaattc
     1381 actgggacgg gttgtgggaa tcattcgaag gcggcatgcc gtgggcgttt tccgatccgc
     1441 cggatgagga cttcctggca aaatccggcg tgaaattgat caagcccgga cagtacatgg
     1501 acaagtggcg cctcgatgca accggactcc gggccgtcgc aaacagccag gtcaagcgcg
     1561 cgcttggctt tgcagacgtg cagcaacttc gcgcacgtta aaacgcgcaa cttcttgtac
     1621 agtttagcgg caccgaaact ctttttattt ttgcaaactg tcgcgaattt gccgtagcag
     1681 caatacgtct tcgggcgtgg tttcaggtgt tgccggcgcc tgcctcttga gcctgttcat
     1741 ctgcttgacc atcacaaaaa taatgaaggc aagaataatg aagtacagca gcactgtgat
     1801 gaaattgccg taggcgaata ctgcaccagc tttttttgca tccgccaagg gcaagcccga
     1861 cggctggcca ttcaacccaa tatagtagtt gctgaagtcc agaccgccgg tgagttttcc
     1921 gataagcggc atgatcaggt cattcacgac ggaatccact atcttgccga acgcggcgcc
     1981 gataattatg ccgacagcaa gatccatgac attgcctttc aaggcaaatt ccttgaactc
     2041 gcttgctatg ctcattgctg ctcctcacag gatagaacgg gattgctcgg ccgcggcacg
     2101 atgcctggcg cgcaaatgct actcgaatag ttgagatcct cccaatgtct tatgtattcc
     2161 cgctccagcc agcgcaacga caacaaatcc gcggatgtgc ctgccgctgg tctgcccaac
     2221 tccttgtttc tggcggcgcc tttaccaacg ataagactcc ttgccgtcgg tgtagcactg
     2281 cgtgaactta ggcgctgctg caatggtctg aggtcgagta aacagttttc atcgcgggca
     2341 actgcccgct ttgcatttca ggagatctcc tcaatgtttt ccaaactcaa agcaagaaaa
     2401 tggctgattg ccgcactggt tccggcggtt ttcggcgtct attatgaatt tgcgctgccg
     2461 ggccacacca cagtccaggc cgctcaaccg ggcagcgctc gcgcgagcac ttcatcaacg
     2521 caggcgcgat tgcctgactt caccagaatt gtggaagaga atggtccttc cgttgtcaat
     2581 gtgagcgtat cccgcagcca accatccctg gcgcagggcc agcgacctgg cgttccaggg
     2641 cttgctccgg acgatccctt gcaggagttc ttccgccgct tcggacagcc ggggcagccg
     2701 ggacagccgg gtgagcaagg acagcccgca gagccggaaa tacagcgcgg cctcggctcg
     2761 gggttcatca tcagttccga cggcctcatt ctgaccaatg ctcacgtagt ggccgatgca
     2821 actgaaataa ccgtcaagct caccgatcgg cgagaattcg agggcaaggt actcggcgta
     2881 gacaagcgca cggacattgc ggttttgaag attcaggcca agaatttgcc cgcggtgagg
     2941 ctgggcaaag ccagcgacct gaaggtcggc gagtgggttg ccgccatcgg atcgccattc
     3001 ggttttgata acaccatcac ctcaggcatt gtcagcgcca aagcgcgttc gctgccggac
     3061 gaaaactatg ttccgttcat ccagacggac gtggcggtga acccgggcaa ctccggtggt
     3121 ccgctgttca atatggacgg cgaggtagtc ggcgtcaatt cgcaaatctt tagtcgtacc
     3181 ggcggtttca tgggattgtc gttcgcgata ccgatcgacg ttgcattgca ggtcaaggat
     3241 gacctggtgc aaaacggcaa ggtcacgcgc gggcgcatcg gcgtgcagat acaggacgtg
     3301 aatcaggcgc tcgccaactc cttcggactg cccaagccac aaggcgcatt gatcagcggc
     3361 gttgaaccag gcagcccggc ggacaaggcc ggcctgaaat cgggcgacgt agtcctgggc
     3421 gtcaatggca agcaagtaaa ccagctctcc gaactctcgg gggcaattgc ggcgaccaag
     3481 ccgggcagca atgctcgcct tcagatctgg cgagaaggca aaagccgaga cgtcgacgtg
     3541 aaggttgccg aactcctgga gcaaaaaact gctgccgcca atggcgctaa aaatgcccct
     3601 ggtgaaactt cggcaaaact tggtttgacc ttgcgtgaac tggcgccgga agagcgcgcg
     3661 caacttaaga ccgaaggcgg cttggtagtg caaagtgcca caggcgctgc ggcacgggcc
     3721 ggcatacagc ctggcgacgt catcctggcg ttaaacgaca cgccggtaaa atcggtcgag
     3781 caattgaaaa gtctggtcca aaaatcggac aagaccgttg ccctgttgat acagcgtgaa
     3841 actaatcgta tgtacgtacc cttgcagatc ggctgaacgt aagggttcca ttccagtttc
     3901 cagccccaaa gggcgtgctg cgagagcggc gcgccctttt ttttctgggc ttattggttt
     3961 attcgggcaa tgcctgccct gaaaacgggg aaaaaggcga tcgctaaacg ggggtgctca
     4021 aaaaataggc gggaaaaacc tgcaccaggc ggtgttatct ttgaacccca aggctggcgc
     4081 ggcgccttgc gacagcctat gattgatggc tcaattcctg cctataaaac ctataagttc
     4141 cactgtggca gtgccgatta accaacgcag ccggctttta aggggggccg acgtgccaga
     4201 caatacaagg gagcaggcaa gggacaattc cgacctcagt ccggaattga tcgagttcgt
     4261 gcgcgctctg caggctgcgc taatcgacaa gcgggtacgc cagcaactcc agcccataga
     4321 caatgagcgg ccttcgaaac tggagtccgc tttttccgcg cagcaggcca agcaagcaat
     4381 ccgtccggaa ctggcaagcc acccccagct gcttgccgcc gtagtcgatt ccaccgacgt
     4441 cggcatcgtc agcgaaacaa ccaacggcgt acttctctcg tggaacaaag gggccgaaca
     4501 cacgttcggc tacagcgctg ccgaaatggt aggcaacagc cgcgcctgcc tgatccccga
     4561 attccttaag gcggaggacg cgaagctcac gcgccaggtg atagacggca aggccgcact
     4621 gaaccgcgag acggtgtggc agcataaaga cggaaaattc gtggacgttt cggtgagctt
     4681 tgcgccggtg gtggagcacg acgtcgtaac ccggctttcc atggttgtca gcgatatcac
     4741 cgagcgtaag cgcatggagc gagcgctcat ggaaagcgag cgcaagcaac gtagccgcgc
     4801 agccgaactg aagattgtgc tggatgccgt tccggcagcg gtgtgcatcg caaacgatgc
     4861 tgaatgcaag tcgatcaccg gcaatcgcat ggcttacgac ctgctgcgcc tgccacaagg
     4921 cagcaatctt gccagcgagc tcgctgataa cgataaatcc ccctcattcc ggatctttcg
     4981 caatggccgc gtgctggaag cacacgaatt gccaatacag atggctacct tccgcggtat
     5041 cgaagtgcgg gacttcgagc aggacgttgt gtttgacgat ggcaccatcc ggcatttaat
     5101 cggcaatgcc gcgccgctac gggatgccgc cggaaaagtg tatggcgccg taggcgcttt
     5161 catcgacatt acgacgcgac ggaacatgga agagcagata cgccgcatgg cgcacttcga
     5221 ttccctgacc aatctgccga atcgtatttt gctgatggac cggctggagc aggcgctcgc
     5281 gatttcgcag cgcaaccatt cgaagacttc agtcattttc atggatcttg accacttcaa
     5341 aaaaatcaac gacaaccttg gccatcatgt gggcgacatg ctgctgcagc aggtggccga
     5401 gcgcatacgc ggcgctttgc gggaagtcga tacagtttcc cgtctgggag gcgatgaatt
     5461 tgtcatggtg cttccggaat tgcgccatac cgaagatgcc accacgattg cagacaagct
     5521 gttgcgggca ttggacgagg atttttttat tacgggcgaa aaaatacgca tctcgccgag
     5581 tctcggcatc agcatctatc ccgacaacgc gagtgatgcc ggcacgctga tccgacttgc
     5641 ggacaaagcg atgtatcacg ccaagcaggc cggcaggaat acctatcgct tctatgacga
     5701 agcaatgaag tcctgagctg gcgaatctgg ttacggatgc gctaagcgcg tagaaaatcg
     5761 aaatcacaac ccgcatcggc ctgcaggatg tgctgctgat acaagcgcgc gtaccctctc
     5821 gcgggcaatg gcgaggcggt cagcttgctg cgacggttgg ctatttcggc atcgctgacc
     5881 aataaatcga gttttctgtg cttaaccgac aggcgaatgc gatcgccgct ttgcaccaaa
     5941 gccaacggcc cgccggaagc agcgtccggc gtgatgtgca gaatgaccgt gccgaacgca
     6001 gtgccactca tgcgtgcatc ggatattctg accatgtcct tcaatccttt ttgcgcaagc
     6061 ttcctgggaa tgggcaaata gcccgcttcg ggcattccgg acgcgctgcg cgggcccgca
     6121 ttcttcaata tcagaaaatc gtccttggtg acgtcgagct ctggcgtatc gatgcgtgca
     6181 gacagatctt ccagggactc gaacactact gctctgcctt ctttctcaaa caagccctca
     6241 tcggctgcag cccgcttgag aatggcgccg ccgggcgcaa gacttccgaa cagcgcgact
     6301 aacccgccga cgggactgat cggctcagag cgcgcgcgaa tcaccccacg gtcgatgaaa
     6361 gctaccggcc gcgcaagccg gtcgccgagt gtttcgccgc ttacggtctg gcaatccagg
     6421 tgcagcaaat ctttcagttc ggcaagcagc gcactcattc cgccggcagc atggaaatcc
     6481 tgcatgtagt gactgccggt aggcttgaga tcgatcaaca ccggcgtttc atcggacatg
     6541 cggttaagtc gctcgagcga caccttgacg ccgcggcgcc cggcaattgc cgtcaaatgg
     6601 ataatcgcgt tggtcgatcc cccgatggca agcagtacac gcaacgcatt ctctaccgat
     6661 cgttcggtca cgatctcgga cggccgcgga cccttggctt ctatcatgcg catggcgcaa
     6721 acgccggtgg cttccgcggc gcgaattcgg tcggcatgca cggcgggaat cgccgcggta
     6781 tccggcaggc tcattccgag cgcctctgcc acgcatgcca ttgtgctggc cgttcccatg
     6841 actgcacagg tgcctgctgt agtcgcaaga ttaccttcga tttcgtcaat gcttgcatcg
     6901 ctgacttcac cggcccgata tttgccccaa aaacgccggc aatctgtgca cgcgcccagc
     6961 cgctcaccgc ggaaaggcat cgccatcatt ggaccggtga ccagctgaat cgccggcaca
     7021 tcggctgaaa ttgcgcccat caactgcgcc gggacggttt tgtcgcagcc acctatcagc
     7081 acgaccgcat ccattggttg cgcgcggacc atctcctcga cgtcgatcgc catcagatta
     7141 cgaaacatca ggctggtcgg actcaaaaaa acttccccga gcgagatcgt cggaaagtcg
     7201 attggcagtc cacctgattt aagtaccccg cgcttcaccg cctctaccag ttcgggtaca
     7261 tagcgatggc agttgttgaa gccgctggcg gattgcgcga tgccgacgat gggccggccg
     7321 agcaagtctt ttgagtaacc catggaccgg gcgaaagagc gtctgagata ggcggcaaaa
     7381 tcgggatcgc cgtaattggt aaggttgcgg cgaatgccgc gctttgcatt gtcgtcggcc
     7441 atgatgttgt gtcggcaaat gagccgctat cataggttcg ccgatcgttc acgccaaccg
     7501 gagattttat gagaacgcta gcaatcatcc tgttgctggg cctgcttggc ccggcttacg
     7561 cggaatcgcc tcacccggat ttcagccatt cacgcatcag cctggacgcg cgggccagcg
     7621 aagaaatcgc caacgatgtg atgcgcgcca cgctgtttat cgagatggag gatgcggatg
     7681 ccggcaaact ggcacaaaaa atcaatcgtg cgaccaacga tgcattgaaa ttagccaaga
     7741 cattcaccgg actgcatgca aaaacggccg gctattcgac ttacccggtc accgagaaaa
     7801 acaagatcgt gcgttggcgt tctcgctcgg agatatcgat gcaaagcgag gatttcccgc
     7861 gcatgacgga tgcaatcagc cagctgcaag ctttgatgca attgggcgcc gtcagtttct
     7921 ccgtttcacc ggctgcccgc gccagcgtcg aggaaacgct cacgcaagcg gccattacgc
     7981 agtttctgca caaggcagaa accgccacca ggggtttcgg gggatcgaac ttcgttgtga
     8041 ccgaggcgac cattgcaaca gaaggcgcca acttgccttc acctcgaccg atggccgtgt
     8101 tgaggtccgc gtcggcagca caggacaacg cgcccgcctt tgaagccggc agcagtcaaa
     8161 tcaccgtgat cgtcaatggt tcgatcgtga ttccgcgctg aagtccggtc agtgtcgcca
     8221 atgaatttca gcggccgtct tttctccgac aatcgcgaaa tccagcgcct gcggcgtttc
     8281 tgactgcgct tgcgacattg ggatgcgacc gcccatgatt tcggcgaatt gctgtggata
     8341 gaggggagat tggccgggtg cagcataccc atccggatac accggttgcg tggttgccgg
     8401 atcgtacttg tccgtggccc cgaaggtatg cagaatctca tgcgcaatga tgacgttatt
     8461 ctcttcggtc atgtgcgatg cggcgaacac gcgtgcgatg ccgatcaaac cttccttgag
     8521 accaacggaa tgctccagcc gctgcgtgaa ggcgggatcg aagtacacgg cgaacacgcg
     8581 cacatcgggt ttcggacccg caaagctatc gttgcgccat gcccagaacc gcagtcgcag
     8641 gctccacagc atgattcctg gcgtcgcatg gcccggcggt gtgtcgggcg gtattgaatt
     8701 gacaagcggt cccagtttta cgctcacagg gctcgcaagc ccgacctcat agcgttgcgc
     8761 ctcgcggcgt gcaaattcga cgatcggata aaacgaactg gcggtgagcc tcgcgatata
     8821 cgcagaagtt gtttcgcgtc cgtcgccatt gatgggatag accactacat ccagtgtccg
     8881 cttccactcc gtggtcaggg tccgcttttg ccaggcacta accgccaccg ttgcgagcac
     8941 gaacagcaac actgcgatcc gcagcccctt tgccatcgcg aagtgttcag atgcgttcta
     9001 tgaccgtagc cgttgcctgc ccgtggccga tgcacatgac ctgaaggccc agcgtgccac
     9061 cagtcgcctc gagaccggcc agcagtttgg ccatcaagcc tgcaccagtc gcgcccaatg
     9121 gatgcccgtg cgcgattgcg ccgccccacg gatttacttt ggccatgtcg ggcttcaatt
     9181 cctgtgccca ggaaagcacc acggtcgcga acgcctcatt gatttcaatc cagtcgaggt
     9241 cctgaatcgt cagcccggct ttgctcaacg ccaggcgcgt ggccggaatg acgccggtga
     9301 gttgcagcgt cggatctgaa ccaaccgcca cccgcgagcg aaaacgcgct tttggccgca
     9361 agccatccgc tcgcgccgcg cgtgcgctcg cgacgagcac ggcggatgcg ccatccgata
     9421 tctggctcga attggcagcc gtaaccacgc ctttgccttc aggacggaag gcaggcttca
     9481 gcgagttcat ttttgcttcg tcaatctgcg cgcgcacgcc ttcgtcccgg tccatggcaa
     9541 ttggcttgcc ttccggcgat accccattta ccggaatcag ttcatggttc agtccggatt
     9601 tttgcgcgag cgcggcgcga cgatggcttt ccttggcaaa ttcgtcgacc gcctgccgcg
     9661 acaatttcca tttgtcggcc acacgttcgg cgctttctcc ctgatggatc agttcgtggc
     9721 ggttgaacag ttcgggattg agcgcctcaa aaccgctgac cggaccaccc aatccgatgt
     9781 ccagaaacat cggcacgcgg ctcatgcttt ccacgccgca cccgatgacg taatccatgt
     9841 cacccgcggc gatcgtctgt gcagcgaaat gcaccgcctg ctggctcgaa ccgcacatgc
     9901 gattcagcga cacgccgggc acctccaccg gcaatttcga cagcaatacg gccagccgcg
     9961 ccgggtttgc accttgttcc ccggccagtg tcacggtgcc gtccaccacg tcctcgacct
    10021 tacgtggatc gacgcgcgcg cgctcgatca ggctgtccag cgtgtgcgcc agcaatgcgt
    10081 ccggccggta ttcccgcaaa gcacccccgc gccgcccaaa cggggtgcgc acggcttcaa
    10141 taatcactgc ctcgctcata aaaactcccg tgccaatgtg tgaaagctgg ataccattcc
    10201 tcgtcacgag cagactatat cttgaaccga taggccatgc gcaacgcacg ttttgacatc
    10261 cgcggggttt attagagtgg gaaagcagca atcgacgtca atcctattcc agcggttcag
    10321 tggaaaataa ggccagcata ttagctggcc ttattcagac tagtcgagat aacgcgattg
    10381 cccgcactac gcgacttttc taagcttgaa tccgcgccgc tcgccgaaac gtctgtccgg
    10441 aaacgccagc cgtatgtgct gcgctcgccg gtccatcaca cgcaattccg tttcggcggt
    10501 atcttccgaa ctctccgatg gagagtaggc ctgataaccg gtcaatagcg cattgtgcag
    10561 gagctgaatg ctacgcttgg ctgcgagcat caccttggtc tgcatgcgct ctgtggtcgc
    10621 gtaacgtttc acgatttcca ttgcgatttt ggggtgatcg tcatcgtaat gcgcgtgctc
    10681 gcggaaccat ttgtaggtct tgggccccat atcaacgccg ggttgacctc gataggcttc
    10741 gaagcctgcc gtaactttgc gggcgatatc tcccgccacg ccttcgatcg cgaagctggt
    10801 tgccgccacc gcctccgcaa gcgagcc
//
LOCUS       DATMZQ010000473         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_949464, whole genome shotgun sequence.
ACCESSION   DATMZQ010000473 DATMZQ010000000
VERSION     DATMZQ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949464"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VN496_15075"
     CDS             <1..418
                     /locus_tag="VN496_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXF18328.1"
                     /translation="VQGSGTALRMTAANFISEFRNSLPLQEELYRFTFAKLSQARQTA
                     ACNCFHAVDARLARWLLMTSDRMRSDQFLLTQAFLADMLGVRRAAVSAASSDLQRRHL
                     ISYSRGTIGIIDRKGVKALSCECYETMKSMHRSIPL"
     gene            542..751
                     /locus_tag="VN496_15080"
     CDS             542..751
                     /locus_tag="VN496_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18329.1"
                     /translation="MARYVAKAQVASKTAGFGFDEWEVAKRYAHSLPKGTLVQITDTE
                     AQGSQALTYLITAGIEEAQLLRQAT"
     gene            1134..2297
                     /locus_tag="VN496_15085"
     CDS             1134..2297
                     /locus_tag="VN496_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXF18330.1"
                     /translation="MLYQFLSEHETDLSERCRVKAAKRPMPAETKEALFNGVPVFIRQ
                     LIYTLETEEKAGRPVDRRLSSGLADGGRRKASEMSKSATIHGRELLDHGFTVDQVVHA
                     YGDVCQSVTDLACELAVPFEINEFRTLNRCLDDAIADAVTEFSYQREVLMTGKQNDSF
                     NMRLGSFAHELRNLIQTATLSFAAIKAGTVGLSGSTGAMMDRTLVAMRDMVDGSVAEV
                     RMDAGMAMHSQRFAVADLILEVKISASLEAELHHCLFSVSSVDPELAIEADRHLLSAA
                     IGNLLQNAFKFTHPGTEVTLTAYGAGDRILIDVADHCGGLPWSDAEKVFAPFSQGGKD
                     KRGLGLGLSIARRSVEANHGSLRVHDRPGQGCVFTIDLPRHAMQEPSRDARKQ"
BASE COUNT          602 a          712 c          695 g          587 t
ORIGIN      
        1 cgtgcaggga tccggcaccg ccctgcgaat gaccgcggcc aatttcatta gtgaattccg
       61 caacagcctt ccattacagg aagaactcta ccgctttacc tttgccaagt tgtcacaggc
      121 aaggcaaaca gccgcgtgca actgtttcca cgcggtggat gccagactgg cgcgctggct
      181 actaatgacc tcggaccgga tgcgctcaga ccagtttctc ctcacccagg cctttctcgc
      241 agacatgctc ggtgttcgtc gtgcagcggt gtctgccgct tcgagcgact tgcagcgacg
      301 acatctgatc agttacagcc gcggaactat cggaattatc gaccgcaaag gcgtcaaggc
      361 tctttcgtgc gaatgctacg agaccatgaa atccatgcac cgctccattc cgctgtagtt
      421 agttccctgg ttatcaggcc gacgtgcatc cttttgtgtg taaaaaagtt ttgactgttt
      481 acggttttgc gctcatgccg gcgtgggcca cttgccccgc attccacgac gatgaggcgt
      541 tatggcgcga tacgttgcga aagcacaagt agccagcaaa acagcaggat ttggattcga
      601 tgagtgggaa gtcgcgaaac gctacgccca ttcactgccg aaaggtactc tagtgcagat
      661 tacagatacc gaggcgcaag gtagccaagc gttgacatat ctcattacgg cagggatcga
      721 ggaagcgcaa ctactgcggc aagcgacgta acttgctcca agctctgtag ccttcaatgc
      781 aaggccccgt cgagcagtcc aggctctaga tccgacagcc gcatttttat ccaggtccga
      841 agctctggta tatcaatacc gtctcaccac cacacgaatg agttgcagta gctctgcgtc
      901 ggcaggcaaa acaaaatagc aataactgtc cctcgaacac ttacgatcga cccgccaatt
      961 cgcgggctcg aggccgcccg gcatatatac caactcgtac atatgcctca aaaaacatat
     1021 gcctgaaaca agcgacacct atagtttcgc tataacttgc cattaagcac ggtagcgcac
     1081 atacgcgtct gcaagtacag cagactataa ggtcttcgtg aagggagatg tcaatgcttt
     1141 accaattctt aagcgagcac gaaactgatt taagcgagcg atgccgcgtc aaggcggcaa
     1201 agcggcctat gcccgcggaa accaaagaag ctcttttcaa cggcgttccg gttttcattc
     1261 gacaactcat ctacacgctc gaaacggagg aaaaggccgg acgtcctgtc gatcgccggc
     1321 tttcttccgg cctggcggat ggtggaaggc gtaaggcgtc ggaaatgagc aagagtgcca
     1381 cgattcatgg ccgcgaactg ctggatcacg gcttcactgt tgaccaggtg gtgcatgcct
     1441 acggagatgt ctgccagtcc gtaaccgatc tggcgtgcga gttggctgtg ccattcgaga
     1501 tcaatgagtt tcgtacgctc aatcgctgtc tggatgacgc tatcgctgat gctgtcacgg
     1561 aattctcgta tcagcgtgaa gtgcttatga caggcaagca aaacgactcg ttcaatatgc
     1621 gattgggttc attcgctcat gaactgcgta atctcatcca gaccgccacg ctgagctttg
     1681 cggcaatcaa agccggcacg gttgggttgt caggatctac gggcgcaatg atggatcgca
     1741 cattggttgc aatgcgcgac atggtcgatg gctcggttgc cgaagttcgc atggacgctg
     1801 gcatggccat gcatagccag aggttcgctg tggcggactt gatccttgaa gtcaaaatat
     1861 cggcgagtct ggaagcggaa ctccaccact gcttgttctc tgtcagctcg gtcgacccgg
     1921 agctggccat tgaagccgac cgccatctgc tctcggcagc gatcgggaat cttttgcaaa
     1981 acgcattcaa gtttacgcac ccggggaccg aagtcaccct gactgcctac ggagccggtg
     2041 atcgcattct gatcgatgtc gcagaccatt gcggtgggtt gccctggagc gatgcggaaa
     2101 aggtgtttgc gcctttttcc cagggcggta aagacaagcg aggcttgggc ctgggactgt
     2161 caattgcccg gcgcagtgtt gaggcaaatc atggcagtct gcgcgtgcac gaccgtcccg
     2221 ggcagggctg tgtttttacc atcgatttgc cacgacacgc gatgcaggaa ccttcaaggg
     2281 atgcgcgtaa gcaatgattc ttgcggcata ttggaatgtt ggcttagaca tttcctcatg
     2341 cgcagtggaa actattggcg ctgagcaacc gggtcaaaac ggcggccagg cattcgtgtt
     2401 ccccgcatca ttccatgtca gtgtaaaagc ccaatcctcg tcactgaaaa cgtcttgctt
     2461 gctgtactcc atcggagcca cgtccgataa agccccaact cgcttgaaac cttgtgctct
     2521 cagatcgtct tccaacagct tcatcttggc aacggaaccg gcagtgactc gaatcatagt
     2581 tggttaccca attagc
//
LOCUS       DATMZQ010000474        10938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_950391, whole genome shotgun sequence.
ACCESSION   DATMZQ010000474 DATMZQ010000000
VERSION     DATMZQ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950391"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VN496_15090"
     CDS             complement(<1..732)
                     /locus_tag="VN496_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXF18331.1"
                     /translation="MFEKILIANRGEIALRVQRACRELGIKTVAVHSVADAEAKYVRL
                     ADESVCIGPAASADSYLNIPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFVF
                     IGPRPDTIRLMGDKVSAKNTMIKAGVPVVPGSEGALPEEPQEIVKIARKIAYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVAMTRQEAKSAFGNPTVYMEKFLETPRHVEIQVLADE
                     HRNAVYLGDRDCSMQRRHQKIIEEAP"
     gene            complement(790..1245)
                     /gene="accB"
                     /locus_tag="VN496_15095"
     CDS             complement(790..1245)
                     /gene="accB"
                     /locus_tag="VN496_15095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXF18332.1"
                     /translation="MDLRKLKKLIDLVQESGIAELEITEGEEKVRINRIGVQGHPAAV
                     ALPQPPILAAQSPTAAPLAAPSVPEATEPEGHVVKSPMVGTFYRSSAPGSKPFVEIGQ
                     TINAGETLCIIEAMKLLNEIEADQTGVVKKVLVENGQPVEYGQPLFLIG"
     gene            complement(1254..1748)
                     /gene="aroQ"
                     /locus_tag="VN496_15100"
     CDS             complement(1254..1748)
                     /gene="aroQ"
                     /locus_tag="VN496_15100"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HXF18333.1"
                     /translation="MTTTLKGGKVRPSPASKSRKVLVLHGPNLNLLGTREPQIYGGEK
                     LKDLDDRLVSQAAGAGIVLETYQSNSESELIDRVHNARLTGVDFIVINPAAFTHTSVA
                     LRDALAAVRIPFIEVHLSNVHAREPFRHKSYFSDLAVGTICGLGSKGYEYALAHAMAY
                     TPSK"
     gene            complement(1865..4144)
                     /gene="dsbD"
                     /locus_tag="VN496_15105"
     CDS             complement(1865..4144)
                     /gene="dsbD"
                     /locus_tag="VN496_15105"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HXF18334.1"
                     /translation="MNHPGARFFLLLTLLASPQFTPGWAADGLLAPDTAFRVSASRLD
                     EKSIQVRYQIADGYYLYRDRFHFAIAEGPVKLGTPDLPAGTIKDDEFFGRVPIYRGDV
                     AIDLPLKSSLPAAGLTLKVVSQGCADIGVCYMPLTQTVRIEPATPASTIDNGLPSPRK
                     PAGLLARLQNGTGPAADEQEFLPVEKAFNVEVRFADAQTLMARLSPADGYYLYRDKIQ
                     VKTVPGTIATIDTVQIPRGETKQDPNFGKTEIFRVPVQVIIRLREAIQEQTTLAVNVG
                     FQGCSEKGLCYPPAIRQLPVTYVPFSTADPGGLSANGSTSLQSPGTFTREPAEDAKIA
                     RFMKTGNFWLIMGSFLGFGLLLSLTPCVLPMVPILSGLIAGQGKKITRMRGATLSAIY
                     VLGMALSYAIAGVIAGLSGTLLSAALQTPWALISFAAIFVLLALSMFGLYELQLPVAW
                     QTGAADTSNRLTAGTLGGVFGMGAISAVIVGPCVAAPLAGALLYVSQSHDVVLGGLAL
                     FTMGIGMGIPLLVLGASAGTFLPKAGAWMQTVKNFFGVLLLAGAIWIIAPLIPVTVQM
                     LCWSVLLILSGIYLHAIDPLPPGASGSRRLWKGIGVIALLLGIVLLVGGLSGGRDILQ
                     PLSGLQTRTGQKDLEGPAFIPVASIAELEAAVIRAGKPIMVDFYADWCVACKEMERLT
                     FRDANVKRRLDNMLLLRADVTDGTAQNEALLKRFGLYGPPGVIFFDRHGKELEGLRVI
                     GYQPAERFLAVLADLEHFK"
     gene            complement(4171..4509)
                     /gene="cutA"
                     /locus_tag="VN496_15110"
     CDS             complement(4171..4509)
                     /gene="cutA"
                     /locus_tag="VN496_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXF18335.1"
                     /translation="MGVDTRSDLIIVLTNLPDRTSATKLAALLIEHRHAACVNILAEC
                     TSVYRWNGEVNMATEVPLLIKTTLSEYARLEEKIRDNHPYEVPEILGIPVAKGLPAYL
                     DWVNTESAVR"
     gene            complement(4549..5433)
                     /locus_tag="VN496_15115"
     CDS             complement(4549..5433)
                     /locus_tag="VN496_15115"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18336.1"
                     /translation="MRRLLIIGCGDVGLRLARALKGRWRIYALTHTEARFSELRTTGI
                     TPVSGDLDHPDTLHRIAALAQDILHLAPPPASGDRDTRTTHLIRALAKGGSLPQRLIY
                     ISTSGVYGDCAGEVVYETRPAQPSTTRARRRLDAEKQIRIWGTETGVQVSILRVPGIY
                     CAGRLPLARLQSGMPSLSSERDPYTNHIHADDLARILLAALVRGRGGRAYNASDDCWL
                     KMGDYFDIVAEQFNLPRPPRVSWEIAQNELPEQLLSFMRESRRLANDRLKKELRVRLR
                     YPSVQHGVVAAWDAMKTL"
     gene            5484..6518
                     /locus_tag="VN496_15120"
     CDS             5484..6518
                     /locus_tag="VN496_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HXF18337.1"
                     /translation="MSHQVTIKPSDHTFICTDEESVLAGALRAGFTLPYGCRNGACGA
                     CKGKILQGSVDYGNPQPYVLAEFEKKAGLALFCQARPLTDLVIEAREIRGVGQLDIRK
                     LPCRVQTIHRVAPDVVVLKLKLPANERLQFLAGQYIDILMKDGKRRSYSMGNPPHDDE
                     YLELHVRNMANGAFTEYVFNKMKEKDILRFEGPLGTFFMREDSDKPIVMVASGTGFAP
                     IKSIIEHAFHKSTARQMVLYWGGRRPADLYMAEIAGQWQQQHDNFTFIPVVSDATADD
                     AWQGRTGFVHKAVMEDLPDLSGYQVYACGAPVVVESAHKDFTTQCGLPEDEFYSDAFT
                     PSVDPKPAPA"
     gene            complement(6617..9382)
                     /gene="ppc"
                     /locus_tag="VN496_15125"
     CDS             complement(6617..9382)
                     /gene="ppc"
                     /locus_tag="VN496_15125"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXF18338.1"
                     /translation="MDALILKPDATDKESPLREDIRLLGRILGDTLREQEGEATFDLI
                     ERIRQNAIRFRRDRDPDAKHELDAMLNKLDPVRAIAVVRAFSYFSQLANIAEDQHHNR
                     RRRAHLIAGSGPQEGSMALALSRAGDEKVTAAQVSGFFSQALIAPVLTAHPTEVQRKS
                     ILDCQLEIARLLTERDRTQLTPEERVHNEEALRRVVLTLWQTRILRELRLTVNDEIEN
                     GLAYYRYTFLHQLPKLYAEMEDLLAEHGFAKTKPGNFLKLGSWIGGDRDGNPYVTDDV
                     MRHALARHSATALDFYFEELHKLGGELSQSVRIVETTPALDELAGNSPDVSEHRRDEP
                     YRRALTGIYARLSATAHRLGHRLSGRPAVAVAQPYTDQAEFSKDLAILADSLAANGSK
                     RIARGRLRTLVRAAEIFGFHLASLDMRQHSGVHEQVVGELFLLGARKAGYAELNEKER
                     RQWLLQELAVPRLLRSPYLTYSQDVSKELAILDTAAQLHREFGAASLPNYIISKADSV
                     SDMLEVALLLKEAGLLHPGEHPRLELNIIPLFETIEDLQGCGAVMEQLFCEPYYRELL
                     ASRGGMQEIMLGYSDSNKDGGFLTANWELYKAEVELVKVFARHGVKLRLFHGRGGSVG
                     RGGGPSYQAILAQPPGSVAAQIRITEQGEVIASKYSDPDIGRRNLETLVAATFEASVL
                     RTESAGELAFVEVMDEMSKTAFVAYRKLVYETPGFVQFFRSATPITEIADLNVGSRPA
                     SRKNSDRIEDLRAIPWVFSWSLSRIMLPGWYGFGTAVEELLSRRGEQAMAVLRRMYRD
                     WPFFRTLLSNMDMLLAKSDISIASRYAELVSDLDLRESIFAKIQHEWHQTVRHLLAIT
                     GQNELLEANPALSRSLRNRSPYIDPLNHLQVELLRRYRAGEMDERNRRAILLTLNGIA
                     AGLRNSG"
     gene            complement(9552..10760)
                     /locus_tag="VN496_15130"
     CDS             complement(9552..10760)
                     /locus_tag="VN496_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HXF18339.1"
                     /translation="MRALVWAIGLFAVAVGLVIAARYNSGYVLIVLRLWRAEVSLNFA
                     IALACACFILVYILVRTIIATATMPSRVRAFQKRRSEARARASFNEALTNFFEGRFGR
                     AEKAAVAALKAGESPALSSLLAARAAHGMRAFATRDKYLTLATGLDVHGDSMKVMAQA
                     EMLLDERRYFDALDVLKKLPEKHTAALRLELRAQQMAMNWERVLALIPQLEKRRVFER
                     PVVAQLRRQAVIESLKRKAVDVVSLREYWERLSPEDRYEPRVAANAAQAFIILDACGD
                     AHRIVEEGLARHWDPALLAMYSECLGGDVRQQLERAELWLQHHPRDAVLLLVLGRLCS
                     HQGLWGKARSYLEASLSIEPTHSAHLELGRLLERESKPAEAAAEYQKALRVTLEQLKQ
                     YGGGRRRPIV"
BASE COUNT         2502 a         3129 c         2898 g         2409 t
ORIGIN      
        1 cggcgcctcc tcgatgatct tctgatgtcg tcgctgcatc gagcaatctc gatcaccgag
       61 atagacggca ttgcggtgtt catccgcaag cacctggatc tcgacgtgtc gcggagtttc
      121 caaaaatttt tccatgtata ccgttggatt gccgaacgcg ctcttagctt cctgccgagt
      181 cattgccact gcattgacca gcgcagcttc ggtatgcacg actcgcatgc cgcgcccacc
      241 gcctccaccg gccgccttga taatgacggg atacgcaatt ttccgcgcaa ttttgacgat
      301 ctcttgaggc tcttccggca acgccccttc gcttccgggg acgaccggca cgcctgcctt
      361 tatcatcgta ttttttgcac ttaccttgtc acccatcagc cgaatggtgt ctgggcgagg
      421 accgataaag acaaacccgc ttttttccac gcgttccgcg aaatcagcgt tctccgacag
      481 gaagccgtag cccgggtgaa ttgcttcggc atccgtaacc tcggcggcac tgatgatggc
      541 cggaatattg agatagctat ccgcagaggc cgctggaccg atacagaccg attcgtcggc
      601 cagcctcaca tatttggcct ctgcatcggc cacggaatgc acggcaaccg tcttgatccc
      661 gagttcccgg caggcgcgtt gcacacggag ggcaatttca cctcgattcg caatgagtat
      721 tttttcaaac atgggcgttc tgctaaggct gtgcggcggc tcgacgcttt gtcggggccg
      781 ggtaccctct tacccgatga ggaacaatgg ctggccgtat tcgaccggtt gcccgttttc
      841 gaccaggact ttcttgacga cacccgtctg gtcggcttct atctcgttca ataatttcat
      901 cgcctcaatg atgcaaagcg tttcacctgc attgatggtt tggccgatct ccacaaatgg
      961 cttggaccca ggcgcagatg agcgatagaa cgttccaacc atgggggact taacaacatg
     1021 gccctctggc tcggtagcct caggcaccga aggtgcagca agaggcgcgg ccgttgggct
     1081 ctgcgctgca aggatgggag gttgcggcaa agccaccgcc gctggatgtc cttgaacgcc
     1141 gatacggttg attcttactt tttcctctcc ttcggtaatc tcgagctcgg caatgcccga
     1201 ctcctgtacc agatcgatca gtttcttgag ttttctaagg tccatcgcaa ttcctatttc
     1261 gacggcgtgt aagccattgc atgggccaat gcatattcat agcctttcga ccccaaaccg
     1321 caaattgtgc ccactgccag atcgctgaag taagacttgt gccggaatgg ttcccgtgca
     1381 tgaacgttag agagatgcac ctctatgaac ggaatgcgta ccgcagcgag cgcgtcccta
     1441 agtgccacgc tcgtatgcgt aaatgccgca ggattgataa caataaagtc cacccccgtc
     1501 agccgagcgt tgtgaactct gtcaattagt tcagattccg aattactttg atacgtctca
     1561 agtacaatcc cggccccggc cgcttggctc acaagtctgt cgtcgaggtc cttaagtttt
     1621 tcccctccat aaatttgcgg ctcacgggta ccgagcaaat tcaggtttgg accatgcaat
     1681 acgagaactt tgcgggactt tgatgccggc gaaggacgca ctttgccacc cttcaaggtg
     1741 gtagtcatgc cagccagttt gccgtagtta gcaggttttt gtccacattt aagcgaaaat
     1801 cgtcccaacc agcatgctgt tggaatgatt tggctaacag cgctcggtcg aggtgtaagc
     1861 ccggctactt gaagtgctca agatccgcaa ggacggccag aaagcgctcc gccggctggt
     1921 acccaatcac ccgcaaccct tctaattcct tgccgtggcg atcaaaaaaa ataacgcccg
     1981 gagggccgta caatccgaat cgctttagaa gggcttcgtt ttgagcggtg ccatccgtga
     2041 catcggctcg cagtagcagc atgttatcca gtcttctttt gacatttgcg tctcgaaaag
     2101 tcaggcgctc catttccttg caagcgacac accagtcagc ataaaaatca accatgattg
     2161 gctttccggc ccgaataaca gctgcttcca gttcagcgat cgaggccacg ggaataaagg
     2221 ccggaccttc caagtccttt tggccggttc gcgtttgcag gccagacaat ggttgcagaa
     2281 tgtcccgtcc gccggacagt ccgcctacca ataggacgat gccgagcaac agagcaatca
     2341 ctccaatacc cttccacagt ctccgcgagc cgctggctcc agggggcagc gggtctatcg
     2401 cgtgcagata gatgccagac aggatcaaaa gtaccgacca gcacaacatc tgtaccgtca
     2461 cgggaatgag cggagcgatt atccagatcg ccccagcaag cagcaggaca ccaaaaaagt
     2521 tttttacggt ctgcatccat gcccctgcct tgggcaggaa tgtaccggct gacgcgccga
     2581 ggaccagcaa aggtatgccc ataccaatac ccatcgtgaa caatgccagg cctcccagta
     2641 ccacatcatg cgattggctg acatagagta atgcgccggc cagcggagct gctacgcaag
     2701 gcccgacaat gacggccgaa attgcgccca tgccgaaaac gccgcccaag gtgcctgccg
     2761 tcagacggtt actggtatcg gcagcgccgg tttgccatgc cacgggaagt tgcagttcgt
     2821 acaaaccgaa catcgataac gcgagtaata cgaaaatggc tgcaaaactg atcaaggccc
     2881 atggtgtttg caatgccgca gacaacagcg tcccggacaa accggcaatg acgccggcaa
     2941 ttgcatagga gagggccatg ccgagaacat atatcgcaga gagcgtggct ccgcgcatac
     3001 gggtgatttt ctttccctgg ccggcgatca gtccagacag aattggaacc ataggcaaca
     3061 cgcagggggt cagcgaaaga agcaacccga atcccagaaa actgcccatg atgagccaga
     3121 aattacctgt tttcatgaac cgggcaattt tcgcatcctc agccggctcc ctggtgaagg
     3181 tgcctggaga ctgcaacgat gttgaaccat tcgcggacaa accgccggga tccgcagttg
     3241 agaatggcac atacgttacc ggcaactgac gaatcgctgg cgggtagcac agtccttttt
     3301 cgctgcagcc ctgaaacccg acatttacag ccaatgtcgt ctgttcttga atggcttctc
     3361 gcagtcgaat aatcacttgc actgggacac ggaatatttc cgttttccca aaattcggat
     3421 cctgctttgt ttcccctcgc gggatctgca ccgtgtcgat cgtggcgatg gtgccgggta
     3481 cagtcttgac ttgaatcttg tcacggtaaa ggtaataacc atccgccggg ctaaggcgcg
     3541 ccatgagggt ttgtgcatca gcgaagcgta cttcaacgtt aaacgccttc tccaccggga
     3601 ggaattcttg ctcgtcagca gctggtccag tcccattctg aagccgtgcc agcaggccag
     3661 ctggtttccg cggagagggc aatccattgt caatagtcga tgccggcgtc gcgggctcaa
     3721 ttcgtacggt ttgcgtcaac ggcatgtagc acacaccgat gtcagcacaa ccttgagaaa
     3781 caaccttcaa cgtcagtccc gccgctggaa gtgatgactt gagtggcaga tcgatagcca
     3841 catcaccgcg gtaaattggt actctgccaa aaaattcgtc atctttgata gtaccggccg
     3901 gcaaatccgg cgtgcccaac tttacaggcc cttctgctat ggcaaagtgg aaacgatccc
     3961 ggtataggta gtacccatcc gcgatctgat aacgcacttg aatggatttt tcgtccaagc
     4021 gactggccga gacgcggaag gcagtgtcgg gcgcaagcag gccgtctgca gcccaaccgg
     4081 gcgtgaattg cggcgaagcg agtaaggtca gcaataagaa gaatcgagca ccggggtggt
     4141 tcatatgcgg aatagaaatt gaccccggtt ctaacggacc gcagattccg tattcaccca
     4201 atccagatag gccggcagtc ctttggccac tggaatgccc aagatttccg gaacctcgta
     4261 gggatggttg tcacggattt tttcctcaag acgcgcgtac tccgataatg tcgtcttgat
     4321 cagtaaaggc acttcagtcg ccatattgac ttcaccattc cagcgataaa ccgaggtaca
     4381 ttcggcgagg atgtttacgc acgcggcatg gcgatgttcg atcagcaatg ctgccaactt
     4441 ggttgcggaa gtacggtcgg gcagattggt caaaacaata ataagatcgc tgcgggtatc
     4501 aacgcccatg agtcctccaa aagcacagcc accacgctct ttcatcgatc acagtgtttt
     4561 catcgcatcc catgccgcca caacaccgtg ttgcacagaa gggtaccgca agcgcacacg
     4621 caactccttt ttcaatctgt cattcgcaag tctgcgggat tcgcgcataa atgaaagcaa
     4681 ttgttcaggg agttcgtttt gtgcgatctc ccagctcact ctaggaggtc ggggaagatt
     4741 gaattgttcc gcaacgatgt caaagtaatc tcccatcttc agccagcaat catcggaagc
     4801 gttataggcg cgtccgcctc gtccccgaac gagcgccgcc aacagaatgc gggccaggtc
     4861 atccgcatgg atatggttgg tataaggatc acgttccgaa gaaagcgagg gcatgccgga
     4921 ctgcaggcgc gccagcggca ggcgcccggc gcagtaaatt ccaggcacgc gaaggatgct
     4981 gacctgcacg cctgtttcgg tgccccagat ccggatttgc ttttcagcat cgagcctgcg
     5041 cctggctctg gtagtggacg gctgcgcagg acgcgtctcg tatacgactt caccggcgca
     5101 atccccatac acgccgctgg tactgatgta tatgagacgc tgtggtagac tgccgccctt
     5161 cgcgagcgcc ctgatcaggt gagtggttct ggtatcgcga tcgccagaag ctggcggtgg
     5221 tgcaagatgc aagatgtcct gcgccaatgc ggcaattctg tgcaacgtat caggatggtc
     5281 caggtctcct gataccggcg ttatacctgt ggtgcgcagt tctgaaaatc gagcctcagt
     5341 atgggtaagc gcgtaaatac gccaacggcc tttcagcgct ctggccagac gcagcccgac
     5401 atctccgcaa ccaatgatca ataatcgcct catccttata ttttaccccg gaccaaattg
     5461 ggtctcgctg gcctattgat gtcatgtctc accaggtaac catcaaaccc agcgaccata
     5521 ccttcatctg caccgatgaa gaatccgtgc tggccggcgc attacgcgca ggctttacgt
     5581 tgccttatgg ctgccggaat ggagcctgtg gtgcttgcaa aggtaaaatc ctccagggca
     5641 gtgtggacta cggtaatccg cagccttatg ttcttgccga attcgagaag aaggctggac
     5701 tcgcgctgtt ttgccaagcg cggccattga cggatctggt aatcgaagcg cgcgagatac
     5761 gcggcgtcgg gcagctggac attcgcaagc tgccttgccg ggtacaaacc attcaccgcg
     5821 ttgcgccgga cgtcgtagtc ctgaaattga aattacctgc caacgaacgc ctgcaattcc
     5881 tggcgggaca atacatcgac atcttgatga aagacggcaa gcgcaggtcc tattcgatgg
     5941 ggaacccgcc acatgacgac gaatatctcg aactgcatgt caggaacatg gcaaatggcg
     6001 cttttacgga atacgtcttc aacaagatga aggaaaaaga catccttcgt tttgaggggc
     6061 ctttggggac cttttttatg cgcgaggatt ccgacaaacc catagtgatg gttgcaagcg
     6121 gcaccggttt tgcgcctatt aaaagcatca tcgaacacgc attccataag tccaccgcgc
     6181 gacagatggt gctgtactgg ggcggccgcc gcccagccga cctctacatg gcagaaattg
     6241 ccgggcagtg gcaacaacag cacgataact ttacattcat tccggtcgtc tcggatgcca
     6301 cggcagatga tgcatggcag gggcgcactg gattcgtcca caaagccgtc atggaagacc
     6361 tgcccgatct atcggggtac caggtatatg cgtgtggagc accggtagtt gtggagtccg
     6421 cacacaagga tttcacgacg cagtgcgggc tgcctgaaga cgaattttat tctgacgcgt
     6481 ttacaccttc ggtcgaccct aagcccgctc cagcatagtt ggcgcaagag cggccgaagc
     6541 tgtcggctaa ggtaagcgga cacacctgcc acgcgccgga aaagcgcaca accgctgcct
     6601 gccagtcatg tagtggctat ccgctattgc gcaaaccggc ggcgatccca ttaagggtca
     6661 gcaaaatcgc gcggcgattc cgttcatcca tttcgccagc ccgatagcgt ctcagtaatt
     6721 cgacctgcaa gtgattaagc gggtcgatgt acggcgatcg attccgcaag cttcgggata
     6781 acgcgggatt agcctcaagc aattcattct ggccggtgat cgcaagcaaa tgacgcacgg
     6841 tttggtgcca ttcatgctgg atttttgcaa atatgctttc tcgaagatcc agatcgctca
     6901 ccagttccgc ataacgtgaa gcgatgctga tatcgctttt cgccagaagc atatccatgt
     6961 tggataacaa cgtgcggaaa aacggccagt cgcgatacat gcgtcgcagc acggccatcg
     7021 cctgttcccc gcgacgtgac agcagttctt cgacggcggt gccaaatcca taccagccag
     7081 gcaacatgat gcgcgacaag gaccaggaaa atacccacgg gatagcccgc agatcttcta
     7141 tgcggtccga attcttgcgg gaggctggcc ggctgccaac attcaaatct gcgatttcag
     7201 taatcggggt tgcactgcgg aaaaattgca caaacccggg cgtttcgtag actagttttc
     7261 ggtaggcaac gaatgctgtc ttgctcattt catccatgac ttcgacgaac gccaattccc
     7321 cggcgctttc cgtccttagc acggaggctt cgaatgttgc cgcgaccagc gtttcgagat
     7381 ttcggcggcc aatatccgga tcggaatact tgctggcgat aacctcgcct tgctcggtga
     7441 tgcggatttg agcggctacg ctaccaggcg gttgggccag gatagcctga tagcttgggc
     7501 cgccgccacg accgacggag ccgccgcgac catgaaaaag tctgagcttt acgccatggc
     7561 gcgcaaatac tttgaccaat tcaacctcag ctttgtatag ctcccaattg gccgtcagaa
     7621 atccgccgtc cttattgctg tccgaatatc cgagcatgat ctcctgcatg ccaccgcggc
     7681 ttgccagcaa ctcgcgatag taaggttcgc agaacagttg ctccatgacc gcgccacaac
     7741 cctgcaagtc ttcgattgtc tcgaataacg gaatgatgtt cagctcgagc cgcggatgct
     7801 cgccaggatg cagtagaccc gcttccttga gcaggagagc gacttccaac atatcgctta
     7861 cgctatccgc cttcgatatg atgtagttgg gcaacgatgc cgcgccgaat tcgcggtgca
     7921 actgggccgc tgtatcgaga attgccaact ccttgctcac atcctggctg tatgtcaggt
     7981 aaggcgagcg cagcagtctc gggaccgcaa gctcctgaag caaccattga cgccgctcct
     8041 tttcatttag ttcggcataa ccagccttgc gtgcgccaag caggaacaat tctccgacga
     8101 cttgttcatg cactccgcta tgctggcgca tgtccaagga ggcgagatga aagccgaaaa
     8161 tttctgcagc ccggacaagc gtgcgtaatc tgccgcgcgc aatgcgcttc gacccgtttg
     8221 cagcaagcga gtcggcgagt atcgcaagat cttttgaaaa ttcggcttga tccgtataag
     8281 gctgcgcaac agccaccgca ggtctgccgg ataagcgatg gccaagccga tgcgcggtcg
     8341 cagaaagacg ggcatagatt ccggtcaatg cgcggcggta gggctcgtct cggcgatgtt
     8401 ccgagacgtc aggcgaattc cctgccagct catcgagagc tggcgtcgtt tccacaatgc
     8461 gtacagattg cgaaagctct ccgcccagtt tatgtagctc ttcaaaatag aaatccagcg
     8521 cagttgcgga atggcgggct agcgcgtggc gcattacatc atccgtgacg taggggttcc
     8581 cgtctctgtc accccctatc cagctcccca gcttgagaaa atttcccggc ttcgttttcg
     8641 caaagccatg ctccgcaagg aggtcttcca tttcggcata gagtttcggc aattggtgca
     8701 gaaaggtata acgataatag gcaaggccgt tctcaatctc gtcgttcacc gtaaggcgca
     8761 gttccctcaa aatacgggtt tgccacagtg ttagcacaac gcggcgaagt gcttcctcat
     8821 tgtgcacccg ttcctcggga gtcagctgag tacggtctcg ctctgtcagc aagcgcgcga
     8881 tttccagctg gcaatcgaga atgctcttgc gctgaacctc ggttggatgc gcggttagca
     8941 ccggtgcgat caaggcctgg gaaaagaatc cagaaacctg cgccgccgta accttttcgt
     9001 cgcctgcacg gctcagagcc aaagccatgc tgccttcctg cggaccggag ccagcgatca
     9061 agtgcgcgcg acgacgcctg ttgtggtgtt ggtcctcagc aatgttcgcc agttgcgaga
     9121 agtaactaaa tgcgcgtaca actgcgatgg cgcgaacggg atccagtttg ttcagcatgg
     9181 catccagttc gtgttttgcg tccggatcac gatcgcgtcg aaaacgaatc gcgttctgac
     9241 gtatccgctc tatgagatcg aacgttgcct cgccttcctg ttcgcgaagc gtatcgccaa
     9301 ggatgcgacc cagcagccga atatcctctc gtagcggaga ttccttatct gttgcatccg
     9361 gctttaggat tagggcatcc atttagtcgc taatcgggat tgaaaacgga acgaagcgca
     9421 agttggaccc tgaagcaagc cttgcgccgg ggtcgccagc gcttcaattt tttcatttgt
     9481 tcaacggttt aattaaaacg acaccacacg aaaccgcgct aacaggcaca gcgatggtgc
     9541 gcaaggcaca tttacacgat cggtctcctg cgccccccac cgtattgctt caactgctct
     9601 agtgtgactc tcaacgcctt ttgatactcg gctgccgcct cggccggctt actttcgcgt
     9661 tccaacaatc gtccaagctc cagatgtgca gaatgcgttg gttcgataga cagactcgct
     9721 tcaagataac tgcgcgcctt tccccatagt ccctgatgcg aacacagccg tcccaatacc
     9781 aatagcagga ctgcgtcgcg tggatggtgc tgtaaccaca gttcagcgcg ctcgagttgt
     9841 tgtcgaacat ccccgccaag gcattcagaa tacattgcaa gaagggctgg atcccagtgg
     9901 cgcgccaggc cttcttcgac gattctgtgt gcatccccac aggcatcgag aataatgaag
     9961 gcctgcgctg cgttcgccgc aacgcggggc tcatatctgt cttccgggga aagcctttcc
    10021 caatactccc gcagcgatac aacatccact gctttgcgtt taaggctttc aatgacagcc
    10081 tggcgtcgta actgggcaac gaccggtcgc tcaaatactc tgcgcttctc cagttgggga
    10141 atcaaagcga ggacccgttc ccaattcatt gccatttgct gcgcacgcaa ttcgaggcgc
    10201 agcgcagctg tgtgtttctc cggaagcttt ttaagcacgt caagcgcatc aaagtaacgt
    10261 cgctcatcca gcagcatttc tgcttgcgcc atcaccttca tggaatcgcc atgtacatcc
    10321 aagccggtgg caagggtgag gtatttgtcc cgcgtcgcga atgccctcat gccgtgagca
    10381 gcgcgagcgg ccagcagaga agacagggcg ggagactctc cagccttcag cgctgccacg
    10441 gccgccttct ccgcgcgtcc gaaacgacct tcgaagaaat tggtgagcgc ttcattaaag
    10501 ctggcccggg cacgcgcctc ggaacgccgc ttctgaaatg cccgaactcg ggatggcata
    10561 gtagcagtgg ctattatggt gcgcaccagg atgtaaacga gaatgaaaca ggcgcaagca
    10621 agtgcaatgg cgaaattaag cgacacctcg gctcgccaaa gacgcagcac aataagtaca
    10681 tagccgctgt tgtagcgtgc cgcaatgacc aatccgacag cgacggcaaa tagaccgatc
    10741 gcccaaacaa gggcccgcat caattcacct cgccgtattc agcatgcatc ggatcagggc
    10801 aaggtacgct ctcgtaccag cttcaggttc cgagcagcat caaggctatc cgaaatatcc
    10861 ggcaattgag cacccacggg ggactggagc aattgcttca gatttgattg tgccgtcatc
    10921 acgcccttgt tgccgcca
//
LOCUS       DATMZQ010000475         3697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4401760, whole genome shotgun sequence.
ACCESSION   DATMZQ010000475 DATMZQ010000000
VERSION     DATMZQ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4401760"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VN496_15135"
     CDS             complement(<1..1712)
                     /locus_tag="VN496_15135"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HXF18340.1"
                     /translation="MQPGKSYRAAVASGALFMFVYPAGAEAAEELEDESHGFRPSISH
                     ELSAGGISRIGQRDPGLVGRANGGTGTSVNFDDGNLNYGRGLASFAVQGRSIVAGGSD
                     RVEVGLEAVYFYDFVNADGHTDFHNLSEEARIRAGRNIYVNEAFVGLKQRVDEAMLSV
                     RAGNQRLRWSDSAWFGQSIAPVNAVAASRRYQPGNTAKDMVVAVPMIFGDLVTASHWT
                     FSGFYQFDFKPTEPEAAGTFLSANDYYSPGSRYLQLGQGSPLVPDQDVSVTTSATPFG
                     SRVPRGEDRLPDSSGQYGLRVETPELGASQFALAGYAMRVHSREPIVSVHTGTLEGLL
                     GITAPDYTSSGNYFIEYPPDVTILGTSARLKPASFTQLSVNYSMRIGQPLQIDDEILI
                     TAGLAPAAATAVCGPAPASALCAGTLATLNRNPLIASRGGITASNAGAFFDKELSGFE
                     RFDVSQWAVSAVQGLPPVFGATQWFASAEAGGVYVHGFKPGFLDASVTVRPDENGARR
                     NGFASRSAWGYRLFSRIEFPNVAGLRSVAPSLTWIHDVQGNAPITLGTVLEGTMSYIL
                     ALEAA"
     gene            complement(1888..2751)
                     /locus_tag="VN496_15140"
     CDS             complement(1888..2751)
                     /locus_tag="VN496_15140"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HXF18341.1"
                     /translation="MRNIPTSMLDKAAQALGEPPSFLAGIGRCGHAAATPTNFCHIHI
                     HFLGQFQIFRGGRRLALGRKKPKRPLDLLKILIASGGLRADTARLSESLWPDSDGDAA
                     RNCFDITLHRLRQILSIPGLLSLSEGKLSFDATNCWIDVWAFENVVQRIEALTMSSTH
                     CDLQDHDPLLADLLRLYAGHFLEHENQEVWAAAFRDKLKAKFGRSVINLAVCLERQRK
                     WDQAAVLYSRALELDNLAEPLYRRLMICYRELGETAEALKTYRRCQELLSIVLCVRPS
                     AETEAIRESCR"
     gene            complement(2769..3428)
                     /locus_tag="VN496_15145"
     CDS             complement(2769..3428)
                     /locus_tag="VN496_15145"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXF18342.1"
                     /translation="MPTRRLAAILCADAIGYSRLMQDDDQHTLETLKRYRACIAKICL
                     VHDGRVVNTPGDSVLVEFSSAVEAFAAAVEIQDSIESRNGQLELQRRMYFRIGLDLGD
                     ILVDSDGSVYGNGVNIAARMQALAGHGGVVMSGRIFDEVSARLHYRFHALGRRRVKNI
                     RDRVRAYEIRSAAGGTISRRNSAQHLAEILRGLLIAAANYLFASAGSPGAHGALAPRT
                     E"
BASE COUNT          786 a         1113 c         1077 g          721 t
ORIGIN      
        1 gcggcttcga gcgcgaggat gtaagacatg gtgccttcca gtaccgtccc cagcgtgatc
       61 ggtgcattgc cctgcacgtc atgaatccag gttagcgagg gtgcgacgct gcgcagtccg
      121 gcgacgttgg ggaattcaat gcgagagaac aatcgatatc cccatgccga gcggctggca
      181 aacccatttc ttctcgcgcc gttctcgtca ggccggaccg tcacgctggc gtccaggaag
      241 ccgggtttga atccatgtac ataaactccg cccgcttctg cgcttgcaaa ccactgcgtg
      301 gcgccgaaca ccggcggcag cccctgcacc gcagacaccg cccactgcga aacatcaaaa
      361 cgctcgaagc cacttaattc tttatcgaag aacgcgccgg cgttacttgc cgtgatgccg
      421 ccgcgtgagg caatcagggg attgcggttc aacgttgcca aggtgcccgc gcagagtgcg
      481 cttgccgggg caggcccgca cactgcggtt gcagctgccg gcgccagccc tgcggtaata
      541 aggatctcgt cgtcgatctg cagcggctga ccaatgcgca tcgaatagtt cacgctcagt
      601 tgggtgaatg atgcaggctt cagccgggcg gaagttccca ggatcgttac gtcaggcggg
      661 tactcgatga agtagttgcc gctggacgtg tagtccgggg ccgttattcc gagcaggccc
      721 tcgagggtgc cggtgtggac gctgacgatc ggctctcgcg agtgcacgcg catcgcatag
      781 cctgcaagcg caaattggga ggcgcccaat tccggcgtct caacccgcag gccatactgc
      841 ccggaagaat ctggcagcct gtcttcgccg cgcgggacgc gggagccaaa gggcgttgca
      901 gaggtcgtca ccgacacatc ctgatccggc accagcggcg atccttgtcc caactgcaga
      961 taacgcgacc ctggagagta atagtcgttc gcgctaagga aagtaccggc cgcttctggt
     1021 tcggtcggct tgaagtcaaa ttggtagaag ccggaaaacg tccagtgact ggcggtgacc
     1081 agatcgccaa aaatcatcgg cacggcgacc accatatctt ttgcggtatt gccaggctgg
     1141 tagcgccgcg aagcagcgac cgcattgacc ggcgcgatgc tctgcccaaa ccatgcgcta
     1201 tcgctccagc gcagccgctg gttgccggcg cgcacgctca gcatggcttc atcgacacgc
     1261 tgcttaagcc cgacaaaagc ttcgttcaca tagatgttgc ggccggcgcg gatgcgtgct
     1321 tcttcgctta ggttgtggaa gtcagtatga ccatcggcat tgacaaaatc ataaaagtaa
     1381 accgcttcca gtccgacttc gacacgatcg gagccgccgg ccacgatcga cctgccttgt
     1441 accgcgaacg acgccagacc gcggccatag ttaaggttac cgtcgtcaaa attcaccgac
     1501 gtcccggtcc caccgttggc gcgaccgacc agcccgggat cccgctggcc gatgcggctg
     1561 atgccgccgg cggacagttc atgcgaaatg ctggggcgaa agccatggct ttcgtcctcg
     1621 agttcctcag ccgcctccgc tccggcaggg tacacgaaca tgaacagcgc tcccgaagcc
     1681 acggcggcgc gataactttt acccggttgc atggggctcc ttgctttgaa ggcaaaaggc
     1741 aggagacatc ttcatgttat cgcgccgctg gcgatcagca gcgagctatt gcgcgatgtc
     1801 gaaattacga tcattgaccg ctccgtccgc tgcaagaaag gtatcggcac ttgccttacc
     1861 atgagcatac gcattggcaa gtgcacttca tcggcaactc tcgcgaatgg cctctgtctc
     1921 ggccgaaggc ctgacacata gaacgatcga cagcagttct tggcagcggc gataagtctt
     1981 cagtgcctcg gccgtctccc caagctcgcg atagcaaatc atcagccggc gataaagcgg
     2041 ttcggccagg ttgtccagtt ccagtgcccg ggaatagagt accgcagcct gatcccattt
     2101 gcgctggcgc tcaaggcaga cagcaaggtt tatgaccgac cttccaaatt tcgctttcag
     2161 cttgtcgcgg aatgcggctg cccagacttc ctggttttcg tgctcgagga aatgcccggc
     2221 atacaagcgc agcagatcgg caagaagcgg atcgtgatcc tgtaggtcgc agtgcgtgga
     2281 cgacatcgtc aacgcttcaa tccgctgcac cacattttca aatgcccaga catcaatcca
     2341 gcaattggta gcgtcaaagg aaagctttcc ctcggacaat gacagcaagc ccggtatgct
     2401 cagaatctgc cgaagccgat ggagtgtgat gtcgaaacaa ttcctcgccg cgtcgccatc
     2461 ggagtcgggc cacaagctct cggacaagcg cgccgtatcc gcccgcaagc cgccactggc
     2521 gatcaggatt ttcaacaaat ccagcggcct tttgggcttt ttgcggccaa gtgccaagcg
     2581 ccggccgccg cgaaaaattt ggaactgtcc aaggaaatgg atatggatgt gacagaaatt
     2641 ggtcggcgtt gcagcagcgt gcccgcaccg gccaatgccg gcgaggaaag acggcggctc
     2701 gcccaatgct tgggctgcct tgtctaacat cgaagtcggg atgtttctca tggcgtggtc
     2761 aggcacgatc attctgtgcg gggcgcaagt gcaccatgtg cgcccggtga cccggccgaa
     2821 gcgaatagat aattcgctgc cgcaatcaac aggccgcgca gaatttcggc aaggtgctgg
     2881 gcagagttgc gcctggaaat tgtgccaccg gcagcactcc tgatttcgta agcgcgcacc
     2941 cgatcgcgaa tattcttcac gcgccgccga ccgagcgcat ggaaacggta gtgaagacgg
     3001 gcagacactt catcgaatat cctgccggac atcacaacac cgccgtgacc agcaagcgct
     3061 tgcatgcgcg ccgcgatatt cacaccgttt ccatacacgc taccgtccga atcgaccagg
     3121 atgtctccaa ggtcgagccc aatgcgaaag tacatccggc gctgcaactc cagctgaccg
     3181 ttgcggcttt caatgctgtc ctgaatttcg acggcagcag caaaagcctc taccgcactg
     3241 gagaactcaa ccagaaccga gtcgccgggc gtgtttacaa ctcggccgtc atgcacgagg
     3301 catatttttg caatgcaggc tctgtagcgc ttcaatgtct ccagcgtgtg ctggtcgtca
     3361 tcctgcatca ggcgggaata gccgatcgca tctgcgcaaa ggatggctgc gagccgacgc
     3421 gttggcattg caggcgggga aaaccactgc ttccgtgcgc tcggcctgtt catccgatga
     3481 tgacggaatg ggtggccgtc ctgcgattgg gcacgagccc gatccacgcc gtttttttcg
     3541 atacatgcga cacgagaaac catctctgct gacgtgttct cgggcagttc cgaagcgagc
     3601 gcagcgaaat cggcgagggg cctcatattt atcgctattc gctggcgcca gccgcggcgc
     3661 gatcttgagc aaaggtggaa aagtcggggc gcagtgg
//
LOCUS       DATMZQ010000476        10588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5047743, whole genome shotgun sequence.
ACCESSION   DATMZQ010000476 DATMZQ010000000
VERSION     DATMZQ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5047743"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VN496_15150"
     CDS             <1..482
                     /locus_tag="VN496_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18343.1"
                     /translation="GDDQQANTHVGGVNATTPAGSLNNGQINGAWNTATNTDESCLFW
                     SHIRMANLGPGATTPACNAAGYYPVNASGGQIGVQSSNGFTTITLPTPMTGTYVICSA
                     NIPGEYVIQLDTLLDDGDPQTGSMRAIDQSALAAATAATIAGVQANLGGNYTVCLSV"
     gene            complement(509..889)
                     /locus_tag="VN496_15155"
     CDS             complement(509..889)
                     /locus_tag="VN496_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXF18344.1"
                     /translation="MSDHDHRPPIGVAHVVLETNRMQESASFMKTIGMRPIFEGPEVG
                     VYELRGGTHLILMLKNSIVPGNAQFDLMVDDLHATHQRFASQGLAPSPIESRPAIGHE
                     VFTVREPAGHAITFFSSHVSGKPV"
     gene            complement(954..1538)
                     /locus_tag="VN496_15160"
     CDS             complement(954..1538)
                     /locus_tag="VN496_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXF18345.1"
                     /translation="MGKGRLEAFSDGVIAIIITIMVLEMKVPHGESLESLLPLLPVFM
                     SYVLSFVYVGIYWNNHHHMLHATGRVSGTVLWANLHLLFWLSLFPFATGWMGENHFAP
                     MPSALYGAVLLMAAIAYWILEQALIASEGSASVLKTAVGKDWKGKLSPLFYVIAIAST
                     FWVQWVAQAMYVLVALIWLIPDRRIEHVLQRRET"
     gene            complement(1563..2210)
                     /locus_tag="VN496_15165"
     CDS             complement(1563..2210)
                     /locus_tag="VN496_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXF18346.1"
                     /translation="MSKLRVQSFSVSIDGYGAGPNQDLQNPLGVRGTELMEWFFPTRV
                     FRRMYGDGDGETGTDNAMAEQGFANIGAWILGRNMFGPVRGPWPDENWKGWWGEEPPY
                     HTPVFVLTHHPRPPLRMAGGTEFRFVTQGIRAALEQATAAAKGLDIRLGGGVTTIREY
                     LRAGLIDELHFAIRPVLLGSGEHLWNDIDMHALGYECQRYVAGERAAHVFLGKRS"
     gene            complement(2448..3275)
                     /locus_tag="VN496_15170"
     CDS             complement(2448..3275)
                     /locus_tag="VN496_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXF18347.1"
                     /translation="MKADFPPATFRDFEHQAWQRVVKLYEDHFGRLTAQSIEPLLNAV
                     NVHSGDSVLDVATGPGYIAAAAAQRGAEITGLDFSSAMISEAAALHPGIHFREGDAGD
                     LPFQDASFDVVVIGFGILHFPDPDRALREACRVLRSGGRFGLSVWAGPDKAVGFGIIM
                     RAVEKHGSMNVGLPPGPPFFRFSDAAQCSQAIAGAGFKSVTITEVPQTWHFASATAFF
                     DGISASTVRTGALLRAQSPKNLANIRAEVEREANRYGAAGGGIALPMPALIASGRKS"
     gene            complement(3301..3723)
                     /locus_tag="VN496_15175"
     CDS             complement(3301..3723)
                     /locus_tag="VN496_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXF18348.1"
                     /translation="MASYIDQVLINDERVLYRARLSKWSLWPQIVFGALLVPLFGIGL
                     LLWLWAWILYATTELAITNKRVIAKTGLIERRTTEMFLEKIESIQVNQSIFGRLFDFG
                     SVVISGTGIHSAPFKNISDPLALRRNFMMAADARQRTS"
     gene            complement(3794..4072)
                     /locus_tag="VN496_15180"
     CDS             complement(3794..4072)
                     /locus_tag="VN496_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18349.1"
                     /translation="MLKLLPFVLFMICALARAGTPDATPTNDWLAMGPDPGGHTGARV
                     TPETTKTDRPEVDAARKAASGSKTESNTPAPAEKGQSKSENKSSKPNN"
     gene            complement(4189..5985)
                     /locus_tag="VN496_15185"
     CDS             complement(4189..5985)
                     /locus_tag="VN496_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXF18350.1"
                     /translation="MLNIEDYALIGDCQSAALVGRDGSIDWLCWPRFDSAACFAALLG
                     TRDNGRWRIAPVDANAHTSRTYHDDTLILETTWENTDGAVSVIDFMPVRTRQPDLVRL
                     VVGRRGRLAMDMEFLLRFDYGAAVPWVTRLPGNAICAVAGPDKVVLCSPVPMEGRDFS
                     HTARFEVKEGERMPFVLTHALSHLNLPAKIDPLSALTDTETFWREWCARCSVKGQWLG
                     AVRRSLITLKALTYAPTGGIVAAPTTSLPEKLGGVRNWDYRYCWLRDATLTLLALMNG
                     GYRDEAESWTNWLLRAVAGSPAQTQIMYGLAGERRLNEQSLPWLPGFAGSKPVRIGNA
                     ACNQRQLDVYGEVLDVMHQSRKAGLAVEAAWSVQRAMLEHLETVWHEPDSGLWEVRGP
                     PRHFTYSKIMSWVAFDRGVKAVEEFQLSGPLDRWRRLRNEIHADVCRNGYVEARGAFV
                     QSYGSEHLDASLLLIAGTGFLPWDDPRVRSTVAAIQRELTVDGLVMRYHTHSSVDALP
                     PGEGVFLACSFWLADALYMQGQREEAHALFERLLALRSDLGLLAEEYDPVACRQLGNF
                     PQAFSHVALINTAINLSQLNKPMEQRAEDKAA"
     gene            6148..6399
                     /locus_tag="VN496_15190"
     CDS             6148..6399
                     /locus_tag="VN496_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18351.1"
                     /translation="MTQAKVTFKDIYTRKVGGERYEYEVTFTAGERVEWGARVYQNGV
                     LKGTPGGVETGNRLTGEALRQNIISLVEVAIEGMQGIKE"
     gene            complement(6436..6708)
                     /locus_tag="VN496_15195"
     CDS             complement(6436..6708)
                     /locus_tag="VN496_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18352.1"
                     /translation="MRRQIAASKAALWKSVGALLQVGALVALLGLLPYTLFSAYGLYQ
                     AVAAFGGDSIGSLVYQIARFSGCIVLIYALWRLRQAGLRLRNSQLS"
     gene            6917..8443
                     /locus_tag="VN496_15200"
     CDS             6917..8443
                     /locus_tag="VN496_15200"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXF18353.1"
                     /translation="MSESAATPIVTIRNLSKSFSGVSALDDCQFDLLSGEVHALMGEN
                     GAGKSTLMKILSGVYRRDSGEILLDGRAVDIASPRAAQAHGISIIHQELTLMNHLSAA
                     QNIFIGREPRGRLGVFLDETALNNQARRIFEQMHLQLDPRVRVGTLSVAKQQMVEIAK
                     ALSFDSRVLIMDEPTAALNNAEVADLFRIIRQLKSRGVAIAYISHKMDELQQIADRVT
                     VMRDGRYIGTVPMATTRVDRIISMMVGRELVDAPNDIPDTSRQDMALEVRHLNRGAAI
                     RDVSFSLRKGEILGFAGLMGAGRTEVARAVFGADPIDSGEIRVYGKKVSIKSPKDAVV
                     HRVGYLSEDRKHFGLATGLDVATNIVLSSMKRFLKLGGLVDATGIRAAAQAYVKQLQI
                     KTPSVEQQVRLLSGGNQQKIVIAKWLLRDCDILFFDEPTRGIDVGAKSEIYKLLNALA
                     EQGKAIVMISSELPEVLRMSHRIVVMCEGRITGEVPGRGATQEMIMQFATQRDAAKAA
                     "
     gene            8456..9484
                     /locus_tag="VN496_15205"
     CDS             8456..9484
                     /locus_tag="VN496_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF18354.1"
                     /translation="MSSTQPVDLAVQRPGVIGAVKSIFFDPATRQKLLAFASLLVLMV
                     FFSVASPNFLQADNMVSILQSTAVNGVLAIACTFVIITGGIDLSVGTLMTFCAVMAGV
                     VLTFWGLPIYLGIFAAIFFGALCGFTSGVLIAKLKIPPFIATLGMMMLLKGLSLVISG
                     TKPIYFNDTPEFSAISQDSLIGYFVSSAPIPNAVLILFLVAVAASIVLNKTLLGRYTF
                     ALGSNEEAVRLSGVNVAFWKIVVYSLNGAICGIAGLLIASRLNSAQPALGQGYELDAI
                     AAVVIGGTSLSGGTGTILGTIIGAFIMSVLINGLRIMSVAQEWQTVVTGVIIILAVYT
                     DILRRGKQ"
     gene            9552..10493
                     /locus_tag="VN496_15210"
     CDS             9552..10493
                     /locus_tag="VN496_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18355.1"
                     /translation="MATIRALTIALGLSVTFGLSANALAQETYIPLISKGFQHQFWQA
                     VKQGAEQAGKDLKVKVSFEGPETEAMVDKQIDMLSAALAKKPKAIGFAALDSKAAIPL
                     LKKAQAANIPVVAFDSGVDSDIPVTTATTDNKAAAALAADKMAELIGKSGEVAVVAHD
                     QTSRTGIDRVEGFTQRMKSTYPNIKVVSVQYGGGDHLKSTEITKSILQASPNLKGMFG
                     ANEGSAIGVLNGAKEMKRKIVVIGYDSGKLQKDAIRNGTMAGAITQNPVGIGYKTVEA
                     AVKALKGEKLPKIIDTGFYWYDKSNISDPKIAAVLYD"
BASE COUNT         2269 a         3089 c         3118 g         2112 t
ORIGIN      
        1 ccggtgacga ccagcaggcg aacacccacg ttggcggcgt caatgcgacc acacctgctg
       61 gcagtttgaa caatggccaa atcaatggtg catggaacac ggcaaccaat accgatgaaa
      121 gctgcctgtt ctggtcgcat atccggatgg cgaacctggg ccctggtgcc acaacgccgg
      181 cttgcaatgc ggcgggctac tacccggtaa atgcgtccgg cggtcagatt ggcgtgcaaa
      241 gctccaatgg gttcacgacc attacattgc caacgccaat gaccggtacc tatgttattt
      301 gttcagccaa cattcccgga gaatatgtca tccagctcga caccttgctg gacgatggcg
      361 atccgcaaac cggttccatg cgcgcaatcg accaatctgc gcttgccgcc gccaccgctg
      421 caacgatcgc gggcgtgcag gctaaccttg gcggcaacta cacggtttgc ctcagcgtct
      481 agcagaaata ttgttcgcgt tacacaggtc ataccggctt gcctgaaacg tggctggaaa
      541 aaaatgtgat ggcatgcccg gcaggttcac ggactgtgaa gacctcgtgg ccaatcgctg
      601 ggcggctctc gattggcgag ggcgcaaggc cctgagacgc aaatcgctgg tgggtcgcat
      661 gcaggtcatc aaccatgaga tcgaactggg cgttacccgg aacgatggag ttcttcagca
      721 tcaggattaa gtgcgtcccg ccgcgtagtt cgtaaacgcc gacctccggt ccttcgaata
      781 tggggcgcat gccgatcgtt ttcatgaagc ttgctgactc ctgcatgcga ttcgtctcca
      841 gaacgacgtg ggccacacct atgggaggac gatgatcgtg gtcgctcatt tatttgtggc
      901 cgacaaattg caacgatgaa gcgcgccggg cccttagctt gtccgggtgc atgtcaggtt
      961 tccctgcgtt gtagcacgtg ctcgatgcgg cgatcgggga tcagccagat cagcgcaacc
     1021 aacacataca ttgcctgagc cacccattgc acccagaaag tcgaagcaat cgcgataacg
     1081 tagaagagcg gcgaaagctt ccctttccag tcttttccga cggctgtttt caggacggag
     1141 gccgaacctt cgctggcaat gagtgcttgt tccaggatcc aatacgcgat cgcggccatg
     1201 agcagcactg cgccgtacag ggcggagggc atcggggcaa agtggttctc gcccatccat
     1261 cccgtcgcaa atggaaacag ggacagccaa aacagcagat gaaggtttgc ccagagcacc
     1321 gtacccgaca cccgcccggt cgcatgcagc atgtggtggt ggttgttcca gtagatcccg
     1381 acatatacga agctcagcac ataactcatg aaaaccggca gcagcggcag caaggactcg
     1441 agagattcgc catgtggcac cttcatttcg agcaccatga tggtgatgat gatcgcgatc
     1501 accccatcac tgaacgcttc caggcggcct ttacccatga tctcgttgcc tggcgttgcc
     1561 gtttacgagc gtttacccaa aaacacgtgg gccgctcgtt cacccgcgac gtaccgttga
     1621 cattcatagc cgagcgcgtg catatcaatg tcgttccata ggtgctcgcc ggaacccagc
     1681 aaaacaggcc ggatggcaaa atgcaattcg tcgatcaggc cggcgcgaag atattcgcga
     1741 atagtggtta cgccgccgcc aaggcggatg tccagtccct tcgctgccgc ggtcgcctgc
     1801 tcgagagcag cgcgaatgcc ttgcgtaaca aagcgaaact cagtgcctcc ggccatcctg
     1861 agcggcggcc gcgggtgatg tgtcaacaca aataccggtg tgtgatacgg cggctcttct
     1921 ccccaccagc ccttccagtt ttcatccggc cacgggccgc ggaccgggcc gaacatgttc
     1981 cgccccagga tccaggcgcc gatattggcg aagccctgtt ctgccattgc gttgtcggtt
     2041 ccggtctcgc catcgccatc gccgtacatt cggcgaaata cccgtgtggg gaaaaaccac
     2101 tccatcaact cggtgcctct gacgccgagc ggattctgca ggtcctggtt cggaccagca
     2161 ccgtacccgt caatcgaaac cgaaaaactt tggacgcgga gttttgacat gtctggactc
     2221 gagagatgca atatatctgt atctggttat gaatatgcca agccggctct cattacgtcc
     2281 attacctggc agactggctg gacattagcg ttcggtcacc gagcttgcag atgggttgct
     2341 ttagcgtgcc gccggtggtt ttgactgtcg gtgaagcact gccgccaatc cgaggggaaa
     2401 ctgcgattgc tgtgtttgtg atgggccgcg tgaaggagcg cacctcttca cgacttccgg
     2461 ccactggcaa tcagtgccgg catgggcagc gcaatgccgc caccagcagc cccgtaccga
     2521 tttgcttcac gctctacttc ggcccgaatg tttgcgaggt tcttcggtga ctgcgcgcgc
     2581 agcagagctc ccgtgcgcac ggtgctggcg ctgatgccgt cgaagaaagc ggtggcagaa
     2641 gcgaagtgcc aggtctgcgg cacctccgtg atagtgacgg atttgaaacc ggcgcccgca
     2701 atagcctgac tgcattgtgc ggcgtcgctg aagcggaaga acggaggccc cggcggcagc
     2761 ccaacgttca tgctgccatg cttctcgaca gcgcgcatga tgataccgaa tccgacggcc
     2821 ttgtcgggtc ctgcccacac cgaaaggcca aagcgcccac cgctgcgcag gacacggcag
     2881 gcttcgcgca aggcgcgatc cggatctgga aaatgcagga tgccgaatcc aatcacgacg
     2941 acatcgaagc tggcatcttg aaacggcaga tcgcctgcat cgccttcgcg aaaatgaatg
     3001 ccgggatgaa gcgcggcggc ttcgctgatc atggcggaag aaaaatcgag gccggtgatt
     3061 tccgcaccgc gctgcgcagc ggcagcggcg atgtaacccg ggccggtcgc cacatcgagc
     3121 acgctgtccc cggaatgcac gttcaccgca ttgagcagcg gctcgatgga ctgcgcggtc
     3181 agtctgccga aatggtcctc atagagtttt accacgcgct gccaggcctg gtgttcgaaa
     3241 tcacggaacg tcgcgggtgg gaaatcggct ttcatgattg tgcccgtccc gagtgttgcg
     3301 ctaggaagtg cgctgccgcg catcggctgc catcatgaaa ttcctgcgca gcgccagcgg
     3361 gtcagaaata tttttgaagg gcgcactatg tatcccggtg ccggagataa ccacggagcc
     3421 aaaatcgaac aggcgtccga aaatgctctg attgacctga atgctttcta ttttctccag
     3481 gaacatttcc gtagtgcggc gctcgatcag tccggtcttc gcgattacgc gcttgttggt
     3541 gatggcgagc tcggtagttg cataaagaat ccaggcccac agccacagca gcaatccgat
     3601 gccgaacagc ggaacgagca gcgcgccaaa cacgatctgc ggccacagag accatttgct
     3661 caggcgggcg cgataaagca cccgctcgtc attgatcaat acctggtcga tataggaagc
     3721 catggccgtt tgcgcttaaa gccgcatgat gccggtgaaa cccgtgcagg ggcaacccga
     3781 aagtgccctg gctttagttg ttcggcttgc tgctcttatt ttcggatttc gactggccct
     3841 tttcagcggg cgccggtgta ttgctttcgg tcttggaacc ggatgcggct ttgcgcgctg
     3901 cgtcgacttc gggacgatcg gtcttggtgg tttcaggagt gacccgggcg ccggtgtggc
     3961 cacccggatc agggcccata gccaaccagt cattggtagg cgttgcgtcc ggcgtacccg
     4021 ctcttgccaa tgcacaaatc atgaaaagca cgaatggcag cagcttcaac atgaaaacct
     4081 cctacgcgaa tgtgcttggt ccggcgctcg cgccgcatat ggcgcgctct accgatagaa
     4141 caatcgcagg aagtaaagtt ctccacaggc tggattaagc ctttgcagtc aggcggcctt
     4201 gtcttcggcg cgctgctcca tgggcttgtt aagctggctc agattgatgg cggtgttgat
     4261 gagggcgaca tgggaaaacg cctggggaaa attgccaagc tgacggcatg ccaccggatc
     4321 atattcctcc gcaagcaggc cgaggtcgct gcgcaatgcc aacagccgtt caaatagcgc
     4381 gtgcgcctct tcacgctgtc cctgcatgta caaggcatcg gccagccaaa agctgcatgc
     4441 caggaacacg ccctccccgg gcggcaaagc atcgaccgaa ctgtgtgtgt gataacgcat
     4501 caccaatcca tcgaccgtga gttcgcgctg gattgcagcg acggtggatc tcacacgcgg
     4561 atcgtcccac ggcaggaagc ccgtcccggc aatcagcagc aggctggcat ccagatgttc
     4621 cgatccgtag ctttgcacga aggcgccccg cgcttcaaca tacccgttgc gacaaacgtc
     4681 ggcgtgaatt tcgttgcgca gccgacgcca acgatcaagg ggcccggaca actggaattc
     4741 ctctaccgct tttacgcctc ggtcaaacgc cacccaggac atgattttgg aatacgtgaa
     4801 gtggcgcggc ggcccgcgta cttcccatag tccggagtcg ggttcgtgcc agactgtctc
     4861 gagatgttcc agcatcgcgc gttgcacgct ccacgctgct tccacggcca gcccggcttt
     4921 gcgcgattga tgcatgacgt cgagcacttc accgtacaca tcaagttgcc gttggttgca
     4981 cgcggcattg ccaatccgca ccggtttgga tccggcaaat ccgggcagcc agggcaagga
     5041 ttgttcgttc aggcgtcgct cgccggccag gccgtacatg atctgcgtct gtgccgggct
     5101 gccagcaacc gcgcgcaaca gccagttggt ccagctttca gcttcgtccc gataaccgcc
     5161 gttcatgagc gcgagcagcg tcagggtggc atcgcgcagc cagcaatagc gatagtccca
     5221 gttgcgtacc ccgccgagtt tttccggcag cgatgtcgtt ggggccgcga cgatgccacc
     5281 ggtcggcgcg tacgtgaggg ccttgagggt tatcagcgac cggcgaacgg caccaagcca
     5341 ttggcccttc accgagcagc gtgcacacca ctcccgccag aacgtttcgg tatcggtcaa
     5401 ggcactcagt gggtcgattt tcgccggtag attgagatga gacaacgcgt gcgtcagcac
     5461 aaaaggcatg cgctcgcctt ctttgacttc gaagcgggca gtgtgcgaaa aatcgcggcc
     5521 ctccattggt accggagagc acagcacgac cttgtctgga ccggccacgg cgcagatcgc
     5581 attgcccggt aggcgagtaa cccagggaac tgccgcgccg taatcgaagc gcagcagaaa
     5641 ctccatgtcc atcgccagac ggccccggcg tccaaccacc aggcgcacca gatccggctg
     5701 tcgcgtgcgc accggcatga aatcaatcac gcttaccgca ccatctgtgt tttcccacgt
     5761 ggtctcgaga atgagcgtgt catcgtggta agtgcggctg gtatgcgcat ttgcatccac
     5821 gggtgcgatg cgccagcggc cgttatcgcg cgtacccagc aacgcggcga agcaggcggc
     5881 agagtcaaag cggggccagc agagccagtc gatcgaaccg tcccgcccga ccagcgcggc
     5941 gctctggcag tcgccaatca gcgcatagtc ctcgatgttc agcatggaac cccaggcaca
     6001 gttgactgca gttcggcgta cagtaacaac acgctccgca taccatgaag gaccgcgcgg
     6061 ccatgcgata agttctatga cgtgccgtgt gacgcagttc agcgggctcg ctgcagcatg
     6121 tcggggaaac tttttttggg atatcgaatg acacaagcga aggtcacatt caaggatatt
     6181 tacacccgca aggtaggggg cgagcgatac gagtacgagg ttacctttac ggccggggag
     6241 cgggttgaat ggggcgcccg cgtttaccag aacggtgttc tgaagggcac gcccggcggt
     6301 gttgaaaccg gcaatcggct aaccggtgaa gccttgcgtc agaacatcat ctcgctggtg
     6361 gaagtcgcca tcgaaggcat gcagggtatc aaggaataaa ttcccgcgca caacgcccgt
     6421 cagccgcatc gacggctagg acaattgact gttgcgcagc cgcaatccgg cctggcgcag
     6481 tcgccacagc gcatagatca atacaatgca tccgctgaaa cgtgcaattt gataaaccag
     6541 agagccgatt gagtcgccgc caaaagccgc cactgcctgg tataacccgt atgcggagaa
     6601 cagggtgtag ggaagcaggc cgagcaaagc caccagagcg ccaacctgaa gcagcgctcc
     6661 gacggatttc cagagcgctg ctttcgatgc ggcaatttgt ctacgcatgc tcgccctgcc
     6721 aattcttcgg cgtttgatac cgcaaatgca cggccggttt ccggttgtgt cccatgctca
     6781 agagctgacc gatcggagac atgatcgtac aggagatgcc attattacgg ctggatagcg
     6841 gcacttcacc gaagttctgt tgcctgggct tttgggcaca ctgtagactt ggcgggatta
     6901 ccctcctggt gtaccgatga gcgaatccgc cgcaacgcca atcgtcacga ttcggaattt
     6961 gtcgaaatcg ttctccgggg tttccgcact ggacgactgt cagttcgact tgctctccgg
     7021 cgaagtgcat gcgctgatgg gtgaaaatgg agcgggcaag tcaaccttga tgaagatatt
     7081 gtccggggtg taccgcaggg attccggtga gatactcctc gacggtcgtg cagtagacat
     7141 agctagtcca cgcgcggcac aggcccatgg catcagcatc attcaccagg agctcacgct
     7201 gatgaaccac ctcagtgcgg cccagaatat tttcataggc cgtgaaccgc gcggacggct
     7261 cggtgtgttc ctggatgaga cagcactaaa caatcaggcg cggcgcatct ttgaacagat
     7321 gcacttgcag cttgatccgc gcgtgcgcgt cggaacgctg tcagtggcca agcagcagat
     7381 ggtcgaaata gcgaaggcgt tgtcctttga ttcgcgcgtg ctgatcatgg acgagcccac
     7441 tgcggcgctg aacaacgccg aggtcgccga tttgttccgc atcattcgcc aattaaagtc
     7501 ccgcggtgta gccatcgcct acatatcgca caaaatggat gagttgcagc agattgccga
     7561 ccgcgtgacc gtgatgcgcg acggccgcta tatcggcacg gtgcccatgg caaccacgcg
     7621 tgtcgaccgc atcatcagca tgatggtggg gagggaactg gtcgacgcgc ccaacgatat
     7681 tccagatacg tcgcgccagg acatggcact ggaggtacgg catttgaatc gtggtgccgc
     7741 tatccgcgac gtcagtttct cgctccgaaa gggcgaaatc ctgggcttcg caggactgat
     7801 gggcgcgggc cgcaccgaag tcgcccgcgc cgtattcggt gccgacccga tcgattcggg
     7861 tgaaatccgc gtttacggca aaaaagtttc catcaagtcg cccaaagatg cggtggtgca
     7921 ccgcgtgggt tatctttccg aagatcgcaa gcatttcggc ctggcaacgg ggctggatgt
     7981 agccaccaac atcgttctgt ccagcatgaa gcgttttttg aagctgggcg ggctggtgga
     8041 tgcgaccgga atccgtgcgg ccgcgcaagc gtacgtcaag caactgcaga tcaagacgcc
     8101 gtcggtcgag cagcaagtgc ggctgctctc gggcggaaac cagcagaaga ttgtcatcgc
     8161 gaagtggctg ctgcgggact gcgacatcct gttcttcgat gaacccactc gcgggatcga
     8221 cgtgggcgca aaaagcgaga tctataaatt actcaacgcg ctggcggagc aaggaaaggc
     8281 catcgtgatg atatcttccg agctgcccga agtactgcgc atgagccacc gtatcgtcgt
     8341 gatgtgcgaa ggccgcatta cgggcgaggt gcccggccgc ggcgcgacgc aggaaatgat
     8401 catgcagttc gccacgcagc gagatgccgc gaaagcggcc tgaacgagga cctccatgag
     8461 ctccacccaa ccagtcgatc ttgccgtaca acgccccggc gtgatcgggg ccgtcaaaag
     8521 catttttttc gatccggcga cgcgccaaaa attgctggcg tttgcgagcc tgctggtgct
     8581 gatggttttc ttcagtgtcg catcgcccaa ttttctgcaa gccgacaaca tggtcagcat
     8641 cctgcagtcg actgcggtca acggcgtgct tgccattgcg tgcacgtttg tcatcatcac
     8701 cggaggcatt gatttgtccg tcggcaccct gatgaccttc tgcgccgtca tggcgggcgt
     8761 ggtcctgaca ttctggggcc tgccgatcta cctgggcata tttgcagcga ttttctttgg
     8821 cgccttatgc ggtttcactt cgggcgtgct gatcgccaag ctgaagatcc cgcctttcat
     8881 tgccacgctt ggcatgatga tgctgctgaa gggactgtcg ctggtcatct ccggtaccaa
     8941 gccaatctac ttcaacgaca ctcccgaatt ctcggcgatt tcccaggact cacttatcgg
     9001 ttatttcgta tcctctgcgc cgattcccaa tgccgtgctg atcctctttt tggtagccgt
     9061 cgccgccagc atcgttctga acaaaacgct attgggccgc tacacctttg ctttgggcag
     9121 caatgaggaa gctgtgcgcc tgtccggagt caatgtcgca ttctggaaaa ttgtggtgta
     9181 ctcgctgaac ggcgcaatct gcggcattgc cgggctgctg atcgcctcgc gcctgaattc
     9241 cgcgcagccg gcgctcggcc agggctacga gctggatgca attgccgcgg tcgttatcgg
     9301 cggcacgtcg ttgtccggcg gcacgggaac catcctgggt accatcatcg gcgccttcat
     9361 catgagcgtg ctgatcaacg ggttgcgtat catgtcggtc gcccaggagt ggcagacggt
     9421 ggtaaccggc gtgattatca tcctggcggt ctacacggac atcttgcgca ggggcaagca
     9481 gtaagcgctg tacagcatgc aaacaaatcc gcaaccggac ggactccgga aaaatcagag
     9541 aggagaatta catggcaacg attagagcat tgacgatcgc gctcgggctg agcgtcactt
     9601 tcggcctgag tgcgaacgca ttggcgcagg aaacctatat tccgctgatc tcgaaaggct
     9661 tccagcacca gttctggcag gcagtgaagc agggcgccga gcaggccggc aaagacctga
     9721 aagtcaaagt cagtttcgaa ggcccggaaa ccgaagcgat ggtcgacaaa cagatcgata
     9781 tgctgtccgc tgccctggcc aagaagccga aggcgatcgg cttcgccgcg ctcgacagca
     9841 aggcggcgat tccgctgctg aagaaagcgc aagccgcaaa cattccagtc gttgcattcg
     9901 actcaggagt ggatagcgac ataccggtaa cgacggccac cacggacaac aaggctgctg
     9961 cggcgctcgc ggccgacaag atggctgaac tgattggcaa gtccggcgag gtcgccgtgg
    10021 tagcccacga tcagaccagc cgcacgggca ttgatcgcgt agagggcttt acgcaacgca
    10081 tgaagtcaac ctatcccaat atcaaagtgg tcagcgtgca gtacggcggg ggcgaccacc
    10141 tgaagtccac cgaaataacg aaatcgatcc tccaggcctc tcccaacctg aagggcatgt
    10201 tcggcgcgaa cgaaggttcc gccattggtg tgctcaacgg ggccaaggaa atgaaacgca
    10261 aaatcgtggt gattggctac gactcgggca agctgcagaa agatgcgatc cgcaatggca
    10321 ccatggctgg cgccatcacg caaaatccgg tcggaattgg ctacaagacg gtcgaagcgg
    10381 cggtaaaggc gctcaagggc gaaaaactgc cgaagatcat cgacaccggg ttctactggt
    10441 acgacaagtc gaatatctcc gatccgaaga tcgcggcggt tctatacgac tgatgacttt
    10501 ggtttagatg gcacacatcg ggcttgctgg cggctggccc gatgcgcacc aaccgccgca
    10561 tacggcgcgg ggactgtttt tcatgcgg
//
LOCUS       DATMZQ010000477         8688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4753244, whole genome shotgun sequence.
ACCESSION   DATMZQ010000477 DATMZQ010000000
VERSION     DATMZQ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4753244"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..404
                     /locus_tag="VN496_15215"
     CDS             210..404
                     /locus_tag="VN496_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18356.1"
                     /translation="MTPFADEVLRKSSTWKPNFGSLTPMFEPNIGIPTLSFVYSNCVE
                     LAFTREMIEPFGTLISPPAM"
     gene            complement(447..1643)
                     /locus_tag="VN496_15220"
     CDS             complement(447..1643)
                     /locus_tag="VN496_15220"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HXF18357.1"
                     /translation="MSPLHSHLKNALLGLFTIFFLLLLLTGCGSGVDSGGTGAAAASA
                     SGPITGFGSVIVNGVHFDESHATITDADGTPHHRDDLKLGMTTQIRGSALVVNANETR
                     SIADSIVFSSAILGPIESIDTQARKLTVLGQSVDVSSMTIFDDSLTGGLTALAVGDVI
                     LVYALFDGADSTYSATRIEPKSGVAAYELRGVANNLNTSLREFSIGKLRISYAGIDPK
                     KVPPSLANGRLVRLKLQTAMTTGVVNAMQVLDGVNQLGEHDEVRIEGLISSSGSNGQF
                     TVEGVPVDAAQAGVSGNAGLGVGMRVEVEGTVSSGVLVATKVHSKTQKEVEGEGFELD
                     GSIAALDPVNRTLVVRGISVDYSGTVEFRDGTIADMAVGKKVEIKGTLSSDRTGLSAV
                     RIKFKH"
     gene            complement(1645..2568)
                     /locus_tag="VN496_15225"
     CDS             complement(1645..2568)
                     /locus_tag="VN496_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HXF18358.1"
                     /translation="MATRTSTGKSKRPESGTPAAALLDHASLLQALKTLLQPLATLSV
                     ARGMSFAVVEEMLKKAFVDAARITHSNAAHRVVSRISTATGLNRREVSRLLKSTTRRA
                     PIRRSPATEVFTRWIAEPSLKNAQGEPLPLPRQGPSSSFEKLARSVNRDVHPRSLLEE
                     LLRLGLVRLEGETVHVVRDSFVPKQDVGRMLGFLGHNVGDHFRAAVANVLADSPSHLE
                     QAVFADELSQESLDHLRDVMRAQWKGLLEATVPLLHKLIAADRSASRDRGQRVRVGIY
                     MFSEPMAAPLTGGPDVKRRPRSQKASLKKGT"
     gene            2977..3441
                     /locus_tag="VN496_15230"
     CDS             2977..3441
                     /locus_tag="VN496_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18359.1"
                     /translation="MTPEHIHTVQSSWNKALPAGDGEARVLFESLLQGDTSLDGLFQL
                     DPPTWSANVVQMVDVVVAGLTRRDRSTELVRRVGSRNTACPAIEDHYDVIGTALLRAL
                     ARILGAAFTPRIEAAWTIVYAQLVVVMQKAAAAKRIRRISRMTSDVRILGGA"
     gene            3464..4048
                     /locus_tag="VN496_15235"
     CDS             3464..4048
                     /locus_tag="VN496_15235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXF18360.1"
                     /translation="MTSSATSLETGLDSGYKPATQFVRLKNDRLIALRVACFEDGSAI
                     QQFVRGLSLRSRRNRFFSAMRELPNELIEDLRSHTSGKLSLIGETLEGPKSRIVAMAQ
                     YAVYEPIVAELAVVVDDAWQRQGLGLDMLVRLAEHAARAGLSALTGFVLPDNGPMLKL
                     LSRWDCEIVSSGDPYMMRFVKQFDARQRNLVGAL"
     gene            4237..4644
                     /locus_tag="VN496_15240"
     CDS             4237..4644
                     /locus_tag="VN496_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18361.1"
                     /translation="MRPVAIWLVILMFAVVNGAFRETVLVPNLGKPVALVLSGLLLSL
                     IILTLTFVLAPWIRLDTRARCLFVGLLWVLLTLVFEFGFGLSQGRSLPDMLEAYTFKD
                     GNIWPLVLLVTFFAPLMAARLRGTPKDGVARNE"
     gene            4683..5051
                     /locus_tag="VN496_15245"
     CDS             4683..5051
                     /locus_tag="VN496_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HXF18362.1"
                     /translation="MIPHMILVAGPYRSGTNDDPKLIARNVAAMTDASLMLFRAGHLP
                     VMGEWFALPLIEHAGSSGIGDAVFNEIFHPISRRLIAKCDGCLRIGGASAGADEMVAL
                     ARQHGKAVYYSPEEIPGAAP"
     gene            complement(5107..5373)
                     /locus_tag="VN496_15250"
     CDS             complement(5107..5373)
                     /locus_tag="VN496_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18363.1"
                     /translation="MKLLRSLFTIAALILASSAWAYQVTGPVLEVSDSKIVVQKGKEK
                     WEIARTPDTKVTGDLKVGSKVTVEYMMTAKSVDVKTDKAKSKKK"
     gene            5557..5889
                     /locus_tag="VN496_15255"
     CDS             5557..5889
                     /locus_tag="VN496_15255"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXF18364.1"
                     /translation="MKGIENDVQTTLVDWAARTPKVRRVWVFGSRARGNHRPDSDIDI
                     AVELEPVADSEETLLVWLANSEKWRSQLQDRISMPVDLEWFDPNGGTGTIRAALDEAK
                     TLVYEAAI"
     gene            5999..6385
                     /locus_tag="VN496_15260"
     CDS             5999..6385
                     /locus_tag="VN496_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HXF18365.1"
                     /translation="MAIRIVRLGTERVPNEGLRIGTVRRPPRGVPKSEFSTGNWYDVW
                     FPNLAPSAETVNLALKAASAREWSAFIKRYRAEMAQPENSRVLDVLAALSQSANFSLG
                     CYCEDESRCHRSILRELLCEHGAKVL"
     gene            complement(6434..6973)
                     /locus_tag="VN496_15265"
     CDS             complement(6434..6973)
                     /locus_tag="VN496_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HXF18366.1"
                     /translation="MLVAVVHLFSLTLFSEVAMAACPDLLNHQFKNLDGKPIDLCSFS
                     GKVVLAVNTASYCGNTPQYKGLEALYQKYRDQGLIVVGFPANDFGAQEPGSSKEIKEF
                     CELTYGVKFPMVEKTSVVPGKANPVFAQLEKMTGDAPEWNFHKYLIARDGKRAFSFSA
                     RTQPESKEVVNRIEELLAE"
     gene            7294..8481
                     /locus_tag="VN496_15270"
     CDS             7294..8481
                     /locus_tag="VN496_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXF18367.1"
                     /translation="MGPLSDIKVLELGTLIAGPFCSRVLAEFGAQVIKIEAPDGGDPL
                     RQWRKLHHGTSLWWYLQARNKKSVTVNMRAPEGQQIVRELASETDIVIENFRPGTLEK
                     WGLGWEELSKRNPGLVLVRLSGYGQTGPYRERVGFGAIGEAMGGLRYVTGYPDRPPVR
                     VGISLGDAIAALYGVIGGLMALRHREVNGGKGQMVDVALYESVFSLMESLLPEFDMTG
                     FVRERSGASLPGIVPSNTYTTRDGKYVVVGANGDSIFKRMMLAIGRADLAEDPSLARN
                     DGRVVRTGELDKVIADWCAAHDLNRVLEVLDHAQVPAGKIYDIADIVSDLQYRARGMI
                     EQLRLPDGTQMKVPGVVPKLNDTPGGTRWPGPELGEHTHETLAGLGYDDGRIAALKSR
                     GII"
BASE COUNT         1816 a         2470 c         2489 g         1913 t
ORIGIN      
        1 ttctgagcgc cgcccgctat tttccaaggc cgtgtagcga cacttcttgc ttcgggccgc
       61 tagttttaga accgtctcaa catgcggcat agttcctggt tggagctggg atagcttctg
      121 aaaggcgctg ttccaattac acgagcgctt cgccattgat gcagctatcg gaagccaacc
      181 gattatttcg ccaaaccaag gaacggcctg tgaccccttt cgcggacgaa gtgcttcgca
      241 aatcgtcaac ttggaaaccc aacttcggtt cgctgacgcc aatgttcgag cccaatatcg
      301 gcattcccac tttgtcgttt gtttattcga actgtgtcga actggccttc acgcgagaga
      361 tgattgagcc attcggtact cttatttctc cacccgcaat gtagccgccg taccattaca
      421 agatatagag gcttatacat ggcgcttcag tgcttgaatt taattcggac agcacttagt
      481 ccggttctgt ccgacgacag tgtgcccttg atttccactt tctttcccac tgccatgtca
      541 gcgatggtgc catcgcgaaa ctccacagtc cccgagtagt ctaccgaaat gccacgtacc
      601 actagtgtcc tgttgacagg gtccagtgcg gcgatggagc catccagctc gaaaccttcg
      661 ccctcgactt ccttctgcgt tttactgtgt accttggtgg caaccaggac gccactgctt
      721 acggtgcctt cgacctcgac tcgcatcccc acgcccagac cggcatttcc tgatacgccg
      781 gcctgagcag catcgactgg aacgccttcg acagtgaact gcccgtttga tccagaactg
      841 ctaatgaggc cctcgattcg cacttcatca tgttcgccaa gctggttaac accatccagc
      901 acctgcattg cgttgacgac gcccgttgtc atggccgttt gtagtttcag gcggactagt
      961 cgtccatttg caagcgaggg tggcaccttc ttggggtcta ttccggcgta agagattctc
     1021 agcttgccga tagaaaactc cctgagcgac gtattgagat tgttggccac gccgcgcagt
     1081 tcgtaagccg ccacgcctga cttgggctca atgcgcgtgg cgctgtaagt gctgtccgca
     1141 ccatcgaaca aggcatagac cagaatcaca tcgccgaccg caagggccgt cagcccgccc
     1201 gtcaagctgt catcaaagat tgtcatcgaa cttacatcca ctgactgccc aagcaccgtt
     1261 agtttgcggg cctgcgtatc gatgctctct atcggtccta ggatcgcgct gctgaaaaca
     1321 atgctatcgg caatgctgcg cgtctcgttt gcgttcacga caagtgccga gccgcggatc
     1381 tgcgttgtca ttcccagctt gagatcgtcc cggtggtgcg gtgtgccgtc agcatccgtg
     1441 atagttgcgt ggctttcatc gaaatgtact ccgttgacaa tcaccgatcc gaatccggtg
     1501 attggaccac tggccgaggc cgcagccgcg ccggtcccgc cactgtcaac gccgcttccg
     1561 cagcctgtga gcaacagaag cagaaaaaat atcgtgaaca acccaagcag ggcgtttttc
     1621 agatgcgaat gcaaaggtga catgtcatgt accttttttg aggctggctt tttgagaccg
     1681 cggacgtctc tttacgtcgg gacccccagt aagtggagca gccatgggtt cggagaacat
     1741 gtaaatgcca acgcgcaccc gttggccccg atcgcggctg gcagagcgat cggcggcgat
     1801 gagcttatga agcagaggga ctgtcgcttc cagcaagccc ttccactggg cacgcatgac
     1861 gtcgcgtaga tgatcgagcg actcctggga caactcgtcg gcgaatacgg cctgctccag
     1921 atgcgaggga gaatcggcaa gcacgttggc gaccgcagcc cgaaaatgat caccaacatt
     1981 gtggccaagg aatccaagca tccgtccgac gtcctgcttc ggcacaaaac tgtcccggac
     2041 cacatggacc gtctcacctt ccagccgcac caagccaagg cgtaggagtt cctcaagcag
     2101 actgcgtggg tggacatcgc gattcaccga tcgtgccagc ttttcgaaac tgcttgacgg
     2161 cccctgccgc ggcaatggca gcggctctcc ttgggcattt tttaatgacg gctcggcgat
     2221 ccagcgggta aacacttcag tagcgggcga gcgacgaatc ggtgccctgc gcgtagttga
     2281 cttcaacagg cgcgacacct cccggcggtt caggccggtc gccgtgctga tgcgactcac
     2341 cacacggtgc gcggcattgg aatgcgtgat gcgagccgca tcgacaaatg ctttctttag
     2401 catttcctct acgactgcaa atgacatccc gcgtgcaacg gaaagcgtag ccaagggctg
     2461 caacagtgtt ttcagcgcct gtagtagcga agcgtggtca agcaacgccg ctgccggcgt
     2521 gcccgactct ggtcgtttgc ttttaccggt ggatgttcgc gttgccattc gcaatcgttg
     2581 aatcgcataa cctggtcaat gtgccaaatg cccatttcaa ttgctgagga gagccgctca
     2641 aaatataggt ccattcagac catccgtgtc cgaagccata gcccgattgg ctacgttcct
     2701 gagccataca acgttcacct taagacccgt gagcgtccgc ttgcggtcgt aagcggaagt
     2761 gtcagcagtt ggcccgattg ccgactcgat ggcttgcgac ccaaagccga tgtttacgcg
     2821 cagcaggggt tctttttttg gttcggtgcc tttcctacgc agctcgcgcg aaacaaacga
     2881 aacaaaattg gtgggttcgt gaaattctcg taatgcgtct gctcaataca gtgcggatac
     2941 atcccatgga atgggaccac accaaggagc ggcgccatga caccagaaca catccacaca
     3001 gtgcagtcca gttggaacaa agcgctgccg gctggcgacg gagaagcaag ggtactgttc
     3061 gaaagcctgc tccagggcga tacctcgctt gacggattgt ttcagcttga cccgccgaca
     3121 tggtctgcga atgtcgtgca aatggttgac gtcgtggtcg cgggactgac ccgcagagat
     3181 agaagcacgg aactcgtgcg cagagtcgga agccgcaaca ccgcctgtcc ggcgatagag
     3241 gatcactatg atgtgatagg taccgcgctg ctgcgggcac tggccagaat cctcggcgca
     3301 gcatttacac cacggatcga agcggcctgg acaatcgttt atgcacagct tgtcgtcgtt
     3361 atgcaaaagg cggccgctgc gaaacggatc aggcgcattt cgaggatgac gtccgacgtt
     3421 cgcattctgg gtggcgcctg aatccccggc gtgatgcaaa gccatgacat catcggctac
     3481 ctcgctggaa actggcctcg attccggata caaaccggct acccagttcg tgcgcttgaa
     3541 gaacgatcgc ctgatcgcat tgcgtgttgc ctgcttcgaa gacgggtcgg cgattcagca
     3601 gttcgttcgg ggcctttcat tgcggtctcg ccgaaatcgc tttttctccg cgatgcggga
     3661 acttccgaat gagctgatcg aagacctgcg atcccatacg tcgggcaaac tgtcgctcat
     3721 cggtgaaacc cttgaaggcc cgaaatcgcg catcgtggcg atggctcaat atgcagttta
     3781 cgaaccgatc gtggccgaat tggcagtggt agttgacgac gcatggcagc ggcaggggct
     3841 gggcctcgac atgctggttc ggttagccga gcatgcagcc cgcgcggggc tttccgcgtt
     3901 aaccggcttt gtgctgccgg ataacggccc gatgctgaag ttgctatctc gctgggattg
     3961 cgaaatcgta tccagcggcg atccgtacat gatgcgtttc gtgaagcagt tcgatgcccg
     4021 ccaacggaat ttagtgggcg ccttataagc ggagtagcac atcgacgcat gcgacaacca
     4081 agccggatcc ggaccaacgt tcccgtcagg gatagaatga gtacggaatc gatgccgctg
     4141 cgtcaatgcg acaccgtcaa cacaatcgac acaaagaact atcccacgca gcaatagcga
     4201 caagatggcc ggctggcccg tcattccacg attaacatga gacctgtcgc aatctggctt
     4261 gtgatcctaa tgttcgccgt tgtgaacggt gcgtttcgcg agactgtctt ggttccaaac
     4321 ctgggcaagc cggtcgccct tgtgctgagc ggtctgctgc tgtcgttaat cattctgacc
     4381 ctgacgttcg tgctcgcgcc ctggatcagg ctggacacgc gagcacgatg cctgttcgtc
     4441 gggcttcttt gggtacttct gacgctcgtt ttcgaattcg gttttggtct aagccaaggc
     4501 cgttccctgc cagacatgct ggaagcctat accttcaaag atggcaacat ctggccgctg
     4561 gtgctgttag tcacgttttt cgccccgttg atggcggccc gcctgcgcgg tacgccgaaa
     4621 gacggcgttg cacggaatga ataagccaaa gcctcaatga ggcgttacag gaggttccac
     4681 aaatgattcc gcatatgatc ctggtcgccg ggccttatcg ctccggaacg aacgatgatc
     4741 ccaagctcat tgcgcgtaac gtggcggcca tgacagacgc ttcgctcatg cttttccgtg
     4801 ccggtcatct gccagtcatg ggcgaatggt ttgcgcttcc gctcattgaa catgccggct
     4861 cgtctggaat aggcgatgca gtgttcaacg aaattttcca tccaatctcg cgtcgactga
     4921 tcgccaaatg cgatggttgc ctgcgcattg gcggggcctc cgccggcgca gatgaaatgg
     4981 tcgcgctcgc aaggcaacac ggaaaagctg tgtactactc gcccgaagaa atacccggcg
     5041 cggcaccctg acggattccg aatccatcct gtctggattc gcttccggcc caactcgcgt
     5101 tcaaggctac ttcttcttcg atttcgcctt gtccgttttc acatccacgg attttgccgt
     5161 catcatgtac tcgacggtga ccttggagcc gactttcagg tcgccggtga ctttggtgtc
     5221 cggcgtgcgg gcaatctccc acttctcctt gcctttctgc acgacgatct tgctgtccga
     5281 aacttcgagc acgggtccgg tgacctggta ggcccaggca gaactggcga gtatcagcgc
     5341 ggcaatggtg aaaagactac gtagtaattt catcggaata ctccttgagg gatggtgacg
     5401 gcattgaaca ggcatttttg cgctgtctag ggtagcgctg tgcgccagcg tttgccaagt
     5461 atgcggctca tgaacagtta tgcgtccggg ctgtctaccc gtttctgggc gcaagtcccg
     5521 caggggcgaa acgctaatct ttaacggtaa atttacatga aaggcattga aaacgacgtg
     5581 caaacgaccc tcgtggattg ggcagcgagg acccccaagg ttagacgagt gtgggtgttc
     5641 ggcagccgcg cgcgcgggaa tcatcgtccg gacagcgaca tcgatatcgc cgtcgagctg
     5701 gaacccgttg cggacagcga agagacactg ctcgtgtggc ttgcgaattc ggaaaaatgg
     5761 cgctcccagc ttcaggacag gatcagcatg cccgtggacc tggaatggtt tgatccgaac
     5821 ggaggcaccg ggaccattcg tgcggcgctg gatgaggcaa agacgctggt ttatgaggct
     5881 gccatttgat gcggccgccg gtaaaacgat gttgccgcat actgtgaaat gatcgcgctt
     5941 taaatttcag ccgctcaaca gcgaccggcg acttgacagt ctcaagcgag gaaccgattt
     6001 ggccattcgc atagtgcgcc ttggaactga acgcgtgcca aacgaggggt tacgcatcgg
     6061 caccgtccgg cggccgccgc gcggcgttcc gaaatccgaa ttttcaacag gcaactggta
     6121 tgacgtctgg tttccgaatc tggcgccctc ggcggaaacg gtgaacctgg cgctcaaggc
     6181 tgcttcagcg cgcgagtgga gcgctttcat aaagcgctat cgagcggaga tggcgcaacc
     6241 ggagaacagc cgggtcctgg atgtgctggc ggccttgtct cagagcgcca atttttcgct
     6301 aggctgctat tgcgaagacg aatcgcgctg ccatcgctcc attctgcgcg agctgctgtg
     6361 cgagcacggt gccaaagtcc tgtagcacat gaatggcgcg tcccgctcac gcttcgaccc
     6421 ataaccagga gcgctattcc gccaacagct cttcgatacg gttgacgact tccttgcttt
     6481 ccggttgcgt gcgcgcggaa aaactgaacg cgcgcttgcc atcgcgcgcg atcagatatt
     6541 tgtggaaatt ccactccggg gcgtcgcccg tcattttttc cagttgcgcg aacaccggat
     6601 tggctttacc gggcaccacg ctggtttttt cgaccatggg aaatttgacc ccgtaggtca
     6661 gctcacaaaa ctccttgatc tccttgctgc tgcccggctc ctgtgcgcca aaatcattgg
     6721 ccggaaagcc gaccacgatc aatccctggt cacggtactt ctgatagagc gcttccagac
     6781 ccttgtattg cggtgtattg ccgcagtagc tcgcggtgtt caccgccaga acgaccttgc
     6841 ccgaaaacga gcacagatcg attggttttc catccagatt tttaaattga tgattgagca
     6901 gatcgggaca cgcagccatg gcaacctcgc tgaacagagt cagggaaaat aaatggacca
     6961 cagcaaccag catgcgtttc attccaattc ccctgtattc ggtatttgca gataatacgc
     7021 gtcttcgatg gcccaccatt ttgaattacg gaagttatgg tgaagaccgg ttatggtcga
     7081 tagaccggtt atggtcgaca gaccggtatc cctttaggga atcctttacg gaatcctgcg
     7141 aagcagggca cggcagttat gggcggtagc ccggtatcct gcgaagcagg gcacggtagt
     7201 cacggcagta acggcagtca cggtagtcac ggcagtcacg gcagtcacgg tagtcacggc
     7261 atttacggtc ttcacgcatt cacggtattt cccatgggtc ctctgtccga tataaaagtc
     7321 cttgaactcg gcacgctgat cgcagggccg ttctgttcgc gcgttcttgc tgaatttggc
     7381 gcgcaggtca tcaagataga ggcgccggat ggaggagacc cgcttcgcca gtggcgcaag
     7441 ctgcaccatg gcacttcgct atggtggtat ttgcaagccc gcaacaaaaa atcggtcacc
     7501 gtgaatatgc gcgcgcccga aggccagcag atcgtgcgcg agctggcatc cgagacggac
     7561 atagtcattg aaaatttccg ccctggcacg ctcgagaaat gggggctggg atgggaagag
     7621 ctgtcgaagc gcaatcccgg cctggtgctg gtgcggcttt cgggctatgg gcaaaccggg
     7681 ccgtatcgcg aacgagttgg attcggggcg atcggcgaag cgatgggagg attgcgttat
     7741 gtaaccgggt atccggatcg gcccccggtc cgcgtgggca tcagtctcgg cgatgcaatc
     7801 gctgccctgt acggagtcat cggcggcctg atggcgttgc gtcatcgcga agtcaacggc
     7861 ggcaaagggc agatggtcga cgtggcgctg tacgagtcgg tgtttagctt gatggaaagc
     7921 ttgctgcccg aattcgacat gaccggcttc gtgcgcgagc gcagcggcgc gtcgctgcca
     7981 ggcattgtcc cctcgaatac gtacacaacg cgcgacggca aatacgtggt ggttggcgcc
     8041 aacggtgatt cgatcttcaa acgcatgatg cttgcgatcg gccgggccga tctggccgag
     8101 gatccctcgc tcgcgcgcaa cgatggcagg gtggttcgca ccggagaact ggacaaggtg
     8161 atcgcggact ggtgcgcggc gcacgacctg aatcgggtac tcgaagtgct ggaccacgcg
     8221 caggtgccgg cgggaaagat atacgacatc gccgatatcg tgagtgatct gcaatatcgc
     8281 gcgcgcggca tgatcgagca actgcggttg ccagatggta cgcaaatgaa agtgccgggc
     8341 gtggtgccga agttgaacga tacgcccggc ggcacacgat ggccgggacc agaactcggc
     8401 gagcatacgc acgaaacgtt ggctggactg ggctatgacg acggccgcat cgctgcgctc
     8461 aagtcacgcg gcataatctg attttcccag cgacatcgtt ccgccagctg cattgtttca
     8521 gccgcagcgc ggcgtgtatt ttatacaaga cgccttgaaa atctggcagc ggcgcgtgcg
     8581 attcgcaccg gcaagccgcg cagatgtcct ccagcccgtg attttcctgg gcgcgccttt
     8641 tgcatgaggg ctgtccactg cggcatacat gaccctgcca acgagcag
//
LOCUS       DATMZQ010000478         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4404687, whole genome shotgun sequence.
ACCESSION   DATMZQ010000478 DATMZQ010000000
VERSION     DATMZQ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4404687"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="VN496_15275"
     CDS             <1..2115
                     /locus_tag="VN496_15275"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXF18368.1"
                     /translation="DVEPRRIPSTGEVWVDDRLLRRLAIKIGERIVLGEREFTVAAMV
                     SEEPESSVGFMNLGPRLMMNAADLSSTGLIQPGSRVSYRLFVAGDSKIIAAFRAFAVE
                     AIGAGQRVEDIRDARPEIKSALERAERFLGLAALMTVILAGVAVALAARRYLQRHLDA
                     CAIMRCIGATQALILRLHGLQFVMIGLIAGSVGCAVGLALQYVLAAVLAPVVSVTLPA
                     PGLLPLIQGLAAGFVMLLGFALPPLMALKQVPTLRVLRRDIGAPDPAGISAYVLGGGA
                     IASLVLWQAQEFRLGLYVLAGILGTAALAAALTVLVVQLLTRVGPSANLNWRFGVANL
                     RRHRAGSIVQVMALGLGLMALILLTLVRTDLLNSWRQSLPAQAPNRFLVSIQPDQVPA
                     VEQFFVDHNVPPPHLFPMVRGRLVEINGKGVSSRNYAEERARRLVDREFNLSWTKSMQ
                     PDNAIVEGRWFESRDTGKPLLSVEEGIARTLGVKTGDTLTYEIAGSRLTAQILSLRKV
                     AWDSFRVNFFVIAPAGLLEKFPASYVTSLHLSAERSVLLDELVQRFPNLIVIDVEAIL
                     SQVQRMMDQVVRAIEFVFLFSIAAGLLVLFAALGSTHDEREFDAAVMRTLGASGAQLR
                     AVQAAEFALIGALAGLLAALGATLIGYVLADRVLNLPFAINPWVWLAGLLLGAAGVTL
                     AGLLGTAKVLRTPPMQVFRASA"
     gene            2276..>2523
                     /locus_tag="VN496_15280"
     CDS             2276..>2523
                     /locus_tag="VN496_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXF18369.1"
                     /translation="MTRNVRNIAIIAHVDHGKTTLVDKLLRQAGTFAAHQHIAERVMD
                     SNDLERERGITILAKNCAVQYQGTHINIVDTPGHADFGG"
BASE COUNT          447 a          718 c          788 g          570 t
ORIGIN      
        1 gatgtggaac ctcgccgcat tccgtccacg ggtgaggtat gggtggacga tcggttgctg
       61 cgcaggctcg cgattaaaat cggtgagcgc attgtgctcg gcgaacggga attcacagtc
      121 gctgccatgg tcagcgaaga accggaatcg agtgtcgggt tcatgaatct cggcccccgg
      181 ctgatgatga atgcggcgga tctttcttcc accggattga tccagcccgg cagccgagtt
      241 tcctatcggc tgttcgttgc cggcgacagc aaaataattg cagcgtttcg cgcgtttgcg
      301 gttgaagcaa tcggcgcggg acagcgcgtg gaagatattc gcgatgcgcg cccggaaata
      361 aaatctgcat tggaacgcgc cgagcgtttc ctcggcctag ccgcactgat gaccgttatc
      421 ctggccggcg ttgccgtggc gcttgcggcc cggcgttacc tgcagcgaca tctcgatgct
      481 tgcgccatca tgcgctgcat tggcgccacg caggccctga tcctgcgcct gcatggattg
      541 cagttcgtga tgatcgggct gatcgcagga tcggttggtt gcgctgttgg cttggcgttg
      601 caatacgtat tggcagccgt gctggcaccc gttgtctcgg taacattgcc cgcgcccggt
      661 ttgcttccgt tgatacaggg gctggcggct ggtttcgtga tgctgctcgg gtttgcgctg
      721 cctccgctca tggcactgaa acaagtaccg acattgcgcg tgctacgccg ggatatcggt
      781 gcgccggatc ccgccggcat ttcagcctat gttcttggcg gcggcgcaat tgcgtcgctg
      841 gtgttgtggc aggctcagga attcaggctc ggtctttacg ttctggccgg cattctgggc
      901 acggcggcac tcgccgctgc cctcacagtg ctggtggtgc aactgttaac gcgggtgggc
      961 ccgagcgcaa atctcaactg gcgattcggt gtcgccaatc tgcggcgcca tcgcgccggc
     1021 agcatcgtgc aggtaatggc tttgggactc ggattgatgg cgcttatttt gctgactctg
     1081 gtgcgtaccg atctgctgaa cagctggcgg cagagcctgc ccgcacaagc gcccaaccgt
     1141 tttctggtca gcatccagcc cgatcaggtg cccgcggtcg agcagttttt cgtcgaccac
     1201 aacgttccac cgccgcattt atttccgatg gtgcgcggcc ggctggtgga aatcaacggc
     1261 aagggcgttt catcgcgcaa ctacgccgaa gaaagggcgc gcaggctggt tgaccgggaa
     1321 ttcaatttgt cctggacaaa gtcgatgcag ccggacaacg ccattgtcga gggccgctgg
     1381 tttgaaagcc gcgacaccgg aaaaccactg ctttctgtcg aagaaggcat cgccaggacg
     1441 ctcggggtga aaaccggcga cacgctgacg tatgaaatcg ccggctcgcg tttaaccgcg
     1501 caaatattgt cgctacgcaa ggtggcgtgg gactcgttcc gggtgaattt ttttgttatc
     1561 gcgcccgcgg ggctactgga aaaattcccg gccagctacg tgaccagcct gcatttgtcc
     1621 gccgagcgtt cggtgcttct cgacgaactg gtgcagcgct ttccaaacct gattgttatc
     1681 gatgtggaag ccatcctgtc gcaggttcag cgcatgatgg accaggtcgt gagagcaatc
     1741 gaattcgtct ttttgttcag tattgcggcc ggattgctgg tgctgttcgc tgcgctcggc
     1801 agtacgcatg atgaacgcga attcgatgcg gccgtcatgc gcacgcttgg cgcctcgggt
     1861 gcacagttgc gcgcggtcca ggcagcggag tttgcattga tcggcgccct ggcgggcctg
     1921 cttgcagcgc tcggagcaac cttgataggt tacgtgctgg ccgaccgggt gttgaatctg
     1981 ccgtttgcca tcaacccgtg ggtctggctg gcgggcttgc tgctgggcgc cgctggcgtg
     2041 acgctggcgg gattgctcgg tactgccaaa gtgctgcgca ctccgccgat gcaggtgttc
     2101 agagccagcg cctgattcca attgccgcct tgctttcaaa ctgcccgccg ttgaggcaac
     2161 ggatgggcag tgccttttgt gcgacgcgtt cgggtaaaag cattagacga atattcaagg
     2221 aattgcactc gttcctgtgg tacacttcgc gacctttttc gccgcactgc atttcatgac
     2281 acgtaacgtt cgcaacatcg ccattattgc ccacgtcgac cacggcaaaa ccacgctggt
     2341 cgacaagttg ttacgccagg ccggaacctt cgcggcgcat cagcatatcg ccgagcgggt
     2401 catggactcg aatgacctgg aaagagagcg cggcatcacc attctggcca agaattgcgc
     2461 ggttcagtac caaggcacgc acatcaacat cgtggacacg ccgggacatg ccgatttcgg
     2521 cgg
//
LOCUS       DATMZQ010000479         9736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_956968, whole genome shotgun sequence.
ACCESSION   DATMZQ010000479 DATMZQ010000000
VERSION     DATMZQ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956968"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..524)
                     /gene="rimM"
                     /locus_tag="VN496_15285"
     CDS             complement(45..524)
                     /gene="rimM"
                     /locus_tag="VN496_15285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXF18370.1"
                     /translation="MGRISAPYGLKGWIKIRPFTHVVDGLLSYAEWWIGDQGEWQPHR
                     VSESFVHGSSVVARLEGFNDRDAAARLKGRDVAVPRESMPEVREAEFYWNDLLGMDVM
                     DAKAARLGRVTKILETGANAVLVVTGDGEVLIPFIEGVIVQVDLKSRKLSVDWNQTE"
     gene            complement(538..792)
                     /gene="rpsP"
                     /locus_tag="VN496_15290"
     CDS             complement(538..792)
                     /gene="rpsP"
                     /locus_tag="VN496_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXF18371.1"
                     /translation="MVVIRLSRGGAKKRPFFNMVVADSRDPRDGRFIERVGFYNPKAP
                     EGQETLRIQMDRLRYWQGKGAKLSDTAARLVKQFGGTAQT"
     gene            complement(923..1696)
                     /locus_tag="VN496_15295"
     CDS             complement(923..1696)
                     /locus_tag="VN496_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18372.1"
                     /translation="MRSLVAFLFISVFSSAVCAQQPDYEREKRWSDQIVPAILVGDVV
                     WLQQKGGHKFLAIYTQAEKPRGAVIIGHGRGWNPDFELYGVLRVKLAEQGYTTLAIQL
                     PVLGPGAKVGDYLPTYPDAEERYALAAKFLQEKGFEKIAIVSHSLGATMANQYLIHAN
                     STPIKAWAFIGIINGLEEMFRIKIPVLDVFGGKDWEITQVGAYERKKQIMKVPGSDQV
                     IVPNAQHFFEGREDDLVKVLVEFLDSRFGSVNAASAGSK"
     gene            complement(1794..3140)
                     /gene="ffh"
                     /locus_tag="VN496_15300"
     CDS             complement(1794..3140)
                     /gene="ffh"
                     /locus_tag="VN496_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXF18373.1"
                     /translation="MLDNLTNRLAGVVKTLRGHARLTDSNIQEALREVRIALLEADVA
                     LPVVKDFIARIKAKAVGEDVIGSLTPGQALVGVVHDELTALMGSRNDALSFATVPPAV
                     ILMAGLQGAGKTTTAGKLAKLLLEQKKKVLLVSCDVYRPAAIDQLATLARQVGVEVFP
                     SASTQAPKDIAVAAFDYARKHYHDVMIVDTAGRLAIDDAMMREIALLHEVLQPIETLF
                     VVDAMQGQDAVNVAKVFSEMLPLTGVILTKLDGDARGGAALSVRHVTGRPIKFVGVSE
                     KLTGLEPFYPERLAARILGMGDVLALVEEARRNVDIGEAEKLAKKVKSGKGFDLDDFR
                     NQMEQMKKMGGLSALMDKLPAKLAQAAQGRMVDERVIGRTVGIINSMTPAERARPEII
                     KASRKRRIATGAGATVQEVNRLLSQFEQMQKMMKIMGKGGLQKMMRSVKGGFPGLH"
     gene            3222..4043
                     /gene="ccsA"
                     /locus_tag="VN496_15305"
     CDS             3222..4043
                     /gene="ccsA"
                     /locus_tag="VN496_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXF18374.1"
                     /translation="MKDILLHLLVSLGYAGLAIFLWRDALMPSTVQYGQLKASRAHLA
                     LIAPLILHAVLLVNSVFSGEDVYLGVGTAVSVIIWLTVLIYWTGGFFYRLEGLQVLVL
                     PSAAGLSLLPLILPSVRPLANTHLAAFKIHLVISLLAYSLFTIASLHVLMMAIMERRL
                     HRGNLPRFMQHLPPLLTMERLLFQIISAGFILLTLTLGSGILFSEELFGKPMQFTHKT
                     VFGILSWIIFGAMLSGRALYGWRGRVAMRWTLAGFLSLFLAYIGSKFVLEVLLQR"
     gene            4148..5416
                     /locus_tag="VN496_15310"
     CDS             4148..5416
                     /locus_tag="VN496_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HXF18375.1"
                     /translation="MDEIPLSSLLIAIVILLVISAFFSISETSMMALNRYRLRHLTKT
                     GHRGARIASQLLAQVDRLLGVVLLGNTLVNAGATTLATVIAIRLFGGGEIVLSLSTAV
                     VAFMLLVFSEITPKIIGATRPETIAFPAGYILAPLLKIAYPVTWFVNLFVRGLLWLVR
                     FHPNSNQPHTLSMEELRTLILEGGNFLPQKHQNILMNLFDLESITVEDAMTPRNQIEA
                     IDIDSPPDEIRRQIAISNHTRLPVYRERLDDIVGIVHVRKVLNLSEGEQIDAKTLTDV
                     LREPYFIPMGTPLLTQLQNFQEQQDRMGLVVDEYGELKGLLTLEDILEEIIGEFTTQS
                     PLQSARFRRMEDGSIIVEGATLLRDMNRKLGYKFPLDGPKTLNGLILEHFQDIPESGT
                     SLKLAGYPIEVLQTQDRVIKSIRLLPPDNG"
     gene            5565..6788
                     /locus_tag="VN496_15315"
     CDS             5565..6788
                     /locus_tag="VN496_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXF18376.1"
                     /translation="MATAAATRDRVKEYTFLWEGKDKGGKLVKGDMRAGGEHVVLAQL
                     RRQGIAVLKVKRQRGGLGGKVTQKDITLFTRQLATMMKAGVPLLQSFDIVGKGHANPA
                     VGKLLMDIKNEVETGNSLTNAFRKYPLYFDALYCNLVGAGEQAGILDSLLDRLATYKE
                     KILAIKSKIKAALFYPISIIVVAFIITAIIMIFVIPAFKDLFSSFGANLPAPTLFVMA
                     ISDFFVQYWWAIFGSVGFGVWFFFYTWQRSVKMQNVMDRLFLKIPVFGPLIRKATIAR
                     WTRTLSTMFAAGVPLVEALDSVAGAAGNQVYFEATKKVQQEVSAGISLTVAMTNTNVF
                     PNMVIQMVSIGEESGSLDGMLSKVADFFEQEVDDAVDALASLMEPMIMVVLGVLIGGM
                     VIAMYLPIFKMGQAV"
     gene            6873..7763
                     /locus_tag="VN496_15320"
     CDS             6873..7763
                     /locus_tag="VN496_15320"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXF18377.1"
                     /translation="MSIIEVLRNQPIMLTIVCGLIGLVVGSFLNVVIHRLPKMLDVEW
                     DNQAADLLEHKNMAEVAKRLRPRGTVRYNLMTPASTCPHCGHKIKAYENIPIISYLLL
                     RGRCASCKAPISIRYPIVEAISGLMAAYIGWKFGFTLSMAGALVFCLALLALTVIDID
                     TQLLPDDITLPLMWTGLLLNLNNLFAPLSSAVLGAAAGYLSLWSVYWLFKITTGKEGM
                     GYGDFKLLAAIGAWLGWKMLPVVILLSSLVGAIVGIGLIMLTRHGRNTPIPFGPYLAA
                     AGLIALFWGESINRTYLQVF"
     gene            7768..8391
                     /gene="coaE"
                     /locus_tag="VN496_15325"
     CDS             7768..8391
                     /gene="coaE"
                     /locus_tag="VN496_15325"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437624.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXF18378.1"
                     /translation="MAFTVGLTGGIGSGKTTVAELFASNGAGVVDTDEISHRLTSPGQ
                     PAVAEIARKFGPQFFATDGSLDRPRMRSLVFSDPAARKNLEAILHPLILAETARQIQN
                     AIAPYVIVVVPLLIESGSYRKTVDRILVVDCEPETQIRRVIERSGLVRNEVLSIIATQ
                     VSRERRLTEANDIILNEGPLDSLMLQVKAFHMQYLRLAKSKIGSDPD"
     gene            8470..9222
                     /gene="zapD"
                     /locus_tag="VN496_15330"
     CDS             8470..9222
                     /gene="zapD"
                     /locus_tag="VN496_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HXF18379.1"
                     /translation="MISYEFPLNERIRTLLRLEDLYRRTQHFASKTESEEHHVALTSL
                     FELLDVGGRADLKSDIMQELERQKQHLEALHNNPAISQEALTTVLSDIDKAVSDLFLS
                     SGKTGQELRENDWLMGIKQRSAIPGGVCEFDLPSYHYWLNRSAEARRLDLDQWLAPFI
                     PIRNGVRIILKLLRENGKTAKQIAHQGVYQQTMAGRLAQMLRIRLDSSYDCVPEISAN
                     KYALNVRFTTAVGTQRRTTVEEIGFELTFCNL"
     gene            9222..9425
                     /locus_tag="VN496_15335"
     CDS             9222..9425
                     /locus_tag="VN496_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HXF18380.1"
                     /translation="MPSESRKARKVHCPQCNASIVWSIENPYRPFCSQRCKLIDLGQW
                     ASGAYRIPQVERDSGRTDDEENS"
     gene            complement(9422..>9736)
                     /locus_tag="VN496_15340"
     CDS             complement(9422..>9736)
                     /locus_tag="VN496_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXF18381.1"
                     /translation="SRELMGTKVRPDVEWCSASCHNSAELDKARDLGLDFVLLGPVEQ
                     TSTHADAAGMGWKNFESLVQNYSLPVFALGGMRSENLDAARLRGAHGIAMLRGAWDQS
                     ER"
BASE COUNT         2222 a         2612 c         2484 g         2418 t
ORIGIN      
        1 ctccgggaat agtgtgacta cgtcgaaatg catctcttta ccaattactc ggtttggttc
       61 cagtccactg ataactttct ggacttcaaa tctacctgaa cgataacgcc ttcgataaaa
      121 gggataagaa cttctccatc acccgttacg acaagcacgg cgttagcgcc cgtttccaga
      181 atttttgtga ctcgccccag tctggcagct ttcgcatcca tgacatccat cccgagaagg
      241 tcgttccaat aaaactctgc ttcccgaact tcaggcattg attcgcgggg aactgcaacg
      301 tccctgccct tcaaccgtgc ggccgcatcc cgatcattaa atccctctag ccgcgcaaca
      361 accgaagacc catgcacaaa cgattccgat actcgatgcg gttgccattc tccttggtcg
      421 cctatccacc attcagcgta gctcagcagt ccgtccacaa cgtgcgtaaa gggccggatt
      481 ttaatccagc cctttagacc gtatggggcg cttatgcgcc ccataatgac cattgtctca
      541 ggtctgtgct gtaccaccga actgctttac cagacgtgcg gccgtgtcgg aaagcttggc
      601 gccttttccc tgccagtacc gaaggcgatc catttgaatg cgcaacgtct cctgaccttc
      661 aggagccttg gggttgtaga accccacgcg ttcaatgaag cgaccatccc ggggatcgcg
      721 cgagtccgcc acgaccatgt taaaaaatgg acgcttcttg gcccctcccc gcgaaagtcg
      781 aatgactacc atgtgcttgt ccttaaaata cgaagcccgc gattctacgc cggttttgga
      841 cgtatcggca agcgcttggg ttgatccacg cccatcctcg atactgcaaa tgtccgggcg
      901 tataaacgtg tgctcccctc agctacttcg atcccgctga tgcggcattg acagagccga
      961 agcggctgtc cagaaactcc accagtactt taaccaggtc atcctcacgg ccctcgaaaa
     1021 aatgctgggc attcggaaca atcacctggt cggatcccgg caccttcatg atttgttttt
     1081 tccgctcata cgctcctact tgtgtgattt cccagtcctt gccaccaaat acatccagta
     1141 ccgggatttt gatgcggaac atctcctcga ggccgttaat gattccgatg aatgcccagg
     1201 ctttgatggg cgtggaattg gcatggatca ggtattggtt cgccattgtc gcgccgagac
     1261 tgtgcgacac gattgcaatt ttctcgaagc ccttctcctg cagaaatttg gcggccaatg
     1321 cgtaccgctc ttcggcatcg ggatacgtcg gaagataatc gccaactttg gccccggggc
     1381 ccaatacggg caattggatc gcgagcgtgg tatagccttg ctccgcaagc ttgacgcgca
     1441 atacaccata gagttcgaag tcgggattcc aacctcttcc atggccgatg attaccgcac
     1501 cgcgcggctt ctccgcctgg gtataaatgg cgaggaattt gtggccaccc ttttgttgca
     1561 gccagacgac gtcgccaaca agaatggccg ggacgatctg atccgaccaa cgtttttcgc
     1621 gttcgtaatc gggttgctgc gcacaaacag ccgatgaaaa aacactaatg aacagaaacg
     1681 cgacaagcga gcgcatgtgg ttaccttaaa cgttctataa ctcgaaacat caatcgtgaa
     1741 tgagcaactg ctcagccata ctttgtgact gccatggcgg gcgtgcgttc acattaatgc
     1801 agacctggga agccgccttt tactgaacgc atcatttttt gcaaaccgcc tttgcccatt
     1861 atcttcatca ttttctgcat ctgctcaaac tggctgagca ggcggttgac ttcctgcacg
     1921 gtagccccgg ctcccgtggc tatgcgccgc ttacgagagg cctttatgat ttccggcctc
     1981 gcacgttccg cgggcgtcat ggaattgata atacccactg tgcgccctat cacccgctcg
     2041 tccaccatac ggccttgagc agcctgtgcc aatttcgcag gcagcttatc catcagtgcg
     2101 ctcaggccgc ccatcttttt catttgctcc atctgattgc ggaagtcgtc cagatcaaag
     2161 ccctttccag acttcacttt tttggctagt ttttcggcct ctccgatatc gacgttgcgt
     2221 cgcgcctcct ccaccaatgc caacacatcg cccatgccca aaatccgtgc cgcaagacgt
     2281 tctggataga aaggctccag accggtgagt ttttccgaca cgccaacaaa cttgatcgga
     2341 cgaccagtga catggcgcac ggacaacgca gcaccgccgc gggcatcgcc atcgagcttg
     2401 gtgagaatta caccggtcag aggcaacatc tcggaaaaaa ccttcgccac attaaccgca
     2461 tcctgtcctt gcattgcatc gaccacaaaa agcgtttcaa tcggctggag cacttcgtgc
     2521 agcagcgcaa tctcccgcat catcgcatca tcaattgcaa gccggcctgc agtatcaacg
     2581 atcatgacat cgtgataatg tttgcgcgca taatcaaacg cggccacggc gatgtctttt
     2641 ggcgcttgcg tagaggcaga cggaaatact tccacgccca cctggcgtgc aagcgtagcg
     2701 agttggtcta tggccgcagg gcgatatacg tcgcaactaa cgagcaacac ttttttcttt
     2761 tgctccagga gcaacttcgc caatttacct gcggtagttg tcttgcccgc accttgcagc
     2821 cccgccatga ggattacagc gggaggaact gttgcgaagc ttagcgcgtc attgcgacta
     2881 cccattagcg cagtgagttc atcgtgcacc acgcctacca atgcttggcc tggcgtaagg
     2941 ctcccgatca cgtcctcgcc taccgccttg gctttgatcc gggcaatgaa atcctttact
     3001 accggcagcg ccacatcggc ttccagcaac gcgatgcgca cctctcgcag agcctcctgg
     3061 atattagaat cggtcaggcg cgcatgccct cgcagcgtct tgacgacgcc ggctagccga
     3121 ttggtaaggt tgtctaacat tttgatgtcc ggatgtgttg atggccggat gtggtcagcg
     3181 cggggatgtt gtagacttgg ccagcaattc ttcgcctcag aatgaaagat attctacttc
     3241 atctgctcgt aagtctcgga tatgcgggac tcgcaatctt cctttggcgt gatgcgctca
     3301 tgccatccac tgtgcaatat gggcaactga aggcatcaag ggcgcacctt gcgcttatag
     3361 caccgcttat cctgcatgcc gtgctgctcg tgaattctgt cttttccggt gaagatgtct
     3421 atcttggcgt tggaactgcc gtttcagtga tcatctggct taccgttctg atctattgga
     3481 ctggaggttt tttttacagg ttggagggtc tacaggttct tgtcttgccg agcgcagcag
     3541 gactttcgct tctgccgttg attctgccaa gcgtgcgtcc gctggccaat acacatctgg
     3601 cggcattcaa gatccatctg gtgatctcac tgctggctta cagtcttttt acgatcgcct
     3661 ctttgcacgt gttgatgatg gcgatcatgg aacgaaggtt acatcgggga aatttgccaa
     3721 gattcatgca acatttaccg ccgctgctga cgatggaaag gcttctgttc cagatcatat
     3781 cggcaggatt tatccttctt acgttgactc tcggaagtgg cattctattc tcggaggaat
     3841 tgttcggcaa accgatgcag ttcactcaca aaaccgtttt tggcatttta tcgtggatca
     3901 ttttcggcgc gatgctgtca ggccgcgcac tttacggatg gcgaggccgc gtcgcgatgc
     3961 gctggactct ggcgggattt ctctcgcttt ttctggctta catcggcagt aaatttgttt
     4021 tggaagtatt actgcagcgc tagtcgcatg aagcgctagt cccatgagac gtggcccatg
     4081 cttgcttgac accgcggttg cctggtgcca atactaattt tcttaccaga cgatggagtg
     4141 acagtacttg gatgagattc cactttccag tctgctgatc gcaatagtca tcttactcgt
     4201 catctcggcg ttcttttcaa tttccgaaac cagcatgatg gcgctcaatc gctatcgatt
     4261 gaggcatctg accaagacgg gtcatcgagg ggcgcgaatt gcttctcagc ttctagcgca
     4321 agtcgatagg ttgcttggcg tagtgttgct tggtaatact ctggtcaatg cgggcgcaac
     4381 tacgcttgct acagttatcg ccattcgcct gttcggcggc ggggagatcg tgctgtccct
     4441 gagcacagcc gtagtggcct tcatgcttct ggtattcagc gagatcacgc ctaaaatcat
     4501 tggcgcaacg agaccagaaa ccatcgcttt ccccgccggc tacatcctcg ctcctttact
     4561 gaagattgct tacccggtca catggttcgt aaaccttttt gtgcggggac tactctggtt
     4621 ggtccgtttt catccgaatt ccaatcaacc gcatacgtta agcatggagg aattgcgcac
     4681 tcttattttg gagggaggca atttcctgcc acaaaagcat cagaacatac tgatgaacct
     4741 gttcgatctt gaatcgatca ccgtggaaga cgccatgacg ccaagaaacc agattgaggc
     4801 gattgatatc gattcgccgc ccgatgagat acgccggcag atcgcgatca gtaaccacac
     4861 gaggctgccg gtctatcgag aacgcttgga cgatattgtt ggcattgtcc acgtgcggaa
     4921 ggtgctgaac ctgtccgaag gcgagcagat tgacgccaaa acgctaaccg atgtcctacg
     4981 cgaaccgtat ttcatcccga tgggaacgcc tttgctcaca caattgcaga acttccagga
     5041 acagcaagac cggatggggc tcgtggtcga tgaatatggt gaactgaaag gtctgcttac
     5101 actggaagat attctcgaag agatcattgg agaatttacc acccagtctc ccctgcaaag
     5161 tgcccgtttc cgccgcatgg aagacggcag catcatcgtg gagggtgcaa ccctgttgcg
     5221 cgacatgaac aggaaactcg gctataaatt tccactggac ggaccgaaaa ccttgaatgg
     5281 gctcatcctt gagcacttcc aggacattcc ggagtcgggt accagcctaa aactcgccgg
     5341 ctatccgatt gaagttctcc aaacccaaga ccgcgttatc aaatcgatcc ggctcctacc
     5401 gccggataat ggctaaaaat cagcgggatt ttgcgtttac ttccaccttt acttcaacct
     5461 tcggccagtg catcattgtc agtctgaatg gctagaaggc atgcggacac tatcccaccg
     5521 cgtccatcct ggggtacgac tgtgagagtg aagaggtaag cactatggcg acggctgcgg
     5581 caactcgaga ccgggtaaag gagtacacct tcctctggga aggcaaggat aagggcggaa
     5641 aactggtcaa aggcgatatg cgtgctggcg gcgaacacgt cgttttagcc cagcttcgcc
     5701 ggcaaggcat cgcggtactc aaggtaaagc gccagagagg tggccttggc ggcaaggtta
     5761 cacagaagga catcaccctc tttacgcggc aattggcaac catgatgaag gctggcgtac
     5821 ctctgctcca gtcctttgac atcgtcggaa aaggtcacgc caacccggcg gtgggcaaat
     5881 tgctgatgga catcaaaaac gaagtcgaaa cgggcaacag cttgacgaat gccttccgta
     5941 aatatccgct ttatttcgat gcactgtatt gcaatctggt aggcgcggga gagcaagccg
     6001 gtattctgga cagtcttctc gaccggctgg cgacttacaa agaaaaaatc ctggcaatca
     6061 aaagcaagat caaagctgcg ttgttctatc ccatatcgat catcgtggtg gcattcatca
     6121 tcaccgcgat catcatgatt ttcgtgatac ccgcattcaa ggatctgttc tccagttttg
     6181 gagcaaactt gcctgccccg acactgtttg tcatggcaat ttcagacttc tttgtgcagt
     6241 attggtgggc gatcttcggt agcgttggct ttggggtatg gttctttttc tacacgtggc
     6301 agcgatccgt aaaaatgcag aatgtcatgg atcgtttgtt tctcaagata ccggtattcg
     6361 gaccattgat acggaaggcg accattgcgc gctggacacg gaccctttcg acaatgtttg
     6421 cggccggtgt acctttagtc gaagccttgg actcggtggc cggtgccgca ggcaatcagg
     6481 tttactttga ggccaccaag aaggtacagc aggaagttag cgccggtata agtttgactg
     6541 tggcgatgac caataccaac gtattcccga acatggttat ccaaatggta tccatcggcg
     6601 aggaatcagg atccctggac ggaatgctaa gcaaagtagc tgacttcttt gagcaggaag
     6661 tcgatgatgc ggtagacgcc ctggccagtc tgatggaacc catgatcatg gtcgtgctag
     6721 gggtattaat tgggggaatg gttattgcaa tgtacctgcc gatcttcaag atgggtcagg
     6781 cagtataaaa ggattttgac gataaagcgc ccgacgaact cgggcgcttt tttttatcat
     6841 cgcttgctct tcgaatcatc ggtgttccat tcatgtcaat aatcgaagtg cttaggaatc
     6901 aaccgatcat gctgacaatt gtatgcgggt tgattggact ggtggtcggc agctttttaa
     6961 acgttgtcat ccaccggctt ccaaaaatgc ttgatgtcga atgggataac caggcagcgg
     7021 atctgctcga acacaaaaat atggcagagg tggcgaaaag acttcgcccg cgcggtacgg
     7081 tgcgctataa cctcatgact cccgcctcga cttgcccgca ttgcggacac aagatcaagg
     7141 cgtacgagaa tattccaatt atcagctatc tcctgctgcg tggccgctgc gcgtcatgca
     7201 aggcgccgat tagcattcgc tatccgatcg ttgaggcaat aagcggactt atggccgctt
     7261 atataggctg gaagtttgga ttcacccttt ctatggcggg agcgctggtg ttttgcttag
     7321 cgctcctggc acttacggta atagatattg acacccagct gcttccggat gacatcacct
     7381 tgccgctgat gtggactggg ttgctgctca atttgaataa cctctttgcg ccgctttcat
     7441 ccgcagtact cggcgcggcg gcaggatatc tttctttgtg gtcagtctat tggctgttca
     7501 aaattactac cggaaaggaa ggcatgggct acggagattt caagcttttg gccgctatcg
     7561 gtgcgtggtt aggatggaag atgctccccg ttgtcattct gctttcttct ctggtgggag
     7621 caatagtcgg aattggttta atcatgctga cgcggcatgg tcggaatact cccattcctt
     7681 ttgggcctta cctcgccgcg gcgggactga tagccttatt ctggggcgaa tccatcaatc
     7741 gtacctattt gcaggttttc tagagcaatg gcattcactg ttggcctgac cggaggtatt
     7801 ggatcgggca aaacaacggt ggcggaatta tttgcatcga acggggctgg tgttgttgat
     7861 acggatgaaa tatcgcaccg cctcacaagt ccgggccagc cagcggttgc agagatcgcc
     7921 cggaagtttg gaccgcagtt tttcgccact gatggaagcc tggatcggcc aaggatgcgt
     7981 agtctcgtat tttccgatcc agcggcccgc aaaaatttgg aagcaatctt gcatccatta
     8041 atcctggcag agaccgcgcg ccagatacag aatgcgattg ctccctacgt cattgtggtg
     8101 gtaccgcttc ttattgaatc aggttcttat agaaaaaccg tggatcgaat acttgtagta
     8161 gattgcgagc cggaaaccca gatccgcagg gttattgaac gcagcggtct agtccgcaat
     8221 gaggttttgt ccattatcgc gactcaagtg tcaagggagc ggcgactgac tgaggcaaac
     8281 gatattattc ttaacgaagg cccactggat tcgttaatgc ttcaagtaaa agcatttcat
     8341 atgcagtacc tacgactcgc gaagtcaaaa atcggatccg atccggattg attgcctgga
     8401 tcggccaagg ctttattttt tgattctttt cagcaatact tgcccaactc ctgtggccac
     8461 ctccacatcg tgatcagcta cgagttccca ctcaacgaga ggattcgcac cctgctgcgc
     8521 ctggaggacc tataccggcg cacgcagcat ttcgcctcca aaactgaatc tgaagagcac
     8581 catgttgctc taacgtcgct gtttgagttg ttggacgtgg gaggccgcgc ggatcttaaa
     8641 tcggacataa tgcaagaact ggagcgccag aaacagcatc ttgaggccct tcataataat
     8701 cccgctattt cccaggaagc tttgaccaca gtactctcgg atattgataa agcggtttcc
     8761 gacctgttcc tttcgtctgg caagaccggg caggaacttc gagaaaatga ttggttaatg
     8821 gggatcaagc agcgttccgc aatacccggg ggcgtgtgcg aatttgattt gccctcctat
     8881 cattactggc ttaaccgaag cgctgaagcg cgccgcctgg atctggatca gtggcttgcc
     8941 ccatttatac cgattcgaaa tggcgtccgg atcatactca agctgctaag ggaaaacggc
     9001 aagactgcaa agcagatcgc ccaccagggt gtctatcaac agaccatggc agggaggttg
     9061 gctcagatgc tccggattag gctcgatagc agttacgatt gcgtccctga gataagcgca
     9121 aacaaatatg ctcttaatgt ccgctttacg acagcggtgg ggacacagcg ccgaaccact
     9181 gtagaagaaa tcgggtttga acttacgttc tgcaatttgt aatgccgtcc gaatccagaa
     9241 aagcgcgcaa ggtacattgt ccgcaatgca atgcctcaat agtttggagc atcgagaatc
     9301 cgtaccgtcc attctgttct cagcgctgca aactgatcga tctggggcag tgggccagtg
     9361 gcgcataccg aattccacaa gtggaacgcg atagtgggcg gaccgacgac gaagagaact
     9421 cctagcgttc ggattgatcc caggctcccc gcaacatcgc gataccatgc gctcccctga
     9481 gccgcgccgc gtccagattt tctgatcgca tccctcctag agcaaacacg ggaagcgaat
     9541 aattttgaac caggctttca aagtttttcc aacccattcc ggccgcatca gcatgggttg
     9601 acgtttgttc aaccgggccc aggaggacga agtccaatcc cagatcacgt gctttgtcca
     9661 gttctgcact gttatggcag gaggcgctgc accactcgac gtcaggccga accttggtac
     9721 ccatcaattc gcgtga
//
LOCUS       DATMZQ010000480         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_968956, whole genome shotgun sequence.
ACCESSION   DATMZQ010000480 DATMZQ010000000
VERSION     DATMZQ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968956"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="VN496_15345"
     CDS             complement(<1..1874)
                     /locus_tag="VN496_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HXF18382.1"
                     /translation="MAAKLYRTHAVFRNAMDQCHALAEPYLEQGLREVIFAGDRDTAL
                     VDRTDYTQPALFAVEHALAELLKSWGITPAAVIGHSLGEITAAFAADVISLEDAMRLV
                     TARGALMHRLPGGGGMAAIFAEESVVRTLINTIAPGLAVAAMNGPLNTVVSGDRDAVR
                     MLLEELDRQGIKYRELQVSTAFHSPLTELILDDLENVAGQIKHNAPKLPLISNLTGGP
                     MSTAPDKIYWRRHAREAVRFGDGMLALAQLECRTFLEVGPHPVLLPLAQVCLGANGKS
                     AAWVATLNRQKPDADSITEMLVALYLAGHNVNWTAVHADSSWRRIPLPTYPFQRQRHW
                     IEDNTIHTEKRVRNTVERLHPLVGTRINSTAKEVCYEARYGVHHAGYLSDHRVAGAIV
                     LPTTAELEAATAVGRMQFGTSRVSFDDAMHHQAMAFANGEDRIVRVLVTPLKSDRAGF
                     RLVSAAAEGSDVWHTHMTGTLRKSDVPSRQAFSTKQVRARCQQTLPVADFYDRLGGLG
                     LEYGPSFRGVRELYVGQHEALTKVGLPDGLANTQYAMHPAFLDACLHAYPFVLDVAEN
                     AASDGRKSYLPVSLAGFRCYQDEIDEAWVHTTLRSVEKDDTQVVDIRVYDMAQRPVAE
                     L"
BASE COUNT          506 a          701 c          666 g          486 t
ORIGIN      
        1 agttccgcca ccggccgctg cgccatatcg taaacgcgaa tgtcgaccac ctgcgtatcg
       61 tctttctcga cactccgcag cgtagtgtgc acccatgctt cgtcgatctc gtcctgatag
      121 cagcgaaatc ccgccagcga gacaggcagg taggacttac gaccgtcgct tgcggcattc
      181 tccgcgacat cgagtacaaa aggataggca tgcaggcacg catccagaaa cgcggggtgc
      241 atcgcgtatt gcgtatttgc caaaccgtcc ggaagcccca ctttggtaag cgcctcgtgc
      301 tgaccgacgt acaattcccg gacgccacgg aagctggggc catattcgag ccctaacccg
      361 ccgagcctgt cgtagaaatc ggcaacaggc agtgtttgct gacaacgggc ccggacctgc
      421 ttcgtcgaaa aagcctgccg ggacggcacg tctgacttgc gcagggtgcc cgtcatgtga
      481 gtatgccaga cgtccgagcc ttcagcggcc gcactgacaa gtctgaaacc tgccctgtcg
      541 gatttcagcg gagttaccaa tacccgcaca atccgatctt ccccattggc aaatgccatc
      601 gcttggtgat gcatcgcatc atcgaaactc acccgcgacg ttccgaattg catacggccg
      661 acggccgtgg cggcctccag ttcagcagtg gttggcagaa cgatcgctcc cgcaactcta
      721 tggtcggaga ggtaaccggc gtgatgcacg ccgtagcgcg cttcataaca cacttctttg
      781 gcggttgaat ttatgcgcgt acccaccagc ggatggagtc gttcgacagt attccggact
      841 cgtttttcag tatggatcgt gttgtcttct atccagtggc gctggcgctg gaagggataa
      901 gtgggcaacg gtattcgtcg ccatgacgag tcggcatgga ctgcagtcca attcacgttg
      961 tggccggcga gatacagcgc gaccagcatt tcggtgatgg aatcagcgtc cggcttctga
     1021 cggtttagcg tagcaaccca ggcagcggac tttccgttcg ctccaaggca cacttgcgcg
     1081 agcggcagca gcaccgggtg tggcccgacc tcgagaaagg tccggcactc cagctgagca
     1141 agagcaagca tcccatctcc gaaccgcaca gcttcccgag cgtgccggcg ccaatagatt
     1201 ttgtcgggcg cggtcgacat cggcccgccc gtcaggttgg atatcagggg tagtttgggc
     1261 gcgttatgct tgatctggcc cgcaacgttt tccagatcat caagaatgag ctcggtgagc
     1321 ggcgaatgaa aggcggtgga aacttgcaat tcccgatatt tgatgccttg cctgtccagc
     1381 tcctccaata acattctcac tgcatcccgg tcaccggaca cgacggtatt cagcggaccg
     1441 ttcatcgcgg ccacagcaag tccgggagcg atcgtgttga tgagcgttcg caccacagac
     1501 tcttcggcga atatcgcggc catacccccg ccgcccggta gtcggtgcat caacgcacca
     1561 cgagcagtga ccaatcgcat cgcgtcttcg agcgaaatga catccgccgc aaaagcagcg
     1621 gtaatttctc ccaagctgtg cccaatcact gcggcaggtg tgatacccca ggatttcaaa
     1681 agttctgcca gagcatgctc aactgcaaac agcgcaggct gggtgtagtc agtgcggtca
     1741 accagcgcag tatcgcggtc tcctgcaaaa atcacctcgc gcaagccttg ctcaagatac
     1801 ggctccgcca acgcatgaca ttgatccatc gcattccgga aaacggcatg tgtgcgatat
     1861 aacttcgcgg ccatgccggc gtactgcgaa ccctgaccgg aaaacatgaa cgcgatcggc
     1921 ccactgcttg tgcgctgcag actcgacgga tttccggcca cctttcgcag ccatgcttcg
     1981 aggtgctttt tcagctctgc gactgaccgg gcaggcgcgg cgaagcggaa tgtaaactgg
     2041 cttctgctaa cgttagtggt gtagcaaacg tcatcaattg atgcgtcggg attgtcactc
     2101 aaccagttcc gaagttgctc gacacgcgac accaatgcgt ctgcgctttt ggctgaaagg
     2161 gtcatcaaac aggggaagcg atctgatgac tgactcctgg ccggcgcgga tggcggcgcc
     2221 tcttcgagga ttgcgaaggt attcgtgccg ccaattccaa ggccattgag cccagctcgc
     2281 cgcggcgtgt ccgcacgagg aaaatcctgc agctttgtat ttacgtaaaa gggcgacgat
     2341 gagaaatcga ttcgggggt
//
LOCUS       DATMZQ010000481        11426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2293952, whole genome shotgun sequence.
ACCESSION   DATMZQ010000481 DATMZQ010000000
VERSION     DATMZQ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293952"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /gene="pilQ"
                     /locus_tag="VN496_15350"
     CDS             complement(<1..2021)
                     /gene="pilQ"
                     /locus_tag="VN496_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HXF18383.1"
                     /translation="MKGIAGVMNLAVVPTLLGLLLSASVYGQDKAAAPATTGNVIQSI
                     DVSSQPGAVTIKFGLKDAPTNPPAAFTVNNPPRVAFDFPGTTNGLGKNSQEVNQGDLR
                     TIRLGQSGGRTRVVLNLAKMVKYETKIEGNTVIITLQSGGTASASSTETAPSFAEPKA
                     GGEGHAVRDVDFRRGRNGEAQIIVDLSDATTGIDLRQQGKSILVDFIHAALPPSLERR
                     FDVSDFGTLVDSFDVIRSGGNTRITVAPKGRWEQSAYQTDNRFIIEVKPVIEEAGKPK
                     SAGGYTGDKLSLNFQNIEVRAVLQVIADFTSLNIITSDTVSGNLTLRLKDVPWDQALD
                     IILQAKGLDMRKTGNVVWIAPRDELATKEKLALESRQQISDLEPVRTESFQLNYQKAE
                     DFQKVITDPNQRILSKRGSAVVDARTNQIFVQDTPTKLEEVRELLKKTDRAIRQVLIE
                     ARIVEATDTFSRNIGTRLGYHDLTGRGSAAGSDHRALIGGGLADTGAHTGQTSNTPNF
                     ITDSLFSNMAAPGLGGVLPGQASLILMNDAMTKFLNLEITALQADGKGKIISSPRVIT
                     ADNAEATIEQGTEIPYQQATSSGATSVSFRKATLSLKVKPQITPDDNIIMKLNVNKDS
                     VGAATSAGPAIDTKQVSTEVLVENGGTVGLGGIYTQDESSTITKVPLLG"
     gene            complement(2040..2564)
                     /locus_tag="VN496_15355"
     CDS             complement(2040..2564)
                     /locus_tag="VN496_15355"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HXF18384.1"
                     /translation="MRLRSIFAIVALVLSGCGGEQFSDLKQFVKDSGEGLRGRVEPIP
                     EVKQFEPFAYNAFDLPDPFRPRKTLADTRSGAAGGSGPRPDLNRRKEALEAFPLESLQ
                     MVGTLEQKQVRFALIKTPDNNLYRVKFGNYLGQNFGVVTSVSESTVTLKEVIQDSSSG
                     SWTERTSSLQLLEQ"
     gene            complement(2564..3196)
                     /locus_tag="VN496_15360"
     CDS             complement(2564..3196)
                     /locus_tag="VN496_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXF18385.1"
                     /translation="MTLDDLRRLNPRDIGSWPALPKLGALFILLLLLVFAGYWFDWQN
                     QLETLARDRAKEQDLRTAFLDKKKQAVNLEAYRKQLAEIEESFGEMLKQLPNKSEMEA
                     LLTDINQAGLGRGLQFELFRPAPAETRSEFYAELPITIKLAGNYHDIGAFASDISQLS
                     RIVTLNDIALSSSKSGLTLDATAKTFRYLDDSEVAAQRKAARDAAAAKKK"
     gene            complement(3201..3806)
                     /locus_tag="VN496_15365"
     CDS             complement(3201..3806)
                     /locus_tag="VN496_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXF18386.1"
                     /translation="MIPINLLPHRAARRKAQQQQFFVLAGMTVGLAAAIVVAVHTFFA
                     GRIETQVERNKYLESEIVLLDKQIDEIRKLKEQTQALLARKRVVESLQTNRTETVRLL
                     DQLVRQLPDGIYLKAIRQTGQRVNVIGYATSNARVSTLMRNFEASPWLESPSLIEIKA
                     VTVDAAPLNEFNLNVNISRPKEENVPRPATGSPTAPATPKT"
     gene            complement(3803..4909)
                     /locus_tag="VN496_15370"
     CDS             complement(3803..4909)
                     /locus_tag="VN496_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HXF18387.1"
                     /translation="MFKGKIAFDADFIGNLLKPKSAPLIGVDISSSSVKMVELAGSGR
                     GRYRLERYTIEPLPKDAVVDGNIMNLEEVGDALRRGWKRMGSRAKNLAMALPSAAVIT
                     KKIIVPAGQTEEQLEAQVETEANQYIPFALEEVNLDFQTLGPAPNSTEDVEVLIAASR
                     KEKVEDRVAVAESVGLKATVMDIDSFASQNAFELMQGQLPDAGHDQTIAVVDVGASMM
                     NINVLRNGQSVYMREQPFGGNQLTQEIMRHYSLSVEEAEAAKRNGGLPDTYDSEVLAP
                     FMDTLALEIARALQFFFTSTQFNQVHHIFLSGGSATIPGLPEVVAERTQVNTLIANPF
                     VGMEVASKVRQAQLTIDAPALLVACGLALRRFDE"
     gene            5062..7395
                     /locus_tag="VN496_15375"
     CDS             5062..7395
                     /locus_tag="VN496_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HXF18388.1"
                     /translation="MIFSRWWIYPIIGFAALAVLLALVAGYAAAVAYPNLPSLDALTS
                     YQPKIPLQIFSMEGDLIGEFGEEKRAFVKIDQTPDLLRKAILAAEDERFYEHGGVDYI
                     GVMRAALSNFASASARQGASTITMQVARNFFLSTEKTFSRKFNEALLAFKIEHSLTKD
                     EILQLYINQIYLGQRAYGFGAASQVYFGKQLDQLSLAELAMLAGLPKAPSRYNPVVNM
                     TRAKIRQRYVLRRMADLGHINDDQLKKAESQLIVISRQVQEFSTRADHFAEMVRQAIF
                     DAYKEEAYTRGFRVYTTLSKLHQDAAYRALRQGVMDYDKRQGYRGAEGYFNLPKEEVS
                     EEALEDSLQEELESDDIYPAIALEIDPKAVKAYRKGGETLTITGDGLKFAGRMIGSKA
                     PAKTRLKRGSLIRVQRDAKGNWQIVQLPQVESALVSVDTDTGAIRALVGGFDFYRNKY
                     NHVTQARRQPGSSFKPFIYSAALEKGFTPATIINDAPLTIDASETGSVNWQPRNFDGS
                     FDGPIRMRTALTKSKNLVSIRILQAITPQYAQDYISRFGFDPKQHPAYLTMALGAGST
                     TPLEMVMGYSVFANGGYRMTPYFIERIEDTGGNVLGKANPVVVGKGAERVLDPRNAFI
                     MTNMMQDVIRGGTGARAMALGRIDLAGKTGTTNEQLDAWFAGFQRHLASVVWVGYDIP
                     RTLGDRETGAVVALPIWMSYVGVVLKGQPEELPIAPEGVVAVAVNPETGLRDKHAAAP
                     MIEYFFRENVPPEQEAGPEIEGGDTPKPQEEVKDQLY"
     gene            7467..8021
                     /locus_tag="VN496_15380"
     CDS             7467..8021
                     /locus_tag="VN496_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18389.1"
                     /translation="MAVDGIDDYALAKRKAARQAGAPETRNLPNNDEVEHALRAYQQL
                     YQADEQRARLLHLRKSARHMMELLARFNPYLSGAVLSGRAGKYSEIELLLFTDSMKDV
                     ELFLLNRRIVYSCGERRMRISDESRSVPVFRVGTDDADFDIAVLEPRDLRSQIRNAPE
                     GRPLERVRIDWLEAELAKPDGFAA"
     gene            complement(8028..9032)
                     /gene="hemB"
                     /locus_tag="VN496_15385"
     CDS             complement(8028..9032)
                     /gene="hemB"
                     /locus_tag="VN496_15385"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXF18390.1"
                     /translation="MQVLGRFPRRRMRRLRHDDFSRRLVREHRLSADDLIYPVFVVDG
                     QNKTEAVPSMPGVERMSTDRLLRRAEECVRFGIPAIALFPVVDHRLRTTDAKEAFNPE
                     GLAPKAIRALKKEFPELGVITDIALDPYTSHGQDGLVDAGGYVLNDETIQVLVKQALS
                     HAEAGVDIVAPSDMMDGRIFALREALDAAAHIHTRILAYAAKFASSFYGPFRDAVGSS
                     ATLGKANKYTYQVDPANSNEALWEVGLDLAEGADMVMVKPGMPYLDIVRRVKEEFGAP
                     TLVYQVSGEYAMLKAAGQNGWIDERKCVMESLLSFKRAGADAVLSYFALDAAMWLKEN
                     "
     gene            complement(9244..9849)
                     /gene="yihA"
                     /locus_tag="VN496_15390"
     CDS             complement(9244..9849)
                     /gene="yihA"
                     /locus_tag="VN496_15390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXF18391.1"
                     /translation="MSQSGLFRDIEFFGSAADETGLPPSKMEVAFAGRSNAGKSSAIN
                     ALARRHKLAFVSKTPGRTQQINFFSLGNERYLVDLPGYGYAKVPQKIKQDWEQFISSY
                     LLLRPQLRGLIVVMDIRHPLKEIDHQLLSWYGSTGKPAHVLLAKADKLGRQPAAERLR
                     ATTKTLAANFPGVSVQLFSAQGGIGLVEAEAVLNRWYAADK"
     gene            9923..10531
                     /locus_tag="VN496_15395"
     CDS             9923..10531
                     /locus_tag="VN496_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF18392.1"
                     /translation="MQRCIRATMALAYIVMTSAYAQGEPAKAPAIANQVCAACHGFDG
                     NSPLPSNPSIAGQHADYLLKQLTEFKSALRKNPIMTPIAASLSPDDMRDLANYYSSLP
                     PKQMAAKDETLVEQGRKLYRGGNASNGVAACAGCHSPNGAGIPSQFPRLASQHADYVA
                     TQLKAFRAGDRANDPNQMMRTTAIKLTDKDISALAEYISGLH"
     gene            complement(10528..>11426)
                     /locus_tag="VN496_15400"
     CDS             complement(10528..>11426)
                     /locus_tag="VN496_15400"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXF18393.1"
                     /translation="KFEGCYHGHADSLLVKAGSGALTLGQPSSAGVPPEITAHTLVLD
                     YNDSAMLESAFKLKGDTIAAVIVEPVAGNMNLVKPTREFLATLRDLCTRYGSILIFDE
                     VMTGFRVGLRGAQGLFDITPDLTTLGKVIGGGMPVGAFGGRRDIMQLIAPLGPVYQAG
                     TLSGNPVAVAAGLKTLELIQAPGFFERLTATATKLTERLQAMARDKGIVFSAQSVGGM
                     FGVYFRPAPPESYAEVMQCDKLAFNHFFHALLAQGIYLAPSAYEAGFVSDAHGDAQIE
                     ETVSAAGIAFTNIPGASASRTA"
BASE COUNT         2559 a         3123 c         3026 g         2718 t
ORIGIN      
        1 tctcccagta gcggcacctt tgttattgtg ctggactcgt cctgagtgta aatgccacca
       61 agacctacag taccgccatt ttctaccaag acttcggttg agacctgttt ggtatctatc
      121 gcaggcccgg cgcttgtcgc cgcaccaacg ctgtctttgt tgacgtttaa tttcattatg
      181 atgttgtcgt cgggcgttat ttgaggcttg accttcaggc tgagcgtagc cttgcggaaa
      241 gagacgcttg tcgctccact gctggtggcc tgctgatatg gaatttccgt tccctgttcg
      301 attgtcgctt cggcgttgtc cgccgtgatc acccgtggac ttgaaatgat cttgcccttg
      361 ccgtctgcct gcaaggcagt tatctccaga ttcagaaact tggtcatggc atcattcatt
      421 aaaatcaacg acgcctgtcc aggcagcacg ccgccaagtc ctggagccgc catgtttgaa
      481 aacaaggaat cggtgatgaa atttggagtg ttagacgtct gaccggtatg tgcccctgtg
      541 tcggctaagc cgccgccgat aagtgcccga tgatcgctcc cggccgcaga gccacgcccg
      601 gtgaggtcgt gatagcccaa ccgagtaccg atgtttcgac tgaatgtatc cgttgcctcc
      661 acgatgcgcg cctcgatcag tacttgccga atcgcgcgat cggtcttttt cagtagttca
      721 cgaacctctt caagcttggt aggagtgtct tgcacgaaaa tctggttggt tcttgcgtca
      781 acgacagcac tgccgcgttt ggacagaatt ctttggttcg gatcggttat gaccttctga
      841 aagtcctctg ccttctggta attcaactgg aaactttccg tgcgaaccgg ctccagatcg
      901 gagatttgct gtctggattc cagcgcgagt ttctccttcg tagcaagttc gtcgcgtggc
      961 gctatccaga cgacgttgcc ggtcttgcgc atgtccagac ccttggcctg caggatgata
     1021 tccaatgctt gatcccatgg cacatccttg agtctcaagg tcaggttacc gctcaccgta
     1081 tcactcgtga tgatgttcaa gctggtgaag tcagcaatca cctgcaacac cgcccgcact
     1141 tcgatgttct gaaagttcaa tgacagtttg tcaccggtgt agccgccggc cgatttaggt
     1201 ttgcctgcct cttctattac cggttttact tcgatgatga agcggttgtc ggtctggtaa
     1261 gccgattgtt cccagcggcc tttgggtgca accgttatcc gggtgttacc tcccgaccgg
     1321 ataacgtcga atgaatcgac cagggttccg aagtcgctga cgtcaaagcg ccgttccagt
     1381 gagggcggca gggcggcgtg aatgaaatcg accagtatgc tttttccttg ctggcgcaaa
     1441 tcaatccctg tcgtcgcatc ggaaagatca actatgattt gtgcttcacc atttctgccg
     1501 cgtctgaaat ctacgtcccg aacggcgtgc ccctcgccgc ccgccttggg ttcagcaaat
     1561 gatggcgctg tctcggtgga ggaggctgat gcagtaccgc cgctttgcag cgtgatgata
     1621 acggtgttgc cttctatctt ggtctcgtac ttgaccattt tggccaagtt aagaacaacg
     1681 cgcgtgcgcc cgcccgattg tcctaacctg atcgttcgca aatccccctg gttgacttcc
     1741 tgagaattct tgcccagtcc gttagtggta ccggggaaat cgaatgcgac gcgcggtgga
     1801 ttattgacgg taaacgcagc tggtgggttg gtcggcgcat ctttaaggcc aaactttatc
     1861 gtcacagcac ctggctggct ggagacgtct atggactgga taacattacc tgtagtggca
     1921 ggcgctgccg ccttgtcttg cccatagact gaggcgctca gcagcagccc cagtagcgtg
     1981 ggcacaaccg ccaagttcat cacacccgcg atgcctttca tatttccgtg ctccttaggt
     2041 cattgctcga gcaactgcag actgctagtg cgctcggtcc aggaaccgga cgatgagtcc
     2101 tggattactt ccttgagggt caccgttgat tcgctgaccg aagtgacgac accgaaattc
     2161 tggcctagat agttaccaaa tttcacccta tagagattgt tgtcaggagt cttgatcagc
     2221 gcgaagcgaa cttgtttctg ctcaagggtc cccaccatct gtaggctttc cagcgggaac
     2281 gcctcaaggg cctccttgcg ccggttcaaa tctgggcgtg gtccactacc acctgcagcc
     2341 cccgagcgcg tgtccgcaag agtctttcgg ggtctgaacg gatcgggcaa gtcaaaggcg
     2401 ttataggcga atggctcgaa ttgcttcact tccggaatcg gctcaacacg ccctcgcagt
     2461 ccctcacccg aatccttcac gaattgcttg agatccgaaa attgttcgcc gccacaaccg
     2521 gataaaacca gggccacaat cgcgaatatt gaacgaagcc gcatcatttt ttcttcgcgg
     2581 cagcagcgtc gcgtgccgct ttacgttgag ccgcaacttc actgtcgtcc agataacgaa
     2641 acgtttttgc tgtggcatcc agagtcaagc cactcttgct ggaacttaac gcaatatcat
     2701 tcaaggtaac gatgcgggac aattgcgaaa tgtcgctggc aaaagcgcca atgtcatgat
     2761 agttgcctgc aagcttgatg gtaatgggca attccgcata gaactccgac cgtgtttctg
     2821 cgggcgcagg tcggaacagt tcaaactgca ggccgcgacc taagcccgcc tggttgatgt
     2881 ccgtcagcag cgcttccatc tcagatttat ttggcagctg tttgagcatt tcaccgaaag
     2941 attcctcgat ttcggcaagt tgcttgcgat acgcctcaag gttcacggct tgttttttct
     3001 tatcaagaaa cgccgtgcgc agatcttgtt ctttcgcccg atctcgggcc aatgtctcaa
     3061 gttggttctg ccagtcaaac cagtagcccg caaataccag cagcagtaaa agaatgaaca
     3121 gcgccccgag ttttggcaac gcgggccagc tgccgatatc gcgcggatta agtcttcgca
     3181 ggtcgtcgag cgtcatgacg ttacgtcttt ggtgtggcgg gtgctgtggg agaaccggtt
     3241 gctggtcgcg gcacgttttc ctctttgggc cgcgaaatgt tgacgtttaa attaaattca
     3301 ttcagcggcg ctgcatcgac tgttaccgcc ttgatttcga tcaaagacgg gctctccagc
     3361 cacggcgatg cctcgaagtt gcgcatcagc gtagaaactc gtgcgtttga agtcgcgtat
     3421 ccgatgacat taactctttg cccggtttgc ctgatcgctt tgagatatat gccgtccgga
     3481 agctgccgca ccagctggtc gagtaatcgt acggtttccg ttcggtttgt ctgcaaagac
     3541 tcgactacac gtttacgtgc cagaagggct tgcgtctgtt cctttagttt ccggatttca
     3601 tctatctgct tatcgagcaa tacgatttcg gattccagat acttgttgcg ttcaacttgg
     3661 gtctctatgc gtccagcaaa aaaagtgtgc acagcaacga cgatggcagc ggccaagccg
     3721 accgtcatgc ccgcgagtac aaaaaactgc tgctgctggg ctttgcgtcg cgctgcgcgg
     3781 tgaggcagta agttgattgg gatcattcgt caaacctccg cagcgctagc ccacaggcta
     3841 ctaacagtgc cggcgcatca atcgtcaatt gcgcttgccg cactttcgag gctacttcca
     3901 ttccaacaaa tggatttgct atgagtgtat tgacttgcgt gcgctcagcg accacttcag
     3961 gaagcccggg tatggtcgcc gagcccccag acagaaatat gtgatgaacc tggttaaact
     4021 gcgtcgaagt gaagaagaat tgcagcgctc ttgcaatctc gagcgccaga gtatccatga
     4081 acggggcaag cacttcggag tcgtaagtgt ccggcaggcc tccattgcgc tttgccgctt
     4141 cggcctcttc tacggagagg ctgtaatggc gcatgatttc ctgggtcaat tggttaccgc
     4201 cgaagggttg ctctcgcata tacacggatt ggccattgcg cagcacattg atgttcatca
     4261 tcgatgcgcc aacatcaact accgcaatcg tttgatcgtg cccggcatcg ggcaattgtc
     4321 cctgcattaa ttcgaaggca ttttgggagg caaaagaatc tatatccatc accgtagcct
     4381 tgaggcccac cgattctgcg actgcaacgc ggtcctcgac cttttccttc cgggatgccg
     4441 cgataagcac ctcaacgtct tccgtactgt tcggtgcagg tccgagcgtc tgaaaatcaa
     4501 gattgacttc ttcgagcgcg aacgggatgt attgatttgc ctcggtctcg acttgggctt
     4561 cgagttgttc ctcagtctgt ccagccggca caattatttt cttggtgatt acggcggcag
     4621 atggcaacgc catcgccagg ttctttgctc tactgcccat gcgcttccag ccgcggcgca
     4681 aggcatcgcc cacttcctca aggttcatga tgttgccgtc cacgacggca tccttgggca
     4741 agggctcaat tgtgtagcgt tcaagccgat accggcccct gccggatccg gcaagttcga
     4801 ccattttcac tgacgaagag ctgatgtcta ccccaattag cggggccgac tttggtttca
     4861 gcaggttgcc gataaaatcc gcgtcaaacg caatttttcc tttgaacatt ggccctttag
     4921 acgcgatacg tataatctac attaaatgct agcaacaact attttgcatg taaagcgttt
     4981 ttatggaccc gggtgttgcc cgatggcaac accttatata atcacgctcc ttccggtact
     5041 tgacccttct gcggctcgtc gatgattttt tcccgttggt ggatttaccc aattattgga
     5101 tttgctgctc ttgcagtttt gcttgccctt gtagcgggtt atgccgctgc ggtggcttac
     5161 ccgaacttac cttcgcttga cgcgctcact tcgtatcaac cgaagatacc gctgcagatt
     5221 ttcagcatgg aaggggatct catcggtgaa ttcggggagg agaagagggc gttcgtgaag
     5281 attgaccaga cgcccgactt actgcgcaaa gcaatacttg cagccgagga cgagcgcttc
     5341 tatgagcatg ggggcgtcga ttacatcggc gtcatgcggg cagcgctctc taactttgca
     5401 tctgcaagcg cgcgccaagg cgccagtacg ataacgatgc aagtggctcg aaattttttc
     5461 ctgagcactg aaaaaacatt cagccggaaa tttaacgaag cgttgctcgc attcaaaatc
     5521 gagcacagcc tcacgaaaga cgaaatcctg caactgtata tcaatcagat ctatctgggg
     5581 caacgagctt acggctttgg tgcggcctca caagtttatt tcggcaagca gcttgatcaa
     5641 ttgagtttgg cggagcttgc catgcttgct ggactgccaa aggcaccatc ccgatacaac
     5701 ccagtcgtga acatgacccg cgccaagatc agacagcgct atgtgctgcg acgaatggcg
     5761 gatctcgggc acataaatga cgatcaactt aagaaggcag agtcgcagct gattgtgatc
     5821 tcgcgccagg tgcaggaatt ttccacccgt gccgaccact ttgcggaaat ggttcgccaa
     5881 gctattttcg atgcttacaa ggaggaagcc tacacgcgtg gctttcgcgt atacacaacg
     5941 ctgtcaaagc tccatcagga cgcggcctat cgcgcgctgc gccagggcgt aatggattac
     6001 gacaaacgcc agggataccg gggtgccgaa ggttatttca accttccgaa agaagaagtt
     6061 agcgaggaag cgctggaaga ttcactgcag gaggaactcg agagcgatga tatttatccc
     6121 gccatcgcgc tggaaatcga tccaaaggcc gttaaggcat atcgcaaggg aggggagacg
     6181 ctcacaatta ccggtgacgg gctcaagttt gctgggcgaa tgattggcag caaggcgcca
     6241 gcaaagactc ggctcaagcg cggatcattg attcgagttc agcgggatgc caagggtaac
     6301 tggcagatcg tacaactacc gcaagtcgaa tcggccctgg tctccgtcga taccgatact
     6361 ggagcaatca gagcccttgt cggcggcttc gatttttacc gaaataagta caaccatgtt
     6421 actcaagcgc gtcgacagcc aggctcaagc ttcaagcctt tcatttattc agcggcacta
     6481 gagaaaggat ttacgccagc tactatcatc aatgacgccc ctttgaccat agatgcaagc
     6541 gaaaccgggt ccgtcaactg gcagccgcgt aatttcgatg gcagtttcga tggaccgatt
     6601 cgtatgcgta cagcgcttac gaagtcgaag aatctggtat ccattcgcat cctgcaagcc
     6661 attaccccgc aatacgcaca ggactacatt tcgcgattcg gcttcgatcc aaagcaacat
     6721 ccggcgtatc tgacgatggc gctgggcgcg ggatcgacca cgccgctgga aatggtgatg
     6781 ggctatagcg tctttgccaa cggcggctac cgaatgaccc cctacttcat cgagcgcatc
     6841 gaggacacag ggggtaatgt gctgggtaaa gcaaacccgg tggtcgttgg taaaggcgcg
     6901 gaacgggtgc ttgatcctcg caacgcattt atcatgacta atatgatgca ggacgtaatc
     6961 cggggcggga ccggggcgcg cgcgatggca cttggcagaa tcgatctcgc aggtaaaacc
     7021 ggcaccacga atgaacagtt ggatgcttgg tttgcggggt tccaacgcca tttggcttca
     7081 gtcgtttggg ttggatacga tataccgcgc acgcttggcg accgggaaac cggtgccgtg
     7141 gttgcgttgc caatctggat gtcttacgtg ggcgtcgtat tgaaaggcca accggaggaa
     7201 ctacctattg cgcccgaagg cgtagtcgcg gtggcagtca atcccgagac cggattgcgc
     7261 gacaagcatg cggcggcgcc aatgatagag tacttttttc gtgagaatgt gcctcctgaa
     7321 caagaagccg gaccggaaat tgaaggggga gatacgccaa aacctcagga agaggtgaag
     7381 gaccagttgt actgactcgc atgcctaaag acaacggcca taacagacaa atgcgagagc
     7441 gcatagcaca tttggcagct cggctgatgg cagtcgacgg aatcgacgac tatgcattgg
     7501 caaaacggaa agcggcgcgt caggccggtg ctccagaaac cagaaatctc ccaaacaacg
     7561 atgaagttga gcacgcgttg cgcgcttacc agcaactgta tcaggctgac gagcaacgag
     7621 cgcgtttact ccacctgcga aaaagtgcgc gtcacatgat ggaattgctt gcgcgcttca
     7681 atccttatct ctctggcgcg gtactgtctg gccgtgctgg caagtattcc gaaatcgaat
     7741 tgcttttgtt cacggatagc atgaaggatg tcgaattgtt cctgctgaac cgccgcattg
     7801 tgtactcgtg tggggaacgc cgaatgcgga taagcgatga gtcgcgcagc gtacccgttt
     7861 tcagggtcgg tacggacgat gcggattttg atatcgctgt cctggaaccg cgcgatttgc
     7921 gatcgcagat tcgcaacgcg cccgagggcc gtccgctgga acgtgtccga attgactggc
     7981 tggaggcgga acttgccaag cccgatggat ttgccgcttg agccgtatca attttctttg
     8041 agccacatgg cggcatccag cgcgaagtag gacagaaccg catcggcccc ggcacgtttg
     8101 aatgaaagca aagattccat aacgcatttg cgttcgtcga tccagccgtt ctgtccagcc
     8161 gccttcagca tggcgtattc cccgcttacc tgatacacca gcgtcggtgc gccaaattcc
     8221 tcctttaccc ggcggacaat atccaaatat ggcatcccgg gcttgaccat caccatgtcc
     8281 gcgccttctg ccaggtccaa gccaacctcc cacagtgcct cgttggaatt ggctggatcg
     8341 acctgatagg tatatttgtt tgcttttccc aatgtggcgg aagatccgac cgcatcgcgg
     8401 aaagggccat agaagctgga tgcgaatttg gccgcatagg ccagaattct ggtgtggata
     8461 tgcgcggccg cgtccaaggc ttcgcgcaag gcaaagatac gtccatccat catgtccgac
     8521 ggtgcgacga tgtccactcc cgcttcggca tgggagagcg cttgctttac caagacctgg
     8581 atggtttcat cattgagaac atagccaccg gcatcgacca agccgtcttg tccgtgactc
     8641 gtataaggat ccagggcgat gtcagtaatg acacccaact caggaaattc tttctttagt
     8701 gcgcgaatgg ctttcggcgc caatccttcc ggattgaacg cctcctttgc atcagtggtt
     8761 ctgagccgat ggtccaccac cgggaacaaa gcaatggcag gaataccaaa ccggacacac
     8821 tcttcagccc gacgcagcag ccggtctgtg gacattcgtt ccacgccagg catcgaagga
     8881 acggcttcag ttttattttg cccatcgacc acgaacacgg gatagatcag atcatcggcc
     8941 gaaaggcggt gttcccgcac caagcggcga gaaaaatcat cgtgacgaag ccttcgcatc
     9001 ctacgccgcg ggaatcttcc gagaacctgc atgtaaatat tcctgtctgc cgtttgaggc
     9061 tagtagctta ttgattttat tccttaaaac tgtctggaac ttttcatggg gcacggtact
     9121 cttacaccgt agacagcaat gtctcccgct tctcctccct gagcgggtgc cttaatccaa
     9181 ttgaggcttt gcttgacccc ccggttttag tcccctttaa ccgggggttt tttattgtac
     9241 tgactactta tctgcggcgt accagcggtt caatactgct tcagcctcaa ccagcccaat
     9301 gccgccttgt gctgaaaata attgaaccga taccccgggg aaatttgccg cgagcgtctt
     9361 cgtggtagca cgcagtcgtt ccgcggcggg ttgccgtccc agcttgtcgg ctttggcaag
     9421 cagaacatgc gccggtttcc ccgtgcttcc ataccaggat aataattggt ggtcgatttc
     9481 cttcaaggga tgacgaatat ccatcacaac gataagaccc cgcaactgag ggcgcagcag
     9541 tagatagctg gatatgaatt gctcccagtc ctgctttatc ttttgcggaa ccttggcgta
     9601 gccatagccc ggcaaatcca ccagatagcg ttcgtttccc aaactaaaga aattgatttg
     9661 ctgcgttcgt ccaggcgtct tgctcacaaa cgccaactta tggcggcggg caagtgcgtt
     9721 gatcgcgctc gatttcccgg cattggatcg tccggcaaaa gcaacctcca tttttgaagg
     9781 cggcaagccg gtttcatcgg cagccgaacc gaagaattca atatcccgaa acagccctga
     9841 ctgactcatg agttacgata aaatgcatat tttttttggg gccgttgttg ttcgcggcgt
     9901 cgcgatatta gggagagcat gaatgcaacg ttgtatcagg gcaacaatgg cgctggcata
     9961 cattgtaatg acatccgcct acgctcaagg cgagccagcg aaagcaccag ctattgctaa
    10021 ccaggtttgt gctgcatgtc acgggttcga cggaaacagc ccgcttcctt caaatccgag
    10081 cattgcgggt cagcatgcgg actacttgct gaagcagctg actgaattca agtcggcgtt
    10141 gcgcaagaat ccgatcatga cgcctatagc ggcaagcctt tcccccgatg acatgcgtga
    10201 tttggccaac tactacagtt ctctgccgcc gaaacagatg gcagccaaag atgaaacctt
    10261 ggttgaacaa ggacgcaagc tttaccgcgg cggcaatgcg tcgaatggag ttgccgcatg
    10321 tgccggctgt cattccccta atggagcagg aataccctct cagtttccgc gtctcgcgag
    10381 ccagcatgcc gactatgttg ccacgcaatt gaaggcattt cgcgcgggag atcgcgccaa
    10441 cgatcctaac cagatgatgc gcaccaccgc aatcaaacta actgacaagg atatttccgc
    10501 gctagcagaa tacatctctg gattgcacta agccgttcgc gaggctgacg ctccggggat
    10561 gtttgtgaac gcgattccgg ctgcggacac ggtctcctca atttgggcat cgccgtgcgc
    10621 atctgacacg aatcccgctt cataagccga aggcgccaag tagattccct gcgccaaaag
    10681 cgcgtgaaaa aagtgattga aagccaactt atcacactgc attacctctg cgtaactctc
    10741 cgggggggcc ggccgaaagt aaacaccgaa cattcccccg actgattgag cggaaaatac
    10801 aattccctta tctcgcgcca tggcctgcag tcgttctgtg agtttggtcg cggttgcggt
    10861 caggcgctca aaaaaacccg gggcctggat cagctcaagc gtttttaggc cggccgcgac
    10921 ggcaacggga ttgccggaaa gagtgccggc ctgataaacc ggcccaagcg gtgcaatcaa
    10981 ctgcatgata tcccgtcggc ctccaaatgc acccaccggc atgccaccgc cgattacttt
    11041 gccaagcgta gtcagatcag gcgtaatgtc gaatagtccc tgcgcacccc gcagccccac
    11101 acggaagcca gtcattactt cgtcgaaaat caagatcgat ccgtaacgcg tgcacaaatc
    11161 acgcaatgtg gctaaaaatt cgcgggtagg cttaaccaag ttcatattgc ctgcaaccgg
    11221 ttccactatg actgcagcga tagtgtcgcc cttcagctta aaagccgatt ccagcatcgc
    11281 ggaatcgtta tagtcgagaa ccagcgtgtg cgccgtgatc tcgggcggca cgcctgcaga
    11341 acttggttgg ccaagcgtca aagcgccaga accggctttc accagcagtg aatcggcatg
    11401 cccgtggtaa cagccctcga atttga
//
LOCUS       DATMZQ010000482         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5057760, whole genome shotgun sequence.
ACCESSION   DATMZQ010000482 DATMZQ010000000
VERSION     DATMZQ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5057760"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1266)
                     /locus_tag="VN496_15405"
     CDS             complement(172..1266)
                     /locus_tag="VN496_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF18394.1"
                     /translation="MHAPPRILVVDDNAANVDILRMRLEAEGYEIVTAADGEAALTAV
                     KLHRPDLLLLDVMMPKLDGVEVCRRLRADKTLPFMPIVLVTAKSESVDVVAALDAGGD
                     EYLTKPVDKAALIARVKSMLRIKALHDSVEDWGRTLEKRVAEQVAQLERFSRLKRFFS
                     PQLAEQILAGGAEDPLKSHRREVVVVFLDLRGFTAFAETSEPEEIMGVLREYHAEMGS
                     IILAYEGTLERFAGDGMMVFFNDPVPVPDAAARAVRMSVAMHDRLAPVTAKWRKRGFD
                     LSLGVGIAQGFATIGAIGFEGRWDYGAIGTVTNLAARLCGEAKPGQTLVSSRLLASVE
                     DLARIEPLGELQLKGFLKPVPAFNVIGLAG"
     gene            complement(1272..1640)
                     /locus_tag="VN496_15410"
     CDS             complement(1272..1640)
                     /locus_tag="VN496_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF18395.1"
                     /translation="MKKRVLVIEDHEDNRRIVRDLITASGYDLLEASTGEEGIELATK
                     QRPDIILMDIQLPGMDGYEVTRRIRGDAKLKNIPIIAVTSYALSGDDQKAFAAGCDGY
                     VTKPFSPRLLLAKIREFLPD"
     gene            complement(1669..>2473)
                     /locus_tag="VN496_15415"
     CDS             complement(1669..>2473)
                     /locus_tag="VN496_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXF18396.1"
                     /translation="LLQTFAAQSVLAIQNARLFSEIDEKSRQLQVASQHKSQFLANMS
                     HELRTPLNAILGYTELIVDGIYGNVPEKIQEVLDRVQKSGRHLLGLINDVLDLSRIEA
                     GELSLTLSDYSFQDAVQSVVSAVESLASEKGLRLSVDVAAKLPVGRADERRIVQVLLN
                     LVGNAIKFTEEGEISIGVRRDDEVFIVSVSDTGPGIPANQHERIFEEFQQVDASSTRA
                     KGGSGLGLAISKKIVELHGGRIWVESVIGQGSTFTFSIPVQTLRQKLSA"
BASE COUNT          517 a          757 c          670 g          529 t
ORIGIN      
        1 gtcgatacca tcatgacaag caactcgcaa gccggcaagc ccaaacgcta ctatggtacg
       61 tgaataatat ggtagctgaa taaatcagtc tcacgccaac gaagtggtcg cgcctcttgc
      121 cgctctaagg ccagtgaaat tggacgcgca attccaaccg ggcaaaataa attaacccgc
      181 aagtccgatg acgttgaatg ccgggactgg ctttaaaaaa cccttgagtt gcaattcacc
      241 cagtggctcg attctggcta ggtcctccac cgaggccagc aagcgtgaag aaaccagggt
      301 ctgcccgggc ttcgcttccc cacacaggcg agctgccagg tttgtcacgg tgccgatcgc
      361 cccgtaatcc caccgaccct cgaagccgat tgcgccaatc gtcgcgaatc cctgcgcgat
      421 gcccacgccc aggctgaggt cgaagccgcg tttgcgccat tttgcggtga ccggcgcgag
      481 ccggtcgtgc atcgccacgc tcatgcggac tgcgcgcgct gcggcatccg gaacgggtac
      541 gggatcgtta aagaaaacca tcatgccgtc gccggcaaac cgctccagcg tgccttcata
      601 agccaggatg atactgccca tttcggcgtg gtactcgcgc agcacgccca tgatttcttc
      661 aggttccgaa gtctcggcaa aagcggtaaa tccgcgcaga tccagaaaca cgacaacgac
      721 ttcgcgccga tggctcttga gcgggtcctc ggcgcctccg gccaggatct gctctgcgag
      781 ctgcggcgaa aaaaagcgtt tgagccggct gaatcgttcc agttgcgcga cctgctccgc
      841 cacgcgcttt tccagcgtac gcccccagtc ttccacggaa tcgtgcagtg ccttgatacg
      901 cagcatcgat ttgacgcgcg cgatcagggc ggccttgtcc accggtttgg tcagatactc
      961 atcgccccct gcgtcaagag cagcgaccac atcgacggat tctgacttgg ctgtaaccag
     1021 aacaatgggc atgaacggca gcgtcttgtc ggcgcgcaga cggcggcaca cttccacacc
     1081 gtcgagcttg ggcatcatca cgtcgaggag caacagatcg ggccggtgca gtttcacagc
     1141 tgtcaacgcc gcttcaccat cggccgcggt cacgatttcg taaccctcgg cttcgagccg
     1201 catgcgcagg atgtccacat tggccgcatt atcatcaacg accagtatgc ggggcggtgc
     1261 gtgcatggca atcagtcggg aagaaactcg cggatcttcg caagcagcag gcgcgggctg
     1321 aacggcttgg tcacataacc gtcgcagccg gcggcaaaag ctttctggtc gtcgccgctc
     1381 agggcgtaag aagttaccgc aataatcggt atgtttttta gcttggcatc acccctgatc
     1441 cggcgtgtga cttcgtagcc atccatgccg ggcagctgga tatccatcag gataatgtcc
     1501 ggcctttgtt tcgtggccag ttcgatgccc tcttccccgg tcgaggcttc gagcagatcg
     1561 tatccgctgg cagtaatcag atcacggacg atgcggcggt tgtcttcgtg gtcctcgatg
     1621 accaaaactc gctttttcat tgcggtttcc tctcgttgcc agaagcactc atgcactgag
     1681 tttctgtctt agcgtctgta ccggaatgct gaacgtgaaa gtcgatcctt gtccaatgac
     1741 cgactcgacc cagattcgcc caccatgcaa ctcaactatt tttttggaaa tggcgagccc
     1801 gagcccgctg cctcccttgg cgcgcgtcga agatgcgtcg acctgctgga attcctcgaa
     1861 aatcctttca tgctgattgg caggaatgcc cggcccggtg tcggatacgg acacgatgaa
     1921 gacctcatcg tcgcggcgca cgccaatgga gatctccccc tcttcggtga atttgattgc
     1981 gttgccgacc agattcaaca atacctgcac gatgcgccgc tcgtccgccc ttcccaccgg
     2041 gagtttcgcg gcaacatcca cgctcaagcg cagtcctttt tcgcttgcca gcgattccac
     2101 agcggacacc acggactgca ccgcgtcctg gaaggaataa tccgatagcg tcagcgacag
     2161 ttcgcccgct tcaatcctgg aaagatcaag cacgtcgttg ataagaccaa gcaaatgccg
     2221 tccgcttttt tgcacgcggt cgagcacttc ctggatcttc tcgggaacat tgccgtagat
     2281 cccatcgacg atgagttcgg tatagcccag gatcgcgttg agcggcgtgc gcaactcatg
     2341 gctcatgttg gccaggaact gcgacttatg ctggctcgca acttggagct gccggctttt
     2401 ctcgtcgatc tcgctgaata accgagcatt ttggatcgcc aataccgatt gtgcggcgaa
     2461 ggtctgtaac aga
//
LOCUS       DATMZQ010000483         2555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3735669, whole genome shotgun sequence.
ACCESSION   DATMZQ010000483 DATMZQ010000000
VERSION     DATMZQ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3735669"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VN496_15420"
     CDS             <1..607
                     /locus_tag="VN496_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HXF18397.1"
                     /translation="YSKSEIIQVALFGFVFLGDRTNWIVAGAIALGTAGVLMLAPADP
                     KHPVRALLSGWTSRIGMLGIASGAGFALSAVGFRGAALALHDAPFLMAAASTLMIAQV
                     LQTLLLGGWLWLRKRDVVMRVLRAWRASLFAGFMGAAASAGWFTAMAIEPVAHVRTLG
                     LIELLFSYVVSLRLFREHLQRRELAGIGLLTLALIVVTLSH"
     gene            649..957
                     /locus_tag="VN496_15425"
     CDS             649..957
                     /locus_tag="VN496_15425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXF18398.1"
                     /translation="MVHVIATVQLAPGTRDKFLAEFRKIIPDVRAEAGCIEYGPSIDA
                     DTDIPIQYKVGADRVVVIEKWETIAALKAHGVAPHMQAYRTKVKDYVKGMELRVLSPA
                     "
     gene            complement(987..2198)
                     /gene="mmsB"
                     /locus_tag="VN496_15430"
     CDS             complement(987..2198)
                     /gene="mmsB"
                     /locus_tag="VN496_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HXF18399.1"
                     /translation="MREQVAAVGELTSQPRPGAAAGTGWLRGHLREYGLLLSLVAIMA
                     FFQVMTDGTLMQPLNLTNLVLQNSYIVIMALGMLLVIVAGHIDLSVGSVSGFIGALAA
                     VLMVSWGWHYVPATLLCLATGAAIGAAQGMWVAFFRIPSFIVTLAGMLVFKGLALALL
                     QGQSVGPFPEMFQRLSSGFIADPLGGDSLRLTSLLLGVAAAVIMVVLKVRGRAAQARH
                     GMQTEPLMVFALTQSVFAAAIVYFAWLLSSYKGLPNVLVVMVVLILGYDFITRRSTIG
                     RRIYALGGNEKAARLSGINTERLSFYTFVNMGVLAALAGLVFAARLNTATPKAGLGFE
                     LDVIAACFIGGASASGGVGKVLGAVIGAFVMGVMNNGMSILGVGIDYQQVIKGLVLLA
                     AVVVDVYNKNK"
     gene            complement(2218..>2555)
                     /locus_tag="VN496_15435"
     CDS             complement(2218..>2555)
                     /locus_tag="VN496_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXF18400.1"
                     /translation="DFVHTLVKNLSGGNQQKVVIGKWLLRGPRLFILDEPTRGIDVGA
                     KYEIYTLINSLADQGKCIIMISSEMPELLGMCDRIAVMNNGHFAAEFARGEATQEKIM
                     QAIVRDQLN"
BASE COUNT          545 a          777 c          749 g          484 t
ORIGIN      
        1 ctactccaaa agcgaaatta tccaggttgc gttgttcggc ttcgtgttcc ttggcgaccg
       61 gaccaactgg attgttgcgg gcgccatcgc gctcggaacg gccggtgtgt tgatgctggc
      121 cccggccgac cccaaacacc cagtgcgcgc gttattgagt ggctggactt cgcgcattgg
      181 catgttgggc attgcatccg gcgccggatt tgcgctgtct gccgtaggct ttcgcggtgc
      241 ggcgctcgcc ctgcacgacg ccccgttcct gatggcggcg gcgagcacgc tgatgatcgc
      301 gcaggtattg caaaccttgt tgctgggcgg ctggctctgg ttgcgcaagc gcgatgtggt
      361 catgcgagta ctgcgtgcgt ggcgggcttc actattcgcc ggcttcatgg gcgctgcggc
      421 atcggcagga tggttcaccg ccatggcaat cgaaccggtg gcgcatgttc gcacgctagg
      481 acttatcgag ttgctgttca gttatgttgt gtcactgcgc ctgtttcgcg aacatctaca
      541 gcgaagggaa ctggccggca tcgggttgct aacgctcgcc ctgatcgttg tcacgctgag
      601 tcactgatta cccagttagc ttatgtttct caaccaatag gaaaaaccat ggtccacgtc
      661 atcgcaaccg tacagctggc gccaggcacg cgcgacaagt ttctcgcgga atttcgcaag
      721 atcatccctg atgtgagagc agaagcgggt tgcattgaat atggtccgag catcgatgcc
      781 gacaccgaca tcccgatcca gtataaggtc ggcgccgacc gggtcgtagt catcgagaag
      841 tgggagacca ttgcagcgtt gaaggcacac ggcgttgcgc cgcacatgca ggcctaccgc
      901 accaaggtca aggactacgt caaaggcatg gaattgaggg tgctgtcgcc ggcctgagca
      961 cgcaagaaac ttggtgccag agcgctttac ttgtttttgt tgtagacatc cacgacaacg
     1021 gccgccagca ggaccagacc cttgatgacc tgctggtaat cgatgccaac accgaggatc
     1081 gacatgccgt tgttcatgac gcccatgacg aaggcgccaa tcacggcgcc caacaccttg
     1141 ccgacgccgc cggaagccga tgcgccaccg atgaaacagg cagcgatgac atcgagttca
     1201 aatcctaatc ccgctttagg cgtagcggtg ttcagacgcg ccgcgaaaac caaaccagcc
     1261 agcgcggcga gcacgcccat gttgacgaag gtatagaacg acagccgttc ggtattgatt
     1321 ccggacaagc gagccgcttt ttcattgccg ccgagcgcgt aaatgcgccg tccgatggta
     1381 gaacgccgcg tgatgaaatc gtaaccgagg atcagcacga ccatcacgac cagcacgttt
     1441 ggcaagccct tgtacgacga cagcagccac gcaaaataaa cgatcgccgc agcgaatacc
     1501 gattgtgtca gcgcaaacac catcagtggc tcagtctgca tgccatgcct tgcctgcgcc
     1561 gcgcgcccac gcactttcaa gaccaccatg atgactgctg cggcaacgcc cagcagcagc
     1621 gaagtcagcc gcaggctgtc acccccgagc gggtcagcaa taaatccgga gctcaggcgc
     1681 tggaacattt ccggaaacgg gccgacggac tggccttgca gcagggcgag cgcgagcccc
     1741 ttgaatacca gcatgcctgc cagcgtgaca atgaacgagg gaatacgaaa gaatgccacc
     1801 cacatgccct gcgctgcgcc gatcgccgca cccgtggcaa ggcacagcag cgtcgccggc
     1861 acataatgcc aaccccagct caccatcaaa actgcggcca gtgcgccgat gaagccgctg
     1921 accgaaccga ctgacaggtc gatgtgccct gcgacgatga cgagcagcat gcccaacgcc
     1981 atgatgacga tataactgtt ctgcagcacg aggttggtca ggttcagcgg ctgcatcaaa
     2041 gtgccgtccg tcatgacctg aaagaaggcc atgatggcga ccagcgacag caacagtccg
     2101 tattcgcgca agtggccgcg cagccagccg gtaccggcag cggcgccggg gcgcggttgc
     2161 gaggtgagtt cccctacggc agcaacttgt tctcgcatgg cgtctcgctc taatgcgtca
     2221 gttcaactgg tcccgcacga tcgcctgcat gatcttctcc tgcgtggctt cgccgcgcgc
     2281 gaactccgcg gcaaagtgcc cattgttcat gacagcgatg cgatcgcaca tgccgagcaa
     2341 ttcaggcatc tccgaggaaa tcatgataat gcacttgccc tggtccgcaa ggctgttgat
     2401 cagcgtgtaa atctcgtact tggcgccgac atcgatgccg cgcgtcggct cgtcgaggat
     2461 gaacaggcgc gggccgcgca gcagccactt gccgatgacg accttctgct gattgccgcc
     2521 ggacaggttc ttgaccaggg tatgcacaaa gtcgc
//
LOCUS       DATMZQ010000484         9462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_975835, whole genome shotgun sequence.
ACCESSION   DATMZQ010000484 DATMZQ010000000
VERSION     DATMZQ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975835"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VN496_15440"
     CDS             <1..421
                     /locus_tag="VN496_15440"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HXF18401.1"
                     /translation="RTIRVNPNNGVFTTVSRGVSYANSFIDSGSNALYFSNASIPVCS
                     TIQFQDFFCPTATQTLSATLQGTSGSASVNFQVANAEHLFLANQTFFAFSTLAGPAGP
                     ISDGIFDWGLPFFFGRKVFIAIEGQGTPAGVGPYVAF"
     gene            534..1112
                     /locus_tag="VN496_15445"
     CDS             534..1112
                     /locus_tag="VN496_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18402.1"
                     /translation="MKKLLIVLLLAVLAPANGLAHGGGWNGHYHYGGYHGAYYGGAGF
                     FLFGAALGAALTYSYSQARANPDPYYYYSAAPPVYVQSQRSYIVTPVDQPAPSTQQQY
                     NGVTELGPVAKAPDLNSSSSPLPADKWFVYPSKGQSQQQAANDRHECGRWATGQSGYD
                     PDLRVHRNTQTGPADYGRALSACLEGRGYTVR"
     gene            complement(1116..2045)
                     /locus_tag="VN496_15450"
     CDS             complement(1116..2045)
                     /locus_tag="VN496_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXF18403.1"
                     /translation="MNLRSLFKLSKAAVANWQSDFAPSMGAALAYYTVFSIAPLLIIV
                     IAVAAVVFGQDAAHAAIIEQARGMLGDNAANAIDGMLRSAQKPEQGWLASVLGIVAMV
                     VGATTVFAELESNLNRIWKTQADKGTGLWHFLHTRLLSFGLVLAIGFLLITSLAVSAA
                     IGIWARYWRFGAMESLLHAANFIVTVAVITVLFALIYRFMPRVKLHWRDVWMGALITA
                     LLFSLGKILIELYISNSGIVSSYGAAGAPVVLLLWVYYSAQIFLLGAEFTKVYAESHG
                     SRQNVKPETAASAGIHAGAVNAPRDRSGRIPTA"
     gene            2182..2436
                     /locus_tag="VN496_15455"
     CDS             2182..2436
                     /locus_tag="VN496_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18404.1"
                     /translation="MPTVLDKTLKRELTIEGKPFVVAISPQGFKIVPKGKRKGQEILW
                     SAVVNGDAALSTALNASLRHGPDVADNEAAMGGDGKLAHR"
     gene            complement(2447..3517)
                     /locus_tag="VN496_15460"
     CDS             complement(2447..3517)
                     /locus_tag="VN496_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXF18405.1"
                     /translation="MFGWDSRAAKIAWTWGLIALGFYAAFAARKALLTFVLAVFFAYM
                     MAPLVAFLERRSRRRIPHTAAVVAAFALVITGVGVGATLITPNVVEEAQNLLDQLPRK
                     LAQKTDLAARIPLPAWLEPLRNRIDAFIQDNIREATAAALPFAKSAGTQFIRFFSNLI
                     YVVLIPILAFFFVKDGRKISATLVAWLQPLAPLHELYSVFSDIHVSLSRYVSALGLLC
                     LATLLAYGSFLTAIGVPYALLLAAVAAILEFIPLVGPLMAAIVALVVSGIDGYDHLLW
                     IAAFVIVYRLFQDYILYPYLMGGSGQIHPVLVIFGLLAGEQLGGVAGIFLSVPVISVL
                     VILGRHLQDQAAADARKVPPKT"
     gene            complement(3579..5504)
                     /locus_tag="VN496_15465"
     CDS             complement(3579..5504)
                     /locus_tag="VN496_15465"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXF18406.1"
                     /translation="MTTKRRLLIAIPVVLIAGIVVAALVWDWNWFRPMLEHRASTALG
                     RKVTLQNFDIKIGWHPLLIADGIAVANPPDFPADTQFGSIETLRVRIDLGALLHRQIH
                     LLEINAERPRGDLRPGPNGEPNWKFNFGSKGGVGTPLEIGSVNISDGNIKFFDPQYKT
                     DLTLMVHTEPPKNGQDSTLLIDAKGTYAKQPITARFIGGSVLSLREPGHPYPVNLEAQ
                     NGPTVITLKGTIERPLQLAGADVDLFLRGKNLAALYPLTGVPLPPSGPYTLKGKLDYT
                     DERIRFLNFAGVVGKSDLGGNLELDLKREKRLVTAVLHSKSVDMADLAGFTGAKPRKP
                     TTNDHWRVLPDKPINVPRIRAADLDIHYKVAHFKNKKAPVDDLVAHIIVQNGFMNISP
                     LSFGVAQGRIVANVKLDGRRDVLHSVADVDFRQVDLGRLLRDSKMAKGSGLIGGTARI
                     ESTGNSTAKLLANGDGELRLFMSEGDLSALLVDLAGLEFGNALMSALGLPSRTEVRCA
                     VSDFQLQQGVLKPKILVVDTEAANVLGEGLINLQNETVDFRLRTEPKHPGILSLHAPV
                     QVRGQLKKPEIKPEKKKIVTRGAAAVALGVLVTPLAALLPTIELGLGKDNNCAAAIGA
                     MKAGAPAKDIPLAKAPQ"
     gene            complement(5669..6562)
                     /locus_tag="VN496_15470"
     CDS             complement(5669..6562)
                     /locus_tag="VN496_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXF18407.1"
                     /translation="MKFKALFQLSKAAFTSWQSDYAPSMGAALSYYTVFSIAPLLVIV
                     IAVAGLVFGQEAAHADIMEQAGGLLGENGAKAIEGMLASAQKPKEGTLASLLGILAMI
                     VGATTVFAELESNLNRIWKVETQRVSGLWHFIRTRLLSIGMVLAIGFLLIVSLVVSSA
                     IAVWGKYWSGWFEGMELLLHIVNFVVSVGVITVLFALIYRFLPRIAIGWGDVWIGALV
                     TSLLFSFGKFLIGLYVGKSGMESSYGAAGALVVLLVWVYYSSQIFLLGAEFTKVYAQH
                     HGSQKKSSQPATAGSSGPAPA"
     gene            complement(6696..6992)
                     /locus_tag="VN496_15475"
     CDS             complement(6696..6992)
                     /locus_tag="VN496_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18408.1"
                     /translation="MVLRSPKGLAQAAVNALTEAESARCARLGEIAQQRHALAVRIQA
                     GIAGIVGSDRLETNDLVVHRRSLAQLEETLEAEFEDLSKVMLHKPHEAGKKTQQ"
     gene            complement(7290..7577)
                     /locus_tag="VN496_15480"
     CDS             complement(7290..7577)
                     /locus_tag="VN496_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXF18409.1"
                     /translation="MSAYVIVDIEVHDTAAYDEYRKLVGATLTRYGGKFIVRGGTIDV
                     LEGDWKPKRIVILEFETAARAREWYDSEEYRVPKQLRMSAATGNIIAVEGI"
     gene            7755..8699
                     /locus_tag="VN496_15485"
     CDS             7755..8699
                     /locus_tag="VN496_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXF18410.1"
                     /translation="MKYKDYYEILGVKRDASNDDIRKAYRKLAHKYHPDVSKDPKGEE
                     QFKAVAEAYQTLKDPEKRAAYDNLGKHRPGEEFRPPPEWGSQFGEGNFSFDDVDLADL
                     FAGLGGGRFRTSGRRSRTAAGEDYEVTAPITLEDAYRGTELDLNLSVPDYDEQGRMRR
                     VPLSFKARIPKGVTDGERLRLPGKGGKGANGGPNGNLYLTVQLQPHALFRAAGHDLYL
                     DLPLAPWEAALGASVDVPTLGGAVRMKVPAGTNTGQKLRLAKRGLPKRREGEGDLFAI
                     VQIVVPPSLSEREKALFKDLADTSGFNPRASFPAGAAK"
     gene            8696..9028
                     /locus_tag="VN496_15490"
     CDS             8696..9028
                     /locus_tag="VN496_15490"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HXF18411.1"
                     /translation="MTIESTQAQWLHEQTEISIEELAELSGMSSELLRELVDFGALMP
                     TSLQSGQWTFTPDCIVSVRTAGRLQSDFDLDTNSVSVALGLIERIRTLEAQLRELQSQ
                     FPRLRRIG"
     gene            9166..>9462
                     /locus_tag="VN496_15495"
     CDS             9166..>9462
                     /locus_tag="VN496_15495"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HXF18412.1"
                     /translation="MKNIFITAVCAALCLASAGLIAQPVGFPAAPPKVSPAQGAIDFH
                     VHSEPDVFGRSMDDIDVAVLASRKGMRGLVLKNHVTMTADRAVLVMKIVPEIEVF"
BASE COUNT         2123 a         2747 c         2626 g         1966 t
ORIGIN      
        1 acggaccatc cgggtgaacc cgaacaacgg cgtgttcaca accgtgagcc gaggcgtttc
       61 ctatgccaac agctttatcg attcaggttc gaacgcgctt tatttctcca atgcgagcat
      121 tccggtatgc tcgacaattc agtttcagga ttttttttgc ccgaccgcga cgcagacgct
      181 ttccgccacg ctgcagggca cgagcggaag cgcctcggtg aatttccaag tcgccaatgc
      241 tgagcatctg ttcttggcca accagacttt ttttgccttc agcaccctgg ccggaccggc
      301 cggaccaatt tcagacggta tattcgactg ggggctgcca ttcttttttg gccgcaaagt
      361 attcattgcc atcgagggac aaggcacgcc ggcaggcgtc ggaccgtacg ttgcgttcta
      421 ggttgttccg gtcttgcgcc gcacggcacg cgggctcgaa cactgcgcat attggtacag
      481 ccgctcggac atgtctgcgc taaactatat ggcgtcagaa caggagaatc atcatgaaga
      541 agttactcat tgtgttgctc ttggcagtac tagcaccagc gaacgggcta gcacacggcg
      601 gcggctggaa cggccactat cactacggcg gataccacgg cgcgtattat gggggggccg
      661 gtttttttct gttcggagct gcgcttggcg ctgctttgac ttattcatac tcgcaagcca
      721 gggcgaatcc ggatccatac tactattaca gtgccgcgcc acccgtctac gtgcaaagcc
      781 agcgcagcta tatagttact ccggtagacc agcccgcccc ctcaacgcaa cagcaatata
      841 acggcgtcac ggagctgggg ccggtcgcca aagcacctga cttgaattcc agttcctcac
      901 ctcttcccgc ggacaaatgg ttcgtttatc ccagcaaagg gcaaagccag cagcaagctg
      961 cgaacgatcg ccacgagtgc ggccgctggg ctaccggcca atcgggctac gatcccgacc
     1021 tgcgcgtaca tcgcaatacg cagactggac ccgcggatta cgggcgtgct ctgagtgcct
     1081 gtcttgaagg acgcggctac accgttcgct gattgctacg cagttggaat tcttccggaa
     1141 cggtcacgcg gcgcgttgac tgcgcctgca tgtatcccgg ctgaagccgc agtctccggc
     1201 ttcacgtttt gccgcgaccc atggctttcg gcataaacct tggtaaattc cgcgcccagc
     1261 agaaaaatct gcgctgaata ataaacccag agcagcagta ctaccggtgc gcccgccgcg
     1321 ccatagctgg agacgatgcc gctgttgctg atgtagagct cgatcagtat tttgcccagc
     1381 gaaaaaagca gggccgtgat caaagcgccc atccaaacat cgcgccagtg aagtttcacg
     1441 cgcggcatga agcgataaat cagcgcaaac aggaccgtga taaccgcaac ggttacgatg
     1501 aaattcgcgg catgcagcag cgattccatc gcgccgaaac gccaatacct cgcccagatc
     1561 ccgatcgcag cactaacagc cagcgaagta atgagcagga aaccgattgc gagaaccagt
     1621 ccgaaggaca gcagtcgtgt gtgcaggaaa tgccacaagc cggtcccttt gtcggcctgc
     1681 gttttccaga ttcgattgag attggactcc agctccgcga atacggttgt agcaccgacg
     1741 accatcgcca cgatcccgag cacgctcgcc agccagccct gttccggctt ttgtgcgctc
     1801 ctcagcatgc catcgatggc gtttgcggcg ttgtccccca acatgccgcg tgcctgttcg
     1861 atgattgcgg catgcgcggc atcttgaccg aacacgacgg cggccactgc aatgacgatg
     1921 atgagaagcg gagcaatgga gaacaccgtg tagtaagcca gcgcggcacc catgctcggc
     1981 gcgaaatcag attgccagtt agcgaccgcc gccttggaca gcttgaacag ggatcgcaag
     2041 ttcatcgttt cgcttcatcg tgactgcgcg ttcattaggc aacgcctgac ggagaagttc
     2101 agggatcagt tgcggccgtg gccggaaccg acgctgttta gtccggtcta tgagcctgtg
     2161 accctacctg gagagacgac catgccaacc gtcctggata aaactctgaa acgcgaactg
     2221 accatcgagg gcaagccttt tgtggtcgcc atttcgccgc agggattcaa aatcgttcca
     2281 aaaggaaaac gcaaagggca agagattcta tggagcgccg tggtaaacgg tgatgcggca
     2341 ctatcgaccg cgctcaatgc ttcgttgaga cacgggccag acgttgcaga caacgaagcc
     2401 gcaatgggcg gtgacgggaa actcgcacac cgatgacagg attggttcag gttttcggag
     2461 gcactttcct ggcatcggca gcagcttggt cctgcaaatg cctcccgagt atcaccagaa
     2521 ccgaaataac tggcacggac agaaagatgc cggcaacgcc gcccagttgc tcgccggcca
     2581 gcaagccgaa gatgacaagt acggggtgga tctggccact cccacccatt aggtacggat
     2641 acagaatata gtcctgaaac agtcgataca cgatcacaaa ggccgcaatc catagcaggt
     2701 ggtcataacc gtcaattccc gaaaccacca gcgccacaat tgccgccatt agtggcccaa
     2761 ccaatggaat gaattccaaa atagcggcca ccgccgccag caatagcgca tagggcacac
     2821 ctattgccgt caggaaactg ccataagcca gcaaagtcgc aaggcacagc aacccgagcg
     2881 cactgacgta tcggctcagg ctcacatgaa tatctgaaaa cacgctgtag agctcgtgca
     2941 gcggagcaag tggctgcagc caggcgacca gcgtggcgct tatctttcgg ccgtccttga
     3001 cgaaaaaaaa agcaagtatc gggatcagga cgacataaat caggttgctg aaaaagcgga
     3061 tgaactgtgt gcctgcgctt ttcgcgaatg gcagcgctgc tgcggtggct tcccggatat
     3121 tgtcctggat gaatgcatca atgcggttgc gcaggggttc cagccacgct ggaagcggaa
     3181 ttctggcggc caggtcggtt ttttgcgcca gcttccgcgg aagctggtcc agcagatttt
     3241 gcgcttcttc gacgacgttc ggcgtaatca gcgttgcgcc gactccgacg cccgtaatca
     3301 ccagcgcaaa tgcggcgaca acagcggcag tatgcgggat gcgtcgtctg ctgcggcgtt
     3361 caagaaaggc caccagcgga gccatcatgt acgcaaaaaa aaccgccagc acgaaagtca
     3421 gcagagcctt gcgcgcggca aaagcagcgt aaaaccccaa ggcaataagc ccccaggtcc
     3481 aggcgatttt cgctgcgcgt gaatcccagc cgaacattcg gcatccttca tgtaagcact
     3541 gttccgatct accggaccat tccgttgatg cccgttactt attggggcgc ttttgcaagc
     3601 ggaatatcct tggccggggc gcccgctttc atcgcaccga tggcagcggc gcagttgttg
     3661 tccttgccga gcccgagctc gatcgtgggc agcagcgcgg ccagaggtgt cactaaaaca
     3721 cccagtgcga cagcggcagc accacgcgtc actatttttt tcttttccgg tttgatctcc
     3781 ggctttttca attggccgcg cacctgcacg ggagcgtgca atgacaggat gccgggatgt
     3841 ttcggttcag tgcgcaatct gaagtcgacc gtttcgttct gaagattgat caatccctct
     3901 cccagcacgt ttgccgcctc ggtatcgacc accagtattt ttggcttgag gactccctgc
     3961 tgaagttgaa aatcgcttac ggcacaacgc acttcggtgc gcgagggcag acccaaggct
     4021 gacatcaagg cgttgccgaa ctccaggccg gccaaatcga cgagcagcgc actcaaatcg
     4081 ccctcgctca tgaacaagcg caactcgcca tctccgttag ccagcaattt ggcggttgaa
     4141 tttcctgtcg attcgatgcg tgccgtcccg ccaatcagcc ccgaaccttt ggccatcttg
     4201 ctgtcgcgca acagccgacc gaggtcgacc tgccggaaat ccacatctgc aacggaatgg
     4261 agcacgtcgc gccgcccgtc cagctttaca ttggctacga tgcgcccttg cgccacgccg
     4321 aagctaagcg gggatatgtt catgaaaccg ttttgaacga tgatgtgcgc gacaagatcg
     4381 tccaccggcg cctttttgtt cttgaagtgg gcaactttgt agtggatatc aagatcggca
     4441 gcacggatgc gcggtacatt gattggctta tccggcagaa cgcgccaatg atcgttggtg
     4501 gttggcttgc gaggctttgc tccggtaaat ccagccaaat ccgccatatc tacacttttt
     4561 gaatgaagca ccgcagtgac cagtcgcttc tcgcgcttca gatcgagttc gagatttccg
     4621 ccaaggtcac ttttgccgac cacaccggcg aaattcaaaa accggatgcg ctcgtcggtg
     4681 taatcgagtt tgcctttcaa ggtatacggt ccggatggcg gcagcggcac gccggtgagc
     4741 ggatagagcg cagcgagatt tttgccgcgc aaaaataaat cgacatcagc gccggcaagt
     4801 tgcagcggcc gctcgatggt gcccttcagg gttatgacag tcgggccgtt ctgcgcctcg
     4861 agattcaccg gataaggatg tccgggttcg cggagactta acactgaacc accaataaac
     4921 cgggcggtaa tcggctgctt ggcataagtg ccttttgcat cgatcagcag agtggaatcc
     4981 tgaccgttct tcggtggctc ggtatgcacc atgagcgtga ggtcagtttt gtactgcgga
     5041 tcgaaaaatt tgatattgcc atcgctgata ttgacgctgc caatctctaa cggcgtaccg
     5101 accccccctt tgctaccgaa gttgaacttc cagttcggtt ccccgttcgg gccgggacgc
     5161 aggtcgcccc gtggacgttc ggcattaatt tccagcaaat gaatttgccg gtgcagaagg
     5221 gcaccaaggt cgatccgtac cctcagtgtt tcaatggagc caaattgagt gtcagctgga
     5281 aaatccggtg gatttgcgac ggcaatgccg tccgctatca acagcggatg ccatcctatt
     5341 tttatatcga aattctgcag cgtaaccttg cggcccagcg ctgtgctggc gcgatgctcc
     5401 agcattggcc gaaaccagtt ccagtcccaa acgagcgctg ccacgacgat gcccgcgatc
     5461 aagacgaccg ggatggcgat cagcaatcgg cgcttcgttg tcatgctttt ttcggtagtg
     5521 cgggcaggcg cgaacagcgt cttatttggc tggtgccaat tgatcgtggc catgggaccc
     5581 ggagcaacgg cttatcgttc ccgccatact ccgttcggct tggcgctgaa accgatgcga
     5641 acaatgcagc tttgcgccgt ggcggcgttt acgcaggcgc cggtcccgaa gatccagccg
     5701 tggcaggctg gctcgatttt ttttgtgacc catggtgctg cgcataaact ttggtgaact
     5761 cggcgccaag caaaaagatc tgcgaggagt aatacaccca caccaatagc actaccagcg
     5821 ccccggccgc tccatagctc gattccatgc cgctctttcc gacgtaaagc ccaatcaaga
     5881 atttgccgaa tgagaacagc agagaagtca ccaatgcgcc aatccataca tcaccccagc
     5941 caatggcaat gcggggcaag aagcggtaga tcaacgcaaa caggactgtg atgacgccta
     6001 ccgaaacaac gaagttaacg atatgcaaca gtagttccat gccttcgaac cagccgctcc
     6061 agtatttccc ccacaccgcg atcgcactgc tgaccaccaa cgagactatc aacaggaaac
     6121 caatggccaa taccatgccg atcgatagca gacgtgtgcg gatgaaatgc cacaacccgg
     6181 aaaccctctg ggtctccact ttccagatgc ggttcagatt ggactccagt tcggcgaaca
     6241 cagtagttgc accgacgatc atggcaagaa tcccgagcag gctggccaac gtgccttcct
     6301 tcggtttttg cgcgctcgca agcatgcctt caatcgcttt tgcgccattt tctccgagca
     6361 gacctccggc ctgctccatg atgtctgcat gggccgcttc ctgtccgaat acaagaccgg
     6421 ccacggcaat aacgataacg agcaagggag caatggaaaa caccgtgtaa taagacaatg
     6481 cggcccccat gctgggtgcg taatcggatt gccagctggt gaacgcggcc ttcgacaact
     6541 ggaacagcgc cttgaacttc attcttagcc gttggttaag tcgccataat ttaggcaccg
     6601 gctacccggg aagttcaggg gattggttct ttcggactct gcatcgggtt tgacgcaggc
     6661 gcacacgctg cgaaggcgcg ggctgaagac ggactctatt gctgtgtttt cttgccggct
     6721 tcgtgaggtt tgtgaagcat gactttcgaa aggtcttcga attctgcttc aagtgtctct
     6781 tccagttgcg caagactccg ccggtgcacg accagatcgt tggtttcaag gcgatcactg
     6841 cccactattc cggcaatgcc ggcctgaata cgcacagcca acgcgtggcg ttgctgcgca
     6901 atttccccca gccgcgcgca acgggctgat tcggcctcgg tcagcgcatt aacagctgcc
     6961 tgcgcaagcc ctttaggcga gcgcaggacc attccgcaat tcctgcagct tggcaaaggc
     7021 gcgtcatgac tgccgggcgt cccggccttt gtgcggaaag aaccaggcat cacatgtctc
     7081 ccccattctt gcaaatccat ttacggccga tgcatcaaaa cgggttctgc attgacgtat
     7141 cggatcggga tcacggtgtc atgaagatac gcccgctgca gtatgcagta aaggcgccgt
     7201 tgcttgttgc atgctgaaca caaagccagc gtgcgatcgg caattgacag tgccagcatc
     7261 ggccttcagc ctaccggccc ggcgcaggtt cagatgcctt cgacggcgat gatgtttccc
     7321 gtggccgcgc tcatgcgcaa ttgctttggc acccgatatt cctcagagtc ataccattcc
     7381 cgggcgcgtg ccgcagtttc aaactcaagg atcacaatgc gcttgggctt ccagtcgcct
     7441 tcgagcacat cgatagtgcc gccgcgcacg ataaacttgc cgccataccg cgtcagcgtt
     7501 gcgccgacca gtttgcgata ctcgtcatac gccgctgtat cgtggacttc aatatcgacg
     7561 atcacgtaag cagacatgct tgaacctccc cagcttagga tcagcaagaa tagaaaatct
     7621 aacgtccgaa agccacaaaa accgctcgtt gcccggggtg aattgaatgc ctggaacata
     7681 tatcttgaat ccggctcaaa gaggcgtcat cttggccatc aggcggccga ccggccgggc
     7741 atggacatca accgatgaaa tataaggact actacgaaat cctgggcgtc aagcgcgacg
     7801 ccagtaatga cgacattcgc aaggcgtacc gcaagctggc ccacaaatac catcctgacg
     7861 tttccaagga tccaaagggc gaggaacagt tcaaggccgt tgcggaggcg taccagacac
     7921 tgaaggatcc ggaaaagcgg gcagcctacg ataacctcgg caaacataga cccggtgaag
     7981 aatttcgtcc accgcccgag tggggcagcc aattcggaga aggaaatttt tcgtttgacg
     8041 atgttgacct tgccgacttg tttgccggtc tggggggcgg acgatttcgc accagcggcc
     8101 ggcgcagccg gactgcagcg ggagaggatt atgaagtcac ggcgcccatc acgctcgagg
     8161 acgcctatcg cggcacggag ctggacctga atctgagtgt tccggactat gacgagcagg
     8221 gtcgcatgcg acgcgtgccg ctctcgttca aagcgcgcat tccaaaaggg gtgaccgatg
     8281 gcgaaagatt gcgtttgccg ggcaaaggcg gcaaaggggc caacggcggt ccgaatggaa
     8341 atctgtatct cactgttcaa ttgcagccgc atgcgctgtt ccgcgccgcc ggccatgatc
     8401 tgtatctgga tctgccgctc gcgccttggg aagcagcgct tggagccagc gtagatgtcc
     8461 cgacgcttgg tggagcggtc cgcatgaaag tgccggccgg aaccaatacc gggcaaaagt
     8521 tgcggctcgc caaacgcgga ttgcccaagc gccgcgaagg tgaaggcgac ttgtttgcca
     8581 tcgtgcagat tgtcgtacca ccttcacttt ccgaacggga aaaggctctg ttcaaggatc
     8641 tggctgatac ttccggattc aatccccgcg cctcatttcc ggcaggagca gcgaaatgac
     8701 tatcgaatct acccaggcgc agtggctgca cgagcaaacg gaaatttcga tcgaggagct
     8761 ggctgaattg tccggtatgt caagtgagct gttgcgcgaa ctggtcgatt tcggcgcact
     8821 gatgccaacc agcctgcagt ctggacaatg gaccttcaca ccggactgta ttgtgtctgt
     8881 gcgcacggcc ggccggctgc aatcggattt tgatctcgac acaaattccg tgtccgttgc
     8941 gttgggactg attgaacgca tcagaaccct tgaagcgcaa ttgcgcgaat tgcagtcgca
     9001 gtttccacgc ttgcggcgaa tcgggtagga ctacgcttcg gcagcattgc ggctgccggt
     9061 aaccgcttaa tttgcttcca ccctggtctg ctgcgcgact tctggcatca tggtcgaaca
     9121 cagcgcatcc gcatcgggat gcgacaggcc gggaggacag ccgccatgaa aaatatcttc
     9181 atcactgccg tgtgcgcggc gctctgcctg gcgtccgctg ggctgatagc acaaccggtg
     9241 ggctttcccg cagcgcctcc gaaggtcagc cccgcccagg gcgcgattga ttttcacgtc
     9301 cattccgaac ccgacgtatt tggccgcagc atggatgaca tcgatgttgc ggtcctggca
     9361 tcgcgcaagg ggatgcgcgg cctggtcctg aagaatcatg tgaccatgac ggccgatcgc
     9421 gcggtgctgg tcatgaagat cgtgccggag atagaggtct tc
//
LOCUS       DATMZQ010000485         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2297221, whole genome shotgun sequence.
ACCESSION   DATMZQ010000485 DATMZQ010000000
VERSION     DATMZQ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297221"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VN496_15500"
     CDS             <1..1765
                     /locus_tag="VN496_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF18413.1"
                     /translation="FNVRNSDRSRPVSDLTHRLDYLTLTNDAREVLEKLLPIEREVRD
                     DAQRWYVVRLLPYRSLEDRIGGVVVTFVDITAQQRAIQEAVESKKHAESIVNTIKEPL
                     LVLTPDLRTHSANLAFYEEFKMKPDQVQGRLLYALGEGQFDIPQLRNLLEGVLPRNVA
                     FEDFQIVYRSQVLGERLILVNGRRLGEENLILLGFQDVTGLKTVEFALRDADRRKDEF
                     LATLAHELRNPLAAIYNALAVQKIAGLGDTVPVLTHEILERQTQQLVRLVDDLLDVAR
                     ITQGKVDLRKEQLDLVAIVRSAIAISAPAIEAAGCELHTDLALEPLLINADQVRMTQI
                     ISNLLNNATKYTPKGGTITVTLAQKQNQARLAIRDSGIGIAADFLPRIFDLFVQVDGP
                     DITREHRGLGIGLTLVRNLIELHGGDIEAKSEGFGKGSEFVIHLPLLNAASESNAVST
                     VPVKGESVPRALHVLVVDDDRDSGDSLGLLLRLQGHEVDVVYDGATALASIAANPPAL
                     ALIDIGMPSMDGYEVARRVRQAPFKNVILVALTGWGQEKDRRLSAEAGFDHHLVKPAS
                     RESLNSLFASIQSLRDKPQLK"
     gene            complement(2265..2417)
                     /locus_tag="VN496_15505"
     CDS             complement(2265..2417)
                     /locus_tag="VN496_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18414.1"
                     /translation="MLKKEQELLREGYQLVRGPVLKLREYCRIEGPYPWSAFSLVWAE
                     AAFGYS"
     gene            complement(2524..2859)
                     /locus_tag="VN496_15510"
     CDS             complement(2524..2859)
                     /locus_tag="VN496_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18415.1"
                     /translation="MPNKKILQKKIASSMLIHPTATVFAGVMSNMLQWRFEKIGGTWY
                     WTAGERTTTTGPFPSFISCLVDAQEHGLFAQGYEPRKVPVAPLAAIERRSVGKKIPEL
                     LFAPMVIGH"
BASE COUNT          698 a          836 c          879 g          686 t
ORIGIN      
        1 tttcaacgtt cgcaacagcg atcgtagccg cccggtctcg gatctgacgc atcgacttga
       61 ttatctgacg ctgaccaatg acgcccggga agtacttgag aaattgttac cgatcgagcg
      121 cgaagtccgt gatgacgcgc aacgctggta tgttgtccgg ctattgcctt accgctcact
      181 ggaagaccgg attggcggcg tagtcgttac gtttgttgat attacggcac agcagcgcgc
      241 gatacaggag gctgtcgaat cgaagaagca tgctgaaagc atcgtcaaca ccatcaagga
      301 accgctgctg gtactgaccc ccgatctgcg gacgcattcg gccaatctgg ccttctacga
      361 ggagttcaag atgaaaccgg accaggttca gggacggttg ctctatgcgt tgggcgaagg
      421 ccaattcgac atcccgcaat tgcgaaatct gcttgagggc gttttgccac gcaacgttgc
      481 atttgaagat tttcagattg tgtaccggtc tcaggtgctg ggcgaacgtt tgatcctggt
      541 caatggccgc cgtcttggcg aagaaaattt gattctgctg gggttccagg atgtgacggg
      601 actgaaaaca gtagagttcg ccctgcgcga tgcggatcgg cgcaaggacg agttccttgc
      661 gaccttggcc cacgagctgc gcaatccgct ggcggcgatc tacaacgcgc tggcggtgca
      721 gaagattgcc ggtctgggcg ataccgtccc ggtgctgacg catgaaatat tggagcgcca
      781 gacgcagcag ctcgtgcggc tggtcgatga tctgctggac gtggctcgca taacgcaggg
      841 caaggtcgat ttgcgcaagg agcaactcga tctggtagct atcgtgcgga gcgccattgc
      901 gatcagtgcg ccagcgattg aagcagccgg atgtgaatta catactgacc ttgctttgga
      961 accgttgctg atcaatgccg atcaggtgcg catgacgcaa atcatttcaa atcttctcaa
     1021 taacgccact aaatacacgc ccaaaggcgg gaccatcacc gtcacgctgg cacagaaaca
     1081 aaatcaggcc cgcctggcga ttcgtgacag cggcattggc atcgcggctg atttcctgcc
     1141 cagaattttt gatttattcg tgcaagtcga cggcccggac atcaccaggg aacatagggg
     1201 cctcggcatc gggcttacgc tggttcgcaa tctgattgaa ctgcatggcg gagacatcga
     1261 ggccaaaagc gagggtttcg gcaaaggaag cgagttcgtc atccatttgc cgctcctcaa
     1321 tgcagcaagc gagagcaatg cggtttctac ggtgccggtg aaaggggaga gcgtgccacg
     1381 ggctttgcat gtgctcgtgg tagacgacga tcgggattca ggcgacagcc ttggcttgct
     1441 gctccggtta cagggtcacg aagtggatgt tgtttatgac ggtgccacag cactcgcgtc
     1501 cattgctgcc aatccgcctg cgctcgcact gatcgatatc ggaatgccgt ccatggacgg
     1561 ttatgaagtt gcccgccggg tacgccaggc gccgtttaag aatgtcatcc tcgttgcgct
     1621 gaccggttgg ggacaggaga aggaccggcg gttgtccgcc gaggcgggtt tcgaccatca
     1681 tctggtcaag ccggcatccc gggaatcact caacagtttg ttcgcttcga ttcagtcact
     1741 ccgcgacaaa ccccaactga agtaatgcag gtcgaaccgt cctggcgaag acgcgatagc
     1801 gacgacgctg attatccaga aggcgatccc tggcctaccc agtcagactt ttcctacacg
     1861 cgtataggtg cctgccagct attaccgcgg gattgacagg ggtataacga actacgaaac
     1921 agggagaagg gcgcgtggtc gcctgcgatg aaacggtatt gcattgagcg tcccgcttac
     1981 accggcaaga tttgaataac aacacacagg ctgaattgtg aaaagtcctg cttatgcctc
     2041 taccggcggg ccgattgaga cgatggcacc cgtcgaacac tcgctcatca acaaaggcta
     2101 ccggcgcgtt gccagtgcaa accggccgga gccgggcccc atggaatatc agcggtggga
     2161 ttgggcgtcc atagaaaacg gtagaccggt aacaagagtc gtcctgcttt ggcacgactc
     2221 ttaggcctgt cgttcgattc ccggcaagcg ggcgcgaaat cggatcatga atatccgaac
     2281 gccgcttctg cccacacaag actgaacgcg gaccacgggt aagggccttc gatgcggcag
     2341 tattcccgca gctttagcac tggccctcgc accaattggt aaccttcccg caacagttcc
     2401 tgctcttttt tcagcatttg agtgattgaa ccggctgtca ggcacatcag cattgttcag
     2461 tctcctttcc ttctcaagca gattggaata ttccgcgcag cgagttgtca cgcgcgtcta
     2521 ctcttagtga ccaatgacca tcggcgcgaa cagaagctcg ggtatttttt ttcctaccga
     2581 ccggcgttcg atggcagcca gcggtgcaac cggcactttt cttggttcgt agccctgggc
     2641 gaaaagcccg tgttcctgtg catcaaccag gcacgaaata aaactgggga aaggcccagt
     2701 ggtggttgtg cgttcaccgg ccgtccaata ccaagtgccg ccgatttttt cgaaccgcca
     2761 ttgcaacatg ttgctcatga caccagcgaa aactgttgcg gtagggtgga tcagcataga
     2821 actggcgatt tttttctgca agattttttt gttcggcatg tcatgcgatc gtgtcaccaa
     2881 atcccgacag tggcgagata acatccttgt catatttcgt cgattgagct ggccgcgaaa
     2941 tttttccgtt aattgtccgg ggcctgcagc ttatgaacgg tgcctccgga tgtgaccgat
     3001 ggcgacgcaa tcccggcaga tgaatgaaca aaaccttgcc ggcacacaaa aatacggtcc
     3061 atgcctgcaa aaccagaaca tcgcgcttca acatggcag
//
LOCUS       DATMZQ010000486         6706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1606838, whole genome shotgun sequence.
ACCESSION   DATMZQ010000486 DATMZQ010000000
VERSION     DATMZQ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606838"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VN496_15515"
     CDS             <1..818
                     /locus_tag="VN496_15515"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HXF18416.1"
                     /translation="HRTAFFVSDRTGITAEMLGHSLLTQFEHVSFTEITLPFVDSREK
                     AKETARLIDLTAGKDGSRPLVFSTLVDKNLSGIIQAANVFYIDCFQVFIAPMEAELGV
                     QSSHTVGRSHSVRNNSSYFNRIEAVNFALSHDDGQTTKELDAADVILVGVSRCGKTPT
                     CLYMALQFGIRAANYPLIPEDFEAKKLPGTLEPYRPKMFGLTIRADRLQRIRHERRPA
                     STYSSMANCEYEIREAEMLMRAEGIPILDTTTKSIEELATTILQKAHLQRHVY"
     gene            complement(904..1488)
                     /gene="rnhB"
                     /locus_tag="VN496_15520"
     CDS             complement(904..1488)
                     /gene="rnhB"
                     /locus_tag="VN496_15520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXF18417.1"
                     /translation="MRRGLLICGVDEAGRGPLAGPVFAACVILHKNDPIEGLADSKVL
                     SSERREELAIEIRHRAAGWAVASASVEEIDRINILRASLLAMRRAVEQLVLEPHEVLV
                     DGLHCPEVRFPVRAIIDGDALVAEISAASILAKTARDALMRQLHALYPDYGFASHKGY
                     STPEHLEALRRFGVCPIHRRTFAPVRSLCLDFGD"
     gene            complement(1485..2699)
                     /gene="lpxB"
                     /locus_tag="VN496_15525"
     CDS             complement(1485..2699)
                     /gene="lpxB"
                     /locus_tag="VN496_15525"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXF18418.1"
                     /translation="MNNLSSRTVRIALVAGETSGDLLGAHLLAALKERMPQLEAFGIG
                     GHRMQALGMRSWYPMEWFAVRGYVEVLKSLPKLLRVRRELRRRLLADPPDLFIGIDAP
                     DFNIELERTLRAKGITTVQYVGPAIWGWRGERIHKIKRAVSKVLALFPFEPAIYEKAG
                     VPVAYVGHPLADGFPENPDRTAAREQMRISPKQTVVAVLPGSRQSEVREMGELFIATA
                     RLVARQIPNPLFLVPLVSRETRYIFEQAIYRQQAEDLPITILFGHAHMAMTAADAVLV
                     ASGTATLEAALLKRPMVISYKMPRLSAWIMRRKAHVPYAGLPNILAGEFVVPELLQED
                     ATAENLAQALCNQLEDKEVRKRLENKFMAIHRSLKQDTARLAVEAILPLLKTTQTPTL
                     VEAAAAREGMQA"
     gene            complement(2705..3475)
                     /gene="lpxA"
                     /locus_tag="VN496_15530"
     CDS             complement(2705..3475)
                     /gene="lpxA"
                     /locus_tag="VN496_15530"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXF18419.1"
                     /translation="MIHASAIIHPGAKIAQDVEIAAYAVIGEHVEIGQGSSVGSHTVI
                     TGHTRIGTLNRIYQHAVIGGEPQDKKYAGEPTRLEIGDHNVIREFCTLNTGTVQDAGV
                     TRVGDHNWIMAYVHMAHDSQVGSHTILANMVTLAGHVAIHDHAILGGGTMVHQFCRVG
                     AHAFTAGGTIVLRDVPPFVMASGNSAEPHGINAEGLKRRGFSSGAIEQIRRAYKTLYK
                     SGLTFEEAKQALQAQVADCPELAMFVEFLHSSKRSIIR"
     gene            complement(3472..3939)
                     /gene="fabZ"
                     /locus_tag="VN496_15535"
     CDS             complement(3472..3939)
                     /gene="fabZ"
                     /locus_tag="VN496_15535"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXF18420.1"
                     /translation="MTLDAMEIQEIMEHLPHRYPMLLIDRVLSVDPGKEIIALKNVTI
                     NEPFFAGHFPHYPVMPGVLIVEAMAQAAAVLAFKTFNLKSDSKSVYYFVGIDQARFKK
                     PVMPGDRLILKVQILRAIRGIWKFSAQALVEDSMVSEAQLLCTMRGNPGNAAR"
     gene            complement(3936..4982)
                     /gene="lpxD"
                     /locus_tag="VN496_15540"
     CDS             complement(3936..4982)
                     /gene="lpxD"
                     /locus_tag="VN496_15540"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXF18421.1"
                     /translation="MKGEQGRSFSLAELAGRFGGTLIGDPDVRIHQVAPLETAGPQEL
                     SFLTNRKYQKLLSTTRAGAVLLAEDYAAAARLPRIVCKNPYASYARIAALINPESRPP
                     AGIHAQAVVDARARVAATASVGPCTVIESGAEVGDEAIIGANCFVGEQVRIGAGTRLH
                     ANVSVYHKCEIGQRVILHSGVVIGADGFGMAMDEGRWLKIPQIGRVIIGDDVEIGAST
                     TIDRGALDDTVIEEGVKLDNQIQIGHNCRIGAHTAIAGCTGIAGSTTIGKYCMLGGNA
                     MISGHLTIADHVVVSGGTLVAKSINSPGTYTAVFPMQPHAQWLKNAALLRHLHDLMET
                     VRSLENEVKKMRKG"
     gene            complement(5046..5546)
                     /locus_tag="VN496_15545"
     CDS             complement(5046..5546)
                     /locus_tag="VN496_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXF18422.1"
                     /translation="MNKFPLVLAAAALLAYANFAGAAELKVGFVKLERLFKEAAPAVK
                     AQKKIEKEFASRDQELQKLAKQARDLQTSLEKDGVTMGESERRDKEQDLARMNRDLQR
                     MQRELREDLNLRKNEELAAVLERANKVIQQIAESEKFDLILQEAVYISPRIDITDRVI
                     KALADK"
     gene            complement(5580..>6706)
                     /gene="bamA"
                     /locus_tag="VN496_15550"
     CDS             complement(5580..>6706)
                     /gene="bamA"
                     /locus_tag="VN496_15550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXF18423.1"
                     /translation="NRSKQRIDKLGYFSEVQVDTPAVPGTTDQVDVEVKVTERATGNL
                     TAGIGYSTAEKVILSAGVSQSNIFGTGNALSFQVSSGSVNQIYSVSYTNPYYTDDGVS
                     RGFDVYKRRVDTANLSGIATYNTSTIGGGVRFGVPITEFDTINYGLAYEQTQIGLFAN
                     TPQRYVDFVKEFGSHINSNPATVGWARDKRDSVIYTTSGTLQRVSAEIGLPTAELTYY
                     RTTYQVQWYHPLTRDLVFYTSGQIGYAAGYQQKPLPFFKVFYLGGVNSLRGYETASVG
                     PRDTNGDALGGSHLLNGSVEVLFPFPGLQKDKSVRLSWFIDAGTLGEQYDLSETRYST
                     GLSFNWYSPVGPMKISFARAINPRPEDRRQPVQFTLGTVF"
BASE COUNT         1355 a         2033 c         1852 g         1466 t
ORIGIN      
        1 cccatcgcac cgcgttcttc gtttctgacc gcaccggcat tacggcggag atgctcgggc
       61 atagcttgtt gacccagttc gaacacgtct cgttcactga aatcacgttg cccttcgtcg
      121 attcgcggga aaaagccaaa gaaacggcgc gcctgataga tctcaccgcc ggaaaggacg
      181 gcagccggcc gctggtgttc agcacccttg tggacaaaaa cctgagcggc ataatccagg
      241 ccgcgaatgt tttctacatc gattgtttcc aggtgttcat cgctccgatg gaagccgaac
      301 ttggcgtgca atcgtcccac acggtcggac gttcgcacag cgtgcgcaat aacagttcct
      361 atttcaaccg tatcgaggcc gtcaatttcg cgctttccca cgacgatggg caaaccacca
      421 aggaactcga tgccgccgat gtcattctgg tcggcgtctc ccgctgtggc aagacgccaa
      481 cctgcctata catggcgctg cagttcggta ttcgggccgc aaactatccg ctgattccgg
      541 aagatttcga ggcgaagaag ctgcccggca ctctggagcc ttaccgtccg aaaatgtttg
      601 gcttgacgat ccgggcagac cgtttgcagc gcattcgcca cgagcggcga ccggccagca
      661 cctactcgag catggcgaat tgcgaatacg aaattcgcga agccgaaatg ttaatgcggg
      721 ccgaaggcat tccgatcctg gacacaacca ccaaatcaat cgaagaactt gccacgacca
      781 ttctgcagaa ggcccatctg cagcgtcacg tttactaaag cactaaatca aaccagtaga
      841 gattgcttgt tttgctgctc tcagccgttc aagcagaaac ggttgtgtaa agcgcaattt
      901 tttctaatcc ccgaagtcca ggcacagcga acgcaccgga gcgaatgttc gccggtgaat
      961 agggcacaca ccgaagcgcc gcaaggcctc cagatgctcc ggagtggaat agcctttatg
     1021 actggcgaat ccatagtcgg gataaagggc gtgcaattgc cgcatcaacg catcgcgtgc
     1081 cgtcttcgcc aatattgagg ccgccgatat ttccgcgacc agcgcgtcgc catcgataat
     1141 cgcgcggacc ggaaagcgca cttccggaca atgcagtcca tcaaccagca cttcatgcgg
     1201 ttctagcacc agctgctcta ctgcacgccg catggccagc agactcgcgc gcaggatatt
     1261 aatgcgatca atttcctcga ccgatgcgct tgcgactgcc cagccggctg cgcgatggcg
     1321 gatttcgatc gccagctctt cgcggcgctc gcttgacagc acctttgaat cggccagccc
     1381 ctcgatcgga tcgttcttat gcaaaatcac gcaagcagcg aaaaccggac cggcgagcgg
     1441 tccccgacca gcctcgtcca caccacagat cagcagccca cgcctcatgc ttgcatgccc
     1501 tcacgcgcgg ccgcggcttc gacaagggtc ggcgtctggg tggttttcag cagcggcaaa
     1561 atcgcttcga cggccagtct ggcggtgtcc tgtttgagcg agcgatgaat tgccatgaat
     1621 ttgttctcca gtcttttgcg cacctccttg tcttccagct ggttgcacag ggcctgggcc
     1681 aggttctccg cggtggcatc ttcctgcagc aattccggaa cgacgaattc cccggccagg
     1741 atgttcggca accccgcata cggaacatgc gccttgcgac gcatgatcca tgcggacagt
     1801 cgcggcatct tgtagctgat taccatggga cgtttcaaga gtgctgcttc tagggtcgcg
     1861 gtcccggacg cgaccagtac cgcgtcagca gccgtcatcg ccatatgcgc gtgcccaaac
     1921 aggatggtga ttggcaagtc ctcggcttgt tgccggtaga tcgcttgctc gaagatgtat
     1981 cgggtttcac gactgaccag ggggaccaga aacagcgggt tcgggatctg cctggcgacc
     2041 agtctcgccg tggcgatgaa caattccccc atttcccgga cttcgctctg gcggctgccg
     2101 ggtagcacgg ccaccacggt ctgtttcggc gaaatacgca tttgttcgcg cgctgcagtc
     2161 cggtccggat tttccggaaa tccatcggca agcggatgac ccacataggc caccggcacg
     2221 ccggctttct catagatcgc cggctcgaag ggaaataaag ccagcacctt cgacaccgcg
     2281 cgcttgatct tgtgaatgcg ctcgccacgc catccccaga tcgcgggacc gacatactgc
     2341 actgtcgtga tgcccttcgc gcgcagcgtc ctttccagct cgatattgaa atctggcgca
     2401 tcgatcccaa tgaacaaatc cgggggatcg gccagtagtc gccggcgcag ttcgcgacgc
     2461 acacgaagca actttggcag gctcttcaag acctcgacgt agccgcgcac ggcgaaccac
     2521 tccatcgggt accaggagcg catgcccagc gcctgcatgc ggtgcccgcc gataccgaaa
     2581 gcctcaagct gcggcatgcg ctctttcagc gcggccagca aatgtgctcc gagcagatcg
     2641 ccggacgttt ctcccgccac caatgcgatg cggactgtcc gggacgacag gttattcatc
     2701 tgggctagcg gataatgctg cgcttcgagg agtggaggaa ctccacgaac atcgccagtt
     2761 cagggcaatc cgcaacctgc gcttgcaaag cctgcttggc ttcttcgaac gtcagcccgg
     2821 atttgtacag cgttttataa gcgcggcgga tttgctcgat ggcccccgag ctgaaaccgc
     2881 ggcgcttcaa gccttccgca tttatgccgt gcggctccgc cgaattccca cttgccatga
     2941 cgaacggcgg gacgtcgcgc agcacgattg tgccgcccgc cgtgaaggca tgggcgccga
     3001 cccggcaaaa ctggtggacc atggtcccgc cgcccagaat ggcgtggtca tggatggcaa
     3061 cgtggcctgc cagtgtcacc atgttcgcca ggatagtatg gctgccgacc tgcgaatcat
     3121 gcgccatgtg gacataggcc atgatccagt tgtgatcgcc gacgcgcgtt acgccggcat
     3181 cctgaaccgt tccggtattc agcgtgcaga attcgcggat gacattgtga tcgccaattt
     3241 ccaggcgcgt aggttcgccc gcatatttct tgtcctgggg ttcaccgcca atgacggcat
     3301 gctgatagat ccggttgagc gttccgatgc gggtatgccc ggtgattacg gtatgcgacc
     3361 caaccgagct gccctggccg atctcgacat gctccccgat aaccgcatac gcagcgatct
     3421 cgacgtcctg ggcgattttg gcgccgggat ggatgattgc tgacgcatgg atcatcgagc
     3481 agcgtttccg ggattaccgc gcatcgtgca cagcagttgt gcttccgaaa ccatgctgtc
     3541 ctcaaccaat gcctgtgcgg agaacttcca gatgccgcgt atcgcgcgca gtatctgtac
     3601 tttcagtatc agccggtctc caggcatcac cggcttcttg aaccgggctt gatcgatgcc
     3661 gacaaagtag tacacgcttt tgctatcgct tttcaggttg aaggttttga aggccagcac
     3721 tgctgccgcc tgcgccattg cttcgacgat cagaacgccc ggcataaccg ggtaatgtgg
     3781 aaaatgcccg gcaaaaaaag gttcgttgat ggtcacattc ttcagcgcga tgatttcctt
     3841 gcccggatcg accgacagca cgcggtcaat cagaagcatc ggataccgat gcggcaggtg
     3901 ttccatgatt tcctgaatct ccatcgcatc caaagtcatc cctttctcat tttttttact
     3961 tcgttttcga ggctgcggac cgtttccatc agatcatgca ggtgccgcaa cagcgcggca
     4021 ttcttaagcc attgtgcgtg cggttgcatt ggaaatactg ccgtgtaggt gcccggtgag
     4081 ttgatggatt tggccaccag cgtaccgccg gaaaccacca catggtcggc aattgtcaga
     4141 tgcccggaaa tcatcgcatt gccgcccagc atgcagtatt tgcctatcgt tgtgctgccg
     4201 gcgatccctg tgcatccggc tatcgcggta tgtgcaccga tacggcagtt gtggccgatc
     4261 tggatctgat tgtccagttt cacgccttct tcgatgacag tatcatccag cgccccgcgg
     4321 tcgatggtcg tgcttgcacc aatctcgaca tcatcgccga taatcacgcg gccaatctgc
     4381 ggaatcttga gccagcgtcc ttcatccatc gccatgccaa acccgtcagc gccgatgacc
     4441 acgccggaat gcagaatcac gcgctgtcca atctcacact tgtggtaaac gctgacattg
     4501 gcatgcagcc gtgtcccggc tccgatgcga acctgctcgc cgacgaaaca gtttgcaccg
     4561 atgattgctt cgtcgccgac ttccgcgccg ctttcgatca ccgtacaagg ccccacgctc
     4621 gctgttgcgg ccaccctggc tcgtgcatcg acgactgctt gcgcatggat gccggcgggc
     4681 gggcggcttt ccggattgat cagggcagct atgcgcgcat agctggcata aggattcttg
     4741 cacacgatgc gcggcaaccg cgcagccgcg gcgtagtctt ccgccagcag aactgcgccg
     4801 gcacgcgtcg tggacaggag cttttggtat ttacggttag tgaggaagga aagctcttgg
     4861 ggaccggccg tttccagcgg cgcaacctga tggatacgca cgtcggggtc tccaatcagg
     4921 gtcccgccga agcgcccggc tagttcagcc agactgaatg atctcccctg ttcccctttc
     4981 attggagccg ggtcgctgtg cttaccttct gaaccgcggg cgggcgtcca atgatgatcg
     5041 ccccgctatt tgtcggcgag cgccttgatc actcggtcgg tgatgtcgat gcgcggactg
     5101 atataaaccg cctcttgcag aatgagatca aacttctcgc tttcggcaat ctgctggatg
     5161 accttgttag cgcgttcgag cactgcggcg agttcttcgt tcttgcgcag gttaagatct
     5221 tcgcgtagtt cgcgctgcat gcgctgcaga tcgcggttca tccgggccag gtcctgctcc
     5281 ttgtcgcgcc gctcggactc gcccatcgtc acgccgtcct tttccaggct ggtttgcaaa
     5341 tcgcgcgcct gcttggcaag cttttgcagt tcctggtcgc gcgaagcgaa ttccttctcg
     5401 atcttctttt gcgccttgac cgccggcgcc gcttccttga agagcctttc gagctttacg
     5461 aaaccgacct tgagctctgc ggcaccggcg aagtttgcat aggccagcag cgccgcagcg
     5521 gcaagtacca gcgggaattt attcaacttc tgttctccta tcgtgtggtc ggcacatggc
     5581 taaaaaaccg tccccaaagt gaactggacc ggctgcctcc ggtcctcggg tctgggattg
     5641 attgctctgg caaagcttat tttcatgggg cctaccggag agtaccagtt gaatgaaaga
     5701 ccggttgaat agcgcgtctc cgacaagtca tattgctcac cgagggtacc ggcatcaatg
     5761 aaccagctca ggcgcactga cttgtccttt tgcaagccgg gaaacggaaa cagcacttcc
     5821 acgcttccgt tcagcaaatg gctgccgcca agcgcgtctc cgttggtgtc cctgggacca
     5881 acggacgcag tctcgtaacc acgcaacgaa ttcacgccac ccagataaaa caccttgaaa
     5941 aatggcaatg gcttttgctg atatccggcg gcgtatccga tctggccgct cgtgtaaaac
     6001 accaggtcgc gagtcagcgg atgataccac tgaacctgat aagtggtccg gtagtaggtg
     6061 agttccgcag tgggcaggcc gatctccgcg gaaacccgct gcagggtacc gctggtcgtg
     6121 tagatcacgc tgtcacgctt gtcccgcgcc caaccgacgg tggccgggtt gctgttgata
     6181 tgcgagccga attccttcac gaagtcgaca tagcgttgcg gtgtattagc gaacagaccg
     6241 atttgcgttt gctcgtaggc aagcccgtaa ttgatggtat caaattccgt gatcggcacg
     6301 ccgaagcgca cgccaccacc gatggtcgac gtgttatacg tggcgatacc actcaggttc
     6361 gccgtatcga ccctgcgctt gtaaacatcg aatccccggc tcaccccatc gtccgtgtaa
     6421 tacggattgg tgtacgacac ggagtaaatc tggttgacgc tgccggaact tacctggaat
     6481 gacaatgcat tgccggtgcc gaagatattg ctctgggaaa cgcctgcgga aagaataact
     6541 ttttccgccg tcgaatagcc gatgcccgca gtaagattgc cggtcgcacg ctcggtgact
     6601 ttgacttcga cgtcgacttg atccgtagta ccgggcacag ccggcgtatc cacctggacc
     6661 tccgaaaaat agcccagctt gtcgatccgt tgtttcgacc gattga
//
LOCUS       DATMZQ010000487         4598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1607699, whole genome shotgun sequence.
ACCESSION   DATMZQ010000487 DATMZQ010000000
VERSION     DATMZQ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607699"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VN496_15555"
     CDS             <1..184
                     /locus_tag="VN496_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18424.1"
                     /translation="YQYRNMKLASGLLSATGAFDVSPSKNVSGRITVELRSQAAQIKG
                     NFIVDGDLKAVVLRPN"
     gene            complement(188..760)
                     /locus_tag="VN496_15560"
     CDS             complement(188..760)
                     /locus_tag="VN496_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXF18425.1"
                     /translation="MTITGILLAAGTGSRFGANKLLHPLPDGTPIAIAALRTLKGALP
                     DVIAVIRPVDGRLRALLDDEQVAVHLCNDAHLGMARSFVCGIEASRDADAWVIALADM
                     PFVQPRTIKSIAERVAQTGRIVIPAYLGQRGHPVGFGRRYLDELQKLIGDEGARSVIG
                     RHPDDVELVESDDSGILRDIDTQADLQRTS"
     gene            complement(886..2220)
                     /locus_tag="VN496_15565"
     CDS             complement(886..2220)
                     /locus_tag="VN496_15565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXF18426.1"
                     /translation="MISSIFGQRHVGRFSTLLLCALVTGLPIAWPGAGYCAIQIASAR
                     VWPAQEYTRLTLESDAPVTYSVFTVATPARLVLDLEDVEFNTALEQLPAKISPTDPYV
                     KALRIGRFKPGIVRLVLDLKAQVKPQVFVLKPVGDYGHRLVLDVYPLIEADPVMALLD
                     KKPAASLASPSQDVPQKPDRPSATFTGKALSVAPNPVQKPVASPDVSRLIMVAIDAGH
                     GGEDPGARGRSGTHEKHVTLAIARKLKALVDEEPNMRGMLIRDGDYFIPLVGRVMKAR
                     KIQADLFVSIHADAYVKPHARGSSVFALSERGATSAAAGWLARKENEADLIGGVNIDV
                     ADPTLKQVLIDLSQTATINDSLKLAKAVLGELGGVNDLHKPHVEQAGFAVLKAPDIPS
                     ILVETAFLTNPEEEKKLKDEKYQDKIAQAILVGIRKYFSANPPLARSKLAQN"
     gene            complement(2217..2693)
                     /gene="tsaE"
                     /locus_tag="VN496_15570"
     CDS             complement(2217..2693)
                     /gene="tsaE"
                     /locus_tag="VN496_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXF18427.1"
                     /translation="MIDSTDRMLSIFLPDEAATLRLGRRLASVIEPGLYIALSGNIGT
                     GKTTLSRGILRGLGYPGKVKSPTYTLVELYNLSKIDLYHFDFYRFNDPQEWADAGFRD
                     YFTPHNVCLVEWPERAHGLLPVPDIAIALSEETGARRARIGTETERGRHCLEQLQE"
     gene            complement(2690..3610)
                     /locus_tag="VN496_15575"
     CDS             complement(2690..3610)
                     /locus_tag="VN496_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXF18428.1"
                     /translation="MDLRHLRYFVTVAEELHFGRAAKKLHISQPPLSMQIRALENELG
                     VTLLNRTQRHVSLTQAGAALLGEARHILARVEQAVLATKRAGRGEIGELAIGFISVAD
                     YNVLPLVLREFRRRFPLVNLSLRELTTDAQIADLLAGRIDVGFVLPPIDEPALQSVPI
                     LREPLIAALPGKHPLAREPGKLALEKLRDAPFILFPRPNAPGLYDDVVSCCKTAGFSP
                     RVAQEAIQMQTIISLVSAELGVALIPASLTNLQRTGVTYKTLQGGSPLTEVHLAWRRG
                     DDLPVLRVFVDVAVKTARALGKAPKGRAIR"
     gene            3750..>4598
                     /locus_tag="VN496_15580"
     CDS             3750..>4598
                     /locus_tag="VN496_15580"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXF18429.1"
                     /translation="MADNWRSKLITAGVARSPNRAMLRAVDFNDGDFEKPIVGVANGH
                     SNMNPCNAGIQPLVDRAMSALRKAGAMPQVFGTPTVTDGIGMGTDGMKYSLVSREVIA
                     DAIETSVNGQMMDGVLVVGGCDKNMPGGMMAIARMNVPAIYVYAGSIKPGHWKGQDLT
                     IASAFEAVGAFSAGRISKEDYDGIEKNACPTVGACGGMFTANTMSTSFEALGMSLMQS
                     STMASPDAEKADSAAESAKVLVGAIKKNIRPRDIITRKSIENVVSLVMATGGSTNAVL
                     HYLAIAS"
BASE COUNT         1002 a         1307 c         1335 g          954 t
ORIGIN      
        1 ctatcagtac cgcaacatga agctcgcttc aggactattg tcggcgactg gtgcattcga
       61 cgtttcacca agcaagaacg tcagcggcag aataacggtg gaactacggt cgcaagctgc
      121 gcagataaaa ggaaacttca ttgtggacgg cgacctgaag gcggtcgtcc tcaggccgaa
      181 ctgatcatca actggtcctc tgcaggtcgg cctgcgtatc gatgtcccgt aaaattccgc
      241 tgtcatccga ctcaacaagt tccacgtcat cgggatgacg cccgatgact gaccgcgccc
      301 cttcgtcccc aatgagtttc tgcagttcat ctaagtagcg acggccgaac cccaccgggt
      361 gaccgcgttg cccaaggtat gccgggatga cgatgcggcc ggtctgcgcg acgcgttcgg
      421 ctattgattt tatggttcgc ggctgtacga agggcatatc ggctagcgcg atgacccatg
      481 catccgcatc gcgactggcc tcgattccac aaacgaaact gcgcgccatg cccagatggg
      541 cgtcgttgca caggtgaacg gcaacctgtt catcatcgag caaagcgcgc aaacggccgt
      601 cgactggacg gattacggca atgacgtcgg gcaatgcgcc tttcagtgtg cgtagtgccg
      661 caattgcaat tggggtaccg tccggcagcg ggtgcaacaa cttgtttgcg ccgaagcggc
      721 tgccggtgcc cgcagcgagc aggatgccgg tgatcgtcaa gacgttcgca ctggaaatac
      781 agcaatcgtc ctgacgtagc caaactcaaa ctcgtgatgc agcgctagtc tgcactgatt
      841 gcgacgtcga atcgcaactg gatctttcat ggtacgcgtc gaagatcagt tctgcgccag
      901 cttcgatcgt gccagcgggg gattcgctga aaaatatttt ctgattccaa ccaggatggc
      961 ttgcgcgatc ttgtcctgat atttttcatc cttgagtttt ttttcttcct cgggattggt
     1021 caaaaacgcg gtttcgacca ggatggaggg aatatccggc gccttcagaa ccgcgaaccc
     1081 ggcttgttcg acatgtggct tatgcaaatc gtttacgccg ccgagttcac cgagcaccgc
     1141 tttggcaagt ttgaggctgt cgttgatggt cgctgtttgc gacaggtcaa tcagtacctg
     1201 cttcagggtg ggatcggcca catcgatatt tacgccgcca atcagatccg cctcgttttc
     1261 cttccttgcg agccatccgg ctgccgcaga agtcgcgccc cgctccgata acgcgaacac
     1321 cgatgatccg cgcgcgtgcg gcttgacata agcatcggcg tgtatggaaa caaacagatc
     1381 cgcctgtatt ttgcgtgcct tcatgacgcg cccgacgagc ggaatgaaat aatcgccgtc
     1441 acggatcagc atacctcgca tattgggctc ctcgtccacc agggccttca atttcctcgc
     1501 gatcgccagg gtcacatgct tctcatgcgt accgctgcgt ccgcgcgcac cgggatcttc
     1561 gccgccatgg ccagcatcga tggcgaccat gatcaaacgc gagacatccg ggctggctac
     1621 cggcttctgt accggatttg gcgcgacgga cagcgctttt ccggtgaaag tggccgacgg
     1681 cctgtctggt ttttgcggga cgtcctgcga cggagatgct agcgaagcag cgggtttttt
     1741 atccagcagc gccattaccg ggtccgcctc gatgagcgga taaacgtcta gcacgaggcg
     1801 gtgaccgtaa tcgccaaccg gcttgagcac gaacacctgt ggttttacct gtgccttgag
     1861 atcgagtacc aagcgcacga taccgggttt gaagcggcca atgcgcaacg ccttcacgta
     1921 gggatccgtg ggagatatct ttgcgggaag ctgttcgagc gcggtgttga attccacgtc
     1981 ctcgagatcc agcaccagac gcgccggcgt ggcgaccgtg aagacgctgt atgtgaccgg
     2041 ggcatccgac tccagcgtaa ggcgcgtgta ttcctgtgcc ggccatacgc gggccgatgc
     2101 aatctggatc gcgcaatatc ccgcaccggg ccatgcgatt ggcaaacccg ttaccagcgc
     2161 gcacagcaat agtgtgctga agcgaccaac atggcgttgg ccgaagatcg aggaaatcac
     2221 tcttgcaatt gctctaagca atgtcttccg cgctccgtct ccgtgccgat gcgcgcgcgg
     2281 cgcgcgccgg tttcttcact taatgcaatg gcaatgtccg gcacgggcaa cagaccatgc
     2341 gcgcgttccg gccattcaac aagacacaca ttatgcggtg tgaaatagtc acgaaagccc
     2401 gcgtccgccc attcctgtgg gtcattgaaa cgataaaaat caaagtgata taagtctatc
     2461 ttagacagat tatagagttc aaccagagta tacgtcggac ttttcacctt tccggggtag
     2521 cccagccccc gcagaatgcc ccgactcagt gtggttttgc cggtaccgat gttgcccgaa
     2581 agggctatat acagcccggg ctcgatgacg gaggccaagc gccttccgag gcgcaacgtg
     2641 gccgcctcgt ccggtagaaa aatggaaagc atccgatccg tggaatcgat catcgaattg
     2701 ctcgtccttt gggcgccttc cccagagccc gtgccgtttt gacggcaacg tcgacaaaca
     2761 cacgcaacac cggcaaatca tcgccacggc gccacgcgag atggacttct gtcagagggc
     2821 tgccgccttg caatgtcttg taagtcacac cagtgcgctg cagattggtg agtgatgcgg
     2881 ggatcagggc aacgccgagt tcggctgata ccaggctgat gattgtttgc atctgaatag
     2941 cttcttgtgc aactcgaggg ctgaacccgg ctgtcttgca acaactgacc acgtcgtcgt
     3001 aaaggcccgg agcattcggt cggggaaaaa gaatgaaagg cgcgtccctc agcttttcga
     3061 gtgcaagctt gcccggttcc cgcgcaagcg ggtgtttccc cggcagagcg gcaatgagcg
     3121 gctcgcggag tatcggtact gattggagcg ccggttcgtc aatgggcggg agcacaaaac
     3181 cgacatcgat gcggccagcg agcaggtccg caatttgcgc gtccgtcgtc aactcccgca
     3241 agctcaggtt gaccagcgga aagcgacggc ggaattcgcg caggacgagc ggcagcacat
     3301 tgtagtcagc cacgctgatg aacccgatgg caagttcacc aatttcccct cgccccgccc
     3361 gcttcgtggc cagtaccgcc tgttccacac gcgccaggat atgccgcgct tcaccgagca
     3421 aagctgcacc ggcttgggtc agtgaaacat ggcgctgcgt gcggttgagc agggttacac
     3481 ccagttcatt ctccaatgcg cggatctgca tggatagcgg tggctgtgaa atatgcagtt
     3541 ttttcgctgc acggccgaag tgcaattcct ctgctacggt cacgaaataa cgcaggtggc
     3601 gaaggtccat tgattcgcaa ggaatatcaa agcgacgcca ataatatatt agacgccgcc
     3661 agatcgggca ctcaaactgc gctgggacag agtcgcattt taaaaaaata cgcaagcaga
     3721 attgcgcaac cacttacgtt gggggaaaca tggcagacaa ttggcgcagc aaactgatta
     3781 ccgcgggcgt ggcccgttcg cccaaccgag caatgctccg tgcggttgat ttcaatgatg
     3841 gcgatttcga aaaaccgatc gtcggcgtag ccaatggcca ttccaacatg aatccgtgca
     3901 acgccggtat ccagcctttg gtggatcgcg cgatgagtgc cttgcgcaag gcaggggcga
     3961 tgccgcaggt cttcggcacc ccgaccgtta ccgacggcat tggcatgggg accgacggca
     4021 tgaaatattc gctggtgtcg cgcgaagtca tcgcggacgc aatcgaaacg tccgtcaacg
     4081 gacaaatgat ggacggcgtc ctggtggtcg gcggctgcga caagaacatg ccgggcggca
     4141 tgatggcgat tgcacgtatg aatgttccgg ccatttatgt ctatgccggc agcatcaaac
     4201 ccgggcattg gaagggacag gacctcacca ttgcgagtgc gtttgaagca gtaggggcct
     4261 tcagcgcggg caggatcagc aaggaagact acgacgggat cgagaagaat gcgtgtccaa
     4321 cggtcggagc ctgcgggggg atgtttactg ccaataccat gtccacctcg tttgaagcgc
     4381 tcggcatgag cctgatgcag tcatcgacca tggcttcccc cgatgccgaa aaagccgact
     4441 cggcggcgga gtctgcgaaa gtgcttgtcg gtgccatcaa gaagaacatc cgtccccgcg
     4501 acatcattac ccgaaagtcg atcgaaaacg tggtgagtct ggtgatggct acgggcggtt
     4561 ctaccaacgc ggtgctgcac tatctggcga ttgcgtcg
//
LOCUS       DATMZQ010000488         5152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4779743, whole genome shotgun sequence.
ACCESSION   DATMZQ010000488 DATMZQ010000000
VERSION     DATMZQ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4779743"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VN496_15585"
     CDS             <1..1488
                     /locus_tag="VN496_15585"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXF18430.1"
                     /translation="FVTENGAETDLDLGHYEHFAAAKMTKRNNFTTGQIYESVIKKER
                     RGDYLGGTVQVIPHITDEIKSFIRMGAGDAEVAICEIGGTVGDIESLPFLEAVRQMSI
                     QNGRGNSCFIHLTLVPFIPSAGEIKTKPTQHSVKELREIGIQPDILLCRADRMVPDNE
                     RRKIALFTNVEEEAVISAVDVDSIYKIPAFFHDQGLDEIVCRKLGIKAPPADLSKWQQ
                     LLEALANPLHEVDIALVGKYVDLADSYKSLTEALIHAGIHTRTKLNVHYIDSESIEKN
                     GLECLEKMDAILVPGGFGQRGVEGKIAAIRYAREHKIPYLGICLGMQLATIEFARNVA
                     GLAGANSTEFDADTPHPVVALITEWLDRTGRVERRTGQSDLGGTMRLGAQSCPVEPGT
                     LAAHIYGSRVNERHRHRYEVNNAYVPLLEAKGYKVSARTPSENLPEMMELTGHPWFVG
                     CQFHPEFTSTPRDGHPLFSAFINAAVRYRESDSQTERAAKIKSVA"
     gene            1895..2749
                     /gene="kdsA"
                     /locus_tag="VN496_15590"
     CDS             1895..2749
                     /gene="kdsA"
                     /locus_tag="VN496_15590"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXF18431.1"
                     /translation="MKLCGFDVGLERPLFVIAGPCVVESRQMAFDTAGELKQMTAALG
                     ISFIYKSSYDKANRSSGKSFRGLGVDKGLEILADVRKQIGVPVLTDVHAQEEIAAVAA
                     VVDVLQTPAFLCRQTDFIRAVAMSGKPVNIKKGQFLAPGDMKNVVDKAREASGQDNIM
                     VCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTSSGGQREFVPVL
                     ARAAVATGVAGVFMETHPNPEKALSDGPNAWPLDRMKALLGTLKALDELVKSRGLAES
                     ELMAQRGR"
     gene            2779..4068
                     /gene="eno"
                     /locus_tag="VN496_15595"
     CDS             2779..4068
                     /gene="eno"
                     /locus_tag="VN496_15595"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXF18432.1"
                     /translation="MSSIVDIVAREILDSRGNPTVEADVLLESGVSGRAAVPSGASTG
                     TREAIELRDGDKSRYGGKGVLKAVEHINTEICESIVGLDAVEQCFIDQTLIDLDGTEN
                     KSRLGANAILAVSLAVAKAASEESGLPLYRYLGGAAKMQLPVPMMNVINGGAHANNSL
                     DLQEFMIIPWGAESFREAMRCGAEIFQTLKKLLDQKGLSTTVGDEGGFAPNLPSNEAA
                     LQLIIEAIDAAGYTPGSQVAIGLDCASSEFFKDGKYDIASEKRMLGAVQLTDYLSSWA
                     DKYPIVSIEDGMAEGDWEGWKILTDRLGKKVQIVGDDVFVTNTRIIAEGIRQGIANSV
                     LIKINQIGTLTETLAAIEMAKRAGYTAVVSHRSGETEDTFIADLAVGANALQIKTGSL
                     SRSDRLAKYNQLLRIEEDLGDSASYAGRDAFYQLNRA"
     gene            4073..4381
                     /gene="ftsB"
                     /locus_tag="VN496_15600"
     CDS             4073..4381
                     /gene="ftsB"
                     /locus_tag="VN496_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HXF18433.1"
                     /translation="MRFLTLALLLLIALIQNPLWFGKGGWLRVHELAGQVDAQRDANV
                     KLAARNATLEAEVRDLKQGYEAIEERARYELGMVKQDEVFFQLLQPPRPTSSKLPEKH
                     "
     gene            4428..>5152
                     /locus_tag="VN496_15605"
     CDS             4428..>5152
                     /locus_tag="VN496_15605"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXF18434.1"
                     /translation="MRKWSGVALVTVALVITVLAVNTVRLRSRQVNVAPNAEVPPPQA
                     AAALRLAQAVRFRTISAQQPSSFAGAEFDHFRAFLAARYPRLGRVLRKRLINDYSLLY
                     EWPGTDASLKPVLLMAHQDVVPVDPASADQWLQAPFGGVISGGFIWGRGTLDDKGALM
                     ALHEATETLLAQGFQPARTVYFAFGHDEEVGGRQGSLKIAEWLASQGVRLESVLDEGQ
                     VVTEGIVAGISKPVALIGIAEKG"
BASE COUNT         1149 a         1405 c         1606 g          992 t
ORIGIN      
        1 ttcgtgaccg agaatggcgc tgaaacagat ttggaccttg gccactatga gcacttcgcc
       61 gctgcgaaga tgaccaagcg gaacaatttc accacggggc agatctacga gagtgtcatc
      121 aagaaagagc gccggggcga ttatcttggc ggcaccgtgc aggtcattcc gcacatcact
      181 gacgaaatca agagcttcat ccgcatggga gcgggggacg ccgaagtagc gatttgcgaa
      241 attggcggca cggtcggcga catcgagtcc ctgccgttcc tggaagccgt gcgccaaatg
      301 agcattcaga acggcagagg caattcgtgc ttcatccatc tgacactggt gccattcatt
      361 ccatcggctg gggaaatcaa gaccaaaccg acacagcact cagtcaagga attgcgtgag
      421 atcggtattc agcccgatat cctgctgtgc cgcgcagacc gcatggtgcc agacaacgag
      481 agacgcaaga tcgcgctgtt tacgaacgtc gaggaagaag cggtcatttc cgctgtagac
      541 gtggactcaa tttacaagat ccccgctttt tttcacgatc aggggctaga tgagatcgtt
      601 tgccgcaaac tcggcatcaa ggcgccgccg gccgacctgt cgaaatggca acaattgctc
      661 gaggcacttg ccaatccgct tcatgaagtt gatatcgcgc tggtgggcaa atatgtcgat
      721 cttgccgact cgtacaaatc gctcacggaa gctctcatcc acgccggaat ccatacgcgc
      781 accaagctca atgttcatta cattgattcg gaaagcatcg agaagaatgg tttggagtgc
      841 ctggaaaaaa tggacgcgat tctggtgccg ggcggctttg gccaacgcgg tgtagaaggc
      901 aagatcgcag cgatccgtta tgcacgcgaa cacaagattc cttatcttgg catctgcctc
      961 ggaatgcagc ttgccacgat cgaattcgcg cgcaacgtcg ctgggcttgc gggtgccaat
     1021 agtacggaat ttgatgcgga taccccgcat cccgtggttg cactgattac tgaatggcta
     1081 gaccgtaccg gccgagtgga gcgccgaaca ggccagtcgg accttggcgg caccatgcgc
     1141 cttggcgccc aatcctgccc ggttgaaccg ggcacgctgg cggcccatat ttacggcagc
     1201 cgggtcaacg agcggcatcg ccatcgctac gaagtcaaca atgcgtacgt accccttctc
     1261 gaggcaaagg gctacaaggt ttcagcgcgc acgccgagcg aaaacctccc ggagatgatg
     1321 gaactgaccg ggcatccgtg gttcgtcgga tgccaattcc atcccgaatt cacgtccacc
     1381 cctcgcgatg gtcatccgct gttttcggct ttcatcaacg ccgccgtgcg ctatcgcgaa
     1441 agcgatagtc aaacggagcg agcagccaag atcaagagcg tcgcctgaag atgaagctgt
     1501 gcgggtttga agtagggctc gagcggcctc ttttggtgat tgcggggagt gttgtagcgc
     1561 ggagcagggc cccccacggg ggatgcgccc cagactcggt acgcggagcg cgagtgcggg
     1621 aaggggcgca caaggctgcg gctcaattcc ccaaattggg gcgcatcgcg tctgttcccg
     1681 caaacgggcc tgcggccaac gtgtcgcagc cgcaccgcga agaagctcgt gagggcccgc
     1741 agcgatgagg tcaccatgaa actctgtggg ttcgatgtcg ggctggagcg gccactcttt
     1801 gtgattgcgg ggggcgtcgt agcgcggagc aggggcccat tcaggggatg cgaccgtgca
     1861 gacgctcgtg aggccccgca gcgatgaggt caccatgaaa ctctgtgggt tcgatgtcgg
     1921 gctggagcgg ccactctttg tgatagcggg cccctgtgtg gtcgaatcaa ggcaaatggc
     1981 cttcgacaca gcgggagagc tcaagcagat gactgcggca ctgggcatat cgttcatcta
     2041 caagtcatcg tatgacaagg cgaaccgaag ttccggcaaa tcattccggg gccttggcgt
     2101 ggataagggg ctcgaaattc tggcggacgt cagaaagcag atcggcgtgc cggtgctgac
     2161 cgatgttcac gcgcaagaag aaatagctgc ggtcgccgca gtagtggacg tattgcagac
     2221 gcccgccttt ctgtgccgcc agaccgactt cattcgcgcg gtggcgatga gcggcaagcc
     2281 cgtcaacatt aaaaaaggcc agttccttgc ccccggagat atgaaaaacg tggtcgacaa
     2341 ggcgcgcgaa gccagcggac aggacaacat catggtatgc gagcgcggtg cttcgttcgg
     2401 ctacaacaat ctggtctcgg acatgcgttc actggcgatc atgcgagaga ccggatgccc
     2461 ggtcgtgttc gacgcgacgc attcggtaca gcttcccggt ggtcaaggta cctcgagcgg
     2521 cggccagcgc gagttcgtgc cggtactggc gcgagccgct gtggcaaccg gtgtcgcagg
     2581 ggtattcatg gaaacgcatc cgaaccccga gaaggcgtta tcggacggac ccaacgcatg
     2641 gccactcgac aggatgaagg ctctgcttgg gacattgaag gctctcgatg aactggtgaa
     2701 atcccgcgga cttgctgaaa gcgagctgat ggcgcagcgg ggccgataag caaacagacg
     2761 catcaaagga aaaaaagaat gagttccata gttgacattg ttgcccgtga gattctcgac
     2821 tcacgcggca accctaccgt cgaggcggac gtgctgctcg aatccggcgt gagtggccgc
     2881 gccgccgtgc cgtcgggagc ttccaccggc acgcgcgaag caatcgaatt acgcgacggc
     2941 gacaagagcc gctatggcgg caaaggcgtc ctgaaggcgg tcgagcatat caataccgaa
     3001 atctgcgagt cgatcgtcgg actggacgcg gtggagcaat gtttcatcga tcagacgctg
     3061 atcgatctcg acgggactga aaacaaatcg cgactgggtg caaacgcgat actggcggtt
     3121 tcgcttgcgg tagccaaggc ggcctccgag gaatccggat tgccgctcta tcgttatctg
     3181 ggtggagcgg ccaagatgca gctgccggtg ccgatgatga atgtgatcaa cggcggagcg
     3241 cacgccaaca acagcctgga tctgcaggaa ttcatgatca ttccctgggg cgcggagagt
     3301 ttccgggaag ccatgcgttg cggggcagaa attttccaga cgctgaagaa gctgctagat
     3361 cagaaaggtt tgtccaccac agtaggtgat gaaggcggtt tcgcgcccaa cctgccgagc
     3421 aatgaagccg cgctgcaact gattatcgaa gccatcgatg cagcgggcta tacccccggg
     3481 tcgcaggtgg caattggcct ggactgcgcc agctctgagt tcttcaagga tggcaagtac
     3541 gatatcgcct cggaaaaacg catgctgggc gcggtgcagc tgactgatta cctatcgagt
     3601 tgggcggaca agtatccgat tgtgagcata gaagacggta tggcggaggg tgactgggaa
     3661 ggctggaaaa tactgacgga ccggcttggc aagaaagtgc agattgtcgg cgacgatgtc
     3721 tttgtgacca acaccagaat catcgcggaa ggcatccgcc agggcattgc aaattccgta
     3781 ctgatcaaga tcaaccagat cggcacgttg actgaaacgc ttgccgcgat cgagatggcc
     3841 aagcgcgccg gctacaccgc cgtggtctcg caccgctccg gcgaaacgga agacacgttt
     3901 attgcagacc ttgcggtagg ggcaaatgcg ctgcaaatca agaccggatc gctgtcgcgc
     3961 tcagaccgcc tggcaaaata caatcagttg ctgcggatcg aagaagacct gggcgactcc
     4021 gcaagctatg ctggccgcga tgctttctac cagttgaatc gcgcctgatc gaatgcgctt
     4081 cctcaccctg gcactattgc tgctgatagc cttgatccag aacccgctgt ggttcggcaa
     4141 gggtggctgg ttgcgcgtgc acgagcttgc cggtcaagtg gacgcacaac gggatgccaa
     4201 tgtaaagctc gcggcgcgca acgcgacact cgaagccgaa gtgcgcgatt taaagcaagg
     4261 ttacgaagcc atcgaagagc gggcgcgcta cgagctgggc atggtcaagc aggacgaagt
     4321 gtttttccag ttgctgcagc caccgcggcc gacttcttca aaactgcccg aaaagcactg
     4381 acgtttgttc tgcaagttcc gaattccggc ggcgttgcgg agggccgatg agaaaatggt
     4441 cgggcgtagc cctggtaacg gttgcgcttg tcataacggt actagcggtc aacacggtac
     4501 gcctccgtag caggcaagtt aacgtagcgc caaacgcgga ggtccctccg ccgcaggctg
     4561 cagcggcgct acgtctcgcg caggcagtgc gcttccggac aatttccgcc caacagcctt
     4621 ccagctttgc gggtgcagaa tttgatcatt ttcgcgcctt ccttgctgca cgctacccgc
     4681 gcttgggaag ggtgctgcgc aagcgactca tcaacgatta cagcctgctg tacgaatggc
     4741 ccggcacgga tgcttcattg aaaccggtgt tgctcatggc gcatcaggac gtagtaccgg
     4801 tggacccggc cagcgcggac cagtggcttc aggcgccgtt tggtggagtg atctccgggg
     4861 ggttcatctg gggacgcggc acactggatg acaaaggtgc cctgatggct ttgcatgaag
     4921 ccacggaaac cttgcttgcc caaggattcc agcctgcgcg caccgtgtat tttgcatttg
     4981 gtcacgatga ggaagtcggt ggccggcaag gatccctgaa aattgcggaa tggctggcgt
     5041 ctcaaggcgt gagacttgag tcggtgctcg acgagggaca ggtggttaca gagggcatcg
     5101 ttgccggcat cagcaaaccg gttgcgctga ttggaattgc ggagaaaggc ta
//
LOCUS       DATMZQ010000489         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4431292, whole genome shotgun sequence.
ACCESSION   DATMZQ010000489 DATMZQ010000000
VERSION     DATMZQ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4431292"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1155)
                     /locus_tag="VN496_15610"
     CDS             complement(82..1155)
                     /locus_tag="VN496_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF18435.1"
                     /translation="MLDSHHQAELHFPFAAPLKTDELVEVHPGVLWGRLPLPFRLDHV
                     NVYFIDDGDGWAVIDTGIGDELTKAVWQRLLDGPLRGRRLTRLIVTHFHPDHIGLAGW
                     LAERYDVPLLTSQTAYLSCVNISLSPGALDAKIYRDFYLRHGLDAETTAQVATRGHSY
                     LRMVTGLPPTFRRLVAQDVLNIGGRTFEVLSGDGHAPEQLMLHCPAEKLFFAADQVLA
                     KITPNISVWAVEPEGNPLDLYLRSLRSLQASLPSDTLVLPGHQLPFRQPQVRCAELIR
                     HHKERCAAIAAACRGIPRTTAEIVPFVFRRPLDPHQMSFAFSEIQAHVNYMVERGELV
                     WGIGRDDVHTVESTAAPGAAAGE"
     gene            complement(1323..2024)
                     /locus_tag="VN496_15615"
     CDS             complement(1323..2024)
                     /locus_tag="VN496_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF18436.1"
                     /translation="MDTITYKTALIVGAGVGLSAALTRLFTSKGIRVALAARETDKLG
                     ALCKQTGATAYSCDATDLSDLTKLFSDVERDCGAPDVVVYNASARVRGAFVDLVPADV
                     AKAIAVSAFGGFLVAQQASRRMLPNRHGAILFTGASASVKGYAQSAAFAMGKFALRGL
                     AQSMARELSPHGIHVAHFVIDGGIRSAVRTEPPDKPDSLLDPDAIAANYWSVLSQPRS
                     AWTSEMEMRPWLEKF"
BASE COUNT          493 a          645 c          638 g          444 t
ORIGIN      
        1 cgttgcacca atatcgacag gccaaggagc tgctgtcgag attcggctga tctagtttcg
       61 agtaaccggc cgatcaaagg ctcactcgcc ggcggctgca cccggtgcag ccgtagattc
      121 tacagtgtga acgtcgtcgc gccctatgcc ccagaccagc tcgccgcgct cgaccatgta
      181 attgacatgc gcctggatct cgctgaacgc gaaactcatc tggtgcggat ccagcggtcg
      241 ccgaaacacg aagggcacaa tctcagcagt cgtgcgtggt attccccggc aagctgccgc
      301 gatggcggca cagcgctctt tgtgatggcg aatgagttcg gcgcaccgga cttgcggctg
      361 ccggaaaggc aattgatgac cgggtagaac caaggtgtct gacggcaatg aggcttgcag
      421 gctgcgcaat gaacgcaagt agagatcgag aggattgccc tcgggctcga ctgcccagac
      481 gctgatgttt ggcgtgattt tcgccagaac ctgatctgcc gcgaaaaaca atttctcagc
      541 cgggcaatgg agcatcagct gttcgggcgc atggccatca ccgctcagta cctcgaatgt
      601 gcggccaccg atgttgagca catcctgtgc aacaagccga cggaacgttg gcggcaagcc
      661 ggtgaccatt ctgagatagc tatggccacg cgtcgcaacc tgtgccgtcg tttccgcatc
      721 aaggccgtgg cgcagataaa aatcccgata aatcttggcg tcgagtgcgc ccgggctgag
      781 cgaaatattg acgcatgaaa gatacgccgt ttgacttgtc agcagtggaa cgtcgtaacg
      841 ctcggcgagc cagccggcga gaccgatatg gtcggggtga aaatgcgtca cgatgaggcg
      901 cgtgaggcgc cggccgcgca gagggccgtc aagaagcctt tgccaaacgg ccttggtcag
      961 ttcatcgccg atgccagtgt cgatgacggc ccagccgtcg ccatcgtcaa tgaagtagac
     1021 attgacgtgg tcgagccgga aaggcaaagg caaccgcccc cacaagacgc ccgggtggac
     1081 ctcaaccaat tcatcggttt ttagaggcgc ggcgaaggga aagtgcagtt ccgcctgatg
     1141 gtgtgagtcg agcacgtcct tattctcagt ctgcgttcgt tagcgctttt cgtagtttga
     1201 atttgcaagc ccggtcaagc aaccaaagct cacaatcggc accatagcca gcagggccgg
     1261 cagctcgccc tgaccgctgt tcacatactt ttcttgtcgc ggaacaagga ggaaatcgcg
     1321 tactagaatt tttccaacca gggccgcatt tccatttccg acgtccaggc gctgcgcggc
     1381 tggctcaaca cactccaata gttggctgcg atcgcatctg gatcgagcaa cgagtcgggc
     1441 ttgtccggcg gctcggttcg caccgcgctt cgaatgccgc cgtcgatcac gaagtgcgcg
     1501 acgtggatgc catgcggcga caattcacgc gccatgctct gcgcgaggcc gcgcaatgcg
     1561 aactttccca tcgcaaaggc cgcggactgc gcataaccct tcacactcgc tgatgcaccg
     1621 gtgaaaagaa tggcgccgtg acggttggga agcatgcggc gcgacgcctg ttgtgcaacg
     1681 agaaacccac caaaggcgct aacagcgatg gctttggcaa catcagcggg gaccaggtcc
     1741 acaaatgcgc cacgcacgcg cgcacttgca ttgtaaacaa caacgtcagg agcgccacag
     1801 tcccgctcga catcagaaaa tagcttcgtt aggtcgctca aatcggtcgc atcgcaagaa
     1861 taggccgtgg caccggtttg tttgcaaagc gcgccaagct tgtcagtctc gcgcgccgct
     1921 aacgcaacgc ggatgccttt gcttgtgaaa agtctggtca acgcggcgct taagcccacg
     1981 ccagcaccaa cgatcagcgc tgttttgtag gtaatggtgt ccatacatat tctccttcag
     2041 cacacgcaat ttatcctgtt tatctgaaat tcgcggcgca ggaggcgata tctgccatgc
     2101 attggggcga aagtggcaat cactatctat tgaccccgcc ttgtgccctc acccgctttc
     2161 actgatgctg accgccgtcg aatgacagca atcctatggg agaatgatta tgctgttcat
//
LOCUS       DATMZQ010000490         2952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4781023, whole genome shotgun sequence.
ACCESSION   DATMZQ010000490 DATMZQ010000000
VERSION     DATMZQ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781023"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VN496_15620"
     CDS             complement(<1..1798)
                     /locus_tag="VN496_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF18437.1"
                     /translation="MNPSRLFILRPVATSLMMVAILLSGILAYRLLPLSALPEVDYPT
                     IQVVTLYPGASPDVTTSSITAPLERQFGQMPGLNQMSSTSSGGASVITLQFALDLSLD
                     VAEQEVQAAINAATSLLPPDLPSPPIYNKVNPADTPILTVAITSNSLPLPKVQDLIDT
                     RIAQKISQLPGVGLVSLSGGQRPAVRIQANARALASYGMSLDDLRNAIGNANVNLAKG
                     SFDGPARASTIDANDQLKSAGEYRGLIIAYKNGAPIRLTDVADVIDDAENVRLAAWAN
                     DKAALIMNIQRQPGANVIEVVNRIKKLLPQLKASLPGSVDAVLLTDRTTTIRASVKDV
                     QFELMLAVALVVMVIFLFLRNVSATLIPSVAVPLSLVGTFGVMYLAGFSINNLTLMAL
                     TIATGFVVDDAIVMIENISRFIEEGMEPLQAALVGAKQIGFTIISLTFSLIAVLIPLL
                     FMADVVGRLFREFAITLAVAILISAFISLTLTPMMCARLLRHIPEASQSRFYRSSGRF
                     FDMVIARYARALDWVLDRQAATLIVAVATLALTVLLYIFVPKGFFPPQDTGAIQAISE
                     APQSISFAAMAQRQQHLASVVLQDPAVESLSSF"
     gene            complement(1811..>2952)
                     /locus_tag="VN496_15625"
     CDS             complement(1811..>2952)
                     /locus_tag="VN496_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HXF18438.1"
                     /translation="GQSSPGKAGADAMNRSQPVVAAAARVGDINLYLNGLGTVVPLNT
                     VTVRTHVDGELQSVMFREGQTVKQGAVLAQIDPRPYQVQLEQAEGTMARDRALLKNAE
                     ADLERYRTLFEQDSIARQQLDTQASLVRQYQGALRADQAQIDSARLNLTYSRITAPIS
                     GRLGLRQVDVGNIVHANDTNGIVVITQLQPITVVFTLPEDNIAAVMKKMHAGQDLVVD
                     AYDRAGKNRLASGQLLTVDNQIDPATGTIKLKAQFANDDSSLFPNQFVNARMLLDVLH
                     DNILVPAAAIQRGTQGTYVYVIKSDRTVTLRNVKLGPTEGETSAIASGLSSGELVVVD
                     GTDKLREGAKVEIGNSVAGTPPETGLPMHGKRRHDGENSPPASSS"
BASE COUNT          592 a          864 c          922 g          574 t
ORIGIN      
        1 tgaacgacga caggctttcc actgcgggat cctgcagcac gacgctcgca agatgctgct
       61 gacgttgcgc catggcggcg aaagaaatgg actgcggcgc ctcagaaatt gcctgaatcg
      121 cgccggtatc ctggggcggg aaaaatccct tcggcacgaa aatatagagc agcacggtga
      181 gcgccaaggt ggcgaccgcg acgatcagcg tggccgcctg gcggtccagg acccagtcga
      241 gcgctctggc gtagcgcgca ataaccatgt cgaagaagcg gccgctggag cggtagaaac
      301 ggctttggct ggcttccgga atgtggcgca acagtcgcgc gcacatcatg ggtgtcaacg
      361 tcagcgaaat gaaagctgaa ataagaattg cgaccgccag cgtgatggca aattcgcgga
      421 atagccggcc gacgacatcg gccatgaaca gcagcggaat cagaaccgcg atcagcgaaa
      481 aggtcagcga tatgatggtg aagccgatct gcttcgcgcc aaccagtgcg gcctgcaacg
      541 gttccatccc ttcttcgatg aagcgcgaaa tattctcgat catgacaatc gcatcatcca
      601 cgacgaagcc ggtagcgatg gtgagcgcca tcaacgtaag gttgttgata ctgaaaccgg
      661 ccaagtacat gaccccgaac gtgcccacga gcgacagcgg caccgcgacg ctgggaatca
      721 aagtggcgga aacgttgcgc aggaacagga agatcaccat gacgaccagc gctaccgcca
      781 gcatcagttc gaattgcaca tccttgacgg aggcgcggat ggtagtcgtg cggtcggtca
      841 gcaggacagc gtccaccgag ccgggcagcg aggccttgag ctgtggcaac agcttcttga
      901 tccggttcac gacttcaatg acgttggcgc cgggttggcg ctggatgttc atgatcagcg
      961 ccgccttgtc gtttgcccag gccgcaagcc gcacattttc cgcatcgtcg atgacatccg
     1021 cgacgtcagt cagccggatc ggcgctccgt ttttgtacgc gataatcaga ccccggtatt
     1081 cgccggcgga cttgagctga tcgttggcgt cgatcgtcga tgcgcgcgcc ggcccatcga
     1141 agctgccttt ggccaagttg acgttggcat tgccgatggc gttgcgcaaa tcgtccaggc
     1201 tcattccata ggatgccaag gcccgcgcat tggcctgaat gcgcactgca ggtcgctgtc
     1261 cgccggacag gctgaccaag cccacgccgg gcaactgcga tatcttctgc gcaatgcgcg
     1321 tatcaatcag atcctgaact ttgggcagcg gcaacgaatt cgacgtaatt gccaccgtca
     1381 ggatgggcgt gtcggccgga ttgaccttgt tatagatcgg cggcgaaggc aggtctggtg
     1441 gcagcaaaga agtcgcggca ttgatcgcgg cctgcacctc ctgttcggcg acatcgagcg
     1501 aaaggtccaa tgcaaactgc aaggtgatta ccgacgcgcc accggaactc gtggaggaca
     1561 tctggttaag gccaggcatc tggccgaatt ggcgctccag cggcgcggtg atcgaggacg
     1621 tggtgacgtc aggactggcg ccggggtaga gcgttaccac ctggatggtc gggtagtcga
     1681 cttcgggcaa cgccgacagc ggcagcaggc gataggccag aatgccggaa agcaggatgg
     1741 ccaccatcat gagcgaggtg gcgactggac gcaggatgaa cagacgcgac gggttcatgc
     1801 gtccgctgct tcagctgctg gatgcaggcg gcgaattttc accgtcgtgc cggcgcttgc
     1861 cgtgcatcgg caatccagtt tccggcggcg tgccggcaac ggaattgccg atctcgacct
     1921 tggcaccttc gcgcagcttg tccgtgccgt ccacgacgac cagctctcca ctggacaatc
     1981 cggaagcaat ggcgctggtt tccccttccg tcggccccaa cttgacgttg cgcagggtca
     2041 cggtcctgtc gctcttgatc acgtacacgt aagtgccctg cgtcccgcgc tggatggcag
     2101 cggcggggac gaggatattg tcatgcaaga catccagcaa catgcgggcg ttcacgaact
     2161 gattcgggaa cagacttgaa tcatcgttgg cgaattgcgc ctttaacttg atggtgccgg
     2221 ttgcaggatc gatctggttg tccacggtca gcaactgtcc gcttgccaac cggtttttgc
     2281 cggcgcgatc gtaggcgtcg accacaagat cctggccggc atgcattttt ttcatgaccg
     2341 ccgcgatatt gtcttccggc agcgtgaata ccacggtgat cggttgcagc tgcgtgatga
     2401 ccacgatgcc attcgtatcg tttgcatgga cgatgttacc gacgtcgacc tggcgcaggc
     2461 ccagccgccc gctgatgggc gccgtgatac gtgagtaggt caggttgagc cttgcactgt
     2521 cgatctgcgc ctggtcggcg cgcagcgcgc cctggtactg gcgaaccagg gaggcctgcg
     2581 tatccagttg ttgcctggcg atcgagtcct gttcgaacag cgtgcggtac cgttccaggt
     2641 ctgcctcggc atttttcagc agagcacggt cgcgggccat ggtgccttcc gcctgttcca
     2701 gctggacctg atagggacgt ggatcgatct gcgcgagcac ggctccctgc ttgaccgtct
     2761 ggccctcgcg gaacatgacg gactgcagtt caccgtcaac atgcgtcctc accgtaaccg
     2821 tgttcagcgg tacgacggtg cccaggccat tcaggtaaag attgatgtcg ccaaccctgg
     2881 ccgctgcagc aaccaccggc tgactgcggt tcatcgcatc cgcaccggct ttgccggggg
     2941 acgactgtcc cg
//
LOCUS       DATMZQ010000491         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4431541, whole genome shotgun sequence.
ACCESSION   DATMZQ010000491 DATMZQ010000000
VERSION     DATMZQ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4431541"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VN496_15630"
     CDS             complement(<1..629)
                     /locus_tag="VN496_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HXF18439.1"
                     /translation="MLLALCGTVALSVPVFADEAKAADTAPAAAATPAAAAATPAPVP
                     NKGDVSWMLTSTALVLLMSVPALALFYGGMVRSKNMLSMLMQVFVVFSLITVLWCIYG
                     YSLTFTEGNAYFGGLDRLFLNGTFDAIKGEFSMAATFSKATPLPELVYVAFQATFAAI
                     TCCLILGAFAERAKFSAVLIFMVLWFTFSYIPVAHMVWFWPGPDAYTAA"
     gene            complement(658..996)
                     /gene="glnK"
                     /locus_tag="VN496_15635"
     CDS             complement(658..996)
                     /gene="glnK"
                     /locus_tag="VN496_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXF18440.1"
                     /translation="MKLVTAVIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAIKTELLEQVIEAIEKSANTGKIGDGKIFVFDLEQVVRI
                     RTGETGAEAL"
     gene            complement(1058..1846)
                     /locus_tag="VN496_15640"
     CDS             complement(1058..1846)
                     /locus_tag="VN496_15640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HXF18441.1"
                     /translation="MLRNKIIAVAVASAVVLPGMALAADAPAAPASPNTFTANVGFVS
                     DYAFRGISQTMGEPAVQGGFDYTHTSGFYLGIWGSNVSGIQFRDGTLESDWYGGWAKT
                     WGDWGVNVGGLYYYYPGAKLANGDKYDTFEGYGSVSWKWIAAKLSYAFTDFFGANQDS
                     TPGPFNDSSKGSTYIELNATYPINDAFSVLAHIGHQTVEGSTVDLDYTDYKIGVNYLY
                     NGFNFGLAYKDTDADSSLYTMSRANGETVDLGKGKLIASVSKTF"
     gene            2087..2356
                     /locus_tag="VN496_15645"
     CDS             2087..2356
                     /locus_tag="VN496_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HXF18442.1"
                     /translation="METRFLDELNKRISELIAASPARDIEKNMRALLSSAFTRLDLVT
                     REEFDVQQEVLRRSQQKLHDMEARVAVLEAALATYKASGGGEGEA"
     gene            2479..>3131
                     /locus_tag="VN496_15650"
     CDS             2479..>3131
                     /locus_tag="VN496_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HXF18443.1"
                     /translation="MSLAVLYSRALAGMDAPPVTVEVHLANGLPSFTIVGLPEAEVKE
                     SKDRVRSALLNCRFDFPARRITVNLAPADLPKESGRFDLPIALGILAASGQIPGDKLS
                     EFEFAGELALTGELRPIRGALAMTYNAHRSGRAFVLPVESAIEAALVKDARVLPASTL
                     LRVCAHLAGRELLNASAQTPSVVQSEVADLAEVKGQLHAKRALEVAAAGGHSMLMIGP
                     "
BASE COUNT          691 a          877 c          898 g          665 t
ORIGIN      
        1 ttggccgcgg tatacgcgtc cgggccgggc cagaaccaaa ccatgtgcgc gaccggaatg
       61 taagagaagg tgaaccaaag caccatgaaa atcagcacgg cagagaactt cgcccgttcc
      121 gcgaatgcac ccagaataag acagcaggta atcgcagcaa aagtcgcctg gaacgctacg
      181 tataccaact ctggaagcgg ggttgccttg ctgaaggtgg cggccatgga aaactcgccc
      241 ttgatcgcgt cgaacgtgcc gttcaagaac agccggtcaa gacctccgaa ataggcatta
      301 ccctcagtaa acgtcagact gtagccatat atacaccaga gtaccgtgat caacgaaaac
      361 accacgaata cctgcatcag catggagagc atgtttttcg agcgcaccat gccaccataa
      421 aagagtgcaa gtgccggaac gctcatcagc agcaccagcg cggtggaggt cagcatccag
      481 gaaacatcgc ccttgttcgg taccggggct ggggttgcag ccgcagccgc gggtgtcgcg
      541 gccgccgctg gcgccgtatc agcggccttg gcttcgtcag cgaaaaccgg tacggacaat
      601 gcgaccgttc cgcacagggc cagaagcatc agtatttttc taatcatgag attctcccta
      661 tagcgcttcc gcgccggtct cgccggtgcg aatgcgcaca acttgttcaa ggtcgaacac
      721 aaaaatcttg ccgtcgccga ttttcccggt gttggccgat ttttcgatcg cctcgatgac
      781 ttgctccagc aactctgttt tgatcgcggc ttcaattttg actttgggca ggaagtcaac
      841 gacgtactcg gcgccgcgat acagttcggt atgacctttc tgccggccaa aacccttgac
      901 ctcggtcacc gtgatcccct gcacgccgat ggcggacaag gcttcacgca cctcgtcaag
      961 cttgaacggc ttgatgactg cggtaactag tttcatggtt tctccaattg gctttgcgag
     1021 gcggttccgg cgcagtgccg gaaccgttct tttatgtcta gaaagtcttg ctgaccgagg
     1081 caatcagctt gcctttgccc aaatcaacgg tctctccatt ggcccttgac atcgtataca
     1141 gagaagaatc tgcatcggtg tccttgtacg ccaggccgaa attgaatccg ttgtacagat
     1201 agttcacacc aatcttgtag tcggtgtaat caaggtcaac ggtgctgcct tcgacggttt
     1261 gatgacctat atgtgccagg acggaaaacg cgtcgttgat cgggtaggtc gcgttgagtt
     1321 caatatacgt tgaccccttg ctgctgtcgt tgaatggccc gggcgtggaa tcctgattag
     1381 caccgaaaaa gtccgtgaaa gcgtacgata gttttgccgc gatccatttc caggaaacgc
     1441 tgccgtagcc ctcgaacgtg tcgtatttgt cgccgttagc aagcttggcg ccgggatagt
     1501 agtaatacaa accgcccacg ttcacgcccc agtcgcccca ggtcttggcc cagccgccat
     1561 accagtccga ttccagcgtg ccgtccctga attggatgcc gctgacattg gagccccaaa
     1621 tgccgaggta gaagccactg gtgtgggtgt aatcaaagcc gccctgtacc gcaggctcgc
     1681 ccatcgtctg gctgatcccg cgaaatgcgt aatcggatac gaatccgaca ttggcggtga
     1741 acgtattggg cgaggcggga gcggcgggcg cgtccgccgc cagcgccatt cctggtagca
     1801 caacggcact ggccacggca acggcaataa ttttgtttct caacatgggc atcatcctca
     1861 agtggtctat cgaaacatct gacatgcgga aaagacagcg gcatcaggtc agcatttacc
     1921 gtgccaaacg gaaatgagac tcgtatcaaa gaattagcgt gcgccttgag ataattagac
     1981 ggggtggcgc accaaacaaa ggcgccctgc aggcacgctg cactccgtcg gtgcggcaaa
     2041 gatttttaca gcggtttgat acacttcagc cataaggagg tggcctttgg aaaccagatt
     2101 tctcgatgaa ctgaacaaaa ggatcagcga actgattgcg gcctcgcccg ccagggacat
     2161 cgaaaaaaat atgcgggcgc ttctatcgag cgcgttcacc cgccttgatc tggtcacgcg
     2221 cgaggaattc gacgtgcagc aggaagtgct gcgccgttcc cagcagaaat tgcacgacat
     2281 ggaagcgagg gtggcggtgc tggaagccgc tcttgcaaca tataaagcga gcggtggagg
     2341 agaaggcgaa gcatgaaagc cggggcatga gaaccctggg cgccgggatt tcgatctccc
     2401 atcgaactgc ttgcgcatca gcatcggttt tcggtgtctg caacgtcagc tttgcctcgc
     2461 cggctgttgg ccgctgacgt gtcgctcgcc gttctctaca gtcgcgcatt agccggcatg
     2521 gacgcgccgc cggtgacggt ggaagtccac ctggccaatg gcttgcccag ctttacgatt
     2581 gttggcttgc ccgaggccga agtgaaggag agcaaggatc gtgtgcgctc tgcgctgctg
     2641 aactgccgtt tcgattttcc ggcccgccgc attaccgtca atctggcccc tgccgatcta
     2701 ccgaaagaaa gcggccgttt cgatttgccg atagcacttg gcattctggc ggcatcggga
     2761 caaatccctg gcgacaaact gtccgagttt gaatttgccg gtgagcttgc gcttacggga
     2821 gaactgcggc ccatccgcgg cgcgttggcc atgacttaca acgcgcatcg gtcggggcgt
     2881 gcttttgtgt tgcctgtcga aagcgcaatc gaagcagcgt tggtcaagga tgcgcgcgtg
     2941 ctgccggcca gcacgctgct gagagtctgt gcgcatcttg ccggccgtga gttgttaaac
     3001 gcgtctgcgc aaaccccgag cgttgtgcaa agtgaagtgg ctgaccttgc cgaggtcaag
     3061 ggccagcttc acgccaagcg tgcactcgaa gttgctgcag cgggcgggca tagcatgctg
     3121 atgattggac c
//
LOCUS       DATMZQ010000492         4684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4781163, whole genome shotgun sequence.
ACCESSION   DATMZQ010000492 DATMZQ010000000
VERSION     DATMZQ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781163"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VN496_15655"
     CDS             complement(<1..564)
                     /locus_tag="VN496_15655"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF18444.1"
                     /translation="MDAKRVSIAVVFVIALWTAAAALAQGLPAATPESIGMSGQRLAR
                     IGEAFKKEVDQGKLPGAVMLVARKGKLVYAEAVGFQDKEAAKPIAKDSIFRIYSMTKP
                     LVSVAAMMLVEEGRIQLTDPVSKFLPAMKSLNVSVAKADAEYAKITYVQAPADREMTV
                     QDLLRHTAGLAYGEITQNAPVKEALAKS"
     gene            750..1895
                     /locus_tag="VN496_15660"
     CDS             750..1895
                     /locus_tag="VN496_15660"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18445.1"
                     /translation="MKTAISMAAAFALALASHGVPAADPAKSEPIKIGVVNEITGVQA
                     QAGEFTVNGIKLAQEEINNAGGVLGRRIELQIEDNQSTNPGTVLAFSKLGSRGDIAGI
                     VGPIRSTQVQAASPTIAKTGIPTMIGGTDTSLTHVNNRWLFRARPNDSYSSRVIADFG
                     VNTLKLKKWAIIHSTDAFGSGGAKALTAALAAHGVTPVLDQGYTNNSQDFAAVVLSIK
                     KSDADVLSTYMTLEPDVGIFARQLRQLGVTIPWVGSPSLIAVTSLKLAGETLYGTYAI
                     TDFTTDANDLARAFVKRYHDKYGMNPDTFASWSYDATQILARAIRAAGSTDPEAVRSA
                     ILAIKGYKGLEGTYEFDQNGDGLHGYNIVKNEGGKIVFMKRVDFPAQ"
     gene            complement(1967..2374)
                     /locus_tag="VN496_15665"
     CDS             complement(1967..2374)
                     /locus_tag="VN496_15665"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HXF18446.1"
                     /translation="MKNAALLIIGSLLWILPPLDSMAAWLRGGFTGNEMLLHCNAEEK
                     DPVKDFGRGICIGFIDGFVAGHYAAESYHAFHHRDEKIDEIYGHLCIPDTVTRGQLVR
                     TFVQYLEKNAEKLKLPAGLALEDALREAFPCPK"
     gene            2578..3459
                     /locus_tag="VN496_15670"
     CDS             2578..3459
                     /locus_tag="VN496_15670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXF18447.1"
                     /translation="MSHLNTHLLPELLSLSPRAEWTRRDFVGCTLAAGFALAVQPVSA
                     QTIVTDNTGLTAGEVRIPVRGGEIPGYRAMPAGGKDLPLVLVVQEIFGVHEHIRDVCR
                     RLAKLGYIAVAPELFVRQGDVSKLSDMKEIFAIVEKVPDMQVMHDLDDTMSWARKNGA
                     NVTQVAITGFCWGGRIVWLYCEHNPMVAAGVAWYGRLEGQSNDLRPTFPIDIAPKLKV
                     PVLGLYGGADTGISKESIEKMRLALKTAKSKSEIIVYPDAPHAFFADYRPSYRKEAAE
                     DGWKKMRDWLENNLSRV"
     gene            complement(3445..>4684)
                     /locus_tag="VN496_15675"
     CDS             complement(3445..>4684)
                     /locus_tag="VN496_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXF18448.1"
                     /translation="RLGFIGAGFIARFQSVAIHQVRGLEIAGILNRRGAPALAQFCRE
                     QQLGDAHLYGTVAELAKNVDAIAIYVPNYCRVEVMEEIVAAVKSGAKLKGVIVDKPLA
                     RNMREARRMVEMARSANLLTAYFENQIFMKAIQTQRAQLLPVARTMGPLTLTRSAEEH
                     GGPHEPWFWDPTRQGGGVLLDMGCHSIAVGRHVLTPANKGLSFLEPVSVTADLGLLKW
                     GQTKWREKLLKERGVDYAKTPAEDFATGMITYRNPETGQLVKSQFTDSWMFEKQGLRL
                     FMDGTGPGYAFEINTLVSPLNVFIGDVAAEAIADQESALEKATSSRGLLAVQYNEADL
                     YGYTDENRDMLRCFSAGHDAMLSWDYGLEIQRLVMASYLSAERGKKIDLRDKATLAEL
                     DSYVPAIQQGRGADQLLHAG"
BASE COUNT          994 a         1345 c         1351 g          994 t
ORIGIN      
        1 ggacttggca agcgcctctt tcacgggcgc gttctgcgtg atttcaccgt aggccagacc
       61 ggccgtatgg cgcagcaggt cctgcaccgt catttcccga tcggcgggcg cctggacata
      121 ggtgattttc gcgtattcgg catctgcctt tgccacgctg acgttcaggg acttcatggc
      181 aggcagaaat ttcgaaaccg gatcggtcag ctggatgcgg ccttcctcca ccagcatcat
      241 tgccgcgacg gacaccagcg gcttggtcat cgaatagatc cgaaaaatgg aatcttttgc
      301 aatcggcttg gcggcttcct tgtcctggaa accaacagct tccgcgtaca caagcttgcc
      361 ttttcgcgct accagcatga ctgcaccggg caattttccc tgatcgactt ccttcttgaa
      421 cgcttctccg atacgggcca gtcgctggcc cgacatgccg atggattctg gagtagcggc
      481 aggcagtcct tgtgcaagcg ccgcggctgc tgtccacaac gcgatgacga aaaccacggc
      541 tatggaaacg cgctttgcat ccatcacaaa atcctccgcc acatacacaa ggactcgaac
      601 ggtaaggcgc gcgcctgatg gtgtcaatcc ggtcgcaaca acaagttcga agccgaatcg
      661 ctgcacggtc ggccgcgtga tggtgcagtc gacaccgtgc taggatgaat gaaaatgaag
      721 cgacagttgt tgaagccaac ggaggagcga tgaaaacagc aatcagcatg gccgccgcat
      781 ttgcgctggc cttggccagt catggcgtgc cggccgcgga tcctgccaag tcggagccca
      841 tcaaaatcgg tgttgtgaat gagatcaccg gcgtccaggc gcaggccgga gaattcaccg
      901 tcaacggcat caagctcgcg caggaagaaa taaacaatgc cggcggcgtg ctgggacggc
      961 gcatcgaact gcagattgag gacaaccaga gcacgaaccc gggaaccgtg cttgcatttt
     1021 ccaagctcgg aagtcgtggc gatattgccg gcattgtcgg tccaatccga agcacgcaag
     1081 tgcaggccgc ttcgccaacg atcgccaaga cgggcattcc gaccatgatt ggcgggacgg
     1141 ataccagcct tacccacgtc aataaccgct ggctgttccg tgcgcgtccg aacgacagct
     1201 actcttcacg cgtcattgcc gatttcggcg tcaacacgct caagctgaaa aaatgggcca
     1261 tcatccactc cacggacgcg tttggctctg gtggcgccaa ggcgctgacc gcggcgcttg
     1321 cggcgcacgg cgtgaccccg gtactggacc agggttacac caacaattcg caggatttcg
     1381 cggcagtggt gttgtccatc aagaagtccg atgctgatgt tctttcgacc tacatgacat
     1441 tggagcctga tgttggcatc tttgcgcgcc aattgcgcca gttgggcgtg acgattccgt
     1501 gggtcggttc tccttccctt atcgcggtta cttcgctgaa actggccggc gaaacgctgt
     1561 acggcactta tgcaatcacc gacttcacca cagacgcgaa tgatctcgcg cgcgcgttcg
     1621 tgaaaaggta ccacgacaag tacggcatga atccggatac gtttgccagc tggtcatacg
     1681 atgccacgca aatccttgca agggcgatcc gcgctgctgg cagtaccgac ccggaggccg
     1741 tgcgttcggc cattctggcg atcaaaggct acaaggggtt ggaaggaact tacgagttcg
     1801 accagaatgg cgacgggttg cacggttaca acatcgtcaa gaacgagggc ggcaagattg
     1861 tgttcatgaa gcgggtagat tttccggccc agtaaccttg gcgcccgaaa cagaattgca
     1921 tatggagtcc gcctggcgga tgcaatagat cgctggtgtg accggtttat ttcgggcagg
     1981 gaaatgcttc ccgcaatgca tcctcaagtg caagtccggc cggcaatttg agcttttctg
     2041 cgtttttctc caggtattgc acgaatgtcc ggaccagttg tccacgcgta accgtatccg
     2101 gaatgcataa gtgcccatat atttcgtcta ttttttcatc acggtggtgg aaggcatggt
     2161 aactctcggc ggcgtagtgc cccgcaacga atccgtcgat gaagccaatg cagataccac
     2221 gcccgaaatc ctttaccggg tctttttcct cggcgttgca atggagcagc atttcattcc
     2281 cggtgaaacc gccgcgcaac cacgcggcca tgctgtccag tggaggcagg atccataaca
     2341 aggagccaat aatcaggagc gccgcatttt tcatcgaaag acctgataac cggttatggt
     2401 tggattgcca ctttatccca aacgctgttt tgccacactg gcccgggagt gcaatcgggc
     2461 tggacgagcc aaatggtatc cgcgggaaaa atgcgggccg actggcaatt gaaccatcct
     2521 acggaatttc cggttcaggt gaactgtcag accgttttat tccgggggac ctgcgacatg
     2581 agccatctaa atacgcattt gctgccggag ttgctaagcc tgtcgccgag agccgaatgg
     2641 accaggcggg atttcgtcgg ctgcacgctg gcagcaggct tcgccttggc cgtgcagccg
     2701 gtgtccgccc agaccatcgt caccgacaat actggtctta ccgcgggtga ggtgcgcatt
     2761 ccagtacggg gcggcgaaat tccgggctat cgcgccatgc cggcgggggg caaggatctg
     2821 ccgctcgtcc tggtggtgca ggaaatattc ggcgtgcatg aacatatccg tgatgtctgc
     2881 aggcgccttg caaagctggg ctatattgcc gtcgctccag agcttttcgt gcggcaaggc
     2941 gacgtttcga agttgagcga catgaaggag atttttgcga tcgtcgaaaa agtccccgac
     3001 atgcaagtca tgcatgacct cgacgatacg atgtcctggg cgcgcaagaa cggcgcgaat
     3061 gtcactcagg tcgccattac cggattctgc tggggcgggc gcattgtctg gctgtattgc
     3121 gagcataacc cgatggtggc agccggagtg gcgtggtacg gaaggctcga aggccagtca
     3181 aacgacttgc gaccgacatt cccgatcgat atcgcaccga aactcaaggt gcccgtactg
     3241 ggtttgtacg gcggtgccga caccggcatc agcaaggaaa gcatcgagaa aatgcgattg
     3301 gcgctcaaaa cggctaaaag caaatccgag atcatcgtgt atccggacgc tccccatgct
     3361 ttttttgccg attaccggcc gagctatcgc aaggaagcag cggaagatgg ctggaaaaaa
     3421 atgcgggact ggctggaaaa caatctatcc cgcgtgtagc aattgatcgg ctccccggcc
     3481 ttgctggatc gccggcacat acgaatcgag ttcggcaagc gtagccttgt cgcgcaagtc
     3541 gattttcttg ccgcgttcgg ccgacagata gcttgccatc acgagccgct ggatttccag
     3601 gccataatcc caggacaaca ttgcgtcgtg gccggcggag aagcagcgca gcatgtcgcg
     3661 attttcatcg gtgtaaccgt acagatcggc ttcgttgtat tgcactgcga gcaatccgcg
     3721 gctcgacgtg gctttctcca gcgcgctttc ctggtcggca atggcttctg ctgctacgtc
     3781 gccaatgaac acattcagcg gtgataccag cgtattgatc tcgaaagcgt agcccggccc
     3841 ggtgccgtcc atgaacagcc gcaatccctg cttttcgaac atccacgagt cggtaaattg
     3901 cgatttcacc agctgtcccg tttccggatt gcgataggtg atcatgccgg tagcgaaatc
     3961 ctcggctggt gttttcgcgt agtcgacgcc gcgttccttc agcaacttct cgcgccactt
     4021 ggtctgcccc cacttgagca agccaaggtc cgccgtgacg gacaccggct caagaaatga
     4081 caatcccttg ttcgccggcg tgagcacgtg gcggccaacc gcgatgctat gacatcccat
     4141 gtccagcagc acgccgccgc cctggcgggt gggatcccag aaccagggtt cgtgcggacc
     4201 gccgtgttct tctgccgagc gagtcagggt caacggaccc atggtccgcg ccaccggaag
     4261 cagttgcgcg cgctgggtct gaattgcctt catgaagatc tggttttcga aataagccgt
     4321 caacagattt gccgagcgcg ccatttccac catgcgccgc gcttcgcgca tgttgcgcgc
     4381 gagcggcttg tcgacgatga cgcctttcaa tttcgccccc gactttacgg ccgcaacaat
     4441 ttcctccatg acttcgacgc ggcagtagtt cggcacgtag attgcgattg cgtcgacgtt
     4501 tttggcaagc tctgcgacgg tgccgtacaa gtgcgcatcg ccgagttgct gttcgcggca
     4561 gaattgtgcg agcgccgggg cgccacgccg gttcaaaatt ccggcgattt ccaggccacg
     4621 cacctggtga atggcgaccg attgaaaacg ggcgatgaag cctgcaccga tgaatccgag
     4681 tctc
//
LOCUS       DATMZQ010000493         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4432461, whole genome shotgun sequence.
ACCESSION   DATMZQ010000493 DATMZQ010000000
VERSION     DATMZQ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4432461"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2378)
                     /locus_tag="VN496_15680"
     CDS             complement(<1..2378)
                     /locus_tag="VN496_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXF18449.1"
                     /translation="MARPVNSAVRKVAVLGAGVMGSQIAAHLVNAGVEAILFELPAKE
                     GDPNGNVRKALDSLKKLEPSPLSVPVRADYIQAANYDQDLELLKDCDLVIEAISERID
                     WKKTLYEKVAPYLGESAIFASNTSGLSINSLSEAMPGHVRSRFCGVHFFNPPRYMHLV
                     ELIAGRATDPAILDRLEEFLVTTLGKGVIRAKDTPNFIANRVGVFSILAAFHHTQRLG
                     LGFDEVDALTGSLIGRPKSATYRTADVVGLDTLAHVVNTMKEQLPNDPWHKYYQIPDW
                     FTQLLDQKALGQKTGRGIYRKQGREIQVLDLKQQAYRLSGGEADESVVSILKNKNVAE
                     RFNQLRASQHPQAQFIWSAFRDMLHYCAVHLEAIAENARDLDLAVRWGFGWNQGPLEL
                     WQAAGWQQMANWIAEDIAAGKSMAAVRLPDWATDPQRDGVHRAQGSWSPAQRAYKPRS
                     GLPVYRRQPFPDRLISESSVYGTTVFETDATRLWTTGDDIGILSFKTKMHAIGEDVLD
                     GVMQAIEESEHNFKGLIIWQTEPPFSVGANLSGGGARQSAQKPTAFASMMKKFKRDAQ
                     SAILKAAFKLNVADSLMAGRLEKVEQVVEQFQATSQALRYCMIPTVAAVDGMALGGGC
                     EFTMHCDRAVASLESYIGLVEAGVGLLPAGGGCKELAMRAATEAKGGDVFPFLRNYFQ
                     NVATAQVGKSAEHSRELGFLRSCDRIVMNRFEVLHVAKAELDAMAEAGYRPPLRARNI
                     PAAGRSVIATFKMAMTNMLAGHYISEHDFLIGSKIAYVLCGGDVEAGTLLTE"
BASE COUNT          508 a          708 c          648 g          535 t
ORIGIN      
        1 tcttccgtga gcaaggtgcc agcctcgaca tcgccaccgc acagcacata ggcgatcttg
       61 ctgcctatca gaaaatcatg ctcggatatg taatggccgg ccagcatgtt ggtcatggcc
      121 atcttgaacg tcgcaatgac gctgcgtccg gcggctggaa tgtttcgggc tcgcaaagga
      181 gggcggtagc cggcctcggc catcgcgtcg agttccgcct tggcgacatg caagacttca
      241 aaccggttca tgacgatacg gtcgcaggac ctcagaaaac caagctcgcg agaatgctct
      301 gcactcttgc cgacttgcgc cgttgctacg ttctggaagt aatttctcag aaaagggaat
      361 acatcgccac ctttggcttc ggtcgccgcg cgcatggcca gttctttgca accgccgcca
      421 gcaggcaaca aaccgacccc ggcttccacc agtccgatgt agctttcgag cgatgctact
      481 gcccgatcac agtgcatggt gaactcgcag ccgccgccca aagccatgcc gtcgactgcc
      541 gcaacagttg gaatcatgca atagcgcagc gcctgggaag ttgcctgaaa ctgttcaaca
      601 acttgctcta ctttttccag gcgtccagcc atcaacgaat cggccacgtt caatttgaat
      661 gccgctttta gaatggccga ttgagcgtca cgtttgaatt tcttcatcat ggacgcaaac
      721 gcagtcggct tttgtgcgct ctgccgcgct ccgccgccgg acaaattcgc gccgaccgaa
      781 aacggtggtt cggtctgcca gatgatcagt cccttaaaat tgtgctccga ctcttcgatg
      841 gcttgcatga cgccgtcgag cacgtcctcg ccgatggcat gcatcttggt cttgaaactc
      901 aggatgccga tatcatcgcc ggtggtccac aggcgtgttg catcggtttc aaagactgtc
      961 gtgccgtata ccgaactctc acttatgagc cgatcaggaa acggctggcg gcgatacacc
     1021 ggcaagccgg aacgtggctt gtaagcgcgt tgcgccggac tccaggaacc ctgtgccctg
     1081 tgtacaccat cgcgctgcgg gtcagtagcc cagtccggca atcggaccgc agccatactt
     1141 ttgccggcgg cgatgtcctc cgcaatccag ttcgccatct gttgccatcc ggctgcctgc
     1201 cagagctcta atggaccttg attccagcca aatccccagc ggaccgccag atccagatcc
     1261 cgtgcgttct cggcaattgc ctcgagatga accgcacagt aatgcagcat gtcgcggaag
     1321 gcggaccaaa tgaactgtgc ctgcggatgc tggctcgccc gcaactggtt aaagcgctcc
     1381 gcgacattct tgttcttaag aatggagaca acagactcat cagcttcgcc gccggaaagc
     1441 cggtacgcct gttgtttcag atcgaggact tgtatttcgc ggccctgctt gcgatagatt
     1501 cctcgccctg ttttctggcc tagtgctttc tgatccagca actgcgtgaa ccagtccgga
     1561 atctggtaat acttgtgcca cgggtcgttg ggtaattgct ccttcatcgt attgacgaca
     1621 tgcgcgagcg tatcaaggcc aaccacgtcg gcggtacgat aggtcgcgct tttcggcctt
     1681 ccgatcagcg atccggtcag cgcatcaacc tcgtcgaatc ccaaccccag cctctgtgta
     1741 tgatgaaacg ccgccaggat ggaaaagacg ccaacccggt tggcgatgaa attaggggtg
     1801 tctttagccc gaatcacgcc ctttccaagc gtcgtaacaa ggaattcctc gagccggtcc
     1861 agaatcgcag gatcggtcgc gcgcccggca atcaactcta ccaaatgcat atagcgcggc
     1921 gggttaaaaa aatgcacgcc gcaaaaacgc gagcgcacat gcccgggcat cgcttcggac
     1981 agggaattga tggaaagccc ggacgtattg ctcgcaaata tggcgctttc gccgagataa
     2041 ggagcaactt tctcgtacaa ggtttttttc cagtctatgc gttcggaaat ggcctcgatg
     2101 accaagtcgc agtccttaag tagttccagg tcctgatcgt aatttgccgc ctgtatgtag
     2161 tcggcacgga cgggcacgga gagcggactg ggttccagct ttttcaggct atcaagggcc
     2221 ttgcgtacgt tgccgttggg atcgccctcc ttcgctggca attcaaacag gatcgcttcc
     2281 actccagcgt tcactaagtg ggctgcgatc tgggaaccca ttactccggc gccaaggacg
     2341 gcgactttgc ggacggcaga attgactggg cgtgccatgt tgtctttaga gttgcaacg
//
LOCUS       DATMZQ010000494        18620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_989774, whole genome shotgun sequence.
ACCESSION   DATMZQ010000494 DATMZQ010000000
VERSION     DATMZQ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18620
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989774"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..2159)
                     /locus_tag="VN496_15685"
     CDS             complement(318..2159)
                     /locus_tag="VN496_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXF18450.1"
                     /translation="MTKPSAFVRVPVAAAWRRNDWRTIRTLLPYLMEYKGRVATALLC
                     LTAAKLANVGVPLIMKEIIDSLDAKQAVLALPFALLAIYGLLRLSTTLFAELRDIVFV
                     RVAKRAIRRVALQVFRHLHSLSLRFHLDRQTGGVSRDIERGTRGISTLLSYMLFSIIP
                     VVIEFSLVAFVLLAKFDWRFMAITMGAVVVYLGFTVFVTEWRMEIRRRANELDAKANT
                     RAIDSLLNYETVKYFNNEEFEAKRYDESLQKYEAADVKNEASLGALNIGQSLVIAVAV
                     TALMILAAQGVVAQKLTLGDLVLVNGLLIQLYIPLNFLGMVYREIKQSLVDMDKMFRL
                     LAENREVQDLPDAVALPDRLPTVRFDKVNFSYEERRTILHDVSFEIPAGQTVAVVGAS
                     GSGKSTLARLLFRFYDVTGGRITINGIDLRNLKQTSLRAAIGIVPQDTVLFNDTIYYN
                     IAYGDTHATREQVVEAARSAHIHELISSLPDGYETMVGERGLKLSGGEKQRVAIARAL
                     LKNPKILIFDEATSALDSKSEKAIQTELRRIAISRTTLTIAHRLSTIVDADQILVMES
                     GRIVERGTHRELLARAGTYEHMWTLQQQEERVSELHALENGIAVRGS"
     gene            2359..2796
                     /locus_tag="VN496_15690"
     CDS             2359..2796
                     /locus_tag="VN496_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18451.1"
                     /translation="MGAFVRWLLGLLVVAALLLAAYTWTVLHWSYSAGERAGYVQKLS
                     KKGWICKTWEGELALVTMPGTVGEKFYFSVRDDAMAQQINRSLGRRVSLNYEEHVGLP
                     STCFGETGHFITGVNLIDDPAQLTPAPSHVTPPNPPPASAPKQ"
     gene            2822..3724
                     /locus_tag="VN496_15695"
     CDS             2822..3724
                     /locus_tag="VN496_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXF18452.1"
                     /translation="MLPSSIRVIVRDWLNANHIVLMGRESNVLIDSGYSRDKALTLAR
                     VDEVLAGQPLHWLINTHCHSDHMGGNAAMRAATGCGISVPRDEAPLIQAWDDRALWLS
                     YADQRCDRFSFDTTIAPGEIVEWGNLQWRAIAAPGHDMAALMFFCEEAGILISGDALW
                     QRGFGIVLPGPDRMLRLSATRSTLETIANLRIRLVIPGHGQPFTDIGPALERSFQRLA
                     AFERDESRMVRHVLKVMFVFSLIDHRSMAAAGLDAYLASVPLYAEFNRQYLQLDSIAL
                     TELIVGDLERTGAVRRQDGFLFAA"
     gene            complement(3760..4512)
                     /locus_tag="VN496_15700"
     CDS             complement(3760..4512)
                     /locus_tag="VN496_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXF18453.1"
                     /translation="MEKLQVVTYNIHKGFSQFKRRMSVHDLRERLRGIGADVVFLQEV
                     QGAHETNSVRHSNWPLAPQHEFLADSIWKDFAYGKNAVYDHGHHGNAILSKYPISRWD
                     NIDISMHDSESRGLLHCEMAIPGWRHSLHCVNVHLGLLGHWRKKQISLIKNRIEQLVP
                     ADAPLVIAGDFNDWRRYASDALVKSLEVEEVFETTSGRPARSYPAILPMLHLDRIYVR
                     GFSIELAQAHHGPAWSKVSDHIALSARLVRKV"
     gene            complement(4552..5163)
                     /locus_tag="VN496_15705"
     CDS             complement(4552..5163)
                     /locus_tag="VN496_15705"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXF18454.1"
                     /translation="MTEKLLHAGVLLARQGRRLIVCVISLFSCALSAPGTAAEIHVNT
                     TRHGDTFEVQATAVIQVDVAEAWKVLTDYERLTEFVPGLQESKVVSRNGSNVVLDQSG
                     EAHLLFFKFPMRVRLAITEFPRSRVVSTAIAGNFRQMQGIYRLEAQGKGMQLRYEGSF
                     TPDFDFPPFFGTLLVRSTLEKRFAAMVHEIEKRRRGDSVPGKK"
     gene            complement(5160..6257)
                     /gene="nadA"
                     /locus_tag="VN496_15710"
     CDS             complement(5160..6257)
                     /gene="nadA"
                     /locus_tag="VN496_15710"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXF18455.1"
                     /translation="MAATAIDFSRYSQLQNDDCEARIVAAKKTLGGRVVILGHHYQRA
                     DVYKHADLTGDSLRLSKLSGNTKADYVVFCGVHFMAEVADILGRQGQIVVLPDMAAGC
                     SMADMASLPKVERAWRELSDAVDPEQTVTPVTYINSAADLKAFCGEHGGIVCTSSNAG
                     KVLDWSFKRRQKVLFFPDQHLGRWSGHQMGIPLEQMPIWDPDEPMGGLTMEQIRDAKI
                     LLWKGHCSVHQMFQPQHILKFRNLHPEGIVISHPECKFEVCKLSDYVGSTETIIRTIA
                     AAPANTRWLVGTELNLVNRIAEEFKPQGKHIQFMAPLVCMCSTMARIDPQHLAWTLEN
                     LAQGKVVNQIKVPERDAELARLALERMLSVS"
     gene            complement(6336..6785)
                     /gene="nudB"
                     /locus_tag="VN496_15715"
     CDS             complement(6336..6785)
                     /gene="nudB"
                     /locus_tag="VN496_15715"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HXF18456.1"
                     /translation="MKFKIPVSVLVVIYTPDLQVLLIERADRPGFWQSVTGSQDEGES
                     LTETAIREVFEETGLDARDGVLSDWRLTNEFEIYPEWRWRYAPGIVTNTEHVFGLRLP
                     EAVPARLSPREHVAFLWLPYDRAAEKVFSWSNAKVLRALPELADALR"
     gene            complement(6822..8606)
                     /gene="aspS"
                     /locus_tag="VN496_15720"
     CDS             complement(6822..8606)
                     /gene="aspS"
                     /locus_tag="VN496_15720"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXF18457.1"
                     /translation="MRTEYSGLVCSRHLGQTVSLYGWVHRRRDHGGVIFIDLRDREGL
                     VQIVCDPDNAKTFAIAEKIRNEFVVAVTGLVRPRPAGTVNPNLVSGEVEVLAGSIEIL
                     NTSATPPFQMDDENLSEMVRLEYRYLDLRRPQMQKNLRLRYKVAMAVRRFLDGQGFID
                     IETPMLTKSTPEGARDYLVPSRVHAGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLGVAEITDLMEAMIRSVFRDVLQIELPDPFPRITFAE
                     AMLKYGSDKPDLRVPLQLTELTDAMKTVDFKVFSAVANAANGRVAGLLIPGGGALTRG
                     EIDSYTEFVAIYGARGLAYIKINEAAKGREGLQSPIVKNLSDEALNTIIERSGAKDGD
                     LIFFGADKAKVVNDALGALRVKIGHEKGYADKAWRPLWVVDFPMFDWNEDEKRWEALH
                     HPFTSPMDGHDGLLDSEPGKALAKAYDMVLNGWEIGGGSVRIHRQEIQSKVFSALNIG
                     PDEARQKFGFLLDALQFGAPPHGGLAFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLM
                     CKAPSAVDEKQLRELHIRLRQSDAPKVV"
     gene            complement(8659..9273)
                     /locus_tag="VN496_15725"
     CDS             complement(8659..9273)
                     /locus_tag="VN496_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXF18458.1"
                     /translation="MKKYLITGLLIWVPLVITVWVLSILVGTLDQSLLLLPDDWQPRE
                     WLGFNIPGLGVLLTLGVVILTGVFGANIIGQRMVRVWESLLSRIPVVKSLYSSVKQVS
                     DTLFSSSGLAFRKALLVQYPRQGSWTIAFLTGQPGGDVANHLTGEYLSVYVPTTPNPT
                     SGFFLMMPKADVIELDMNVDEALKYIISMGVVAPEPRAANVRRP"
     gene            complement(9352..9732)
                     /locus_tag="VN496_15730"
     CDS             complement(9352..9732)
                     /locus_tag="VN496_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXF18459.1"
                     /translation="MPIYEYKCTHCGHKKEVLQKLSDPPLRECPACGKAALSKLISAA
                     GFQLKGSGWYATDFKGGAAKAGKDKENQDGKATADGKTEEAGKATTDGKTSEAGKATT
                     GGETAKEGTKSEPAAGSTGASTVQ"
     gene            9874..10626
                     /gene="ugpQ"
                     /locus_tag="VN496_15735"
     CDS             9874..10626
                     /gene="ugpQ"
                     /locus_tag="VN496_15735"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXF18460.1"
                     /translation="MSSQRPFWPYPRIIAHRAGGVLAPENTLAGIRMARNFGYSGVEF
                     DVKLSADGVAVLIHDDSLDRTTNGAGLVEEASYQDIVKLDAGSWFGNEFSGEPVPSFA
                     AASALCRQNGIWANIEIKPSPGRERETGDVVARMTKLLWGGAHPAPLLSSFSALALEA
                     AAVESPDLPRALLVVETPTNWLAQLDRLQCVALHVSHRHVDKQLVNAVHESGRGVLTY
                     TVNDSETALSLLECGVDALVTDQLDTIPPGFA"
     gene            complement(10672..14142)
                     /locus_tag="VN496_15740"
     CDS             complement(10672..14142)
                     /locus_tag="VN496_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXF18461.1"
                     /translation="MTLSSVTLDDKYTLQSGRVFLTGTQALVRLPIMQHLRDRAAGIT
                     TGCYISGYRGSPLGGLDQQLWAAAKHLAAHNIVFQPGVNEDLAATACWGTQQAHIDGD
                     NKYDGVYAMWYGKGPGVDRTGDAFRHGNLAGSAALGGVVCLMGDDHTCESSTTAHQSE
                     FAMVDAMMPILNPAGVQELLDYGLYGWALSRYSGCWVGLKCMKDTIDASASVNVDPAR
                     VRIALPTDFKLPEGGLNIRWPDAPLEQEARLHTYKLEAAKAFALSNGLDQIVMDSPNA
                     KLGIATCGKSYMDVRQALQYLGIDEAEAARLGLRVYKIGMTWPLEPSKATNFAQGLDK
                     IIVVEEKRGLVEPQLKEILYGKPGAPQIVGKLDEAGKILFRSNAALDPNEIAVAIGRR
                     LLARVNDAQLAARVAEQERLQARVLDKPAMDRTPYFCSGCPHSTGTRVPEGSRALAGI
                     GCHFMAQFMDRSTAGYTQMGGEGASWVGEAPFVKTPHMFQNIGDGTYYHSGLLAVRAA
                     AASGVNVTYKILYNDAVAMTGGQHVDGPLTVPQITLQVHGEGAKKIAVVTDEPEKYSS
                     TAEWAPGVTIHHRDDYDQVQREFREIPGLSVIIYDQTCAAEKRRRRKRNQYPDPAKRA
                     FINDLVCEGCGDCGVKSNCVSVLPLETEFGRKRMIDQSACNKDFSCVKGFCPSFVTVH
                     GGGVRKGQSAPAGQNVEALFAAVPEPRLPALQQPYGIMVTGVGGTGVVTIGAILGMAA
                     HLENKGFGALDMAGLAQKGGAVWSHLQIATRPEDIKTVRLGSGGANLVLGCDFVVSAS
                     QKTMDTTREGTRVVVNTHQQMTGEFTRNANMLFPGQSLKRTIIKGVGDQNAEFVDATR
                     IATALLGDSIATNLLMLGYAFQRGLVPVSSEAINKAIELNGAAVKMNQAAFLWGRRAA
                     VDLPAIERLIAPKTPASPAMPLSQTVEELIARRVAFLTNYQDAQYAEKYRKLVERVRQ
                     AELSRTKGLTGLAEAVAHYYFKLLAYKDEYEVARLYADPAFMQNIKAQFEGDYKLHFH
                     LAPPLIAKRDPVSGELRKAEYGSWVFLAFKLLSRLRRLRGTPFDIFGYTHERRMERRL
                     IVEYEATIEQLIGKLSSGNHALAVKIASIPEDIRGYGHVKERSRTIASAKQAELMTQF
                     ESQEVVRSAA"
     gene            complement(14287..14757)
                     /locus_tag="VN496_15745"
     CDS             complement(14287..14757)
                     /locus_tag="VN496_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXF18462.1"
                     /translation="MASVNKVILIGNLGRDPEVRYMPSGDAVANITIATTETWKDKGG
                     EKQEQTEWHRVAMFGKTAEIAGEYLKKGSQVYIEGKLQTRKWTDKEGQERYTTEIRAD
                     RMQMLGSRSGGSERMPPSEEEVPSRSTAAPAKKAPAAAASKGSGFEDLEDDIPF"
     gene            complement(14761..16110)
                     /locus_tag="VN496_15750"
     CDS             complement(14761..16110)
                     /locus_tag="VN496_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF18463.1"
                     /translation="MTHRELRASFGLAGIFGLRMLGMFIILPVFALYAAKLPGGSSHT
                     MVGIALGAYGLTQACLQVPFGWLSDHWGRKRTIYLGLLIFAAGSFIAALAHDIQLIIV
                     GRIIQGAGAISAAVIALAADLTREDQRTKAMAIIGMTIGAAFALSMAAGPVLDRFIGV
                     SGIFALTGFLAIVALFVVRMVVPEPAQISTLKISAPQASLQSVLRDTQLLRLNYGIFA
                     LHGTLMALFVVVPFALQAHLPSGSHWHVYLPVMVGSIVMMFPAMLLAERKSLQKPVFI
                     SAVAVLIGALALLMLAGQDLFPLVIGLLFFFAAFNLLEATLPSMISKLAPAGAKGTAI
                     GVYSSVQFIGTFVGAACGGWLSQHFGAPAVFGFCGTLAVIWLLLAFGMRVPRNFETRW
                     YQLPRADEQMVSGLVHGLSGLPGVREVNLGGDGVAYLKVDSKSFDEQNVIKLLAGET"
     gene            16211..>18620
                     /gene="uvrA"
                     /locus_tag="VN496_15755"
     CDS             16211..>18620
                     /gene="uvrA"
                     /locus_tag="VN496_15755"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXF18464.1"
                     /translation="MDFIRVRGARTHNLKNINLDLPRNRLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARVGEPHCPDHGEPLSAQSVSQMVDHVLQLPEESRLMILAPLVVDRKG
                     ENVDLFEELRAQGFVRVRVDGKVCDLDSVPKLSKTRKHTVEIVIDRLKVRADMQQRLA
                     ESFETALRHADGRALAVEMDNGQEHLFSSRFACPICSYSLSELEPRLFSFNNPMGACP
                     RCDGLGQITFFDPKRVVAFPHMSLAAGAIRGWDKRNQFYFEMIGSLARQYGFDIDTPF
                     EELSEDVRKLILYGTGRDRVRFMYVGERGNKYHREHPFEGILPTLERRYKETDSAAVR
                     EELTKYVASQPCPDCKGTRLRREARNVFVAGKAIFEISALPLKLTQQFFQQVKLSGAK
                     LAISEKIVQEIGKRLQFLINVGLDYLALDRAADTLSGGESQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNDRLLQTLQDLRDLGNSVIVVEHDADAIRSADFVVDMGPGAGVHG
                     GRIVAEGTPAHIAASSESLTGQYLSGTRRIAMPSLRHKPQPNQMLRVVGASGNNLKNA
                     NLELPVGLLVCITGVSGSGKSTLINDTLYNAVAQHLYGSSAEAAPYEKIEGLEFFDKV
                     VNVDQSPIGRTPRSNPATYTGLFTPIRDLFAGVPMARERGYGAGRFSFNVKGGRCEAC
                     QGDGVLKVEMHFLPDIYVTCDVCHGNRYNRETLEVQYKARNIHEVLDMTVEQAREFFS
                     AVPVI"
BASE COUNT         4042 a         5405 c         5011 g         4162 t
ORIGIN      
        1 tggcggactc ctggttcgac tggctcgtcc cccgccatgg cctgaacact ggtcatagcg
       61 cacgcaacac ttagaaaaca aaggattgat ctcatcaagc gcctgaaacc tctatttttt
      121 taattcaatt cgcagttcgg ccttggtttt aaaggccttt atccggtcac tgcccggttt
      181 ttcgagacgg tatgcaacat atcaaaaatc aaggagtaag gaaagacttt agaaaagctg
      241 tttaacttta tcgccgtgag cattcattaa cgacatgttt atagctaacc ccccatgcaa
      301 acagcgagcc acccgcacta tgagcctcgc accgcgattc cgttctcgag cgcgtgcaat
      361 tcgctaaccc gttcctcttg ttgctgtagc gtccacatgt gctcgtaggt acccgcccgg
      421 gcgagtagtt ctctgtgcgt cccgcgttca acgatgcgcc ctgactccat gaccagaatt
      481 tgatcggcat ccactatggt tgaaaggcgg tgggcaatcg tcaatgtggt gcggcttatc
      541 gcaatgcgtc gaagctctgt ctgaatcgct ttttcagact tggaatcaag cgccgatgtc
      601 gcttcatcaa atatcaggat tttcgggttc tttagtaaag cgcgtgcaat agcgactcgc
      661 tgcttttcac cgccagaaag tttaaggccg cgctcgccga ccatggtctc gtatccgtct
      721 ggcagggagc tgatcagctc gtgaatgtgt gctgaacgag ctgcttcgac aacttgctcg
      781 cgtgttgcat gcgtgtcccc gtacgctatg ttgtagtaaa tagtgtcgtt gaacagcact
      841 gtatcttgag gaacaattcc gatcgctgcg cgcaagctgg tttgcttgag attcctgagg
      901 tctatgccgt tgatcgttat cctgcccccg gtaacatcgt agaaacggaa cagcagtcta
      961 gcgagagtgg atttgcccga accactcgct cccacgacag ccactgtctg tcctgctgga
     1021 atctcaaagc tgacatcgtg caagatcgtc cggcgttctt cataactgaa gttcactttg
     1081 tcgaatcgca cggtcggcaa gcgatccggg agagctactg catctggtaa atcctggact
     1141 tcccgatttt ccgcaagcag acggaacatc ttgtccatat caaccaagga ttgcttgatt
     1201 tcgcgataga ccatacccag gaaatttagc ggtatgtaga gctgaatcaa caggccgttg
     1261 accagcacga ggtcgcctag cgtcagtttc tgggccacta cgccctgtgc cgcgaggatc
     1321 atgagcgctg tcacggccac ggcgatgacc agactttgtc cgatattcag ggcgccgagc
     1381 gacgcctcat tcttgacatc ggccgcctcg tatttttgaa ggctttcgtc atagcgctta
     1441 gcttcgaatt cctcattgtt gaagtacttc accgtctcat aattcaggag gctgtctatg
     1501 gcgcgggtat tggctttggc gtcgagttca ttggcgcgac gccggatttc catgcgccac
     1561 tcggtcacaa aaaccgtgaa tcccagatac acgactactg cacccatggt aattgccatg
     1621 aagcgccagt cgaatttggc cagcagcacg aacgcaacca ggctgaattc gatgactacc
     1681 ggaataattg agaacaacat gtaactcagc agcgtagaga tgccgcgagt gccgcgctca
     1741 atatctcgcg acacgccgcc ggtctggcga tccagatgaa agcgcaaact caaactgtgg
     1801 agatgacgaa aaacctgcaa cgccacccga cggattgcgc gcttggcaac tcgcacgaac
     1861 acaatatccc gcagctcggc gaacagcgtt gtggacagtc gcagcaaccc atagatcgcc
     1921 agcagcgcga acggcaatgc aagcacggcc tgtttcgcat ccagcgaatc gatgatttcc
     1981 ttcatgatca gcggcacacc gacgtttgcc aatttcgctg cggtcaggca gagcagcgcc
     2041 gtggccaccc gtcctttgta ttccatcaga tacggcagca gcgtgcgaat ggttcgccag
     2101 tcattacggc gccatgccgc agctacggga acgcgcacga aagcggaagg ttttgtcaaa
     2161 gcgtcttgca acaataaaaa tagatcaatg tagaagtgta atcggtctcg cagatctgcg
     2221 acggctgaaa tccgtcgtgg tgtcgcgacg cgccagcctt tcggagtttt ctatgatccc
     2281 tcgcagctga ttcaatcatc gggctgccaa tgctgttata ttgttgcgcc aatttcccac
     2341 gctggaatgg attcgattat gggcgccttt gttcggtggt tgcttggttt gctggtggtg
     2401 gctgcgttgc tgctggctgc atacacctgg acggtattgc actggagtta ttccgctggc
     2461 gagcgtgcag ggtacgtgca aaaactatcg aagaagggat ggatttgcaa aacctgggaa
     2521 ggggaactgg cattggttac catgcccggc acggtcggcg aaaagtttta tttcagcgtg
     2581 cgcgatgatg ccatggcgca acagatcaac cgttctctcg gtcggcgcgt ttcgctgaac
     2641 tatgaagaac acgtcggact gccatccacc tgctttggag agaccggaca cttcatcacc
     2701 ggtgtcaatc tcatcgatga tccagctcaa ctgaccccgg cgccgtcgca cgtcacgcca
     2761 ccgaatccgc cgcccgcctc tgctccgaaa cagtagcaag ctcttacgct ccttttaact
     2821 catgctgcca tccagcataa gggtaatcgt acgggactgg ctcaatgcca accatattgt
     2881 gctgatgggg cgagaaagca acgttctcat tgacagcggt tacagtcgcg acaaggctct
     2941 aacgctggcc agggtcgatg aggtgctcgc tggacaaccg cttcactggc tgatcaatac
     3001 tcattgccac tcggaccata tgggcggtaa cgccgcgatg cgggccgcaa cgggatgcgg
     3061 catcagcgtt cctcgcgatg aggcgccgct tatccaggca tgggatgacc gggctttgtg
     3121 gttgtcctat gccgatcagc gctgcgatcg tttttctttc gacaccacta tcgccccagg
     3181 cgagattgtt gaatggggaa acttgcagtg gcgtgccatc gcagcacccg gccatgacat
     3241 ggcagcgttg atgtttttct gcgaagaggc cggcatactg atttccggag acgcgctttg
     3301 gcagcgtggc tttgggatag tgctacccgg gccggatcgc atgcttcggc tctcggcaac
     3361 ccgctcaact ttagaaacga tcgcaaattt gcgcatccgc ctggtgatcc ccgggcatgg
     3421 gcaaccgttt accgatatcg gtccggcgct ggagcgtagt tttcagcgtc tggccgcttt
     3481 tgagcgcgac gaatcgcgca tggtgcgcca tgtgctgaaa gtcatgttcg tcttttcgct
     3541 cattgaccac cgcagcatgg ctgctgccgg tctggacgct tatctcgcat cagttccgct
     3601 ttacgctgag ttcaatcgac aatacctgca attggacagc atcgccctca ctgaactgat
     3661 cgttggtgac cttgagcgga ctggcgcagt acgcaggcag gatgggtttc tctttgcggc
     3721 gtgaatggcc ccgcccctgc atgcgccgcg aaccagttct caaaccttcc gaaccagcct
     3781 ggcagacaaa gcaatgtgat cggatacctt ggaccaggca gggccgtgat gcgcttgtgc
     3841 aagctcgata gagaatcccc gcacataaat acggtctaga tgcagcatcg gaagtattgc
     3901 ggggtaactg cgggcggggc gcccggacgt ggtctcaaat acttcctcaa cttccagcga
     3961 tttcactagt gcgtcgcttg catagcggcg ccaatcattg aaatcacccg cgattaccag
     4021 tggcgcatcc gccggcacaa gctgctcaat acgatttttg atcagcgaaa tttgtttttt
     4081 gcgccaatgt ccgagcaggc caagatgcac gttgacgcaa tgtaatgaat gccgccaacc
     4141 tggaattgcc atttcacagt gcagaagacc gcgactttcg ctgtcatgca tggaaatatc
     4201 aatattatcc caacgcgata ttggatattt gctcagaatg gcattgccat ggtgcccatg
     4261 gtcgtacacc gcgttcttgc catatgcaaa atctttccag atcgaatcag ccagaaattc
     4321 atgttgtggt gccaatggcc agttgctatg gcgcacggaa ttcgtctcgt gggcaccttg
     4381 cacctcctgc aggaatacga catctgcgcc gatcccacgt agcctttctc ggagatcgtg
     4441 tacagacatt cgccgcttga attgcgaaaa ccctttgtga atgttgtacg tgacaacctg
     4501 aagtttttcc atttctcgtt cttttttgtc agcaggcgat cagtcagtcc gttatttctt
     4561 gcctggcacc gaatcaccac gacgcctctt ttcaatttca tggaccatcg cggcaaagcg
     4621 tttttccaga gtactgcgca ccaacagcgt gccaaagaat ggcgggaaat caaaatccgg
     4681 cgtaaaactg ccttcatagc gcaactgcat gcccttaccc tgggcttcga gacgatagat
     4741 gccctgcatc tgcctgaaat ttcccgctat tgccgtggat acgacgcgac tacgaggaaa
     4801 ttccgtgatg gctaatcgta cacgcatggg gaatttaaaa aaaagcaggt gggcttcgcc
     4861 gctttgatcg agtacaacgt tggagccatt acgcgataca accttgcttt cttgcagccc
     4921 cggtacgaat tcggtaaggc gctcataatc ggtcagcact ttccatgcct cggccacatc
     4981 aacctggatc actgccgttg cctgaacttc gaaggtatcg ccgtggcgcg tcgtattgac
     5041 atgaatttcg gcagccgtgc cgggcgctga taacgcacac gaaaacaaag aaattacgca
     5101 aactatcaac cggcggccct ggcgggccag cagtacacca gcgtggagaa gcttctccgt
     5161 caagacaccg agagcatccg ttccagcgcc aggcgtgcaa gttccgcatc gcgttccggt
     5221 accttaatct ggttgacaac tttcccttgc gccagatttt ccagcgtcca tgccagatgc
     5281 tgcggatcga tacgcgccat ggtagagcac atgcacacaa gaggcgccat gaactggata
     5341 tgcttgcctt ggggcttgaa ttcctcggcg atgcgattga ccagattgag ctcggtgccg
     5401 accagccaac gagtattggc cggggccgcg gctatggtcc tgatgatagt ctctgtcgac
     5461 ccaacgtagt ctgacagctt gcagacctcg aacttgcatt ccgggtgtga aattacgatg
     5521 ccttccggat gcaggttgcg aaattttaga atgtgctgcg gctgaaacat ctggtgcacc
     5581 gaacagtgcc ccttccacag caggattttg gcgtccctga tctgttccat tgtgagcccg
     5641 cccatcggct catccggatc ccatatcggc atttgctcca gtggaatgcc catctggtgc
     5701 ccactccacc ggcccaggtg ttgatcggga aagaacagca ctttctgacg gcgtttgaaa
     5761 gaccaatcga gaaccttacc ggcattggat gaagtgcaaa cgatgccccc atgctcaccg
     5821 caaaaagcct tgagatcggc cgctgaatta atgtaggtaa ccggagttac ggtttgctcc
     5881 ggatcgaccg catccgacaa ttcgcgccat gcccgctcta ccttcggcag gcttgccatg
     5941 tcggccatgg aacaaccggc cgccatatcg ggcagcacga ctatttggcc ttgtcttccc
     6001 agaatgtcgg caacttccgc catgaaatgc acgccgcaaa acacgacata atctgccttg
     6061 gtatttccgg acagtttcga caagcgcagt gagtcgccgg tcagatcggc atgcttgtag
     6121 acgtctgctc gctggtagtg gtgaccaaga atcacgactc ggccgccaag cgtttttttg
     6181 gctgcaacaa tgcgtgcctc gcagtcgtca ttctgcagct ggctgtagcg gctaaagtca
     6241 attgcggtag cggccatgtc cctgttctct caatcgccaa aaacaactcg gaaaaccata
     6301 ccacaatgag acaaaatccg atgcaaaacg ttgcatcagc ggagcgcgtc cgctagctcc
     6361 ggaagcgccc ggagcacctt tgcattgctc catgaaaaaa ccttctccgc cgcccggtca
     6421 taaggcaacc agaggaaggc aacatgttcc cggggcgaca atcttgccgg caccgcttcc
     6481 ggcaagcgca atccgaaaac atgttcggta ttggtcacaa ttcccggggc gtagcgccag
     6541 cgccattccg gataaatttc gaattcattt gtcagtctcc agtcggacag cacgccgtcg
     6601 cgcgcatcga gccccgtttc ctcgaagact tcgcgtatcg cagtctctgt caggctctcg
     6661 ccttcgtcct ggctacccgt aactgactgc cagaatcccg ggcggtccgc gcgctcaata
     6721 agcaacacct gcaagtccgg cgtgtaaatg actaccagca cagaaaccgg aatcttgaat
     6781 ttcacgttcc tcgcttttat agcccgtatg ttggacttga cctagacaac cttcggtgcg
     6841 tcgctctgtc gcaaccggat atgcaattca cgcagctgct tctcgtccac cgcgcttggc
     6901 gccttacaca tcaggtcctg agcccgttgg gttttcggaa aagcaatcac gtcgcgtata
     6961 gagtctgccc cggccatcat ggttacgatg cgatcgaggc caaaggccaa tccgccatgc
     7021 ggtggcgcgc caaattgaag ggcgtcgagc agaaatccga acttttgcct ggcctcatcg
     7081 ggcccgatat tcagcgcact gaacaccttt gactggatct cctgtcgatg aatgcggacc
     7141 gatccaccgc cgatctccca accgttgagc accatgtcat aggccttggc aagggctttg
     7201 ccgggctcgc tatcgagcaa gccgtcatga ccatccatgg gactcgtgaa cggatgatgc
     7261 aaggcttccc agcgtttttc gtcttcattc cagtcgaaca tcggaaagtc gaccacccac
     7321 aatggacgcc atgctttgtc cgcataaccc ttttcgtgtc cgattttcac ccgtaacgca
     7381 ccgagcgcat cgttgactac cttggctttg tccgcaccaa agaaaatcag atcgccatct
     7441 tttgcgccag aacgctcaat gatggtattc agtgcctcgt ccgaaagatt tttgacgatc
     7501 ggcgactgaa ggccttcccg gcctttggct gcctcattga tcttgatgta cgccaggcct
     7561 ctggcaccgt agatcgccac gaattcggta tagctatcga tctccccccg cgtcagcgcg
     7621 cctccgcctg ggatcaacaa acccgcgacc cggccgttcg ccgcattggc aactgcggag
     7681 aacaccttga aatcgaccgt tttcatcgca tcagtcagtt cagtcaattg cagcggcacg
     7741 cgcaaatcgg gtttgtccga cccatactta agcatggcct cagcaaaagt aatacgcgga
     7801 aacggatccg gcagttcgat ttgcaaaacg tccctgaaga cgctgcggat catggcttcc
     7861 atcaaatccg taatttccgc cacgccgagg aatgaagttt cgatatcgat ctgcgtaaat
     7921 tctggttgtc ggtctgcgcg caggtcttca tcgcggaaac atttggtgat ctggtaatag
     7981 cggtcaaagc cggccaccat cagcaactgc ttgaacaatt gcggtgactg gggcaacgcg
     8041 aagaactcgc ccgcatgcac cctgctcggc acaagatagt cgcgagcgcc ctccggggtg
     8101 gacttggtca gcattggggt ttcgatgtcg atgaaaccct gaccgtcgag aaaacgccgc
     8161 accgccatgg cgaccttgta gcgcaaccgc aaattttttt gcatttgcgg tctgcgcaga
     8221 tcgaggtaac gatattcgag ccgcaccatt tcggaaagat tttcatcgtc catctggaat
     8281 ggtggggtgg ccgaggtatt gagaatctca atggatcctg ctagtacctc gacttctccc
     8341 gagacgaggt tcgggttcac ggtccccgca ggccgtggac gtaccagtcc ggttactgcg
     8401 accacgaact cgttacgtat cttttcggcg atcgcaaaag ttttggcgtt atccgggtcg
     8461 cacacgatct gaaccagacc ttcgcggtcc cgcaggtcga taaagatcac tcctccatga
     8521 tcgcgccgac gatgtaccca gccgtaaagg cttactgtct gaccaagatg tcgggagcag
     8581 accagcccac tatattcagt gcgcataaat tgggatttga agatacgttc gtttgaagat
     8641 gttgctgaag aagaccgatt acggccggcg aacgttggct gctcgaggct ccggagccac
     8701 tacacccatt gagatgatgt acttcaatgc ctcgtccaca ttcatatcga gctcaatcac
     8761 atcggctttc ggcatcatca aaaaaaaccc ggacgttgga ttaggcgtgg tgggaacata
     8821 gacgctcaaa tattccccgg tcagatgatt ggccacatca ccacccggct gccccgtcaa
     8881 aaaagcaatg gtccatgaac cctgacgcgg atattgaacc agcagtgcct tgcgaaacgc
     8941 caggccggaa gatgaaaaca gcgtatccga aacctgcttg acgctggaat aaagtgattt
     9001 aacaaccgga atccggctta acagcgattc ccacaccctg accatgcgct ggccaatgat
     9061 gttcgcgccg aatacgccag tcaggatgac aacgcccaag gtcagcagca cgcctaatcc
     9121 tggaatattg aatccgagcc actcgcgggg ctgccagtcg tccggtagca gaagcagcga
     9181 ctggtccagc gttcccacca ggatgctaag tacccacacc gtgatgacta gtggaaccca
     9241 gatcaataaa cccgtgatga gatatttctt caattgcagt cctgatgtta tccgacaaca
     9301 aggggtacgc aacttatgcg gttgcaccct gttttacgcg agtgagcaat attactgaac
     9361 agtgctcgct ccggtgctgc cggcagctgg ttccgatttg gttccttctt tagccgtttc
     9421 gccgccggtc gttgctttac cggcctccga tgtcttgccg tcggtcgtcg ctttaccggc
     9481 ctcttcagtc ttgccgtcag ccgtagcctt tccgtcctgg ttttccttgt ccttgccggc
     9541 tttggcggcc ccgcccttga agtcggtcgc gtaccagccg cttcctttca attgaaatcc
     9601 cgcggcacta attagcttgc ttaacgctgc tttcccacat gcgggacact cgcgcagagg
     9661 cgggtcagat agtttttgca ggacttcctt cttatgtccg caatgggtgc acttgtattc
     9721 gtaaatgggc atgttatctc tcacaaaaac aaaggaaatt ataccgtatc gaacccgcaa
     9781 ggacgccgag atggaagttc ggaattgcct gctcaagcca tttgccatcg atcgggtggc
     9841 tataatcagc gcaccaaagt cttctagtga ccaatgagta gtcaacgtcc gttctggcct
     9901 tacccccgca tcattgccca tcgcgccggt ggcgtgcttg caccggaaaa tacgctggcc
     9961 ggtattcgaa tggcacgcaa tttcggatac agcggtgtgg aattcgatgt caagttgagt
    10021 gcggatggcg ttgcggtact gatccacgat gacagcttgg accgtacgac caacggagcc
    10081 ggcttggtgg aagaggcgag ttatcaggat atcgtcaagc ttgacgccgg tagctggttt
    10141 ggcaacgaat tcagcggcga acctgtgccc agtttcgcag cagcatcggc cctctgccgc
    10201 caaaacggca tctgggccaa catcgaaatc aagcccagcc ctggtcgcga acgcgagaca
    10261 ggcgatgtgg ttgcccgcat gacgaaatta ctctggggcg gcgcgcatcc ggccccgctg
    10321 ctgtcgtcat tctctgctct ggctttggaa gccgcagcgg tggaatcgcc ggacttgcct
    10381 cgcgctcttt tggtcgtcga aaccccgact aactggcttg cacagctgga caggctgcag
    10441 tgcgttgccc tgcacgtaag ccatcgccat gtcgacaagc aattagtaaa cgccgttcac
    10501 gaatccggac gaggcgtgct gacctatacg gtcaacgatt cggagacagc actcagtttg
    10561 ctggaatgcg gcgtcgatgc gctggtgaca gaccagctgg ataccattcc gcccggtttt
    10621 gcctgacttc tctgtcgtgc accttgctcg tgaagtgctc ttaatcggtc gctatgccgc
    10681 ggaacgtacc acttcctgcg attcaaactg tgtcatcaac tcggcctgtt tcgcggaagc
    10741 aatagtccgg ctgcgttcct tgacatgccc gtaaccgcgg atgtcctccg gaatcgatgc
    10801 gattttgaca gccagggcat gattaccact gctcaatttg ccaatgagct gttctatcgt
    10861 tgcctcgtat tcgactatca agcggcgttc catgcgacgc tcgtgggtat agccaaaaat
    10921 gtcaaacgga gtgccgcgca gacgccgcag cctggacaac agtttgaatg ccagaaatac
    10981 ccagctgccg tattccgcct tccgcaactc gccggaaact ggatcacgct tcgcaatcag
    11041 cggcggcgcc agatgaaaat gaagcttgta gtcgccttcg aattgggctt tgatgttctg
    11101 cataaacgca ggatccgcgt acaagcgcgc cacctcgtac tcgtccttat acgcgagcag
    11161 cttgaagtaa taatgcgcga ccgcttcagc cagcccggta aggcccttgg tccgcgacaa
    11221 ctccgcctgg cgaacccgtt ccaccagttt gcggtacttc tcggcgtact gcgcatcttg
    11281 ataattcgtg aggaacgcga cccgacgggc aattagttcc tcaactgtct gcgacaacgg
    11341 cattgctggc gaagccggcg ttttcggtgc gatgagccgc tctatcgcag gcaggtcgac
    11401 cgccgcgcgg cgaccccaaa gaaaagcggc ctggttcatc tttactgccg cgccattcaa
    11461 ttcgatggcc ttgttgatcg cctccgaaga aaccggcacc aatccgcgct gaaaggcata
    11521 gcccagcatc agaagattgg tggcaatcga atctccaagc agcgcggtgg caatacgcgt
    11581 tgcatccacg aattcggcat tctggtcgcc tacgcccttt attatggttc gcttcagtga
    11641 ctggccggga aacagcatgt tcgcattgcg ggtgaactct ccagtcatct gctgatgcgt
    11701 attgacgacc acgcgcgtgc cctctcgcgt ggtgtccatc gttttctggc tcgccgagac
    11761 aacgaaatcg caacccagca ccagatttgc cccgcccgaa ccgagacgca ccgtcttgat
    11821 gtcttcaggg cgtgtggcga tctgcaggtg actccagaca gcaccccctt tttgtgcaag
    11881 tcccgccatg tccagcgcgc cgaatccctt gttctcgaga tgcgccgcca tgccgaggat
    11941 tgcgccgatc gtcacgacac cggtaccgcc taccccggta accatgatgc cgtacggctg
    12001 ttgcagcgct ggcaggcggg gttcaggaac ggctgcgaac aacgcttcaa cgttctgccc
    12061 ggctggcgcg ctctggccct tgcgtacgcc cccgccatgc acggtgacga aactgggaca
    12121 aaaccctttc acgcaggaaa aatccttgtt gcaggccgac tggtcgatca tgcgtttgcg
    12181 gccgaattca gtttccagcg gcagcacgga tacgcaattc gatttgaccc cgcagtcgcc
    12241 gcaaccctcg cataccagat cattgatgaa ggcgcgcttc gcaggatcgg gatactgatt
    12301 gcgcttgcgg cggcgacgct tttcagcagc acaagtctgg tcatagatga tgaccgacag
    12361 acctggaatc tcgcggaatt cgcgctgcac ttgatcgtag tcgtctcgat gatgaatggt
    12421 cacgcccggc gcccactccg cagtggacga atatttttcc ggctcatcgg tcacgactgc
    12481 aatttttttt gcgccttccc catgcacctg cagcgtgatt tgcggcaccg tgaggggccc
    12541 gtcgacatgc tggcccccgg tcattgcgac cgcatcgttg tagagaatct tgtatgtgac
    12601 attgacaccg gatgcggccg cggcacgaac cgcaagcaag ccggaatggt agtaggtgcc
    12661 atcgccgatg ttctggaaca tgtgcggcgt cttgacaaac ggcgcttcac cgacccagct
    12721 ggcgccctcg ccgcccatct gggtatatcc ggcggtactg cggtccatga actgtgccat
    12781 gaaatgacag ccaattccgg ccagcgcgcg ggagccctct ggcactcgcg taccggtact
    12841 atgcgggcag ccggaacaga aatacggcgt gcggtccatt gccggcttgt ccaatacgcg
    12901 cgcttgcaat ctttcctgtt cagcgacgcg tgcggcaagc tgtgcatcgt ttacccgcgc
    12961 cagcaggcgg cgtcctatgg caaccgcaat ctcgttggga tcaagcgccg cgttggaacg
    13021 gaacaggatc ttgccggctt catcgagctt gccgactatt tgcggcgcac ccggcttgcc
    13081 gtacagaatt tccttcagct gcggctctac caatccgcgc ttttcttcca cgacaatgat
    13141 cttatcaagt ccttgtgcga agttcgtcgc cttgctcggc tcgaggggcc atgtcatgcc
    13201 gatcttgtac acgcgcaggc ccagtcgggc cgcttccgct tcgtcaatgc caaggtactg
    13261 caacgcctgg cgcacatcca tgtacgactt gccgcaggtt gcgatgccaa gcttggcatt
    13321 cggagaatcc atgacaatct ggtccagccc gttgctcaaa gcgaacgcct tcgcggcctc
    13381 aagcttgtac gtgtgcagcc gcgcctcctg ttcgagcggc gcatccggcc agcggatatt
    13441 gagtcctcct tctggcagct tgaaatcggt cgggagagca atgcgcacgc gtgccggatc
    13501 cacattgact gaagcgctcg catcgatcgt gtctttcatg catttaagac cgacccagca
    13561 gcccgaataa cgggacagcg cccagccgta cagaccgtaa tcgagcagtt cctgcacgcc
    13621 ggcgggattc aggatcggca tcattgcatc taccattgca aattcgctct ggtgtgccgt
    13681 cgtggaagac tcgcaggtat ggtcatcacc catgagacaa actaccccgc cgagcgccgc
    13741 agagcccgcc agattgccgt ggcgaaaagc atctccggtc ctatcaacac ccggcccctt
    13801 gccgtaccac atggcatata cgccgtcata cttgttgtcc ccgtcgatgt gggcctgctg
    13861 cgtaccccaa catgccgttg ccgccaaatc ttcgttaacg cctggctgaa agacgatgtt
    13921 gtgcgcagcg aggtgcttgg ctgcagccca cagctgctgg tccaatccgc caagcggcga
    13981 accgcgatag ccggagatgt aacacccggt tgtgatgccc gctgcacggt cgcgcaggtg
    14041 ctgcatgatc ggcaagcgca ccagcgcctg ggtgccagtc aggaagacac ggcccgattg
    14101 cagggtgtat ttgtcgtcga gcgtgacgct ggacaaggtc atggcattgc tttctttaaa
    14161 aattccagac tactgataca gatcggccat cttccgcggc attcgatgac tgttcaatat
    14221 ccttagtatc actgcgcgca ccagaattcc ctcggcgagg gccggtagcc tggcaagaca
    14281 accagttcaa aatgggatgt cgtcttccag atcctcgaac ccactgccct tgctcgctgc
    14341 tgccgcaggc gctttttttg cgggggcagc cgtggaacgg gagggtactt cttcctccga
    14401 cggtggcatc cgttccgaac cgccagaacg tgaaccaagc atttgcatgc ggtccgcacg
    14461 gatttccgtc gtgtagcgtt cctggccttc cttgtcggtc catttgcgcg tctgcagctt
    14521 gccttcaatg tagacttgcg agcccttttt caagtattcg ccggcaattt cggcagtttt
    14581 gccaaacatg gccacccgat gccattccgt ctgttcctgt ttctcgccgc ccttgtcttt
    14641 ccacgtttca gtggtcgcaa tggtgatatt ggccaccgcg tcgccgctgg gcatgtagcg
    14701 cacctcggga tcacgcccga gattgccgat caggatcact ttatttaccg aagccatcgc
    14761 tcaagtctcc cctgccagta gtttgatgac attttgttcg tcgaagctct tactgtccac
    14821 tttaaggtat gccactccgt cgccgccgag gttcacttca cgcacgcctg gcagcccaga
    14881 caggccgtgc accagtccgg agaccatctg ctcatcggca cggggcagct gataccagcg
    14941 tgtctcgaaa ttccgcggca cccgcatacc gaaagccagc aacaaccaga tgaccgccag
    15001 tgtgccgcaa aaaccgaata cggccggtgc accgaaatgc tgagacagcc agccgccgca
    15061 cgcggcgccc acaaatgtgc cgataaattg cactgagctg tagacgccta tggccgtgcc
    15121 cttcgcaccg gcaggagcaa gcttggagat catagatggc agcgttgcct ccagcaaatt
    15181 gaaggccgcg aagaaaaaca gcagaccaat cacaagcggg aacaggtctt gacccgcgag
    15241 catcaataag gcaagcgcac caatcagtac cgctacggca gagatgaata ccggcttttg
    15301 cagcgattta cgctcggcga gcagcattgc cgggaacatc atcacgattg atcccaccat
    15361 gacaggcaga tagacatgcc aatgactgcc agaaggcaga tgcgcctgca gcgcgaatgg
    15421 caccaccacg aacagagcca tcagggttcc gtgcagtgcg aatatgccgt agttgagccg
    15481 cagcagctgc gtgtcacgca agacggattg gagcgatgcc tggggagcag atattttcag
    15541 cgttgaaatt tgcgcgggtt ccggcaccac catgcgcacg acgaacagcg ccacaatggc
    15601 cagaaagccg gttaacgcaa atatccccga gacaccgata aagcgatcca gcaccgggcc
    15661 cgcggccatc gatagcgcga acgctgcccc aattgtcatg ccaatgatcg ccatggcttt
    15721 ggtgcgttgg tcttcccgcg ttaaatccgc agccagtgcg atcacggcag cggaaatggc
    15781 gccagcgccc tggatgattc gacccacgat gatcaactgt atgtcgtgtg cgagcgcggc
    15841 gatgaagcta cccgctgcga aaatcagcaa gccaaggtag atcgtgcgtt tgcgccccca
    15901 gtggtcagac aaccagccga aaggcacttg cagacaggcc tgcgtgagcc cgtaggctcc
    15961 gagcgcgatt ccaaccatcg tatggctgct gccgccggga agcttcgcgg cgtaaagcgc
    16021 gaacacgggc aggatgataa acatgcccag catgcgcagt ccaaaaatgc cggccagacc
    16081 aaagctggcg cgcaactcgc ggtgcgtcat gcggtctgat gcaggcattc tggactgtgc
    16141 tgaaacagcg gttgggaaac gcttttgaag tgcagtatat tagcaggttg accaaatact
    16201 cattcatcga atggatttca ttcgcgtgcg cggcgcacgc acgcacaatc tgaagaacat
    16261 caatctcgat ctgccacgca accggttaat cgtgattact ggcttgtcgg gttcaggcaa
    16321 gtcctcgctt gctttcgata cgctgtacgc ggaaggccaa cgccgctatg tcgaatcgct
    16381 gtcggcttac gcgagacagt ttctgcagct gatggaaaag ccggacgtgg atttgatcga
    16441 aggtctctca ccggccattt cgattgaaca gaaagccacc agccataatc cccgctccac
    16501 ggttggcacc gtcacagaga tccacgatta ccttcgcctg ctgtatgccc gcgttgggga
    16561 acctcactgc cccgatcatg gcgagccgtt gtcggcacaa agcgtttcgc agatggtgga
    16621 ccacgtgctg cagcttccgg aagaatcgcg gctcatgatt cttgcgccgc tggtggtgga
    16681 ccgcaaaggt gaaaatgtgg acctgttcga ggagctgcgc gctcagggct ttgtgcgggt
    16741 tcgcgtcgac gggaaagttt gcgatctcga cagtgtgccg aaactttcca agaccaggaa
    16801 gcacaccgtc gaaattgtca tcgatcggct taaagtgcgc gctgacatgc aacagcgcct
    16861 cgccgaatcg ttcgagacag cactgcgcca cgccgacggg cgcgcattgg cggtggaaat
    16921 ggataacggg caggaacatc tgttttcctc aagatttgcg tgcccgattt gcagctactc
    16981 gctgtctgaa ctcgagccgc gactgttttc gttcaacaac ccgatggggg cttgcccgcg
    17041 ctgcgatggc ctgggacaaa tcactttttt cgacccgaaa cgcgttgttg catttccaca
    17101 tatgtcgctg gctgctggcg caatcagagg ctgggacaag cgcaatcagt tctacttcga
    17161 aatgatcggc agccttgcgc ggcagtacgg tttcgacatt gacacgcctt tcgaagaact
    17221 gtccgaggac gtacgcaagt tgattctgta tggcaccggc cgcgacaggg tgcgcttcat
    17281 gtatgtcggc gagcgcggca acaaatatca tcgcgagcat ccgtttgaag gtattttgcc
    17341 gacactggaa aggcgatata aggaaaccga ttcggccgcc gtacgcgaag agctgaccaa
    17401 atatgtcgcg agccagccat gtcccgactg caagggcacc cggctgcggc gggaggcgcg
    17461 caatgtattc gttgccggca aggcgatctt cgaaatcagc gcattgccgc tgaaactgac
    17521 tcagcagttt tttcagcagg ttaaattgtc cggcgcgaag ctcgcaattt cggaaaagat
    17581 cgtgcaggag ataggcaagc ggctgcaatt cctgattaac gtcggcctgg actatcttgc
    17641 gcttgaccgc gcagcggaca cgctttccgg cggcgagtcg cagcgtatcc ggttggcctc
    17701 gcagattggc tcggggctga cgggtgtgat gtacgtgctc gatgagcctt cgatcggtct
    17761 tcaccagcga gacaacgatc gtttgctgca aaccctgcag gatttaagag acctcggcaa
    17821 ttcggtcatt gtggttgaac atgatgccga tgccatccgc agtgccgatt ttgtcgtcga
    17881 catgggcccg ggtgccggcg tgcacggcgg gcgcattgtc gctgaaggaa cgccagctca
    17941 tatcgcggca agttcggaat cgcttaccgg acaatacttg tccggcaccc gccgtattgc
    18001 catgccgtcc ttgcgccaca agcctcagcc gaaccagatg ctgcgagtgg tgggggcaag
    18061 cggcaataat ttaaaaaatg cgaacctgga actcccggtg gggttgctgg tttgcataac
    18121 gggcgtttcc ggatccggca aatccacact tatcaacgac accctctata acgcggtggc
    18181 gcagcatctg tatggcagca gcgcagaagc ggccccatac gaaaaaatcg agggactgga
    18241 atttttcgac aaagtcgtta acgtggacca gagtccgatc ggtcgaacgc cgcgttccaa
    18301 cccggccacg tataccggcc tgttcacacc aatccgcgat ctgttcgcag gcgtgccaat
    18361 ggcgcgcgag cgcggttacg gagcgggccg tttttccttt aatgtcaaag gggggcggtg
    18421 cgaagcttgc cagggcgatg gcgtgctgaa agtggagatg catttcttgc cggacatcta
    18481 tgtaacctgc gacgtttgtc acggcaatcg ctacaaccgt gagactttgg aagttcagta
    18541 taaagcccgc aatattcacg aggtacttga catgaccgtg gagcaggcgc gggagttttt
    18601 cagcgctgtt cccgtcattt
//
LOCUS       DATMZQ010000495         4358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4792591, whole genome shotgun sequence.
ACCESSION   DATMZQ010000495 DATMZQ010000000
VERSION     DATMZQ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4792591"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VN496_15760"
     CDS             complement(<1..453)
                     /locus_tag="VN496_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF18465.1"
                     /translation="MYYLKFIVRNALRHKLRTALTMFGIFVAILAFGLLRTVVESWFA
                     GSENASATRLVTRNAISLVFPLPITYGDRIRQVEGVAHVSHANWFGGIYVSERNFFPQ
                     FAVEASTYLPLYPEYRMAPEDVRAFLLDRKGCVVGRKLARQYGFKVGDT"
     gene            complement(500..1198)
                     /locus_tag="VN496_15765"
     CDS             complement(500..1198)
                     /locus_tag="VN496_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18466.1"
                     /translation="MTGSPIVQIRHLYKSYRRGGQVVPVLNDLSFDIPEGEFIALMGP
                     SGSGKSTLLNLIAGIDKPDSGDLLIDGEDISQLSESALADWRAANIGFIFQFYNLIPV
                     LTAFENIELPLLITGLGRRDRRARVQLALDLVSLTDRSLHYPSELSGGQQQRVAIARA
                     IITDPLLIVADEPTGDLDRVSAGDILGLLERLNREIGKTIVMVTHDQRAAERARVIMQ
                     LEKGELASRETATV"
     gene            complement(1199..2425)
                     /locus_tag="VN496_15770"
     CDS             complement(1199..2425)
                     /locus_tag="VN496_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF18467.1"
                     /translation="MDTPDLSRLKINRAKGASTRHDQSRWRRHTVAASLVLLALIAAA
                     AIYYRMTTAALEVETVSVTLAYPSQSFTVLNATGYVVAQRKAAVASKATGRIIWLGVQ
                     EGSRVRKDDIIARLEDLDVRAAREQAAANVQVARANLTQSQAELKDARNALERSQGLL
                     QQGFVSQASHDIAVARFDKATAQIASQQAAINAALANLRGAQVAVEQTIIRAPFDGVV
                     LTKAANVGDIVTPFSSALDTKGAVVTMADMTTLEVEADVSESNLSKVRIGQPVEIQLD
                     ALPDKRFRGEVARTVPTVDRSKATVMTKIRFLDPDPRVLPEMSAKASFLSREVSDAER
                     TARPAVNPGAIVKRGGSDVVFVVKDGKLVQMPVETGPTLGDLVELRKGPQPGEKVVLR
                     PLDKFHDGATVKLVAK"
     gene            complement(2581..3783)
                     /locus_tag="VN496_15775"
     CDS             complement(2581..3783)
                     /locus_tag="VN496_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXF18468.1"
                     /translation="MENLKVSSDALFILLGAVMVLAMHGGFAFLELGTVRRKNQVNAL
                     VKILVDFSVSTVAYFFIGYTLAYGVNFFSAADTLAAGSGYQLVRFFFLLTFAAAIPAI
                     VSGGIAERAKFNTQLAATFLLVAFIYPFYEGIAWNGNFGIQGLLEKSFGAPFHDFAGS
                     IVVHAVGGWVGLVAVLMLGARRGRYSKEGGIAAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQSIDKISGLVAVNSLMAMAGGTIGALIAGRNDPGFVHNGPLAGLVAICAGSDLMH
                     PLGGLATGLIAGFLFVFMFTVTQNRWKIDDVLGVWPLHGLCGAWGGIAAGIFGLKAFG
                     GLGGVSLLAQITGSLLGISVAVIGALIMYGTLKAFVGLRLDPEQEFMGADLSIHKISS
                     TAEKEVSW"
     gene            4049..>4358
                     /locus_tag="VN496_15780"
     CDS             4049..>4358
                     /locus_tag="VN496_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18469.1"
                     /translation="MAANTPQPVAKKQSPGTGASDPAAPDKRSQKLPDHKLTLDELLG
                     WLQADKLVASDIAEKLLSLRKNFRASNNHPLTLLADQKWQDPRSPKKLLTLEALTEWL
                     A"
BASE COUNT          990 a         1338 c         1172 g          858 t
ORIGIN      
        1 ggtatcgcct actttgaatc cgtactgccg cgccagtttg cgaccgacca cgcaaccctt
       61 tcgatcgagc aggaacgccc ggacgtcttc aggcgccatg cgatattccg gatagagcgg
      121 cagatatgtg gacgcctcga ccgcaaattg cggaaagaaa ttacgttccg agacgtaaat
      181 tccgccgaac cagttggcat ggctgacgtg agcgacccct tcgacctgcc ggatgcgatc
      241 gccataagta atcggcagcg ggaacaccag cgaaatcgcg ttgcgggtca ccagccgggt
      301 ggctgacgca ttttccgatc ctgcgaacca ggattccacc actgtgcgca acaatccaaa
      361 tgccagaatg gcaacgaaaa tgccgaacat ggtgagcgcg gtccgcagtt tgtggcgcaa
      421 ggcattgcgc acgatgaact tcaagtaata cataaacgtc gtcgccagct aaaacaatta
      481 agcgtgcccc atacggcctt cagaccgttg ctgtctcgcg gctggcgagc tcaccttttt
      541 ccagttgcat gatcacgcgc gcccgctcgg ctgcgcgctg gtcatgcgtg accatcacga
      601 tggtcttgcc gatctcgcga ttcaggcgtt ccagcaatcc cagaatatcc ccggcggata
      661 cgcgatccag atcgccggtc ggttcatcgg cgacgatcag cagcggatcc gtgatgatcg
      721 cgcgtgcgat tgccacgcgc tgctgctggc cgccggacag ttccgacggg taatgcaagg
      781 aacggtcagt aaggctcacc agatccagcg cgagttggac ccgcgcgcgc cggtcacgcc
      841 ggcccaatcc ggtaatcagc agtggcagct cgatgttctc gaaagcggtt aataccggaa
      901 tcagattgta gaactgaaaa atgaaaccga tgttcgccgc gcgccaatcg gccaaagcgg
      961 actccgaaag ctgcgaaata tcttctccgt caatcagcag gtctccgcta tcgggtttgt
     1021 caatgccggc aatcagattg agcaatgtgc tcttgcccga accagaagga cccatcaggg
     1081 caataaattc gccttccggg atgtcgaagc tcagatcgtt caataccggg acgacctgac
     1141 cgccgcgtcg atacgacttg tacagatggc gtatctgaac aatgggagag cctgtcattt
     1201 acttggctac caacttgacg gtcgcgccat catggaattt atccagtggc cgcagcacca
     1261 ctttttcacc gggctgcggc cctttgcgca attccaccag atcgccgagc gtgggcccgg
     1321 tttccacagg catttgcacc agtttgccgt ccttgaccac aaagacgacg tcgctgccgc
     1381 cgcgcttgac gatcgctcct ggattcaccg ctggccttgc tgtgcgttcg gcgtcgctga
     1441 cttccctgga gagaaacgag gccttcgcgc tcatttcagg caataccctc gggtcaggat
     1501 ccagaaaacg gattttggtc attaccgttg ccttggagcg atcgaccgtg ggaacggtgc
     1561 gggcaacctc gccgcgaaag cgtttgtctg gaagggcgtc gagctgaatc tccacgggtt
     1621 ggccaatgcg aactttggac agattcgatt cggacacatc agcttccact tccagagttg
     1681 tcatgtccgc catggtgacc accgcgccct tggtgtccaa tgccgatgaa aacggcgtga
     1741 cgatatcccc gacgttggct gccttggtca gcaccacgcc atcgaacggt gcgcgaatta
     1801 ttgtttgctc cacggcgacc tgagcgccgc gcagattcgc cagcgcggca ttgatggcgg
     1861 cctgctggct ggcaatctgc gcggtcgcct tatcgaatcg cgcgacggca atatcgtggg
     1921 aggcttgcga cacgaagcct tgttgcaaca agccctgcga tcgctccagt gcattgcggg
     1981 cgtctttcag ttccgcttgc gactgcgtca aattggctcg cgcgacctgg acattggccg
     2041 ctgcctgctc acgcgcagcc cgcacgtcaa ggtcttcgag ccttgcgata atgtcatcct
     2101 tgcgcacacg gctgccctcc tgcacgccga gccagatgat gcgcccggtt gccttcgaag
     2161 ccaccgcggc cttgcgctgg gccacgacat agcccgtcgc attcaacacg gtgaagcttt
     2221 gcgagggata tgccaacgtc acgctgactg tttccacttc gagcgcagct gtggtcatgc
     2281 ggtaataaat ggctgcggcg gcaatcaggg cgagaagaac cagggacgcc gcaaccgtgt
     2341 gtcgcctcca gcggctttga tcatgccggg tcgacgcacc cttcgcgcga ttgattttaa
     2401 gtctcgagag atctggcgta tccaagcaag ttacctgaac agtggtcgct tcattatgac
     2461 cgaagctgcg gggtgtttgg accaatcggc cttgagcagg tgcgcacgaa gcacaaaaaa
     2521 taaagcaggc ggcgtctcga cgccgcctgt tgctgtacgc atctgctcaa tcgtgcgcaa
     2581 ctaccagctg acctcctttt cggcagtcga cgaaatctta tggatactca gatcggcccc
     2641 catgaactct tgctccggat ccaggcgtaa gcccacgaat gcctttaatg tgccgtacat
     2701 gatcagtgct ccgatcaccg ctaccgaaat accgagcaac gaacctgtaa tctgcgccag
     2761 caggcttacg cccccgagtc ctccgaatgc tttcaacccg aagatgccgg ccgcgatacc
     2821 accccacgcg ccgcaaagcc cgtgcaacgg ccacaccccg aggacatcgt cgatcttcca
     2881 gcgattttgc gtgacggtga acatgaaaac gaacaagaat ccggcaatca atccggtcgc
     2941 cagtccgccc agaggatgca tgaggtcgga cccggcgcag atcgcgacca gtcccgcgag
     3001 tggtccatta tggacaaagc cgggatcgtt gcggccggca atcagggcgc caatggttcc
     3061 ccccgccatc gccatcaacg agtttaccgc cacaaggccg ctgatcttgt cgatggactg
     3121 cgccgacatg acgttgaagc caaaccagcc aaccgtgaga atccacgctc ccagcgccag
     3181 aaacggaatg ctggagggcg gatgtgcggc aatcccgcct tccttgctgt aacgcccgcg
     3241 gcgtgcaccc agcatcagaa ccgccaccag accaacccag ccaccaaccg catgcacgac
     3301 aatggaaccc gcaaaatcat gaaacggcgc accgaaagac ttctccagca agccctggat
     3361 accaaagttg ccgttccagg cgatcccttc atagaacggg tagataaagg ccaccagcag
     3421 aaatgtcgcc gccagctgtg tattaaattt ggcgcgttca gcgattccgc ccgaaacgat
     3481 cgcaggaatg gcggccgcaa aggtcagcag aaaaaagaat cgcacaagct gatatccact
     3541 gcctgccgca agcgtgtccg cggcagagaa aaagttgact ccgtaggcca gcgtgtaacc
     3601 gatgaagaag tacgcaaccg tggaaacaga aaagtcgacc aggatcttca ctagcgcgtt
     3661 gacctggttt ttcctgcgca ctgtgcccaa ttcaagaaat gcaaatcccc catgcattgc
     3721 caggaccatg accgctccga gcaaaatgaa tagagcatca ctgctcactt tcaaattttc
     3781 catgcttttc cccctgtttt tttatttgcc aaacgttgtt tccggaacgg aaatccctac
     3841 tccaaaggca caagaacctg taatgtttcc acttcacagt tccgttctgg gaagttacct
     3901 gaaaaatcgc ccgttatggt gcccactgcc cggttggtgc accatgtcga aaagtccgaa
     3961 ccgcgtcctg acccggctcg ccgcagccag cctggggggc aggagcctca gctttctttt
     4021 atagtggcgt agggattcct aagaaaacat ggccgccaat acacctcaac ccgttgcaaa
     4081 gaagcagtcc ccaggcaccg gcgcaagcga cccggcagcg ccggacaagc gcagccaaaa
     4141 gctcccggac cataaactca cgctcgacga attgctcggt tggctgcaag ccgacaagct
     4201 tgtcgcgtcc gacatcgcgg aaaaactgct ttccttgcgg aagaattttc gggccagcaa
     4261 caatcatccg ctgactcttc tggcggatca aaaatggcag gacccgcgca gcccgaagaa
     4321 attattgacc ctcgaggcgc ttaccgaatg gctggcgg
//
LOCUS       DATMZQ010000496         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4445464, whole genome shotgun sequence.
ACCESSION   DATMZQ010000496 DATMZQ010000000
VERSION     DATMZQ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4445464"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VN496_15785"
     CDS             complement(<1..572)
                     /locus_tag="VN496_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXF18470.1"
                     /translation="MHRKPLDSVALSTMVLCCFVWGFTNVVIKFAASDISPVMQGGLR
                     SVLATALIVSWARMRHIPLFQSDGTFRAGLVAGALFAGEFLFVYAGLAHTGASRMVVF
                     LYTAPCLTALGLHWCVPGERLHSVQWFGVLLAFGGIVAAFGDGFASSPASLLGDAFGV
                     LGGIFWAATTVWIRATGLTRISAAKVLFYQL"
     gene            749..1453
                     /gene="pcaH"
                     /locus_tag="VN496_15790"
     CDS             749..1453
                     /gene="pcaH"
                     /locus_tag="VN496_15790"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HXF18471.1"
                     /translation="MVDVAGYRRPPKGTQPEYLHAPYASTRKRAPTRPLVLLPHTLTE
                     ITGPLFGHDAVRPEDADLTKQHAAAPVGERIIVSGRVLDEHERPVPDTLVEIWQCNAA
                     GRYRHQMDNHDAPLDPNFTGAGRVLTGRDGEYRFITIKPGAYPWRNHHNAWRPAHIHL
                     SLFGRAFATRLVTQMYFPGDPLLPHDPIFNSTANERARYRLVSDFDLDTTVPEHALGY
                     RFDIVLRGRNATPLDK"
     gene            1431..2015
                     /gene="pcaG"
                     /locus_tag="VN496_15795"
     CDS             1431..2015
                     /gene="pcaG"
                     /locus_tag="VN496_15795"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HXF18472.1"
                     /translation="MRPRWINETHLTATASQTVGPYFRIGLEALYISDLAPSAQPQQK
                     IVIEGRVLDGDGQPVNDAILEIWQANEHGRYAHPDDTQNKPLTQGFTGFGRVPVDQNG
                     AFRFSTIKPGSVTGPRGMPQAPHLLVAVFMRGLLIHLITRIYFPGDPGNADDPVLQLV
                     PKDRRSTLVARQSADGLQWNVILQGEGETVFFEC"
     gene            2080..>2729
                     /locus_tag="VN496_15800"
     CDS             2080..>2729
                     /locus_tag="VN496_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HXF18473.1"
                     /translation="MVAIFSDHGRLQGMLDFEAALARAQARAGVIPNSAAPPIGTQCL
                     ANLFDIDALARAASLAGNPAIPMVKALTQRVAEIDQQAAGFVHWGATSQDAMDTGLVL
                     QLRSALALIDDDVARLSDALAHLALQHGKTSMPGRTWLQHAVPITFGLKIAGALSAVE
                     RHRMRLRELRARVLVIQFGGAAGTLASLGAQGIEVCNALATDLKLANPDMPWHAHR"
BASE COUNT          575 a          869 c          809 g          476 t
ORIGIN      
        1 agttgataaa acagcacctt tgccgcactg atgcgtgtca gaccagtagc acgaatccaa
       61 acggtggtcg ctgcccagaa aatcccgccc aggacgccga atgcatcgcc cagcagcgac
      121 gcaggcgatg acgcgaagcc atctccaaaa gcggcgacga taccgccgaa cgccagcagg
      181 acgccaaacc actgcaccga gtgcagtcgc tcccccggta cgcaccagtg caagccgagc
      241 gccgtcaggc acggtgccgt atagaggaac accaccatgc gggatgcgcc cgtgtgtgca
      301 agcccggcat agacaaacag gaattctcct gcgaacagcg ccccggcaac cagtccggct
      361 cgaaaggtac cgtcgctctg gaacaaggga atgtggcgca tgcgtgccca cgacacgatc
      421 aatgcagtcg cgagtacgga acgcaggcca ccttgcatga ccggagagat gtcgctggca
      481 gcaaatttga tcacaacatt ggtgaagccc cacacgaagc agcacaacac catcgtcgac
      541 aacgcaaccg agtcaagcgg tttacgatgc attcaaatcc aggaaaacgg caaaggcgct
      601 agggtactgg attgttcgca taacactgcc gcgatttggt gactacgggg ctctgcaaag
      661 gcgtgcaaac cataatggcg cgttctgaga caattaccat ccagtcggcc ggcgcgctgg
      721 tcagggccgg taaggggaaa gggaacacat ggttgatgtt gccggttacc ggcggccccc
      781 caaggggacg cagcccgaat atttgcatgc accttacgcg tccacgcgca agcgagcgcc
      841 gacgcgcccg ctggtgttgt taccgcatac cttgacggaa atcaccgggc cgttgttcgg
      901 acacgacgcc gtccgcccgg aagatgctga tctcacgaag cagcacgcag cggcgcccgt
      961 gggcgaacgt atcattgtga gcggacgtgt gctggacgaa catgagcgcc cggtaccgga
     1021 cacgctggtg gagatctggc aatgcaatgc cgccggccgt taccggcatc agatggacaa
     1081 ccacgatgct ccgctggatc ccaactttac cggcgcgggt cgcgtgctta ccggccggga
     1141 cggagaatac cgtttcatca ccatcaaacc cggggcatac ccatggcgca accaccacaa
     1201 cgcctggcgc ccggcccaca tccatctgtc cctgttcggc cgcgcattcg ccacgcggct
     1261 ggtaacccag atgtattttc ccggcgatcc attgctgccg cacgatccga ttttcaacag
     1321 cacggccaat gaaagggcgc gttaccgcct ggtgtccgat ttcgacctgg acaccacggt
     1381 acccgagcat gcgctgggtt atcgtttcga catcgtattg cgcgggcgca atgcgacccc
     1441 gctggataaa tgaaacgcac ctgaccgcga ctgcatcgca aaccgttggt ccctacttcc
     1501 gcatcggact cgaggcgctc tacatcagcg atctcgcgcc gtccgcgcag ccgcaacaga
     1561 aaatagtaat cgagggccgc gtcctggatg gcgacggcca gcccgtcaat gacgctattc
     1621 ttgaaatctg gcaagcgaat gagcacggaa gatatgcgca tcccgacgac acgcagaaca
     1681 agccgctgac gcaaggcttc accggtttcg gccgcgtgcc ggtcgatcaa aacggcgcat
     1741 tccgcttcag caccatcaag ccgggaagcg tgaccgggcc gcgcggcatg ccgcaggcgc
     1801 cgcatttgct cgtcgcggtg ttcatgcgtg gcctgctgat tcatttgatc acgcgcattt
     1861 acttccctgg cgatccgggg aacgccgacg atccagtgct tcaactcgtc cccaaggatc
     1921 gccgttccac gctcgtcgca cgtcaatccg cagatggcct gcagtggaac gtaattctgc
     1981 aaggtgaagg cgagactgtt tttttcgaat gctgacctgc aagctggccc cttacaaaac
     2041 gtgacgcgtt acctggatcc attgtttcgg accgacgcca tggtcgcgat tttttccgac
     2101 cacggccggc tgcagggcat gctcgatttc gaagccgctt tggcacgcgc gcaggcgcgc
     2161 gcgggcgtga tccccaacag cgccgcgccc ccgatcggca cgcaatgcct ggcgaacctg
     2221 ttcgacatcg acgcgctggc acgcgccgcg tcgcttgccg gcaatccggc catccccatg
     2281 gtgaaggccc tgacgcaacg ggttgccgaa attgatcaac aggccgcagg cttcgttcac
     2341 tggggcgcga ccagccagga cgcgatggat accggcctgg tgctgcagtt gcgcagtgcg
     2401 ctggcgctga tcgatgacga cgtcgcgcgc ctgtctgatg cgttagccca tctcgcgctt
     2461 caacatggca agacttcgat gcctgggcgc acctggctgc agcacgcggt accgattacc
     2521 tttgggctga aaatcgcggg cgcgttgagc gcggtggaac gacatcgaat gcggctgcgt
     2581 gagctgcgcg cccgcgtgct ggtaatccag ttcggaggcg ccgccggaac gctggcttcc
     2641 ctcggtgcgc aggggattga ggtttgcaac gcactggcca ccgacttaaa gctcgcgaat
     2701 ccggacatgc cgtggcatgc gcatcgtga
//
LOCUS       DATMZQ010000497         3557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3757496, whole genome shotgun sequence.
ACCESSION   DATMZQ010000497 DATMZQ010000000
VERSION     DATMZQ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3757496"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VN496_15805"
     CDS             complement(<1..548)
                     /locus_tag="VN496_15805"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HXF18474.1"
                     /translation="MPHAFAPVFLARQIRTIEHSLLSQPAAPPLMARAGLAVAEVARD
                     MLGDRGKRVLVLAGPGNNGGDAFVAALHLKQWWYEVAVLFTGSEANLPPDAHLAFKQW
                     HAAGGTTHVSIPASTGWQLIVDGLFGIGLERPLDGHYSDLVNWSNGSGIPILAIDIPS
                     GLHADSGRILGHAMHANRTVTF"
     gene            608..>3557
                     /gene="purL"
                     /locus_tag="VN496_15810"
                     /gene_synonym="purI"
     CDS             608..>3557
                     /gene="purL"
                     /locus_tag="VN496_15810"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HXF18475.1"
                     /translation="MPDFLTLRGRNALSEFRLNKLTQALKKSVPAISGIATEYWHFVQ
                     LKAPLQPPQRRILDRLLTYGPAPAQVSESGRLLLVVPRLGTISPWASKATDIAHSCGL
                     DVVERIERGLAYYFTTSLGKSLNRAQQRAVLPLIHDRMTQSVLDSLDEAKQLFQHFPA
                     TPLTTIDVTRGGRKALERANVEMGLALSDEEIEYLMEYFRRVARDPTDVELMMFAQAN
                     SEHCRHKIFNAQWIIDGSTKDKSLFSMIRHTHAAHPAGTVVAYADNASVIEGAEIERF
                     YPDASGSYRYSTEQTNILMKVETHNHPTAIAPFEGAATGSGGEIRDEGATGRGSKPKA
                     GLTGYSVSHLRIAGFEQPWEKRNIGKPERIASALQIMLEAPIGAASFNNEFGRPNLCG
                     YFRTFEHAVASEVRGYHKPIMLAGGVGSIPALQSFKSGVPADSLLVQIGGPGMLIGMG
                     GGAASSMNTGANIADLDFDSVQRGNAELQRRAQEVIDRCWALGTDNPVLSIHDVGAGG
                     LSNALPELVYGAGRGGDIDLRDIPSEEPGMTPAQIWCNEAQERYVLAIDEERLADFKA
                     LCERERCPFAIVGRATADGILKVRDPQFNNDPVDMPLEVVLGKPPKMVRDVRRRIRVL
                     PPADFSMVDVADAVSRVLRFPAVANKTFLISIGDRTVGGLCSRDPFVGPWQVPVADVA
                     VTLMGFATYRGEAFAIGERTPIAVLNGPASGRMAVGEALTNIAAAPVEKLGDVKLSAN
                     WMAAAGYPGEDAILFDTVEAVAMELCPALGISIPVGKDSMSMRTAWQDGHEKKQVVAP
                     LSLIVSAFAPCVDARHTLTPQLQPLERDSVLVLVDLGAGRNRMGGSVLAQVYGQYGSE
                     TPDVDAPEQLKAFFDTVQRLNAQRAILAYHDRSDGGLLVTLAEMMFAAHLGVTVDVDG
                     DPLTALFNEELGAVLQVRTENFDAVKTAFEGAGLATHFHRLGMVNTMGALIVRSNGKE
                     IFRKSGMALQR"
BASE COUNT          729 a         1021 c         1096 g          711 t
ORIGIN      
        1 aaaaaagtca ccgtccggtt ggcgtgcatt gcatggccga gaatgcgtcc actatcagcg
       61 tgcaggccac tcggaatatc gattgcaaga atcggaatac cggagccatt gctccagttc
      121 accaagtcag aataatggcc gtccagcgga cgctccagtc cgataccgaa cagcccatca
      181 acaatgagct gccacccagt gctcgctggt atggatacat gtgttgtccc gcctgcggca
      241 tgccattgtt tgaacgcgag atgggcatcg ggcggtaggt tcgcttccga tccggtgaat
      301 aagactgcga cctcgtacca ccattgcttc aggtgaagcg cagcaacaaa cgcatcgccg
      361 ccgttgttgc cgggtccagc tagaaccagc acgcgtttgc cgcgatcacc gagcatgtcg
      421 cgcgcaactt ccgctaccgc cagccccgcc cttgccataa gcggcggtgc ggccggctgg
      481 gacagcagcg agtgttcgat cgtgcggatc tgccgtgcaa gaaagactgg cgcaaatgca
      541 tggggcatag gacagtttaa accacggtga cgctataatt ccgctttcgg gactcaccaa
      601 tgcctaaatg ccagactttc tgacgctgcg cgggcgcaac gccctgtcgg aattccgcct
      661 caacaagctg acccaagcac tcaagaaatc agtcccggca attagcggga tagcaaccga
      721 atactggcat ttcgttcaac tcaaggcacc gcttcagccg ccgcaacgcc ggattctgga
      781 tcggctgctg acctatggtc cggcgccggc gcaagtctcg gagagcggtc gtctgttgct
      841 tgttgtgccg cggctcggga cgatttctcc gtgggcgtcc aaggcaaccg acatcgcaca
      901 ctcctgtggc cttgatgtcg tggaacgcat cgagcgcggc ctcgcctatt acttcacgac
      961 cagcctcggc aaatcgctca acagggcgca gcagcgcgcc gtcctgccgc tgatacatga
     1021 ccgcatgacg caatccgtgc tcgattcact ggatgaggcg aagcagttgt tccagcattt
     1081 tcccgcaacg ccgctcacga cgatcgatgt tacacgcggt ggccgcaagg cgctggagcg
     1141 cgcgaacgtc gagatggggc tggcgctgtc ggatgaagag atcgagtacc tgatggagta
     1201 tttccggcgt gtcgcgcgtg atccgaccga cgttgagcta atgatgttcg cacaagccaa
     1261 ctccgagcat tgccggcaca agattttcaa tgcccagtgg atcatcgacg gcagcacaaa
     1321 ggacaaatcc ctgttcagca tgatccgcca cacccatgcg gctcatccgg cgggaaccgt
     1381 ggtcgcgtat gcggataatg cttccgtcat cgaaggcgca gagatcgaac gcttctatcc
     1441 ggatgcctcg gggagttatc gatattccac ggaacagacc aacatcctga tgaaagtgga
     1501 aacgcataat catccgaccg caatagctcc gttcgaaggg gcggcgaccg gctccggtgg
     1561 cgaaattcgc gacgaaggcg ccaccggccg gggttccaag ccgaaagccg gcctcaccgg
     1621 ttattccgtt tcccatctgc gtatcgcggg ttttgagcaa ccgtgggaaa agcgcaatat
     1681 cggcaagccc gagcgcattg cctcggcgtt gcaaatcatg ctggaagccc cgatcggagc
     1741 agcttccttc aacaatgaat ttggccggcc gaatctgtgc ggctatttcc gcactttcga
     1801 gcacgcggtc gcaagtgaag tgcgtggcta tcacaaaccg atcatgcttg ctggcggcgt
     1861 gggatccatt cctgcgctgc aatcgttcaa gtcaggcgtt ccggctgact ccctcctggt
     1921 gcagatcgga gggccgggca tgctgatcgg catgggagga ggtgcggcgt cgagcatgaa
     1981 caccggtgcg aatatcgccg acctggattt tgattccgtg cagcgtggca atgctgaact
     2041 tcagcgccgg gcgcaagagg ttatcgatcg ctgctgggcc ttgggcacgg acaatccagt
     2101 cttgtcgatt catgatgtgg gcgcgggcgg attgtcgaac gcgctgccgg aactcgttta
     2161 tggggcggga cggggcgggg atatcgacct gcgcgacatt ccttcggaag agccgggcat
     2221 gacgcccgcg cagatctggt gcaatgaagc gcaggaacgc tatgtcctcg cgatcgacga
     2281 ggagcgcctc gccgacttca aagcgttgtg cgagcgtgaa cgttgtccat tcgcgatcgt
     2341 cggcagagcg accgctgacg gcattctgaa agtacgggat ccgcagttca acaacgatcc
     2401 ggtcgacatg ccgctcgaag tggtactcgg caaaccgccg aagatggtcc gcgacgtgcg
     2461 gcggcgcata cgtgttctgc cgcctgccga tttctcaatg gtcgatgttg ccgatgcggt
     2521 gtcacgcgtc ctgcgttttc cagccgtggc caacaagaca tttctgattt cgatcggcga
     2581 ccgcactgtg ggcggactct gctcacgcga tccattcgtc ggaccgtggc aagtgccggt
     2641 cgcggacgtc gccgtgacgc tgatgggatt tgcgacctat cgtggcgaag cgttcgcgat
     2701 cggtgaacgc acccccattg cagttctaaa tgggccagcc tcggggcgca tggctgtcgg
     2761 cgaggcgctg accaatattg cggccgcccc agtggaaaaa ttaggtgacg tgaaactgtc
     2821 cgcgaactgg atggcagcgg cgggttaccc cggagaagac gccatactgt tcgataccgt
     2881 ggaagcggtg gcaatggaat tgtgcccggc gctcggcatc agcattccgg tcggcaagga
     2941 ttcgatgtca atgcgaactg cgtggcagga cggtcacgag aaaaagcagg tcgttgcacc
     3001 gctgtcattg attgtctcgg cctttgcgcc gtgtgtggat gcacggcata cgctgacgcc
     3061 gcaattgcag ccgctggagc gcgatagcgt gctggttctg gtcgatctcg gcgcgggacg
     3121 aaaccgcatg ggtggatcag tgcttgcaca agtctatggg caatacggca gcgaaacacc
     3181 tgacgtggat gcaccggaac aattgaaggc gttctttgac accgtgcagc ggctgaacgc
     3241 gcagcgtgcc atcctggcct atcacgatcg atcggatggc ggtcttctgg tcacacttgc
     3301 ggaaatgatg tttgccgcgc atctcggcgt caccgtcgat gtggatggcg atccgctgac
     3361 cgccctgttc aatgaagagc tcggcgcagt cctgcaggtc cggacggaaa attttgacgc
     3421 agtgaagacg gcgttcgaag gagcagggtt ggcgacgcac ttccaccgtc tcggtatggt
     3481 caataccatg ggcgcgctga tcgttcggtc gaacggcaaa gagatttttc ggaaatcggg
     3541 catggcgttg cagcggg
//
LOCUS       DATMZQ010000498        10089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3758868, whole genome shotgun sequence.
ACCESSION   DATMZQ010000498 DATMZQ010000000
VERSION     DATMZQ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758868"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VN496_15815"
     CDS             complement(<1..600)
                     /locus_tag="VN496_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXF18476.1"
                     /translation="MTASRFFDNRNVLGAVFMLPAAALLVFFLTYPLGLGVWLGFTDT
                     KIGRPGEWIGIENYEFLLTDGVFRLSVYNTFLYTAVASVVKFALGLWLALLLNKHIPF
                     KSLIRAIVLLPFIVPTVLSAIAFWWIYDPQFSIVSWVLVKAGLMDHYIDFLGDPNLAR
                     MSTIIANIWRGVPFVAICLLAGLQTIPPSMYEAASIDGAT"
     gene            complement(705..2018)
                     /locus_tag="VN496_15820"
     CDS             complement(705..2018)
                     /locus_tag="VN496_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXF18477.1"
                     /translation="MGRITRRRFLQASAAAGAALGAPILVPQAHAQELAFKPEAGAKL
                     RVLRWKRFVQGDEDQWLANTKRFTAKTGVEVVTDAENWEDIRPKAAVAANIGNGPDII
                     LSTNDDSHQYPDKLLDVGDLCEYLGKKYGPWYPSALAYGMSSGRWIALPLGSGGNCMN
                     YRESHLKAAGFKEFPKDTDGFLKLCKNLKNNGTPPGFALGNATGDANGWCHWLLWAFG
                     GRLVDEHNKVAINSKETMRMLEYAKELYPTFIPGTLSWLDPNNNKAFLSGDISLTNNG
                     ISIYFAAKNSQDAKMQAIARDMNHAVFPVGPVGHQTMSALFFTCVIFKYTKYPNAAKE
                     YLRFMMEPEQYEAWQQASIGFVTQPLVFYERNPVWTQDPKHTPFKFTCRNMLPNGYAG
                     TLGSASAGCMADFVVVNMVAEAASGNLSVKDAIDKAEKRAKRYYR"
     gene            complement(2042..3106)
                     /gene="ugpC"
                     /locus_tag="VN496_15825"
     CDS             complement(2042..3106)
                     /gene="ugpC"
                     /locus_tag="VN496_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXF18478.1"
                     /translation="MASVELRQVRKLFGNTEVVHDVDVAISDGEFVVLVGPSGCGKST
                     LLRMIAGLEEISSGEIRIGERVVNNIAPKQRDIAMVFQNYALYPHMTVTDNMAFSMKL
                     AGRNAQEIRERVDKAARILGLTEYLERYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTSIYVTHDQIEAMTMADKIVVMNAGRVEQIGSP
                     LELYDHPANLFVAGFIGSPAMNFLKGKLVSASAGRGVQIAPDCVLPAPVSGGSEGREV
                     VIGVRPEHFDIGETGVRAQVVVVEPTGADTQVYCRVAGQDVTVVSRARHTFRPDDTIG
                     LNPSAGKSYLFDPVSGARIV"
     gene            3341..4081
                     /locus_tag="VN496_15830"
     CDS             3341..4081
                     /locus_tag="VN496_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18479.1"
                     /translation="MAGRLSGKVAFVTAAAQGIGRATALAFAREGARVWATDLNEQKL
                     GELKGTPGIEVRKLDVLKDGDVARVAAEVGVPNVIFNCAGFVHHGTILECADKDWDFS
                     FNLNVRSHYVVIRNFLPKMLQAGGGSIINVASVAGSIKGIANRFVYGASKAAVIGLTK
                     SIAMDYVKQGIRCNALCPGTVDTPSLGDRINAFEDPVQARKDFIARQPMGRLAKAEEL
                     AELSVFLASDESVFMTGQAVIMDGGITI"
     gene            4162..5004
                     /locus_tag="VN496_15835"
     CDS             4162..5004
                     /locus_tag="VN496_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXF18480.1"
                     /translation="MKLVRYGEAGKEKPGILDAQGEIRDLSAIVKDIDSAAVSPDGLA
                     KLKAIKTETLPKVSGKPRLGTPIATVPKLICIGLNYADHAKESNLPIPPEPVVFMKAI
                     SAITGPNDEVRLPKGSKKGDWEVELAFVIGKKAQSVSEADALSHVAGYLICNDVSERE
                     WQLEHGSQWSKGKSFDTFAPLGPWLVTADEIKDPQNLAMWLDVNGQRKQTGNTSTMIF
                     GVKKLVSYLSYMCTLSPGDVISTGTPPGVGMGIKPTPQFLKSGDEMRLGIDGLGEQKQ
                     KVSG"
     gene            5069..5245
                     /locus_tag="VN496_15840"
     CDS             5069..5245
                     /locus_tag="VN496_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18481.1"
                     /translation="MLSRGLKAEKLDKDSHKMNDSSEHALHPLHKLRKLWLFMIALVA
                     AAGGIALFGIALAT"
     gene            5255..5851
                     /locus_tag="VN496_15845"
     CDS             5255..5851
                     /locus_tag="VN496_15845"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXF18482.1"
                     /translation="MKEWIYDWGGANVALFHAINANHAPWLDRFMLALTWAGDHNRFA
                     LYVACTALITWWQFVRRPARPTSGEWILALATFSLAYLADGLLVTALKSYLDFPRPSA
                     LFAPDALVVVGPPEFHRSFPSGHASFAALFAAVLWPCAKSHATRIALVVYVIAVCVSR
                     PYLGFHFPADVLFGSLKSVLLVVTVRMALKKCTPQSHV"
     gene            5873..6346
                     /locus_tag="VN496_15850"
     CDS             5873..6346
                     /locus_tag="VN496_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18483.1"
                     /translation="MMIIQVRFVPLLAFVALFAGPACPAEQLDPQESGITAASRPTYS
                     LDAQPQVNRLDFEFRTGESLAWLNSTGDFHVHGWIQHRGLLCATYSMGLRFGAGSPGC
                     LNVEWLSETVYVTSQYQCNGARVEHDGGETMPEIGAKIAQISCAERVVRCSGSCK"
     gene            6337..7167
                     /locus_tag="VN496_15855"
     CDS             6337..7167
                     /locus_tag="VN496_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18484.1"
                     /translation="MQVTWGGQRTTEARNLSVTRTLLIAVLLGGSALFPSLARAELTL
                     SAGIDYLNWSETTTPEVTESGPVYALGLAYTQDRDGGALFAYRGKVWWGKVDYDGATL
                     FGGTPIKSKTNYVGVSNEAQVRWRKAGKPSGNLDGVLGAGLDLWRRSLSTVQTEDYAV
                     GYLRLGVESGTDDAGGWTASVGFKYPVWTYLNAHFDDIGFDSNPILRPGRDVSPYGSL
                     GYRFAPSLQVVGYYDGFRFGRSNAVQANELATGLGPTTLVQPASTMSVFGIRFEYLLR
                     "
     gene            7207..8199
                     /gene="dusA"
                     /locus_tag="VN496_15860"
     CDS             7207..8199
                     /gene="dusA"
                     /locus_tag="VN496_15860"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HXF18485.1"
                     /translation="MTTFDHRLCTAPMMDWSDRHCRYFFRQLSQRAVLYTEMVTTGAL
                     IYGNVERHLAFDAAEHPVALQLGGSDPESLARCARLGERYGYDEINLNCGCPSERVQH
                     GAFGACLMAEPQLVADCIQAMRNAVSIPVTVKHRLGVDQIESYGFVRDFVAALAQAGC
                     TTFLVHARNAILKGLSPKDNREVPPLKYDYVYRLKREFPKLSFVLNGGLKALGAIETH
                     LAQVDGIMLGRLAYHDPYALAQAGLRIFGDAPPSRARVVSQMYTYAIGHVQRGTPLRA
                     IVRHMLGLYHGQPNAKVWRRMLSDAQLLAANRPDLLLDALRATEESAAALAAVG"
     gene            8293..9987
                     /locus_tag="VN496_15865"
     CDS             8293..9987
                     /locus_tag="VN496_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HXF18486.1"
                     /translation="MNPIRFDTYYRYEELVRFLTDWAGTCPGLVRLEPIGRSYEGREM
                     YVVRVTNYKTGPDTDKPALWVDGNIHATELAGSMACLHLLHRLITGYGKDAEVTHCLD
                     TRAFYVCPRVNPDGAEWALADIPKLIRSSTRPYPYDEEPVAGLKREDVDGDGRILTMR
                     VPDKNGPWKQCEQDSRLLVRRDPVESGGRYYRLLPEGRIEAFDGVNVKLQPRRERLDL
                     NRNFPAFWRGEHEQAGAGPFPTSEPEIAAVTSFIARHPNITGAIAFHTYSGVLLRPYS
                     HLADDQMPPEDLWTFQKIGAKGTEITGYPVASAYHDFRYHPKEVITGDFDSWMYDHRG
                     VFAWTVEIWSPQRQAGITDYKYIDWYREHPLEDDLKLMHWNDTVLKGKGFVSWYEFDH
                     PQLGRVELGGWQSLYTWSNPPPHMLEKEIAPFSRWLTWHALISPKLEILEATATSLGN
                     DAWRVRFALQNTGWLPSYVTKMAKNRKLARGVVCEIELPKEARLETGKQREELGQLEG
                     RAYKSAAANTWAGSSADETDDRVSVEWVVCAPIGTSIKLLARHERAGVATTRVTLE"
BASE COUNT         2138 a         2894 c         2942 g         2115 t
ORIGIN      
        1 cgtggcgcca tcaatgctgg ccgcctcgta catagatggt ggtatggttt gcagtcccgc
       61 caaaaggcag atcgcgacaa acggcacacc gcgccagatg ttcgcgatga tggtcgacat
      121 ccgtgccaga ttggggtctc ccagaaaatc gatgtaatgg tccatcagac cagccttcac
      181 cagtacccag ctgacgatgg aaaattgcgg gtcgtagatc caccagaacg caatcgccga
      241 cagcactgtg ggaacgataa acggcagcag cacgatggca cgtatcagcg atttgaacgg
      301 gatgtgcttg ttcagcagaa gcgcgagcca aagacccagc gcgaacttca cgacgctggc
      361 cactgcggtg tacagaaaag tgttgtacac cgagagccgg aatacgccat cggtcagcag
      421 gaattcgtaa ttctcaatgc caatccattc gccagggcgg ccaatcttgg tatcggtaaa
      481 tcccagccag acgccgaggc ccagcgggta cgtcagaaag aaaacaagca acgccgcggc
      541 tggaagcatg aataccgcac ccaggacatt gcggttatcg aaaaagcggg aagcagtcat
      601 gggcgcgtgg cacgccactg ccgggagtcg ctggctacct gcccgccatc gatattggtc
      661 acttcattcg tcacgtcagg ctcggcatta tgaggaggcg cgcgctatct gtaataccgc
      721 ttggcgcgct tctccgcctt gtcgatggcg tctttgacgg acaagttgcc cgaggcagcc
      781 tccgctacca tgttgaccac gacaaaatcg gccatgcaac cggctgatgc gctcccgaga
      841 gtgcccgcgt agccgttggg cagcatattc ctgcaggtga acttgaaagg agtgtgcttg
      901 ggatcctgcg tccagaccgg gttgcgctcg tagaacacaa gcggctgcgt cacgaagccg
      961 atcgacgctt gctgccaggc ctcgtactgc tccggctcca tcatgaagcg caaatactcc
     1021 ttggctgcgt ttggatactt tgtgtatttg aagatcacgc aggtaaagaa cagcgcgctc
     1081 atggtctggt ggccgaccgg cccgaccgga aataccgcgt ggttcatgtc ccgggcgatc
     1141 gcctgcatct tcgcatcctg cgagttcttc gcggcaaaat agatcgagat gccattgttc
     1201 gtcaggctga tgtcgccgga caggaaagcc ttgttgttgt ttggatcgag ccaggacagc
     1261 gtgcccggaa tgaatgtcgg atacagctct tttgcgtatt ccagcatccg catcgtttcc
     1321 ttgctgttga ttgcgacctt gttgtgttca tccaccaacc tgccgccgaa agcccagagc
     1381 agccaatggc accatccatt ggcgtccccg gtcgcgttac cgagtgcgaa ccccggcggc
     1441 gtgccgttgt tcttcaggtt cttgcatagc ttcaggaacc catcggtgtc cttcggaaat
     1501 tccttgaacc ccgctgcttt cagatggctt tcgcggtagt tcatgcaatt gccccccgac
     1561 ccaagtggta acgcgatcca gcgtccgctg ctcatgccgt aggcaagcgc acttggatac
     1621 catggcccat acttcttgcc gaggtattcg cacagatcgc ccacatcgag caacttgtcc
     1681 ggatattggt gggaatcgtc gttggtcgac aggatgatgt ccgggccgtt gccgatattc
     1741 gccgccaccg cggctttggg gcgaatgtct tcccagttct cggcgtcggt gacgacttca
     1801 acgccggttt tcgccgtgaa tcgtttggtg ttcgccaacc actgatcttc gtcgccttgc
     1861 acgaagcgtt tccagcgcag cacccgtaat ttggctcccg cttcaggctt gaaagccagc
     1921 tcttgcgcat gcgcctgcgg taccaaaatc ggcgcaccca gcgccgcgcc ggcagctgcc
     1981 gatgcttgca aaaagcgacg tcgtgtaatg cgacccacgt gctctccttt ccgaagtggt
     2041 atcagacgat ccgggcgccg gaaaccggat cgaacaaata gcttttgcca gcggaagggt
     2101 tcaagccaat ggtatcgtcc ggccggaaag tatggcgcgc ccgcgatacc acggtgacgt
     2161 cctggccggc gacacggcaa tagacttgcg tgtccgcgcc ggtaggctca acgacaacca
     2221 cctgcgctcg cacgccggtc tcgccgatat caaaatgttc cgggcgtacg ccgatgacga
     2281 cttcacgtcc ttcggacccg ccgcttacag gcgccggcag gacgcagtcc ggcgcaattt
     2341 gcacgccgcg cccagcgctt gcgctgacca gtttgccttt caggaaattc atggccggtg
     2401 agccgatgaa acctgcaacg aacaaattag cgggatggtc atagagttcg agcggtgagc
     2461 cgatctgctc caccctgccg gcattcatca cgacaatctt gtccgccatg gtcatggctt
     2521 cgatctggtc atgcgtaacg tagatcgaag ttgtcttcag gcgctggtgc agttccttga
     2581 tttcggtgcg catctgcacg cgcagtttgg catcgaggtt ggacagcggt tcgtcgaaca
     2641 ggaacacttg agggtcgcgc acgatcgcgc gccccatggc gacgcgctga cgctggccac
     2701 cggagagttg ccggggataa cgctcgagat actcggttag tcccagaatg cgcgcggcct
     2761 tgtccacccg ttcacggatt tcctgtgcgt ttcgtcccgc aagcttcatg gagaacgcca
     2821 tgttgtccgt gacggtcatg tgcgggtaga gcgcatagtt ctggaagacc atcgcaatat
     2881 cacgctgctt tggcgcaata ttgttgacca cgcgctctcc tatgcggatc tcgccggagc
     2941 taatttcctc gaggccagcg atcatgcgca acagcgtgga tttcccgcag ccggatggtc
     3001 ccaccaacac gacaaactcg ccatcggaga tggccacgtc gacatcgtga accacctcgg
     3061 tgttgccgaa taatttgcgg acttgacgga gttcgacgga tgccatggct ctatccgtag
     3121 agtcgccaga ccgcgcgaag tttcgcgctg gtgaactggt gcgcggcaaa cactgtttcg
     3181 gaagccatga tccgatttcc ttaactctgg aattgtcgcg atccagtcta gagtaaaacg
     3241 caacgcagca acaaggcggg gcacgttcgc gcgcgcctgt tgggtgctgt ggcatactta
     3301 cgacccttta cgggttggac cataatataa agggggggcc gtggcgggaa gattgagcgg
     3361 caaggttgca tttgtgaccg cagcagcgca gggcatcggc cgcgcaaccg cgctcgcttt
     3421 tgcgcgtgaa ggcgcccgcg tttgggcaac ggatctgaat gagcagaagc tcggagaact
     3481 caaaggcacc ccggggattg aagttcgcaa gctggacgtt ttgaaggatg gcgatgtcgc
     3541 gagagtcgct gccgaagtag gcgtgccaaa tgtaattttc aactgcgccg gattcgtgca
     3601 tcacgggacc atactggagt gtgccgacaa ggattgggat ttttccttca atctcaatgt
     3661 gcgcagtcac tatgtcgtaa tccgcaattt cctgccgaaa atgctgcagg cgggcggggg
     3721 ctccatcatt aacgttgctt cggttgcggg ctcaatcaaa ggcatcgcga accgtttcgt
     3781 atatggcgca tcgaaagctg ccgtaattgg cctgacaaaa tcgatcgcaa tggactacgt
     3841 caagcagggc atccgctgca atgcgttgtg ccccggcacg gtcgatacgc cgtccctggg
     3901 ggaccgtatc aatgcatttg aggatccggt gcaagctcgc aaggacttta tcgcacgcca
     3961 gccaatgggg cgccttgcga aagccgaaga actggctgag ctttcggtat ttcttgcctc
     4021 ggacgagtcg gtgttcatga cgggccaggc cgtaatcatg gatgggggca ttaccatcta
     4081 agcatggtgg cttgattgca agccatgcgt cctgcgtcat aatcacgacg ttcgttgggg
     4141 tcaatagaaa agggaaaaca aatgaaactg gtgcgctatg gagaagcagg aaaagaaaaa
     4201 ccggggattc ttgacgcaca gggcgagatc cgggatttgt ccgccatcgt caaggacatc
     4261 gattcggcag cggtatcgcc ggacgggctt gccaagctaa aagccatcaa gacggagaca
     4321 ctgccaaaag tcagcggcaa gccgcgcctc ggcacaccaa tcgcaactgt gcccaagttg
     4381 atttgcatcg ggttgaacta cgccgaccat gccaaggaat cgaatcttcc gatcccaccc
     4441 gagccggtcg tgttcatgaa agcgatttct gcgattaccg gaccgaatga cgaagtaagg
     4501 ctgcccaaag gttcaaaaaa gggcgattgg gaggtcgaac ttgcattcgt cattggcaaa
     4561 aaagcgcaaa gcgtttctga agcagatgcg ctgagtcatg tcgccggtta cttgatctgc
     4621 aatgacgtct ccgaacgtga atggcaactg gagcatggca gccagtggtc caagggcaag
     4681 agctttgata cctttgcgcc tcttgggccg tggctcgtga cggcggacga aatcaaggac
     4741 ccgcaaaacc tcgccatgtg gctggacgtc aatggtcagc gcaagcagac cggcaacacc
     4801 agcacgatga tattcggggt gaagaagctg gtttcgtatt tgtcatacat gtgcacgctt
     4861 tcaccagggg atgtgatcag caccggcaca ccgcccggtg tcggcatggg gatcaaaccc
     4921 acgccgcagt tcctcaagtc cggcgacgaa atgcgccttg gcatcgatgg cctcggcgaa
     4981 caaaagcaaa aagtgtccgg ctgatcacgg gcagaaaccg aaccgactca aagcgatttt
     5041 cacagaggcg caccgcggtg cgcctctgtt gctttcgcgc ggactcaagg cagaaaaact
     5101 ggacaaagat tcgcacaaga tgaatgacag cagcgaacat gccctgcatc ccctccataa
     5161 actgcgcaaa ctctggctat tcatgattgc gctggtagcc gctgccggtg gcatcgcatt
     5221 atttggcatc gcactggcga cctaggaatt cgccatgaaa gaatggatct acgactgggg
     5281 cggggccaat gtcgccctgt ttcatgcgat caatgccaac catgcgccgt ggctcgaccg
     5341 gttcatgctg gcgctgacct gggctggcga ccacaatcgc ttcgcccttt atgtcgcttg
     5401 caccgcactc attacctggt ggcaattcgt gcgacgcccc gcgcgcccga cttcaggtga
     5461 atggattctg gcgttggcga cattttcact cgcttacctg gcggatggtt tgctggtgac
     5521 cgcgctgaag tcctatctgg attttccgcg tccctccgcc ctgtttgcac cggacgctct
     5581 tgttgtcgtg ggtccgcccg aattccaccg cagctttccg agtggacacg catcgttcgc
     5641 ggcgctcttc gccgccgtgc tatggccatg cgccaagagc catgcgacgc gcatcgcatt
     5701 ggtcgtttac gtgattgcag tatgcgtctc gcgcccttat ctcggctttc actttccggc
     5761 agacgtactg ttcggcagct tgaaatctgt gctgctcgtt gtcaccgtcc ggatggcgct
     5821 gaaaaaatgc acgccgcaat cgcacgtctg acaggttacg ttatgatgtc gcatgatgat
     5881 cattcaggtc cgcttcgttc cactgctggc ttttgtcgcg ctgtttgccg gccccgcgtg
     5941 tccggcagag cagctggacc cacaagaatc cggcatcacc gccgcgtcgc gcccgaccta
     6001 ttcactggac gcgcagccgc aagtcaaccg cctggatttc gaattccgca ccggcgaatc
     6061 attagcatgg ctcaacagca ccggcgattt tcatgtgcac ggctggattc agcatcgggg
     6121 acttctgtgc gcgacctata gcatgggcct gcgattcgga gccggttcgc cgggctgcct
     6181 gaatgtcgaa tggctgtccg aaacggtata tgtcacctcg caataccagt gcaacggtgc
     6241 cagggtagag catgacggcg gtgaaacgat gcctgaaatc ggggccaaga ttgcacaaat
     6301 ttcctgtgct gaaagagtgg ttcgctgcag cggatcatgc aagtgacatg ggggggtcag
     6361 cggaccaccg aagcccgtaa cctttccgtc actcgcacac ttctgatcgc cgttttgttg
     6421 ggcggcagtg ccttattccc ttcactggcc cgcgcggaac ttactctgag cgccggcatt
     6481 gattatctga actggagtga aaccacaacg ccggaagtca ccgaaagcgg tcctgtgtat
     6541 gccctcggcc tggcctacac gcaagaccgc gacggcggtg cgcttttcgc ttaccgggga
     6601 aaggtatggt ggggcaaggt cgattacgac ggcgccaccc tgtttggtgg cacgccgata
     6661 aaaagcaaaa caaactacgt cggtgtcagt aacgaggcgc aggttcgctg gcgcaaggcc
     6721 ggcaaaccat ccggcaacct tgatggcgtt ctaggcgcag gactggacct gtggcgccgg
     6781 tcgctatcga ccgttcaaac agaagattat gccgtcggct atttgcgact gggtgtggaa
     6841 agcggcactg acgatgccgg cggatggacg gcatccgtgg gattcaaata ccccgtatgg
     6901 acttacctga acgcgcattt cgacgatatc ggattcgatt ccaatccgat tctgcgtcca
     6961 ggcagagacg tgtctcccta cggcagcctg ggctatcgtt tcgcgccgtc gcttcaagtc
     7021 gtcggctact acgatggctt ccgtttcggc aggtcaaatg cggtacaggc caatgaactc
     7081 gcgaccggct tggggccaac cacgctggta cagccggcga gcaccatgtc ggtctttggc
     7141 attcggttcg aatacctgtt gcgctaacat atcccccgtt tgttgccgcc tcagtgagcc
     7201 attggcatga cgacgttcga tcaccgcctg tgcactgcgc cgatgatgga ctggtcagac
     7261 cggcactgcc gttatttttt caggcagcta tcacagcgtg ccgtgctcta caccgagatg
     7321 gtcaccaccg gcgcactgat ttatggcaac gtagagcggc atcttgcgtt cgatgcggcg
     7381 gaacatccag tcgcgctgca actcggcggt tccgatccag aatcgctggc tcggtgtgcc
     7441 cgcctggggg agcgatacgg gtacgatgaa ataaacctga actgcggttg tccctcggag
     7501 cgcgtacagc atggcgcatt cggcgcctgc ctgatggcgg agcctcagct cgtcgcagat
     7561 tgcatccagg cgatgcgcaa tgcggtttcg atcccggtga ccgtcaagca ccgtcttggc
     7621 gtcgatcaga tagaaagtta tggattcgtc cgggattttg tggcggcact ggcacaagcg
     7681 ggctgcacga cgtttttggt gcacgctcgc aatgcaatcc tgaaaggact ttcccccaaa
     7741 gacaatcgcg aggtaccacc gctgaaatac gactatgtct accgattaaa acgggaattt
     7801 cccaaattga gctttgtgct caatggcggc cttaaggcgc tgggcgcaat cgagacacac
     7861 ctggcgcagg tcgatggcat catgctggga cgcctggcgt atcacgatcc gtatgcactt
     7921 gcgcaggccg ggctgagaat tttcggcgat gcaccaccat cgcgtgcgcg cgtagtgagc
     7981 caaatgtata cctatgcgat cggtcacgtg caacgcggca ccccgctgcg cgcaattgtc
     8041 cgtcacatgc tgggcctcta tcatgggcag ccaaatgcga aagtgtggcg gcgcatgctc
     8101 tcggatgcac aattgcttgc cgctaatcga cccgatcttc tgctcgatgc cctgcgcgcc
     8161 actgaagaaa gcgcggcggc gttggccgca gtgggctgac agcgccggtc atgcagaggc
     8221 ggcgagcacg actggcatga cattgtcgct tgcacattct gctatcgttt atcagcaaag
     8281 cctttcccaa gcatgaaccc aattcgcttc gacacgtact atcgctacga agagctggtg
     8341 cgcttcctga ctgactgggc aggcacctgt ccggggctgg tgcgcctgga accaatcggc
     8401 cgaagttacg agggccgtga gatgtatgtc gtacgcgtga cgaattacaa gacggggccc
     8461 gacactgata aacccgcgct ttgggtcgat ggcaatattc acgcaacgga gctggccggc
     8521 tcgatggcgt gtctgcactt gctgcaccgg ttgattaccg gctacggcaa ggacgcggag
     8581 gtcacccatt gcctcgacac acgcgcgttc tacgtctgtc cacgcgttaa tccagacggg
     8641 gcggaatggg cactggcaga cattccgaaa ttgattcgct cgagcacgcg gccatatccg
     8701 tatgacgagg aaccggtcgc gggactgaag cgagaagatg tcgacggtga cggcaggatt
     8761 ttaaccatgc gggtgccgga taagaatggt ccctggaaac agtgcgagca ggattctcgc
     8821 ctgctggtgc gtcgcgatcc cgttgagagc ggcgggcggt actaccggct gctgccggaa
     8881 ggccgcattg aagcttttga cggtgtgaac gtaaaactgc aaccgcgccg cgagcgattg
     8941 gacctaaacc ggaattttcc ggcgttctgg cgcggggaac atgagcaggc aggtgccggc
     9001 ccgtttccga cgtctgagcc ggaaattgct gcagtcacca gcttcatcgc acgccatccc
     9061 aatatcaccg gtgcaattgc gtttcacacc tacagcggcg tgctgctgcg cccctattcg
     9121 catctggccg atgatcagat gccacccgaa gacttgtgga ccttccagaa aatcggcgcg
     9181 aagggcacag aaataacagg ctacccggtg gcctctgcgt atcacgattt tcgctaccat
     9241 ccgaaggaag tcattacggg cgattttgat tcgtggatgt acgaccaccg cggtgtgttt
     9301 gcctggacgg tggaaatctg gagtccgcaa cggcaggccg ggatcaccga ttacaaatac
     9361 atcgactggt atcgggaaca cccactcgag gacgatctga agctcatgca ctggaacgac
     9421 accgtgctca agggcaaggg cttcgtttca tggtacgagt tcgaccaccc tcaacttgga
     9481 cgcgtggaac tcggtggctg gcaaagtctt tacacctgga gcaacccgcc accgcacatg
     9541 ctcgaaaaag aaatcgcacc gttctcacgc tggttgacgt ggcatgcact gatttcgccg
     9601 aaactggaaa tacttgaagc aacggccacg tcacttggca acgacgcctg gcgtgtgcgc
     9661 ttcgcgctgc agaataccgg ttggctaccg agctatgtga cgaaaatggc caagaacagg
     9721 aagctcgcgc gcggtgttgt atgtgaaatc gaactgccga aagaggcgcg actcgagacc
     9781 ggcaagcaga gagaagaatt aggccagctt gagggccgtg cgtacaaatc ggccgctgcc
     9841 aatacctggg ccggatcgag cgcagatgaa acggacgacc gcgtgagcgt cgaatgggtc
     9901 gtgtgcgcgc ccatcggcac cagcatcaaa ctgctcgcgc gtcatgagcg ggctggcgtc
     9961 gcaaccactc gcgtaacgct cgaatgaatg acctgtcatg agaacttccc gggcaaccat
    10021 caataccatc ggcgcagctt tcttcgccac ggcgcttccc cctcttgcca ttgccctcgg
    10081 ctggctcat
//
LOCUS       DATMZQ010000499         7759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4449940, whole genome shotgun sequence.
ACCESSION   DATMZQ010000499 DATMZQ010000000
VERSION     DATMZQ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4449940"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VN496_15870"
     CDS             complement(<1..1054)
                     /locus_tag="VN496_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXF18487.1"
                     /translation="MKQNFDLLIRNASIIDGSGAARFEGDIGVRADRVVKIGRLDAAR
                     GDIELDASGKVAAPGFIDAHTHDDRLMLSAGDMAPKVSQGVATVVAGNCGISLSPMPD
                     GMKGAAPAPLDLLDNEGTWFRFRTFGAYVEELAAHPAATNCALLVGHMTLRVVTMDRL
                     DRPATTAEIGRMRGLVREALGSGAIGFSSGLYYELSNAAPAEEVVQIAQPLKEFNGIY
                     CTHMRDEAEQVLESLDESFRAGRELGVPVVISHHKVVGSKNHGRSRETLPNIAEAMRV
                     QPVCLDCYPYMASSTMLSYARTLICSRVIVSSSKPYPQFAGMDLDEVVKIMGLPVEEA
                     IAKLQPASAIYFSMDET"
     gene            complement(1234..2190)
                     /locus_tag="VN496_15875"
     CDS             complement(1234..2190)
                     /locus_tag="VN496_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXF18488.1"
                     /translation="MKRGCFAAMLLVLLCGPALADYPDKPIRFIVPSAAGGSPDTLMR
                     ILTAELSRRLGVQIVILNKPGANAVIGTMEIVHAAPDGYTLGYGNIVSLAIVHGLVAS
                     VPYRNEDLTLISNCLRVANLLAVNKDLPVHSVRELIDYGHKYPDKLIMASAGNGTTGH
                     LGGELFKAMTGTPMLHIPYRGSPQAIADVIAGEANVIFDNLTSLSSHARSGRVRPLAV
                     SGAHRSPLFPGLPTIAEAGVAGYETVAWGGIIGPAKLPPEIVAKLNREIIAALHSPAV
                     SKRFAELDTEVDGSSPQQFAAIASRERPRWAEIIKRAGAKVE"
     gene            complement(2244..3836)
                     /locus_tag="VN496_15880"
     CDS             complement(2244..3836)
                     /locus_tag="VN496_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXF18489.1"
                     /translation="MSKLVASNALALILFAGQLFAAPGPTQQELNDASASTEWPLPNH
                     DYAGVRYVKLGQIRPDNAASLRPVCSFQGADLNRALNNPIVYGGVMYVTTLYSTIALD
                     PASCKVKWRHEWKPKGREANGSIKNRGVAIKDGKLVRGAQDGWMFALDAQTGKLLWEV
                     KAANAEKYEALSINPLLFEDLVIIGPSGSEFGVKGWIGAFKLEDGAAVWRFNTIPDDG
                     EAGAETWGDADSRLKGGGGIWTTPALDVNSGRLYVAVGNPAPDMFGSLRPGTNLYTNS
                     IVVLDVRTGALLWHKQAVPHDTHDWDMPVTAPLFKATIGGKQRDVVTMAAKDGVMRLV
                     DRNTREELYAVEVATRTNADREPTEDGVYTCPGLAGGVEWSVPAYSPKLDLMVVPAVD
                     WCGVFKKEDEARFVAGQQFLGGSFTWDPVEKSAGWLTAVKATTGEQLWRYRANRPMLA
                     AVTATSGDMLFTGELSGDFLALDARTGKVVYRYSGGGAIVGGVITYAVAGKQYVAAVS
                     GMAAGFWLGPPGSMTVTVFALP"
     gene            complement(3877..4287)
                     /locus_tag="VN496_15885"
     CDS             complement(3877..4287)
                     /locus_tag="VN496_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF18490.1"
                     /translation="MTAPFCNRVFLALCFVLGASSAAAEEGVYSSAQAARGEETYQQF
                     CSACHGARLQGAPAAPLTGESFRARWEDGKHTLDDLYYIIRSLMPNNSPGSLSKAQYA
                     DVVAFILKINNYPAGEQELVPKASVMKAVTLQPH"
     gene            complement(4308..4781)
                     /locus_tag="VN496_15890"
     CDS             complement(4308..4781)
                     /locus_tag="VN496_15890"
                     /inference="COORDINATES: protein motif:HMM:NF044909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18491.1"
                     /translation="MTLIRTAAISLVCILALRVPASAQDAFEDVRQTLQAYEQAWSRH
                     DASAIAAFYFEPAMRVSKAGPVIRATRADQEAFFAGYLRAIIERGYERSAWESLNGRL
                     LDAQTALASGIAVRYRADGSILERVGLTYHLWKTADGWKIFLSATHTPENVLRFQ"
     gene            4925..5779
                     /locus_tag="VN496_15895"
     CDS             4925..5779
                     /locus_tag="VN496_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HXF18492.1"
                     /translation="MASTAAVSPVWFITGCSTGLGRALAQAVLHEGHRAAVTAREPAR
                     VRDIVASYPATSLALQLDVANYTQMEQAVAAAEKKFGRIDVLVNNAGYGYLAAVEEGE
                     EAEIRAMFETNFFGLAALIRRVLPGMRARAQGHIINISSIGGLLGNPSAGYYNATKFA
                     VEGLSEALAKEVETLGLRVTVVEPGPFRTDWAGRSLKKPRVAIAAYEGTAGARRAQIT
                     GYSGKQPGDPSRAAQAIIRVVESPAPPMNLVLGKEGLKRVREKLDRFSSTLRTWEATT
                     TAADFPDA"
     gene            5780..6772
                     /locus_tag="VN496_15900"
     CDS             5780..6772
                     /locus_tag="VN496_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HXF18493.1"
                     /translation="MMPTSIRVAVLDDWQGMARQSADWSTLQQRAEIVFFEAPFQDAD
                     ALVNALAGCDIIIAMRERTKFTRALLERLPKLRMIALTGGRTWTMDFDTLNARGIPVC
                     HTGGEKSGAATAEIALGLLLSAARFMNRGDASIRSGGFQGGVPAGDVLDGRTLGIIGL
                     GKIGARMARYGLALGMRVIAWSVNLTAEKAGAAGALLVDKSTLLNQSDAISLHLVLSD
                     RTRGTLGAKELAQMKSGAILINTSRGPLVDEPALLQSLKSGRLIAGLDVFEQEPLPPD
                     HPLRSLPNTVLTPHLGYCARDVYAQFYRESVENVLSFLDGNPQRVLNPEALRSA"
     gene            6994..>7759
                     /locus_tag="VN496_15905"
     CDS             6994..>7759
                     /locus_tag="VN496_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXF18494.1"
                     /translation="MPAIAAMSTRNAWPALWAACISISAAAAGGDWPMVAKDYANTRY
                     SELGEISVANAANLKLAFTFSTGVLRGHEAAPLVVDSVMYIVTPYPNYLYALDLSKPG
                     APPKWKYEPKPLAFAQGMACCDVVNRGVAYANGRIFMNTLDNHTVAVDAKTGKELWRT
                     RLGDVALGESMTMAPLVVKDKVLVGNSGGEFGVRGWLAALDATSGKIVWRAYSTGPDN
                     EALIGQEFRPFYSQDQGKDLGVSSWPPEAWRIGGGAV"
BASE COUNT         1579 a         2354 c         2257 g         1569 t
ORIGIN      
        1 cggtctcgtc catcgagaag taaatagcag aagcaggttg cagcttggcg atggcttcct
       61 ccaccggcaa gcccatgatc ttcacgactt catccagatc catgcccgca aactgcggat
      121 acggcttgga cgaactgacg attacacggg agcagatcaa ggtgcgcgcg taactgagca
      181 tggtcgaaga ggccatgtat ggataacagt ccaggcagac tggttgcacg cgcatggctt
      241 cggcgatgtt cggcagggtt tcccgcgacc gtccgtggtt cttgctgccc acgaccttat
      301 ggtgcgaaat gactactggc acaccgagtt cgcgacccgc gcgaaaactc tcgtccagcg
      361 attccaggac ttgctctgct tcgtcacgca tatgcgtgca atagatgccg ttgaattctt
      421 tcagcggctg agcaatctgc acgacttctt cagcaggcgc ggcgttggat aattcatagt
      481 acaagccgct cgaaaagccg attgcacccg acccaagggc ttcgcgcacc agaccgcgca
      541 tgcgcccgat ttccgccgtg gtagctgggc gatccaagcg atccatggtg acaacgcgca
      601 acgtcatgtg tccgaccagc aacgcacagt tggtcgccgc cggatgcgcg gccagttctt
      661 ccacataagc accgaaggtc cgaaagcgaa accacgtgcc ctcgttatcg agcaggtcca
      721 gcggcgccgg cgccgcaccc ttcatgccat cgggcatcgg ggacaaggag ataccgcagt
      781 tgccggcaac aacggttgcg acgccctggc tgactttcgg cgccatgtcg cctgccgaga
      841 gcattaaccg atcatcatgc gtatgcgcat cgatgaagcc gggcgccgcg actttgccgg
      901 aagcatccag ctcgatgtcg ccccgggcag catcgagccg gccaattttc acgacgcgat
      961 ctgcgcgcac gccaatgtcg ccctcaaagc gagcggcccc gctgccatca ataatgctcg
     1021 catttctgat cagcagatcg aaattttgtt tcatggttat tccattgtta gaccaggata
     1081 caagacgtac aaaaccggag gctactgagc aagcgagaac ggaatctgat tgactcgatg
     1141 ttgccgcagg ctcgcacgga catcgcggcg ctggtcctct tttccttcac tttgtccccc
     1201 gtgggcccgt gttgtacgcg gttcaaatga gcatcactca actttcgcgc cggcacgctt
     1261 gatgatttct gcccaacgcg gccgttcgcg actggctatc gcggcaaact gctgcggact
     1321 gctgccgtcc acttcggtat cgagctcggc gaaacgcttg cttacggccg gggaatgcaa
     1381 tgcagcgatt atttcgcggt tcagctttgc cacgatttcc ggcggcaact tcgccgggcc
     1441 gatgataccg ccccatgcga ccgtctcgta acccgccacg ccggcttcag cgatggtcgg
     1501 caggccggga aacaaaggcg accgatgcgc gccggatacc gcaagcggcc gcacgcgtcc
     1561 agacctcgcg tgcgaggaca atgaagtcag attgtcgaag atgacgttgg cttcgcctgc
     1621 aatcacatcg gcgatcgcct gcgggctgcc gcgataagga atatgcaaca ttggcgtgcc
     1681 cgtcattgcc ttgaataatt caccgccaag atgaccggtg gtaccgttgc cggcagaagc
     1741 catgatcaac ttgtccggat acttgtggcc gtaatcgatc aattcccgga cgctatggac
     1801 cggaagatcc ttgttgaccg caagcaggtt cgcgacgcgc aggcaattgg agatcagcgt
     1861 cagatcctcg ttgcgatacg gtacagacgc gacgagcccg tgcacgatgg caagggaaac
     1921 gatgttgccg taccccaacg tataaccgtc cggcgctgca tggacgattt ccatcgtgcc
     1981 gattaccgcg ttggcgccgg gtttgttcaa gattacgatt tgcacgccga ggcgcctgga
     2041 taattccgca gtcagaatgc gcatgagtgt atcgggcgac ccgccggcgg cagagggcac
     2101 gatgaaacgg attggcttgt ctggataatc ggcaagcgcc ggcccgcaaa gcaggaccag
     2161 cagcattgcg gcgaagcagc ctcgcttcac ttgtttcttc ctgtacggct actactcagt
     2221 ctcactgttt tctatcgtcg atttcaagga agcgcgaaca ccgtcaccgt catcgatccc
     2281 ggcggcccga gccagaatcc agcagccatc ccggagacag cagcgacata ttgtttgccc
     2341 gccaccgcat aagtaatcac accgcccacg attgcgccgc caccgctata tcgatagacg
     2401 accttgcccg tacgtgcgtc cagtgccagg aaatcgccac tcagttcgcc agtgaacagc
     2461 atgtcgcccg aggtcgcagt cacggcggcc agcattgggc gatttgcgcg gtaacgccag
     2521 agttgctccc cggtcgtcgc ctttacggct gtcagccagc ctgcggattt ttccaccggg
     2581 tcccaagtaa aagagccgcc caggaactgc tgtcctgcca cgaagcgggc ttcgtcctcc
     2641 tttttgaaaa cgccgcacca gtcaaccgct ggcaccacca tcaggtcaag cttcggactg
     2701 taggccggga cgctccattc cacgccgccc gccaggcccg ggcaggtata gacgccatcc
     2761 tccgtcggtt cgcgatcggc gttggttcgc gtagcgactt ctacggcgta aagttcttcg
     2821 cgggtattgc ggtccaccag acgcatcaca ccgtctttcg ccgccatggt gacgacatcg
     2881 cgctgcttgc cgccaatcgt ggctttgaac aagggcgccg tgaccggcat atcccaatca
     2941 tgcgtgtcgt ggggcaccgc ctgcttgtgc cacagcagcg ctccagtgcg cacatccagc
     3001 accacgatgg aattggtgta gagattggta cccggccgca gactcccaaa catatcgggc
     3061 gctggattgc cgaccgccac gtaaaggcgt ccgctgttca cgtcgagcgc cggcgtagtc
     3121 cagatgccac cgccaccctt tagccgcgaa tctgcgtcgc cccaggtttc cgcgccggct
     3181 tcaccatcgt ccggaatcgt attgaatctc cagaccgcag caccgtcttc cagcttgaat
     3241 gcgccgatcc atccctttac gccgaactcg gagccggaag ggccgatgat gaccaggtcc
     3301 tcaaacaaca gcggattgat cgaaagcgct tcgtattttt cggcattggc cgctttgacc
     3361 tcccagagca gcttccccgt ttgtgcgtcg agcgcaaaca tccaaccatc ctgtgcaccg
     3421 cgtactagct tgccatcctt gatggccaca ccgcgatttt tgatagagcc gttcgcttcc
     3481 ctgcccttcg gcttccactc atgtcgccat ttcaccttgc aagatgcagg gtcgagcgca
     3541 atggtcgaat aaagcgtcgt tacatacatc acgccgccat atacgatcgg gttattcagc
     3601 gcgcgattca gatccgctcc ctggaagctg cagacgggac gaagcgaagc ggcattgtcg
     3661 ggcctgatct gaccgagctt tacatagcgc accccggcgt agtcatgatt gggtagcggc
     3721 cactcggtgc tggcagacgc atcgttgagt tcttgttgtg tggggccggg cgcggcaaac
     3781 agctggcccg caaatagaat cagtgccaaa gcgtttgatg cgaccagctt gctcatgatt
     3841 gctgccccct tgtgtctgta tgtctttcaa tcgatttcaa tgcggctgca gtgtgactgc
     3901 tttcatcact gaggctttgg gcaccagttc ctgctccccg gccgggtagt tgttgatctt
     3961 caaaatgaag gccaccacat cggcatactg cgctttgcta agcgagccag gcgagttatt
     4021 gggcatcagg ctgcgaataa tgtagtacag atcatcgagc gtgtgcttgc catcttccca
     4081 gcgtgcgcga aatgactccc ccgtgagcgg tgcggcgggc gcaccctgca gccgcgcgcc
     4141 atggcatgcc gagcaaaatt gctgataggt ttcttcgccg cgtgctgcct gtgccgatga
     4201 atagacccct tcctcggctg cagcgcttga agcgccaagc acaaagcata aggccaggaa
     4261 aacccgattg caaaatggtg cggtcacaga atgtcgtccc gagcgcctta ctggaatcgc
     4321 aggacgtttt ctggcgtatg ggtcgcagac aagaatattt tccagccgtc ggcggttttc
     4381 caaagatgat aagttaggcc cacccgctcc agtatcgatc catccgcgcg atagcgcacc
     4441 gcaatgccgc tggccagggc tgtctgcgca tcgagcaggc ggccgttcag tgattcccat
     4501 gcgctacgct cgtaaccccg ttcaatgatg gcgcgcaaat agcccgcgaa aaaagcttcc
     4561 tgatccgcac gagtcgcacg aatgaccgga cccgccttgc tgactcgcat cgccggttca
     4621 aagtagaagg ctgcgatggc cgaggcgtca tgcctgctcc acgcctgttc gtacgcctgc
     4681 aatgtctggc gaacgtcttc gaaagcgtct tgcgcggagg ctggaaccct gagcgcaaga
     4741 atgcagacga gggagatcgc agcggtgcgg attaatgtca ttttgtcgct cctgtcgagg
     4801 agagtagtcg gccgatttgc atgcaagctt gccaagattc ttgccacgcg ccatccttgc
     4861 ggattatgcg gctcatggca aattgacgtt aatctgcgcg cacactaata cagaaggaac
     4921 atccatggcc tccactgccg ctgtttctcc ggtctggttc atcaccggtt gctcgacagg
     4981 tctcggccgc gcactggcgc aggcggtact gcacgagggc caccgggcag ctgtgaccgc
     5041 gcgcgaacca gcccgagtcc gggatatcgt tgcaagctat cccgccactt ccctggcatt
     5101 gcagctcgac gttgcaaatt acacccagat ggaacaggcg gttgccgccg cggaaaagaa
     5161 attcggccgc attgacgtgt tggtcaacaa cgcaggctat ggctatctcg cggctgtcga
     5221 agaaggcgag gaggcggaaa tccgcgcgat gttcgaaaca aacttttttg gcctggctgc
     5281 tttgatccgg cgcgtgcttc ccggcatgcg cgcccgcgcc caaggacaca ttatcaatat
     5341 ttcttcgatc ggcggactgc ttggcaatcc gagcgctgga tactacaacg ccacgaaatt
     5401 cgcagtggaa ggtttgtcgg aagcgctggc gaaggaagtc gagactctcg ggttgcgcgt
     5461 taccgtcgtc gagcctgggc cgttccgcac cgactgggct ggacgttcgc tgaaaaaacc
     5521 gcgcgtagcc atcgcagcgt acgaagggac ggccggcgca cgccgtgcac agattaccgg
     5581 gtatagcggc aagcaaccag gcgatccctc gcgggccgcg caggccatca tccgggttgt
     5641 agagtcacct gctccaccga tgaatctggt actcggtaag gaaggactca agcgcgtgcg
     5701 cgagaaactg gatagattct ccagcacctt gcgaacgtgg gaagcgacca cgacggctgc
     5761 cgactttccg gatgcctaga tgatgccgac ttccatacgc gtcgccgtac tggacgactg
     5821 gcaaggcatg gccaggcaaa gtgccgactg gtcgaccctg cagcagcgcg ccgagatcgt
     5881 attctttgag gcgccgttcc aggacgcgga cgcactggtg aacgcactag ccggttgcga
     5941 catcatcatc gccatgcgcg agcgcaccaa attcaccagg gcgctgctgg aacggctgcc
     6001 gaaattacgc atgattgcac tgacgggcgg gcgcacctgg accatggatt tcgatacgct
     6061 gaacgcgcgc ggcattccgg tatgtcatac cggcggcgaa aaatccggtg cagcgaccgc
     6121 ggaaatcgca cttggcctgt tgctgtctgc ggcaaggttc atgaaccgcg gcgatgcgtc
     6181 gatacgcagc ggcggctttc agggcggcgt gcctgcggga gacgtgctcg acgggcgcac
     6241 tctcggcatc atcggcctgg gcaaaatcgg cgcgaggatg gcgcgttatg gactggcgct
     6301 cggcatgcgt gtcatcgcgt ggagcgtgaa ccttaccgca gaaaaggccg gcgctgccgg
     6361 cgcgctgctc gtcgacaaga gcacgctttt gaaccagtcg gatgccattt cgctgcatct
     6421 ggtgctgtca gatcgcacgc ggggcacgct gggtgcgaaa gaactcgcgc aaatgaaatc
     6481 cggcgcaatt ttgatcaata cttcgcgcgg ccccctggtg gacgaaccgg ctttgctgca
     6541 aagcctgaag tcgggccggc tgattgccgg cctggatgtc ttcgaacagg aaccattgcc
     6601 gccggatcat ccgctgcgca gcctgcccaa caccgtgttg acgccccacc tgggttactg
     6661 cgcgcgcgac gtgtatgcac agttctatcg cgagtcggtt gaaaacgtgc tgtcatttct
     6721 ggacggcaac ccacaacggg ttctgaatcc cgaagcgctt cgcagcgcct gatttacctc
     6781 acaccgtgaa tttgtactag agcgcgtctc tggtagtttt tgccggtccg atgcaaaaac
     6841 taccgccgcg atccggtgat tgcgtcgcct gcacgttgta aaagcgcgca actctgttcg
     6901 taacgatctg gcatatgact tgcgcgccgc tgctgatgag attcgtccac gatctgcttc
     6961 gcggacacga ctcgcctcag gagatcgccg ggcatgccag ctattgctgc aatgtcgacc
     7021 cgcaatgcat ggccagctct gtgggcggca tgcatttcga tcagcgctgc ggccgctggc
     7081 ggcgattggc ccatggtcgc aaaggactat gccaatacac gctatagcga gcttggcgag
     7141 atcagcgttg cgaacgcagc taacctgaag ctcgcattta ccttctcaac cggcgtgttg
     7201 cgtggacacg aagcggcccc actggtcgtc gatagtgtga tgtacatcgt cacgccgtat
     7261 ccgaactacc tgtacgcgct cgacctcagc aaaccgggtg caccgccgaa atggaaatac
     7321 gaacctaagc cgctggcctt tgcgcaaggc atggcgtgct gcgacgtcgt caaccgcggc
     7381 gtagcttatg ccaacggccg catttttatg aacacgctcg acaaccatac cgtggcggtc
     7441 gatgccaaga ccggcaagga actctggcgt acgcgtctgg gagatgtcgc gctcggggaa
     7501 tcgatgacca tggcgccgct ggtggtcaag gacaaagtgc tggtcggcaa cagtggcggc
     7561 gaattcggtg tgcgcggctg gctggcggca ttggatgcta catccggaaa gatcgtctgg
     7621 cgggcataca gcacagggcc cgacaacgaa gcactgatcg gtcaggagtt caggcctttc
     7681 tattctcaag accagggaaa agatctcggc gtgtcgagct ggccccctga ggcgtggcgc
     7741 atcggcggag gcgccgttt
//
LOCUS       DATMZQ010000500         3896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3763535, whole genome shotgun sequence.
ACCESSION   DATMZQ010000500 DATMZQ010000000
VERSION     DATMZQ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763535"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..548
                     /locus_tag="VN496_15910"
     CDS             318..548
                     /locus_tag="VN496_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18495.1"
                     /translation="MTKTLSALAAGLFALTLGFGAHAASDQYKVAKKQADTSYKSAKA
                     ECKKMSGDEKKNCMNKAKADHQAALDRAKTMK"
     gene            complement(617..838)
                     /locus_tag="VN496_15915"
     CDS             complement(617..838)
                     /locus_tag="VN496_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18496.1"
                     /translation="MKSSPDIIQGKKRIEVWQQARDLALTGTFENFDKVVQALKTLGL
                     DAGLDPGRVLACERGTINKLCARSRRSNK"
     gene            complement(1062..1265)
                     /locus_tag="VN496_15920"
     CDS             complement(1062..1265)
                     /locus_tag="VN496_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18497.1"
                     /translation="MGIERRKKLYRRERGTDVWHLSPECEGWPVWNFVERLAPGAGVI
                     CPECLASHPEIPRRRKADLKKTR"
     gene            complement(1784..3739)
                     /locus_tag="VN496_15925"
     CDS             complement(1784..3739)
                     /locus_tag="VN496_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF18498.1"
                     /translation="MSAPIRILHLEDDAADARLIREQLLRSGLDVLITVAPGREAFEA
                     EIRKTNFDIILSDYRVPRFNGLQALEYVRSAAIHVPFILVTGALGDEGAIELLHSGAT
                     DCVLKDRLARLAPAIQRALREDAARKKHAQVQAQLLEANRLIKLAAEAARLGTWQLDV
                     ASGKLACSDEFLDLINVRKADWTGTIEALEALMHPEDVVTRRRVHAHSIEHGLFMEFE
                     FRVEMPDGEIRWMHLRGDCTHESATSRSTYLGVMMDITARKKLEGALREADRRKDEFL
                     ATLAHELRNPLAPIYNGLSVLRQSAVESAEQTQIHAMLERQTNHMIRLVDDLMDVSRY
                     TMGKIELKLVPIELQTVLRHAIEMSQPLIGAGRHQLDLSVPTEPVMLHADGVRVAQVL
                     SNLLNNAAKYTADGGHIWLSAAVENGRALISVRDNGRGIPTTMLNRVFDLFTQVDTSR
                     GTPGLGIGLAMVRMLVELHGGEVEARSTGPGHGSEFIVRLPMLTPESSAGTKSKAAGI
                     DTSLAGRSVLVVDDNCDAADSLSMLLRLAGAKVTVAYDGASALAAIPIHQPQVVLLDV
                     GMPGMDGYTVASRIRQDAQNSDMLLIALTGFGQEEDRKRSSAAGFNDHLTKPADLEAL
                     LSLVASFEPMAANQADLQKRPDLRKVR"
BASE COUNT          773 a         1164 c         1135 g          824 t
ORIGIN      
        1 tgagtcagaa aaaacggccc gaaacggcgt tcgagcaaca ggctgaactg gtttttgttt
       61 tccatggcgg gcagacgctc gattggctgc acagcttatg aaaagggcgc ccacaacgca
      121 acgtcgccgt ccttgcgcca accagccttc gctgcgactg cgggctcgaa cggcccctcg
      181 ccaaggcctt ggcattggct ggtctgatgg gtcgatcatg ccggatgaac cagccgcgcc
      241 ggctcagtgg aactcgtaat atggcccggc actcatatac gctagatcca tgccggatca
      301 acccttagga gatcatgatg acgaagacac tttccgcgct cgccgccggc ctgtttgcat
      361 tgacgcttgg gttcggcgcg cacgctgcca gtgaccaata caaggtggcg aaaaagcaag
      421 ccgacaccag ctacaaatcg gcgaaggccg aatgcaagaa aatgagcgga gatgaaaaga
      481 aaaactgcat gaacaaggcc aaagccgatc atcaggccgc cttggataga gccaaaacca
      541 tgaaatgaca gtaaccagct ttgcgccgta accgctcggg aatcaactca ccagagtggt
      601 tacggttgcg tcgaaactac ttgttgctgc gccggcttcg cgcacatagt ttgttgatcg
      661 ttcctcgctc acaggccagc acccggccgg gatcgagtcc cgcatccagg cctaatgtct
      721 ttagcgcctg cacgacttta tcgaaatttt caaaggtccc ggtcaatgcc aggtcgcggg
      781 cctgctgcca tacttcgatt ctcttttttc cttggatgat atccgggctg ctcttcattc
      841 tttagcgttc cagttgtttg ctcgtgtcgc cgttggtacc ggcctgcaat tgcattcatg
      901 caatttgcat tctcggttgc atgctatgcg caaggtcgac actgcggaac ggaccgaacg
      961 gtctatgaca gaccttgctt gccggcaaca tcattgaagt ggactcgtgc gggaccgggt
     1021 cgcggcgaac agaatcctgg gctgctatgc ggccggagag gctaacgcgt ctttttcaga
     1081 tcggccttgc ggcgtctggg aatctcggga tggctcgcaa ggcattccgg gcagattacg
     1141 ccggcgccgg gcgccaggcg ttccacgaaa ttccagaccg gccaaccctc acattccggc
     1201 gacagatgcc agacatcggt tccacgctcc cgtcgataca gtttttttct gcgttctatg
     1261 cccacgcccg aagaataaca ttccaggcgg caggcgcacc gggcacaaga tggcgccggc
     1321 gcattgcggg ctgactggag gtaaatggag cggattgagg gagtcgaacc atctatggca
     1381 gccgggagtg ggctgttacc cgccgtcgag taatccgcgt ggtgatcagc gcggcatgtt
     1441 ccaaaccttc ccgggtttcc cgcgaagtta caatgccgca ccgatcaagg gcgagtatag
     1501 agaggcggcg cttcagcatg aagcagcgag gtgcgccgtt catggcactt tcggcctatc
     1561 gtgcccgctc cgggacagtt gaacctattc ctgtcgactg tgtgtcagcg cacagaactt
     1621 cgaggcggtt cgtaagacac tgtcctgcct cttctgcatg agcacttccg gactcttcat
     1681 gctcatgtag tttgctcccc ggctcaatgc cggggttttc ttttaggcca ttccgatccg
     1741 atgaaaagct aaagcttggt ggaagccggc ctaaaaactt gcgctagcgc accttccgaa
     1801 gatcaggcct tttctgcaag tcggcctggt ttgctgccat gggttcgaac gatgcaacca
     1861 gggaaagcaa ggcctccagg tcggctggct tggtcaggtg gtcgttgaaa ccggcggcgc
     1921 tcgagcgttt gcgatcttct tcctgtccga agccggtcag tgcgatcagc agcatgtcac
     1981 tgttctgcgc atcctggcgt attcggcttg cgaccgtata gccgtccata cccggcatgc
     2041 cgacatcgag caataccact tgtggctgat gaatcgggat ggcagccagc gcgcttgctc
     2101 cgtcataggc tacggtcact ttggcacctg ccaggcgcag cagcatggaa aggctgtcgg
     2161 ccgcgtcgca attgtcgtcc accaccagga cgctgcgtcc cgccagggac gtgtcgatgc
     2221 cggctgcctt cgacttcgta ccggcactgg attccggcgt cagcatgggc aggcgcacga
     2281 tgaactcgct gccgtgaccg ggcccggtgc tgcgggcttc gacctccccg ccatgcaatt
     2341 cgaccagcat gcgtaccatc gcgagtccga taccgagtcc cggagtgccg cgactggtat
     2401 cgacctgcgt gaacagatca aacacgcggt tgagcatggt ggtcggaatg ccgcgaccgt
     2461 tgtcccgcac gctgatcagc gcgcgaccgt tttcgaccgc cgcgctgagc cagatgtgcc
     2521 cgccgtcagc agtatatttg gccgcattgt tcagcagatt cgacaaaacc tgggccacgc
     2581 gcacaccgtc ggcatgcagc atgaccggct ccgtgggcac ggacaggtcg agttgatggc
     2641 gtccagcgcc gatgagcggc tggctcatct caatggcatg gcgcaggacc gtttgcagtt
     2701 cgatcggcac gagcttgagt tcaatcttgc ccatcgtgta tcgcgagaca tccatgagat
     2761 cgtcgaccag acggatcata tggttggtct ggcgctcgag catggcatgg atctgtgtct
     2821 gttccgcgga ttcgaccgcc gattggcgca aaacggacaa gccgttgtag attggcgcca
     2881 ggggattgcg caactcgtgc gccagggtcg cgaggaattc atcctttctg cgatccgcct
     2941 cacgcagcgc tccctccagt ttcttgcgcg cggtgatatc catcatcacg ccaagatagg
     3001 tcgaccggct tgttgcgctt tcatgcgtac agtcgccgcg caagtgcatc cagcggattt
     3061 ctccgtcggg catctccacc cggaactcga attccatgaa cagcccatgc tcgatggagt
     3121 gcgcgtgtac acggcgccgg gtgaccacat cttccgggtg catcagcgct tccagagcct
     3181 caatcgttcc tgtccaatcg gccttgcgca cattgatcag atcgagaaat tcgtcggaac
     3241 aggcaagctt gccacttgca acatcgagct gccaggtacc caggcgcgcc gcctccgcgg
     3301 caagcttgat gagccgattg gcttcaagga gttgcgcctg cacctgcgca tgtttttttc
     3361 gcgcggcatc ctcacgtagg gcacgctgga ttgccggtgc caggcgagca agccggtctt
     3421 tcaatacgca gtccgtcgca ccgctgtgca gcagttcgat tgcaccctcg tcgcccaggg
     3481 cgccggtcac cagaatgaac ggcacgtgaa tcgccgcgct gcgaacatat tccagtgcct
     3541 ggagaccatt gaagcgcggc acgcgatagt cagacagaat gatgtcgaaa ttggtttttc
     3601 gaatctctgc ttcgaaggct tcgcgaccgg gggccacggt aatcaacacg tcgaggccgg
     3661 agcgcaacaa ctgctcgcgg atcaggcgcg cgtcggcagc atcatcctcg aggtgaagaa
     3721 tccgaatcgg agcgctcatg gcggtcttgc ctgtgatctg gattgcgaca gacaaccggg
     3781 cggcggctca tttgcccagc cccagaagcg cgccagctcg gcgtaacgtt ccgcgcgaac
     3841 attttggtct tcgtgattca gaaccagact gttcacgcct tgttcgtagg ccctgc
//
LOCUS       DATMZQ010000501         6920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3771317, whole genome shotgun sequence.
ACCESSION   DATMZQ010000501 DATMZQ010000000
VERSION     DATMZQ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771317"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VN496_15930"
     CDS             complement(<1..735)
                     /locus_tag="VN496_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF18499.1"
                     /translation="MNKKTGGEFSTRFFGAISTQLDIMVLVPPLEIWQTAHRRRKFSV
                     FHFIDPMLTPYELFVGLRYTRAKRRNHFISFISVTSMLGIALGVAALIVVLSVMNGFQ
                     KELRARILGVASHVQITGMSNGLRDWQRVAKQSLSNPEVIAAAPFVMAQGLISNHQTV
                     QGSIVRGILPSDEDKVADIGMHMKSGRLGALQAGKFGIVLGSELALSIGARLGDKIVV
                     IAPQGQVTPAGIMPRLKQFNVVGIFEV"
     gene            739..1749
                     /locus_tag="VN496_15935"
     CDS             739..1749
                     /locus_tag="VN496_15935"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18500.1"
                     /translation="MHTATLFIPALFWPHAGIAHDSPMAPALRTLLGRADYDEAICED
                     ENAWLCNQFGVTRQSDWPVAPIAMLGTALAPGDNFCLRADPVHLQVDRDRLILTVPET
                     LSISETEAATLVAALNRHFAADHFSFVAAEPHRWYLKTPRPARIVTRSLSRVVGSDID
                     RLLPEGDEKLAWHRIFNEVQMVLHEHQVNQERELRGVQPINSLWFSGGGILPHAKTDF
                     DAVIASSALGRGLAKLADIPLAPAEHAAELSARHVLIELCDAETASLRLDPEVWKHAI
                     EAMDERWFAELVNLLRTGRVRRLVICTVAAGRYLRWSISRRHLWRLWRSPASLAGPLR
                     KA"
     gene            1803..3461
                     /gene="recJ"
                     /locus_tag="VN496_15940"
     CDS             1803..3461
                     /gene="recJ"
                     /locus_tag="VN496_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXF18501.1"
                     /translation="MLQGLNPLLAKIYAGRGIDDISRLDTSLSRLLPYDQLLNCTTMA
                     SLLADAIAAEKRLLIVADYDADGATACAVGMLGLGAFGAKVEYLVPNRFEYGYGLTPE
                     IVTLAAARKPDVLITVDNGIASLEGVTEANRLGIRVLITDHHLPGEQLPAAACIVNPN
                     QPGCSFPSKHLAGVGVMFYVLVALRVELRRRNWFGQRKEPNLAELLDLVALGTVADVV
                     RLDDNNRILVHHGLQRIRAGRARTGIEALIGVAGRDVARVSAYDLGFVLGPRLNAAGR
                     LTDMSVGIECLLTQDQGKSMDLAKKLDMLNRERREIEAGMQQTAMALVDEIAIGDSFS
                     ISLYDPAWHQGVIGILAARLRERFHRPAIAFAPSGSGEAKGSGRSIPGLHLRDALDLV
                     SKRNPNLILRFGGHAAAAGLTVRTDQIAAFGIAFENVVRDLIGTAELERLVETDGGLP
                     PAEISLAFAETLEYPVWGQGFKAPTFGDTFDIAEQRLVGGKHLKLRLRAADSKRVFDG
                     ILFGQENLLPDRIFGVYGVQVNEFNSVRSVQLVLEHWQGVSGET"
     gene            3609..4713
                     /gene="prfB"
                     /locus_tag="VN496_15945"
     CDS             join(3609..3689,3691..4713)
                     /gene="prfB"
                     /locus_tag="VN496_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435212.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXF18502.1"
                     /translation="MEAEQLNALANQLDDLDLRSKELRRYLDFEGKRNRLSEVVHLAE
                     DPAIWNDAKRAQDIGRERKQLESVVDTLEKLDQGLRDARELFVMARAESDDSALRAIA
                     GDISDMEKAVAGMEFRRMFSNPMDPNNCFLDIQSGSGGTEAQDWASMLERMYLKFCDR
                     RGFQVEVLEESPGDVAGLKSAALKVTGEYAFGTLRTESGVHRLVRKSPFDSNARRHTS
                     FASVFVYPEIDDTIEIAINPADLRVDTFRASGAGGQHINKTDSAIRITHLPTNIVVQC
                     QSDRSQHRNRAEAMAMLKARLYELEMRKRNEEKQAMEDSKTDIGWGHQIRSYVLDQSR
                     IKDLRTNVERGDTQSVLDGDLDDFINASLKQGV"
     gene            4718..6223
                     /gene="lysS"
                     /locus_tag="VN496_15950"
     CDS             4718..6223
                     /gene="lysS"
                     /locus_tag="VN496_15950"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXF18503.1"
                     /translation="MEEKAKLAETVDTNQVIEERKAKLRALRSAGNAFPNDFRRTELA
                     AHLHQQYDAKSNDELEPAAIRVTIAGRMMLKRVMGKASFATLQDMSGRMQLYVSNDIA
                     GEAVHAAFKHWDLGDILGAEGTLFKTKTGELSVKVTRLRLLAKSLRPLPEKFHGLADQ
                     EQKYRQRYLDLITNPESQRVFKARSSIIQAIREFFVERGYLEVETPMMQPIPGGAAAR
                     PFVTHHNALDMQLYLRIAPELYLKRLVVGGIEKVFEINRNFRNEGMSTRHNPEFTMLE
                     FYEAYQDYHYLMDLTEELLRKLAHKVNGSTTLNYQGQAIEFGKQFDRFTMAEAIARYN
                     SQYELQDLSSIEMLRGALKHFDIEVFPTDGLGILQLKLFEETTESKLIQPTFIYAHPV
                     DVSPLARSNDQNPAVTDRFELFITGRELANGFSELNDPEDQAARFQRQVLAKEAGDEE
                     AMYYDADYVRALEYGLPPTAGEGIGIDRLVMLFTDSASIRDVILFPQMRPE"
     gene            6294..>6920
                     /locus_tag="VN496_15955"
     CDS             6294..>6920
                     /locus_tag="VN496_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXF18504.1"
                     /translation="MAANPEQLWQPSTSRIADANITAFIKLINKRWQAGVADSIALYE
                     WSVAQPEQFWTAVWDFCGVIAERRGERVLVDGHKMPGARWFPDAKLNFAENLLRRRDD
                     ETAMVFWGENKVRRRMSFFELHDAVSRTAQALTAAGVNQGDRVAAFMPNLPESIIFML
                     AAASLGAIWSSCSPDFGVQGVLDRFGQIKPTILFAVEGYHYNGKVIDTL"
BASE COUNT         1568 a         1926 c         1970 g         1456 t
ORIGIN      
        1 gacttcgaat atcccaacta cattgaactg tttgaggcgc ggcatgattc cagctggcgt
       61 cacttggccc tgtggcgcaa taacgacgat cttgtcgcca aggcgggcgc cgatgctgag
      121 cgccagttca ctacccaaaa cgataccgaa ttttcccgct tgcagtgcac cgagtctgcc
      181 cgatttcatg tgcatgccaa tgtcggcaac cttgtcttca tccgacggca aaatgccgcg
      241 cacgatactg ccctgcacgg tctggtgatt tgatatgagg ccttgcgcca taacaaacgg
      301 cgccgctgcg ataacctccg ggttgctcag tgattgtttg gccacccgct gccaatcccg
      361 gaggccattg gacatgcccg taatttgcac atgggacgcg acaccaagaa tgcgagcccg
      421 cagctccttc tggaaaccat tcatgaccga cagcacgaca atcagcgccg caactcccaa
      481 ggcgataccg agcatggaag taacggaaat gaaggagatg aaatggttgc gccgcttggc
      541 gcgcgtatag cgcaggccga caaacagctc ataaggcgtc aacatgggat cgatgaaatg
      601 aaagaccgaa aatttgcgtc ggcgatgcgc agtctgccat atttccagag gtgggactaa
      661 caccataatg tccagctgtg ttgagatggc gccaaaaaaa cgcgtgctaa actcaccgcc
      721 ggtctttttg ttcacgccgt gcacactgcg actttgttca tccccgcatt attttggccc
      781 catgcaggca tcgcgcacga ttctccgatg gcgcctgcat tgcggacatt gctgggtcgc
      841 gccgactatg acgaagcgat ctgcgaggac gaaaacgcct ggctatgcaa tcaatttggg
      901 gtaacccggc agtctgactg gccggtcgcg cccatagcaa tgttgggcac ggcgttggct
      961 cctggagaca atttctgttt gcgtgcagac ccggtgcatt tgcaggtcga tcgcgatcgg
     1021 ctcatcctga ctgtccctga aacgctgtcg atttccgaaa cagaggcggc cactctggtt
     1081 gcagcgctta atcgccattt cgcagccgac catttcagct tcgtggcggc ggagcctcat
     1141 cggtggtatc tcaagacccc tcgtcctgcg cgtattgtga ctcgcagctt gtcgcgcgtc
     1201 gtcggcagcg atatcgatcg tctgctaccg gaaggggacg aaaagcttgc atggcacagg
     1261 atatttaatg aagtgcaaat ggtgctgcat gagcatcagg tcaaccagga acgtgagtta
     1321 cgcggtgtgc agccgattaa cagcctatgg ttttcaggcg gcggtatatt gccccatgca
     1381 aagaccgatt tcgatgcggt gatagcgagc agcgcgctcg ggcgtggact ggcgaaacta
     1441 gcggacatcc cgcttgcacc ggccgagcac gcggccgagc tcagcgcaag gcatgtactc
     1501 attgaactgt gcgatgctga aacggcatcc ctgcgcctcg atccggaagt ctggaagcac
     1561 gcgattgagg cgatggacga gcgttggttt gcagagctgg ttaatttgct gcgcactgga
     1621 cgtgtgcgcc ggcttgtcat ttgtacagtc gccgcaggac ggtatttacg ctggtcgatc
     1681 agcagaaggc atctatggcg actgtggaga tcgccggcgt cgctggcagg ccccctccgc
     1741 aaagcatgaa tccttctcgc attgccgcgc gcgtggtgcc gcctgcagct tttgacaagc
     1801 tgatgctcca ggggctgaat ccgctgctgg ccaaaatcta tgctggccga ggcatagacg
     1861 acatttccag gctcgataca tcgctgagcc ggctgctgcc ctacgatcaa ttgctgaact
     1921 gcacgaccat ggccagtcta ttggcagatg ccattgcagc ggaaaagcgc ctcctcattg
     1981 ttgccgacta tgacgcggac ggcgctacag cttgcgcggt cggcatgctg ggactcggcg
     2041 cctttggggc aaaagtcgaa tatctggtgc caaaccgctt cgagtacggc tatggactca
     2101 cgccagaaat cgtcaccctg gcggctgccc gcaagccgga tgttctcatc acggtggaca
     2161 atggtatcgc gagccttgaa ggcgttacgg aagccaaccg actcggcatt cgcgtcctga
     2221 tcacagatca tcatcttccc ggggagcagt tgcccgccgc cgcgtgcatt gtcaatccga
     2281 atcagcctgg atgttctttt ccgagtaaac accttgccgg cgtgggcgta atgttctacg
     2341 tactcgttgc tttgcgtgtc gaattacggc gccggaactg gttcggccag cgcaaggaac
     2401 cgaaccttgc cgaattgctg gatttggtcg cgctcggcac cgtcgccgac gtcgtgcgcc
     2461 tggacgacaa taatcgcatc ttggtacatc acggtctgca acgcatacga gcgggtcgag
     2521 cgcgcactgg tatcgaagcg ttgatcggtg ttgctggacg cgacgtagcc cgcgtgtccg
     2581 cttatgattt gggctttgtc ctgggtccgc gactgaacgc tgctggccgg ctcaccgaca
     2641 tgtcggtcgg aattgaatgc ctgctgacac aggatcaggg caaatcaatg gacctcgcca
     2701 aaaagcttga tatgctgaac cgcgaaaggc gcgagatcga ggcgggcatg cagcagaccg
     2761 cgatggcgct ggtggacgag atcgccattg gtgacagttt cagtatcagc ctctacgatc
     2821 ccgcctggca tcaaggcgtg atcggcatcc tcgctgcacg gctgcgcgag cgttttcacc
     2881 gtcctgcaat tgcatttgcg ccgtccggta gtggggaagc caagggttct ggacgctcta
     2941 taccggggct ccatttgcgg gatgcactgg acctggtgag caaacgcaat ccaaatctta
     3001 ttctgcgctt cggtggccac gcagcggcgg ctggactgac cgtgcgcacc gatcaaatcg
     3061 ccgcattcgg cattgcgttt gaaaatgtag tccgcgatct tatcggaacc gctgaactgg
     3121 aacgcctcgt cgagaccgat ggtggcctac cgccggccga aatctcactg gcctttgccg
     3181 aaacgctgga atatccggta tggggacaag gcttcaaggc cccgacgttc ggcgatacat
     3241 ttgacattgc cgagcagcga ctcgttggag gcaagcatct caaactgcgc ctgcgggcgg
     3301 ccgattcgaa acgtgtcttc gatggcatcc tgtttggcca ggagaatctg ttgcccgatc
     3361 gcatctttgg cgtctatggc gtccaggtga acgaattcaa ctccgttcgc tcggtgcaac
     3421 tagtgctgga acattggcaa ggcgtctccg gagaaaccta gtagcccaag cttcccggaa
     3481 accgaggatt cccgcgcgaa cgccataccg atccagggcc gcttttgcca atcagacagg
     3541 cggcttgcaa agccataagc taaaatgcgc gcctttcacg cctcgctatt gatcaaatcg
     3601 gactccatat ggaagcggaa cagcttaatg cgttagccaa ccagctcgac gatctggatt
     3661 tacgtagcaa agaactgcgg aggtatcttt gacttcgaag gtaaacgcaa tcgtctgagc
     3721 gaggtcgtcc acctggcaga agatcctgca atctggaacg atgccaagcg tgcccaagac
     3781 atcggccggg aacgcaagca acttgagtcc gttgtcgaca cgctggaaaa gctggatcag
     3841 ggactgcgtg atgcgcgcga actttttgtt atggcgcgcg ccgaaagcga cgattctgcg
     3901 ctgagggcca ttgcaggaga catctccgac atggaaaaag cagtggcagg aatggaattc
     3961 cgccgcatgt tttccaaccc catggatcca aacaattgct ttcttgatat acagtctggc
     4021 tccggaggga ccgaagcaca ggactgggct tcgatgcttg aacgcatgta tctgaaattc
     4081 tgcgaccggc ggggcttcca ggtcgaggta cttgaagaat cgcccggtga cgtggccgga
     4141 ctgaagagtg ccgcgctgaa agtaaccggc gaatatgctt tcggcacatt acgcacggaa
     4201 agcggcgtgc atcgcctggt acggaagtca cctttcgatt ccaatgcacg acgccataca
     4261 tcgttcgcca gcgtattcgt ttacccggaa atagacgata cgatcgaaat tgcgatcaat
     4321 ccggcggacc tgcgcgtcga tacgtttcgt gcaagcggtg ccggcggcca gcacatcaac
     4381 aaaacagact ctgcaatccg catcactcat ctgccaacga atattgtcgt gcagtgccag
     4441 agcgaccgat ctcagcaccg caatcgcgcg gaggccatgg cgatgcttaa agcccggttg
     4501 tacgagttgg agatgcgcaa gcgcaacgaa gaaaagcaag ccatggaaga ttccaagact
     4561 gacatcggct ggggccacca aatccgttcg tacgtgctcg atcagtcgcg catcaaggat
     4621 ttgcgcacca acgtagaaag aggtgatacg cagtccgttc tggatggcga cctcgatgac
     4681 tttattaatg ccagccttaa gcaaggcgta tagcaaaatg gaagaaaagg caaagcttgc
     4741 agagaccgtt gataccaacc aggtcattga agagcgaaaa gcaaaactcc gggctcttcg
     4801 cagtgcaggc aacgcgtttc ccaatgactt ccgcagaacc gagctcgccg cgcacttaca
     4861 tcagcaatac gacgccaagt caaacgacga acttgaacca gccgccatcc gggtaacgat
     4921 agccggacgc atgatgctca agcgtgtcat gggcaaagcc agctttgcca cactgcagga
     4981 tatgtcgggc cggatgcagc tgtatgtcag caacgatatc gcgggcgaag cggtgcatgc
     5041 ggcattcaag cattgggacc tgggagatat tctcggcgcc gaagggacgc tgttcaagac
     5101 caagaccggc gaactgagcg tcaaggtcac caggctcagg ctgttggcga aatcactgcg
     5161 gccgcttccc gaaaagtttc atgggcttgc cgaccaggaa caaaagtacc gccagcgcta
     5221 tctggacctg attaccaacc cggaatcgca acgggtattc aaggcgcgct ccagcattat
     5281 ccaagcaatt cgcgagtttt tcgtggagcg cggctatctc gaagtggaaa cgccgatgat
     5341 gcagcccatt ccgggcggtg ccgcagcgcg cccgtttgtg acgcatcaca atgcgctcga
     5401 catgcaactg tatttacgca tcgccccgga gctttatctg aagcgcctcg tggtcggcgg
     5461 cattgagaaa gtgttcgaaa tcaaccgcaa tttccggaat gaaggcatgt cgacgcggca
     5521 caatccggag ttcaccatgc ttgaatttta tgaagcctat caggattacc attatttgat
     5581 ggacttgacg gaagaattgc tccggaaact cgctcacaag gtaaacggca gcacgaccct
     5641 aaactaccag ggccaggcga tcgaatttgg caaacaattt gatcgcttca ccatggctga
     5701 ggccattgcc cggtacaact cccagtatga actgcaggat ctctcaagca tcgaaatgct
     5761 acgtggcgcc ttgaagcatt tcgacattga agtgtttcca actgacgggt tgggcattct
     5821 gcaactcaag ctgttcgagg aaacgacgga aagcaaattg atccagccca cgttcatcta
     5881 cgcccaccct gtcgacgtgt cgccgttggc gcgtagcaat gatcaaaatc cggccgtgac
     5941 cgaccgcttc gagttgttca tcactggacg cgagttggcg aacggcttct ccgagctgaa
     6001 cgacccggaa gaccaggccg cacgttttca gcggcaggtt ctagccaaag aggctggaga
     6061 cgaggaagcg atgtattacg acgccgatta tgtgcgggcg ctggaatatg ggttgccgcc
     6121 taccgccggc gaaggcatag gtattgaccg actggtgatg ctgttcaccg atagcgccag
     6181 tatccgggat gtcatcctgt ttccacagat gcgccctgaa tagagttggt tcaacagcct
     6241 ggaacaaacc tgcgggttcc attgactaga atgcatttgc aagctgagtg atcatggcag
     6301 caaaccctga acaactctgg cagccgtcta ccagtcgcat cgccgatgcg aatatcaccg
     6361 ccttcatcaa gctgattaac aaacgctggc aggccggtgt tgctgattcc atcgccctct
     6421 atgaatggtc ggtcgcccag ccggagcaat tctggacggc ggtatgggac ttttgcggcg
     6481 taattgcaga acggcgcggt gaacgtgttc tggtcgatgg tcacaaaatg cccggtgcgc
     6541 gctggttccc tgacgccaaa ctcaattttg cggaaaacct gctgcgcagg cgtgacgatg
     6601 aaactgcgat ggtgttctgg ggcgagaaca aggtccgtcg cagaatgagt tttttcgaac
     6661 ttcacgacgc agtgtcccgt acagcgcagg cgctaactgc agcgggcgtc aaccaagggg
     6721 atcgtgtcgc ggccttcatg ccgaacctgc ccgaaagcat tatttttatg ctggccgcgg
     6781 ccagccttgg tgcgatatgg tcgtcctgct cgcccgattt tggcgtacag ggcgtcctcg
     6841 accgatttgg ccaaatcaaa ccgaccatcc tgttcgcggt tgagggctac cactacaacg
     6901 gcaaggtaat cgatacctta
//
LOCUS       DATMZQ010000502         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4813458, whole genome shotgun sequence.
ACCESSION   DATMZQ010000502 DATMZQ010000000
VERSION     DATMZQ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4813458"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..860)
                     /gene="hpnC"
                     /locus_tag="VN496_15960"
     CDS             complement(45..860)
                     /gene="hpnC"
                     /locus_tag="VN496_15960"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HXF18505.1"
                     /translation="MAQNHYENFPVASVLLPARLRRPVQLIYAFARQADDFADEGNWP
                     AQERLAKLSGFARQLDLIEAGKTPSVQLFVDLAQVIEQFKLPVGLFRDLLSAFAQDVV
                     KTRYASVGELMDYCRRSANPVGRLMLHLYRATAPEHFVWSDDICSSLQLINFWQDVGI
                     DFSKGRIYLPADEMAEYSVTETQISNRDANSNWRNLMLFQIDRTQRMLRAGAPLGRAL
                     PGRIGLELRMIVMGGNRVLEKLRAAGGDVFRDRPVLRTFDWPVMLYRSLIAYP"
     gene            961..1045
                     /locus_tag="VN496_15965"
     tRNA            961..1045
                     /locus_tag="VN496_15965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:995..997,aa:Leu,seq:tag)
     gene            1104..2411
                     /gene="tig"
                     /locus_tag="VN496_15970"
     CDS             1104..2411
                     /gene="tig"
                     /locus_tag="VN496_15970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXF18506.1"
                     /translation="MQANLETLSSLERRLSVTLPLDKVNTEVASRLKRLAATVKLHGF
                     RPGKVPMSIVEQQFGGQVRQEVLGDAVQRSFGEAVKEKNLRVAGYPRIEVKPGDMPTE
                     KLEYIATFEVYPEVSLGELAQIKIERPVVDINDAEVDKTVEILRKQRTHYHAVERCAR
                     QGDQVRIDYRGTIAGQAFDGGSGTDQNVLLGGGQLLADFEKNLIGMQAGEQREFDLRF
                     PDDYHGKQVAGKDAHFEVQLKEVQEPHVPEANAEFAKALGIADGDLTKMRADVKANLE
                     REVKTRANKRVKDQVMKALLDATKVEVPRALLDMEVERLMQNMRHDLEQRGLKSDQIP
                     MPKEAFEPEAVRRVSLGLILAELVKLHQLNAKPEQIKAVVQEYAQSYEKPEEVVRWYY
                     QQPERLREVESIVLEDNVVQWTLSNAKVEAKPTDFDDLMGNAQ"
     gene            2408..3061
                     /gene="clpP"
                     /locus_tag="VN496_15975"
     CDS             2408..3061
                     /gene="clpP"
                     /locus_tag="VN496_15975"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXF18507.1"
                     /translation="MMTMHDRFYAEPQALGLVPMVIEQSGRGERAYDIYSRLLKERVV
                     FLVGPVTAMTANLIVAQLLFLESENPEKDISFYINSPGGSVSAGLAVYDTMQFVKPDV
                     STLCVGQAASMGAFLLAAGTKNKRFALPNSRVMIHQPMGGFQGQASDIEIHAREILYL
                     RGRLNELMAKHTGQSVETIERDTDRDNFLSAEDSVKYGLVDKVLLSRSETAAAAAAA"
     gene            3132..>3688
                     /locus_tag="VN496_15980"
     CDS             3132..>3688
                     /locus_tag="VN496_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HXF18508.1"
                     /translation="MAEKPGGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREESHAETTGKGVKSDLPVPHEIRESLDQYVIGQDIAKKILSVAVYNHYKRLKTVQ
                     KKDDVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVENI
                     IQKLLQKCDYDVEKAQRGIVYIDEI"
BASE COUNT          907 a          959 c         1083 g          739 t
ORIGIN      
        1 ggaaccgctc cttgcggctt tatcctgaca atattcctct ggcgtcatgg gtacgcaatc
       61 aaggagcggt aaagcattac gggccagtcg aacgttcgaa gtaccggacg atcccggaac
      121 acgtcgccgc cagccgcccg caatttttcc aatacgcgat ttccacccat tacgatcatg
      181 cgcaattcca gcccaatgcg tccaggcagc gcacggccga gcggcgcacc ggcccggagc
      241 attcgctgcg tgcggtcgat ctggaatagc atcagattgc gccagttgct gttggcatcc
      301 cggtttgaga tttgggtttc ggttaccgag tattctgcca tttcatcagc cggcagatag
      361 atcctgccct tgctgaagtc gatcccgacg tcctgccaga aattgatcaa ttgcaggctt
      421 gagcagatgt catcggacca gacgaagtgc tccggtgcgg tagcgcgata cagatgcagc
      481 atcaaacgtc ccaccggatt ggcggaccgc ctgcaataat ccatcaattc cccaacactc
      541 gcataacgtg tcttgacaac atcctgggca aacgcagaga gcaagtcccg gaacaaaccg
      601 accggaagct tgaactgctc aatgacctgc gcgaggtcaa cgaacaactg aacggaaggc
      661 gtctttccgg cttcgatcag atcgagttgc cgcgcaaaac ctgacagctt cgcgagtctc
      721 tcctgcgcgg gccaatttcc ttcgtcggca aaatcatcgg cctggcgggc aaaagcgtag
      781 atcaattgga ccgggcgccg cagtcgcgcc ggaagcagaa ccgaagcgac gggaaaattt
      841 tcataatgat tctgcgccat cgaattgatc agcagtacga gaagtgggct ctgcaatggc
      901 accggtggcc gaggcgcatt ggatgacggc gcgggggcat tatattagaa tgcagccagc
      961 gcgaaagtgg cggaattggt agacgcacca gatttaggtt ctggcgccga gaggcgtgag
     1021 agttcgagtc tctcctttcg caccagaacc gcgcgaaagt cccggcaatt tttctacccc
     1081 accaaccgga agaacggaat ttgatgcaag ccaacctgga aaccctcagt tcactggaaa
     1141 gacgcctgtc agttacgttg ccgctcgaca aggtcaacac tgaagtcgcg tcccggttga
     1201 agcgcctcgc cgccaccgta aagcttcacg ggttccgtcc ggggaaagtg ccgatgagca
     1261 tcgtggagca gcagttcggc gggcaggtga ggcaggaagt gctcggagat gcagtgcagc
     1321 gtagcttcgg agaggcggtc aaggaaaaaa acttgcgtgt cgcgggctac ccacgcattg
     1381 aagtcaagcc gggcgacatg cccacggaaa agcttgaata tatcgccacg tttgaagtct
     1441 atcccgaagt ttcgctcggc gaactggccc aaatcaaaat tgagcgccca gtggtcgata
     1501 tcaatgacgc agaagtggac aagacggttg agatcctgcg caagcagcgc acccactatc
     1561 atgcggtcga gcgctgcgcc cgtcagggcg accaggtacg catcgactac cgcggcacaa
     1621 ttgcgggcca ggcctttgat ggcggtagtg gcacagacca gaatgttttg cttggcggag
     1681 gtcagttgct cgccgacttc gagaaaaacc ttatcggcat gcaggccggt gaacagcgtg
     1741 aattcgattt acgctttcct gacgactacc atggcaagca agtagcgggg aaagatgcgc
     1801 attttgaagt gcagttgaag gaagttcagg agccgcatgt acccgaagcg aatgccgagt
     1861 ttgccaaggc cctgggtatt gccgatggcg acctgaccaa aatgcgggca gacgtgaaag
     1921 ccaatctcga gcgtgaggtc aaaacgcggg ctaacaagcg agtcaaagac caggtcatga
     1981 aggcgctgct cgatgcgacc aaggttgaag taccgcgagc tcttctggac atggaagttg
     2041 agcggttgat gcagaacatg cgtcacgatc tggaacaaag aggccttaag tccgaccaga
     2101 ttcctatgcc aaaagaggca ttcgagccag aggccgttcg gcgtgtgagc ctgggattga
     2161 ttctggcgga gttggtgaaa ctgcatcaac ttaacgccaa gccggagcaa ataaaggccg
     2221 tcgtgcagga atacgcgcaa agttatgaga aaccggaaga ggtggtgcgg tggtattacc
     2281 agcaacccga gcggcttcgt gaagtcgaat caatcgtgct ggaggacaac gtagtgcaat
     2341 ggacgctgtc gaacgcaaag gtagaagcaa agccgacgga tttcgacgac ttaatgggga
     2401 atgcacaatg atgactatgc atgatcgttt ctatgccgaa ccacaggcac tgggcctggt
     2461 gcccatggtt atcgagcaaa gcggccgcgg ggaacgcgcc tatgacattt actctcgcct
     2521 gctcaaggag cgtgtcgtat ttctggtcgg tcccgttacc gcgatgacgg cgaatctgat
     2581 cgtggcgcag ctgctttttc ttgaatccga aaatccagag aaggacatat ccttttacat
     2641 caattctccg ggcgggtcgg tatccgcggg actagccgtg tacgacacca tgcaattcgt
     2701 aaaacctgat gtcagtacgt tatgcgttgg tcaagccgcc agcatgggag cgttcctgct
     2761 tgccgccgga accaagaaca aacgttttgc tctgcccaat tcccgagtca tgattcacca
     2821 gccgatgggc gggtttcagg gacaggcttc ggatattgag atccacgcgc gcgagattct
     2881 ctatctgcgc ggccgtctca atgaattgat ggcaaagcat acggggcaga gtgttgagac
     2941 cattgagcgc gatactgacc gcgataactt tctaagcgct gaagattcgg taaagtacgg
     3001 tttggttgac aaggtgttgc tgagccgcag tgaaactgca gctgccgcgg cggcggcgta
     3061 agttgggcgc ggggtacgcc ccgaagggtg gctgtttggc ggcaaatacc atggcttgac
     3121 gaaagggaaa tatggctgag aaacctggtg gtgagaagct tctctactgc tccttctgtg
     3181 gcaagagcca gcacgaggtc cgcaagctca tcgccggtcc gtcggttttc atctgtgacg
     3241 aatgcattga actgtgcaac gacattatcc gcgaggaaag ccacgcggag acgacgggga
     3301 agggcgtcaa atcagacctt ccagtcccgc acgaaattcg ggaaagcctc gatcagtacg
     3361 tcattggtca ggacatcgcc aaaaaaatcc tgtcggtggc ggtgtacaac cactacaaac
     3421 gactgaagac agtgcagaag aaagacgacg tcgaactcgc gaagagcaat attcttctga
     3481 ttgggcctac cggctcgggc aagacgctgc tggctcaaac gcttgcgcgt ttgctcaatg
     3541 tgccgttcgt gatggccgat gcaacaaccc tgacagaggc gggttatgtc ggtgaagacg
     3601 ttgagaacat catccagaag ctgctgcaga agtgtgacta cgatgtcgaa aaggcccagc
     3661 gtggcattgt ctatatcgat gaaatcga
//
LOCUS       DATMZQ010000503         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4467314, whole genome shotgun sequence.
ACCESSION   DATMZQ010000503 DATMZQ010000000
VERSION     DATMZQ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467314"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..>2127
                     /locus_tag="VN496_15985"
     CDS             27..>2127
                     /locus_tag="VN496_15985"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HXF18509.1"
                     /translation="MIFVDRVNADDNLSYCENIEATNPCVTADTWIMTTDGPRQVAEL
                     VGRSFSAVVDGQVFESGQEGFFSSGKKPVVEVTTDEGLTLRLTADHKVLRVKRMTRFV
                     RETGWTEAAELCMGDHIVLHNHRAFSGWDGFGNEQQGYLVGLLVGDGTLKDDTAVLSV
                     WGSQKEVANGGQVFSLGSVSVMRAVHNAAKVFNQRADHGGWAKISDRNEYRFKLAALR
                     DLALDLEMQVGAKDITPAIERTSSKFYHGFLRGFFDADGSVQGDQRKGVSIRLSQSDL
                     PRLQVVQRMMLRLGIVSTIYADRRQGGPRVLPNGKGGSREYYCLAQHELIITGDNLVR
                     FADLIGFSDQEKSRRLVELLSAYRRAPNAERFVAAVKSVTVTGEREVFDAKIEGIRAF
                     DANGLYVHNCGEQPLPAYGCCCLGSVNLAPFVSGAFTQQASFDFDAFAKVVKVAVRML
                     DNVLDVTVWPLPQQAEEAKNKRRIGLGFTGLGDALIMLGLRYDTEEARSMGARIAEAM
                     RDTSYAASAELAREKGSFPSLDARRYLDAPRFASRLPEHIKQEIRATGIRNSHLLSIA
                     PTGTISLAFADNAANGIEPPYSWTYQRKKREADGSHKVYDVEDHAWRLYRHMGGDVAK
                     LPSQFVTALEIAALDHMRMVSVIAPFVDSAISKTVNVPEDYPFEDFRDLYFEAWKAGL
                     KGITTYRPNGVLGSVLSV"
BASE COUNT          448 a          562 c          661 g          456 t
ORIGIN      
        1 cgacttacga tcatgcagag cccggcgtga ttttcgtcga ccgtgtgaat gcggacgaca
       61 acctttcgta ttgcgaaaac atagaggcca caaatccctg tgttacggcg gatacctgga
      121 tcatgaccac agacgggccg cgacaggtag ctgaactcgt cgggcgatcg ttttctgcgg
      181 tggtcgacgg gcaagttttt gagtccggtc aggaaggatt tttcagttcg ggaaagaagc
      241 cagtcgtcga agtgactaca gacgagggcc tgacgttgcg cctgaccgcc gaccacaagg
      301 tgctgcgggt caagcgcatg acacgcttcg tgcgtgaaac cggatggact gaagcggccg
      361 agctttgcat gggtgatcac atcgtcttgc ataaccatcg tgctttctcc ggctgggatg
      421 gattcggtaa cgagcagcag ggatatctgg tggggttgct ggtcggtgac ggcacgctta
      481 aagacgacac tgcggtgctt tccgtatggg ggtctcaaaa agaggtagcc aacggcggac
      541 aagtattctc cctgggatcg gtttcggtca tgcgtgctgt tcataacgct gccaaagtct
      601 tcaaccagcg agccgaccat ggtggctggg ccaaaatctc ggatcgcaac gaatatcgct
      661 tcaagctcgc tgcgttgagg gacctcgccc tggacttgga gatgcaggtc ggggcgaaag
      721 atatcacgcc ggcaatcgaa cggacgtcct caaagttcta ccatggattc ctgcgcggct
      781 ttttcgacgc tgatggctcc gtccaaggcg atcagaggaa gggagtaagc atcaggcttt
      841 cgcaatccga tctgccgcgt ttgcaggtgg ttcagcgaat gatgctgcgg ctcggcattg
      901 tatcaacgat ttatgcagac cgccgtcagg gaggaccgcg tgttttaccc aatggcaaag
      961 gcggaagccg ggagtattac tgcctcgcac agcatgaatt gatcattacc ggggacaacc
     1021 tcgtcagatt cgccgacctt atcggtttct cggaccagga aaagagccgg cggcttgtgg
     1081 agctattgtc ggcctatcga cgggctccga acgcggagcg cttcgtcgcc gcggttaaaa
     1141 gcgtgacggt tactggcgag cgcgaagtgt ttgacgcgaa aatcgaaggc attcgtgcct
     1201 tcgacgcaaa tggactctat gtccacaatt gcggcgagca gccgctgcct gcgtacggtt
     1261 gttgctgcct gggcagcgtg aatcttgcgc cgttcgtgag cggtgcgttc acgcagcaag
     1321 ccagttttga cttcgacgct ttcgccaagg tcgtgaaagt ggccgtgcgc atgttggaca
     1381 acgtgctcga tgtcactgtg tggccgctgc cgcagcaggc cgaagaagcg aagaacaagc
     1441 gacgcatcgg gcttggcttc accgggctgg gcgacgcgct gatcatgctg ggtttgcgct
     1501 atgacaccga ggaagcgcgc agcatggggg caagaattgc agaagcgatg cgcgatactt
     1561 cttatgcggc ctcagcggaa ttggcacgcg agaaaggcag ctttccgtca ttagatgccc
     1621 ggcgttatct ggacgcgccg cgttttgcct cgcgcttgcc ggaacatatc aagcaggaga
     1681 ttcgtgccac tggcatacgt aatagccatc tgctgtcaat cgcgccgacc ggcaccattt
     1741 cgctggcgtt tgccgacaac gcggccaacg gcattgagcc gccgtattcc tggacctacc
     1801 agcgcaagaa gcgtgaggcg gacggttcgc acaaggttta cgacgtggaa gatcatgcct
     1861 ggcgcctgta ccgccatatg ggcggagacg ttgcaaagct gccgtcgcag ttcgtgactg
     1921 cgctcgaaat tgcggcgctg gaccatatgc gcatggtctc agtcattgcg ccattcgtgg
     1981 attccgccat cagcaagacg gtgaatgtgc cggaagatta tccattcgaa gatttcaggg
     2041 atctgtattt cgaagcgtgg aaggccggac tgaaaggcat tacaacctac cgcccgaacg
     2101 gcgtactcgg ctcggtgctg tccgtta
//
LOCUS       DATMZQ010000504        16709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1018748, whole genome shotgun sequence.
ACCESSION   DATMZQ010000504 DATMZQ010000000
VERSION     DATMZQ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018748"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VN496_15990"
     CDS             complement(<1..771)
                     /locus_tag="VN496_15990"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXF18510.1"
                     /translation="MTDLANVAALTRTPVQLPIEWYFDPEIAEIERKVLFENGPGYVG
                     HELMVPEVGNYRSLDWMSNAKVLVRSQNGVELLSNVCRHRQSIMLKGSGHTHNIVCPL
                     HRWTYDLSGKLLGAPHFPGNPCLDLGKTGLTGWNGLQFAGPRDPNLDLARLGVLADLD
                     FSGYRLDRIVRHEYSVNWKTFIEVYLEDYHVAPFHPGLGQFVNADDLKWEFGDWYSVQ
                     TVGINQALGKPGTEAYQKWHEAVLRYNNGEMPKQGAIWL"
     gene            995..1252
                     /locus_tag="VN496_15995"
     CDS             995..1252
                     /locus_tag="VN496_15995"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXF18511.1"
                     /translation="MTKSKSQSPLSFQAPPSFEAALAEIENIVARMEAGQLPLDESLL
                     AYKRGAELLQYCQARLQEAQQQVKVLEAGTLQNLDGDDSAE"
     gene            1266..2135
                     /locus_tag="VN496_16000"
     CDS             1266..2135
                     /locus_tag="VN496_16000"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXF18512.1"
                     /translation="MLDRQARIETTLQSALPASEIAPARLHSAMRYAVLGAGKRVRPL
                     LAYGAGELACAQIERVDYAAASVELIHAYSLVHDDMPSMDNDILRRGRPTVHVEFDEA
                     TALLVGDSLQTLAFQLLSERAVADDASRQLEMIALLARASGSRGMAGGQAIDLESTSQ
                     ALSVPELEYMHIHKTGALIRASVALGARCGGALSDAQLHQLDHFGKFIGLAFQVVDDV
                     LDAQSTTATLGKTAGKDARNQKPTYVSALGIERSRALAGELRDNALQALDWLPDGGGH
                     LRQLADFIVLRKF"
     gene            2176..4020
                     /gene="dxs"
                     /locus_tag="VN496_16005"
     CDS             2176..4020
                     /gene="dxs"
                     /locus_tag="VN496_16005"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXF18513.1"
                     /translation="MYELLNTIQSPSELRALERKQLPELARQLREFLLESVAKTGGHL
                     SSNLGTVELTIALHYVFNTPQDRLVWDVGHQTYAHKILTGRRDAMSTLRQYGGIGGFP
                     RRSESEFDTFGTAHSSTSISAALGMAVAAHMKGENRRVVAIIGDGAMTAGMAFEALNN
                     AGAMDANLLVVLNDNDMSISENVGALNNYLARLISGRFYAAAWKTGEKVLRVVPPVLQ
                     LAKRAEEHVKGMMTPGTLFEEFGFNYIGPIDGHNLDVLVTTLGNIKKLDGPQFLHVVT
                     RKGKGYQQAEGDPILYHGVTKFEPTLGIAPKGASKPTYTQVFGDWLCDMAARDSRLIG
                     ITPAMREGSGLVRFSQEYPDRYFDVGIAEQHALTFAAGLACEGFKPVVAIYSTFLQRA
                     YDQLIHDICIQNLPVLFGIDRAGLVGADGPTHAGSFDLSYLRCLPNMVIMVPSDENEC
                     RQMLYTGFKLDGPVAVRYPRGSGPGVAIEPEMIELPVGKGEIRRRGSKLAILCFGALL
                     KPCMEAGEKLGATVANMRFVKPLDEELIRELARDHEHLVTVEENTVLGGAGSAVLEVL
                     EKRGVSRRTLQLGLPDRFIDHGDPAKLLQDCGLDANGIFHSIAAFIAD"
     gene            4089..4889
                     /gene="folE2"
                     /locus_tag="VN496_16010"
     CDS             4089..4889
                     /gene="folE2"
                     /locus_tag="VN496_16010"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HXF18514.1"
                     /translation="MNSPDNFTIPDVQGSADTRRLAIDKVGIKSIRHPVRVGDRDGGV
                     QHTIATFNMYVGLPHNFKGTHMSRFVEILNAHEREISVESFEAMLRDMVKRLEAEFGR
                     IEMTFPYFINKAAPVSGVQSLMDYEITFAGELSGAGYSFTMKAVVPVTSLCPCSKQIS
                     EYGAHNQRSHVTITARTNSHVWIEELVRVAEQEASCELYGLLKRPDEKYVTERAYDNP
                     KFVEDLVRDVATALNRDSRIDAYVVESENFESIHNHSAYAMIEKGIAH"
     gene            complement(5013..6011)
                     /gene="araD1"
                     /locus_tag="VN496_16015"
     CDS             complement(5013..6011)
                     /gene="araD1"
                     /locus_tag="VN496_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HXF18515.1"
                     /translation="MRLVQFTDRSGARRVAASEDGKTLRVLTGVTRIYDLALLAARTG
                     SSLETAAKAKVGAETQNYDEIVSEKRLLPPFDHPDPYHCLISGTGLSHLGSAAARDSM
                     HAKLQKADSELTDSMKMFKWGIDGGKPAKDKIGTAPEWFYKGDGRCVAAPEAELELPA
                     YALDGGEEVEIVGVYVIGDDGEVLRVGYALGNEYADHVMERQNYLYLAHSKLRQCSFG
                     PELLIGMLPESITGRARLLRDGKELWSEDWLSGEANMCHSIGNLEHHHFKYRDFRHPG
                     DVHVHFYGAATGSFTKKIETRVGDVFEIHSPQFGRPLRNPLGAAREADHLVTVRPL"
     gene            6161..6943
                     /gene="pcaD"
                     /locus_tag="VN496_16020"
     CDS             6161..6943
                     /gene="pcaD"
                     /locus_tag="VN496_16020"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HXF18516.1"
                     /translation="MKVKTNGIQLDYSVEGQGPWLVMSHSLACNQSMWDEQAQLLKSR
                     YRVLRFDTRGHGGSDAPAGAYTLEMLSEDLRGLLDSLSVEHPHFVGLSMGGMIGMTFA
                     LKYPKRFRTLVLCDTSSRIPPEAGPVWEGRIKTAAEHGMEPLVEPTLQRWFTEPFYKS
                     NKAMMTRVAQMIRTTNPAGYIGCCKAIPGINVTDRLSALTLPVQVIVGEQDVGTPVAM
                     SRAIQDAIAGSEIVVIPSASHLSNLEQPAAFSKAVTGFIDRH"
     gene            complement(6947..8353)
                     /locus_tag="VN496_16025"
     CDS             complement(6947..8353)
                     /locus_tag="VN496_16025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HXF18517.1"
                     /translation="MLDRSLIFLDVETTGATAHVDRITEIGLVEVDQGRHVGEWSSLV
                     NPERPIPLTVESLTGITDAMVANAPTFAELAGDLYDRLAGKTLVAHNARFDFGFLRNE
                     FRRVGIRYAPDVVCTVRLSRRLFPQERAHNLDSVIARHGLSCEHRHRALPDARVLWDF
                     ARRIHQDLDTAAIQKAVADQLQKPRLPPGLPVDLPDQLPETPGVYAFFAHDGTPLHVG
                     KSANLRARVLSHFSSDRQPAWRATNEIAQIEWDSAVGELGTQLRHTQWLKRLQPVHNS
                     KPRQDETLWSLHWDPIEGPSIPLPVDAGDVDLCRASSLHGMFRSRRAALNALRKVADE
                     HGLCHIATGLQTGPGPCLGHQLRRCRGTCIGLETPIAHSMRMIKALHALRMRDWPHPG
                     PIGVREHDAITGRTEVHVLDQWRHLGSAEEEDALQDILQSRTDVPFDASVYRILLQYF
                     KSPPRDMKIVALESSTAV"
     gene            8462..9010
                     /locus_tag="VN496_16030"
     CDS             8462..9010
                     /locus_tag="VN496_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18518.1"
                     /translation="MSSRHLPWLLLCTALQAFAHSESSVPTNGILDCQHPPENMAAAL
                     PKAMSSTATILCTPSAQMIVAQEGWAWRFPGSFFDRPSIPAYSPVESRPIAGGRYFID
                     FKVNELTSAEIKKLHDTFTKALVTYTATAPPVRIFKLVARNDQGHSLDAYFGFQSSNE
                     GWVALCAPGCEPEMFFLTNRHD"
     gene            9022..10206
                     /locus_tag="VN496_16035"
     CDS             9022..10206
                     /locus_tag="VN496_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXF18519.1"
                     /translation="MELIAEKVLPADAQRATLIGRAWVPGRNAGPSPVLVADGKVWDL
                     VRVVPTVSELLNLQDPVKVAKSAVMTGWARPVGSVASLINNSHADRRDAAKPYLLAPC
                     DLQALRACGVTFVSSMLERVIEEHAKGDQDKADEIRRSIGAEIGAEIKTIRPGSPEAK
                     RVKDSLIRRNLWSQYLEVGIGPDAEVFNKSQPMSAVGVGAEIGVRADSSWNNPEPEVV
                     LAINRAGQVVGAALGNDVNLRDFEGRSALLLGKAKDNNGSCAIGPFIRLFDRDYGIED
                     LRRTHIHIQIEGPDGYQLSGESHLANISRDPLDLASQAVNRTHQYPDGTMLFLGTQFA
                     PVQDRDQPGKGFTHRIGDVVSIRADALGMLVNRVNHCDKVVPWTFGINDLMRNLSGRG
                     LL"
     gene            complement(10292..11149)
                     /locus_tag="VN496_16040"
     CDS             complement(10292..11149)
                     /locus_tag="VN496_16040"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXF18520.1"
                     /translation="MPRWQQRRERLRSVLFGTQCVYPGSVFDPISARIAEELGFEIGM
                     FAGSIASLTVLGAPDLILLTLTEFAEQAHRICRAGNLPLLCDADHGYGNALNVMRTVE
                     ELETAGVAGLSIEDTELPAAFGGKQPRLISIEEGIGKMRAALAARRDTALVIAGRTSS
                     PVINGIADTIERARAYEAAGVDAIFLVGLKSRADLDAVAAQVKLPLILGGAGKELMDR
                     DWLAGRGVRICLQGHQPFTAAVRAIHDTLRALRDGVPPEAIGALASDETMKRLTGTAD
                     YARWMKDWL"
     gene            complement(11175..12101)
                     /locus_tag="VN496_16045"
     CDS             complement(11175..12101)
                     /locus_tag="VN496_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXF18521.1"
                     /translation="MKNPSKPGRRRLLQAVGGIAASTMVTRSFAEAKVLATRPIPSSA
                     EQLPVVGLGTYQSFGVGADVAEREPLQQVLRLFVEHGGKLVDSSPMYGSSEAVLGDLA
                     AATGVANRLFMATKVWTSGREAGIRQMEESFVRMKVTRMDLMQIHNLLDWKTHLPTLK
                     QWKQAGRIRYTGITHYHSGAYSELERLMKMREFDFVQFNYSIVEREAEERILPLARET
                     GTAVIVNRPFAQASLFSRVRGKQVPEWAAEFECTSWGQFFLKYILAHPAVTCAIPATA
                     KPEHLLDNMMAGVGKLPDESTRKKMALYIDNL"
     gene            complement(12010..13404)
                     /locus_tag="VN496_16050"
     CDS             complement(12010..13404)
                     /locus_tag="VN496_16050"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF18522.1"
                     /translation="MSLLADSPFLRRFVPVRREEIAATLWSFSYFFCLLCSYYLLRPV
                     RDEMGIQGGVENLAQLFTGTFVIMLASVPLFGWASSRFPRAKLLPIVYLFFIVNLAIF
                     YALMRSGVAPAIVARAFFIWTSVFNLFVVSVFWSFMADLFRNEQARRLYGLISAGGSA
                     GALAGPLLATVLAPSLGPASLLPISAAMLCAAVLCIYRLTAWAKRNPAERRFPPAQVE
                     EPLGGGIFAGITLALKSPYLLGICIYVMLGTLLGTFLYFHQANIMAVEMPSAGARTAL
                     FAKIDLAVNVLTFACQIFLVGRLMNRFGVGTTLMILPVMGAIGFFILGLMPTLAVLVI
                     FQIIRRAGEYAIARPAREVLFTVLNREEKYKSKNFIDTVVFRSGDAASGWLFEGLRIL
                     GLGFAGIAFVGVPVALLWAGTGWMLGRSQDEMRARNEAIRRPDEKSKQAGAAPATASG
                     GGNRRIDDGDPVVR"
     gene            13587..14798
                     /locus_tag="VN496_16055"
     CDS             13587..14798
                     /locus_tag="VN496_16055"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HXF18523.1"
                     /translation="MSITLQDIQNAALAIAGAVVDTPCLLSRTLSEAAGAQVYLKFEN
                     HQFTASFKERGALNKLLSLDSQQRRAGVIAVSAGNHAQGVAYHAKRLGIPAVIVMPRY
                     TPHVKVEQTRAFGAEVILHGGNFDEAKEQALQVMSQRRLTLVHPYDDAKIIAGQGTIA
                     PEMLRVQPELDVIIVPVGGGGLISGIASAAKAMKPGIEIIGVETLRFPSLYSALKGRK
                     PVFGTNSIADGIAVKEPGKLTLEMVRRLVDEIVLVDEGDIEHAMLLLLEIEKTVVEGA
                     GAAALAGLLCNRERFAARNVGVILSGGNIDPLTLADIIERGMVRAGRLARLQVELRDL
                     PGSLAKVTAALAEANANIEEVHHERAFTRLPVQSAVVNFVLQTRGHDHVQQIIAGLEA
                     AGFKASLHNAD"
     gene            complement(15042..15638)
                     /locus_tag="VN496_16060"
     CDS             complement(15042..15638)
                     /locus_tag="VN496_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXF18524.1"
                     /translation="MAKTIDYYFSPMSPWTYLGHARFTEIARRHDAAINPKPVDYGRI
                     FPVSGGLPVAKRPAQRQAYRLVELQRWHDYLGLPMNIQPKYFPYDSRLAALAIIAAGD
                     TDKALKLTAALLRGCWAEEKNMADERELAVAIRACGLDSGEVLERAMAPETASRFEAF
                     TNEAIERQVFGAPTYIYKQELFWGQDRLEFLDRALEKG"
     gene            15768..>16709
                     /locus_tag="VN496_16065"
     CDS             15768..>16709
                     /locus_tag="VN496_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXF18525.1"
                     /translation="MKNRLRVLAASAALLCACTSQAQEIILKVHHPLPPSSTAHQKVL
                     EPWCAKLAMESKNRIKCQIYPSMQLGGSVPQLYDQVKDGVVDVIWTVAGYSAGRFPLV
                     EVFELPFFMHNAESASKALWDYVQQYDADEFKDVHAIAFHLHGGGVFHMIKKPIVHRS
                     DLRGTKVRAPTRQTTKMLAALGATPVGMPVPQVPEALSKGVIDGTILPYEVMPALKVD
                     ELTKYHSGPDASQPMIYNSVFVMAMNKAKYDSLPPDLRKIIDANSGIALSGQIGRLFA
                     EVEVANKQKVGGIMNVISNQEIEEWKKVVQPVTDGWVA"
BASE COUNT         3644 a         4785 c         4746 g         3534 t
ORIGIN      
        1 gagccagatc gcgccttgtt tgggcatctc gccattgtta taacgcagaa cagcttcatg
       61 ccacttttga taagcctcag taccgggctt gccgagtgcc tggttgattc ccacggtttg
      121 cacgctgtac cagtcgccaa actcccattt aagatcgtcg gcattcacaa attgcccgag
      181 cccaggatga aaaggcgcca cgtgatagtc ttcgaggtaa acctcgataa atgttttcca
      241 attgaccgaa tattcgtggc gcacgattcg gtcaaggcgg taaccggaaa agtcgaggtc
      301 ggccaggaca ccgagccgcg caaggtcgag attgggatcg cgtggtcccg caaactgcag
      361 accattccag ccggttaacc cggtcttgcc gaggtcaagg caagggtttc ccggaaaatg
      421 cggtgcaccc aacaatttcc ccgacagatc gtaagtccac cggtgcaatg ggcatacgat
      481 gttatgcgtg tgtccactgc ctttgagcat gatcgattgt cggtggcggc aaacgttgga
      541 aagcaattcc accccgttct ggctgcgcac taaaactttt gcattgctca tccagtcaag
      601 cgagcggtag tttccgactt ccggaaccat gagttcgtgg ccgacgtagc cggggccgtt
      661 ttcgaacaga accttgcgct cgatttcggc aatctccgga tcaaaatacc attcgatcgg
      721 taactggacc ggcgttcggg tcaaggccgc aacattagcc agatctgtca taactagatg
      781 tccctgctgc gtaaactaca aaaaaaaacg ccccggcgcg atatagaaag tggggcggaa
      841 ttctgaacac gattttccct taaaacccta ccggacgcaa cgttttgccg gttgatcgcg
      901 catgtaacgt actaatcaga ctactgttcg ttgacccggg ttccggtgat gggctatttt
      961 gatggtcttt cgtaacgagc cttggcagcg tcacgtgaca aaatccaaat cccagtcacc
     1021 cttatccttt caggccccgc cgtccttcga agccgcactg gccgaaatag aaaatatcgt
     1081 tgccaggatg gaagcgggac agttgccact ggacgaatca ctcctggcat acaaacgcgg
     1141 agcagagttg cttcagtatt gccaggcccg acttcaggag gcacaacagc aggtcaaagt
     1201 gcttgaagcc ggcacactgc agaatctaga cggtgacgac agcgccgaat gagttccagg
     1261 gctggatgct cgatcgacag gcccgcatcg agacaacgct gcaatccgcg ctgccggcat
     1321 ccgagatcgc gcctgcgcgc ctgcattccg ccatgcgcta cgccgtattg ggagcaggca
     1381 agcgggtcag accattgctt gcttacggag ctggtgagct ggcttgtgcg caaatagaaa
     1441 gagtggacta cgcagcagct tcagtggaac tgatccacgc gtattcactc gtgcacgacg
     1501 acatgccttc aatggacaac gatatactgc gtcgtggaag gccgactgtt catgtggagt
     1561 tcgacgaagc aacggccctg ctggtcggcg acagtctgca gacactggca tttcagctct
     1621 tgtcggaacg tgccgtcgcg gatgatgcaa gccggcagct ggaaatgatc gcgctgctgg
     1681 cgagggccag cggttcgcgg ggcatggcag gcggacaggc aatagacctg gaatccactt
     1741 cgcaggccct ctcggtacca gagctggaat acatgcatat tcacaaaact ggcgcgctga
     1801 ttcgtgcatc ggttgcgctc ggcgctcgct gtggtggcgc gttgagcgat gcgcagctgc
     1861 atcaactcga tcatttcggc aaattcatcg gccttgcatt ccaggtcgtg gatgacgtgc
     1921 tcgacgctca gtccactacc gcgacgttag gcaaaaccgc cggcaaagat gcacgcaatc
     1981 aaaaacccac ctacgtcagc gcactgggta ttgagcgttc gcgcgcgctg gctggcgaac
     2041 tgcgcgataa cgctctgcaa gcgctggatt ggcttcctga cggaggcgga catctgcgcc
     2101 agcttgcgga ctttatcgtc ctcagaaaat tttgatcctg caccagccca actttcgtcc
     2161 gacccgactt cccttatgta tgagctgctg aataccatcc agtccccttc cgaattgcgc
     2221 gcgctagagc gaaagcaatt gcccgagctc gctcgccagt tgcgggaatt tctgctcgag
     2281 tcggtcgcga agacgggagg ccacctttct tcgaatctcg gcacggtgga actgaccatc
     2341 gcgctgcatt acgtgttcaa cacgcctcag gacagactgg tatgggatgt tggccatcag
     2401 acttacgcac acaaaatact caccggcagg cgggatgcga tgtccacctt gcgccagtac
     2461 ggcggcattg gagggtttcc gcggcgttcg gaaagtgagt ttgacacttt cggaaccgct
     2521 cattccagca cgtcaatcag cgcggcactt ggcatggcag tggctgcgca catgaaggga
     2581 gagaatcgcc gcgtggtggc catcattggt gatggcgcca tgaccgccgg catggcattc
     2641 gaggcgctga acaacgccgg ggcaatggac gcgaatcttc tcgtagtatt aaacgacaac
     2701 gatatgtcga tttcagaaaa cgtcggcgcg ctcaataact atcttgcccg tctgatatcc
     2761 ggccggttct atgccgcggc ctggaaaaca ggtgagaagg tattgcgggt tgtcccgcct
     2821 gttctgcaac tggcaaaacg cgccgaggag cacgtcaagg gcatgatgac tcccggtacc
     2881 ctgtttgaag aattcggctt taactacatt gggcccattg atggccacaa tctcgatgtt
     2941 ctggtaacga cgctcggcaa tatcaaaaag ctcgacggtc cccaatttct gcatgtggtc
     3001 acccgtaagg ggaagggcta ccaacaggcc gagggcgacc ccattctcta tcatggggtc
     3061 acgaagttcg agccgacgct cggcatcgcg ccgaagggtg cgtccaagcc gacttacacg
     3121 caggtgtttg gagactggct gtgcgatatg gctgctcgcg attcgcgact gatcggcatc
     3181 acgccggcaa tgcgggaagg ttccggcctg gtccgcttct cgcaggaata cccggacagg
     3241 tatttcgacg ttggcattgc cgagcagcac gccctgacat ttgccgcagg gctggcttgc
     3301 gaagggttca aaccagtcgt tgccatttat tcaacgtttc tgcaacgtgc ttacgatcag
     3361 ctgatccatg acatatgcat ccagaatctg ccggtattgt tcggcattga ccgagcgggc
     3421 ctcgtcggtg cagacgggcc gacgcacgct ggcagcttcg atctttccta tctgcgttgc
     3481 ctgccgaaca tggtgatcat ggtgccatct gatgaaaatg aatgccggca gatgctctac
     3541 accggattca agctggatgg gcctgttgcc gtgcggtatc cgcgcgggag tggcccaggc
     3601 gtggccattg aacctgagat gattgagctg ccggtgggca agggcgagat acgacgccgc
     3661 ggcagcaaac tcgccatcct ttgctttggc gccttgctca aaccgtgcat ggaggctggc
     3721 gaaaaactcg gcgccacagt ggccaacatg cgctttgtca aaccgctcga tgaggagctg
     3781 atccgggaac ttgcgcgcga ccacgagcat ctagtcaccg tggaagaaaa caccgttctc
     3841 ggtggggccg gcagcgcagt cctcgaggtt ttggaaaaac ggggagtgtc gcggcgaacg
     3901 ctgcagctgg gattgcccga tcgcttcatc gatcacggag atccggcaaa actgttgcag
     3961 gattgcggcc tcgatgccaa tggaatcttc cactcgattg ccgcatttat agctgactag
     4021 tttactagga tattggcggc gtttataatc tgcaacgtgc tgtcacttcg ctccagagga
     4081 gcaacgagat gaattctccc gacaacttca ccattcccga tgtgcagggc tccgcggaca
     4141 cgcgccgtct cgctatcgac aaagtgggca tcaaatcgat tcgacatccg gttcgcgtgg
     4201 gcgatcgcga cggcggcgtc cagcatacga ttgcgacgtt caacatgtac gtcggcctgc
     4261 cgcacaattt caagggcacg catatgtctc gatttgtcga gatactgaat gctcatgaac
     4321 gcgaaatatc cgtggagtca ttcgaagcca tgttgcgaga catggtcaaa cgtctcgaag
     4381 cggaattcgg gcgcatcgaa atgacattcc cgtatttcat caacaaggcg gctccagtgt
     4441 ccggcgtgca gagcctcatg gattacgaaa tcacgtttgc cggcgaacta tcgggtgcag
     4501 gctattcatt cacgatgaaa gcggtcgtgc cggtcacgag cttatgtcct tgttcaaagc
     4561 agatctcgga atacggtgcc cacaaccagc gatcacacgt gaccataacg gcgcgtacga
     4621 atagccatgt ctggatcgag gagttggtgc gtgttgccga gcaagaggct tcctgtgaac
     4681 tttacggcct gctcaagcga ccggacgaaa aatatgtcac ggaacgcgct tacgacaatc
     4741 caaagttcgt agaggatctc gtgcgtgacg ttgcaacggc gttgaatcgg gacagccgca
     4801 tcgacgccta tgtggtggaa tctgaaaatt tcgaatccat acacaatcat tctgcatacg
     4861 ccatgatcga aaagggcatt gcgcattagt attgcccctc gagaaaaaag cccgcatcgc
     4921 gggctttttt tacggcgttt ggtcgatgac ggtttttttt gttcatcgcc ccgcctttcc
     4981 cgcagcggcc gcaatccaaa cgatgacagc attcagagtg ggcggacggt taccaggtga
     5041 tcggcttccc gcgcggcgcc gagcgggttg cgcagcggcc gaccgaattg cgggctgtgg
     5101 atctcgaaaa catcgccgac cctggtttct atttttttgg taaagctgcc ggtcgcagct
     5161 ccgtaaaaat gcacatgcac gtcgcctgga tgacggaaat cgcgatactt gaaatggtga
     5221 tgctcgaggt tgccgatcga gtggcacatg ttggcttccc cggaaagcca gtcctccgac
     5281 cacagctcct tgccatcgcg cagcaggcgc gcacgcccgg tgatcgattc aggcagcatg
     5341 ccgataagaa gttcaggccc gaacgagcat tggcgcagtt tggaatgcgc cagataaaga
     5401 taattctggc gctccatgac gtggtccgcg tactcattgc ccagtgcata gccgacgcgc
     5461 aatacttcac cgtcgtctcc aatgacgtaa acgccgacaa tttcaacctc ttcaccaccg
     5521 tccagcgcgt aagccggcaa ttcgagctca gcttccggcg ctgccacgca acggccgtct
     5581 cccttgtaga accactctgg cgcggtgcca atcttgtcct tggccggttt gcccccatca
     5641 atgccccatt tgaacatttt cattgagtcg gtcagttcgg aatcggcctt ctgcaacttt
     5701 gcgtgcatcg agtcgcgtgc tgcggcactg ccaagatgcg ataacccggt tccgctgatg
     5761 aggcaatggt aaggatcggg atgatcgaac ggcggcagca accgcttctc gctcacgatt
     5821 tcatcatagt tctgcgtttc tgcgccgacc ttcgctttgg cagcagtttc taacgatgag
     5881 ccagtacggg cggccaggag cgcaaggtcg tagattcggg tcacgccggt cagcacgcgc
     5941 aacgtcttgc cgtcctcaga cgcggcaacc cggcgtgcac cggagcggtc ggtgaattga
     6001 acaagcctca aaatattctc ccccttttgg ttgatgcggt tttatgatcc cgcctggatg
     6061 ccggacactg gaatgttacg actaaacacg ccgacccggg tcgctgtcgt actgacggcc
     6121 gttgccaacc ctgtactgcg ccgttcgcac cggaggatcg atgaaagtaa aaaccaatgg
     6181 gatccaactc gactattccg ttgagggcca gggtccatgg cttgtcatga gtcattccct
     6241 ggcgtgcaac cagtcgatgt gggatgagca agctcagctg ttgaaatcgc gataccgcgt
     6301 gcttcgcttt gacacccgcg gacacggcgg cagcgatgcg cccgccggag cgtacacgct
     6361 cgaaatgctt tccgaggatc tgcgcggatt gctcgatagc ctgtccgtgg agcatccgca
     6421 tttcgtcggc ttgtccatgg ggggaatgat tggcatgaca ttcgcgctga agtatccgaa
     6481 gcgctttcgc acactggtct tatgcgatac gtcgagtcgt attccgcccg aagccggtcc
     6541 tgtgtgggaa ggccgcatca agactgctgc cgagcacggc atggagccgc tggtggaacc
     6601 gacattacag cgctggttta ccgagccgtt ctacaagtcg aacaaggcaa tgatgacgcg
     6661 cgtggcgcag atgatccgca cgacgaaccc tgccggttac attggttgct gcaaggcgat
     6721 cccgggcatc aacgtgaccg atcgcctttc ggcgctgacc ctgccggtgc aggtgatcgt
     6781 tggtgaacag gacgtgggta cgccagtggc catgtcgcgc gcaattcaag acgctattgc
     6841 tggatcagaa attgtcgtta tcccgtcggc atcgcatttg tccaatctgg agcagcccgc
     6901 cgctttcagc aaagccgtga ccgggttcat cgaccggcat taatagttat acggcggtcg
     6961 atgattccag cgcgacgatt ttcatatcgc gcggaggcga tttgaaatac tgcagaagta
     7021 ttctgtaaac acttgcatca aatggcacgt cggtacgcga ttgcagaatg tcctgcaatg
     7081 cgtcttcttc ttcagccgag ccaagatgcc gccactgatc cagcacatgc acctcggttc
     7141 tgcctgtaat ggcgtcgtgc tctcgcacac cgatcgggcc aggatggggc cagtcgcgca
     7201 tgcgcaatgc gtgcagggcc tttatcatgc gcatcgaatg cgcgatcggg gtctccagtc
     7261 cgatgcaggt tccgcggcag cgcctgagct ggtggccaag gcacggcccc gggccagtct
     7321 gcagtccggt ggcgatatgg cataaaccat gttcatcggc gactttgcgc aatgcattca
     7381 gcgcggcgcg tcgcgatcga aacatgccgt gcaaactcga agcgcggcat aaatccacgt
     7441 cccccgcatc gaccggcaac gggatcgacg gtccttcgat cggatcccag tgcaaagacc
     7501 atagcgtttc atcctgcctg ggcttgctgt tatgcacagg ctgcagcctt ttcaaccatt
     7561 gtgtgtgccg cagctgcgtg ccgagctcgc caacagcgct gtcccattcg atttgcgcaa
     7621 tttcgttggt agctcgccat gcgggctgcc tgtcgctgct gaaatgagac aaaacccggg
     7681 cgcgtagatt tgcgctcttg ccgacatgca gaggcgtacc gtcatgcgca aagaatgcat
     7741 acacgccggg cgtttccggt aactggtcgg gtagatcgac aggcagtccc ggcggcaatc
     7801 gcggtttctg caactgatct gcaacggcct tctgaattgc cgccgtatcg agatcctgat
     7861 ggattcgccg cgcgaaatcc cacaagacac gcgcgtccgg cagcgctcga tgccgatgct
     7921 cgcaggacag gccgtgacgc gcgatcacgc tgtccagatt gtgcgcgcgt tcctgtggaa
     7981 acaggcgccg ggacagccgc acggtgcaca ccacatcggg agcgtatctt atgccgacac
     8041 gccggaattc gttgcgaaga aaaccaaaat caaagcgcgc gttatgcgcg acaagagttt
     8101 taccggccag gcgatcataa agatctcctg cgagctcggc gaaggttggt gcgttggcca
     8161 ccatggcgtc agtgatgccg gtcagggatt ccaccgttag aggaatcggc cgttccgggt
     8221 taaccagcga actccattcg ccgacgtgcc ggccctgatc gacctcgacc agaccgatct
     8281 cggtgatccg gtccacgtgc gcggtggctc cagtggtctc cacatcaagg aagatcagcg
     8341 aacggtcaag catgagcgct gcggatttgt ttcagcatgc gttaatttta ccttccctgc
     8401 ccagggccgg tacatgcgcc gccgactgta taatccgcca caaataacat cgggagaatt
     8461 catgtcatcg cgccatctgc cttggctgtt gctttgtact gcgctgcagg cgtttgctca
     8521 ttctgaatcc agcgtcccga caaatggcat tctcgactgc cagcatcctc ctgagaacat
     8581 ggcagccgcg ctgccaaaag caatgtcatc gacggccacc attctgtgca cgccttccgc
     8641 acaaatgatc gtcgcgcaag aaggatgggc ttggcgattt ccgggcagct ttttcgatcg
     8701 gccttccatt cctgcttact cgccggttga gtcacggccc attgccggcg gccgttactt
     8761 cattgacttc aaggtcaatg aactgacgag tgctgaaata aaaaaacttc acgacacttt
     8821 cacaaaagcg cttgtcacgt atacggctac agcaccgccg gtacgcattt tcaaactcgt
     8881 ggcgcgcaac gaccagggcc attctctgga cgcgtatttc ggatttcaat ccagcaacga
     8941 agggtgggtc gcgctttgcg cgccgggttg cgagccggaa atgttttttc tgaccaaccg
     9001 ccacgactga ccggagaccg catggaactc attgccgaga aagttctgcc cgccgatgcg
     9061 caacgcgcta cgctgatagg acgcgcctgg gtgcctggac gcaatgccgg accaagtccc
     9121 gtgctggttg cggacggcaa ggtgtgggac cttgtgcgcg tcgtgcccac ggtcagcgaa
     9181 ttgctcaatc tgcaagatcc ggtcaaggtt gcaaaaagcg ccgtgatgac cggttgggct
     9241 cgacccgttg ggtccgtcgc gagcctgatc aataattcgc atgcagaccg ccgcgacgcc
     9301 gccaagcctt atctccttgc accatgcgat ttgcaggcac tgcgagcatg tggcgtgacg
     9361 ttcgtgtcca gcatgctgga acgggtcatc gaggagcatg ccaaaggcga tcaggataag
     9421 gctgatgaaa tcaggcgctc catcggcgcg gagatcggcg ccgaaatcaa aacgatcagg
     9481 ccaggctccc ctgaagcgaa gcgcgtcaag gattcgctta tccggcgcaa cctttggtcg
     9541 caatatctgg aagttggcat cggaccagat gccgaggtgt tcaataagtc ccaaccgatg
     9601 tcggcggtcg gtgtgggcgc ggaaatcggc gtgcgtgctg attcatcctg gaataatcct
     9661 gagccagaag tagttctggc aatcaatcgc gccggccagg tggttggcgc ggcgctgggt
     9721 aatgatgtca atttgcgcga tttcgaaggg cgaagcgcgt tgctgttggg caaagccaag
     9781 gacaataacg gcagttgtgc catcggcccg ttcattcgcc ttttcgaccg cgactatgga
     9841 attgaagacc tgcgccgcac gcatattcac atccagatcg agggcccgga cggctatcag
     9901 ctttccggcg aaagccatct tgctaacatc agccgcgatc cgcttgacct tgccagccag
     9961 gcggtcaacc gtacgcatca gtatccagac ggcaccatgc tttttctcgg gacgcaattt
    10021 gcaccggtac aggatagaga ccaacccggc aaagggttca cacacaggat cggagacgtg
    10081 gtttcaatcc gggctgatgc cctcggcatg ctggtgaacc gggtgaatca ttgtgacaag
    10141 gttgtgccgt ggaccttcgg catcaatgat ttgatgcgaa acctttctgg ccggggattg
    10201 ttgtaaatac catcgaacgg tgtcattttg atcaaagcga aataaaccgt cccttgctgc
    10261 taaaacaatt cactaggcca ggcagtcctc gttaaagcca gtccttcatc cagcgagcat
    10321 aatcggcggt tcccgtcagc cgtttcattg tttcgtcgct tgccagtgcg cctatggctt
    10381 ccggcggcac gccgtcccgt agcgcgcgca gcgtgtcatg gattgcgcgt acggcggcgg
    10441 taaacggctg atgtccttgc aggcagattc gcacaccgcg gcccgccaac caatcacgat
    10501 ccataagttc cttgccggca ccgcccagga taagcggtag ctttacttgt gcagcgaccg
    10561 catccaggtc ggcgcgcgat ttcagtccga ccaggaagat cgcatcgacg ccggccgctt
    10621 cataggctcg cgcacgttcg atggtatccg caatgccgtt gattacagga gaactggtgc
    10681 gccccgcaat caccaatgct gtatcccgtc gagccgccag cgcagcgcgc atcttgccaa
    10741 ttccttcttc gattgaaata agacgcggct gcttgccgcc gaaagcagcc ggcagttcgg
    10801 tatcctctat ggacaagcct gccacacccg ccgtttcgag ttcctcgacg gtgcgcatga
    10861 cattcagtgc attgccatag ccatggtcgg cgtcgcacag cagcgggagg tttccggcac
    10921 ggcagatgcg atgtgcctgt tccgcgaact cagtcagcgt gagcagtatg aggtccggcg
    10981 ccccgagtac ggtaagggaa gcgatggatc ccgcaaacat gccaatctca aagcccagtt
    11041 cttcagcaat tcgcgcggag atcggatcga aaaccgatcc aggataaacg cactgagtgc
    11101 cgaaaagcac ggagcgcaaa cgctcgcggc gttgctgcca gcgtggcatg gatgattcct
    11161 gtctgcgcaa tgcgctacaa gttatcgatg taaagggcca tttttttgcg cgtgctttcg
    11221 tcaggcaact ttcccacgcc cgccatcata ttgtccaaaa gatgctcggg cttggccgtg
    11281 gcggggatcg cgcaagtcac ggctggatgt gcaaggatgt atttcagaaa aaattgtccc
    11341 catgacgtgc actcgaactc ggctgcccat tcgggcacct gtttaccgcg cacgcgtgag
    11401 aacagactcg cttgtgcgaa tggacggttt acgatcacgg ccgtgccggt ttcgcgggca
    11461 agcggcagaa tgcgctcctc ggcctctcgc tccacgattg aataattgaa ctgcacgaaa
    11521 tcgaattccc gcatcttcat caagcgttct agttcggaat aagcgccgga atggtaatgg
    11581 gtgataccgg tgtagcgaat gcgaccggcc tgtttccact gcttcagtgt aggcagatgg
    11641 gtcttccagt cgagcagatt gtggatctgc atgagatcca tgcgcgtcac cttcatgcgc
    11701 acaaaagatt cttccatctg gcggatgccg gcttcgcggc cgctggtcca gaccttcgtg
    11761 gccatgaaca gcctgttggc aacgccagtt gccgccgcaa gatcacccag cacggcttcc
    11821 gaagagccat acattggcga actgtcaacc aatttgccgc cgtgctcgac gaatagccgc
    11881 agcacctgct gcaacggttc ccgctccgcc acatccgcac ccacaccgaa actctggtaa
    11941 gtgccgagtc cgaccaccgg caattgctct gcgctgctcg gaattggacg ggtagcgagc
    12001 accttggctt cagcgaacga ccgggtcacc atcgtcgatg cggcgattcc ccccaccgct
    12061 tgcagtagcc ggcgccgccc cggcttgctt ggatttttca tcaggtctcc tgatagcctc
    12121 gttccgcgca cgcatttcgt cctggcttcg tccgagcatc catcccgtgc cggcccagag
    12181 cagcgcgacc ggcacgccga caaatgcaat gccggcaaat ccgagcccaa gaattctcag
    12241 cccttcgaac agccatccgc tcgctgcatc accgctgcga aaaaccacag tatcgatgaa
    12301 gttcttggat ttatatttct cctcgcggtt caacaccgtg aacaggactt cgcgcgccgg
    12361 gcgggcaatc gcatattccc ccgcccggcg aatgatctgg aatataacca gcaccgcaag
    12421 agtcggcatc agaccaagaa tgaaaaatcc aattgcgccc atcacgggaa gaatcatcag
    12481 cgtggtgccg accccgaagc gattcatcag gcgccccacc aggaaaattt ggcacgcaaa
    12541 ggtcagcaca ttgaccgcaa ggtcgatctt tgcaaacagt gcggtgcgcg cgcccgctga
    12601 tggcatttca acggccatga tattcgcctg gtgaaaatac aggaacgttc ccagcagcgt
    12661 tccgagcatc acgtagatgc aaatgccaag caaatagggc gatttcagtg ccagcgtaat
    12721 tcccgcaaag ataccgccgc cgagaggttc ttcgacctgg gccggcggga agcggcgctc
    12781 agcgggattt cgctttgccc atgcggtgag ccgatagata caaagcacgg cggcacacag
    12841 catggccgcg gaaattggca gcaggctggc cgggccgagc gatggcgcca gcaccgttgc
    12901 gagcagcgga ccggccaacg ctccggcact tccgcccgcg gatatcaatc cataaagcct
    12961 gcgagcttgt tcgttgcgga acaaatccgc catgaagctc cagaacaccg atacgacaaa
    13021 aagattgaac acgctggtcc agataaaaaa tgcccgggcc acgatcgccg gcgcaacgcc
    13081 ggaccgcatc agtgcgtaga agatggcgag attgacaatg aagaacagat acacgatcgg
    13141 taatagcttg gcgcgtggaa agcgcgaaga agcccatccg aacaagggca ccgaggccaa
    13201 catgatgacg aaagtgccgg tgaacagttg tgcgaggttt tccacgccgc cctgtatgcc
    13261 catctcgtcg cggaccggtc tgagcaagta ataactgcat agcaggcaga agaaatagct
    13321 gaatgaccaa agcgtagccg cgatttcttc tcgtcgaacg ggcacgaatc ggcgcaagaa
    13381 gggactgtcg gcgagtaaac tcattggcag gcattgtagt caacccgccg gtatcaggca
    13441 ccttcaatgt gcagtttcag agacggccat ggcgcagcct tttttgcaag cccgcccttt
    13501 gatcggacgc ctgaagtgcg atggccaatt tgcgtgctcc ggatcacgtg acttagagtg
    13561 cgccttattc gaacccggca cttgccatgt caattactct tcaggatatt cagaatgcgg
    13621 cgctcgccat cgcaggtgcg gtggtcgata ccccatgcct gctgtcgcgt acgctttcag
    13681 aggcggccgg cgcacaggtt tatctcaaat tcgagaatca tcaattcacc gcatcattca
    13741 aggagcgcgg tgcgctgaac aagctgctct cccttgactc tcaacagcgc cgtgccggcg
    13801 tcattgcggt ctcggctggc aatcatgcgc agggcgtggc ataccatgca aaacgcctcg
    13861 gtatcccggc tgtcatcgtc atgccgcgct acacgccgca cgtgaaagtg gagcaaacgc
    13921 gtgctttcgg agcagaagtc atcctgcacg gaggaaattt cgacgaggcc aaggagcagg
    13981 cgttgcaggt aatgtcgcag cgccggctga ctctggtgca tccctatgac gatgcaaaaa
    14041 tcattgcagg gcagggaacc attgccccgg aaatgctgcg tgtccaaccg gagctcgatg
    14101 tcattatcgt cccagtcggt ggaggtggct tgatctctgg tattgcatcc gccgccaagg
    14161 caatgaagcc cggaatagag atcattggcg tggagacctt gcgctttccg tcgctgtatt
    14221 cggcgttgaa aggacgcaaa cccgtgttcg gcaccaactc gattgccgac gggatcgcgg
    14281 tgaaggaacc cggcaagctg acactggaaa tggtacgccg gttggtggac gagatcgttc
    14341 ttgtggatga gggcgatatt gaacacgcca tgctgctgct gctggaaatt gaaaagaccg
    14401 tggtggaagg cgccggcgca gcagcacttg cggggttact gtgcaaccgc gaacgattcg
    14461 ccgccagaaa cgtcggggta atcctgtccg gcggcaacat cgatcctctg acacttgccg
    14521 acatcataga gcgtggcatg gtgcgcgcgg gtcgccttgc gcggctgcaa gtcgaattgc
    14581 gcgatctgcc cggctcgctt gcgaaagtca ctgcggcgct agccgaagcc aatgccaaca
    14641 tcgaagaggt gcaccatgag cgtgccttca cgcgtctgcc agtacaatcc gccgtagtga
    14701 attttgtgct gcagacgcgc gggcatgatc atgttcagca gattattgcc ggactggaag
    14761 cagcgggttt caaggcttcg cttcataacg cggactgagc agaaaaacag gactgaggtt
    14821 cgaggactga ggactgtgaa aaaaacgtga cggtcatgac gacgcctaaa ggccgtaccc
    14881 caaacgcggg attccctata agaccgccct gactcttagt acgcagtcct gccttttcct
    14941 tgactcagtc cgcagttccg tttttacgtc acgtcgtcac gtcgtcacgg atcttttgac
    15001 tttttactca gtcctcagtc ctcatcgctc agtcctatct tctatccttt ctcgagcgcg
    15061 cgatccagga attccaaccg gtcctgtccc cagaataatt cctgcttgta aatatacgtt
    15121 ggcgcaccga atacctggcg ctcgatcgct tcattggtga aggcctcaaa acgggaagct
    15181 gtttcgggcg ccatggcgcg ctcgagcact tctccgctgt ccagcccgca cgcccggatt
    15241 gccacggcaa gttctctctc atcggccatg tttttttctt cggcccagca tccgcgaagc
    15301 aaggccgccg tcaacttcaa tgctttgtcc gtgtcgcccg ctgcaataat cgccagcgca
    15361 gcaagccggc tgtcgtaagg aaaatacttc ggttgaatat tcatcggcaa gcccagatag
    15421 tcgtgccagc gctggagttc gaccagccgg taggcttgcc gctgcgccgg ccgcttggca
    15481 acgggcaagc cgccggacac tgggaaaatc cggccatagt cgaccggttt tggattgatc
    15541 gcggcatcgt gcctgcgcgc gatttccgta aaacgtgcgt gccccagata ggtccacgga
    15601 gacatcggcg aaaagtagta atcgatggtc tttgccacgc gcaactcctc ttgctatggt
    15661 gttggtgttt ggatgaagca gggcgcaagt ttatcatcgg gattcacagc ataattcggg
    15721 tgcgcatcca tcatggccgg tgtgcgaatt actgaacgga ggaaacagtg aagaatcggt
    15781 tgcgtgttct cgcggcttcg gcggcactgt tgtgcgcctg cacgagtcag gcgcaggaaa
    15841 taatattgaa agtccatcat ccgctgccac cgtcctcgac agcgcatcag aaagtgctcg
    15901 agccatggtg cgcaaagctc gcgatggagt cgaagaaccg cattaaatgc cagatctatc
    15961 cctcaatgca gttgggcggg tccgtgccgc aactctacga ccaggtaaag gacggcgtgg
    16021 tggacgtcat ctggactgta gcgggctatt ctgctggccg ttttccgctg gttgaagttt
    16081 tcgaattgcc gtttttcatg cataacgccg aatctgccag caaggcttta tgggactacg
    16141 tgcagcagta tgatgcggat gaattcaagg acgtacacgc cattgcattt cacctgcacg
    16201 gcggtggcgt gtttcacatg atcaagaaac cgatcgtgca tcgatcggat ttgcgcggga
    16261 caaaagtgcg cgccccgacg cgccagacga ccaagatgct ggccgcgctc ggcgccacgc
    16321 ccgttggcat gccggtgccg caggtgccgg aagccctgtc gaaaggcgtt atcgacggca
    16381 ccatacttcc gtatgaagtg atgccggcgc tgaaggttga tgaactcacc aaataccaca
    16441 gtggcccgga tgcctcgcag ccgatgatct acaactctgt attcgtcatg gcgatgaaca
    16501 aggccaagta cgattccttg cctccggatc tgagaaaaat catagatgcc aacagcggca
    16561 tcgcactgtc cggccaaatc ggacgtctgt tcgctgaagt agaggttgcc aacaagcaaa
    16621 aggttggcgg catcatgaac gtgatttcca atcaggaaat cgaggaatgg aagaaagtcg
    16681 tgcagccagt gacagacggc tgggtggcc
//
LOCUS       DATMZQ010000505         4012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1019243, whole genome shotgun sequence.
ACCESSION   DATMZQ010000505 DATMZQ010000000
VERSION     DATMZQ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019243"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VN496_16070"
     CDS             <1..968
                     /locus_tag="VN496_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXF18526.1"
                     /translation="QVIKIAEGQELHVFGWLASEQGNLESPLVEQLVQLKAQLEQASD
                     VAIVFGAEVSGKAIEQLVAFGSKLPGRTRYMALGDYSNSRGAADMGLLPDRLPGYAYV
                     DNATARDAFAQLWGGVLPTKAGMTAPQMVEAAQAGKLRALYVVGANPLTHYGTLGFGR
                     GNLQLLIAHELFPTDTSRPADIVFPAASAYEKDGTVTNTSGEIQLLRKAAEVMGARSD
                     FDLLRIVSHQLEKVGIGKAFHYKNPAAVFEEIRKAVAGYDVQPAGLLTGGAEPTRVHV
                     ARNGHAPYDVPAGLIRSAQDTLFTSGTLGRYCTMMDSLQEADIQP"
     gene            965..2191
                     /locus_tag="VN496_16075"
     CDS             965..2191
                     /locus_tag="VN496_16075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXF18527.1"
                     /translation="MIDFLTNFVSGHAEIIITLVKIALLLFIVLTVNAYLTWFERKVV
                     AHMQSRWGPHRVGPHGLLQPLADGAKFLLKEDPTPAGVDKFTYFLAPLMALALALTSI
                     AIIPFGPDPLRVFGHDIYLGIVDLNIGILALFAITALSVYGVALAGWSSNSKYPLLGG
                     LRSSAQMVSYELALTMSVVGVLLMAGSFSLRDIVNAQASHPELGIFGWNIWPQILGFL
                     CFFIAAIAETNRVPFDLPEAESELVAGFHTEYSSFKFAMFFIAEYTSMITVSCLCSIM
                     FFGGWLSIFPASWTFTHYLPSLILIPFGLWVIYDGIRYETIFGRVILPGLGTVITVLG
                     ALFIALPAVNEFIQGPFWLLSKIFVFLFVYVWMRGTLPRFRYDQLMAFGWKLLLPVSL
                     ANVVITATAILFWGKH"
     gene            2191..2694
                     /locus_tag="VN496_16080"
     CDS             2191..2694
                     /locus_tag="VN496_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXF18528.1"
                     /translation="MNAPLIVFFVLAIMAVLGAISLIVQKHPIHSALSLIVVMVALAG
                     LYLLMGAEFVAAVQIIVYGGAIMVLFVLVIMLLNAGVEERTSFSKLATLAGLPLTIAL
                     LGFMAATVARSSAIVQDAAQPGAAAPTKQIAGLLFQQFVYPFELTSFLILVAILGAIV
                     LAQREKA"
     gene            2696..2998
                     /gene="nuoK"
                     /locus_tag="VN496_16085"
     CDS             2696..2998
                     /gene="nuoK"
                     /locus_tag="VN496_16085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXF18529.1"
                     /translation="MVPTHYYLILSAILFGLGTVAFVFRRNIITIFMSIELMLNAVNL
                     AFVAFSQAWNRLDGQVFVLFVIVVAAAEAAVGLGIIILIARVRQSLNVERVDLLKL"
     gene            2998..>4012
                     /locus_tag="VN496_16090"
     CDS             2998..>4012
                     /locus_tag="VN496_16090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXF18530.1"
                     /translation="MPILDNLWIIPLLPLLGSAINGLFGKKWPNKIVNSVAVGSTGLA
                     FLCALEAVREFFQLSPEQIPWVKQYFSWIVAGNFRAGFDLQIDQLTVVMLLVVTGVGW
                     LIHIYSTGYMAHEGGYYRFFSYLNLFMFFMLILVLAANYVLLFVGWEGVGLCSYLLIG
                     FYFLKKSASDAGKKAFIVNRIGDFGFMLGMFLLFRTFGTLDFVPLFAKASPMPADLMG
                     QYGTLTIACLLLFMGATGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTAGVYVCAR
                     SHVLFTHSPSAMLIVAIIGCATALFAATIGLVQTDIKRVLAYSTVSQLGYMFLAVGVG
                     AFSA"
BASE COUNT          665 a         1253 c         1149 g          945 t
ORIGIN      
        1 cgcaggtcat caagatcgcc gaagggcagg aacttcacgt tttcggctgg ctggccagcg
       61 aacaggggaa tctcgagagc cccttggttg agcaactggt gcagctcaag gcgcagctcg
      121 aacaggcttc cgatgtggcc attgtgttcg gcgcggaagt ttccggcaag gccatcgagc
      181 agctcgtggc cttcgggtcg aagctgcccg gcagaacgcg ctacatggcg ctgggcgatt
      241 acagcaactc gcgcggtgcc gccgacatgg gactgctgcc cgatcggctg cccggctacg
      301 cgtatgtcga caacgccacc gcacgcgacg cgtttgcaca attgtggggc ggcgtgctgc
      361 ccaccaaggc gggcatgacc gcgccgcaaa tggtggaggc cgcgcaggcc ggcaagctgc
      421 gcgcgcttta cgtcgtgggc gccaatcccc tgacacacta cggcactttg ggcttcggcc
      481 gcggcaatct gcaattgctg atcgcgcacg agctattccc taccgatact tcgcggcccg
      541 cggacatcgt atttccggcg gcatcggcgt acgagaaaga cggcacggtc acgaatacat
      601 ccggcgaaat tcaattgctg cgcaaagccg cggaagtcat gggcgcgcgc agcgactttg
      661 atctgctgcg cattgtttcg catcagctgg agaaagtcgg gatcggcaag gctttccatt
      721 acaagaatcc cgccgcggtg ttcgaagaga tccgcaaagc cgttgctggc tacgatgtgc
      781 aacccgcggg cctgttgacc ggcggcgccg agccgacgcg cgtgcatgtg gcgcgcaacg
      841 gccacgctcc gtacgacgtt cccgccgggc tgattcgctc cgcgcaggac acactattca
      901 cgagcggcac gctggggcgc tattgcacca tgatggactc tctgcaggag gccgatatcc
      961 agccgtgatc gacttcctta ccaactttgt gtcggggcac gctgaaatca tcatcacgct
     1021 cgtcaaaata gcgctgttgc tcttcatcgt gctaacggtg aacgcgtacc tcacctggtt
     1081 tgagcgcaag gtcgtggcgc acatgcagtc gcgctggggg ccgcaccgcg tcgggccgca
     1141 cgggttgctg caacctctgg ccgatggcgc taaatttttg ctgaaagaag atccaacacc
     1201 tgcgggcgtg gacaaattca cgtacttcct agcgccgctg atggcgctgg ccttggcgct
     1261 gacttccatc gccatcattc cttttggtcc cgacccgctg cgcgtcttcg gccacgacat
     1321 ctatctcggc atcgttgatc tcaacattgg catcctggcg ctgtttgcca tcaccgcgct
     1381 gagcgtttac ggcgtggcgc tggccggctg gtcttcgaac agcaaatacc ctctgctggg
     1441 cgggctgcgc agctccgcac agatggttag ctatgaactg gcgctcacca tgtccgtggt
     1501 cggcgtgctg ctgatggcgg gcagcttcag cttgcgtgac atcgttaacg cccaggcatc
     1561 ccatccagaa ctaggcatct tcggctggaa catttggccg cagattctcg gcttcctgtg
     1621 cttcttcatc gccgccatcg cggaaacgaa ccgcgtgccc ttcgatcttc ctgaagccga
     1681 atccgaattg gtcgcaggct ttcacaccga atattccagc ttcaagtttg ccatgttctt
     1741 cattgctgaa tacactagta tgatcactgt ctcttgcctc tgctcgatca tgttcttcgg
     1801 cggctggctc tccatcttcc ccgcctcgtg gactttcacg cactatctgc cttcattgat
     1861 cctgattcct ttcggtctat gggtgattta cgacggcatc cgctatgaaa cgatctttgg
     1921 gcgcgtcatt ctcccgggac tcggcacggt aattaccgtg ctgggcgcgc tgtttatcgc
     1981 gctgcccgcg gtgaacgaat ttatccaggg cccgttctgg ctgctctcca agattttcgt
     2041 cttcctgttc gtttacgtgt ggatgcgcgg cacgcttccg cgcttccgct atgaccagct
     2101 gatggctttc ggctggaagc tgctgttgcc ggtatcgctc gccaacgtgg tgatcaccgc
     2161 aaccgccatt ctgttctggg ggaagcacta gtgaacgcac ccctcatcgt tttcttcgtg
     2221 ctcgcgatca tggccgtgct cggcgccatc agcctgatcg tgcagaagca tccgattcac
     2281 agcgcgttgt cgctgatcgt tgtgatggtc gctctcgctg gcctttacct gctgatgggc
     2341 gccgaatttg tcgccgcggt gcagatcatc gtttacggcg gcgcgatcat ggtgctgttc
     2401 gtcttggtga tcatgctgct gaacgcgggg gtcgaggagc gcaccagctt ttccaagctc
     2461 gccacgctgg ccggattgcc gctaacgatc gccctgctcg gcttcatggc tgccaccgtg
     2521 gcccgttcca gcgccattgt gcaagacgcc gcgcaacccg gcgcggccgc gcctaccaag
     2581 caaatcgcgg gcctactctt ccagcaattt gtgtatccct ttgagctgac gtcattcctg
     2641 attctcgtcg cgatcctcgg cgccatcgta ctggcgcagc gggagaaagc ctgacatggt
     2701 cccgacgcac tactacttga tcctcagcgc catcctgttt ggcctgggca cggtcgcctt
     2761 cgtatttcgc cgcaatatca tcaccatctt catgtccatt gagctgatgc tcaacgccgt
     2821 gaacctcgcg ttcgtggctt tcagtcaggc gtggaaccgt ctcgacggac aggttttcgt
     2881 tcttttcgta atcgtggtcg cggccgcgga agcggctgtc ggactcggaa tcatcattct
     2941 cattgcgcgc gtacggcagt cgctcaacgt cgagcgcgtg gatttgctca aactttaatg
     3001 ccgatactcg acaatctctg gatcattccg ctgttgccgc tgctcggctc ggctatcaac
     3061 ggcctgttcg ggaaaaagtg gccgaacaag atcgtcaatt ccgtggccgt cggctcgacc
     3121 ggcctagcgt tcctgtgcgc gctcgaagcc gtccgcgaat ttttccagct ctcgcctgag
     3181 cagattccct gggtgaagca gtatttctcc tggatcgtgg ccggaaactt ccgcgcaggt
     3241 ttcgatctgc aaatcgatca gctcaccgtc gtgatgctgt tggtggtgac cggagtcggc
     3301 tggctcatcc acatctattc caccggctac atggcgcatg aaggcggcta ctaccgcttc
     3361 ttctcctatc tcaacctgtt catgttcttc atgctcatcc tggtcctggc cgcgaattac
     3421 gtgctgttgt ttgtcggatg ggaaggcgtg ggcctgtgca gttacctgtt gatcggattc
     3481 tacttcctga aaaagtccgc gagcgacgcg ggcaagaaag cgttcatcgt caaccgcatc
     3541 ggtgatttcg gcttcatgct cggcatgttc ctgctgttcc gcacctttgg cacgctcgat
     3601 ttcgtgccgc tgttcgcgaa ggcgtcgccg atgccggcgg atctgatggg gcagtacggc
     3661 acgctcacga tagcctgcct gctgctgttt atgggcgcca ccggcaagtc cgcgcagctc
     3721 cccttatatg tctggctgcc cgacgccatg gaaggcccga cccccgtttc cgccctcatc
     3781 cacgcggcca ccatggtcac tgcgggcgtt tacgtttgtg cgcgctcgca cgttttattc
     3841 acgcactcgc cgtcggcgat gctgatcgtc gcgatcatcg gctgcgccac cgcgctgttc
     3901 gccgccacca tcggcctggt gcagaccgac atcaagcgcg tgctcgccta ttccacggtc
     3961 agtcagcttg gctatatgtt tctggcggtg ggcgtcggag cgttttccgc cg
//
LOCUS       DATMZQ010000506         4897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5078665, whole genome shotgun sequence.
ACCESSION   DATMZQ010000506 DATMZQ010000000
VERSION     DATMZQ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5078665"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VN496_16095"
     CDS             <1..237
                     /locus_tag="VN496_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HXF18531.1"
                     /translation="HADWPGLLQAISETGAARVIATHGYANVLARWLQQRGLQTSTFE
                     TEFGDEETGMPPGINDEPVGETRSAENVSEELAR"
     gene            234..1874
                     /locus_tag="VN496_16100"
     CDS             234..1874
                     /locus_tag="VN496_16100"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXF18532.1"
                     /translation="MKRFAQLYAELDTTTSTREKLAALARYYAKAPAADAAWATYFLA
                     GGKPRQVVPTRLLREIALRASGLDDWLLDECYLAVGDFSETVALVLPPPKLQDETGLA
                     EWVEHRLAPLRGAAPELVERRLRDYWDSLGTRERFLLGKLISGAFRVGVSRLLVQRAL
                     AAHAGIDTKTVAQRMMGYTDGSVSPTAENFAALLDAAAAHRGGGQPYPFFLAHQLNAA
                     PSTLGDVHDWMIEWKYDGIRAQVVHHGGKVWIWSRGEDLVTTRFPEIEAVGRALPEGT
                     VIDGEILVWQDERPRPFALLQQRIGRKTLTNKILAQAPVALIAYDLLEQAGSDIRALP
                     QRERRLRLERMIAAARQARPELPLRISPMITGLSWDALGILRAESRTRGVEGFMLKHQ
                     AASYGVGRTKDVGTWWKWKIDPYSVDCVLVYAQRGNGRRASLYTDYTFAVWNGPELVP
                     FAKAYSGLSDEEFRGVDAIIRRTTVEKFGPVRSVTPTLVFELGFEGIQRSSRHKSGVA
                     VRFPRMLRWRKDKNIAEANSLADLQELLPKVGDFAHET"
     gene            1864..2790
                     /locus_tag="VN496_16105"
     CDS             1864..2790
                     /locus_tag="VN496_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18533.1"
                     /translation="MKPEIIFFKVRIRSWQTLFFCALVAPGLFAPCALAAVLNTDEAA
                     GLHTRYRQLHSRLQSNAFQKPLYLESNEGDGEIQADVYAVLDYPEHTVVSELKQPAHW
                     CDILILHPNTKYCRPASQDTPTVLNVNIGKKLDQALEQSYRVDFEYHLIAASPDYLAV
                     YLNAERGPFGTQNYRFLLEAVPLNDKQTFMHLSYSYAYGTAARLALMAYLKTSGSHKV
                     GFTVVDRDNDGQPVYIRGVRGLMERNTMRYQLAVETYFGALSVPPDARPEKRLMDYYA
                     ASERYPRQLHDVEQQDYLTLKRKELTRQQSAR"
     gene            complement(2822..3451)
                     /locus_tag="VN496_16110"
     CDS             complement(2822..3451)
                     /locus_tag="VN496_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXF18534.1"
                     /translation="MKPADWYFDFISPFAYIAFTRLERLSDGLQLRYHPVLFAGLLNH
                     WEQKGPAEIPGKRIWTYRWCTWWAAQQGIAFRLPAVHPFNPLPYLRLAIAAGNTPQVI
                     QRIFEALWTTGTDPGDPHQFAALARSLEVDASLLADQAIKDSLKRETEQAIACGVFGV
                     PTFIVDGELFWGADAMEFLEAYLANPELLASNEMKRVGTLPVGAARKNP"
     gene            complement(3545..3979)
                     /gene="trxC"
                     /locus_tag="VN496_16115"
     CDS             complement(3545..3979)
                     /gene="trxC"
                     /locus_tag="VN496_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HXF18535.1"
                     /translation="MSDSLHIVCPNCSSTNRVPAERTGAGPRCGKCHQALFSGHPVEL
                     DEAAFERHVASNDVPVVVDFWAPWCGPCRAMAPEFEKAAAMLEPAARLVKINTEVAQG
                     LAQRFNIRSIPTMALFRGGQEIARQSGAMGATAIASWVRANV"
     gene            complement(4077..>4897)
                     /locus_tag="VN496_16120"
     CDS             complement(4077..>4897)
                     /locus_tag="VN496_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXF18536.1"
                     /translation="ASIASEIEAIERNLAILKPPSSLDAWEAHHRGLWHMYRFDKTEN
                     ERARQFFETAVNLDPTFARAYAGLSFTHFQNAFQGWKKREPEIERAYAAAGQSVMVDD
                     RDPAAHWAMGRALWLRDRHAQSVIELEQAIDLSPNFALAHYTLAFIHSQAGDPDTAIS
                     ASDFSRHLSPFDPLLFGMLGARAMALVRLERFEEAAGWAVKAAARPNAHAHIMAIAAY
                     SLALAGALPEAHAHAAAIRKMIPQYGLADFLGAFRFDALGAALFRKGAQLIGMA"
BASE COUNT         1056 a         1356 c         1470 g         1015 t
ORIGIN      
        1 cacgccgact ggcctggact gctgcaagcc atatcggaaa ccggagcggc gcgcgtcatc
       61 gccacgcatg gatacgcgaa tgtgctggcg agatggttgc agcagcgcgg attgcaaacg
      121 tctacgttcg agactgagtt cggcgacgaa gaaaccggca tgccgcccgg cataaatgat
      181 gaaccggtcg gcgagactcg ttcagcggag aacgtcagtg aggaactggc gcgatgaaac
      241 gctttgccca gctgtacgcc gaactcgata ccacgacctc gactcgggaa aaacttgcgg
      301 cgctggcgcg ctattacgca aaggcgccgg cagccgacgc ggcgtgggca acgtatttcc
      361 tggcgggcgg gaagccgcgc caggtggtgc cgacccgcct gctgcgggag atcgctttac
      421 gtgcgagcgg actcgacgac tggttgctgg acgaatgcta tcttgcagtc ggcgatttct
      481 ccgagaccgt tgcgctcgtg ctgccaccgc ccaagttgca ggatgagacc gggctcgcgg
      541 aatgggtgga gcatcgtctg gcgccgctgc gcggcgcggc acccgaactg gtcgaacgtc
      601 ggctgcgcga ctactgggac agtcttggaa cacgggaacg tttcctgttg ggcaagctga
      661 tttccggggc gtttcgtgtt ggcgtctcac gtttgctggt gcagcgggcg cttgcagcgc
      721 atgcgggcat agataccaag accgtggcac agcgcatgat gggttacacc gacgggagtg
      781 tgtcgcccac agcggaaaat tttgccgcat tgctcgatgc agcggcggca catcggggcg
      841 gtggccagcc atatccgttt ttcctggcac accagctcaa tgcggcgccc tccaccttag
      901 gcgatgtgca tgactggatg attgaatgga aatacgacgg cattcgcgcg caagtggtgc
      961 accacggcgg aaaggtatgg atctggtcgc gcggggaaga tctcgtgaca acgcgatttc
     1021 cggaaatcga ggccgtgggg cgtgccctcc ccgaggggac ggttatcgat ggggaaatcc
     1081 tggtttggca ggacgagcgt ccgcggccgt tcgcgctgtt gcagcagcgt atcggtcgca
     1141 agacgctcac caacaagatt ctggcgcaag cacctgtggc attgatagcc tatgatttgc
     1201 tcgagcaggc agggtccgat attcgtgcgc tgcctcagcg cgaacggcgt ctccgcctgg
     1261 agcgcatgat cgcagccgcg cgacaggccc gtccggaatt gccgcttcgc atttcgccga
     1321 tgatcacggg cctttcttgg gacgcactcg gcattctgcg tgcagaaagt cgcacgcgag
     1381 gcgtggaagg tttcatgctg aagcatcagg cggcatcgta tggcgtcggc cgcaccaagg
     1441 atgtcggtac ttggtggaag tggaaaatcg atccatacag cgtcgattgc gtgctggtgt
     1501 atgcgcagcg cggcaacgga cgaagggcca gcctgtatac cgattacacg tttgcggtgt
     1561 ggaatggccc ggaattggtg ccgttcgcca aggcttattc cggattgagc gacgaagaat
     1621 ttcgcggagt ggatgccatt atccggcgta ccactgttga aaagttcggt ccggttcgca
     1681 gcgtaacgcc aacgcttgtt ttcgaactgg gattcgaggg catccagcgt agcagccgtc
     1741 ataaatcggg cgtggcagtg cgtttcccgc gcatgctgcg ttggcgaaag gacaagaata
     1801 tcgctgaagc gaattccctg gctgacctac aagaacttct gcccaaggta ggagatttcg
     1861 cgcatgaaac ctgagattat ttttttcaaa gtcaggatca gatcctggca gacgctattt
     1921 ttctgcgcac ttgttgcgcc aggattgttt gcgccttgcg cgctggcagc agtattgaac
     1981 acggatgagg cggccggact tcataccaga tacagacagc tgcattcccg gcttcagagc
     2041 aatgcgttcc agaagccgct atacctcgaa tcgaatgaag gggacggcga aatacaggcc
     2101 gatgtgtatg ccgtgctgga ctatccagag catacagtgg tgtccgaact gaaacagccc
     2161 gctcactggt gcgacatcct tatcctgcac ccgaacacaa aatattgccg gcctgccagc
     2221 caggacacgc cgacagtgct gaacgtcaac atcggcaaga agctggatca ggcgctggag
     2281 caaagctatc gcgtggattt cgaatatcat ctgattgcag ccagccctga ttatctcgcc
     2341 gtatatctga atgccgaaag agggccattc ggcacgcaaa actatcgttt tctgctcgaa
     2401 gctgtgccgc tgaacgacaa gcagactttc atgcatctgt cctactcata tgcttatggc
     2461 acagctgcaa ggctcgcgct tatggcttat ctcaaaactt ctggcagcca taaagttggt
     2521 ttcaccgtgg ttgaccggga caatgacgga caaccggttt atatccgcgg cgttcgcggt
     2581 ctcatggagc gcaacaccat gcgctatcaa ttggcagttg aaacttattt tggtgctttg
     2641 tcggtaccgc ctgacgctcg accggaaaaa cggttgatgg actactatgc cgccagcgag
     2701 cgttatcccc ggcagctgca tgatgtcgaa cagcaggact accttacttt gaagcgcaag
     2761 gaactaacgc gccagcaatc cgcgcgataa aaagtttcat gcgacggggc tgcgcgagct
     2821 ctcaaggatt tttgcgcgcg gccccaactg gcaatgtacc gacgcgcttc atttcgttgc
     2881 tcgccaggag ctcaggattg gcaagataag cttcaaggaa ttccattgcg tccgctcccc
     2941 agaataattc gccatcaacg ataaacgtcg gcacgccaaa caccccgcag gcaatggcct
     3001 gctcagtttc ccgtttgagc gaatctttaa tcgcctgatc cgccaacagc gaggcatcca
     3061 cctctaatga ccgggcgagt gcagcgaatt ggtgcggatc gccaggatcg gtgcccgttg
     3121 tccagagcgc ttcaaaaatg cgctgaatga cttgcggcgt attgccggcc gcgatggcga
     3181 gccgaagata cggcaggggg ttgaacggat ggacagcagg caagcgaaaa gcaatgccct
     3241 gttgggcggc ccaccaggta caccagcggt aagtccagat gcgcttgccg ggaatttcgg
     3301 ccggtccttt ttgctcccaa tggttgagca ggcctgcaaa caacaccggg tggtagcgca
     3361 actgaagccc gtcggagagt cgttccagac gcgtgaaagc gatatatgca aacggtgaaa
     3421 tgaaatcgaa gtaccagtcg gccggtttca tacgcctttc agtttccaag caagcatcgc
     3481 tataccgctg gcattatgca gtctggtgcg tgtgcgcagc gaaaacgctt attgacttga
     3541 acgctcaaac gttggccctt acccagcttg caatcgctgt tgcacccatt gcacctgact
     3601 gacgggcgat ctcttgtcca ccgcgaaaca gtgccatggt cgggatgctg cgtatattga
     3661 aacgctgtgc cagaccttgc gcgacctcgg tgttgatttt taccagtcga gcggctggtt
     3721 ccagcattgc ggccgctttt tcgaattcgg gcgccatggc gcggcaaggt ccacaccaag
     3781 gggcccaaaa atccactaca acgggcacgt cgttgcttgc tacgtggcgc tcgaaagcgg
     3841 cttcgtcaag ctctaccggg tgtccgctga acagcgcctg atggcacttg ccacaacggg
     3901 gcccggctcc cgtccgctcc gcgggcacac gattggtact gctgcaattc gggcatacga
     3961 tatggagtga gtcactcata agcggtcctt ccgatactga tgatgtatcg gatattgttg
     4021 ccgccgaggc aaataaaaag ggttcaagca aacattcttt tgatggaaaa agagcatcac
     4081 gccatgccaa tgagctgtgc acccttgcgg aacagggctg caccaagcgc atcgaacctg
     4141 aaagcgccca ggaaatcggc aaggccgtac tgcggaatca ttttgcgaat ggcagcggca
     4201 tgtgcatgcg cctcggggag cgctccagcc agcgccaggc tataggcggc aatggccatg
     4261 atgtgtgcat gcgcatttgg tctggctgct gccttgactg cccaaccggc cgcctcctcg
     4321 aatcgctcaa gacgaacaag ggccatggcg cgcgcgccaa gcatgccaaa cagtaacgga
     4381 tcgaacgggc tcaggtgacg ggaaaaatcg gatgccgaga ttgcggtgtc aggatcgccc
     4441 gcctgggaat gaataaacgc aagtgtgtag tgcgcgagcg cgaaattcgg actcagatcg
     4501 atggcttgtt ccaattcgat gaccgactgt gcatggcgat cgcgcagcca aagcgcccgg
     4561 cccatggccc agtgcgcggc gggatcccga tcgtcgacca tgacactttg tccagccgct
     4621 gcgtatgcgc gctctatctc gggctcgcgt ttcttccaac cctgaaaagc attctgaaaa
     4681 tgcgtgaagg ataggccggc ataggcacgc gcgaacgtgg gatcgaggtt taccgccgtt
     4741 tcgaaaaact gccgtgcccg ttcgttttcg gtcttgtcga accggtacat gtgccatagc
     4801 ccgcgatgat gggcttccca tgcatcaagc gagcttggcg gtttcagtat tgcgaggttg
     4861 cgctcgattg cttcgatctc gctggcaatg gatgcga
//
LOCUS       DATMZQ010000507        13416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5078986, whole genome shotgun sequence.
ACCESSION   DATMZQ010000507 DATMZQ010000000
VERSION     DATMZQ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5078986"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VN496_16125"
     CDS             <1..862
                     /locus_tag="VN496_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXF18537.1"
                     /translation="TRLDKIRGVTEETTTGVHRLYQMHKDGKLAFPAINVNDSVTKSK
                     FDNLYGCRESLVDGIKRATDVMIAGKVAVVCGYGDVGKGSAQALRALSAQVWVTEVDP
                     ICALQAAMEGYRVVTMDYAADKADIFVSATGNFHVITHEHMKRMKDQAIVCNIGHFDN
                     EIDIASLAQYRWEEIKPQVDHVIFPDNKRIIILAKGRLVNLGCGTGHPSYVMSSSFAN
                     QTLAQIELFANTAKYPVGVYTLPKHLDEKVARLQLKKLNAQLTQLTDTQATYIGVDKG
                     GPYKADHYRY"
     gene            888..1733
                     /gene="metF"
                     /locus_tag="VN496_16130"
     CDS             888..1733
                     /gene="metF"
                     /locus_tag="VN496_16130"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HXF18538.1"
                     /translation="MTVELSAVRTFSFELFPPKTEDGKAKLRTTWQELARLKPRFFSV
                     TFGAGGSTQQGTLDTVLDIRAAGEQAAPHISCVAASKADILETLSNYREHGIRHIVAL
                     RGDMPSGLASGGELRYANELVDFIRQTTGDWFHIDVACYPEYHPQTRNPSDELKNFKL
                     KVDAGADSAITQYFYNPDAYFRFVDECAAAGINIPIVPGIMPIGNFSQLARFSDACGA
                     EIPRWIRIKLEGYRDDNSSIRAFGIDVVTALCEKLLAGGAPGLHFYTLNQAGSASIIW
                     QRLGL"
     gene            1821..3638
                     /locus_tag="VN496_16135"
     CDS             1821..3638
                     /locus_tag="VN496_16135"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXF18539.1"
                     /translation="MPRLNANPLVLLLATTAIAVFCELFWLNWLQTTEFRFTDLFISG
                     QARNIKPDPDIIVVAADDQSLQQLADYAGRWPWPRSIHAELVQGIEAQRPRAIVFDIM
                     FFEPDIFRLDADELFNKSVARYSNVYFPTVRKDPAGDAYGLPLVAMQAAIGAFAGPSA
                     DKNAMLNIGLPKALSPDNWRLGTIDFVADRDGIARRYFVYQDAYGWKIPSLPTRVLQD
                     LGLSVPDVESILLSWPGGKTGRPHVPYADVYTDFNSQNRRRDPNEFKDKIVVIGVTAT
                     GLHDMRPAPVAALYDGVDVLAAALDNLKNKNYLRDIQPAWPLAIALGLLVLLFAGFAA
                     RLHTLKIGGALILLSGFLVVVQHVALGKLVVIPVLRPLLFAWAFFFITALHVFLRERQ
                     VVREWSRLVSPKAVNELIAHGGWSRDGESRQVTVLFSNIRGLMSHSESRSPQEVVSMV
                     NRYFTRQVEVIFRNGGTLDKATGDCMMAIWGAPLDDARNAEHAIQCALEMADMLDVFR
                     KELDADADFDLGIGIHSGPAVVGLIGSEQRREYAAIGDTVILGSQIAGLTNGIARILV
                     SEQAMRLCPDAFSFVPRGLYKIKGTSPELALYEPNRKTA"
     gene            3670..4365
                     /locus_tag="VN496_16140"
     CDS             3670..4365
                     /locus_tag="VN496_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HXF18540.1"
                     /translation="MFGHTLSERERPALLAGASGLVGGYVLRRLLAHPSYARVHVLVR
                     QELPLSHPKLVQHIVDFENLQADLAAGVQDVFCCLGTTIRQAGSQQAFRHVDHDYPIA
                     LATLGKAAGAKQFLLVSALGADAHSRIFYNRTKGETERDILDIGLSRVLYMRPSLLLG
                     DRSRPRLGETVGAMVGGLIGPLFIGSMRKYRPIAADDVAAAMLYAATHDIGSGAIESN
                     EIAQLAVRENTQT"
     gene            complement(4401..5642)
                     /locus_tag="VN496_16145"
     CDS             complement(4401..5642)
                     /locus_tag="VN496_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXF18541.1"
                     /translation="MSTQDYAPKRKINSFYPPQRHLMGPGPSEIHPRVFSAMGKPTIG
                     YLDPVFVEMMEELKELLRYGFQTNNALTFPVSGPGSVGMEMCFVNMVTPGDKVIVCRN
                     GVFGGRMIENVQRCGGIAVVVEDRWGTPVSAQKVEDAFKANPDARILAFVHAETSTGV
                     ASDAKTLAEIARKYGALTIMDAVTSLGGTPVLVDEWGIDAVYSASQKCLSCTPGLSPI
                     SFNERVVEQVRNRKDKVHSWFMDMNLLLGYWGATNRTYHHTAPTNALYALHEALLMLR
                     EETLENAWARHQRNHLALKAGLETLGLNYLVEEAYRLPQMNAVSVANGIDEKEVRRRL
                     LLDYNLEIGAGLGDLAGKIWRFGLMGYSCKMENVMLSLCALETVFTDMGVNVRFGEAE
                     AAAYHAYAAHPSRLQPVRAVA"
     gene            complement(5853..6740)
                     /gene="ttcA"
                     /locus_tag="VN496_16150"
     CDS             complement(5853..6740)
                     /gene="ttcA"
                     /locus_tag="VN496_16150"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HXF18542.1"
                     /translation="MTTVQRQVYEGNKLAKRLRRLVGEAVGHFDMIQNGDKVMVCLSG
                     GKDSYAMLDVLLSLKNHAPIDFEIIAVNLDQKHPGYPAAVLPDYLTSIGVPFRIVEQD
                     THSVVKRVIPEGKTMCSLCSRLRRGALYRVARELGANKIALGHHRDDILETFFLNLFH
                     GGRLKAMPPKLISDDGQHIVIRPLAYCKEADLEEYARLRQFPIIPCNLCGSQQNLQRQ
                     VVKNMLQEWERKFPGRLETMFASLQRTSPSHLLDPRQFDFANLKTQPEAYKDGDIAFD
                     SEDFSARLIQFPAAKSANE"
     gene            complement(6741..7157)
                     /locus_tag="VN496_16155"
     CDS             complement(6741..7157)
                     /locus_tag="VN496_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HXF18543.1"
                     /translation="MSDNLDQRRDLTGEASAMPPCPTRRIFLKNFETRLSIGIHDFEK
                     RAKQRVVVNVDLYLDPDGKIERDHIDETVDYDFVRREIVGLADSSHFNLQETFCERIL
                     AICLARPGVRAARVSSQKLDVYKDCESVGFEIFRTR"
     gene            complement(7154..8146)
                     /locus_tag="VN496_16160"
     CDS             complement(7154..8146)
                     /locus_tag="VN496_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18544.1"
                     /translation="MKRAKLIQPPDAAISLRMLSLSRSLWRRASEEGSGKSMGREAAG
                     KVKCETVGLQFPFNNSCAVRRNLKRTMARAKKHQRVALVTGAGKRIGRAISLALAADG
                     WDIALHFHRGEAEAERLSAEISALGAKAVAVRCDLSDAEQVPELVEASERHLGTVTCL
                     VNNAAVFAYDDLASLETAQWHRHLDVNLFAPLLLSKTFAARLPDELTGCIVNMLDQKV
                     FNLNPDFLSYTVAKIGLEGATRLLALALAPKVRVCGVAPGITLVSGEQTAEGFARSHR
                     MAPLGRSSTVEDIADAVRFVVNAGALTGTTIVVDGGQHLWPSRRDVQFAVGNDQ"
     gene            8145..9182
                     /locus_tag="VN496_16165"
     CDS             8145..9182
                     /locus_tag="VN496_16165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXF18545.1"
                     /translation="MEMALYAPGQGYYVAGTAKLGEAGDFVTAPEISALFGRTLAHQV
                     AQVLEFSGADAVLELGAGSGTMAADVLGELRNIDRLPRRYVILETSPQLVQRQRDTLW
                     RRQPALNDRIQWVTALPENLDGVVIANEVLDALPVHLVAWRRDGLHERGVSWKDSFVW
                     EERLLQPGALRSAAEAIGAAPDYLSEISLLAPGLVRSLSNTVRRGALLLIDYGFGWRE
                     YYHPQRSRGTLMCHYRHLAHDDPFFLPGLQDVTAHVDFTAIAQAGMESGMDLLGYTTQ
                     AQFLINSGIIGWMEQQPERSSAARIAHTAGVQKLLSPAEMGELFKVIALSRGIEGPLM
                     GFTSGDKSRLL"
     gene            9298..10641
                     /gene="mpl"
                     /locus_tag="VN496_16170"
     CDS             9298..10641
                     /gene="mpl"
                     /locus_tag="VN496_16170"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HXF18546.1"
                     /translation="MHIHILGICGTFMGGIAVIARQAGHKVTGCDANVYPPMSTQLQA
                     QGIELIEGFDAGQIDLKPDLFVIGNVVSRGNPLMEQILDRGLAFTSGPQWLYENILRG
                     KWVLAVAGTHGKTTTTSMLAWILEHAGLNPGFLIGGVPLNFGISARTTDSSFFVIEAD
                     EYDTAFFDKRSKFVHYLPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTLPGSGLII
                     AHGREQSMRRVLAKGVWTKVEWLGIEQGWTARPRDAHAFDVFFEGGPRGTVNWELLGD
                     HNRNNALAALAASRHAGVPVAKGIDALAEFRNVKRRLEVSGAVNGVTVYDDFAHHPTA
                     IETTIAGLRSKVGNARILAVLEPRSNTMKMGIMKDALAGSLAGADLVFCYTNNLGWDA
                     QPVLASLGAKAQCFEDLGALVAAIVAHSQAGDYVLTMSNGAFGGIHDRLLEALAK"
     gene            complement(10690..11997)
                     /locus_tag="VN496_16175"
     CDS             complement(10690..11997)
                     /locus_tag="VN496_16175"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXF18547.1"
                     /translation="MTDDSRRRFFLQSGAVASAPWLGDVLKAEEQQRRPVPPGAMLGD
                     IHVLPATPHTTQWGWFDNAEPPVLTVRSGARISMETMMASANQVLPGVSIEAITQMRL
                     AFPGRGPHTITGPIYIEDAEPGDTLKVHIERILPRSYGANWNLPGELGLGQFPDRFPA
                     GQVRYFYFDQQTASTQFAPGVRIPLRPFPGVLGVARAEAGRYSTVPPGPFGGNMDVRE
                     MIEGTTVYLPVFVEGALLWSGDSHAAQGNGEINLTAIETAFNELRLRVDVLKGKSLEW
                     PRIETPTHWITVGYDRDLNKALEILKRETSTFLAESQDIHPEKTAEWMASRYDCRISE
                     VVNQMKGVFCMIPKSGVKLKRAVRPLRETPHYFVTSASSPDLQSAMNQAAFAMIEQIA
                     ERKNMSVIDAYSLMSLGMDSRLGDIGEFNKTVHCLVPKSLWTA"
     gene            12126..12695
                     /locus_tag="VN496_16180"
     CDS             12126..12695
                     /locus_tag="VN496_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HXF18548.1"
                     /translation="MIIYIHGFNSSPSSHKANQLRERLTSLGRAHEFFCPALPHWPAQ
                     AMALLEGLVSATPPADVTLVGSSLGGFYATCLTEKYGARSVLVNPAITPHEGLRSYLG
                     QQKNLYSGDEYLLTEEHLTQLARLHVAQPASMDRYLLMHTTGDELLDWRIAVDHYRGC
                     RQVIVKGSDHGFSEFAQYIDIVLSFADRS"
     gene            12807..>13416
                     /locus_tag="VN496_16185"
     CDS             12807..>13416
                     /locus_tag="VN496_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXF18549.1"
                     /translation="MNVFFEEDGAFKAGTVLADNHTSLQVENQHGKRTKVKEGAVLIR
                     FDHIPLSQFMAEAQSIAKDIDPAFLWECSGATEFGFDQLGKEYFGHEPRPAESAGLLM
                     RLHATPTHFYKKGKGRYKAAPADALRAALASVERKRKQADLQQRYAEQLARFEVPEEF
                     KPLLPQLLYRPDKGCIEYKALEQAAALAHLTIPRLLERSGAFA"
BASE COUNT         2806 a         3830 c         3944 g         2836 t
ORIGIN      
        1 cacgcgcctg gacaagatca ggggcgtcac cgaagagaca acgactggcg tgcaccgcct
       61 gtatcagatg cacaaggacg gcaagctggc cttcccggcc attaacgtga acgactccgt
      121 cacgaaatcc aaattcgata atctgtatgg ctgccgtgag tctctggtgg acggcatcaa
      181 gcgtgccacc gacgtgatga ttgccggcaa agtcgcggtc gtgtgcggct atggtgacgt
      241 tggtaagggc tccgcacagg ccttacgggc gctctcggca caggtctggg tgaccgaggt
      301 cgacccgatc tgcgcgctgc aggccgcgat ggaaggttat cgcgtagtca ccatggacta
      361 tgcggcggac aaagccgaca tattcgtcag cgcgaccgga aattttcatg tgataacgca
      421 cgagcacatg aagagaatga aagaccaggc aatcgtgtgc aacatcggcc actttgacaa
      481 cgaaatcgac atcgcctcac ttgcccagta ccgatgggag gagatcaagc ctcaggtcga
      541 ccacgtcatc ttcccggaca acaagcgcat catcatcctt gcgaagggcc ggctggtgaa
      601 cctcggttgc ggcaccgggc acccgagtta cgtgatgagt tcatcatttg cgaaccagac
      661 gctggcccag atcgagctgt tcgccaacac ggccaagtat ccggttggcg tttatacgct
      721 gcccaagcat ctggacgaaa aggtggcgcg cctgcagctc aaaaaactga acgcgcaatt
      781 gacgcaactc acggatacgc aggccaccta catcggagtg gataagggcg ggccttacaa
      841 ggcggatcat taccgatact agctgcctgc gggcgccccg ttagcacatg accgtcgagt
      901 tatccgcagt gcgtacgttc agttttgaat tgtttccgcc gaagacggag gatggcaagg
      961 cgaagttgcg cacaacatgg caggagcttg cgcggctgaa gccgcgtttt ttttccgtca
     1021 cgtttggcgc aggcggatcc acacagcagg gaacgctgga taccgtactg gacatccgcg
     1081 ccgccggtga gcaggccgca ccgcatatct cgtgcgtggc tgccagcaaa gccgacattc
     1141 tggaaacact gtccaactac cgtgagcacg gcatccgcca tattgttgct ctgcgcggcg
     1201 acatgccctc cggactcgca tccggcggcg aactgagata tgccaatgag ctggtggact
     1261 tcattcgaca gacgaccggg gactggttcc acatcgatgt cgcctgctat ccggaatacc
     1321 atccgcaaac gcggaatcct tccgatgagc tgaagaactt caagctcaaa gtcgacgccg
     1381 gtgcggattc tgccattacg caatatttct acaatccgga cgcgtatttt cgctttgtgg
     1441 atgaatgcgc ggcagcgggg atcaacatcc cgatcgtgcc gggtatcatg ccaatcggca
     1501 atttttcaca gctcgcaagg ttctcggatg cttgcggcgc agagatccca cgctggatcc
     1561 gcatcaagct ggagggctat cgggacgata actcgtccat acgcgcgttt ggaatcgatg
     1621 tggtaactgc gctgtgcgaa aaattgcttg ccggcggcgc gccgggcttg cacttttata
     1681 cgctgaacca ggcgggttca gccagcataa tctggcaaag gttgggcctg tagcagactg
     1741 caatcaggac gacacagaac acctgctgcg aagctttctg cctttcacct ttccacgcgt
     1801 ttcttttgta gactgcgcgc gtgccgcgcc taaatgcgaa tccgcttgtc ctgttgctgg
     1861 caacgaccgc cattgcagtc ttttgtgaat tgttctggct gaactggctg caaaccactg
     1921 agtttcgctt taccgatctt tttatttcgg ggcaggcgcg caacatcaag cccgacccgg
     1981 acatcattgt tgtcgccgcc gacgaccaaa gccttcagca acttgcagac tatgcggggc
     2041 gctggccttg gccacgctcc atccacgcgg aattggtgca aggcatcgag gcgcaaaggc
     2101 cgcgtgccat cgtgttcgac atcatgttct tcgagcccga tatctttcgg ctggacgccg
     2161 acgagctatt caacaagtcg gtagcgcgct acagcaacgt atattttccg acggtgcgca
     2221 aagaccccgc cggagatgcg tacggcctac cgctggtggc gatgcaggct gcaatcggcg
     2281 cattcgccgg cccttccgcg gacaaaaacg ccatgctcaa cataggattg cccaaggcgc
     2341 tgagtccgga caactggcgc ctgggcacga tcgattttgt cgctgatcgg gacggcatcg
     2401 cccggcgcta tttcgtgtat caggatgcgt acggctggaa aattccgtcg ttgcccacga
     2461 gagtgctaca ggacctggga ctgtccgtgc ccgatgtgga atccattctg ctgagctggc
     2521 ccggtggtaa gaccggtcgc ccgcatgtgc cgtatgcgga tgtatacacc gatttcaaca
     2581 gtcagaaccg aaggcgcgac ccgaacgaat tcaaggacaa aattgtcgtg atcggtgtca
     2641 cagccacggg actgcatgac atgcgaccgg cgccggtcgc cgcgttgtac gacggcgtgg
     2701 atgtgctggc cgcggcactg gacaacctga agaataaaaa ttacctgcgt gacatccagc
     2761 cagcgtggcc actagcgatc gcgcttgggt tgctggtttt gctgtttgcc ggtttcgctg
     2821 cgcggctgca tacgctgaaa atcggtggcg cgctgatcct gttgtcgggc tttctggtag
     2881 tagtacagca tgtcgcgctc ggcaagctcg ttgtcatccc ggtgttgcgt ccgctgctgt
     2941 tcgcgtgggc tttctttttt attactgccc ttcatgtatt tctgcgcgaa cgacaggtgg
     3001 ttagggagtg gtctcgcttg gtcagcccaa aagcagtcaa cgaattgatc gcacacggag
     3061 ggtggtcccg cgacggcgaa agccgccagg ttacggtgct gttctcgaac atccgcggat
     3121 taatgagcca ttcagaatcg cgctcgccgc aggaagtggt cagcatggtc aatcgctatt
     3181 tcacccggca ggttgaagtc attttccgca atggcggaac gctcgacaag gccacgggtg
     3241 attgcatgat ggcaatctgg ggtgcgccgc tcgatgatgc aaggaatgcc gagcacgcaa
     3301 ttcaatgtgc ccttgaaatg gccgacatgc tggatgtttt caggaaggag ctagacgccg
     3361 atgcggattt tgacctgggc ataggcattc attcaggacc ggccgtagtc gggctcatcg
     3421 ggtccgaaca gcggcgagaa tacgctgcga ttggcgatac agtgattctt gggagccaga
     3481 tcgcgggatt gacgaacggg attgcccgga ttctggtttc ggagcaagcg atgcgccttt
     3541 gtcctgacgc ttttagtttt gttccgcgcg gactctataa aatcaaagga acaagcccgg
     3601 agctcgcgct gtacgaacca aacagaaaga cagcgtgacg gcagcagcca cgaaggagac
     3661 gcgtgctccg tgtttggaca cacgctaagt gaacgcgaac ggccagcact gttggctggg
     3721 gccagcggcc tggtgggtgg gtatgtcctg aggcggttgc tcgcacatcc atcttacgcg
     3781 cgcgtgcacg tgctggtgcg ccaggaactg ccactgagtc acccgaaact tgttcagcac
     3841 attgtcgact ttgagaatct gcaagctgac ctggcggcgg gagtgcagga cgtcttctgt
     3901 tgcctcggca ctacgatcag gcaagcggga tcgcagcaag cgttcaggca cgtagatcat
     3961 gattatccga ttgcactggc gacacttggc aaggcggcag gtgcgaaaca atttctgttg
     4021 gtatccgccc ttggggccga tgcgcactca aggatcttct acaaccggac caagggcgaa
     4081 acggagcgtg atattttgga catcggtcta tcccgggtgc tctacatgcg gccgtcgtta
     4141 ctgctcggag accgctcgcg acctcgcctg ggcgagacgg tgggtgcaat ggttggcgga
     4201 ttgatcggtc cgctgttcat cggaagcatg cgcaagtacc ggccgattgc tgcggatgat
     4261 gtagccgcag ccatgctata cgcagcaact catgatattg gaagcggtgc gatcgagtcg
     4321 aacgagatcg cgcagctcgc tgtccgggaa aacacccaga cctgatcagg ccgggccttt
     4381 atttcccctt gcgaagcgac tcaggcaacc gcgcgcacag gctgtaagcg agagggatgg
     4441 gccgcgtagg catggtaagc cgcggcttcc gcttcgccga acctgacgtt cacgcccatg
     4501 tccgtaaaaa cagtttcgag cgcacacaag ctcaacatca cgttttccat cttgcaggaa
     4561 taacccatca ggccgaagcg ccagatcttg ccggccagat cgcccaggcc ggcaccaatc
     4621 tccaggttgt aatccagcag caggcgccgg cggacttcct tctcgtcaat accgtttgcg
     4681 acgctgaccg cgttcatctg cggcaggcgg taggcttctt ccaccaggta attcaaaccc
     4741 agcgtttcca ggccggcttt cagggccaag tgattgcgct ggtggcgcgc ccaggcattc
     4801 tcgagagtct cctcccgcag catcagcaaa gcttcatgca aagcatagag ggcgttcgtc
     4861 ggtgcggtat ggtgataggt gcgattggtt gcgccccagt aaccaagcag cagattcatg
     4921 tccatgaacc agctgtgcac cttgtccttg cggttcctaa cctgctcgac cacccgttcg
     4981 ttgaaagaaa tgggtgacaa gcctggcgtg caagacaaac atttctggct ggccgaataa
     5041 accgcgtcaa ttccccattc gtcaacaagc actggtgtgc cgccgaggga tgtcaccgcg
     5101 tccataatgg taagggcacc gtacttacgc gcgatctcgg ccagcgtttt ggcatccgag
     5161 gcgacaccgg tagaggtttc tgcatgcacg aatgcgagga tcctggcatc aggatttgct
     5221 ttgaaggcgt cttccacttt ctgcgcgctg acgggagtgc cccagcggtc ttccacgacg
     5281 accgcgatgc cgccgcagcg ctgcacgttt tcgatcatgc gcccgccaaa cacgccgttg
     5341 cggcaaacga ttactttgtc tcccggggtc accatattga cgaagcacat ctccattccg
     5401 accgatcccg ggcccgaaac gggaaaagtc agcgcattgt tggtctgaaa tccgtagcgc
     5461 aacagttcct tgagttcctc catcatttcg acaaataccg ggtccagata gccgatggtc
     5521 ggcttaccca tggcggaaaa cacgcgcggg tggatctccg aaggtcccgg ccccattaga
     5581 tggcgttgcg gcggataaaa ggaattgatt tttctctttg gagcgtaatc ttgggtggac
     5641 atgcggttcc cgaggtagag gattgaaacg gaggccgcgg tacggccaaa aagaagcgca
     5701 gtgtactaga ccgccggggc accggccaag caggccccca gtctcactgc ggtatatccg
     5761 ctgtggcgtt ggctggattt ccgaattgat gcgccccaag ctgaaagccg gggctcactt
     5821 ttcacccacg gctggaacag gaggcggccg tgttactcgt ttgccgattt ggcagcggga
     5881 aactggatca ggcgcgcgga aaaatcttcg ctgtcgaatg caatatcgcc atccttgtag
     5941 gcttccggct gcgtcttgag gttggcgaaa tcgaattgcc gcggatcgag cagatgtgaa
     6001 ggtgaagtgc gttgcaggct ggcgaacatg gtttccaggc ggccggggaa tttcctttcc
     6061 cactcctgca gcatgttctt gaccacctgg cgttgcagat tctgctgcga gccgcacagg
     6121 ttgcacggga taattgggaa ctggcgcagc ctggcatact cctccaaatc cgcttccttg
     6181 caataagcga gcggacgaat gacgatgtgc tggccatcgt cgctgatcaa ctttggcggc
     6241 attgccttca ggcgcccgcc atggaacaag ttcagaaaaa aggtttccag aatatcgtca
     6301 cggtgatggc cgagcgcgat tttgttcgca ccgagttccc gagccacgcg atacagcgcg
     6361 ccgcggcgca gccgcgaaca caatgaacac atggttttgc cttccggaat gacgcgcttg
     6421 accacactgt gcgtatcctg ttctacgatc cgaaacggca cgccgatcga agtaagataa
     6481 tcgggcagca ctgctgccgg atagccggga tgtttctgat caagatttac tgcgatgatc
     6541 tcgaaatcga tgggcgcatg attcttcagc gacaacagta catccagcat cgcgtaactg
     6601 tctttgcctc ccgacaaaca caccatgacc ttgtcgccat tctggatcat gtcgaagtgg
     6661 ccaacggctt caccaaccag acggcgcagc cgtttggcca gtttgttgcc ttcatagacc
     6721 tgtctctgaa ctgttgtcat ttagcgggta cggaaaatct caaaaccaac tgattcacaa
     6781 tccttgtaaa catccagctt ttgactggag acgcgggccg cccggacgcc gggacgcgcc
     6841 agacagatgg ccagaatccg ctcgcaaaaa gtctcctgca gattgaagtg gctgctgtcg
     6901 gcaaggccga ctatttcacg ccgaacgaaa tcatagtcta ccgtttcatc gatgtgatcg
     6961 cgttcgatct tgccgtccgg atcaaggtac agatccacgt ttacgaccac gcgctgcttc
     7021 gcccgtttct cgaagtcatg gataccaatg gaaagacgtg tttcgaagtt tttcagaaaa
     7081 atgcggcgcg tggggcaggg gggcattgca gacgcctcgc cggtcaggtc tctccgctgg
     7141 tccaggttgt cgctcattgg tcattaccaa ctgcaaactg gacatcccgc cgcgatggcc
     7201 acaagtgctg cccgccgtcc accacgatgg tggtgccagt cagcgcaccg gcgttgacga
     7261 caaaacgcac cgcatcggcg atatcttcga cagtggaact gcgtcccaag ggtgccatgc
     7321 gatgggagcg tgcgaatccc tccgcagtct gctcgccact caccagggtg atgccgggtg
     7381 cgacaccgca gacgcggacc ttgggggcga gcgccagcgc gagcagccgc gttgcgccct
     7441 cgaggccgat ttttgcaacg gtataggaca gaaaatccgg attgagattg aatactttct
     7501 ggtccagcat attaacgatg cagccagtca gctcatcggg caagcgtgcc gcgaacgtct
     7561 tcgacagcaa cagcggcgcg aacagattga cgtcaaggtg ccgatgccac tgtgcggtct
     7621 ccaggctcgc gagatcgtcg taggcaaata ccgcggcgtt gttgaccagg caggtcaccg
     7681 tgccgagatg gcgctcagat gcctcgacca actccggtac ctgttctgca tcgctgaggt
     7741 cacagcgaac tgccaccgct tttgcgccga gcgctgaaat ttccgccgac aggcgctctg
     7801 cttccgcctc cccgcgatga aaatgaagcg cgatgtccca gccgtccgcg gccagggcaa
     7861 gtgaaattgc ccggccaatg cgtttgccgg ctccagtgac cagggccacc cgctggtgct
     7921 ttttcgcccg cgccatggtt cgcttgaggt tgcgccgtac cgcgcagctg ttgttgaatg
     7981 ggaattgtag cccaaccgtt tcgcatttca ccttgcctgc tgcttcgcgc cccattgact
     8041 tccctgaacc ttcttcggaa gcgcgccgcc acagcgaacg gctgagcgac agcatccgca
     8101 atgaaatcgc cgcgtcaggg ggctggatca gcttcgcacg cttcatggaa atggccttat
     8161 acgcgccagg acagggctat tacgtcgcgg gtactgcgaa attgggagaa gcgggggatt
     8221 tcgtgacggc acccgaaata tctgcgctgt tcggcaggac tttggcacac caggtggcgc
     8281 aggtgctcga gttcagtggc gcagacgccg ttctggaact gggcgcaggt tcaggcacaa
     8341 tggcagctga tgtgctcggc gaactacgca acatcgatcg gcttccgcgg cgttatgtga
     8401 ttcttgaaac cagtccgcag ctggtgcagc gccagcgcga cacgctgtgg cgacgccagc
     8461 ccgcactcaa tgaccggatt cagtgggtca ccgcattgcc cgaaaatctc gatggcgttg
     8521 tcatagccaa cgaggtactg gatgcgcttc cggttcacct cgttgcttgg cgccgggacg
     8581 gactgcatga gcgtggcgta agctggaagg acagttttgt atgggaagag cggctgttgc
     8641 aaccgggtgc tctccgaagc gccgctgaag cgattggcgc cgcgcccgat tacctcagtg
     8701 aaatttcatt acttgcgccg ggcctggtac gcagtttgtc gaataccgtg cgccgaggcg
     8761 cgctgctgct gattgattat gggtttggct ggcgcgagta ctaccatccg cagcgttcgc
     8821 gcgggacgct gatgtgccat taccgtcacc tcgcacacga cgatccgttt tttcttcccg
     8881 ggttgcagga cgtcactgcc cacgttgatt tcaccgcgat tgcgcaagcc ggcatggagt
     8941 ccggaatgga tctcctcggc tataccacgc aagcgcagtt cctcattaac agcggaatca
     9001 tcgggtggat ggagcaacag ccggaacgat catcggcggc acgcattgca cacaccgcgg
     9061 gcgtgcagaa actgctgtca ccggcggaaa tgggtgagct gttcaaggtg attgccttgt
     9121 cacgtggtat tgaaggtccg cttatggggt ttacctctgg cgacaaatcg cggctgttgt
     9181 gaagtccgcg cgcaactctg caggtttgta ttttgaagtg gttttgcgta aggttgcgcg
     9241 tcgtttgatc ttgcaccatc tgactgcgcc ttcgcgcgac acaggctata gctgaaaatg
     9301 cacatccaca ttctgggcat ctgcggtacg ttcatgggcg gaattgccgt cattgccaga
     9361 caggctggcc acaaagtgac cggatgcgat gccaacgtct acccgccgat gagcacgcaa
     9421 ctgcaggcgc agggaatcga actgatcgag ggattcgacg ccgggcagat cgacttgaaa
     9481 cccgaccttt ttgtgatcgg caatgtcgtt tcgcgcggta atccgctgat ggaacagatt
     9541 ctggatcgag gactggcatt tacttccggg ccgcagtggc tgtatgaaaa tatcttgcgt
     9601 ggcaagtggg tgcttgcggt cgccggcacg catggtaaaa ccacgactac atcgatgctg
     9661 gcctggattc tggaacacgc agggctcaat ccgggattcc tgatcggtgg cgtgccattg
     9721 aattttggta tatctgcccg taccaccgat tcgtcgttct tcgtgatcga ggcggacgaa
     9781 tacgacaccg cattcttcga caagcgctcg aagttcgtcc actatctgcc gcgcacggcg
     9841 atcctgaaca atctggagtt cgatcacgcc gacatttttc cagacctggc ggcgattgag
     9901 acccaattcc accatttggt gcgcacgttg ccgggcagtg gtttgattat cgcgcacggg
     9961 cgcgaacaaa gcatgcgccg cgtgctggcc aagggcgtct ggacgaaagt ggagtggctg
    10021 ggaattgaac agggttggac ggcgcggcca cgcgatgcac acgcgttcga cgtattcttc
    10081 gaaggcggac cacggggcac agtgaactgg gagctactcg gcgatcacaa ccgtaacaac
    10141 gcgcttgctg ccctggcggc tagccgtcac gcgggagtac cggtcgccaa gggaatagac
    10201 gcattggccg agttcaggaa tgtaaaacgc cggctagagg tcagtggcgc ggtcaatggc
    10261 gtcaccgtgt acgacgattt cgcccatcat ccaaccgcca tcgaaaccac cattgcgggg
    10321 ctgcgcagca aggtcggcaa tgcgcgcatc ctggcggtgc tcgagccacg ctcgaacacc
    10381 atgaaaatgg gcatcatgaa agatgctctg gctggcagcc ttgccggagc ggacctggtg
    10441 ttctgctata ccaacaacct cggttgggat gcgcaaccgg tgctggcatc ccttggggca
    10501 aaagcacagt gcttcgagga tctgggtgca ctggttgcgg ccatcgtggc gcacagccag
    10561 gcgggtgact acgtgctaac tatgagcaac ggtgcattcg gcggcatcca tgatcgattg
    10621 ctggaagccc ttgcgaagta agccagtcgg cgatcaagac cgacatcagg cgtcgcctgc
    10681 ccgaggggtt cacgccgtcc agagactctt gggcaccagg caatgcacgg tcttattgaa
    10741 ttcgccgata tcgcccaggc gcgaatccat gccaaggctc atcagcgaat aggcatcaat
    10801 gactgacatg tttttgcgtt cggcaatctg ctcgatcatc gcaaaggcgg cctggttcat
    10861 ggcgctttgc aggtccggcg atgacgcgct ggttacaaaa taatgtggcg tctcccgcag
    10921 cgggcgcacc gcgcgcttca acttgacgcc agattttgga atcatgcaga acacgccttt
    10981 catctgattg acgacttccg agatgcggca gtcatagcgg ctcgccatcc attctgcggt
    11041 tttctcggga tgaatatcct gcgactcggc caggaacgta ctggtttccc gcttcagtat
    11101 ttccagagcc ttgttcaagt cgcgatcgta gcccacggtg atccagtgtg tcggcgtttc
    11161 gatgcgcggc cactccagtg acttgccttt aagcacgtca acccgcaggc gcagctcatt
    11221 aaatgcggtt tcgatggcgg tcaggttgat ttcgccgttg ccctgtgccg cgtgcgaatc
    11281 gccgctccac aacagcgcgc cttcaacgaa taccggcaaa tagacggtcg tgccttcgat
    11341 catctctcgc acatccatgt tgccgccgaa aggacccggt ggcactgtgc tgtaccgacc
    11401 ggcttcagcg cgcgcaacac ccaagacgcc ggggaaagga cgcagcggta tccgcactcc
    11461 gggggcgaac tgcgtgcttg ctgtttgctg atcaaagtaa aaataacgaa cctgaccggc
    11521 cggaaaccga tcgggaaact gtcccagacc gagttcgcca ggcagattcc agttggctcc
    11581 gtagctcctt ggcaagatgc gttcaatatg gactttcagc gtgtcgcccg gctcggcatc
    11641 ctcgatgtag attgggccgg tgatggtatg cgggccgcgc ccggggaaag ccagtcgcat
    11701 ctgggttatg gcttcgatcg atacgcccgg caacacctgg tttgcggacg ccatcatggt
    11761 ttccatcgaa atgcgcgcgc ccgagcggac cgtcagtacc ggaggctcgg cattgtcgaa
    11821 ccagccccat tgcgtcgtgt ggggtgttgc cggcaacacg tggatgtccc cgagcattgc
    11881 gccgggcgga accgggcgcc tttgctgctc ctcggctttg agtacatccc ccagccaggg
    11941 ggcactcgcc actgcccccg actgcagaaa aaatcgcctg cgggaatcat cagtcattgg
    12001 aaatccttca ttggtcgact tgcggttttg tggcaggcat ttttgcgcaa tttcgcagac
    12061 gacacgcagc ttctttgtcg agccggttgc cgtacggcac aatgccggtg caacggaatg
    12121 tcttcatgat catctatatt cacggcttca acagttctcc gtcgtcccac aaagccaacc
    12181 agttgcgtga gcgtttgaca tcgttgggcc gcgcacacga gtttttttgc ccggcgttgc
    12241 cgcactggcc agcgcaagca atggcactgc tcgaagggct ggttagcgcc actccgccgg
    12301 ctgacgttac gctggtcggc agttcgctgg gcggattcta tgcaacctgc cttactgaaa
    12361 aatatggagc gcgatcggtg ctggtcaatc cggcaattac gccccatgaa ggtttgcgaa
    12421 gctatctcgg ccagcaaaaa aacctctata gcggcgacga atatctactg accgaagagc
    12481 acctgacgca actggcccgg ctgcatgtgg cgcaaccggc cagcatggac cgctacctgc
    12541 tgatgcatac caccggtgat gaactgctgg actggcgaat cgcggttgac cattatcgcg
    12601 gctgccgcca agtgatcgtg aaagggagcg atcacggttt cagtgaattt gcccagtaca
    12661 tcgacatcgt actgagcttc gccgaccgct cttaggaaat gtcgagcctt gcctttgcgg
    12721 attgccccgg gcgaagcgat gtacgctctc acgtataatc gcgccctgct tccacccgcg
    12781 taatccccgc ctccccgggg cagcacatga acgttttttt cgaagaagac ggcgcattca
    12841 aggccggcac tgtgctggcc gacaaccata cctccctgca ggtggaaaat cagcacggca
    12901 agcgcacgaa ggtgaaggaa ggcgctgtgc tgatccggtt cgatcatatt ccgctgtcgc
    12961 aattcatggc ggaggcgcaa agcatcgcaa aggacatcga cccggcgttt ctttgggaat
    13021 gttccggtgc aactgaattc ggattcgatc aattggggaa agaatatttt ggacatgagc
    13081 ccagaccggc agaaagtgcc ggactgctga tgcggctgca cgctacgccg acgcacttct
    13141 acaagaaagg caaaggtcgt tacaaggccg cgcccgccga cgcattgcgg gccgcgcttg
    13201 ccagcgtgga acggaaaaga aagcaagccg atctgcagca gcggtatgca gagcagcttg
    13261 cgcgcttcga agttcccgag gagttcaagc cattgctgcc gcaactcctt taccggcctg
    13321 ataaaggatg catcgaatac aaggcgctcg aacaggctgc tgcgctggcg catctgacca
    13381 ttccgaggct gctggaaaga agtggtgcgt ttgctt
//
LOCUS       DATMZQ010000508        18199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_3782257, whole genome shotgun sequence.
ACCESSION   DATMZQ010000508 DATMZQ010000000
VERSION     DATMZQ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18199
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782257"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2206)
                     /gene="topA"
                     /locus_tag="VN496_16190"
     CDS             complement(<1..2206)
                     /gene="topA"
                     /locus_tag="VN496_16190"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXF18550.1"
                     /translation="MSQSLLIVESPSKAKTLKKYLGRGFEIVASYGHVRDLEAKEGAV
                     DPANDFSMRYALVEKNKKHVDAITRAVAKATNIYLATDPDREGEAIAWHLAEILKSKK
                     LLKNKKLGRVVFYEITESAVKSAVAHPRAISMSLVNAQQARRALDHLVGFNLSPLLWK
                     KIGPSLSAGRVQSPALRLIVEREHEIETFKSREYWSLHLDSHKGKQTFSARLIQHKGT
                     KIEQFSIGTDSEQRAIVSEIEKAARGTAQVVKVERKPKSRQPAAPFTTSTLQQEAVRK
                     LGMTTRGAMSTAQQLYEGVEIDGGTVGLITYMRTDSLALAKEALVEIRNYVKKKFEPA
                     YLPQSAVEYRNKSKNAQEAHEAIRPTSIERTPDAVRKYLTPDQAALYEMIWKRTLSCQ
                     MTPAKYDTTSVDLTAGRTDTVFRATGQILVFAGFMAVYLEDDDDAEQEQESKLPRLEE
                     KESVPIDRIFGEQHFTQPPPRYTEASLVKTLEEFGIGRPSTYASIISTLQDRGYALLD
                     KKRFMPTDIGRLVNGFLTSHFNHYVDYGFTALLEEELDDISNGDREWVPVLKDFWKDF
                     ARTLKSKEKVARGSPMDEACPTCGKQLFLQNSRRGLFVGCSGYPNCDYTRPWGDQPAG
                     PILLGKDPVTDSDILLLKGPYGFYVQLGQTPEDKSQKPKRAAWPKNVPASSADLDTAL
                     KVLALPRQLGTHPESGKMVEANIGRFGPYVKHDGTFKSIPKSEDVHDITLTRA"
     gene            complement(2281..2739)
                     /locus_tag="VN496_16195"
     CDS             complement(2281..2739)
                     /locus_tag="VN496_16195"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HXF18551.1"
                     /translation="MFDILIFLFENYFHAGTYPDQDILSKRLSDAGFDSRDISQALTW
                     LSGLERVGKNSSRSPSIPSKGFRFYTEPEINTLPTEARGFLMFLESAGVISVLQRELI
                     IERVVALDEPSVDLDKVKLIVLMVLWNQNETLDTLVLEELISPNQEHHLH"
     gene            complement(2773..3855)
                     /gene="dprA"
                     /locus_tag="VN496_16200"
     CDS             complement(2773..3855)
                     /gene="dprA"
                     /locus_tag="VN496_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXF18552.1"
                     /translation="MSPDSETLAWLKLALVAELTPTIFRKLLAAYGTPEAICALDFGT
                     LARNVPDATAAAIVRGPGAECLDVTLHWLEDPANNVVTFADKSYPRLLLEISDPPPLL
                     YVKGNADWLDHASLAVVGSRNATPQGNANAQAFAHELSDGGFTIVSGMALGIDGAAHL
                     GGLSGASSSVAVVGTGLDIVYPARNRELAHRLALEGALVSEFALGTPALASNFPRRNR
                     LISGLSRGCLVVEAALKSGSLITARYALEQGRDVFAVPGSIHSPLSKGCHLLLKQGAK
                     LVENSRDILEELGVASYKSREVSTEGAPAAGNMVLAQLGFDPLDLDVLCQRCGLTPET
                     ASAMLLTLELEGVVNRLPGGKFQRVR"
     gene            complement(3871..4947)
                     /locus_tag="VN496_16205"
     CDS             complement(3871..4947)
                     /locus_tag="VN496_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXF18553.1"
                     /translation="MGKSIISLLVVVAAWSFGGAVADEIKLAESAPDRYIVIKGDTLW
                     GISTKFLKDPWRWPDIWGLNKEEIKNPHWIYPGDVVVLDFTGRTPRLRKEGGRDGEGS
                     DGADWRLMEAKLSPAIRKQPLGAGAISSIPASAIQAFLVKPLVVGDQELSAAPTLVAG
                     PENRVVLSAGDTAFAKGLVWESDPGWSIFRPGRTFTDPDTNEVLGREAVYLGDAQVSE
                     FGEVSTVLVTKATREIAPGDRLARTPPAPALAYVPHAPAPQLKGRVMAGPEGRFSEIA
                     SLQVVVINRGRREGIEVGHVLALYRDRPSVTPAYVSDQKEKIRLPPERYGMVFVFRVF
                     EKLSYALVMNSTQPVNVLDVVQAP"
     gene            5065..5568
                     /gene="def"
                     /locus_tag="VN496_16210"
     CDS             5065..5568
                     /gene="def"
                     /locus_tag="VN496_16210"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXF18554.1"
                     /translation="MAILPIIHYPDPRLHKFAAPVHEVDESIRRLAKDMAETMYAAPG
                     VGLAATQVDVHKQVIVIDISETLDQPLFFINPKIIEARGRADCEEGCLSVPGIFEKVS
                     RAEWVKVQALGIDGQPFEMQSDGLLAVCIQHEIDHLKGKVFVEYLSQLKQKRIADKLK
                     KQQRRAL"
     gene            5568..6497
                     /gene="fmt"
                     /locus_tag="VN496_16215"
     CDS             5568..6497
                     /gene="fmt"
                     /locus_tag="VN496_16215"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXF18555.1"
                     /translation="MRVVFAGTPAFAATALQALIDAGFDVVLVLTQPDRPAGRGLRPS
                     FSAVKQQAIKHRLAIDQPSSLRGEELLARLGALAAPVMVVAAYGLILPAAVINLFPIG
                     CINIHASLLPRWRGAAPIQRAILASDRETGISIMRMDQGLDTGPVYLTEALPMLPDDT
                     AGSLHDRLAGLGARCIVEALPRIECGRLVPAPQPSIGITYAQKITKQEAAIDWSHAAF
                     AIDRQVRAFNPVPGAYSALGRVMIKIWRVQPSNEGQGDPGQITHAGEDGIDVACGQGM
                     VKILELQQAGGKRLVAADFVRGAPTLVGRRFES"
     gene            6508..7773
                     /gene="rsmB"
                     /locus_tag="VN496_16220"
     CDS             6508..7773
                     /gene="rsmB"
                     /locus_tag="VN496_16220"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HXF18556.1"
                     /translation="MHRIQRIAAQTVTIVLAGTGLNTALETAWRSYPQLTASERGAIQ
                     DICYGTLRHLGKLQAVLGLLAPKPIVIAELRTLLLTALYRLEYSKAAPYAVVNQAVEC
                     AVQFGGGHVRSFANAVLRNYLRRREDLLARVSACDEGRLSYPNWWIKKMRIAFGPEAD
                     DILEMGNHQPPMTLRANVRQGGAAEYMQRLNNAGLEARQLENGALVLAKPVAIGRLPG
                     FGDGAVSVQDAGAQFAGPLLDAKDGMRVLDACAAPGGKTAHILEIADVAMTALDADAV
                     RLQRVASNLERIGLRARLQVADAGAPETWWDGKPFQRVLLDAPCSASGVVRRHPDIKW
                     LRRLSDIAGFAIKQRRLLDALWKVVARGGKLLYVTCSIFEEEDQQIIAAFATCHANAH
                     ILGGMPGRNGLLLPANDHDGFFYALLQKS"
     gene            7775..8326
                     /locus_tag="VN496_16225"
     CDS             7775..8326
                     /locus_tag="VN496_16225"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HXF18557.1"
                     /translation="MLHKLISLCLLWCSVTAAASEGIHVKSAEVSQNAGEYYLEANFE
                     VELTHTLEDALNKGLPLHFIVEFELIHPRWYTLYLWNKSILEFEQDYRLSYNALTRQY
                     RLSLGALHQNFDSLYDALALMGRLRPRLLADSDLLDEGKVYEAAIRMRLDITQLPKPF
                     QINALASRDWNLVSEWFRWTLTR"
     gene            8323..10521
                     /locus_tag="VN496_16230"
     CDS             8323..10521
                     /locus_tag="VN496_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXF18558.1"
                     /translation="MKLPRSVRRSLLSRGMQFLIVLVLTLGAVMLYLLSGASANTPWF
                     STDLPLLLALGVGAVLILMLVVGYQLLLLRRRLNARVFGSKLTLRLVLLFALVAVLPG
                     VLVYAVSVQFLSRSIESWFDVRVEKALEGGLSLGRANLDNMLKELRRKAESMAFSLSE
                     RSTPRKLATLNTLREQASVNEATLLRTDGSLISFSSADSTSLVPDLPTPSVVRKVRMQ
                     EPYSTIEALGDGGLQLRVLVPVNDFTGATLLLQLVQPVPAALARDAQTVQEIYRDYQE
                     LSLSRLGLQRLYGLTLTLSLLLALLSALLLAIVFSERLSAPLGVLAAGTRAVAQGDFS
                     QRHLVKSNDELGSLTESFNIMTMQLADAQADAQRNQQQLTAAKAYLESVLGNLSAGVM
                     SFDAEFRLHSANRSAEQILGVSVGELIGSHPDEWANREPSLAAVGNALREALVKSRDE
                     TWEAQVELSGPTGQQVLLLRGSKLSADTEPGMVMIFDDITRMRQAQRLAAWGEVAQRL
                     AHEIKNPLTPIQLSAERLQHRLADKLSSQEAEILQRSTQTIVNQVAALKAMVDAFSVY
                     ARSPVPDLQPLDLNVLTREVLGLYESSRPALQFEFAPSLPLVSGDTAKLRQVIHNLLQ
                     NAEQAVSEVPVPRIVVRTEATDAGVQLTMRDNGPGFPENVIGRVFEPYVTTKSKGTGL
                     GLAIVKKIVEEHNGRIVVANNLSGGASVTILLPAAPGKSTGMRLIRQVGI"
     gene            10518..10859
                     /locus_tag="VN496_16235"
                     /pseudo
     CDS             10518..10859
                     /locus_tag="VN496_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            10905..11318
                     /locus_tag="VN496_16240"
                     /pseudo
     CDS             10905..11318
                     /locus_tag="VN496_16240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11592..11777
                     /locus_tag="VN496_16245"
                     /pseudo
     CDS             11592..11777
                     /locus_tag="VN496_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            11851..13836
                     /gene="tkt"
                     /locus_tag="VN496_16250"
     CDS             11851..13836
                     /gene="tkt"
                     /locus_tag="VN496_16250"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXF18559.1"
                     /translation="MATRNELANAIRALAMDAVQKANSGHPGMPMGMADIAEVLWNSY
                     LRHNPANPGWADRDRFVLSNGHGSMLLYALLHLTGYALPMDQIRRFRQLHSMTPGHPE
                     LGITPGVETTTGPLGQGLGNAIGMALAERLLAAEFNRPGFAIVDHRTYVFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNGISIDGHVDGWFTDETPKRFEAYGWHVVRNVDG
                     HNAAAIQAAIEAGKKILDKPTLLCCKTVIGKGAPNKAGSHDSHGAALGEKEVAATRAN
                     IGWSYPAFEIPADIYAGWDARTKGAASEAAWNAGFDSYAKTFPHEAAEFKRRMSGELP
                     ESWREHADRFVANVQAKAETIASRKASQNAIEALAPALPELIGGSADLTGSNLTLWSG
                     AKAVTRSTGGNYVYYGVREFGMSAIMNGLTLHGGLIPYGGTFLTFSDYSRNALRMAAL
                     MKVRTLFVFTHDSIGLGEDGPTHQSVEHAASLRLIPNMDVWRPCDSVESAVAWIAAIE
                     RKDGPSCLLFSRQNLPFQMRDARAINQIARGGYVLSDSANAKAIIIATGSEVALAVAA
                     QKLLATDGIGVKVVSMPNTNVFDRQGASYRESVLPKNLRRVAVEAGVTDYWHKYVGLD
                     GEVVGLDRFGESAPAGELFRFFGITAEAVADAVKRVI"
     gene            13879..14826
                     /locus_tag="VN496_16255"
     CDS             13879..14826
                     /locus_tag="VN496_16255"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF001416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HXF18560.1"
                     /translation="MDKAMRLVVDIGGTNARLALVPPDSLLPQQEKRVRCADFDSLEH
                     AVQHYLQECGDPAVREAALDVATGITGDFVHLTNGPWGFSIDQTRRALRLDTLHVVND
                     FTALALAIPLLLPHELQQIGGGSAVPETAIAVIGPGTGLGVSGLIPVGGRWIPLQGEG
                     GHTAFSPIGDEEIGVLRWLQQRYNHVSTERVVSGMGLENLYQALCAMAGVQAKPLSPD
                     KITASALSNTDVQCKAALQMFCAILGTAAANLVVTLGARAGCYIGGGIVPRLGAYFAH
                     SAFRSRFESKGRFSSYVAAVPTYVILGETPALRGLATLF"
     gene            14843..15109
                     /locus_tag="VN496_16260"
     CDS             14843..15109
                     /locus_tag="VN496_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HXF18561.1"
                     /translation="MDGLITGIGLLAGLLTTAAFIPQVWKIYQTKSGKDISGRMFWLF
                     SIGIALWLVYGMLLSSIPLIASNVVTLVLSLTILALKYRYSRAT"
     gene            15321..16334
                     /gene="gap"
                     /locus_tag="VN496_16265"
     CDS             15321..16334
                     /gene="gap"
                     /locus_tag="VN496_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXF18562.1"
                     /translation="MTIRVAINGYGRIGRNILRAHYENGKKYPIEIVAINDLGDANIN
                     AHLTKYDTAHGKFPGTVAVDGNNLVVNGDQIKVCAQRSPADLPWQDLRVDVVLECTGL
                     FTSKAKAGAHLTAGAKKVIISAPGDKDVDATVVYGVNHGTLRPAHTVISNASCTTNCL
                     APLVKALNDRIGVVNGLMTTIHAYTNDQVLTDVYHTDLRRARSATMSMIPTKTGAAAA
                     VGLVLPELNGKLDGFAMRVPTINVSIVDLTFVAKRATSVEEVNSAVKQASEAELKGIL
                     EYNKDPLVSVDFNHNPASSVFDSTLTKVSVGTLVKVCSWYDNEWGFSNRMLDTTVAFV
                     NAK"
     gene            16414..17616
                     /locus_tag="VN496_16270"
     CDS             16414..17616
                     /locus_tag="VN496_16270"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXF18563.1"
                     /translation="MMTILRMTDLNLRGKRVFIRADLNVPQDKSGYITDDTRIRASLP
                     AIRMALDAGAAVMVTSHLGRPVEGQFKPEDSLAPVAQRLSQQLGVPVPLKANWVNGVE
                     IEPGHIVLLENCRCNKGEKKDDDGLARKMAALCDVYVNDAFGTAHRAEATTHGIARYA
                     AVACAGPLMAAELDALGKALRQPKRPLIAIVAGSKVSTKLTILAELAKKVDQLIVGGG
                     IANTFMLAAGLSIGKSLAEPDLAGEARSIMQTIKSKGGAVPIPVDVVCAKEFSAEAAA
                     TIKDAQQVAADDLILDIGPRTAAQLAALLGRAGTIVWNGPVGVFEFERFGEGTRVLAQ
                     AIADSPAFSIAGGGDTVAAIAKYGIGDRIGYISTGGGAFLEFLEGKKLPAVEILESRA
                     AAQPQERA"
     gene            17613..>18199
                     /locus_tag="VN496_16275"
     CDS             17613..>18199
                     /locus_tag="VN496_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXF18564.1"
                     /translation="MTQKSTKIVATLGPASSDRDVLMRMIAHGVDVVRMNFSHGTKEE
                     HLARAELVRDVARSLERTVGIMCDLQGPKIRIGKFEAGRVTLNKGETFTLDADCPVGD
                     QHQVGLDYKELPHDVGAGAVLLLDDGRLVLDVQKVQDSRITTTVRVGGVLSNNKGINR
                     QGGGLTAPALTAKDMEDIKTAVAMRADFLAVSFPK"
BASE COUNT         3882 a         5054 c         5258 g         4005 t
ORIGIN      
        1 cagctcgtgt cagcgtgatg tcatgcacgt cctcggactt gggaatcgat ttaaaggtcc
       61 cgtcgtgttt aacatacggg ccaaaccgcc cgatattggc ctcgaccatc ttgccagact
      121 cgggatgcgt gcccagctgg cgcggaagcg ccaatacctt tagcgcggta tccaagtcgg
      181 ccgaagaggc aggaacattt ttcggccatg cagcgcgttt gggtttttgc gatttgtctt
      241 ccggcgtctg tcccaattgc acatagaacc cgtacggccc tttcagcagc aagatatcgc
      301 tatctgtcac cggatctttg ccgagcaata tcggaccagc cggctggtct ccccatggac
      361 gagtgtagtc acagttggga tatccgctgc agccaacgaa cagtccgcgc ctgctgttct
      421 gaagaaacaa ctgctttccg caagtggggc acgcttcatc catcggactg ccgcgcgcaa
      481 ctttttcctt cgattttagc gtgcgggcga agtccttcca gaagtctttg agcaccggaa
      541 cccattcacg atcgccattt gaaatgtcgt cgagttcttc ttccaaaagg gccgtgaaac
      601 cgtagtcgac gtagtgattg aaatgtgaag tcaggaagcc atttacgagc cggccgatat
      661 ccgtgggcat gaaccgcttt ttgtcgagca atgcgtaccc gcggtcttgc aatgttgaaa
      721 taatgctggc ataggtggac ggtctgccga tcccaaattc ctcaagcgtc tttaccaggc
      781 ttgcttccgt gtaacgcggc ggcggctgtg tgaaatgctg ctcgccaaat atgcggtcaa
      841 ttggcacaga ttctttttct tccagccgcg gcagctttga ttcctgttcc tgctcggcat
      901 cgtcgtcatc ttcgaggtag acagccatga agcccgcgaa aacgagtatt tgccctgtcg
      961 cacggaacac tgtatcggta cggcctgccg tcaaatcgac ggaggtcgta tcatatttgg
     1021 ccggcgtcat ttggcatgac agcgtgcgct tccagatcat ttcatacaga gcggcttgat
     1081 cgggcgttag atatttgcgc actgcgtccg gggttcgttc tattgaagtg gggcgaattg
     1141 cttcatgcgc ttcctgcgcg ttcttggatt tgttgcggta ctcgaccgcc gactgcggaa
     1201 ggtaggcggg ctcgaatttt ttcttgacgt aattccggat ttcgacgagc gcctccttgg
     1261 ccagagccag cgaatcggtt cgcatgtagg taatcagtcc aaccgttccg ccatcgattt
     1321 ccacgccctc atacaactgc tgggcggtac tcattgcccc tcgcgtggtc atgccaagct
     1381 ttcgcactgc ctcctgctga agcgtcgatg tggtaaatgg cgctgccggt tgcctgctct
     1441 tgggcttgcg ctcgaccttg acgacctgcg cggttcctct ggctgccttt tcgatttcag
     1501 aaacgattgc gcgctgttca ctgtcagttc caatggagaa ctgttcgatc tttgttccct
     1561 tatgctgaat caaacgcgcg ctgaacgtct gtttgccctt gtggctgtcg agatgcagcg
     1621 accaatattc gcggcttttg aaagtctcga tttcgtgctc gcgttctacg atcagtcgca
     1681 gagcaggact ttggactcga cccgcagaca ggctgggacc aatctttttc cagagcaatg
     1741 gcgagagatt gaaaccgacc aagtgatcga gtgccctgcg cgcttgctgc gcgtttacca
     1801 gcgacatgga gatcgcgcga ggatgcgcaa ccgccgactt gactgccgat tcggtaatct
     1861 cataaaacac aacccgtccc aattttttgt tcttcagcaa tttcttgctt ttcagaattt
     1921 ccgcgagatg ccaggcaatc gcctcaccct cgcggtccgg gtctgtcgcg agatagatgt
     1981 tggtcgcttt tgctacggcc ctggtaatag catcgacgtg ttttttgttc ttctccacca
     2041 gcgcataacg catgctgaaa tcgttggccg ggtcaacagc gccctccttt gcttccagat
     2101 cgcgcacgtg accatatgac gcaactatct cgaagccgcg gccaagatat ttcttaagag
     2161 tcttggcctt ggagggcgac tctacgatta gaagactttg agacattgaa cttccggcct
     2221 gaaaaggagg cggaatgata agaacttgat ggcgctggag caagcgccat gatcaagcgt
     2281 ctagtgtaag tggtgttcct ggttgggcga aatcagctct tccagaacca acgtatcgag
     2341 cgtttcgttt tgattccaca acaccatcaa aacgatcaac ttgaccttgt cgagatctac
     2401 ggacggctcg tccagcgcta cgacacgttc tattatgagt tcgcgttgca aaacgcttat
     2461 tacgccggca ctttcaagga acatcaaaaa tcctcgcgct tcggtcggca gcgtgtttat
     2521 ttccggttcc gtgtagaaac gaaagccttt gctcgggatg gatggcgagc gcgaactatt
     2581 cttgccgacg cgctcgagtc cgcttaacca ggtgagcgcc tgagaaatgt cgcgactatc
     2641 gaaccctgcg tctgataggc gttttgaaag aatgtcttga tcggggtagg taccggcatg
     2701 aaagtaattt tcgaacagga atatcagtat gtcgaacatg agtcctgtct ggacgggtta
     2761 acgctcgtca cgctagcgca cgcgctggaa ctttccgccg ggaaggcgat tgacgacgcc
     2821 ttcaagttcc agtgtcaaca gcatcgccga tgccgtttcc ggcgtcaagc cgcagcgttg
     2881 acacagcacg tccaagtcca acgggtcgaa gcctagttgt gccagcacca tgttcccggc
     2941 ggcgggcgcc ccctccgtgc tgacctccct ggacttgtaa cttgctacac cgagttcctc
     3001 aagtatgtcg cggctgtttt ctactagctt cgcgccttgc ttgagcagca aatgacaacc
     3061 tttcgatagc ggcgagtgta tcgaacccgg gacggcaaat acatcgcggc cctgctcaag
     3121 cgcataacgt gcggtaatca gcgagccgct tttgagcgct gcttccacca ccaggcaacc
     3181 ccgcgacagt ccgctgatca ggcggttccg gcgtggaaaa ttcgaggcaa gtgcgggcgt
     3241 tcccaaggca aactcggata ccagagcacc ttcgagcgca agccggtgtg ccagttcccg
     3301 gtttcgggcg ggatatacga tgtcgagtcc ggtaccgacg acggcaactg aggaagaggc
     3361 tcctgaaaga ccgcccaggt gtgcggcacc gtcaatacca agtgccatgc cacttacaat
     3421 ggtgaacccg ccgtccgata actcgtgagc aaacgcctgc gcgtttgcgt tgccttgtgg
     3481 cgtggcattg cggcttccta ccaccgcgag agaagcatga tccagccaat ctgcgttgcc
     3541 tttaacgtac aacaacggtg gcggatcgct aatttcaagc agcagtcggg gataagattt
     3601 atctgcaaaa gttacgacat tattggccgg gtcctccagc cagtgcagcg taacatccag
     3661 gcattccgca ccgggaccgc gcacgattgc ggcggccgtt gcatccggga cattgcgcgc
     3721 cagcgtccca aaatcgagtg cgcagatggc ttcaggagtg ccatatgcag ccagaagctt
     3781 ccgaaaaatc gttggcgtta gctcagcaac cagcgcgagt ttgagccaag cgagcgtttc
     3841 ggaatccggc gacatgcgcc gagtacttat ttaaggcgcc tgaactacgt caagtacgtt
     3901 gacgggctgt gtggaattca tgactagcgc ataagagagt ttttcaaaga cgcggaatac
     3961 aaagaccatt ccatagcgtt cgggagggag gcgtatcttt tccttctgat cagatacgta
     4021 ggccggcgta actgagggcc gatcacgata gagtgccagc acgtgtccga cctctatgcc
     4081 ttcccgacgg ccgcgattga tgactacgac ctgaagcgac gcgatctccg agaagcggcc
     4141 ttccgggcct gccatcacgc gtcccttcaa ctgtggcgct ggggcatgcg gtacatacgc
     4201 cagcgcaggg gcggggggcg tcctggcaag tcggtcgccc ggcgcgattt cccgagtggc
     4261 cttggttacc aaaaccgtac tgacttcccc aaattcgctg acttgagcat ctcccaggta
     4321 aaccgcctcg cgaccaagta cttcgttggt gtccggatcc gtaaatgtgc ggccaggcct
     4381 gaaaatgctc caacccggat cgctttccca caccaggcct ttcgcaaaag cagtgtcgcc
     4441 agcggacaat acaacccgat tttcaggccc ggccaccagc gttggagcag cactcagttc
     4501 ctgatcccct actaccagtg gctttaccaa aaaggcctgg atggcggagg ccggtatgct
     4561 tgaaatggcc cccgccccca atggttgttt gcgaatagca ggacttagtt ttgcctccat
     4621 cagacgccag tcagcaccat cgctgccctc gccatcccgc cctccttctt tgcgcagacg
     4681 aggtgtcctt ccggtgaaat caagcaccac tacgtcgcct ggatagatcc agtgtgggtt
     4741 tttgatttct tctttattaa gaccccagat atctggccat ctccagggat cttttaaaaa
     4801 tttggtcgag attccccaca acgtatcgcc cttgatcacg atataccggt caggtgcact
     4861 ttcagcgagc ttgatctcgt cggcgacagc gccgccaaag ctccaggcag caacgaccac
     4921 cagcagagat ataattgatt tgcccattcg aggcccccat ctccggtcaa tccaacaaac
     4981 gattcgcgag cgccgactat tcagcggcgg attggatact ttgcattaaa ttctgcctct
     5041 agttagttaa attagcaagc atttatggca atactcccta tcatccatta ccctgacccg
     5101 agactgcaca aatttgctgc gccggtgcat gaagtcgacg aaagcattcg tcgcttggcg
     5161 aaagacatgg ccgagaccat gtatgcggcc cccggcgtcg gtctcgcggc gacacaagtc
     5221 gatgtacata agcaggttat tgtgattgat atctcggaaa cactggatca gccactgttc
     5281 ttcatcaatc caaagatcat tgaggcccgg ggcagggcag actgcgagga aggatgcctt
     5341 tctgtgcctg gcatttttga gaaggtttcc cgcgcggaat gggtaaaggt ccaggcgctc
     5401 ggaatcgacg gccagccatt cgagatgcag agcgacggcc ttctggcagt gtgcattcag
     5461 cacgagatag atcatctgaa gggtaaggtg tttgtcgaat acctttcgca gcttaagcag
     5521 aaacgtatcg ccgacaagct gaagaagcaa caacggcgag cgctctgatg cgtgtcgtat
     5581 tcgccggcac gccggcgttt gcagctactg cgctgcaagc gctgattgat gctggtttcg
     5641 atgtcgtact cgtgttgact cagcccgacc gccctgccgg ccgcgggctg cggccttcat
     5701 tcagcgcagt aaagcaacag gctatcaagc accgactcgc catcgatcag ccgtcgtctc
     5761 tacggggcga agagttgctc gcgcggctcg gtgccttggc agcaccggtc atggtggtcg
     5821 ctgcttatgg acttatactc cctgctgccg taatcaatct ttttcccatc ggctgcatca
     5881 acatacatgc ctcgctgctg ccgcgctggc gtggagctgc ccctattcag cgtgcgatac
     5941 tcgcctctga tcgcgagacc gggatctcta tcatgcgaat ggatcaagga ttggataccg
     6001 gccctgttta tctgactgag gcgcttccga tgttgccaga cgacaccgct ggttcattgc
     6061 atgatcggct ggccggcttg ggggctcgct gcattgtgga agcattgccg agaattgaat
     6121 gcggccggct ggttcctgcg cctcaaccgt ccatcggcat tacatatgcg caaaaaatca
     6181 ccaaacagga agcagcaatt gattggagtc atgcggcttt cgccattgat cgccaggttc
     6241 gcgcattcaa ccctgtccct ggcgcttaca gcgcgctcgg gcgcgtaatg atcaagatct
     6301 ggcgcgtgca accgagcaat gaaggacagg gggatcctgg ccaaatcacg cacgcggggg
     6361 aagatggaat cgacgtagcg tgcggccagg gcatggtcaa aatactggaa ttgcaacagg
     6421 ccggtgggaa gcggctcgtg gcggcggact tcgtgcgcgg cgctcccacg ttggtgggcc
     6481 ggcgattcga gtcctgatca aaactcagtg caccgtatcc agcggatagc cgcgcaaact
     6541 gtaacaattg tgctggccgg caccgggctg aacacggcgc ttgaaacagc gtggcgtagc
     6601 tacccgcagc tcactgcctc cgaacgcggc gccattcagg atatttgcta cggaacgctg
     6661 cggcatttgg gaaagctgca ggccgtattg ggattactcg cgccgaaacc gattgtaatt
     6721 gcagagctgc gaacgttgct ccttacggcg ctttatcgat tggaatatag caaagccgcg
     6781 ccttacgctg tagttaatca ggcagtggag tgcgcagtgc agtttggcgg cggacacgtc
     6841 aggtcttttg ccaatgcggt tttacgcaat tatttacgcc ggcgtgaaga tctgctggca
     6901 cgagtctctg catgcgacga agggcgtctc tcttatccga attggtggat aaagaaaatg
     6961 cgcatcgctt tcggtcccga ggcagatgac atccttgaga tgggcaacca ccaaccaccg
     7021 atgacgctgc gtgcaaatgt gcgccaaggt ggcgccgcgg aatatatgca gcgccttaac
     7081 aacgccggac tcgaggcccg tcagctggag aatggcgcac tggtgcttgc caaaccggtg
     7141 gcgatagggc gcctgcccgg atttggcgac ggcgccgttt cagttcagga tgcgggcgcg
     7201 caattcgccg gacctttgct ggatgcaaaa gacggaatgc gggtacttga cgcatgcgcc
     7261 gcgcccggcg ggaaaacggc gcatatcctc gaaattgcag atgtcgcgat gacggcgctc
     7321 gatgccgatg cggtacgact gcagcgcgtg gcatcaaacc tggagcgcat cggtctgcgg
     7381 gcgcgcttgc aggtagcgga cgcgggtgcc ccggaaacat ggtgggatgg aaagccgttt
     7441 cagcgcgtgt tgctcgacgc tccgtgttcc gcttcaggag tggttagacg acatcccgat
     7501 atcaaatggt taagaagatt atcggacatc gccggctttg ccataaagca acgccggctg
     7561 cttgacgcat tgtggaaggt agtcgcgcgt ggtggtaaat tactttacgt gacctgttcc
     7621 atcttcgaag aagaagatca gcaaataata gcggcgttcg cgacctgcca cgctaatgcg
     7681 cacattctgg gaggaatgcc aggacgcaac ggcttgctac tcccggccaa cgatcacgat
     7741 ggtttttttt acgccctcct ccagaagagt tgatttgctg cacaagctta tttcgctgtg
     7801 tctgttgtgg tgcagtgtga ccgctgcagc aagcgaaggc atccacgtca agtcagccga
     7861 ggtttcgcaa aatgccggcg agtactatct ggaggcgaat ttcgaggttg aactcactca
     7921 tacgctcgaa gacgcgctaa acaaggggct gccactgcat ttcatagtgg aattcgagtt
     7981 gattcatcca cgctggtata cgctctatct ctggaacaaa tcaatactgg aattcgagca
     8041 ggactatcgt ctttcgtaca acgcgcttac gcgccaatac cgcttgtctt tgggagcgct
     8101 tcatcaaaat tttgactcgc tatatgatgc gctggcattg atgggccgcc tgcggccccg
     8161 gttgctggcc gattccgacc tgctggacga aggcaaggtt tatgaagcag ccatacgcat
     8221 gcgcctcgac attacgcagt tacccaagcc gttccagatt aacgccctgg cttcgcgtga
     8281 ctggaatctt gtctcggaat ggtttcgctg gactctcacg cgatgaaact tccccgcagc
     8341 gttagacgct cgctccttag ccggggaatg cagttcctga ttgtgctggt gctcacgctc
     8401 ggcgcggtga tgttgtatct actgtccggg gcgagtgcca atacaccctg gttttccacc
     8461 gaccttccgc tgctgcttgc actcggggtt ggtgccgtac tgatactcat gctggtcgtc
     8521 ggatatcaat tgctgctgct acgcaggcga ctcaatgccc gcgtattcgg ttcgaagctt
     8581 accttgcgcc ttgtgctttt gttcgcactg gtggcggtgc tgccgggagt actggtatac
     8641 gcggtgtccg tccagtttct ttcacgatcc atcgaatcct ggtttgacgt gcgcgttgaa
     8701 aaggcgctcg aaggggggtt gagcctcgga cgagcgaatc tcgacaatat gctgaaagag
     8761 cttcgccgca aggcagagtc gatggcgttt tctctcagcg aacgtagcac gccgcgtaag
     8821 ctcgccacgc tcaacacgct gcgcgagcag gcgtccgtga atgaagctac gttgctgcgg
     8881 accgatggct ccctcatctc cttctccagc gcggattcga ccagtctggt tccggacctg
     8941 ccaacgccat ccgtcgttcg caaggtaaga atgcaggagc cctacagcac tattgaagcg
     9001 ttgggtgatg gcgggttgca gctacgcgtg ctagtccccg tcaatgactt caccggagcg
     9061 accttgctcc tccagttggt ccagccagta ccagcggcac tggctcgcga cgcgcagacc
     9121 gtccaggaaa tctaccggga ctaccaggag ttgtccctgt cgcgacttgg attgcagcgt
     9181 ctgtacgggc ttaccttgac gctatccctg ctacttgctt tgctgtctgc gctccttctc
     9241 gcaatcgttt tcagcgaacg attgtccgca ccgctgggag tattggcggc cggaacgcga
     9301 gccgttgcgc agggcgattt cagccagcgt catctggtca agtcgaacga cgaacttggc
     9361 tcgctcaccg aatccttcaa tatcatgaca atgcaattgg cggatgcgca ggccgacgcg
     9421 cagcgtaatc agcagcaact gaccgccgcg aaggcttacc ttgaaagcgt gctaggcaac
     9481 ttgtctgcgg gcgtaatgtc gttcgacgct gaatttcgtc tgcactcggc caaccgtagt
     9541 gcagagcaaa ttctcggcgt tagcgttggt gaattgattg gcagccatcc ggatgaatgg
     9601 gccaatcgtg agccgagttt agcggcggta ggcaacgcgc tgcgcgaggc actggtaaaa
     9661 agtcgagatg agacctggga ggcacaagtg gagttatccg gcccaactgg acaacaggtg
     9721 ctcctgttgc gtggctcgaa actgtctgcc gataccgagc ccggcatggt catgattttt
     9781 gatgacatta cacgaatgcg tcaggcccaa cgcttggccg catggggtga agttgcccag
     9841 cgtctcgcgc acgaaatcaa aaacccgctg acaccgatcc agctgtcggc cgagcgcttg
     9901 cagcatcgtc tggctgacaa gctatccagt caggaagcgg agatcttgca acggtcgacg
     9961 caaaccattg tgaaccaggt ggcagcattg aaggcaatgg tggatgcctt cagtgtgtat
    10021 gcgcgctcgc cggtgcccga cttgcagccg ctcgatctca atgtgctgac ccgcgaagta
    10081 ttgggcctgt acgagtcttc ccgtccggca ttgcaattcg agtttgctcc ctcgcttcct
    10141 ctcgtatctg gcgacaccgc caagctgagg caggtcattc ataatctgct tcagaatgca
    10201 gagcaggcgg tctccgaggt gccggtgccc cgcattgtcg tgcgaaccga agcgaccgat
    10261 gccggcgtcc aactcaccat gagagacaat ggaccgggat ttccggaaaa cgtaatcggg
    10321 cgtgtatttg aaccttacgt caccaccaaa tcaaagggaa cgggcctggg gttggccatc
    10381 gtcaagaaga ttgtggagga gcacaatggc agaattgtcg tcgctaataa cctttcgggc
    10441 ggggccagtg tgacgatcct gctgccggct gcaccgggga aaagtactgg aatgcgcctc
    10501 atcaggcagg ttgggatatg agccagattc tcgtcgtcga cgacgaagtt ggaatacggg
    10561 aactgttatc ggaaatcctg cgcgatgaag gccatcaggt aagactcgct cagaatgcgg
    10621 gcgaggcgcg catgattcgg gggcgtttgc gaccggacct ggttttgttg gacatctgga
    10681 tgccggatac cgacggcatt accctgctga aggagtgggc cagcaccgga caacttacca
    10741 tgccggtcgt catgatgtcg ggtcacggca ctatcgatac cgccgtagaa gcggcccgta
    10801 tcggcgcata tgcttttctg gaaaaaccga ttccccttca gaagctgttg aacaccgtcg
    10861 gcactgcact taagcaaggt tctattccgg tcaatgccac agccgtgccg gttactgtag
    10921 gcaaatcgaa ggcagtcgac caattgcgca cgcagctgga acggctccgt acattgaagt
    10981 catccgcatt gttcgtcggt gaaccggggg cggggatgga agaatgtgcg cggttgctgc
    11041 accaaaacaa cacgccctgg gtcgccccgg aagatttttt ggcgctggat cacttggaga
    11101 caaacctcgt gggtcaggcg gccgaggggg tactgttcct gcgtgaaatc ggacagctca
    11161 cgcgcctcca gcaaaagaac ctgctcaacc aaatgctgaa gctcgaccgc taccagaccc
    11221 ggctactgtc ttccagctca gtagacgttg ccgagcttgt ggcggccggt tcattcgagc
    11281 cgaggctata cacgttgttg gccggggtgg ttcttcgcgt gccgagtttg cgcgagcgtg
    11341 ctgaagatgt tccggaactc gccagcctgg tcctgtcgcg cgcgatcgag gcgcgtgagg
    11401 ttcctgcccg cagtttcaat accgccgcat tgaacacgtt gcgtaattac cattggcccg
    11461 gcaacctcct gcagctggaa agcgtggtaa agacagtagg gcaacttgcg tcggggccgg
    11521 agattggcgt ggctgacgta acgggtgcac ttgcaaaatc ggatgggccc cttccaagcc
    11581 cggtgcagga tttgccactt gacgtggaac ttcgtcaggc gcgcgatgct ttcgagcgcg
    11641 tttatttcga atatcacctg acccgggaac gcggcaacat gagccgcatt gcagaacggg
    11701 tcgggctgga acgcacgcac ctataccgaa aattgaagca actcggcatc aagctgccac
    11761 gtcgtgccga tgaatgatct gatcatcctc ggctaaacta gcgtcgcaat tttttccccc
    11821 gcgggatatt tcagttcttc aaaggaagat atggcaacca ggaacgaact tgccaacgct
    11881 atccgtgcgc tagcaatgga tgcggtgcaa aaggcaaatt ccggccatcc cggcatgccg
    11941 atgggcatgg ccgacatcgc cgaagtgcta tggaacagct atctgcgcca caatccggct
    12001 aaccctggtt gggcagatcg cgaccgtttt gtactgtcca acggccacgg atcaatgttg
    12061 ctgtatgccc tgctgcacct gaccggctat gccttgccaa tggaccagat caggcgtttc
    12121 cggcaattac attcgatgac gcccggacat cctgagctag ggataacccc cggcgtggaa
    12181 acgacgaccg gtccactcgg acagggtctc ggcaatgcta tcggcatggc gctcgccgag
    12241 cgactgctgg ccgcagaatt caaccgtccc ggattcgcga tcgtagatca tcgtacttat
    12301 gtcttccttg gagacggctg tctgatggaa gggatatcgc acgaggcatg ctccctggct
    12361 ggcacgcttg gactgggcaa attgatcgct ttctatgacg ataatggaat ctcgatcgat
    12421 ggccatgtcg acgggtggtt cacggacgaa acgccgaagc gtttcgaggc ctacggctgg
    12481 catgtcgtgc gcaatgtcga tggccataat gcggctgcga ttcaggccgc cattgaagcg
    12541 ggcaagaaga tactcgacaa gcccactctg ctttgctgca agacagtgat cggcaaaggc
    12601 gcaccgaaca aggcgggtag tcacgatagt cacggcgctg cgctcggaga aaaagaagtt
    12661 gcggcgaccc gcgcgaatat cggctggagt tatccagcat ttgaaattcc ggcagacata
    12721 tacgccggct gggacgcgcg gacaaaaggt gctgccagtg aagccgcctg gaatgccgga
    12781 ttcgattcgt atgcaaaaac gttcccgcac gaggcggcgg aattcaagcg ccgcatgtca
    12841 ggcgagttgc cagaaagctg gcgcgaacat gcagatcgct ttgtcgccaa cgttcaagcg
    12901 aaagctgaaa ccatcgccag ccgcaaagcg tcgcagaatg cgatcgaggc gctcgcaccc
    12961 gctctgccgg aattaatcgg cggttcggcc gatctgaccg gctcaaatct aacgctctgg
    13021 tctggggcca aggctgtgac gagaagtacc ggtggaaact acgtttacta cggcgtgcgc
    13081 gagtttggca tgtccgcaat catgaacggc ctgacactgc acggcggatt aatcccatac
    13141 ggaggcacat ttctcacatt ctcggactat tcgcgaaatg cgttgcgcat ggcggcgttg
    13201 atgaaagtcc ggaccttgtt cgtgtttacc cacgactcca tcggcctcgg ggaagatgga
    13261 ccaactcacc aatcggtcga gcacgcggca agcctgcggc tgattccaaa catggacgtc
    13321 tggcgccctt gcgattccgt ggaatccgcg gtggcctgga tcgcggccat agagcgaaag
    13381 gatggcccgt cctgtttact gttctcgcga cagaatctgc cttttcagat gcgcgacgcc
    13441 cgcgccatca accagattgc gcgaggcggc tacgtgctgt ccgattcagc caacgccaag
    13501 gcgatcatta ttgcgaccgg atcagaggtg gcccttgcag tggctgctca gaagcttctg
    13561 gcaacggatg gcatcggggt aaaagtggta tcaatgccga acacgaacgt gttcgatcgc
    13621 cagggtgcaa gttaccgcga gtccgtattg ccaaagaacc tcaggcgcgt cgcggtcgag
    13681 gccggcgtga cggactattg gcataaatac gtcgggctcg acggagaagt agtcggactg
    13741 gatcgattcg gcgaatcagc gccagctggt gaattgttca ggtttttcgg catcacggcg
    13801 gaagctgtgg ccgatgcggt gaaacgggtg atttgaacag cgcttcatcg cccttgagcg
    13861 ccaaaggtaa aggaccagat ggacaaggcg atgcggttgg ttgttgatat cggaggcact
    13921 aatgcgcgac tggccctggt gcccccggat tccttactgc cacagcagga aaaacgcgtg
    13981 cgctgcgccg atttcgatag cctggaacac gccgtgcagc actatcttca ggagtgcggc
    14041 gacccggcgg tgcgcgaagc ggcgctcgat gtggctaccg ggatcaccgg cgattttgtg
    14101 catttgacca acggtccgtg gggattttcc attgatcaga cccgtcgcgc cctgcgcctg
    14161 gataccctgc atgtggtcaa cgattttacg gcgctcgctc tggccattcc tctattgctt
    14221 ccgcatgaac ttcagcaaat cggtggcggc tccgcggttc ccgaaacggc gatcgcagtt
    14281 attggacccg gtaccgggct gggtgtgtcc ggactgattc cggtgggcgg tcgctggatt
    14341 ccgctacagg gagaaggtgg tcataccgcg ttcagtccca ttggcgacga agaaatcggt
    14401 gtcctgcgct ggctgcaaca gcgatacaac catgtatcca cagaacgcgt agtgagcggc
    14461 atgggactgg aaaatttgta ccaggcgttg tgcgcgatgg ccggcgttca ggcgaaacct
    14521 ctgagtcccg acaagattac cgcatcggcc ttgtcgaata ccgatgtcca gtgcaaggca
    14581 gcgctccaga tgttctgtgc cattctgggc actgcggctg caaaccttgt tgtaacactc
    14641 ggtgcccgag ccggctgtta tatcggcggg ggaattgttc cgcgtctggg agcctatttt
    14701 gcacactcgg cgtttcggtc gcgctttgaa agcaaggggc gattttcaag ctacgttgct
    14761 gcggtcccga cgtacgttat tcttggcgag acgccagcgc tgcgcggact tgccacgctg
    14821 ttttagaact caaattgaaa ccatggatgg actgataact ggtattgggc tgctggctgg
    14881 tttgttgacg actgcggcat tcatcccgca agtctggaaa atctaccaaa caaaatctgg
    14941 caaagacatt tccggaagaa tgttttggtt attcagcatt ggcatcgcgc tctggctggt
    15001 ctacgggatg ctgctaagct ctatccccct cattgcttcc aacgtggtaa cgctcgtcct
    15061 gtccctaacc attctggcgc ttaaataccg gtattcgcgc gccacctgat aaccgggcgg
    15121 tccggacaac tgactcgctg tttggtccgc gctcaggatg gcgcgcgcgg gcctccatga
    15181 cattgtgctg aacgacgctg cactaatgcg atccaaggtt ccagtcagca gtcgcctgcc
    15241 tcagcacggt gccagcactg accacggctg tgacggttta gccgactgca gtaaaatccc
    15301 gttttgaacg gagattctta atgaccattc gggttgcaat aaacggctat ggccgtatcg
    15361 gccgaaacat cctgcgagca cattacgaga acggcaagaa ataccctata gagattgtcg
    15421 cgatcaatga tctcggcgat gcaaacatta acgcccacct caccaagtac gatactgccc
    15481 atggcaaatt tccgggcacc gtagcggttg acgggaataa tctggttgtc aatggcgacc
    15541 aaatcaaagt ttgcgcccag cgcagtcccg cggatttgcc gtggcaagat ttgcgcgttg
    15601 acgtagtgct ggaatgcacc ggattgttta ccagtaaggc aaaggccggt gctcacctga
    15661 cggcgggcgc gaagaaagtc atcatctcag cgccgggcga caaggacgtt gatgcaacag
    15721 tcgtgtatgg agtcaatcac ggtacgttga ggcctgccca tacggtgatc tccaacgcaa
    15781 gctgcacgac caattgcctt gctccgctgg tgaaggcgtt gaacgaccgg atcggcgtgg
    15841 taaatggatt gatgaccacg atccacgcct ataccaacga tcaggtgctg accgatgtct
    15901 accacactga tttgcgccgc gcgcgttcag caaccatgtc catgattcca accaaaaccg
    15961 gagccgccgc ggcggtcggc ctggtgttgc cggaactgaa cggcaaactg gatggcttcg
    16021 ccatgcgcgt gcccaccatc aacgtgtcga tcgttgatct gacgttcgtt gcgaaacggg
    16081 cgaccagcgt ggaagaggtg aactctgcgg taaagcaggc gtcggaagcg gaactgaagg
    16141 gcattcttga gtacaacaag gatccgctgg tctctgtgga tttcaaccac aatcccgcct
    16201 cctccgtatt cgattcgacg ctcaccaaag tctcggtggg aacattggtc aaagtgtgtt
    16261 cctggtacga caacgaatgg ggattcagca accgcatgct cgacactacg gtggcgttcg
    16321 taaacgcgaa ataagaacgt tttccctggg gtgcaaaccg gttgcttcag cccctttggg
    16381 ttactccacc aactgtgtac ggattgatct gacatcatga ccattctgcg gatgaccgat
    16441 ctgaatttgc gcggcaagcg cgtattcatt cgtgccgact tgaacgttcc gcaggataag
    16501 tccggatata ttactgacga cacgcgcata cgggcgtccc tgccggcgat ccggatggcg
    16561 cttgacgccg gtgctgcggt gatggtgacg tcgcacctcg gtcgtccggt agaaggccaa
    16621 ttcaagcccg aagattcact ggcccctgtg gcacaacgcc tttcccagca gctcggcgtg
    16681 ccggtgcctc taaaggcgaa ctgggtaaac ggggtggaaa tagagcccgg gcacatcgtg
    16741 ttgctggaaa actgccgctg taacaagggt gagaaaaaag acgatgacgg gctcgcacga
    16801 aaaatggcgg cgctgtgtga tgtctacgtc aatgatgctt ttggcacagc tcatcgggcg
    16861 gaggcaacga cacatggcat agccagatat gcggcggttg cttgtgccgg accgctcatg
    16921 gcggctgaac tcgatgcact gggcaaggcg ctgcgccagc caaaacgtcc gctgatagcg
    16981 atcgttgccg gttccaaggt gtcgaccaag ctgaccatcc tggctgaact cgcgaaaaaa
    17041 gtggaccaac tcatcgtggg cggcggcata gccaatacct tcatgcttgc ggccggtctg
    17101 tcgatcggca aatccctggc agaaccggac ctggccggtg aagcccgcag catcatgcag
    17161 accatcaaat ccaaaggcgg ggccgtgccg attccagtcg atgtggtgtg cgccaaggaa
    17221 ttctcggccg aagcggcggc cacgataaaa gacgcccaac aagtcgccgc agacgatctg
    17281 attctggata tcggccccag gacagccgcg caactggcgg ccttactggg tcgggccggc
    17341 acgatagtct ggaatggccc ggttggtgtt tttgaattcg agcgctttgg tgaggggacc
    17401 agagtgcttg cacaagcgat cgccgattcg cccgcatttt ccattgccgg aggcggagat
    17461 accgtcgccg cgattgctaa atacggaatt ggagaccgga ttggctatat ctctaccggc
    17521 ggtggcgcgt ttctcgaatt tctcgagggc aagaagcttc ctgcggtcga aatactcgag
    17581 tcccgcgctg ctgcacaacc acaggaaagg gcatgacaca aaagagcacg aaaatcgtag
    17641 cgaccctcgg tccggcgtcg agtgaccggg acgtactgat gcgcatgatt gcgcatggcg
    17701 tcgatgtggt tcgcatgaat ttttcccatg gcaccaagga agagcacctc gctcgcgcgg
    17761 aactggtgcg cgacgtcgca cgctccctgg agcgcacagt ggggatcatg tgcgatctgc
    17821 aggggcccaa gatccggatc gggaaattcg aagccggcag agtcacgctg aacaagggag
    17881 aaacctttac gctggatgcc gactgcccgg tgggcgacca gcatcaggtt ggtctcgact
    17941 acaaggaatt gccgcatgat gttggcgcgg gcgcggtgct gctcctcgac gacgggcgtt
    18001 tggtgctgga tgtgcaaaaa gtgcaggaca gccgcattac cactacggtt cgcgttggcg
    18061 gcgtattgtc gaataacaaa ggcatcaacc gtcagggtgg cgggttaacg gcgccagcgc
    18121 taaccgcaaa ggacatggaa gacatcaaga ccgctgtcgc catgcgcgcg gactttctcg
    18181 cggtttcgtt tcccaagag
//
LOCUS       DATMZQ010000509         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4823655, whole genome shotgun sequence.
ACCESSION   DATMZQ010000509 DATMZQ010000000
VERSION     DATMZQ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4823655"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VN496_16280"
     CDS             <1..435
                     /locus_tag="VN496_16280"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HXF18565.1"
                     /translation="KSVHPDEPGLVFDRRRRVLEVCWTGDVGRSFHQTVADALAALNP
                     FSEEAAAFEVSYYLEDGKDEFSLVFVGPTQDAILEAQRALMLEDVRSLLSRQFSDPEI
                     AQVVALVNQLFERNVKAGTSNTQTSTSPSEPMPMPAGRKHLH"
     gene            574..2001
                     /locus_tag="VN496_16285"
     CDS             574..2001
                     /locus_tag="VN496_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXF18566.1"
                     /translation="MPDLAQPSPRLPAVKLDARGRRRVDLRLVITLALPLFVNSGIQA
                     LLNLTDTWFIGRISTDATAAVGASYWFVIVAILFFGGPGLAVQTLAAQAFGAGDNHSA
                     SRAAWAGVRGMLLVVPAFAIIAVLGPTLMGVLKIEAQVAQLAADYWFPRLLGGAFAVG
                     IWATTGFFNGIGRTGVTLALMTFVAVLNAVLNQLFIFELGWGIAGSGWATTAALGVGL
                     LVSLALLVRRTIDAEFASRTTWRDSRAELRTLFRVGFPIGLFPAIDVAGLAAFQAMQV
                     AAGPVDGAATQIVMMLTSIAYLPTMGFALAGTTLVGQSIGAGDKDWATKVGNTTIKLA
                     VGYMGLVTIFLAVIGPWLIPLFVNAADPHASNVIALGRTLLWFAAGYQIFDGLNLGAG
                     FCLRGAGDMRVPTLTIVFLSWFGFVPFTHMFTFAPGHGWVEFLPQFGYGAIGGWAVAL
                     VYCVVLGSFIYLRWRSGAWRKIKLR"
     gene            2065..3333
                     /locus_tag="VN496_16290"
     CDS             2065..3333
                     /locus_tag="VN496_16290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF18567.1"
                     /translation="MQTMLARLCCLAALLCTATLLPAAPLAQSSPEEVGLSAERLGRL
                     DSALQKAVDSGELPGAVVFIARDGQLVYAKSFGWLDREKKIPMNNDAIFRLYSMTKPV
                     VSVAAMMLVEEGKLGLQEPVSKYIPEFKEMKVGVESKDAEGKPALTLVEAKRPITVQD
                     LLRHTSGLTYGVLGAPTAVKKLYNDANIFSQKWVLADFCRALAKLPLQFQPGTTWEYG
                     HSTDVLGRVVEVVSGQTLDAFLAKHIFEPLKMPDTGFQVPAAKRARIAQPMPDVYTGK
                     TPELIDFGKPQTFFAGGHGLVSTAGDYLRFAQMLANGGELDGVRLLGPRTVEYMTSNH
                     VNPQIDKGLTYLPGPGYGFGLGFATRIDRGQSEWPGSPGDFYWGGYAGTYFWVDPAEE
                     LVPVFMTQEPARRQYYRVQLRNLVYQSIVK"
     gene            3351..>3619
                     /locus_tag="VN496_16295"
     CDS             3351..>3619
                     /locus_tag="VN496_16295"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXF18568.1"
                     /translation="MRLRFLLLLTVIACSASSFAHAQVESQRAFLLVAVPGMLDPNFS
                     ETVVLAVRGDDGGPLGLILNRPSTVELRSLYPDRTELAGRRDVVFL"
BASE COUNT          663 a         1044 c         1106 g          806 t
ORIGIN      
        1 aaaagcgttc atcctgatga gcccggactg gtattcgatc gtcgtcgtcg cgtcctggaa
       61 gtatgctgga ccggtgacgt cggccgcagt ttccatcaga ctgtcgctga tgcgctggca
      121 gcgctgaacc cgttttccga agaagccgcc gcttttgaag tcagctatta cctcgaagat
      181 ggcaaggacg agttcagcct ggtattcgta ggtccgacgc aggatgcaat tctggaggca
      241 cagcgcgcgc tcatgctgga agacgtgcgc agtctgctgt cgcgccagtt cagcgatccg
      301 gaaatcgcgc aggtcgttgc actggtcaac cagctgttcg agcgcaacgt caaggccggg
      361 acatcaaaca cgcaaacgtc caccagtcca tcggaaccca tgcccatgcc ggcgggacgc
      421 aaacacctgc actagctgac tgcatgtccg cgtgacgcgc gacagccgac aatcctgtct
      481 tgcttcgaac tgtacaatgc cgtgaacgcc tgaaaccaat ttcccaaact gcgcgcccgg
      541 ggaacagccc gggcgctttt tttgtaagca atcatgccgg atctggcgca accttccccg
      601 cgactgcccg ctgtcaaact cgatgcacgc ggccggcgtc gcgtcgactt gcgcctggtg
      661 attacgctgg cattaccgct gttcgtcaac agcggcatcc aggcgctgct gaatctcacg
      721 gacacgtggt tcatcgggcg catttcgacc gatgccacgg cagcggtcgg cgcttcgtac
      781 tggttcgtaa ttgttgcgat cctgtttttc ggcggtcccg gacttgcggt gcaaaccctg
      841 gcggcacagg cattcggcgc cggggacaat cattcggcat cgcgcgcggc atgggctggc
      901 gtgcgcggca tgctgttggt agtgccggcc tttgcaataa tcgctgtgct tggaccgact
      961 ttgatgggtg tgctgaagat cgaagcacag gttgcgcagc ttgcagccga ttattggttt
     1021 ccgcgactgc tcggcggcgc ctttgctgtc ggcatctggg ccaccacagg gttcttcaac
     1081 ggcataggcc gtaccggcgt cacgcttgca ctgatgactt tcgttgccgt gctgaacgcc
     1141 gtactgaatc agctgttcat ctttgaactg ggctggggca tcgccggaag cgggtgggcc
     1201 acgaccgctg cgcttggcgt aggcttgctc gtttcccttg cgttgctggt gaggcgcaca
     1261 attgatgcgg aatttgcttc gcgcaccacc tggcgcgaca gtcgcgccga gttgcgcacg
     1321 ctgtttcgcg tcggctttcc gatcgggctg tttccggcca tcgacgtggc cggacttgcc
     1381 gccttccagg cgatgcaagt tgcagccgga ccagtggacg gcgcggccac acagatcgtg
     1441 atgatgctga cttcgattgc atatctgccg accatggggt tcgcgctcgc aggcaccaca
     1501 ctggtgggcc agtccatcgg cgccggggac aaggattggg ccactaaagt gggcaacacg
     1561 acgatcaaat tggcagtggg ctatatgggt ctggtcacca tattccttgc tgtcatcggt
     1621 ccctggctca ttccattgtt cgtgaacgcg gccgatccac atgccagtaa cgtgatcgcg
     1681 cttgggcgca cgctgttgtg gttcgccgcg ggttaccaga ttttcgatgg cctgaacctc
     1741 ggcgcagggt tttgtttgcg cggcgccggg gacatgcgcg tgccaaccct gacgattgtt
     1801 tttctatcct ggttcgggtt tgtccctttc acgcatatgt tcacttttgc gcctggacac
     1861 ggttgggtcg aatttctgcc gcagtttggc tatggtgcga tcggtggctg ggccgttgcg
     1921 ctggtgtatt gcgttgtgct tggcagcttc atctatctgc gctggcgctc cggggcctgg
     1981 cgcaagatca agctgcgcta agatagatac tgaactacac catcgcgcag gtcataaagc
     2041 gcacctctat tcgggagaag attcatgcaa accatgctgg cgcgcctttg ctgccttgcc
     2101 gccctgctgt gcacggcgac tctgttgcca gctgcacccc tggctcaatc cagtcccgaa
     2161 gaagtgggct tgtccgcgga gagactcggc cgcctcgatt cagccctgca gaaagcagtt
     2221 gacagcggcg agctaccggg cgcggtggtg ttcatcgccc gcgatggaca gctggtgtat
     2281 gcgaaaagtt ttggctggct ggatcgggaa aagaaaattc cgatgaacaa tgatgcgatc
     2341 ttccgccttt actccatgac gaaaccggtg gtgagtgtcg ccgccatgat gctggtcgag
     2401 gaaggaaagc tgggattgca ggagccggtt tcgaaataca tacccgagtt caaggaaatg
     2461 aaggtcggcg tggagtcgaa ggatgccgaa ggcaaaccgg cactgacgct ggtggaagcg
     2521 aaaaggccga tcacggtgca ggacctgctg cggcatacct cgggcctgac ttacggcgtg
     2581 ctcggcgcac cgactgccgt gaagaagcta tataacgatg cgaatatttt ttcccagaaa
     2641 tgggtgcttg cggatttctg ccgggcgctg gcgaagttgc cgcttcaatt ccagcccggc
     2701 acgacttggg aatatggaca ttccacggat gtgctcggcc gcgtcgtgga agtggtttcg
     2761 gggcaaacgc tggacgcttt cctcgccaaa cacattttcg aaccgctgaa gatgcccgat
     2821 accgggtttc aggtgcctgc cgcaaagcga gcccggatcg cgcaaccgat gccggatgtg
     2881 tataccggga aaacacctga actgatcgat ttcggcaaac cgcaaacatt ttttgccggt
     2941 ggtcacggac tggtctccac tgccggcgac tacctgcgtt tcgcccagat gctggccaat
     3001 ggtggcgaac tggacggcgt gcgcctgctc gggccgcgca cggttgaata catgacgtcg
     3061 aaccacgtca atccacagat cgacaaaggt ctcacttacc tgcccgggcc cggttacggc
     3121 tttggcctcg gctttgccac gcgcatcgat cgcggccagt cggaatggcc cggttctccc
     3181 ggcgattttt attggggagg ctatgcaggc acttatttct gggtggatcc cgcggaagaa
     3241 ctggtcccgg tattcatgac gcaggaaccg gcgcggcgcc agtattaccg cgtccagctg
     3301 cgcaatctcg tgtaccagtc gatcgtgaag taaattgccg ctcttgtgac atgcgtctcc
     3361 ggttcctgct gctgctaacc gtaatcgcat gctcggcgtc ttcatttgcg cacgcccagg
     3421 tcgaaagcca acgcgcattc ctgttggtgg cagtgcctgg catgctcgat ccaaacttca
     3481 gcgagacagt cgtgctggcc gtgaggggcg acgacggcgg tcccctggga ttgatcttga
     3541 atcgacccag tacggttgaa ctgcgctcac tgtatcccga ccgcaccgaa ctcgcgggac
     3601 ggcgtgacgt ggtgtttct
//
LOCUS       DATMZQ010000510         2919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4475965, whole genome shotgun sequence.
ACCESSION   DATMZQ010000510 DATMZQ010000000
VERSION     DATMZQ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4475965"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VN496_16300"
     CDS             complement(<1..379)
                     /locus_tag="VN496_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18569.1"
                     /translation="MATVTFSGFAVNTFAPSYGSEVLEAAMVDFLAGRFTVSLTPPPP
                     SAPTLVEISTFGPGPFLLIQIHGSFDIASEAAVLNSFLTSVHLQFGASNIVDFSGMHQ
                     TLNSFLSVGLTQGVFAGNDTITGS"
     gene            complement(839..1168)
                     /locus_tag="VN496_16305"
     CDS             complement(839..1168)
                     /locus_tag="VN496_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18570.1"
                     /translation="MIEISQSVTGNRASGMLPLFISRLTLVLTASAAFVPLRASAHLL
                     DGSPDPLPRWVPIVALIIVGIMGGTVGFAISKHRASMEQQKKQKLQRAAEAVASPAKE
                     QPPAKPQ"
     gene            1277..2749
                     /locus_tag="VN496_16310"
     CDS             1277..2749
                     /locus_tag="VN496_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18571.1"
                     /translation="MFSISIAAFADEDAHSLTIYSTMTPGSISPDQYRNGGGGHSLPG
                     YATVRHERDMVFKAGRNSVRFIDVAGLIDPTSVSFSSLTDPIGTRVMEQNFQFDLINP
                     EKLLTRYIDREIAVERMVGNQIETLEGKLLSTQGGMVIREGDGSVRTLPYNVGVKLPS
                     LPGGLITRPTLAWDVIAGKAGTHKARVAYQTAGMTWWADYNLTFAEGKDANSCKVDIG
                     AWVSILNQSGGSYPDAKIKLVAGDVHRAEYLAANAPMMAKRAMADAAEEKANFVEKSF
                     FEYHLYTLGRPSSLPDNSTKQIELFPKVAGVPCDKTLVYYGQAGQWYGGALMTDRNYG
                     TTSNKKVDTYLSFRNRSEDGMGVPLPSGRVRVAKLDTADGSLEFIGEDRIDHTPKNET
                     VQLKLGSAFDVVGERRQVDFKIDSNRNTMTEEIEVKIRNRKDEAVRVVVKENLYRWTN
                     WKITQANQTYEKQDSRTIHFPVNVAADGETVVRYTVRYTW"
BASE COUNT          687 a          825 c          826 g          581 t
ORIGIN      
        1 cgctgccggt gatcgtgtcg ttgccggcaa acaccccctg cgtcaggccc acggacagga
       61 aggaattgag ggtctgatgc atgcccgaaa aatccacgat attgctggct ccgaattgga
      121 ggtggacact ggtcaggaaa ctgttcaaca ccgccgcctc ggacgcgatg tcgaacgacc
      181 cgtggatctg tatcagcaag aacggaccgg gtccaaacgt ggaaatctcg accaaggtcg
      241 gcgccgaagg cggcggaggc gtcagggaaa cggtaaagcg cccggccagg aaatcaacca
      301 tggccgcttc aagtacctcc gagccatagg agggcgcgaa ggtattaacc gcgaacccgc
      361 tgaacgtaac cgtggccata gtgaataccg attcctgtct ggactgaagt tattgtcagg
      421 agtcggtcag gggaggtgga ccaagcacaa tgacaaatgc aagcgcgcac actctctcat
      481 actgcgacgc tccggtcaaa aacagctcaa ccggcgtgga tggtcgctac tcgtcgcgtg
      541 gcaatcgata tggaatgaat gtccggcctt cgtgagcgtt ctgaaaactt gcgcccatgc
      601 agcagcaacg cgatgcggcc attcctagca cctggaggga tccatgtaag ccattttcat
      661 ggggtcacct tccatggtta atggcagaca gtggacctcg aaggggtaag gaaattgttc
      721 cagtccgatg ccgaaacatt gtgggtcgac atgcgctacc acggcgcaga acaggaatac
      781 ttccgccatc cacctaatga cgcaagtcag agtactggag tgctgtccag tttcgttttc
      841 actgcggttt agcgggcggc tgctccttcg ctggcgaagc aaccgcttcc gcggcgcgct
      901 gcagtttctg cttcttttgc tgctccatcg atgcacgatg tttgctgatg gcgaatccaa
      961 ccgtaccccc catgatcccg acaatgatca acgcgacaat cggcacccag cgtggcaacg
     1021 ggtcgggaga gccgtccagc agatgagccg acgcgcgcaa cggcacaaaa gcagcgctgg
     1081 cagtcaagac gagtgtcagc ctgcttataa ataacggcag catgcccgac gctcggtttc
     1141 ccgttactga ctgcgatatt tcgatcactg tttaaccccg tttccggaac aagcccgttt
     1201 acagatacgt aattcaattc accggaaagg aatatccgtg tttgcacacg ctctgcgctc
     1261 cccattagcc ctactgatgt ttagcatatc gattgcggct ttcgccgacg aagatgccca
     1321 ctctttgacc atctatagca ccatgacacc cggttcgata tcgcccgatc aatatcgaaa
     1381 cggcggaggc ggccacagtc tacccggcta tgcaaccgtg cgtcacgaac gggacatggt
     1441 gttcaaggcc ggacgcaatt cggtgcgctt catcgatgtt gctggcctga ttgatccgac
     1501 cagcgtgtcg ttttcctcgc tcacggaccc gatcggtacg cgtgtcatgg agcaaaattt
     1561 ccagttcgat ctcatcaatc cggaaaagtt gttgacgcgc tatatcgacc gcgaaattgc
     1621 ggtcgagcgc atggtgggca accagattga gacactggaa ggaaaactgc tgtccacaca
     1681 aggcggcatg gtgatccgcg agggagacgg gtcggtccgc acgctgcctt acaacgttgg
     1741 tgtaaaactg ccatcgcttc cgggcggact cataacgcgg cccaccctgg cgtgggacgt
     1801 cattgccggc aaagctggaa cccacaaagc gcgcgtggcc taccagactg ccggaatgac
     1861 atggtgggcg gattacaacc tgacttttgc cgagggcaag gatgccaact cttgcaaggt
     1921 ggacatcggc gcctgggtca gcatcctgaa ccaatcgggt ggcagctatc cggacgcgaa
     1981 aataaagctc gtggccggcg acgtgcatcg tgccgagtac ctggccgcga acgcgccgat
     2041 gatggcgaaa cgggccatgg cggatgccgc ggaagaaaag gcgaacttcg ttgagaaatc
     2101 gttcttcgaa tatcacttgt acacgcttgg gcgcccaagc tcgctgccgg acaattccac
     2161 caagcagatt gaactgtttc cgaaagtggc tggcgtacca tgcgacaaga cgctggtgta
     2221 ctacggccaa gccgggcagt ggtatggcgg tgcgctcatg accgaccgca actacggcac
     2281 cacgagcaac aagaaagtcg atacctatct gtcgttcagg aatcgctctg aggacggcat
     2341 gggagtgccc ctgccgtccg ggcgagtgcg cgtcgccaaa ctcgacactg cggacggcag
     2401 tctcgaattt attggggaag atcgcatcga tcacacgccg aagaacgaaa cagtgcaact
     2461 caaactcggg agcgcgtttg acgtcgtcgg cgagcgtcgt caggtcgatt ttaaaatcga
     2521 ctccaaccgc aacaccatga ctgaagagat tgaagtcaag atccgcaacc gcaaggacga
     2581 agcggttcgt gtggtcgtga aggagaacct gtaccgctgg accaactgga aaattaccca
     2641 ggccaaccag acctacgaaa agcaggattc ccgcaccatc cactttccgg tcaatgttgc
     2701 ggccgatggc gaaactgtag tgcggtacac cgtgagatat acctggtagg gcgcacggct
     2761 aacacgctgc gctcttgcgc aaggccgggt gatgtgtaga tctgcgtctt acattactcg
     2821 gccttgatcc ctgcctcctt tacaatcttc gaccatttcg tcctttgtgc ggcaaggaac
     2881 agagcaaatt gttccgaggt acttcccgca gtttcaacc
//
LOCUS       DATMZQ010000511         3526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4827857, whole genome shotgun sequence.
ACCESSION   DATMZQ010000511 DATMZQ010000000
VERSION     DATMZQ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4827857"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VN496_16315"
     CDS             complement(<1..1029)
                     /locus_tag="VN496_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXF18572.1"
                     /translation="MNALFDCTHLNGVLAVAVAWLVIGVAGVCAMRRVELVPRILLPA
                     GSVFGVALFAIAAHALFAPAEAAVLPIGLPGLPFHFRLDGLSAFFLMVIGGAAAGVSA
                     FAAGYLRKVEGEMPPGLVGLQYHVFLASMVLVVLADDAYAFMVMWETMALSSFFLVIV
                     NHRIEENRRAGYLYLLIAHIGAIGILLCFGVLQANTGDYTFANMRVQEHTPFWASMAF
                     LLALFGFGAKAGLLPLHVWLPEAHPAAPSPVSALMSGVMLKTAIYGFLRITFDLLHVH
                     LWWWGLLLLGIGLITGLFGIVFAAVQVDMKRLLAYSSIENIGLIFAAIGLSVLFSSFG
                     MTPMAALAL"
     gene            complement(1128..1319)
                     /locus_tag="VN496_16320"
     CDS             complement(1128..1319)
                     /locus_tag="VN496_16320"
                     /inference="COORDINATES: protein motif:HMM:NF039406.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HXF18573.1"
                     /translation="MENRTARLTILIDPRKKKIFEEICASHDVTSSQVVRKLIRQYII
                     ENAGERKLPDWLKSKDTAR"
     gene            complement(1540..2283)
                     /locus_tag="VN496_16325"
     CDS             complement(1540..2283)
                     /locus_tag="VN496_16325"
                     /inference="COORDINATES: protein motif:HMM:NF006118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18574.1"
                     /translation="MNTQRTLKIKDAVAVVTGANRGIGLAFVRELLERGARKIYAAGR
                     DPKGLDAIVALDRVRVVALRLDLLDQKQITEAAATTGAIDLLVNNAGVAAFGSILNGS
                     PELVARDMQTNYFGTLNVIRAFAPQLEKSGGGAIVNLLSVVSLANMPALGGYSASKAA
                     SWSMTQAVRAELAKKNISVHAVFPGPVDTDMAKDIQLPKTSPRDVARAVLDGVESGQD
                     DITPDSMSRDVHGAWLKDPKGVERQFGAM"
     gene            2379..3371
                     /locus_tag="VN496_16330"
     CDS             2379..3371
                     /locus_tag="VN496_16330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXF18575.1"
                     /translation="MRKAHEPITGCVGRRLGKLRARYHLLMDTETLETFVEVMRRGSF
                     AAVARDRGTDPSSISRQIAGLEQELGLRLFQRNTRRLAPTEAGAIYFARIEPLLDKVE
                     EARERAADVASEPKGVLRVTASVSFGQRCIVPVLPEFARLYQTLTVELALTDSMLDLV
                     AERLDVAIRLGPLEDESLVAQRLLAARYFVCASPAYLAQHDAIRTPHDIRNHECLLFA
                     LPRLRTRWTYRDKKGELTEIPVRGRILISTGLALHECALAGMGLALLPHWVIGKDLHH
                     GRLVDVFPEFEVTPTTFNTAAWFVYPSTTRPPKKVRLFVDYLQKEIRDNPRWMR"
     gene            3424..>3526
                     /locus_tag="VN496_16335"
     CDS             3424..>3526
                     /locus_tag="VN496_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXF18576.1"
                     /translation="MTVSESSKGVCLVVGAGDATGGAIARRFAREGFT"
BASE COUNT          773 a         1054 c         1023 g          676 t
ORIGIN      
        1 caaagcgagc gcggccatcg gcgtcatgcc gaaagaggag aacaggacgg acaggccaat
       61 cgcggcaaag atcagtccga tgttttcaat cgaggagtag gcaagcagcc ttttcatgtc
      121 cacctgcacg gcggcgaaga caatgccgaa cagtcccgta atcaggccga ttcccaacag
      181 cagcaagccc caccaccaga gatgcacgtg cagaagatcg aacgtaatgc gcaaaaatcc
      241 ataaatcgcg gtcttcagca tcacgccgct catcagcgcc gataccggcg atggtgccgc
      301 gggatgagcc tcgggcaacc atacatgcag gggcagcaag cccgccttcg caccaaatcc
      361 gaacagcgcg agcaggaatg ccatcgaagc ccagaacggc gtatgttcct ggactcgcat
      421 gttcgcgaac gtgtagtccc ccgtgtttgc ctgcagtacg ccaaaacaaa gcaggatgcc
      481 gatggcaccg atgtgcgcga tcagcagata cagatatcca gcgcgccggt tctcctcgat
      541 gcgatgattc acgatcacga ggaagaaaga agacaaggcc atcgtctccc acatcaccat
      601 gaaggcatac gcgtcatcgg cgagcacgac gagcaccatg ctggccagga acacgtgata
      661 ttgaaggccg accaggcccg gcggcatttc accttcgacc ttgcgtaaat acccggcagc
      721 gaaggccgac acgccggcag cggcgccgcc gatgaccatc aggaagaatg cgctcaggcc
      781 atcgaggcgg aaatggaacg gcaggccagg cagcccaatc ggcagcaccg cggcttccgc
      841 gggcgcgaac agcgcgtgcg cggcgatcgc gaacagcgcg acgccgaaca cgctgccggc
      901 gggcaacagg attctcggga cgagttcaac ccgacgcatt gcgcacacgc cggccacacc
      961 gatgaccagc cacgcaacag cgaccgccag aacaccgttc aggtgagtgc aatcaaataa
     1021 ggcgttcatc tttttccgcc ttctgcatca gggggactgc ggcaagggtg gctgcaaaaa
     1081 agtgggcacg ccgtgaattt tgactcggta atcaaaggca aggaccgcta gcgggcggtg
     1141 tccttgctct ttagccagtc aggaagtttt cgctccccag cgttttcgat gatgtactgc
     1201 cggatcagct tgcgtacgac ttgcgaagag gtgacatcgt gactcgcaca aatttcttcg
     1261 aagatttttt tcttgcgcgg atcgatgagg atggtgaggc gtgcggttct gttttccatg
     1321 gcagctctct gatgggctgc cagcagatta acatatcagt cacatatatg tattgtttga
     1381 ttataacttg attaaccgcg gagaacgcgc gagaacggaa aaaggcgcaa cccacgcgca
     1441 ggattgcccg ccatggtgcc gaaatggcga gcgcaaaaaa cataaagccg gagcggcatg
     1501 agggccggcc cggccttgcg ccagacttat gcagctcttt tacattgccc cgaactgacg
     1561 ctcgactcct ttgggatcct tcagccatgc gccgtgaacg tcacgcgaca tggaatcggg
     1621 agtgatgtcg tcctgcccgg attccacacc gtcgagcacc gcgcgcgcga catcgcgtgg
     1681 cgatgtcttc ggaagctgga tatccttcgc catatcggtg tcgacggggc ccggaaatac
     1741 cgcatggacg ctgatgttct ttttcgccag ttccgcgcgc acggcttgcg tcatggacca
     1801 tgaggcagcc ttggaagccg aatacccgcc gagcgccggc atgtttgcca gggaaaccac
     1861 gctgagcaga ttgacgatgg caccgccgcc agatttctcg agctgcgggg caaaggcccg
     1921 gataacgttg agtgtgccga aataattggt ctgcatatcg cgcgcgacca gttcaggcga
     1981 tccgttgagg atgctgccga acgcggcaac gcccgcgttg ttcaccagca gatcgatggc
     2041 gcccgtcgtg gcggccgcct ccgtgatctg cttctgatcc agcagatcca gtcgcagcgc
     2101 caccacccgg acgcgatcca acgcaacgat ggcatccagg cctttcgggt cccggccagc
     2161 cgcataaatc ttgcgcgcgc cgcgttcgag caattccctg acaaaagcca atccgatgcc
     2221 acgattggca ccggtgacca cggcaactgc gtctttgatt ttcagtgtcc gctgcgtgtt
     2281 cattcaaatt cctttcatta tgggtttcag gtttgaaaca tgcgcggact atagcgagcg
     2341 cccccggccg ccgaaagaca acatttggca caacactcat gcgaaaagcg catgaaccga
     2401 tcactggctg cgttggtcgg cgcttgggga aactccgcgc gcggtatcat ttgctcatgg
     2461 acaccgagac gctcgagacc tttgtcgagg tcatgcggcg aggcagcttc gcggccgtcg
     2521 cgcgcgaccg cggcacggat ccatcttcaa tatcccggca aattgccggg ctcgagcaag
     2581 aactcgggct tcgcctcttt cagcgaaaca cgcgccgttt ggctccaacc gaggccggag
     2641 ccatttattt cgctcgcatt gaaccgctgc tggacaaagt cgaagaggcg cgcgagcgcg
     2701 ccgcggatgt ggccagcgag ccaaaaggcg tgctccgggt aactgcatcg gtttccttcg
     2761 gacagcgctg catcgttccg gtgctgcccg agttcgcccg cttgtatcaa accttgacag
     2821 tcgaactggc gcttaccgat tcgatgctcg atctggtagc agagcgactc gatgtggcga
     2881 tccggctcgg accgctggag gacgaaagcc tggttgcgca gcgcctgttg gccgcccgat
     2941 attttgtttg cgccagcccg gcttatcttg ctcaacatga tgctatccgc acaccgcacg
     3001 atatccgcaa tcacgagtgc ctgctttttg cattgccgag attgcgcacg cgctggacat
     3061 atcgcgacaa gaaaggagaa ctgacagaga tccccgtccg gggccgcatc ctcatttcaa
     3121 ccggccttgc gttgcacgaa tgcgcgctcg caggaatggg gctggcgctg ctgccgcact
     3181 gggtcatcgg caaggacctt catcatggcc ggctagtgga cgtgtttccc gagttcgaag
     3241 tcactcccac gactttcaat actgctgcct ggttcgttta tccgtccacg acccgtccgc
     3301 cgaagaaagt gcgtcttttt gttgactatc tccagaaaga aatacgcgat aacccgcgtt
     3361 ggatgcggta ggactacgca tcctcgatta gactttggct cacaaggacg gaaccaataa
     3421 atcatgactg tttccgaatc gagcaaaggg gtatgcctcg ttgtcggcgc gggtgacgcg
     3481 acgggcggcg ccattgcgcg gcgtttcgcc cgggagggat ttacgg
//
LOCUS       DATMZQ010000512         3447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2320245, whole genome shotgun sequence.
ACCESSION   DATMZQ010000512 DATMZQ010000000
VERSION     DATMZQ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320245"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..452
                     /locus_tag="VN496_16340"
     CDS             261..452
                     /locus_tag="VN496_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18577.1"
                     /translation="MEKTNGIFDNRATMQREAWMDGEIIGVWPAAKCGTGLDTLMPWE
                     REALEARWGAYPNPPPRDT"
     gene            complement(524..2686)
                     /locus_tag="VN496_16345"
     CDS             complement(524..2686)
                     /locus_tag="VN496_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HXF18578.1"
                     /translation="MTDAARLDDGQNHVNALPPDDRTRVLKHGETFGVFDRYGDVRFA
                     DTGEQGLYHQGSRFLSYFELRLNGQRPLLLNSSVKLDNFVLSVDLTTHDYFDEGKVTV
                     LKGTVHVSRERLLWEAACHERMVVSNYGEQPVSLHLTLDFAADFADIFEARGFSRTRR
                     GEYLPPQVDATKAVLAYRGLDQVERRTGLSFKPAPKQLTSSQAEFLVELGPLELQVID
                     FTVTCEPGAPSHDQVAHTSVLAKADCSLRQMVLGRCLVSTTNESFNDWITRSALDLEM
                     LTLDNPEGPYPYAGVPWYSTPFGRDGLITAMHTLWVQPGMARSVLTYLAAAQATEVLP
                     DQDAEPGKILHEVRRGELAAIGDIPFGRYYGSVDSTPLFVALAGQYLRRTGDVQFIST
                     LWSNIRRALEWIDCYGDVDQDGFIEYQRNTAKGLANQGWKDSDNAVFHRDGQSAEGPI
                     ALCEVQAYVYSARLEAAAIADALGDKPHAQRLKQQAEELRDNFERYFWCESLGTYAMA
                     LDGNKRKCEIRASNAGHVLWSGIACDERAQATGRTLLAPESFSGWGVRTLAEGEARYN
                     PMSYHNGSVWPHDNAMVAMGLARYGMKWGALDILGALFSASRFMDLQRLPELMCGFPK
                     RRSEGPTLYPVACSPQAWASSSVFYLLQACLGLSLHYDAPHVRMHHPILPASIERMEI
                     RNLQANGGFIDLALYRHEHDVGVNVLRKDNSVEISVLV"
     gene            complement(2717..>3447)
                     /locus_tag="VN496_16350"
     CDS             complement(2717..>3447)
                     /locus_tag="VN496_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXF18579.1"
                     /translation="SISEAQRKSLPSAHWVATVHHGLPTDLLAFSPRKGTYLAFLGRI
                     SKEKRVDRAIRIACRTGLPLKIAAKVDPADVTYFNESIRPLLKAPGVELIGEIGDHEK
                     SAFLGGALATLFPIDWPEPFGLVMIESLACGTPVIAFKGGSVMEILDHGSTGFVVDSE
                     EEAIQAVERIETISRARCRNTFERRFSATRMAHDYVSVYRSVRTDHLHRLQSPDHAKT
                     IGQATASLPSRAMVAVAAPLEPLP"
BASE COUNT          762 a          967 c         1005 g          713 t
ORIGIN      
        1 ttcggggatg tcgaagaaat ggcatcacaa aggaccgcac cgaatagcac aaaagaaaag
       61 tgtcgaaaaa gtaacgcacg cattgtcgta cgttcgacag cgcgggaagg cgcaaagcgt
      121 cacagaatgc catattgaaa cggtggattc gttgcctgac atatcggatc gaacagcatt
      181 cccactttag ataggtcaaa aaatcttcgg tatcggtcgt gtaatattat ttttccgagt
      241 gcgcttaagc aggaggccac atggaaaaga cgaatggcat tttcgataac agggcaacaa
      301 tgcagcgtga ggcctggatg gatggtgaaa tcattggtgt atggcctgca gcgaaatgcg
      361 ggacagggct cgacacactg atgccgtggg aacgcgaggc tttggaagcg cgttggggcg
      421 cttatcccaa cccacctcct cgcgacacct agagaacaaa ctcctctaat gaacgcatcg
      481 ttccggtcac ttcactgcac gatgccggtg cgggcgcggt tcattagacg agcacagaaa
      541 tctcgacgct attgtccttg cgcagcacat tcacacccac atcatgttcg tgtcggtaaa
      601 gcgccaggtc gatgaagcca ccatttgcct gcagattccg gatctccatg cgttcgatgg
      661 atgccggcaa tatcgggtgg tgcatgcgaa cgtgtggcgc atcgtaatgc agtgacaaac
      721 cgagacaggc ctgaagcaga tagaacacgc tggaacttgc ccaggcttgt ggggaacagg
      781 ctaccggata cagtgtcggc ccttcgctgc gccgtttcgg gaaaccacac ataagctcgg
      841 gaaggcgctg caaatccatg aaccggctgg cgctgaacaa agcgccgagg atatcgagcg
      901 ctccccattt catgccgtaa cgcgccagtc ccatcgcgac catggcgtta tcgtgcggcc
      961 agaccgatcc gttgtgatac gacatgggat tgtagcgggc ctctccttcg gccagcgtgc
     1021 gcacgcccca accggagaat gattccggtg cgagaagagt gcgtccagtc gcttgcgcgc
     1081 gttcgtcgca ggcaattccg ctccagagca cgtgaccggc gttggaagca cggatttcgc
     1141 atttccgttt gttgccgtcc agcgccatcg catatgtgcc gagcgattca caccagaagt
     1201 accgctcgaa gttgtcacgc agttcctcgg cctgttgttt gaggcgctgc gcatgcggct
     1261 tatccccgag cgcatcggca atcgcggccg cttcgagacg cgccgaatag acgtaggctt
     1321 gcacttcaca caatgctatc ggtccctcag cggattggcc gtcacgatgg aacactgcat
     1381 tgtcggaatc tttccaaccc tgattcgcca gccctttcgc ggtatttcgc tggtattcga
     1441 tgaatccgtc ttggtcgacg tcaccatagc agtcgatcca ttcgagcgcg cggcggatgt
     1501 tggaccaaag cgtcgagata aactggacat cacctgtgcg ccttaagtac tggccggcca
     1561 gcgcgacgaa caaaggtgtc gaatcgacgc tgccatagta ccggccgaaa ggaatgtcac
     1621 cgattgcggc caactcgcca cgcctgacct cgtgcaagat cttgcctggt tctgcatcct
     1681 gatcggggag tacttcggtt gcctgagcgg cggccaggta cgtcagtacg ctgcgcgcca
     1741 tgccgggctg tacccagagc gtatgcatgg ccgtgatcag gccgtcacga ccaaacggtg
     1801 tgctatacca cggcacgcca gcatatggat aaggaccttc aggattatcg agcgtgagca
     1861 tttccaggtc caaagccgag cgggttatcc agtcattgaa tgactcattc gtggttgata
     1921 cgaggcatcg tccgagcacc atttgacgca gcgagcaatc ggccttcgcc aacacactgg
     1981 tgtgggctac ctgatcatgc gacggcgcgc caggttcgca cgtcacagtg aagtcgatta
     2041 cctgcaattc cagcgggccc agctcaacca aaaactcggc ttgcgaagag gtcagttgct
     2101 tgggtgccgg cttgaagctc aatccggtgc gccgctccac ctggtccagg ccgcgatagg
     2161 caagcaccgc cttcgtggcg tcgacttggg gcggcagata ttcgccacgg cgggtgcgtg
     2221 aaaatccgcg agcttcgaaa atgtccgcga aatcggcggc aaaatcgagc gtcaggtgca
     2281 ggctaaccgg ctgctcgccg taattggaga cgaccatgcg ttcatgacag gcagcctccc
     2341 aaagaaggcg ttcccgggaa acgtgtactg tgcctttgag tacagtgacc ttgccttcgt
     2401 caaagtagtc gtgcgtggtg agatccacgc tcagtacgaa gttgtccagc ttcacgctcg
     2461 agttcagcag cagcgggcgc tggccgttca accgaagttc gaaatacgaa agaaaacggc
     2521 ttccctggtg ataaaggcct tgctcgccgg tgtccgcaaa acgcacatcg ccgtagcggt
     2581 cgaagacgcc aaaagtctcc ccgtgcttaa gcactcgcgt cctgtcgtct ggaggaagtg
     2641 cgttgacgtg gttttgaccg tcgtccagcc gagcggcatc ggtcatgggc atccccgtga
     2701 aaaaatcaac ggcccttcag ggcagtggtt ccagaggcgc ggccactgcg accatcgcac
     2761 gagagggtaa ggatgctgtt gcctgtccaa tcgttttcgc atggtctggt gactggagac
     2821 gatgcagatg gtcggtgcgg acactgcgat agaccgagac atagtcatgg gccatgcgcg
     2881 tggcgctaaa gcgacgctca aaggtattgc ggcaccgtgc ccggcttatc gtttcgatgc
     2941 gttctactgc ctggatcgcc tcttcctcgc tatccacgac gaagccggtc gagccgtgat
     3001 ccagaatctc catgaccgag ccgcctttga acgcgatgac cggcgttccg catgcgagtg
     3061 attcgatcat gaccagtccg aatggttcgg gccaatcgat tggaaacaac gtagccaggg
     3121 caccgcccag aaaggcggac ttttcatggt caccgatttc accgatgagt tcgacgcccg
     3181 gcgctttcaa tagcggacgg attgactcgt tgaagtaagt aacgtccgcc ggatccactt
     3241 tagcggctat cttcagcggc aagccggttc gacatgcaat tcgaatggcg cgatcgactc
     3301 ttttctcttt ggatattcgc cccagaaatg cgaggtaagt cccttttcgc ggactgaaag
     3361 ccagcaggtc cgtgggcagg ccgtggtgta cggtggcaac ccagtgcgcc gagggcaggg
     3421 atttgcgctg tgcctcggaa atggaga
//
LOCUS       DATMZQ010000513         5405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1030594, whole genome shotgun sequence.
ACCESSION   DATMZQ010000513 DATMZQ010000000
VERSION     DATMZQ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030594"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VN496_16355"
     CDS             <1..1163
                     /locus_tag="VN496_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXF18580.1"
                     /translation="LREGVLMQAYFDHNATTPLDEEVLAQMLPFLRHEYGNASSRHDW
                     GTRAHRAIDRARQQVAALVNVQPAQVVFVSGGTEANNLFVRGAAAYLKPSQVVVSAIE
                     HPCVAKPARELAREGWRLRTLATDRLGKVEPADAELALREPTGLVSVMLANNETGVIQ
                     PVSEIAAMARDHKAWMHTDAVQALGKIPVDFEALGVDAMSLSAHKIYGPKGIGALVVS
                     NRLELKPIIHGGGHEAGIRSGTHNVPGIVGFGAACELASKRMQEIAKRTRDLRDRTEL
                     DLIALGARIFGRGAERIPNTVYFAFPEIDGETLVIELDKAGIAVASGAACSSTSTEPS
                     ATLLAMGVAPEIARGAVRVSFGVQNSGREVEQFLRKLGATVSRLRQLTAIAV"
     gene            1199..1528
                     /locus_tag="VN496_16360"
     CDS             1199..1528
                     /locus_tag="VN496_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXF18581.1"
                     /translation="MAITVTENAAKHIRNSVAKRGGIGLRLGIKTVGCSGMAYTFDYA
                     DEVGVNDELFRTGDVAVVVDRKNLEFVDGSVLDYAREGLNEFFKIDNPKATAMCGCGE
                     SFTVAKV"
     gene            1546..2982
                     /gene="sufB"
                     /locus_tag="VN496_16365"
     CDS             1546..2982
                     /gene="sufB"
                     /locus_tag="VN496_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXF18582.1"
                     /translation="MSAVIQNLVNQPYKHGFVTDIESDVVAKGLSEDVIRLISAKKGE
                     PEWLLQFRLSAFRHWLTMTEPTWPNVRYPKIDFQSISYYSAPKAREKLSSMDEVDPEL
                     LRTFEKLGVPMNERAALAGVAVDVIFDSVSVATTFRKKLAEAGVIFGSFSEAVKDHPE
                     LVRKYLGSVVPTGDNFYAALNSAVFTDGSFCFIPKGVRCPMELSTYFRINTQESGQFE
                     RTLIVAEEGAQVSYLEGCTAPKFDTNQLHAAVVELVALDNAEIKYSTVQNWYAGDSEG
                     KGGIYNFVTKRGLCKGVNSKISWTQVETGSAITWKYPSCVLQGDNSVGEFYSVALTNH
                     HQQADTGTKMIHIGRNTRSKIISKGISAGVSNNSYRGLVKIGAKAEGARNFSQCDSML
                     IGSKCGANTFPYIQVNNSTAKVEHEASTSKIGEDQLFYFASRGIGAEDAVSMIINGFV
                     QDVFVQLPMEFAVEATKLLGLKLEGSVG"
     gene            3003..3767
                     /gene="sufC"
                     /locus_tag="VN496_16370"
     CDS             3003..3767
                     /gene="sufC"
                     /locus_tag="VN496_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXF18583.1"
                     /translation="MLEVRDLTAKIEDRTILKGIHFTVRAGEVHAIMGPNGSGKSTFA
                     KVLAGHPAYEVMRGEVLFEGKNLLSLTPEERARAGVFLGFQYPVEVPGVSNSSFLRLA
                     YNTIQSARALEELDPLEFDDFVREKMKSLEMDPAFLERSVNAGFSGGEKKRNEILQMS
                     LLAPRLAILDETDSGLDIDALRVVASGVNKLHTPEMGLVLVTHYQRLLNYIEPDYVHV
                     MEGGRIIKTGDKSLALELEARGYDWVATESKGKAAA"
     gene            3767..5083
                     /gene="sufD"
                     /locus_tag="VN496_16375"
     CDS             3767..5083
                     /gene="sufD"
                     /locus_tag="VN496_16375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXF18584.1"
                     /translation="MHVQNQNNEYLEKLLQGSEAVADAGANWLRVLRMRAVERANEMS
                     VPTTRDEEWRFTDLSSLYKIPFRPVSNAPVLAESGLTDFFVPEAHTRLVFVDGHYAAN
                     LSSVAVQQGVTVASLANLPASAEAMLEMRLGKLASIDNDVFAAVNTAFLRDGALVHIA
                     RGYTSDAPIHLLFVASSKQAAAYPRVLVVAEPETECAIVEDFVGVGDDAAYLTDSVSE
                     IHVGKQARVSHVKLQREMHSAFHIANTSVNVASSGFYAMWTVALGARLSRHNLSIVQA
                     GEGTEFTVDGLALIGGGQLADMHSSIDHAHAYGRSRQLHKTVAGGAAHAVFNGKILVR
                     EGAQQTDSGQQSRNLLISDKAHVDTKPQLEIFADDVKCAHGATVGQIEAEELFYLKSR
                     GLSDTTARNLLTYAFAAQIIERIPVKSLANQLVKIVLEQTHMKGTA"
     gene            5080..>5405
                     /locus_tag="VN496_16380"
     CDS             5080..>5405
                     /locus_tag="VN496_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXF18585.1"
                     /translation="MSSSSSVLQAARPIATFDVERIRAEFPILNLKVNGKALAYLDNA
                     ASSQMPQPVIDRFVRYQTAEHANIHRGVHYLSETATAAYEAARVKIQRFINAREAREV
                     VYTRGTT"
BASE COUNT         1266 a         1357 c         1627 g         1155 t
ORIGIN      
        1 ggctgcgtga aggcgttctg atgcaagctt actttgacca caacgccaca acgccgctgg
       61 acgaggaagt tctggcgcag atgctgcctt ttctacggca cgagtatgga aatgcctcga
      121 gccgacacga ctggggcacg cgtgcccatc gcgccataga ccgggcgagg caacaagtcg
      181 cagctctcgt aaacgtgcag ccggctcagg tggtgttcgt ttccgggggt acagaggcga
      241 ataacctgtt cgttcgcgga gcggcggcgt acttgaagcc cagtcaggtg gtcgttagtg
      301 caatcgagca tccgtgtgtg gcgaagcccg ctcgcgaact tgcgcgggaa ggttggcgac
      361 tgcggacact ggctactgat cgcctgggta aagtcgagcc cgccgatgcc gagcttgctc
      421 tgcgcgagcc tactggactg gtttctgtga tgctcgcgaa caatgaaacg ggcgtaattc
      481 aaccggtttc tgaaattgcg gcgatggctc gagaccacaa agcgtggatg cataccgatg
      541 cggtacaagc cttgggcaag ataccggtgg atttcgaggc attgggtgta gacgcaatga
      601 gtttgtcggc acacaagatt tacggcccaa aaggaatcgg cgccctggtg gtgagtaatc
      661 gcctggaatt gaaaccgatc attcacggcg gaggtcacga agccgggata cgttccggga
      721 cgcataacgt gccaggcatc gtagggtttg gcgctgcctg cgaactggct tcaaaacgaa
      781 tgcaagagat tgcgaagcgc acgcgcgatc taagggaccg tacagaactg gatttgatcg
      841 cgctgggcgc gagaatattc ggaagggggg cggagcggat tccgaatacg gtgtatttcg
      901 cctttccgga gattgacggc gagacccttg tgatcgaact ggacaaagcg ggtatcgcgg
      961 tggcaagtgg tgcggcgtgt tcgagtacca gtacggaacc gagcgcgacg ctgctggcga
     1021 tgggcgtagc gccagaaata gcgcgtggcg cggtacgggt aagtttcggt gtccaaaaca
     1081 gcgggcgtga agtagagcaa ttcctgcgaa agctgggtgc cacggtatcc agactcaggc
     1141 aattgacggc aattgctgtc tgatcaagag aagcatccaa gttcagtcag gagcacaaat
     1201 ggcgataacg gttacagaga atgcggcaaa acacattcgc aactcagttg caaagagggg
     1261 tggcatcggc ttgcgccttg gtatcaagac agtcggatgc tcgggtatgg catatacctt
     1321 cgattacgcc gatgaagtcg gcgtcaacga cgagttattc cgcacaggcg acgttgcggt
     1381 ggtggtggac aggaagaacc tcgagttcgt ggacggatcg gtacttgact acgcgcgcga
     1441 aggtctgaac gaatttttca agattgataa tccgaaggca acggcgatgt gtggttgcgg
     1501 cgaaagcttc actgtcgcca aggtctgacg catcaggagc agcaagtgag cgcagtaatc
     1561 cagaatctcg tcaaccaacc gtacaagcat ggattcgtta ccgacatcga gtcggatgtc
     1621 gtagcaaaag ggcttagtga agatgtcatt cggcttattt ccgccaagaa gggcgaaccg
     1681 gaatggctgc tgcagttcag gctaagcgca tttcgccatt ggctcacgat gaccgaaccc
     1741 acctggccta acgtcaggta tccaaagatt gactttcaat ccatcagcta ctattcggcg
     1801 ccaaaggcca gggaaaagct ttcgtcgatg gatgaagtgg atcccgagct gctgcggact
     1861 ttcgaaaagc tcggcgtgcc catgaacgag cgggccgctc tggccggcgt tgcagtggat
     1921 gttattttcg acagcgtgtc ggtcgccacg accttcagga agaagcttgc cgaagccggc
     1981 gttatttttg gctcattttc ggaagcagtg aaagatcatc cggagctcgt gcgcaagtat
     2041 ttaggcagcg ttgtgcccac gggagataac ttctacgctg ctttgaattc ggccgtattt
     2101 accgacgggt cgttctgttt tattccgaaa ggcgtgcgct gcccgatgga gctgtcgact
     2161 tattttcgga tcaacaccca ggaatcgggc cagttcgagc gcacgctgat cgtcgctgaa
     2221 gagggcgcgc aggtatcgta tctggaaggc tgtacagcgc cgaaatttga taccaaccag
     2281 ctccacgccg cggtggtcga attggtcgcc ctggacaacg cggaaatcaa gtactcgacg
     2341 gtacagaact ggtacgccgg agattcagag ggcaaaggcg gcatttataa cttcgttacc
     2401 aagcgcggtc tgtgcaaagg cgttaattcc aagatttcct ggacacaggt cgaaaccggg
     2461 tcagctatta cctggaaata tccatcctgt gtcctgcaag gggacaactc ggtgggcgag
     2521 ttctattccg tcgcgttgac caatcaccat cagcaggcgg ataccgggac aaagatgatc
     2581 catatcggcc gcaatacgcg aagcaaaatt atttccaaag gtatttctgc cggggtttcg
     2641 aataatagtt atcgcgggtt ggtgaaaatt ggagcgaaag cggaaggtgc acgcaacttt
     2701 tcacaatgcg attccatgtt gatcggcagc aagtgcggcg cgaacacgtt tccgtatata
     2761 caagtcaata acagcacggc aaaagtcgag catgaggcgt cgacgtccaa aattggggaa
     2821 gaccagctgt tctatttcgc cagccgtggc attggcgccg aagatgcggt ctcgatgatc
     2881 attaacggat tcgtgcagga tgtattcgtg cagcttccta tggaatttgc ggttgaagcc
     2941 accaagctgc ttggactgaa gctggaagga agcgtcggat gatcaggcca aatgcgcccg
     3001 tcatgctcga agttcgcgat ctgaccgcga agatcgagga ccgaaccatc ctgaagggaa
     3061 ttcatttcac cgtgcgggcg ggagaagtcc acgcaatcat gggccccaat ggctcgggaa
     3121 agagcacatt cgcaaaagta ttggcagggc atccggctta tgaagtcatg cggggcgagg
     3181 tcttgtttga gggcaaaaat ctgctgagcc taacgccgga agagcgcgcc cgcgcagggg
     3241 tctttctcgg tttccagtat ccagttgaag tgcccggcgt aagcaatagt tctttcctgc
     3301 gactcgccta caacaccatt caaagtgccc gtgccttgga agaactcgat cctctcgaat
     3361 ttgacgattt cgtacgcgaa aaaatgaaat cgcttgaaat ggatcccgct tttctcgagc
     3421 gcagtgtcaa tgcgggtttt tccggtggag agaagaagcg caacgagatt ctgcaaatgt
     3481 cgctgttggc accacggctt gcgatactcg atgaaaccga ttccggactc gatatcgatg
     3541 ctctgcgggt tgtggcaagc ggagtcaaca agctgcatac gcccgaaatg ggccttgttt
     3601 tggtgactca ttaccagcgg ctgcttaatt acattgaacc tgattacgtt catgtgatgg
     3661 aaggcggccg cattatcaag acgggcgaca aatctctggc gctggaactg gaagctcgcg
     3721 gctacgactg ggtggctacc gaatccaaag gcaaggcagc ggcgtgatgc atgtgcaaaa
     3781 tcaaaataac gaatacctgg aaaagcttct gcagggtagc gaagcagttg cggatgcggg
     3841 agcaaactgg ttgcgcgtgc tgcgcatgcg agcagtggag cgtgccaatg agatgtccgt
     3901 gccaaccacg cgcgatgagg aatggcgctt taccgattta tcgtcactgt acaagattcc
     3961 atttcggccc gtttcgaacg cgcctgtcct agcggaatcc ggactgacag acttctttgt
     4021 tcctgaagcg cacacgcgat tggtttttgt ggacggacat tacgcggcca atctttcaag
     4081 cgtggcggtg caacaaggcg taacagttgc cagtcttgca aatttgccgg cgtccgccga
     4141 ggcgatgctt gaaatgcgcc tgggaaagct ggcctcgatc gataatgacg tgtttgctgc
     4201 ggtaaacacc gcattcttgc gtgacggcgc tttggtacat attgcgcgtg gctacaccag
     4261 tgatgcgccg atccatctgc tgttcgttgc cagttcgaag caggccgccg cgtacccccg
     4321 cgtgctggtg gtagcggaac ccgaaacaga atgcgcaatt gtggaagatt ttgtaggggt
     4381 cggcgacgac gcggcttacc tcactgactc ggtcagtgaa atccatgtcg gcaagcaggc
     4441 ccgcgtgtca catgtcaaat tgcagcgcga aatgcactcc gctttccata tcgcaaatac
     4501 atcggtgaat gtagcgagca gcgggtttta cgctatgtgg acggttgcgc tcggcgcgcg
     4561 gctttcccgg cataacctct ccattgtgca agctggcgaa ggaaccgaat tcacggttga
     4621 cggcctggca ttgattggtg gcggccagct tgcggacatg cacagcagca ttgaccacgc
     4681 gcatgcgtat ggtcgaagcc ggcaattgca caagaccgtt gcgggtggcg cggcacatgc
     4741 cgtattcaac gggaagattc tggtacgcga aggcgcgcag cagaccgatt cggggcaaca
     4801 atcgcgcaat ctcctgatat cggacaaggc gcatgtcgat accaaaccac agctggaaat
     4861 ctttgccgat gatgtgaagt gcgcacatgg tgctacggtc gggcagatag aagcggagga
     4921 gctgttctat ctgaaaagcc gtggcctttc cgacacgacc gcccgcaact tgcttaccta
     4981 tgcgtttgcc gcgcagatta tcgaacgcat tccggtgaag tcgttggcaa accagctggt
     5041 gaaaatcgtt cttgaacaga cacacatgaa aggcaccgca tgagttcgag cagcagtgta
     5101 cttcaggcgg cgcgcccgat cgccacgttc gatgtggagc gtatccgtgc ggagttcccg
     5161 atattgaatc tcaaggttaa cggtaaggcg ctggcgtacc tggacaacgc cgcaagtagc
     5221 cagatgccac agccggtgat cgatcgcttc gttcgctatc aaacagcgga acacgcaaat
     5281 attcatcggg gtgtgcacta tttgtctgag accgcgactg cggcttatga ggccgcgcgc
     5341 gtcaaaatcc agcgcttcat caatgcgcgg gaagcgcggg aagtcgttta tacgcgcggc
     5401 acaac
//
LOCUS       DATMZQ010000514         5767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_4833173, whole genome shotgun sequence.
ACCESSION   DATMZQ010000514 DATMZQ010000000
VERSION     DATMZQ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4833173"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1253
                     /gene="epsE"
                     /locus_tag="VN496_16385"
     CDS             276..1253
                     /gene="epsE"
                     /locus_tag="VN496_16385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HXF18586.1"
                     /translation="MGHQDKNYFASNSKLRALSWAALMVALIGTSLCAAARDTEPRPA
                     FAGQSSNGPQARDTPQARNSSQAANNPSPPNASERRSVAATRETLGAGDSIRITVFQN
                     PDLTTEARLSQAGAIVFPLIGEVALSGLTTAEAGSRIADQLKRGSFIVNPQVAVSILQ
                     VRSRQVHVLGQLSRPGNYILEDTASRLTDVLTLAGGISATGADTVTVLTNRNGKIERV
                     QIDVPAIFHGGDPSQNIQIENGDTIYVERAPVFYIYGEVQRAGAYRLEPNMIVMQALS
                     LGSGLTPRASERGIGIHRRMANGKFVKLDAKLTDPILADDIVFVKESLF"
     gene            1301..2731
                     /gene="epsF"
                     /locus_tag="VN496_16390"
     CDS             1301..2731
                     /gene="epsF"
                     /locus_tag="VN496_16390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HXF18587.1"
                     /translation="MNLQQFLLAMRARFGIFLLVLIATVVAAAVVSRAMPKTYKATAS
                     LLVAAKDEQSLSNVLQPLMLPQERLSYLQTQKDIIGSKKVARKVVQDLKLAEKASAVA
                     TLGDDETSRSSQEDRLVEGLLKNLKIETSQSNVMELSFSSPDPRYSMQVANGFAKAYI
                     DTMLEFRVEPAREAAAWFDEQLKGLRANLEDAQVKLTDNHQRQQIVSADERLDVENSR
                     LNALSDEVVRAQEQTMQWNSREQQAHRFLEEGAPADRLPEVLENPFIQRLKADFLHGE
                     TRLQEMSTQIGKNHPQYQRQASENQSLHEKLDAEMRKVVAGIETSARQARRREAALTQ
                     SLAEQRARMLSLKESRNEFTVLRRNVESAERAYDTAMQRSVVSQVESRANQTNVTVLN
                     AAGLPSKPASPRVSLNIAIALAVGLMLGIGMVLLLELSDRRVRCLKDLDKEWNAPLLG
                     ELKPWRPSSRLLGQSRSHHSALPNPG"
     gene            2741..3631
                     /gene="epsG"
                     /locus_tag="VN496_16395"
     CDS             2741..3631
                     /gene="epsG"
                     /locus_tag="VN496_16395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HXF18588.1"
                     /translation="MNTQDNVLPIEGSGRVINQQDGRLGNILVELGKIDRGDIDPILE
                     VQRVRGLRFGEAALSLRLIGRDDLRTAVAKQYGLPHLLPGGEEINSELVGAYEPFHPR
                     AEEMRALRTQLLIRWSNAQVRHRMLAVVSPGSGEGRSYVAANLAVAFAQLGERTVLLD
                     ADLRAPRQHRIFNVSDRVGLSAVLSGRADRSAVVALPQFGTLSLLPAGACPPNPQELL
                     LRPALAALLGELRNEFDVILFDTPPASLYADAQSVAFRAGSAIVLVRKDHTGVSDTAN
                     TIRALNDTGTRIVGTVFNAI"
     gene            3764..5182
                     /locus_tag="VN496_16400"
     CDS             3764..5182
                     /locus_tag="VN496_16400"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXF18589.1"
                     /translation="MSVLTALALLGVLSGGVVAIAGLNALYLCVSLIGCAFILRDFRI
                     GVALLIILMPISRSWMFPHAMLGVTGLNPFNLLLIGTLGSLLLQGLSEKNRGRLVPRP
                     LLWLYVVPIMAAGVLGAPHVHQIPSLLLDHNVIEFDTVGGYFRDLIFKPLMMVVFAML
                     VGAAMAKSKKTEQFLYPAVVAMWVMSVMVIVFVSLSGTSLQEMAKSESREVLSGLGMH
                     ANALGRLYVSAYALLLFTWVESKSAGFRFTLLISMAMVVVALILTFSRGAFLSFILVN
                     GLFLVWRRKAKTWLLFGVLAVSALLAMPGAVYDRALMGFGGGGSAEDISAGRIEYLWL
                     PLLPELARSPVYGHGLGAILWSEPMRTGAGITFIPVDHPHNGYLQAVLDMGIIGLVLL
                     CAFFAHVWKGFRRLSRDASLSPTMRGFYLGSAAGLASLLLSYATDSSLTPGPEQSFLW
                     LAVGMMYGEASRRLLKPAGNPS"
     gene            5271..>5767
                     /locus_tag="VN496_16405"
     CDS             5271..>5767
                     /locus_tag="VN496_16405"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HXF18590.1"
                     /translation="MEQRFTVLDSWRGIAACLVALFHLDAYSHLYDVPFLRNAWLFVD
                     FFFVLSGFIIAANYEKRLLEGYGTGRFLLLRLGRLYPLHFTMLMLFILFELLKGLGRT
                     VVSGSALADSIAPFGTEMEGPSTIFANLFLVHSLHLFRFLTWNHPSWSISTELYTYVV
                     FAVCLL"
BASE COUNT         1192 a         1640 c         1675 g         1260 t
ORIGIN      
        1 cctggcctca cttcaccgta tgagcccttg ggggaactgc acccgacgta gccgagtacg
       61 ctagtccgat cggaccagcg atggacatgg cgcataaatg tccgtggcct atcctgatgc
      121 gcagttcgat tcgccgccta acagctaaaa gatccgacga caacgtgtcg tgcagtccgc
      181 attctgaaac atgcgccaga caccggtgaa gcgcaccaat cacagccaga caggttcgac
      241 acacttcgag tcgtgatcct taagacgagg ttttgatggg acaccaggac aagaattatt
      301 tcgcatccaa tagcaaattg cgcgctttgt catgggccgc gctgatggta gcgttgatcg
      361 ggacctcact ttgcgcagcc gcccgcgata ccgagccgcg gccagcgttc gcgggccagt
      421 catctaacgg tccgcaggcg cgcgataccc cgcaggcccg caatagttcg caagcagcaa
      481 acaatccctc gcccccgaat gcctccgagc gccgcagtgt ggctgcgacc cgcgagacgc
      541 tgggagcggg tgacagcata cgcattaccg tatttcagaa tccggatctc acgacggaag
      601 cccgattgtc ccaggctggt gcaatcgtgt tcccgctcat cggcgaagtt gcattgagcg
      661 gattgacgac tgcggaagca ggaagccgga tcgccgacca actgaagcgc ggtagcttta
      721 tcgtcaaccc gcaggttgcc gtttcaatcc tgcaggtacg cagccgccag gtacacgtgc
      781 tgggccagtt gtcgcgtccc ggcaactaca ttcttgaaga tacggcctcg cggctgacag
      841 acgtactcac gcttgcggga ggcattagcg caaccggcgc cgatacggtg acggtcttaa
      901 ccaaccgcaa cggcaaaatt gaaagggtcc agatcgatgt gccggccata ttccacggcg
      961 gggacccctc acagaatatc cagatcgaaa acggcgacac catctatgtg gagcgcgccc
     1021 cggtgttcta tatctatggc gaagtacagc gtgctggtgc ctaccgcctt gagccgaaca
     1081 tgatcgtaat gcaggctctg tcgcttggaa gcgggctgac gcctcgcgca agcgagcgcg
     1141 ggattggcat ccaccggcgc atggccaatg gcaagttcgt gaagcttgac gccaagctca
     1201 ccgatccgat tctggcggac gatatcgtgt tcgtaaaaga gagcctgttc tagacctttg
     1261 aaccgctgta catgaacaac aacccatcgc gattgcaacc gtgaacctac aacaatttct
     1321 gctcgcaatg cgagcgcgtt tcggcatatt cctgctggtc ctgatagcca ccgtggtggc
     1381 ggccgcggtg gtaagccgcg ccatgccgaa aacctacaag gcaactgcat cgctactcgt
     1441 tgccgcaaag gacgagcaat ccttaagcaa tgtcttgcag cctctcatgc tcccgcagga
     1501 gaggctgagc tacctgcaaa cccagaagga catcatcggc agcaagaagg tagcgcgcaa
     1561 ggtagtgcaa gacctgaaac ttgcggaaaa ggcgtccgca gtagcgacgc tgggcgatga
     1621 tgagactagc cgctcatcgc aggaagaccg cctggtagaa ggcctgctga agaatctaaa
     1681 gatcgaaacc tcgcagagca atgtaatgga attgagcttt tcgtctcctg atccgcgtta
     1741 ttcgatgcag gtcgcaaacg gcttcgccaa ggcctatatc gacaccatgc ttgaatttcg
     1801 cgtggagccg gcgcgcgagg cggccgcgtg gtttgacgaa caattgaaag gcctgcgcgc
     1861 taatctcgag gatgcgcaag tgaagctgac cgataatcat cagcgtcagc aaatcgtctc
     1921 tgcagacgag cggctggacg tcgagaatag ccggctgaac gccttgtccg atgaagtcgt
     1981 gagggcgcag gagcaaacga tgcagtggaa cagccgggag cagcaagcac accggtttct
     2041 tgaagagggc gcgcctgcag acagactacc ggaggtactc gaaaacccat tcatacagcg
     2101 cctgaaggcg gattttctgc acggtgagac caggctgcag gaaatgtcta cacaaatcgg
     2161 caaaaatcat ccgcagtatc agcgccaggc ctcggagaat cagagtttgc atgaaaaact
     2221 ggatgccgag atgaggaaag tcgtggctgg cattgaaaca tcggcacgcc aggctcgtcg
     2281 gcgcgaggct gcactgacgc aatccctggc ggagcagcgt gcgcgaatgc tgagtcttaa
     2341 agaaagcaga aacgaattca cggtgctcag gcgcaatgtg gaatccgccg agcgggccta
     2401 cgacacagcc atgcagcggt cggttgtcag ccaggtggaa agccgtgcga atcagaccaa
     2461 tgtcacggtg ctcaatgccg ctggcttgcc gagcaagcca gccagcccac gcgtctcgct
     2521 caatattgcc attgccctgg ctgtcgggct gatgctgggc ataggcatgg tgctactgtt
     2581 ggaactctcc gatcgccgcg tacgatgtct taaagatctc gataaggaat ggaacgcgcc
     2641 tctgctcggc gagctcaagc cgtggaggcc ctcaagtcga ctgctgggtc agtcgagaag
     2701 ccaccatagc gcgttgccaa accctggctg aggattgtca atgaacacac aagataacgt
     2761 actgccgata gaaggctccg ggcgtgtaat caaccagcaa gacggacggc ttggcaatat
     2821 tctggtcgaa ctcggaaaaa tagatcgggg cgacatagat ccgatactgg aagtacagcg
     2881 cgtgcgcggg ttgcggtttg gcgaagccgc gctatcgctt cgcctcattg gcagggatga
     2941 cctgcgaacc gcagtcgcca agcaatatgg gttgcctcac ttgctgcctg gcggggaaga
     3001 gatcaacagc gaactcgttg gcgcctatga accgtttcac ccgcgcgcgg aggagatgcg
     3061 cgcactgcgt actcaactcc tcattcgctg gtctaacgcg caggtccggc acaggatgct
     3121 tgccgtagtc agcccgggat ctggcgaagg ccgcagctac gttgcagcca atctcgccgt
     3181 ggcatttgcc caactcggcg aacgcacggt tttgctcgac gctgatttgc gcgcaccgcg
     3241 gcagcatcgt atcttcaacg tgtccgatcg cgtcggtttg tccgcagtgc tgtcaggccg
     3301 cgcagaccga agcgccgtag tggcgcttcc gcaattcggc acgctttccc tgttgcccgc
     3361 cggcgcgtgc ccgcctaacc cacaggaatt gctgttgcgg cctgcattgg ccgcgttgtt
     3421 gggtgaactg cgcaacgaat tcgacgtcat tcttttcgat acaccgccgg ccagtttgta
     3481 tgcagatgca cagagcgttg cattccgggc gggcagtgca atcgttctgg tgcgcaagga
     3541 ccataccggg gtaagcgata ccgcgaacac gatccgggcg ctcaacgata ccggcacgcg
     3601 catcgttggc accgtcttca acgccatctg actgacctgg agaatggcac tacccctgca
     3661 acgccaacct cccggatgga tggccaggtg gcgcaccggc gcgtcgtccg ccgctgatag
     3721 cgtgccgcat gccggcaaaa cggccccttc cgtcctgcgt aaagtgagcg tcctaacggc
     3781 gcttgcgcta cttggcgtgc tgtcgggagg tgtagttgcc attgcgggac tgaacgcgct
     3841 gtatttatgc gtttcactca tcggctgcgc attcatcctg cgtgatttcc gcatcggcgt
     3901 ggcgttactc atcatattga tgcctatatc gcggagctgg atgtttccgc acgcgatgct
     3961 tggcgttacc ggcctgaatc cgttcaatct gctgctgatt ggcacattgg gctctctttt
     4021 gctgcaaggg ctttccgaaa aaaaccgggg gcgcctggtg ccgcgcccgc tgctatggct
     4081 gtatgttgtg cccattatgg cggcgggtgt gctgggggcc ccccatgtgc atcaaattcc
     4141 gtctttgctg ctcgatcaca acgtcatcga atttgacacc gtgggcggct actttcgcga
     4201 tctcatattc aagccgctca tgatggtggt attcgccatg ctggtgggtg cagcgatggc
     4261 gaaatccaaa aaaacagaac agttccttta tcccgctgtc gttgcgatgt gggtgatgag
     4321 cgtgatggtc atagtattcg tatcgttatc gggcacatcg cttcaggaaa tggccaaaag
     4381 cgagtcgcgt gaagttttgt cgggtctggg catgcacgca aacgcgcttg ggcgcctcta
     4441 tgtttctgct tatgctctgc tgctcttcac ttgggtggaa tcaaaaagtg ctggtttcag
     4501 atttaccttg ctgatttcaa tggccatggt tgtcgtcgcg ctcatcctca ctttttcacg
     4561 gggcgccttc cttagtttca ttctcgtgaa cggtctattt ctggtatggc gcaggaaagc
     4621 gaagacctgg cttttattcg gtgtcctggc cgtcagcgcg ttgcttgcga tgcccggcgc
     4681 cgtttacgat cgtgcgctga tgggtttcgg cggcggtggg tcagcggagg atatctccgc
     4741 cggacgcatc gaatatcttt ggctgccgct gctgccggaa ttggctcgca gtccggttta
     4801 cggccatggt ctcggggcga tactctggtc agagcccatg cgaactggag ccggcatcac
     4861 gtttattccc gtcgaccacc ctcacaatgg gtacctgcag gctgttctcg acatggggat
     4921 aattggcctg gtcctgctgt gcgctttttt cgcgcatgtc tggaagggat ttcgccggct
     4981 gagccgggat gcgagcctca gcccgactat gcgtgggttc tatctgggat ccgcagccgg
     5041 tctggcgagc ctgctgttgt cgtacgcgac cgatagcagc ctgacaccgg gtcccgaaca
     5101 atctttccta tggcttgcgg ttggaatgat gtatggcgag gcatccagac gccttttgaa
     5161 acccgccggc aatccgtcct gatccgtccg gaccagactc gcaagctgcg gcacgacttg
     5221 cattcgtgtc aagtttgcag cgtttccttt ccgcatcgtg gagcacacac atggaacaac
     5281 gattcacagt gctggatagc tggcgaggaa tcgcggcatg cctggtcgca cttttccatc
     5341 tcgacgctta cagccacctg tatgacgtgc cattcttgcg caacgcctgg cttttcgttg
     5401 atttcttctt tgtgttgagc ggcttcatca tcgccgccaa ctatgaaaaa cgactgctag
     5461 aaggctacgg cacggggcgt tttctgctac tgcgtctcgg caggttatat ccgctgcatt
     5521 tcacgatgct gatgctgttt atcctgtttg agcttctgaa aggccttggg cggactgtcg
     5581 tgtcgggcag tgcattggcc gattcgattg cgccattcgg caccgaaatg gaagggccgt
     5641 ccacaatttt tgccaatctg tttctggtcc acagccttca tctttttaga ttcctgacat
     5701 ggaaccatcc cagctggagc atcagcacgg agctctacac ttacgtcgtc tttgcagtgt
     5761 gcctact
//
LOCUS       DATMZQ010000515         4778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1034264, whole genome shotgun sequence.
ACCESSION   DATMZQ010000515 DATMZQ010000000
VERSION     DATMZQ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034264"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1054
                     /locus_tag="VN496_16410"
     CDS             131..1054
                     /locus_tag="VN496_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXF18591.1"
                     /translation="MATAFIKHPDCSLHDMGAGHPESPDRLAAIEDHLISTGLMEHLV
                     CTVAPEVTREQLLRVHDQNYVESIEAVAPQKGFVHLDPDTAMNPYSLSAAKYAAGAVV
                     KATDMVISGEVENGFCNVRPPGHHACRRKAMGFCLFNNIAVGVAHAIAVHGLDRVAIV
                     DFDVHHGNGTEDIFVGDQRVIMVSTFQHPFYPYSGAEGRSKQMVNIPLAAYSGSAQFR
                     GAVEREWIPALHDFAPQMIFVSAGFDAHRDDDMASLALSESDYAWVTRQIVDVAQRYA
                     DRRIVSTLEGGYNLEALARSVAAHVRGLCEI"
     gene            complement(1130..1513)
                     /locus_tag="VN496_16415"
     CDS             complement(1130..1513)
                     /locus_tag="VN496_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HXF18592.1"
                     /translation="MKLHLSQNARRNTVTAYGQGYVAVNGKRYESSLVVLPDRVISDW
                     NVTSVENLSEGTVGELAQLRPELVLLGTGDSLHFPDSKLLTVLLTAGIGTEVMDTHAA
                     CRTYNILAQEGRNVAAALVIPPMGA"
     gene            1615..2829
                     /gene="alaC"
                     /locus_tag="VN496_16420"
     CDS             1615..2829
                     /gene="alaC"
                     /locus_tag="VN496_16420"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HXF18593.1"
                     /translation="MAEFSRIKRLPPYVFNITAELKAAARRRGEDIIDMSMGNPDGVT
                     PKHIVDKLVEAAQRNDTHGYSMSKGIPRLRRAICNWYQTRFGVALDPESEAIVTIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAVIAGADIRSVRMTPGIDFIEELQRAIR
                     ESIPKPKMMILGFPSNPTAQCVEVDFFERVVALAKEHGILVVHDLAYADICFDGWKAP
                     SIMQVPGAREVAVEFFTMSKSYNMAGWRIGFMVGNKELVSALARIKSYHDYGSFTPIQ
                     VASIAALEGPQDCVLEICAKYQKRRDVLVRGLHEAGWMVQIPKASMYIWAEIPDAYKK
                     MGSLEFAKKILLEAKVAVSPGIGFGEYGDSYVRFALIENESRTRQAIRGIKEMFRQDG
                     LLRTAPQLRAAG"
     gene            2893..3348
                     /locus_tag="VN496_16425"
     CDS             2893..3348
                     /locus_tag="VN496_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF18594.1"
                     /translation="MLKRAFRIVQVTGSGREIIEESWLTKAEQVHRQLRPQLPTDYLQ
                     KMKLVFAGGGEMCVAVLDQRVIGVAVYREFENTHVGRRFYIDDLVTDESERSSGAGGQ
                     LIEYLQKVARDRGCPSLELESGTHRARAHRFYFREGFFISSFSFRKEFQ"
     gene            3345..4661
                     /locus_tag="VN496_16430"
     CDS             3345..4661
                     /locus_tag="VN496_16430"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXF18595.1"
                     /translation="MKPMNVGLLGIGTVGGGTFAVLRRNGEEISRRAGRAIVIKAVAD
                     KDISKARQIVDGSAEVINDAFALVSRSDIDIIVELIGGTRSAKDLILAAIENGKHVVT
                     ANKALLALHGNEIFLAAQKKGVMVAFEAAVAGGVPIIKALREGLTANRIEWIAGIING
                     TSNFILSEMREKGSNFSEVLAHAQKLGYAESDPTFDVEGIDAAHKLTIMAAIAFGIPM
                     QFDKVYTEGISRLTRQDIRYAEELGYRIKLLGITRRTGKGIELRVHPTLIPSKRLIAN
                     VEGVMNAILVKGDAVGATLYYGAGAGAEPTASAVVADLVDVTRMHTADPEQRVPHLAF
                     QPDRISDIAILAMEEVETSYYLRLRVIDRPGVLADITRILADQWISIDAMVQKEPGEG
                     EEQVDIIMLTHLTVEKNINGAIERIESLPAVSGKVTRIRLEELGRN"
BASE COUNT         1121 a         1219 c         1359 g         1079 t
ORIGIN      
        1 acggacaatg gattggacat tcgctttatc ggcaaacctt agaagggacg cgcgctactc
       61 gaatagttta cagtactgct gcgtgatgga cactcacggc gacaagggca ttccaggaac
      121 cgatgtgact atggcaaccg cgtttatcaa gcatccggat tgcagcttgc atgacatggg
      181 ggccggtcac ccggaatcgc cggaccggct cgcggcgatt gaagatcatc tgatctccac
      241 cggtctgatg gagcacctgg tgtgtactgt ggcgcccgaa gtaacgaggg aacaactatt
      301 gcgggtacac gaccagaatt acgtcgaatc aattgaagcg gtcgctcctc agaagggctt
      361 tgttcacctt gaccctgata ccgccatgaa tccatacagt ctatccgcgg caaaatatgc
      421 agcgggtgcg gtggtcaagg ccacggatat ggtcatttcg ggtgaagtcg aaaacggctt
      481 ttgcaacgtg cggccgccag gccaccatgc ctgccgccgc aaggccatgg ggttctgcct
      541 gttcaacaac attgcggttg gggtcgcgca cgcaatcgcc gtgcacggac tcgaccgcgt
      601 cgcgatcgtt gatttcgatg tgcatcatgg taacggcacc gaagacatct tcgtgggaga
      661 ccagcgtgta atcatggttt caacttttca acatcctttc tatccataca gcggcgccga
      721 gggtcgatct aaacagatgg tcaatattcc gcttgccgcc tacagcggaa gcgcacaatt
      781 taggggtgca gtagaacggg aatggatacc cgccttacat gacttcgcgc ctcagatgat
      841 atttgtttcc gctggattcg atgctcatcg agatgacgac atggcatcgt tagcgttgtc
      901 ggagagcgac tatgcgtggg taacacgtca aatcgtcgat gtggcgcagc gctatgccga
      961 tcgtcgtatt gtctcgacgc tagagggcgg atataacctg gaggcgctcg cgagaagcgt
     1021 ggcggctcat gtccgcggcc tatgcgaaat atgatagggc aggcgcgaag cacggaggcg
     1081 ttggaccagc gaagtgaatg atgttcgagc ccgctgttga ctgcggctct taagcgccca
     1141 tcggtggaat taccagcgcg gccgcgacat tacgtccttc ctgcgcgaga atgttgtagg
     1201 tgcggcaggc tgcatgggta tccataacct ccgtcccgat ccccgcagta agcagaaccg
     1261 tcagaagttt cgaatccgga aaatgtaaag aatcacccgt ccccagcagc acgagttccg
     1321 gcctcaactg agcgagttca ccaacggtgc cctcgcttag gttttcaacg ctggtcacgt
     1381 tccagtcgct gatgactctg tccggcagca ccaccaggct ggattcgtag cgtttgccgt
     1441 tcacggcaac atagccttgg ccataagcgg taacagtgtt gcgtctcgca ttctgagaaa
     1501 ggtgtagttt caatttaaca gtctttgctg tagcgcctgg tatccggtag gatttgggct
     1561 tctaatttat gaacagtcgg ttacggttca gagccgacgc gagtctcaat aaatatggcc
     1621 gagttttccc gcatcaagcg tctacctccc tatgtgttca acattaccgc ggaattgaag
     1681 gctgcagcac ggcggcgggg tgaagacatt atagacatga gcatgggaaa tccagatggt
     1741 gtgacgccga agcatatcgt cgacaaactc gtggaagctg cccagcgcaa cgatacgcac
     1801 ggatattcca tgtccaaagg catcccgcgc ttgcggcggg caatctgtaa ctggtatcag
     1861 actcgcttcg gagtcgcact ggatccggaa agcgaagcga ttgtgaccat cggatccaaa
     1921 gaagggcttg cgcacctgat gctcgccaca ctcgatcgtg gcgacaccgt gctggtaccc
     1981 aacccaagct atcccattca catttatggt gccgttattg cgggcgcaga catacgttcg
     2041 gtacgcatga cgccaggaat tgatttcatc gaggaattgc agcgggccat tcgtgaatcg
     2101 attccgaaac cgaagatgat gattcttggc tttccgagca acccgacagc acagtgcgtg
     2161 gaagttgatt tcttcgaacg agtcgtggcc ctggccaaag agcatggaat tctcgtggta
     2221 catgatctgg cctatgccga tatttgcttc gacggttgga aggcaccgtc catcatgcaa
     2281 gtcccgggag cgcgcgaagt cgccgtggaa ttttttacca tgagcaaaag ttacaacatg
     2341 gcaggctggc gcatcggttt catggtaggc aacaaggaac ttgtttcggc gcttgctcgc
     2401 atcaagagct atcacgacta cggcagcttc acacccatcc aggttgcgtc gattgcggca
     2461 cttgaaggac ctcaagattg tgttctggaa atttgcgcca aataccagaa gcggcgtgac
     2521 gtactggtcc ggggtctgca cgaggcgggt tggatggtgc aaatcccaaa agcatcaatg
     2581 tacatctggg cggaaatccc ggatgcttac aagaaaatgg gctcgcttga gttcgccaag
     2641 aaaatattat tggaagccaa ggtagccgta tctcctggaa ttggttttgg tgaatatggc
     2701 gattcttatg tgcgattcgc cctgatcgaa aatgaatcgc gcacgcggca ggcaatacgg
     2761 ggcataaagg aaatgttccg gcaagatggc cttttgcgta cagcgcccca actccgggca
     2821 gctggctgat cgatttagtt gcatactccc tgcatcgggc gcacagcgcc cgatttttat
     2881 tcagaggttt ccatgctaaa aagggcgttc cgcatcgtgc aggttacggg cagtggccgg
     2941 gaaatcatcg aagaaagctg gctgacaaag gcggagcagg tgcacaggca gttgcgtccg
     3001 caattgccga ctgactattt gcaaaaaatg aagctggttt ttgccggtgg cggcgaaatg
     3061 tgcgtggcgg tcctggatca acgggtcatt ggtgttgcgg tgtatcgcga gtttgaaaat
     3121 actcatgttg gacggcgttt ctacatagat gatctcgtga ccgatgaatc agaacgttcc
     3181 agcggcgccg gcggacaatt gatcgaatac cttcaaaagg ttgctcgcga ccgtggctgt
     3241 ccatccttgg aactggagtc aggcacgcat cgcgctcgtg cgcatcgttt ctatttccga
     3301 gagggattct ttatctcgtc attctcgttt agaaaagaat tccaatgaaa ccgatgaatg
     3361 ttggcctgct gggtatcggc accgtaggcg gcggaacctt cgctgtcctg cgccgtaacg
     3421 gcgaggagat cagtcgtcgc gccggccgcg ccatcgtaat caaggctgtg gccgacaagg
     3481 atatttcgaa agcacgccaa atcgtcgacg gcagcgctga agtcattaac gatgcgtttg
     3541 ccctggtgag tagatcggac atcgatatca ttgttgagtt gatcggcggt accagatctg
     3601 caaaagatct gatcctcgca gccattgaaa acggcaagca tgtcgttact gcgaacaagg
     3661 cgctactggc gcttcacggc aatgagattt ttctggccgc gcagaagaaa ggcgtaatgg
     3721 tggccttcga ggcagcggtc gctggcgggg tgccgataat caaggcactg cgcgaagggt
     3781 tgacggccaa tcggatcgaa tggattgccg gcatcataaa cggcacgtcg aacttcattc
     3841 tttcagaaat gcgggagaag ggttcaaatt tcagcgaagt gctggcacat gcgcaaaaac
     3901 tgggttatgc ggaatcagac ccaaccttcg acgtcgaagg catcgacgcc gcgcacaaac
     3961 tgaccatcat ggctgcgatc gcttttggca ttccgatgca gtttgacaag gtgtatacgg
     4021 agggaatttc ccgactcaca cgacaggaca tccgctacgc ggaagaactt ggctatcgca
     4081 ttaagctgtt gggcataacc cggcgcactg gcaagggaat cgaattgcgt gtgcacccga
     4141 cgctgattcc atccaaaaga ttgattgcca atgtggaggg cgtcatgaat gcgatcctcg
     4201 tcaagggtga tgcagtcggt gctacgcttt actatggagc cggcgcaggc gctgagccga
     4261 ctgcctcagc cgttgtcgct gatctggtgg acgtcaccag aatgcacacc gcggatcccg
     4321 aacaacgggt gccacatctc gcatttcagc ctgatcgcat ctccgatatc gcaattcttg
     4381 caatggaaga ggtggagacc tcgtattacc tgcgcttgcg cgttatagat cgtcccggtg
     4441 tattggccga cataacgcgc attttggcgg atcaatggat ttcgatcgat gccatggtgc
     4501 agaaggaacc cggtgaaggc gaggagcagg tcgatatcat catgcttact cacctgaccg
     4561 tcgaaaaaaa catcaatggt gctatcgaac gcatcgaatc gctgccagcg gtatctggca
     4621 aagtgacgcg aatccgcctc gaagaacttg gacgtaacta aaacatgctg aatcattaca
     4681 atggccttat cgatcgctat cgggagcgtc ttcccgttag cgcgaccact ccgattgtca
     4741 gtttgaatga aggcaatacg ccgctcattg aactgctt
//
LOCUS       DATMZQ010000516         4285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2324994, whole genome shotgun sequence.
ACCESSION   DATMZQ010000516 DATMZQ010000000
VERSION     DATMZQ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324994"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1595
                     /gene="tilS"
                     /locus_tag="VN496_16435"
     CDS             252..1595
                     /gene="tilS"
                     /locus_tag="VN496_16435"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXF18596.1"
                     /translation="MNEELAAIVDGVLDAHAVSGTHVAIALSGGMDSVVLLDLLHQCG
                     ATRQLSLSAIHVNHQISPHAAKWEAFCRSLCQQREIPITVQRVRVVAGGMGFEAAARQ
                     LRYQAFASIDADFVALAHHLDDQVETFLLQLLRGAGPKGLAGMPVVRPQQSGQKDNTA
                     PPLILRPLLEVRRNQINVYAKARQLVWVDDDSNGDSRFDRNYLRNEVLGKLTGRFPGY
                     RETLARAARNLADHAVLAEELARIDAQSLDRGTVSAERLRQLSDVRALNLLRQLFADR
                     GMQMPPRIRLEEALRQCRGASRDAEIQVNFGDARLRCYRDRVELVAFAADMPADWQSR
                     WDGQHELMLPDGLGRLRSRTVVGEGIARRHFDIRAATVRGRSGGERMQPGQNRPTRAL
                     KNLLQEHAVPPWERSRMPLVFFGEQLAWVPGIGVAAEFRAGATEDGIAPEWDPGC"
     gene            1640..2638
                     /locus_tag="VN496_16440"
     CDS             1640..2638
                     /locus_tag="VN496_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXF18597.1"
                     /translation="MKIIFFLLSLLTGFGSANAVEPVIIKFSHVVANDTPKGKAAEKF
                     KQLAEQYTNGRVKVEIYPNSQLYKDREEMEALQRGAVQLLAPSVSKFGPLGVREFELF
                     DLPYLIPDAATVDRVTDGEIGKKLFARIEPKGLKGLAYWDNGFKQFSANRPLRKPEDF
                     RGLKMRIQSSKVLEEMMRELGALPQVMAFSEVYSALQQKVVDGTENPVSNLYTQKMYE
                     VQSNLTISNHGCLVYAVITNRKFWEGLPADIRGQLEKAMSEATRYERDIAKQENDNAL
                     AAVKASGKTQVYVLSEAERKTLREALLPVQTKFESVVGKDNLEAIRRIAVESNSKK"
     gene            2645..3181
                     /locus_tag="VN496_16445"
     CDS             2645..3181
                     /locus_tag="VN496_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXF18598.1"
                     /translation="MIRTLNRILDHIEEWLIAGFMAAATLIIFAAVLHRQSAALPSVW
                     RYASALHFSWAQELCIYLFIWMAKFGAAYGVRTGIHIGVDVLVNALRPASRRVLIAIG
                     LLLGAFFTAAIAFMGARWVLFVHGTGQISPDLEWPMWVIYLCIPLGSSLMCYRFLQVL
                     TRFLRSGDLPSRTLDEAA"
     gene            3178..>4285
                     /locus_tag="VN496_16450"
     CDS             3178..>4285
                     /locus_tag="VN496_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXF18599.1"
                     /translation="MNVTLILVILFVLLLTGTPISIALGMTVLTFLALFSSFSMDTVD
                     IVSQRLFTGLESFAIMAVPFFVLSGSFLTEGGIARRIIRFALALVGWMHGGLAIAAIL
                     ACTFFATISGSSPATVVAIGSIMLPAMVKHGYPKRFSVGVITTSGSLGILIPPSIVMI
                     IYAVSTSESAGKLFIAGIVPGILLAAVLMLVAYVTARKKNYPTLPRASGNEIWASFKD
                     AFWGLMLVVIVMGGIYGGIFTPTEAAAVSAVYAFVAAVFIYRGLSLRQVPKVLLSAAN
                     TSAMLLYIITNAILFSFLLTSEQIPQALSHWVVDQHLSAWTFLIAVNIALLVAGQFME
                     PSSIILILAPLLVPIARNLGVDAIHLGVIMTVNME"
BASE COUNT          852 a         1208 c         1262 g          963 t
ORIGIN      
        1 gtcgctgaac ctcccggtgg cgctcatcgg gatccgacga ccatcatgca gactttgcgc
       61 aaatcgctca tggacgcgct ttccgaagct caggaaaggc cgatcgatgc actgctggaa
      121 acgcgcttcg agcgcctgat ggcgcatgga aagttcaggg aagcggcggt gcgctgaaac
      181 gtcgcgctcg cttatcattc ttgccgatag cccgcacaag cgcggttatc gtactttgag
      241 tcccaataat catgaatgag gaacttgcgg caatcgtcga cggtgtgttg gacgcgcacg
      301 ccgtaagcgg aacgcatgtc gccatcgcgc tcagtggcgg catggattcc gtggtcctgc
      361 tcgatctgct gcatcaatgt ggggccacga gacagttgtc gctttctgcc attcacgtga
      421 atcatcagat cagtccgcac gccgccaagt gggaagcgtt ttgccgatca ctctgccagc
      481 agcgtgaaat cccgatcacg gtacagcgtg tacgcgtcgt tgcggggggc atgggtttcg
      541 aagcggcagc gcgtcagttg cgctaccagg cctttgcttc aatcgacgcc gatttcgtcg
      601 cgcttgcgca ccaccttgac gaccaagtgg agacttttct gctgcaactg ttacgcggcg
      661 ctggtcccaa aggtctggcc ggcatgccgg ttgtcaggcc ccagcaatcc ggtcagaaag
      721 acaacaccgc gccaccgctg attcttcgcc cgctgcttga agtgcgacgg aaccagatca
      781 acgtctatgc caaagcgagg cagctggtct gggtggacga cgacagcaat ggcgactcgc
      841 gcttcgaccg caattacttg cgcaacgagg tcctcggaaa gctgactggt cgtttcccgg
      901 gttatcgcga aacgctggca cgcgctgcgc gcaaccttgc ggaccacgcc gtcctcgcag
      961 aagaattggc gcgtatcgat gcacaatcac tggatcgcgg cacggtctct gcagagcggt
     1021 tgcggcagtt gtccgatgta cgcgcgctaa acttgttgcg tcagctcttc gccgaccggg
     1081 ggatgcagat gcctccacgc atccggctcg aagaagcctt gcgccagtgt cgcggtgcca
     1141 gcagagacgc ggagatccag gtgaacttcg gggatgctcg cctgcgttgt tatcgcgacc
     1201 gtgtcgaatt ggtggcgttt gccgccgata tgcccgccga ctggcaatcg cgctgggacg
     1261 gtcagcatga gttgatgttg cccgatggct tgggccggtt gcgttctcgc acggtcgtcg
     1321 gcgaaggcat tgcaaggcgg cactttgaca tccgtgcggc gaccgtccgt ggccgaagcg
     1381 gcggcgagcg catgcagcca ggccaaaacc ggccgacgcg agcgctgaag aatttattac
     1441 aggagcatgc cgtccctccc tgggagcgca gccggatgcc attggttttt tttggcgagc
     1501 agcttgcgtg ggtaccgggc attggcgtgg ccgcggagtt ccgtgccggc gccacggaag
     1561 atggcatcgc gccggaatgg gaccccggct gctagaatcg cccaggaaaa aaacgtcaac
     1621 tgtcccgccc atgagaacaa tgaaaataat cttcttttta ctatcgctgc tgactggctt
     1681 cggcagcgca aatgctgtcg aaccggtcat catcaaattc agccatgtgg tcgcaaacga
     1741 caccccgaag ggaaaggcgg ccgagaaatt caagcaattg gccgagcaat ataccaacgg
     1801 acgcgtcaag gttgagatct atccgaacag tcagctgtac aaggaccgcg aggaaatgga
     1861 agcactgcag cgcggcgcag tgcagctatt ggcgccgtcc gtctcaaaat tcgggccgct
     1921 gggtgtgcgc gaatttgaac tgttcgatct gccgtattta atcccggatg cggcaaccgt
     1981 cgatcgggtc accgacggtg aaatcggcaa gaagctgttc gccaggatcg aacccaaagg
     2041 gctcaagggc ctggcgtact gggataacgg tttcaaacaa ttttccgcaa accgcccgct
     2101 gcgcaagccg gaggatttcc gcggattaaa aatgcgcatt cagtcttcca aagtgctgga
     2161 ggagatgatg cgcgagctcg gagcgttgcc acaggtcatg gctttctcgg aggtctactc
     2221 agcgctgcag caaaaggtag tcgatggaac ggaaaatccg gtttccaatc tttacaccca
     2281 gaaaatgtac gaggtgcaat ccaacctgac gatttccaat cacggttgcc tggtctacgc
     2341 ggtgattacc aaccggaaat tctgggaagg tttacctgcc gacatccgcg ggcagctgga
     2401 aaaggcaatg tctgaggcga cgcgctatga gcgcgacatc gccaaacagg agaacgacaa
     2461 cgcgctcgca gccgtcaagg cctccggcaa aacgcaggtc tatgtattgt cagaagcaga
     2521 gcgcaagaca ttgcgggagg cgttgctgcc ggtacagaca aagtttgaat ctgtcgtcgg
     2581 caaggacaac ctcgaagcga tacgtcgcat tgccgtcgag agcaattcga aaaaataata
     2641 agccgtgatc cggacactga atcgcattct cgatcatatc gaggaatggc tgattgccgg
     2701 attcatggcc gccgcgacac tgatcatctt cgccgcagta ttgcaccggc aatccgcggc
     2761 tctgccctcg gtatggcgat atgcctcggc gctgcatttc tcctgggccc aggagttgtg
     2821 catttacctc ttcatctgga tggcaaaatt tggagcggct tatggggtgc gtaccggtat
     2881 ccatatcggc gtcgatgtgc tggtcaacgc gttgcggcct gcctcgcgca gggtgctgat
     2941 agccataggg ctgttgcttg gcgctttctt taccgccgct atcgccttca tgggtgctcg
     3001 ctgggtcttg ttcgtccacg gcaccggtca gatttcgccc gatcttgaat ggccgatgtg
     3061 ggtgatttac ctctgcatcc cgctcggttc atcgctcatg tgttatcgct tcctgcaggt
     3121 gcttacccgc ttcctgcgct ccggtgatct accctcgcgg acgctggacg aggcagcatg
     3181 aacgtcacct tgatactcgt catccttttt gtattgctgt tgaccggcac gccgatttcg
     3241 atagcgctcg gaatgacagt cctgacattt ctggcgctgt tctcatcgtt ctcgatggac
     3301 acggtcgaca tcgtttcgca gcgactattc acggggctgg agagcttcgc gatcatggcg
     3361 gtgccgtttt ttgtgctgtc gggcagcttt ctcaccgagg gtggcatcgc gcggcgaatc
     3421 atccgcttcg ccctggcgct cgtcgggtgg atgcatggag ggcttgccat tgcggccatc
     3481 ctggcctgca cattctttgc gaccatctcg ggctccagtc cggccaccgt cgtggcgatt
     3541 ggctccatca tgttgccggc aatggtcaaa cacggatatc cgaaacggtt ttcggtaggc
     3601 gtgatcacca cctcgggttc gcttggcatc ctcattccgc catcgatcgt gatgatcatc
     3661 tacgcggtca gtacttcgga gtctgccgga aaacttttca tcgccggcat agtgcccggc
     3721 attctgctgg cagcggtgct tatgctcgtc gcttatgtga ccgcccgcaa aaaaaactat
     3781 ccaactttgc cgcgtgcatc cggaaatgaa atctgggcgt ccttcaagga tgcattctgg
     3841 ggattgatgt tggtggtgat tgtcatgggc ggaatttatg ggggcatatt tacgcctacc
     3901 gaagcggccg ccgtcagcgc ggtctacgcc tttgtcgcgg ccgtgttcat ttatcgcggc
     3961 ctttcactga ggcaggttcc gaaagtcctg ctttcggcgg ccaatacgag cgcaatgctg
     4021 ctctacatta ttaccaacgc cattctgttt tctttcttgc tgacctcgga acagattccg
     4081 caagcgctgt cgcactgggt ggtagaccag catctttcgg catggacgtt cctgattgcg
     4141 gtgaatattg cattactggt ggccggccag ttcatggagc ccagctcaat aatcctgatc
     4201 ctggcgccct tgctggtacc gatcgcgcgg aatctgggcg tagatgcaat tcatcttggc
     4261 gtcatcatga cggtgaacat ggaga
//
LOCUS       DATMZQ010000517         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1635384, whole genome shotgun sequence.
ACCESSION   DATMZQ010000517 DATMZQ010000000
VERSION     DATMZQ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635384"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN496_16455"
     CDS             <1..228
                     /locus_tag="VN496_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18600.1"
                     /translation="CFGSCHGISSPLEWRPVADETRASELINWVRATNPKLADIDEQV
                     PSAIRANGPHIFTQPPCAMFSGSTQVRKSCD"
     gene            327..470
                     /locus_tag="VN496_16460"
     CDS             327..470
                     /locus_tag="VN496_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18601.1"
                     /translation="MQEKSNGVITFSISDVLDFNRVADQKAKPAHPGRAGGTVALVGE
                     TRT"
     gene            467..1003
                     /locus_tag="VN496_16465"
     CDS             467..1003
                     /locus_tag="VN496_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXF18602.1"
                     /translation="MSLDIRWLAPVALVALSPQCIAAKYMSVEQARTLIFPLADEFIA
                     KPLQLTPEQMQEIEKLSAVPGRTAQHNVWQVLSQGKMIGWFFVDQVVGKHELITYALG
                     LNADCSVRQFQIVEYLEAYGYQVRELKWRDQFVGKTADSPLQVGTDIGNISGATLSVR
                     HITNGIKRLLYLCQTVLR"
     gene            1014..1841
                     /locus_tag="VN496_16470"
     CDS             1014..1841
                     /locus_tag="VN496_16470"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXF18603.1"
                     /translation="MRPAMGTLVHVAAIGPDSCATQEAVDAALDDIARIERLMSFHDT
                     ASDLARLNREAWRRPQTVHPWTWAVVRRALRFARASNGLFDITLAPLLIREGLLPGAV
                     AASPECGHWRHIELLPDCRISFTRPMLLDLGGIAKGFAVDQAIFQLRKRRCTDGIVNA
                     GGDLRCFGRVPRPIHLRRRDALVKVAELRCGAVATSAPRTTHSGRLAQPLGSIFNPHT
                     RHAWDAGAAVMVAAPSCVVADALTKVAALAGPACAPLLARFGAHARWDTEPASADWR"
     gene            1994..>2287
                     /locus_tag="VN496_16475"
     CDS             1994..>2287
                     /locus_tag="VN496_16475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXF18604.1"
                     /translation="MMRILVRIGCGLSLLLLFGSGLPAAQPTGEPIKVGAVLSLTGPG
                     AGLGGPERNGILLAEKRINERGGIRGRPLQILIEDDGSKPDIAKSKAESLIHGD"
BASE COUNT          461 a          685 c          697 g          444 t
ORIGIN      
        1 tgcttcggaa gttgccatgg catttcatct ccgctggaat ggcggccggt cgccgatgag
       61 acgcgagcaa gcgaattaat caactgggtg cgcgcgacca atccaaagct cgcggacata
      121 gatgagcaag ttccatccgc gattcgggcg aacgggccgc acatcttcac gcagccgcct
      181 tgcgcgatgt tttctggttc aacccaggtg cgcaagagct gcgactgaag cagcagaaca
      241 ggcacacatc ttcaatccgg aggctgtgcg atactgtgcc gcgcaccggc cttgcccttc
      301 gcggaagggc caaataaaaa actggcatgc aagaaaaatc aaacggcgtt attacgttct
      361 caatatccga cgtgcttgac ttcaaccggg tggctgatca gaaggcaaag ccggcccatc
      421 caggccgcgc gggcgggaca gttgcgctcg tgggggagac acgcacatga gtctggatat
      481 ccgctggctt gctccggttg cgctggtcgc gctgtcgccc cagtgcatag ccgccaagta
      541 catgagcgtc gaacaggcgc gcacgctgat tttcccgctc gccgacgaat tcatcgccaa
      601 gccgctgcaa cttacgcctg aacagatgca ggaaatcgag aaactgtcag ctgtgccggg
      661 acgcactgcg cagcacaacg tctggcaggt gctaagccag ggaaagatga tcggctggtt
      721 cttcgtggac caggtcgtcg gcaaacacga actgattacc tacgcattgg gccttaatgc
      781 agactgcagc gtgcgccagt tccagatcgt cgagtatcta gaggcctatg gctatcaggt
      841 ccgtgaactg aagtggcgcg accaattcgt cggcaagacg gctgatagcc cgcttcaggt
      901 cggtaccgat atcggcaaca ttagcggtgc aacgctgtca gtgcgccaca taacgaatgg
      961 catcaagcgc ctgctctacc tctgccagac cgtcctgcgc tgagttttcg cgcatgcggc
     1021 cagcgatggg cacgctggtc catgttgcgg caatcggccc cgacagctgc gccacccagg
     1081 aagccgtcga tgccgcactc gacgacattg ctcgcataga gcgattgatg agttttcatg
     1141 atacggcgag cgatctggcg cggttgaatc gggaagcctg gcgcagaccg caaacggtgc
     1201 atccctggac ctgggccgtt gtgaggcgcg ctttgcgttt cgcgcgcgcc agcaatggcc
     1261 tgttcgatat cacgctggca ccgctgctga ttcgtgaagg tttgcttccg ggtgcggtgg
     1321 ctgcatcgcc ggaatgcggt cattggcggc atatcgaact gcttcccgat tgcagaattt
     1381 ccttcacgcg acccatgctg ctggatttag gcggcatcgc caagggcttc gcagtggatc
     1441 aggcgatctt ccaactcaga aaaagaagat gcactgacgg aatcgtaaac gcaggcggtg
     1501 atctgcgctg cttcgggcga gtgccacggc ccatccatct tcgccgccgc gatgcactgg
     1561 tcaaggtggc cgaactgcgg tgcggtgcag tcgccacttc ggccccgcgt acgacgcact
     1621 ccggacgtct ggcacaaccg ctcggcagca tcttcaatcc ccacacgcgc cacgcctggg
     1681 acgccggcgc cgccgtgatg gttgctgcgc cgagttgcgt ggtggctgat gcattgacga
     1741 aggtcgctgc tcttgcgggc cccgcatgcg cgccattgct ggcgcgtttc ggcgcccacg
     1801 cccggtggga caccgaacca gcatctgccg actggcgtta agtcgcgcca ccgcatcatt
     1861 tgcaggttgt ctctgcaacg tcaccagcga gcaaaatgct gctggcttgc ggcatactag
     1921 tgctgggtgg cggggatgtg ccgttgtacg ggagcggcac agtgacaaca atctgttaag
     1981 ggaggcttca cccatgatgc ggatcttggt gcggataggc tgcggccttt cactattgct
     2041 tttattcggc tcgggcctgc cggcagcaca gccgacaggc gagccgatta aggtcggagc
     2101 agttctttcg ctgaccggcc ccggtgcagg tcttggcggt ccagaacgca acggcatcct
     2161 gctagccgaa aaacgcatca acgaacgcgg cggtatccgt ggccgtccgc tgcaaatcct
     2221 gatcgaagac gacggctcca aaccggacat cgccaaaagc aaggccgaat ccctgatcca
     2281 cggggac
//
LOCUS       DATMZQ010000518         9996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2331615, whole genome shotgun sequence.
ACCESSION   DATMZQ010000518 DATMZQ010000000
VERSION     DATMZQ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331615"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..877
                     /locus_tag="VN496_16480"
     CDS             350..877
                     /locus_tag="VN496_16480"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HXF18605.1"
                     /translation="MSQHLPAAAEPHTSDAPFRFIARRNNSFPLTANLALFGSIAAVM
                     LVIALGFAMAGAWPIFLFSGLELTLVYLAIRHMSKHAQDYESISVHDDKLVVVIKSKD
                     MQRCVEFNPYWVQVVGGASHGVRDGALILRSHGKDVAFGVHLTGEQRVELAHRLKHQL
                     RIRRHPDCERAQLTP"
     gene            887..2470
                     /gene="ctaD"
                     /locus_tag="VN496_16485"
     CDS             887..2470
                     /gene="ctaD"
                     /locus_tag="VN496_16485"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HXF18606.1"
                     /translation="MSAVIEHGHGDAHAGYPGGILRWVKTTNHKDIGTMYLWFSFVMF
                     IVGGTMAVLIRAELFQPGLQFLDPQFYNEMVSTHGLVMIFGAIMPAFVGFANWLIPIQ
                     IGASDMAFPRLNNWSFWLLIPGSLLLTGALFAPGGGNGAGWTMYPPLSVQHGMGMDMT
                     IFAIHILGMSSIMGSINIITTILNLRAPGMTLMKMPLFVWTWLITAYLLVAVMPVLAG
                     AVTMTLTDRHFGTHFFNAPGGGDPIMFQHLFWFFGHPEVYIIAIPAFGVISQVIPAFS
                     RKPLFGYTSMVYAVSAIAILSFLVWAHHMFTTGLPVTGQLYYMYATMLIAVPTGVKVF
                     NWVSTMWKGSLSFETPMLFAIGFLLLFTIGGFSGLVLAIVPIDIQVHDTYYVVAHFHY
                     VMVAGALFSAFAGIYFWLPKWTGHMYNEVLGKWHFWLSFIFFNMIFFVQHFLGLAGMP
                     RRIADYAPQFTDFNVISSLGAFGFGLSQLLFLYVVVNCIRRGEPAPQKPWEGADSLEW
                     THLPTPAPYHSFETPPLLR"
     gene            complement(2437..2748)
                     /locus_tag="VN496_16490"
     CDS             complement(2437..2748)
                     /locus_tag="VN496_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18607.1"
                     /translation="MAQESERSAALVELSDRLVDEASKREMIAVARVLALHVAHYRSR
                     YGIIPLRESMLVLLNDTAHETLADGVQVLIDAVRAVAMPAGPTNTAASSYLSNGGVSK
                     E"
     gene            complement(2825..3181)
                     /locus_tag="VN496_16495"
     CDS             complement(2825..3181)
                     /locus_tag="VN496_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18608.1"
                     /translation="MLFAVVPAYERGRRYTDKEFDAKAALVGELMIYEAAREAGGPLR
                     RIAGLTTPMGHLLKPILEPRLTGMTVLSFSLRGLEELETSRGYVYVLQEWRISEARHA
                     QKGYCIGDTPQDRKQG"
     gene            complement(3863..4054)
                     /locus_tag="VN496_16500"
     CDS             complement(3863..4054)
                     /locus_tag="VN496_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18609.1"
                     /translation="MLLHFKFSWVIDASLWKPEQGSAYRLDGRSGVVVASLLRSVFDE
                     VAPDDEFASPPLLLLVLLD"
     gene            complement(4057..4377)
                     /locus_tag="VN496_16505"
     CDS             complement(4057..4377)
                     /locus_tag="VN496_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18610.1"
                     /translation="MLFLLLDEELVPESAVGPLVLLPELLFPAGLPLFAPVLPDGLEV
                     DPEEFAADPDGLDEFAADPDGLDEFEDADLPALDDPGWLAWLLRSVHAARPKIVATTA
                     AEIT"
     gene            complement(4494..4676)
                     /locus_tag="VN496_16510"
     CDS             complement(4494..4676)
                     /locus_tag="VN496_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HXF18611.1"
                     /translation="MNERNQDDRSQKIDLNDPAQLHYWTTSLAVTEAQLRAAIKSVGN
                     EASKVRDYLGKTVARP"
     gene            complement(4761..6029)
                     /locus_tag="VN496_16515"
     CDS             complement(4761..6029)
                     /locus_tag="VN496_16515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXF18612.1"
                     /translation="MSEQAQARMRAPDIEQLLQENQELRRRAEEAEEILRALRSGEAD
                     AILVTSDREQVYVLGTADDSYRLMIEHLPYSAATLTVEGAVIQINTSFVDMLKLPPES
                     LVGKPIGDFIAPDSVSDMRALMSEGLQGNAKREIIFQRGDGALVPVYLCAKLTKEGAR
                     GAGLVITDLTEQRHYERLLETQEALRASEERLRSADRKKDEFIATLAHELRNPLGAIT
                     YAAALIGKSGITDSMVKCGSDVIERQVGHMTRLLNDLLDVGRISTGKIKLDMRPIGLA
                     SVLDAALEASRPIIERYHHSFEVISPSEPVLLTGDAERLAQVFTNLLNNSAKYTDPDG
                     NLCLTATKQAQEVIVSVRDDGVGIAPEKLPHVFDLFYQAEEGLERTSDGLGIGLSLAK
                     RLVEMHGGTIEAFSDGLGRGSEFVVRLPAS"
     gene            complement(6032..6379)
                     /locus_tag="VN496_16520"
     CDS             complement(6032..6379)
                     /locus_tag="VN496_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HXF18613.1"
                     /translation="MTAKKQQLPQTLQELAAATTVDRDVWDLRLYVAGKTAKSQAALQ
                     NLQKLCEENLPGKYRIEVVDLLVYPQLAQGDQIIAIPTLVRKLPEPIRKVIGDLSNAE
                     RTLVGLQLRPAKN"
     gene            complement(6376..8106)
                     /gene="kaiC"
                     /locus_tag="VN496_16525"
     CDS             complement(6376..8106)
                     /gene="kaiC"
                     /locus_tag="VN496_16525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HXF18614.1"
                     /translation="MKRSLPKAPTGIEGLDEITGGGLPRGRPTLICGSAGSGKTLFGV
                     EFLVRGATQFDEPGVFLAFEETADDLAQNVRSLGFDLEGLQKQKKLAVDYVHVERSEI
                     EEAGEYDLSALFIRLGHAIDSIGAKRVVLDTLETLFGGLDNTAVLRAELRRLFRWLKD
                     KGVSAIITGERGDGTLTRQGLEEYVSDCVILLDQRVNDQLSTRRLRIIKYRGTVHGTN
                     EYPFLIDESGISVLPITSMGLQHKASDERISTGIARLDAMLGGAGVYRGSTVLVTGSA
                     GTGKTSLAAHFADATCRRGERCLYFGFEESQSQFVRNMRSIGLDMEHWLKKNLLMFHA
                     TRPDAYGLEMHLAVLHKLVNKFAPHTVVIDPITTFANVGSSREAESMLIRLIDFLKSR
                     QITAFFTSLTRGGDALEQSGSGISSIIDTWMLLRDIELGGERNRGLYVLKSRGMAHSN
                     QIREFLLTPNGVELTDVYVGPDGVLTGSMRLAQEAREQAVAMTRQQEIERRQREIERK
                     RRSVEAQTAALRGQFEAEQEELMRLIEQEQAAADTLRKERGAMAASRQADDSGDGEPG
                     IPLKRTGRRA"
     gene            complement(8242..8628)
                     /locus_tag="VN496_16530"
     CDS             complement(8242..8628)
                     /locus_tag="VN496_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18615.1"
                     /translation="MLSILSRMPGEAVMKADFILWLILCALPQAVVAQQVSIAGGSTE
                     CALWVESRAAHDSEVLEHFVIGCLNGLSLGTGKEFWQANAVPISRQAVFTAMDDYCRT
                     NPAGMIVAGSIQLFKERTRPPPANFQ"
     gene            9079..9324
                     /locus_tag="VN496_16535"
     CDS             9079..9324
                     /locus_tag="VN496_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18616.1"
                     /translation="MQEKKLEIGDYVIAVKALYSPDIRKWQPILRISRRMPGSGESVH
                     QDFTLLPALSKSEADAIEYGLTKGRLLVTGTVMGLTI"
     gene            complement(9512..>9996)
                     /locus_tag="VN496_16540"
     CDS             complement(9512..>9996)
                     /locus_tag="VN496_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18617.1"
                     /translation="VHIVSHGFAGMFFANHSLALDFSLVHQPGGFIMSRLLSIAIAAA
                     LGSAISAPLYAADTVQSSTNATTHTAAMSDTQASEALDKCKNMTGTEGARCVVNIRPA
                     GGGGSSRAMAVMEDDENAVRTGMFTDEEYAAAVKHCDNGKPKSRDRCIANVKDSFGRM
                     "
BASE COUNT         2057 a         2993 c         2714 g         2232 t
ORIGIN      
        1 tgacctccgc ggtggcgtgc atcgtggatt tcaagtttac gccgcgccgg tttacgccag
       61 gctacgaacg gcgtttgtcg aaacgatcgc ttgttctcgt ctacagcgcc ttcgcgatcg
      121 gcatggcgac cgctggcctg ctgaaagacc gcaatcacca ggaataaaag ccgcgtgccg
      181 caatgctgcc gacaaggcga acgatgatac caacctgccc cgcttgcgtg ccgtggtccg
      241 atcggacttc ggtgtgttgc tgtagccact gaccaaatca gttcacccaa tgcgaactag
      301 cggatcggcg caacatcgaa ttgtcagcgc tccgcgtagc gagcaggcaa tgtcacaaca
      361 tcttccagca gcggctgaac cgcacaccag cgacgcaccg ttccggttta tcgcccggcg
      421 caacaattcg tttccgttga ccgccaacct cgccttgttc ggctccatcg ccgccgtcat
      481 gctggtcatc gcgctgggct ttgcgatggc gggcgcgtgg ccgatatttt tattttccgg
      541 actggaactg acgctggtgt atctggcgat tcggcatatg agcaagcacg cacaggacta
      601 cgaatccatc tcggttcatg acgacaagct tgtggtcgtt attaagagca aggacatgca
      661 acgctgcgtg gaattcaacc cgtattgggt ccaggtcgtg ggcggggcca gtcacggcgt
      721 gcgcgacggc gcgctgatcc tgcgatccca cggcaaagac gtggcattcg gcgtgcatct
      781 tacgggcgag cagcgtgtcg aacttgcgca ccggctgaaa caccaattgc gcatcaggcg
      841 gcatcccgac tgcgaacgcg cacaacttac tccttgaggt caagaaatga gtgcagtcat
      901 cgagcatggt catggagatg cgcacgcggg ctatccgggc ggcatcctgc ggtgggtcaa
      961 gaccacgaac cacaaggaca tcggcaccat gtatctgtgg ttcagttttg tcatgttcat
     1021 cgtcggcggc accatggccg tgctgatccg cgccgagctg ttccagccgg gcttgcagtt
     1081 cctcgaccct cagttctaca acgagatggt atcaacgcac ggtctggtga tgatcttcgg
     1141 cgccatcatg ccggcattcg tgggtttcgc gaattggctc atcccgatac agatcggcgc
     1201 ttccgacatg gcgttcccgc gcctgaacaa ctggagtttc tggctgctga ttccgggctc
     1261 cttgctattg accggcgcgc tgttcgcgcc cggcggcggc aacggcgcgg gctggaccat
     1321 gtacccgccg ctcagcgtgc agcatgggat gggcatggac atgacaattt ttgccattca
     1381 catcctgggc atgtcttcca tcatggggtc gatcaatatc attaccacca ttctgaattt
     1441 gcgcgcaccc ggcatgacgc tgatgaaaat gccgctgttc gtctggacct ggttgatcac
     1501 cgcatatctg ctcgtagccg tcatgcccgt attggcgggt gccgtgacca tgaccttgac
     1561 ggaccggcat ttcggaactc acttcttcaa tgcgcccggc gggggtgatc cgatcatgtt
     1621 ccagcacttg ttctggttct tcggccaccc tgaggtttat atcatcgcca ttccggcatt
     1681 cggcgtgatc tcacaggtga ttccggcatt ctcccgcaag ccgctgttcg gctacacctc
     1741 catggtctat gcggtgtccg ctatcgcgat cctttctttc ctggtatggg cgcatcacat
     1801 gttcaccacc gggctgccgg ttaccggcca gctctactat atgtatgcga ccatgctgat
     1861 cgccgttccc accggtgtga aagtattcaa ctgggtatcc acaatgtgga agggttctct
     1921 cagtttcgag acgcccatgc tgttcgcgat cggtttcttg ctgctgttta ccatcggcgg
     1981 tttcagcggg ttggtgttag ctatcgtacc gatcgatatc caggtacacg atacctatta
     2041 cgtggtcgcg cattttcatt atgtgatggt ggccggcgct ttgttttcgg ccttcgccgg
     2101 gatctatttc tggctgccga aatggaccgg ccatatgtac aacgaagtgc ttggcaagtg
     2161 gcatttctgg ctgtcgttta tttttttcaa catgattttt ttcgtgcagc atttcctcgg
     2221 cctggccggt atgccgcgcc gcattgctga ctatgcgcca cagttcaccg acttcaacgt
     2281 catctcatcg ctcggcgcgt ttgggttcgg cttgtcgcaa ctgctgttcc tgtacgtggt
     2341 tgtgaactgc attcgccgcg gtgaaccggc gccacagaag ccctgggagg gtgctgattc
     2401 actggagtgg acccatttgc cgacgccggc gccttatcac tctttcgaaa ctccgccgtt
     2461 gctaaggtag gacgaggctg cggtattggt cgggccggcc ggcatggcga ccgcacgaac
     2521 ggcatcgatc agcacctgta caccgtcggc cagcgtttca tgcgcggtgt cattcagcag
     2581 caccagcatg gattcccgca gcgggataat tccgtaccgg cttcggtaat gcgcaacgtg
     2641 cagtgccagc acgcgtgcca ctgcgatcat ttcccttttg ctcgcttcat cgaccaaccg
     2701 gtccgacaac tcgacaaggg cggcactgcg ttccgattcc tgggccaatg gcatcctcct
     2761 tgcgctcgcg ccgacgtaac accattgacc aaggcgagcg ctggattatc cagtcgggct
     2821 cgaattatcc ttgcttacga tcctggggag tatcaccaat gcaatatcct ttctgcgcat
     2881 ggcgcgcttc actgattcgc cattcctgca ggacgtagac atagccgcgc gatgtctcaa
     2941 gctcctccag gcctctcagc gaaaaactga gcactgtcat cccggtcagg cgcggctcga
     3001 ggatgggctt cagcagatga cccatgggcg tcgtcaaccc ggctattcgc ctgagcggcc
     3061 ccccggcctc ccgggcagct tcataaatca tgagttcgcc aaccagcgcc gccttcgcat
     3121 cgaactcttt gtcggtatag cgccttccgc gctcgtatgc agggaccacg gcaaaaagca
     3181 tgactggatg gatatacagt tgttgcccgg gattctactg ccatcgattc accgctgaca
     3241 agcacagtgg acggaatgta aaacctcctg ctttgtgaaa aaaatacacg ttcggcgttc
     3301 cgcccctgca attcgcgcgg gcagtccggc agccagtgtg agctaaacgg ccttccgcga
     3361 cggctgcaag tcgctggcat gagcattgcg gtactcggac tacacgcccg cctgcaaggc
     3421 gcagggtcgg cccgatggtt tggccttgaa acttgcaagc gcctattgaa tttaacgcag
     3481 cacgctttat tcaggagacc ggaatgaaca agttgttttt aaccttattg gccagcgccg
     3541 cgacggcgac gctagcggtt ggaagcgcga cgcacgctgc agacagcagc agctcgagca
     3601 gcggtcaaaa ctcgaaccaa ggtacgggac aaagccaggg tgcggatcaa tcttccactg
     3661 caggaaacga cagcacgtcc agcagtggtg catcgagcag caacgaatcg acgccggccg
     3721 cgcgaagaaa tccgtcgtca gcaaatgaca tgtcaaacaa aacccccggc gaaccagcag
     3781 acaaggcaac gccggacgac actggccgga ccgcgccgca aagcccgcgg gagcctagtg
     3841 ccgccggcac ggcacgcgac gatcaatcca acaataccaa tagcagcagc ggcggcgacg
     3901 caaattcgtc gtccggcgcg acttcatcga acaccgaccg cagaagtgaa gcgactacta
     3961 ctccggaccg gccatccaac cggtaagcgg atccctgttc cggtttccat agcgaagcat
     4021 caataaccca ggagaactta aaatgaagca gcatctttat gttatttcag cagctgtagt
     4081 ggcaacgatt tttggcctgg cggcgtgcac ggatcgcaac aaccaggcga gccagcccgg
     4141 atcatcgagc gccggcaagt cggcatcttc gaactcgtcc agcccatccg gatctgcggc
     4201 gaactcgtcc agcccatccg gatctgcggc aaattcctcc ggatcgacct caagcccatc
     4261 cgggagcact ggagcgaaca gcggcagccc cgctgggaac agcagttcgg gtagcagcac
     4321 aagtggaccc acggccgatt ccgggacgag ttcttcatcc agcagcaaaa agagcacgca
     4381 atagatcttt ctttgctcgc acccacgtcg ccgcccgcta cccgggcggc gacgtgattg
     4441 tttcctcgaa acgttttatt gtcatcgcaa agtggcggtc ttggacagtc gtttcacgga
     4501 cgtgcaacgg ttttacccag atagtcccga actttgctgg cctcgttgcc gaccgattta
     4561 atcgcagccc tgagctgtgc ttccgtgacc gcgagggacg tagtccagta gtgcagttgt
     4621 gcaggatcgt tgaggtcgat tttctggctc cgatcatcct gattgcgctc gttcatggtt
     4681 tctcctgaga agccctatga atcgaacgac aatgccgcac ttgatacgtt cgtaaaaatt
     4741 caggacgccg tgaaccagaa ctacgaagct ggcagcctca ccacgaattc gctgcctctg
     4801 cccaagccat cgctaaaagc ttcgatggtt ccaccatgca tttcaaccaa ccgcttcgcc
     4861 agcgaaagtc cgatgccaag cccatcgctg gtacgttcca atccttcttc tgcctggtag
     4921 aacaagtcga atacgtgcgg caatttctct ggagcaattc caacgccatc gtctctcact
     4981 gagacgatca cttcctgcgc ctgctttgtt gcagtcaggc ataggttgcc atccgggtcg
     5041 gtatattttg cggaattgtt caacaaattc gtgaaaacct gggcaagccg ctcggcgtct
     5101 ccagtcaaca gaactggctc tgaaggagat atgacttcga aactgtggtg gtagcgttct
     5161 atgatgggcc tgctcgcttc gagcgccgca tcgagcacgc ttgcaaggcc gatggggcgc
     5221 atgtcgagct tgatttttcc ggtggatatg cgaccgacat cgagcagatc gtttagcaaa
     5281 cgggtcatat ggccgacctg cctttcgatg acatcgctgc cgcatttcac catcgaatcg
     5341 gtgatgccgc ttttgccaat gagcgctgca gcataggtaa tggcgcccag cggatttctc
     5401 aattcgtgcg ccagtgtggc gatgaattca tccttttttc gatccgcaga acgtagtcgt
     5461 tcctcactgg cgcgcaacgc ctcctgtgtc tccagcaaac gctcatagtg gcgctgctcg
     5521 gtcaggtcag taatgaccag tccggctccg cgcgcaccct ctttggtcaa ttttgcgcac
     5581 agatagaccg gcaccagcgc cccatcgcct ctttggaaaa tgatttcgcg cttcgcgtta
     5641 ccttgtagcc cttcggacat cagggcccgc atgtccgaca cgctgtctgg agcgatgaag
     5701 tcgcctatcg gcttgcccac caaggactca ggcggcagct tcagcatatc gacgaagctg
     5761 gtgttgattt gaatcaccgc accttctacc gtcagcgttg ctgcgctata tggcaagtgc
     5821 tctatcatga ggcgatagga atcgtccgcg gtgcccagga cgtacacctg ctcgcgatcg
     5881 cttgtcacca atatcgcgtc tgcttcgcca ctgcgcagtg cgcgcaatat ttcttcagct
     5941 tcttcggccc tgcgacgcag ttcttgattt tcctgcagaa gctgctcgat atcgggagca
     6001 cgcattctgg cttgagcttg ttcagacatg gtcagttttt ggccggacgc aactgcagtc
     6061 ccaccaaggt acgttcagca ttcgacaagt cgccgattac tttgcggatc ggttcaggca
     6121 gctttcgcac cagggttggg atggcgataa tttgatcacc ttgcgccagt tgcggataca
     6181 ccagtagatc caccacttcg atcctgtatt ttccgggaag gttctcttcg cacagtttct
     6241 ggagattctg cagcgctgcc tgggattttg cagtcttgcc ggccacgtac aaacgcaagt
     6301 cccacacgtc ccgatcgacg gtggtcgccg cggcaagttc ttgcagagtc tgcggaagct
     6361 gctgcttttt cgctgtcatg cgcggcgacc cgtgcgtttc aacggaatgc cgggctcgcc
     6421 atctcctgaa tcgtcggcct gccggctggc cgccatggct ccccgttctt tccgtaacgt
     6481 gtcggcagca gcctgttcct gttcaatgag ccgcatcaat tcttcctgct ccgcctcgaa
     6541 ctggccgcgc aaagcggcgg tctgggcctc caccgaccgg cgtttgcgtt ctatctcgcg
     6601 ctggcgacgc tcaatttcct gctggcgcgt catggcgacg gcctgctcgc gggcttcttg
     6661 cgctagccgc atcgagccgg tcagcacacc atcgggccct acgtacacgt ccgtgagttc
     6721 tacaccgtta ggcgtcagca gaaactcgcg aatctggttg gagtgcgcca tgccgcggga
     6781 tttgagcaca tacaacccgc ggtttcgttc accccccaac tcgatatcgc gcaggagcat
     6841 ccacgtgtcg atgatcgatg aaatacccga accgctttgt tccagcgcgt ctccaccacg
     6901 cgtcaagctc gtaaaaaatg cggtgatctg gcgactcttg agaaagtcga tcagacggat
     6961 gagcatcgat tccgcttccc tggaagagcc cacgtttgcg aatgtcgtga tagggtcgat
     7021 aacaacggtg tgcggcgcaa atttgttgac cagcttgtgc aacacggcaa ggtgcatctc
     7081 caacccatac gcgtcagggc gcgtagcgtg aaacatcaag agatttttct tcagccaatg
     7141 ttccatatcc aggccgattg aacgcatgtt gcgcacgaat tggctttgcg attcctcgaa
     7201 gccaaagtac aggcagcgtt cgccgcgccg acaggtggca tcggcaaaat gcgcggccag
     7261 gctagtcttg ccggtgccgg ccgatccggt gaccagcaca gtgctgccgc gatagacgcc
     7321 cgcgccgccg agcatggcat caagacgtgc gatccccgtg gaaatacgtt cgtcggaggc
     7381 cttgtgctgc agtcccatcg aagtaatggg cagaacggaa attccgctct catcgatcag
     7441 aaaaggatat tcgttggtac cgtgcacggt gccgcggtat ttgataatgc gcaatcggcg
     7501 cgtggacagc tgatcgttca ccctctgatc cagcagaatg acacagtcgg aaacgtactc
     7561 ttcgagccct tgtcgtgtca gcgtgccgtc cccgcgttcg ccggtgataa ttgcgctaac
     7621 ccccttatcc tttagccagc gaaacaggcg gcggagctct gcacgcaaaa cagcggtatt
     7681 gtccaggccg ccgaacagcg tctccagcgt atcaagcacg acgcgcttcg cgccgatgga
     7741 gtcaattgca tgcccgagcc ggatgaacag cgcgctgaga tcatattcgc ccgcctcttc
     7801 gatttcgctg cgctctacgt gaacataatc cactgcgagt tttttctgtt tctgcaaccc
     7861 ttcgaggtcg aagcccagcg agcggacatt ctgtgccagg tcgtcggcgg tctcttcaaa
     7921 ggccagaaat acaccgggct cgtcaaactg ggtcgcgccg cgaaccagga attcgacgcc
     7981 aaagagcgtt ttgccgctgc ccgcgcttcc gcagataagt gttgggcgcc cacgcggcaa
     8041 gccgccgcca gtaatttcgt cgagcccttc aatgccggtt ggcgccttcg gaagcgaacg
     8101 cttcacggcg ctggcattcg tttcagcttt cctcatctcc cgcccaacct tgatttccgc
     8161 tactgcattg ttagttattt gcaaaattat acgttagctt ttctgcaccc gaaacaacat
     8221 gagcagcgat gtgagaaagt gtcactggaa attggcaggc ggcggccgcg ttcgctcttt
     8281 gaacagctgg atcgatccag ctacgatcat tccagcagga ttggtccggc agtaatcatc
     8341 catggcggtg aaaacggctt gccggctgat cggcactgca ttggcctgcc agaactcttt
     8401 tccggttccc agagataacc cattgaggca accgatgaca aaatgctcca gaacttcgga
     8461 atcatgagcg gctctcgatt cgacccacag cgcacactca gtggaaccac ccgcaatgga
     8521 gacttgctgt gctactaccg cttgcggtag cgcgcacaga atcagccaca gtatgaagtc
     8581 tgccttcatt actgcctccc ctggcatcct gctcaatatg ctcagcatgg ttgcgtccag
     8641 gaacctacgc ttcacccgca aatccaaaaa ctgagccggt tgatatcaaa atagcatgag
     8701 ccatcacagc tcctgcccat gaccctgtgc cgggccttat gacaatctgg tgccacaatg
     8761 ccaaaggtac gcttgaaccg cgatgttcct cggagcaagt gacattcgga ctagccggcg
     8821 taggttactc ctttggatgg cctgccggaa actagtggtc aagtactgcc gcatggacgc
     8881 gtcgctggaa ccccgatggt gcggctgcac tgaaacggat cctggctccg ccaccgcacg
     8941 ctcacaggct gccgactgaa tacagtaagc gcgggaccga accccattcg cggcacaaca
     9001 aaattggttg ccagcctttc actaccctcg cgatgaacgg ggtagcgtaa gattcgcccc
     9061 ctaaggaggc agggcgccat gcaggagaaa aaactcgaaa tcggcgatta cgtgatcgcg
     9121 gtgaaagcgt tgtactcacc cgacatacga aaatggcaac cgatattgcg tatttcccgc
     9181 cgcatgccgg ggtccggaga atcggtccat caggatttca ctctgctacc ggcgctttcg
     9241 aaatcggagg ccgacgccat tgaatatggg ctgaccaaag gacgattgct ggtcacggga
     9301 acggtgatgg gcttgacgat ttgacgcaca aatttccgct ccaatcgctg tacggccact
     9361 gaaataacga aacccgggag gtgggtgcct cgcgggtttc gcacgccgcg ccaaccgaac
     9421 tgattcgagg gcgacgcaac cttctacagg tatggagcgt tagtcaaacg ctgacgacaa
     9481 tagttccttg ttccgttcgc gaaccaccgg gttacatgcg accgaagcta tctttcacgt
     9541 tggcgatgca gcggtcccga cttttaggtt tgccgttgtc gcagtgcttc accgcggccg
     9601 cgtattcctc gtcggtaaac attccagtcc tcaccgcgtt ctcatcatct tccatcaccg
     9661 ccatcgcgcg cgaactgccg ccgccaccag cgggccgtat gttgacgacg caccgcgcac
     9721 cctcggttcc cgtcatattt ttgcacttgt ccagtgcctc gctcgcttgc gtatcagaca
     9781 tggcggcagt gtgagtagtc gcgtttgtag aggactgcac cgtgtcggct gcatatagag
     9841 gcgcgctgat cgcagatcca agtgcggctg caatcgcaat gctaagtaat ctcgacataa
     9901 taaaacctcc aggttgatga acgagtgaaa agtctagtgc cagagaatga tttgcaaaga
     9961 acatgcccgc aaaaccatgc gagacaatgt gcaccc
//
LOCUS       DATMZQ010000519        10693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1636870, whole genome shotgun sequence.
ACCESSION   DATMZQ010000519 DATMZQ010000000
VERSION     DATMZQ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636870"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VN496_16545"
     CDS             <1..401
                     /locus_tag="VN496_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HXF18618.1"
                     /translation="PKIALLSHSNFGSSRNPSAQKMSKAREILEQRAPQLEVDGEMHG
                     DAALDEKIRLRIFPNSRLKGEANLLIMPTVDAANITFNLIKTISGEGITVGPILLGAA
                     KPVHILTPTATVRRLVNMAALASVDASFSR"
     gene            454..1602
                     /locus_tag="VN496_16550"
     CDS             454..1602
                     /locus_tag="VN496_16550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXF18619.1"
                     /translation="MTLPVLFEDLVAGNGARIGVARLNAERTLNALSLEMIDLLAQRL
                     AFWAADPGVAIVVLEGTGDKAFSAGADLHKMHATMIAHHASPQRDDIRANPYAAAFFG
                     REYRLDYLIHTYPKPILCWGHGIVMGGGVGLMSGASHRVLTHESRVAMPEITIGLFPD
                     VGGSWLLERMPGATGLFLALTGARLQASDALFVKLADYCIGQSNKATVFERLMTQPWV
                     QASAANRRLLSALLQEFATPALPSGPLRQHFDLINQLCGSTELIDIVAALTALKTEDS
                     WLARAVTTLAAGSPSTAALSYELQRRVKYLSLADVFRLEFVAALHCASRPDFAEGIRA
                     LLIDKDQRPRWQPKTLAQISEEWLEGYFANPWTQQEHPLADLGTMPLR"
     gene            1723..2436
                     /locus_tag="VN496_16555"
     CDS             1723..2436
                     /locus_tag="VN496_16555"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXF18620.1"
                     /translation="MQAPNRRLALITGANKGIGFEICRQLARKGLRVLLTSRDPRKGV
                     KAQKILLQENLDVLFHELDVTVLSSVQALVKYVEREHGGVDALVNNAGIMIDSSATSI
                     LREREDVFHATLETNFFGALRMCQGLVPLMRKRGYGRVVNLSSGLGQLTDMGDGNAAY
                     RMSKTALNTLTRMVASAMEQQDILVNSMCPGWVRTDMGGPNASRSVEKGAETAVWLAT
                     LPGGGPTGGLFRDKKPIPW"
     gene            2448..3884
                     /gene="fumC"
                     /locus_tag="VN496_16560"
     CDS             2448..3884
                     /gene="fumC"
                     /locus_tag="VN496_16560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXF18621.1"
                     /translation="MKTRMERDSFGAIAVPAERLWGAQTQRSLQNFRISGERMPLEMI
                     YALALVKRACTRVNADLGALDPGKAQAIVKAADEIIAGKHDAEFPLVVWQTGSGTQTN
                     MNLNEVLANRASELLGGERGEKRLVHPNDDVNKGQSSNDVFPTAMHVAAATELHKRLM
                     PAVTALRDTLRSKANLFGDIVKVGRTHLQDATPLTLGQEFSGYVSQLEHGLAHVQAAL
                     DHLYELALGGTAVGTGLNAHPEFGTRVAEELSKSTNLPFLSAPNKFEALAANDALVHA
                     HGALKTLAASFMKIANDIRWLASGPRSGIGELRIPENEPGSSIMPGKVNPTQSEALTM
                     LCCQVLGNDVAVNIGGAMGNFELNVFKPLIIHNFLQSVRLLADGARSFNDNCAIGIEP
                     NLDRIERMLNQSLMLVTALNPHIGYDKAAEIAKKAYKDGTTLKEAALALQYLTAQQFD
                     EWVRPEDMVGTRSLADRSRPAGRRRRAK"
     gene            3966..4499
                     /locus_tag="VN496_16565"
     CDS             3966..4499
                     /locus_tag="VN496_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXF18622.1"
                     /translation="MKGELAKLMTQFPRAGALEWIGIRPERRAPLLALTRVEAIVGIG
                     LAGDHYASKTHGKRQVTLIQQEHLDVVANMLGKSEVLPATLRRNLVVSGINLYALRNT
                     RFRIGSVLLEGSGPCEPCSRMEEVLGTGGYNAMRGHGGITARVIEGGELCIGDRVEAA
                     SPDATPVTILSSVPEVK"
     gene            4530..5162
                     /locus_tag="VN496_16570"
     CDS             4530..5162
                     /locus_tag="VN496_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXF18623.1"
                     /translation="MPGSARFNNLPYSRAWLMERLRLKASPRPDENFPPYAHSAELEP
                     VPAAVLVPIVNQPDGLTLMFTQRTAHLYDHAGQISFPGGRVDAADADRIATALREAQE
                     ETGLCASRVQVIGQLPDYDIPTGFRVTPVVGWVEPPFELKPDAFEVADVFEVPLEFFL
                     DPANHRRHSDIRNGRTRYYYSMPYGERNIWGATAGMVYSLYQLLTADIGK"
     gene            5159..5650
                     /locus_tag="VN496_16575"
     CDS             5159..5650
                     /locus_tag="VN496_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HXF18624.1"
                     /translation="MSKDKTPGTPAVRQLRAANVQFTDHAYEYEEKGGTAVSARELGV
                     PEHAVIKTLVMEDESRTPLIILMHGDLKVSTRELARIAGVKTIVPCNPETAHRHSGYT
                     VGGTSPFGTRRKMPVYMEETVLALPKIYINGGRRGYLVGMVPVDLVRVLAPKLVKVGI
                     KES"
     gene            5801..6130
                     /locus_tag="VN496_16580"
     CDS             5801..6130
                     /locus_tag="VN496_16580"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HXF18625.1"
                     /translation="MIELAALLLFLVAGWFWLDSLRARDVALDGARRACEAEGVQLLD
                     WTVALRRIRLGRDEEGRRGFQRTYEFEFSDTGNNRIGGSITVLGRQLLSLNLPVVAPT
                     SNVIRLH"
     gene            complement(6158..6436)
                     /locus_tag="VN496_16585"
     CDS             complement(6158..6436)
                     /locus_tag="VN496_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18626.1"
                     /translation="MLNVIGLPLFAVVLGLLIVACFRLAQSRLVLITGLLWILYAVYE
                     YLVQIRVLCSGECNIRVDLLLIYPILFIYTVTSILRTGKALWQRRFKG"
     gene            6581..6853
                     /locus_tag="VN496_16590"
     CDS             6581..6853
                     /locus_tag="VN496_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18627.1"
                     /translation="MSNEQHAQEIAMLRAEVEMLMAERQALLRATGAAAVFVANLDSE
                     SLPEDTYAVADILAHALNDLSEDTLRDALELVQPSIESARAEQKEQ"
     gene            6887..8062
                     /locus_tag="VN496_16595"
     CDS             6887..8062
                     /locus_tag="VN496_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXF18628.1"
                     /translation="MKDPSSSQAKIGLILTGGGARAAYQVGFLRAVSHLLPRGTPNPF
                     KIICGTSAGAINAAALAANATDFHRGVRQLLLIWKYFHVDQVYRSDVAGVLGTGMRWL
                     LAMLMGGLGKNNPSSLLDNSPLSQLLRERLDLGGIRRSIDAGALYALSVSVSGYASGQ
                     SVNFFQSVPGVAGWQRARRIGIPEIIDFEHLLASSALPFIFPAVPLRREFFGDGSMRQ
                     IAPISPALHLGADRVLVIGVGRQMQNPPPRMKMASYPSLAQIAGHALNSIFLDGLEVD
                     IERLERINKTIQLIPREERNKHNMQLREVDVLVITPSEEIDRIAAKYFHALPRSIRFL
                     LRGLGATRTGGATLTSYLLFEQEYCRALIALGYKDTMARRDDILRFISPGAWSARGQ"
     gene            8635..9822
                     /gene="urtA"
                     /locus_tag="VN496_16600"
     CDS             8635..9822
                     /gene="urtA"
                     /locus_tag="VN496_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HXF18629.1"
                     /translation="MSKFDRRDFLKAGAATAAAGVLPAPFVMKAAHAADPIKVGILHS
                     LSGTIAIAEAAIVDAEKMAIDEINAAGGVMGRKIEPIVEDGASDWPTFAEKARKLIQK
                     DKVAAVFGCYTSASRKAVLPVFEQNNGLLYYPTYYEGQEESKNVIYTAQEATQSVIAA
                     VEWLASNKGKTFFLVGSDYIYPRTCNKIAKPTITRVGGKTLGEEYAPLGHTEFSSIIN
                     KIKAAKPDCIYSTVVGGSNVAFFKQLKAAGLSGDKQTLLSTVVSENEIDGIGKENAVG
                     YYACMGYFQSQKNPANEKFVKAIKAKYGQDRVVGDPMECGYTGVYFWKLAVEKAKSLD
                     VQKVVAASSNLPFAAPEGDIKIHATNHHTWKHARIGRARADGQFDIVHESGLLEPNPF
                     PKL"
     gene            9948..>10693
                     /gene="urtB"
                     /locus_tag="VN496_16605"
     CDS             9948..>10693
                     /gene="urtB"
                     /locus_tag="VN496_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HXF18630.1"
                     /translation="MSLDIAVMQVFSGISLFTILVLMALGLAIIFGLMGVINMAHGEL
                     MALGAYVTYMTAHVFEKFMPSLMGVYLFFAIAFSFIVTFVFGWILERGFIRFLYNRPL
                     DTMLATWGLSLVLQQTYRSVFGAQEVSVPMTSWLEGAWEPVVGIQLPLNRLFIVGLTI
                     IVATLLYLALFKTRWGLKTRAVTQNRLMSGAAGINTDKVDAATFAVGSGLAGIAGSVF
                     TMVGSTNPLTGQLYIVDSFIVVVFGGVQTLL"
BASE COUNT         2296 a         2964 c         3155 g         2278 t
ORIGIN      
        1 cgccgaagat tgcactgctt tcgcattcca atttcggcag ctcgcgcaat ccgtctgcac
       61 aaaaaatgag caaagcgcgt gaaattttgg aacaacgtgc gccgcaactg gaagttgatg
      121 gcgagatgca tggcgatgcg gcgcttgacg aaaaaatccg gctcaggata tttccaaact
      181 cccggctgaa gggcgaagcc aatttgctga tcatgccgac ggtcgatgca gcaaacatta
      241 ccttcaacct gatcaaaacc atctccggcg agggcattac tgtcgggccc atcctgctcg
      301 gcgccgcaaa gccggtgcat atccttaccc cgaccgctac ggtacgcagg ctggtgaaca
      361 tggctgcgct ggcttcagtc gacgcatctt tcagccgctg atatccgcat gccgatcgca
      421 cgatggctcc ctgcgacgag ttcgaaaatt gacatgaccc tgcccgtcct gttcgaagat
      481 ttggtcgctg gcaatggcgc acgcatcggc gttgcccggc tcaatgccga gcgcacgctg
      541 aatgcattgt cgctggagat gattgatctg ctggcgcagc gcctggcttt ctgggccgcc
      601 gatccgggcg tcgcaatcgt ggtgctcgaa ggcaccggag acaaggcatt ttctgccggc
      661 gccgacttgc acaaaatgca tgcgaccatg atcgcgcatc atgcttcgcc ccagcgcgac
      721 gacatccgcg ccaatcctta tgcggctgct tttttcggac gcgaatatcg gctggattat
      781 ctgattcata cctatcccaa accgatctta tgctggggcc acggcattgt catgggcggc
      841 ggggtggggt tgatgtccgg tgcaagccat cgagtgctaa cccacgaatc gcgcgtggcc
      901 atgcccgaaa tcaccatcgg actatttccc gatgtgggcg gcagctggtt gctggagcgc
      961 atgccaggcg caacggggct ttttctggcg ttgaccggtg cgagattgca ggcatccgat
     1021 gcgctgttcg tcaaactcgc agactattgc atcggccaat caaacaaggc gacggtattc
     1081 gagcgcctga tgacgcagcc gtgggtacag gcgtccgcgg caaaccgtcg cctgctgtcg
     1141 gcactgctgc aggagttcgc aacaccggca ttgcccagtg gaccattgcg ccagcacttc
     1201 gatttgatca accaactgtg cggcagcacg gagttgatcg acattgtcgc cgcccttacc
     1261 gccttgaaga ccgaagacag ctggcttgcg cgtgcagtga ctacgctggc cgcaggctcg
     1321 cccagcaccg cggcgttgag ctacgaattg cagcggcggg tcaaatatct atcgctcgcc
     1381 gatgtatttc gcctcgagtt tgtggcggcg cttcactgcg caagccggcc cgactttgcc
     1441 gaaggtatcc gcgcgctgct gatcgacaag gaccagcgcc cgcgctggca accgaagacg
     1501 ctggcacaga tttccgaaga atggctcgaa gggtatttcg ccaacccctg gacacaacag
     1561 gagcatccgc tggctgatct tggcaccatg ccgttgcggt gagcggacgc gcgccagaaa
     1621 cttaatttcc gccgtgcagg tcacacaaca cggggcggcg ttccttttgc tcatcccgct
     1681 tgaataatct ttgcaggccg aggaaccgga tcgagaacgt gcatgcaagc accgaatagg
     1741 cgactggcct tgatcaccgg ggccaataaa ggcatcggct tcgagatatg caggcagctt
     1801 gcgcgcaagg gactgcgcgt gctcttgacc agccgcgacc cgcggaaagg tgtaaaggcg
     1861 caaaagatcc ttttgcagga aaatctcgat gttctgtttc acgaactgga tgttaccgta
     1921 ctcagtagcg tgcaggccct cgtaaagtat gtggaaagag agcatggtgg tgtggatgcg
     1981 ctggtgaaca acgctggcat catgatcgac agttcggcaa ccagcattct gagagaacgt
     2041 gaagatgtat ttcatgccac gctggaaacc aatttctttg gcgccttgcg catgtgtcag
     2101 gggctcgtgc cgctgatgcg caagcgcggc tatgggcgcg tggtaaatct ttccagcggc
     2161 ctcggccagc tcaccgatat gggcgacggc aacgcggcgt accggatgtc caagactgca
     2221 ctgaacacgc tgacccgcat ggtggcgagc gcaatggagc aacaagatat cctggtgaat
     2281 tcaatgtgcc ccggctgggt gcgcactgac atgggcggtc cgaacgccag tcgcagcgtt
     2341 gaaaagggcg ccgaaacggc ggtctggctc gcgacattgc ctggcggcgg tccgacgggc
     2401 ggattgttcc gagataaaaa acccattccc tggtgaaccg gcgtcaaatg aaaaccagaa
     2461 tggaacgaga cagtttcggc gcgatcgcgg tgccggccga aaggctgtgg ggcgcgcaga
     2521 cacaacgcag cctgcagaat ttccgcatat caggagagcg catgccgctg gaaatgattt
     2581 acgcgcttgc gctggtaaag cgtgcctgca cgcgcgtgaa tgcggacttg ggagcgctgg
     2641 accccgggaa agcgcaggcc atcgtcaaag cagccgatga aataatcgcc ggcaagcatg
     2701 acgcggaatt tcccctcgtg gtctggcaga cgggctccgg tacccagacc aacatgaacc
     2761 tcaatgaagt gctggcaaat cgcgcgtccg aactcctcgg cggcgaacgc ggcgaaaagc
     2821 gcctggtgca tccaaatgac gatgtcaaca aaggtcagtc gtcgaacgat gtctttccca
     2881 ccgccatgca cgtcgcggca gccaccgaac tgcacaagcg attgatgccg gctgtaacag
     2941 ctttgcggga taccctgcgc agcaaggcaa acctctttgg cgacatcgtc aaggttggcc
     3001 gcactcacct gcaggacgca acgccgctta cgctcggcca ggagttttcc ggctatgtct
     3061 cgcaactcga gcatggcctg gcacatgtgc aggcggcgct tgatcacctg tacgaactcg
     3121 cactgggcgg caccgcagtg ggcaccggac tcaatgccca tccggaattc ggcacacggg
     3181 tggcggaaga gttgtcgaag tccactaact tgccgttctt gtccgcgccc aacaaattcg
     3241 aagcgctcgc tgcgaatgat gcgctggtgc acgcgcacgg cgcactgaag acgttggcgg
     3301 catcgttcat gaagatcgcg aacgatatcc gttggctcgc atccggacca cgcagcggta
     3361 tcggtgaact tcgcattccc gagaacgagc ccggcagttc gatcatgcca ggaaaagtaa
     3421 atccgacgca gtcggaagcg ttgaccatgc tttgctgcca ggtgctcggt aacgacgttg
     3481 ctgtcaatat cggtggtgcc atgggcaact tcgagttaaa cgtattcaaa ccgttaatca
     3541 tccataattt tctgcaaagc gtgcgattgc ttgccgatgg tgccaggagc ttcaacgaca
     3601 attgtgccat cggtattgaa ccgaacctgg atcgcattga acggatgctg aaccagtcac
     3661 tgatgctggt gactgcgctt aacccgcata tcggctacga caaagctgcc gaaattgcga
     3721 agaaagccta caaggacggt acgaccttga aggaggcggc gttggcactg caatacctga
     3781 cggcacagca attcgatgag tgggttcgtc ccgaagatat ggtcgggaca cgcagcctgg
     3841 ccgaccgctc tcgccctgct gggcggagaa ggcgcgcaaa atagcgttgt catttctgcg
     3901 gtgaacccgg aacagattat aaaagccacg caccgagcgg ttcagccgaa gaaggcgggg
     3961 acgaggtgaa aggcgagctt gccaaactga tgacgcaatt tccgcgcgca ggggcgctgg
     4021 agtggattgg catacggccg gagcgccgcg cgccgctctt ggcgctgaca cgcgtggagg
     4081 cgatcgttgg catcggcctg gctggcgatc actatgcgtc gaagacccat ggcaaacgcc
     4141 aggtcacctt gatccagcaa gagcatctcg acgtcgtcgc gaacatgctg ggtaaatcgg
     4201 aagtgctgcc ggcaaccttg cgccgcaatc ttgtggtatc tgggatcaac ctttacgccc
     4261 tgcgcaacac gcgatttcgt atcggcagcg ttctgctcga gggtagcggg ccttgcgaac
     4321 cctgttcacg catggaagaa gtgctgggga ccgggggcta caatgccatg cgcgggcacg
     4381 gcggcattac tgcgcgcgtc atagagggcg gcgagctttg catcggcgat cgcgtcgagg
     4441 cggcttcacc ggatgcgacc ccggtaacaa ttttgtcatc ggtgccggag gtaaaatagc
     4501 ggctggtata aacgcacctg ccgccttcag tgcctggatc agcccgcttc aacaatttgc
     4561 cgtacagccg tgcatggctg atggaacgct tgcgccttaa ggcgagtccc cgccctgacg
     4621 aaaattttcc gccttacgcc cattccgcgg aacttgagcc ggttcctgca gcggtactcg
     4681 ttcccattgt caatcagcca gacggtttga cactgatgtt cacccagcgt accgcccatc
     4741 tgtatgatca tgctggccag atcagttttc ctggcggacg ggtcgatgcg gcggatgccg
     4801 atcgcattgc caccgcgctg cgcgaagcgc aagaagaaac cggcctttgc gcttcccgcg
     4861 tgcaagtgat cgggcaactt ccggattacg acatcccaac cgggtttcgc gtcacgccgg
     4921 tggtgggctg ggtcgagccg ccattcgaac tgaagcccga tgcctttgaa gtggccgacg
     4981 tgtttgaagt gcccttggag tttttcctcg atcctgccaa tcatcggcgg cacagcgaca
     5041 ttcgcaacgg gcgcacgcgt tattactatt cgatgccgta tggtgagcgc aatatctggg
     5101 gcgctacggc cggcatggta tacagcctgt accagctatt gactgccgat attggcaaat
     5161 gagcaaggac aagacgccag gcactcctgc cgtgcgtcaa ttgcgtgccg cgaacgttca
     5221 gtttaccgat catgcgtacg aatacgagga aaaaggcggg acagccgtat cggcgcggga
     5281 gcttggtgtt cctgaacatg ccgtaatcaa aaccctcgtc atggaagacg agtcgcggac
     5341 tccgctgatt atcctgatgc atggcgattt gaaagtatcg accagggaac tggcaagaat
     5401 cgctggcgtg aagaccatcg tcccttgcaa tcccgagacg gcgcaccgac attccggtta
     5461 cacggtcggc ggtacctctc cgttcggcac tcgccggaag atgccggtgt atatggaaga
     5521 gacagtcctc gccttgccaa aaatctacat taacggtggc aggcgcggct acctcgtcgg
     5581 tatggttcct gtggatttgg tacgagtgct tgcaccaaaa ttggtgaagg taggaatcaa
     5641 agagtcgtga agcttttacg acgtgatgac gcgaaaatgg gatcagacac gcgacatgac
     5701 taaccatccg gcgatccgac tattccacca gtgatgtgtc tccagctttg cttttttacc
     5761 tcgccacgcg tcacgactgt cacgccggct cggctttgtc atgattgaac tggccgcgct
     5821 gttgttgttt ctggttgccg ggtggttctg gcttgatagt ctgcgcgcgc gcgatgtggc
     5881 gcttgacggc gcccgtcgag cgtgcgaagc cgaaggcgtt caactgctcg actggacggt
     5941 cgcactgaga cgcattcggc ttggccgcga tgaggagggg cgcaggggat ttcagcgcac
     6001 gtacgaattc gaattcagtg acacgggtaa taaccgcatt ggcggatcca ttaccgtact
     6061 gggcaggcag ttgctttcac tgaacttgcc ggtcgtggcc cccacttcca atgtcatcag
     6121 gctgcattga tgacagagcg cggacacgga tcagtggtca gcctttgaat cgtcgttgcc
     6181 acaacgcctt tccggtacgc aaaatggaag tcacggtata gatgaacaga attggataaa
     6241 ttaacagaag atccacgcgg atgttgcatt ctccgctgca caacacgcga atctgcacca
     6301 gatattcata caccgcatac aaaatccata gcaagccggt aatcagcaca agccggcttt
     6361 gcgccagccg gaaacaggcg acgatgagca agcccaggac aaccgcgaac aaaggcagac
     6421 caataacgtt gagcaagtgt cctcccgctc acattgcagc ttgcacttgc agcgccccgc
     6481 tcgaaccaaa cccggcatca accgaggtgt cctaaaggct gctggctctg gcaaaagcct
     6541 tgtggcatca tgattttaac aacaagagcc ttggaggcgt atgagcaacg aacagcacgc
     6601 gcaggaaatc gccatgctgc gcgcggaagt cgagatgctg atggccgaac ggcaggcgct
     6661 gctgcgcgca accggcgctg cggcagtgtt cgttgccaac ctcgatagtg aatcgttgcc
     6721 agaagatact tacgcggtgg ccgacattct tgcccacgca ctgaatgact tatcggaaga
     6781 cacgctgcgc gatgcgctcg agctcgtcca gccttctatt gaatcggcgc gcgctgagca
     6841 aaaagagcaa taagagcgcc gtgctcgtgc gggctgcgag gacggtttga aagatccgtc
     6901 atcttcccaa gcgaagatag gcttgatcct cactggcggc ggcgcacggg ctgcttatca
     6961 ggtcgggttt ctacgggcgg tttcgcattt gctgccgcgc ggaaccccca accctttcaa
     7021 aattatttgc ggtacttcgg ctggcgcgat caatgcggcg gcactggccg cgaacgccac
     7081 ggattttcat cgcggcgttc gccagttgct gctgatatgg aaatatttcc atgttgacca
     7141 ggtgtatcgc tcggacgtgg cgggggtgct cggtacgggc atgcgctggc tgctggccat
     7201 gttgatgggc ggtctcggca aaaataatcc cagctccttg ctggacaatt cgccactatc
     7261 tcaattgttg cgagagcgtc tcgacctcgg cggtatccgc cgcagcatcg acgccggagc
     7321 gctttacgcc ttgtcggtca gcgtatcagg gtatgcatca ggtcagtcag tgaatttttt
     7381 ccagagtgtt ccgggagtgg ccggttggca gcgtgcacgc cggattggca taccggagat
     7441 catcgatttc gagcacttgc ttgcctccag tgcgctgccc tttatttttc cggccgttcc
     7501 gctgaggcgt gagtttttcg gtgacggcag catgcgccag atcgccccca tcagcccggc
     7561 actgcacctt ggcgcggatc gcgttctggt tatcggggtc ggtcggcaga tgcaaaatcc
     7621 accgccgcgc atgaaaatgg ccagctatcc atcgctcgcc cagatcgccg gtcacgcgct
     7681 taacagtatt tttctcgatg gcctggaagt cgacattgag cgcctggagc gtatcaacaa
     7741 gaccatccag ctgattcctc gggaggaacg taacaagcac aacatgcagc tgcgggaagt
     7801 cgacgtgctg gtgatcacgc caagcgagga aattgatcga atcgccgcga agtatttcca
     7861 cgcgttgcca cgctccatcc ggtttctgct gcgcggactc ggcgcaacgc gtacgggcgg
     7921 ggctacgctg accagttatt tgctgttcga gcaggaatat tgccgggccc tgatcgcgct
     7981 gggctacaag gacacaatgg cacggcgcga cgatattttg cgttttatta gcccgggggc
     8041 atggtccgca cgcggacaat agtagccgag ggtcgttgta ggacctgcat gcggcgcccg
     8101 gccgtaatcg cacccgtcat gtcgcgtcgt actgaatgac atcggtttcg tcgctatatg
     8161 cgaggcttga atatgaagtg cgtaaggatg tataaagaag ggcggggagg cggccctgtt
     8221 aagccgctct gcatgagggc cgagccaggg cgcgtggacc gccaatgcac gggtcccgtg
     8281 cagtggtcgt atttggtgcg gtgcgcgctc gatgaattta tgccgcgaat agtgcttgat
     8341 catgttgtgg cgaagagata accatcggct ctcaaactgt ttccccttgt tgattgagtg
     8401 gctcagtggg tgcttcgcta tcagtcttca gtgtccctgt tggctcgcat tttgcgatgc
     8461 aaccaggtaa aaatggcgaa gagccagcgg cgtctgcggt aactggattt tttcggatgc
     8521 gaccggattg tctcatccga gcctggtaga gagcccatag gccgaacgcg gctcatgggc
     8581 tttttttgat tgccgcaatt cgcggtactg ttttcacggg agaggagtta actgatgagc
     8641 aagttcgaca ggcgggattt cttgaaggcc ggtgccgcca cggctgcagc gggtgtattg
     8701 ccggcgccct ttgtaatgaa ggccgctcac gcggccgatc ccataaaggt cggtatcctg
     8761 cattcgctga gcggcacgat agcgatagcg gaggctgcaa tcgtcgacgc tgagaaaatg
     8821 gcgatcgacg aaatcaatgc agccggtggt gtgatgggcc gcaagatcga accgattgtg
     8881 gaagacggtg ccagcgactg gcccaccttc gccgagaaag cgcgcaagct aatccagaaa
     8941 gacaaagtag cggccgtttt cggttgctac acgtccgctt ctcgtaaggc tgtattgccg
     9001 gtgttcgagc aaaacaacgg gttactctat tacccgactt attacgaagg tcaggaagaa
     9061 tcgaagaacg tcatctacac cgctcaggaa gcaacgcagt ccgtgattgc ggcagtcgaa
     9121 tggctggcca gcaacaaggg caagacgttc ttcttggtgg ggtccgacta tatctacccg
     9181 cgtacctgca acaaaatcgc caaacccaca atcacgcgcg taggcggcaa gacgctcggt
     9241 gaagaatacg ctcctcttgg ccatactgag ttctcgtcga ttatcaacaa gatcaaggca
     9301 gcgaagccgg attgcatata cagcaccgtc gtaggcggta gcaacgtcgc attcttcaag
     9361 caactgaagg cagctggcct ttccggcgat aaacagacgc tgctctctac cgtggtttcc
     9421 gaaaacgaga tcgacggcat cggcaaggag aacgcggtcg gttattacgc ctgcatgggc
     9481 tatttccaga gccagaaaaa cccggcgaat gaaaaattcg tcaaggccat caaggcgaaa
     9541 tatggtcagg atcgcgtcgt gggcgatccg atggaatgcg gttacacggg cgtatatttc
     9601 tggaagctcg cggtcgaaaa agcaaaaagt ctggacgtgc agaaagtggt cgcggcatcc
     9661 tcgaatcttc cgttcgcggc tcccgagggc gatatcaaga tccacgccac gaatcatcat
     9721 acctggaaac atgcacgcat cggacgtgcg cgtgcggatg ggcaattcga tatcgtgcat
     9781 gagtcgggtc tgctggagcc gaatcctttc ccgaaactgt agaaaagtcg gcgccctgat
     9841 ttacgcggac acccgctggt gatgaagcag gcagcacatt cccgcttcgt cacttgcggc
     9901 ctgcatcaat cccgtccccg ctctttgtct ttcggaaccg cccattcatg agccttgata
     9961 tcgcagtgat gcaggttttc agcggcatca gtctgtttac catcctggtg ctgatggcgc
    10021 ttggtttggc catcattttt ggattgatgg gcgtcattaa tatggcgcac ggtgaactta
    10081 tggcgttggg cgcatatgtg acttatatga cggctcacgt attcgaaaaa ttcatgccct
    10141 cgctgatggg cgtttatctt ttctttgcaa ttgcgttctc gttcatcgtc acgtttgttt
    10201 tcggctggat attggagcgg ggattcattc gatttctcta caaccggccg ctcgatacca
    10261 tgttggctac ctggggtctt agccttgtgc tgcaacagac ttaccgctca gttttcgggg
    10321 cccaggaggt tagtgtcccg atgaccagct ggcttgaggg agcgtgggaa ccggtagttg
    10381 gaattcagct gccgctgaat cgccttttca ttgtcggcct taccattatc gtcgccacct
    10441 tgctttatct tgcacttttt aaaacgcggt ggggtctgaa gacccgcgct gtgacgcaaa
    10501 acagattaat gagtggagct gcaggtatca atactgacaa ggttgatgcc gcaacattcg
    10561 cagttgggtc gggtctggca ggaattgcgg gtagcgtatt caccatggtc gggtccacca
    10621 atccgctgac cggccagctc tacatcgtcg attccttcat cgtcgtcgtg ttcggcggag
    10681 tacagaccct gct
//
LOCUS       DATMZQ010000520         5410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5096502, whole genome shotgun sequence.
ACCESSION   DATMZQ010000520 DATMZQ010000000
VERSION     DATMZQ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5096502"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..154
                     /locus_tag="VN496_16610"
     CDS             35..154
                     /locus_tag="VN496_16610"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18631.1"
                     /translation="MGVEAILRGAREMYTDDDQLLSEVEKIFNYLYGTFTAQP"
     gene            129..1490
                     /gene="chrA"
                     /locus_tag="VN496_16615"
     CDS             129..1490
                     /gene="chrA"
                     /locus_tag="VN496_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXF18632.1"
                     /translation="MAHSQPSPEAAPAGVSFRDALVYWLKLGFISFGGPAGQIAVMHQ
                     ELVERRRWISERRFLHALNFCMVLPGPEAQQLATYLGWLMHGTLGGIVAGGLFVLPSL
                     LILVALSWIYMVWESTPLFAGILYGIKPAVTAIVLFAAWRIGSRALRNAALWGISAAA
                     FVAIYFFQVPFPAIVLAAGMIGLVGGRLWPQKFAAGGGHADTGKTYGRALIDNDTPTP
                     AHARFRWNRLARVAATGALLWLLSLGAMAFARGWDDTLSQMGWFFTKAALLTFGGAYA
                     VLPYVVQGAVEQYQWLTPTQMIDGLALGETTPGPLIMVVAFVGFVGAWAHAVFGPDSV
                     VLAGIAGAAVATWFTFLPSFVFIFVGAPFIETTHRQPHLTAPLTGITAAVVGVIGNLA
                     VFFALHVFWPQGLHGGVEWPAILIATAATVALFRYKAGIIPVICACGAAGLAVQAVRA
                     LAQ"
     gene            complement(1577..2203)
                     /locus_tag="VN496_16620"
     CDS             complement(1577..2203)
                     /locus_tag="VN496_16620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF18633.1"
                     /translation="MLFSLLRRFLKLHLFRITTRPLIEQRHVTAAMRLRTVDEAELLP
                     YCSDAAIQLPISHVRAAFARGNRCAVAFDGTRLVGYEWFAHGPTPHIDGVWVAFDGNA
                     RYGYKQFVRLQYRGQRIAAALSTHADNWYRRIGCTHAISLIDLGNGASWRSERRIGAH
                     TAGYAGYIHLFGRFIAFGTPGATRLKLRFYSPRRERPNSEALRHSSYE"
     gene            2415..2780
                     /locus_tag="VN496_16625"
     CDS             2415..2780
                     /locus_tag="VN496_16625"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HXF18634.1"
                     /translation="MRWTIVFAFASAWVVSAAIADTGTQLQSTCKKPDIGYAYCLGYI
                     TGATQALSTWSAATKRDTANKTSPDAPSLYCLPRAVSNVETMQVVVKYMDEHPERLNL
                     SADTLVGYALEAAYPCAKQ"
     gene            complement(2810..3031)
                     /locus_tag="VN496_16630"
     CDS             complement(2810..3031)
                     /locus_tag="VN496_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18635.1"
                     /translation="MKTDIIRDQRRKWIWQQAREFALTGAFENFDKVVQALQVLGLNE
                     GLDPKDVLKYERSTINKLCARSRRGAYSR"
     gene            3162..3605
                     /locus_tag="VN496_16635"
     CDS             3162..3605
                     /locus_tag="VN496_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18636.1"
                     /translation="MKLRTWPAFLVAIMVTLASAPLLAQQPDSPDRGDKINSPGAHIP
                     RQQAPPPEGQQQNAEQNADQVATNQPTDNMPGHRPPDNRWHAPIAGLAGLLGITAVLA
                     HFGLVGPVASAVGGVVIIALLILIIFLIWWHMVRKYGISGRAPRR"
     gene            complement(3961..5214)
                     /locus_tag="VN496_16640"
     CDS             complement(3961..5214)
                     /locus_tag="VN496_16640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXF18637.1"
                     /translation="MAVTGIGIISPLGNEVGEFFDNLVAGRSGIERLTSAASSRRGGP
                     WSGPSVKHIGGVVKFDPAHYFAAPRLRMLDRVSQFALAAADQAVRDARITWEHVERQR
                     AGVFLGTGMGGSETTDEGYYTLYAEHSERTKPYSVLMAMTNAAASWIAIDYGLTGPNL
                     TFSTACSSSAVAIGEAARRIASGEAQIMLTGGSEAPLSFGTLVAWDALKTLACEDAAG
                     PPASCKPFAKDRSGLVLAEGAAILVLEEWQHARDRGAAIHAEMGGYGLYTDAGHITRP
                     SMEGQASAMQRALSDAQLQPEDIGYINAHGTATVANDAVETAAIRQVFGEHAYRLPVS
                     STKSMHGHLLGAAGALEFAAAVLALERNILPPTINLRVPDPECDLDYVVGQARRGVEP
                     RAVMSNSFAFGGTNAVLIARSAERV"
     gene            complement(5220..>5410)
                     /locus_tag="VN496_16645"
     CDS             complement(5220..>5410)
                     /locus_tag="VN496_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXF18638.1"
                     /translation="LDIDSLAVVEVLFDVENQFKVTVPSESGARQASLKTIGDLVEYI
                     DRLISEQHPAASEIKAAN"
BASE COUNT         1073 a         1603 c         1566 g         1168 t
ORIGIN      
        1 tctggatgtc ggcggggctc caatgccaga agcaatgggt gttgaggcca ttctgcgcgg
       61 tgcccgcgag atgtacacgg acgacgacca gttgctcagt gaagtcgaaa aaattttcaa
      121 ctatctctat ggcacattca cagcccagcc ctgaggcagc accggccggg gtttcattcc
      181 gcgacgcgtt ggtttactgg ctgaagctgg gctttatcag ttttggagga ccagccgggc
      241 agatcgcagt aatgcatcag gaactggttg aacgccgccg ctggatctca gagaggcgct
      301 tcctgcatgc gctgaacttt tgcatggtgc tgccgggtcc ggaagcgcaa cagcttgcga
      361 cctacctcgg ctggttgatg catgggacct tgggcggcat cgtcgccgga ggcctgttcg
      421 tgctgccttc gctgctcatt ctggtggcgc tatcgtggat ctacatggtc tgggaaagca
      481 ctccgttgtt cgccggcatc ttgtatggaa tcaaaccggc ggtaacagcc attgtgctgt
      541 tcgcggcgtg gcgcatcggc tcgcgcgcgc tcagaaatgc agctctatgg ggcatcagtg
      601 ccgctgcatt cgttgcaatc tattttttcc aggtgccgtt tccggccatc gtgcttgcag
      661 ccggaatgat cggtctggtg ggcggccgtc tctggccaca gaaatttgcg gcaggtggcg
      721 gccacgccga taccggaaaa acttacggcc gggcgctgat cgataacgac acgcccacgc
      781 cggctcatgc gcgcttccgg tggaacaggc tcgcgcgcgt tgctgcaaca ggcgccttgt
      841 tatggctgtt gtctttgggc gccatggctt ttgcgcgcgg gtgggatgac acgctgagcc
      901 aaatgggatg gttttttacc aaggccgctt tactgacgtt cggcggtgcg tatgcggtgc
      961 tgccttacgt ggtccagggt gccgtggagc aatatcagtg gttgactcct acacagatga
     1021 tcgatggcct cgccctgggc gagaccacac ccggcccgct catcatggtg gtggctttcg
     1081 tgggtttcgt gggtgcgtgg gcgcacgccg tcttcgggcc agattcggtg gtgctcgcag
     1141 gaatcgccgg cgccgccgtg gccacctggt ttacattcct gccctcattc gtgttcattt
     1201 tcgtcggcgc gccattcatc gaaaccacgc atcgccagcc gcatctgact gcgccgctga
     1261 ccggcattac cgccgcggtg gtcggagtca tcggcaacct tgcggttttt tttgcattgc
     1321 acgtgttctg gccacaaggc ttgcatggtg gggttgaatg gccggcaata ctgatcgcaa
     1381 ccgccgcaac tgtcgcgctc ttccgttata aggcgggcat cattccggtg atctgcgcgt
     1441 gtggtgcagc tgggctcgcg gtgcaggccg tccgagcgct tgcccagtga ttccgatcta
     1501 ccaatgaagc ggattagttg cggcgccgat cacgatttgt tttccccgct ccgccatttc
     1561 gcatattcac tacgaactat tcgtaactgg aatggcgcaa tgcttccgaa ttcgggcgtt
     1621 cgcggcgcgg cgaatagaag cgcaacttga ggcgcgtggc ccccggcgtg ccgaacgcga
     1681 tgaagcggcc gaacagatga atataacccg catatccggc agtatgagcg ccgatgcggc
     1741 gctcggagcg ccaggaggcg ccgttcccca aatcgatcaa cgaaatcgcg tgcgtacatc
     1801 caattcggcg ataccagtta tccgcgtgcg tggacaaggc ggccgcgata cgctgccccc
     1861 gatactgtaa tcgtacgaat tgtttatagc cgtagcgcgc attcccgtcg aacgcgaccc
     1921 ataccccatc gatatgcgga gtgggcccgt gtgcgaacca ctcatatccg acaaggcggg
     1981 tgccgtcaaa tgcaacagca caccgattac cgcgtgcgaa tgccgcgcgc acatggctga
     2041 ttggaagttg gattgcagcg tcactgcaat atggcagcag ttctgcctca tcaacagtac
     2101 gtaaacgcat ggccgcggtc acgtgacgct gttcaatcaa aggccgcgtg gtgatgcgaa
     2161 acagatgaag ttttagaaag cggcgaagca gactgaacag cattgacggc tctcgatcga
     2221 aatcgatttc attatgcgga caacgatcga gcatggccgt acgcttttcg gactattgca
     2281 tcgatatcgc aaagaacacc accactctca gccccctgga ctcctggcgc gcggcggtat
     2341 gcagaaccta aggccataga atccgtcaca ggaattgacc gccttgtttt gcggcgaatc
     2401 cactggaggg ttatatgcgc tggactatcg tgtttgcttt cgcgagtgcc tgggtcgttt
     2461 ctgcggcaat cgcggacacg ggcacgcaat tgcagagtac gtgcaaaaaa cccgatattg
     2521 gttatgccta ctgcctgggc tacattaccg gggccacgca agcccttagc acatggagcg
     2581 ccgcaaccaa gcgggacact gcaaataaaa cgtctcccga tgccccgagc ctgtactgtc
     2641 ttccgcgtgc agtctcgaac gtggaaacca tgcaggtcgt ggtcaaatac atggacgagc
     2701 acccggaaag gctgaacctg tccgcggata cattggtcgg ctacgctctg gaagccgcgt
     2761 atccctgcgc caaacagtag acgctgaacc aatcagcgtg ccggtccgtt caacgcgagt
     2821 acgcgccgcg gcggcttctt gcgcaaagct tgttgatggt gctgcgctcg tatttcagta
     2881 catcctttgg gtcgaggcct tcgttgagtc cgagaacctg taaggcttgc acaaccttgt
     2941 caaaattttc aaaagctccg gtaagggcga actcccttgc ctgctgccat atccattttc
     3001 gtctctggtc cctaatgatg tctgttttca tgcccttggt caggtctggt taatgaacac
     3061 gctccactgc ggatcgcttt gtggattcgg cgagacggcg agtgtgcaag caatgtgccc
     3121 ggttgcattt gcttgcatga tttttgcacc ccataggagt catgaagctt cgaacgtggc
     3181 cagccttttt agtcgccatc atggtgacgc ttgcgagcgc tccgcttctc gctcaacaac
     3241 cggactcgcc cgatcgcggc gacaaaatca attcgcccgg tgcgcatatc ccgcgccagc
     3301 aggcgccgcc gccggaagga cagcaacaaa atgctgaaca aaatgctgac caggtggcga
     3361 caaaccagcc cacggacaat atgccgggcc accggccgcc ggataaccga tggcacgcac
     3421 cgattgcagg actcgccggc ctgcttggca ttacagcggt gctcgcgcat ttcggattag
     3481 tcggaccggt tgcctcggcg gtcgggggag tcgtgatcat tgccttgctg atcctgatta
     3541 ttttcctgat ctggtggcac atggttcgca aatacggaat ttctggccgc gcaccccggc
     3601 gctgataaga gttcaccaca tagccaggta tacgttcgcg ttagcgtgga acgagcgcta
     3661 gctctctcat gcataaaaca agttcaaggg tcaccacgaa taccctggcg ttagcccgga
     3721 gtaccctttt agccacgaat accctcgcgt aagcgcggag ttatggcgaa agccggtatg
     3781 ccgaggcggc aaccctttta cacacgaaga aatacgaagg aacagggcaa tatcgatcag
     3841 tggccgcagt attgaaagaa tcactctgaa gaggcgaagc tcaccacggt accctcgcgt
     3901 tagcttcatc acggttcact tacggctgaa gctgttgtgg tgcaaatccg tcctttttcc
     3961 ttagactcgc tccgcgctgc gcgcaatcag cacagcattc gtccctccga acgcaaaaga
     4021 gttcgacatg acggcgcgcg gttcgacccc tctgcgcgct tgtcccacga cataatccag
     4081 atcgcactcc ggatccggga cacgcaagtt tatggtcggc gggagaatgt tccgctccag
     4141 ggccaacacg gccgcggcga attccagggc gcccgccgca ccgagcaggt gaccgtgcat
     4201 agattttgtc gaactgaccg gcaggcgata agcgtgctcg ccgaatacct gacgaatcgc
     4261 cgctgtctcg actgcgtcgt tcgccaccgt agcagtgcca tgagcattga tgtagccgat
     4321 gtcttcgggc tgcagctgcg catctgacag cgcacgctgc atggccgagg cctggccttc
     4381 catgctgggc cgcgtaatgt gaccggcgtc ggtgtaaagt ccatagccgc ccatttcggc
     4441 atggatggcg gcaccccggt cacgggcgtg ttgccattcc tccaatacca gtattgccgc
     4501 gccttccgcc aacacgagcc cgctgcgatc tttcgcaaac ggtttgcacg atgccggtgg
     4561 cccagcggcg tcctcgcatg ccagggtttt cagcgcgtcc cacgcaacca aagtcccaaa
     4621 cgaaagcggc gcttcgctgc ctcccgtcaa catgatctgt gcttctccgc ttgcgatgcg
     4681 ccgggccgct tctccgatgg cgaccgctga tgacgaacag gccgtggaaa aggtcagatt
     4741 cggaccggtc aatccgtagt cgattgcgat ccacgacgcc gcggcattgg tcatcgccat
     4801 caatacgcta tagggctttg tgcgttcgga gtgctcggca taaagcgtgt agtagccctc
     4861 atcggtggtc tccgaaccgc ccatgcctgt gccgagaaag actccggcgc gttggcgctc
     4921 cacatgctcc cacgtaatgc gcgcgtctcg caccgcctga tccgcagcag cgagtgcaaa
     4981 ttggctgacg cgatcgagca tgcgcagtcg tggcgctgca aaatagtggg caggatcaaa
     5041 tttgaccacg ccgccaatat gcttgacgga tggaccgctc catggcccgc cacggcggct
     5101 ggacgcggcg ctcgtcagcc gctcgatccc cgaacggccc gcaacgagat tgtcgaaaaa
     5161 ctcgcccact tcattaccca gtggcgagat gattccaatc ccggttactg ccacacgttt
     5221 caattcgcgg ccttaatttc gctggcggcg ggatgctgtt ccgagatcag gcggtcgata
     5281 tactcgacca gatcgccgat cgtcttgagg ctcgcttgac gggctccgga ctccgacggg
     5341 acggtgacct tgaactggtt ctccacgtcg aacaacactt caacgactgc cagcgaatcg
     5401 atatccagtt
//
LOCUS       DATMZQ010000521         4728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2335213, whole genome shotgun sequence.
ACCESSION   DATMZQ010000521 DATMZQ010000000
VERSION     DATMZQ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335213"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VN496_16650"
     CDS             <1..589
                     /locus_tag="VN496_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXF18639.1"
                     /translation="APIVRVPAGELAMGARCLDGGALGVVIPHVDTPEQARAIADAFR
                     FPPLGHRSIGGAYPHFAFMSRPVNEVVTGLDGATLVIAMIETPRAVENADQIAAVPGL
                     DVLLIGTNDLCLEMGIPGQLDHARVRAAFDSVLSACKKHRKIPALGGIYGKELLARYL
                     NLGFRMVLAGNDISLLHAAAQEQAKFVRGLGSGPR"
     gene            664..1185
                     /locus_tag="VN496_16655"
     CDS             664..1185
                     /locus_tag="VN496_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18640.1"
                     /translation="MKLGGFWIGWAFCGYLSVVVAGAALLHYLRRMPRFFSVLSLPGT
                     LAHEMLHFLLGTLTLAQPVKFSLLPKFHRDGSATLGYVTFNNIRWYNALWVGMAPMLA
                     LPVSLWLVYYRSMQSAPSAIQEAAWSYVSASLLYSCLPSKADIAIVWSKPVGLIVYLA
                     SGVMLYWMRFTSS"
     gene            1350..1625
                     /locus_tag="VN496_16660"
     CDS             1350..1625
                     /locus_tag="VN496_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18641.1"
                     /translation="MMSKLLTITAAAALVLSLGGLARAADQNDQNTPQPQTRPQVQQP
                     VDDPAAGGATATQAYQEYLVSLKKCEPLNGEEREKCVDAARRQHGQM"
     gene            1777..2988
                     /locus_tag="VN496_16665"
     CDS             1777..2988
                     /locus_tag="VN496_16665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXF18642.1"
                     /translation="MREAVLKYQGYSVAAMRDLARKRLPRSVFDFVDGGAEDEVALQR
                     NEAAFREIAFLPNPLKGTTRRDQSIDLFGARLPSPVLIGPTGLTGMLWPRGEIAAARA
                     AAAVGTVYTMSHGSTVKIEDLAAQVKGRQWMQIFAYKDRGLTENFVHRAHDAGYQALV
                     LTIDNQVLGQRERDLRNGFTVPPRFEIGSLVDMAFHLRWLLRMARAPRLAMANYEREG
                     DTGVFSIGARVVGWMDPSSSWKEVEWLRRIWDRPLLLKGVLHPDEARRARALGVDGII
                     VSNHGGRQLDVVPASLHALPAVVDAVSGKMPVLFDGGVRRGGDVLKAIALGATACLIG
                     RPHLWGVAVAGEQGVARVLEIYRSEIERIMALCGWDCLKEIDHSVLFKPGGKSESVLS
                     TFSKKLTVMLG"
     gene            complement(3027..3905)
                     /locus_tag="VN496_16670"
     CDS             complement(3027..3905)
                     /locus_tag="VN496_16670"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXF18643.1"
                     /translation="MQTQAGQQESTWPFRLRPLSQNLGAEIIDVDLTHGVVDEQFAAI
                     YQAFVRYQVLLFGTQQLPPGRQVEFARRFGPVQVHVMNQYHADGYPELYRLSNLDADG
                     NPNGQHPDKGTLAWHTDGSWQRITGQATIIYAEVVAGEGGETHFCDMYGAYERLTPAW
                     KQRIQNLRAVHNLDFSRTRRHAEQRMTEAQRSSVPPVDHPIVRTHPDTGRKCLFLGDH
                     AEYVLGMDYEQGRALIEELNALAIHADLTYQHRWTPHELIVWDNRSVLHRATSYDPAT
                     QKRVIRRCTVLGEAPR"
     gene            complement(3993..>4728)
                     /locus_tag="VN496_16675"
     CDS             complement(3993..>4728)
                     /locus_tag="VN496_16675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXF18644.1"
                     /translation="AIDYDARDLSPAEEIERLSKVPLAHVPGTVWEYSLASDVLGRVV
                     EAASGERLADFLDKRLFKPLKMNDTGFWVAQDKINRLAKALPTDINSGKPNNLIDVTV
                     TPANDSGGAGGVSTAGDYLRFSQMLLNGGQLEGTRVLSRNTVTLMTSDHLGTRISAPI
                     TPGELLLGVPGYTFGLGFAVRQGPGIAGVPGSAGEFTWAGYAGTYFWVDPKEEIAAVM
                     MTQAPSPIRAYYRRLFRQLVYAAIAD"
BASE COUNT          917 a         1341 c         1447 g         1023 t
ORIGIN      
        1 cgcaccgatc gtgcgcgttc cggccggcga acttgccatg ggtgcgcgct gcctcgatgg
       61 cggggcactg ggcgtggtca ttccgcatgt cgatacgccg gaacaagcgc gcgccatcgc
      121 ggatgcattt cgtttcccgc cactgggtca tcgctccatc ggcggtgctt atcctcattt
      181 cgctttcatg tcgcgtccgg tgaacgaagt cgtcactggc ctggacggcg ctacccttgt
      241 gatcgcgatg atcgaaacac cgcgtgccgt agaaaacgcc gatcagatcg cggcagtccc
      301 tggtttggat gtcctgttga tcggcaccaa cgacttatgc ctggaaatgg ggattccggg
      361 ccagcttgac catgcccgcg tgcgagcggc gttcgacagc gtgctgagtg cttgcaaaaa
      421 gcaccgcaag attccggcac tcggtggcat ctatggcaaa gaattactgg cgcggtatct
      481 gaatttgggc tttcgcatgg tattggcggg aaacgacatt agcctgttgc acgccgccgc
      541 tcaagaacag gccaaatttg tgcgcggatt gggctcggga ccgcgctgat cgcatgcttc
      601 tcgtgatcgc tctagttcgc gacgtcgatg cgacgaacgc gtaaatccga ctcgaaccac
      661 tgaatgaaac tcggtggttt ctggatcggg tgggcgttct gcggctatct gagcgttgtc
      721 gttgcaggcg ccgctctatt gcattatcta cgccggatgc cgcgcttttt cagtgtgctt
      781 tcgctgccgg gcacgctggc tcacgaaatg ctgcattttc tgttgggtac actgaccctg
      841 gcgcagccgg tcaagttttc tctgctgcca aagtttcatc gcgacggttc ggcgacgctc
      901 ggttatgtga ccttcaacaa catccgttgg tataacgccc tttgggtcgg catggcgccg
      961 atgcttgctt tgcctgtgtc gctttggctg gtgtattacc gttccatgca gagcgcccct
     1021 tctgccattc aggaagcggc gtggagttat gtttcggcaa gtctcctgta ctcttgcctg
     1081 ccgtcgaagg cggatattgc tatcgtttgg tccaaaccgg ttgggctgat tgtttatctt
     1141 gcaagcggcg tgatgctgta ctggatgcgg ttcacctcca gttgaagcct cagtcgcacg
     1201 gtgcatgttc cactgcgagt ctccaccgga cgacattttc cttgaaccag aatgcgtgcg
     1261 atcggactaa atactgtcgg tcactacgat cgtcgtccgc accgcggacg gaaattcgtg
     1321 ggtgacatgt caagacgagg agaaagatca tgatgagcaa gctactgacg ataacggctg
     1381 cagcggccct ggtcctgtcg ctgggaggat tggccagggc agctgaccaa aacgatcaga
     1441 atacgcccca accgcaaact cgcccgcagg tccagcaacc tgtcgacgac ccggccgccg
     1501 gcggtgctac ggcgacgcag gcttaccagg agtatctggt gtcgctcaag aaatgcgagc
     1561 cgcttaacgg ggaagagcgg gaaaaatgtg tcgatgcggc acgcaggcag cacggtcaga
     1621 tgtgattgct gaaacaaatc agtgggaaga cggcaccatt ggtgccgttt tttttgcata
     1681 gtccgtttgg ctggagaccg agctccaggt aattttttgc ttttccgcga cggttgaacg
     1741 acgattgaac tctggccgag tcggacggag gagtgcatgc gggaagcagt tttgaaatat
     1801 cagggctaca gcgttgcggc catgcgcgat ctggcgcgca agcgcttgcc acgctcggta
     1861 ttcgatttcg tcgatggagg agcggaggac gaagtcgcat tgcagcgcaa tgaagcggca
     1921 tttcgcgaga tcgcattctt gcccaacccg ctcaaaggga caacgcgccg ggatcagtcc
     1981 attgatttat tcggcgcgcg tttgccgagt ccggttttga ttggaccaac cggcctcacc
     2041 ggaatgctat ggccgcgtgg tgaaatcgca gcggccaggg cggctgctgc agtgggcacg
     2101 gtctacacca tgagccatgg ctcgacagtg aagatcgagg atctcgcagc gcaggtgaag
     2161 ggcaggcaat ggatgcagat attcgcttac aaggaccgcg gactgacgga aaattttgtg
     2221 cacagggcac acgatgcggg ctatcaggcg ctggtactga ccatcgacaa ccaggtactg
     2281 ggtcagcgcg agcgcgatct gcgcaacggc ttcaccgtgc cgccccgatt cgaaatcggc
     2341 agtctggtgg acatggcatt tcatttgcgc tggcttctgc gcatggcgcg cgcgcccaga
     2401 cttgcaatgg ccaactatga acgcgaggga gataccggcg tcttttcgat cggggcccgc
     2461 gtggtggggt ggatggatcc gagttccagc tggaaagaag tggagtggct gcgccgcatc
     2521 tgggaccggc cgctattgct caaaggcgtc ctgcatcccg atgaagcgcg ccgggcgcgg
     2581 gccttgggcg ttgacggcat catcgtatcg aaccatggcg gccgccagct cgatgtcgtg
     2641 cccgcatcgc tgcatgcgct gcccgctgtc gtggatgcgg tatccggaaa aatgccggtc
     2701 ctgttcgatg gcggggtgcg gcgcggcggt gatgtactga aggcaatcgc gctcggtgcg
     2761 acggcctgcc tgattggcag gccgcatctc tggggggtag cagtggccgg ggaacagggc
     2821 gtggcgcggg tactggaaat ctaccgcagc gaaatagaac gcatcatggc cctgtgcggc
     2881 tgggactgcc tcaaggaaat cgatcattcg gtgctgttca aaccgggtgg caaatcagaa
     2941 tccgtgctgt ccacgttcag caaaaagctg acagtgatgc tgggatgaaa cgcgttaccg
     3001 cctgaaatta tttatgttgg tttgaattat ctcggcgcct caccaagcac ggtgcagcgt
     3061 ctgataacgc gcttctgcgt tgccgggtcg taagaagtcg ctcggtgcaa cacgctgcga
     3121 ttgtcccaga cgatcagttc gtgcggcgtc cagcgatgct ggtaggtcag atccgcgtga
     3181 atcgccagcg cgttcagttc ctcgattagc gcacggccct gctcgtaatc cattcccagt
     3241 acgtattccg catgatcgcc caggaacagg cacttgcggc ccgtgtccgg gtgcgtgcgg
     3301 acgatcgggt gatcgaccgg tggaacggaa ctgcgctgcg cttccgtcat gcgctgttcg
     3361 gcatggcggc gcgtgcgcga aaaatccagg ttgtgcacgg cgcgcagatt ctggatgcgc
     3421 tgtttccacg cgggcgttag acgctcatat gcgccgtaca tatcgcaaaa atgggtttca
     3481 ccgccttcgc ctgcgacaac ttccgcataa atgatcgtgg cctgaccggt gatgcgttgc
     3541 cacgaaccat ccgtatgcca ggctagcgtg cccttgtcgg gatgctgccc gttcggattg
     3601 ccgtcggcat ccagattgga aagccggtaa agctcggggt aaccatccgc gtgatactga
     3661 ttcatcacat ggacttgcac cgggccaaag cggcgggcaa attcgacctg gcgccctggc
     3721 ggtaattgtt gcgtgccgaa cagcagcacc tggtagcgaa cgaaagcctg ataaatcgcc
     3781 gcgaactgtt cgtcaaccac gccatgggtc aaatcgacat cgatgatttc cgcaccgaga
     3841 ttctgggaga gcggccggag ccggaacggc cacgttgatt cctgttgtcc ggcttgcgtt
     3901 tgcataaggg aagagtttcg ctccagtcag tcgtcacgtc gtcacgtcgt cacgtcgtca
     3961 cgtcgtcacg tcgtcacgtc gtcacggccg cctcaatccg cgatcgccgc atacacgagt
     4021 tgcctgaaca gccgtcgata ataggcgcgg atcgggctcg gtgcctgcgt catcatgacg
     4081 gcagcaattt cttctttggg atcgacccag aaataagttc cggcataacc ggcccacgtg
     4141 aattcgcccg ctgagcccgg cacgccggca atgcccggtc cctggcgcac cgcgaagccc
     4201 aatccgaacg tgtaaccggg aacacccagc agcagttcgc ccggcgtgat cggcgcggag
     4261 atccgtgtgc cgagatgatc cgaggtcatt agcgtgacgg tattgcggct caatacacga
     4321 gtgccttcca gttggccgcc attgagcagc atctgcgaaa aacgcaaata atcccccgcg
     4381 gtcgacacgc cacccgcccc gccggaatca ttagccggtg tcaccgttac atcgatgagg
     4441 ttgttaggct tgccgctgtt gatgtccgtg ggcagcgctt tggccagccg gttaattttg
     4501 tcttgtgcga cccagaaacc ggtgtcgttc atcttcagcg gcttgaatag ccgcttgtcg
     4561 agaaaatcgg ccagccgttc tcccgatgca gcttccacga cgcgtcccag cacatcgctc
     4621 gccagactat attcccaaac cgtgcccggc acgtgcgcca gaggcacttt cgaaaggcgc
     4681 tcgatttctt ccgcaggcga taaatcgcgt gcgtcataat caatcgcg
//
LOCUS       DATMZQ010000522         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2336265, whole genome shotgun sequence.
ACCESSION   DATMZQ010000522 DATMZQ010000000
VERSION     DATMZQ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336265"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..569
                     /locus_tag="VN496_16680"
     CDS             213..569
                     /locus_tag="VN496_16680"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXF18645.1"
                     /translation="MGYCHCDSCRRWSAGPVNAFTLWKPDALRITKGAQNIGSYHQTP
                     RSHRKWCKNCGGHLFTEHPLWGLTDVYAAVIPGFEFKPAVHVNYQETVLPIRDGVPKL
                     KDVPKEMNGSGISIAE"
     gene            complement(608..1591)
                     /locus_tag="VN496_16685"
     CDS             complement(608..1591)
                     /locus_tag="VN496_16685"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXF18646.1"
                     /translation="MDALSEVLRAVRLTGAVFFDLQLRAQGSYLTAPARKIADVIMPD
                     ADHVIPFHLITQGTCYARLPDGDFSKLGAGDLILFPAGDRHVLATPGALQAKPTDITG
                     ESLDELKLGHIAEFNSGSAGPTTRIICGFLACDKRLAEPILLGLPRLLKVSVGDGGTG
                     AWIQSSIRYSVAESAASRPGSAMVLSRLSEVLFAEAVRHYIDQLPPGQSGWLAALRDR
                     HVGRTLSLLHERPAHPWTVEELAATIGLSRSALGQRFQALIGVAPMQYLTRWRISLAA
                     AQLRESNVSIVQVATDVGYESEAAFNRAFKRELGLPPATWRKRAVVTSSAS"
     gene            1815..2570
                     /locus_tag="VN496_16690"
     CDS             1815..2570
                     /locus_tag="VN496_16690"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18647.1"
                     /translation="MRFLLLPLLVVFLAAAAVPAIGADSASLANTVVSASDRSPEDRA
                     MDAGRKPAELLTFLGIQPGWRVADLGAGGGYTTELLQRAVGSNGQVYGQNSKRLLEMF
                     LEKPWSARLAKPVMKSVVRLDRDFDDPFPADVTALDAVTMVLFYHDTVWLGVDRPAMN
                     KAVFDALKPGGVFLIVDHSARPGDGTDVASTFHRIEEDVVKQELQAAGFVLDGEANFL
                     RNSQDTRDWNDSPRAAGDRRGTSDRFVLRFRKP"
     gene            2582..2887
                     /locus_tag="VN496_16695"
     CDS             2582..2887
                     /locus_tag="VN496_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HXF18648.1"
                     /translation="MALELRPNCECCDRDLPPASTAARICTYECTFCADCVESVLFNV
                     CPNCGGGFERRPIRPATEWRPGLSIAKRPPSDKRRSLNYSREDIAAHSARIRNIAPE"
BASE COUNT          644 a          842 c          871 g          574 t
ORIGIN      
        1 acaacctgcg ataccggacg caagccgtaa acctttggcg aggaccaaag taatgtcctg
       61 cgacaaccca aatctttatt cgcttcaata ctaatcattg cccgcgacag tttcgggcag
      121 ctaacaaggg acttatggaa aagcaaagca gctatgacgg atcttgtttt tgcggcgcag
      181 tcgcattcac cgttagcggt gggccggtcg caatgggata ttgtcattgc gactcgtgcc
      241 gtcgctggtc cgccggtccg gtgaatgcat ttacgctttg gaaaccggat gccttgcgca
      301 ttaccaaagg cgcgcagaac atcgggtcct atcaccagac accacgcagt catcgcaaat
      361 ggtgtaaaaa ctgtggcgga cacctcttta ccgagcatcc tctatggggg cttaccgatg
      421 tgtacgcggc cgtcataccc ggctttgagt tcaagcctgc cgtgcatgtc aactatcagg
      481 agaccgtatt gccgatcagg gatggcgtgc ctaaactaaa agatgttcca aaagaaatga
      541 acggttccgg gatcagcatt gcagaatgag tgatcggccc agttcacatt gcaaacaatg
      601 gagaagccta gctagcggaa ctcgttacga ctgcgcgctt gcgccaggta gcgggaggca
      661 atccaagctc gcgcttgaac gcccggttga aggccgcttc ggattcgtag ccgacatcag
      721 tggcgacctg cacgatggaa acattgcttt cgcgcaactg tgcagcagca agtgaaatgc
      781 gccagcgtgt cagatattgc atcggcgcga cgccaatcag cgcctggaat cgctgaccca
      841 gcgcggagcg cgacagcccg atcgtcgctg caagctcctc gacggtccac ggatgcgccg
      901 gccgctcgtg aagcaacgat agtgtccggc cgacgtgccg gtcgcgcagg gccgcaagcc
      961 aacctgattg accgggcggc aattggtcaa tgtaatgccg gacagcttct gcaaacagta
     1021 cctcggacag tcgcgacagc accatggccg agcctggacg cgaagcggca ctttccgcaa
     1081 ccgagtaacg gattgaactt tgaatccatg cgccggtacc gccgtcaccg acactgactt
     1141 tcagcagtcg cggaagaccc agcaggatcg gttcggcaag acgtttgtcg caggccagaa
     1201 atccgcagat gatgcgcgtc gttgggccgg cactcccgct attgaattcc gcgatatgtc
     1261 cgagcttgag ctcatccagg ctttcgccgg taatgtcggt gggtttggcc tgcagtgcac
     1321 cgggcgtcgc gagcacatgc cggtcgccgg ccggaaacag aatcaggtcc cctgcgccaa
     1381 gtttgctgaa atccccatcc ggcagccgtg catagcaggt gccttgcgta atcaaatgaa
     1441 acggtatgac atgatccgcg tccggcatga tcacatccgc gattttccgg gccggcgcag
     1501 tcaaatacga cccttgcgcg cgcaattgca gatcgaaaaa taccgcgcca gtaagccgaa
     1561 ccgcacgcaa aacctccgac agtgcgtcca tggcgagtca ttctcccgag cgaagacacg
     1621 cattaaacat ggcaatgcag gcggcatcgg tcatcgaaaa taagaggggc cggtagtgaa
     1681 gcagcacgac ggggggatgc atactacgac ggaagatcga ttttggcaaa gatgggttct
     1741 gggtacaatt ggcacattgt ctgccatcgt cggaaaccag atccatccgg atccaaacca
     1801 atcgggagtg ccaaatgaga ttcttgctgc tgcctttact tgtcgtgttt ctcgctgcgg
     1861 ccgcggtgcc tgccatcggc gcggattccg cgtcgctcgc gaacaccgtt gtctccgcat
     1921 ccgaccgcag tcctgaagat cgggccatgg atgctggccg caagcccgcc gaactgctga
     1981 ccttcctcgg catacagccg ggttggcgcg ttgccgacct gggcgctggc ggaggctata
     2041 cgaccgaatt actgcagcgc gcagtgggca gcaacggcca ggtgtacgga cagaattcca
     2101 agcggctgct ggaaatgttc ctggaaaagc cctggagcgc gcgcctggca aaacccgtaa
     2161 tgaagagcgt ggtgcgcctc gatcgggatt tcgacgatcc gttccctgcc gatgtgaccg
     2221 cgctcgatgc ggtgacgatg gtgctttttt atcacgatac ggtttggctt ggcgtggacc
     2281 ggcctgcaat gaacaaggcg gtattcgacg cgctgaagcc cggcggcgta ttcctgattg
     2341 ttgatcacag tgcgcggccg ggagacggta ccgatgtggc cagcacgttt caccgtattg
     2401 aagaagacgt cgtgaagcag gagctccaag cagcggggtt cgtgctggac ggtgaagcca
     2461 atttcctgcg caatagccag gacacgcgcg actggaatga ttcgccgcgt gccgccggag
     2521 accggcgtgg caccagtgat cgcttcgtgc tgcgctttcg caagccctga agaggaacag
     2581 aatggctctt gaactgagac cgaactgcga atgctgcgac cgcgaccttc cgcccgcatc
     2641 cacggctgca cgcatctgca cttacgaatg tacgttttgt gccgattgcg ttgaaagcgt
     2701 gctgttcaat gtgtgcccga actgcggcgg cggcttcgag cgccgcccca ttcggcccgc
     2761 gacggaatgg cggccaggtc tttcgattgc caagcggccg ccctcggaca agcgccggtc
     2821 actgaattac agcagagagg atattgccgc gcacagtgca cggatcagga atatcgcacc
     2881 ggaatagcgc tgaacatttg gctttaaggt gcgcgcccgg ttcggacgaa a
//
LOCUS       DATMZQ010000523         8500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2337228, whole genome shotgun sequence.
ACCESSION   DATMZQ010000523 DATMZQ010000000
VERSION     DATMZQ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337228"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..970
                     /locus_tag="VN496_16700"
     CDS             197..970
                     /locus_tag="VN496_16700"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXF18649.1"
                     /translation="MPTLAWIIAATLTSGVLSVIAAASLALRAKPAQLPMLISYAIGA
                     LLGAAFLEILPHAVELADSVKVVTGTVLIGILLFFVLEKMVLWRHAHEGQAESHVPEH
                     DHGRIATMVIVGDTFHNCVDGVIIAGAFLVSLEVGIVTALAIVAHEVPQEVSDFLILL
                     HSGYSRRLAFGFNILASSAMVVGGVFAYYALQLMHNAIPPLLGIAAASMIYVSVADLI
                     PGLHRRRDFRATLQQVTLIAAGIGTVWIVGYLAGAVANV"
     gene            998..1471
                     /locus_tag="VN496_16705"
     CDS             998..1471
                     /locus_tag="VN496_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18650.1"
                     /translation="MFMTAWCLITLVIAALLLGSSFARVLEIGPMRRYGGPQWLELQH
                     SLYPSYAAAAGIIEPAAIIAAAMLAVVLRDWRPALLVAIAGAVCLAIAFAFVSLPVTN
                     RINARISRWTADALPPNWEIWRTRLEVSQVLRFILHLAGFALLVVATLVSPLSDE"
     gene            complement(1510..2739)
                     /locus_tag="VN496_16710"
     CDS             complement(1510..2739)
                     /locus_tag="VN496_16710"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXF18651.1"
                     /translation="MSAAPRAQRRVVIIGGSLVGLITGNLFHRAGWDVQVFERVSGNV
                     EGRGAGITILPGLVDGFCAAGAQVSEQSLGVELATRIALDKSGRIVAERDFSQVMTSW
                     KRLYDELKNVFPSENYRAGMALQTVEQDDRHVIACFNEGERIEADLLVAADGLRSTVR
                     AQFLPEIKPHYAGYIAWRCLTDEGDLSAAAHAQLFKRYSVCVAPGQQGVGYPVPGPDN
                     SVEPGRRQYNTVWYCPVPEAGLRVLMTDDSGHYHANGIPPTLLTARIREQMIDKAAQV
                     LAPQFAEAVRCARLHFFQPIVDLEVPRLVFGRVVIVGDAAFTARPHVAMGVPKGAGDA
                     LALMKAVGTDSDPLAALAHFEAERLRIDAAIVARGRYLGAYMEAQLRSEAERARADAL
                     RVPETVMMETAAPLVFS"
     gene            complement(2736..3653)
                     /locus_tag="VN496_16715"
     CDS             complement(2736..3653)
                     /locus_tag="VN496_16715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXF18652.1"
                     /translation="MASLPHIGWIGAGRMGVPMAGFILKAGYPLSIYSRSAASRAQLV
                     AQGAREALSTQECVREADIIFAALPDDAALRDVAFGPRGVLAHARSESVFVDTSTVSP
                     QISAEIARAAAERSVGYLRMPISGNAASARTGNITVLVSGPEALWRKVRPVVETFSKN
                     QAYLGGTEQARYMKLVINAVVVSTAQAMAEALTLGRTAGLEWDLMLDTIAESTIASPW
                     LRAKIALLKPRDFTPTMTPELILKDMGLMIAAATEGGTRMPLIELTREIWQGLLSGDY
                     AHEDYMAAVKLAEQHAGLNSEAMDQRKPK"
     gene            complement(3678..4406)
                     /locus_tag="VN496_16720"
     CDS             complement(3678..4406)
                     /locus_tag="VN496_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18653.1"
                     /translation="MTKLAMEGIDAGYGAVRVLHGVSLDVRDGETVALLGTNGNGKST
                     LMKCIMGLLKPTRGSIVLEIDGVSIDLVGKTPHEIVGLGVALVPEGRRLFPKLTVAEN
                     LLLGAYRSGARAAIARNLSLCHEAFPVLGQRSAQRAGELSGGQQQMLAIARALMSAPK
                     LLLVDEPSVGLSPMLVKQMIDTIASLKRQIGLTVLMAEQNFHQASRIADRGYLIVQGA
                     IAFEGRTMSELHSSELVKACYLGA"
     gene            complement(4399..5127)
                     /locus_tag="VN496_16725"
     CDS             complement(4399..5127)
                     /locus_tag="VN496_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18654.1"
                     /translation="MNGALLAVNAVSKLFGGFVALADVSLTVRSGERLGIIGPNGSGK
                     STLINCIGGSLGTDSGSIWLSGADVTLLPAHRRARLGIARTFQVPRPFASMTLAENIA
                     VPLEYVTHRKSLHRSDVAAQAHAILAQIGLGGRADASPASLTQVDLRKLELARALASR
                     PRLLILDEVMAGLSAIEVDEMLAILLALNREGIAIVMIEHIMRAIMRFSERVICLDAG
                     RILAHGAPAQIVEDRDVQRIYLGD"
     gene            complement(5124..6074)
                     /locus_tag="VN496_16730"
     CDS             complement(5124..6074)
                     /locus_tag="VN496_16730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF18655.1"
                     /translation="MPTPWKFRLAVLALLATALLLARIVSNEYFFFAGYVVLQFIVLA
                     TAWNILGGYAGYVNFGASAFFAAGAYTALALIKAFAAPLILQLAGAALVSGLMGLIVG
                     AMTMRLRGIFFSIATVAIAVICETVVLNWSFVGGARGATVLQPAAPALFSSYARLLFV
                     VMALAAVSAVLLARYIETSWIGRGLRALRDDEEAAESAGVPTLKLKLFAATTSGALMG
                     IAGAPFPLYMSFIEPTSAFSLNYAVSALAMPIIGGTASWTGPVIGAVLLSSVQQLVTV
                     TLSSELNVLVLGVLLVAFVVAAPEGILGLVRRWYSAEHTK"
     gene            complement(6079..6948)
                     /locus_tag="VN496_16735"
     CDS             complement(6079..6948)
                     /locus_tag="VN496_16735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXF18656.1"
                     /translation="MSIDLLGEAIIAGVLLGCFYAAVSVGLSVAFGLLDVPQIAHPAF
                     LVLGAYGAYYLGGFGVDPLVAGLVLTPLFFLGGLAVYRFYHASFESRGKDVGLRGLAF
                     FFGLAFIIEVALILTFGVDQRMVEAFYIGKSVSLGDMRLPLRMLVAFGVALVLTIGLT
                     FFFARTFIGRAIKAVAQDETAVALMGADPVRIKHWAFAIATATCAIAGALLIIVTPVE
                     PGLGRLYIGRTFCVAVLAGLGSMPGTLAAGVILGVTESIVLTSLGASWAPAVAFGLLL
                     LVLAIRPAGLFGR"
     gene            complement(6980..8239)
                     /locus_tag="VN496_16740"
     CDS             complement(6980..8239)
                     /locus_tag="VN496_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXF18657.1"
                     /translation="MQSPGRRIVAGIAGVIAVLLLCSQVGTYAQNKTPIRIGLSIAQT
                     GPLSGAGKSGLLALQIWRDDVNAAGGLLGRPVELVVYDDQSSPSMTPGLYSKLLDVDK
                     VDLLVAPYATNPTAPIMPLVKQRDRLLIGNFALDANAKIGHDKYFNNQPWASARDSAA
                     PFLLLCRKVGAKTIAIIAADAEFAQNLAGGVRSGLKESGLEAVYDQNYPPNTVEFSSV
                     VRAIRAKNPDVVFVASYPSDSSAIIRSVAEIGIGDNVKLFGGGMVGLQYASIMQSLGS
                     SLNGVVNYNTWAPEKTMDFPGIKDFLMRYQAKAAQANVDPLGFYLPPFDYAIGQILAQ
                     AVTATRSLDDKVLAKYIRAHEIKTIVGSIHYDPSGEWANPRIVYVQFRDIADRNLEQF
                     RAPGKQVIVAPEAFKTGELKPFNQSRP"
BASE COUNT         1740 a         2684 c         2485 g         1591 t
ORIGIN      
        1 tgacctcccc ccggtcaact cagcgcgcga catttgtcgg gacttgccgc caaagtctgc
       61 ggcgattgca gcaagtttac aagcgccagt cgatgcaccc catgtgctgc gccgcaatgc
      121 ccggggcacg cgctgtagtg cgaaacgccc gaggggtctg ctacagtgcc tgcctctttg
      181 aactcccggt agccgcatgc ccacccttgc ctggatcatc gccgccacgc tgaccagcgg
      241 agtactcagc gtcatcgccg ctgcttccct ggcattgcgc gccaagcctg cgcaactgcc
      301 gatgctgatc agctatgcaa tcggcgctct gctcggtgcg gctttcctgg aaattctgcc
      361 tcacgcggta gagctcgccg acagcgtcaa ggtggtcacc ggaaccgtgc tgatcggaat
      421 cctgcttttt ttcgtgctgg aaaaaatggt gctgtggcgg catgcccatg aggggcaagc
      481 agagtcacac gttcccgagc atgaccacgg tcgcatcgcg acgatggtga tcgtgggcga
      541 cacattccac aattgcgtgg acggcgtgat catcgccggc gcattcctgg tgagcctcga
      601 ggtcggcatt gtcactgcgc tcgcaatcgt cgcgcacgaa gtcccgcagg aagtcagcga
      661 cttcctgatc ctgctccatt ccggctacag caggcgcctc gcatttggct tcaacatact
      721 ggccagcagc gccatggtcg tgggcggcgt tttcgcctat tacgcactgc agctgatgca
      781 caatgccata ccgccgctgc tcggtatcgc ggcagccagc atgatctacg tttcggtcgc
      841 ggatctgata ccgggactgc atcgccggcg cgactttcgc gcaacgctgc agcaagtaac
      901 gctcattgcg gccggcattg gtacggtctg gatcgttggc tatctggccg gggcggtggc
      961 aaacgtctga acttccctcg ccgcgagagg atccgttatg tttatgactg catggtgcct
     1021 gatcacgctg gttatcgccg ccctcttgct cggcagcagc ttcgcacgcg tgctcgaaat
     1081 aggaccgatg cgacgctatg gcggcccgca atggctcgag ttgcagcact cactctatcc
     1141 gtcctatgca gcggcagccg ggatcataga gcccgctgca atcattgctg cagccatgct
     1201 cgccgtcgta ttgcgcgatt ggcgcccggc gctgctcgtc gcgattgcgg gtgcggtctg
     1261 cctggcaatt gcttttgcat ttgtttcgct accggtcacg aatcggatta acgcgcggat
     1321 ttcacgatgg acggcggacg cacttccgcc caattgggaa atctggcgca cgcgtctgga
     1381 agtgtcccag gtgttgcgtt tcatcctgca ccttgccggc ttcgctttgc tggtagttgc
     1441 caccctggtc agtccgctgt ccgacgaata actacggcgc agaagaatcc acgcgcggtg
     1501 ttgagggcct catgaaaata ccagcggcgc tgccgtttcc atcatcacgg tttccggcac
     1561 acgcaacgca tctgctcgcg cgcgttcggc ctctgatcta agctgcgctt ccatgtacgc
     1621 gccgagatag cggccgcgcg caacgatggc cgcgtcgatg cgcaggcgct ctgcttcgaa
     1681 gtgcgccaat gcggccagcg ggtcgctgtc cgtccccacc gctttcatta atgccagcgc
     1741 atcgccggca cccttcggaa cgcccatggc aacatgcggc cgtgcggtga aggcggcgtc
     1801 gccgacgatg acgacgcgcc cgaacaccag acgcggcacc tcgaggtcga cgatcggctg
     1861 gaagaaatgc agcctggcgc atcgcacagc ttccgcgaac tgcggcgcga gtacctgggc
     1921 agccttgtcg atcatctgct cccgaatgcg cgcggtcaac agagttggcg ggatgccgtt
     1981 tgcatgatag tggccgctgt cgtccgtcat tagtacacgc aaccctgctt caggcacggg
     2041 gcaataccac accgtgttgt attgcctgcg gccgggctcg acgctgttgt ccgggcccgg
     2101 caccggatag cctacgcctt gctgcccggg tgcgacgcat accgaatagc gcttgaacag
     2161 ttgcgcatgc gccgctgcgg aaagatcgcc ttcatcagtc aggcagcgcc aggcaatata
     2221 gcctgcgtaa tggggtttta tttcaggcag gaactgggcg cgcaccgtgg agcgcaatcc
     2281 atctgcggca acgagcagat cggcttcgat acgctcacct tcgttgaaac acgcgatcac
     2341 gtgccgatcg tcctgttcaa ccgtttgcag agccatgccg gcacggtagt tctccgatgg
     2401 aaagacgttc ttcaattcgt cgtacagccg tttccaagat gtcatgacct gcgagaaatc
     2461 acgctcggcc acaatccggc cggacttgtc gagtgcgatg cgtgtcgcaa gctcgacgcc
     2521 gagtgactgt tcggacacct gcgcaccggc cgcgcagaag ccatccacga gtcccggcaa
     2581 tatcgtgatg ccagcaccgc ggccttccac attgccgctc acgcgttcaa atacctgcac
     2641 gtcccagccc gcgcggtgaa acagattacc ggtgatcagc cccaccagcg aaccgccaat
     2701 gatcaccact cggcgctgtg cgcgcggcgc ggcgctcatt tgggtttccg ttggtccatg
     2761 gcttcgctgt tcagtcccgc gtgctgttcg gccagcttga ccgctgccat gtaatcctca
     2821 tgcgcatagt cgccactgag caggccttgc caaatttcac gcgtaagttc gatcagcggc
     2881 atgcgggttc cgccttcggt cgctgcggca atcatgaggc ccatgtcttt caggatcagt
     2941 tccggggtca tggttggcgt gaaatcgcgt ggcttgagca gagcgatctt ggcccggagc
     3001 cacggcgacg cgatggtgct ctcggcgatg gtgtcgagca tcagatccca ttcgagtccc
     3061 gcggtgcgcc ccaaggtcag tgcttcggcc atggcttgcg cggtgctgac gaccaccgcg
     3121 ttgatcacca gcttcatgta acgcgcctgt tccgtgccgc cgagatacgc ctggtttttg
     3181 ctgaacgtct caaccacagg tcttaccttg cgccataacg cctctggtcc cgacaccagc
     3241 acggtgatgt tgcccgtgcg cgccgaagct gcattgccgg aaatgggcat gcgcaaataa
     3301 ccgacggagc gctctgcagc tgcacgcgca atttctgcag atatctgggg cgatactgta
     3361 ctggtatcca cgaatactga ttccgacctg gcatgggcga gtactccgcg cgggccgaac
     3421 gccacgtctc gtaacgccgc gtcatccggg agggcggcaa aaataatgtc cgcctcacgc
     3481 acgcactcct gcgtactgag cgcctcgcgc gcaccttgag cgaccagctg cgcgcggctt
     3541 gcggcgctgc gcgaataaat gctgagcggg tacccggcct tcaggatgaa gccggccatt
     3601 ggcacgccca tgcgtcccgc gccgatccag ccaatgtgcg gcaaagatgc catggtttct
     3661 ccctggttga cttaaggtca tgcccccaga taacaggctt tcacgagctc gctgctgtgc
     3721 agttcgctca tggtacggcc ttcgaacgcg atcgcgcctt gcacgatcag atatccgcgg
     3781 tctgcgatgc ggctggcctg atgaaagttc tgctcggcca tcagcacggt cagcccgatc
     3841 tgccgcttga gcgaggcaat cgtatcgatc atctgcttca ccagcatcgg ggacaaacct
     3901 accgaaggtt catcgaccag taacagcttg ggagccgaca tgagcgcacg cgcgatagca
     3961 agcatttgct gctgcccacc actcaattcg cctgcacgtt gcgcacttcg ctgccccagc
     4021 accgggaatg cttcgtggca cagcgagaga ttgcgcgcga tggcggcgcg tgcgccgctt
     4081 cgatacgcac caagcaacag gttttcggct acggtcagtt tcggaaacag gcgtcgcccc
     4141 tctggtacca gagccacgcc gaggccgacg atttcatgtg gcgttttgcc gaccagatcg
     4201 atgctcacgc cgtcgatttc gaggacgatg gagccgcgcg tgggctttag cagccccatg
     4261 atgcatttca ttaacgtgct tttgccgttg ccgttggtac cgagcaaggc cacggtttcg
     4321 ccgtcgcgca cgtcaagcga gacgccatgc aaaacgcgca cagcgccgta accggcgtcg
     4381 attccctcca tcgcgagttt agtcaccgag gtagatcctc tgcacatcgc ggtcttccac
     4441 gatctgcgcc ggcgcgccgt gcgcgagaat gcggccggcg tccaggcaaa tgactcgctc
     4501 ggagaagcgc atgatcgcac gcatgatgtg ctcgatcatg acaatggcga tgccctcccg
     4561 attgagcgca agcaggatgg caagcatttc atcgacttcg atcgcggaca gtccggccat
     4621 gacttcgtca aggatcagca ggcgcgggcg cgacgccagc gcgcgggcca gttcaagctt
     4681 gcgcaaatcc acctgcgtga gggatgcggg ggaggcatcg gcacggccgc ccagtccgat
     4741 ctgcgccaga atcgcgtggg cttgggcagc gacatcagac cggtgcaggg atttacggtg
     4801 cgtcacatat tccagcggca cagcgatatt ttccgccagc gtcatgctcg caaatggacg
     4861 cggcacctga aaagtgcgcg caatccccag acgcgcgcgc cggtgagcgg gcagcaaagt
     4921 cacgtcagct cccgaaagcc agatcgatcc gctatccgta cccaacgagc cgccgatgca
     4981 attgatcagg gtggacttgc ctgagccgtt cgggccgatg atgccgagcc gctccccgga
     5041 ccgcacggtc aggctaacgt cagccagcgc gacaaaacca ccgaacagtt tgctgaccgc
     5101 gttgactgcc agcaatgcgc cgttcatttg gtgtgctccg ccgaatacca ccggcgcaca
     5161 agtccgagga tgccctcggg cgccgcgacg acgaacgcaa ccagcagcac accaagcacc
     5221 agcacgttga gctccgaaga cagcgttacc gtgaccagct gctggacaga actcagcaat
     5281 accgcgccga tcaccgggcc cgtccagctg gcggtaccac cgatgattgg catcgcgagc
     5341 gcagacaccg catagttgag gctgaatgcc gatgtcggct cgatgaaact catgtagagc
     5401 ggaaacggcg cacccgcaat gcccatcagt gcgccggagg tcgttgcggc gaatagtttc
     5461 aacttcagcg ttggaacgcc cgcggattcg gcggcctcct cgtcgtcgcg cagcgcgcgc
     5521 aagccgcgac cgatccagga tgtttcgatg tagcgcgcca gcaagaccgc actcacggcc
     5581 gccaatgcca tgacgacgaa cagcaggcgc gcgtaggaag aaaacagggc aggcgcggcc
     5641 ggctgcaaca ccgtggcacc gcgcgccccg ccgacgaaac tccaattcag cacaacggtc
     5701 tcgcagatca ccgcgatcgc gaccgtcgca atcgaaaaaa agattccacg caaacgcatg
     5761 gtcatcgcac cgactatcaa tcccatcagg ccactcacca gcgccgcacc ggcaagttga
     5821 agtatcagcg gtgcggcaaa cgccttgatc agcgccagcg cagtgtaggc gccagcggca
     5881 aaaaaggcgc ttgcaccaaa attcacgtaa ccggcatagc cgcccagaat attccaggcc
     5941 gtggccagca caataaactg cagcaccacg tagcctgcga aaaaaaagta ttcgttggaa
     6001 acgatgcgcg ccagcaggag tgcggtcgcg agcagcgcaa ggaccgccag cctgaatttc
     6061 cagggcgtgg gcacgtgttc agcgcccgaa cagcccggcc ggccggatcg ccagcacaag
     6121 caagagcaag ccgaaggcca cggccggcgc ccaggatgcg ccaagtgaag tcagcacgat
     6181 gctttccgta acacccagaa tgactccagc ggcgagcgtg ccgggcatgc tgcccaggcc
     6241 ggcaagcacg gccacacaaa aggttcggcc gatatacagg cgcccgagtc cgggttcgac
     6301 cggcgtgacg atgatcaaca acgcgcccgc gatggcacag gtcgcggtgg caatcgcaaa
     6361 tgcccagtgc ttgatgcgca ccgggtcagc gcccatcagt gcaaccgcgg tctcatcctg
     6421 cgccactgcc ttgatggcac ggccgatgaa tgtgcgcgcg aaaaaaaaag tgagcccgat
     6481 ggtcagcacc agcgccacgc cgaaggcgac cagcatgcgc aacggcaagc gcatgtcgcc
     6541 cagggatacg ctcttgccga tgtagaaagc ctcgaccatg cgctggtcca cgccgaaagt
     6601 gaggatcaac gcaacctcga tgatgaatgc gaggccaaaa aaaaatgcca gtccgcgcaa
     6661 tcccacatcc ttgccgcgac tttcgaagct ggcgtgataa aaacggtaga cggccagtcc
     6721 gcccaggaaa aataagggcg tcagcacaag ccccgcaacc agcggatcga caccgaaccc
     6781 ccccagatag taggcaccgt aggctcccag aaccagaaag gccggatgcg ctatctgcgg
     6841 cacgtcgagc agcccgaacg caacggacag gccaacgctc accgcagcat agaagcagcc
     6901 aagcaatacg ccggcaataa tggcctcgcc gagaagatcg atggacatcc tgtgctttct
     6961 tttggtgctg ttccagcgat taggggcgcg actgattgaa gggcttcaat tcccccgttt
     7021 tgaatgcctc cggtgcaacg atcacttgct tgcccggtgc gcggaactgc tcgagattcc
     7081 tgtccgcgat atcccggaat tgcacataga caatacgcgg attggcccat tctccgcttg
     7141 ggtcatagtg aatgctgccg acaatggttt taatttcgtg tgcgcggatg tatttcgcca
     7201 ataccttatc gtccagactt ctggtggccg tcacagcctg cgccaggatc tggccgattg
     7261 cgtagtcaaa cggcggcagg taaaacccca aaggatcgac attcgcctgg gcggctttcg
     7321 cctgatagcg catcaggaaa tcttttatcc ccggaaagtc catcgttttc tcgggtgccc
     7381 aggtgttgta gttcaccact ccgttcaggg aggaaccaag cgattgcatg atggacgcat
     7441 actgcagacc gaccatgcca ccaccgaaca gcttgacgtt atcgccaatg ccgatctcgg
     7501 cgactgaacg gatgattgca ctggaatccg agggataaga ggcgacgaac acgacatccg
     7561 gatttttggc gcggatggcg cgcacaacgg aggagaactc cacggtgttg ggcggatagt
     7621 tctggtcgta aacggcctcc aggccggatt ccttgaggcc tgaacgcacg ccgcccgcga
     7681 gattttgtgc aaactcggca tccgccgcga taatcgcaat ggtctttgcg ccgactttcc
     7741 tgcacagcaa gagaaacggc gccgcagagt cccgggccga agcccaaggc tggttattga
     7801 aatacttgtc gtgaccaatc ttcgcattcg cgtccagtgc gaagttgccg atcagcagcc
     7861 gatcgcgttg cttgaccagc ggcatgatcg gcgcggtagg attggttgca taaggcgcca
     7921 ccagcaagtc caccttatcg acatccagca acttggaata gagacctggc gtcatcgacg
     7981 ggctgctttg gtcgtcgtag accaccaact caaccgggcg cccaagcaag ccgccggcgg
     8041 cattgacgtc atcgcgccag atctgcagcg caagcagtcc agatttgcct gccccgctta
     8101 gcggtccggt ttgcgcaatg gacaagccga tgcggatcgg cgttttattt tgcgcatagg
     8161 tgcctacctg cgaacagagc aacagtactg cgatgactcc cgcaataccc gctacgatgc
     8221 gccggcctgg gctttgcatt gcaccgctct cctcgttgct ccggcgcaca ttcaacgcgt
     8281 gcgctctggt catcaccaca agaacgacta tagcagcttg cccgcgacac gcaggaccga
     8341 cagcgtcgta gggatgtgga gaagaagcgg gtaaacccgt gatgatcgca gcgaaacttc
     8401 acgggagcgc gcatcgaaaa cgccagttta acggcgtcga atcgcagact tgcgtttcca
     8461 gcgcgtacgg caagtgggac agatgatttc cccggtgact
//
LOCUS       DATMZQ010000524        10880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1640059, whole genome shotgun sequence.
ACCESSION   DATMZQ010000524 DATMZQ010000000
VERSION     DATMZQ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640059"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VN496_16745"
     CDS             <1..593
                     /locus_tag="VN496_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXF18658.1"
                     /translation="GPNLLVWGSSFFIKEPNDPAYISWHQDSTYWGLSHPDIVTAWVA
                     LTVSDVANGAMRVVPGTHLKDQLPHNDTFAENNLLTRGQEVAVEVEDDKAVYLTLQPG
                     EMSLHHVRIVHGSEPNRAHYRRMGFAIRYVPTYVRQTEGPRDYATLVRGRDDFHHFDY
                     EPEPRIDLGAEEVAAHKRITEEANKILYRGTDKVKS"
     gene            613..915
                     /locus_tag="VN496_16750"
     CDS             613..915
                     /locus_tag="VN496_16750"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXF18659.1"
                     /translation="MILEVAILNVIAGREAEFEATFVEAQRIISSMKGYRAHELRRCL
                     EAHNRYILLVHWDKLEDHTIGFRGSAGYQEWKRMLHHFYDPFPVVEHYAPIPGLMD"
     gene            1075..2349
                     /locus_tag="VN496_16755"
     CDS             1075..2349
                     /locus_tag="VN496_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18660.1"
                     /translation="MLTGVLLLIAFLIVVAAIVRGQSPIIMLLLLAIVWALLAGVGIN
                     DLQAKILQGGGVAYASAVIIIVFGAWFAQVLIQTGIAESVIRSAVELTGDRPVVTAMT
                     INLVTALLFTSMYGVGAAIAIGVIALPIMLSQGIPARVAAPVFTMGVGAGAFVNLVQF
                     GTFEKLFPGIKYEAPWLTYWAIGMAVYILIAWLMIWINLRKLGVRHAASIDTAARLPR
                     KRTPYYTYLVPVFPVFMIMVFKWQIIPTFLLSIVLALVLTAKGRSLQGTLNLFNKAFY
                     DAFPDIATIAALWTICGMIIVAGQMPQIAGALRPIFDPVLPHTTLQAAIFFAVLGGIG
                     SIYRGPLVVVGTGAALLAIILSNNEIPVPYLYALWLGPTVMQGSCDPTNSWTLWTIGY
                     TRVPHGQFLKTALPFGCLMVVITCAICYYMFG"
     gene            2363..4522
                     /locus_tag="VN496_16760"
     CDS             2363..4522
                     /locus_tag="VN496_16760"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXF18661.1"
                     /translation="MSERQTYRIGIDVGGTFTKAVLIDNASFQVVGRYSVLTTHSDAR
                     GVAAGVVEVFRNVLERSQVAPNDVVFIAHSTTQATNALLEGDVATVGIIGMASRVESL
                     LARAQSNIKDIELAPGRFLRPAHRFITTEKLDEALVRQTLVGLRDEGAQVVVASSAFG
                     VDDQSAEETVMRLAAELGLAVTGGHEITKLYGLTTRTRTAVINASILPKMIGTANMTE
                     EAVREAGITAPLMIMRGDGGVMDVQEMRRRPVVTMLSGPAASVAGALMYLRVSDGIYF
                     EVGGTSTNIGVIRNGRPTIRHAQVGGHDTYVNSLDVRVIGIAGGSMVRVRGKQIHDVG
                     PRSAHIAGLHYAAFAKADDIVDPRIELFSPRQGDPDDYVAIRCSNGERYALTNTCAAN
                     VLGYTKPGWHAHGNAEAARRAFAALAQHIGLTVEEAARRVLLKSASKITGVVEQLVHE
                     YKLDRDQAVLVGEGGGAASLIPFVGELMQLDFKISQDAEVISSIGVALALVQETIERV
                     MPNPGPEDLQRIKREAFDAVVRLGAAPENVEVTIEVDPQTQRVRATARGASEMRAQDM
                     RKLVSHEEAAHIAAGSMNVAQENVRLAAATDEVRVFQAEVTERKWHMFNKHRRPVRAV
                     DREGVIRIQRSDGVIMQAPAAASLQQLRALWESVTIYNGDSVIAPDMFVIAGRRIVDL
                     CGITVLEQAIAVSRGEIEGLSPETPVVLIGMQGARGL"
     gene            4519..5136
                     /locus_tag="VN496_16765"
     CDS             4519..5136
                     /locus_tag="VN496_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18662.1"
                     /translation="MSERARDRHADPQGSGLLILREDAQYRRIPGRQRVALVQAALDD
                     GRSLADQISSRWGRDPSAIASNCNVPVRRSHEDGGYGGTVFYAEYIARPCGITLYLPA
                     IQKLDRLIAGQGQSYGRTAGTLPIFLAHELYHHFDCQRGNARLSRRHAIPIFSIGAWH
                     WKAGLSSLCEIAAGAFAQQLLGLPFHPKYLDSLVRGDLAARPELS"
     gene            5133..6236
                     /locus_tag="VN496_16770"
     CDS             5133..6236
                     /locus_tag="VN496_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HXF18663.1"
                     /translation="MKFRAAVLDKVNQAMTIDVLDMAPLSATDVLVKIKASGLCHTDL
                     EVIQGSLAYPLPIVLGHEGAGIVEAVGTDVTQVSVGDHVICSWNPHCGHCFYCERDLP
                     ILCEPFVANQPKGFLPDGRSRMKRDGRQVHHYSVTSTHAEYTIVPESGAIPIPHEMPF
                     DRACIIGCGVMTGVGGVVRKAKVEAGTSVLVIGCGAVGLNALQGARLANASIVIAVDI
                     GSIKLRRAVQFGATHAIDGAHETVLDEIKALTGGRGPDYVFEAAGNKRTLRLSVEAVR
                     PGGQVVWLGKINVNEDVSFRWGTLMGEKRIVRSSYGGARPQRDFPWLASEYLKGRLKL
                     DELITQRIRLDQINEGFAALARGEGIRTVIMFD"
     gene            6254..7306
                     /locus_tag="VN496_16775"
     CDS             6254..7306
                     /locus_tag="VN496_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXF18664.1"
                     /translation="MMRFNHPPLLAGQIDAEHRMAVLGDLELESGEILQDYAQSYVTH
                     GSLDTARSNAVLVCISLTGNHHRLDFLIGPGKALDPEYYFIVVADPIGNGLSTSPSNS
                     TYQPGMRFPRFTIRDMVQSQYRLLTRQCGLSRLHAVIGASMGGMQALQWAVSYPEFMS
                     HCVAMTAMARTSAWSVLVTETARCCLMADPAWTGEGFSGAPERGWRAYTGLMTALASR
                     TPEALAELLPDCAAAHLWFEQTYAQNRRIGFDAHDYLYQSWAYEAHDVGTTPDANAET
                     AGALSRIKAKTLLLGPLLDLFNPAQEGRDAAASIPDAQFIEIPSVQGHQAATSTKAED
                     AAFLNRVIAQFLKTTR"
     gene            7418..7888
                     /locus_tag="VN496_16780"
     CDS             7418..7888
                     /locus_tag="VN496_16780"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXF18665.1"
                     /translation="MIDVRALQSTSDAEWVAKVMANSQPWITLSISYERSLALLTNAS
                     RERYLAEVSGQRAGFLMVSMQGAFVGYVQLLGVAAEFRGQGVGKALIEFAEQRIFRET
                     QNVFICVSDFNQDAQRFYARMGYERVGELRDFIVTGHSELLLRKTIGPIRANPK"
     gene            8122..9111
                     /locus_tag="VN496_16785"
     CDS             8122..9111
                     /locus_tag="VN496_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HXF18666.1"
                     /translation="MAGITRRRAGKGWIFFKPDGERVTDAEERRRINALVIPPAWTDV
                     WIAPDSDSHIQATARDARGRKQYRYHTRYRDARDQSKFRHMLEFSEVLPAIRERAERD
                     LRAPKLSRRQILATVVQLLDKTLIRVGNDEYARENRSYGLTTLRMQHVQVNGSKLNFS
                     FRGKSGVQHAISVKDLRIARIVQRCQDLAGQELFQYLNGSGKRETVTSDDVNAYLREI
                     CKRDITAKDFRTWGGTMLAGVELREMGPAATQHQAKRNIIRAIDAVAERLGNTRSVCR
                     KYYVHPALVHAYLQGLVPGPSAIELPEDNRRSRATPALRRDEVAILQFLHDVL"
     gene            9259..9567
                     /locus_tag="VN496_16790"
     CDS             9259..9567
                     /locus_tag="VN496_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18667.1"
                     /translation="MMQEVGNSELTPADIPSFKEVWARIEPFALTFDGFRYWGSVEKC
                     AAVAQARRPGSLTELRTCLFYEARKCQMDGKAPDAIAMRFIRALVYAIREKVDSGDIR
                     "
     gene            9689..10531
                     /locus_tag="VN496_16795"
     CDS             9689..10531
                     /locus_tag="VN496_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXF18668.1"
                     /translation="MRYIAKKVVGVPATDGAGVKLKRVIGSPALDLIDPFLMLDEFGS
                     DDPQSWIAGFPDHPHRGFETVTIMLEGRVKHADSVGNQGVIEAGDVQWMTAGSGIIHS
                     EMPERAQGRLRGFQLWVNLPGKLKMIAPRYQDVQASRIPSVSAADVTVRVIAGEGFGA
                     KGPADTLMPVRLLDVTLHAGAVFKMDVPDDHACFVYVYEGAVRGTDRNGQALEVASGS
                     LGVLEGTAFVTLAAGASGAKLLFADGKPIREPIARYGPFVMNTREEIQKAFEDYSRGE
                     LVTT"
     gene            10561..>10880
                     /locus_tag="VN496_16800"
     CDS             10561..>10880
                     /locus_tag="VN496_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18669.1"
                     /translation="MRQWLLLLGLLLLVPPVLADSQPERLAAAKRYVGVAQIGKITED
                     TVSELAKGFPAEQRTKFLEFMHRAVRPEVLEQAAMESMVKVFTAEELNALADFFGSPI
                     GQSA"
BASE COUNT         2212 a         3077 c         3349 g         2242 t
ORIGIN      
        1 tcggtcccaa tcttctggta tggggcagca gctttttcat caaggaaccg aacgatcccg
       61 cctacatttc ctggcaccag gattcgacct actggggtct ttcgcatccc gatatcgtga
      121 ccgcatgggt cgcgctcacg gtcagtgatg ttgccaatgg cgcaatgcgc gttgttccgg
      181 ggacgcatct caaagaccaa ttacctcata acgatacttt tgccgaaaac aatctgctga
      241 cccgaggaca agaagttgcg gttgaagtcg aggacgataa agcggtgtac ctgacgcttc
      301 agccaggtga aatgtctttg catcatgtgc gcatcgtcca cggctccgag ccgaatcgcg
      361 cgcattatcg tcgcatgggt tttgccatcc gctacgtgcc gacctacgtt cgccagaccg
      421 aagggccgcg ggactacgcg acgttggtgc ggggaaggga tgactttcat cacttcgact
      481 acgagcctga accgcgcatc gacctcggcg ccgaggaagt ggccgcacac aagcgcatca
      541 ccgaagaagc caacaagatt ctgtatcggg gaacagacaa ggttaagtcc tgagtgctcc
      601 cactcgttct ccatgattct tgaagtcgcc attctgaatg tcattgccgg gcgcgaggca
      661 gagtttgaag ccactttcgt tgaggcgcag cggattattt cttccatgaa aggctaccgg
      721 gcgcatgaac tgcgccgctg cctggaagcg cacaaccggt acatactgct cgtgcactgg
      781 gacaagctcg aggaccacac gatcggcttt cgcggttcag ccggctatca ggaatggaaa
      841 cgcatgctgc atcatttcta cgatccgttt ccagtggtgg aacactatgc tccgataccg
      901 ggcttgatgg actgaaatta cctggatggc caacagcagc aagcgaacca tcatggcgca
      961 atgaaacgcc gcagaaaata attctcagct gcgcggtgca gcaccgacaa aatcggaagc
     1021 gttgaataat tgaatacagg ggcggccacg tcctggagtc cataagggga aacaatgctt
     1081 acgggagtat tgctgctgat tgcgttcctg attgtggtgg ccgcgatagt acgcgggcag
     1141 agcccaatca tcatgctgct attgctcgcc atcgtctggg cactgctggc gggtgttggc
     1201 ataaacgacc tccaggccaa gattctgcag ggcggaggcg tcgcctacgc ttccgccgta
     1261 atcatcatcg tgttcggcgc ctggtttgcg caggtgctga tccagaccgg cattgcggaa
     1321 tcggtcattc gttccgcggt cgagctgacc ggcgatcgcc cggttgtgac cgccatgacc
     1381 attaacctgg tgaccgcgtt gctgtttacg tcgatgtacg gtgtgggtgc agcgatcgcc
     1441 atcggcgtca ttgccctgcc gatcatgctg agccagggca ttcccgctcg ggttgccgcg
     1501 cctgtattca cgatgggtgt tggggccggc gcattcgtaa acctggtgca gttcggtact
     1561 tttgagaaac tcttcccggg catcaaatac gaagcgccct ggctcaccta ctgggctatc
     1621 ggcatggccg tctatatcct gatcgcctgg ctgatgatct ggatcaatct gcgcaagctt
     1681 ggtgttcgcc acgccgcctc gattgataca gcggcgcgtt tgccgcgcaa gcgcacgccg
     1741 tactatacct atctggtgcc ggtgttcccg gtattcatga tcatggtatt caagtggcaa
     1801 atcattccga cgttcctgtt gtcgatcgtg ctggccctgg tgcttacagc gaagggacgc
     1861 agcttgcagg gcacgctgaa tctgttcaac aaggcttttt acgatgcctt tccggatatc
     1921 gccaccattg cggccctttg gaccatctgc ggcatgatca tcgtggccgg ccagatgccg
     1981 cagatcgccg gcgcgctgcg gccgatcttc gatcctgtcc tgccgcatac caccttgcaa
     2041 gccgcaatct tttttgccgt attgggtggc attggcagca tctatcgcgg cccgctggtg
     2101 gtggtaggga cgggggcagc gctgctggcg atcattcttt ccaataacga gattcccgtg
     2161 ccttatctgt acgcgctgtg gctcggaccg actgtcatgc agggcagctg cgatccgact
     2221 aattcgtgga cgttgtggac catcggttat acccgagtgc ctcacggcca gttcctgaaa
     2281 accgcactgc cgttcggctg tctgatggtg gtcatcacct gtgctatctg ctattacatg
     2341 ttcggttgag acctatcgat ccatgagcga gcgccagact taccgcattg gcattgacgt
     2401 cggaggaacg ttcaccaagg cagtactgat cgacaatgcc agcttccagg tcgtcggacg
     2461 ctactcggta ttgaccacgc acagcgacgc acgcggcgtc gcggccggcg ttgttgaagt
     2521 gtttcgcaat gtgctggagc gatcgcaggt cgctccaaat gacgttgtgt tcatcgcgca
     2581 ctcaaccacc caggcgacta atgcactgct cgaaggcgat gtagcgacgg ttggcatcat
     2641 cggcatggcg agccgggttg agtcactgct ggcgcgcgcg caaagcaaca tcaaagacat
     2701 tgaacttgcg ccgggacgat tcttgcggcc ggcccatcgc ttcataacga cggaaaaatt
     2761 ggacgaagcg ctggtgcgcc aaacgctggt gggtctgcgt gacgaaggcg cgcaggtggt
     2821 agtggcgagc agcgctttcg gcgtggatga ccagagcgcc gaagagacag tcatgcggct
     2881 cgcggctgaa ctgggcctcg cagtaacggg cggtcacgag atcaccaagc tgtacgggct
     2941 caccacacgc acccgcactg ctgttatcaa tgccagcatc ctgccgaaga tgatcggcac
     3001 ggccaacatg actgaagagg ctgtgcggga agccggcatc acggcgccgc tgatgatcat
     3061 gcgcggtgac ggcggcgtca tggatgtgca ggaaatgcgc aggcgcccgg tggtaaccat
     3121 gctgtcaggt ccggcggcga gcgtagccgg tgcgctcatg tacttgcgag tatccgacgg
     3181 catttatttc gaagttggcg gtaccagcac taacatcggc gtgatccgca acggccgccc
     3241 caccatccga catgcgcagg tcggcggtca cgatacttat gtgaactcgc tcgatgtccg
     3301 cgtcattggc attgccggcg gcagcatggt gcgcgttcgc ggcaagcaga ttcacgatgt
     3361 cgggccgcgc agcgcgcaca tcgctggcct gcactacgcg gcattcgcaa aggcagacga
     3421 tatcgtggat ccgcggattg agttgttcag ccccaggcag ggcgatccgg acgactacgt
     3481 ggcgattcgt tgctcgaatg gcgagcgtta tgcgctaact aatacctgtg cagctaatgt
     3541 actgggttac accaaaccgg gctggcatgc ccatggcaac gccgaagcgg caaggcgtgc
     3601 ttttgcagca ctggcgcaac atattggcct caccgtcgag gaagcagcgc gccgtgtact
     3661 gctgaagtca gcgagcaaaa ttacgggcgt ggttgaacaa ctcgtgcatg aatacaaact
     3721 cgatcgggac caggccgtgt tggtcggtga aggcgggggc gcggcgtccc tgatcccgtt
     3781 cgttggtgag ctgatgcagc tggatttcaa aatatcccag gatgcagaag tgatttcgtc
     3841 catcggggtc gcccttgcgc tggttcagga gaccatcgag cgcgtcatgc cgaatcccgg
     3901 cccggaagac ctccaacgca tcaagcgcga agcgttcgac gcggttgtta gactgggtgc
     3961 tgcgccggag aacgtcgaag tcaccatcga agtcgacccg caaacgcagc gcgtgcgtgc
     4021 tactgccagg ggggcttccg aaatgcgcgc tcaggacatg cgcaagctgg tgagccatga
     4081 ggaggcggca catatcgcag ccggctccat gaacgtggct caggagaatg tccggctggc
     4141 ggctgccacc gatgaagtgc gggtatttca ggcggaagtg accgagcgca agtggcacat
     4201 gttcaacaag caccgccggc ccgtgcgcgc tgtggatcgc gaaggcgtca tacgaattca
     4261 gcgcagcgat ggggtcatca tgcaagcgcc tgctgccgca agcctgcagc agttgcgtgc
     4321 cctgtgggag tcggtgacga tttataacgg cgatagcgtg attgcgccgg acatgttcgt
     4381 catcgcgggt cgccgcatcg tggatctgtg cggcattacg gtgctcgagc aggccattgc
     4441 ggtcagccgg ggcgaaatcg aaggtttgtc gcctgagacg ccggttgtgc tgatcggcat
     4501 gcagggagca cgcgggctgt gagcgagcgc gcacgggacc ggcatgcgga cccgcaagga
     4561 tcaggcttgc tgatcctgcg cgaggacgcg cagtatcggc gcataccggg ccgccagcgc
     4621 gtcgcgctgg tgcaagccgc gcttgacgat ggacgttcac tggcggacca aatcagtagc
     4681 cgatggggac gcgacccgtc ggccattgca tcaaattgca atgtgcccgt gcgccgctct
     4741 cacgaggatg gcggttacgg aggtactgtg ttttacgcgg aatacatcgc gcggccctgc
     4801 ggcatcacgc tttatctgcc ggccatccag aaactggatc ggctaatcgc gggacaaggc
     4861 cagtcctacg gccgcacggc aggcactttg ccgattttcc ttgcacatga gctttaccat
     4921 cattttgact gccagcgcgg taacgcccga ctgagccgcc ggcatgccat cccgatattc
     4981 agcatcggcg catggcactg gaaggcaggt ctttccagtc tctgcgaaat tgccgccggc
     5041 gcctttgcgc agcaattgct tggcctgccg tttcacccga aatatcttga ttcgcttgtc
     5101 cggggcgacc tcgctgcccg gcctgagctc tcatgaagtt tcgtgccgca gtgctggaca
     5161 aggtcaacca ggcgatgacc atcgacgtgc tggacatggc cccgttgtcc gcgaccgatg
     5221 tgctcgtcaa aatcaaagcc agcggtttgt gtcataccga tctcgaagtg atccagggtt
     5281 cgctcgccta tccactgcca attgtgctcg gtcacgaagg agcaggcatc gtggaagcgg
     5341 tcggcacgga tgtcacgcag gtgagcgtcg gagatcacgt aatctgttcc tggaacccgc
     5401 attgcgggca ctgtttctat tgcgagcggg acctgccgat cctgtgcgaa ccgttcgtgg
     5461 cgaaccagcc aaaaggcttc ctgcccgatg gccgatcgag aatgaaacgt gacggccgcc
     5521 aggtgcatca ttattcggtg acttccacgc atgccgaata cactatcgtg cccgaatccg
     5581 gagccatccc gatcccgcat gagatgccct ttgatcgggc ctgcatcatc ggttgcggcg
     5641 tgatgaccgg cgttggcggt gtggtacgca aggcaaaggt ggaggcgggg acaagtgtgc
     5701 tggtaattgg ctgtggcgcg gtcgggctca atgcgctcca gggcgccagg cttgccaatg
     5761 cgtcaatagt tatcgcagtg gacatcggca gcatcaaact gcggcgggcc gtgcaattcg
     5821 gtgcgacaca cgcgatcgac ggcgctcacg aaaccgtgct ggatgaaatc aaagcattga
     5881 ccgggggccg cggaccggac tatgttttcg aggcagccgg caacaagcgg accttgcgcc
     5941 tgtccgtcga ggcggtgcgt ccggggggac aggtcgtgtg gcttggaaaa atcaatgtga
     6001 acgaagacgt cagttttcgc tggggaacgc tgatgggtga aaagcggatc gtgcgctcga
     6061 gttatggagg ggcacgtccg cagcgcgact tcccctggct cgccagcgag tatctgaaag
     6121 gacgtctcaa gctggacgaa ctcatcacac agcgtatccg gctggatcag attaacgaag
     6181 gcttcgccgc gctggcgcgg ggcgagggga ttcgcacagt aatcatgttc gactgagctt
     6241 ctgcttaggc acaatgatgc gcttcaatca tccgccgctg ctggccgggc aaattgacgc
     6301 cgagcatcgc atggcggtgc tcggcgacct ggaactggag tccggcgaga ttttgcagga
     6361 ctacgcgcaa tcctatgtca cgcacgggag cctcgacacg gcaagatcga atgccgtact
     6421 ggtctgtatt tcgctcactg gcaatcacca ccggctcgat tttctgatcg gcccgggaaa
     6481 ggctttggat cctgagtact acttcatcgt agtggccgat ccgattggta atggcttgtc
     6541 gacctcgcct tcgaacagta cgtatcagcc gggcatgcgt tttcctcgct ttacgattcg
     6601 cgacatggtg caaagccagt accgtttgtt gacgcggcaa tgcggccttt cgcgcttgca
     6661 tgcggtcatc ggggcctcga tgggtggcat gcaggcgttg cagtgggcag tgagctatcc
     6721 cgaattcatg tcccactgtg tggcgatgac cgcgatggcg agaacttccg cctggtccgt
     6781 gctggtaacc gagacggcac gctgctgttt gatggcggac ccggcctgga ccggtgaggg
     6841 cttcagcggt gcaccggaac gcggctggcg ggcatacacc ggactgatga cggcgttggc
     6901 gagccggacc ccggaagcgc tggctgaatt gctgccggac tgcgctgcag cgcatctatg
     6961 gtttgagcaa acgtatgcgc aaaaccgcag gatcggattc gatgcacacg actacctgta
     7021 ccagtcgtgg gcttacgaag cgcatgatgt cggtaccact ccggacgcca acgcggaaac
     7081 agctggggcg ctatccagga tcaaggcgaa gaccctgctg ctcggcccgt tgctcgattt
     7141 gttcaatcct gcccaagagg gccgcgatgc cgcggccagc attccggatg cacaattcat
     7201 cgagataccc tcggtgcagg gccaccaggc tgcgacgagt acgaaggcgg aagacgccgc
     7261 ttttctgaac cgcgtaatcg cacagtttct aaaaaccacg cgttagggtt taatgcgacg
     7321 gccacggtgc acgctcacgg cacgcaagcc aggcgtgatc gcgtccggac caccgtgcaa
     7381 atcgcaaagg gagaccgcgc agcagcgctg aagattgatg atagacgtgc gtgccttgca
     7441 atccaccagt gatgccgaat gggtcgcgaa ggtcatggcg aattcgcaac cctggatcac
     7501 gctgtcgatc agctatgaac gcagcctcgc gttgctcacc aatgcgtcgc gggaacgcta
     7561 tcttgcagag gtgtccggac agcgggccgg attcctgatg gtcagcatgc agggcgcctt
     7621 cgttggctat gtccagctgc ttggcgtcgc ggccgaattc cgcgggcagg gcgtcggtaa
     7681 ggcgctgatc gaatttgctg agcaacgcat atttcgtgaa acgcaaaacg tatttatctg
     7741 cgtatcggat ttcaaccagg acgcacagcg gttctacgcc cggatgggct atgaacgtgt
     7801 tggtgagttg cgtgatttca tcgtgactgg ccactccgaa ctgctgctgc gcaagactat
     7861 tgggccgatc cgggcgaatc ccaaatgagc gccttgatac aaatttcacc gcaccacttg
     7921 gcctgcctgg tcggttgatc gccagaccac cgccaacgtt gtggcgccca atgtaacgcc
     7981 ttcgcacttg ctatcaatgc aaaagttggc atatcgtgtg cgccaccgca agccaggagc
     8041 gaacttccga ttccctcgtc gcgcaccata gagcatcgca aggcagccag ccgtgccgga
     8101 cttaactatg tgacggacgg cgtggccggc atcacccgcc gccgggccgg caagggatgg
     8161 atttttttca aacctgacgg cgaacgggtc acggatgcgg aggagcggcg ccggatcaat
     8221 gcactcgtca ttcctccggc atggactgat gtgtggatcg cgccggattc cgacagccat
     8281 atacaagcca cggcgcgcga tgcccggggc aggaaacagt accgttacca tacgcgctat
     8341 cgcgatgcgc gcgatcaatc gaaatttcgg cacatgctgg aattcagcga ggtgctgccg
     8401 gccatccggg agcgtgccga acgggacttg cgcgcgccga aattgagccg gcgccagatt
     8461 ctcgccacgg tcgtgcaact gctggacaaa accttgatcc gtgtcggcaa cgatgagtat
     8521 gcgcgcgaaa accgttccta tggcctgaca acgctgcgca tgcagcacgt ccaggtcaac
     8581 ggttcgaaac tgaatttcag ttttcgcggc aagagcggcg tgcagcatgc gatatcggtg
     8641 aaggacctgc gtatcgcacg catcgtgcaa cgctgccagg atctcgccgg acaggaatta
     8701 ttccagtacc tgaacgggtc cgggaagagg gaaaccgtga cttcggatga cgtaaacgct
     8761 tatctacgcg aaatctgcaa gcgcgatatc acggcgaagg attttcggac ttggggaggt
     8821 acgatgctgg caggagtgga gttgcgcgag atgggcccgg cagccacgca gcaccaggcc
     8881 aagcgcaaca tcattcgtgc aatcgatgcc gtagccgagc ggcttggcaa tacgcggtcg
     8941 gtctgccgca agtattatgt acaccccgcg ctggtccatg cttatctgca aggtcttgtc
     9001 ccgggcccgt ccgcgattga attgcccgag gacaatcgcc gtagcagggc aacaccggcc
     9061 ttgcgacgcg acgaagtagc cattctgcaa ttcctgcacg atgtcttgtg atgggcgagc
     9121 gctttctggt gccgcttgtc accccggcgc gacaatacgg tccgatgcgg aagaatatgt
     9181 cattgatatt aagcggatga cgattccggc gtaatttttg cgtcggcctt cgacgttcca
     9241 atctcgagtg gcatatccat gatgcaggaa gttggcaatt ccgaactcac cccggccgac
     9301 atcccttcat tcaaggaagt ttgggcgcgc atcgagcctt tcgcactgac ttttgacggc
     9361 ttcaggtact ggggatcggt cgaaaaatgc gccgccgtgg cgcaagcccg gcgcccgggg
     9421 tcgttgaccg aactgcggac ctgcctgttt tacgaggcgc gcaaatgcca gatggacggc
     9481 aaggcgccgg atgcgatcgc aatgagattc atccgtgcac tcgtatatgc gattcgcgaa
     9541 aaagtcgaca gcggggacat tcgctaacgc atagctcctg cactttcgga cgggatagac
     9601 gggcgggaca gctgcggctt gaaaatcaga acgccggccg cacatggtat aagaccgttg
     9661 tccaacggac cttatcccgg agaaacaaat gcgatacatc gccaagaaag tcgtgggcgt
     9721 acccgctacg gacggtgccg gcgtgaaact caagcgtgtc atcggcagtc ccgcgctgga
     9781 tctgattgac ccttttctga tgctggacga atttggttcc gacgatccgc aatcctggat
     9841 cgccggcttt ccggatcatc cgcatcgcgg ctttgaaacg gtcaccatca tgctggaagg
     9901 tcgcgtaaag cacgccgact cggtgggcaa tcagggcgtc atcgaggccg gcgacgttca
     9961 atggatgact gccggcagcg gaatcatcca ttcggaaatg ccagagcgtg cgcaaggcag
    10021 attgcgtggt tttcagctgt gggtcaattt gccgggcaaa ctgaagatga tcgcgcctcg
    10081 ctatcaggat gtgcaggcgt cgcgcatccc gtccgtatcg gccgctgatg taaccgtgcg
    10141 tgtcattgcc ggggaaggct ttggagcaaa gggtccggcg gatacgttga tgcccgtccg
    10201 cctgctagac gtgacactgc acgccggcgc tgttttcaaa atggatgttc ccgacgatca
    10261 cgcctgtttc gtctatgtct atgaaggcgc cgtacgcggt accgaccgca atggccaagc
    10321 actggaagtg gcgagcggtt ctcttggcgt actcgagggg acagcgttcg ttacgctggc
    10381 tgcgggtgca tcgggtgcaa aacttctgtt cgccgacggc aaaccaatcc gtgaacctat
    10441 tgcccgctat ggccccttcg tgatgaacac gcgtgaagaa atccagaaag ctttcgagga
    10501 ttattcacgc ggcgaactgg taaccacgta attgtgaatt caaaaaggga aacgatgacc
    10561 atgagacagt ggctcttgtt gcttggcttg ctgttgcttg tgccgcccgt gctggcagac
    10621 tcgcagccgg agcggcttgc cgccgccaag cgctacgttg gcgtggcgca gatcggcaaa
    10681 attacggagg acaccgtctc ggaacttgcc aagggctttc cggccgagca gcgcacgaaa
    10741 tttctggaat tcatgcatcg cgccgtgcgg ccggaggtgc tggaacaggc ggccatggaa
    10801 agtatggtca aggttttcac cgcggaagag ttgaacgcgc tggctgattt tttcggctcg
    10861 cccattggcc agtcggcaat
//
LOCUS       DATMZQ010000525         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2339975, whole genome shotgun sequence.
ACCESSION   DATMZQ010000525 DATMZQ010000000
VERSION     DATMZQ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339975"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1150
                     /locus_tag="VN496_16805"
     CDS             122..1150
                     /locus_tag="VN496_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HXF18670.1"
                     /translation="MKVLVTGVAGFIGMHVADRLLARGDEIIGVDNVNAYYDVALKEA
                     RLDRLRKQSGFRFVKADIADADALGQVFQREKIQSVIHLAAQAGVRYSLDNPMAYVQS
                     NLVGFVNVLEGCRHSNVEHLVYASSSSIYGGNQKIPFSEHDNADHPISLYAATKKSNE
                     LMAHTYSHLFGLPTTGLRFFTVYGPWGRPDMSYFKFTRAILEGRPIEVFNHGKMQRDF
                     TYVDDIAESVVLVLDRPPQAQPGFDAINPDPASSSAPYRIYNIGNHRPIDLMTYINVL
                     ERALGREAEKTLLPLQDGDVVCTYADTAALQAHIEYVPTTSVEDGIRRFVDWYCAYYG
                     VKRPAGLK"
     gene            1147..1917
                     /locus_tag="VN496_16810"
     CDS             1147..1917
                     /locus_tag="VN496_16810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXF18671.1"
                     /translation="MNISAVVIAKDEEAAIGACLDSVSWCKEIILVDSGSQDRTREIA
                     AARGAKVHEHVWEGYGRQKNFAVRQATNDWVLCIDADETISPALRASIEKTLTDPAHR
                     VYEMARCNRFMGVWLRHGEGYPDWILRLFDRRVARWSDDPVHEKVETDEQVGRLKGDL
                     LHTSAETLHSYLEKQNTYTSLQAQLLYRQGKRANAAKLVLSPLYRFLKFYVLRCGFMD
                     GVPGLVHVSIGCFNSFLKSAKLSALWREAERPPTAARR"
BASE COUNT          477 a          580 c          619 g          495 t
ORIGIN      
        1 tcgtgtggca atcaattggc tgctttgatg ttccgtcgag ttgcgcctct gcgacaccgg
       61 tgtgatgatg ctgctgaact tgcgacacta actgaatcat tatccgaaac gctgccaaat
      121 aatgaaagta cttgtaacgg gtgtcgcagg tttcatcggc atgcacgtgg ccgatcggct
      181 gctcgcgcgc ggcgatgaaa taatcggcgt agacaatgtt aatgcctatt acgatgtagc
      241 tctgaaggaa gcgcgcctgg accgattgag aaaacagtcc ggctttcgat tcgtcaaggc
      301 cgacatcgca gacgcggatg cattgggcca agtgttccag cgggaaaaaa tccagagcgt
      361 gatccatctt gccgcccagg cgggtgtgcg ttattcgctg gataacccga tggcatacgt
      421 gcaaagcaac ctggtaggtt ttgtcaacgt tctggaaggt tgtcgccata gcaatgttga
      481 acaccttgtc tacgcgagca gttccagcat ttatggcggc aaccagaaaa tacctttttc
      541 agagcatgat aatgccgatc atccgataag cctgtacgcg gccaccaaaa aatcgaatga
      601 gttgatggcg cacacctata gccatttgtt cggtttgccg actaccggcc tgcgtttctt
      661 cacggtatac gggccatggg gccgccccga catgtcttat ttcaagttca ccagggcgat
      721 cctcgaagga cggcccatcg aggttttcaa tcacggcaag atgcagcggg atttcacgta
      781 tgtcgacgat atcgccgaga gcgtggtgct ggtcctggac cggccgccac aggcgcaacc
      841 cggcttcgac gcaatcaatc ccgaccccgc atccagcagc gcgccctacc gcatttacaa
      901 tatcggcaat catcgtccaa ttgacttgat gacttacatc aacgtgctcg aacgggcact
      961 cggacgggag gcggaaaaga cgctgcttcc tttgcaggac ggggatgttg tgtgcactta
     1021 tgccgacacg gctgccttgc aggcccacat cgagtatgtt ccgacaactt cggtagaaga
     1081 tggcatccgg cgctttgtag actggtattg cgcttactac ggcgtaaaaa ggccggctgg
     1141 tctcaaatga atatttccgc ggtggtcatt gccaaggacg aggaggccgc gataggggca
     1201 tgcctggatt ccgtttcgtg gtgcaaggaa atcatccttg tcgactctgg aagccaggac
     1261 cggacgcgtg aaatcgctgc tgcacgcggt gccaaagtac acgagcacgt atgggaaggc
     1321 tatggcagac agaaaaactt cgctgtccgc caggcgacca atgactgggt gctgtgcatc
     1381 gacgccgacg aaaccatcag ccccgcgctg cgcgcgagta tcgaaaagac attgaccgat
     1441 cccgcacatc gcgtctatga aatggcgcgt tgtaatcgct tcatgggtgt ctggctgcgc
     1501 catggtgagg gctatccgga ctggattttg agattgttcg accgccgagt cgcgcgctgg
     1561 tcggatgatc cggttcatga aaaagttgag acggatgaac aagtcggacg tctgaagggc
     1621 gatcttctgc atacttcggc cgagacgctg cattcgtatc tggaaaagca gaatacctac
     1681 acgagtctgc aggcacagct cttgtatcgt cagggaaaac gagcaaacgc ggcaaagctg
     1741 gtactgtcgc cgctgtaccg gtttctgaag ttctacgtac tgcggtgcgg attcatggat
     1801 ggcgtgccag gtctggtcca tgtgtcgatc gggtgcttca acagcttctt gaaaagcgcc
     1861 aagctgtcag ctctttggcg agaagccgaa cgtccaccca cggcggctcg tcgttgaaga
     1921 gtttccatcc cgtctcagtt taggctgggc ggctataatt cggcctggtc ccgcaacgcc
     1981 ttttgacttc cgccgtcaca agcgcaactg aattcctgtg agtttttccg aagtctttct
     2041 tccaccagac gcggtcgcgt tggaccagat ccagcgcgtg ctggtcatca aattgcgcaa
     2101 cctcggcgat gttttgctgg catcccccgt cgtgtccgcg ttgaaggcgc agttgcctca
     2161 cgtggaggtc g
//
LOCUS       DATMZQ010000526         5532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2340586, whole genome shotgun sequence.
ACCESSION   DATMZQ010000526 DATMZQ010000000
VERSION     DATMZQ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340586"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VN496_16815"
     CDS             complement(<1..660)
                     /locus_tag="VN496_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXF18672.1"
                     /translation="MRKLWLIFAQAVTISLAALFVVVTLRPDLVPWTGHGNGIVTVRE
                     TSEGPHERVNSGSFSGAARKAMPSVVNIFTSKDVKVPRHPFMDDPLFRRFFGDQLDGE
                     TQHSSSLGSGVIIGPEGYILTNHHVVEAADEIEVALADGRKTPAKVVGTDPETDLAVL
                     KANLSGLPAVTFGNPDRLSVGDVVLAIGNPFGVGQTVTMGIVSALGRSHLGISTFENF
                     IQ"
     gene            900..1328
                     /gene="rplM"
                     /locus_tag="VN496_16820"
     CDS             900..1328
                     /gene="rplM"
                     /locus_tag="VN496_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXF18673.1"
                     /translation="MKTFSAKSHEVKRDWFLVDATDKVLGRLASEIARRLRGKHKVTF
                     TPHIDTGDYIVVVNAEKLRVTGAKAEQKVYYRHSTYPGGIYSATFSRLHNRFPGRVLE
                     KAVKGMLPKGPLGYAMLKKLKVYAGSDHPHSAQQPQNLNV"
     gene            1337..1729
                     /gene="rpsI"
                     /locus_tag="VN496_16825"
     CDS             1337..1729
                     /gene="rpsI"
                     /locus_tag="VN496_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXF18674.1"
                     /translation="MAIQYNYGTGRRKSAVARVFLRPGSGKIIVNAKPVDEFFSRETG
                     RMVVRQPLVLTNHLGTFDISINVDGGGESGQAGAVRHGISRALVDYDASLKPVLSKAG
                     LITRDAREVERKKVGLHKARRRKQFSKR"
     gene            1833..2861
                     /gene="argC"
                     /locus_tag="VN496_16830"
     CDS             1833..2861
                     /gene="argC"
                     /locus_tag="VN496_16830"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXF18675.1"
                     /translation="MKKIGIVGGTGYTGVELLRILATHPEVELKTITSRKEAGTAVAD
                     MFPSLRRRVRLDFSDPSTSSLEKCDIVFFATPNRVAMTQAPALLEAGVRVIDLSADFR
                     IKDIGEWEKWYGASHSSPELVSNAVYGLPEVNRLQIVDARLVANPGCYATAIQLGFLP
                     LVEAGVVDNAQLIADAKSGVSGAGRKAEVHLLFSEASDIFKAYAVSGHRHLAEITQEL
                     SRAAGQKLGLTFVPHLTPAIRGIHATLYARLTRQIDVQALYEKRYSGEFFIDVLPPNS
                     HPDTRSVRGANDCRIALHRPGGGDVVVILSVLDNLVKGASGQAVQNMNIMLGLPESLG
                     IEHVPLLP"
     gene            2858..3601
                     /locus_tag="VN496_16835"
     CDS             2858..3601
                     /locus_tag="VN496_16835"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HXF18676.1"
                     /translation="MTLIRTLKRRFGIAAPRVAIHTHIPWYWRWVSIIAFAASIVSIG
                     WLTYDFGRQFAGFDNSEAQREKNNLEQQITKLQGENSALRGEVAASERQLEIEFATHK
                     NLSGQIQNLSEENALLKEDLAFFQTLMASGSEAGGISVNRFRVQPDALPGEYRYRLLI
                     AQSKQRVKDFKGRLQFIVDFEQDGKSAVISFPTEDNKTQAYNLAFKFYQRVEGTLLVP
                     AGAVVKRVQVRVLETGNPTPVSTQTVTLS"
     gene            3614..4021
                     /locus_tag="VN496_16840"
     CDS             3614..4021
                     /locus_tag="VN496_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXF18677.1"
                     /translation="MFGAKPTKPQNRIDSLIGAATVVEGNISFSGGLRVDGSVRGNIS
                     TSDDQPSTLVLSEKAQIEGEIRVSHAVINGTVIGPVHGAEYVELQPKSNVTGDVHYRT
                     LEIQLGAVVQGRLVHQDDDKAQKIVALKSSGSE"
     gene            4071..4421
                     /gene="erpA"
                     /locus_tag="VN496_16845"
     CDS             4071..4421
                     /gene="erpA"
                     /locus_tag="VN496_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HXF18678.1"
                     /translation="MNAVTEMPAPLLFTDNAAKKVKELIDEEGNPGLKLRVFVTGGGC
                     SGFQYGFTFDEDINEDDASMEKNGVTLLIDAMSYQYLVGAEIDYQESIEGSQFVIKNP
                     NASSTCGCGSSFSA"
     gene            complement(4450..>5532)
                     /locus_tag="VN496_16850"
     CDS             complement(4450..>5532)
                     /locus_tag="VN496_16850"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HXF18679.1"
                     /translation="YIGLMSGTSLDGVDAVLVDFSAAVTRRCIVTESFTSFDASLRRR
                     LILLHQTQTDELHEAALLGNELARIYAQAVHTLLAKSHQRTLDVQAIGCHGQTIRHRP
                     DARYTIQLGNGALLAELTGKIVVCDFRSRDIAAGGQGAPLVPAFHDAVFANPSTHRVI
                     LNIGGIANVTDLSNGPVRGFDTGPGNMLLDAWIERHLGLAFDRDGAWAEGGKCIDALA
                     RRLLADGFFHREPPKSTGRELFNLSWLDGQLSGNENPQDVQATLLELTVVSIARAITA
                     WCNGAKEIYVCGGGASNKALMSRLTAAMPNALVATTAQLGIGPDWVEACAFAWLARQA
                     LRLQPANLPSVTGATGPRILGAIYPG"
BASE COUNT         1318 a         1414 c         1561 g         1239 t
ORIGIN      
        1 ctggatgaag ttctcgaacg tgctgatgcc aagatgactg cgccccagcg cgctgacgat
       61 tcccatcgta acggtctggc cgacaccgaa tggattgccg atcgcaagca caacgtcgcc
      121 aacggacagc ctgtccggat tgccgaatgt gactgcggga agcccggaaa gattggcttt
      181 tagaacggcc aggtctgtct ccgggtccgt cccgaccacc ttggcgggcg tttttcgtcc
      241 atcggccagt gcgacttcta tctcgtcggc cgcctcgacg acgtgatggt tggtcaagat
      301 gtagccttcg gggccgatga ttacgccgga ccccaagctg gaactgtgtt gggtttcgcc
      361 gtcgagttga tcgccaaaga aacgacgaaa aagtggatcg tccatgaacg gatggcgcgg
      421 gactttcacg tccttgctgg taaaaatatt gacgaccgag ggcatagctt tcctggccgc
      481 tccgctgaac gacccggaat ttacgcgttc gtgtggacct tccgaggttt ctcgtaccgt
      541 tacgataccg ttgccgtgtc ccgtccatgg cacgagatcc gggcgcagag tcactacgac
      601 aaacagggct gctaggctga tcgtgacggc ctgagcaaat atgagccaaa gcttgcgcat
      661 aaatgaacgg aaggaagcgc caggtggctg cgccgggaac gcattattca ccaaatggct
      721 ttgtcattga agtacagtcc acacaaatgg atatagcaac ggcgcgctgc atcacttgat
      781 gcagaaccgt tttacttttt atattccgtt acttgcagac aaaatcgtgg tttgacaatg
      841 gacacagcgt tcaactacac tacgcggctt ttgttaagcc gacttcggac cctccgtcca
      901 tgaaaacgtt ttctgccaaa tcacacgaag taaagcgcga ctggtttctg gttgacgcca
      961 cggataaggt gctgggacgt cttgcgtccg agatcgccag gcgactgcgt ggcaagcaca
     1021 aagtcacttt cactccgcac atcgacacag gcgattacat cgtagtcgtt aacgcagaaa
     1081 aactgcgcgt aaccggcgcc aaggcggaac agaaagtgta ttaccggcac tccacctatc
     1141 caggcggaat ctacagtgcg accttttcaa gattgcataa ccgtttcccg gggcgtgtgc
     1201 tggaaaaggc agtgaagggc atgctgccca aaggtccact gggttacgcg atgctaaaga
     1261 aactgaaggt ctatgcgggg tcggatcacc cgcactcggc acagcaaccg cagaacctca
     1321 acgtttagga aattaaatgg cgattcagta taactacggg accggccgcc gcaaaagtgc
     1381 ggtggcccgt gtcttcctca gaccgggcag tggcaagatt attgtcaatg ccaaaccggt
     1441 ggacgaattt ttctctcggg agaccggacg catggtcgtt cggcagcccc tggtgctgac
     1501 taatcatctg ggcactttcg atatttcgat aaatgtggat ggtggtggag aatcgggaca
     1561 agcgggcgct gtgcggcatg gcatttcacg ggcgctggtt gattacgatg cgtcgcttaa
     1621 gccggttctg agcaaggcgg gcttgattac acgggacgca agagaagtcg aacgaaaaaa
     1681 agtgggtctg cacaaggcgc gacgtcgtaa gcagttttca aagcgttgag atgccctccg
     1741 gccatcgaaa gccgccttcg ggcggctttt ttatttgctt cacgctcttg gcaggcgata
     1801 tcatcaatga atatgcataa gtcgttggta agatgaaaaa aataggcatc gtaggcggga
     1861 caggctatac cggcgtagaa ctgcttcgga tactcgctac acatccagaa gtggaactaa
     1921 agacaattac ctcgcgcaaa gaggcgggga cggcggtggc cgatatgttt ccaagcttgc
     1981 gccggcgagt ccgtcttgat ttttccgatc cgtccacaag ttcgctggaa aaatgcgaca
     2041 tcgtgttttt tgccacgcca aacagagtgg ccatgacgca agctcccgcg ctacttgagg
     2101 ccggcgttcg tgtaatcgat ctttccgcag acttccgcat caaggacatt ggcgaatggg
     2161 aaaagtggta tggcgccagt cattcttctc ctgaactcgt atcgaatgca gtgtacggtc
     2221 tgccggaagt aaatcgcctt cagattgtgg acgcacgatt ggtggccaat cctggctgtt
     2281 atgcgacagc tattcaactt ggtttcctgc cattggtcga ggcgggggta gtggacaatg
     2341 ctcaattgat tgccgatgca aaatctggag taagcggggc gggacgcaag gcggaggttc
     2401 atctgctttt ttcagaggct tccgacatct tcaaggcgta cgcggtatcc ggtcaccggc
     2461 acctggccga aatcactcag gagttgagcc gcgcggccgg acaaaagctc ggattgacgt
     2521 tcgtaccaca cctgacgccg gccatacgcg gtattcatgc gacgctgtat gcaaggctta
     2581 ctcggcaaat tgatgtgcag gcgctctacg aaaagcgcta ctcgggcgaa ttctttattg
     2641 acgttctgcc acccaatagt catccggata cgcgttcggt aaggggtgcc aatgactgca
     2701 gaatcgcgct gcatcgcccc ggtggcgggg acgtggtagt gatactctcg gttctggaca
     2761 acctggtgaa aggcgcatcc gggcaagccg tacagaacat gaatatcatg ctaggactac
     2821 ccgaatccct cggcattgag cacgttccgc tgttgccgtg acgttaatca ggacactcaa
     2881 acgccgcttc ggtatcgccg ctccacgagt ggctatccac actcatatac cttggtattg
     2941 gcgatgggtt tcgatcatcg cctttgcggc atccatcgtg agtatcggtt ggctaaccta
     3001 cgactttggc cggcaattcg cgggattcga caatagcgag gcgcagcgcg agaaaaataa
     3061 tctggagcag cagattacaa aactgcaggg ggaaaattct gctttgcggg gtgaagtcgc
     3121 tgcatctgaa cgtcaattag agattgagtt cgccacacat aagaatctgt caggccaaat
     3181 ccagaatttg tcagaggaga acgcgctcct gaaagaagat ctggctttct tccaaacgct
     3241 aatggcttct ggctcggagg ctggtggtat cagcgttaac cggtttcgtg tacaacccga
     3301 cgctttgccg ggcgaatacc gttatcgatt gttgattgct caatcaaagc agcgcgtaaa
     3361 ggatttcaag ggacgactgc agttcatagt ggattttgaa caggacggca aatcagcagt
     3421 catcagtttt ccgactgagg acaacaaaac ccaggcgtat aatctggcgt tcaagtttta
     3481 tcagcgcgtc gaagggactt tgttggttcc tgctggcgct gtcgtcaaaa gggtacaggt
     3541 tcgcgtgctg gaaactggca atccaacgcc cgtctcgacc cagaccgtca ccctgtcgta
     3601 agcggagact caaatgtttg gagccaaacc caccaaaccg cagaatcgca ttgatagtct
     3661 gatcggggcg gccaccgttg ttgagggaaa tatttcgttc agcggggggc tgcgtgttga
     3721 tggcagcgta cggggaaaca tttcaacgtc cgatgatcaa cccagcacct tggttttgag
     3781 tgaaaaggcc caaattgagg gggaaatacg ggtttcccac gcggtcatca atggaaccgt
     3841 cattgggcct gtacatggtg ccgaatatgt agaactgcag ccgaagtcga atgtcacagg
     3901 agacgtgcat taccgaaccc tggagattca attgggcgcc gttgtacagg gcagactggt
     3961 tcaccaggac gacgacaaag cgcaaaaaat cgtggcgctg aagagttctg ggagcgaata
     4021 acggtttatc agcctagaat ggtgcaagtc aggtactttg ggagtatcga atgaatgcag
     4081 ttacggaaat gccggcgccg ctgttgttta ccgacaacgc cgccaaaaag gtgaaggagt
     4141 tgatcgacga ggaaggaaat cccggcctga agttgcgagt tttcgtaact ggtggaggtt
     4201 gctcgggatt tcagtacgga tttacttttg atgaagacat caatgaagac gatgcgtcga
     4261 tggaaaagaa tggtgtgacg ctattgatcg atgcgatgag ttatcaatat ctggtgggtg
     4321 cggagatcga ctaccaggaa agtattgagg gatcgcaatt cgtgataaag aatcccaatg
     4381 caagttctac ttgtggatgc ggctcctcgt tttccgcctg agcgcttgaa tttttccttg
     4441 cactgattct caacccggat agatcgcacc gagtatccga gggccagtcg ctccagttac
     4501 tgacggaaga ttggcaggct gcaagcgcag tgcctggcga gccagccagg caaacgcgca
     4561 agcctccacc caatctggtc cgatcccgag ttgcgcggtt gtcgcaacta acgcgttcgg
     4621 catcgctgcc gtgagtcgcg acatcaatgc cttgttcgaa gcgcctccac cgcacacata
     4681 aatttcttta gcgccgttgc accacgccgt gatcgctctg gcgatactaa caaccgtcaa
     4741 ttcaagaagc gttgcttgaa cgtcctgcgg gttctcgttt ccggacaatt gcccatcgag
     4801 ccaggacagg ttgaagagtt cgcgtccggt gctctttggt ggctcccggt ggaaaaaacc
     4861 atctgcaaga agccttcggg ccagagcgtc gatacacttg cctccttcag cccacgcgcc
     4921 gtcccggtcg aaggccaagc ccaaatggcg ttctatccag gcatccaata gcatattgcc
     4981 gggtcccgta tcgaaccctc gcactggtcc attggaaaga tcggtcacat ttgctatacc
     5041 tccaatattc aggatgactc gatgtgtcga cgggttagca aacacggcgt catggaatgc
     5101 cggaaccagt ggtgccccct gaccgccagc ggcaatgtct cggctacgga aatcgcaaac
     5161 aacaatcttt ccggtcagtt ccgcaagtaa cgctccatta ccaagctgga tggtgtacct
     5221 ggcatctggc cggtggcgga tggtctgacc atgacagcct atggcttgaa cgtcgagggt
     5281 tctttggtgc gatttggcga gcaaggtatg aacagcttgc gcatagatgc gcgcgagctc
     5341 attcccgagc aatgcggcct catgtagttc gtcagtttgt gtctgatgaa gaagaatcag
     5401 ccgtctgcgc aatgaagcgt caaaggacgt gaaggactcg gtgacaatac agcggcgggt
     5461 aactgcggcg gaaaaatcaa cgaggacggc atccacgcca tccagactcg ttcccgacat
     5521 caagccaatg ta
//
LOCUS       DATMZQ010000527         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2347006, whole genome shotgun sequence.
ACCESSION   DATMZQ010000527 DATMZQ010000000
VERSION     DATMZQ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347006"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1687)
                     /locus_tag="VN496_16855"
     CDS             complement(95..1687)
                     /locus_tag="VN496_16855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXF18680.1"
                     /translation="MHLRCLHCHDKIDVAHDHSLDGVTCPSCGGQFNLISGETVTHGQ
                     GHASTSTNAPVRSVAHFHLLEQIGVGAFGVVWKARDTHLGRTVAVKLPRRGQLEPSEV
                     EFFLRDARAAAQLRHPNVVAVHEVGHDNGSVYIVSDFIEGVNLKQWLTDQQLSLMEAV
                     ELLIRIAEAVQHAHDHGVIHRDLKPANILMDRDGQPHVADFGLAKHEAGEVTMTVSGQ
                     IMGTPAYMSPEQAEGKAHQADARSDIYSLGVILFELLTDALPFRGQSRMLIVQILRDE
                     PPSPRKLNARIPRDLETITLKCLEKDPAGRYQTSEELADELRRYSAGKPIHARPINRA
                     EKLWRWSCRNPVLAALATVVIALTTTVAAISSGSWLLLRRETNLRHAAIADGLVRRLM
                     EVPTNQVPALLPQLDEYRDRVDPLLQQVAKNHESTPRERLHVSMALVKQRLVGSDILV
                     DTLEILPADAVAPVRDVLTPFASEVSGTLWKKLAAENVLAEPRLRYACLLASYDPANP
                     TWDKLADVVCDDLRMQDIASLS"
     gene            2268..>2799
                     /locus_tag="VN496_16860"
     CDS             2268..>2799
                     /locus_tag="VN496_16860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXF18681.1"
                     /translation="MNDVTQILTAIERGDPQASEQLLPLVYSELRKLAAQRLAQEKPG
                     QTLQATALVHDAYVRLVDADQLAHWNSRGHFFAAAAEAMRRILVEQARRKRRIKHGGG
                     RRRVDLDSAISMTEPPSQDLLALDDALSKLAAIDSAKSDLVKLRFFAGLTMPEAAAAL
                     GISLATAERHWTFARSW"
BASE COUNT          547 a          833 c          811 g          608 t
ORIGIN      
        1 ccgcactatg tcgcttcttc atggcgaatc ctttccggcc caactggccg gctggattct
       61 ctcatattcc ctggatcagt tttcgggaag ggggtcaact aaggctggcg atgtcttgca
      121 tccttaagtc gtcgcagacg acgtccgcca gtttgtccca agttgggttg gcgggatcgt
      181 acgaggccag caggcacgca tatcgaagcc gtggctctgc caagacgttt tcggctgcta
      241 gtttcttcca cagcgtgcct gacacttcgg aagcaaatgg cgtaagaaca tctcgcacgg
      301 gcgccacagc atctgccgga agaatctcca atgtgtccac aagaatgtcg cttcccacga
      361 gccgttgctt cacgagggcc atggacacgt gaagccgctc tcgcggggtc gattcgtgat
      421 tctttgcgac ttgctggagc aatgggtcta cacggtcacg gtattcatca agctgaggca
      481 gcaaggccgg cacctggttc gtgggcacct ccatcagtcg gcgtaccagc ccgtcggcga
      541 tcgctgcatg ccgcagattc gtttcgcgac gcaacaagag ccagctgccg cttgaaatgg
      601 ctgcaaccgt ggtcgtcagc gcgatgacca cggttgccaa cgctgccaga accggattcc
      661 gacacgacca tcgccagagc ttttctgctc ggttgatcgg ccgagcatga atgggtttgc
      721 ctgccgaata gcgacgcagc tcgtcagcca gctcttcgga cgtctgatag cgacctgctg
      781 gatctttttc caggcacttg agtgtgatgg tttccaagtc gcgtggaatt ctcgcgttta
      841 gctttcgcgg gctgggtggc tcatcgcgca ggatttgcac gatcagcatt cgcgattgac
      901 cgcggaacgg caatgcgtcg gtcagaagct caaacaggat tacgccaaga gaatagatgt
      961 cgctgcgggc gtctgcttga tgtgccttgc cctcggcttg ctcgggagac atgtaggcgg
     1021 gggtgcccat gatctgcccg ctgacggtca tggtgacttc gccggcctca tgcttggcca
     1081 gaccaaagtc tgcaacgtga ggctgaccat cccgatccat caggatgttg gcaggcttga
     1141 gatcgcgatg aatcactccg tgatcgtgag catgttggac tgcctcggca atcctgatca
     1201 acaattccac tgcctccatg agactgagct gttggtcggt aagccattgt tttaagttga
     1261 cgccttcgat gaagtcgctg acgatgtaga cagagccgtt gtcgtgcccc acctcgtgaa
     1321 ccgctaccac gttgggatgc cgcagttgag ccgctgcacg agcatcgcga agaaagaact
     1381 cgacttcgga cggctcaagc tgcccgcggc gaggcagctt tactgccacc gtgcggccta
     1441 agtgcgtgtc ccgcgccttc cagacaactc caaacgcgcc cactccaatc tgctccagca
     1501 gatgaaagtg cgccacggaa cgcaccggcg cattcgtcga tgtcgaagca tgcccttggc
     1561 catgcgttac ggtttcgcca ctgatcagat tgaactggcc gccgcacgac ggacaggtta
     1621 ctccgtccag agaatggtcg tgggccacgt cgatcttgtc atggcaatgc aggcatcgca
     1681 gatgcatgac agacgaagcc tcggcctgac gttactagga gcggtccgac gcccaacatt
     1741 atgggtattg agcaatattg cttcaacttt ctgtcggatg cgagcacctt cttggcgaat
     1801 tctaatgtcg gcgggcctga tggacgacgc gccgactttc gcgtgcggtg ctgtttccgt
     1861 gcgttcgcaa cgactccctg gcttggccgt atcgcgccgg agcaatcctt gtgctggcga
     1921 ggctggatcg ctgaacaaca gcagtcaagg ggcatcgctg ccggaccgtt tgaaatgcca
     1981 agaccgacgc gagtagtccg ttctggccct atttcatatc caatgcccgc ggttacaccg
     2041 cctccagaat ctcggcaatc tcggctgtcc aagagaccac cgactgggca aaaagttcaa
     2101 tgggtgtcct gtcgccccga cggatatccg tcatttcttc aatacaccgt cggtgattgg
     2161 ggcctggcgg aggcagagat caacacggcc ctgcgcgtgc gattcacccg caattctggt
     2221 cgacatttcc agggtggtcc agtttccggc gtggtattct gagatgcgtg aatgacgtca
     2281 ctcaaatcct aaccgccatt gagcggggcg atccgcaggc gtccgagcaa ctcctgccac
     2341 tcgtctattc tgaattgcgc aaactggccg cacagcgact ggcccaggaa aagcctggcc
     2401 agacacttca ggcgacggct ttggtccacg acgcgtatgt ccggctggtg gacgccgacc
     2461 aactcgcgca ctggaacagc cggggtcact tctttgccgc ggctgcggag gccatgcgac
     2521 gaattctcgt cgaacaggct cggcgtaaac ggcgcatcaa gcacggcggt ggccgacggc
     2581 gcgtcgatct cgattcagcc atctccatga ccgagccgcc gtcacaggat ctgctggccc
     2641 tcgatgatgc tctgtcgaaa ctcgctgcaa tcgactccgc gaagtcggat ttagtgaaac
     2701 tacgcttttt tgcgggactg acgatgcctg aggctgcagc agccctcgga atctcgcttg
     2761 ctacggctga gcgccactgg accttcgccc gctcgtggc
//
LOCUS       DATMZQ010000528        13013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5110611, whole genome shotgun sequence.
ACCESSION   DATMZQ010000528 DATMZQ010000000
VERSION     DATMZQ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5110611"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1131
                     /locus_tag="VN496_16865"
     CDS             238..1131
                     /locus_tag="VN496_16865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXF18682.1"
                     /translation="MRLDQALARVMPEYSRNRLQEWMRAGYVTLDGGSASAKTKVWGG
                     EIVLIAPQTAASTAEHAAEAIPLAVMFEDDVMLVIDKPAGLVVHPGSGNWSGTLLNGL
                     LRHAPALAAVPRAGIVHRLDKETSGLMVIAKTIRAQVDLVRQLQARSVKREYIAVVHG
                     AVVRDGVVDAPIGRHPIHRTRMAVVARGKEAKTHYFPIVHGTHWSELRCRLESGRTHQ
                     IRVHMNSIGHPLIGDPVYGAKPQLVKALPVGARTFPRQALHAARLELIHPGSGKKLGW
                     DSALPADIATLISQLKNAEAA"
     gene            1115..1867
                     /gene="pgeF"
                     /locus_tag="VN496_16870"
     CDS             1115..1867
                     /gene="pgeF"
                     /locus_tag="VN496_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXF18683.1"
                     /translation="MLKQPKDWIIPDWPAPARVRAIITTRDGGCSIGSYASLNLGLQV
                     GDDEASVLTNRRIVKQFLPAEPKWLNQVHGTRVMPAESIAIPVEADASFASRPGCVCV
                     VLIADCLPILICERTGAIVAVAHAGWRGLVSGIIESTVRALQTQPQDLIAYLGPAIGP
                     SVFEVGSDVRDCFVAADSAAQYAFQRNNAGKWCADLFALARQRLVQLGVTDVYGGGLC
                     TYADSARFFSHRREKPTGRMAALIWIDDQSPA"
     gene            2045..3769
                     /gene="phaC"
                     /locus_tag="VN496_16875"
     CDS             2045..3769
                     /gene="phaC"
                     /locus_tag="VN496_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HXF18684.1"
                     /translation="MTSPAASPDQVLQDITSANAALLETFLRDLPSAGNTDLTALLRV
                     FTQADDAQLEKLRTLQMTLYRGHFTLWHQFAVPVNDLAFSAPTSDDRSEAPEWRLAFF
                     QYLQRAYQLNAQFLTEVGELAQLEPHAKRRLQFQLRQLTDAMSPKNSAATNPEVIKLA
                     AQTGGGSLVQGLKLLMADIARGRMSMTDEQAFEVGRNLAITPGAVVYENEVMQLIQYA
                     PATRDTYEYPLLIVPPFINKYYILDLQPENSFVRFCVEQGFTTFIVSWRNVTPQLGQL
                     SWSDYIEKGVLSPADVVKRISGATRINTLGFCVGGTLLACALAVMAARKENHAISLTL
                     LATMLDFSDTGDISVYIDQEYVERSEHEFRTSGCMPGSKLASAFATLRANDLIWHFVV
                     NSYLKGNLPRAFDLLYWNADGSNLPGRLYTYYLRNMYLENNLRIPGKLDACGVPLDLG
                     AIDIPAYILATKEDHIVPWRSAYASVGLLGGTSEFVLGASGHVAGIVNPPVKNQRHYW
                     LNSSLDAEPDAWLANATQNAGSWWNHWATWISSRSGALKPASAQLGSVQFPELEPAPG
                     RYVKEKSV"
     gene            3813..4556
                     /gene="phbB"
                     /locus_tag="VN496_16880"
     CDS             3813..4556
                     /gene="phbB"
                     /locus_tag="VN496_16880"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HXF18685.1"
                     /translation="MAKRIVLVTGGMGGLGESICTKMADQGYAVVTTYSPGNKTADDW
                     LAGMKQRGYDFRAYPTDVADFDSCVACVKQVQEKVGPVDVLVNNAGITRDMTFKKMTK
                     SDWDVVISTNLDSVFNMTKQVLDGMVERNWGRVINVSSVNAQKGAFGQTNYSAAKAGM
                     HGFTKALAYEVARKGVTVNTISPGYIGTKMVTAIPQEILDSKILPQIPLNRLGQPEEV
                     AGLIIYLASDEAAFVTGANISINGGQHMS"
     gene            complement(4740..5285)
                     /locus_tag="VN496_16885"
     CDS             complement(4740..5285)
                     /locus_tag="VN496_16885"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXF18686.1"
                     /translation="MVNPTTPNYAALNKIQLQSAVQTFMIAFGTWEMLLKLNVEATKS
                     LLQDAPASAQGLSAIRDAAGLSAWTSEQWRAGAEKLSGYSRNVYEIFGQTSGSLGNLL
                     EQTLLNTNTEIVELLDEVLKVSTGPQQEATAVAAKAAMANAKSVIEGISKAVRQTAGY
                     ADANVRAAAAATAEAVKNTAK"
     gene            5516..6130
                     /gene="phaR"
                     /locus_tag="VN496_16890"
     CDS             5516..6130
                     /gene="phaR"
                     /locus_tag="VN496_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HXF18687.1"
                     /translation="MTEEIRLIKKYPNRRLYDTATSSYITLADVKQLVLDQVIFRVVD
                     AKSEEDLTRAILLQIILEEESAGAPMFSSDMLSQIIRFYGNAMQGMMGSYLDKNIQTF
                     VEIQRRLQEQSIPKFGDNPMLNAEAWSQFVKMQGPTIQGLMSSYLEQSATAFLDMQQQ
                     LQKQARSIFGNFPFGTNPAGTAENKSDNSASDPDSFKQNKKTAS"
     gene            6197..7543
                     /gene="rimO"
                     /locus_tag="VN496_16895"
     CDS             6197..7543
                     /gene="rimO"
                     /locus_tag="VN496_16895"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXF18688.1"
                     /translation="MAITRSSSILKSAQPKVGFVSLGCPKATVDSERILTQLRAEGYA
                     ISGSYRDADLVVVNTCGFIDAAVEESLDAIGEALAENGKVIVTGCLGAKGDVVMQTHP
                     RVLAVTGPHATDQVMRAVHDHLPKPHDPFSDLVPPQGIKLTPGHFAYLKIAEGCNHRC
                     TFCIIPSLRGDLVSRPIGAVMQEAEHLVNAGVKELLVISQDTSAYGVDMKYRTGFWGG
                     RPLKTRITELAQALGELGVWIRLHYVYPYPHVDEMLPLMAQGKILPYLDVPFQHASPR
                     ILKLMKRPANAENNLERIRAWRNVCPDLTIRSTFIVGFPGETEAEFHTLLEFLKEAEL
                     DRVGCFAYSPVQGASANALPGALPVEVREERRARFMEVQAKISTRRLKRKVGQTLTVL
                     IDETANGRAVGRSTADAPEIDGSVHIRKPGHGKPGDFVKAHIYRADAHDLYGDVVA"
     gene            complement(7598..9544)
                     /locus_tag="VN496_16900"
     CDS             complement(7598..9544)
                     /locus_tag="VN496_16900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXF18689.1"
                     /translation="MVKSDLTHFFSDTGPLSGIIPAYRLRPQQLEMALAVERAIDNCS
                     VFIAEAGTGTGKTFAYLAPALTRGGKVIISTGTKTLQDQLFNRDIPTIRSAMKAPVTV
                     ALLKGRANYVCRYYLERAKNDGRFVNRDDIVYLARIESYAKTSKTGDRSGLGEVPEDA
                     TIWPQVTSTRENCLGGDCPHYKDCFVMEARKQAMEADVIVVNHHLFFADVVLRDEGVA
                     ELLPACNTVIFDEAHQLPETASLFFGETVSTSQLVELARDTRIEAATSAKDHAALPDA
                     AGALEKAARDLRLQFTEDTGRQPYSAVQSNPRFTRTLEDAITRLNGLTSILELQADRS
                     EGLRNCWTRAMDLLEGLTRWGEEEDRGYVRWIEIFGGSVRLHATPLSIAEIFHRQISG
                     HPRAWIFTSATLSVQGNFSHYQREMGLTGAANGFWESPFDYEHQALLYVPEGLPEPNH
                     EDFTRKAVEAALPVIEAAGGRTFFLFTSLRAMREARELIAVGMKKIGMNYPLLMQGEG
                     SRTELLERFRKLGNGVLLGSQSFWEGVDVRGEALSLVIIDRLPFSPPDDPVLAARIET
                     LNAEGRNAFMEYQVPQAVITLKQGAGRLIRDENDRGVLMICDPRLISKHYGKRIWRSL
                     PPMRRTRDVADVLSFYGEVSDAAA"
     gene            9724..10206
                     /locus_tag="VN496_16905"
     CDS             9724..10206
                     /locus_tag="VN496_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXF18690.1"
                     /translation="MLLIVLILIGAFAIVNWKAIIAPTALSFIVTDVQAPLGLILLSI
                     AGLLTVLFLLYVTYLQTAVLLDTRRHTRELQAQRELADQAEASRFTELRLFLETELHK
                     LANQSAEAQAKVTDRLEQVQRDLSAAMSQTETSLSAYIGELEDRMERTDESRKSGRSI
                     "
     gene            10302..10769
                     /locus_tag="VN496_16910"
     CDS             10302..10769
                     /locus_tag="VN496_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXF18691.1"
                     /translation="MLRIGIISDTHGLLRPQARSFLLGSDHIIHAGDICDPAILEELN
                     VLAPLTAVRGNNDKGAWAHNLPEVAVVKLDQVAICVIHDLNELDLDPGTAGIRVVVSG
                     HSHKPSAEERDGVLYFNPGSAGPRRFKLPISVGELIVAGNTVSERTVDLMRLG"
     gene            complement(10786..11634)
                     /locus_tag="VN496_16915"
     CDS             complement(10786..11634)
                     /locus_tag="VN496_16915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXF18692.1"
                     /translation="MDKAAAVIDQNRPSTVPDFKALKTRQQAAWASGDYALIGTTLQI
                     VGETLCEAVDLRAGQKVLDVAAGNGNASLAAARRWGNVVATDYVAALLDRARERAAAE
                     RLDIEFREADAEALSFADGSFDVVLSTFGVMFTPDQGRAAAELVRVCKHGGKIGLANW
                     TPEGFIGQLFKTIGKHVQPPAGAKSPALWGTQARLAELFGEHAKAIESSQRQFVFRYR
                     SPAHWMDVFRNFYGPVHKTFGALEPAAQTALQRDLLALIDQFNRAKDGTMVVPSEYLE
                     VVISRN"
     gene            11796..>13013
                     /locus_tag="VN496_16920"
     CDS             11796..>13013
                     /locus_tag="VN496_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXF18693.1"
                     /translation="MEIDGPDHAGRERSLTVRLFGPVTVLRNGIAVQLPASRKVRALF
                     AYLTLAPSPVRRTQLCDLLWDIPDDPRGELRWSLSKLRGVIDEPGKQRVYSNADAIGL
                     NLADCFVDALEIAGAAEQGIGTLSSGRQRMLSRLVCGDFLEGIEIDRNPLFNGWLIAQ
                     RRRFRGCHVALLEQLARNAGDEAAFEYLEQWLQLAPFDPHAHQLLLTALARHGRIEEG
                     ETHLKATVRQFEEEGLDGSAIRAAWRSARAQATSLHATFETVPAGSEIHERQMPVAAS
                     RRASIAVMPFADQSADTTVRGGAADALAHDVITRLAQLRSLFVIAQGTVFALHDRRIG
                     AEEAGRMLNVDYVVSGSVRRRGKQLTVSVELAQTRSARIVWAQLFNYEPTDAFVVLDE
                     IGNRIVASIASEIE"
BASE COUNT         2842 a         3635 c         3644 g         2892 t
ORIGIN      
        1 ccgatagcct tttggtagtt tttgctttcc agttcatcct tggcggcact gtaaatgcga
       61 tccggacccc aatccttggt ttcatcaaaa ttggtggcgc acccgaagag cagcaacagc
      121 aaaacaatcg ctaaactatc tttcatgtcc gaacccgtaa aaagtttgcc ggattatata
      181 gaaaacttcc cttgtgactc gcttcgtcgc gtcactgttc ccggcgattg tgccggcatg
      241 cggcttgacc aagcgctggc ccgggtgatg cccgaatact ctcgcaaccg actccaggaa
      301 tggatgcgag ccggatacgt tacgctggat gggggttctg cctcggcaaa aaccaaggta
      361 tggggaggtg agatcgtgtt gattgcgcca cagacagcgg cttcgaccgc ggagcacgcg
      421 gccgaggcga tcccactggc ggttatgttt gaggacgacg tcatgctcgt catcgacaag
      481 ccggccggtc tggtcgttca cccgggcagc ggcaattgga gcggcaccct gctgaacggc
      541 cttctgcgcc atgctccagc gcttgcggcg gtaccaagag ccggtatcgt acatcgcctt
      601 gataaagaaa cgagtgggtt aatggtcatc gcaaaaacta tccgcgccca agtggacctg
      661 gtgcgccagc tgcaagcgcg ttcggtgaag cgggaataca tcgccgttgt gcatggcgcg
      721 gtggtacgag atggcgttgt cgatgcaccg atcggccgcc atcccattca tcggacccgc
      781 atggcagtag tagcgcgcgg caaggaggct aaaactcatt attttccaat cgtgcatgga
      841 acacattggt cggaattacg ctgtcgcctg gaaagtggac gcacccacca gatccgcgtc
      901 cacatgaatt ccattggcca cccactgata ggtgatccgg tatacggagc caagccgcaa
      961 ctagtcaagg cgttgccggt tggcgcccgg acttttccgc gacaggcgtt gcatgcagca
     1021 cggcttgagc tgatccatcc gggcagcggc aaaaaactag ggtgggattc cgcactacct
     1081 gcggatattg ccacactgat ctcgcagctg aaaaatgctg aagcagccta aggactggat
     1141 tattccagat tggccagcgc ctgcgcgcgt gcgggcaatc attactactc gagatggcgg
     1201 atgcagcatc gggtcttacg ccagcctgaa tttgggactg caggtcgggg atgatgaagc
     1261 cagcgtgcta acgaaccgtc gcatcgtgaa acagttcttg cccgcggagc ctaaatggct
     1321 gaatcaggtt cacggcacgc gcgttatgcc cgctgaatcg atcgcgattc cggtggaggc
     1381 agatgcttcc tttgcatcgc gtccgggctg cgtctgtgtg gtgttgattg ccgattgtct
     1441 gcctatactg atttgtgagc gtaccggagc gatcgttgca gtggcccacg cgggctggcg
     1501 aggacttgtt tctggcatca tcgaaagcac ggtacgcgcg ctgcaaacgc agccacagga
     1561 cctgattgcc tacctgggtc cggctattgg accgtcagta ttcgaagtcg gcagtgatgt
     1621 gcgcgattgc tttgttgcag cagactcggc cgcacagtat gcgtttcaac gcaacaacgc
     1681 cggcaagtgg tgcgccgatc tgtttgcact ggccagacag cggctggtgc agcttggcgt
     1741 cacagacgtg tacggtggcg gactgtgcac atacgcggat tcagcgcgtt tcttttctca
     1801 tcggcgcgaa aagccgaccg gaagaatggc cgcgctgatc tggatcgatg atcaatcgcc
     1861 tgcttaatca gctgcgcggt atgtgattcc attagggttt agtattggcg tccttccatt
     1921 gcctttattg cgctggtgcg gcacttcgtg actggcatag catgattacg agcttgatcc
     1981 acggtaaccg gccatcagag gtttgcattt tttatagctt gcacgcgctt gtgcccatcg
     2041 cccgatgact tctccagccg cgtctccgga ccaggtattg caggacatca ccagcgccaa
     2101 tgccgccctg ctagaaacat ttctgcgcga tctgccatct gccgggaaca ctgatttgac
     2161 cgcgttattg cgtgtattta cgcaagcaga tgacgcccag cttgagaagc tgcgtactct
     2221 acaaatgacg ctttatcgcg gccacttcac gctatggcat caattcgctg ttccagtcaa
     2281 cgaccttgca ttttctgcgc cgacgagcga cgaccgttct gaagcgcccg aatggcggtt
     2341 ggcgtttttc caatatttgc agcgcgcgta tcagctcaat gcgcagttcc tcaccgaagt
     2401 gggcgagttg gcgcaactcg agccacacgc caagcggcga ctgcaattcc aattgcgcca
     2461 gcttacggat gctatgtccc ctaagaattc cgcagcgacc aaccccgagg tcatcaagct
     2521 ggccgcgcaa accggtggag gaagcctggt gcaaggtctg aagctgctga tggcagatat
     2581 cgcccgtggc cgcatgtcca tgactgatga gcaggctttc gaggtcggtc gtaaccttgc
     2641 aataacgccc ggcgcagtgg tctatgagaa cgaggttatg cagctgatcc aatatgctcc
     2701 tgcaacgcgc gatacttatg aatacccgtt gctgatcgtg ccgccgttta tcaataaata
     2761 ttacattctt gatttgcagc cggaaaattc cttcgttcgt ttctgcgtcg aacaaggttt
     2821 tacgacattc atcgtgtcgt ggcgaaatgt taccccgcaa ctggggcagt tgtcctggag
     2881 cgactacatt gaaaaaggtg tgctgtcccc ggccgatgtg gtgaaacgca tcagcggcgc
     2941 gaccagaata aacacccttg ggttctgtgt aggcggcact ttactggcgt gtgcgctggc
     3001 agttatggct gcgaggaagg aaaaccacgc catttcgctg accctgctgg cgaccatgct
     3061 ggatttctcc gacaccggtg atattagcgt ctatatcgac caggaatacg ttgaacgaag
     3121 cgagcatgaa ttccgcactt cggggtgcat gcctggcagc aagctcgcga gcgcttttgc
     3181 gacactgcgc gcaaacgacc tgatatggca tttcgtcgtg aacagttatc tcaagggtaa
     3241 tttgccgcgc gcgtttgact tgctttactg gaatgccgat ggaagcaacc tgccgggtcg
     3301 gctgtacacc tactacttgc gcaatatgta cctcgagaac aatctgagaa tccccggtaa
     3361 actcgatgcg tgcggtgtac ctttggatct cggcgcaatt gacatacctg cctatatcct
     3421 ggcgaccaaa gaagaccata tcgtcccgtg gaggtcggct tatgcaagcg tcggattgct
     3481 tggcggcacg tctgaatttg tgctcggtgc aagtggtcat gttgcgggaa tcgtgaatcc
     3541 gcccgtaaag aaccagcggc actattggct gaattcgtcc cttgacgccg aaccggacgc
     3601 atggcttgcc aatgcgaccc agaacgccgg aagctggtgg aaccactggg caacgtggat
     3661 aagctcccgc tccggggcac tcaaaccagc ctccgcgcag ctgggttccg ttcaattccc
     3721 ggaactggaa cctgcgccgg ggcggtatgt aaaagaaaaa tcggtttagc agtatcgacg
     3781 gaaacaaaaa gaacatatcg aggaggaagc atatggcaaa gagaatcgtt ctggtaaccg
     3841 gagggatggg aggtcttggg gaatccatct gcacgaaaat ggccgaccag ggctacgcgg
     3901 tcgtgacgac atattcgccg ggaaacaaga ccgcggacga ctggctcgcc ggaatgaagc
     3961 aacgcggtta cgactttcga gcttatccga cggatgtggc ggactttgac tcgtgtgtgg
     4021 cgtgtgtgaa acaggtgcaa gaaaaagtcg gcccggtaga cgtgctcgtc aacaatgccg
     4081 gcatcacgcg cgatatgaca ttcaagaaaa tgaccaagtc ggactgggac gtggttataa
     4141 gcaccaatct ggacagcgtc tttaatatga ccaagcaagt actcgacgga atggtcgagc
     4201 gcaattgggg gcgtgtcatc aacgtgtctt cggtaaacgc ccaaaaaggt gcatttggac
     4261 aaaccaatta ttcggctgcc aaggccggga tgcatggatt cacaaaagct ctcgcatacg
     4321 aagtcgcccg caagggcgtt accgttaaca ccatctctcc cggctatatc ggcacaaaga
     4381 tggtgacggc gattccgcaa gagattctgg actcgaagat cctgccgcag attccactta
     4441 accggcttgg ccagccggaa gaagtcgctg ggctcatcat ttatctggca tcagatgagg
     4501 cagctttcgt cacaggtgcc aatatttcca ttaacggtgg acagcacatg tcctgatgaa
     4561 agaaatgcgt ggccaggccg ccgtgacaac tgatatatga aattttgatt gcccttttaa
     4621 gtcatcggct gacgttttgc gcaccaattt gaggcaaaaa aaccccggtg ggttaccacc
     4681 ggggttcaac ctttaccaaa ggaggacctt tagataaagg gggaggagcc ttcaatccgt
     4741 tacttggccg tgtttttcac agcctccgct gtcgccgcgg ctgccgcgcg tacattggca
     4801 tccgcgtatc cggctgtctg tctgacagcc ttcgaaattc cttcaatcac cgacttcgca
     4861 ttcgccatgg ctgcttttgc agcgacggcc gttgcttcct gctggggtcc cgtagaaact
     4921 ttcagcactt cgtccagcaa ttccacaatc tccgtattag tgttaagcaa tgtctgttcc
     4981 agcaagttac ccaaacttcc ggaagtctga ccgaagattt cgtacacgtt cctggaatag
     5041 ccggatagtt tctcggcacc ggcgcgccat tgctcgctgg tccatgctga taacccggct
     5101 gcatctcgta ttgcggacaa cccttgagcg cttgcgggag catcctgcaa cagcgacttg
     5161 gtggcctcga cattgagctt caacagcatc tcccaagtgc cgaacgcgat cataaatgtc
     5221 tgcaccgcag actgaagttg tatcttgttc aatgccgcat aatttggggt agttggattt
     5281 accatgtaac ctcctagagc gaatgtagta ttggacacat catcgaacat acagacgttt
     5341 caattgatgc accgcaccaa gttattttag gatgtcgact ctttcctgtc aattttttct
     5401 gcggcaatgc ataaagttta gtaaaaccta attttcatgg ggttataatg cccgtctcgc
     5461 gcgctgccac agaacatgct gcggtctaat gggccgcttt ttccggactg cacaaatgac
     5521 tgaagaaatt cgtctgataa aaaagtatcc gaatcgccgt ttatacgata cggcaaccag
     5581 cagctacatc acgcttgctg acgtaaagca acttgtgctt gaccaagtta tattcagagt
     5641 cgtcgacgcg aagagtgaag aagacctcac ccgcgccatt cttctgcaga tcattctcga
     5701 ggaggaaagc gccggagcgc cgatgttctc atcggatatg ctttctcaga tcattcgctt
     5761 ctatggcaac gccatgcaag ggatgatggg cagctatctg gacaaaaaca tccagacgtt
     5821 cgttgaaatt cagcgcaggc tccaggaaca aagcatcccc aaattcgggg acaacccgat
     5881 gctgaatgcg gaagcatgga gtcagttcgt aaagatgcag gggccaacga tacagggctt
     5941 gatgtcgagt tatcttgaac agagcgcgac ggcctttctg gatatgcagc aacagttaca
     6001 aaagcaggct cgctcgatct tcggcaactt tcccttcgga acgaatccgg ccggcaccgc
     6061 ggaaaacaag tcagataatt ccgcgtccga tccagactca ttcaaacaga acaagaaaac
     6121 cgcgtcctga acaggacaaa ggattttggc gcggcaccga gttgagagct cggtgccgtt
     6181 tttcatcatt tgatttatgg ctatcaccag atcaagttcc attctgaaat ccgcacaacc
     6241 gaaagtcggt tttgtttcgc ttggatgccc taaagcgacg gtggactcgg aacgaatact
     6301 tacccaacta cgcgcagagg ggtacgcgat ttcaggcagc tatcgggatg cggatttggt
     6361 agttgtcaat acctgtggct tcatcgatgc ggcggtcgaa gaatcgcttg atgccatcgg
     6421 cgaggcactc gctgaaaatg gaaaagtcat cgtcaccggc tgtctgggag ccaagggcga
     6481 cgttgtaatg cagacgcatc ccagagttct cgccgtgact ggaccgcacg cgacagatca
     6541 ggtaatgcgt gccgtgcatg atcatctgcc caagccgcac gatccttttt ctgacctggt
     6601 gccacctcag ggaatcaagc tcacaccagg acactttgct tacttgaaga tcgccgaggg
     6661 ctgcaatcat cgctgtacgt tttgcattat tccttcgctg cgcggcgatt tggtgagccg
     6721 cccaattggc gcggtcatgc aggaagcgga gcacctggtg aacgcaggcg tcaaggaact
     6781 tctggtgatt tcccaggaca ccagcgctta tggcgtcgac atgaaatacc gcaccgggtt
     6841 ctggggcggc agaccgttga agacccgcat aacagaactg gcgcaggcgt tgggcgaact
     6901 gggcgtgtgg atacgattac actacgtcta tccttatccg cacgtcgacg aaatgctgcc
     6961 gctcatggcg caaggcaaaa tactgcctta tctggacgta ccatttcagc atgccagccc
     7021 gcgcatcctc aaactgatga aacgtcccgc aaatgccgaa aataacctcg agcgaattcg
     7081 agcatggcgc aacgtctgtc ctgatttgac tattcgcagt accttcattg tcggatttcc
     7141 aggcgaaaca gaagctgaat tccatacatt gctggagttc cttaaggagg cggaactgga
     7201 tcgcgttggc tgctttgcat attccccggt gcagggcgca tccgcgaacg ccttgccggg
     7261 agcactaccc gttgaagtgc gcgaagaacg tcgcgcccgc ttcatggagg tgcaggcaaa
     7321 gatcagtacg cggcgcttga agcgtaaagt tggccagaca ctgactgtct tgattgatga
     7381 aaccgccaac ggtcgcgccg tcgggcgctc aaccgcggac gcgcctgaaa tagacggcag
     7441 cgtacatatc cgcaaaccag gccacggcaa gcccggcgat tttgtgaagg cacacattta
     7501 ccgggcagac gcccatgatc tctacgggga tgttgtggcc tgatttgcgt cgacgcgttt
     7561 cggattcgat attgtcggcc gcgcgcagca aataaggtca ggcagctgcg tccgaaactt
     7621 ctccgtaaaa actcagcacg tcggccacat cacgggtacg gcgcatcggt ggcagactgc
     7681 gccagatacg cttgccatag tgtttggaaa tgaggcgcgg atcgcaaatc atcagcacgc
     7741 cgcgatcatt ctcgtcacgt atcaggcgac cggcaccctg cttcagcgta atgactgcct
     7801 gtggaacctg atattccatg aaggcattgc gtccttcagc gttcagcgtt tcgatgcgcg
     7861 ccgccagcac tggatcgtct ggcggtgaga acggcagtcg atcaatgatg actagcgaca
     7921 gggcctcgcc gcgaacgtcg acgccttccc agaaagactg gctgcccaac agcacgccat
     7981 tgccgagctt tcgaaagcgt tccagcagtt ccgtgcgcga tccttctccc tgcatgagca
     8041 aaggatagtt catgccaatt tttttcatgc ccactgcaat cagctccctg gcttcccgca
     8101 tagcccgcaa gctcgtgaac aagaaaaaag tgcggccccc ggctgcctcg atgaccggca
     8161 gcgccgcctc caccgctttg cgcgtaaaat cctcgtgatt tggttccggg agaccttccg
     8221 gcacgtataa caacgcttga tgctcgtagt cgaacgggct ctcccagaac ccgtttgcgg
     8281 cgcctgtcag tcccatttct ctttggtaat gggagaaatt gccctgcacc gacagtgtcg
     8341 cagacgtaaa aatccacgcc ctgggatgac cgctgatctg acgatgaaag atttcggcga
     8401 tagaaagcgg cgtggcatgc aagcgtacgg agcccccgaa tatttctatc caccgcacat
     8461 aacctctgtc ttcttcctca ccccagcggg tcagtccctc gagcaggtcc attgcacgcg
     8521 tccagcaatt gcgcaagccc tctgaccgat ccgcttgcaa ttccagaatc gacgtcaggc
     8581 cgttcaatct tgtaatcgcg tcctcaagtg tgcgcgtaaa ccgcgggttg ctttggacag
     8641 ccgagtaggg ttgccggccg gtatcttcgg tgaactgcaa acgcaggtca cgcgccgctt
     8701 tttccagcgc ccctgccgcg tcaggcaacg cggcatggtc tttcgcggac gtcgcagctt
     8761 ctatccgcgt gtcgcgcgcc agttcaacga gctggctggt cgatacggtc tcgccgaaaa
     8821 acaggcttgc ggtctctggc agctgatgtg cttcatcaaa aatcactgta ttgcatgccg
     8881 gcagaagttc ggcaacacct tcgtctcgca atacgacgtc ggcaaagaat agatgatggt
     8941 tcaccacgat aacgtctgct tccatcgcct gtttgcgagc ctccatcacg aaacaatcct
     9001 tgtaatgggg gcaatctccg ccaaggcagt tctcacgcgt tgacgttacc tgtggccaaa
     9061 tcgtagcgtc ttccggtact tcacccaatc cgctgcggtc gccggtcttg ctggttttgg
     9121 cataagactc tatgcgtgcc agatagacga tgtcgtcgcg attcacaaac cggccgtcgt
     9181 tttttgcccg ctccaaataa tagcggcaca cataatttgc gcgtcctttc agcaatgcaa
     9241 cggtgaccgg agccttcatg gcgctgcgga tggtcgggat gtcgcggttg aacaattgat
     9301 cctggagcgt cttggtgccc gtcgaaataa tgaccttgcc gcctcgcgta agcgcgggcg
     9361 ccagataagc gaaagtcttc cccgtgcccg ttccggcttc cgcaatgaat accgaacagt
     9421 tgtcgatcgc gcgttcgacc gcaagcgcca tttccagttg ctgcggacgc aaccggtatg
     9481 cgggaataat gcccgatagc gggccggtat cggagaagaa atgcgtcaga tcagatttca
     9541 ccatgtcatg attctagcgt gagccgatac ttcaatgttc atgctggcgc gtgctgccga
     9601 aagctgcatt tgcgcggctt tgcctttcta gaagtagaat cttgaaacta ctccgcttcc
     9661 gggcacgcca gattttcgtg atgcggatat caatggaggc tacttatgcg ggcccgtact
     9721 ctggtgttgc tgattgttct gatcctgatc ggcgcattcg cgatcgtgaa ctggaaggcg
     9781 atcatcgctc caaccgccct ttcatttatt gtcaccgacg tgcaggcccc gctcgggttg
     9841 atcctgctga gcattgccgg attgctcacg gttctgttct tgctctatgt aacgtaccta
     9901 cagacagccg tgctgcttga tacccgacgt catacgaggg aactacaggc tcagcgcgag
     9961 ctggcggatc aggccgaggc atcgcgcttt acggaacttc gccttttttt agaaacagag
    10021 ctgcacaagc tggcgaatca gtcggctgaa gcacaggcca aagtgaccga ccgccttgaa
    10081 caggttcagc gtgacctgtc cgccgccatg tcgcagaccg agacttccct ctcggcctat
    10141 attggagaac tggaggaccg catggaacgg accgatgaaa gtcggaagtc cggtcgttcc
    10201 atttgattcc agtgcaccgt gtgccgttga gcggcccgac gctcgggaca ggcttgagga
    10261 gatcgtgtgc agaacaatga aacgcggata gggcactcgc catgcttcgc attgggatca
    10321 tctcggatac gcatggcctg ctccggccgc aagccagatc ttttttgttg ggaagcgacc
    10381 acatcattca tgccggcgat atttgcgatc ccgcgatcct cgaagaacta aatgtcttgg
    10441 cgccgctgac cgcggtgcgc ggcaacaatg ataaaggcgc atgggcacac aacctacccg
    10501 aagtggcagt cgtcaaactt gaccaagtcg ccatctgcgt catccatgat ctcaatgaac
    10561 tggatctgga tccagggacg gcgggaatca gggtggtcgt atccggccat tctcataagc
    10621 cgtcagccga ggaacgcgat ggcgttcttt acttcaatcc cggcagtgcc gggccgcgac
    10681 gcttcaaatt gcccatctca gtgggcgaac tgatcgtcgc ggggaacacg gtctctgaac
    10741 ggacagtcga tcttatgcgc ctcggttgag tcgcacgacg atcgctcagt ttcttgaaat
    10801 gaccacctcc aggtattcgc taggcacgac catcgtcccg tctttcgccc gattgaactg
    10861 gtcgatcaac gctagaagat cccgctgaag cgccgtttgt gctgccggtt cgagcgcgcc
    10921 gaatgtcttg tgcactggcc catagaaatt ccggaacacg tccatccagt gcgccggcga
    10981 tcgatagcga aatacgaatt ggcgttggga tgactcgatt gcctttgcgt gttcgccgaa
    11041 cagctcggcg agccgggctt gggtgcccca aagcgctggc gatttggcgc cggctggtgg
    11101 ctgcacgtgc ttgccgattg tcttgaacag ctgcccgatg aagccctcgg gcgtccagtt
    11161 tgccaggcca atcttgccgc cgtgcttgca cacgcgtacc agttccgcgg ctgccctgcc
    11221 ctgatcgggt gtgaacatta cgccaaaggt cgataggaca acgtcgaaag agccgtcggc
    11281 aaacgatagt gcctccgcgt cagcttcgcg gaactcaatg tcgagacgct cagcagcagc
    11341 ccgttcacgg gcgcgatcca gcagcgcagc gacgtaatcg gtggcgacca cattgcccca
    11401 gcgacgcgca gcggcgagtg aagcgttgcc attgcccgcg gccacgtcta gcaccttttg
    11461 tcccgcacgc aggtcgaccg cttcgcacaa ggtctcgccg acgatctgca gagtggtacc
    11521 gatgagggca taatcgcccg atgcccacgc ggcctgctgg cgagtcttga gtgccttgaa
    11581 gtctgggacg gtggatgggc gattttgatc gatgacagca gcagctttat ccatgaaagc
    11641 tctcctttcg gttcgttggt cctgtgatgt ttgggatgcc tgcccggggg gccgcgatat
    11701 tgagacccgg ctccgaccag tgcggaacac agtctgtggc gttacgataa caacaagacg
    11761 tgggagccga cgtggtagcc ggcgtggaaa atgccatgga aattgatggt cccgaccatg
    11821 ctggccgaga acgctcgttg actgtgcgac tgttcgggcc tgtcacggtc ctgcggaacg
    11881 gaatcgccgt gcagcttccg gcatcgcgca aggtgcgcgc ccttttcgcc tacctcaccc
    11941 tcgctccgtc tccggtgcgc aggactcagc tttgcgatct attgtgggat atcccggacg
    12001 atcctcgcgg agaactccgg tggtcgctca gcaaacttag aggagtaatc gatgaacccg
    12061 gcaaacagcg tgtctactcg aatgcagatg cgatcggact gaaccttgcc gattgcttcg
    12121 tcgacgcact ggaaattgcc ggcgcggcag aacaggggat cggaacactt tcgtccgggc
    12181 ggcagcgaat gctgtccagg ctggtctgcg gagattttct ggagggcatc gagatcgacc
    12241 gcaacccgct tttcaacggc tggcttatcg cgcagcgacg gcgattcagg ggttgccatg
    12301 tcgcgctgct ggaacagctt gcccggaacg ccggcgatga agccgctttt gagtatctgg
    12361 agcagtggct gcaacttgca ccatttgatc cgcatgccca ccaattattg ttgacggcgc
    12421 ttgcgcggca tggccggatt gaagaaggcg agacacatct gaaagcgact gtccgccagt
    12481 tcgaggaaga aggtctggat ggttcggcaa tccgcgccgc ctggcgttca gccagagcgc
    12541 aagccacttc acttcacgcc acattcgaga ccgttccagc tggctcagaa attcacgagc
    12601 gtcaaatgcc cgtcgcagca tcgcggcgcg cgtcgatcgc agtcatgccc ttcgccgacc
    12661 agtctgcgga tacgacagtt cgcggcggcg cggcggacgc tctggcgcac gatgtgatta
    12721 cacggcttgc gcagttacga agtttgtttg tcattgccca aggcacggta ttcgcgctgc
    12781 atgacaggcg catcggcgcc gaagaggccg gacgcatgct taacgttgac tatgttgtca
    12841 gtggatctgt gcggcgccgt ggcaaacagc tcacggtgag cgtcgagctg gcgcagacgc
    12901 gatccgcccg gatcgtatgg gcgcagctgt tcaactacga acccaccgac gcctttgttg
    12961 tgctcgacga gatcggcaac cgaatcgttg catccatcgc cagcgaaatc gaa
//
LOCUS       DATMZQ010000529         4276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1645497, whole genome shotgun sequence.
ACCESSION   DATMZQ010000529 DATMZQ010000000
VERSION     DATMZQ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645497"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /gene="aroB"
                     /locus_tag="VN496_16925"
     CDS             <1..576
                     /gene="aroB"
                     /locus_tag="VN496_16925"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549475.1"
                     /note="catalyzes the formation of 3-dehydroquinate from
                     3-deoxy-arabino-heptulonate 7-phosphate; functions in
                     aromatic amino acid biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXF18694.1"
                     /translation="IAEAVKVSLIRDSLFFEWLEGNADALRECAPPAMARMIRRCAEL
                     HMRQIAHGGDPFETGSARPLDYGHWSAHKLESLTAHELRHGEAVAIGLALDARYSVQI
                     GMLAAHGEERVYGLLKRLGFYLWHPAMDTRDEHGRPVILRGIEEFREHLGGELTITLL
                     REIGRGEEVHAMDSNEILHAMAWLRRKETGQ"
     gene            573..1766
                     /gene="eboE"
                     /locus_tag="VN496_16930"
     CDS             573..1766
                     /gene="eboE"
                     /locus_tag="VN496_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HXF18695.1"
                     /translation="MRIGANGAHLTYCSNIHPGESWAEVRANIERYFPAVRDRVAPGE
                     MFGIGLRLSARAARELNSGNALPEFREFLTRNRLYVFTINGFPYGTFHGARVKEEVYL
                     PDWRDDERLRYTDELAELLLRLLPDGVEGSISTVPGAFKPAMRPVGDVEKIADNMLRH
                     VATLVDIERRSGRHIALAIEPEPHCFLETIAESVQFFRQHLFSDGAARRLAELARMDA
                     QTADAALHAHVGLCLDLCHAAVEFEDPAGCIRTLQEAGIRVSKLQISAGLRLPQLTAD
                     AVKALRQFDDPVYLHQVVETGPHGVRRYADLPEALATTTGTADGREWRVHCHVPIFMD
                     DLGAFASTRSFIRDVLEIHRLHPVSGHLEVETYTWNVLPQRLRAGGMEQAIARELNWV
                     KEALA"
     gene            1763..2635
                     /locus_tag="VN496_16935"
     CDS             1763..2635
                     /locus_tag="VN496_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXF18696.1"
                     /translation="MNFGVALKLGRVSNLPTVWSNVLTGMALAGTAMVDYRLLLLVVS
                     LSLFYVGGMFLNDAFDHGFDAKARPERPIPSGQVSAVQVHAFGFGMMAVALALLGWIG
                     YGDEPFTQWRPVLAGFALASAIVFYNWHHKNNPLSPLVMGVCRMLVYLTAALAVTEVV
                     PSQVIVAALVLLSYLIGLTYAAKKEHLGRLENVWPLGFLAVPVAYGIYLAPVQPLVVL
                     PLVSLAAWASYALWLLRRRKPGDVPRAVVSLIAGISLLDAMLLAGTGHVLFAWLAVGS
                     FVITLLLQRWITGT"
     gene            2750..3103
                     /locus_tag="VN496_16940"
     CDS             2750..3103
                     /locus_tag="VN496_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXF18697.1"
                     /translation="MKNRYLAAVLAGLCGFPAASGLAEADTVRGNELFQQYCQGCHGP
                     DGRGGRKDGFMPRPQNLTKKGYIDQLPDEYLFTVITKGGEGVNKSGYMPSFGTIFSEA
                     DIGNLIAFIRSLARY"
     gene            3133..>4276
                     /locus_tag="VN496_16945"
     CDS             3133..>4276
                     /locus_tag="VN496_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18698.1"
                     /translation="MRKPLASVILLTGYLFCISAMAGDLTQWIKQTPVPPNPSKVVVP
                     PGYKVGILVAGLSTPSSATVDGQGNIWVAISGNLFGGPDADLMPKPHVEVYSPSGKLI
                     KKIGEGLFKTVMNEIAYCPENGVTYIPEYGERIWEMKGLDGEPKVIIKDLHNGDHRNA
                     GITCRGGYLYFALGQPSNEGFADPDNLGWTDIPNDPFWLAHPDGIGNTPHDAPCRDIV
                     HTGLNVKTSDGRMTGAYMPAGVAAKPGQIVKATVPCHGSIMRVKFADKNAEGIYPHDK
                     MEVYAMGFRNQSGVEFGPKGSRFESALAVSDNGMNDLGNRRIANGAEKIWLVTEKGQD
                     AGFPDKEGMNFVTNKRFGWKAYTGSEVDRPHPQLYIGDKPFVPKNSP"
BASE COUNT          852 a         1213 c         1348 g          863 t
ORIGIN      
        1 attgccgaag cggtgaaggt ctcactgatt cgcgacagcc tgtttttcga atggctggaa
       61 ggcaacgcag acgcattgcg cgaatgcgcg ccgccagcca tggcccgcat gatccgccgt
      121 tgtgccgagt tgcacatgcg gcagattgcc catgggggcg atccgtttga gacgggcagt
      181 gcacggccgc tggactatgg ccactggtcg gcgcacaaac tcgaaagcct gactgcgcat
      241 gaactgcggc atggcgaagc agttgcaatc ggcctggcac tggatgcacg ttattccgtg
      301 cagatcggca tgctggctgc gcacggcgag gagcgcgtgt acggcctgct caagagactg
      361 ggtttttatc tgtggcatcc ggccatggat acacgtgacg aacacggacg cccggtaatc
      421 ctgcgcggca tcgaggaatt tcgcgagcat cttggcggcg aattgaccat cacccttctg
      481 cgtgaaatcg ggcggggcga agaagtgcat gccatggaca gcaacgaaat cctgcatgcg
      541 atggcatggc tgcgccggaa agaaaccggt cagtgagaat tggcgcgaac ggcgctcacc
      601 tgacgtactg cagcaacatc caccccggcg aatcctgggc ggaagtgcgg gccaatatcg
      661 aacgttactt tccagcagtg cgcgatcgcg tggcgccggg cgaaatgttc ggcattggtt
      721 tgcgactgtc cgcacgcgca gcgcgcgagt tgaacagcgg taatgcattg ccggaatttc
      781 gcgaattcct gacgcgcaac cggctctatg tgttcaccat caacggtttc ccatacggga
      841 ctttccatgg cgcgcgcgtc aaggaagagg tctatcttcc cgactggcgc gacgacgaac
      901 gattgcgcta taccgatgag ctggcggaac tactgctacg gctgctgccg gatggtgtcg
      961 agggcagcat cagcaccgtg cccggggcat tcaagcccgc gatgcgcccc gtcggcgacg
     1021 tggaaaaaat cgccgacaac atgctgcggc acgtggccac gctggtggac atcgagcgcc
     1081 gcagcggccg ccatattgcg ctggcgatcg agccggagcc gcattgtttc cttgagacga
     1141 ttgccgagag tgtgcagttt ttccgccagc atctgttcag tgacggcgcg gcgcgacggc
     1201 tggctgaact ggcgcggatg gatgcgcaga ctgcggacgc ggcgctgcat gcgcatgtcg
     1261 gcttgtgcct tgatctgtgc catgcggcag tcgaattcga agacccggcc ggttgtatcc
     1321 gcacgttgca ggaggccggc atccgtgtga gcaagctgca gatcagtgcc gggctgcggc
     1381 taccgcaatt gactgccgat gcggtcaagg cgctgaggca gttcgatgat ccggtgtacc
     1441 tgcatcaggt tgtggaaaca ggtccgcacg gcgtgcgccg ctacgcagac cttcccgaag
     1501 cgctggctac aacgaccggc acagcggatg gacgcgaatg gcgggttcac tgccatgtgc
     1561 ctatcttcat ggatgacctg ggcgcgtttg cttccacccg ctccttcatt cgggacgtgc
     1621 tggagatcca ccggctgcat ccggtgtccg gacatctgga agtggagacc tatacctgga
     1681 atgtgctgcc gcaacggctg cgcgccggtg gcatggaaca ggccattgcg cgtgaactga
     1741 actgggtaaa agaggcgctg gcgtgaattt cggcgtcgcg ctgaagttgg ggcgcgtgtc
     1801 aaatctgccg acggtctggt ccaacgttct caccggcatg gcgttggcgg gtaccgcaat
     1861 ggtggattac cgacttctgc ttctcgttgt ctcgctgtcg ttgttctacg tcggcggcat
     1921 gttcctcaac gacgcgttcg atcacggctt cgatgcaaag gcgcggccgg aacggcctat
     1981 tccttcaggg caggtgagcg ccgtccaggt gcatgcattc ggcttcggca tgatggctgt
     2041 ggcgcttgcg ctgctgggct ggatcgggta cggcgatgag ccgtttaccc agtggcgtcc
     2101 cgtcctggcg ggatttgctc ttgcgtcggc gatcgtgttc tacaactggc atcacaagaa
     2161 caatccgctg agcccgctgg tcatgggcgt gtgccggatg ctggtctatc tgaccgcggc
     2221 gttggctgtc accgaggtcg tcccgtcgca agtgatcgtg gccgcactgg tcctgctcag
     2281 ctacctgatt ggattgacgt acgctgccaa gaaggaacat ctcggtcgtc tggaaaacgt
     2341 ctggccgctg ggcttcctcg ccgtgccggt agcgtatgga atttatctcg cgccggtgca
     2401 gccgctggtc gtattgccgc tcgtctcgct cgccgcgtgg gcgtcctacg cgctgtggct
     2461 tttgcgcagg cgcaagccag gcgacgtacc gcgtgccgtg gttagcctga tcgcgggcat
     2521 ttcgctgctc gacgcgatgt tgctcgccgg aaccgggcat gtgctctttg cgtggctggc
     2581 ggtcgggtca ttcgtcatta ctctcttgct ccaacggtgg atcaccggca cttgagcgtc
     2641 cagggctgtg tttcgcaatc cagttcgcga tttgttggct cagcgggtgt aggcttccgg
     2701 gattgccggt gtgaaaaaaa agagcagttg caagcgggag actccctgga tgaagaaccg
     2761 atatttagcg gccgtattgg ccgggctgtg tgggttcccc gcggcgtctg gattggccga
     2821 agcagataca gttcgtggca acgaactgtt ccagcagtac tgccagggtt gccacgggcc
     2881 ggacggccgt ggcgggcgca aggatggttt catgccgcgt ccgcagaatc tgaccaagaa
     2941 gggctacatc gaccagctac ccgacgaata tttatttacc gtgataacga aaggaggaga
     3001 gggcgtaaac aagagcggct acatgccgtc gttcggaacg atattttccg aagccgacat
     3061 aggtaatctg atcgcattca ttcgaagtct ggcgcgatac taaaaaaacg actattcagg
     3121 aggagagcca agatgagaaa accactagca agcgtgattt tgttaacggg atatctgttc
     3181 tgcatttccg ctatggccgg cgatctgacg caatggatca agcagacgcc ggtgccaccc
     3241 aatccatcca aggtcgtcgt gccgcccgga tacaaggtcg gcatcctcgt cgccggactc
     3301 agtactccct cgtctgccac ggtggacggg caggggaaca tatgggtggc aatctctggc
     3361 aacctgttcg gcggccctga tgccgatctc atgccaaagc cgcacgtaga ggtttacagc
     3421 ccaagcggaa agctgatcaa gaaaatcggc gaaggcctgt tcaagacagt catgaacgaa
     3481 atcgcgtatt gccccgagaa cggcgttacg tatattccgg aatacggtga gcgcatctgg
     3541 gaaatgaaag gactcgacgg cgagcccaaa gtcattatca aggatctaca caacggcgac
     3601 catcgcaacg cgggcattac ctgccgcggc ggctatctgt atttcgcact cggtcagccc
     3661 agcaacgagg gctttgcgga tccggacaat ctcggttgga ccgatattcc gaacgacccg
     3721 ttctggctgg cgcaccccga cggcatcggc aacaccccgc atgacgcacc ctgccgcgac
     3781 attgtccata cggggctcaa cgtaaagacg tccgacggac gcatgacggg cgcctacatg
     3841 ccggccggtg tcgccgcgaa accgggccag atcgtcaagg cgaccgtacc ttgccacggt
     3901 tccatcatgc gtgtgaagtt tgcggacaag aacgccgaag gtatctatcc gcacgacaag
     3961 atggaagtct atgcgatggg gtttcgcaat cagtccggtg tggaattcgg tcccaaggga
     4021 agccgtttcg aaagcgcgct cgccgtatcc gacaacggca tgaatgacct gggcaatcgc
     4081 cgtatcgcaa acggggcgga aaagatctgg ctggtcacgg aaaagggcca ggatgcagga
     4141 tttcccgata aggaaggcat gaacttcgtc accaacaagc gcttcggctg gaaggcctac
     4201 accggctcgg aggtcgatcg cccgcacccg cagctttata tcggcgacaa acctttcgta
     4261 cccaagaact cgcctt
//
LOCUS       DATMZQ010000530        13795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5115591, whole genome shotgun sequence.
ACCESSION   DATMZQ010000530 DATMZQ010000000
VERSION     DATMZQ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5115591"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VN496_16950"
     CDS             <1..339
                     /locus_tag="VN496_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HXF18699.1"
                     /translation="KVAQWVRLPAHCFAHALVSEYRPGTPLGWHRDVPNFELIVGVSL
                     RGACRMRLRPYRPGERNRREDVIVLELQPRSAYQIRDTARWSWQHSIVATAQQRYSIT
                     FRTARRQLKK"
     gene            complement(353..553)
                     /locus_tag="VN496_16955"
     CDS             complement(353..553)
                     /locus_tag="VN496_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18700.1"
                     /translation="MRPLLIIFPGETRKWRVELANGDHAVDHASTDTARLDVMYWTQV
                     NTPADALLYSENGDLEKLNHTK"
     gene            856..1008
                     /locus_tag="VN496_16960"
     CDS             856..1008
                     /locus_tag="VN496_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18701.1"
                     /translation="MLGTRIPGFGIGPQRFRQFIPTHRGGIKKLLQKIQGSGVDAQPF
                     LPGRGF"
     gene            1033..1269
                     /locus_tag="VN496_16965"
     CDS             1033..1269
                     /locus_tag="VN496_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18702.1"
                     /translation="MTKILRFLDSSGDREIMFDDSEAMAEARAEAQRLFESSLAAGAV
                     AFGVNRTGGQPDRKVGDFSSLEDETVVVPRIVGG"
     gene            1360..1803
                     /locus_tag="VN496_16970"
     CDS             1360..1803
                     /locus_tag="VN496_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18703.1"
                     /translation="MEIRFNRILTALPHLRRSVRRLFFDEEDAMEIRVLFMDAPGSHS
                     AAEARATQLLIRVLDPMQRQEYLRYGCFTVEAPGWGRFQILSRSVFNVLSMETGLAYC
                     AAPNIPVPIPDLMLSQKLLLEHDPGRFFQVANQRRQDYSRSNVSL"
     gene            complement(1841..3025)
                     /locus_tag="VN496_16975"
     CDS             complement(1841..3025)
                     /locus_tag="VN496_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF18704.1"
                     /translation="MAEAKVQFWRVIPALGIAQIISWGTLYYSIAVLSSPIQATLGIS
                     QTMLFGAFTIGMLISGAASPTVGKLVDERGGRTVLTAGSLLGAAAFLTLSLAKDPVVF
                     LSGWLLGGVAMAATLYEPAFATLHQLSREHYRRSVTALTLIAGFASTSFWPLTTVLEA
                     GIGWRGAFAVFALLHLLVCVPLHWFVVPNAKTPVHPKNIVSSPSTERDRQSTSYFWLA
                     TAFSIAMFVMAVIFAHLIEMLKARGISSRDAVLVGSIIGPMQVAARIVEFNFARHARA
                     TRVGMIAFGMFAVAILVLAFAGHSFGWAVAFAILFGASNGIMTIVRGTAPAELFGQQR
                     LGSLLGRLARPAFIAKAIAPVMFAALIARGLPAGWAEAGLGLVAFLGFISFVIAVRSA
                     KA"
     gene            complement(3123..3734)
                     /locus_tag="VN496_16980"
     CDS             complement(3123..3734)
                     /locus_tag="VN496_16980"
                     /inference="COORDINATES: protein motif:HMM:NF025731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HXF18705.1"
                     /translation="MTEAVSKYFPGMEQWLIVLAPLLLIAYFIPLFIAAGRRHRFIPA
                     IGLINLALGWTVLGWLGAIIWAVNRDVRGRDVEPQPAPLDFMNEPRLIEPGENSQAIE
                     VGSSKKCLYCAESIRAEALVCRYCGHDVGAPGAATPAVAKSTTIDQSIEELQALLKDH
                     EQSVEERFPVSEPATNYVPPQEAVTAPIPVEVARQLSGWKKAG"
     gene            4008..4988
                     /locus_tag="VN496_16985"
     CDS             4008..4988
                     /locus_tag="VN496_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXF18706.1"
                     /translation="MASNSSALQSQNIVPWQPSQRYPDPAIQILDQSFARYRLNSASV
                     ERIAHGFRWCEGPVWFGDGRYLLWSDIPNNRMLKWEEETGIVSIFRKPSGFANGNTRD
                     RQGRLITCEHGGRRVSRTEYDGTITPIADRFESKPLNSPNDVVVKSDGSIWFTDPPFG
                     LLGNYEGHTAKQQLPMNVYRVDGTSGEITVVEGEVNRPNGLCFSPDESILYLVESGST
                     PRNIYAYDLTDDGKRLTRKRKFLDAGAGTPDGMRCDVDGNIWFGWGMGDAELDGVMIH
                     NADGRLIGRITLPERCANLCFGGSARNRLFMAASKSVFALYVNTQGVAGG"
     gene            complement(5004..5432)
                     /locus_tag="VN496_16990"
     CDS             complement(5004..5432)
                     /locus_tag="VN496_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF18707.1"
                     /translation="MPNSENKPVQRGSEPTEPSVRRRILVVDDNEDAASTLAMLLQLT
                     GNEVHTACDGLEAVKAVADLQPDVVLMDIGMPKLNGYDAARRIRGHASGKDTFLIALS
                     GWGQEEDKVRSANAGFNAHITKPVDVSTLNKLLADHAVRP"
     gene            complement(5909..7204)
                     /locus_tag="VN496_16995"
     CDS             complement(5909..7204)
                     /locus_tag="VN496_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXF18708.1"
                     /translation="MDERFTPEFRRRTIVKVSWRLLPLVVVSYLVAYIDRTNVAFAAL
                     TMNKDLGLSAYAYGWGAGIFFLGYALFEVPSNIVLEKVGARRWIARIMFTWGIVSGLT
                     AVATGSASFIFLRFLLGVAEAGFFPGMILYFTYWFPARYRGRVISTLFIAVPLGNALA
                     SVVSGAVLGMDGLLGLKGWQWVYIVEAIPAVLLALVVWVQMTDRPAVAKWLKPDEKEW
                     LETELKLERANIESAGSLTLLRALTDRRVLALSMIYFTSVVAGYGITFFLPQIVKGLG
                     HSNFITGLLTAIPYVVGVVGLIFWGHSSDRHSERRWHLICASTLAAIGLAGTAFFTGS
                     AWALVTMSVAAIGLYGSRPSFWPMPSLFLTGAAAAGGIALINSIGNLGGYVGPFVVGW
                     IKDSTKSFEMGVYFLAACAFTSALITYFATRNHLLDARK"
     gene            7460..7906
                     /locus_tag="VN496_17000"
     CDS             7460..7906
                     /locus_tag="VN496_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXF18709.1"
                     /translation="MGTLNVNDRQVNVNVENEMPLLWVLRDELGMTGTKYGCGIAMCG
                     ACTVHIDGKPVRSCVMPISAVTGKVTTIEGLSRDGAHPLQKAWVKLQVPQCGYCQSGM
                     IMAAAALLKENKHPSDAQIDAAMTNLCRCGTYVRVREAIHLVAQSA"
     gene            7918..10074
                     /locus_tag="VN496_17005"
     CDS             7918..10074
                     /locus_tag="VN496_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXF18710.1"
                     /translation="MKAQTFNAGRRDFLVKSAAVSGGLTLGIHLSGNVLAADAPAITE
                     VTHWIVIQPNDVVLIRIARSELGQGSFTGLAQLVAEELECDWSKVRSEYADVNDHVRR
                     NRIFGSMSTGGSRSIRESQEYLRKAGASAREMLVAAAAQEWGVPAAECKAARSVISHP
                     SGKSTTFGKVAAAASKLEVPKEPKLKDPKDWKLIGTSPPRFDIADKTTGKQIYAADVR
                     LPGLLHASIVQCPVFGGKLKSYDESAIRDVIGVKRVVTGDDWVAVVANNWWRANQALK
                     ELPVEWDVGESGKVSSDSIMQFLRTGLDATDVPVARKDGDAQAALGNAVKVIEAEYHA
                     PYLNHATMEPQTATAIVTDDKVEVWVGTQNGESTIAAASEAAGIPLENVIVHKMHAGG
                     GFGRRGPHQEYTKQAIWIAQKMPGTPVRLQWSREEDMKQGRYRPVALVKLRAGLDKDG
                     NWSAWHVRQADQSIFITVRPADIKNGIDPVNVRSFQDNPYAVPNFIDEYAMRNTHVPP
                     GFWRAVAHTHNPFFRECFIDEVVHAAGKDPVEFRRPLLQGKKDLGVLEAVVKAASWDK
                     PPAKGIYRGIAVVDSYGSFSAAVVEIAVTNASVIEVKRVVVALDSGYVVHPDAVLAQV
                     QSGVIWGLSSAMHEEITINEGRVVQSNFSDYRVLTLAETPKIECVLVPTGGFWGGVGE
                     PPIGAVIPALGNAIFAATGKRVRSLPFKNHGFSYKT"
     gene            10081..10620
                     /locus_tag="VN496_17010"
     CDS             10081..10620
                     /locus_tag="VN496_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HXF18711.1"
                     /translation="MRECREYVNVVRYMKLLVFATDDIHDSHDRHGIFASRRRALRAL
                     AAGACLLTIKPVMATPDELADTLRQTFGDRAINRGKVKLEMPRLAENGSVVPITVSVD
                     SAMTPQDYVKSIHVFASENHLPRVLEVQLGPHNGKGVFSSRVRVARSQPITAVALMSD
                     DTVWAAAVNVEVVTSECGL"
     gene            10626..10946
                     /gene="soxZ"
                     /locus_tag="VN496_17015"
     CDS             10626..10946
                     /gene="soxZ"
                     /locus_tag="VN496_17015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HXF18712.1"
                     /translation="MGTARVRMPESAKRGEVFEIRTMVQHPMESGFRLDNRGKEIPRH
                     IVESFVCNYNGKEIFRATLHPAVSTNPFFTFYVVAAESGELSFTWQDDQGGVATATQR
                     LTVS"
     gene            complement(11041..12540)
                     /locus_tag="VN496_17020"
     CDS             complement(11041..12540)
                     /locus_tag="VN496_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXF18713.1"
                     /translation="MPKSKLNSSDRDFFSALGNVVFGNPFSAQRDELIVRLAPGAKAG
                     DLTNDREALARVVGPRLGPWLREGMPAVNELSADDRKLLEPAVLYVCYHRYVPQLDNL
                     IERQAMQGGASLAVPFAGDAIAQLVGGGLEEERAVRYFAFYFQLRRAFYFIHRGLAGE
                     CPSMQRLREALWNNIFTYDMRGYEATLWNRMEDFSTLLLGETGTGKGAAAAAIGRSGF
                     IPYLPEQRRFAANFTETFISINLSQFPEALIESELFGHRKGAFTGAIDHHDGVFARCS
                     AHGALFLDEIGEVSIPVQIKLLQVLQERTFTPLGGHEKKRFAGRAIAATNQSLGKLRR
                     EGRFRDDFFYRLCSDVIHVPTLRQRIAESPAELKHLVQLLVARITGSEESDIVVTVLG
                     ALTKNLPEGYAWPGNVRELEQAVRRILLTGAYTADPLPLLGQEETLLERLRSGDMTAA
                     ELLARYSALLYRRFGTYSEVAKRTGLDPRTSRKYVENSGAEKVESEVEK"
     gene            12729..13562
                     /locus_tag="VN496_17025"
     CDS             12729..13562
                     /locus_tag="VN496_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18714.1"
                     /translation="MKTITKQSVTALLSIAMFAGCATPNGTDGQVAGTTFAQGVKNLV
                     LSPFMIVAGIAQGLAFLPYTIGTGIGELNKGLLQANAVPLDDSYKATFGVSIDDQRVD
                     QQTGKIQGQPGQYGRYRSQAIFEANSALQRLLVSQGMPEAKAKNYVLVGNYQYAFTRG
                     QILLAVVYRHPGEQPFRVSAKQTQIATTFRPGQRGWHEPYQFDSEGHPLDEVIDWAAI
                     DYKLLRQEKIVATLMVLAAEAIKSGKRSPEYWDVEKPWMAGDTTKVMATSQGRVSLPS
                     A"
     gene            13613..>13795
                     /locus_tag="VN496_17030"
     CDS             13613..>13795
                     /locus_tag="VN496_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18715.1"
                     /translation="MKFKLKIITGLATLALLHGCGSGDEFGEVTVHTQPGPPQETPYA
                     PCGTYNGQPVVCQPLRP"
BASE COUNT         3038 a         3921 c         4102 g         2734 t
ORIGIN      
        1 aaggttgcgc agtgggtgcg cttgccggct cattgctttg cgcatgcgct cgtaagcgaa
       61 tatcggccgg gaacgccttt gggctggcat cgcgatgtac caaacttcga actgatcgtc
      121 ggagtatcac tccgcggcgc atgccgcatg cgcctcaggc catatcgtcc gggtgagcgc
      181 aaccgcaggg aggacgtcat tgttttggag ttgcaaccgc gatccgctta ccagatccgc
      241 gataccgcgc gctggtcatg gcagcacagc attgtcgcca ccgcacaaca gcgctattcg
      301 atcacatttc gcactgcacg ccggcaattg aagaaataac gagccgttcg ggctacttcg
      361 tgtggttcag cttctcgaga tcgccgttct cgctgtacag caaggcatcg gccggcgtat
      421 tgacctgtgt ccagtacatc acatcgagac gcgcagtgtc tgtactggcg tgatcaaccg
      481 catggtcgcc attggccaat tccacccgcc actttctggt ttcccccggg aagatgatta
      541 acaggggccg catataacct ctatcgctgc tttatcggat tgaccatatt cgtagacttt
      601 tctactggat acatgttccc gatacggcgc gctttgcgtt ttcggaaacc gcgccatcat
      661 tagtaattct ttcagatgga acgtgacgct gaaaccattt ttaagaacgc tgggtcgaag
      721 cttacgggta caggttcttt tgttcgaagc aggtttctgc aggcgcttcg gcttcccctg
      781 tgccctggat caaaaagcac ggcaacgggc cgcgcggtgg caagaggagc cgcgtggtca
      841 aaaaggagtt caatcgtgtt aggcacccgt attcctggat ttggcatcgg gccgcagcgt
      901 tttcgccagt tcattccgac ccatcgcggc gggataaaaa agctattgca aaaaatacag
      961 ggcagcgggg tcgacgcgca gccgtttctg ccaggaagag gtttttgata ctggctaaag
     1021 taaggagaca tcatgaccaa gatactgcga tttctcgatt cttccggcga ccgggagatc
     1081 atgtttgatg acagcgaagc aatggcggaa gctcgtgccg aggcccagcg tctgttcgag
     1141 tcgagccttg cggccggagc ggtcgcgttc ggcgtaaatc ggacgggcgg acagcctgat
     1201 cgcaaagttg gcgatttctc ttcgctggaa gacgagacgg tcgttgttcc ccgcatcgtc
     1261 ggaggctgaa ggaaaagcgg ctgttgcagg ccggtaacgc aggtcggacg ctcgccctgc
     1321 ccgcgcggcg gggcgagtct tcggaattct cgcgaggtaa tggaaattcg cttcaatcga
     1381 attttgacgg cgttaccgca tttgcgccgc agcgtcaggc gcctgttctt cgatgaggag
     1441 gacgccatgg agataagggt actgtttatg gatgccccag gatcgcattc ggccgcggaa
     1501 gcgcgtgcca cacagttgct gattcgggtt ctcgacccga tgcaacgtca ggaatatctg
     1561 cgttacggct gctttaccgt cgaggcgccg ggctggggtc gttttcagat cctgtcgaga
     1621 agcgtattca acgtactgag catggagacc gggctggcgt attgcgccgc tccgaatatc
     1681 ccggtgccga ttcccgacct catgctctcg cagaagctgt tgctggagca cgacccgggg
     1741 cgtttttttc aggtcgcgaa tcagcggcgg caggattaca gcaggtccaa cgtcagcttg
     1801 tagcgggtag cagggaagat cgctggcaat caacccactg tcaggccttt gccgagcgca
     1861 ccgcaatcac aaagctgata aagccgagaa acgcaaccaa ccccaagccg gcttccgccc
     1921 agccggcggg caggccacgg gcaatcaaag cggcaaacat caccggcgcg atggcctttg
     1981 caataaacgc aggccgggcc agccggccca gcaatgaacc caaccgctgc tgaccgaaca
     2041 actctgcagg cgctgtgccg cgcacgatgg tcatgatgcc attgctcgcg ccaaacagaa
     2101 tcgcaaaagc gaccgcccaa ccgaagctat ggcccgcaaa tgccaggacc aggatcgcga
     2161 cggcaaacat cccgaaggcg atcatgccga cccgtgttgc gcgcgcgtgg cgagcgaagt
     2221 tgaattcgac gatgcgggcg gcaacctgca tgggaccgat gatcgacccg accagtacgg
     2281 cgtcccgcga agatatgccg cgcgctttca gcatttcgat cagatgggca aatatcaccg
     2341 ccatgacaaa catcgcaatc gagaacgcag tggccagcca gaaatagctt gtcgattgcc
     2401 gatcgcgttc ggtgctgggg gacgaaacga tgttttttgg gtgaaccggc gtcttcgcat
     2461 ttggcaccac aaaccagtga agcggtacgc aaaccagcaa atgcagcaac gcgaacaccg
     2521 caaatgctcc ccgccagcct atgccggctt ccaggacagt cgtcaatggc cagaatgaag
     2581 tactcgcgaa accggcaatg agggtcaagg ccgtcacgct gcgccggtag tgttcccgtg
     2641 acaattgatg cagggtcgcg aacgccggtt catagagcgt agcggccatg gccacgccac
     2701 ccagcagcca acccgacaag aaaacgacag gatctttggc cagcgacagc gtgagaaaag
     2761 ctgcggcgcc caacagggaa ccggctgtta gaacagttcg gccgccgcgt tcatcgacca
     2821 gtttgcctac cgtcggcgag gctgctcccg agatgagcat gccgatcgtg aacgctccaa
     2881 acagcattgt ctgcgaaatg ccaagagtgg cctgaatcgg tgaagacagt accgcaatgc
     2941 tgtaatacag ggtgccccag gaaatgatct gtgcgatccc gagcgcgggg atgacgcgcc
     3001 aaaactgcac ttttgcctcg gccatgtgat ggccgtttgt ggggatcatc cagccggttc
     3061 gttcattgct gcgggaccgg gcgttgcgtc gacgcagggc gccggaccaa tgcgtgccct
     3121 acctagccgg ccttcttcca gccggatagc tgacgcgcga cttctaccgg tatgggtgcc
     3181 gtaactgcct cttgcggcgg aacgtagttg gtggccggtt cgcttaccgg gaaccgctct
     3241 tcgacgcttt gctcgtggtc tttaagcaac gcttgcaatt cctcgatgct ctgatcgatc
     3301 gttgtagatt tcgcgactgc cggagtcgca gcgcccggcg ctccaacatc gtggccgcag
     3361 taccggcaga cgagcgcctc agccctgatc gattctgcgc aatacaggca ttttttgctg
     3421 gaaccgactt cgatggcttg tgagttttcg ccgggctcga tcagccgtgg ctcgttcatg
     3481 aaatccagtg gagcgggctg cggttcgaca tcccggccgc gcacgtcgcg gttgactgcc
     3541 cagataatgg caccgagcca gccaagcaca gtccatccaa gcgccagatt gatcagtcca
     3601 attgcgggaa tgaaacgatg cctgcggccg gcggcaatga acagcggaat gaaataagcg
     3661 atcagtaaga gcggggctag tacgatgagc cattgctcca tgccgggaaa atatttgctc
     3721 acggcttcag tcatggactt aaggaagctt gaagacgttg gagcgtcgcg cctggacgcc
     3781 ggagcatttg cgaactggaa aactgtcttt cagtgctcga attgccaatg acatttaccg
     3841 cccttcccga gggccaaagt gcatttgtat tccgaatagt gccgaagtgc aagcattggt
     3901 gcgcaaaata cgccgccacg gaacatgtat cgggcccggc cgggcaggcg gttcgcgcag
     3961 tcatttgcta ttcttgatcg actgcaaccc gacccaacac aacgctcatg gcatccaaca
     4021 gttccgctct ccagagtcaa aacattgtgc cgtggcagcc gagccagcgt taccccgatc
     4081 ccgcgatcca gattctcgat cagagttttg cgaggtaccg gttaaattcg gcttcggtag
     4141 aacgcatcgc gcacggcttc cgttggtgcg agggtccggt gtggttcggt gacgggcgct
     4201 atctcttgtg gagcgacatc ccgaacaacc gcatgctgaa gtgggaggag gagaccggta
     4261 tcgtcagcat atttcgcaaa ccctccggat tcgcaaatgg caatacgcgt gaccggcaag
     4321 gccggttaat cacctgcgaa catggtggcc ggcgcgtctc gcgcaccgaa tacgacggca
     4381 caatcacccc gatcgcggac cgcttcgaaa gcaaaccgct caattcgccg aacgacgtgg
     4441 ttgtgaaatc ggatggatcc atctggttta ccgatccgcc gttcgggctg ctgggcaatt
     4501 acgaaggcca cactgccaaa cagcagttgc ccatgaacgt ctatcgcgtc gacggtacca
     4561 gcggagagat caccgtcgtc gaaggcgaag tgaaccgccc caatgggttg tgcttttcgc
     4621 cggacgaatc cattttgtat ctggtggaat cgggcagcac gccgcgcaac atctacgcct
     4681 atgacctcac ggatgacggc aagcggctca cgcgcaaacg caagttcctg gacgcgggag
     4741 ccggtacacc ggacggcatg cgctgtgacg tggacggcaa catctggttt ggttggggaa
     4801 tgggcgacgc agaactcgat ggtgtgatga ttcacaacgc cgatggacga ttgatcggac
     4861 gaatcacgtt gccagaacgc tgcgcgaatt tgtgctttgg cggttccgcc cgcaaccggc
     4921 tgttcatggc agcctcgaaa agcgtctttg cgctatacgt caatacgcag ggagtggccg
     4981 gcggctgatc ggggagcccg aacctatggc cgtaccgcgt gatcggccag cagtttgttg
     5041 agtgtcgaga catcgaccgg ttttgtgata tgcgcgttga agccggcgtt cgcgctgcgc
     5101 accttgtctt cttcctgtcc ccaaccgctg agcgcaatca agaacgtatc cttgccgctg
     5161 gcatgtcccc tgatcctgcg ggccgcgtca taaccgttca gctttggcat accgatgtcc
     5221 atcaacacca catcgggctg gaggtcagcc acggctttca cagcttccag gccatcgcag
     5281 gcagtgtgca cttcatttcc ggttaactgg agcagcatgg ccagtgtcga tgcagcgtcc
     5341 tcattgtcat cgactacgag aatgcgccgc ctgacggagg gctccgtcgg ctcactgccg
     5401 cgttggaccg gcttattttc agaattcggc acgggccaga ttcctgttgt gttgccgagc
     5461 cttcaatacg cgtacaggtc ggaactgcgt gtgcgttgca tgcctgggcg ggtccgacgt
     5521 tgccagttcg tcctcgctcc catccgcctg tcgcgctcgg attattggtg aggtagaagc
     5581 gtcgtcgatt tgcgcgacgt aacccgcatg aggaccaacg cgagcaatat gccgccagct
     5641 attttgagta gcgtagagtt gtccaagatc attctctcca tgagtaggta aggctgcact
     5701 cggcgcctgg tcgcaaactc tgcggcgttg ccgtcagttt cgagaaaagg aactccagcg
     5761 catcctggcg aacaccttcg gtgagtcgtt attttaggtg aaagcgctgg tgaggtggaa
     5821 acaccaacta cggcggtcga caccgatgct caatccaagt tcccgaatcg gaaataatta
     5881 cagggtcgtc agatgcactg atcgtctgct acttcctggc gtccagcaga tgattgcgtg
     5941 tcgcgaagta ggtgatgagc gcggaggtga aggcgcaggc tgccagaaag taaacgccca
     6001 tctcgaaact tttcgtgctg tccttgatcc agccaaccac gaacggtccc acataaccgc
     6061 cgagattacc gatcgagtta atgagcgcaa ttccgccggc cgcggctgcg ccggtaagga
     6121 acaacgaagg catcggccaa aacgatgggc gtgatccgta cagaccaatc gcagcgaccg
     6181 acatggtgac cagcgcccat gcggaaccgg tgaagaaagc cgttcctgcg aggccaatcg
     6241 cggcgagcgt cgatgcgcaa atcagatgcc agcggcgttc gctgtgccgg tctgaggaat
     6301 gaccccagaa gatcagcccg acgacgccaa ccacataagg gatcgcggtg agcagaccgg
     6361 tgatgaaatt ggaatgaccc aagcccttca cgatctgcgg caggaagaaa gtaatgccat
     6421 agccggcaac cacgctggtg aagtaaatca tcgaaagggc gagcacgcgg cggtccgtca
     6481 gcgcacggag cagcgtcagg ctgccggcgg actcaatatt cgcccgttcc agcttgagtt
     6541 ctgtttcaag ccattctttc tcgtcgggct tcagccattt cgccacggct ggccggtctg
     6601 tcatttgcac ccacacgacc agtgcaagca aaaccgcggg aatggcttcg acgatgtaca
     6661 cccattgcca acctttgagt ccgagcaggc cgtccatccc gagcacggca ccggaaacca
     6721 ccgacgccaa cgcattgccg agggggacgg caatgaacaa cgtggaaata acgcgcccgc
     6781 gataccgtgc tggaaaccag taagtgaagt acaggatcat gcccgggaag aacccggctt
     6841 cggctacccc gagcaggaaa cgcaagaaga taaagctggc ggagccggtg gcgacggctg
     6901 tcaaaccgga aacgatgccc caggtgaaca tgatgcgggc gatccaccgc cgagcgccta
     6961 ctttttccag cacgatattg cttgggactt cgaacaacgc ataaccgaga aagaaaatac
     7021 cggcgcccca tccataagca taagcgctca agccgagatc cttgttcatg gtaagtgcgg
     7081 caaaggcgac gttggtgcgg tcgatgtaag ccaccaggta gctcactact accaggggca
     7141 acaagcgcca tgacactttg acaatggttc tgcgccggaa ctctggcgtg aagcgttcgt
     7201 ccatgctttc tcccggatgt tcttattggt ggcagcgctg cacggctggt cgcggcaggt
     7261 tgtgccggcc gattatgctg gacagaacgg tttaagcaaa aactaattgc gcacaccaag
     7321 gcgccggggc cgcgggcgtt catggctacc acatctgcat ggcgcagcct ttctggcgca
     7381 aaaagtttat gcttatgcaa agtcgtgcgc atgggcgcca attcagccca attcttacaa
     7441 atcatttcag gagcacatca tgggcactct caacgtaaat gatagacagg tcaacgtgaa
     7501 tgtcgaaaat gaaatgccgc tgctctgggt gttgcgtgat gagctgggga tgacgggaac
     7561 caagtatggc tgcggcatcg cgatgtgtgg cgcctgcacg gttcacatcg atggcaaacc
     7621 cgtgcgctcc tgcgtcatgc cgatttcagc tgtcaccggt aaagtcacga ccatagaggg
     7681 gctgtcgaga gacggggcac acccgttgca gaaagcatgg gtcaaactgc aggtaccgca
     7741 atgcggttac tgccagagcg gcatgatcat ggcggcggca gccttgctca aggagaacaa
     7801 gcatccctcg gatgcgcaga tcgatgccgc gatgaccaat ctgtgccgct gcggaacgta
     7861 tgtgcgtgtg cgcgaggcca tccacctcgt cgcacagtcc gcgtaaagga gcccatgatg
     7921 aaagcacaaa cattcaatgc cggacgccgc gattttctgg tcaagagcgc tgctgtttcc
     7981 ggaggcctta cgctgggcat ccatttgtcg ggcaacgtac tggccgcaga cgcgccggca
     8041 atcaccgaag tcacccactg gatcgttatc cagcccaatg acgtagtctt aatccgaatt
     8101 gcccgctcgg aactcgggca aggctccttc actggcctgg cgcagctagt cgccgaggaa
     8161 ctcgagtgcg actggagcaa ggtgcgttcg gagtacgccg acgttaacga ccatgtcagg
     8221 cgcaatcgca ttttcggatc catgtccacg ggcggcagcc gttccattcg cgagtcgcag
     8281 gaatatctgc gcaaggccgg ggccagcgcg cgcgaaatgc tggtcgcggc ggcggcgcag
     8341 gaatggggcg tgccggcggc agaatgcaag gccgcgcgca gcgtgatctc gcatccgtcc
     8401 ggcaagagca cgacgttcgg caaggtggcc gccgccgctt caaaactgga agtgccaaag
     8461 gagccgaaac tcaaggaccc caaggactgg aaactaatcg gtacgtcgcc gccgcggttt
     8521 gatatcgcgg acaagaccac gggcaagcag atctatgcag ccgacgtgcg cttgcccggc
     8581 ctgctgcacg catccatcgt tcagtgcccg gtattcggcg gcaagctgaa aagctatgac
     8641 gagtcggcga tccgggatgt aattggcgtc aagcgcgtgg taaccggaga cgactgggtc
     8701 gccgtggtcg ccaacaactg gtggcgcgcc aaccaggcgc tcaaagaact gccggtggaa
     8761 tgggatgtcg gggaaagcgg caaggtcagc agcgattcca tcatgcaatt cctgcgcact
     8821 ggactggatg ctacggacgt gccggtcgcg cgcaaggacg gagatgcgca agcggcgctc
     8881 ggtaatgccg tcaaggtcat cgaggccgaa taccacgcac cttatctgaa ccatgcgacc
     8941 atggagccgc agaccgccac tgcaatagtg acggatgaca aagtcgaagt ctgggtcggc
     9001 acgcagaacg gtgaatcgac cattgccgca gcctcggaag ccgcaggcat tccgctcgag
     9061 aatgtcattg tgcacaagat gcatgcgggc ggcggttttg ggcggcgcgg tccgcatcag
     9121 gagtacacca agcaggccat atggattgcg cagaaaatgc ccggcacgcc ggtgcgtctg
     9181 caatggtcgc gcgaggaaga catgaaacaa ggccgctatc ggccggtcgc actggtgaag
     9241 ctgcgcgccg gactggacaa ggacggcaac tggagcgcct ggcatgtacg tcaggcggat
     9301 caatcgattt tcattaccgt gcgtccggcg gatatcaaga atggcatcga tccggtcaac
     9361 gtgcgatcgt ttcaggacaa tccctatgca gtgccaaatt tcatcgacga gtacgcgatg
     9421 cgcaacacac acgtgccgcc gggtttctgg cgcgccgtgg cgcataccca taatcctttc
     9481 tttcgtgaat gcttcatcga cgaagtggtg catgcggccg gcaaggaccc ggtcgagttt
     9541 cgccgtccgc tgctgcaagg caagaaggac ctgggtgtgc tcgaggcggt agtcaaggct
     9601 gcctcctggg acaagccgcc agccaagggc atttatcgcg gtattgcggt ggtcgattcc
     9661 tatggcagtt tctctgccgc tgttgtggaa atcgcagtga ccaatgcttc agtgatcgag
     9721 gtaaaacgtg tagtcgttgc gctcgatagc ggttacgtgg tgcatcctga tgcagtgctc
     9781 gcgcaggtgc aaagcggcgt aatctggggc ctcagttcgg caatgcatga ggaaatcact
     9841 atcaatgaag gccgcgtcgt gcaaagtaat ttctccgatt accgcgtgct aaccctggct
     9901 gaaacgccca agatcgaatg cgtgctggtg cccaccggtg ggttctgggg cggcgtgggc
     9961 gaaccgccaa tcggcgcagt gattcccgcg ctcgggaatg ccatcttcgc agcgaccgga
    10021 aaacgcgtgc gttcgctgcc gttcaaaaac catggcttca gttataagac atgagcgtga
    10081 atgcgtgaat gtcgtgaata cgtgaatgtc gtcaggtata tgaagctttt ggtttttgcg
    10141 acagacgata ttcacgatag tcacgatcgt cacggcattt tcgccagccg caggcgggcg
    10201 ttgcgggcac tggccgccgg cgcttgcctg ttgacgatca aaccagtgat ggcaacgccc
    10261 gatgaactgg ccgacacgct gcggcaaaca tttggcgacc gggccatcaa tcgcggcaag
    10321 gtaaagcttg agatgccgag gctggcggaa aacggcagcg tggtacccat caccgtcagc
    10381 gtcgacagtg cgatgacgcc gcaagactac gtcaagagca ttcatgtctt cgcgtcggaa
    10441 aaccacttgc cgcgcgtcct cgaagtgcag ctcggtcccc acaatggcaa aggcgtgttc
    10501 tcatcgcggg tccgggtggc gcggtcgcag ccgattactg cggtggcact catgagcgac
    10561 gatacggtat gggcggcggc agtgaatgtc gaagtagtga cttcggagtg cggactgtga
    10621 gccggatggg gaccgcgcgc gtgcgcatgc cggagagcgc gaagcgcggc gaagtattcg
    10681 agatccggac catggtgcag catccgatgg agtccggctt ccggctggat aaccgaggca
    10741 aggaaattcc ccggcacatc gtggagtcct tcgtgtgcaa ctacaacggc aaagagattt
    10801 tccgtgccac gctgcacccg gcggtttcca ccaatccgtt cttcactttt tatgtggtgg
    10861 cggccgagag cggcgaactt tcgtttacct ggcaggatga tcagggaggt gtcgccacgg
    10921 cgacgcagcg gctgactgtt agttaaggag gggcgaacag caagataaac aggcgggtac
    10981 aacgcccgca agaaggtcgt tttctgggct tgtctgaatc aggacctgac cctcggctcg
    11041 ttacttttcg acctcgcttt ccaccttttc tgcgccgctg ttttccacat acttgcgtga
    11101 agttcgtgga tcgagaccgg tgcgtttggc gacttccgag taagtgccaa agcgccgata
    11161 caagagcgcg ctgtatctcg caagaagttc agcagcggtc atgtcgcccg accgcagtct
    11221 ctccagaagc gtctcctctt gtcccagcag cggcaacggg tccgcggtgt aagcgcctgt
    11281 cagcagaatg cgccggactg cctgttcaag ttcccggaca ttgccaggcc acgcatagcc
    11341 ttcaggcaga tttttggtca gcgcacccag cacggtaacg actatgtccg attcctccga
    11401 gcctgtgatg cgtgcaacta ggagctgcac cagatgcttc agttcggcag gagactcggc
    11461 gatgcgctgc cgcagcgtgg gcacatggat gacatcagaa cataggcgat agaagaaatc
    11521 gtcgcggaaa cggccctccc gtcgcaattt ccccaacgac tggttggttg ccgcgatggc
    11581 acgtcccgcg aagcgttttt tctcatgccc gccgagcggg gtgaaggtgc gctcctgcag
    11641 cacttgcaac agtttgatct gcaccgggat cgagacctcg ccaatctcgt ccaggaacag
    11701 cgcaccgtgc gcgctgcacc gggcaaacac gccatcgtga tgatcgatgg ctcccgtgaa
    11761 cgcgcctttg cggtggccga acaattccga ctcgatcagc gcttcaggaa actgcgacaa
    11821 attgatcgag ataaacgtct ccgtgaaatt tgccgcaaat cgtcgctgct cgggaagata
    11881 gggaatgaat cctgatcggc cgatcgccgc agcggcggcg cctttgccgg tgcccgtttc
    11941 gccgagaagc agcgtggaaa agtcctccat gcggttccat agcgtagcct catacccgcg
    12001 catgtcatag gtgaatatgt tgttccacag ggcctcgcgc agccgctgca tggatgggca
    12061 ttcgccagcc agtccgcgat ggatgaaata gaacgcgcgc cgtaactgaa agtagaaggc
    12121 gaaatagcgc acagcccttt cttcctccag accacccccg acgagttgcg caatcgcatc
    12181 gcctgcaaac ggtaccgcga gcgaagcgcc gccctgcatc gcctgtcgct cgatcaagtt
    12241 gtccagctgt ggaacgtatc ggtggtagca aacgtagagg acagccggct ccaggagctt
    12301 gcgatcgtcc gcgctcaatt cgttcacagc cggcatgcct tcgcgcaacc acggcccaag
    12361 tcgaggaccg acgacacgtg ccagcgcttc gcggtcgttc gtcagatcgc cggcttttgc
    12421 gcccggtgcc aggcggacga tcaattcgtc gcgctgcgca ctgaacggat tgccgaaaac
    12481 tacgttgccc aacgcagaaa agaagtcacg gtccgatgaa tttagttttg attttggcat
    12541 gcaataaatg taggtcatcc atacataaaa tgccagagga atataacagt attactggcc
    12601 atttatcata aatgctcctt aaaacaatcg attgagtaat aggcatgctc aatgcaatgc
    12661 acagcatgcc ggcgatgcaa atgacatttc agtgccggac aggcagccaa accttacgga
    12721 gaccagaaat gaaaacgatt acgaagcaaa gcgtgactgc attgttgtcc atagcgatgt
    12781 ttgcaggatg tgcgaccccc aacgggactg atggacaggt ggcaggcaca acgttcgcgc
    12841 agggagtgaa gaacctggtt ctttctcctt tcatgatcgt tgctggcatt gcacagggac
    12901 tcgcgttcct gccttacacg atcggaaccg gtatcggaga gcttaacaag ggcctgctcc
    12961 aggctaacgc ggtgccgctt gatgactcgt acaaggcgac attcggcgtg tctatcgacg
    13021 accagcgcgt ggaccagcaa acgggaaaga ttcagggtca acccgggcaa tatggacgtt
    13081 atcgctcgca ggcgatcttc gaagcaaaca gtgcattgca acgcctgctg gtttcgcagg
    13141 gtatgccgga ggccaaggca aagaactacg tcctggtcgg aaactatcag tacgcgttca
    13201 cccgcggaca gattctgctt gcagtggtgt accggcatcc cggcgaacag cctttccggg
    13261 tgtctgcaaa gcagacgcaa atcgcaacca ccttccggcc gggtcagcgc gggtggcatg
    13321 agccctatca gttcgattcc gagggccatc cactcgatga agtgatcgat tgggcggcaa
    13381 tcgattacaa gctgctgcgt caggaaaaga ttgttgcaac tctcatggtg ctcgcggcgg
    13441 aagcaatcaa atccggcaag cgctcacccg agtactggga cgtcgagaaa ccctggatgg
    13501 ccggcgacac gaccaaagtc atggcgacat cccaagggcg cgtatcgctg ccgtccgcct
    13561 gagcgccgcg ctaaacctca attagacctt cacatttctc atgggtgatc tcatgaaatt
    13621 caaactgaaa atcatcacgg gcctggcgac tttggcgttg ctccatggat gtggaagtgg
    13681 cgatgagttt ggcgaggtca cggtacacac gcaacccggg ccgcctcagg agactcctta
    13741 cgctccgtgt ggcacttaca atggacagcc ggtcgtgtgt cagccactgc gcccc
//
LOCUS       DATMZQ010000531         4970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2363292, whole genome shotgun sequence.
ACCESSION   DATMZQ010000531 DATMZQ010000000
VERSION     DATMZQ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363292"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1808)
                     /locus_tag="VN496_17035"
     CDS             complement(132..1808)
                     /locus_tag="VN496_17035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXF18716.1"
                     /translation="MIFLAGSAVSYAVMGWFTRDIVGRLGGWFAEKSVLYEKARVVQL
                     LVREITLTQKMASSPLLKAWVKNESDPQVRARAIAELEDFRNFFRSKSYFFAIARSGN
                     YYFNDERGGADLYRPRYTLSPSIAKDGWFYATLDKVKDYELNVDTDRHLKVTKVWINT
                     VLRDEGKPLAVIGTGVDLSDFIESVISTSQPGVTNVLLDKNGAIQAHRDVSIIDFASI
                     AKEQRNEQQSTLFNLIDDAHQRTAVQEALMDLSIGKGETRTLQVSIQGKRHIAGIAYL
                     PDIKWFIVSLAHPEAAENHNYLVTMIAIRIGGLALMLLIVGIAFDRMVLRRLALLDVA
                     AKEVSAGNYSVQLPHGGSDELSRLGQTLQDMTERVAAHTTNLEHQVAERTRVLAQQAQ
                     ADFLTGLLNRRGMGERIEVEKNRLAREGAKLGMLILDLDHFKQINDTHGHDVGDQALV
                     QIARTIRNVMRSYDLCSRWGGEEFLVIAPHIVSRAALIIVAEKLRAEIKSKPVPIPGG
                     KLNLTVSIGGYFADPQESTDTIVKAADDALYLAKQGGRDRTVVTERPELA"
     gene            complement(2069..3652)
                     /locus_tag="VN496_17040"
     CDS             complement(2069..3652)
                     /locus_tag="VN496_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18717.1"
                     /translation="MNASKPAIWVPGDWNALFGFGTNILLNLLVLTGLLRYVLKMPDA
                     LVFGRILPACGLMLFMSTMYYAWLAYQLARKTGRNDVTALPSGTSVPHMFVVVFVIML
                     PIAIKTGDPVKGWEAGLAWVFIQSFVLMLGGFIAPWIRKVTPRAALLGTLAGVSIAFI
                     SMRPALEMFMTPLIGIICFAIILASWLGGVRYFKGIPAGLVAIAVGTLIAWGSTALGL
                     NYGGMGLDKLAGSISNFGFSIPMPALGHVFGGFEFLGVILVTAIPFGIYDLVEAIDNV
                     ESASTAGDSFPTTRVLTADGRISLIGCLMGNPFILAVYIGHPGWKSIGGRIGYSAATG
                     ILVILLAWFGIISVMMALIPVVAIAPILLYIGMLIGSQAFQETPRSHAPAIILGLVPH
                     VAAWGKLQIDGALGAAGTNAAAIGFDKLGQVGVLYQGLQVIGGGAILGGLVLTSIAVF
                     VIDRAFNKAALFALIGAAFTFFGFMHGEAIGFAQSPVVALAYLGIAGVMFGCARYAAA
                     APQPAQQVHPHGPVGQLAT"
     gene            complement(3967..4497)
                     /locus_tag="VN496_17045"
     CDS             complement(3967..4497)
                     /locus_tag="VN496_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HXF18718.1"
                     /translation="MFKQALGSCLLVIAAVSMMSAAQAEEKENPGACRADVQKLCKGV
                     HPGGGRIAACLKQHESEISPGCKENMAKAREEVKELAEACRADAETLCKGVKPGQGRV
                     MRCLAEHKDKVSSACNAKLAEEQSRHPCMADMQRLCKDVQPGQGRMMECMKKHESELS
                     PECKAHHAQQKSSDKQ"
     gene            4808..>4970
                     /locus_tag="VN496_17050"
     CDS             4808..>4970
                     /locus_tag="VN496_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXF18719.1"
                     /translation="MNRIRQGGFTLIEIAIVLVIIGLLLGGVLKGQELINNARVKSFA
                     NDFRNIPVYI"
BASE COUNT         1033 a         1463 c         1427 g         1047 t
ORIGIN      
        1 ccctcgcgca gttcttccag cacgctaatc atgcgaccgg cttcgttatc attcatcgcg
       61 gcagtccgcc caccgacccg acagtttgcc agccgcgggc tgcatcagta cagttggacg
      121 caaacgcctg gtcaagcaag ctccggcctt tccgtgacga ccgtgcggtc gcgacctcct
      181 tgcttggcga ggtagagcgc atcgtcggcg gccttgacaa tcgtgtcggt gctctcctgt
      241 ggatcggcga aatatcctcc aatgctgact gtaagattca atttgccccc tgggatcggt
      301 accggcttgg atttgatttc tgctctgagc ttttctgcca cgatgatcag cgctgcgcgg
      361 ctgacgatat ggggtgcaat gaccaaaaat tcctcaccgc cccagcgcga acacaagtcg
      421 taggagcgca tcacgttgcg aatggtccgg gcaatttgca ccagcgcctg gtcgcccaca
      481 tcgtgtccat gcgtgtcgtt gatctgtttg aagtgatcga ggtcgaggat cagcatgccg
      541 agtttcgcgc cctcgcgcgc caaccggttc ttttcgactt cgatgcgctc acccatgcca
      601 cggcggttca gcaacccggt caggaaatca gcctgggcct gttgtgcaag cacgcgggtg
      661 cgttccgcca cctgatgttc cagattggtc gtgtgggcag cgacacgttc ggtcatgtcc
      721 tgcagcgtct gcccgagccg gctcaattca tcgctgccgc catgcggaag ctgcacggag
      781 taattaccag cggaaacttc ctttgccgcc acatcgagca gcgccaaacg ccgcaacacc
      841 atgcggtcga acgcgatccc gacgatcagc agcatcagcg ccaaaccgcc aatccggatt
      901 gcgatcatgg tcaccagata attatgattc tcggctgcct ccggatgcgc gagcgaaaca
      961 atgaaccatt tgatgtccgg cagataggct atgccggcga tgtgccgctt gccctggatt
     1021 gaaacctgca gcgtgcgtgt ctctcccttg ccgatcgaca agtccatgag cgcttcctgg
     1081 accgccgtgc gttggtgcgc atcatcaatc agattgaaca gcgtgctttg ctgctcgttg
     1141 cgttgctcct tggcgatcga cgcaaaatcg atgatcgaga cgtcacggtg tgcctggatg
     1201 gccccgtttt tgtcgagcaa cacattggtc acgcccggtt gcgacgtgct aatgacgctc
     1261 tcaatgaaat ctgacaggtc cactcccgtg ccgatcactg ccagcggctt gccttcgtcc
     1321 cggagcacgg tattgatcca caccttggtt accttgaggt gccggtcggt gtccacgttc
     1381 agctcgtaat ccttcacttt atctagcgtg gcgtagaacc agccatcctt ggcgatcgac
     1441 gggctgagcg tatagcgggg ccgatacaag tctgcgccgc cccgctcgtc gttgaaataa
     1501 tagtttccgg agcgcgcaat ggcgaagaag taactcttgc tccggaaaaa attccggaag
     1561 tcctcgagct ccgcaatcgc ccgggccctg acctgcggat cggactcatt tttcacccaa
     1621 gctttgagca gcggcgagct ggccattttc tgggtcagcg tgatttcgcg gaccagcaac
     1681 tgaacgacgc gcgctttttc gtagagcacg cttttctcgg cgaaccagcc gcccagcctg
     1741 cccacgatat cgcgggtgaa ccatcccatg acggcgtaac tcactgccga gccggccagg
     1801 aagatcacga tgaaaaggac cagaaatttg tttcgcagcg aggcgacgcc cttttttctc
     1861 ctgctgtatt cggtgcttga agtctgctgt accaagtgcc cgcctccatg agcttcatga
     1921 tgaagccgtc gcggaacagg agggtagtgg attttcagcc cgctgtcgcg ggctggcggg
     1981 attttcgccc gcccgatgac gccggttgaa atgccttgag cccggtacga gcaccgggct
     2041 caagcaagca aacgcagaat ggttagcgtt aagttgccaa ctgcccgacc ggtccgtgag
     2101 ggtgaacctg ctgtgcaggc tgcggcgcgg cggcggcata ccgggcgcag ccaaacatca
     2161 cgcccgcaat gccaaggtat gcaagcgcga ccacgggcga ttgtgcaaag ccgatggcct
     2221 cgccgtgcat gaagccgaaa aaggtaaatg ccgcgccgat cagcgcaaac aaagccgcct
     2281 tattgaatgc gcgatcgatc acgaatacgg cgatggaggt cagcaccagg ccgccgagga
     2341 tggcgccgcc accaatgact tgaaggccct gataaagcac acccacttga ccaagcttgt
     2401 caaacccgat tgctgcagca ttggtgcctg ccgcgcctaa ggcgccgtca atttgcagct
     2461 tgccccacgc cgcgacgtgc ggcactaggc ccaggatgat tgcaggcgca tggctgcgag
     2521 gagtttcctg aaacgcctgc gagccgatga gcatgccgat gtacagcagg atcggggcga
     2581 tggctacgac cgggatcagc gccatcatca cggagatgat gccgaaccat gccaagagga
     2641 ttacgagtat gccggttgca gcggaatagc caatgcgccc accgatcgat ttccagcctg
     2701 ggtggccaat gtaaacagca aggatgaacg ggttccccat caggcagccg atcaggctga
     2761 tgcgaccgtc agcggtcagc acgcgcgtag tggggaagct gtctccagca gttgacgcgc
     2821 tttcgacgtt gtcgattgct tccaccagat cgtatatgcc gaatgggatc gcggtcacca
     2881 aaatgacgcc cagaaattcg aatccgccga acacgtggcc gagggccggc atcggaatcg
     2941 aaaagccgaa attggaaatg gagccggcca gtttgtcgag gcccatgccg ccatagttga
     3001 ggcccagtgc ggtagatccc caggcgatga gtgtgccgac cgcgatggca accaatcctg
     3061 cgggaatgcc tttgaagtag cgcacgccgc ccaaccagct cgccaggatg atcgcgaagc
     3121 agatgatgcc gatcagcggc gtcatgaaca tctccagcgc gggacgcatg gaaatgaatg
     3181 cgatcgatac gccggccagc gtgccgagca gtgcggcacg tggcgtgacc ttgcgaatcc
     3241 agggcgcgat aaatccaccc aacatgagca cgaaactttg gataaatacc caggccaacc
     3301 cggcttccca gcccttcacc ggatcgccgg tcttgattgc aattggcagc atgatcacga
     3361 agaccaccac gaacatgtgc ggcacgctgg tgcctgacgg tagtgcggtt acgtcattgc
     3421 gtccggtttt ccttgccagc tgataggcaa gccaggcgta gtacatcgtg ctcatgaaca
     3481 gcatgaggcc gcaggctgga aggatgcggc cgaacaccag cgcatccggc atcttcagca
     3541 cgtagcgcag caggccggtg agcaccagca ggttgagcag gatgttggtg ccaaatccga
     3601 atagggcgtt ccagtctccc ggtacccaga tggcaggctt gcttgcattc atgatcggct
     3661 cccttgcgtg gttggctggc gtagtcgcgg cgcaatgcat gccagcgcaa taagcggacc
     3721 accacgcttc gagatgccga tcgctttcaa ttcagaccgt tgagtcgaca acaggcttac
     3781 ggccgcacgg cagcggcacc ggaacggtga accgctgccc gatgctagtg catcgcttgc
     3841 tgtttctgtt tctgtatggc cgccagatga tcaagaccaa acggggacgt cacgatcagg
     3901 attagcgcac cccaatcggc gatacgttca aagttttccg gacacgttcg attgtgctga
     3961 gtgtgcttac tgcttgtcgc tgcttttttg ctgcgcgtgg tgtgccttgc actccggcga
     4021 caattcagat tcgtgcttct tcatgcactc catcattcga ccctgccccg gttgaacgtc
     4081 tttgcacaga cgttgcatgt ctgccatgca ggggtggcgg ctttgttctt cggccagttt
     4141 tgcgttacat gcactggaca ctttgtcctt atgttcggcc aggcagcgca tgacgcggcc
     4201 ttgcccgggt ttcacgccct tgcagagcgt ctcggcatct gccctgcagg cctccgccaa
     4261 ctcctttact tcttctcgcg ccttcgccat attttccttg cagcctggcg aaatttcgga
     4321 ctcgtgttgt ttcaagcagg ccgctatgcg gccccctccg ggatggacgc ctttgcacag
     4381 cttttgcaca tccgcccggc aggcgccagg gttttccttt tcttcggcct gcgcggccga
     4441 catcatggat actgcagcga tgaccagcag acaggagccg agtgcttgct tgaacataag
     4501 tcctccagga gttggtcggc gcattgccga cagggcgtaa acgattgtcc gggctgagac
     4561 gttgacgacg gcgctggtaa atcgacgtgc gccaacgcgg tttgcaatca accggcccaa
     4621 tttcatggac gttatcgtgg tattttacga tctgccgaca agttgtcaga acggtcgaaa
     4681 aacgcaacga ccgcataata agattcgtcg tttagagtgc gaaaccaggg cgggaacgcg
     4741 atcactgctt gtggcacgct tctggcaaaa gacgcaccgc gcatgtaatt acaaataagg
     4801 ggaagagatg aatagaatta ggcagggagg attcacactg atcgaaatcg ccatcgtgct
     4861 ggtgatcatc ggtcttctgt tgggaggggt gctcaaaggt caggaactga tcaacaacgc
     4921 ccgggtaaag agtttcgcga acgacttccg caacatcccg gtttatattt
//
LOCUS       DATMZQ010000532         9474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5127455, whole genome shotgun sequence.
ACCESSION   DATMZQ010000532 DATMZQ010000000
VERSION     DATMZQ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5127455"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VN496_17055"
     CDS             complement(<1..1122)
                     /locus_tag="VN496_17055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HXF18720.1"
                     /translation="MRRIAILVGTLLAGQAFAADLLTIYREALIQDSTYAGAKAQYIG
                     TQERLPQARALLLPNINFGAGTHYNEVNADYPNNPALPSGRFNFYDYNYGVNVTQPLY
                     RRQNDAIYEQAKVQVRQAQNQLSAANQDLITRVAQSYFDVLLARVNLSTIRSQKSAVA
                     EQLEQAKRNFIVGTATITDSREAQARYDLVVAQELVSENDLEVKTRALELIVGKPVGN
                     LAGLGAPVTLNPPEPADMGTWVEQAYQSSLQIALARQNVEIAAQEIRKADAGNYPTLD
                     AVGSLTDNYASNSVIGIRSDLRSLIVGVQLNIPLYQGGGISSRVREAVAGQERARQDL
                     ENARRTVAQQTREAFLGVTSGLGQIKALRQAVGSTTLQLE"
     gene            complement(1119..1757)
                     /locus_tag="VN496_17060"
     CDS             complement(1119..1757)
                     /locus_tag="VN496_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXF18721.1"
                     /translation="MSAALDLFVERGFAATRLDEVAARAGVSKGTLYLYFSSKEELFK
                     AVIRSGIVPLIERGERLLDEHRGTCSELLRKIVFNWWTSVGTTQLGGIPKLMFSECRN
                     FPEIGKFYYEEVISRGHLLVQTVLERGMRSGEFRRMDANNAMRMILAPLVFLLLWRYS
                     FDFCDSKRVDPEKFLDQHLDMVVNGLAPSVREQKKSIEPATIISIAKKGKNR"
     gene            2037..3410
                     /gene="amrB"
                     /locus_tag="VN496_17065"
     CDS             2037..3410
                     /gene="amrB"
                     /locus_tag="VN496_17065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXF18722.1"
                     /translation="MSAVRAPAVAGSFYPYAAAELQAQLDGLLSSTPSTEPAMPKALI
                     VPHAGYIYSGSIAASAYAALRPFRDKIERVVLLGPTHRVAVRGLALPASDAFATPLGL
                     VAVDTNAATALRAMTQVCTSEQVHALEHSLEVHLPFLQRVLHRFKLVPLAVGDASTED
                     VAQVLNALWGGEETLIIVSSDLSHYLPYEQARRVDEQTAGMVLGLSPQLDHQRACGAT
                     PINGLLRVAGERGLRPQLLDLRNSGDTAGDRNRVVGYASFAFYPAAVRVQQESGTADS
                     AIDGKLLVPIARASIASQFGLHFSVRASAAFLHEPGASFVTLKSNGELRGCIGSLTAN
                     RNLVDDVRANACAAAFKDPRFAPLRLDEFGSIRVEVSLLSALEPMSFKTEADALEQMR
                     PGVDGMMLEFGAHRGTFLPQVWENLAEPQSFLGQLKRKAGLPVDFWDTGVQLSRYTVT
                     KWSESSS"
     gene            3407..4492
                     /gene="amrS"
                     /locus_tag="VN496_17070"
     CDS             3407..4492
                     /gene="amrS"
                     /locus_tag="VN496_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXF18723.1"
                     /translation="MTISEQAFHPGRWWHMLDDGRMQCDLCPRDCRLHEGQRGACFVR
                     QRIDNKMVLTTYGRSTGFCVDPIEKKPLNHFYPGSSVLSFGTAGCNLACKFCQNWDIS
                     KSRDMDRLMDLAQPEQIAAAAQRAGSKSVAFTYNDPVIFAEYAIDVAQACHEREIKTV
                     AVTAGYMHDVPRREFYAHMDAANVDLKAFTDEFYVKQTGSHLQPVLDTLKYLRHETDV
                     WLEITTLLIPTKNDSDEELYQLSKWVATELGGEVPLHFTAFHPDYKLDDLPATPASTL
                     TRARQIARGEGLQYVYTGNVHDNEGGTTYCPGCGGALVVRDWHRILAYSISAEGRCGS
                     CGTPVAGRYGEFDKPFGARRIPISITA"
     gene            complement(4527..5426)
                     /gene="folD"
                     /locus_tag="VN496_17075"
     CDS             complement(4527..5426)
                     /gene="folD"
                     /locus_tag="VN496_17075"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXF18724.1"
                     /translation="MSAKLMDGVAVSTAIRAQWKTRAEALKAKGVTPGLAVILVGDNA
                     ASQVYVRNKAKACTEMGLHSEVHHFAKDVAEQTVLGKIAELNQSSKIHGILVQLPLPQ
                     QIDNHKVLEAISVEKDADGFHLHNLGALVTGSTDFPPCTPYGVMTLLDFYGIALEGQN
                     AVVVGRSNIVGKPMALMLLARSATVSICTSKTRDLKQYTSMADILVVATGRPKMITGE
                     FIKTGAVVVDVGINRLADGRLVGDVDFDGASSKASYITPVPGGVGPMTITMLLANTVR
                     AAERIAAKTLKQVESGREVFLQR"
     gene            complement(5478..7583)
                     /locus_tag="VN496_17080"
     CDS             complement(5478..7583)
                     /locus_tag="VN496_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXF18725.1"
                     /translation="MGVIDEAGISNKVRTVRVACPHDCPDGCSMLVTVDNASGRAIKV
                     AGDPAHPVTRGYLCNKVNHYLDLVYNDKRVLYPHRRVGPKGADAKFVRIGWDEALTEI
                     AARLKAVIAQYGTEAVQPFSYSGTLGMLGFFGMGERFFNKMGAARLERTICTAAGAAA
                     EMHTFGRVGDANIEDLPEMKVVILWGTNLVSTGVHAMPFVTAARDNGAQIIAIDPRVT
                     RTTAFADWHLQPRPGTDAALALGIMKVIVDRGLHDEDFLKRNTVGWEQLLQRLGDYPL
                     GRVESITGIPTADIEKLALLYGGTRQSFIRLNWGIQRHDNGGMMTRAIKLLPIVTGAV
                     RGKGGVCMSTGGEMRRVDMRKLQATYLLEGRTPRTVNMIQLGNALNDPALDPPVKALF
                     CWSADPANCVPDTIAARKGLAREDLFTVVHDTFWSDTAAYADIVLPADTALEHADLLP
                     AYGNYYYSLSEQAIEKQGESLDNQELFRRLAKAMGYDEPCFSQSDEDMIRELMDPEFN
                     PLFEGITYEGLKRDGWAKANVDSPRRWGVNSGRWPTPSGKIEIYSQALAKTGVDPLPM
                     HVPEQEGFESTELKKKFPLQVISAATHYFIGASFQHVPRLQDMLARPTFEISVADAKA
                     RGIEDGDYCRLFNDRGETFGYALIVEGLLTGVLGAQKQLRGSRMRGGVNINALTSQRE
                     ADMGRGPVFFSTLAQLEKA"
     gene            7694..>9474
                     /locus_tag="VN496_17085"
     CDS             7694..>9474
                     /locus_tag="VN496_17085"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HXF18726.1"
                     /translation="MRYVRTCSNVVLAAALSLCANVNAGLVKLEIAKRELVADGAGFG
                     SAGPYEKLTGRAWFEIDPALERNKAVFDIDHAPLNASGKVEFSADMVILKPVDMEKSS
                     RTLFFEVNNRGRKISFGRMQDTAADANMNDPMAARDFGNGFLMKRGYVVAWVGWGADI
                     APGDSRLTVTFPISQENGRPVTERILTEFSDRNFNGGKPTSLPLSGSTAFKSYAAVSS
                     DKKLAHAELYALPSDSLKSSTADIPRGEAIPDDQWALADCPDGWPGQPSSQHICLKEG
                     FRNDRNYHLIYRATGSPVMGLGYLTTRDFVSFLKNDERDKSGNPNPVFGIKTTLCQGI
                     SSSGMYLRDYLYQGFNVDEQERQVCEGMHIHVAGVQKLYLNYRFAQPNPFTQQHRERY
                     VPDTNFPRQYAVRINPFTRVPDGILKRPKTDPKIIHTDTSNEYWQFRASLTGSSEGGT
                     MDFNESSKVRRYLLAGLQHGGFKSDKPNHGLADKQCEQLTNTTHPRVLLHTLIIVLDE
                     WIINNVTPPKTRVPKISDKTLVTPAELNLPKIPGFAYEGLYNGSGDRDFGPRVTGNSG
                     VIDLLIPKTLAPHKILVPQVDAIGNDIA"
BASE COUNT         1948 a         2831 c         2641 g         2054 t
ORIGIN      
        1 ttcgagctgc agcgtggtcg agcccaccgc ctggcgcagc gccttgatct gccccagccc
       61 cgaggtcacc cccaggaacg cttcgcgggt ttgctgcgcc acggtgcgcc gcgcattttc
      121 caggtcctgg cgcgcgcgct cttgtccggc cacggcttcg cgcacgcgcg agctgatgcc
      181 cccgccctgg tagagcggga tgttcagctg caccccgacg atcagcgagc gcaggtcgct
      241 gcggatgccg atcacggagt tgctcgcgta gttgtcggtg agcgagccca cggcatcgag
      301 cgtggggtag ttcccggcat cggctttgcg gatttcctgg gcggcaattt cgacgttttg
      361 ccgcgcgagc gcaatttgca ggctggactg gtaggcctgt tcgacccagg tgcccatgtc
      421 ggcgggctcg ggcgggttca gtgtcaccgg tgcgcctaag cccgccaagt tgcccacggg
      481 cttgcccacg atcagttcga gcgcccgggt tttgacttcc aggtcattct cgctcaccag
      541 ttcctgggcc accaccaggt cgtagcgcgc ctgggcttcg cgcgaatcgg tgatggtggc
      601 cgtacccacg atgaagttgc gcttggcctg ttcgagttgt tcggccacgg cgcttttttg
      661 cgagcggatg gtgcttaggt ttacccgtgc gagcagcacg tcgaagtagc tctgtgcaac
      721 gcgcgtgatc aggtcctggt tggccgccga gagctggttc tgggcctggc gcacctgcac
      781 cttggcctgt tcgtagatgg catcgttttg ccgccggtac agcggctggg tcacgttaac
      841 cccatagtta tagtcgtaga agttgaagcg cccggagggc agtgccgggt tgttggggta
      901 atcggcattg acttcgttgt agtgggtgcc tgcgccgaag ttgatattgg gcagcagcag
      961 cgcgcgcgcc tgcggcaggc gctcctgtgt tccgatgtat tgggcctttg cccccgcgta
     1021 agtggagtcc tggatcagcg cttcacggta gatcgtcaac aggtcggcgg cgaaggcttg
     1081 cccggctaac aatgtcccga ccaatattgc aatgcgcctc atcgattttt ccccttcttt
     1141 gcgatgctga ttattgttgc cggctcaatg ctcttttttt gctctcgcac gctgggagcg
     1201 aggccattga ccaccatgtc cagatgctga tccagaaact tctccggatc cactcgcttg
     1261 ctatcacaga aatcaaatga ataacgccac agcagcaaaa aaaccagcgg tgccagtatc
     1321 atcctcatgg cattgttcgc atccatacgt cggaactcgc cgcttctcat gcccctttcc
     1381 agcaccgtct ggaccagcag atgcccgcgg ctaatgactt cttcataata aaatttgccg
     1441 atttcgggga aattccggca ttccgagaac atcaatttcg gaattccacc cagctgcgtt
     1501 gtgccgaccg acgtccacca gttgaagacg atcttgcgca gcaattccga gcaggttccg
     1561 cgatgttcat ccagaaggcg ctcgccgcgt tcgatgagag gaacaatgcc ggaacggatg
     1621 actgccttga acaattcctc tttgctcgaa aaatagagat agagtgttcc cttgctgacg
     1681 cctgcgcggg cagccacctc atcgagacgg gtcgcggcaa aacctcgctc cacgaataaa
     1741 tccagtgctg ccgacatcaa ttcggctggc cttgcctgtt tacgccgcgt ccaccggcgg
     1801 tcttgtttgg gttggggatt cacgaaatct tataatatct taatgactga gcggtcagta
     1861 atatacacga ttgcgactgc gccaaacact aaataatgag ccaaatcctt tgtttgaatt
     1921 acccgaaacg ttacagcgcc aaacacgttc gaggcataca aaaattacgg gagtcgcctt
     1981 gatggtgttg cgttgaaccc cacctacgca gtattggaac aattggaaac acggccatga
     2041 gtgcagtcag ggcaccggca gtcgccggta gcttttatcc gtatgccgcc gccgaattgc
     2101 aagcgcaact cgatggcctg ctgtcctcca cgccgtccac agagccggcc atgccaaagg
     2161 ccctgattgt gccgcacgca ggctatatct attccggatc gatagccgcg agtgcgtatg
     2221 ccgcgctgcg tccgttccgg gataagatcg agcgggtagt cctgcttgga ccgacacatc
     2281 gtgttgccgt aagaggactc gcgttgcccg catcggacgc atttgcgaca ccccttggcc
     2341 tggttgccgt ggacaccaac gccgccacgg cgttacgcgc catgacgcaa gtctgcacca
     2401 gcgagcaagt gcatgcgctc gagcactctc ttgaagtcca tttgccgttc ctgcagcgcg
     2461 tacttcatcg cttcaagctg gtgccgctcg ccgtcggcga cgcatcgaca gaagacgtcg
     2521 cgcaagtatt gaatgcctta tggggcggag aagaaacact cattatcgtg agttccgacc
     2581 tgtcccatta cctgccttac gaacaagcac ggagggtcga tgagcagacc gctggcatgg
     2641 tacttggact ttccccgcag ctggatcatc aacgcgcctg tggcgccacg ccgatcaatg
     2701 gtctgctgcg tgttgcgggc gagcgcggat tgcgcccgca gctgctggat cttcgcaatt
     2761 cgggggatac ggccggcgac cgcaatcgtg ttgtgggcta cgcgtcgttt gccttctatc
     2821 cggctgcggt ccgcgtacag caggaatccg gcacggcgga ttccgccata gacggaaagc
     2881 tgctggtgcc catcgcgcgc gcgtccatcg caagtcagtt cgggctgcat ttttcagtgc
     2941 gagccagcgc ggcttttttg catgagccgg gcgcatcatt cgtgacgctc aaaagcaacg
     3001 gtgagttgcg gggctgcatt ggcagcctga cggctaaccg caacctggtg gatgatgtgc
     3061 gtgccaacgc gtgtgcggcc gccttcaagg atccccggtt tgcgccgtta cgccttgatg
     3121 agttcggctc gattcgggta gaggtctctt tgctttccgc gcttgagccc atgagtttca
     3181 agaccgaggc agacgccctc gagcagatgc gaccaggggt ggatggcatg atgctggaat
     3241 tcggtgcaca tcgaggcact ttcctgccgc aggtctggga aaacttggcc gaaccgcaaa
     3301 gttttctcgg tcaactcaag cgcaaggcgg gcctaccggt tgatttctgg gatactggcg
     3361 tgcaactttc ccgctatacg gtcaccaagt ggtcggagtc ctcctcatga ccatttcaga
     3421 acaagcattt caccccggac gctggtggca catgctggat gacggtcgca tgcaatgcga
     3481 cctgtgtccg cgcgactgca ggttgcatga aggccagcgt ggcgcctgtt tcgttcgtca
     3541 acgcattgac aacaaaatgg tcctgacgac gtacggccgt tcgaccggat tctgcgtcga
     3601 cccgatcgaa aagaaaccgt taaatcattt ctacccgggt tccagcgtgc tttcgtttgg
     3661 cactgcaggc tgcaatctgg catgcaaatt ctgccaaaac tgggacattt ccaaatcgcg
     3721 tgacatggat cggctcatgg accttgcgca gcccgagcag attgccgctg ccgcgcagcg
     3781 ggcgggcagc aagagcgttg ctttcactta caacgatcca gtgattttcg cggagtatgc
     3841 gatcgacgtc gcccaagcgt gtcacgagcg cgaaatcaaa acggtcgccg tcacggcagg
     3901 ctatatgcac gatgtcccgc gtcgggaatt ctatgcccac atggatgctg cgaacgtcga
     3961 cttgaaagca tttactgatg agttctatgt aaaacagact ggatcgcatt tgcagccggt
     4021 gctcgacacg ctgaagtatt tgcgccacga aacggatgtc tggctggaaa tcacaacgct
     4081 gctgattccg accaaaaacg attcagatga ggagctctat cagctttcca aatgggttgc
     4141 cacggagctt ggcggcgagg tgccgctcca cttcaccgcc tttcatccgg attacaagtt
     4201 ggatgatctg cccgccacgc cggcctccac actgacgcgg gcacgccaaa ttgcgcgcgg
     4261 cgaaggattg caatacgtct ataccggcaa cgtacacgac aacgaaggcg gtacaacgta
     4321 ttgtccaggc tgcgggggtg ctttggtggt gcgcgattgg caccgcattt tagcttacag
     4381 catttccgca gagggccgct gcggtagttg cggcacacct gttgccggac gttatggcga
     4441 gttcgacaag ccgttcggag cacggcgcat cccgatctcc attaccgcgt gaggcacatg
     4501 cctcggagta ccgacgcggc acctatttac ctttgcagga aaacctcgcg accgctttca
     4561 acttgcttga gcgttttggc ggcaatcctt tcagccgccc gcacggtgtt ggccaacaac
     4621 atggtgatgg tcatgggccc aacgcctccg ggaactggcg tgatgtagga agccttgctg
     4681 cttgcgccgt caaaatccac atcgcccacc agcctgccgt ccgctaaccg gttaatgccc
     4741 acatcgacga ctacagcgcc cgtcttgatg aattccccgg taatcatttt cggcctgccg
     4801 gtcgcaacca ccaggatgtc tgccatcgag gtgtattgtt tcaggtcgcg tgtcttcgat
     4861 gtgcagattg acacggtggc gctgcgcgcg aggagcatga gggccattgg cttgccaacg
     4921 atgttgctgc ggcctaccac gacggcattc tgcccttcca gggcaatgcc gtaaaagtcc
     4981 agcagggtca tcacgccata cggcgtgcac ggcggaaaat ccgtggagcc ggtcaccagg
     5041 gcgccaagat tatggagatg gaagccgtcc gcatccttct ccacggaaat agcttccagc
     5101 accttgtggt tgtcgatctg ctgcggcaat ggcagttgca ccagaatgcc gtggattttg
     5161 ctgctctggt tcaactcggc tattttgcca agcaccgtct gttcggcaac atctttcgcg
     5221 aaatggtgca cttccgaatg cagtcccatt tccgtgcagg ctttggcctt gttgcgaacg
     5281 taaacctgtg acgctgcatt gtcgccgaca agaatgaccg ccagaccggg tgtcacgccc
     5341 ttcgctttca acgcttccgc gcgcgttttc cattgcgcac gaatcgcagt ggacaccgca
     5401 acaccgtcca ttaacttggc gctcacggct cgcttccgcg gcttatcgtc ttgagcaaca
     5461 atactgacgc aggctattca agccttctcc agctgtgcca gtgtggaaaa aaacactggt
     5521 ccacgcccca tatccgcctc gcgctgactg gtcagcgcat tgatgttcac gccgccgcgc
     5581 attctggagc cccggagctg tttttgcgcg cccagcactc cggttagcaa accttccacg
     5641 atcagcgcat acccgaatgt ttcaccgcgg tcattgaaca gcctgcaata gtccccgtcc
     5701 tcgatgcccc gcgccttcgc gtccgcgaca ctgatctcaa aagtcggacg cgccagcatg
     5761 tcctgcaaac gaggcacgtg ctggaaactt gcaccgatga agtagtgtgt tgccgcgctg
     5821 atgacttgca gcggaaattt tttcttcagc tcggtgctct cgaaaccttc ctgctccggc
     5881 acatgcatcg gcaggggatc gacgccggtt tttgcaagcg cttgcgaata aatctctatt
     5941 ttgccggacg gagtcggcca tcttccgctg ttaacacccc agcggcgcgg agaatcgaca
     6001 ttggccttag cccatccatc ccgcttcaat ccttcataag taatgccttc aaacagcgga
     6061 ttgaattctg gatccatcag ctcgcgaatc atgtcttcgt cggattgcga gaaacacggc
     6121 tcgtcgtagc ccattgcttt cgcgagccgg cggaacaact cctggttatc caggctttcg
     6181 ccctgcttct ctatcgcctg ctcggacagg gagtagtaat aatttccgta agcaggcagc
     6241 agatccgcgt gttcaagtgc ggtatccgca ggcagcacga tatccgcgta agccgcggtg
     6301 tccgaccaga aggtgtcgtg caccaccgta aacagatcct cgcgcgcaag acccttgcgg
     6361 gccgcgatag tatcaggaac gcagttcgcg ggatccgcgc tccagcaaaa caacgccttt
     6421 accggcggat ccagagcagg gtcattgagg gcgttaccca gctgaatcat gttcacggtt
     6481 cgcggcgtgc gaccttcgag caaataagta gcctgtagct tgcgcatgtc gacgcggcgc
     6541 atttcgccgc ccgtggacat gcatacgccg cctttgcccc gcacggcgcc ggtcacgatg
     6601 ggtaacagct tgatggcgcg cgtcatcatg ccaccgttgt cgtggcgctg gattccccag
     6661 ttcaggcgaa tgaaggactg tcgcgtgccg ccgtagagca acgccagttt ttcgatatcg
     6721 gcggtcggaa tgccggtaat tgattcgacg cgcccgagcg ggtagtcgcc cagacgttgc
     6781 agcaactgtt cccagccgac ggtattgcgc ttcaggaagt cctcgtcgtg cagtccgcgg
     6841 tcaacgatga ccttcattat gccgagagct agcgccgcgt cggtgccggg ccgaggctgg
     6901 agatgccagt ccgcaaacgc ggtggtgcgc gtaacacgcg gatctatggc aatgatctgc
     6961 gcgccgttat cgcgcgccgc agtcacgaac ggcatggcgt gcacgccagt ggataccagg
     7021 ttcgttcccc acaggatcac cactttcatt tccggcagat cttcgatatt ggcgtcgccg
     7081 acccgtccga aagtatgcat ctcggcggca gcgccggccg ccgtacaaat ggtgcgctcc
     7141 agccgagccg cacccatctt gttgaagaag cgctcgccca tgccaaaaaa accaagcatg
     7201 cccagcgtgc ccgaatagga gaacggctgc accgcttcgg tgccgtattg ggctattacg
     7261 gcctttagcc gcgctgcaat ttcggtcaga gcctcatccc agccaatgcg cacgaatttc
     7321 gcatcggccc ctttggggcc gacccgccga tgcggatata acacgcgctt gtcgttgtag
     7381 accaggtcga gatagtgatt gaccttgtta cacaggtatc cccgcgtgac cggatgagcc
     7441 ggatcacccg ccaccttgat tgcgcgtccc gaggcattgt cgactgtcac cagcatcgaa
     7501 cagccgtcag ggcagtcatg cgggcaagcc acgcgcacgg tgcgcacttt attcgaaatc
     7561 ccggcttcgt caattacacc catgcgcgtc tcgctccaga catcattgtg cgatattgta
     7621 aaccccggcc actattgccg caccattacc aacgctactc gattacaacc gccgattatt
     7681 caatgaggtc gaaatgcgat acgttcgcac gtgttcaaac gttgttcttg ccgctgcact
     7741 ttcgctctgt gccaatgtaa acgcggggct ggtgaaactc gaaatcgcca aacgcgaact
     7801 tgtggcggat ggcgcgggct ttggatctgc cggcccatac gaaaaactga ccggacgcgc
     7861 ctggttcgaa atcgatccgg cattggaaag gaacaaagcg gtgtttgaca tcgaccacgc
     7921 gcctctgaac gcgagcggca aggtggaatt ttccgcggac atggtcatcc tgaaacccgt
     7981 cgacatggaa aaatcctccc gcacgttgtt tttcgaagtc aacaatcgcg gtcgtaagat
     8041 ctccttcggc cgcatgcagg acacggccgc ggacgcgaac atgaacgacc cgatggcggc
     8101 gcgcgatttt ggcaatggtt ttctgatgaa acggggctat gtcgtggcct gggtgggctg
     8161 gggtgccgac atcgcgcccg gcgacagtcg cctgacggtg acttttccga tcagccagga
     8221 aaacggccgt cccgttaccg agcgtatcct caccgagttt tcagaccgca atttcaatgg
     8281 cggaaaacca acatccctgc cgttgtccgg aagcacggca ttcaaatcct atgccgccgt
     8341 ttcgagcgac aaaaaactgg cgcacgcgga actttacgcg ctcccctccg attcgctgaa
     8401 atcgagtacg gcagacattc cacgtggcga agcgatccct gacgatcaat gggcgctggc
     8461 cgactgcccc gatggctggc ctggccagcc aagttcgcaa cacatttgcc tgaaagaagg
     8521 cttccgcaat gaccgaaatt accatttgat ctaccgggcg accggctcac cagtcatggg
     8581 cctgggctat ctcacgacgc gggacttcgt ttctttcctg aaaaacgacg agcgggacaa
     8641 atcgggaaat ccgaatccag tattcggcat caaaaccacg ctttgccagg gcatttcgtc
     8701 ttctgggatg tatttgcgcg attacctcta tcagggattc aacgtcgatg agcaggagcg
     8761 gcaagtgtgc gaaggcatgc acattcatgt cgccggcgtg caaaagctat atctgaatta
     8821 ccgcttcgct cagccgaatc cctttacaca gcagcatcgc gaacgctacg tgccggacac
     8881 caattttccc cgccagtacg cggtccgcat caatccattc acacgcgtac cggatggcat
     8941 tctgaagcgg ccaaaaacag atccgaaaat cattcatacg gacacctcca atgagtactg
     9001 gcaattccgt gcttcactga ccggatccag cgaaggcggt acgatggact tcaacgaatc
     9061 gtccaaggtc aggcgatatc tgctggcggg actgcagcac ggggggttca aatcagataa
     9121 gccgaatcac ggcctggccg acaagcaatg cgagcaactc accaacacaa cacacccaag
     9181 agtcctacta cacactctaa tcatcgttct ggatgagtgg atcataaaca acgtcacccc
     9241 acctaaaact cgcgtgccaa aaatcagtga taaaacgctg gtcacgccgg ccgaactcaa
     9301 tctgcccaaa attcccggat tcgcctatga aggcctgtat aacggttcgg gagaccgcga
     9361 ttttggaccg cgtgtaacgg gcaattcagg ggtcatcgat cttttgattc ccaagacgct
     9421 tgcgccgcac aaaatacttg ttccccaggt cgatgccatt ggcaacgata tcgc
//
LOCUS       DATMZQ010000533         2797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5129471, whole genome shotgun sequence.
ACCESSION   DATMZQ010000533 DATMZQ010000000
VERSION     DATMZQ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5129471"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VN496_17090"
     CDS             <1..605
                     /locus_tag="VN496_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXF18727.1"
                     /translation="GDVLHGEEYFPELVPAGTGNAQVFSSSPSGGSESMRLMYLLAIT
                     AASRSIHLSTPYFVPDALATQELIDAANRGVHIQIITVGSHTDAAVVRSASRARWGPL
                     LEAGIEIYEYQPTLYHSKAMIVDGLWITVGSTNFDPRSFSLNDEANLTYYDAEFARQQ
                     VEIFQQDLAKSRRMTLQQWQQRPRSERFLDWAASIFGSQL"
     gene            672..2321
                     /locus_tag="VN496_17095"
     CDS             672..2321
                     /locus_tag="VN496_17095"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18728.1"
                     /translation="MADTVARGFPATVLAWLDRNIFELGREMRLSYLPPLMVYVAAGI
                     SGLTAVVGTFYVKERLGLSAEFLAALGFWMMLPWALKMPLGHLVDLIWRWKGWLVYLG
                     AGLIALSILIMIGLLGYTDAMRAFAPVETWYVAASLLAPIGYVVQDVVADAMTVEAVP
                     RYYDDGRKIEAPEIKAMHTTMQTLGRVAIIGGSVAVAILNIFLLRGVGEMPEAQKNEA
                     YLFVYELALVIPVVSVLGVVLASWLRRKAVARLLAEGHTRAECHAILDRHIESPPVNW
                     WILGGGLAFAIVSIGVGLGQIPGAQEIIFCLSMAIVVFMMWRLTRELEPDARHYLVGT
                     AIVVFAFRATPLPGAGATWWVIDALGFDQQFLAMLNLIASGLTLLSLFIFRRFMAEHS
                     IAYVVGVLTIIGTLLSLPNLGMYYGLHIWTAAHTNGVVDARFIALVDTALESPLGQIA
                     MIPMLAWIANSAPEKLKATFFAVMASFTNLALSASQLGTEYLNRIFVITREVKDPATG
                     QITTPADYSQLGVLLITVIVIGILLPFAAILFARFSRFRNA"
     gene            complement(2540..>2797)
                     /locus_tag="VN496_17100"
     CDS             complement(2540..>2797)
                     /locus_tag="VN496_17100"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXF18729.1"
                     /translation="PGLAGLIGSRERREYTVVGPTLNLAARVQSLTREHHQDILLTEA
                     VRAELDPHFKLSAMPALPVKGIAEPVVTYAVLEGRQEALKT"
BASE COUNT          507 a          808 c          843 g          639 t
ORIGIN      
        1 gcggcgacgt tttgcacggg gaggaatatt tcccagaact cgtgcccgcc ggcaccggca
       61 atgcacaagt gttcagcagt tctccctcgg gcggaagcga gagcatgcgc ttgatgtact
      121 tgctcgccat tacggcggcg agccgctcca ttcacttgtc gacgccgtat ttcgtgcctg
      181 atgcactcgc aacgcaagag ttgatcgatg ccgcgaatcg cggcgtgcac atccagatca
      241 ttactgttgg ttcgcacacc gatgccgctg tggtgcgcag tgcgtcgcgt gctcgctggg
      301 ggccgttgct ggaggccggc attgaaatct atgagtatca gccgacgttg taccattcca
      361 aggcgatgat cgttgatggt ttgtggatta ccgtcggctc aaccaatttc gatccgcgct
      421 ccttcagtct gaacgacgaa gccaacctga cctattacga cgcagagttc gcgcggcagc
      481 aggttgaaat cttccagcag gacctggcca aatcgcgcag aatgactttg caacaatggc
      541 aacagcggcc gcgttcagaa cggttcctgg attgggctgc atcgattttt ggctcgcagt
      601 tatagcgctc ggcgcgcgct gtcggtgagg cacaatggaa tacccggaag gtttcgctgt
      661 ccctcacgta catggctgac acagtcgccc gcggtttccc cgccactgtt ctggcctggt
      721 tagatcgcaa catcttcgag cttggccgcg aaatgcggct gtcctatctc ccgccgctga
      781 tggtttacgt cgcggccggc atctccggcc tgactgccgt ggtcggaacc ttctatgtga
      841 aggaacgcct cggattgagc gcggagtttc tcgcggctct cggtttctgg atgatgctgc
      901 cctgggcgct gaaaatgccg ctgggtcatc tggtggactt gatctggcgt tggaaaggct
      961 ggctggttta tctgggcgcc ggactgatcg cgctcagcat cctgatcatg attggcctgc
     1021 tcggctatac cgatgcaatg cgtgcgttcg cgcccgttga gacctggtac gtggccgcgt
     1081 cgttgctggc gccgattggt tacgtggtac aggacgtggt cgccgatgcc atgacagtag
     1141 aagcagtgcc gcgctattac gatgacggcc gcaagatcga agcgcccgag atcaaagcga
     1201 tgcataccac catgcagaca ctcgggcgcg tggcgatcat cggcggcagc gtggccgtgg
     1261 ccattctaaa tattttcctg ttgcgcggcg tgggcgaaat gccggaagcg cagaagaacg
     1321 aagcctatct gttcgtctat gagctggcgc tggtcattcc agtcgtgtcg gtgctgggcg
     1381 tagtgctggc ctcttggctg cggcgcaagg cagtcgcgcg cctgcttgcc gaaggccaca
     1441 cgcgtgcgga atgccacgcg attctggatc gccacatcga atcgccgccg gtcaactggt
     1501 ggatcctggg cggagggctg gcgtttgcca tcgtctccat aggagtaggc ctcgggcaga
     1561 ttcccggcgc gcaggaaatc atcttttgcc tatcgatggc gattgtcgtt ttcatgatgt
     1621 ggcggcttac ccgcgaacta gagcccgatg cgcgccacta tctggtgggc acggccatag
     1681 tggtgttcgc ctttcgcgct acaccgttgc ccggcgccgg cgccacatgg tgggtcatcg
     1741 acgcattggg gttcgaccag cagttcctgg ccatgctgaa tctgattgcg tcgggcctga
     1801 cgctgctcag cctgttcatt ttccggcgtt tcatggctga gcattcgatt gcgtatgtcg
     1861 tcggcgtgct gaccatcatc ggtacgctgc tgtcattgcc caatctcggc atgtattacg
     1921 gattgcatat ctggacagcg gcgcatacca acggcgtggt cgatgcgcgc ttcatcgcgc
     1981 tggtggacac ggcgctggaa tctccgctgg gacagatcgc gatgattccg atgctggcat
     2041 ggattgccaa ttccgctccg gaaaagctca aggcgacgtt ctttgccgtg atggcatcgt
     2101 tcaccaatct ggcgctatcc gcatcgcaac tcggcaccga atacctgaac cggatttttg
     2161 tgatcacgcg tgaggtcaag gatcccgcta ctggccagat caccacgccg gccgactata
     2221 gccagctcgg tgtgctgttg atcaccgtca tcgtgatcgg catcttgcta ccgttcgcgg
     2281 caatcctgtt tgcgcgtttc tcgcggttca ggaatgcgtg ataagggccg caaaggccgt
     2341 gaatgtcgtg aatatcgtga attcgtgaat gtcgtaaaca cgggaaagag tcgtgacgat
     2401 gaaaccgtga ctgccgtgag tatcgtaact gccgtgaatt cgtggcgcgg ccctatgccg
     2461 tcacgccgac aaagacgatg aggcatttgc gatattcacg acattcacga tattcacgac
     2521 atttacggct tgcccgtcgt cacgtcttta acgcctcttg cctgccctcc agcaccgcat
     2581 aagtcaccac cggttcggca atccccttca ccggtagcgc tggcatcgcc gacagcttga
     2641 aatgcggatc cagttcggcg cgcactgctt ccgtgagcaa aatatcctgg tgatgttcgc
     2701 gcgtgagcga ctgcacgcga gccgcgagat tcagcgttgg accgaccacg gtgtactcgc
     2761 ggcgctcgcg gctgccaatc aggccagcca gccccgg
//
LOCUS       DATMZQ010000534         3347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2374401, whole genome shotgun sequence.
ACCESSION   DATMZQ010000534 DATMZQ010000000
VERSION     DATMZQ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374401"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VN496_17105"
     CDS             complement(<1..583)
                     /locus_tag="VN496_17105"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXF18730.1"
                     /translation="MKKEKRWRTIIRWLRDARISLGLFTFSVAVLVSAIFYVALSIEN
                     LMGDYSAMASSQSVIGALATTRNAVEARVNALRSYVITGNENLLVDYRNAGSDSIASL
                     ANLKGLVSGNAAATKRTEALQRTLATQAAVMERILDAYQHHRVEHVRQLLIEDDTKLR
                     TLDHVRRAISASDMAEHATLSAATARSNATLRSA"
     gene            complement(807..895)
                     /locus_tag="VN496_17110"
     tRNA            complement(807..895)
                     /locus_tag="VN496_17110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(859..861),aa:Leu,seq:taa)
     gene            982..1365
                     /locus_tag="VN496_17115"
                     /pseudo
     CDS             982..1365
                     /locus_tag="VN496_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
     gene            1607..2059
                     /locus_tag="VN496_17120"
     CDS             1607..2059
                     /locus_tag="VN496_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18731.1"
                     /translation="MKLAWKVACLILASATPALGAEDFTFTATGHDGASAWVKIDGQN
                     IGATTSTVSSVGVGPDGATQTSNGVCQSWTPDPMGPYSFETVCNYKNDLGTYATISWC
                     PKDTHKGCSGKLVGVDGAYANKAGTFSVLTKPGADDATDTYSGSGHWN"
     gene            2194..2823
                     /locus_tag="VN496_17125"
     CDS             2194..2823
                     /locus_tag="VN496_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18732.1"
                     /translation="MSVEDRIKHQFPALFITLLSVLIALVFSDLASEAHARMTLWPLN
                     LSTLRTWGQIFAMGTNALGAWVVFAHVGISRLRIPSLADSIIVFLVPIPLLFANSLVG
                     QKDIWPWFYYASGYLLIAFATWLWQVRMASAESELASFARLTHPLGPVLVLYIGIPFF
                     AAAGWADSHRLVPPVVEMLLVMSAIPAPLVFAHLFFRDWRRAIGEAQVG"
     gene            complement(2827..3006)
                     /locus_tag="VN496_17130"
     CDS             complement(2827..3006)
                     /locus_tag="VN496_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18733.1"
                     /translation="MMIVVRFQQGAQQLTVFTCPLPSPEKLHECVTAAFQLFYKEHPG
                     LALLDGVIVKFDKAE"
     gene            complement(3156..>3347)
                     /locus_tag="VN496_17135"
     CDS             complement(3156..>3347)
                     /locus_tag="VN496_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18734.1"
                     /translation="LTGLLGRPQMLSPMRRSILYFALARLLMLSPDLADFPLGCEVFP
                     AKAAHYSQRKVEVKRSRSG"
BASE COUNT          638 a          973 c         1008 g          728 t
ORIGIN      
        1 tggccgaacg caacgtcgcg ttcgatcgcg cggtggcggc gctgagcgtg gcatgctctg
       61 ccatatcgga ggcggaaata gcgcgccgca catgatcaag cgtgcgcaat ttggtgtcgt
      121 cttcgatcag gagctggcgg acatgctcga ccctgtgatg ctggtaggca tccaaaatcc
      181 gttccatgac tgcggcttgc gtcgcgagcg tgcgctgcaa tgcttcggtg cgtttggtcg
      241 cggcggcatt gccgctgacc aggcccttga gattggccag actggcgatg ctgtcggacc
      301 cggcattcct atagtcgacc agcagatttt cattgccggt gatgacatag gagcgcagcg
      361 cattcacccg ggcttcgacg gcgttgcgcg tcgtggccaa cgcgccgatc acggactgtg
      421 agctcgccat cgccgaatag tcgcccatca aattttcgat gctgagggcc acatagaaaa
      481 tcgccgatac caggaccgcg accgagaatg tgaacaggcc caaagagatc cgcgcgtcgc
      541 gcagccagcg aatgatggtc cgccaccttt tttctttttt catgcctgcc ggttcggtgg
      601 tgctagccaa ttgtgccaac cagtaggtgc gccaacgccg tacataaatc gtaaatttgt
      661 tctcacctac gtagtactac gtaggcccaa gggagctttg gcggcaaagg ctggtctgcc
      721 gagcgtagct gtgcgaaccc ttcaccggcc cgtcttcgcg ctaacgcgct tcgtcgtggc
      781 agtctccact gctgcgcagc ggagactggt ggccccggac cgactcgaac ggtcacgtcc
      841 ctttcggaac tacggatttt aagtccggtg cgtctaccag ttccgccacg gggccccggc
      901 gcaaactgtg tggcgtatcg cggcgcgtca atagcccaga ctgtcagggg ccatcgttcc
      961 gctgttcccg gctaggatca gaagatgttc gctcgcgtga aggactgggc gcgccgcgtc
     1021 aagcgggacg tgcatgcgct gtacctcgca agccgcgatc cccgcgtccc ctggtatgca
     1081 aaggtgttgg ccgttgcggt ggccggctat gccctatcac cgattgacct cattcccgat
     1141 ttcattccgg tgcttggata cctcgatgac atcatcatag ttccgctcgg catcatgctg
     1201 gtagttcggc tgatcccgcc cgagatcatg gcagaacacc gcgagcgcgc cgcagcggcg
     1261 caagagcggc ccatcagccg cgttgcggcg gtgttgattg ttgtggtttg gctggcctgc
     1321 gccacgctgc ttggctggtt cgtctatagt tatttccagc aataggattg gcgaggaccg
     1381 agatggggtt ttgaacttgt cgatggcagc gtcgcggcgc gcactattga atgacctcga
     1441 cggccacccg ctctatttcg tcaacctgcc aggcgtgaca cggaaggttc gggcgtatga
     1501 gccaaacatc gctgacagag aggcgtcgtc gacgatatcg ccggtcgttt cgcgcccctt
     1561 gcgcatgcgc ttagtatggc caggaatacg aagggtggag gagtgcgtga aactggcatg
     1621 gaaagtcgcg tgtctcatat tggcgagtgc gactccggcg cttggggccg aggattttac
     1681 cttcacggcc acgggacatg atggcgccag cgcgtgggtc aaaatcgatg gccagaatat
     1741 cggcgcaacc acctcgacgg tttcgagcgt cggagttggt ccggacggcg cgacgcagac
     1801 ctcgaacggg gtctgtcagt cgtggacgcc cgatcccatg ggaccctatt cgttcgagac
     1861 cgtgtgtaac tacaagaacg acctgggcac ctacgcgacg atttcctggt gccctaagga
     1921 cacgcacaaa ggctgttcgg gcaaattggt tggcgtcgac ggtgcctatg cgaacaaggc
     1981 cggcacgttc tccgtgctca ccaaacccgg tgcggatgat gcgaccgaca cctattccgg
     2041 ctcgggtcac tggaactgat tgctgggagt tcgcgtccaa ccgaaggacg gcggtttcga
     2101 tgacatcggc aaggcgtttc tggccttggg gacgaccttt gggcgcgggc ataggtatga
     2161 catctttgcg cccgatttgg tggaggggac gatatgagtg tggaagaccg catcaagcat
     2221 cagtttcctg ctcttttcat cacattgctc agcgtcctga tcgcgttggt attctccgat
     2281 ctggcgtcag aagcccatgc gcgcatgacg ctttggccgc tcaatctcag taccctgcgc
     2341 acttggggtc agatattcgc aatgggaaca aacgcattgg gcgcgtgggt ggtcttcgcc
     2401 catgtcggca tttctcgtct gcggattccg tcgttggccg actccattat tgtgtttctg
     2461 gtcccgatac cgctcctgtt cgccaattcg cttgtcgggc agaaggacat ctggccgtgg
     2521 ttttactatg caagcggcta tctgctgatc gcgtttgcaa cctggctatg gcaggtccga
     2581 atggcgagcg cggaaagcga acttgcatct ttcgcccgtc ttacccatcc gctcgggccc
     2641 gtactggtgc tctatatcgg cattcccttc tttgccgccg caggctgggc cgattcccat
     2701 cgcttggtac cgccagtcgt cgaaatgctg cttgtcatga gcgcaatacc cgccccgtta
     2761 gtcttcgcgc acctgttctt ccgcgactgg cggcgcgcca taggggaggc gcaggtgggt
     2821 taggcatcac tcggctttat cgaatttcac gatcacgccg tcgagcaatg ctagtccggg
     2881 gtgttccttg tagaagagct ggaacgccgc ggtgacgcac tcatggagct tttcgggcga
     2941 tgggagcggg caggtaaaga cggttagttg ctgcgcgccc tgctggaacc tgacaacaat
     3001 catcatggaa aacgaactcg cggctgatac acgcaccaac cataggctcg cgagaaacca
     3061 accagcaact tggaccaaaa acaaactgga ccagtgccgg tcatcgaaga gcgctgtaac
     3121 aacccgttct tttcattgac acttttccca ggcactcaac cagagcgcga gcgtttgact
     3181 tcgacttttc gctgactata gtgcgcggcc ttcgcgggga acacctcgca acccagcgga
     3241 aaatccgcca agtccggact aagcatgagc aagcgcgcca acgcgaaata caagattgat
     3301 cgccgcatgg gcgatagcat ctggggccgg cccaagagcc cggtcaa
//
LOCUS       DATMZQ010000535         5156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1656564, whole genome shotgun sequence.
ACCESSION   DATMZQ010000535 DATMZQ010000000
VERSION     DATMZQ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656564"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VN496_17140"
     CDS             complement(<1..446)
                     /locus_tag="VN496_17140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXF18735.1"
                     /translation="MGRLLLCLTLAITVLLPGCATVKNPVTGKNEHTVMDERSEMAEG
                     AKAHPQILAEFGEYKDPELQAYVNEVGQRLAKASHRPELDWHFTVLDTPDVNAFALPG
                     GYVYVTRGIMAYMHSEADLAGVIGHEIGHVTARHGSQRATREQTAG"
     gene            complement(553..789)
                     /locus_tag="VN496_17145"
     CDS             complement(553..789)
                     /locus_tag="VN496_17145"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HXF18736.1"
                     /translation="MAWKGLDLRTAKRIVVAVIGGTITLVGVAMIVLPGPAFIVIPLG
                     LSILATEFIWAKRWLQKIRQMASRIAAKKPPPRP"
     gene            complement(925..1713)
                     /locus_tag="VN496_17150"
     CDS             complement(925..1713)
                     /locus_tag="VN496_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXF18737.1"
                     /translation="MSDWNWTKVAAPLMVGFGFLVLWHMLVVINNIPPYIVPGPLLVL
                     QTLAQEWQALFASLIITLQVTASALLLAVVLGSLLSVLFVQSRLLESALLPYAVVLQV
                     TPLVAIAPLIIIWVNEPFEALVICAAVASIFPVIANTTVGLRSVDTGLLDLFRLYKAS
                     RAQVLWRLRIPSAAPFFFAGLRVATALALIGAVVAEFVAGTGGTRSGLAYQILQAGLQ
                     LRIPRLFAALFLITATGIILFLTVSALSRLALRRWHESELPHEP"
     gene            complement(1710..2480)
                     /locus_tag="VN496_17155"
     CDS             complement(1710..2480)
                     /locus_tag="VN496_17155"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXF18738.1"
                     /translation="MSSLPAIAVDIRGASKIYPNGTRALEPVSLSLCEGEFVTIIGPS
                     GCGKSTLLRLIAGLAMPTQGEIVLSQRFTSSALAYVFQSPTLMPWATVQANVRLPLDL
                     AGVAREQADDTARRALERVSLQDFALSFPRELSGGMQMRASIARALVTGARLLLMDEP
                     FGALDEINRNRLDTEFAELSKREKLTVLFVTHSIYEAVFLSSRVLVMSSRPGTISGEI
                     SIDLPRPRSDTFRGTPEFAAYGAKLSKMLAGAMQEDAT"
     gene            complement(2544..3626)
                     /locus_tag="VN496_17160"
     CDS             complement(2544..3626)
                     /locus_tag="VN496_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXF18739.1"
                     /translation="MSKIVVRPAWLVSNEAGEELDQQLFPVLQAIHDAGKLTVAASTA
                     GLSYRHAWNLIGRWSDFFGSPLVTLERGRGAALTPLGEKLLWAEQRINARLAPQLESL
                     ASELNHEISKLITTARSTLRIHASHGFAVAKLPELLGAHSQIQLDLRYLGAQESLASV
                     ARGACEFGGFHVPEGRLGVQALAQYSKWLLPDKQKLIHLVTRTQGLFLARQNPKNIHG
                     FADLGRPGLVFINRQRGSGTRLLIDQLLEEAGVDHQRLDGYQTEEFTHAAIAAYVASG
                     MADAGFGVEPAARQFALDFLPIAKERYFLICSNESLQLPESRQLIQLLRGPAFLKLIA
                     GLAGYSAPRAGEVESVADAFASVEHR"
     gene            3818..4297
                     /locus_tag="VN496_17165"
     CDS             3818..4297
                     /locus_tag="VN496_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXF18740.1"
                     /translation="MSHEAVIARIVEKHRHEPGALLPILHDIQDALRYVPREAVGLIA
                     HGLNLSRAEVHGVLSFYHHFRTAPPGRHTIYVCRAESCQSMGAGALIEHVKDKLGVDL
                     HDTTRDGAFTLEPIYCLGNCACSPAMMIDDQVYGRVTPDRFDEAVRMVKNGLQEVAA"
     gene            4294..>5156
                     /locus_tag="VN496_17170"
     CDS             4294..>5156
                     /locus_tag="VN496_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HXF18741.1"
                     /translation="MSIRVYVPRDSSALSMGAEAVASAVASEAARHNADIAIVRNGSR
                     GLYWLEPMVEVETSLGRIAYGPVTTKDVASLFEADFLHGGRHALLRGPVDEIPYLRKQ
                     ERLTFARVGITDPLSLEDYAAHGGWQGLRAALAMDGASAVKQVTDSGLRGRGGAAFPA
                     GIKWKTVLDTPSHQKYVVCNADEGDSGTYSDRMIMEGDPYVLIEGMAIAGIAVGATHG
                     YIYVRSEYPHACQILADAITLAREQSYLGANILGSDKAFELEVRKGAGAYICGEETSL
                     LESLEGKRGMVR"
BASE COUNT         1114 a         1477 c         1573 g          992 t
ORIGIN      
        1 atccccgccg tctgctctcg agtagcgcgt tgggaaccgt gccgcgcggt gacgtggccg
       61 atctcgtggc caatgactcc tgccaggtcg gcttcactat gcatgtaggc catgatgccc
      121 cgcgtgacat agacgtagcc cccgggcaac gcgaatgcat tgacgtccgg ggtatcgagc
      181 acggtaaagt gccaatcaag ctccgggcga tgcgatgcct tggccaaacg ctggccgact
      241 tcgttgacgt aggcttgcag ctcgggatcc ttgtactcgc caaattcggc aagaatctgc
      301 ggatgcgctt tcgccccttc cgccatttcg ctgcgttcat ccatgacagt gtgctcattc
      361 ttgcctgtca ccggattttt gacggtggca caaccgggta gtagaaccgt gatggcgagc
      421 gtaagacaaa gcagcagccg gcccatggca ttctcctgac aaaaccgttg gacaggttcg
      481 gagttcaggc gttgcagaac gttcggcacg gtcactggca acatcggcag gatctgcgca
      541 gtcgcgatcg gatcaggggc gaggtggagg ttttttagcc gcaatacggg aagccatttg
      601 acggatcttt tgcagccagc gtttggccca gatgaattcg gtcgccagta tggaaaggcc
      661 gagcggaata acgatgaatg caggtcccgg caatacaatc atggcgacgc cgacaagcgt
      721 gatggtccca ccgatgaccg ctacgacaat ccgctttgct gtgcgaagat ctaacccctt
      781 ccaggccata cgaagctctt acctggaccc gtcccggatc gcatgaacca agtcagccct
      841 ggcagcgttt gagcgggcca acccaagtcc acgctgacgt atcgtggttc atgccgtgcg
      901 ccgtagcaag acccaggctt tgtttcacgg ctcatgcggc aactcgcttt catgccagcg
      961 ccgcagagct agtcgggaca acgcgctcac ggtgaggaaa agtatgatgc ccgtcgcggt
     1021 gatcagaaag agcgcggcga acaagcgcgg gatcctgagt tgcaggccgg cctgcaaaat
     1081 ctggtaagcc agacccgatc tggtgccgcc ggtgccagcg acaaattcgg caacaacggc
     1141 accgatcagt gccaatgctg tagccacccg tagcccggcg aaaaagaaag gtgctgcact
     1201 tgggatgcgc aagcgccaca gaacttgcgc acgcgatgcc ttatagagac ggaacaagtc
     1261 gagcagtccg gtatccacgc tgcgcaatcc cacggtggta ttggcgatta ccgggaaaat
     1321 ggacgcgact gccgcacaaa tgaccaaagc ttcaaacggc tcgttcaccc agatgatgat
     1381 gagcggcgcg atggcgacca acggagtgac ttgcaagacg accgcatagg gcaacaaggc
     1441 ggattcgagc agccggcttt gcacgaacag cacggaaagc aaacttccca ggacgaccgc
     1501 cagcaacaag gccgaagcgg tcacctgcaa ggtaataatc agcgaagcaa aaagcgcttg
     1561 ccactcctgt gccagggtct gcaggaccag cagcggtcca ggcacgatat acggcgggat
     1621 gttgttgatg acgaccagca tgtgccacaa caccaggaag ccgaatccca ccatcagcgg
     1681 agcggccact ttggtccaat tccaatcgct catgtcgcgt cttcctgcat ggcaccggcc
     1741 agcattttgg atagcttcgc gccgtacgcc gcaaattcag gagttccgcg aaaggtatca
     1801 gagcgcggcc gcggcagatc gatgctgatt tccccgctga ttgttccggg gcgcgaagac
     1861 attacaagaa cccgcgacga caggaatacc gcttcataga tgctgtgcgt gacgaacagc
     1921 accgtgagct tttcacgctt gctcagttcg gcaaactcag tgtccaggcg gttgcggttt
     1981 atttcgtcca acgcgccgaa cggttcgtcc ataagcagca gccgcgcacc ggtcacgagt
     2041 gcccgcgcaa tggaagctcg catttgcatg ccaccggaaa gttcacgtgg aaacgatagc
     2101 gcaaagtcct gaaggctgac tcgctccagg gcgcgccgtg ccgtgtcgtc ggcctgttcg
     2161 cgtgcgacac cggccaagtc caggggcaac cggacattgg cctggaccgt tgcccacggc
     2221 atcaaagttg gagactggaa tacgtaggcc aacgcggagg aagtaaaacg ctgcgacagg
     2281 acgatctcgc cttgcgttgg catagccaat ccggcaatta atcgcagcag cgtacttttg
     2341 ccgcagccgg aaggaccgat gatggtgacg aattcgcctt cacacaggga tagactgact
     2401 ggttcgagcg cacgtgtgcc attgggatag atttttgaag cgccgcgaat gtcgactgcg
     2461 atagcgggaa gggagctcac ggcgcgattc tagcagcgcg gctttcgggc aagtccggaa
     2521 tccgggttcc ggcccaataa cgcttatcga tgctccaccg aagcaaaagc gtcggcaacc
     2581 gactccactt cgcccgcccg cggcgcgcta taaccggcca ggccagcaat gagcttgaga
     2641 aaagccgggc cgcgcaatag ctggattaat tgcctggatt cgggcagctg cagcgattcg
     2701 ttgctgcaga tgaggaaata gcgctccttc gcgatgggca ggaagtcgag cgcgaactgg
     2761 cgtgcggctg gttccacgcc gaacccggca tcagccatgc cgctggcaac gtaggcggcg
     2821 atggcggcat gcgtaaattc ttctgtctga tagccatcga gccgttgatg gtctactccc
     2881 gcctcttcca gcaactgatc gatcagcaac cgggtccctg aaccacgctg cctgttgatg
     2941 aacaccaggc cgggacgccc gaggtcggca aagccgtgga tatttttcgg gttttgccgg
     3001 gccagaaaca agccttgggt tcgcgtcacg agatgaatca gcttctgctt gtcgggaagc
     3061 agccatttcg aatattgagc aagcgcctgc acgccgagcc tgccctccgg aacatgaaaa
     3121 ccaccgaatt cacacgctcc gcgcgccacg gacgccagcg attcctgcgc gccgagataa
     3181 cgtagatcaa gctggatctg cgaatgcgcg cccaacaatt ccggcagttt ggcgaccgca
     3241 aagccatgac ttgcatgaat gcgcagagtt gagcgggccg tcgtaatcag cttgctgatc
     3301 tcgtgattga gttcggaagc gaggctctcc agttgcggag ccaggcgagc attgatgcgc
     3361 tgctcggccc aaagcaattt ttcgcccagc ggggtgagtg ccgcgccgcg cccacgttcg
     3421 agcgtaacca gcgggctgcc gaagaaatct gaccagcggc cgatgagatt ccaggcgtgc
     3481 cggtatgaca ggccggcggt cgaagccgcg acagtgagtt ttcccgcgtc gtgaattgcc
     3541 tgcagcaccg gaaaaagctg ttgatcgagt tcttccccgg cttcgttgct gaccaaccag
     3601 gcggggcgaa cgacaatctt gctcaccgtg agccactccg ggacacgcag gttgtagggc
     3661 ggcttatata tgcacaatcg ttcatattgt gtgatgatgg ctcaagcgct agctttcact
     3721 ctcaaaagca tagcatcgcc tcgcaaatat gcaatttctt gcataacttg aggtcgaatg
     3781 tcagatggat tgcgcatcga catgtgaagg aggatttgtg tctcacgaag cagtaatagc
     3841 ccgcatagtg gaaaagcaca ggcacgagcc tggtgcgttg ctgccgatcc ttcacgacat
     3901 tcaggacgcc ctgcgttatg tgccacgcga agcggtgggt ctgatcgcgc acggattgaa
     3961 tctgtcgaga gcggaggtcc acggcgtgct gagtttctat catcactttc ggacggcgcc
     4021 gccgggacgg catacgattt acgtgtgccg ggccgaatct tgccaatcaa tgggcgctgg
     4081 tgcactgatc gagcatgtca aggacaaatt gggcgtcgat cttcacgaca caacgcgcga
     4141 tggtgcgttt acgctcgagc cgatctattg cctcggcaac tgcgcgtgtt cccccgcgat
     4201 gatgattgac gaccaggtgt acgggagggt gacgcccgac cgcttcgatg aggcagtacg
     4261 catggtgaaa aacggtctcc aagaggtagc ggcgtgagca ttcgggttta tgtcccgcgc
     4321 gattcgtcgg cgctgtcgat gggtgccgaa gcggtcgcct ccgccgtggc atccgaagca
     4381 gctcgccaca atgcggacat cgctatcgtg cgcaacggtt ctcgcgggct gtattggctg
     4441 gagccgatgg ttgaagtaga gacttcgctc ggccgcattg cctacggccc ggtgacgacc
     4501 aaggatgttg cttcgctgtt cgaggccgat ttcctgcatg gcggtcgcca tgcgctcttg
     4561 cgcggcccgg tcgacgaaat tccctatctg cggaagcagg aacgtctaac cttcgccagg
     4621 gtcgggatca ctgatccgtt gtctctggaa gattacgcgg cgcatggcgg gtggcaaggg
     4681 ttgcgtgcag cactggcgat ggacggcgct tcggcagtca agcaggtgac cgattcgggg
     4741 ttgcgcggac gcggcggcgc ggcttttccc gccggcataa agtggaagac ggtgctggat
     4801 acgccctcgc accagaaata cgtggtgtgc aacgcggacg agggcgactc cgggacctac
     4861 tccgatcgaa tgatcatgga aggcgacccg tacgtgctga tcgaaggcat ggcaatcgcc
     4921 ggcattgcgg tcggtgcgac gcatgggtac atctatgtgc gttcggaata cccgcacgcc
     4981 tgccagatcc tggccgacgc cattacgctc gcgagggagc aaagctatct tggcgccaac
     5041 attctgggca gcgacaaggc cttcgagctg gaagtacgca aaggcgccgg cgcttacatt
     5101 tgcggcgaag aaacatccct gctggaaagc cttgagggca agcgcggcat ggtgcg
//
LOCUS       DATMZQ010000536         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2389935, whole genome shotgun sequence.
ACCESSION   DATMZQ010000536 DATMZQ010000000
VERSION     DATMZQ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389935"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VN496_17175"
     CDS             complement(<1..182)
                     /locus_tag="VN496_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18742.1"
                     /translation="MRQSEPPEPLRDGLWGSRLTVASHADASQVDDRSTRAYASRERA
                     NDVRVFRAARRHSRLVR"
     gene            complement(313..930)
                     /locus_tag="VN496_17180"
     CDS             complement(313..930)
                     /locus_tag="VN496_17180"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HXF18743.1"
                     /translation="MTIRLHRGDLPDLARYQTGSVAIDTETMGLDPRRDRLCVVQLSP
                     GDGSADVVQMPAGRPSAPNLTKLLADTNTLKIFHFGRFDLGVLYQSLGVMAAPVYCTK
                     IASRLARTYTDKHGLKDLVRELLGVDLSKQQQLSDWGSAELSDAQVAYAASDVLHLHA
                     LKAKLDGMLAREGRTALAEACFRFLPDRVRLDLAGWAADDIFSHS"
     gene            complement(1084..>2212)
                     /locus_tag="VN496_17185"
     CDS             complement(1084..>2212)
                     /locus_tag="VN496_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18744.1"
                     /translation="RSDPRHDTINRAAANARNESILLNIIRASHSVPLNFVAFSRVSG
                     SQSAQANIGLPAFGLGPVPLVSSVQKQTVFGSSVFNANTNVNNSFDIGILESKEFYSG
                     LLTPVDLATVNYFGRQGYSRQLLFWLFVESVRETYNGKTYEYRNDPNTAFSCDPDFPR
                     RTRCFRDVINDALANGVTTQIETVSRITNQGRTISTVYGRICQDEVLARRAKEYAGIS
                     GYVSISPRCSEPWKRKLPGIVQASGGRQGGSSETEVEGSPDTLTFELVNHYPIKYEIV
                     SRSTFGIYQYLGGVLRNRHDMIMLRGRRDRNEPDWIPLLEIRSDSSGGCFVDLEFDGH
                     YYCVPKEGAETTKAIFSLLAQLIALRTPGDLAITPAVRVTQ"
BASE COUNT          448 a          668 c          667 g          429 t
ORIGIN      
        1 cgcaccaatc ggctgtgacg ccgggccgca cggaacacgc gcacatcatt ggcgcgctcg
       61 cgcgacgcat aggcgcgcgt ggagcgatcg tcgacctggg aagcgtcggc gtgcgatgca
      121 acggtcaagc ggctacccca caacccgtcc cgtagcggtt cgggcggctc agactgcctc
      181 acaattctta gcaaatccga cggcaccatc gccaacaaat gtggctccgc cgcggcatat
      241 ccccttgaat tcccttgcgg ctccctgcga ccgcgatccg gcctcgttca ccatgcaaga
      301 ctgcgaaaac actcaggaat gcgaaaagat atcgtcggcg gcccagcctg cgagatcaag
      361 ccgcacccga tcgggcagga accggaagca ggcctccgcc agggcggtgc ggccctcgcg
      421 cgccagcata ccgtcgagct tggccttgag cgcgtgcaga tgcaggacgt ctgaagccgc
      481 ataggcgacc tgcgcgtccg agagctcagc cgagccccag tcggagagct gctgctgctt
      541 cgacagatcg acaccgagca actccctcac caggtctttc aagccgtgtt tgtcggtgta
      601 ggtgcgcgcc agccgcgacg cgatcttggt gcaatagact ggtgccgcca tcacgcccag
      661 cgactgatag agcacgccga gatcgaagcg tccgaagtga aagatcttca gcgtgttggt
      721 atcggcaaga agcttcgtca ggttgggtgc gctcggccgc ccggccggca tctgcaccac
      781 atcggcggag ccgtcgccgg gagaaagctg caccacgcaa agccgatcgc ggcgcgggtc
      841 aagccccatg gtttcggtgt cgatcgcgac tgagccggtc tgatagcggg caagatcggg
      901 caggtcgcct cgatgcaggc ggatggtcat tgcggctccc gatccggtga tgtggtgccc
      961 aggagaggga caaggacagt cgagttcatt caagcacctc ccgcgtggcg cgggcacata
     1021 tcgcaccatc gaggcgcaaa gggaatagtc ggccgccgcc cgcttcctgc gcagaaaatt
     1081 cgattactgc gtcacccgca cggccggcgt gatcgccaag tcgccgggcg tccgcagcgc
     1141 gatgagttgc gccaagaggc tgaaaatggc cttggtggtt tcggcgcctt ccttgggcac
     1201 gcaatagtag tgtccgtcga actccaggtc cacaaagcag cctcccgatg aatcgctcct
     1261 gatttcgagc aggggaatcc aatcgggctc gtttcgatcc ctgcgaccgc gcaacatgat
     1321 catatcgtgt cggttgcgca ggaccccgcc gaggtactgg tagattccaa aggtcgagcg
     1381 gctgacgatc tcgtatttga ttggataatg gttgacgagc tcgaaggtga gcgtgtcggg
     1441 gctgccctcg acttctgttt cgctgctgcc tccttgcctt cccccggagg cttgcacgat
     1501 tcccggcaac ttacgtttcc aaggctcgct gcaacgcgga ctgatggaaa cataaccaga
     1561 gattcccgcg tactccttcg ccctgcgtgc cagaacttca tcctggcata ttcgaccata
     1621 caccgtggat attgttctgc cttggttggt tatccttgag actgtctcga tctgcgtggt
     1681 cactccattg gccaacgcgt cattgatcac gtctctgaag caccgtgtcc tgcggggaaa
     1741 gtccgggtcg cacgaaaagg ccgtgttggg gtcgtttcgg tattcgtaag tcttgccgtt
     1801 gtacgtctcg cggaccgatt caacgaacag ccagaacagc aattggcgcg agtaaccctg
     1861 acggccgaag taattgactg ttgccagatc caccggcgtc aacagaccgc tgtagaattc
     1921 cttggactcg agaatgccga tgtcgaacga gttgttcacg ttcgtgttgg cattgaacac
     1981 cgaagaccca aacacggtct gtttttgcac gcttgagaca agcggcactg ggccgagccc
     2041 gaaggcggga agcccaatat tggcttgcgc gctctgggac ccagacaccc gcgaaaaagc
     2101 aacaaaattg agcggtacac tatggctcgc tcgaataatg ttcagcagaa ttgactcgtt
     2161 gcgagcgttc gcagcggcgc gattgatggt gtcgtgacgt ggatcggaac ga
//
LOCUS       DATMZQ010000537         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2403612, whole genome shotgun sequence.
ACCESSION   DATMZQ010000537 DATMZQ010000000
VERSION     DATMZQ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403612"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..683
                     /locus_tag="VN496_17190"
     CDS             195..683
                     /locus_tag="VN496_17190"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXF18745.1"
                     /translation="MAILPSERTEYDDWEHHMFNRIRSKPRFSRRVSVAMNNRLVGTT
                     SLLGDDVYDAAGKFVGEIEEIILDTRTGGVRCAVLALGGFLGIGRKRFAVPWNALTPD
                     TDYQLCVVDLAPTQLRAVPVPNDDPWLQQTDPTWDRENAYMLRPRALLGVTLPRNHFL
                     RL"
     gene            1227..2414
                     /locus_tag="VN496_17195"
     CDS             1227..2414
                     /locus_tag="VN496_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXF18746.1"
                     /translation="MASMVAVLVAGCGGGSAQPDSSSDEAALVAQGKQIFRFDTFGDE
                     SQWSDTLRMHEVIAAAVDPTTALSVGLKVDAEALPPAVVQGIQNGSVDLKSPDTTIAL
                     LKLNAVVGVQGKVETVGGKDTLTRVGITCALCHSTVDDSFAPGIGKRLDGWPNRDLNP
                     GAIIALSPALSAATKLVYNSWGKGKYDPRFNQDGMNGPQVIPPAYGLSGIHRITSTGD
                     GEDIAYWNRYVGVTQMGGHGSFSDPRTGVNITNGSDDLITSKLPALQAYQLSLNAPPA
                     PAGSFDTVAAVRGQRVFNGAGTCSTCHSGIEMTDANSRLHPVSDVVSEPEPNGAPSYA
                     SRSATKQYRTAPLAGIWQHAPYFHNGSAATLEAVVQTYNTKKSLGLNNADMSDLVEYL
                     KSL"
     gene            2577..>3010
                     /locus_tag="VN496_17200"
     CDS             2577..>3010
                     /locus_tag="VN496_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HXF18747.1"
                     /translation="MRIDPLVITATRIEERAFDLPVAIDSIGAARIQQGQLQINLSES
                     LSRVPGLVVQNRWNYAQDLQVSSRGFGARASFGVRGIRLYQDGIPATMPDGQGQTGSF
                     SLASAQRIEILRGPFSTLYGNAAGGVVSIFSEDGPPTPTAQA"
BASE COUNT          627 a          857 c          935 g          591 t
ORIGIN      
        1 ggcacatccc ctgtcgggat gtgccaatgg atcggtcgat ctgtcactac ggagatccac
       61 catgactaag cctcccgcgc gcgtacccac gtcgtccatc ctgctcaccc tcctctcgct
      121 aggaatcatg ccgaggccat cgctgcgtgt cgcttgttgg gaagatggcc agtatgaatc
      181 ggtctgaggc aagcgtggca atactgccgt ccgagcgcac ggaatacgac gattgggaac
      241 atcacatgtt caacaggatc agaagtaagc cacgattttc cagaagagtc agcgtcgcga
      301 tgaacaatcg cctcgtcggc acaacgagtc ttctcggcga cgatgtctac gatgcggcag
      361 gcaagtttgt aggtgaaatt gaggaaatca tactcgacac gcgtaccggt ggagtgcgat
      421 gtgccgtgct ggcgcttgga ggttttctgg gaattggacg aaagcggttc gccgttccgt
      481 ggaatgcgtt gacgcctgac accgactacc agctgtgtgt cgttgacttg gcgccgacgc
      541 agctcagggc cgtgccggtt ccgaatgacg acccctggct gcaacagacc gacccgacat
      601 gggacaggga gaatgcgtac atgttgcggc cgcgagccct cctaggtgtc actttgccaa
      661 ggaatcactt tctgcggctt tgattttgcc ctcgtcgagc cagcccgctc gcatacgcac
      721 gtcgttatga gaatggagtt tgcccgggcg caaggcgaag tgacttttgc tacgacagtg
      781 actgaaggcg cgccgtagcc ccatgcattt ccaattacgt cgcattgagc aatccatcgc
      841 gatccgacct acgcgtctga ggcaagcagt gccgagatgg aacgcaagct tgcgcggaac
      901 gcggcgacct ttcggttcat ctaacccatg taagtgcgtc tatgcggact tttctaaatg
      961 catcgacccg tcgtccagtc ccggagggct gatcgtgtgg taaagcggcc tccaccctgg
     1021 cgaaagcagc gatctgcgta atgttcagga atggcaaaag cacgtctggg cgccagacga
     1081 gccagaaata tcgacaagga gagagatcgg tgaagacata cctatgcata cctgaacgca
     1141 tgcgactcgt tcgcttaata cggtcacaaa tggattcgcg acatcacgcg tttcttcgca
     1201 agttgcatga gctggccgtg ttaggcatgg caagcatggt ggctgtgctg gtcgcgggat
     1261 gcgggggtgg ttctgcgcag cccgatagtt cgagcgatga ggccgcgcta gttgcacaag
     1321 gcaagcagat tttccgcttc gatactttcg gtgacgagtc gcaatggagc gatacactgc
     1381 gcatgcatga agtgatcgcg gcggcagtcg atcccaccac agcattgtcg gttggactga
     1441 aggtagatgc ggaagcttta ccgccggccg tggtgcaagg aatccagaac ggcagtgtcg
     1501 atctgaagag ccccgacacg acgatagcat tgttgaagct gaatgcagtc gttggtgttc
     1561 agggcaaggt cgaaaccgtc ggtggcaagg acaccctgac ccgcgtggga atcacctgcg
     1621 ccctctgtca ttcgaccgtg gacgattcat tcgcgcctgg tatcggcaag cggctggacg
     1681 gctggcccaa tcgtgatctc aatccagggg cgatcatcgc gctgtcgcct gcgctgagcg
     1741 cggctacaaa gctggtctat aactcctggg gcaaaggcaa gtacgaccct cggttcaacc
     1801 aggatggaat gaatggcccg caagtgatcc cccctgcata cggtctcagc ggcattcacc
     1861 gcatcacatc cacgggggat ggcgaagaca tcgcgtattg gaatcgctat gtaggcgtca
     1921 cccagatggg tggacacgga tcgttctccg acccgcgcac tggtgtcaat atcacgaatg
     1981 gctcggacga cctgatcacg tcgaagctgc cggcattgca ggcgtatcag cttagcctga
     2041 atgcgccccc agctccggca ggtagctttg atacggtcgc agccgtgcgg ggacagcgcg
     2101 tcttcaatgg agcgggcacc tgtagtacct gccacagcgg gatcgagatg acggacgcca
     2161 attcgcggct gcatccagtc agcgacgtgg tgagcgagcc ggagccgaat ggagctccca
     2221 gctacgcatc gcgcagcgcg accaagcagt accgaactgc acctctggcc gggatttggc
     2281 agcatgcgcc ttatttccac aatgggagtg cagcaacgct cgaagcagtg gtgcagacct
     2341 acaacacgaa gaagtccctg ggattgaaca atgccgatat gtccgatctt gtcgagtatc
     2401 tcaagtccct gtagccattg tgcggattga agtacatcga gtatatgaac gcgcgcagca
     2461 gctgggctac gcccatggat agcaccaggc attgtgagcg gggttgcatc cggctacgcg
     2521 cgttcgcttt gttgactgcg ggggcgtggg tcggaaccgc tgcggcgcag gacgtcgtgc
     2581 gcatcgaccc gctggtgatc accgcgacgc ggatcgaaga gcgggcgttc gatctgccgg
     2641 tggcgatcga cagcatcggt gcggcgcgga tccagcaggg acagctgcag atcaacctgt
     2701 ccgaatcgct gtcgcgcgtt ccgggcctgg tcgtgcagaa ccgctggaac tacgcgcaag
     2761 acctgcaggt ttcctcgcgc ggcttcggcg cccgagcgag cttcggcgtg cgcggcatac
     2821 ggctctacca ggatggcatt ccggcgacga tgccggacgg gcagggccag accggaagct
     2881 tcagcctggc ttcggcgcag cgcatcgaaa tcctgcgcgg gccgttctcg accttgtacg
     2941 ggaatgccgc cggcggcgtg gtctcgattt tcagcgaaga cggaccgccc acaccgacag
     3001 cgcaggcgca
//
LOCUS       DATMZQ010000538        16516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_5165879, whole genome shotgun sequence.
ACCESSION   DATMZQ010000538 DATMZQ010000000
VERSION     DATMZQ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5165879"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VN496_17205"
     CDS             complement(<1..1022)
                     /locus_tag="VN496_17205"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HXF18748.1"
                     /translation="MHIHLDAVGGVAGDMFIAAMLDAFPDLRDGMLVAIRAAGLPAEV
                     HVGFAEHQDHALTGLRFRIIEPRTAELLVAAHHHPHTPFADIRSHLHSCALAPAVRDH
                     AIAMFSVLAEAEAKVHGKDIETVSFHELGEWDSIADIIGAAYLIDALGATWSVSTLPL
                     GRGKVLTAHGMLPVPSPATVLLLRDFEFADDGLEGERVTPTGAAILRHLGCTQAPARR
                     PRRLLRTGIGFGTRTFPGISNVLRVLAFDETRESHGGDEVAQVQFEVDDQTPEDLAIA
                     LDRLRAHPSVLDVLQTPAFGKKGRLTMRIQLLAEIPNLESVFDACFNETTTIGLRYQI
                     MQRRKLA"
     gene            complement(1051..2376)
                     /locus_tag="VN496_17210"
     CDS             complement(1051..2376)
                     /locus_tag="VN496_17210"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXF18749.1"
                     /translation="MIGDRIEVNMAGGLDIALPRMVHVRQKFATKKLDSVTKAIVEQF
                     KRPEVRSKVKPGMTIAVGCGSRGINNIAECAKAVVSELKALGAKPFIFPAMGSHGGAT
                     AEGQREVLEGYGITESYVGCPIKSSMEVVELGKLEDGMPVYMDKQASEADAVVIIPRV
                     KPHTNFRAPIESGIVKMLTIGMGKIIGATTLHTYGMDMFGELLPKTAKLILAKKNVLF
                     GVAMVENAADETAIIEIVPSEQIFAREPVLLAKAKELMPRLQFDEIDVLVVEKIGKNI
                     SGSGMDPNITGRNCRFIEWNMKPFVKKIAVLGLTPETHGNACGIGGADVTTMKLYKEL
                     DVAKTYANIITSTYLDGGAIPIIMNTDEDAIRLAVKTVVRVKPQDTRIVRIATTLEIM
                     DIHVSEPMLPIIKANPSQFEIVGSPEPFKFDAKGNLYPMLGKQREHAAA"
     gene            complement(2466..3932)
                     /gene="glgA"
                     /locus_tag="VN496_17215"
     CDS             complement(2466..3932)
                     /gene="glgA"
                     /locus_tag="VN496_17215"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HXF18750.1"
                     /translation="MAAAEKLKVLFATSEVVPLIKTGGLADVSAALPVALNALGVDVR
                     VLVPGYPQVMEALKTKSRVAALPGLPGVPAASLVASKLPSGLPLFVVECPIYDRGGGP
                     YQDSNGTDWPDNDLRFGLFSYVGALLSTPSSPFAAWQPHIVHCNDWQTGLTPVYLNYV
                     QGARARTIMTVHNLAFQGIFPQQSANRLGLPPQAFTIDGVEFYGNLSFLKGGLQFADR
                     ITTVSPSYAREIQSEPLGMGFQGLLSHRANVLTGILNGIDVDAWDPESDPYIERYYNA
                     SRLAAKQDNKRALQLRMGLQDAPLTPLFGSVSRMTGQKGLDVLADIAADLVEFPAQLA
                     VLGSGDPALQTRFQELAADHPGQIAVQIGFDESLSHQIEAGADIFAMPSRFEPCGLNQ
                     MYSQRYGTPPLVHGTGGLLDSVVDCIPATLKNRTATGFVFSPLDAPTMLRNCRRAVGV
                     YAEQRSWRQLQKNGMQRDFSWEARARQYLDLYRALPIG"
     gene            complement(4068..6254)
                     /gene="glgB"
                     /locus_tag="VN496_17220"
     CDS             complement(4068..6254)
                     /gene="glgB"
                     /locus_tag="VN496_17220"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXF18751.1"
                     /translation="MPATAQLPAADDLDALAHARHHDPFSVLGLHRAGSDWSLRVFRP
                     YASSIAVRTQSGFAAMKRVHPLGIFAWQGREPPPHPVTLKVKESAREFETLDPYTFAP
                     VIGADDLYLFNEGRLEQAYRMLGSHVEQREGVRGVRFACWAPNAARVSVVGEFNRWDG
                     RTNPMSSHGLSGVWELFVPGIDVGALYRYEIRNRSTGEVFVKTDPYARAHELRPGNAA
                     QVVANDHVWRDEEWMGKRSRWDWLHAPVNIYEVHAGSWRRHPDGRFYTYAELAQSLIP
                     YVLEMGFTHIELLPVSEHPLDESWGYQTTGYFAATSRFGGVDAFKAFVEACHQAGIGV
                     ILDWVPAHFPTDAFSLARFDGTALYEHEDPRLGFHQDWGTHIFNYGRNEVKSFLLSSA
                     HYWLAECHLDGLRVDAVASMLYLDYSRKAGEWIPNKHGGRENLDAIAFLRELNVMVHR
                     DFPGALTCAEESTAWPMVSRPVYLGGLGFSMKWNMGWMNDTLSYMKNDPVHRRFHHNQ
                     LTFGQIYAYSENFLLPLSHDEVVHGKGSLLSKMPGDAWQKFANVRLLIAYQFASAGKK
                     LNFMGNEFAQGREWRASAELDWGLLGIGWHQGVQSMVRDLCRLYRNEAALHELDFEPE
                     GFRWIDCNDADQSVLCFERRARDGSGVVVALNFTPVPRHGYRVGLPRPGAWREVFNSD
                     SSFYAGSNSGNAGIIQSQPFPWMGHGQSADITLPPLGALILRCDAP"
     gene            6457..7740
                     /gene="glgC"
                     /locus_tag="VN496_17225"
     CDS             6457..7740
                     /gene="glgC"
                     /locus_tag="VN496_17225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXF18752.1"
                     /translation="MIDTDLNVRFVSLLTKNTYALVLAGGRGSRLKNLTDWRAKPAVP
                     FGGKFRIVDFPMSNCVNSGIRRIGVATQYKSHSLIRHIQRGWSFLDGRFEEFVDILPA
                     SQRTAEENWYLGTADAVYQNLDILRAYGPDYILVLAGDHIYKMDYGKMLAYHVAKNAD
                     MTVACLEVPRMEATAFGVMHINENWRVTDFVEKPADPPSIPGRADMALASMGIYVFNA
                     PFLYEQLARDTATPNSSHDFGKDLIPHVVPRYGVYAQRFDDSCVGQGGQPAYWRDVGT
                     VDAYWESNLDLTRVTPDLNMYDKDWPIWTHQEQLPPAKFVFDDDGRRGSAVDSVVSGG
                     CLISGTSIKRSLLFSNVRVHSYGSIDDSVILPDVIVNRHVKLRCTIVDKGCVLPEGLT
                     VGFNPEQDRRRFHVSERGITLITPEMLGQEVLHQR"
     gene            7788..9485
                     /locus_tag="VN496_17230"
     CDS             7788..9485
                     /locus_tag="VN496_17230"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HXF18753.1"
                     /translation="MIAEKKLDLAILWHMHQPDFRDHATGEFAQPWVYLHAIKDYSDM
                     AAHLEAHPGVRVTLNFTPVLLDQLEDYSDQFSTGQFRDPLLRLLAQPHLEQVGPVERN
                     LALAACFKANRQKLIDPYPAYKRLLEFARLAQEQGDAGASYLSAQYLSDLLSWYHLSW
                     MGETVRRQYPVLPALIAKAHGFSHDDRMQLLHIIAHEVRQVIPRYRTLFEAGRIELTT
                     TPEYHPIGPLLLSFESALEAAPGSPLPQSTHYPGGDERARAQTEAAQQSHARRFGRQP
                     RGMWPAEGAVSSDFANLLAEAGFEWIASGEGVLVHTLKEHGDALSERPQYLYRPYRLE
                     KGQNTIVCFFRDDRLSDLIGFEYAKWHGKDAAEHFVAALSDIANGVTDQQRPVVSVIL
                     DGENAWEYYPYNGYYFLDELYGALEGHGEIATTTFTQLLDEKREATPLPALVAGSWVY
                     GNLATWIGSADKNRAWDLLCAAKQSFDAVSHSGRLEGGELKRARRQLADCEASDWCWW
                     FGDYNPAESVMAFDALFRSKLRHLYGLLKLPAPAELNLPVSRGKADSEAVNAMRRAS"
     gene            9570..11549
                     /locus_tag="VN496_17235"
     CDS             9570..11549
                     /locus_tag="VN496_17235"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HXF18754.1"
                     /translation="MNKKVSLLFGVHAHQPAGNFIGVIDDAHARCYKPFLETVHGYPQ
                     FKLSVHFSGWLLDVLLQRFPQDMAKLRDMVGRGQVEMFGGGDTEPVLASIPERDRRSQ
                     LAALSSRLEASLGQRPRGAWLTERVWESSVASALVDSGIRYVTVDDYHFLCAGKLGEE
                     LTGYFSTEESGKRLDLFPISEALRYRIPFSTAQEAIQYLESLASEPGTPAAIYFDDIE
                     KLGIWPETYAWVYEKQWLKQFIEGVLASPVIQPALYRDFHNLQHTRGVVYLPTTSYIE
                     MNEWTLNADRADRYAELIKREKDHNRYEQMKPFLRGGIWRNFMSRYAESNWMHKRMQQ
                     LSARLAVLDEAATPRMLELLHLTQANDAYWHGLFGGLYLPHLRRAVWNALIELEALLD
                     AATPRQPVASDLDMDGCDEFFIGNGTLQAVVRDDGLGAIHEFDDYRLRHNFGDTLARR
                     PEHYYRKIRDNDASHDRHKADGIASAHDRVNFRHPVSVADLVADRVPRVLFLDRQNGD
                     TVRYHRPRAQSSGSVKLSADRIEKRIAIESDRLTVEYKLESGVAPLQTEINLAMPSCD
                     GFLGRYVINGEVPGGFGQSFTWQGVTRVALEDRVLGGRVVLQCSQPVTVSAAPHRTVS
                     QSEDGFEKIMQAVTLMAIAPAGTYSLTLHSEILRD"
     gene            11762..11896
                     /locus_tag="VN496_17240"
     CDS             11762..11896
                     /locus_tag="VN496_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18755.1"
                     /translation="MEYRGMRILFYTGNNPEVFAHNAAAAGVDLGHLNFAIVSHRLSS
                     "
     gene            12033..12929
                     /locus_tag="VN496_17245"
     CDS             12033..12929
                     /locus_tag="VN496_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXF18756.1"
                     /translation="MAEQTSRRGFLRSSALGLGAGVTALAAAGCTGGVTATGPSANKL
                     NEVLKRGTVIVGTGSTNPPWHFEDEKGDLQGFDIEMGRLLAKGLFDDPAKVQFVRQAS
                     DARVPNLQTGQVDVVFQFMTVTAQRAQLVAFSIPYYREGVGTLLRRDSAHAGALDLAK
                     ARKKIKVSILQNVYAEGLVHAAIPQAEVMQFDSVANAVLALDAGRVEAAAIDDSTVRW
                     LVRQNPDKYKSGNYGWSPQTYAAAVRQGDPAWLNFVNTVLHESMTGVDFDAYASGFEK
                     YFGVKLAPPPVGFPVEFGLRHT"
     gene            12948..13610
                     /locus_tag="VN496_17250"
     CDS             12948..13610
                     /locus_tag="VN496_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXF18757.1"
                     /translation="MYQFNWRPVFDNFDLLLKGLGLAVAIALLSLTIGSLIGLAAAFG
                     RTHGPPPLQCLTTAYVELVRNIPLLLIIYFVYFGLPLLGVSMLDNLWSFVLALALYAG
                     AYLTEIFRAGIAGVGKGHIDAARSLGMTRLQVIRHITLPLMARVVLPSLGNTLISLFK
                     DTSVASAIAVPELTYGAIRINVNTWRVIEVYLVVAALYLATCYAIAGSLRWIERRWAI
                     VQ"
     gene            13577..14251
                     /locus_tag="VN496_17255"
     CDS             13577..14251
                     /locus_tag="VN496_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXF18758.1"
                     /translation="MDRAPLGNCPVMPMILAGLPLILHGLLVTATISLAVLLLGTVLG
                     IFGSLSLLYGANVLRWCVRIYVDVCRSIPHLVLIFAIFYGLPALGLQVPAIVAGILAL
                     GLFCGAHMSEIFRGGVESIPHGQMDAAKSIGLTFAQRLRHIIFPQALGRILPAWVNTA
                     VEIVKSSSLVSLVSVVDLTMAIQQIVGRTREALLFYAVAAMLYFAINFTLSITGRRLE
                     RKLAHG"
     gene            14238..15038
                     /locus_tag="VN496_17260"
     CDS             14238..15038
                     /locus_tag="VN496_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXF18759.1"
                     /translation="MPTADAMPEVNRNIVVLQGLAKRFAAAHILRGVNLEVTRGEVLV
                     IIGPSGSGKTTLLRCINFLEDYQGGRIFVDGELVGYEEADGKRTRRSDAAIARMRADV
                     GMVFQSFNLFPHMTALDNVMLGLCSVRKLSAAAARCSAASWLERVGLGDKMAAFPAQL
                     SGGQQQRVAIARAVAMEPKVMLFDEATSALDPELVNEVLGVMADLARSGMTMLVVTHE
                     MRFAREVGHRVVFMDQGVIVEQGKPQQIFDRPESDRLQAFLGHFAGNS"
     gene            15186..>16516
                     /locus_tag="VN496_17265"
     CDS             15186..>16516
                     /locus_tag="VN496_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXF18760.1"
                     /translation="MTKRKNPEELRSHRWYGAQDLRSFGHRSRTLQMGFAHEDYKGKP
                     VIAIINTWSDINNCHSHFRQRAEEVKRGVWQAGGYPLEMPAIALAEVFQKPTTMMYRN
                     FLAMETEELLRCYPVDGCVLMGGCDKTTPGLLMGAISMNIPAIFMPAGPMLRGNWQGK
                     TLGSGSDVWKYWDELRAGNITERDWTEIEQGIARSAGTCMTMGTASTMTSVAETLGFS
                     LPNSSTIPAVDSNHSRMADYSGRRIVEMVWEDLKPSDILTEKSFDNAIRVLMALGGST
                     NGLIHVIAMAGRAGIKIPLERFERIGQRTPYLVNMRPSGKYLMEDFYYAGGLAGMLEG
                     MRELLDTDQLTCTGKTLGENIAGAKIHNPDVIRTPDKPMQPEGGLIVLRGNLAPDGAV
                     IKSTAADQKLLKHAGRAMVFDDYNDMAARLDDPHLDVDANSVLVLRNAGPLG"
BASE COUNT         3413 a         4848 c         4838 g         3417 t
ORIGIN      
        1 gcgagcttgc gtcgttgcat gatctgatag cgcagaccga tggtagtggt ttcgttgaag
       61 caggcgtcga atacgctctc gaggtttggg atttcagcga gcaactgaat acgcatggtc
      121 agccggcctt ttttgccgaa agccggagtt tgcagcacat ccagcacaga cggatgcgca
      181 cgcagtctgt ccagcgcaat tgcaaggtct tcgggcgtct ggtcgtccac ttcgaactgg
      241 acctgcgcaa cttcgtcgcc gccatggctt tcccgggtct cgtcgaaggc aagcacgcgc
      301 aggacattgc tgatacccgg aaatgttctc gtaccgaaac cgatgccggt gcgcagcagg
      361 cggcgcgggc ggcgcgcagg tgcctgcgtg caaccgagat gccgaagaat cgcggcgcct
      421 gtaggtgtta cgcgctcacc ctcgaggcca tcatcggcaa actcgaaatc ccgaagcaac
      481 agcaccgtcg cgggtgatgg gacgggcagc atgccgtgtg ccgtcagtac cttgccacga
      541 ccaagcggca aggtactgac ggaccaagta gcacccagcg catcgatcaa atacgccgca
      601 ccgataatgt cggcaatcga atcccattcg ccaagttcgt gaaaactgac ggtctcgatg
      661 tctttgccat gcacttttgc ttcggcctca gcgagtacgc tgaacattgc gatggcgtga
      721 tcgcgcacgg ctggcgccag agcgcagcta tgcagatggg agcgaatgtc ggcgaagggc
      781 gtatgcgggt ggtgatgagc cgccaccagt aattcagctg tacgcggctc gatgatacgg
      841 aaacgcaaac cagtcagtgc gtgatcctga tgctccgcga aaccgacatg aacttcggcc
      901 ggaagaccag ctgcgcgaat tgccaccagc ataccgtccc gcaaatcggg gaacgcatcc
      961 agcatggcgg caataaacat atcaccggct acgccaccga ccgcgtcgag atggatatgc
     1021 atgcctgaac aattaagcca tgcgcagcga tcaggccgca gcgtgctccc gttgcttgcc
     1081 aagcatcgga tagagatttc ccttggcgtc gaacttaaaa ggttccgggc tgcccacaat
     1141 ctcgaattgc gacgggttcg ctttgataat tggcagcatc ggctcggaaa catggatatc
     1201 cataatttcc agggtcgtcg cgatgcgcac aatcctggtg tcctgcggct tgaccctgac
     1261 gaccgttttt accgccagcc gtatggcatc ttcgtcggtg ttcatgatga tcggaattgc
     1321 accaccatcg agatacgtcg acgtgatgat attcgcgtaa gtcttggcaa catcgagttc
     1381 cttgtacagc ttcatcgtcg tgacatccgc gccgccgatt ccgcatgcat tgccgtgcgt
     1441 ttcgggggtc agacccagca cggcgatttt tttcacaaaa ggcttcatgt tccattcgat
     1501 gaagcggcag ttgcgtccgg tgatgttggg atccatgccg gaaccggaaa tattcttgcc
     1561 gatcttctcc accaccagca cgtcgatttc gtcaaactgc aggcgcggca tgagttcctt
     1621 ggcttttgcc agcaataccg gctcgcgcgc aaaaatctgt tcggacggga cgatctcgat
     1681 gatagcggtc tcgtctgcgg cgttttcgac cattgcaacg ccgaacagca cgttcttctt
     1741 tgcaaggatg agtttcgccg tcttcggcaa taattcaccg aacatgtcca tgccataggt
     1801 atggagcgtg gttgcgccga tgattttgcc catgccgata gtcagcatct tgacgatgcc
     1861 ggactcgatc ggcgcgcgga aattcgtatg cggtttgacg cgcgggataa ttaccaccgc
     1921 gtcggcttca gatgcctgct tgtccatgta aaccggcatg ccgtcctcga gcttgcccaa
     1981 ttcaacgact tccatgctgg acttgatcgg gcagccaacg taactttcgg taatgccgta
     2041 gccctcgagc acttcacgtt gtccttcggc agtcgctccg ccgtggcttc ccatggcggg
     2101 aaagatgaac ggcttggcgc caagcgcctt cagttcgctg acgaccgctt tggcgcattc
     2161 ggcgatgttg ttgatgccgc gcgaaccgca accgacggcg atagtcatgc ctggcttaac
     2221 tttgctgcgc acttcggggc gcttgaactg ctcgacgatc gccttggtga cgctgtcgag
     2281 tttcttggtc gcgaacttct ggcgtacatg caccatgcgg ggcagcgcga tatcgagtcc
     2341 gccggccatg ttcacttcga tgcgatcacc gatcatttca gcctcctcca gtatcgtttt
     2401 ggcgccgcta accgttgcaa tggccatgca gcgaagaaaa gcattttact ccagtgcgct
     2461 agttcctacc cgatcgggag cgcccggtac agatcaaggt attgccgtgc gcgcgcttcc
     2521 cagctgaaat cgcgctgcat gccattcttc tgcagctgtc tccagcttct ctgttcagca
     2581 tagacgccaa cggctcggcg acagttccgc aacatggtcg gcgcatccag cggcgagaac
     2641 acaaaaccgg ttgccgtgcg gtttttcaac gtcgcaggga tgcagtcgac cactgaatca
     2701 agcagtccgc cggtcccgtg taccagcggg ggagtgccat agcgttgact gtacatctgg
     2761 ttaaggccgc atggttcaaa gcgcgaaggc atggcgaaaa tgtccgcccc cgcttcgatc
     2821 tggtgtgaca ggctctcatc gaatccaatc tgtacggcga tctggccggg atgatcggcg
     2881 gcaagttcct ggaatcgagt ctgcaacgct gggtcgccgg agccgagaac ggccagctgc
     2941 gcggggaatt ccactaggtc tgccgcgatg tccgccaata cgtcgagtcc tttttggccg
     3001 gtcatacgac ttaccgaacc gaataatggc gtcagcggcg catcctgcaa tcccatgcgc
     3061 agttgcagcg cccgcttgtt gtcctgctta gccgccagcc gggacgcgtt gtaatagcgc
     3121 tcgatatacg gatccgactc cggatcccag gcgtcgacgt cgatgccgtt gaggataccg
     3181 gtcagcacgt ttgcgcgatg cgatagcagg ccctggaagc ccatgcccag gggctcgctt
     3241 tggatttcgc gcgcatagct tggacttaca gtcgtgatgc gatcggcgaa ctgcagtccg
     3301 cccttcagga acgaaaggtt accgtaaaac tccacgccat caatggtgaa tgcttgcgga
     3361 ggcaagccga ggcgatttgc gctttgctgc gggaaaatac cctggaatgc cagattgtgg
     3421 accgtcatga tggtgcgagc gcgcgcgccc tgcacgtaat tcaggtatac cggtgtaagg
     3481 ccggtctgcc agtcgttgca gtggacgata tgtggttgcc atgccgcaaa cggtgagctg
     3541 ggtgtcgaca gcagcgcacc cacataggag aacagtccaa agcgcaggtc gttgtcgggc
     3601 cagtcggtgc cattgctatc ctgatagggc ccgccgccac gatcgtaaat gggacactcg
     3661 acgacgaaca atggcaatcc ggatggaagt ttcgatgcaa ccaggctggc ggcgggaaca
     3721 ccaggcagcc ctggcagcgc cgcgacgcga ctcttcgttt tgagcgcctc catgacttgt
     3781 ggataccccg gtaccagcac acggacgtcc acgccaagcg catttaacgc caccggcagc
     3841 gccgcgctga cgtccgcaag tccgccggtc ttgatcaggg gcacgacttc cgaagtcgca
     3901 aatagcactt tcagtttttc ggcggcagcc attgttggtt ttatggtcag gagaattcag
     3961 tgtttcgctg tgccgtagac ctggcatgca taaggaccac tgccttagag cgcgactcgc
     4021 tgactacaga catgcgccag cctggcgcct ctggacgctc atgccgttca cggtgcgtcg
     4081 cagcgcagga tcagcgcgcc caatgggggc agcgttatgt cggccgattg accatgcccc
     4141 atccacggaa agggctgcga ttgaatgatt cccgcattgc ccgaattcga gccggcatag
     4201 aaagaggaat cgctattgaa tacttcccgc cacgcgccgg gtctgggcag gccaacgcga
     4261 taaccgtgcc gcggcaccgg tgtgaaattc aacgccacca cgacgcccga accatcgcgt
     4321 gcgcgacgtt cgaaacacag caccgactgg tcagcatcat tgcagtcgat ccagcgaaag
     4381 ccttccggct cgaagtccaa ttcatgcaat gcggcttcgt tgcggtacag gcggcaaaga
     4441 tcgcgcacca tggactgcac gccttggtgc cagccaatgc caagcagccc ccagtcgagt
     4501 tctgcgctgg cgcgccattc gcggccctgc gcaaactcat tccccatgaa attgagcttc
     4561 ttgccagccg aggcaaactg ataagcgatt agcagccgga cattggcaaa cttttgccac
     4621 gcgtcgccgg gcatcttgga cagcagcgac cctttgccgt gcacgacttc gtcgtgcgaa
     4681 aggggcagca aaaagttttc gctgtacgcg tagatttgtc cgaatgtgag ttgattatgg
     4741 tgaaaacggc ggtgcaccgg atcgtttttc atgtaggaca aggtgtcatt catccagccc
     4801 atgttccact tcatagaaaa ccccaggccg cccagataga ccggacgcga aaccatgggc
     4861 cacgcagtgg attcctcggc gcaggtcagc gcaccgggaa agtcgcgatg gaccattacg
     4921 ttcaattcgc gcaagaacgc gatggcatcc aggttttccc gacctccgtg cttgttggga
     4981 atccattcgc cggccttgcg tgagtaatcc aggtaaagca tcgaggcgac tgcgtcgact
     5041 cgcaagccat ccaggtggca ttccgcaagc cagtagtgcg cactcgacag caggaagctc
     5101 ttcacctcat tgcggccgta attaaagatg tgcgtgcccc agtcctgatg gaaacccagg
     5161 cgcggatcct catgctcgta aagcgcggta ccgtcaaacc gcgccaggga aaaagcgtcg
     5221 gtcggaaaat gcgccggcac ccaatccaga atgacgccaa taccggcctg gtggcaggcc
     5281 tccacgaatg ccttgaatgc gtcgaccccg ccaaaccggc tggtcgccgc gaaatatcca
     5341 gtggtctggt agccccagga ttcgtccagc ggatgttcgg atacgggcaa cagttcgata
     5401 tgcgtaaatc ccatttccag gacgtacggg atgagactct gcgccagttc tgcataggtg
     5461 tagaaacggc catcagggtg acgccgccag ctgccggcat ggacttcgta aatattgacg
     5521 ggagcatgaa gccagtccca acgcgagcgc ttgcccatcc attcctcgtc gcgccagaca
     5581 tggtcattcg caacgacttg cgcagcgtta ccgggacgca attcatgcgc gcgcgcatac
     5641 ggatcggtct tgacgaaaac ttcacccgtg ctgcgattgc ggatttcgta gcgatacagt
     5701 gcgccaacgt cgatgccggg aacgaacaac tcccagactc cactcaggcc atgcgaggac
     5761 atcgggttgg tacgtccgtc ccagcggtta aactctccga ctacgctgac ccgtgctgca
     5821 ttcggtgccc agcaggcaaa gcgaaccccg cggacgccct cgcgctgctc aacatggctg
     5881 ccaagcatgc gatacgcttg ctcgagccgg ccctcattga acagatacaa atcgtccgcg
     5941 ccaatgaccg gcgcaaacgt gtacgggtcc aacgtctcga attcgcgggc ggattctttc
     6001 accttcaaag taacgggatg aggtggcggc tcacgacctt gccaggcgaa gattccgagc
     6061 ggatggacgc gtttcatggc agcgaatccg ctctgggtgc gtactgctat gctgctggcg
     6121 taaggacgga acacgcgcag agaccagtcc gagccggcac gatgcagtcc gagcacgctg
     6181 aacggatcat ggtggcgggc gtgcgccaag gcgtcgagat cgtcggcggc gggtaattgg
     6241 gcagtagcag gcatggtggc gggaagggac agcgttcggt actggaattc gtgacgacgg
     6301 gcgacgtcac ccctattata agcagagcac tgcgcttgcg gactttcttt gatggtaacg
     6361 atgaaccgga tagcaggtgc cgcggtcggt caggcatggg cataggccgg aaggcgggat
     6421 cccgaatccg atcattcaag atgcaccagg aaccttatga ttgacaccga cctgaatgtc
     6481 cgcttcgtca gcttgctgac caagaatact tacgcgctgg tactggcagg gggacgcgga
     6541 agccggctta aaaatctgac cgactggcgc gccaaaccgg ccgtgccctt cggtggcaaa
     6601 ttccgcatcg tcgatttccc gatgtcaaac tgcgtgaatt ccggcattcg ccgcatcggc
     6661 gtggccacgc agtacaagtc gcacagcctc attcggcaca tccagcgcgg ctggagtttt
     6721 ctcgatggac gcttcgagga attcgtggac atcctgcctg cctcgcagag aacggcagaa
     6781 gaaaactggt atctcggtac ggccgatgcg gtttaccaga atctggacat tctgcgcgcc
     6841 tatggtccgg actatatcct cgtgctggcg ggcgaccaca tctataaaat ggattacgga
     6901 aaaatgctcg cttaccatgt cgccaagaac gctgacatga cggttgcgtg cctcgaagtg
     6961 ccgcggatgg aagccactgc gttcggcgtc atgcacatca acgaaaactg gcgcgtcacc
     7021 gacttcgtgg aaaaacctgc cgatcctccc tccataccgg ggcgggccga catggcattg
     7081 gccagcatgg gcatctacgt attcaatgcg ccgttcctgt atgagcagct tgcgcgcgac
     7141 accgcgacgc caaattccag ccacgatttc ggcaaggatc tcattccgca cgtggtgcca
     7201 cgctacggcg tttacgcgca gcgcttcgac gacagctgcg tcggccaggg cggccagcct
     7261 gcctactggc gggacgttgg gacggtggac gcgtactggg agtcgaacct ggatctgacg
     7321 cgagtgaccc ccgatttgaa catgtacgac aaggactggc cgatttggac gcaccaggaa
     7381 caattacctc cggccaagtt cgtattcgac gatgatggcc gccgcggctc cgctgtcgac
     7441 tcggtcgtat ccggcggttg cttgatcagt ggaacctcca tcaagcggtc cctgctattt
     7501 tcaaacgtcc gtgtacacag ctacggcagc atcgatgact ccgtaatact cccggacgtt
     7561 atcgtgaacc ggcatgtcaa actccgctgc accatcgtgg acaagggctg tgtactgccg
     7621 gaaggtttaa ccgtaggttt caacccggaa caagaccgcc ggagattcca tgtgtcggag
     7681 cgtggtatca ccctgatcac acccgaaatg cttggacagg aagttcttca ccaacgctaa
     7741 aacggtacgc cgggcgcaac cgttagtcat tcctctcatt cacatttatg atcgccgaga
     7801 aaaaactcga cctggccatc ctatggcaca tgcatcagcc ggacttccgc gaccatgcga
     7861 caggtgagtt cgcgcagccc tgggtgtatc tgcacgccat caaggattac agcgacatgg
     7921 ccgcgcatct cgaagcccac ccgggtgtgc gcgttacact taacttcacg ccggtattac
     7981 tggaccagtt ggaggactac agcgaccaat tctcgaccgg acagttccgc gatccgctgc
     8041 tgcgcctgct tgcgcaaccg catttggagc aggtcggccc agttgaacgc aatcttgcac
     8101 tggcggcctg cttcaaggct aaccggcaga agctgatcga tccgtatccg gcctacaaaa
     8161 ggctgctgga attcgcgcgt ctggcgcagg agcagggcga tgccggcgca agctatctgt
     8221 cggcgcagta tctctctgac ctgttgagct ggtatcacct ctcctggatg ggcgaaacag
     8281 tgcgccgtca gtacccggtg ttgcccgcac tgatcgccaa ggcacatggt ttttcgcacg
     8341 acgaccgcat gcaattgctg cacatcattg cgcacgaggt acgccaggtt attccgcgtt
     8401 accgcacgtt gttcgaagca ggacgcatcg aactgacgac cacaccggaa taccatccga
     8461 tcggcccatt gctgctgtcc tttgaatcag cccttgaagc agcacccggc tcgccgctgc
     8521 cgcagagcac acattatccg ggcggcgacg aacgtgcgcg cgcgcagacg gaagctgcgc
     8581 agcaaagcca cgcgaggcgc ttcggccggc aaccgagagg catgtggccc gcagaaggcg
     8641 cagtgtcgag cgattttgcc aatctgctgg cggaggccgg attcgaatgg attgcgagcg
     8701 gcgagggcgt tctggttcac actttgaaag aacacggtga cgcactatcc gagcggccgc
     8761 agtatctata ccggccttac cgcctggaga aaggccagaa caccatcgtc tgtttctttc
     8821 gagacgaccg gctctctgat ctgattggct tcgaatatgc aaaatggcat gggaaagacg
     8881 ccgccgaaca tttcgttgca gccctttcgg acattgccaa tggcgtgacg gaccagcaac
     8941 ggccagtggt cagcgtgata ctggatggcg aaaatgcctg ggagtattac ccctacaatg
     9001 gctactactt cctggacgag ctttatggcg cgctggaagg ccatggggaa atagccacca
     9061 ctacctttac gcaactgctc gatgaaaaac gtgaagccac gccgctgcct gcattggtag
     9121 ccggcagctg ggtatacggt aatctggcca catggatcgg cagtgccgac aagaaccgcg
     9181 catgggatct actatgcgcg gcgaaacaaa gttttgatgc cgttagccac agcggccgcc
     9241 tcgaaggcgg cgagctgaaa cgcgccaggc gccagctggc cgattgcgag gcgtccgact
     9301 ggtgctggtg gttcggagat tacaatccag cggaatcggt aatggcattc gatgcgctat
     9361 tccgtagcaa gctcaggcat ctttacggac tgctaaaact gcccgcgccc gcggagctca
     9421 acctgcccgt aagccgcggc aaggcagact cggaagccgt caatgcgatg cgccgtgcat
     9481 cgtaactgcg ccggcgccgg ttcggctttc agttcgtggt gttgcgcaca gcggacggca
     9541 ccacagaacc ctggggatga aacggccaca tgaacaagaa agtctcgctg ctgttcggtg
     9601 tacacgcgca ccagccggcc ggcaatttca taggggtaat cgacgatgcg catgcgcgtt
     9661 gttacaaacc tttcctcgaa accgtgcatg gatacccgca gttcaaattg tcggttcact
     9721 tcagcggctg gctgctggat gtattactcc agagatttcc ccaagacatg gcgaaactgc
     9781 gcgacatggt ggggcgcggc caggtcgaaa tgttcggtgg aggcgacacg gagccggtac
     9841 tggcgtcgat tccggagcgc gaccgccggt cgcagcttgc ggctttgtcc agccggctgg
     9901 aagcgtcttt gggccagcgg ccacgcggcg cctggcttac cgaacgggtg tgggaatcat
     9961 cggtcgcctc ggcgctggtc gattccggga tccgttatgt caccgtggat gactatcatt
    10021 ttctctgcgc cggcaagctc ggcgaggagt tgaccggtta tttctcaacg gaagaaagcg
    10081 gtaaacgcct ggatcttttt ccaatctcgg aagcgctgcg ttaccgtatc ccgttttcca
    10141 ccgcgcagga ggcaatccag tatctcgaat cgcttgcctc tgagcccggt accccagccg
    10201 ccatctattt cgatgacatc gaaaaactcg gcatttggcc ggaaacctat gcgtgggtat
    10261 acgaaaagca gtggctcaag cagttcatcg aaggcgtgct ggcctctcca gtcatccagc
    10321 ctgcgctgta tcgggatttc cataacctgc agcacacgcg cggcgtggtg tatctgccga
    10381 ctacttcata catcgagatg aatgaatgga cgctcaatgc cgaccgtgcc gaccgctatg
    10441 cggaactgat caagcgtgaa aaagaccaca accgctatga gcagatgaaa cctttcctgc
    10501 gtggcggcat ctggcgcaat ttcatgtcgc gctacgcaga atcgaactgg atgcacaagc
    10561 gcatgcaaca gctctctgcg cgtctcgctg ttcttgatga ggccgcaaca ccgcgcatgc
    10621 tggaactact gcacctgact caggccaatg acgcgtactg gcacgggttg ttcggcggtc
    10681 tttatttgcc gcatctgcgc cgcgcggtgt ggaatgcgct gatcgagctg gaagcactgc
    10741 tcgacgctgc cacgccccga cagccagttg cttccgacct cgacatggac ggatgcgatg
    10801 agtttttcat cgggaacggt actttgcagg ccgtggttcg cgacgacgga ttgggtgcga
    10861 ttcacgagtt tgatgactac cggctgcgac acaactttgg cgatacgctg gcacggcggc
    10921 ccgagcacta ctatcgcaag atccgggata acgacgcctc acacgatcgg cataaggcag
    10981 acggcatagc gtccgcgcac gatcgcgtca attttcgtca tccggtatct gtcgcggatc
    11041 tcgtggcgga tcgggtgccg cgcgttctat tcctcgatcg ccagaatggc gacacggttc
    11101 gataccaccg gccaagggcc cagagtagtg gcagcgtcaa gctatccgca gacagaatcg
    11161 aaaaacgcat cgcgatcgaa agtgaccggc ttacggtcga atacaagctt gaatctggag
    11221 tggcgcccct gcaaaccgag atcaatttgg ccatgccaag ttgcgatggg tttctcggcc
    11281 gctacgtgat caatggcgaa gtgcccggcg gttttggtca atcgttcacg tggcaaggtg
    11341 ttacccgcgt tgcactcgaa gatcgcgtac tgggtggtcg cgtggtactg caatgcagtc
    11401 agccagtcac agtcagcgcg gccccgcacc gtacggtgtc tcaatcggag gacggtttcg
    11461 agaagatcat gcaagccgtc acgttgatgg cgattgcgcc agccggcact tattcgctta
    11521 cattgcactc ggaaatcttg cgcgattgac acgccaacgc cagcggcgaa catggcttgg
    11581 tggcgcggta ctgcgccggc gttcgtgagg tcatgcaggt ggctgcgtcg ctttttcttt
    11641 cgttgccttg ctggcactgg ccaccccttg cttaggctcc gacggcaccg cgtcaccatt
    11701 ctgtacgacg cattcgggga cgcctccggc ctcaccaaga actggggatt cgggtgcact
    11761 ggtggaatac cgcgggatgc gcattctctt ctacaccggc aacaacccgg aggtgtttgc
    11821 ccacaatgca gccgctgccg gagtggatct tggccatctc aatttcgcga ttgtttcgca
    11881 ccggttaagt tcgtaaagca gcctggacgg tgctaaagca tcgtcggtgc aacgccacat
    11941 cgcgttcgct ggcaatccga cccgaagtcg tctataaatc tgcgaacctc caatttggag
    12001 ggcagcgtcc agtttcaaat gaggaggcgg aaatggcgga gcaaacttcg cgtcgcgggt
    12061 tcctgaggtc gtctgcgctc ggattaggcg caggtgtcac cgcacttgcg gccgcaggct
    12121 gcactggtgg cgtcaccgcc accggaccaa gcgcgaacaa actgaacgaa gtcctcaaac
    12181 gcggcacggt gatcgttggc accggcagca ctaatccgcc ctggcatttt gaagacgaaa
    12241 agggagacct gcagggcttc gacatcgaaa tgggccgttt gctggccaaa ggcttgttcg
    12301 atgacccggc caaggttcag ttcgtgcgtc aggcgtccga cgcccgagtg ccaaatttgc
    12361 agactggtca agtcgatgtc gtatttcagt tcatgacggt taccgcccag cgcgcgcagc
    12421 tggttgcgtt ctccatcccc tactatcgag aaggcgtcgg cacgctattg cggcgggaca
    12481 gcgcacatgc gggcgcgctc gatcttgcca aagctagaaa gaagatcaaa gtatccatcc
    12541 tgcaaaatgt ctatgcggag ggcttggtgc acgcggcgat tcctcaggcg gaagtgatgc
    12601 agttcgactc tgtagcaaac gcggtgctcg cactcgacgc ggggcgggta gaagcagctg
    12661 caatcgatga ttcgactgta cgctggctgg tgcgccagaa cccggacaaa tacaagtccg
    12721 gtaactacgg ctggtcgcca cagacctatg ctgcggcggt acggcaaggg gaccccgcgt
    12781 ggctgaactt cgtcaacacc gtgctgcacg aaagcatgac cggcgtcgat ttcgacgcct
    12841 atgcgagcgg atttgaaaaa tacttcggcg tgaaactggc gccgccgcca gtgggtttcc
    12901 cggtcgagtt cggcttgcgc cacacgtagc atcgtcaggc cggaaccatg taccagttca
    12961 actggcgccc ggtcttcgac aatttcgatt tgctgcttaa agggcttggc cttgccgtgg
    13021 ccattgcctt gctatcgttg acaattggca gcttgatcgg actcgcagca gcctttgggc
    13081 gcacgcacgg gccgcctcct ttgcagtgtt tgactaccgc ctatgtcgag ctggtacgca
    13141 atatcccgct actgctgatc atctacttcg tctacttcgg cctgccgttg ctgggtgtca
    13201 gcatgctgga caacctctgg tccttcgtcc ttgctctggc gctgtacgcc ggtgcctatc
    13261 tgacggaaat ctttcgcgcc ggcatcgccg gcgtcggtaa agggcacatt gacgccgcgc
    13321 gctcacttgg tatgacgaga ctccaggtga ttcggcatat cacactgccc ctgatggccc
    13381 gcgtcgtgct gccttcgctc ggcaatacgc tgatttcgct gttcaaggat acgtcggtgg
    13441 cgtcagcgat cgcggtcccg gaactcacat atggtgcgat ccgtatcaat gtaaatacct
    13501 ggcgcgtcat cgaggtctac ctcgtcgtgg ccgcgcttta tctcgcaact tgttacgcca
    13561 tcgcgggatc gctgcgatgg atcgagcgcc gttgggcaat tgtccagtga tgccgatgat
    13621 actggccggt ttaccgctca tcctgcacgg cctgctggtc accgccacga tttcgcttgc
    13681 ggtcctgctc ctgggaacag tgcttggcat ttttggcagc ctgtcgctgc tgtacggcgc
    13741 aaatgtcctg cgctggtgcg tgcgcatcta tgtcgatgtg tgccgcagca tcccacacct
    13801 ggtgctgata tttgcaatct tctacggttt gccggcactg ggcctgcagg ttccggccat
    13861 cgttgccggc attctggcgc tgggactatt ctgcggcgcc cacatgtcgg agatttttcg
    13921 cggtggcgtc gaatcgattc cgcacggaca gatggatgcc gctaaatcca tcggactgac
    13981 tttcgcacag cgcctgcgcc atataatttt cccgcaggcc ctcggccgta tcctgccggc
    14041 ctgggtcaac acggctgtcg aaatcgtgaa gtcctcctcg ctggtttcac tggtcagcgt
    14101 ggtggacctc accatggcga ttcagcaaat cgtcggacgc acgcgcgaag ccctgctgtt
    14161 ttacgcggtg gcagccatgc tctatttcgc aatcaatttc acattgtcca tcaccggccg
    14221 gcgtttggag agaaaacttg cccacggctg acgccatgcc agaggtgaac cgcaacatcg
    14281 tggtgttgca ggggcttgcc aaacgcttcg cagcggcgca catactgcgc ggcgtgaacc
    14341 tcgaagtaac ccgcggcgaa gtattggtca ttatcggtcc gagcggctcc ggcaagacta
    14401 cgctgctgcg ctgtatcaac ttcctcgagg actaccaagg cggccgcatt tttgtggatg
    14461 gtgaactggt cggttacgag gaggccgatg ggaagcgtac ccggcgttca gatgcggcaa
    14521 ttgcgcgcat gcgcgctgat gttggcatgg tattccagag tttcaatctg tttccgcaca
    14581 tgacggcgct ggacaatgtg atgctgggac tgtgcagcgt acgcaagctt tccgccgcag
    14641 ccgcgcggtg cagtgccgcg tcctggctgg aacgcgtcgg tctgggggac aagatggctg
    14701 cttttcccgc gcaactctcc ggcggccagc agcagcgcgt cgccattgcg cgggcggtcg
    14761 ccatggaacc aaaagtaatg ctgttcgacg aggcgacttc tgcacttgat ccggaactgg
    14821 tgaatgaagt gctgggcgtg atggcggatc tcgcccgcag cggcatgacc atgctggtcg
    14881 tcacgcatga aatgcgtttt gcccgcgaag tcggccatcg cgttgtattc atggaccagg
    14941 gcgtgattgt cgaacaaggc aagccgcagc agatttttga cagaccggag tccgaccggt
    15001 tgcaggcctt cctcggccat ttcgccggca acagctaaaa cgcacgcgct tggattgcgg
    15061 tctcgctgcg cccctgaagc cgcttcaccc atgcagcgcc cgctcgaccg ctgcatctat
    15121 gccgcgcagc gcacctgttg taggcttcct gcattcgttt agccgcgcat ggaatggaga
    15181 gaagcatgac taaaaggaaa aacccggagg aattgcgcag ccaccgctgg tacggcgcgc
    15241 aggatctgcg ctccttcggt caccgttcgc gcacgctgca aatgggattc gcgcacgaag
    15301 actacaaggg caagccggtt atcgcgatca tcaatacctg gagcgatatc aacaactgcc
    15361 attcgcactt tcgccaacgc gccgaggagg tcaagcgcgg cgtctggcag gcgggcggct
    15421 acccgcttga gatgccggcc attgcgctgg cagaagtttt ccagaaaccg accacgatga
    15481 tgtaccgcaa cttcctggcc atggaaaccg aggaactcct gcgctgttat cccgtcgacg
    15541 gctgcgtgct catgggtgga tgcgacaaga ccacgcccgg gttattaatg ggcgccatca
    15601 gcatgaacat tcccgcgatc ttcatgcctg ccgggccgat gctgcgtggc aactggcaag
    15661 gcaagacgtt aggctcgggg tcggacgtct ggaaatactg ggatgaactg cgggcaggca
    15721 atatcactga gcgtgactgg acggaaatcg agcaaggcat tgcgcgctcg gccggtacct
    15781 gcatgactat gggtacggcc tcgaccatga caagcgtcgc ggaaacgctc ggcttcagct
    15841 tgccaaactc ttcgaccatt ccagccgtcg actcgaacca tagccggatg gcagattaca
    15901 gcggcaggcg catcgtcgaa atggtgtggg aggatctcaa accgagcgac atcctcaccg
    15961 agaaatcgtt tgataacgcc attcgcgtgc tgatggcgct gggcggctcc acgaatggat
    16021 taattcacgt aatcgccatg gcaggccgcg ccggcatcaa gattccgctg gagcgcttcg
    16081 agcgaatcgg ccagcgcaca ccctacctcg tgaatatgcg gccctcgggc aagtatctca
    16141 tggaggactt ttactatgcc gggggcctcg ccggcatgct ggaaggcatg cgcgaattgc
    16201 tcgacaccga tcagctaacg tgtaccggaa aaacactggg ggaaaacatc gctggagcca
    16261 aaatccataa tcccgacgtc attcgcacac cggacaagcc catgcagccg gaaggcgggc
    16321 tgatcgtgtt gcgaggcaat ctcgcgcctg atggcgcagt cattaaatca accgccgctg
    16381 atcagaaatt gctaaaacat gccggccgcg ccatggtgtt cgatgactac aatgacatgg
    16441 ccgcgcggct cgatgatccc catctggatg tcgatgccaa ttcggttctg gtgctgcgca
    16501 atgcaggtcc gctcgg
//
LOCUS       DATMZQ010000539         4867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_2410533, whole genome shotgun sequence.
ACCESSION   DATMZQ010000539 DATMZQ010000000
VERSION     DATMZQ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410533"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VN496_17270"
     CDS             complement(<1..830)
                     /locus_tag="VN496_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXF18761.1"
                     /translation="MSGPPANKKESFLRKLGPGLITGAADDDPSGIATYSQAGAQFGL
                     DMLWTSLVTFPLMVGIQIVSARIGRVTGEGLAANIRRLYPRWVLLMVVALLVVANTIN
                     IAADIAAMGEALQLVIGGGEHGHAIIFGVVSLLLQVFIPYHRYVRVLKWLTLSLLAYV
                     AVAFSVHIDWARAIQQSFAPTLSFNPDFIAVVVAVFGTTISPYLFFWQASQEVEELRG
                     GHGSSLKSTPVDARAHLRRIKIDTYVGMGFSNLIAFFIILSAAVTLHVAGITEIRTSA
                     "
     gene            complement(946..4053)
                     /locus_tag="VN496_17275"
     CDS             complement(946..4053)
                     /locus_tag="VN496_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXF18762.1"
                     /translation="MSIAEIFVRRPVMTGLVMIGILAFGIVSYRLLPVSDLPNIDFPT
                     IAVTASLPGASPETMASAVATPLEKEFSTIAGLDSMDSTNAQGISRVTLQFSLDRNID
                     AAAADVQAAITRALKQLPPQMTTPPAFQKVNPADAPIFYLALTSTTLPMSTVDEYAQT
                     LLAQRISTISGVAQVQVLGSQKYAVRIQLDPKALASRGIGIDEVAEAINHHNTNLPTG
                     TLYGANRAYTVESNGQLMNAAAFRPVVIAYRNGAPVRLNEIGRVIDSVENDKLAAWLN
                     DERGIVLAIQRQPGTNTIEIVNSIKRLLPTFRAQAPAGLKMDILYDRSDSVRESVDDV
                     QFTLMLALALVVLVIFLFLRNISATLIPSLALPMSIVGTFSVMYLLGYSLDNLSLMAL
                     TLCVGFVVDDAIVMLENISRHMEMGKPPMQATLEGAREVGFTIVSMTLSLAAVFIPVL
                     FMGGILGKLLHEFAVTIMAAVLVSGFVSLSLTPMLCSRWLRPVAHNRHGKLYRWSEGA
                     FDAVRNLYDHTLKWSIRYSWFTMLVFFAIFAATAWLFSIMPKGFLPTTDTGQLLIFTE
                     AAQDISFEAMAAKQRQAAKIVQADPNVEAAMAFIGPSSSGSSQTLNLGRIFIRLKPRK
                     QRLPADAIIQELRPKLQSIPGMKVYLQNIPPVRIGGKVTKSEYQYTLQDAETQELYHW
                     APLLESKMRSLPAFIDVNSDLQITNPTVKVQIDRDKASALGVTAGQVEDALYSAFGTR
                     QVSTIYTPSNEFFVIMELAPEYQASPEALSLLYVRAGTGTLVPLSSVARIERGVSPLT
                     VTHQGQLPSVTLSFNLKPGVSLSEAIAQLNGLITELRIPPTVIGSFQGTAQAFQSSLA
                     GMGMLLIMSIFVIYLILGILYESYIHPITILSGLPTAGFGALLTLMLFKLDLNMYGFV
                     GLIMLIGIVKKNAIMMIDFAIEAQRRDGKRPEQAIYEACLIRFRPIMMTTAAALMGTL
                     PIALGFGAGSEARRPLGLAVVGGLLVSQLLTLYITPVIYLYLERMRTWQKGRRARGAE
                     VTAGI"
     gene            complement(4050..>4867)
                     /locus_tag="VN496_17280"
     CDS             complement(4050..>4867)
                     /locus_tag="VN496_17280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXF18763.1"
                     /translation="QSQLEQAQANLARDQAQLEYLRGQELRYQDLLKQNFVSKEGYAQ
                     VAANFHGAQASVVADEAAVQHARIDLGYTTIRSPIDGRAGKVMLSVGNMVKANDTLPL
                     VVINQLTPIYVSVAVPEQYLNEIREHHKKAALRVQVEVEKGETINGKLAFIDNAVDVS
                     TGTIKLRGIFANEDKRLWPGQFVESWVTLRDEDKALVVPTQALQTGPNGQYVYVVKQD
                     STVELRLVKVARTEGVDSVVAEGLQQGETVVVDGASRIVPGSKVSAKTPEKTS"
BASE COUNT          994 a         1433 c         1481 g          959 t
ORIGIN      
        1 tcggccgaag tccggatctc ggtgatgccc gcgacgtgca aggtcaccgc ggcggacagg
       61 ataatgaaga aagcgatcag gttggaaaac cccatgccga cgtaagtatc gatcttgatg
      121 cgacgcaggt gggctcttgc atcaaccggc gtgcttttca gcgatgagcc gtggccgcca
      181 cgcagctcct cgacttcctg cgatgcttgc cagaagaaca ggtaggggct gatggtagtg
      241 ccgaacactg caacaacgac agcgatgaaa tcaggattga acgacagggt tggcgcgaac
      301 gactgctgga tggcacgcgc ccagtcgata tgcaccgaga acgccaccgc cacgtaagcc
      361 agcagcgaaa gcgtcagcca tttcagcacg cgcacgtaac gatggtaagg gatgaatacc
      421 tgcagcagca ggctgaccac gccaaagatg atggcatggc cgtgttcgcc accgccgatg
      481 acaagctgca gcgcctcgcc cattgctgcg atatccgcag cgatattgat ggtgtttgcc
      541 accaccagta gcgcaactac catcagcagc acccagcgcg gatacagccg gcggatgttg
      601 gccgccagac cttcgccggt gacccggcca atacgggcac tcacgatctg gatgcccacc
      661 atcagcggga aggtgacgag cgaagtccat agcatgtcca gtccgaattg cgcgccggcc
      721 tgggaatagg tggcgatgcc gcttggatca tcgtccgcgg ccccggtgat aagacccggt
      781 ccgagtttgc gcaggaatga ttcttttttg tttgcaggcg gtccgctcat gtgttttcca
      841 ggagcacaaa gcgatgagca aatcttgctc gtgctgtgcc gccgctctaa cgccggttca
      901 cgggagtcgt tgacgctgcg ccgctatgcg agcgcatgcg ccgcgttaaa tgcccgcggt
      961 tacttcggcg ccgcgcgcac gccggccctt ctgccaggtg cgcatccgtt ccaggtaaag
     1021 atagatcacc ggcgtaatgt acaaggtcag cagttgcgac accagcaatc cgccgacgac
     1081 cgcgaggccg agcggcctgc gtgcttccga tccggcgccg aatccaagcg cgatcggcag
     1141 agtacccatc agcgccgctg cggtcgtcat catgatcggc cggaagcgga tgaggcaagc
     1201 ctcgtagatc gcctgctccg ggcgcttgcc gtcccggcgc tgcgcttcga ttgcgaagtc
     1261 gatcatcatg attgcgttct tcttgacgat gccgatcagc atgatcagtc cgacgaaccc
     1321 gtacatgttc agatcgagct tgaacagcat cagcgtcagc agcgcgccaa aaccggcggt
     1381 cggcaggccg gacaggatgg tgatcgggtg aatatagctt tcgtacagga ttccgaggat
     1441 caaatagatc acgaaaatgg acatgatcag cagcatgccc atgccggcca acgacgactg
     1501 gaatgcttgc gcggtaccct gaaagctccc gattacggtc ggcgggatac gcagttcagt
     1561 gatgaggccg ttgagttgcg cgatggcctc gctcaatgag acgcccggtt tcaggttgaa
     1621 cgacagcgtg accgaaggca actgcccctg gtgggtgacc gtcagcgggc tcaccccgcg
     1681 ttcgatgcgg gccaccgacg acagcgggac cagcgtgccg gtgccggcac gcacgtatag
     1741 cagggacaat gcttcagggc tcgcctggta ctccggtgcg agttccatga tgacgaagaa
     1801 ctcgttactc ggtgtataaa tggttgacac ctggcgcgtg ccgaatgcac tgtacagcgc
     1861 atcttcgacc tggccggcgg tgaccccgag cgcggacgcc ttgtcgcgat cgatctgtac
     1921 ctttaccgtc ggattggtga tctgcagatc gctattcacg tcgatgaacg caggcaggct
     1981 acgcatcttg ctctcgagca acggcgccca gtgatacaac tcctgggtct cggcatcctg
     2041 cagcgtgtac tggtattcgc tcttggtgac cttgccaccg atgcgtactg gcggaatatt
     2101 ctgcaggtac accttcatgc cgggtatcga ttgaagcttt ggccgcaact cctggatgat
     2161 ggcatctgca ggcagccttt gcttgcgcgg tttcagccgg atgaaaatcc ggccgagatt
     2221 cagcgtctgg ctcgatccgg atgaagaagg cccgatgaac gccatggctg cctcgacgtt
     2281 cgggtcggcc tgaacaattt tggcggcctg ccgctgcttc gctgccatgg cctcgaagga
     2341 aatgtcctgt gcggcctcgg taaagatcag caactggccg gtgtccgtag tgggcaggaa
     2401 acctttcggc atgatcgaga acagccacgc ggtggcggcg aaaatcgcga agaacaccag
     2461 catagtgaac cagctgtagc ggatggacca tttcagcgtg tggtcataca ggttgcgcac
     2521 ggcgtcgaac gcgccttcgc tccaccggta gagtttgccg tggcgattgt gcgcgaccgg
     2581 ccgcagccag cggctgcata gcataggtgt cagggaaagc gacacgaagc ccgataccag
     2641 cacggccgcc atgatggtca ctgcgaactc gtgcagcagc ttgccgagaa tgccgcccat
     2701 gaacagcacc ggaatgaaca ccgcggcaag cgacaaagtc atcgacacga tagtgaagcc
     2761 gacctcgcgc gcaccttcca gcgttgcctg catgggcggc ttgcccattt ccatatgccg
     2821 gctgatattc tccaacatga cgatggcgtc atcgaccacg aaaccaacgc acagcgttaa
     2881 agccatcaac gaaagattat ccaggctgta gcccagcaga tacatcaccg aaaacgttcc
     2941 gacgatggac atcggtagcg cgaggcttgg aatgagcgtg gcggagatgt tgcgcaggaa
     3001 cagaaagatc accagcacca ccagcgcgag cgcaagcatc agggtgaact gcacgtcgtc
     3061 cacggattcg cgcacggaat cggaacgatc gtaaagaatg tccattttga gaccagccgg
     3121 tgcctgcgcg cggaaggtgg gcagcaggcg cttgatggaa tttacgatct cgatggtatt
     3181 ggtgcccggc tggcgctgga tggcgagcac gatgccgcgc tcgtcgttga gccaggcggc
     3241 gagcttgtca ttttccacgc tatcgatcac gcggccgatc tcgttcagcc gcaccggtgc
     3301 gccattgcgg taagcaatca cgaccgggcg aaaagccgcc gcattcatca gctgcccatt
     3361 tgactcaacc gtgtaggctc gattggcccc atagagcgtg ccggtgggca gattggtgtt
     3421 gtgatggttg atcgcttcgg cgacttcgtc gatgccgata ccgcgcgatg caagcgcctt
     3481 tggatcgagc tggatgcgca cagcgtattt ctgcgacccg aggacttgca cctgggccac
     3541 gccagagatc gtcgaaatgc gctgcgcaag cagcgtctgt gcgtactcat ccacggtcga
     3601 catcggcagc gtcgtggacg tcagcgccag gtagaaaatc ggcgcgtccg ccggattgac
     3661 tttttggaac gcgggcggcg tggtcatctg cggcggcaat tgcttcaatg cgcgcgtgat
     3721 agcagcctgc acgtcggcgg cagccgcatc gatgttgcgg tcgagcgaaa actgcagcgt
     3781 gacacggctg atgccctggg cgttggtgga gtccatcgaa tccagcccgg caatggtcga
     3841 gaattccttt tcgagcggcg tggcgaccgc ggacgccatg gtttcgggac ttgctccggg
     3901 cagtgatgcg gtcaccgcga tggtcgggaa atcgatattc ggcaggtctg aaacgggcag
     3961 gagccggtag ctgacgatgc caaaggccag gatgccgatc atgaccaggc cggtcatgac
     4021 cggccggcgc acaaagattt cggcaatgct catgatgtct tctccggcgt cttggcactc
     4081 actttcgaac cggggacgat gcgcgacgca ccatccacga cgacggtttc accctgttgc
     4141 aatccctcgg cgacgacgct atccactcct tcggtgcgcg caacttttac cagtcgcaac
     4201 tcgacggtcg aatcctgctt gacgacatag acgtactgcc cattcggtcc ggtctgcagc
     4261 gcctgcgtcg gtaccacgag cgctttgtct tcatcgcgca gggtgaccca ggattcgaca
     4321 aactgtcccg gccaaagccg cttgtcttca ttggcgaaaa tgccacgcag cttgatcgtg
     4381 ccggtgctca cgtcgactgc gttgtcgatg aaggccagct tgccgttgat ggtttcccct
     4441 ttttccactt cgacttgtac gcgcaacgct gctttcttgt ggtgctcgcg aatctcgttc
     4501 agatattgct ccggaaccgc gacgctcacg tagatcggcg tgagctggtt gatcaccacc
     4561 aacggcaacg tgtcgtttgc cttgaccatg ttgcctactg acaacatgac cttgccggcg
     4621 cggccgtcaa ttggcgagcg gatcgtcgtg tagccaaggt cgatacgcgc gtgctggacc
     4681 gctgcttcat ccgcgacaac gctggcttgc gcgccatgaa agttggcagc aacctgggca
     4741 taaccttcct tggagacaaa gttctgcttc agcagatcct ggtagcgcag ttcctgcccg
     4801 cgcaagtact cgagctgtgc ctggtcgcgc gcgaggttgg cctgggcctg ctcaagctgc
     4861 gactgat
//
LOCUS       DATMZQ010000540        11658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1676004, whole genome shotgun sequence.
ACCESSION   DATMZQ010000540 DATMZQ010000000
VERSION     DATMZQ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676004"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VN496_17285"
     CDS             complement(<1..216)
                     /locus_tag="VN496_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18764.1"
                     /translation="MVSIRDKGYIRAPLFLALVACICILAACGSGGGNDNGTTLSSAQ
                     SAALPTGAQAQSTKFIPTFLVYYGGGPA"
     gene            834..1925
                     /locus_tag="VN496_17290"
     CDS             834..1925
                     /locus_tag="VN496_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF18765.1"
                     /translation="MLIVDDGNTVNACREILAQLDLNIAHVSTQQAAMALMAERDFAV
                     ILLNVQMPNMDGFATARFIRRCERSRYTPIIFLTATYDDTVSGFRGYEVGGVDYILKP
                     LVPEILRSKIAVFVDLYRYNAKLGKEISDRKLIEDNLRKSEERLREFTAHMQSVREEE
                     RTSIAREIHDELGQALTALRMDLSWLEKRLPKEPPEPMEKVKSMFTLIDNTIQSVRKI
                     SAELRPQVLDDIGLTGTLKWQAREFQARTGIRCKVELPEKEFVLDLDRSTAIFRIFQE
                     VMTNVARHAKATRVDIKLRIDADRLVLHVADNGAGISESHLRSSRSLGLLGIRERATI
                     LGGDVQIQGVARKGTSVLLTVPLELLTKP"
     gene            1980..2612
                     /locus_tag="VN496_17295"
     CDS             1980..2612
                     /locus_tag="VN496_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF18766.1"
                     /translation="MMNVIIADDHTVVRQGLKQILAAEADMLVVGEAKNFTETLKLVR
                     TLDWDVLILDYSMPGGNGLQVLKEIKQSFPQRPVLILSMHPEDAIAISALRAGAAGYM
                     NKECASEELTTAIRKAVSGKKYVSASLAEKLAVELEEGSEGLPHELLSDREYRVMWMI
                     ASGKSISNIADELTLSPNTVSTYRIRIFKKLKVENNSDLVRYAIKHGLVE"
     gene            2920..5466
                     /locus_tag="VN496_17300"
     CDS             2920..5466
                     /locus_tag="VN496_17300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXF18767.1"
                     /translation="MTGAKSQQKPRASASVEAEIFAALEALRHGNFGYRMTLDIDGPG
                     HKVAQLFNDIMEMNVSMAKELVRIRDQVSAQQTNPQKINIPGAAGSWVVCVNAINVLV
                     ERLGSRMPNSLTQNQQAVLQQTNEKLKEQASKLFEQMQELEYKNKQIESARMALEQKA
                     EQLILSSRYKSEFLANMSHELRTPLNSLLILAQLLAENASGNLTPKQVEYAQTICISG
                     NDLLALINDILDLSKIESGTVTLGVANESLIELRDYVERAFRQVAHEKGLAFEIAMEK
                     ALPTLIRTDMKRLRQILQNLLSNAFKFTPQGTVSLDMSVADDGWTPGLLSLDCAENVI
                     AFCVSDTGIGIAQDKQRLIFEAFRQADGTTSRQFEGTGLGLSISAELARLLGGEIKVH
                     STPGTGSSFTLYLPQVFARVDLVRESEQPETQPGTQSGAVVPGSITARVSSAADEIDD
                     RENVRPGDRVVLIVMDEKLFAEKLLHHVRESGCRGLIAPNPHTALAMANEYMPNIVIV
                     DITSTGMSGWATRNLLKQDPDTRHIPVNMIALDERLDGCASMCAFGIVSKPSGRETLQ
                     EALGAARRSMGREVRNVLVAAASKEKRQSMMEAIAQEGVRINSASTGKQALKILRKAT
                     LDCAIIGDGLADMSGFIREFVQAGIEQTYLIICTAIHSVEGTEQVGQIAEIFRLKCEQ
                     SAGKVLGQTARCLQQALREMPPGHRRPPLSLRKTVSALAGRKVLVVDDDIRNIFAITG
                     ALEQQGMRVLNAGSGREGIELLKSNPDVDIILMDIMMPEQDGYETIRAIRGLDAADSV
                     PIIGVTAKAMMGDREKCIEAGATDYIAKPINIEQLLSLLRMWIADHSLES"
     gene            6254..7450
                     /locus_tag="VN496_17305"
     CDS             6254..7450
                     /locus_tag="VN496_17305"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HXF18768.1"
                     /translation="MKRFESYAKGLTWSAAFVLTAFLAGCGGGDDGGGGFVGGNNAAP
                     TVLSTAPADAAASVCVNQAANATFSKAMDPASVNDTSFTVADSTGAAVPGTVAMDSTK
                     KIATFTPAADLQPSTAYTATINGGANGVKDSGGKTLAANKVWTFTTDDGTCRVQAPVA
                     LGNASTFGAASGVGITNTGTLTKITGDAGVPAASTSVTGLHDMTPPPGVVFTEVPGTN
                     VGEVSGTIFTTDSPAADAGQTVQAMASDVSAAFDDLAARPGGTDPSNGTGELGGLTLG
                     PGVYLANTFQVTGGDLTLDAQGDPNAIWVFQCDTDGPTIGDTAARSVTLKNGAQAKNV
                     YWASNGAATINSVGGGTMVGTIITRNGASIGSGTSTGTTNVKGRVFVLNNPITMNNAV
                     ITVPAP"
     gene            7929..8606
                     /locus_tag="VN496_17310"
     CDS             7929..8606
                     /locus_tag="VN496_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXF18769.1"
                     /translation="MRVLLADDHQIVRQGLKGILEKAGHVVVGEAADGHEALKLARTL
                     NPEIAVLDLSMPLLNGLDTAREIHSLSPEIKTIILTMYTDKGYVLRALKVGAKGYVLK
                     SQAADDLIRAIREISRGEVYLSPGVAASVVDAYLEKTGAAADPLTPRERQVLQLIAEG
                     STTKEAAKYLNISFKTAETHRNHIMKKLNIHDVVGLVRYAIEKGLVHVDVHSDFWNTQ
                     NRRAAPW"
     gene            complement(8741..9358)
                     /locus_tag="VN496_17315"
     CDS             complement(8741..9358)
                     /locus_tag="VN496_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18770.1"
                     /translation="MRNFANPSIARCLLVLLVLVSAGTAAQDHAPIRLRGTVEKFVEP
                     SLVIRERSGKTFTMTLPEETGIVEVIPTDITSVQPGAYIGTAAVARADGKLVSLEVVI
                     FPESARGTGEGHFPWDLQPESSMTNATVAELSRSSDGRTLKLRYKDGEKTVVVPDGVP
                     VVTLRPGNRSFIVPGAKLFAVVEPVDDGYIVRRLLVGRNGVAPPM"
     gene            complement(9383..10195)
                     /locus_tag="VN496_17320"
     CDS             complement(9383..10195)
                     /locus_tag="VN496_17320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXF18771.1"
                     /translation="MHPMLNIAVKAARRAGTIISRASENLDVLTIKHKSLNDLVSEVD
                     RAAEDAIIDVIGSAYPEHAILAEESGASGQSEYVWVIDPLDGTTNFLHGFPQYCVSIG
                     LLHKGIPSQAVIFDPTRNDLYTASRGRGAFLNDKRLRVAKRDKLIDGLIGTGFPFRMF
                     DYLESYNAMLKDLMTKCAGVRRPGAAALDLAAVAAGRFDGFWEIGLKPWDMAAGVLMI
                     TEAGGLVADFEGNDTFMERGQIVAGSPKIFSQLLQLIQPHLTAEMKSLSKVL"
     gene            10258..10986
                     /locus_tag="VN496_17325"
     CDS             10258..10986
                     /locus_tag="VN496_17325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXF18772.1"
                     /translation="MKRLSVLGQIRIVLSHTTHPGNIGAAARAMKTMGLSRLFLIKPE
                     RFPHPDAQTFATGASDILSNAVVCGDLNEALSGTVLAIASSARHRDLAHEIVDCREGC
                     RRLLAQAETGDVALVFGTERAGLTVDEVSRCQSIAVIPTDTEYSSLNLAQAVQVFAYE
                     LRMCALGEQQYLQHAAEPATHEETEGFFRHLEETFYSTGFLDPKEPKRLMQRMRRLFA
                     RARIEKKELNILRGFLSAIKSKVE"
     gene            11023..>11658
                     /gene="cysE"
                     /locus_tag="VN496_17330"
     CDS             11023..>11658
                     /gene="cysE"
                     /locus_tag="VN496_17330"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HXF18773.1"
                     /translation="MFDRASEEIRVIFDRDPAARSTWEVITCYPGFHALLFHRFSHRL
                     WRWNLKWLARFISHIARWLTGIEIHPGATIGRRFFIDHGMGVVIGETATIGDDCTLYH
                     GVTLGGTSWNKGKRHPSLGEGVVVGAGAKILGPITVGRGAKIGSNAVVVKDVPDGATA
                     VGIPARILDDASSNAREESAAKMGFTAYGIGADVNDPMVKAIHGLIDHCAAA"
BASE COUNT         2872 a         2992 c         3226 g         2568 t
ORIGIN      
        1 cgcaggaccg cctccgtagt aaaccaggaa cgtcggtatg aatttggtgg actgcgcctg
       61 cgcacccgtc ggcaacgcgg cagactgtgc actggacaat gtcgttccat tgtcattccc
      121 gcccccactg ccgcacgcgg caaggatgca gatgcaggct accaacgcca ggaacaacgg
      181 cgcacgaata tagcctttgt cccggatgct aaccattggc gggaagatgc tttgaatttt
      241 gaaagaggaa ccggggcaac aggagatatg gaaaggacgg aaacagaaat tcatgttgat
      301 tcctcgcaac gtaagcgaac taaaagcaac gtaggagaac acctacatga ctgtagttgt
      361 tcgtcgcgga gtttatacga attaaagaaa aagctattgt tttatcccgt aatatagtgt
      421 cgtgacatga cgacactatt gtcgttgtcg atggctgcaa cgggaatcag aagtgccaga
      481 acgagatgcg ggaaagagcg cctctaagtg acttgtgcct ggcgctgagg cgtggattgc
      541 cggcgattac ggttcatcgc ttcgtcataa tttttcttgg cgtactaact gcagctggac
      601 aagcaacatt cgcgagcttt gggaacggcg ttgattggcg attgagcaaa ggacacgcta
      661 aagaacgtgg caacagcatt tatgatttgt cacataacag tgcgagatag cgattgtcgt
      721 cacgataccg agcgaagttc agcacacggt cctccgtgat attgttctgt ctcgcgacat
      781 cgcaatacga ccacacctgc atctaagcca tgtcccgccc ggaacacccc accgtgttga
      841 tcgtcgatga tggcaacact gtcaatgcgt gccgagaaat cctcgctcaa cttgacctga
      901 acatagcgca tgtatccacg cagcaagcag ccatggcgct catggcagag agagactttg
      961 cggttatttt gctgaatgtg cagatgccga atatggacgg cttcgcaact gcaaggttta
     1021 ttcgacgatg cgagcgctcg cgatacaccc ccatcatatt tttgaccgct acttatgacg
     1081 atacggtatc cggctttcga ggttacgaag tcggtggcgt agactacatt ttgaaacctt
     1141 tggtacccga aatactcaga tcaaagattg cagtgttcgt tgatttgtac agatacaacg
     1201 cgaagctggg caaggaaatt tcagaccgga agctcattga ggataatctt cgcaaatcgg
     1261 aagaaaggct cagggaattt acagcgcaca tgcaatccgt tcgtgaagaa gagcgaacaa
     1321 gtattgcgcg cgaaattcat gatgagctgg gacaggcatt gaccgccctg aggatggatc
     1381 tgtcatggct agagaaaaga ctgccgaaag agccgccgga gccgatggaa aaggttaaat
     1441 cgatgttcac tctgatcgac aatacaattc aatcggtgcg caagatctcc gccgaactga
     1501 ggccgcaagt gctggacgat atcggtctga caggtactct aaaatggcaa gctagggaat
     1561 ttcaggcgcg tacgggtatt cgatgcaaag tcgaattgcc cgagaaagaa ttcgtgctgg
     1621 acctggacag gtccactgct atattccgca ttttccagga agtcatgaca aacgtagcac
     1681 gtcatgcaaa agctaccaga gtggacataa aattgcggat agatgcggac cggttagtac
     1741 tgcacgtggc ggacaatggt gcaggcattt cagaatcgca cctgcgtagc tcaaggtctc
     1801 tcggactgtt aggtatccgt gagcgggcca ctatcctggg aggagatgtg caaatccagg
     1861 gtgtcgcccg taaggggaca tcagtgctcc ttaccgtgcc gcttgaactg ctaaccaagc
     1921 cctagctttt cacggtccgc aaagattatc ggtcaagcag cagaaaccag agagatggca
     1981 tgatgaacgt cattattgct gacgatcaca cagtggtgcg tcaaggcttg aagcagatcc
     2041 tcgctgcgga agcggacatg ctcgtcgtcg gagaagcgaa gaacttcact gaaaccctca
     2101 aactcgtacg tacgctggac tgggatgttt tgatactcga ttactcgatg cccggtggaa
     2161 acgggcttca agtgttgaaa gagatcaagc aatccttccc acagcggcct gtgctcatac
     2221 tgagcatgca tccggaagac gcgattgcaa tttcagcgtt gcgtgccggc gcggcaggtt
     2281 atatgaacaa ggaatgcgct tcagaagaat tgacaaccgc cataaggaag gcggttagtg
     2341 gtaaaaagta tgtcagtgca tcgctcgccg aaaaacttgc cgttgaactt gaagaaggtt
     2401 cggaaggcct tcctcatgaa ctactttcgg atcgcgagta tagggtaatg tggatgatcg
     2461 cttccgggaa atccatctcc aacattgccg acgaactaac ccttagcccg aacactgtca
     2521 gcacgtaccg gatccgcata ttcaagaaac tgaaagtcga aaacaattca gacctcgtcc
     2581 ggtacgcgat caagcacgga ctcgtagagt gacagcttta tagttttgct tactcttccg
     2641 ttgccgattt gtagaatatt tcctacggat ccctcagtgt tttcgtccta cggaaataag
     2701 tagtcagtaa gcagcttcgc tctgtttgct gggctagcct gcgtaattgc caatgcgaga
     2761 agctaaatca cgatggcttt gctgctggtc aaggcaggca attactgtat gcggcgctgt
     2821 ttttctcatt ctgtaataac cacgctagct tctcttccat gagtgctttc atcgttgagc
     2881 gctacgagct gttggagtca atagggaagg agccaccatt tgaccggcgc taagtctcaa
     2941 cagaaaccaa gagcaagtgc gtccgtagag gctgaaatct tcgcggcatt ggaggcgctc
     3001 aggcatggga acttcggata tcgcatgacc ttggatattg atggtcctgg tcacaaagtg
     3061 gcgcaactgt tcaatgacat catggaaatg aatgtttcga tggcaaaaga attagtgcgg
     3121 atccgcgatc aggtcagtgc gcagcaaacg aatcctcaaa aaataaatat acccggggct
     3181 gcaggctcgt gggttgtctg cgttaatgcg atcaacgtgc tggtcgaacg cctgggttcg
     3241 cggatgccga attcattgac ccagaatcaa caggccgttc ttcaacaaac caacgaaaag
     3301 ctcaaggaac aagccagcaa gctcttcgag caaatgcagg aactggaata caagaacaag
     3361 cagatagagt ccgcccgaat ggcgctcgaa caaaaagcag aacagctgat attgagttcg
     3421 cgatacaagt ccgaattcct tgccaacatg tctcatgaac tgcgcacacc attgaacagc
     3481 ctgctaatcc tggcgcagct gctggccgaa aatgccagcg gcaatctgac cccaaagcaa
     3541 gtcgaatacg cgcaaaccat ttgtatctcg ggaaacgatc tgctggcgct gatcaatgac
     3601 atcctcgact tgtccaagat tgaatctgga accgttaccc tcggcgttgc aaacgaaagc
     3661 cttattgaat tgcgtgacta cgttgagcgg gcgtttcggc aggtcgctca cgaaaaaggc
     3721 ttggcgtttg agatagcgat ggaaaaggca ctaccgacct tgattcgcac cgacatgaaa
     3781 aggttgcgcc agattcttca aaacttgttg tcgaatgcct tcaaatttac accgcagggc
     3841 accgtgtcgc ttgacatgtc ggtggccgat gacgggtgga cgcctgggct tctcagcctg
     3901 gattgcgccg agaacgtgat cgcgttctgc gtgagcgata ccggaattgg cattgcccag
     3961 gacaagcagc gactcatttt cgaagcattc cggcaggcgg atggaacaac cagtcgccag
     4021 ttcgaaggta caggtctggg gctgtcgatt agcgcggaac tggcccgtct gctgggcggg
     4081 gaaatcaagg ttcatagcac tcctggaacg ggtagtagtt tcacgctcta tctgcctcaa
     4141 gtcttcgcgc gagttgatct tgtcagggaa agtgaacagc ctgaaacgca gccaggaaca
     4201 caaagcggtg ctgttgtccc tgggagcata acggcaaggg tttcgtcagc cgcggatgag
     4261 atagacgacc gcgaaaacgt ccgtcccggg gaccgggtag tgctgatagt tatggacgag
     4321 aaattgtttg cagagaagct tctgcatcat gtgcgggaat cggggtgcag aggattgatt
     4381 gcccccaatc cccacacggc gctcgctatg gcaaatgaat atatgccgaa catcgttatt
     4441 gttgacatta cgtcaacggg catgagcggc tgggcgaccc gtaatctgct caagcaggac
     4501 ccggatacca gacacattcc agtaaatatg attgcactcg acgaacgcct ggatggctgc
     4561 gcgtccatgt gcgcgtttgg aattgtgagc aaaccctctg gccgtgagac gttgcaggag
     4621 gcgttaggcg cagcgcgccg atccatgggg cgggaagtcc gcaatgtcct tgtcgctgct
     4681 gcaagcaagg agaagcggca aagtatgatg gaagccatcg cgcaagaagg agtgcggatc
     4741 aatagcgcgt cgacgggcaa gcaggcgctg aagattttgc gcaaagccac cctcgattgc
     4801 gcaattattg gtgacggctt ggctgacatg tcagggttca ttcgagagtt tgttcaggcg
     4861 gggatcgaac agacctacct catcatttgt accgcgatac attctgtcga aggcaccgag
     4921 caggttggac aaattgccga gatttttcgg ctgaagtgcg aacaatctgc tggaaaggtt
     4981 ctgggacaaa cggctcggtg cctgcagcag gcgttgaggg aaatgccgcc cggacatcga
     5041 cggcccccct taagcctccg caagacggtt tctgctctgg caggccggaa agttctagtc
     5101 gtcgatgatg acatccgcaa catatttgcc attaccggcg ctctggaaca acagggaatg
     5161 cgggtattga atgccgggag cggcagagag ggcattgaac tgctcaagtc gaatcccgac
     5221 gtggacatta ttctgatgga cataatgatg cctgaacagg atggctacga aaccattcgc
     5281 gctatccggg gattggacgc tgcagattct gtcccgatta ttggagtcac cgccaaggca
     5341 atgatgggcg accgcgaaaa atgtatcgaa gcaggagcga ccgactacat cgccaaacca
     5401 attaacattg agcaactcct gtcattgcta cggatgtgga tcgcagacca ttccttggag
     5461 tcttgacgtg tagaaccgat gcatgaccca tgcggtactg aggtgaatcg ggcattttgc
     5521 ggtaccagaa ataattaagt ccatctggcg gcgtttgatc tgccgcgcat ttccaatgcc
     5581 agcaatgttg ctgttcattg gtgaaaaaag ttctccagga ccgtggccca ctggtcccac
     5641 tttcttggat ttgtccccgc gtcgtctcgc gccggcaccg catttgaaaa cgaccgttcc
     5701 tagagcggaa tccaacccag gaacgatccg acaccgacaa cacagagcca gattatcgcg
     5761 atgcccgcta tccataggac ctgcggaacg gacttctgaa attcgaatct cattactgca
     5821 ccttcttcgg gtctcgtgaa atattatgca aaagcgcttt gttgcgacga ggcgtggcat
     5881 ttcctcatag tggaacggta gcgtcgcaaa aaactgaagt ccgtgcgtcc gcgcacataa
     5941 cgcaatgcgc tttttactct tcagacacag ggttcaggac atcagcccat ttgcaaaatg
     6001 caaacgaatg gctggtcgcg agacttcaaa cccgaatgtc attgccgata gtccgattgg
     6061 accaagacgg aaagtccctg ataaatgtca tccaattatt ctgcctcgac gtcggataac
     6121 ggacggtggc aaacgcaatt agccatgcgc ccacattccg gctcggtttt gtaatgccag
     6181 tgaattatcg gggcagcgct gatattcagc ggcaagtgcc atgagcgacg cggacaatga
     6241 ggaaggaaag cacatgaaaa gatttgagag ttacgcaaaa ggattgacat ggtcagctgc
     6301 gtttgtgctg accgcttttt tggccggatg cggcggtgga gatgatggcg gcggagggtt
     6361 tgttggaggc aacaacgcgg caccgacggt actttcaacc gctccggccg acgcggctgc
     6421 aagcgtttgt gtgaaccagg ccgccaatgc gactttcagc aaggcaatgg atccggcgtc
     6481 tgtaaacgac acaagcttta cggtcgctga ttcaacgggc gcggcggtgc cgggaacggt
     6541 tgccatggat tctacaaaaa agattgcaac cttcacgccc gcagcagact tgcagccgag
     6601 cactgcctac accgccacaa tcaatggcgg cgcaaacggg gtcaaggatt ccggaggcaa
     6661 aacattggcg gcaaacaagg tctggacctt caccaccgac gacggaactt gcagagtgca
     6721 agcgccagtg gctcttggta atgcatcgac ttttggcgca gccagtgggg tcgggataac
     6781 caacaccggt acattgacga agattaccgg agatgcaggc gtcccggctg cgtcgacgtc
     6841 cgtcacgggt ctgcatgata tgacaccgcc gcctggagtg gtgtttaccg aagtgccagg
     6901 tacgaacgtt ggcgaagtta gcgggacaat ctttacaacg gatagtcctg ccgccgatgc
     6961 tggtcagacc gtccaagcca tggcatccga cgtgagcgcc gcatttgatg atctggctgc
     7021 ccgacctggc ggtactgatc cgtcaaacgg gaccggtgaa ctgggcgggc tgacactcgg
     7081 gcccggagtc tatctggcaa atacgttcca ggttactggt ggcgatctta cgctcgatgc
     7141 acaaggtgat ccgaatgcga tttgggtctt ccagtgcgac accgacggtc ctaccatcgg
     7201 cgacaccgca gcccgtagtg tgacactgaa aaatggggct caggccaaga acgtgtactg
     7261 ggcttcgaat ggtgccgcga ctatcaattc ggttggcgga ggtacgatgg taggaaccat
     7321 cattaccaga aacggcgcca gcattggttc cggtaccagc accggcacga cgaatgtcaa
     7381 gggcagggtg tttgtcctta ataatccgat cacgatgaac aacgcggtga tcaccgtccc
     7441 ggctccgtaa ggaagtgatg tccgttgtcg aaattcggca acggacattt ggagtcccat
     7501 ccaaggcgga aacaaaaagg gcccgaacca tgggtccttt ttgtatggaa tgtctatcga
     7561 gcatacatgc ctgtcagttt gcgctttggg tcagtttcag ataagtacca acttcattcc
     7621 tcccgatacc atgcaccaag attgcacttt tgatctgaac ggacgttatg ttaagcatcg
     7681 aacagaactt aagtggtgtc aggagcagca tttgtgacgg tgtgcgcgga tttgaaagcg
     7741 atggcggtac aattcagcgc tgtgacggat acgcgcctta acgaagcaag catggccccg
     7801 tgacattcaa aggggtgcag ccaactggat tgccggactg agcagttccc cgtgctggtc
     7861 cgttcgccga aacaccgaac ctttgaatgg aatgaccatt caaagagata tcgtttttgg
     7921 aggctgacat gcgggtactt ttggctgatg accaccagat tgtccgacaa ggattgaaag
     7981 ggatcctgga aaaggctgga cacgtggtcg tcggcgaagc ggctgatggt cacgaagcgc
     8041 tcaaactggc acgcacgctc aatcctgaaa tcgcagtgct cgatctttcc atgccgctac
     8101 tgaacggttt ggatacggca cgggaaattc acagtctttc gcccgagata aagaccatta
     8161 ttcttactat gtataccgac aaaggatatg tgctcagggc attgaaagtc ggtgcgaaag
     8221 gttatgtcct caaatcccag gctgctgatg atcttattcg ggccattcga gaaatatcac
     8281 gcggtgaggt ctatttgagt cccggggtgg ccgcatcagt cgtcgatgcc tatctcgaaa
     8341 aaaccggcgc ggcagccgat ccccttacac cgcgcgagcg ccaggtgctc cagcttatcg
     8401 ccgaaggaag tacaaccaaa gaggccgcaa agtatttgaa tatcagtttc aaaactgccg
     8461 aaactcatcg aaaccacatc atgaagaaac ttaatatcca cgatgtggtt ggcctggtgc
     8521 gttatgcgat tgaaaaaggg ctggtgcatg tcgatgtgca cagcgatttc tggaatacgc
     8581 aaaaccgcag agccgctccc tggtgaacac ggggacaggg acaggcgtgg tctctattgt
     8641 cccttcagcc ggtaatggat cagccgctgc ttccgcggcc accatcttaa tgcgaccggc
     8701 caacttgcaa gcgtgtttgc actccgaccg gggtcaccgg ttacatcggg ggcgctacgc
     8761 cattgcggcc gaccagcaag cgtctcacga tgtatccatc gtcaaccggc tcaacgacgg
     8821 cgaacaattt cgcaccgggt acgataaacg aacgattacc tggcctcaac gtaacgaccg
     8881 gcacaccgtc gggaacaacg acagttttct caccgtcttt gtaacgcagc tttaacgtac
     8941 gaccgtcgga tgatcttgat agctccgcaa ctgtggcgtt cgtcattgaa ctttcgggtt
     9001 gcaaatccca tggaaaatga ccttcaccgg ttccgcgcgc ggattcaggg aaaatcacca
     9061 cctcgagcga taccagtttg ccatcggcgc gcgcgaccgc cgcggtacca atatatgcac
     9121 cgggctgcac cgatgtgatg tcggtcggta taacttcgac tatgccggtt tcctccggca
     9181 gcgtcatagt gaaagttttg ccgcttcgct cgcgtatcac caggctcggt tcaacgaact
     9241 tctcgacagt accgcgcaag cgtatcggcg catggtcttg cgccgcggtg ccggcggaaa
     9301 ccagcaccaa caaaaccagc aggcaacgtg caatggacgg gtttgcgaag ttcctcatag
     9361 aaattcaaga gcgcttcgga tctcacagca ctttcgataa cgatttcatt tcagcggtca
     9421 ggtgtggttg aatgagctgc aatagctgcg agaatatttt cgggctgcca gcaacgattt
     9481 gcccgcgctc catgaacgtg tcgttgccct caaaatccgc aaccagaccg cctgcctcgg
     9541 tgatcatcag gacaccggcc gccatatccc acggtttcaa cccgatttcc caaaatccgt
     9601 cgaagcgccc ggccgccacg gctgcgagat ccagcgctgc cgcaccgggc cggcgcactc
     9661 ccgcacattt ggtcatgagg tctttcagca ttgcgttgta cgattcgagg taatcaaaca
     9721 tccggaacgg aaagccggtg ccgatcaggc cgtctatcaa tttgtcccgt ttcgctactc
     9781 gcaacctttt atcattcagg aatgcaccgc ggccacgact ggcggtataa aggtcattgc
     9841 gcgtcggatc aaaaataaca gcttgtgacg ggataccctt gtgcagcagc ccgatggata
     9901 cgcaatactg cggaaagcca tgcaggaagt tggtagtgcc atcgagcgga tcgatgaccc
     9961 agacatattc cgactgcccg gacgcgccgc tctcctctgc taaaatcgca tgctccggat
    10021 aggcgctacc aatcacatcg atgatggcgt cttccgcggc gcgatccact tccgaaacca
    10081 ggtcattgag gctcttgtgc ttgatcgtca gtacgtccag gttttccgaa gcccggctga
    10141 tgatcgtgcc ggcacggcga gcggctttca ctgcgatgtt cagcatcgga tgcatggcgg
    10201 cgaaatggtg ctaaagaacg aaggcgccat tctagagatt ccgcccgttt cattccaatg
    10261 aaaagactct cggtgcttgg ccaaatccgt atcgtccttt cacataccac tcatcccggc
    10321 aacatcggcg cggctgcgcg agcaatgaag accatgggcc tttccagact gttcctgatc
    10381 aagccggaac gttttccaca tccggacgcg cagacatttg ccaccggagc aagtgacata
    10441 ctctcgaacg ctgttgtatg cggcgatctg aacgaggccc tttcaggaac cgtgctcgca
    10501 atcgcgtctt cggcacggca ccgtgatctt gctcacgaaa tagtggattg ccgggaaggg
    10561 tgtcgacgac tattggcaca agcggagact ggggatgtcg cgttggtatt cggcactgag
    10621 cgtgctgggc tgaccgtgga cgaagtcagt cgatgccaat ccattgccgt cattcccact
    10681 gatacggaat attcttcgct caatcttgcg caggccgtac aggtgtttgc ttatgagctg
    10741 cgtatgtgtg cgctgggcga acagcagtac ttgcagcatg ccgccgaacc ggcaacgcat
    10801 gaagaaaccg aaggattttt ccggcatctt gaagagacgt tttactcgac cggctttctg
    10861 gacccaaagg aaccaaagcg tctgatgcag cgtatgcgcc gtctgtttgc gcgtgcccgc
    10921 attgaaaaga aggaattaaa catcctgcgt ggtttcctga gcgccataaa gtccaaagtt
    10981 gagtaaaata ctcatcttcc aacctacagt aataaaaggg ttatgttcga ccgggcgagt
    11041 gaagagataa gggttatttt cgatcgggat ccggctgcac ggtctacgtg ggaagtcata
    11101 acctgctatc cggggttcca cgcgcttctg ttccaccgtt tttcacatcg gttatggcgc
    11161 tggaacctga aatggctggc gcgttttatt tctcacattg cgcgctggtt aaccgggata
    11221 gaaattcatc ctggcgccac gataggacgc cgttttttca tcgatcatgg tatgggcgta
    11281 gtgatcgggg agaccgccac gatcggtgac gattgcacgc tgtatcacgg tgttacgctt
    11341 ggaggcacgt catggaacaa gggcaaacgt catccgagcc tgggcgaagg tgtggtggtt
    11401 ggggccggcg ccaagatcct tggtccgatt accgtggggc gaggcgccaa aattggttca
    11461 aatgcagttg tggttaaaga cgtacccgac ggcgccacag ccgtcggcat acctgcgcgg
    11521 attctggacg acgcatccag caatgcgcgt gaggaaagtg cggcgaagat gggtttcact
    11581 gcatacggaa ttggtgcgga cgtgaacgac cccatggtca aggcgattca cggccttatt
    11641 gatcactgcg ctgccgct
//
LOCUS       DATMZQ010000541        11449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1703783, whole genome shotgun sequence.
ACCESSION   DATMZQ010000541 DATMZQ010000000
VERSION     DATMZQ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703783"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..746)
                     /locus_tag="VN496_17335"
     CDS             complement(18..746)
                     /locus_tag="VN496_17335"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXF18774.1"
                     /translation="MTEFSPTELLVTVIARLLHGCAHVAVGASSPIPGAGALLARSHS
                     GSSMRVSVLGSIKDNFFTSGGVELFDLAAQGRIDAFFLGGGQIDGQANINLVGAGPYP
                     NNRVRWPGSFGSAYLYFLVPRVILFREEHTRRVMVPKVDFISAPGVSEPGVYRPGGPY
                     ALLTGLALFSFDRAQRRFRLDSVHPGHSLEEVRDNTGFEFDCPPDVPVTRKPDAAVLA
                     AIRGRIREDIARTYPQFAARLSPA"
     gene            complement(743..1567)
                     /locus_tag="VN496_17340"
     CDS             complement(743..1567)
                     /locus_tag="VN496_17340"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXF18775.1"
                     /translation="MNAPSLTSLETLVEAIPDGALLAVPKDSSGVAMAATRALLGRKV
                     HDLHLLCVPVGGLQADLLIGTGAVSVVETSAITLGEFGTAPCFTQALHSASLRILDAT
                     CPAIYAALQAGEKNIPFIPLRGMIGTDLLRHRSDWKVIDNPFSPGDPIVLLPSIRPDV
                     ALFHARYADREGNIFIGKEREVLLMAHAAKTTLVTVEEIVDGNLLDDPARGGAVLPSI
                     YVSAIAVAKHGAWPLALSDLYAMDDAAIARYITLARTVEGLEQFIGQWDSGVQAAA"
     gene            complement(1636..2193)
                     /locus_tag="VN496_17345"
     CDS             complement(1636..2193)
                     /locus_tag="VN496_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HXF18776.1"
                     /translation="MALKATVFKAALHIADMERGYYHDHALTIARHPSETDERMMVRL
                     LAFALNADEALSFGAGLSTDDEPDLWRKDLTGNIETWIDVGLPDEKRMRKASGRAGHV
                     LVYTYGGRAADLWWQQVSDKLARLNKLSVINLPHAGTQALGRLAARNMDLQYTMQDGQ
                     VWVADGHQSVQIDPQVMLSAKRREH"
     gene            complement(2302..4356)
                     /locus_tag="VN496_17350"
     CDS             complement(2302..4356)
                     /locus_tag="VN496_17350"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXF18777.1"
                     /translation="MSSALIFALVCAIIGIAYGAISIKWILGQPAGNARMQEIAAAIQ
                     QGAQAYLNRQYATISMVGVVLFVLLGFGLDWYSAIGFLIGAVLSGLTGFIGMNVSVRA
                     NVRTAEAANNGINAALQVAFRGGAITGMLVVGLGLLGVAGYYGILLALVEPDKALHSL
                     VGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNV
                     GDCAGMAADLFETYAVTIVATMLLGGLLVGQVGPHAVLYPLVLGGASIIASIIGTFFV
                     KAKEGGKIMNALYKGVIVSGVLAAIAFYFITGRMMGDSPLGAMNLFYASLVGLVLTAL
                     MVVITEYYTATEYGPVRHVAAASQTGHATNIIAGLGVSMKSTALPVLAVCAAIWASYL
                     FAGLYGIAIAATAMLSMTGMIVALDAYGPITDNAGGIAEMAGLPAKIRDITDPLDAVG
                     NTTKAVTKGYAIGSAGLAALVLFADYTHNLESLHKLGAGALVKGFELSDPAVIIGLFI
                     GGMVPYLFGAMAMEAVGRAAGAVVNEVRRQFRDIKGIMEGKAKPDYSRAVDMLTKAAI
                     KEMLIPSLLPVLVPVIVAFGMNWLMGPGAGIRALGGLLIGTIVTGLFVAISMTTGGGA
                     WDNAKKYIEDGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLLVP
                     LL"
     gene            complement(4519..5775)
                     /locus_tag="VN496_17355"
     CDS             complement(4519..5775)
                     /locus_tag="VN496_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXF18778.1"
                     /translation="MKMNAFYAQSGGVTAVINASAAGVIETARRHKNKIGKVFAGRNG
                     IIGALTEDLIDTSKESATAIAKLRHTPAGAFGSCRYKLKGLEENRAQYERLIEVFKAH
                     DIGYFFYNGGGDSADTCLKVSGLSESLGYPLQAIHVPKTVDNDLPVTDCCPGFGSVAK
                     YIAVSMREASFDVASMAKTSTKVFILEVMGRHAGWIAAAGGLAADRPGDLPIMILFPE
                     VPFEKVAFLARVQALVKQYGYCSVVVSEGVRDSSGGFLADQGLRDAFGHAQLGGVAPV
                     VAAVVKEGLALKYHWAVADYLQRSARHIASKTDVAQAYAVGKAAVELAVKGRNAVMPT
                     IVRKSNKPYKWTVGVANLSDVANVEKQLPRDFITEDGYGITAKCRQYLSPLIQGEDYP
                     PFGNGLPRYVTLKNTAVARKLAEFKV"
     gene            complement(5859..6512)
                     /gene="adk"
                     /locus_tag="VN496_17360"
     CDS             complement(5859..6512)
                     /gene="adk"
                     /locus_tag="VN496_17360"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXF18779.1"
                     /translation="MRLILLGAPGAGKGTQAAFITGKFGIPQISTGDMLRAAVNAGTP
                     LGLKAKKFMDAGALVPDDIIIGLVKERIKQPDAAKGFLFDGFPRTIPQADAMKNAGVM
                     LDWIVEVDVDDAEIIRRLSGRRVHPASGRTYHVIFNPPKVAGKDDLTGEALVQRADDT
                     EETIKKRLEVYRSQTMPLIAYYSNWASSGTPGAPKYVKVAGVGSMEAIRDRIFSAVK"
     gene            complement(6598..7362)
                     /gene="kdsB"
                     /locus_tag="VN496_17365"
     CDS             complement(6598..7362)
                     /gene="kdsB"
                     /locus_tag="VN496_17365"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXF18780.1"
                     /translation="MRFVVVIPARYASARLPGKPLADIAGKPMVVHVADRARESGAHE
                     VIVATDDQRIQQAVAKHGHQALMTRPDHPSGTDRIAEIAAVRGWPDELIVVNAQGDEP
                     RMPSQLLRSVAEQLAAHSDAAIATACHRVHAASEMFDTNAVKVVLDQQGYAIYFSRAP
                     IPYARDAFKTSTSNLPRGLPAYRHLGLYAYRCSFLKIYSTLAPAAIEQFESLEQLRAL
                     WHGYRISVVVTDQAPDPGVDTPEDLENMRRVFQSGR"
     gene            complement(7433..7615)
                     /locus_tag="VN496_17370"
     CDS             complement(7433..7615)
                     /locus_tag="VN496_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXF18781.1"
                     /translation="MDPKLLDILVCPLCKGPLLYKKAEKELVCKACRLGYPIVDGIPV
                     MLEDEARKLPPEEEVR"
     gene            complement(7605..8600)
                     /gene="lpxK"
                     /locus_tag="VN496_17375"
     CDS             complement(7605..8600)
                     /gene="lpxK"
                     /locus_tag="VN496_17375"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXF18782.1"
                     /translation="MAFLDRHWYRLSWLSILLLPFAALFACVAAIRRLLYGSGLLHSY
                     RLAVPVIVVGNISVGGTGKTPLVLWLCDFLTEQGYAPGIVSRGYGGGGQIMAVMPSSP
                     PEIVGDEPVLLAMRSRRPVWIGRNRAAAARALIAAHPECNVIVSDDGLQHYAMARDFE
                     IAVIDGSRGTGNGLPLPAGPLREGTRRLNRVDAVVITGNALAPVAGKPNFGMALEGTM
                     FRNLLNPAFHQDAAAFQGKHVHAIAGIGNPGRFFDQLQRMTLNFVAHPFPDHHAYSAS
                     DLEFANADAIIMTEKDAIKCLPFARENHWVLPVDAVVDTDLGRLIVEKLKNRHGS"
     gene            complement(8675..9094)
                     /locus_tag="VN496_17380"
     CDS             complement(8675..9094)
                     /locus_tag="VN496_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXF18783.1"
                     /translation="MNFRRGSFREEPEINLVPFIDILIVIVIFLAVTTTYSRYSEMQI
                     NLPTADASKPADRPNQINVGVSSNGQYMINRAAVGFTTPEALATELKRAAGSNTDPVI
                     IINADANATHQSVINVMEAARVAGFSKITFTTQQQRR"
     gene            complement(9106..9717)
                     /locus_tag="VN496_17385"
     CDS             complement(9106..9717)
                     /locus_tag="VN496_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXF18784.1"
                     /translation="MLHIIEAAGWPIWPLILCSIITLGIIGERFYTLRKDFVAPRDLL
                     PRVVQEYRQKGVSADMLDRLAQGAMLGRIFAAALKEAASSREIMKESIEEAGRAVANE
                     LGRFLTTLGTIATMAPLLGLLGTIIGMIEIFGSQGPTGMANPGQLAHGISIALYNAAF
                     GIIVAVPSLVFYRHFRARADSLVVEMEVQAVKLVEIIHGQRQA"
     gene            10152..>11449
                     /gene="xseA"
                     /locus_tag="VN496_17390"
     CDS             10152..>11449
                     /gene="xseA"
                     /locus_tag="VN496_17390"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXF18785.1"
                     /translation="MHIDDANVAVISVAELNRRAREFIETGFPLLWIAGEICNFTRAA
                     SGHCYFSLKDATAQVRCVFFRNKAALVGWKLENGMQVEVRALPTLYEARGDFQLTIEG
                     MRRSGLGALFEAFERLKLKLEQEGLFDALRRKPIPQFPAAVGVVTSLTGAVLHDVLTT
                     LGRRMPSIRVVVYPAPVQGEGAGTRIARAIRMASMRREVDVLIICRGGGSMEDLWAFN
                     EEIVARAIVECGIPVVSGIGHETDFTITDFVADARAPTPTAAAELVSPNRFELLARVG
                     VLGKRLSRDIHRALNTRMQQIDYLTKRLMHPGERMQAELRHLAHLANRMHAASRRTID
                     AAAWNLSDLSRHLRGTLPDVDALIEKQQALQQRLRFSQARVLEQHAASLGKAKANLEH
                     LNPHAVLDRGYSIVQKNDGRIVGAAQQIATGELLRLTFASG"
BASE COUNT         2363 a         3373 c         3394 g         2319 t
ORIGIN      
        1 agtgggtcac tgatggatca ggccggcgac aggcgtgccg caaattgcgg gtacgtccgt
       61 gcgatgtcct cgcgtatgcg gccacgaatc gcggccagca cggcagcatc cggcttgcgc
      121 gtcacaggaa catccggggg gcagtcaaat tcgaacccgg tgttatcacg cacttcctcg
      181 agactgtggc cggggtgcac actgtcgagt cgaaaacggc gttgagcgcg gtcgaacgaa
      241 aacaacgcga gccccgtcaa cagtgcgtaa gggccgccgg gacggtacac accgggctcg
      301 cttacgccgg gcgcgctgat gaagtcgacc tttggcacca tgactcggcg cgtgtgctct
      361 tcgcgaaaca ggatgacgcg cggcaccagg aagtagagat aggccgatcc gaaagaaccc
      421 ggccagcgca cacggttatt gggataggga ccggcgccga ccagattgat gttggcctgc
      481 ccgtcgatct gtccaccgcc cagaaaaaaa gcatcgatcc ggccctgcgc ggcgagatcg
      541 aacaactcca cgccgccgct ggtaaaaaag ttgtccttga ttgatcccag cacgctgacg
      601 cgcatgctgc tcccggagtg cgatcgcgca agcagcgcac ccgcgccggg gatcggcgaa
      661 gatgcaccga cggcgacatg ggcgcagccg tgcaacaacc gcgcgatgac cgtgaccagc
      721 aattcggtgg gactgaattc ggtcatgccg cggcttgtac gccgctatcc cattggccga
      781 tgaattgctc cagcccttcc acggtgcgcg ctaaggtgat gtagcgagca attgccgcat
      841 cgtccatcgc ataaagatct gataaggcaa gcggccacgc gccgtgcttt gccaccgcaa
      901 tcgctgatac gtagatcgat ggcagcacgg ccccgccgcg ggccggatcg tcgagcagat
      961 ttccatccac aatttcttcc acagtgacga gggttgtttt cgcggcgtgg gccatgagca
     1021 gtacttcgcg ctctttaccg ataaatatat tgccctcgcg atcggcatag cgcgcatgga
     1081 acagcgcaac gtccggccgg atcgacggca gcaaaacgat tggatcaccg ggggaaaatg
     1141 gattatcgat gactttccag tcgctgcggt gacgcagcag atcggtgccg atcatgccgc
     1201 gcagcgggat gaacgggata tttttctcac ctgcctgaag cgcggcgtag attgccgggc
     1261 aggttgcatc caggatgcgc agcgacgcgc tgtgcaaagc ttgcgtgaag cagggggctg
     1321 tgccgaattc tcccaaggtg atcgctgaag tttccaccac gctcacggca cctgtgccga
     1381 tcaacagatc ggcctgcagc ccaccaaccg gcacgcacag taaatgtaag tcgtgcacct
     1441 tgcgccccag aagcgcacgc gtcgccgcca tggcgacgcc tgacgaatct ttcggcaccg
     1501 caagcagtgc accgtcgggg atcgcttcga ccagggtctc gagggaggtc agggaaggtg
     1561 cgttcatcaa tcggtggccg cgaccgggat ggatcaggca ttatggcgcg gaacaacgca
     1621 ggcgtctaac gcagttcagt gctcgcgccg ttttgccgat agcatgactt gcggatcgat
     1681 ctgcaccgat tgatggccat ccgcgaccca gacttgccca tcctgcatgg tgtattgcag
     1741 gtccatgttg cgggcggcaa gccgcccgag cgcctgcgtt ccggcatgcg gcaaattgat
     1801 taccgagagc ttgttcaaac gcgcgagctt gtcgctgacc tgttgccacc acaggtcagc
     1861 ggcacggccg ccgtaggtgt acacgagcac atgacccgcg cggccgctgg ctttgcgcat
     1921 gcgtttctca tctggcagac cgacgtcaat ccaggtttcg atgttgcccg tgagatcctt
     1981 gcgccacaag tcgggttcat cgtctgtgct caagcctgcg ccgaaactca gtgcctcgtc
     2041 tgcgttcaac gcgaaggcga gcaggcgcac catcatgcgc tcatcggttt cggacggatg
     2101 ccgcgcaatg gtcaacgcgt gatcatgata gtagccgcgt tccatgtccg cgatgtgcag
     2161 cgcggctttg aaaacggttg ccttgagggc catgtgatat ttgcggtcga tggtctagcg
     2221 gatggccgtg gcgtgcgggc aaaaaaatcc cgcgacgatc aagcgcggga ttttttttaa
     2281 gctgacgccc ggcgattgct gttacagcaa gggcaccaac aacaaggcaa cgatgttgat
     2341 gatcttgatc agcggattca cggccgggcc agcggtgtcc ttgtaggggt cgcccacggt
     2401 gtccccggtg accgctgcct tgtgtgcgtc ggaacctttg ccgccgtgat ggccgtcttc
     2461 gatatatttc ttggcattgt cccaggcacc gcctccggtc gtcatggaaa tggcgacaaa
     2521 caggccggta acgatggtac cgatcagcag gccgcccagt gcgcgaatgc ccgcgcctgg
     2581 tcccatgagc cagttcatgc cgaacgcgac tattaccggc accagcaccg gcaacaagga
     2641 aggaatcagc atctccttga tggcggcttt ggtgagcatg tctaccgcgc gcgaatagtc
     2701 gggcttggct ttgccttcca tgatgccttt gatgtcgcgg aactgccggc gtacttcgtt
     2761 gacgactgcg cccgcagcgc gtccgacggc ttccatggcc atggcaccga acagataggg
     2821 caccatgccg ccgatgaaca ggccgatgat gacggcaggg tcggacaatt cgaagccctt
     2881 gaccagcgcg cccgcgccga gcttatgcag cgattccagg ttgtgcgtgt agtccgcaaa
     2941 cagaaccagc gctgcgagac cggctgagcc gatcgcgtat cccttggtga cggctttggt
     3001 cgtgttgccg accgcgtcca gcggatccgt gatgtcgcga atcttcgccg gcagtcccgc
     3061 catttcggca atgccgccgg cattgtcagt gatcgggccg tacgcatcca gcgctacgat
     3121 catgccggtc atggacagca tggcggttgc cgcaatggcg attccgtaca ggccggcgaa
     3181 caggtaggac gcccagatag ccgcgcaaac cgcgagtacc ggcagcgcgg tggatttcat
     3241 cgatacgccg aggccggcaa taatgttagt ggcgtgaccc gtctgtgaag cggcagcgac
     3301 gtggcgcacc ggtccatatt cggtcgcggt gtagtattcc gtgatcacca ccatcagggc
     3361 agtcagcacc agaccgacca gggacgcgta aaacagattc atcgcgccga gcggactgtc
     3421 gcccatcatc ctgccggtga tgaagtaaaa cgcgatcgcg gcgagcacac cggatacgat
     3481 cacccccttg tagagcgcgt tcatgatctt gccgccctct ttcgccttga cgaagaacgt
     3541 gccgataatc gacgcgataa tggaagcgcc tcccagcacc agcggataca gtacggcgtg
     3601 ggggccgacc tggccgacca gcagaccgcc aagcaacatg gtggctacaa tcgtgaccgc
     3661 gtaagtttcg aacaaatcgg ctgccatgcc ggcgcagtcg ccgacattat cgccaacgtt
     3721 gtcagcgatc acggccggat tgcgcggatc atcttccggg atgccggcct cgaccttgcc
     3781 gaccagatcg gcacccacgt ccgcaccctt ggtgaaaatg ccaccgccca gtcgcgcgaa
     3841 aatcgagatc agcgaggaac caaacgcaag tccgaccagc gaatgcagcg ccttgtccgg
     3901 ctccactaac gcaagcagga tgccgtagta acccgccacg ccgagaaggc ccaagcctac
     3961 gaccagcatg ccagtaatgg cgcccccgcg gaatgccacc tgcagcgcgg cattgatgcc
     4021 gttgttggct gcttcggcgg tgcgcacatt cgcgcgcacc gagacgttca tgccgataaa
     4081 gccggtcagc ccggatagca ctgcgccgat caggaaaccg atggccgagt accagtcgag
     4141 cccgaatcca agcaacacga acaggaccac gcccaccatc gaaatggtcg cgtactgccg
     4201 gttcaggtac gcttgcgcac cttgttgaat ggcagcagcg atttcctgca tgcgcgcatt
     4261 gccggcgggt tggccgagta tccatttgat ggaaatcgcc ccgtaggcaa tcccgatgat
     4321 cgcgcagacc agcgcgaaga tcagtgctga agacatgaat cccccctcgt tttacagagc
     4381 cgaaaatggg ccggctcctg aaccctcgaa aaagaattgc cccggactgg tttgcatcct
     4441 gctggcgtgt ctggcagaag gtgcggtgag caaacgcgcg attctaaccg acttcgagca
     4501 acaccaaccg gtttgtgatc aaaccttgaa ctccgccagt ttgcgagcca cggcggtgtt
     4561 tttgagcgtc acgtacctag gcagtccatt gccgaacggc ggatagtcct ctccctggat
     4621 cagcggcgac aaatattggc ggcatttcgc ggtaatgccg tagccatctt cagtgatgaa
     4681 gtcacgcggc aattgttttt caacgttggc cacgtcactc aggttcgcga cgcccacggt
     4741 ccatttgtag ggcttgttcg atttacgcac gatggtcggc atcaccgcat tgcgaccctt
     4801 gaccgccagt tcgaccgcgg ccttgccaac ggcataagcc tgggccacat cggtcttcga
     4861 agcgatatgc cgcgccgagc gctgcagata gtccgccacc gcccaatggt acttgagggc
     4921 cagtccttcc ttcactacgg ccgccaccac cggtgccacg ccgccgagtt gcgcgtgccc
     4981 gaaggcatcg cgcagtccct ggtccgccag gaaaccgcca gacgagtcgc gcacaccttc
     5041 cgaaaccacc acggagcagt agccgtattg tttgaccagt gcttgaacgc gggcaagaaa
     5101 cgcgaccttt tcaaatggca cttccggaaa cagaatcatg atcggcaaat cacccggccg
     5161 gtcggctgcc aggccccctg cggccgcaat ccagccggcg tggcgcccca tgacttcgag
     5221 gatgaacact ttggtcgaag tcttcgccat ggacgcgaca tcgaagctcg cctcgcgcat
     5281 cgaaacggcg atgtacttgg cgaccgagcc gaatccgggg cagcaatcag tgaccggcag
     5341 atcgttgtcc accgtctttg ggacatgaat tgcctgcaag ggataaccga gggattcgga
     5401 caagccggaa accttgaggc aggtatctgc ggaatcgccg ccaccgttat agaaaaaata
     5461 gccgatgtcg tgcgccttga atacctcgat caggcgctcg tactgtgcgc ggttttcctc
     5521 cagccccttg agcttgtagc ggcaggaccc gaaagcgcct gcaggcgtgt ggcgcagctt
     5581 ggcaattgcc gtggcagatt ccttgctcgt atcgatcaga tcttccgtga gcgccccaat
     5641 gatgccattg cggccggcaa acaccttgcc gattttattt ttgtgcctgc gcgctgtttc
     5701 aatgacgccg gccgctgatg cattgattac cgctgtaact ccgccggact gggcataaaa
     5761 ggcattcatc ttcatttttc tctactccgc agcaggaaca tgaaccacag cgggattcct
     5821 acatttccgt ttcagcccgc tcgcagcaat taccggcgtc atttcacggc gctgaatatg
     5881 cggtcgcgta tcgcttccat ggagcctaca ccagcgacct tgacgtactt tggcgccccc
     5941 ggggtgccgc tgctggccca gttggagtag taagcgatta gcggcatggt ttgcgagcga
     6001 tagacctcaa gccgcttctt gatggtttct tccgtgtcgt ccgcgcgctg caccaaagcc
     6061 tcgccggtaa ggtcatcctt gccggccacc ttgggcgggt tgaaaatcac atgataggtg
     6121 cgccctgaag ccggatgaac acgccgacct gacagccgcc tgatgatttc ggcatcatcc
     6181 acatcgacct cgacaatcca gtcaagcatg acgccagcgt ttttcatggc atcggcttgc
     6241 ggaatggtgc gcgggaatcc atcgaacaaa aagccttttg cggcatccgg ctgtttgatg
     6301 cgttccttga ccaggccgat gataatgtca tcgggcacca gcgcgcctgc atccatgaat
     6361 ttttttgcct tgaggcccag cggtgtgcct gcgttgacag ccgcgcgcag catgtcgccg
     6421 gtcgaaattt gcgggatgcc gaattttccg gtgatgaagg cggcttgcgt gcctttgccg
     6481 gcgccgggcg cgccaagaag gatcagtcgc atttctctcc tcgtatgatt tacgttccca
     6541 accactggga gcgcggcctt tttaacagca gccgttgcaa gcgaattata cggtcgatca
     6601 gcggccggac tgaaacaccc ggcgcatatt ttccagatcc tccggcgtgt cgacaccggg
     6661 atcgggcgcc tggtccgtga ccacaacgct gatacggtag ccatgccaga gcgcgcgcaa
     6721 ctgttcgagg gattcgaact gctcgatagc agcgggcgcc agagtcgaat agatcttcag
     6781 aaaactgcag cggtacgcgt acagaccgag gtgccgatag gctggcagtc cgcggggaag
     6841 gtttgatgtg gaggttttga atgcatcacg cgcataggga atgggcgcgc gggaaaaata
     6901 gatcgcgtag ccttgctggt cgaggacaac cttgaccgcg ttggtatcga acatttcgct
     6961 tgccgcatgg acccgatggc aggcggtggc gatggctgca tcactgtgcg cggcaagctg
     7021 ttcggctacg gagcgtagca gttgcgacgg catgcgcggt tcatcgccct gggcattgac
     7081 cactatgagc tcgtccggcc agccgcgaac ggccgcaatt tccgcgatgc ggtccgtgcc
     7141 ggaagggtga tccggtcgcg tcatcagcgc ctggtgcccg tgttttgcga ccgcttgctg
     7201 aattctctgg tcgtccgtcg cgacgatgac ttcgtgtgcg ccggattcac gcgcccgatc
     7261 tgccacgtgt accaccatgg gtttgccggc tatgtctgca agtggcttgc caggcaggcg
     7321 cgcggaggcg tagcgggcag gaatcacgac aacgaatcgc atggtgtcag taacgggccg
     7381 aacggtaagc ggtgaagccg gatcctcctg gaagcggatc acccgttagc gttcaccgta
     7441 cttcctcctc cggcggcaac ttgcgcgctt cgtcttccag cattacagga atgccgtcga
     7501 cgatcggata cccgagccgg caggccttgc acaccaattc tttttcggcc tttttgtaca
     7561 gcagcggacc cttgcacaag gggcagacca gaatatcgag cagtttagga tccatgccgg
     7621 tttttcagtt tctccacaat cagcctgcca aggtccgtgt cgaccactgc gtccactggc
     7681 aatacccaat ggttttcacg cgcgaagggc aggcatttta tcgcatcctt ctcggtcatg
     7741 atgatggcat cggcattggc aaactccaga tcgcttgcag aatacgcatg atgatctgga
     7801 aacgggtgcg ccacgaaatt gagcgtcata cgctgcaact ggtcaaaaaa gcgtcccggg
     7861 ttgccgattc cggcgattgc gtgaacgtgt ttgccctgga aggctgccgc atcctggtga
     7921 aaggccggat taagcaaatt gcggaacatc gtgccttcca aagccatccc gaagtttggc
     7981 ttgccggcaa ccggtgccaa tgcattgccg gtgatcacca ccgcatcgac gcggttcagt
     8041 cggcgggtcc cttcccgcaa cggaccggcc ggcagtggaa gaccgttgcc ggtgccgcgg
     8101 ctgccgtcga tgactgcaat ttcaaagtct cgcgccattg cataatgttg cagaccatcg
     8161 tcactgacga tgacgttgca ttcagggtga gccgcgatga gtgccctcgc agccgcagcg
     8221 cggttacgcc cgatccagac cgggcggcgg cttcgcatcg ccagcaatac cggctcatcc
     8281 cccacgattt caggcggcga actcggcatc acggccatga tttggccgcc gccgccatag
     8341 ccgcggctca cgatgcccgg tgcgtagcct tgctccgtca aaaaatcgca cagccacagg
     8401 accagcggcg tttttccggt acccccgaca ctgatgttgc cgaccacgat caccggcacg
     8461 gcaagccgat acgaatgcaa taagcctgag ccgtagagta aacgccgaat tgcagccacg
     8521 catgcgaaca gcgcggcgaa tgggagcagc aggatggaca gccaggaaag gcgataccag
     8581 tgacggtcga ggaatgccag ggtgggtccc tctttagcga gtcttgcaac gattcacttt
     8641 acgccacgcg tcgcgcggat tggaatcgcg ggcttcagcg acgctgttgc tgggtggtaa
     8701 acgtgatttt gctgaaaccc gcgacccgtg cggcttccat gacgttgata actgactggt
     8761 gcgtggcatt ggcatcggcg ttgatgatga tgaccgggtc cgtgttgctg cctgcggcgc
     8821 gcttgagttc ggttgccagg gcttccgggg tcgtaaatcc cacggcggca cggttgatca
     8881 tatattgacc gttggaggac acgccgacgt tgatctggtt cggtcgatct gcaggcttgc
     8941 tggcatccgc cgtcggcaga ttgatctgca tttcagaata gcgggaatac gtggtcgtga
     9001 ccgccagaaa tatcacgatg acgatgagga tgtcgatgaa tggaaccagg ttgatttccg
     9061 gctcctccct gaacgaacca cggcggaaat tcatgaccgc gcagttcagg cctgacgttg
     9121 cccgtgaatg atctcgacca gcttgactgc ctgaacttcc atttcgacga ccagtgaatc
     9181 cgcgcgggcg cggaaatgac gatagaacac gaggctcggc acggctacga tgatgccgaa
     9241 ggcggcgttg tataaagcaa ttgaaatacc atgcgccaac tggcccggat tcgccatccc
     9301 cgtggggccc tgcgagccaa agatttcgat catgccgatg atcgtgccaa gcaaaccaag
     9361 caagggtgcc atggtggcaa tggtaccaag cgtcgtcagg aagcggccca gctcgttggc
     9421 aacggctctt cctgcttctt cgatcgactc tttcatgatc tcgcgcgaac ttgccgcctc
     9481 ttttagagcg gcggcaaaaa ttctgcccaa catcgcgcct tgtgccagcc ggtcaagcat
     9541 gtctgctgaa acacctttct ggcgatattc ctggacaacc ctgggtagca gatcgcgtgg
     9601 cgcgacaaaa tcttttctta aggtgtagaa gcgctcacca atgatgccga gcgtgatgat
     9661 agagcacaga attaatggcc agatcggcca accggccgct tctatgatgt gtagcaagtc
     9721 aactccgcag atttcaaacc cgcgccactt taaactgcat cgatatgcgc agcaagcgaa
     9781 ccggtatgca ggcgagatgc tcttgacaag cgaaccggtt gacgtccatc acgtaaaatc
     9841 tgtccacaat ctttgtggat aactatgtgc atagtttgta caaagacagg gtaagtcgat
     9901 tctgcgaaag ctttttttgt aggatgatta aaaaagcggc gtgcactatt ccatgcaaat
     9961 acagtaactt attatgacac cccctgtagt catgcgggtt tccgggcgat cgtatgtcac
    10021 tttggcgtca actgtggacg aaaggaacga cacgcgcgtt cgttgtcaag acaaaaatcc
    10081 agtctgcgtt tccgcgttgc acaccgggac tcagcggaat ggcgtgctgt ctgtgatctg
    10141 gaatgctgcg catgcatatc gatgatgcaa acgtagcggt catcagcgtc gctgaactga
    10201 atcgtcgagc gcgcgaattc atcgaaacgg gattcccgct gctctggatc gcaggagaga
    10261 tctgcaactt cactcgtgcc gcgtccggcc actgttattt ttcgttgaaa gatgccaccg
    10321 cgcaggtgcg ctgcgtattt ttccgcaaca aggcggctct cgttggatgg aagctggaaa
    10381 acggcatgca agtcgaagtg cgcgcgttgc ctacgttgta tgaagcgcgc ggcgattttc
    10441 agttaaccat cgaaggcatg cggcgctccg gactcggcgc tttatttgag gcattcgagc
    10501 gcctgaagct aaagctcgaa caagaagggc tgttcgatgc gttacgccgc aagccgattc
    10561 cgcaatttcc tgcggcggtc ggcgtggtta cctcgctgac gggcgcggtt ttgcatgacg
    10621 ttctgacgac tctggggcgg cgcatgccat cgattcgcgt ggtggtttat cccgcgcccg
    10681 tgcaaggcga gggcgcggga acgcggattg cccgggcaat ccgcatggcg tcgatgcgaa
    10741 gggaagtcga cgtgctgatt atttgccgcg gtggcggcag catggaggat ctatgggcgt
    10801 ttaacgagga aatcgttgct cgtgccatcg ttgagtgcgg catcccggtc gtctccggca
    10861 ttgggcatga aaccgatttc accatcactg attttgttgc cgatgcgcgc gctccgacac
    10921 ccactgctgc tgccgagctg gtgagtccga accggttcga actgctggcc cgagtcggtg
    10981 tcctgggcaa acggctgtcg cgcgatattc accgtgcgct gaacacccgc atgcagcaaa
    11041 ttgattactt gacgaagcgc ctgatgcacc ccggtgagcg tatgcaggca gaactgcgtc
    11101 atttggcaca cttggccaac cgcatgcacg cggcatccag gcggacgatc gatgcagcag
    11161 cctggaacct gtctgatctg tcgcggcatt tacggggcac gcttccggat gtggatgcat
    11221 tgattgaaaa gcaacaagca ttgcagcagc gcctgcgttt ctcccaggcg cgcgtgcttg
    11281 agcagcacgc tgccagcctg ggcaaagcca aggcaaacct ggagcacctc aatcctcatg
    11341 cggtgcttga ccgcggctac agcattgtgc agaaaaatga cggacgaatt gtgggcgccg
    11401 cccaacagat tgctactggt gagttgctgc gtttgacgtt tgccagcgg
//
LOCUS       DATMZQ010000542         2643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1707556, whole genome shotgun sequence.
ACCESSION   DATMZQ010000542 DATMZQ010000000
VERSION     DATMZQ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707556"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VN496_17395"
     CDS             complement(<1..317)
                     /locus_tag="VN496_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18786.1"
                     /translation="MLFQSQFYVLVFLPVAAALYYSVANWTIGRQWVLIGLSLIFYGW
                     WDVRFVALPVAQIAATWLLAGVHVRLQSRAPLIIGVALNLLSLGTFKYLDFLLASIEA
                     ASG"
     gene            745..1932
                     /locus_tag="VN496_17400"
     CDS             745..1932
                     /locus_tag="VN496_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXF18787.1"
                     /translation="MPIVSLSAGFVRRVVCVPARKKVDYFDVSQRGFLLEVRQSGGKT
                     YYQRYTDERGRERQYKIGPAEVLTLDQARRKGKQVVAEALLGADPQQKRRDMRAVLQL
                     GRFVHERYLPYVRTYKNSWQTDETVLRIHILPKLGALALDEIKTDAISELINSMRADG
                     YASGTINRVIVILRYIYNLARKWKTPGGADNPAAGLSAGPDVQRNRFLSEEEAEALVQ
                     AIRADQNQTAARAIMLLLLTGARRNEITFAKWDYVDWKNKTLLVPKSKSGRARAIALN
                     RSAIALLTAAPRIDGNPYIFPSEVNGRPPASLYFPWDRIRKRAVLPDVRLHDLRHSFA
                     SFLVNRGVSLYVVQGLLGHLHARTTQRYAHLTQETLIEAADLIQDVVTFPGVVSPLGR
                     PSN"
     gene            2220..>2643
                     /locus_tag="VN496_17405"
     CDS             2220..>2643
                     /locus_tag="VN496_17405"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXF18788.1"
                     /translation="MKICSLYGVGFYYIPGTDMCIKIGGWVRAEYGWGQNGNFAWGWA
                     NANVNNRTTNNSDFRARGYITADARNQTEYGTVRGYIAVGLSENEHGGDVAASNNFSA
                     NRAFIQWAGFTFGRAQSFFDFYSNPATSYWGAFPGSDTG"
BASE COUNT          587 a          768 c          744 g          544 t
ORIGIN      
        1 attccgctcg ctgcctcgat cgaggccagc aggaaatcca gatacttgaa tgtccccagc
       61 gaaagcaggt tgagcgccac gccaatgatg agtggtgcac ggctctgcaa tcgcacgtga
      121 acacctgcca gcagccaggt cgccgcaatt tgtgcaaccg gcaatgcgac gaaacggacg
      181 tcccaccagc cataaaatat caacgacagt ccaatgagga cccattgcct gccgatcgtc
      241 cagttggcca cgctgtagta gagcgccgcg gcgaccggca gaaagacgag cacgtaaaac
      301 tgcgactgaa aaagcattga tccgtctgcc cacctcggtg gcttgctcca ctctgtcgaa
      361 ccacgtcgag tgagttcaaa ggaccttcgg ttcgaatcct gctgcgtctg acaccagcgc
      421 gacacatcct attttgtttg tgcgtgcggg cagcccagaa tgcacctgac aatgaccaaa
      481 agacctccgt ccagaggatg gtgcttgtcc acacaccgga atagcatcca aaaccatgta
      541 tgaaggtaac gccaagaaga taagcaataa caggaacaaa caggtaggta acatgctaat
      601 ctcaaaagat cataaattaa gatttttctt gccagtagtt gtgctttacg caaacatcga
      661 agggctctga agttgtaacc cgccacagac gtacaactcg ccgctttcgt ggggccgaaa
      721 gcgaggcatt gggcagtcgt gtaaatgcca attgtttctt tgtccgccgg ctttgtgcga
      781 cgggtggtct gcgttccagc ccggaagaag gtcgactatt tcgacgtgtc gcagcgtggg
      841 tttctgctgg aagtccggca gtccggcggc aaaacctact atcaacgcta cacggacgag
      901 cgtggccgcg agcggcagta caagatcgga ccagccgaag tcctcaccct ggatcaggca
      961 cgccgcaagg gaaaacaggt cgttgcagaa gcgctgctcg gcgcggatcc gcaacaaaag
     1021 cgccgcgaca tgcgggcggt gctccagctg ggccggttcg tgcacgagcg atatctgccg
     1081 tatgtgcgca cgtacaagaa cagctggcag accgatgaga cggtgttgcg catccacatt
     1141 cttccgaaac tcggcgcgct cgcgctcgac gagatcaaga ccgatgcgat ttccgaattg
     1201 atcaattcga tgcgcgcgga tggctatgcc tccggcacga tcaaccgcgt catcgtgatc
     1261 ctgcggtata tctataacct ggcgcgcaaa tggaagacgc ccggtggcgc cgataatccg
     1321 gcggccggat tgagcgccgg ccccgatgtg caacgcaatc gttttctcag tgaagaggaa
     1381 gccgaggccc tcgtgcaggc aattcgtgcc gaccagaacc agaccgccgc acgcgcaatc
     1441 atgctgcttt tgctgaccgg cgcgcggcgc aatgagatta cgtttgccaa gtgggactat
     1501 gtcgactgga aaaacaagac tctgctggtg ccgaaatcga agtcagggcg ggcgcgcgcc
     1561 atcgcgctca acaggtcggc aattgctctt ttgacggcgg ctccgcgcat tgacggcaat
     1621 ccatatattt tcccatccga agtcaacggt cggccgccgg catcgttgta ttttccctgg
     1681 gatcgtattc gcaagcgcgc ggttctcccg gatgtccgcc tgcacgacct gcgccactct
     1741 tttgcgagct tccttgtcaa tcgaggcgtg tcgctctacg tcgtccaagg cctacttggg
     1801 catctgcatg cgcggacgac gcagcggtac gcacacctca cacaggaaac gttgatcgag
     1861 gcggcggatc tgatccagga cgttgtgacg tttccgggcg tcgtttcccc gctgggacgg
     1921 ccctcgaact gaataacagg aaatttgacc gctatgcttg tctggtcatc ctgaccagcg
     1981 tcgttggcat tcaacaaacg actcaggatg cgtcctgcga tatacctatt tggttggctg
     2041 catcggccat cgttgtgacg acgcagaact tccatcaatc aagtacttaa tttttcgcct
     2101 gacccacgcc acctctagtg tggactgttg caggctgaac gaattgcgat tgtcttggcc
     2161 gcgtcacagc ctctatcatt gaatattgcg ggatcccgcg ttttcaattc gtccagtacg
     2221 tgaagatttg cagcctgtac ggtgtgggat tctattacat cccgggcacc gacatgtgca
     2281 tcaagattgg cggctgggtc cgtgccgaat acggctgggg ccagaacggc aattttgcct
     2341 ggggttgggc gaacgcaaac gtcaacaacc ggacgaccaa caacagcgac ttccgcgccc
     2401 gtggctacat cacggctgat gcccgcaacc agaccgaata cggtacggtg cgcggttaca
     2461 tcgcggtcgg cctcagcgag aacgagcacg gcggcgacgt cgcggcatcg aacaacttca
     2521 gtgcgaaccg cgccttcatt cagtgggcgg gtttcacgtt cggccgtgcg cagtcgttct
     2581 tcgacttcta cagcaacccg gcgacttcat actggggtgc cttcccgggt tccgacacgg
     2641 gtg
//
LOCUS       DATMZQ010000543         5791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1709316, whole genome shotgun sequence.
ACCESSION   DATMZQ010000543 DATMZQ010000000
VERSION     DATMZQ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709316"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="tolA"
                     /locus_tag="VN496_17410"
     CDS             complement(<1..872)
                     /gene="tolA"
                     /locus_tag="VN496_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HXF18789.1"
                     /translation="MQSLREPGRVASGALAVLVHLMFFGFLVFGLSWQKKIANPIVVD
                     LWQELPEPAKKSAPLPEPPPPPAPKVIPKPPPPAPVPEVKKVVPPTPPKVEAPKPSAA
                     DIELKEKLRKQKEEEAERKEEQRKAEAERKEEQRKLEAERKEELKRAELEKRKLEEER
                     KEELKKAELEKRKAEDEQRKIEAERKEAEKKAELEKRKLEEEQRKVAEEQRRAEQEKL
                     RQDEEARRAEAAKRAEDQRRAEAEQKRIEQEKLRQQQEARRAEAAERAAHQKMIDDWK
                     AKIQARIKSRVVVPP"
     gene            complement(876..1301)
                     /gene="tolR"
                     /locus_tag="VN496_17415"
     CDS             complement(876..1301)
                     /gene="tolR"
                     /locus_tag="VN496_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HXF18790.1"
                     /translation="MAANRRRHPRLMNQINVVPYIDVMLVLLVIFMVTAPLMNPGVID
                     LPSIGKSSAPPAMPMEISIRAGGALSLIDRDISNAERPVNRDELIKMIKSKQAKKPDQ
                     PVVISADKDVKYEAVIQVMDMLQQNQVKRVGLLAKPRSG"
     gene            complement(1309..2037)
                     /gene="tolQ"
                     /locus_tag="VN496_17420"
     CDS             complement(1309..2037)
                     /gene="tolQ"
                     /locus_tag="VN496_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HXF18791.1"
                     /translation="MNVSQDLSLISLIGNASVLVQLVLALLFVVSVISWWFIFRKMFV
                     VRDAIRRTNQFERSFWSGADLGTLYQAAASARHDAGSMERIFESGFREFVKLRKQSGA
                     SLVMIMEGTRRAMRATYQRELDRLEAHLSFLATVGSVSPYVGLFGTVWGIMNAFRGLA
                     NVGQATLAHVAPGIAEALVATAMGLFAAIPAVIAFNHFVRHIERLSTRFDSFQEEFSN
                     ILQRQAGAQTVGAHTGTHPVFNPG"
     gene            complement(2055..2603)
                     /gene="ybgC"
                     /locus_tag="VN496_17425"
     CDS             complement(2055..2603)
                     /gene="ybgC"
                     /locus_tag="VN496_17425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HXF18792.1"
                     /translation="MHRRTGIWAWRCPLLRALLNSGGRLNILFEIPVRVYYQDTDAGG
                     VVFHAAYLDFLERARMDWLRARGFDAKRLLGRYHLFFIVRKLELAYVKPALLDDLLVV
                     SAALQKLGHAQLTFEQHVRRDGETLLRGTVNVACVAAGSLKPSPFPEEIRASLAEEAE
                     AATATTSQLTRTGQPRRHLNRV"
     gene            complement(2527..3567)
                     /gene="ruvB"
                     /locus_tag="VN496_17430"
     CDS             complement(2527..3567)
                     /gene="ruvB"
                     /locus_tag="VN496_17430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXF18793.1"
                     /translation="MIETDRLITPDPQSTHEEALERALRPKQFEEYIGQEKIRGQLQI
                     FIQAARKRGESLDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPALEDFQLDIMIGEGPAARSVKLELPPFTLIG
                     ATTRAGMLTNPLRDRFGIVSRLEFYSPAELTRIVQRSAGLLNVETTPEGAFEIASRSR
                     GTPRISNRLLRRVRDFAEVKAEGVVDRDIADAALKMLDVDRIGLDVMDRKLLLAVVEK
                     FGGGPVGIDNLAAAIGEERDTIEDVLEPFLIQQGYLQRTPRGRMATASTYRHLGMAVP
                     SASGTAELWGEA"
     gene            complement(3602..4384)
                     /locus_tag="VN496_17435"
     CDS             complement(3602..4384)
                     /locus_tag="VN496_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXF18794.1"
                     /translation="MTAAARAVLLIMAVLTVPAGVFAATETSANKQRQIEQLIELHDL
                     TTSVSIGNFYLKQEGLIAIRGLLTRLGNQDKLGPGWNSHDPHWQRAEDLLLNEVVDKV
                     AQDFTSLDWLRPQWQELDSKEFSAEEVAALLNHFRSDVGRKQAMIVDHTVSTHVMATL
                     SFSGKLKDIPGIQSERNRMQTAWNKEDDDMRFSIQDATNADGQRFAYSALGKKYFVTA
                     ILKLTGIISHRLDELAQALPQQIDARAEDVRPLLQEFKSGRG"
     gene            complement(4535..5113)
                     /gene="ruvA"
                     /locus_tag="VN496_17440"
     CDS             complement(4535..5113)
                     /gene="ruvA"
                     /locus_tag="VN496_17440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXF18795.1"
                     /translation="MIGRLAGQLLEKRPPTIVIDVQGVGYEVDVPMSTFFLLPATGAA
                     VTLHTHLVVREDAHLLFGFATDEERQVFRKLLKISGIGARTALSVLSGMSVSELYQAV
                     SAHDGARLTRIPGIGKKTAERLLLELRDKLNAGVGLTTSSGEAGIRSDALNALLALGY
                     SDKEATHALAKITPETPVPEAIRSALKLLSKV"
     gene            complement(5110..5637)
                     /gene="ruvC"
                     /locus_tag="VN496_17445"
     CDS             complement(5110..5637)
                     /gene="ruvC"
                     /locus_tag="VN496_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXF18796.1"
                     /translation="MTVRILGIDPGLRVTGFGVLEIAGQRLTYVTSGRIRTADTGELP
                     ERIRTILEGLSRVIAELKPQQVAVEKVFVNINPQSTLLLGQARGAAICAAVIGALPVS
                     EYTALQVKQSVVGNGHARKEQVQEMVRRLLKLPGDPSADAADALACAICHAHGGLGLG
                     KLQTRGFRMKRGRLV"
BASE COUNT         1087 a         1763 c         1661 g         1280 t
ORIGIN      
        1 ggcggcacaa caacgcggct tttgatcctg gcctgaattt tcgctttcca gtcatctatc
       61 attttctgat gcgcggcgcg ctcggcagcc tcggcgcggc gcgcttcttg ctgttgccgc
      121 agcttttcct gttctatacg cttctgttcc gcctccgcgc gccgctgatc ctcggcgcgc
      181 ttggccgcct cggcccggcg cgcttcttcg tcctgccgca gcttttcctg ttctgcacgc
      241 cgttgctctt ccgcaacctt gcgctgctct tcttcgagtt tgcgcttttc cagctccgcc
      301 tttttctccg cttccttgcg ctcagcttca attttgcgct gctcatcctc cgctttgcgc
      361 ttttccagct ccgctttttt cagttcttcc ttgcgctctt cttcgagttt gcgcttttcc
      421 agctctgctc ttttcagctc ttccttgcgc tcagcttcaa gtttgcgctg ttcttccttg
      481 cgctcggcct cggctttacg ctgctcttcc ttgcgctcgg cttcttcttc cttctgcttg
      541 cgcagcttct ccttgagctc gatatcggca gccgagggtt taggggcttc aaccttgggc
      601 ggcgttggcg gcactacttt tttgacttcc ggcaccggcg cgggcggtgg cggcttcggg
      661 attaccttgg gtgccggcgg aggcggcggc tccggcagcg gcgctgactt cttggccggc
      721 tcgggcaatt cttgccataa atccaccacg attgggttcg caattttttt ctgccagctc
      781 aatccgaata ccagaaagcc aaaaaacatc aggtgcacca gcactgcaag tgcgccagaa
      841 gcaacgcggc cgggttccct gagcgattgc accctttacc ccgaacgtgg cttcgccagt
      901 agtccaacgc gcttcacctg attctgctgc agcatgtcca tcacctgaat cacggcttcg
      961 tatttcacgt ctttatcggc cgaaatgact actggctgat ccggcttctt cgcttgcttg
     1021 ctcttgatca ttttgatcaa ctcgtcgcga ttgaccgggc gctcggcatt cgatatgtca
     1081 cggtcaatca acgacagtgc gccgcccgcg cgaatggaga tttccatggg cattgccggt
     1141 ggcgcggacg atttgccgat tgaaggcaga tcgatcacgc ctggattcat cagcggcgcc
     1201 gtgaccatga aaatcaccag taaaaccagc atcacgtcga tataaggcac gacgttgatc
     1261 tggttcatca gacgcgggtg acgccggcgg ttcgcagcca tgagttaatc agcccgggtt
     1321 gaagacaggg tgagtgccag tatgtgctcc aaccgtttgc gcgcccgcct gacgctgcag
     1381 gatattggag aactcttcct ggaagctgtc gaagcgcgtg gacaggcgct ctatatggcg
     1441 cacgaaatgg ttgaaggcaa ttactgccgg gatcgctgcg aacaggccca tggcagtggc
     1501 taccagcgcc tcagcgatgc cgggtgcgac gtgggccaag gtcgcctgtc ccacgttggc
     1561 gagtccgcga aaggcattca tgatccccca caccgttccg aacaggccaa cgtaaggact
     1621 gacggaccca accgtagcca ggaatgagag gtgcgcttcc aagcggtcga gttcacgctg
     1681 gtaggtcgcg cgcatcgcgc ggcgggtgcc ttccatgatc atgactaggc tggcgccgct
     1741 ttgtttgcgc agcttgacga attcgcgaaa cccggattcg aaaatgcgtt ccatgctgcc
     1801 cgcatcgtgg cgcgcgcttg cggccgcctg ataaagcgta ccgagatcgg cgccgctcca
     1861 gaaacttcgt tcaaactgat tcgtccgccg aatggcatcg cgcacgacaa acatcttgcg
     1921 aaagatgaac caccaggaaa tcacggacac cacaaaaaga agggccaata ccaactgcac
     1981 cagcacgctg gcattgccga tgaggctgat caaggacagg tcttgggaaa cattcatggt
     2041 tgtgtatgac tgtcttaaac gcggttaagg tgccgtcggg gttgaccggt ccgggtaagt
     2101 tgactggtgg tcgctgttgc cgcttctgcc tcttcagcta acgaggcacg aatttcctct
     2161 ggaaacgggg atggcttgag gctgcctgct gcaacacagg caacatttac cgtgccacgc
     2221 agcaacgttt caccgtcgcg cctgacgtgc tgctcaaacg tcagctgtgc gtggcccaac
     2281 ttttgaagcg cggcgctgac aacaagtaaa tcatcgagta gtgctggttt tacgtaggcg
     2341 agctcgagtt tgcgaacgat aaagaataga tgatagcggc ccagcaagcg cttcgcgtca
     2401 aagccgcgcg cccgcaacca gtccattcgc gcacgctcca gaaagtcgag ataggccgca
     2461 tggaacacta cgccgccggc atcggtgtcc tggtaataca cgcgtaccgg tatctcgaac
     2521 aggatgttaa gcctcccccc agagttcagc agtgcccgaa gcagagggca ccgccatgcc
     2581 caaatgccgg tacgtcgatg cagtagccat gcgaccgcgc ggcgtacgtt gcaagtaacc
     2641 ttgctgtatc aaaaaaggct cgagcacatc ttcgatggtg tctcgttcct caccaatggc
     2701 cgcggccaga ttatcgatgc ctaccgggcc accgccaaac ttctcgacga cagccagcag
     2761 cagctttcga tccatgacat ccaggccgat acggtccaca tccagcatct tgagtgccgc
     2821 atcggcgatg tcccggtcca ccacgccttc ggcctttact tcggcgaaat cgcgcacgcg
     2881 cctgagcagg cggttggaaa tgcgcggggt accacgcgag cgcgacgcaa tttcgaatgc
     2941 tccttcgggc gtggtttcca cgttcagcag accggccgag cgctgaacga tacgcgtgag
     3001 ttccgccggc gaataaaatt caagacggga aacgatcccg aatcggtcac gcaacgggtt
     3061 ggtcagcatg ccggcccggg tcgtggcacc gatcagggta aatggtggca gttcaagttt
     3121 gaccgaccgg gcggcagggc cttctccgat catgatatcg agctgaaaat cttccagcgc
     3181 aggataaaga atctcctcga cgaccggcga caagcggtga atttcatcga tgaacagcac
     3241 gtcattgggt tcaagattgg tcagcagggc cgcgagatcc ccgggacgtt ccagcaccgg
     3301 tcccgaagtc tggcgcagat ttacgcccat ctcccgcgca atgatgtggg caagcgtagt
     3361 tttccccagt cccggcggcc cgaacagcag tacgtgatcg agcgactcgc cgcgcttccg
     3421 ggcagcctgg ataaatatct ggagctggcc gcggatcttt tcctggccta tatattcctc
     3481 gaattgcttg ggacgcaagg ccctctcgag cgcttcttcg tgcgtcgact gtgggtcagg
     3541 cgtaatcagc cggtcagtct cgatcatcct ttgtagctgc tgtgaaatcg cgcttggcgc
     3601 atcatccacg gccgctcttg aactcctgca acagcggccg gacgtcttcg gcgcgcgcat
     3661 cgatctgttg gggtagcgcc tgtgcgagct cgtcgaggcg gtggctgatg ataccggtta
     3721 acttcaggat cgctgtcaca aaatattttt ttcccaacgc cgaataggcg aagcgctggc
     3781 cgtcggcgtt ggtcgcatcc tggatggaaa atcgcatgtc gtcatcttcc ttgttccagg
     3841 cggtctgcat gcggtttctt tcggactgga ttccggggat gtccttgagc ttgccggaga
     3901 acgacagcgt cgccatcacg tgagttgaca cggtatgatc aacgatcatc gcctgcttcc
     3961 ggccgacatc ggaacgaaag tggttaagca gcgcggctac ttcttcggcg ctaaattcct
     4021 tggagtcgag ttcctgccat tgcggccgca gccaatccag actggtgaag tcttgcgcca
     4081 ccttgtctac aacttcgttc agcagcaaat cttcggcgcg ctgccagtgc ggatcatggg
     4141 aattccagcc aggtcccagc ttgtcctggt tgccgagccg cgtcagcagc ccgcgtatgg
     4201 caatcagacc ttcctgcttg aggtagaagt taccgattga taccgatgtc gtgagatcgt
     4261 gcagctcgat gagttgctca atttgccgct gcttgttggc gctggtttcg gtggcggcaa
     4321 aaactccggc tggcaccgtc agcacggcca tgatcaatag aacagcgcgt gctgcggcag
     4381 tcatcggtaa acatcctcgt tcatcaagca agacccggtc actccataaa attgccccgg
     4441 attatagcct agcgcccggt gtaaacgtgt ctcgccgatg catgcggtgt gtcgtgcgcg
     4501 ggcgacattg cgtgtcatgc cagcgcgcgt cgaatcagac cttcgacagc aatttaagcg
     4561 cactgcggat tgcttccgga acgggcgttt cgggtgtgat tttggcgagc gcgtgcgtgg
     4621 cttccttgtc gctatagccc agtgccagca aggcgttgag cgcatcgctc ctgattccag
     4681 cttcgccgct cgaagtcgta aggcccacgc ccgcgttgag tttgtcgcgc agttcgagca
     4741 gcaggcgctc cgctgtcttc ttgccaatgc cgggaatccg ggtgagccgg gcgccgtcgt
     4801 gcgccgatac cgcctggtac aactccgaca cggacatccc ggacaatacc gatagcgcgg
     4861 tgcgtgcgcc gatgccgctg atcttgagca atttacggaa tacctgccgt tcctcgtcag
     4921 tcgcaaagcc gaacagcagg tgcgcatcct cgcgcaccac caggtgtgta tgcaaggtga
     4981 cggcggcgcc agtggcgggc agcaggaaaa aagtactcat cgggacgtcg acttcgtagc
     5041 ccacgccttg cacgtcgatg acaatcgtcg gtggacgctt ttccagcaac tgacccgcaa
     5101 gccgtccgat cacaccagcc tcccgcgttt cattcggaag ccgcgcgttt gcagcttgcc
     5161 gaggcccaga ccgccatgcg cgtgacaaat cgcgcacgcc agcgcatcgg cggcatcggc
     5221 acttggatca ccgggcagct tcagcaggcg ccgcaccatc tcctggactt gctccttgcg
     5281 tgcatgacca ttgccgacta ccgactgttt gacctgcagc gcggtatatt ccgataccgg
     5341 caaggcgccg attaccgctg cgcagatggc ggcgccgcgc gcctggccga gcagcagcgt
     5401 agactgggga ttgatattga cgaatacttt ttccacggcg acctgctgcg gtttgagttc
     5461 tgcgatcact cgcgacaggc cttccaggat agtgcggatg cgctccggca gttcaccagt
     5521 gtccgcggtc cggatgcgcc cgctggtaac ataggtcagc cgctggccgg cgatttcgag
     5581 tacgccgaat cctgtgacgc gcagccccgg atcaatacca agaatgcgta ccgtcacggt
     5641 gtgagcccga acggcgcgcg gggcgcaaac caggcgggcg ccgggaacga aaggcgggct
     5701 gagcaggcgc tggtcaaatc caccgcgcgc gtttcatgcc ggcgtcgcct cgtccatgac
     5761 tgcgctgctg tagacttcct gcacgtcgtc g
//
LOCUS       DATMZQ010000544         4868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11139
            k141_1722996, whole genome shotgun sequence.
ACCESSION   DATMZQ010000544 DATMZQ010000000
VERSION     DATMZQ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945715
            Sequence Read Archive: SRR6185295
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:32:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,472
            Genes (coding)                    :: 3,451
            CDSs (with protein)               :: 3,451
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 5 of 21
            Pseudo Genes (incomplete)         :: 15 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722996"
                     /isolate="SMAG_U11139"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN496_17450"
     CDS             complement(<1..407)
                     /locus_tag="VN496_17450"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXF18797.1"
                     /translation="MVKSTELLRDITSDQWLEALTSRLAPVERDLVISADAWARQHYP
                     GLLHPTGQAWYDHARGAAGTLGTMRVGGEAIAAMMLLGAPLSTKVERESLQRVFGATV
                     IALVDGVASMAQIQLLRRQAATESKTSGRALQL"
     gene            complement(424..2760)
                     /locus_tag="VN496_17455"
     CDS             complement(424..2760)
                     /locus_tag="VN496_17455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HXF18798.1"
                     /translation="MTSIYLFAFIAGAWLLQQQVVLPHWPAAAVLVPLTALCMFLFRA
                     SRPANRVSLHAAAIVTFAAAGFFWAAAIAHWKLSDELPEIWQGRDIEISGVIAEMVQP
                     NERGVRFVLDVDRTYTLNAKIASRISLMWYQTDNASLPTLHAGQYWRLVVRLHRPHGN
                     ANPDGFDFEAWMLERGIRAVGYVRADPAPHLIKPLVWRAGYLLERCREAARAHLQAAL
                     GQRPYAGVVVALAIGDQQAIPADQWQVFTRTGVNHLISISGLHITMVASMAVLLILRL
                     WRLSETLMLKLPARRAAAICGLAVAIGYAVLSGFAVPAQRTVYMLAVVAVALWNNWTT
                     RPISVLAAAAALVVVLDPMAVMAPGFWLSFGAVAVIMLTAGSRIGRQGWFRSWIQIQG
                     AVTLALIPLLLALFQQISLVSPLANAFAIPVVSLIVVPLALLAAVIPVDGLAYAAHQL
                     MALCMIVLNMLSNWPAAVWQQHAPPAWTIPIAIAGAIWMLLPKGFPARWLGASLMLPL
                     FLSAPDSVPLRELQLTVLDVGQGLAVVVRTHEHTLLYDTGPAFTEQIDAGGRIVVPYL
                     RASGIRALDAMIISHDHSDHTGGALSVLQAVPTGWLDSSLPRNHALNLALPQARVCDA
                     GQRWEWDGVSFVFLYPQAADYNSVKDNDRSCVLKISSAYGSVLLPGDIEKYSESRLLQ
                     SSENLRADVLIAPHHGSRTSSTLGFVTAVAPEVTIFSVGYLNRFGHPHPVVNARYRHA
                     GSRTIRTDASGAVLISMNATGISVENWRETHRKYWTGR"
     gene            complement(2936..3640)
                     /gene="lolD"
                     /locus_tag="VN496_17460"
     CDS             complement(2936..3640)
                     /gene="lolD"
                     /locus_tag="VN496_17460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HXF18799.1"
                     /translation="MSEAVVVLCCRGLQKTFRQGQYSVPVLTGVDFEIQRGETVAIVG
                     ASGSGKSTLLHLLGGLDAASAGEIVVMGRRLDQLSEKDKGILRNRGLGFVYQFHHLLP
                     EFTAMENVVMPLLIRRMAAEEATRLASSVLARVGLSARLTHTPGELSGGERQRAALAR
                     ALVTEPACVLADEPTGNLDRHTAEGILDLMLELNRELATSFIVVTHDPMIAARMQRTL
                     RLEDGKLYPASDRMTE"
     gene            complement(3633..>4868)
                     /locus_tag="VN496_17465"
     CDS             complement(3633..>4868)
                     /locus_tag="VN496_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HXF18800.1"
                     /translation="YELFVGLRYTRAKRRNHFISFISVTSMLGIALGVAALIVVLSVM
                     NGFQKELRARILGVASHVQITGMSNGLRDWQRVAKQSLSNPEVIAAAPFVMAQGLISN
                     HQTVQGSIVRGILPSDEDKVAEIGMHMKSGRLGALQAGKFGIVLGSELALSIGARLGD
                     KIVVIAPQGQVTPAGIMPRLKQFDVVGIFEVGMYEYDAGLALINLEDAQKLYRMEDKV
                     SGVRLKLKDLFAAPTIARELTHTLDADVYVADWTRSHANFFRAVQIEKRVMFIILLLI
                     VAVAAFNIVSTLVMAVTDKRADIAILRTLGAAPGEIMKIFIIQGALIGVIGTFVGVVI
                     GMLVAWNVAVIVPAIEHLLGVQFLAKDVYYISDLPSDPHLQDVIVIAFTSFALSLAAT
                     LYPSFRAARINPAEALRYE"
BASE COUNT         1117 a         1450 c         1351 g          950 t
ORIGIN      
        1 tccagttgca acgctcgacc gctggtcttg ctttccgttg cggcttgcct gcgcaaaagc
       61 tgaatctgcg ccatgctcgc gaccccatcc accaaagcaa tcaccgttgc tccgaagaca
      121 cgctgtagtg attcgcgttc cactttcgtt gaaagtggcg ccccaagcag catcattgcc
      181 gcgatagctt cgccgcctac acgcatcgtg ccgagggttc ctgcggcccc gcgagcatgg
      241 tcataccacg cctgtccggt aggatgtaaa agacccggat aatgttgtcg cgcccacgca
      301 tcggcgctga taaccaggtc gcgctccacg ggcgcaagcc gactggtcag cgcttccagc
      361 cactgatctg aagttatgtc gcgaagcagc tcggtggact taaccatctt gtatcgaccc
      421 gtatcatcga ccggtccaat attttctgtg agtctcccgc cagttttcca cgctgatacc
      481 ggtcgcgttc atcgaaatga gcacggcgcc agaggcatca gtacggatgg tacggctacc
      541 cgcgtgccgg tagcgcgcgt taacgaccgg atggggatga ccgaagcggt tgagatagcc
      601 aacggaaaat atagttactt cgggagcaac cgccgtgacg aagccaaggg ttgaagaggt
      661 gcggctgcca tgatggggcg cgataagaac atcggcccgc aaattttcag aagactgcag
      721 caaccgggac tcggagtatt tctctatgtc gccgggtaac aaaacgctgc cataggccga
      781 actgatcttt agcacacaac ttcgatcgtt atccttaacg gaattatagt ctgccgcttg
      841 cggatagagg aacacaaacg atacgccgtc ccactcccat cgctgtccgg catcgcaaac
      901 ccgtgcctga ggcaatgcga gatttagcgc gtgatttctg ggtaacgacg aatctagcca
      961 tcctgtgggc accgcctgca aaaccgacaa ggcgccgcca gtgtggtcgc tgtgatcgtg
     1021 gctgatgatc atggcgtcca gtgcccggat tccggatgcg cgcaggtaag gcactacgat
     1081 acggccgcca gcatcaattt gttcggtgaa agccggtccg gtgtcgtaca gcaatgtatg
     1141 ttcatgtgtg cgtaccacga cagccagccc ctgccctacg tcgagcactg tcagctgcaa
     1201 ttcccggagt ggaacgctgt caggtgccga aaggaaaagt ggcagcatca aggaggcacc
     1261 cagccagcgc gctggaaagc ccttgggcaa taacatccat atcgcacctg cgatggcgat
     1321 cggtatggtc catgccgggg gcgcatgctg ttgccacacg gcggcgggcc agttactgag
     1381 catgttaagt acgatcatgc acagagccat caactgatga gcggcgtaag caagcccgtc
     1441 tacgggaatc actgccgcca gcagtgcaag cggcaccaca atcaggctga ccaccggaat
     1501 cgcgaaggcg tttgcaagcg gagataccag cgatatctgc tggaacagag cgagcaacaa
     1561 cggtatcagg gcgagtgtca ccgccccttg aatctgaatc catgagcgga accagccctg
     1621 tctgccaatt cgggatccgg ccgtcagcat gatgactgcc actgcgccaa acgagagcca
     1681 gaagccgggt gccatgacgg ccatcggatc aagtacaact acgagggccg ccgctgcagc
     1741 gagcacgctg atcggtctag tggtccagtt attccatagc gccaccgcta ccaccgcaag
     1801 catgtaaacg gttcgctgcg cgggaactgc gaaacccgaa agcacggcgt agccgattgc
     1861 gactgcaagc ccgcaaatgg cggcggctcg ccgcgccgga agtttgagca taagtgtttc
     1921 ggacagacgc cacagtcgta gtattagaag tacagccata ctcgccacca tggtgatgtg
     1981 caggccggaa atgctaatga ggtggtttac cccggtgcgc gtgaacacct gccattggtc
     2041 ggccggaatc gcctgctgat ctccaatagc gagtgcgacg acgacgccgg cataagggcg
     2101 ctgacccaat gccgcctgta aatgagcgcg cgccgcttcc cggcatcgtt ccagcaaata
     2161 accggcgcgc cataccagcg gtttgatcaa atgcggcgca ggatccgctc gaacatatcc
     2221 aaccgcgcga ataccgcgct ccagcatcca cgcttcgaaa tcgaatccat ccgggttcgc
     2281 attgccatgc ggccgatgca gacgcacgac gagccgccaa tactggccgg catgcaaggt
     2341 tggcaacgat gcattgtcgg tctggtacca catcagcgaa atgcgcgaag caatcttggc
     2401 attcaatgtg tacgttcgat ccacgtcgag tacaaaacgc accccgcgct cgttaggctg
     2461 caccatctct gcgatgacgc cggaaatctc gatgtcccgc ccttgccaaa tttccggaag
     2521 ttcgtccgaa agtttccaat gagcgatggc cgcagcccag aaaaagccgg ctgccgcaaa
     2581 ggtcacaatt gcggctgcat gcaaggacac tcggttcgcc ggcctcgaag cacgaaacag
     2641 gaacatgcat agcgcggtca atggaaccag tactgcggca gccggccaat gaggcagcac
     2701 gacttgttgc tggagcagcc aggcccctgc aatgaaggcg aacaggtaaa tgctcgtcac
     2761 gatatggcga gattactatt tcacggggat gctgcggaac cttttcgccg aatgccagat
     2821 catcaggcgt tgtgagaatc aggatgatgc tgaaggaagc gccggggtgc ccctcacccg
     2881 cggacggata cggttggttg ccgacattaa agctgcaaac gacaacgctg ttgggttact
     2941 ccgtcatgcg atccgaggcg ggataaagct ttccatcctc aaggcgaagc gtgcgttgca
     3001 ttcgtgccgc aatcatcgga tcgtgcgtca ccacgataaa gctggtcgcc agttcccgat
     3061 tcagttccag catgaggtcg agaatacctt ctgcggtgtg ccggtcgaga tttccggtag
     3121 gctcgtccgc aagcacgcac gccggttcgg tcaccagggc tcttgctaac gccgcacgct
     3181 gacgctcccc cccggacagt tcccctggag tatgggttag cctggcgctg agcccaacgc
     3241 gcgcgagcac gctgctcgca agtcgcgtgg cttcctcggc cgccatgcgc cgaatcaaca
     3301 gcggcatcac cacgttctcc attgcagtga actccggcaa caaatgatga aattgataga
     3361 cgaatcccag cccgcgattg cgaaggatcc ccttgtcctt ctcgctgagt tggtccagac
     3421 ggcgtcccat gactactatc tcgcccgcag acgccgcatc caagcctccc agcaggtgaa
     3481 gcaatgtgct cttgcccgaa ccagatgcgc ctacgatcgc cacggtttcg ccacgctgga
     3541 tttcaaaatc gacgccggtc agtaccggca cgctgtactg cccttgcctg aatgtttttt
     3601 gcaaaccgcg gcaacacaga acaactacgg cctcactcat atctcagcgc ctccgctgga
     3661 ttgattcgcg cggcgcgaaa actcgggtag agcgtggcag ccaggctcaa tgcgaacgag
     3721 gtgaacgcga tgacgatgac gtcctgcaag tgtggatcgg aaggcagatc gctgatgtag
     3781 taaacgtctt ttgccaggaa ctgcacaccg agcaggtgtt cgatcgcggg tactatcacg
     3841 gctacattcc acgcaacgag cattccaatc acgacgccga caaacgtacc gatcacgcca
     3901 atcagcgcgc cctggatgat gaaaatcttc attatttcgc ccggtgcagc acccaatgta
     3961 cgcaggatcg cgatatcggc acgcttatcg gtgaccgcca tgaccagcgt tgaaacgatg
     4021 ttgaatgcgg cgacggcaac tatcagcaat aggataatga acatcacgcg tttctcgatc
     4081 tgaaccgccc ggaaaaaatt cgcgtggctg cgcgtccagt cggcaacgta aacatccgca
     4141 tccagggtat gcgtcaattc gcgggctata gtgggtgcgg caaacagatc cttaagcttc
     4201 aggcgcacgc ctgacacctt gtcttccatt cgatagagtt tctgtgcgtc ctccaaattg
     4261 ataagagcca atccggcgtc gtattcgtac atgccgactt cgaatattcc aactacgtcg
     4321 aattgtttga ggcgcggcat gattccagct ggcgtgactt gcccctgcgg cgcaatgacg
     4381 acgatcttgt cgccaaggcg ggcgccgatg ctgagcgcca gttcactgcc caaaacgata
     4441 ccgaattttc ccgcttgcag tgcaccgagt ctgcccgatt tcatgtgcat gccaatctcg
     4501 gcaaccttgt cttcatccga cggcaaaatg ccgcgcacga tactgccctg cacggtctgg
     4561 tgatttgata tgaggccttg cgccataaca aacggcgccg ctgcgataac ctcagggttg
     4621 ctcagtgatt gtttggccac ccgctgccaa tcccggagac cattggacat gcccgtaatt
     4681 tgcacatggg acgctacgcc gaggatgcgt gcccgcagct ccttctggaa accattcatg
     4741 accgacagca cgacaatcag tgccgcaact cccaaggcga taccgagcat ggaagtaacg
     4801 gaaatgaagg agatgaaatg gttgcgccgc ttggcgcgcg tatagcgcag gccgacaaac
     4861 agctcata
//
